LOCUS       DAEPLF010000001        91331 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEPLF010000001 DAEPLF010000000
VERSION     DAEPLF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 91331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.001"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            35..1444
                     /locus_tag="KR314_000001"
     CDS             35..1444
                     /locus_tag="KR314_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0326415.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIRIFKDKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKICGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            1575..2264
                     /locus_tag="KR314_000002"
     CDS             1575..2264
                     /locus_tag="KR314_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBH0326416.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2267..3202
                     /locus_tag="KR314_000003"
     CDS             2267..3202
                     /locus_tag="KR314_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBH0326417.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3199..4092
                     /locus_tag="KR314_000004"
     CDS             3199..4092
                     /locus_tag="KR314_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBH0326418.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4170..4916
                     /locus_tag="KR314_000005"
     CDS             4170..4916
                     /locus_tag="KR314_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBH0326419.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRASSRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            4917..5750
                     /gene="rsmI"
                     /locus_tag="KR314_000006"
     CDS             4917..5750
                     /gene="rsmI"
                     /locus_tag="KR314_000006"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBH0326420.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            5773..6477
                     /locus_tag="KR314_000007"
     CDS             5773..6477
                     /locus_tag="KR314_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBH0326421.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(6665..7093)
                     /locus_tag="KR314_000008"
     CDS             complement(6665..7093)
                     /locus_tag="KR314_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBH0326422.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7130..8560)
                     /locus_tag="KR314_000009"
     CDS             complement(7130..8560)
                     /locus_tag="KR314_000009"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBH0326423.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            8692..9276
                     /locus_tag="KR314_000010"
     CDS             8692..9276
                     /locus_tag="KR314_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBH0326424.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9292..9813
                     /locus_tag="KR314_000011"
     CDS             9292..9813
                     /locus_tag="KR314_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBH0326425.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    9879..10125
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10209..12146
                     /gene="metG"
                     /locus_tag="KR314_000012"
     CDS             10209..12146
                     /gene="metG"
                     /locus_tag="KR314_000012"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBH0326426.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12176..12946
                     /locus_tag="KR314_000013"
     CDS             12176..12946
                     /locus_tag="KR314_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBH0326427.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGICYEEVCRVTKENAKKFFNIK"
     gene            13283..13714
                     /gene="phnG"
                     /locus_tag="KR314_000014"
     CDS             13283..13714
                     /gene="phnG"
                     /locus_tag="KR314_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBH0326428.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            13729..14304
                     /gene="phnH"
                     /locus_tag="KR314_000015"
     CDS             13729..14304
                     /gene="phnH"
                     /locus_tag="KR314_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBH0326429.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTVSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSTINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14307..15392
                     /locus_tag="KR314_000016"
     CDS             14307..15392
                     /locus_tag="KR314_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBH0326430.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRDFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15412..16281
                     /locus_tag="KR314_000017"
     CDS             15412..16281
                     /locus_tag="KR314_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBH0326431.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16274..17140
                     /locus_tag="KR314_000018"
     CDS             16274..17140
                     /locus_tag="KR314_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0326432.1"
                     /translation="MSKINVSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17181..17894
                     /locus_tag="KR314_000019"
     CDS             17181..17894
                     /locus_tag="KR314_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0326433.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            17895..19064
                     /locus_tag="KR314_000020"
     CDS             17895..19064
                     /locus_tag="KR314_000020"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBH0326434.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDRIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVLNTIVNGKTVYKREV"
     gene            19068..19904
                     /locus_tag="KR314_000021"
     CDS             19068..19904
                     /locus_tag="KR314_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBH0326435.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            19905..20900
                     /locus_tag="KR314_000022"
     CDS             19905..20900
                     /locus_tag="KR314_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0326436.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLDVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            20918..22606
                     /locus_tag="KR314_000023"
     CDS             20918..22606
                     /locus_tag="KR314_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBH0326437.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYIIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            22618..23685
                     /locus_tag="KR314_000024"
     CDS             22618..23685
                     /locus_tag="KR314_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0326438.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24171..24287
                     /locus_tag="KR314_000025"
     CDS             24171..24287
                     /locus_tag="KR314_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HBH0326439.1"
                     /translation="MDDDINMIAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            24451..25518
                     /locus_tag="KR314_000026"
     CDS             24451..25518
                     /locus_tag="KR314_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0326440.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PNSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            25710..26240
                     /gene="rnmV"
                     /locus_tag="KR314_000027"
     CDS             25710..26240
                     /gene="rnmV"
                     /locus_tag="KR314_000027"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBH0326441.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMQRIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            26411..27280
                     /gene="rsmA"
                     /locus_tag="KR314_000028"
     CDS             26411..27280
                     /gene="rsmA"
                     /locus_tag="KR314_000028"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBH0326442.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            27346..28617
                     /locus_tag="KR314_000029"
     CDS             27346..28617
                     /locus_tag="KR314_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326443.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDSDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEEYEELEEDEEIEDNEESDRDEDS
                     EEEYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRV
                     KVQEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGV
                     SQGESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMP
                     YLGYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGST
                     GFLRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            28768..29883
                     /locus_tag="KR314_000030"
     CDS             28768..29883
                     /locus_tag="KR314_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBH0326444.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            30069..30965
                     /locus_tag="KR314_000031"
     CDS             30069..30965
                     /locus_tag="KR314_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH0326445.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASIVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            31035..31832
                     /locus_tag="KR314_000032"
     CDS             31035..31832
                     /locus_tag="KR314_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326446.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(31888..33240)
                     /locus_tag="KR314_000033"
     CDS             complement(31888..33240)
                     /locus_tag="KR314_000033"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBH0326447.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            33459..34337
                     /gene="purR"
                     /locus_tag="KR314_000034"
     CDS             33459..34337
                     /gene="purR"
                     /locus_tag="KR314_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBH0326448.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            34493..34771
                     /gene="spoVG"
                     /locus_tag="KR314_000035"
     CDS             34493..34771
                     /gene="spoVG"
                     /locus_tag="KR314_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBH0326449.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            34981..36360
                     /gene="glmU"
                     /locus_tag="KR314_000036"
     CDS             34981..36360
                     /gene="glmU"
                     /locus_tag="KR314_000036"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBH0326450.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEAEVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSNVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SVVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            36455..37405
                     /locus_tag="KR314_000037"
     CDS             36455..37405
                     /locus_tag="KR314_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBH0326451.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      38245..38330
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            38492..39013
                     /locus_tag="KR314_000038"
     CDS             38492..39013
                     /locus_tag="KR314_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0326452.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNDEGVTLTEAQSAKLLSDIGENKIYTSVTADNLGNPLTSNTKVDNKVLY
                     IVLIDNTVMDSTK"
     gene            39164..40840
                     /locus_tag="KR314_000039"
     CDS             39164..40840
                     /locus_tag="KR314_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBH0326453.1"
                     /translation="MAKKVRIGDKLVEKGYITEDQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            40852..42060
                     /locus_tag="KR314_000040"
     CDS             40852..42060
                     /locus_tag="KR314_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBH0326454.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            42075..43787
                     /locus_tag="KR314_000041"
     CDS             42075..43787
                     /locus_tag="KR314_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBH0326455.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETHDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            43780..44688
                     /gene="pilO"
                     /locus_tag="KR314_000042"
     CDS             43780..44688
                     /gene="pilO"
                     /locus_tag="KR314_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBH0326456.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            44715..45281
                     /locus_tag="KR314_000043"
     CDS             44715..45281
                     /locus_tag="KR314_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0326457.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            45318..45845
                     /locus_tag="KR314_000044"
     CDS             45318..45845
                     /locus_tag="KR314_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0326458.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            45866..47404
                     /locus_tag="KR314_000045"
     CDS             45866..47404
                     /locus_tag="KR314_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326459.1"
                     /translation="MRKWNKFKSERGAALVLVLIVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEKNIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            47504..48586
                     /locus_tag="KR314_000046"
     CDS             47504..48586
                     /locus_tag="KR314_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBH0326460.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESIFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            48567..49349
                     /locus_tag="KR314_000047"
     CDS             48567..49349
                     /locus_tag="KR314_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBH0326461.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGILFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILIFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            49451..50101
                     /locus_tag="KR314_000048"
     CDS             49451..50101
                     /locus_tag="KR314_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBH0326462.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            50115..50675
                     /locus_tag="KR314_000049"
     CDS             50115..50675
                     /locus_tag="KR314_000049"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBH0326463.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            50692..54078
                     /gene="mfd"
                     /locus_tag="KR314_000050"
     CDS             50692..54078
                     /gene="mfd"
                     /locus_tag="KR314_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBH0326464.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGAENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            54109..55104
                     /locus_tag="KR314_000051"
     CDS             54109..55104
                     /locus_tag="KR314_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0326465.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            55228..55767
                     /gene="spoVT"
                     /locus_tag="KR314_000052"
     CDS             55228..55767
                     /gene="spoVT"
                     /locus_tag="KR314_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBH0326466.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            55887..57500
                     /locus_tag="KR314_000053"
     CDS             55887..57500
                     /locus_tag="KR314_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBH0326467.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            57607..59055
                     /gene="mazG"
                     /locus_tag="KR314_000054"
     CDS             57607..59055
                     /gene="mazG"
                     /locus_tag="KR314_000054"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBH0326468.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            59144..59422
                     /locus_tag="KR314_000055"
     CDS             59144..59422
                     /locus_tag="KR314_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBH0326469.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            59494..59736
                     /locus_tag="KR314_000056"
     CDS             59494..59736
                     /locus_tag="KR314_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBH0326470.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            59798..60052
                     /locus_tag="KR314_000057"
     CDS             59798..60052
                     /locus_tag="KR314_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBH0326471.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            60053..60586
                     /locus_tag="KR314_000058"
     CDS             60053..60586
                     /locus_tag="KR314_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326472.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            60592..60876
                     /locus_tag="KR314_000059"
     CDS             60592..60876
                     /locus_tag="KR314_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBH0326473.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            60989..61909
                     /locus_tag="KR314_000060"
     CDS             60989..61909
                     /locus_tag="KR314_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBH0326474.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            61999..64371
                     /gene="spoIIE"
                     /locus_tag="KR314_000061"
     CDS             61999..64371
                     /gene="spoIIE"
                     /locus_tag="KR314_000061"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBH0326475.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNNEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            64722..66614
                     /gene="pepF"
                     /locus_tag="KR314_000062"
     CDS             64722..66614
                     /gene="pepF"
                     /locus_tag="KR314_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBH0326476.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAIAVMNNN
                     DTEDDAEKKQEVDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMSDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESVDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            66728..67693
                     /gene="galU"
                     /locus_tag="KR314_000063"
     CDS             66728..67693
                     /gene="galU"
                     /locus_tag="KR314_000063"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBH0326477.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            68193..69797
                     /gene="rho"
                     /locus_tag="KR314_000064"
     CDS             68193..69797
                     /gene="rho"
                     /locus_tag="KR314_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBH0326478.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQENRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            69950..70150
                     /gene="rpmE"
                     /locus_tag="KR314_000065"
     CDS             69950..70150
                     /gene="rpmE"
                     /locus_tag="KR314_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBH0326479.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            70295..71188
                     /locus_tag="KR314_000066"
     CDS             70295..71188
                     /locus_tag="KR314_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBH0326480.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            71206..72054
                     /gene="prmC"
                     /locus_tag="KR314_000067"
     CDS             71206..72054
                     /gene="prmC"
                     /locus_tag="KR314_000067"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBH0326481.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            72098..73162
                     /gene="prfA"
                     /locus_tag="KR314_000068"
     CDS             72098..73162
                     /gene="prfA"
                     /locus_tag="KR314_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBH0326482.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            73291..73971
                     /locus_tag="KR314_000069"
     CDS             73291..73971
                     /locus_tag="KR314_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBH0326483.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            74000..75040
                     /locus_tag="KR314_000070"
     CDS             74000..75040
                     /locus_tag="KR314_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBH0326484.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            75057..75506
                     /locus_tag="KR314_000071"
     CDS             75057..75506
                     /locus_tag="KR314_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBH0326485.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            75523..75975
                     /gene="rpiB"
                     /locus_tag="KR314_000072"
     CDS             75523..75975
                     /gene="rpiB"
                     /locus_tag="KR314_000072"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBH0326486.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            76011..76640
                     /gene="upp"
                     /locus_tag="KR314_000073"
     CDS             76011..76640
                     /gene="upp"
                     /locus_tag="KR314_000073"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBH0326487.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            76940..77755
                     /locus_tag="KR314_000074"
     CDS             76940..77755
                     /locus_tag="KR314_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBH0326488.1"
                     /translation="MKNCIAVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            77981..78418
                     /locus_tag="KR314_000075"
     CDS             77981..78418
                     /locus_tag="KR314_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBH0326489.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            78603..78830
                     /locus_tag="KR314_000076"
     CDS             78603..78830
                     /locus_tag="KR314_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBH0326490.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            78835..79221
                     /locus_tag="KR314_000077"
     CDS             78835..79221
                     /locus_tag="KR314_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBH0326491.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAIRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            79224..79928
                     /gene="atpB"
                     /locus_tag="KR314_000078"
     CDS             79224..79928
                     /gene="atpB"
                     /locus_tag="KR314_000078"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBH0326492.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            79997..80257
                     /gene="atpE"
                     /locus_tag="KR314_000079"
     CDS             79997..80257
                     /gene="atpE"
                     /locus_tag="KR314_000079"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBH0326493.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            80380..80877
                     /gene="atpF"
                     /locus_tag="KR314_000080"
     CDS             80380..80877
                     /gene="atpF"
                     /locus_tag="KR314_000080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBH0326494.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            80874..81419
                     /locus_tag="KR314_000081"
     CDS             80874..81419
                     /locus_tag="KR314_000081"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBH0326495.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            81436..82938
                     /locus_tag="KR314_000082"
     CDS             81436..82938
                     /locus_tag="KR314_000082"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBH0326496.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            82966..83844
                     /gene="atpG"
                     /locus_tag="KR314_000083"
     CDS             82966..83844
                     /gene="atpG"
                     /locus_tag="KR314_000083"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBH0326497.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            83857..85251
                     /gene="atpD"
                     /locus_tag="KR314_000084"
     CDS             83857..85251
                     /gene="atpD"
                     /locus_tag="KR314_000084"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBH0326498.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            85254..85514
                     /gene="atpC"
                     /locus_tag="KR314_000085"
     CDS             85254..85514
                     /gene="atpC"
                     /locus_tag="KR314_000085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBH0326499.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            85976..86356
                     /locus_tag="KR314_000086"
     CDS             85976..86356
                     /locus_tag="KR314_000086"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBH0326500.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            86371..86832
                     /locus_tag="KR314_000087"
     CDS             86371..86832
                     /locus_tag="KR314_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBH0326501.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            86853..87443
                     /locus_tag="KR314_000088"
     CDS             86853..87443
                     /locus_tag="KR314_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326502.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            87458..88615
                     /gene="alr"
                     /locus_tag="KR314_000089"
     CDS             87458..88615
                     /gene="alr"
                     /locus_tag="KR314_000089"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBH0326503.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            88648..88935
                     /locus_tag="KR314_000090"
     CDS             88648..88935
                     /locus_tag="KR314_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBH0326504.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            88904..89287
                     /locus_tag="KR314_000091"
     CDS             88904..89287
                     /locus_tag="KR314_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBH0326505.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            89458..90285
                     /locus_tag="KR314_000092"
     CDS             89458..90285
                     /locus_tag="KR314_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBH0326506.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            90278..91219
                     /locus_tag="KR314_000093"
     CDS             90278..91219
                     /locus_tag="KR314_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBH0326507.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGINAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
BASE COUNT        36809 a         9837 c        16851 g        27834 t
ORIGIN      
        1 gaagtattat gtaaaaaagg agtactatta atatatgttt attattaatg aattaaataa
       61 gattgtaaat gaagaagtaa tatcttttca tatgcctggt cataaaaagg gacaggttta
      121 taaaaagtta ggatataaag atatactaaa aaatttgtat aaaatggata caactgagat
      181 attaggaaca gataatttac actttccgga aggaattata aaaaattcac aaaaaaaagc
      241 atcaagagta tttaaaagtg attatacttg ctatttagta aatggtagta gctgtggaat
      301 acaatctgca ataatgtcag tatgtagtcc taaaagtaaa ataataacaa atagagactg
      361 tcatcagtct gttataaatg gatgtatttt aggagatata gatatagagt atgtgccttg
      421 tgagataagt gaagagacaa atgtcttaaa agggatatct ataagtaatg tgattgatat
      481 aatagataaa aacttggatg caaaagcagt actgcttact tatcctacat attatggaat
      541 gacatatgat ttagaataca tatgtaatta tgcacatagc aaaaagatgg tagtgatagt
      601 agatgaagca catggtgcgc atttagggct tagtgagagg cttcctaaaa cagctcttga
      661 gcaaggtgca gatatagttg tacagagtac acataaaact cttccagcat ttacacaatc
      721 gtcaatgata catatacagg gagaccgagt tgatatagat aaaatattga acatgttaag
      781 aattacagaa tcttcaagcc cttcatattt attattatct tctctggaat tagcagtaga
      841 tatatatgaa aataaaggaa aagaattaat ggaagagctt ttaaataata ttagaatttt
      901 caaagataaa gttgattcaa atattaatat atatgacaca aatgataaaa caaaaatatt
      961 tatatcatct aaaaacatgg gtttaacagg ttatgaacta gaaaatattt tgagaaacaa
     1021 atataacata caagtggagt tatctaatta ttatggagtt cttttaatat gtacaatagg
     1081 aaatactgct gaggatttct ctagtttaga aacagcatta aacaatgttg taaaggaaga
     1141 ctttaaattt gaaaagttag aaaataaaaa ataccctgtt gttattccaa agaaaatatt
     1201 aacacctaga gaagcatttt ataaaactaa aaaaagtgtt aaaatatatg acagcatagg
     1261 taaaatttgt ggtgaatgta tagttccata cccacctgga ataagtgtaa tatctcctgg
     1321 tgaagtaata agtaaagaaa taatagacta tctaatgttt tgtcattcta aaggcatgat
     1381 tgtgagtgga ttgaaagatg tagatttaaa ttttatacaa gtaatagatt tagaagacat
     1441 ttaaaaatta tgatataaaa gtagatagaa gtagatagtt taaaattgtg aagagaaata
     1501 aatagactgc ttatatagag ctatctaata aaagtactaa aaaagtaaaa agtgtttgta
     1561 tggaggatat gaaaatgaaa ggtaagttaa taattataga gagtggttca gatgcaagtg
     1621 gaaaggctac tcagacagaa atattatatg aaagattaaa aaaagaaggc tatatgatta
     1681 aaaaggtaga atatccaaat tatgaatctc catcatctgc cttagtgaaa atgtacctaa
     1741 aaggagactt tggtaaaaat gcatcagatg tagacccata tatagcatca acttttttta
     1801 cagctgatag gtatgcatca tttaaaaccg attgggagga gttttacaat gaaggtggaa
     1861 taattatcgc agatagatat acaacctcta atatggtaca tcaggcctct aaaatggata
     1921 tttgtgatag agaagagtat ttaaattggt tagctgatta tgaatttaat ttatttaaga
     1981 taccacaacc tgattgtgtt atatttttag atgtgcctat tgaatttagt aaaaagctta
     2041 tggagaatag aaaaaataaa attacagggg aagaaaaaaa ggatattcat gaaagtgaca
     2101 tagagtattt agagaaatct tacaacaatg ctttatatat agcagataaa tatgattgga
     2161 aaaaaataaa ttgtgtttta gataacaaat taagaagtat tgaaagtata cataatgaaa
     2221 tttataaaat agttcttgat tcaataaaac ctatggagag ataaatatgt attttgaaaa
     2281 tattataggg caagattttg caaagaaata tttaacaaat tctataaaga aaaataaact
     2341 taataatgcg tatatgtttg aaggaatgga tggaatagga aaaaagaaat ttgcagatga
     2401 attatcaaaa ctattacttg attatgaaaa tctcgaaaat agtccagact atgtactcat
     2461 aaaaccagat ggaaatagta taaaaatagc tcaaatcaga aatttacaat cagatatagt
     2521 aataagacct cataaagact ataaaatata tataatcaat aatgctgaaa aaatgactgt
     2581 ggaagctcaa aatgcacttt taaagacgct agaggaacct ccaaactatg ctataataat
     2641 cttagtcaca aacaacaagg aatctttatt agaaactata aaatctagat gtgatataat
     2701 aaagttctca cctataccaa ttgaagactt aaaacgttat ttgataaata caggtataga
     2761 agaagaaaga gctcaattat tggctatttt ttcgagagga agtattgaga atgctctaaa
     2821 tctatctcag tcttctgagt tttcaatgat gagagaagat atacaacagt atattcagat
     2881 aatgttagat aaaaacatag tggagatttt aaatatacca aacaatatgg aaaaatatag
     2941 aggtaagatt atagctttat tagatatgat gataaactat tttcgagata tcatcctatt
     3001 aaaagaaaat gtaaacaaga acatgttaat aaatgtagat aagctagttt tcattcaaaa
     3061 tatgagtgga aaaattagtt attctcaatt gtccaagatt attgatataa tagaagatgc
     3121 aaagagtaaa ataaaaagca attgcaattt caatataagc atacaagtta tgtctttaaa
     3181 tatatacgag gtgattaaat gataaagata gtaggtataa ggtttaaaag tgctgggaaa
     3241 atatattatt ttgaccctgt tgattttaat atagagcaag atatggatgt agttgtagaa
     3301 actgcaagag gcttggagta tgggaaagta gtcgttggtc caaaagatat ggatgaaagc
     3361 gaattagttt ctcctttgaa gccaattata agaatagcta cagaagaaga taaaaaagta
     3421 tatctagaaa ataaggaaaa ggcaaaagaa acttttgagt tgtgccaaca aaaaataaaa
     3481 gaacatgagt taacaatgtt tttaatagat tgtgagtata cgtttgatag gaataagttg
     3541 attttttact ttacagcaga aggaagaata gattttagag aattggtaaa agacttagct
     3601 gctatattta aaactagaat agaacttaga caaataggtg tcagagatga agctaaatct
     3661 ataggaggtc tagggccttg tggtagaaaa ctttgttgtt catcttggtt aggtgatttt
     3721 caaccagttt caataaaaat ggcaaaagac caaagtctat ctctaaatcc aacaaaaata
     3781 tctggaatat gtgggagatt attctgttgc ttaaaatatg agcatgatgt atatagtgaa
     3841 gcaatagaaa aaatgcctgt tgtaggtgca ttggtgcaat cagatgatgg aaaaggaaaa
     3901 gttgtagaag tgaatccttt attagaacaa attaaagtgg aatttcaaga caagacagta
     3961 aagatataca caagagaaga tataaaaata cttagagagc ctaaaaaatg tgatggctgt
     4021 ggtggtaaat gtaaggataa tgatggatta gatgaagcca cactaagaga gctaaagaaa
     4081 ttagaagatt aaatttaagg tagggctttt cctacctttt ttaaagcaat taatgcttaa
     4141 agttattaat atttgaggag aatttatata tgcaattaaa agattcagaa agaatagatg
     4201 atttacaatt aaaaggcctt aaaattatac aagatactaa tggtttttgt tttggtatag
     4261 atgcagtttt attagccaat tttactaaaa taaaaaaaga tgccaaagta gtagatttgg
     4321 gtactggaac tggaattata cctattttga tagctggaaa aagtgaagct aaaaaaatta
     4381 ttggcgtgga aatacaagaa gatgtatatg aaatggcaac tcgttctata aaattaaatg
     4441 gattagaaga aagagttgag attataaatg aagatataaa atacatagac aaagtcttag
     4501 acataaatgg ttaccatgta gtcacatcaa atcctccata tatgcatatt gatggaataa
     4561 aaaatccaaa tgataaaaaa gcaatatcaa gacatgaagt aaggtgcaat ttggaagatg
     4621 taattagagc ttcatccaga ttggttatgc ctagaggaaa attcttcatg atacatagac
     4681 caacaagatt ggttgatata ataactttag ggagaaaata taaattagaa ccaaaggtta
     4741 ttcaatttgt gcatccaaga cctcaaaaag caccgaactt agttttggtt caatttgtaa
     4801 aagatggaag accagagctt aaaatattgg acccattgta tgtttatgga gaagatggga
     4861 attatacaaa agaattaaaa gcaatatata ataataaaga tataggagag ttgtaaatga
     4921 gtgggaaatt gtacatatgt ccaacaccaa taggaaacct agaagatata acatatagaa
     4981 ctttaagaac attaaaagaa gtagacttga ttgcagcaga agatacaaga catagtataa
     5041 aacttttaaa tcattttgaa atctcaaaac cactaactag ttatcatgaa cataataagg
     5101 attcaaaggg agattatttg ataaataaac taattgatgg tgaaaatata gctctgataa
     5161 gtgatgcagg tatgcctggt atatctgacc caggagaaga gataataaaa caagctatac
     5221 aaaataacat agagatagaa gtattgccag gtgcaacagc ctttgtaacg gctttagttg
     5281 gctctggtat ggacacacat agatttgttt ttgagggctt tttagataga gataaaaaag
     5341 ttaggaaagc acaattggaa gaattaaagg aagaaagtag aactataatt ttctatgaat
     5401 caccacatag attgaaggat acactaaaag atatgcttaa aattcttggt aataggaata
     5461 tttctataaa tagagaactt acaaaaaaat atcaagaaat aataagagaa aatatagaaa
     5521 ctaccataaa aatatttgag gaaaaagaag taagaggaga gtttgtatta atagtagagg
     5581 gttttcatgg tgagaagtca aagaaagaaa attatgatgg gcttacagat agggaatatg
     5641 ttttaaaact aattgaaagt ggtatgaaca aaaaagatgc aataaaaata gtttgtaaag
     5701 atagaaagtt aaagaaagat gtggtttata aacaagtttt agatttataa aacctgaatc
     5761 tgaggtgatt ttatggaaat agggaaaatt ataatagaga gtttaaagag ggaagataca
     5821 cataaaatat ttatagaatt agataataag catgaattag aaaaaataat tcctaaaata
     5881 agctgtatga agtctgtagg tgaatgtaaa tatcatgtag taaattgttt tgaacattct
     5941 ataaatgcat taaaagaatt agaaatagta ttaaatgata aggatttttt tccaatacat
     6001 ctaagaaaac atgtgtcaaa ctatttaaac acatatattg aagaaggaat aaataagtta
     6061 catgtattaa agttgggaac atttttacat gatataggta agcctgatag tatgacctta
     6121 gatgaaaccg gaagagttca tttcacaaat catgaaaaaa taggtgctca aataattgac
     6181 aatatgggaa taaagttaga gttatcaaca gagacatata gacttataag taaatatgta
     6241 agatatcata tgatactttt gtctctatat aagaaaaatg atttgagtag gaaagaatta
     6301 attaatgtat ttaacttagt agatgaggac actataggtg taataatgct tgggtatgca
     6361 gatatagtgt ctacagtaaa attattgggc aagagtggag aagtcagtgt gttaaagaca
     6421 tatatggaat atattttaac aaattacttg tataagagta attatacaca tgtataaatt
     6481 tagttaatat aattgagttt tagatgatta attgaagttt gaaatatacg gatttcaaaa
     6541 acataaaaga agttgtcttt gttgtaaaat tatgaaagac aacttttttt atatcaaaaa
     6601 ttgaaatttt attaaggata cttaatggtc tattaattaa taaaccatta gtatttaatt
     6661 tatattacaa tattgatttt gcttgtattg gcgttgatag tataacagag gtcttagtag
     6721 aaccaatttt ctttatagta tcaatcacat tttctagctc gtacatatct tttacaatta
     6781 cttttaaaag taagcaatca tcacctgtta tatggtgaca ttctacaatc ctagggtcct
     6841 ttgcagctga ctcaataaat tctgtatatc cattgcttgg aagagaaata tgaataaatg
     6901 cctttataac tctgcctaat gaatctgggt tgacaatagc tttatatcct tctataacac
     6961 cagactcttc taatcttttg actctttctg aaactgcagg agaagttaaa ccaactattt
     7021 ttcctaagtc cttcatagaa attctcccat catcctgtaa aatttctatg attctgtaat
     7081 ctgtaacatc cataaataaa cagtccccct taaataaaaa taattaatac tataaaactt
     7141 ttttagaatc tatcatttgt gtttttaaat accaaagatt tctagataaa tcaaccttat
     7201 aaatgtgtgg ttttgaaagt cttttgtatt gttcccagat agtacttaat tcatattgta
     7261 catgattttt tatttcaaga acagcttttt tattatattt gcatctgcct tttatataca
     7321 atggtttaag aagctcttta actttgtaat ttgtaaatac ctttgttttc catgtataag
     7381 taggatggaa gatttctaaa ggtttagact catctataac ttcatcatgt aacataatca
     7441 aatctgcctc tgccttattg tcttcgttat atattcttat aactttcttg taaccaggat
     7501 tatttatttt ttctggattc tcagaaatct ttatcttagg ttcaagaact cctttttcac
     7561 aagatgcagc taatttataa actcctccaa gagaaggtga atcgaaagat gtaatgagct
     7621 ttgtacctac tccccatgaa tttatagtag ctccttctgc tttaagtgat gttatggtat
     7681 attcgtctag gtcattagat gcagtgatgc tgatatctga aaagccagcg ttatctaatt
     7741 gttttttagc ttctttagat aaataagcta aatctccaga gtcaagtctt atacccatag
     7801 gtttgtatcc tttttcagaa atatttttga atacttttat tgcattagga actccactgt
     7861 ttaatacatc ataagtatct acaagtagta ggcatttatc tggataaaca tcagcatatg
     7921 cttggaatgc ttcaagttca ttatcaaatt tttgtaccca actgtgagct tgtgtaccaa
     7981 ttataggtat atcaaacatt ttccctgcta gtacgtttgc agttccagca caaccaccaa
     8041 ctacagcagc cctagctcca tatatccctg catctggacc ttgtgcacga cgtaatccaa
     8101 attcaaatac agggtctcct tgagctgcaa agcaaactct agaggcttta gtagcaataa
     8161 gagactgaaa attaactatt gttaacaatg ctgtttcaat aagttgagct tgatataaag
     8221 gtgccttaac agtaattaat ggctcattag ggaacatgat agttccttcc tcaacagcat
     8281 atatatctcc tgtgaattta aattctttta aaaattttaa aaacttgtca gaaaataaat
     8341 ttaagttttt taagtactct aaatcttcat ctgaaaaatg aagattatct atgtattcaa
     8401 caacctgatc aataccacaa actatagtat accctccatc acaagcgttt tttctaaaaa
     8461 acatatcaaa tacaacaatg tcttcatgaa tattcttttc aaaatagcca tttaacatag
     8521 taagctgata taaatcagta agaagtgtta gatttctcat tatttctctc cttaattatt
     8581 attaaatatt aaaaattgta aaaactccca cacttatact aaaacataat atagtacaac
     8641 tttcaagtat aaagtattac ctaactatca taaaattatt taaagaggag gatgattata
     8701 ataggaaaaa tatggtttta tatggtatcg ataggaataa taggaagctt gatttctaaa
     8761 aatatgggtg aattaaataa agttatatta agtgaaacat caaaaggtat agagtttgca
     8821 ataagtctag ctggtatcat ggcactctgg atggggataa tgaatatcgc aaaagaatca
     8881 ggtttaattg aaaagatagg tcaaaaacta aatcctataa tgagaagatt atttccttct
     8941 attccaaagg gtcataaagc aatgtcttat atagttatga atattgcttt aaatatgttg
     9001 ggtgctggaa atggtgcaac agcatttggt ttaaaagcaa tgagtgagct tcaaactttg
     9061 aataacaaga aagatacagc cactaatgat atgattatgt ttatggtaat caatatatca
     9121 tctatacaaa taattccatt tactataatc aaattaagga tggatatggg ggctcaaaat
     9181 ccaagtgaaa taatttttac gactttattt gcaaccataa tatctacagc agtagcaata
     9241 ataacatgta aattttttca aagaagatat agataaaggg gatgtagata catggagata
     9301 ttttcaaact tacttatacc aataataatt ctgtacatag tagtttatgg taaatataaa
     9361 aaaatagatg tgtatgatag ttttgtaaaa ggagcaattg atggattaaa agcagcatgg
     9421 gatatcctac catatataat aggtatattt ttggcaatag gaatattcaa aactggtaaa
     9481 ggtcttgata tgttagaatg gctatttaca ccaatagcaa atatgatgag tataccaaaa
     9541 gaattaatag gtctaataag tgtaaaacct ttatctggaa gtggtgcttt agggatgtac
     9601 agtgaacttg caaatagagt tggaatagga agtttagtag aaaaaatggg tgctacgatt
     9661 gtaggttctt ctgagactat tttttacaca atggcaatat actatggaag tctgaagata
     9721 aaaaatacta gacatacatt atcatgtgct atgattagcc atgttgcagg tgtcatagca
     9781 gctgttttta tttgctatgt aatatttgta taatttgttg acaatatggg aaaaatttta
     9841 taatatagtg attatataga aacaaatagt aatacaaatt acaagctatg aaaagaagag
     9901 taaataaagg ttttttctat cagagagcta ggttaggtga aagctagtgt caaaagcttt
     9961 attgaagatg gtcttggagc tttttatctg agcaagtgtg ctagggtaaa acggttggac
    10021 acgttatagt ctactaaggt atctataaaa tgtttctaca tttcatgtga aacataacta
    10081 ttatagatga attagggtgg taccgtgaat aatctcgccc ctatgttaac tcataggagg
    10141 cgatttttta ttatttaaat gaaagaatca tatgattacg aacgataaaa aattaggagg
    10201 taaataacat gagcaaaccg agtttttatg taacaacacc aatatactat ccaagtggag
    10261 gattacacat aggacatact tattcaacag tagctgcaga tacaatagct agattcaagc
    10321 gtttctgtgg atatgatgta aagttcttaa caggaacaga tgagcatggt gaaaaaattc
    10381 aaaagaaagc catagaacaa ggaatgtcag agatagagta tcttgatgga atgataaaag
    10441 atataaaggc cttatggaat actatggata tatcttatga tgattttata agaactacag
    10501 aaaaaagaca tacagacata atacaaaaga tatttactaa actatatgag caaggtgata
    10561 tatataaagg tgaatatgaa ggtagatact gtactccttg tgagagtttc tggacagaat
    10621 ctcaattact agaaggaaat aaatgccctg actgtggaag agaaacatat ttagtaaaag
    10681 aagaatctta tttctttaga ttatctaaat atgaagacag attaaaagag ttatttaaag
    10741 atgatagttt ctgttttcct gctgctagaa aaaatgaaat ggtggcaaac tttttagaca
    10801 aaggattaga agatttaagc gtaactagaa cgacttttga ctggggtata aaagttcctt
    10861 ttgatgaaaa acatgtaatt tatgtgtggg tagatgcttt atgtaactat ataacagcat
    10921 taggttatat gactgataat gatgaagaat ttaaaaaata ttggcctgcc aatgttcaaa
    10981 tagtaggtaa agaaatagtg agattccata caataatatg gccagcgctt ttaatggctt
    11041 taggtctaga agttccaaaa caagtatttg gacatggatg gatactattt gctgatgata
    11101 aaatgagtaa atcaaaaggt aatgtggttt atccagaacc tataatagaa agatatggaa
    11161 tagacacact taagtacttc ttattaagag agtttgcatt tgggcaagat ggaagttata
    11221 ctcatagaaa ttttgtaacg agaataaatt atgatttagc aaatgattta ggaaacttaa
    11281 taagtagaac tgtagctatg gttgaaaaat acaataatgg tataatacca acagcaaaag
    11341 tttcaactga ttttgatgct gacttaaaag aacaagctgt ttcaactaga gaaaactttg
    11401 aagctgaaat ggataaaatg caattccatg aagcattaga gagtgtatgg aaattagtta
    11461 gaagaactaa caaatatata gatgaaacta tgccttgggc actagctaaa gatgaaacga
    11521 agaaagatga attagacaca gttttatata atttatgtga atctataaga ataatcgcaa
    11581 cactaataaa tcctataatg aatgagactg ctaataaaat atatgaacat ataggaataa
    11641 aaggtcaaga tgatataact acatgggaaa gtacaaaaac atttggtctt attggagaaa
    11701 atgtaaaagt atttaaaggt gaacctttat tcccaagatt agatgtagaa aaagagatag
    11761 aagaattaac taaaatgttc tctgggaaac ctccagtaga agaaaaacct ttagagcata
    11821 aagaagaaat tactattgat gatttagaca aaatagagct tagagtaggg aaaataataa
    11881 gctgtgaaaa acatccaaaa gcaaataaat tattagtatc acaagttaag attggaccag
    11941 aaacaagaca aatagtatca ggaatagctg aatactataa gccagaggat ttagtaggaa
    12001 aagaggttac agtggtatgt aacttaaaac cagttaaatt aagaggtgta gaatctcaag
    12061 gtatgatatt agcagcaggt gatgatggag acccatatgt tttaccattt acacaagggg
    12121 ctaaagatgg atgcgaggtt cgttagtagg taattaaata gtaaggagat taaagatgtt
    12181 atttgactca catgcacatt taaatgatga aagttttgat gaagatagag atgaacttat
    12241 tggttcatta aaagataaag gtgtagattt ggttgtaaat ccaggagcag atatagagac
    12301 gtcgataaca gcaattgaac ttgcgaaaaa atacgacttt atatattcag cagttggggt
    12361 acatccacat gatgtatcaa aactagatga tacagcaata gaaactctaa gaaaacttgc
    12421 aactgagaat gaaaaagttg tagctattgg agagattgga ttagattatt attatgatta
    12481 ttctccaaga gaagaacaaa aagagtggtt taagaagcaa attgaattag ctaatgagct
    12541 aaaacttcca ataataatac atgatagaga tgcacatgga gatacttttg agataataaa
    12601 gaatactaaa aatcctgaaa taggatgtgt acttcattgt tatagtggaa atgtagagtt
    12661 agctagagaa tatgttaaga tgggttgtta tatatcaatt ccaggcacgg ttacttttaa
    12721 gaataataaa aaaactagag aagtagtaag agaaatacct ctagaaagat tattcataga
    12781 gactgattca ccatatatgt caccagagcc acatagagga aagagaaaca atccttctca
    12841 agtatctttt gttgctgata agatagccca ggaaaaaggg atatgttatg aagaagtatg
    12901 tagagttaca aaagaaaatg caaagaaatt ttttaatata aaatagacaa attaaatctt
    12961 ataatgatat atataataag tcattataag tataggatga gagtgcctca aaatgagttt
    13021 tttagttcat gaggcactct ttttttatga aatcaaatat attttcatca tttcaacaat
    13081 gaaaaagaag tttatcatat tttgagacag tttgttttta tttgtttaat tcatatttta
    13141 aaatatattt tcttaacatt gttaaaaaag aatttacaag atattcatta gaattaactc
    13201 aactctaata ttcacttaac ttaagtggtt tattatatta ttaaagagaa gtaaacgaaa
    13261 ggtagattaa ggaggctaaa ttttgtatac agttgatagt actaagatat tgataaatat
    13321 gaacttagag aaactagaaa agatatactc aatgataaaa gatacatcaa gtatttctat
    13381 aattaaagaa cctaacttgg ctacagtaat ggtaagagct aatgaaagtg taaaaaatac
    13441 aacatttaat ttaggagaga tattagttac tgaatgtagt gtaaaggtag atgaaagctt
    13501 gggttacgga atagtttcag aaaacaataa taagaaagcc atttacttag cagttataga
    13561 tgcagtttta catagcaata acagtaaatt tgatgagtta aagaattaca taaataaaag
    13621 tgtatatgaa gagaacttaa ggtatgaaca agagattatt gatgaattta gcttgattaa
    13681 taaaacgaaa gttcaattca atgctatgga ttaaatagga ggagaatcat ggtaaattat
    13741 acacaagaaa tatatagaaa gatgctagat agtgcttcgt ttcctgggaa attaaataat
    13801 atagataata taaatataga aaataataca aaactattga atggagcaat tggtatagca
    13861 atcactttat tagaccaaga agttaccttc tatatagaaa attatactca aaaagatata
    13921 aaaaatataa gagcgcttac agtgtcaaat caaatagatt atagaaaatc agattatata
    13981 tttttagata ttaattcaga agtggatata ttacagatta aaataggttc ttttgaatat
    14041 cctgatgaaa gtgcaactat agtacatcaa gtaaatgatt tatcaacgta ctctcaaact
    14101 aagtatataa agcttgattt gagtggtcct ggtataaaat ctaaaaatag tatatatata
    14161 aatggaatta gagaagaatt tatagaaaga ttatctacca taaataaaga ttatccaata
    14221 ggaatagatt tgattttagt agataaaaaa ggagaaattg catttatacc aaggtcatca
    14281 aaattaagtt gggaggtatt gtagaaatgg cttatgtagc agttaaaggt ggagaagaag
    14341 ccatattaca ggctaaaaat atacttgagt tttatagggt aaaaggagat agcttgccta
    14401 tagatgtaaa gcagattgag gaacaaatgt cttatgctgt agacaaagta atgtctgaag
    14461 gaagtttata caacaaggaa ctatcagcat tggctataaa acaagctcaa ggtgatatat
    14521 tagaagcctc tttctatgta agagctttaa ggtcaacaat gcctagaatt acatattcag
    14581 aacccataaa tacagaagaa atgagaataa aaagaagaat atcagctact tttaaagata
    14641 taccaggagg tcagtatctt ggggcaacta gagactatga aaatagaata ttaaatgtag
    14701 aattattaga agaagatgaa aaaaagaata gagattttag agatagtttt ataaatgaat
    14761 tagaaacagt agatgaaatc cctgaattta caaaaatatc taatatattt agaaaagaaa
    14821 atttattaca agaagaagtt tgtgaaaaca aattggaaga agttattgat gatattacaa
    14881 gaaatagttt aagatttcca gtttctaggt cagcaaagtt acaggcattg actagaggtg
    14941 aaagtggagt agtgtcaggt tttgcttatg ctattgtaag aggttttggt gatgaacatc
    15001 catatataag tgaacttaga actggtaatg tatttataaa agtaaaacat cccatctatg
    15061 aagatgaaga aataataata ggtgatatgc tggttactga atgtgaaata gtttcaaaag
    15121 catcacaaaa taagactgaa aattctaaaa ctaattcgga agataaaaat aagatgtctt
    15181 taggatatgg tctttgtatg gggaataatg agaataaggc tatatctatg gccatactag
    15241 acaaatcttt ggagccaaat agtgaaaaac tagcacaaga tgaagaattt gtattgcttc
    15301 atatagatgg aatagattca ttaggattta tatctcactt taagcttcct cactatgtag
    15361 attttaaagc ggatgtagaa agaataaaat agtttgtgga ggtaagttta ggtgatatat
    15421 aactatggtt ttttagacga aaacacaaaa aaagaaataa gaagaaagat attaaaagca
    15481 atatcaatac ctggttatca agttcccttt ggttcaagag agttgcctat agctaaggga
    15541 tggggtactg gaggtttaca attaacacta tcattaattg gtaaaaatga tgtagtaaaa
    15601 gtaattgacc aaggaagtga tgattctaca aatgcttgta atataaggag ctttattgca
    15661 agtgtaagta atgtagaaac cacaaaagat acattagaag ctacattgat tcaaacgaga
    15721 catagaatac cagaagaaaa acttaaatct aatcaaatac taatatttca agtaccaata
    15781 ccagaaaccc ttagaatagt agaacctagt gaagtagaaa caagaagaat gcattcagaa
    15841 gaagattata gtaggatgtg ggtatatctt tatgaagata tagttagatt taatgatata
    15901 tctatagcag ttgaatatcc atgtaaagta aatgatagat atttaatgaa tccatcacct
    15961 atacctagat ttgatataaa aaaattaaat atgtcagaca atttattctt gtttggtgca
    16021 ggtagagaaa aaagaattta tgctattcct ccatatacaa aggtagaacc tttagagttt
    16081 gaggattata agtttgaaga ggaaaagttt gaaggaaaat actgtagttt atgtaacagt
    16141 acaaatacat ttttagatga agtatatgac agtgacacta atgaaaagta ctatagttgc
    16201 tcagatacaa gttattgtga aaaagttaga ttaaaaaata acagtataga tgtgacaata
    16261 ggaggtactt ggaatgagta aaataaatgt ctctaaaaga ttagaaaata tagacaagca
    16321 agtattaata ggaagaaata tcaatcttat ttatggagat gggtgtgaaa agtgttttga
    16381 aagcacagga tatgaaagca ataatatatg taagtattgt ggttctatag ttgcatgtaa
    16441 taatgtaaat ttagaacttt atgaaggaga agtccttgga attgtaggtg aatctggttc
    16501 aggaaaaagc actttattaa aaatactgtt tttccaagaa catgcaaata gtggagaagt
    16561 atatatatcc cattacaata aagacacaaa tatactagag ctttcagacc aaaaaaagag
    16621 atacataaag aatcatttta tgggtattgt gtatcaaaat ccccaccttg gtttaaattt
    16681 agactttagt agtggtggta atatagcaga aaaattattg atggcaaatt tatataatgt
    16741 tgaaaaaata agagatagag gcaaagaact tttaataaaa actaatatac ctgtagaaag
    16801 aatagatcat aaaccaaaat actttagtgg gggaatgcaa caacgagttc aaatagcaaa
    16861 agctctttca aataaccctc caatattgct attagatgag gttacaacag gtttagatgt
    16921 ttctgtgcaa gcggaagtat tggatttaat aagagaaatc caaagagaac ttaagatttc
    16981 tatgatagtt gtatcccatg actttgatgt tataaagatg cttgctgata ggacaattgt
    17041 aatgaaaaat ggaagtgtga ttgagtctgg tcttacagac caaataatgg aggacccaca
    17101 acacccatat actcagcaac ttataaattc attgctataa atatcgtttg taaataagca
    17161 aaatgggggt ttttaataaa atgaaagaaa ctatacttga agtaaataat tttagtaaag
    17221 actttgagtt acatgcattg aacaagacta taacggcatg tagtaatata agttttgaag
    17281 tttcaaaagg tgagttttta ggaataatag gtaaatctgg agcaggtaag tctaccatat
    17341 taaaaagtat ctataaaaca tatataccaa caactggaga aataattttt aattcagaaa
    17401 tatatggtaa tgtagacctg gctaaaattg gagaccgaga aatgataaat cttagaaaga
    17461 aagaaatagg atatgtatca cagtttttaa agacacttcc acgaataact gcaattgaac
    17521 tggtagtaca ttctttaatt gagagtgggt ttgaaaaaga ttgttcgtat gatatggcaa
    17581 aagatatact taaccaattt gaaataaagg aaaatttatg ggatgcgtat ccaaataatt
    17641 tttctggtgg agaaaaatta agattaaatt tagctcaagc tatggttaaa aagcctagac
    17701 ttttgttact tgatgaacca actgcatcac ttgataatca atctaaaata tatgtaaaag
    17761 aaaaaatgtt ggaacttaaa agtcaaggaa ctacaatgat aggaattttt catgatatag
    17821 agtttatgga aactgttata gataagacct ttactatggc aaaagggact ataagtgaaa
    17881 gtggggtagc ctagatgata aaactaattc ataatgcaaa tattgtatta gaagatatga
    17941 ttttagaaaa tggtcattta atcatagagg gagatacaat aaaaaaagta tcaaatgata
    18001 ggattgatta cgcatttgac tttaatgaga tagatgaaat aatagatgca aaaaacttat
    18061 atgtaatacc tggtattata gatattcaca gtgatgccat tgaaaaggaa atagagccaa
    18121 gaccaagtac attattacca tttaatatgg cattttatga actagataaa aaactacctg
    18181 caaatggaat aactacagtt tatcattcta tatctttagg ggatggagta ggagttagaa
    18241 gtattgataa ctctttaaag atgattaaaa atattgattc atataaaaat atagatagta
    18301 agagtataaa tcataaagtt catttaagat atgaagtgtt gtattatgaa ggtttagaaa
    18361 aggttttaga actattagat gaaaataaaa tagactactt atcaataatg gaccattctc
    18421 caggacaagg tcagtataca aatccaacat tttataaaga gtatgctact aaagtgtggg
    18481 gagttacaga aaattatgta gatacatggt tagatgattt agtaaatctg catgataatt
    18541 tagattggaa taaaatagct aatattatag gtatagccaa aactaaaaat ataaatgttg
    18601 catcacatga tgatgataca ttagaaaaaa tggatttcat aaaaaatata ggcataaatg
    18661 tatctgaatt tcctataact cttgaagttg caaaacattc aaaaaaattg ggtgttctaa
    18721 cttgtttagg tgcaccgaat attgtaagag gtaaatctca taataacaat ttaaaagcaa
    18781 tggatgctat attggaagat tgttgtgaca ttgtgtgttc ggattatctt ccatcagcta
    18841 tgataaagtc tatgtgtata gttgcagaaa gaataaataa tttaaataaa gcagtcagtt
    18901 tatttacctc aaatcctgct aaagcagttg gtatatatga tgaaagaggt agtataaaag
    18961 aaaataaaaa agcagattta gttttagtag atattgatag tgaatatcca aaggttttaa
    19021 atactattgt aaatggaaaa acagtttata aaagggaggt ataaaaattg ctaacagagt
    19081 tacattgtca tacaagtata tcagataata attttactac tgaagaaata ataaaaaaag
    19141 cagcagaaaa gaatataaat atcttagcaa taacaaatca tgacacatta tctggattgg
    19201 atgaagctct tgtgcttgga gagcattatg gaataacaat aataccaggt atagaaattt
    19261 cagcctatga ttataaaaat agaaaaagag ttcatattct tggatataac atagatttaa
    19321 acagtcaaga aataaaaaat ttgtgcaatc caatggtatg tgacagacat aaagcatctg
    19381 ttaagatggt aaataaaatt atagatttag gatacaaaat atctttggaa gatgtaaaga
    19441 aatattcatc aaagactgga atatttaaac aacatataat gaaagctctt atagataaag
    19501 gatatacaga taatatatat tcaagtctat ataaagaatt gtttcataga ggaaacggca
    19561 aggtatatgt atctttaaaa tatgtggatt acagagatgc aataaaagct ataaaaaatt
    19621 ctggtggaat ttgtgtgtta gctcacccgg gtcaaatgga taattttagc gctattgaag
    19681 agatggtaaa agtaggatta gatggaatag aagtatatca tccatctcac aacaaagaac
    19741 tagaaaaaga gtcgctttct tatgctaaga agtataatct agtggtcaca ggaggctcag
    19801 attaccacgg attttattct caacataatt atgaacttgg aagcaagagc ttaaataaag
    19861 aagatttatt taagtttagg ttagctttag gaggggtatt ataaatgtct tttttacaag
    19921 taaaaaatgt aggtaaaagt tatggacaag ttaaggtgtt aaaagacata tctattgata
    19981 ttgaaaaagg agagtttatt tgtttactag gtccaagtgg ttgtggaaaa agtacactgc
    20041 ttagaataat tgcagggctt gaggataaac atggtggaaa aataataata aatgataagg
    20101 atatgactaa ttcaccacct gaaagtagaa attttggtat agtttttcaa tcatatgcat
    20161 tatttccaaa tatgaatgtt tacaaaaaca tagcctttgg actagaaaat aaaaacataa
    20221 gtaaatctaa tattgataaa aaagttaaag aagttctaga cgttgtagaa ttaagtggat
    20281 atgagaaaaa atatccatca caattatcag gtggacaaca acaaagggtt gcattagctc
    20341 gtgcgattgc tttagagcca gactttttac ttcttgatga acctttatct gcattagatg
    20401 caaaagtaag acttaaactt agggagcaga ttagaagttt acatagaaaa ctaggcatta
    20461 caactataat ggtaacgcat gaccaagaag aagctctttg tttagctgat aagatggttg
    20521 ttatgaatag aggagaaata atacaagttg gaacgccaaa ggaggtatat aaaaatccag
    20581 aaacaccttt tgtagctgat tttataggaa caataaattt tatagatgat ggtattaata
    20641 agatagctat tagaccagaa gatataaaag tagagtctaa tagagattct aaagataagg
    20701 atataaaagt gggagaaatt ttagatatcg aatttagagg atttaactat agaattacag
    20761 ttgagtatcg ttctaagcaa atgaaattag atgtagtttc caaagttgca gaacaaatga
    20821 aactgtgcat aggttctaaa ataaacttta agatacctaa agaaggaatt gtacaatata
    20881 aatcagaggg atgtgcttaa tagctagggg tgaataaatg ataacatcaa aaaataaaag
    20941 aagaataagt gatagcacaa tacaaaaata tttcttgatt ttttcggtaa ttgtactaat
    21001 agtatttata ttatttccat tagtatcatt attaaaaaat gcatttatgg atacaagtgg
    21061 aaattttata ggaatttcaa actttagtaa atatgttgaa aatccaagtc ttatagcatc
    21121 atttaaaaat tcaatttttg tatctacagt ctcatcttta atatcactat gtttagcatt
    21181 tatatattca tacgcaataa caagaacaaa tataaagttt aaagattttt ttaaaactat
    21241 gggaatgttt ccactttttg caccaactat gttatatggt atttcactta tatacctttt
    21301 tggaaataag ggaatcttta caaccatggg tctggaagta ccactatatg gtccattagg
    21361 aataattatt tctcagattc tatttacatt tccacaagct tttttaatat tatctgtagc
    21421 actttcgatg gctgattata gactttatga agcagcagat agcttgggtg ctagtgaatt
    21481 taagaaattt tgcaaaataa caattcctgg tataaagtac tcattactta gttcattctt
    21541 tgtatccttt atacttgcat ttacagattt tggagcagct aaggtagtcg gaggaaacta
    21601 caatgtactg gctactgaca tatataaaca agtagttgga caatttaaca taccaatggg
    21661 agctactgtt tctatggtaa tgttaattcc tgtactaata gcatttacat tagataagat
    21721 ggcaagtaag aaacaaggta tgaccataac tgcaaagtct atgccatata agattaagga
    21781 aaataagttt agagatagag tatttttaat agtatgtagt tttataacat tgctaatttt
    21841 tattttaata ttcgtttcag ttataggttc agttataaag ttatggccat ataatttaag
    21901 ctttacaatg gaacactata catttaaagg tattttggga agtggaataa aaacatactt
    21961 aagcagtttg aaaatatcaa ctttaacagc aatatttgga actatatttg tattttttag
    22021 tgcttatata atagaaaagg tggataagtt tcctaaatta agacaaacag cttacctttt
    22081 atcaatgata ccaatggcct tacctggctt agttttaggt atatcatata taactttttt
    22141 taactcagca agtaatccat taaatttttt atatggaagt acagcaatat tagttatagt
    22201 aaatgtagtt catttttatt cagtttgttt tataacctca aattcatctt taaaaatgtt
    22261 ggataaagag tatgaattag ttgcaaaatc tataaatatt ccattctata aagtattttt
    22321 taatataaca gttccgatgt ctataacatc aatacttgaa atagtagtat attattttgt
    22381 aaattcaatg gttactgttt cagcattaat atttttatat atacctcaaa ctcagactgc
    22441 atcaatatca atactaaagt tagatgagat aggatatata ggaccatcag ctgctatggc
    22501 agtattagtg ctattaacaa atataatagt aagacttttg tacgaatttg taacaaagaa
    22561 attaaaaaat aacacacaaa aatggcagca aaaagatgtt gcatagggga ggaaaaaatg
    22621 aaatttaaaa agctagtttc tttaggagta ttatcaacac ttttattaag tgtaataaca
    22681 ggatgtagta ctgattcatc aggttcagat tcaaagtctg atagtaagac aaaaggggat
    22741 ttaacagttt acacagcaat tgaagaagat agtatagagc catatttagc tacatttaaa
    22801 gagaaatatc cagatattaa attaaatatt gttagggcat caacaggaga tataacagca
    22861 agattattag cagaaaagga taatcctcaa gcagatgttg tatggggagt tgcagccaca
    22921 agcttattgg ttgcagatga ccaggggatg ttagaaccat atgctccaaa aggttctgaa
    22981 gaaatagagt ctgcatttaa agatgataaa gaagttcctt cttgggttgg gatagatgct
    23041 tggatgacag gtataacagt aaacactaaa gaattaagtg ataagaacat cccagttcct
    23101 agttcttacg aagatttaat aaagccagaa tataaaggtc ttatatctat gcctaatcca
    23161 tcttcttctg gtacagggta cttaacagta tcagccctta tacaaattat gggggaagaa
    23221 aaagcatggc agtatatgga taagttgcat gagaatattg gtgtatacac tcaatctggt
    23281 tcagctccag ctacatcagc agcttcagga gaatatccta ttggtatatc ttttggatat
    23341 agaggtataa aattaaaaga ggaaggctat ccagtagaag tagtatttcc aaaagaaggt
    23401 tctggatggg atatagaagc aaatgctctt gtgaaaaaag ataatataaa agaagcatca
    23461 aaagtatttt tagattgggc tataagtaaa gatgcaatga atgaatactc taagaactat
    23521 gcagttacta ctatttctac aggaaatcca ataccagaag gattccctaa aaagccatta
    23581 gagcaaatga tagataatga tttaaaatca gcagcaaaaa atagagaaga tatactaaat
    23641 aaatggatat caaaatatga tggtaagaca gaaaaagaga gttaagtaaa actatacaaa
    23701 tggagtaaaa agaaaaaatt tttttaagta gattttatta taataatata agttgagaat
    23761 aatataggtc aagaaaagtg aatattctat aaaaatgtat agagtaagta taccattcag
    23821 tttgaatggt atatttttta tttttagaga ttaatttaga aatagatata ctacagttta
    23881 aaatagtata ttttaatttc attattaaag tatagatgtt atatataaag taaataagat
    23941 attaaataat tataaactaa agtaaataaa atattgagcg attattaaaa agatatatat
    24001 atatatttta atttgagcag tttgttatat attataaaaa aagagaaaaa taaaaggtaa
    24061 attgtttctg gataacttaa aattggaagt aagaatattt aactagagaa gaattattta
    24121 agttcaggtt agctctagga gtagctgatt ttataggagc aatagatttt atggatgatg
    24181 atataaacat gatagcaatt agaccagaag atataagggt agaatttaat agagatttca
    24241 aagaaattat agacctcaaa ctcagaccgt atcgatataa aaactaaagc tagatgatat
    24301 agtgttgtat ataggaccat cagctgttat ggaagtatta gtgcttttaa caaatataat
    24361 agtaaaactt ttgtatgaat tgataataaa aaaattaaaa accaagacat aaaaatggca
    24421 acaaaaagaa gttgaataag ggaggtaaaa atgaaattta agaagttagt ctctttggga
    24481 atattatcaa cacttttatt gagtgtaata actggatgta gtaatgtttc atcaggttca
    24541 aacttaaagt ctaataataa gacaaaagga gagttaacag tttatacagc aattgaggaa
    24601 gatagtatag atacatactt agctacattt aaagagaaat atccagatat taaattgaat
    24661 gtacttagat tatcagtagg aggtataaca gcaagattat tagcagaaaa agataatcct
    24721 aaagcagatg tgttatgggg tgttgcagct acaagtttga taattttaga tgacaaagaa
    24781 atgctagagc catacgctcc gaaaggcttt gataaggtaa atcctgcatt taaagatgat
    24841 aagaaagttc cttcttgggt tggaatagat gcttggatga ctggtataac agtaaatact
    24901 aaagaattaa aagctaaaaa catatcagct cctaattctt atgaagattt aataaagcca
    24961 gaatataaag atcttatatc tatgcctagt ccagcttctt caggaacagg atatttaaca
    25021 gtatcagaac tcatacaaat tatgggtgaa gaaaaagcat ggcagtatat ggataaattg
    25081 cataaaaata ttggtgtata cactcagtct ggttcagcgc cagctatatt agcagcttca
    25141 ggagaatatc ccattggtat atcttttgga aatagaggta taaagttaaa aggtgaaggt
    25201 tatccagtag aagtagtatt tccaaaagaa ggttctggat gggagataga atcaaatgca
    25261 cttgtgaaaa aagataatat aaaagaagca tcaaaagtat ttttagattg ggctataagt
    25321 aaagatgcaa tgaatgagta ttctaagaat tttgcagtta ctacaattga cacgggaaat
    25381 ccggtgccag aaggatttcc aaagaatcca ttagaacaga tgatagataa tgatttaaaa
    25441 tcagcagcaa gaaatagaga ggctatacta aataaatgga tagaaaaata tgatggaaag
    25501 acagaaaaag agagctaagc cttacaataa aatttagtac aaaaagcgcc tagaatataa
    25561 gttttacatc taggctcttt tgatatattt atttaaaatg catatttcta tataaaaata
    25621 aatagtttag taccttgatt aaattaaata aaaaccttaa aataaagaga tgtaaagaaa
    25681 aactaaaaac gaataaaagg tgaatttata tgataaaaga aataatagta gtagagggta
    25741 gagatgacgt tacagcagtt aagagagcaa tagatgcaga acttataact acaggtggat
    25801 ttggatttcc aaaaggtgta atgcagcgta taaaagctgc taatgaaaga cgtggtgtta
    25861 taatatttac agacccagat tttgcaggtg aaaaaataag aaaaaaaata gcatcagaag
    25921 ttccaggatg taaacatgca tttctaccaa gagaggaagc taaaaaggat ggagatatag
    25981 gtattgagaa tgctactcct caaagtataa tagcagctct gaataaagtt agaactgaaa
    26041 atacagaaaa aagagatgaa tttaagcaag ttgacttaat tagaaatggt cttataggta
    26101 atgaagaagc atcacttaga agagatgcac ttggaaaaat acttggaatt ggatatggaa
    26161 atgcaaaaca atttttaaat agattgaata attatggagt tgaaagagaa gaatttatag
    26221 atgcattaaa acaaatctaa acgtaatgtt taatgtatga aaaatatgta tataaaataa
    26281 attgtggaat tacggttaat ttaagataaa ttatttataa tatatacctt attttaaatc
    26341 aatgtaaata catataaata agtataatta ataaaattaa atatcaaaat aagttaaaaa
    26401 ggagtaaata atggatagac tatcttcaca taatgcaaca aaggaagtag tacaaaaata
    26461 taattttaaa ttttcaaagt ctttaggtca aaatttcttg atagatagta atataattga
    26521 taaaatctta tctggagcta gaatcacaag aggagataat atcatagaag taggacctgg
    26581 tataggaact cttactcgtg agatgggtaa aatagcagaa aaagtagtgg ctatagaaat
    26641 cgatagaaat ttaattccta tattgaaaga taccttgtca gatttagata atacagaggt
    26701 tgtaaatcaa gatatactaa aagtagacat acaggaatta gtaaaagata aattaaatgg
    26761 tggaccagtt aagcttgtag caaatcttcc atattatata actactccaa tagttatgaa
    26821 atttttagag gaagacatac ctgtaacaga catagtagtc atggttcaaa aagaagttgc
    26881 agatagaatg aatgctattc cgggtactaa agattatggg gcattatcta tagctgttca
    26941 atactattgt gatacagaga ttgtggcaaa agctccaaga catatgttta taccacaacc
    27001 aaatgtagat tccacagtta taggtttaca tgtaagagat aaaagaaaat atgatgttca
    27061 taatgaagac atatttttta aaacagtaaa agcatccttt ggacaaagaa gaaaaactct
    27121 tcttaattcg ttgggaggtc ttggttttct taataaagat gaaataagag aaatattaaa
    27181 agaagctaat atagacgaaa aaagaagggg tgaaactctt agtatagaag agttttcagt
    27241 tctttcaaat ataataaata caaaagtatc ttctaaatag aaggtacttt ttttaatttt
    27301 gttaacatat attgtttaga gaaactgtgt taagggggga ttgcagtggg ttctaaaaga
    27361 aagtttaaaa gagactatat aatactagaa gctaaagaca taaactttag atacaaagaa
    27421 cgagtactgc caaaagcttt tgcaaaagtt gaagtaaatg atgaaaaagc aattattgca
    27481 ttatatgtag aaaatctaaa atatgttaaa gatgggtata aagcagtagc aataaaagat
    27541 gaccatggat taatagactt aggaaatata attttaaatg aacaaggaag aggtgaattt
    27601 gttttagatt tagaagattc tgatgatgag ataaaaggta ttgcattatt gtatgaaaga
    27661 aatatacctt taattggatt taagggaaat aagcttgaaa attatgagga catattattt
    27721 caagaggtag aggaagaaga atatgaagaa tatgaagaat atgaagagtt ggaagaagac
    27781 gaagagatag aggataatga ggaaagtgat agagacgaag atagtgaaga agaatatatt
    27841 atagaagaat atgatgaaga tgagtatgaa gactattggg aagatgatga agatattgaa
    27901 gaaggagagt taaaagcaag aacggaagta gaagattctg gtgatgagga aatagaagag
    27961 ttcaataatt ataaggaaat agaaaattat aatagggtta aagtacagga agaatatata
    28021 gacatagaat atgatgagga agaagaggaa gaagaagaag tttttgaagt aaatagagaa
    28081 gaagataggt ttgtttcctc aaaacctact aaatcaaaga aagttcaaaa agatcagaat
    28141 aatattgaaa aaataaaaag agctaatgga gtaagtcaag gagagagtgg aaataaacaa
    28201 accagtacat tacttatgcc aagacaaata aaaaaaggtt tgaaattatt taaagaagta
    28261 aaaccttttg taacagatta tatagataag acaagatggt ggaaaataga gataaatcct
    28321 acaacactgt gtggatatac aatgccttat ttaggatatt taaacgtatt gaattacact
    28381 atgtatagtg atgcagtttt acattcatat aaatacagac actatttatt tggtgttcag
    28441 tatgatgaat ataataagag aaaacattat atttatgcta tcccaggaaa tagaaatgaa
    28501 cagccagaca atggaagcac aggattttta agatatcaag cttgtgataa tagaacaaat
    28561 agtttaggat attggctatg ttttatagat tctagacaaa gaaatatagt taggtaaaaa
    28621 taataaaaat gaaaaaattt agactcaatt cttattttac tgaaaaaatt aatgaaatgt
    28681 agtacaaaac tttaaaaact attgataata tttaaatatt acactacaat atatcttgaa
    28741 atacctatta tattatggag gaaaattatg ataaacgagc aaagaatttt aaatgagttt
    28801 cttgaattag tacagataga tagtttatct ttaaaagagg ggaatgttgc taaagtatta
    28861 gttaaaaaat tagaagaaat aggttgttca gtagtaatag acaatgctgg tgaaaaagca
    28921 aatggagaaa ctggtaatat aatagctaca ttaaaaggaa ataaagaagg aaagaaaata
    28981 ttatttagtt cacatatgga tacagtaact ccaggtatag gggtaaaacc tatagtggat
    29041 gaagcaaatg gaataataaa aagtgatggt actacaatat taggttctga tgataaagca
    29101 ggtatagcag ctatattaga aggcttaaga tatataaatg aaaataacat agaacatact
    29161 gatatacaag ttgtattctc tatatgtgag gaatgtggat tagttggagc taaaaattta
    29221 gactatggaa aaatagattc tgaatatgca tttatattag atagtggtgg ttctccaggt
    29281 gaaataatag taaaagctcc tgcacaagat gtaataaatg ttaagatatt aggaaagact
    29341 gctcatgcag gtcttgaacc tgaagctgga ataagtgcta taatggtagc tgccagagca
    29401 atagaaaata tgaatttact tagaatagac gaagaaacaa ctgctaacat aggaattata
    29461 aacggtggaa cagctacaaa tatagtaaca ggagaagtta atatagttgc agaagccaga
    29521 agtctaaaag aaaataaatt agatgttcaa actaaacaca tggtagagac ttttgaaaaa
    29581 gctgcaaaag attttggagc tcaaattgag atagatgtca atagagcata tacaccaata
    29641 gatgttagtg aggattctga aataataaaa cttgcaaaga aggctttttc aaacttaggt
    29701 atagaaggtc atactgaatc tacaggagga ggtagcgata caaatatatt aagtaagaat
    29761 ggtatcgaag ctataacttt aggaattggt atgaaaaatg ctcatacact aagtgaacat
    29821 atagctataa aagacttata tgactctgct aaaatggtag tggagataat aaaggaagca
    29881 taaatttaaa taatagtgta taatatatat atcgcctagc ttaagctagg tgatttttca
    29941 ttttatagaa gattgataat ctctattgaa agcaaagagt atacatttgc atataagaga
    30001 gtgtttaatt tatgttcaga tgtaggttct taataatatt tagattgttt cataagggag
    30061 gaataaatat ggaaacaagg tatgaagaag caaataaaat aacaatacaa tcaatacttt
    30121 ggaatgtagt attaacaata attaaggtta tagctggtgt tattggtaat tctagcgcta
    30181 tgattgcaga tggattacat tcagcatcag atattataag ttctatagga gtactaatag
    30241 gcaattatgt atcttctaga cctggagata gagagcacaa ttatggtcat gagaaagcag
    30301 agacattagt atcatttgtg ttatcaatat tgcttatttt cgtttctata acaataggta
    30361 tagaggcaat taaatcttta tttaacttag atgcattaag tgttccatca atattaccat
    30421 tagtagtatc agttatttca atcctgataa aagagtatca atatagaata acaataaaag
    30481 ttgctaaaaa gataaattca ccagccttaa aagctgatgc ttggcatcat aggtcagatg
    30541 ctttatcttc agtagctgct tttataggta taggtggttc tattttaggg tttaagccac
    30601 tagacccaat tgcgtcaatt gtagtggcta tatttgtagc taaagtagga attagtatac
    30661 ttataagttc tgttaatgaa ttgatggatg tttcagttga cgaagaagag ataaaagaat
    30721 taaaatttat tgtagcagat acagaaggtg ttaaaaatct tggtgatata aagactagaa
    30781 aacatggagc tatggcatat gtagatttga ctatatgtgt ggatgaaaat ttgacagtta
    30841 agcaaggtca tgatattgct accaagcttg aaaaacatat aataaaacat atggaatttg
    30901 taaaaggcat aacagttcac gttgaaccat gtactaattg ccaaggaaat aagtgtaata
    30961 attaattaaa acttaaaaaa aagtgtaaaa agttatataa ttgttttatg attataaact
    31021 ctaggggtgg ttaaatgagc aagaaaagaa aactaaagaa aaaagctgca atattaatag
    31081 gagtagttgt tttctttata atatacttta tatcctttag aataggaata aatatggaaa
    31141 aggataaaag tgaagtgcca aagaaggtag aaaaagaaga agaagataat aggtctttaa
    31201 ggactcaaat aaaagaaaag aaaaaggttt atatatcaga caaaaatata gaaaacataa
    31261 aaattgaaga agatatgtgg gatgaaataa agttattttt ctcagatttt aagaaagtaa
    31321 gaaatactac ttcttcttac gaagcagttt atgaaggtta ttctgatgat ggagttaaat
    31381 tttctactga ccttaacttt tttagagtat atactgtaaa taaagaagaa tattataaag
    31441 taccagttag ttctaaggat gcttttaaga aacttttaga tgagagtata tatacttcat
    31501 ttgacctttt aagtcattat aaagattgga aagaggttac tataactcat ggaaaagaaa
    31561 ttaaaaaagt tcatagatgg aaatttgatg atttagcaca taagatgatt tcaaagaggg
    31621 ttgtaggaaa agttcaacct gagaaaagta aggaaagaag tgactataat tttactataa
    31681 atataaaagg tgataatttt gatgtaaaag ttgatacaat gggaaaagat tatgtaaaag
    31741 ttacttctaa gaaaaatgta tcttactatg aggttcatac aagtttattt gattacctta
    31801 aaaatgagat atttgaaatt ggaaagaaat aagactttaa gaaggctatc aaattagata
    31861 tttgatagcc taaattattt ttatatatta tttttcaacc aacaagtctg caaccttata
    31921 aattggacca gcaccagcag ttattactaa atcattatcc ttaacatttt cacgtaagta
    31981 ttttactata tcttcaaatt cttttatata gatggcatct acattgtttt gatataattt
    32041 ctcaaccaag tccttagaat gtatatctcc agggtctttt tccctagcag catatatatc
    32101 tgttatgata actttatcag cagcatagaa tgcctctgaa aattcaccta aaagagattt
    32161 tgttctagta taagtatgtg gctggaagat acaccataaa gttgattttt ttaatttttt
    32221 ggcagcagct aaagttgctt ttaactcagt tggatgatgt gcataatcat ctactactaa
    32281 agcattctta taataccctt tagtttcaaa tcttcttccg acacctttat aaatttttat
    32341 attttttctt atagtctcta agtcaatacc agaaacaaga gctgccatta tagcagaaga
    32401 agcattgtat atgttatgaa gaccatatac agaaagttca aattctccta aatctcttcc
    32461 ttcatattct attctaaata ttccatgacc actattgtca aagtgaatat cttttattac
    32521 agcatcattg tcagaatctc taccgtattt tataatagta gctttaacat catacaatat
    32581 gtcatcaata ttttcatcat cgccatttat tatgaaatag ccttctgggg gtaataattt
    32641 tccaaatttg ttaaaagaag ccttgatttc atctattcca gagaaataat caagatggtc
    32701 ttcttcaaca tttaaaacta tagatatctt tgggttaaaa ttaaggaaac tatctacata
    32761 ttcacatgct tcagttataa agtggttgga attacctatc ttaacattac cacctatcat
    32821 acttaagtta ccaccaacta gtatagttgg gtctaagtct gcatattcaa agattgtaga
    32881 taacatagat gttgtagaag tttttccatg tgttccagaa actgctatag aatttttata
    32941 ttccctcatg atttggccta aaaaagtcgc tctgttcatt accaatttat ttttttcttt
    33001 agcagccaca agttcttcat tgtctggatg aactgctgct gtataaacaa ccatatttac
    33061 atcatctgat atattatctt ttttctgacc aatataaata gttgcccctt ggtccctaag
    33121 tttgtctaat aggtatgatt cgtttgaatc cgaacctgaa acttgataac ctttattgat
    33181 acatatttca gctaatgcac tcatgctgat accgccaatt cctataaagt gtatattcat
    33241 aataaaacca cctttctttt taattaagtc ctacaatatg aactatttat gtggtataat
    33301 aatataatgt tcaatatttt ataattaata tgcatataat attatatgta ttttattttt
    33361 gaatataata aaacataagt aatattatac cacaataaga attgttatgc attagttagt
    33421 ttaacgttat ataaaggtac ttgtaggagg aaataataat gaaatttaaa agaacagaga
    33481 gaattggagc aatagttaaa atactgtctg ataatccaaa taaaatatat acattaagtt
    33541 attttacaaa tcaatttaat gcagcaaaat caacaataag tgaagattta ttagttgtaa
    33601 aaaatgtatt tgagaagcta catttaggta aagttataac tatctcagga gcagcaggag
    33661 gagtaaaata tatacctaaa acttctatag cagaaaatgc agagttctta atggaattat
    33721 gtgagagaat atgtgacaag tctagaatac tatctggtgg ttttttatac ttgattgacc
    33781 taatttatga ccctacaata gctgctaaga taggaaagat attcgcatca aatattgagt
    33841 atgtagatgc agattatgtt gtgacaatgg aaacaaaagg tatacctatg gcacttatga
    33901 ctgcaaaagc tatgaattta cctctagtta taataagaaa agatataaag gtatcagaag
    33961 gtcctacact tagcatgaca tatgtgagtg gaaacagctc taaagttgaa agtatgagtt
    34021 tgccaagaaa agctcttaaa ccagatagta aagttataat tatagatgat ttcatgaggg
    34081 gtggaggtac tataaaagga atggtagacc ttatgaatga atttggtgca gaggttatag
    34141 gtactggagt attcatatct acaacgaatc cttcagaaaa aatggtaaaa gattatattt
    34201 cattgattca attggatgtt aatggagata aaatagtagt agaaccaaac ttaaagactt
    34261 ttaaagatga atatagaaat gaagatttag acgaagaaga agattcagaa tttgaatttg
    34321 agatagatga agattaataa aaaaaatatt tagaaaaata tgtaaaaata aaaagaggat
    34381 atccctagtt gttcatagaa ttatttaaaa aacagtttat attaatttat tccagcgata
    34441 ataattttaa tataaattaa tttctgtcaa aatcaaaggg ggatattttg gtatgaagat
    34501 aactgacgta agagtaagaa aattaacaga agaaggtaag atgaaatgta tagtttcaat
    34561 aactttcgat aatttgtttg tagtgcatga tataaaagtt atagaaggcc ataatgggct
    34621 atttatagca atgcctagca gaaaagtagg cgaaggaaat ttcagagata tagctcatcc
    34681 aataaatgca gaaatgagac aagttttaga agatgcagtt ttacaagcat atcatgaagc
    34741 attagttcaa tgggaagtag ctgctgaata ataaatatat aaaatggaga gagcccgaac
    34801 atttaggctc tttttaaatg tagaatttct attaaaatat gtacttttta gtaaaaaagt
    34861 gctatattat tagtgataat gtatatgttg tatacaaatt gttagataaa tgtattataa
    34921 tgtatattga ttaagagaat aatacgaata aaattaaatt agattgaatg gagtgtatca
    34981 atgaacttta aagctataat ccttgctgct ggcaaaggaa caagaatgaa gtctaaatac
    35041 ccaaaagtta tacataaagt atgtggaaaa gaaatggtta accatattat agatgtttca
    35101 aaaaaatcag gggtaaaaga tactgtcgtg attttaggac atgaagctga ggtagttaaa
    35161 gaaaagcttg cagaagaaat tataatagcc atgcaaacag aacaacttgg gacaggacat
    35221 gctgtaaaga tggctaaaga atatattaat gatgaagata caatagttgt actttgtgga
    35281 gatactccac ttataaaaga agagacactg aagagattat ttgaatatca tatagaaaat
    35341 aaatatcatg ctacagttct tacaacaaga gttggaaatc caacaggtta tggtagaata
    35401 ataagagata agaagggtga ccttcttaaa atagtggaac aaaaagatgc aaattcagaa
    35461 gaaaagatga taagtgagat aaactctgga atttattgtt ttaatggaaa gagtcttaga
    35521 gaagcactag atttgctaaa taataacaat tcacaaggtg aatattattt aactgatact
    35581 gctaaaataa tgagagataa aggattaaaa gttggtgcat ttgcaggttc aactattgaa
    35641 gaacttatgg gcgttaattc aagagtagaa ttatcaaagg cagaagaaat tatgagacga
    35701 agaataaatg aatctcatat ggtaaatgga gtaactataa tagacactaa ctctacctat
    35761 atagaatctg atgttatgat aggcaatgat actataatat atccaggtgt tatgttacaa
    35821 gggaaaacaa ggataggttc agattgtata attggaatga actcatctat aacaaattca
    35881 gaaataggtg atggtacaga aataaaaaat tcaactataa ttgatagtaa ggttggagaa
    35941 aattctaatg ttgggccata tgcatactta agaccaaaga gtgacttagg aaataatgta
    36001 aaaataggcg attttgtaga agtaaaaaat gctattatag aagatggatc aaaagcatct
    36061 cacctttctt atataggaga tgcacatgtc ggtaaaaatg taaatattgg atgtggagta
    36121 gtatttgtaa actatgatgg taaaaataaa tttaaatcag tagtaaaaga caatgcgttt
    36181 ataggttcaa attctaattt agttgcacca gtagttgtag aagaaaaagg atatatagca
    36241 acaggttcaa caataacaca tgatgtccca gatggtgctt tggcaatagc aagagaaaga
    36301 caagttatca aagaaggatg ggtagaaaaa aagaatcaaa aagacgacca aagtaagtaa
    36361 ttatgtagtt tattattttg gctaaaaaat ttagctcgaa attattataa attatattca
    36421 ttagtatttt aaaattttca ggagggttac ataaatgaat actagcggaa gcgagattaa
    36481 aattattgca ggtaattcat cgaaagagtt agcacaaaaa atagctgact atataggtgt
    36541 atcagtacta gattgtgaag taggtacatt tagtgatggt gaaatatgtg taaacatgaa
    36601 tgagacagta agaggttgtg atgtgtttgt agtgcaatct actaacagcc cagtaaatga
    36661 taacttaatg gagttattaa tcttaattga tgcgttaaag agagcatcag caggtagaat
    36721 aacagcagtc attccttact atggatatgc aagacaagac agaaaggcta aggcaagaga
    36781 tccaatcact gctaaattag ttgcaaattt aataactgct gctggagcag acagagtatt
    36841 aacaatggat ttacatgcag ctcaaataca aggatatttt gatataccat tagaccactt
    36901 actaggtgga acaatattag caaattattt taatgaaaag aaaatagaag atttggtagt
    36961 agtatctcct gacttaggaa gtgttactag atcaagaaaa tttgctaata ctttaaatgg
    37021 agaagttcca atagctataa tagataaaag aagaccgaaa gctaatgtat gcgaagttat
    37081 gaacttaata ggtgatgtta aaggtaaaaa tgttatacta ttagacgata tgatagatac
    37141 agcaggaaca atagttaatg ctgcaaatgc acttaaagag tttggagcaa aagatgttta
    37201 tgcttgttgt actcatggtg tgttatcagg tccagctatt gaaagaattg ctaattcaga
    37261 aataagtgag ttaatagttc ttgatactat acaacttcct gaagagaaaa gaatagataa
    37321 gataaagata aaaacagtag ctcctttatt tggagatgct ataagaatga tattctctaa
    37381 tgaatcagta agtaaattat tctaatttat tgtatttatg agagttgttt aaaaggatat
    37441 aaaccttttg agcaactctt ttttgatttt atacttatgg aatgttattt ttttcttgtt
    37501 agaaaggtat atattattgt aaaaagagat tatagtttaa aattatgtat atttaagtaa
    37561 aataaactta ataaagggca tattattcaa aatattatga atataaaaaa tattacaaaa
    37621 aagtattgaa aaaattcaga ttatgtagta ttatgtaaat ataaataaag aaagaggtgt
    37681 ttccaatggg aaggttagaa gatgaacccc taataattaa atgattcagt cacaatctaa
    37741 agtgaatata aaaatctatt gaaagtttat ttataagaat tgaaaatagt atctattcta
    37801 tctttagtgt ttgtgagagg gttctggatt atataagatt actgtaaaga gtaattttgc
    37861 acaataagca tagctgtaaa tgaagagttt cgtttatata atgattgtgg ctgaatcata
    37921 actataatag atagaatgtt tataatagat gattttataa aatactatat tgtaatagct
    37981 tttagctttc aaataaatat tagcaaagga tggaaatgac aatgataaaa ctaaatgata
    38041 atttataaat atctccttac gatatataat aattgtaggg agatgtttct tttttttgta
    38101 gagtataatt tttctagaaa ttctgtttca atatgtaaaa agttggtata tataaatagt
    38161 ataaacagtg ttgctaaaat atggtagata ttatataata taagtggata tgtatataca
    38221 ttatgagtta tataattttg aatttaataa aatagaaacg ttgatttatg ttctgtaatg
    38281 tgggcacctt agagcatatt gagttagtgg tgcaaccggc tatgaaattg tatttattta
    38341 tagatactat cattttcata gcttttttta tttaaaaaat ggttatgtgt tagaaataaa
    38401 tttggttagt acgtttaaat aattttactt agatttttaa ccagatatat tataagtaag
    38461 tattaattaa aaaatttaag ggggaataaa aatgaagtta aaaaagaata aaaaaggttt
    38521 cactttagtg gaattattgg tagtaattgc aattataggt atattagcag tagtggcagt
    38581 tccagcttta tttagtaata taaacaaggc taaggtagca agtgttgagt ctgattatag
    38641 ttcagttaag agtgcagcat tatcttatta ttcagatact aataagatac cagttacacc
    38701 agatggtcaa actggtttaa gtgttttaga gacttatatg gagtctctgc ctgataaagc
    38761 tgatataggt ggaaaatata aattgattaa agttggtaat aaattggtat tacagatagg
    38821 tacaaatgat gaaggagtta cattaacaga agcacaatca gcaaaattat tgagtgatat
    38881 aggtgaaaat aaaatatata caagtgttac agcagataat ttgggaaatc cattaacaag
    38941 taatacaaaa gtagataata aagttctata tatagtactt atagataata ctgtgatgga
    39001 ctcaacaaaa tagttaaaac tatattttta taaaaatatt gtaaatttaa tgaaaaatat
    39061 aatataaaag acaggaagaa aatcttcctg tttttttata ctattttctt taatgttcaa
    39121 ttatgaatga tgatataatt gagatagtaa aggagtgaaa cctgtggcta aaaaagttag
    39181 aataggagac aaactggtcg aaaaaggata tataacagaa gaccagctta aatgggcact
    39241 atcagaacaa aaaaacagtg gaaaaagact aggagaattt ctagtacaag aagggcttat
    39301 agatagtaac ttactaataa gtgtattaaa agaactcttg gatatagaaa gtatattttt
    39361 agaagggaca gaaatagaca ctttagctac aaagatggtt ccagaaaata tatgcaaaag
    39421 atatactgta tttccattta aaattgatgg aaataaaata tgtctagcca tgtcagaccc
    39481 acaagacaga gaagcagttc aagatgtaag gcgtatgtct ggtaaagacg tagagatatt
    39541 tatatcaagt acagaagata taaacaaagc aataggtcat gcttatgcac attcagaaat
    39601 aaataaggca atgactgagt acaacaaaaa tagaactggt ggagtaagag aaacagtaat
    39661 acttgaagaa gatgtaaatg ctgctccaat agtacgatta gtaaataaca tactagaaaa
    39721 tgctgttaga atggaagcaa gtgatataca tatagaacaa agtgaaaatt acatgcgtgt
    39781 taggtttagg atagatggaa tgttaagaga atatatgagg atgaactcag caccatataa
    39841 ggcagtaatc agccgtatca agataatgtc agatataaat atatctgaaa agagaatacc
    39901 acaggatggt agaatctatt taaaagtaga taataaaccc atagatttta gggtttctac
    39961 aatgccaact aacagggatg aaaagatagc tatgagggtt cttgataaga gtaattttat
    40021 ggttagtaaa gaagtactag gaatagacga acatggctct aagatatatg atgaattgat
    40081 aaacactcct tatggtctta tattggttgt tggtcctact ggaagtggga aaaccactac
    40141 attgtattca atgttaaatc aactaaatac agaaaataga aatctattaa caatagaaga
    40201 ccctatagag tatgaattac ctggtgtaaa tcaatctcaa ataaatgaaa aggcagggct
    40261 tactttcgcc agcggactta gagcttttat gcgtcaagac ccagatataa taatggttgg
    40321 agagataaga gatactgaga cagcagaaat agcaattaga gcttctctta caggacattt
    40381 agttttaagt acattacatg caaatacagc agttggagca atttcacgtc ttttagatat
    40441 ggatgtggaa agttttttaa taacttcatc tgttttaggt gtaatatctc aaagacttac
    40501 acgaaaaata tgtgagcatt gtaaggtttc atatgaagca gatattggag aaaaaaaggc
    40561 tcttggtatt gatgtaaatg aatcagtaac tatttataga ggaaaaggtt gtgaaaggtg
    40621 taataatact ggatataaag gacgtctagg tatatttgaa atgcttgaaa ttactccaga
    40681 aataaaagag ttaatagatt catctgcaaa tcaaagagaa attttaaaga tggctagaaa
    40741 acaaggcatg gttagcttaa aagaggatat agttaaaaag gttttaaatg gaaagacaac
    40801 agtagaagaa atgattagaa taattttgat gactgattaa aggatgatat tatgaaaagt
    40861 tttaaataca cagtaataaa agaggacgga aaaaagcaaa ctgatgtgat tgaagccaac
    40921 gattttaatg aagctagaaa tcttcttaga aagagaaatc ttagagttat tgaaatcaaa
    40981 gaaagcaaaa ataaaaatat aagattatct agtagaaaga gaaaaaaaga cttgggagct
    41041 gaccagataa gccacttttg tagacagttt gctattatag tatcttctgg tataaatagt
    41101 atttcaggat tagaaacatt agctagaagg tcaactaaca ttacattaag agaagaaatt
    41161 aatcgtatag tggcagaaat aaaagtaggt tctactattg cagattcaat gctttcatca
    41221 aaatcaaagt ttccaaagtt acttggtgct atggtagcta caggtgaagc tacaggtaaa
    41281 ttagatgaag tattaaaaag tatggcttca ttttatgcaa gtgaacacag ggttaaacaa
    41341 aagcttagaa atgctgcaac atatccatta atagttttga tttcttcatt tataatgatt
    41401 tttatattta caacatttat ggtgcctaag atgattaatt caattacaac agtaggagca
    41461 actcttccac ttataacaag aattgttatg gggtttggta tgtttatgaa gaagttttgg
    41521 ataatagtac ttttagttat tatattattt atctatcagt ttaaaaaata tttaaaaacg
    41581 tcagttggaa gggctcataa agatagagtt ataaacaaga tacctgtttt aggaaaagga
    41641 ataaattgta tggtagctac acgattttca agagcattgt atctatttgt atcaacagga
    41701 tacccattag ttcaagggct tgattatatt atagatagtg taaataacac catggcagag
    41761 aagattttag catcagctaa agatggaata acaagaggag aaggcttagc agaaaattta
    41821 gagagatata cgtattttga ctctgtgttg gtacagatga tagctatagg ggagcagaca
    41881 ggggaattag agaatataag tagacagatg gcagaatttt atgagtatga atccgagatt
    41941 tatttaaata ggatggcctc aatgatagag cctgttttaa taattgcagt tggtattata
    42001 gttgcgatac tggttgtatc aatatttatg ccaatgctta gtatatatga tgccatgtag
    42061 gagggaaagg acaaatgaat aaggtttatg tatcatatta tggcgattct atttccatag
    42121 ttgagggcag atataaacac gataaggata agttttatgt aaaaaatttt aatgtgtttt
    42181 caatagagga tattgcacaa gatttttcta aggaaaagta tgctctattg agatacgcat
    42241 tagaaaatgt taagttaaaa tcaagacatg tagttttttg tttaaataca agagatgtaa
    42301 ttttaaaacc ccaaaaactt cctaatatgg acaaaaagga tttagatgga tttatgaagc
    42361 tagagatgga cgaaatgatg gctttggagt cagatgaata tgtattttcg tatgaagtat
    42421 ctagtgaaac acatgataat tcagatggcg atgaatcaag cttaaatctt atcattggag
    42481 ctatacaaaa ggaagaggta aatgcaattg tagagataat gcatgaattt gatttagtat
    42541 tagatagaat agatacatta tcaacagctt acttaagaat gcttaaaaat ttagactaca
    42601 atgatataat ggtaaccaat ataagtgaca atagtacaat tataaatata tataagaaag
    42661 atactttatt tattagtgat aatataccta taaagataac tgctaaagat atggacatgc
    42721 aattattaaa tgtagtagaa gaatcaaaag gtcttatgaa ttactattcg tcaagaaact
    42781 ttggtaaggt tacggatact atattaatat taggaaaaag atattccaat aaattaattt
    42841 atgaaacttt tagctctgca ttttcaagct atgtttctca aggccttact gagataatgg
    42901 atatatctga tatgataact ggagatatag atgaagatga tataaattca attgtagagc
    42961 ttttaggaag tatgatagaa cctaaaggga aaagagattt tgaaaatata aactttttac
    43021 caatggatat acttagaaaa cagataaaag ctaaacaaat gaaaaacata ctgaaaattg
    43081 ctccaatagt tattttgatt cttgctatac cttatttatg tttaattttt tcaaataatt
    43141 tagcaaataa ggaacttgaa aatgttaatg gtgaaataag aaatattgaa tctgcatatt
    43201 atcagattgg aaatatagaa aaaatgatac agagtaaaag cgaagaaata aaggtatatg
    43261 atatgttaat tgggaaaaag gtaaagtggg aaaatttatt ggatgatata gataaaaata
    43321 ctccaagtaa agttgaacta acaagtttat caacaactta tcaaaatctt ggaactaata
    43381 ataaagctga taattcaaaa agtaataaga ctacaacaga taaatcaagt aacaatgaaa
    43441 caaaatcaag tacgaaaagt gatagtaatg gagataaaac agataatgat ttaaatgaag
    43501 ataagactac aaatgtgtca gaagcattaa ctggaaatag tgagactaat agtaataaca
    43561 cggataaaga tgataaaact ccattgtatg ataagatacc aaatgttata aatatagaag
    43621 gaaatgctaa agattcctct tatgtaggtc aatttttata taaattaaag ggattatact
    43681 atttcaagga tgttaaatta catagcataa aaagtaatgc agaagattct aactatacat
    43741 ttagtatgac attatacctg aaagaaggtg tattaactaa tgaataaaga tatatttaaa
    43801 aaagatataa aagatatatt taaaatggaa gttagtctaa aagggattct tataaaggtt
    43861 ataattttat tagctatttt atcatatggt tattttggac tttaccctca atatcagaaa
    43921 tatattgcta caaaaaattc tgtaaaacag gctactgaaa aattaaatat atataaagag
    43981 aaaactgcac ttatgccaaa atatgaaaaa caacttgaag aagttgaaag tgaattaaaa
    44041 gaaaaaagca aaaatctttt atatgatatg caagatggtc tttttttaat aggtttagat
    44101 aaaaaaatga aggcttataa tattgactta actagttatg acgttggaaa tgttaaagaa
    44161 tacgaacatt tttatggtat atcaacaaca ataagtgtta gaggtgatta tagaaatgtc
    44221 aaaaaattaa tggcttattt agaagaacaa aaaaatgtta ctcaaatact agattataca
    44281 atgacttcat atatagaacc agaaaaagaa gaggaagaaa caacaaacaa taaggattta
    44341 attaaaaatg tgaacataaa taaagataca aaaatatact gggtggaagg cgaaaataag
    44401 tggcatttat ataaggattg tccattgata aaagaaggag agattctaaa aaatgacaaa
    44461 cttttagata ggtctactaa ttatctatgt aatacttgtg tacttagagc tgcaacagaa
    44521 aaagaagcaa aaaaagaagt cgtagctcca aaggcagaag gtgtagtaga tgctacattt
    44581 aaatttataa tgtacactaa agagaatact acaatgatac ttgatacaga taatccatct
    44641 tcatggagac caggaaaata taatccattt aagtctagtg ctaaataaag ctaagtaagc
    44701 tttaaagata ggtgatgagt ttgtacaaaa ataatgaaaa aggtcttact ttattagaag
    44761 taataatagc tgtttttata ctgacaatag ttttaagtat ttcttataaa gtgttcaatg
    44821 gtataacatc agcagtaaaa aagcaacaga ttattacaga tgctcaagta aatattaatc
    44881 taattaataa gtatctaaat agagatttgg aaaactgtaa agagctaact aaaactggtt
    44941 caggtaataa ttatgaatac aatatagaga tgccagataa tgttgtaaaa tatgaagttt
    45001 ctatagaaac taaaaaaaat acagaggtat attctgtaac gagaatccaa aacaatacaa
    45061 ttgatacaga aaatgaagtt agagaagaga taatctataa tcaaccacta gttcaaaaca
    45121 ataaagaaat gaaggaaaca ccatttaaaa tagaaaagca aactggtaaa tctatttata
    45181 ctgtaagtat atactataat gaatcagtgc aagaaagtca taaaaatagc aatttaaata
    45241 acaaaactta tacatttgat gttatgtcaa gaatagggta ataagaattt attaaaaaat
    45301 aagtaggttg ataaaatatg agtaaaaaga atagtgaaag aggattttcc ttaattgaag
    45361 tgttagtagc tatggctatt atgggaattg tactatttgc attttttaat attatcaata
    45421 ctaataacaa agcaaacact aaaaatgaca cagatataac ttccttaaac tatgtccaaa
    45481 gtgaaataga aaatctaaga gaaaagataa aaagtggaga atttgatttt gatagtttag
    45541 ataaactaga agatggaact gtcgtatatg aaaaattaat agataaatca aaaaaagttg
    45601 tatatgataa agttcttagt gaaggtgacg tgagcttata tgatactcca tatgaaaaga
    45661 ttacaacaat aaaagatgaa gatggtaatt taatcgacaa aggaaatatt acaaataaga
    45721 taaagactat tgtagaagat aaaagtggtc agatatataa aatagctgta actgggaaaa
    45781 gcatgaatga ctattcaagt aaaaaagagg ttaaaattgt aactgaaata tttaaagata
    45841 aataatttta cttgggggtg agattttgag gaagtggaat aaatttaaaa gtgaaagagg
    45901 ggcagcttta gttttagttt tgattgtagt agcattgctt tctattgttg gattgatatt
    45961 ttcaaatcag atagctaata gaataaaatc tacaaagact actaatgaag gtatacaagc
    46021 aaagtattta gcagagacat gtgtggaaaa tagtattgat aaagcatatg aaaaattgta
    46081 tgatgagctt gagaaaatgg ataatgagtt taaaagtgaa aatcaagaaa aatccatatc
    46141 taggtcaaaa ttaagaaata tatcagatga agattttaat aatcaagatg agaaaaatat
    46201 tgaagcagaa agacttggat atatgaataa tataaatttc tatttaaata aagcttcttc
    46261 agacttggaa aaagctagta tggaattgaa aaagctatat gatttagata tgttggacta
    46321 tagagacatt gaatatgtag atgcaaatat tatttcacat agagattcta tcttagaaat
    46381 ttgcaagaat tatacttctg gagatattag taaaataaat gaatatattc tgaaagaaga
    46441 tatagactca actacattga ttgaggcaaa gttagtgaat aatgatattc ttttgaaaat
    46501 gtttttagaa gaaaataaaa ttgaaaatga acatttaaat tcagcctttt cacatactta
    46561 taaggcatta gataacattt ctttggcgat gcaaaatatg attgagtata gacatacgtt
    46621 tcatatagat gagccaaaag tagaagtatc taatgggatt ccagattctc aacaatatta
    46681 tgaactaata caaaatccta taataaatag tatggagtat atctggaata gtaaatggga
    46741 tacattagaa aacctattgg aaattttacc aaatcaaact caaggattta attctttaag
    46801 agttcatttg agaaataatg taagaaaatt tgagaagtta agcgataata tatctagtgg
    46861 taaaaaaaat acagcaaaaa attttttaaa gtataaagag ttattgtatg aaatatcaga
    46921 ccaatgtaat caactaaaaa gtatgtctta tgagaaaata cctgttaaat atgataatat
    46981 ggctttgata acaacttttg actatataca aaatgagctt ttagcagaga taaaatgcag
    47041 attaaaagaa ttaaaaccac aagaaattga taaaacagaa ggtattacta taaaaatacc
    47101 tttttataag gctgactacg acatgacaaa agaaggatgg cctaaattaa aagaaaatgg
    47161 ttcaggggcg gagttaagct taatggtaac tggtgataaa gatggaataa aagaagtaga
    47221 agttacagat ggtaaaaaaa atattattgg tttaggtgtt gaagaaaatt caaattcaaa
    47281 gtataaggtt gacgccattg taaactttaa tctaaatata gatacaaatg tagttggaaa
    47341 ttatgatata aaagataaaa ttttgattaa tcatgatata agttcataca aaaaagtaaa
    47401 ctaaatataa gaagcaactt agtataaata aataataaaa taattttatt atttactact
    47461 aaaaaaataa gtaaactaat agaagatatg gagagtaaat attatgtata tttatgattt
    47521 attagagcaa ggtataagac taaacgcatc agatatacac ataacagttg gaacaaatcc
    47581 tgtagctaga gtaaaaggtg gatttgtaaa actaagtgaa caaatattga catcagaagt
    47641 aacaatgcaa atggcaaaag acatagcggg ggaatctatt tttaaagtaa tagaagaaca
    47701 tggagaagct gatttttcag cttctctaaa aacaggagaa agatttaggg taaatgcata
    47761 taggcaaaaa ggaaattacg caatagcaat aaggactata acagcagaaa ttccaacatt
    47821 tgaaaaatta ggccttccag agagtataaa atcttttact gaaaagcata aaggattagt
    47881 tttagttaca ggtccaacag gaagtggtaa aagtacaaca cttgcaagta tgataaatat
    47941 aataaatgaa aaacaacaaa aacatataat tacacttgaa gacccgatag aatatgtaca
    48001 tcatcataaa caaagtttag taaatcaaag agaagtagga actgatacag aaagttttca
    48061 ttcagccctt agagctattt tgcgtcagga cccagatgta atactaatag gagagatgag
    48121 agaccctgaa actgtttcaa tagcattaac agcagcagaa acaggtcatt tagtattttc
    48181 aacactacat acagtagggg cagctaagac aattgataga atagtagaca tgtttcaacc
    48241 atcccaacag caacaaataa aaacacagtt atctacagtt tgtgaaggtg tggtatcaca
    48301 gcagttactt cctacagcag atgggaaagg cagaatagca gcaattgaat tgatgtttgc
    48361 tactccagca ataaaaaact tgataaggga aggcaaaaca tatcaaattc caaacatgat
    48421 acaaactgga gttaaagtag gtatgaaaac tatggaccaa gatttaatgg aactttataa
    48481 aaatggaaag attacaaaag atatggcact tagtaggtgt actgaccaag agtttatgac
    48541 aagaatgata ggtggagtaa actacaatgg atattataat cgttaatcta tatgtattta
    48601 tagtgggtat agtgtttgga agtttcttta acgtctgtat ttttaggatt ccagaaggta
    48661 tatctatttc tagacctcct tcccattgta tgagttgtaa tactagacta aagccaatag
    48721 acttaatacc agtgctaagc tatttgatga gtggcagaaa gtgtagatat tgttatgaga
    48781 aaatttctag tagatatgct atagtggaat taattacagg aattttattt ttaacagtat
    48841 ttatgttata tggaatatct acatcaacta tttattattt ggttcttgtg tctttactga
    48901 ttataattac atttatagat attgaccact ttataatacc agataaaata ttgatttttg
    48961 gagctatatt ttctatgatt tttaaccttt tatttaaaga aattccagtt aaggattcaa
    49021 ttctaggtgt ttttatttgt ggaggaagtg tctggattat agttcttttg attgaatttg
    49081 taataaaaaa agagtgtatg ggtggaggag atatcaaact atttgcaatg ctaggccttt
    49141 acatgggtgt taaaaatggt cttttgacag ccttactaag cgtttatgta ggagcagtct
    49201 atggaatctg tgtaataatt ataagtagaa taaaaggaaa ggaatataat tctgttatac
    49261 catatggacc ttttatatct attggagcat taataacaat cttatgtgga aagcaattac
    49321 ttgaacttta tttttctatg gttatataga tgatagtcaa gttaaataaa gttttaattt
    49381 aaaaaaatga attaaagtat ttaataagga caatattatt attaacccta ttactaaagt
    49441 gctacaaact atggagtata taataataat ttccttattt tttgcacatt tgataaataa
    49501 atctctatgt gttttttgtg atagagaaaa gaaaaattta tataatacgt tttttattta
    49561 cataataact gtagttttat ctgtattaat atatctaaaa tttgggatta gtgtagaatg
    49621 tgtacaatat atggttttaa ttccattttt aataattata tccattgtag attactacac
    49681 aatatatgta tatgatataa caatagttag tggtataata gttcaaggta taattttttt
    49741 ggcctttttc aatatagctc aatttgatta tataaatcat atatttggac tattgatagg
    49801 ctttattata ccttatatta ttgctaaact aacaaaagga ttaggctatg gagatgtagg
    49861 tctctatagc ttatgttgct ttgcattggg aaataattat gggttttatg tgatagtttt
    49921 atcatttatt ttagcgtttt tgttttttat tattttatac ataactaaaa tatttaaaga
    49981 tgtagatata agaaagcaaa ttccttttac accttttatt tccttgtcaa caattttaat
    50041 aatatttaca gagtataata tatttaattt ttattataat ttgttataca aatttatata
    50101 acaggaggta agaaatgtac gtagtagtag ggttaggtaa tccaggaaag aagtatgaga
    50161 agactagaca taacgttgga tttgatgtta tagatattct tgctaaagaa tacaatatta
    50221 gtgttactaa aataaagcat aaagctttga ttggtgaagg gagagtaggt actgaaaaag
    50281 tattattagt taaaccacaa acttatatga atttaagtgg agaaacatta atagatatat
    50341 ataaatatta taaggttgat ttaagtaata tagtagtagt atatgatgat atagatttag
    50401 aagttggaaa gatacgtata agaaaaaaag gaagtggagg aactcataat ggcatgaaat
    50461 ctataactaa gtgcctaggt tcaaatgact ttccaagagt gagagtaggt gtatctaaac
    50521 cagaagctgg tcaagactta gcagactttg tactttctag atttagaaaa gaagaatctg
    50581 ataacataaa tgaagcatta gaaaaagcgg cagatgctat tgactcaata ataagagaaa
    50641 atatagatat gtctatgaac aaatataatg gatagtgaga gggagattta aatgaatgat
    50701 gtttttttat atcctttgca aaattcaaaa gagtataaag atataataaa ctgtataaaa
    50761 aatacaaaag gctcattatt ggttaatgga cttttaccag tacaaaaacc tcatatatcg
    50821 tattctatat ttaatgattt atcacgacaa atgattttta taacgagtag tgatttagaa
    50881 gcaaagaaag tatacgaaga tttaagtttt tatatggaag ataaagttga atatttaggt
    50941 tttcaagata tttattttta tcacttggat gcaaaggaca gaaacgaaga agcaaagaaa
    51001 ttaaaagtat tgttaaaact agcaaataaa gaaaaaatca ttttagtaac ttctattgag
    51061 gcagttttaa gaaaatacat accaaaacaa gttttgctcg atagtgtatc tcattataag
    51121 gtgggagata gcttagactt agaaaagtta actgagaaat tagtttcatt gggatatgaa
    51181 agagtttcaa aaatagaggg gtttggtcaa tttagtataa gaggtggaat tattgatgta
    51241 ttttcacttg aatatacaaa tccaattcgt atggaattat ttgatgatga gatagattcc
    51301 atacgaactt ttgacgtata ttcacaaaaa tcaattgata aattgcaaca attctctata
    51361 actccatcta gagaatttat ttatcccgaa aaaactacag atgcattagt taaattaaaa
    51421 aaagaaacaa ctaaaaatac tgatgaaggt acatttcaaa atatagatta tatatcaagt
    51481 aagacttatt ttgaaggtgc agagaactat atagattata tttatccaga agaaaacaaa
    51541 agtatattta cttatttagc agatgatgca atagtattta ttaatgatat aacgagactt
    51601 aaagaaagat gcgaaaatta tataaatgag tttagagaaa attataaatt aaatcttgaa
    51661 agaggtctgg ctattaaaaa ccaaggtaaa ttgttatatc actatacaga tttagagtac
    51721 ttagtaaaag acaagtctgt ggtattaaac tcgctattgc caaagtctat aaataacttt
    51781 tctataaaat ctataataaa ttttgaaagt agagaagttc ctacatttaa tggaaaggta
    51841 gatttgcttg tagaagaatt aaatcggctg aaatataatg gatacaaaat aattttagca
    51901 acaaatacct tagaaagagc aaataaatta ggaaaagatt tattggacaa aggcttagaa
    51961 acgaccattt ctaaagatag agatattgaa attaaatctt cacaagttat aattgtacct
    52021 gcacacataa acagtggttt tcagtataaa tctataaagt ttgttgttat aacagataac
    52081 gaaatgatag gtgtttataa aagagcgtct aagactagca ataaaaaagt taaaaaagga
    52141 aagaaaatag aatccttttt agatttaagt gttggagact atgtagttca tgaaaatagt
    52201 ggtgtaggta gatacacagg gattgaacaa attacagtca atgctattaa aaaagattat
    52261 atgaaaatag tatatcaagg tggagataat ctatatgtgc caatagacca gatggacaaa
    52321 gtacaaaaat atattggtgc agaagtagaa aaagttaagt taaataagct tggaacaaat
    52381 gagtggacga aagcaaaagc aaaagttaaa aaagaaattg aagatatgac aaaagacttg
    52441 attgagcttt atgcaaaacg tgaaaaaata caagggtata agttttcaaa agatacacct
    52501 tggcaggcag agtttgaatc gttgtttcca tatcaagaaa cagaagacca attaaaggct
    52561 atagaagaaa ctaaaaaaga tatggaatca agtaaggtaa tggataggct agtatgtgga
    52621 gatgttggtt atggaaaaac agaggtagct attagaagta tttttaaagc ttgtatggac
    52681 caaaaacaag ttgctgtatt ggttccaaca acaatattgg cccaacaaca ttataataca
    52741 ttcaaagaga ggtttgaaaa ttatcctttg agagtagaag ttttaagtag gtttaaaaca
    52801 ccaaaacaac aaaaacagat tattgaagat gctaaaaaag gattagtaga tattttaatt
    52861 ggaacacata gaataatatc caaagatata aatttaccaa acttaggtct tgtagtcatt
    52921 gatgaagaac aaagatttgg agtaaaacat aaagaagctt taaagaaaat taaatctaca
    52981 gtagatgtac ttacactttc agcaactcct ataccaagaa cattacatat gtctttaagt
    53041 ggaataagag acatgagtgt tatagaagaa cctccacaag aaagacatcc tgtaataaca
    53101 tatgttacag agagtaagga aagcgtaatt caagatgaga tagagagaga attaagtaga
    53161 ggtggacagg tattttttgt atacaataga gttgaacaca ttgaagaaat ggcaagtatg
    53221 attcaaaaac tcgtaccaga tgctagagtg gctgttgcac atggaagaat gacttctaaa
    53281 tcattagaaa atataatcct aggattttta aataaagatt atgatgtact agtctgtact
    53341 acaataattg aaactggtat ggacatatca aatgcaaata caatgattat atatgatgca
    53401 gataaaatgg gattggctca actttaccaa ttgagaggta gggttggaag aagttcaaga
    53461 caaggatatg catatctttt gtatgaaaaa gataaaactt taagtgagat agcggagaag
    53521 agattaaaag ctatcagaga atttacagag tttggttctg gttttaaaat agcaatgaga
    53581 gacctcgaaa taagaggagc aggaaatatt ttaggttctc aacaacatgg tcatatggca
    53641 gttataggtt atgatttgta cgttaaaatg cttaatgatg caattaaaaa agtcaaaggt
    53701 gaacctattg tggaggaaat tgatgttgaa atagatttaa gtgtaaatgc atacattcca
    53761 gataattata tcaaagatga gttgattaaa attgaaatgt ataagaaaat agcttctatt
    53821 gaaaacaaag aagacatgtt ggatatacaa gaagaacttg aggatagatt ctctgatata
    53881 cctaaaccag tccagacact tcttacaata gcttatataa aatcgttatg taagatatta
    53941 aaaatagaaa agataagaca attaaaagat gaaatattat tagttccaat aacaaagtat
    54001 agaactaaac agaaaatagg ttataatata gttacagaat tagaagaatt attagaaaag
    54061 atgtgcaagg taaaataact tgtaaattta agaagggatg ttttaaattt gaaaaaagta
    54121 ataacattgg taatagctat gattttggta gtatctgtga ctgcttgcag ttctagtaaa
    54181 ggagaaactg tggcaacagt tgaaggtaca aagatatcat cagatgaatt taaaaaaact
    54241 atagctcttt ataaagattc tatggaacaa acttatggaa aagatatatg ggataaggaa
    54301 gttgaaaagg gtgtaaaata caaggacaag tttaaagatt taattcttga ccaacttata
    54361 actacagaag taatatattc tcaagctaaa aaagataact tacttcctaa aaaagaggat
    54421 gttgaaaaaa gttttaaaga attaaaagac gctatgggta aagatgaaaa atataaagaa
    54481 caacttaaga aattaggtat agatgatgag tttttaagag accaacaaga gaaagattta
    54541 gcaatgcaaa actatcaaag taactttgct aaaaagacta aaatatcaga tgaagaaatg
    54601 aaaaaatact atgatacaca taaagatgaa tttaaaaaag atgaggtaga agcttctcat
    54661 attttattga aaacagtaga tgataataac aagccacttt cagataagga aaaagctgaa
    54721 gctaagaaaa aggctgaaga ggctcttaaa gaagttaaat caggtgagga ttttgcaaaa
    54781 gtagcaaaaa aatattcaca agatacttca gcaagtgatg gtggtaaatt aggattcttt
    54841 tcaagggggc aaatggttgc tgaatttgaa gatgctgctt tttctatgaa aaagggtgaa
    54901 gtatctgatt tagttgaaac tcaatatgga taccacataa ttaaagtgac agacagaata
    54961 aatgagcaaa catcatttga agatgctaaa gaaactataa aagaccaact gttgaaaaat
    55021 aaatatcaag aacaaataga aaaattaact aaagaagcta aggtagaaaa agatgaaaaa
    55081 gtaataaata aaatcacaat ctaagactta aaaaaagagt aatatgctta aaaaagctat
    55141 atctggataa gatatagctt tttttgtata tttttcttaa caatggtaaa aataaactta
    55201 aagtaattta tggagggatt aaaaaatatg aaagcaacag gtatagttag aagaatagat
    55261 gatcttggaa gggtagttat cccaaaagag ataagaaaga cattaagaat tagagaagga
    55321 gaccctttag agatattcac agcaaaagat ggagaagtca tacttaaaaa atactctcca
    55381 ataggtgaat taaatgaatt ttcgcaagaa tatgctgaaa cattagctga agtactagga
    55441 tatggagtgt tgataacaga cttagattct ataatagctg tgtctaagct tcctaaaaaa
    55501 gattataaag aaaaaagtat aagtgatgag ttagaagcaa taataagtga aagaacagta
    55561 aaatattcaa aagataaaaa cattttacca ttattcaagg aagaccaaaa agaatatact
    55621 agtcaagtta tcatgcctat aattagtgca tctggtgact gtataggttc aattgtagtt
    55681 gtttcaaagg ataaagatac aattgcagaa agtgaagaaa aatctttaaa aattgctgct
    55741 aactttttag gaaaacaagt tcaataattt tatcataaag accttttaca tgaaggtctt
    55801 ttgaagtttt tgtgtaaaat attgaatata gcattaaaaa tatggtatat taaagtttgt
    55861 tatataaaca gtatggaggt tatgttatga acaataacaa aaataaagac tcctttctaa
    55921 aaggagccct tatacttggg ctagcaggtg taatcgtaaa aataatgggt ggatttttta
    55981 ggattcccct tggaaacatg attggttcag aaggaatggg atactatcag gctgcttatc
    56041 ctgtgtatac tctattttta acattagcta ctgctggatt tccaacagct gtggcaaaat
    56101 tggtttcgga aaaagttgca attggaaatt ttaaaggtgc aaataagata tttaaggtat
    56161 cacatacagt tttattcatt accggtataa tatcattttg tatattattt tttggagccg
    56221 actacatagt aacaaacgta atgaaaaatc caggtgcgtt atactctatg aaagccattg
    56281 ctccagcatt actatttgtt cctgcaatgt ctgcatatag aggatatttt caagggagac
    56341 aagatatgac taagattgct gtgtctcaag ttgcagagca attttttaga gttgtattag
    56401 gtcttacttt ggcctacttg cttatgaaga gtctaggtca gacttttggt gcagcaggtg
    56461 ctatatcagg ggctacaata ggttcaatag catctatgct ttatttggtg tttgcttata
    56521 tgctagggag aaaagagcgt agagcagaga tagatgcaag ccaaagattt aaagatgaaa
    56581 gagtgtctta tatttttaaa aagcttttaa ctgttgccat tcctataaca ataggagcat
    56641 cagtaatgcc acttgtaaat atgatagata atgttattgt aataagaaga cttatggaag
    56701 caggatttac atacaaagtg gcaaattcaa tgtttggtca attaacaggt atggcaatgg
    56761 ccacaataaa tttaccagca gtcataacaa ctgcaatgag tatgagctta gttccagcaa
    56821 tttctaaagc atatgcatta ggaaataagt caaaagctag aaaagatact aagagtgcag
    56881 ttaaggtaac tttacttata gtattgcctt gtgcgtttgg tatggcttct ttagcaatac
    56941 caataatggg tcttttattt ccacatgaac catcttctgt aggaactatt ttgtttactc
    57001 ttactccatg tgtattattt ttaggattaa tacagacatt gactggtata attcagggaa
    57061 tgggaaaacc aatagtacct gtaattgctt tatgtgttgg tatgctatgt aaaattgtaa
    57121 taagttatac tttaacaggt ataccagata ttaatgtact gggttcagca tttggtacag
    57181 ttacagcata ctttgtggca gccatgatta acttactata tgttaaaaaa cacatgaatg
    57241 taaacttttc taaaaaagaa tttataataa agccatttat aacagttatg acaatgttta
    57301 taatggttaa gttgagttat ggtgctttag ttggattctt aggaaacagc atatctacaa
    57361 taattgcaat atgtgttggt ggaattgtat atgtagtagt aatacttgga ataggtggaa
    57421 ttaaaaaaga agagattcta actatgccaa aaggtgataa attatataaa ttgttaaaaa
    57481 aagttaaact tataagataa tataggaata tatatgcaaa taaaggaaat actatacaaa
    57541 tagtaaatct ccttatttgt ataataatat aaaaataaag tgaaatttgc taataggagt
    57601 gatgttatgg gtaagataag tattgtaggt cttggaccag gagactattc tcttataagc
    57661 caaggtgtgt tagaatcttt aataaatagc cctaaagttt tttttagaac agagaaacat
    57721 ccaataattg ataaacttaa agaaactata caatacactt ctttagacta cttttatgaa
    57781 aaagaggaaa attttgataa tgtttatttg aaaatatctg aatttatcat agaagaatca
    57841 gcaaagggtg acctagtgta tgctgttcct ggtcatccaa gagttgcaga gaagactgta
    57901 agtataatag agagattagc caatgaaaag cacatagcag tagaaatatt gccttctatg
    57961 agctttgtag acgctatgtt taactatttg gctatagacc catctgaggg atttaaactg
    58021 gtagatgctt ttgaaataga aaattcatat atagacttgg atacaagcat gataataact
    58081 caggtatatg ataaatttat agcatctaat gtaaagttaa aacttatgga acattatgac
    58141 tataatcaag aagtatgtat agtaaatgga gcaggtgtca aagatttaga aagtaaaaag
    58201 tttataaagc tttgtgaatt agacagaaat gaaaactttt ttgactattt aactagctta
    58261 tatataccca aaagttctaa aaagatgtat aatacagtac atgacttaga aataatagtt
    58321 aatacactta gaagtccatc tggatgtgaa tgggataaaa aacaaacaca tcaatcatta
    58381 aaaaattctg ttatagagga agcatatgaa ctttgtaatg caattgataa taatgatata
    58441 gatgaaatgg tagaagagtt aggtgatgtt cttttacagg ttatattcca ttgtcagata
    58501 ggaaatgagg agggctactt tgatttaaaa gaagtagtaa atggcatatg taaaaaatta
    58561 attcatagac atcctcatat atttagtggt gcaaaattag atatgtctaa gtttgagaaa
    58621 acttgggaag aattaaagaa agaagagaaa ggcgaaagta ctataacaga tggccttaga
    58681 aggataccaa aacatttacc agctcttatg aaggctagta aagttcaaca taaagcagca
    58741 cttgtcggct ttgattggga caatgtagaa gatgtattta aaaaaattga agaagaatat
    58801 caggaattac ttgacgaaca cagggtcggg aatataaaat acataaagga agagctaggc
    58861 gatttattat tttcgatagt aaatctagct agatttttag acatagactc aggagaagcc
    58921 ttgaactgta ctacagaaaa attcataaat agatttgact ttatagaaaa aagtgcaatg
    58981 aacttgaata aaaaattaga agacatgaca cttgaagaga tggatgagtt ttggaatcag
    59041 gctaaaaaac aataaaattg tggaaggaaa tttgtcaaaa atctagaact tttatcacaa
    59101 agatagaagt ctatattata aacattttta ggaggtaaat atcgtgaata aagctgaatt
    59161 agtatcaaag atggcagaaa aaagtggatt aacaaagaag gaagcagaag ctgcgttaaa
    59221 cgcatttatg agttctgttc aagatgcact agtaaataat gaaaaagttc aattagttgg
    59281 atttggaaca tttgagacaa gagaaagagc tgctagacaa ggaagaaatc caagagatcc
    59341 agagcaagtt atagatatac cagcttctaa agcaccagtt ttcaaagctg gaaaaggatt
    59401 aaaggatata ataaatggat aattagaaat cttgtgaaaa agtgtggata aatttgccac
    59461 actttttcta tagaaccaag gaaggtgata attatgagat tagataaatt tttaaaagta
    59521 tctaggatta taaaaagaag aactgttgct aaagatgcat gtgataaagg gattgtcact
    59581 ataaatggaa aagttgctaa atcttcttca gaggtgaata ttggagatac attagaaata
    59641 caatttggag agaagaagat gaagtttaag ataaatgaag taaaagagca tgtattaaaa
    59701 aatgatgcaa aagaaatgta cgaaatttta gaataaaatt aatagtcctc tagtatatta
    59761 atagtagtat tattgaaata tttactgggg ggatattatg gaacagaaca taactttgaa
    59821 agatagatca aagctggtta tttcgggagt cgagcacata tactctttta atgacaaaag
    59881 agtagagctc aaaacttctg taggcgagat ggtaattgaa ggcgaaaacc tagatatgag
    59941 caagctaagt atagacgaaa atataattag catagatgga actataaatt ctatggtata
    60001 tgcaaagcct aaaaagccac aagagagttt cttgaaaaag gtatttagat agatgatacc
    60061 ttttactcaa gatgtaagca ttttttatgc tactatttat ggtggaatac tcataggggt
    60121 gttgtttgat ttttatagag ggctaagagg gaattttaaa ttcatcaatt attttgctat
    60181 aatctttgat gtattgtttt ggtttctagc tacggttata atattcgtaa caataaacct
    60241 tacagaattt tttgatttaa gatattatca ttttgtggct ttgtttattg gttttatttt
    60301 atactataac accataagta aaattgtttt atcaattatc aataaaataa ttcgttttgt
    60361 gagaaattct tttaaaaaag ttacacatta catagtcagt tttttaaata atttgtatta
    60421 cgttataatc tattcactac acctactatt tgatattata ttttatatac caaacatatt
    60481 tatagcaaca agaaaaagta taaaaagaaa atcaaataaa aaattaaaga acaagaaaaa
    60541 atcaaaacct aagaagaaaa aaaataaaac taaaaaaagg gtgtaggaaa aatgaactta
    60601 agaaaaaaat tttcaggaca gattattgtt atcagtttat ttttaggaat atcaattttt
    60661 agtatgatga caggatttgt atttgagtat acaaaagcta aggaatataa aaaagaaata
    60721 gcaagtctaa ataaacaatt aaaaaagact gaaatacaaa taaacagctt aaagaaagat
    60781 gaaaaatcat atgaaggaga cttagaggat atagcaagaa aaagattgaa tatggtaaaa
    60841 ccaaatgaaa cagtttatgt agacataaat cgttgattaa aacttgctaa taagtttaca
    60901 gatgtattga ttttaaaatt taagatttaa taaaaaagtc attcagttaa ggatggcttt
    60961 ttgtttttat tgaggaggga atttcataat gaaagtagga gctatagata ttgggacaaa
    61021 ctcaatgaga ttactgatag cagattatag agatgggaat tttataaaca gaaaaaaatt
    61081 tgttaataca actagaatcg gttatggtgt ggatagtaaa ggatatataa aagaagatgc
    61141 tatcaaaaga aatataaaag cattaaaaga attttctgat aagtgtaatg atgaaaagtg
    61201 tgaaaaaatt tattgtatgg ggacatctgc tcttagagac agtaaaaatg gagattactt
    61261 tgttaaatta gctaaagaag aaactggaat agatgttgat atagttacgg gaatagaaga
    61321 atcaaatttg ggctttaagg gtgttttaga aggaatacaa ggtgatgata ctattattgt
    61381 tatagatatt ggaggaggtt caacagaatt tattgtaggt gattcagaag gaattaaatt
    61441 taataaaagt gagaatgtag gtgctcttag gatgacagag aggtttttga agcacgatat
    61501 tattgaagat gaagagtttg aagctatgag ttcatttata tatgatgaga ttgacaaaac
    61561 gttaaatcat ataaaagaca gaggattaaa aactcttata ggtataggag gtacgattac
    61621 atcattatca gcaatgaatc aagaattaga gatttactct atggaaaaaa tacataatag
    61681 taaaatacac aaaaaagaaa tagaagaaat tctacaaaaa ttaataaaga tgacattaag
    61741 tgataaaaag aacttaaaag ggctacaaga aaaacgtgcg gatattataa ctgctggtgt
    61801 aaaaatattg aatataataa tggaaaaatt agaaatagaa gaaataaccg taagtgaata
    61861 cgataatttg gaaggcttaa tatgtcaaaa gtcaaaaaaa atgtattaat attttttgat
    61921 ttttaaaacc gattatgaca aaaaaaatta ctcctctttg ttacaattct taatataaac
    61981 aaaagaggag gtttttttat gcaaagaagt gtagcagttg tgaagaataa gactgtaaaa
    62041 ccaaatgtca taaaattaat aagcggatat attgatttta ctacggcggc attcgctgtg
    62101 atgggatttt tacttagtag gtctattata gtagactctg tagctccttt aggaattgcc
    62161 ttttttatat gtgcatcaaa gattgataag tacaagatac ctgtattcat atcaacatta
    62221 ttaggaatat tgttatcatc taatgatttt ggaaatgtag caaaatatgc tgtgtgcctt
    62281 acaatattta tggctatgaa taaaaaatta aaattatcag attcaattgc taaggttgca
    62341 tttataggtg caataatact acttccaatc tccatgggac aagctttctt ttcaaatagg
    62401 tatgtatatg gtcttcttat agcaggagta gaagcaataa taatgtttat atctatatat
    62461 gtgttttctt atgggatagg tttaatggcc aatgtcaaca ataggatgtc tgtacggaca
    62521 gaagaagcta tatcaataag tttactgcta acctttagca taatgggaat aggagatata
    62581 tctctgtttg gaatatcaat aaggaccatt ttatcgaccg tattgatatt gctgacctca
    62641 atagtaggtg gagctaccat gggagcatct agtggtgtaa ttattggtgt agcatccatt
    62701 ttaaataata tgacatcagc tgtttatatg gggatatatt ctttttcagg attaataagt
    62761 ggagcattta ataaaataaa taaatatttc tgtatactag gatatattct aagttggaca
    62821 ataatatacc tatatacttc tggtattact tctaatatga tgcaattaag agatatacta
    62881 ttaggttgtt tgatagtact agtattgcca gagagattat ttaataagat tgaaaaatta
    62941 ataaaaagta atgtggcttc aaatgaaatc gtatacgatt atattatgag aagcaaaaac
    63001 ctaactaata gcagattgaa cagtatttat aaaacttatg atgaccttgc agatacattt
    63061 gataagataa gagaaaaaga caaagtgcta gaccaaagag atatagctaa tgtaatagat
    63121 atgattcata atgatgaatg taaaagttgt agtatgagaa gaatgtgctg ggagtcaaga
    63181 tttaatcata catacactat gatatataac attcttgaaa aaattgagga gaaaggggag
    63241 ttaagtctaa acgatatacc taaaaatttt agaaaggaat gtatgaaacc agagtcgata
    63301 gtgaaaatat caaatcacta ttacaaaatg tttgtattag attatgattg gagtgtgaag
    63361 ttttcagaaa gtagaaagtt aatagcaaat cagataagaa gtatttcaaa atccattaaa
    63421 tccttatctc aagatttaga aggtgacata atgctagata tagaaaagga aaaaaatata
    63481 tatgaacagt tagaaagata tgatataaca gttgataaag taagttactt aacaaaaagt
    63541 aacaatgagt ttgaaatatc aatagaaaag aaaacttgtc atgatggatg tatgtgtgaa
    63601 gataaaattg taaatataat ttcagaccta gtaggtgaaa atatgtctgt tagaaaaata
    63661 ggatgtcact gtttaggagg taaatgcaag gcaacattcg ttaaatctca aaaatataag
    63721 gctgttacag aagtttcagc aatgtcaagg gatgggcata tcctttgtgg agacaattac
    63781 acatacatgg agataaatga tggtaaatac atgatggcta taagtgatgg tatgggaaag
    63841 ggtaaaaaag catatgaaga atcttctgcc actattgata tactggaaaa aatgatagat
    63901 gcaaaaatta aagatgagat agtcattgat actataaata atatgttgtt gttaaaatct
    63961 tcagaagaga tgttttcaac tctggatttg ggaattttag atttaaaacg aggttgttta
    64021 gaaactataa agatgggagc gtgttctact tatataaaaa gagaggatgg agaagttgac
    64081 ttaatatcat catcatcatt acctgtgggt atattatcag atgtaaaaat tgatagaaaa
    64141 aatgtaaaag ttaaggaagg agactatgtt ataatggttt ctgatggaat tgtagatgca
    64201 ggaagaaata acaatttagg agacaattgg ttaatatact tcttaaagaa tatagagact
    64261 acaaatccaa aggaaatttc aaatttaata ttggatagag cattagagtt acaagcgtta
    64321 caaatcgaag atgacatgac tgttttagtt acgaaaatat gtacaaaata aggtttacaa
    64381 gattatgtgt ataagatgtg gataacttgt gtataatatg ttaatagact tttgaaaaaa
    64441 gtatagttat taacaaaaat aacatataaa tcaacaaaat ttgttgataa gtatggcaga
    64501 aatgttaata aaatgtaaaa aaagaaaaaa caaaagctat tatcaaaaga gattcatgtt
    64561 gaaataacta aatttctaag taaagaaaac ctgtgaatat gttgattatt atgtggataa
    64621 ctattaattc gatatttatt tatatagtat gaataattga ataatatatc taaaaaaagc
    64681 catcctatta ttaagtaaat ttattgaaag gatggctttt tatgaataga aaattgtgca
    64741 aagcaataat acctttattt atttctattt tttgcatgtt aataataagt ataaaagctg
    64801 accaaggata tcaaaagaat aatgacagag caattgctgt catgaataat aatgatactg
    64861 aagatgatgc tgaaaaaaaa caagaagtag actatgaaaa aacatgttgg aatttgcaat
    64921 ctttgttcaa aagtgatgac caatggaaaa aagaacttaa gagttttgat aaagatacta
    64981 aagagttgaa aaattacata ggtaaagtaa caaaatcgcc aacacatttg tcttttgcct
    65041 taaacattaa ggaaaaactt gatataagac taaataaact ttctgcctat gtaaaattaa
    65101 aacaagatac taataaaaat tcatataagt acttagatat gagtgatagt atgagtaaat
    65161 catatgggaa ttatatgggg atttgttcag atttagaatt agaaatacta aaattatcaa
    65221 acaaagatta caagaaaatt atttcaaata aaaatataaa taggaaatat ggtgcatatt
    65281 taagagatat aagaaggaat aaagtacatt atttggatga taaatcagaa gatattctaa
    65341 gcaaagtatc ttcaataagt agtcttccaa gccaagtata tgaattattt agaaatatgg
    65401 atagaaaaac taatttgaca ccagcgcaat atgcaagtga attagagtca gtagatagag
    65461 aaaatagaaa aaaagcttat caagatgaac ttataactta caatgataat ataaatacaa
    65521 ttgcaggttt aattactggt caagttaaaa agaatatttt ttattctcag gaaagaggtt
    65581 atgagtcttc tttagaaatg tacttggaat ctgataatgt agatgaaaaa gtatacaata
    65641 atttgataag gactgtaaat agtaatatgg acagtctaca caaatatata gatttaagaa
    65701 aaaaagtatt aaaattagat aaggttcatt catatgatat gtttgtgcct atggttaagg
    65761 ctgttgataa taatattaat tatgaaaagg cacaaagtat agtatattca gcgttgtcac
    65821 ctttaggaaa agaatacaat gatgtagtat ataaagcttt caatgaaaaa tggatagatg
    65881 tttattcaca tgaaaataaa gtaagtggtg gatactgctt atctgtttat gaaaatcatc
    65941 catatatact tttaaattat aataattcat taggttcagt ttcgacttta tcacatgagc
    66001 ttggacatgc tgtatatgaa tacttaagtt ctaagaatca gaattatttt aattcaaatc
    66061 cgtctatatt tactcacgaa gttgcatcta caacgaatga agcattactt tacgagatgc
    66121 ttataaaaga agctaaaggc aatgatgaaa aagcttatta tataacacag tatttagact
    66181 tgataaaaga tactctttat acacaaacta tgtatgctga atttgaaaaa actatacacg
    66241 agttagtaga aaaaggaaaa agtgtaaacg cattagtatt aaatgatgtt tggggacaac
    66301 ttcttaaaaa atattatgga caaagctatg aattagacca attatctaag gtcggatggg
    66361 ctagaatccc acatttttat aatagtttct atgtctataa atatgctaca ggttgtagtg
    66421 caggagtaag ttttgcccaa gacatcctaa agaatggttc agataactat atgagctttc
    66481 ttaaaaaggg aagttcagat tatccaataa acttgttaaa atcaagtggc ataaatttaa
    66541 ctaatacaaa acccatagaa aatacaatca aaaaatttga tagtttagta gcagaacttg
    66601 aaaagctatt ataaacatta ttgtaatcca catggtaaat atatcgaatt aatgataaaa
    66661 tatactaaaa gtttaaagtc ctatcaagat aggacttttt atttattaat tagtgggaga
    66721 tgattctatg caagttaaag ttaaaaaggc tgtaatacca gcagcaggtt taggaactag
    66781 atttttgcca gctacaaagg cacaaccaaa agagatgtta ccaatagttg ataagccaac
    66841 tctacaatac ataatagaag aagctgtggc ctcaggaata gaggaaatac taataataac
    66901 aggtagaaat aaaaaatcta ttgaagacca ctttgataaa tctgtagaat tggaacttga
    66961 tttagagaaa aaaggtaaga aagaattgtt ggaggtagta caaaacatat ctaacatgat
    67021 taacatacat tatataagac aaaaagagcc aaaaggattg ggagatgcaa tttattgtgc
    67081 gaggcatttt ataggtgatg agccgtttgc agtaatgtta ggggatgata ttgtagataa
    67141 tgatgtacct tgtctaaaac aactaacaga tgcatatgag gaatatagaa ctacaatatt
    67201 gggtgttcaa aaggtaaatc aagaagatac taataaatat gggataatag aagcaaaaaa
    67261 tatagaaggt agagtttaca aggtaaaaga catggtagaa aagccagagt caggaaaagc
    67321 tccttcaaat attgctattt taggtagata tataataact cctgaaatct ttgatattct
    67381 taaggattta ccaccaggta aaggtggaga ggtacaactt acagatgctt taaaaatatt
    67441 atctaagaaa gaagctatgt atgcatacaa ttttgaaggc aagagatatg atgttgggga
    67501 caagctagga tttttagaag ctactgttga ctttgctcta aagaaagaag accttaagga
    67561 agattttata aaatatttga aacatgtatg tagtgaattt gataaaaata acaatgtgtt
    67621 atacaataat acagatgaaa aaatagattt aattgaagtc aatgaagcta gagaacttga
    67681 aattcaaaaa taattaatga aaggtgatgt atggtggcaa aatcaagtca gaaaagaatt
    67741 aatagaataa tagctgttgg gattgtggga atcctcgtaa taacgagtat aacagctgtt
    67801 ttgagtatga ttgcaactat gtaataagat aaatgacagc agttgatata cataaatata
    67861 aaaaccatat ataacttgat gtagtcaaaa acttacaata ttttagttat atatggtttt
    67921 tagaatttat aaatgtaaaa aaaaattttt ataatagttg catattttaa aggaaaatat
    67981 tattataaga tataaatata gtgataaatt tataaaaacg tgctaagatt ggacttgaaa
    68041 tacagtaata tagtaggtaa taatgtttta aattttattt attgaccata atatataaat
    68101 aatgatatta ttagacttaa ctgtttaaat aaaacttttc tttttcatgc cttcaatttc
    68161 caaaataagt atgtatataa atgaatatat agatgaggag gatttttttg attttagatg
    68221 ataaagtatt agaaggatta ggtagtaaga ccttagttga gctacgagag atagctaaag
    68281 aattaaaaat aaaatcaatt actacatata agaaaaatga gttaattgag attataaatt
    68341 ccaaaagtaa aaatgaggaa aaagtaaacg aagagactaa agaaaaaaat gaagatatta
    68401 aagaagacat aaagaataga aaagaaaata tagagcgtga aagtatagaa aaagaagttg
    68461 aatataagag ccctaccaaa tcatacaaca agaaagaaat agatacccaa aatatttccc
    68521 aaaaccaaga aaatagaaat caaagaaatg actataataa gacaacgagt gtaaatgata
    68581 gtaataagtc atcaaatata tctaatagaa tggtcagaaa taataataaa aactattata
    68641 tgcctaaaca agttgatgaa tctaagatag tagatgaatt taacacatca aaagaggatg
    68701 aagtagtagg tgtacttgaa attctgccag atggatttgg ttttttaaga ggttcaaatt
    68761 atttgtctac tgaaggagat gtttatgttt caccatctca aataagaaga tttaatatga
    68821 aaacaggaga caagattaag ggaataacta gacatccaaa aagtggagag aaatttagag
    68881 cacttttata tgttcaaaaa ataaatgatg aaaaccctga cacagctata caaagaaatg
    68941 cgtttgagac attaacacca atatttccag aagaacgact tacactggaa actaatagaa
    69001 atgaaatagc tacaagaatt atagatttaa tttcacctat aggaaaggga caaagaggat
    69061 tgatagtagc acctccaaag gctgggaaaa cagttctttt aaaaagcgtt gcaaatagca
    69121 tagctaaaaa tcatccaaat gttgaactta tagtactcct tatagatgaa agaccagagg
    69181 aagttactga tatgaaagaa tctattgaag gagacgttat atattcaact tttgaccaag
    69241 tatcaagtca tcatgtcaag gttgcagaga tggtgttaaa tagagctcaa agattagttg
    69301 aacatggtaa agatgtagta attttactag atagtataac tagattggca agggcatata
    69361 accttacaat atctcctaca ggtagaactt tatctggagg tattgaccct ggtgcacttc
    69421 atggacctaa aaagttcttt ggtgcagcaa ggaatattag acagggtggt tctttgacaa
    69481 tattaggaac agcattggtt gaaactggtt ctaggatgga tgatgtaata tttgaggaat
    69541 ttaaaggtac aggaaatatg gagctacatt tagatagaaa attagcagaa aaaagaatat
    69601 tccctgcaat agatatttac aagtctggta caagaagaga tgatttattg cttgatgatg
    69661 aagaaaagac tgctctttgg agattaagaa gagaaatgag taataattca gtaatggaaa
    69721 ttacagataa agtcattgaa cttataaaga gaactaagga caataaagag tttgtaaaat
    69781 caataaagaa tttgtaaaat caataataca aatgcaaata tggattatat aaactaatag
    69841 aaaaatcact tgaatggcat aataagttgt gctataataa tgtagttgat aatgtaaaaa
    69901 gaaagttaat ataattaatt tttttaaata aagagaagag gtgactataa tgcaaaagga
    69961 aatacaacca aaatacaatc cagttgaagt gcgttgtgct tgtgggaaca catttgttgc
    70021 tggttcaact aaggacgaaa taaaagttga aatatgttca gagtgccatc cattctatac
    70081 aggaaaacaa aagaacatag aaaaaggtgg aagaatcgac aaattcaaga aaagattcaa
    70141 aatggactaa tttttataaa agtggagttg gaggaagcca actcctattt tattacaaaa
    70201 aaacattcat attctacttt aaattgtctt tttgtattat aataataaat atgtaaaatt
    70261 gagtattcta aatacaataa aatgaggtga gtggatggca aaacaagctg taggtgggca
    70321 agctgttatt gaaggtgtaa tgatgcagtc aaaagacaaa agagcggtag ctgttagaaa
    70381 aagtgacgga gaaatagcac taaaagaaga tagaataaaa agttgggtaa gagataagaa
    70441 tatagataag ataccttttg ttagaggttc ttttgttatg atagatacaa tgattcaagg
    70501 gataaaaagt ttaaactttt cttcagagtt ttttatggaa gaagctgaag aggataaatt
    70561 tgatttattt ataaagaaaa tttttaaaga taaggcaaat gatataatta taatattttc
    70621 attggtcata gcaatgttac tttctgctgg attattcatt tttataccta cattggtagg
    70681 aggagctttt tctaaagtaa tgcctaatga ttttatgctt aatttaatag aaggaatcat
    70741 aaggatagct atattatttg catatatagt attgatttca cgaagtaagg atatagaaag
    70801 agtctttcaa tatcatggag cagaacataa atctatatat tgctatgaaa atgatttaga
    70861 acttacagta gagaatgcta gaaaatttaa aagattacat cctagatgtg gtacaaattt
    70921 cctatttata gttatggctg tatcaataat attgtttgca ttttttggtt ggccaaatcc
    70981 aattcttaga atatttatga gaattatatg tgtgcctata gtagcaggtt tatcttatga
    71041 ggtaattaga gttcttggga aatatgataa tggatttact aaaattattg catatccagg
    71101 aatgatgttg caatatttta caacaaaaga gcctgatgat gagcaattag aggttgcttt
    71161 ggaagcattg aaagcagtag tagattagag aaaaggatat ttgttatgac gataaaagat
    71221 ataattatta aatattcaga taaactaaaa gatataagtg atactccaag attagataca
    71281 gagttattgt tacaaaaaac attgggtgtt gatagattat atattcactt aaatctgaat
    71341 aaagagctaa ctgaagaaca aaaaacaaaa tttatgggtt ttgcagaaga aagattaaat
    71401 ggaagaccaa tagcatatat agttgaaaat agggagttta tgggattaga tttttttgtt
    71461 aaggaaggcg tattgattcc aagaccagat acagaaactt tagtagaaga aataattgaa
    71521 atatgtagag aaaaaaaaga tgtaagtata ttagatatag gaactggttc tggagctatt
    71581 acaataagtt tagcaaagta cattgaaaat tctaaaatca tgtcttttga tatatcagag
    71641 acagcattag aaatagctaa aaaaaatgct attataaatg aagttggtga aaaaataaag
    71701 tatattaatt ctgatttatt tacagcaata agtgatagta acataaaatt cgatatcata
    71761 gtttcaaatc caccatatat taaaaaacaa gatatagaaa ctctgcacaa acaagttaaa
    71821 gattatgaac catataatgc tcttgaaggt ggagaagatg gattagactt ttatagaaga
    71881 ataacagaac aaggtaagaa atatttaaat aagtgtggaa tattagcata tgaagttggt
    71941 cataatcaag ctgaagatgt aataaatatt atgaaaagta atggatataa aaaaatatac
    72001 acaaagaagg atattcaagg cattgataga gttgttatag gttacaatat atgatataat
    72061 caacaattga tatgttacat ttaatcgagg tgaaagtatg ttaaaaaaat tagaggtatt
    72121 ggaagataca tataaagact taagtgaaaa aataggtgat ccggatgtaa taaatgatca
    72181 aaaagtttgg cagaaatata taaaagaaca tgctgacttg gaacctattg ttatgaaata
    72241 cagagagtat aaaagcgttt tagatagtat aaaagaatca aaagaaattt tacaagaaga
    72301 gtcagatgaa gaattaagag aattagctaa aatggagtta gctgagatgg aagaaaaagt
    72361 agcacctctg gaggaagaaa taaaaatact attattgcct aaagacccta atgatgataa
    72421 gaacgttata gttgaaatta gaggtggagc tggaggagat gaagcagctc tatttgcggg
    72481 tgatttattt agaatgtact caaggtatgc tgaaagaaga agatggaaaa tagaattatt
    72541 gagtgcaagt gatactggag ttggtggata taaagaagta tcttttatga taaaaggtaa
    72601 aggtgcttat tcaagattaa agtatgaatc tggagttcat agagttcaaa gaataccatc
    72661 aactgaatct ggtggaagaa tacacacatc aacttctact gttgcagtat taccagaagt
    72721 tgaagatgta gaggttgaaa taaatccaaa tgatttaaga atagatgttt tccgttcttc
    72781 gggtaatgga ggacaaagtg tcaatactac tgactctgct gttagggtaa cacatatacc
    72841 tacaggtgaa gtagtatctt gtcaagatgg aaaatcacaa ttaaaaaata aagagcaagc
    72901 tttaaaaata ttaaaagcta gactttacga taaagcattg gcagaacaac acaaagatat
    72961 agcagcagaa agaaaaagcc aagtcggaac aggagacagg tcagaaagaa taagaacata
    73021 taatttccct caaggtagaa taagtgacca tagaataaat ttaactttat ataagttaga
    73081 tgcattttta gatggagata tagatgaaat gatagatgct ttaattactg ttgaccaaac
    73141 agaaaaaatg actgcaatat aagtaatgtt ttaaagaagc tgtttcttcc tagaaattat
    73201 tttctatttt gaaacagcta ttttgcattt taggtcatgt tatcatattc tattgtatat
    73261 aattgttata tacaagtata ggagggttat atgatactaa aagtaacaat aattgggctt
    73321 ttggcaggtg ttatagggac aggattagga ggagtaatat cagccatttt taaaagagag
    73381 gtcgataaat atctaagttt ttttatggga ttatctggtg gaataatgtt ggctgtagtg
    73441 gtgtttgatt taatgaaaga atccatggac aaaatgggga taatcaatac agttatattt
    73501 acgtttgttg gagctctcat aactatgtac ataaaaacaa aattagatgt gtctggaaat
    73561 atggcatctg gatatcttat attcataagt atattactac ataatttacc agaaggacta
    73621 gccattggtt cttcttttat gtcaactgaa agtttaggta ttacacttgc tatagttata
    73681 ggtctacata atataccaga agggttagct atggcattag gcttagtttg caataaaatg
    73741 aaactaagta aagttatttt gtttactgta atagctggat tacctatggg tctaggaagt
    73801 tttttaggtg tttattttgg aggagtattt acttctctta taggagtatt tcttgctaca
    73861 gcaggaggta ctatgatgta tgttgtattg gaagaaattt ttccacattc aaaaagtgtg
    73921 tattgtataa ttggtttctt gttaggtaca atgattgtaa attatatata aaattaaaat
    73981 gaaagcaggc atacaaaaga tgaaaactat tataagtaat attgatataa ataatattga
    74041 ttatgaggaa ataaaaatac aagcaaaact tcttagagaa ggtaaaacag ttatttttcc
    74101 aactgagaca gtatatgggt tgggtgcaaa tgcattagat gaaaatgcag taaagaagat
    74161 ttatgaagct aaaggaagac ctagtgacaa tccattgata gtccatatat atgaaaaaga
    74221 agaagtatat gacttagcca aagatataag tgataaagct aaacttgtta tagaaaagct
    74281 ttggccagga cctataacaa ttatattaaa taaaaaagat ataattccat acaaaactag
    74341 tggagggctt gatacagttg caataaggat gccttctaat gttattgcca gggcaataat
    74401 aaaagaagct ggtataccta ttgcagctcc ttctgcaaat atctcaggtc gcccatctcc
    74461 aacaaaagca aaacatgtat atgaagagat gaatggaaga gtggatggaa tagttttagg
    74521 aggagacagt aattttggat tggagtcaac tgtattggat ttaacagaag agactcctat
    74581 gatactgaga ccaggaagta taactaaaga agttttagag gaacttcttg gagaagtaaa
    74641 gcttgaccca tcattaagta aaaaagaaga taatcaaaaa gctaaagctc ctggaatgaa
    74701 atataaacat tattctccaa atgcagatgt atatataata tctggaaaaa gagaaaatgt
    74761 agcaacaaag attaatgata tgataaaatc taatagagac aaaggtctaa agacaggtgt
    74821 aatgtgtatc agtagaaacc gagagtttta tgatggagaa gtaatcgatt taggaaactc
    74881 tcttgaagaa gttgcaagta atctgtttga tgctcttata gaaatggaca aaaagggcgt
    74941 tgatgtcata tattcagaag agttccctaa gactggagtt ggacaagcaa ttatgaatag
    75001 actactgaag tcagcaggat acaaagtgat aaaggcttaa gagggagttg taaaatatga
    75061 atatacttat tgtgtgtaca ggtaatactt gtagaagccc tatggctgag gctatactaa
    75121 ggaaagcaat taaagaatct ggaagaagta ttgaagaata ttcgatatca tcagcaggta
    75181 tatctacagc aaatggaatg ggtgcttctg aaaattctat agaagtacta aaagaaatcg
    75241 gtatagactt atcaaatcat agaagtaaag taattactaa aaaattaatt gatgaatcag
    75301 atataatact aacaatgact aaatctcata aagaaatttt agtacaagca gttccaaaat
    75361 gcaaagaaaa agtgtatact tttaaagggt ttgcaaataa aaatgaggaa gatatatctg
    75421 acccatttgg tggaaatttg gatatttata gaagcactat gagagaaata atgtattctg
    75481 tcaacgaaat agtaaaaaaa atatgaaacg gaggataaac aaatgaagat aggattaggc
    75541 tgtgaccatg gaggatataa tttaaaaaaa gaaataataa gctatttaga aagtaaaggt
    75601 atagaatgtg tagattatgg aactaataat gctacagatt ctgttgatta tcctgtttat
    75661 ggagaaatag ttgcaaattc agttataaat aaagaagtag attatggaat attatgttgt
    75721 ggtacaggga taggaatatc tttagctgct aataaggttc ctgggataag atgtgcagtt
    75781 gtatcagatg tattttctgc aaaaatgtca aaagcacata atgatgcaaa tatgttatca
    75841 ttaggcgaaa gagttttagg aaaaggtcta gcacttgaga tagttgaagc atggattaat
    75901 acggattttg aaggtgatag acatgctaga agagtaaaca tgattaaatc aatagaagaa
    75961 aaacataata aataataaaa attacagttt tatcaggagg aattatcaaa atgagcaaag
    76021 tggtagagac aaaccatcca ttaatacaac ataaattaac tttaatgaga gataaaaata
    76081 ctggttctaa agactttaga gagcttttaa ctgaaattgc aatgttaatg gggtatgaaa
    76141 taacaaaaga tataccatta aaagatgtag aaatagaaac tcctattcaa aaaacatcat
    76201 ctaaagtagt tgcaggtaaa aaattagcaa taattccaat tttaagagca ggtcttggaa
    76261 tggtagatgg attagtaagc ttaatgccag cagcaaaagt tggacatgta ggactatata
    76321 gagacccaga aacattaaaa ccagttgaat attattgcaa gcttcctcaa gacataggag
    76381 aaagagatat aatagtagtt gaccctatgc ttgcaacagg gggttctgct gtagcagcta
    76441 tagatttact taagtctaaa ggagctaaaa gtataaaatt agctaattta gtagctgcac
    76501 cagaaggtat agcagaagtg caaaaatatc atgatgatgt tgatatatat gttgcatcag
    76561 ttgacgaaag attaaatgaa catggatata taattcctgg tcttggtgat gctggagata
    76621 gattgtttgg aactaaataa aaataaataa ctataaatat ttattaggga ctttcttata
    76681 tagtgtatat aattttatgt aagaaggttc ttttttatat attttttctc aaaataggaa
    76741 ttatgaattg ataaatttct cgaattgaga ataaaaagtt tttaccaatt aaaaaagatt
    76801 gcttatttta caaggtttta tagaatagta gtctatttta atgtggcatg ttagttgctt
    76861 acgattaaga ttataggtat taagtgtatt agttaaattg agaattgaat attaataata
    76921 ttattgggag tggtaataca tgaagaattg tatagcagta agaggggctg gagatttagc
    76981 aacagcagtt attcataaat tacacaacag tggttttaaa gtagttgcgt tagaaataga
    77041 agagccatta gctataagaa ggcaaatttc tttttgtgag gctgtttatg aaaaaaaagt
    77101 aactgttgaa ggtgtagaat gtatattttg caatactgaa aaagaaatag aagaagtttt
    77161 atctcaacat aaagtagcct tgattataga cccaaaagga gaaaaattat ctaagttaaa
    77221 tccaatcgtt ttggttgatg gaatactcgc taagaaaaac ttaggtacaa ctaaagatat
    77281 ggcaccactt acaatagctt taggtcctgg attttgtgca ggagaagatg tagatgttgt
    77341 tatagagaca atgagaggtc ataatctagg aagaataata actaatggta gagcagaaaa
    77401 aaatacagga gtaccaggag caataaaagg tttttctaaa gagagagtta tgcattcaca
    77461 gtatgatggt ataatggaaa atgtatgtaa gataggtgat atagttgaaa agggagacct
    77521 actttgttat attaataatt atgaaaaaaa acatgaagtt agagcaacaa tatctggagt
    77581 ccttagaggt ttattaaagg atggagcaaa ggtgaacaaa aatttaaaaa tactagacat
    77641 agaccctaga aaagaagaag ttaagaatgc ttttacaata tcagataaag ctagatgtat
    77701 agctggaggg gttttagaag ccatactaaa caatgggatt tatccccaaa attaatttat
    77761 attagataaa gctattaata ttttattaag atgatataat aaatttaata gattaaagag
    77821 agcatatgac gggtatttaa aatttaaatg gtaaaaatgc tcttttttta ttggacattc
    77881 aattaacatg tgtatataat ataaaaataa gtatttattt aggttgaaat ctaataaata
    77941 tataatacca gtacataagc aaagaggaga aaagtaaatt atgagaccat catgggatga
    78001 atacttcatg gaaattgcag aagtagtgaa aaagcgttca acttgtatta gaagacaagt
    78061 aggagctgta attgtaagag acaaacaaat attaacaact ggatataatg gttctccgag
    78121 aaatcttgaa cactgtgaaa atattggatg taagaggcag gaattaaata ttccctcagg
    78181 agaaaggcat gaattatgca gagcattaca tgctgaacaa aatgcgataa tacaagctgc
    78241 tcataatggt attagtgtag atggggcaac tttgtatgtt actacaagac cttgtgtttt
    78301 gtgtgcaaaa atgtgcataa atgcaggtat attgaaaatt gtttatgaag gtgattatcc
    78361 agatgatatg tctacagaat tattaaaaga agcaggaata gaactcataa aattttagtg
    78421 gataaaattt atatgacaat aaatgtcaaa ttatgacact gaaatacaat ggtatcaaat
    78481 gattaaaaga aagtatttga aaaatcatca gattagtagt aagatatatt ttataattaa
    78541 ataaatttat gagatagatt aattacatgt tatttatttt ataataatga gggatactat
    78601 ttatgagtaa aagaaataca catacagaaa ttgcaaagtt gtttagttta ataagtcaaa
    78661 taggattgat gattgtaata tcaatattag gatgtacttt cttaggaaaa tttttagata
    78721 gtaaattgaa tactactcca gttctgacaa taatattttt attgttgggt gttggaggtg
    78781 cttttagtgg tgtttataaa actctaatag tatatacacg aaggaagtga tatattggat
    78841 ataaaattac ttaaccaggt aaggtcagta acaaaaggta tagtaatatt tgatgtagtt
    78901 ttaattgtgc taatgttgat aacatcaaca ttaagtaaac cagtattatt aggacttata
    78961 tttggctcta taatagctat gttaaatttt agattattgg cgatatcact ggaaaaatca
    79021 gtgacatttc ctgctgggaa ggcacaagct tatgcatcag cacaatatgc tattagatta
    79081 tttataatgg cagttgttct aattgcatca gctacagtac cacatttaaa tataatcgga
    79141 acagcattgg gattattgag cacaaagttt gtcatattat caaaaaactt tataaataaa
    79201 ttaaagagga aggaggcata agtatgaatc aagcgcatta ttatctcata ggaaacttta
    79261 agattagtga aacaatacta gtaagttggg gaataatggc ctttttagcg atagcatctt
    79321 attttttgac tagaaactta aaaaaagttc caactagtaa agttcaaata ttcttagaat
    79381 ttgcagtagg tggtcttgct aaaatggtta cagatgttat gggtaaaaat acagtaaaaa
    79441 gaatgccata tatgatacca tacataggaa gcttgttttt atttttcgca tgttcaaatt
    79501 taagcccatt acttggatta aggtcaccaa caaccgactt agacacaact gctgcatggg
    79561 caatgattac attttttatg atttattttg caggagttaa atttaagggg ttatcatact
    79621 ttaaagaatt ggctcaacca cttgccataa tgttaccaat caatattata ggtgagttag
    79681 ctagaccaat atcattaagt ttccgtccat ttgggaatat tttaggtgga acaataataa
    79741 tgggtctgtt ttatcaactt ttaggtttta tatctagttt aataccagga ttatcaatac
    79801 caattggaga aatattaata ccagtcccat tacacttgta ctttgactta tttgcaggat
    79861 tgttacaagc atatatattt ataatgttaa caatggtatt tatatcaagt gcaggagagt
    79921 tagattaaga aatcaactta tattaagata aaaataaaat aatttaaaaa attaaagttt
    79981 taaggaggaa ataattatgg aaacagcaat agtagcggca gcatcagcaa taggagcagg
    80041 gatagcagtt gcaacaggta taggtgcagg tataggtcaa ggtatagcag cagctaaagc
    80101 tgcagaagca gtaggaaacc aaccagaagc taaaggtgat ataacatcaa ctttattact
    80161 aggagttgct atagcagagt cttcagcaat atatggtttg gttatatcaa taatacttct
    80221 attcgtaaat ccattcttta aatacttagg aatgtagatt tagtccttaa atattttgag
    80281 ataggtttat aggtaaatca tctcaaactt ataatcatga gatgaacttt atcatctcgt
    80341 gattataagt agttatgatt tatggaaagg aggataatta tggaaaaagc actagtaggt
    80401 ataacgtggg aatttgtgtt ccaaattgtt aatactttta taatattttt acttctgaga
    80461 aagcttttat tcaagccagt attaaatata attgaatcaa gagaaaatga tattaaaagt
    80521 gatttggcag aaggcgaaaa agcaaaaaat gaaggtttag ctctgaaaaa agaatatgag
    80581 tcaaaaatca attttgccaa ggatgaaggt caagaaatca taaaacaagc aactataaga
    80641 gcagagcaga aatcagatga tatagtaaat actgcaaaga aagatgctct tgatataaaa
    80701 gaaaaagcaa acaaagatat agaacaagaa agacaaaagg ttataaatga aatcaaaaat
    80761 gatatatcta acatagcact acttgcagca tctaaagtta ttgaaaaaga tttagataaa
    80821 tctaaacatg aagaattgat agaaaacttt ataaaagagg taggggaagc taaatgataa
    80881 atgtaatagc aaatagatat gccgaagccc tatttcaatt aggagaagaa gaaaattcaa
    80941 ctgatgtctt attcaaagaa ctagaaaaag tagtagatat gatgactaaa gttagtaaag
    81001 atttctacaa agttttaaaa tcaccattag tttcaaaatc agaaaagaaa aacttggtag
    81061 aaattatttt tagtaaggaa gtaagttcta acataaaaaa ctttttaaaa gttttagtag
    81121 ataaagatag aatatcatat ttagaagata tcgaattagc ttataaagaa ttattaaata
    81181 agaaaaacaa tgtaattgat ggagtagcaa taagtgctat tccaatgagt gaaacagata
    81241 ttaaagaact tgaagtcaaa ctttcaaata aatacaacaa aaatgttact atagaaaatg
    81301 ttgttgacaa gactatacta gggggagttc tagttagaat tggaaatgaa caaatagatg
    81361 gcactgtgaa aactcgtctt gataagatga aagaaaagct atctgaagta atatcttaag
    81421 aatggcggtg aacccatgaa cttaaaacct gaagaaataa gttctataat taaacagcaa
    81481 ataaaaaatt atgagaataa agttgagttg acagatacag gtagtgtttt aacagttggg
    81541 gatggtatag cgagtgtata tggattagaa aaagctatgt ctggtgagtt gctagagttc
    81601 ccaggtgaaa tatatggtat ggcacttaac ttggaagaag aagtagttgg agctgttatc
    81661 cttggagatg acagtgaaat aaaagaaggc gatatagtta agagaactgg aagaatagtt
    81721 gaagttccag ttggagaagc tttaataggt agagttgtaa actcactagg tcagccaata
    81781 gatggaaaag gacctatagc gtacacaaaa actagaccag ttgaatctga ggcacctggt
    81841 ataatagata gaaggtctgt ttatgaacca ttacaaacag gaatcaaatc aatagattca
    81901 atgataccaa ttggtagagg tcaaagagag ttaataatag gtgacagaca aactggtaaa
    81961 acatctatag ttattgacac tatactaaat caaaaaggaa aagatgttat ctgtatatat
    82021 gtagcaattg gacaaaaacg ttctactata gctcaattag ttagctcttt agaaaaaggt
    82081 ggagctttgg attatacaat agtagtttca gcaacagcat ctgaatcagc accacttcaa
    82141 tatatagcac catatgctgg agcagctatg ggtgaagaat ttatgtacaa tgggaaacat
    82201 gtacttatag tgtatgatga tttaagtaaa caagctgttg catatagaga aatgtcattg
    82261 ttacttagaa gaccaccagg acgtgaggca tatcctggag atgtattcta tttacattca
    82321 agattacttg aaagagcagc taagttatct gatgaattgg gtggaggttc aatgactgcc
    82381 ttgcctataa tagaaactca agctggtgac gtttctgcat atataccaac aaatgttata
    82441 tctataacag atggtcaaat atacttacaa ccagagttat tctattcagg tgtaagacca
    82501 gcagttgacc ctggtatatc agtatcaagg gttggtggtt ctgcgcaaat taaagcaatg
    82561 aaaaaagttg caggaacatt aaaacttgca tattcacaat atagagaact tgcagcattc
    82621 tcacagtttg gttctgactt agatgaagat actaaaaaga gacttgctca aggtgaaaga
    82681 atcgttgaga tattaaaaca aggtgagcat caaccaataa aagttgaaaa tcaagttatg
    82741 ataatatatg ctgttataaa taatcattta gaggatattc cgatagataa tatagctaga
    82801 tttgaatcag aattgtatgc atttgtagat aataattatc cggaaatatc aagaaaaata
    82861 ttaggtggag aagacttcac tcatgattta acagatgcaa taaatgaatt taaagaaaag
    82921 tttgttgttg aagtataatc ctttttaaaa aaggaggtaa attaattggc aggagctgga
    82981 atcaaagcaa tcaaaacacg tatgggaact gttgaaagta caaagcaaat aactaaagca
    83041 atggaattag ttgcatcttc taagcttaga aaagcaaaag aaaaggctga agattcaaga
    83101 gaatatttta atactttata caatacagta aaagatattg cccaaacaac acgtggaatt
    83161 agaaatgaat ttttaaaaga aagagaagta aaaaataaat gttatgtagt agtagctgga
    83221 gacagaggac ttgcaggtgg atacaattcc aatgtattaa aggaatctgt ctctcatatg
    83281 aaaggtaaaa aggaaacagt gattactgtt ggaaagaaag cacatgaatt tttttctaaa
    83341 agaaactaca atatagcaaa agatataaat agtgttgaag cttgtggata tgaagatgcg
    83401 acagaaattt cacaaactat tattgacctg tataaaaaag gtgaagtaga tgaagtttat
    83461 attgcttata caaaatttaa atctgcttta tcacaagaag ttgaaatttt gaaattgctt
    83521 ccattagtct ttgaaaaaga tacagacgaa caaggaatgg cagtaaaaga agaaaaacaa
    83581 aatagatcaa aagtacaata tataccatca gcagaagcag ttttaagtta tattgtacct
    83641 cagtttatat caggtacagt atatggtgga attttggaat cttttgcatc agagcaaggt
    83701 gctagaagaa ctgcaatgga atcagccaca gacaatgcga atgaaatgct atctaattta
    83761 gagttaagtt ataacagagc aagacagtca gctgtaactc aagaaataac agagatagtt
    83821 ggtggagttg aagctctaaa atagggaggt taggaaatgg caaatgtagg taaagtagta
    83881 cagattgtag gagctgtact agatgttaag tttgatagtg aacaaagcct acctaactta
    83941 ttaaatgcgt tagtaataaa gttaggagat aaagaaatag ttgcagaagt tgctcaacat
    84001 ataggtgatg atactgtaag atgtatagct atgagtgcaa cggacggact tgtaagaggt
    84061 atggaagttg tagatacagg agggccaata agtgtccctg ttggagatga gactttaggt
    84121 agaatattca atgttcttgg aaaaccagtt gatgggaaac cagctccaaa atctgcacct
    84181 aaattaccta tacatagacc agcaccggcg tatgatgaac tagaaactac agcagagata
    84241 ctagaaactg gtattaaggt agttgactta ctagctccat atctaaaagg tggaaaaata
    84301 ggtctatttg gaggagcagg agtaggaaag acagttctaa tacaagagct tataaacaat
    84361 attgctaagc aacatggtgg tatttctgta ttttcaggag taggagaaag aacaagagaa
    84421 ggtaacgacc tttatggcga aatgagtgag tctggagtta taaataaaac agctctagta
    84481 tttggtcaaa tgaatgaacc acctggagcg agaatgagag ttgctttaac tggacttaca
    84541 atggcagaac attttagaga tgagcaagga caagacgttt tacttttcgt tgataatata
    84601 ttccgtttca cacaagctgg ttcagaagtt tcagcacttc taggacgtat gccatcagct
    84661 gttggttatc agccaacatt agctactgaa atgggtgcac ttcaagagag aataacatca
    84721 actaagaaag gttcaataac atctgttcaa gcagtatatg taccagcaga tgacttaact
    84781 gacccagcac cagctacaac attctctcac ttagatgcaa aaacagtttt atcaagacaa
    84841 atatcatcac tgggtatata tccagcagtt gaccctcttg aatctacatc aaggattctt
    84901 gaccctagta tagtaggaaa agaacattat gaagtagcta gaggtgtaca atcaatactt
    84961 caaagatata aagaacttca agatataatt gctatacttg gtatggatga attatctgat
    85021 gaagataagt taatagttgc acgtgctaga aagatacaaa gattcttatc tcaatcattc
    85081 acagttgctg agcaatttac aggaaatcca ggtcaatatg ttcctgttaa agagactgta
    85141 agaggattta aagaaattct tgaagggaaa catgatgact taccagagtc agcattctta
    85201 tttgtaggaa caatagaaga cgctgttaga aaagcgaagg gaagtatgta gtcatggcca
    85261 gtgaattttc attgaaagtt gtaactccaa gtaatacttt ttatgatgga gatgttgaaa
    85321 tgatagtagt tagaactaca aaaggagaca aaggggtgtt gaaaggtcat ataccatttg
    85381 tttcaggtat acctgtagga actttaaaga taaaaaaaga gggaagtttt aaagaggcga
    85441 aaatagcaga aggattcatt aatgtaactc aagataaaac tgtcatattg acagaatctg
    85501 ctgagtggat ttaataaatt aatttagagt ataaaaagtc attttgacca aaaaaacttt
    85561 tgggtaaaat ggcttttttg tgttttggtg ttaataaaat aacgataaaa ttgtaagttt
    85621 ttagtgataa taaattcaca aaatgtttaa atttactgcc aaattatgtt gtaattaata
    85681 agataaagaa gatttagata gcttttatgt aagtgaaaaa gtcttcatat aagaaataca
    85741 tataaaattt ttataaaatt taaaaaattt gcttagttgt gtagtataat aaataatata
    85801 atagatttgt atataatatt cattacaaat aattttaaaa gttacagttt tattataatg
    85861 attattaaat taagataagt aacataaact aaataataat gtgtattatc gatagtaaat
    85921 ttataaattc attattaatt attgaaagaa gtattataaa ttttctggag gaacaatgaa
    85981 tatatttgat attggcgtag atataattga aattgataga ataagaaagg ctgtagacaa
    86041 aaataatagg tttttggaga agatatttac agatagagaa atagagtatt ttaattctaa
    86101 gaattttaaa gcagaaagta tagcaggtaa tttcgcagcc aaggaagcta taagtaaatc
    86161 tataggtaca ggaattagat tgtttaattt taaagatata gaagttctca gaaatgaaat
    86221 gggaaaacca atagtaaaaa cttataataa tctagcaaaa atgtgcatag attataatgt
    86281 tttagaaata aaagtttcaa tatcacattc aaaagattat gctatagcaa atgcgataac
    86341 cataattaag gactaaggag tgattacatt atgatggata aaacagcaaa agaaataatg
    86401 acaacagatg taatagtagc aaaacaagat gatagtatag cagatgtagc taatatgcta
    86461 attgcagaaa aaataggagg tctgccagtt gtagattcag agaatagagt tgttggaata
    86521 atttctgaaa cagatattct taaaaaagaa aaatatatag aagcacctct ctatataaat
    86581 ctattacaag gattgatttt cttagatgac ttaaaaaaag ttgaaaaaga tataaaacaa
    86641 gtagctgctt ataaggttgg agaacttatg tctaaagata taataaaggt tcatgaagat
    86701 gataagtttg atgatgtagc taatgtaatg attaaaaaat caataaacag agttcctgta
    86761 gtagatgatg acaacaaact taaaggtata atttgtagat atgatataat aaaagcttta
    86821 tacaatgaat aaactgaagt aggaggtgtg atgtgagaaa aaagtggacc atagtatgta
    86881 ttatgttctt ggcactggtt ataattgtaa taggatgcca aaaacgacag tccacaaaag
    86941 aagaagtata caaagatttt caaaagcaga tttctgatat gaattattac agttgtaaag
    87001 ctgaagtaga agtagttgga aataaaagcc cacacaatta tgttttaatt catacttata
    87061 aaaaaaccga taactataaa ttagaagtaa tttccccaaa acatttaaaa gggaaaagta
    87121 tagaatatca aggtgataaa atattagtta aaaatccaaa gattagtgat gtagtggagt
    87181 tacctaatac aggaaaaaat aatcaatatc tttttgtagg agactttata aaaaactatc
    87241 ttcaaaatga ggagatgaaa gtaaagttat ccaaaggaca tttggtttta gagactttta
    87301 ttcctgggga taataaatat tttaacaagc aagtattata tgtaaatgct gacacaaaaa
    87361 atcctgaaaa aatggaagtg ttagataaag agggagtgcc aagatttaca gtaaaataca
    87421 aagattttga atacagaaac taagggggac taaagacatg caaaaaataa cagtgcctac
    87481 atgggcagag ataaatctag ataacttaag atttaactta aataatatta aaaatttatt
    87541 agaagaagat attaagattt gtggagtaat aaaagctgat gcatatggac atggtgcagt
    87601 agaagttgca aaattgctag aaaaagaaaa agtagattac ttagcagtag caagaactgc
    87661 tgaaggaatt gaacttagac aaaatggcat aacacttcct attttgaact tgggatatac
    87721 tccagacgaa gcttttgaag attctataaa aaataaaata actatgacag tttattcttt
    87781 agaaacagca caaaagataa atgaaattgc aaaatcttta ggagaaaaag cctgtgttca
    87841 tgttaaaata gactcaggga tgactagaat aggtttccaa cctaatgagg agtcagtaca
    87901 ggaaataata gaattaaata aattagaata tatcgattta gaaggtatgt ttactcattt
    87961 tgctacagct gatgaagtaa gtaaagagta cacttataaa caagctaata attataaatt
    88021 tatgtctgat aaattagatg aggctggtgt aaaaatagct ataaaacatg tatcaaacag
    88081 tgcagctatt atggattgcc ctgatttaag attaaatatg gtaagagcag gaataatatt
    88141 atatggtcat tatccatctg atgatgtatt taaagataga ttagaattaa gaccagccat
    88201 gaaattaaaa tcaaaaatcg gacatataaa acaagttgaa ccaggtgtag gaataagtta
    88261 tggactaaaa tacacaacta caggtaaaga aacaatagct acagttccaa taggatacgc
    88321 agatggattt actagaatcc aaaaaaatcc aaaggttctt attaagggag aagtgtttga
    88381 tgtagttggt agaatatgta tggatcaaat aatggttaga attgacaaag atatagacat
    88441 aaaagttgga gatgaggtta tactatttgg agaaggcgaa gttacagctg agcgtatagc
    88501 taaagactta ggaactataa actatgaagt gttatgtatg atatcaagaa gagttgaccg
    88561 tgtttatatg gaaaataatg agcttgtaca aataaacagt tatttgctaa aataaaacca
    88621 tgatattata atctgggagg cgattttgtg tacgataaga gtaaagaaaa aatagaagtt
    88681 actttacctg actctctgat ttctgaagtt gatagtatag ttcagatgga gaacagtaac
    88741 agagaagatt ttgcaaaagc tgcttttcag ttctatataa ctcagaaaaa gaaaattcat
    88801 attaaagaat ctatgaaaac gggttataga gaaatgggtc aaattaatct gtcactagct
    88861 gaattaggta tgacagttga agaagtatct tctgatgata aaaatgaagg gaagattgcc
    88921 caaggtgagt actaattgtg aaataaagcg tggtgattta tattacgctg atttaagtcc
    88981 agttgttggt tcagagcagg gaggcgttag acctgttctg ataatacaaa atgatgtagg
    89041 taataaatat agtcctacag ttatagtatc agctataaca tctcagatta ataaagccaa
    89101 attacctact catgtagaga taagttccaa tgaatatggg cttaataaag actcagtaat
    89161 tcttttagag caaattagaa ctatcgataa aaagagactg agggaaaaaa ttggttgtct
    89221 agatgaaaat atgatgataa aagtagatga tggacttcaa ataagtttag gattgtttac
    89281 tttttaaaga gggatttgtc ttttattaga caatcccttt tttcttttaa atatctctta
    89341 aggtttaatt ttaatgtact atgttaataa aattaaaaat aacgcaaaaa aatagcataa
    89401 aagtgatata ataatatgga attgatataa aattacgaac tcaagggagg taattgcatg
    89461 agagaccata aaggattaaa tgagattgcg cgtatcataa gaagagatat agtttcaatg
    89521 atacatagag ccaaatctgg acatccagga gggtcattat cagtagttga aatacttact
    89581 gcgttatact ttgatgaaat gaatgtggat tcttcaaatc ctaaaatgga ggatagagat
    89641 agatttgtat tatcaaaagg acatgctgca ccagcattat atgctacatt ggctgaaaaa
    89701 ggctattttg ataaggaaga actgaatgga ttaaggaaga taggaaggat gttacaaggt
    89761 catccagaca tgaaaggcac gccaggtgtg gaaatatcta ctggttcttt aggtcaaggt
    89821 ttttcagtag catgtggtat ggctatggca tcaaagttgg ataatgctcc gtggaatgtg
    89881 tatactttgc ttggtgatgg tgaggttcaa gaaggaattg tttgggaagc tgctatgagt
    89941 gctgcacatt ataaacttga taatttaatt gcttttttag ataataatgg acttcaaata
    90001 gatggagata tagaaagcgt aatgagttta ggttcaatag ttgataaatt taaagctttt
    90061 ggttggaatg taattgagat agatggacat gattttgacc aaatatttgc ggctcttgat
    90121 atagctaaat caacagttaa aaaacctaca atgatagttg caaaaactat aaaaggcaaa
    90181 ggaatatctt ttatggaaaa tcaagcagga tggcatggga cagctccaag tgatgaggag
    90241 ctagaaaaag ccttgcttga attaggaggt gctgacaatg agtaaaatgg cgacgagaga
    90301 agcatatggt aaagcattag taaaattagg tcaaataaat gacaatgttg tagtattaga
    90361 tgcagattta tcaaagtcta caaaaacaca tgatttttac aagtcatttc cagatagatt
    90421 ttttaatatg ggtatagctg aacaaaattt aataggcgct gcttgtggat tatcaactgc
    90481 tggtaaaata ccttttgcaa gtacttttgc tatgtttgct acgggaaggg cttttgagat
    90541 tataagaaat tcagtttgtt atccaaagct aaatgtaaaa atatgtgcta ctcatgcagg
    90601 tcttactgtt ggagaggatg gagcatctca tgaaagtgtg gaagatattg ctataatgag
    90661 agctatacca aatatgacag ttcttgttcc agctgatgga gttgaaacag aaaaaattat
    90721 ttttgaaata gctaagtata atggacctgt ttatgttaga ttagggagaa gttcagtacc
    90781 agttttattt gatgaagatt ataaatttga aataggaaaa ggtacagtct taagagaagg
    90841 aaaggatgta tcaattatag cttgtggaat tatggtaaat gaagctctat tagcacaaga
    90901 aaagttgcaa gaagaaggta taaatgctag agttataaac atgtcctcga taaaacctat
    90961 agacaaagat ttaatattag aatcagcaaa agaaacgaat gctattgtaa ctgtagaaga
    91021 acatagcata attgggggat taggttctgc tgtaagtgag gtagtaggag agtcttgtcc
    91081 aaccattgta aagaaagttg gaattaaaga tacatttgga gaatctggta ctccaaatga
    91141 attgttaaaa aaatatgaat taacttgtga tgacataata aagactgtta aagaagctat
    91201 tattgcaaaa agaatgtaag agatataaat tattcatgtt tgttaataat ataatatatt
    91261 taaaagagag gtataaagtg ttatattaat ttatatctct ctttttttta tatgatatca
    91321 atatatcaaa a
//
LOCUS       DAEPLF010000002        73735 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEPLF010000002 DAEPLF010000000
VERSION     DAEPLF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 73735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.002"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            160..1542
                     /locus_tag="KR314_000094"
     CDS             160..1542
                     /locus_tag="KR314_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0326508.1"
                     /translation="MRKIWNKGHRIRASDKHLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     CTDWEHFSLLENDLTLSPYNFITILIATGACVLVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGSLKLMNNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGRLAQERQ
                     VMPEQQGKEN"
     gene            1546..1962
                     /locus_tag="KR314_000095"
     CDS             1546..1962
                     /locus_tag="KR314_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326509.1"
                     /translation="MEIKEINKIIQSEDKDEKSKSVKCICINYGDFLADCEGFVQKRY
                     HDFKCNPKHQFEKKADTILENAIHEKNFMPDLFLIRLNRKQSACNSQIDFVFELLDKS
                     FLETDPIRKSEISEETLNLCLSADVSFIMVYIGMNR"
     gene            2207..3403
                     /locus_tag="KR314_000096"
     CDS             2207..3403
                     /locus_tag="KR314_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBH0326510.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLALASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGRYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LVDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEQQAKLTEKHEMIIKQQTTPA
                     KDLVES"
     gene            3415..3549
                     /locus_tag="KR314_000097"
     CDS             3415..3549
                     /locus_tag="KR314_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBH0326511.1"
                     /translation="MWVLLKDFLLVSMGMGIGVVLMCILNIGKEADYEMKQLEESEDN
                     "
     gene            3550..3771
                     /locus_tag="KR314_000098"
     CDS             3550..3771
                     /locus_tag="KR314_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326512.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            3848..4321
                     /locus_tag="KR314_000099"
     CDS             3848..4321
                     /locus_tag="KR314_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0326513.1"
                     /translation="MDYIIREIKKEEYDLLNDFLYNAIYIPDGVEPPPKSIINCPELQ
                     EYVFEFGKRKDDRALVAEVQGNVVGAIWVRIMNDYGHIDNDTPSLAMSVFQKYRGQGI
                     GTSLLQQLLLVEKTSGYSKLSLSVQKNNYAVKMYKKAGFLVVDENSEEYIMTVNL"
     gene            4422..4718
                     /locus_tag="KR314_000100"
     CDS             4422..4718
                     /locus_tag="KR314_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0326514.1"
                     /translation="MSDIFKDMQVKVGCEYISDQPSYKRKVWQEMKRLNLADYEERQL
                     EDFSKYVFGMPYQTLKDVMKQQKGREEQCRKQGCWWKREEQLAKKQYHTGSTCR"
     gene            4637..5140
                     /locus_tag="KR314_000101"
     CDS             4637..5140
                     /locus_tag="KR314_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBH0326515.1"
                     /translation="MQETRVLVETRGTTGEETISYWFDLPIDVAEFEEKLGVGAESGD
                     YRIIEKVLAFANEVHEHTSVYQLNELDFMYRQLSSDMQEEYVSLLTVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            5158..5661
                     /locus_tag="KR314_000102"
     CDS             5158..5661
                     /locus_tag="KR314_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBH0326516.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNSMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            5771..6169
                     /locus_tag="KR314_000103"
     CDS             5771..6169
                     /locus_tag="KR314_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0326517.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            6147..8597
                     /locus_tag="KR314_000104"
     CDS             6147..8597
                     /locus_tag="KR314_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0326518.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLETNDFGYLIEHLYGRDGVAYEDYEYQLPKKKLRRE
                     TQIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSDSRGLLHVIDELRREDTPVSRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSSGDVAKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            8597..10807
                     /locus_tag="KR314_000105"
     CDS             8597..10807
                     /locus_tag="KR314_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBH0326519.1"
                     /translation="MKERIKGAFTKKKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTANGFSSSGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLFIFNIGITIFVFLLM
                     GIMMLSQILFIIFAMFLPISFLLSMIPSQENLAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISKDYPFFMVAFLQIVCFAGIFIKLGDLMNMFSLNAGDSQNMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRAERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQRRQNIADKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PATASTIPKPSVEKMQEVKRPATATTSKISEPVKTSVIKERPLSSGASDRKASQPIQP
                     AHRQNVEKVVSQETHQNYKAERTTKAQTFEQSKRTTEHTEKNRNLVMKKGQKKK"
     gene            10804..11811
                     /locus_tag="KR314_000106"
     CDS             10804..11811
                     /locus_tag="KR314_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBH0326520.1"
                     /translation="MKLKHIALIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITV
                     MNLSAEVLKHQPMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCQNQGIDDLNVAIQSYNYGGGYVGYVAGKGKKYTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLMQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGYADLSNSYWQQHLIGAGRVK
                     Q"
     gene            11828..12739
                     /locus_tag="KR314_000107"
     CDS             11828..12739
                     /locus_tag="KR314_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0326521.1"
                     /translation="MFKKNKKQTETIKEPKEKKVRTVKVGTHKKTVIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELCLQDTNGIENFVKNFAKSYYTWNNSKEAIEARTQA
                     ISGYLTKELQDLNVDTIRTDIPTSSTATNVIVWSIEQSGTNTFSATYEVDQQIKEGEK
                     TSNVKATYTVKVHIDADGDMVIVQNPTLAPAIEKSDYEPKTPEADASVDADTVNDATA
                     FLETFFKLYPTATEKELAYYVSGNVLEPIGRDYLYSELINPIFTKDGENVKVKVAVKF
                     LDNQTKATQVSQYELVLHKDSNWKIIG"
     gene            12852..13811
                     /locus_tag="KR314_000108"
     CDS             12852..13811
                     /locus_tag="KR314_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0326522.1"
                     /translation="MIGMDNRFNKSWYGDKAQLVDSDNDCVIIDYGCKGKGIITSYNL
                     YDGITLCFLDFYTDEIMPSQKFNPDIISIVHCQAGRYECEFSNHQMFYLSEGYFSIMG
                     TKHLPISFSFPLKKCYAFSLVIDKHALSADTQKILNDFCLDMDKISERLKLNKNGYLS
                     HADFELGHLFEEFYNAKDKESITYFRVKAIEFLYFINKLSGNEETEFRYFNKKYIQIT
                     KDVCKYMTEHLEEKVSLEQLAYEKGINLSLLYKVFAQVYGDTPYSYIKKYKMNIASNE
                     LRNSKRKINDIALSLGYSNASKFSIAFQSVYGILPKDYRKAEN"
     gene            13915..14448
                     /locus_tag="KR314_000109"
                     /pseudo
     CDS             13915..14448
                     /locus_tag="KR314_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896549.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14598..14885
                     /locus_tag="KR314_000110"
     CDS             14598..14885
                     /locus_tag="KR314_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015544968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326523.1"
                     /translation="MIVLKILAAPVVVALTLLAAVVSFLLCVAGAVCVVGCVVLTLLA
                     LVLFISGQTLGGVVFLVLAFLVSPYGIPAIADWLVARLHALNYTLRDFITG"
     gene            14961..16391
                     /locus_tag="KR314_000111"
     CDS             14961..16391
                     /locus_tag="KR314_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBH0326524.1"
                     /translation="MPTATTYFKKHKISKGETISQSLKGRFDYGKNPDKTEGGELISS
                     YACDHMTADAEFLLSKAKYKATTGREQRRDADVLCYQIRQSFKPGEITPEDANRVGYE
                     TAMRWTKGKYAFFVATHTDRQHIHNHIYYNSTSLDCAHKFRDFIGSARAVRRLSDRVC
                     LENDLSVIANPKLHSKGRFLHYGAWAGADRQPSYKERLRHAIRDALAKGPADFNAFLR
                     LMEEAGFTVKQGRGGAISFLVPGQQRATRLRSSTLGEGYDPEDIRAVIAGKRPLPEVG
                     VPAPAAPARVNLIVDIQERLRDRKGPAYERWAKIYNLKQMAAALQFMQEHGLTEYNQL
                     AAQTEEAVQRFHVLTGQLRQTEANLAHTSEVMGAVVQYAKTRPVFDGYKATKYSKKYL
                     AAHEAELADYRAAKAILNDLLDGARLPRMPELKEKRRTLAAQKKTLYADYRAAQQRMR
                     EAVAVKANIDHLLGLTDQRKNKEQER"
     gene            complement(16576..16932)
                     /locus_tag="KR314_000112"
     CDS             complement(16576..16932)
                     /locus_tag="KR314_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0326525.1"
                     /translation="MYQDERKLDFHALGREIKRKREAKGWTQEYLAQLVDRTPRSIMY
                     FENRGQHPSLNTFYQLVTLLDISVDDFFYPDKQNGQSDCRKHIDHLLNEMDEKELTII
                     EATAEGLKRARRTEEA"
     gene            17164..17352
                     /locus_tag="KR314_000113"
     CDS             17164..17352
                     /locus_tag="KR314_000113"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326526.1"
                     /translation="MAEIKEQLSQLNKMSNKSEALYHKVAFSIGISDSSLGVLYTLLD
                     AEAPCSQYDLCSEWSIPK"
     gene            17392..17640
                     /locus_tag="KR314_000114"
     CDS             17392..17640
                     /locus_tag="KR314_000114"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326527.1"
                     /translation="MVFLSPIPGTRNKKNIAFTEFGRDFAEKTVGILRMAELDALAEL
                     SPEERELYIRLNEKYNCRLIEKLYRIMDEVNQDRKDCD"
     gene            17663..18217
                     /gene="cfr(C)"
                     /locus_tag="KR314_000115"
                     /pseudo
     CDS             17663..18217
                     /gene="cfr(C)"
                     /locus_tag="KR314_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_111690898.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(8))-methyltransferase
                     Cfr(C)"
     gene            complement(18202..19860)
                     /locus_tag="KR314_000116"
                     /pseudo
     CDS             complement(18202..19860)
                     /locus_tag="KR314_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(19961..20113)
                     /locus_tag="KR314_000117"
     CDS             complement(19961..20113)
                     /locus_tag="KR314_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326528.1"
                     /translation="MDNDWNGLADLIANLITKYAGVLDLENLPNPTPVKNTEDKNKFD
                     IAKSTD"
     gene            complement(20602..21012)
                     /locus_tag="KR314_000118"
     CDS             complement(20602..21012)
                     /locus_tag="KR314_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0326529.1"
                     /translation="MAKEYYLYIRGQKVKVSEDIYIVYWREKEHEKYLEQVDRKNHLL
                     FFSALDHDGHFTDNIVDESVDVEKIVETQMMIEAVRSAITRLNAEERDIIERLYFNDE
                     TVRSVAKLKSITHPALIKRRNKILEKLKKFIEEL"
     gene            complement(21536..22855)
                     /locus_tag="KR314_000119"
     CDS             complement(21536..22855)
                     /locus_tag="KR314_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0326530.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLVFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLELYSKSSEIKAFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKTRLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKALQDEEFFIFKIKIFS"
     gene            complement(22852..23505)
                     /locus_tag="KR314_000120"
     CDS             complement(22852..23505)
                     /locus_tag="KR314_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0326531.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYNDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            complement(23603..24979)
                     /locus_tag="KR314_000121"
     CDS             complement(23603..24979)
                     /locus_tag="KR314_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0326532.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKIFQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSSKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFVGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIARQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFIIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(24979..25635)
                     /locus_tag="KR314_000122"
     CDS             complement(24979..25635)
                     /locus_tag="KR314_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0326533.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQKKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(25645..26922)
                     /locus_tag="KR314_000123"
     CDS             complement(25645..26922)
                     /locus_tag="KR314_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0326534.1"
                     /translation="MIKNAIAYITRKRNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIGENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNKSENNKIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKHIIKIMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMLNITTLGQ
                     SYLILISIIVLSVVFASSLILIKKPKEILSKIS"
     gene            complement(26941..27831)
                     /locus_tag="KR314_000124"
     CDS             complement(26941..27831)
                     /locus_tag="KR314_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0326535.1"
                     /translation="MDRIKNIINGSTKKDKYKIRDRFRHLFQSVWFLITNSYFEGFKT
                     GKIYGGNLKKICVPGMNCYSCPGAKGSCPIGSLQAVIGNSKYKISYYIVGILFFIGAL
                     VGRFVCGWLCPFGLIQDLLHKIPFFKKVETFKFDKHLRKLKYIILLVFVIILPLFLVD
                     ILGQGSPYFCKLICPIGMLEGGLPLVLLNKSMRNAIGFLYYWKGIILIVTLLLSIIIY
                     RPFCKYICPLGAIYSIFNPISIFRYRLDKDKCINCGKCKKVCQMNIDPVENCNHLECI
                     RCGRCKNACPVDAISYGVRK"
     gene            complement(27824..27919)
                     /locus_tag="KR314_000125"
     CDS             complement(27824..27919)
                     /locus_tag="KR314_000125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBH0326536.1"
                     /translation="MAMIYYGHSNGETKVVLDKAIRICMECIGIG"
     gene            complement(27998..29155)
                     /locus_tag="KR314_000126"
     CDS             complement(27998..29155)
                     /locus_tag="KR314_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBH0326537.1"
                     /translation="MKKIVSRGACLLLTLCLSLSTVGCKKSNESNKIKEGQSISSEEA
                     GMSTNEKDKNETFKPSDYTLKTKKEYVYEYLGLKFKLSDKFKKYMDDKKIAMLDDQSP
                     IDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELERIGTIGIFEKNTSEEKISK
                     ITKCDTHTKIGVSSDGKYDCYLSTNSGAESNLLDELKRTEIQIIDKKERPKNGFVLSE
                     KTDLENTEAFNKESVKDLRKLSTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEI
                     PDLVEVQNEMKSKGVNIVGVVTDTVDDNGENKEAIEKSKLIHEKTKASYPFLMPDKTN
                     FNGRLNGIQAMPETFFVDSNGNIVGDTYSGAKSSKEWKQVIEKELEKIKNK"
     gene            29489..29839
                     /gene="mobC"
                     /locus_tag="KR314_000127"
     CDS             29489..29839
                     /gene="mobC"
                     /locus_tag="KR314_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBH0326538.1"
                     /translation="MANRIRNERLEIKLTEEEKALFEEKRKLARCRNMSHFIRKCVLE
                     KEIYQVDLEPFRDLQGLLSNATSNINQIAKRVNSTGIIYKDDINDMKKQIEYFSKELW
                     QIHSLLLNRTSGGD"
     gene            29845..31176
                     /locus_tag="KR314_000128"
     CDS             29845..31176
                     /locus_tag="KR314_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBH0326539.1"
                     /translation="MAITKIHPIKSTLNLAISYIVNGKKTDEQILVSTHKCHQETAHT
                     QFLRTRNDVGTNGTVLARHLIQSFLPGEASPKMAHQIGLELCKKILKDEYEFVLSTHI
                     DKGHIHNHIIFNNVNMVTGKCYQSNKKSYHKIRYQSDKLCKENNLSVIDEFYESYKRK
                     YKTNGKSWYENEQSKRGTSWKSRLQFDIDRLIKQSKYWEEFLKKMAELGYEIKYGKHI
                     AFKPKDKTRFTRAKTIGEDYTEERLKERITENQSITTPPVKRRIGNIINMNANAKVKE
                     SKGYEYWATKHNLNTMAESVIFIREHGINSIKQLDKYIQKSAEERQDLQDKMKEIDKD
                     MQLLSDTMEQVHTVKKYRAYYKEYKANPSDKAFFEEHKAEITHYEIALSKLKKSYSKL
                     PNSKDILDKLNKLQEKKNTLIQEYSSTKSTMDELYQIRKNYGIYMGKEMER"
     gene            complement(31212..32018)
                     /locus_tag="KR314_000129"
     CDS             complement(31212..32018)
                     /locus_tag="KR314_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HBH0326540.1"
                     /translation="MEISKKELRKISRKFRILASNVMNSYYGEQNANLEEMINYIEQT
                     LLIKEYLHSLEYDVDGLDDLLNTISNSYGRKVLNLGNDSNKRTYLLYRAFSFITGNNL
                     PTYTFGWYYAGSNKYQDMAKAFGDRMVYPFVLEVENYMKDISTDMGYDENNALYNINV
                     NSSGVQVNIAEHGSTINADQENKFNTEEVIKAINNVEDLIKGMEDDNSKSILNQNLDN
                     IKNEIKQKHPKKEVLTTCLKSMHFITTSVALIPDLALGIKMLASLIGISI"
     gene            complement(32008..32469)
                     /locus_tag="KR314_000130"
     CDS             complement(32008..32469)
                     /locus_tag="KR314_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBH0326541.1"
                     /translation="MANDIKFEISVPADEEGYVQLKCPSCSEKFMLTVEDIEAESTID
                     IWCPNCGLTHENYLDDDIYELAERIVENQVAEMLNDFSKNLEKSFRNSKSIKFKRGKE
                     IKKEAELPIGRKTGDYEEKKYLCCGKTARISSIKKLEGSYCPFCGEMIDGD"
     gene            complement(32497..33564)
                     /locus_tag="KR314_000131"
     CDS             complement(32497..33564)
                     /locus_tag="KR314_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBH0326542.1"
                     /translation="MSGKLYIAAAGSGKTRKIIEDSLNTHKKILILTYTITNEQQIIS
                     RIKNKVGVIPNNITVSTWFSFLLKDGIRPYQGSRFKERIEGIIFVNGQNNTYVKKTSN
                     KYFAKDNRVYTDKLSECVVELNDKCNGKVFERISGMYDCIYVDEVQDMVGYDLEILKC
                     LIASRIEVIMVGDPRQCTYSTHSSKKYKKYSCGKIDEFLKNECRKLDVRIDETTLNCT
                     YRNNDIICKFANSLYPERTSTNFVRRAEDESDGVMIIDKADVEKYLQKFNAMQLRENK
                     RTSVNNEYPYYNFGESKGLEFQRVLIYPTQPMWDWIINNNTSLKEQSKARLYVAVTRA
                     ENSVVIVRKDARPCSLPVVKL"
     gene            complement(33561..35183)
                     /locus_tag="KR314_000132"
     CDS             complement(33561..35183)
                     /locus_tag="KR314_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0326543.1"
                     /translation="MSIRKLKIKNFKCFSDWFIVDFENGINILVGNNGTGKSTILEAI
                     NLVLTGTYHGKNIRNELTQYLFNKNIVENYIREVNEGVNVEPPKIIIEAYFDAEMPSF
                     LGDGNSEKTKDTPGICLSISFDEKYSNEYKMLCKNKITSLPIEYYEVSWMSFARENIT
                     ARSIPIKSAMIDTTSYTPTSGSDIYISRIVKNLLDDNEIINISQAHRLMKEQFSRDEN
                     IANINRKLTETSNNELTISVDFGTKTSWESSLVTQLNEIPYNNIGKGTQCIVKTQLAL
                     SDHKIENKQIVLLEEPECHLSYSNLNILLDKVNESVKEKQIIVTTHSSFVSNKLGLEN
                     LILLNRGKTLRLGDLSNETNLFFKRIAGYDTLRLLLSNKAILVEGDSDELIVQRAYMD
                     SHNGKLPINDGIDIISVGISFLRFLEIAKLLELRVSVITDNDGNIDALNRKYSDYLGD
                     NCCENINIYFDDSTHEYNGSLENYNYNTLEPCIYRSNSMSILNEILNKSYEDPDDLLK
                     YMKGHKTEVALKIFESDKAIEFPQYIKEGILI"
     gene            complement(35386..35616)
                     /locus_tag="KR314_000133"
     CDS             complement(35386..35616)
                     /locus_tag="KR314_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0326544.1"
                     /translation="MRVFSYKRLFKKLIDLDMTNNELMEKAKVSKSTFYKMKNGQNVT
                     TDVLLRICNALDCDIEDIMECVIIEDVRRMGV"
     gene            complement(35756..36415)
                     /locus_tag="KR314_000134"
     CDS             complement(35756..36415)
                     /locus_tag="KR314_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH0326545.1"
                     /translation="MDYKTMRDRVEDMLSDNHKDFVKAIISMEKGINDESALDKLYDA
                     YMDNDSLNLLHEEFDYMIEDLRGQGQIKDLLYVREEKDNLINIVGNIVGEVDVIEREN
                     KNGETFKVANFSVVSKDDEGNKLYHNCSVYGEKSDIPKDFKQGDFVKLFGQIRTSIDD
                     NGKEHSNIRILSSKLLKAKEQMKGQEEKKESILGAIKKYQAEDKEKPKEKKEASKEAE
                     R"
     gene            complement(36458..45187)
                     /locus_tag="KR314_000135"
     CDS             complement(36458..45187)
                     /locus_tag="KR314_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBH0326546.1"
                     /translation="MRINDFHNILELIKQDVLYNEAEYLKLLKVVGNNQRYDFRSQLS
                     IYDKNPEATACAKFDYWRERFNRTVMRGQKGIPILEDYGTYKKVDYIFDISQTVSKNR
                     DVNEVNLWRFDKEAHQDVLKEMIKSEGYEESESTLENIFSLSRLYGDEKIDTLMNELR
                     VADEDRISFTKFVRDSVSYAVASRFKLDYPMDYELLRENFQRFDSISLMSLGESVSDI
                     SGKIIDETIQKSKELELQKEVLIGKEAGYNKIKEELEEVEENVFRRDDQERNENERVL
                     RNGEYGRDNRKNQGEYAKQLGGTDGLYKEVSESDLRSDEAELPFTERGAEPLRDAFGS
                     IQGEEADRTPDGYSETGSRVYESREAKVDSSLEDRGREQSAVWGDDFSSQRNDHQGSS
                     GDLKDNTDKGIIEAEKASFSLPENSYGQISLTIPLTEKDIDTVLINGGNHDGGRLPVI
                     AEFSKGKSIEELGEYLKDTFKGGNGFYIDEREVSSWYSDKGIHLAYGTSAREDNTQIL
                     SWSDAASRINELLDRGEFATNVELLEAQDYERDRISESLWYLTHDLSEEGIEQGYFNS
                     FERGGGFPQETKRLSEALKNPEYLKETIKEYSRFLEGYKESRNVLRYHYHKVDSLYQK
                     LQELELQRKEYSTNLTELPKVKLFITEDEVLESLSRGSGVDRGKERITKFFKENHTLQ
                     EKANFLKDEYGIGGHSHVVSGAMGSDEWHDAKGFKLQKNDCNDVFLTWTSVAKHIDEL
                     LSKKLYFEEKETESTAEIEEVKEPQYYSKNDPGNLMPDEILERVPEIYAQEDVDLADK
                     QVHAAYIIPFRSNWTWYMTEYDRESGDAFGLVLGIEPEWGYFNLEELKELNAQRLILE
                     DFPKTFRELKDIELIKQMDEQELQFVFNGELSFEDKVELEAPEETEEATKRGTVQATL
                     FDYLKEREEVELNEKEDSLLEEFAVKAGDTVYFNHEEYKVREISKNEITGRYDLWLDP
                     VRSGNHQIPIVDFTDNEDLLNKISLERPNFIVGDEVKYKDKNHTITRFDDMRKNLNTV
                     TVKDNTEYFGGMITSFDVIPYRLESDLERVFENLTYKQPEQTTEETQIRKAEAHNFKI
                     TEETLPEKLSPSERLNNNLEAISMLNRVESGQRELDSTAQEVLARYVGWGGLSEVFDE
                     SKEGQWKEARAFLNENLSHAEYEAARESTLTSFYTPKTVIDGIYKTLSDMGFKQGNIL
                     EPSMGIGNFIGNIPDEMNKSKFYGIELDSVSGRIGKLLYPESEVQIKGLEETSFSNNF
                     FDAVIGNIPFGEYKVNDREYNKNNFLIHDYFFAKSIDKVRNGGIIAFITSSGTMDKKD
                     ESVRRYLAARAEFLGAIRLPNDTFKGIAGTEVTSDIIFLKKRDSIRERDEDWIHLAED
                     ENGLTYNKYFVDHPEQVLGTMREVSGRFGNTLACLPKENADLKELLTKASEEISKGST
                     YEEIELLDDEITSIPATDDVKNFSYTVIDDEVYYRENSLFIKKEITDKNKEKIKDYLE
                     LNTALKDVIYKQKEDYSDDEVKKAQEKLNEVYDNFSKKHGFINNLSNTRALKEDSNFP
                     LVSSIEILDEEENVKAKGDIFSKRTITKAKVIDHVDTSLEALVLSVSEKGYVDFEYME
                     SLTGKDRPTLIEELRGEIYLNIREEQNFYRPLSFNLEDGDLPFACANGSNSYKYGYVT
                     KDEYLSGNIRDKIAIVDSYLAKLRQTERELPHLGFVENGKEKELISYEMNRLEYQKAE
                     LTKVLPKELEASEINVRLGATWIPIKDIEKFIFETLKTPGYARWDIKVKFSNITSEWN
                     VEGKSRDKGNDLAEMTFGTSRVNAYKLIEDALNLKETKVFDQIINPDGSKTSVLNKKE
                     TMLAGQKQELLKEEFKNWIFNDQERRNRLVKLYNERFNSIRNREYDGSNLSFEGMNTE
                     IDLRPHQRNAIARSLYGGNTLLAHVVGSGKTFEMVASAMESKRLGMCSKSLFVVPNHL
                     TGQIGREFMQLYPSANIMVADKKDFEPKNRKRFIGKIATGEYDAVVIGHTQFEKIPMS
                     KEYQEKHIQDQINEIVNYVEEYKHDRNQNFTVKQLEKTKKKLETRLEKLNDDFKKDDV
                     ITFEELGVDMLFVDEAHNYKNLYLYTKMRNVAGIGQSEAFKSSDMFMKCRYMDEMTGG
                     KGIVFATGTPVSNSMTELYTMQRYLQYESLKKNHLEHFDSWASTFGETQSSFELSPEG
                     TGYRVKTRFSKFYNLPELMSMFKEVADIQTADMLNLPTPEANYEVIKTEPSEEQKEIL
                     KSLSERADDVRNRVVEPDVDNMLKITNDGKKLALDQRLINPLLPDNPDSKVNVCVKNV
                     FAIWDKTKENRSTQLLFSDMSTPKGDGEFNIYDDIREKLVAMGIPKEEIAFIHEANSD
                     KQKDELFAKVRKGEVRILMGSTQKMGAGTNVQNKLIALHDLDVPWRPADLEQRAGRIV
                     RQGNENEKVNIYRYVTENTFDAYLWQTIENKQKFISQIMTSKTPVRVAEDVDESSLNY
                     AEIKALATGDPKIKEKMDLDNEVTKLKMLEANYKSNRYRLEDKVAKNYPEEIARTEKL
                     IEAVKKDISNVEPKAEGEEKFTSITIAGEKITDKKLAGERLLEAISKVKINESKVIGK
                     YRNMDLEVSYNFFTNSHNFSLNGVAKHLGELGTSADGNITRLDNALEKMPEKLNMLEE
                     KLISTKEQLENAKEELKKPFEKADELKTKVLRLAELNKLLDMGEVEEKKNDNPLVEDV
                     KRAIIDFCNREYEENHSYDEFHTLYPDLKHIGIAYTNTPDERHGIQYELNLEEKTWTQ
                     YIDDTPIKTESFDYENKGENEALRNMKNEIELSTFSDLVYVDSEDLRVATGLDIDDEG
                     NFYDPFAKDLDNDGIPDRYDSDFKDSDYFESTYDVEDNLHSKEETTQKSDDKPSILGQ
                     IRAYQEESKTEEKQKIKEQEFLR"
     gene            complement(45270..46073)
                     /locus_tag="KR314_000136"
     CDS             complement(45270..46073)
                     /locus_tag="KR314_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004804477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HBH0326547.1"
                     /translation="MSKINVEGSEISIIAIDNRDYISLTDMVRNIENGSALIEKWLRN
                     KNTIEFLGIWEEMYNTNFNSPEFEGIKNEAGFNRFILSVKQWVEKTNSIGIVARAGRY
                     GGTYAHKDIAFEFASWVSPYFKLYLIKEFERLKEEEQKKLGWDIKRNLAKINYRIHTD
                     AIKNKLVPDKLSKEKINFIYASEADILNVSLFGITAKEWRDENPDLKGNIRDYADISQ
                     LVCLSNLENLNAVFINEGMSQSERLEKLNAIAIEQMKILSESESIKKLK"
     gene            complement(46158..47864)
                     /locus_tag="KR314_000137"
     CDS             complement(46158..47864)
                     /locus_tag="KR314_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBH0326548.1"
                     /translation="MKLVIAEKPSVAISIAKVIGANKKKDGYYEGNGYRVSWCVGHLI
                     QMANPDAYDEKYAKWNMADLPIIPSDYKYEVAKATKKQFNILKKLMNDKEVDTVINAC
                     DAGREGESIFRLVYNQVNCKKKMKRLWISSMEDSAIKEGFDNLTDGKDYDNLFESAQA
                     RAIADWLVGMNISRLYSCLYQQNYSVGRVQTPTLAMIVKRDDEIANYQKEKYYTVELS
                     MNGFTLSTDRIDDEVAAEQLISLVGDKIEITDVIQKEKITKPDLPFDLTTLQRECNKY
                     FGYSAKQTLDYTQSLYEKKLITYPRTDSRCLTEDMIVSTVNNILGKNDFDTERIKVVF
                     NSKKVTDHHAIIPTISSVKEDVSELPLSEAKVYFLISDKFHASVGYPLIENTTKIVAS
                     FDGFEFTSSGKVIKDEGFSKYLKEYKSKKSEDAVLPDVSVGDVLSVENKEVKEKFTQP
                     PKHFTEDTLLKSMEIAGNDALEKGVEVERKGLGTPATRAGIIENLIFKRFVERDKKNL
                     IATHKGISLVTIVADTFKSAETTAKWEMELSDIAQGKSSKKEFLDAIENEIKEAVLTY
                     RK"
     gene            complement(47959..49500)
                     /locus_tag="KR314_000138"
     CDS             complement(47959..49500)
                     /locus_tag="KR314_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBH0326549.1"
                     /translation="MKTSLKKNNKFWTVALAVIVSISCILGLWTVVYAQEQKSEAPTK
                     NEAVQTEVEVNVRYIFEDEKVFKEEKIKAEKGQLIDSGDLPMLPDDMKFIDEFLFYEV
                     KGDGKDEIIRKVAKMEVKDKETQTEEEKPKQDECTQTEDKGTQTELSKDDISKIEKEA
                     KELQDKLDKLNCELKDKDKLSDKQKNKIKDLEDEIDSLKEKMKKDKGNKDLSEDMKKE
                     IDKLTEKVKELEKKATETNKAPVTSQSVTPISPISGIKTSSGISPQTPQTSVSSTGKG
                     SSDSVSSGNTTKDTGKGKTEVKEKEKEVRYPNKLTAKAPANNSQDSSMGSASKSVNTN
                     KGVASSPSKARASVTENKDNANKEYPIHHGDSSDNKETDQYSADARQFITFQTKNGKT
                     FHLIINHDEDSENVMLLTEVSEDDLLNMVEKKEAPKQEVVKEEPVKEEVKPEKKDEKS
                     NLGTYIILLLVVGGALGAGYYFKVVKKKEDKELEALEEEDDDFFSESESEEEINDADE
                     VEDEE"
     gene            complement(49475..49714)
                     /locus_tag="KR314_000139"
     CDS             complement(49475..49714)
                     /locus_tag="KR314_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0326550.1"
                     /translation="MKKELTTVKNRIKKLKDKKALIDEELEPLFIREEELENEEIIAI
                     CRKNNITISDLIAKVNRQKAEMKKEKTNENQFEKE"
     gene            complement(49726..51858)
                     /locus_tag="KR314_000140"
     CDS             complement(49726..51858)
                     /locus_tag="KR314_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBH0326551.1"
                     /translation="MGKKLKKDFKERHKASLEREMFHSKTDTIPEESKLKHNDDYRGK
                     IVHEKERFQDKVHEKTSKVSADNEKSYGTSKRNEKYRTSDKGDVNSAIETGRSDYITE
                     VKDGKIYDPLGKDLDNDGIIDRYDNDFRDSNYFESTYDVEDNLSHKEKSLENSFKNHK
                     TQKSRYKRKNYSESLYTRKKDDAPKGNKADDKKAGKDAVSGKVYSSLSKDQKKKLKKD
                     MVKVSALSGLAKGSETVRDYLSHGSDENQGVEAGEKTADTSSKLIHGIKKYSDKKRAK
                     RGYDLTNKDYKIRKRKSKLEFRDAKKELKKTDEYKRASAYKRFQKKNQVKAAISRENK
                     SRLRDRIKEGLIGTLKSSKDMIIRKAKGLMLIFIGIIILGTFVINFAGTGMTGFMNST
                     SSVLTTSYLSKPNVLSEINQKFSDMEGELQSEVDYVKENYPGYDEYILNNTEYIGHNV
                     HELLSYITSRCGEVKSVSEVESILKELFESMYDLEYREEIEIRYRTVTETYTDEDGNE
                     YTESHEEPYEYKKLIVTLHKKEMDSIIRKVFANYPDNLKHYEALFLAQGNMREAFGNS
                     DLISANGGIGGGKEYEASTEVQKKIVNAAYITPSPGAGWCAMWVSQVYQNAGLGYIGG
                     NACDMYRNYTFTSDRSKLKVGMLVAVESSSSGSSAGLTYGHVGIYIGDGKVIDNIGRI
                     RVTTLDDWIATFCKHHPVGFGFPPNVKK"
     gene            complement(51862..54291)
                     /locus_tag="KR314_000141"
     CDS             complement(51862..54291)
                     /locus_tag="KR314_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0326552.1"
                     /translation="MNKKRKIQQIKLMQNEKALRSQKRDLKKSNCKSKKDKGGILDLI
                     FKKEPKRYTVEDTIPYLRLLKSGICQLDEKHFSKSIAFQDINYQLALDEDRDLIFNQF
                     ANFLNSFDPSISIEFSYINQLGRNEEMKSAIQIPDKKDGFDDIRFEFREMLKSQIVKG
                     NNGLKKSKYVTFTVEADNLEQATSKLERLEIDILSSLKSMGVRAESLNGEERLKILHD
                     VLNPNKTFKFSYKDLKKRESTKTYIVPDEFNFTPSRYFKFGKFIGATSHFQILASELS
                     DRMLSEFLDIDDNINISFHIRAIDQSEAIKMVKRKNTDIDKMKIEENKKAVRSGYDMD
                     ILPSDLITYGEDVKSLLKDLQTRDERMFVVSIVFMNFARTVQKLDNTIAQISSIANKH
                     NCKLKRLDHSQEQGLVSVLPLGVNKIEIDRGLTSSSTAVFMPFTTEELFINSSNSLYY
                     GLNALSHNLIMADRKKLKNPNGLILGTPGSGKSFSAKREMANAILVTDDDVIICDPEG
                     EYGNLVRQFKGEVIKVSSKSKDYLNPLDINMNYGDGDAPLKDKANFIMSMLELVVGGS
                     GLTAEEKSVIDRCLPKIYEKYFEHPTPDNMPILQDLYDMLKGQEEKVGKKLATEMEIY
                     VSGSLNVFNHRSNVDLNKKLLCFDIKELGSQLKKIGMLVIQDQVWNKVSQNRGNKATR
                     YYIDEFHLLLKDEQTASYSVEIWKRFRKWGGIPTGITQNVKDLLMSKEIENIFDNTDF
                     VLMLNQASGDREILARKLKISLPQLRYVTNSNEGEGLLFFGNTIVPFIDKFPKDTILY
                     QKMTTKPEEVR"
     gene            complement(54203..54592)
                     /locus_tag="KR314_000142"
     CDS             complement(54203..54592)
                     /locus_tag="KR314_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBH0326553.1"
                     /translation="MAYVPIPKDLNRVKTKVAFNLTKRQLIGFTLAGLVGIPVYLFMR
                     KFVPNDIAVIFLIVSTLPIFFITLFEKDGLTFEKYFKYIYLHKFYQPQKRVRKEVYLE
                     QEKKNSANKTHAKRKGIEKSKAGLKEK"
     gene            complement(54596..54856)
                     /locus_tag="KR314_000143"
     CDS             complement(54596..54856)
                     /locus_tag="KR314_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0326554.1"
                     /translation="MNKRKTVFIVVIGAGIMAIIDRIRLHNKVEELEEKTEDIGRCHN
                     DFYLIQQRYNKNTDEQLATIQEEIGSVYEHFEELSKGKEDGR"
     gene            complement(54874..55737)
                     /locus_tag="KR314_000144"
     CDS             complement(54874..55737)
                     /locus_tag="KR314_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326555.1"
                     /translation="MFGIFDKIEEFFKELLLGGIQANLESMFLDINDKVGAVATDVGK
                     TPMGWNGDVFAFIKSINDSVIIPIAGLIITAVLCIELINMVMQKNNMHDTDTFEFFKY
                     IIKMWIAVWLVSHAFEFSMAVFDVAQHMVNKAAGVINTSAVISGDQIVKMVDGLKDKG
                     LGELVMILFETSLIKVAIQVISIVIMLVVYGRMFEIYVYSSISAIPFATMGNKEWGQI
                     GTNYIKGLFAIGLQGLFLMVCLGIYAVLVKTIQITDIHTSTFTILGYAVLLGLMMLKS
                     GTLAKSVLNAH"
     gene            complement(55757..55972)
                     /locus_tag="KR314_000145"
     CDS             complement(55757..55972)
                     /locus_tag="KR314_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBH0326556.1"
                     /translation="MEFFAQAVNVLKILVMAVGAGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGLKLIPLLANVLK"
     gene            complement(55976..56287)
                     /locus_tag="KR314_000146"
     CDS             complement(55976..56287)
                     /locus_tag="KR314_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH0326557.1"
                     /translation="MKQEMININANLVAEPTFSSFEKEGEEVEVVNFTLVKKYGKGKE
                     YINCAAYGEKAEKAEDFEKGDLIHVFGYFKKREKEGKTYKNFVVKSYNKIEKKEENEE
                     E"
     gene            complement(56451..58235)
                     /locus_tag="KR314_000147"
     CDS             complement(56451..58235)
                     /locus_tag="KR314_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBH0326558.1"
                     /translation="MIDKILKDIKGLFKVQDKAKFLKQNIPYLAFFYVGNIFSHHVRA
                     YTGGDVIDKIFQGILELNTMSFLPSIHPTDILMGVVVAVLIKIIVYTKGKNAKKFRQG
                     KEYGSARWGTKKDIEPYMDEKFQNNILLTQTERLTMNGRPANPKYARNKNVLVIGGSG
                     SGKTRFYVKPNLMQMHSSYCVTDPKGTIVLECGKMLEDNGYEIKILNTINFKKSMKYN
                     PFAYLRSEKDILKLVQTIIANTKGEGEKSGEDFWVKAEKLYYTALIGYIFYEAPREEK
                     NFATLLDMIDASEVREDNETYMNPIDRLFEALEKKEPTHFAVKQYKKYKLAAGKTAKS
                     ILISCGARLAPFDIQELRDLMSDDELELDTLGDRKTALFVIISDTDDTFNFVVSIMYS
                     QLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIATIRSREISASIILQA
                     QSQLKAIYKDNADTIVGNCDSTLFLGGKEKTTLKELSETLGKETIDLYNTSETRSNQK
                     SFGLNYQKTGKELMSQDEITVMDGGKCIFQLRGVRPFLSDKYDITKHKNYKLLEDYDK
                     KNLFDIESYMKRKGKAKLNRETVITRMQ"
     gene            complement(58232..58717)
                     /locus_tag="KR314_000148"
     CDS             complement(58232..58717)
                     /locus_tag="KR314_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBH0326559.1"
                     /translation="MINEEIARKTLNMEINAGKVTGKVLLNLLKKLMKEAEKFGGLEK
                     LVNANGNEVKLKDMVKKGQLEEIPVEEAELKELKKELNRYGVKFSVMKNKETGKNSVF
                     FQAKDMKVMDKAFKHALSKSEKKAERKESIHKNIEKFKEMAKNSISKEKIKNKQKEQS
                     L"
     gene            complement(58719..59741)
                     /locus_tag="KR314_000149"
     CDS             complement(58719..59741)
                     /locus_tag="KR314_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0326560.1"
                     /translation="MYLFDEQPIVANKTLAREIGLNEALILQQINYWIEINKKSGKNY
                     HDGKYWTYNSIRAWQENDFDYMSFDTVKRTFSKLEKAGYLLVGNYNKDPRDKTKWYTI
                     NNDKLEELYMDMDLKKQKIQTEALKNSMPNALGQNAPMDKGKISQCNNADCTNAQVQN
                     ALMQKDNLHQPLPENTTNNTTNNTTNNSAYISIHPSIKEKNQLFSENMKGGRIDISYK
                     NIYQNCLEELRHQTGYYEHMSIGNKSVAEEFDEILKILADVMVLDDESFITINQTKIS
                     VQVVKERFRKLNSGHLEYLVGVLRENEYRIRNIRAFVLTAAYNAPSGIDAYYSALVSY
                     DMRGGY"
     gene            complement(59827..60108)
                     /locus_tag="KR314_000150"
     CDS             complement(59827..60108)
                     /locus_tag="KR314_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326561.1"
                     /translation="MRWILKIILFPISIALSILIAFLSFLLGIGTAILYLLMMFCIFG
                     AIASFLQKEVTIGIEALILGFLLSPYGIPMVGTAVIAVLTGVKEAIRSI"
     gene            complement(60127..60438)
                     /locus_tag="KR314_000151"
     CDS             complement(60127..60438)
                     /locus_tag="KR314_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009537682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HBH0326562.1"
                     /translation="MVETVALLSKLDVDKHIDVEIKLDELDLTSAESKATYAQIKEYV
                     LEKFGLKVSTLYIAQIKKKCGIELREHYNKSKKDNQAIPQCTPEKEEAIMDALRHFKM
                     I"
     gene            60425..61132
                     /locus_tag="KR314_000152"
                     /pseudo
     CDS             60425..61132
                     /locus_tag="KR314_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(8))-methyltransferase
                     ClbB"
     gene            61529..61951
                     /locus_tag="KR314_000153"
     CDS             61529..61951
                     /locus_tag="KR314_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0326563.1"
                     /translation="MAIINLKEFYPWYTHDEYIEVSDEVAAELRADKLYEAAHQRRIV
                     RNKAQYSLDCNDGIEYSACLHEPTPQELLERMDQFCTLWNALNTLPEIQGRRVDACVI
                     LGKSYCEVAKAEGVNESAVRRSVERGLKQMKKHLKDTL"
     gene            62282..62482
                     /locus_tag="KR314_000154"
     CDS             62282..62482
                     /locus_tag="KR314_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326564.1"
                     /translation="MSCDPVLAAMLARPWNNNACRGYVIYAMENCGFSPTDIRRVVAE
                     LHEVFDFCGLEEAQQHFENGPY"
     gene            62616..62762
                     /locus_tag="KR314_000155"
     CDS             62616..62762
                     /locus_tag="KR314_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326565.1"
                     /translation="MEVPVPTKEEYTYQVGKIQFIVTPVYKDKGESMRDILLKLMLAD
                     LEPA"
     gene            62827..64554
                     /locus_tag="KR314_000156"
     CDS             62827..64554
                     /locus_tag="KR314_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0326566.1"
                     /translation="MFDCWKEDILKQSSKKTALGTAALYCRLSRDDNMDNESNSISNQ
                     KKILQKAAKDKGYTDTIFFVDDGITGTTMKRPGFQKMIAAIEAGYISAVFVKDLSRLG
                     RNYIEVGKLTEEFFPLHDVRLVAVSDGVDSDEGEDDFTPFKNIMNEYYAKDISKKRRI
                     VNKMKGNAGIPLSPPPYGYIKNPDDPRFWVVDPVAADVVRRIYRMALEGYGLAETAAA
                     LGADGIVNPTYYWRSKGTSRGGSKSTLEPTKWGHTTIKKILTTQEYCGDVINFKSYSK
                     SYKMKRRIENPEENRAIFLNVHEAIIDRPTWEKVQALKAGTRRKRPTVTQEPSAFSGV
                     MKCPECGGNLNFHFNQNNHDIKFFSCQNHNSGLRKCSSTHYIRLDFLEQVVLYEVHRL
                     ACFANEYENDFIKAMVGRSAKVAENERVRKKRELDGLLARDRELDTLFERLYEDNVSG
                     KIDDARFAKMAKRYEQEQGENAGRIKALRLEVKKLEEKRMDVDDFLETVRHYTNAAKI
                     TKRMVAELIDHIEVYPAVKEDGVTNQRVTIHYNCIGAFEVPDRRKIPERDILLETRKG
                     VALSYAPAV"
     gene            64569..64913
                     /locus_tag="KR314_000157"
     CDS             64569..64913
                     /locus_tag="KR314_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0326567.1"
                     /translation="MRVFIYCRVARDDTFALEHQRFLLQLYAKRAGYTIIGAADEYGS
                     GLTLDRPGMGKVTQAVLAGEVDVVLVHSLTRIGREWGMTQSYIDQLTRHKVKLLCIKE
                     RCLFSEKGVIHF"
     gene            65000..66733
                     /locus_tag="KR314_000158"
     CDS             65000..66733
                     /locus_tag="KR314_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0326568.1"
                     /translation="MKKLKEILFLSDKGYTDLKKAIIACTITNLAMLLPFCITVMVFS
                     ELLNPFLGQELDWQNIWLLWGAGIISAIIVFLASKNDYRKTYIASYKESNVTRLRIAE
                     HLRKLPMSFFNTKDLSELTTNMMSDCSSMESMLSSTIPPLIANGISVTLTCVLLALFD
                     WRLALCVFITLPIAFLVIWCSRKYQIKLFEKQVETKLNASSQVQEYLEGIKIIKSCNL
                     SGVHFRTLNRALLEMKKIAVKVEMVVGIFMSSASMILQAGIGITIFVGTILLVNGQIE
                     LLPLLMFFLIVTKIYGPILAILSQLTTLLNLNVVTERMKTLLTTPAMSGKEETPQTYN
                     IKLNDVSFAYNNEDVIHNVSCTIPQGSITALVGPSGSGKSTIAKLIARFWDIQSGSIT
                     IGNKNIKTINPENLMEKMSFVFQDVTLFNDTVFNNIQMGNPNATKEQVYKAAKVAYCD
                     EFVRNLPNGYDTILGENGSTLSGGERQRISIARALLKDSPIILLDEATASLDPENEVF
                     VQKALAHLIKGKTVIMIAHRLRTVVDADQILVMENGKIVEHGTHNELMERKGLYHKLY
                     SIQQESLGWSV"
     gene            66860..67084
                     /locus_tag="KR314_000159"
     CDS             66860..67084
                     /locus_tag="KR314_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326569.1"
                     /translation="MIGLKKRDIKKALKAGAKAGGVSLSDVRADIEATIDEAMNSTDP
                     EVQANFKKYFGNKRPTSEEYIYKITQKTKV"
     gene            67365..67793
                     /locus_tag="KR314_000160"
     CDS             67365..67793
                     /locus_tag="KR314_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0326570.1"
                     /translation="MKPSNFQRTIQCQFDCKLKRVVKGIVRNYRKELKRRRNKEISYC
                     ELPEIVVEKLAVWDEYESDYTAFDVCGIEVRVLDDDLAEAIKYLSEKDREILLMYFFL
                     GMSDTEIGDRLKINRSTSFRSRKNSLEEIKKKLKENMNDE"
     gene            67786..68025
                     /locus_tag="KR314_000161"
     CDS             67786..68025
                     /locus_tag="KR314_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0326571.1"
                     /translation="MNNTRPSFYLISSAVDGNVNSIEKILAFYDPYISKCCLRPFYDR
                     YGNVCIVVDMELKGRIREALIKMMLDFDIPLETEE"
     gene            68401..68604
                     /locus_tag="KR314_000162"
     CDS             68401..68604
                     /locus_tag="KR314_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBH0326572.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENLDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            68683..69873
                     /locus_tag="KR314_000163"
     CDS             68683..69873
                     /locus_tag="KR314_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH0326573.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYSWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLNDGIDTVGGKMTVCQLYDKKNSQRKNIKRA
                     TEKGRQYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSEKGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEQKVILTPEQEERLLAFMEKDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRVLNIDHQLLKDSEMGYYIETPKTKNGKRELPLTE
                     RAYQAIQRILKNRGKAQPLIVGGYSNFLFLNREGLPKVAGNYEGMVRGLIKKYNKYHT
                     DKLPNITPHSFRHTYCTNMANRGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            complement(70028..71449)
                     /locus_tag="KR314_000164"
     CDS             complement(70028..71449)
                     /locus_tag="KR314_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBH0326574.1"
                     /translation="MIKLSKFPKDFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKHYESDMTVDKIKFAMKDNNGYYPKRNGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKDKVKYWIVINQINLMYHESFNSVAFCVDQVDDVEEAKYQ
                     AIHHQFMATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRIQYFFT
                     DVSFRGEYPAYIKRFFKENNINIVMEEGDDKVLKENTMDFLALSYYYSTTVSASKNTM
                     NPIDNVKNQYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYKNLLNF"
     gene            complement(71471..71824)
                     /locus_tag="KR314_000165"
     CDS             complement(71471..71824)
                     /locus_tag="KR314_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0326575.1"
                     /translation="MTTEEHVIDEELVEVAMQIILRAGEARTEIKHALNDLERFDYKN
                     ADLKLAKAKEFMTEAHRAQTNIIQGEASGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(71853..73100)
                     /locus_tag="KR314_000166"
     CDS             complement(71853..73100)
                     /locus_tag="KR314_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0326576.1"
                     /translation="MGRLFMNKFIDLLSEKFTPVVNKITKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVALVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDSMLPIAVIMFIGWGLIYGLNFDMFEIIVRIFKPVSDIAQTLPGFV
                     LLNFLVVFLYSLGISGWVLSPITYAIQLSAIAANAAAVASGGLATNIYTYEVVQTGWI
                     TLGGWGATLPLVLMMCLSRAGRIKAIGRACIVPSIMNINEPVMFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYLPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEELKLESQEA"
     gene            complement(73108..73425)
                     /locus_tag="KR314_000167"
     CDS             complement(73108..73425)
                     /locus_tag="KR314_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0326577.1"
                     /translation="MKNIKVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLPEIDCLLLGPHLDYMKDEIQKKADEYNVPVACISKTAYGRLDGVKALEEALELI
                     LNK"
BASE COUNT        21695 a        14942 c        12397 g        24701 t
ORIGIN      
        1 ttggactgtt atttttggct attggtgtaa ttggcattat atgtgcgacc ttaatataac
       61 taaaaatgca gatttatgat tatatcttgc aacatttaca aagcagttag tctatacgat
      121 tgactgcttt tttcataccc agaaaggagc gaatgattta tgcgtaagat ttggaataaa
      181 ggacatcgta tcagagccag cgacaagcac cttgtctacc acttttccat agggacgctt
      241 ctgtttgtgt tcgtggcggt tcttctgcta ctgaatatca aacagctcat gtgtaccgat
      301 tgggagcatt tcagcttgtt agaaaacgac ttgacgctct ccccttacaa cttcataacc
      361 atactgatag cgactggtgc ttgtgtattg gtcgcttttc tgtattaccg cttctgttac
      421 gacagtttca agaagctcct gcaccgtcaa aagctggcaa gaatgatact ggaaaataag
      481 tggtatgaag ccgataccgt acaagacagc ggttttttta ctgacctgca aagcagatca
      541 agggaaaaaa tcgtctggtt tccaaagatt tattatcaaa tggaaaaggg actgcttcat
      601 atccgctgtg aaatcacatt aggaaaatat caagaccagc ttttacggtt agaggataaa
      661 ttggaaagtg gcttgtattg cgagctgacc gacaagaccc tgcatgacgg ctatatcgaa
      721 tataccctgc tttatgatat gatagcgaac cgcattacca ttgatgaagt acgggcagaa
      781 aacggcagtc tgaaactgat gaacaatctt gtctgggaat atgacgcact ccctcacgct
      841 ctgattgctg gtggaacggg tggcggtaaa acctatttcc tgctgacgct cattgaagcc
      901 ttactgcata ccaacgctgt cctttatatc ttagacccga agaacgctga ccttgcagac
      961 ttagggacag ttatgggaaa tgtgtatcac accaaagaag aaatgataga ttgcgtcaat
     1021 gccttttatg agggcatggt acagcgaagt gaggaaatga agcgacaccc gaactataag
     1081 actggcgaaa actacgccta tctgggactt ccaccctgct ttcttatctt tgatgaatat
     1141 gtagcatttt ttgaaatgct tgggacgaaa gaaagcgtga gcctgcttag ccagttaaag
     1201 aaaatcgtta tgttgggacg acaagcagga tatttcctta tcgttgcctg ccagcgtcca
     1261 gacgcaaagt atttttcaga cggtatcaga gataacttca atttccgtgt gggacttggg
     1321 cgtatcagtg aattaggtta cggtatgctg tttggttcag acgtgaaaaa gcagtttttc
     1381 cagaagcgta tcaaggggcg tggttattgt gatgtgggaa caagcgtgat aagtgaattt
     1441 tacacgcctt tagtcccgaa aggacatgat tttttgcaga ctatcggcag gcttgcacaa
     1501 gaaagacaag ttatgccaga acaacaagga aaggagaact aactcatgga aataaaagaa
     1561 atcaataaaa taatacaatc agaagataaa gacgagaaat ctaagagcgt aaaatgtatc
     1621 tgtatcaatt atggagattt tctagccgat tgcgagggat ttgtgcagaa acgttatcac
     1681 gattttaaat gtaatccaaa acatcagttt gagaaaaaag ctgatactat tcttgaaaat
     1741 gcaattcacg aaaaaaactt catgcccgat ttgttcttga tacgcttaaa tagaaaacag
     1801 tcggcttgca acagccagat tgattttgtt tttgaattgc ttgataagtc ttttctggaa
     1861 acagacccta tccgtaaaag tgaaatatca gaagaaacat taaatttatg tttatccgct
     1921 gatgtttcat tcatcatggt ttacattggt atgaacaggt aggataagtt ctgcctgcac
     1981 aacggcagga acggtcggcg gagcaaatac gaccgtggaa gccgttttgc agagcagggc
     2041 ttgtccctgc tctgctgatg aacccctcgg tatctaacag aggggtacgt ttgcaaataa
     2101 acaatagaca aaaaacatag gaaacataag gcttctgaca tggtttccta gtaaaaatcg
     2161 gctgtgaagt cgccttaaaa aacttcacac tttacagatt gggggtatgg ttctgaatga
     2221 agaacaatgg ataaaagaat tacgggagaa acggattgct tatggtatct cacaaggcag
     2281 gctggcgttg gcttctggta tcacaaggga atatctcaac aagatagaaa gtggaaaaat
     2341 gaagccgtca aaagaacttc tggaaactct gcataaagaa ctggcaaggt tcaatccaga
     2401 agcaccgctt accatgctgt ttgattatgt gaaaatccgt tttcccacgc tggatataca
     2461 gcacatcatc aaagacatat taaaactgaa tatcaactat atgctccatg aagattacgg
     2521 gcgttacagt tatacggagc attattcttt aggggacatc tttatctata cgtcggctga
     2581 cgaagaaaaa ggtgtccttt tagagttaaa ggggcgtggg tgcaggcagt ttgaaagtta
     2641 cctgctggca cagcaaagaa gctggtatga ctttctcatg gacgcactcg tagacggtgg
     2701 cgtgatgaag cgtatcgacc ttgctatcaa cgaccatacg ggcattttgg atattccaga
     2761 gcttgcggaa aaatgcagga aacgggaata tatcggaaag tccagaagct ataagtttta
     2821 ccagtcgggc gagcttatca agcacagaga ggacgacaga gaatatatgg gacgtaccct
     2881 ttatcttggt tcgctgaaat cagatgtgta tttctgtatc tatgaaaagg actatgagca
     2941 gtacgtcaag ctggggacac ctctggaaga agccgacatt atcaaccgtt ttgagatacg
     3001 gcttcggaat gaacgagcct attatgcagt acgagatttg ctgacctatt atgacgcaga
     3061 gcagaccgcc ttttctatca tcaaccagta tgtgcggttt gttgatgaag aaccagacaa
     3121 gcgaaaaaat gactggaaac tcaatgaccg ctgggcgtgg tttatcggcg ataacagaca
     3181 gagcttgaag ctgacgacaa agccagagcc ttatacctta gaccgtacat tgcggtgggt
     3241 acaaaggcag gtagcaccaa ccttgaaaat gctgaaaaag attgataagg gaaacggtac
     3301 agactacatg gaaacaatcg aacagcaggc aaagctcaca gaaaagcatg aaatgataat
     3361 caaacagcag acgacccctg caaaagattt agtagaaagt taggagtgat aaaaatgtgg
     3421 gtattattaa aagacttcct gctggtatct atgggaatgg gtatcggtgt agtcttgatg
     3481 tgtattttga atatcggcaa agaagctgac tatgaaatga aacaattaga agaaagcgag
     3541 gacaattaaa tgaatttcgg acaaaatttg tatcaatggt ttttatcaaa cgcacagagc
     3601 ttagtgctta tggcgattgt ggttatcggt atctacttag ggtttaaacg tgagttttca
     3661 aaacttattg gcttcttggt agttgccttg attgctgtcg gtttggtatt caatgctggc
     3721 ggtgtgaaag atgtactgtt agagctgttc aataagatta ttggtgcata aaattttatt
     3781 gtggtactga tatgcgaatg atataatcgg ggtgtgaaaa aagataaaat agattaggag
     3841 ttaagctatg gattatatta tacgagaaat aaaaaaggaa gaatatgatt tactcaatga
     3901 ctttttgtat aatgcaattt atattccaga cggtgttgag ccaccaccta aatcaatcat
     3961 taactgccca gaattgcaag aatatgtttt tgaattcgga aagcgaaaag atgatagggc
     4021 tttagttgcg gaagttcaag gaaatgttgt tggtgctatt tgggtacgaa taatgaacga
     4081 ttacggacac attgacaatg atacaccgtc ccttgctatg tctgtttttc aaaagtatag
     4141 aggacaaggg attggaactt cattattgca acagttattg ttggtggaaa aaacttctgg
     4201 atattcaaaa ctttccttat ctgttcaaaa aaataattat gcagtaaaga tgtataagaa
     4261 agcaggtttt cttgttgttg atgaaaatag cgaagaatat attatgactg taaatctata
     4321 acagataatc tagtttacaa tttcatatcc atacacacaa agcagttagt cttaggattg
     4381 actgcttttt tcttacctaa atttcagatt ggagtgatga aatgagcgat atttttaagg
     4441 atatgcaggt aaaggttggc tgtgaatata tttccgacca gccctcttac aagcgtaagg
     4501 tgtggcagga aatgaaacgg ctgaaccttg ccgactatga agaaagacag ttagaagatt
     4561 tttcaaagta tgtgtttggt atgccgtacc agaccttaaa agatgtgatg aaacaacaga
     4621 aaggacgtga ggaacaatgc aggaaacaag ggtgctggtg gaaacgagag gaacaactgg
     4681 cgaagaaaca atatcatact ggttcgacct gccgatagat gttgccgagt ttgaagaaaa
     4741 gttaggtgtc ggtgcagaaa gtggggatta ccgcattatt gaaaaggtgt tggcttttgc
     4801 taatgaagtt cacgaacata ccagcgtgta tcagctcaac gaattagatt ttatgtaccg
     4861 ccagctttca agcgatatgc aggaagaata tgtatcactt cttacggtgt ttgagaattt
     4921 agaagcactt tatatttgca ggaacgtgat tacggtttat cccgactgca aaagcatgat
     4981 agatgttgca aggcaaaagc tgatgaacga cccgacgttc aaacatttat ccgaggactg
     5041 tcaagaatat tactttgact ttgaagccta cgcttctcac ttgcaggaac acgggaaatt
     5101 tttagtaacg gaacacggta tctttgaact gccagagtag gaaatgaggt ggtgcttatg
     5161 attgatgata tggcggttta tattgctaat cttggcaaat acaacgaggg ctatttagtc
     5221 ggtgcgtggt tcacgttccc cattgacgag gaagatgtga aagaaaaaat cggcttgaat
     5281 gaacagtatg aggaatacgc aatccatgat accgataact ttcccattgc gattggcgag
     5341 tatgtttcca ttgaagaact gaactctatg tatgaaatga tagaggaact tcccgactat
     5401 atcgtagagt gtctggacga atttatcagc cactacggga cgctggaaga agtcgtggaa
     5461 cacaaggacg atatttatta ttatccagac tgtgaaacca tgacagacgt tgcctactac
     5521 tacatagacg aattgcaggc acttggcgac attccaccca gcttacagaa ctacattgac
     5581 tatgaagcct acgggcgaga tttggatatg ggcggttgct tcattgaaac aagccgaggt
     5641 atgtgcgaga taccatatta acgctggaag atcagcgaca agcattgttg ctactgacag
     5701 cgtatttctc ttttaaggga cagtctgaaa catggctgtc cttttctatt ttgaaaggag
     5761 ctgaaagaac ttgaaaaaga ttaaatctta tacgggtatc tggaacgtgg aaaaagtctt
     5821 gtatgcaatt aatgacttta acttgccctt tcccgttacc tttacgcaga ttacatggtt
     5881 tgtgattaca gaatttatca tcattctgtt tggggatatt cccccacttt ccatgattga
     5941 gggagcattt ctcaaatact tcggtattcc cgttgctctc acttggttta tgtcgcagaa
     6001 aacttttgac ggaaagaagc cgtacagctt tttgaaatca cagataacct atgccctgcg
     6061 accaaaaatc acttatgcag gaaaagccgt aaaactgcat aagcagatac tcaatgaaac
     6121 aatcacggca gtaaggagtg tgaactatgt tcccgataaa atatattgac aataaccttg
     6181 tctggaacaa ggacaatgag gtgtttgcct attatgagct gataccgtac aactattctt
     6241 tcttatccgc agagcagaaa tttatcgtgc atgacagctt tcgacagctc attgcacagt
     6301 cccgtgaggg taaaattcat gcgttgcaga ttgccacaga aagctcaatc cgaagtatgc
     6361 aggagcagtc aaaaaagctg gtaactggaa aattaaagga agttgcctat cagaagatag
     6421 acgaacagac cgaagcgtta gtatctatga ttggggataa tcaagtggac taccgctttt
     6481 ttcttggctt taagctcatg gttacggaag aacagctcaa tctgaagaac atcaaaaaat
     6541 cggcgtggct gacgttcacg gaatttctcc atgaagtgaa ccacacgctg atgaatgact
     6601 ttgtttccat gccaaatgat gaaatcaacc gctacatgaa aatggaaaag ttactggaaa
     6661 acaaaatatc ccgtcgtttc aaggtgcgac gcttggaaac aaacgacttc gggtatctca
     6721 tagaacatct ttacggtagg gacggtgtcg cttatgaaga ttatgagtat cagttaccaa
     6781 agaagaaatt gaggagagaa acgcagataa aatactacga ccttattcgt ccgacaagat
     6841 gtgtgattga ggaaagccaa aggtatttac gattggaaca tgaggacaag gaaagctatg
     6901 tgtcctactt taccgtcaat gcgattgtgg gcgagctgga ttttccgtcc agtgaaatct
     6961 tctatttcca gcaacagcaa ttcacattcc ccgttgatac ttctatgaat gtagaaatcg
     7021 tggaaaatcg gaaagcatta acgacagtcc gcaacaagaa aaaggaattg aaagaccttg
     7081 acaatcacgc ttatcaagca ggaagtgaaa ccagctcaaa tgtggtggac gcattagata
     7141 gcgtggacga gctggaaacg gacttagacc agagcaaaga aagtatgtat aagttaagct
     7201 acgtcatacg ggtgtctgca cccgatcttg acgagctgaa acgccgttgt gatgaagtca
     7261 aggactttta cgacgacctc aatgtaaaac tggtgcgtcc tgctggggat atgctggggc
     7321 ttcattcaga atttcttcct gccagcaagc gatatatcaa tgattatgtg cagtatgtaa
     7381 aatcagattt tctagcaggg cttggttttg gtgctaccca gcagttagga gaaactacgg
     7441 gtatctatat gggctattct gttgatacgg gaagaaatgt gtacctgcaa ccgtcccttg
     7501 ccagtcaagg tgtaaaaggt acagttacca acgctttagc gtctgctttt gtcggttcgc
     7561 ttggcggtgg gaaatcgttc tgtaacaatc ttctggtgta ttattcggtg ctgtttggcg
     7621 gtcaagcact tctgttagac cctaaaagtg agcgtggcaa ctggaaagaa acgctcccag
     7681 aaatcgccca tgaaatcaat atcgtaaacc taacaagcga caaggacaat gcaggacttc
     7741 ttgacccatt cgtgattatg aagaatgtta aggacgctga aagtctggca atcgacatct
     7801 taacattcct tacgggtatt tcctctaggg acggcgaaaa atttcccgtg ttacgaaaag
     7861 cggtacgttc tgttacccag agcgacagtc ggggcttgct ccatgtgata gacgagttac
     7921 gccgtgaaga tacgccagta tcaagaaata tcgcagacca tatcgacagc ttcacggact
     7981 acgactttgc acacctgctg ttttcagacg gtacggtgga aaatgctatc agtctggata
     8041 atcagctcaa tatcattcaa gtagctgacc ttgtgttgcc agataaggac acaacttttg
     8101 aggaatacac gaccattgaa ttattgtcgg tgtctatgct gattgtgatt agtacctttg
     8161 cccttgattt tatccattct gacagaagta tttttaagat tgtagacctt gacgaagcgt
     8221 gggcgttctt aaatgtggca caaggcgaaa ccttatcaaa caagctggtt cgtgctggac
     8281 gagctatgca ggcaggcgtt tatttcgtta cacaatcttc tggggacgtg gcaaaggaaa
     8341 gtctgaaaaa caatatcggc ttgaagtttg cctttcgttc tactgacatc aacgagataa
     8401 agcagacctt agaatttttc ggcattgaca aggacgacga aaataaccag aaacgcttgc
     8461 gtgatttgga gaacggacaa tgtttattac aggatttata cgggcgtgtc ggtgtggtgc
     8521 agatacaccc cgtttttgaa gaactgctac acgcctttga tacaagaccg cccgtacaga
     8581 gaaatgaggt ggagtgatga aagaaaggat aaaaggtgcg ttcacaaaaa agaagatttt
     8641 ccactttctt aaaatggctc tgttcgtggt ggcactctcc cttatcctgc tttcactttt
     8701 ggggacggtg gctcatgcga cggggcttgt ggacgatacc ataaacgcag aaaatcttta
     8761 ctcaaaatat cccctttcca actaccagct tgatttttat gtggataata gctggtcgtg
     8821 gcttccgtgg aactggctgg acggtatcgg aaaatcggtg cagtacgggc tttactgcat
     8881 taccaacttt gtctggacga taagcctata tttaagcaat gctacgggct atgtggtgca
     8941 ggaagcctac aaacttgatt ttattaacga tatggcagac agtatcggaa aaagcataca
     9001 gacccttgca ggtgttacgg caaacggttt ttccagctct ggcttctatg ttggtttcct
     9061 gcttctcatt atcttagtgg tgggacttta tgttgcatat acgggactta tcaaacggga
     9121 aaccagtaaa gcacttcatg ccgttatcaa ctttgtggtg gtgttcgtgc tgtccgcttc
     9181 gtttattgcc tatgctcccg actacatcaa gaagataaat gagttttcat cagacatcag
     9241 taccgcttca cttgacttgg gaacaaaaat catgctccca aactctgaca gcgagggcaa
     9301 ggacagcgta gacttgatac gggacagtct attttctatt caagtggaac agccgtggtt
     9361 actcttacaa ttcgggaaca gcaacgcaga agaaatcggg gcagaccgtg tagaagctct
     9421 tgtatcggca agcccagagg acgaggacgg aaagaccaga gaggaagtcg tgaaaacaga
     9481 aatcgaggat aacgacaaca acaatctgac gataccgcag gtggtaaacc gcttgggtat
     9541 ggtgttcttc ctattcattt ttaatatagg tatcacaatc tttgtattct tactgatggg
     9601 gataatgatg ttaagccaga tactttttat tatctttgca atgtttttgc ctatcagttt
     9661 tcttctttcc atgataccta gtcaagaaaa tctggcaaag caggcaatcg tgagggtgtt
     9721 taataccata atgacacggg caggaataac gctcattgta acggtggcgt tcagtatttc
     9781 cagtatgttt tataacatct ccaaagatta cccgtttttt atggtggcgt tcttacagat
     9841 agtgtgtttc gcaggtatct ttatcaagtt aggcgactta atgaatatgt tctctttgaa
     9901 cgctggcgac agtcaaaata tgggacgcag gatattccgc agaccgtatc tgtttatgcg
     9961 acatagagct aggcgtatgg aacaccgtat tgcaagggca gtaagtgctg gcggtatttc
    10021 gggtggtgtg gctggtgcgg tggctggaag tgccgttgct ggcaaacgag cggaaagaaa
    10081 aaatacagct tctaaagaaa atcggggcaa taccacttcc agcatgggac agcgagcagg
    10141 ctcaaaggtg ggtgctgtct tagatacgaa aaataaagtg aaagacaagg caaacgctgt
    10201 caaagaaaac atcaaggata tgccgacaca gaccgcttat gcggtatatt ccgcaaagga
    10261 aaaggcaaag tccagcgtat cagatttcaa gcgtggcatg gtgcaggaac agcagtccag
    10321 acagacggga cgattggaaa agcaggagca acgcaggcag aatatcgctg acaagcgtat
    10381 ggagcttcaa aaagcacaag aagcaaggca ggcacagcga aaggctgacg gatcagcgac
    10441 aacgggagct acccgtcccc atgagcgacc agccacagct tcgaccattc caaagccgag
    10501 tgtggaaaaa atgcaggaag tcaaacgccc tgccacagcg accacttcaa agataagtga
    10561 gccagtcaag acaagtgtta tcaaagagcg tccgttatct tctggtgctt ctgatagaaa
    10621 agcgtcccag cctatacagc cagcacatag gcagaatgta gaaaaagtgg tatcgcagga
    10681 aacacaccag aattacaaag cagagcgtac cacaaaggca cagacctttg aacaatcaaa
    10741 gcgtacaacg gaacataccg aaaagaaccg taaccttgta atgaagaaag gacagaagaa
    10801 aaaatgaaac tgaaacatat cgctcttatt ggcagtctgt ttcctatcct cttttctctg
    10861 gtgctgttct ttggtgtcct cattagtgcg gacagcgacg acgaaaacag caacttttct
    10921 tctggcatta cggttatgaa tttatccgca gaagttttga aacatcagcc tatggtggaa
    10981 aaatacgcca gagaaaacgg tatctccgag tatgtcaatg tgctacttgc tattattcaa
    11041 gtagaaagtg gcggtacggc agaagatgtt atgcagagtt cggaaagttt gggactacca
    11101 cccaactctt tagataccga aagttcaatc aagcaggggt gtaaatattt tgcgtccctg
    11161 ctttcttcct gccaaaatca aggtatcgac gacttgaatg tagcgataca gtcctataat
    11221 tatggcggtg gttatgtggg atatgtggca ggaaaaggaa agaaatatac ctttaatctt
    11281 gcagagagct tcgcccgtga gaaatcgggt ggaaagaaag taacctacac caacccgata
    11341 gccgttgcaa agaacggggg ctggcggtat ggctatggaa atatgttcta tgtggaatta
    11401 gtcaatcagt atttaactgt tccgcaggta tcgggagagc tggcacaaaa ggtaatgaat
    11461 gaagcgttga aatatcaagg ctggaagtat gtgtatggcg gtagcaatcc caacacttcc
    11521 tttgactgct cgggactgat gcaatggtgt tatggaaaag ctggtatctc cttaccgaga
    11581 acagcacaag cacaatatga cgctacccaa catcttccgc tttcgcaggc aaaggctggg
    11641 gacttggtat ttttccattc cacctataac gctggttcgt atgtaaccca cgtcggcatt
    11701 tatgtaggaa ataaccagat gtaccatgcg ggcgacccga taggatatgc agacctaagt
    11761 aatagttact ggcaacagca cttaatcggt gcaggacgag taaaacaata gaaaggactt
    11821 gaaaaatatg tttaagaaga ataagaaaca gacagaaact atcaaagaac caaaagaaaa
    11881 aaaggtgcgt actgttaagg taggcacaca taagaaaacc gtgattgcgt tgtgggtggt
    11941 gcttatcgca agcgtgagct ttggagtgta taagaatttt actgctatcg accagcacac
    12001 gacccatgaa aaagaaatca ttgaactttg cttgcaggac accaacggga tagaaaattt
    12061 cgtgaagaat tttgcgaagt cctactacac atggaataac agcaaagaag ctatcgaagc
    12121 aaggacgcag gcaatcagcg gttatctgac aaaggaattg caggacttga atgttgatac
    12181 aatcagaacg gatataccga ccagctccac agctacaaat gtgattgtat ggagtatcga
    12241 acaatcggga acgaacactt tttctgctac ctacgaagta gatcagcaga taaaagaggg
    12301 agaaaagaca agcaatgtga aagcaaccta taccgtaaaa gtccatatag acgctgacgg
    12361 ggatatggta atcgttcaga accctaccct tgcaccagca atcgaaaaat cagactatga
    12421 gcctaagaca ccagaagcag acgcaagtgt agacgctgat actgtaaacg acgctaccgc
    12481 atttctggaa acattcttta agctgtaccc aacagccaca gaaaaagaac ttgcctatta
    12541 tgtatctggt aatgtacttg aacctatcgg cagagattac ctttattctg aactgataaa
    12601 ccctatcttt acaaaggacg gggagaatgt gaaagtcaag gttgctgtga aattccttga
    12661 taatcagaca aaggcaacgc aggtatcgca atatgagctt gtgctacata aggatagcaa
    12721 ctggaagatt ataggataag tattaagggg ttatcaaaaa aagatagccc cctttaccct
    12781 aaaagcagaa aacgttgctc acgtttcaga tatatgttaa tattaaaatt agaatagatt
    12841 aacaaaaaga gatgattggt atggataata gatttaataa atcttggtat ggagataaag
    12901 cacaattagt cgatagtgat aatgattgtg ttattataga ttatggctgt aaaggaaaag
    12961 gtattattac aagttataat ctttatgacg gtatcacact ttgttttctt gatttttata
    13021 cagatgaaat tatgccctca caaaaattta atcctgatat aatttctatt gtccattgtc
    13081 aagcggggcg ttatgaatgt gaattttcta atcatcagat gttttatttg tcagaaggat
    13141 atttcagtat tatggggact aaacatttgc ctatatcttt ttcctttccc ctaaaaaaat
    13201 gttacgcatt tagcttagtc atagataaac acgcccttag tgcagataca caaaaaattc
    13261 taaatgattt ttgcttagat atggacaaaa taagtgaaag attaaagtta aataaaaatg
    13321 gatatttaag tcatgcagac tttgagttag gacatctttt tgaagaattt tataatgcta
    13381 aagataagga aagtattacc tattttcgtg ttaaagcaat cgaattttta tatttcatca
    13441 acaaactgtc gggcaatgaa gaaactgaat ttcgatattt caacaaaaaa tatatacaaa
    13501 taacaaaaga tgtttgcaaa tatatgacag aacatttaga agaaaaagta tctctggaac
    13561 aattagctta tgaaaaagga attaacttgt cgttactgta taaagtcttt gcacaagtat
    13621 atggagatac tccttattct tacataaaaa aatacaagat gaatatagcg tctaatgaat
    13681 taagaaattc aaagcgtaaa ataaatgata ttgcgttaag tcttggatat agtaatgcaa
    13741 gtaaattttc tattgcattt caatcggtat atggtatttt acctaaagat tatcgaaagg
    13801 cagaaaatta acacttttgg gctatttttt ggatagatat atagctatgg agtagaagca
    13861 aataaatata aaaagtaaaa tgatgtatgt tagcaagagc taactacatc atttttttat
    13921 ataacacagg tgtatattcc taacaacaag cggagtgcca ccattcaagc aagcatttct
    13981 aaacgcattt ccatgcttat tagtcacatg aacgattctt tctcacatct tgtagccatc
    14041 tatgctgttg atcacaaagg agaaacatat acagaagaag tgttgacaca gctaatgctt
    14101 aaattaaagt tttcggataa agtaaaagtt ctggatgcaa gtagaacaac tacgaataac
    14161 tatgtatatt ttacagttcg tgagtggctt actaaatgta ttttagaaac ggaacaagat
    14221 attgactgtc tatttaaata ttatgctgct aatatttcgg tcgatttgat ggaagtgttg
    14281 gaggcaattc ctcgttcaac ctaccatgct gccatgcgca cacttttgac atccccaagt
    14341 gatgtaaact ttagtgaagc cgcttcatct ccggatggaa acttcttttt caagtattat
    14401 gaccttataa aaaaactgaa acaaattcag cagagggact actcttgatt ggagggcggg
    14461 caatttgccc gcccttgctt tttgggacaa aatgtcccaa agtggccccg tccatcgaca
    14521 tccttgtacg cctaccccag cggagttaca atataggtag gaggacaacc catcaatttt
    14581 tataggaggt agccgctatg atcgttctca aaatccttgc cgcccctgtg gtggtggccc
    14641 tcactctctt ggccgctgtg gtgtcgttcc tactctgcgt ggcgggagcg gtatgtgtgg
    14701 taggctgcgt ggtgctgacc ctgctggccc ttgttctgtt catcagcggc cagacgctgg
    14761 gtggcgtggt gttccttgtg ctggcgttcc ttgtttcccc ctatggcatc ccggcaatcg
    14821 ctgactggct ggtggcgagg ctccacgccc tcaactacac cctgcgggac ttcatcaccg
    14881 gctgatacct tgggacaact tgtcccaaag ttatctgcgg cggctggcaa cagccgccct
    14941 ttttcgtatg gaaaggaggg ctgcctacgg caacgaccta tttcaagaag cacaagatca
    15001 gcaaggggga aaccatctcc caatctctga aaggccgttt tgactacggc aaaaacccgg
    15061 acaagaccga gggcggcgaa ttgatttcct cctatgcctg cgaccacatg accgccgatg
    15121 cggagttcct gctctccaag gccaagtaca aggccaccac gggccgggag cagcggcggg
    15181 atgccgatgt gctgtgctat cagatccggc agtcgttcaa gcccggagag atcaccccgg
    15241 aggatgcaaa tcgggtgggc tatgaaacgg ccatgcgctg gacgaagggc aagtatgcct
    15301 ttttcgttgc cacccataca gaccgccagc acattcataa tcacatctac tacaactcca
    15361 cttcgctgga ttgcgctcat aagttccggg acttcatcgg atcggcccgg gcggtgcggc
    15421 ggctctctga ccgggtatgt ctggaaaatg acctgtctgt gatcgccaat cccaagctgc
    15481 acagcaaggg ccgattcctc cactatgggg catgggcagg tgcggatcgc cagccctcct
    15541 acaaggagcg gctgcgccat gctatccggg atgccctcgc caaaggcccc gcagacttca
    15601 acgccttttt gcggctcatg gaggaggcgg gcttcacggt caagcagggg cgtggcggtg
    15661 cgatctcatt cctcgttccg ggtcaacagc gggccacccg gctccgttcg tccaccctcg
    15721 gggaaggcta cgatcccgag gacatccggg ccgtgatcgc cggaaagcgg cccttacccg
    15781 aggtgggtgt tccggctccg gcggctcccg cccgtgtcaa tctcattgtg gatatacagg
    15841 agcggctgcg tgaccgcaaa ggccccgcct atgagcgctg ggccaagatt tataatctca
    15901 aacagatggc cgccgctctc caattcatgc aggagcatgg gctgaccgag tacaatcaac
    15961 ttgccgccca aacggaggag gccgttcagc gtttccatgt gctgaccggg cagctccggc
    16021 agaccgaggc caacctcgcc catacctccg aggtgatggg ggccgtggtg cagtatgcca
    16081 agacccggcc cgtgttcgat ggctacaagg caaccaagta cagtaaaaaa tatctggccg
    16141 cacacgaggc ggagctggcc gactaccggg cggccaaggc cattttgaat gatctgctgg
    16201 atggggcaag gctgcccagg atgcccgagc taaaggaaaa gcgccgcact ctggcggcgc
    16261 agaaaaagac actatatgcg gactaccggg cggcgcagca gcgtatgcgg gaggccgtgg
    16321 cggtcaaggc caatatcgac catctgctgg ggctgactga ccagcgcaaa aataaggaac
    16381 aggagcgata gctacggtga cgcagacgat caaccttggg acattttgtc ccaaggtgcc
    16441 gggtttgggg aggctcccca acaagcgttt ttgcggctcc ctcggggcgc aaaaatagca
    16501 agtgtggcta cacttgccct gcttgccgtt ccgtgcaacc ccgaaaatgg cgcaaaaaaa
    16561 cggagtctgc gttttttacg cttcctccgt tctccgggcc cttttcagtc cctccgctgt
    16621 ggcttcaata atggttagct ctttttcgtc catttcgtta agcaagtgat ctatatgctt
    16681 tcggcaatca ctttggccgt tctgcttgtc tggatagaaa aaatcatcta cggaaatatc
    16741 caacagggtg acgagctgat aaaaagtatt gaggctgggg tgttgtcctc tgttttcaaa
    16801 atacatgata gagcgcgggg tgcggtctac aagctgtgcg aggtattcct gtgtccagcc
    16861 tttggcttct cttttgcgct tgatttcccg gcccagcgcg tgaaaatcaa gttttcgttc
    16921 atcttggtac attttcatct cacccctata ttattttaca tttcgggtga tattatttga
    16981 acgaagtgta attttatgtt tatgtagtat tttgtttcgc catatcaaaa tgataaaatt
    17041 atgtattttg aaagacagct acgaacaaat attgaaagac tatactaagt cgttcctctt
    17101 gacaaacatc cgtattcgga ctacaatata attgtccgag tacggatgtt tggagggatt
    17161 aagatggcag agataaaaga acagctatct caactcaata aaatgtccaa taaaagtgaa
    17221 gctctttatc acaaagttgc tttttctatt ggtatctctg atagctcact tggggtactg
    17281 tatacgctgc tggacgcaga ggcgccgtgc tctcagtatg acctttgcag cgagtggtct
    17341 attcccaagt aaactatcaa caccgccgta gctgccctgc aaaagaaagg gatggtgttt
    17401 ctttcgccca tccccggaac aaggaataag aaaaatatag cgttcacaga gtttggtaga
    17461 gattttgccg aaaagacggt tggcatattg cgcatggcag agcttgacgc tcttgcggaa
    17521 ttatcaccgg aagaaagaga gctttatatt cgtttgaatg aaaaatataa ttgtcgcttg
    17581 atcgagaaac tttatcgcat tatggacgag gtgaaccaag acaggaaaga ttgcgattaa
    17641 ataagaagga gaaaatataa ttatgtccaa gtataaaaaa atgaaacagc tcatagcgga
    17701 tatgcgctta cctgagtatc gttataagca actgcttgat gctgtgttcc tacaaggaat
    17761 tatgcgtttt gaagatatga agttgcttcc aaaaacgctg cgcgaaaagt tggtggagca
    17821 gtttggtgaa actgttgtgg agatcaaggc gatccatcat gagaaatcaa tgcaaactga
    17881 caaggtgctg tttgaactgt cggacggaaa ccgtgtggaa acggtgggtc tgttttataa
    17941 agagggctgg aactcctttt gcatttcatc tcaaagcggt tgcggcttcg gttgtaagtt
    18001 ttgcgcaaca gggactttgg gattacgcag aaacttgact gtggatgaaa ttaccgacca
    18061 aaacctatat tttatgcaac aaggatacag tatcaacagt atctctttta tgggaatggg
    18121 agagccgttc gctaatccac aagtttttga agccctgcat gatctgaccg ccccggaact
    18181 gttcgggctg tcccagcgac gcatcaccat ttcaacttgc tcatcatttt ccaaacttat
    18241 attaagatct tcatcaatga taggtagctt aaaagtaatg gattttagcc attgtccatt
    18301 aggctgcttt tcttcataaa tttgaatttc tgaaatgagt gcttctatta actgcctgcg
    18361 ttcaacatcg ttcatgactt tataaagctt gtcaaaataa atcaatactt tatatatgtt
    18421 atcaccagta agtttctcaa cctctatcgt ttcttttttt gctttagctt ctattaactg
    18481 actttctaat tcctcaattt tatcgtacat acgataaagc ctatcatcta aatcattttt
    18541 tcttctctta taatgtttat cctctacatc taaattatct atttcctcaa taagcttata
    18601 ctttgtagaa tgactttttc ttagttcatt ttggtaatta tctatctcat tttcaatagc
    18661 agatgtatca accttcatgt taattttttc ttgtatcata gatgcaaatt tagggttgct
    18721 tactaacttg attattacct cagcaacggc atcatctaac aattcttctc taatttgttt
    18781 attataggta catttatgtc ctctattcat aagtctatgc ttacaaccat agtaatagaa
    18841 gtctttatac ttcgtcccat cttttttata ttttatagat ttattcccaa acattccagc
    18901 tccacatatc ggacacttta ctattcctga taataagtgt gtcctcatgt tcttgccctt
    18961 atttacatgt tcatactttt tagcttgtgc tttcagttta acttgtgcag cctgccacaa
    19021 ttcatctggg attattgctt catgaatgcc ttctgctact aaataatcgt cctgctctac
    19081 ttgtctgtat tcattccttg taccatgaac tttttctaaa gttcttcttc caaatgctat
    19141 tttcccgtta tacacaggat tctttaatat ttttcttata agacctgcgt caaacaatgg
    19201 atttttccca ttttgtcttg gtattttcct tataccatga ttttctaaat atttagaaag
    19261 accattagct ccaatacttg tatttacata ttgatcaaaa atagttctta ttgcaattgc
    19321 ttcttcctca tttattatca atttcccatc aataagttga taaccatacg gagcaaatcc
    19381 accattccat ttaccctctt ttgccttttg aatgcgacct tccattgttt ggacacgaat
    19441 gttttctctt tctatttcag ccactgccga taaaacagaa atcatcaatt tacccgcatc
    19501 tttagatgaa tcaattccat cttcaacgca aataagattt actccaaaat cctgcattat
    19561 ttgtaaagtc gaaagtacat cagcagcatt tcttgcaaat ctcgatagtt taaatacaag
    19621 aacgaaagac actccatcct ttccggattt tatatcttcc atcatacgag taaactgaac
    19681 tctaccttct atagattttc cagacttacc tgcatcttcg tattcaccaa caatctcata
    19741 atcattatag agagcaaaag ccttcattct tgatttttgt gcctctaaag aataaccatc
    19801 tatctgtatg gatgtagata ctcttgtata gagatatact tttatttttt cttttgacat
    19861 agcattaacc tcaatatcct tattatatat ttatatattt ttaccaataa aggtttggac
    19921 tttaatctca agtatattat agcactttta tttctctttc tcaatccgtg cttttagcta
    19981 tatcaaattt attcttatcc tctgtatttt ttactggagt aggatttgga agattctcta
    20041 aatctaaaac tccagcatat tttgtaatca gatttgctat caaatcagcc aatccattcc
    20101 agtcattatc cactaataca cctcctccac tttaaattct ttataaccaa atattatttt
    20161 tcttactaag ttttatgaca ttggtaggtg cattggctgc tacataggaa tctcacctcc
    20221 gccccttgtt tcagacgagg cagcttaaac tattgaagta tcattatcac cacttacatc
    20281 atcgaaataa gctgccacgc ttatctttta tgtattccta tctatcgctc attttcttct
    20341 aacctcggca ttatgccggt attcatttga accgattttc atcagcagaa aagtcttggc
    20401 gtaggtcata actctcagta agtagataaa gtcctacctt ggtctattca gttgtcaaag
    20461 atcaatattt gaaagggact gttttcttta cattttccta tttgccataa gcaggcgtat
    20521 ctcctactca agaaagtaaa ggaggtcaaa ctccccttca ctttacataa ggaaaatttg
    20581 ccccctttgg ttaccaaagt tttaaagttc ttcgataaat tttttcagct tttcaagaat
    20641 tttgtttctt cttttgatta aagccggatg tgtaatactt ttgagctttg ctactgaacg
    20701 aacagtttca tcattgaaat acaagcgttc aataatatct ctttcttctg cattgagcct
    20761 tgttatagca cttcttactg cttcaatcat catctgtgtt tcaacaatct tttctacatc
    20821 aacactttca tcaacaatat tatctgtaaa gtgtccgtca tgatccaatg ctgaaaaaaa
    20881 gagcaagtgg tttttcctgt ccacctgctc taaatacttc tcgtgttctt tttctcgcca
    20941 gtagactata tatatatctt cactgacttt taccttttgt cctctgatat aaagataata
    21001 ctcttttgcc ataaagtttc ctccatctct tttttatctg cttgtttttc agataaaaaa
    21061 ggaggacttc tgcatcttgg cataagaagt cctctgaaat gaaagaataa aaatatttct
    21121 ttatatgaac ttgattgttt caattcgcac ctgaaacaat catctactat acatattata
    21181 aaaaagagga atgtattgaa ttctaaatat ttctaacatt aaacgtttag atgcttccat
    21241 atttatttcc tccattttat caataatcta aataaccatc atattcatct actacttata
    21301 taattgagtt ttattaaact ttttaagttt ctattctatc aaatatttaa acagtctctg
    21361 catccgaaaa gactaactca tatagtttta ctaacaatat tttcattagc gtgttcaaat
    21421 cctgatttct tgtatcaatt attttctgtt cactttcaat aagaaaaaag cctttacttc
    21481 catggctaat aataagtctc tatttttcaa taactattgc aaaattatat cttgtttaag
    21541 aaaatatttt aattttaaag ataaaaaatt cttcatcctg caatgcttta tattctatat
    21601 tgtaattatt cagaatatta cttacaatat ataaacctaa tccattacta ttttccttat
    21661 ttaaatcaaa tttaacatca aatattttat ccatattcga aattttattg ttaccgtatg
    21721 aattctctat atataaccaa tcattaacta tcccaatatt aattacccca tttacatcag
    21781 tatattttac cgcattacta atcaaattag atagaataat ctttaaagct gtttttccta
    21841 tataaacatt ctcatctaat atataattat tgacagttat tttcttttga tttgctaata
    21901 tttcatactt ttttaatatg tcttctaatg tatcatttat tttcaaatat tcttcatcat
    21961 tattcaaatt ttctatagaa tgaactgata atatttgaga aatatttttt gttaaactat
    22021 caacgatttc aatacattca ttaatataaa tatctctttc tttatatttt ccaatattgt
    22081 atttcatgtt ttccagtatt attttaagac tagcaagagg agttttaagt tcatgggatg
    22141 cacctttaaa aaagtcatat tttaactttt ctaattttaa aatttcttta tttttaaatt
    22201 ccaaatcgtc aattgttctt aataaagtag aatacaaatc attaatttgt tgttttaatt
    22261 ctccaacttc atcattagag ctaactttta aacgcgtttt tttatcaagt ttcatcattt
    22321 tatcagtaac aatttttatt tcttgtatat tattttttat tgattttgca taaattaaag
    22381 aaataatagc agaaaataaa attgatatta ataaagaata tggtaaaaat ttaaggctta
    22441 aatctttcgc atctttttgc atatcagctg tagaaacaaa ttgtagattt gtttttttac
    22501 catcattaag ctttatttct ctttcttcaa ttatcaaaga attactatca ctttctaaat
    22561 taacatttat attatctttg atttgtattt cattcttatt atttttttct tttataaatg
    22621 cttttatttc actactctta gaataaagct ccaaagtttg ttctatatat tttatttctt
    22681 ttccattcat attactagaa atttcatttg ccatattatg aatcttttct tttctcgtct
    22741 ccaaatatgt tttaggaaag ataaaaaaaa ctaatgaatg aactaatata attattattc
    22801 caagaacaga aaatatttgt atgaacattt ttggaaatat ctttaatttt ttcattttct
    22861 ctccaattta tatcctacat ttcttatagt agttatacag tctaattgta atttttttct
    22921 aagttctttt atatatacat ctattactcg atcataagga gtttcttcgc tatctttcca
    22981 tacataatca ataatttgca ttcttgttaa cacttgtcca ttattatcca ataaacattt
    23041 taatacttct agttcttttg catttacatc tatttcttca ccatttattt tagctgtata
    23101 gctattaaaa ttaaccgata gatcattata ttcaaacttc tctaaatgtc cataattctt
    23161 cttaattaaa gaatctattc tagcctttaa gaccggcaat gaaaatggct tttctacata
    23221 tccgtctact aaattagtaa atgcatcaat tttatattct tcatcactaa atgcagtcaa
    23281 gattagaatt ggtaaattgc ttttctttct aatttctttc aacacttcta aaccatttat
    23341 aaaaggtatc tgaatgtcta agataaccaa atttatatcg ctattaaatt ttgacaaggc
    23401 ttctcttcca tctttagctt gaataacagt atatccaaat tctgaaagat attcacttat
    23461 tccctctctt atcatattat catcttcaac agttaatatt ttcatatata cctccactta
    23521 agcctctaca cttatattat acagcatatt atttgatatt taaaagccaa aaaatttttg
    23581 gtgctaaaaa gcaccgaaaa ttttaattat tgtcaattaa taattctttt ggattccttc
    23641 ttaatatatt gaatgaagaa ataatcaatg atacaagaag tactaaactc ataaatataa
    23701 ctacataaat tatgaattta ggtaatacat ttatatctaa acttgataat gtcttgttaa
    23761 atccttcagc ttctgcccca ccacctaatt ggctagatgc agattgtcta gctatttgtt
    23821 tagctatatc accagtaacc ttattcaata tattattccc aagcttatca gctgtatatt
    23881 gagctaagaa ataagaacca acgaatgcgg ggattgatat aaataccatc tcaattagga
    23941 attgaccaaa aatttctaat tttgatatac ctaatgatag taatactgct atttctttct
    24001 ttctagcatt catccataag aataataata atgaaactac aactcctgca aatattaatg
    24061 agccaacaaa taatttattt gaaattgagt agatacctga tatagattgt tgtaatgcag
    24121 ggtaatttga tgaactttta atcaaattat attctctcca atttatatct aatttcccaa
    24181 gatcttttat tacactatca agattcttat caccttttac aaagaatgtt gcatcttggt
    24241 atacagctgt atcttctgta ttaccataaa ctttagcagc tgtatgaaca tcagtgatta
    24301 atgtattttc gtatagttct tgtgctgcac ttactccacc gctattatgc ccatcgaata
    24361 gacctttaat ttctacttct actgtttcat ctgcaccttt ctcattatca gcatcaaata
    24421 aattagattt tatttttatc ttatctccaa ctttaagatt atttttttca gctaaatctt
    24481 tatgcattaa gattttattt ttatcttcat tttctaaatg ctttccttct actaatttat
    24541 atgcttctga tacaaatttt gtttcttttg atgagtcgtt aaccccagtt aacataactg
    24601 ttcttttaaa attcttcgct ctttcaggtg attgattcgc aagagtctct tgagtttcaa
    24661 taatatcata atcaactaaa tctgcaacac tgtttattct ttttacatag gagtctatac
    24721 tatctgtttg aaatatcttt ttaatatctt caccttttac atttccccca cctctaggtg
    24781 ttcctggatt tacttgtcta tttatctcca tagaaaaact atttgttata ttagcaaatg
    24841 tttcttttga agctctgtcc gtagcatctt taatagataa actaatgaga cttaaagttg
    24901 acatagataa aataactaat ataataatta atgattttaa gctttttcta gttacataag
    24961 caaatgcatt ttttatcatt attcaacctc caaattcatt ttatttactt ttttcaactt
    25021 tttaccactt agttctaaaa tgatatccgc agaatctgct acttccttac tatgtgttac
    25081 aactattaca catttatttc tatctttagc taatgtcttt aatatattaa ttatttctcc
    25141 agcagtaaca ctgtctagat taccagtagg ctcatcagct agtattattg gagcatctga
    25201 taccaatgct ctagcaatag ctactctttg ttgctgtcca ccagataatt tcataacatt
    25261 tctttttatt tgttttttat ctaaacctag ttcaaacaag atactttcat ctgctgattt
    25321 atttactaat ctaatatttt caattggtga taaataatct attaaattat agttttgaaa
    25381 tactaaagat atattatttt ttctatgatt actatatcct ttcttttgta tatcttcatt
    25441 cttaaacaat atttttcctg tttgaggttt atcaagtcca gcaagtaagg aaagaagtgt
    25501 agattttcct gttcctgact ttcctactat cgcataaaac ttcccaagtt caaatttttg
    25561 atttactcct gacaaaactt tttctttaga atttgcataa ctatatgcta cattctttat
    25621 ttctaatata tccattattt tctcctaact tatttttgat aatatttctt taggcttctt
    25681 gattaatatt aatgaagaag caaatacaac tgataaaaca ataatactta ttaatattaa
    25741 ataactttgt ccaagtgttg ttatatttaa cataaaacta ctattattta ttaaacttcc
    25801 accggaaatc attgagtcct ctgagttaat gaatccatct acaattactt ttagtaatac
    25861 atttcctaaa aataaggaag atattatact tggtatcgat atgaatatta actcgaatat
    25921 aaattgcatt ataatttgta tcttagatgt tccaatagat aaaaatatac ctatttcata
    25981 aattctttct cttaaccata gaatcaagat taatgaaaga acaaccatcc cacctaacat
    26041 aatagaataa gtcattattt ttattatatg ttttattcca ctcactgact ctaaagactc
    26101 ttcaaacgca ttagaatctt tttcaacaaa atactttgac tcatcaattt taagctcttt
    26161 caatttgttt aaggctaagt ctgtagattc tgcactacca gaatacatta aaattttatt
    26221 tgcgatttta ttattttctg atttatttaa tatctcttgg cttgttgaat aatctacaaa
    26281 caccatattt tcactaaaat cagaagataa tcctgtatat gtttcctgtt ttttaccaga
    26341 aaagatccct ataattttaa atttatgact ttttattttt ccactttttt caatatctag
    26401 taattcaaga tcaacttcat cacctaattt tagattgttt tgtttagcaa attcttcatg
    26461 aacaataatt gaattcttat cattttctcc tatatttttg ccttctttaa ttgtaaatac
    26521 tccactacta aataaaatat ttcttttagt attatttgta gcttcgagtg aaacaacatt
    26581 cttaaattcg tcagataaat cttctctatt tattctttgt tctccgctaa ctactttagc
    26641 atcttttagt tttgctaatc catcatattg aattattttt tcttcaattt cttttaattt
    26701 ttcaatatct ttaaattgat taacattaaa atatttacca tctttttttg ttattgatat
    26761 tgaagaatta gaactttcat ataaagcctt ttctatttca ttgcttgatt tcattattgt
    26821 taaacaagaa tacaagcaag aaagaactat agttaatatg ataaaaatta ttaaagttct
    26881 atttctcttt cttgtaatat atgctatagc attttttatc actttcctaa ctactcctct
    26941 ctattttctt actccataac taattgcatc cactggacaa gcatttttac atcgtccaca
    27001 tctgatacac tccagatgat tgcaattctc aactggatct atattcattt ggcaaacttt
    27061 tttacatttt ccacaattaa tacacttatc cttatccaat ctatatctaa atattgaaat
    27121 agggttaaaa attgagtaaa tagctccaag tggacatata tatttacaaa aaggtctata
    27181 tataattata gaaagtaaca aagtaactat tagaatgatt cctttccaat aatacaagaa
    27241 acctatcgca tttctcatac ttttatttaa taaaacaagt ggtaatcctc cttccagcat
    27301 accaatcgga caaataagtt tacaaaaata cggagaaccc tgacctaaaa tatcaaccaa
    27361 aaataaaggt aatattatta caaatacaag taatattatg tatttcaatt ttctcaaatg
    27421 tttatcaaac ttaaatgttt caactttttt aaaaaaggga atcttatgta gcaaatcttg
    27481 aatcaatcca aatggacaca gccatccaca aacaaatctt cccaccaatg ctcctataaa
    27541 aaacaaaatc ccaactatat aataactaat cttatatttt gaatttccaa ttactgcctg
    27601 tagtgatcct attggacaag aaccttttgc tcccgggcat gaataacaat tcatacctgg
    27661 aacacaaatt ttctttaaat tcccaccgta gattttacca gtcttaaacc cttcaaagta
    27721 agaattagta attaaaaacc aaacggattg aaataaatgc ctaaaacgat ctcttatttt
    27781 atatttatct ttttttgtac taccatttat aatattttta attctatcca attccaatac
    27841 actccataca aattcttatc gccttatcca agacaacttt agtttctcca ttactatgac
    27901 catagtaaat catcgccata ctaataaaga taattaataa agacaaggca cggcttttta
    27961 aaaacttatt aaccatatac caatcaccgc tttactttta tttatttttt attttttcca
    28021 attctttttc aattacctgc ttccattcct ttgaactctt tgccccagaa taagtatcac
    28081 ctacaatatt tccattacta tcaacaaaga aagtctctgg catagcctgt ataccattca
    28141 atctaccatt aaagtttgtc ttatctggca ttaagaaagg atatgaagcc tttgtctttt
    28201 catgtattaa ctttgacttt tcaattgctt ccttgttttc accgttatca tcaacagtat
    28261 ctgtaacaac tccaacgata tttacaccct tgcttttcat ttcattctga acctcaacca
    28321 aatcaggaat ttcctttaca caagctgtac accaagttgc aaatacattt accatagtaa
    28381 gatcatactt ctcaaaatct ttacttgtaa aatccttgcc atttatgtcc tttgttgata
    28441 gtttacgtaa atctttaacc gattctttgt taaatgcttc tgtattttct aaatcagttt
    28501 tttctgatag cacaaaacca tttttaggac gttctttttt atctataatt tgaatctcag
    28561 ttctttttaa ttcatccaga agattacttt cagctccgct atttgtgctt aaatagcaat
    28621 cgtattttcc atcacttgaa actcctattt tagtatgagt atcacatttt gtaattttag
    28681 atattttttc ttctgatgta tttttttcga atataccaat agtcccaatt ctttcaagct
    28741 cattttgcca atttttatat ccatcgccca tcttttctat aactgcattc ttttgttctt
    28801 ctgtcatttt ttcaaatgtc aaaatagcat actttagttc tttatctata ggactttgat
    28861 catctaacat cgctatcttt ttatcatcca tatacttttt aaatttgtct gaaagtttaa
    28921 atttaagacc taaatattca taaacatatt ctttttttgt ttttaaagta taatctgatg
    28981 gcttaaatgt ttcattttta tccttctcat tagttgacat acctgcctct tcagaactta
    29041 tagattgtcc ttctttaatt ttattgcttt cattactttt tttacaccca actgtactta
    29101 agctcaaaca taacgtaagt aataggcatg ctcctcttga tactattttt ttcataaatt
    29161 tttcctcctt tattcctcct ttatttttac acaatagcag tttataacta ctttcaataa
    29221 tattctacac accttttatg aaacgttgat gaaatataat tttttatttt tccaaactat
    29281 ggtataataa tataaatatt atgcttaaag ttgttcttac acgattagta tatgatcgat
    29341 tagtatatga gcaagcacag taagtgtacg gacacttacg aaaaaattga tgaagcagcc
    29401 ctctgggata tgcttctttt tttatcaatt tttaacttgt tagggtatac cctaagaccc
    29461 cgaaatacat tcaaaggagg atttacctat ggcaaataga atacgaaatg aaagactgga
    29521 aattaaacta actgaagaag aaaaggctct ttttgaggag aaacgaaagc tcgcaaggtg
    29581 cagaaatatg agccacttta tccgcaaatg cgttttggaa aaggaaatat atcaagtgga
    29641 tttagaaccg tttagagatt tacagggctt actgtccaat gctacaagca acatcaatca
    29701 gattgcaaag cgtgtaaatt ccaccggcat aatctataag gacgatataa acgatatgaa
    29761 aaagcagatt gaatatttct caaaagagtt gtggcaaata cattctctgc ttcttaacag
    29821 aacttctgga ggtgattaaa tcttatggct ataacaaaaa tacatcctat aaaatcaacc
    29881 cttaatcttg ccatttcata tattgttaat ggaaaaaaaa cagatgagca aatcttagta
    29941 agcactcata aatgccatca agaaactgct catacccagt ttttaagaac acgaaatgat
    30001 gttggaacaa acggaactgt tcttgccaga caccttattc aatccttttt acccggagaa
    30061 gcaagtccta aaatggcaca tcaaatcggt cttgaactgt gtaaaaagat attaaaggac
    30121 gagtacgaat ttgtcttatc tacccacata gataaagggc atatccacaa ccatatcatc
    30181 ttcaataatg tgaatatggt aacaggcaag tgctatcaat ccaacaagaa aagctaccac
    30241 aaaatcaggt atcaaagcga taagctatgc aaagaaaaca acctttctgt gattgatgag
    30301 ttttacgaaa gctataagag aaagtacaaa acaaatggca agtcatggta tgaaaatgag
    30361 caatctaaga gaggtacttc ttggaaaagc agacttcaat ttgacattga tcgtcttata
    30421 aaacagtcta agtattggga agaatttcta aaaaaaatgg cggagcttgg ttatgaaata
    30481 aagtatggga aacacatagc ctttaaacca aaagataaga caagatttac aagagctaaa
    30541 acgattggtg aggactatac agaagaaaga ttaaaagagc gtattacaga aaatcaatct
    30601 attacaactc cacctgtcaa aagacgaatc ggaaatatca tcaatatgaa tgccaatgcc
    30661 aaagtgaaag agagcaaagg ctatgaatat tgggcaacca aacataactt aaatacaatg
    30721 gctgagtcag ttatttttat cagagaacat ggcattaact ccattaagca gcttgataaa
    30781 tacattcaaa aaagtgctga agaaagacaa gacttacaag ataaaatgaa agaaattgac
    30841 aaggatatgc agctactttc cgatacaatg gagcaggttc atactgttaa aaaatatcga
    30901 gcctactaca aagaatataa agcaaaccct tccgataaag cattttttga ggagcataag
    30961 gctgaaatta cacactatga aatagctctt tcaaagctaa aaaaatccta ttcaaagctg
    31021 ccgaattcaa aggatatttt agataaactt aataaactgc aagaaaaaaa gaataccctt
    31081 attcaagagt attcttctac aaaatctact atggacgaac tttatcaaat acgaaagaac
    31141 tatggaattt acatgggtaa ggagatggaa cgataactct aatattatat agctttctta
    31201 tttttataat attaaattga aatgcctatc aatgatgcaa gcatttttat acctaaagct
    31261 aaatcaggaa ttaaagctac ggaagtagtg ataaaatgca tactttttaa acaagtagtc
    31321 aatacttctt tcttcggatg cttctgctta atttcatttt ttatattatc taaattctga
    31381 ttaagtattg atttggagtt atcatcctcc atacctttaa ttaagtcttc tacattgtta
    31441 attgccttga ttacttcttc agtattaaat ttattttctt ggtcagcgtt aatcgtgctt
    31501 ccgtgttccg caatattgac ttgaactcca gaactattaa cattgatatt atatagagca
    31561 ttattttcat cgtaccccat atcggtagaa atatctttca tataattttc aacctcaagt
    31621 acaaacggat agaccattct atcgccaaac gcctttgcca tatcttgata tttattactg
    31681 ccagcataat accagccaaa agtatatgta ggtagattat ttcctgttat aaaggaaaat
    31741 gctctataca gtagatacgt ccttttatta ctatcattgc caagattcaa aactttacgt
    31801 ccataagaat tacttatcgt atttaataag tcatctaagc catcaacatc atattctaaa
    31861 ctatgcaaat actcttttat caacaatgtt tgctctatat aatttatcat ttcttccaaa
    31921 tttgcattct gttctccata atatgaattc ataacatttg aagctaatat cctaaatttt
    31981 ctactgattt ttctaagttc ttttttacta atctccatct atcatctctc cacaaaacgg
    32041 gcaataacta ccttctaatt tttttatact acttattcta gcggttttgc cacaacacaa
    32101 atatttcttt tcttcataat ctccagtttt tcttcctatc ggaagttctg cttccttttt
    32161 aatctctttt ccacgtttaa actttatact tttactattc ctaaaacttt tttcaagatt
    32221 ctttgaaaaa tcatttagca tttcagcaac ttgattttca actattcgtt ctgctaattc
    32281 ataaatatca tcgtctaaat aattctcgtg tgttaaacca caattaggac accaaatatc
    32341 tattgtgctt tcagcctcaa tatcttccac cgttaacata aatttttcac tacaacttgg
    32401 acattttaat tgaacatatc cctcttcatc cgctggaaca gaaatttcaa attttatatc
    32461 attagccatt taaatttcat ctcctcacaa taaaagttat aatttcacca ctggtagact
    32521 acaaggtcgt gcatctttac gtacaataac aacactattc tcagcacgtg taacagcaac
    32581 atataatcta gccttacttt gttcttttag agatgtgtta ttgttaataa tccaatccca
    32641 catcggttga gtcggatata taagtactcg ttggaattcc aagccttttg attctccaaa
    32701 attataatat ggatattcat tattgacgga tgtcctttta ttttctctca actgcattgc
    32761 attaaatttc tgcaaatact tctctacatc tgctttatca attatcataa caccatctga
    32821 ttcatcttca gctctacgca caaaatttgt agatgttctc tctggataca gactattcgc
    32881 aaatttacat ataatatcat tgtttctata agtacaatta agtgtcgttt catcaattct
    32941 tacatctaat tttctgcact cattttttaa aaactcatct attttcccac aactatattt
    33001 tttatatttt tttgatgagt gagttgaata tgtgcattgt ctaggatccc cgaccattat
    33061 cacttcaatt cttgaagcta ttaaacactt taatatttcc aaatcatacc ccaccatatc
    33121 ttggacttca tcaacatata tacagtcata cattccactg attctttcaa aaacttttcc
    33181 attacattta tcatttaact ctacaacaca ctctgataat ttatcagtat ataccctgtt
    33241 atctttggca aaatatttgt tggatgtttt ctttacgtac gtattgttct gtccatttac
    33301 aaatataatt ccttcaattc gttccttaaa tctacttcct tgataaggtc taataccatc
    33361 ttttaacaaa aaagaaaacc atgtagaaac tgtaatatta ttaggaatta caccaacttt
    33421 attcttaatt cggctaatta tttgttgctc atttgtaata gtatatgtta ggattagtat
    33481 ttttttatga gtattaagag aatcctctat aatttttctt gtttttccag atccagcagc
    33541 agctatatat aatttcccac tcatattaga ataccctcct ttatatattg tggaaattct
    33601 atagctttat cactttcaaa aatctttaaa gcaacttctg ttttatgccc cttcatatat
    33661 ttaagtaaat catctggatc ttcatatgat ttattcaata tctcgtttaa aatagacatg
    33721 gaattcgatc tatatataca cggttccaaa gtattatagt tgtaattttc caaagaacca
    33781 ttatattcat gggtactatc atcaaaatat atatttatat tctcacaaca attatctcct
    33841 aaataatcgc tatattttct atttagtgca tcaatatttc catcattatc ggttatcact
    33901 gacacacgta attctagcaa ttttgcgatt tccaaaaatc ttaaaaaaga aattccaacc
    33961 gatattatat ctattccatc attaataggc aatttaccat tatgagagtc catatatgct
    34021 ctttgaacaa ttagttcgtc cgaatcacct tcgactaaaa ttgctttatt tgataataat
    34081 aatcgtaacg tatcataacc tgcaattctt ttaaaaaata aatttgtttc atttgataaa
    34141 tcacctagcc gtagtgtttt tcctcgattt agtaaaatta aattttcaag tcctaattta
    34201 tttgatacaa aactactatg cgtagtaact attatctgtt tttcttttac tgactcattt
    34261 actttatcta ggagaatatt taagtttgaa tatgacaaat ggcattcagg ttcttctaaa
    34321 agcacaatct gcttattttc tattttatga tcacttaatg ccaattgtgt tttaacaata
    34381 cattgtgtac ctttaccaat gttattatat ggtatttcat ttaattgggt taccaagctg
    34441 ctttcccatg aagttttcgt accgaaatct acagaaattg tcaattcgtt atttgatgtt
    34501 tccgttaatt ttctatttat attagcaata ttctcatctc tagaaaattg ttctttcata
    34561 agcctatgcg cttgcgatat attaataatt tcattatcat ctaacagatt ctttactatt
    34621 ctcgatatgt atatatctga cccactggta ggagtatagc ttgtcgtatc aatcattgca
    34681 gacttaattg gaatgctcct tgcagttata ttttctctgg caaaagacat ccaacttacc
    34741 tcataatatt caattggcaa cgatgttatc ttgttcttac aaagcatttt atactcattg
    34801 ctatattttt catcaaaact aattgataaa caaatcccag gggtgtcttt agttttttca
    34861 gaatttccat cacctaaaaa acttggcatt tctgcatcaa aatacgcttc aataattatt
    34921 ttaggaggtt ctacattcac tccttcgttg acttctctaa tatagttttc cactatattc
    34981 ttattgaaaa ggtactgagt aagttcattc ctaatatttt tcccatgata agttccagtt
    35041 aaaaccagat taatagcttc caaaattgta gatttacctg ttccattatt gccaactaat
    35101 atattaatcc cattttcaaa atcaactata aaccaatctg aaaagcattt aaaattcttt
    35161 atttttaatt tcctaattga cattttatcc cccttcttta tctaatataa aaatcatgat
    35221 tttagaatat aaatattgat aactatataa atacagttat gagtttcctg tttaatcaca
    35281 atcataaatt ttttatgcta catcccttaa tattttttca agcaataatt taacaataag
    35341 ttttatgatc ctctagctcc tgaaaataaa tctatgactg taaacttaga ctcccattct
    35401 ccttacatct tcaataatta cgcactccat aatatcttca atatcacaat ccaaagcatt
    35461 acaaattcta agcaaaacat ccgtagttac attctgtcca ttcttcattt tgtaaaatgt
    35521 acttttactc acttttgcct tttccatcaa ttcattattt gtcatatcta aatcaatgag
    35581 ttttttgaac aatcttttat aactaaaaac cctcattctt atcctccgtt caagaaaact
    35641 atatctagta tattatacca caaaatatac ttttttcata aatatattct ctttaatcga
    35701 acttgcataa ataaaaaaga gccggtgcag tcctaagacc acaccgaccg taaatttatc
    35761 tctcagcttc tttgcttgct tctttctttt ctttcggttt ttccttatct tcagcctgat
    35821 attttttgat agctccaagt atagattctt tcttttcttc ctgtcctttc atctgttcct
    35881 ttgcctttaa cagttttgaa gaaagtatcc tgatattgct atgctccttg ccgttatcat
    35941 caatggaagt tctgatctgc ccaaagagtt tgacaaaatc tccctgctta aagtcctttg
    36001 gaatatcact cttttctcca taaacggagc agttgtgata taacttattt ccctcatcat
    36061 ctttagatac tacggagaag tttgctacct taaaggtttc tccgttctta ttttcccttt
    36121 caattacatc aacttctcca acgatatttc cgacaatatt gataagatta tccttttctt
    36181 cccgaacata aagcaggtct tttatctgcc cctgtcccct taaatcctca atcatatagt
    36241 caaactcctc atgcagcaaa ttaaggctat cattgtccat ataagcgtca tatagcttat
    36301 ctaaagcact ttcatcattg ataccctttt ccatgcttat aatcgcttta acaaagtctt
    36361 tatgattatc acttaacata tcttcaactc tatctctcat tgttttgtaa tccatgtttt
    36421 tatctccttt catggtaaaa gggaggttgc cctcccttta tcttaaaaat tcctgctctt
    36481 ttattttttg tttttcttct gttttacttt cttcctgata ggctcttatc tgtcctaaga
    36541 tagacggctt atcatctgat ttttgtgtgg tttcttcttt gctatgaaga ttgtcctcca
    36601 catcataagt tgactcaaaa taatcactgt ccttaaaatc actatcatat ctatccggaa
    36661 taccgtcatt atcaaggtct tttgcaaacg gatcatagaa gttaccctca tcgtcaatat
    36721 caagtcccgt tgctactctt aaatcttcag aatcaacata aaccaaatca ctaaatgtag
    36781 ataactcaat ttcatttttc atatttctta gagcttcatt ttctccctta ttttcataat
    36841 caaagctctc tgttttaata ggcgtatcat caatgtactg tgtccaagtt ttctcctcta
    36901 aattaagctc atattgaata ccatgtcttt catccggtgt attggtatag gcgataccga
    36961 tatgctttaa gtcaggatat agagtatgaa actcatcata actgtgattt tcttcatact
    37021 ctctgttaca gaagtcaatg attgctcttt ttacatcttc cacaagagga ttatcatttt
    37081 tcttttcttc cacctctccc atatcgagca gcttatttaa ctctgcaagt cttaatacct
    37141 tagttttcaa ctcatctgcc ttttcaaaag gcttttttaa ttcctctttg gcattttcaa
    37201 gctgttcttt tgtacttatg agcttttctt caagcatatt tagtttctcc ggcattttct
    37261 caagggcatt atccagtctt gtgatattac cgtccgcact tgttccaagc tctcctaaat
    37321 gctttgcaac accgtttaag ctgaaattat gagaattagt aaagaagtta tagcttacct
    37381 ctaaatccat atttctatac tttcctataa ccttgctttc attgattttt acctttgaaa
    37441 tagcttcaag taatctttct ccggctaatt tcttatcggt aattttttct cccgcaatgg
    37501 taatagaagt aaacttttct tcaccttccg ctttaggctc tacattggat atatcttttt
    37561 tgacagcttc tatgagcttt tctgttcttg cgatttcttc aggatagttt tttgccacct
    37621 tatcctctaa tctgtaacgg ttagacttat agtttgcttc aagcatttta agttttgtaa
    37681 cctcattatc caaatccatc ttttctttaa tctttggatc acccgttgcc aacgctttaa
    37741 tttcggcgta atttaagctg ctttcgtcca catcttctgc cacacgaaca ggagtcttac
    37801 tggtcataat ctgtgaaatg aacttttgct tattctctat cgtctgccaa aggtaggcat
    37861 caaaggtatt ctctgtaaca taacgataga tatttacctt ctcattttca tttccctgcc
    37921 ttacaattct acccgcacgc tgttcaagat cagctggtct ccaaggcaca tccaaatcat
    37981 gaagtgcaat cagtttattt tgcacattcg ttccggctcc catcttctgc gttgaaccca
    38041 ttaagatacg aacttcgcct tttcttacct ttgcaaagag ttcatccttt tgcttatcag
    38101 aattcgcttc atggataaag gcgatttctt ctttcggtat tcccattgct acaagttttt
    38161 ctctaatatc atcatagata ttaaattctc catctccttt tggagtagac atatcggaga
    38221 aaagtagctg tgttgaccta ttttcttttg tcttatccca aatagcaaat acatttttta
    38281 cgcatacatt tactttacta tcagggttat caggaagtag gggattgatt aaacgctgat
    38341 ctaaggcaag tttcttaccg tcattggtaa tcttaagcat attatcaaca tccggctcta
    38401 caaccctatt tctgacatca tccgctcttt cagaaaggct ctttaggatt tccttttgtt
    38461 cttcagaagg ctcagtttta ataacttcat aattagcttc aggtgtagga aggttaagca
    38521 tatccgcagt ctgaatatct gctacttcct tgaacatact cattagttca ggcaagttat
    38581 agaactttga aaatctcgtt tttaccctat atcctgttcc ttcaggagat agttcaaagg
    38641 agctttgtgt ttctccaaaa gtggaagccc aagaatcaaa atgctctaag tgatttttct
    38701 ttaaactctc atactgaaga tacctttgca ttgtataaag ctctgtcatt gagttactta
    38761 caggtgttcc tgtggcaaaa actatgcctt ttcctcctgt catttcatcc atatatctgc
    38821 atttcataaa catatcggag gacttaaagg cttctgactg cccgatacct gcaacatttc
    38881 tcattttggt atagagataa aggttcttgt aattatgtgc ctcgtcaaca aagagcatat
    38941 caactcccag ttcctcaaag gtaattacat cgtctttctt aaaatcatca tttaatttct
    39001 caagccttgt ttccagtttt ttctttgtct tttcaagctg ttttactgta aagttctgat
    39061 ttctgtcatg cttgtattct tctacatagt ttacgatttc attaatctga tcttggatgt
    39121 gcttttcctg atattcctta ctcatcggga ttttttcaaa ctgcgtatgc ccgataacaa
    39181 ccgcatcata ttcccctgtg gcaatctttc cgataaatct ctttctgttc ttcggttcaa
    39241 agtctttttt atcagccacc ataatattag ccgacggata tagctgcata aattcacgac
    39301 cgatttgtcc tgttaagtga tttggaacaa caaacaaaga cttactgcac attccaagtc
    39361 ttttactttc cattgcggaa gctaccatct caaaggtctt tccactgcct accacatgag
    39421 caagcaatgt gtttcctcca taaagacttc ttgctatggc atttctttga tgaggtctta
    39481 aatcaatttc tgtattcatt ccctcaaaag aaagattgct tccatcatat tctctgtttc
    39541 ggattgagtt aaaacgctca ttatacagtt ttacaagacg gtttcttctt tcctgatcgt
    39601 taaatatcca gttcttaaat tcttctttta atagctcctg cttttgtcct gcaagcatgg
    39661 tttctttttt atttaagaca gaagttttag aaccgtccgg atttataatc tggtcaaata
    39721 cctttgtttc tttaagattt agggcatctt caatcagctt ataggcattt acccttgatg
    39781 taccaaaggt catttctgca aggtcatttc ctttatccct gctttttccc tctacattcc
    39841 attcacttgt aatatttgaa aatttaacct taatatccca tcttgcatat cccggagttt
    39901 ttaaagtttc aaagatgaat ttttcaatat ctttaatcgg tatccaagtc gcaccaagac
    39961 gcacattgat ttcacttgct tctaattcct ttggaagaac tttggtaagc tctgcttttt
    40021 gatattccaa acgattcatt tcatagctta tcagctcttt ttcttttcca ttctcaacaa
    40081 agccaagatg aggaagctct cgttccgttt gtcttaactt tgcaaggtag ctgtctacaa
    40141 tggcaatctt atctctaata tttccgctta ggtactcatc ctttgttaca tagccgtact
    40201 tatatgaatt actgccgttt gcacaagcga aaggcaaatc tccatcttca aggttaaagg
    40261 ataatggtct ataaaagttt tgttcttctc tgatgtttag ataaatttct cctctaagtt
    40321 cttctatcaa agttggtctg tctttacctg taaggctttc catatactca aaatccacat
    40381 agcctttttc agataccgat aagacaagag cttcaaggga agtgtccaca tggtctatga
    40441 ctttggcttt tgtaatggtt ctctttgaga aaatatcccc ctttgccttg acgttttctt
    40501 cttcatcaag aatttcaatg gaagatacaa gagggaaatt gctatcctct tttaaggctc
    40561 tggtattgct taaattattt ataaagccat gcttctttga aaagttgtca tagacttcat
    40621 taagcttttc ttgagctttc tttacctcat catcggaata gtcctctttc tgcttgtaga
    40681 taacatcttt aagggcagta ttcagttcaa gatagtcctt aatcttttcc ttattcttat
    40741 ccgttatctc tttcttgatg aaaagggagt tttctctgta atagacttca tcatcaataa
    40801 cggtatagga aaagtttttg acatcatccg ttgcaggaat tgaagtgatt tcatcatcta
    40861 aaagctctat ttcttcataa gttgatcctt tggatatttc ttcacttgct tttgtcagaa
    40921 gctcttttaa gtcggcattt tctttcggca ggcacgcaag tgtgtttcca aatcttcctg
    40981 aaacttcacg cattgtgccg agtacctgct caggatgatc tacaaaatat ttgttatagg
    41041 taagaccatt ttcatcttca gcaaggtgaa tccagtcctc atctctttct ctgatactgt
    41101 cccttttctt taggaatata atatctgagg ttacttctgt tccggctatg cccttaaagg
    41161 tatcatttgg aagtctgatt gcccctaaaa actctgctct tgctgcaaga tagcgtctta
    41221 cactttcatc ctttttatcc attgttccac ttgatgtgat aaaggcgata ataccgccgt
    41281 ttctgacttt atcaatggat ttcgcaaaga aataatcatg gataaggaag ttatttttgt
    41341 tatactccct gtcatttacc ttatattctc caaagggtat attgccgata accgcatcaa
    41401 agaagttatt ggaaaaggag gtttcttcca gtcctttaat ctgcacttca ctttcagggt
    41461 atagtagttt tccaattcga ccgcttaccg agtcaagttc tatgccataa aacttggact
    41521 tattcatttc atcagggata ttgcctataa agtttccgat gcccattgat ggttctaaaa
    41581 tgtttccctg tttaaatccc atatccgaaa gcgtcttata tattccgtca attacggttt
    41641 tcggtgtata aaagctcgtt aaagtagatt ctcttgcagc ttcgtattct gcatgtgata
    41701 agttctcatt tagaaaagcc cttgcttctt tccactgacc ttccttgctt tcatcaaaga
    41761 cttcggaaag tccgccccag cctacatacc ttgctaaaac ttcctgagct gtactgtcaa
    41821 gctctctttg tccgctctcc actctgttaa gcatggaaat tgcttcaagg ttattattta
    41881 acctttcgct tggacttaac ttttcaggga gcgtttcttc tgtaatttta aagttatgag
    41941 cttccgcttt ccttatttga gtttcctcag tagtctgctc tggctgttta tatgtcaggt
    42001 tttcaaatac cctctcaagg tcgctttcca gacgataagg aatgacatcg aagcttgtta
    42061 tcatcccgcc aaaatactcg gtgttgtcct tcaccgttac cgtatttagg ttctttctca
    42121 tatcatcaaa gcgtgttatg gtatggtttt tgtccttgta tttaacttca tcaccgacaa
    42181 taaagttcgg tctttcaaga cttattttat tcagcaagtc ctcattatct gtaaagtcta
    42241 caatcggaat ttgatggttt ccacttctta ccggatcaag ccacaaatca tatcttcctg
    42301 tgatttcatt tttagaaatt tctctgactt tatattcttc atgattaaaa tagacggtat
    42361 cgcctgcttt aactgcaaac tcttctaaaa ggctgtcctc tttttcattc agttctactt
    42421 cttctctttc tttgaggtaa tcaaataagg tagcttgaac tgtgccacgc tttgtagctt
    42481 cttctgtttc ttcaggtgct tcaagctcta ctttatcttc aaagctaagt tctccattaa
    42541 agacaaactg aagctcctgc tcatccattt gttttataag ctctatatct ttaagttctc
    42601 taaaggtctt tggaaaatcc tctaaaatga gcctctgggc atttaattct ttcagttcct
    42661 caaggttaaa atatccccat tcaggctcta ttccaagaac aagtccaaag gcatcaccgc
    42721 tttccctgtc atactccgtc atataccaag tccaatttga acgaaaagga ataatatatg
    42781 ctgcatgaac ctgcttatcc gctaaatcta catcctcctg tgcgtaaatc tctggcactc
    42841 tttcaagaat ttcatcaggc attagatttc caggatcatt tttagaataa tattgcggtt
    42901 cttttacttc ttctatctct gccgtacttt ctgtttcctt ttcctcaaaa taaagttttt
    42961 tagaaagcag ctcatcaata tgctttgcaa cacttgtcca agtaaggaac acatcattac
    43021 aatcattctt ctgtaattta aaaccctttg catcgtgcca ttcatcactt cccattgcac
    43081 ctgacacaac gtgagaatgt cctccgattc catattcatc ttttaggaaa ttggcttttt
    43141 cctgcaaagt atgattttct ttaaaaaact tggtgattcg ttcttttcct ctatcaacgc
    43201 cgcttcctct tgaaaggctt tcaaggactt catcttctgt aataaagagc tttaccttcg
    43261 gaagttctgt cagattggta ctatattcct ttcgttgcag ctcaagttct tgcaacttct
    43321 gataaagact atctaccttg tgataatgat accttaatac atttctgctt tctttgtatc
    43381 cttctaaaaa cctgctatat tctttgattg tttctttcag atattcagga ttttttaatg
    43441 cttcagataa tcttttcgtt tcttgcggaa agccaccacc tctttcaaaa gagttgaaat
    43501 atccttgctc tattccttct tcgcttaaat cgtgcgttag ataccataag gattctgaaa
    43561 ttctatccct ttcatagtcc tgtgcctcta aaagttctac atttgtagca aactcacctc
    43621 tgtcaagaag ttcatttatc ctacttgctg catcactcca acttaaaatt tgtgtattat
    43681 cctctcttgc cgatgttcca taagctaaat gaataccttt atccgaatac caggaagata
    43741 cttctctttc atcaatgtaa aatccgtttc cgcctttaaa ggtatctttg aggtattctc
    43801 ccaattcttc tatgctcttg ccttttgaaa actcagcaat aacaggaagc ctaccgccat
    43861 catgatttcc tccgttaata aggacggtat ctatatcctt ctcagttagc ggaatagtaa
    43921 ggcttatctg tccataagaa ttttcgggta aagaaaaaga agccttttca gcttctatta
    43981 tccctttatc cgtattgtct tttaaatctc cactacttcc ttgatggtca tttctttgag
    44041 agctgaaatc atcgccccat actgcggatt gttctcgtcc tctatcttcc aagctgctat
    44101 caactttggc ttctctgctc tcataaactc tactgcctgt ttctgaatat ccatcaggtg
    44161 tcctgtcagc ttcttctcct tgtatagatc caaaagcatc tcgaagtggc tctgctcctc
    44221 gctctgtgaa aggtaattca gcctcatcac tgcgtaggtc ggattcggat acttccttat
    44281 aaagtccgtc tgttcctcca agctgtttag cgtattctcc ctgatttttt ctattatctc
    44341 gtccgtactc tccatttcgg agaactcgct cgttttcatt tctttcctga tcatctcgtc
    44401 gaaatacatt ttcttctacc tcctctaatt cttctttaat tttattatat cccgcttctt
    44461 ttcctattaa aacttctttt tgaagctcaa gctctttgct tttttgaatg gtttcatcaa
    44521 taatctttcc gctaatatcc gatacacttt caccaaggct cattaaggat atgctgtcaa
    44581 atctttgaaa attttctctt aaaagctcat aatccatcgg ataatctaac ttaaatcttg
    44641 aagctaccgc atagcttacc gagtccctta caaacttggt aaatgatatt ctatcctcat
    44701 ccgctactct aagttcattc attaaggtat ctattttttc atcaccgtag agtctgctta
    44761 aagaaaagat gttttcaagt gtgctttcac tttcctcata gccctcgctt tttatcattt
    44821 cctttaagac atcttgatgg gcttctttat caaatctcca aagattgacc tcgttgacat
    44881 ccctgttttt tgaaactgtc tggcttatat caaagatata gtccaccttt ttatatgtgc
    44941 cgtaatcctc taaaattggt atccctttct gccctcgcat aaccgttcgg ttaaagcgtt
    45001 ccctccagta gtcgaacttg gcacaggctg tcgcttcagg atttttatca tatatactta
    45061 attgacttct aaaatcatac ctttgattgt ttccgacaac ttttaggagc tttagatatt
    45121 ctgcttcatt atacaaaaca tcttgtttta ttagctccaa aatgttatga aaatcattta
    45181 ttcgcatttt tacctccttc ttttttcaaa caaaaaaggc ggttagattt tactctaatc
    45241 accttcgcta tataccaaat attattactc tatttcaatt ttttaattga ttcactttca
    45301 gatagtatct tcatttgttc aatagctatt gcatttaatt tttccaatct ttcagattgg
    45361 ctcatgcctt catttataaa taccgcatta agattctcaa gattagaaag gcagacaagc
    45421 tgtgatatat ccgcataatc tctaatattg ccttttaaat ccggattttc atcacgccac
    45481 tccttagctg ttattccaaa tagagaaaca ttaagaatat ccgcttcact tgcataaata
    45541 aaattgattt tttcttttga aagtttatcc ggtacaagtt tgtttttaat ggcatctgtg
    45601 tgaattctat aattgatttt tgccaaattt ctcttaatat cccatcctaa cttcttttgt
    45661 tcttcttcct ttaggcgttc aaattccttg attaaatata atttaaagta tggcgatacc
    45721 caagaagcaa actcaaacgc tatatcttta tgagcataag ttccaccata tcttcctgct
    45781 ctcgcaacga taccgataga attagttttt tccacccatt gcttgaccga taatataaaa
    45841 cggttaaatc ctgcttcatt tttaattccc tcgaattcgg gggaattaaa attcgtgtta
    45901 tacatttctt cccaaatacc taaaaactct attgtattct tgtttctgag ccacttttca
    45961 atcaaagcag aaccgttctc aatatttcta accatatccg ttaaagaaat ataatctcta
    46021 ttgtctatgg caataatgct gatttctgaa ccttcaacat ttattttaga catataatca
    46081 cctctcaatc attaaattga ttactttgca ttatagcatt tttagagcat tttttccacg
    46141 ctgaaattag tgctgtctta cttgcgatat gtcaaaaccg cttcttttat ctcattttca
    46201 atcgcatcta aaaattcttt cttagaggat ttaccctgag caatatcaga taattccatt
    46261 tcccactttg ctgtcgtttc tgccgactta aaggtatctg ctacaatcgt tacaaggctg
    46321 attcctttat gcgttgcaat tagattcttc ttatctcttt cgacaaatct cttaaagatt
    46381 agattttcaa taattcctgc ccttgttgcc ggagtgccaa gaccttttct ttccacctct
    46441 acaccttttt ctaaggcatc atttcccgca atctccatag actttaagag cgtatcttca
    46501 gtaaagtgtt tcggaggttg ggtaaatttt tctttaacct ctttattttc aacgctcaaa
    46561 acatcaccaa cacttacatc gggcaataca gcatcttcac tcttttttga tttgtattct
    46621 ttaaggtatt tgctaaagcc ttcatcctta attaccttac ctgaacttgt aaattcaaaa
    46681 ccgtcaaatg aagctacaat ctttgttgta ttttcaataa gcggatagcc tacacttgca
    46741 tgaaatttat cggatataag gaagtaaact tttgcttcac ttaaaggtaa ttcagaaaca
    46801 tcttctttta ctgaactgat tgtcggtatg attgcatgat ggtccgtaac ctttttagag
    46861 ttaaatacta ccttgatacg ctctgtatca aagtcatttt tacccaaaat gttgttgact
    46921 gtactcacaa tcatatcctc tgttaaacat ctgctgtctg ttctggggta agtaatcagt
    46981 ttcttttcat acaggctttg agtatagtca agtgtctgct tggcactata tccaaaatat
    47041 ttattgcact ccctttgaag cgtagttagg tcaaaaggta aatcaggctt tgtaattttt
    47101 tctttttgaa taacatcagt aatttcaatc ttatcgccta ctaaacttat aagctgttca
    47161 gcagcaactt catcatcaat tctgtctgtg gagagtgtaa atccattcat agaaagctct
    47221 actgtgtaat atttttcttt ctgataattt gctatctcat catctctttt tacaatcatg
    47281 gcaagggtag gtgtctgcac tctgccaaca ctgtaatttt gctgatacag acaagagtag
    47341 agcctactaa tattcattcc gactaaccaa tccgcaatag ctcttgcctg tgccgattca
    47401 aagagattat catagtcttt tccgtctgtt aggttatcaa aaccctcttt aatggcacta
    47461 tcttccattg aagaaatcca aaggcgtttc atcttctttt tacaattcac ttgattatat
    47521 acaagtctaa aaatactttc tccctcacgc cctgcatcgc acgcattgat aaccgtatca
    47581 acctccttat cattcatcag ctttttaagg atgttaaact gcttcttggt tgcctttgct
    47641 acttcatact tgtagtcgct cggaataatc ggtaaatcag ccatattcca cttggcgtac
    47701 ttttcatcat aagcatccgg atttgccatt tgaattaggt gtccaacgca ccagctcacc
    47761 ctatatccgt ttccttcata atatccgtct ttctttttat ttgctccaat aacctttgca
    47821 atggagattg caacactcgg cttttctgca ataactaact tcatctgtgt tttcctcctg
    47881 ttataaatca aaatagggcg aaagatgtta ctctcccgcc ctaactttgc acccaaaata
    47941 atatgttttt aggtgcgttt attcttcatc ttctacttca tctgcatcat ttatttcttc
    48001 ctcgctttca gattcagaaa agaaatcatc atcttcctcc tctaaggctt caagttcttt
    48061 atcttctttc tttttcacaa ccttaaaata gtagcctgct cctaatgcac cacctactac
    48121 aagaagcaaa ataatatagg ttcctaaatt actcttttcg tccttcttct caggctttac
    48181 ttcttcctta acaggttctt cttttactac ttcctgcttt ggtgcttctt ttttctccac
    48241 catgttaagc aaatcatctt cagatacttc cgtaagaagc attacattct cactatcttc
    48301 atcatgattg atgattaaat gaaaagtctt accgtttttc gtttgaaagg taataaactg
    48361 ccttgcatct gccgaatact gatccgtttc tttgttatcg ctgctatctc catgatgaat
    48421 cggatactcc ttatttgcat tatctttatt ctccgtaacg gatgctctcg cctttgacgg
    48481 agatgaagct actcccttgt tggtgtttac actcttactt gcagacccca ttgatgaatc
    48541 ctgactgtta ttcgcaggag cttttgccgt caacttattg ggatagcgaa cttccttttc
    48601 tttctccttt acttccgttt tgcctttacc ggtatccttt gtagtattac ctgagcttac
    48661 cgaatccgat gagcctttac cggtactact tacagaagtc tgtggtgttt gaggagaaat
    48721 acctgaactt gtcttaattc cggaaatagg actaatcggt gtaactgatt gtgatgttac
    48781 aggagcttta tttgtttcag ttgccttttt ctcaagctct ttcacctttt ccgtcagctt
    48841 atctatttcc tttttcatat cttctgataa gtccttattt cccttatctt tcttcatttt
    48901 ttctttcagg ctatcaattt catcttcaag gtctttaatt ttattctttt gcttatcact
    48961 taatttgtct ttgtccttaa gttcgcaatt taacttatca agtttatcct gaagctcttt
    49021 tgcctctttt tctatttttg aaatatcgtc tttagaaagc tctgtctgcg ttcccttatc
    49081 ttctgtctga gtgcattcat cctgctttgg cttttcttct tccgtctgcg tttccttatc
    49141 cttaacctcc atctttgcta ccttacggat aatctcatct tttccatctc cctttacctc
    49201 ataaaacaga aattcatcaa tgaatttcat atcatctggg agcattggaa gatctccgct
    49261 atcaataagt tgtcctttct cagcctttat cttttcttcc ttaaagacct tttcatcttc
    49321 aaagatatac cttacattta cttcaacttc cgtttgaaca gcttcgtttt ttgttggagc
    49381 ttcagatttt tgctcctgtg cataaacaac cgtccaaaga cctaaaatac aactaatact
    49441 tacaattacc gcaagtgcta ccgtccaaaa cttattattc tttttcaaac tggttttcat
    49501 ttgttttctc ctttttcatt tctgcttttt gtctgtttac ctttgctatc aaatcgctga
    49561 ttgtgatgtt gttctttctg caaatggcaa tgatttcttc attttcaagc tcttcttcac
    49621 gaatgaataa aggctccaat tcttcatcaa tcagagcctt tttatccttt aacttcttta
    49681 ttctgttttt tactgttgta agttcctttt tcaaattgtt acctcctatt tctttacatt
    49741 tggcggaaaa ccgaatccta cgggatggtg cttacaaaat gttgctatcc aatcatctaa
    49801 ggtagttact cttattcgac cgatgttatc aatcactttt ccatctccaa tgtaaatacc
    49861 tacatgacca taggtaagcc ctgcactgct tccgctactg ctgctttcaa ctgccacaag
    49921 cattcccact tttagctttg acctgtctga tgtaaaggta tagtttcggt acatatcaca
    49981 ggcattcccg cctatatatc caagtcctgc attttgatag acctgtgata cccacatggc
    50041 acaccagcct gcacccggag atggcgtaat gtatgcagca ttaacaatct tcttttgtac
    50101 ttccgtagat gcctcatact cttttccacc gccgatacct ccatttgcac tgataaggtc
    50161 agaattacca aaggcttctc tcatattgcc ttgtgcaagg aacaaggctt catagtgctt
    50221 tagattatcg ggatagtttg caaagacctt tcggataata ctgtccatct cttttttatg
    50281 aagcgttacg atgagttttt tatactcata aggttcttcg tggctttcgg tatattcatt
    50341 gccatcttca tcggtatagg tttccgtaac cgtcctgtat ctgatttcaa tttcttccct
    50401 atactcaagg tcatacatgg actcaaataa ttcttttaat atggattcta cttccgatac
    50461 actctttacc tctccgcacc ttgatgtaat gtaggataaa agctcatgga cattatgacc
    50521 gatgtattct gtattattta agatatattc atcatatcca ggataatttt ctttcacata
    50581 gtcaacttca ctttgaagct caccctccat atctgaaaat ttctgattga tttcacttag
    50641 gacatttggc tttgataaat agcttgttgt aagcacagag cttgtagagt tcataaagcc
    50701 tgtcattccc gttcctgcaa agttaatcac aaaagtccct aagataatga tacctatgaa
    50761 aataagcatt agtccttttg cttttcggat aatcatatcc tttgagcttt tcagtgttcc
    50821 gataagtccc tctttaattc gatctctaag ccttgactta ttctcacgag aaattgctgc
    50881 ctttacctga tttttctttt gaaatctttt atatgcactt gctcttttat actcatccgt
    50941 ctttttcagt tcctttttgg catcacgaaa ttccaacttt gacttacgct ttctgatttt
    51001 atagtcctta tttgtaaggt catagcctct tttagctctt ttcttatcag agtacttctt
    51061 tatgccatga atgagcttgg agcttgtatc tgctgtcttt tctcctgctt ccactccttg
    51121 attttcatca cttccatgag acaagtaatc tcttacagtt tcacttcctt tagcaagtcc
    51181 tgaaagggca gataccttta ccatgtcttt ttttagcttt ttcttttggt cttttgatag
    51241 gctgctataa acttttccgc tgacagcatc ttttcccgcc tttttatcat cagccttatt
    51301 tccctttggt gcatcgtcct ttttccttgt ataaaggctt tcagaatagt tcttcctctt
    51361 gtacctgctt ttttgagtct tatgattttt aaaggaattt tctaatgatt tttctttatg
    51421 ggaaagatta tcctccacat cataggtcga ttcaaaatag ttgctgtctc taaagtcatt
    51481 atcgtatctg tctataatcc cgtcattatc aaggtctttt cctaaaggat catagatttt
    51541 tccatcctta acctccgtaa tataatctga tcttcccgtt tcaattgcgg aatttacgtc
    51601 tcccttatct gaagttctat atttttcgtt tctcttggat gttccataag acttttcatt
    51661 atcagcactt accttacttg ttttttcatg cactttatcc tgaaaccgtt ctttctcatg
    51721 aacgatttta cctctgtaat catcgttatg ttttagctta ctttcttcag gtatcgtatc
    51781 tgttttacta tgaaacatct ctctttcaag gcttgcctta tgcctttcct taaaatcctt
    51841 tttcagcttc tttcccatag gctacctcac ttcttccggc ttggtagtca tcttctgata
    51901 taaaattgta tctttcggaa acttatcaat gaaaggcaca atggtatttc caaagaataa
    51961 gagtccctca ccttcatttg aattagtaac atatctgagc tgtggaagtg agattttaag
    52021 ttttcttgca agtatctccc tatctcctga tgcttgatta agcattaaga caaagtctgt
    52081 attgtcaaag atattttcta tctccttact cataagtagg tctttgacat tctgcgtaat
    52141 acctgttgga atacctcccc atttacgaaa tcttttccaa atctctacgg aataggatgc
    52201 tgtctgctca tcttttaaaa gcaaatggaa ctcgtcgata tagtaccttg tagccttgtt
    52261 tcctctgttt tgtgacacct tattccacac ctgatcctga ataacaagca taccgatttt
    52321 ctttaattga cttccaagct ccttaatatc aaaacaaagg agcttcttat ttaaatccac
    52381 atttgacctg tgattaaaga cattgagaga ccccgataca tagatttcca tctctgttgc
    52441 cagcttctta ccgacctttt cttcctgccc ttttaacata tcgtataggt cttgaagtat
    52501 cggcatattg tcaggagtag gatgttcaaa atacttttca tagattttag gaaggcacct
    52561 atctataacg gacttttctt ctgctgtaag accactaccg cctactacaa gctcaagcat
    52621 actcatgata aagtttgcct tgtctttcag cggtgcatcc ccatcaccgt agttcatgtt
    52681 tatatcaaga ggattcaggt aatccttcga cttactgctg accttaatga cttctccctt
    52741 aaactgcctt acgaggtttc catactctcc ttccggatcg cagataatca catcatcatc
    52801 tgttactaaa atcgcatttg ccatttctct tttggcacta aaggacttac cgctgcccgg
    52861 agttccgagg attagaccgt ttgggttctt taatttcttt ctatctgcca taatcaggtt
    52921 atggcttagg gcatttagtc cgtagtaaag actattactt gaattgataa aaagctcctc
    52981 tgtggtaaat ggcataaata ctgccgttga tgagcttgtc aatcctctat ctatttcaat
    53041 cttatttaca ccaagaggca gcacgctcac taaaccttgt tcctgagaat ggtcaagtct
    53101 tttcagctta cagttatgct tatttgcaat ggagcttatt tgagcaattg tgttatcgag
    53161 cttttgcact gtccttgcaa agttcataaa gacgatactt accacaaaca ttcgctcatc
    53221 tcttgtctgc aagtctttta agaggctctt tacatcctca ccataggtaa ttaagtccga
    53281 tggaagaatg tccatatcat aaccgcttct taccgccttc ttattttcct caattttcat
    53341 cttatcaata tcagtgtttt ttcgttttac catcttaatg gcttccgatt ggtcgattgc
    53401 cctaatatga aaggaaatat tgatgttatc gtcaatatcc aaaaactcgg ataacatcct
    53461 atctgaaagc tcactggcaa ggatttgaaa atgacttgtt gctccgatga atttgccgaa
    53521 cttaaagtat ctactcggtg taaagttaaa ctcatcaggc acaatatatg tctttgtgct
    53581 ttcccttttc tttaggtctt tgtaggaaaa tttaaatgtt ttatttggat ttaaaacatc
    53641 atgaagtatc tttagccttt cttctccgtt taggctttct gctcttactc ccatactttt
    53701 aagactcgat aatatatcta tctccagtct ttcaagtttt gatgttgcct gttctaaatt
    53761 atccgcttcc actgtaaagg ttacatactt tgattttttc aatccgttat ttccctttac
    53821 aatctggctt ttaagcatct ctctaaattc aaagcgtata tcgtcgaaac cgtccttttt
    53881 atccggtatc tgaattgccg acttcatttc ttcgtttcgt ccgagctgat tgatatatga
    53941 aaactcaatg ctgatacttg gatcaaaaga gtttaggaag tttgcaaatt gattaaaaat
    54001 caagtcccta tcttcatcta aggcaagctg atagttaatg tcctgaaagg ctatactttt
    54061 actgaagtgc ttttcatcaa gctggcatat tccgcttttt aaaagcctta gatagggaat
    54121 ggtatcttct accgtatatc ttttcggttc tttcttaaag ataaggtcaa ggattcctcc
    54181 cttatctttt tttgacttgc aattactttt ctttaagtcc cgcttttgac ttctcaatgc
    54241 cttttcgttt tgcatgagtt ttatttgctg aatttttctt ttcttgttca aggtaaacct
    54301 cctttctcac tctcttttgc ggttgataaa acttatgaag ataaatgtac ttaaagtatt
    54361 tctcaaatgt cagtccgtct ttttcaaata gtgtgatgaa aaagatagga agcgtggata
    54421 cgataaggaa tatgacagct atgtcatttg gcacaaactt cctcataaat aaatagactg
    54481 gtattccaac cagtcctgca agtgtgaagc ctatgagctg ccttttcgtt aggttaaaag
    54541 caacctttgt ctttacccta tttaagtctt tagggattgg tacatatgcc atatcttacc
    54601 tcccatcttc tttacccttt gacagctcct caaagtgttc atatacagag ccgatttctt
    54661 cctgaatagt cgcaagctgt tcgtctgtgt tcttgttata tctttgctgt attaggtaaa
    54721 aatcgttatg gcagcgacca atgtcctctg ttttttcttc caattcttcc accttattat
    54781 gaagcctgat cctatcaatt atcgccataa tacctgctcc aattactact ataaatactg
    54841 tttttctttt gttcatatta cccttccttt cttttagtgt gcatttaata cgcttttggc
    54901 cagtgttccg ctctttaaca tcattaaccc tagcaaaacc gcatatccaa gtatcgtaaa
    54961 ggtacttgtg tgtatatctg ttatctgtat tgtcttaact aatactgcgt atattccaag
    55021 acaaaccatt aaaaagagtc cttgtaaacc tatggcaaac aatcctttga tatagtttgt
    55081 tccaatctgt ccccactctt tgtttcccat tgtggcaaat ggaatggctg aaatggatga
    55141 gtaaacataa atctcaaaca ttcttccgta aaccacaagc ataatcacaa ttgaaatgac
    55201 ctgtattgcc acctttataa gcgaggtttc aaagagtatc atgacaagct ctccaagacc
    55261 tttatccttt aatccatcta ccatttttac tatctgatct ccggagataa cggcagaagt
    55321 attgattacc cctgccgctt tatttaccat gtgctgtgcc acatcaaaga ccgccattga
    55381 aaactcaaat gcatgtgata ctaaccatac agcaatccac atcttgatga tgtacttgaa
    55441 aaattcaaaa gtatctgtat cgtgcatatt gttcttttgc attaccatat tgataagctc
    55501 gatacaaagg actgctgtga tgattagccc tgctatggga ataatgacgg aatcgttaat
    55561 gcttttaata aaggcaaata catctccgtt ccaccccata ggagtttttc ctacatctgt
    55621 tgcaactgca ccaactttat cgttgatgtc aagaaacatg gactctaagt ttgcttgaat
    55681 accgcccagt agaagctctt taaagaattc ttctatcttg tcgaatattc caaacatcta
    55741 agctctcctt ttcttattac ttgagtacat ttgcaagaag cggaatcagc ttaagtccga
    55801 taaggacaat gcctcctccc gccataagct gcttgatgcc ctgagatttc gctcccggat
    55861 tatcattacc atatccttcc ataaggttaa taactcccca tgctccaagt ccagcaccta
    55921 ctgccataac taaaatcttt aatacattta ctgcctgtgc gaaaaattcc ataatttatt
    55981 cctcctcatt ttcttctttc ttctcaatct tgttatatga cttcactaca aagtttttgt
    56041 aagtctttcc ctctttttca cgcttcttaa agtagccgaa tacatgaatc aaatcgcctt
    56101 tttcaaagtc ctctgctttc tctgcttttt ctccgtaggc tgcacaattg atgtactcct
    56161 tgccctttcc atattttttt acaagcgtaa agtttacaac ttctacttct tcaccctctt
    56221 tttcaaaact tgagaaagta ggttctgcca ctaagttggc gttgatgtta atcatttctt
    56281 gtttcatctt tttaaatttc tccttttcat ttcaataaaa aaaacgactg gagtttttct
    56341 tttccaatcg ctgatactct tactatttag ttttcgttta agtgggattt ctgatcctta
    56401 tcatcttgcc tcctgatttt gaataaaaaa acagcaaatc tttttaagac ttactgcatt
    56461 cttgtaataa ccgtctctct atttaatttt gcttttcctt ttcgcttcat atagctttct
    56521 atgtcaaaca aattcttctt gtcataatcc tcaagcagct tataattctt gtgctttgta
    56581 atatcgtatt tatctgaaag aaaaggtctg acacctctaa gctgaaatat acattttcca
    56641 ccgtccatta cagtaatctc atcttgactc atcagttcct taccagtctt ttgatagttt
    56701 aaaccaaagc tcttttgatt tgaccttgtt tccgacgtgt tatacaggtc gatagtttct
    56761 tttcccagtg tttcagacaa ttcttttagc gtagttttct ccttgccacc aagaaacaag
    56821 gtactatcac agttacctac gattgtatca gcgttatcct tgtagattgc ctttagctga
    56881 gattgtgctt gaagtatgat acttgctgaa atctctcttg aacggattgt tgcaattaac
    56941 ttctcaaact taggaattaa gccgatatta gcgaactcat caagtagaca cctcacatgg
    57001 acaggtaatc gtccgccata tacatcatcg gctttatcac ataaaagatt aaataactgt
    57061 gagtacataa tggatactac aaagttaaat gtatcatcgg tatcggagat aataacaaat
    57121 agtgctgttt ttctatctcc cagtgtatca agctcaagtt catcgtcact catcaagtcc
    57181 ctaagttcct gaatatcaaa gggagcaagt cttgcaccac aagaaataag aatcgatttg
    57241 gcagtttttc ccgcagccaa tttgtacttt ttatattgct tcactgcaaa gtgtgtaggt
    57301 tctttcttct ccagtgcttc aaagagccta tctatcggat tcatataggt ttcattatct
    57361 tctctgactt ctgaagcatc aatcatatca agcagagttg caaagttctt ttcttctctt
    57421 ggagcttcat agaagatata gccaataagt gctgtatagt agagtttctc agctttcacc
    57481 caaaaatctt cacctgattt ttctccctct cccttggtgt ttgcgataat tgtctgtact
    57541 aatttgagta tgtccttttc acttctaaga taagcaaagg gattgtattt catagacttt
    57601 ttgaagttaa tggtatttag gatttttatc tcatatccgt tatcttcaag catcttaccg
    57661 cactcaagga ctatagttcc tttaggatcg gtcacacaat atgacgagtg catctgcata
    57721 aggttcggtt ttacataaaa tctcgttttt ccacttcctg atccaccgat tacaagtaca
    57781 ttcttatttc ttgcatactt tggatttgcc ggtctgccat tcatggttaa tcgttctgtt
    57841 tgcgtaagta ggatattgtt ttgaaacttt tcatccatat atggctctat atcttttttc
    57901 gttccccatc ttgctgagcc atactctttc ccctgtctaa actttttagc atttttccct
    57961 ttggtatata cgataatttt gattaaaaca gctactacca ctcccattaa aatatccgta
    58021 ggatgaatgc ttggaagaaa gctcatggtg ttaagttcca gtataccttg aaatattttg
    58081 tctattacat cgccaccagt atatgctctt acatgatgag aaaagatatt gccaacatag
    58141 aaaaatgcaa gatagggaat gttctgcttt agaaactttg ccttatcttg caccttaaat
    58201 aagcctttga tgtcttttag tatcttatct atcataggct ttgctccttt tgtttatttt
    58261 tgattttctc ttttgaaata gagtttttag ccatctcttt gaacttctca atattcttgt
    58321 gaatagattc tttcctttct gctttctttt ctgattttga aagagcgtgc ttaaaagctt
    58381 tatccatcac tttcatatct ttggcttgaa agaatacaga gtttttacct gtctctttat
    58441 ttttcatcac tgaaaattta acaccgtatc tattcaactc ttttttaagt tctttaagct
    58501 cagcttcttc cactgggatt tcttcaagct gtcccttttt aaccatatct tttagcttta
    58561 cttcatttcc gttggcattg accagttttt caagtcctcc gaatttctcc gcttctttca
    58621 tcaatttttt taataagttt aatagaactt ttccagtaac tttaccagca tttatctcca
    58681 tatttaaggt ctttcttgct atttcttcat tgatcacatc aatatcctcc tctcatatca
    58741 tagcttacaa gagccgaata ataggcatct attccacttg gtgcattata agcggcggtc
    58801 aggacaaaag ctcttatatt tctaatccta tattcatttt ctcttaatac tcctactaag
    58861 tactctaaat gtcctgaatt aagtttccta aatctttcct ttactacttg tacagatatc
    58921 tttgtttggt tgatagtaat gaaagattca tcatctaata ccattacatc tgccagtatc
    58981 tttaatattt catcaaactc ttcagccaca ctcttattcc caatactcat atgttcataa
    59041 tatcctgttt ggtgtcgcaa ttcttctaaa caattttgat atatattttt atagcttata
    59101 tctatcctcc ctcccttcat attctctgaa aataattgat tcttttcttt gatggaagga
    59161 tggatggaaa tatatgctga attatttgta gtattatttg tagtattatt tgttgtattc
    59221 tctggtaatg gttggtgcaa attatccttt tgcattagtg cattttgaac ctgtgcatta
    59281 gtgcaatctg cattattgca ttggctgatt ttgcccttat ccattggtgc attttgccct
    59341 aatgcattgg gcatagaatt ttttaatgct tctgtttgta ttttttgttt tttcaaatcc
    59401 atatccatat ataattcttc aagtttatca ttattaattg tataccattt tgttttatct
    59461 cttggatcct tattgtaatt tccaactaaa agataacctg ctttttctaa ctttgaaaaa
    59521 gttcttttta ctgtatcaaa gctcatatag tcaaagtcat tttcttgcca tgctcgaatt
    59581 gaattatatg tccagtattt accatcatga taattctttc ctgatttttt atttatttct
    59641 atccaataat taatctgctg tagtatgagt gcttcgttta atcctatttc tctagcaaga
    59701 gttttattcg caactatagg ttgttcatca aataaataca taactcatca aacactcctt
    59761 tctttttttc aaacaaaaag acgatagtta tttctatcgt ctaaaatcat atataaatat
    59821 cattttctaa atagagcgta ttgcttcttt cactcccgtt aaaacggcta taacagctgt
    59881 tccaaccatt ggtattccgt atgggcttaa caaaaatcct aatatcaatg cttctatccc
    59941 tatggtaacc tctttttgta aaaaagatgc tattgctcca aatatgcaaa acatcatcaa
    60001 caggtaaagt attgctgttc ctatgccaag taaaaatgaa agaaatgcaa tgagaatact
    60061 taacgctata ctaattggaa ataagataat ttttaatatc catctcatat atactctcca
    60121 tttatactaa atcattttaa aatgtcttaa agcgtccatt atagcttctt ctttttcagg
    60181 cgtacactgt gggatagcct gattgtcttt tttagattta ttataatgct ctctcagttc
    60241 aattccacat ttcttcttaa tctgtgcaat ataaagtgta gaaaccttta gcccaaattt
    60301 ttctaataca tactccttaa tttgagcata tgttgctttg ctctctgcac ttgtcaaatc
    60361 aagctcatcc agcttaattt caacatctat atgcttatcg acatcaagtt tggacaataa
    60421 cgctaccgtc tcgactatcg gtatcgtgcc gggtattcaa aaattgacaa gagaatatcc
    60481 gcaagtcaat ctggcctatt ccctccacgc acctactgac cgtctgaggg aaacgctcat
    60541 gccaataact aaaacatacc cccttggtca ggtactggat acattagatc agcacattcg
    60601 gcaaacaaat cgcaaggtgt ttttggcata catcatgcta aaggatgtca atgacagcga
    60661 cagacacgca gagcaactta ccaagttgtt attcaaacac aaaaagtatt tgccactata
    60721 ccatttagat ttaatcccat acaatcaaac gacagttacg gaaactatgg tgccctcaag
    60781 ccacacacga ataaaggcct tttgccgtat cattcataat gcaggaatta gcgtcaatat
    60841 ccgaacgcaa tttggctccg atattaacgc tgcttgtggc cagttggcgg gggcctaccg
    60901 tgacgatcaa aaacaaggag agagaacaat gtccgctata caagaacagt cttttgaatg
    60961 gctcttgtgt cctgtgtgca agagcaaaac acgacttaaa atccgaaagg acacgatact
    61021 tgacaatttc ccactctttt gtcccaaatg caaaagtgaa atgcttatct gtgtcaaaca
    61081 atttcatata tctattatca aagagccaga cgcacagacg cagagccgat aatccgtagc
    61141 ccttgggtgc gagttatcgg ctcttttgtt ttttggggcg ggtatcaatc aaagttaccg
    61201 ccttatacga tgactttagg aggaacacgg cggtttccat ggagggatcg tcgtgttcat
    61261 tttgcttttt cacactaccc gtgattttca cctgctaaaa aaagcgtagt tctccctccg
    61321 ggatcgtccg atcatgaaca tgaaatattc cagtacatat atgtaaataa aaactcgtgc
    61381 gtccgcatag cgtcatgctg tgcgggcgtt tttatatatt cttaccttgg gacaagttgt
    61441 cccaaagctg gcagccccgg ctgtcgatcc ccgccccctc tccgttcaga acgccatcca
    61501 cccagctatc tgaacggagg aaaatataat ggctatcatc aatttgaaag agttttaccc
    61561 gtggtacacc cacgatgaat atatcgaagt ttccgatgaa gtggccgccg aactgcgggc
    61621 ggacaagctg tacgaggctg cccaccagcg gcggatcgtc cgcaacaaag cgcagtattc
    61681 cctcgattgc aatgatggga tcgaatactc tgcctgtctg cacgagccga ccccgcagga
    61741 gctgttggaa cgcatggatc agttctgcac tctctggaac gctctcaaca ccctgccgga
    61801 gatccaaggc cgccgagtgg atgcctgtgt tatcctcggc aagagctatt gcgaggttgc
    61861 caaggccgag ggcgtaaatg aaagtgctgt gcggcgttcc gtagagcgtg gtttgaagca
    61921 gatgaaaaaa catcttaaag acactctgta agccagtccg aattgatgtg tttgctgtcc
    61981 ttggtatata gagaggattg atcctctcga caactgaata gcggcggctc ccggctttgc
    62041 cggggagccg agcggtgggt acgctgtgac ttctcccacg ggaggacgag cgatccatac
    62101 caacaccgca agtggggcat cgccacccca cggccacgat ccggcgtgga caggctggcc
    62161 gcctgtcccg accggccgcc gcaccccagc gggagcgtca tgtcgagtat cgctgtctta
    62221 tgacgggcca aggaaatgag cgtggcgctc ccaaaatctt tatcaaggag gaaaacctat
    62281 tatgagttgc gatcctgtat tggcggctat gctggcccgg ccgtggaaca acaatgcctg
    62341 ccggggctat gtcatctacg ctatggaaaa ctgtggcttt tctcccacgg acatccggcg
    62401 tgtggtggca gaactccacg aggtttttga cttctgcggg ctggaggagg cccagcagca
    62461 ctttgagaac ggcccctatt gaccttggga caacctgtcc caaggtggag caaggcaaga
    62521 gggcggcacc gccgaggtgc cgcctttctg cgtttcccca cgggacaagg ggaactgtcg
    62581 gtcattgttt ctcgctgcgc cgaaaggagg cccacatgga agttcccgta ccaaccaagg
    62641 aggaatacac ttatcaagtc ggaaaaatcc aatttattgt cacgcccgtc tacaaggaca
    62701 aaggcgagtc tatgcgtgac attctcttga aactgatgtt ggctgatttg gagccagctt
    62761 aacttttcat ccacatcctt aacacgggcg gtcaacggag gtataatata aataggataa
    62821 aacctcttgt ttgactgctg gaaggaggat attttgaaac agtcaagcaa gaaaaccgcc
    62881 ctcggcacag ccgcccttta ctgccgactg agccgtgatg ataatatgga caacgagtcc
    62941 aacagcatca gcaatcagaa aaagatactg caaaaagccg ccaaggacaa gggctatacg
    63001 gacaccatct ttttcgtgga tgacggtatc accggcacca ccatgaagcg ccccggcttt
    63061 cagaaaatga tcgccgcaat cgaggccggg tatatctcgg cggtgttcgt caaagacctg
    63121 tcccggctgg gccgcaacta cattgaggtg ggtaagctca ccgaggagtt tttcccgctt
    63181 catgatgtgc ggctggtggc cgtgtccgat ggcgtggaca gcgacgaggg cgaggatgac
    63241 ttcaccccgt tcaaaaacat aatgaatgag tactacgcca aggacatctc caagaagcgc
    63301 cggatcgtca ataagatgaa aggcaacgcc gggattccgc tgtccccacc gccctatggc
    63361 tatatcaaaa acccggacga tccccggttt tgggtggtcg atcccgtggc cgccgatgtg
    63421 gtgcgccgta tctaccgcat ggcgctggag gggtacgggc tggccgaaac tgccgccgct
    63481 ctcggcgctg atggtatcgt caaccccacc tactactggc gcagcaaggg tacaagccgg
    63541 ggcggttcca aaagtacctt ggagcctacc aagtggggcc ataccacgat caagaaaatc
    63601 cttaccacgc aggagtattg cggcgatgtc atcaacttca aaagctactc caaatcctac
    63661 aagatgaagc gccggatcga aaacccggag gaaaaccggg cgatcttcct caatgttcac
    63721 gaggctatta ttgatcggcc cacttgggaa aaggtacagg cgctgaaggc cggaacccgg
    63781 cgcaagcgcc ccaccgtcac ccaagagcca agcgcctttt ccggcgtgat gaagtgtccc
    63841 gagtgcggcg gcaatctgaa cttccacttt aatcagaaca accacgacat caagtttttc
    63901 agttgccaaa accataattc ggggctgcgt aaatgctcgt ccacccacta tattcggctg
    63961 gacttcttgg agcaggtcgt tctctacgag gtgcatcggc tggcctgttt cgccaacgag
    64021 tacgaaaacg actttatcaa agcgatggtg ggccgctcgg caaaggtagc cgaaaatgag
    64081 cgggtacgca aaaagcggga gctggatggg ctgctggccc gtgaccggga gttggacaca
    64141 ctctttgagc ggctgtatga ggacaatgtt tccggcaaga tcgacgatgc ccggtttgcg
    64201 aaaatggcaa agcgctatga gcaggagcag ggcgagaacg ccggacgcat caaggccctg
    64261 cggctggaag tgaaaaagct ggaagaaaag cggatggatg tagatgactt cttggaaacg
    64321 gtacgccact acaccaacgc cgccaagatc accaaacgaa tggtagccga gctgattgac
    64381 cacatcgagg tctatcctgc ggtcaaggag gacggtgtaa ccaaccagcg ggtgacgatc
    64441 cactacaact gtatcggggc ctttgaggtg ccggatcgcc gcaaaatccc cgagcgggac
    64501 atcctgctgg aaacgagaaa aggcgtagca ttaagctacg ccccggcggt gtaaaggtgg
    64561 tgtgggatat gagagttttc atttactgcc gtgtggcccg tgacgatacc tttgcattgg
    64621 aacaccagcg gttcttgctc cagctttacg caaagcgggc gggatatacc attatcggcg
    64681 ctgcggacga atacggcagc gggttgaccc ttgaccgtcc gggcatggga aaggtcacac
    64741 aggccgttct cgctggcgag gtggatgtgg tgttagtcca tagtctgacc cggataggcc
    64801 gggaatgggg gatgacccaa agctatatcg accagctcac ccggcacaag gtcaagctcc
    64861 tttgtatcaa agaacgctgc ttgttcagcg aaaaaggcgt gatccatttt tgagcataaa
    64921 aatggacgtt atgctgaaat gtggaaacat tatacagagg cgattgattt gaaagttcaa
    64981 acaaaggtgg tgcagtaaaa tgaaaaaatt aaaagaaata ttatttttat cagataaagg
    65041 ctatactgat ttgaaaaaag caatcattgc ttgtacgatt acaaatttag caatgttact
    65101 tccgttttgt attacagtta tggtttttag tgagctatta aatccttttt tagggcaaga
    65161 gttagattgg caaaatatct ggttgctttg gggagcaggt attatttcag caatcattgt
    65221 tttcttagct tccaaaaatg attatcgcaa gacttatatt gcttcttata aagagtcaaa
    65281 tgttactaga ttgcgtattg ctgaacatct gcgtaaactt cctatgagtt tcttcaacac
    65341 aaaggattta tcagaactta caacaaatat gatgtcggat tgtagtagta tggagtcaat
    65401 gttaagtagt actattccgc ctctaattgc aaatggaatt tctgtaactt taacctgtgt
    65461 tcttcttgca ctatttgatt ggcgtttagc attatgcgtt ttcattacat taccgattgc
    65521 tttcttagtt atttggtgta gcaggaaata tcagataaaa ttatttgaaa agcaagtgga
    65581 aacaaaatta aatgcgtcca gtcaagtaca agaatattta gagggaataa aaatcattaa
    65641 atcatgtaat ttaagcggcg ttcactttag gactttgaac agagcgttat tggaaatgaa
    65701 aaaaattgct gttaaagttg aaatggttgt cggtattttt atgtccagtg caagcatgat
    65761 tttacaagca ggaataggaa taactatatt tgtagggaca atcttattag tcaatggaca
    65821 aattgagcta ttacctcttc ttatgttttt cttgattgta acaaagatat atggaccgat
    65881 attagctatt ttatctcaat taactacatt attaaatctt aatgtcgtta cagaaagaat
    65941 gaaaacatta ttgactactc ctgctatgag tgggaaagaa gaaacacctc aaacttataa
    66001 tattaagtta aatgatgtat cttttgctta taacaatgaa gatgttattc acaatgtttc
    66061 atgcacaata ccacagggaa gcataacagc gttagttggt ccgtctggaa gcggaaaaag
    66121 tacaatcgca aaattgattg ccagattttg ggatatacaa agcggttcaa taacgattgg
    66181 taataaaaac attaaaacaa tcaatcctga aaatttaatg gaaaagatgt cctttgtctt
    66241 tcaagatgtt acattgttta atgatacagt ttttaataat attcaaatgg gaaatcctaa
    66301 tgctacaaaa gaacaagtat ataaagctgc caaagtagct tactgtgatg aatttgttcg
    66361 taatctgcca aatggatatg atactatttt aggagaaaac ggaagtacat tgtcaggtgg
    66421 agaacgtcaa agaatttcta ttgcaagagc tttattaaaa gactctccga ttattctttt
    66481 agacgaggca accgcctctc ttgacccaga aaatgaagtt ttcgtacaaa aagcacttgc
    66541 acatctgata aaaggtaaaa cagttattat gattgcacac cgtctaagga ctgtggtaga
    66601 tgcagaccag atattagtaa tggaaaatgg aaaaatagtt gaacatggaa cacataatga
    66661 gcttatggaa agaaaagggc tatatcacaa attatattct attcaacaag aaagtctggg
    66721 gtggtctgta taatcgtata gaaaaactga tatagaatag aaaaatagta ttataagagc
    66781 agaaacatta aattttcaat gatttacaat atcataactt gcttttcaag atgtttatgc
    66841 ttaaaggaga aacacacata tgattggtct gaaaaaacga gatattaaaa aagcattgaa
    66901 agctggggca aaagctggcg gtgtatcatt gtctgatgtt cgggcggata ttgaagcaac
    66961 gattgatgaa gctatgaaca gcaccgaccc agaggtgcag gcaaatttta aaaagtattt
    67021 tggaaataaa cgccctacat cagaagaata tatttacaag attacccaaa agacaaaagt
    67081 gtaatacaaa acaagtgctg taagggggat tgtattatgg aacattttaa gcgaaattct
    67141 atacaatcat acactaccta atagcatttt attaaaaact gaatatttta cagctttaat
    67201 agctcgtata aaactatatg ttttgtgcgg gctttttttg tacccgaaaa aattttttca
    67261 atttttttca aattcctgca acaaatcaca ccttgccgta cagatatggt gcggaaagag
    67321 ggataaacac ttttcacgtc ataacggaaa gggggtgtga tgatatgaaa ccgtcgaact
    67381 tccaaagaac gatacaatgt caattcgatt gcaagctcaa acgtgtagta aagggcattg
    67441 tccgtaatta ccgcaaagaa ttaaagcgac gaagaaataa ggaaatttcc tattgtgaac
    67501 ttccagaaat cgtcgtggaa aagctggctg tctgggacga atatgaaagc gattataccg
    67561 cctttgatgt atgcggtatt gaggttcgtg tccttgatga tgatttagca gaagcgatta
    67621 agtatctgtc tgaaaaggac agagaaatct tgttgatgta cttcttctta ggcatgagcg
    67681 atacagaaat cggcgacaga ttaaagatta accgttcaac gtcgttccgc agtaggaaga
    67741 actcacttga agaaatcaag aaaaagttaa aggagaacat gaatgatgaa taacacacgc
    67801 ccgtcatttt accttatctc gtcggctgtg gacggcaacg taaattcgat tgaaaagata
    67861 ctggcatttt atgacccgta catatcaaaa tgttgtttac gtccattcta tgacagatat
    67921 ggtaatgtct gcatagtcgt agacatggaa ttaaagggac gtatcagaga agcacttatc
    67981 aaaatgatgt tggactttga tattccttta gaaaccgaag aataagaagc agtaaccgca
    68041 gagcatgatt tgttcaatcc cctctttcct gctctgctcc tgcttttaca tgaaagcaac
    68101 acgctttcgc cacagctctt tgacaactga ataaagcaga cagatacgtt tgtgagatag
    68161 cgagccacgg ggactgtacg ccacgacctt ttcaaaaaga aagcgagcga cccacgcagt
    68221 gatccgagag cgactgttgg aaaagtcgtt ttgccacgac ctatcctcac agaataatga
    68281 tacgtccgca tggtgcggtt ctgcccacaa gaatgggaat agttgagata ctaacggagc
    68341 tttccaaagc cgtctgcctg cttgtaaaaa acgacacaca gtaaaggggg tgtatagatt
    68401 atgaacaata ctgatgtgcc tatctgggaa aaatatacgc tgacgattga agaagcgtct
    68461 aaatacttcc gtattggcga aaagaaattg cgtaaattag ccgaagaaaa tcttgacgct
    68521 ggctgggtta tcgtgaacgg taatcgtatt cagattaagc gaaaacaatt tgaaaaaatc
    68581 atagatacat tggacgaaat ctaatgtcat tgagccgtag atatggtata ataaggtata
    68641 gcgtatcacg gctcattcca tgacggaaag gagctttaca ctatgtctaa tgtgaaacga
    68701 aaagacagta aaaatcgcaa tttgcgtaat ggagagagcc agcgaaaaga cggaagatac
    68761 gtttataaat ataccgatat atacggaaag ccacaattta tctattcttg gaaacttgta
    68821 ccgacagaca agacacctgc tggaaagcgt gatgatatat cgttgaggga aaaagaagca
    68881 cagataaaaa aagaccttaa cgacggtatc gacacagtcg gcggtaaaat gacagtctgc
    68941 cagctctacg acaagaagaa cagccaaaga aagaacatca aaagggctac tgaaaaagga
    69001 cgacagtatc ttatgaacgc tctgaaaaat gacccattgg gtatgagggc gattgatacg
    69061 gttaagcagt cggacgctaa agaatgggct attagaatga gtgaaaaagg atatgcctat
    69121 aaaacgattg ataactataa gcgttccttg aaagcgtcat tttacatggc gatacaagac
    69181 gactgtatca gaaagaaccc atttgaattt aagctaagtg atgttctgga agatgatacc
    69241 gaacagaaag ttatccttac accagagcag gaagaacgcc tgcttgcctt tatggaaaag
    69301 gacaaaattt atagtaagta ttatgatgag gttgtgcttc tgctggaaac gggacttcgt
    69361 atttctgaat tttgcggact gacgacgcat attgatatgc agaacagagt actcaatata
    69421 gaccaccagt tattgaaaga tagcgaaatg ggttactata ttgaaacgcc aaagaccaaa
    69481 aacggaaaac gggaacttcc attgacagaa cgggcttatc aagcaatcca aagaatacta
    69541 aagaacagag gaaaggcaca accgctgatt gtaggtggtt acagcaattt cctattccta
    69601 aatcgtgagg gcttgcctaa agttgcagga aactatgagg gcatggtgcg aggactgatt
    69661 aagaagtata acaagtatca cacggacaag ttaccgaaca tcacgccaca ttcattcaga
    69721 catacttatt gtacgaatat ggcaaacaga ggaatgaacc caaacactct gcaatacctc
    69781 atgggacacg ctaacataac catgacactt ggctattacg cacacggtac atttcaatct
    69841 gcaaaagcag aactggaaag actggcttgt taatatcgga gcttatattt actactcatt
    69901 tactactttt ggttgcattt tcatgctggt aaatgccagc ttatgcaagg tatcttccga
    69961 aaagaaaata ccgataaagc cctaaaatac gggatatatc ggcatttacc aacttatgaa
    70021 aagataatca gaaatttagt aagtttttat accaataaaa actatctttt ttgtaacgtt
    70081 ctttgcttcc atttccattg ttatctatat ctacatatat aaatccatat cttttttcca
    70141 tttcttgaga tgaacaactt actatatcta ttggcgacca catacaataa gctaatattt
    70201 cggttccatc agctatagct tcacctattt gttctatatg ttttcctaaa taatctattc
    70261 tataactatc atgaactttt ccatcttcaa atttatcatg cattccaaat ccattttcag
    70321 ctatcattat tggcttttga tatctatcat aatattgaga taaagtattg tataatcctt
    70381 tagggtcaat tgcccacccc caaggatttg cttttaagta ttgatttttt acattatcaa
    70441 ttggattcat ggtattttta cttgctgata ctgttgtaga gtaatagtat gatagagcta
    70501 gaaaatccat agtattctct tttaacactt tatcatcacc ttcttccata actatattta
    70561 tgttattttc tttgaagaat ctcttaatat aagctggata ctcccctcta aaacttacat
    70621 cagtaaaaaa atattgtatt ctatttcttt ttaaagcaaa tactacattt tcagggtcgc
    70681 aatcttctgg ataagatgta caatctgcca tcatcatccc tatcatcata tctgaattta
    70741 tttcttttcc aagcttctta gttaaagctg tagccatgaa ttgatgatga atagcttgat
    70801 atttggcctc ttctacatca tctacctggt ctacacaaaa tgcaactgaa ttaaatgatt
    70861 catgatacat taaatttatt tggtttataa ctatccaata tttaacttta tctttgtatc
    70921 tatttaatat taattctgca tatctaacaa agaaatccac tacttttcta ttagaccatc
    70981 ctttatattt taatactaaa ttcaaaggag tctcataatg gagtagtgtt acaataggtt
    71041 ccataccatt atctattact tcatttataa gattatcata aaactttaat ccctcttcat
    71101 taggctctaa atcatctcca tttggaaata ttctagccca atttattgaa aatctaaaac
    71161 actttaaccc tagttctttc aaaagcttta aatcttcttt gtatgtatga taaaagtcta
    71221 ttccattacg cttaggataa tacccattat tatctttcat agcaaacttt attttatcta
    71281 cagtcatatc agactcgtaa tgtttagata tatctctatc tttatcatac atatgtacat
    71341 cagctaagca ccaccctttg ccaccttcat tccaagcacc ttctaattga ttagctgcta
    71401 cagctccacc ccacaaaaaa tctttaggaa acttactcaa tttaatcact ccttatttta
    71461 tttatttaaa tcatttacta ttatttacct tatattctat agactttact attcctacta
    71521 aagacatagt tatatttaac tcgctgaata ttgtcataag agtatcttga gcatgtgcaa
    71581 ataaaagtga atgtgctctt ttttctccac tagcttcccc ttgtataatg ttagtttgag
    71641 ctctatgtgc ttctgtcata aactcttttg ctttagctag ttttaaatca gcatttttat
    71701 aatcaaacct ttctaaatca ttaagagcat gtttaatttc tgttctagcc tcacctgcac
    71761 gaagtattat ttgcattgct acttctacta attcttcatc tattacatgt tcttcagttg
    71821 tcatatttat aatttccttt caacatctaa atttaagctt cttgactctc taattttagt
    71881 tcttcttttt cataagcttt aaaaaatgga taccatatca tgaatagcaa tactatattt
    71941 attgctaaaa caataagccc tctaacatct tgatttacca taaaagtaga tataccaaaa
    72001 ggaagatacc ataaattaaa caccttactc ggaattgtca caaatcccat actcattgat
    72061 aagtaagtag ttattgatat tactactgtt cctatccaca taggtaacat taaaaatggg
    72121 ttccatgcta ctggagctcc aaacattact ggttcattta tgttcattat agaaggaacg
    72181 atacaggctc ttccgattgc ttttattctt ccagctctag ataaacacat cattagcact
    72241 agtggtaatg ttgctcccca cccacctaaa gttatccaac ctgtttgaac aacttcataa
    72301 gtatatatat tagttgcaag ccctcctgat gctacagctg ctgcattagc tgcaattgca
    72361 ctaagctgaa ttgcataagt tattggggat aatacccatc cacttatacc taaagaatat
    72421 aaaaatacta ctaagaagtt taatagtaca aatccaggca atgtttgtgc tatatctgat
    72481 actggtttaa atatcctaac tattatttca aacatatcaa agttaagtcc ataaattagt
    72541 ccccaaccta taaacattat tactgctata ggtaacatac tatcaaacca cttacttaca
    72601 aaatcaggaa gtgctgaatc ttcaccaaaa aatgagaaag acccaaataa agacattatt
    72661 aatccggaga ataatcctgc aactagtgct acaagcattc cttctcctcc aaatctattg
    72721 aagttaaata tagctccttc attcgtaatc tctggacgaa tcatcattaa cattaatcca
    72781 gcacctgcaa gaccagctac tacactcata ctttcaaatt tcttattctt cattatttcc
    72841 ataggtaata aaaatgctat aaatagtcct aaaagtccaa atgtatattg atttattgga
    72901 cttaaatcag gtaagaaact tatgtaattc ttcaagacag aaactatagt tactaaagaa
    72961 cctacaaata ccataggcaa aactttcatt atagttgatt gaactgattg tacccatata
    73021 ttcttagtta tcttatttac aacaggagta aacttttcac ttaacaaatc tataaattta
    73081 ttcataaaca atctccccat agatatttta tttgtttaat attaattcta atgcttcttc
    73141 taaagccttt acaccatcta atcttccata tgctgtttta gatatacatg ctactggaac
    73201 attatattca tcagcttttt tttgtatttc atctttcata taatctaaat gtggtcctaa
    73261 taataaacaa tctatttctg gtaaatattt atctatctct gactcacttc tagcttttat
    73321 atctgcttct atgccttttt tctttgcact ctttctcata ctagttgcca taaaacctgt
    73381 acttgctcct gagccacatg ccaacaacac ttttatattc ttcataacaa tacctccagt
    73441 atttttattc attaatttat ttcttaatga atttttattt gatttgattg tatttatatt
    73501 gtacttccta aataatttcc tttcacataa atatatttcc taataatagg aaatcattta
    73561 ctcttaacca gacacataaa tatatagtat aatgtacatt ataatacgta cattcaatta
    73621 tataaatatc atatcataca gattttattt taaaactatg agagggaatc taatgaaaac
    73681 atctaaaaaa gagttatttt taaacttcat aaaaacgcaa catgattgga tagat
//
LOCUS       DAEPLF010000003        59851 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEPLF010000003 DAEPLF010000000
VERSION     DAEPLF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59851
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.003"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(172..432)
                     /locus_tag="KR314_000168"
     CDS             complement(172..432)
                     /locus_tag="KR314_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBH0326578.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMGKFQDLVKITSKK"
     gene            649..1932
                     /gene="hflX"
                     /locus_tag="KR314_000169"
     CDS             649..1932
                     /gene="hflX"
                     /locus_tag="KR314_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBH0326579.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTSESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGENGITLDVNFDEEDFNIYREYILEK"
     gene            1940..2617
                     /locus_tag="KR314_000170"
     CDS             1940..2617
                     /locus_tag="KR314_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBH0326580.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEAGEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            2703..3146
                     /locus_tag="KR314_000171"
     CDS             2703..3146
                     /locus_tag="KR314_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH0326581.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTNCVLAPEFQE
                     GGFYKVKDALDMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            3452..4096
                     /locus_tag="KR314_000172"
     CDS             3452..4096
                     /locus_tag="KR314_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBH0326582.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVVVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITNGTGKIILGEEFYLSEQNGEILI"
     gene            complement(4182..4559)
                     /locus_tag="KR314_000173"
     CDS             complement(4182..4559)
                     /locus_tag="KR314_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBH0326583.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(4559..4945)
                     /locus_tag="KR314_000174"
     CDS             complement(4559..4945)
                     /locus_tag="KR314_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBH0326584.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            5124..5879
                     /gene="nadE"
                     /locus_tag="KR314_000175"
     CDS             5124..5879
                     /gene="nadE"
                     /locus_tag="KR314_000175"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBH0326585.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYRAEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            5962..6681
                     /locus_tag="KR314_000176"
     CDS             5962..6681
                     /locus_tag="KR314_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0326586.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(6849..7853)
                     /locus_tag="KR314_000177"
     CDS             complement(6849..7853)
                     /locus_tag="KR314_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBH0326587.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            8119..9015
                     /locus_tag="KR314_000178"
     CDS             8119..9015
                     /locus_tag="KR314_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBH0326588.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            9153..10415
                     /locus_tag="KR314_000179"
     CDS             9153..10415
                     /locus_tag="KR314_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326589.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            10491..11684
                     /locus_tag="KR314_000180"
     CDS             10491..11684
                     /locus_tag="KR314_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBH0326590.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LKRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            12313..13107
                     /locus_tag="KR314_000181"
     CDS             12313..13107
                     /locus_tag="KR314_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBH0326591.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            13201..14541
                     /locus_tag="KR314_000182"
     CDS             13201..14541
                     /locus_tag="KR314_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH0326592.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            14678..15910
                     /locus_tag="KR314_000183"
     CDS             14678..15910
                     /locus_tag="KR314_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBH0326593.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            15950..17092
                     /locus_tag="KR314_000184"
     CDS             15950..17092
                     /locus_tag="KR314_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBH0326594.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGTDIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGIGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASSTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            17103..17897
                     /locus_tag="KR314_000185"
     CDS             17103..17897
                     /locus_tag="KR314_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH0326595.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFAPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            17908..18930
                     /locus_tag="KR314_000186"
     CDS             17908..18930
                     /locus_tag="KR314_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBH0326596.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELEVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEVLPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            19029..20405
                     /locus_tag="KR314_000187"
     CDS             19029..20405
                     /locus_tag="KR314_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBH0326597.1"
                     /translation="MKEQWYKDIFARILSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKDKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(20737..21081)
                     /locus_tag="KR314_000188"
     CDS             complement(20737..21081)
                     /locus_tag="KR314_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326598.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            21569..22579
                     /locus_tag="KR314_000189"
     CDS             21569..22579
                     /locus_tag="KR314_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBH0326599.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEESTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            22715..23203
                     /locus_tag="KR314_000190"
     CDS             22715..23203
                     /locus_tag="KR314_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0326600.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            23379..25307
                     /locus_tag="KR314_000191"
     CDS             23379..25307
                     /locus_tag="KR314_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0326601.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFILSNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            25482..25892
                     /locus_tag="KR314_000192"
     CDS             25482..25892
                     /locus_tag="KR314_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBH0326602.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            25914..26216
                     /locus_tag="KR314_000193"
     CDS             25914..26216
                     /locus_tag="KR314_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBH0326603.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(26432..27124)
                     /locus_tag="KR314_000194"
     CDS             complement(26432..27124)
                     /locus_tag="KR314_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBH0326604.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(27150..28337)
                     /locus_tag="KR314_000195"
     CDS             complement(27150..28337)
                     /locus_tag="KR314_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0326605.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRIPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSISYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            28673..30550
                     /locus_tag="KR314_000196"
     CDS             28673..30550
                     /locus_tag="KR314_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0326606.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKTVYDEVIKIVPQSNDVGKKNESQEKQSIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            30862..32268
                     /locus_tag="KR314_000197"
     CDS             30862..32268
                     /locus_tag="KR314_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBH0326607.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNTVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            32296..33144
                     /locus_tag="KR314_000198"
     CDS             32296..33144
                     /locus_tag="KR314_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0326608.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            33805..34497
                     /locus_tag="KR314_000199"
     CDS             33805..34497
                     /locus_tag="KR314_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0326609.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            34494..35429
                     /locus_tag="KR314_000200"
     CDS             34494..35429
                     /locus_tag="KR314_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0326610.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVDDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            35577..36500
                     /locus_tag="KR314_000201"
     CDS             35577..36500
                     /locus_tag="KR314_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0326611.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAHAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            36484..37254
                     /locus_tag="KR314_000202"
     CDS             36484..37254
                     /locus_tag="KR314_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0326612.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVTVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            37264..38046
                     /locus_tag="KR314_000203"
     CDS             37264..38046
                     /locus_tag="KR314_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0326613.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGIAVLLSLFSFLCTFILGLLFCNINISVTLIVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            38423..38959
                     /locus_tag="KR314_000204"
     CDS             38423..38959
                     /locus_tag="KR314_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBH0326614.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            38991..39380
                     /locus_tag="KR314_000205"
     CDS             38991..39380
                     /locus_tag="KR314_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBH0326615.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            39417..39803
                     /locus_tag="KR314_000206"
     CDS             39417..39803
                     /locus_tag="KR314_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBH0326616.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGSQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            39930..41372
                     /locus_tag="KR314_000207"
     CDS             39930..41372
                     /locus_tag="KR314_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBH0326617.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVENESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLIDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            41669..42484
                     /locus_tag="KR314_000208"
     CDS             41669..42484
                     /locus_tag="KR314_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0326618.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINNFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKRVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
     gene            43085..44269
                     /locus_tag="KR314_000209"
     CDS             43085..44269
                     /locus_tag="KR314_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326619.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(44646..45755)
                     /locus_tag="KR314_000210"
     CDS             complement(44646..45755)
                     /locus_tag="KR314_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0326620.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            46218..47411
                     /gene="nifV"
                     /locus_tag="KR314_000211"
     CDS             46218..47411
                     /gene="nifV"
                     /locus_tag="KR314_000211"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBH0326621.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVI
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            47436..49361
                     /locus_tag="KR314_000212"
     CDS             47436..49361
                     /locus_tag="KR314_000212"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBH0326622.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSNVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            49383..50378
                     /locus_tag="KR314_000213"
     CDS             49383..50378
                     /locus_tag="KR314_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBH0326623.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGAEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            50705..51178
                     /locus_tag="KR314_000214"
     CDS             50705..51178
                     /locus_tag="KR314_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0326624.1"
                     /translation="MVGSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            51212..51706
                     /locus_tag="KR314_000215"
     CDS             51212..51706
                     /locus_tag="KR314_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0326625.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            51803..52324
                     /locus_tag="KR314_000216"
     CDS             51803..52324
                     /locus_tag="KR314_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0326626.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMYDDNIEADKYIREELKVPSKYSVECIISIGYSDEEKKAYND
                     SDLDYKKVHFNNF"
     gene            52513..52824
                     /locus_tag="KR314_000217"
     CDS             52513..52824
                     /locus_tag="KR314_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH0326627.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            52852..53538
                     /locus_tag="KR314_000218"
     CDS             52852..53538
                     /locus_tag="KR314_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0326628.1"
                     /translation="MKYSYKKCIIDSILLMFIIQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            53770..54654
                     /locus_tag="KR314_000219"
     CDS             53770..54654
                     /locus_tag="KR314_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBH0326629.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            54753..55928
                     /locus_tag="KR314_000220"
     CDS             54753..55928
                     /locus_tag="KR314_000220"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBH0326630.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEELLKDIPTANKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            56056..56910
                     /locus_tag="KR314_000221"
     CDS             56056..56910
                     /locus_tag="KR314_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326631.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSNESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKIKDDSSC
                     LYLDIYND"
     gene            56954..58189
                     /locus_tag="KR314_000222"
     CDS             56954..58189
                     /locus_tag="KR314_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBH0326632.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREIVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKSISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYISLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNEKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            58290..59231
                     /locus_tag="KR314_000223"
     CDS             58290..59231
                     /locus_tag="KR314_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBH0326633.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(59460..59813)
                     /locus_tag="KR314_000224"
     CDS             complement(59460..59813)
                     /locus_tag="KR314_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBH0326634.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMIW
                     LEKLKEDNTYEIYSI"
BASE COUNT        22520 a         6816 c        11077 g        19438 t
ORIGIN      
        1 gttagcaagt atcttactat ttgtaatgta tagattatat aaatctttca aaataaaata
       61 agatagcaaa aacgaattat attaaaggtt atacaataaa aatagctatg taataatatt
      121 taaactaaga gtagtcgata aatatttatg catagctatt tctaatagaa attacttttt
      181 actagtgatt ttaactaaat cttgaaattt gcccataatg taattgtaat tatctttttt
      241 gtgaggtaac atgcaactac tgcaatcttt gataccttta tctgtatact taaagtttcc
      301 accacagttt tctcctaaag catacaaagg acaatagcaa aatagacagt taaactcttc
      361 tggtttatta gttttatgac aagggaaaaa ttcgcaatct ttgtgattaa aaaacttata
      421 attttcgccc atattttcct cctaattaaa taaaatttaa attttttata ctatataaga
      481 ttttggtgat aatataatta tactaaataa ttttatgaat agtcgataat atagtattat
      541 taatcatagt attaatggta taattagtca acagaaaaat aatgtaaaaa ttatgtaagt
      601 gtattttgta attagaatat gttttataac aaaaaatgag gtgattaagt gaaagaaatt
      661 caagaaaaag cacttcttgt aggtctgaat ttgactacaa tagtgaaaaa aaatgatgat
      721 atagacacta gtgaatctat ggaagaatta aaagagctaa caaaagctgc tggggcagaa
      781 gttgtaggta gcttaattca aaataaacat tctgtagatg cagcttatta tataggaaaa
      841 ggtaaagtag aagaaataag agcctatagt gactctttag atgctacttt agtaattttt
      901 aatgatgaat tatctggtgc tcaaatcaga aatatagaga atgttgttgg aagaaaagta
      961 atagatagaa ctactttaat tttagatata tttgctcaaa gagcacttag taaagaaggt
     1021 aaattacaag ttgaattagc acaattaaaa tatagattgc cacgacttta tggaatgggt
     1081 ggtgagatga gtagaacagg tgcagggatt ggtactagag gtcctggaga acaaaaacta
     1141 gaaatagata agagacatat acttaataaa gcagcagaca taaggcgtga acttaaagaa
     1201 gtaaaaaaga atagggagac acagagagtt aagcgtttaa agtcaaatat accgattgta
     1261 gcacttgttg ggtatacaaa tgcaggaaaa tctactcttt tgaacgaatt aataaagaca
     1321 cacaaggatt atgaacaaga aaaagaagtt tttgtaaaag atatgctttt tgcaactttg
     1381 gatgtaactc taagaaaggc tcttttgccg aataaaaaag aatttttagt agtagatact
     1441 gttggatttg taagtaaact tccccatgat ttagtagagg cgtttaaagc aacattggaa
     1501 gaagtacagt atgcagattt aattttacat gtaattgatg cgacaaatac tagttatgaa
     1561 ttgcaaaaaa gtacgactga aggtgtatta aaagaacttg gtgtaaatga taaaaaacat
     1621 atacttgtat ataataaagt ggataagcta gaattggata tatatcctaa aagtcaagaa
     1681 gatattgtgt atatttctgc aaaacaaggc ataaatatgg ataagctatt aaatatgata
     1741 gaaattgctt taatggaaaa tacttattca gtaagcttga tgttacctta tgagagaggt
     1801 gatatattta gtagaataaa agataaatat aatgtggaga attttgagta tggggaaaat
     1861 ggcataacgc tagatgtaaa cttcgatgaa gaagatttta atatctatag agagtacata
     1921 ctagaaaaat aggttgaata tggatataaa ttgggaaaat atagataatc ttgaagatta
     1981 ttttataacc tatcttcttt ataaagaatc taaaacagta tctcaaatta gtaaaattag
     2041 aaatatttca agtactgaag caggtgagca acttatacaa gcaaaattaa aaataaagga
     2101 aatgcaaaaa gatgattttg aggcatctaa ggatatttta gataaatttt tagaactaga
     2161 taaaattaag agacttgatt ttatggatag cttagatgac gaaaaaatgg tatactttaa
     2221 gagaaaggtt ttcaaaagaa tccttgtgga aaaaaatgct gaagatttaa ttgttttaat
     2281 ttgggcgaca ggtgagctaa aagatgatag attcttaaag ttgcttcatc agctcacaaa
     2341 ccacagacac tcagatatta gaagaataac ttattcagca atcagaaaga ttgaatcacc
     2401 aagtagtaga gaagtactgc aaaaaggtct ctacgataaa aatgcacaaa ctagacagta
     2461 ttgtgcaaag gctctttcaa aactagggga tgaaaacagt ttaaagattt tacagcagtt
     2521 gaaagacaag aataagaatt ttgaaaaaga atatgtgctt agagcttatg acgaagccat
     2581 taagtcattg gaaaatgcaa aaaatttgaa aaagtaattt tttattaaag caatttgttt
     2641 acttaatgat gtatttctag agtttaacaa ggtaataaat aaattaatgg gaggggtttc
     2701 aaatgatagt aagacgttgt gcaggtggag tagtgtttta tgctaataaa gtacttattg
     2761 taaaaaatga taggggagaa tggacacttc caaaaggaaa gatacttgga ggaggacttc
     2821 catatgagag tgctgttcaa agagttaaag tagaaactgg catagatgct aaaatgatag
     2881 atgtggctgg cgatactatg tatgaatttt tctcaagaag tagacaacaa gaagtttgta
     2941 atgcaataat gtggtatgtt atggaagctt gtaacactaa ttgtgtactt gctcctgaat
     3001 ttcaagaagg tggattttat aaagtaaagg atgctttaga tatgttatca catcataaag
     3061 agcaagcttt agtagaagta tcttataaaa agtttaagga attaaagaaa ttagtttcag
     3121 agggcgatga tgttccagca aattaattgt gaatttagag gattctgagg aagggatagc
     3181 cccttccttt tttaattgaa gcagttttaa gcgtaaatta tatagtctct ttaaattatg
     3241 atttatcaat ttagagcttt agtagtttat atttaaaatc agtaagatat atttaaaata
     3301 aaaattaaaa agtatacaat tttattatat aattgtctta tgatattgat taaagaagta
     3361 ttaaaagtcc atttattttt aaatagaata attttataga tatataattt gatgtgtata
     3421 ttagaaaatg attaaaatag gaggaaatac aatgagcaat tacaggacat tacatgaatt
     3481 tggaacagat gaaataacta ttgaaaagtc tgtttttatt ggatatgcta agcctataaa
     3541 gagtgaagaa gaagcattag agtttataaa tgaaataaag aaaaagcata aagatgcaac
     3601 tcacaatgtg tgggcttata ctgtaggtaa aaatatgaat atacaaagat atagtgatga
     3661 tggagaacca caaggaacag ctggaatacc tacattagag gttataaaaa aagaagattt
     3721 gagagatgta gtggttgttg ttactaggta ttttggagga acaaaattag gtgcaggcgg
     3781 tctagttaga gcttatacaa aaggtgctaa attgggactt gaagcaggta agataattta
     3841 taaggtaatg tatcaagaag ttaaggttaa gatagactat actcaacttg gaaaagtgca
     3901 aaatgagtta atgaatttgg ggtattttat aaaagatact gtatatgagg ataatgttga
     3961 gatagtagtg tattcaagat tagaagatgt agagaaatta tcagaaaaaa tgattgatat
     4021 aacaaatgga acaggaaaaa taattttagg agaggaattt tatttgtctg aacaaaatgg
     4081 agaaatttta atataatcta aaaggttatt agaatagtta atacctcaaa tagccaatta
     4141 gtatttttaa gtgaaaagcc attaaattga tttaatctaa atcaatttaa tggcttttta
     4201 tcggattttg cacagaatcc tttttttact tcaaataaac cgactaaaag taaaaatatc
     4261 ccaaatattt tttttaatat atctgaagac agataattag ctaatagtga accaaataca
     4321 gctcctaaaa ccccaaacac agctataggt ataaccaatt taaaaattac atttttattt
     4381 tttatatgta tagcaagtgc tataagtgtc atagggatag atgaaagtaa atttacgctt
     4441 tgtgctattt tagtgtctat gccagcaagt aaaacgagtg caggtataag gattgttcct
     4501 ccacccattc ccattcctcc aataattcct gcaaaaaatc caattagagc aaataacatt
     4561 aaaacaccat ccttaatgct gcaattatca taattattcc aaaagaaatt ctaagaaact
     4621 ttccagtaac cttatttagt aatttagctc ctataatacc accaagaata cttccaactg
     4681 caactttaat tgttaagtta acatcataag tacctttaga tacatataaa acagaacttg
     4741 ctgtcgttaa gaatattatt atagaaagag cagttgcatg agatttatgt tcttctacct
     4801 taacgatgtc atttaatatt ggaactaaaa gagtacctcc tccagaacca aaaactccat
     4861 ttataaatcc tgtaaaaact ccaattatcg tattttttaa gttaaaatca aagtattttt
     4921 tctttaaatc atcagttttt ttcataaaaa tcaccttctg aaatagtttt accaaaatag
     4981 gcaaattata tttatataaa ataagattaa atataatctt tcatgtttgt ttaattttgc
     5041 ataaaaaaaa acatattaaa acaacatatt aatatatggt ataatatatt aaaacaaagt
     5101 agcaaatttg agaggagata acaatgtcaa atataaaaat acaaatagac aaaactgtag
     5161 aatggcttat aaataaggtt aatgaggcaa atgcaaaagg attaattgtt ggtgtatctg
     5221 gaggaattga ttcggcagta gttgctaatt taattaaaaa ggcatttcca gaaaactcta
     5281 tgggagttat aatgagcata aaaagtaatc ctcaagatag agaagatgca cttaaggtta
     5341 tagagggatg tgatatagaa taccttgatt tagaccttat agaaccacaa agtgctatat
     5401 tagacatggt agtaggaaat ttaaaagata aacatttata tagagcagaa tatttaaaga
     5461 tgacagatgc gaacttaaga gcaagagtaa gaatgagtac aatttataca attgctaaca
     5521 atctaggata tttagttgtt ggtacagaca atgctgcaga aatacacaca ggatatttta
     5581 ctaaatttgg tgatggtgga gttgatattt tacctattgc aaatttaact aaaggagaag
     5641 tatatgaatg ggcaaaagag cttggtgttc atgaagattt aataaacaaa gctccttcag
     5701 caggtctttg ggaaggtcaa acagatgaag atgaaatggg aactacttac aatatgatag
     5761 atgctgtgtt agaaggtaga ttggatgaag ttcctaaaag agaccaagag ataatagaaa
     5821 gacttcatag attaagtgaa cacaagagaa aaacgcctgc acaacctcct aagttctaat
     5881 ttactattta taagatttgt gataaaattt acaagtgatg aaaattagaa taattaattg
     5941 tacgtattaa ggagtggaaa tatgggacgt ataggtaata taattaatag aaaaggtaaa
     6001 caagatgctc aaagagcaaa gatatttact aaacatgcta gagcaatagc agttgcagct
     6061 aaagaaggtg gagcagaccc agagtacaat gcagcgttaa aaacagctat agaaaaagct
     6121 aaggcagata atatgccaaa tgataatata gatagagcta tcgctaaagg tgctggagca
     6181 ggcgctggag aagactatga aactatagtt tatgaaggat atggacctgg tggagttgct
     6241 gttatagtag aaactttaac tgacaacaaa aacagaactg ctggtaacgt aagatactat
     6301 tttgacaaaa acggaggaaa cttaggaact agtggatgtg tgtcttttat gtttgataaa
     6361 aaaggacaaa tattagttgg tttaggagat ggagtttcag aagaagaatt gatggatgtt
     6421 gcattagaag caggagctga agatttcata acagaagaag atggttatga aataataact
     6481 acaccagaag atttttctag tgttcgtgat gagttaaaag ctaaaggata tgagtttata
     6541 tctgctgatg tgaagatgat acctcaaact acgactgtat tgactgaaga atctcattta
     6601 aaaatgatga ataaattagt tgatatgctt gaagaagatg atgatgttca agatatatat
     6661 cataactggg aagtagaata aatagtataa gcatattgaa atttataagc attgaatcaa
     6721 ttttaacatg tgataaattt tatagaataa gaaattacta aaatagacat acgcacctct
     6781 aacctatatt tgcataataa tatgtaagta taggtaaggg gtgtttttat atttataggt
     6841 taagaaattt aaagtaaatt ttgagctgga cttgattgtc tataaataat tgatgggtat
     6901 ataacttgtt gctgaatacc atttatacgc ccagttactg tatcaataat catcctaaga
     6961 ctacgttctg ctgtctcttc catttttaaa tctataactg tcaaaggagg aatacttctt
     7021 gccattagtc gtgaaggtcc attacttgct gcaaatattt gaatatcctc tccaattaca
     7081 attccagatt catgaaatgc attcatcata ccaagagcta caacatcatg attacaaaaa
     7141 attacttttt ttagtgaatt ttttgcaagc attttttgag caatatcata tccactatct
     7201 attttattag aacaataata tatagaatct gatagatgta ttccatgttc tttacatgtt
     7261 tttaaaaaag atttacccct taaagtcata catgtaaagg aagttgggtt gaatacaaca
     7321 gcaatatcat ttttaccatt ttgaatagca tgtaaagctg ctatacgacc agtttcctca
     7381 ttatcaatag atacacttga aaaaccaggt atttcacgat ttacaacaac tacaggaatt
     7441 ttaggtttat tttttactag atattctata tcatcagcat tgatacctgt taaaacaatt
     7501 ccatcataat aattatttgc aaaaaagtat tcatcatcac atagatgacc aactttgtaa
     7561 ggtcgtatga tgatatttat gtttaaatgt tctaaagaaa ttatgtggtt tattgcttga
     7621 agtatattgg ttgatgccaa ctcaaatccc tcatcctgcc aataaaaagc tattttaata
     7681 ttattatgtt taataggagt agagcgttta tatccaagct tatcagctga atttaatata
     7741 agctcttgtg tatgtagcgg aatcctgcgt tcatttcctt taccacttaa gacgtacgag
     7801 acagttgctg aagaaagacc acagtctttt gcaatatctt tgattgttgc catgataaaa
     7861 tcctccttaa atagttgctt aaatttgctt ataccaataa tcttaacata tattcaacta
     7921 aagaacaata ttctacaagg tgcatatttt acaggattag aataaataaa tttaaaaaaa
     7981 cttcttactt atgttgacat aatttagttt ataggattat aatatgctta aataagctta
     8041 ttattaactc aaattaagca aagttaagca ggaacatgca atgctagctt gcaatgtgac
     8101 tgtaaagggg gaaaaggtat gagtaaggta aatatatttg aagttggtcc tagagatgga
     8161 tttcaaaacc tgaaagagtt tttagaaact gataaaaagc ttaagattat tgatggtctt
     8221 attgcttcag ggattaaaca acttgaaatt agttcatttg taagcccaaa agcaatacct
     8281 cagatgaaag atgcaaaaga agtagcaact tattgtgtag aaaaatatcc tgatgtgaaa
     8341 ctttatgcat tagttccaaa tttatatggt gctaaagttg catgggagtg tggtataaga
     8401 gatatatcct atgttatatc tttgagtgaa actcataata aaaaaaatat aaatcgtacc
     8461 catgaagagt ctattgatga gcttaaaaaa ataatgaata cttatccaga catgaatata
     8521 tgtattggaa tggctactgt atttgggtgt ccttttgaag gtttaccaaa aattgaagat
     8581 gccataagat ttgctaaacg cctttgggaa aacggtatca gggagataaa tctagcagat
     8641 acaataggga ttgctgaccc taagcaagta agagaaacta ttagtgcatt agttgaagct
     8701 ttaccagagt gcagatggca ggtacatatt catgatacta gaaatatggg aatggttaat
     8761 actcttgtgg ctattgaatc tggaattact actgttcagt ctacacttgg aggattagga
     8821 ggatgcccat ttgctcctgg agcatctggc aatactgcaa ctgaagattt agtatatatg
     8881 cttgaacgta tggggtatga tacaggagtt gattttgtta aacttcttga attatcaaag
     8941 tatcagaaga gttttgtaaa tggagtatat agtggtcatc atgtaaatat aaagtctgat
     9001 agttgtaatt gttagttcta ttgattaatc gattaaaaaa atatgttgat taaaaaaata
     9061 tgctgattgg taaaatagat ttattgataa aaaagataaa ttataaaata cattatctaa
     9121 taaaacaaat aaaaaaattg agggggatta gtatggatat tatagcttta cttttatttt
     9181 taggagttat tattattgca tttgtacgta agaataatgc aggtattttg gctctagcta
     9241 ttggtgtcat agctgttaga atttttggaa tggatgataa agaactttat ggagctataa
     9301 gcaactcaat gtttactaca cttgttggta taacactact ctttgcaata gtgaactcaa
     9361 caggagcatt aaagcttgtt gcagaaaaaa ttgtagctgc ttctggtaaa cgtgtttggc
     9421 ttatacctat agcagttttt atagcaggat ttgttgttgc tggtgttgga cctggggcaa
     9481 ttccagcact tgcaattatc ccagcaatgg ctgttcctgt agccataacg gttggttata
     9541 atcctgtaat gcttgcactt ataggtgaat gtggattaat ggcaggtcgt atgactccta
     9601 ttactccaga aggtcaaatt ataacaaccg ttgcagaagg ggttggaatt gaaaatgtta
     9661 tgcctactat cttagcctgt caaactcttt ctacaattgt attttcaata gtattgttta
     9721 taattttcaa aggatataaa cttaaaaaac ctataaatgt tatagatagt aagcaaatag
     9781 aaaaatttaa tataaagcag ataatttctc taataagcat agtagttatg attgctttta
     9841 tcataatttt tgatatgaat gttgggcttg cagcttttgc agtttcagct gtacttattt
     9901 ttttcaatat tgctgatgaa ggagaatgta tcaaatcaat tccatggagc acgatagtaa
     9961 tggttcttgg agtaggtgca atgatgacaa tcgtagatgc agcaggaggt attgacttga
    10021 tgagtaatgc attatcatca ctgatgaata gtaaaacagc tactccaatt atgagtattt
    10081 cagcaggact tatgtctatg gttagttctg ctttagcagt agtttatcca actatgatgc
    10141 ctatgtgtgt tgatatagct aaggcagttg gaggagtaga cccactagca cttattgctg
    10201 cagtcggtgt tggaggttct cttgctggtg taagcccact ttctactggt ggggcattga
    10261 ttttagcagc tatgggttca agtaagaaag actttaataa ggaagaacaa aataaggttt
    10321 ttgtacagct tttcattatg agtgcagttg gcttattggt tattgcagta gtatcaatat
    10381 tatcatttaa tgcaataaca catttaatga actaattata ataactgaaa ataattcatt
    10441 aaatttaatt tttaatatta atcaatataa tataatttgg aggggaagtt atgaaaaaag
    10501 gagcattaag caatataaag gtacttgacc ttacaagagt tcttgcaggt ccatattgta
    10561 caatgatgtt ggcggatttt ggagctgatg ttataaaaat agagatgcca agaaaaggtg
    10621 atgatacaag aggttttgga ccttttgtaa atggagagag tttatactat gcaaatataa
    10681 atagaaataa aaaaggtatc actctaaacc ttaaatcaga agaaggaaaa aatatatttt
    10741 tagaaatggt aaaacatgct gatgttgtag tagaaaatta cagaccaggt gtaatggaaa
    10801 agttaggtct gggttatgat gagttggcta aagttaacaa tcaaataatt tatggagctg
    10861 tatcaggatt tggatgttat ggaccatatt cagaacgtcc aggttatgat attattgcac
    10921 aggcaatggg tggtcttatg agtataacag ggcaagaagg aggagaacca acgagagttg
    10981 gtaatgctat gggtgatgta ctaggtggaa tgaatttaac tattggtata ctcacagcac
    11041 tcaacgctag aaaaataata ggaaaaggac aaagagtaga tgtttccctc gttgattctg
    11101 ttgtatcgtc tctcgaaact ggtatacaga gatactttga ttcaggacaa cctgaactta
    11161 tgggtaatag atatgcttca gcctatcctt atgactcttt taaggcaagt gatggaagtt
    11221 ttgtaatagg ctgtggtaat gacaaacttt ttgaaaagtt atgtaaaaaa gttcttttta
    11281 gagaagacct tttaatagat gagagattta aaacaaatct taagcgttgt gaacattaca
    11341 aggctcttaa accagaaata gaaaaatggt ctgttaagca tacaataaaa gaagcagttg
    11401 acttaatatc agaggctgga gttcctgctg ctcctatcta taatcttgat tgtgttacaa
    11461 cagatgaaca tatagcaaaa gcaagagaaa tgattgtaga tattagtcat cctattatag
    11521 gaaatatgaa agtcaatgga aatccagtta agttgatgac aacaaaagct aaaattgata
    11581 tgccagcacc atctctaggg gaaagtaacg aggagattta taatggttgg cttggttttg
    11641 atagtgataa aataaaagat ttaaaagaaa atggagttat ttgattaaaa aatagctaaa
    11701 cacacttgtg actttagtca tgggaggttc agttagaata atatcaaaaa atattaaatc
    11761 aatactgtaa atcaaatata gaattgtcag aagcatttaa gatgggaaaa gctacttaga
    11821 cattgagtct ttgtggcttt tcctattttc attaagaact ttatattatc agaatagata
    11881 tgtactaaac taaagtatta acaatttaaa ttaatagaaa ataaactacc taattatgtt
    11941 gaataaagac tactttagtg aaaataaact tatagagatg ctatttaaat tattttaatg
    12001 gaaatatcaa aaatgagact gcattctcaa aaatgagact gcgtttttta agtattttct
    12061 cgaaaatgag acaaaaatat tgttttatga gttttattga agaaaaattc ttctgtgaat
    12121 tacaatttaa tcaattttaa agttgaaaat tattaaaaat ttagtgaaat ttatgtatat
    12181 ttcgtttatt ttattaaaaa taacaatttt ttactaaaaa taaagaactt caatggtgat
    12241 tggcatagta cttgctattt tatattaaca agaaatgtag aaaaaataac aatgtataat
    12301 ggggggaata tgatggaatc tgtaatggaa aatttgaaca atcttaaggt ggaggttaag
    12361 gacaaagtct gtgttattac tataaataga cctaaagctt taaatgctct taatagtgac
    12421 actcttaggg aactatcaca agttattgat gtagtatcag aaaatgaggc aatattagga
    12481 gttattatta ctggagaagg taaagttttt gtagccggtg ctgatatacg ccagatgcaa
    12541 aactataaaa gtgaggaagg cagaaaatat gcaggttatg ctcagggaat ttttgataaa
    12601 attgaagctc ttgagaaaac agtaatagca gcagttaatg gatatgcatt aggtggtgga
    12661 tgtgaacttg ctatgagttg tgatattaga attgcatcag aaaaggctat atttggacaa
    12721 cctgaagtta atttaggagt tattccatgt tttggtggaa ctcaaagatt atcaagactt
    12781 gttggaactg gaatagctaa agagttaatt tttacgggga gacaagtaaa tgcagaagaa
    12841 gctaaatcta ttgggcttat taataaagtg gttccaagtg atttactttt agaagagtct
    12901 atgaagatga tgaaccaaat agttgaaaaa gcgccaatag caataagata tgcaaaagtt
    12961 gttattaaca aagggataga tatggattta aagaatgctc ttgaactaga gaaagatatt
    13021 gcaggtttaa cttttgctac aagagataag caagaaggaa tgaatgcatt tatagaaaag
    13081 agaaagccag tttttgaaaa taagtagcta ttataactaa attggaacaa tgtagaatat
    13141 attcaaaatc taaatttcgt gaaaaaatac atatagttta attttaaaag gagttaattt
    13201 atgaatacgg gagtaattat aagtttagta gggattatca tagctctatc tcttctagtt
    13261 gtaatggtta tgaagggaat aaatattttt attatagcaa ttgtatgttc atcattagtt
    13321 gctataacag gaagcctaaa tgtgtatgat gcacttaaag tggattatat gacaggattt
    13381 gttggatttc ttcaggcaaa tttctttatt ttcctaacag ggacattaat gggaaaaata
    13441 atggagatta ctggtggagc aaaatctatt gctaagatga ttgtaagatg gattggtaag
    13501 gataaagcac tactttctat tccaatagct tgtggaatat tagcctatgg tggagtaagt
    13561 gttttcgttg taagttttgc agtgtttccg atagctttag aaatttttaa agaagctgat
    13621 ttgccacgaa gatttatacc agcagctttg acttttggat gctcaacttt tgctatggtt
    13681 gcaccagggg caccacaaat tcagaatatt gttccagcat caacattagg gacagatatt
    13741 atggccggag cagtaaatgg ttttatatca tgtgcagtaa tgtttatagt tggaagtacc
    13801 attttgtaca aaatggtttc aaaagaaaaa gccaatggtg gtcattttat agcacatgaa
    13861 tcagatatat ttgatgaagc agaagcaact aattctaaga gagaaaatgg tcctaatggt
    13921 gtaattgcct taattccatt gatagtgtca attttaataa ttaatgtaaa agttaatgga
    13981 aaagcgatag taccaattga ggtgggagta tttattggag ctttattagt atatttattg
    14041 ttaaataaat atcaagataa tactaaaata gttggacatg ttggtgatgc atgtaaaact
    14101 acagtagtag caatatgtaa tacttgtgct gtagttgcat ttggttcagt agtaaaatca
    14161 gcagttggtt ttgacttcat agttaatgcc atgactaata ttcctggtcc tcctattgca
    14221 ggggctgcag ttggaactac agttattgca ggtatttgtg ggtcagcttc aggtggacta
    14281 ggaattgcag ctccacttct tggaccagta tttttagcca agggtgtttc cgctgcagca
    14341 ttaactagga ctatggcaat ttcatcagca gcattagatt cacttccaca caatggatat
    14401 attgttactg taacaaatgg tctgtgtaaa gaaacacata aggatgccta tatgccaata
    14461 ttctgggtaa ctgttttaac tccgtttatt ggaaccatag tagcagtcct actgtttaca
    14521 atgttcccgg gattgccata agagaaaaga taaaaggtat aaattatatt ttgatattat
    14581 aaaatcaatt ttcaaaatat atacaataaa tttcatgttt taatactata aatatgtatt
    14641 taaaataaca aattgaccat ataaggagga attgaaagtg ttaaataaac cattagaggg
    14701 agttaaggtt attgacctaa cgtattttgt agcaggtcca gggacttcta aaatattagc
    14761 tgattggggt gctgacgtta ttaaggtaga gccaagcttt ggagacccag gaagaaagac
    14821 aggggcaaca atgacaatgc caatagatga ttataataat cctttttaca gcacatataa
    14881 ttcaaataaa agaggtttgt ctataaatct taagagtgaa actggaattg aaattatgga
    14941 taagctttta agtgaagcaa acgtatttgt ttcaagttat agaacaggag cattaaaaag
    15001 actaggtctt gattatgaat cattaagtaa aaagcatcca caccttatct ggggtcagat
    15061 aaatggtttt ggcgattttg gacctgctaa ggataatgca ggatttgata cagttgcctt
    15121 ttgggcaaga agtggggcta tgcttgatat agcagaaaaa gatacaagtc caataaatcc
    15181 acctatagcc tttggagatg caacaacttc ttgtagttta tctggaggaa tatgtgcagc
    15241 tttgtaccag caagctaaga ctggaaaagg acaaaaagta atggcatctt tatttgggca
    15301 agcaatatgg aatgcatcat ctttattagc atctacacag tttagtgatg agtatccaaa
    15361 aactagaaaa aatgctattt caccagtaat aaattcttat cgttgtaagg atggaaagtg
    15421 gatattctta agtattttag agcatgaaag atattttgag gcactttgta gattatttgg
    15481 aagagaagac ttaataaaag aagagaaatt tgcatcttca gtagaaagta agaaaaatgc
    15541 tacagaactt attaatattt tggatggtga atttgcaaag tttactcaaa atgagatggt
    15601 tgaaaaactt actgctgctg atatagctca tgaaaaaatt caacatgtta atgatgtagt
    15661 gactgatgaa caggctattg ctaataacta tatatatgag catacaagta aaaatggaaa
    15721 taagacaatg cttgcatcca ctccagttaa atttggaaat attgaagtta acatgacttg
    15781 tgatgctcca cttataggag aacattcaga tgagatattg agagaacttg gatacagtga
    15841 ttctgatata gaagctctaa ttgaatctgg aattgtaaca atgaataaag aagcatgtga
    15901 aatatgttaa taaacatgtg aagtatgtta ataaatagga gggaatttta tggattttag
    15961 attaacagaa gcacaattaa tgcttcaaag agtagcaaaa gaatttgcag agaatgaaat
    16021 agcaccaata gctgctgaaa cagataaaac aggtattttt ccaagggagc tttttagcaa
    16081 aatggctaaa ataggattta atggcattgg aacacctgta gagtatagtg gttctggagg
    16141 gacagatata gaaaaggtaa ttgttgttac tgagattgcc aaaaaatgtg cagcatctgc
    16201 agcaatatta tcaatacaca caatatatgc tcaagctatt ttgaaatttg gtactgagga
    16261 acaaaagaaa aaataccttc caatgatggc tgaaggtggg tgtgtaggtg ctttcgcctt
    16321 aactgaacct aatgctggtt cagatgctgc aagagctgca acaactgcaa ttattgatga
    16381 agaaacggat gaatatgttt taaatggaac taagtgtttt atttctggtg gaggtcaagc
    16441 agaatcatta ataatatttg cactgacaga cccatcaaag ggaataaagg gaatgtctgc
    16501 tatcattgtc gacaaaggta ctccaggttt ttcaatagga aaaattgaag aaaaaatggg
    16561 tattcatggt tcagaaactg cagagttaat atttgataac tgtagagttc caaagtctaa
    16621 cttattagga aaagaaggca aaggatttaa tatagctatg acttgtttag atggagcaag
    16681 aattggaatt ggagctcaag ctgtaggaat tgctgaaggg gctttagaag aaagtatcaa
    16741 atattctaaa gaaagagtcc agtttggcaa gccaatttca gcgttgcaag gtattcaatg
    16801 gtatatagca gatatggcga caatggtgga atcagcaaaa ttattagtat attatgctgc
    16861 agatttgaaa gctagaggag aaaaacacac taaagaagct gctatggcaa aatacaatgc
    16921 gtcaagtact gcaagagagg ttacaaatct agcccttcaa atccatggtg gatatggata
    16981 tatgaaggat tatccattag agagaatgta cagagatgcc aaaataacag aaatttacga
    17041 aggtacttca gaaatacaca aagttgttat ttcaagagca gtactaggat aggaggaaaa
    17101 gaatgaatat agttgtttgc ttaaagcaag ttccagatac taatgaagtt aaaataaata
    17161 aagagacagg tactctaata agagatggtg taccaagtat aatcaatcca gatgatagaa
    17221 atgctttaga agaagctcta aaaatgaaag atgaactagg agcagtgatt aaggttataa
    17281 gcatgggacc tcctcaagct aaaagtgctt taaaagaagc tttggcaatg ggagctgatg
    17341 aagcttattt aatctcagat agagcttttg gtggttcaga tacttgggct acatctacaa
    17401 ttattgctgc tgctatagaa aaggttggaa agtatgatgt aatattctgc ggaagacaag
    17461 ctatagatgg agatacagct caggttggac cagaggttgc agaattttta gggataccac
    17521 aagttactta tgcaaaagaa gtaaaggttc aagatgacaa attactagta actcgttata
    17581 cagagacagg ggattattta atagaagcaa agatgccagt tttattaact gctataaaag
    17641 agttaaacaa tccaagatat ccaagtgtaa aaggaatctt agaagcttat aataatggag
    17701 atgcgaagat tactgtatta acacttgcag atttagatgt tgatacaact caaattggat
    17761 taaagggttc acctacaaat gtttataagt cttttgcacc agttaaagac aagcataatg
    17821 aaataataga aggtataaat aaaaaagaaa aagctgagaa actaatagaa atactattcg
    17881 atttgaaact agtataggag tgttaagatg atgagagcaa aagttaatca aggtataaat
    17941 ttaaatgatt ataatggtgt ttgggtaata ggggagcaaa gggaaggcaa aattaatcct
    18001 gttacgatag aacttattgg tgaaggaaga aagcttgctg accaattagg aaaagagctt
    18061 gaagttgtga tagctggata tgaagtagag aaagaagtga aggaattact tcactatagt
    18121 gttgataaag tatattacat caatgaccct cttcttaaag atttcactac tgatggatat
    18181 gcaatttcaa ttgcaaattt aatcgaaaga aaaaagccag aggttgtatt ggttggtgct
    18241 acttctatag gaagagatat agcgcctaga attgcaggta aggttggaac aggtctaact
    18301 gctgactgta caaaacttga aatagattca acagataata agttattaca aacaagacca
    18361 gcatttggtg gaaatttaat ggccactatt gtttgtccaa aaaatagacc acagatgtca
    18421 acagttcgtc caggagttat ggcaaaagct gtaagaaacg aatcagagac tggaatttta
    18481 gaagttgtta ctccagaact tactgaaaaa atgattagaa ctagattagt agaggtactt
    18541 ccacaagaaa agaaatctgt aaatctaact gatgctagaa taatagtatc aggaggaaga
    18601 ggattaaaaa gagcagaagg atttgagctt ataaaagaat tggctgataa attgggggca
    18661 gagattggtg cttcaagagc agcagtagat tcagggtgga tagaacattc tcatcaagtg
    18721 ggacaaacag gaactactgt tagaccagaa ctatacattg catgtggaat atctggagca
    18781 atacaacact tagcaggtat gagtgattct aaatatatag ttgctataaa taaagatgca
    18841 aaagccccta tttttagtat atgtgactat ggaatagtag gagatttata tgaaattata
    18901 cctgaaatga tagaatcatt aaataggtaa cttttagaaa acattgaata tgtagaatga
    18961 taagtcttat aatattgtat tgtaagactt attatttttg ctgaaggtta tagtggggag
    19021 atgaaacaat gaaagagcag tggtataagg atatatttgc aagaatatta tctatgactg
    19081 atgatggttt tattgttgta aatacaagtg gagtaataat agatattaat gataaatatt
    19141 gtgacttttt aggaaaagaa agaaaagata taataggaca aaatatacaa agtattatac
    19201 caaatacaaa aatgctagat gttatgaaag ataagtactg tgaagaagga gctatacatc
    19261 attattcagg tggaaataca aaagaaaaaa gtgtaatagt aagtaggtct tatgtagaaa
    19321 atgacaatgg agaagtagtt gcaggagtag ctcaagttaa atttagattg caatcatttg
    19381 atgtagctaa aaaattaatg tcagaatata tggagctcca atattataaa gagcagttta
    19441 aggataactg cggttttgac aagctcatag gtgaaaatag agacttcata gaacttaaga
    19501 aaacaggtgt gaaagcttct aaaacaaact ttccagtact tttaacgggt gaaacaggca
    19561 caggtaaaga agtctttgct agagcaattc acaataatag cagtaggtca gataaaccta
    19621 tggttagtat aaactgcgct gcaataccag aagaattatt agagtcagag ctatttggtt
    19681 atgacgaagg agcttttaca ggagcaaaaa agggtggtaa aaaaggtaag tttttagttg
    19741 caaataatgg tacaatcttt ttagacgaga taggtgacat gcctttgacc atgcaagcaa
    19801 aacttttaag ggttcttcag gaaagtgaga tagaaccagt gggaggtctt aaaactataa
    19861 agatagatgt aagagtaatc tctgccacaa gaaagaattt aagtaaaatg gttgaagagg
    19921 gattatttag agaagaccta tactatagat taaatgtaat aaacattcat atgatggaat
    19981 tgaaggacag gcaagatgat attttattgc tagcaaatta tatattaaac aaattaaatg
    20041 ttgaatacaa ggaattgaag gttttaagtg ataaggttaa aaattgtttt attaactata
    20101 cttggccggg aaatataaga gaactacaaa atgtaataaa aagtgcctat gctgtaagtg
    20161 atgatatggt cattatgatg tgtgaccttc catctaagat ggataatatc agcagagtag
    20221 ctcaatgtaa tgtggatagc aattgttcaa ttcatgaaat ggttgagaat tatgaaaaaa
    20281 gcttaattat tgatgtgtta aggaagtata attggaaatg ttctaaggca gcagaggtta
    20341 tgggaataca caagagtctt ttatataaga aaataaaaaa gtatgaaatt gaattaaata
    20401 attaagataa cttgaattaa ggcaatgcta aaaaaagcgc ccttattcaa agcgaactgt
    20461 ataaactgtt ttatgcagtt aaaacgtgta aaagtagatg tgaagtacta atgtcaatca
    20521 ttagtacttt ttattgtatt tataattact atctatattt cagtatcttt tgatatctaa
    20581 gattattatg tttaatttag gatataacaa aaccctctta gttattgaca gttttttata
    20641 ttttaactgt ctactaagag gggatagttt agtttaaatt tagagtaaat tttatctagc
    20701 atttataaaa aatattttga atagcttttt tgttatttag tcataatttt ctacttcttt
    20761 atcaagttct ttgccacaag aatcacactt atatatagca ttgtatccat aatcatattc
    20821 atcaactatt ttcttttgtc taaatacacc accacactca cataggaaac caatttttat
    20881 aagtgtaacg ataaattgtg attttataaa ttttatatca ttacatttat cgttagtgta
    20941 aaatgtataa atataagagt tatcgcactc atctcttaat tcaaatcttt caatatatgt
    21001 gttatttttt aattcctcaa attcttcttt agttaaatat gatttttgtt gcaattcatg
    21061 taaacgctta atttggtcca ttatttatat cctcccaata attttagtat tttaagctta
    21121 gataaggaat ctaagtttta ttaaatttat cccaaggaat tatttaaatc atatttagtt
    21181 ttattaatta caacatatat aaattacacg aaaataaaac taattgtaaa aataatatcc
    21241 tgtgaaaacc taaactacta accccttact aacccgaaaa ctaaaaaaag aaaatatatc
    21301 caaatataag tgatatataa ataaagatgt tttatataaa tttaacttta aatattctga
    21361 caataatata tataaatata ttatagttaa aatataccac atattttggg cattgtatac
    21421 tggaattaga gtaattaatt tattttttaa catacaaatg attttatgaa aaaaaacaag
    21481 actcaaaaat ttttatattc tttgatagaa taaaattgag agtaaattta ttgaatttta
    21541 taaaatttat aaggggtgag tctaagatat gcatgaagat aaaaatgata tatttgaatc
    21601 aatagatact gaggattttg ataataaaac agaagaagaa tgtttagagc tagtaagaaa
    21661 aaatggattg aatttaatat acataaaaaa tcaaacagaa gagatatgtc tagaggctgt
    21721 tagacaaaat tgtaatgcaa taaaatgtgt tcagaatcag acagaaaaaa tatgtataga
    21781 agcagttaaa caagattgga gaatgttaga atttgtggaa gagtcaacag aagaaatttg
    21841 catgttggct attaaccaag atggaacagc attaaaatat atagaaaatc agacagagga
    21901 gttatgctta agagccgttg aaaaaaatgg tgcggctttg gagtatgtaa aagaacaaac
    21961 agaagaaata tgtatagaag cagttagaaa cagcgaattt ggattggcaa gagtgaaaaa
    22021 tcaaacagaa aaaatatgta tggaagcagt taagcattgt agctataatc taaaatgggt
    22081 agaaaatcaa acagaagaaa tatgtatgga agcagtgaga caaaatgggc ttgatttgaa
    22141 atttgtaaaa aatcaaacag aaacaatctg tttaagagca gtaagacaaa atggaatggc
    22201 attagaattt gtaaaggaac agactgttgg tatatgctta aaggcagtaa gacaatatgg
    22261 aatggcatta aaatttgtaa aagaacaaac agaagaaatt tgtacagaag ctataaaaca
    22321 agataaaaga gcacttagtt ttgtaaaagg agataaggaa aaatacaagg ctctatatga
    22381 taataatgaa ccatttgcaa agagatatgt tagaaatgta atagaaaaag aaaacagagc
    22441 attgattaaa aaaggagaag aaaatgcaaa aatcaaaatt gcaggaaaac tttatgatag
    22501 aggcatgtct tttgaagata tatcagatat agttgagatt gaaataagca agttaaaagt
    22561 gagtttaggt gttttttaaa gtaataaatg taggtgtttt ttatgggaaa tagttaataa
    22621 aaaaataatc gactagctct ttgtaggaca gcaaaattag tgatataatt tgatgtataa
    22681 taaattattt ttattagata agggggaatt aaagttgaag attacagtat tataccaaac
    22741 aagaagtgga aacacagaaa gggtcgctaa gctgatagaa gatggagcta aaaaggtgga
    22801 tggagtagat gtaaagctta tgagacttga taatattgat ttagattatt taaatgaaag
    22861 taaagcagta atatttggaa cacctactta tttagcaaat acaacttggg aagtaaaaaa
    22921 atggtttgat gaagattcaa aaaaagttaa tttagctgga aaattaggag ctgtttttgc
    22981 tacatgtgat tatatatgtg gaggtccaga tgttgcgata cttactatag taggacattt
    23041 gatggttaaa gggatgcttg tgtattctgg aggtggttct ttaggaaagc catttataca
    23101 tttaggacat gtacattcaa ttgaaggtcc agagttacaa gatgagaagg ctataatatt
    23161 tggtgaaaga attgctacta aggcaaaaga actatttgct taatttctat atgcataata
    23221 atgattagag taaatttagg gttttaatgg agatttgaag taaaagttat aaaataaaaa
    23281 attttccata aaattactca agatgaatta taatataatt ataaataaat tatattatta
    23341 aaatatttaa aatcaaacgt caatagcgag ggaagtttat ggtatggcat atagaatatg
    23401 agattttttc tgcaattata gttatttttt taatggtata cttttttaga agtaaattta
    23461 tacctacttt acaaaacaaa atatattgtg cacttttaat attctcattt ttatttatac
    23521 tttcaaatat tttaggttca ttttgtctaa ataatataga caaaattcca atttttatta
    23581 cttttttatt gaatcagata tacttattgt tattgccaat accagcagct cttgtatcat
    23641 tttatgttat ggctattatt tatcaagata taaggtatat gaaaaaaaga ttattatttt
    23701 tatctatacc acttataatt tgtataatct tatctattac aaatcctttt acgcatattc
    23761 tatttggatt gtcaaaagaa acaggttata tacggggttg tggatatgtt gcaacttttg
    23821 catctttttt cttttatgca atatattcag catttttatc ttttaaatat agaaaagcaa
    23881 tgcatgaatc aaaaatatgg gctattagag gttttcttat agtttcaatt gtggcaatta
    23941 tcttacaggc aattttttta cagtatcttc ttacaggaac tgcatgtgca tgtagtttac
    24001 ttttgacata tctttctatg cagaatcgag gtcttattat tgatgacctt actggtgtac
    24061 tcaatagaca atcttttatt caagagctag atttgaatat caatactgaa caatcaggat
    24121 ttattattac aattgcgctt gatgatttta agtttatgaa tgaaactttt ggaacaaaaa
    24181 atggagatat tgcactaaaa gaagttggaa aatatttgat tcagatacat gatgtaaacc
    24241 atgtatatag aataggggga gatatatttt ctattgttgt caatagtcaa ttaggtatag
    24301 aaccagatga tattatcaga aagatagaag aaagatttaa aaagccttgg ctagtagaag
    24361 agataagttt taatttagca actagtattg ctgttgttta ttacccagaa aatgcagaaa
    24421 cgacagaaga tgtaattgtt gctatagatt tctctataca tgaagcaaaa cgttctgagt
    24481 caaggcgagt tgtttatgca gatgcgtcta taagtgaaaa gataaagaag aaacatgtta
    24541 ttaaaaattg tttaaaagaa gcattaaaaa atgatgggtt tgaagtgtac taccaaccta
    24601 tattttctaa tagcaggggc aggtttactt cagcagaggc attacttagg ctagaacaca
    24661 aagagttagg ttttatacct cctgatgaat ttattccaat tgcagaacaa acaggattga
    24721 ttaactcaat tggattagta gtctttgaga aggtatgtag atttatagct agtgatgagt
    24781 ttgaaaattt aaaattggat aatatagctg tgaatctttc tgtagtacaa tgtatgcaaa
    24841 aaaacttagc tgatgattta ctttatctta tgaataaata taatgtatca ccaactaaat
    24901 ttaaacttga aattactgag actgtggcta tgggctcttt taatataatt aaagaaacaa
    24961 tggaaagatt aattgattta ggtgttaagt ttgcacttga tgattttgga attgcttatt
    25021 ctggagttac taatatgctt tctttaccat tttcagtaat taagttagat aagagtttga
    25081 tttggtctat gaatgaagat agtaggcata agcttacagt tgagacgatt atagctctta
    25141 taaataaatt gaatatgaag gctgtagcag aaggtgttga aaccattaag tgtgctgaag
    25201 atttaatagc tatggagtgt gaatatttgc aagggtatta tttttctaaa ccagttccag
    25261 aagatgtttt taaaaatttg ttgaaagaaa atacttttac aagttaacac cctttatttt
    25321 tttaattaca actttgttca ttgacactgc cagcgatata ttataaaatt aacacaataa
    25381 taaatattaa atttattata catattatta atattaatta atggagtaaa gatttattat
    25441 attttattta attatcctaa agtgagaaag gaagatgtga catgaataaa aagaaaatat
    25501 tagtgccaat tgatgggaca gaaagaagta tgcattcttt ggaatttata aaaggaatat
    25561 ttaaaaaaga tgaagttgaa gtagaaataa tgaatgtaaa agaactagta tttattgatg
    25621 gcatatccct ggctgaagag ataaaaaatt ctgaaaattt aggaagaaga atcttggata
    25681 aagcagcaga gataatggga gagtatgatg ttaaagtaca ttttactttt ggatatccag
    25741 gagatgagat aattagaaaa gcaaaagaag ataatgtaga ttttatagtt atgacaaaat
    25801 ctactaaaaa aggtcttaca agaatgatag gttcagtaac agcaagtgta gttaaacaag
    25861 caaaatgtat agtgatgatt gtaccggagt agaattagaa aggtatttga aatatgaata
    25921 aaaagatagt agcagtttgt gaaagtgaaa gaagtttaaa ttctcttaag acagctgcaa
    25981 atgatttagg atttgattta gtttatgaaa ttcaagaaaa tgataatata aaaaatgaac
    26041 tttctattta tgatatagaa gatgcatcta tagtgttatt tgttacaagt gattctatag
    26101 aaagcataga gaagattgat agatttatag atagagaatt ttatgaagtt gatccaaagt
    26161 atataataga ggatgcaaaa tctatagtaa gtgaaatttt aatagactta aattaatcgt
    26221 gggtgtcgca ctaagaatag agcgacgcct ttttacatat tttacataag aaataaaggg
    26281 ctgtctcaaa ctatagataa gactcttttt cattgttgaa atgatgaaaa tatatttgat
    26341 ttcatacaaa aaagagtgcc tcataactaa aaagttcact ttgagacact ccatttttat
    26401 atgttagttg catttaaggt aaaatagaag tttaatcatg ttgaaataac tgaattacat
    26461 cttctaaatg attgtacatt gatgtataac ctaagtaaga gtctttacta tcaaatgcca
    26521 tcattatctt gttatggtgg ctaatcatat ccttaagaga atttctatat ttaaaaccga
    26581 agtattggtt agaagaaact gttttaggta ttaactcata atattttata aaaattggat
    26641 tatgagaagc taaaattatc tctttgtgaa attgtaagtc agcatcataa cattcttcta
    26701 tcaaaccatg agaaacctta tccaattcct ctattgactt taggtaagta tattttattt
    26761 tttgaagttc tttagatgta gcatttaaaa ttgctagttt agtagcttca ctttcgataa
    26821 gttgtcttgc tcttaaagat tctacataaa attcttgtat gttatgattt ttatagattt
    26881 caccatcatc aaataggtca acttttttaa atccttcaac tataggactt gagtaaggca
    26941 cagctctaag tattccttcc ttacagagta tatttatggc atttcttaca ggtattctac
    27001 ttatgttaaa taggtcagca atctttctct cacttggtaa tttactatta attttaaaag
    27061 ttccatttag aatgtttaat ttaatataat caataatttt ttcagtggca tttaagcttt
    27121 tcataaaaac tcctaaacat ataatttttt tatgattgta ttatatcata gttaagagtt
    27181 aaatctactt ttttataata tgtataagac aggatagagg ccaaaatagc tataatagcc
    27241 aagataaagt atccatattg tattgaagta tattccgata ttttaccaac tatcattgga
    27301 aatacagaca ttcctatagc ataaattgct tgaaaaaaac ccattgcagt agatttctga
    27361 tgttttggta catttttcat agactcgcta gtaaggtaag aaaggagtat acctgtagat
    27421 atacctggta gcacttgtag tacaaatatt atattaatat tttttattgt tggaactaaa
    27481 aagcaatagg ctgaaactaa aataaaaact aaaggaatcc aaaattttgg cccatactta
    27541 gtacaaaatt tagtagaagc aaagccagaa gaagcaacag cacacaccat gtaagatata
    27601 gaagatagac ctatgagggt agaactagaa cctaagtttt taagaatttg agtagtaaat
    27661 gacattgttg tagacatttg aataccctgt tgaattaatg ccataaagga aaaaaataca
    27721 agtcttttat ctttgcagat agataaatat tttttaattg atattttttc aggtattttt
    27781 tttgaattat gattttttat atttaaggct agaatcgttc caataactga agacaataca
    27841 ctcatgatac ataaggctgt cattcctaat ttttcataaa ataaagtact cataacaaat
    27901 ccaatcaaca ttcctagatt gtttaccaat attatcgtac tcgtagcttt ttgttgatgt
    27961 tctgtagaaa aaaagttggt atagtgaacc atgtaagaaa tccaagttga tgatgatatt
    28021 ccagataaaa tgttagctat aagaaaacca ttgcctccag gagaaaagac tcttataaga
    28081 gatgctaaac cagcaaagaa aattccagac agtataaata ttttatgttt atttatagaa
    28141 tcagcaaata ctccaacagg aatccttaat aaacattgtg agattccata tgcacctatt
    28201 attataccaa tcatatttgt agatacatta catgcactta aatatggatt ttggaaaggt
    28261 atatatacat attgtgaaaa ccagaaaaat gtaacaatag aaagtaaaag taatttttga
    28321 ttagatattt ttttcatatt atcctccata tggtattttt ataatgcaaa taatatacca
    28381 tttagatttt ttaatcaagt actttctcta ttaaaaaaaa tttaaatata taattattat
    28441 ttacaaaaaa attatacttt atagttgaat taagaaaacg cttatgatac tataaagtta
    28501 acaaatttga atatataaca gtagacgtgt tactggtaat gcaggcaaga tcgaatcaat
    28561 ttttatagat tatctataga gtactatggg tataatctat ttatttggtt cgattttttt
    28621 gtttaattgg ccataaaaca aaaagcctat agttgaaaat ggaggatttt ttatggatta
    28681 taaaaatata gctcaagaaa ttctactaaa tgtaggagga aaagagaatg taaatgaagt
    28741 gactcattgt atgacaagac ttagatttaa agtcaaaagc gcttctaaag tgaataagga
    28801 taaattatct aaaactgaag gagtaataac tgttgttgag agtatgggtc aaatacaagt
    28861 tgtaattgga aataaagtaa aaacagtata tgatgaggtt ataaaaattg tacctcaatc
    28921 aaatgacgta ggtaaaaaga atgaaagtca agaaaagcaa agtataatta attctatatt
    28981 atcagcagta gctggtatat ttacacctac aatacctgct atagcagggg ttggtatgat
    29041 taaaggtatt ttatcagttc ttgctatgta ttatatgaat aaaaatggag tagatataaa
    29101 agaaacgcaa agttatatta tcttaaatgc catggccgat tctatattct attttatgcc
    29161 tattatacta ggatatactg cggctaaagt atttaatgca aataaaataa tatctatggt
    29221 attaggagct acactttgtt atccaacttt tactgctttg atggcaggtg aagaatctgt
    29281 tagattttta ggattggcag taacaaaagc tacgtataca tcatctgtta ttccaataat
    29341 tattgctata tgggctcttt catacgttga aaaagtatta gaaaagtata tacctgaggt
    29401 aattaaaata ataatggttc caactttatc attggttgta atgttacctg caacattatt
    29461 tttatttgga ccaatcggaa tttatatagg aaatgtcata aacttttctt ataagtatat
    29521 ttatgaatta agcccagcgt tgtgtggagc atttgtaggt gggttatggt gtgtgttggt
    29581 tatatttggt gctcatagag cattattacc aataggtata agtgatgttg ctcaaacagg
    29641 aagacagaat ttattggcat ttgcaggagc tgcaaacttt tcacaagcag gggctgctct
    29701 aggagtattc tttaaaacta aaaatcaagg actaaaaaca atttctatgt ctgcaactat
    29761 aacagcttta tttggtataa ctgaacctgc aatatacggt gccaatctta gattaaaaaa
    29821 acctatggta tgtgccgtaa tatgtggtgc atttggaggt gcgataatgg gaatgggtgg
    29881 agcttatggt aatgcatttg caaaccaagg tgttttaact atacctgtat atgctgaagc
    29941 aggtgcatta ggtttcttaa gttatctagg tggttgtgct atagcatttt ttggttcagc
    30001 tatttcaact tatctagttg gatttgaaga tttggaagag tcttctaata aggaaaattc
    30061 atttataaaa gtagaaacga aggatggtgt tattgatata acttcacctg tagagggcga
    30121 atgtatagaa ttgtcagaag taaaggatga tgtatttgct tcaaaagcta tgggagaagg
    30181 tatagctgtg ttacctacaa aaggagtaat aacagcccct actgattgtg aagtagcttc
    30241 tttatttcca acattacatg caataggatt aaaattagat aatggagcag aaatgttaat
    30301 acatgttgga atcaatacag tagagttaaa tggtaaatac tttacaaaac atgttaatca
    30361 aggagattta gtgaaaaaag gtgataaact tatatcgttt gatatagata aaataaaaaa
    30421 ggctggttat gatgtaacta cgccagttat agtaaacaat acatttgatt ttgggcaagt
    30481 agtttcttgt aaatctagtt atgtttcaac caatgataat ataatttcat tagtattagc
    30541 tggtaattaa gttaatttga ctagagataa gtaaattgat ttaaaaggaa ataagtaaat
    30601 caacaattaa atacatctaa aagtaagttt attgcaatga attgttaaag aaaataatta
    30661 atatggagta aagctattgc aatgaagtaa caaagaaaat aattaatata gagtaagttt
    30721 attgtaatga attgttaaag aaaataatta atatagggta aatctatctc aatgaaataa
    30781 caaagaaaag agttaatata gagtgaactt attgcaatga atcaacaaaa aaaataatca
    30841 atataaaaag gagaaatatt tatgaataca ggatttccaa aagacttttt atggggagca
    30901 tcatcttctg catttcaagt ggaaggagct tgggataaag acaataaagg aaaaaccgta
    30961 gccgactata attcttttaa aaagtctcat ttacaggcag atactaaagt tgcatcagac
    31021 ttttaccata attatgaaga agatattgaa ttaatgaaag aattagggat gaaaacatat
    31081 cgtttttcaa tttcatgggc aaggataata ccagatggtg aaggagagat aaaccagaaa
    31141 ggtttagact tttataataa gataatagac aaactaatag agtgtgatat tgagccattc
    31201 gtaactttat accattttga ccttccattt aaattagtag agaaatataa tggttgggaa
    31261 agtagagaaa ctgtctatgc ttttgaaaga tttgctaaaa tatgctttaa gcattttgga
    31321 gatagggtaa agtattggca acctcataat gaacagaatt taattgttag agtggaggag
    31381 agaattaata tatatgatga aacagattca tggaagatag ataaaatgcg tgctcagatg
    31441 gattataatc tttgcttagc acatgcatta gcagtcaatg catgtcatga gatgataaaa
    31501 gaaagtaaaa ttggagcagc agtatcatca tctgtaacat atcctcttac ttctaagcca
    31561 gaagatgtat atgcagctag aatgaatgat aattttaaag tgtactatat gcttgatatg
    31621 catcattatg gagaatatcc agggtattat atgaagtatc ttgaaaaaag aaatacagtt
    31681 cctcacatgg aagatgggga taaagagata cttaaaaaag ctaaaatgga ttttatagct
    31741 gtaaattatt ataggacaaa ttgtgcagaa gctttacctg aagattcaca acatccattt
    31801 ggattaagag aaggaactgt tgattttagt atgtatggac tatttaaaat gtcaatgaat
    31861 cctaacttag aggcttctga gtatggagca gcaatagacc catcaggtct tagagttgcc
    31921 ttaaatgaat attggcaaag atatcactta cctgttataa taacagaaaa tggtctagga
    31981 gctaaagata tattagaaga tggaaaaatt catgatgact atcgtataga ctatttaaga
    32041 agtcatataa atgcatgtaa actagctatt gaagatggtg tagagatgat tggatattgt
    32101 ccatggtcat ttacagacct tttaagttct tctcaaggtt ttaataagcg ttatggactt
    32161 gtttatataa atagaactga ccatgaagtt ttagatttaa aaagaatcaa gaaagatagt
    32221 ttttactggt ataaagaagt tatagagaac aatggaatag taaaataaac ataaatatat
    32281 attgagggtt ttagtatgta cattacaaag gtattaaata attcgttgct acttgcaaag
    32341 gatgataatg atgaagaaat aatattgatg gggaaaggca ttggccataa ttataaggcc
    32401 gggtctgagt taaataaaaa agatatagaa aagatttttg ttcttcatga tgaaaatata
    32461 aaaaagagta ttatacaatt ggcaacagaa atagatgcag aatattttgg tatagctcaa
    32521 atgataattg catatggaat agaaaaatat aacttaaaac ttatgaacca tatatatttg
    32581 gccttaactg accacattgc ctttgcagtg agacgcttta aagaaggtat aatgattgaa
    32641 aatcactatt tgttcgaaat caaggaattt aatccaaaag agtatgatat aggaaaatat
    32701 gctattactg tttttaagga ggttcttggt ttagacctac cagaagaaga gattggtaat
    32761 atagcaggcc attttataaa tgcacaacaa gataatcctt atagtgatag aaataagaga
    32821 agtgctaaaa ttgtaaatgc tattttgcaa atagttcatt atcacttttc tattgtatat
    32881 aataaagagt cattttatta taggagattt gtaatgcatc taaaggcctt ttcacaaaga
    32941 tttctggcta atgaaccatc aagagaaaaa atagatttta tatatgagca agttcaaaaa
    33001 aattgtaagg aagagtatga atgtgtgaaa aaggtaggag cttatatgta taaggaatac
    33061 tccagagaat taccaagaca agaggagtta tatttgatga ttcatattca taagatttta
    33121 ggagaattat gcgaaaatag ttagaatatc ttaatatgct agtaatatta taaaaaatta
    33181 aaaattaact acactttaaa tatgaacaat tgcattactt aagggggttg tcttaaaatt
    33241 gattttttta attaaatttg aagctcaact ccttttttaa acctttatat cattacaact
    33301 ttaatgttta tacgcctgta ctttagatgt atatctactt tttacatttc tcaacatgca
    33361 aacctaccat tttttgcagt atgttaattg aaataaacca ttgagttaac cctaaattta
    33421 ttctggactt ataggaatag ttccaaagat actttaaaat attttctgga ataattgctt
    33481 atttcacata tgttttattg tgatgttttg atttaataaa ttcttcaact ctgtatatag
    33541 taaatgtatt atcatgtttg ttttattcaa gcacacttat aatacgcttg attttttcac
    33601 aatgatgaaa tatactaatg ggtgttatag gtataagaat cgaattacac ttgaagaagt
    33661 aagtgataaa tgagatataa atcctcatat gataaactac tatttcattg aggaacatat
    33721 tctaagtgat gtcaagatac tacaatatga ctaaattcct agtgataaaa aagtctacga
    33781 atgagtgtag aaaggggaac taagatgaat aaagttttaa ttatagatga tgataaagat
    33841 ctttgtattt tgttaaaaaa gagtatttca attgaacaga ttgaagcaaa ctattgccat
    33901 tctgggagag atggactggt tgaactttca aaacaagatt atcagttagt agttctagac
    33961 gtgatgatgc caggtatgga tggttttgaa actatggaac aaatacgaaa atatagcagt
    34021 attcccatct taatgctgac atcaaaaagc gataattaca gcaaagtgca tggattgcga
    34081 gctggtgcag atgactatct aaccaaacca tttgaaatta cagaatttac tgctcgtgta
    34141 ctgtctttaa tccgacgtta cacccgtttt aatccaaaaa atgaggaacc acagcaactt
    34201 tctttcaaag gtttgttgat tgaccttgac cagcatacag tgactaccaa tggcgaacct
    34261 atgttgcttc cacccaagga atttggtgtg cttctttact gtgcaaggca tcagggtaag
    34321 attttgacaa aacagcagat ttatgaagct gtgtggggtg agccatatgt atatgatgac
    34381 agcaatatca tggctattat cagtcgccta agaaaaaaaa ttgaactaga cccatcatct
    34441 cccatctatt tgcaaacaat aaaaggaatt gggtatcaat tcaacacgga ggtatgaaaa
    34501 tggatgaaca aacgataatt cttttcttat tcttgctgtc gattggtcta ctttttcttt
    34561 ccatatttgc tttttccaag cttgggcata ttcacaaacg cctaaaagat atagaggaga
    34621 tactggctga tgtacaaatt ggaaacgaaa atcgcaaaat actaattaag ccatgtgatg
    34681 ttatggctcc attggtttat cagcttaatg agattgtcta cgattatgaa aataagttac
    34741 tttctctgaa aaagtcggat aaagctagca aacaacttat gacaagtcta tctcatgatg
    34801 ttcgtacacc attaacaact ctgattggtt atctggatgc tgtacacagt ggtatagtta
    34861 tcggacaaga acgggaggaa taccttgaaa tcgctaggcg tagagcctac gacctcaagg
    34921 attacattga tgttcttttt gactggtttc ggttgaactc tgatgaattt acgctgtcaa
    34981 tagaatctgt ggaaattgca gagttgtcac gcaatatttt gaaagactgg attcctatat
    35041 tccatgaaaa gaaactgaat tttgaaattg atattccaga aaatcgacta atggtaaatc
    35101 tcgacccaga tggatattcc cgtgtggtta ataatctggt tcaaaatgtt cttgctcaca
    35161 gtaaggctcg gcaaatcaag attaccatga gtgaggattc cagaatggta ttactccgtg
    35221 ttgatgatga tggtgttggg attgccagag aaaatttacc acatattttt gagcgactgt
    35281 ataagtgtga taaaggacgt tcagaaaaag gaagtggtct tggtctatct attgtcagtc
    35341 aaatggtgga gcgaatgggt ggtcagattt ccgtagaaag tgagattgga aagtataccg
    35401 tatttacagt gagcttacca ttaatataaa attgtttttc ccccatgtga catgtataca
    35461 cagggggatt ttttgtaagt tataaagttg caaggttaat acaaggttgt tgcaaggtta
    35521 atgtaagatt gaggtggtat gatagcttta ataaacaaag gaggatttta ttatttatga
    35581 ataatttaat tattgaaaca aaaaatctca ctaagcaata tggtgctcaa aagagcgttt
    35641 ctaatttgaa tattcacgtt cagaaaggtc gcatttacgg attgctagga agaaacggtg
    35701 cgggtaagac gacaactatg aaaatgttat tgggtctaac aaaaccaact actggagaaa
    35761 ttaaaatatt tggtcaggac attcacaata acgagaagaa gcttcttcct cgaattggat
    35821 gtttgattga atcaccaggg ttttatccta atctgacagg aacagaaaat ctcaaaatct
    35881 ttgcaaggct tagaggaata ccaaaaaaaa atgcagtaga atcagcactg gatgtagtcg
    35941 gtttgccaaa caatgacaaa aagttatttt ctcaatactc tcttggtatg aaacagaggt
    36001 tggcgattgc tcttgccatt atgcatgacc cacaactgtt gattttggac gagcctatca
    36061 atggacttga tcctattggt attgcagaag taagaaagtt tattcgtgat ctctgcacag
    36121 aacgaggtaa aactattttg atttccagtc acattctttc tgaaattgcc ttgttggcag
    36181 atgacattgg tattattgac catggtattt tgttggagga agaaagtctt gcccaattag
    36241 aagaaaaaaa cagtaaatac atacattttg ctgtatctga tgcagcacat gcagcaaggc
    36301 ttctggaaac ccaacttaac ctgaaaaatt tcaaagtagc agataattac aatattatgc
    36361 tatatgatac agatgtttct gttgcagaaa taaatcgtgc tttcattcta aacaacattg
    36421 aggtatcaga atctcattta tgcgaagata ctcttgaaga ttacttcaag aaagtaacag
    36481 ggggtgaagg aattgcttga tataatttca tgtgaatttt caaaactcaa gcggttaaaa
    36541 tttatactga tttctatttt aggtgcttgc ttatttccaa ttcccgcaac aattttgatt
    36601 gcaaaggaca acttgccctt tgagcaattg ttcaaattag ttgtcaattt tggatatttt
    36661 ttgctattac caattgtatt aagtattgtt gctagccaac tattttttat agaaagagat
    36721 aacgatgtat taaaaaatct tgcaacagtg ccagtgccaa aaggaaaact ggctctggca
    36781 aaattggctg tgttgctgtt tatatcattg ttctactccg ttgcagggtt aggagctact
    36841 atcattggag gtttcattgt tggaatagta gagggggtta ctgtaaaatt aggaatgagc
    36901 attgcttttg gaattatgtt ttttgttact gtacttcctg ttgtggtact cattgtatat
    36961 ttcaatcgca gctatatttt ttctattata ctttcttttg tatatgctat tttcaatttt
    37021 agtatatcct taaatattat caattttgaa ccaaataacc cactattaag cgtattacct
    37081 gctcctgtta taatgcgttg gtggatggca ttttggggag atcccactgg cgaatacacg
    37141 gcacttagac agccatattt gctctcaact cctgcttgtg taggtatttt atgtttgata
    37201 accattatag cagtcctgct gatttgtatg atatacaaaa agcaggagaa ttaggaggta
    37261 tctatgcttg atataattac tacagaattt caaaaaataa aaagatataa tattctatgg
    37321 attggtgttg ttgctgttct gttttcagca ctattggcag tttttcaggt tgtaagttca
    37381 cacggcagtg accctctaac gtatgagagc tttgcaaacg gagttatctg gaataatttc
    37441 agtcttagtt ttccatttgg gattacactt attggaggat atcttattaa ccgtgaatat
    37501 acagaccaaa ccttaaaaaa catcctaact gttcctattt cattacgcaa attgctagtg
    37561 ggaaaattaa ttgcggttgg aggaattgct gtattgttaa gtcttttcag cttcttatgc
    37621 acatttattt tggggttgtt attttgcaat atcaatatat ctgttacact cattgtaaaa
    37681 tccttagttc agattttgtc tgttaatctg tattgttatg ttgctgtttt gccgattatt
    37741 gcttattttg gtcgtaaaca aaatgcattt ctgacgggtg tagggattgc atttgtatac
    37801 gggttttgtg gtgtgtttgt tgctggaaga aatctgacag acttttatcc aatcacagca
    37861 ggtcttggtc tagttggata caacaatggt gctggtactg tttatcagcc acttatcggt
    37921 gctatgactt tgattgtaat tctaatcttg acaactattc tacttgtatt tacacctaat
    37981 tatgataaag ttatgaccat atctaagaaa aaagggataa agcataaaaa gtattctcat
    38041 aaataacata aatattgttt ctattattta agttgctaga cagtgaaaag aaaaaatctg
    38101 tcgtacagtg gctatatgct catgtattca agaagctaca gttttgattt ttcataactg
    38161 tagcttcttt ttgtatttat taaatcacgg ctatcataat acttgcaata ggtatagcga
    38221 caagagagag tactgtcatg tgtgttttat atattacatt aagtatattc taaaatatat
    38281 gttttaaata atatatttga aaataggaat atttaaataa aaaaaataat attatatata
    38341 gttgacaaaa attggcaaat gatataatat ataaatatag aaatagtaat cattactatt
    38401 attaaaaagg gaggaattaa ttatgaactt aaaaggaact aaaacagaaa agaatttaat
    38461 ggcaggattt gcaggagagt cagaagcaag aaataaatat acatactacg cttcaaaagc
    38521 aagaaaagaa ggttataatc aaatagctgc tatatttgaa gaaactgcta acaatgaaaa
    38581 ggaacatgct aaattatggt ttaaactttt acatgatggt atgccttcaa cagaggaaaa
    38641 tttaaaggat gcagcagcag gtgaaaacta tgaatggact gatatgtatg caaaatttgc
    38701 taaagaagct agagaagaag gatttgacaa gatagcttat ttatttgaag ctgttggaaa
    38761 aatagagaaa gagcatgaag aaagatactt aaagctttta gaaaacttaa atgaaggaaa
    38821 aatatttaaa agagatgaag aagttgtttg gcaatgtcaa aactgtggtc atgtgtatgt
    38881 aggtactgaa gcaccagaaa aatgcccagt atgtgaccat ccaaaagcgt atttcaatat
    38941 aaaagctgaa aattattaaa aattatcatt attaaaaagg aatgttaact atgaaatttt
    39001 ctaaacaacg agaactgatt ttaaatgaaa tattaaataa tccggttcat cctactgcgg
    39061 attacctata tgaaaactta aaaaaagata atccaaattt aagcttagga actgtttata
    39121 gaaatttagc tcagctaacg gaacatggct ttataagaaa ggttagtatt ccagggtatc
    39181 cagatagatt tgatggtcga atagataatc actatcatat catatgtgaa gtatgtggag
    39241 aggtatacga tttagaatcc gaagttctta ataatttaca agaattaata tctgaggaga
    39301 cagacataaa aataacatct tataacataa gttttaaagg gatttgtaat aattgtaaaa
    39361 ggtgtagcca agtaggttag ttttcaaatt ctaaaaataa ttaatgaggt gagtttatgt
    39421 gtagtgaaca aaaatttttt atatgtgaga cttgtggaaa ccttgtagga atgatacaaa
    39481 gtggtggtgt acctattttc tgttgtggaa aacctatgaa ggaacttgtt cctaatacaa
    39541 cagatgcagc agttgaaaaa catgtacctg taattgaagt ggatggaaat aatgttactg
    39601 ttaaagtaag tagcacaact catccaatga ctaaagaaca tcatatagca tgggtgtatc
    39661 ttatgactga acaaggtagt caacgcaaat gtctagcagt agatggagaa cctgtagtta
    39721 aatttgcttt gaatgatgat gataaagtga tttctgccta tgcttactgt aatttacatg
    39781 gattatggaa ggctgaactg taatatttaa ataaattgag tcaaagagga ggctataaat
    39841 gtattttcat acaaatagcc tcctataaat ttatataaat tcaagtttta attttgttta
    39901 atgtcaatat aatataagga gaaaataata tgtcaattta taaatgtagt gtttgtggat
    39961 atatttatga tgaatctaag aatgataaaa catgggatga attaagtgaa gattgggaat
    40021 gccctgtatg tacaaaaggt cgtagttatt ttggaaagat aagcactgtt tactatgaag
    40081 aagatgaaaa gatagcagaa gacatagttg aaaatgaatc taaattaaat actgaaaaag
    40141 agggagattt aaattactta agtacatatt taagaagaga tgatgaagtt gagaaacata
    40201 tggatataat tcacgaaatg gctgttacag gaaaatctat aatagaacct atgagaacta
    40261 aactaccagt aatttcttgg gatgatattt taattatggg tgctcaatta aatccattac
    40321 ctttaaatga gcatgatgaa gtaaatacaa caacaataat aggaaaaaaa gcaaagaaac
    40381 ctatgattat agaaaatcca gtttatattt cccacatgtc atttggagct ttgtctaaag
    40441 aactaaaaat agctttagca aagggtgcag cccaaaataa gacagctatg tgtagtggtg
    40501 aaggaggtat attaccagaa gagaaggaag cttcatacaa gtatattttt gagtatgtac
    40561 caaataaata tagtgtaact gaagaaaatc ttaaaaattc tgatgctata gagataaaaa
    40621 ttggtcaagg aacaaaacct ggtatgggag gtcatcttcc tggcgaaaaa gtaactgaag
    40681 aaatagcaaa agtaagaaat aagcctgtag gtcaagatgt aataagtcca tcttgttttg
    40741 aggaaataca atccaaagag gatttaaaga aacttataga tgaacttaga gaagtttcag
    40801 aagggcgtcc aataggtgtt aaaatttcag caggtcatat tgaaaaagat atggaattta
    40861 tagcttatgc taaacctgat tttgtaacta ttgatggtcg tggtggagct actggagcaa
    40921 gtccaaagtt gcttaaagat gcaacttcaa ttccaacaat atttgctctt tatcgtgcta
    40981 gaaaatacat agatacacat ggattagata tagatttggt tataacaggt gggcttcgta
    41041 tatctactga ttttgctaag gcaattgcta tgggggctga tgcagttgct attgcaagtt
    41101 ctgctcttat ggcagcggcg tgtcaacaat atcgtatatg tggttctggt aagtgtcctg
    41161 taggagtagc tactcaggat gaggaattga gaaaaagatt acatatagaa aatagtgcta
    41221 atagagtggc aaacttttta aatgtaagtt tagaagaact taagactttt gctagaattt
    41281 ctggtcataa agatatacat gatttaagtg ttgatgattt atatactgtt aactctgaaa
    41341 tatcaaatta tacaaacata cagcatgtat agattgaagt ttgaaactat tagattttag
    41401 atagctataa aagtaaatga taaatatatt ttataaaaga gagtgtctta aaatgaactt
    41461 tttagttcat aagtcactct tttttgtatg aagccaaata tatttatcat tttagcaatg
    41521 aaaaagaatc ttatctcttt attttgagat agactccttc tatttatgaa aattaaagat
    41581 attaaattgt aaaatattct gtgatgtcgt actattaaac taaagattct gtactttaaa
    41641 gtaaataaaa atttaaggag tgcttattat ggaaagattg aagatacaag ttttagttga
    41701 caataatact tatattgata gatactttgt tggagaacca gcagtaagct attatattga
    41761 gattgatgga aataggattt tgtttgattc gggatattca gatgtattca tatcaaacgc
    41821 tgaaaaatta aatatagatt taggaaattt aactcatgtt gttttttctc atggacataa
    41881 tgaccataca agaggtatcc agtttttaga aaataggtat gatttatcta cagttgaact
    41941 tatttcacat cctaactgtt ttatacctag aaaacatgga gagaaaagca taggagctcc
    42001 attttctgct gaggaaatta aaaatatttt tatgtataat cctaaggata agccatttaa
    42061 tctaagtaag aattgtgtgt ttttaggtga aataccttct ataaataatt ttgaaaaaag
    42121 agctaaaatt ggtaaatgta aaataggaga tttgtgggaa gatgattatg ttttagatga
    42181 ttctgctata gtttgtaaaa ctgataaagg tatttttatt gttacaggat gttctcatag
    42241 tggaatttgt aatatagtag agtatgctaa aagggtatgt ggagatgata gagttattgg
    42301 tatactaggt ggttttcatc tttttgaatt gaataatagg ttagatagta caatacaata
    42361 tttggataaa gaagaaataa gaatgttgta cccttgtcac tgtgtttctt ttaaggtaaa
    42421 agctaagatg aatgaaatat tgaatataaa tgaagttgga gtagggctga ccataagtat
    42481 atagtatttg aaagtatata agatttaagt tgaaggttaa aaagatattt tctatataaa
    42541 gtatcttttt tgttacgttt attttaagag aaatcaatat acttttatat gaaatattgt
    42601 aaaaaaattt atttttaatg agaatatatt ttcattaata aacaatttta ttatgtatta
    42661 ataatgctag aataaatata aataaatcaa agttacttac attaaagtat attttttcac
    42721 aaaatatatt gatttattct tttaaagttt gataattcca atgaatgtaa taaaagaagg
    42781 gattttatac tatacaatta ttattataaa attataaaga aaaaattaaa taataataaa
    42841 gaaaaaatgt ataatattta acattaaaaa agatatttca taaaattaaa aaacaaagtg
    42901 aatttgaata ctatttaaaa aataaaaaaa tatataaaaa atatttaata caaaggtaga
    42961 aataagttga aaatgtaaag tcattggaaa aatatagttg aatttaatga acaaaaatgt
    43021 tattgtttta tcaatgaagt gttaaaataa aaactttcaa tatcatttta gggaggatga
    43081 tttcatgcaa aaaagtgtaa agaaagcaaa agtaacggga tctatgttag ccatttttgg
    43141 tgttgcaagc gttcttttta gttcacatgc aggaggagga tttgctacag ggaatcaaga
    43201 aactcagtac tatgtgcagt atggatggac agcaccactt atggcaattt tagcaatgat
    43261 tatacttact gctaccatga gagaagttat cataatgtat aataataaca attgtaggaa
    43321 ttataaggat ttattttgtg agctttggag accatatcca aaacttgaaa taatatggga
    43381 gatatattac tatctaatgg ttttaatagc tgtaagtgct gttatagcag gtgcagcagc
    43441 agtatttcaa agtataggag taaattattt tgtagctgta tttattattg gagttgtact
    43501 tcttgtcttt actatatttg gtgctatgct tgtatctaaa gcagctacag caatgacaat
    43561 agctattcta gtatgtactc ttactatttt tattgttggt ataaaagcta aagtaccaga
    43621 aataactgaa atattatcaa atagaacgag tttcacacct ggctatatca agccaatact
    43681 taatactttt atttatgcag gttttcaatc ggttgttata cctacacttg caggatgttc
    43741 aagaccactt cttaagaact caaaagaagc tactaaggca atgataattt catttgttat
    43801 gaatgcaatt gcattaggac ttgctgtaac tatgctaatg ggatggtata gagaaattat
    43861 tgctgctggg caaacaacac tccctactct ttatgttgca ggtcaatctg gaaatcatac
    43921 tatatacata atttataatg ttgcattatt cctttgttta atgtctacag gagtaactac
    43981 tatatttggt cttgtaaata gatttgaaga ccataaagca ctttctttcc tttcttcaag
    44041 aatgaaaaga agggtattta cagcttgtgc aataatggta gtgtcaatgc ttatatcact
    44101 tacaggatta agtaatattg ttaaatatgg atatggatat tgtggttatc ttggtctatt
    44161 cactatagta attccattcc ttacactagg acattataag aataagaagt ttgcaaagga
    44221 aaatccagaa gctaaatggc cagcggaatt aaatgaaaat gtaaattaga tgtagtagca
    44281 aaaaatattg ctaaagagaa tactataaat aagaatacta tagataatat atctgcatgt
    44341 ttattaagat tatttgcaaa atctaatatt gaacaactat aaacaagcaa tataaaacta
    44401 agaataaaag ataaaaagag ggtgtctcaa aatggacttt tagtttataa ggcactcttt
    44461 tttatatgaa gtcaaatata ttttcattat tttgacaata aaagagacta atcctacttt
    44521 ggatagcctc ttttttcttt aatataaaat agtataacag caaagtatag attgatacat
    44581 gttttctgta gctttctata tattacagtg tttgtgtggc attggtattt taagcaatct
    44641 ataaattata attctataat tcttctacta attttagatt ctatagccgt ctctatttca
    44701 cttaacattc gttcattttt agggtcaata aaagcacatg tatagccttc ttcaccagct
    44761 ctaccagttc taccaatacg gtggatgtag tcttctggtg tttctggtaa atcgtaatta
    44821 tatatatgac ttactccaga tatatcaagg ccacgagaag ctacatctgt agctattaag
    44881 tattgtatat ctaaatttct aaatgatttc attattcttt ctcgttttga ttgagcaata
    44941 tcactatgaa gtttttgaca attatatcct cttatagcaa gagcttcttc aagattgtca
    45001 acccttctct tagtcctaca aaatattatt gccataaaag gattatcttc atctagtacc
    45061 ttacataagg catctaattt ttttctatct gtagtttcta ctacttcttg ttttatatta
    45121 ttaagagtga tttcttcttt ttgaatagaa actacaacag gattttttgt gtatctataa
    45181 gctaattttt ttacttgaga atttatagtt gcagaaaagc atagtgtttg ctttttgttt
    45241 gaagtctctt ttaaaatagc ttccacttcg tttttaaatc ccataagcaa catttggtct
    45301 gcttcatcta aaacaaaagt ctttaactta ctaaggtcta ttgtttttct atttagatgg
    45361 tctaaaagcc tacctggtgt agctataatc atatgaatat ttccttttag tttctttatt
    45421 tgagaaccaa tatcttttcc tccatatgct gcaagaatac ttacatcttt agattcttta
    45481 agtttcatag cttcttctgt tatttgaata gctagttctc ttgtaggaga aagtataagt
    45541 acttgtatat catttatatc taatgatata ttttcaaata tcggaagtaa aaaagctagt
    45601 gtttttcctg ttccagtttg agcttctgct attacatctt ttccttcttt tattagttta
    45661 atactttctt tttgtatagg tgtaggtgtt gttattcctg atttttttag aatatttatt
    45721 gtattttcac taatccctaa gcttttgaaa tccattgtta tcttaccttt catgaaaaat
    45781 tttctttttt attaaccaat taataatatc atatttgtaa gtatttttag taaatttttt
    45841 aattattatt tatatagctt aaaaatatat taagatactt taaaatggaa tgtaacttaa
    45901 cttaaatttt agtaggaatt ttataggttt atttttaaag gttttttgaa aataaatcta
    45961 tttaaaaatt aaattaaaat gcaagaaaaa cataataact ttcattttta agaagttttc
    46021 acaaaaaaga aaaaatatag tgcaataaat gatacaaagt aatataatag attacaagat
    46081 atgaaaagct aaaaaaataa attgcaaatg aaattaaaat gaataaaaaa taaaaaaata
    46141 atcataagtt agaggtatca atgaaaaata agacttaatg tttttaaaat taaaagtata
    46201 ctaagggggg cattaatatg tgcgttatat caaaagatag agcaaaggaa ataaaaatag
    46261 tagatactac tttaagagat ggagagcaaa cagctggggt tgtatttgca aatagagaaa
    46321 aaataatgat tgctgaaatg ttaagtgacc taggtgttga ccaaattgaa gttggtatac
    46381 ctactatggg tggagatgaa aaaaatgtaa taaagcacat atgtagtaga aatttaaaat
    46441 cagatataat ggcttggaat agagcagtaa taaaagatgt tgaagaatct atttcttgtg
    46501 gtgttgatgc agtagctata tcaatatcag tatctgacat tcatatagaa aataaattga
    46561 gaacttctag aggctgggtt ttagaaaata tggctaaaac agttgaattt gcaaagaaaa
    46621 atggattata tgtttcagta aatggagaag atgcttcaag agcagatata gacttcttaa
    46681 ctgaatttat aaatgttgga aaacaagcag gggcagatag atttagatac tgtgatacag
    46741 taggagttat gaatcctttt tctataaaga atgcaataga aactctttat gaaagaacta
    46801 attttgatat agaaatgcat acacataatg actttgggat ggcaactgca aatgcacttg
    46861 caggaatagc agcaggagca aattatgttg gtgttacagt aaatggatta ggtgaaagag
    46921 caggaaatgc agcattagaa gaagttttaa tggcacttaa gtgtgtttat aaatgtgatt
    46981 taaataatat agatactaga aaatttagag gaatatgtga gtatgtagca caggcatcag
    47041 gaagaatact tcctacatgg aaaccagtag ttggggataa tatgtttata catgaatctg
    47101 gtatacatgc agatggagct ttaaaagacc cgcataacta tgaaccattt gacccaagtg
    47161 aagtaaatct tgaaagaaaa atagtaatag gtaaacattc aggaagagct gcagttataa
    47221 ataagttgag tgaatatgaa atgtatattt ctccagaaaa cgctaccaag ctattaaatg
    47281 ctataagagc tacatctata agattaaaaa gaagtttaat ggataaagaa attcttcaat
    47341 tatattgtga tatactagca catgagaaag gcacaacaga agaagaagca gttagaggtt
    47401 catatattta agatataatt gtttaggagg aaaatatggg agataatata gtttataaaa
    47461 taattaaaaa acatatagta gatggagaag ctgttgctgg aagttcaatt ggtataaaaa
    47521 tagaccaaac tttaacacaa gattctactg gaacaatgac atatcttcaa ctagaagcta
    47581 tgggaataga taaagtaaag actaaaagaa gtgtggcatt tgtagaccat aatatgttac
    47641 aacaaggatt tgaaaatgca gacgaccata agtatataca aacagtggca gataaatatg
    47701 gagtttattt ctctaagcca ggaaatggta tttgtcatca agtattttta gaaagatttt
    47761 caacaccagg agatacactt ttaggttctg atagccacac acctactgct ggaggtgtgg
    47821 gcatgatggc tattggagct ggtggattag atgttgcttt agccatggca ggaggagcat
    47881 actatataaa agcaccaaaa gtctgtaagg tgaatctagt tggaaaatta aataatatgg
    47941 tatcatcaaa agatataata ttagaagtgt taagaaaaca aactgtaaaa ggtggagtag
    48001 gtaaagttta tgaatatggt ggagaaggtg tcaaaagcct atctgttcca caaagagcta
    48061 caataacaaa tatgggggct gaacttggag caactacttc aatcttccca agtgatgaaa
    48121 aaacactaga attttttaag agtcaaggaa gagaagacgc atggatagag ttaaaaccag
    48181 atgcagatgc agtttatgat gaagagatta ctataaactt ggatgaatta aaaccattag
    48241 ctgcaaaacc tcatagccca gacaatgtag atgaagttga aaatataggc aagattaaaa
    48301 tagaccaagt ggcaatagga agttgtacaa attcttctta tgaagactta atgaaagtag
    48361 cacaaatcct gaaaggaaat aaagtacata aggatgtgag tttggtaata gcaccaggtt
    48421 caagacaagt tatggagatg attgctagaa atggggcttt ggcagatata ataagtgctg
    48481 gagctagaat actagaaaat tcttgtggtc catgtattgg tatgggtcaa tctccaggta
    48541 ctgactctgt gtctttaaga actttcaata gaaatttcta tggtagaagt gggactttgt
    48601 cagcacaagt atatttagta agtccagaag tggcagcagt aagtgcaatc aagggagtgc
    48661 ttacagaccc aagagaattt gatattaaat ttacaaattt agatgtaaat gaatttttga
    48721 tagatgatag catgataatt aaacctgctg atgtaggaag taatgtagaa gtagttagag
    48781 gacctaatat aaaacctttc cctcttaata cagagctaag tcagtctata ggtggaaaag
    48841 ttatattaaa gacagaagat aatataacta ctgaccatat aatgccatca aatgctaagt
    48901 tacttccttt tagaagtaat ataccgtatc ttgcaaacta ttgtttcaat actgtagata
    48961 cagaatttcc tcagagagca aaagacaata atggtggatt tatagttggt ggagataatt
    49021 atggtcaagg ttcaagtaga gaacatgcag ctttagcacc attatatcta ggtgttaaag
    49081 gggtaatagt taagagcttt gcaagaatcc acaaggctaa cttgataaat agtggtataa
    49141 ttccaatgga gttttgtgat gagaaagatt atgaaaatat atctctttta gataatctgg
    49201 agataccaaa tattttagac aatctaggta gtggaatttt agaagttaag aatacaacta
    49261 aaggaacttc ttttaaggtc aaagtagaat tgtcagcaaa agaagttgac gtattaaaag
    49321 ctggtggaaa attaaactac actaaaaatc aagctaatta atatgaaaag agggaatatt
    49381 gaatgtataa agtgacactt ataccaggag atggaatagg accagaagtt gcgaaagcta
    49441 tgaaaaaggt agtagaagct actggagcag aaatagaatg ggaagaagtt aatgcaggag
    49501 aggcagttat tgaagagtat ggtactccac ttcctgaata tattatagat agtataaaaa
    49561 agaacaaaat agctataaaa ggcccaatta ctactcctgt aggtaaagga tttaggagtg
    49621 taaatgtaac tttaagacag gcattagatt tgtatgtcaa tttaagacct attaaaagtt
    49681 ttaaggggat aaaatcaaga tatgaagatg ttgatttagt ggttgtaaga gaaaatacag
    49741 aagatttata tgctggaata gaacataaaa taggtgatta tgcagctgaa agtataaaga
    49801 taataacaag aagtgcaagt gaaagaatcg ttgattttgc atgtaactat gttaaagata
    49861 ataagagaaa aaaagttaca gctatacata aggcaaatat aatgaaaatg tctgatggat
    49921 tgtttttaga tgtgtttaga gaagttgcat caaaacatgg tgtagaatat gatgatttga
    49981 ttgtagatgc agcagctatg aacttagttt taaatccaga aaattatgat gtaatggtaa
    50041 tgccaaatct ttatggagat atactatcag atttaggtgc tgggcttgtt ggaggtttag
    50101 gtataattcc aagcgcaaat attggtaaag attgtgcgat atttgaagct gttcatggaa
    50161 gtgctccaca aatagcaggt caaaataagg ctaatccaac agcacttatt caatcatcag
    50221 taatgatgct tagatactta ggtgaatatg aaaatgcaca aaaaatagaa acagctttag
    50281 aaaaagtatt tcttgaagga agtaaattga ctgtagattt aggtggctca gcttctacta
    50341 cagagtttgc ggatgaggtg tgtaagtata ttgtatagat tttatttagg gaaaaagtga
    50401 aatatagatt gaaggaaaga ggctttctca aaatagagat aagccttttt ttattggaat
    50461 gataaaatat atttgatttt atacaaaaga gagttcctca taaacgaaaa agttaatttt
    50521 gggatatcca ttttaatttt aaaattaatt gattaatacg caaagaaaga ttaaacaaat
    50581 ttattatttt taattgaact ttcaaccaaa atatggtaaa atttaaaaaa gttgaacttt
    50641 caactaaaaa taaatatatt gattatgtga aattctataa aacagaaact atttgacttt
    50701 agtcatggta ggttcagtgg aggtgtgttt gattaagact ttagatagta atatattaag
    50761 agaagtaggt actttatcta gagcagtaaa ttcaataaat gatattaagt acaaggaatt
    50821 aaagttacaa aaaggacaat ttacattttt gacaagaatc tgtgaaaatc ctggtataaa
    50881 tcttgtggaa ttatcaaata tgttaaaggt agacaaggct acaacaacca aggctataca
    50941 aaaactaata aaggcaggat atgtagataa aaagcaggat gagtttgata aaagaggata
    51001 taatttaact ccaactgata aatctttgga agtttatgag ttgataattg aagaagagaa
    51061 tagaagtata gagatatgtt ttgataattt tacagatgaa gaaaaacaag tggtaactaa
    51121 gttattagaa aaaatgagca aaaacgtaga aaatgaatgg tttaaagtaa agagatagta
    51181 aataaataca ttaacttaag gagatatttt tatgattaga aaagcgaata tgaatgattt
    51241 agaaagcatt atgaaaatta taaaatctac agtggaagaa atgaagactt ataataatac
    51301 acaatgggat gaaaactatc cattagaaaa agactttgtt tcagatatta agaaacaaga
    51361 tttatatatt tatgaagtgg atggagaagt agcaggattt atatgtctta attatgaaga
    51421 gccagaagaa tatttaggct taaattggtc atctaataaa aaagcaatgg taatacatag
    51481 aatggctgta aatccaaact ttagaaagac aggaatagca tctaagttag ttgattttgc
    51541 tgaaaaatta gctgtagaaa acaatgtatc atacttaaag agcgatacat attcaataaa
    51601 ctcaaaaatg aatttattac ttacaaagtg tggttttata aaaatcggag aaatgagttt
    51661 tttaggaaag gagaaatctt tctactgtta tgataaaata ttataaagag catttataat
    51721 acaaggggta aataatagat tttcagcatt agtaagggca taatataacc cagtatatgc
    51781 tattatgaaa gggatgattt atatgattgg atttctaaag aaaagaagaa gtattagaaa
    51841 atacaaggat gttgaagtag aaaaagaaaa attagataaa atattaaaag ctgctctttt
    51901 agctccatca tcaaaggggc ttagaacatg ggaatttata gtggtagatg ataaagaaaa
    51961 gctaattaat ttatctcaat gtagaactaa gggtggtgga tttttcttaa aaaatgcacc
    52021 tttagctatt gtaattattg cagataaaga aaaaaatgat gtttggatag aggatgcaag
    52081 tatagctgct tcatacatac aacttcaagc tcatgaacta ggtttaggtt cttgttggat
    52141 acaagtaaga aatagaatgt atgatgataa tatagaagct gataagtaca ttagagaaga
    52201 attaaaagta cctagcaagt acagtgtaga atgtataatt tctataggat attctgatga
    52261 agaaaagaaa gcatacaatg actctgattt agactataaa aaggttcatt ttaacaactt
    52321 ttagtatata atatgttgta ttaagacatt atattttttt ctattgttaa tatatactta
    52381 aaattgtaaa aatatatctg aattttacta acttatctag gggttatgta caaggatttt
    52441 aaagataaat atagaaatta atgtttataa tcgatacata acttgagtaa caaaatttaa
    52501 ggaggttaaa gtatgggtat atttaaaaca aaaatggatg aggactggaa agtaaactac
    52561 attaaagagt ttaatgaaat gagagattct tatgagagta aacttcaaaa aaaacagttt
    52621 gaggtagata gtttgaaatc agaattggat agactaagaa gctataaaaa tagccttaag
    52681 ccaaaagaaa aacaaattac tgatgatgat ataaataata taaagagttt aagaagagat
    52741 ggcttaagct ataaagaaat ttcaaatcaa acaagttgga gcaaagcaac tgtaagtagg
    52801 gtattaaatg gactttacga ttagcatata catggggtga tttaaattgg gatgaagtat
    52861 agttacaaaa aatgcataat agattctatt ttattgatgt ttataattca aatattaagg
    52921 atgattttga attatgtact tctaagtcaa tttgaattta ctcttgaaaa ttttaatatt
    52981 ataaacttaa tttcttttac attagttggt ttatcgttaa ttttattttt aaaagataat
    53041 tctttgtata acaaggttag aaatagaaaa atcacagaag cttttgaaga aaataaaaac
    53101 aatatactta ttgaaaagtg taagcttata ctttttgttg tggttttatc tttagcaata
    53161 attgtaactt attgtactaa gggatatgta ctttttaatg taactatgat gactttgtct
    53221 gtattgatag ttccaatatt tgaagagtta ttttttagag aatatatatg gaactatcta
    53281 agtaatttta taaagtcaaa gagtaaaatt atctgtataa catctatact atctggaatt
    53341 tataatatag gatacataga tgttataaga aattatgtaa tattgtataa caattcaagc
    53401 tacacctttg aagttattat ttcaaaaatt atgataggta ctgtttttgg tattgtactt
    53461 ggattagtta agtataggtt tagagatgta gggttttgta tattattgag gagcttattt
    53521 gctatattta ttaggtagat agtataatcg gtttataagg ttgttttaaa tttaatttta
    53581 aatcaacctt attttatttg tgttttataa aacacttata tattaataac taataaattt
    53641 aataaagatt atttgaatat ttttaaaata tataaatatg tattgacata taaatattat
    53701 gaattacaat ttatataaac taataaaaat taaataatat taatcaactt attgaatgga
    53761 ggaataaaaa tgaaatttgt gactttttgt agtagcaatg aagaaaaaat aggtgtattt
    53821 aattcagaaa caaatagtat atatgaaatt aatagtttag gattaagtaa attatatact
    53881 gatatgaatg attttattga aaacgtatct acaggagatt tagaaaaaat aaaaaacaat
    53941 tcatttgaaa acgcaaagtg ttataagtta gaagaagtta aattatgttc cccaattgta
    54001 agacctaaaa aagatattat atgtttaggt ctaaactata aagaccatgt caatgaaatc
    54061 ccagatggag ttataaaaaa cgtagttatg ccagattatc caatttattt ttctaagaga
    54121 gcagacaaaa taattggagt ggatgataag ataagcttac atggagattt ggtagagaag
    54181 ttggactatg aatcagaact tgcagtaatc ataggtaaag aaggtataaa tatatcaaaa
    54241 gaagatgctt atgaatatat atttggatat acaatagtaa atgatataag tgaaagagca
    54301 cttcaagata aacatgttca atggtttaga ggaaaaagct tagacacaca tacatctatg
    54361 ggaccttgta tagtacataa agaagaattt gaacatcctt taaaattaga tataagtagt
    54421 gtagtaaatg gagaagtaag acaaaattct aatacagagt actttatatt tgatatacct
    54481 actataataa atgatttatc aagaggaatg actctaaagc caggagatat aatttcaact
    54541 ggaactccag caggggttgc tatggggatg aatcctcaag tatatttaaa gcatggcgat
    54601 atagttgaat gtaaagtcga aggaatagga gttttaaaaa atatagttga ttaatggata
    54661 aatatgccat ataattttat tagtacaaat aatttaatta atataggatt gatcaaactt
    54721 atgattaatt tattatagag agggatgagt atatggcaag agaactagaa ttaaagtatg
    54781 gctgtaatcc aaatcaaaaa ccatcaaaga tttatatgaa gaatggagaa cttcctatag
    54841 aagtattaaa tggaaaacct ggatatataa attttttaga tgcatttaat agttggcaac
    54901 ttgtgaagga attaaaagaa gctacaggtc ttccagcagc aacttcattt aaacatgtaa
    54961 gtccagcagg agcagcagta ggggtacctt tatcagatac tttaaagcaa atatattttg
    55021 ttgatgattt agagctttct ccattagctt gtgcttatgc aatggcaaga ggagcagata
    55081 gaatgtcttc ttatggtgat tttatagcac tttcagatgt atgtgataag gagactgcaa
    55141 ctattatagc aagagaggtt tctgatggta taatagctcc aggatataca gatgaagccc
    55201 ttgaaatatt aaaaggaaaa cgtaaaggca actataatat tgtaaaaata gatgaaaatt
    55261 atactcctga gcctattgaa acaaaggatg tttatggaat tacttttgaa caaggtagaa
    55321 atgaaatatt aataaatgaa gaactattaa aagatattcc aactgcgaat aaaattttta
    55381 cagatagtgc aaaacgtgat ttaattatag ctcttattac attaaaatat acacaatcaa
    55441 attctgtttg ttatgctaaa gatggacaag taataggggt aggtgcaggt caacaatcac
    55501 gtattcattg tactagactt gcaggtaaca aagctgatac ttggtatctt cgtcaacatc
    55561 ctaaagtatt aaacttaaaa tttaaaaagg atataggtcg tccagataga gataacacta
    55621 ttgatgtata tctttctgat gattatatgg atgtgcttgc tgatggcata tggcaaaact
    55681 tttttgaaga aaaaccagag cctctaactg gagaaggaaa aagagcatgg cttaaaactt
    55741 taacaggagt agcacttggt tcagatgcat ttttcccatt tggagataat atagaacgtg
    55801 caaaaagaag tggggtaagt tttatagcac aaccaggtgg ttctattcgt gatgataatg
    55861 ttatattaac ttgtaataag tataacatag tcatggcatt tactaaaaat agattattcc
    55921 atcattaaaa tactgaaagt ataaagataa tatagattaa gtaaaaatct acaacaagtt
    55981 ttgttgtaga tttttttatt tgctgtataa ttttaaatag aactttattt ttagatattc
    56041 aatttggagg ttgatatggc tttaaatata agaaactata gcctattatg taatcaagga
    56101 acagctaaat ttaatgaaga tgtagtcggt ataaatccat ttggggcatg ggttttagat
    56161 ggtgctacag gacttaatgg aaaaaattta gtgtcaaatg aaagtgacgc aaggtggtat
    56221 gttcagtggt ggaataggta tttatataaa aatataaata aagaagaatc attaaagagt
    56281 attattaata ctggaataat agaagtaaaa aaagagtatg aactaagatt aaatgggttt
    56341 aaggttgaaa aattagattt accatctagt tctatagttg ttgtaaaatt tcatgaaaat
    56401 aaagtagagt attttttatt aggagattgt gctttgttta ttaaaaatgg agattccaaa
    56461 gttataaaag ataggagtat ttgtaaattt gataatactg tatttgatga aatgagtaaa
    56521 ctttctaact tggaagagat ggcatttgat gagattagaa ctagtgttat ggatacaata
    56581 gtaagcaata gattgaagaa aaatacgaaa gagggctatt ggatattgga ttttgatgag
    56641 gaagctgtgg aaaattctat atatggatac attgaaataa aaaaagattt tcaattgatg
    56701 ctaacgagtg atggttttac aagtgcatgt gatagatata atctaataaa agaagaagaa
    56761 ttgatacgta tagcagaaaa atttggtata ggatatatac ataataaagt aagggatttt
    56821 gaagataatg actataaagc ggttaaagtg ccaagattta aaataaaaga tgattcatct
    56881 tgtttatatt tagatatata taatgattag actgtataaa actatttaat tttagaaagg
    56941 aggcaagtga aaagtgaaaa ttcttcatat aataacacaa aaaccaaata gtacaggtag
    57001 tggtatatat ttgtctggta tgataaaagg gttcgaaaaa atcggtcata agcaagctgt
    57061 aattgcaggt atagatgtaa atgatgatgt aaattgtttt ccaagtgaag taagttttta
    57121 tccagtaaag tataattgtg gggagttaaa ttttcctgtt gtaggtatga gtgattctat
    57181 gccatatgaa agtacaagat ataaagattt aaatatagac atgataaata gattgaaata
    57241 tcaatttaaa gtaaatatag ataaggctat gaatgatttt aaaccagaac ttataatatg
    57301 tcatcatttg tacctcttaa ctgcatttgt tagggaaata gtaaaagata taaaggttat
    57361 gtctatttgt catggaactt gtttaagaca gttaaatacc attgatttag agaaagaata
    57421 cataatagca aatataagga aattagattt aatatttgct cttcatgaaa atcagaaata
    57481 tgatattata aagacttttg gagtaagtga aagtaaagta gttgtaattg gtagtggtta
    57541 taatgatgat atattttata ataaaaatta taaaataaag gatgataaaa taaaaatagt
    57601 atttgcaggt aagatatgta aatcaaaggg attgattcca tttataaaat ctatatctaa
    57661 attaaaatat agtaaagatt taatagaagt caattttgca ggtactggaa gcgatatcga
    57721 atcatataat gagatagtta aactggcaag taagtctcca tttaaaatga atttcctagg
    57781 aaagttagaa cagagagatt tagctgagct attcaacagg tcacaaatat ttgtgttacc
    57841 atcattttat gagggattac cagttgtagt tttagaagct ttatcttgtg gcacagatgt
    57901 aatcactact gatattcttg gtgttaaaga gtggattggt agtgagatta ataattctgg
    57961 aaagatagaa tatatttctc taccttttat ggagaaagaa ggtattccaa aagatgaaga
    58021 gttatatgat tttgaaaata atttgtataa tgcaatagat tctaagatac aatctttatt
    58081 gaataactca aatgagaaaa catctgtaga tatgtcaaaa aaaacgtggg atggcttagc
    58141 atatagaata aatgaagtta ttttaatgga tgaattgtgt ttagtctaaa attatttaaa
    58201 ttataaaaaa gtgtaactat ttttaaactt tttttaaatg attttatatt atactaatac
    58261 taaatatatt tgttttaggg aggtaattta tgaggattag taaaaaggta ttggcgttag
    58321 gtatttcagc attactgtta agtgttagtt ttccaacatc aataaacgca ctagataaga
    58381 ttgaaaatat tcaaggagta gataaatatg aaacagctgg gcttatttca gataagcaag
    58441 attttacaac agcagttctt atcaatgcag attctacaat ggtagatggg attgctgcaa
    58501 gtggtcttgc aggagtaaat aatgcagcta ttttattaac taataaggat gatataccag
    58561 aagcaacact tcaaagactg aatagagtta ctaagatata tgtaataggt ggagaaaatt
    58621 ctataagtaa agatgtagaa aaaatgttac ttatgagaag aatgcaagtt ataagaattg
    58681 atggtgtaga tagagtagat acaagttata aaattgcagg tgaaatagaa aaaattaaaa
    58741 acagtgataa gatgttttta gtaaatggat ttaaggatga agctgatgca gtaagtgttg
    58801 catctgttgc ttatagagat ggagctccta taatattgac aaaagatata ccttcagcag
    58861 aagaagacac agacttagac ccatggtttg gagtttctcc tgttccagta tatgctattg
    58921 gaggagagtc tacattaagt gattacatag tgagcagata tagagcaact agaattggcg
    58981 gtgttgatag atatcaaact aataaaaatg ttattgaaaa gttctataat ggagcaaaag
    59041 agttctatat aacaagtgga gacgatttag tttatgcttt agttgcatca ccacttgcaa
    59101 aaaatgctcc tgttgtttta gtttcaaata aaagtgataa gagtatatta agtggagcta
    59161 gtaaagtaac agcaataggt ataagtgata agagtattat agagcaatgt ttggatgctg
    59221 taaagaagta atggagtgtt attagtaaaa tgaatatata cattgaaaat tagtagattg
    59281 ttcattttat aatggtataa aatatatggg aagaggctat caaatggaga taagcctctt
    59341 ttttattgat aaaattatga aaatatactt tattttatat aaaaaaagta tctcatgaac
    59401 taaatgtcta ttttgatata ctatctcctt ttcttatatt atatgtatta tatgattttt
    59461 tatatggaat aaatttcata agtattatct tcctttaatt tttctaacca aatcatctct
    59521 aaaatatcta gttcttctaa atagtctttc tctgttttgt tatcatcatg ttttataagt
    59581 tctaaaactt caaatgtaaa tgctttttca ccatattttt tccattcatt atttaattct
    59641 tttatcatac atgagccagc accaagctga aatttgagag agttaaattt gctttttatg
    59701 tcattacttt ttccaaggta aatcttttta gtaatatcat tttttattat gaatacacct
    59761 gtgtcaaatt tcatttcttt gtataattgt tttaattcac gttttctatc catgatttta
    59821 gatacctctc tttataattg atatgtaata t
//
LOCUS       DAEPLF010000004        58729 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEPLF010000004 DAEPLF010000000
VERSION     DAEPLF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58729
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.004"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            153..1349
                     /locus_tag="KR314_000225"
     CDS             153..1349
                     /locus_tag="KR314_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBH0326635.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1442..1966
                     /locus_tag="KR314_000226"
     CDS             1442..1966
                     /locus_tag="KR314_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBH0326636.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1991..2167
                     /gene="rpmF"
                     /locus_tag="KR314_000227"
     CDS             1991..2167
                     /gene="rpmF"
                     /locus_tag="KR314_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBH0326637.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2374..2931
                     /gene="fapR"
                     /locus_tag="KR314_000228"
     CDS             2374..2931
                     /gene="fapR"
                     /locus_tag="KR314_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBH0326638.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2942..3964
                     /gene="plsX"
                     /locus_tag="KR314_000229"
     CDS             2942..3964
                     /gene="plsX"
                     /locus_tag="KR314_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBH0326639.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3990..4976
                     /locus_tag="KR314_000230"
     CDS             3990..4976
                     /locus_tag="KR314_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBH0326640.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5085..6014
                     /gene="fabK"
                     /locus_tag="KR314_000231"
     CDS             5085..6014
                     /gene="fabK"
                     /locus_tag="KR314_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBH0326641.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            6030..6980
                     /gene="fabD"
                     /locus_tag="KR314_000232"
     CDS             6030..6980
                     /gene="fabD"
                     /locus_tag="KR314_000232"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBH0326642.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIEILLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6980..7729
                     /gene="fabG"
                     /locus_tag="KR314_000233"
     CDS             6980..7729
                     /gene="fabG"
                     /locus_tag="KR314_000233"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBH0326643.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTRVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7783..8007
                     /gene="acpP"
                     /locus_tag="KR314_000234"
     CDS             7783..8007
                     /gene="acpP"
                     /locus_tag="KR314_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBH0326644.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            8036..9274
                     /gene="fabF"
                     /locus_tag="KR314_000235"
     CDS             8036..9274
                     /gene="fabF"
                     /locus_tag="KR314_000235"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBH0326645.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9500..11218
                     /locus_tag="KR314_000236"
     CDS             9500..11218
                     /locus_tag="KR314_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0326646.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLNIGSYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMKSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDNIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11249..13249
                     /locus_tag="KR314_000237"
     CDS             11249..13249
                     /locus_tag="KR314_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0326647.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKEKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNVRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTKKEFEEILRILYERRESSSGVGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13597..13842
                     /locus_tag="KR314_000238"
     CDS             13597..13842
                     /locus_tag="KR314_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBH0326648.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13877..14602
                     /locus_tag="KR314_000239"
     CDS             13877..14602
                     /locus_tag="KR314_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBH0326649.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEEENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14624..15937
                     /locus_tag="KR314_000240"
     CDS             14624..15937
                     /locus_tag="KR314_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0326650.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVCGENLKKIV"
     gene            16236..16682
                     /locus_tag="KR314_000241"
     CDS             16236..16682
                     /locus_tag="KR314_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0326651.1"
                     /translation="MGGETHFLSFGKDEYKISLEEQENAIESAKASDNSVKLIAFIDD
                     EIVGIATIDSNQKAKGKHVGVLGIVVKEKYWGIGLGKRLMLDLIEWCKSNGITKKITL
                     VTNEENYNAIGLYKKVGFEVESILKKECYYNGVYTDLIGMSLLLGI"
     gene            16771..18756
                     /locus_tag="KR314_000242"
     CDS             16771..18756
                     /locus_tag="KR314_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBH0326652.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTITLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIEEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18980..19948
                     /gene="spoIIIAA"
                     /locus_tag="KR314_000243"
     CDS             18980..19948
                     /gene="spoIIIAA"
                     /locus_tag="KR314_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBH0326653.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIDD
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19938..20459
                     /locus_tag="KR314_000244"
     CDS             19938..20459
                     /locus_tag="KR314_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBH0326654.1"
                     /translation="MQIKIIIIALLVGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20476..20673
                     /gene="spoIIIAC"
                     /locus_tag="KR314_000245"
     CDS             20476..20673
                     /gene="spoIIIAC"
                     /locus_tag="KR314_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBH0326655.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20689..21075
                     /gene="spoIIIAD"
                     /locus_tag="KR314_000246"
     CDS             20689..21075
                     /gene="spoIIIAD"
                     /locus_tag="KR314_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBH0326656.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21160..22365
                     /gene="spoIIIAE"
                     /locus_tag="KR314_000247"
     CDS             21160..22365
                     /gene="spoIIIAE"
                     /locus_tag="KR314_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBH0326657.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22425..23042
                     /locus_tag="KR314_000248"
     CDS             22425..23042
                     /locus_tag="KR314_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBH0326658.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            23054..23608
                     /locus_tag="KR314_000249"
     CDS             23054..23608
                     /locus_tag="KR314_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBH0326659.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKKQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23635..24324
                     /locus_tag="KR314_000250"
     CDS             23635..24324
                     /locus_tag="KR314_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBH0326660.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSDSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24434..24796
                     /locus_tag="KR314_000251"
     CDS             24434..24796
                     /locus_tag="KR314_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBH0326661.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24881..25387
                     /gene="nusB"
                     /locus_tag="KR314_000252"
     CDS             24881..25387
                     /gene="nusB"
                     /locus_tag="KR314_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBH0326662.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25371..26441
                     /locus_tag="KR314_000253"
     CDS             25371..26441
                     /locus_tag="KR314_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBH0326663.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26438..27643
                     /locus_tag="KR314_000254"
     CDS             26438..27643
                     /locus_tag="KR314_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBH0326664.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVMVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27645..27860
                     /gene="xseB"
                     /locus_tag="KR314_000255"
     CDS             27645..27860
                     /gene="xseB"
                     /locus_tag="KR314_000255"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBH0326665.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27863..28750
                     /locus_tag="KR314_000256"
     CDS             27863..28750
                     /locus_tag="KR314_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBH0326666.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28780..29217
                     /locus_tag="KR314_000257"
     CDS             28780..29217
                     /locus_tag="KR314_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBH0326667.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29251..31116
                     /locus_tag="KR314_000258"
     CDS             29251..31116
                     /locus_tag="KR314_000258"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBH0326668.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANNNED
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31249..32061
                     /locus_tag="KR314_000259"
     CDS             31249..32061
                     /locus_tag="KR314_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBH0326669.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEYTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            32081..33760
                     /gene="recN"
                     /locus_tag="KR314_000260"
     CDS             32081..33760
                     /gene="recN"
                     /locus_tag="KR314_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBH0326670.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLKSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGSFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33975..34841
                     /locus_tag="KR314_000261"
     CDS             33975..34841
                     /locus_tag="KR314_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326671.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECTIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGDISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAQLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34932..35423)
                     /locus_tag="KR314_000262"
     CDS             complement(34932..35423)
                     /locus_tag="KR314_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0326672.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIVEAKKIGYTHMRLDTLPSMKSAQGLYEKFGFYDIELYTYNPIEGARYME
                     LKL"
     gene            complement(35484..35924)
                     /locus_tag="KR314_000263"
     CDS             complement(35484..35924)
                     /locus_tag="KR314_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0326673.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGCGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFKFNGELDYGGEKVMVLKSN"
     gene            36085..37143
                     /locus_tag="KR314_000264"
     CDS             36085..37143
                     /locus_tag="KR314_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBH0326674.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVGYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37285..38109
                     /gene="spo0A"
                     /locus_tag="KR314_000265"
     CDS             37285..38109
                     /gene="spo0A"
                     /locus_tag="KR314_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBH0326675.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38522..39643
                     /locus_tag="KR314_000266"
     CDS             38522..39643
                     /locus_tag="KR314_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326676.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39661..40536
                     /locus_tag="KR314_000267"
     CDS             39661..40536
                     /locus_tag="KR314_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBH0326677.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIVLKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNSLGNTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40560..41252
                     /locus_tag="KR314_000268"
     CDS             40560..41252
                     /locus_tag="KR314_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0326678.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41260..42084
                     /locus_tag="KR314_000269"
     CDS             41260..42084
                     /locus_tag="KR314_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBH0326679.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            42089..43156
                     /locus_tag="KR314_000270"
     CDS             42089..43156
                     /locus_tag="KR314_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBH0326680.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGKIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43158..43694
                     /locus_tag="KR314_000271"
     CDS             43158..43694
                     /locus_tag="KR314_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH0326681.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43762..44367
                     /locus_tag="KR314_000272"
     CDS             43762..44367
                     /locus_tag="KR314_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBH0326682.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTVSIGIIKFLAK"
     gene            44379..45293
                     /locus_tag="KR314_000273"
     CDS             44379..45293
                     /locus_tag="KR314_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0326683.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45335..46507
                     /locus_tag="KR314_000274"
     CDS             45335..46507
                     /locus_tag="KR314_000274"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBH0326684.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46554..47366
                     /locus_tag="KR314_000275"
     CDS             46554..47366
                     /locus_tag="KR314_000275"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBH0326685.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47570..48895
                     /locus_tag="KR314_000276"
     CDS             47570..48895
                     /locus_tag="KR314_000276"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBH0326686.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEVDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSIKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            49108..50187
                     /gene="mltG"
                     /locus_tag="KR314_000277"
     CDS             49108..50187
                     /gene="mltG"
                     /locus_tag="KR314_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBH0326687.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            50359..51033
                     /locus_tag="KR314_000278"
     CDS             50359..51033
                     /locus_tag="KR314_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBH0326688.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFASIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            51020..52267
                     /locus_tag="KR314_000279"
     CDS             51020..52267
                     /locus_tag="KR314_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBH0326689.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRAPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            52339..54003
                     /locus_tag="KR314_000280"
     CDS             52339..54003
                     /locus_tag="KR314_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBH0326690.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(54100..54384)
                     /locus_tag="KR314_000281"
     CDS             complement(54100..54384)
                     /locus_tag="KR314_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0326691.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(54407..55924)
                     /locus_tag="KR314_000282"
     CDS             complement(54407..55924)
                     /locus_tag="KR314_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0326692.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKACDLDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAIYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            complement(56049..56870)
                     /locus_tag="KR314_000283"
     CDS             complement(56049..56870)
                     /locus_tag="KR314_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH0326693.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETK
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKSLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            57061..58488
                     /locus_tag="KR314_000284"
     CDS             57061..58488
                     /locus_tag="KR314_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HBH0326694.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSINKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKLVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTIAKNNAIVLVGNDSNKSV
                     LKSTIKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKIYN"
BASE COUNT        23522 a         6098 c        10734 g        18375 t
ORIGIN      
        1 taaaaggtga tatattattt atgaaaatcg tttaatgtat acaataatat atctataaca
       61 aaataataaa agatagagtt ttatttagac atttattaca aatcaaaatt tattacatat
      121 tatttataaa aaaatctaag gaggaaataa taatgaaaat attagtatta aactgtggga
      181 gttcttcatt aaaatatcaa ttaatagata tgaataatga agaagtttta tgtataggat
      241 tggttgaaag aataggaata gaaggttcta tattaaaaca tgaaaaagca ggtagagatg
      301 ataaatacgt tgttgaacaa ccaatgaagg accacaaaga tgcaatagca ttagttttag
      361 aagctgttgc tcatccagaa tttggtgcag ttaaagaaat gaaagaaata gatgcagtag
      421 gacatagagt tgtacatgct ggagaaaaat ttgcaacttc agtagtaata actccagaag
      481 tagaagaagc tttaaaagaa tgtatagatt tagctccact tcataatcca gcaaacataa
      541 tgggaataga tgcttgtaaa gctatacttc cagatgtacc aatggtaggt gtatttgata
      601 ctgctttcca tcaaacaatg cctaaatctt catacttata tggtttacct catgagctat
      661 atactaaata tggggtaaga agatatggat tccacggaac ttctcataat tatgtatctc
      721 agagagcagc agagatatta ggaaaagata taaaagattt aaaaatagta acttgtcact
      781 taggaaatgg tgcttctata gctgctgttg atggtggaaa atgtgtagat acatctatgg
      841 gattcactcc attagaagga ttaataatgg gaactagatg tggagatata gacccagcta
      901 tattaccatt cttaatgaga aaagaaggtt tagatgctga tggattggat aaattaatga
      961 ataaagaatc tggagtatat ggaatgactg gaatttctag tgacttcaga gatatagaag
     1021 atgcagctaa aaatggagac gaaagagctc aggctacatt agaagcgtat gttaaaaaag
     1081 tacaaaaata tataggcgct tatgctgctg aaatgaatgg attggatgtt gtagtattta
     1141 ctgcaggcgt tggtgaaaat ggaaaggcta taagagcaga tattgcttct aacatggagt
     1201 ttttaggaat gaaattagat aaagaagcta atgatgttag aggaaaagaa acagtaatat
     1261 ctactgctga ttctaaagta aaaatgcttt taataccaac taatgaagaa ttaatgatag
     1321 ctagagatac attaagattg gtaaaataat taaattaaac ctatgcctat caagtaaaaa
     1381 tacttgacaa acaaatgcta gatttgtata ataaatttgg tgatatgttg aggtgaactt
     1441 aatgaaagtt agcatagaaa aaataaatag aaaagaaact gacaaaatag acttgaattt
     1501 ttgtgaaaaa attgatacta ttagttattg tgatgagatt tacaaattag tatcacctgt
     1561 aaatttaaaa gggaaagtgt caaagactaa taaaggtttg tatctagata tagatgtcaa
     1621 ttttacaatt gttgacaatt gttcaagatg tcttaaagaa gtagaaatac cactagagta
     1681 ttctatacaa ggttttttag taaaagaaga agattatgat gaagatgagt ttgaggaatt
     1741 tgatcctttt atattcgatg gtgaagaaat cgaccttatt gatataatag aacagacatt
     1801 agattttaat gtacctcata aagttctttg tagtgaaaac tgtaaaggtc tttgtcaagt
     1861 atgcggggca aacttaaatg aagaagagtg ttcttgcagt gaaattacaa acgatgagga
     1921 atacatagat cctcgttttg ctaaattaaa agatttattt aattaaattt tgactaagga
     1981 ggtggcgtaa atggcagtac caaagcgtaa aacgtctaaa tcaaacacaa aaatgagaag
     2041 agcagctaac tcaaagatgg aagcaacagg atttgtaagt tgcccacaat gtcatgagcc
     2101 aaaattacca catagagtgt gtccagactg tggatattat aaaggtaaag aagttgtgtc
     2161 taagtaatag cttctaaaaa gcatgtagag tatactctac atgttttttt tgtacatata
     2221 aaaaaataag atgatttact agagaaaata agtggatttt atgcatatta aaaaatagaa
     2281 aaataataat ataagtattg aaaatttttg tgtgttacta tatactacta ttagtagctg
     2341 gtactaatta cagctataaa atttggaggg aacatgaaaa agaaaagtaa agcccaaaga
     2401 caaaaagagc ttatagatat gctaaagaca gatccttttt atactgatga agaattatca
     2461 agtctgtttg atgtaagtat acagaccata agattagata gaatgagtct aaacatacca
     2521 gagctaagag aaagagtcaa atcaatagca gaaactcaga gttcaaaagt aaagacttta
     2581 ggagtaaagg aaattacagg agaaatcatt gatttatctg tagggcgatt aggtatatct
     2641 atgttagaag ttacacagga catgatttac tctaagacta atactttaaa agatacgtat
     2701 attttttctc tagcagattc attggcaatg gctataatag atgcacctaa ggttattatg
     2761 agagttgcaa atgtaaaaag ttttaagttg atagaacaac aagatagact tatagcaaaa
     2821 gcagaagtgt atagaaatat agataaaaag cattatgtaa aagttgttat caacaataaa
     2881 gcccaagagc aaatatttag aggaaagttt atctttgaag aattagatta ggagggaaat
     2941 tatgaaaata gtaattgatg gaatgggtgg agataatgca cctaaatcta atgtagaagg
     3001 cgcagtaaat gctataaaag aatatcaggt tgatttaata atcacaggag ataaagattt
     3061 acttgaaaag gaattttcaa attatgaatt tgatagaaat aaattagaga tagttcatac
     3121 tactgagatt atagaaaacg aagataaacc agtaaaggct ataagaagta aaaaagattc
     3181 atctatggta gtagcattaa atcttgtaaa agaaggaaag gcagatgcta taatatcagc
     3241 tggaaataca ggagccttac ttgctggtgg attatttgtt gttggtagaa taaaaggaat
     3301 cgatagacct tgcttatgtt cagcaatacc aaatgtaaag cgaggaatga cacttatagc
     3361 agactgtggc gcaaatgcag attgtaaacc taagaatcta gttgaatttg cagctatgag
     3421 taatatttat tcaagaaaag ttttaggtct tgaaaatcct aaggtagcat tagcaaatgt
     3481 tggactagaa gaaggcaaag gcaatgattt agtcaaaagg tcatatgaag aaattaaaaa
     3541 attagattta aattttatag ggaatgtaga agctagagaa gttataaatg catacacaga
     3601 tataatcata tgtgatggat ttacaggtaa tatattactt aaatctgctg aaggtgtagc
     3661 actttcagtt atgagtctta taaaggaaac ttttatggcc agtacaaaaa gtaaaatagg
     3721 agcattactt ataaaggatg atttaagaaa attaaaaagt tttatagatt attctgagta
     3781 tgggggagct ccacttttag gtctaaatgg aggagttata aaagctcatg gtagttctga
     3841 tgctaaagct ataaaaaatg ctataaatca aggaataaaa ttttcaaagg gaaaagtcgt
     3901 tgaagatatt aaccaattta taagcaaata caatgaagaa aataaaaata acgaagatga
     3961 ataaactcgt tttggaggtt taatatataa tgaatacaaa ggctggaata ttaggagtag
     4021 gaagttatct acctgagcaa tcatatgata attttcactt cgaaaaaata atggatacat
     4081 cagatgagtg gataagtact agaactggta ttaaggaaag aagatttgcc aaagagtctg
     4141 aagcaacttc tgacttagca tctaaagctg ccttaaaagc tatagaatgt gcaaagttaa
     4201 acgtagagga tatagagctg attatattag ctacaattac tcctgatatg tctttgcctt
     4261 caactgcatg tatagtacaa gatgcaatag gagcagtgaa tgctactgca tttgatatat
     4321 cagcagcttg ttctggattt gtatatggag taactattgc aaagcaattt gtggaaacag
     4381 gttgctataa aaatgtgctt gtaataggag cagaaacttg ttctaagttt ttaaattatg
     4441 atgatagaac aacagcagta ttgtttggag atggagctgg agcagctgtt attggtcctg
     4501 taaatgaggg tggaatactg tctacacata tgggctctga tggtaaagga aaagattgtt
     4561 taaaagtacc tgctggaggt tcaagactta aggcaagtaa agagactgta gaagctaatc
     4621 tacatacaat agaaatggca ggtagtgatg tgtttaaatt tgcagttaga aagatggcag
     4681 aaacttcttt aagagctctt gaaaaagcaa acctaaatac aactgatata gactacttag
     4741 tgccacatca agctaatata agaatcatac aagcttcttc taagagatta gagcttgata
     4801 tgaaaaaagt ttatgtaaat atagacaaat atggaaatat gtctgctgca tctataccag
     4861 tagcattaga tgaagcttat agagaaggta aaattaaaaa gggtgataat gtagttttag
     4921 ttggctttgg tggaggtcta acttgggggg cttcagttgt caagtggact ttatagtata
     4981 aaatatctaa aatgatttaa ttaacaaaat taaacttata cacaaaataa agttgattaa
     5041 taataaaaag tttactttta tgatttcaag tgaaggagaa ttgtatgaat aaaatttgca
     5101 aaatattaaa tataaaatac ccagttatcc aagggggaat ggcatgggta gctactgcat
     5161 cattagcaag tgctgtatct aatgcaggag gacttggcat aatagcagca ggaaacgcac
     5221 caaaagaagc tataaagaaa gaaattgttg agtgtaaaaa attaacagat aaaccttttg
     5281 gagtaaatgt aatgcttatg tcgccatttg ttgatgatat aattgatttg attatagaag
     5341 aaaaagttca agttattact actggtgctg gaaatcctgc aaaatatatg gatagattaa
     5401 aggaagctgg aacaaaggtt attcctgtag tacctacaat agctttggca caaagaatgg
     5461 aaaagttagg agctacagca gtaatagcag aaggtactga aggtggagga catataggag
     5521 aacttactac tatggtctta gttccacaag ttgctgatgc tgtaaacata cctgtaatag
     5581 ctgctggagg aattgtagat ggtagaggaa ttgcagcatc atttgcatta ggtgccagtg
     5641 cagttcaagt aggaactaga tttatttgca gtgaagagtg ttctgtgcat tcaaactata
     5701 aaaacttagt actaaaagca aaagatagag atgcaattgt aacaggaaga agtactggtc
     5761 atccagtaag aacattaaaa aataaactat caaaagaatt tttaaagatg gaacaaaatg
     5821 gagctactcc tgaagaattg gataaaaaag gtacaggagc tttaagattt gcaacagtag
     5881 atggagacat agaaaaaggt tcatttatgg caggtcaaag tgctgctatg gtaaaagaaa
     5941 taacaccttg taaggaaatt atagaggcta tggtaaatca agcaagagag attatgccag
     6001 caatagaact gtaaatcagg aggagcaaga tgggaaaagt tgcactagta ttccctggtc
     6061 aaggagctca atatgtaggt atggccaagg atttatatga aaataatgct gttgctaaaa
     6121 gtgtaataga tgaggctagt gatgccttaa atatggattt aaaaaacctt atgtttaatg
     6181 gaaatgaaga agaactttct aaaactgaaa acactcaacc tgctatggtt acacatagtg
     6241 tagcagtatt aaaagcggta caagctcaaa tagatttaaa atataatgca tgcttaggat
     6301 tatccttagg agagtatagt gcgttggttg cagcagatgc aattgacttt aaagatgctg
     6361 tatgtttagt taaaaaaaga ggtaagttta tgcaggaaac agttccagca ggtgttggag
     6421 ctatggcagc aattttaggt ttagatagaa atgtacttga aaatgtggtt aaagatatac
     6481 aaggaggtat tgtagaagta gcaaattaca attctcctgg acaaatagtt atatctggtg
     6541 aaaatgaaaa gatagaagaa gcaatggtaa aatgcaaaga agcaggagca aaaagggcag
     6601 ttaaattaaa tgtaagtgga ccttttcact catcaatgtt aaaagaagct ggtgtaaaat
     6661 tggccagtga attagaaaaa gttaacataa ctaaacctaa agttgatgtg gtggcaaatg
     6721 taaatgcaga ttactataaa aacgaaggta aagacctgct tataaaacaa gtaagttcat
     6781 ctgttctttg ggaagattct atagaaatac ttttaaatga tggatatgat acttttatag
     6841 aaatgggacc tggaaaaact ttaaaagcat ttattaagaa gatagcttcc aataaaaaag
     6901 caaatgtcaa tatttataat atagatggta tagattctct taatgaattt gtacaaaatt
     6961 atagaaatgg agagatataa tgataaatct tacaggacaa gtagcagttg ttactggtgg
     7021 ctctaggggt ataggaaaag aaatagcaaa aaaactagca tcttttggag ctgatgtagt
     7081 aatcaattat acttctaaag aagatgaagc actaaaaact aaaaatgaaa tagaaagtat
     7141 gggggtaaag tgtacctcta taaaatgtga tgtgtctaaa tttgatgaag tgaatcaaat
     7201 gatagattct gttgtaagcg aatttggaaa aattgatata ttggttaata atgcaggcat
     7261 aactaaagat ggtctgctta tgagaatgaa agaagaagat tttgatagag ttatagatat
     7321 aaacttaaaa ggtgtcttta attgtacaaa agcagttact aaacctatga tgaaaaagaa
     7381 gtatggaaga ataataaata tgacttcagt agttggaatt atgggtaatg cagggcaaac
     7441 taattattgt gcatcaaaag caggtgtaat tggatttaca aaagcttctg caagagagtt
     7501 agcatcaaga aacataaata taaatgcagt agcacctgga tttatagaaa cagatatgac
     7561 tagagtacta agtgatgatg taaaagaatc aacactagca aacataccaa agaaatctta
     7621 tggtaaacca gaagatgtag ccaatgccgt agcattttta gttagtgaca tgtcaagtta
     7681 tataacagga caagtaataa atgtagatgg tggaatggta atgcaataat aatttaaaaa
     7741 gtagagaaat ctattaaaat aaaaaatttt aggaggtaca caatgtttaa taaagttgta
     7801 gaaataataa tagagcaatt aggagtagaa gataaagaaa taactatgga gacatcatta
     7861 atgaaggatt tagaagcaga ttcattagat gcagtagaaa taataatggc acttgaagat
     7921 gaatttggta tagaaatacc agatacagaa gcagaaaact tcaaatctat aggagatata
     7981 gttaagtata tagaagctaa taaatagtcg agctttaaaa aagtggaggt atccgatgaa
     8041 taaaagagta gtaataacag gacttggatg tgttacgccg ttgggaacgg gaaaagaaga
     8101 gttctggagt aatataaaat ctggagtatc tggtatagat aaaataacta attttgatgc
     8161 tagcacatat caaactcaga tagcaggaga agttaagaat tttcatccag aagaatatat
     8221 aagtaaaaaa gaattgaaga gattagataa atttgctcaa tttgcaatag tatctgctaa
     8281 attagcagtt gaagatgcaa atcttgattt agataaagtt gatagagaac gttttggtgt
     8341 tataataggt tctggaatag gtggagttga agctatagaa actcaacata aaattctttt
     8401 ggaaaaagga aataaaagag taagttcact ttttgtacct atgatgatag gaaatatggc
     8461 agctggtcaa gtttcaatat ttttaggagc aaaagggcct aacactaatg tatgtacagc
     8521 ttgtgcatca ggaactcatt caataggtga tgcttttaag gtaattcaaa gaggtgatgc
     8581 agacatcatg gtagcagggg gctctgaagc tgctgtaaca ggtcttgctt ttgctggatt
     8641 ctgtaacatg aaggctatgt caactagaaa tgatgaccct aaaactgcat caagaccgtt
     8701 tgataaagac agagatggtt ttgtaatggg agaaggagca ggtattgtta tcttagaaga
     8761 cttagaacat gctttagcaa gaggggctaa aatatatgca gaagtagttg gatatggttt
     8821 aactgctgat gcataccata tgacaactcc agctgaaaat ggagagggag cagctaggtc
     8881 tatgaatatg gctttgaaag atggaaatgt tccattagaa gaagtagatt atataaatgc
     8941 tcatggaact tcaacttatt acaatgattt atatgaaact atggctataa aaactgtatt
     9001 tggagaaaag gcatatgact tatgtgtgtc atcaacaaaa tctatgactg gtcacttatt
     9061 aggagcatca ggtgctatag aggctgttgt ttgtgctatg agtatagaag atagttttgt
     9121 gcctccaact ataaacatac aagaagttgg tgaagatttg gatttagatt atgttccaaa
     9181 tcaaggtaaa gaaaaaaata ttagatatgc actatcaaat tctttaggat ttggtgggca
     9241 taatgctaca atagtactaa aaaaatatgt ataaataaat attaaatata aaactaaaaa
     9301 acaagcactt gttttaactg tataaattta tattagctta aatttaaagt ctcctaaatt
     9361 attttaggag acttttttat taagatttaa gtctagcata tgttgtaaat cattttgata
     9421 tattgtatta ttaattatta agaggttatt tatatatgca agttttcatt ctacaatata
     9481 caaaaggagg actaaaattt tgaattttag actaccaaag aaaaggttac ccaaaaagat
     9541 aatttttata tgtataatac ttattagtat tattttgttt ataaagtatg accaaaatgg
     9601 attgaaagaa agagaaatta taaataaggg aaaatatatg tctgaaagcc ttgtggcaga
     9661 taaggagtat gatgaagcaa aaaaagtggt taagacttct ttcgaatcta ttccagaaaa
     9721 aaaatacaat aaagtatata atgaagtttg ggatatgtta aagactattt catatgttcc
     9781 agatggaacg aagatagtca ttgattcttt agaaaagatg agagaatcag atgtgaaatt
     9841 atctgatgaa tgtagattta atatagaaaa aagattagca tcagtgtata taatggataa
     9901 taattattct aaggcagtag atttaactgt aaagtctata aaattggctg agaaattggg
     9961 gaataattat gaaaaagcaa gactagatgt tgatttggcg agcatatttt taaatatagg
    10021 tagctatgaa acaggagaga aattaataaa ggattctttt aaaatagata ttgatgaagg
    10081 tgaaaaaaat ggaatggtta gattatatgc tattataaat ctagcagaaa tatatgtaga
    10141 agagggagaa tatgataaag ctattgaaat aagtagcagg ataaaagatt ataaaaagtt
    10201 tgtaaatacg gataattaca atgattttga tataatggcc tctataatga agtcaaatgc
    10261 gtatgtgggc aaaaataata tacaaaaggc aaagactttt ttagaaaaag ctgattatct
    10321 tataaaatca gatagaacag tttatatatt agataaagat atgtactatt atatggcaat
    10381 gggaaactta gaatataaaa gcgaaaatta taattctgca atagataatt ataaaaaaag
    10441 tttagagata tctaccaaaa gaaagttaac tcaggagaaa ataaaaaatt taaataagat
    10501 attaaatata tatgaaaaac aaggaaatat agaatcttta aatgagtatc aaaagatgtt
    10561 aataaatgaa tataaagaga acaaaagtat aagagattca gagactagtt tctatataat
    10621 tgataaagta tcaaacgaaa gtaagctatt tgaaaaaact aaaaaagaaa taaaatatta
    10681 taaaatatta tttacagtaa ttttaattgc tatattaggg tctacttttt tatacaaccg
    10741 attgaagtat tttaaagcac aaaatttaca tgatggtctt acaaatgttt ataatagaaa
    10801 aagttttgat attatgtatg aaaagtacag agagaaagat gataattttg cacttgtgat
    10861 gatagatatt gataatttca agctcataaa tgataattat ggacataaat ttggtgacac
    10921 tgttataaag ggaataacaa ataccatttg tgttatgtta gaacaagatg ataaagtctt
    10981 taggtacgga ggagaagaat tttctgtatt aataagaaat aaatctggtg aggaagtagc
    11041 agatataatt gacaatataa gggttgcaat tgcaaataaa aagtggaatg atgatgtgac
    11101 tgtgactata agtgcaggtg tagctcatat ttcagatagc aaagatgtac tagaagaagc
    11161 tgataaaaat ttatataaag caaaacaact tggtagaaat aaagtagtat ataagtaatt
    11221 atatactata taaataagag gtataaatat gaaacaatca atagctatag taactgataa
    11281 aataactaaa ctatcatctt tcttagaaga aaatatacgt ttggttttag gtaattatat
    11341 tgatataaac cactattatc tggaagtttt aaaagagaat gataaaatac aaggtgattt
    11401 tgtactagta atgaatgatg atagattaaa cagtatgaga aagcatttag tacaagatag
    11461 taagattata gtgataagga gaacacttaa agaaaatgaa atatatggat tattttcaat
    11521 accaaatgga actgaagtat tggttgtaaa tgatacaaaa gaaactacct tagaaacgat
    11581 aagcttattt tacaaaatag gagtaaaaaa tttaaagttt actccatata ttgaaggaaa
    11641 aatttataaa aatatagaca tagctataac gcctggagta aaggaaaaag taccaagtta
    11701 tataaataaa gtaatcgacc ttggtaatag atatattgat atatctactt ttatagaaat
    11761 aataaatggt cttagaattg atgttaaaga aatacgtaaa aatttaatgg catattctga
    11821 gagtttaata agtttagata atggagttaa agataattat aggcaactgt ttttaaaaat
    11881 agaagaacaa gatgttatat tgaatctatc aaaagatgga ataatattta cttcagcaga
    11941 tggtataata aacacattta ataaagaagc tttaaaaatt ttaggtataa atgaaaatcc
    12001 acaaggcaaa aacatagaag atatcatttc tgaagattta aatattttac ttgaagacaa
    12061 agaaataatt gatgaagtgg tatttacaaa taaaaaatat ataaatgtaa ataaaaaaag
    12121 tattttttct atggggaata aagctgggat atattataac ttacaagaaa taacgtatat
    12181 aaaaaaatta gaacaaaatc ttactaataa attaagagaa caaggtcaga tagcaagata
    12241 tacatttaaa gatataaaaa ctaaaaataa aaatatggta aagtgtgtag aattggctaa
    12301 aaaaatatct aaatcagacc tttctgtatt aataatagga gaaagtggta ctggtaaaga
    12361 attattatca caatcaattc ataatgcttc caatagaagt aatcaaccat ttatagcggt
    12421 taattgtgca gcagttccag atagtctatt agaaagtcaa ctgtttggat atgaaaaagg
    12481 ctcttttaca ggagctttaa gagaaggaaa aaagggtcta tttgaacttg ctaataatgg
    12541 aactatattt ctggatgaaa ttggagatat gcctccattg ctacagacaa agttactaag
    12601 ggtattacaa gaaaagcagg ttatgcctat aggttcccat aatgttataa acatagatgt
    12661 tagggtcata gcagcaacaa acaaaaactt actaaagatg attagagaag gaaaatttag
    12721 agaggacctg tattatagac taaatgtatt gccaatagat gtaccatcat tgaggaatag
    12781 aaaagaagat ataataacac ttatgaattt ctttttgaaa aacaatgtaa gactttcgcc
    12841 agaagttata actattttag aaaaatataa ttggccagga aatataagag agttacaaaa
    12901 tgttgcttct tatgtaagtc taatgtgtga tagtatggtt tttagagatg acttaccacc
    12961 atatataagg gtagaatgtg ctaacaatta tgaagatgga tttggttata caaaaaaaga
    13021 atttgaggaa atactgagga ttctatatga aagaagagag tcatcctctg gagttggaag
    13081 agggtttata atcgaaaagc ttatacagga agggattgag atttctgaaa gcaaagttag
    13141 aaaggtgcta tcttatttaa gtgaatgtgg atatatatca tctaaatctg gtagaggagg
    13201 aagtcaaata acctcagagg ggataaaact ttataataat ataaattaag ctaagtattt
    13261 gatattgaaa tcacatattt tgaatttaga tatttaaata ataaactttt atatttatag
    13321 agattaattt atactatgtc agaaagaata ttgtaattta taattaaata ttaataggtt
    13381 tttaaaatag gattagatag gtaataggtt aagttagaat aacccattac ctatttttat
    13441 ttgtaagaaa agtgtacata gagttaaaag taaaaaatac aactaataaa actattgaaa
    13501 acactagata aataataaat aaaaataatt ataaattttg gcatatatat tgctaatcat
    13561 agagataaga cgacaaaata attgggaggt ttaatgatga aatacaaaac aacagggact
    13621 tgtgctacag aaattgaatt tgaagtaaaa gaaaataaag ttacaaatgt aaactttata
    13681 ggtggatgtg atggaaattt aaaagggcta aaagtattag ttgaaggcat gaatatagaa
    13741 gatgtaatac aaaagttaaa aggaatagag tgtaaaacaa agccaacatc ttgcccagac
    13801 caactgtctc tagcattgga aaattatatg aatataaagt aatacataaa acaatcgatt
    13861 agttaggagt ttaattatga aaccaataat agggatttta ggaaatttaa taataatgga
    13921 aaatggtatg tttccaggtc ttgaaaggtc atatgtaaat aatgattata taaatgcagt
    13981 gctaaaaggt ggcggtagtc cagtaataat tcctgtaaat acagataaag aagttataaa
    14041 aaaacagatt gaaatggttg atggagtctt aatatctggg ggatgggata tcaatcctca
    14101 actgtatgga gaagaaacaa gagaagaaac gacatttata tatccagaag tggatgaatt
    14161 tgatttaatt gcaataagta tagcattaga acttaaaaag cctatattag gtgtatgtag
    14221 gggactacaa attttaaatg taagtttagg aggaacttta tatcaagata ataatcttat
    14281 agaaggaagt tatataaaac atacacaatc ttctaaaaga catgtagcta ctcataaagt
    14341 cgatgttaaa gaaggaagta ttttagaggg aattttagga aaacaattac ttacaaatag
    14401 ttatcatcat caaagtgtca atcagcttgg aaaaggatta aaagccatag catactcaaa
    14461 agatggtata atagaagcta ttgagaaaga ggaggaaaat tttgtagtag gagtacagtg
    14521 gcatccagaa atgatggtag attactgtga taaaatggaa aaactattta aatattttat
    14581 aaatatttgt tcaaatttat aatatacctt ggggggattg aacatgaacg aaaagaacaa
    14641 aatgggatta ataagtatta tacttttagg tattaatgca gtagtaggag ctggagtttt
    14701 tctattacca ggagatgcaa tgaaatcatt tggtgtagca agtatatttg tatatatatt
    14761 tgatatgttg ttagttttat ctatggcatt ctgttttgct gaggttgcag gtaaattcaa
    14821 taaaaatggc gctgcctatg tatataccaa agaagctttt ggagattttt gtggttttga
    14881 agtaggtctt atgaaatggg taataggatg tatttcttgg ggagctttaa tagtaggttt
    14941 tcctacatca ttatcagcag tatgggctcc agcaggtgaa ccacatatac aaaaaattat
    15001 aattgtagcc atgatagttg ggcttacaat tataaactta ttaggtgtat ctctatcaaa
    15061 aattgtacaa aatgttataa ctgtaggtaa actaattcca ttaatactat ttataggaat
    15121 aggtatattt tttataaaag gagttaattt tacttcttca acaatggtac ctccaggagc
    15181 tggagcaaca gagtttggag cagcagcact tttaatgttt tattcattta caggatttga
    15241 atctatagca gttgcagcag aagatatgga aaatccacaa aaaaatatac caatagcaat
    15301 tataagtgta atagtaattg catcaataat atatatatta aatcaagttg tatgtgttgg
    15361 tatactagga gattcgttat ctagtacatc tacaccagtg gcagatgctg caagaatatg
    15421 ctttggaaat atgggagctg gattggttac ctttggaaca ttggtttctg ttggtggaat
    15481 ttgtatgtgt ggtgcttttg taaatccaag aagttgtgta gctttagctg atgataaaat
    15541 gttaccaaga atatttgcaa gaaaagataa aaaaggtaca ccatatgtag cgataatagc
    15601 tactatgtta atcactatac caatagctct atctggaagt tttgctgaat tagcagcaat
    15661 aagtgcagtt gctagattca tacaatacat accaacatct ttatccgtat tagtgtttag
    15721 aaagaaaaga ccagaattag taggtacttt taaaactcct tttggagcag tgattcctct
    15781 aattgctgta tgtgttggaa catggttgtt attccaagca tctatgcatc aattaattat
    15841 gggattagga gctttggcaa ttggagtacc attatacttt ataatgaagt cttataatag
    15901 aaaagtctgt ggtgaaaatt taaagaaaat agtttaatta tgttataaga atttattgag
    15961 ttaaaattag acattggcag aaaattaaat acaaataagt atctataagg ctatattaaa
    16021 atattattaa ttttttattt taatatagcc ttatattgtt atcaataaat gaaaaatatt
    16081 gtaacaacag aagaacaata gtattatgtt tatagataaa aaacttatta ggaggtaaat
    16141 ttatgccaac aataacatta aaaaatggtg tagacgtact tattagagaa ggtgtaagag
    16201 aagatgctta aagcattata gatttttata atgaagtggg tggagaaact cattttctgt
    16261 catttggaaa ggatgaatat aaaatatctt tggaggaaca agaaaatgct atagaatcag
    16321 caaaagcatc tgataattct gttaaactga ttgcatttat agatgatgaa atagttggga
    16381 ttgcaacgat tgattctaat caaaaagcaa aaggaaagca tgttggagta ttaggaattg
    16441 ttgtaaaaga aaaatattgg ggaattggat tgggtaaaag gcttatgtta gaccttatag
    16501 aatggtgtaa atcaaatgga ataaccaaga agataactct tgtcactaat gaagaaaatt
    16561 ataatgctat aggattatac aaaaaagttg gatttgaagt agaaagcata ctaaaaaaag
    16621 aatgttatta taatggtgtt tatactgatt taataggaat gtcactttta ttgggaatat
    16681 aattagtgta cattttatct cgaaaaaagt atattaagta tggtataatt aaaaaagaat
    16741 ataacttaat ataattttgg aggacgaatt atgaaaaatc tgtgtaaaat atcagatgat
    16801 tatttaaaca gcaagcttaa gtatttaaag ttattatcaa aacaatatcc aagtatatca
    16861 aaagcaagta cagaaataat aaatttagag gctatattaa atcttccaaa gggaacagag
    16921 cattttataa cagatgtaca tggtgaatat gaaccatttg tacatgtatt aaaaaatggt
    16981 tctggtgtaa taaagagaaa aatagaagaa ttgttttcaa atacaataag agacagcgaa
    17041 aaaaaaatgt tagcaactct tgtatattat cctgaacaaa agttagacct aataattaag
    17101 caagaggaaa atatagatga tttttataga ataaatattt atagacttat agaactttgt
    17161 aaatatgctt ctagcaaata tactaggtct aaagttagaa aactgttacc agaaaatttt
    17221 aaatacataa tagaagaact tttgcatgag catgttaaaa gtgaacataa agaagagtat
    17281 tataaaagta tagttgaaac aatagttgac ataggaatag ctaaggagtt tataattgct
    17341 atttctacag ttatacaaaa attggttgta gatagacttc atgtaatagg agatatttat
    17401 gatagaggtc caagaccaga tataattgtc gataagctta ttgaacatca ttgtgtggat
    17461 attcaatggg gaaatcatga tatattgtgg atgggtgcag cctcaggtga aaaaacttgt
    17521 atagcaaatg cactgaggat atcagctaga tatgcgaatt tagacattgt agaagacata
    17581 tatggaataa atctgttgcc tttagccact tttgctatag aaatgtataa agatgaccct
    17641 tgtaaagaat ttattccaaa ggttaatgac caaagtgtaa caacaacaga aaagtcttta
    17701 atggcaaaaa tgcacaaagc aataagtata attcaattta agcttgaagg tgaggttata
    17761 agacgaagac ctgaatttga aatggaacat agacttttgc ttaatatgat aaactatgat
    17821 gaaggtacga ttactttaaa aggtaaaaca tataaattaa aagatactta tttacctaca
    17881 atagacaaaa aagacccata taaattaact atggaagaaa gaaatgttat tgataaatta
    17941 gtatcttcat ttagaggtag tgaaaaatta caaaaacatg tttcattttt gttttctaaa
    18001 ggaagtatat atttaaaggc taactcaaat ttactgattc atggatgtgt accattaaat
    18061 gaagatggaa gctttatgtc tatgaatata atgggaaaag agtataaagg taaggctctt
    18121 atggataaga tggagtctct agcaagagaa ggatttttct ttaaagataa ggcagaagaa
    18181 aaactatatg gtatggatat tatgtggtat ttatggactg ggaaatgttc atcattattt
    18241 ggtaaagatg atatgactac atttgaaaga tactttatag aagaaaaaga gactcataaa
    18301 gaaaataaga atccatattt taaacttaga gaaaatgaga tggcttgtaa aagattattt
    18361 gaagaatttg atttagagct tgatgaatcc catataataa atggtcatgt tcctgtggag
    18421 agtaaaaatg gagagagccc aataaaagct aatggtaaaa ttctcgttat agatggtggt
    18481 ttttctaggg cttaccaaaa aactactggt atagctggat acactttgat atacaattct
    18541 agaactttgc aattagtatc gcatgaacca tttaattctg ctgaagaagc cattgcaaat
    18601 gaaagtgata tcttatcgac aactgttgta gtagagcata aggcaaagag aaaaatggtg
    18661 agagatactg atgaaggagt aaaaatacaa gaggaaatag aagatttaaa actactttta
    18721 atggcttata agaaagggct aataaaagaa atgtagatat tatagaggca cttcataatt
    18781 gaagtgtctt cttgttaata cttattaaat atattataaa gattaataat agtagtttat
    18841 attatactta ctaagtgaat ccaagataaa tgggttcact ttttttattt ataaacatta
    18901 ttaaaaaaat atagatgttt tggttatatt ccttgtcctt tataaataga attatataaa
    18961 caaaggagga ggacaagaga tgaataaact ttctgatgaa ataataaact ctctttctac
    19021 aactataaga gaaaaggtag aaaaagtctc aaacaataat ctgaacatag aagaaattag
    19081 gttacgctca caaaaaccat tgatactaaa tgcaaactct aaagattatt tttacaatca
    19141 aaaaactatg acacttgatt taaatcaaca aaactcatat gtagtaacaa gagaagatgt
    19201 agagcaaact tttcaaataa tatgtaaata ttcaatacat tcatttatgg atgacataaa
    19261 aaaaggattc ataactttga gaggaggtca tagagtaggg ttagttggaa aagcaatagt
    19321 agaagatgga caagtcaaaa atataaagca tatatcatct ttaaatataa gggtatcaag
    19381 ggaaattatt ggatgctcgg ataagatttt aagtcatatt ataaagggaa aaaatcagat
    19441 taataatact ttgataatat caccacctca atgtggaaaa acaactttaa taagagatat
    19501 agtaagaaat ttaagtaatg gtaatgaaga ttatggattt aaaggtttaa aagtagcttt
    19561 ggtagatgag cgtaatgaga tagcaggagc ttatctaggt gttccacaaa tggatgtggg
    19621 aataagaact gatataatag aaacttgccc aaaagatttg ggtattacga tgcttttaag
    19681 gtctatgtca ccaaatgtta ttgtgacaga tgaaattggg agtgaaaaag aaataaaggc
    19741 attatacaca gcattaaatg gaggtatagg attaataact actgtgcatg gagattcaat
    19801 agatgatata caaaatagaa aagaattaaa tagacttctg gataaagagt tatttaaaaa
    19861 ggttataatt ctttctgcaa aaagaggagc aggaacgatt gaaaaaattt atgatttaga
    19921 agaaaagaga tggtattttg caaattaaaa taattattat agctctcttg gtaggaagta
    19981 gttatttgat aggtgaacaa atatacaaaa cttatactcg tagacataaa caattaaacg
    20041 atttaataag agttttagaa attttgagga tggatttatc tttcggactt tatacattag
    20101 aagaaatctt taataggata ggtggaaata aagagttttg tttttggaaa tttttctatc
    20161 aaatatcaga aggacttcac aatgaacaaa gtaaaacttt ggaaataata atatcagaaa
    20221 atattgatgt cttaagtaaa gaaacttatc taggcaataa agaaattgag gaactgaaaa
    20281 atctaatact tactttaggg aaaagcgata tagagtcaca acaaagaatg atagatttat
    20341 ctatagaaaa tttgaaaaaa caaacctatg agacaaagga agatataaac aaaaaaggcg
    20401 tattgtataa aaaattagtg acattcatag gtataggtat atgtattata ttgatttaat
    20461 atggggaggt ttattatgga aatatcattg atattaaagg ttgcaggtgt cggtattcta
    20521 atatcagtat tgaatatgat attggaaaaa acagatagaa aagattgggc aggtcttact
    20581 acattagcag gtgtaataat cgtattgggt atggtaataa cagaaataag tgacttgttt
    20641 aatactgtaa gaacaatgtt tcaactttac taaaaaggag ggagtaattt ggaaataatg
    20701 caattaatag gaattgcaat catttcaact acactctgtt tagtaattaa aaaagataga
    20761 ccagagatag caaatttcat agcaataata actggagtaa taatactatt gtcagttatg
    20821 tttaagctaa attttatagt agatagtata caagatttgg ctaacaaggc taacataccg
    20881 actatgtata tatcattaat aataaaatta attgggatag catacttaat ggaatttgcg
    20941 atacaacttt gtaaagattg tggggaagga aacattgctt caaaactaga atttggaggt
    21001 aaaataatag ttatgtcaat gtctttccct atacttttgt ccattgtaga aatggtggtg
    21061 aatataattc catagatgtg tatcttggaa tgaatataag tatattagag aactttcgtt
    21121 gtattgaggt ttatagagtg aagagaggca tggttgctta tgaaaaaaag atttttacca
    21181 atgattatag gttttttatt tactttcttt tttgtaaata tctttgctat tatgatattt
    21241 gctaatgaag taccaagtag tgaagataaa gaatatggtg aaacaaaaaa tagcatagat
    21301 aaatatatag atggtcaatt agacaagcta gatataaatg agattcaaga ctatataaat
    21361 aaagaaatag ttattaatga tgttaattta aaatcatttg taaaagattt aattagtgga
    21421 gaaaaaaata tcttagattt attcaataaa gatggattga agatattgat gtttgatgaa
    21481 tttaaagcaa gtttaaaggt agtagcagta attttggtac tggctctgtt atcatctatt
    21541 ttaaagagtc tggaaaactc tttttcatca ggtgcagtaa gtcaaatagc aacatatata
    21601 atatttatca caatggtatc acttacactt attggtttta aagatgtatt acagatttgt
    21661 tatgatgcaa tagaccatac tgtaggtctt atgcaagtta taatgccaat attaataaca
    21721 tttttacttt taattgggtt cccaataacg tcgactactt tgaatcctat cttcatagga
    21781 ggagtaacat ttataaatgt attttttaaa aattttctat ttgtatctat tactgttgca
    21841 tttggaattt taataataaa caatttatca aaaaatataa ggctaaagag atttttctcc
    21901 tttgtaaaac aaataaatta tgtttctatt ggagctatgt ttactgttta cttaggtctt
    21961 gtatccatac aaggattgta tgtaaccagt tttgataaat tcagtgtaaa gactgctaaa
    22021 tttgctatag gtaattttat tcctgttgta ggtggatttg tatctgactc tgtggatatt
    22081 ttattatctt catctcagct tataaaaaat atatttggag gaatagggct tatcttactt
    22141 gttggaattt gtttacttcc agtaataaaa attttatcag taatagtagt gtacaaatta
    22201 gcagcaataa ttgtagagcc tgttggagag gatggaatat ctaatttttt aaatgaagtg
    22261 gcaaatttaa tgataataat gctagcatca gtaattgcta taacagtaat gttctttgta
    22321 actgtagcta tcttgacctc gataagtgtc gtaagtcaag ggtaatttat atatcagtga
    22381 aaaataatta catttaatct aatgttttta gagaggagga tgctatgtta gagggcataa
    22441 aggcatggat agttagtgtt ttgataggag catttatagt aaatatagta gatatgattt
    22501 taccaagttc taagataaaa ccttatgtca acctagtttt aaactttatg tttgtattta
    22561 ttgtaataac tccagtagta ggattttttt caaaagatat gagtttagaa gatagaatat
    22621 taaaatctat gggaaactac aacaagcagt atgtagatag tacaaatgct ttggcaaaag
    22681 agactggaaa taatagtcta tccaagggat atgaggatgg gttaaaagaa gtacttaaat
    22741 taaaacttga tgaatatggt tatgatttag aagatataga gcttaatggg gcaaatataa
    22801 ataatataaa aataaaagaa aagaataata gtgcaaaaag taataacagt tccaatgaag
    22861 aaaataaaaa taatagtact aaaagagagg aagaaaacat aaactctaat gacaaagaaa
    22921 attctaaaca agtcttcaaa aaaggtacag agtatgggtt gaacctaaat gaagaaaaat
    22981 taaaaaacga cttaataaag gtgctggatg tttctataga agatatacaa attgataaat
    23041 aggagttggg agaatgttga aaaacttaaa tgacaaagat aagagaaagg tatattcact
    23101 aataactata gcaggaatat gtgtggtttc attagtatta ttatcttgtt ttcctagtag
    23161 caaaagtgat aaacaagttg gaaaaacaga gactaataaa aatgctgaaa agcaagtcac
    23221 taaaaaacaa gaaaaagatg atttagagtc aaaattaaca gcaatactat ctaaaattga
    23281 tggtgcagga gacgttgatg taatggttac atttgaatca agtgaagaaa tacaacctgc
    23341 ttttaattca aataatacaa cagaaacaac agaggaaaaa gatgcacaag gtggagaaag
    23401 aacagttacg acttctagtg aaaataaaac aatgataact tcaaattcaa gtgaccctgt
    23461 agttataaaa actacagagc caaagataaa gggagtgata gtggtagcaa gtggagcaag
    23521 tgacccaagt gttaaagaga cactttatag tgcagtacaa acatcattac aagtagctgg
    23581 tcatcaagta gaaatatatt ctaaataaat acatataacc ggggggataa atgaatgaag
    23641 tttaattata agggaagagg atttgtaata ataactttaa ctgcaatgtt agtggtagta
    23701 ggaacagtga attatcaact aagtaagaaa tctttattag aaacatcaaa agagtttaag
    23761 gcatatgaag aagcacagtt gcaaaaaaat actgatgata gtgatagtag taagacagag
    23821 gattctagta atgtagataa acaaggtggt aaggaaagtg ctgatattga tatagtcgat
    23881 agcaaagcta gtaaagtaaa agaaaaggca actgagacaa gtaaggaaat aaaagctcaa
    23941 ttatcatcag agaaaaatat gaaaaaggca tcttatattt tagatatgaa aatgaataga
    24001 gaaaaacaaa gaaatgagtt agttcaagat ttaaatgaaa tgataaataa tccatctaca
    24061 acagaagaat ctagaaaaga agcttctaat atgaaattaa atatagttaa aattcaagaa
    24121 aaagaacttc aaatagaaaa ccttttgagt acaaaaggat atgaagaagc gttagtttat
    24181 ataagcgata acaaagttaa tgtagttgta aatgaagcta aattagaaaa gaaagatgca
    24241 gcaaaaatat ttgatttagt tgcagaacaa gccaatgtga agtatgaaaa tattaaactt
    24301 acaaataata atagtaataa gtaaagtata aacctcagaa ttaactgagg ttattttctt
    24361 gcaaagtaga cttgtatttg gtattattat acttataaaa atagttcctt aaaatcagga
    24421 ggtaagtttt aatatggaag ataataaatt tggacaagta aaaatatcta atgatgtaat
    24481 agctacaata gctggtttag cagcattaga agttgaaggc atagaaacta cagcaacatt
    24541 aacagataag ttgttaaaaa ataatggagt aaaaatacaa atcgaagaag aggatgttaa
    24601 tttagatgta atggttacga taaagtatgg aatgtcaata ccagatacag cttttaaagt
    24661 tcaagaaaat gtaaaaaata ctgttgagac aatgacagga ctgaaggttt ctcaagtaaa
    24721 tatacacatt caaggaatca gctttaagaa ggataaagtt gataaagaag aagcaaaagc
    24781 aactaagaaa aattaatatt agccctctga aaagtcagag ggttttttgc tggtaattga
    24841 ttttatatgg ataattaaac ttaaaggaga attttgattg atgaaaaaag atagggcaca
    24901 aaagagtaca acaagagaat atataatgaa acttatatac caaataaata ttaataaaga
    24961 agattttgaa actttagaag ataaagtaga taatttttta aaagataatt cagaacatat
    25021 tataaataga tataaagaac ttgcacttca atactcaaaa aatacaaatt taaagttaga
    25081 agatactgaa attgaagatg ttatagacaa gaaatatata aatacagtat gcaaagcatt
    25141 aaaagaaaat catgacaaaa tagatgagtt aataaataag catgctaaaa actggacagt
    25201 agatagaatg ccaaaggtag atgtttctat acttagattg tctgtgtgtg aaatacttta
    25261 tttagacact cctaataaag tatcaataaa tgaagcggta gaacttgcaa aaatatattg
    25321 tgatgataag tctcctaaat ttataaatgg tatattggga agtgttgttg atgaaattgg
    25381 gaaataatat aataattgga attgatacta gctgctatac tacttctata gcagctatct
    25441 ctttagataa gaaagttata ttcaatgaaa aaataatgtt agaagtaagg gataattcaa
    25501 aaggtttaag gcaaagtgaa gctgtatttc aacatatcaa taatttaggg attcttagtg
    25561 ataggataaa atcttttaaa gataagttta atgtagaagg agtttgctca tcaaaaaaac
    25621 cgagacctgt tgaaaactca tatatgcctg tattcaatgt aggtcataat tttggaaaat
    25681 tattgtctag tatatatggt tgtaggtttt atgagactac tcatcaagag aatcatattg
    25741 aagcaagttt attaaatagt aaattaaaaa ataataataa atttatttct gttcatatgt
    25801 ctggtggtac tacagagata cttttaacaa gcaaacaaga tagtcatcac aatgtatgtg
    25861 atactaattt aggtaaaatt gccaaaataa gtattaaaaa agatgataaa agtaagttat
    25921 acaataattt tggatataat atagatataa ttggaggaag taaagatatt agctttggtc
    25981 aactcataga tagagtagga attaagcttg gatataaatt tccttcagga aagtatttgg
    26041 atgaaaatgc tttaaattgt aatcttaaaa tagaaagtgg tctgaaaact tctgtaagag
    26101 atgggtatat gaatttatct ggattagaaa atcaggtaaa taagattata aatgataatg
    26161 gagataatac taatcaaaaa gaatacatat ctaaattagt attagattca gtagttagaa
    26221 atatgtttaa atctttagta tatttatgtg aaacttataa tgtaaatgaa gtagtatttg
    26281 ctggtggagt ttctgcaagt aagtatattc ttagagaatt aagcatgaag ttaagaaaaa
    26341 agcatataga agcttatttt acagaaccac aatattcaac tgataatgct gtcgggtgtg
    26401 ctataatagg attgaataac tttttaggag aaagagtatg aaactaagag ctttagatat
    26461 aagtgaagca aactcatata ttaaaagaat tttaataaac gacccaatac tttctaatct
    26521 taaagttaaa ggagaaattt ctaattttaa ggttcatagt agtgggaatg tatatttatc
    26581 acttaaagat gaaacatcaa agttaaactg tgtaattttt aaaagtaatt ttaatcgcaa
    26641 tttaaaatta gataatggtg taaaaattat agctaatgga tatatatcag tctatgaaag
    26701 agatggtgcg tatcaattat acattaatga aattgaaata gagggaatag gaaatttaca
    26761 catagagttt aatagattaa aggaaaaact taataaagaa ggattatttg accctaaata
    26821 caagatacct atacctaaaa tgccaaactc aattggcgtt ataacatctc caacaggtgc
    26881 tgttataagg gatataataa atgtaataaa aagaagatat ccgaaagtta atataaagct
    26941 ttatccagta atggttcaag gagataaatc agctgaagaa atctgtgaag caattagatt
    27001 tttcaatcat atgaagaatg ttgatacact tatagttggt agaggtgggg gttcaataga
    27061 agaactttgg tcttttaatg atgaaatggt agcaagagag gtttttaatt ctcaaattcc
    27121 gataatatca gcagttgggc atgaaacgga ttttactatt tgtgactttg tgtctgatat
    27181 gagagcccca acgccatcag cagcagctga gattgctact ccatcattgg atgatataaa
    27241 ctataaactt ggaaacataa aaagtaggat gagtaagtct cttacaaatc aaatagagct
    27301 agaccaatat aggttagaaa cagtatttaa taagataaat aactatttag attcgtacac
    27361 aataaaagat aaagttatac aattagacaa aatatatgat aaaataattt ttggaataga
    27421 aaataattta aaattagaag atgaaaaatt agtaaaaatt ggagcattac tccataattt
    27481 aagtccatta gctactatgg atagaggtta tagtataact caaaaaaatg gaaaagtaat
    27541 aaatagtata aaaggattaa aaataaagga tagtatagat atagtattaa aagatggaaa
    27601 tttagaatgt atgatagata aaattgaaaa taaagaggga tagtatgaat ttaacctacg
    27661 aagaagctta taaaagatta gaaagtattt taagtgaatt agaatctaaa aatgctagct
    27721 tggatgaatc tttaagccta tatgaagaag gtataagcct ttataaacat tgtaataagc
    27781 ttttagatga tgctaaattg aaaataagta aatttaatca attaggaata gaagaagact
    27841 ttaaaataga ggaggaataa aattggaatt taaacaatgt ctaaaagaaa aagcaagttt
    27901 tgtagaaaaa gtacttaaag aatatatgcc taaagaagaa gggtatcaaa aaactgttat
    27961 tgaagctatg aactatagtt taagtgctgg aggtaaaaga ttaagaccaa tacttacatt
    28021 ggaggcttgt aaaatagttg gaggaaatga agatgaagct attccttttg ctattgcaat
    28081 tgaaatgata catacatatt cactaataca tgatgatttg ccagctcttg acaatgatga
    28141 tttaagaaga ggaagaccaa caaatcataa agtatatgga gaagctatgg ggattttagc
    28201 aggagatgct ttgttaaact atgcttttga agtaatgctt gcaggctcaa tcaataaaga
    28261 aaatccagaa aagtatctaa aagctataaa tgaaattgcc aaaggtgctg gtatatatgg
    28321 aatgataggt ggtcaagtag tagatgtaga aagcgaaaat aaacaaattg aaaaagaaaa
    28381 attagattat atacatatga ataaaactgc tgcaatgatg gtaggctgta tgagagcagg
    28441 agctactata ggtggagcaa attctgaaca aatggaagaa ataactaaat atgcaaaaaa
    28501 tatcggatta tcattccaga tagtagatga tatactagac atagtaggag atgaagctaa
    28561 gttaggtaaa aaggttggaa gtgatataga aaatcataag tcaacatacc catctctttt
    28621 aggtttagat aaatctaaag aaattgcaca taatttgatt gatgaagcaa agaaaagtat
    28681 agaaaaatta tctgatgatg tagatttttt aaagggattg gctgaatata taatagatag
    28741 agaatactaa aatatacagc taaaatcgag gagggaaaaa tggatttttt ttcggagatt
    28801 tttaacaatg gggctttggg aataagtctg attgcttgtt tcttagcaca atttataaaa
    28861 atatttactg gtaaggaaaa aagaattgag ttatcaagaa tacttatttc aggaggtatg
    28921 cctagttccc atagttcttt tgttacaagt ttagctacag tggtaggtat tgaaaaaggg
    28981 tttaattcta ctgattttgc tataattacc gtacttgcct taattataat gtatgatgca
    29041 gcaggtgtta gacgagctgt tggtaaacaa gctactatat taaatcaaat ggtggcagat
    29101 atacagcatg gaaagcatat agagcagaag aaattaaaag aattaatagg tcatacacca
    29161 ctagaagttt ggtttggagc tttacttgga atagtaacag ctttaatatt gatgtaaatt
    29221 gaaaatttac agaataaagg tgaggataat atgtataaat atttagataa agtaaattct
    29281 ccaaaggata taaaaaatat gagtatagaa gaaatggatt tacttgcaaa ggatataaga
    29341 aagttcttag taaaatctgt ttcaaagaca ggaggacact tagcttctaa tctaggagtg
    29401 gttgagttaa cattagcact tcataaagta tttgatagcc ctaaagataa gattgtatgg
    29461 gatgtagggc atcaatctta tgttcataaa atagttactg gaagaaaaga ttgttttgta
    29521 tctttgagac agtttaatgg actaagtggt tttccaaagg agaatgaaag tcctcatgat
    29581 atttttgata caggacatag cagtacatct atttctatag ctacaggtat tgcctgcgct
    29641 agggatataa aaaaagaaaa ctatagtgtt atatctgtga taggagatgg ttctataaca
    29701 ggaggtatgg cacttgaagc tttaaatcaa ttaggatata tagataccaa tatgatagta
    29761 atccttaatg ataatgagat gtccatagat aaaaatgttg gaggaatgtc taaatatctg
    29821 tcaagtatta taagaaactc aacagttgag aaaatgacag atgaagttga taaaatttta
    29881 aatgttacac aaacaggtga aattttatct aagactgcac atagatttaa agataaacta
    29941 atgtatagct tttctcctca ggactgttca ttttttgatt cattaggtat aagatattat
    30001 ggacctatag atggccataa tactaaagaa ctaatagata tattgagaaa agctaaacac
    30061 aaaaaagggc ctgttctttt acatgtaatt acgaaaaaag gaaaagggta cagatttgca
    30121 gaagaacaac ctgataaata tcatggagta tcaaagtttg atataaagac aggtgttaca
    30181 tctgcaaaag ttaagtctat gtcaattagt gttggagaaa aacttgttga tatggctaat
    30241 aataatgaag atatagtagc aataacagct gcaatgccat ctggtacagg gttaaacctg
    30301 tttgaaagtg catatccaaa aagatattat gatgttggta tagctgaaca acatgccaca
    30361 ggatttgcag caggtcttgc aaaaaatggt atgaaacctt actttgctgt gtactcatca
    30421 tttttgcaaa gggcatatga ccaagttatt catgatgtat gcataacaaa aaagccagtt
    30481 acttttctta tagatagagc tggattagtt ggaaatgatg gagaaactca tcatggtatg
    30541 tttgatttaa gttacttaaa ttctattcca aatattgtag taatggctcc aaaagacaca
    30601 agagagatgg agcttatgat ggatttgtca ttaaaattag attgtccact agcaattaga
    30661 tacccaagag gaagtagtta ttacctagat aagggagaat atggagagat tgtactgggt
    30721 aaatatgagg tattagatga cggtcaagat acagtcatac tttgtattgg aagtatggta
    30781 aaacatgctt tagaggctaa agaaatatta tcaagagaag gaataaatcc gacaatagtt
    30841 aatgctagat tcttaaagcc aattgatgaa ggtatgttaa aagcattatt aaaaaatcat
    30901 aaaaatgtag ttactataga ggataatatt gtaactggag gatttggaag tagaataaac
    30961 aagtttatta tagataatga atataacgta aatatactta atatagcaat accagaagaa
    31021 tttgtaaaac atggcaatat agatgaatta tatgattttg taggattatc gccaaaaagt
    31081 attgctgata aaataaggaa attagttatt gagtaaaaat ctaatgtaga tattgtaaat
    31141 taaaatttat ataaaatata gaatttatca attagaataa atatcaagtg tgttacttca
    31201 agctacttta gttatcatta aagtagcttt taacagaaag gtaattgtat gaaaaaaaga
    31261 atagatttat tattagtaga acaagggtac tttgaaagta gagaaagagc caaaaaagca
    31321 ataatggctg gattggtttt tgttgataat caaaggtgtg ataaggctgg tacagaagta
    31381 aaagaagatt gtagtataga agtaaaagga aatccaatac catatgtcag tagaggtggt
    31441 ttaaagcttg aaaaagcaat gaaaaacttt gatttgacta ttgatggaaa ggtatgtatg
    31501 gatatagggg catccacagg tggttttaca gattgtatgc tcaaaaatgg agcaataaaa
    31561 gtattttcta tagatgttgg ttatggtcaa cttgcatgga aattgagaca agatgatagg
    31621 gttgtgtgta tggaaagaac taatataaga aatgttacaa tagaatatac aaagcagttt
    31681 gctgattttg cgtcaattga tgtgtctttt atatcgttaa agttagtact tccaaaggca
    31741 aaggagttag tacgtcatga tggggaagtt gtcgctttaa ttaaacctca atttgaagca
    31801 ggaagagaaa aagtaggtaa aaaaggagta gttagagaaa aatctacaca tatagaagtt
    31861 ataaaaatga tttctgattt ctcagtcgaa aatggatttg agattttagg gttagatttt
    31921 tctcctatta aaggtcctga aggtaatata gaatatctaa ttcatttgag aaatggaaat
    31981 gaaggatatg aatttgatgg tgagacatac aataacaaaa tagtagaagt tgttgaagct
    32041 tctcataatt tagacaaata ggttaaaagg gggcttagat ttgatcctcg aattatatat
    32101 gaaaaattgt gccttagttg aagaactaag gttgaatatt gataaaaact taaatatact
    32161 tacaggtgaa actggttcag gaaaatctat aataatagat gcacttggac tttgtttagg
    32221 tgataaatat gataggtctt ttttaagaaa aggaacagat aagggacttg ttgaggctgt
    32281 atttttttca gacaatagat atcttaaaaa aatattagaa gaaaatgata taagtatgga
    32341 agatgataat ttattggtaa taactaggtt gatatactca gatggtaaga gtactgctag
    32401 agtaaatgga aggactgtta aagtgtctct tcttaaagaa attgcatcta cacttataga
    32461 tatacatggt caacatcaaa atcaagcttt gttcaataaa gatactcatt taaaatttct
    32521 agaccttttt ggagaaaatg aattggaaga gtttaaaatt gcttataaaa atgtgtacca
    32581 taagtatagt gaagtaaaaa aagctttaaa ttgcttgaca gaaaataaag atgagatgca
    32641 aattcaacga gaaatagatt tattaagatt tcaaataaat gaaattgaag ctgcaaatct
    32701 aaataagaat gaatatgaag atttattaaa acaaagagaa gtttatagaa atagtgaaaa
    32761 gatatataat aatctaaatt cgagttatag caagttacat aatggtgagt ataatgttat
    32821 tgacctaata ggtcttgcat ctaaggaact taatgacata tctaaatatg acagtgtttt
    32881 aagtgaatat agtgatactg tagaaagaat tatgtatgag ttacaagaca tatctggtga
    32941 gataagaaat tacaaagata atatagactt tgaaccttat gagctagagc agattgagct
    33001 tagaatcgac gaaattaata atttaagaag aaaatatgga gacagtatag atgatatatt
    33061 tgagtattat ggaaaaatta aagatagatt agatgaaata ttaaatagag atgaaagagt
    33121 tgaacagtta aaaagcaaac tgatgaatat agaagaagat ttaaaaataa aagccagtaa
    33181 attgactaaa gcaagaatag aagtagcaac tactttagaa aaaatacttc ttgatgaatt
    33241 aaaaagctta aatatgaaaa atgtaatgtt caaggttaat tttgaaaaag gttcatttac
    33301 tttagatgga gtagatgata ttgaatttat gatatctttt aacttaggag aagatattaa
    33361 gcctatatat aaagttgcat caggtggtga gatgtccaga tttatgttag cgtttaaaac
    33421 catattggct gatatagatg atattgatac attagtgttt gatgagatag atactggaat
    33481 aagtgggata gcagcacaga tagttggtga gaaattgagt gatatagcaa agaaaaaaca
    33541 gataatatgt ataactcatt tacctcaaat tgctgcaaat gcagacactc attattgtat
    33601 agagaaagat acttcaaata ataggacgtt taccaatgtt agtaagttaa atgaatctca
    33661 acgaaaaaat gaaattgcca gacttatagc aggtaacaat ataacagaaa aaactattga
    33721 acatgcaagt gaaatcattg aattggctaa aaaatgttag tgaaaaacaa gcattttaat
    33781 aggtgtaaag aatttttata gaaatgcttt acacctattt tttatgatga aattatttta
    33841 aaatatctag tgcctatatt gaagaaaagt aggtttgaaa atggaatgaa ctttgtataa
    33901 atttgtaact ttgaaatttc taaatgctta agtataatat tatttattaa ggtagtagtg
    33961 aggggaggca taaaatgaat aaaaaagata ctatgcactt aatttttata attgtaattt
    34021 ttattttatt atggtatatt gtggtagcag taagtgagat taataaaaat attgaaagtt
    34081 gtagtaacaa attagattat ttgtcacaag agcttatgag tactaagtcg gatattagta
    34141 atactataaa tgaagaaatg tcaaaaagtt atataacaaa aagtattgac ttaaaagtaa
    34201 aaaaaatcga gaaaaaagaa tgtactattg atgtagatgt tcaattaagt aaacttggaa
    34261 aaaatggaaa agtatttttt atgtataaag aagatagtga taaatggaat gaaactgaga
    34321 tgacaaaaca tggggaatta tcatatgcat gtgaaataaa gataaataca ggaagtgaat
    34381 atgattataa agtagttaca agtggagata tatctgaaag ttctgatgta caaaaactga
    34441 ctaaatctga ttatatgcct gaacaaccta tatttaattc tgggataaga gataatagag
    34501 aatactttat tgaaatagta gaaaatagta atctttttaa tcatgagagt gaaaagtcaa
    34561 aaaataaagc ttacgataat atcaggttag aaaaagtaga tattattgta aatgaaggaa
    34621 aaaaagacac tatatacaaa gcccaattag ctgaaggctt agatgatggc aaaactaata
    34681 attcaaatag aaatcaagtg aattatgaag taagttttcc taaaagggat attgatgaca
    34741 taatttatat taaagctaaa cttacataca ataatggagt agaatataca aaggatataa
    34801 ctgaagatat aactatggga ttacaagatt tagaaaaata aatacatatt taaataaata
    34861 ttcaaataga tttgttcaca aatagagtat aaataaaact gtagtagttt tacttatact
    34921 ctattaaaaa attataattt taattccata tatctagccc cttcaattgg attataagta
    34981 taaagttcta tatcataaaa accaaacttt tcatataatc cttgtgcact tttcatgctt
    35041 ggaagagtat ctaatctcat gtgtgtatat ccaatttttt tagcttctac aatgatttct
    35101 tccaatagta tttttcctat ttttaatcct ctaaattgat ttcttacata taatcttttt
    35161 aattcacata ttttaccttc aagttttttt aaagcaacac aacctgccaa attttcatca
    35221 acaaatgcca atatcaatga gcctgaaggt tttttatatt ttcctggaag tgtttttaat
    35281 tcattattaa agtcttgaaa acataagtct atatttagag aatttgaata ttcagtaaat
    35341 agaagtttta cgttttcaag attttctaaa tctttttctt caacaaattg taaagttata
    35401 ttttgagcca ttttatccgc cattatataa acatgtttat ataatgactt cacctccttg
    35461 atatatgtat atttttctta atatcaatta gattttaata ccattacttt ttcaccacca
    35521 tagtcaagct ctccattaaa cttaaatcca aaatctttat aaagcttaat agccatttta
    35581 ttgtcttcga atatactaag atatagttca tcacagttat attcattttt aagatgattt
    35641 acaagaaaat ctaatgatgc ttttccacat cctttacctt gatattttga atcaatcata
    35701 aatctatcaa gccaaactct atcatcttct aaacgaccat acatagcaaa tcctattaaa
    35761 gaattttcat catatattcc aacaggtacc caagctgtta taaatttaga ttcagcaata
    35821 gaaagtgcat tgctttctat gtaatctaac tgacttttat ctacagacaa actaactaaa
    35881 tcgtgccaat tatttatgtc tgcaattctt acatttaatt tcaagatatt accgtccttc
    35941 aaactttttg ttacaatcta atactatcat attatgtatt aaaaatcact tattaagatt
    36001 ttaaataaac tttttgaatg aataaattct caggaaaaag gaattataaa tacataataa
    36061 tacagcatag gagtagatgg atatatgaac aaaataaata aaaatttcaa aaatatttgg
    36121 ataatattag gagctgtatc gatttttacc atggttataa tttttaataa aaattgtaat
    36181 gttaataaag agactcaaat agaagctttt acaattaaga atcatgataa aaaatttgta
    36241 tatcctatgg gaaatataat tggagtaaaa gcaaatacag atggtgtatt ggtcttagga
    36301 tatgaggaag aggatgtagg ctatattgga ggcatacaaa taggtgataa tatagtaaaa
    36361 atagataata aaaggataaa aaatagtcaa gatgtatcag agattttgaa taagattaaa
    36421 aaaagtaaag ttgaagtgac ttttgagaga aaaaataagt ataaaacaga agtaatagag
    36481 actaaaaaag aaaatggcaa gtataaactt ggattatggg taagagataa aatatctgga
    36541 attggaacta tgacttttta cgaccctagt atggaaaagt tcaaagcaat cggtcatgcc
    36601 ataaaagatt cggatactaa tgaactatta aagataaagc aaggctatat atataagcca
    36661 gaacaattaa aaatagtaaa agctagtaat gaaaaagtag gtaaaataaa aggagatttt
    36721 aatgacagca atttaatggg taatttctca aataattcag agctaggtat aagtggtaat
    36781 attacagaaa accataataa agagtttaat gtagcaaata aagaaaagca attaatagaa
    36841 gttggaagac cgcaagatgt aaaaatagga gatgcagtaa ttctttttga ggataaaaac
    36901 aaaaatataa caagctatga tataaaaata gaaagtatag tatatgataa aggaaattat
    36961 agagatatgg taataaaagt agtagatgac aagttattgg aatacacagg aggtatcgta
    37021 caggggatga gtggagctcc aataatacaa aataataaaa ttattggtgc aataactcat
    37081 gtttttagag ataatccgaa aaaaggatat ggtattttta tagatgaaat gataaaattg
    37141 taggtgaggc attaaaaaat tttattattt tatcaattat ctaggaggaa tataattttg
    37201 gagtgtcgaa tatgctttag agtagataat taggaagcaa ttgtgtaaaa agtttagttt
    37261 tctgtaataa gaagatgttt tttaatgggg ggatttttag tggaaaaaat caaaatagtt
    37321 ttagcagatg acaataagga tttttgtcag gtattaaaag agtatttgtc taatgaagat
    37381 gatatcgata tattaggcat agctaaggat ggaattgaag cattagactt agtaaaaaag
    37441 acacaaccgg acttattaat actagatgta ataatgccac atctagatgg attaggtgta
    37501 attgaaaaat tgaatactat ggatatacct aaaatgccaa aaataatagt actatcagca
    37561 gtaggtcaag ataagataac tcaaagcgca ataaatctag gagcagatta ctacatagta
    37621 aagccatttg attttgttgt gtttataaac agaattagag agctagtttc taatagagtt
    37681 acacaagttg agccaaaacc tagaccagtt caagagactc aaatgactag aagtgatttt
    37741 gttaagaatg taggaaatat agagacagaa atcacaaata taatacatga aataggagta
    37801 ccagctcata taaaaggata tttatattta agagaagcaa taaaaatggt tattgataat
    37861 gttgagcttt taggtgcagt aacaaaagag ttatatccaa gtatagctaa aaaattcaat
    37921 acaacaccaa gtagagttga aagagcaata agacatgcaa tagaggttgc atggagtaga
    37981 ggaaaagttg acacaataaa tcaattattt ggatatacgg tacacaatac taaaggaaaa
    38041 ccaactaatt cagaatttat agcaatgatt gctgataaat taagactaga acatagtatg
    38101 gttaaataaa caagacataa aaagtaaggc tttttaatta aggcattggc tataaatgcg
    38161 tattacaagc agcgaaacgg tataaccact agggttatac cgtttcgcta ttttaataaa
    38221 tataaaaatt ttctttatta tttgcttact atatcaatat aataatttta ttatactatg
    38281 gatatagtat gtgtctttac aagttgtaaa ctgacagtgg tttatttttt aatataaata
    38341 ttgactttga tgcaggtaaa ctttgtattt ttaagcgtat tatggaatat gttaaataaa
    38401 aaaatgatga aatatagtat tgtaaatgcc aaagatgcaa aacaaactta aaacatttat
    38461 tttattgtta agtaatgcta taatataatg tgattttaat aatgatagtg gaggtttaaa
    38521 tatgagagtc gaggccccta taaaagtaga tcgaaaaacc aaaaaacttg ctaaaagagt
    38581 tgaaagtggg gaaatagcag ttataaatca tatagacata gatgaagttg ctgcaaactc
    38641 tttagtagaa gctaaaataa aacttgtcat aaatgcggct ccttctataa gtggtaggta
    38701 tcccaataaa ggtccaggga tattaactga aaataatata ctaataattg ataatgttgg
    38761 agaagagtta tttgaagaat taaaagaagg agaaactata gaagttgtag atggaaagat
    38821 atatagaaaa ggaaaatttt taggtgcagg agaagtttta gacaagtatg aggtttcaca
    38881 ccaaataaag gctgcctatg agaatttagc tgtagaattg gatagattta tagataatac
    38941 tatagattat gctaaaaaag aaaaaggatt tatacttgga gaagtagaaa ttccaaaagt
    39001 gaaaactaat tatgctaata aacatgtatt gatagttgta agaggtcagg actacaaaga
    39061 agatttaagt actatgcttt cttatataga agaggtaaag ccagtattgg ttggtgttga
    39121 tggaggagca gacgctttaa ttgagtttgg atatactcca gatgtgattg taggtgatat
    39181 ggatagtgta agtgatgaag ctttaaaaaa ggctagtgaa atagtagtac atgcttatac
    39241 agatggaaga gcaccaggtc ttaagagggt tgaggaatta ggcttagatg cagttgtatt
    39301 tcctgcgcca ggaactagtg aagatatagc tatgcttatc gcctatgagt ataaggctga
    39361 gttaatagta gcagttggta cacattcaaa tatgattgat tttcttgaaa aaggaagaaa
    39421 aggtatggca agtacttttt tagttagact aaaaataggt tcaaagctta tagatgctaa
    39481 aggcgttaat ttgttataca gaagtaaatt aaaaataaag tacatatggg cattaatagc
    39541 aacagcatta tttcctgtgt tggtagttgc atcattgtca ccaggagttc aacagttcat
    39601 acaactaatg caattaaagt ttagagtatt attacagatg taaattttag gaggataaat
    39661 atgcatatta atatgaaata ttatatagtc actatagggg ctatatttat agctttgggt
    39721 ataggcatgt tagtaggatt taatttaaat aataatcaac aattaagtga acaacaagca
    39781 aacataataa atgatttgga tgataagttt aatatcttaa aagaaaaaaa tgataaactt
    39841 gacagtgact tagcaagtgt aaatagagat tatgaagagg cagttaattt tataaataaa
    39901 aatgtagata agattttagc tggttcgctg aatggtaaaa gtataggaat aatatcggct
    39961 aatgaaaatg acgattatac aaaaaatata gaagacataa taaataaatc taatggaagt
    40021 attgcattta atatagtatt aaaagaaaat ataacaaatc cagaaaagtt aaaggaaatg
    40081 tcaactaaac taggtacaga tgtaaaaaat gcaaatgatg cagttaatta tataattgac
    40141 acccttaaaa aggaagatgc aagtgacatt ttaacatatc ttcaagaact agatgtaata
    40201 aagtttaact ctcttggaaa tacatatcta aaatatgatt ctgttgtaat agctggtgga
    40261 aatgatgcaa aagacagtac aaaacagttt gaaaaaatag aaaaatttgt agtttcaaaa
    40321 cttaaatcag agaataaata tttagtagaa gttcaaaata caggagttaa aacttcttat
    40381 gtagaactat attctaaaaa caaagttgca actatagaca atattgatga aggtatagga
    40441 agtatatctc tagctattct tctacaacaa ggtaatatag ttggtaattt tggtagattg
    40501 gatactgcaa cttcattgtt gccatctatt aaatagaaat aattttaagg agagaaaaaa
    40561 tggattctta tataagtata ataataccag cttataatga agaaagtaga ataaaatata
    40621 cattagaaag catattggac attaagcaaa taaatgaaat aatagttgtg gatgatggtt
    40681 caattgacaa tactgctaag gttgtatctg agctagaaaa tgaaaagatt aaatttttta
    40741 agttagataa aaatagaggt aaaggatatg ctttaaatta tggtatcaaa ttagcaatga
    40801 aaaatgcaga tattataggt tttctggatg gagatttagg aagctctgct aaagaagttg
    40861 aaaaattaat agtacctata ttaaatggtg aggcggatgt tacaatagcc aagtttcctc
    40921 ctgcaaagag aaaaggaggt ttgggatttg taaaaggttt agctaaaaat tctgtgtttg
    40981 agatgactgg tgttgagtta gatgcaactt tatcaggtca aagaatattt aaaagagaag
    41041 ttttagaaaa gtttgatgaa ataccttttg gatatggagt agaagttgga atgaccattg
    41101 atatattaaa gtatggattt aaaattaaag aagtattagt aaatatgact cataatgaaa
    41161 caggaagaaa tttaaaaggt tttattcata gagggaaaca gtattatcac ataaaaaagg
    41221 ttttaggaca aaaaaagaaa gaatggaggt aatatcttag tgaaagatta tatactttat
    41281 accattttat tattaactgg attatttgga acctatgctg taattccact atttaaaaat
    41341 ttattgataa atggaaatgt acttagacca aactataaaa aagatatgat accagtaagt
    41401 atgggaattg tatttttgcc tatgattgta atcaatggaa taatacttgc tttctttact
    41461 acagaattta aggatttagc gtatatattt atgtttatgt ttggtatgat atccatgttt
    41521 tttgcaggga tactagatga tgtcattgga aatagagatg taagtggttt aaaggggcat
    41581 tttaaaagtt tatttaaagg aagtcttaca acaggaggtt ttaaagcttt atttggtgga
    41641 tttgtagggc ttatagtttc tgttgctata tcaaaagaca taattgatat aatagtaaat
    41701 acactaataa tagctctatc aactaattta atgaatcttt tggatttaag accaggaagg
    41761 gcaataaaag catatttatt cataatggta attatatttt taacattgac aggatttgtt
    41821 caagttcttc cattacttat agtaccaaat gtacttgcat actttaatta tgatttaaaa
    41881 gcaagggcta tgatgggaga tactggttca aacgtccttg gaatatctat tggaatgctc
    41941 atttcttttg ggtatccatt taacattaga ttaggatggt taatatttgt agttattatg
    42001 catatattca ctgagaaata ctcattaact aagataatag aaaaaaataa actattaaat
    42061 tttatagata aattgggaag gtagtattat gataagcaaa agagtgggga ttgttgaatc
    42121 aatagtaagt caaactgaaa ctctagatga tataagagta aatataaatg gaaaaataca
    42181 aagggcatat aattatccca aaatatctgg aactataaat ataggtgatg aagtggtttt
    42241 aaatacaaca gcagtagaac tgagcttagg tacaggtgga taccattttg taataactaa
    42301 tttaaataat atagaatcta ctttaactga aggtgggcat ataatgaaac ttagatatac
    42361 acctcttcaa ataaaagtgg attctgttga ggaacaagaa agtatttatc atgataaatt
    42421 tgcgaatttt aaaggattag aaggtctttc tgttgtagta gggactcttc atagtatgtt
    42481 aactccattt gcagcttcat ttaaaagaaa taatccaaat aaaaaattag tatatataat
    42541 gactgatgga gcatctcttc caatttattt aagtaaaaat gtggacactt taaaagaaaa
    42601 aaaattaata gatagtacga taactatagg aaatgcgttt ggtggagatt atgaatgtat
    42661 aaatatatat actgcattaa ttacagcaaa agaaatacta aaagcggatg cagtatttgt
    42721 cagtatggga cctggaattg ctgggacagg aacaaaatat ggatttacag ggattgaaca
    42781 aggctctatt cttgatgccg taaaaaaact tgaaggaaga gcaattgcaa ttccaagaat
    42841 tagttttgct gacaaacgag aaagacatca aggtatttca catcatagta tgactgtatt
    42901 taaagaaatt gtaaatgtaa atgtagacat accaataact atatatgatg atgaaaaatt
    42961 gaactttata aaagagcaaa tcaatttaaa tggtttagac aaaaagcata atataattta
    43021 tatagataat aataaaacga aagaggattt agactatttt aatctaaaag taaagagtat
    43081 gggaagaaat tatgaacaag atgaggcatt tttcaaagct gcaagcacag cagcatatta
    43141 cttaatggag gtttaagatg gtattagaag aaaaaactat tagtagtgat agggtttata
    43201 caggtaaagt aattacttta aaggttgata ctgttgaaat tccagggcaa ggatatcaaa
    43261 aaagggagtt agtagaagtt ggtggagcag taggaatagt tgcaatcaca gatgataata
    43321 aagttgtact agttaagcaa tttagaaaac ctatagaaaa gccaatcttt gaaattccag
    43381 ctggtaaact agaaaaaaat gaaagtccaa aagaatgtgc tgaaagagaa ttaaaagaag
    43441 aaactgggta tagtgcaaaa aatattaagc taatacataa atttttcact tcagcaggtt
    43501 tttctaatga gataatgttt gtatatttag caacaggtct tactccagga gagaataatt
    43561 tagatgcaga tgagttttta gatgtctatg aaattgagct tgaagaagca tataacatgg
    43621 tattaaaaaa tgacgttgaa gatgcaaaaa catctattgg attattatta gtaaaagata
    43681 tgtttaaaaa ttaatattta taaaatgaat aaccctttgt cctaaagcat attattttat
    43741 aaaggacaag ggggaattaa attgagaaga acatatagaa aaaatgattt tagagaatta
    43801 aatagacaaa taattattat aggattgttg tttatgatgt ctattgttgt tgggtcttac
    43861 ataaataaaa tattgccagg ttctagtaac aatatattaa acaatataaa tcctgctgta
    43921 gagtattata atctaaatat aagcataaaa gatacagtga tacaaaattt aaaatcagat
    43981 gcaatcttta tgggctcaat agctctatta agtctatttg tagtaacaat tccagcagtg
    44041 ttggtagctt ttgtattaaa agggatgtca attggttata caataaatag ttgtatattg
    44101 gcattaaaat ttaaaagtat aaaaatgatt ttaataatat tatttaaaaa tttgattatt
    44161 attccatgtg caatcatatt agcattaatt agtttaagtt attttaaaga aatggtttat
    44221 gaatttaaaa agaaaaatag aaaaaatatg caatttttaa ttaagaggta tatactaaat
    44281 attataatta taatagcact atcattgggg ttgcaattaa tattaaatac tgtaagtatt
    44341 ggtattatta agttcctagc taaataaagg agtttataat ggatattata gagggatata
    44401 tagattacat aaaagataaa aaaaaattat cagagaacac agtggcatca tactttatgg
    44461 atataaaaaa atatatggaa tatataaatc aaaaaagcat taaactagta gatatagtag
    44521 aaaatgatat tataagttac ttaataaagt tggaaaaaga taatgtatca atagctacaa
    44581 tagcgaggat gatatcctct ataaaatctt ttcatgatta tttattttta aatcatatat
    44641 gtacaaataa tcctgcaaag gatataaaaa aacctaaaat aaaaaaagaa aatataaata
    44701 tacttacaga agaagaaatt gaaagactat taaattttcc aaagttaaca acacctaaat
    44761 tgattaggga caaggctatt tttgaagtat tatatggtac tggtataaaa gtatctgaat
    44821 tagtagagat gaatatagag gatattgacc tagatattga ttatatatat tgtaattcat
    44881 gttgtaaaaa tcaaagagtg ataccacttt gtgatattac aaaactttac ttagaaaagt
    44941 atctaaaaga agcaagacct aaaatggctg tagaaggtga aaaatcatta ttcgtaagtt
    45001 cattaggtca aaggtttaca cgacaaggtc tttggaaagt cataaaaaaa tattctaatc
    45061 tagccaatat agataaaaat ataaatccta caatgcttag acattcattt gctattcatt
    45121 tgttaaatga aggtgctaac attgctgttg taagtaaaat tttaggaaat gttaatttat
    45181 ctagtttgca agtatattta aatcatatag ataaaaacgt gagaagagaa ataaaggaaa
    45241 aacatcctag aaatgatgtt gatgtagaat tacaaaaagc agaagctaaa tagactatat
    45301 agcaaatttt ataatttaaa aggagtatat ttatatgagc agagtaattt ggattgtaat
    45361 tgatagtgtt gggataggag cattacctga tgctgaaaaa tttggtgata gtaaggatgt
    45421 aagtaccctt ggaaatatat ttaaggaata tccagatatt cagattccta atatgagaaa
    45481 tttaggtata ggaaacatag atggtataga tttttttgaa agtataaaag aaccaatagg
    45541 ttgttttgga aagtgtaagg aaatgtctca gggaaaagat actactacag gtcattggga
    45601 aatgactggt ataatagttg acaaaccatt taagacattt gaacatggat tctctaagga
    45661 gataatagaa gagtttgaga aaaaaactgg aagaaaagta gtaggaaata aaccagcttc
    45721 aggaactgta ataatagatg agtatggaga gcatcaaata aaaactggag atgtaatagt
    45781 atatacatca gctgatagtg tatttcagat tgctgctaat gaagaagtaa ttcccctaga
    45841 agaactctat aatatgtgca aaatcgctag ggaaataatg atgggggata atgcagtagc
    45901 tagagtaatt gctagaccat tcataggtaa gaaaaaaggt gagtttgtga ggacttcaaa
    45961 tagaagagat tattcactag acccatttga accaacagtt cttgataata taaaagaatc
    46021 aggacttgat gttttagcag ttggaaaaat agaagatatt tttaatggaa aaggaattac
    46081 agatgctatc cacactaaaa gcaatatgga tggagtggat gaaactttaa attatatgaa
    46141 acaagataat aaaggtctaa tatattcaaa tctcgttgat tttgattcta aatatggtca
    46201 tagaagagac ccagaaggat ataagaaagc tcttgaagag tttgatagta gacttcctga
    46261 aataatggct aatatgagag aagatgatat tttaataatc aatgcagacc atggaaatga
    46321 cccaacatat aaaggtacag accatacaag agaatatata cctgttatga tttatggcaa
    46381 taagattaaa aaaggtttta atttaggagt aaaagatact tttgctgata ttggagcaac
    46441 tgttgcagat atactaaatg taaagttgcc taaacatggg tcaagcttta aagaagattt
    46501 attttaaact ataaaatgat aaaaacttag cttaatagga tggagagatt ggaatgtttg
    46561 aaaagatagc acaaagtagt aaatttataa attctaaaag taatataaaa cctaagattg
    46621 gtttgatatt aggttctgga ctaggtgatt tagcaaatga tatagaagac cctgtcacaa
    46681 ttaaatatag tgaaattcca aacttccctg tatcaacagt agcaggacat gctggacaac
    46741 ttgtaattgg aaaacttgaa ggaaaagaag ttattgctat gcaaggtaga tttcattact
    46801 atgaaggata ttctcagaaa gaagctactt ttccagtaag ggttatgaaa gaattaggag
    46861 tcgagattct tatagtaact aacgctgctg gaggcgtaaa taaagaattt aaagcaggag
    46921 atttgatgct tataagagac catataaact ttagtggaag taatccatta gtaggaaaaa
    46981 atgatgacag atttggtgct agatttccag atatgtcaga tgcatattct tctaaatatg
    47041 tagatgtagt taaaaactgc gctaaagaat gtaatataga tgtcaaagaa ggcgtttata
    47101 tgttttttag tggtccaaac tatgaaacac cagcagaagt gagaatggca caaattttgg
    47161 gagctgatgc tgtaggtatg tctactgttc cagaagtcat agtagcttct cactccaata
    47221 taggagttat tggtatatct tgtataacaa atatggctgc tggcatctta aatcaaccac
    47281 taagtcatga ggaagtaata gaaactactc aaaaagttaa atcagaattt atgaatttag
    47341 ttaaaacaac ggttaaaaat ttataagtta aagcttatta tatatttttt gaggaagttt
    47401 ataataaaat agttaatgtt taatttgaaa agaattaata aaaagctgtc ttgatataga
    47461 aagttatttc taataaagga cagttttttt ataatactac aacatttgaa agtgttttaa
    47521 agtttaatat ttatttaaat tattatagat tagaatggag atttcaatta tgagaatgta
    47581 tgatattata aaaaaaaaga gagataactt agagcttagt aaaaaagaga taaactactt
    47641 tatagaaaac tattcaaagg gagatatacc tgactatcaa gcttctgcac tattaatgtc
    47701 gatttattta aataagatga ataaacagga aactgtacat cttacagaag caatgatgaa
    47761 ctctggagac atgataaacc tgtctgaggt agatggaata aaggtagata agcatagtac
    47821 tggtggtgtt ggagataaga ctaccatagc tcttatacct ttggttgctt catgtggagc
    47881 accagttgca aagatgtcag gtagaggtct tggtcatact ggtggtacta ttgataaatt
    47941 ggagtctata ccaggttttt cgacagaaat ggagataagt aaatttattg attctgtaaa
    48001 taagtctaaa atagcagttt gtggtcaaag tgcaaaggta gcaattgcag ataagaaaat
    48061 atatgctctt agagatgtta cagcaactgt tgataatata tcattgatat catcaagtgt
    48121 aatgtctaaa aagttagctt caggagctga tgcaattgtg ttagatgtaa aaactggtga
    48181 tggagcattt atgaagacat tagaagagtc ttttgagtta gctaaatcta tggttgatat
    48241 aggtactggt atgaatagag atacaatagg gattgtaaca gatatggatg aacctttagg
    48301 ttttgcagtg ggaaactctt tagaagttat agaagcaatt gagacattaa aaggaaatgg
    48361 ccctaaagac cttgttgagc tatgtgaagt attaggagca tatatgttgg ttttatcaaa
    48421 ggtagcacat gattttgaag atggtgttga aaagataaga gaatcaataa aatctggtac
    48481 tgctatacaa aaactcaaag agtttataga gaatcaaggt ggtaataaag atgtagtaga
    48541 tgattattct ttattcaaaa aatcaaagta taaatatcca gttaaatcaa ttaaatcagg
    48601 ttatataagt aaaattaaag ctgaagatgt tggtgtatca gctatgattc ttggagcagg
    48661 tagagaaact aaagatgata ttttagattt atcagcaggg ataattttag aaaagaaagt
    48721 aggagattat gtaaatgaag gtgaagtttt agcttacatg tattataatg atgaacaaaa
    48781 gctttcattg gcaaagggaa gatttattga ttcctactct attgtagatt caaaaataga
    48841 aaaaaataaa ttaatatatg gtattgtaac taaagaagag attaaaaagt tttagaaaat
    48901 tttagaaaag tgattataaa aggcctaaca tgtcaaaaat aatgttaggt tttttatttt
    48961 ttgataaact ataaattata ccaaaaatat atattttaaa acatttttta caatataaat
    49021 aagtttattc tacaagatga ttgaaaatta aagtatctag ttttataata gaaaatggat
    49081 aaatatacat aaggagtaga ttatattatg aactttaaag aaaatagatt gaaaatagca
    49141 gtattaataa ttgttatttt gattatctta gcaggtatat ttgtgtttat acaaataggt
    49201 ccttatgata aaaataacaa aaaagatgta attattgacg ttccaagtgg agcatctgtt
    49261 ggaaaaatat ctgacatatt atatgaaaat aaattaataa agaatgaact attatttaaa
    49321 cttttggtaa aggttagcaa taaagctccg tctataaaat caggaacata cttacttaat
    49381 caatcgtatt caaacaatga tataataagt cttttagttt caggtaaaat atatcaggat
    49441 ggtataaaag tgactattcc agagggagct acatctaaag aaattatagc aatgttagta
    49501 agtaaaaatt taggagataa agctactttt gaaaatttaa taaaaaaacc tcaagaattt
    49561 tatgataaat ttccatattt gaaggaagat ggaataacct ctttagaagg gttcttgtat
    49621 ccagaaactt attattttaa ttcaaagaaa caatcagaag aagatatttt aagcgaaatg
    49681 ttaaaagtat ttgacagtaa atacacggac aagtttaaaa agaaacaaaa agaattaaat
    49741 atgactcttc aagaagtaat ggaaatggca tcaataattg agaaagaagc tgttcttgac
    49801 aaagacagac ctataatagc aagtgtgttt tataatagac ttaaggtagg tatgcctctt
    49861 caatcagatg ctacaataca atatatattt gaagagagaa agaaaattgt aacatatgat
    49921 gatttaaaaa ttgattcacc atataatagt tataaaaata agggattacc tcctacacca
    49981 atatcaaatc ctggtataaa atctattgaa gcagcattat atccagagaa aacagactat
    50041 ttatattttg tagctaagat tgatggtgga aataattata gtacaaacta tcaagaccat
    50101 ttaaaatacg ttaaagaata taaagaagca agggataaac aatcaaaaga tacaaaagca
    50161 acaaataaag aaaatacaaa aaaataaacc atgattataa aaagtctaac agggtaagaa
    50221 taatgttggg cttttttatt gaaaattgtt attcttagat ttataataaa gaaagtgtaa
    50281 atatatataa caatatgaat ttaaaaaata ataaattaat ataaaacttg aatatttatt
    50341 attttaggga gtgtttttat gagtaatata gttaatgatt tggttgaaga ttatataaga
    50401 gtgacattaa aagaaaaaga aggattttta aaagatttag aaatttatgc agaggaaaat
    50461 agtgttccta taatacataa agaagtttca gatttattaa aagtcttgtt aaaggttcaa
    50521 aaaccaaaga gaattttaga agtaggatgt gcaattggtt attcttctat tttttttgca
    50581 agtattatag gaaaagatgc agatataata actgttgaga gaaatgaaaa aatgatagaa
    50641 aaagcaaaag aaaacataaa attagctgga tttgacaata acataactat actagaagga
    50701 gatgcagaag aaaaacttag tcaagtccaa ggtgaatttg accttatatt tatagatgca
    50761 gccaaaggtc aatataagct attttttgat ttagttatag ataaattaaa agatggggga
    50821 cttttagtat ctgataatat tttgtataaa ggtatggttg ctcatgatga ttttgttgta
    50881 agaagaaaaa agactatagt taaaagaatg agaaattatt tagattatat atgtaattgt
    50941 gactatttaa gcacatcatt ggtaccaata ggtgatggtg tagcactaag ttataaagaa
    51001 agtaaaagag gtgatgtaga tggaattaaa taaagtagaa cttttagctc cagcaggtga
    51061 tttggagaga ttgaaaatag ctataacata tggagcagat gccgtttata taggtggaga
    51121 aatttttgga atgaggtctg cagcgaagaa ttttagtaag gaagatatgg cagagggagt
    51181 agcttttgca catgaaagag gcaagaaagt attcgtaaca gtaaatataa tacctcataa
    51241 tgaagatttt ctacaattag aagactattt gttggaacta gaagagatag gaatagatgc
    51301 agttataata gctgacccag gagtgcttag tgtcattaaa aaagtaattc cgaatatgga
    51361 aatacattta agtacacaag caaatacaac caattatcta agtgcaaact tctggtacga
    51421 gcatggaata aaaagagttg tagtggcaag agaattatct tttgatgaaa tatctgagat
    51481 aagagcaaaa acacctttag atatggatat agaagcgttt atgcatggtg ctatgtgtat
    51541 ttcttattct ggtagatgtt taataagcaa ctatatgact ggaagagatg caaacaaagg
    51601 ttcttgtgct caatcttgta gatggcaata tcatttggtg gaagaaaaaa gacctggtga
    51661 gtattttcca atatacgaag atgaaagggg tacattcttc tttaactcaa aagatttatg
    51721 tatgatagag tatataccag agttaatcaa atctggtata acaagtttaa aaatagaagg
    51781 tagaatgaag actgcatact atgtagctac tgttgtaaga gcatacagaa tggcaataga
    51841 tgaattttac agagccccag aaaactggaa gttcaatcca atgtggatgg aagaactaaa
    51901 aaaaggtagt catagacatt ttacatcagg tttttacttg aataaaccaa caacagaaga
    51961 ccaaaattat caatcagcat catatgttag aaactatgat tttataggta tagttagaga
    52021 aacagaagat gaagatggtc ttattgttgt tgagcaaaga aataaaatgt gtgttggtga
    52081 tgaaatagaa gttatgggac catataaaga aactatgttt actaaaattg aagaaatgta
    52141 caatgaagaa ggagaagcta tagaatcagc tcctcatcct agacaaatag tgaagttaaa
    52201 gctatcagta aaagttggta aagattatat gcttagaaaa gttatagaag aaaaggttga
    52261 agaataataa aaaattgata aaaatagtat tagcccctta gaaattgtca ataatgaaat
    52321 tatctaaggg gtgatttcat gtcaaaaaag aagactccat ttttgaaaaa agtgggaaag
    52381 agaagctggt gtatatttac tataatttta ataatttata gtgttttaat ttataggcta
    52441 gttgatatac aagtattaaa aggtgataaa tacaaacaaa gtgttgaatc acaaagcgtt
    52501 gagaaagtcg agttaaatag tggaagagga ataatctatg atagaaacaa caagaagctt
    52561 acagatacaa gtaaatctca ggtattaatt gttgaaaaag aaaaattaaa taataactat
    52621 aaaattttgg aacttattaa gaaagctact aagatgaatg atttagatat atataaagct
    52681 gtacaagagc aattgacacg ccctataata cagattcaga ctaaaaatat cgataagagt
    52741 atgaaaaaag aacttgagaa aaatggaata atggtagaag aaaaaactat gaggtacgca
    52801 aaagatggtt tattatcaca tacaatagga tatataaaag aagatgataa atcaggtcaa
    52861 tcaggaattg aaaagagtat ggatagtgta cttagaaatt caaatgaaaa gtatataagt
    52921 gcttttaaag ctggagatgc aggaaatgaa aaatctctaa acatactaaa gggaagtgta
    52981 aaaactgttg ataataagga taaagataga catctaaaaa ctactattga ttataacatt
    53041 caaaaaaaat tagaacaaat actaaataaa gaagaaaatc ctacagcagc aataatctcg
    53101 gaagcatcta caggtgaaat tcttgcaatg tgctctagac ctaattttga ccaaaatgac
    53161 atatcaaaaa gtttaaaagg aaaaaatggg gaatttgaaa atagagttat aaaagcgaca
    53221 tatccaccag ggtctgtatt taagatggta gttttatttt ctgcacttga aaatggtgtt
    53281 atagatgaga actatactta caactgtaca ggtaagacta aagttgggaa tactaatgaa
    53341 atactgcgat gtaataaaag agatggtcat ggtttccaaa acttaagaca agctttttct
    53401 aattcatgca atcctgcatt tttagatata gctatgaaac ttggaaaaga aaagatactc
    53461 aaatctgctg aaaaattaca tttatttgaa aaggttgata ttggtcttga tgaagaaaaa
    53521 attagagaag ctccaaaaaa catatcaata agaaatttag ctataggtca agaaaatata
    53581 gaatttacac cacttcaaat aaatcaaatg actcaaataa tagctaataa tggaacattc
    53641 aaaccactat acttatataa aagtcttgtg gacaataata tgaatacaat aaaaacttat
    53701 aagtcttcaa aaaaagaaga acttatttct ccttatgtgt gtacacaagt taaagaatat
    53761 atgaagtctg tatctaggat tggtacagct aaagacctaa aagatattga aggtggttgt
    53821 ggagttaaaa ctggtacagc tcaaagtagt ttaaataaaa aagctataga ccacggatgg
    53881 ataacaggat tttatcctga agaaagacct aagtatgtaa taactgtgtt agtagaagga
    53941 actcaaaaag gaaataaatc tgcaacacct atatttaagg aaatatgtga aagtataaaa
    54001 taaattatgt actataaaat ttaaggatat atcgtaactt tgattttaga tagaaaactt
    54061 tgtctaatga tgtacgatat atccttttaa aatatagtat taactttctt gaacaagctc
    54121 tttttctagt tttttaagag cttttttttc gattctagat acatatgacc ttgatatatc
    54181 gagtttttgt gcaatttctc tttgtgtctt atagccataa ggagttaacc catatcttaa
    54241 ctggacaatt tctctttctc taggagtaag aattttgttt agttgttcgt aaagttttcc
    54301 aacttgaact tttagttcaa cttcatcaag aacatagtta gcctctgtgc caagtatatc
    54361 taaaagactt atttcattac acactactct ttatatgtta ttggtattac tggtcttcat
    54421 gtaatttcaa atgaaccatt aattcaaaag aatctagttt ttgttcttta gacttataat
    54481 aaattgcttt atcaattaca gattttagga gtttattttt tccaagggaa tctgaatcat
    54541 gataaagtga taacacagtt ttaatatctt ctattatttt agaattgtct tttttaacag
    54601 tactcaatct attttttact gtcttaattg aatctttaat ttcttcagtt cttgaactta
    54661 tattgttgga cctttcaata aatgtatctt catcatatat tccacgttct aataaatcat
    54721 ataacttatt tttttgttta cctaattctt ttaattcagt ttctaagccc tttaagactc
    54781 gtttttgaaa ttctatatta ctatctttat tatttttatc taagtctaaa tcacatgctt
    54841 ttaattgata tttttttacc gtgtcttcca aagattttaa aatggcagct tcaataaatt
    54901 caaatcgaga acttttattg caaccaggat ggtaacaaat tatatagtcg tggtctgcat
    54961 atggtctata tactaatggt ctaccacaat ttttgcaaat cactattcct gcaagtggat
    55021 tttttatttg attaccatag ctgacatgac cgtttttttt aagtatattt tgagcttttt
    55081 tccatgttat ttcacttatt aaaggttcat gctttccttt tgcttcaatc cattcatctt
    55141 taggtcttag cttaacagtt tttattttat ttggattttt agatttttta tagtcctttt
    55201 tttgccactg aatgtagcca caatatacct tattttttat tatatttgta attgcagaat
    55261 tgtaccaatt agcacctgta gcagttttaa tgcctaagtt atttaggtat tttgatattt
    55321 tactacaacc catatcttca ttaatatata agtcaaagat agtcctaact acatcagcat
    55381 ccttatttgg tacaagtatt ctttcttttc tacctgttgt tacagcatca tatccaaatg
    55441 gagcagaagt tcctatgaaa ttcccttctt caactgattt tattctacct ctttgcattc
    55501 ttcttgatat aagttttaat tcttttcttg ccataaatgc ttcaaattct gaatactcct
    55561 catcaaattc atctgttagg tcgtatgttt ttctaggtgt gatgattttg gttttacttt
    55621 ctttgaatgt ttctaatatg ataccttggt ctttcatgtc tccacgacca agtctgtcta
    55681 agtccattac taagactgca tcatatttat tttcttcaac ttcttttaga agttcaagca
    55741 ttttgggtct atgtattata ctttcaccag aaactaattc ttcttttatc tcaattacat
    55801 ctaagttttg ttcttttgct agttttagta gggttgattt atgtctactt aaagtttcaa
    55861 attcgccttg tttttctgcc tcttcatcag cacgggattt tcttaagtat atggcaacac
    55921 tcataataag taatcacact ccttatgcac tattatatag taaatcaata gagtttaaaa
    55981 tatagaaaat aagcatgtca aaagttaatt taactttcaa catacttatt ttctataata
    56041 ttaatgcctt ataatccttt aatatcattt agattagttt ttgcatattc attatccggg
    56101 ttaagttcaa gcgccttatt gtaagattct ctagctttat taaattctga tttactcata
    56161 aaaataacac ctcttaaatt gtaagcatca ctagcgtcta ttttataagc ttcattattt
    56221 ttatctagat attctaagga tttatcaatt gactttaaag cctcatcata tcgttgtaac
    56281 tctatataaa tatatgattg tgtataatat gcaagtggat ttttatcatc aatttctata
    56341 gcttttttga tatcaatagc agctttatta taatccttat cttcatcttt ttttgtttct
    56401 gacctagcga ttttgacata attagagcta agagctcttt taaagtcttt atgccataat
    56461 aatttatatt cattatcttc gattataaaa tatctatcaa aatttgcagt atcttcttta
    56521 ttttcagtga tatactttat agtatcagtt tcttttattt taacaagatt tgtgtactct
    56581 ttattagaat atttgtattt ctctacttta ttttcttcac caagtttata atcttttagg
    56641 gtttgaagtt taaataaagt atcttgatat tcttcaaatt tttctagact gacactttcc
    56701 ttactgtctt tacttaataa attatatgct acttcgtagt ctttgatttc tatactatca
    56761 aggtactttt tagctacttc tgtagcactt tcttttggag cacatccaac cattgatatc
    56821 ataattaaaa atgatactaa tattgctgtt atttttttac tgattatcat aatataatcc
    56881 cccttatatt gtctctcact aattttattt tatcaccata ttctaccaaa gtcattaatt
    56941 taagaatgat atttttcatt aatattatat taagtaataa taatttttaa aatttatggt
    57001 aacattttcc tgaaaagtat tatataattt aggtgaaaat atttaagaga aggagatatt
    57061 atgaaaatta aaaaattatt aacaataggg ttatctttat ctatatttat tgctagttgc
    57121 cctatatctg caaatgctct tgataaaatt gaatcaatta aaggtgcaga taaatacgaa
    57181 actgctggta taattgcaga caaacaaaac tatactacag ctatattaat taatgcagac
    57241 agtacaatgg ctgatggatt aagtgcaagt ggacttgcag gagctataaa cgctccaatt
    57301 cttttgacta agaaaaataa cataccaaat gctacactta aaagattaga aaaggcaaaa
    57361 aaagtatata taattggagg agaaaactct attaataaat atactgaaac tgttctaaaa
    57421 ggcaaaggaa tagagattaa aagacttcaa ggtagtgata gaattaagac aagctataat
    57481 gttgcaaaag aaataaattc aattaacaaa gtaaataaag tcatattgac taatgctttt
    57541 aaaggagagc ctgatgcaat gagtgctgca cctgtagcag ttagagataa agcagctata
    57601 gtattaactg atggaaaaag tgttccattt aatacaactg gtgtagaaag ttatgtaata
    57661 ggtggtactt catcaatgaa tgacaaacta gttaaggata ctaattcaac tagattaggt
    57721 gggacagata gatatgatac taacaaaaag ataataaata aattttatag tggagtaaaa
    57781 gaattttata tagcaagtgg tactgattta gtgtatgctc ttgttggttc taccattgct
    57841 aaaaataatg ctattgtgtt agttggtaat gatagtaata aaagtgtatt gaaaagtaca
    57901 attaaattaa ctgctatagg aaatttgagt gatagtatat tacagcagtg tttaaatgta
    57961 acaaaaaata taggcgattc taatactgga agagaatata cagtagaaga tgctgctaat
    58021 gcagtgaaaa aactattagg tgtgaagaaa ttttattacg aacctaatta ttcagaagat
    58081 gaagatttaa tatttctact agataaaaat tttgattttt caggaaaaga gtactatgta
    58141 tttgcttaca ttaaacaggg tgcagagggt gatgttagat actgtgtagc taaaaatgat
    58201 atgagcaaag tttatacata tgatgttaat ggtaatatga aagtatgtaa tccagatgat
    58261 tataaaaata aaggaagtat aacagaggaa caagcaaaac agatagcatt aaaagaatgt
    58321 gctaagagac ataaagtcag tatcaataac ttagtagttg atatgataga ttttagagat
    58381 gatatatata gtggtaatgt ctatgctgtt aaaatcaatt caaaaaatag taatggtact
    58441 tttggttggt tttttataga tatagaaact gggaaaattt ataattaaaa ttagaacttt
    58501 taaacaataa ttatataatt taccataata tgttataata tttttagcaa gaagatttaa
    58561 cttacaatct atagagtgga gttcatgtac aaaagattat cctcccaacg attcgaaggg
    58621 aggtgagttc atggataact ttttgtttaa tgttttagct agtttaacag ctagtgtggt
    58681 agtttactta atcagtaaac tattcaaaaa agcaaaaagc cactctcgc
//
LOCUS       DAEPLF010000005        55612 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEPLF010000005 DAEPLF010000000
VERSION     DAEPLF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55612
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.005"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            673..1653
                     /locus_tag="KR314_000285"
     CDS             673..1653
                     /locus_tag="KR314_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0326695.1"
                     /translation="MNLKLIWINILNPVFIKNEIKRKKIQIIIFFFINFGLTYSLGLL
                     LYYNKFIDPENFASFMMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVL
                     KLLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIV
                     PTSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKL
                     GVIILGMCWSLWHLPLIFTLYTPEAPILGVILRSIHIVGISIFLGYLYIKTKNIWFCA
                     IIHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
     gene            complement(1752..2714)
                     /locus_tag="KR314_000286"
     CDS             complement(1752..2714)
                     /locus_tag="KR314_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0326696.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIITVMAAFYIFEYYLSTILMEI
                     IRKILIATGDINLKTNYFSEYANWLNDVGLQVVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(3034..3984)
                     /gene="hpdA"
                     /locus_tag="KR314_000287"
     CDS             complement(3034..3984)
                     /gene="hpdA"
                     /locus_tag="KR314_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBH0326697.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFKDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSAENISDIISFMHKNNLIEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDIDEEHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(3977..4234)
                     /gene="hpdC"
                     /locus_tag="KR314_000288"
     CDS             complement(3977..4234)
                     /gene="hpdC"
                     /locus_tag="KR314_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBH0326698.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDSACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            complement(4238..6946)
                     /gene="hpdB"
                     /locus_tag="KR314_000289"
     CDS             complement(4238..6946)
                     /gene="hpdB"
                     /locus_tag="KR314_000289"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBH0326699.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            complement(7243..8259)
                     /gene="tsaD"
                     /locus_tag="KR314_000290"
     CDS             complement(7243..8259)
                     /gene="tsaD"
                     /locus_tag="KR314_000290"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBH0326700.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSNKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            complement(8265..8741)
                     /gene="rimI"
                     /locus_tag="KR314_000291"
     CDS             complement(8265..8741)
                     /gene="rimI"
                     /locus_tag="KR314_000291"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBH0326701.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKCIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            complement(8731..9447)
                     /gene="tsaB"
                     /locus_tag="KR314_000292"
     CDS             complement(8731..9447)
                     /gene="tsaB"
                     /locus_tag="KR314_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBH0326702.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            complement(9447..9899)
                     /gene="tsaE"
                     /locus_tag="KR314_000293"
     CDS             complement(9447..9899)
                     /gene="tsaE"
                     /locus_tag="KR314_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBH0326703.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            complement(10038..11078)
                     /locus_tag="KR314_000294"
     CDS             complement(10038..11078)
                     /locus_tag="KR314_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBH0326704.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWEDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            complement(11407..12012)
                     /locus_tag="KR314_000295"
     CDS             complement(11407..12012)
                     /locus_tag="KR314_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0326705.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(12137..12254)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(12314..13480)
                     /locus_tag="KR314_000296"
     CDS             complement(12314..13480)
                     /locus_tag="KR314_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0326706.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(13628..15769)
                     /locus_tag="KR314_000297"
     CDS             complement(13628..15769)
                     /locus_tag="KR314_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBH0326707.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYNEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(15913..17020)
                     /gene="prfB"
                     /locus_tag="KR314_000298"
     CDS             complement(join(15913..16944,16946..17020))
                     /gene="prfB"
                     /locus_tag="KR314_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBH0326708.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(17035..19710)
                     /gene="secA"
                     /locus_tag="KR314_000299"
     CDS             complement(17035..19710)
                     /gene="secA"
                     /locus_tag="KR314_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBH0326709.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMVREDLPDSVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(19842..20393)
                     /gene="raiA"
                     /locus_tag="KR314_000300"
     CDS             complement(19842..20393)
                     /gene="raiA"
                     /locus_tag="KR314_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBH0326710.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDSEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(20946..21620)
                     /locus_tag="KR314_000301"
     CDS             complement(20946..21620)
                     /locus_tag="KR314_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBH0326711.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(21623..22675)
                     /locus_tag="KR314_000302"
     CDS             complement(21623..22675)
                     /locus_tag="KR314_000302"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBH0326712.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISKKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKTPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSNTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(22723..23682)
                     /locus_tag="KR314_000303"
     CDS             complement(22723..23682)
                     /locus_tag="KR314_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBH0326713.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIVDFTFSVV
                     TKEESPAVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(23778..24296)
                     /locus_tag="KR314_000304"
     CDS             complement(23778..24296)
                     /locus_tag="KR314_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBH0326714.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNEKEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            complement(24307..25641)
                     /gene="celB"
                     /locus_tag="KR314_000305"
     CDS             complement(24307..25641)
                     /gene="celB"
                     /locus_tag="KR314_000305"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBH0326715.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIVTPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(25680..26006)
                     /locus_tag="KR314_000306"
     CDS             complement(25680..26006)
                     /locus_tag="KR314_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0326716.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(26031..26339)
                     /locus_tag="KR314_000307"
     CDS             complement(26031..26339)
                     /locus_tag="KR314_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0326717.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(26480..28393)
                     /locus_tag="KR314_000308"
     CDS             complement(26480..28393)
                     /locus_tag="KR314_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0326718.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(28725..29507)
                     /locus_tag="KR314_000309"
     CDS             complement(28725..29507)
                     /locus_tag="KR314_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBH0326719.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(29510..31729)
                     /locus_tag="KR314_000310"
     CDS             complement(29510..31729)
                     /locus_tag="KR314_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBH0326720.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(31993..33090)
                     /locus_tag="KR314_000311"
     CDS             complement(31993..33090)
                     /locus_tag="KR314_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326721.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSNIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(33406..34599)
                     /locus_tag="KR314_000312"
     CDS             complement(33406..34599)
                     /locus_tag="KR314_000312"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBH0326722.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(34736..34839)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            35028..35537
                     /gene="yyaC"
                     /locus_tag="KR314_000313"
     CDS             35028..35537
                     /gene="yyaC"
                     /locus_tag="KR314_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBH0326723.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(35648..36076)
                     /gene="fabZ"
                     /locus_tag="KR314_000314"
     CDS             complement(35648..36076)
                     /gene="fabZ"
                     /locus_tag="KR314_000314"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBH0326724.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEIEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(36092..37111)
                     /locus_tag="KR314_000315"
     CDS             complement(36092..37111)
                     /locus_tag="KR314_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBH0326725.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(37308..37568)
                     /gene="spoIIID"
                     /locus_tag="KR314_000316"
     CDS             complement(37308..37568)
                     /gene="spoIIID"
                     /locus_tag="KR314_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBH0326726.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(37666..38334)
                     /locus_tag="KR314_000317"
     CDS             complement(37666..38334)
                     /locus_tag="KR314_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBH0326727.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(38400..39464)
                     /gene="spoIID"
                     /locus_tag="KR314_000318"
     CDS             complement(38400..39464)
                     /gene="spoIID"
                     /locus_tag="KR314_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBH0326728.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKSTVSYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(39551..40804)
                     /gene="murA"
                     /locus_tag="KR314_000319"
     CDS             complement(39551..40804)
                     /gene="murA"
                     /locus_tag="KR314_000319"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBH0326729.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(40825..41562)
                     /locus_tag="KR314_000320"
     CDS             complement(40825..41562)
                     /locus_tag="KR314_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBH0326730.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            complement(42060..43355)
                     /locus_tag="KR314_000321"
     CDS             complement(42060..43355)
                     /locus_tag="KR314_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBH0326731.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKMQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKRAVDFSANWLNVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSITPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSNSVSLEEYRNIDNTMRNKSEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(43808..45640)
                     /gene="glmS"
                     /locus_tag="KR314_000322"
     CDS             complement(43808..45640)
                     /gene="glmS"
                     /locus_tag="KR314_000322"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBH0326732.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEEEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            complement(45907..47253)
                     /locus_tag="KR314_000323"
     CDS             complement(45907..47253)
                     /locus_tag="KR314_000323"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBH0326733.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILNGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            complement(47423..47980)
                     /locus_tag="KR314_000324"
     CDS             complement(47423..47980)
                     /locus_tag="KR314_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBH0326734.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            complement(47981..48733)
                     /locus_tag="KR314_000325"
     CDS             complement(47981..48733)
                     /locus_tag="KR314_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBH0326735.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            complement(48733..49812)
                     /locus_tag="KR314_000326"
     CDS             complement(48733..49812)
                     /locus_tag="KR314_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBH0326736.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            complement(49841..50056)
                     /locus_tag="KR314_000327"
     CDS             complement(49841..50056)
                     /locus_tag="KR314_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0326737.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            complement(50099..50776)
                     /locus_tag="KR314_000328"
     CDS             complement(50099..50776)
                     /locus_tag="KR314_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0326738.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            complement(50766..51845)
                     /gene="buk"
                     /locus_tag="KR314_000329"
     CDS             complement(50766..51845)
                     /gene="buk"
                     /locus_tag="KR314_000329"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBH0326739.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            complement(51867..52769)
                     /gene="ptb"
                     /locus_tag="KR314_000330"
     CDS             complement(51867..52769)
                     /gene="ptb"
                     /locus_tag="KR314_000330"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBH0326740.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            complement(53073..54254)
                     /locus_tag="KR314_000331"
     CDS             complement(53073..54254)
                     /locus_tag="KR314_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326741.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNIDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            complement(54251..55090)
                     /locus_tag="KR314_000332"
     CDS             complement(54251..55090)
                     /locus_tag="KR314_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBH0326742.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRSGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
BASE COUNT        16904 a        10416 c         5857 g        22435 t
ORIGIN      
        1 atattactaa taattatatt tactctttaa taataaataa aattttaatt ttaaataaat
       61 ttatttttga tatttatatt tatttttttg atattaacat tacaaattca tcctaatttt
      121 tataaataaa ataataaact aactttttaa taaatttttt gtattatttt gtatatagga
      181 aattattgaa atcataaaaa atcaacgtta tattataatc atatagtaaa ttaaaatcat
      241 ttgatttatt taattattat ttattaacca gacgaatgta ataattatta aatatacaat
      301 attaaaatca aagagaataa taaatgtaat attaaaactc aataataaat ttatattcat
      361 aagtttaatt tcttcattaa ttttatcaac tatgcctcta ctgcctatta atttaatgca
      421 ataaataata aatcttttac aaaaagataa taaaaatata gaaaaatatt ttttattgtc
      481 cttttgctta tggtaaacca cattctgcct gcaatataac taaatatatg ggtatatagt
      541 aaaattaatt ttattattta tatataattt tataagttac ttttcaataa tattgactgt
      601 catgaattta taatattcaa taaaatattt taatttagtt aagaatctat attgtaaagg
      661 agtattaaat taatgaactt aaaattaata tggataaata ttctaaaccc agtatttatt
      721 aaaaatgaaa taaaaagaaa aaaaatacaa attattatct ttttctttat aaactttggt
      781 ttaacttatt cattaggttt actgttatat tataataaat ttattgatcc agaaaacttt
      841 gcatcattca tgatgatatt acctctatct tcagttgcta ttgcaaagtt ttatactgaa
      901 ggattaaata atgatatgta tgaattttat tcactaatca tattattttt tcttatttat
      961 tcattattaa ttgttcttaa gttattaaat ctaataaata ataatgaatt tataattata
     1021 aacctaatat taacttttat aagtagctta tcaattataa tatattcatc aaataaaaaa
     1081 aatttatatc ctgtaaaaaa tattaaacta ggttctttat taattattta ctttatgatt
     1141 tctgtaattg ttccaacatc atttatacta atactacaag gggctcaacc taattatagt
     1201 ggtatcataa aatttatatt tactccaata acatcattaa ctactattta tatttttttt
     1261 agtgaagaat atgcctggag aggtttttta cagaatattt tttttgataa atttggtaaa
     1321 aaattaggag tcataatatt aggtatgtgt tggagcttgt ggcacttacc actaatattt
     1381 acattatata cacctgaagc gccaatatta ggagtaatat tacgttcaat acatatagtt
     1441 ggaatttcaa tatttttagg atatctttat ataaaaacta agaatatctg gttttgtgct
     1501 ataattcatg ttttaaataa tagtgctttt cttatcacaa attctaaaga atcaactatc
     1561 acttaccatg atatactaat agtatctatg gttgttttaa ttttttatgt tccatttcta
     1621 tttacaaaag aatataaaaa taacttggag taaatagaat atccaagtta tttttatatt
     1681 tttgtaaatt tccaacaatc ctacagaata ctttaagttt agaagatatt attaaaattt
     1741 attataatca actaattctt gatattagtc acatttactt tatcactaac aaaatcatac
     1801 ttagaatctc tatagtaata ccatacaaat ataaagctaa gtagattaac aaaagtagtc
     1861 aaaattccac cttcaatacc aaacatccct ccatttaata gattaccatg agtgatttgt
     1921 gtagttataa tccctggaac ctgaagtcca cttacaggca taccatatat aatcccctga
     1981 aatacattcc atgtaaaatg ataacctata cacatccata tattcgacga ctttataaac
     2041 atatatgaaa aaagaatccc aataattata atattaatta cagacaaaaa agtaacatca
     2101 ggattccata tatgaatcaa cccaaaaata acagatggaa caatcattat aaaataaata
     2161 tttttacatc ttcgtaaaac tgccataaaa aaaccacgat ttaaaatttc ttctgcaaaa
     2221 gcaactaaaa taaagacaag tatccaagaa actgttaaag gtgaaatttt tggtacccat
     2281 gaagtaacat gaccaccacc aattgttatt gttataataa agattactgt acaattaaat
     2341 attcctaata acattcctac atatccttct tttttcttaa ttctataaga atgtaatccc
     2401 atttcactaa taggattttt cataataaat ctccatacaa ttataggaat aataatcata
     2461 agtgaatctg ttagaatttg aaatacaacc tgtaacccaa cgtcatttaa ccaattagca
     2521 tattccgaaa aatagtttgt ttttaaatta atgtcacctg tagcaatcag aatttttctt
     2581 attatttcca taagtatagt gcttaaataa tactcaaata tgtaaaaggc agccataaca
     2641 gttataataa tccaccctga acgtactttt ttgtctttat tcttaataat caagttactc
     2701 catgatttgt tcatatagat gtcttcctcc taactattat ttttagttgt ttctaaaata
     2761 tattctatta aatttcttca tagtagtatt ttaccacaaa acttctgctt caataaagta
     2821 aacgtaatat attctctaac tgtaatttaa actataaaaa atatataaag tataaattta
     2881 aagtaatatt tatggacata aagcaatatg aacaatatat aaaacatata aaactttact
     2941 taattatttc tacataaaaa ccacataaaa aaggatgtaa ctctagcttt tagtgtaaca
     3001 tcctttttta attaaaaaaa cttgtatttg attttaaaaa gctgtctcat gaccaacata
     3061 gcaagctata ccattatcca aaaatatatc ttgtaattct tcaagatgct cttcatcaat
     3121 atctccctta tcagaatact catactcctt acctaattga atccactttg attctcctag
     3181 cctatgaaat ggtaataagt taatctctat taaattattt ttgtgcataa aagatataat
     3241 atcacttata ttttcagcac tatcattaaa cccagagata actggtactc taagtactaa
     3301 ccttccattc cagtcagaat ttgcaaggtt tgatatattt tctaatatta aatcattata
     3361 tacacctgtt tgttctttgt gtttttctct atccatatgc tttatatcaa taaatgcaaa
     3421 atcaatatct ttaaatattt tattaaaaac ttcatttgat acacatgcac ttgtttcaat
     3481 ggcagtgtgt atatttacct catgacactt taataatact tcatgtaaaa attcatgctg
     3541 aagtagtggc tcacctccac taaatgttac tcctccatta cttctccaat tgttagaatc
     3601 acgtttaatt acctgcacaa gttcatctac agtataaggt tttgcacata atttaaacgc
     3661 attataataa catgagttga cgcattcaaa actttcacag tccttacaga tgttccaatc
     3721 tataactggc ttattatcaa gattaaagct caatgcacca tttttacact taccatgaca
     3781 tacagtacat ccattttcat attgacaaga taattcacta aacatcatat gtggtcttac
     3841 agtccaactt tctggatttg cacaccattt acaactcagt ggacatccat ttaaaaatac
     3901 agttgttcta caacctggac catcatgaac tgaaaagctt tgtacatcga aaatcatgcc
     3961 ttctaattgc ttttgactac tcattaaaca catgtccttc acaagtaatt gcattcaatg
     4021 ttgaatatac ccagtcttct ttctctaggc caacacattt gcctatacct tcatcattag
     4081 cttcaacaaa atttttacaa tttttacatt ttggcattac ttttatctct ggacatgctg
     4141 aatcatctaa attaatcatt tgttttgata atctacaaat tcctttggtt gcatctactg
     4201 cacaaaaatt catacaatca ctatgctttc tcatatttta caccccttca tactctgttc
     4261 tagcaattac ttcatcttgt attggtttac ctaattcaca ccaatattga gtaaacccag
     4321 caacacgtac cattaattga cgataattgt caggattttt ttgtgcatct tttaatgttt
     4381 ctgaatctac aacattatat tgaatatgga acccaccttt tcttaaataa cttcttgtta
     4441 aatctaataa ttttttagtt ccttgttgtc cttttatagt agttggatga agttttaaat
     4501 tcatctgaga attttgaact actgcttggt cccaaatagt tgcagattca aataatgcat
     4561 atactccatt tttatctgtt cctgcatatg cagagactga accatctgaa tatgttgttc
     4621 cacttagtct accatctgga gtagccaagg tagcagctcc ctgtggtcca tgtgtagaaa
     4681 cagatatttg gcaagggtac atcttcttag catataaaga ttgtgcttct tcacatactt
     4741 ttgataacca tacctcatag tttttaagaa tattatctgc atacaaatca ttatttccat
     4801 atttaggtgc atcaagacag gccttgtaaa tagcatcata tttaccagtc ttatctactt
     4861 ttacttggtc tgccatagaa taatttccta cttctaatgc atctttaaat ccaaaattat
     4921 ttaatattgc atccttcatt tcctctattg taaatgcttt gtcatcatat acaagtttct
     4981 taagtgctgc aaatgaattg accatagtaa cagtaccaca agtttctacg tttaatgttg
     5041 cattataacg atatcccata tttcctatat gttgaccttt gtctaaacag tctggcttta
     5101 gtagtgaatt tattatagaa gtatcaaatt ttctccatat atctaactca atgttatttg
     5161 ctctttctaa aacatttatt gcttgtttat aataatcttt aaatacttct atgacttctt
     5221 cataagtatc taatggctta ttatgaggct caaatacttg tatattcata cgatggtctt
     5281 taccattcat aagaacaagt tctaaaattt tgggattagc tataaagtga actccagagc
     5341 cagcagattg acctgctcca cctgcaatag agtacgtttt tccgtttaaa gttaactgtt
     5401 tccaacaccc tggagacgtt tccaaacatc cacctaatgc aaatgctctt gcttcttcaa
     5461 cagtcatacc ttcatcagca aattgtttca tcataaatgt tgttccattt gagttgttta
     5521 cccaggctgg ataaccagaa ccaagctttg tacattcagc agctttcatt aagaaatctt
     5581 ctggaagctt ttcatcatat agcatagtta aactaggttg aggagttcta catctcatac
     5641 ttgcttctaa taataactct tctagttcat tagctcctgt agaaccatct tcttttaatc
     5701 cacctataga taaagtatta aatgtattac ctgatagtac tcctccattt acgcccgctg
     5761 aagcaaaaca gtcaatacaa gtgattttta ttctataaag ctctaataat tcgataacct
     5821 cttcttttgt aattagtcct ttttctatat cttgttcata ccaaggatat aaaatttgac
     5881 caaaacgtcc tatagacata ccagatatag catcctcatt tactgatgct atatgaattg
     5941 tataagtcaa ctgaagagct tccctaaatg tacgaggttg tttatgtgca atatgttcta
     6001 atatctccac taaatctgag tattcttttt tagcttctaa atctgtctct atactttcta
     6061 aatattttgc atgttttgca taatttaaaa tccaagtttg taatccttca ataacttgaa
     6121 taacagcctc ataaaaatat agacggtcca taccaataag cccatctcca gatgcatttc
     6181 ctgcaactgc ctttttacat tcttttgcca tttctataat tccatctaat ccataattta
     6241 aaggataaaa ataatttata acttctctac cttgaggaag tgtataccct gaatcaaaca
     6301 tgcatataag tgtactcatt aggttttctt ttaaatcata atctggcatc attttttcat
     6361 agtgaaaacc taaatcttca acagactttc caacccattc ctttgccatt tttacaagta
     6421 caggtacttc ttcttttctc attccgaatt taccagctat tgaaacgaca tttccaaaac
     6481 tttcagtaac atttcctcca ccagacccaa attttgtaag ttcatcagct gtgttactgg
     6541 ctaatttctt tgcctcttct ctcatttgtt ctccttgagc aacaaagaaa ctttcactta
     6601 cccatggcat tggaaaagaa cctctataat ggcgtgtctt ttgcattact attttctcac
     6661 caggtattat atttggtgtc atatgtgaaa aagcagcttt aagagcttta gctcttctaa
     6721 ctactggtat atcaccatct gattttctat attctctatt ataccaatat ggaaattcca
     6781 tatcagcagt agataatgta ttataatata catctaataa gtcttctgct cttttacttg
     6841 cttttttttc attaaattca cttaaatttt ctttattttg aaaatttgat ttaatatttt
     6901 ttgcttccaa aatacttctt attttgtctt ctttactttg actcatttct tcccctcctt
     6961 aatctttcta taattagtat aaaacttttt aagaatgaaa aaaaagggta tattacccac
     7021 tttttaaaat aatctttcta tagaaaaaat taaacttgaa gcaaatctaa gccaaagaca
     7081 atctgtatat cctaaaattt gttgcaattc attggaatat aaagattata aatagtttta
     7141 cttcaagttt taatatatac tatatttatt tttattatat ttaatttcac ttctacttac
     7201 ttttttgaaa caatactttt atatttacaa attcatctaa ctctactgat ttatctttaa
     7261 atttggaact gcatttaaac tcatatcatg cgttttccca tttataaaat tataatatcc
     7321 tgcacaccct atcatagctg cattatctgt gcataaaatt agaggaggat acttaacagt
     7381 aataccattg tctttagcaa gctctgttat tttagctcta agccccgaat tagaagcaac
     7441 cccaccagaa agagttatga tattgtatcc tttgtctttt actgctttta gtgctttatt
     7501 agataaaacc tctactacag cctcttggaa agatgctgct acatcttcaa ctactatttc
     7561 ttcatttttc attctttttc catttagata atttaataca gaagatttta atccactaaa
     7621 actaaaatca tagctatctt cttctaaata ggctcttgga aattctatag catgtttatt
     7681 tccattcttg gccagattat caataatagg acctcctgga tagcctaaat tcatagctct
     7741 tgaaatctta tcaaatgctt ctccagatgc atcatctctt gtttttccta aaatttcata
     7801 cttgccataa tcttttactt caaccaagtg agtatgacct ccagatacta ttaatgttat
     7861 aaaaggaggt tttaaatcct tatgctctat atagtttgca cttagatgcc cttctatatg
     7921 atttactcct acaagtggaa tattcaaagt atatgcaagt gctttagcat aagatagacc
     7981 aaccaataaa gcacctacta gcccaggtcc atatgtaact gcgatatggt ctatatcatt
     8041 aaaacctata tttgctttgt ctaaagcttc ttgaacaact atatcaatat tttccacatg
     8101 ttttcttgag gccacttctg gaactacacc accaaatttt ttatgtgtct cgatttgagt
     8161 agagattatg tttgaaagta cttctctacc attttttaaa actgatgcag ctgtttcgtc
     8221 acaactactc tctatagcta atgttattat atcactcata aactctacac ctcttttaat
     8281 tggttccaca ttataattgc atcttcttta ttatcactgt agtattcttt tcttattcca
     8341 cccattttaa atccatattt tctatataaa ttttgtgcta ttttatttga tgctctaact
     8401 tctaatgtca tagatgcaat attattatct ttacataaat caactatgca ttttatcaac
     8461 ttgtccccta tttttttccc tctatagtca ctatgaacag ccacattatt tatatgccct
     8521 tcatcaagta taagccaaat tcccacatat ccgacaattt ttccatttaa cttagctact
     8581 atataccttg ctacttcatt agacaactct tttctaaaag actcttttga ccaataatct
     8641 tcaaaacaat ttttttctac ttcaaaaact tcatctatat cttctgtagt catttcttct
     8701 atttttatat catctactat tagttttgta ttatcttcca ttatttagcc tcttcatttt
     8761 ttcatcatat tgtacttcag cttgagattt tctgatatac ataggattta tcgtataaca
     8821 agtatgtaca tctatatttt tattatattt atttaatgct aaagagcata agcttcctgc
     8881 gttagataca ttattagctg gtgaaggtat ttttatattt tttatattct ctaacttgtc
     8941 tttatatttg tatacagctt ctccaactat tataaattct tcattagttg atacaatttc
     9001 atctactaaa gcatcaaact ctacaacatc aacaccatct aattctatca tctctccatt
     9061 ttcaaacttg tatttagctg aataaacttg atttttctgc gcatctaata tacaacatat
     9121 ctttctatca cagaaattta tgttatttgc tagagattcc aaagaattta ctgctattat
     9181 tggtatatta tttacatggg ccatagcttt tactgtagcc atacctattc taagtcctgt
     9241 aaacgaacct ggacctatac atacagctaa taaatccata tcttttatac ttaaatcact
     9301 catactcaac atattttcta tcattggcat aagcttttgt gaatgagttt ttttgttgtt
     9361 tactgtaaac tcacatatta agttatcatc ttcaacaact gctacacttg ctgccataga
     9421 agacgtatcc atacctagta ttttcattat ttaatcaact cctcaactat ctctttataa
     9481 aactctcctt ttggagttaa tatcatttct cttccttcat ctttatatct caattctata
     9541 ttcaaatatt cttttggaag tatatcttct attagattag cccattctat tatacagatt
     9601 ccatttgaat ttacatattc atcataacca atatcataca tttcatcact actacctatt
     9661 ctataaacat caaaatgata caaaggtatt tttccttcat attcatttat tatagtaaaa
     9721 gtaggacttg ttatataatc ttctatccct aatgaatctg ccaagctttg tgtcattgta
     9781 gttttaccag ctcctaaatc tcctactaaa cagataacac tgccttcttt taaaagttta
     9841 cctaatttat atcctatttc tctagtttta ttttcatttt ctaaatatat ttttgccatc
     9901 aattcatctc cattttgata taatcgtcat gtgaatttat atcactgctt aattaattat
     9961 agtataagcc tagattatta taaagtactt gtctttatct ttttttatct ttcactattg
    10021 atggctgtat ctacacctta tatacaatca tcaatagcaa tttttacata ctcttttgct
    10081 ttatggtttt gcttcaatgc ttctttacat attctctcag attgctcttt tacatactta
    10141 agagacttat agttctcttt tactgcttca atacacattt tttctgtttg attttttata
    10201 tacataagca tttttccatt ttgtcttaca atctctatac atatatcttc tgtatactta
    10261 tctatgtaac ttagtacatc tttaatatta tattttttat ttaaatatct tatagcattt
    10321 atacatattt cttctgtctt attgttgaca aattgtagac ttcttccatc ttgtttgaca
    10381 gcttctatac atattttatc agtttgttta tttacatatt gtaatgccat accattttgt
    10441 cttacggatt ctatacacat tttttccgtt tgatttttta catattttaa tgctttataa
    10501 ttttgcttaa tggcctctat acataattct tcactctgtt ctctaacgta tgcaagacac
    10561 ctgccatctt ttttaattgc ttctaaacat atccttcttg tttgagtctc taaagccttt
    10621 agtatactta caacatctat attggaagaa tgacttattg cttttataat aagcccttct
    10681 gttttatttc gtacatactt aagtgcatta caatcttttt ttatagcttc aagacacaag
    10741 tattctgttt gttcttttac atattcaagt gctttataat tatactttat tgcctctaaa
    10801 cataattctt cggtttgatt ttttacatac tctaatgcct tgtaattctg ccttattgct
    10861 aaaatacaca taccatcagt ttgattttct atatttttta attcacgata atcgtatttt
    10921 accctttcca tattttcaat atattcatta gtgatatact ttaaagaatc tcctaatttt
    10981 gtatcataaa taccaatatg aatatcttcc caattactat taaaataata ccctttatca
    11041 tctaaaaata atttttttat ttttttagat aaatgcatga atatacaccc ctatcattat
    11101 taaattagtt attattaaaa gtttgattat tagtctttat agttttttaa taataacttt
    11161 attttttgtt tattataaat tcgctctgta tattgtagat acctttttca tattttaata
    11221 gttaatcgtt ttaattcttc ctttatttta aaatttaaat ttgtctaaat atttcttatt
    11281 ttatatacat tttttgataa attaataaag ccataatata tttaatatat caatcacaat
    11341 ataggactga agttataaat aaattatggc tattcaatac atttaatttt atattttctt
    11401 gtctaattat ttctttatta tatttcccat ttttttataa agtggcaatg ttactaaaga
    11461 tattataaca gccttcagca cattaaatgg agctatcatc caaattacaa aagtgaataa
    11521 atcatgaact tgaggattta tagctgaacc taaacctatt atagcatcta gtcccataag
    11581 ttgaccataa agtggcaaca ttacaaaata atttactaat gaaccaatta tagtcataga
    11641 tattgttcct aatacaaaac caatcagtac accttttaaa tctccacttc tcttatatga
    11701 ataacaaagt atcattacat aagaaccacc aattaatata tttgcaaatt ctcctattcc
    11761 tccagttgtt gttgctgtta ttgcttgtaa cacatttttt actacaacta ttccaaatcc
    11821 aactaatggt cctagagaca ttcctccgaa tattgctgga atatctgata agtcaatttt
    11881 taagaaatct ggaaatatac ctggtattgg tgcttgaata aacattaata aataagatat
    11941 tgcagataaa attgacatta ctactaaagt tcttgttgaa atcatattgc ctctttttgc
    12001 tgtgttttgc ataattattt tccccctaat caatatgttt atatttttta ggaaaaacaa
    12061 aaaactcgaa gaccctatct tcgagttttt atggatatag tttaaacaaa ctaaaataaa
    12121 cttatttatc ttccataaat atcttctctc atccagactt tactgtcggc tttggaatct
    12181 caccaaatca accacctaag tagttcgcgg gctataccgc cggtagggaa tttcaccctg
    12241 ccccgaagat cttctttgat tttttaattt tccttacact cttatattag ttaatttttt
    12301 aataattgtc aagttatttt ttctcataca ctactttgcc atttattata gtgtaaagta
    12361 cattactttg aatctctagt ggagaattat cccaaattac taggtctgca tctttaccaa
    12421 cttctataga gcctactcta tcttctattc ctaaagtctt agctggattt atcgtaattg
    12481 attctattgc tttttcttct ttcataccat gttttactgc aatacctgca catattggaa
    12541 gatattgaac tggtattact ggatggtctg tcattataca tacatcaaga cctgcatttg
    12601 aaagtattcc tgctgtatta aatgtcaaat ttctaagctc aaatttagac ctttctgaca
    12661 aagatggccc tactattaca ggaaaccctt cttccactaa ttcatctaca ataagatgac
    12721 cttctgtaca atggtctaat gtaagcttta aattaaattc ttttgcaatt cttattgctg
    12781 taaacatatc atctgctcta tgagcatgtg ccttaaaagg aatttctctt cttaaaactg
    12841 gtattaaact ttccattttt atatcatatt caggtttttc acagtcttca tcatcatgac
    12901 tctcatacat atcaatatcc tctaaatatt cttctgcttt ttttaggttt tctctaagta
    12961 aagcagctat agccattcta gtttgaggag atttatcatc ctgaccataa cagctctttg
    13021 gattttctcc aaaagcaatt ttagatgcaa ctggattttt tattaccatt ttatcaattc
    13081 tttttccaca tgtctttata gctatacatt gaccacccat aacatttgca cttccagggg
    13141 ttgtacaaac agaagttatt ccaccttcaa aagcttcttt aaaagttcta tccattggat
    13201 taattccatc tataggattt aattgtggtg ttattgggtc agtctcttca tttccatctg
    13261 ctccttcaaa ccctatacca tcttcccata aacctaagtg agtatgagca tctataaatc
    13321 caggaaacac aagtttatta ctagcatcta ttacctctac atctaaagga gctatcaaat
    13381 cttctccaat ttctattatt tttccttcat ctataagtat atctccatga attatcccat
    13441 ttgttatggt atttatttta ccatttttta taaaaatcat tcattttcct cctgattttc
    13501 aagactatcg ttcttatttt aatatatttt cttactatat acttttacaa atttttaaca
    13561 tttatacact cgagtaatct catttacaaa tatagaattt aagtactata accatacata
    13621 aacttatcta ttttttcatt gataaggcaa ctcttttttt gtttaagtca attccaatga
    13681 cttttacatc tactatatca cctactgtaa caatactcat agggtctttc acaaaactat
    13741 tactcatttc tgatttatga actaatccgt cattttttat tccaatatct acaaatgcac
    13801 caaagtctac aacatttctt attgtacctt ttaaagtcat accctcttgt atatcttcaa
    13861 tttttaagac atcagttcta agtataggtt ttataccttc ttctcttggg tctcttcctg
    13921 gttttttgat ttcagctatg atatctttta acgtaacttg tcctacttcc agtttttcac
    13981 ttaattccct caagccaatt tcttttattt tttcatctat ttcacctata ttcttattct
    14041 ttacatcatc taaagaatat ccaattatct caatcatttt tttacatata tcataacttt
    14101 caggatgcac acctgtatta tctagtggat ttttaccctc taagattctc ataaatccag
    14161 cacattgagt aaatgcttga ggtcctagtc tttttacttt tttcaattgt gctcttgatg
    14221 taaaatctcc attttcttct ctatatgcta ttatattttt tgctatagct ttacttattc
    14281 ctgcaacatg ttctagtagg gagtatgatg cggtatttaa atctactcca acactattta
    14341 cagaatcttc aacaactcca tctagcactt cttctaatct ctttttgttt aaatcatgct
    14401 gatattgacc aacaccaata cttttagggt ctatttttac aagctctgct aatgggtctt
    14461 gtaatcttct agctatagat atagctcctc ttattgatac atttatatct ggatgttcct
    14521 catttgcaag ttcagatgct gaataaacag atgctcctgc ttcactaact atcacatact
    14581 gcacttcgct atctatttct tttatcattt ctgatacaaa agtttctgat tctctagatg
    14641 cagtaccatt tccaattgaa attatatcta tatcgtattt ttcaatcaat ccttttaaaa
    14701 ctttctttgc tccctctaca tcattttgag ggtctgttgg atatactgtt gtataatcta
    14761 gtagtttacc atttttatct actacagcta ttttacatcc agttctaaat gcagggtcaa
    14821 atcccataac tactttgtct ttaactggtg gttgaagcaa taaactttta acattctttc
    14881 caaatacact tatagctctt tcttgagcta tttctgttaa atgatttctt atttctcttt
    14941 ctattgatgg gaaaattaat cttttataag aatcttctat tgaacttacg atttcttctt
    15001 tatttttgaa atttttatca tttacatatt cgtttattat gtaatttaaa actttatcat
    15061 tatttatttc taacttaacc tttaggaagc tttctttttc tcctctgttt attgccaaaa
    15121 ctctatgtgg agccatactt tttactgctt cgttataatc ataatacata tcataaaccg
    15181 atttctcatc agtagcactt ttgctaacta tcataccttc tcttaaagct aattctctta
    15241 tatactttct aatcttagca tcatctgaga ctagttctgc aattatatct ctagcacctt
    15301 ttagagcatc ttcaatagaa agaacttctt tctcttcatc aagataattc tttgcctcta
    15361 tttctatatt atctaaatta ttctctagta ttgacaatgc taaattttct aatccttttt
    15421 cttttgctat tgttgctcta gttcttttct tttgtttata tggagcatat atatcttcta
    15481 cttcttgtaa tgtttttgct ttttctatat tttgtgttat ttcatctgtt aatttccctt
    15541 gttcatctat tagtctaact acatcatctt ttctactttg aagatttctc aaatacatta
    15601 atttttcata aaattctctt agtgtaacat cactcatttc gccagtcatt tcttttctat
    15661 atctagctat aaatggtatt gtatttcctt catcaataag ttttaatgta ttatttattt
    15721 gttcatctct aagattaaac tcttttttta agatttgatt tatatccaat ttattcactc
    15781 ctaatgttag tttttcataa gtataacaaa acagaacgaa ggctgagggt tatcgtcctc
    15841 agcctaagtg ctgtaagtgc atattaagca ctaaccttct tatacatata tcttaattac
    15901 agtgcatatt tattaaacta ttttattcat ttttaagtat tcattaataa acaagtctat
    15961 atttccattc attacactat ctacatttcc aaactcagca tttgttctat ggtccttaac
    16021 caatttatat ggttgaaaaa catatgacct aatttgacta ccccaagtta tttgtgagta
    16081 tttgccttgt atatcttcta ttttttcctt ttgctctaac tcttttaatt ctatcaattt
    16141 cgccattaaa agtctcatag ctctgtcctt atttagatgc tgagaccttt cattttggca
    16201 ctgaactact acaccagtag ggatatgtgt tattctaact gctgaatcag tagtatttac
    16261 atgttgccct ccagctccag aagctctata tgtgtctatc tttaaatctg acggattaat
    16321 ttctacttct atattttcat ctaactctgg tgttacatct atagatgcaa atgaagtatg
    16381 tcttttccct gatgggtcaa atggagatat tctcactaat ctatgaactc ctttttcact
    16441 ctttaaatac ccataagcat ttgttccttc tacaagaatt gtagctgtct ttatccctgc
    16501 ttcagggtct gatattatat ctaaaagctt aactttataa cccttacttt ctgaccatct
    16561 tatatacatt ctcaaaagca tttgagccca atcttgagca tctaagccac cagtaccagc
    16621 atttatagat aatatagcat tatttttatc atactctcca tttagaagag tctttatttt
    16681 catttcagat aatttttctt ccattgattc tatggacttt tctatatctc tttctacaga
    16741 atcatcatct tcttctaaac ctatttcaat taatacttct atatcttcta ataagctttt
    16801 taagctttca tattcttcta atgtctcttt taaagattta ttttcttgaa gtactctttg
    16861 agcgacttca ttatcattcc aaaagtcttg ttgattcatc ttttcttcat taatttgtat
    16921 atttttcatc aatgaagcta tgtcaaagag aaaccctcaa ttcttctaaa tttgaagcca
    16981 tctgctccat actatgtcta tactcttgta actttaacat ttaatcctcc gagattatct
    17041 tccacaacag tttttatatt ttttaccact tccacaaggg cataagtcat ttcttccaac
    17101 cttttcatct tttttaacag ttttacttgt tggaagtgtt ccatctgatg gagaagatag
    17161 attttcaaca tctacaactg cttttctttc cactggagtt tctattgtta tattaaataa
    17221 atatcttaca gtgtcttctt tgatatgttt attcatttca tcaaacatat caaaaccttc
    17281 cattttataa gcaataactg ggtcttgctg acctacagca cgaagaccaa taccttggcg
    17341 taattggtcc atagcatcta tatggtctat ccaatggtta tcaacagctt gaagtaagat
    17401 tactctctca acttctctca ttctctcata gccaacttgt tcttcttttg aagtatatat
    17461 cttcatagct atttcataaa ctttttctgt tatttcaact ggattaagtt tttctatttc
    17521 tggtatttct atgctacctt ttggcaagaa cagattatac atatgttctt tgaatccttc
    17581 ttcatcaaat cctttatctt gtgtataaag agtaactgct tcttctatta tagaatgagt
    17641 cattgattgt atttgttctt gtaaatcttc accttctaat acacgttttc tctctgcata
    17701 tattatttct ctttgtttat tcataacatc atcatattga agtacatgct ttcttattcc
    17761 aaagttttta ccttcgactt tcttttgtgc tccttcaata gacttagata atattctatg
    17821 ttctatcggc atgtcttctt caagacctat tttatctaca ataccagata ttctatcact
    17881 accaaacaat ctcataaggt catcatcaag acctatatag aatcttgaac tccctgggtc
    17941 accttgacga ccagcacgac ctctcaactg gttatctatt cttcgagatt cgtgtctttc
    18001 tgtacctatt atagcaagtc cacctgcttc tacaacttgt ttttgttctt cttgtgtttg
    18061 ttgcttaaac ttttcaaata gtttttcata ctcttctcta gcttcaaata gaatttcatt
    18121 accttttaca ggtatacctt ctattggagt atctacccta tttactattt cctctttaaa
    18181 tccatttctt ctcatttctc tcttagttaa gaaatctggg tttccaccta aaactatatc
    18241 tgtaccacgg ccagccatat ttgtagctat tgtaacagcg cctaatctac cagcctgtgc
    18301 tattatctct gcctctttat catgatgctt agcatttaat acttcatgtt taatgccttt
    18361 tttctttaat atttgtgata ataattctga tttttctatc gatactgtac ctacaagtat
    18421 tggctgatta actttatgtc tttcaattat ttcttgtgct acagcattaa atttacctat
    18481 ttcacttttg tatacagaat ctggtaaatc ttctcttacc ataagcttat tggtaggtac
    18541 ttggaaaacg tccattttat aaattgcttt aaactcttct tcttctgttt ttgctgtacc
    18601 agtcatacca gaaagttttt tatacattct aaagtagttt tggaatgtta cagtagctag
    18661 tgttttagat tctctttgta ttttaagacc ttctttagcc tctattgctt gatgtaaacc
    18721 ttctgaatat cttctaccaa acataagtct acctgtaaac tcatctacta ttactatttc
    18781 tccatcttta gcaacataat caacatcttt cttcattatt acatgtgctc ttaaagcttg
    18841 atttatatga tggtataatt ctgtatgaga tatatctgtt atattatcaa cattaaaata
    18901 cacttctgct ttttgtatac ccgatgctgt tagagataca gccttatctt tttcttctaa
    18961 ttcataatca tctggtttta aggttaaaac aaatgtgttt gcatctgaat ataagtgagt
    19021 tgatttgtct ccaggtccag atatgataag tggtgttcta gcctcatcta ttagtataga
    19081 gtcaacctca tccactatag cataatttaa tcctctttga actctttgct ctttgtgtat
    19141 taccatattg tcctttagat agtcaaatcc atattcatta tttgtaccat aagttatatc
    19201 acaatcatat tgttcttttc taacttttgg attttgaccg tgtataataa cgcctacact
    19261 cattcctaaa aactcataga tttttgccat ttggtctcta tcacgttttg caaggtaatc
    19321 atttactgtt acaacatgta caccttttcc agtaagcgca ttcaaataaa caggagcagt
    19381 tgcaaccaaa gttttacctt cacctgtttt catttctgct attcttcctt gatgtagtac
    19441 tatacctcca atcatttgaa ctctgtagtg tctaagtcct aacactctct tagaaacttc
    19501 tctaactact gcaaaagctt ctggaagtat atcatctata ctttcaccat ttgctaatct
    19561 ttctttaaat atatttgtca tatttttaag ttccgagtct gccatagatt caaatttagg
    19621 ttctaatgaa tctattatat caacagtttt attgaacttt tttaattctt ttttatctgc
    19681 catgttgaat aaattatcca taaagctcat attttacatc tctcctcgtt ctttattgta
    19741 ctattattca ttttaccata aaaactatac ttttaatatt aatttaaaaa gctatcgata
    19801 atttatttat cgatagctaa tatgattaaa tctaaattgc tttattcatg ttctattata
    19861 ccatatccac cattattacg cttataaact actgctattt catctgaatc tatgttcttg
    19921 aacatataaa aatcatgtcc tattaattcc atctgtaata cagcttcttc ttcactcatt
    19981 ggcttaacac taaatttctt acgtctttga attactaatt cttgattttc atccccataa
    20041 tcctcttgtt catcagctag atttatctct gaatcttgga atcttatact cttgttgtct
    20101 ttatgtttat cttgtaatct ctttttatat ttttttaatt gtttacttaa tttatcatat
    20161 acaacatcta tagctgtgta taaattttcc tgcatatctt ctgctctaat tataggacca
    20221 tttacagtag ctattgttac ttctattttt tgtcttgctt ttctcgcact tactgtaact
    20281 tttacatcag tgtctggatg aatgtaatta tctaacttac ttagtttagt ttcgatttta
    20341 ttttttattg catcagttaa ttcgatttgt tttccagata taattatgtt cataaataca
    20401 tctcccttca attatgtcag tataagaaaa tttatacttt aataatatat tctacacgaa
    20461 ctctcataat cctcttttta ttatttactt ttatgatttt ttctattcaa ttgatagaat
    20521 tatttatcat ttttagttac attgatactt atgtggatta atattattac aaacacctgt
    20581 ggataatgtg gataaaactg ttaataactc ttgtttttgt ttatttaaag ctatttttac
    20641 tcctatttct atccacaaaa atctccatta attgtttatt aatatgtgaa ttcatgtcat
    20701 aagatggtaa ttttaggaaa ttatgtgtat tatatgccaa tataccttta taatttttta
    20761 ataactataa accctctaaa acctccataa tctatctttt ctttcattat ttatatttaa
    20821 aactacttaa aagttataaa gcatactcaa caataatttc atatttaata cctattaaca
    20881 ttcatatacc caaattataa ttaacttact ctctttattt aaactaagta ttttatattc
    20941 atatactatc ttgaaattat atttacgagt tgtttaagct tttgtctatc tatatcttca
    21001 aaaggactgt tatttattct aatcgctgta ccaaaatgaa aattacttgc tttagttaat
    21061 tcctttatct tttctatatt attaaaatta agacctcctc ctaaaagaat ttttatgtga
    21121 ttagaactaa gcatcatatt ctttatcatt tgtatgttat caactatact tcctttccct
    21181 cctgatgtta atatattcgt aattttatca taatccttca aaattttaac actatctatt
    21241 gtatttgatt catctatggc tctatgaaat gtaacatcaa gattatcaca acatttcaat
    21301 aaaacattta gatttttctc atctatatta ttgtttttat ccaatacacc aaatacaact
    21361 ccattagcac caatctcttt aaccacacct atatcttttt gcattatact tatatcttct
    21421 ttactgtata caaaactttt cgcatgatgt cttatcatta cgttcacagg tatctttaca
    21481 ctgttaacaa cactttctat aagaccaaaa cttggtgtca atccaccttc agttaaagca
    21541 ctaactaact ctattctatc tgcaccacaa tcttctataa ttttagcgtc ttctactgac
    21601 attcctatta tttctaacat aactactcct ttgctaattc atatattgca tttgcaaata
    21661 tctttgcatt taatattata tcttctatat ccatgtattc atcaggatta tgagctattc
    21721 ctttttgtcc tggaaatgaa ggtccaaacg gaactatatt tggcattact tttgcatacg
    21781 tacctccatt agttgtcacg ggagttccat ctaatgatgt aaccctttca taagctagct
    21841 gaagcttttt aacaagatta ctctctttat caaaatatac aggattaaaa ttagaaatta
    21901 cctctatatc gatactattt ggaaaactag cttttatttc atctaataca tctttcaatt
    21961 tataactaaa atcgtatttt acctttatat ctaatatagt attactattt tctatattca
    22021 gctctttata tgtaacaagc acattttcag acatattttc tataaaacct aggtatacat
    22081 cttcttcata attgatttta ctttttattc taatctttgc catgcctttt tcaccatata
    22141 caacaggata tttacaatct ggtgtaaatc ccataatagg tgttttttct ttttctaaat
    22201 aatatggtat atcttcaaat ccactttctt catttgtacc aaatattatt ctaactctct
    22261 tatctaattt taagtttaat tctttaatag catataatcc atataaggca gacattatag
    22321 gtcctttatt gtctagtact cctcgtccat aaattcttcc attagtttct tctcccttat
    22381 aaggctgatg cttccaacca tctccttcat gtacaacatc tacatgtcca attacacaaa
    22441 cataatcatc actatctcca tgctctgcat aaccaatata attatccaag tttctaacct
    22501 taaatcccaa tttttttgat atttctaatg ctttattaag tgcctcccct actttttcgc
    22561 caaaagggaa gccattttct gactcccctt tgacactagg tatttttaca atatctataa
    22621 tatcacttaa taaatcttct tttaactcat ctattttatt acttatgata ttcataaaaa
    22681 tctccttatt ataaaactct attttaatta tgtaacacta ctttactcac ttacttctat
    22741 cggtgctttt attctagcct catatgcttc catccactca tttgatataa ccttatgtat
    22801 agtctttcca tctactctat ttgaaatata tacagcagga gattcttctt tagttactac
    22861 tgaaaaagtg aaatcaacta tatttgtagc tactccccat tcacctttat tattcatggc
    22921 aactaatgat aaatcaccag cttttcctct tcttttgata agctgtttat caagttcatt
    22981 tactgcaata tcacatgctt cttgtggatg ctttccttct ttcataagcc taactatctc
    23041 ataagatata catcccttca ttaaatcttc tcccaaacca gtagctgtag ctcctcctat
    23101 actactatca acatagaatc ctgaaccaga tagtgcagaa tctcctattc tacctctttt
    23161 cttcataaac aatccacttg ttgatgtagc tgcacacatt tttccagttt tgtctaaaga
    23221 tatcatacca acagtatcat gaccagcata tgggcttaat cctttgtcta aagtctcttt
    23281 ttttctcttt ttataatgta acttcgctct atcagtcaac atattttttc tttcaaatcc
    23341 atttttatga gcataatctt cagcacctat tccaactaaa aaattattta ccttctcata
    23401 acttaacttc ctagctatgc tgacaggatt agaataatct cttataccag caactgcgcc
    23461 aatatctaat gtgttaccat ccataaatgc agcgtcaagt tctacctcac aattttcatt
    23521 tggaagtcca ccatatccaa ccgatttata gaatggatag tcttcaactc ttcttacagc
    23581 aagctctaca gcatcacctg cttccatgcc tttttctaaa tgttctgatg cctcagttat
    23641 tccttctagg gccattctcc atgtcgctat cattccccac atgttaaaat tcccctcctt
    23701 aaattatata gattttacta atgtatgctt attataaata gtataatatt taaacttgct
    23761 aataattaat actcttatta ataacaaaat tcttctttca tcaaagttat atcttcttta
    23821 ttcgctattg cttttgccaa tttacatatg ttatttaatg aatcattaag accaattcca
    23881 ccttttttgg gagtcttaac tatacatatg tcatttttat attcgtttat cacatctata
    23941 ttttcttctg acatagctgc aaaagctggt atatctgttt tatcatttat atttttagac
    24001 agtattgctg acatcttaga aaagttttcg taattaaagc ttggtccaca gattactaca
    24061 tcagggttta atttttttac catagctatc atcttactcg ttacttcttt ttcattattt
    24121 acaaaaaatt catccccaca aaataaggta gctattacct tagattcatt taaaaattgc
    24181 tccatcatta cacctggtcc taatgggctt gattttccag caggaggtat attacttttc
    24241 tcttttcctc cagctccagc ttgtatttga tccaatatca taactatttt cttcataatc
    24301 ttcctcctat aatactaaat catcaaaaga tatatcatca tcttcctctt caacagtttc
    24361 attttcttct ctcaagtatt gcttatctag catttttata aatggtatat acataaatat
    24421 tcctagtata agaagcacta attgtaagac tgcaccttgc catcctgttg ttaaaaaacc
    24481 agagaatata attggagttg tccacggtaa ctgaactcca tttgtaagag gcactaatcc
    24541 tgctgacata cagaagtaag ctattattat atttattgtt ggaactattg caaatggtat
    24601 caatagtatt gggttaagca ttattggtag accaaatatc aatggctcat ttataccaaa
    24661 tattcctggt aaaattgata gttttcccat tgactttata cgtttacttt tacatattaa
    24721 taacatagct atgactaatg atagtgtact acctgctcca ccaaatgtcg caaacatatc
    24781 ttggaattga cctgttatta tgtttggtat ttttgaacca gcctgaaaag cagctaagtt
    24841 ttcagctgat agaattttta atattggatt atacacagcc ccaacaacac tacttccatt
    24901 tattccaaag aaccagaata aatgtaagaa tatataagcg attgccatag cacctaaact
    24961 atttcctaat tttaataatg gcatttgtaa aaacttaaat ataaattcat gtatacttcc
    25021 atatggtgta gcttcaaaag ctatcttaac taaaaagaaa actaccataa ctattgcact
    25081 tggtattaat gctgcaaatg attttgatac ggttggagga actccatctg gcattttcaa
    25141 tgtccatcct ttttttacaa ctgcagcaaa taattttaca gaggttattg ctgttatcat
    25201 acctacaaac attcccttag agcccatcca acctagtggt atacctgtta ccatgtagac
    25261 tgattctgtt ccttctggtg tatatggtat tacaaacggt gttactataa aaaaggcaac
    25321 tattgcaact gcagctgatg ctattggatc tacatctatt tgctttgcat aagaatatcc
    25381 aattcctaaa actgcaagta aagtcattac ttcaaatgtt gctacagttg gttgttgcaa
    25441 ttttacagcc caatctggac caaaaatttg acctacaaat tcattccatc cttttatagg
    25501 aaaatttgct actaataaaa ataatgaacc tattattaat aatggtgatg atattaaaaa
    25561 accatctcta attgcaatta ataatttatt tttacctatt ttttcagcca atggcataag
    25621 tattctttcc aatctattca tctatccacc ctcatttcct attatttttt actataattc
    25681 tacgcttgtt tagattttat tagctttata gcaagtttta atactttttc cccattcata
    25741 gttccataat caacttcatt gattatatct ataagttttc cttgagcttc aaactttggt
    25801 tttaactcct ttagcatgta tttaacttgt ggtcctaata atatacaatc tggatttttt
    25861 tcttcaacta ttttgtcaat ttctgctatc ggaaatgcct ctacttctat tggtaaattg
    25921 tgttcatctg ctaccttctg cattttatct gcaagtaaac ttgttgacat accaaagcta
    25981 caaaataaat atacttttct tttcattctt ttaaatcccc cttaattttt ttattttaat
    26041 ttttgaattt cttttcttaa cataatcatt tcttttataa ggtttctctc actcatacaa
    26101 gtcattaaat ggtcttgaga gtgaatcaat aaaatagata tatctgattt atctccccca
    26161 gcttcctttt gtataagttc agtttgtact ttatgtgctt ttaaaatttc ttcatttgca
    26221 ttttcaatta attcatctgc ttttttaaaa tcgttttcct ttgcatagtc aagtgcttca
    26281 taaagcatag attttgcatt tccagcatga attataatct ttaaaactat ttcttccatt
    26341 ttttcctcct aaattgcttc tctactttta gattatttat accttacaaa catataatat
    26401 cttataaaaa caaaaaaaca tgcacaactt tttccatcac atggaaaatt ttgtgcttat
    26461 ccatagttca ttatgaattt tatataagaa tatcttttac cttttcaatg tcacatttac
    26521 ttagaatatc tagcatattt tcatcctctg ttaaattaga tatttcttta aataattctc
    26581 taaatacttt tctatcatca tcttttttat atgctaataa aagtataatc tttatatcct
    26641 ttccatttct aatttttatt ggtgttttta accttgcaaa acataaaaca gattgtttta
    26701 tatatttcat gtctccatga gggaataata ttccttttcc aacattggtt ggatatgact
    26761 tttctcttgt tataatagat tgtaaatact ctttttctgc ataatgtttt tcaattaata
    26821 aattagttaa atatgttata gcttcataaa tatcatcatg ttctaactct cttattaaat
    26881 aatcagacat cattaatctg ttcataagtt tatatgaggt gtctttttct tttattttgt
    26941 ttgtaaactc tttaagttta ttctcatcct catcagtaaa taatggtgtt gttataaaaa
    27001 ttggtttttg gatattttca tactcttgaa atgcaattat aaaatctata gtcttattaa
    27061 agttatctct ttttaaatct tcctctctta atgtttcaat aatttttatg ttatcaaatc
    27121 tcttttctat ctttactttt aatagcatac ttactccaaa actaaatgga catacaatac
    27181 ttgccttttt atttgattcc cttttgctgt attttctttc tagagaggtt tgaaagtgaa
    27241 gtgtaagata accaatttca tcttccgtta tttgttttcc ttttaattca atttctattg
    27301 aacttgatat tactgtaaat aaaaaacaaa atttagtttt tatgttatct aaaagtggat
    27361 tttctaagta aacattgctt ttcatttgat gcattgtaac attcaaatga catattaatt
    27421 gttcataaag taatatatca ttattaaaat ttattcctga ttcttctgat actaaattta
    27481 taattttttt tgtataactt gctaattctt catttagata taaagatttc ttaatattat
    27541 tactatttat ttgtttgttc atacccaata ttaaagattc tataaacaat ttttcttcat
    27601 aatttaatac tatggataaa ttagatttca attcatcttc tatatccaat ataagcattt
    27661 ttagcttaag ctctttctta gtatttgata cttttaaaga atttcccagc cttattcgta
    27721 ttatatttat aagcaaaaat agcatcaatt gcttgaatga tatatctgta agtcttatat
    27781 ccatatcctc ctctatagta agcattatgt tttttacaat tattatatct actatatgga
    27841 agtactctat ttcatctatg tttaatctgt atttatctat gtatttaaat aagaatgata
    27901 ctaacattat tcttatgtct ttctcatccc cttcaatata tataccactg ccttttttag
    27961 tcgagacatt taaattataa ttatttaatg ctactgataa tttacttatt tcttctctga
    28021 caatattttt atttatgtac aacttattag ataaatcatt taaagtaact tttttattat
    28081 tacaaagtag taaatttaat atctcaactt gcttatgaag ctcactattt actcttttaa
    28141 cttcacctcg caacttctga tttaccagta ttttgtctgt tccatctcca ctaaatttaa
    28201 ttccaatatt aggttttctt ttgatataag cattacaaaa agtagataac cattcatcaa
    28261 tatatttgca atcattcctt attgtccttt ctgaacactc aaaatcattg gcaatcttat
    28321 taacatttat aaactcttct tttaatagca atgttgttaa taaattttct tgtcttttat
    28381 tcaaacatat cacctcttta tgactcaacc taaatttatt atatataaaa aagagctata
    28441 cataaaggta tgctagctct cttttaatat gatatttagc tgacctggtt tttgacccca
    28501 cactcgccta ctggagggtt cgcttgggtt cgccgcacta ctactctctc tcgattttat
    28561 tatcggtagg tcttacttct aagccgcacc atgatcatta acccaatttt tagtgttaac
    28621 ttacgtggat ccctttgcct gcgactggct tggactttca accacagacc taaatatcac
    28681 ttattcattt taatatttta aactaaaatt gtcaatatat aagattattc cataatataa
    28741 ttatcatttg cttttgtaag tagagtcaat acaaaaacct tattagcacc tgataacttt
    28801 aaaagtttag agatttcgtt tgttgttaga ccagtagtaa atatgtcatc tattaaaagt
    28861 atatttttat tatttattaa tttaacattt tctttaacaa caaatacatt ttttaattct
    28921 tcttttcgtt ctttcttatt caatttatat agcattcttg tatatttttt tctacttatg
    28981 caatctaata atggaatatt tactatttta cttaaattaa atgctatttt ttgtgcctga
    29041 ttaaaacctc ttttatttag ccttttttta tgaagtggaa caaataatat ataatcaaat
    29101 tttatgtttt ctaaatataa tttttctttc attatttgag ctatatactt agccataaag
    29161 gttttttggt tatacttcag ccctaacacc atcttttttg tcacatcatt atattcaata
    29221 caagatattg atttatcaaa ataaaagtct ttattaaaac aataactaca ctcttcaata
    29281 aattcttttt caattgaatg acgtattatt ggttttccac acttcatgca accatcttgt
    29341 ataaaattca tttctttaaa acaactttta catattgaat aagtatttgt tttctttatg
    29401 gatttatcac aaattatgca acttatattc tcaggatata taaaatccaa acatttatta
    29461 atcatatttt taaaattctt aataaaatta tcattgcata ttttcatatt tattctatca
    29521 acaacccttc ttgtttaaat ttatttagct tatatcctaa atttgaatac ctttgattaa
    29581 ctctattatt ttttatcata tattcaagat atttcacatc tccaactaat acaactaatt
    29641 tttttgctct tgttactgct gtatataata aattacggct aagtaacata ggaggagccc
    29701 acgctattgg caatactaca actggaaatt cacttccctg gcttttatgt attgttgtac
    29761 aaaaactatg gtcaatttca tctaattcat catataggta tgatacaatt ttagtctggt
    29821 caaataatat ataaatagtt ttcttatctt tatctatgtg ataaacatat cctatatctc
    29881 cattatagat accttctcca ctttcttttt ggtcttctgt ttcccatttt tttgtataat
    29941 tattttttac ttgcattacc ttatcgccaa ctctaaataa tcttttagaa aaactttctt
    30001 caactttaaa tttttctttt ttgttaagat acttctgtaa ctctatattg agattagtaa
    30061 ctcccaattc accttttctc attgaagata aaacttgtat atcttttaat ttatcaactt
    30121 tataaaattt aggtaatctt tcattaacca atcctattat ttcatttaaa atttcttcat
    30181 tagttgattt tcttataaaa aagaaatctt taccttttgt atttagataa agtggttctc
    30241 cattgtttat cttatgggca tttacaatta tcatactttc ttgcgcttgt ctaaatattt
    30301 catttaattt aactacattt ataatattag aattaatcat atccttaagt acatttccag
    30361 cacctacaga aggtaattgg tcactatctc caactaaaat tactcttgtt cctaacttta
    30421 tagctctaag taaattgtac attaagataa tgtctaccat tgatacctca tcaacaatta
    30481 ttacatctga atttattggg tcttcttcat ccttaaaaaa agttaaatca tcatctgttg
    30541 aaaatcccat ttctagcaac ctatgtatag tttttgcttc tttattagaa gtttcactca
    30601 ttctctttgc tgctcttccc gttggagctg ccaaagtcac acttttacca ttattttcaa
    30661 atatatctat gatagcatta atggtagttg ttttaccagt tcctggtcca ccagttataa
    30721 ttaatactcc actattaaca gattctttga ctgctaaaat ttgtttttct gctaacttaa
    30781 ttttcttatc ttcttctaaa acattaatct cgttatctat atctattttt aagtcttcaa
    30841 attcatactg tgataatttt actatttgac tacatactcc attttcagct aaatagtatg
    30901 gcataaggta tataagattt tcattgttta ctttctctat atgtattttt tgattataaa
    30961 ctagcatcat tacacactct tttattattt ctccatttag ctccaataat ttttccgaat
    31021 cttgtattag aacatgctct ggtaaatatg tatgcccatt acttaaagat ttatttaaag
    31081 tgtataagat tccctgacaa attctatctt tagagttttt atctatacca attttacttg
    31141 ctatactatc agctacttta aatccaattc ctcttatatc ttcagcaagt tggtatgggt
    31201 ttttatttat aacttctatt gcactgcttt tatatttttt atatattttt aggcagtaat
    31261 ttggtgttat tccaaaagga gatagctgta ttattatatt tctcaattct ctgtcttctt
    31321 cataactttt tactatttgt tttacttttt tactccctat accttctact tcttgtaatt
    31381 tttctggtga attttgtatc acctctaacg tatctacacc aaacttatca attattcttt
    31441 ttgccatttt ttcaccaata ccatgtatca ttccagaaga taagtaaaca taaattcctt
    31501 ctaaggatga tggcgttact ggcataaaac tattcacttc aaattgagtc ccataaatct
    31561 tatgatttac ccactttcct tcaacttcta tgctttctcc aattgccaaa gtaggcatac
    31621 atcctacaac cacaatttca tcattttcat ttaccaggtg agctattgta taaccattat
    31681 cttcgttttt aaagactatt tcacttatca ttccttctaa tttttccatt tttatctcct
    31741 aaaaatacta ataatcgctc catttaaatg ttagtataat tttatatatt aaaactatga
    31801 taatcgtaga aaagttttat atttttatac ttaacgtaat ttccaactat tatacatttt
    31861 atcagaaaca aaaaaaatct gcatccataa aatatggtac catgaaatac aggtaccata
    31921 tttaaatagc tattttattt taattactct atatttagag tataaattaa attccataag
    31981 tatattacag tactaactta tttcagattt attctccttg tacccctcag ttttagtaat
    32041 tcttctataa agcatacata gtaaaattaa aattcccatg taacctgtat atggatatag
    32101 tgtacctact aacttatcaa atggcagtaa acctcctata caagctaata tacttaccac
    32161 tattgttata attttaaatt tagggtggtc atcttctaca aatcggttag ttactgacca
    32221 taacaatggt acagctgtag tgtatattcc tagtaaaagt actactgaga ataaaatacc
    32281 tattattgga gatatcttat cagctaagta aagtgctggt atctcttttg tgtatatatt
    32341 tcctatgttt gaaagtaatg ccaaattcat cattatagct gctgccataa gtgctactcc
    32401 acctactata ccaccccata cagcatcttt cttatttact gcagaagctc ccattcctgt
    32461 caagaatgtt attactatta ttacattgta acaagtataa agtactcccg agaaatatcc
    32521 atttgatgta gcagttttaa tatttaaact atcaaccata tttccagcat ttgataaagc
    32581 atcaatattg ctaaataaac taattgctcc tactaaaagt gtaaatatta ttattatagg
    32641 tcctatatta cctagtattt tagaaagcct tgtcaaacct aatgaaacag ttattaggga
    32701 aacgattgcc ataccaatcc tacctacata tggatttaat ccatagtact ctgatagtgt
    32761 agctcctgct cctgcaatca ttattactaa tgttccaaat aaaaacaatg gaccaaacca
    32821 ttcaaaaaat gttccaagat attttccaca atatacttga taaattttta ttggctcctt
    32881 aagtttcaat tctcttccct tatctataac ttctgctcca aaccatgaaa atagtaccat
    32941 agagattatt ccacctataa tacccgcata tccataaaac gtaaaaaatt gcagtatttc
    33001 ttgaccagtt gcaaatccag aacctattac agtagcaaca taagctccag caaaacttat
    33061 aaccgtttta atatttactt tgcctgacat acactactcc cccccaatta aaattatttt
    33121 taaagtgtaa gtttattaag attatattag actcattatt gtgatatttc aatatatttt
    33181 taattattta acattagtta acacattctt aaccatcctt taacatatat cccataatat
    33241 gtataaattt agtagagtat tatataaaat gtattgacca gattaaatac tgtttattca
    33301 ttatatgatg ataaattaac atttattaaa aatatttaac tcataataaa aaaactgtta
    33361 gaaagtaatc gtaaaattac attctaacag ctttttagtt aaaaattatt cacctaaagc
    33421 atcttttctt aactgctcaa ccttgtcagt tctttcccaa ggtaaatcta tatcagttct
    33481 accaaagtga ccataagcag caaccttttt ataaagaggc tttcttaaat ctaagtctct
    33541 tattatcgct cccggtctta aatcaaagtg tttatttact aattcaacaa gtttttcttc
    33601 tgaaaccttg cctgtcccaa aagtatctat aaatatagat aatggtctag ctatacctat
    33661 agcatatgca agttctatct cacatttgtc tgcaagacca gccgcaacaa tattttttgc
    33721 aacatatcta gctgcataag cagcagatct atcaactttt gtagggtctt ttcctgagaa
    33781 agctcctcca ccatgtctag aatatccacc ataagtatct attattattt ttcttcctgt
    33841 aagacctgta tctccttgtg gtcctcctat aacgaatctt cctgttggat ttatataaat
    33901 tttagtctct tcatcaagta attctgctgg tataacttcc ttaataacat gattaattaa
    33961 atcttctctt attttatcat ttgatactgt ttcacaatgt tgagctgata taagaactgt
    34021 atgtactctt acagctttac ttccttcata ttcaacagta acttgagttt ttccatctgg
    34081 tcttaaataa tcaactaaac ctgtttttct aacttctgta agccttcttg ataatttatg
    34141 tgccaaagat atcggaagtg gcattaattc tggagtctca ttacatgcaa atccaaacat
    34201 tataccttgg tctccagctc caactttttc tatttcttct tctatctctt ctccagtctt
    34261 actttctaga ccttcatcaa ctcccatagc tatatctcca gattgttcat caatagaagt
    34321 tataactgcg caagtatcac aatcaaatcc atattttgct cttgtatatc caatttctct
    34381 tactgtttct cttactaatt taggaatgtc tacataagca gatgtactta tttctcctgc
    34441 tactaaaact aaaccagtag taactgtagt ttcacaagct actctagatt gtgggtcttt
    34501 ttctaataat gcatctaata ttgaatctga tatttggtca catatcttat caggatgtcc
    34561 ttctgtaact gattctgacg taaataaatg tcttgccatt tttattcccc cttatttatt
    34621 cttaattttt attataggtt ttaccttatt tttaatttta gaaatttact caaaataaaa
    34681 aaacctctcc tttataagaa gaggttaata atcgtaatta catactaatt aactgacctc
    34741 atctttcaag acttttgtct tgtaggaatt agcacctttt cccatttggg tggttgctgg
    34801 gcttcacagg gcctatccct cagcctctct tgataaggta atcaagattt aagttttgtt
    34861 ttactttact agcttactat ttttttaaaa ttttgtcaat atttttagtc atattttttc
    34921 tattcctttt tttgtaagac aaataaaatt ttctatctat ctgaaattta aagttaggtc
    34981 tttttaatgc tttttattca tatatttttt tgaagggggt gttcctattg tatttagcta
    35041 aagtaaacta cagcgatgat gatgttatac agatgctaag ttcagttcta aaaactataa
    35101 ttaatgaaaa tacgattgtt gtttgcatag gaacagacag ggctataggt gatactttag
    35161 gccctttagt tggaacaata cttaagaaca gtaactttaa ataccccgtt tacggtacac
    35221 tagataaccc tattcacgcc ttaaatatat atgagtcttt agatactata aaaaatacac
    35281 atatacaagg taacttcttg gctatagatg cttgcttggg gtcgcagagt aacataggta
    35341 atatacagat tagagaagga cctatcctgc ccggaaaagg tgttggtaaa aaactacctc
    35401 aaataggaaa ttattctata gtaggaattg tagataaaat tgatgaaaat aataaacttt
    35461 catttaataa tatacgcctt agctttatat tagatttagc tgaaacaata gccttggctt
    35521 tattagtatc cacctaatct aaatataaac taaatgataa aattctttat ataaaaaggt
    35581 agatatttta gagtatctga ttaaatactc caaattatct acctttttac ttttatattt
    35641 caagttttta tttttctact aaagcaaaca ttaattcacc tttacagaca agttcttcac
    35701 ctacatatgc cttagcatct gccttagcta tattttttct aagagttgtc atttcaattt
    35761 ccatagttaa tgtatctcct ggtcttactt ctctcttgat tctaacttta tctatacctg
    35821 caaatacacc tagttttcct ttgttttcct ctacagacat cattgccact ccacatactt
    35881 gtgctagagc ttctactatt agtactcctg gcatcaaagg atattctgga aagtgacctt
    35941 ggaaaaatgg ttcattaact gttacattct ttataccaac agctcttttc ccaacttcta
    36001 gctctgtaat cttgtctact aataaaaatg gatatctatg tggtattaat ttttttattt
    36061 catctatatt taacattcta aactctccta attattttct tttgaaagac ccaccagttt
    36121 caagtaagtc tattgaacct aaagctattc ctgtaccttt tgcaacacaa gataatggtt
    36181 catctgctat tgtaacagtt attcctgtcc tctgttctat tattttatct aatccatata
    36241 ataatgaacc tcctccagtc attattatac cgacatctcc tatatctgct gctaactcag
    36301 gaggcgtttt ttccaaaaca gcatgagtag caactactat ttgctcaaca cattctctaa
    36361 gtgcttccaa catttctgtt gaatttatag ttatagaact aggaagtcct gtaattaaat
    36421 ttcttcctgt tatcttcata tatttttctt cttctctctt aaatgcagaa cctatttcaa
    36481 acttgatttt ctctgctgtt ctttctccta taagaagatt gtgttgtttt ttcatatatt
    36541 taactatagc atcatcaaat ctatctccac caacctttat agattcactt acaacagctc
    36601 ctcctagaga tattacagct atatctacag tacctccacc aatatctata atcatattac
    36661 cattaggctg tgatatgtct acaccagctc ctatggcagc tgctattggt tcttctatga
    36721 tatatacttc tctagctcca gcttgtctcg tagcatcttc tactgctctt ttttccactt
    36781 ctgtgacacc tgttggtaca cataccataa ttcttggcat aaaaaatctt ccaaatccct
    36841 ttttatcaac tatcttatct ataaaagatt ttaacatctt ctcagttaca tcataatctg
    36901 aaattacacc atctcttaaa ggtcttatag cgactatatt acctggtgtt ctccctatca
    36961 tctttttagc ttcttctccc acagctaaaa cagaatctgt tcttttgtct atcgccacaa
    37021 cagatggttc ttctaaaacg attcccttac cactaacata aacaagcaca tttgcagtac
    37081 ctaaatctat gcctatatca gctccagcca ttttttcaac tcctttattt ataatattag
    37141 attatattat aagcctattt taatactcct ataataaagc atatacaaaa atctcgcttt
    37201 aatcaagaga aaataaatct tttgagagta ttattatttt gctatttata attacttaat
    37261 atatttcttt ttataattta attcaataaa aaaagtaggt ttttaaccta cattttttta
    37321 tgttccttct catattttaa tttcgttgct aatccccctc taatatgtct ctctgattta
    37381 tttttgtcca gcacattttt aacttctttg gcaagggatg gatttatttc cttcaatctt
    37441 tctgtaacat ccttatgaat tgtactttta cttactccaa atgtcttggc ggtttgtctt
    37501 actgtagtgt ttttttcaag tatatatttt gctaccacta tggccctttc ctctatatga
    37561 gatctcaaag attacccccc tcacataata gctttaatga tatatatgta aaaacaccaa
    37621 aaaatataac aagcttaacc aaatatgatt aaacttgtta tacttttact taattagact
    37681 cattgggtct atagattttt ctccactata cgcctctaaa tgaacatgta taccttcttc
    37741 actttctatt tgagaagtac tacctacagt acctagagat tgtccttctg ttaccttctg
    37801 ttctttctta acactaacat ttttatctag atttgaatat actactacaa tgttattgtc
    37861 actttttatt ttgactgatt gcccatattc ttcatcatta aatacatcta ctactattcc
    37921 gtttaataga gattttactt ctttgccttt agtacaacta atatctacac ctttatgagt
    37981 ttcccaaaca tcaagagttt tagaataact aggttctttt tctgaatatc ctcttattac
    38041 tttatctcca atgtaattta attttgttgt actcgattta ttttcctctt ttgacttagc
    38101 tttttctaag ttttgttttg aatccgttgc agtagggata gcatctttct tgtctttttc
    38161 aattaaatgt atttcctctt cactatcttt attagcattt tccataattc ctttattaga
    38221 agccaattta tctacattat tatttgtaaa ccaaactcca cctactgcta ataaacaaac
    38281 acatacaaac aaagataaat aaaaaccatc tttttctaac agctttttct tcattttgca
    38341 cctccaaata tatttctaca tttggattat tgacgtgttt ttggcatttt atacaattat
    38401 tagtatatat cttttatttt tgtatctgtg tagtaatgtg ataatatatc ataatattta
    38461 tagccctctt ctgccattcc ttctgctccc cactggctca taccaacacc atgaccatat
    38521 ccttttacca cgaaatctat gtatccttct ccaaatttta tgtcaaagtt tgcagagttc
    38581 aatttaaaaa cagttctaat gtcttttcca gtcaattctt tatttcctaa ttttattttt
    38641 tctacagttc cggcatcact tcttttagtt attgaaactt gtttacttaa attattcaca
    38701 tctatcacaa tagtgctata tgcccttcta agacttttaa caaaatctgt gttacttatt
    38761 ttaagtgttg acgcaaattt aggggaatac ttatcataag gactttccac agactttaaa
    38821 taaggatatt ttgcagaaaa tacttcttca ctattttctg tttttcctga agatgtagaa
    38881 aaataaagag gaagtatcgc cttatcatta taagttatta tttgaccttt agttcctcta
    38941 actgcttctt ggattttgga atatttattc ttaatccatt cttcaccatt taatttttta
    39001 agagtatcat aactcttata ttcttgacaa tgtttataat cagtacatac tactgcattt
    39061 ttatgtttac tagatttacc atgttcctgt ttatatacta catatgttct agctgctaca
    39121 gattgagctt ttaaagcttc tatatcaaac tctgaagaca tctcaccagc tagtactcca
    39181 cataaatagt tctccatgtc catcttttcg gttttccctg ttatatgatt atatacattg
    39241 ataattggtg cttttttatt tacagtttca tagctcactg tacttttttc ttctttatta
    39301 cctttgtctt caatatcact ttttttaata gttttgttta tagatactgg gctctctggt
    39361 ttttcagtta attctacatt tttataagaa actaaagtta taagtgatgg aactaacaca
    39421 gagcaagtca caaatcctaa caaaacaacc aatgggttct tcatatactc tcctccttag
    39481 cttttaaaac cttgtattta agtatatgaa caaccctatt aatttataac tttcttctat
    39541 aaaccaatta ttaatcttct actatttcga tttgaccacc tagtgctgtt atcttcttat
    39601 ctatatcaac ataacctctt tggatatggt atatttctcc tatttgagtt tctccttctg
    39661 ctataagacc gcataaaata agggctgccc ccgctcttaa atcagtagcg tttactgctg
    39721 caccgtgtag ctcatctaca ccattaacaa ccgcacttct tccatctatt tttatattag
    39781 cacccattct attaaattca gcaacatgca taaatctatt ttcaaaaaca gtttctataa
    39841 ctacaccagt tccattggct acagtaagca tagccataaa ttgtgcttga acatctgtag
    39901 gaaatcctgg atgtggtaaa gtttttatat ctattggttt taatactttt ggacctacaa
    39961 ctcttacaga attgtccatt tctgttattt cgcaaccagc ttctcttaat ttagctacaa
    40021 ctggttttaa atgctccatt aaaacatttt caacagttat atcacctttt gtcatagctg
    40081 ctgcaaccat ataagttgct gcttctattc tgtctggaat tacattatgt tctgctcctt
    40141 ttaactcttt aacacctttt atttttatag tattagtacc tgcaccttta acatctgctc
    40201 ccatttcgtt caagaagttt gctaaatcta ctatttctgg ctcttctgct gcattctcta
    40261 ttattgtagt cccttctgct aaagaagctg ccatcattat gttttcagtt gctccaacag
    40321 atgggaaatc taaatataat ttatttccta ctaacttttc tgttgctgct tcaacaaaac
    40381 catggtccat ttctatcttt gcacctaagg ctttaaatcc ttttaaatgt aaatctatag
    40441 gtcttgttcc tatagcacat cctcctggca tagaaatttt cgtactatta aatctagcaa
    40501 gcaatggtcc cataaccaaa aaagaagctc tcatctttct tacaagctca tatggagctt
    40561 cacaagtttt tatattgctt gcatcaacag taagtgtatt ttctttatat tcaacttctg
    40621 ctcctacatg tcttaataaa tctgatataa catgaacatc tcttaaattt ggtactccat
    40681 ttaatgttga ttttccgttt gctaataaag tcgctgctat tattggtaaa actgcatttt
    40741 ttgcaccatc tattttaaca ctacctttaa gtggattact cttttttact attatcttag
    40801 ccatattttt tgatctcctc ataattaata atctaatttt ataattggtg tggcaatata
    40861 tatgagtgtt tttccgtcat cctcattata tttaattcct atatttaaat taattttttc
    40921 ccctaaatat tctaattcat aatcatttaa tactttacta tatgctgtta tagataaaaa
    40981 gtttacgtct ttaacttcat ctatttgctt agcactcata ttatataata ttttttgtaa
    41041 aatatcatca tatttgttgt tttgtaacct ttttgtgtat tctccaacaa tacaagcaga
    41101 cacatctact tgagatgaat atttatttaa cacattttct acgttactat aaataacctc
    41161 tttatgttta tatactttgt tatccaatat gtcaactact atgtaacttt ctttgttatt
    41221 tttatttatt ccttttatag aaatttcttt atcgctacct tttatagtag cacataattg
    41281 tattttatcg ccagaccctt tttgattcca atttattcct tttttatcca actttagatt
    41341 gttaattatt tctataaaaa tattatgaaa ttctttattt gataaattat gatttataac
    41401 agaattaatt ttaacattat aaaatttaaa atttgcttct gatatttcaa gagctttact
    41461 taatctattg tatctagtat ttatacttgt atctgcataa gatattgtca ttcctataat
    41521 cattaatata aaaaagctta tagctaattt tacttttttc atagtaaaaa atccccctta
    41581 aatatgtttt tctgtataat agaattatta acaaatttaa gagagatata ctttctcaat
    41641 atgtattttt atacattttt gtatttttca tctaataatc tctttcaaac tcttcaatct
    41701 caattttctc tatccttctc ctggacttgc caattcctta tatgtatcaa atacatcttg
    41761 cattaattct tatatattat cttctgtaat ataaattact tagtctatac ttaaatctat
    41821 aatctataat ctataatcta taatctataa tctataatct ataatctata atctataatc
    41881 tataatctat aatctattca tattttacca gcaaaataaa cagataatac ttttttatca
    41941 atttccatag ctccaaatga acatacctaa gtacacttcc caatacacaa tttacacact
    42001 cttcctaata aatcaaaaac acctttgttt taaacaactc tattgtactt tcaaaaactt
    42061 tatttgtgtt ctcccacttc aattaaattt gaattatcaa gactccagcc catccatcca
    42121 tcactataaa gggaggtatt ttccactcct atgacatttg catatgttaa tacttcagca
    42181 gcacgccatc cacttccaca cataaatata agctgtttat tcacatctat attttccttg
    42241 tcccacattt ctaaaatctc agatttattt ctcattgtat tatcaatatt acgatattct
    42301 tctagtgaaa cactattact acctgaatgt ccatataaag ctcctggtat acgccctttt
    42361 ttatcataat atgtatatcc agatactttt cctatatgtt catcccaagt acgattatct
    42421 actagtataa acttattttt ttcttttagt ttttgtctta attcagatgt agtaacaact
    42481 aattgtgagt taactggtat atctgctcca aaattagtac atgaatgttt tggattacta
    42541 ataaattcta attcatatcc tgctgataac catgagtttg ttccaccatt caaaactctt
    42601 acatctttta ctcctatata tctcaatata acagctagtc tataagatgc cataggtgta
    42661 atacttgaaa ctattacagt atcatccttt gttagtccat agtctaaagc aaacttagtt
    42721 aagttatcat cattatcaag catccatgtt ggaggaggtt ctataatatc agtatttaca
    42781 tgaacacttg taggtatatg cccttttgta tacgattctt ctccccaact cgcttcaatc
    42841 atttttatat tctttgaatc ttcaaatgtc tcaggaattt ttccatctaa tatatctttt
    42901 attataaaag caggaactat catttgataa tttttatatc tttccattgg aagattttca
    42961 tcgtctgccc attgtttaat atcatattta tatagatatt tatatccttt tttagataaa
    43021 taatctgcta caaccaaagc atcttttcca tttgcatcat acaatactat atttttatct
    43081 aaatcaattc ttttagtttt caatgcttgt tctagcactt catcttttct atctgagtat
    43141 acatttaacc aattagcaga aaaatctact gctcttttta tatgcccacc tctttttact
    43201 ccatctaact tccagccatt ataagcatca tttaagcgag tatcaactat aacccaagcg
    43261 ttatcttgca ttttttcttg taaaacttca gtagatattg ccttaagatt aacaaaataa
    43321 tcttctttct tttctttttt aaaatcattt gtcataattt atactcccaa tttaaaaaaa
    43381 tttttatttg aaattatttt actaaaataa agtaattttc ttatactcta ttagcttcat
    43441 tatattcttt tataaatagt tccttatatt cttattttaa actactcact ttatcttatc
    43501 acaaggttac taataccttg tattttaatt tttctcataa gctattattt ttttatgtct
    43561 gatattgact atatgtttat taagtactca ctatatttta tatgaactat atttatcgtt
    43621 ccctatacct ggtatggtct atatatttat cattttacag atgatttaat atcaatcttt
    43681 ttataaaatt tattatagca aaaacaaaac aaatttaata tttatctaaa aaactcatcc
    43741 tataaaatac aaataacagg atgagttttt ttcaataaat attatctatt taatttttaa
    43801 tctgttttta ttcaactgtt actgatttag ctagatttct tggcttatct acatcgcatc
    43861 ctcttagtac agagacatga tacgcaagta attgcattgg tacaactgct aatattggtg
    43921 ataaaatatc atctgaattt ggtatataga ttattttatc agcagctttc tcaacatcct
    43981 tattatggct ttgagctatt gctactacat aagctcctct tgctcttact tcttccatat
    44041 tagaaaccat tttttcaaat aacttttctt gagtagctat ggcaattaca ggagttcctt
    44101 tttctattaa agctattgtt ccatgcttta gctctcctgc tgcaaaagct tctgcatgta
    44161 tatatgaaat ctcttttaat ttcaatgaac cttccattgc taagctatag tctatacctc
    44221 ttcctaaata gaatgcatgt tcagaactaa caactgtttt agcaacttct tttatatatt
    44281 cttcattgtc taatatttct tgtatttttg atggtatttc tttcatctta gaaatcatac
    44341 tatcataaaa ttctctagtt attgttcctt ttttgatagc aaagtctaaa gctatcatgt
    44401 ataaagaagt tatttgagta gtatatgcct tagttgatgc aactgctact tcaggtcctg
    44461 cccaagtata gaatacatca tctgattctc ttgctataga tgaacctaca acgttagtta
    44521 ttgaaagtat tcttgctcct ttagcctttg aatctcttaa tactgctaga gtatctgctg
    44581 tctcaccaga ttggctaact aatattacta atgaattttc atctacaaat gggtcgctat
    44641 atctaaattc tgatgctata tcagttatta ctggtatatt tacaaatttt tctatggcat
    44701 acttaccaag taatccagca ttatatgctg ttccacaagc cactatataa actttattta
    44761 ttttttctaa gtcttcttta gaaatattta tactgtctag aattatattt ccattatcat
    44821 caagtcttct ttctaaagtt tctctaactc ctgttggttg ctcatgaatt tcttttgaca
    44881 taaagtagtc atatccacct ttagacgcag cttctacatc ccaagttaca tgaaatactt
    44941 ctttttcaac aagatttcta ttagagtcat aaactgttac attttcgtct tttagatgaa
    45001 ctacttctcc attttctaag aaataaacat ctctagtata ttttaataat gcaggtatat
    45061 ctgatgctat aaagttttca ccttcaccaa caccaactac aagaggacta tcttttctta
    45121 ctgcaactaa ttcatttcca tgttctttac atataacacc taatgcataa gctcctctta
    45181 attttgatat agttttgtat acagcatcta ataaattacc ctcatagtat ttatctacta
    45241 aatgtgctat tacctctgta tctgtttgag attcaaactt aacaccttca gaaattaatt
    45301 cttcttttat ttccatatag ttttctatta tcccattatg aacaactgct attgttttgg
    45361 cttgattaaa atgaggatgt gagtttacat cagatggttc tccatgagtt gcccatctcg
    45421 tatgccctat acctaaattt ccatcaatag gattcttttc taagtcttct gcaagtacag
    45481 aaagtctacc tttaaacttt ctaatattaa gttcttcttc attagaacta ttaactgcta
    45541 ctcctgctga gtcataacct ctgtactcta gcttagaaag cccttctact ataacttctg
    45601 ctgcctttct acttcctaaa tatccaacta ttccacacat tatttgtgtc ctcctaaaaa
    45661 tattatttat atattttaag agttaggtca tataagttta ctttggcttc cctttgtcca
    45721 taggattgct cctattattt aaagaagtct ttcgatggta aacacaccgc agagcatccg
    45781 ccgacaattc gattaactct gacctcgtca acttagttcc tatattactc aataattatg
    45841 tctataacta agttctggcg cttagaaagg tgtcctcagt atttgtatct gagaataccc
    45901 tttaaattac gataattttt cttgtataag attagctaag tttgttgcta attcttttat
    45961 ttgaccttcc tctttaccct ctaacataac tctaactaat ggctcagttc ctgatggtct
    46021 tatcaaaact ctaccatttc cgtttaaaat agattctatt ctttctatct cagtttttat
    46081 ttctggatat tccatatatt tgttcttatt ttcatttttt attgttgcat ttactaatac
    46141 ttgtgggtat tggctcatta ttgatgcaat ttctgataaa ggtttctttt cttgcaagat
    46201 tatatttgca agtattagag aactaagcac tccatctcca gtagtgttgt aatctaaaaa
    46261 tatcatatgt ccagattgct ctccaccaag attatatcca tttttaacca tatcttctaa
    46321 aacatatctg tctccaactg ctgtagtaga taaatttatt ccattttcct tagcagctat
    46381 tgtcaaccct atattactca taactgtaac taccaaagta tcttgcgcca atttattatt
    46441 tttctttaaa tttaaggcac tcaatatcat tatatggtct ccatcaacaa tttgaccctt
    46501 ttcatttact gcaattaatc tatcagcatc tccatcatat gcaagaccta agtcagcatt
    46561 atgttccaaa acagcccttt gaagttgttc tggatgtgta gagccacatt tatagtttat
    46621 attatttcca tctggtgaac tatttattga aattacactt gctcctagtt catcaaacac
    46681 tattggtgcc actttatatg acgcaccatt agcacagtcc aaaactactt ttaatccctt
    46741 aaagtcagtg cttattattg attttaggta gtctacatag tctctttgtg cacaatgttc
    46801 atgtaatttt cttcctacat tttcaccaat tggaagacaa tcaattttgt ctatatcgtc
    46861 tatatattct tctattttca attcaatttc atcatctaat ttatatccat tcttattaaa
    46921 gaatttgatt ccattatatt caacaggatt gtgagatgca gatataacca caccacaatc
    46981 agcaccatat tttctagtta aatatgccac tgcaggtgtt ggtattattc caacagtaat
    47041 aacatcacat ccaacagaca taagtcctgc tattaaagat gcttccaaca tatcacctga
    47101 tattctagta tccttaccta ctactacttt tactctgtgg tctccttgag ctaagacaaa
    47161 tccaccagct ctaccaagtt tatatgctaa atcacatgtt agttctgtat ttgctactcc
    47221 tctaactcca tctgttccaa aatactttct cactaaagat tctcctttca taattgaaaa
    47281 gcttgtaatt tgtattatat ttttatagtt cataaaatat cattataggc ttaatttcag
    47341 tcataaaaac attttaatac ttatcagcaa aaaagacaat agaaataatt ctattgtctt
    47401 ttttttgaca tctttttctt tattagttag ttgctttaga acatgtagca tccatacctt
    47461 tttgtaaagc ttctctgttt aatggaacga atttttcttt tctaggtccg aaaacttttt
    47521 tgaatgcttc tagtacagat tctatatcta cagtatcaga tacttttaag taagcgccta
    47581 acattatcat attagcaact ttatcatttc ctaattcagc tgctaattca tttgctggta
    47641 tataataaac atctacatct gttctatcaa ctttttcttt tattaaagaa ctatttacta
    47701 ttatttttcc accaggaata acatcatctt tgaatttttc aaatgctggt atatttaata
    47761 atatagctgc agttgcatca tctgttataa gtggagaacc aactggctca ttagatacag
    47821 ttactgcaca gtttgctgta cctccacgca tttctggtcc ataagatggt aaccaagaaa
    47881 cttcttttcc ttctagcatt cctgcataag taagtaattg tcccatagac ataacacctt
    47941 ggcctccgaa gcctgcacat attactctag ctgtagacat ttacttcacc tcctcaactt
    48001 caacattttt gaaatttcct aatggatagt atggtatcat gttgtctctt aaccattgta
    48061 aagcatcgtt tggagctagt ccccagtttg taggacaagt agataatact tctactattc
    48121 caaatcctaa tcctgcttgt tgaacttgga atgctttctt tatagctttt ttagcttgtc
    48181 taacatgagc tggagtatca actgctactc tttctacaaa tacagctcct gttaatgtag
    48241 ctaacatttc acttactctt attggataac cttgagtttc agcatttctt cctgattgag
    48301 cagtagtagc tctttgtcca actaatgtag ttggagccat ttgtccacca gtcataccat
    48361 aagttgtatt gtttacaaat atagtagtta ttttttctcc tctagcagct gcatgaacta
    48421 tttcagcagt accaattgaa gctaagtctc catcaccttg ataagtgaat actgtatttt
    48481 caggatgaac tctttttatt cctgtagcag cagctggcgc tctaccatga gcagcttctt
    48541 gagtgtcaca gttaaaatat ttatatccta aaactgaaca tccaactgga acaacaccta
    48601 cagcatcacc taatactcct agttcttcta atacttcacc aactaatcta tggattatac
    48661 catgtgtaca tcctggacag tagtgagttt gagtatcttg taatccttca gttttcttaa
    48721 atacaacagc cattatctag cacctcctgc aaccaattct ccggcaataa tttttttagc
    48781 tttttcaact atttctgctg gtgttggagt cattcctcct ggtcttccat ggaagtaaac
    48841 tggtaattta ccttctactg ccattttaac atcttccacc atttgtccca tactcatttc
    48901 aacagtcaat atgtttctag cattaggaat ttggttgaat gcttcgaatg ggaatggcca
    48961 taatacttta ggtcttataa gacctgcttt tattccttct tctcttaata tatcaattgc
    49021 gctttttact actcttgaag tagttccata agctgcaaat acaaattcag catcttctgt
    49081 tttgtacatt tcgtattgta cttcattctt ttccattgca tcaaattttt cttgtaaatg
    49141 ccaacaatgg tcttctagta cttctggttc taagtataga gagtttatta cattaggttt
    49201 tctttttcct ttagttccaa cagttgccca atctttagga ggcaattctc ttttcttctc
    49261 aggagctcta aattcaactg gctccatcat ttgacctatc ataccatctg caactaccat
    49321 aactggtgat ctgtaataat ctgcaacgtc aaaagcttcc attatcatat caacagcttc
    49381 ttgaactgta gctggtgcga atactggagt tctgtagtct ccatttcctc cacctcttgt
    49441 agacataaag tagtcagctt gtgaaggttg tatacttcca agtcctggac cacctctcat
    49501 tacattaagt acaacacaag gaacttctgc tcctactgca tatgttatac cttcttgctt
    49561 taaagcaact cctggtgaag atgaagaagt cataactctt gctcctgcac ctgctccacc
    49621 ataaaccatg tttatcgcag aaacttctga ttcagcttga acgaacgctc cacctatttt
    49681 aggtaactct cttgataaat attctggtaa ttcactttgt ggagttattg ggtaaccgaa
    49741 gaaatatttg caaccagctt ctatagctgc ttttccaaat gcttcgttac ctttcataag
    49801 tatcttagcc attaaaattc ccccttaaat attaattaaa ttaatctctt tctactgtta
    49861 taactacatc tggacacata gttgcacaac ttgcacatcc tatgcatttt tccatttcaa
    49921 aaacagttgc tgggttatac ccttttttgt ttattacatt tgagtctaat tgtatgattt
    49981 ttactggaca tgcttcaaca cacaatccac aacctttaca acgttcttga ttaaaagata
    50041 cttttccttt agccatttac ctaagcctcc ttaaactttc tatatatttg agctcaaact
    50101 acatccagtc ttctctcata tataatttta taggaaaaat ttctccttct aaatcttttg
    50161 gtaactgttc aacaagattt tctaaacatg caacatatct tatcggtata ttacattttt
    50221 cagaaacttc ttttgcaact ttctgacctc ttaaaacatc atcaatagta gtaaacctaa
    50281 taagatgagt attattaatt agacctgtta cctttaattt agaagatttt tctatttctt
    50341 ttatatattg gataacttct tcagaagttt gagttttctc cctatttgca tttattacaa
    50401 aaagcatatc ataatcgcct tcttttaaca aatgactaaa tcttcctata accctagcac
    50461 ctacattatc tccacctaaa tctataacag aattgtatga atggtctacc ataggagaca
    50521 ttacctctgc tgatactgct ggtaaatcta atgttggagc attaattgag ctatctattg
    50581 gttgtatacc taaggatttc attaactctt tcttttctct agttctaaag tatacattta
    50641 ccacatctaa atcggctata gcaactttgc cagaaaccat atctcttaac ttaacaacat
    50701 agttcatcgc aaattcactt ttaccacttc catagtgacc agttatgatt cttattcttt
    50761 tatcattagt tatcataaac ttgagcctct tcttcaccag ttaaaactct aagtccacct
    50821 tgagctaatg caatcatttc atcttcacct ggataaactt ttacatctgc tataaattta
    50881 actctatctg caatcatttc tgtaaacatt tttgaatatg cgattccacc agttaataat
    50941 attgctttta catctccctt aagaactgca gcactagctc ctatttcttt agagatttga
    51001 tatgccatag cttcatatac taatttagct ttttcatcgc cagcttcaat tctttcttca
    51061 acttctctag catcattagt gtttaagtat gcaactaatc cgccattacc ttttattttc
    51121 tttttaattt catcttgagt atattttcca ctaaagcaca tttttactaa tgcacctact
    51181 ggtagtccac cacttctttc tggagagaaa ggtccttctc catctaatgc gtttgcaaca
    51241 tctactattt taccattttt atgagctcca acagaaactc ctccacccat gtgtgcaact
    51301 ataagattta tatcttcata tttcttgttt atttctctag catatcttct tgctattgcc
    51361 ttttgattta aagcatgtac tacacttgct ctacttattt caggcatacc agaaattcta
    51421 gcaacatctt ctaattcatc tacaacaaca gggtctacta tgtatgaagg aacatttact
    51481 tcttcaccta tttgttttgc tattattcca cctagatttg aagcgtgttc tcctaaaact
    51541 cccactttta aatcttcaat catagcagca cttactgaat aagtaccacc ttttataggt
    51601 ttaagaagtc ctcctctacc tactacagca tctaattcag atgtttttac tccaccttct
    51661 tttagagctt cttctattac ttgtttacga aattcaaatt ggtcagacac cttctcatat
    51721 tttcctattt cttctgaaga atgtcttaaa gttttttcaa atactaaatc ctcattatca
    51781 aatacagcta ttttagttga tgtcgaacca ggatttattg ttaagatttt aaatattttg
    51841 ctcatttgcc tatttccccc ttaaaattat gcctttgctg ccattaaaac acctaaagct
    51901 attgagttta gtttagtttc ttcactgtct gctctagaag ttaatattat tggtgcttta
    51961 gccccaacta taactcctgc attttttgat tttgagaaga atactaaagc tttatataat
    52021 atgttaccac cttcaatatc tggggctaat aatatatcag ctcttcctgc tacaggatga
    52081 tttatacctt tatgtttagc tgcttctaaa gatactgcat tatcaattgc aaaaggtcca
    52141 ccaaccatac aacctttgat ttctcctctt tcatacattt cttctagttc tttagcttca
    52201 actgtatctt tcatttttgg atttactttt tcttttgcgc atattgcagc aacttttggt
    52261 tcacttatat ctaatgaatg tgctactgtg caagcatttt ctatgatttg ctttttagta
    52321 tttgtatcag gagctaagtt catagctgcg tcagttacga aaaataatct atcatatccc
    52381 tctacatcaa atactgctac gtgacttaat acatttccag ttctaagacc tacttcttta
    52441 tttaaaactg cttttagtat tattgatgtg tctactaagc ctttcattac catgtcggct
    52501 tttccttgtg aaactaattc aacagatttt agagatgctt ctgctaaatc ttttatatct
    52561 atcagttcat aattttcgat atccatgtct atgctttttg caatttcttt agtcttttct
    52621 atatctccta ctaaaatggc atttgctatt ttttcttttc tagccatttc aactgccatt
    52681 aaaacttctt tatcttggca acatgctact gatatagttt taggtcctct ttcttttgca
    52741 aacttaatta cttcttcaaa acttctcatg catttccacc tcatcttgaa ttttttgaaa
    52801 tagaactttt tatatctcaa ttaaattata actcgttcag caacgttttc ctagagataa
    52861 ttgaaatatt taattcaaaa tatttattta aatctattta tcagtctttt ttcatatatc
    52921 tagcgctttt tagatttaac aaatatctca ttgtccttat tttataatat aactaaaata
    52981 aacaagatgc aacaaaaaca taaaattaac aaacttaatt ttataggttt ttttgtcgca
    53041 tcttgtaaca actaaatatt cgaaatattt gttcattcca cttcaacaat attaggttct
    53101 atatttattt ttttaaattg atatgttgtt tcatatttta ttttatattt actatcttcc
    53161 tcagggcctt gtgataaatc aatatataat gaaatatctt ttttatttaa cttactcaaa
    53221 tcctccgttg actctacaac aacttttata tctacatcag aaattacatt tggattatct
    53281 atatttgttg ggatatttct taattcaata tctcccgcat tatataaaaa ttctgcactc
    53341 aattgttttg tatcatttaa tttaattgtt atagagctat cctcaagagt tataccctct
    53401 ggaatttcta agctaacttc tttttcttca ccattagcca aatcagctaa gtcaataggt
    53461 tttgtcttaa tataacttat tttgtctata tcttctttct ttccttttat tgtaacttgt
    53521 tccttactta atttatagtt tttcagtaca tcattatctt tattatcatt tagacttact
    53581 cttattggga caattttttc cttcaatagt tctatctttg cagtcacaaa ggatgaactt
    53641 aatgttactc cttcaacctt agttccatca ctatttaatg gtattaattg caatttttta
    53701 gaaaaatcat ctgtctcatt accaacatct agattagctc ttacactttt gacttcttta
    53761 actagagttc ttggacctga tacatcgaca aattccttac taagttcaat tttatttata
    53821 tttctttttg aatttccact tacttctacc tttatatctc ttttttcttt tatgtttttt
    53881 tcaagattta ctactaaaac atcatgctta aattctagag taacaccttg aggaatattt
    53941 gcttttaaat atacttcctt ctgtccttgt attgggtctt caatggttcc atatacatgt
    54001 atatcttctt tcttaacact ttgtaccttt gataaactac cttttatata tacatctgct
    54061 gttaattttt cgtctggata tataacaaga tttttatcct ttaaatcact catattactt
    54121 atagatacag gtatatcttc aaacccttta gtttcttcag ggtcaactac agccataaca
    54181 tacatccata atgcaattgc acttaaaaat gatattattt ttattttggt attatttttt
    54241 agtctattta tcatttaaat ataccacctt tgaaaaaact tcttgtttct gtatttgttt
    54301 ttaaattact acgtaatata ttcatcattc tttctctaga aatatctcta tataacttac
    54361 ctgctttagc aattgatacg ccacctgttt cctctgatac aatcagagtt atacagtcag
    54421 acacctcact tacccctatt cctgctctat gtctagtacc caaatcttta cttaaatcct
    54481 tactttccgt caatggcaaa aaacatgcag ctgcttttac cttagaatct ctaattacaa
    54541 cagctccatc gtgtaatggt gtatttggta taaatatatt tattaatagt tgcctagaaa
    54601 tttcagcatc aataatagtt cctgtattta ttatatcact tattctagtt tctctctcca
    54661 ttatgataag agctcctatt ttttgtctag aaagtgaata taaagcttct acaatttctt
    54721 ctatatttct attgagagtt tcttcagaag tatttacatt ctttccaaat aaattaaact
    54781 ttgctcttcc tatatgttca agtcctgatc taaattctgg ttgaaaaatt ataagagcag
    54841 ctataactcc ataatctaaa gctttaagag atatccaata aacggtatgc aatttaaagg
    54901 tgttactaag ttgagttgct aataaaagaa atattatacc tttaaacact tgttctgctc
    54961 ttgtatcttt tataaacata aatattttgt aaaaaatata cgctactata gatatgtcaa
    55021 ttaaatcata tatactcatt cttaatatta tattaaaaaa tccatttaaa aaagaattta
    55081 tatctaacac agttttatcc tcctgtttga taacttctta attatttaca ctaagtatta
    55141 tactatataa atctaactct atacaaacta tttaactttt atgtaaataa ttctagtttt
    55201 atatttggag attgttaaat aatctctaaa cacattaaaa atatcaactt aataaataca
    55261 tatttcttat taaagttagt ttttactcaa ataactcaaa ccatcaaggc gcgaatacaa
    55321 aatagtactc gcgccttaat gcagttttat gttctaaaat atcttaatat atctttgttt
    55381 aatatatatt aattattata aattctataa tccacttatt aactataaac aaatacaaaa
    55441 tctaaatatt acttatcatc aatattattc cattagattc atttttatat taaagtaata
    55501 acttatttca tatctagatt agataaattt attttaatat cttactataa actatcatta
    55561 aaacttatta tgtataacta gatataatct cctacagtga aaataataca ca
//
LOCUS       DAEPLF010000006        52792 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEPLF010000006 DAEPLF010000000
VERSION     DAEPLF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 52792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52792
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.006"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            215..925
                     /locus_tag="KR314_000333"
     CDS             215..925
                     /locus_tag="KR314_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0326743.1"
                     /translation="MISIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSSGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            919..2235
                     /locus_tag="KR314_000334"
     CDS             919..2235
                     /locus_tag="KR314_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH0326744.1"
                     /translation="MLTNLSIGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIFIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMI
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYIDNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIENND
                     ICSIFANSLDNAIEACMDINNELEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            2247..2387
                     /locus_tag="KR314_000335"
     CDS             2247..2387
                     /locus_tag="KR314_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBH0326745.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            2446..3027
                     /locus_tag="KR314_000336"
     CDS             2446..3027
                     /locus_tag="KR314_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBH0326746.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNISVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNNDQ"
     gene            3104..4882
                     /locus_tag="KR314_000337"
     CDS             3104..4882
                     /locus_tag="KR314_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326747.1"
                     /translation="MNLLNIVESIVSCIKGLARRGINMSSIKVISDFITKIIRKNILF
                     AITVIIINYHLIQRWTHISNRGISFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTV
                     KILCRNNFDYFLGISVAVTRNNFIFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILVFE
                     WFLPLTILSIVISFISVIINNLILKITLSSVFLYLTSTKMLDRVIDIKEPFFKALFKS
                     LNIFEDQSYAYYSDFVGKVNNSAYVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLI
                     VIVVYSILFIGLNYISIDMSTYRDEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNI
                     KDRIVLLNTSNEELTNINMILDEIFDIKYIKVNSENIKFNKKKDLIEVNLKESLEPND
                     EVTLDIEYEGYINVLTSWNQNKYIATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSK
                     NNVYSNLPLLNKTSNFLKKDYIFEGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMD
                     IEEKIKFLIETRNEILGDSKASVDAKKYVDEVDNVMESIKRKKINMILVADLPSNEFL
                     YEDSKGEKISITIYHWLSGNILIIDK"
     gene            4897..5769
                     /locus_tag="KR314_000338"
     CDS             4897..5769
                     /locus_tag="KR314_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0326748.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYIPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINNKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            5770..6558
                     /locus_tag="KR314_000339"
     CDS             5770..6558
                     /locus_tag="KR314_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326749.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYLGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVFYIARKSTVLKKSK"
     gene            complement(6654..7487)
                     /locus_tag="KR314_000340"
     CDS             complement(6654..7487)
                     /locus_tag="KR314_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBH0326750.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPLGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(7555..8184)
                     /locus_tag="KR314_000341"
     CDS             complement(7555..8184)
                     /locus_tag="KR314_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBH0326751.1"
                     /translation="MDENITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMI
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            8495..9154
                     /gene="cprR"
                     /locus_tag="KR314_000342"
     CDS             8495..9154
                     /gene="cprR"
                     /locus_tag="KR314_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBH0326752.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            9365..10423
                     /locus_tag="KR314_000343"
     CDS             9365..10423
                     /locus_tag="KR314_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBH0326753.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            10674..12818
                     /gene="fdhF"
                     /locus_tag="KR314_000344"
     CDS             10674..12818
                     /gene="fdhF"
                     /locus_tag="KR314_000344"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:11088..11090,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBH0326754.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKTWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            12867..13700
                     /gene="fdhD"
                     /locus_tag="KR314_000345"
     CDS             12867..13700
                     /gene="fdhD"
                     /locus_tag="KR314_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBH0326755.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCASVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            13711..13884
                     /locus_tag="KR314_000346"
     CDS             13711..13884
                     /locus_tag="KR314_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326756.1"
                     /translation="MCTKKEEQKCSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            13942..14493
                     /locus_tag="KR314_000347"
     CDS             13942..14493
                     /locus_tag="KR314_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0326757.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            14518..15894
                     /locus_tag="KR314_000348"
     CDS             14518..15894
                     /locus_tag="KR314_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBH0326758.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            15894..16409
                     /locus_tag="KR314_000349"
     CDS             15894..16409
                     /locus_tag="KR314_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0326759.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(16487..17770)
                     /locus_tag="KR314_000350"
     CDS             complement(16487..17770)
                     /locus_tag="KR314_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBH0326760.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSHSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            17958..18380
                     /locus_tag="KR314_000351"
     CDS             17958..18380
                     /locus_tag="KR314_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBH0326761.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSEKNGENIIRDILDGNISSECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            18482..19420
                     /locus_tag="KR314_000352"
     CDS             18482..19420
                     /locus_tag="KR314_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBH0326762.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            19606..21630
                     /gene="ligA"
                     /locus_tag="KR314_000353"
     CDS             19606..21630
                     /gene="ligA"
                     /locus_tag="KR314_000353"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBH0326763.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            21790..23139
                     /gene="rph"
                     /locus_tag="KR314_000354"
     CDS             21790..23139
                     /gene="rph"
                     /locus_tag="KR314_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBH0326764.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEIADEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARAIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            23139..23609
                     /locus_tag="KR314_000355"
     CDS             23139..23609
                     /locus_tag="KR314_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0326765.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            23803..25089
                     /locus_tag="KR314_000356"
     CDS             23803..25089
                     /locus_tag="KR314_000356"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBH0326766.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            25238..25822
                     /gene="clpP"
                     /locus_tag="KR314_000357"
     CDS             25238..25822
                     /gene="clpP"
                     /locus_tag="KR314_000357"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBH0326767.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            25841..27091
                     /gene="clpX"
                     /locus_tag="KR314_000358"
     CDS             25841..27091
                     /gene="clpX"
                     /locus_tag="KR314_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBH0326768.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(27162..27689)
                     /locus_tag="KR314_000359"
     CDS             complement(27162..27689)
                     /locus_tag="KR314_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH0326769.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(27677..28189)
                     /locus_tag="KR314_000360"
     CDS             complement(27677..28189)
                     /locus_tag="KR314_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH0326770.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            28369..30732
                     /gene="lon"
                     /locus_tag="KR314_000361"
     CDS             28369..30732
                     /gene="lon"
                     /locus_tag="KR314_000361"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBH0326771.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFNSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     ARKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTAVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            30722..31333
                     /locus_tag="KR314_000362"
     CDS             30722..31333
                     /locus_tag="KR314_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBH0326772.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(31598..32968)
                     /locus_tag="KR314_000363"
     CDS             complement(31598..32968)
                     /locus_tag="KR314_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0326773.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYALGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDISGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(33143..33258)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            33546..34625
                     /locus_tag="KR314_000364"
     CDS             33546..34625
                     /locus_tag="KR314_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0326774.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKATEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            35017..35697
                     /locus_tag="KR314_000365"
     CDS             35017..35697
                     /locus_tag="KR314_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBH0326775.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            35690..37069
                     /locus_tag="KR314_000366"
     CDS             35690..37069
                     /locus_tag="KR314_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBH0326776.1"
                     /translation="MINIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGVLLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            37240..38325
                     /locus_tag="KR314_000367"
     CDS             37240..38325
                     /locus_tag="KR314_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBH0326777.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            38379..38738
                     /locus_tag="KR314_000368"
     CDS             38379..38738
                     /locus_tag="KR314_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBH0326778.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            38791..39663
                     /locus_tag="KR314_000369"
     CDS             38791..39663
                     /locus_tag="KR314_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBH0326779.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            39664..40107
                     /locus_tag="KR314_000370"
     CDS             39664..40107
                     /locus_tag="KR314_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326780.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYINQNKLVDKSKHTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            40122..40706
                     /locus_tag="KR314_000371"
     CDS             40122..40706
                     /locus_tag="KR314_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326781.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            40959..41996
                     /locus_tag="KR314_000372"
     CDS             40959..41996
                     /locus_tag="KR314_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBH0326782.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            42077..42442
                     /locus_tag="KR314_000373"
     CDS             42077..42442
                     /locus_tag="KR314_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326783.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINVAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            42447..42764
                     /locus_tag="KR314_000374"
     CDS             42447..42764
                     /locus_tag="KR314_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326784.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            42812..44416
                     /locus_tag="KR314_000375"
     CDS             42812..44416
                     /locus_tag="KR314_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326785.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVVLKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYINVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            44540..46168
                     /locus_tag="KR314_000376"
     CDS             44540..46168
                     /locus_tag="KR314_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBH0326786.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIAKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            46433..47782
                     /locus_tag="KR314_000377"
     CDS             46433..47782
                     /locus_tag="KR314_000377"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBH0326787.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            47938..49017
                     /locus_tag="KR314_000378"
     CDS             47938..49017
                     /locus_tag="KR314_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBH0326788.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            49362..50270
                     /locus_tag="KR314_000379"
     CDS             49362..50270
                     /locus_tag="KR314_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBH0326789.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGVNIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            50275..52644
                     /locus_tag="KR314_000380"
     CDS             50275..52644
                     /locus_tag="KR314_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBH0326790.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRLTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPKDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
BASE COUNT        21643 a         5515 c         9213 g        16421 t
ORIGIN      
        1 attaaaatcg ctaaatatat tcaatatata ttaaaaaatt gtattataat gaaaattaaa
       61 atagattgta aatccaaaaa gtaatactta atgtccaaaa agctaaaatt aaaatctaaa
      121 tagctatttg ctacagaagt atgttaaaat atgtaaaatg aatagaaatt ttatatattt
      181 attaaaaata tataagaaag gatgtttaag aattgtgatt agtataggaa tatgtgatga
      241 tgaactacat tatcgaataa aaataaaaga tatattaagt gaaatattaa gttcatatcc
      301 tataaattat aacatatatg aattttcatc aggagaagag ttactaaata actatcctaa
      361 agatttagac atattaatta tggacataca aatgaaaaca ataaatggta tggatactgc
      421 taggaagata agagagtttg atcataagtt agaaataata tttgttactt catttgtaga
      481 gtttatgcaa gaaggttatg aggtaaaagc atacagatat atactaaagc caataaataa
      541 agaaaaaatt tctaaaagtg tattaccttg tataaatgag atgatgaaaa agagaaataa
      601 ttacttaact ataaatgtaa aaaattatgt agaccgaatt aaaatagatt ctattactta
      661 tatagaaaca gatagaccga atatattgat ttatacacat gatgatatgt atattactaa
      721 aatgagtata tctaaaatag aaaagatatt aaatgaatat ggatttttta gatgtcataa
      781 tagctatata gttaatttaa aattagtaga atcaatgagt ggaagtactg ttatagtaga
      841 tggcaaaagt attccaataa gtaaatatag agttaaaggc ttgaaacttg ctataactaa
      901 tatattaggg gatatagtat gctaacaaat ctaagtatag gattttggaa tattattaat
      961 ttagtttcag gtactataga ttggattata ttttttttag taatccactt agttggaaag
     1021 aggaaaatat ctaatataag atatatattt acaacaataa ttatatttat atttatggca
     1081 tacataaatg tagttaaagt gtttccaaat attaagattc ttctatgtat ggtaatagga
     1141 atatcatttt atataacatc atatgaagat aaaatatata aaagtataat agttagttta
     1201 ttgttttggc taggattaat gattgctgaa ggttctgcag taggtctagt agtatttata
     1261 aataaattaa ataatattaa cataacacaa catgaaaact tatttagaat acaagctatt
     1321 attatgtcaa aggtattttt atttataggt tttatattgt ttaagtattt taaattatct
     1381 ttagatttta aacctaaaga tatgatacta ataggaatac ctatattatc taatatagtt
     1441 agcttattac ttatatttga atataattta agagtcaata caattactac agaaaactta
     1501 tttatattta tatttgcaat tttattgatt ttatcatcaa gtataatact tttaatagta
     1561 atagggaaaa ttgtacaaga cgataaatta aatctagagt atgaacttat aaatgaaaga
     1621 ataaaaacaa actacaggaa ttatgaaaat ataaacgaag ttcatgatag attaaaatat
     1681 gtttatcatg atttgaaaaa ccatatgatt tgtataaaaa attatgacac taaagaagaa
     1741 attatatcat atatagataa cttagagttt cagataagtg aatttgaaca ttttaaaaat
     1801 actggaaata aaacattaga tttaatttta gcagaaaaaa taagtatttg taaaaagtat
     1861 gatattcaaa ttgaagataa cataaatata tctaaattaa actttattga aaataatgat
     1921 atatgttcaa tatttgcaaa ttcactagat aatgcaattg aagcatgtat ggatataaat
     1981 aatgagctag aaaaaagaat agaagttaaa gctacatata taaataaatt tgcaataata
     2041 aaatttataa atactaaggt aaatgatatt aaacttattg ataaacgtat tcaaactagt
     2101 aaggatgatg ataagataca tggaatagga cttgctagta ttaaatatat agtaaataaa
     2161 tatggtggtg aaacaatagt taattactca gataatgagt ttatattaaa aataatgata
     2221 ccaataaaaa gttaggagag tacaaaatga aaaaaatagc gttaaactta cttaaaaaca
     2281 tctcagcatt aagttttggg atagctgtat tatcagcaaa cagtgcaagt tcttgggtag
     2341 cacatcaggc aaaagagcca caagctttac aaaaactaaa aaaataacat taataaaagg
     2401 acctcttctt attaaaggag aggttttaca tgttgaggag gaaatatgtt taaaagatta
     2461 tcatataaat ttgccaatat attagttaat aatgagattg tagaaagtga agattttgaa
     2521 atatatagat atggatttga aactttgatt tatttcatag ttaatatttc ggtagcatta
     2581 tttataggaa ttatttttga tagatttata catacagtta tatttttaag ttgttattgc
     2641 actttaagac agttcacagg aggttatcat gctaggaatt ataaagagtg tacattaaca
     2701 tttgcagtta tttatttgat aactattttt tcagctaata atatagatat aaataaatat
     2761 aagtatttat tagtattatt aatgattatt agtatactaa caatttacaa gttagctcct
     2821 ttagaacata gaaacaaacc tcttagtgaa agtgagaaaa agcattatag aaagactgtg
     2881 caaaaaatac tattcgtaat aatatgttta attattttat gtaaaatact taatattttt
     2941 cagcaatatg tgatttatgc aataatttct atatattgga ttgctatatt aatatatatt
     3001 ggaatgaagg ttaataatga tcagtaagat tataaaacat tttctaaatt aattaatttc
     3061 actaattaat attaatttta aaaaataatg tcaaggtaaa tatttgaact tattaaatat
     3121 agttgaatct attgtaagtt gtatcaaagg attggctagg aggggcataa atatgagtag
     3181 tattaaggtt atatcagact ttataaccaa aataattaga aaaaatattt tatttgcaat
     3241 aacagttatt attataaatt accatctcat acaacgatgg acacatatat ccaatagagg
     3301 aatttcattt aatgattact tatccttgag tgtatatact tgtatgacaa ttagtatttt
     3361 aaccatgtta aatgtaattt tttatatgag aaaaagttat gatactgtaa aaatattatg
     3421 tagaaataat tttgattatt ttttaggtat ttctgttgct gtaactagaa acaactttat
     3481 attttgttgc ataccaatta tatatgctat agcatactca agtttaataa gaacattatc
     3541 aattgtaacg attaaatcaa ctatatttat attggtattt gaatggtttt taccacttac
     3601 tattttatct atagtgatat catttatttc agttattata aataatttaa tactaaagat
     3661 taccttatca tcagtatttt tatatttaac atcaactaag atgttggata gagttattga
     3721 tattaaagaa ccatttttta aagcattatt taaatcactt aatatatttg aagatcaatc
     3781 atatgcttac tattctgatt ttgtagggaa agttaataat tcagcatatg ttatagataa
     3841 gattatacct acactatttg caattacttt aataatagta gcatatctga tattaaatga
     3901 tattagaaag aaacagatta aatcattaat agttatagta gtgtacagta ttttatttat
     3961 tggattaaat tatatatcaa tagatatgtc aacatataga gatgaggaac gtagttatgg
     4021 agagtattta aagaaatcta atattagtga tattaaaata aaatcgcatg atatgaagat
     4081 tgactttaaa agcaagtcaa atataaaaga tagaattgtt ttattaaaca catcaaatga
     4141 agagttaaca aatattaata tgattttaga tgaaatattt gatataaaat atataaaagt
     4201 aaattcggaa aatataaaat ttaacaaaaa aaaggattta attgaagtta atttaaagga
     4261 aagtttagag cctaatgacg aggtgacatt agatatagag tatgaaggct atattaatgt
     4321 gttgacatct tggaatcaaa ataaatatat agctactaaa gatgatatta tgcttccacc
     4381 aggttcttta gcgtggtacc caaaagtaaa tcaatcaagt tgtatggctt ataaaataaa
     4441 tttaagttct aaaaataatg tctattcaaa tttaccttta ctgaataaga ctagtaattt
     4501 tttaaaaaaa gattatatat ttgaaggaaa gtcaaatgat attgcaatat atgcaggtga
     4561 gtttaaagaa gaggtaaaag atgatataaa aataatctat ccatcagata gtaatgtaaa
     4621 tatggatata gaggaaaaaa ttaaattttt aattgaaaca agaaatgaaa tattaggtga
     4681 tagcaaagca tctgtagatg ctaaaaaata tgtagatgaa gtagataatg ttatggaatc
     4741 aataaaaaga aaaaaaatta atatgatatt agttgctgac ttacctagca atgaattttt
     4801 atatgaagac tcaaagggag agaaaattag cataactatt tatcattggc tttcaggaaa
     4861 tatccttata atagataaat aataaaggag gttaatatga taaacaagtt agaaataaga
     4921 aatctaaatt ttaattacaa aaataaaaaa gctctggata atataaattt aaccctaaca
     4981 gatggagtag ttgcattatt aggtcctaat ggtgctggaa aatcaacatt aatgaggtta
     5041 ttagtgactc tttatgaaac ttcaattgga gagatagaat taaataatat aaagtatagc
     5101 aaaaacaatg agaaaatcaa ggcaaatgta ggatatatac ctcaagattt tgatatgtat
     5161 aataatataa atgggcaaga atacttagag tttgtagcta aaatgagagg tgtatctaag
     5221 aatgatttaa agaaacatat acaaaaagta gtatcaaaag ttaatctgga caagtttata
     5281 aataataaaa taggtacata ctcaggtggt gtaaagagac gactaggtat agcacaggct
     5341 ttaataggtg attctaagtt aatagttatg gatgaaccta cagttggtct tgatccagag
     5401 caaaggaatg aatttagaag attgttacca gaaataagta aaaattctat agtattaatt
     5461 tcaactcaca tagtagaaga catacaattt aactgtgaca aattaattat attaaatcaa
     5521 ggcaaaatat tatatgatgg tacaattaat aaatttattg atatggttga ggtgtattca
     5581 gtagtagttt ctaatgatga atttgaatat ttagaacgta atattgatat tattgattat
     5641 agaagagtta aagatggtgt gcaagtaaag tatataggta aaaatacaga gaaacttaag
     5701 gatagcttaa ggatagatac aactttacaa gatgcttata taaacttttt acataaggag
     5761 ttaaaataaa tgtgtatatt ggagttaata aaatttgagc ttaaaaaaat agagcttaaa
     5821 catgtgttaa tatttgtaat tatttctata ttatgcatat ttattacagg catattagat
     5881 tataatgagt taatatttta taatgatgta cctataatgg atacatattc tatagcagga
     5941 tggttaggaa ttataaagat atcagatgtt tttataccaa taatgtcagc aatcacaata
     6001 atttatgtgt tttcaaaaga ttatttgggt aatataaatg agatacttac tttatataat
     6061 aaaaaaaaat ataatcaatt tatagtattt agatggtgtg ttatattagc tatatatact
     6121 gtattattat taatttcaat tacaatagta tatacattta cgtcagtgaa aggttttaac
     6181 atagacaaaa tagattttgt ttatagtgta aagccaatag atgtgttttt aaagacattt
     6241 cctacattat tatggtatac aacatttcca ctaatgatat taacattaac aaaaaataga
     6301 tttacaacaa tagcaatatt aattacatat acatttgctg atatattttt tattttacat
     6361 atgtacccat ttgttagtat gattaatgta aatggatttt atattcaaaa gatgttttat
     6421 atgcctaata atatagatgt tagatttaat gaattaaaga attatttctt tataaataga
     6481 ggttctctag tagttatttc tataataata gttttttata tagcaagaaa atcaacggtt
     6541 ttaaaaaaga gtaaatagtt taatatattt tttataatgt tatatttata tagaatagtg
     6601 actttaaaaa atttaaagcc actatttttt aatatgtttt atctatataa ctattatttt
     6661 tctaggtttt ttaatatttc gtaatagtgt gttgtggcta tttcacttga ctcgcctttt
     6721 ttacaaaaag ataatttaga tagttttgga actacacgtt caacgacaat tttattacct
     6781 atcattttag attcaagtat cttcaatgct tctaaaaatc tcttatcctt ttttgcacga
     6841 ttataaaatg ataaaacgta gacataaagg aataggttat aattacggaa aggatattca
     6901 acttgcataa ataatgtgcc aattccatag tgacaagggc cgagtggttt tttaatagtc
     6961 caatggtcaa gtagaaaatc tacagctttg tcaagttttg attctttatt tagataatta
     7021 ctaaaacgga atgcatttaa tatattaaga gttggtagtg gatttgagta ttcagtttct
     7081 ggacctcgac caaaactaaa tttattgcaa cgccacccac catcagtata ctgtgtatct
     7141 aaaaaatgtt gaaacgtttt ttgcattcta atgtcagatg catatcccat atgacagagt
     7201 acgttagcag catttgcagt atgacaaggg taaatacctc catttggata tagtttgaaa
     7261 cgtccatctt ctcgccatgt gctaaatatc aagtcagcac attctttaag taaagtatca
     7321 tcagggttca cacctaactc taatagataa agtacactat caagagtaga gaaaggagaa
     7381 ccttttatta aacgcttatc aggtgtagtc caatagtctg caccattatc atacctgtgt
     7441 gataatatta tttcaatatc tgataaagat tgattttcta cattcatttt ttaccccatt
     7501 tcatattata ttctattaaa tctatcaaaa actttattat aatattattt atatttataa
     7561 ttgatttatt ttatttaaaa ttgcatttat agctgtatca ggaacaactg ctaaatcttc
     7621 tgcattgaag taataaacta tattagaatc aaataatata tttgaatttg gaggaaattc
     7681 atcatctcca aaccaaagta taaatatcat agatgtatta cccatatatg taaacctata
     7741 agctaaatct cccatgttta ctttttcagc tccaattttc tccataacta acttatattt
     7801 gtctatttga gatgtaaaaa cttctgaaag cctatataga gtcctgttta aaaaatttgg
     7861 ataataaaca ttgcctcctg gtatttcttt aaaagttaca tatttattag ttctagggat
     7921 acctttagca ttgattaaat atctaactat caagatttta agctcaaagt tattgcaaag
     7981 gctaccatta ctattccaaa cttctccact tggatagtct ataatatatt gtctatcaaa
     8041 aaaattgagt gtgaatctat ttaaatcatt attgaaaggt atattactta aattactaat
     8101 tgtatgaggg tctaattctt tgagtttttt tcgaacatag tcaaatggaa cattataatt
     8161 attttttgtt atattttcat ccataatttt ttcctccaat ttaaacaatt aaaagtgtat
     8221 ttataaaaag ttttcttatt attaatttta ttatatattc aaaatattac aataataaat
     8281 tgttaaataa atttttatat ttaagtaaat gcgtgtaaaa tgaatataaa gtttttgaaa
     8341 tataaagttt ttgtatctaa aatattttat ttgtaaaata gtaattaatt attgtaagct
     8401 ataatatgag agtattatgg ttaatgtgaa ttttaaagat attattaaag atataaatta
     8461 gaatactatt aaacggataa atggaggagt tattatgtct aagatattag tagtagatga
     8521 tgagttagat atgttaaagt tgattgaaaa tgtgttgaaa agagatggac atgaagtaaa
     8581 ggtaattagt gatgtagaaa aggctatgaa catggatttt aattatttta atctaataat
     8641 attagatgtt atgatgccta agatggatgg ttttgaagta tgtaaaacta taagaaataa
     8701 ggttgattgt ccaatactgt ttttgacagc aaaaaatatg gaaagtgata ttatgtacgg
     8761 tcttggcata ggtgcagatg actatataac caaacctttt ggtgtaggag aattaagggc
     8821 taggataact gctcatttaa ggcgagaaag tagagaaaaa aagaatttat taactatctc
     8881 aaatgttcaa tttaactttt taggcaaaga agtttcaatt gatggtaaaa aaattaattt
     8941 tactaaaagt gaatatctaa tatgtgaatt tctagcaaaa aataagggac aagtattttc
     9001 aaaagaaaga atatatgagt cggtatatgg ttttgatgga gaaagtgata taagtactat
     9061 cacagagcat ataaaaaata tacgaagcaa attaaaacaa tatgatattt atacaataga
     9121 aactgtatgg gggatagggt ataaatgggt gtaataagta taagtgttgc ttagtgaaaa
     9181 tattatatat atctacaaaa tgtttgtaaa atattaatct atatattaaa atttaattta
     9241 tagattaata ttttttgtaa atgacaaaat tgtaaggtat tttgttataa aatattaaga
     9301 ttcatagtgt atactaataa tatagtattt agtataaatc ttgagcaaag gatggttatt
     9361 tctaatggat agactaaaga atattagaaa gagtttacta cagtatacat ttttattttt
     9421 gttaataggt ataacaattt acctagtgtt taaaacattg gatattaaaa tgctatcaaa
     9481 tgttatggtt atggtagata agaagttttt attcataggt gctttggcaa taatgtgtca
     9541 cataatgctt gaaggagtag taatgaagat aattatagaa agcactcaca aagttaatat
     9601 tagatttatt ggttttaagc tagccattat gggattttat tataatttaa taactccttt
     9661 tgcttcagga agccaaccag tacagatata tgtacttaaa aaatgtaaaa tgccacttag
     9721 taaggcaagt gcagttgtta ccaataagtc tattattttc caaatagtgg ttacttttta
     9781 ttgtacaata cttgtattaa tgaattttgc tatgttagaa aaacaaatga aaatgattat
     9841 gcctctagtg tttctaggga tagccattaa ttcatttgtc ttaattatga taatattagt
     9901 tattttaaac cctaaaaaaa taaaatattt tatgagaatt actataaagc atttatcaaa
     9961 aattaagttt ttaaaatttt tagataataa gattgaatcc atagaaagtt ttatagatga
    10021 ttatagtaaa gcaataaatt tttttgcaaa gaataaaaag gtattaattt caactataat
    10081 agtaactttt attcaattaa gtgcttactt tagtgtttca ttttggatat ataaagcatt
    10141 taatttacaa ggttatagct atatttatat actgacatta caagcatttt tatatatggc
    10201 aatatctcca ataccaacac ctgggaatgt aggagctaat gaattagcct ttttcactat
    10261 attcaaatca gtattccctc aacctttgat gggatatgca gtatttttat atggtggatt
    10321 tatgtactat ttaattctga ttggaagtgg aatatttact gtaattactc actatagaat
    10381 gaaaaataga attggcagaa atattgtttt aagtgaaaac taaataagtt taaagaatat
    10441 atattgagta tattaaaaaa tatgtcataa gaactagaat attttaatta aatggttgaa
    10501 atataaagat tattatgaca tatttttttt gaaatttaag aggaattgtg agaaaattgt
    10561 tgaatttaat agatagtatt gcaaaatatt tatgattttg catattataa aaacactcat
    10621 tatctttaag ataaatttta cttatcaggg gataaatcta aggggggaat tgtatggaga
    10681 aaaaaatttt aacagtttgt ccatactgtg gagctggatg ccaattatat ctagtagtaa
    10741 aagataataa gatattaaga gctgaaccgg ctaatggtag aacaaatcaa gaaagtttgt
    10801 gtttaaaagg tcgttatgga tgggattttc taaatgaccc taaaattcta acacctaggc
    10861 taaaaaagcc aatgataaga aaacaaggtg tactagaaga ggttgaatgg gatgaggcta
    10921 taaactatac agcttcaaga ctaaatgaaa ttaaagaaaa atatggacct gattctatta
    10981 tgggaacagg ttctgcaaga ggacctggta atgaagcaaa ctatataatg caaaaattta
    11041 tgagagctac aataggaact aataatgttg accattgtgc tcgtgtatga catgcaccat
    11101 ctgtagccgg tctggcttac tcattaggta gtggtgcaat gtctaattct ataccagaga
    11161 tagaaaatgc agatgtttta tttatatttg gatataatgg agcagattca catccaatag
    11221 tggcaaatag aatagtaaaa gctaaaaaaa atggtgctaa attaattgtt actgacccta
    11281 gagtaactga atctgcaaga attgctgata tacatctacc tataaaaggt ggaacaaaca
    11341 tggttttagt aaatgctttt ggaaatgtat taatagaaga aggtctttac aataaagaat
    11401 ttgttcaaaa tcatacacaa ggatttgatg agtataaaga aattgttaaa ccatatacag
    11461 ctaaatatgc tgaaaagatt acaggaatcc cagaagagct tataagaaaa gctatgagag
    11521 aatatgcaaa agggaaaaaa gctatgatac tttatggaat gggtgtctgt caatttggtc
    11581 aagctgttga tgtagttaag ggacttgcat caattgctct acttacaggt aattttggta
    11641 gagaaagcgt tggaatagga cctgtacgtg gacaaaataa tgttcaagga gcttgtgata
    11701 tgggagctct tcctaatgta tatcctggct atcaaaatgt aactgatgat aagataagag
    11761 aaaagtttga aaaaacttgg ggggtaaaat tatctcctaa taatggttat agtttaactc
    11821 aagttccaga cttagtacta aaagagaaaa agcttaaagc ttattatata tttggagaag
    11881 acccagtgca aagtgaccca gatgcatcag aagttagaga agcattagat gaattggaat
    11941 ttgtcattgt tcaagatata tttatgaata agacagcact tcacgctgat gttatacttc
    12001 cagctacttc ttggggagaa catgagggag tatatacatg tgcagataga ggattccaat
    12061 tgatgagaaa agctatagag ccacaaggag atgtaaaacc agactggcaa attataagcg
    12121 aaatttctac agcaatggga tatcctatga attataaaaa tactaaggaa atatgggacg
    12181 aacttagaca attatgtcca agtttcttag gtgctactta tgaaaagata gaaactcaag
    12241 gatgcgttca atggccttgt aagagtgaaa gtatggaaga taaaggaact atgtatctgt
    12301 atgaaggtca aaagttttct actcctaatg gtaaaggtaa tttgttcgct gctgaatgga
    12361 gaccccctat ggaagttgaa gatgatgaat acccatttag tttatgtact gtaagagaag
    12421 ttggacacta ttcagtaaga acaatgactg gtaattgtag aacacttagt tcattagaag
    12481 atgaaccagg acgtgttcaa ataaattcta atgatgctga aaaattagga attgaagatg
    12541 atgaattagt tagaattagt tcaagaagag gaagtgtaat aacaagagca acagttactg
    12601 atagagttaa ggaaggtgca acatatatga cttatcagtg gtgggttgga gcttgcaatg
    12661 aacttacaat agcaaattta gaccctatat ctaaaacacc agagtataaa tattgtgctg
    12721 ttaaacttga aaaacttgaa gaccaagaat tagcagaaaa atgtgtaaga gaagagtatc
    12781 aaagtttgaa agacaaaatg acagctacaa atatctaatg atttattaga tttaagattt
    12841 ataaaaggca gaaaggctaa tattttatga acaatatata ctcaaaaact aaattacaag
    12901 aaaatagtaa ggataaagtt tttgaaaata gttatagtct agtggactac aaatatttaa
    12961 attcttttaa tgtagaagta agcaaaataa atgaacatga agtatcaata gataatgaag
    13021 aaataattgc agaatatcca cttagtttta ttttaaatga taaatatgta aatacatttt
    13081 tatgtacccc atataattta gaggaactta tagtaggatt cttgaaaaca aaaggataca
    13141 tagaaaacaa aaaaagctta ttaaaatttg agataaatga gagtgaacgt tttgcaaaag
    13201 taactattca agagaaacaa aaaaatcaag atgagcaaat tatattttta aattcactag
    13261 actatataac atgtgcttct gtaaagagca atataaaaat aaattatgat acgatttaca
    13321 gtataatgga tagaaattta aattcatcag aattatttaa aaatactggt ggggtacata
    13381 gcgtatctgt atttaatttt gataaaaaaa tagtcacttg tgaagatgta gcaaggcata
    13441 atgcaatgga taaggctatt ggattttgtg ttttaaatga cataacatta aaagataaaa
    13501 ttgtggctgt aagtggaaga atatcattgg aaatgatatt aaaagcagct atgactcaaa
    13561 ttcctattgt agtatcaaaa tcagcaccga ctaatttatc aatagagctt tctaaaaaat
    13621 taaatataac gcttattgga tttgtaagag gaagacggat gaatatttat gcaaatccac
    13681 agagagttat caaaaagtga gaataagtat atgtgtacta aaaaagaaga acaaaaatgt
    13741 tcaaagtgtg aaatctgtgg agtattaatt gacgatactg ataaggtgta taataacaca
    13801 tacaatttgt gcaaaaaatg taaacgaaat ataagtactg agaaaatagt gaaatatata
    13861 aatatatatg atgaaagtaa gtagacatat gatgaaagta actagagggt tggaaatata
    13921 caaattgaaa ggtgggataa tatgaaaaat aaaataggct cttttgtaat agcggaccca
    13981 aacaaatgta caggttgtag agcttgtgaa gtagcttgtt ttacaatgca taatcagcat
    14041 aataatgtgg gttatacagt tggaacagtt gatatacctg ttatacctag actttattta
    14101 gtaaaaggtg acaatttctg tatgccaata cagtgtagac attgtgaaga tgctccttgt
    14161 ttaaactcat gtccacaaaa agctatagtc aaagaaaaca atattatgtc tgtaaatgaa
    14221 gaaaagtgta taggatgtaa aacttgtctg ctagcatgtc cttttggagc tatagactta
    14281 cttccacaat atgaagatgg taaagaagta gaacaagtaa ttttagatga aaataagaag
    14341 atagcttata aatgtgactt atgtaaagac aatgaaaaaa tagcttgtat aagtgcatgt
    14401 cctcaggaag cattaaaact tgttactcca atagatgaca aaaaagctaa gaatagacaa
    14461 gctgcactta gtttattgtt aacaaataaa taaaggatac aggggtaggt gacgtttatg
    14521 ataataaata tagataagga tttatgtact ggatgtagag aatgttcaaa ggtctgtcca
    14581 gtaaatgcta ttgaaggtga agagggaaag ccacaagaga taaatttaga tagatgtgtt
    14641 atgtgcggtc aatgtgttca aacttgtaaa tcttatgcct cagttataga tgaaggattt
    14701 gagtttttac aagagaaaaa acaagaaaga gagataccag agtctataaa tgaacctata
    14761 tttgcagcat acaatgtatg tgatatagat aaggtaaaga aagctttaag tgacccaaat
    14821 gtattcacta tggtccaatg tgcaccagca gttagagttg ctttaggaga agatttcgga
    14881 tattatttag gatacttagg tgctggaaaa atggcagcag cacttagagc tttaaatttt
    14941 gatagagtct acgatactaa ttttgcagca gatttaacta taatggaaga aggtagtgaa
    15001 ttaattaaaa gagtgacaga aggtggaact cttccaatgt ttacatcttg ttgtccagct
    15061 tgggtaaaat tcatggaaaa aaattatcct aaattaacta atcatttatc ttcttgtaaa
    15121 tcacctcagc agatgggtgg tgcaatattt aaaacttatg gtgcagagat aaataatgta
    15181 gatgcatcaa agatatttag tgtttctata atgccatgta catgtaaaaa atatgaatgt
    15241 gatagagaag aaatggattc tagtggatat agagatgtag atgtagtatt aactactaga
    15301 gaactagctt atttaataaa agacatggga attgatttta aaaatttaag agaagaaaaa
    15361 tttgatagtc ctttaggtag ttatagtgga gctggaacta tctttggtgt tactggtggg
    15421 gttatggaag ctgctataag aacaggatat gagcttataa ctggagaaag tattcctgat
    15481 gtagaagtaa aacaagtaag aggagaaaat ggttttagaa agtcaactat taaaatagga
    15541 gacttagact taagagttgg agtagtaagt ggattaaaaa atgtaatacc agtgcttgaa
    15601 gacttggaaa aaggaaaatt agatgtagat tttattgaag ttatgacttg tcctgttggg
    15661 tgtgtaagtg gtggaggtca accaaaaatt ttactagaag aatataagga attggcttat
    15721 gaaaatagaa caaaagcaac atacgtgcat gatgaacatt tagcaattag aaaatctcat
    15781 gagaatcctg atattaaaaa actgtatgat gaatatctgg tagaaccatt aggagaaaaa
    15841 tctcatcatc tattacacac tacatattgt ataggaaagg aagtggctaa gtgatgaact
    15901 attttgttat agcagaccct gataaatgca taggatgtag aacttgtatg ataggatgtg
    15961 tagttgcaca ttcagatgaa gatatttttt accaaaatcc agatgaaata aattttaatc
    16021 caaaactttc tgtcataaag acaaaagaag taagtgctcc aatacagtgt agacattgtg
    16081 aagatgctcc ttgtgcaaag gcatgtccaa atggtggtat agttagggtt gggaacacaa
    16141 taaaaataaa tgaagaaaat tgtataggat gtaagacttg tatgttagca tgtcctatag
    16201 gtgcaataga catagttaca ttaaaagatg tagatgaagg aaagctttgc tttagagaaa
    16261 gaatgacagc aaataagtgt gatttttgta tagaatcgcc agaagggcct gcatgtgtaa
    16321 atgtttgtcc aactaaggca tttactatag ttaaggatga ggatatagat agtaaagtta
    16381 aaaataagag aaaattagct atattgtaat ataaaaaaga tggagtttta tttatgatta
    16441 aaagctccat cttttttatt ataatattta tatatctaaa aatcaattat tgcattagtt
    16501 tatctaatct ttctttattc aattttttca ttcctataga agttataata agtaaagtat
    16561 aggcaattat aactgataaa attataatag accaagaaaa tctgtatcca aaattatcaa
    16621 ctaacaatcc aaataatgtt gaacctaatg caaatcccac tgcaaaaatt aattgtacga
    16681 ttccatatat gctaccaaac tccttttgac caaagaaaga gccagttaaa taagcaggac
    16741 caatcatata agcaaataca gaaagacctt tcaaaatggc aaatacaaat gcaaaagtag
    16801 aagatttatc taccaatagt aatgataaag ttgctataag aaccaataca aaagatataa
    16861 taagtggctt tgtttgaccg aatttatcaa acaataatcc tccaaataaa ttaccaatga
    16921 gagcaaataa agcaaatgtg gagcctactg ttcctattag aataggattt agataatttc
    16981 taagataagt tggatattgt acggataatg atgaaacata tataccaaca aatataaatc
    17041 caatagtcat aatccaaaaa tatttaattt ttttagcttc ttttaatgta taaccccata
    17101 ctgaaggaga aattgtatca gatgctttct cttttttacc tttagatttt accttttcat
    17161 taatatcttt aggcatcctg agtatgaata atattacagg tataccaact attaatgcta
    17221 taattccaaa tattagataa gaatgactgt atccttgagt tgctaataag cgaactgcaa
    17281 gtggttgtag aaatatacta ccaatagaac caccagcaaa agcaaagccc aaagcttttc
    17341 ctttgctttc ttcatcaaac caagcactta ttaaaacagg tattcctata gcagatataa
    17401 ttgtagcgcc aatttgaaca atacatgata cagtgtaaaa ttgccatagt tcagtacata
    17461 tagaaaaagt agcaaaagca atactagaaa ttatacaacc taataaatat agtatcttaa
    17521 tattgacttt agaaaataat acacctataa aaggtgatgc aattgctgag gcaatagtac
    17581 caatagtgaa tattagagaa aaagaagcta aactaaagcc ttctccttct acaacataac
    17641 ttataaattg aggctgtata cttgctacaa tactaaatgg aacagcttgt attaacatac
    17701 aaccaagtac tataacccaa gcatttgaaa agtttttaat gctaaattta ttgtttagta
    17761 atgtattcat ggtatctctc ctttaggtta tgatttgtag atattaatct aattattcta
    17821 attttatata ttagttttgg atagcctaac tatttaaatg taatttgggt attaatataa
    17881 aaattaacta taaaaattga ttattttaaa tgtatggggt aaattaaagt atataaataa
    17941 atgaaaggat ggataaaatg tcatttgaaa aaataaataa taataaccta gataaaatac
    18001 acaataggaa gtgtattatg cttgtaaact ttaataaaaa agaattatca ttaattaaaa
    18061 acgtaagtaa ctttgctgga ataagagacc atataatctt aagtgaaaaa aacggagaga
    18121 atataataag agacatttta gatggaaata tatcaagtga atgtgaaaat gggattaaga
    18181 gtaagtctgt aatatttaat aacactccaa gcacacaagt aaatgcattt ttagaaggtc
    18241 taaaaaaagt gagaataaat agacctttta cagctatggt aacagagaca tctattgatt
    18301 ggacattgaa taatttaata gaaaatttag ttcaagaacg taatgctatg tctttaggaa
    18361 aaacaattga acataagtaa atttaagata tcttatagta attttaacta atgtacattg
    18421 agtatcatat tatattttag aatataaaat tttaaatgtg ggtagataga ggagatttga
    18481 gatgaaagtt ttatttacaa taaattatgg aaaagaaaaa tttgataaaa taagagaatt
    18541 aggctatgat gtcatacatt actctgaaga tagtattgaa aataatgagg atgtaaatac
    18601 agcagatgta ctagttacat acaatccatt tgagagatta gatatttcta agatgaaaaa
    18661 tttacaatat atacagacaa ctagtgtagg tatagaccaa cttccaaaag aagaggtatt
    18721 gaagagaaat attaagatag ctaataatag aggcggatat agtgtaccta ttggtgaaag
    18781 tattgtattg tatattttag aaatatataa aaatagtgca aaatttttta aacaacaaca
    18841 aaataaagaa tggaagatgg atttttctgt atcagaattg tatggacaaa gagttggatt
    18901 tttaggaaca ggtactatat cttcagaagc agcaaaacga ttaaaagctt ttggtgtaga
    18961 aatatggggt gtcaatactg atggaagtag taaaaaatac tttgataagt gttttgctac
    19021 tgaagatatg aatattgtat ttgaagaatg tgatatagta gttgtagcaa tgccttctac
    19081 aaaaagtaca gatggaatag taaacaaaga tatgtttaaa cttatgaaag atggttctgt
    19141 atttataaat gtaggtagag gaaatacagt taatcagaaa gatttagaag aatgtgtaga
    19201 gaaatttaga ggggtagcac tagacgtatt tgaaagtgaa cctttaaaca aggacaataa
    19261 gttgtgggag tgcgaaaatg tcatagtaac tcctcataat tcttgggtgt cagataaaaa
    19321 caaagagaga acatttaata tggtttataa taatttgaaa cactatataa tggagaataa
    19381 acctgtagat aatgttgtag acatatcaag aggctattaa aaataaaaaa ttttgataaa
    19441 aataaaaact cttattagtc gtattaaaga tactaataag agtttttttt gtaataaatt
    19501 taaagtcaaa aaagtgatat actaaaaaat atataaatag gagataatta attataggga
    19561 aatttcaaaa taatttaatt ttaaaaatat gaagaggtgc aaaaagtgag cgtaaaagat
    19621 gaaattaata aacttataga gacaataaac taccataatg aaaggtatta taatcaagat
    19681 agcccagaaa tatcagatta tgaatacgat aagttaatga aggagttaat ttctttagaa
    19741 gaagaacatc cagaactaaa gagaattgat tctccaacaa atagggtagg aggtaagcca
    19801 ttagataaat tcaatcaaat tgtacataaa actcctatgt tgagtttatc aaatgctttt
    19861 tcagagcaag atttgagaga ttttgataag agagttcaag aatatgtggg agaaaatgtt
    19921 gaatatgttg ttgaatttaa aatagatggt ctttctgtag gtctaactta taacaatgga
    19981 gagtttgaaa aaggagcaac tagaggagat ggagttgtag gagaagatat ttctcaaaac
    20041 ttaatgacag taaaaagcat acctcttaag ataaatgata aaaaagaact aatagttaga
    20101 ggagaagtat atatatcaaa agataatttt agaaaagtaa atgaacaaca agaagaacaa
    20161 gaacagccat tatttgctaa tccaaggaat cttgcagctg gttcattgag acaattagac
    20221 ccaaaactaa cagcaaagag accattagat atatttgtat ttaatatgga aaatatagaa
    20281 gaatatgatt tagaaagtca cagtgaatca ctagagtact tagaaaaact aggattttca
    20341 gtaagtcaga gttataaagt ttgtaaaagt atagatgaag ttattgagca tattgaatac
    20401 tggacagata acaggggtaa tttagactac gaaatagatg gtatggtaat aaaagtaaac
    20461 aatataaaac aaagagacga aatgggatat acagccaaaa gtcctagatg ggcaattgca
    20521 tataaattcc cagcagaaaa gaagaaaact aaaatagtag atataatagt agaagttggg
    20581 agaactggta caataacacc ttcagcaatt cttgaaccag tcagacttgc aggtactact
    20641 gtaagtagag ctacacttca caatgaagat tatataagag aaaaagatat aaaaataaat
    20701 gatacagtat tagttcaaaa agcaggggac ataatacctc aagttttaga agttataaaa
    20761 gaggaacgta ctggagatga aatagatttt aaaatgccag aaaagtgtcc agtttgttca
    20821 gagccaacaa taagattaga aggagaggct gctgtaaaat gtatcaatat gtcttgccca
    20881 gcccaaataa gaaggggaat tatacacttt gtgtcaaggg atgcaatgaa tatagatgga
    20941 ttgggagaat ctataattac actgctatta aatgaaaaaa ttattcaaga tgtatctgat
    21001 ttatattata taaaaaagga agatgtagtc gacctagaga gaatgggcga aaaatctgct
    21061 gacaatttaa taaattcaat tgaaaaatca aaagaaaatg atttatggag acttataaat
    21121 ggattaggta ttaaatttat aggagttaaa gcagctaaga tacttgcata taactttaag
    21181 gatttagatg aagtaatttc tgcaactagt gagcaactag aagaattaga ggaatttgga
    21241 agtataatgg caaacagcgt tgtcgagttt tttaaggaag acaaaaatat gaacgttata
    21301 aacaagttaa aaaatgtagg agtaaataca aaatcattgg ataatacagg tgaaaaagta
    21361 gaaaaaatat ttgaagggat gaaaatcgta ttgactggta cacttcctac attaaaaaga
    21421 aatgatgcga aagatatgat agaaaaaaga ggtggaaaag ctacatctag tgttagtaaa
    21481 tcgacaacat ttgttttggc aggtgaagaa gctggttcta aacttactaa agcaaatgat
    21541 ttaggaatta aagttataga tgaagagaga tttttagaat tattaaagtt atcatcgaaa
    21601 gatgaagtaa aagctgtatt agagagttaa aaagtcgctt aatttagtag aaaactacta
    21661 taattaatga tagaatagtt cagtaagcta ataagattac gaaaaatgac aataattaat
    21721 atttgacaaa attaaaaatt ataaaaataa atttataaaa aataaaaaat atgagaaagg
    21781 atataaaaaa tgaccagaag ttatgataga agtaacgaac agataagacc tgtaaagata
    21841 actagagatt atacaaagta tgcagaaggt tctgttctta tagaaatggg agagacaaag
    21901 gttatatgca ctgcatcagt ggaagaaaaa gttcctcctt ttttaagaaa tagtggtact
    21961 ggatggataa atgctgaata ttctatgcta ccaagagcaa cacaacaaag aaagataaga
    22021 gatgcatcta ggggaaaaat tgatggaaga acacaagaaa tacagaggtt aataggaaga
    22081 gcaataaggt ctgttgtaga cttgaataaa ttaggagaaa gaactatttg ggtagattgt
    22141 gatgtgattc aggcagatgg tggaactaga actgcatcta taacaggggc atttgtagca
    22201 gttgcagaag caatatataa attatataaa gcaaagacaa taaaagagat gccaataaca
    22261 aattttgtat ctgcaattag tgttggtatt gtaaatggaa tacacatgct agatttatgt
    22321 tatgaagaag attctaaagc acaggtagat atgaatataa taatgactga taagtgtgag
    22381 tttgtagaag tacaggggac aggagaagag aaaccttttt caagagcaga tttaaattta
    22441 ttgttagagt taggcgaaaa agggaataaa gaattaataa ggcttcaaag agaagcttta
    22501 ggtgaaatag cagatgagat attgggtatg gattatggta atgaagtagt gattgctact
    22561 aataatgctc ataagctaga agaaataggt gaaatcttaa aagattttga atataaagtg
    22621 tactctttaa aagatgttga tttagcaggt atagagatag tagaagatgg aaaaacattt
    22681 gagcataatg cgttgataaa agctagagca atagcaaaaa agactaaact tatagcaata
    22741 tcagatgatt ctggcttaga agtagatgct ttaggcaaaa aacctggtgt ttattcagct
    22801 agatatgcag gagaacatgc aacagatgaa gaaaatagaa aaaaactgtt aaaagctatg
    22861 caaaatgtac caatgagcaa aagaaatgca agattcgtct ctgcaatagc agtagttttt
    22921 cctgatggaa aagaatttgt agtaagagga atttgtgaag gaatgatagg atttgaagaa
    22981 aaaggtaaaa atggttttgg atatgatagc ttatttatag taaaaggata tgataagact
    23041 tttggtgaaa tacctagtgt tataaaaaat tcaatcagtc atagagcaaa cgcactaaaa
    23101 cttatgaaac aagagtttat aaaaagggta gtgaagtaat gaaaataggc atagtaagcg
    23161 acacacatat gatgattaaa aatatggaaa aaacaatacc atatttgaaa gaatgtgacc
    23221 taataattca tgcaggagat aactttactg attcaagata tattcatagc atgactaatg
    23281 taggtattat tgcagttaaa ggtaattgtg actttgatgc tgttgaggaa gaagttgtct
    23341 ttgaagtggc aaataaaacc atattcttat gccatggaga taagtatggt gtaaaatatg
    23401 gaactaatat gttagaaaaa aaggcaactg aagttgatgc tgatatagtt atatttggcc
    23461 atacccatac cccatttaga gaaattaaag atggtgtact ttacataaat cctggaagca
    23521 cttcacttcc aagaggagta tcgtataaaa gttttgtcat tatggatata gaagaagatg
    23581 acataaaaat tgaagaaata cgcatataaa ggtgtattta tctaaaaaaa tggcaaaaaa
    23641 cgctcaaaaa attaaatttt atcaaataag tacaacgata ttaatggaac acttgtaaat
    23701 aaaaaaactt atgtgatata attaagtggt tgaattataa atagaatgaa ttataatacc
    23761 gttatcgggt attacataaa tatcactagg aggattagaa ggatgaaagc agaactaatt
    23821 aaaaaagagg gtaataaagt tactttaaaa ataacagtag ataacaataa atttgaagca
    23881 gcagtaaata aggcttataa taaaagcaga aataaataca atatacctgg atttagaaaa
    23941 ggtaaagctc caagaattgt tatagagact caatatggaa aaggtatatt ctacaatgat
    24001 gctatagaaa tattattccc agaagtttat ccagaagcta tcaaggaatt agacatagac
    24061 ccaatagaca atccagattt agatatagaa gaaataagta aagataatgg gttggttatg
    24121 gttcttaatg tagaagttaa accagagttt gaattaggaa actacaaggg tatagaaata
    24181 gctaaggttg agaatactgt aagtgacgaa aatgttgatg ctaaactaca agaaatggtt
    24241 gaaaaaaatg ccagattagt aagtgtagaa gacaaagctt tagaagatgg agatacagct
    24301 ataatagact tcgaaggttt tgaaaatggt gtagcttttg atggtggtaa aggtgaaaat
    24361 tataacttag ttataggttc aaacacattt ataccaggat ttgaagaaca attagtaggt
    24421 aaaaaagctg gtgaagaagt agaagttaat gttactttcc cagaagaata tcactcacaa
    24481 gatttagctg gaaaaccagt tgtatttaat gtaaaaataa atgatgtaaa agtaaaagaa
    24541 ttatctgcac ttgatgatga atttgctaaa gatactagtg aatttgattc tttagatgaa
    24601 ttaaaagcag atgtaagagc taagttagaa gaagaagcta aaaatagagc agatgcagaa
    24661 actagaaatt cagtagtaga aaaagttgct gaaaatacag aaatagaaat accagaagtt
    24721 atgatacaac atcaaataga taatatgtta aatgaactta actaccaatt acaatatcaa
    24781 ggatttggat tacaacaatt actagagatg actggtagaa ctatggaaga attaagagaa
    24841 gagagaaaag aagatgctaa aaaattagtt aagtcttctt tagtattaga agcaataact
    24901 aaagctgaag gaattgaagc aacagaagaa gaatttaagg ctgagttaga aaaaatggct
    24961 tctgcttaca atatggaagt agaaaaaata gaagcttctt taagagatgc tgacaaagaa
    25021 gatataaaag gtcaaataaa aataagaaaa acaatagatt tattagttga taatgcaaca
    25081 atagcttaat aaaataaacg ctaactaata tagttgacgt tatgaaatgt ttagtatata
    25141 atgatgtgca aatataattt agttaaatgc gtataataat ttacagtagc ttaaaaaagc
    25201 tactgtaaat tatatagaat aaagggggta tgaaaccatg gcattagtac ctgtagttgt
    25261 tgaacaaaca ggaagaggag aaagatctta tgatattttt tctagactat taaaagacag
    25321 aataatcttc ttaggagatc aagttaacga tgccacagca gggcttatag tagcacagct
    25381 attattttta gaagctgaag acccagacaa agatatacat ttatatataa attctccagg
    25441 aggaagtata acttctggaa tggctatata tgatactatg caatacatca aaccagatgt
    25501 aagtactata tgtataggta tggctgcttc tatgggagca ttcttgttgg cagcaggagc
    25561 aaaaggaaaa agactagcac taccaaatag tgaaataatg atacatcaac cattaggtgg
    25621 agctcaaggt caagcaacag atatagaaat tcatgcaaag agaattttaa aaataaaaga
    25681 aactttaaat gagatattat ctgaaagaac tggtcagcca ttagaaaaaa ttaaaatgga
    25741 tactgaacgt gataacttca tgagtgcact agaagccaaa gaatatggtt taatagatga
    25801 agtatttaca aagagaccgt agatagagag aggtgttaat atgtctaaat atgaagaaaa
    25861 acgacaatta aagtgttcat tctgcggtaa aaatcaagat caagtaagaa gattaattgc
    25921 aggtccaaat gtatatattt gtgacgagtg tgttgaactt tgtgacgaaa ttattcagga
    25981 agaaatagaa gatactatag atgaagatac tacatcttta ccaaaaccaa aggaaatgat
    26041 ggagattcta aatgattatg taataggtca agaaaaagct aaaaaagctt tatcagttgc
    26101 ggtttataat cattataaga gaatatacag taaaaaatct agtagtaagg atatagaaat
    26161 tcaaaagagt aatatacttt tattaggacc tacaggttct ggaaagacat tacttgctca
    26221 aactctagca agaactctaa atgtaccttt tgcaatggca gatgctacat cattgactga
    26281 agctggatat gttggagaag acgttgaaaa tatactttta aaacttattc aagctgctga
    26341 ttttgatata gaaaaagctg aaagaggtat aatatatata gatgagatag ataagattgc
    26401 aagaaaatct gaaaacccat ctataactag agatgttagt ggtgaaggtg ttcaacaagc
    26461 acttctaaaa atattagaag gtactgttgc aaatgttcca ccacaaggag gaagaaaaca
    26521 tccccatcaa gaatttttaa agatagatac tactaatgta ttatttatat taggtggagc
    26581 ttttgatgga ttagagaaga taattcaaaa acgtggtgga gataaaacac taggttttgg
    26641 agctaaaata gaaagtaaaa aagaattaga tttagggaag ctgtatgaaa aagtacttcc
    26701 agaggattta ttaaaatatg gtataatacc agagtttata ggaagaatac cagttttagc
    26761 tactttagag ttgttggatg aagatgcatt aatgcagata ttacaggaac ctaaaaatgc
    26821 tctagtaaaa cagtataaaa agttattgga gttagatgat gttgagctag aatttgaaga
    26881 aggtgctctt cgtgcaatcg ctaaaaaggc tatagaaaga aatacaggag ctagaggact
    26941 tagaagtata gttgaaagcg taatgatgga aactatgttt gaagtaccat ctagagataa
    27001 tataaagaag gttatagtaa cagagaaatc tgtgaacgaa gactcagtaa atcctatcat
    27061 agtacttaag gatcaagagg aaagtgcata agatgatgaa aaggagcttt aaaagctcct
    27121 ttttttatgt taatatgcta aatataaaat aggtatcatt atcataaaaa tgtgagtaat
    27181 atacctagaa taccaaagac gaatacaatt gcaatggaac caatttttcc tttatataaa
    27241 agataaaatg atgctataaa tgcaattatt ccatataaat cccaattaac ttctttggca
    27301 gatgaaaaag ctgctgataa tatgaatcct agagtaattg gtctaagaac tgtcaataag
    27361 ttttctacct ttttattatt tttaaattta gaaaataact ttgatataat agttagacct
    27421 attaatgcaa aaattgtaac accaatagtt gcagctagag aaccaaagaa gccagcagtc
    27481 ttaaatccta caaatgtagc actatttatt gctacaggac ctggtgtaga ttgtgatata
    27541 gcaagtaaat ccaaaaactc ttgattagtt ataaagtgat atttagatat aaattcttgc
    27601 tggataaatg gtagcatggc ataaccgcca ccaaaactaa atgcccctat tttaaggaat
    27661 attaagaaga gtagtattat ttctgacata gactatatac acctcctcca attattaaat
    27721 aaatagggct tatattgaat acaccaacag ataaaactgc tataattaat agtagtatgt
    27781 tagttttaga ctttggtagt ttcttgaaca tgctcgtaaa agaatacaat ataagagcta
    27841 ttacaacagg cgcaactcct ttaaaaaatc catctaaaat tttagatgat gaatttttaa
    27901 agtatagata cgataagact aagactatac aaaatgatgg taaagcagag cctaaggtac
    27961 atataagaac tcctggtata ccataaagtt tatatcctaa taatacagat atattaactg
    28021 ccagtactcc aggataactt tgagctatgg ataaataatc aataaattct tcctgctcta
    28081 aaagtttttg tttgtttaca agttcatctt gtataagagg aaccatagcg tatccaccac
    28141 caaatgtaaa cgcaccaatt tttaaaaagg ttaaaaacaa tttcaacata atagaaacct
    28201 ccaaaagata aataacatct aagtatacta atattaatat tatatcataa taaaaagata
    28261 atatagtatg atagtattct aagaatttat cattaatgtt gaaaattaac ttgttcaaat
    28321 atataatatt aaaagcaaag caattagact agaaaaggag taatgttgat ggaacaaaat
    28381 tatactaaaa tagatcatga attaccatta attcctctaa gaggattagc tatatttcca
    28441 tatatgatat taaattttga tataggtaga gagatatctc ttaaagcttt agaccaagct
    28501 atgatggatg aagaacttat atttttaact tctcagaaag aagcagaggt agatgagcca
    28561 ggagaagagg atttttatca tgtgggaaca atctgtaaag ttaagcaaat gataaaatta
    28621 ccaggagata cagttcgtgt tttggttgaa ggtgtatcaa ggggtagagt aaagaaaata
    28681 gaacaagaag atgggtattt cagagcagta atagaagaaa tagtatttaa tagtgataat
    28741 ttggatagtg aaacagaagt tgaaattgaa gcatttgtaa gaaatgtatt tgatgccttt
    28801 gaagagtata taaatattgg aaatagggta tcaccagaaa tattaatatc tctagctgat
    28861 atagaagatg tagatagatt tatagatacg atagctgcaa acatatatct aaaatcaagc
    28921 caaaaacaag aaatattaga agaatttgat attagaaaaa gacttgaact tatttattct
    28981 atattattag aggaaataga tatattaaaa atagaaaaga aaataacttt aagagtaaaa
    29041 aaacagatga ataaggttca aaaagaatat tatcttagag aacaattaaa agctatacag
    29101 aaagaattag gtgaagaaga agatataaat tctgaagcag atgagtatag agaaaagctt
    29161 aaaaaaataa aagcacctaa gacaaccaaa gaaaaaatag aaaaagagat agataaattt
    29221 tcaaaaatat cttctatgtc tccagatgta tctgtaagta gaaactattt agatactata
    29281 ttctcattgc catggaataa agagacaaaa gataaattag atataactaa ggcaaaagat
    29341 atattagatg aagaccatta tggattagaa aaagttaagg agagaatact agagtatcta
    29401 gctataagaa ctcttgctaa gtcacttaaa gggcctataa tatgtctagt tggtcctcca
    29461 ggaacaggaa agacatctat agtaaaatca atcgccagag cattaaatag aaaatttgta
    29521 agaatatctt tgggtggagt tagagatgaa gcagaaataa gaggtcatag aagaacttat
    29581 gtaggttcaa ttccaggaag aataattaat ggggtaaaag aagcccaaac aaaaaatcct
    29641 gtattcttat ttgatgagat agataagatg gcagcagatt ataaaggtga cccagcttca
    29701 gcaatgttag aagtattaga cccagagcaa aataaggact ttgtggacca ttacttagaa
    29761 ataccttttg atttatcaaa aatactattt gttacaacag caaatagtct aggtaatata
    29821 ccaagaccat tattagatag aatggaagta atagaagtat ctgggtatat agaagaagaa
    29881 aaattaaata tagcaagaaa atacctactt ccaaaacaga ttaaagaaca tgctttaaaa
    29941 gaaaacttta taaaaataga tgatgaaact ttaagaagta taattaacca ttatacaaga
    30001 gaagctggtg taagaacact agaaagaact attggtaaga tttgtagaaa ggttgccaaa
    30061 aaatacgttg aagacccaac tcttgaagag gttgttataa ataagtctga cctagagaca
    30121 tatcttggaa aagacatgtt taagtatcaa cttgcagaag ttaatcctca aataggatta
    30181 gtaaatggtc ttgcatggac agcagttggt ggagttacac ttgaagtaga agtaaacgta
    30241 cttaaaggta aaggagaaat tgtattaact ggaaaattag gagatgttat gaaggaatct
    30301 gcgaagacag gtatctctta tataaggtca atagtggaca agtttgatat agaccctgaa
    30361 ttttataaga cgaatgatat tcatatacac attccagaag gcgctgttcc aaaagatgga
    30421 ccatcagctg gtataactat ggctcttgca gtaatctcag cacttactaa aagacctgtt
    30481 cctggaaata tagcaatgac aggagagata actttaagag gtagagtctt agcagtagga
    30541 ggggttaaag aaaagttatt agcagctcat agagctggta taacaaaagt attaattcct
    30601 aaagaatgtg aagctgattt agatgaaata ccagaaaatg taaaagaaaa aatggagttt
    30661 gtgcttgtag aacatatgga tgaagtatta gaacaagctt tattaaagag tggtgaaaat
    30721 aatgaaaatt agaagttcag agataactat gagtgctgtg aataaatcac agtatccagc
    30781 agaaggaatc ccagagatag cattggctgg taggtcaaat gttggaaaat catcaataat
    30841 aaatacattg cttaacagaa gaaacttcgc tagaaccagt caaacaccag gaaaaactag
    30901 aactataaat ttttatctaa taaataatga attttatttt gtagaccttc caggctatgg
    30961 atatgcaaag atagctaagt ctgaaaaaga aaagtgggga ggtataatgg aaagatatct
    31021 agaaagtaga caagagttat gttctatatt cctacttgta gatattagac atgagcctac
    31081 agcagatgat aagcttatgt atgaatggat aaagcacttt ggatataact gtgtagttat
    31141 tgcaactaag gcagataaaa tatctagagg acaatatcaa aaacacataa gtataataag
    31201 aaaaaaattg caaatggaaa gtagtgaaaa agtaattcct gtttcttcac taaaaaagac
    31261 tggtgttgaa gaattatggg aagagattgt aaatcaatac aatcaacatg gatatgaaat
    31321 aacagtagat taaatttaaa atgttaaata ttgacataag atttatttga ataataaatg
    31381 taataatagt tagttttaat tatattaata ttaaagcaaa atataggatg ccttaaaata
    31441 gttttaatga actttttttg gcatccttat tgtttataaa caataaatat cagtaatttt
    31501 gctaaatact aataaatttt agtaaggtat gggaaaatta gtataaatta gttcaactaa
    31561 aaaattattg acttatgtag tcagcaataa atagcattta agaaatagat ttatctgttt
    31621 ttctcataaa gaaaacagtt agtataagcc ctataaaaca tactgatgct gcaatccaat
    31681 aagactgatt gctagctata acataggctg atgtgtgact tttaaacttt tgattgtatg
    31741 taagactttg tgcaaaaaaa ctactaaaaa atgctgttcc aagagcaaat gataaattac
    31801 gaatagttgc aagaaaactg cctccgtacc cctgtttttg aggaggaaca tctgctaaaa
    31861 tactactatt gtttggtgaa ctaaatgctc ctagcccaag tccaaaaata actaaacata
    31921 caattaaaag atacaatgga gaatctgctt ttaaaagtgc cattccaact agtgagatgg
    31981 tcataaatga aaaagatgct gttagaatgt tttttgcacc gtgtttatca gaaagtgaac
    32041 ctgcaatagg tgctataagt accataataa gtggatgtac agtcattaca taaccagcca
    32101 tcataggaga taaatttttt aattgttcta aataaaatgg aagtagaaaa gatactgcca
    32161 tttgaggaaa ataacaagct actccaatga tattgcctac agtaaagttt ctatttttaa
    32221 atagaggtag gtcaataaag ctttgttcaa attttacttc tcttaatgcc aacaataaga
    32281 agattataag tgctgttata cctaaataca atcttgtatt acccttctca ttcattgcaa
    32341 gtaatataag cgtagtgcaa gatgcgaaaa gtagaagacc tgaaatatca aagctcttgt
    32401 tttgttcatc caaaacaggt ttaggcagaa atttaatgcc aagaaagact gctataatac
    32461 caataggtat attgataata aaaatagaag gccatccaaa atgttctaaa attatacctc
    32521 caattacagg accactcata tttcctatac caactacagc tccagtgatt cctatagctt
    32581 ttcctttttc atttgctgga aatgcattgg aaactatacc aagcccagta gccattaaaa
    32641 tacttgctcc aactgcctga aagattcttg aagaaattaa tgttgataaa ttgtttgaca
    32701 tactacacag aaaagaacct attgtaaata taaaaaatcc taatgcgtat agacgatttg
    32761 aacctacttt atcaccaagt tttccgaaaa ataacatagt agctgttaca acaagcatat
    32821 aagctgttac aacccactga atttgtgaca tgttaatttt aaagtcagca gcaattgtag
    32881 gatttgcgat atttagtata cttccatcaa gagtagacat aaatacacca atacagatga
    32941 taatcaatac tatccatttt tgtttcatga taacactcca atcttaatag attttataag
    33001 tacgatataa ttgtaaaata aacaacaaaa aaagcacctg tatattaggt accttgaatg
    33061 tgtctaattt catgaataaa tacggtagtt gaaggatttg tatagattac tcatgtaaaa
    33121 ttacataaag gtactcatat actattttct tctaccatcc agattttact gtcggctttg
    33181 gaatatgacc aaattatgcc ataaggctcg cgggctttta ccgccgatag ggaatttcac
    33241 ccgtttatga agaaaaacag atgtattttg taatattatt ttaacataaa attataatgg
    33301 aaacaattgg atttaagtaa tatccagctg tattacaata caaatatttc aatatggaca
    33361 cttaaattgt atttatagat agaataaggt acagtatgag aatctataat aattagataa
    33421 aattaatatg ttattagtat tagatggtaa gttttaaatt tgtcaaaatc agaaagctgt
    33481 aataaaattc ttataatatg aatatcttaa ttaagagata ttgtaaatta tgaggaggcg
    33541 ctaatatgga aggaaaggta aagaaggcat tcccaggtgg aaatacatca caaggctcat
    33601 attctttttt tgactatata attcctgaaa atgtaaaaag agttttttgt ttaaagggtg
    33661 gacctggagt aggaaaatcc tcacttatga aaaaaattta taaagagttt ttgcatagag
    33721 gatatgatat tgatttatat cattgtcctt ctgacccaag ttcgttggat ggtttagtta
    33781 taaagaaatt aggcgtggta cttatggatg caactgcccc acatactatg gacccaaagc
    33841 tacctggagc tttggatgag ataattaatt taggtgatta ctggaataca gaagaattag
    33901 aaaagaataa acaagaaata tcagtttatg ataaagatat tagtaattca tttaaaagag
    33961 catttaagtt tttaaagtca gcagaaccaa tttttagaga catagaagaa aaatattctg
    34021 attgtatgga ttatggcaaa gtaaatttgg tcacagaaga atttataaaa aggttatttg
    34081 atggagttaa aagcacagga aatctaaaaa gagaaaagca tttatttggt tcagctataa
    34141 ctcctgtagg gtgtttagat tatacagaaa acattttaaa agatgtaaag aaagtctatt
    34201 atttagatgg agaaatagga actgggaaaa ctacattatt aaacaaagta tataagaaag
    34261 ctactgaaaa aggtttaaat gtagaagttt atcattatcc acttattcca gagaaaattg
    34321 aaactgtact attaactgat ttagatatag gcataactat aagtacgact tttaaagaag
    34381 aagatacaat tgatttaaat cagtttttaa atagagaaaa actacttaaa tatgaagaag
    34441 atattaaatt tgatgaaaaa gttttagatg atttaatttg ttgggcagta ctaaatctta
    34501 agagagccaa gtctaaacat gatattatag aaagttacta tagtcctaat atgagatttg
    34561 atgaagtagg gaaattaaga gataagttaa ttaaaagaat tttaaaatat gaggaaaact
    34621 tataaacgta tatgtttaaa attaatgtta ataataatat aatttttagt aaattggtgt
    34681 agctttgagg ctatgccatt ttactttttt ttgctaaaaa atgatattaa taatctccaa
    34741 agccattatt tgtttttcat aattattatt tcctttccta atttatgttt ttttataata
    34801 tcacaacaat gtttattgaa agtttatata attttaagaa catctaaaaa tgcatacttg
    34861 atattggtaa attagtgata gtagttcaat gtatgatatt atatactaaa gatgaatttt
    34921 aatatataac tttgatataa taagcatatg taaatgttta atatttaatt aaagaaaatt
    34981 atcatataaa aataaatttg taaggagcaa tacagtatga aaaaacaata tgtgtttata
    35041 atactagcaa gtactatttt aggtatactt gttggaacca tatttagaga ttatagcaat
    35101 gaagaaacag tttatacaag caaagacaag tcaataagaa aagaaataaa aatgacaaga
    35161 aaatctataa aagaattaac caaagagaaa gacaagttag ataaagaaat taattcaatg
    35221 aaacaaaatc aaaaaaattc tgaagaaata tcagaaatag aaacacttag agatagtcta
    35281 tcatattcag atgtaaaggg tagtgggata atattaagta tagatgctat agatgaaaaa
    35341 acagggaata tagctaatag tatagactat aataagattc ttgtaaaagt tataaatgaa
    35401 ttaaaagtaa acggaggaaa atacatagaa ataaatggtc aaagaattaa tcaatattca
    35461 gaagttgtac ttgcaggaag ccatataaat ataaatggag ttcctgtagc acaaccatat
    35521 gaaataaagt gcattggaga taaggagaaa ttatctaatt atgtagataa aggtagtaaa
    35581 tatttagata gcatacaaac caattatcca atgaaaataa gcataaaaac tgaagaaaat
    35641 atctctatga aaaaaatgaa tatacctaat aaattaaagt atataaaggg tgattaatat
    35701 tatattagag gaaagcagtt tgccaatgtt aataccagaa aaacttgcaa agaaatataa
    35761 tgtatttcca gttgagtttc aagatgataa tttagttgta gaaatagaag atgaggatat
    35821 ttatgctcta caggacttga gattagctac aggtaaagaa attatattga agaaggagaa
    35881 aaaagaactt ataagtgaaa agattgaaaa atattataat agctttgaac ttgatgagga
    35941 ttatgcaaaa aaattatttg aaaatctttt agaaaaagct ataaaagaaa atgcaagtga
    36001 tatacatata gaaccattta aagaacattt aatagtgaga atgagggtag atggagaact
    36061 aaaggaagta tcaaagtttt tagttgatgt ctatccatca ttgtctacgg ttataaaatt
    36121 aaaggcatca atggatataa cagaaaaaag gcttcctcag gatggtaggg tggatataaa
    36181 gttaggtgga aatttaattg acattagagt ttcatcaata ccaactgtct atggtgaaaa
    36241 aatagttttg aggatactta atagagatac ttttttaaaa gacaaattag aacttggttt
    36301 ttcagaggaa gctataaaat ctataaatcg tataataaat aaaagagcag gagtattatt
    36361 agtgacaggt cctacaggta gtggaaagac tacaacagta tattccttat taaatgattt
    36421 aaaaggaata aataaaaata ttatgactat agaaaatcct gtagagtata agatggatgg
    36481 cataagtcag attcaagtaa attcaaaagt agggttgagc tttgatgtag gtcttagagc
    36541 gatattaaga caagacccag atataataat ggtcggagaa ataagagatg cagaaacagc
    36601 taagatagca gtaagagcag ctataacagg tcatttagtt ataagtactt tacatactaa
    36661 tgatgcagta tcttccatag ctagattact agaaatgcaa ataccaccat atcttttaaa
    36721 tgcctctctt ataggtgtta tatctcaaaa gttggttaga aaagtttgta atcattgtag
    36781 ccatgagatt atgataaaag ataattttag tggagatgta aatactaagg tagctgtagg
    36841 gtgtgaaaaa tgtaatgata aaggatactt tggaagaaca gcaatttatg aaatcttaga
    36901 aattaatgat gatataaaga catgtataag aaatatggag gattctagta ctattaaaga
    36961 tgtggcacaa aataatggaa tgataacatt tgaagatagc tgtaaaagac taataaatga
    37021 aaaaataacg acattagaag agtgtttagt agtaaatgaa atgatttaac tgaattgtat
    37081 agttaaactt cgtttggtca gttgtcttta tttaatctat tttaattaag atgtttgagt
    37141 ataaattata tttaagtgaa ataaaattaa atgcattaaa ttagaaaata ttaagtttat
    37201 ttaattgtaa ttaatataat ttggaaggta ttatagtaca tgagattatc taaaaatata
    37261 ttttataatt tagaaaagtt ttatttagta ttaaatagaa tcagtgataa agaaattaga
    37321 gtaatttgca aagagatagg catattgctg gaatctggat gtgaaattac aaaaatattt
    37381 gaaatcattg agagtcagtc aagtaaaaaa gtaaagaatt tgcttagtat agtatcaaat
    37441 catattcaaa aaggtaactc aatagcagaa tcatttcaaa taacaggtat attttctaaa
    37501 ttctttataa gtatgataaa agcaggagaa actagtggaa acttagatat aataatgagt
    37561 gatttatcta attattatga taaagaatac aaattaaaaa tgaaaataat aactatttca
    37621 atatatccaa taatacttat aatattatct atcttatcaa tgttgtttat attcgtattt
    37681 gtaataccta attttcaggt ggtttttacc aacaatggaa ttgaaccacc tttaataact
    37741 agagttttaa tgggaatatc tacagtggtt acaaataact tagcttatat aatatttagt
    37801 tttatattgt tttcaatagg aagcatttat ttctttataa caaatgataa tataaaaaaa
    37861 ttgattaatt cgttaaaatt caaaatacct tttataaaaa aaattaatca gttagtagct
    37921 acaacaagat tttcaagggc attgtccgtt ttaattagca gtggaataca aattgtggaa
    37981 gctatagaca tgtcagctag tgttatagac aacgaattta tttatgaaag gttaaagatt
    38041 tctaataatt atataaaaaa aggaaatagc ataggtaaat ctttgaattt agcaaatgtt
    38101 tttccaaaat tatttatttc aatggcaaat acaggagaag aaagtgggaa actagataaa
    38161 agtctaaatg taataaataa attttatgaa aatgaattaa ataacaaaat agagcaaata
    38221 atgaaattta tagaacctat gctaattgta tttatgggat taattattgg cacttttatt
    38281 ttagcaatgg ttgttcctat gtttgatgca attactaatt tttagagaga aaaataaatt
    38341 ttattagatg aaacttttga aaaggagaga atatttaatt gataaatttg ataaataaaa
    38401 aacgaaaagg atttacactt gttgaaatga ttgtagtagt aactatttta ggcgttatat
    38461 ctagtatagc attagttaag tatagtaagg ttcaagaaag tgccaaatta aatgcagact
    38521 atacgaatgc tgctaatata gtaactgcag ctagcatggc aattaatgat gatgaaaaga
    38581 caatagactc tctaagtgta gaaacattga aggaaaaggg atacctaaat actgttccag
    38641 ttcctcagag tacatcaggt aaattcgaac ttgtcataaa tgatagcgga acagatataa
    38701 gcgtaaatat aaattctaaa caattttatc caaaataata ttttatattg attgctgtaa
    38761 gttttaaaat tatttttatt taagaaggag atggctataa ttaaaaaaat tctaaactta
    38821 gaaataaatg atgattatat aaggcttgta tttttaagaa aaagtcataa aaaagttttt
    38881 atagataaat caattataaa agagattaac tttgatataa aaaatgatag taattcgatt
    38941 gagaagatta aatatataga agatgtagtt agatttgtaa aagaggagat tggtaaatca
    39001 aaaatattat ataaaaattt atactttaac attcaaattc aagatatagt cattagaaat
    39061 gttgaagttc taaatactaa gaaaaaaaga gacatattaa gcatgattga atttgaaata
    39121 acacaataca ttccaataaa cataaataac tattcaataa aatataaagt tataaattct
    39181 aaaaatgata aattgaatat acaagtaatt cttttcccaa agagtattat tcaattaatt
    39241 aagaacattt cagaaaatct aaatatgaat ccaaagacta taaatataaa ttttgatata
    39301 ttacaaaagc ttataaactt aaatttaata gagaattttt cagaagaagg ggtatttata
    39361 gaatgtaaaa aaagtcagtt aattataaat ataacaaaag ataaaaaaat aagagaaaca
    39421 tacttgctat caaaagaggg aaaatcatat gagttcataa aaagattatt aagggatttt
    39481 aagtatgttt attactatgg tatagaaaat tgttttattg aagaatactt taaatataca
    39541 gataaatcta ttgaagtaaa tccattaaga ttaaaagata aagtaaaagt agaaaataat
    39601 tttgatgcag atttagaaga caataggata aattatataa atagtatagg aatgattatt
    39661 taagtgaaag tggataaaac aataaatttt cttaatggat ttaaaaaaac taaaccatgt
    39721 aataaaatct ataaaataat aatagtctta attttaacgt gtattttatt taaatcattt
    39781 atgtatgtga aatctataaa tgaacttgaa gattatataa atcaaaataa gctagtggat
    39841 aaatcaaagc acactttacc aacaacagat aaaactagag agaaattttc taaaataaaa
    39901 cttaatgaaa taaaaaaaat atatgaaaca attggaataa atagtattac taaaatatcg
    39961 atagataaaa ataagctaga gatggaaggt gtgtgtagtg atttagatgt tttaaacaac
    40021 ctaaggaaaa taaagtacat aaaatattat tacatagaca gtattgtaag agatggagaa
    40081 tattatagat ttagaataaa aagttagata ggtgattatt tttgagatta aataatagag
    40141 aaaaaaatct acttaaaata ttgttaatat tagttattat attggcaagt gatatagcag
    40201 taagttattt ggaagaaaac ctaactagtt tgaagaatga aaagcaaaat ataataaata
    40261 aaataaataa tcaagataat tatcaaataa aggataataa gcatatagat gataaatata
    40321 tagaaagtgt tgaattaaat aaaaatgaag aattgaaaaa taattttaat aactcatttt
    40381 tagataaaaa aacaaataaa gatatagtgt tattagtaga aaatgaactg agagatattg
    40441 tgaatgtaga atatattaat caagaaagta gtttagataa tcacaataaa aaacaagtta
    40501 atttagaaat aaaagttaat ggagaacttg caaatgtgtt gaaacttgaa ggtgtaatga
    40561 agaagttaag actaaataat aaacttgaaa agatagaaat taacaaatta aaagtcaata
    40621 attttgatgt tcaaaatatt ccaaatgaat ataaacaaga aaaagacaat attgttgaat
    40681 gtaaaatgac agttagagtc atataacaaa aaagaataga taaagtaaaa aactattatc
    40741 aattaataaa attttataga ggaattagtt ttacgataat tcatttctac aagagggatt
    40801 gttgatgatt ttatcgaaat cctaattaaa gttaatatat tgtaccaaaa tataagtgaa
    40861 tttaatagaa tatttactta tactatagat tgattaataa aaaaatggta taataacaaa
    40921 ttgtacttta atttaataat aagggagtgg aagtcatatt gttagggaga aatgagctat
    40981 gcccttgtgg aagtggcaaa aaatacaaga aatgttgttt aaataaagat attgtttcag
    41041 aaagagcatg cagaaaaact gaactttcac aaaaacaata tacagattta tattcaaagt
    41101 tatatgatta ttcaagacag gataagttta aagaagaatg tgaaaaagct aaagaaatgt
    41161 tttatataat gcaaaatgat agtgtaaatg aaaaatttga aagatttttt aatacttatt
    41221 taatacaaga ccacataatg gaaaataaaa aggttatgac agttggtttt cttgaagaaa
    41281 atggtgcaaa cctaagtcaa tcagaagtat caatattaaa aagtatgttt gaatcatacg
    41341 taagtattta tgaggtaaaa gaagtttctg ctggaaaaat agtattaaag gactgtttaa
    41401 gtggaaatga actttgtaca gaagatgtaa aacttctgag aagttttaaa atcggaagtt
    41461 gtatgatagc tagaatagtt gatattgaag gaactaacat attgatagat ataactataa
    41521 gtatatcaaa tgaggttaaa gatataattt taaaggatat tatgaatcta ttcaatcaat
    41581 atcaagatgt atacaaagat atgaagtcgt tcttaattta tcatacacat atactataca
    41641 agtatataca acagctttta gacccaagta tagctgaata tttaaagaga gaaagagata
    41701 aaaacgatac aaaagttaat tcagaaaatg aattagctga agaagaatgt aaagttttag
    41761 atactcttaa agaaaatgta gaaaaagaaa atatagatgc atgtatagag ttttggaatg
    41821 aatataagaa aaatcatgaa aatataaaag gagctgaaaa tggttgggca gctgctgttg
    41881 aatactatgt taaaaaagaa gcaggacaac ctataactca agttcaaata tcaaagaaat
    41941 acgatataag ctctagtaca ctaggaaaga gatacaaaga tttaaaaata tcttaattaa
    42001 ttataaaaag ctttaagtca tagataaaga atctatagta taatatatgt ataatgttta
    42061 gaaagaggta ttaataatga gtcttagttt aggaaatgca attgctgtat caacgaactt
    42121 tataaaatct aacccagctg tacctgtgta caacggagaa gaaatatatg cagttattta
    42181 tcatatagaa ataggatata ataatggtgt atcacctgat accaaacaat atataataat
    42241 agactataaa gagtttgatt tatctatgga taaaaaaaat gaactaataa atgttgctaa
    42301 agagtactgt aaatctaaag gtattgtaga aaacgaaaac cttgaaataa accttttaga
    42361 caatgaagaa gcagagaact tcttagaaaa ggtgtttaat aacatgaagg ttataagagg
    42421 tcttataact aaaaaaggat aaataaatgg actatatagg aatagaaaat ataacacctt
    42481 atgaaaatac atatgaattt agtgtatatg aatatgatga tgaaatcacc ttaggtagtg
    42541 aaaagttata tgtgtgtgaa ttaagggttg tattgattaa agttaattct ctgtatgttg
    42601 aaagattgca taaatcagtt gaagcaatgg tcttagtaaa aaacttgaaa aaggatttag
    42661 ataaaacact tgttgtaaac aaaataaaga attttgtgct agatgagatt tgggtagaaa
    42721 atctagtaaa agagaatata gaagttatat ttgtagaaag ctaggaataa ccctagcttt
    42781 tttatattac aatctaagag aggaaaacaa tatgttaaga gtatctaatg taaaacttgg
    42841 aattgataaa gatatatctt tattaaaaga tgttgtactt aagaagctaa aaataaaaga
    42901 aaaaaactta gtcaaatata gtatatataa agaatctata gatgctagaa aaaaaggaaa
    42961 aatggaattt gtttattctg tagatgtaga agtcaaagat gaaaataaaa tacttaactc
    43021 aaaaataaaa gatgtaacaa aaataaagga aatcagatac ataaatgtac ctgtgggtga
    43081 taaaaaatta aagaataatc ctttggtaat tggtagtgga ccagctggtt tatttgcagg
    43141 tctgctttta gctcaaatgg gttatgaacc aatcatactt gaaagaggtc ttgatgtaga
    43201 cagtagaacc aaagatataa gtgatttttg gacaactaga aaattaaaaa ataattcaaa
    43261 cgttcaattt ggagaaggtg gagcaggaac tttttcggat ggtaaactta caactagaat
    43321 aaaagatata cgatgtagaa aagtattaga agagttagtt cagtttggag caccagatga
    43381 aatattatac tcacataagc cacatgttgg aacagatata ttaaaaaatg tagttaaaaa
    43441 tataagaaat gaaataataa gtttaggtgg tcaagttaga tttaattcaa aagtaacaga
    43501 tatagttgta tcaaatgatt ctatacaatc tgtaataata aatgatactg aaaaaataga
    43561 tacagataca attatactag ctgttggaca tagtgcaaga gatacttatg aaatgttgta
    43621 ttcaagaggt ataaaaatag ttcaaaaacc ttttgcaata ggagctagag ttgaacatcc
    43681 acaagattta ataaataaag cacagtacaa ggatttttat aatcatccta ggttaggagc
    43741 tgcagattat agacttatag agcatgtttc aaatggaaga acagcatata ctttttgtat
    43801 gtgtccagga ggtagtgtca tagcatctgc atcaaatgat tttgaaatag ttacaaatgg
    43861 tatgagtgaa catgcaagag ataaaataaa tgcaaatagt gcatttttag taaatgtaac
    43921 gccagaagat tttaagagtg ataatccact agcaggtgtt catttccagc agaagtatga
    43981 aaaattagct tttgaattag gtggaaaaaa ttacaatgca ccagttcagc ttgttggaga
    44041 ttttataaac aataagactt ctttgaattt aggaagtgtt gagccatcat atagaccagg
    44101 atatacactt acaaatttaa gtgaatgttt gcctagcttc gtaacagaga ctatgagaga
    44161 agcgtttata tctcttgata aaaaattgaa tgggtttgca atgtatgatg ctgttttgac
    44221 gggagtagaa actaggtcat catcaccaat aagaatagta agagatgaaa atactttaga
    44281 atcagtgagt attaagaatc tatatccatg tggtgaaggc gctggatatg caggtggtat
    44341 agttacagca gcagttgatg gtataaaatg tgcagaaaaa ataatacaaa aatacaccaa
    44401 taatacaaaa atttaacata aacttaatga ttttttaata tttaacatga atttaacatt
    44461 atcactccta taatttgata aaatacaata ggttataaaa tttttagata cagtaatctc
    44521 tttacaagga ggaaataatt tgaacattat aataagtcta atgggtggat taggtctgtt
    44581 cttatatggt atgaacctta tgggagaagg tcttcaaaaa tctgctggga caaagctaaa
    44641 aaagataata aaactactta ctagtaacct atttatggga gttttggtag gtacaggagt
    44701 tacagcagta atacaaagtt caagtgctac tactgtaatg gttgttggtt tcgtaaatgc
    44761 aggaataatg actttaaaac aggcaatagg agttataatg ggtgctaata tagggactac
    44821 tgttacagcc caattggtat ctttcgactt aactggtatg gccccagtag cattaggtgt
    44881 aggtattata ctttatttat ttggaaataa gcctagaata aaaaatatag ccgaaatact
    44941 tataggtttt ggtatattat ttacaggtat ggattttatg aagatggcag tggagccact
    45001 aagagactat caaggattta ctgacctact tgttacattt ggtagatatc cactacttgg
    45061 attattattg ggatttggga ttacagcaat aatacaaagt tcaagtgcat ctatgggtat
    45121 gttggttgca ctggcagcag aaggtctagt gccattatca gcagcactgc caattcttta
    45181 tggccaaaat attggtacat gtgtaacttc actgttatca agtattggtg ctaacaaaaa
    45241 tgcacgaaga gcagctatga tgcatctaat cttcaatgtt ttaggaacgg tgatattctt
    45301 aatattcttg aataaaccag tagtaagcat ggttacaagt tgggatccaa gcaatgttgc
    45361 aagacaaata gccaatacac atacattgtt taatataata tctgtattga tacttttacc
    45421 atttacaaat cttattataa aactagctat aaagcttgtt cctgatagag caggtgatat
    45481 agatgaagat gaaactaaaa ctattaaata tatagatgat agaatgatag aaactccttc
    45541 tatagctctt gcaaatacga taaaagaagc acttcgtatg ggtgagaagg cgaaggaaag
    45601 cctaaatgca tctatggaag ccttagtaga acattcaact gaaaaaatag ctaagacata
    45661 tagacgagag agattaatca atgatttaca gaaagctatt ttaaattact tacttaaact
    45721 ttcaaaagca cctttagatg atgattcaag agaagttgtg gatactttgt ttaatacagt
    45781 caatgatata gaaagaattg gtgaccacgc taaaaatata gctgaattat cgcaagttgc
    45841 aatagattca aatatttctt tctcagaaga aggacaatct gaacttgatg ttatgtataa
    45901 tagagttgtt tcagcatata cttatgcatt agaatcaatg agaactgata atgttgattt
    45961 agcttgtaag gttataaaaa ttgaagaaca agttgatata atggagaaat cttgtagagc
    46021 taatcacatg tatagattga acaataatct atgtagtata gaaaatggag ttatttattt
    46081 agacgtaata agtaatttag agagaatatc tgaccatgct gtcaatatag cacaacaagt
    46141 tatagcgaag agattaggaa atgattaaaa ataattaatt taaaataata taaaatttaa
    46201 aatagaggat aaataaaaga gaaactttaa atgatacgtt tagagtttct ttttttgtaa
    46261 atttatttga ctaaaatttt taaatttata catagttata gtatttagaa gaatcgattt
    46321 cctgataata aatgatgaga tatgtatgat tttgtggcaa acttttaaaa aaaacaccta
    46381 tattttgtgt tataatctct ttaaaacgaa tatgctgaaa agagggattt aaatgggaaa
    46441 aataaatttt gattatagta aagctacaga gtttttttgt caaaacgaaa tagatgtcat
    46501 gcaaccatat gtagatgttg cgcacgacat gttacataat aaaactggtt taggaaatac
    46561 ttttttagga tggatagacc ttcctaaaaa ttatgataaa gaagagtttg acagaataaa
    46621 aaaatctgct gagaagataa aatcagactc agatgttctt ctggtaatag gtataggagg
    46681 ctcttattta ggttcgagag ctgctataga tatggtaagt cattctttta ggaatggact
    46741 taaaaaagag caaagaaagg ctccagaagt atattttgta gggcataata taagctcaac
    46801 atatattatg gatttattag atataattga aggaaaggat atttctgtaa atgtaatatc
    46861 taaatcagga actactacag aaccagctct ggcttttaga atatttaaag attacttaga
    46921 gaaaaagtat ggtaaggaag aagcaagaaa gagaatttat gcaacaacag atgcaagtaa
    46981 aggtgcactt agacagttag ctacagaaga aggatatgag acttttgtaa taccagatga
    47041 tgtaggtggt cgtttttctg tattgacagc agttgggtta cttcctattg ctgctgctgg
    47101 acttgatatt gatgctatga tgaaaggcgc aaatgatgct agagaagcat ttcaaaatcc
    47161 tgacttaaaa agtaatgata gttatagata tgctgttgct agaacaattt tacatagaaa
    47221 aggaaaagat gtagagttat tagtgaacta tgaacctcaa ctacattatg taagtgagtg
    47281 gtggaaacag ctttatggag aaagtgaagg aaaagaaaat aaaggtttat tccctgcttc
    47341 tgtagatttc tcaacagatt tacattctat gggtcaatac atacaagatg gaaaaaggct
    47401 tttatttgag acagtactta atgttgaaaa ctgtaagaga aatataacta taagttcaga
    47461 ggaagtagac ttagatggtt taaattattt agcaggtaag actgtagatt ttgtaaatca
    47521 taaagctttt gaaggaactc tattggctca tacagatgga aaagtaccta atttagtaat
    47581 aaatatacct caactagatg agtataattt tggatattta gtttatttct ttgagaaggc
    47641 ttgtggaata agtggatatt tattgggagt taatccattt gaccaaccag gagtagaagc
    47701 ttacaagaaa aatatgtttg ctttactagg caaaccaggt tatgaaaaag aaaaggaaga
    47761 gctagaaaaa agacttaaat aattttaaat agttaaggtt taattaagat taaaataata
    47821 taatgaaatc atcgaaaagg agtttactca aaatagatga tttcattttt tttatgaaaa
    47881 attgggaaaa atatattttg ataatttata ttatattaag atagggagtg gtatgagatg
    47941 tcaagaagaa agaaagggat aagtttagta atattagttg ctataataag ttcaatactt
    48001 agtagttttc taacaataat tttagttaag gacaatttag taagcaaatc tacaggaagt
    48061 tctacgccga tagttgtaaa tgatgatggt aagagtcaaa atatttatca agcagttgca
    48121 gaaaaagcta cgccatctgt tgtaggtatt acaacaacta gtgttgatac tagtaatatg
    48181 tttgcaatac caactgaaac acaaggtgta ggaactggaa tcatagttga ttcaaatgga
    48241 tatatactta ctaattctca tgttatatca gatggtcaag ctacaagtgt aaatgtatta
    48301 ttcaatgatg gttcaacaac ttcaggtaaa gtagtatggt ttgaccaaca acttgattta
    48361 gctatagtta aggttgataa aactggacta acaccagcag aatttgctga tagtgacaaa
    48421 gtaaaagttg gagatatatc aatagcaata ggtaatcctc ttggattaga ttttcaaaaa
    48481 acagttactc aagggataat aagtggatta gatagaacta tacaaacaga aaaaactaat
    48541 atgactggtt tattacaaac tgatgcaagt ataaatgcag gaaatagtgg aggaccattg
    48601 ttaaatcaaa aaggtcaagt aattggtata aacacagcaa aagcttctca agcagaagga
    48661 ttaggatttg caataccaat caatacagct aagagtatag ttgaagaagt aatcaaaaat
    48721 ggaaaatatg aaaaagtaac tttaggcata aaaggtacag atgtatcaaa ttatgaagca
    48781 gcaacaggta caaaattaag tactgataaa ggtgtttatg ttgcagaagt aatatctggt
    48841 tcatcagcag aaaaagctgg agtaaaggta ggggatatta taactaaagt aggagataca
    48901 gatataacag gtatgaatga tttaaataaa aagttgtata ctttctcaaa aggtgctagt
    48961 acaaaaataa cagtaaatag aggcggaaaa gcagtaacaa taaatgtgaa tttctaaata
    49021 tgaccagaaa gaaggagact acatggtaaa gtgtagtctc tatttttatt taatttttat
    49081 agtatataca tctaaattaa ttaaaatgat gtattgttta tagtttattg ctatattttc
    49141 ttataaaaat aaatttaata aatatttaaa tttaatttac aaaaacaata taaatatcat
    49201 aataaaataa aattttttaa tattactaaa aatatataga caaaataata acaaatatag
    49261 tattatgtaa ttgtagaaat gcttttcata ggtatattct atgaagatga attgcataaa
    49321 aatgaaaaaa tggaatggat attatttagg ggtgaaaaat catgaatcca ttagttataa
    49381 acttacaaaa atgtagcata catgatggac cagggataag aagtacagta ttttttaaag
    49441 ggtgtccact tgaatgtgtt tggtgtcata atccagagag tcaaacatat acaaaacaag
    49501 ttttgtataa tgaagaaaga tgttcaaaat gtgaagcatg tataaatata tgccctcata
    49561 aggcaattta caaaggtgaa acaaaaatat gcttagacca agataagtgt gagttttgtg
    49621 aaacttgctt agactattgt gtaaacaatg caagagagat tgttggtcaa gagtattctg
    49681 taagagactt ggttaaagag atagagaagg atagaatttt ctatgaagaa tcgggtggtg
    49741 gagtaacact atcaggtgga gaagtaatgg ctcaagatat ggacttcatt tgtggagtaa
    49801 taaacatgtg caaatcaaag ggaatccatg tagcaattga tacatgtggc tatgcaaagt
    49861 cagaaaatta tgaaagagtt gctaaatgtg cagatttgtt tttatatgat attaagctta
    49921 ttgatgagga taagcacata aaatttacag gaaaatcaaa tgacttaata ctgaaaaatg
    49981 ttaagatttt aagtgaatta ggggttaaca taaatataag aataccatta attgtaggag
    50041 taaatgtaga tgatgaaaat ttagaggtta aaaaaatgat agagttttta aagcctttga
    50101 atatacaagc agtaagtctt cttccatatc ataatattgg taaacacaag tatgataaaa
    50161 tttataaaaa gtatgaagga gaagaactac aaagaccatc agaagaaaaa ttagaggaaa
    50221 taaaaagact gtttgaagca agtaatttca atactaagat tggagggtaa taaaatggca
    50281 agaggaactt ttgagagaac taaaaaatta agagaagaaa gtattaatgc agaaccacac
    50341 atatctattg aaagagcagt acttatgaca gaagcttata aaaaatatga gggaagtgta
    50401 gagattcctg tacttcgtgc tttatcattt aagcattata ttgagaatag aactcttagc
    50461 ataaatgatg gagaattaat agtaggtgaa aaaggtgatt ctcctaatgg agcaccaaca
    50521 tatccagaaa tttgttgtca cacaatggaa gatttagaag taatgcacaa tagagatata
    50581 ataaatttta gcgtatcaga agaagctaga aaaatacata aagaagaaat aattcctttt
    50641 tggaaaaaaa gacaaacaag agataagata ataaatgcaa tgacacctga gtggttagca
    50701 gcatatgaag caggtatgtt tacagagttt atggaacaaa gagccccagg acatacagtt
    50761 tgtggagata caatatataa aaaaggattt ttagatttaa agaaagacat agaagcaaga
    50821 ttaaaagagc tagattttct taatgattta gatgcatata ataaaaaagc agatttagaa
    50881 gctatggcaa tagcttgtga tgcaatggtt atattaggta aaagatatgc tgagaaagca
    50941 agacaaatgg cagaagaaga aacagatgaa gctaagaaaa aagatttatt actaattgct
    51001 gaaacttgtg atgttgtacc agctcacaaa ccagaaactt atcatcaagc tatacaaatg
    51061 tattggtttg ttcatatagg ggttactact gaacttaata tatgggatgc ttttactcca
    51121 ggaagacttg accaacatct aaatcctttc tatgaaagag atgtagaaaa tggaatacta
    51181 gatagagata gagcacaaga attacttgaa tgtttatggg ttaaatttaa caatcagcca
    51241 gcaccaccaa aagttggtat aacattaaaa gaaagtagca catatacaga ttttgcaaat
    51301 ataaacactg gtggaataaa tccagatggt caagatggtg ttaatgaagt tagttatata
    51361 atacttgatg ttatggatga aatgaaattg atacaaccta gttcaaatgt tcaaataagt
    51421 aaaaagactc ctcaaaaatt cttaaagaga gcttgtgaaa tatcaagaaa aggatggggt
    51481 caaccagcat tttataatac tgaagcaata gttcaagagc ttatggaagc tggaaaaact
    51541 atagaagatg caagacttgg tggaaccagt ggttgtgttg aaactggatg ttttggtaaa
    51601 gaagcatatg ttctaactgg atatatgaac attccaaaaa tacttgaatt aacattaaat
    51661 aatggatatg acccaattag taaaaaacaa ataggtattg aaactggaga ccctagaaat
    51721 ttccaatctt atgaggaact atttgaagca tttaaaaagc aattacatta tatgattgac
    51781 ataaaaatag aaggaaatgc tgtgatagaa aatatatgtg caaaacacat gccttgtcca
    51841 cttatgtcta caatagttga tgattgtata gaaaaaggca aggattacca aagaggtgga
    51901 gcaagataca acactagata tatacaaggt gttggtatag gaactataac agatagttta
    51961 acagcaataa aatataatgt atttgataag aaaaaattcg atatggatac attgcttaaa
    52021 gcacttgatg ctaattttga aggatatgaa gctatactta atttagtttc aaataagact
    52081 cctaaatatg gaaatgatga tgattatgct gatgaaataa tgcaagagat attcaatgca
    52141 tattacaatg aggtaactgg tagactaact gtttgtggtg gagaatacag agtagatatg
    52201 ttaccaacaa cttgtcatat atactttgga gaaattatgg gagcaagtcc aaatggtaga
    52261 ctttgtgcaa aaccagtttc agagggaatt tcacctgaaa aaggtggaga tactaatgga
    52321 cctacagcag ttataaaatc ttgtgctaag atggaccaca taaaaactgg tggaacatta
    52381 ttaaatcaaa gatttgcacc atcagtagtt caaggagaaa aagggttaga taatatggct
    52441 aatctagtaa gagcatactt taatatggat ggtcatcata tacaatttaa tgtatttgat
    52501 aaaaatgtac tgttagaggc tcaaaagaat cctaaggatt ataaagactt aatagttaga
    52561 gttgcaggat atagtgacca tttcaataat ttaagtagaa cactacaaga tgagataata
    52621 ggaagaactg agcaaacatt ctaggtttgc ttgtaaataa aacttcttac tatataaaat
    52681 cgtaactgat ataataaagt ataaatggtt tgattccatt tttaaaattt tagattaatt
    52741 atgagctaac tttctaaata ggaagttagc tcgttaagtt taatgaaaaa at
//
LOCUS       DAEPLF010000007        48965 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEPLF010000007 DAEPLF010000000
VERSION     DAEPLF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48965
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.007"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..949)
                     /locus_tag="KR314_000381"
     CDS             complement(<1..949)
                     /locus_tag="KR314_000381"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBH0326791.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIA"
     gene            complement(1191..2513)
                     /gene="dnaA"
                     /locus_tag="KR314_000382"
     CDS             complement(1191..2513)
                     /gene="dnaA"
                     /locus_tag="KR314_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBH0326792.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            3169..3306
                     /gene="rpmH"
                     /locus_tag="KR314_000383"
     CDS             3169..3306
                     /gene="rpmH"
                     /locus_tag="KR314_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBH0326793.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            3384..3728
                     /gene="rnpA"
                     /locus_tag="KR314_000384"
     CDS             3384..3728
                     /gene="rnpA"
                     /locus_tag="KR314_000384"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBH0326794.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            3760..4011
                     /gene="yidD"
                     /locus_tag="KR314_000385"
     CDS             3760..4011
                     /gene="yidD"
                     /locus_tag="KR314_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBH0326795.1"
                     /translation="MNKIIYILKEISRYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            4043..4750
                     /locus_tag="KR314_000386"
     CDS             4043..4750
                     /locus_tag="KR314_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBH0326796.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            4719..5348
                     /locus_tag="KR314_000387"
     CDS             4719..5348
                     /locus_tag="KR314_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBH0326797.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            5460..6839
                     /gene="mnmE"
                     /locus_tag="KR314_000388"
     CDS             5460..6839
                     /gene="mnmE"
                     /locus_tag="KR314_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBH0326798.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGERALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNILDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKEIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            6934..8829
                     /gene="mnmG"
                     /locus_tag="KR314_000389"
     CDS             6934..8829
                     /gene="mnmG"
                     /locus_tag="KR314_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBH0326799.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVDKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQVRR
                     TRGGKGE"
     gene            8822..9541
                     /gene="rsmG"
                     /locus_tag="KR314_000390"
     CDS             8822..9541
                     /gene="rsmG"
                     /locus_tag="KR314_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBH0326800.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGRPAKNPIK"
     gene            9716..10501
                     /gene="noc"
                     /locus_tag="KR314_000391"
     CDS             9716..10501
                     /gene="noc"
                     /locus_tag="KR314_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBH0326801.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            10636..11409
                     /locus_tag="KR314_000392"
     CDS             10636..11409
                     /locus_tag="KR314_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBH0326802.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            11411..12274
                     /locus_tag="KR314_000393"
     CDS             11411..12274
                     /locus_tag="KR314_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBH0326803.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            12307..13449
                     /locus_tag="KR314_000394"
     CDS             12307..13449
                     /locus_tag="KR314_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBH0326804.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVSDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(13583..14158)
                     /locus_tag="KR314_000395"
     CDS             complement(13583..14158)
                     /locus_tag="KR314_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBH0326805.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDTSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            14418..15710
                     /locus_tag="KR314_000396"
     CDS             14418..15710
                     /locus_tag="KR314_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH0326806.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            15815..16417
                     /gene="yedF"
                     /locus_tag="KR314_000397"
     CDS             15815..16417
                     /gene="yedF"
                     /locus_tag="KR314_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBH0326807.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            16490..16753
                     /locus_tag="KR314_000398"
     CDS             16490..16753
                     /locus_tag="KR314_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBH0326808.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            16768..16953
                     /locus_tag="KR314_000399"
     CDS             16768..16953
                     /locus_tag="KR314_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBH0326809.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            17056..18252
                     /locus_tag="KR314_000400"
     CDS             17056..18252
                     /locus_tag="KR314_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0326810.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLEKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            18466..18744
                     /locus_tag="KR314_000401"
     CDS             18466..18744
                     /locus_tag="KR314_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBH0326811.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            18781..19215
                     /gene="ssb"
                     /locus_tag="KR314_000402"
     CDS             18781..19215
                     /gene="ssb"
                     /locus_tag="KR314_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH0326812.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            19234..19461
                     /locus_tag="KR314_000403"
     CDS             19234..19461
                     /locus_tag="KR314_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBH0326813.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            19604..19924
                     /locus_tag="KR314_000404"
     CDS             19604..19924
                     /locus_tag="KR314_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBH0326814.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            19932..20888
                     /locus_tag="KR314_000405"
     CDS             19932..20888
                     /locus_tag="KR314_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBH0326815.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            20899..22893
                     /locus_tag="KR314_000406"
     CDS             20899..22893
                     /locus_tag="KR314_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBH0326816.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            22894..23343
                     /locus_tag="KR314_000407"
     CDS             22894..23343
                     /locus_tag="KR314_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBH0326817.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            23362..24690
                     /gene="dnaB"
                     /locus_tag="KR314_000408"
     CDS             23362..24690
                     /gene="dnaB"
                     /locus_tag="KR314_000408"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBH0326818.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            24895..25203
                     /locus_tag="KR314_000409"
     CDS             24895..25203
                     /locus_tag="KR314_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326819.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            25285..26574
                     /locus_tag="KR314_000410"
     CDS             25285..26574
                     /locus_tag="KR314_000410"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBH0326820.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNKIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            26900..26975
                     /locus_tag="KR314_000411"
     tRNA            26900..26975
                     /locus_tag="KR314_000411"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26933..26935,aa:Lys,seq:ttt)
     gene            26982..27056
                     /locus_tag="KR314_000412"
     tRNA            26982..27056
                     /locus_tag="KR314_000412"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27015..27017,aa:Glu,seq:ttc)
     gene            27061..27136
                     /locus_tag="KR314_000413"
     tRNA            27061..27136
                     /locus_tag="KR314_000413"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27094..27096,aa:Val,seq:tac)
     gene            27170..27244
                     /locus_tag="KR314_000414"
     tRNA            27170..27244
                     /locus_tag="KR314_000414"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27202..27204,aa:Thr,seq:tgt)
     gene            complement(27627..28619)
                     /locus_tag="KR314_000415"
     CDS             complement(27627..28619)
                     /locus_tag="KR314_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0326821.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKEKLLSESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(28628..29686)
                     /locus_tag="KR314_000416"
     CDS             complement(28628..29686)
                     /locus_tag="KR314_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBH0326822.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            29811..31274
                     /locus_tag="KR314_000417"
     CDS             29811..31274
                     /locus_tag="KR314_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBH0326823.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            31376..32173
                     /locus_tag="KR314_000418"
     CDS             31376..32173
                     /locus_tag="KR314_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0326824.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            32186..34288
                     /locus_tag="KR314_000419"
     CDS             32186..34288
                     /locus_tag="KR314_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBH0326825.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEKEI"
     gene            34356..34835
                     /gene="rlmH"
                     /locus_tag="KR314_000420"
     CDS             34356..34835
                     /gene="rlmH"
                     /locus_tag="KR314_000420"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBH0326826.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIVLAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            34984..35295
                     /locus_tag="KR314_000421"
     CDS             34984..35295
                     /locus_tag="KR314_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0326827.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            35343..35660
                     /locus_tag="KR314_000422"
     CDS             35343..35660
                     /locus_tag="KR314_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0326828.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SKEVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            35873..36574
                     /locus_tag="KR314_000423"
     CDS             35873..36574
                     /locus_tag="KR314_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0326829.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            36798..38099
                     /locus_tag="KR314_000424"
     CDS             36798..38099
                     /locus_tag="KR314_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0326830.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKATLDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            38236..39330
                     /locus_tag="KR314_000425"
     CDS             38236..39330
                     /locus_tag="KR314_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBH0326831.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMVSKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAKEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNIFKRGDVVIGNDDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            39332..40465
                     /locus_tag="KR314_000426"
     CDS             39332..40465
                     /locus_tag="KR314_000426"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBH0326832.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNHIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGEFY
                     EDTKKYKTNVYALINIAKQGITSQDELSNMMNIDEYELKNAVRKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEAKERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGNSIEENE"
     gene            40483..41592
                     /locus_tag="KR314_000427"
     CDS             40483..41592
                     /locus_tag="KR314_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBH0326833.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFL
                     IIEELVNKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEINFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDNIYNRIKNIVES"
     gene            41657..42397
                     /locus_tag="KR314_000428"
     CDS             41657..42397
                     /locus_tag="KR314_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBH0326834.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLVGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGDTKVEDIGVLYKIMKELLG"
     gene            42402..43394
                     /locus_tag="KR314_000429"
     CDS             42402..43394
                     /locus_tag="KR314_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBH0326835.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKKIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(43581..44462)
                     /locus_tag="KR314_000430"
     CDS             complement(43581..44462)
                     /locus_tag="KR314_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0326836.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            44735..46123
                     /locus_tag="KR314_000431"
     CDS             44735..46123
                     /locus_tag="KR314_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326837.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPNNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKAGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            46151..47104
                     /locus_tag="KR314_000432"
     CDS             46151..47104
                     /locus_tag="KR314_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBH0326838.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLESGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            47164..47388
                     /locus_tag="KR314_000433"
     CDS             47164..47388
                     /locus_tag="KR314_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326839.1"
                     /translation="MNEKPSFKQIEIILAVIFLVLTYYKIGLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            47381..47893
                     /locus_tag="KR314_000434"
     CDS             47381..47893
                     /locus_tag="KR314_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBH0326840.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDKKKGN"
     gene            47925..48779
                     /locus_tag="KR314_000435"
     CDS             47925..48779
                     /locus_tag="KR314_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBH0326841.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
BASE COUNT        19168 a         5428 c         8720 g        15649 t
ORIGIN      
        1 aagctatatc taggtaatct ccatctaaat ctatttcaac ttcttcatac acatttcctt
       61 tttctgtgtt agaagtaatt gccattactt tatctcttat agataacttt ataagattat
      121 ttttttctga ttgagacaat aaagacgctc tctctatact atttaaaagt tccttagtgt
      181 ttaatttaac tctgctgtta tgttctcttg gcaataattt tttataatca ataaaatctc
      241 cttcaagcaa tcttgtaata attttcgtat cattaattat aaaaatagca tttttttcgt
      301 taaacccaac tttaacatta tcctctccag aaagcaaact atttacatct ataagtgtct
      361 ttccaggaat tatgactttt atattttcag ttaaactatc aactgaacaa ctttttacag
      421 ctaatctata tccatcaatt gctactaaat ttaagtttct atctacaatt tctaaaagtt
      481 ctcccattaa aacaggtttt gtttggtcct gtgagatagc aaatacagtt tgtttaatca
      541 tattttttag aatttcttga ggtatgctat ataaatcttc ttcatttaat tctggtaatt
      601 taggaaattc ttttgctgca taacctttaa ttttaaatct tgaatttaca caatttatgt
      661 aaatattatt ttctgaatca gtttctatct caacaaagga atctggtaat tttctaatta
      721 tatctccaaa tagccttgca tctacaacaa catcaccttt ttctatgatt tctgcttgta
      781 cataagtttc tattcctatt tcagcatcat atccagtaag ttttaattga ccttcttctg
      841 tagatataag tatacctttt aataattcta ttgttgtttt tccattaata gctttttgag
      901 caattccaat tctatttgct aggatttttt gattacaaat tattttcaat gtggataacc
      961 tcctctggct ttttatatat aaaaataagt taaaatcata gtagtaatag tagtaggtgt
     1021 tgttgataat gttaataagt atgtataaca ttataaatac aaagatatta acatgtgtat
     1081 aagatgttaa tatctttgtt attttagact tgataatttg ttaataattt ttgttcaaaa
     1141 aaagtattaa caggttattc aagagatata aacatacaat tgtgtataaa ttatcccttc
     1201 aaatctgata taattttgtc tattttagtc ttaatttctt cactttcttc tatatcctta
     1261 gatactttgt catgagcatg aattactgtt gtatggtctc tacccccaaa ctcttcacct
     1321 attttaggaa gggataaatc agtaagctcg cgcgttaaat acatagctat ctgtcttgga
     1381 taagctattg aacgtgttct ttttttagaa ttaaaatctt ccatctttaa attgaataca
     1441 gaagatactt tttcttttat tctaagaaca tttatttctt catttttaga agttgttata
     1501 atatctttta aagcttctgt agcaagatca aaagatatta ccctattgct aagagaagaa
     1561 taagcaacca ctctagttaa tgcaccttct agttccctta tgtttgactt aatatttttt
     1621 gctatatatg acataacctc atttggcaca tctatacgtt ctagttgagc cttttttcta
     1681 agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat cagttataag tcccatttca
     1741 aatctcgatc ttagcctatc ttctagtgta ggtatgtctt ttggcggtct atcactagat
     1801 ataattattt gtttgtttgc ttcatgaaga gtattgaatg tatggaaaaa ctcttcttga
     1861 gttctttctt ttcctgctat aaattgaata tcgtcgatta gtaatacatc tacgtttcta
     1921 tatttattcc taaattcttc gtttttatcg tcttttatag agtttataag ttcattagta
     1981 aatttttctg aagaaacgta aactactttt gagtcttttt tttgactaac aatatgatga
     2041 cctatagcat gcattaggtg agtcttacct aatccaactc ctccatacaa aaaaagtggg
     2101 ttataagctt tagctggaga ttctgcaact gcaacgcatg cagcatgtgc aaatctattg
     2161 ctattaccaa taacgaatgt atcaaatgtg tattttggat ttagattggg gtaaagaact
     2221 ctataattta aatctgtgct atcagaattt aaatcagcaa cctctttttc gctcaataca
     2281 aatttaatat tatatttttt taatgacagt tgactaatag catcttcaat taaatgtaaa
     2341 tatctattct ctaagatatc tttattaaaa tcactaggag ccagtaagat taaatcatta
     2401 agatgtattt ttaatggtac gatgttttta aaaaaggtat taaaacttac tgaagttaag
     2461 tcacctttta ttaattgtag ggttttgtcc cataaagaaa ctatatccat aatataaacc
     2521 tccgaacaga ataattaata ttttccacaa agttattaac agaaaagcaa aaatatatat
     2581 aaattttaca gaataactaa atttatggat aatggtgttg ataaattaat aatagtataa
     2641 attattaaaa aaatatgaaa agatataaac aatatgtaga taaagttata aataacataa
     2701 acatacaata aatggtgatt ataactagag tattgtcagt aatcttaaaa gttatacaca
     2761 aaagtatcca caagctgtga ataatttcta ataagttgat aacatttatg cacattatca
     2821 acaagtatat ttataatatc aaaatatatg tcaaacttca atgatttgta actaatttat
     2881 ccacaatatt aactaattgt tgataaaaat ataaacatgt taaaattact ttatttgtgt
     2941 atataattga aaaaaataat ttagaataaa attaaataga tttttgctag tattttcaat
     3001 aaaaaaaata cataatatat atatggtaga ttttgtgata taagcgtccc aaaaactcgt
     3061 tgacaacagt agtaaaagta tttataatta tatgtgttat gtttactttg agggtaaagt
     3121 tattattttt ataaacggaa aataaaatct gaaaggcggt gcaaaataat gagtaaaaga
     3181 acttatcaac caaaaaagag acaaagaagt aaagaacatg gtttcagaaa aagaatgaag
     3241 acgtctaacg gaagaaacgt gttaaagaga agaagagcta aaggaagaaa tagattaact
     3301 cattaattac aaaagggccg ctgcaaaggt ggcctttttg tgtaaataat tacaattcat
     3361 agctgtgttt atggaggtta actatggact ttaataggac taaagggttg aaaaaagact
     3421 ctgattttag aaaagtatat aaacacggca aatcttttgc aaacaaatat ttagtaatat
     3481 atatacttaa aaataaatca gattatagta gagtaggtat ttctgtttca aaaaaagtag
     3541 gaaaagctat tactaggaat agagtaagaa gattaataaa agaagcctac agattaaata
     3601 ttgatgaaaa aattaagcct ggatatgata tagtatttat agcaagggta tctagtaagg
     3661 atgctacttt taaagacata gacaagtcta taaaaaattt agttaaaaga acggatattt
     3721 ctatttaatt gtatgctata aatatggagg ttaatgactt tgaataaaat aatttatata
     3781 ttaaaggaaa taagtaggta tttatcgaat ttatgtattt atttagtgag attttatcaa
     3841 aagtatatat ctcccttaaa gggacctacc tgtagatttt atccaacttg ctcacaatat
     3901 tcaatagagg cgtttaaaaa atatggattt gtaaaaggta tgtatttaac gataagaaga
     3961 cttttaaaat gtcatccttt tcatccagga ggttacgacc ctctaaaata aaagagagta
     4021 actattagga ggtataaaaa aattgaatat aataggaaat ttcttaggat tgattcttaa
     4081 ggtaatattt gaatttgtta atcattatgg tatttctata atacttttta cgatacttgt
     4141 aaagattatt ttattaccat taacaattaa gcagactaag tcaacaaagg caatgcaaga
     4201 tatacaacct cgtataaaag agatacaaga aaaatataaa aataagccag aaaaacaaaa
     4261 tgaagaaatt gttaagttat atggagaagc taaaattaat ccactatctg gttgtttacc
     4321 tttattaata cagtttccta tattaatagg attatttagc gttttgagag aaccagttgc
     4381 tcatggcgtt tttgcaaaca aagctgcatt tttagcagct gacaatgggt ttttatggat
     4441 aaaaagctta acatcaccag attatgtatt agcagtattt tcaggagcaa gtgcatatgt
     4501 catgcaaaaa gtaatgacac ccaaagatca gttacaaggc tctatgaaag taatgacata
     4561 tgtaatggca ggtatgtctt tctattgggg atttatattc ccagcaggac ttacattgta
     4621 ctggacagta agtaacttat tctcaatagc tcaatattac ttaataatga atcctttaaa
     4681 agctaaactg gctgctaact ctaaggagga aaaagttaat gagaaaaagc ctaaaattaa
     4741 taaaaagtaa gagtaaagag gaagctataa gtaaagctat agcagagttg aatttaaaag
     4801 cagaggatat agaagtagaa atacttgaaa atccaagtaa agggttttta ggttttatag
     4861 gtgctaaaga tggtacttat gaaatttttg ttatcgagag ggaagtagat gtagctaaaa
     4921 attttataga ggtaatgttg aaaaatgcaa atgttgatgc caaagtcaat gtatctcaaa
     4981 aagataattt aatcatggtt gatattgaag gcaaagaagc tgcaagttta attggtagaa
     5041 gaggggaaac tttagattct attcaatttt tgactggatt agctcttaat aaaataaata
     5101 aagattctca tacaagagta cttgtagata tagaaaatta tagatcaaaa cgagaagaat
     5161 ctttaataag atatgctaat aaagttgcta gagaagtagc gaaaacaaga aaaactaaaa
     5221 aattagatta tatgaatcca tatgagagaa gaataataca ttcagctctt caaaatgaca
     5281 aatatgtaat tacatatagt gaaggaaccg atccatatag aagattagta attgagtgta
     5341 aaagataaag tcaacctcct aaacattagg gggttttatt ttttaatatt attaaatcat
     5401 taatgattgg ttaaatgtaa acctatatgt aaaaaattat gaattaagag gtgataaatt
     5461 tgtttataga tgatacaatt gcagcaatag ctacagcacc tggagaaggt ggtataggta
     5521 tacttagaat aagtggagaa agagctttga aggttgcaga agaaatcttt aaatctatgt
     5581 cgggaaagag tattgaagaa tacaataaga gaacattaat atatggaaat atattggata
     5641 atgaaaatat aattgatgaa gttttattgg catatatgaa aggaccaaac tcctatacag
     5701 gagaagatgt tatagaaatt aattgtcatg gtggatttat ttcggtaaag aagatattag
     5761 aattaatact atcaaaagat gttagattag cagaggctgg tgagtttact aaaagagcat
     5821 tcttaaatgg aagaatagat ttatctcaag cagaggctgt aattgatgtt ataaaagcca
     5881 aaactgatat agctcatgaa gttgctcaaa atcaattaga gggttcttta tcaaagaaga
     5941 taagagaatt gagagataaa gtcactgaga tattagctca tgtagaagtt gctatagatt
     6001 atcctgagga agatattgag catataacat atcaaactct taaagaaaaa actgatgaac
     6061 ttaaaaaaga gattaaaaaa ttatatgata cagctgaaag tggaaaaata cttagagaag
     6121 gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa atcttcacta cttaattcta
     6181 ttttaggaga gaatagagca atagttactg atataccagg aactactaga gatgttatag
     6241 aagagtttgt aaacataaaa ggtatacctt taaaaattgt tgacacagca ggtataaggg
     6301 atactgatga tatcgttgaa aaaataggtg ttgaaaagtc taaagaatca tttactagtg
     6361 cagatttgat agtaatggtt ttagatgcat ctagaaagtt gtcagaggaa gatatagaga
     6421 tattagaaaa acttaaggat aaacaaacaa tagtgctgtt aaataagaat gatttgaaac
     6481 aagagattga agaagaaaaa atacttaaat atgtagaaaa taatagtata ataaaaatat
     6541 cagctttaca acaagaagga atagaagaat tgcaagataa aatagagtct atggtatata
     6601 aaggtagtat caaaaataat tctagtttag tagtaacaaa ttcaagacat aaagatgcac
     6661 ttagtaaagc atataaatct gcaacagatg ctttaattgc tttggaacaa agtatgccat
     6721 ttgactttgt agaagtcgat ttaaaaaata tatgggacta tcttggatat attaatggtg
     6781 atacagtaac agaggattta ctagataata tattccataa tttctgtatt ggtaagtaat
     6841 gtacaaaagt aaatttgttt ttattataga gatttttaaa aaatttgaag tgtttctcat
     6901 gaaacattaa ttttgaaaag aaaggaatta taaatgataa agtttgaagc aggaaaatat
     6961 gatgttatag tagtcggagc aggacatgct ggatgtgaag ctgctttggc tactgcaaga
     7021 atgggatata aaactttgat aataacaatg tctttggatt ccatagcatt aatgccttgt
     7081 aatccatcaa ttggagggac tggtaaaggt caattagtta aagaaataga tgccttaggt
     7141 ggtcagatgg gattaaatat agacaagaca tatatacaaa gtagaatgtt aaatactgcc
     7201 aaaggtccag cggtacactc tttaagagca caagcagata aatttaaata tcatgaagaa
     7261 atgaaaaaga cattagaaga tgaaccaaat cttgatatag ctatggatga agttgtagaa
     7321 atacttcatg aaggaaatgt tgtaatagga gttggaacta aactaggatg ttcatttaaa
     7381 tctaaagctg taatattggc cactggagtt tatctgaact caaagatata tatgggtgaa
     7441 gttgcatttt atgaaggtcc taatgcattg gggtatgcaa aatatcttac agatagctta
     7501 gttgagcttg gtcttagaat gagaagattt aagacgggaa ctccagcaag agttcataga
     7561 gatagtatag acttctctgt aatgtcttta caagagggcg atgaaaaagt aactccattc
     7621 tcatttatga atgaaaatat agaaaaaaaa caagaacctt gttatttaac aagaactaca
     7681 gaagaaactc aaaaagttat actggataat ttaaaaaggt ctgctatgta tagtggagta
     7741 atagaaagta ctggtccaag atactgtcct tctatagaag ataaagtagt aagatttagt
     7801 gataaaacct cacatcaatt atttatagaa ccagaagggt taaatactaa agaaatgtat
     7861 atacaaggaa tttcaactag tttacctttt gaagttcaat tagatatgta caaaactata
     7921 aagggacttg aaaattgtaa gataatgaga cctgcatatg ctattgaata tgattgtgtt
     7981 gacccaactc aactaaaaat aagtttggaa ataaagggtg tcgagaactt atttagtgca
     8041 gggcaattta atggaacttc tggatatgag gaagcagcag ctcaagggct tatggctggt
     8101 ataaatgcag ttagaaagat agaaggaaaa gagccgtttg tattagaccg ttcagaagct
     8161 tatataggtg tcttattaga tgaccttgtt actaaaggaa ctaacgagcc ttacagaatg
     8221 atgacatcaa gagcagaata tcgactatat ttaagacaag ataatgcaga tatgagatta
     8281 actcaaaaag gttatgatat aggattagtt aaaaaagata gatatgaaag gttcctaaat
     8341 aaaaaggctg ctgtagataa agaatttgaa agattaaaaa atgaaagagt tacaccaaaa
     8401 gaagtaaata gtttgttaga agaaaaagga gcaacaccaa taaaggttgg aatatctcta
     8461 tatgaatttt taaagagacc agaggttact tacgaattgc ttgaagaatt agggaagggc
     8521 gctggagaag atgtttcaag agaagttaaa gaacagtgtg taataataac taaatatgaa
     8581 ggttatatag agaaacaatt gaaacaaata gaccaattta aaaaacttga gaataaaaag
     8641 ctggatgaga aaataaatta ttcatctata gaaggtctta gattagaggc tagacagaaa
     8701 ttagatgata taaaacctat ttcaataggg caagcatctc gtatatcggg tgtttctcca
     8761 gctgatatat cagttttact tatataccta gaacaagtca gaagaactag aggtggcaaa
     8821 ggtgaataat atagaacttc tgaaaaacgg gattgaaggt tttaatatag atacgaatga
     8881 cagtatgtta gaaaaattta aaatctatag agagatatta gttgaatgga ataaaaagat
     8941 gaatcttact ggaatagaag atgaaaaaga agtgtatata aaacattttt tagactctat
     9001 ttcagctgta aagaatggat atataaaaaa tgggatgtct ataatagatg ttggtacagg
     9061 tgctggtttt cctggaatac cattgaaaat atgtttagat agcttagaac ttactttgtt
     9121 agattcgtta aataaaagaa taaacttttt agaggaagtt agtagagtac tagagttaga
     9181 taatattaca tttatacatg gaagagctga agattttggt aaaaatgaag actatagaga
     9241 aaaatatgat gttgctacgg caagagctgt tgctggatta ccgattctta tggaattttg
     9301 tgtaccattt gtaaaagttg gagggtattt tatttgctta aaaggtccga atgctaattt
     9361 agagctagaa gaatcacaaa aggctattga tgttttagga ttggagtata ttgaaaaaat
     9421 agatgtagag ttaccagagg tagatttaaa tcataatata ttagttttta aaaaggtgat
     9481 ggaaacacca gttaagtacc ctagaaaagc tggtagacca gctaaaaatc ctattaagta
     9541 atgaaaaaag taatgaaaaa attattattg gtaagcttaa ttatttctta ggaaataatt
     9601 aagcttaccg taaaaaaaca aaaataaagg aattattaaa gtagatgcag aaataaattt
     9661 gtatgtacat atagagaaca aaaataattt atatctaaaa taagagaggt atgttatgga
     9721 agataaaaga gttatggaga tacctataga agatgtagtt ccaaatccat atcaaccaag
     9781 aaaaatattt tcacaagttt cattggagga attgagcaat tctataaagg tatatggtat
     9841 aattcaacct ataacagtta gagcaaaaga tggcaaatat gaactaattg caggagaaag
     9901 aagattaaga gcagctaaat tagcagagct taaaaccata ccagcaatta taaataatat
     9961 gaatgatgaa tcttctgctg ttctagcgtt attagaaaat ttgcaacgag aagacttaaa
    10021 tttcattgaa gaagcaattg gttatgaaaa cttaataaaa gaacatgctt ttactcaaca
    10081 gcaattagca gaaaaattgg gcaaaaatca atctactgta gcaaataagc ttagaatatt
    10141 aaagcttcca aacgatataa aaatgaagct aatagagaat aatttaacag aaagacatgc
    10201 tagagcattt cttaaactac caagtgaaga tttaatgcaa agtgtgttag acaaagtcat
    10261 aaaaaatgaa ttgactgtta aaaaaacaga aaaattgata caagatgtat tagaagaaac
    10321 taaagttcaa gaagagcctg ataaaaaaca gaatataaag ggtgctatga gtataagaat
    10381 ttacataaat actataaagc aggcctttga tgctatatca aatacaggaa ttgatgctaa
    10441 gtataatgaa atagataagg gagattatat ggaagttgtt gtgaaaatcc ctaaaaaata
    10501 gagattactg cttaccagta atcttttttt gtaatataag ccttgtaata taaattgatt
    10561 tatagacatt atttatactt aaaaaacacc tattcaacat ttaaaattaa tttaattctt
    10621 aaagggggga acatcatggg caaagttata gctgtattca atcaaaaggg tggagtcggg
    10681 aaaactacaa caaatgtaaa tttaagtgct agcttaggaa ctttgggaaa gaaaatatta
    10741 gtattagatt tagacccaca aggaaataca actagtggat atggtattaa taaaaacgaa
    10801 gtagaaaata caatctatga aattatgtta gatgggttac atattaaaga agcaataata
    10861 agtactgaat ttgagaatgt agatgtagtt ccatcagcta ctgaactatc aggtgcagag
    10921 atagagctta catctaaaac taatagagag tatattttaa aaaattccat aaaagcggta
    10981 atagatgaat atgattatat atttttagat tgtccaccct cactggggat gcttacaata
    11041 aattgtttaa ctgctgtaga tagtgtttta attccaatac aatgtgaata ttatgcttta
    11101 gaaggtgtta gtcaattaat ggagactata aaattagtaa aatcaagatt aaatgctgat
    11161 atagaaattc aaggtgttgt tttaagtatg tttgatggaa gagctaattt atcaatacaa
    11221 gtagttgaag aagttaagaa atactttaaa ggtagtgtat atacaacttt aattccaaga
    11281 aatgtaagac ttgcagaagc tccaagtcat ggaaaacctg taatttatta tgataaaaga
    11341 tgtagagggt ctgtagctta tttggagcta gcagaagaat ttatagattt agaagaggag
    11401 gaatggtagt atggagaata agtctaaaag aagtaataga ttaggtagag gtcttagtgc
    11461 attgatacca gaaattaaag gagaaacttc agaaaaagaa atagtcaaca tagatataga
    11521 caaaatatat ccaaacgaag ttcaacctag aaagcaattt gatgaagaaa agataaaagt
    11581 attgtcggac tctataaaaa attatggtgt tttacagcca atagtggtta aaatggatga
    11641 aaataataaa tatatgataa tagctggaga aagaagattt agagcatcaa aacttgcaaa
    11701 taaaaatcaa atacctgcta taattaaaga tatagatatg aaagatataa tggagatagc
    11761 cttaattgaa aacttacaga gggaagattt aaattcaata gaagaggctc tagcatataa
    11821 gagtttaata gagcattaca atgtaactca ggaagaaata tcagaagctg ttgggaagag
    11881 tagacctcat ataactaata cactaagact tttaaatctt ggacaagatg ttatagaaat
    11941 gatagacagt ggtagaatta cagcaggcca tggtaaggct ttattgagaa ttgcagataa
    12001 agatttgcaa cttcaaatag cgaaaaagat agaagaagaa gaactttctg taagagaagt
    12061 cgaaaatata gctaaaaaaa tatctgaaaa taaacaagaa gaacctaaaa aatctaaacc
    12121 taaggatgta tttattttag atgttgaaga taaacttaga aatatatttg gaacaaaggt
    12181 gaatatttct aaaggaaaga agaaaggtaa aatagagata gaatactata atgatgatga
    12241 tttaaatagt attgtatcaa tgcttcttga atagaacaaa aatataaaac agagaggaag
    12301 attgttatga tttatttaga taatgctgca actacatatc caaaaccaga aagagtgtat
    12361 aatgctgttt tagattgtat gaaaaattat tgtgcaaatc caggtagagc aggccataaa
    12421 cttgctatga gagctgctag ggagatatat gatacaagag agaatatagc taagttgttc
    12481 aatgtaagta atcctatgaa tatagtattt acatctaatg caacagattc tttaaattta
    12541 gcgataaaag gtgtattaca agaaggagac catgtaataa caacgtctat ggagcataat
    12601 tcagtaataa gacctataaa agcattggaa aaaaggggta ttgaaaatac tgtagtaaaa
    12661 tgcgattatg agggattttt agattatgaa gatttagaaa aatcaataaa gagtaatact
    12721 aaactgattg taacaacaca cgcatctaat gtatgtggaa ctttaataga tataaaaaaa
    12781 gttggagaaa tagctaaaaa acataatata ctatttttag tagatgcatc acaaacagca
    12841 ggtgtttatg atatagacgt aaatgaatgt aatatagata tgttggcaat gccaggtcat
    12901 aaatgcttat ttgggcctca aggaacaggt atattatatg taagagaagg gttgaactta
    12961 aatatattaa aagaaggtgg aacaggtagt aaatcagaag aaatagttca accagagtta
    13021 ttcccagata agtatgaatc aggaactcat aatactccag gtatagcagg tcttaaccaa
    13081 ggtatactat ttatatttga aagaggaatc aacaatataa gacaacatga agaagaatta
    13141 tgtcaatata tgatagacaa actagaggaa gtatcagata taaagatata tggaccaaag
    13201 gatagtaaaa aaagagcatc agtaatcgct ctaaatatag gagatatgga ttctggagag
    13261 gttactttct tattagatag tgattataat atagcaacta gatcaggaat acattgttca
    13321 ccactagcac ataccacttt aggtacttta aaacaaggtg cagtaaggtt cagtatagga
    13381 tatttcaaca caaaagatga gatagataaa gcagtggaag ctttgaaaaa aatttcaaaa
    13441 aataaatagc atgagattta ataatataga aataaaaaaa ggggatattt caaaataaaa
    13501 atttgaatca tctccttttt tgtacaaaaa agaaaagggg ctcctaagaa cctctaatct
    13561 atttgataat tgtttcaaga aattacttta agtaatctcc ttcttcaaga actatatgac
    13621 cactttcgta attagaacca tcaacagtaa tttttatttg ttcaacattc atatttttta
    13681 tgtaagtacg agcaactgaa tcaagcatta aagtttctgc atcactacct aagtttgact
    13741 ttgtaaagtc agagctaaca tctaatatac cagtttttac accatcaatg tcttttgtag
    13801 taaatgaatt taattttgca gattctggaa caactttaag tttttgaagt tcttcaaata
    13861 aagtgttctc atcaatttta tttaaatcaa ctttattttc aattaaatct gtcttatcta
    13921 catcaaaaga gtaaatagta gctgattgag ttttagtttc ttcttttgga gtaggtttag
    13981 ctttgttatt aggaacctct ttttcagtat tgttttcttc ttttggttgt tcaacatttg
    14041 tattgctctt agtttctttt ttagatgtat cacttccatt ttgagcatta gtacaaccaa
    14101 ctgcgaatat agatatagat aatatactta acactaacat tattttctta tttttcataa
    14161 aaatttctcc tctcttaaaa actattctta tatataatta tagtcgttat gagcaaatat
    14221 atgaatacaa aatagtaaca aatttaaaaa atgatttatt taaattaata aaactcaaaa
    14281 taaactaaat tttagtcatt tttatgggaa atcatttaat gtacagtgaa aatattgtaa
    14341 aataaaagta gtgtttttaa aataaatata agttataatt atattataaa aaaatgtctt
    14401 caaaaggtgg gaatagtatg gaaattttga gcttaggaga aaagattaaa aaattaagaa
    14461 aagaaaaaaa catgacatta aaagaattag ctggagatag gataacagca gcacaaataa
    14521 gccatataga gagggataaa tctcatacta gttatgaact gttggaatat ctaggcgata
    14581 agctagatgt aagtgtagaa tacttattag agaccaagga aatgcaatca aaaaagataa
    14641 ctgataattt aatactacag agcgaaattt atataaaaga aaatgaatta aacaaggctg
    14701 aggatcaaat aaaagaagtt ttagatatat gtaagagata cgatttaatg gagaactatg
    14761 gcagatgtaa ttttttaatg gctaatatta atttgaaaaa atctaattat aatgatgcta
    14821 tagaaaactt tgaaaaggca ttgtacttct ttataaaaaa tagtgatagt gaaaatatac
    14881 ttaagtgtta tttaaacata gggaaaatat atatgcagga agacttttat aaaggtgcta
    14941 taagccactt taattttgca gaggaaattt tgtcagagaa taaatttgag gatgttaaca
    15001 tacacaagga actatatagt aagatggcat attgttacat aaaactagat aatccaaata
    15061 tgtcattgaa gtacatagaa aagataaatg aaatagataa tagaaatgat agaaaagaag
    15121 atgtcgatat tttggtgtta aaagcaaata atatgcttga gattggaaaa tatgaagaat
    15181 caagagatta ctttaaaaaa gcactaaaaa tacttgagag tgaggataat aaaactggat
    15241 tagcaaatgt gtatttaaca atttgtgatg tttacagaaa aataggtgaa acagatagag
    15301 tacttgaata ttcacaaaag gtatatgaca taaagaaaaa tgatgaagat gaatacatga
    15361 tgtcaggatt atttaagata atagaagcat atatagataa agaagattat gatttagcta
    15421 gaaaatattg taagatagcc ttagcttcat caataaaaaa taaaaataaa ttcaatgaat
    15481 ataaagcatt gagattctat gctaatatgt ataaaaatca gaatgaagta actcttgcaa
    15541 ttgaatatct gattaaatgc ataaaaatag tatcagattt aggcaacaat agaatattgg
    15601 ccaatttata tatagactta ggtcaattat attcaaatat atcaaaagaa aaagagttag
    15661 aatattatca aaaaggtgta tttatgtaca aaaatttaga aataatgtag ggtaaataaa
    15721 cctataaggg tatagaaaca atctataaca agcaagataa tatctatgat aaaatataaa
    15781 ttgtatgaat atttttaagg agatgaatca gacaatgagt ataaaaatag atgcgagagg
    15841 gcttgcttgt cctaaaccag ttataaatac aaaaagagaa ttggataacc tagaagaagg
    15901 tgttgtagtt acaatagttg ataatgaaac agctaaggag aatatattaa aacttgctaa
    15961 atctttaagt tgtgaagcaa atattgttga cgaaaaagag aattttatat ctatagaaat
    16021 tagaaaaggt aaaaatatac tagataaaca aaatgttgta gataataaag aaagtgaatt
    16081 agacaataca tgtatattta tatcttcaga taagatggga cttggaaatg acgaattagg
    16141 aaaagtgtta ataaaaggat ttatatatac attaacagaa tctaaacctt atcctaagta
    16201 tgtactttta gttaatggag gagtaaaatt aagtgctgaa aatgaagaaa ccatagaaaa
    16261 tctaaaaata cttgagaata tgggtgtaga agttttatct tgtggtactt gtttagatta
    16321 ttataattta aaagacaaat tacaagtagg tacagttaca aatatgtata ctatagttga
    16381 gacattaaag aatgcatcaa ataccatttc tatataatgt atgtttgttt aataatataa
    16441 aactaataat aaagattaac tcaaatagat agtcagaaag gattaagtaa tgaatcaaat
    16501 gtatatagtg tcatttaatt cgacacatca tgcgataaga tcagaaaagt tatttggtga
    16561 aaatagtcta aaagtaatgg ctcttccaac accaagagaa ataacagcaa gttgtggaat
    16621 atcaattaaa ttttcttttg aagatatgga aaagataaaa actattttgg ttgaaaataa
    16681 tgtagatata aaaggaattt attgtataag taaattagaa aatggttcaa aagaagtaga
    16741 gaagttagat taggaggcta attatttatg ccaatggatt taaaaatagg tgatattgtt
    16801 gaattaaaaa agcaacatgc atgtgggtgt aaagagtttg aaatcataag aacgggaatg
    16861 gatataaaga taaaatgtac taaatgttca aggctaataa tgcttgatag agagacctta
    16921 gaaaagagag taaaaaagct aataaaaaag tgataaaaat ttaacactta aaaaatgtca
    16981 aataccgtat attaatatcc aaaattttgg tataataaga attgaagaaa acattttcat
    17041 gggggtgctt taattatggc agttaaatat gcaaaaagaa tgcaaggatt acaaggatca
    17101 gaaatacgtg agcttttaaa acttactcaa caaccacaaa taatatcttt tgctggtgga
    17161 atgccggctc cagagttatt tccagttgag gaaatgaaaa aagtatcagt tgcagtgctt
    17221 gaagaaaatg gaagatcagc tatgcaatat actacaacag aaggatatga accattaaga
    17281 gaaaaaatag cagctagaat gaatgataaa aacaaaacta atgtaaacaa agatgatata
    17341 ttagttacaa gtggttctca acaaggtcta gattttgcag gaaaagtatt tattgatgag
    17401 ggtgatgtaa ttttatgtga aagtccatct tacataggag ctataaacgc atttaaatct
    17461 tatcaaccta aattcataga tgttccaaca gattctgatg gaatgataat ggaagaatta
    17521 gaaaagatac tagaaacaac tgatagaata aaaatgattt acgtaatacc tgacttccaa
    17581 aatccaacag gaagaacttg gccacttgaa agacgtaaaa aattcatgga aatagtaaat
    17641 aaatttgaaa taccagtaat agaagataat ccatatggag atttaagatt tgaaggagag
    17701 actttaccat ctttaaaatc aatggataca aaaggattag taatattctt aggaactttc
    17761 tctaaaatat tctgcccagg atacagatta ggatggactt gtgcttctcc agaaatttta
    17821 tctaaattta actttgcaaa acaaggtgca gacttacaag catcaactat atctcaaatg
    17881 gaagtaagca aatttatgga tatgtatgac ctagatgcac atgttgacaa aataaaagca
    17941 gtttatgtta aacgtagaga tgtaatgctt aagactatgg aagaagagtt cccagaagga
    18001 ttagtattta ctcatccaga aggtggatta tttacatggg tagagcttcc aagtaattta
    18061 aatgctaaag aattaatgcc aaaatgttta gagaagaatg ttgcttatgt tgctggagga
    18121 ggattcttcc ctaatggtgg tagagaaaat actttcagac ttaactattc aaatatgcca
    18181 gaagaaaaaa taatagaagg tataaaaaat atagcagctg tattaaaaga agctatgggt
    18241 gtagaagctt aattaaaaat aataacttat atataataca agggatggtt gttgccatcc
    18301 cttgtttgtt tgaaaagtat ataattcacc ttatactaaa ctttgtccaa aatttaaatt
    18361 gacagaaata agaataaatg ataaaatgtg taggtatgag attttagtct catttctctg
    18421 ctctaagagt gtagagccgt taagtccaaa gggaggtgaa ttatagtgag aaattatgaa
    18481 ttagtttatg tggtaaagcc aaattctgat gaagaagtaa gagaggctat actaaacaaa
    18541 gttaaggaag ttgttgcaac tgacggagaa atagttaaag ttgatacttg gggaactaaa
    18601 aaattagctt atccaatagc taagtttaca gaaggtttct atgtattagt taacttcaaa
    18661 tcagcagtag acgttcctaa agagatagac agaaacttaa agataaatga aaacgtaata
    18721 agacacatga tagttgttgc ttaataactg gttttattat aaaaaatagg tggtgttttt
    18781 atgaatcaag ttgtattagt gggaaggttg actagagacc ctgaattaag atacatccca
    18841 ggtacaggaa caccagtggc atcctttaca atagccattg atagagacta tgtaaaaaaa
    18901 gatggatcta aagaaactga ttttatacct gttgaagtaa tggggaaatc tgctgaattt
    18961 tgtgctaact acataactaa aggtagatta gtagcacttc aaggatctat aagagtagac
    19021 aattatcaga cacaatcagg tgaaaagaga acttttacaa aagtaagtac tagatcagta
    19081 caagctttgg atagtaaaaa taaatcggaa aattcatata gagagagtgc tccagctttt
    19141 gagccaagct ttgaacctca aggtttagac ccacaaggtt ttcaagctat agatgatgac
    19201 gatataccat tttaaaaagg agggaaaaaa gtcatgatga ataagaaaag acgtaagaaa
    19261 aaaagagttt gtcaattttg tgctgacaaa aacgctaaaa tagattacaa aagtactcaa
    19321 agattacaaa agtatataac tgaaagagga aagatattac caagaagaat atctggaact
    19381 tgtgctaagc atcaaagaga attaacagtt gctataaaaa gagcgagaaa tatagcactt
    19441 ttaccatata cattagacta aaaaaatgag ccttgtgctc attttttatt atatgtattt
    19501 atatatctaa atgaggcgag ctataaagct cgtttttttg tttatatact taaatatatt
    19561 attatttatg atataatatt atatattaag gagggttgta aacgtgaaaa tagataaagg
    19621 ttctgagata acgagaaaca taaagataat agaatggatg aaaacagaaa tattaatgag
    19681 tgtaagtgat ttgtttaatt tattgtttaa aggggtaaaa ccattagatg aagcaattca
    19741 agatacttta gcaaatataa taatgataac atatctctta gcaaagagat taggaattag
    19801 ttttagagat gtggattata aagtcaagga aaaaataaaa attggtatag atgaggacca
    19861 tagcgttgaa agatggtatg gagatttatc taatttaaaa aaacatatgg ataataggga
    19921 gtgatattaa attgaataat aaaattagac tatctaaggc aatgtctata atagttttag
    19981 ctataataat cgctttagta atagcctata ttccaatgct aggaatattt agtatgttag
    20041 cagctgttcc atatgtaatt ataggtgcta ttactgatag aaagtattct cttatatcta
    20101 ttttggttac attttgtgta ctgattctat ttgcagacat ttcttatgca ttaaatatat
    20161 gtataatgta ttctttacca ggtatagtaa taggaaagat gctaaagaga agctttgaac
    20221 aagaggacag taataaattc gaaccgatat atggtggaac cataatattt gtactttcaa
    20281 tgcttgtata tttttttgta ttaaagacat ttttaaatgt gaatctatta gatgaagttt
    20341 ctaagatgat atcagaaatt gtaaatattc aaaaaaatag tttttctgca actgaactta
    20401 aagtgtttga taaaatgaag ccagatgaga ttgtaagtta ttttacaaat atgctaccaa
    20461 tgatgttatt tttacaaggg ttgttatcag cgtttgtcac atattatttg agtgtatttt
    20521 ttataaaaag gattacaaag atgaatataa gatttcctaa atttgctgat ttttatttac
    20581 caggaaatgc tatactcaca actttcttac tttatttatt agtcttattt attcaaatta
    20641 ttggttcaaa attatataca gaattaataa tgactaattt acagctagtt tttaatttaa
    20701 tgtttgtaat tcaaggtata gcagtttgca tttatttctt aaagaaatgg ataagacagg
    20761 gaccaaataa aattatgttt ttttcaggga taattttatg tttatttgga tttatgggaa
    20821 tatcttttgt tggtatggta gatagtataa ttgactttag aaaggtaaga agctataaat
    20881 ccacttagga ggatagaaat gagtaacaaa caaaccttta aattaaatat gccagaaata
    20941 aatttatata taattgttat tggtatttct agtataattt tgctttatta caacttgtat
    21001 gtagggtgtt tatttttctg tatttttgta tatatggttt ttcacaattg gagaactact
    21061 aatattagac gtcatgaatg gacagagtat attcaaaatt tatctttaga tatagatgaa
    21121 acaactaaaa aagccataat aaatcttcca ataccattat gtattttaga atttgatgga
    21181 aatatatcct ggtataatgg aaagttttat gatatgattg gtcaaaaaga tttacttgat
    21241 aaaaatatag aggatatagt taaaaatctt aatttaagaa aagtattaaa tgaaaataaa
    21301 gagatgtaca cagaaataaa ctataaagaa aaagaatata caataattta taatgttata
    21361 aaaaatgatc aagaaaaaaa tcctaaatat ttgatgatat tatactggat agacaagact
    21421 gaatatttaa aagtaaaaca aaattatgat gatgaaaaaa atgcaatgat gctaatacaa
    21481 gtagatggat atgatgaagt attaaaaagt gcagcagaag ataagagagc tttaattaat
    21541 gttgaagtag agaaaatact ttctgcttta gaattaaatt caaatggtgc attgagaaga
    21601 acttcaaaag acaaattctt cttggtaatg cataaaaaag agctaaaaaa attggaagct
    21661 gagaagtttt ctattttaga tacaataaga catattgatt atggaaataa tcttcctgtt
    21721 actataagta taggaattgg tatagatgga gatacattaa atgagaacct aaaacttgct
    21781 acgggtgctc tcgatttagc acttggtaga ggtggtgatc aggctgttgt aaaaacaaaa
    21841 gataaatttg tgttttatgg aggaaaatct aaagctgtag aaaagaaaac caaagtaaaa
    21901 tccagattaa taggtcatgc tttaagagag gttatacaac aaagtgacca agtttatata
    21961 atggggcata aatatcctga tatggatgct atgggagcag cagttggtgt ttatgatata
    22021 tgtaaatcct gtaacaaaac tgcaaacata gttttacaat ctgtaaatga atctatagaa
    22081 atatttataa ataaaataaa tgaaaataat tactataaaa agctgtttat tggaaaagag
    22141 gaagctattg acaactgtac taaaaataca ttagttgtag tagttgatac tcatagacca
    22201 aattatactg aatgtgaaga actgttaaag ctatcagaga aggtagtagt tatagaccat
    22261 catagaagag gtgtggagtt tataaatgat gcagtgcttt tattccatga gatatatgta
    22321 tcctcaacgt gtgaaatggt tacagagtta gttcagtata tggatgaaga tgtaacaata
    22381 aataaactta cagctgaagg gcttttagca ggtataagtc tagatactaa aaactttgca
    22441 ttcaaaacag gtgttaggac ttttgaagct gcttcttacc ttagaaaagt tggagcagat
    22501 actatagagg taaagaaatt cttcaattct gatgtaaaag atttcataat aaaagctgaa
    22561 ataattcaaa gtactaaaat aattaataat agaatatgtt tagcatattc aagtactgag
    22621 atagatagta taaatgttat aatagctcaa actgctgatg agttattaaa tatcaaagaa
    22681 gtagaagctt cctttgtatt aggtgaaaaa gatgatacta tatttataag tgcgaggtct
    22741 ttagggcaaa taaatgtaca tgtacttatg gaaaaattag gaggcggggg acacatagat
    22801 atagctggag cacagttaaa aaatgtttct ctaaaagaag cttataagat ggtaaataaa
    22861 ataatagagg agtatttgga ggaggaagaa tagatgaaag ttatattatt aaaagacgta
    22921 aaaggaactg gtaaaaaggg agaaatgaaa gaagtaagtg atggatatgc aagaaatttc
    22981 ttatttccta aaaagatggc tgtgcaagca gatagtgtag caataaaaga attaaatgaa
    23041 aagaataaat ctaaagaaat aaaagcacaa aaagagtatg aagaagcagt tttattaggt
    23101 aagcaaatgg aagaaattaa tatagaaata tattcaaaag caggagaagg tggaagatta
    23161 tttggttcaa taacttctaa agaaattgct gagcaattaa agaaacaaaa agacatagac
    23221 gtagataaaa gaaaaatatt attagatgaa ccaataagat ctttaggttc aactttcgta
    23281 gaaataaaaa tacatcaaaa agtaactact aaaataagag tggatgtaaa agaaaaacag
    23341 taataactag aggtgattaa gatggaagat atgacgagaa ttcctcctca tagtgtagaa
    23401 tcagaacaat caatattggg ttctatactt ctagataaag atgctataat aacggttaca
    23461 gaaactataa agcctgatga tttctataaa gaggctcaca aaataatata tgaatgtatg
    23521 ataactttaa gtaataaagg tgagcctata gacttgataa cattaacaga agaactaaga
    23581 aaacaagggc atcttaatga tattggagga atcagttata ttacaagtct ttctactata
    23641 gtaccgacga cttcaaatgt aaaatactat gctgatatag taaaagaaaa gtctgtactt
    23701 agaaagctta taaaagcttc taatgaaata ataaatctag gatatagtgg agcaactaag
    23761 attgaagatg ttttagaaca agctgaaaag agtatatttg atatatcaca ggagaaaact
    23821 agtgatgatt ttaaatctat aaacttagtt ttgatggatg cctatgatat gatagaaaaa
    23881 ttatatacta acaagagtga tgtcactggt ataactactg gttttaaaga tttaaacaaa
    23941 aaaataaatg gtcttcaaag gactgattta atattaattg cagctagacc agcaatgggt
    24001 aaaactgcct tttctttaaa cttggttcaa aatgcagcct taaaaggtga tgcctctgta
    24061 gctgtattta gcttagagat gtctaaggaa caattagtac aacgtatgtt atcttcacaa
    24121 tctagtgtag aattaaaaaa aataaagact ggaacactta atgataatga ttggcctaga
    24181 attatagatg ctatggcagt attatcagat gccaaaatac acatagatga tactcctggt
    24241 ataaaaatat cagaactaag gtcaaagtgt agaaagttaa aaatagaaaa aggactagat
    24301 ttagtgctta ttgattatct tcaacttatg gaaggtgaag gtaataatga aagcagacaa
    24361 caagagatat ctaagatatc aaggtcacta aaaatattag caaaagaatt aaactgtcct
    24421 gtagttgcac tatctcagtt atcacgtgca ccagaacaaa gagcagacca tagacctatg
    24481 ttatctgatt taagagagtc tggagcaata gaacaggatg cagatatagt tatgttctta
    24541 tacagggacg aatattatca tgctgattct gagagtaaaa atataggaga agtaataata
    24601 gcaaaaaata gacacggtga aactggttct gtggaacttg tttggcttgg tgaagttcaa
    24661 agatttgggg ataagttgag agacctataa tctaacgaat atccacagaa cgtcctttct
    24721 ttctacagga aaaatgtgga tacatgtcac taaatatgtc caaacattga aatttagcta
    24781 aaaaactatc cacactatcc acagatagcc tgttgataag tgtgaataag tagataaagc
    24841 aagcttttgg cttgctttat ctgtgtataa atattaatta ttaggaggaa taatatggaa
    24901 aaaataagct taaaatatat atatcccaat ataattaaag tacttgatga aataaactta
    24961 tttagagtaa ttgacaataa tttaagagag tctattgttg tttatgctaa taatgtggat
    25021 aatcagtatc atataaacat gactaataca aactttggaa acataattaa catatgtaaa
    25081 ttagaaaaac ttttagatgt ggataaattt atggaaaaag ttataaaata tgaaaaagaa
    25141 ataatagaga aagaagagtt ttcaaaaata gaagaatata tgttaaatat tggagaatac
    25201 taaaaaagtt gaaaaaaata cgaatgtttg atagaataag taagtaaagt gttcgagagt
    25261 atataaaata tagaggtgaa ttacatgaaa acagttgcaa ttgttggttc tcaatgggga
    25321 gatgaaggta aaggtaaagt tatagattat cttgctactc aagcagatgt agtagtaaga
    25381 gggcaaggtg gaaataatgc aggacataca ttagttgtag aaggtaaaaa atacgcatta
    25441 catctaattc catctggagt tttaaatcca aatacagtga atataatcgg aaatggaata
    25501 gtgtttgacc ctaaaggatt tttagaagaa ttggaaatgt ttaaaacgga taatataagt
    25561 acagaaaata taaaaataag tgatagagca catgtcatat tcccatatca taaagaacta
    25621 gatgcattgt cagaagaagc cagaggcgat ttaaagatag gtacaacaaa aaaaggtatt
    25681 ggaccttgtt atatggataa aacagagaga tctggtatta gaatatgtga cttaatggat
    25741 aaagataaat ttgctataaa attaaaagct caaatagatg ctaaaaataa aatagttaaa
    25801 aatatatatg gaaaagaaga attatttgat tttgaaacta tatataacga atatctagga
    25861 tatgcagaac aaataagaaa atatgtggca gatacgtctg ttattgtata tgatgcagta
    25921 agagcaggta aaaaagtatt gtttgaagga gcacaaggga ctttattaga tttagattta
    25981 ggaacttatc catttgttac atcttctcat ccaacatctg gtggatttgc aattggtgca
    26041 ggtataggac caaatatgat aaaagacgtt gttggtatag taaaagcata tactacaaga
    26101 gttggagaag gaccgtttgt tacagagcaa ataaatgaaa ctggagataa gataagagaa
    26161 caaggacatg aatttggggt aactactggt agaccaagaa gatgtgggtg gtttgatgca
    26221 gttatagtta agtatgcagc tagagttaat ggattgacta gtatttcatt tatgttactt
    26281 gatgtattaa ctggatttga taagataaaa gtttgtacgt cttataaaat gggcgataag
    26341 ataataacgg atttcccagc ttcattagat gatttagcaa aatgtgaacc tgtatatgaa
    26401 gaattagatg gatggaatga agatataact caaatagata actttgatga ccttccagaa
    26461 aatgcaaaga aatatgtagc aaaaatagaa gaactagttg gagtaagtgt agatatggtt
    26521 tctgttggtc caaatagagc tcaaactata ataagaagaa atatatttgc ttaatatttt
    26581 atcaattaaa taatttaaaa tagttgaaga agagtatctc aagataatgt ttatagttat
    26641 tagagatgct cttttatatt tgacttacat tgatttttta ttaagttaaa gctacttata
    26701 catattctag ctgaaattaa agttgaattt atgtagatac atataggttt tttaaaaaga
    26761 attttgttga aaataaatgg ctaaagatga ttttaattat attctaaaaa aagtaaaaaa
    26821 aactattgac taaaaattgt atagacgcta taatataact tgtgagtgaa agaaacgacg
    26881 aaaaaagtcg aaaaaatgtg gtctattagc tcagtcggta gagcacctga cttttaatca
    26941 gggtgtcccg cgttcgagtc gcggatagat caccaaaata tggctcgttg gtcaagaggt
    27001 taagacaccg ccctttcacg gcggtaacag gggttcaatt cccctacgag tcaccagtat
    27061 gggactatag ctcagctggg agagcacctg ccttacaagc agggggtcac aggttcgagc
    27121 cctgttagtc ccaccaaatt ttgaattaga gataaatact agattatatg ctggtgtggc
    27181 tcaacggtag agcagctgac ttgtaatcag caggttgtag gttcgattcc tatcaccagc
    27241 tccacaaagt acataaattc aaattaatat aattttctat gttacaggat acttttaagt
    27301 atcttttttt aatttcattt ttattgtatt aaaaatgaaa ttaaacatac aacaaaattt
    27361 tgaattactt gcaatgtgat agttttaaat ataaaattta gaatcattct agcatagtat
    27421 tatgtaatat agcaaattta atttatagta tcaataaata tagactacta agaatgtaaa
    27481 tgatttgtta gattaagata cattagatat gtaaatagaa aaataaaaaa tatgttttaa
    27541 tttatgtggc ataagtttta aacagagaag caagtttgct cttacctctc tatttaaaaa
    27601 taaaaaaatg ttttttaagg gctattttat ttaaatcttt cccatctcaa atcatctcca
    27661 gtaaattcta aaatcctaaa cttatctaag attctagaga gagttctttg agtgtaagta
    27721 tttcctattt gagatggtgt taagttggtt gaaataatga tttttttacc agcaacaagt
    27781 cttgtattta caatattgaa gatttcccca ctagtaaaag aattattaag ttcagttcca
    27841 aggtcatcta ttattaataa atcacaatca aacaagtttt tataattatc ttcacttatt
    27901 tggttatttg tgtctcttct aaatttataa tcttcaagaa tatctaaaat tctaaaagaa
    27961 gtttgataga ttacaacatt tcctttatct aacaattcct ttgcaataca attgcacata
    28021 taggtctttc ctagtccagt agaaccataa aacagtagat tttcactatt atccttctta
    28081 aaatcatgaa caaaattttc gcagatactt aaattattta gcatattttc tctaggagat
    28141 atttccccat cagagccttt cttaggagaa aatatattca agttaaaatt cgaaaaattt
    28201 tcctgactta aaatcctatc taaattagac attttataag cttcatttac aatttcttgt
    28261 ttgaggcaag aacatttttc tccattaggt aaaaatcctt tatctttaca agaaatacat
    28321 tgaaacttta tttccaagta atcaagaggt atgttacttt cagataaaag tttttctttc
    28381 tttactttta aagactctat attctcttta gtcttcttta caatttcatc tttagatttg
    28441 ggatttaaaa gtactatctt agctaaactg agacctattt tagaaatttc atcatctata
    28501 gattttatct cagggattct attatatacc tcattttttc tatgttctaa taagagttca
    28561 ttatcatctc ttctcttagc atatttagca agtatttttc ttattttatc ctcattcatt
    28621 taatcaccta cttaaacttt tctttttgac tttttttaat aatctcatcg agctcatcag
    28681 aggtatactg agtgaatgtt tcgttgaaat tatgaaactt agtttttttg taagtattgt
    28741 tgttttgagt agtattaatc tgcttcttat taatattctc cttaactata cgttcttcct
    28801 ctttttgctt taaatcattt aaatttttta tatttttttc attccaattt ttaattatac
    28861 catttatata agatatactt gggtttgaaa tattcttaga cttagaacat gcatttatta
    28921 tcaaatctat atccatatta aatttatcaa accaggtgtc cataagttct ttttctgatt
    28981 ttgatggttg tcttgaaaat cccaattcat tgaaaatcgt tttatataaa atatatcgtt
    29041 cacttcttac caaaaagctt tcttcaacat cttttggcgt atataaattt gaatcatacc
    29101 aatttctaat aatgccttca atgtatttaa ctggctttga tgtaccagtt ttatctttga
    29161 cgtattcata tgcgcaaagt atcatatcgg ggctcatatt gtatttattc attatatcca
    29221 taatactcat cttttcactt gggtcgagat atcgcccaac tattttattt atagaattaa
    29281 acatttttct aatacttgga ttttcagaag tagatacaac cttatctgta ttagatttta
    29341 tagatgaatt attgcttaat atattctcaa tatagaaatt ttttaaatct aaaaattcta
    29401 ttgaataatt aaaattatca tattctccat tgtcatgtat ttttataatc ttcttttctt
    29461 cccagaattt ccaagcagat aacacatcag acaatggtat atttaggttt ttggctattg
    29521 aattattgtc gaacttagga ttagaagtaa tatcacatgc ttgtctatat cctaaaagat
    29581 aaacttttac gtataacccg tccgccattg gcataaatat atctataaag atattgggaa
    29641 tagtgatttc acctaaatct atttcattgc tttctttaaa aaacatttta tcacctcaga
    29701 ttaaattata tcatttttta tattatattt gtctgtaaac aaataaaagg tcacaagatt
    29761 agcatgtaaa tattttctaa aagtattcat tttgaaaagg aggagatatt ttggttataa
    29821 aattcaacca aaaagtaaga agaacattat cagtagcgat tctatttatg gtattaataa
    29881 taggttgctt tatagaaaag gatgacagtt tagaaacata caaagaaaaa actaattcaa
    29941 aaataaatca gtataatatg gatgttatat ttgatgatga gactaagagg ttaatgtgca
    30001 atcagaatgt tgactacata aataatacaa aatcaaacat agataaaata tatttccata
    30061 tatatccgaa tgctttttcc aagaaagatt ttgctccttt tgaaaaaagt gaaatgacaa
    30121 gggcttatcc taatggattt aacgaaggat atatagatat aaagaatata ttaaataata
    30181 atagtaaaat ggagtataaa attaaaggag acaaaaatga catattagaa atagaattag
    30241 ggaaagagtt aaaaccaaat gaaagaatgt cattagatat aaaatataac gtaaaaatac
    30301 caaattcaat tggaagattt ggatatggag aaaatactat aaatgttact aattggtttc
    30361 ctatagcttg tgtaaatgat gaaaggggat ggaatttaaa gagttatgaa actattggcg
    30421 acccatttta tagtgagact agcgattttc atgtgaaact attgattcca aataaatata
    30481 aaattgctca tacaggaaaa cttattgatg ataaacatga taataagaaa atgctatatg
    30541 aaatagaagc tgaaaacgtt agagattttg catttatact aagtagtaaa tttgatgtaa
    30601 acaaagtaga ttcaaaggga gtagctatca atacatataa tttaaataaa aagctttcta
    30661 aaaaagcaac tgaagtggca aaagattcaa taaatatatt tagtgaatta tttggaaagt
    30721 atccttacaa agaattttct gttgtagcaa gtgacttttt tatagggggg atggaatacc
    30781 ctatgcttgt tatgatagac caaagtttat ataatgaaaa aaatgaattt ttactagaat
    30841 atgttattgc ccatgagaca gcacatcaat ggtggtattc tgctgttgga aatgatgaaa
    30901 taagtgaacc ctggttagat gaagcactta ctgaatattc tacaatagtt tattttgaac
    30961 aaaagtatgg taaagatatg gcaaacaagc ttataaaaac tatggagata cagacaaaaa
    31021 gttatttgag tgaaaatata tttaagccag caaatgagta taaaaattct actgaataca
    31081 gcttgaatgt ttatacaaaa ggcgcaatag catttaatga aataagaaaa gaagttggag
    31141 ataaagtatt ctttgagact ttaaatgaat actataataa atataagcat aaaaatgcta
    31201 atggaagagc ttttgtggaa ctatggaata gtaaaggtgt agatataaat aagattataa
    31261 gtgaatgtaa gtaaatagtg aatgtaagta aattttgtgc agagtattaa attttattta
    31321 atactctgtt tttttggtat aatataattt ataaggcaga aaaagaggtg aaggaatgct
    31381 aaagtattgt tcagtaggga gtggtagtag tgggaattgc cactatatag ggtataaaaa
    31441 tactaatata ctagtagatg caggattaag tggcaagaga ataactacag gtcttaaaga
    31501 cattggtgta gatgcagata aattaaaagg aatatttata acacatgagc atgttgacca
    31561 tataaaagga gcaggtatat tatccagaaa gttcgatata ccagtatttg ctaatataaa
    31621 aacatggtgt tctatgaagg acaaattagg tgacatcaaa gataaaaata tgaaagtatt
    31681 tgaaaatgat aaaacctact cattaggtga tatcatagtg agaccttttt caatagaaca
    31741 tgatgcgtct gatccagttg gatataattt ttatacagag agtgatgaaa aaatgtctat
    31801 agctactgat ataggatgta taactcaaaa tataaaaaaa catctatata aatctaaatt
    31861 agtagtattg gaatcaaatt atgaccctaa tatgcttatg atgggttcat atacatatgc
    31921 tttaaagaaa agagtaatgt caagtactgg tcatttatcc aatgaagatg cagctaattt
    31981 ctgcgttgaa cttattaatg aaggtacaga atctatatta ttagcacatt taagtaaaga
    32041 aaataatttt cctgagttag catatgagac ttcaaaagga gtattagcat caaatgatat
    32101 agtagttgga caagatgtaa aactagatgt actatcacga aatgatgttt ctaatgttta
    32161 tgagatgaag tagaggtgaa taactttgct aaagagaaat tttgaaaatg ttgaggataa
    32221 taaaatttta tactctataa agcagggtat gattttagca atacctgcta ttatgactgg
    32281 ttcaacggct cttgtaattt tgaatcttcc aataaaggct tatcaagaat atttatcagg
    32341 cttgtttaat ggagaggtta caaatattct taactttata aatgattcta ctttaggaat
    32401 tatttcatta ataatattat taacaataag ctatagttat ggaaaaatat atgggagcaa
    32461 gtatacagta cttgttccta tagttgcaat gtgctctttt ttagtatttt cacatggtaa
    32521 tgagcttaat tcttatatag aaatatttaa gacaaagtgg ttatttacat ctataattgt
    32581 ttctatgaca tcttctgtat tgtttgtaaa acttacagaa tcttttgtta caagtgtaaa
    32641 gtttcataca gaaggggctg atgcagactt taatatggta gtttcggcta tagttccttt
    32701 cataatagta gtatttttgt tttctatttt tagagtgata atgataagtc taattggcag
    32761 ttcaaacttt caagatatat tttataattt attttcaaat gtttttaata aaatgggaac
    32821 aaaccttatg agtgctctgt tgtttatttt tttaatgcac tttatgtggt tttttgggat
    32881 tcatggtagt aatgtattag ataccgtcgc aaaaaattta tttgaaaata gtatggcaat
    32941 taacataaat ttagttaata ataatcaatt accaacagaa atttttacta agaccttttt
    33001 tgatacaatg gttttattgg gaggatgtgg ctctttacta tgtttagtaa ttgcaatttt
    33061 tcttagtgaa aaacgtataa atgtaagaaa gctagctaaa attgcttcaa taccagctct
    33121 ttttaatatc aatgagatgc tagttttcgg gataccaatt gtttttaact atattatgtt
    33181 tgtacctttt gtaattactc ctataatctt aaccattaca agttatgtgg ctatgtctac
    33241 aggtattgta ccatgtacag tttcagctgt agaatggacc tcacctatat ttttaagtgg
    33301 atatatgtct acaggttcaa taagaggtag tttgttgcaa gtatttaatt tatgtgttgg
    33361 tgttatgatt tatattccgt ttataaaaat gtcgcaaaat agatatacat atatgtttaa
    33421 gaataatata gaaaatttaa caaacttatt aaaaaggtat gaattaacag gtgaacagcc
    33481 taatcttcta aaccataatg gtaaaatagg ttcaatttca aaaatgttgg catcagactt
    33541 gaagtttgct atgaaaagag gagaaataga acttttttat caaccacaag tcaattataa
    33601 tggaaatgtt gtgggagcag agggattact tagatggaaa cattcaatag gagggttcat
    33661 ttatccacca ctagttataa ttttggctaa agaagaaggg ttcttagatg agcttggaga
    33721 gtatattatt aataaagcat gtattgattt aaagaagagt gaaaagctat tgagaaatcc
    33781 tgttaagttc tctgtaaata tatctccaga ccaacttgat gaccctaagt taccggagca
    33841 aataaaaaat atcattagta gtaatgatat aaatcctaat atgttaggta tagaaataac
    33901 agagcaaatt gcattatcag gttcacaaat aattattgaa cgtatcaatg cgatacataa
    33961 cttgggaata aagttaatta tggatgattt tgggatgggg catagctctc ttatatatct
    34021 tcaaaataat aactttgata tagttaaatt ggatggttca ttagttaaag agatacttac
    34081 aaataaaaga agtagtgaaa ttataatgac tattgtaaat ctttcaaaaa atttagattt
    34141 tgatatagtt gttgagtatg ttgaaaatat agaccaaagg gataagttat atggtttagg
    34201 atgtgaaata tatcaaggat attattatag caaagcaata ccttttgaag agtttgtaga
    34261 atatgcaaat caagaaaaag agatataatt ttataagagc ttgtaaattc tagtaatgca
    34321 tagatttgta ataaaattaa gtttaggaga aatatatgaa tataagtata gtagtagtag
    34381 gtaaaattaa agaaaaatat ttaaaattag gtattgatga gttcaaaaaa aggctttcta
    34441 agtattgtaa gttagaaatt atagagttgg atgatgaaaa agctccagag aatttgagtg
    34501 ttaaggaaat ggaaattata aaagacaaag aaggaaagaa aattttaggg aagataaagc
    34561 ataatagtta tgttatagtc ttggctattg atggtaaggg tttatcctct gaagaacttg
    34621 ctaagactat gagtgattta gcagttagag gtaatagtag tctctgtttt ataataggag
    34681 gttctttagg gctttctgat gaggttttag gaagggctga ttataggtta tccttttcta
    34741 agatgacttt tcctcatcag atgatgagat tgatattgtt agaacagatt tatagggctt
    34801 ataggataaa taatggagaa ccgtatcata aataaatgat acggtttttg ctaatttgac
    34861 acttaataat attaaaataa caaaaaagta ttgaaagtat attgcattta aggtagaatt
    34921 aaataaaata ttataacatt ataatgttta tatagttgtt aataaaaatt tggaggaaaa
    34981 tatatgataa aaataatgtt ggcctgttca gcaggaatgt ctacaagttt attagttact
    35041 aaaatggaaa gtgcagctaa agaaaatggt atagagtcgc aaatttgggc aataccagaa
    35101 tcaactatac aaaatgaaat agaaaaatgt gatgtattat tactaggacc acaagttaga
    35161 tatgtattac ctaaggctca agaaattgct aagccatata atataccagt agaggttata
    35221 aatatgatgc actatggaac agttaatgga gaagctgttt taaatagagc gatagaactt
    35281 aacaacacta agtaatataa attataatgt atttttaata tataaattgg gagaatcaaa
    35341 ggatggatga aaagattata gaaatatctt ttaatataat aggttatgca ggtgaaggta
    35401 aagggttagc ttttgaagca ataaaagagg ctaagaatgg aaatatagaa aaagcaaaag
    35461 aattattaaa agagtctaag gaagttataa ataaagcaca tagatatcaa acagaattaa
    35521 tacaaaacga agctagtgga aacaggactg aaataagtgt catacttata catgcacagg
    35581 accacctaat gaatggtatg aattttcaac agttagctga agaaatcgtt gatttacatt
    35641 tgaaattaca agataaataa aaattaaaga gaataaatta agagagttct taaaagatac
    35701 attatgtgta gcacactaat tgttagatta ttgattcaat atttagtgag taatacatta
    35761 ggattatttt gaggactctt tttatattta gtttaaagtt aataaatatt tattattaat
    35821 aatataagat tatatgttat aatattataa catataattt aagaggtgaa tcatgttaag
    35881 aaatcaacca ttatatatac aagttgtaga tatattaaag caaaaaatat taaataacga
    35941 atataaaatt gatgaaaata ttccaacaga aagagaatta gagcaagaat ttggagtcag
    36001 taaaattact attagaaaag ctattgagat attagaatca gaagggtatg tagagaaaaa
    36061 aagtggaaaa gggacaaaag taataagtaa tagtcttttt aataagctat caaaagcaga
    36121 gtctttttca agtatacttt tatctaaagg tcataagtta tctaaaaaga ctatttcaat
    36181 tgaaaaaata ataaatgata caaattcaca agtatttagt atatttggag aaaaatgtag
    36241 caagattact agaatgtatt acttagatag taagccatat atatttttta ctcattatat
    36301 tccttttgat gaaaatctaa ctgttgaaag ttttaataat gaaagttctt tatatatgta
    36361 cttatatagg agaaatataa atataactag atttgaagat gaatttttta tagaagaatc
    36421 agaactagag gtatcaaagg ctctaaatct agctaaagac atattattag gaagaaaaag
    36481 aaagacctat gatgagaatg ataatatagt tgagctatca tttgcaagat ataatacaga
    36541 agtacaaaac tacgtaatta aatacgaaat ataataagaa tcctgagttt attgaaatga
    36601 taatatgctc aggatttttg tttagttgta aaaacgaaaa atattataac attataatgt
    36661 ttacaaccaa acattataat gttataatat ttttaataaa taagtgatat tatcaattac
    36721 ataaaaatta gtttatgtta tgaaatcgtt attgcattat aaataaagga taatatcaat
    36781 aatacagggg gataactatg gaaaaattcg caagtttttt agagcgcaag attatgcctg
    36841 tagcagcgaa gataagtaat cagagacata tgagagctgt aagagcaggt ataatagcaa
    36901 cattaccact aacaatagtg gggtcatttt tcacaatatt attaaacata cctattgatg
    36961 cttatatgga ttttatagct ccatttaaag caacattaga tataccattt agatatactg
    37021 taggattttt atctctatat gctacttttg ggatagcatc atcacttgct aaaagctacg
    37081 atttagatac aacaggtgtt ggtatgcttg cagttatggc atttttagtt tctacaatag
    37141 ttccaactca agtagttgaa ccagtaggtg gagttataga agctggtaga tggatggata
    37201 tggcaaaact taattcgcca tcattatttg gagctatcgt aacatcatta attacagttg
    37261 aaatatatcg ttttatgaag gaaaagaata ttacaattaa gatgccagca ggagttccag
    37321 tagaggtttc aaattcattt actgcattat taccgacagc agtaatttta atgttattct
    37381 gggtaataag acatatttta ggatttgata taagtgttgc actaagcgct atgttatcac
    37441 ctttaaaagg attcttatca ggaaatagtt taggtggtgg gctactgaca gtattcctaa
    37501 taactttatt ctgggtactt gggatacatg gtccagctat aatggggcca gtaataagac
    37561 ctatatggga catgtctata gcagaaaata taactgcatt cacagaagga gtatcagctc
    37621 atgcaatgcc aaacatattt acagaacaat ttttacagtg gtatgtatgg atagggggag
    37681 caggggctac tcttgcatta gttgtaatgt ttatgttttc taagtcacaa tatctaaaaa
    37741 gtttaggtag attaggattt ttaccagggt tattcaacat aaatgaacct ataatatttg
    37801 gagcaccaat agttatgaat ccaatactag ggataccatt tataattgga cctataataa
    37861 tgacaattct atcatatgtt cttactatta caggggttat acctatgatg gtagcaagat
    37921 taccatttgc aatgccagca ccaatagcag cagtaatgag tactaactgg agtatactag
    37981 caggtatctt agtattaatc aactttgtga tatcatttgc tgtttattat ccattcttca
    38041 aagtttttga aaaacaacaa ttacaaagag aacaagaaga aatgagtgaa aatttataaa
    38101 aatacataga tatagaggtt ttgtactata taaaatatag aaaaagctta taagttatta
    38161 agggtgaagg taggaggaat gacctttgcc tttaatttta tatattttaa ataatgtaat
    38221 ttgaggagga gaaagatgga aagaagatta ggaatatcag tatatccaga gcatagtaac
    38281 atggaagatg ataaggcata tataaaaatg gtttcaaaat acggatttaa aagaatattt
    38341 atgtgcatgc tttcaattac aagacctaag gaagaagtta aaaaacattt taaagagatt
    38401 ataacctatg caagagaatt aggatttgaa gtaatccttg atatagctcc aaacatattt
    38461 gatatattac aaatatctta tgatgattta acattcttta gtgagttaaa tgctagtgga
    38521 ataaggcttg actatggata tgatggaagt aaagaagcta tgatttcttt caatccatat
    38581 gatttaaaag tggaactaaa tatgagtaat gatgtagcat acttggataa tataattact
    38641 tacaagccaa atgtaaataa tatatatggt tgtcacaatt tttatcctca aaggggtaca
    38701 ggcttgccat atgatttttt tgtaagatgc tcaaatagat ttaaaaagta tggaataaga
    38761 actgctgctt ttgtcaattc aaaatacgca acaatcggac cttggaatat aaatgatgga
    38821 ctgtgtacgc ttgaaatgca tagaaactta gatattgcta cagcaactaa gcacttattc
    38881 gcaacaggat tgattgatga tgtaattgta ggaaatgcat atgcaagtga agatgaatta
    38941 aaaagaatgt ctgaagtaaa tgaataccaa gttgtattta atgtagtagc taaagaaaaa
    39001 acttctgaag tagaaaagat aataatggta aatgagcaac actttagaag aggagatata
    39061 actaatcagg tagcaagatc tacagaagta agaaaaaaat atgctaaaga aaatttccct
    39121 aaacatgata ataagaacat ctttaaaaga ggagatgtag tgattggtaa tgatgacttt
    39181 ggaaagtata aaggggaatt acagttgata cttcaagatt gtacagatga aagaaaaaat
    39241 aaggtgggta gaataaagga agacgaaata atacttttag actttattga accttggagt
    39301 aaatttaaga ttattttaga gggagagtaa agtgaagata gacatattaa taaaatgtgg
    39361 aaaaacagta gataaaagta taattgatat agctatatta gacgataaaa taatagaagt
    39421 aaaaaatcat atagatgatt ctaagtatca ggcaaaacaa attataaact tagatggaga
    39481 gaaatttatt agtgcgggtt ggatagatat acatacacac tgctacgaaa gactagacct
    39541 ttataaggat tatccagatg aggttggaat aaaaagtgga gttactactg tagtagatgc
    39601 aggaacaaca ggagctttag atataggtga attttatgaa gatactaaaa agtataagac
    39661 caatgtatat gctttaataa atattgctaa gcagggaata acatctcaag atgaactttc
    39721 aaatatgatg aatattgatg aatatgaact taaaaatgca gttagaaaat acaaagattt
    39781 tgttgttgga atcaaagcta gaatgagtaa aagtgttgta atttcaaatg atgttgaacc
    39841 tttaaaagta gctaaaagaa taaaaaatga acttaatcta cctatgatgg ttcactttgg
    39901 gagttctcct ccaacgatag aggatatatt tgattatatg gaaaaaggag atatattaac
    39961 ccatatatat aatggaaagc caaatggaat acttagaggc aatgaggtta aaaaggaaat
    40021 catagaagct aaagaaagag gtataattct tgatgtaggt catggaaccg aaagttttag
    40081 tatggatatt gcaatgaaat ctaaggatgc aggaattttt ccagacacta taagtacaga
    40141 tatatatatt aaaaatagaa taaatggtcc agtgtataat ctatctacaa caatggaaaa
    40201 gtttatatat atgggctatt cacttgaaga tataatagat aaagttacta aaaatgcagc
    40261 agatgcaata agtttaaaaa ataaaggttt aataaaagaa ggatatgatg ctgatttaac
    40321 tatatttgat gtggttaatg aagaaaaaga acttcaagat tctttgaata agtcagtaat
    40381 cacaagtacg tctataaagc caagagcagt agtagtcaat ggagaatatt taaatatagg
    40441 caactctata gaagagaatg agtagaaggg agaaactatt gtatgagtag tatatttgaa
    40501 aaatactctg taagacgtgt tataaatgct tcaggcaaaa tgactatact aggagtatca
    40561 acagtagata aggaagttat tgaagcaata ggtgaagggt gtaaaaattt ccttataata
    40621 gaagaacttg taaataaaac aggtgaatat atagcaaatt tattagggtg tgaaagtgca
    40681 ctaatagttt ctagtgcatc tgctgggatt gcacagagtg tgggagcaat gatagcaaaa
    40741 gatgatatga gtctagttta caatgtaaat aatcctaaga aacaagttaa aagggaaata
    40801 ataattccaa aggggcataa tgttgattat ggagttccag tagaagtaat gataagactt
    40861 ggaggaggag aagttgttga ggccggctat gctaatatgt gtaacaagga acatataata
    40921 agagagataa atgaaaatac agcagcaatt atgtatatta aatctcatca ttgtgttcaa
    40981 aagagtatgc taagtgtatt agatgcagta caagttgcta aagaatataa tttacctttg
    41041 atagtagatg cagcagcaga agaagattta aatgtatatt atgaattggg agcagaccta
    41101 gtgatataca gtggtgctaa ggctatagaa ggtcctagct cggggttagt aataggtaag
    41161 aaaaaataca tagataatgt aaagcttcaa tcaaagggta taggaagagc tatgaaaata
    41221 ggaaaagaaa atattgtagg tctaacatgt gcaattgaga gatatataag taatgataaa
    41281 gtaactttaa aagaaatgga ggacaagcta aatccattta tagacaagat aaatactata
    41341 aagggagtga gtgctagtat aactagagat tcagcaggta gagaaatact tcgtggagaa
    41401 attaacttta atgaagatat aataaataag tcaacacaac aaattataaa tgaacttaga
    41461 agtggagaga tagctatata cactagagac tataaggcta atgaaggcaa gattgagatt
    41521 gatataagaa gtgtaactgg aagtgattta gataatattt ataataggat aaaaaatata
    41581 gtagaaagtt agagtgtaga gtaagtgaca ttataatgat ttaataaatt gaattttacc
    41641 aatggagggg ataaatatga attgtatacc acaatattat aaaaatagag tttgtttaaa
    41701 tgttcttgca ggaagcataa aaaatgctaa agaagtatat gatgcctgtg atggacatgt
    41761 actagtagga gtactatcaa agaattatag cactgtagaa gcagctattg aagatatgaa
    41821 aaaatactca aaggaaatag ataatgcaat atctgtgggt cttggagctg gagacccaaa
    41881 tcagtcaaat atggtatctc agatttctaa agaacttcag ccacaacata taaatcaagt
    41941 gtttacaggt gtcgctacat ctagagcatt gcttggtcaa aatgatagta taataaatgg
    42001 attagtttct ccaactggta aaccaggatt tgtaaaaata tccacaggtc cattaagttc
    42061 aaaagaaaaa gatggtatag ttcctataga aacagcaata gctatgatga aagatatggg
    42121 aggaagctca attaagtttt ttccaatggg agggcttaaa acaaaggatg aatatataga
    42181 agttgcaaaa gcttgtgcaa agcataattt ttatttagaa ccaacagggg gtatagactt
    42241 agataacttc aaagagatag ttcaaattgc acttgatgct ggtgtagaaa aagttatacc
    42301 tcatgtttat acatcaataa ttgataaaga aacaggtgat acgaaagtgg aagatatagg
    42361 agtactttat aaaataatga aagaattatt gggatagatt tatgaatata ttagcttttg
    42421 gtgaaataat gatgagactt tcagtacctg actacaagtt cttaacacaa accaatgaac
    42481 taaattatat aattacaggc acgggattaa atatattatc tggacttaaa aactttggat
    42541 acaataccta tatgttgaca aagttgccta ataataatgt aggtaaagca tcaagtgcaa
    42601 atataagaaa gcttggagta aaagatgact ttatcacata tggagggaat catataggag
    42661 tgtattttct tgaaaatggg tatggagaaa gaccttctga agtgacttat ttgaataggt
    42721 taaatagttc attttgtgag agtaaattac aagattatga tattgataaa tgtttagaag
    42781 aaatggatat agtccatatt tgtggtattg ctatgcaact tacagattca gttaaaaaga
    42841 ttgctttaga actagctaga aaatcttatg aaaaggggat aaaagtattc tttgatttta
    42901 attttagaca atcattaaat ccagaaagga cttatgatga tttagtaaat gattataaga
    42961 aaatactacc atattgtcat gggctgtttg ctagttatag agatataaat aagatactta
    43021 atatagatag tctaaatata aaatctagtt cagaattgat taaaaaagag tatgatatag
    43081 atattgtagc tggaactatg agaagatttg atgatatgaa taatagattt ataaagggat
    43141 atatatcaaa tgatgatggt tattttgaaa gtagggagta taagttagaa atatatgaca
    43201 gagttggtgc tggggatgga tatgtggcag gaattatata ttcatatttg gaaaatatga
    43261 agagtgaaga tatagttgag tttggggttg ctagttcggt gttagctcat acaacttatg
    43321 gggataattc tttagtaagc acaggtcatg tacttagact gatgaacaat gaaaaaatag
    43381 atatattgag ataatacatt tgtatataat aaaattttta tcttttttag acacactttt
    43441 gacttgctta tatttgttaa acagagttat ttttaacgat tataaatatt aaattaaaag
    43501 ttagataata aaatagtaga aaatagcatt aaccatataa aaatggttaa tgctattttt
    43561 atagttaata ttgattaatt ttatattata gaaatgaact tttctaatgc tggatttgta
    43621 ttttttttat tccatacaac cacaacttca attctatttt ctacttcttt tataggtact
    43681 attgcaatat cacgttctat cattgtatct gtaacacaaa atgaaggtaa aatggatatt
    43741 cccatgttag aagataccaa tgattcaata gtattaagat tatttgaacg ttgtaccaca
    43801 ttaggtacaa accccgcttc acgatatcta ttttgtatca ttgacttttg tccatactca
    43861 tcccctgttt cttttacaaa aataaattta tcatgttgta attcaattaa atcgatagaa
    43921 tcctcattgt aaagtggatg accaggaggt aaaagagcta ccaaagaata tctttctaca
    43981 gttttatatt caaaatcttt taatggttga ttttctggat taataacaaa agcgaggtcc
    44041 atagaattat tttttactaa gtttaaaagt tcggttattt cattagtaca aatatccata
    44101 gatatattag ggtatgtatt agaaaattct tttaagtatt gttgaagttt gtttttttca
    44161 tatcctatta aggttccaat gctcaaagtt ccaaaaaaac catgtgcaaa ttgttgtgtt
    44221 tttgcaatag catcattgat attttttatt atagtacgtg cctcagataa aaaaactttt
    44281 ccggctggtg ttagttcaac atgacgttta cttcttgtaa atagagttac atttatttgt
    44341 tcttctaaag cttttatttg ctgtgtaaca gcagtttgag aaatataaaa tttttctgct
    44401 gttttggtaa aacttagatt ttctgctaca gatataaaat attctaattg tttagtattc
    44461 aaataatcac ccctatttaa acttgattgt ttgtaaacct tatttatcta taattaaagc
    44521 ttattatagt ataagaaaat ttacttttaa agaatattag aaaatatttg tataaaaata
    44581 tatgttttaa gtatatcttt acattagatt ttataaattt attaataaga ataacttata
    44641 tattccaatg aaaacgatat tggatttaag taagtttgtc tcataaaatg aaagtatatc
    44701 aattgataat actttaaata agaagggaga aaaaatggaa gataaatttt atgcaaaagg
    44761 caacggaaat aacggatata ttaaaaatct tgaagtttgt tcctttaata acttagatgg
    44821 aacttgtgga atgtttcaaa tggctctgta caaaagagat gaaaaatact atttatatgg
    44881 atgctgtttt ggaggaaata aaaaaaatgg agtaatgatt agcgatatta cagaccctta
    44941 taatccacaa tttataaaac attttcaaat gttagaccct aaagagtatc ctacaacaac
    45001 aactccaaaa attcaaatag cagatgattt aatgatagta gcaatgagtt gtggaagtgg
    45061 accaggagca cttgttgacc aagctaaatt agcaaatatt aagtgtgaag caggaattag
    45121 aatatacagt ttaaaagaag accctttaaa tcctaagttt ttaggatatt gggattgtgg
    45181 cttaaagcat gtaatgggtg ttcatagatt catgtacaat ggtggaagat atgtacattt
    45241 atcaagtgat tgtgttggct ttgaaggtct gatttatagg gtcatagata taataaatcc
    45301 taataatcca gtggaaatag gtaaatggtg gagaccagac caatatgcag atggatatcc
    45361 aaatagaact tttgatgcag gagcacctca ttgcccagaa tttatggata aaggatggct
    45421 tcatggacct ccatttgtaa gagacggaaa agcatattgt ggttatggag gagctggttt
    45481 agttgtatta gatgttgaag atttaacaag accaagatgc ttaggtgaat tgccatttat
    45541 gcctgcattt tctagtagac ttgcaggtgc aagaactcat acagcattac cattgccagg
    45601 aagagattta gtcgttgttc aaaatgaggg agaaagattc cagttcttta aaccagataa
    45661 cattacagat gttcaagcta tgaataatat acatatggtt gatgttagtg acccaacaaa
    45721 accaacatta attgctcaat tcccatatcc tgaagttcca aaagatttcc cttatcctaa
    45781 ctttaatgtt gcaggattag gaaaagcagg gccatttggt ccacataatc ttcatgaacc
    45841 aatggataat aagccatggt tagagcaaag aggagataga gtatattgct gttatttcca
    45901 tgcagggcta agggtttatg atgtatcaga cccatattat atcaaagagc tagcatattt
    45961 tataccacca aatccaaata aaacaccaga agaatcttat ttcccaggat tcccaggacc
    46021 acgcttggca gtaacagaag atcttatcgt tgatgataga ggctacatca tcatagatgc
    46081 tttagatgat ggattctata tattaaaaat gaaagaagat taaataaata ctaaagaggg
    46141 agagttgaaa atgaaaatac taggtatttc ttttggaaca aaaaatggaa ataatgacac
    46201 aatgtgtaaa gttgcactta aaggtgccaa agaagcaggg gctgaagtag aatttattca
    46261 aatgtcatct attaacatta aacattgtac tggatgttgt gcgtgcgtta aaactttatt
    46321 atccgggaaa ggtagtatgt gtgtattaaa agatgatttt gaatggttgc ttgacaaaat
    46381 gaaagatgca gatggtatag tagtttcaga ccctattttt gaagaagggg catcaggatt
    46441 attccatact ataatggacc gttttggacc cagagcagat agaggaaata acattatagg
    46501 aacaaaagtt gcagaagcta taggaggaaa aattccagac tcaagaatgc ttaatgataa
    46561 agtaatatca tttatgggaa taggaggttc tgactggggt acaagagttc aatgtgaaca
    46621 tgctatgcta gctctcatac caatgtggaa agttatagat aatgcttggt tcccatgggc
    46681 aaaggaatta gtaatggatg atacaaggct tgttcaagtt catcaaattg gtttaaatat
    46741 agttgaagca gcaaaagatt ttgaaaaagc ttcatatcaa ggagaagaag gtgtatgtcc
    46801 acattgccac aatagattat tctaccttga gtcaggtact aaaaaggcaa tttgtgctct
    46861 atgtggtata gttggagagt tagatactat aagtggaaaa actatattta gttttccaga
    46921 agagcaatta ggtcatgctc atgatacttt accaggtaag tttatacatg gtgatgatat
    46981 taagaaaatg gaaggtcatt atgcagaagt tcgtaaaaca caagaattta aagaaagaaa
    47041 aacatcttat aattttatag agccattaac taaagaaaag caagctggag tttcattagg
    47101 ataataatta aaatactaag actctttttt aagggatgtt agatgttata ggaggcttat
    47161 tgtatgaatg aaaagccaag ttttaaacaa attgaaatta ttttagcagt tatttttttg
    47221 gtacttacat attacaagat tggattaact ccagcattta ttgttatatt tataccatta
    47281 tacttaagtt attttgcagt atctatgatt gtaaatcaaa tagcaattaa gctattggag
    47341 ataaggcaag ctagggaagc tactaattac aatataggaa atggatagaa agaaaaaaga
    47401 aatattaaca ctgtctatgg ggattgcttt actacctcct ttatgggcag tattagcacc
    47461 ttatatagga ataaaaactg gagctgtagc actaatctgt gcaggattat atgtaactaa
    47521 tggtaataaa caaaaagatg gattaaaaat aatgtttggt ttttggtgtg gagatttatg
    47581 ggcagtgtta gccattttaa ttatgggata tatgaatttc aatcaaaacc tagaattatt
    47641 tttaacactt agtatattag gtttttttgc agttgtaatt gcatccttat ttgaaaaaat
    47701 aattttccta ccatcatggc tttgtggttg ggctataggt ctaactatta tgacaggtga
    47761 aaatattatt aatttacaag atatttgtat tcaaattgct gtagctatgg cagtaggagt
    47821 atggtacgtg ggtgcaggtg tagatttgtt tacaatatat attttgagta aagataagaa
    47881 aaagggaaat tgaaagcatg tgaatatact ttgattatag gagaatggct atgggttttg
    47941 aatttaaaat aatgagaagt ttaatatatg taggacttgc caaggaagaa tatagaccta
    48001 agttaatgga ctggttatat cgtcaccata ttccagatag tattagcact tttggaccat
    48061 attgtactaa atatgccttt tatcaagcat atcctacacc aaatgaaggt gagcgttttg
    48121 gtgcacgtaa gatgcaacta acagaacatt attggcttgt agatgaacat atgcctgaga
    48181 tggcaaatag aattatgaca gaatatatgc ctatggatgt tctacgttgg caagggtgta
    48241 taccagatgt agaaaataaa agggttcatg aaaatgcaga aagtggagat gcaggacgtg
    48301 cagtaggtgg agataatgga tgcccaccat ttatatttgc ttttgttcca ataaactggg
    48361 aagaagactt tagaggaaaa ggacgtactg tacaagatgg accaaactat cgttggcaat
    48421 ttatgattaa gtatccagat ggtatctcta aagaagaagg agaaaaatgg ttctatgatg
    48481 aggtagtgcc atactttaca aactgttgct atgttaatcg ttttgtcagt agtaaaataa
    48541 tgattaatta tggagcaact gcttttgacc gtgtatcaga actatggttt gaaggggaag
    48601 aggaatggta taaagctgtg gttgaagaga caaagtcgtt tattaaaaaa ccagaatggg
    48661 cacaagaaga ggagttccca tatttaaaac cacaattcaa tatcgcatca gtattcttag
    48721 gtgatatagc aactatggat gcatactcac agtatcgtgg atatatacca atgagataaa
    48781 aattatagtt tgtgataagt agaggaagta cttactctta ctgctagaag aagacttatt
    48841 gtacacttaa aagaatgaaa tagataagtg tcaatttatt atatgagaga aactttaact
    48901 ttcttggttt aaaatattaa agtcataagg aacatttatt attaaatact ttatttatat
    48961 gaatt
//
LOCUS       DAEPLF010000008        43678 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEPLF010000008 DAEPLF010000000
VERSION     DAEPLF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43678
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.008"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..1872
                     /locus_tag="KR314_000436"
     CDS             <1..1872
                     /locus_tag="KR314_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBH0326842.1"
                     /translation="IDSSTIVKFFKDGLDKKFNSASIQLKNLYSYDKKLYGYCSVLRL
                     FKNIDFRYHGKVHEQPIYKNPIFNNVADFEHYGYLFEDEEIRLNKVKRNEKLLFEELK
                     ENDNSPYTNYQLGKNFIILGKYHEALYYLEKSYELYSKLESAPGYLITSLVKTYLYLG
                     KHKKCEKLCLKYIKKDMNNIDIYYYIAQAQVDLGKYENSIDSYKRYIYLLDNYEISTQ
                     ANSLFSDTDTVGLRDEAIITLIKIYYKLEKYESVISEYNNIEDMEKKKNVYFSLFMSL
                     YKLNRFEDIKNYYKEVSNSKIEKNSFYKNIEEVINNIKDDEKEHVYKILCDIEGNYGI
                     LNQIRINKCVSIDKCKEILNEEKEIIYAYIIKVAFEDKIDLLDIFYEMEYVWIEKYLK
                     YLLALERSFNLVLYKYIISRPNTSDIKKIRVYKAISKVVLESISLSDEKYKEIFYLYI
                     MYSYKYIRYIYRNLSDFELIKFVCNDLDKFTIEFKHLLDIKNKVNDANEKLEYIHSMR
                     KLLNEYPFYNKIIKILISELEENIKESEEFKDLKDSFIISIENMIETGKINDAKSLVD
                     DYSKTFMDDVKILNIKGILYMFENKLDKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            1896..3773
                     /locus_tag="KR314_000437"
     CDS             1896..3773
                     /locus_tag="KR314_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0326843.1"
                     /translation="MNTPIVVNEKSKDIIKDWQYTYTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFMFAHKINTSNINLWKYRLKLVGFCMLIKKSVLDNVGVLDELFTPGNFEDDDLSFR
                     MLESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIADIEKVKLDYEKDFFDYIILGDVLEHLTDPWKIVNELKQYLKKDGCLISSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSVTATSMPLSEID
                     NRFIDTMCKESCKEMRQQYESYQYIVKATKSIDTKRYSSISMVNLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLVDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSKKVQSESDIQDILYFIEGKRY
                     E"
     gene            3766..5880
                     /locus_tag="KR314_000438"
     CDS             3766..5880
                     /locus_tag="KR314_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBH0326844.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDAFIINKNFIYDILEIFKKNTDVGMIGVAGVKDI
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVANINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLDIVSKIAHFS
                     VFNHTGLYSCEKCERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAIDDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKEKQSEIKHFVIDKHCIEIKNYIDKIYKEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            5889..6884
                     /locus_tag="KR314_000439"
     CDS             5889..6884
                     /locus_tag="KR314_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBH0326845.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIIYKGEEILAVVPACETYENGERVFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLETYLQEQSFNKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKK
                     DILANFRYSKRRYFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            6976..8208
                     /locus_tag="KR314_000440"
     CDS             6976..8208
                     /locus_tag="KR314_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBH0326846.1"
                     /translation="MKRLMILGSIKYFENIVMSAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEIFDENNENMIENF"
     gene            8228..9172
                     /locus_tag="KR314_000441"
     CDS             8228..9172
                     /locus_tag="KR314_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBH0326847.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSDIFLYNMKDAKLKAILDGNYITA
                     FRTAAVAGLAIDTFAKKDFNTISIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFIKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYNVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLNCEEIELKEPKEKYWI"
     gene            9266..9673
                     /locus_tag="KR314_000442"
     CDS             9266..9673
                     /locus_tag="KR314_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HBH0326848.1"
                     /translation="MDKYNLIKFLSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKESIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNM"
     gene            9760..10851
                     /locus_tag="KR314_000443"
     CDS             9760..10851
                     /locus_tag="KR314_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBH0326849.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVRHFEEE
                     FASYVGSKYCVGLASGLDALIIAFRALGISEGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASKMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKNGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFVGMEDEEVSYIIKIINEY"
     regulatory      11160..11250
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            11656..11973
                     /gene="flgB"
                     /locus_tag="KR314_000444"
     CDS             11656..11973
                     /gene="flgB"
                     /locus_tag="KR314_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBH0326850.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYVTFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            11981..12385
                     /gene="flgC"
                     /locus_tag="KR314_000445"
     CDS             11981..12385
                     /gene="flgC"
                     /locus_tag="KR314_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBH0326851.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            12395..12712
                     /gene="fliE"
                     /locus_tag="KR314_000446"
     CDS             12395..12712
                     /gene="fliE"
                     /locus_tag="KR314_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBH0326852.1"
                     /translation="MPITQINNLMQGAKLDNLIGETKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            12737..14287
                     /gene="fliF"
                     /locus_tag="KR314_000447"
     CDS             12737..14287
                     /gene="fliF"
                     /locus_tag="KR314_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBH0326853.1"
                     /translation="MIASFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDVQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAIPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDEDVDENNQPTNSLINVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKAEAKQELEEIKKEGFKKNIIKAVIGVVAVVAIGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            14300..15370
                     /gene="fliG"
                     /locus_tag="KR314_000448"
     CDS             14300..15370
                     /gene="fliG"
                     /locus_tag="KR314_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBH0326854.1"
                     /translation="MSTEITNVNAFDLSDINESDKKEKIEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRDESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPALAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEAAQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            15363..16094
                     /locus_tag="KR314_000449"
     CDS             15363..16094
                     /locus_tag="KR314_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBH0326855.1"
                     /translation="MNNKIIKSKKASYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            16098..17417
                     /gene="fliI"
                     /locus_tag="KR314_000450"
     CDS             16098..17417
                     /gene="fliI"
                     /locus_tag="KR314_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBH0326856.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGIAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            17449..17895
                     /gene="fliJ"
                     /locus_tag="KR314_000451"
     CDS             17449..17895
                     /gene="fliJ"
                     /locus_tag="KR314_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBH0326857.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIESNLE
                     NLESNYSKYSISKNNDSVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            17923..19128
                     /locus_tag="KR314_000452"
     CDS             17923..19128
                     /locus_tag="KR314_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBH0326858.1"
                     /translation="MKIQADSYNIIKDIGNRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRRETSKQNVSSDKRENSDNECHLDEDKSNHLEDENTYEKDCSNNNEALHQLLNLLK
                     DNYKKDKDINTILNNLKKLSISDELKQELKLNKNILNKIANQNLELNRASTNNPNDIE
                     NLDIMKLRELLENNASEDIEVISKTTENDTKSIDLYSFDSNRIDTLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            19146..19754
                     /locus_tag="KR314_000453"
     CDS             19146..19754
                     /locus_tag="KR314_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBH0326859.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEEEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDANSGEENDSSEQESPEEV"
     gene            19781..20764
                     /locus_tag="KR314_000454"
     CDS             19781..20764
                     /locus_tag="KR314_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBH0326860.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGVIYDGEYKADT
                     GEFIKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GNIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            20777..20980
                     /locus_tag="KR314_000455"
     CDS             20777..20980
                     /locus_tag="KR314_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBH0326861.1"
                     /translation="MIRVTDLNGRRYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKVNVGYIRSEK"
     gene            20980..21798
                     /locus_tag="KR314_000456"
     CDS             20980..21798
                     /locus_tag="KR314_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBH0326862.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEVNEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            21788..22483
                     /locus_tag="KR314_000457"
     CDS             21788..22483
                     /locus_tag="KR314_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBH0326863.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGISMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKADSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            22495..22938
                     /locus_tag="KR314_000458"
     CDS             22495..22938
                     /locus_tag="KR314_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBH0326864.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            22953..23327
                     /locus_tag="KR314_000459"
     CDS             22953..23327
                     /locus_tag="KR314_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliO/MopB family protein"
                     /protein_id="HBH0326865.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMNKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            23328..23993
                     /gene="fliP"
                     /locus_tag="KR314_000460"
     CDS             23328..23993
                     /gene="fliP"
                     /locus_tag="KR314_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBH0326866.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            24006..24275
                     /gene="fliQ"
                     /locus_tag="KR314_000461"
     CDS             24006..24275
                     /gene="fliQ"
                     /locus_tag="KR314_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBH0326867.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            24287..26101
                     /locus_tag="KR314_000462"
     CDS             24287..26101
                     /locus_tag="KR314_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBH0326868.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTARMIFFSMDGHHVLIRGIRYSFELIPIGKSFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPVSGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVLKR"
     gene            26110..28185
                     /gene="flhA"
                     /locus_tag="KR314_000463"
     CDS             26110..28185
                     /gene="flhA"
                     /locus_tag="KR314_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBH0326869.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVSKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAEIKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            28160..29176
                     /gene="flhF"
                     /locus_tag="KR314_000464"
     CDS             28160..29176
                     /gene="flhF"
                     /locus_tag="KR314_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBH0326870.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKLIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVVNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            29173..30051
                     /locus_tag="KR314_000465"
     CDS             29173..30051
                     /locus_tag="KR314_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBH0326871.1"
                     /translation="MIDQAQILRKMAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            30065..30766
                     /locus_tag="KR314_000466"
     CDS             30065..30766
                     /locus_tag="KR314_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBH0326872.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELNVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            30782..31204
                     /locus_tag="KR314_000467"
     CDS             30782..31204
                     /locus_tag="KR314_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBH0326873.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEIRSNNHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELSLIRSCYKI"
     gene            31189..31374
                     /locus_tag="KR314_000468"
     CDS             31189..31374
                     /locus_tag="KR314_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBH0326874.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            31415..32188
                     /gene="flgG"
                     /locus_tag="KR314_000469"
     CDS             31415..32188
                     /gene="flgG"
                     /locus_tag="KR314_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBH0326875.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNDYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            32209..32970
                     /gene="flgG"
                     /locus_tag="KR314_000470"
     CDS             32209..32970
                     /gene="flgG"
                     /locus_tag="KR314_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBH0326876.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRIYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNIDGLGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            32993..33874
                     /locus_tag="KR314_000471"
     CDS             32993..33874
                     /locus_tag="KR314_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBH0326877.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMIVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVIKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTKKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            33877..34251
                     /locus_tag="KR314_000472"
     CDS             33877..34251
                     /locus_tag="KR314_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBH0326878.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELNTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            34283..35686
                     /locus_tag="KR314_000473"
     CDS             34283..35686
                     /locus_tag="KR314_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH0326879.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNVKVDDYKNEFLHKSSSNHKIND
                     YNNVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEADVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            35802..37739
                     /gene="htpG"
                     /locus_tag="KR314_000474"
     CDS             35802..37739
                     /gene="htpG"
                     /locus_tag="KR314_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBH0326880.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITIQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            38127..39257
                     /locus_tag="KR314_000475"
     CDS             38127..39257
                     /locus_tag="KR314_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH0326881.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDIDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            39484..40506
                     /gene="splB"
                     /locus_tag="KR314_000476"
     CDS             39484..40506
                     /gene="splB"
                     /locus_tag="KR314_000476"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBH0326882.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            40688..41599
                     /locus_tag="KR314_000477"
     CDS             40688..41599
                     /locus_tag="KR314_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0326883.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYNTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            41614..42030
                     /locus_tag="KR314_000478"
     CDS             41614..42030
                     /locus_tag="KR314_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBH0326884.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(42124..42516)
                     /locus_tag="KR314_000479"
     CDS             complement(42124..42516)
                     /locus_tag="KR314_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0326885.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            42657..43052
                     /locus_tag="KR314_000480"
     CDS             42657..43052
                     /locus_tag="KR314_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBH0326886.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            43455..43661
                     /locus_tag="KR314_000481"
     CDS             43455..43661
                     /locus_tag="KR314_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326887.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFNKID"
BASE COUNT        17924 a         4395 c         7840 g        13519 t
ORIGIN      
        1 attgattctt caactattgt gaaatttttt aaagatggtc ttgataaaaa atttaattcg
       61 gcctcaatac aattaaaaaa tctttactca tatgataaaa aattatatgg atattgctca
      121 gtgcttagac tttttaaaaa catcgatttt agataccatg gtaaagtaca tgaacaacca
      181 atatacaaaa accctatatt taataatgtt gctgattttg aacattatgg atatttgttt
      241 gaagatgaag aaataagatt aaataaagta aaaagaaatg aaaaactgtt atttgaagaa
      301 ttaaaagaga atgataattc tccatataca aattatcaat tagggaaaaa ttttattata
      361 ttaggaaaat atcatgaggc tttatactat cttgagaaat cttatgaatt atattctaaa
      421 ctagaatcgg cacctggtta cttgataaca agtcttgtaa aaacatattt atatttagga
      481 aagcataaga agtgtgaaaa attgtgtcta aagtatatta agaaagacat gaataatatt
      541 gatatatatt attatattgc acaagcacaa gtagatttag ggaagtatga aaatagtata
      601 gattcgtata agagatatat ttatttgtta gataattatg aaataagtac acaagcaaat
      661 agtttatttt cagacactga tactgtaggg cttagggatg aagctattat tacactgata
      721 aagatttatt ataaacttga aaaatatgag tctgtaattt cagagtacaa taacattgag
      781 gatatggaaa agaagaagaa tgtgtacttt agcttattta tgtccctata caaattgaac
      841 agatttgaag atataaaaaa ctattataaa gaagtttcaa attctaaaat tgaaaaaaat
      901 agtttttata aaaatataga agaagttata aataatatta aagatgatga aaaagagcat
      961 gtatataaaa tcctttgtga cattgaaggt aattatggca ttttaaatca gataaggata
     1021 aataaatgtg tatctataga taagtgtaaa gaaatactca atgaagaaaa agaaataatt
     1081 tatgcatata ttataaaagt agcttttgaa gataagattg atttactaga tattttttat
     1141 gaaatggaat atgtatggat agagaaatac ctaaaatatc ttttagcact agaaagaagt
     1201 tttaatttag tactatataa gtacataatt agtcgtccga atacatcaga tataaaaaag
     1261 ataagagtat ataaagctat atctaaagta gttttagaaa gtataagttt aagtgatgaa
     1321 aaatataagg agatttttta tctttatata atgtattctt ataagtacat aagatacata
     1381 tatagaaatt taagcgattt tgaattaata aaatttgttt gcaatgattt agataagttt
     1441 actatagaat ttaagcatct acttgacatt aaaaataaag taaatgatgc taatgaaaaa
     1501 ttagagtata tacatagtat gaggaaatta ctaaatgagt atccttttta taataaaata
     1561 ataaaaatac ttataagtga attagaagaa aatataaaag aaagtgaaga atttaaggat
     1621 ttaaaagata gttttattat tagtatagaa aatatgattg aaactggcaa aataaatgat
     1681 gcgaaatcat tggtagatga ctattcaaaa acatttatgg atgatgtaaa aattttaaat
     1741 ataaagggaa ttttatatat gtttgaaaat aagcttgata aagctgattt tatgttaaaa
     1801 aaggcattat cattagatgt agaaaatgaa gatactatat ataatataga atacttaaaa
     1861 agtttaagat aagtcaaaaa tgggagaatt gaagaatgaa tacaccaata gtggttaatg
     1921 aaaaatctaa agatataata aaagattggc aatatacgta tacaaacgaa gatataaaat
     1981 ttacaagcat aataatattg acatataatc aaatagaata tactaaatta tgtattgaaa
     2041 gtattaggaa gtttacccca aagaataaat atgaaataat aataattgac aataactctt
     2101 ctgatggaac agttgagtgg ttaaagggac aatctgacat aaaatctgta tataataaca
     2161 aaaacctagg atttccaaaa ggatgcaatc aaggtattga gttggctact ggtgaaaaca
     2221 tattgttgtt aaataatgat gtaatagtta ctccaaattg gctttataat ttgaataaag
     2281 ctttatggtc tcaagataat ataggtgctg taggtcctat tacaaatgca tgttcgtact
     2341 atcaacaaat tgatgttgat tataaatcaa ttgaggatat gtttatgttt gcacacaaaa
     2401 taaatacatc taatattaac ctatggaaat atagattaaa gttagttgga ttttgcatgt
     2461 taataaaaaa atctgtgctg gataatgtag gggttttaga tgaattattc acacctggaa
     2521 attttgaaga tgatgattta tcttttagga tgctagagtc aggatataag ctattattat
     2581 gccaggatac ttttatacac cattttggaa gtgtatcttt taaagaaaat cgtaataaat
     2641 atacagatat aatgtatatt aatagaagaa aatttgaaac taagtgggga tttaatccag
     2701 aatatagtgg aatgataaga acagatttga tttctaaaat agatgatagt ttggaggcaa
     2761 atattaatgt tttagaaata ggctgtggta taggggctac tcttttagaa ataaaaaata
     2821 tatataaaaa tgcatcaata tatggaatag aaattagtga gtcagcaggt aaacttgcaa
     2881 agtatagtgc agatgtactt atagcagata tagaaaaagt aaagttggat tatgaaaaag
     2941 atttttttga ttatataata ttgggagatg tacttgaaca tttaactgac ccatggaaga
     3001 tagtcaatga actgaaacaa tatctaaaga aggatggttg tttgatatct agtataccaa
     3061 atattatgca tgtttctgtt ttaagaaacc ttgtgttagg taattttaca tatgaagatt
     3121 caggaatttt agacaagact catttaaggt tttttacatt aaatgagata acaaaaatgt
     3181 ttaatgatag taattacata ataaatagtg ttacagctac tagtatgcca ctttcggaaa
     3241 ttgataatag attcattgat acaatgtgta aggaaagctg taaagaaatg agacaacagt
     3301 acgaatctta tcaatatata gtaaaagcca ctaaaagcat agatactaaa agatattcta
     3361 gtattagtat ggttaattta aaatacgctt taatgagaat tgataatgaa ttagatgtag
     3421 atagtaattt aagttatata tttgataatt ataatataac tgaaccatgt tttgttgatg
     3481 atattgaata tttagttgat acgtttgtga taaataaagc aactctatta aatagattag
     3541 gagttgaggc atttaacaga ggtttaggag attttagtat aaacatgttt atgacagtat
     3601 tagagatgga tagagatgat attgattcaa tatataatat aacaagtgta ttatgtgaat
     3661 tagaagaatt tgatactgct tacaacttaa ttaaaaatag tagcaaaaaa gtacagagtg
     3721 aaagtgacat acaagatata ctttatttta ttgaggggaa gagatatgaa taatgataag
     3781 atatgtttta taacctgtgt aaatgatgaa agactttatg aggaatgtct tgtatatata
     3841 aatagcctag acattcctaa aaactttagc atagaaacag tagctttaag aggagccgat
     3901 tctattgcct ctgcttataa taaagctatg agagaaacag attctaagta taaggtatac
     3961 ttacatcaag atgcttttat aattaataaa aatttcatat atgacatttt agagatattt
     4021 aaaaaaaata cagatgtagg aatgattggt gtagctggag taaaagatat acctgtgaat
     4081 ggaatttggt gggattctga taaaaaagca gggaaagtat atgatagcca tacaggtaaa
     4141 atggatattt atgaatttag taactttgaa gaatgtttta atgaagttat ggctatagat
     4201 ggattaatta tgattactca atatgatgta ttttggagag aggataagtt tgatgggtgg
     4261 catttttatg atttgtcaca gagtatggaa tttagacaaa ataaatacaa agttattgta
     4321 ccaaaacaaa ataagccttg gtgtatgcat gattctggag tagcaaatat aaataatgga
     4381 tatgatgaat ataggataaa atttgtaaaa gagtacataa atcttgattt atatagagaa
     4441 aatttttatg aatttgataa gaagaaagca agtaccatac ttaagagtaa taagcatgta
     4501 atggattgta tgaaagagga gcttacagaa caattaagat taaagaatta taaggatgtt
     4561 ttagatattg tttctaaaat tgctcatttt tctgttttta atcatactgg cttgtattct
     4621 tgtgaaaaat gtgagagagc attgctagaa tgtgcaaata atttaccaga aatagaaaat
     4681 acgaaattat ttacaaaaaa agatttaaaa agaagaaaag tattacatgt tttaagtgaa
     4741 ggatattcaa caggagggca tacaagacta gttaaaaatt ggattgagat tgacaaagat
     4801 tctgttcatt cagttgtaac tacatggcaa tcagatacac tgccagactg gttattaaaa
     4861 gcaatagatg atagtggtgg cgaagttata tctttggatt taatttcaga tgattattta
     4921 aaaagagctg tagctttgag gaaaatagct tatgactggg ctgatatagt tattttatat
     4981 gttcatatgt gggatacaat acctgttgtt gcttttggag ttgatgggaa aatacctatt
     5041 ttacatgtta atcactatga tagtactttt tggttaggga caagtattgc agatattgtc
     5101 attgatttta gcgaagctag tcaattaatt acattagata aaagaggcat agataaatct
     5161 gaaatattac caatgccatt aaaagaaaaa gaagtatttt caaaatctga agttagaaaa
     5221 cgatataatt tggaggatga tgtgacaata atattgacta ttgctagtga aagaaaatac
     5281 aatagtgtaa atgatattaa ttactttaaa attgctaaaa cattgattga aaatacagat
     5341 aatacaatta ttattgttgt aggagttgat acagagaatc tctattggaa aactttaagt
     5401 aacgttacag ataatagagt tatatgtttt ggaacaacag caaatataga cgatttttat
     5461 aagatttcgg atatatacct agagtctttc atggtaggga gtcatacttc aaaattagat
     5521 gcaattttat atgatttaat tcctgttaag tttaaaaatg aagtttcacc aatttttaca
     5581 aatatgtgga attttataga taagtttgat tataagaata taaaagaggt tatagacatg
     5641 ataagaaggt acaatactag tgaatttgac agggataaga taaaagaaaa acaatcagaa
     5701 ataaaacatt ttgttataga taaacactgt attgaaataa aaaactatat agacaaaata
     5761 tataaagagg ctaatcatca taaagttaag tatgatttat ataaaaatga tgaaatcgaa
     5821 gactatcagc tttttgttgc attatatggt tatatggtaa aaaataaaaa catgtcatag
     5881 gtgataaaat gaacaatatt catatcaata aatttgaaaa aagctgtgaa aaaaactggg
     5941 attattttgt agaaaataaa tctataaatg gtacatttct gcaaactaga aatttcctta
     6001 attatcatga agaaggaaaa ttcactgacc actcattaat aatatataaa ggtgaagaaa
     6061 tactagcagt cgtacctgca tgtgaaactt atgaaaatgg tgaaagagta tttttttctc
     6121 atttaggaag cacttttgga ggtattataa taagaagtgg ttttaatgat attaggcatg
     6181 taaatgctat tacaaataag ttagaaacat atttacagga acaaagtttc aataaagtat
     6241 atttaaaaaa tacatcaaga atattttgta aagaggatac ttcattacta gaatattttc
     6301 tatttaagaa taattacatg ttttatgatg aattaagtat gtttttagaa ttaagtaagt
     6361 ataaaaaaga tatattagct aattttagat atagtaaaag aagatatttt aaatattctt
     6421 tgaagaataa tcttaccttt aaaagattgt atagtgatga ggatatagtt tgttttttta
     6481 atttactttc taattgttta gatagtaaag gagctaaagt aatacattca gtagaagaac
     6541 ttttggagtt taaaaataat agattaaaag atattgtaga attttatgca gtatatattg
     6601 atgacattat gatttgtggg agcatggtat ttaactttca agaaagagtt tatcatactc
     6661 aatatctagc atcaaattcg acatataata aatattatcc aatgatattt ttatactata
     6721 atctaataaa agatgcttta gagagaaatt ttaagtatct ttcttttgga attagcactc
     6781 taaatagagg caaatactta gacgagggtt tagctacatt taaagaaggt tttggttcaa
     6841 gtgcaatttg caacagaaca tactataaaa atataaataa gtgataatca tgttttatag
     6901 agtttttaga gtatagtaaa tatctagtac tctaatatta cataaaaata attagtttat
     6961 aaagaaggtt tgtatatgaa aagattaatg attttaggct caattaagta ttttgagaat
     7021 attgttatgt cagctaaaaa agagggaata tacacaattg tttgtgataa tagaattgat
     7081 actcctgcga aaaagatttg tgatgaagca atagatgttg atgtatttga ttttgataaa
     7141 ataaaaagta tagctataga taaaaagata gatggaattt taactggatt tacagattct
     7201 ttaatgaaac catatgttta tattgcaaat gaactaaatt taccatgtgt gatttcttgc
     7261 aatcaacttg aatctgtaac taataaggct attatgaaaa aatattttaa aaataatgca
     7321 atacctacaa ctgattattg tattattgaa agtctaaaag atttagaaaa aatacaagga
     7381 ttaaattttc cacttgtaat aaaacctgtt gatggttatg gttcaagagg tgtatatttt
     7441 gtatcaaata tagaagaatt aatagaaaaa tttgaatatt ctaaggaagc ctcacaagat
     7501 ggacaagtaa taattgagga attttatgaa agtgaggaaa tacaaggact agcttgggtt
     7561 cataatggag agtcacatgt attttatatt ggagatagag agcttgtaaa tatacataca
     7621 ggaagagctg gaaaaccaga tagattactt tatccatcaa agtattgttt tcaatatgag
     7681 gaagaaataa aatctatata tcaaaatata acacaggctt ttgatataaa aaatggacct
     7741 ctttatgtac aaatgttagt gggagcatat ggaataaagg tatctgaagt aatgccaaga
     7801 ttacctggag gttgtgacta tatggctatt tctcaaataa cagatttaga cataggaagt
     7861 ttatttgcaa actttagtgt gaataatgaa gtaaattttg atgaaataaa gaaatataat
     7921 atgcatttat caaaatgtgt atatgcatta ccaatataca taagacctgg aacaataaaa
     7981 aagataaata atatagataa aatagaaaac ttagattatg tcactcaatt tctattatta
     8041 gtaaaagaag gagatacaat aggattatat gaggatgaga gacaggattg tggaagagtt
     8101 tatggaatag caaataatat atttgatgca aatataaaga agaaatgtat tcatgaaatg
     8161 atagaaatat ttgatgaaaa taatgaaaat atgatagaaa atttttaaat aagggagtat
     8221 gatagatatg aagctaataa ctcatgaaca aattatgtca ttaaacattt ctcagaaaca
     8281 aggatatgaa tggataaaag aatctttttt aacaaaaaaa gattgtcaat taccaccaaa
     8341 aataagcatg gtagaagaag gtcatgtatt ttacaatgta atgccgtcct taaatcataa
     8401 ccatatgatt ggtggaataa aaatggttac aagattgcca gggagaaatc caagtttaaa
     8461 ttcagatata tttctataca atatgaaaga tgctaaatta aaggctattt tggatggtaa
     8521 ttatattaca gcttttagaa ctgctgctgt tgctggtttg gctattgata cttttgctaa
     8581 aaaagatttt aatactattt ctattatggg tttaggtgta actgcttctg ctataatgaa
     8641 tatgctttcg tatactctta aagataaaaa tataaatata aagttactta aatataaaga
     8701 ccaagcagag tcatttataa aaaagtatga aaaaaatgaa caatttagtt ttgaaatatg
     8761 tgatacacat gaagaactta ttgtagattc agatgtagtt atttctactg tgacatatac
     8821 agatgaatat ttatcagaat ttaaatggtt taaaaaaggt gctttattat tacctgtaca
     8881 tgttatggga tttggaaatg ttgattatct atttgataaa gtatatttag atgattataa
     8941 tcatttgaaa catctagaaa atatagatag atataaatat tcttgtgaat tttataatgt
     9001 tttaagtaaa caagctaaag gaagagaaaa tgatgatgag agaataatat cttacaatat
     9061 aggtttgtca atacatgata tattatttgc aaacaaaatt tacgaattgt gtgaaacact
     9121 aaattgtgaa gagatagaat taaaagaacc aaaagaaaaa tattggatat agtttatttc
     9181 attcatattt acaataattt attgtaaata tgaatatgaa gtaatatatt gttagaaaat
     9241 tagcatgaat aggatagata aatttatgga taaatataat ttaattaagt tcttatctat
     9301 tggaaacgaa tatataggaa atttagttgc attagaatca aataaagcaa taccttttga
     9361 tataaagaga gtttattaca tatatgatgt accaaacaat gtaaagagag gatttcatgc
     9421 acacaaaaac ttggaacaag tactagtttg cctaaatgga agtgtgaaga taaaatgtta
     9481 tgatggtgta aaagagagta tatttaattt acataaaaat gatgttggat tgtatatagg
     9541 aacgggagtg tggcatgaga tgtttgactt tgatgaaaaa actgttttaa tttctatagc
     9601 atctgaacat tacaatgaag aagattatat tagagactat aaagaatttt taaagtattt
     9661 aaataatatg tagttattat acagttagtg atgtattttc ttaattaaat aaatttaaga
     9721 tagccatata gagaataaat agattaggat gtgaatttat tgttggttaa atttaataat
     9781 ttattaaaac agttccaaac tcataaagaa gaatatgaac agaagattaa ggatgtaatg
     9841 gaaagtggat attatatcct tggaaatgaa gtaaggcatt ttgaagaaga atttgctagc
     9901 tatgtaggta gcaaatattg tgttggttta gccagtggtt tagatgcact tataattgct
     9961 tttagagcat taggaataag tgaaggtgat gaagtaatcg tgcaatcaaa tacatatatt
    10021 gctagtgtta tgggaatcag cataaatgga gcaacaccag tatttgtaga accaaatgaa
    10081 tattataata tagattcatc taagatagaa gaaaagataa atagcaaaac aaaagctata
    10141 ttagtagttc atttatatgg tcaagcaagt aaaatgaaag atatacaaga attatgtgat
    10201 aagtataact tgtatttaat tgaagattgt gctcaatctc atggagctaa atatgatgga
    10261 aaaatgacag gaacatttgg tgatataggt tgttttagtt tttatccaag taaaaatttg
    10321 ggtgcatttg gagatgcagg agctatagta actagtaatg aggaaatagc tgataaagtg
    10381 aaaattctta gaaactatgg ttcagaaaag cgatattata ataaatttgt cggatataat
    10441 tcaagattgg acgaaattca ggcaggttta ttaagagtaa aacttaaata tctaaatgaa
    10501 attgaaattg aaaaatcaaa aatcgtagat agatatctta atgaaattaa taatcccaaa
    10561 gtcatacttc caaagataga aaataattgt actcatgtat ggcacttatt tgttttacaa
    10621 gttgaaaaac gagatacatt tatggaatac ttaaagaaaa atggtataga gtctataata
    10681 cattatccga taccaccaca tctatctgaa gcatatgctt atttgggata taaaaagggg
    10741 agttttaata tagctgagaa ctattcagat aaagttgtta gtataccttt atttgtcggt
    10801 atggaagatg aagaagttag ttatataatt aaaattataa atgaatatta aactaatcat
    10861 aataatatca aaaataaatt ttctatatat catttattta aatttcgtta attgacattt
    10921 ttaaatattg atatattgta caaataaaat tgaaatatat ggaagaatat agaataaaga
    10981 tgagatttgt ctaatatttt tataaattaa aaatattttc aaagagttta ttataaaatt
    11041 gtagactata gtgtttataa tctttgtaat tattggtaac taaatttaaa aaataaaata
    11101 tttaattata cacaaaaaag atatttaaaa aatttgttat ttattgtata cttaagttaa
    11161 aactaaatag gcaaatctag agaaatctag tgacgcaaag ctataggggc taaggtttgt
    11221 atacacaaac tatgtcagcc agttgccaaa aagagttcta ggtatattgt atacgtagga
    11281 agagtctagg atgactgcct ttttggtggt cattttgtta tggaaaaaat cacattatgt
    11341 agtaaaaaac acctataata acatattata ccaaatataa tttaaaataa agataaatta
    11401 tctataaaag ttactatttt acaaaaatga cttctttatt catccaaatt aattacattt
    11461 ctattacatt aatataataa tgtatcactt tggtaagaaa actaatatta ttttcgtaaa
    11521 tcctaataaa aatataaaaa tattttaata taaatttaaa tatttcttta taaagttgcc
    11581 tttttttgta atatagcaac ttcatttttt attaataagc aaaataaata aaatatattt
    11641 tgaggaggag tgagtgtgaa tacatatgac attataaaaa tgggattaga tgcaactaat
    11701 ttaagaagtc agacaatagc aaataatata gccaatataa atactgaagg atataagaga
    11761 cattatgtaa cttttgaaga aacattaaat gatgctatgc caaaaataga agaaaaaaca
    11821 gacaattcta aaggattaag agtggatggc aataatgtag atatagaaga agaaaaagta
    11881 aatcaagcaa tggcaacatt acaatataat gctttgataa gttttgcaaa tggaaaaata
    11941 gcaatgacta aatcaataat aagtgggagg taattaaaag gtgagtgtgt tcagtggaat
    12001 gagaatatct gctagtggat taacagcaga aagcttgaaa atggatatca tatcgtccaa
    12061 tgtagctaat ataaatgcca caagaggtcc aaatggagaa gcatatagga gaaaagttgt
    12121 ttcatttgaa gaaaactatg ataataaatt aggtatgcta ggagtaaaag ttacatctgt
    12181 tgaagatgat aaatcacctc ttaaagaagt atatgaccca tctcatccag atgcaaagcc
    12241 aaatggatat gtagaatatc caaatgtaaa tatattggta gaaatgacag atttaataac
    12301 agcttcacga gcatatgatt ctaacgtaga tacattgaat gctcagaagt ctatgatatc
    12361 aaaagctttg gaaataggaa agtagggata aattatgcca attacacaaa ttaacaattt
    12421 aatgcaaggt gcaaagttag ataatttgat tggagaaaca aaaccattaa ctcttaataa
    12481 taaagaagaa aaaaagaact ttgaagatgt aataatagat actatagata aagttaattt
    12541 tcaacaagtg acagcagatg atatgaaaga aaaatttata aaaggcgaag atgtttctat
    12601 gcatgaagtt atgttaatag gtcaagaagc acagctatct atgcaatatt taatagaagt
    12661 tagaaataag attcatgatg catatcaaga aatgaataag gtacaaattt agccttttga
    12721 acaggagtaa taatcaatga tagccagttt taaaaataag attaaagagc aagttgctaa
    12781 atctaggaca aagataagtt ctctaaaaaa gggaactaaa atagcattag gtataggggt
    12841 tattgctata tgtttagcgc tagtatttac agcaatatct gcgagcaaaa ataagtatgc
    12901 agtcttattt tcagaactag atgttcaaga tgctgcagat atatcagcag aattagagtc
    12961 taaaaagata gatactaagg taaagggaag tacgatttat gtacctagaa cagaagttga
    13021 taagttaagg ctggagctag caccaagtct tacaggtggt tctaaaggat ttgcactaat
    13081 ggatgatagt tcatcaatag gacttactga tgaagagttt cagattaaga agcaaagaat
    13141 gatacaaggt gaaatagaaa aaacaataaa gagttttcca caagtggaag atactagagt
    13201 acatataaca tttggagagt catcagtatt tgagaaggag gctattccag ggagtgcagc
    13261 agtttatttg atgttaaaac cagggacgac tttggaaaaa aaccaaataa aatctgttat
    13321 ggcacttata tcaggaagtg ttacaaatat accagagaaa aatgttcaag tcattgataa
    13381 caaaatgaat ttattatctg aaggtatttt agaggatgaa gatgttgatg aaaataatca
    13441 accaacaaat agtttgatta atgttgataa aaataaggca tctgaacaag cattgaataa
    13501 acaattagaa aagtctattt tagaaatgct agaaccaata tttggtaaag gaaaagtaaa
    13561 agccacagta aatgctgatt taagttttga tacagctgaa aaaactgaga taaaaattga
    13621 ccctgataaa gttgcaataa aggagacaag gtcgaaaaat tcatctaaat ctgctacaaa
    13681 taatgttcaa ggtgtagatg ctaacatgaa taaccaagga aataacaatg gtactaatac
    13741 agaagatagt ttagatgaga gttttgagta tgaaacaggt aagattgaaa ctcacactat
    13801 agttgcgcct ggagaactta atagattgac agcttctgtt gcaatagatg gaaaaatagc
    13861 taaaggtgta caagctgatg tagaggatat tgttaataat gctataggta tggatggagc
    13921 gaggggagat actcttagcg ttgtttcaat ggtatttgac ccagaaggga aggcagaagc
    13981 taaacaagag ctagaagaga ttaaaaaaga aggatttaaa aagaacataa taaaagcagt
    14041 tattggagta gtggcagtgg tagcaattgg agctattgga tacttaatat ataagaaaag
    14101 aagtgaacga gaagatatgg attatgaaga tgaagattat atttctaaat ctgattctgt
    14161 tgataggaag gataatcctc tattaggaga tgacccagaa ctgacattag aggaagctgt
    14221 taaaatgtat gcagaagaaa agccagacca agtaacagag ataataaaaa cttggttaaa
    14281 tgagtaaggg ggatagaaag tgtccacgga aattacaaat gttaatgcgt ttgacttaag
    14341 tgatataaat gaaagtgata agaaagagaa gatagaaggt gagggtttaa aaaaagctgc
    14401 tgtattatta atgacattag gaccaaatgt gtctagtgat ataataaaaa gcttgcctga
    14461 taaacaagtt caaaaaatag gtttagaaat tgcaaatatt tcttcaatag cttcaaaaga
    14521 aagaagagaa atcttaaaag agtttgtaga gataaatagg gcaaaagatt atgttataga
    14581 aggtgggctt gaatatgcaa gagagttgtt aagcaatgct ttaggaaatg ctaaagcaaa
    14641 caaattactt gaaggtatat caataaatac aagtacaaag ccttttaaac atattataaa
    14701 actagatgta aagaaaatat tagaaatact tagggatgaa agtgcacaaa ctatagctgt
    14761 tttacttgca catatggagc cagaaaatgg agcagaagtt ttatcatact tagattttga
    14821 tttacaaaat gaagtagcta tgagaataag ctcaatgggt tcaatatctc cagaagtaat
    14881 aaaggtaatt gaaagttcat tagaagataa attacactca ataagtggtg atgattttaa
    14941 ggattatgga ggagtgtcta ccttagttca aatattgatt aattctgata gaaaaacaga
    15001 aaagaatatt ttgggaacta ttgaagaaca agacccggct ttagcagctc aaattaaatc
    15061 tcatatattt gtctttgaag atattgtcaa gttagatgat ataagtatac aaaaagtatt
    15121 aaaagaagtg actatgaaag atttagtata tgccttaaag ggtgtggatg atgagatttc
    15181 agatgctata tatagaaatc aatcttctag agcagcagat gcacttaaag aagaaatgga
    15241 aatggttggt gtcataaaag ttacacaggt tgaggctgct caacaaaaga tagcaaatat
    15301 agtaagaaaa cttgaaagag aaggtgccat aactattgta aggaattttg gtggtgactt
    15361 tgttgaataa taagattatt aaaagtaaaa aagcatcata taaaggagtg ctagatttaa
    15421 ccataataga taaagttaga gtgggagaat caaatgtaaa taaagaaaat gattgtagat
    15481 ttagagatga ggtaataaaa gaagctatag aatctgctga gataaaaaag aatgagatat
    15541 tagaagaagc aaaaatgaat gcacaaaata tagaaaaaca agcatatgaa aaagggtata
    15601 gtcaaggtca aaaaaatgga tatgaagatg gatataaaga gtcatatgat gaatataccc
    15661 aaaaagctaa agatgaagca gattttataa aaaatcaggc agacttaata ctattacaag
    15721 ctaatgagaa gatgactgag tacataaaag aaaaaaataa agagattgta agacttgcta
    15781 ttaatatggc tagttctgtt ctaaaaaagc atttcgaaga tgaagaatct atgagtgatt
    15841 tgttaaagca aattattcaa gattatgaag gaagtagttc atttatagta agatgtaatt
    15901 ctttctataa agataatata gagaaagagt ttgaattatt caaagaggaa acatcactaa
    15961 aatcaagtgt atttgttatt gcagatgata gtatagataa aggcaatgct atcatccaaa
    16021 aggaaaatgg aagaataata gttggtatcg attgtgctat ggaaaaacta aaagaagaat
    16081 tgcttggtga ataaagtatg tataaaactg atatatctat agattttgac aaaataaata
    16141 agaaaataga aaatttagaa ctcactatat cagaaggtag agtaaagaag ataataggac
    16201 ttactgtaga agttgaagga ataaaagctt ttgttgggga gttatgtgtt atatacaatc
    16261 aagtcaataa accagtgaat tgtgaagtag tgggttttaa agataaagaa gtcatattaa
    16321 tggcgttggg agagctaaca ttgatagctc ctggttgtaa agttatttct aagggaatac
    16381 ctttaagtgt tatgtgtagc gataatttac taggtaaagt acttgatggt cttggaaatc
    16441 ctatagacaa ctcagatgct gttttaggag atagatacaa cttgaacaat gaacctcctg
    16501 acccaatgaa gagaaaaaag atacgaaata taatggaaac aggtgtaaga gcaatagatg
    16561 catttactac ttgtggtgaa ggacagagaa tagggatatt tgcaggaagt ggagtaggta
    16621 agagtacgac tctaggtatg attgctagga atgcaaaagc agatgtaaat gtaattgccc
    16681 ttattggaga aagaggaaga gaggtactag attttattga taaagattta ggtgaagagg
    16741 gaatgaaaaa atctgttgtt gtatgtgcaa catcagataa agctcctttg gttaggctaa
    16801 aaggtgctct tactgctact gcaatagcag agtattttag agaccaggga aaaaaagtaa
    16861 tacttatgat ggattcagta actagatttg ctatggcaca aagagaagtt ggtatagcaa
    16921 taggagaacc accagctcaa aaaggatata caccatctgt atttgctatt ttaccaaaac
    16981 ttatggagag aacagggaca tcagataaag gttctataac tgcattttat acagtattag
    17041 ttgatggaga tgactttaat gagccaatag cagatacaac aagagggata cttgatggtc
    17101 atatagtttt atctagagac ttggcaaata agaatcatta tccttctata gatgtattga
    17161 acagtttaag cagacttatg aatgagattg cttcaaaaga agacataaaa atagcatctt
    17221 ttgcgagaga tatgcttgct gagtatagag aagcagaaga tttgataaat ataggtgcat
    17281 atgcttcagg tacaaacaaa aaaatagatg aagcaatcta ctatcatgag catattatta
    17341 attttttaaa acaaggaata aatgaaaaga gttcttttaa tgaaactata agtagtttaa
    17401 gaagaatctt tgaataaaca atttaaaaaa atatttgaat aggttgtgat ggaattggat
    17461 aaaaatttta agtttagatt acagaaagtt ttagacttaa aaataaaaga tgaagaagag
    17521 ataaaaatgg aatttgctaa gattcaacaa aaaaaaatag atatagagag caatttagaa
    17581 aacttagaaa gtaattacag caaatattct ataagtaaaa ataatgatag tgttcaaaat
    17641 caaaaaataa ctattaacta tttgttagct ttaaacaact ctataatgga tttatcagaa
    17701 gagctggata agtcgacaaa tgaacttgaa aaagctagaa agcagcttat aagcaaacag
    17761 atagagagaa agtcattaga aaaattaaaa gaaaaaaaat atggacagta ttataaagaa
    17821 gaacagttaa aagaacaaag cactaatgat gagtttgcaa gcatgagtta tttgagaaat
    17881 agacaagttc tataaaatag taggaagtga ggatattatt gtatgaagat acaagcagat
    17941 tcttataata ttattaaaga tattgggaat agaaaaagct catctaagaa taaaaatata
    18001 aaaaataata aatttgaaga taatctatat aaggcaacag accataaaaa ggttaaagat
    18061 agaagagaaa caagtaagca aaatgttagt tcagataaac gggaaaatag tgataatgag
    18121 tgtcatttag atgaagataa aagtaatcac ttagaagatg aaaatacata tgaaaaagat
    18181 tgtagtaata ataatgaagc tttacatcaa ttactgaatc ttttaaaaga taattacaaa
    18241 aaagataaag atataaacac tatattgaat aatctaaaaa aactaagtat atcagatgaa
    18301 ttaaaacaag aacttaagct taataaaaat atattaaata agattgcaaa tcaaaattta
    18361 gagttaaata gagctagtac aaataaccct aatgatatag aaaatttaga cataatgaag
    18421 ttaagagaac ttttagaaaa taatgctagt gaagatattg aagtaattag taaaactaca
    18481 gaaaatgata ctaaaagcat agacttgtac agttttgaca gcaatagaat agatactctt
    18541 aactcaaata aacagaaaga tgatagttct gatattttgg aaaaactagc aggggtaaat
    18601 ggaaataaga gtggaaattt tggacaagta ataaataaaa gcgttgatat gaataaaatt
    18661 aaaaatgata atgttaattt aaaacagata gaatcaaatg taatggatga ttctataaaa
    18721 gctattaagc atatgaaaac aaatgatata caagaattga caattaaatt aagaccaaaa
    18781 gaattaggag atatgaacat acaattgtta aaagatggtg aaagtatgag agctgttgtg
    18841 actgttttca acaaagatgt ctttgactct ataaataaaa acatagcaga tttaaagcaa
    18901 catcttgaac ttacaaatgt aaatataaaa gatgtatcag tgcaaatgca tagcgataat
    18961 acaaatacat ctgatacctt tgataaagca tttgaacaac agaatagaca aaataaccaa
    19021 gaaaacatga gtaagcaaaa tacaaatgat aagcgtaaca atgtagtcat agaagatgag
    19081 attaaagaaa atattattga tgatgataga gtagatttac tagcttagta agggggaagt
    19141 attgcatgta cggaatagca acggctttgg caggaactca ggtaaactat aggtcaaata
    19201 atttaagcca acttgaaaga aatattaatg atattgccaa taaacaagtt agaagtaaag
    19261 gaaatcagac agtaacagac aatgggactc caattatagg acaaggcgat gatgctgata
    19321 aagatttgtt tatgaagatt cttgtagcac agatgggtaa tcaagaccca atgaatccac
    19381 aagacccaac agaatatgta tcacaattag cacagtttgc aactttagaa aagatgtcag
    19441 ctatgaatga taagctagat atacttttag ccttaagcaa ttccactcta ataaattctg
    19501 ctttgagtat ggcaacatca ttaattggta aagaagtaga agtatctggg gaagaggaag
    19561 aagatgaaaa cattgtagga gtagtaaaat ccacatatat aaaagatggg gaagtacact
    19621 tagaattaga agtagatgga agtgacaaaa ctgtaggtgt taaatatgaa aaattactta
    19681 aaataggaga ggtagatgca aattcaggag aagaaaatga ctcttcagaa caagaatctc
    19741 cagaagaagt ataatattaa atgagaaaag aggtaaattt atgataaaag caatgtattc
    19801 aggtgtaagt ggaatgaaag caaatcaaac aaaactagat gtaataggta acaatgtagc
    19861 caacgctgga actacttctt ttaagaaaag ttcagctaga ataacagatt cattttatca
    19921 aactgtacta tatgcaagtg ctccaacagc ggcattaggg ggaacaaact taggacaagt
    19981 aggtgtaggt agtaagatat caagtataaa taaagatatg acacaaggaa atgtacaacc
    20041 aactaatagg ccatctgatt taatgataga tggagatgga tatcttccag tagtaagaca
    20101 aggaacagtt atgtatacga gagatggttc ttttaattta gatacaggat ttaaagcagc
    20161 tgatggtgct ttgaagaata ttaaaggtgg aagacttgta aatggtgatg gatatatgtt
    20221 acaaggtgtg atttatgatg gggaatataa agctgatact ggagaattca ttaaaaaggg
    20281 tgatgctaaa ggaaaagagc ctattgtaat accacttcaa agtataagtc ctaaggatgg
    20341 aacagttcaa aatgtactag agtataatat atcaaaggat ggaacagtag aatttttatt
    20401 gagtgatggt caaagaactc aatttgtcta tgatatacct gatggtgcta aggtaggaga
    20461 tacacctgct ggaatagata aattaaaagg tacaatacaa aaagtgcaaa catatgcatt
    20521 ccaaaatcca gcaggactag atgcagaagg aggaaactta ttcaagccat cagcaaactc
    20581 aggagatcca caaatagcag gagctacagg aaacataata caaggggcaa tagaagcttc
    20641 caatgtagat atagcagaag aatttacaga aatgataata gcaagtaggt ctttccaagc
    20701 aaacagtaaa ataatatcga catcagacga aatacttcaa gaaataataa atcttaagag
    20761 atagtaggta catattatga taagagtaac ggatttaaac ggaagaagat acatattaaa
    20821 ttcagacctt atagtgagaa ttgactctgt accagaaagt aggattctcc ttacaacagg
    20881 agaaaaaaat cttgtaaaag agtcagtaga tgagatagta gataaagtaa taaagtataa
    20941 aagaaaagta aatgtaggtt atattaggag tgaaaaataa tgaaaaaaac agattcacta
    21001 acattcattg gactaatatt atctacagta ttagttttat ttggggcagt aaaaggaagt
    21061 agttcaggat tgaaaagttt tttcgatttt gcatctatat taatcacagt atttgggtca
    21121 tttggagctt tgatgattac atttactatt gatgatataa aacttataaa agatgcgcta
    21181 caatactcat tcaagacaat gaatatatcg aaattagact tattaaatca atttaaaaca
    21241 ctttctaaaa aagcaagaag agaaggactt ttatctatag aaagtgatgt aatgaaaata
    21301 caagacccat ttatgaaaaa agggttagag ttatgtattg atggccttga agtaaatgaa
    21361 ataaggtcta tactagaact cgaaatagat tctgttgaag aaaccaataa cagatattct
    21421 aaactattta aaacttgggg gactttcgca cctgcatttg gtatgttggg aactctaata
    21481 ggtttaatac aaatgttatc tgaccttaca tctccagatt taatagcatc gggtatggga
    21541 aaagcattaa ttacgacatt ttatggttct ttacttgcta atatcgcttt gaacccaatt
    21601 gcatataata tagatgagaa gactgaaaaa gagatttatg taaaggaaat gatgctggaa
    21661 ggtataataa gcatacaatc tggggagagc agcatagtag ttgaggaacg tttagctaca
    21721 tacctttcaa acaatgagaa gctagaaatt atgaaatcca ataaaaatac agaaagagca
    21781 atgagtaatg gcgcgtaaaa aaaataagaa aaaggatgag ataaatccag atgcatggtt
    21841 ggctacttat gcagatacta taacactgat tcttacattt tttgtgttat tgtatgctac
    21901 atcagttgta gatatggaaa aatttaaaaa catagcagat gcgttaaaag gtcagttttc
    21961 tgggataagt atgttcagta caggttcaag tacaagtgag aagtctccaa tagagagtgt
    22021 tttagtggat aaaaatgata agtcaaattt agaacaggat ttaaaagata aagttaataa
    22081 ttctaactta caaaatagtg taacagtcaa agatgatagt agagggattt tactagaact
    22141 agatgattca attttatttg actctgggat ttctgaatta aaagcagata gtaaagccac
    22201 tcttaataaa gtatatgaca tgataaagat gatgaaaaat aagatagtta tagaaggcca
    22261 tactgataat gtacctgtaa aatcaagtac acatgaatcc aattgggatt tatcttctag
    22321 tagagcagta agtgttgtaa gatactttgt agaagaaaag ggcatggacc ctagactatt
    22381 ttcggcaaca ggatgtggag agtatcaacc tttggtttca aatgaaacag caaagggaag
    22441 ggctgagaat agaagagtta atatacttat aactattgac taggaggaag aggtttgttc
    22501 aaaaataaaa aaattattat aattattttg atactgatta ttttctcagc aggagcaggt
    22561 ttttttatat taggagaagg tggaaataaa caaattgaca agattgcagg tttgtttatt
    22621 ccaacaggga aaggcaaaga tgaagtaaag accgaaaatc ttgacttaga agagatggta
    22681 ttaaagctag ctgatgaagg tgtaagatat ttaaaagtat caataactgt atcttatgat
    22741 tcttcatata aagatataga aaaaaatacg cctttaatta gggataatat tataaattgt
    22801 tttatggcaa aaagggcaga tgattttact gctgataatt taaagaacat aaaagaagaa
    22861 attaaaaata cattaaatat acaactaggt gaaaatgtaa ttcaaaatat atatttcaca
    22921 aatatagtag tgcaataaaa ggatggtaag acatgatact ttatttacta aaaatattaa
    22981 tatgtatccc aataatttta atattgatag tcttatcttt aaaaattagc caaggaactt
    23041 tattaaaaaa atataatgat aaatatataa aaatactaga tattgtaaat ataagtaaaa
    23101 acaatagcat aataatttta aaaataggtg aagaaggatg cattatggca tcttcttcct
    23161 ctagcatgaa taaaataaaa gatttgacca aagaagaaat attaagaata gaaaacgaca
    23221 ttaaagaaag aaattttaaa attcaagatt ttaaaatgga aagaattaaa aagaaatatg
    23281 atttcagtag atatattgga aaatttacaa aaaaggaaga taagtaaatg gaactattat
    23341 caaaattacc tttggctgat gtaccatata caagttcaat gcaattgttt ttatttctaa
    23401 ctgcattaat gtttttacca tttgtaatgt ttatgatgac aagctttgtc agaatagtta
    23461 taagtttatc atttttgaaa tcagcattag gagcacagca agcaattcca agccaaattt
    23521 tggtagggct tgcaatagta ttgacaatat ttataatgag accagtatta aatgagataa
    23581 acgaaaaggc attacaacca tatatgaaag aagaagttac tatggaagaa gctatgaagg
    23641 aagcagaggg acctattaaa gaatttttat taaagcaaac cagacagaca gatttagatt
    23701 tatttgtaga acaggctggt ttgaaagaaa agaaattaac tcgtgaaaat ataccactat
    23761 ctgttgtagt tccagcattt gctataagtg aactaaaaac tgcttttcaa ataggttttt
    23821 taatatatat accatttttg ataatagacc ttgttgtggc aagtgttttg atgtctatgg
    23881 gtatgtttat gttgcctcca gtcatgatat cattaccgtt taagttatta ttatttgtaa
    23941 tggtggatgg atggaacttg atagtaaaaa cattgatatt gggatttgga taggagaaaa
    24001 atagtatgag tgaatttatg ataatgagtg ttcttaaaaa tggcatgttt acaggagcta
    24061 tgattgcagc tccagtactc attgtttctc ttgtagttgg gcttttaata agtgtgtttc
    24121 aagctacaac tcaaatccaa gagcaaactt tgacttttgt accaaaacta atagcaattc
    24181 ttttagtagt agctattggt ggaagctgga tgttacatac tcttattagc tttacttcgg
    24241 agatattcaa tatgatgtct cttataacta aataagtagg atatatatga tttttgtatt
    24301 tattagaata gtggcattct ttggaacact aaggatacta tttccagctg gtacacctgc
    24361 tttatttaag tcgctctttg caatcattat atcaatattg atttcaagta ctatgcaaat
    24421 tgaatatgca acaaatatag acaacattgt attgtttacc ttatatggta taaatgagac
    24481 cataactggc attatgcttg gatatataac caatctatgt ttttatagta tcagaatggc
    24541 aggttctatg atggatcaac agatgggatt atcaatgatt aatatgtttg acccaaattc
    24601 aatgactcag acaactttaa ttgataatct tatgaactgg acagcacgta tgatattttt
    24661 tagcatggat ggtcatcatg ttttaattag aggcataagg tatagttttg agttaattcc
    24721 aatagggaaa tcattcgtag ataataatat agattatata ataaacatat ttgtgcagtg
    24781 ctttttaact ggattcaaaa tagcaatacc tattgtactt tgtctgctta tggcagattt
    24841 tatattaggg cttatatcaa gaagtgtacc tcaactaaat gtaatgataa ttggtatgcc
    24901 attaaaaata ctggttggta ttgcgttatt tatcatatca atacctttaa ttgtcaatca
    24961 aataagtcat ttattaagtc aaataccaaa gatgtatgag ggaactttcg cactagctcc
    25021 tatgtttttt atgggttcaa cagataaaac agaagaggct actccaaaga aaaagggaga
    25081 acaaagaaaa aaaggtaata tagcaaagag taaagaatta cccgttgcaa tgactctact
    25141 tgcattcact ttattagtac ctacattatt ttcatatgta gtggatacac ttaaatcttc
    25201 tcttaattat tttttgagtc tagattttta tatgaatata aattattcaa gtatagagaa
    25261 attagttata gcaggattaa tggatttttt taagatattt ttaccaatag ctattccatt
    25321 cttagtttta ggtatagtcg caaacctatt tcaagtggga atactgttta ctggtgaaac
    25381 tttaaaaccc aatttatcaa aattaaatcc agttagtgga tttaaaaaca tgttttctat
    25441 gagaagtttg tcaaccttaa taaaggatac agcgattata tctattttgg cttacatagg
    25501 atatacattc ttccaagata attatttaga tatattaaag ctaggtaaca tatacctgcc
    25561 aactttaatg tacacagtta aagatttagt atattcaata ttaagtaaga tttgtgtagc
    25621 tatgatagtt atagcagttg cagattatgt ttatcaaaga tacagtcata aaaagcaact
    25681 tagaatgaca aaacaagaag ttaaagatga atataaaaac tcagaaggag acccagaaat
    25741 aaaagctaag ataaaacaaa aacaaagaca aatttcgtct caaagaacca tgcaagcagt
    25801 accaagtgct actgtaatag taaccaatcc cacccactta tctatagcag tgagatatga
    25861 aaaagggaaa gaccaagctc cagtagtggt ggcaaaaggt gcagattatt tagcttttaa
    25921 aataagggaa attgcaaagg gaaatgacat acctataata gaaaataaac ctattgctag
    25981 gttgttatat aagcaagtag aaatagacca agaaattcca gaggacatgt atcaagcttt
    26041 tgcagagata ttagtagcag tatataaaat caaaaataga tacaaagtcc tcaaaaggta
    26101 ggataagtaa tgaataagtt taatttaaag gaaaatatgg acgtaatagt ggcatttggt
    26161 gttgtaggga ttgttcttat gatgatagtg ccgttaccta catttatatt agatatttta
    26221 ctggcaatta acataagttt atctgtgcta attttactaa tgacattatt tacaacaaat
    26281 gtacttgatt tatctatatt tccaacagtg ttacttgtga caacactatt tagattggga
    26341 ttaaatcttt catcaactag attgatttta acacagggat atgcaggtga agttatagag
    26401 gcatttggaa gttttgttgt aggtggaaat tacgtagtag gaataattat attcttaatt
    26461 atattaattg ttcaatttgt tgttataacc aatggttctg gaagagtatc tgaagtaaca
    26521 gctagattta cgttagatgc catgccaggt aagcagatga gtatagatgc agatttaaat
    26581 gcaggagtta tagatgacaa aactgctaga aaaagaagaa aagagcttca acaagaagca
    26641 gatttttatg gctctatgga tggagcaaat aagtttgtaa agggagatgc catagcaggt
    26701 cttatagtaa cagcaataaa cataataggt ggaattgtaa taggagctgt aatgctgaat
    26761 atgactctta tgggtgcagc acagacatat gtaagactta ctatagggga tggtcttgta
    26821 agccagatac ctgctctatt aatatctaca tcagcgggta taatcgtaac aagagcttct
    26881 actgatgaaa actttggtgg attagtaagt aaacagttaa cggcaatacc aaaagcagta
    26941 atgatgactg gttgtgttct aatcgtactt ggtatgatgc ctggattacc taaaccagca
    27001 tttttttcac taggtatagg tgctatagca gcaggttact ttttaggaag agaaaagaaa
    27061 gatactttag aagacttgga gtttgaaaaa cctgatatgg taaatccaga taaaaatgaa
    27121 gtagaagatg taacaaactt aataaatgta gaatcaatag aagtagaaat tggatatggt
    27181 ttaatatctt tagctgatga atcagcagga ggagatttac ttcagagaat agcatcaata
    27241 agaagacaat gtgcaataga catgggtata gtcgtgcaac ccataagaat aagagataat
    27301 ttacagttaa atccaaacca atacaatata aaaataaagg gaaatgtagt tggtgggtat
    27361 gagataatgc ctaatatggt tttatgtatg gaccctatga atcaaggatt tagtatattt
    27421 ggaataaaga caattgagcc tacttttgga atagaggcac tttggataga aagtgacaag
    27481 gtagaagatg cagagataaa gggttataca gttgtagatt catccacaat acttataact
    27541 cacttactag atataataaa atcaaatgca cacgaattac tgggaagaca agaagttaag
    27601 actattatag atgcagcaag agaaaattat agtgctgtag tagatgaatt gataccagat
    27661 ttaatgacac ttggtgaaat tcaaaaagta cttaaaaatc tgttaagaga aaaagtaaat
    27721 ataaaggata gggttacaat acttgaaacg cttgcagacc agtctagaaa tacaaaagat
    27781 atagagcttc ttactgaata tgtaagaata gccttggcta gaagtatttg taataattta
    27841 gttgatgatg ataagacaat tgtagtggct acattgtctc cagaaactga agagttggta
    27901 tctaataatt tacaaaggtc tgtaaatgga acttatccag cagttgaccc agaaaataca
    27961 aataaaatat ttgagagtat acaagaagta atgaacaatg tatatttcaa tcataatata
    28021 ccagtaatag tagtatcacc aaagataaga gctccattta gaaaattagt tgaaattgta
    28081 tatcctaatt tgactgtatt atcattaaat gaaataccta acgatattaa aatcaaagca
    28141 gaaggtgtgg tgagcatata tgatgacgaa aaagtataca gctaacacaa ttcaggatgc
    28201 tatgaatcta gccaaaatgg aattgggtga caatattaca ttaatagata aaaaagaagt
    28261 aagaaagtct ggaatacaag gtatcttttc aaagaaagat attgaactaa ctattggatg
    28321 ggaaaagaga gaaaacgtag aacaaaaaga tttaaaacga gagatagaac aattaaagtt
    28381 aattattaac aatatggggt ttgataataa gaatgataat gatatagata aaatatgtaa
    28441 aaatctacta agtttagaat taaatgaaga aattgtagaa tctataagaa ttgatttgca
    28501 agaaatgaag ttcaatggaa ttgatacaag caaaaattta gtagaaattt taaagaaaaa
    28561 gattaaaata gaaaatcaag caataaatgg taaaattgct ctagtcggac caccaggagt
    28621 aggtaaaacg actacaatag caaagttagc cgcaaaatta gtttttgaag aaaataaaaa
    28681 agtaggagta ataactatag atacatacag gattggtgct gtagaacagt tgaagatata
    28741 tactgatata atgaatattc cttttaaagg ggtaataagt cccgatgaaa tggaattggc
    28801 acttgatgag atgaaagatt gtgatgttgt attaatagat acaacaggta gaggctataa
    28861 aaactctatg caaatactag aaatcaaaaa tcttatagat aaagctgaaa ctgataatat
    28921 acatttagtt gtgaattgta caacaagaga aagtgataca aaagcaatta tagattcata
    28981 tagaaatgtg aattttaaaa gcttaataat tacaaagcta gatgaaacaa taacatatgg
    29041 ctcaatattt aacataatga attatgcaca aaaaccaata tcatatatta caacaggaca
    29101 aaatgttcct gatgatatta taaaacctaa tgaagataag attataaggt tactattagg
    29161 agttgaaagt atatgataga ccaagctcaa attttgagaa aaatggcaat ggaaaaaaga
    29221 ggacttgatg agtttatagt agagaatgag aatgtaccta agataattac aatagcttct
    29281 ggtaaaggcg gtgttggaaa gagtaatctc gctacaaatt tgtctatatg cttgaccaaa
    29341 cttaataaaa aagttcttat attagatgca gacataggaa tgtctaatat agatataata
    29401 atgggggtaa atgtaaaagg gactataatt gatgtaatta atggcgaaaa aaatatagaa
    29461 gatataattt cacagactaa gtatggtgta aatataatat ctggaggttc tgcccttaat
    29521 catatagaag actttacaga agctcaaaga aataagttta tacatagcat agaacagatt
    29581 cacgatgtgg attttataat aattgataca ggagctggta tgagcaaaag cctattgtca
    29641 tttatatact gtagtactga atttttctta ataacaactc cagaaccgac atcattgact
    29701 gacgcatata gtttattaaa agctataagt aattttggca taaagagaag tgcaaatata
    29761 ataatcaata gggtaattga tttagaagaa gcaaaatcta catacaaaag aataaaaatg
    29821 gttgtggaca aatttctaaa ccttaatcta gaattgtatg gatatataat agatgacaaa
    29881 aaagtaagtt tatgtgtaaa aaaacagata ccattcattt tagaatatcc tacaagtatt
    29941 gcatcaaaat gtattatagc catagcaaag agaatggtaa gtcagtcaag agaagatatt
    30001 gttgataata aaagttttct aagaaagatg tttagtatat ttaaaagcta aggaggcgta
    30061 gttaatgaat agagaagaat taataaaaga gaatatgcct cttgtaaaga gtatagcaag
    30121 aaaatttttt ataccaggga aaggttttga atatcaagac ttagtaaata gtggagtcat
    30181 tggattgatt gatgcaataa ataaatttga tgctgaaaag ggggcaaagt tttctagtta
    30241 ctcttatata aaaataaaat ctgctatatt agatgaaatt agaaatcaaa gtccaatttc
    30301 taaacataat ctaactaaag taaataagta taatagagtg gtagaaaagt tgcaatctaa
    30361 attattaaga gagccaactt catatgaaat tgcaaaagaa ttaaatgttt cagaaaaaga
    30421 acttcatgat atcgaaagta atatagacat gttaaatatt gtatcattaa actatgtaat
    30481 ctttgaagat acaaatgaaa ctgtacaaga tgtaataagt gatagagaag aggaagctcc
    30541 agaaaatata atagaagaag aagaaaagtt agagattcta tctaaagcaa ttagtaattt
    30601 aaatgaaaga gaaaaattaa tactttcact gtactattat gaagatttaa atctaaagga
    30661 gatagggaaa gtgctaggtg tttcagaatc aagagtttca caactccaca gaaaatcaat
    30721 aagaaactta agaaacaaga taaaagaact taaatattct atatagatgg tgattgataa
    30781 gatgatacaa attatattaa tagtagtgtc tattttaata atagtaacta tagttataaa
    30841 tttacataag gaatcaaaaa ataaagataa cagatacttt gataaaatat ttgagcaaga
    30901 atatgaagca aatgatagat taataattgc agaaaaaaga agattctttt tagacaaagt
    30961 acttgctgaa ataagaagta ataatcatat aaaagtttat gaaaattata acctaataaa
    31021 ggctaatgaa gaaagaaagg aaaacatatt tatagaatca aaaaaggaag taagacaaga
    31081 aaatgagtta tctcttaaag tgaaacatct tataagttca ggttatgatg atgtggaaat
    31141 atgtaagatg ctagatattg gaaaggggga actgtcatta ataaggagtt gttacaaaat
    31201 ataaggatat tattatgtga taaaaaagta ttcttaggaa taggtattgg tatattaata
    31261 tcaactttat gcataatgct ttttagtaat aatgatatga gtaaggcaga gatagaaaaa
    31321 aaggccagag cgttaggcat gaaatataca agtgagatga aagttttaga atagatgata
    31381 ctgtttagac atttacaaaa aaggaggtca taatgtgata agaggattat atacagcagt
    31441 ttctgctatg ataactaatc aacaaaagca aaatgttgtt gtcaataacc tagctaatat
    31501 ggatacaaat ggatataaaa gtaaacaact tttgactaaa agttttgatg aattaaagtt
    31561 ggagggttac aataactacg caaatggaac taaacaaaag cagattatag gcgatataag
    31621 tccaggtatt tctatggatg aaactatcac aaattttaat caaggaccta taaaaactac
    31681 tgataataaa atggatgtag ctattcaagg taagggattt tttctagtta gtgatgcagc
    31741 tggaaatcaa ttttacacta gaaatgggaa cttcagaaca gacaatcaag gtgaccttat
    31801 aacaagtgaa gggtattatg tacttgggac gaatactgct actggtgctg taggtcatat
    31861 aaatgtagga aatcaaaaat ttgaagtggc aaaagataat acaatatcac tagatggaaa
    31921 tgctatgtat aaattcaata ttgtagattt taatgactac aataacttaa aaaaagaagg
    31981 agataacttg tattctggag gaggagctat gaaagtaaat aatgcactta caatacaaag
    32041 tgcgatagag tcttctaatg tagatatggt atctgaaatt aataatatga tgaccatatc
    32101 aagagaatat gaggcaaatc aaaagataat tcaggctatg gattctaaat tagcaaaaat
    32161 agcaagtgag attggttcag taagataggt gataggaggt gaatttttat gtacacaatg
    32221 ttttatacta gtaaaacagc tttatcagca aatcaaagta aactagatat aatatctaac
    32281 aatatagcaa attctgattc aacaggatat aaaaaaataa atatggaata ttctgattta
    32341 gtggatgaag tactaaatag accatcatat cctacaaatg gcaatgatat atcgactgga
    32401 acaggagcta aggcaacagc tcccatgaga atttatgaac aaggtgcatt ggcccctaca
    32461 gatagtaaga gtaatttggc gatagatgga gaaggattct ttagagtgat aagaaaagat
    32521 ggtacatatg catatacaag aaatggtgga tttaatatag atggtttagg taagattgta
    32581 gatgataatg gaaatatatt agatgttcaa tttgatgcag gatataacta taataatacg
    32641 aatatagata gcacaaactt aacagtttca agaaatggcg agttattcgt agataataaa
    32701 aaaataggaa aaatcaattt atatcaacct ataggaactc aaaattttat atctgaagga
    32761 gatagtttat tcgtcgcaac tgatgcagca cagattaaac aagttgagaa agtagatata
    32821 gttcaaggct atagagagag gtcaaatgtt tctttacaag aagaatttgt tgacttaata
    32881 gccactcaaa gagcatttca aatgaactca aagggaataa agacagcaga tgaaatgtgg
    32941 caaatagcta ataatttaag agctaaataa ttatgtaagg tgaggaatat tcatgaatga
    33001 tatgaaagag tacgattttg gcaaacctca gagttattca agagaaaatt taacttgttt
    33061 aaatttttta ttagctgaat tttgtaaaaa atataaaaac tatataattt atgaacttaa
    33121 gtgtaattct agtatgattg ttgatagtat agaccaagta aattatcaga cttttttaga
    33181 tagagtaact tcttcatgtg tagtagtaca aaatgcaatg gaacctgtta tgaaaaactt
    33241 ttcttttaga atagatagaa gtgtagctga tatgtggatt gatataactt ctggaggaac
    33301 tggagttata aaggataatg atagagagct tacagaactt gataaaaaag tgcttttaca
    33361 tcttatggat gatttagtaa gaaatatgca cttatttgaa ggttttgaaa atatacaagt
    33421 attggataca catacacata ttaatttacc acaattatgt tctccaactg caccagtatg
    33481 cgttgtagat ttaaaagtgc taaatgataa tgagtatatt ggaagtgttt ctctttgttt
    33541 tccgtataat ggtttagagg ctctattaga aagtatatct gttatagaat tttttgataa
    33601 tgatgtagga gatgattcag aggaatttac caaaaaaatc tataatagtg tatctaatat
    33661 agaattatca gttattgcag agataggaaa agtatcaatt aatgttgaag acttacttaa
    33721 actagaagtt ggagatgtaa tagtaacaaa taaaaaaata aatgattata tagatatatt
    33781 tgtagaaaat tcaaaatcat atacagcaac accaggattt atatcaagca aaaaaggtgt
    33841 aaaaataaat gatgctgttg gaaaagaggt gtagttatgg acaatagtaa tcttatttca
    33901 gatgaagaag caaagatgtt attaaatgat aaagttgaaa gtgaagttga tggggatgat
    33961 gagttagcct tagatgtctc tgaagttaat cgaaataata tacaaaggat acttgacctt
    34021 aagttagaat tatctgtagt tatagggaga actaagatga ctctaagaga tattttaaat
    34081 ttacataaag gttctcttat tgaactgaat actttagcag accaagatgt ggaaatacta
    34141 atagataata aggttttggc ttatggaaaa gtagtggtgg tagatttaaa ttttggtgta
    34201 aagataacaa gtatagtaag tgaagaagac atggtaaaat ctcttgttta gagataacat
    34261 caattaatac ttaaggcatg aggtggtact tattttaaac ttaaatgata taaaaaaaga
    34321 tatactaaaa ctcaataaaa aagactcatg ttctctatat aatgaatcta ttaataatat
    34381 aaataataat tgtatatcaa ctggattaaa atttttaaaa caagcaagag atttaaaccc
    34441 agatgatgtt gatatattaa atctcacagg tcttgtaaac ttactaaaat gtaattttga
    34501 tgatgctgta gaaagtttct ataagagttc actttgtgaa aaaactgctc tgtgtgaaaa
    34561 gtatataaac atgcttacat ctgaggagtt tttaattttc tttgaaaagt acaatcaact
    34621 tataaggtta ataaataaag gaatgtataa agaagctata caagggttta aacttataac
    34681 tgaccaatac tgtgatttaa tagaacctta tgagttgctt gccttactgt atgacaagga
    34741 agaagaatat ataaaatttg atgaatgttt agaagtattg aagattattg ataaggaaaa
    34801 ttatttatta aatcaagaaa ataacacatt agatatgtat aatagtgcta atatacaaac
    34861 taaaagtaat gtaaaagtag atgattataa gaatgagttt ttacataaaa gtagtagtaa
    34921 ccataaaatt aatgattata ataatgtaac taaagaaaaa agttcaagca aaaaccagaa
    34981 aagttcagat tctaataaga aaaaaagtat taaaaaaaat aagtacctat atatagccat
    35041 aatattagga atattattag taggacaaag tatatatagt tcttataaga tgaataattt
    35101 aactgataaa atatctaaaa ataatgaaac taaaaatgaa gagtcaatag attctaactt
    35161 gactaagaaa gaaaataata tagaagaaaa tgaagatatg agtaaggctt ctaatataaa
    35221 taagagtaat aaaaacatta ataaagaggc tgatgtaaaa tctaaaaaaa atactgtaga
    35281 aaatgaagat gataaagatt ctgacttatt tacagaggat gaattgatgt ctaaggcgaa
    35341 tactttaaaa tcagaaaaaa aaactaaatc ttctatacaa ctatataaga aagttgcaga
    35401 tataggtgaa aataaaggga atacatctga agcaacttat caagttgcta ttctcagtga
    35461 aaagttaaaa gactataaaa ctgctgaaga atattataaa atgtatgtag aaaattactc
    35521 agaaaaagat gcttattttg atgaatctta ttataattta gggatgatgt actataacaa
    35581 tggtgattta aaaaattcta aattgacatt aaagaagtta gttaataagg taccaaatag
    35641 tatgtataat aattcaaaag taaaagaaat tttaaaagaa gaatagttaa attagataag
    35701 aaaaataaat tattttattt taaaataata tgattataat atattgacat accattttaa
    35761 aaaatttaaa atattaatta taaaatgagg agggataaaa aatggaattt gaaaaaggga
    35821 gtatttcaat tcatacagaa aatatttttc ctataattaa aaaatggtta tactctgata
    35881 aagacatatt tataagagag cttataagta atggatgtga tgcagtaagc aaacacaaaa
    35941 gacttgtatc actaggagaa atatcagaaa ataagtcatc agattataaa attacagtat
    36001 ctgtaaataa aggagaaggt acacttaaat ttattgacaa tggaattggt atgactgaag
    36061 aagagataaa aaaatacata aatcaagttg ctttttcagg tgctgaagat ttctttaata
    36121 aatataagga taagatggaa gagtcaaatg acataatagg tcattttgga ttaggatttt
    36181 attctgcatt tatggtgtct aaaaaggtac aaatagatac actttcatat acagaaggag
    36241 cgactcctgt aagatggata tctgaaggtg gtacagaata cgaaatatct gaatcagatg
    36301 ctagaaatga tagaggaact actataacac tatttattga tgatgatagt aaagaatttt
    36361 tagatgaatt tactgtaaga ggaataataa ataaatactg ttctttctta ccagtagaaa
    36421 tatatttaga agatgttgaa agattagaaa gagaagctaa agaagcagaa gaaaaagcta
    36481 aaaaacaaaa ggaagatgga aaagaagaga ttgtagatgc aaaagtaatt gagcctttaa
    36541 acgacactaa tcctctatgg ttaaaatctc caaaagactg tactgatgag gagtataaag
    36601 agttttatag aaaggtattc aatgtatttg atgaaccttt attttggata catttaaatg
    36661 tagattatcc tttcaattta aagggtatac tttacttccc taaattaaaa aatgagtttg
    36721 aactgactga aggaaaagta aaattataca acaatcaagt gtttgtggct gacaatataa
    36781 aagaagttat accagaattt ttacttttat taaaaggtgt tatagactgc ccagacttac
    36841 cattaaatgt atcaagaagt ttcttacaaa atgacaggga tgtaagcaaa atttctaaac
    36901 atataattaa aaaagtagct gataagttaa aatctctttg taaaaatgaa agagaagaat
    36961 acaataagtt ttgggatgat atacaaatat ttattaaata tggttgctta aaagatgaaa
    37021 gtttctatga gaaggtaaaa gaatgtatat tattcaaaac tattgatgat gagtatataa
    37081 caatacaaga ttatttagaa aaatgtaagg acaaacatga aaataaggta ttttatgtaa
    37141 gtgataagga acagcaatct caatacatta aactatttaa agaatacgac ttaagtgctg
    37201 ttgttttaaa ctcatcaata gatactcatt ttatttcatt tatggaatat aaagagaatg
    37261 gagttaagtt taatagaata gatgcagacc tttctgatgt attaaaagat aaaaatgaaa
    37321 ataaagatag tgaagaaaac aaagaagaga tagctaagat agaaggtttg tttaaagaag
    37381 ctgttggaga aagagtgaag aattattctg tagaaggatt gaaaaatgaa gacactccag
    37441 ctatggtatt agtatcagaa caatctatta gaatggcaga aatgcagtca agatttgctg
    37501 gaatggattt aggaatgaat tttgaggaag aaaaaactct tgtaataaat gagaacagtc
    37561 caataatcaa aaaattggtt tctcttaaag atgatgagga aaagaaagat aagattacgc
    37621 ttatttgtaa ccagatagca gatttagctt tactttctaa taaagaatta aagccagatg
    37681 agttagattc atttgtacaa agaagtaata agcttatgag tcttttgata gaattataag
    37741 attgaatttg aatacattga agcaaattta taaaaagtat tcaatatcag tgatgagaaa
    37801 gaagtgtctc aaaatgaact tttcagttca tgaggcgctt tttttatgtg aaattaaatt
    37861 tattttaatc atttcaacaa tgaaaaatat atttatctct attttgagac agaccgttct
    37921 ttattttttt atctttggtt aatttttaaa tatttaagta ggttctaata aaaaactcta
    37981 tttagttaaa tatagttaac ttgatatata ataagataat acatataatt aagtaaaaga
    38041 ttgtttttta gtttatgttt aaatacataa ttacactttg ttaaaaacat aaaattatta
    38101 ctaaagtaga aataggaggt tttagcatga attttaatta taatttgcca gtaaatatct
    38161 tatttgggag aggcagaatt aatgatgtgg gaaaagaagt atctaaatat ggcaaaaaag
    38221 tattgatagt aacaggtaaa aatagtacaa agaaaactgg attacttgat aaaacaatag
    38281 atttactaaa agactctaaa atagaatatg aagtttttga tagagtagaa caaaatcctc
    38341 ttataactac tatatattca ggcgtagaaa ttataaaatc aactggatgt gattgtgttt
    38401 tagggcttgg tggtggaagc attatggatg ctgccaagag tatagctttt tcttataaaa
    38461 acccaggaga tttaaatgat tatatatttg gaattagaca aggtgaagaa gctttaccta
    38521 taatcttagt tccaacaaca tcaggtacag gtagtgaagg aaattgtttt gcagtactta
    38581 caaattcaga aactaaagat aaaaaatcta taaagaagaa tagtatgtat gcaaaagcat
    38641 caataattga cccagaactt atggttacaa tgccaaagca tataattgca tcagttggat
    38701 ttgatgcact tgcccataat atggaagcat atctttctaa tggaagaaac cctcttgcag
    38761 atgtacaagc tatttatggg attgaactta tatcagaaaa cctaataaag gtatataatg
    38821 atgttaatga tttagaagca tgggaaaaaa taacacttgc aagtactatt ggaggaatga
    38881 cgattggtac agcgggtaca gccttgccac atggaatgga gcatcctgta agtggtctta
    38941 gagatgtaat gcatggaaaa ggacttgcag cgttgactcc tactattata gaaagttcat
    39001 gggatattga tattgaaaga tttggtaata tttcaaaggt attaggagga acttgtgcag
    39061 aagattgttc agatgcaatt agaaatttcc ttaaaaaaat agatttagat ataaaactaa
    39121 gtgatttagg tattgaaaaa tcagatatag attggttaac tgataattgt atgaaaatat
    39181 cagttgcaaa tattaagaga catccaaagt attttaataa ggaacaaata aaggaaatgt
    39241 accataagtc tttataattt gactttacat atttagtttt atataaaaat tataaattaa
    39301 taataaaaaa ttataagtaa ttaatagtaa aaaaattgtg tatagaagtt atatatataa
    39361 taaacttgtc ttaagctttt attttacttt ctaataaata aaggagttta aaaaagggaa
    39421 gattaattaa gtaatatttt ataaaattct tcgatttaaa aactttaagt agaggtgaaa
    39481 taaatgttta atcccaaaag aataatattc gaaaaaggaa ctttagacac tgatgtgggc
    39541 aaaaaaatat acaataaatt tagagataat aaagatgtag agataattaa tatatcatca
    39601 aataggatta aagaacacat tccaggtgaa aatctatatg aacaatatag aagtgggaag
    39661 cagactatgg tagttgggat tagaaaaagt ctgaagtttc aaacatgtaa accttctgct
    39721 aattatcaac taccacttgt cagtggatgt atgggaagat gtgagtattg ttatttgaac
    39781 acacaattag gtgataaacc atttataagg gtgtttgtaa atgtagatga aatacttgaa
    39841 cagacaaaaa aatatattga agatagaaag ccacaggtaa ccatatttga aggggcagct
    39901 acatcagacc cagttccagt agagccatat actaattctt tgaagaaaac aatagagttt
    39961 tttggaagag aggaaaaagg aagatttaga tttgttacta aatacagtga tgtggatagc
    40021 ttattggatg ctgaacataa taatcataca gaaatacgat ttagtatcaa tactcctaga
    40081 attataaatg aacatgagca ttatacagcc tctgcaaata aaagaataga gtcagctgta
    40141 aaacttgcta gggctggtta tcagattggt ttcataatag cacctgtgtt tttatatgaa
    40201 ggttggaaag aagagtataa aaatctgatt ataagtataa aagaagcttt accaaaggat
    40261 tttgataaaa aaattatatt tgaagttata tctcatagat atacaactaa agcaaaaaat
    40321 agaatactag agatttatcc agatacacta ttgccaatgg aaaatgagga tagaaagttt
    40381 aaatacggtc aatttggata tggtaagtac gtgtatgata aagaacaatt agatgatatg
    40441 aaagagttct ttcttaagga aataaatgga gtatttaaaa atagtttaat aaaatatata
    40501 atatagcaaa tcttacctaa agatttgcta tattttattt ttatatattt tatgtttata
    40561 ataaagcaat gagatttagc actatcatat aagaaaatta aaaagggcac tatctatcaa
    40621 gaacattttt tctagttttg atactataaa gggtaagagt aacaaaaata tcaggaggag
    40681 ttatagtatg catgcatggg agtctattca aattacattg gatttaatag aagcaaattt
    40741 atcagaagaa atatcaatta atgaattagc aaataaagca aatttatcac cattttacta
    40801 tcaaagacta tttaaaagat tggtaaataa gactgtaatg gagtatataa agcttcgtcg
    40861 tcttgcaaga gcttctgaat acttagcaga acataagaat cgtatacttg atgtagcttt
    40921 aaattttgga tttgctagtc atgagacatt tacacgttcc tttaaaaaag catatggatt
    40981 aactccagaa aaatatagag caaatccagt aaaactcaat cactttatta agccagaatt
    41041 aattttaaat tatgctatgg ttgatgaagg tgttccactg attgcagatg atatagtgat
    41101 tgaagtcaat cgtaagacat tgagtaatcc aaggacattt gttggaatag aaatagaagt
    41161 acctatttgc cagttggtgg gtggtgaaac aactggaatt gcaatagtag aggaattatg
    41221 gatgaaactt ggaagtcaaa gacataatat accaaatcaa attccaggtg gaaatgaatt
    41281 tgctgctctt tatatgggaa atgcaaaaga aggaaattgt atgtatatgg caggtgtaga
    41341 ggtagagtca ggtacatcag tagaaggata taatactttt gagttgccag ctaaagaata
    41401 tttagtatgt ggatttgagg cagaaaactt taatgagtta gttaattcag ctgtttttaa
    41461 agcagataaa tttatggaaa gatggatgaa gaaacataat ttaacaacta ctgattttgc
    41521 aattgagatg tattacccca ctacacctga agcagcatat ctggaacatt ggattgttcc
    41581 ggttcctatt gaacaataga aaggatgatg aatatgaatt ggcaagatgc gttttttcaa
    41641 gatactaaac ctacatttga gcaaattaat gaatttatta ataatccatt atgggacaag
    41701 ttaaataaaa ctttggtttc tacttataat gtagaaccta agcttgaata cagtaaatgt
    41761 tcaatgcaaa gaggttggaa tgtaaaatat aaaaaaagag gtaaatcact atgtacttta
    41821 tatccacaag aaggttattt taaagctttg gttattgtaa gtgaaagcaa tagagtagag
    41881 gttgatttat ttattaatac ttgttgtgat tatatcaaaa aaatatataa tgaggtaaac
    41941 ttttttaatg gaagtaaatg gctaatgatt caagtagata gtttattggt attaaacgat
    42001 atgcttgaac taattaaatt tagagcttaa catatagaac aggatgaata tataagtcat
    42061 cctgttttta tattcaatca gataaattaa tatatagtct ataatatgaa taatttagta
    42121 aatttacttt atctttctac attctttgca actaagtcca catgtgtttt tttcttgcat
    42181 attagtagca ccccaattag caaattgttg taaaataggc acaatacttt tacctttact
    42241 tgttaatgta tactcaactt ttggaggaat ttcattatat tgtttacgat gaacaatacc
    42301 agtagactct aaatctttta atgattgagc tagcatagta tttgtaatgc catccaattt
    42361 tctttttatg ctactatatc taattggatt ttcaactgct aacatacata taataggtaa
    42421 tttccactta ccaccgacaa tgtctaatgc ataacggatt ggacaggtta attcttcaac
    42481 gtttttagtt atattttttt cagtatttat actcatttta attactcctt agttatagta
    42541 gtatcttttt tatactttat cagaaaaaac tgcatacttg ttttttctgt attttatatt
    42601 aaaattatat caaaggataa tccttaatac aagtatttaa aaggggtgta agaattatga
    42661 aagcattaga atttttgaac aaggcaggag tttattattt agcaacagtt gatgagaaca
    42721 atcaagcaca tgtgcgtcct ttaggatttg ttatggaata caatggtaaa cttacatttt
    42781 gcacatctaa tcaaaaggat atgtttaagc aacttatttt taatccaaag gttgaacttt
    42841 gttgtataga ccaaaacctt aatactctac gtattttagg tgaagcagta tttgttacat
    42901 caaaagaaac acaagctaag gcacttgaaa taatgccaag tttaggtcag atgtactctg
    42961 tgggagatgg taagtttgaa atattctcta ttgataaggc aaaagttagt tgttgttcaa
    43021 tgagtggtaa aaagattgat gtagagcttt aattataatt gaatcgacat ccatagtgaa
    43081 aattaagaaa acgttattac tgaaatattg atagatttaa tttaagtgca ataaaaagat
    43141 ttttaaataa tgaatatgta aaaattttta gttattagat gtgtatttca ataatgatat
    43201 actattttga ataattacat actagaaata agataattaa aatttacttt ataatgggat
    43261 agatttcttt taaaaaatat tttaactata attgattttt atttaatctg aaaaaaatat
    43321 tatgactata aaaaattgct acttattatt tggatatttt tgtatataaa taataagtag
    43381 catgacttta tacattaata atgatagaat ataaattaat agataagata taattttaaa
    43441 aggagtgata taatatgagt aaatatagtt ctttatggga atatgttaag aaaaataata
    43501 gtcaatcgtt taaattaact tttgaagaaa taaaagatat tgctggaata gagatagacc
    43561 attcgttttt gaaatataaa aaagaattaa atgaatatgg atatcaagta ggaaaaatct
    43621 ctttaaaaga gaagactgtt attttcaata aaattgatta agatataaat aattacat
//
LOCUS       DAEPLF010000009        43186 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEPLF010000009 DAEPLF010000000
VERSION     DAEPLF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43186
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.009"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(79..2424)
                     /gene="secA"
                     /locus_tag="KR314_000482"
     CDS             complement(79..2424)
                     /gene="secA"
                     /locus_tag="KR314_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBH0326888.1"
                     /translation="MSVLDAILDKADEQEIKKLNLIVDKIESLEKDMEIKENEQLKEM
                     TNTFRLRLDKGESLDDILPEAFAVVREVSKRVLGMRQYKVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYEFLGLTVGVILSNQD
                     PSIRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEIDSILIDEAR
                     TPLIIAGDGDEDLKLYELANNFVKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNRPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSKMLKK
                     QGIKHQVLNAKQHDKEAEVISKAGKLDAITIATNMAGRGTDISLGAGDKEEEEKVKNL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIKEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
     gene            complement(2660..4936)
                     /gene="slpA"
                     /locus_tag="KR314_000483"
     CDS             complement(2660..4936)
                     /gene="slpA"
                     /locus_tag="KR314_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBH0326889.1"
                     /translation="MNKKNIAIAMSGLTVLASAAPVFAAEDMSKVETGDQGYTVVQSK
                     YKKAVEQLQKGLLDGSITEIKIFFEGTLASTIKVGAELSAEDASKLLFTQVDNKLDNL
                     GDGDYVDFLISSPAEGDKVTTSKLVALKNLTGGTSAIKVATSSIIGEVENAGTLGAKN
                     TAPSSAAVMSMSDVFDTAFTDSTETAVKLTIKDAMKTKKFGLVDGTTYSTGLQFADGK
                     TEKIVKLGDSDTINLAKELIITPASANDQAATIEFAKPTTQSGSPVITKLRILNAKEE
                     TIDIDASSSKTAQDLAKKYVFNKTDLNTLYRVLNGDEADTNRLVEEVSGKYQVVLYPE
                     GKRVTTKSAAKASIADENSPVKLTLKSDKKKDLKDYVDDLRTYNNGYSNAIEVAGEDR
                     IETAIALSQKYYNSDDENAIFRDSVDNVVLVGGNAIVDGLVASPLASEKKAPLLLTSK
                     DKLDSSVKAEIKRVMNIKSTTGINTSKKVYLAGGVNSISKEVENELKDMGLKVTRLAG
                     DDRYETSLKIADEVGLDNDKAFVVGGTGLADAMSIAPVASQLRNANGKMDLADGDATP
                     IVVVDGKAKTINDDVKDFLDDSQVDIIGGENSVSKDVENAIDDATGKSPDRYSGDDRQ
                     ATNAKVIKESSYYQDNLNNDKKVVNFFVAKDGSTKEDQLVDALAAAPVAANFGVTLNS
                     DGKPVDKDGKVLTGSDNDKNKLVSPAPIVLATDSLSSDQSVSISKVLDKDNGENLVQV
                     GKGIATSVINKLKDLLSM"
     gene            complement(5162..6751)
                     /locus_tag="KR314_000484"
     CDS             complement(5162..6751)
                     /locus_tag="KR314_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HBH0326890.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGSPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNIDTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYSLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVINGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            complement(6970..8571)
                     /locus_tag="KR314_000485"
     CDS             complement(6970..8571)
                     /locus_tag="KR314_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBH0326891.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADRQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASKSNGQPPTITVGSTEIYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSYDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKNLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            complement(8938..10818)
                     /locus_tag="KR314_000486"
     CDS             complement(8938..10818)
                     /locus_tag="KR314_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBH0326892.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSVTCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTVDYENINSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKITKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSNSTSNVTVKKLKGADRFETA
                     TKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAVQFIKSNKVSNIYFIGGI
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVVDKIKSLI
                     EK"
     gene            complement(11028..16991)
                     /locus_tag="KR314_000487"
     CDS             complement(11028..16991)
                     /locus_tag="KR314_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HBH0326893.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGN
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNIGTDNIEYTIK
                     KLSASRSEIKNKDENVSYYNIVFNNLPVGKYNIEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDEDGNVQQ
                     IPYENKEYMKNLKDKEQQEEVNNEQEEVNQEEVNNKDENKNQTDNNSNNKDENNDKQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEGDNISKDDNSTTSTYSRAALE
                     TYTDVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSLKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESIDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVYDKTINDFEIRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIDRELKLAQDLYNDRATLTDVTTVNQ
                     EINKSGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLKTGKNVIQLSKLHSLDIECGGSLYIRYPEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNIGNSY
                     EYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKAKFTDSISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDVSTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPEAETRKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDDKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDPSTSEDETGKIPAGS
                     IIITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNAIDVSNKKVITVSNKVKGNIVRDK"
     regulatory      complement(17115..17200)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(17372..18775)
                     /locus_tag="KR314_000488"
     CDS             complement(17372..18775)
                     /locus_tag="KR314_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBH0326894.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILIDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALVASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     DNGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKISDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(19237..21132)
                     /locus_tag="KR314_000489"
     CDS             complement(19237..21132)
                     /locus_tag="KR314_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBH0326895.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKSETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            complement(21758..21901)
                     /gene="scfA"
                     /locus_tag="KR314_000490"
     CDS             complement(21758..21901)
                     /gene="scfA"
                     /locus_tag="KR314_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBH0326896.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(21967..22329)
                     /locus_tag="KR314_000491"
     CDS             complement(21967..22329)
                     /locus_tag="KR314_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBH0326897.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(22387..22680)
                     /gene="yajC"
                     /locus_tag="KR314_000492"
     CDS             complement(22387..22680)
                     /gene="yajC"
                     /locus_tag="KR314_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBH0326898.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(22814..23935)
                     /gene="tgt"
                     /locus_tag="KR314_000493"
     CDS             complement(22814..23935)
                     /gene="tgt"
                     /locus_tag="KR314_000493"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBH0326899.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITSIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPQDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(24014..24499)
                     /locus_tag="KR314_000494"
     CDS             complement(24014..24499)
                     /locus_tag="KR314_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326900.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(24511..25536)
                     /gene="queA"
                     /locus_tag="KR314_000495"
     CDS             complement(24511..25536)
                     /gene="queA"
                     /locus_tag="KR314_000495"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBH0326901.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFLYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGNNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(25854..26873)
                     /gene="ruvB"
                     /locus_tag="KR314_000496"
     CDS             complement(25854..26873)
                     /gene="ruvB"
                     /locus_tag="KR314_000496"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBH0326902.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(26980..27582)
                     /gene="ruvA"
                     /locus_tag="KR314_000497"
     CDS             complement(26980..27582)
                     /gene="ruvA"
                     /locus_tag="KR314_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBH0326903.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASSAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(27575..28084)
                     /gene="ruvC"
                     /locus_tag="KR314_000498"
     CDS             complement(27575..28084)
                     /gene="ruvC"
                     /locus_tag="KR314_000498"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBH0326904.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(28396..29025)
                     /locus_tag="KR314_000499"
     CDS             complement(28396..29025)
                     /locus_tag="KR314_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326905.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKNNGVQTSK
                     VVSASANMDEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMNATIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            29228..29551
                     /locus_tag="KR314_000500"
     CDS             29228..29551
                     /locus_tag="KR314_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBH0326906.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            29819..30673
                     /locus_tag="KR314_000501"
     CDS             29819..30673
                     /locus_tag="KR314_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326907.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(30859..32295)
                     /locus_tag="KR314_000502"
     CDS             complement(30859..32295)
                     /locus_tag="KR314_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBH0326908.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNELLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPVETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTHGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKEMLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(32366..33748)
                     /locus_tag="KR314_000503"
     CDS             complement(32366..33748)
                     /locus_tag="KR314_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH0326909.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAVLIGGWIYGKRFEKYPAYAIDENDREKN
                     LEKGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKRYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(33884..34789)
                     /gene="garR"
                     /locus_tag="KR314_000504"
     CDS             complement(33884..34789)
                     /gene="garR"
                     /locus_tag="KR314_000504"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBH0326910.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            35185..35850
                     /locus_tag="KR314_000505"
     CDS             35185..35850
                     /locus_tag="KR314_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0326911.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDVYKTTLIHMETPKYINLEDFC
                     NNK"
     gene            35958..36506
                     /locus_tag="KR314_000506"
     CDS             35958..36506
                     /locus_tag="KR314_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326912.1"
                     /translation="MIFLNENVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTIEILRKVLFERSEERAIAMRERALENGDELIADNIISTTDVPFLGWVPEFKE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            36768..37076
                     /locus_tag="KR314_000507"
     CDS             36768..37076
                     /locus_tag="KR314_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326913.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(37238..39028)
                     /locus_tag="KR314_000508"
     CDS             complement(37238..39028)
                     /locus_tag="KR314_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0326914.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(39028..40755)
                     /locus_tag="KR314_000509"
     CDS             complement(39028..40755)
                     /locus_tag="KR314_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0326915.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSARVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(41000..41564)
                     /locus_tag="KR314_000510"
                     /pseudo
     CDS             complement(41000..41564)
                     /locus_tag="KR314_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
     gene            41853..42965
                     /locus_tag="KR314_000511"
     CDS             41853..42965
                     /locus_tag="KR314_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBH0326916.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     AEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
BASE COUNT        13317 a         7716 c         4905 g        17248 t
ORIGIN      
        1 ttaattattt aattgtaatt acaatataaa gaatcaaaat attttatttt tcatttaata
       61 aaaattacat gaactttttt agttaaattt atataagtat tgtactgttg cttcccttat
      121 atttttattt aaagcttcaa acatatcata tccttctaaa gcatattctt taaacggatc
      181 tttttgagca taagatttaa gacctatata ctgtcttaac tgttccatag catctatatg
      241 atctatccag tattgatcaa ccacttttaa aagtactgtt ttttctaact ccgcaacttt
      301 atcaatacca atcatcatct ttttcaagtc ataaaccctt tttgaaattt catatgtgct
      361 atcaattatc tcttggacac tttttttatc tacaccgggt attagtaatg tatctgcagg
      421 cataaatgta ctatataaat gtttaaaata tccataataa tctctttttc ttccaattaa
      481 ataagtttct cctgcttctt gtatgatgtc tttaaccatt ttttgaatat cttcttttat
      541 atcttcatca tttaacactt tatttctttc attatatata acctttcttt gctcattaat
      601 agtatcatca tatttaagaa cattttttct tatttcaaaa ttcttacctt ctacaccttt
      661 ttgtgctctt tctatagccc ttgtaagtgc tttactttca atagccgtat tttcctttga
      721 acttgttctc ttcataagtt tctctatagt ttttccacca taaagcttta ttacatcatc
      781 ttcaagactt acaaaaaatc ttgatgtacc tgggtctcct tgacgaccag aacgccctct
      841 aagctgattg tcaattcttc ttgattcgtg tctttctgtt cctataacat aaagaccacc
      901 taaattttta actttttctt cttcttcttt atctcctgca cctagagaaa tatccgtacc
      961 tctacctgcc atatttgtag caatcgttat agcatctaat ttaccagctt tagaaattac
     1021 ctctgcctct ttatcatgtt gtttagcatt taagacttga tgcttaatac cttgcttctt
     1081 aagcatttta gataacttct cagatttttc aacagaaact gttcccacga gtattggctg
     1141 tctagtctta tgtatcctta ttatttcttc tacaacagca ctatactttt cttcttctgt
     1201 tttaaatacc ttatcatgca aatcagctct aataactggt ctattagttg gtatttggac
     1261 aacatttaat ttatagattg actcaaattc cccttcttct gtctttgcag taccagtcat
     1321 accagaaagt ttttcatata gtctgaagaa attttgataa gtcacagtag ccatagtttt
     1381 agattcattc tttatctcaa caccttcttt ggcttctata gcttggtgaa gtccatctgt
     1441 atatcttcta ccatccatta ctcttcctgt aaattcgtca actatcatta cttctccatt
     1501 tgagataaca tagtcaacat ctttttccat aagcttatga cctcttaaag cttgatttat
     1561 atgatgatat aattctatat tctttatatc agtaaggttt gttataccaa aaaatgactc
     1621 agctttgctt ataccacttg ctgttaatgc tatagtttta tcttttctat ccatctcaaa
     1681 gtcttcttct ttaacagttt ttacaaagtt atttgctaac tcataaagtt ttaaatcttc
     1741 atcaccatct cctgcaataa taagtggagt tctagcttca tctattaata ttgagtctat
     1801 ctcatctact atggcaaagt ttagttccct ttgtacttta tgagacaaat ctggcaccat
     1861 attatctctt aaatagtcaa atccaaattc actatttgtt ccataagtta tatcacattt
     1921 atattgttct tttcttatac ttgggtcttg gttagataaa attacaccta cagtcaatcc
     1981 taaaaattca tatactggac gcataagttc tttatcacgt tctgccaaat aatcatttac
     2041 tgtaattaca tgtacacctt ttcctgttaa agcatttaga tatactggag ctacctcaac
     2101 taaagttttc ccctcaccag tcttcatctc agctatcttt ccttgatgaa ttactatacc
     2161 tccaattaac tgaactttat attgacgcat tcctaaaact ctttttgaga cctctctaac
     2221 aacagcaaat gcttctggaa gtatatcatc caaactttca cctttgtcta atctaagtct
     2281 aaaagtattt gtcatttctt ttaattgttc attctctttg atttccatat ctttttctaa
     2341 agattctatt ttatctacta ttaaatttaa ttttttaatt tcttgttcat ccgctttatc
     2401 taagattgca tctaaaactg acatattttt tgacctcctc ttttatcata caattattat
     2461 tataccttaa aattatagaa tagtagacaa ttattttatt gtatagtata ataatcatac
     2521 ttaaataaat tacatcttta tatatttaag tatttctcac ataaaaaaga gactaaattt
     2581 agtctctttt tttgtattcg aaaacataat ataaaaagac ttctcatata agaagccttt
     2641 ttatattatt atatttatat tacatactta ataaatcttt taatttattt ataactgaag
     2701 tagctatacc tttaccaact tgaactaagt tttctccatt atctttatca agaactttac
     2761 ttatagatac actttgatct gaagataaag aatcagtagc taatactata ggtgctggag
     2821 atactaattt atttttatca ttatcagaac cagttaatac tttaccatct ttatctactg
     2881 gcttaccatc agaattaaga gttacaccaa agtttgctgc aactggagct gctgctaaag
     2941 catcaactaa ttgatcttct ttagtagaac catctttagc tacaaagaaa ttaactactt
     3001 ttttatcatt atttaagtta tcttgataat aagaagattc ttttataact tttgcattag
     3061 ttgcttgtct atcatctcca ctatatctat ctggagattt acctgtagca tcatctattg
     3121 cattttcaac atctttagat acactgtttt ctccacctat tatatcaact tgtgaatcat
     3181 ctaagaaatc ttttacatca tcatttatag ttttagcttt tccatctaca actactattg
     3241 gtgtagcatc accatcagct aaatccattt taccattagc atttcttaat tgagatgcaa
     3301 ctggagctat actcatggca tctgctaatc ctgttcctcc aactacaaat gctttatcat
     3361 tatcaagacc tacttcatca gctattttta gagaagtttc atatctatca tctcctgcta
     3421 atcttgtaac tttaagtccc atatctttta attcattttc tacttcttta gatatagaat
     3481 taactccacc agctaaataa actttctttg aagtatttat acctgttgta ctctttatat
     3541 tcataactct ctttatttca gcttttacgc ttgaatctaa tttatctttt gaagttaata
     3601 ataaaggagc tttcttttca gaagctaaag gagaagctac aagtccatca actattgcat
     3661 ttcctccaac caatactaca ttatcaactg aatctctaaa tatagcattt tcatcatcag
     3721 agttataata tttttgactt aatgctattg cagtttctat tctatcttct cctgctactt
     3781 ctatagcatt tgaatatcca ttattatatg ttcttaaatc atccacataa tcttttaagt
     3841 ctttcttctt atctgactta agagttaatt taactggtga attttcatca gcaattgaag
     3901 cctttgcagc actcttagtt gtaactcttt ttccttctgg ataaagaacc acttgatatt
     3961 ttccactaac ttcttctact aatctattag tatctgcttc atccccattt aatactctgt
     4021 aaagagtatt taaatctgtt ttattaaata catatttttt agctaaatct tgtgctgttt
     4081 tactagaact agcatcaata tctattgtct cttcttttgc attcaatatt ctaagtttag
     4141 ttattactgg gcttccagat tgtgttgttg gtttagcaaa ctcaatagtc gcagcttgat
     4201 catttgcact tgcaggtgtt attattaatt ctttggctaa atttatagta tcactatctc
     4261 ctaatttaac aattttttct gtttttccat ctgcaaattg aagacctgtt gaataagtag
     4321 ttccatcaac taaaccaaac tttttagttt tcatagcatc ttttatagta agtttcacag
     4381 cagtttcagt tgaatctgta aaagctgtat caaatacatc tgacatagac ataactgcag
     4441 cactacttgg agctgtattt tttgctccca gagtaccagc attttcgact tcaccaataa
     4501 tactacttgt agctactttt attgcactag ttccacctgt taaatttttt aatgcaacaa
     4561 gtttacttgt agttacttta tctccctctg ctggagagct tattaagaaa tctacataat
     4621 ccccatcacc taaattgtct aatttattat ctacttgtgt aaacaataat ttacttgcat
     4681 cttctgcact aagctcagct cctactttta tagtagatgc taaagttccc tcaaagaaaa
     4741 ttttaatctc tgttatactt ccatctaata accctttttg taattgttca actgctttct
     4801 tatacttgct ctgtactact gtatatcctt gatcaccagt ctcaactttc gacatatctt
     4861 ctgctgcaaa cacaggtgct gcagaagcta atactgttaa tcctgacata gctattgcta
     4921 tattcttctt attcatttct taaattcctc ccaacattat attaaaatta catctcttca
     4981 ttatgtacat agaataatca tccatatatt cctaatattt ctcacatctc tataataatc
     5041 catttttatt ctttttgcaa gtcatttttt tgaaattatt atttttccca agcatatgag
     5101 tttttatacc cttaaactcc aatatttaat aacttaaaaa atttgatttt acttatttaa
     5161 attacttatt taaaaattct tgtgtagcat acacttctcc attaggcaat atatataatc
     5221 caaacccagt atatacaaaa tcactattta acatattttc attatattta tctgactttt
     5281 tccaaactgt aaatattaca tttgctactt ctctagcatc ctttgttgta taagtacttt
     5341 ttgtaacaag tggtacaaaa gctatatttt cttcacttct caagccatat ccagtaaata
     5401 cttcaacaga tttcttacca tttattacat gtgaaaactg acctttatct gccatgtact
     5461 tagaccagga attagaaagt ccttctaagt ttttagaaac ttgtaatggc tctttacctt
     5521 tttcttttct atatgaattt accaaactat acatttcact agaaaccatt cttttaaact
     5581 cactactatt catatcatta gtcttatatt caagtacatt tacttctaca cttattgatg
     5641 tagtctttcc accaatatca gtagctttta tagtaacttt gtatttaccc accttatcag
     5701 tatcaatatt tcctataagc tctggtgtca aatcgttacc attgctatcc tttgctacga
     5761 tattcagttt acttatatca aacttatcac cttgataaat atttaaattt ccaacagtta
     5821 ttgttggtgc acttgcatcc agtgatgatg cacttaaaca ttgttctagt gtcttttcat
     5881 ctattcctcc tagtgctgta atcttatcag cacttctaag tacagactta tcactatttc
     5941 cgtcaactaa tacaataggt gcattttttg ctatagaaga accagcaact gcatctacta
     6001 attgataacc tttagaaaga taaaattctt ttgccgaact ataaaaatgt tttataactt
     6061 ttttattagt ctcaaatcta tcttctccag ctaatctaac tgcattagtt tttttaacta
     6121 aatcattact tattatctcc tctgaaccaa gagcataaca ttgcacacct tcttttttct
     6181 caaatggaac atttttacca ttagttaaaa ttataggtga tccatctctt gatgctactg
     6241 atgacgcact catagcatct gcttcacctg tataaccatt agttataaaa accttatcta
     6301 taggttttat tgaagctatt tcttttgcta taagataact tgtttcaatt ctatcattac
     6361 cacctattct tttaacttct attccttttt gctttaattc attttcaact gattttccta
     6421 ttgaatcttc agttcctatt atataaacct tctttacatc ttttaacatt ttttctgtgt
     6481 ctgatggtat actgtttctt tgtgataata atataggtgc tttaactgtt cctgctaatc
     6541 cacttgcact taatccatca gctagtgtat tatctgtatt tactaatact acattttcat
     6601 atgtttgttt ctgtgcaatt ttggcagctg tctcatatct atctttccct tgtatctttt
     6661 ctatgctcga aagtgcattg atatttggtg ctcctgccat tattaatgat attgtcaatc
     6721 ctattgataa tactctctta tttaccttca tcgaactcct cctactaatt tttttatata
     6781 attcagctaa tcataaatta catcctcagt agtgattccc tcaaaatcta taaactacta
     6841 aaacaaaaaa cacctaaaat accatagcct gtaaagcaat aacaaaacag attacaaaaa
     6901 gtactttaga tgtgattttc attttataat atcagtaaat taacaaaaaa acaagattta
     6961 tggaattttt tacaaactac cttcattgcc atttaaaaac tcctgagtag catgcacttg
     7021 accatcagat aatatataaa gcccaaatcc agtactatag aactcatcac taagcatgtt
     7081 tgcattgtac tctggagact tcttccacac ttcaaatata gcctttgcca agttttttgc
     7141 atcttgtgtc gtttgaacgt tcttagaatc tatataaata tatgctatat tttcctcact
     7201 tctcattcca aactcagaaa atacttgtgg agcatttttt ccatctatat aatgtgcaaa
     7261 tactttttta tccatcatat acttagacca tgcatttgcc ataccttcta gcctactaga
     7321 tactaccaaa ggctctttgc ctttttcttt tctataggaa ttgattaagt tatacatttc
     7381 agtagatacc atttttttaa attctgggct gttgtaatca taggatttat catccaacac
     7441 ttttatttct actctttttc ctgtactttt tccccattcg tctgtagctt ttaatgtcaa
     7501 tatgtatgta cctactctat ttgtatctat aaacccatca acttctattg gtaatacctt
     7561 cccagtgtta tctttagcca ctatgtttaa cttgccagtg tcaaactttt cacccttata
     7621 tatctctgta cttccaactg taattgttgg tggttgtcca tttgactttg aagcatttat
     7681 acactgttgt attaccttct catttatttc acctacagaa gttatattct tagctccttc
     7741 taatacagtt ttatcgcttc catcatttac tagtaccatt ggagagtttt tagtaagtgg
     7801 tgcagctgct attgcatcta ctaattggta tccatctgat acatagaatc catctgcact
     7861 attaaaaaag tagtctatta catttttgtt agtttcaaat cggtcagttc cttctattct
     7921 tactgagttt gtatttttaa ctagagggtt actcattatc tcctctgaac ctatacaata
     7981 tgattgaata ttttttacat caaaaggcac actctttcca tctgtaagta tgattggagc
     8041 tccatctcta gtagctactg atgatacact cattgcgtct gcttctccac tatatgcgtt
     8101 agttaatagt actttatcca ctttttttat agttgctatt tctttagcta ttaaatagct
     8161 tgtttttagt ctatcttctc caccaattct tttgacttct atatttttag aatctagttc
     8221 tttttctact gatttactta tagtatcttc tgtacctatt atatatgctt ttttgatatt
     8281 ttttagacat ctatttgtgt ctgctggtat cttattttgt tgtgtaaata gtattggagc
     8341 ttttgtagct cccgataatc cacttgcact taatccatct gcaagtgatt tttctgtatt
     8401 tactaaaatt actgtttcat aagtctgtct atcagctatc tttgctgctg tttcatatct
     8461 atctttaccg tatatttttt ctactactga tgatgcattt acacttttga agtttactaa
     8521 gattaatgat actgctagac ctaatgataa tatttttttg ttcattttca tatccccctg
     8581 atgattttta tattttatat ttatattgtg attattttct atttattttc tctttatttt
     8641 ccatatatta tagagtatat atgatatgaa tatatctcgt ctatatatta gtgttttatt
     8701 ttattaaatt taaaaaatta tttttaactt ctttttaaac atattcaaaa ttgaatttat
     8761 atttatatat ataaaagata tctgctttat ttaaaattaa acttaatata gctcaaatta
     8821 ttatacattt taacctcaat attattaaat tgttaagata atgaaaataa aataagaatg
     8881 catctaaata tataacttta tatctaaata cattcttatt ttcgatttac ttaatattta
     8941 tttttcaatt aaactcttaa tcttatctac tacagatgag gctattcctc cacctacttc
     9001 aaacaacttt gatgaatttt taccttttaa tgatgattct tgtgatttat ctaacgtatt
     9061 tgtagcaagt ataactggtg aattgttttt agctgctaat ggtccaactg ctaaagcatc
     9121 tataagttta tcttgatttg caactacaac tccatctagt ttagattgag agtagaattt
     9181 atctattact ttggcattcg tctcttgtct agtttgacct gccaccctat tgtttgatat
     9241 atctttgttt gctatttttc caacttgttc aatagccttg ttagatattt tttctattcc
     9301 accaataaag tatatatttg acaccttatt tgatttaata aattgtacag cttcattatc
     9361 tacaccatct ttttgtgtaa gtactattgg agttttttct ttaccggcca gtgatgctat
     9421 tgataatgaa tctgcttctc catttcctgc ccctatatac aatttgctta cattattcgt
     9481 cttatctatt tcttttgcta tattaataga agtttgatat cttgtatctc cacctattct
     9541 ctgtactgat atttttgaat ttacagcttt gatagcttca acaactgaat ttggcattga
     9601 gttgtttcca cctatcacaa ttacttttga tggatttagc cttttcaatt cttctacagt
     9661 tttttgagga agcttttcat ttgatgataa aagaatcggt gagtttttta cactagcaag
     9721 tggtgtagct gtaagcccat ccaccatgct tttatcttct ccatttacta ttacaactgt
     9781 atctgatttt gtccaaccac tttgagatat tttggtagct gtctcaaatc tatcagcacc
     9841 ttttaatttt ttgactgtga cattagatgt agaattactt gaacttcctc ctccagaact
     9901 tccaccacta gacgagccgc ctgaattact tccaccacca ttatttccac cattgccatt
     9961 atctggtggt aattcatccg gttttgataa ttcattttca actatgtatt ctaaactttt
    10021 tggaagtgta acttcaacat atttatttat tgtagctttt cttcccttag ttattttttg
    10081 tgctaatcca agttttaata ttacatttga accactttct tgtgtttctt tactaacaat
    10141 agaatagttt atcctaatat tgtttaataa atattcaagt gcttcacttt tctctacctt
    10201 atacttctca gggtcatttt tactagaacc agttccacca attggtttta cttcactctc
    10261 attaataagg gtaaatatta atccttcatc agagtttata ttttcataat ccactgtttt
    10321 gttgttatat gttacaattt ttaaagctgg tttatcgctt gttccttttc ctaaaacttt
    10381 atagtcagca tttgagttac tgccagattt ttttacagat attcttccta tattcaatct
    10441 atttccgtct agtaaatctt catttgatgt aacatatata tctaatgtag ttgtattact
    10501 tgatttgttg tattctactc tacaagtagc atttgcattt ccacttttta tctgagattt
    10561 tatattatca gaccaagtaa tcttttcttt ctgctcatta tctactactt caacctcacc
    10621 ttgtactttt atactggctt gaaatgaata tactatgttt tctactgagt ccacagatat
    10681 atctatagta tttactttct catacgtttt tgttccttgt aaaactggag ctgcatatgc
    10741 acatgtaaca ctaccagtta cagctactgc catagttgat actgctatag ttcttataaa
    10801 agattttctt ttactcatta acacccgtcc ttatctatca ttaactaagt ggataaattt
    10861 acttatccac ctagctttta tatttaattt ataaattacc ctaaacttat agttacattt
    10921 atatactttg cttttaatat ctactatttt aaaactattc tagtacctaa tcatattaga
    10981 tatacaatgt tagtattagt atttttacgt ttttagagtt ccacctacta tttatctcta
    11041 acaatattac cctttacttt attgcttaca gttataacct tcttattact gacatcaata
    11101 gcatttcttg tagcttttct agctttacta ttactatcag taagacttat tgtctttaac
    11161 tcatcataat tcttagctcc tacaggtaca tacattgtat cacttacaaa tctttgacct
    11221 actggcgtat ctccttgaag gtcattgtat ctatatagtt cagccttaac cttatcactt
    11281 aaagaatcaa agttttcttt tggcatatag taaacccatg tacctttact gatttcacct
    11341 aattcacctt tttctggaac ttctgcaaat aaaagtgctt ctccagatat agtcttattg
    11401 tttttatcta taagtagtgg taaattaaat gctggatttc cactgtattc acctgtaata
    11461 attattgaac ctgctggaat cttacctgtt tcatcttcac ttgtgctagg gtcatcactt
    11521 tgatatacat aatcttctgc taactttcca attccattag tacgagtttg gtctgcatta
    11581 ttcattgtgc caatttctat attatcacct ggaggtccta ttatatctaa ttctgcaact
    11641 tctatattag acttattgtt tgagattagt tttacatatc tagcttggta tgtccaaagt
    11701 ttatcatctt tattaaatgt aactctagct tcattatctt ggtcttttcc tccaccaact
    11761 aaatcttgtc catattcaaa agttccttga cttacatctt cccagttctt tttatcatta
    11821 cttacttgta tcttgtaatt agttaaagga ttgtatgttg tgctagtatt ctttctaaag
    11881 ttaaataatc cttttaatga gaacttccta gtttctgcct ctggttttgt atattttagt
    11941 cctactatag gtaatgtttc tttcatgtct actattatat atggattttc ttctacattt
    12001 atatcagggt ctttttgagg gtctttgttc cactctgctt tacttattat ctttccttta
    12061 tatgtagtac ttacatcatt atcttttata ttatcaattt caggattttg taatggtcca
    12121 tgtgagtcat tttcagttct aacatcaccg tcagaaacag tatttgttat gaatgaccat
    12181 gctgatttat ttattttccc ttcatctgat aattttacag tgccaacttc aaattcttct
    12241 gttggattta ggtgataatc atatgcaact accttatatg taaatgctct gttattaaga
    12301 ggagatatac tatcagtaaa tttggcctta ctatttcctt ttgcatccac aaatccaact
    12361 gttctgtatt ttacctgtgt tccattttca ccatctaaat aatttccatc acttcttatt
    12421 atttcgtatc ctagtatctt atctttatcc ttatctacat ctaggtctat agatatttta
    12481 ttagatttaa ctagagaacc actttgtatt ccatcagaaa aactagcact tactttagta
    12541 tcttttgcca tactagctat attttcattt aaaatctttc ttctagcttc gtcatttaca
    12601 tattgtatct ttcttgattc tttttcatag ttcttactat ccattatact gtttaattct
    12661 ggtgtaattt ctactcccca tgccttaaag taatctctta aatctttttc aacaacactt
    12721 gatgccattg ctactagcat ttggtctttt ggtaaactat taattgggtc actacttgaa
    12781 agtgctctgt attttcttga aatctttgca tagaatgtat cattacttaa atcagcatct
    12841 gagttattct ttaacattga acccgtaaaa tcatcatcat aagctagatg taattgccat
    12901 aacattccaa gcctttcaaa tacattgctt ggtataccta agctatttga agttacatgc
    12961 tcatatatct tatccattgt tgaaccttca agtcttgaat gtgtcttatc atcaaaggtc
    13021 tgagcaagta aagcaagtac attgttacta gtttcgctat atgtcatctt tccaatatct
    13081 gtaacatgtc ctatttcatg acctattccc catccaaata agtttcctcc tgtagctttt
    13141 ccactatcat ctatttggaa aggtactcca ttcataagcc ctggaactga accatattct
    13201 actcctacat gtaatccaga agcatacata aatgctccaa taaacattct ttggtatttt
    13261 atgttcatac gagattttgg agccatatca tttttagtta agttaaattc attatcatct
    13321 acagttccat taccatctaa atcttgtgtt ttaaatactc ctctttctga gtaaactaaa
    13381 tccattattt gttcccatgc aagcatacta ttatataatc tatctacttg tgtatttaaa
    13441 tcagcatttc cttttgatat tccttgatat acagctgttg caggaacatt taatgttact
    13501 ttatcacttt caatatctgt agtgtttaac acaccagttt tttctgaata ttcatatgaa
    13561 ttaccaatat ttgttcctgg ataatatggt tctcctttta cagcatcttt atatttagct
    13621 aattcttcta tatatgcttt tatctttttc ttagattcag aaactcccgc aggactctta
    13681 tcatttattg ttccatttag attaagatgt ggtatcttag tagctcctgc tacacgtact
    13741 tttatatctc ctccacttgg agtatcttca gggtatctta tgtataaact tcctccacat
    13801 tctatatcta aactatgaag tttgcttaat tgaattacat tttttccagt tttaagattt
    13861 atttctccac tcttatatgc accagattct ccatagtact gtgtatatat caacttagga
    13921 gtttttccac ttttacttcc aacatatact gttatttcat ctcctgctct agcagctact
    13981 cctaatgcct gccagtcatt ttgcatgcct aagttattgg aatttacagt atactgtccg
    14041 tttttatctt tatattttcc tgatttattt atttcttgat ttacagtagt tacatcagta
    14101 agtgtggctc tatcattata taagtcttga gctaatttta attctctatc aataactgtc
    14161 ttatatggat gatattctcc actagcttta tctggagtat ttaatctatc ttttaattca
    14221 gttatttttt cttgagttac atcttctttt aattcaactt gtaggtcatc cataaataga
    14281 tttcttacat catcttctat actgtcatac tcataaaact taagttctga tatattagac
    14341 ttatatgaat aagatgaaca aacacttact tgtatcttag tagcagttat tggttctttt
    14401 aatctaacca ttacatactt attattattt acagggtctg tctttgtttc aaaaggaaca
    14461 tcccccagat tgtgtaattg accattttca tcccaatatc gtatctcaaa gtcatttatt
    14521 gtcttatcat aaacatcaaa acgaggtgta gccataaaag tatccatagt ataagattta
    14581 tcaaaagtta ttattggtcc tctattcgtt ccctcagctg cattatatgt tgggaatgtc
    14641 caatgtgttg tgaagtctcc atctactaca ttgaatttat caaatggatt tttatcacta
    14701 tattcacttt tgtcatatcc tgctggataa acaacatcta caatgtgatt tgttggtatt
    14761 ccaaatccac cattctcctt aggagtatta attaatctat agttaggtgt tgttggtgca
    14821 tctatgcttt cagttgtacc tatatatgtt ttagacattc cactagttcc caagtggtta
    14881 gtagctgtca ttctaacttc atatgttgtg ttatccttaa gaccttctat ttttaaactt
    14941 gtcccacgaa taagcttatc attgtatttt atccaagacc cattagagcc tttttcttta
    15001 tagtacaagt caaagtcttt agcttgttta tgatttttcc agcttacatc aagagattta
    15061 aatttacctt taacatttat accttctggt ggttctggtt ttgtgtctgg aacaacttga
    15121 aattctaata tgctatctgg gctaaattgt ataggtttat agtttgagtc tacattttct
    15181 ggtactccat ctaagttttc tggcgttgca tctttataac cactagacca atctccattt
    15241 aaagattgta tgctaaccct atatagtcca tatgcttcta tatctctaac ttctattgta
    15301 tttttactag tttgtaattt tttagtagat aatacttttc ccttactatc aattttatct
    15361 agtataagtt cgtatgctga tacatttact tcattattcc aataaaaaga tactttttca
    15421 tttccaacag cagtgctagt cgatatttta tttattttag gtatgttcat ctctggcttt
    15481 ggtatttctt tatatacatt gtttaaaaat tcaacttttg atatttctgc aagatttcta
    15541 tcgtttgctg ttgtagctgt tatttttata gttatctttt ttactgctac ttgtttacca
    15601 agatttatta ctatatcgct attttgaaca tctgtatatg tttctaatgc tgccctacta
    15661 tatgtagatg ttgtagagtt atcatcttta ctgatattat ccccttcttt tggagtatct
    15721 tcatctgaat taggtttatc cattgatgaa ttttctttat cattattttt atcttgattg
    15781 gtattagagt ttacttcttg tttatcgcta ttttcctgtt gtttatcatt attttcatct
    15841 ttattatttg aattattatc agtctgattt ttattttcat ctttattatt tacttcttct
    15901 tgatttactt cttcttgttc gttatttact tcctcttgtt gctccttatc ttttaagttt
    15961 ttcatatatt ctttattttc ataaggaatc tgttgaacat taccatcttc gtcagtaacc
    16021 tcaactattc cttttgctgg agcagtacct tctggagctt ttatattgat gacttccata
    16081 ttaagtgctt ctgataaagc tatatccatt actgctggtt tttcctcaga tatttcatca
    16141 ttttttgaag atattgtaac tccattattt tctccactaa ataaattttc tacattacca
    16201 ttaatatcta catcactatt tccttttaat tcaaccttac ttgtatctac tataggattg
    16261 gtagatacta tactttctag gctttttgca tcttcacttt gtcctaaatt tttctgtaca
    16321 taatctaagt caacaatatc tacctttcca tctctattta aatcaaattc tcttttattt
    16381 gaatctatat tctctaaaac cttattataa tcactcttat ctactttacc gtctccattt
    16441 acatcaccca ttaagaataa ttcataattt aaaggtttat ttttattatc tacacttgct
    16501 ctattgctta ataagactct ttgtgaataa cttgatatat caatgtcttt aatttctttg
    16561 ttcttaaatc cagctccaga gacttctata ttgtattttc caacaggtaa attattaaat
    16621 acaatattat aatatgatac attctcatct ttatttttta tttcacttct acttgcagat
    16681 aattttttta tagtatattc tatattatct gtcccaatat ttactgttcc tgaatctttt
    16741 tctccaagct ttattattcc actattgtta ctatcatctt ttaatcgtaa ctgcatatta
    16801 gtcatacttt ctgactcgct attttttatt ggcagtgaaa aatttaaatc tacttcaaga
    16861 ttccccctta acttttctgt aactgatttt tcaactgccc ttagcttccc ctctaatata
    16921 ggttctgcaa aaacatttag tggtgcacta cacatagata ctgctagtac acaagatatt
    16981 gcctttttca tgaaacaaca atcctccctt aataaatctc taatttttca tgaatttaaa
    17041 tactataagt ttcagacaat aaaaaaagac actcaaagag aatcttaatt ataaaataaa
    17101 aagactttac tctttttagc aactggctga tatggcttta aaagctttaa tccctatagt
    17161 tttgcgtcat tagatttccc taattttgcc atttaatttt cactactttc ttacccattt
    17221 tatactatat ttaaactttt taatacaatt tttttacatt aaattataaa aaaataacat
    17281 ttatattaat aaattaatat aaatgttatt ttccattata atttaattat ttatatagct
    17341 tccttacaag cataattttg tgtatatttt cttattcttt atatcttcca taaaattcat
    17401 gtgtagcata tagattctta tctcctgtta tagctatacc aaatcccatt atttcatacc
    17461 ctttatgcaa catattagtt ccttcttctg gatttgtctt ccactcatta aaaataaagt
    17521 ttcctatatc atctgcatct ctatttgtat atacaggttt atctgaaatt ttatatttaa
    17581 ttatattttc accttgaact gctatatatc caggtttaat ctcactccaa tctaagtatt
    17641 tcaaaaaagt agaatatgaa ttttttccat ttatcgtatg agataaagtc ttcttatttg
    17701 ccattaaatt agaccaatca tttgctagac cctctaatct gcttgctaca gataacgctc
    17761 ttactccatt atcttttcta tactgctttg taagagcata tactttaccc tttattctct
    17821 ctttaaatgc tctatcattt ggattgtaat ataacccttg ttgatttggt gaagcttgat
    17881 ttatacattg cttgataaca ttctgattta tgcctcctag tacagtcaat ttgtctgcac
    17941 cacttaaaat actcttgtta ctgccatttt caactaaaac tacaggagca ttttttgcta
    18001 ttgtagaggc gaccaaagcg tctgttaatt gcaagccttt acttagataa aattctttac
    18061 tatttttata aaactcttgt ataactatct tattagtttc aaatctatct gtgccaccca
    18121 atctaataga atttgtgctc ttaactaatt cgtcgctgat tatttcacta gaaccaagcg
    18181 cataactctt taagcctgtc gtgtcaaatc ccacactatt tccatctata agtattatag
    18241 gtactccatc tctagcagct actggtgatg cacttatcgc atctgcttct cctgtaaatc
    18301 cattggctat aattacttta tctattggct taatttcttt gattttttca gctattttta
    18361 tacttgtatc aaatctattt tcaccaaata ttctttgtac aaccttcttt ttggataata
    18421 actgattctc aacattttta cttattgttg attctgagcc tattatatac acagtctttg
    18481 ctttgtttaa tctttccata gttgtgtttg gaatctgatt ttgctttgtc attaaaatag
    18541 gcgcatttaa ggctccagct aaaccacttg cacttagacc atctgctaag ctattatctg
    18601 agttgattag tattgctgta ttgtagtttt gcttatctgc tatttttgct gctgtctcat
    18661 atctgtcatt cccttttatt tgctcaactg atgtcaacgc atttaccata ggagtattta
    18721 ccattactaa aaatagtgta agtcctatag ctaatatctt tttactattc ttcatattat
    18781 tttctacccc caatgtactt ttcatatttt ttattagtac tcatacttat ttttaatcaa
    18841 aagttacaat tttgttacca tattttatat atgataccat agttatccat acaactcaaa
    18901 caatatacct aaagttttta ttctctataa attcaaaaca ttataattta ttttatttaa
    18961 ttaagttata agttcaaaat aatttatgtt agtaatatgt ttaatcatta taagaagtat
    19021 ctttttataa taatttttgc aatatgaatt tctccacatt ataatcaaaa aataaaataa
    19081 ggtatctcaa tctaattcta aatgagacac cttattttta ttattttatc ttgttatttg
    19141 tatattttat aatatctaca ctatgtctta catattaacg cattgtataa tcatatatta
    19201 tctaaataaa cttttaatat taattcctat atcttattag ttcttagtaa ataagtttat
    19261 tttttctgct attttttcaa tagcgttttt agctattcct tcacctattt gtactaactt
    19321 agtagcattc ttaagtctta actcaactac ttcttcttgt gatttagaaa gttcttctgt
    19381 agttaatact attggagcct ttgaactagc tgcaagtggt gctgctgtaa gtgcatcaac
    19441 taattgtcca tttccaccta cataaccatc ttttgctaca tatgctttag ttgcacctgc
    19501 atagtattta ttgattactt tagcattagt atcttttcta ttttctccag ctaatctttc
    19561 aactttatta tttaaatcta tatcaactaa tttgtcttca acttcttttg atacagaatt
    19621 ttctccaccc actatttcta attcttttcc ttttaataat ttttcagcat ttgcatctaa
    19681 ttcgttacct gtaactatta ttggagcacc aaattgagca gctctagctg atatactcat
    19741 agcatcagct tctccatttc cacctactac aaaagcagtc ttaaatgtat cttttaattg
    19801 ttttgctatt tctaaagaag tttcaaatct atcttctcca gaaactctct caactttact
    19861 tacacctaat ttagaaagtt tttcttcagt ttcttttgat actttagatt ctccacctac
    19921 tatatatatt ttcttagaag atagattact tccaagtact cttaatattt cagcttctat
    19981 ttctgatggt agtgaatctt ttttagataa taatattggt gcattttttt gagaggcaag
    20041 tggtgctgat gctaatccat caactatagc atcttctcca actaaaatta tagcttctgc
    20101 ttctccatct ttgaatctct ctttactaac ttctactgct gttgtaaatc ttgaatcacc
    20161 agataaaacc tctactttta attcttttcc atcttttaca tcagttaata ctttgtgtaa
    20221 gtctctaact aacttatggt ttttacttga aacacttatt gtttctgact tttcatttgc
    20281 cttgcttaaa actatatcaa agcttgattt ttctggttta tctttatcat cactatcttc
    20341 ttgttcttga gtaaggattg cttcattttc aaatgttaaa gcatatccat ttttaacttt
    20401 attaactatt tctcttccta atgtagttaa tctaattcca tcaaataatt cttctgctga
    20461 tatgtcttgt aaatctgcgt ttttaactgt tatcgtatgt aattcttttg aatctatgtc
    20521 actataactc ttttcataac cttcaaaagt accttcttct gatgttatca cttttgagaa
    20581 atctaaatgt tggtctccta catttaatgt aataacattt gctgaatctt ttgctctttt
    20641 tacttcaact gtatttgtag attttatagt tgctgttaac ttgttatcag aatcttctgc
    20701 taaagttgca ttgtaagcat taactgcatc tactatttct tgagcagttt tgtagttttc
    20761 aattttatag tcaactactt ttccacctaa cacttgatgt cctttatctt gaatagtaac
    20821 ttttaaactt tgcccttctg ttaaagagtt tattttattt tgtaattgag ttgcattagt
    20881 taagtttaca ttgtctactt ttatatcgta aactctatca tttacatttg ctcctgcttt
    20941 tgtatcttca aacttaactt ctaatgcttt tctaacctct tcgattagtt tagcagaatc
    21001 tttcgcactt actgtgtaat ttttatcaac attttctgtc acagtattgt ctgcgaatat
    21061 tggtgctgct gcactcataa ctgtagcacc tgccattact attgccatac ttctcttttt
    21121 gttgcttaac atatttatgt cctccgaata ttatttttta ttttttatat tcgtatttcc
    21181 cacacataaa taatattata tataatatta tttttcaata tattttatgt gtttacataa
    21241 tttactaaca caaacactaa taatatcttc ttgaacccct tccccctact tagctctcta
    21301 taattatgct caaagataga aatccctatt ttatagtgat tttctactaa acaatcatat
    21361 actaacaaaa gtattcaaac tccattttta tatactacta tgcactaatt tttgctttgc
    21421 tcttattact taaatatata tcactaaatt aatatctacc tccatatatt aaaatatata
    21481 acatattata aaatcatcat aaattagagt ttaaaattaa aaattattct aatacatgta
    21541 attgataaaa aataaaatat aagagtagct actttaaaat aaatgctctt atatgattag
    21601 atatttttgc ataattaaac gataagaaat caaatttaat tttacataag tagaatatat
    21661 atttttatat aataattgaa aataaaagag gatacctcat aatgataaaa gaatcacttt
    21721 gagataccct cttacaaaca actaaatcaa taattttcta tctttcacac tcttgatttg
    21781 caactgtaca agaagtttta caagctgatt gacaagaagt ttgacactct ccacatccac
    21841 cttttgcagc actttgcttt aaagtagctt gagataaagt ttttacatgt ttattttcca
    21901 ttttcaacgc ctccatttct aattgtttcc tattgattat atcataaaac tactttaata
    21961 taaactctat ttaaagttga cacccaatac gcctccaatg cctcctgctg ctgatattag
    22021 caataactta taaatcatcc ctacttcaaa tacaaacttt tcttgtgcta aaaacacagt
    22081 caaaaaaata catactatgt acaaaagtcc tactaaaagt ccatacataa gtcctttttc
    22141 tttcatgtgt tttgaagtgt aaaaccctcc tatagctgca gaaatagtag ttataaatga
    22201 tgagaccata gttatattat ctccaccaat atctgtaaat gtcaaaaata agttatacac
    22261 taataaaaca gccaaagtta ttatatatgc atatcctaga cctttaaaaa tataattaga
    22321 ttttcccata aaaaaacctc ctctcaaatt aatatatatt cgagaaaagg cttttaaatt
    22381 actaatttag tcttcttcta tttcttcttt tttagattta acagttccta tagcccattt
    22441 ttcaaatgga actttagttc tatttggtcc taattctaat attacaacat catcttcaac
    22501 tttagctaca ttagctacta ttccacctat tgtaatgacc ttgtctccta cacttaggct
    22561 acttctcatt tcttttaatt gtttgtcttt tttcttttga ggtctaatca ttaggaaata
    22621 aaagattgcg attactacta cccatagacc tatactcata attatttgtt gctgtggcat
    22681 taatttttca ctcctttgta aattatactc ttttgtaaat cataataaat gtttatataa
    22741 caatttactt tctctatctt tgagtactat tgaatattat atattaaaat gattataatt
    22801 aatagtgcaa aacctatatt tcatatccat attttgcaaa aaattcattt ctaaaatcaa
    22861 gtaatctatc ttccattatc gcttgtctta tttgtttcat taaatttaat aaaaagtgaa
    22921 gattatgagt agttattaat cttgcaccca gtatttcatt tgcttttata aggtgtctta
    22981 tataagctct tgaatagttt ttacaagcat aacaatcaca ttcagggtca agtggagtaa
    23041 agtcttcagc ataagttgca tttctaacta ctactttacc ttggctagtc attgcagttc
    23101 catttctagc gatacgagta ggtaggacac aatcaaacat gtctactcct cttataacac
    23161 cttccactaa gtcatctgga ctaccaactc ccattaggta tcttggttta tcctgtggca
    23221 taagtggagt tgtatgctct agcacgtcat acataagtgg ttttggttct ccaacactaa
    23281 gcccaccaat agcatatcct ggtaaatcaa tactagttat ttcttttgct gattgttctc
    23341 ttaagtcttt atacatccca ccttgtatta taccaaataa agcttgtttg tcggtattat
    23401 tatgggcatc tttacatctt tttaaccatc ttgttgttct ttctaaagaa ttttttacat
    23461 attctctatc tgatgggtat ggtgcacact catcaaacgc catcattatg tctgaaccca
    23521 atgcattttg tatttccata gctttttctg gagttagaaa atgttttgaa ccatctaagt
    23581 gtgacctaaa ttctacacct tcttctttta tctttcttaa aggccctaag ctaaatactt
    23641 ggaaccctcc actatctgta agtattggtt tatcccagtt cataaactta tgaagaccac
    23701 cagctctttt tataagttca tgacctggtc tcatatataa atggtatgtg ttactaagta
    23761 ttatttgtga accaatctct tttaattctt ctggtgtcat agattttaca gttgcttgag
    23821 ttccaactgg catgaatatt ggtgtttcaa taatcccatg tggagtgtgt aatcttccta
    23881 accttgcacc actctgctta caagtcttta tcaattcata tcttactgcg tacataaagt
    23941 tctccttaac catatttatt tctcattatt tcttaagata tatttgtaat aagctattca
    24001 tataatttat tgtctaaata gatttatctt cgtcttcttt gttctgttgc tccttaattg
    24061 cttcttcatc atgaatactc attatattaa gagcactctt gtcttttgtt actaaaaact
    24121 tttttatagc aaatagtcct gctatagcta taactcctat taaaacatct atcatacctt
    24181 catttttagg cagtagcaac atttttctag ctattgcgta aagtaatgcc tcaataaatg
    24241 ctcctggtat atgaagagat agcattacta caagttcaac acctattact aaaagtaata
    24301 tttgtgctaa aaaatcatta agctgactgt attgtactgg attttgaaaa tcaactatat
    24361 atgctcccca cataagtctt agaacatcta tagttcctaa aaatactgct attagaacta
    24421 ctactgctaa aacacgttca aatataaaag caatctttaa tatagggtta ttcttttttt
    24481 gtttttgtaa actatgcatt cattacctcc ctactttata atcatagcat ctccaaaact
    24541 gaagaatcta tatctttctt ttactgcctc attatatgca tttaaaacat tatcctttcc
    24601 acaaatagca cttactaaca ttattagtgt agattctggt aaatgaaaat tagtaataag
    24661 tttatctaaa actttaaatt tataacctgg atatataaat atttctgtcc atccactagt
    24721 ttctttcatc tttccatcct cattacaagc tgattctata gttctacaac tagtagtacc
    24781 aacacatatt acattgtttc cattttcctt cgctctattt attttgtcag ctgcttcttg
    24841 gctaaccata taatattctg aatgcatctt gtgatttaaa acatcttcta cttttactgg
    24901 tctaaaagtg cctaaaccaa cgtgtagagt tacaaaagct atatctacac ctttttcttt
    24961 tattttattt aacaattctt ctgtaaaatg taatcctgct gtaggtgctg ctgctgaacc
    25021 attatgcttt gaataaacag tctggtatct ttctttttca tctagtctag cagtgatata
    25081 tggaggtagt ggcatatttc caagttcatc caatatttct tcaaaaattc catcataaag
    25141 gaattttata attcttgaac cttcatctga taaatctaca acttctccaa ttagctttcc
    25201 atttccaaaa gaaaatttag tccctacttt tgctcttttt cctggtttta ctaaagcctg
    25261 ccaagtatct tcctctgttc tttttaataa taaaaactct attttacctc cagtttcaag
    25321 cttctctcct attaatctag ctggtataac tctagtatta tttaatacta aacaatctcc
    25381 tggatttaaa tattctatta tatctctaaa tactttatgt tcaatattac ctgtctcttt
    25441 atctaatacc atcaatcttg agctagctct atcttctata ggaacttgag ctataagttc
    25501 ctgtggtaag tcaaatttaa aatcacttgt tttcaatgtt aaacttcctc tcttaaaatt
    25561 tctccagcta caataatatc aaaaaaacaa cttattttcc acaatattac cataatctat
    25621 caagaaattg ctatttttaa cagacaccat atccacaata tatagatatt tttccacgat
    25681 atacatgtct tatccacaat atatagatac aatacacaca atttttatcg tgtttatcca
    25741 cagtatctac agatataatt accattttgt ggatatattt gaatttttag ccattttaga
    25801 aatattttaa tttaatgact cttttatttt ataaatactc gtataatcta tttttatttt
    25861 tcattaggat atggaatttt taagtgttca tatgctaatg gcatggctac tcttcctcta
    25921 ggacctctat ttataaatcc taattgaagc aaatatggct catatacatc ttctattgta
    25981 tttctatctt ctccaataga agctgccaat gtatctaaac ccactggtcc tcctctaaat
    26041 ttttctatta tagtcataag aagcttctca tctacaaaat cgagacccaa actatcgact
    26101 cctaaaagtt ccagagcatc ttttgccacc ttatcagtta tttttccatc agctctgact
    26161 tgtgcaaaat ctctaactct ttttagtaat ctatttgcta tccttggagt ccctcttgaa
    26221 cgttttgcaa gttctaatgc tccattagac tgtatctctg catctaagat gcttgaagac
    26281 ctaagtacta tttttgaaag ttcatcaact gtataataat ccaatttaca aataacacca
    26341 aatctatctc taagtggatt tgtaagcata ccagctcttg tagtagctcc aataagtgta
    26401 aatttaggaa ggtcaagtct tatgcttcta gcagatggcc ctttccctat tattatatct
    26461 aggcaaaaat cctccattgc tggatataaa acttcttcaa cacttctatt gattctatga
    26521 atctcatcta taaataaaac atcattttca tttaaattcg taagtatggc tgccaaatct
    26581 ccagctcttt ctattgcggg tcctgacgta attcttaaat ttacccccat ctcatttgct
    26641 attatacttg ctagtgtagt ttttccaaga cctggtggtc catataaaag cacatggtct
    26701 aactgctcat ttctcgattt agctgcttct ataaatatac taagttgttc tttggatttt
    26761 tcttgaccca aataatcttc taaagttttt ggtcttaaac tgttttctat atctatatct
    26821 tccatcttca ttgtagaagt tattatccta ttttcatctt caaaaccttg catttatacc
    26881 cctcctctcg tacttttgaa aatctacttt atatattttc aaatttacat aaactcctta
    26941 aatattgttt taaaatccaa atattataaa atccaaatac tatttcaagg atttactcat
    27001 tatatatgta agagctttct ttataatagc ttctgtattc ataccatctt ttttacattt
    27061 ttgaactgcc tcttttgatt ccgacaaagt atagcctaaa gctacaagtg catctacaga
    27121 ctcatcttgt gatatcaagg taggtttttg tgataataac gttggttcaa actctatgtt
    27181 atttttgtct accttatcct taagctctaa gacgattctt tctgctgtct ttttaccaac
    27241 accaggagct ttggataatt ttccaatgtc ttcacttaaa atatatgctc ctaactgtgc
    27301 tgatgatgca aatgatagta ttgaaagcgc caccttaggt cctattttag atacagatgt
    27361 tagtaattca aacatcttaa gttcttccct acttacaaaa ccacaaagac tcatatcatc
    27421 ttctcttaca attaatttag tatatatttt tgtggcttct cccacttgta aattcattat
    27481 tgtattggat gatacattta ttttatatcc tatcccatta ttttcaacaa ctatactgtc
    27541 tatataaatt tcttctactg taccttttat ataactatac atacttacca cctatattct
    27601 ttagagtttt ttcaagtttg tttgaatgag catggcaaat agccacagct aaagcatctg
    27661 caacatcatc tggttttgga acttttttta gacttaaaaa tgaagttacc atctgttgaa
    27721 cttgtgcctt atctgctctc ccatatccaa ctacaccttg ttttacttga agtggagtgt
    27781 attcatatac aggcttccca ttatgagcac atgcaagcat agtaacacct ctagcttgtg
    27841 caactgttat agctgttttt acattcttgt taaagaataa ttcttccatt ccaacttcat
    27901 ctatattgta attctttact attgtatcaa ttcctttata cacaagttcc aatcttctcg
    27961 atatattcat atgggcaggt gttgtaactg ctccataatc gattacttta aacttgctat
    28021 ttttgtattc aattatacca tatccaacta tggctatacc tgggtcaatc cccaatatta
    28081 tcatttttta aaattccccc taaaaataaa agaaatacca aaatgtattt ctcataaaaa
    28141 cttatgttct aaatcattat atcataaata atattaacaa atcataaaat gctctatgta
    28201 aaatataaaa tcaaataaaa aagttatatc aaagcaagat tttacctcaa tataactttt
    28261 atatttatta caaataaatt attgtatttt tataggaatt aaatgtgact acaattttaa
    28321 tactaatcta tttaatatta aattatttat tattaactta cttagtattt aacttgaaat
    28381 aatattaatt tactattaac ttgcaaagtc ttctaatctt gaataggcct cttcttcagt
    28441 atccataact acaccttttt tgatttcatc ttgaactgtt ttaggaagtg aatctactct
    28501 tatttttgtt ttttctacta atgttttact tccactatca tcatatttat atagtgctat
    28561 atttccatta tcattttcaa tagtatattt accttttata gtagcgttca ttgcttgtac
    28621 atctttatta tcactatatt tagattctac caatatgatt tcatttttat ccatactctt
    28681 tattttttta gttggatatt cttttttaaa atatgatact atttctgatt ttgtcttatc
    28741 taataatgac tctggaacta tcccagccat tgtaagtgtt ctcttatagt tagtttcatt
    28801 ttcgccttca ctttgaagcc aaacttcaca atttttattt aatccaaaag cttcttttga
    28861 cttttcgtcc atattcgcac tagcacttac aactttacta gtttgaactc cattattttt
    28921 taatcttgtc cctatataaa ctccgctact taatacaaat actccaacca gtattaaggc
    28981 agatattttc caaggaaata attctttttt cttcttttca aacattgatt acgcctcctt
    29041 tttctattat tttctccaag ctgtaagttt ttatacaatt taggtaaatt ttagaagaaa
    29101 atatatttat atttcaaatt actctaagaa tagatatttt ctaaaaggta gttttctctt
    29161 aatggttata ttcttgtaat tatatggaca taatttataa taaataatcg tttaggaggt
    29221 aataactttg gaaagaataa actgtaatgt aattaactgt tcacataaca acagccatat
    29281 atgttatgca gataaaattt ttataaatgg tgaaaaagct cacaatgaca gacatacttg
    29341 ttgtagttca tttttagatg aatctattta tagtaattta tctagcaacg ttaataatag
    29401 aaattgtgag gaattagttt gtactgtaaa aaattgtaaa tacaacaaag atggtagctt
    29461 atgtacactt gataatatat tggttgctcc tgaaactgat agagtaaacc tttatattga
    29521 aacatgttgt tctagtttcg aatgtaaata gtcatagtat aaatataagg gtgtcaaatg
    29581 taattaaact aacatagtgt agagtatcta caactattag tttgattata cttgacaccc
    29641 ttatttattt taaataaaat ttcattatta taaccatgtt tataatgttc taactgtaat
    29701 taaatttgat aaatttctat aaattggtat aatcaatatg taaattgaat ttagcaaatt
    29761 aatcataaaa ataaaatatc gagtatatag tgttatacta actagaaatg aggtgcttat
    29821 gagtacattt tatattcaat ttctttattt tgtaatttat agctttatag gatggtgttg
    29881 tgaaactaca tattgttcag ttttacaaaa acaatttgta aatagaggtt ttttaaatgg
    29941 tccattttgc cctatatatg gatttggagc attagcaata gttgctacct tgactccttt
    30001 tgtacacaat attcctttgt tatttttgtt tagtataatt ataactagca taatggagta
    30061 ttgtacgagc tttattttag aaaaaatatt taatatgact tggtgggatt attctaaaca
    30121 taaatttaat attcatggaa gagtttgtct ggaaaactca cttatgtttg gtgtattatc
    30181 attgattgta atgcttatag tacatcctat tgtggttgac tttattaact ctatctctaa
    30241 aaatgtgcta tttatatttg ctatatctat agaaatttat tttgttttag atttagttat
    30301 cacagtccat acaattttac aattaaatgg taagttaaaa caaatcaatc ttataattaa
    30361 ggaattaaaa gataagaaag aatactacaa acttattaca caagaaacta tagaaaacaa
    30421 acttgaatct ttagttgaaa atagactgga tactttggaa gatagatttg aaatacttaa
    30481 ggaaaaagaa aactatcgct atgctaaaaa tcgtattgat gagctaaaaa ataaacttaa
    30541 tactttgctg tctaaccaca aattacttca tagacgtata atcaaagcat tccccaacat
    30601 aagctctaat aagcatacag atattttaaa taaaatcaaa gaaaatatta aatcaattaa
    30661 aaaagataaa taataaaata aacacataag ttaaataata aaaatactaa tacataagat
    30721 taaggtttag aatatttttt gtaaaatact ctaaacccta attaatatac tattattttt
    30781 aatcttaatt tataatttta gtttatttta cttttaacac agtaaattta agcgtttaaa
    30841 cttcaataca ctaatatact accccatcaa tatctcaact gattctctaa gtgtgctaat
    30901 ctctcccaca tttccaggga aaattatata tggcatatta ggaaatttgc tttctttgcc
    30961 tgtcatccat actggtatac caggttttat ctgtcccatt actgtagctt tttttactct
    31021 tagtgcttta gtaccaacat cactagaagt aattccaccc ttagctataa taaaatttgg
    31081 tcttacatta agcataccaa ttatactggt aactgcatct gatatttcta cagaaatcat
    31141 aagctgttta tctttatcat tcgtatccaa gtcaaaacgc tctcgtctag tataaacagc
    31201 aacagtttta ccactgctta ttttttcctc tacaactttt ataactcttt ttacctcatt
    31261 ttttagacca ttttcctgaa gaactaaatg ttgattaaat tctataaatt ctattggata
    31321 tttacaattt ttaagttctt ctaactgttg ggtagtctta ttcacatgtg aaccaacaag
    31381 tataattcct ccattggtat taccttttga tactaactca tccttagtaa gcatttcctt
    31441 atcactcact ccgccaagta cttttgttat ggcagcagcg cttctaaaca taaattcctt
    31501 accactattt actgctcgta taaacgcaat tgtaaatacc tttacatcta cataatcggt
    31561 tgcatttaca ataattttat tgaatccttc agctttttta agtttttctg ttatagcttc
    31621 aatatttagc tctctaagtt ctttagaaga aatatatatc atatcactag atttgtactt
    31681 tccatgtgtc ttttcttcac accactcacc tatatcagat gatttatatc caaaagattt
    31741 atcctttgca aattcagtca ttcctgctgg tacaagtatt tctccttctt taacataatg
    31801 tacattatct atagtaaacc taccaccttc tttaaaaaat ggcattatta tttcaccctg
    31861 aaataacttc cccgataatc tctctacttc gctttttaat aaattagttt caacaggata
    31921 atgtcctctc atagtagaat cactacgact tatgattata aaatccttat taactttctt
    31981 tgatacatcc acaatattct ttgaaatttc tttatgtgct tttgtagttt gagcaacagt
    32041 aaatcccctt gaattagtaa gtataaaaaa catactattt ttctcattaa aaccttgttc
    32101 catactatcc ttatcccaat cagtataaac agaaatatca tgaactgttt gtacacctgt
    32161 tgggtcatca tctaatacaa caatttttct atctaaatgc ttaagctcat ctgcaagaag
    32221 ttcatttaat ctgtcctcat ctacttgttt aaaagtatta agtatatcta tgctaattgg
    32281 ttttacttct ctcatttaaa acgccccttt atgtaatttg tatagtctaa atgtaattta
    32341 tataatataa acaatagttt gttatttata gacctggaag tggtataatt ccatttataa
    32401 cactaagcaa taaaaccatt ataaatgcta caactccagc aatggttcct ccaattgtcc
    32461 aagagtttaa agtatcttta actgataatc cactaaatct tgaaagtacc cagaatccag
    32521 aatcatttgg aagggagaaa ccaactcctc cagcacatat agcaagccct actaaaactg
    32581 gtgacattcc agttgccaaa attgtaggtc ctaaaattga tgatgttgtt actaaggcta
    32641 cagtagcaga accttgagaa attctaagca aagcacttaa taagaatcca agcactacaa
    32701 ctgatatact aagaccagac atagtatcaa ctaagaagtt acctatacca cttgcattta
    32761 taacactgcc aaacgcacca cctgaacctg ttatcaataa tattagccct gctgaatcag
    32821 cagcttcaat aactacatct cccataggtt tatttatgta tctctttaaa aatcctattg
    32881 ccaccaatat tccaagaagt attgctatgt ttttatctcc tataaatgca agtactccac
    32941 tcattgtaga acctttttct aagaacatac taaatacatt tgatataagt ataagaagta
    33001 ttgggaacaa taaagaaaac atagaaattc caaaacctgg tactttagaa gaatcatcat
    33061 cttcaatttt tatgctatct tttccctttt ctaaattttt ttctctatca ttctcatcaa
    33121 tagcataagc tggatatttt tcaaaacgtt ttccataaat ccatccacct ataagaactg
    33181 ctggaaaagc aacaattata gaataaagta taaaactacc tacatttgca tttacatttg
    33241 ctgcaactgc taaaggtcct ggtgtaggta ttacaagtgc atgacctact atagtcccaa
    33301 ctcctaaggc acatacaaaa accattagag atttcttagt tactctcgaa acatacttaa
    33361 ttataggcat cattataaca aaagctgcat ccataaaaac tggaattgaa attaaaaaac
    33421 ctgctgtagt tactgctaaa gcactgtttt tttctccagt cttagctaac aacccctttg
    33481 caatagattc tgttgctctt gattctgaaa gaatatttcc aaatattatt ccaagtccaa
    33541 ttacaatacc tatactacct aatgtatttc caaatccagt cgatattgtg gttgatattt
    33601 catttatagg cattctaacc ataaagcctg taattataga tgttatcagt aatgccaaaa
    33661 acggatttac tttaaattta ataatcgata caagtaaaat tcctatagat ataacaaatg
    33721 ctatcaaaag tccatatcct gtaatcataa tctttacccc tttatcattc tactttatta
    33781 tttatcaatt ttatttttct tttactatga taacttaacc tcgctgtatt tatataatct
    33841 aatcctaata atatgtattt aattatattg atatatttaa atattaatta ttgtgttttt
    33901 ttatttcaac acctgccaac ttttcaaaat actgtgctat tccactatgg tcatcttcca
    33961 aatgtccatg tacttttaat gtctgcataa tttctaataa ttgacttgtc ataggtagcg
    34021 gtacatctaa attgtgtgct gtttgcatta cattcttaat atctttcaaa ttaatagata
    34081 tttttccacc tggtttaaaa tcacgattca taatcattgg tatcttcgca tccaatattg
    34141 tactaccagc taggcctcct cttattgcct tatatacttt ttctgggtct gcacctgctt
    34201 ttgctgcaag tacaaatgcc tcagaaactg ctgctatcgt caaattaact attacttggt
    34261 ttgtaagttt tgtaacactt ccactaccat tatctccaat taaaatagat gagcttccca
    34321 ttatattaaa aaatggctct actttcttat aaatttcttc cttaccacca gccataaaag
    34381 caagtgtacc atcaatcgct tttggctccc ctccacttac tggtgagtct ataaattcta
    34441 agcccatatc ttttaattta tttgcacaca atattgattc tgtaggagta actgaactca
    34501 tatcgactac tatacttcct tcttttaaag tttttgccaa tccatccatc ccaaataata
    34561 tatcctgaac aatagttcca tttggaagga ttgtaaatac aatatcacat tcttgaccca
    34621 tttctaaaac acttgcgtat tttgctccac atgatataag ttcatccaca gcagatttat
    34681 taatgtcata caccaaaaga ttacatccat cttttaataa attttttgcc attggctttc
    34741 ccattattcc taatccaata aatcccaatt tcattttata accctccata tcttcacatt
    34801 tttgaaaacg ttttttatgt tttagagtat actacatacc agtataccgg tcaatataaa
    34861 atataaaagt atttatttta acaataaata gtaattttta aagtcattta ttatatttag
    34921 tcatcctata taagttttaa tacatactat tgtttttaaa tataatatgc tacttttaca
    34981 gttttgtatt tttactaatt tcaattagtt ttaattaatc tgcctttaat gttaatcata
    35041 cttcacactt caataatttt ttatattttt taatcttatc tgcaatttaa attgcaaaag
    35101 attgacctta ttttcactat gttgtatgct taaaattata taagtttttg tatttttata
    35161 ttgtttaggg gggtattatt aagtatggct acatttaaaa gattacaaga acaagcatat
    35221 gactacttaa aagaattgat actatctgga gaaatgatag aaaatgaaat atattctgaa
    35281 acaaaacttg ctagtgaaat aggtatctct agaacaccta taagagatgc tcttcaaaga
    35341 cttagtcaag atggatttat agatataatc ccaagcaaag gatttagaat ccatcaaatt
    35401 acagcaaatg aaattgtaga gatattccaa atacgctctg caatagaagg gttttgtact
    35461 tttttaatta caagtcaata caaagaagct agggcagtag aaactataag taaattaaaa
    35521 catcttttag ataaacaaaa agatatttta ctaggtgata aaaatttaaa ctcatttgca
    35581 gaatacgata cattatttca cacaactata gtttcatatg ctcaaaatac tgaatttgac
    35641 aaaatgttta acaactatat gtacagaata aaaaaattag ctctcgattc attatcacat
    35701 gaaggaaggc ttgaaacaac attaaaagaa cacacagata tttttaataa tattgcaaat
    35761 ggatgtattg aagatgttta caaaactact ctcattcata tggaaactcc taaatacatt
    35821 aatttagaag atttttgtaa caataagtga ttttagtatt atactttcat ttattaaaaa
    35881 tatatctacc tcaaattaat ttcctaattt ataaaaaata ttctataatt atattgtaac
    35941 taatactatt ctaaggagtg atttttttga acgaaaatgt tgtaactaat gaaactattg
    36001 acaaagatta tgctattgaa gaagttcgta tggcatgcaa acattttggt gacttatatt
    36061 tttatttttc caaagtgtta tttgaagagt ttggtgaaga taaaactatc gaaatactaa
    36121 gaaaagtttt atttgaaagg tctgaagaaa gagctattgc tatgagagaa agagctcttg
    36181 aaaatggaga tgaattaatt gcagacaata ttattagcac aactgatgtt ccatttctag
    36241 gatgggttcc tgaatttaaa gagttacatt gcccttatgg agcttcctgg cttttaagat
    36301 ttgaggaaaa cccttggttt aaaaaattcg cttctcttta ctgtgatgtt acagatacta
    36361 ctgtagctga agtatttaca ggagatacgt cccataaaat aacaaaaaac attctatggg
    36421 gagataaaag ttgtgaaaga atatatttcc atgatgacaa ggtatctgaa ggtaaatata
    36481 cttatggaaa taaaaactgt aaataaataa tttaaatttt ctatattcat atactatatt
    36541 ttgtactttt tcatataaat attataacta gtcttttatt ttacttttgt ttaacctaaa
    36601 gataatttac ttttaatcta tatgaattat catataaata atcagaaagt tatctttata
    36661 aattatagtt ttagatttat aatttataaa atatttatgt tctttgtaaa attttttaga
    36721 agcttctaat gttatgctat aaaactatct tgagaagggc tgattgaatg tttaataaaa
    36781 tatatgaaaa gaaagaattt atagtttttc aagttaaaaa aggttatgta gtttataata
    36841 ctagaaagag ttttgaagaa ggtcatacac atttaaagca ttttgaagca gcaaagacag
    36901 ctattgattt agcaataaat aaaaagattc ctagaagcaa agatggatat tatctgacta
    36961 gtttaattcg cataagtgat gatggttatt acattgataa gttaagcgaa ttgttatatg
    37021 ttagagaaca aaaaggtaaa aaagaaaagt actgtaattc tggttatcag atgtaacaag
    37081 actagttgtg aattataagt accaatcata aataccttta gtcttaaaat caaagagtat
    37141 cagtaaatct ataaaaaaac aacttactga tactcttttt aattatatga ttaatatttg
    37201 aatttgatac aaaattttat acaaaagttt atgttatcta ttctgattct ttgtctgcaa
    37261 attggctatt ataaagcttc tcataaaatc cacccttttc cattaattca tcatgattcc
    37321 cctgctctat aatacttcca ttattcataa ctaaaatcaa gtcagcattg cgaattgtag
    37381 aaagtctatg tgctataaca aaactagttc taccattcat aagatttctc attgcttttt
    37441 gaagcataag ttccagccta gtatcaacag aactagtagc ttcatcaagt atcaatattg
    37501 aagggtctga tataaaagct ctagctatgg ttaaaagctg tttttcccct agagatatat
    37561 tagatgcttc ttcatttaag aacatattgt atccatctgg aagagtggtt ataaagtgat
    37621 gaacatttgc tacttttgct gcttgtataa tttcttcttt agtagctcca aatctaccat
    37681 attctatatt ttcaaaaatg gttccattaa ataaccatgt atcttgaagc accataccaa
    37741 acattgaacg caaatcttta cgtttcatat ctcttatatc tactccatca attttaatag
    37801 cacctttctt tacatcataa aacctcatca ataagtttat taatgttgtt ttacctgctc
    37861 cagtaggtcc aacaatagct accatctgac cactttttac ttctgcattt aaatcttcta
    37921 ttaaagttga attttctcca tatccaaaat taacatgctc aaatgtaaca tttcctttta
    37981 cattttctat cttaactgga ttttcaacat caggtatttc ctcttcttcg tctaaaattt
    38041 caaatacacg ttctatagct gcaaaagaag attgtattac tgtagataac tgagtcattt
    38101 gtgaaagtgg ctgatttatc tgccatatat aacgcacaaa tgcttgtaaa tttcctactg
    38161 ttatccctcc acttactgca tatatagaac cgacaactgc cacagctgca attccaaagt
    38221 aagttactaa tgaaaccata ggattcatca tacttgatat aaattgtgcc ttaaatccat
    38281 tttcacaaag ttcttgattt acttttttaa attcttttat agaatcttct tctttaccat
    38341 atagttttat ttcattaaat ccagtataca tttcttgtac ttttccattt aattttccaa
    38401 gagcattttg ttgcaatgaa aatctacttt gagatttttt tactacaaat ttagatacaa
    38461 aataactaat tggaattata aaaagagcta taagagccat atatatatta attgtaaaca
    38521 tcattgaaag tgccaaaatt atgactaaga ttgagtttac aatttgcata aaactctgtt
    38581 gcaatgcatt tgatattgta tctacatcat ttgatatacg acttaataca tctccaaaac
    38641 tattactatc aaaataacta ataggcaatc ttcttatttt attttcgact tcatttctta
    38701 aatctctcat tgtgttttga atagcattcg taagtagaaa acttgaagta tacgtaaaaa
    38761 ttgcattagc aaaatatata catgctaaaa tcaccaatat ttttgttaca taatcaaaat
    38821 ttacggaagc ccctggtact ccttttgcga tactaattac atctttagtc aattgtgtta
    38881 ttatcaatcc ttcagtttta ggtgcaagtg cattcatagt agctgctgaa attatacata
    38941 taatagctat aataaaggaa aatttatatt tctttacaaa tggaagaagt ctaccaattg
    39001 ttttcttaaa acctttagac tttttcatta ttctaattcc tcctttttta actgagatgt
    39061 tgcaatttca tagtatattt cacagttttt taataattct ttatgagttc ccattccgac
    39121 aacttgtcct tcattgagta ctataatctt atcagcatca ataatagaac taattctctg
    39181 ggcaacaatc aatacaattg catcttttgt ttccttcttc aatttagcac gaagtattgc
    39241 atccgtttta aagtcaagtg cagaaaaact atcatcaaaa atgtatattt caggtcttct
    39301 tataatagcc cttgcaatag aaagtctttg tttttgacct cctgatacat ttgctccacc
    39361 ttcactaatc aattcatcaa actttctagg tttttttgat ataaactcat atgcttgtgc
    39421 aactcttgcg gaatattcta attctgactc tcctgcctta tgtttcccaa atctaatatt
    39481 atttgctatg ctaccagtaa aaagcagtga tttttgaggt atgaatccta tcttttttct
    39541 taaagctttt aaatcatatt ctcttatatc tactccattt atcttaatgc taccctctgt
    39601 tacatcgtaa aatctaggta tcaagttaat tagagtactt ttcccactac cagtacttcc
    39661 tataaaagcg actgtttcac ctgtttttgc agtaaatgaa atatccttaa gtacagaagc
    39721 ttctccatct ggataggtaa aagttacatt atcaaattct atgataccct tattttcagt
    39781 atccttaatt ccattttcag gattttttat caatggttct tcatttaaaa gttcttttat
    39841 tctatttgca gatacctctg ctcttggata cataataaat accattgaaa aaagcataat
    39901 tgaaaacatg gcatggaaaa gatattccaa aaatgcaacc aactgtccaa cttgaagctc
    39961 tccaatattt atcttctcac ttgaaatcca aaatacagcc aaaacagcta tatttaatag
    40021 taaaaagaat gctggttgag taattgacat aagcttaaat agtttcttag aaacattagc
    40081 ataatatgta tttgtttttt caaatcgctc tgtttcataa tcatcatttc caaaagctct
    40141 aattactctt attccagtta aattttctct cgatataagg tttaatttat ctagtccttt
    40201 ttgttgtttt gaagatattg cctgagatac ttttgctatt attgctactc caataattat
    40261 aaaaggtact gatatagcca atactataga aagttccaca cttgttctaa ttgtcattat
    40321 tatacttatg atgaacataa ctggagttaa taaggcagtc cttagtagta tattaacaaa
    40381 ttgttgaatt tgaaatgcat cattcgttgt tcttgtaatc attgaagaaa ttccaaattt
    40441 attgtattca gtatgtgaaa attcttgtga tttctcaaat atgtcatttc ttatatctct
    40501 ggttacactt gttgatacct tagcagaaca ataacccaac aatatactac ctacaacccc
    40561 tataatagaa ataataacaa tcactattcc catttgtttt atgtaagcta tattctgatt
    40621 tgcaatacca ttgtcaatta ttttagctat tatagttgga atccccaact cgactaatgc
    40681 aaaactaaat atagccagta tgtttaatac tattaataac ttataattct ttaaataact
    40741 taaaattaat ttcatatttt tctcctttct taatttttga gatagtgtaa tcatgtaata
    40801 atactaaatt tataaatgct taattttata aaaaaaggag aaaagagtta acgcactctc
    40861 ttctccagca tattcttttt aatacaccct ccgacaagtc gcttatatgt taacatattt
    40921 gacaagtaat gtcaattcat atagctaaaa gtcttttaaa tgcttcatct cataacattg
    40981 taacaaattg gcatgtaatt tatttttgtt ttttactgta ttccaatttt acaagttctc
    41041 caatattact aactttctta aaattcaaat tagtcttatc tataccagac ttaaatagtg
    41101 aaatccctct ccccaatatt attggcaata tatatatata ataatcatca attaagttat
    41161 ctttcataaa taattttact atatcgcctc caccaactat ccaaatatct tttttatcct
    41221 tcttttttcc aatttgctca attaattttt taggttctaa attagtatac tttacattct
    41281 ccagttgtcc cattaattgt atttgagtat atatagcatt ccttaccctt atagtaccat
    41341 tcatttgggc ttaattcatt tgcaatctgt acatatgtgg aatttcccat aatgatagta
    41401 tcaattgtat cgtaaaaagt attataatca tcttcctgct cattcttaaa ttctgaagat
    41461 atctcgtcta aaaaacttac actgccattc tcttcagcta tataaccatc taaacttgtg
    41521 gcaatataaa gtactacttt tctcatgttt actacctcca acatttatag tgtgattatt
    41581 ctatgtaaac atcacttttt tactcattca ataataccct attaataaat cctcaaacta
    41641 atttaaataa actttgcata aaactaaaaa tatattatat aaataattta aaatcacact
    41701 aacttattga aaattctaat tattacaaat aaatattgct ctctgttaac gctagtttta
    41761 taatataatt ataagctatt aatattataa agccgatatt actaatataa taattatagt
    41821 taatttactt ttgttgaaag gtggtaaata acatgaaaat ttcagaagtc gctactccaa
    41881 attttttgct agatttagac cagttagaaa agaatataga taaaattcaa caaatttgta
    41941 caaataataa taaacaactt tggcctatgc ttaagacaca taaaagtact tacatagcga
    42001 gacttcaaaa aaatgcaggt gctagtggtt ttctagttgg aacacttgat gaaggtgaag
    42061 ctctgataaa agcaggtttt gaagatatta tgttggctta tccttacatg ggagaagtaa
    42121 atataaatag aattgtgact atggcatcaa aaataaattt aatctgtgct acagataata
    42181 tagaatgtgc taaggcttat agtgaagctt tttctaaatc aaaaatagag tgcaagctac
    42241 ttattattgt agattgtggc ttacaccgtt ttggggttga accagaaaaa gtggttgaac
    42301 ttgctactga aatttctaag ctagaaaaca tcaagattgt tggtataagt tcgcatccag
    42361 gtcaagtgta cggttgtagt tctccagaag gtgttccaga tgtttgtgtt caagaagatt
    42421 cttctatgga gaaagcatac aaagctctaa ctgataatgg atttaatata gaaattgtcg
    42481 cttctggtag tactccaaca gttgaagctg aagctgcttc taaaactata actgctgtaa
    42541 gacctggaaa ctatgtatac tatgatgcta tacagatagg tttaggttgt gctactgaag
    42601 atatgtgtgc tcttactgtt gtgtgtactg taatctcaaa aaatagcagt ggatattatt
    42661 taattgattg tggaagtaag tgccttggac ttgataaagg tgcacatgga aatagtagta
    42721 ttgttggtta tggtagggta tgtggacatc cagaacttac tattgattca ctttcagaag
    42781 aagttggaaa aattaaagta aatggtgaaa ctgatataaa aattggtgat agaataagaa
    42841 ttatacctaa tcattcttgt tccactgcaa acttgacgag taatttatta ggttttagag
    42901 gagatacaat agaaaaagtc atagaagtag atattcgtga aaattcaaaa caaatcatcc
    42961 tttaaaaata tatttatttt gatattaaca tcatgaaagt atatttttct taaaattatt
    43021 gttaataact aatgttaatg ttggataaat aaaaaacata aattctagaa atataaattt
    43081 aaaaaaagag gctgtctcaa aattggatat gtccctgtca agtagacaga cttaaaaaag
    43141 gctatcttta tgcggaatga gttcgatatt caatcggact cattcc
//
LOCUS       DAEPLF010000010        43063 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEPLF010000010 DAEPLF010000000
VERSION     DAEPLF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43063
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.010"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(148..927)
                     /gene="hisJ"
                     /locus_tag="KR314_000512"
     CDS             complement(148..927)
                     /gene="hisJ"
                     /locus_tag="KR314_000512"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBH0326917.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEDLIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(1467..1817)
                     /locus_tag="KR314_000513"
     CDS             complement(1467..1817)
                     /locus_tag="KR314_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBH0326918.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(1833..2249)
                     /locus_tag="KR314_000514"
     CDS             complement(1833..2249)
                     /locus_tag="KR314_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBH0326919.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(2268..2459)
                     /locus_tag="KR314_000515"
     CDS             complement(2268..2459)
                     /locus_tag="KR314_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBH0326920.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(2610..3425)
                     /locus_tag="KR314_000516"
     CDS             complement(2610..3425)
                     /locus_tag="KR314_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBH0326921.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(3472..4410)
                     /locus_tag="KR314_000517"
     CDS             complement(3472..4410)
                     /locus_tag="KR314_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0326922.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDASNKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYIYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(4407..5279)
                     /locus_tag="KR314_000518"
     CDS             complement(4407..5279)
                     /locus_tag="KR314_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0326923.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEK
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(5304..7232)
                     /locus_tag="KR314_000519"
     CDS             complement(5304..7232)
                     /locus_tag="KR314_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBH0326924.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNSL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(7344..7721)
                     /gene="gcvH"
                     /locus_tag="KR314_000520"
     CDS             complement(7344..7721)
                     /gene="gcvH"
                     /locus_tag="KR314_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBH0326925.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(7808..9934)
                     /gene="cdhC"
                     /locus_tag="KR314_000521"
     CDS             complement(7808..9934)
                     /gene="cdhC"
                     /locus_tag="KR314_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBH0326926.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(9991..10797)
                     /locus_tag="KR314_000522"
     CDS             complement(9991..10797)
                     /locus_tag="KR314_000522"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBH0326927.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(10847..12214)
                     /locus_tag="KR314_000523"
     CDS             complement(10847..12214)
                     /locus_tag="KR314_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBH0326928.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(12245..13189)
                     /locus_tag="KR314_000524"
     CDS             complement(12245..13189)
                     /locus_tag="KR314_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBH0326929.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(13209..13979)
                     /locus_tag="KR314_000525"
     CDS             complement(13209..13979)
                     /locus_tag="KR314_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0326930.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(14035..15420)
                     /gene="lpdA"
                     /locus_tag="KR314_000526"
     CDS             complement(14035..15420)
                     /gene="lpdA"
                     /locus_tag="KR314_000526"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBH0326931.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVIEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(15464..16345)
                     /locus_tag="KR314_000527"
     CDS             complement(15464..16345)
                     /locus_tag="KR314_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBH0326932.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMQKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(16381..17055)
                     /locus_tag="KR314_000528"
     CDS             complement(16381..17055)
                     /locus_tag="KR314_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBH0326933.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(17092..17964)
                     /locus_tag="KR314_000529"
     CDS             complement(17092..17964)
                     /locus_tag="KR314_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBH0326934.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(18056..18688)
                     /locus_tag="KR314_000530"
     CDS             complement(18056..18688)
                     /locus_tag="KR314_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBH0326935.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKIEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(18793..20469)
                     /locus_tag="KR314_000531"
     CDS             complement(18793..20469)
                     /locus_tag="KR314_000531"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBH0326936.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(20718..21497)
                     /locus_tag="KR314_000532"
     CDS             complement(20718..21497)
                     /locus_tag="KR314_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0326937.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(21807..23726)
                     /gene="cooS"
                     /locus_tag="KR314_000533"
     CDS             complement(21807..23726)
                     /gene="cooS"
                     /locus_tag="KR314_000533"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBH0326938.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(24333..25244)
                     /locus_tag="KR314_000534"
     CDS             complement(24333..25244)
                     /locus_tag="KR314_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBH0326939.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(25360..25482)
                     /locus_tag="KR314_000535"
                     /pseudo
     CDS             complement(25360..25482)
                     /locus_tag="KR314_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            complement(25565..26707)
                     /locus_tag="KR314_000536"
     CDS             complement(25565..26707)
                     /locus_tag="KR314_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH0326940.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYKVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(26725..28479)
                     /locus_tag="KR314_000537"
     CDS             complement(26725..28479)
                     /locus_tag="KR314_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBH0326941.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMKINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            28615..28821
                     /locus_tag="KR314_000538"
     CDS             28615..28821
                     /locus_tag="KR314_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326942.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(28826..29749)
                     /locus_tag="KR314_000539"
     CDS             complement(28826..29749)
                     /locus_tag="KR314_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326943.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(30003..31703)
                     /locus_tag="KR314_000540"
     CDS             complement(30003..31703)
                     /locus_tag="KR314_000540"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBH0326944.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(32114..32527)
                     /locus_tag="KR314_000541"
     CDS             complement(32114..32527)
                     /locus_tag="KR314_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBH0326945.1"
                     /translation="MILVSACLIGINCKYSGDNNENEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKIKSEKDL"
     gene            complement(32595..34973)
                     /locus_tag="KR314_000542"
     CDS             complement(32595..34973)
                     /locus_tag="KR314_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBH0326946.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSEISAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRNLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            35290..36741
                     /locus_tag="KR314_000543"
     CDS             35290..36741
                     /locus_tag="KR314_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBH0326947.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(36945..39458)
                     /locus_tag="KR314_000544"
     CDS             complement(36945..39458)
                     /locus_tag="KR314_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH0326948.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTSDEQLEVLKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTDLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKDTEKLKVGVLLNKPPFQNADKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLEDMAKKHEIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLNGK
                     RIALAKGIDYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYEAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKSDKESENGNIENERDKIRREYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRITSKIIYNDRKIPMYVIGKIANIDSEKEEKNRLLDKVQR
                     DSLTNIYNAAYIRKLTTQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLVGDKVLIDV
                     AKVLDESFAKDDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVITKRGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(39681..40502)
                     /locus_tag="KR314_000545"
     CDS             complement(39681..40502)
                     /locus_tag="KR314_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0326949.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMIVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEMR"
     gene            complement(41021..41497)
                     /locus_tag="KR314_000546"
     CDS             complement(41021..41497)
                     /locus_tag="KR314_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBH0326950.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVGNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(41531..42109)
                     /locus_tag="KR314_000547"
     CDS             complement(41531..42109)
                     /locus_tag="KR314_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBH0326951.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(42213..>43063)
                     /locus_tag="KR314_000548"
     CDS             complement(42213..>43063)
                     /locus_tag="KR314_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBH0326952.1"
                     /translation="SISMDLDTSSSLGIPKELMGSNTNNIDTLSNTSKIDTLNKNHYN
                     TAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNIDCYL
                     NAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVTEYCP
                     MGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLDNLYE
                     LEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGTVAGH
                     YYKGVE"
BASE COUNT        12967 a         8413 c         4806 g        16877 t
ORIGIN      
        1 tttacttaga ttctattgtt attttactta gaacttattg ttattttatt taagttctat
       61 tactattttc aatcatataa atttacaaaa gactgtacat aaatttattt tatacaaact
      121 tatctacagt cttttgtgtg aatatatcta aataatatcg tatttattta tttcctctgc
      181 acagtgagaa tcagaaccta aaactaatgg aatatcgtat ttttttacca actctaataa
      241 gtatccatct atataaggct ctttgcaata ctcttttcta tttccagaaa cattataatc
      301 aagttcataa cctttttctt taattaactt tattaagtct tcaagaattg tatttccttt
      361 ataatcgtat ggaaatacct gattaaactt tctaactaaa tttaaatgac ctattctttt
      421 tggtttatgt atacctaaat ctgaatttac agcttttatc aatgtttcat aatacttatt
      481 ataaacagct tcaatacttc ctaatttgtc gattaaattt tgaaactcct ctacactgta
      541 atctacacaa taataatcat catcaatttt aattatatgt actgataaaa gagagtcttc
      601 taattccttt ccatatttat ttaaattctc ctttatccct tcttcgtatc cttctataaa
      661 atccacttcc gacccaacat ttatcttgat tttaccctca tacttttctt tcaacttctt
      721 gacatcatta aaatactttg gtaaatcttc aatactcata caactatcat tttcaggagc
      781 agggtctcta aatccatttg catacggaaa atgttctgta aatgtcattt ctgttattcc
      841 aacatccatt gctctttgta catatttttc aaaatcatcc ttacttccat gaggacaata
      901 aggtgaatgt atatgaccat ctttcataaa taattcactc cttttttaaa gaaaaaatct
      961 aatataaatg cataaatttt ttaacttcaa aataatattt ttaatcatgc taatttttat
     1021 tatatctaat attagagtaa ttttaaactg ccttttaagt atcttatctt tagtactatt
     1081 ttcaacttaa cattaatatt attttttaac ttaacattaa tactatcttt aaatagtaat
     1141 actcaatata taattaatca aataactcaa attgtttgat gaagcttttg cagtatttaa
     1201 agaatatata attatttaac attaaataat ccaatcttgt aataaaattt actcattaaa
     1261 gtatattaca gaaaatatct caaaataaac tttttagttc atgatatact cttttttgca
     1321 tgaaattaga tatattttta tcatttcaac aatgaaaggg aggcttacat ccatcttgag
     1381 ataacccctt agataaaatt tttaataatt aaacatcata aaatataaaa atcgcttata
     1441 tcaaacgtat aagcgattaa ttatatttac atatgttttt tagcctctaa caacatttta
     1501 ttagcttctt ccatatactc tatagccttt tcaatactct ttgctgtttc ttcttttcca
     1561 atagctctag ccttttcaac ccactctcta aatccttctt catgagtttc attgtgattt
     1621 atccaatgta ctaaaagtat ttttaaagtt ttttcatcct tagactctga tgattcctca
     1681 tgactatgtt catggtcgtg gtcgtgagca tggtcatgtg gatgttcatg acatatatct
     1741 ccatgagtat gttcgtggca gtggtcatgg tcatgatgtc catgatgata tcttacaatc
     1801 tctttatcaa aaatcattag ttattccccc tgttatttta tattaaactc tttttttata
     1861 aggtttacag aatcttttac aagtagtgct aatggtttac cttctaatcc aactaactcc
     1921 acattttctg gcataatagg taaaagtatc ttaagtgcct cactgtctgc aatagcttca
     1981 cttatattac aagtaacttc tcccatcata gaattaggta ttattacaga aataggtgca
     2041 actattatat ttgctttttt agctgatact actatagcat tttcccctgt agctccttta
     2101 ttagcatgag ctttcatcat agaactagtt gctatagaat tagtccctag tgcataaatt
     2161 tctacatatg taggtaactc ttctctcaaa gaagaaacta tctgggctcc aatacctcca
     2221 cccattccat ctatcactgc tattatcata tgaaattccc ctattaatta attttcttgt
     2281 attattatct tatggtccat caatcttatt tctttaatta tcccatctat tatcttttgc
     2341 tctcctaata aatccgttaa ataaatcttt ccatcacaag ggtctatatt aaccacattt
     2401 tccataacct tttctaactc attattactt ttacaaatga aagctgatga ttcacacata
     2461 taaatctcct cctaatatga tttttattaa aaatcaaaaa aagattccct taggtatttc
     2521 aatactccaa agaaaccttc atgatttcag tttttgtata ttattatacc ttaagtatat
     2581 cactttaatt tatgtaaagt caattaaagt taacgctttt tattatactc ttcattcttg
     2641 tatttttcat atagttcttt aactaattgc ttctcataat cagtcaaatc gccctccaca
     2701 aactcaacat taaattgttc agctaatccc tttactatgt ttttctgtag aatatctatg
     2761 tctatttttc tgccaatttc attttctatt ccacttgctt ttttagctgt aaatttcata
     2821 gctctttctt tcaattctgg tgtcttagtt tttattaatt taaacaattt ttctacatca
     2881 aaatccagta ttattgaacc atgttgtaat aaaacaccat cttttctgct ttgagcactt
     2941 ccaattacct tcttattatt aatagtaact tcatatgatg catgtgcatt aaaacatgca
     3001 gctgataaat tttccctact tattctctcg cctctattta aattatctgt ttctatacca
     3061 cttagattca atcctttaac tagaccttca cttatatatc tataagatag gttgatgctc
     3121 ttatcgatta aaggattatc ttctcctata attatactat aagttagctc attatcatgt
     3181 aatacagctc ttccacctgt aattcttcta gtatagtcta tattcataca tctacactta
     3241 tctaaatcta tctcatcttc caatttctga aaatatccta tactcaaaca agctggttcc
     3301 catgtataaa atctaagagt tggcttatta tgtcctttct tatatgcagt aaatatagct
     3361 tcgtcaattg ccatattcat tgctccttca tatgatttat tatgtataac tctccactga
     3421 ttcatgatta ttacgccccc atttttagta taaatatatc ctagaaatcc tctatagatt
     3481 tatttctctt attgctaaat ctatctcact ttgatttaaa ggtcttgaat agttgtatat
     3541 agtacttccg ggtctttcat tatagtatgg tctattacaa tccttacaac ctcttatttc
     3601 aaaaggatag cctttgttta tgtcttttaa aatatcattg tcaagcttta tactatttaa
     3661 gtatccattt ttaaacttaa aatactcttt tggataacct ttatttatca tataagacat
     3721 taactgtacc cttctataac tttctatgct tggttgattt atcttttcca ttttagttcc
     3781 tctaactgga gtaaatgcaa ataaactaat cgttacatca ttttccttta aataaatata
     3841 taaattgtat atatcttcat gactttcacc cataccaact attatatgtg tacttatttt
     3901 attgggatat gattttgaca tttcagttat aaattttaac ttttcatcat agttatttcc
     3961 ttttattttt tcatatagtt ctttatttga tgcatctatt gctattccta ttttatcaac
     4021 tcctactgaa aaaaacttct ttatctgttc atcattttct aattttgcag atagtgaaat
     4081 tggcatgtct atcttaccac ttatatagtt tataaagtct aaaacagagt tatgtgcttc
     4141 ttcacttgcc attgattgaa tacatattct ctttatattt tttcctttat aagcttttaa
     4201 agcatctaat atttcttctt ttgaaaactc tggccaaatt actcttgata atttatcttt
     4261 tctagtacta ctttctattg attgagaaca aaaactgcat ttatttatac atttttctcc
     4321 aatcattata tatgctgtgg taggaggtat atcgcttttt ttgtttaata tcccaagctc
     4381 tattgctgtc ccaactgata gtctaatcat aaaacgcaac actccttaca ctcatttatt
     4441 gtcatattca tttctatagc cttattcttt gcagaccttg atggtaatac tattctattg
     4501 actccacaca ttatagataa ttggtctagc tcttttctat acactcctct tggtctcata
     4561 caacctagat taatttcagt atctggtaaa ttaattctag cttcacataa aatatctgct
     4621 accccttcta actcaggagg ttctacattt tcatactcag tcccttttgt tggaataagg
     4681 actataaatg taagcttatt aggaggattt ttctgtaggt attctataat ttcatattca
     4741 ccctttattt gtcctccttt taaaccaata catatatgtg gtgctacttc tgtatgttct
     4801 tgtattgtat tataaacttc aatataatct tctttactct ttttcatctt ataaacttct
     4861 cttattgtct catcatcaaa cactaaatca aaagaaacta tatctaaata cttacacaac
     4921 tctacaatgc tatctttatc cataagacct aaatgagcat tcaatctata tcctttctct
     4981 tttaagtttt taatggtatt aatatgagta tttataggga catctccctc ataactacaa
     5041 ccgccactca atagaaagct agttatattc cttgattgta ctttttcctc atattcttct
     5101 attggcacca tattgtttaa gtagtgaccg ttgcaatggg cacatttcaa actacattga
     5161 ttgcttgtag tactaagagc tagtgtctgg ttaggataag caaactctat tttatttcca
     5221 aaattcttta atttaatatc aaatgctatt ttcatttttt cagatagatt cttattcata
     5281 gtttattcct tcttaagtac attttactct tctagtaatt tctttgctgt tgggaactgc
     5341 tctatattcg tatgtggtaa gaaacatgca gaaataaatt catccatata acttggatat
     5401 acactaagtt caacataagt tatttgatta cctatttctt caagtttatt ttttgcatct
     5461 ttacttatga gagctaagta agaaccaaca agtgaactat tccctatata agtaaatttt
     5521 tctctatcaa tgtcaggaag taaccctata attattgagt tttctatgtc taggttgttt
     5581 cctataccac ctgctatata aaccctatct aatatactaa aatccatacc taaactatct
     5641 acaagagtgt atgcacctga atatatagct cctttagctc tgatgaaatt atctatatca
     5701 acttcattga ctactatatc attttctaaa tcaaattctt ctttaaatgc taatacatac
     5761 tctcctatct catgctcatt aaatctaact ctcttattat ctaaatccct gtatatttta
     5821 cctcttctat ctattacacc tttagtaatc atttgacaga ttaaatcaat tataccagaa
     5881 ccacatatac caactggtgc acattcatca ataatcttta atgttggctc taaagtttct
     5941 ttatcaataa ttactttctc tattgctcct gctgatgccc tcattccaca gcttatccct
     6001 ccaccttcaa aagcaggtcc tgctgaacat gcacagctca tcatataatc tttatttcca
     6061 aatacaatct ctccattagt tcctaaatct ataaataaaa cattttcttc acttgaccat
     6121 atccctgctg ataatacacc tgctgttata tctcctccta cataacttgc aacagatgga
     6181 gctaagtaca catatgcact gtcatttaca aaaagaccaa cattttcacc cataagtttt
     6241 ggagatttta aaaatggtgg tatgtaaggt tcttgtctta agaagtctgt atacacaccc
     6301 aagaataaac ttgtcatagt tgtattcccg gcagccacta atgtaactac atcttcctta
     6361 tctattccat ttctttcata tattgttttt aatagtggat ttatagtttc ctcaactata
     6421 gctttatgaa gtgtctctaa tccatttttc ttagttgaat atataattct atttataaca
     6481 tctgctccat attttatctg tgcatttcca gaagatgctt tatctactac ctcttttgaa
     6541 tataggtcta ctaaacaaac aactactgaa gttgtaccaa tatctattgc aactccgtat
     6601 aaggaatttt ctttattacc ttgttctata tttatgattg taagtttctt ttgtttttga
     6661 acatatgtta ttgtaacctt aaaatcactc tttctaaata cagttggcat ttttctaagt
     6721 atatccaatc taaaatctat ttcattatat cctaggctat ttctaacata tctttccaat
     6781 ctatctacat cacttatatt atcatctaaa tttggttctt ccatttctat gtatttcttt
     6841 tttatatttg ttttaaattg aaggttatgt tcttcaatta tttttttagc tctttcaaaa
     6901 atttccctat cctctttttt attactacct tctatcttca taccatgcat agaagatgat
     6961 actttagaag gaacttcaat ttctatatct gatattacct ttgtacaaca agctagtata
     7021 taaccttgct cccactcatc atctgttata tgtcttgttt tttctgtatc aacttttcca
     7081 ttcaataatt ttactttaca cttcccacaa gacacgtttc cattacaagg agcatctatg
     7141 aatatatctg catttcttgc aacctctagt aatatatcac cctcattaca ataaacctct
     7201 ttattgtttg gtgtaaaact cacttttatc atgttatccc tcccaatttg ttctatatat
     7261 gataaaagga aagtatagct aaatttgcgt gcactcaaat tttactatac tttccccaat
     7321 tataataata tcttaatctt taattattct aatccagcct cgtattcttt atcacttaat
     7381 aacgtatcaa gttcagccac atcagctagt tttactttta atatccagtt ttcatatggg
     7441 tcttcattta tacattctgg ctcatcttct aatttttcat taacttccaa tacctcacca
     7501 ttaacaggag atattaaatc agaagctact ttagaagact caactactcc aaaatctact
     7561 cccttagtta cagtatcttc tacttctggt gtttcaacaa acaatatttc tcctaattga
     7621 tcttgagcat agtctgttat acctatatat acaacatctc catcaattac ttttacccac
     7681 tcatgatctt tagagtattt taattctggt aataatttca tttcgataga cccccaatat
     7741 tatattattg attaaattac ttaaatcata ttaatattat atcatattat tttaaatttt
     7801 ccatcaatta cattacactt tccatagcta atgctggatg acctttttct tctaagaaag
     7861 ctaatactcc ttcagaatcg tcagctatag tttcatcaca aaccatatca cagaagtttt
     7921 ctattccagt catttcttta actgtagcat ttaatttatc agctacataa tcttttaatt
     7981 cttttggcat ccaaactatt ctttctggtc caccttctgc ataagcgaat tttttagaag
     8041 atatgaaatg tcttccgtgt cccataaatc ctggagtttg aactcctcca cctgtcatag
     8101 aagcaagttc tccgaaagtc ataccaactg gagtaacact tgcaaattct ctgttaacta
     8161 caacaaatcc gtttgcttct ggcattatac cacagataca ttcgaagcat ccacaagaag
     8221 tcattgggtc ttctagtata gaatataatg taacagattc aactgcacct tgagatattt
     8281 ggtttacagt ttcatttact gaattccata cacctgttct atcatctaag cactcgcctt
     8341 tttctattgg ttgacaagga cctgttgggt ctaattcttt agtagcctta gcatctaacc
     8401 agctaactgc tccacaaaga ccaagtcttt caggagtaac aacacaaacg tgagctggtg
     8461 caaatgattg acataagtta caagaataga aagtatcaac actttcatca actaatgaag
     8521 ctaatctttc atctctagca ttgtacttag ctattgcttc tccttttaat tcagacactt
     8581 tttcagcatc tgtaattata gttatttcac acttatcaac tactgattca aattcatcta
     8641 acatcttagc atataaaact tctcctatat gctcaagtct aaatccttta tcaaatgcat
     8701 ctttagagat tcttacccaa gccatatctc tttgtccaac gtgcattaca ccttctatat
     8761 agttcaagaa gtagtggaaa cgtctttcaa gaactggctc gaaatcttct tgcatatttt
     8821 taccagctat tttaactatt acagcaagcg gtaatcttaa tacaccatct gcatcaactt
     8881 catctatatt tggtcctatt atttctatct tatggtcttc aacttctgtt gcttctttct
     8941 taacaacaag ctcccaagct tcagttctgt ttccaccaaa ctcagcaaac atatcgctct
     9001 ttctaattct ctcaccttca aatgctgctg caaatgaaac tgggataggt atttcagtaa
     9061 cttttatttt tattcctcta gcttctaatg aagtagctac tatcttatcg taatcttttt
     9121 gagttaatag gttcattgga acttcaagaa ctgtttggtc agttataact gggaatccta
     9181 aagctattgc tccagcacct gcagatacaa ctaattcact taatggtcca aatgcattaa
     9241 caaatgcagg aactctatta gctgtgtact ctaataatcc gtttaaatca ccaggagtta
     9301 atccaccgaa tattaatgct gctcttactg caacagatac aacgtgtata acagaagtta
     9361 catcatatcc tagaggtata actcttagct caagacccat ttttactcca gcttctatag
     9421 cttggtctat aactttacca actaagaaag ttagtaaacc tttagattgg taatctttta
     9481 taacttttgc tgctgattct gcatctggac actcaccaag aacaactgca acaccaggta
     9541 tatctcctgt aactaatggt acaccaagtg atctgattat agggtcagtt atatgacctg
     9601 cacatggctc actatatgga gcatccatag ttgaatattt taatgcttca attacttctg
     9661 cagctaaagc cgtagctaaa ccagcattaa aagcatgttc tagtaaatga gttctattta
     9721 ttaaagattt tactacttct aaagcacctt ctaagtcccc taaagtgttc atcttttgtc
     9781 ctgttgcagc atatatacaa ggtaatgaat atgctgtatc agggaaagca actttatgct
     9841 cttttccatt cttttctata gcttcagcta acatagcttg agccgcacct aaagcttgtt
     9901 ctgaccctgt aaagattata ttatatagat tcattctatt tcccccttta atatttacac
     9961 ctatttttta ttaactatac cctaaaaacc ctagaaagca aatccacctt catttaactc
    10021 taagaaagag tctgcatata aagatgcccc gttgattatg tcacaagatt ttacagtttt
    10081 cattaattct gggtcacatg gattcattat agctgaacta aatccattag ccattgccat
    10141 tgctaagaat gcactatcta aaacaggtct aacatgctta ggacatccat tagatacgtt
    10201 agataatcct cctgtagtta aaagtcccat ctcagtcatc atttttatag cttctaaaac
    10261 ttctacttgt ttttcttgca taccttttat aactaagcat aatgggtcaa atagtatatc
    10321 agttggctcc attcctaaca ttaagccttt ttctaatatt tcttggcagt aagccatacg
    10381 ctcatcatta tctcttggga taccttcttt tgcacataaa cctataacca ttgcttcgta
    10441 ttcagctgct atatctatta aatcaaatct tgagccagca tcagcagagt ttattattgg
    10501 ttttgcatta gttctatcat aaactttaag tccagcttcg atagcttttt tattagctgt
    10561 atctaatgct ataggaacat tgttgaactc agattgaagt aatttgattc cccatgtcat
    10621 tatttcttcg ccatctcttt cggcaggtcc tatattaaaa tctatatagt gtgctcctgc
    10681 ttctaattgc tcttttgctc tttttaagat tggagctggg tctctttctg ctaatgcttt
    10741 tcttattgaa ggtgatatac aatgtattct ctcacctata atcataaatt tttccattta
    10801 taatgtcccc ctttaaattt aatattaata gttagttaat attttattag tttgtttggc
    10861 atgctttttc ttggtatcct tttaagaact taggtaattc catagattcc tctggtccta
    10921 taactacatt ccatcctggt aagttatctt ctatatcacc tttaagtact gcaacttttc
    10981 ctggaatgat aaggtcttta caattagtta cttcttctac cttactttct ttaatgaaag
    11041 cagagattga gtttccacca aatttaccag cagcccaaga tgtaagaact gaatatccac
    11101 ctgcatctgg tattactaac catactggaa cttttgatct ttctatatca ccagcaacta
    11161 tgaagtaagt taatgcaaag tcaacagtaa ctaatactgg agaattttca tctgggttgt
    11221 tgattggata aatctttggt tcaactctca ttggtctttg tgggtcagtg tatatgtttt
    11281 gtcttagtgc aaataatggt aatgccttag cataactaat atcatctatt actattatag
    11341 aaccgtattt tatagtgaat attgatgata aagcaacttc catcataggg tttgaattag
    11401 ataatctatt tgcaaatact attgaaggat atccaaatgt tctatcttgc tcttttaaag
    11461 ctgttcttct aacttgtata gcattagtgt aagtatcttt tatgttttct cctgttacat
    11521 ctaatactaa ttctttatat ccagcggctt gaattaattc aactgtttca tataattctt
    11581 ctaagctacc agcttttaca cctaatggta aacttgctcc tttaactact tctatcatat
    11641 ctttgtagtt ctctttagtt gctccatata ccattggtct agcatctttt aatacttcaa
    11701 cagcttcttt aactacttgt gcatcatcac aagctaaaat ataagctact tctaatccac
    11761 tgccttttat tttatctatt aattttaaat acgcatcttt atctccacaa tactctaaaa
    11821 cagccatttc aactttcatt tgttcaccaa ttcttacata gtcaactttt ttcatgttag
    11881 ctattttagc atctactgct tcatcactca tacaagtaca gaatgaaact gcatatctat
    11941 ttctacttac taatgttttt tcatgtctaa ataatactgt ttctccacct agttcatact
    12001 cagatgcacc agcaccaact tttaaagctt tcattaaagg tgcagtagcc tctgataatt
    12061 ttgctatagc gtcatcagac atatgtgggc atttacctac ttctacagct cctgaagcaa
    12121 ccttcataga gaaagccata caagttggga atccacaatc cttacagttt ttctttggtg
    12181 ttaatttaaa tatatctaaa gcttttaatg ccattatttt ttccccctaa ccttaacaat
    12241 ttaattaagc taatgcgtta accaattctt ttatagtttc tattgatttt ggatgacgaa
    12301 gtataactgc gtttgaacca cctactaaaa cacttgctgc agttgaaact tccattgcta
    12361 tagttctttc ttctggacaa ccccaatctg gctcatcttc tattggagct atagcttctt
    12421 taacatgacc tacttcaaaa gctactggtg ttatgatagg catttgtaat gtcttatcat
    12481 tttgaccaaa tgctgcaagt ctaattctat ccatagtaga tgcaacgtac tcatatccat
    12541 aaccaactgc tgaacatcca acattcataa ctatattttc acctttaacg cctagttgag
    12601 ttaacaatac gtttaattgt ttagctaggt ttatatcaac agaagattca gctcctactt
    12661 tatgtgcata agccatacca gctgatgctc ctactccttt gtagttatct tctactgctg
    12721 acataaataa gcagttatgt ccatccaatg tttgagctaa tttttcaaat aatttaccat
    12781 ctttttcatg atttcctgaa ccagctacaa ctaaaggtaa ttttattgct tcaattactg
    12841 ccttagctac atcagcacat tcatcaactg acttatctaa tccatttggg tcagaaccat
    12901 caaattttaa acaaataaaa tctgcttgtg tattagcttc aacatattta gcccactcta
    12961 ctggacaatt agctacatct ttgtataatt ccttatatga atcagtccag ctttcaggat
    13021 aaacgtctga tatttgtata cctatttttg gagaattacc tacttctcca tcaaaactat
    13081 aaaaaggtaa tacattttcc ccacctaatt taattgcttt ttcccctatt cctacttcaa
    13141 cttctgatat ttttccagaa tatttttgag tagacatttt aaatgccata tttttttccc
    13201 ccttaagcct attttaattc taatttagca aaaatttctt tcatagccac tttagatttt
    13261 gaatcttcag gtaaattaac taaaggaata cctgtagaat catattcata tatcaattca
    13321 tccataggta caacaccgat tagctctagg ttatgctttt caatttcttc tttaacacca
    13381 tcattcataa ccccatttgg aactttattt acaatcaaaa gtattcttcc aacacttaac
    13441 tttagttcct cagctaaatc tcttattcta gcaacagctt gtatgctacg tcttgagcaa
    13501 tcactaacca ataaaagtgt atcaacatgt ctagtgactt ttctacttag atgttccata
    13561 ccagcttcgt tgtccatgac taagtatttg taatgaccag atattttgtt tacttgttct
    13621 ctaagtatcc catttacaaa acagtaacat ccttcacctt ctgacctacc cattactaat
    13681 aagtcatatc cttcgccttc ttcgattata gaatttaatc taaattgaag atattgtgcc
    13741 tttgtcatac caccagggaa tgcattgcct aacttttctc tttgatttac ttcttctctt
    13801 atttctccta ttgttgcttc aacttcgata cctaacactt catttatgtt agcattggca
    13861 tcagcatcta caactaatac tggtcccttt ttgtccttaa ccaaataatc aattaaaagc
    13921 cctgtgagac ttgttttacc agttccacct tttcctgcaa cagctatatt gtatcccatt
    13981 ttaagacttc ccccttaaat ctagccacag agccttatgt actctgtctg tctattataa
    14041 tttaggtaca gaatgaacac attctccatg tacatcatga agtgcttcca ttagcccttc
    14101 agataaactt ggatgtggat gtattgcatc ccctacttgt tctactgtta atcctagatg
    14161 aacagcaagt gacaactctg tcaataaatc tgtagcatga ggtcctacta ctgctgcacc
    14221 tattatctta tcagtttctt tgtcagctat aacttttaca aatccttgga aatgtcctat
    14281 agcttgtgct tttccaagac ctctaaaatc aaattgacct acattgtatt ctacaccttc
    14341 agcttcaagt tgcttttcag tcttaccaac accagcaact tcaggttcag tataaacaca
    14401 tcttggaatt gctctataat ctactacctt agtcttacct aaagcatttt caactgctac
    14461 gataccttct ttagaagcaa catgtgctag gaaaggagta tctattatat cgcctattgc
    14521 gtatatacct tctacattag tttcaagatg ttcattaact actacttttc ctctttccat
    14581 ttcaattcct atatcttcta ctccagaatt gtcaaggtta ggtcttctac caacacatac
    14641 taatgcatat tgtgcttcta taacctttcc attagaaaga gttgcaaccg ccttaccatc
    14701 aactacttca caagtttgta ctccaatacc tgtaataacc ttaattttat ctttcttaaa
    14761 ttgtctaagt agttgtttag ctacatcttt gtcttcattt aatagtattt ggtcaaccat
    14821 ctcaactata gttacttctg ttcctaaggc tctaaagaac tgccctatct cacatcctat
    14881 aactccacca cctactatta acatagactc aggtatctct tcaagtccaa gaacttcatc
    14941 actagttatt acaacttttc catcatatgg gaacattctt ggtacaactg gaacagaacc
    15001 atttgctagt attatcttat ctgcttttat agtttctact gttccatcat ctttagttac
    15061 ttctatagtg tttttatcaa ttaattttcc aaaaccatta actaagttta ctcctctttt
    15121 ttcaaataag aactctatac cacttattaa ttggtttaca actttatttt ttctttccat
    15181 tatggcagtg aagtttggct taactgtacc atctatttct ataccaaaat cctttgcttc
    15241 ttttacagta ttcaatactc ctgaagaagc aagaagtgct ttagttggta tacatcctgc
    15301 atttaagcaa gttcctccaa ctcttctttt ttcaataacc gttacatctg caccaagcat
    15361 agaagctttt atagctgcta catatcctcc tggtccacca cctactacta ctatcttcat
    15421 ccttaattcc cccaaaccta atataatcta caatcttgca atactataaa cctgcggcat
    15481 ccagtatagc aggtatattc tcttctgccc caattgccat tatatgaaca ccatcacata
    15541 aatcttcttc ttttaattgt ttgataagtt cccctgccat ttttattcct tcacttaccc
    15601 agttttcttt tccagctgct cttaatcttt cgatttgttc atctggaacg aatattcctg
    15661 gtacatttgc agtcataaat ttagccattc ctggtgattt taaaggtact attcctgcta
    15721 ataccttgca atccatatcc cttgttaatt ctctaaactt tctcattgta tttatgtcat
    15781 aaagtgcttg tgtttggaaa aatttagccc ctgcttttat cttcttttgc atttttaata
    15841 attgaacttc tattggagaa tattctggag ttactgatgc tcccagataa aaatctggag
    15901 aaccttttaa cttattacca gccatgtccg tacctgccat taaagtctca gcagtttgaa
    15961 gtatccccac agaatctaaa tcaaacactc ccttagcctg tggatggtct cctacgctag
    16021 tatggtctcc tgttagtgcc aacaaattgt ttatcccaaa tacacctgct gacaacattt
    16081 ctccttgtat agctattcta tttctatctc tccctgttat ttgaataact ggctctaatc
    16141 cagcatcttt taataattta catgtagcaa gagaagttgc tctcataact gccgattgaa
    16201 agtctgttac atttgctgca tgaactcttc caactaacgg ctttgcacat tctattaaat
    16261 gtgaaaggtc agtccctttt ggaggagcca tttcagtagt aactgcaaat tttccacttt
    16321 ctaatgtttc tcttaataag ctcatctcta atcccccttt taaccttcta aaaaaaactt
    16381 ttatgaattt gctgctgctt ctttcttttt ctttgcactt aaacttcttg gattattttg
    16441 cttagaatag tcttttggtg gtcttatttc tgctaagtta tctaattgtc ctatagcttc
    16501 tagtctttca tatatcttta tccaagcaca atcattctct gagtttactt cacacttacc
    16561 atttttagct ccaccacaag ccccattcat aagaccttta gcacacatag ttactggaca
    16621 tatcccacct gtccaaccaa gttcacagtc tccacaagcc ttacaggctt cttcatattc
    16681 tccaactctt tgtacttccc ctataaacaa agtattgttg gctggataaa ctggcttatc
    16741 ttttaaattc ttaactattg tctgtgtacc atctccacat gctaaagata aaactgcatc
    16801 agcttcctta gtctcttctt ttagagcctt taaatccttt ttagacttta aaaggttaca
    16861 agcagggtca agcattacat atcctaagat ttttttgcct tcaccttcaa gagtttcctt
    16921 catttttaaa acttcttctt ccccaccact tttacaagtt gctgcacatt gattacaacc
    16981 aactaaaacc agcttgtcaa aatcctttaa gtaccctagc acttcttcta atggtttatt
    17041 ttcagaaatt atcatatcta cttcccccct aaaccttcca catatatatc tctatttatt
    17101 gttttgtaat ttacaagcct taactacatg ttttgcaagt atagatgttg taactgaacc
    17161 aacccccgct ggaactggag ttatcattga tactttgtct aaaactgcat ttgtatcaac
    17221 atctccacat aaatttcctt cttcatcaac atttatacca acgtctataa ctaccgctcc
    17281 atctttaaca aaactttcat caaccatctt agctctacca atagctgcta ttagcacgtc
    17341 tgcctctgct gctacacctg atagattttt agtttttgaa tgacatattg taacagttgc
    17401 atgttcactt aataaaagca ttgatactgg ctttccaact accattgatc ttccaagaac
    17461 tgttacctta cttcctttta aatcaacatt atagtgtttt aatatctcaa ctactgctgt
    17521 aggtgtacat ggtggaaatc cacttttatc cccttccatt accttagcag agtttattgg
    17581 actaaaacaa tctacgtctt tttccggtgc aataacatat tttataactt cttcatttaa
    17641 gtgttttgga agaggtctaa aacataatat accattaaca tttttgtcat cattaactct
    17701 ctttaataca tcaatatatt cctcttgtgt tatatcctca gctaactcta acacttctgt
    17761 tgttatacct atgttttgac atctctttag tgctcctctt tcataagata aatcatcact
    17821 tcttgcccca actctaacta ttgttagttt aggatttata ccttctttaa ctagtaaatc
    17881 gacttctttt attaattctt cactaatttt atcagctaca ggttttcctt ttataatttg
    17941 tcctttagtt gacattcctt ccatacattc acaactcccc tacaagcaaa cttaaattaa
    18001 tttattctat ataccacata ttaataacat agtatctact ttcgtcattt aaaaattatt
    18061 ttccaagagc tttttcaact ttggcataaa cttcatcaca aatttttaca ccatcttcaa
    18121 ctaacttatc tacttctatt tttaccttat taacatattc tttatcttgt atagaattta
    18181 tatttataac tacatttaat tgtccaccaa gtatagcagc tcttaaacac tgaactccta
    18241 caccaacatc acttatagca agtcttgaac ctttatcaac taaatcttca tgtaacttaa
    18301 tagcttcata gcaaactctt actatgttta atggtacttc acaagcaact tttaatgcct
    18361 tttccattgt ttctgctttt attctttttt cttcttctgt ttctttagga agcccgtaag
    18421 cttttgataa tggtaagaaa cattctgcat catcatctat catttttaat aaatcttttt
    18481 ctaactttcc tgccttactt aatatctctt ttacgctttc ttcatactca gcatacttct
    18541 tcttccctat tgtaaggtta cataccatac tacctaaagc catacctatt gcacctacta
    18601 gtgcagcagc tcccccacca cctggtactg cttctttgga agatagtact tctacaaaat
    18661 caacacaagt tttatctgct attttcatga taatcctcct tgaaaggatg taggaaaata
    18721 tctcaaatta ttcgagatat tttacgaact attttaagat atctcctact ctttatttat
    18781 aaattaattt ccttagaata atccagctat tactccattt tcatcaacat ctattttttc
    18841 tgctgctgga acttttggta atcctggcat cttcattatt tctccagtaa gtgcaactat
    18901 aaatccagct cctgctgaaa ttgttgcttg tcttacagtt attctaaatc ctgttggtct
    18961 acctaattta gtttggtcat cagttaaaga atattgagtc ttagccatac atactggaac
    19021 tttagtaaat ccaagttttt ctaagttagc tatttcctta tcagcttcag gagtataatc
    19081 tacaccatct gctccataaa tcttagtagc tatagcattt atcttgtctt ttatagataa
    19141 atcatcttca tagcagaatc taaagttatt ttcttcttca tccaataatc ttaaaacttc
    19201 tttagctact gcaattccac cttctccacc ttttgcccat acttcagaaa gagcaacatt
    19261 aacacctaat tccttacact tgtcttcaac aagtttaagt tcagcttcag tatcaagtgg
    19321 gaatctattg attgcaacaa ctgctggtaa tccatatact tgagttatgt tttcaacatg
    19381 ttttaataag tttggaagtc ctttttcaag agcttctagg ttttcgttat ttaagtctgc
    19441 tttagcaact ccaccattgt attttaatgc tcttacagta gcaactatta taacagcatc
    19501 tggttttaag tttgccattc tacattttat atctaagaat ttttctgctc caaggtcagc
    19561 accgaaacca gcctcagtta ttacatagtc tgcaaagtgc atagccattc ttgtagctat
    19621 aacagagtta catccatgag ctatatttgc gaatggtcca ccgtgtacaa atgatggtgt
    19681 tccttcaaga gtttgaacta ggtttggttt taaagcatct tttaataatg cagccatagc
    19741 accattagct tttaattgtc tagctgttac aggttcacct tcatagctgt atccaactat
    19801 tatgtttcct aatctttctt ttaaatcaga tatatcactt gccaaacaga acgctgccat
    19861 tatttcagaa gcaacagtta tatcgaatcc atcttgtcta actgctccat cacctttttt
    19921 acccatacca tcaacgatat ttctaagttg tctatcgttc atgtccacac atcttctcca
    19981 agttattttc ttagggtcta ttctaagtgc atttccttgg tgaatatggt tatcaagcat
    20041 agcagctaaa agattgttag cagctcctat agcgtgaaaa tctcctgtga agtgtaagtt
    20101 tatatcttcc ataggaacaa cttgtgcata tcctccacca gctgctccac ctttaacacc
    20161 aaatactggt cctagagatg gttctcttaa agcaactaat acatttttgt ctagtttagc
    20221 aaatgcatca gcaacaccta tagtagtagt agtttttcct tcacctgctg gagttggatt
    20281 tatagctgta gttagtatta acttagcttt tttaccagtc tctcttttta gtagattgta
    20341 gtcaactttt gctttgtatt taccataaag ttctacatca tcttcaccta gtcctaattt
    20401 ttttgcaacc tctctaatat cttgaggctt agcttcttga gctatttcaa tgtcagattt
    20461 aaatcccata actgaatctc ctcccacaac ctaaatagat ttaaaatata atttttgtaa
    20521 aaattgtatt aatatgtaaa aattatattt tataagataa ttatacatgt aaaattactt
    20581 aaaaactagc tactaagcta ttaaaatatt caaacttaat agctagttat ttatctaatt
    20641 acttacttat tacctatcta ttgcctatgt ctaaccttta accaacttaa aatcccaaat
    20701 caaaatttct atctatttta tttatcctta agtgacatta atttatcttt tattgcttta
    20761 atctgttctt tcgttgtttc actagaatca tatggagatt gattagctct atcagaatca
    20821 ataacatctt ggttatagta aataaatcct aaagattctc catcttctaa gttttggatt
    20881 ataaattctt catcttcctt atttctaatt ttatttccta ctacaaatac tttatttaca
    20941 cctatatcat gtccaagttt cttaactttt ctataagtct gtagacttct ctctcctggt
    21001 tctacaacta ctataaaagc atctacacct tgggctgttc ctcttcctaa gtgttcaatt
    21061 cccgcttcca tatccataac cactacatct ttattttgta gtattaaatg tgagcagagt
    21121 ctcttcaaaa gaacatgctc tggacataca catccagtac caccagaatc tacggttccc
    21181 aatgtaagta atcttactcc attgtattct ttacagaaat tttcagggat atcgtctact
    21241 ttaggattca tcttaaacat ttttccaaat gaacctacag aagcagctgt tctatctgaa
    21301 actaattttt tcatttctga tataggtact attgattcat atacttcttt tggaaaacct
    21361 agtgctaaag ctaaattagc atcagggtcg gcatcaacag caacaactct gtatccgtct
    21421 tcagcaaaca tcctagagag cattgaagaa aaagtcgttt tacctactcc acctttacct
    21481 gttattgcta tcttcataat ccctaccacc ttttattact atttgctttt tagtagttaa
    21541 ttttacagtt aaatattttt tatactctat atttttatac actaaaaata aactacagct
    21601 agtccttaca ggaaccaatt aaatttaccc tactagcttt tcaaagcagc atgtatagtt
    21661 ttattattct ttaatattgt ttatatttta tttattcagt atattcatta tatttcgatg
    21721 ttctttactt taatatttga ctttattcaa ctttactcaa ccagaaactt cttataaaaa
    21781 cgtttattct ataacttcac tattttttaa gcttcagcct tttctgccat tttttcttca
    21841 actaatgctt caaagtgaac acgttttttc tctatatctt ctattattgt agctgctaac
    21901 tcttttggat ttgtatttac agtaaatgta gctccaactt tatcttttaa ctcactagtt
    21961 aatatatcca ctgctcttga agaaccagct attggaggca ttattcctaa ataagtgtct
    22021 ataccagaag ctacaacata gcatcctata gcaactgctt tttctgacat ccattctgga
    22081 gctataccta caactggaag gtcacacata tccatatcta agtaattagc tgctgctcca
    22141 actaaatcta gtatacgact tatatcaaca caagaaccca tatgtagtac tggtgggatt
    22201 cctactaatt tacagacagt tgcaagacct ttacctgcat atttttcagc cgcatcagat
    22261 tccattaaac caaacttagc tgctgctgaa gctccacaac ctgtagtaac tactattatg
    22321 tcgtttttga ttaattcttt cattattgtt acgtgagcct catttgaaac tcctttagca
    22381 ttgttacatc caactacacc agctgctcct cttaatactc cagcttttag acagtctgtt
    22441 aatggcttaa ctgttcctgc tgggtctata tgagagttta caactctgtc taattgattt
    22501 actactgctt ctacactata cccaactgtt gcgcctgatt ttaattcagg tataagtact
    22561 tttgatttat ctctattttt aaagtttaaa atagcttctt taactattgc tttagcatca
    22621 tcaagtgctt gttcttctct aaattcaata taagttgaac ctgttatctt agcttttgga
    22681 gaagttgtta caaatttagt atggtaacaa tctgcaactc tagctaatgc agggaatata
    22741 cattgaacgt caactattac agcttcaact gctccagtta ttattgcaag ttcttgttga
    22801 tggaagtctc ctgctatttt tacaccatgt ctcattgtaa tctcattacc tgtacaacac
    22861 atacctgcta agtttattcc atcagcacca acttctttag ctaaagctac taagtctggc
    22921 tcttctgatg ctaaaactat catttctgat aatgctggct catgtccatg taatattatg
    22981 ttaactttat tttcttctaa tactgctaaa ttagcctctg ttcttcttgg cactggagtt
    23041 ccaaataaga tatcgcttag acgagtacct atcatagaac caccccaacc atcagccata
    23101 gaagttctaa gactcatgtg tattaaacta tctatatcac cagtacatcc tatatgagta
    23161 gagtgcatta ttgttgcaat ttctctatct atagctcttg gctctatagc gtattctttc
    23221 catatttttt gtcttggttc tggtgcattt tttataaatc ttaatgtccc aaaaggtttt
    23281 ccaaattcca ttaatgcaat ttctgccact tcatgtgcta catcatagat atctctacct
    23341 tctgtctcta catcccattc tttagctaaa gtaattaact tagcttcgtc tcttatttta
    23401 tagtttccat ttggatcagc taatgctaat gtatgtgcta tatctctagc atggtctgaa
    23461 tgggcagctg tcccaccagc aaccattctt gcaaagtttc tacctactat agtatgctca
    23521 tctgctccac ataaccccct tggagtctta gggcttattc tacatggtcc cattccacat
    23581 attctacagc aaacaccttg aagaccaaaa ccacattgtg ccttaaagcc ttcttttcta
    23641 tcatacattg tttctacgcc ttcttctcta gctttagcta tcatcttctg gctgtttaaa
    23701 tcaattgtta acattttttc atccatattc ctatcccctt tttttgattt ttaaaatccc
    23761 ccaggtgact gacttcatgt cataaaataa taaaataaat gctttctaag ttgattatat
    23821 actaattttc agtgctttgc tttagattta aatatttttt taacaaaatt agataataca
    23881 taaaaatttt attttctaca cttaatttaa gttgatttta tcgcttgttt ttggctattt
    23941 gttaatatct aaacctactt ttttgatact tcagtttaat taaattatgt tttgattttt
    24001 ttaaatcaaa tttgaaattt ttctagtcat ttttatatta ttgcagattg tttgtgtatt
    24061 tattatcttt cactactgtt tggacttatt acagattttt actccctaaa cttaattagt
    24121 aaaaacttct tagaatagca ttttttggac taattttttt gaattttttt atttttccta
    24181 ctttaatatt aattatagct tcaaattgaa catttgtata tattcatttg aagctataat
    24241 tattttttat ataaaacttt tttaatacag cattaattta gacattttga ctattttttc
    24301 tactttaatt catttatttc aagcgattta tcctatttca tatttttagc tacaatagcc
    24361 ccatatgcaa tagaatataa tttagattca tgtgaatcag ctcttgacac taaaactata
    24421 ggagcttttg cacccataat tatacctgct gatttagagt ctgccatata tgtaaaggct
    24481 ttccctattc cattgccaac ctctattgtt ggaaccaata tgatatctac atctccagat
    24541 acttcacttt taaacccttt tatctcacta gcttcttttg aaacagctaa atcaaaagcc
    24601 agaggacctt ctacaattac attttcacca aattctcctt ctttacaagc ttttgctaac
    24661 atatctgcat ctacagttgc ttgcatctta ggatttactt tctctttagc tgccaaacat
    24721 gcaactttta cagtttccat tcctaaagct tttgcagctt ttatagaatt ttttaatatc
    24781 tttgcctttt gttcataatc aggagctata ttcatacctc catctgtagt tataagtaat
    24841 ttatggtatt tttctaactc atataccata acatgactta ataaactatc agttctaaga
    24901 ccatagtctt tatttaacac ttcttttaat atgacagatg tatctaataa tcctttcatt
    24961 acaaagtcgg cttctttact tgatactaat ttaactgcta tttctgcaca ctctttgata
    25021 tcatccttat ccactatttt gataccactt aaatcaaatc ctatttcttt tgatatttct
    25081 cttattttgc cttcatttcc aaccaatata ggagtaatta tatttctttc tacagcatcc
    25141 tttattgcca ttaaaacttc tttatcatga gctgctgcta cagagagtac aattttctct
    25201 cctcccttaa gttgttctaa aatatcatct aattttttta tcaaaagtaa cccctccctt
    25261 atatatattt aactaaataa taccatattt gctatacctt ttctcaattt attttttatg
    25321 ttataaagaa tttatatgac taaaagacag tttaatattt tattctggga tttcatcaat
    25381 agctcttaaa agctttgcag gaacaccacc tacaatagta ttttttgtta caactgctcc
    25441 agcagcgata attgcattat ctccaatagt aactcctgga actcttcata caaaaaagag
    25501 gcttatctct attttgagat agccccactt aaccgataaa attaaataca acctaacaat
    25561 atccttactt atttattcca agttctctta gacaagcaat agtaatttca tcttgtggat
    25621 ttaattcata agctctttct atatagtaag ttgcatcatc aatatcacca ttatttaaat
    25681 aagccattcc taaattgcaa agtatttctg ggtcttcctt tatcttagcc gcttgttcaa
    25741 aatactctat tgctttatct aaattattct tataagcctc acaaagcccc aattccacta
    25801 tagtatccac ctgattaggc tttattatca atatcttctc aagatacatc ttagcctgct
    25861 ctatttcgcc cataccctta tatccaagtg ctatcataaa cagcaagttc caccaatcca
    25921 tatgttcttc ttctaatgga agtaacttct ctaatccttc cttaaactta ccttggaata
    25981 caagtgtata tccttcttca tattgaactt taaagtccat cttgcctaga ttctcttgta
    26041 cctcagctac taaatcagca tctaacccta gccttaatgc ttcttcccaa gtcagcttag
    26101 tctttaaata ttgaccttga ttataataat ggtagcctaa gtgataataa cctaatgcaa
    26161 aattctcatc aacatcaatt accttttcaa gcttagccat ggcttcaagt aagaatgcat
    26221 tcattgcctt atcatccata tctttttgat actgtttagc tatctcttgg caaacaatcg
    26281 catagttata cattgcatct aaatcatctg gatacattgt tataaatgct tttatatata
    26341 ttaaagaatc tgtatgttca ccaatctcaa agtattttct cgacatatat cccatatacg
    26401 atttcaaatc caaatcaata tcttttgata aagcatttaa aaacttctca tactctgcat
    26461 tgtttttaaa cttactatct attccaatta tatatatcat tgcatctgct atagacattg
    26521 aatttaactt atcttgagca gtcttttctt ttatcccttt tactaatacc tcatttttta
    26581 taggtacatc taatccgcct tttggcactt tatagctttt taatttaaac tctccaccat
    26641 gttttatagt tataaaagct aattcttctg ctttttttag caaatatttt tctatcttaa
    26701 aatccatttt atcctcctaa atacttaaat ctctacttta tgagttttag atttctcaaa
    26761 tattttttta tcatcatact caaataagac tacattttct acttctctta tttctgtatt
    26821 tatatcattt tttaaatcca caacattaaa tctaaatggc tcaaaatgaa catattttat
    26881 tatatgttta gctggtttat caacaaaact aggtaaatat gttgataagt tatcattatc
    26941 ttgtaaaaat acatggcatc tattcttaaa atcatccaca tctagcttat taaaaaactg
    27001 tggtatattt gatgttttac ccagtgatat atagtcatat tttaatatat cattgaatac
    27061 atcattatct atgttgattt tttcattata gaaatccatc aatattttat ataactgatt
    27121 ctttccttgt gccaaatcaa agtagccatt ttcatcaaaa tacttggcaa aaacctcaaa
    27181 gaatttaaaa ggactttgtt tataaaatct gcctataata tatctcatag ataacacaaa
    27241 gtttttagaa ttgtagtatc tctccagtat atcttctata tcctttagct tcaaagtttc
    27301 ttcatagctt atataattat tgtataaaaa ctcatatgga gcataatcct tatatttaaa
    27361 accatgctca tctgcaactt ttctcatacc tgtcccttta atcatcttta aaaagcctaa
    27421 ttgcagatgc tctataccta gattaaatac atcattaaaa gaattttcaa agctcttata
    27481 atcttcataa ggcagtcctg caattaagtc taagtgctga tgtatatttc tataagatgc
    27541 tacagtttgt acaacatgag acaacttttt aaaatcatcc cttctaccta ctgcatcaag
    27601 tgttttttca ttcgttgact gaacccctat ttcaaattga aaaagacctt ctttacaatc
    27661 agccaaaaac tcaagcatct tatcatctat caaatacgct gtaacttcaa aatgataagt
    27721 tgtatatcca ttatcatttt tcataaggaa atccatgatt tccatagcca cttttctatt
    27781 tgcattgaat gtcctatcta taaactttat ctgtgatact cttgcatcta ttaaagcttt
    27841 taaatcactc tttatcctgt ctaaactaaa ataccttaga cctggaatag tagatgataa
    27901 acagtactgg cagttaaaag gacaacctct tgatgattca taatacacta ttctattttc
    27961 atattctttt ggattcaagt tttcataagg actaggaatc tcatccaaat cttgaagtaa
    28021 ttccataggc ttattcacta ttatctcatt gccttgtcta tatacaagtc catttacatc
    28081 atttattttc atctttcctt gcaagtgaag tactaagtct ctaaaaatta attctccctc
    28141 tccataaagt atataatcca caaaatcata ctttttcatt gcctcataac tatcataact
    28201 cacttccggt ccgcccaaag ctattttagt attaggtcta atttttttta gattatcaca
    28261 aagtttaact atatctccca cattccagat atatgttgag aataatatta tatcatattc
    28321 atttttatat atatccttta aaatatagtc caaatcgttg tttatcgtgt attctttcat
    28381 attaacttct atcaaatctc ttacaaattc ttttagatat cttatagcca aatttgtatg
    28441 aataaacttg gaatttaatg ttgttaataa aattttcaaa tatttctcac ctctttttaa
    28501 gattatactg tctttttgta ccccttatag gttaaaatag tatatgtaaa gtctaaataa
    28561 tatctatata attaatttat taatttcaag taatgtgagg aaaggaagtt ttttatggaa
    28621 ttaattgaaa aagtatctat caactcttta tcaaaaagag atttactact tattataaag
    28681 tctcttgaat acactaatga aaatactcaa attagtgatt tcattgacct taggaataat
    28741 atagtaaagg aattatgttt tttaactgac actaaggaaa aagatttttt agattatcta
    28801 gaaaaaaata gcacactcta ataacctaaa cctttttaaa ctttaatata taaatacctg
    28861 atttagaatt tatttttatg cttaatatat tatcattaaa ggttttcgac ataactgtta
    28921 tttgattaaa tatagggtct attatagtat taccttcttc aagtcttata agacttcttg
    28981 ggtctatgct tttattatat gccttaactc caattttata ttttctcaat ttaacaacat
    29041 attcggacat agaaaaaata aaatctatat tatcttcatt taatttacta tcttcaccca
    29101 aaatggataa tatagatttt tttatgctat taattccttc attttctaca gttctatctt
    29161 ccatatatac atcatataga acatttaaaa catttaataa aatactttct tcatcataat
    29221 cttcagattt caaatcaatg tacttatcaa ttaattgtat tgctttaatc cttgccatct
    29281 gaaactttac aacatctgat ttgtctaatt ctatagaaaa tcctattatc ttgtctttaa
    29341 tactctgtag taattcgaca tactctattt tactattatt tataagattg tgcataatgg
    29401 aattatacac tacagaacct aataaataat caaataaaag ttcttctttt ttaaaataaa
    29461 taacatactt ttttattagt ttaataacct catcttcgct tttttgaata ttagtatttg
    29521 ataaaattag tggtagtaaa atatttaaaa aattgatatt tcctattttt tctccataaa
    29581 atctatagta aataagttca tctctcagtc tattttcgtc tatatacaca catctttgcg
    29641 ttttatcatc tattaaggat tttgtattta ttatagttaa atctttaaga gccctacttg
    29701 cactactaaa agaagatgtg tagacttttt ttagtaattc gtcattcata aatatatcct
    29761 ttctgaaaaa ttgaatgtaa ataaattgtt attaaaaaaa aagagacagc ccgtttaaga
    29821 agctgtcata tataggggga tagtagtgta caaaaatcat tgtaatctac tatctatata
    29881 ttattatacg atactacttt tttatatgta aagcatcagt tttcgacatc cctatatttt
    29941 acagttatac aaattgcgta ctaagttcaa ttttattctt tttatttcaa cattttatca
    30001 tgctacattc tttccggagc gtgcattccc aacaatttca gagaattttc taaagtttgt
    30061 tttgttgcta aaactaatgc aagtcttgct tttcttattt ccaaattttc tactaaaatt
    30121 ggattgtcat gataaaattt attaaacgct tgagctaaat ctaacataaa tctagtcact
    30181 atatgtggct catttttctt tagagcaagt aaaatattct tattgaatga ttctataact
    30241 ctaagaactt ctgcactatc ttcatcagct aatagactat aatctatatc agaagtcact
    30301 tctacttctg cttttcttaa aactgcacag catctagcat gtgtatactg aacatatggt
    30361 ccagtctcac catcaaaact aagcgttctt tcccaagaga aggtatagtc tttaattcta
    30421 ctgttagata actcttggaa tactactgcc ccaacaccta cttgcttaga tatttcatcc
    30481 acatttttag catttggatt tttagctagt ataatttcct tagttttatc tatagcttga
    30541 tttaaagcat cttctaaaaa tactactctt ccttttctag tcgacatagt accttcttca
    30601 agtgcaacca taccaaatcc aacatgaatt aaatcttttg cccaatcata tcccataagc
    30661 tcaattacct taaaccattg ttggaaatgt aagttttgtt gagaaccaac tacatatata
    30721 cacttgtcaa aatcataagt ctcttttcta tatattgcag cagctaaatc tcttgtcata
    30781 tataacgttg aaccatcatt cttagtaata agtgctggtg gcattttata ctcttctaaa
    30841 tcaacaattc ttgctccttt agactctttt aaaagatttt tctcttctaa tagttcgata
    30901 actctgttca ttttatcaga ataaaaactc tctcctgcta aagaatcaaa ttctatatct
    30961 aaaagtttat acactctgtt aaactctttt aaactctcat ttctaaacca ttgccataat
    31021 tcttgtgctt cttcgtcacc attttctaat ttattaaacc atgctcttgc ttcatcttcc
    31081 atttctgggt gttgttcagc ttcatcatgg aatcttacgt ataattttag taattctgga
    31141 attggattag actctactac ttctttttct ccccactttt taaaggccac tattaactta
    31201 ccaaactgag ttccatagtc acctaaatga tttattctta tagtcttgta tccttgcgaa
    31261 tcatatatct tatataaagc attacctata actgtagttc ttatgtgccc tatatggaaa
    31321 ggttttgcta tatttggaga agaatattca actattacag ttttatcttt tccaaaatca
    31381 ctatgtccgt aattttcttt ttcatctaaa acttttttaa ttacagtttc tgctaattga
    31441 gatttattta caaagaaatt tacatatccg cctaattgta ttacttttgt tatctctcca
    31501 cttggttcta tactttccgc caattcactt gctatcatgt ttggagcttt tctaaacact
    31561 ttggctaatt taaagcatgg aaaagcatag tcccccatat ccttatttgg tggaacttct
    31621 ataagtgcct ctatctcttc tttcgataaa tcttctattt tttctttaag gcaatttgat
    31681 attgccacct taaaatcttg cattctgttg ctcctctcta cattgttcct ggtttatatt
    31741 gtgcttatct ctttatgtta aatcaaattt cgtgtttttt caagtatttc attacagtgt
    31801 aaaacacttt aataattatg ttttctgtat atattaaaaa tactataatt gcagttttta
    31861 atcacactat tattgatttt ttataagact tttttctatt ttattgtttc ttgtactatc
    31921 cagttgactt tatatacttt ttttaatctt ttatcaattt tcatacatca atattaattt
    31981 tatatacatt tattttgctc ttttatctat ttttatacat caatattagc tttatatact
    32041 ttttttactc ttttatctat ttttatacat caatattagc tttatatact ttttttactc
    32101 ttttatctat tttctataaa tctttttcac tcttaatttt tattccttct tcttctaaca
    32161 gccttgctgt tatacctttc cctggtatct ttttacctga aaaactacca tcatatatta
    32221 gattacatcc acaagagggg ctaccctctt ttagtattgc ttcgctacat acatataaat
    32281 ttgctatttt tagagtttca ttagcccctc ttacaaagtt ttctgttaca tccttattat
    32341 ctttagtaat tacttttcca tcaactatct cagcaggtgg tcttggtgta gatagaccac
    32401 ctaactgctc tgggcataca agtgtaaatt ctttgccttt taaatattcc ttgacttttt
    32461 cattttcatt attatcgcca ctatatttac aatttattcc tattaaacat gcagatacca
    32521 atatcataaa aatgtgctcc tatacaaatt cttaatatat tcttatacaa attactaatg
    32581 tattcttata caaattattt taatttaaca attgttactc ctgctccacc ttctccatat
    32641 tgaccacctc gttgtgattt tacatgtcta tttcttctta aaatgtcttg tagccctgct
    32701 tttaatactc ctgtgcctat tccatgtatt actgttacag actctaaacc tgccacatat
    32761 gcatcatcaa gatacttttc tacttccatt atagcttctt ctaggtttaa ccctctaagg
    32821 tcaacttcat tttttacact accagatttt gaccttataa tgtttctcgt tgatttagtt
    32881 acatttgttg aaacatcttt gcgtgttttt tgtaaagatt taaaaggtag agtcatcttc
    32941 attataccaa tttgcactac tgcttctttt ctctttttat ctactgaaac tacactacca
    33001 ttttgattta aagttattac cttaacttct tcacctggct ttaagtcttt tatctcctta
    33061 tttgatacct ttggaactat catacttttt actgttggct gaagacttcc catagagctg
    33121 gtcaattctt ttctaagttc ttctatctta cgattctttt cttttgaagc tctttcttgt
    33181 tcaagatttc taagttcttt tattataata tctacttctt ctttagcttg tcttataatt
    33241 gaaaatgctt ctgatttagc tttttcaatc atcttatctc tttgactgac caatttttca
    33301 agtttttcgt catactctac tttcagtttt tcaatctctt cttttaatct ttcagcttct
    33361 tctctatctt ctactgcttt gattctattc ttttcaacat tctgtaacac atcttctaaa
    33421 gctatgtttt ctgtatttat atattcttta gcacgtgaaa tcacataatc acttagccca
    33481 agttttctag aaatctcaaa cgcatttgat tttccgggaa cacctataag taacttatat
    33541 gttgggctta aagtttctat atcaaattca actgctgcat tttctacacc tggtttagta
    33601 agagcataat ttttaagctc actatagtga gtggtagcta tacatttagc tcctacagaa
    33661 tttatatctt ctaatactgc tattgcaagg gcagctcctt caacagggtc tgttcctgct
    33721 cctaactcgt caaatataac taaagagtct gctgttacgt tttgtaatat agatactata
    33781 ttcgtcatat gagatgaaaa tgttgataga ctctgttcaa tactctgttc atctcctata
    33841 tctgcaaata cattatcata tacacacata ctacttccat aatcagcagg tatatgaagt
    33901 ccactttgtg tcataagtgc aaatagacct acagttttta ttgtaacagt cttaccacct
    33961 gtgtttggac ctgttatcac caatgtatgg aagtctcttc ctaaataaat tgtatttgct
    34021 actaccttct ttttatccag cagtggatgt cttccatttt ttatgtttaa atatttatcc
    34081 tcatttaaag ttggttctat tcctctcatc tgtatagaaa gttttccctt tgaaaatgca
    34141 aagtccaatc ttccaagtat ctcttgatta gagattaaat cttcgctcac ttctccaacc
    34201 atagctgaaa tctctgataa aattctttct atttcctctt gctctcctag tttcaactgc
    34261 ctcaattcat tgttcatctc tactatagtc attggctcta taaaaagtgt cgcaccagaa
    34321 gatgactggt catgcactat accagctact tgcgacctat attcagattt tacaggtact
    34381 acaaatctat cacctcttag agaaattatt gcatcttgta agtacttttg ataagtagtt
    34441 gaacttatta ttgaatttaa ttttgaccta atggattgat ttttttgggc tattcttctt
    34501 ctgatatctc taagtgtact tgaagcattg tcagatattt ctacttcact aattatagca
    34561 ttgtatatct ggtcttctat gtccttgtac acatatagag aatttgatag agattgtatt
    34621 attggataat taaaatcttc ttcatcacta ctagataagc tatttcttaa tcttctagcc
    34681 actcttaagg tatctgctat cataatcaga cttcctgggt caagagaagc acctatataa
    34741 gctctcttta ccttatcttc tatatcatga ataccttcca accctactga acctctcttt
    34801 ataataattg attgagcttc actagtttct tctagcatag atttcacttc tacaaaatca
    34861 gtatttggta ctaaattttc tatatatttt agacctaaag aagatgaggc tttcttcttt
    34921 aacaaatcta ttattttatt gtattctaga actcttaacg atttctcatt cattttctat
    34981 attcaccatc ctcgatattt taactttttt atttaatttt aaaaatgtat gttttcaagc
    35041 atttccaata aaaaatgcaa gttttatttc tttttcattt ctaaaattat tatatttttt
    35101 ctcttttaga tatattttac acatattttg tattatacta tgtatgtagc tatctatata
    35161 ttatacataa acatgcttta tttgtaatat aatctgttaa aaaatcttct ttaatatttt
    35221 taaaggagtt tattttagct tataaaaaaa tagctactta tatagataaa ttaaggagga
    35281 acatgagtca tgggaaacgt attagaagga ttaaaaccag aaagtgtttt taaaaacttt
    35341 gagaaaatat ctcaaattcc tagaggttct ggaaatgaaa aaggcataag tgatttttta
    35401 cttagctttg gtaaaaattt aggtcttgaa actattcagg atgaaagctt aaacataata
    35461 ataagaaagc ctgctacaaa aggatatgaa aattgtccag gtgtagttct tcaaggacat
    35521 atggatatgg tttgcgaaaa agaaaagaat gttgaacatg attttcttaa agaccctata
    35581 aaattaagaa ttgatggtga catgatatat gctactggaa ctactctagg agctgacaat
    35641 ggtatagctg ttgctatggg tatggctata ttagaagata atactttaga gcatcctgca
    35701 ctagaagttt tagttactgt taatgaagaa gatggtatga atggtgcaga tgcactagac
    35761 cctagcttaa ttaaaggtca atatatccta aatatggact ccgaggagga aggatactta
    35821 ttagttagct gtgctggtgg taaaacttgt gttgttagtt tacctgttga atacaaggaa
    35881 gttaaaggtg acaaacaagg attattagtt gaggttactg gactattagg tggacactct
    35941 ggtatggaaa ttgttttaca aagagcaaat gctaacaaag caatagctag agtattaagt
    36001 gtattgaatg ttgattatga attagcttct gttgatggtg gtactaagca caatgctata
    36061 cctcgtgaag ctaaatgtgt aatagctgtt aataaagctg atgttgaatc tgctaaaaaa
    36121 caaataaatg acattttaac tgcatttaaa catgagttta ctacttctga ccctggcatg
    36181 acttactcag tagctgaaac ttctgtagac aaagttctta ctaaagattg taaagaaaaa
    36241 gttgttcaaa tgtcttgctt aactcctcat ggagttcaat cagtaagtct tgatatagaa
    36301 ggattagttg aaagttctac taacttcgct ataattgaaa ctaaagaatc tactattgaa
    36361 tttttaactt ctgttagaag ttctgttatg tctattagag atgaaatcgc tgatagaata
    36421 agacttttgg ctcaagcttt aggtgctaat tacgacctta tagctcaata cccagcttgg
    36481 gaatttaaaa aaggctcaaa attagaaaaa atatgcagtg aaacttatga gaaattaact
    36541 ggtaaagttc ctactgttat ggctctacat gctggtcttg agtgtggatt attacttgat
    36601 aaacttcctc atgctgaagc tatctctata ggaccagata tgtttgatgt tcatactcca
    36661 aatgaacatg ttagtatccc ttctgtagct aatgtttggg attatgttat tgaaatatta
    36721 aaatctatga accaatatta aatttaagtt ttgattctaa atagatttta attttaagta
    36781 atctatataa aattaactct gtaaattaat actactgtta gtataatatt tactttcaat
    36841 attaacttat agaaataaag cctaatgata gataataaca tgtatcatta ggctttattg
    36901 taatttaaat attattaaac tttattctgt tttaaaatta attcttatac tattttaaat
    36961 ctatttcttc cttgtctctt agaatcataa agagcattat ctgctatttg atacagttca
    37021 tcatactctt gaccacgctt tgttataact acccccatac taatagataa actttctcca
    37081 tttggcaact taatgagatg aatatgattg tatacatcat tacatttatt gataagttct
    37141 tccttattta ttatatcatt tatataaaca ctaaattcat ctccacctaa acgacctact
    37201 atatcatctt ttgcaaaact ttcatcaagt acctttgcaa catcaatcag tactttatct
    37261 cctaccaggt gaccataagt atcattaatt gacttaaaat aatcaatatc tataatcaat
    37321 aaagctccat ttttttcagt tgataaattt aataaatttt gtgtagttaa ttttctaata
    37381 tatgctgcat tataaatatt tgttaaacta tccctttgaa ctttatccaa caatctattc
    37441 ttttcctctt tttcagaatc aatattagct attttaccaa ttacatacat tggtattttt
    37501 ctatcattat aaattatttt tgaagtaatt ctaatccaat gaaaagtatc atcaagcatg
    37561 atagaataca tttcagtagt cccattttct tgtgatttta ttacatcaaa aaacttttta
    37621 ttatattctc ttcttatttt atctctttca ttttctatat tgccattttc actttcctta
    37681 tcacttttat ccaaaccata ataagtctca ataccctttt ctggcataga aattatcaat
    37741 ttatcttgct tataatcata ctcaaaaaag tattcacttg tcatttcata caactgagta
    37801 tgttttttga gttccaaaga tatcttttcg ttcatacttg tacgagtatg taaaccaaat
    37861 gccataaatc caataataca aattaataaa cttgctacaa taaaaatagt ctcaattgga
    37921 ttctgttgca taatataaag caacgagaaa tcttgtctat atgtagtatt ttggtagaca
    37981 attgattgca ttttttctga tgaaatactt ataatagctt tattaaatat attcaataat
    38041 tcttgtttat cttttttaat tatgccaaaa caaatactct ttgcttcata agtttgagga
    38101 actaggcgca catttttata cttaggttga ttcatatagt actgcataga ataaccttct
    38161 ccataagtgt aatctgcata gcctttatta acagcttcta tacactcttc taaattatca
    38221 taccaaacta tattttcttc aaactgacca taataatcaa tacctttggc tagagctata
    38281 cgctttccat ttagactatt ctcatttaat ttattattaa tcatcatagt atattgagta
    38341 gaaagaaaag gccgagacat agctatatta tgttcacgag agtactcata atcataaatc
    38401 attcctgcta caatatcaat ttcatgtttt ttagccatat cttccaaatc ttcttgagat
    38461 tttacaggca caagttcaaa ttccaaacct gttttatcac atatatgatt taacaaatca
    38521 attgatatac ctcttaattc tcctgtatct ttatctgcat tttgaaacgg tggtttgttt
    38581 aaaagtactc ctacttttaa tttttctgta tctttaatgt aattagtttc ttcatcagat
    38641 aaaaacattt gattattctc tggcgtaaag tatttctcat ataaagtagt tgaaaaatat
    38701 gggtccttct gttcaatatt attaattgct gaattaagct tttgagttaa gtctgtactg
    38761 ccttttggag taatgaaata aaatggcttt gatgagaatt ttgctacagt acgcacacct
    38821 tctattgggt tcatactaac attaagaagt acatctgctt taccactctt tagtacttct
    38881 aattgctcgt cacttgtttt acaatatacc aattcaggtg acataagatt cattttacaa
    38941 tactcatcta attctttaag cctagtatca gaattttcta ttacagcaat acgaattttt
    39001 ttcacatctt gcgaactcat tatgttatca atatctgtat tttcatataa tgcttgtagt
    39061 actgtatttg ctactccata actgtttccc gcataattat atattttttg cattggctca
    39121 ttgtataaca tacctcccat tatatcaatt tcaccatttt ctaacatctt cattaatttt
    39181 gtaatagatt catttatctc tccaggaact tgaacaaatt cataattcca tccagtatac
    39241 tgtgcaattt ctagcaaata ttcatatgta tacccaaaat agttaccttg ctcatctatt
    39301 tcagttaaac ctttttgaat aggataagca acacgaatgg tacgattatc attacctata
    39361 gcattgatac caaaagaaaa tatgcaagaa gtgaatatag atgtaattac aataattaat
    39421 aaaagtaatt tttttaaatt cactacacaa tcctccatat agttaagatt tacagtataa
    39481 attactgcat aaacttgtag acatgaaaaa atagattagt atataaaagg atatataaat
    39541 ctacttagaa atttaaatat ccttaatata tacaatatat ttaaattata actcatttat
    39601 atatctatta caacaaattt accctgctaa atacctactc tatatcccat aattttacaa
    39661 gttgtaatga agtttaagtt ctatctcatt tcaaatttag cagtgtttct atttacagtt
    39721 cttttatatt tcacatctgg ataacccaat ataaatgaag tgataactgc ttcgttctcg
    39781 ctcattccta acagttcttt tatctttggc tctacatttg atgccataac aaaaaatcca
    39841 ttataacaaa ttcctaatcc ttgagaatag gccataagtt ccatattaga tgcagccaat
    39901 cctccatcta cataagcact tccacctaga gacatatcac ctacaactat cataagtagt
    39961 ggggcatcat aaaataaaga atcatttccg ttctctttat atcttttgta cattttctta
    40021 aatgtatttt tatagataga acgtacaggg tcattgtcat cagtatctaa tgccaaatta
    40081 taaagacctt gtattgccag ttctttcact tcttttagct tttcttttac taatatgtat
    40141 cttataggct gtctattacc acctgttggt gtgtatctac cagcttccac tatgtttttt
    40201 attttttctt cttctacctc tatatcttta tattgtctta tacttcttct aaatttaata
    40261 aaattcaata acttttcggg ttctaattca aaactttcac gattgtattc tattacttca
    40321 tttttatcaa atccttgcat ttctattgca tctgttgggc aaattgccat acagtgacca
    40381 cagttaatac agagaatatt tttaaccttt gctttatcat ccttaacttt tattgcattt
    40441 acaagacagt ctgattcaca catcccacag cctacacatt tttctaaatt tatgtctatc
    40501 attccaaatt attcctcctt ttaaaaaatt tactttagta taaaacaaat agttgtctaa
    40561 gtcaattttt atttttttaa caatatacta gccttaacta cactttaatg gagaaaaata
    40621 tacataaaat tagaacttaa attagaattt attcaaaata aaagataaaa gaagtaaatg
    40681 tctaaaagtg aactttttag ttaatgagac attctttatt gtatgaaatc aagtggatat
    40741 ttatcatttc aacaataaaa aaggcttacc tctattttgg atatgtcctt gttaagtaga
    40801 cagactaaaa aaggctatct ttatgcactg agatagcccc ttttcccaac tactattgtt
    40861 tattttaata ttatatcaat aaatttaatt tgctataacc tgtaagttac attttaattt
    40921 gatgtaactt taaattacat ttttaagtaa tatctttaaa ttatattctt aaaatatatc
    40981 attaaactac attttaatta atctaaaaac tatatcttaa ttaatctaag aaacttacat
    41041 tataatcttt tagtttccca ttatcatata caatctttgc actaaagata tttccagtct
    41101 tagcccaatt taagaacaac ctgtcacctt cccacatatt taaatcgaat attttatcat
    41161 taccaaccca aattaaattt ccttctttac aatcatttaa atctttttca gacaattcac
    41221 cttcaaactc atctgagaaa tacaaaaaca tataccaatc ttcttcatct ttaaattttg
    41281 gaaaagttat caacccttta tatgacaatg atttaagagt aagaccagtc tcttccttta
    41341 cttccctaat tacacattct tctggagtct ctccttgctc aaactttcct ccaactccta
    41401 catacttacc ttcatgtata tcatctttct ttttatttct gtacaacatc aaagtcttgt
    41461 catctttttc tatataacaa atagttgtaa gtttcatgta tctcgcctta ctttctcttt
    41521 atattctcaa ttaattattt tcagcacttt caagcacatc acttatattc ttcattgcat
    41581 taagagaaag ttctgcaaat tcatcaaatg gaatccctat atcttctata cttctcattg
    41641 catctctatt tacatttgaa gcaaatctcg tttctttcat tcttttaata acagacttag
    41701 tctttacact agtaagtttt ttatctggat aaactaaagc tatagcagtt ataaacccag
    41761 ttatagggtc tgctgcatat attgcttttt ccattaagct agttcttgta actcccattc
    41821 catcatgatg accaagtatt gctctataca tttcttcgtc accgaagttt tcttttttaa
    41881 gcagttctac tgttgttgta gcatgtcctt cacatatttc tcctggcttt cttccaacta
    41941 tatctgaatc caaatcatgt aaaagacctg ccactgccca ctcttcttct ttttctggct
    42001 ctagtctttt ggctaattct ctcataacag cttcaacagc ataacaatgt tttctcattc
    42061 tatcagtttc taaatactta aataaaattt ctaaagcttc ttctctcatt ttcaccaatc
    42121 cccctgtttt ttcacatata tgatattttt atatattaat acacataaat tcaagtcttt
    42181 atatatattc atgtatattt ttcatgttat atctattcta cacctttata gtagtgtcct
    42241 gcaactgttc caatttcatc taatttctta tacaaatctg tagccttttt tccaagttta
    42301 taattattac tcaatacctc aatgtgagct tcaataacct ttttaaccat ctcactatct
    42361 tcaactgtaa aatctatcct aaatacatct attccaattt cctctaactc atatagattg
    42421 tccaacatac aagtaacctt cgaattgtaa attgtacttc tacagaatat atcttgtgtt
    42481 atcctataac ttgcatccgt aaagtctctt aaagcataac tactttcctt acatttagca
    42541 actctcttgt cctttttaca atctctaaca actactccca taggacagta ttctgtaacc
    42601 atcataggag catatccata tattactgct tcaatttcta catctgtata ttttaaagtt
    42661 tgtttaattt catttaagtt tagttcttgt gacaaacaaa ctgtacttgc tccctcttct
    42721 ttgaagtggt ttatactttc actattgaat gcatttaagt agcaatctat atttaagtct
    42781 tttttcttaa agtattccat agagccccag ttaccaattt gaaccttatt aattcctttt
    42841 atattatcca atatctcaaa ttgcttgtac tctttatttc taacaatcct tggtgctgaa
    42901 tagattaatt ttttgtcagt gactgctgta ttatagtggt tcttattcaa agtatctatt
    42961 ttacttgtat ttgacaaagt atctatgtta tttgtatttg aacccattag ttctttagga
    43021 atacctaaag acgaagaagt atctaaatcc atagaaatac ttg
//
LOCUS       DAEPLF010000011        42855 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEPLF010000011 DAEPLF010000000
VERSION     DAEPLF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42855
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.011"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(74..622)
                     /locus_tag="KR314_000549"
     CDS             complement(74..622)
                     /locus_tag="KR314_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326953.1"
                     /translation="MEGLGFYAAALSGSSYQRIGFGKLDPIEVIADGDWISYKQAQDT
                     LTVIRNFLNSFDWRNASEMERANRAAKLVTEAKYVDSKYCNIVYGNLVDKRGVCGSFA
                     SSFHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRSDGSEISAFGGALDFDYRKLK
                     DATREMTTYYDAKALSILGFNQ"
     gene            complement(724..1422)
                     /locus_tag="KR314_000550"
     CDS             complement(724..1422)
                     /locus_tag="KR314_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326954.1"
                     /translation="MKISSQYRSQYSFRHESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            complement(1958..3025)
                     /gene="rsgA"
                     /locus_tag="KR314_000551"
     CDS             complement(1958..3025)
                     /gene="rsgA"
                     /locus_tag="KR314_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBH0326955.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKSGCTVALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
     gene            complement(3022..3423)
                     /locus_tag="KR314_000552"
     CDS             complement(3022..3423)
                     /locus_tag="KR314_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBH0326956.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      4219..4315
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4369..4545
                     /locus_tag="KR314_000553"
     CDS             4369..4545
                     /locus_tag="KR314_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326957.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            4955..5593
                     /locus_tag="KR314_000554"
     CDS             4955..5593
                     /locus_tag="KR314_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0326958.1"
                     /translation="MSNQIKDFDITNDMFQESIPITTKTILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLVKAANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(6071..7429)
                     /gene="rlmD"
                     /locus_tag="KR314_000555"
     CDS             complement(6071..7429)
                     /gene="rlmD"
                     /locus_tag="KR314_000555"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBH0326959.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAEGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(7604..9364)
                     /gene="pyk"
                     /locus_tag="KR314_000556"
     CDS             complement(7604..9364)
                     /gene="pyk"
                     /locus_tag="KR314_000556"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBH0326960.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEILTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(9416..10375)
                     /gene="pfkA"
                     /locus_tag="KR314_000557"
     CDS             complement(9416..10375)
                     /gene="pfkA"
                     /locus_tag="KR314_000557"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBH0326961.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(10584..14153)
                     /locus_tag="KR314_000558"
     CDS             complement(10584..14153)
                     /locus_tag="KR314_000558"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBH0326962.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(14310..15257)
                     /gene="whiA"
                     /locus_tag="KR314_000559"
     CDS             complement(14310..15257)
                     /gene="whiA"
                     /locus_tag="KR314_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBH0326963.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(15274..15699)
                     /locus_tag="KR314_000560"
     CDS             complement(15274..15699)
                     /locus_tag="KR314_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH0326964.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(15768..16874)
                     /locus_tag="KR314_000561"
     CDS             complement(15768..16874)
                     /locus_tag="KR314_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBH0326965.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(16886..17743)
                     /gene="rapZ"
                     /locus_tag="KR314_000562"
     CDS             complement(16886..17743)
                     /gene="rapZ"
                     /locus_tag="KR314_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBH0326966.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIDEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(17774..18487)
                     /locus_tag="KR314_000563"
     CDS             complement(17774..18487)
                     /locus_tag="KR314_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBH0326967.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYNDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFKLA
                     MKTISDSNLDISLVENIII"
     gene            complement(18491..19405)
                     /gene="murB"
                     /locus_tag="KR314_000564"
     CDS             complement(18491..19405)
                     /gene="murB"
                     /locus_tag="KR314_000564"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBH0326968.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(19678..20448)
                     /locus_tag="KR314_000565"
     CDS             complement(19678..20448)
                     /locus_tag="KR314_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBH0326969.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            20766..22247
                     /gene="cls"
                     /locus_tag="KR314_000566"
     CDS             20766..22247
                     /gene="cls"
                     /locus_tag="KR314_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBH0326970.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            22565..23062
                     /locus_tag="KR314_000567"
     CDS             22565..23062
                     /locus_tag="KR314_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBH0326971.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLTTKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            23079..24965
                     /locus_tag="KR314_000568"
     CDS             23079..24965
                     /locus_tag="KR314_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBH0326972.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            24988..26769
                     /locus_tag="KR314_000569"
     CDS             24988..26769
                     /locus_tag="KR314_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBH0326973.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRICMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(26909..28705)
                     /locus_tag="KR314_000570"
     CDS             complement(26909..28705)
                     /locus_tag="KR314_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBH0326974.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQILSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLSPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(28919..29848)
                     /locus_tag="KR314_000571"
     CDS             complement(28919..29848)
                     /locus_tag="KR314_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBH0326975.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            complement(29848..31665)
                     /gene="uvrC"
                     /locus_tag="KR314_000572"
     CDS             complement(29848..31665)
                     /gene="uvrC"
                     /locus_tag="KR314_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBH0326976.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVEAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            complement(31924..34749)
                     /gene="uvrA"
                     /locus_tag="KR314_000573"
     CDS             complement(31924..34749)
                     /gene="uvrA"
                     /locus_tag="KR314_000573"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0326977.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            complement(34768..36738)
                     /gene="uvrB"
                     /locus_tag="KR314_000574"
     CDS             complement(34768..36738)
                     /gene="uvrB"
                     /locus_tag="KR314_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBH0326978.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            complement(36925..38187)
                     /locus_tag="KR314_000575"
     CDS             complement(36925..38187)
                     /locus_tag="KR314_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBH0326979.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKVKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFDLKLDGVEKEAFDFIM"
     gene            complement(38250..39554)
                     /locus_tag="KR314_000576"
     CDS             complement(38250..39554)
                     /locus_tag="KR314_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0326980.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INRDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            complement(39585..40445)
                     /locus_tag="KR314_000577"
     CDS             complement(39585..40445)
                     /locus_tag="KR314_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBH0326981.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSMEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLILQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYIPLIIPFMTS
                     SFGIFLLRQNFMQISDETIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            complement(40489..41358)
                     /locus_tag="KR314_000578"
     CDS             complement(40489..41358)
                     /locus_tag="KR314_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBH0326982.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            complement(41342..42412)
                     /locus_tag="KR314_000579"
     CDS             complement(41342..42412)
                     /locus_tag="KR314_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0326983.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGKLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
BASE COUNT        13470 a         7508 c         5242 g        16635 t
ORIGIN      
        1 aaattttcat ataaaattcc ttattaaatt tcaaatcaat aaaaaaaggc tctttataaa
       61 gcctccccta tttttattga ttaaatccta atatagacaa tgccttagca tcatagtaag
      121 tagtcatctc tcttgttgca tcctttaatt tacggtaatc aaagtctaat gctccaccaa
      181 aagctgatat ttcagaccca tcagacctat accatttccc atcaatttgt atatagttcc
      241 aagcatgatt taatgatgga tttaatatag atagtgaatc cattcccatt aaccttgtta
      301 ataaatgaaa gctacttgca aaacttccac atacaccacg tttatcaacc agattaccat
      361 agactatgtt acaatattta gagtctacat attttgcttc ggttaccaat tttgcagctc
      421 tattagctct ttccatctca gatgcatttc tccagtcaaa actatttaaa aaattcctga
      481 ttactgtaag tgtatcttga gcttgtttgt aagaaatcca atctccatcg gctattactt
      541 ctatagggtc aagttttcca aagcctattc tctgatatga gctacctgac aatgctgctg
      601 cataaaatcc tagcccttcc atacccttat ttatataata atatttacca tctgctcgaa
      661 gctcaactcc atttggattt cctccataga gtaaattaaa cttatttttt taaaacatat
      721 tgtttaaatc agtacattta tatgtttttt ttgttcatct gttttatcaa atatattagt
      781 ctttgaattt tgtttgtatt cttcagcatc tttatctaac ttttctattc tcgactcaag
      841 tttcattatt tcttcttgtg ttaaatttat aaaattttta agcatatttt caagctcttc
      901 acgagtttta cctactaaag ataagtcctc agatactttc tcttttttat tagaatcctc
      961 tttataatcc tttttttgtt ctaactcttc taatttttca tcatttaata tttcttcttc
     1021 aattttttca ttagactctt tagtgattaa agattctttt tcatcatttg tatttagata
     1081 agttgaattt tcatctaaca ttgtactttt atttttatct tctataatgc tatttttaat
     1141 acctatttct atattagaaa agttattagt tgtttctctt atttctgatt tatataagtt
     1201 cttaattgct tcatttattt ttttagtata aacctcttgt ctatatttta aatctctaat
     1261 tctatcatta agttcttgct tttgtttcat aatattagat aaataagtgt cttttcctac
     1321 agtttcattt ttcttaacca tactttcatt tattcttgta ttatttatat tactttcatg
     1381 tctaaatgaa tattgacttc tatattgact agatattttc atttatttca cctctctaaa
     1441 ataaatttat gtttataaaa accaacttaa cttaattata ttttatcatc aaaagtatac
     1501 atttacaaat attagttaat agttttatag gttcgagcat tgttttcaac aaaaaatttt
     1561 tattataaat taaaacaata atttttataa atatcttatt tgtattttat aattcatata
     1621 tttttacttt ataaattagc aatattgcag ttttgctctt aagaaacatc tctatcatac
     1681 ataagcatat actctattat gtattagtat aaatatgcct tttcttatct tacataagac
     1741 ctctttataa aggagactat cgtaacaaat atgtatacat tatcacatat atatatcatc
     1801 atataaaaaa tcaaacttta ttaaaaaaat taataaattg ctttaaataa taccacaaac
     1861 caaatttgcc atcaaaatca cattaaagta acaaattaat ggcaaattta atacattaat
     1921 agtatataga ctcatagaat gaacaaaaaa actacactta tcttctaatc gtcttattta
     1981 tttttgagat atttttaaat ttcttttctt tttttgctaa gtattcgctt ttattgtctg
     2041 caaatactgc ttcagtttta agcctacgat aggattccca tcttttaggc gaaagttctt
     2101 gatgagcaat agcttcccag atagcacagc ctggttctga ggtgtgagta caattacgaa
     2161 aatgacattt tccgaaataa gcttctatat ctgcaaaact tttatctaaa ccttctgaaa
     2221 catcccacat tcccaactct ctcatacctg gtgtgtcaat aacaatacca ccacctgaaa
     2281 ttaaaaatag ctctcgtcta gtcgtggcat gtcttccttt atcatcatct atgcgaattt
     2341 cctttgtatt taaaaattct cctcctagca gacaattaat taatgaagat tttccaactc
     2401 ctgaagagcc aagtaatgca acagtacagc ctgatttgat atacttagaa atctgtttat
     2461 aaccatctgg ctctatagat gaggtaacta ctacatctac acccaaggct atagaattaa
     2521 ctacttgtaa tttacgatgc acattgtcac ataagtctga tttagtaagt actactactg
     2581 ggatagctcc actttcccat gttaaagaca gatacctctc caatctatga atgttaaaat
     2641 ccttattcag tgacatgcac aaaaaaacaa tgtcaatatt agctgcaacc acctgttcct
     2701 catttacctt tccagcagcc ttacgcacaa acaagctttt tctatgaagc acatgatgaa
     2761 taatagcatt accattttta ttattgcaac gatccaacat aacgaaatca ccaactgtgg
     2821 gaaaatcaga ttgtatagta gcctcaaaac gcagttttcc tgaaacctct gcaaacagta
     2881 caccatttac acatacagct tgataaaagt tcttttcctg cgagataatt cgagcaaggt
     2941 ataaatcgtg atattccata ctttgagtaa taaaattcct atttagacca tagtcttgta
     3001 aattgatttg tttcatacca ttcattctgt ttcctccttt tccaactttt caatgattct
     3061 ttctagtggg aaagggggct gtgccagtgg ttttaatgca attgacatta atttcactga
     3121 attatcatca gctgcaatac gattagaact aagatgtcca cagcgtgtac aacgatgaat
     3181 aattgcccac tcaccatttt ttctcaccca cactccaata ggttccatat tagcatcaca
     3241 atctgcagca cggtcaccag gaatgatatc tacatgaaga ctatgtaaac aatgtggaca
     3301 atgatttcta tgttgtgtac cagctccttc aggaactaca aggcaaccac aatttttgca
     3361 aataaagtta tccatacagg aattttcctg ataataagct cgttttctat ttttataatt
     3421 catttttatt tcctccgtat cttgtatttt ctacaaaaaa agtccacagg tatttatatc
     3481 tgccttttta tgtggtatac atatttacct acacaaaaac agaaaacccc tgtggactag
     3541 aaaatttagt atcttctatt tcaacataat tatattcaaa ataatactgt gtaaaattaa
     3601 taaaagtgta gcagattaaa gcccactaca ccacaattta acaaagtaat atgtaatatt
     3661 attatttgtt gtctaatgtt agttatctat aaaggcagag taagacacaa caatcaaatg
     3721 tatcaaaaat acacttaaaa attctgatta aacagaaccc atatgcgtct ccaactattt
     3781 atttgttggt taccattata tataactctg acatcaggca agacactcct tcactaataa
     3841 ttattattaa aattttatca taaaatataa gtatgttcaa gtataattta atatattcat
     3901 atatttatgt ttgtctcatt ttcttattct ataaaatcta gtggtatttt aaaatttaat
     3961 gttcacctag tattaataat aaaaattaat tgctattatt tgttttctca ttttatgcct
     4021 attgctcaat ttatctcact taaattaata acaactgtct tttcatacaa aacctacaat
     4081 caagtttttc catatattct acatgaatag tctatttttt ttatataaaa taacgttcat
     4141 atttgtaaat ttatttaatt ttcaaataag tatattgaat aataagcaat tctattgtat
     4201 tattgtaatt gtagaataaa actaaatcgg caaaactaga gaaatttagt gacgcaaagc
     4261 tatagggact aacgcttata aaataaacta tgagttatgt cagccagttg ccaaaaagag
     4321 atattctttt tgtttttatg aaagtttttt agggggataa taatatatat gtttagagat
     4381 aaaatggata gatgtacaca tatgttgact gcttatattg gtagttcata tgattattgt
     4441 gattttatag atacacaact agatgatttt atactagagt acggagaaaa tgtggtagaa
     4501 tcttgtttgc atcaagtgat ggtattagta agtaagtata attaaataga tttggttttc
     4561 tagagaaccc tttttagttt tatctacttt aaatagcctt atgttagaaa gctaattaag
     4621 atagatttct agtattcata attttatttt ctatttaaaa tacatataat aaagatgtaa
     4681 atttgtatca aataataaat atgagaactt aaattctctt atctatgtga aaataaacat
     4741 ctcttaaaat gaaattctgt aaacataaat gattttttag tttgaaggat tagattaaca
     4801 aaattataaa tttgcaaata agctatatta aaaaatataa taatttttta tataacttat
     4861 tcttttataa cttttaattt taaaagagga gaatgtatat aagagtattc aatgtactag
     4921 aaaaaatcaa aagaaatatt agaagaaatg gatattgagt aatcaaataa aagattttga
     4981 tattacaaat gatatgtttc aagaaagcat tcctatcaca acaaaaacta ttcttgaaga
     5041 catgccttca aatgatgaat taaatcatgt tttttcaaaa ggttgtgaac gaaaaatgaa
     5101 aaagagaaaa attgtattaa tcacactact attaataggt gttttgttat taggaagtat
     5161 cctatataat ctttttttag taaaagcagc taatatttca atgctaaagg aatcttggaa
     5221 ttttgatata ccaataccta acaaagaaat tgaagtattt gatactcaag atagtattaa
     5281 tggagatgga caaagttatt ttattcaagg attttctgaa aagaatttca aaaaagtttt
     5341 taatttaaaa ggtggtatag ttgtaagcaa agataatatt aatgaaatag aaaaatatat
     5401 tgataaattt aaaagagatt ctgttaacat taataaaagt aataaaaata aaattgaaga
     5461 agattttaaa aaatataagt tagaagttaa aaaagatgat aagtatatat ataagagaaa
     5521 ttatgaaaac tatgtagttt taattataaa aaaagatgaa caaaaattat acagtttaat
     5581 atggaatcag taggtacata tgtattatta agaattataa ataataaaaa taaattttag
     5641 tcaatctaca aaaacctttt aaattgtctg gaaatcacct cctcatttgt tataaaaaca
     5701 tacaacaaat gaagaggtga ttttatggtt tttaaaaaaa taattataag tggatatgtg
     5761 ctttcacatg tttatcaaca tttgtaatgc ctatatttgc taatgaatta taatataaaa
     5821 atattaaata atattcaact atagattatg taaattatcc tatgaaaaaa ataagttgta
     5881 taaaatctgg caactgtaga ttgacgattt cttttaaaat tacaattata ataagtttta
     5941 tagaattaaa aataaaactt tagagatttt tgataacatt gtagactaaa tttgccaccg
     6001 aaattacttt acagtggcaa atcggtggca aatacaatat attattagta tataatctat
     6061 ataaacatta ctattgcttg attatcttag ctacagtttc cacatgtgta gaatgaggga
     6121 acatatctac aggttgtgcc tcaacacatt taaatccgtg aacatttaga tagtctaaat
     6181 cccttgctaa agtagaagga ttacaagaaa catagacaat tctttccgga ttcattgaaa
     6241 caatagtctc taataccttt tcgtcacatc cctttctagg tgggtctaca actactacat
     6301 tggcagtctt accctctcta tacatcttag gtacaatttc ctctgccttt ccaacataaa
     6361 attcagcatt atgtatattg tttaatttag catttatctt agcatctttt atggcatcat
     6421 ctataatttc tattccataa acctttttag ccttttgtgc caaaaataaa gatattgtac
     6481 ctatcccaca gtaaatatca aaaacagtat cagtatcatt taaatttgca tattcaagag
     6541 ccttatcata caatacttta gtttggacag ggtttacttg gaaaaatgat aatggtgaaa
     6601 tctcaaacac taagtcacct atataatcgt ttatttttcc atctccatat ataactatat
     6661 tttctcttcc tagaatagca ttcgtatctt tttgatttac atttacaacc aaagtcttaa
     6721 atccaggtat attttcctta agcactgaag ctaattcttt aagatatgga agtttgtttc
     6781 catttgctac taatacaacc attacttctt tagttgtaaa acctattttt gttactaaat
     6841 gcctaatcaa ccccgtgtga gtcttttcat catatatact taccttatag gcccttatat
     6901 atgtttttat tattttaaca attttatcat ttatctcatg ttgtattacg catttttctg
     6961 tcgatattac atcatgagtc ttctttttat agaagccaat aattggaata ccattattct
     7021 tttgtattgg aaattgtgct ttatttctat atctatatgg attatccatt cctatagtgt
     7081 catgaacttc tacatcctct aacttaccta ttctcgaaag tacttgttta acttcatttg
     7141 ttttcatttc aagttgttta ttataatcta aatcttgtat ctgacaccct ccacaatccc
     7201 taagattgtc actacacact cttttcaccc tgaatggaga cttttctatt atctcaacaa
     7261 tatctccctc agcataattt ttctttactt tattaatcct aactttaact ttatcatgta
     7321 ttaatccgcc ctctacaaaa actgtaatac cattgtactt tcctactcct actccgcctt
     7381 gaccaatgtc tactatatct acaatataca tctcgttttt cgaaagcata aatcaccctc
     7441 caatcaatct attatattta aatctttaca aacttttttc tttaaattta tattaaaata
     7501 ttgctattaa ttaaactata ttttttacta agctacaata ttttatttta ccaaaaaaaa
     7561 aggagcttat aaagcccctt agtaaataaa tttattattt ttcttataaa actctagttg
     7621 aacctctata taccacgcct cttgatgcat ccacagttac aacttctcca tcttttacta
     7681 gatttgttat atcagttgca gacacaataa ctggtatatc taaatttata cctacaatag
     7741 ctgtatgtga tgtcattcca ccattctcag ttactacagc tcctgctttt tctatataat
     7801 tgttcatttc agcatcagta gcagtagaaa caagtatgtc gccttcatta aagtctacac
     7861 aagcatttcc attctttatt atacgaactt taccttctac cgtttgagta cctacaccta
     7921 taccttgcac gatttcttcg cttattacat gaacctttat taaattagta gttccactta
     7981 ctcctactgg tactccagca gttataacta ctaattcccc attatctata taatcagctt
     8041 gtctagctgc ctcaattgcc ttttctataa cttcatctgt atttcctgca acagaagact
     8101 taactggata tactccccat gtaagagcta atttattcat agtcttttca ttatttgtag
     8161 tagctattat tggagatttt ggtctaaatt tagataccat tctagcagta tatcctgaag
     8221 aagtagatgt tattatagct gatgcattta aatctactgc tgttgtacaa gttgcatggc
     8281 ttattgcatc tgttacagta acattgttag taccattttc ctttagtaat ctatcataat
     8341 ctaaagtctc ttctgttctt tttgctattg tagccatcat ttttactgcc tctacaggat
     8401 attttccagc agcagtttct ccagataaca ttatcgcatc tgttccatca tatatagcat
     8461 tggcaacgtc tgtaacttct gctcttgttg gtcttggatt tcttatcata gaatcaagca
     8521 tttgagtggc agtaacaact ggctttgcta attcattaca tttttttatc atcatttttt
     8581 gaactattgg catctcttca gttggtatct caacacctaa gtcacctcta gccaccatta
     8641 taccatcaga aacttttaat atttcatcta ggttctctac accttcttgg ttttctattt
     8701 tagatattat ttgtatatca gttgcattat tgttctctaa tacttctctt attgctagta
     8761 catcagatgc ctttcttaca aaagaagcag ctatataatc tattccttga cttattccaa
     8821 actctatatc acttatatct ttaggagtta ttgctggtaa atttatctta acaccaggta
     8881 catttactcc tttatgattt ttaactattc ctgaattttc aacaacacaa actatatctt
     8941 ctccatttat ttcttttact cttaagccaa ctaaaccatc atctattagt atagaatctc
     9001 ctgatttaac gtcttcagca agacctttat agcttactgt acacatttct ttagtcccca
     9061 taatatcttt catagttata gtgaactttt gcccttcttc taaaaatacc tctgggtctt
     9121 caaagttacc tgttctaatt tctggacctt tagtatctaa taatatagca actggcttat
     9181 ttaatttttc tctaactttc tttgccatat caattctttc tttatgttct tcatgcgaac
     9241 cgtgagaaaa gttaaatcta cacacattta gaccattttg cataagttga gttaaaattt
     9301 cctcactttg tgatgctgga cctagtgtac aaacaatttt tgtcttcttt acattattta
     9361 acatatttgc tcctcctgag atattattcc acagatttat ttttaatact aaaacttata
     9421 tagataatat ttttgccatc tcataagctt ctttatcaaa tactttcttt tgagctaaag
     9481 cttcacttat ttctaaatca attattttgt tttcttttat tcctacaact ctagctgatt
     9541 ttccatctaa taataattct actgctctta ctccaagtct acttgctagt attctatcag
     9601 atacagttgg gcttcctcct ctttggacat gccccaatac agtgactctt aaatctgctc
     9661 ctgactcttt tttaagttcc ttttctaaat cactagcact tccgactcct tctgctaaaa
     9721 cgattatact atgtctctta cctctttttt gagtagtctt taatcttaga gcaacttcat
     9781 ctactgtaat ttctacttct ggtactatta tagtttccgc tcctcctgca agacctgcat
     9841 ataaagctaa atctccacag tgtcttccca taacttctac tatgttaact ctttcatgag
     9901 atgatgatgt atctcttatc ttacctatag catctataat agtattcatt gcagtatcaa
     9961 atcctatagt ataatctgta tatgctaaat cgttatctat agttcctggt atgcctatag
    10021 ctgggaatcc taagtcacta agtttttggg ctcctgcaaa tgaaccatct ccaccaatta
    10081 caactaagca atcaatttta tactttttaa gaatttttac tgcttttaat ctaccttctt
    10141 ctgttttaaa ttcttcacat ctggaagatt ttaatatagt tcctcccctg tgtataatat
    10201 ctccaacaga agataagttc atttcagtta aatcttcttc tagtaatccc ttatatcctc
    10261 tattgattcc atatactttg catccataat atattgctga tcttacaacc gctctaatcg
    10321 cagcattcat tcctggagca tctcctccac ttgttagtaa tcctatagtt ttcatttttt
    10381 acctccttgg attttatgtc aattttttat tataatcatt gttattgtat tctaatggac
    10441 tttttttgtc cctatatcac tttttaatcc catcaagact aaaaaaatta aatgtcctta
    10501 aatgtccatt ttgtgatatt atatcacaaa acggacaata tttttcatat tattataata
    10561 tatttagatt tttatataaa aaatcaagct tttactttta tgttatcttt aggaagtaac
    10621 tcttctaagt tatttataaa ttcatcacat atacaagtca atgccccggt cattttatat
    10681 gctttacgtt taccatttat attttcatcc atagggtaca agtatatact gtcattacct
    10741 ggatattgtt tggctatttc tacaatatta ttagttgtat cagtatcaga catactatct
    10801 attttcaaat acaatttttt accactttct ggcttaatat tttctacatt tctagttctt
    10861 cctgaattgt agtttctatc tgcaaagtca gtatcatcat ttatatcttt tatctctcta
    10921 gctataagtt ttgcattttc gccttctttt atacttaatg ttccttttac atatattata
    10981 ttatcttcat tcaaaatgac attatacttt tgtaataatt gaggaaaaac tattatttct
    11041 atagcaccat acaaatcttc taattcaata aatgccataa tttcatttcg ttttgttgtt
    11101 ttaatagttt tagttacaat cattccaccc attataacat ctgtttcatt catttgtagg
    11161 tatgactctt cttcctcttt taatgaattt aattttccat tatccataga tgttttttct
    11221 tgtagttctt tttcaaattg tgataaagga tgtccactta catacattcc caagacttct
    11281 ttttcaaggt taagtctctc tctttcttca tactcttgcc ttttaggtaa actgtatatt
    11341 tgttgaaaat cactatcttc ctcactatct ccaaaagcat caaataaaga aatttgacct
    11401 tgaacatttt tctttctatc catagataca ctttctaata aactttcata accagccatc
    11461 agactagctc tattttcact tatattatca aaagctccac attttattag actttctatt
    11521 actctcttat ttgtatcttt ttggtctaat cttttgacta aatctactaa atctacaaat
    11581 aatccattag attctctttc agcgattata ttgtttatta tatttacacc aacatttttt
    11641 acagctgcta gaccaaatct tatattgtcg ccttcaactg aaaactttga aaaacttttg
    11701 tttatatctg gaggtaatac atctatttct agtgctttac attctcttat atactcaact
    11761 accttatcag tatttcccat tacactggtt atcaatgctg ccataaattc aactggataa
    11821 tatgttttta gataagctgt ctcatatgct aaaaccccat atgcagctgc atgtgattta
    11881 ttaaatgcat atctagcgaa gtctatcata tcatcaaata ttttattagc tatttcctct
    11941 tctacaccat ttctgataca ccctgcaatt tctatattac catcttcatc aaatttacca
    12001 tgaataaaat attgtctttc ttcttccatc acgtccatct ttttcttact catggctctt
    12061 ctaactaggt cacttcgtcc gtaactataa ccacctaaat ctctaacgac ctgcataacc
    12121 tgctcttgat ataccaaaca accatatgta acttccatta taggctttaa tttttcatgt
    12181 agatatgtta catcttttgg gttgctttta ttttttatat atgcaggtat agaatccatt
    12241 ggacctggtc taaataggga tattcccgct acaatatctt caaaattgtc tggttttagc
    12301 tgtttcataa aacttctcat gccagcactc tccaattgaa acacccctag tgtatttcct
    12361 gatgaaagca tttcatatac tttcgggtcg tcataatcca tctttgaaaa gtctattttt
    12421 tctgtgtagt tttttataac tctatttttt tctattaaat caagagtatc tcttataact
    12481 gtaagagttc ttagcccaag aaaatccatc tttaaaagcc caagttcttc aagtgttgtc
    12541 atagtaaact gtgttgtgat tgcatcttga tgtttgtaca aaggtacata ttcatctacg
    12601 ggattttttg atataacaac tcctgctgca tgagtagaag catgtcttag cattccctct
    12661 atctgttttg atatatttat tatttctttt gtttccgggt cttggtcata caattcttta
    12721 agattaggat ttgtatctag ggccttatct attgtcattc ctaaagcaaa tggtatttcc
    12781 ttggctacct tgtcaacttt attgtagcct atatccaata cccttccaac atctcttatt
    12841 gcagcttttg cacccattgt tccaaaagtt attatctggg ctacatggtc atcaccatat
    12901 tttctcttta cataatctat aacttcttct cttctctcat aacaaaaatc tatatctata
    12961 tctggcatgg atatacgttc tggatttaaa aaacgctcaa ataatagaga gtactttatt
    13021 gggtctatat cagttatctt aagtgtatat gctactatcg aaccagccgc actacctcta
    13081 cctggtccta ctataatatt attttcttta gaaaaattta taaaatccca aacaattaaa
    13141 aaatactcta cataacccat tttttcaatt acatctaatt cataatttaa tctctctagt
    13201 atctcattgc taggattatc atatctctct tttaatccat taaaacacaa ctcccttaaa
    13261 taagtttctg gtgtatatcc ttctggaaca tcatacttag gaagatgtat agtattaaaa
    13321 tcaaactcca cattacacat atcagctatt ttcacagtat tgtcaatagc ctctaatgcg
    13381 tatggaaata attcttccat ttcttctctt gattttaagt aaaactcatc actaccaaat
    13441 ctcattctat ttgggtcatt taatgttttt cccatttgaa tacacataag aacatcatgt
    13501 attttcgaat cttctttatt tacatagtga acatcattag tagcaactag tggtatacca
    13561 gtctcttttg ataattttac taaaccagag tttacttctt tttgctcagg taaattgtga
    13621 tcttgaatct ccaaaaagaa attttcttct ccaaatatgt ctctgtagtc aagagcaaat
    13681 tgttttgctt tttcataatt tctattcatt aaagcacgtg ctacatctcc tgcaaggcat
    13741 gcagaaagtg ctataatccc tttgttatgt tttttaagtt cttccatgtc aactctaggc
    13801 ttatagtaaa aaccttctac gtatgaagtt gaaactattt ttattaaatt tttatatcct
    13861 tccatattct tagctaacag aactaaatgt ccttgatatt tgtcatagtt tgggtcttta
    13921 tctctcaatc cacgtgcagc tgtatagact tcgcacccta taattggctt tattccttct
    13981 tttatagctg ttttgtagaa atctataaca ccaaacatac aaccatggtc agttatagct
    14041 attgagctca tgtttagttc cttagccctt tttataagtt ttttcaccct cgaaaaccca
    14101 tcaagtaaac tatactcagt atgtacatga agatgagcaa aatctttgtt cattaaatca
    14161 cctcctgctt tataatttta attttaccac aaatatatgt cctaaaacaa aaacagagac
    14221 ttttatctca aaataggaat taacttcaac atataccatt atatgttata aattaatgct
    14281 tattctgaga ttgtcccctc aatttattgc tatgattttt ttttcaaatc tgttgcaatt
    14341 tcttctattt ttctaagtct atgattgact ccagattttc ctattggtgg ttctaacatc
    14401 tctccaagtt ctttgagagt catatcctca tactcgattc taatcttagc tatctcttgt
    14461 aaattttctg gcaaactgct aattcctata gtctcttgta tgagcttaat attttctact
    14521 tgtcttactg ctgcatttac tgttttagaa aggttagctg tctcacaatt tactatcctg
    14581 tttacattat ttctcatttc ttttactatt ttagtatttt gaagacttaa aagagcgtgg
    14641 tgtcctccta taatactcaa caaatctgat atttgttcac tctctttgat ataaacaacg
    14701 tgattatttt ttctagccac aattttacta ttaaaaccta aagagtttat aagttctttt
    14761 agagaatctg caaactcctc attattagtt acaaattcca tatgatagtt cttttctggg
    14821 tcgcttatag aacctccacc aagaaaagct cctcttatga aagctctctt acatccttca
    14881 tgcttaatta agttctctga aattttgttt atagtaaaaa agccttcttt atcttcaagt
    14941 atacctaact tttttaataa catttctgaa cccatttcgc ttgttaccat taatacataa
    15001 ctgttatttc tctttagcat ttgattctta tttactgaga ttgtagtatt gatattaaaa
    15061 ttctttttta ataatttaaa tactttacgg gctattgaat ttaactctgt tgttatcttt
    15121 agatttagct tcttatatcc aacgatttgt atacttcccc ctgatttaac tagagctgat
    15181 aattcagcta tattacaaca ttcattccca gatataactc ttgctagttc atttttggtt
    15241 tcagttgaaa aagacatatc tatcacctat taactaattt ttctttaacc ttttttttat
    15301 ttcttgtaag gcaaccttta ttatctcctt ttcatcatca tacctagcta ggtctaccac
    15361 tctatctaga tggataaatt tagcatcaat aaagccttcc tctttttgtg gaatagtatc
    15421 catattcttt gcttgcatca aataccaaaa aactgtttta tgaactgctc tattttcatc
    15481 ccagttttct ttgaatgtat agtgtatttc acctaaatat ttcaatatat ctgcttttac
    15541 tccagtttct tcacttactt cacgtaatgc tgcctcttgg ttattttccc cttcttctac
    15601 ttttcccttg ggtaacaccc aatctccatt gaacttcctt agtaaaagaa tcgtattgcc
    15661 aaacaggact acaccaccag cactaacctc ttctctcatc gatgttcact ccttaaaact
    15721 aacataatga taaggatttc tacacaattt tttaaaatcc ttttttacta acttttgttg
    15781 taattatgat ttagggctaa ctcaataact atgttagata tatactttgc atcatgtctt
    15841 atataattat tttttatttc aattaaattc ttctctacgg tttttatacc catctctttt
    15901 aatttatctt tttgcttttt atctaacaag acttgctctg ctccatcttg tttatataag
    15961 tcaagcattt cttctggcaa tatctcatta tttgcgatta cataatctat taaattctca
    16021 tcagtgtgct ttactatagc attaacatgc tctaatacat tgtacccttc tgtctcacct
    16081 ggttgagtca ttatatttgc tatatatact tttggagcta cagacgattt aatagcgtct
    16141 acaatcccct ctacaagtaa attgggtatt atactcgtat ataagcttcc agggcccata
    16201 attataatgt cagcatcgta aatagatgca ataacatcct ttaatggttt gacatctttt
    16261 ggatttaaaa aaattttatc aatagagctt ttttgaattt ttgactcttc aggtatacta
    16321 gattctccat ttattatatt gccattttct aatttggcaa ctaaatttac atcttccaaa
    16381 gttactggta aaactcttcc tgttatagca aaaatttcac ttaatttata tacagctttt
    16441 tcaaagtttc catacaaccc attcatagca gccaaaaaca aatttccaaa actctgacct
    16501 tttaaaagcc cttctgtaaa cctatattgc ataacttcat tcattgttgg ctctatattt
    16561 gcaagtgcta atagacagtt tcttatgtct cctggtggga gcattcctaa gtcttctctc
    16621 aatacacctg aacctccacc atcatcagca acagttacaa ttgcagttat attttgcgta
    16681 gtgtgtttaa gacctcttaa aaatactgat tgaccagtac ctccacctat cacaaccaca
    16741 tttggttctc tattttcaac aatagacctt tgaaatagtt ttactttttc attttttgct
    16801 tttttatata taaataacga tacaattaat gcaatccaac ctaaaattcc tacaataata
    16861 agtaatttag ccatgtttgc ctccattatc gcaacataga gtctctatgt ttctttacaa
    16921 ctctatatcc cttattagat aaatcctccg ctataagatt tgttatagta actgacctat
    16981 gtcttccacc tgtgcatcca acaccaatta ctagatgttg ttttccttca tctatatatt
    17041 gaggtactaa aaaatgaatc atatctaaaa gtttcacata aaattcttca ctgattttag
    17101 aattcataac ataatctcta acttctttat catcaccagt tttagctctt aactcttcaa
    17161 cataataggg atttggaaga aatcttacat caaatactaa atctgcatca gcaagtatac
    17221 catgtttaaa tccaaaagat actacagaaa tagttaggtt tggattatct tcaccagaag
    17281 aatatatttt ctttatttct tccttaaggt ctttaggttt catgttagtt gtatcaatta
    17341 tacatgtaga taactccttt aaaggttcca ttatctttct ttccattttt ataccttctg
    17401 gaatctgcat atcttttgcc aatggatgat ttcttcttgt cattttatat ctctttaaca
    17461 atacatcatc attacagtcc aaatatacca tttcatattc ataattttct ttttctaaat
    17521 aatttaagct ttcatgaagc gcttcaaaaa actttctccc tcttatatct atccctagag
    17581 caactttatc aatacttgaa tttggttgat aacataattc tgcaaattta gttatcaaag
    17641 caggaggtag attatctaca caataaaatc ccatatcctc taaagctctc attgtctcac
    17701 tttttccaga tcctgaaaga cctgtaacta taacaaattt cattgctaac tcctcccttt
    17761 tatcttatgt ttattatatt ataatgtttt ctactaacga tatatccaga ttagagtcag
    17821 atatagtctt catagctagt ttaaaatcac caactctttc tggacaatgt gcatctgagc
    17881 ctattataaa tttattttct agccctttta ttttatttaa ttgatttaca ttcaaaaaag
    17941 aatggctact atttatttca agtctagtat ttgtcttttt tgctgtcttt gctacttctt
    18001 ctatatacac atcgccttta tctcctggat gagtaattat aaaaatatca ttattactta
    18061 tcgcatttat tacagctttt gtattgtatt cttttgcttt ttcagtttta aaaatatagt
    18121 tattacaatg atttaataag cttcttaatg atgttggagt agaaccaaaa tgatatccag
    18181 ccataatata atcaaaatct ctaataaatt tatcatccat atctatattg cctttatcat
    18241 ctaatatgtt acactccata cctaaaagta tatttatatc attgtactta tcatttaaat
    18301 aatctacttc ttctctcatt ttgagaatat cttttttctt tacaccatat gcaatatgtt
    18361 tatagctatg gtcagatatt cctattgttt ttattccttt agaaatggct acctttacat
    18421 tatcttcaat agtccctttg ccatgactat ataatgtatg tgtatgataa tcagccagta
    18481 ttttcatttg ttaatcctct ccaagtattt tgacttcttc ttccaacata accccaaact
    18541 tggcatatac agcacttttt ataacatata ttaaatccaa aatatcttta gcacttgctt
    18601 ctccttgatt gactacaaat ccacaatgct tctcagatac ttgtgctcct cttaaagtaa
    18661 gacctctcaa tccagtttct tctattaatt tacctgcaaa atatcctgtt ggtctcttaa
    18721 atgtacttcc tgcactcgca aaatttaatg gctgtttagt tattcttctt gtcgctaaat
    18781 catccattaa actttttatt tcatcataat ttccttcttg taattctagt tctgctgata
    18841 aagctatata tccgtttttc gatattatac tttttctata cccaaattcc atctgttcat
    18901 ttgataattc aaatatattg ccttccatat ccatcagtct tactgacttc actatgtcct
    18961 tcatttcacc accatatgct ccagcattca tggctagtgc tcctcccaat gtaccaggta
    19021 ttcctgatgc aaactcaaac ccttttaatt cttgcttttg gagagcttta cctaaaactg
    19081 ataataaagc tcctgactga gcagtcatcc tagtatcatt tatctcataa gaattgaaat
    19141 tatcagctaa ttctattacc actcctctta ttccaccatc tttaattaaa atgtttgaac
    19201 catttccaat tattaaatac ggaatagatt cttcttttac caacttaagt acatttttta
    19261 attgttcttc agttctaggt ctaactaata tatctgctgg tcctcctacc ctaaatgaaa
    19321 tatgtttttt cataggttca tcgactttta tatcctcttt acttaatata tttaataaat
    19381 ttatatatat atcttgatta ttcattgtat ttcctcccaa attatactga actttagctg
    19441 tcattattat ttaaattaca ttagtatata gtattaacta tattcaataa tatatgattt
    19501 tgcttatcat atggatttta tattttaagc ttcttaatta caaactatat tcaataaata
    19561 tataataaag taaaaaaaaa aaaataacaa catataaaag aatataaata atttaaaaaa
    19621 agcatcaata tcactgatat caatgcttta aaaggcaatt taattcgtat tatttatcta
    19681 actatttttg acatagcaaa gataataaat ccctccaact actatggaac ctccaacgat
    19741 atttccaagc gttacaaata ccaaattagt tataaattga accatagtta tatttgctcc
    19801 taatgccatt cccattggta taaaaaacat gtttgcaaca ctatgttgga atccgcataa
    19861 tacaaataac attattactg tccaacatga agccaatttt gacattatat cttgagatgc
    19921 tgtagccatc catacagcta aaacgactaa aatattacaa agtattgctt ttaaaaacat
    19981 tgatgctata gttgaattaa ccttagcttc tgccacagct atagcttttg tagctgcatc
    20041 tcctgtcaat gtagaagaat aaaacacaag tgcaactagc agaattgaac ctactaaatt
    20101 tgcaaaataa accagtcccc agtttcttaa catttttcct aatgttattt ttttgtccat
    20161 aagtgcaata gccattaagg tgtttccagt gaataattct gctcctgcca ccactacaag
    20221 cataagacct actggaaaga ccatagcact taaaaagcta gctacccctg ggtctatctt
    20281 gccaaaagtt tgactaacta tcatactccc caatgcccca aaacctataa atgctcctcc
    20341 aaataaacct aatagaattg cattttgtgt cgaaatgttt gcttttttaa tgcctgcact
    20401 tatagttgct tgtgcagttt ccccaggtgt caagaatcct ttactcataa atccttcccc
    20461 cttaaaaatt atactttccc ttgtcttttt atggcaataa ttatataata acaatatgat
    20521 atataataaa ttttattaac ataaaaaata tactatatac ttatatatac ttgtctcata
    20581 taattatact atttattata taacaacttt gttaatttcg aaataattta tattgaattt
    20641 gaaaaattta ccttttgatt atttatttta ctttttaatt attgaggtat tatatataat
    20701 agatatatgt ttgtgttatt atatataatt acttatttta ataaatattt gaaggaggga
    20761 attttatgtt tgatatatca tttttcgaaa tgtcaatata tgaaatagca gttactacaa
    20821 tatatataat taatttcatg gttatactaa accttatctt tagagaaaaa agaaacataa
    20881 atacaacttt aacatggctt cttatattag tattgattcc tgctcttgga ttcatacttt
    20941 atatagcttt tggcagaaat atttcaaaaa acaatatgtt tagattgaaa gaaaaggatg
    21001 ataagataat caaaagtaat atactagata ctcaggttaa actccaatct acatctgaaa
    21061 ttgattcaga tatacatcaa cataaggata tgatttatgc attggctaat tcaaataatg
    21121 ctcattatac caataataac gatgtgtgga tttatgctga atctagtcaa ttttttaaca
    21181 gtcttcttga agaacttaaa aaagctaaaa aatatataaa catacaattt tacattttca
    21241 aagatgacaa aatcggaact gaaataatag acattcttat agacaaagct aaagaaggtg
    21301 ttgaagtaag acttttattt gatgcagttg gtggtagaac actaaaaaat agtacacttt
    21361 ccagacttaa agaaagtggt gttaaagttg gaagcttctt cccatcattc ttgaaaattg
    21421 taaactttaa cttaaactat agaaatcacc gaaaaattgt tgtcattgat ggtaaggtag
    21481 gatttgtagg tggatataat gttggtgatg aatatttagg tagaaatcca aaatttggtc
    21541 tatggagaga tacacatact aagcttactg gtgattgcgt aattgaccta aatatgagat
    21601 tcatccttga ttggagatat acaactaaag aagatttaga tttagaaaag tactttatag
    21661 aagaagatac aaattctaat catccttctg aaaatattgg gattcagata gtatcaagtg
    21721 gtccagatat atcagaactt gatgagatta aatatggtta ccttaaaatg attcagaaag
    21781 ctagaaagta tatatacata cagagtcctt atttaatact tgactcaact tttatagata
    21841 ctttaaaaat agcttgttta tctggtgttg atgtgagagt tatgattcct tctaaacctg
    21901 accatccctt tgtatactgg gcatcatatt catatgctgg agaactttta aagtttggag
    21961 ctaaaatcta cacttatggt caaaatgcat ttttacatgc taagacaata gttatagatg
    22021 actctatatg ctctatagga acagctaata tggatatcag aagttttgag cttaatttcg
    22081 aagttaatgc atttatgtac tcttcaaaaa aagcattgga acaaagaatt atatttgaaa
    22141 atgatatttt aaattctaag gaaataacat tagatatata taattctagg tctacttata
    22201 taaaaatcaa ggaatcaatt tcaagactat tatcagcagt tttataaatc ttttatttta
    22261 taaaaatttt acattttggt ttatttttta aagtttttta tatgtattat ttttttttta
    22321 ttgaatttag ctcatttttt ttattttttt taactattct gaattatttt atattataat
    22381 agactagtat agtttttatt tttaataggt aacttttttg cattgttttt ataaataaag
    22441 aaattgttgt atattattct ttctttattg ttctggtttt gttcacccac ataatgttgg
    22501 ggtcaactca tggttaatta ttttatgaaa aataataact ttaaaaagaa aagggggatt
    22561 tgttatgtgc gattttattt cacacaacaa gcaattattt gacgaactag atatatttat
    22621 tgactctctt acaacaaagg aaggtgcgtt aatacaagtt ctacatgaag cccaaggtat
    22681 atttggatat cttccaaaag aagttcaact tcatgttgct agaaaacttg gagtagctcc
    22741 tgcaaaggta tatggggttg ttacattcta ttcttatttt actacagaac ctgtaggtaa
    22801 gtataaaatc agtgtctgtc taggaactgt atgttttgta aaaggtgctg acaaaatctt
    22861 aagtgctttt gaaaaacagc ttggcataaa agttggagaa actactagtg attttaaatt
    22921 ttctttagag ggtttaagat gtttgggggc atgtggttta gctccagttg ttactgtaaa
    22981 tggtaaagtt tatggtaaag tgaaacctga ccaagtaagc gaaatattag atacttatag
    23041 agaattagaa ttgaattgtt aagaggggtg aataaataat gcgtaaagta aattcatttg
    23101 atgaattaaa agttttagct gatgagctga aacctaatct tagcttaaga aaaaattatt
    23161 ctaaggaaaa tgtattaaga agagaattac ttgtttgttg tgatactgga tgtacctctt
    23221 caaacagtct agaaatagtt agtgaattag aaaatgaaat aaagaaatca ggaatccaag
    23281 ataaagttag tgttcgtttg acaggttgtt ttggtttctg tgctcaaggt cctattgtaa
    23341 aagtttatcc tgataacgtt ttctacgtaa aagttgagcc atctgatgct gaaaagatag
    23401 ttcaaagcca cttaattaga aatacagttg tcgaggaatt attatatgag gaagaatctt
    23461 taagtaaaaa agttgaagaa caagaagaaa tgtctttcta caaaaaacag ttacgtatag
    23521 ctttaaaaaa ttgtggtctt atagacccag atagtataga agaatatatt gccaatgatg
    23581 gttatttagc tctaggcaaa tgtttaactt ctcttactcc tcaagaagtt attgcagagg
    23641 taaaaacttc tggccttcgt ggtagaggtg gtgcaggttt ccctactggc tctaaatggg
    23701 aagctgcttc aaaaaatcca gctggtccaa cagataaaaa gtttgttgta tgtaatgctg
    23761 atgaaggtga cccaggtgca tttatggatc gatctgtact tgaaggtgac cctcatagtg
    23821 tactagaagc tatggcaata tgtggatacg ctataggttc tgatacagga tacatataca
    23881 taagagcaga atatccaaaa tctatagaaa gattaaaagt agccatagct caagctgaaa
    23941 aatacggttt acttggcgaa aatatcttag gcactggatt taactttaaa ttagaattaa
    24001 aatatggtgc aggtgctttc gtttgtggtg aaggtacagc gctaatgcat tcaattgaag
    24061 gtagacgtgg tgaacctaga atgaaaactt atagttcttc taagagtggt ctatggaaat
    24121 ctcctacttg cttaaataac gttgagactt ttgctaacat acctgcaatt atacttaagg
    24181 gtggagattg gtttgccaac ttaggtactg aagattctag tggtactaaa gtctttgctc
    24241 ttggtggaaa agtagaaaat gtaggacttg ttgaagttcc aatgggtact actttaagag
    24301 atattgtatt tgaaattggt gggggaatcc ctgaaggtaa gaaatttaag gctgttcaaa
    24361 caggtggacc atctggtgga tgtataccaa ctgaacattt agacactcct atagactttg
    24421 gttcattaag ttctatcggt tcaatgatgg gctctggtgg tatgcttgtt cttgatgaat
    24481 ctgactgtat ggtagatata gccaagttct tccttgagtt tactgttgaa gaatcttgtg
    24541 gtaaatgtac accttgtcgt ataggtacta caagattatt agaaatatta actaaaatta
    24601 cagaaggtaa tggtactcta caagatttag atgacctaga aaaccttgct gaaactatac
    24661 aaactgcttc attatgtggt cttggtaaag ctgctccaaa ccctgtttta agtactcttc
    24721 aatatttcaa agatgagtat atagctcatg ttgtagataa gaaatgtcct gctggtaaat
    24781 gtcagaactt actttcttac tttataacag atgactgtaa gggttgtaca aaatgttcta
    24841 gagtgtgtcc tgctggttgt ataacaggtt ctgttaagga acaacataca atagatactt
    24901 ctaagtgtct aaaatgtggt gcttgtatag ataattgtac atttaacgct ataatcaaga
    24961 aataacttat ttggaggagg aataacaatg agtttagtta atttaactat taatggcaaa
    25021 cacgtttcag cacctagtgg aacttctatc ttagatgctg cgaaattaat aaatataaaa
    25081 atacacaacc tttgtcattt acatatgaac gaaatagata agcttgacac ttgtgcttct
    25141 tgtcgtattt gtatggttga gacagagaga ggcttagttc cagcttgtgg aacagttata
    25201 aaggaaggta tgaaagttca gactaatagt gctaaagcat taaatgctcg tagaactatt
    25261 gttgaattat tgttatcaga ccatccacaa gattgttttg tctgtgaaaa aaatggagat
    25321 tgtgaattac agactatagc tgctgattta ggagtaagaa aaataagata tcaaggttcc
    25381 aaatcttttg caggtaaaga tacttctact aaatctttag ttaaagacca ttctaagtgt
    25441 atattatgta gaagatgtga gactgtatgt aatgacatac aaacagtagg agcattatct
    25501 ggagttaatc gtggatttaa tactttagtt agtacattct ttaactctga tatggttgaa
    25561 acagagtgta cattctgtgg tcaatgtata tctgtctgtc caactggagc attaacagaa
    25621 gtagataatg tacctaaact ttgggatgtt ttaaataaaa aagaaaagac tatagtagtg
    25681 caagttgctc ctgctgtaag agttgctatt ggtgaagagt ttggattaga accagggtca
    25741 atctctacag gaaaaatggt agctgcttta aaagcacttg gatttgaaca tgtatttgat
    25801 acaaactttg gtgcagattt tactataatg gaagaagcta ctgaatttat agaaagaatc
    25861 caaaaaggtg aaaatcttcc tatattaaca agttgttgtc ctgcatgggt aaacttctta
    25921 gaacacaatt atccagacaa gttaaactta gcatctagct gtaagtctcc tcaaggtatg
    25981 tttggttcta tagctaaaaa ctactatgca cctaaaatat taggaatcaa tcctgaagaa
    26041 ctttatgttg tatctgtaat gccttgtgtg gctaaaaaat atgaagcttc aagagaagaa
    26101 ttaagtgaat ctggaatact agatgttgac ctttctataa ctactagaga gttagctaaa
    26161 atgataaaag aagctgctat cgatttacct aatcttcaag accaagattt tgataatcct
    26221 ctaggaaaat ctacaggtgc tgcatctata tttggagcaa gtggtggagt tttagaagct
    26281 gccctacgta cttcatatga gaagataact aataagactt tagataatgt aaacttcact
    26341 aatgttagag gtcttaaagg aattagggaa gcaagtatag atgttgatgg aactacagtt
    26401 aatgtgtgta ttgtaaatac acttaaaaat gctagaaaga ttatggataa ggtcagaagt
    26461 ggagaatgta aataccatat catagaagtt atggcatgtc ctggtggatg tgttggaggt
    26521 gctggtcaac cttatcacca tggtaatact gaaatagttg atagacgtgc aaatgcgtta
    26581 tatgaaatag atagaaataa ggctattcgt aaatctcatg agaatcctga tttacaagct
    26641 atttataaag acttctttgg agaaccaaat agtgatgtag cccataaata ccttcatact
    26701 cattactttg ataagagttg tgtctatgga gagtgtccac aagaatgtgc ttgtgaagaa
    26761 gctaagtagt cataacaaag tataaaattt attcttaaaa aaggattgtc ttaaaatact
    26821 tttacgtaat ttaagacaat ccttattaat ttcacaaatt aaattatatt tctaaataga
    26881 cttattcttt aattaaccta tctattaatt aaagaatacc tgccattctc ataagttgtt
    26941 ttgcatttgt agttttacat cttccatctt ttatcttata gtcaaaatga attttatcgt
    27001 ctacatagta ctctgagaaa tgatagttta caacttcaac atctccacta cctgaaaggt
    27061 cacatagctc aaagtcatgt gttgtcacca tacttataac ccagggtttt gataatttct
    27121 ttactgcctc acttgctcca ataattctat cagcagagtt agttccctta aaaatttcat
    27181 ctactagaac caacattggc agttctttta aactatattg aatcattgat tttattctaa
    27241 gaatctcagc atagaaagtt gatattcctt gacttacatc atcttgtatt ctcatagaag
    27301 taaatatagc catacaggtc gcatcaaaac tcttagcgca tactggagca cctgcatatg
    27361 ccagcactaa gtttacaccg atacttctta aaaatgttgt ctttccagac atatttgaac
    27421 ctgtgataat acaagtctga gaatttaatt gtatcgaatt tgctatgaca cttttttctg
    27481 ctattaatgg gtggtatacc tcttcaactt tcaatcttgg agtatcttca tattttatta
    27541 ctggaaaaca tgtattttcc tttacatgag aaataactgt taaacttatt agagcttcaa
    27601 attctccaat agattcaaac caatctctaa tttgatttcc atatatacct ttccatcgct
    27661 ccaaagcttc tgcacaatga tagttccaca tcaaaacacc acaggcaatt atatatagta
    27721 tctgattata ccttaggttt acagcatttc ctattgaatt taattgttta acacctcttg
    27781 aaactccact tcccttagaa agtttatctt gtaattcttt caaatatgca ctctcaaacg
    27841 tctctttttc taaaacctca aacatttttt catatacttg tatacttcta ctaaatgaaa
    27901 ataatggaga aagagtttgc ataagtcttg gatatccaaa accactaaac cctaactgga
    27961 ttataaaaag taaaaaaaca ggtaaaacag gtaaaaatcc taacatacaa aatataaaag
    28021 ataagattgt tgcagttggt agaccccaag taaaaatatg catccatttt ggtatataca
    28081 cattcttatt ttctccatac tctataaacg attctatact actatctgaa ttattttttt
    28141 gctctttacc aattatatta ctgagtattt gaatatgaat agaaaaatca tcttttgata
    28201 aaagttcttt tatagccctc tgtctttcta agattacatt ctcatctgga ttttcattcc
    28261 acaaatattt ggctaaaaaa tttttcccat aagatgtatt agctacacag atatattgat
    28321 acaatgatgc tcttccaaat aagtctaaat cttttgcttg cgaattttca tcttttaagt
    28381 attctttacc atctatttta aattccttcc aaccatctcc aaatctctta atatacttat
    28441 ttaatacttc gctcttactt ttaaaatatg tttcttcttc ttttacttta ctatgaataa
    28501 ctaagagtgc tataaatatt attattgata aaactccaac aaaaatacct actatatttt
    28561 tattgtaaaa tccaatagca aaccctgcta tcactatcaa aaaagatatt aatcttgctg
    28621 tacttatgtt atttgacttt ttacttaaga gcaattcttt ttcagtacta ctttctaaaa
    28681 gtttactaaa atattctatt cccataaaaa attccaactt tcttgttata taatgaaact
    28741 caaatataaa attaatctag tattattata atctaacact gtactttaaa ttatccttat
    28801 ataatatatc ttattaataa acattatata tatacaactt gattttaatt tataaggtgt
    28861 aggacataat cctaatcaat atttaattaa atgatgtgta gttcactatt attcagtact
    28921 acttagtatc gattaatttt gataattttt tagtaaattc ttgagctgca tcgtatccca
    28981 ttcctctttg tctaaaattc attgctgcta cttctaatat catagcagta tttcttccag
    29041 gtttaacagg tacaactaat ttctctacat taacaccaag tatttcttcg tattctttat
    29101 ccagtcctag tctatcataa tacttagttt catcccaagc ttctaaatga actactaaat
    29161 ctatcatttt agaatttttg attgccccta ctccatataa acttctaaca tctattattc
    29221 ctattcctct tatctcaaga aaatgtctta ttagttcagg tgactgaccc acaagcatac
    29281 tttcatctaa ttttcttatt tctacagcat catctgcaac taatctatta cccctttgaa
    29341 tcagttctaa tgctgtttcc gactttccta tactactttc accttttatc agtacaccta
    29401 taccataaat atctactagc accccgtgaa ttgttgtgtg tggtgctaac ttattatcca
    29461 aatagtttga cagtctattt ataagtctag tggtatttct atttgtgctc aaaagtattc
    29521 ttttatattt tttagccttt tcaattacat ctggcttgat ttccaagtct ctagaaatta
    29581 ttaacgctgg tatttcatat gagaaaaatc tatctagtac ttcttctcga atatgactat
    29641 ccattaatcc aaaaaaatta tattctgtct ttccaactat ttgtattctt tcatatgcaa
    29701 aataatcaaa atatcctgca aactgtaatc ctggtctatt tatatcttga gagtagacat
    29761 aataatctac atcatcaggc atgtatacta cttttaactt caactctttt attatctctc
    29821 ttattgatac tttattgcta gtttccatta attttcttcc tttctaaaaa aattataaac
    29881 attatctgca atgcttttat tcattccatc tacttctaaa agttcttcca tagtagcttt
    29941 ttttatagcc tctacatctt taaagtgatt taataatgat ttctttcttt tttcacctat
    30001 tccttgtata tcatctaaga tagattttgt caaagcttta tttcttagac ttctatgata
    30061 tgttattgca taattatgaa cttcttcttg tatactagca ataaatctat ataaattagt
    30121 tgttttatct agttctattt ctttttcttt gcatatcaat cctttagttc tatgcttatc
    30181 atctttgtac attccccaaa gaggtatatc tacatcattg agttctagta ctttttttac
    30241 tgcactaact tgtccttttc caccatccaa caatatcaag tcaggtaaat ttccatgttt
    30301 taatcttcta tctacaattt cagccataga atcataatca ttaggtccaa taactgtctt
    30361 tattttatat ctcctatatt ccttcttatc ttttttagca ttcgtataca ctaccataga
    30421 accaatcgaa tctacacctt gtatatttga tatatcataa gcctctattc ttattggcaa
    30481 cttttctaga tttaatattt gttttaattc ttccaatgct ccaatacttt tttcatattt
    30541 tcttttattc atatcagaaa atttttcaag atattctaca gcatttttcc taaccatttc
    30601 aactaagctt ttcttctctc ccttttgagg aactcttatt gttacttttt gccccttttt
    30661 agatgacagc cattcttcta atatagaact atcttctatc tcatcttcta taattaattc
    30721 tttaggtata tactcttgtt cattataaaa ttgttttaca aatgaactta atatagaagc
    30781 tcttggacta tccattacac cttctaatat aaaatgttcc ctacctacaa tcttaccatt
    30841 tcttataaaa aatacctgga cacaagcttc attatgagct cttgccattg caaccacatc
    30901 ttggtttaag tcacttacag tagcatctat tttttgtttc tgtatcatct cttcaaggct
    30961 ctttatctta tccctatata cagcagcctc ttcaaatcta aagttcatag aactctcatt
    31021 cattttttcc tttaaaagtt ccatcaattt ttcttcttta ccagataaac acattataat
    31081 ctcttctatc atttttccat actcttcttt agaaacattt cctgtacaag gtcctaaaca
    31141 tttatttatg tgtagattta aacaaggtct tgtcttattc tttattgctt tatctatatc
    31201 tattttacag cttcttattg gatacgtact acttataagc tccaaggtat cattcacagc
    31261 agttatattg gtatagggtc caaaatactt tgccttatct ttaagaactc ttctaacttt
    31321 taagattctt ggataatctt cattcgtagt tacttttata tatggataag ttttatcatc
    31381 tctaagcact acattatact ttggtctgta tctttttatt aaattacatt caagtattaa
    31441 tgcctctaac tctgaatccg ttataatata ctcaaactta tatatattct taaccataga
    31501 ttttacttta gatgtatggt tttttgaaga ttgaaaatac tgccttaccc tattttttag
    31561 agatatggct tttcccacat atattatatg gtcgtatttg tctttcatta aatatactcc
    31621 gggctcagaa ggtaattttt ttaaatgctc ttgtatatca aacatctcgc cctccaaaac
    31681 attaaatttt atactaaaat tataccctaa aatatgaatt ttaaaaagtt atacaatttc
    31741 cagtgcttta gacaagttaa agtccatatt cccttctata attgatttga aaacaatttt
    31801 aaccatgaag agtaatatgg actttacttt gtattactat ttacaactta tatttgacta
    31861 atttacttat aacaaaacag tttatcgtat atctgctcta aaatttttca caatttatat
    31921 aacttaagaa aaatatttct ttaagaattg acctgtataa gaaccatcaa ctttagatac
    31981 ttcttttggt gttccagttg caactatagt tccaccctta tctccgcctt ctggtcctaa
    32041 gtctattaca taatcacaag tctttataac atctaaatta tgttctatga ctataattgt
    32101 atttcctgta tctgctagtc tttgtaatac ttggattagc ttgtctacat ctgccatatg
    32161 aagtccagta gtaggttcat ctaatatata caaagtcttt cctgttggtc ttttactaag
    32221 ttcagcagct aattttatac gttgcgcttc tcctcctgat aattgagttg aaggctgtcc
    32281 taatttaata tatgaaagtc ctacatccat taaagtttct aattttcttt ttatatttgg
    32341 tatattttcg aaaaactcaa cagcttcttc cacattcata tctaatatat cagatatagt
    32401 tttatctttg tattttactt gcaatgtttc tctattgtat ctttcacctt tacaaatttc
    32461 acaaggaaca tatacatctg gtaagaaatg catttctatt tttattattc catcaccctt
    32521 acaagcttca catctaccac cttttacatt aaaactaaat ctaccttttt tataacctct
    32581 tgctttagct tcattagtag atgcaaaaat atctcttata aaatcaaata cacctgtata
    32641 agtagcagga tttgaacgag gtgttcttcc aataggactt tggtctatat ttataacttt
    32701 atctatactt tcaattcctt ttatctcctt atgcttacca gctctttgct taagtctatt
    32761 cactttactt gcaacacctt tgtaaagtat atcgtttatt aatgtacttt ttccagaacc
    32821 tgaaacacct gttatgcatg taaacttacc taatgggaac tttacatcaa tatttttcag
    32881 attattttct gatgctttaa ctatttctat aaaacttcca tttccttctc tagtagtctc
    32941 aggaagcaat atttgttttt taccacttaa atattgaccc gttattgagt tctcattttc
    33001 aattatttca tctaaagtac cctgtgcaac tatttcacca ccatgtatac ctgcacctgg
    33061 accaatgtct ataacataat cagcttctct cattgtgtct tcatcatgct ctacaacgat
    33121 taaggtattt cctaaatctg ttagatgtct aagagtagtt ataagcctat cattatctct
    33181 ttggtgaagc cctatacttg gctcatcaag tacatataaa actccaacca atgcagaacc
    33241 tatttgagta gctaatctta tacgttgcgc ttctcctcct gataaagttc cagcttttct
    33301 tgataaattt aaataatcaa gacctacatc tcttaagaac atagctcttc cttttatctc
    33361 ttttataatc tcatgagcta taaatctctg tttttctgta agatttatat tttccataaa
    33421 gtcaatcaat tcatttacag ataagtttgt aacttccatt atattttttc catcaactaa
    33481 aacagataaa acttcctttt taagtctcat ccctttacac tttggacatg gtctctctgc
    33541 catgtattct tcaatcttat ctctcatgta atctgaatta gtttctctat atcttctctc
    33601 taagtttact attacacctt caaagtatga tttataattt ctagttcctc caaattttga
    33661 ctcataagta aactctacaa ttatattatc tttaccataa agtaattcct ccacaaaatc
    33721 atctggtaaa tctttaaacg gtgttgtcaa agatacatta aaatgctctg ctaaactttt
    33781 tatcatttta ctgtaataag tgtcatcatt aacacctgtt gaaccccatg ctgccactgc
    33841 accttgtttt attgataaat ctttgtttgg tactactaat tctgggtcaa cttctttact
    33901 ttccccaaga ccattacaaa catcacaagc accaaaggga gcattaaatg aaaacatcct
    33961 tggagataat tcttctatgc caattccatg ttctgggcat gcaaatttag ttgaaaaaag
    34021 aatttcttct ccatctatta tttgggctat tacaagacca tcagatagct taactgcagt
    34081 ttcaattgaa tctgctagtc ttgattctat tccatcttta attactattc tgtccactac
    34141 aacttctata ttatgttttt gatttttatt taagattata tcatcactaa cttcatagtt
    34201 ctcaccatta actctaactc ttacaaatcc ttcttttttg atattatcaa agactttttt
    34261 atgtgtacct ttttgtcctc taacaattgg tgaaagaatt tgtaatttcg ttctatctgg
    34321 aaaatctagc attttatcaa ctatttcttg aatagtcatc tgacttattt tttcaccaca
    34381 tgtagggcaa tacacatctc ctactcttgc aaataatagt ctcaaataat catatatttc
    34441 tgttactgtt ccaaccgtag aacgtgggtt ttttgaggta gtcttctggt caattgagat
    34501 agctggtgat aaaccctcta tatattctac atttggcttt tccatctgac ctaaaaattg
    34561 tcttgcataa gcagatagac tttctacata tcttctctgc ccttctgcat atattgtatc
    34621 aaatgccaaa gaagacttcc cagaccctga caaacctgta aaaacaataa atttatctct
    34681 tggtaattct aaatctatat tttttaaatt atgttctttt gctcctctta ttataatttt
    34741 atcttccata atgttgtttt tacctttcta tttttccaat ttttcttcta attgtttcac
    34801 tttatctcta agttctgctg ctctttcaaa ttgtaagttt tgagcagctt ccatcatctc
    34861 tgattttaat ttatctatat tagctcttat ttcgtcttta tcatctgtat cagatattcc
    34921 atatacaact tcttcctctg caaccttagt agcttctata ctatctctta tatttttctg
    34981 tatagtttta ggaattatat tgtgctcttc attatataaa ttttgtatat ctcttcttct
    35041 ctttgtttca tcaatagcat tttgcattga gtcagttatc ctgtctgcat acataattac
    35101 tcttccattt tcatttctag ctgcacgtcc aatagtctgt attaaagctg tttcggaacg
    35161 taagaaacct tctttgtctg catctaaaat tgctataagt gaaacttctg gtatatccaa
    35221 tccttctcta agtaagttta ttcctacaag gacatcaaac ttccctaatc ttaaatctct
    35281 tattatttct gttctttcta atgtaactat atctgagtgt aaatatttaa cttttatccc
    35341 tatttcttta agatagttag tcaagtcctc actcattttt ttagttaaag tggttataag
    35401 cactctttca tttttttcta tagttttagt tatttctcct accaaatcat ctatttgatt
    35461 atttataggt cttacttcta caattgggtc taaaaggcca gttggtctta ttatctgttc
    35521 tgctattgtc tctgagtgtt gaatttcata aggtcctggt gtagcactta caaacaacat
    35581 ttgatttata tttccttcaa attcttgaaa atttaatggt ctattgtcaa gtgcagaagg
    35641 taatctaaaa ccattctcta taagtgatgt ttttctagac ctgtctcctg catacatacc
    35701 tcttacttgt ggtatagtga catgtgcctc atctacaatt atcaaataat catctgggaa
    35761 gaaatccatt aaagtatatg gtctttctcc ttctgacctt cctgtaatat gtctagaata
    35821 gttctctatc ccctgacaaa atcctatttc ctttaacatt tctatatcat acttagttct
    35881 ttgttcaatt ctttgtgctt ctaatagcct atcattttcc ttgaaaaatt taatttgttc
    35941 ttgaagctcg ttttctattt ctacaattgc cttttcaatt ctttctggcg ttgttacata
    36001 gtgagatgct gggaatataa caacatgatt tctagtacca acaatcttcc ctgttacata
    36061 atcaatttct gttattctat ctacctcatc accaaaaaat tctattctta ttgctttttc
    36121 atcattacta gctgggaata tttctagtat atctcctcta actctaaagg tacctcgtgt
    36181 aaagtttatg tcatttcttt cgtattgtat ctctattaac ctttttatta catcatctct
    36241 gtctctttgc atacctggtc ttatagataa cataagttct ttatagtctt ttgggtcacc
    36301 aagtccatat atacaagata cagatgatat tattatagta tctcttctct ctaaaataga
    36361 tgctgtagca gaatgtcgaa gtttatctat ttcatcattt atgcttgcat ctttttctat
    36421 ataagtgtca ctgtgagcta catatgcttc tggttgataa taatcatagt aacttacaaa
    36481 gtattcaact gcattgtctg ggaaaaattc tttaaactca ctgtaaagtt gtgctgctag
    36541 agttttatta tgagccatta ttagggtagg tttcttaact tgttgaatga tatttgccat
    36601 tgtgaatgtt tttcctgaac ccgttacacc taataatgtc gaaaatttct catttctatt
    36661 tatagaatcc actattgatt taatagcttc tggttggtca cctgtaggtt tgaagtctga
    36721 ttttatttta aaatccatag tatcactcct tttttcgaac attagttcct gacatgtatt
    36781 attatatcat acctttataa aattgtttaa gttattttaa atattttacc catttaaatt
    36841 ttacttaaat ctaaaacata aaaaaagact tctatctttg caatagaagt ctataattca
    36901 tggaaaataa atactttatt caacttacat aataaaatca aatgcttctt tttcaactcc
    36961 atctaatttt aaatcaaaca tttttaatat agttgctgca tgtgctctca ggtctccttc
    37021 ttctataacc acaccacctt taatatttgg tccaaaagct ataaatggag gtctattacc
    37081 tttagtaggt aaatgaccat gtgtcgcaac agcaaacttg taatctttta tatctgaatc
    37141 ttttataata cttccttcga aatcatttcc aaatgaaatt ccttcaagac cttcaatcac
    37201 catttcaaaa tcaccttcaa gaccaaggtt agatgcttcc tctttggtaa aaatattctc
    37261 tatgaataat tcttctttaa attcttcaag cagttctctt aattctgtta acctatctaa
    37321 gttatttact attatttgag ctgatattcc agaagaattt acataggcat caaaatcctt
    37381 aacttctcca ttttcaactt ttataaatcc atttgattta agcaagacat ttggatttat
    37441 aactttatca actctaagat gaccatggtc cccaagtatt ataaaatttg tatctttgta
    37501 tgtacctgca tcctttgtag cctgaataat atctcctagc catttatcat tcatttttaa
    37561 tgctttatct acttcgtcat taaataagcc ataattatgt cgtgtatggt ccaatgtagc
    37621 taaatgtatt agcattaaat ctggtttata ttcctttatt atatctactg aacagtttac
    37681 tccaaataaa tccatgttgg gttctaattt ccaatcaata taatgacaat gtctattgta
    37741 aataccgtcc attataacct tactggagct tcgttcaaaa gtttctcttg ggtcatcttc
    37801 tctactagga gcccatattt ctgcaatatt ataatctata tttggatttg ctcccattac
    37861 aggccataat acactagagg tagttagatt attttctttt gctacatcta taatagtttt
    37921 tacttttatg tcctttgaat accagtacca gtctttattt tctttatttg ggtcaaattt
    37981 ttcattatga tatataccat gttttttagg atatactcct gtaaccatac tagtatgaca
    38041 cggatatgtc agagtgggat aaacacaatt tatatttttt actatggagc aattttctag
    38101 tatagattta aagtttggta gattttttac aaattccaag tctttttcaa ataaagaatc
    38161 tactgataat actataacct ttgtcattta cattacctct tcatttatat attattgaat
    38221 acttttaatc aacttacaag tctatataat tatttatttg ttttatctat tactgcttgc
    38281 attgcagaag ttcctttgct tactacatct tttggatttg catttaaatc agtaagcccc
    38341 tttttaatct catccattag cttatcagtt gcttccttgt atccatttaa cattggaagt
    38401 gcaacagcat attgtaattg gtcagttgca accttatatt gaggtttttc tttatataat
    38461 gtttgtaact tttctgagtt tgttgcatct tcagttgttg gtaagtatcc tgttgatgaa
    38521 ctaaactgaa cgattttatc tgtttgtgtc atccattcta taaattctgc acttgcttct
    38581 ttttctttat ctgttccacc ttcaagcata actagattag ctccacctgt aggagttgcg
    38641 tatttttgat tttttggtag gaatgctgtt gatatttcaa acttaccttc tgtttgttgc
    38701 ataagtcctg ctaaatctgc tgttgatgta aatatcatac ctactttacc atttacaaaa
    38761 tcttgttttg ctacgtccca ggcattataa tcttctccag gaggcatttt cattattcca
    38821 gctttcatca tatcttgcca gaatttaact ggtgcttgtc ctgcctcatt attaaatgct
    38881 acttttttac cattagttat ttctcctcca ctttgcatta ccatagcttc atagaaccaa
    38941 atatctattg gtgtttcaaa accataagta tcttctgatt tatttgtcat tttactagcg
    39001 taatccttaa gctcttccca tgattttgga cctgaagggt caagacctgc tttagttgcc
    39061 atatctctat ttatgtataa tattggtgta cttctattaa atggcatacc aacaaactta
    39121 tcctcataat atgaatttgt taaaagacca gaaaagaatc tttcttgata tgttttatca
    39181 aaatatttac tcatatctac taaagcacct gctgatgcga atgtacctgt ttgagttatt
    39241 tctaataatg ttacatctgg ttggtcttta gatgttaagg ctgcctgtag ttttgttgca
    39301 ttttcatagt aatctttctg ttttaacata tttacttgta catccttatg ttcactatta
    39361 aattggtcaa ccattttttg agcagtttct ccattagttc cacctaaagg agcccagaaa
    39421 tctatcttcg taatattgct atcagtcttt tctcctttag atgcatttgc atcattagaa
    39481 tttgaacacc caactaaaga acctaataat aatgttattg ctaatcctgt tgctaatttt
    39541 cttttaagct tcattttcat cctccaaatt tagtctttaa ttttttattt tattcctgaa
    39601 tacataaaag cttcttttac ttttttattc gcaaatatat aaacaagaat tattggtaaa
    39661 attagaatta tatttcctgc cattattaca ttccatgcag taattccttc tgaagattta
    39721 aggagcgcaa ctcctattgg taaagttctt attgcatctg tatttgtcat tactaagggc
    39781 cagaaataat tattccaatg gtatataaaa ttaaataata taaatgttat tactgctggt
    39841 tttgccatag gaaccattat tctaaacatt atttttaagt cagaagcatt atctagttta
    39901 gctgcttcaa tagtttcatc tgaaatctgc ataaaattct gtctaagcag aaatatccca
    39961 aatgaagatg tcataaatgg aattataagt ggaatataag tatttataaa tccaaactta
    40021 ctcatcatca tatagactgg tagaaaagtt acttgtggtg gaatcataag tcctacaagc
    40081 aaaagcccaa ataacatttt ttcaccttta aatttaatct tagaaaatgc atatgctgct
    40141 ggaaccataa ctaaaaattg taaaattaaa acacttcctg ttacaaatac actgtttagt
    40201 aaatatctaa aaaatggacc tgaatgccat gctgtactat aattttctaa cactatatca
    40261 tttggtataa tagtaggtgg taattgcatc atttcttgtg gatttttaaa ggatgtcgat
    40321 agcatccata taaaagggaa tataaatatt gcagataaaa gtaatagaat taatatttcc
    40381 attgattttg atgctatttt ttttatatca actttgtctt ttttgatagg ttgtataata
    40441 tccattgtct cctccttttg gataaatctt ataatatcta ctttttcttt attgataatg
    40501 aactttcttt gataagagtc taaagttagc atatgtagca atcataacta tagctaacaa
    40561 aaacatactc gctgcactcg cttgccctat gtcatagtat ataaatccag actcatatat
    40621 ataataagat aacatgttag ttgaacccac tggcccacct tgtgtcatta tacttatctc
    40681 atcaaatact tgaaaagatg cagttgttgt tgttatcata agaaaaaata ttgttggtga
    40741 taatagcggc aatgtcactt taaagaaatt cgttattttg tttgcattat ctaatttaga
    40801 tgcttcatat atatagtttg gtatggattt aagacctgat aatactatta agatattgta
    40861 tccaagagtc ttccatattc ctattagaat tagtgacatt aaagctgaac tttcacttga
    40921 aagccacttt gatgttggta aatgtaatgt cgatagtatc cagtttaaaa agccatattg
    40981 tggctccata atccaaagcc acaatacagc tacagatact agtgaaatta catgtggtgt
    41041 aaagattata ctttgcgtta aattgtgtat tatagaattt ttatttagcc aaacagcaag
    41101 tactatggct agtgatatag atatagtcac cataaaaaaa gtataaattg cagtgttcgt
    41161 tatagtttgt ataaactctg gattctttaa aagttctgta taattgttta agcctacatt
    41221 ttcaaaggtt gaactaagaa gactaccaga tttgaagctt agagatatcg tcttaattat
    41281 tggatagact ataaacaata aagttcctat aatagcaggt gttatatata aatatgcttt
    41341 attaaattgc tttttcataa atatctcctt taattttaga atacatactt ttacatactt
    41401 ttacatcttc tattctttct ccatttttat caaaataata taaatctttt tctaatacac
    41461 ttattcttaa agttcttcca atatcaaata tatcattttc cgttttgact cttatttctc
    41521 tattaagaac agataaacaa tatatagttt catttcccaa cacttcttta gtcacaactt
    41581 ttccaaatag gctaaataca tcatcatttt ttccatctat gtttatactc tctagtgttg
    41641 ctttttctgg tctgaatcca aatttaatat cattttttat atctagtatg ttcatacaag
    41701 gactccctat aaattgagct acaaataaat tcttaggatt ttgatatatc ttctttggag
    41761 aacctatttg cattatcttt cctttgttca ttataactat cttatctgcc attgacattg
    41821 cctcaacctg gtcatgagtt acataaacaa atgtagattt tagtttttga tgtaattgta
    41881 ttaattctgt tctcatttga tttcttaatt tagcatctaa atttgacaat ggttcatcca
    41941 ttaaaaatac ctttggattt ttaaccatag ctctagcaag tgccactctt tgtctttgac
    42001 ctccagataa gttgcctggt ttcacattta aatattctct caatcctact acatcaataa
    42061 cttctgttat aagtttttct cttttttctt tttctacttt tttgttttta agaccaaatt
    42121 ctatgttttc tctaacagtc atatgtggat ataatgcata attttgaaat accatagcta
    42181 tatccctttt actagcatcc gtttttgtaa cgtcattttc ttcaattttt aacttgcctg
    42241 aagttatatc ttcaagacct gcaatcattc ttaatgtagt agatttacca catccacttg
    42301 gacctaccaa tactgtaaaa gaaccatctt ctatagtaag atttaaattt tctataactg
    42361 tatttttttt attataagat tttgttacat tttctagtct tatatctgcc atttttatcc
    42421 tcccaaaata tttaatttaa ttctacgatt tacttataga ttaataatac tcttaatttg
    42481 taaaggatat attaagacaa agtgaatata tagtaaactt attaaaataa aggttaaatt
    42541 ataaacattt tttgttaaat atactgaagt taagatgata tacagcttat gttagctata
    42601 attttctata tttttcatat tcatggtttg ctatcacaat gtgattttaa tttatgaaga
    42661 gtcatataaa actatttatt ttttatataa tttggtttga aaataaaaaa gacctctatg
    42721 ggggatagag gtctttaagt gggggagatt gagtaattta tttttgctat attaagtttt
    42781 acaattatat tatgtcccaa atttacaagg tttaaacata aaaaataaaa cttttttaat
    42841 ttttataaat ttacc
//
LOCUS       DAEPLF010000012        41628 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEPLF010000012 DAEPLF010000000
VERSION     DAEPLF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.012"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(187..942)
                     /locus_tag="KR314_000580"
     CDS             complement(187..942)
                     /locus_tag="KR314_000580"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBH0326984.1"
                     /translation="MLKVIINGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1383..2372
                     /locus_tag="KR314_000581"
     CDS             1383..2372
                     /locus_tag="KR314_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBH0326985.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMIVLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVINATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(2643..3359)
                     /gene="dapD"
                     /locus_tag="KR314_000582"
     CDS             complement(2643..3359)
                     /gene="dapD"
                     /locus_tag="KR314_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBH0326986.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLNEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(3583..4332)
                     /locus_tag="KR314_000583"
     CDS             complement(3583..4332)
                     /locus_tag="KR314_000583"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBH0326987.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDNEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(4399..5286)
                     /locus_tag="KR314_000584"
     CDS             complement(4399..5286)
                     /locus_tag="KR314_000584"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBH0326988.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(5403..6404)
                     /locus_tag="KR314_000585"
     CDS             complement(5403..6404)
                     /locus_tag="KR314_000585"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBH0326989.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(6829..7710)
                     /locus_tag="KR314_000586"
     CDS             complement(6829..7710)
                     /locus_tag="KR314_000586"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBH0326990.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            8033..9226
                     /locus_tag="KR314_000587"
     CDS             8033..9226
                     /locus_tag="KR314_000587"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBH0326991.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            9432..10550
                     /locus_tag="KR314_000588"
     CDS             9432..10550
                     /locus_tag="KR314_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBH0326992.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            10681..10926
                     /locus_tag="KR314_000589"
     CDS             10681..10926
                     /locus_tag="KR314_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBH0326993.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            10913..11659
                     /locus_tag="KR314_000590"
     CDS             10913..11659
                     /locus_tag="KR314_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH0326994.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            11944..12819
                     /gene="hslO"
                     /locus_tag="KR314_000591"
     CDS             11944..12819
                     /gene="hslO"
                     /locus_tag="KR314_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBH0326995.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPDDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            13210..14010
                     /locus_tag="KR314_000592"
     CDS             13210..14010
                     /locus_tag="KR314_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBH0326996.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            14537..16777
                     /locus_tag="KR314_000593"
     CDS             14537..16777
                     /locus_tag="KR314_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0326997.1"
                     /translation="MSNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNDIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSRDGEISEATKKKLSKLLIEDRCPICDGRRLNEGVYRSLI
                     NGYNIADLTSMQIDELAEVIKSINEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHVVDVGPKAGKYGGKVVYEGSYKNLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEIKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQRKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            17191..18726
                     /locus_tag="KR314_000594"
     CDS             17191..18726
                     /locus_tag="KR314_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBH0326998.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTAMTISGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGIGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNISDLKNVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVIVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSEVFKMVGDILTDKEMAKMNYDVENNNLEPREVACEFLNKK
                     GLL"
     gene            18742..19503
                     /locus_tag="KR314_000595"
     CDS             18742..19503
                     /locus_tag="KR314_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0326999.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            19841..20709
                     /locus_tag="KR314_000596"
                     /pseudo
     CDS             19841..20709
                     /locus_tag="KR314_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            20903..21154
                     /locus_tag="KR314_000597"
     CDS             20903..21154
                     /locus_tag="KR314_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBH0327000.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            21266..22045
                     /locus_tag="KR314_000598"
     CDS             21266..22045
                     /locus_tag="KR314_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0327001.1"
                     /translation="MNNTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(22180..22830)
                     /locus_tag="KR314_000599"
     CDS             complement(22180..22830)
                     /locus_tag="KR314_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327002.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIAFPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIDHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(22827..23330)
                     /locus_tag="KR314_000600"
     CDS             complement(22827..23330)
                     /locus_tag="KR314_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0327003.1"
                     /translation="MKLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGRFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(23379..23894)
                     /locus_tag="KR314_000601"
     CDS             complement(23379..23894)
                     /locus_tag="KR314_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH0327004.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKIDVEENHCIDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            24210..24701
                     /locus_tag="KR314_000602"
     CDS             24210..24701
                     /locus_tag="KR314_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0327005.1"
                     /translation="MRTIDIKGENMRELLKWVSIINRYNTNYIDRKLADIGINNSQYF
                     YVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVKDKRTWHLYPTE
                     KSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMNEEAKLEAKENG
                     RKI"
     gene            24685..26088
                     /locus_tag="KR314_000603"
     CDS             24685..26088
                     /locus_tag="KR314_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0327006.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(27030..27623)
                     /locus_tag="KR314_000604"
     CDS             complement(27030..27623)
                     /locus_tag="KR314_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0327007.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
     gene            28471..29121
                     /locus_tag="KR314_000605"
     CDS             28471..29121
                     /locus_tag="KR314_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBH0327008.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRMYFNRKNRIVTK"
     gene            29335..30006
                     /locus_tag="KR314_000606"
     CDS             29335..30006
                     /locus_tag="KR314_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0327009.1"
                     /translation="MAKILVVEDEKRMQSIIVEYMQKGGYTCITANDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            30003..31133
                     /locus_tag="KR314_000607"
     CDS             30003..31133
                     /locus_tag="KR314_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0327010.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDAISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLIINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRIIEQDEISKIWSKFYRSVQSQ
                     RKGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            31538..32383
                     /locus_tag="KR314_000608"
     CDS             31538..32383
                     /locus_tag="KR314_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327011.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            32383..33411
                     /locus_tag="KR314_000609"
     CDS             32383..33411
                     /locus_tag="KR314_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0327012.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGVKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            33414..34427
                     /locus_tag="KR314_000610"
     CDS             33414..34427
                     /locus_tag="KR314_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0327013.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTML
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGAPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILVVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(34909..36159)
                     /gene="cme"
                     /locus_tag="KR314_000611"
     CDS             complement(34909..36159)
                     /gene="cme"
                     /locus_tag="KR314_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBH0327014.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHNIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLVMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIVAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(36245..36991)
                     /locus_tag="KR314_000612"
     CDS             complement(36245..36991)
                     /locus_tag="KR314_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0327015.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(37106..38128)
                     /locus_tag="KR314_000613"
     CDS             complement(37106..38128)
                     /locus_tag="KR314_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBH0327016.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWICKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(38128..40548)
                     /locus_tag="KR314_000614"
     CDS             complement(38128..40548)
                     /locus_tag="KR314_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0327017.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLALLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKILINQGNLNTFGTNLVNDILSKTRHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSDSEIGDEDDYAVIETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(40634..41554)
                     /locus_tag="KR314_000615"
     CDS             complement(40634..41554)
                     /locus_tag="KR314_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0327018.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHFVDIYLPIEPF"
BASE COUNT        15182 a         5228 c         6735 g        14483 t
ORIGIN      
        1 gactataaaa taataaacaa tataattcca tataccaata aataagataa tctttaagaa
       61 aaatgattat atttaaaagg taagcataaa atttataaac ttttagaata gaaatctcaa
      121 tcaattagat attcaaattg atgaatatga atacctaaat agattgagat ttattatgta
      181 caaaacttat cctagtatat ctgacatttt ataaagtcca gattgcttac caattaaaaa
      241 tttagctgca actatagaac cgtttgcaaa tacttcatca gattgagaat gatggcttaa
      301 tgtaagaact tcattatcac ctgcaaatat gacatcatgg tcgctgatta tattgccacc
      361 tcttatagag tgtattccaa tttcatttgg ttttctttta caatctctac catatctacc
      421 atgtgtattt tcactttcag gcaagacttc tctaattgca ttagctatca tagtagcagt
      481 tccactaggg gcgtcttctt ttctattgtg atgtttttct atgatttcaa tatcaaaacc
      541 ttctaacatc tttgaagcat ccttaactaa ttttattaat atattcactc caagagatgt
      601 atttgatgat aataatatag gaatgttttt tgatgcttct tctattttat ttaagtcatt
      661 ttcatcatat ccagtagtag ctaatactac aggtgtatta ttttttagag catatgacaa
      721 tattggctct aaagtctcat gatgtgaaaa gtctattatg acatcagttt tttcagttac
      781 atcagtcatt gttttgtaaa gctttgtatc actatgatga cctttttcat cagcagacct
      841 agctacacca caaaccaact ctaattcatt atcttttttt acacattctg ctaaaactct
      901 tcccattgtt ccattatagc cattaattat aactttcaat ataaattcct cctatgaaat
      961 taataaaaat ttgttatatt tctataagtt tattttatca ctaatattat ttatataaaa
     1021 taatattagt gataataagc ttacaattta tcttacatta aaaaaattta agtggatttt
     1081 tcaaaatata gataaacttc tttttcattg tataaatgat aaaaatatat ttgacttcat
     1141 atcaaaaaga gtgccccatg aactaaaaag ttaattttga gataccatat tcatatatca
     1201 tatgatttat ttggaaatca atattaatac attaaaataa ctaaatacat gtatgcataa
     1261 agatgtataa aaaatatata aacatatagt attaatttta aattcaacaa tttaattgtt
     1321 aaaagtgtta ttattaaatt agaaatatct taacttaata tactgagatg ggagtgatag
     1381 atatgttaaa agaagaaaaa acttatcaag agaaagacgc aaaagtattc aatggagtgg
     1441 cagctttgat aatgattgta ttatttttag tagtttcaat aggtatcatg gtttttggga
     1501 tagtaaaact tgatagtgga tttactggaa taggttcact tatgttaatt ttgggacttg
     1561 tatttatagt agtagatttt atcctatttt ttgggctaaa aatgattaat cctaaagaag
     1621 ctatagttct agttttgttt ggtaactatt atggaactat aaaaaaagaa gggtactatt
     1681 gggtgaatcc attttgttca gcaataaacc cagcagtatc aagaattaat gtatcgaaat
     1741 cttcatctga tgaaaaggta gatatatcaa ctaattcaag gggcaaaaaa gtatctttaa
     1801 aaacaatgac tcttaataat gaaaagcaga aagtcaatga tttattggga aatccaatta
     1861 taataggagt ggttgtaatt tggaaggtta taaatgcaac aaaggcagta tttaatgtgg
     1921 ataattataa tacattctta tctattcagt gtgattctac tattcgtaat gtttcaagac
     1981 tttatccata tgatgtatct gaagatggag atgaaaaatc tcttcgtggt agtagtcagg
     2041 aaatagcaga tagattaaaa gatgaattac aatcaagggt tgacattgca ggaattgaag
     2101 tgtgtgaagt gagaattact catctttctt atgcaccaga gattgcagca gcaatgttac
     2161 aacgtcaaca agcagaggct ataatagcag cacgtaaaaa gattgttgag ggggctgtaa
     2221 gtatggtaga gatggcattg actaatttaa gtgaaaataa tgttgtgact ttggacgaag
     2281 aacgtaaagc agctatggta agtaatctgt tagttgtgct ttgtggcaat aaagatgcac
     2341 aaccagtagt aaacagcagt tctatttatt aaattataaa aagtttaata gtttattaca
     2401 ttgatatatt gaaaaattga aggaaataaa aatacttgtc atcaagaaat aaaaatgata
     2461 taagaatgaa taagagaatt gtaaaaaaat aaaatgatta taaataaaag ataaaaatat
     2521 tataaaaaag agggtgcctc tgatgaatta aattcataca gagacacctt tttaattaat
     2581 tttttaaatt agtatattat tgtttaatta atctttctaa ttaataatat agtactattt
     2641 aattagtcta aatttctaag gtcttccata agcttagttt tgtcagccgt tttttcatct
     2701 ttcattttta taactttagc aggagaacca gcaacaacgg caccagcttc tacatcagta
     2761 gttacaacag caccagctgc aacaacagca cctttaccga ttctaacacc ttcaagaata
     2821 actgcattag ctcctattaa aacatcatct tcaactataa caggagtagc agaaggtggc
     2881 tctaaaacac cagcaacaac agcaccagca cctaagtgaa catttttacc aagagttcct
     2941 ctagctccaa taacagcatt catatctacc atagaacctt caccaataac agctcctata
     3001 tttataacag ctcccatcat tacaacagca tttttttcta tagtaaccat gtctcttatt
     3061 attgcacctg gctcaattct tgcatgttca tttaaagtat tttttagagg aatagcagaa
     3121 tttcttctat catattctac atgagtatct gttatatcat tcccatgttt atctaatact
     3181 tccattatat cctcatagtc acctacaaat atataagaac cattacttcc aaatacctta
     3241 aatttatcag ttgattggaa gtgtaaagag ttagtattta catatacctt aacaggtgtt
     3301 ttctttttaa cttcttttat ataccttgct atttcataag catcgtttaa atttaccatt
     3361 tgttaattct ccttgtattt cattatattt cttttattat atacttattt gtcaatttta
     3421 gtatatactt acttcttatt atttatattt tattttagtt agtttttatt gtatagacat
     3481 agtattaaat aaatttatta tatatgtttg atttgtattg cgtattatta tatctatatt
     3541 gcatatgttt aatttatatt gtgtattatt atatttatac tattatccca acatgctgtc
     3601 catattataa aatcctggtt tttgcttaac taagtatttg gcagcagcta tagaaccact
     3661 tgcaaagata ccacgagatt gagcttgatg gcttattgta agaacctcat tgtctccagc
     3721 aaatagagca tcatgttcgc caacaatatt tccacctctt atagcatgaa taccaacttc
     3781 atttggttgt ctttttgcac tacgaccata tctaccataa ttattttcaa cgtttggcaa
     3841 aacctctttt atagcgttag caatcataac agctgtgcca cttggggcat ctacttttct
     3901 gttgtgatgt ttttctatta tttctatatc aaatccttct agtaatttag ctgcatcttt
     3961 aaccaatttt actaatacat ttacaccaag agacatatta tatgattgga atacaggaat
     4021 aactttagcc gcttcacgta ttttgttcat ttcgttatca ttatatccag tagtagcaat
     4081 tactaaaggt gttttagtct ttaaaacgta agataagata ttatctaaat tagagtgatg
     4141 agaaaaatct ataactacat cagcttcctc aactatagta ctcatatctt cataaatttt
     4201 aaggtcgcct tcatagctat ccagacctct agctgcaaca caaaccaatt ctaattcatt
     4261 atcttctctt atagtatcgg ctaaaatctt acccattttt ccatcataac cagttgctat
     4321 aacttttaac ataatttcct cctatgatta agggctaact tttgaccaaa cttacttttt
     4381 accatgcatt aatctttact atgcattaac ttttagtcca aagttaacta attctttctt
     4441 taacacttct aaatttgctg ggtccatttc tgctagaggt aatcttaaat ctccaacatt
     4501 gaatcccaat aaattcattg cagtcttaac tggtatagga tttacttcta taaataaagc
     4561 tgcgattaga gcatccatac caagttgtaa ttttctagaa ccttcaatat cgccttcaaa
     4621 gaatttagct accaaatcgt gagtttcttg agggcaaaca ttagcaagta ctgatataac
     4681 tccttgacct cctaaagata ataaaggaag tatagtatca tcattaccag agtagatagc
     4741 aaagtcatct ggaactaaac tagctatttc tgcaacttgt cctaggtctc cactagcttc
     4801 ttttacagca actatgttat caattttagc aagttcaact ataaggctag gacttatatt
     4861 tacacaagtt cttccaggga cattgtaaag tataattgga agtttaacac tgttagctat
     4921 tgtttcaaaa tgacgcttaa gtccagcttt attagcttta ttgtaataag gtgttattat
     4981 taatagagca tctactccta atttttcagc ttcttgactt aagttaactg agtgcatagt
     5041 atcattagaa ccacttccag ctataacagg aactctttta ttaacttttt caacaacata
     5101 cttaatagta gcaagttgtt cttcatcagt catagtatta gcttcaccag tagtaccaca
     5161 agatactata gcatcagtac cattagctat ttggtattca actaattcac ctaatttgtc
     5221 aaaatcaact ttgttatcct ttgtaaatgg tgtaactaga gcaacagcag aacctttaaa
     5281 tatcatataa aatcaatccc ctctttttta tttttgtgtt ttatattaca gttacttcta
     5341 atgcttgttt ttgagttact attaatattt tacaaaatta tatatttata caagtttgta
     5401 tattatacta aatcgtatga taaagccatt tcagctattt gaattgcgtt agttgcagca
     5461 ccttttctta tattgtcagc aacaacccaa agatttacac cattatcaac actaaaatct
     5521 cttcttattc ttccaacaaa tacttcatct ctatcatcaa attctacagc agttggatat
     5581 tcgtttttag caggattatc aactactaca acacctggag cttcttcaag tgtttttact
     5641 aaatcatcta actcaaatgg attgttaaac tcaacattta tagattcact atgaccattt
     5701 tttactggaa ctcttacagt agttgcagtt atttttaaat catagttatc aagtattttc
     5761 atagtttcat ttaccatttt catttcttct ttagtgtatc cattttcagt gaatgaatct
     5821 atatgaggaa gacagttgta agagatttga tgagggtaga atccagtagg attaccgttc
     5881 attcctttct ctaaatcttc aacaccttta acaccagaac cagatacagc ttggtaagta
     5941 gagtaaacaa ctctcttaat tttgtattta tcatcaagag ctttaagggg aaccatagct
     6001 tgtatagtag agcagttagg attagctatt atacccttat gattttttat ggcttctgga
     6061 ttaacctcag gaactactaa aggcacatct ggattcattc tccattgact agagttatct
     6121 actactaaga ttccatttga agcagctatt ggagcatact tttcactaat acttccacca
     6181 gcagagaata atgctatttg tatgcctcta tcagtaaatg aagtttcctt taattcttct
     6241 actatatatt ctttaccaca aaacattact ttagaaccag ctgatttaga agaagagaat
     6301 aaaaatagat tttctattgg aaaatttctt tcttctaata cttttaaaaa tgttctacct
     6361 accattccag tagcaccaac tattgctaca tttacttttt tcatataaat tcaccccaat
     6421 tttaaattat taaaacatta ttttatttta gttatacaag taagatatgt taatattttt
     6481 atatatccaa aaactttaat attatattaa caactttaat tatatacaaa aaacgtacaa
     6541 aggtatactt tgtacgtcta atttatcact caaagtaaac cctgtagctc atcacctaca
     6601 aaaataagtg acagtattac acatgttatg tgcaatccca gaaaatatta ttgcaagaaa
     6661 tattttcttc ggctgtaaat cctttttcca atcattaaca cctgccagct actattggat
     6721 actcttagtt acagctacct ctaccctagg ttaataggat attaaaattt gattattcta
     6781 ataataatac atatttaaga aaaaatcaac ttatttatat aaatattgct aaaacttaaa
     6841 tccaaaattt gtcaattctt gttttaacac attaagatta gtttcttcca tttcagctag
     6901 aggaagtctt aaatcaccaa cattaaaacc taaaatgttc atggcagttt taacaggaac
     6961 tggatttact tctataaata aagcagcaat taaagcatcc atatcaagtt gtaatttttt
     7021 tgagccttca atatcacctt caaagaattt agttactaaa tcatgtgttt ctttaggaca
     7081 tatgttagca agtacagaga taacaccact tccacctaat gataatagtg gtagtatagt
     7141 atcatcatta ccggagtata tagcaaagtc tttaggtact aatttagcta tttctgcaac
     7201 ttgagctaag tctccacttg cttccttcac agcaactata ttatctattt tagcaagttc
     7261 ggccacaaca gatggcttta tattcacttt agttcttcct ggtacgttgt ataaaataat
     7321 tggcaactta acgctatttg ctattgtttc aaagtgaagt ttaagacctt tctcattagt
     7381 cttattatag taaggagtta ttattaaaag accatccacc cctaattttt cagcttcttg
     7441 actcatatga acagacaaca tagtattgtt tgaaccagta cctgctataa caggtattct
     7501 cttattaact ttttcaactg tatattttat tacagcaaaa atttcagatt cactcatagt
     7561 agtcgcctca cctgttgttc cacaaacaac tagtgcatct gtgccattct caatgtgata
     7621 ttcgattaat tcacctaatt tttcaaagtt gacattatta tcctctgtaa atggggtaac
     7681 taaagcaaca gcagaacctt taaataacat ataatcaaat ctcctttttt atctaaattt
     7741 aattttttaa gtacaataat aattatagta caaattaatt aattttaata ggtatagtta
     7801 atatcttaac tgtttaaata agatatttat attatattaa tattagaaaa attttaaata
     7861 aatgttttat tctcaaaata tgcatattaa tttaataaaa tataccaaaa tggaaataaa
     7921 aaataaattt tatgagaatt taataactaa taattaatat atttaggtta taataaatat
     7981 gtgcgtaaaa tctaacaata agcaataata ccgtaaattt aggaggaata atatggatac
     8041 tttaaaggaa ctttttaaaa taggaagtgg accttcaagt tctcatacta tgggaccaca
     8101 aagagcagca gagagattta agaatgaaaa cccagatgca gaaagtttta gagcaatatt
     8161 atatggtagt ttggcagcta cagggaaagg tcacttaact gactatataa ttgaaaaaac
     8221 tatagcacct aaaaaagtcg aaattgtttg ggaagaagat attataaaag attttcaccc
     8281 aaatgggatg aagtttgaag ctttagataa agataaaaat gtaacagctg aatggactgt
     8341 atattctgta ggtggaggga ctatagcaga agaaggtcaa agaaacagta aaagcaatag
     8401 tatatatcac ttagatacta tggatgagat agtaaaatgg tgcaaagaaa ataataagac
     8461 tttggtagat tttgtgttgg aatgtgaacc aaaagacata aaagattata taaaaactat
     8521 aaaagatgct atgagaaaat ctattgatga tggactaagt acagatgaaa taataccagg
     8581 caagttatta ttaaaaagaa gagcaagtac attttataat gcatataaaa aagataagag
     8641 cttctcaaca cttgtatatg catatgcact agcagcttca gagcaaaacg cttctggtaa
     8701 tataatagtt acagcaccaa cttgtggttc tgctggagta ataccaggaa tattctttgc
     8761 aatgcaggat ttctataatt atgatgacga aaaaataatt gaagcactac ttgtagctgg
     8821 aataataggt aatataataa aaactaatgc ttcaatatct ggagcagagg tagggtgtca
     8881 aggtgaagtt ggagcagctt gttctatggc agcagcagca gtagcatact taaaaggtgg
     8941 aactatagac catattgaat atgcggcaga aatagcactt gaacatcatt taggaatgac
     9001 ttgtgaccct gtatatggat atgttcaaat tccatgtata gaaagaaatg caatggcagc
     9061 tcaaagagct tatgatgcag ctaattatgc attattaact gatggctcac attctgtctc
     9121 cttagaccaa gttgttgaaa caatgaaaga aactggtata gatatgatgg ataagtacaa
     9181 agaaacagct aagggtggac ttgctaaaca tttcttctct tgctaaaaat aaaattttcc
     9241 aataataatt cctctgtttt gatttatgaa acaggggaat tattattaat ggtgaattat
     9301 ttaataatgc aaaatatttt tatattcaat ttagcttttt atttttaatt agtataacat
     9361 gctatataat attttaatta taaagaaata tagaaatatt ttttaataaa ttagaaattg
     9421 gaggagatta catgagagag gcatcatatt tacaagaaca actttataaa cataggaagt
     9481 cattaaatcc tattgcagag attgggagaa aagagtataa gacttcaaaa tatataagag
     9541 attatcttga taaattagga attgagtatg aagtttattt agaaacaggt atagtaggtg
     9601 taatagaagg taaaagacct aaaaaagata ttgcttttag agctgacata gatggtcttc
     9661 atagtgaaga aggggtaaaa catttatgtg gtcatgatgg acatgctact atattacttg
     9721 gattaattga gtatttaaat gataataaag aagacttaaa tgataatata gtatttatat
     9781 tccaaccagc agaagaaggt cctgggggag caaaagcttt aatagatgaa ggagtgctta
     9841 gaaaatacaa tattgatgaa atatatgggc ttcatatata tccagagtta ccagaaggtt
     9901 atgtaggtac aagagaaggc tattttatgg ctcaaatagg agatatcgac atagaaatag
     9961 tgtctaaaag tggacatggg gctatgccac aaaatggtat tgatggtata gtgatagctt
    10021 caaactttgt aagctcactt caaactatag tgtctagaaa tataagccca atagacaatg
    10081 cagtgttgac tattggtaga attgaagggg gagctagaag aaatataata gctgaaaata
    10141 taaagatgga aggtactatg agatgtttca gcccagaagt gtatgagaaa atgaagctta
    10201 gagttagaga acttgctaga gggtttgaat tggcttataa ctgtaaggtt aatataaata
    10261 ttatagatga ttatattgct gttaaaaatg acaaggattt gtatcaagag tttgtagagg
    10321 ctattggaag tgatactgtt gtagagttag agccacttat gatatcagaa gatttttctt
    10381 attatcaaaa agaagttcca ggattatttt ttatgttggg ttcaagaaac gaagaaaaag
    10441 gatttgtaaa tggtcttcat aatataaact ttaattttga tgaaaagata tgtgtaaatg
    10501 cattgaatgt ttattcaaaa ttattaaaat acaaagaagc aatagattag gaagataaat
    10561 tataaagatt aaaaagcata aatacctaaa tttaaaaaaa atgtattaat aaatacccct
    10621 tatatatttg tataaattta ttttaattga caaaaataca aataagaagg ggtgattttt
    10681 ttgaaagaaa aagtacaaag agaaaagaag ataactaatg gcaagaatac taaaatattg
    10741 acaaatgatg atataatgaa atatgagata gcctcagaac taggtcttat ggataaagtt
    10801 ggtgaacgtg gatgggcagg acttacagct aaggaagcag gaagaattgg aggtatgctt
    10861 acatcacgca aaaagaggct taaaaagatt gtggaggagc agaataaaaa ggatgagtca
    10921 atatagtgat tttgcgtttg tatatgatga acttatgaat gaagttgatt ataatggttg
    10981 ggttaaatat atagaagata taatagatag tgaaggtgta aaagtaaaaa atatattgga
    11041 attggcatgt ggcacgggta atcttacaat tccattgact aaaaaaaatt acgatatagc
    11101 tggtatagat atatctgatg agatgcttag tgtagcaaga gaaaaggcag aaaaagaagg
    11161 agtagaatta gttttacttc aacaagatat atcagagctt gattttgaaa tatctgattt
    11221 ggactgtgtg ctttgtgctt gtgatggatt taattatata acttatgatg atgatttgga
    11281 aaatgtattt tcaaagacat atgaattatt aaagaaagat ggaatattca tatttgatat
    11341 aagttctttt tataaattag ccaacatatt gggaaataac atgtatggag aaaatagaga
    11401 agatatagcg tatatgtggc aaaattactt tgatgaagaa gagaatctaa ttgaaatgga
    11461 acttacattc tttattaaag atgaatgtgg aaagtttgaa agatttgaag agatacacca
    11521 acaaagagca tatactgaag atgagatagt tgatatgctt gaaaaatctg gatttaagga
    11581 tataaaatat tatggggatt ttacatttga caaacccagt gaagaaagtc aaagaatctt
    11641 ctttgtatgt aaaaaataag atatttttga ctatataaga taattattaa aaataaccat
    11701 gatataaaaa tatagaaaat gcagttttat agattaaaag taaagtgatg atactttatg
    11761 acttttattt tctaataatt ataaagttct atagataaaa attaaaatat aataactatt
    11821 gcttagtaaa attaaaataa aatgggtttt gcccaaggtt aatatagata agtacttata
    11881 gattaaaatt aaggtagtaa tataataaaa ataagacaat taacgggaca tggaggaata
    11941 ttaatgagag attatgtact aagagcgaca tcaggaaatg gtcaagttag agcatttgtc
    12001 gcaactacta gaaatacagt agaagaagct agaagattac atgaaactac aaaagtagca
    12061 acagcagcac ttggaagaac attaacagcc acatctataa tgggattaat gatgaaaaat
    12121 gatagtgata aattgactgt aataataaaa ggtggaggtc caattggaac tattatcgcc
    12181 acatcagatt ctaagggaat ggtaaaagga tatgtaggaa atccacaagt agaggttgaa
    12241 gactatccaa atggaaagtt aaatgtagca gcagcagtag gtacagaagg tgttgtaaaa
    12301 gttataaaag acttaggtct tagagaacca tataatggga cttatccatt ggtaagtgga
    12361 gaaattgcag aagactttac ttattatttt gctgtatcag agcagactcc atcagtagta
    12421 gctttaggtg ttcttacaaa agaagatgaa gtagagtttg ctggaggatt tatagttcaa
    12481 ttaatgccag atgcagaaga agagactatt gctaagttag aggaaaatgt agctaaatta
    12541 ccttctataa ctaacatgtt aaaagaagga aaatcaccag atgatatatt aaatattgtt
    12601 ttggatggtt tggaacctaa aatacttgat acctgtgaag tagggtttat gtgtgaatgt
    12661 tcaaaagaaa gagtaaaaac tgctttggtt gcaattggta aaaaatcatt ggctcagata
    12721 atagaagaag ataaaaaagc agaagttggt tgccagttct gtaataagaa gtatatgtat
    12781 agtgaagaag agttgcttga aattttaaag gaaatgtagg ctataaaaat agttgtattg
    12841 atataagtaa tgttatagtg atatagtaaa gtcgtattga tatagtaatt aaagtataat
    12901 ttattaataa tagacatatt tatattaaaa aataaattta ataaattttt aagcttaata
    12961 aattttaaga attaagaagc tttctggctg taatatttgc aatgttgcag ttagaaggct
    13021 tttttatttt attatagttc gtgtcaaaga ttgtatgaaa caataaaata ttgtaaaata
    13081 atttgtatat ttagacctga ttgacacaaa ggtttataag atatttgagt tgattatacg
    13141 aaattctatg aagtagtaac cctgtgactt taatcatggg aggttcagaa gaaggagaaa
    13201 attttaaata tggaaagtaa aacgaagtac ataggaataa gtatattaat attattgatg
    13261 atatttttaa ttgcaaagaa ttttaacgga aaacttgaaa tacctattcc tgagagtaat
    13321 agtagaaaat ttaaatcagc agcagtgttt tatcctcaac atcaagatga tgaggtttta
    13381 tggggaggaa gtgccattgt agatgctata aaacagtgtg gtgttgataa tgtatatgtg
    13441 gtattagtat cagatggttc tggagttaac gttttcaaag ctaacacaaa atttagaaac
    13501 ttaactagga agcaaaagga agagcttaga aataatgagt ttaaatcagc tttgagagag
    13561 cttggagtga aacctcaaaa tgtgattata cttgcagata tagataaaaa ggaaggcaca
    13621 cattatgaat taatggaaaa gactatattg gattttgaac ataagtttaa aggcaatgtg
    13681 acccatatag cccatcacta taagtatgat gaccatataa tgcatagaaa gaatggggaa
    13741 gttttaaaga aacttaaacg tgaaggtaag ataaaagatg acttgtattt tgttaagcca
    13801 aaatatgtaa agtatatacc aagaaatgaa agagctatct ataaagcaat ggattttgat
    13861 gactacaata aggttaagag tgcttgttat gaatataagg ttgtaaattc aaagcgtaaa
    13921 atgtatggta taggatatat ttcggcacat agttattttg aacatcttct aaatgaccct
    13981 gaacttacgt ctgtattaag tgaagattaa tttcttataa gtagtaagtt atgtttgata
    14041 agttgattgg aattttctat agttttgaag gtattggaaa tttagtttta tttaaagatt
    14101 taaagataga ggtttttaat gtttaaaatt ttaaagttaa gttgaataat tgtatagaat
    14161 tatttgtgaa agaagaaaca gtgtaaaaca tagtatctta tttatggttt taaaatcata
    14221 ggcaagatac tttttttatg taactaatac cattttgagg ttattaactt aagaatttta
    14281 tataatttaa cactcatata taatgatgat atttaggtag aatagatgca attacgctta
    14341 aataaaataa ctgtattttt tataaaaatc aaaccctaca ctatactacc agataaaatc
    14401 tacatgctat aatttgacct gtgtttataa aaaaaggagt aatctaataa aatagtattt
    14461 cacttatgta taactaacaa ctattattac gattttctct aaataattta aagaaaaata
    14521 atgcaggagg taaaatatga gcaatgaatt tataatttta aaaggttgca aagagaataa
    14581 tttgaacaat atttccttaa aaataccaaa aagaaaaata accatattca caggggtgtc
    14641 gggttctgga aagtcatcta ttgtatttga gacaattgca aaagaatcac aacgccaact
    14701 aaatgaaagg tttagtacat ttgtaagaag ttttttacct aagtatggag aggttaaagc
    14761 tgattgtata gaaaatctgt caacaccaat tatcatagac caaagtagac ttggtggaaa
    14821 ttcaaggtca accctaggta ctattacaga tgtaaattcc tttttaagag ctttatattc
    14881 aaggtttggt agtgagtata taggtaatgc taatatgttt tcttttaatg atattgatgg
    14941 tatgtgccca cactgtcatg ggcttggaaa aaagctagtt cctaatatga accaaattct
    15001 tgatatgaat aaatcattaa atgaaggtgc tatattgctt tcagggtttg gagtgggttc
    15061 ttggcattgg aatatattcg ctgaatcagg attttttgat attaataaga ggatatgtga
    15121 ttattcagaa gtagaattgg aaaagttttt gcatggtaaa gcagaaaaga ttaagataga
    15181 aaatgctggt caaactaaca tgacatatga gggattaatg gttaaattta atcgtctata
    15241 cttaagtaga gatggagaaa tatctgaggc tactaagaaa aaattgagta aacttcttat
    15301 agaagataga tgtcctatat gtgatggaag aagacttaat gaaggagtat ataggtcttt
    15361 aattaatggt tataatatag cagatttaac tagtatgcaa attgatgaat tggctgaagt
    15421 tattaaaagt ataaatgagc cagaagcaca acctttaatt aaaggaataa tagaaaagtt
    15481 aaacagtatc atagatatag gtcttggata tttaacactg gatagagaga catcttctct
    15541 atcaggaggg gaatctcagc gtataaagat ggttaagtat ttaaatagta atcttgttga
    15601 tttaatgtat atttttgatg aaccaagtgt aggacttcat ccaaaagatg tgtataaatt
    15661 aaataatctt ttaaaaaaat tgcgtgataa agggaataca attatagttg ttgaacatga
    15721 cccagatgtt attaagattg cagaccatgt tgtagatgta ggaccaaagg caggtaagta
    15781 tggtggaaaa gttgtgtatg aaggcagtta taaaaatcta cttacaagtg gtactttgac
    15841 agggaatgct ttaagtaaat ttcttcctat taaagaaagt gtaagagaac atagtggata
    15901 tttagaaatt aaaaattgta ataaaaacaa tttaaaaaat gtatctatta agatacctaa
    15961 aggtgtattg actctagtta ctggagtagc aggagcaggt aaaagtacgt tgattaagga
    16021 tgaattttta aagcaaaatc ctagtgctgt acttatagac caaagccctg tttcagcaaa
    16081 ttcaaggtcg agtttggcaa catatagtgg aattatgaac aacataagaa gcatattttc
    16141 aaaaacaaat ggcgtaaatg cttctttatt cagttcaaat tcagaaggag cttgtgataa
    16201 ctgtaagggt tctggaatag tagaaatgaa tcttgctttt atggagagta taaagagtac
    16261 ctgcaatgtt tgtgaaggta aaaaatttaa aaaagaagtt ttagaatata ggtttcaaag
    16321 gaagaatata attgaagttc ttgaaatgtc tgttttagac gcaattgagt tttttaattt
    16381 aaaacagatt aaaacgaaat tacaatgtat tgaaaaaatg ggtattggat accttacttt
    16441 aggacaaact cttgatactt tgtctggagg agagtgtcaa agattaaagc ttgctagtga
    16501 attaaataag gaaagttcag tgtatatatt agatgaacca acaacaggtc ttcatatggc
    16561 tgatattgag aattttataa gtataattga agagattgta gataatggaa atacggtcat
    16621 aataattgaa cacaatatag atgtaattaa aagagctgat tggattattg agctgggacc
    16681 tgaaggaggg acaaaaggcg ggagagtaat atttgaaggt atcccaaaac aattatgtaa
    16741 caataaaata tcattaacag caaaatatat agtataataa ataaaatata ataaaattat
    16801 aatgatattt taataaatat aatgggtata taaaatgata attaaactta acagtaataa
    16861 ataatgtgag aaatattcaa atttaattaa ataaagtgtc agaaatttgt ttaaatttta
    16921 gtattttaac gattctgacc ttttttctta tttttatgga gagtatcgaa gatggagtct
    16981 ctaatagtta cttttttgtg tctacttgca agtttgatta aaaaatggct atacacactt
    17041 gtgacttcag tcatgggagg ttcagattat aatcttaata tataatttgt gaaataattt
    17101 gatgacgatt tgctgatgta taaacaataa agattaacta cattaaaatt taaatcttgt
    17161 taagtaaaac ttataaaagg agagagatgg atgataaatg atattctaac cttattatct
    17221 gaaagaagtg atttttttat aaagctatcc atagaacatt tgatgatttc tattatttct
    17281 ataataattg cgattgtttt gggtggtata attggtatta ttgtaagtga atttcataat
    17341 tcttctaaac ctatattggg aactatcaat tttctataca ccattccatc tatttctatg
    17401 ctaggatttc tgataccatt ttccggaatt ggaaatacta ctgctgttat tgcacttacc
    17461 atttatgcat tattgccaat ggttagaaat acatatactg gaattactaa tgtggataaa
    17521 aatattcttg aagcagctag gggaatgggc agtacagatt ttcagatatt gtataaaata
    17581 aaacttccat tagccctacc tgttattatg tctggtatta gaaatatggt gattatgaca
    17641 attgcattag ctggtatcgc ttcatttata ggtgctggag gcctaggtgt agccatatat
    17701 agaggaatca cgacaaataa cacagcaatg acaattagtg gaagtatact tattgctcta
    17761 ttagctttaa tatttgattt tattttaggg ctggtagaaa aattttttca acgaaaaaga
    17821 aaaaagaaaa ataggaaaaa agtttttgcc attctaggaa gccttattct tatagcaact
    17881 atctttacgt cttatttatt taatccttca aagactcaaa ctattcatat tgctactaaa
    17941 cctatgactg aacaatatat tcttggtgag atgttggata ttttaattga aaaaaacaca
    18001 ggtctaaacg tggaaattac acaaggcata ggagggggaa catctaatat ccagccagct
    18061 atggagaatg gagaatttga catttatcca gagtatacag gtacaggctg gaatatggta
    18121 cttaagaaaa atgaattata tactgaaagt atgtttagta aactacagtc ggaatacaaa
    18181 actaaactta aaatgcaatg gactggtatg tatggttttt ctaatagcta tgcattagca
    18241 gttagaaaag aagttgcaga taaatataat ttaaaaaata tatcagactt aaaaaatgta
    18301 tcttctaaat tgacatttgg agctgaatat gacttttatg agagagagga tggatatact
    18361 ccattgtgta atacatatgg actgaacttt aaagatacag ttgatttaga tattggatta
    18421 aaataccaag ctatcaatga aggtaagatt gacgtaatgc ctatatttac aactgatggt
    18481 cagtatagtg tttcagatgt tatagttcta aaagatgata aaaatttcta cccatcttat
    18541 caatgtggaa atgttgttcg tttggaagta ttagaggaaa atccagaact atcagaggta
    18601 tttaagatgg ttggagatat tttgactgat aaagaaatgg ctaaaatgaa ttatgacgta
    18661 gaaaacaaca atttagaacc tagagaagtt gcttgtgagt ttctgaataa gaaaggtctt
    18721 ttataaattg gaggaaatat aatgacacct attatacaat ttaaaaatat taaaaaacaa
    18781 tataatgata aaactataat agataatctt aatttagata tagaaaaagg agagtttttg
    18841 actgtaattg gttcgtctgg ttctggtaag acaacactct taaaaatgat taatggtctg
    18901 attttgccag atggaggaaa tattttgata aaccaaacag atatcaaaaa cgaggactta
    18961 attaaattaa gaagaagaat aggatattgt gtacaaggaa gtgttctttt tcctcatatg
    19021 actgttgaag aaaatatttc atatgtacca aatctcctaa gtaaaaagaa taaattagaa
    19081 gtaaaaagtg ctgtaaacaa gtggatggaa atagtgggat tgccaaatga tatgaaagtg
    19141 cgttacccat cagaattgtc aggtggtcag caacaacgtg tagggattgc aagagcttta
    19201 gcttcttctc cagaaatttt attaatggat gaaccttttg gtgcagttga tgagattaca
    19261 cgaaagcaat tacaaaaaga gataaaagaa attcataaaa aaacaggagt tacgattatc
    19321 tttattacgc atgatattta tgaagctttg attttgggaa caaagacttt ggtattaaat
    19381 catggagttg ttcaacagta tgatactcct gagaatattt taaatactcc tgctaaccaa
    19441 tttgtagacc agttattgaa tataagaaaa tctataatag atgatgataa tttaaaagaa
    19501 taaatttaaa tttaaaatca aattgttgtg ataaaaacta ttgatttaga gctaacttca
    19561 agttgtatac ttgaagtgga aaagttagat taaggtatag aagagaattt agcagttggc
    19621 tgtagacgag gtaactgctg aaggtgcttt caataaatgg ctagaacctg tatctattga
    19681 agattataat aaattgaaat aagaagctca atatacagag tagataggca cttcaaatag
    19741 aagtgtcttt tgtgtgccat aaaataaatc aaaatattag caaagaatgt gaaaaacaaa
    19801 ataaaataat atgctatctt taaaatagga ggggtgctat atggataaca atttgattac
    19861 cattgaaaaa actttagatt atatagaaaa acatctttta gatgagatgt tagatttaaa
    19921 tgtaatttct aaagagatag gctattcaaa gtaccattta catagaatgt ttacatcagt
    19981 tgtcggattt agtatacata attatataca aagacgtaga ctaactgaag cagcaagaat
    20041 gctcattttt acagaaaaac caattattga aattgcttta atctcggggt atgacacaca
    20101 acgttctttt tcaacagggt ttaaaaaaat gtttaagcat agccctaagt tttttagaaa
    20161 acatcaagat tttttaccac tacaattaaa atttgatatt aataatagaa aaaggttaca
    20221 tggaggtatg gttttagatg ttaaaaaagt taattgtaca gatataaata ttgttggcta
    20281 ttctggtaaa gtaaaaaaag gatttcatgt tattggaaaa tgttggaggg atttgcataa
    20341 aaataaagag ttgatagaaa acaaggtaga cttggatttt ttaattggag ttaatgatta
    20401 tagtaatttt aaagaaattg aaaataatcc tacatttaca tatattgcag gtgttcaagt
    20461 agctactttt ggtaaattcc taatggaatg gaaaaattcg tattaccgaa aagtaattat
    20521 attgttttta cttttattgg gaaaaatgag gatagtatgc aaaatgttgt agaatatatc
    20581 tatcaagaat ggttccttaa ttcaagttgc cagtttaatg aaaataattt atatgatttt
    20641 gtaaaatacg gagaaaaaac tgataagaat ggtttgagtg aaattcagtt ttgggttcca
    20701 atcatttaaa agaagggatt atgtataaag taggtggatt tatataggca aagttttata
    20761 aaagtatgta tttataaata atttatagtc tgtaggaaag aaatagtttt actataagat
    20821 tatgataaaa tacacaatac tatgataaaa tagtactaaa taatagattt aattttgcaa
    20881 aggataaagt gttttaataa aaatgaatgt agaaaacgaa ttacttaaaa acctagatag
    20941 attgcataca acagagttag gcgttgtaag aattaagaaa aatctttctt tggagacaaa
    21001 tgatgtagtg aactggtgta aaactaaaat agaatctcca aatgccatta ttaatagaaa
    21061 gggaaaaaac tggtatataa gtgtatatga ttgtattata acagtaaacg ctcacagtta
    21121 tacaattatt acagctcata aagaaaagaa atgaattgaa aaatacaaag gtcaaaagca
    21181 ttttccactg tttgtagtac ctatgaagaa gtatataaaa taatattact tttgaataat
    21241 aatttaaatt ttggaggttg aaaaaatgaa taatactgta aaagaaaaat tggcagagtc
    21301 actaacttct gatactactg atattttacc ttatttgcct tatttattac aagatttttg
    21361 ggaattagga agttcaccag aacaaatgtt acaattattt agaaagcata taccttttaa
    21421 tgaggataca aaagttttgg atttagcttg tggaaaaggt gctgtatctg tgaagcttgc
    21481 aaaagagttg aatatacata tcaaaggtat tgatttgata cctgaattta ttgattatgc
    21541 gagaagaaag gctattgaat ataaggttga tgctttgtgt gattttgaaa tgaatgatgc
    21601 taatagttct gtaaaaattg agcacgatta tgattgtgtg atttttggag ctgtagggga
    21661 tattttaggt aattataaag aaactataag taagctatta cctacaataa aaagtaaggg
    21721 gttcatttta attgatgatg catatgtagt agataaatct acaaatgaac agcttaattt
    21781 tgagagggtg tatcccactt acaatgagtg gtgtatattg ttcaaagaat taggtctgaa
    21841 actaattgaa tgtataccat gtttagaact tgaaaatgat gttgaagagg aaatgaactg
    21901 gattataact cgtgcaaatg agctaattaa aaaatatcct tctcaaaaag atatgtttga
    21961 acaatatgtt attagccaaa aaaatgaata tactgattta cagcaggacc ttgttggagc
    22021 aacttggatt ttacaaaaag aataagataa atattagact agataatcaa tgttgtagtg
    22081 aattatccca tgtctagtga atataaataa cataaaaaac cgtcaagatg aatagtttat
    22141 tcgtgcttga cggtttttag tatttgttga agtaaaattt tatttcttta gtatatcact
    22201 tttagtcaat ccatttgatt tagcttgagt attccattga tgattcccca taagtttata
    22261 taggaatcta gggaagttag gtgaaataaa aatagtactt ttgttggaat catctatatg
    22321 tatattatca ctaagtatat tttttgaaag ttcttttaat gcaattccta aatctttttt
    22381 aggtccttta cccaaaggta cggattctag tgaaccaaac atctccccag ctccaattcc
    22441 aataccttga acccatgtaa agttgatttt tttgcaccaa tgatctatca tttgaattgc
    22501 caaatgatta tgttcacctt cattgaatcc acagttaact attgcataca ctggaatagg
    22561 ttttttattt tgtgttttta aaaatttttc acatttctct aaaaaatgaa gtaaatgaga
    22621 tggaatacca tctacataaa gaggaaatgc tattattaat ctatcacaat taagtatggc
    22681 attaagagcg tcaggatact tagatgtgct atctacttta aatgtaaaaa ctttattatc
    22741 attttgaata agttgtttta attcagataa aaaatactgt gaattatttt tatttacttt
    22801 tggactacca tttatcatta ctatattcat atggattcac ctcttatatc ctgtatattt
    22861 tcatagaaac aaacagagtg ttcttttata ttgaaattta tagcatttcc atttactaat
    22921 ttcgtagcag tttctttttc agcttctgta atacaatctc catagaatat agcttttaaa
    22981 tttatctgtt tatcatatct gcttttatga cgcatcatat tatcttttat tacaaagtat
    23041 ggaagaatat aattaatgct tttatcaagt acgtttttga caaaagggct aaaccctccg
    23101 taacagcatc tgcttattat aataattcca tcggattcat ttataagttt acccatgttg
    23161 ctgtaataat cttttacaac acattcagaa ggagttttaa tccagcaacc aaaacgacca
    23221 atacatttgt ggatttcttt tgtgtttgga agtattttta tattgtcatc aagcttgggt
    23281 aataactttt ctaattcttt gtcagttaaa tcatgtatta aaagcttcat aaagtccatc
    23341 tccttttatt atgtatgttt atttctaaaa attatatctt atagattttt cagtaaatct
    23401 ttacaccaat taataacaaa attataatag tcaactccat aaatcattgt ggcatacata
    23461 tatgtatatt ggtcaataca atgattttct tctacatcaa ttttttgttt acttaattta
    23521 tttagaagta attctacttc agttattaac atttttaagt ttgtttcaat tatttctttg
    23581 ggtagatact tgtaaaagaa tatattaaca aggtggtctt gctttgtttt agaaaaattt
    23641 ataggtttct ttaaccattc gagaaatact tctttaccag tattagttat ggaatatagt
    23701 ttttttaatt tgctaccatc tataacttcg tgatagtgta tatatccttt tgtttctaat
    23761 cttttaagtg caggatagat acttccaaag ctggcgtcaa aaaagtacga ggtactttct
    23821 gacattattt gttttaagtc gtatccactt aattcttttt ccattaaaaa tcctaaaata
    23881 atgtattcta acatattacc tcctggtatc aatcagatat atatcgattc gatatattta
    23941 ttattacaga taataaatat tacgtcaaga tgctttttaa aataagctaa aaagaattag
    24001 gaattattga taaatatgca aaaaaattaa tataaaaggt aattaaatat caatgaagat
    24061 ttaaaaatag ttaaattaaa gtaagagtct aatatataaa atttatagta actttatggt
    24121 ttaaatataa acttgtgtgc tattgacaaa gtataaaatt agcagtaaaa taattgcatg
    24181 agcaaataga ttattgcgaa cgcaaaaaaa tgaggacaat agatattaaa ggagagaata
    24241 tgagagagct attaaaatgg gtttccatta taaatagata caatacaaat tatattgaca
    24301 ggaaattggc ggatattgga atcaataata gccaatattt ttatgtatta cacatatgta
    24361 ataatccagg tattacacag gacactatat ttgagaaaat tcttgtgaat cctagtaata
    24421 taacgagagc tttggcaaat ttagaaaaag aaggctttat aacaagagaa ccaggtgtaa
    24481 aggataaaag aacatggcat ttatatccta cagaaaaatc tttagaatgt tatgatgaaa
    24541 tactaaaaat aacaaatggg tatgttgagg agttgttgag ttcatttgag aaagaagagc
    24601 aagagttgtt tatgtcaatg ttaaagaaag ttgcattttt agctattgat atgaatgaag
    24661 aagcaaaatt ggaggcgaag gaaaatggaa gaaaaatata aggagccatt ggaatatgaa
    24721 aaggtttcaa agttattaac taagtacgca attcctagta ttatagcaat gttagtaagt
    24781 gctttatata acattgttga ccaaatattt attgggcaag gtgttggtgt attgggtaat
    24841 gctgctacaa atgtcacttt tccactaaca acaatatgta ctgcaattgc attattatta
    24901 ggaattggag gagcgtctaa gtgttcattg gaattaggtg ctaaaaattc agaaaaagct
    24961 acaaaagcag caggaaattc tatatgcttg atggctgttt ttggaataag tttatttata
    25021 gtagtatcaa tatttttaac accactatta aaattctttg gctctacatc agaaatacta
    25081 ccttatgcac acacatatac aaaaattaca tcaattggat taccatttct tataatgtcc
    25141 actggaatga gtaaattgat acttgcagat gggagaccaa aagcttctat gttttgtatg
    25201 ttaataggag cagttattaa tacaattttg aatccattat tcatttttgt ttttaatatg
    25261 ggaattgctg ggtctgcttt agcaactgtt attgggcaac ttatatcttt tggaataagt
    25321 gttcattata taaagaattt tcaacatatt aaattacata agaaaagttt taaattagat
    25381 agtataattt ataaacatat atttgcgcta ggttcaagct catgttttaa tcaattagca
    25441 atgactgtaa cacagatagt tatgaataat acattggcat attatggggc taggtcagtt
    25501 tatggaagtg agattccact tgcttgtgtc ggtatcatta ttaaagtaaa tatgattttt
    25561 atatctataa ttattggtat atcacaaggt atgcaaccaa ttatagggta taattatggg
    25621 gctgaaaagt ataatcgtgt aaaagaagca tataagtttg cagtgggaag tgcaactatt
    25681 atatctgtta ttgcattctt atgtttccaa ttcttcccaa gacaaattac gtctatattt
    25741 ggtaacggaa atgagactta ttttatattt gcagaacatt attttagaac tttcttgttc
    25801 ttaacattta taaatggtgt tcaaatagta tcatcaactt tctttacatc aattgggaaa
    25861 tcagtgttgg gactttttac ttctttgact cgtcaaattt tatttttaat tccattgata
    25921 gtaattttac caatgtttat gggaattgat ggagtaatgt ttgcaggacc aatagcagat
    25981 gcagcagcgg caatggtatg tatatacttt gcagttcatg aaatgaaatc cttgactata
    26041 aagcaaaata atttgatgag taaccataat aagattgcag aggtataaga caatatttaa
    26101 gaagtataga aaattaattt aagaaatata ataaactaat ttaggaagtg tagaaaatta
    26161 atttaaagaa gtataataaa ctaatttagg aagtgtagaa aattaattta aagaagtata
    26221 ataaactaat ttaggaagta tagaaaatta atctaaagaa gtgtaataaa ctaatttagg
    26281 aagtatagaa aattaattta aagaagtata atgaactaat ttaggaagta tagaaaatta
    26341 atttaaagaa gtatagaaaa ttaatctaaa gaagtgtaat aaactaattt aggaagtata
    26401 gaaagctaat ctaaagaagt ataataaact aatttaggaa gtatagaaaa ttaatctaaa
    26461 gaagtataat aaactaattt aagaaatata gaaaattaat ttaaagaaat acaatacatt
    26521 aatttttaag taacattaaa ttaatgtaag taaaataatg ttcatatgaa gatttaaagg
    26581 tgcattttct gaagctaagt atatgttatg tctctatatc cggtgaagtt taatagctca
    26641 agaaaatgca ctatttatga tgattatttt gttttatttc caattttgga aataactatt
    26701 ctaattaaat ctaaaattat accataaata agaaaagaaa gtatagatgc tatgtaccaa
    26761 taaatcgaaa ttgaccctgt aattgttcta catgggattt gcatgcctga atccataaaa
    26821 acaaaaaaac tatcaatggt agaaataata gacaaaatag gatttaatcc tattagaata
    26881 atactttttg agtcttgacc aatttggtgg atataaatct caaataatgc aattaaaaaa
    26941 aacaaaatgt aaaagattta ataatttttt tcatcaaaat acctcaccta atataaattc
    27001 tgtttgcact atttgcttac tataactatt tatctccatt cagtcacatc acttaattgt
    27061 aatctaggtc ttcttgatgg ttcttcgtca ggataaccta aagatattgc tgccactaat
    27121 tgactatctg tatttaacca ttcacataat tcacgatatg caaagtatac atcacaaatc
    27181 caaagactac ctagacctaa ttccacagct gtaagcgaca tattctgaat tgcagctcca
    27241 attgattgca tgtttgccat ttcatagaat cgttcttcag gggatagctt ttctaaaggt
    27301 gatttaccta atatatttaa aataaaaatt gttactggag cttgtttcat gatttcaact
    27361 gtgtaatttg caccagcgat atgttgacga cttcctggaa gaagcccatt atcattaatt
    27421 tcattttgaa tacctttgct catggcttta agcatagatt ctttttcctt ttcagtgatg
    27481 acaacaaaac gccatggttg cctgttttta gatgaaggtg catttattcc agcttcaatt
    27541 attttctcta tagtttcatg tgaaatgctt tgatttttat attttctgat acttcgtctt
    27601 tttgatatag aatcacttat cattttaagt aacctccgtt tattttttgg aaaaatctat
    27661 ttattttcta tttttaatta ttttagcatt aatcagaaaa aaagtcattg attttagtat
    27721 acaattatta acgattatat taaggattgt atcaaaaagt atgtgattat actaagggat
    27781 tatattagac attatattta aaattgtatg aaaggttgta tttaatatta tattaagaga
    27841 gatatatatt aggatttata tatattaaga tttattatat tgagccctta tattaaatta
    27901 aggattataa tttaaaaata agctataaaa acccaatatt gttttgttca gagataaaaa
    27961 agaatacttt aggtgatttt tattaaaaat taagagtgat aagattatat actttataaa
    28021 tgtaaatatg acgaataaaa atgataaata taataatcta tatatttaca ttaggagttt
    28081 tctatactat aattatttat atttaaaact aaatataaat gttggaatta tttaggattt
    28141 gaaattgtct aaagataaat ataagtgatg aagaggtgat gtgatgagat tagatatgga
    28201 tagtatgtta tatgtactaa tttatatgac attattggta tctatcattg gaagtatatt
    28261 ttatttttgt aaagacctct taaatcataa aattaagtat gttttaataa gagacatatt
    28321 tgtaaataga attacatatt ctgaagaacg gttgcctaaa cagtatatag tttttcagaa
    28381 atatgatatt tggaggtatt gtagtttgtt ttaatttgaa atgaatctat tataaattct
    28441 aaagtttaaa aataaaaagg agagaataaa atggatttca tttcaagtat attgcaagga
    28501 ttattaagta tattatttaa ttttacaggt gattttggaa tttctattgt acttataact
    28561 ttaatagtta aattagtact tatgccacta tcattaaaac aaagattttc tgtaaaaaaa
    28621 cagcaagaat tagctgaaaa aatggaacat ataaaagaaa agtataagaa tgatactaaa
    28681 gaattggaaa agcaaataca agtaagttct gtagaaagta tgaagagtat gctgggatgt
    28741 cttatagttt tggttcaaat gccagtagta tatgctttat atcatgtatg tttagatatg
    28801 acaaaggaat ttacaacaat tataattcca tggattgcta atttaggtac atttgataat
    28861 ttgtttatat taccttgtat atatacatta attatgttag cttcaaattt agtgaattat
    28921 ataccatatt taaaaataaa ttctcaagta aagtttaaca aacaaatggc tgtggtgact
    28981 accataacaa gtcttatact aactgttaga actccagtag ccataggtct gtactttata
    29041 acaagtgcaa tttattcatt aattgaagat atttgtttta gaatgtattt taatcgaaaa
    29101 aatagaatag tgacaaaata atataattat tattagaaat attttagcct gctaacatag
    29161 aaaaagttag taggctattt ttatctatga tatcaattcc attaagaata gtctaaaaaa
    29221 tactatcaaa atgctataaa aaatactata aaaatactat aaaaaaatag ttgaaaaata
    29281 aataaattaa tataaactaa aataaagcct acaaaaaaga ggtgataaat agaaatggct
    29341 aaaatactag ttgtagaaga tgaaaaaagg atgcaaagta ttattgtaga atatatgcaa
    29401 aagggaggat atacttgtat aactgctaat gatggagtcg aggcacttac aatattaaag
    29461 agcaacaata tagatttaat gatattggat attatgatgc cgtatttaga tggattttca
    29521 gtgtgtagag tttcacgtga aatgactaat atacctatta tcatattgac agctaaaggt
    29581 gaagaggagg acaaattaaa gggttatgaa tatggcgcag atgattatat aacaaaacca
    29641 tttagcccaa aagtactttt agcaaaagtt aatgccttgt tacgtagata cactactgat
    29701 attcccaaaa attcattgag tttaggaaag atttttatta tggtagcatc aaggcaagtg
    29761 tatgtagaag ataaattgat tgatttaaca tataaagaat ttgaattatt gcgtttattt
    29821 atggaaaacc ctaatcaagt tttttccaga gagaagcttt taaattgtat atggggatat
    29881 gattttgaag gaaacacacg aactgtagat acacatataa aaaccttacg caaaaagctt
    29941 ggtagtgaag gacaccatat agttacatta attcgttcag gatataagtt tgaggttaag
    30001 gaatgaatag tttaagaaag attatagaaa aactgaaaaa tgtagatatt tcaaattgga
    30061 ttaaacaaaa ttttggacta tattcagtta gaaaaaaagt gtttttactt tctaaattat
    30121 caggagcgtt aattatatta ttttatctgg ttgttgaaga attttcgata aactctaaat
    30181 ttgaattttg gatttggttt ataatactgg ttatatttat tatgagtatt gattttttat
    30241 tgggaaaatt tatttcagaa ccaatcacat ctataaataa atctgcaaaa agtatgtccc
    30301 aacttgattt ttctaatcca tgtactgtaa atactaatga tgaatttgga gaactttcta
    30361 ggagcctgaa tacaatgtct acaaatctac aacaggcatt atcagaccta gaaagtgcaa
    30421 atattcaact tgaaaaggat gtaaataagg aaagaatgct attggaacaa agaaaggagt
    30481 tggtagatgc aatttcacat gaaatgaaga ctccacttgg cattatacgt gcgtatacag
    30541 aggggcttat ggatgaagtt gatgaagaaa aaaagaaaaa ttatatgaat gttataatag
    30601 aagaaactga tagaatgaac aatatgattg tatcattatt agacttatct gcattggaag
    30661 caggagtttc taaacttaac ccagagcgtt ttgattttat agagtttgtt gaaactgtgg
    30721 caggtcgtct tttaattgat ataccagatg ccaattttta ttttacatat gatttgccag
    30781 agtatgaagt ttttgtagtg gcagataaaa tgagaatgga acaagtagta gagaatctaa
    30841 ttattaatgc aaagaagcat gttgtatata atggtaattt agatttatcg attacatgtg
    30901 aaaatggctt gcttttattt gaaatttata atgatggtag gattatagag caagatgaga
    30961 tttcaaagat ttggtctaag ttttatcgta gtgttcaatc tcagcgtaaa ggaggttcag
    31021 gtctagggct tgcaattgtt tcacaaatac ttacaatgca agggttagaa tatggagtga
    31081 ataatcgtga tagaggagta gagttttatt ttatgattcc aattgatgaa taatttagta
    31141 ttaatttata aattataagt atctgatgta aaatattttt ctttgtatac gtaagtattt
    31201 aaagagaata aaataaaaaa cttaatgaaa aagtctaata aaaagtttaa tgaaaagtct
    31261 tgttttcctg atgaaaacaa gattttttat taagcttttt attatttcac ttcgatttca
    31321 caagagagac actctgattt caccaaatag aaataaaata ggttaaataa attaaaaaat
    31381 agaagcgtaa ttaaataagg ttacaagggg gagtacttat gaaatggtat acatggttga
    31441 ctattatatt gataatattg ctttctattc cgcttaaaat taaatttata aaatggtggg
    31501 atagacgaca aaaaaatgat aaaggaggtt atgatagatg ataaaagtag gagagttaaa
    31561 attttcttat ggaaaagata aacagatatt gcatggactt aattttgaag taaaagaagg
    31621 agaaatcttt ggattccttg gacctaatgg ttcaggaaaa tccacaacac agaaaatctt
    31681 aaatggcgtt ttaaaaggtt acaatgggca cgtatccctt tttgggaagg aagttaaagc
    31741 atatacagag tctctatatc aaaagattgg tgttttattt gaatttcctt atttatatac
    31801 caatttaagt gcaattgata atttagaata tttttcgtct ttttatccta aaaatcaaag
    31861 gagagacata tatgaactct tagatttgtt agagtttaag aaagagttta taaataagcc
    31921 tgtatcatcc tactcaaaag gtatgaagca acgtattagc atggcaagag cattggtaag
    31981 taatccaaag ttgttatttt tagatgagcc tactagtgga cttgacccat ctggagctgt
    32041 actatttcgt aagattattg aagaagaacg aaaaaaagga acaactatct ttttgactac
    32101 tcataatatg ctagatgcag atttaatgtg taacaatgtg gcttttattg cagatggcaa
    32161 gattatggta atagataaac ccaaaaattt aaaaatgaaa aatagcaata ataaagttga
    32221 agtggaattt gtatataatg gaaatcgaga tattgaaatt gtagatatac aagagttaga
    32281 atctggaata acttttaaat atgatgaaat cttaagtgta cattcaaaag aacctacact
    32341 agaagaagtt tttattaaat acacaggaag gatgttagtg taatgtattc taaatttttt
    32401 accttagtaa aaaaagattt ttggatgatg atttatggta aattttttgt gctgacactt
    32461 ggctctctca tactttattc gtgttatatt aattttggtt atgtaaagtt tatggatgaa
    32521 gatagttatc acaataatgt atatctatat aatccagaga atataagcat gagtgcatct
    32581 ccattaatta atcaagtttc ttctataggt gaattaaaag caaaacttga tgaagataca
    32641 agtgcagttt gtattgatat gagttccagt aagccgaaag taattttaca tgaaagtatt
    32701 aaaggagttg acaattatag agctgactat gcattgtcat tattatcatc agaaaaagac
    32761 cataatagca aaatatcaga caacaatgca ataattcaaa ttaatgataa ggatacatct
    32821 gagataaaac aaagaaaaga catgacttgt gagattttat tctttgaaat tgtagctatt
    32881 ggatttttag ggattgcctc tttattattt aaagaaaagc agatgggagt aatgcgtatt
    32941 actggtattt tacctgtaaa aagaagtctt tttatattat caaagatatt tgcttttttg
    33001 atatgtgacc tatgttttac agttctttta gtagtattta atattggatt ttttgaagga
    33061 gtaaaaatac ttccacaagt attagttcaa gctagtattt tatcaattat tatgtctttg
    33121 attggaatag gatgctcaat gttattgaga gattttaagc aattttctct tatatatctt
    33181 gtggttgctg tattcataac tacacctata tttctatctg caaatacatc ttttgaaata
    33241 gattggatta aatatcatcc cttctatcat gtttatagtc agcttaaaaa tgcgtttttc
    33301 atgaattttt caaaagaatt agtttatttt gtagtgtgtt ctttgacaat aatagttcta
    33361 tttttacttg taaaatatat ttttaataaa gagttggtta gggagggata atcttgatag
    33421 gattacgtta ccagttaaag agtataaaaa aagataaaat gtgtataatt tccttctttt
    33481 taccagttgt tatggcgatt ttaataaatt ttatgggaac aattgatata tcttcattag
    33541 gagagatacg ttttggtata gtgaaggata cacttcctgt acaaacagag cagtggttga
    33601 agaagtatgg tatggtaagt gtatatgaca caaaaatgca tttaatacgt gaaattaagg
    33661 atattaaaac ggacacaatt ggagttgtta gagatggtaa aggaataaaa actatgcttt
    33721 atggaaatga gattaatgct ataaaaaatg ttggaaagac attgccagag atgtttagac
    33781 taaagggtga tttaaaagat gttgaggtta caatattgcc taaagatagt attttaaaag
    33841 agtttcagaa tgtttttatt gctatgattt taattactgc tatgtttatg ggatgtacct
    33901 ttaatgcaat gaatattata tctgaaaaag aagatggaat atcatttata aatgaagttt
    33961 tgccacagag tagaagtcag tatattatac aaaaagtatt tttgggtctt ttatgtgggt
    34021 gtttatcttc aatgataact gtatttatat gtttggatat ttcaatatta gatatgataa
    34081 tgatgttgtt actcgttgta ttttcagcat ttatttcatc acttataggt ttatttattg
    34141 gaaagttgtc agaaggtctt atggtaggaa ttgtttatat taaaatagta atgattttat
    34201 ttataggtgc tcctttgtta atatatcttc taggggttaa taccagcggt atagggaatt
    34261 tatgctatat aatgccatca gttgctacat ttaatggtat tatggagatt attaatggta
    34321 atgttattat tataaaagat attgtaattt tggttgtaca ttgtgtagta tggtttatga
    34381 tatatattat tttagataaa aaaagaaagt tatcttttag tagataactc agtataatta
    34441 taattgggac aatttaatag gtgatttaat atactttgta tagtgtaatt tatgtagtgc
    34501 aatttgtata ctttaattta tgtagtgtaa tagttagttt tattttataa ttattcaata
    34561 taatttagta agaaattcag tacaattaga gctacttagt ggaaaactta ataggtactt
    34621 aaactatata aatactttat tttcctccat ttgtactata aatttaattt atgatataaa
    34681 tggaggtatt attatatgag gtattattat ataaagttaa taaagatagt atcaaaatat
    34741 tatttgctat aaagagaggg actcaaaata aagataaacc tatttttcaa tgttgaaatg
    34801 ataaaatata tttgatttca tataaaaaat tttcttcata aattaaaaag tccattttaa
    34861 gccccctcat ttttaaccag ataaatcgag ttaaactatt taaatatatt actcggattc
    34921 atttgactta gatattcctt gcttgtaaaa ttgttttgaa taggggatag ccaaagcaaa
    34981 aaataaaagt ataatccctg ctacgattat taaattatta atagaaatta catctgccat
    35041 tggtccgaaa atcatcatac caagaggaat aaaaccagta tatataataa taggtaagct
    35101 aagaactcga cctagcattt gtggtgctac atgctcttgc aacatagtca tagctgatgc
    35161 ttgaataatt ggaattgaaa aattcgtaaa aaacataaat ataaaaaatt gccaagcttg
    35221 tgttacaaga cctagaataa ttgaaaaaat agcataggat aatattgcta gaaaaaaggt
    35281 cttatttcta tttttaaaac caccaaaaac tcctataatc aatccaccta aaaccataac
    35341 taaaaatcca atcatttcaa taatagacag gtatatataa cccttgccaa atgttcgttc
    35401 aataagaagt cctataagaa atcctgatgg aacacaaaga agcatgaatg ctccataagt
    35461 gaaaagcact tttctaatga acttatctga aaaacagtac tttatccctg ataccatatc
    35521 tgcaaaaaat gagacttttt cagtttgttt tggtatatta tgtttaggaa ttttgatgaa
    35581 tatcaataat acaatagcaa taattgcagt aaatacatca attaaaagaa cttgatttat
    35641 tttattacca aatccaagta ttgcagcagc tacaataggc actataaatt gtactataga
    35701 ctgtagacta ctatttatac cattaaaacg cattaaatgt tcttctggta caagttgagg
    35761 aatcattgcg tttacagcag gtgtttgtac accacttcct agtgaacgta taacagatat
    35821 tactgatatg gctattaatt cacttgttgt gccatttgat gacatcataa atagaattag
    35881 aactaatgta gatatagcaa ttgttgaatc tgatacaatt atgagtacct ttcgattgta
    35941 gtagtctgcc catacaccag caaaaaaaga aattatcatt tgaggtataa aggagcatac
    36001 agttaaaact gttacccata cacctgatga tgtctgaagt gtgacatacc atatcattgc
    36061 catttgtaca agtgatgagc caaataagga aatatattga cttgctaaaa aaagaatgac
    36121 ttttttcttc catgtagaat tagtattttt gttttccatc ctattcctcc tttgatttgt
    36181 gtgttgaatt tttcttgctc ttaaggataa cacacaaatc aaagtctgga gttagagtgg
    36241 agggctagga aaattgtttt ttcataattt gaagatgaga tttcatggtt tttgcattat
    36301 gttcagcttc tttcaatgaa gttatcagtt tgtcacacac tgagataatt tcgtcatcat
    36361 cttttggagt gtcaaggaca gttttaatat tagtattagg attagaagat aaagcattta
    36421 acattcgtaa aatagctgaa agagaatagt ttgcacaacg aagtgcgctt ataattttaa
    36481 gtcgttttat atcttcatca gtatatacac gataaccatt ttgttttctt tttactttta
    36541 acaaaccatt catttcccaa tttcttaaag tatctattgt aacatttaag tgttctgcag
    36601 tttcttttct agttaaaaag agtttatttg tttctggagt atttccattt aaaatctgtg
    36661 caacaatagt aagtgcttcc tcggcattag atttttctat ttctaattgt tgcaagtatt
    36721 gatttacaag actaatagcc ttatcaaagt ccctattcgc tgatgttctt acaatctcaa
    36781 gggcatcttt tcttaatccc ctttgtacca cttcaatttt caaggctgta cgagcaaatc
    36841 taaattgttc tatatgaaaa tcattaaaga tacgataacc attggtatta cgcttaggtt
    36901 taggtattaa gtttagttct tcatataaac gtactgtatt aggatgtaca ccaattaaat
    36961 ctgctacctc tgatgtcgtg taagtgtcca ttagtattca actcctttta gatatatagt
    37021 attagtcata ataacaccaa acaaaagtct ggtgttatag tggagggttt ggttatctaa
    37081 gagtaattag tttatctaag agtgattaat ctattataga agtaagtata tatttttcaa
    37141 taaactccca tctatcacga ggtttaaaca ctattttaga tggtatggct attactaagt
    37201 catattgtgg aatacaacaa atagcatttc ctccagttcc cattgctgaa taccacaaaa
    37261 caccatcttt tttacatatc caccacagat aaccgtattc attactattc attttaaccg
    37321 attcttcaat ccacgattta gatataatac gtttattatt ccaaatacca tcatttaaat
    37381 acagaaaacc aaatcgcaac atatctctag cactcaatgt aagtccccag ccacctatag
    37441 aataaccctg tctatctttt gcccaaccct taagattttt tccaaagaaa tcttcaaaac
    37501 caaaatcttt catatcataa tctggtataa ctttcatacc aataggttta aataagtact
    37561 cattagcaaa ttcacgagca gattttccag tagtttttgt tataatagct gaaagtaggt
    37621 gcgttccagc tgaagaatat ttgaaagtac caatttcgcc tttttgacca agttgattca
    37681 aggtatattt tatccaatct ttttgtttgc aaagtctttc taaagactca gtcatatttt
    37741 taaaagggta tggtgcagtc attgtaagta ggtgacgtat agttactgct tttttacata
    37801 tttcagaagg actacaagta tattcaggga aaaaatcaac aaccttttgg tcaacatctt
    37861 ttatatatcc tttatctatt gcaataccta ttaaggctga tataatactt tttgtaactg
    37921 atgcaacatg aaaagtatca tcagcattat atccattata gtacttttca aagacaactt
    37981 ttccttttct tgcaataatt attccattta tattactata ttgtgagatt attaaaggtt
    38041 ccagttgtga gagcttttct gaactcatgc caacacttga agggtctact ttttcaaata
    38101 ttaaatctaa gcaattgttt ttattcatta aagatacctc cctgttatag attttggatt
    38161 ttcttttaga gtttttggag aaccttctgc aattatataa cctcctttgt caccaccttc
    38221 tggaccaagc tcaacaatcc agtcacaaac atttaataca tctatatcat gttcaacaac
    38281 aattacagaa tttccatttt ccaccaattc atctaataat tgaatcaact tagaagtatc
    38341 atatagactt agaccagtag ttggctcatc caatacataa aggatattac catttcgttt
    38401 tttaccaatt tcttttgcaa gtttgattct ttgggattca ccaccactta aagtcggtgt
    38461 aggttggcct aattttatat accccatacc tatttgttct aatacattta agtgtgaaac
    38521 aatatttgga atatccttga agaaatgtac agattcagaa acactcatat ttaaaatatc
    38581 atgtatattt ttatttttat atttaataga taaagattca tcattaaatc tttttccctg
    38641 acattcactg catatatggt ctatacttag attatttcct aaccagacct tttcatatcc
    38701 actaccacta catttaggac aagcaccttt ggagttaaat gagaaatgac cagctgtaaa
    38761 tcctcgttct aacgattctg gctgacctgc aaatagtgta cgtatcttat cccatatacc
    38821 aatataagaa gcaggagttg agttcatatt tttgccaata ggagattgag atatctctga
    38881 atatccagaa atatgtttag taccaatcag tctatctgca atagtttcaa taactgcata
    38941 atcgtcttca tcacctattt cgctatctga agaatttatt tgattattga tagtatggtt
    39001 attaaattgg cttctaagaa gaggaagtag ggtgtctgat ataagtgaac tcttgccact
    39061 acctgatttt ccagatattc caaccatagc acccaaaggt aatgatacag ttacattttt
    39121 aagattattt gtatttgcat tttgaatagt tatacaaggt attttatcag tatgtgtaat
    39181 atttttattg cttgttctat taggcattgg atattttcca gatagatact gcccagtaag
    39241 tgatttgtcg gattgaagta gaccttctaa atctccttga tatactaatt gaccaccttc
    39301 aacaccagct tttggaccaa tatcaataat atgttctgcc atttcaattg tgtttttatc
    39361 gtgctcaact actatgaccg tattgcctaa atctttaagt ttctttattg attttaagat
    39421 gtctattttt tctgattcat gcaatccagc tgttggttca tcaagcacat aaattaatga
    39481 gtccatctct gaatcgagat gtgaatttaa aaatagtctt tgcatttccc caccactcaa
    39541 agttgacatt tctcggtata aggatagatg acctaatcgt gattctatta aatgtcgagt
    39601 tttggataaa atatcattta caagatttgt accaaatgtg tttaaattac cttggtttat
    39661 taagatttta aggaaatcat caatttctaa aatcgtcatt tttccaagtt ctccaatatg
    39721 ttttccgttt aaaagtactc tacgtgcttc ttcaccaatt ttaaaaccat tgcaatctga
    39781 acaggcagtc atagtataaa ctccatttaa atcctcaccc cttttgtaac gattgtgaaa
    39841 tacctttgat aaagaggaac tttttttacc attttcataa tgaccataga gtacttcatt
    39901 tcttatttct tcaggtagtt gtaagaattg tatgtctaaa taatcgttga attttttaga
    39961 aaatactctc aaatatcctg gtgtaatctt tgctctatct aagacttgtt ttagggtatc
    40021 atgcttatca aggactaatt tttcaatatt aatctcaaaa tatgcaccat gtccagagca
    40081 cttcatacac attccatctg aagagttgta tgagaaataa cttggtctta atcgttcttc
    40141 ctcataccca caattattgc aaattagatt atcgcttaca ggagtatcac acatagtaca
    40201 cattatttga ccttctactg aatacaatag tgcaagcatg ttgagaatat tggttcttgt
    40261 gcctactgtt gaacgaggat tgctctggcg aataatattt tgttgaacag ctatagtggg
    40321 agctaaacct tgaatattat caaacttatc ctcactatca atacctgcaa gtatacctaa
    40381 agattgcaga tattgtttac gaccttcctc aaatacaatg tcaaacataa ggctagactt
    40441 acctgagcca ctgacacctg ttgcaacaac aagcttatct ttggggatag aaacatctat
    40501 atttttaaga ttgtgtatct ttgctccatt aatttttatt tctttcattg gttcacctca
    40561 ttttatcaat tttatcaaac cgtaaaatag ggatatatta atccttaata caagaataga
    40621 ttaggtgagg ttattaaaaa ggttctatag gtaaatatat atcgacaaag tgtctatttt
    40681 gagggtgatt gtgtgggttg ttaagatata cttcaaaagg gaaggaggca cgtagcttat
    40741 aattaccatt tgatagccat tcattataca taaaatccca cgcatcacta tattcagatt
    40801 ttgaaatatc aaaatgtccc acaagatatt ttccagaagg gattatcata ttgccaagat
    40861 tgtcattctc tttgacaaca atgttatctg gaatggtcat gcaaatacta gtccttaatt
    40921 gatgctcctg tgtaaattgt ggattatcat gataaatggc taatatttgt gtgttttcaa
    40981 gtccaagtag attctgctca cttgcatatg tgaataaccg ttctaaaagt tttgggaaag
    41041 tactggttag attaccatat gaaccagtat atctagtgta aatagtattc atattatcaa
    41101 gttctagaat ttcaatttca cctttaacct ctctagtatt cttacattta ggttttgata
    41161 tgcttctatt gtactgagaa attttatatg gttctttgca attcttgcta taattgtttc
    41221 tatattttat agggcttatt ttatagtaat ttttaaatgc tctcgagaaa actgctgaat
    41281 cagtaaagcc gaagtagtgt gcaatatcag taacagtcat gtcagttcga tgaataagaa
    41341 aagcagttgc attttctagt tttagcctat ttacatattg tgccaaagtt tctttttcta
    41401 ttgctttgaa gattctatga aaatgaaatt ttgaaaaacc tgctatatta gaaagttcat
    41461 caagagaaag aggttcatgt aaatgttctt ctatatagtc ttgcactttg caaatacgat
    41521 ttagatattc ttttttactt ttagcatttg tcatatatag ccctccttat tgtgtaaaat
    41581 gtgtattaat taatttatag caatatagta ataaaatttt atgttttt
//
LOCUS       DAEPLF010000013        41261 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEPLF010000013 DAEPLF010000000
VERSION     DAEPLF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.013"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(19..426)
                     /locus_tag="KR314_000616"
     CDS             complement(19..426)
                     /locus_tag="KR314_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0327019.1"
                     /translation="MEIGKKLKNARVQSGMTQENVAEKINVSRQTISNWENEKSYPDI
                     ISVIELSNLYSISLDVLLKGDEKMIEHLEESTNVVKSNQKMIWAIIVNIIVVALLITL
                     NMFIPDNRYFLVGIFCLMVITSSALLYQIIKKF"
     gene            complement(593..976)
                     /locus_tag="KR314_000617"
     CDS             complement(593..976)
                     /locus_tag="KR314_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBH0327020.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNAGSPQNHTPQGQSKK"
     gene            complement(992..1318)
                     /locus_tag="KR314_000618"
     CDS             complement(992..1318)
                     /locus_tag="KR314_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBH0327021.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKKFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            complement(1553..4597)
                     /locus_tag="KR314_000619"
     CDS             complement(1553..4597)
                     /locus_tag="KR314_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBH0327022.1"
                     /translation="MKKMLKRLCTGFLALATVVTALPTTPVHAESKQYWTESKERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFRNGYKTRADASTRMSADQISDVA
                     LSIEYVKQYTDSHSGISKNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFSGARAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKASSNTSITDGNG
                     NYSIAGATYGVFADKDCTKQLATLTTDENGNTDVAEVKAGTVYIKELSAPAGYKVDKT
                     VYSLKVEAGTTATLNVSDTPKVTDTLIELFKIDMETQKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDGDGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTVEETKAPNGYLLDGAYMQAGDKSEQIKGLYVTQITEDGDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNENAVLVEGKLYKKNEVVKTIR
                     TDIEGITSTSADLLPYGKFRIVESEAPNGYLTDGAKPIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGENHVVVTDDNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEEMRCDSNKGFELIPPFEIVVSRN
                     NLVVDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENTELIIDGKRVENDYTFVADDEEMKVEISYTFNASALGGKNLVTFEELYD
                     FSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDVTFIDTVT
                     LEGLEVGTQYKLVGWQMLREENAELLINGKRVESDYTFIADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKPEEPEKPETPPTPEKPN
                     RPSDSPKTGDNTNLYGLLALLLTSGAGLAGIFFYKRRKMKKS"
     gene            5033..5650
                     /locus_tag="KR314_000620"
     CDS             5033..5650
                     /locus_tag="KR314_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327023.1"
                     /translation="MNVIKKFIFVILIINILGCGISLILQSSLGSDAITLLNEGIHIK
                     LGISYTIAGLIYNGTLLLIALLLNKKSLGLGSCVYVLVAGIFIDLYIYLLLDFNISSL
                     GIIIRIMSFIIGHILMCSGFAMLIKFDIGMSPLDAILIYIEGKFKYPYKVLKTIADLI
                     FLILGVYLGGNLGFGTIFSILCTGTTISIVSKMISVNKKRYYSSI"
     gene            5947..6699
                     /locus_tag="KR314_000621"
     CDS             5947..6699
                     /locus_tag="KR314_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0327024.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            6801..7721
                     /locus_tag="KR314_000622"
     CDS             6801..7721
                     /locus_tag="KR314_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBH0327025.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            7724..9640
                     /locus_tag="KR314_000623"
     CDS             7724..9640
                     /locus_tag="KR314_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBH0327026.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDGHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMISATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            9660..10529
                     /locus_tag="KR314_000624"
     CDS             9660..10529
                     /locus_tag="KR314_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBH0327027.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIGTVKC"
     gene            11039..12502
                     /locus_tag="KR314_000625"
     CDS             11039..12502
                     /locus_tag="KR314_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBH0327028.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVKVLEKEIVDGLEELERMLEV"
     gene            12506..13690
                     /locus_tag="KR314_000626"
     CDS             12506..13690
                     /locus_tag="KR314_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBH0327029.1"
                     /translation="MVDEAKILDVVSISRENVKKFDGERSYLSTGNLDFNKISNLEIV
                     TYENKPSRANQTVNIGEVIFAKMKDTKKTLVINKTNKNIIVSTGFYVLKPSKEILPQY
                     LYHYLNSSYFLNQKNRLSKGATQSALNNEGLANIKIRMYNLKVQEKVVRVLDKAQELI
                     DKRKEQIEVLDELVKSRFIEMFGTPSKNEKNWEISEIGKYLDVLTDYHSNGSYETLRD
                     NVTLLDTKGYALMVRTTDLENNNFEKGVKYIDEHAYNYLEKSKVFGGEVIINKIGSAG
                     KVYLMPFLNKPVSLAMNQFMLRFNEDKVNHIFLYNLLLTSYMESKIKEKVRGAVTKTI
                     TKDAVRKINIIVPPIRLQNQFANFVKQVNSLKFEMETSLKELEDNFNSLMQKAFKGEL
                     FN"
     gene            13723..14031
                     /locus_tag="KR314_000627"
     CDS             13723..14031
                     /locus_tag="KR314_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327030.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            14064..14444
                     /locus_tag="KR314_000628"
     CDS             14064..14444
                     /locus_tag="KR314_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBH0327031.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            14434..14862
                     /locus_tag="KR314_000629"
     CDS             14434..14862
                     /locus_tag="KR314_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327032.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            14912..18247
                     /locus_tag="KR314_000630"
     CDS             14912..18247
                     /locus_tag="KR314_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBH0327033.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEKLRKSLTEKRKVNTVNYDFKIDEVNEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGKYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMVTAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRKLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            18628..20691
                     /locus_tag="KR314_000631"
     CDS             18628..20691
                     /locus_tag="KR314_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBH0327034.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFND
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDVYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(20908..21474)
                     /locus_tag="KR314_000632"
     CDS             complement(20908..21474)
                     /locus_tag="KR314_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0327035.1"
                     /translation="MDTKKVTFSSICIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            21640..22161
                     /locus_tag="KR314_000633"
     CDS             21640..22161
                     /locus_tag="KR314_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBH0327036.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFNEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            22952..25030
                     /locus_tag="KR314_000634"
     CDS             22952..25030
                     /locus_tag="KR314_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBH0327037.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            25030..25809
                     /locus_tag="KR314_000635"
     CDS             25030..25809
                     /locus_tag="KR314_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0327038.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            25802..26611
                     /locus_tag="KR314_000636"
     CDS             25802..26611
                     /locus_tag="KR314_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0327039.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            26631..27113
                     /locus_tag="KR314_000637"
     CDS             26631..27113
                     /locus_tag="KR314_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0327040.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            27134..27538
                     /locus_tag="KR314_000638"
     CDS             27134..27538
                     /locus_tag="KR314_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0327041.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANDDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(27694..28854)
                     /locus_tag="KR314_000639"
     CDS             complement(27694..28854)
                     /locus_tag="KR314_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBH0327042.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            29062..30612
                     /gene="xylB"
                     /locus_tag="KR314_000640"
     CDS             29062..30612
                     /gene="xylB"
                     /locus_tag="KR314_000640"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBH0327043.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNIIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYINTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYKIYHRLYKRIYKSLKDDFKLLDE
                     VVNTKINN"
     gene            30708..32045
                     /gene="xylA"
                     /locus_tag="KR314_000641"
     CDS             30708..32045
                     /gene="xylA"
                     /locus_tag="KR314_000641"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBH0327044.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNVTDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            32567..33766
                     /locus_tag="KR314_000642"
     CDS             32567..33766
                     /locus_tag="KR314_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBH0327045.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            33933..34718
                     /locus_tag="KR314_000643"
     CDS             33933..34718
                     /locus_tag="KR314_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0327046.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            34887..36452
                     /locus_tag="KR314_000644"
     CDS             34887..36452
                     /locus_tag="KR314_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0327047.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFLFTFLIKKFDMKTPGREEDNEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKVVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            36466..37797
                     /locus_tag="KR314_000645"
     CDS             36466..37797
                     /locus_tag="KR314_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH0327048.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARILGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMIEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            37811..38299
                     /locus_tag="KR314_000646"
     CDS             37811..38299
                     /locus_tag="KR314_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327049.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            38313..38780
                     /locus_tag="KR314_000647"
     CDS             38313..38780
                     /locus_tag="KR314_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0327050.1"
                     /translation="MFFKRKKEASTINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            38783..39937
                     /locus_tag="KR314_000648"
     CDS             38783..39937
                     /locus_tag="KR314_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0327051.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMILSLFGCLAYGLIVLLNYLQE"
     gene            40255..41007
                     /locus_tag="KR314_000649"
     CDS             40255..41007
                     /locus_tag="KR314_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327052.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFIAIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPAVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            41008..>41261
                     /locus_tag="KR314_000650"
     CDS             41008..>41261
                     /locus_tag="KR314_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0327053.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKN"
BASE COUNT        15440 a         4875 c         7444 g        13502 t
ORIGIN      
        1 cataacaaaa tatcctcctt aaaatttttt gattatctgg taaagtaatg ctgatgaagt
       61 gataaccata agacaaaata ttccaactaa gaaataacgg ttatctggta taaacatatt
      121 taatgttatc agcaatgcta cgacaatgat atttacaata attgcccata tcattttttg
      181 gttgcttttt acaacatttg tactttcttc taaatgttca atcatttttt catcaccctt
      241 tagcaatacg tcaagactta tagaatatag gttgctcaac tctattacac tgataatgtc
      301 tgggtaagat ttttcatttt cccagtttga aattgtctgc cttgatacat tgattttttc
      361 tgcaacattt tcttgtgtca ttccagactg tactcgtgca tttttaagtt tcttacctat
      421 ttccatattt cgattgctcc tttcggtgtc attgtcatat attaaccctc aaatgtctat
      481 ccaatatctt cgacaaagcc ttttttcctt gtcaaaattg ttttacatag cttaaatacg
      541 gctcttttgg ctcattatac tgtaatttca gagtaaaatc tatgacctag atttattttt
      601 ttgactgtcc ttgcggtgta tggttctgtg gactgcctgc gttctggttg cctttattct
      661 tcaacacaat atcctctgcc tttacatacc agtccacatc tgcacccgta taagtttttc
      721 gtgatactgt atcggctact ggattgacaa gctccacgac tgcattgtac ggaaattccc
      781 ttaacggtac ttctggcggt acagacacgg ggataattcc accatgcaga ctgcacttca
      841 aatcatagat acgcttttta agctgggtac tcggtgtccc gtcctcattc tgtaagaaca
      901 catcacgcac cgctgtaaat tttaattctc caaatgtttt ttctttgtca ataacaaacc
      961 cgtttgataa tctcataaat tcttcactcc tttacttttc ttcaactgct acaatatcat
     1021 cagcaactaa tacataatcg gtatgtccca tatccccgat tgcgtaacct ctgccgtata
     1081 acttcggatt gacaagtttt actttctgct catacttgaa cttcttttcg ccagcctgca
     1141 cgggaatttc caccacaaca ttttcgcctt tctgtacgtc agaataaagg ttatagcttc
     1201 gtctggctaa gaccctacgg ttatttttat ctctttcaaa gataggctcg ctttcgcctg
     1261 caaattcaag agttccaaaa gactgtgcca tatctggcac gacatatttc atttccatat
     1321 tgttttacct cgtttctttc tatttttaat aaattgattg tgatttctta ttctggcggt
     1381 ttgggaagta ccagcaatcc cacagatagt aaagggtaaa atcaatgctt acgcaccctt
     1441 gactatccgt gttcttgcag gttgttggca gacaagccag aataagcgga agtctgccgt
     1501 ttgacagctt cggcggagag cgtgattttt cttttctcat accgttaccg ccttatgatt
     1561 ttttcatttt acggcgtttg tagaagaaga tacctgccag tccagcacca gaggtcaaaa
     1621 gaagtgcaag cagtccgtaa aggtttgtgt tatcgcccgt tttgggactg tcgctcggtc
     1681 tgttaggctt ttctggtgtc ggtggtgttt cgggtttctc tggttcttct ggcttttcct
     1741 taaaggtaat tgtctgtccc ttatcctcaa tatccttatg ttcggtaact ttcttcggtt
     1801 cgtctggatt gctcaaatcg tacaattctt caaaagttac aagctgtttg ccgtcaagag
     1861 aagtagcgtc aaaggtaaag gcaacttcca ctttcatagt ttcgctgtca gcgataaacg
     1921 tataatcact ttccacacgt tttccattga taagaagctc tgcattttct tcccttaaca
     1981 tctgccagcc cacaagtttg tactgtgtac cgacttccaa gccctctaag gttacggtat
     2041 caatgaatgt aacgtctttt ccagcttcaa gctctttgtt gccgtcctta tccgtagcag
     2101 tcgtatgtat cttgatgata cgttctgtaa ttaaaacggt ctgcccgtcg tcctcaatgt
     2161 ctttatgttc cgcaactttc acgggttcgt ctggattgct gaaatcatac aattcttcaa
     2221 aggtaacaag gtttttgcca cctaaagcag acgcattgaa tgtataggaa atttccactt
     2281 tcatttcttc atcatcagcg acaaaagtat aatcgttttc tacacgtttc ccgtcaatga
     2341 taagctctgt attttcttct tttaacatct gccagccttt gagctgatac tttgtgcctt
     2401 ttgtaagtcc gtctaatttg acagtatcaa cgattgttac ctcttttcct gcaaggatag
     2461 ttttttcgcc gtctttgctg gttgctgtgg tatggataga gatttctttt tcgtattcat
     2521 cagtcagcgt tcctaaatca accacaaggt tatttcttga taccacgatt tcaaaaggtg
     2581 ggataagttc aaaacctttg ttactgtcgc aacgcatttc ttcaatgatg taggtatcat
     2641 aaggcaacgc acccttgctg tcgtctggtt cagaagttcc aaaccacaca ccgtcctcgc
     2701 tggtctttcc tgcgtttgta ttgtgcttat gtgaagccca gtcagcagaa gtggaaaact
     2761 gcccgttatc atcagttacc acaacatgat tttcgcccgt cgtcttgctt gtgatcctaa
     2821 agggaacatc agcaagacgc ttgtgtgtgc ctgcaccgat ttttacaccc tcaatatctc
     2881 cacgcttaat ctgattatag atagaatggg cttcgtctgt taagtccacg atttttccat
     2941 tttctgtgat tgtaaaatca atcggttttg caccgtcagt taagtaaccg ttgggagctt
     3001 cactttcaac gatacggaat tttccataag gtaaaaggtc agcagaagta gaagtgatac
     3061 cctcaatatc tgtacgaatt gtctttacca cttcattttt cttatagagc ttaccctcaa
     3121 ccaataccgc attttcattt aaggaaatga tgtcaaaagc tgtatctttc aaagtggcac
     3181 ttccttgtgg ctttgtatcg cccgtttcta aatctcgttt ctgaattttg acaccgccac
     3241 ggataacctt gtctgatacg gaaaactggt tacttccaga taatacggca aggtcgccgt
     3301 cctcggtaat ctgtgttaca tataagccct taatctgttc ggacttatcg ccagcctgca
     3361 tatatgcacc gtctaacaag tagccgtttg gagcttttgt ttcttcaacg gttagcgttc
     3421 caagtggaag aaccgccttg ccgtcctgca tatagaagct gtcgccagat accttgtaag
     3481 cgtccgctaa ttttgtaata taatgggttg tcccgtcgcc gtctgtttca gcgattgtct
     3541 ttgtaaccca tgtacgagta gcttcggcag ggagattgtc tttgttatag aagcctgcat
     3601 aatacttcca tgtaaattcc gcacctgcta gagaagcgtt cccctgcgga ttgtctttct
     3661 gtgtttccat atcaatcttg aaaagttcaa tcaaagtgtc cgttactttc ggcgtatcag
     3721 atacattcaa ggttgctgtc gttccagctt caacctttaa ggaatataca gttttatcta
     3781 ctttatatcc tgctggtgcg gataattcct tgatatagac tgtgcctgct tttacctctg
     3841 caacatctgt gtttccgttt tcatcagtcg taagggtggc aagctgtttc gtgcagtcct
     3901 tatcagcaaa gacaccgtat gttgcaccag cgattgagta attcccgtta ccgtctgtaa
     3961 tgctggtatt actggaagcc ttttgaagtt tcgcatttcc gacattcagt ttcgcccaga
     4021 attgtcccaa ttcctgcccc tcgccagagt agatataacc gccacattca tagcgtcctt
     4081 tattttcttt gacaaaggct ctagcaccag agaaaacttc gtcctgcgtt gcctttggaa
     4141 tttcatcata agaagctcgc acgttatcac attgccagcc aagatgtacg ctcaatctct
     4201 gccagactac aagctgtctt aaaaggtaag cgtgattttt gcttattccg ctgtggctgt
     4261 ctgtgtactg tttcacatat tcgatagata aggcaacgtc gcttatctgg tcggcactca
     4321 tacgggtgct tgcgtcagct ctggtcttgt agccgttcct aaaatctgtg ttaatgtcga
     4381 tacagtaagc gtcctcgccc tcaacggtta aatgtccctc attaaaggta gaaccaatcg
     4441 aaccgtcatt cattactttt tcaacgatac cgacacgctc ttttgactcc gtccagtatt
     4501 gcttgctttc tgcatgaacg ggtgtcgtcg gtaaagcagt aacgacagtt gcaagagcta
     4561 agaagcccgt acacaatcgt tttaacatct ttttcataaa tctaatgctc ctttcatttt
     4621 gggtaataaa atagccgtcc aataagaacg gctggaaata gaaaaggaac gtcatgttag
     4681 gcgttccata gtctataggt tattcaattt tttcttgttt caatactgat gtgttgtacc
     4741 atatctttgc atatctagga aaacttgcgt atcaaggctc attcgttagt agtaaataag
     4801 tagtaaattg atgtgtttgt ttccttgaaa atgctgatag atactgtatt ttagcggata
     4861 atcagatttt tattgtgttt ttattgtaaa aaatgtaata agaactaatg gagaagaatt
     4921 aaaagaaaaa gtagatagtt tttaaatata aataatccct cttataaata ctatgttatt
     4981 aatacagttt tataagaggg attataatta taaataaggt ggtttattaa ttatgaatgt
     5041 cataaaaaaa tttatatttg taattttaat tataaatata ctgggatgtg gtatatctct
     5101 tatacttcag tcaagccttg gtagtgatgc aattacactt ttaaatgagg gtatacatat
     5161 aaagttaggt ataagttata caatagcagg gctaatatat aatggaactc tattacttat
     5221 agcattgtta ttgaataaaa aaagtttggg attaggaagc tgtgtttatg tattagtagc
     5281 aggaatattt atagatttgt atatatattt attattagat tttaatataa gtagtttagg
     5341 gattatcatt agaataatga gctttataat tggacatata cttatgtgta gtggatttgc
     5401 aatgcttata aaatttgaca ttggaatgag ccctttagat gctatattaa tctatattga
     5461 gggcaaattt aagtatccat ataaagtttt aaaaactata gcagatttaa tatttttaat
     5521 tttaggtgta tatttaggcg gaaatttagg gtttggaact atattttcaa tattatgtac
     5581 tggaactaca atttcaattg ttagtaagat gatttctgtt aataaaaaaa ggtattactc
     5641 aagtatataa attgataagt gagagtatag gataatgttt atgaatcatt ttcaataatt
     5701 cgagttaatt tgaggctgta agaattggag ttactcagtt tcagtaattg agtaactcca
     5761 atttttatta caaaatactc taataattac ataattaatt gaagcataaa attattatat
     5821 tataaattta catatataga cattgactaa atcttaattg tatataaaaa taagtagtta
     5881 agaggtaata tattatatta ctcgataaat ttagacaaat aaaacttata taaggggaga
     5941 taacatatga taattgagct aaacaaaagt aattctgaaa atctaacgaa gacggaactt
     6001 gaaataatta aatttataaa tgaaaatgaa tcaatattac cagaattatc tatagtagag
     6061 attgctcatg aaacatattc atctccagca accgtttcaa gagcaattag aaaatgtggt
     6121 ttaaatggat tcaatgaact taggtataga ttaacagtaa aagatgaaaa tgatgatata
     6181 caaaatatgg gggatataat aaataaatcc tttatagaag cacagagagt tatagaacaa
     6241 atatctttaa ctacactaat caatataata aaaactataa ataaatctag taagatatat
     6301 attttggcaa gagggcttac tgaatatgta gcaaaagagt ttagtcttaa attgcaatta
     6361 cttgatttta atgttgtttt tataaatgac cccaacataa tgaaaataaa atctaaatca
     6421 ataaaaaaag acgaacttat aatcatattt tcattgaatg gaaataccat agagcttgta
     6481 gaatcagcac agaatgccaa tttagttgga gctaaggtta taacttgctg ttgtagtgaa
     6541 agtgcaaaac ttataaagta ttcggattta tatattgtag gatacaaaca tgaacatata
     6601 tcaatttctg aatatgaagt atcttccaga ttgcccttgt atattatatc taggataatt
     6661 atagattata tggtcagata taataattct gaaaattaaa ttgaaaagtc tatgaaaatc
     6721 gattttctat ttgtgaaaag attttcatag acttttttat tataatgaaa acatacacag
     6781 cacaagcaag ggggaagaca atgatatata cattaactac gaatcctgca atagatatga
     6841 atatatctac taatgggata aagagaaaat tagtaaatcg tacatcaaat gctgtatatt
     6901 ctcctaatgg aaagggatta aatgtaactt ttgtactagg tcattatggt atagaatcta
     6961 agatattagg attttttgga ggattttcag gaaaatatat agttgaagaa agcgaaaaga
     7021 gaggatttga cgtattacct acttgggtag aagatacaac tcgtataaat atatttttaa
     7081 atgatggaag tgatgaattt aaatttgtaa actctgggtc atatgtaaac gaaaaacaaa
     7141 aactagatat gctagataag atacagtcat tagcagatat aagttactta tcaataagtg
     7201 gaagtctacc tcctggtatt gatgataatt actatgagga tatatttaaa atttgtaaaa
     7261 ataaaagtat aaagacaata ctcgatataa gttcaccaaa attaaaggag ttactacagt
     7321 ataatccata tttgataaaa ccaaatgatg aagaaatcaa agatatattt ggaattattg
     7381 taagagatga agaagatatt aaggatgtac ttaaattact gcatttaaaa ggtgctcaaa
     7441 atatattgct aactttagga gaaaaaggtt cttattttta taatggaaaa tcaatttatt
     7501 atgctagtgc tcaaccagtt acagtagtca gctctgcatg tgctggtgat tctgcattag
     7561 ctgctttttt aagtatatgg ttggaaaatc cagaacatat tgaagaagca ttaaaaaggt
     7621 cagcagcaac aggagcaagt gtggctgaaa gtaatggtat aggtaatttg gagaatgtgg
     7681 aagattatat aaaaaatata agagtcagaa aggtggtata aagatgggaa agaaaatgat
     7741 tctaggggta acaggttgtc caactggaat agctcatact tttatggcag aagaagcatt
     7801 aaaaaattca gcaagggaat tagggtgtga cataaaagtt gagacgaatg gagctatagg
     7861 tgtagaaaat aagttaacag caaaagatat cgaaatggca gatgcaataa tagttgcttg
     7921 tgataaaaat gttgatatgg acagattcaa tggaaagccg gtaatagaag tacctgtcaa
     7981 agaaggtata cataaggcta atgaacttat acaaaggtgt attgatggaa aagtagctat
     8041 tagaaaagga aacttatcaa gcagtaaaat tgcagaagaa ggaaatttat cgtttggtca
     8101 aaagttgtat aaagacttaa tgaatggtgt atctcacatg ttacctttag tagtagcagg
     8161 tggagtactt actgctatat catttttatg ggggatttat tcatttgacc caaattcaga
     8221 acaatataat caaatagcag ctacattaaa atctgttggt ggatattcta tgaacttgat
     8281 ggttcctgta ttggcagcat ttatagctca atctatatcg ggaagagctg gtatgttggc
     8341 aggacttgtt ggaggaatga tatcatttga tacaggttcg gggtttttag gtggaatcat
     8401 atcaggattt ttagcaggtt acttagttaa actatttgta catttgttaa gaaaattacc
     8461 acgacaatta gaaggtctta aatcgatttt tataattcct attgtcagcg taggtcttgt
     8521 gggtgtagca atgttgttac ttggtggacc atgttcagca cttaacaatg ctatgatgaa
     8581 ctttctatcg gggttacaaa attcaagtcc tataattttg ggaatagtaa taggatgtat
     8641 gtcagcattt gatatgggag gtccagtaaa taaagcagca tatgtaactg gaactatgct
     8701 tctaggacag ggaaattatt tatttatggc aggtgtatct gcagcttgta taacaccacc
     8761 attggtaata gcaatagcat ctactttatt taaaaataga tttacagaag aagatagagc
     8821 agcaggatta atcaattata tactaggtag tactcatata acagaggggg caataccttt
     8881 tgcagcaaaa aatccattaa aagtgttgcc agtacttatg attgcatcat caatatcatc
     8941 aattttaact tatataatga aaatagaagt accagctcca catggagggt ttttggttct
     9001 aggattagtc aataagccac ttttatgggt agggtgtata tttgtaggtt cattagtagg
     9061 tgctgtactt tatatgatgg ttacacctaa agtagtaaat gagaaaaata ctatagaaaa
     9121 tacaaatgca acaaaagatg gacataaaaa tgaaagtgca gaatcggaaa acgtatctaa
     9181 taatatctct ttatatagcg aagaaacagt aattttagat gttaagggaa aaaataaaac
     9241 agatgtaatt gatgaaatga ttggaatact tgataaatct ggtgttcttt tagataaaaa
     9301 gaagtttaaa gaagaaatcc ttaaaagaga agaaatatca tctacaggtt ttggtatggg
     9361 gatagcgata ccacatgcaa aaacagatgc agtaaaggta ccacgtgtag cagtaggtgt
     9421 atcaaaagaa gggtttgact ttgaatcaga agatggaagc ttggcacata ttatatttat
     9481 gatatcagct acagataatg gtgataattt acacttaaaa acattatctc aactatcttc
     9541 aaaattaatg aatgaagaat ttttaaatga gttattaaat agcaaaacaa gtagagaaat
     9601 agtatcaaaa ttaaataacg aagaaatcaa atcagtataa acatatagga ggagaaatta
     9661 tggcattagt gacaactaaa caaatattat tagatgcaca agaagggcat tatgcagtag
     9721 gtgcatttaa tgttgaaaat atggagatgg ttatggcagt aatagaggct gctgaagaac
     9781 ttaaatcacc agtaatattg caaacaacac cttcaacaat aaagtatgca gggctagatt
     9841 attatttagc gaatgtaaag gtagcatcag agaaggcaag tgttccagtg gcaatgcact
     9901 tagaccatgg ttcaagcttt ggactagcta tgcaggcact tagagttgga tatacttcta
     9961 ttatgattga tggttctcat gaaagttacg aagataatat ttcaatatct aaatctgttg
    10021 tagatgcatg tatgccttca gatatacctg ttgaggctga attaggcaaa gttggaggta
    10081 aggaagacga tttagatggt ggtgatggtg gagcatatac agacccatta gaagcaaaag
    10141 agtttgtaga gagaacaggg gtatcatctt tggcagtagc aataggtacg gcacatggac
    10201 tatataaggg agagccaaag cttgatttag atagattatc tgaaattaga gaggttgtat
    10261 cagttccatt agtattacat ggtgggtctg gaataccaga tgaggttatt aaggaatcta
    10321 taaaaagagg tatttgcaaa gttaattatg caactgaatt aaggattgct tatagcaatg
    10381 gggttaagga tgtgcttaat tctgacccag aggtaattga ccctaagaaa tatggtaaaa
    10441 aaggtttaga gtcagttaaa gattttgtta agagtaggat ggaagtttgt ggatgtgtag
    10501 gaaaagcaat agggacagtg aagtgttaat aataaaggga aagaataagt aagattaaat
    10561 taaggggtgt ctcaaaatga actttttagt tcatgaggca ctcttttttg tgtgaaatca
    10621 aatatatttt catcattctg acaataaaaa agaagattat atctattttg agacagcatc
    10681 ttattttatt ttttgattag gattgtagtt gagaaatttg aaataaggaa gatgatataa
    10741 ttaaataaaa aatttctttc taagtttaaa ttaatattaa aaagttataa tatggtaaga
    10801 gttatccagg tagatgactt ctatgatgga tatatgataa aataattttg tatggtatat
    10861 ataataatca taaaataaga gtattgcatt agttttttag ctaaaatatg taaatgggaa
    10921 aaataattta ataattgtgt agtaaaaact tggatttaac ataagacact aactattaag
    10981 gcgtacgact aaaatatata tttaaattat tttatgaaca ctggagggaa agtcatatat
    11041 gattacagga gaattaagga gtaaagtaga caagatttgg gagacatttt ggactggtgg
    11101 attaactaat ccattagaag ttatagaaca atttacatat cttttattta ttaaaggatt
    11161 ggatgaagta gaaacaaaaa atgaagcaga agcaactctt ttgggtgttt cttttgaaaa
    11221 gatatttgat gatgaacatc aacatttaag atggagtaag ttttctaatg aaggaaactc
    11281 agaaaaaatg tatgaaatag tacaaaatga agtgtttcct ttcataaaaa aattacatgg
    11341 aaataaagaa tcagcttatg caaagtacat gggagatgca atatttaaaa ttcctacgcc
    11401 tcttatgtta tctaaaatag ttgatggtat tagtaattta gaattaagta aggatagaga
    11461 tacaaaggga gatttgtatg agtatttatt atccaaagtg gctacagcag gtactaatgg
    11521 tcaatttagg actccaagac acataataga tatgatagta agacttatta aaccaacacc
    11581 agaagatatt atagtagacc cggcagcagg ttcggcaggt tttttagtat catcacaaca
    11641 gtacttaagg gataatcatt caagtctttt cttagtgcaa gggctgaagg agcattttaa
    11701 taatggtatg ttctatggat ttgatatgga tagaactatg cttagaatag gtgctatgaa
    11761 tatgatgttg catggcgttg acaatcctaa tatagagtat aaggattctt tatcggaggt
    11821 taatacagat aaagataagt ttacattggt gctagcaaat cctcctttta agggaagttt
    11881 agattatgaa gcagtgtcag cagatttatt aaaagtgact aagacaaaga aaacagagtt
    11941 attattcttg gcactttttt taagagtttt aaaaattggt ggaagatgcg catgtatagt
    12001 gccagatgga gtattgtttg gttcaacagg tggacataaa tcgataagaa aagaaatagt
    12061 tgataatcat aaattggaag ctataatatc aatgccaagt ggtgtattta agccatatgc
    12121 aggggtatct acagctataa tgatatttac taaaacaggg acaggtggaa ctgataaggt
    12181 atggttctat gatatgaaag ctgatggtta ttctttggat gataagagaa atgagataaa
    12241 ggatagtgat atagaggata ttattaagag atttgataat cttgatgggg aagtagatag
    12301 aaaaagaaca gagcaaagct tttttgttga tgtggaagag ataagagaga atgggtatga
    12361 tttatctatt aataagtata aagaggtagt gtatgaggaa gtagtttatg atgctcctag
    12421 tgttattata ggtagggtta aggtgttaga gaaagagata gtggatggat tagaggaact
    12481 agaaagaatg ttagaggtgt agagtatggt tgatgaagca aaaattttag atgttgttag
    12541 tataagcagg gaaaatgtaa aaaagtttga tggagaacgc tcatatctta gtactggaaa
    12601 cttagatttt aacaagataa gcaatctaga aatagttact tatgaaaata aaccttctag
    12661 agcaaatcaa actgtaaata taggagaagt tatatttgca aaaatgaagg acacaaagaa
    12721 aacactagtt attaataaaa ctaataaaaa tataatagta tcgactggct tttatgtttt
    12781 aaaaccatca aaagaaatat tgcctcaata tttataccat tacttaaata gtagttattt
    12841 tctaaatcaa aaaaataggt taagtaaagg tgctacacaa tcagctttaa ataatgaagg
    12901 attagctaat ataaaaatac gcatgtataa tttaaaagtg caagaaaagg tagtgcgtgt
    12961 tttagacaag gctcaagagt taatagataa aagaaaagag caaatagaag tattggatga
    13021 attagttaaa tcaagattta ttgagatgtt tggtacccca tcaaagaacg aaaaaaactg
    13081 ggaaatttca gaaataggga aatatttaga tgttttaact gattatcatt caaatggtag
    13141 ttatgaaacc ttaagagata atgtaactct tttagataca aaaggttatg ccttaatggt
    13201 aagaactaca gatttagaaa ataataactt tgaaaaaggt gttaagtata tcgatgaaca
    13261 tgcatataat tatcttgaaa aatcaaaggt gtttggtggt gaggttataa taaataagat
    13321 aggtagtgca ggtaaagttt atttaatgcc atttttaaat aaaccagtat ctttagctat
    13381 gaatcaattt atgcttagat ttaatgaaga taaagtgaat catatattct tatataacct
    13441 tttactaaca tcctatatgg aaagtaaaat aaaagagaaa gtaagaggag ctgtaacaaa
    13501 aacgataact aaagatgctg ttagaaaaat aaatattatt gtacctccta ttagacttca
    13561 aaatcaattt gccaacttcg ttaaacaagt aaactctttg aagtttgaaa tggaaactag
    13621 cttaaaagaa ctagaagata actttaactc attaatgcaa aaagctttta aaggagaatt
    13681 gttcaactga aatacaagta ttaaaatggg ggaataaaat ctatgaatca aatagagcta
    13741 gatttatatc atgacatggt aaatatctat aaagaagctg atttacaatg taactataag
    13801 ccaacaagat ttttacaact gataaataac aaaggagcct tattagctgc taaagaatta
    13861 atcaataaag aaggagctac tgaaggattt acaaggctat gggagtgtaa gagattagat
    13921 ttatcattag aagtattagt attaaaagaa aagtataaag agttgtttac tgatgaagaa
    13981 agagaaatat gtataaatag attaaaagat tatggatata aattcaatta agcaatatta
    14041 cctaataaac aagaggtgat taaatgagaa atataccaaa tgagattaaa aactcaataa
    14101 taaatgtatt tccaaaagaa aatactctag caatcataat atttggtagt tatggaacag
    14161 ataaacatac atcagaaagc gatatagaca tagcttggat tcctatcaaa aaagtaccaa
    14221 ttacagaact tgcaactaag acagaagctc taagaaatat attagacatt gatgtagacc
    14281 taaaaattgt tacagataat tatacaatag cacttaaaaa agctatttta gaaggtgaag
    14341 ttatttatga atctaaagat tttaaggatt atataaataa attctatttt gaaaacagtg
    14401 atgtaataga tatcttagat tggagggaca tgtatggtaa ttagaaacaa attatcttgg
    14461 aactcaatag aagaagtaaa tagggatttt ggttcttgct tatatgacct gcaaaacttc
    14521 aaaatcttat atgataagga agaaatgcct gagttatggg ctagatacat aaaagaaggt
    14581 tttaaaagct atatggtctc atttttagaa ttaacaaaag caatgttgta ttataaatca
    14641 attgatttaa acataaaatc taaaaacttt tatgactacc ttttagcttg tgagtatcac
    14701 aatttattac ctaaaaattc ttctatagtt atagaaacac ttagaaaatt gagaaatgat
    14761 gattctcatg gatatgatat accacagttt gaagatatgt acgaattgtt tacagaaaat
    14821 gaagaagtat ttgttagtat aagaaacagc tgtaagaaat agtttttaga gagatttatt
    14881 ttaactaagg gacaaaaggg ggagaaaagc cttgggtaat tttgactttt tacaaaagaa
    14941 gaaaaatttc aatagtttta atactgcttg cttagaagca gaaaaaagta tactagttag
    15001 cccatctact tgtgcaacta taacaagaag agctttagag cttgcagtaa aatggctata
    15061 tgctaatgat agtgatttag tattgcctta tcaagataat cttagtacgt tgattcataa
    15121 taatagtttt atagaactta tagactatga tatgttacct ttattaaaat atatagttaa
    15181 attaggtaat ttatctgtac atactagtgc gaacatagag agaggagaag caatactttc
    15241 acttaacaat ttgcatcaat ttgttagttg gatagactat tgttattcag atgaatatac
    15301 agcagaagac tttaatgaag atttattact tcatggggaa gagaaaagga cgagaccaga
    15361 cgagcttaaa aatctttatg agagattaag ttcaaaagat aaaaagcttg aagaagtaat
    15421 aaaagaaaat gagaaattaa gaaagtcact aactgaaaag agaaaagtta atactgtaaa
    15481 ctacgatttc aagatagacg aagtaaacga atttgagaca agaaaaagat atataaatat
    15541 agaattaaag cttgcaggtt gggaatttgg aaaagatata ggggaggaaa tagaagtaca
    15601 aggtatgcct aatgaaagtg gtgtaggata tgtagattat gttctttatg gtgaaaatgg
    15661 taagccatta gcagtagttg aggcgaaaag aacgagcaaa gacccgaaaa ttggtcaaca
    15721 acaagctaaa ttatatgcag attgtttgga aaagagatat agtcaaagac cagtaatatt
    15781 tttgacgaat ggttttgata tgtatatttg ggatgactat tcagatagaa aagtttatgg
    15841 attttataaa aaatcagaat tacaacttat gatagataga agaaagagta agaagtcttt
    15901 aagttctgta acaataaatg atgaaatatc caatagatat tatcaaaaag aagctattag
    15961 ggctgtatgt gaggctcttg aaaataagca acgcaaaact cttttggtat gtgctactgg
    16021 tacaggaaaa acaagaattg ctatatctat agttgatgta ctttcaagac ataattggat
    16081 taaaaatata ttatttctag cagatagaaa agcattggta aagcaagcta aaaaaagttt
    16141 tactaagcta ttacctaact tggcgttatg taatttattg gatagcaaag acagcccaga
    16201 agatgctagg atgatatttt ctacatatcc aacaatgatg aatgcaattg atgatacgaa
    16261 gtctaaggat ggaaagagat tgtttacacc tggtcatttt gatttaatta taatagatga
    16321 atcgcataga agtatttata aaaagtataa gtcaatcttt gattattttg attcttactt
    16381 aatgggtctt actgctacac caaaggatga gatagataaa aatacttatt cagtttttga
    16441 catggaaaat ggagtaccta catatgctta tgagtataat aaagcagtcg aggatggata
    16501 tttagtagat tatactagta ttgagtttaa gactaagatt atggaagatg gaattaagta
    16561 tgatgagctt tctgatgaag agaaggaaga atatgaaaat acatttaatg atgatgagag
    16621 tattggagac gaaataggga ataatgcagt aaatgagtgg ctatttaatt ctgacacaat
    16681 agatttagtt ttaaataagc taatgacaga ggggttgaaa atagaaggag aagaaaagat
    16741 tggtaagact ataatttttg caaagaatac aaagcatgct agggcaatag ttgaaagatt
    16801 taataaactg tatccaaagt atggtgggaa ctttgtaaaa gcagttgact atagtataaa
    16861 ttatgtagat tctataatag atgatttttc tgataagaat aaattacctc aaatagcagt
    16921 ttcagtagac atgcttgata caggaattga catacctgaa atcctaaatc ttgttttttt
    16981 caagaaagtg agaagtaaga ctaagttttg gcaaatgata ggtagaggaa ctagattatg
    17041 tccagattta ttaggtgttg atatggataa ggaaagattc ttaatatttg atttttgtaa
    17101 taattttgaa ttttttaaat ttaatcctaa aggatttgag ggaaataaag cagaaacact
    17161 tactgaaaaa ttatttaata taaaggtaag tatggttaaa gaacttcaag atattaaata
    17221 tatagaaggt aagtatgcag aacttagaaa agaattgtta gaagaactta taacttctgt
    17281 aaaagcttta aatgaggata gttatatagt taggatgaat ttaagttatg tacataagta
    17341 taaaaatgaa aatgtttgga gcaatattgg agcagtagca caaaatgaga taagagaata
    17401 tatatcacca ctcataactt catatagtga tgatgaattg gctaaaagat ttgatgtagt
    17461 tatgtataat atacaacttg catacttaca aaataataat gcaagtaaag ggataaggca
    17521 tgtaatggtt acagcagaaa aactttcgaa acttgggact ataccacaga tccagcaaca
    17581 aaaatatact attgaaaaag caatgacaga ggatttttgg gaagattcag atatatttga
    17641 ggtagaagaa gtacgtatat cattaagaga gcttattaag tatcttgaaa agtctagtca
    17701 aaagatatat tatacaagtt ttgaagatat gattgtagca gaagatagaa atgattctgt
    17761 atataatgca aacaatctta agaattataa aaagaaggta gagtattatt taaatagcca
    17821 taaagatgaa ttagcaatat ttaaattaag aaataataaa aagataacaa agcaagatgt
    17881 tgaaacgttg gaggaaatac tcttaaagca gttaggtaat tgtgatgatt ataaaaagga
    17941 gtttggagat actccagtaa gtcaattagt gagaaaacta gtgggattgg atagagaagc
    18001 tgcaaatgaa gctttttcag agtttttaaa taataagagt tttaatacta agcaaataca
    18061 ttttgttaaa cttatagtcg attatgtagt caagaatgga tttatagagg ataacaaagt
    18121 gttgatggaa gacccattta gaactgttgg aagtataatt gatttatttg aaaatcatat
    18181 tgaagagaga aataaattga ttaaaactat aaataaaatt aaagaaaatg catcagagat
    18241 aggctaatga tgttatttag tgcaaaatta ttgttaatta tgtagagaat aaatttatta
    18301 gtaggtagtc taatattggg ttgaaataga taactagata tggataatgt aaatatacaa
    18361 agagaggtat tgaataatta tgcaatatct ctttttaatt gataaataac tatgaattac
    18421 tcatgaagaa gattaaataa taatttttaa aattaagtat ttgtatattt tatagaataa
    18481 tcatgtattt actttaataa aataaaaata ggtataaaaa ggtatataat aaggctttca
    18541 tatacattta tgagtcaaaa ttttgatata atatatagat acatggagtt atgaatggaa
    18601 tttatttaac ttataggggg agttaatatg aaagcaggag aaattgaatt tcttagttat
    18661 ttagaggggt caaacaaaag ttttgtagtg cctgtatatc aaagaaatta taactggaag
    18721 aaagaacaat gtaaaagatt gtttgatgat ttagaggata taatagattc tggatttaga
    18781 actcatttct tagggtcagt tgtatctata tatagtatgg gaaaagaata tttaataata
    18841 gatggacaac aaagagtgac tactgtgtca atattattga ttgccatgtg tcaaataata
    18901 aaagagtttg gagaatcaaa aggaattatt acaacagaag aacagattaa ggagtgttat
    18961 cttataaaca agcactcaag agataaagat ggaaaaataa agttaaaacc aatagcagaa
    19021 gatagaaaag cttactatga actatttgaa gaagaaatga atacagatag aaattctaat
    19081 atagtttcaa attatttata tttttataat agaataaaag aagataatat atcaatagat
    19141 gatttgtttg atgctataca gaaattaact atagtagaaa tagagttaaa aaatggtgaa
    19201 gatgaccctc aattaatctt tgaaagtcta aattcaactg gcttagattt aagtgaagct
    19261 gatagagtaa gaaactttgt gttaatgggt agaagctcta agttacaaga ggaattttat
    19321 aataattatt ggtataaaat tgaaaaaaat acaagtagca acgtaagtag ctttataaga
    19381 gactatttga ctataaagga gagaagtata ccgaataaaa ataaagtata ttttagtttt
    19441 aaaaggtatg tatcagataa tggtatagat atagaaattt tattaaaaga tttacttaaa
    19501 ttttctaact attatagaga gatatcttat tcatgtactg atgataatga tgtaaacaaa
    19561 gttttgaaaa ggataaacaa acttgaaata tttgtatcat atccgtttat attagaatta
    19621 tttaatgatt ataatgaagg tataataaat aaaaaagtac ttgtagaatc ccttaattta
    19681 gtagaatgtt ttatattaag aagaatagta tgtgatgtac ctccgaatgc actgaataaa
    19741 gtatttatga acttaggaag ggaaattaaa aaatttagtg attataaaga aaactattct
    19801 aatatattaa gatatatttt tataaataag aaatcatctc aaagatttcc tactgacagt
    19861 gaattttcaa atgcttttga aactaaagat atatataata ctaagtcaaa gaataagtta
    19921 tatatacttg agaggttaga gaattataat aataaagaaa ttgttgattt agaaaattta
    19981 atcaataata atatgctaaa tatagagcat ataatgccac agacattaac aaataaatgg
    20041 aaggaatcac taggagataa ctataaggag atacatgata aatatttaca tacaattgga
    20101 aatttaactt tgacaggata taattcaaaa ctaagtaata aaacatttga agaaaagaaa
    20161 gaaatggaag atggatttaa agatagtagg ctttatctaa ataaatatat ttcatctata
    20221 gataaatgga atgaagaaga aataaaaaat agaactaaga tattactaga tagagctgta
    20281 gaaatttgga aatatccaag tgttacatat gaacctccaa gtaacagtga aaatatattt
    20341 acgttagagg atgatgatgt taattttaca aatgaaagta tagtatcatt tagtatatta
    20401 ggagagtcat ataaggctaa aaattggaca gaattttatg aacaagttgc tcttacttta
    20461 tatgatttgg attctgtaag gtttaacagt attgtagaca agacttataa aagagatttt
    20521 atagataata ttaagtctag agatgtatat aaattaagaa aaggtattaa aatatcagaa
    20581 aatatatatt tagaaacaaa tttaaataca gaggctaaat taaatataat tagattttta
    20641 cttaatgagt ttgatataga gttaaatgaa gtaatatttt ccataaaata atgaaatata
    20701 tgcatttttt gcgtaataga atattaatta tattaaaaat aaaatagcac ctcttgatat
    20761 agatttatag ctcaaattta tatcaagagg ttacgtttaa ttaaaataat acaaatacta
    20821 tctaattaaa ttattaataa aaatgttata taaaaatgag tatatagcat tttaaagagt
    20881 aataaatctt acataataaa tgatttatta atatgaacta gaagtatttt tactatattg
    20941 ttttataaaa cctttaggca ttttacttat aaaatatacc accataatac aagacaaagg
    21001 tttgtctact atattactaa gaatccttgg aaagaacgca gcagtaaata tcttctgacc
    21061 agactgaact aaaaaaccaa ccaataaatc agcaccactt ccacttaaac caccaaacaa
    21121 taaaactgaa ataggagtac caattaaagg acaaacaata gataaaatta ctcctgtaat
    21181 aatagcttct ttataaccaa aacctttttt cttagaaata tatccaacaa taagaccaag
    21241 aacaatattt acaagagcaa aaggtatatt tgtaggacct gatataacac ctaaaactaa
    21301 gtttgtacac ccaccaataa gagcacccca aaaaggacct aatacgacag ccccaagtac
    21361 agtaccaact gtatctaaaa ataataatgg tatatttatc atagatataa ccatacctaa
    21421 gacaatatta ataactatgc atattgaaga aaatgtgact tttttagtat ccaattataa
    21481 tacctcctaa aaaaatatat acatttatca tagtatagat tataaaagat tactaattgt
    21541 atatttctat atagaatatt ggaaatatag aacaaataaa taatataatt atcaaaatat
    21601 gtagtatcat taaaattaag aataactaag gagattatta tgaagagaaa tagagagatt
    21661 gtagtagtat gtcattgtat attaaattgt aattctaaag tagagggatt gtcagaattt
    21721 aagggagcac aaaatatagc gataaattta ataaataaag ggtatggaat aattcaacta
    21781 ccatgcccag aaatgattat gtatggaata aataggtggg gacatgtaaa agaacagttt
    21841 gacaatttgt tttttagaga acaatgtagg caaatgttaa tacctcatgt aaggcagttt
    21901 aatgaatatt taaaaaatga ttatgatata aaaagtatca ttgcaataga tggtagtcca
    21961 agttgtggtt ataataagac atgtagttct aaacgttggt ttggtgagct aagtggttgc
    22021 aatgaattga tggataaaat aaatgatata caaatggtgg atgggaaagg tatttttata
    22081 gaagagctag aaaagctact taaagaatat aatttagaga tacctatagt tggatttgac
    22141 gagcttagag aagataagta aagatataaa tatttattgt ctcattatga aacaccatta
    22201 tatttggcag aaaaatatga ctgatggaca gtacctgaat catttttaag tggagtaatt
    22261 agtacttata aagatgtatc tactagctga aagaaaatgt agagaggtaa taaatgaatt
    22321 gataatatat ataaaggagt gtctaaaagt gaacttttta gtttatgaga cactcttttt
    22381 ttatattaaa tcaagtatat taaatcaaat atattttaat cattttagca atgaaaaata
    22441 aatttatcta tattttgaga caacatcttt tttagtttta tttatttgtt gtcgtttttg
    22501 acttggtata caattatata ttttcttaaa taatttcata aattatttat aattataaaa
    22561 tccattgttt tgaacaatat ctaatatatt attatctgta tttataaggt cactatacat
    22621 attttcaagt ccaatattat ctacataata ggcaaatttt attcctgtat gtgtttaaaa
    22681 aaaactagat gtatataggt atcatcacag atattaaaag tataaataaa agatattaag
    22741 aacgactttc aagcaacata ttagaaaatt ttactaaaat ccggaaagcg attgacaaac
    22801 aaagtcaaat aaagtatatt attggtaagt tagtttaatg tacaaatgaa ttatatataa
    22861 aacctaattt ataaataacg atactaaacg acattattta acttaagcta cacaaagata
    22921 gaatattata ccattgattt aggaggtagc cgtgataaaa atatctgaag actatattga
    22981 aaaaagattt gacaatgaac tattgcgaat agaagcatgg ggaaaaaact ctttacgtat
    23041 taggtcgttt gtagaccaaa attttgttga tgaaaactat gctcttaacg aaaaacctaa
    23101 gttaaataaa gatgatatta caattaataa aaatgaagat ggaagtgcaa tcattaaaaa
    23161 tggaaagata aaagctgtct tagaccatag agatagaatt actttttata atgataaaaa
    23221 tgaaatatta ttgaaagaat atattcgatt aagagctgta aaacatgatg atggtggaga
    23281 agatgtaggg acaattgaga ttaccaaaga ctttaactct actctaaagt taaaatcaag
    23341 agaatatcga ccaagctata gtggagaatt tgaagtaact actagatttg aatctgagcc
    23401 ttctgaaaag atttttggaa tgggacaata tcaacataaa tttttagatt taaagaatac
    23461 tgttttggag ttagcccaaa gaaattctca aatatcagtt cctttttata tttctagtct
    23521 aggttatgga cttttatgga ataatccggg aattggaaaa gtatcttttg ctaaaaatat
    23581 gacagaatgg aagatgtttt ctacaaattt tatagattat tggattactt gtggagaaag
    23641 cccaaaagag ttaaataaaa attattctca agttactgga actgttccta tgatgcctga
    23701 aaacctttta ggactatggc aaagtaaact aagatataga acttcagaag aagttttaga
    23761 tgttgtgaag gaatattcta aaagaggaat taagctttca tcaattgcaa ttgactattt
    23821 tcattggcct aagcaaggtg aatataaatt tgacttagat tattggaaaa atcctaaaga
    23881 attagttaaa aaattgaaag aagagtattc agtagaacca atagtatctg tatggcctac
    23941 agttcaaagt gatgcagaaa actacaatga ttatttagaa aatggctatt tagtcaatgt
    24001 taatcgtggt gttagaatga caatgcaaat tcaaggaaat acagtctttg tagatatgac
    24061 aaatgaaaat gcaagagaat atgtttggga tagaatagat aaaaattata aacaacttgg
    24121 gattgactat tattggttag atgtagctga acctggctat tctgtgtatg attttgataa
    24181 ctatagatat aaaaaaggaa atgttttaag ttgtggtaat atttatccta ttgattattt
    24241 aaaaatgatt tatgatggtt tacataatga catagagtct gttgtaactc ttgtaagagg
    24301 agcttgggca ggagctcaaa agtatggagc tttagtatgg tctggtgaca ttgattctag
    24361 ttttgaggct tttaataatc aagtaaatac agggttaaat atgggactgg caggtatacc
    24421 atggtggact actgatattg gaggtttcca tggaggaaat cctaaagacc cagaatttag
    24481 agaacttatg gtaagatggt tccaatatgc tactttttca ccaattttga gaatgcatgg
    24541 agatagattg cctcatagta aaccattatc aaataaaggt ggaggctcaa tggtaactgg
    24601 agctcctaat gaaatatggt cttatggtga agaagttgaa gtaattctta caaaattcat
    24661 aaaaattaga gagagcttaa aaacttattt aaaaaaactg atgaaagaag cgcatgaaga
    24721 tggaacacca gttatgagaa ctttatttta tgaatttcct gaagatgata aaacttggga
    24781 ggtggataat acatatatgc ttggagatga aattttggta gctccaataa tgaattataa
    24841 agatagaagt agaaaagtat atttaccaaa aggacataca tgggaaaata tatttagtgg
    24901 agttagttat gaaggtggga aaacttatga agttgaatgc ccgctggaag aaattcctat
    24961 tttcctaaag caagattcca gttataattt taaagaacta aaaaatattt tgggagaggt
    25021 gaaatcataa tggagccaca attatggcaa ttattattaa tcgtactata tggatttttt
    25081 ataaactatg aaaaaaattc aacaatgttt ggaacatatc aaccagtaac agcaggtttt
    25141 attactggtc ttatcttagg agatataaat acagggttat atattggagg tacattacag
    25201 ctattatcat taggtattag taactttggt ggagcatcaa tacctgatta tcaaacagca
    25261 agtatagtag ccacatttat aactattacg actaagcaag aagcatctgt aggaatctct
    25321 ataggtattc cagttgcttt attgatggta caattagatg ttcttagaaa tactattggt
    25381 atatggctag ttcataaagc agaagatgga gctaaaaaag gaaattataa aaatataacc
    25441 tatatgcaaa tgttaggagt actattaaca gcagctacaa caggtattcc agttgctcta
    25501 tctgtaatat ttggaccttc tttaattaat accattttga aatatacacc tgaatggtta
    25561 acaggtggtt taacagtagc tggaggacta cttccagcag tagggatagg attattactt
    25621 agatatcttc ctgctaagga gtattttagt tacttagtaa ttggttttgt tttagcagta
    25681 tatatgaaag taccattatt aggtgtagct ttaattggtg gagcaattgc acttataatt
    25741 tacaaaaaga atttggaaaa tcaagaacaa caatatactg tagtaggagg tatggacgaa
    25801 gatgagtgat aaggtatcat taaataaatc aaatgaaaag gtgaaaaaaa gtgtcttatg
    25861 gcgctggttt tttactagtt ctgtttcttc aaattatgag aagatgcagg ccttggcata
    25921 ctgttatgca gtactacctt ttttaaaagt agtatacaaa gataatccag aaaagttaca
    25981 aagtgctgta ttaaatcatt tacaattttt taacactaat ccttgggttg ccccttatat
    26041 tttgggtatt aatgtagcaa tggaggagtt atcaagtgaa gagactgaag aggctgttgc
    26101 ttcaattaaa acaggcttga tgggaccagt agcaggttta ggagatagtt tatttgtcgt
    26161 tattccgtgg acaatttttg gagctatagc agctaatatg gcgatagatg gaagtccatt
    26221 tggaatctta ctgtggattg tagtgagtgt agcattgaaa ttattaagta taccactatt
    26281 taatgctggc tatacatcag gtactaagct aatatcttca attgaaaaaa gtttaaagat
    26341 tttaactgag agtacatcta ttttaggttt aatggttgta ggtgcattaa taccttcagt
    26401 tgtaaaagct aatgttgctt tgaactttca acaaggtgat tttacaatga aaggtcaaga
    26461 aatattagac caaattatgc ctgggttagt tcctgcaatt ttagttgtga ttgtttattg
    26521 ggctcttaaa aagaatataa aacctatata cttaatttta ggagtaatgg ttgtttcaat
    26581 aattttatat gcattaggta ttttaaaata aggaaaggat gatattagat atggaaatga
    26641 aaggtattaa aaatattcgt atagatgacc gtttaatcca tggtcaagtt gcaactatgt
    26701 ggagtaataa attgggtgtt acaagattaa tggtagtaaa tgatgcagta gctaacaatt
    26761 ctgttcaaaa acaagtttta agaatggcta cacctgcagg tatagcgtca tctataatta
    26821 cagaagagac tgctataaaa aatattactg caggaaagta tgaaggacaa aatgtattat
    26881 taattgttaa gtctcctgtg gaccttattc catttgtaga ggcaggattt aaaatacata
    26941 atataaatgt aggaaatatg tcaagtagaa aagatacaac agttctaaga cctaatatca
    27001 gtgttactga agaagagaaa gaagcattta aaagactttt agatgaagga attgaaatta
    27061 caactataat gacgcctgat gataagaaaa cttatcttgt agacatctta tagatttaaa
    27121 gaggtgtatt aaagtgaata gtattatttt gatgagtcat ggcaatatgg ctgaagaagt
    27181 cttaaattct gcaaaaatga taatagggga ttctatagat tatcctactg taaatatgaa
    27241 acaagatgat ggaatagaag gaacaataga aaaattaaaa aaagctttag aagtgtacaa
    27301 ggaaagttca gaaataatta taatggcaga tttactaggt ggaactccat gcaatgctgc
    27361 tttattagta gccaatgatg acagtcgaat taaaattatt tcagggctaa atttaggtat
    27421 ggttattgaa tcagcttttt tagatacaaa ggaattggca gcaaaattag agaaaatagg
    27481 taaagataat atttctatgg ttgaacaaaa tgcaagttta ttatctgatg atgagtagga
    27541 agattatatt aatatttaaa acaaaatact tgtgttaata cttaattgat atacaatttt
    27601 taaaaaatat agctaatctt gcataaacta tataataaat ctaattttag aatgtataat
    27661 ttaaagggtt atggctgtat atagtcataa cccttatatt tcattaatta aagttaaatt
    27721 ttgtatattc aaaaagctct tgatatttac ggcagagcca ccatatagag atgcctcaga
    27781 ccctaatgat gaaatttcta tatttattcc tttgttaaaa gagctaacta acatatcttt
    27841 tgtaatctgt aatatctcag ggatatcact aattatttgg ctatttaaat atattatttc
    27901 aggagcataa gttgtaatag cattatttat agctattgtt aagtaactac aaaactcatg
    27961 aataactttt ttggcattct ggttatcttc ataatatagt tgctttacta tatcagaatc
    28021 tattttagga atattttcta aagaagaaag ctgttcaaat accttttttt ctgaacaata
    28081 ctgttctaaa cagccacgat ttccacaagg acatagttta ccattaggca tgatgatagt
    28141 gtgaccaatt tctccactca taccatttct gccgctatat aatttattat ttattataat
    28201 gcctgaacca aatccactgt gaatactgag actaagtagt gagttatgta cagttgaaaa
    28261 agtattctca gctaaagctg ttaagtttgc ttcattttct atatgaatag gaaagtcata
    28321 ctttttgcta aggatgctat ataagtcaat ctcattaaga ttataatatg gagtaaacaa
    28381 tactttattt tcacaagtaa tcccatgtat agccaaagta agaccaatta ccttataagg
    28441 agtgtctatt ttggatatgt tataactatt tataatttca tctatcaatt gtataacatt
    28501 gtctttactt acttgtatat ctgttaattt cttagaattt ataatagttc catctaagta
    28561 agatagagaa gaaaatatat agtcgtatcc aatgtccata cttaaagaaa tccctgcaca
    28621 tttattaaat actaataata tgggttttct tccgccacta tgagtactat ttccaattcc
    28681 tatttcatga actagagatt catcaataag ttttttggta atagcggata tagttgcttt
    28741 attcaatcct atagtagaag ctatacttgc cctagaaata ggaccatttt ttataatttg
    28801 ttcaagtacc aatctttcat tcatttctcg aatagtgtat ttatcagtaa ccaatttgat
    28861 attcctcctt aaaataatat tgtaatactt tttacacaaa aataaaaggt tattttgcat
    28921 tgacaaagat aattaaatat tttattatta gttcataagt tagtttaata tactaacaaa
    28981 aataaagcaa gtaaaatata cctaaaatat aaaaaaatta ggataggaaa acgatagtta
    29041 tgaagtggca ttcaaggagg gatgaagttg gagtatgtat taggtgtgga tattggtaca
    29101 tcaggtacta aaacagtttt atttgataaa ctaggaaata ttataaaaag ttgtacatat
    29161 gaatatcctc ttatacaaga gaaatcaggt tgggcagagc aggatgcaaa tgattggtgg
    29221 aaagctgtag tcgaatctat aagagaagta gttcaaagtt caaatataag cagtgaatgt
    29281 ataaaaggaa ttggattatc agggcagatg catggtctag ttatgctgga caatgaaggt
    29341 aaaacattga gaaactctat aatatggtgt gaccaacgaa cagtaaaaga gtgtgaggag
    29401 ataactgatt tagtaggtga agaaagactg attgaaataa ctgctaatcc agcgcttaca
    29461 ggatttacag catcaaagat attatgggtt agaaacaatg aaccagatat atatataaat
    29521 actaataaaa tacttcttcc aaaagattat ataagataca aattgacagg tgaatatgct
    29581 acagaggtat cagatgcaag tggtatgcag ttgctcgata taagaaaaag agattggagc
    29641 gatgaagtac tagaaaagtt aaatatagat aaaaatcttt taggtaaagt ttatgaatca
    29701 caagagatta caggttatgt gactagagat gtagcaagtt tgacagggct taaagaagga
    29761 acaatagtgg taggaggagc tggagaccaa gcagcaggag ctataggaaa tggaatagtt
    29821 aaagatggag tagtttcatc tactataggt acatcaggtg tagtatttgc ttatacgaaa
    29881 gagccaaaaa ttgataaaga aggtagagtt catactttct gtcatgcaat accaaataca
    29941 tggcatgtta tgggagttac tcaaggagct ggattgtcat taaaatggtt taaagataat
    30001 ttctgtcaaa gtgaagtgga agtttcaaat tctttaggtg aagatgtata tgagattata
    30061 aataatcaag taagtcaagt accaacagga tgtaatggat tgctttactt accatatatg
    30121 atgggagaaa gaactcctca tcttgaccct tatgcaagag gtgtgttctt tggattatct
    30181 ccaatccatt ctaaaaaaga aatcgcaagg tcaataatgg aaggtgtttc atatagtctt
    30241 aaagactgta tggatataat agagaatctt aatatagacg ttaatgaagt aagagcctca
    30301 ggtggaggtg gaaaaagtaa agtatggaga cagatgcaag cagatatgtt taatcaagat
    30361 gtatatacta taaattcaag tgaaggacca gcacttggag ttgcaattct tgcattagta
    30421 ggagctggaa tatatgaaaa tatacaaaaa gcttgtgatg ctataataaa aacttcaact
    30481 aaattagaac caataagtga aaatgtagat acatacaaga tatatcatag attgtataaa
    30541 agaatataca aatctttgaa agatgacttt aagttattag atgaagtagt taatacaaaa
    30601 ataaataatt aaattagctt aaaaagaaaa ataaagatag aaaatagttt taaattaaga
    30661 aaacatacga catatattat atgtgtaaaa tattaggagg acatgaaatg agtgaaatat
    30721 ttaaaggaat aggacagata aaatttgaag gagtaaaatc tgacaatgaa ttagcattta
    30781 gatactacaa tcctgaacaa gtggtaggta ataagactat gaaagaacac ttaagatttg
    30841 caatgagcta ttggcatact ttatgtggag agggtaatga cccatttgga gtaggcacag
    30901 tagagagacc ttggaataat gtaacagacc caattgagat agcaaagatt aaagttgatg
    30961 caggatttga atttatgtct aagatgggaa ttgagtattt ctgttttcat gacagagata
    31021 tagctccaga aggaagagat ttagaagaga caaataagat attagatgaa atagttgagt
    31081 atataaaagt taatatggaa aagacaggta taaagttatt atggggaact gccaatatgt
    31141 ttggaaatcc aagatttgtt catggtgcat ctacaacatg taatgcagat gtatatgcat
    31201 atgcagcagc tcaggttaaa aaagctatgg aaataactaa gtacttagga ggagaaaatt
    31261 ttgtattctg gggtggaaga gaaggttatg aaactttact taacactaat acagaacttg
    31321 aaatggataa ctttgcaaga ttcttacaaa tggctgtaga ttatgctaag gaaataggat
    31381 tcactggcca attcttaata gagcctaagc caaaagagcc tactaaacat caatacgatt
    31441 ttgatactgc tactgtttta ggatttttaa gaaagtataa tctggataaa tacttcaaaa
    31501 tgaatataga agcaaaccat gcaacacttg caggacatac tttccaacat gaattaaata
    31561 tagcaagaat aaacaatgtt ttaggtagta tagatgcaaa tcaaggagac ctattattag
    31621 gttgggatac agaccaattt ccaacaaata tatatgatgc aacacttgct atgtatgaag
    31681 tgttaaagca aggtggaata gcaccaggag gatttaactt tgactctaaa gtaagaagag
    31741 catcttttga ggtagaagac ttattcctag cttatattgc aggaatggat acatttgcaa
    31801 agggattatt aatagctcat aaattattag aagatgaggt atttgaaaac tttactaaag
    31861 aaagatatgc aagttttagt gaaggtatag gtaaggatat agtagaaggt aaggttggat
    31921 ttaaagagtt agaatcttat gcactgcaaa tgccagttat aaagaataag tctggaagac
    31981 aagaaatgct agaagctata cttaacagat atatatatga agtagataca atttctaata
    32041 agtaattgtg tagtaattaa acttggatgt ttgaataaaa taacaggata tgttcttgtt
    32101 tttattctaa aacggacaaa gataagtatt tgtaaaaatt ctcataatca tgataaatta
    32161 aggatataga cttaagtata attagtttat atccttaaaa tttctataaa aaataatcat
    32221 ttctatagtt atcaatattt ttaattatca atacctttct caactaataa ataaaaatca
    32281 gtaaagtatc taaatttaag tcattataat ggataaattt tttagcatat cttttttagt
    32341 atatcactga taagaattta ctttcaggat atagcagtaa aatagatatt tgaatataat
    32401 tgttatttta taactttcat gaaataattt tgaaaataca aataaaaatt tttaaataga
    32461 ataatagtgg aatatatctt caagaatttc ttttaactgt ttaggtgata ataaaataat
    32521 gatacaatgt attaaaatat aaaatctgaa agtggatgag aattacatga aaaagaaagt
    32581 attattttta gtaattattt tagcatttat aatcttatta ggtaattcta cttattctca
    32641 aaagaatcag ttgtctgaag aggaaaaggt ggatgacttt aattatgtgt ataatgttgt
    32701 aaagacagga tatccatatc taggtgtaaa taagagactt aataatattg attggttagc
    32761 aaataaggat gaatatacaa aacgaataaa aaacactgaa aatgatgaag aatttataga
    32821 agaactgtca tctatattat ctgatttaaa taataaacac acagaagtaa tagataataa
    32881 gaaaagatat gaacttttta agaaatcata ctctaataat gattggtatg attttcttaa
    32941 tgataaaaaa gttgtggata gatatgactc tatgaatctt aaaataaaaa tgccagatga
    33001 tatttttgta aaaaaagaat taattttaaa ggatgttatt aagggtcaga ttggatatat
    33061 gtatttacca tcaatgtcat caaagaatgg ctctataaat aatgatttaa aaataattgg
    33121 agattatatt aacactttag aaaaacataa agctttaatt attgatataa gaggtaattt
    33181 aggtggaagt gataggtatt ggcagggaat cgtatctaaa ctcataaaaa atgatgtcaa
    33241 aataaatggt tatagaattt atagaaataa taatgaaata gtaaaaaaat atacaaatgc
    33301 aagaaatata aaattaaatc ctgtagaaaa tttaccagca agtgtaaagg aaaatgcacc
    33361 taaagaaatt ataaaagatt ttaatgattt tgaagatact tcctatacta taaaatctac
    33421 agatgattca aaatttaagg gtaatatata cttacttgtt gatagaaaaa tatattcatc
    33481 atcagagtca tttgcaatgt tttgtaaaga aactaaattt gcgactttag ttggtgaaac
    33541 tactggcgga gatggagggt gtatagaccc tgtattgttt aatttaaaaa atagtggtct
    33601 aatagttaga atggcaagtt gtatgtattt aaataagaat ggaatatgtg atgaggaatt
    33661 taaaactatt ccagaattta aagtaaaaga ttgtaaaagg actgctgatt ttaaagatga
    33721 taattgcata agaaaagcac tagaacttga aaatattaat tattagataa aagaataaaa
    33781 ttaaaactac atttttttaa acatcaatta tagcagtatt ttttatagtc aattttatgg
    33841 gctaaatagt aaatataaga atacgctgta atttaatgtt gaatattggt ataatattta
    33901 tgacgatttt taattaaagg ggataatagt ttatgagatt agaagaaatg atttttttaa
    33961 attataataa attgaatgat acagatttat taatatggaa atatataact gcaaataaaa
    34021 aacattgtag taagatgtca ataaatgaac ttgctatgaa atgtaatgtg tctcgtgcaa
    34081 ctattacaag atttgttaaa aaattagatt taaaaggttt cagtgagttt aaagtattat
    34141 taagctggga atcagaaaag gttcatgtca tagaaaatca gtcatttgat attgcatgta
    34201 attcaataat taagtatgtt gaagatcaaa aaaataaaga ttatgatgca ttgtgtaaac
    34261 ttctttatga atcaagaaca atatacgctt atggttcagg tgatattcaa aatgtggttg
    34321 caaaacagat aaagagaatg ttcttatcat gtcaagaaat aatatatgat tttgagggaa
    34381 gaacatttga tgattctttt tacaatattg tcagtaaaaa tgatgttatg attattatat
    34441 ccctaagtgg tgagaatgag aatacactta atattgctag aagattaaaa ttaataggaa
    34501 ctaaaataat ttcaattaca gagtttaaaa ataatacatt gactgattta agtgatgaat
    34561 caatttatat ttcagcgact aatatctcat ttttacaaat gcaccctagt tataaattga
    34621 caatgttata ttttatattg attgagttat tatttattaa atatagtatt tataaaaggc
    34681 atagaatgat tacagaaggg attgattgca aattgtagtc attctttttt ttatgagaat
    34741 ttgaacataa aaatgtgcaa aaacttattt gactgtgaat aagctccaaa tgtaccctaa
    34801 agattgtata tatttattaa cctgttaaaa taatagatat ctaaggccaa agataaacta
    34861 aaaatatgag gaggggagta atttatatga tgaagaaaat acagcgtttt ggaggggcaa
    34921 tgtttacacc aacactatta tttgcttttg ccgggataat ggttggattc tcaattgtat
    34981 ttcaaaatca gtcaattatg ggttcacttg ctacaccaga aaatatttgg taccaatttt
    35041 ggggtgtaat atctagtgga gcttggatgg tatttaatca attaccatta ttatttgcta
    35101 tttctttacc aatagcactt gcaaaaaagc aacaagcaag agcttgtatg gaggctttag
    35161 caacgtattt gacttttaat tattttgtag gttctatgtt aagtttttgg ggaaaaagct
    35221 ttggagttga tttcgcagct gaaataagtg ctggaagtgg acttgtaagc attgctggta
    35281 ttaaaacatt agatactggt atggttggtg ctttgttaat atcaggtatt gtcatttata
    35341 ttcataatag attttatgat aaagaactac cagattttat tggattattt agaggctcat
    35401 cacttgtagt tgcaatttgt ttctttgtta tgataccagt tgcattatta acttgtttta
    35461 tttggcctca tattcaaaat gttattagac atttgcaaac gttttttata aatagtggaa
    35521 atataggagt ttggtgttat gcattcttac aaaagatttt aataccaact ggattacatc
    35581 attttgtata tgcaccaatt tgttatgata gtgttgttgt tccaggtggt acatcagtat
    35641 attgggctac tcatattcag gactttcaaa cttcagctaa gactctaaag gaaatgtatc
    35701 caatcggatt ttcattatct ggattatcga aggtgtttgg ttcattaggt gtatttggtg
    35761 ctttctatgt aactgcaaag ccagaaaaaa agaaaaaagt attaggttta atgattcctg
    35821 ctactcttac tgcagtatta actggaatta ctgagccgtt agaatttaca tttttatttg
    35881 tagcaccact tttattccta gtacatgcat ttttagatgc atgcttgcaa acgatttcgt
    35941 ttgccttagg agttgttggt gattttggtg gaggaattat taactgggtt gtattaaatt
    36001 ggcttcctct tggaatgtat cactggaagg tatatgttgt tcaagttgtt gtagggatta
    36061 tattttcatt tatctggttc tttttattta ctttcttaat taagaagttt gatatgaaaa
    36121 ctcctggtag agaggaagat aatgaagaaa caaaactata tactaaaaat gaatacttag
    36181 aaactaaaga tgaaaaagga aataaattga gtaaagcctc tcaacaagca tctgaataca
    36241 taaaattagt tggtggagca gaaaatgttg tagatgttac aaactgtgct acgaggttga
    36301 gattaacctt gaaggatgac tcaattattt ctaaagaaga agattttaag gtagtaggtg
    36361 cacatggttt agtgcataat ggaaaagcta ttcagattat tattgggctt tcagtaccaa
    36421 gtgtaagaga agaatttgaa aatttattat aaaggagaag gaaagatgga aagaaaaaga
    36481 cagaggatac taattgctgg tgggggtagt acttatactg ctggaattgt aacaatgtta
    36541 attgaaagtg ttgctaaatt tcctattgaa tcaattaaat tatatgataa taatgatgag
    36601 agacaaagaa aagttgctga agcatgtgca ataattgtta gagagaaaaa tccagaaatt
    36661 aaatttagtt atacaacaaa tccagaagaa gcatttactg atattgattt tgtaatggca
    36721 caaattcgtg ttggtttata tgctttacgt gaacaagatg aaaaaattcc attaaaatat
    36781 ggggttgttg gtcaagaaac ttgtggggca ggtggaattg cctatggatt aagaacaata
    36841 ggtccaataa tcgaaatgat tgattatatg gaaaaatatt ctccaaatgc ttggatgctt
    36901 aattattcaa atccagcagc tatagttgca gaagcatgtc gtgtattaag accaaattca
    36961 agaattatta atatttgtga tatgccagta tgtttagagg aaatatttgc tagaatttta
    37021 ggtttaaatt ctcgtaaaga ctttgatgtt agatattatg gattgaatca tttcgggtgg
    37081 tggacaagta ttaaggacaa agaaggaaat gatttaatgc ctaaattaca ggaatattgt
    37141 gctaaaaaag gatatgagga atttacacct caaggtcaac acaaagaatc tagttggtta
    37201 gaaactatga gagcagcaaa ggacttatta gaaatagaac cgactacatt acctaatact
    37261 tatttaaaat attatttaat ggctgatgaa actgttgaac atgctaatcc aaattataca
    37321 agagcaaatg aaattatgga tagaagagaa aaggatacat tcgaagaatg tgaaagaatt
    37381 ataaaaaatg gcacagcaag ggatacatgg tttgatgcaa gtgaacattc tcaatttatt
    37441 gttgaattag cttgtgcatt agcatttaat actcaggaaa gatttttact gattgttcct
    37501 aataatggag ctattgaaaa ttttgcagat gatgcaatga ttgaaattcc ttgccttgtt
    37561 ggaaaagata tggttgaacc tatgtctata ggtaaaattc caactttcca aaaaggttta
    37621 atggaacaac aagttgcttc tgaaaaatta gctgtagaag catggataga aaaaagttat
    37681 caaaaattat ggcagtcgtt tactatgagt aaaactgttc caagtgcaaa agtagcaaaa
    37741 gaaatcttag atgaaatgat tgtggctaat aaggatttct ggccaacttt taaataagga
    37801 gaacaaaaac atgataacaa ataaaataaa aaatataaca gcagtattaa aagatgaaaa
    37861 tatatttctt tcagtggtta tggtttgttt tctaatatta cctgttttat atatagtgta
    37921 tttagaattg acatcattat ttacaaacat ttcattttca caacttttaa acaatgatgt
    37981 taatgcaagt ttaaatttaa tctctaactt tagtgcagta tacagtgcat atgttattta
    38041 caatataaaa aagaataaag agataaaaga aaacatgatt gctttctatt tagtcttatt
    38101 agggcagata ttccaaattt acacaataac tattgttatc atgtttacct atatttataa
    38161 atttgttgga attaaacaaa tcataagcag tattaaagaa ctacaatggg gaaagagcta
    38221 taaggctctt ttttcctcaa ttgcagtatg cttaatatcg ttatttgtaa tgataattag
    38281 aataaagtta attatgtaga ggagtgtcta taatgttctt taaaagaaaa aaagaagcaa
    38341 gtacaattaa ttcatacatt gatggaaaaa taattaatct tggagaaatt cctgataaag
    38401 tattttcaac taaggtaatg ggagatggat ttgctatgat agcaaaggac aatattctta
    38461 aatcaccgac taaaggagta ataagtttta ttgctcaaac taaccatgct ataggaatta
    38521 aattgcaaaa tgatgttcaa attcttattc atattggatt ggatagtgca acaacaaaaa
    38581 aagaaaactt taaattagaa gttaaacaag gtcaagaagt taatatgggg gaaaagttag
    38641 tagaaattga taataagtta cttgaaaata ctttatatat tcctttagtt attgtagaga
    38701 atcctaatac ggtaaaatat catctttcaa acttaaatca ggaaggaaat agtggtatta
    38761 aagttatcaa ttttgattga ttatgattaa taaagcgatt aaatttaaat ttatcatatt
    38821 ctattttttc tcatatatta tttttgcctt tgcttcaaca aaattcacac ctttcttatc
    38881 aaaattaggt tactcagcgt ttgaaagagg aattatatta tcgagctatg caataaccaa
    38941 tatattattt caattattat ttggagtatt agctgataag tatcaaacaa tgaaaaaaat
    39001 tgttttaatt agtttatcag tttatggaat tgcatcagtt attatgtttt ctactgaatc
    39061 tgcaactttt ataatatact tattacttgt tgctttatca ggaggattat taaatacttg
    39121 ctgtagttta tatgatacat ggataatagg ttgtggagat aatattaata attatttgtc
    39181 atttattaag acttttgggt cgattggatg ggctattgga agtatggtag catcttactt
    39241 gattcttaga ttctcatatt ttggattatc tgtagcgatt ggtttaattg tagtattact
    39301 tattataaat atactattat tacctgatat tgaaaagatt aataagaaaa taaatgtaac
    39361 taataaagat attattgaat tattaaaaga taaaaaatat atcttactag tatgtatttt
    39421 attcttatta tactcaatgg tggtagcaaa taactgtact gtaatagata aaatgatagc
    39481 tttaaatgct acaaattctc agatatcttt aaagtggtct ttacagtcat tattagagat
    39541 accaacatat ctggctggtt cttatttatt aaaaaaatat agcagtttaa acttattaaa
    39601 attaagtgct attatgataa cagttcagtt tctattattt gctttaacaa atgattcaaa
    39661 tatcattatt atactgtgtg tttttcaagt gttttctact cctttaattt taattacttc
    39721 aaaaagatta atatttgaag tttctcctaa aaaacttaga agtagtagcc aattaatagc
    39781 tttaagtata tttatgggag gttcatctct gattattcca actgttgctg gttttttgag
    39841 tattaatatt gggtataatt atacattaat gatattaagt ctttttggat gcttagctta
    39901 tggcttgata gtcttattga attatcttca agaataattg ttttaatata tagtagtaat
    39961 atcacttgtt tatagtacct gtatttgcca caatcaaggt tgtatttttg gttttttata
    40021 gagaagaatt attgtataga ttagaggctt cttaggggct tcttttcttt gtttatacaa
    40081 aaaagtgaat catgctttta ttttaagata tttccactat aattataata gtcttccata
    40141 tgttacaaaa gtgtaatgct aacagttaaa atgtaatatt tacttaagga tttatttata
    40201 taaaaccttt aaaataaata tatattaaaa aattagcaac tggaggctat tattatgaaa
    40261 gaaatattaa aaataaaaaa tatatcaaaa gattatggaa taaaaggatt taaaacaaat
    40321 gtgttaaaaa atatatctct tacagtcaat gaaggtgatt ttatagcaat aatgggtcca
    40381 tctggtgcag gaaagacaac attgctaaat ttaatgtcta cacttgacaa acaaacttca
    40441 ggagaaataa tactagatgg aattaatata tcaaaagtaa aaaataatga actatcaaaa
    40501 ttaagaagag aaaaaatagg gtttatattt caagactata atctattaga caatatgaaa
    40561 ctaatggaca atatagcact tcctctagca ttaggaaaaa agaaaagcaa ggagatagaa
    40621 gctaaggtat tttctatagc taaaaaattt ggattagaaa atcatttaga taaatatcca
    40681 tatcagttat caggaggaca aaaacaaaga ggcgcagcag ctaggtcatt aattacaaat
    40741 ccagcagtca tatttgctga tgaaccaaca ggtgctttgg actctaaatc agcatatgaa
    40801 cttttagaat cattagaaaa aataaacaga gaaaacaacg ctactattat aatgattaca
    40861 catgaccctc taacggcaag ttattcaaat gaagtatata tgataaatga tggaaatata
    40921 aagtgtaaat taaacaaagg aaatagtaga aaagaatttt atggaaaaat tatggatatg
    40981 ctagcctcta tgggaggtga gatgtaagtg agtatacttg gaattgctta taataatttt
    41041 aagagtaaca taagaactta tctagcattt tttatatcta tggttttttc agtagttgtg
    41101 ctgactaatt ttgaattatt aagatatgga gatgctataa atgtgttaca agaggaaaat
    41161 aaaaaattta cactatcagt gttagtagct gtaataatga tactatcagt atttttattt
    41221 ttctttatat ggtatgcaac aaatatattt tttaagaata g
//
LOCUS       DAEPLF010000014        40151 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEPLF010000014 DAEPLF010000000
VERSION     DAEPLF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40151
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.014"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            37..687
                     /locus_tag="KR314_000651"
     CDS             37..687
                     /locus_tag="KR314_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0327054.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSGDDELTMKMISSIEYIIKHAK"
     gene            complement(844..1863)
                     /locus_tag="KR314_000652"
     CDS             complement(844..1863)
                     /locus_tag="KR314_000652"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBH0327055.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVINKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(1894..2508)
                     /gene="plsY"
                     /locus_tag="KR314_000653"
     CDS             complement(1894..2508)
                     /gene="plsY"
                     /locus_tag="KR314_000653"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBH0327056.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(2543..3862)
                     /gene="der"
                     /locus_tag="KR314_000654"
     CDS             complement(2543..3862)
                     /gene="der"
                     /locus_tag="KR314_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBH0327057.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(3896..5224)
                     /locus_tag="KR314_000655"
     CDS             complement(3896..5224)
                     /locus_tag="KR314_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBH0327058.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(5462..5662)
                     /locus_tag="KR314_000656"
     CDS             complement(5462..5662)
                     /locus_tag="KR314_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327059.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(5664..6596)
                     /locus_tag="KR314_000657"
     CDS             complement(5664..6596)
                     /locus_tag="KR314_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBH0327060.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNM
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(6641..7573)
                     /locus_tag="KR314_000658"
     CDS             complement(6641..7573)
                     /locus_tag="KR314_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBH0327061.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYDLSFFHCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNDVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(7628..8917)
                     /locus_tag="KR314_000659"
     CDS             complement(7628..8917)
                     /locus_tag="KR314_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0327062.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(8946..9638)
                     /locus_tag="KR314_000660"
     CDS             complement(8946..9638)
                     /locus_tag="KR314_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0327063.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(9653..10414)
                     /gene="pgeF"
                     /locus_tag="KR314_000661"
     CDS             complement(9653..10414)
                     /gene="pgeF"
                     /locus_tag="KR314_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBH0327064.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENITSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(10411..10872)
                     /gene="nrdR"
                     /locus_tag="KR314_000662"
     CDS             complement(10411..10872)
                     /gene="nrdR"
                     /locus_tag="KR314_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBH0327065.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIEEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(10894..11157)
                     /locus_tag="KR314_000663"
     CDS             complement(10894..11157)
                     /locus_tag="KR314_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBH0327066.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(11261..12034)
                     /gene="sigG"
                     /locus_tag="KR314_000664"
     CDS             complement(11261..12034)
                     /gene="sigG"
                     /locus_tag="KR314_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBH0327067.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(12126..12848)
                     /gene="sigE"
                     /locus_tag="KR314_000665"
     CDS             complement(12126..12848)
                     /gene="sigE"
                     /locus_tag="KR314_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBH0327068.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(12873..13745)
                     /locus_tag="KR314_000666"
     CDS             complement(12873..13745)
                     /locus_tag="KR314_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBH0327069.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGSAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(14036..15310)
                     /locus_tag="KR314_000667"
     CDS             complement(14036..15310)
                     /locus_tag="KR314_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBH0327070.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(15594..16751)
                     /gene="ftsZ"
                     /locus_tag="KR314_000668"
     CDS             complement(15594..16751)
                     /gene="ftsZ"
                     /locus_tag="KR314_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBH0327071.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(16941..17294)
                     /locus_tag="KR314_000669"
     CDS             complement(16941..17294)
                     /locus_tag="KR314_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBH0327072.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(17393..18067)
                     /locus_tag="KR314_000670"
     CDS             complement(17393..18067)
                     /locus_tag="KR314_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBH0327073.1"
                     /translation="MKKKIVILGAFILGIFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(18072..18770)
                     /locus_tag="KR314_000671"
     CDS             complement(18072..18770)
                     /locus_tag="KR314_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBH0327074.1"
                     /translation="MKKRFINKSIIIGSVVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKTEKENLQSEVNSVKKDIETYRGKNNKGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVFNTYMY
                     LKEDKLCMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(18793..19533)
                     /locus_tag="KR314_000672"
     CDS             complement(18793..19533)
                     /locus_tag="KR314_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBH0327075.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNINLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSVDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(19803..21029)
                     /gene="murG"
                     /locus_tag="KR314_000673"
     CDS             complement(19803..21029)
                     /gene="murG"
                     /locus_tag="KR314_000673"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBH0327076.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(21047..22177)
                     /gene="spoVE"
                     /locus_tag="KR314_000674"
     CDS             complement(21047..22177)
                     /gene="spoVE"
                     /locus_tag="KR314_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBH0327077.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(22229..23584)
                     /locus_tag="KR314_000675"
     CDS             complement(22229..23584)
                     /locus_tag="KR314_000675"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBH0327078.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEVVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(23603..24571)
                     /locus_tag="KR314_000676"
     CDS             complement(23603..24571)
                     /locus_tag="KR314_000676"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBH0327079.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(24612..25985)
                     /locus_tag="KR314_000677"
     CDS             complement(24612..25985)
                     /locus_tag="KR314_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBH0327080.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREASAQNAFVA
                     IVGENLDGHTFMKPAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDIYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(26152..28131)
                     /locus_tag="KR314_000678"
     CDS             complement(26152..28131)
                     /locus_tag="KR314_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBH0327081.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(28143..28490)
                     /locus_tag="KR314_000679"
     CDS             complement(28143..28490)
                     /locus_tag="KR314_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327082.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIAIFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(28976..29911)
                     /gene="rsmH"
                     /locus_tag="KR314_000680"
     CDS             complement(28976..29911)
                     /gene="rsmH"
                     /locus_tag="KR314_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBH0327083.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGKLVDIIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNKGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(29941..30687)
                     /locus_tag="KR314_000681"
     CDS             complement(29941..30687)
                     /locus_tag="KR314_000681"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBH0327084.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKDDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKHNISEE"
     gene            complement(31084..32370)
                     /locus_tag="KR314_000682"
     CDS             complement(31084..32370)
                     /locus_tag="KR314_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBH0327085.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(32385..33650)
                     /locus_tag="KR314_000683"
     CDS             complement(32385..33650)
                     /locus_tag="KR314_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBH0327086.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            33966..35063
                     /gene="ychF"
                     /locus_tag="KR314_000684"
     CDS             33966..35063
                     /gene="ychF"
                     /locus_tag="KR314_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBH0327087.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            complement(35542..37821)
                     /locus_tag="KR314_000685"
     CDS             complement(35542..37821)
                     /locus_tag="KR314_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0327088.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITEKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYIARSNGNIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFAQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(38092..39546)
                     /locus_tag="KR314_000686"
     CDS             complement(38092..39546)
                     /locus_tag="KR314_000686"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBH0327089.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYNSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            39710..>40151
                     /locus_tag="KR314_000687"
     CDS             39710..>40151
                     /locus_tag="KR314_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBH0327090.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDATSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILR"
BASE COUNT        12617 a         7248 c         4169 g        16117 t
ORIGIN      
        1 tatataaact atattgatta tttgtaggtg aataaaatga caaataaaga attacaaaag
       61 aaaaggatat tgatgtattt catagaagct gctcaaaata ttatggaaaa cgaaggtata
      121 gaaaatatta cacttcgtaa ggttgcagat atggctggat ataatagttc aactctatat
      181 aaatacttta aaaatttaga ccatttaata tcatttgcat ctataaaata ttttaaagat
      241 tataacttga atatttcaag atgtatagaa aatgtcaatg atgaatataa gaaatatata
      301 attatgtgga aattattttg taagcattct tttgacaatg ctcaagcttt ttatcaggta
      361 ttttttaact tgtctagtga tgaacttagc tatataacaa aacaatatta tgatatgttt
      421 ccagaagatt taggtattca tgatagtgac atatctttaa tgattacagg agaaagtata
      481 aaggaaagaa ataagatact attaaacaat cttgtagata ctggatatat accagctaaa
      541 gatttagaca taatcaatga tataattatt tctttttatc aaaatctatt attcttaaaa
      601 aagaacacag gaaaaaattc tggtgatgat gaattaacta tgaaaatgat ttcttctatt
      661 gaatacataa taaaacatgc taaataaaat taaaatatgg ctgtctcaaa atagagataa
      721 gcctttttta ttgttgaaat aataaaaata tatttaattt catataaaaa aaagtgcctc
      781 gtaaactaaa aagttcattt tgagacaccc ctaaaacaaa tttattattt attatacaat
      841 agtttatata tcatcaccta aaactacttc ttccatttca tgctttttac ttctcatcat
      901 tagttctatt ccaacttctt taggatttga acctttattt attacagaat atatagcatt
      961 agttataggc atatctatat ctaatttttc agcaacatca tgtgctactt cagtagcagt
     1021 tatgccttct acaaccatct ttacttcttt taaagtatct tctaaactca tacctttacc
     1081 tatgagtata ccagctcttc tatttctact atgcatactt gtacaagtaa ctattaaatc
     1141 tccaatacct gaaagacctg caaaggtaga catattagct cccatagcaa ttccaagtct
     1201 gctcatttca ctaatacctc ttgtcataag ggcagctttt gcattatcac cataccctaa
     1261 tccgtcacat atcccagctc caaatgctat aatatttttt aatgctcctc caagttctac
     1321 tccaactatg tctggattcg tataaactct aagtttaggg ctcataaata aatcttgtat
     1381 catctgtgct atttttaaat ctttagaagc aactactacg gtagtaggaa tatcccttgc
     1441 tacttcttca gcatgagatg gcccagatag tattacatat ggattttgtg gtaattcttc
     1501 ttcacagact tgagataatc taagcccagt ccctttttca agacctttag ctacattaac
     1561 aataacttgt ccttctttta taaatggttt tattttttgg caagtacttc ttacagcctg
     1621 tgatggaact gctaaaacta ttaccttaca atcttttata gttttttcta tatcagtact
     1681 tatagttata ttgtttggaa ataatactcc tggtaaatag tctgtatttt ctttagttct
     1741 gtttattttt ttagcttgtt cctcttttcg agtccacatg ctcacatcat tacctttttt
     1801 tgcaagccct aaagctaatg cacttcccca gcttccagta cctaatacac atactttctc
     1861 catgtttaac accacctgag tataatatat ttactattct ttcttttgac caatttttct
     1921 ctctgtccca tttaataatc gctttatatt tgctctgtga ttatatatta ctgatgcagt
     1981 taagaacaga gctactatta aaccagcttt attttttacc ataatcataa atattggtga
     2041 acatcctatt cctacaacag aacccaatga aacatacttt gttatagcta ccaccaatat
     2101 aaatactgct agacacatta gtgttattac tggatttaca gctagcattg cacctagtga
     2161 agttgcaaca cctttacctc ctctaaatcc taaaactgct ggccaattat gacctgctac
     2221 aacacatata actgccagat atccagctaa aagagtatct attccaacca atcttgctgc
     2281 aaattctgat attaaaacag ccattacacc tttaagaaca tcacctaaaa atgtcattgc
     2341 tcctgctctt tttccaagtg tcctaagtac atttgtcgaa cctgcattac cagaaccttg
     2401 agtccttata tcaaccccag caattcgctt tgcaactatg tatgaggtag atatatttcc
     2461 taaaagatat gcaactacag caataattat ataactaaat atctccactg aatttccccc
     2521 ctttagttat attttaagat atttattttt tatttttttg tctatactca aactttatgg
     2581 aagttccttc aaatccaaaa ttttctctta tcttattctc aaggtatctt tgatatgaga
     2641 agtgagttaa atccttatca tttataaaca atgtaatttt aggaggtcta atgtcagttt
     2701 gagtcccata gtaaattttt agtcttctac ctttgtcaga tggtggttga tttaacatta
     2761 cagcttctcc aattacttca ttcagtgctg aagttgatat tctcttagaa tgttcatttg
     2821 acacatattc aactgtatct agtatcttgt tcattctttg attagttttt gcagaaacaa
     2881 atacgattgg agcatacatc ataaatggaa atttttcttt tatatctttt gtgtaattac
     2941 tcatagtctt attatctttc tctattaaat cccatttatt gattacaaat atgcatcctt
     3001 ttccttcatc atgtgctata cctgcaacct ttgtatcttg ttctgttaca ccttctaagg
     3061 catctattac tatcaatact acatctgctc tatctacagc actcatagct cttattacac
     3121 tgtatttttc tattgtctca tatattttac tttttcttct aagccctgct gtatctatta
     3181 gaagaaattt ctgtccattt ttttcaaaat aagtatcaat tgcgtctcta gtagttcctg
     3241 caattggact tacaattact ctttcttctc caagtatctt attgagtata gaactttttc
     3301 ctgcatttgg tttacctgtt atagctactc ttattatatc ttcttcatac tctgtatcta
     3361 atcctgctgg aaaattttca actatctcat ctaataaatc tccaaatccc atactattgg
     3421 ctcctgaaac tgcaaaaggc gttccaaatc ccaactcata aaaatcatat atattatcaa
     3481 attgactttg actatcaatc ttattaacca ctaagattac tggttttttt gtctttctaa
     3541 gcaattgtgc aatttcttta tcagcagctg ttattccagc tttaccatca actataaaaa
     3601 gtattacatg tgacatgtcc attgcaagca tggcttgatt tctcatttga gataatatta
     3661 tatcttcgct atcaggttca attcctcctg tatccactaa tgtaaaatat ttatctaacc
     3721 actctacttc agcaaatatt ctatctcttg taactccagg tgtattttca actattgata
     3781 tcctttttcc tgcaaattta ttaaatattg tagacttacc aacgtttggt ctcccaacta
     3841 cagctacaac tggcctactt atactcattt tatttctcct tccatctttt gttttctatt
     3901 ttaaaatttt atccacaaag tcttttcctt cattcaaaca agggataatt tttattctca
     3961 taagttcttc taattgattt aattcaatat tatctaagaa aatttcttca tctgctttta
     4021 gcatacttct tgttatataa agagcttctc ctaattcttt atcttcaagt tgttctttta
     4081 aatccttagc tgttaaaaga cctgatacag ttattgtctc tccaaaaaac ttgtttttga
     4141 tttcatatac atttacttga agatttttaa acttgtccat catagcatct gccatactct
     4201 gtataaattc atatgcagaa tgaccagtag ctattgatac tttcttctct ctttttagaa
     4261 tattctcagg caatatattt agatactctt caatctctgt ttttaactta cgtatcatac
     4321 ctacaccatc ttcaaactgt aaaaaacctt catattcttc atatccaggc aattcactat
     4381 tagatagtat ataaaattca tctgataaaa atgcaaatcg agtacctaat ttttcaagat
     4441 atttctgttg tatttgatgt atttgctcta tagttttact tgcgctctta tcattaaata
     4501 tatttaattc tactagattt tctctatgct ttgtaatccc aactggaact atcgcaacac
     4561 tgtttacata tggataaagt tgagctagtt cttttattgt tctatccaat tcctttccat
     4621 catttatacc tggacataaa acaatttgac agttcatttc tatatgagca tctgccaatc
     4681 tcttcattat accatataat tttcctgcaa atttattgct tatcattttt tgtcttaact
     4741 ctggattagt tgtatgcaca gatatattta taggacttat tctatatttt ataatattat
     4801 ttatatcttc ttcactcata tttgtaagtg tgacaaaatt tccttgtaaa aaagataatc
     4861 tcgaatcatc atctttaaaa taaagagttt ctctcatacc ttctggcaat tggtctataa
     4921 aacaaaacat acacttattt ctacagcttt tagcttggtc aattatcgga tttgtaaatt
     4981 caacacccaa atcttcatca tagtcttttt ctatctcgta tatgtagact tcgccatctt
     5041 ttttttgtat ttctacatct aaatattcat cacttagtaa gaatctatac tctattatat
     5101 catgtattgg ctgttcattc acagatataa gtaaatctcc tacctctata cctatttctt
     5161 ctgcaatact atctttataa accttactta taatattatt tacttttttt acatttactt
     5221 ccaatatttt caccactctt ttgtattcat caatgcttat tataatatat tttaatatgc
     5281 aatttattta tttattatat tttatgatag catttttaag catttcatgt cataataaca
     5341 cattttaatt ctaatagtca atataagtaa atagctataa aatagattag aacaaataat
     5401 attcaaattt aagtttctta aaaatttgaa tattatttgt tcttaaaaaa attttataaa
     5461 tttaatgttt tactatgatt aacgtcccat ttgtcatatc atgaaccatt ccacctacac
     5521 actttgacat caacaatgcc ttattttgta agtctttctc atcatttgca taaaatattg
     5581 gacaacctcc accatttttc atattcttat caagtgtaac tatagctagg gtatattcat
     5641 ttaaacctat atccattccc atcttatgca cttcctttct ttcctatttt ataatttttt
     5701 agtgataagt tttttccttt agagcttgaa agtattgggc atgactttac agcttccatt
     5761 actttttcca tattcttatc tactggtaaa taagcaatca ttatactttc atcctcttta
     5821 tttcttctag gtaatggtac gaatgaaggt tctccataat ctccatataa acctaacctt
     5881 gaatatatat tgtatactat tgcttgtctt tgaccggagt caaataatat tcctgcatta
     5941 gcataatcct tatttttagg aattatttgt attcctacac ctctattaat atatacttct
     6001 ctatcacttt ttagacctat atttgttata ccttttaaat ctcccacctt tagtatagat
     6061 tcgtccacaa aactaatttc agcaggcatt acatcagcta tgtctccaat actttttcta
     6121 gtaagaagtt tttttaatat aaaggctaac actaatccac aaattgaact tattaccaaa
     6181 ctcatccctt cacttatttt cgtttctgca agtattacat aatacaatcc tattgttaaa
     6241 aaagatgtaa ctatacacat ataatttctt acttcatagg ttctagctat ctcttctatg
     6301 aatgcagcac ctctctttac aagttgaact tcctctaaat tttgcaatgt atttcttcta
     6361 tttgctctca cttgtctaaa ttgttctgct gctagagata aaaatgttat ggatgtataa
     6421 gaacgattta taagagctgg aaccaataat gctcctaatg aagaagcgac gaatgctaat
     6481 ataagttgag atagtaaaac atttggttgc gttggatact gtttttggtc taaattcaac
     6541 attataattc tagatgttat accaataata acagcaacaa aaaatactct atccattaaa
     6601 tcactcctaa aatatttaat ttgaaatttt acatgtaaat ctacaaagtc tttctagaaa
     6661 aatcactctg ctttcctttt gatgtttcta tgataggtgt acttttcact acatctatca
     6721 tgacatctat atctttgagt attggcatat atggtataac cacagtactt ttttctaaat
     6781 tggtcttaga tatagcaaca atatcaacct catctacatc tctatctatc cccatatgaa
     6841 taaatagatt atgtattata gcttgtcttt gccctatatc atttactatt ccaaatgcac
     6901 ctaaatctct tggtataact tctatggcca gacattcatt tctatacttt tctcttgtac
     6961 tttctagacc tatattagtt attattacat catttaccat taaaataggt ccttcaaaac
     7021 ttatttttgc aggaactata tctgctatat ctgctatact gtttcttctt aaaaatcttc
     7081 taaaaatcaa gccaacaata gctcctgaca caatagctaa aattgtgcaa tgaaaaaagc
     7141 taaggtcata ctttcttgca aaaacgatat aaactatcga agatactagt gcagaaaaaa
     7201 gacttatata acttctagat tcatacgtag atgctatttc ttcaacataa gcatctcctt
     7261 ttggtactaa ttcttcatta tcaatattct ttagtgttat tctttcttgt tccgcaagac
     7321 cttgaaactg ttgtattgct actgcaaaaa atgtcaaggc tgcaaactcc ttatctataa
     7381 gtgcaggtaa tgctatagca cctaacgaag ctgaaagccc agatataatt atctgttcta
     7441 aataatcttg tggtcttgat gggtattgtt tgtctgtaac tcttaaaact aatcctctac
     7501 ataatattcc tacaactaat gcaactataa aagagtgtct aaataaattt gcattcaata
     7561 aactttcttc cattttctca gctccttctc ttatatagtt tgtgcaaaaa acaaaaaaaa
     7621 ttcccttcta tttaaggaaa tttttttgta atattgtaac tataaattcg ctaccaatgc
     7681 caagttcact ttttactttt atacatccat ttagtgattt gactatatgc tttgttatag
     7741 ccaatcctaa acccgttcct cctacatccc tacttcttgc tttatctacc ctataaaatc
     7801 gttcaaatat tcgttctaca tcttcttttg gtatccctac accattgtct ataactttaa
     7861 ttacgatttt ttcttcatct tttaacacta caacttgtac aatcccatta tcttgggtat
     7921 actttatagc attatcaact aggtttaaga acacttgctt tatatagtct ttattggata
     7981 gtatacttat tgatttatca tcacatttat ataaaacttc tatatttttt tcactagctg
     8041 aatgttgagt tatttcatat acctctttaa atacatcata tactacaaca ttttccataa
     8101 gaagagtatc tttattttct acaaatgata acagcaatat atcatcaatc aaccttttta
     8161 atctatctga ttcgctttct attatttcta agaatctatt tctagtattc acatctatat
     8221 tttcatttga ctttagtgtt tcaacaaacc cacttataga tgtaagtggt gttttaagtt
     8281 catgacttac atttgcaaca aaatcacttc tcatattttc aagtttaact tttttagtaa
     8341 tatcttctat atttactatc gaacctatta ttacgttgtt agtattctga agataaactg
     8401 ggtctacctt tatcttatat acgatatcat ctttagtagt aatattagca gtttcattat
     8461 ctttagaacc aataaattga agtatttgtt ttaatatttc ttcttctttt attacaacat
     8521 taatattatg accttcaact aattgatttg gttcactctt tatcatttct tttgcttcat
     8581 catttatcag aagtatattt ccatttacat ctatagccaa aattccatga gatatactct
     8641 ttaatataga tcttaactgt aaatgtttat actgtacttc ttctaaggtt ttatccatat
     8701 tctttatcat atgattaaaa tttctagcta attcaccaag ttctcctttg gctgatatgt
     8761 taagcctagc atgatattca ttattactta ctttttttga gacgtatata aactctttta
     8821 aatatttcct taatgttatt gtatatctta cagctaatat aacaatggct atggacaaca
     8881 taacaataaa aaaatatata ctatttaaat cttccacaag ataacactcc taaaatataa
     8941 cctttttaat ctattttata ccctacacct cttatagtct ctatatattt ttccgattta
     9001 tcttcatctt ctattttttt tcttaaatat cttatatgaa catctactgt tctagtttct
     9061 ccatagtatt cataccccca tattttatcc aaaagataat ttcttgaaag tacttttcct
     9121 ttattttgaa ttaataattt taacaattca aattccttaa gagttaactc tatcttttca
     9181 gagcctttgg taacttcatg ttttgataaa tctagtttta aattgcctgt agtaagtata
     9241 ttgttttctt cacctaagct agtcaaatta tatctcctta aaacagaact aatcctagct
     9301 aacaactctt ttatactaaa tggcttagtt atatagtcat cagcacctat ttcaagccct
     9361 tcaaccttgt cattttccat atttttagca gtaagcatta taacaggaat attttttaag
     9421 tccttatcac ttcttatttt tctcaaaact tctattccac ttatatttgg taacatccaa
     9481 tctaaaagta ttaaatctgg tttaatttcc ttagccttta taaatccgtc aaatccatca
     9541 tatgaataac taacttcgta atttgacacc tccaaattga attttaatag ctcaactata
     9601 tgcatttcat cgtctataac aagcacttta gtattcatta aaaagctcct ctctatttta
     9661 tctgtagtac cgttcctatt cgttctgatg ccttattatg ttttctataa gagtggaatt
     9721 tatctgctct acaactagtg cataaattta aatttattat attttctttt ttaactccac
     9781 tacatctaag catatactca ttaattttcc ataaatctaa atagtaactt ccctctttta
     9841 ttatatagaa cttttcatca ttatttgtaa gaattgtgtt aaatttttct actaaatctt
     9901 cagacacttc atagcaacat ggtccaattg atggcccaat tatacatact aaatcttcag
     9961 gaactgtttt atagcattca gacattaatt ttattgtttt atgacctata ttactaaaag
    10021 tccctctcca acctgcatga gccagtccta tggctttatt tttagcatct ataattgata
    10081 taggtacaca gtctgctgta aacagtaaaa gtggtacttt ttccaagttt gttataagtg
    10141 catctccatc tcttcttttc cctatagtat ctttattgac tatattaact atatctgaat
    10201 gtatttgaga attactagtt atattttcaa atgtcaaatc acattcttta catactttta
    10261 acatatcttc tctatttttt gcatctatat tcttacatgt tataactaga tttacagaat
    10321 ttaatttttc atcaagttct tcacaattac cacttaaatt aacatactct ttaatatctt
    10381 gtacattttt tatatttata taatctttca tcatttgtct cctttttcta tcagtatact
    10441 tttaagttca tttacaaaag tatttatatc tttaaattgt ctatacacag aagcaaatct
    10501 tacataggca acctcatcta actctttcaa tttatccatt accatctcac cgattttttt
    10561 ggtttcaatt tcttctataa aacatttatt tatttcattt tcaatatgta caacaatact
    10621 ttcaatctct tcaatagaaa caggtctttt ttcacatgct tttagcaatc catattttat
    10681 cttttctcta tcaaaatatt ctctactatt atccttttta atgaccatta aaggagttgt
    10741 ttctattttc tcatacgtag taaacctctt tttacatgat tcacactcac gtcttcttct
    10801 gatagatttt aaatctgtat gtctagaatc tattaccttt gattctttat aattacaata
    10861 aggacattgc atctatattc ctcctttgtt taactatatc tcaaactcat ctaagcttaa
    10921 ttctgaacct atatttacta ttattgtatc acaacctatt ttaagtatat cattccaaaa
    10981 tataacttct tcatcccctc ctcttgaaaa aaaacttcta cttccatctc caaaaatggt
    11041 aaatgagact accttacctt cgtgaatatc catgtctatg tctattataa accccatcct
    11101 ttttccattt tttacattta taatttcttt ttccattata tcagaaactc taatcatatt
    11161 gtactcaccc tttctttaaa aataaatatg tatcgaagta aaaaaaaatg acctatatag
    11221 gtcatttaaa ttaaaataat gtttacagtc catggattac ttatacatat tttctcatat
    11281 tttttaaagc atttttttca attcttgata cttgtgcttg agatataccg atttcatcag
    11341 caacttctat ctgagttctt cctttgtaaa accttaaatc taataccaac ttttctctac
    11401 tatttaattt cttaatagct tctttaagtg atatttcttg caaccaattt tcatcagtat
    11461 cttttttatc ttgaacctga tccataacaa atattgcatc tccattgtct tgatatactg
    11521 ggtcaaacag agatattggg tcttgtatgg catcaagtgc cattacaact gattctactt
    11581 ctaattctaa ttcctttgct atttctgata ctgtaggttc tttagaattt gttctaatta
    11641 atctttctct aacttgtagt gccttatatg ctatatcttt taatgaccta cttactctaa
    11701 ttgggttatt gtctcttaga tatcttctaa tttctcctat aatcatagga acagcataag
    11761 ttgaaaacct tacattttgg cttaaatcaa agttatctat agcttttata agaccaatac
    11821 aacctatttg aaataaatcg tctatatttt ctcctctgtt gttaaacttt tttattacac
    11881 tcaagactaa tcttaaattt cctcttacaa actgttgtct ggcttcttca tcaccatttt
    11941 ttatttgtaa taaaagttcc ttcatttgct tatttttaag aactggaagt tctgatgtat
    12001 taacaccaca tatttcaacc ttattaactt gcatatattt tcagtcctct ctcaaaagta
    12061 ttcatctatt gaaattattt acatttccaa tttttttata ctttttaaga agaattaatt
    12121 taacattata caaatttttt catttctttt tgcaaccttg agattatttt cttttcaagt
    12181 ctagatatgt aggactgtga tattccaagc attccagcaa cttctttttg tgttttttca
    12241 atacctttat caataagacc aaatcttaat tccataattt gcttttctct gtcacttaat
    12301 ctgtcaagtg ccataaccaa caaatctcta tctatttctt cttcaattat tttgtatatt
    12361 tcatcatttt ctgtacccaa tacatcagat aataaaagtt catttccatc taaatctata
    12421 tttaaaggtt catcaaatga tacttctgtt ttctttttat tattttttct taagtacatt
    12481 aatatttcat tttctataca tctcgaagca taagttgcca attttatatt tttattcagt
    12541 ttaaaagtat taactgcttt tatcaaacct atagttccta ttgaaatcaa atcttctaca
    12601 tctatacctg tattttcaaa ttttctcgat atgtagacta ctaatctaag atttctttct
    12661 attaaaattg actttacact ttcatctgtt tctagctttt gtaaaagctc catttcttct
    12721 tctggtttca atggaggagg aagtatattt gcccctccca tatagtatat tccctttggc
    12781 tttattattt ttaatttaat acctaacata gtaataaagc ttattattct ttctttcaat
    12841 cgtaacattc cttgtccccc ttaaattttc acctaaaatc caggcaatat atttggattc
    12901 attattgctc catattctct atcattaaaa tttgaaatac caagtattat atttcctatc
    12961 ttttgctcat ctacttctat atagtcagct tttaagccta ttattatgct tgtcttatta
    13021 cttccagcat gtttataagg tattattcta actctacctg aagttttatc atctaaactt
    13081 cctataattt cttgtgcttt cttcatatct atatcttcat aattaatatt taatagtttt
    13141 tctggaataa atccttctaa cacactagac ttaactatta ttacatcact ttgacttatt
    13201 gggtctttta acaaattacc actatctact aaagctttac agcaaaaact tttttctagt
    13261 aagtttatat ttatagtctt tgtaagctcc ttaatgtact taagcatttt caaatcttta
    13321 taaatgtatt ttaataattc acaactcaca taagtacatg caattaagaa agatattttt
    13381 aagtgactta ttcctgtaaa ataaattata aaataagtgc ttccagaaat aaaaatgttt
    13441 actagataaa aaactaacgc tacatgaaca taatcttttt tgctcttgct tctaaatgaa
    13501 attaaagtta ttatgctcat gattattatt ttaaatggta cagtaaatag tatattcatt
    13561 gatggataca aataagcaac agaataggca cttcctaaac tagcccccaa taacttattt
    13621 tttttaggat tatatctttt agtaagaata gaagtacaac ttataataat ataatttatt
    13681 actaggtttt ctataacata gtactcaata tacaccatgt atcccccctt aaaaccttta
    13741 aacatattat aagatagtat gtacaaaatt ttatgtcact tttgatggtc gaatccaaaa
    13801 caaattctat ttaaaaagaa caatgatata tgtcttatac tttatgtcgt caaaagatat
    13861 ttttttaatg ccaaacagat agactttttt gtagaatcca atatatctag gtagaagtta
    13921 ttcgctttac ttttggtata tttataaaaa aaagactaca taatttaata atatatcaag
    13981 taaatctttt acttatataa aatcaaatta tatagtctgt aaattcaaat ataaattatt
    14041 gtgctagtaa tccatttgca tattttgcag attcatttag tgcttgttct ggagttatgt
    14101 ctccagctat catcatttga agttgtttcc ataaatattc atatatctct actccatgag
    14161 gtccaactgt caatggtcta tacatatgtt tatctcttgt caccatttct tcaaatcttg
    14221 ggtctccttg gtatttttca cctttagcaa ctggagctct aggtatttct ttgtggaaag
    14281 cttccattga ttctttgctt aacatatact caagaagttt aaatccaagt tctttatttt
    14341 tacatgctga cattaatgtt aaatcatcta ctgaagcaaa acttccaact gtcttatctt
    14401 ctaatgatgt tacatatccc cagtttaaat ccgggaatga tttatcaaat gtacttgtag
    14461 cagcagagga tagcattata gtaaatgcag cttttcctgg tccaaataca ctttcaatca
    14521 tttcattact atcttttgat aaagcatctt ctggaatata ctttttcata tcatttataa
    14581 agtttgcagc tttcaagcct tctgcattat taaatttaac tgattttaaa tcatcattat
    14641 atatatctcc accagcttgc cataaatacg gataccagtt ccagtttaag cttccaaata
    14701 ctttagaacc ccaaccttgt gcaagacccc actggtcgat ttttccatct ccatcagtat
    14761 cttttgttgc tttttcacag attcttttaa aatcatccca tgtttttgga ggtttttctc
    14821 ctaatttttc tagtatatct ttattgtagt acaatacgcc tggattagca gcttctatag
    14881 gtaaaccata aataccaccc atcatttttg catcatctaa gtatagataa ttatctgaag
    14941 ttcctgattt ttctaaataa ggtgttaaat cttctactgc acccatttct ataaattgag
    15001 ggaacatctc tgcatacatg tatcctatat ctggaccttc acctgcactt attgctgttg
    15061 catatttttc tgcgtagtta tcccatggta ctatctgaaa ttcaatttca cagttatttt
    15121 ctttttcaaa tttttcaaat atcggcttcc atgcatctgc atcatggtct gttaatggtg
    15181 cagaccatac aactaatttt tctttaccct ctttgccttt agaatcttct ttttttgaac
    15241 atcctgcaag aattgatact gatagaatcc ctaccataac taaagaaaga acctttttta
    15301 gtttaatcat gcttaatccc ctttcagact ttaatttgtc tttttcctca aatgtttaca
    15361 tttaattcag atttaatatt aacaaataag actactcttg tctaatcata aaaaactata
    15421 ctaactccta taactatatt ttttttctat acctattaga tttaattcat actaaaaact
    15481 tactttttaa attttatcaa tatttaaaaa tatttttaag tctagccaca aactaactat
    15541 ctaaattgaa caaaataaaa agaagtctat tgacttcttt ataagcttac atattatctt
    15601 cttcttctta ggaatgtagg tatctccatg ctctcatcat catcttcttc aattatactt
    15661 gcctttttag gaggctctat tttttcctca gcaattactt cttcttcaat ttcttttaca
    15721 gcttgtttta ctgttgtatt taatgaagaa cgtatgcttg atttaggttt agtatctaaa
    15781 tctaaggcac cattttgtaa tccttcaaat cctgttgcga ttactgtaat cattatttca
    15841 tcacctaaat cttcttttat agaagctcca aatataacat ttgcttctgg gtcacaggat
    15901 tccataacta aagttgatgc ctcattaact tcaaataatc ctaagttagg accacctgta
    15961 acattaagaa gtactccttt agctccttgt attgatgttt ctagaagtgg agattgaata
    16021 gcttgtcttg cagcatcaat tgctctagtt tcaccactag cactacctat acccatatga
    16081 gcaagtccct tgtctttcat tatagtagta acatctgcaa agtccaagtt tattaaccct
    16141 tcaactgcta taaggtctga aattgattgt ataccttgtt ttaatacatc atctgcaact
    16201 gcaaaagcat ctaacattga tgtatttttt tgtactatct gtaaaagtct atcatttggt
    16261 atcgttataa gagtatctac ttttgattta agttctgcta ttccaccttc agcgttcttc
    16321 attcttatct taccttcaaa tgcaaaaggt ttggtaacta cacctactgt aagtatcccc
    16381 atttcttttg ccaagcctgc aaccactgga gcagctcctg taccagtacc accgcccatt
    16441 ccggctgtta caaagaccat atcagctcct tgaagtaact ttactatttc atctttactc
    16501 tcctcagcag ctcttttccc tacttctgga tttgctcctg ctccaagtcc tctagttaat
    16561 ttttctccta tttgaacttt atattcagct tttgatgtat ataaagcttg tttatctgta
    16621 tttactgata taaattcaac gccttttagt tgagcttcta ccattctatt gacagcattg
    16681 tttccaccgc cacctacacc tattacttta atttgtgcac attcttctaa ttctacgtca
    16741 aagtttagca tcataaaccc ccttctctca tgattcaaag ttatcgtatg tattaattat
    16801 atcattttct tcttttattt tgttaacctt taagtaacat attccacaaa aacgtaaatt
    16861 ttccttttta aatttcttac ttatattgtc aaatattact ctttaagtat aaaataattt
    16921 acgtatatat taaatgctct ttaattttta cgttttttgt tcataatatt actaataata
    16981 tatcgtctaa ttatagataa gttattaaac aatctcatac caaaaactag tacagcaaca
    17041 tagtaaagag aaactcctaa cttttctcct atataagtca ataatatagc caatatagca
    17101 ttacctatga aaccagtaat aaaaattaca ttatcatagt tatcttcaaa attggatttt
    17161 atcgctccaa aaatagaatc cattattgca agaatagcta ctgacatata tatggaataa
    17221 tccatagaat atgtaaatgg aatatagtaa cctactacta caccaaatgc taatcctcct
    17281 aaaacccaca acataaatct tcacctcttt attttacttt gaaacacaac tacattacaa
    17341 tcctttttat taattttttt tactttttct ttattttttt gtatttttcg ttttattttg
    17401 caaataaatt cactttattc ccattatttt cctgaacttt tatattaatt ccatatatat
    17461 ccttaagaga tttagcatat gattctggag agtttatagc tgcactcaat aaatctatat
    17521 ctcctatagc ttttattata aatggttgtc catatgtagt atcatttact gtaatagtag
    17581 ctcctgaaca ttttatctta cttgttgcta gtactctttc cccatttata gaaattgctc
    17641 ttgctccagc cttttttaaa tcatttaaaa ctctcaaaat atctatatca tgtataatca
    17701 aatcatttgg attctgtccg tcttttaact ctctctcact atccctcata gtaatagtta
    17761 tgcctggccc tgtaacagtt gaataaccac ttaattcttt aacttcttca agctcttttt
    17821 gcataagtgt ttggatagat tcatttccat tcttttttgc ttctttatat tcattcaaag
    17881 tttttttgtt gtttttaaga gtttctttta aattttttat ttcttttttt gtattttcta
    17941 cctcactttc aatactttca gtttgaccta atgttatgta tactctattt gggtctagtg
    18001 attttatata agttgatatg aaaatgccta atatgaatgc tcctaatata actattttct
    18061 ttttcatact attatttccc ctctgctttg tttatatcac tatgaccatt tatttctata
    18121 ttgtcttctt tggtcacttt tactttggca ttataatatg tttctatttc ccagacaata
    18181 ccatacttta tattaagtgc cgaagccaat gtatcagaat caccaattgc ttttattata
    18241 attggttctt ttatgacagt gtcattcata caaagcttgt cttcttttaa atacatatat
    18301 gtattaaaaa ctattctctg gtcgtttata gatatggcac ttgcttgtgc tgaatttaat
    18361 ttatttatca ttgacaataa taaatcataa ttatatagta tactatcatt tttatcagag
    18421 tttcctaagt cttttgtaga atttatctca atttttatgc ctgttccttc aacatctgta
    18481 tatccagcta atgtttcata tttttctatt tctgacttaa gtatgtcttg ttctccttta
    18541 ttgtttttgc ctctataagt ctctatatct ttcttcacac tattaacttc tgattgtaga
    18601 ttctcttttt ctgtttttaa agatttaagt tcttctgtaa gttgctctcc tcttttaaaa
    18661 gttgtcatcc ctctattttc tatattaata attttaagtt gtaaactaac aagaacaccc
    18721 aatacaacac ttcctatgat tatactttta tttataaatc tttttttcaa tatatcactt
    18781 ccttacaatt gtctatcctt ctggactata cactgccaac tttccatagt taagatttat
    18841 agtccctcca ttagtatttt tattttgcag gtcaatcaga attttactta ccctagatat
    18901 attataatct aagttatcat cattagaaag taagattttt atattacttc ttgtaagcat
    18961 ttctatattg ctttcttttt ttaagtttac ataatttatt tttctataaa ttccttcttc
    19021 ttttaaataa tttaaagttt taaatacatt ctccttagtt ttataatttt taaattttat
    19081 agatttatta tcatcaacgc tataatctac atctactatc aagtcttttt tactttcatt
    19141 agaacctctt agttcttcca ataaatttcc ttttttatct atataacaat aattatcttc
    19201 atcttttaat actgcaaata tttctttttc ttttaaactt ataattatct tattgggaag
    19261 ttttcttttt atttccacat tttctatata tggatttttt ataagtttat ttttcatatc
    19321 tttaaaattg tatgcaaaaa tattttcatt tagatttata tttagttctt tcattatatt
    19381 tgatttggtc accctcttat ttccaattac atctattttc tttacatcaa ataaatcact
    19441 ttttacaatt atacatatac aaaaagataa cataagtata aaaactaaaa ctatcatgac
    19501 attattcgta tttaactttc ttcttttttt caaatataat caaccccttt ggttaataaa
    19561 tacaccttta gagcttgttt ttcctacaaa aaaattataa tatttaaagt caaatttttg
    19621 cattttatta cataacatcc aaataaggta ttaaatttta taaataaatc aatatttcat
    19681 caaaataaac atagtatact acatactatt tatatattta aatctgtata catacaaata
    19741 aacccctaat ttataataat ttagaaaatc taatatatta taaattaggg gtaaatattt
    19801 acttatcttt tctttattcc tattacctta gcttgacctt ctatacttgg agtcgtttct
    19861 ttttttattt cttttacttc cttaataact ttttcctttt ttgttttttt agaagttgac
    19921 ttttgtgttg aattgtatac tttcattatc tcatcatata taaggtctat ggcttcaggc
    19981 ttacctatag ttttacttgc atttgccata tcaactaaaa gttctctatc tcctaaaagt
    20041 ttaaatactg ctgtatttag gctctctgga gttagatttt tttctaaaat agcaattcct
    20101 gccccttgtt tttctatact cttggcatta tactcttgat gattttcagc agtataagct
    20161 ttaggtatta ttatagatgg ctttccaaga gccgttatct cagctaatga aattgctcct
    20221 gcgctaccta taaccaaatc acttgctgca agtgcatttg ccatatcttc taagtaaggc
    20281 acaacttttt ggtatggttt aagatttata tcgctaatac ttcccataaa ttcatcataa
    20341 tagcttttgc ctgtagcaaa tataaaagct atatcttcat ttaccatatt ctttattaca
    20401 agtctcattg catcatttat ttttcttgaa cctccactac caccatagca aagtaccatt
    20461 cttttttcgt cacttattga tagattcttt cttgcaatat tttttcttga taataaaatt
    20521 tcttttctaa ctggatttcc tgtaaaaact aatttatctt ctgctgcctc tggaaatctt
    20581 ttatgcgaat cttcaaaact tgtaagtacc tttgtaactg ttttagataa aatcttatta
    20641 gttacaccag gaaaagaatt ttgctcatgt attatggctg gtattttccc catagatgca
    20701 ttaaataaaa caggtccact aacatatcca ccagttccta taactatatc tggtttaaat
    20761 ttcttaacaa tctttcttga ttgctccaaa cctttaaaaa gcttaaaaac tctttttaca
    20821 ttatcgaaat ctatttttcg cttaaaacct tgaactgtta cagtctttaa ttcaaatcca
    20881 tacttaggaa ctatttctga ctctattccc ttttccgtcc caacaaaaat tatctcagcg
    20941 tctggatgct catctcttat tttgtttgca atagctatag ctggatacac gtgccctcca
    21001 gttccaccgc ctgataataa aactttcatc aaatcatcat ctcctgttaa ttaattttga
    21061 catgctttga gatgtttaac actattccta cagctcccat aaagatggtg agtgaagttc
    21121 ctccataact tataaatggt agtgctacac ctgtagctgg cattgatgat gtcgcaactg
    21181 ctatatttaa agctgcttgt attccaattt gtgcacctat acctataaca accatacaag
    21241 caaatacatt agaagtcttc aaagctattc ttacacatct gtagactaaa actacaaata
    21301 gcataataac tattatacaa ccaatcaatc ctaactcttc tcctattata gcaaatataa
    21361 agtcattttg tggctctggt atataaaaat acttttgttt actctttcct aagcctagac
    21421 caaacaatcc acctgaacct agtgcatata gaccttgtat tacctgatac cccttaccaa
    21481 gagggtcttg aaaaggatct aaaaatgatg taacacgact aagtctatag ggttcagcaa
    21541 ttactaatgc agcaaataaa gcagccccac tccctatcat agcaaataca attttcatat
    21601 ccattccagc aacaaatatc attacaaaag taactagaat tacagtacct gctgttgaca
    21661 aatttggctg aagtattatc aacgcaaaaa atatccctgg aactacgaga agtggtacaa
    21721 caccttttgt caaagatttt attttatcat aatttttttc aattaatttt gcagttaata
    21781 ttattgtagc aaactttgcg atttctgcag gttgaaatgt taaagctcct attcccaacc
    21841 atctttttgc tccgtttgct tctatcccta aaggggtcaa aactaacaat aataatacta
    21901 ctgctatagc tccaattgct gtagcatttt tcttccaaaa actataatct attcttgatg
    21961 taataatcat aactataaat ccaagtacag catatattac attctttttt agaaaatagt
    22021 acgcatcatt gtgtttaaat gaagattgta taaaacttgc actaaacacc ataactattc
    22081 ctacaaatac taaaagcata gtagtgtaaa acactactcc atcaaattca gtcttcatcc
    22141 ttatgtcaat ttgttttttc aaattttctt taggcatcca actaatctac ctccattctt
    22201 ccatttaagt aagtttagag gtaagagatt attttaaatt gtttacatta tctttgaagt
    22261 cttttcctct tacttcaaaa ctttcataca tatcccaact tgcacaagct ggtgaaagaa
    22321 gcactatatc tccacttttt gaaatttcat aagaagtctt aactgcttct tccatatctt
    22381 ttactatatg tatgtcatta aatcctttat ttttagcaca attttctata tttgatgcag
    22441 tttctcctaa taagaccaat gatttaacat atgactttgc agtttcaaga agttcatcaa
    22501 aattacttcc cttatccatc ccccctgcta taagtattat aggtctatca taagactgta
    22561 tagcttttat agtggaatct ggattagttc cctttgaatc atttacatat attacattgt
    22621 ctaaatttct tacaaactct tgtctgtgtt ccactccttt aaatgtcata agtacatcct
    22681 ttataacttc taaatcaatt ttgcagacat atgctattgc tatagctgcc atacaatttt
    22741 ctaagttatg acctccaggt aaacttacat catctttatt tagaaatttg attttatcat
    22801 ctatatctat tattatgtca ttattttcta aatatactcc tccatttact tttttagttc
    22861 ttgaaaagta aattactttg gcattgcatt tatcagctaa agattttact acctcatcat
    22921 catagtttaa cacacagaaa tcctcaacag tttggttcat aaatatattt gcctttgctt
    22981 ctatgtattt ttccatagta tggtgtctat ttaagtggtc ctctgtaata tttaatattg
    23041 cgcttacttt aggtctaaat tcatctatac tctcaagctg aaaactgctt agttcagtta
    23101 ctaacacaga ctcttcactt gaagtttcta tagtatctat tacaggattt cctatattac
    23161 caactacata tgtatctcgt tttgctcttg aaaaaatttc tcctaccaaa ctagttgtag
    23221 ttgtctttcc atttgtacct gtaatcccta taaaaattgg attattcgat agtttaaaag
    23281 ctaattctac ctcaccaatt atatacttgc ctgaattttt taatttcaat atgaatggta
    23341 ggtctagtgg tacacctgga gacactacaa ccatatctat atcatctaca tcttctgggt
    23401 gatgtcctaa tatgtatttt atgtcattta tagatttcaa ttcatcaagt atatttctca
    23461 atttattttc atcttttata tcatttacga ttatacttgc acccaattta tctaaatgtt
    23521 taattgtaga tataccagtt tttgcaagtc ctactaataa aacttttttt ccttttaagt
    23581 ccatttttca ttccccctag ccctaaaata ctgctattat acttatccaa gctaaaacaa
    23641 ctgttataat ccaaaaaata aatacaactc ttgtttctgg ccaaccacac tgttcaaagt
    23701 ggtggtgtat tggagccatc ttaaagaatc tctttcttgt tgccttaaaa tatccaactt
    23761 gaatcaaaac tgatagagcc tccgcaaaat aaattcctcc aattataggt attataagca
    23821 ctgagttagt tagaactgaa aatgctacaa ctgcacctcc taatgccatt gaacctgtgt
    23881 cgcccataaa cactctagcc ggatatgaat taaatcctaa gaaacctaaa catgctccaa
    23941 cagttgctgc tgctaaaact gcgacttctg tatttccagc aatagaactt gcaaatagca
    24001 taaagaatac agaaactatt aatgttactc ctgatgctaa tccatctaat ccatcagtca
    24061 agtttacagc atttactatt gctacgatta taaacatcat tattggtata tataatattc
    24121 caacatttat cacaaaatct gtaaatggta tcataagttg tgaagcactt gatgatgatg
    24181 tatactgata aaaagcaaca taaaaagata atgcaacttg aaggattatt ttttgatatg
    24241 cttttagtcc aagtgatctt tttagcttta tttttataaa atcatctaaa aagccaataa
    24301 aaccaaaacc tgcaatacat ataagaccta ctgccatatc atgactaact tttactcttg
    24361 taagtccagt tattagaatt gcaacaatca ttataattcc acccatagta ggtgttccgt
    24421 ttttagctag atgtgtttgg ggtccatcat ctctaactgt ctgaccaaat ttaaattttc
    24481 taagcattgg tataaatatt ggtcctatta taattactat taaaaacgca atcaatgcgg
    24541 tgtacgtaag ctctgttatt cctaacatca taaaactcct ctcccttgtc aattaactca
    24601 ttagtttaag cttatttatt tagatattcg actatttttt ctaatctcat gcctcttgag
    24661 ccttttacta aaatagtgtc tcctgttttt actaattcat ttaacttgtt aaacacatct
    24721 tctctattct caaagtgata tatattggct gaattgaatc ctaattgttt tgcttcttct
    24781 ccaataaaca ctgaattttc acctatagtt attataatat cagtattatt catagatgat
    24841 tttccaacta atctatgacc atactctgaa atttcaccca tttctagtat atctccaagt
    24901 atcgctaccc ttctaccttt atatcttcca agtatcttta aagcagcatc catagaatct
    24961 ggactagcat tatatgaatc attaattatt gttaattttt cattttttat tatgtctagt
    25021 ctcatttttg tccctttaaa attttttaaa ccttttttta tataatctaa agaaatattt
    25081 agacacaaac caactaatat agcagacatt gcattatata tattatgttc tcctattgtt
    25141 ggtataaata tttcctcttt tttcccattt attacacata taaatgttaa actatcctct
    25201 tccatagtat aactttcaca ataaatatca ttgtttttat taaagccaaa tgtttttaac
    25261 ttatatacat gttctttttc ttttaatgta gataaaaact tatcatctcc atttacaatt
    25321 aaagtatttg tttcatcaaa gtttgaagta atctccatct ttgctttcaa aataccctct
    25381 tgtgaaccca gtttttctac atgtgataat cctatattcg atatgactgc aattttagga
    25441 tttactatat ccactaaata ttttatttca ttgaatccag acatacccat ctctattaca
    25501 gcacactcat gttctttatt tagattaaat aaagtcaatg gaactccaaa atgattattt
    25561 aagttttttt cattttttag tatatttaac tttgcagata tggcagagta aatcatatct
    25621 ctagtagtag tttttcccac actacctgta acacctataa atggaatatc aaatttttct
    25681 ttatagtatt ttgaaatatc tcctagagcc aaagatgtat cttttacctc tattaaattt
    25741 atatccgaac tttctaattt tattccatta ctttcatttt ttataaaagt tttacatcca
    25801 ctatcatatg caggcttcat aaatgtatgc ccatctaaat tttctccaac tatggctaca
    25861 aatgcatttt gggcacttgc ctccctgcta tctataacta tatcacttac acagtcatta
    25921 taatctccat atattaactt cccattagtt gcaagtacta actcttttat tgttaagcac
    25981 tccattatta aactcctcct ttaaaacctt agctatattc tcttagcaac aaaaggcaat
    26041 gtataccgtt atatatacat tgcctacgat aactcttaac tttataatta tatactatat
    26101 ttctgttttt ttgaagatgt ttatgggtat atgttaatta tctgttactt tttagttttc
    26161 aaaatatagg gttatacttg agtcttcatt aacttttact ccaggttttg ggaatatatc
    26221 ctttaccttt gtatcttctg gaagttctat atctgcatct aagtctggtt ttaacttcgc
    26281 atcctccaat gcctttacag catctcctat ttttagattt cttacatctg gcactttaac
    26341 ttgcttcttt tcatactcag ctttttcttc ttctttatat actggtttaa ctcccaaata
    26401 ttttaaagag tcattcatta tttctttcac tattggtcct gctgtagtac taccaaatgc
    26461 acttactcct gttggttcgt caacaatagc aagaactact atttgcgggt catcacaagg
    26521 tgctattcca acaaatgaac atatatactt tcctggagca tacttaccat ctataacttt
    26581 ttgagctgta cctgttttac caccaagtct ataccctggt atatatgcta tctttcctcc
    26641 accttcagtt acaactgatt ctgcaatttc taacattttt ttagatgttt ctttagatat
    26701 aacacttctt actttttttg gttttactgt ttcagttata tttcctttat tgtcagtata
    26761 agattttacc actcttggtt gcattaaatc tccaccatta gctattgatg atatagctgt
    26821 tataagttgt attggagtta ctgatataga ttgaccaaat gatattgtag ccaattcaac
    26881 tggtcctaca tttttttcgt tatacagtat tcccttagct tctcctggta aatctatacc
    26941 tgtcttatcc ataaggccaa atgattctat ataatcatac atttttccaa cacctaatct
    27001 gcttcctaat tcaacaaata ctgggttgca agagttttgt actgcttgtt taaattcttg
    27061 tgttccatga ggtctgtaat gtctccaaca tttaatcttt cttcctccaa cagttacact
    27121 acctgtacat gtgaattttt ctccatcttt aattactccc tcttcaagag cactggaaga
    27181 tgtaataagc ttaaatgtag aacctggttc atatgtatca cttactgctg gatttctcca
    27241 catttgataa tagcctttta ttttatcttt atcattatat ttttcaagtt cttcttgata
    27301 atatggatat attggcgttc ttgagtcatt agggtcataa tctggttttg atgctaaagc
    27361 aagtatatca cctgttttag ggttcatagc tattgcagta accttttttg cattatttaa
    27421 ctcataagct ttctgtactg ctttttcagt atagtgttga attacttcat ctatagacaa
    27481 cacaagacca ttaccttgta caggctcata atatttttcc attccttgtg gtatttctct
    27541 tcctgaggca tcagtactta ctataagttt tccaggcttt ccttttaact ttttatcata
    27601 ttgcatttct acaccagata tacctgttgc atctgaagaa gtatgtccta acacatatgg
    27661 cgcaaaattc ccatatgggt aatatctttg attatcttct gctacccata ttccacttaa
    27721 tttagcatct cttatttgac ttgccttatc atcatctatc catcttttta cttttacaag
    27781 agccatattt tttttgctta ttaaagcgta aatatcttta taatcttcat ccaaaatctc
    27841 agctaccttt tcagctgctt cttttttatc ttctacttcc acaggtttac accaaacagt
    27901 atacttagtt acacttactg ctaattcttt catatttcta tcatatatag ttcctctttt
    27961 gggttctatt ggtatatctc ttgtttgttg ttctagtgct tttgtactca accaatttcc
    28021 cttcataagt tgtaggtacc cagtcctaat aacaaggcaa aaaaacaatg cacatgctag
    28081 aataagaacg agtaccagcc ttttcttact tatcctcttt acttttctca aatctattcc
    28141 ccctagttat ccacatctat atactttatt tgtccatctt ttggatagtc catattgagc
    28201 aattccttag cctttagctc aatttctttt attgatgttt tttcagtttc ttttgccttt
    28261 agttcctcta atactatctc tttttttctt aaatcttttt gtaaataata gatatcgtac
    28321 ttcatttccg agatttttga ataaccacat agattcaata ttattactga aaaaattgca
    28381 atcagtaaaa tgcatttagc tcctattttt ttcttatttc tctttttata attattcttt
    28441 tttttatttt tatattcatt tagattatgt attttataat tttttttcaa attctcaccc
    28501 cgatagtata tagtttaaat tttcctctat actatatttg ttattaatca tatttcttcc
    28561 tctaaaattt catcgctaaa ttaatacact attacacaat ctaattgtat ttttttctat
    28621 acacaacaaa aacctataaa ttcacttata cttttatatg aatctatagg ttagatatta
    28681 atttctattt atttttatca cttctagtat ttttagataa tttcttccat aaatataata
    28741 tgtaactaac tatctactct ctgattccta agttctccat tatattactt aataccatct
    28801 atatctatat ttttatatct aaattttcat tatatatctc aataacaaag aacatttctt
    28861 atagtgttaa ttgtttttta attgctttac cagttcaaca aaatatataa tactttttaa
    28921 ttaattatag taatcttatt ttatatataa gattattctt tagccttata actacttata
    28981 ttttttctgc tactcttagc ttagcacttc ttgaacgtgg atttacttct atttcttcct
    29041 cactaggtaa tataggtttt ctagttatta ttttcacctc agattcttta tcacattggc
    29101 atataggtaa atgttgtgga catatacaat ctgatgctaa atgcttaaat gcatttttca
    29161 ctattctatc ttctaatgaa tggaatgtta ttatacatat tcttccacct ttattcatta
    29221 tagaagaagc atcatttatc atctttgtaa ttactccaag ttcattattt acttctattc
    29281 gtattgcctg aaaagttctt ttagctgggt gaggtccatc tattctagct ttcttaggga
    29341 tagctttttt aattatatct actaattttc ctgtagtttc tattggtttg tttgctcttt
    29401 cttcaactat aaatttagct atacgcttag cccagttatc ttctccataa tcttttatta
    29461 tcttagttaa ttcatcttct gtataagtat ttacaacatc atatgcagaa aactcacacc
    29521 taacatccat tctcatatca agtggtgcat cttgcatata agaaaatcct ctatcagctt
    29581 catcaagttg atgagatgaa actcctaaat cagctaatac accatcaatt ttgtaaactc
    29641 ctattttttc taattcctct tttatatttt caaaattgct gtgaacaaac ttcactctac
    29701 tttcatattc actcaatctc tcttttgctg tgcttatagc atttttatct tggtcaaacc
    29761 ctataaataa tcccttatct gacagttttt ttactatttc tttagaatgt cctgctcctc
    29821 ccatagtaca gtcaacatac acgccatctg gctttatatt taaattctct atacactcat
    29881 ttaaaagaac tgatacatga tgaaattcca tagttacctc ccttaaattc actttttaaa
    29941 ctattcttca ctaatattat gtttattttt ctttgataaa taaacatgtg caattatgcc
    30001 accaataact ccaattagac ttataacttg tgccattcta agtggtccta acattaaact
    30061 atctgttctt aatccctcta taaaaaatct tcctattgaa tataaagtta tataagtaac
    30121 tataacttga ccttcatatt ttttattttt tctaaatagt aataaaacta taaatattcc
    30181 aaaatcccat atggactcat ataaaaaagt tggatgtacc tttactccat caaccattat
    30241 tccccaaggt aaattggtag cacctccatg tgcttctcca ttaataaaat ttccccatct
    30301 tccaatagct tgtgctaaag gcattccaag tataactgta tcagccattt ttaagaagtt
    30361 aatattttta atcttagtgt atatataacc agctaatatt ccaccaatta accctccatg
    30421 tatagcaagt ccaccacctc taaaattaaa aatttgagac atattttgag catagtaact
    30481 ccaattaaat acaacataat agattcttgc acagattagt ccaactggaa ttgctattat
    30541 agctatattt aatacatcat catctttaat tcccactctt tttgcttctt ttattgctat
    30601 taaagttcct aaaatcattc cacatgccat aagtattcca taccacatga tgtctatgcc
    30661 aaacagtgta aatgctactc tatccataac gtcacctctt tgtattaatt gaaaatattt
    30721 ccaaagcaat atgctataat tacaattaat tgtaattata ctaaattatg ataacacatt
    30781 aatgttaatt ctacaaaaaa tattaaaatt tattctacaa atccctacac tatattatac
    30841 taatataatt actataaaat tttttaaact gaatctctca tgactaaagt tacaagtgta
    30901 catagatatt ttttaatcaa aaagattgat gcatatatac atcaacctct tctattcctt
    30961 atctatagct cttaaatttt tacctcaatt tatataaata tattttataa atttgtttta
    31021 ccaaaataaa catttaaaaa cataaataat acttaattag ttattcttat tatgattaag
    31081 tttctattta ggttctacaa tagaaataac acaatactct tctttaaaat gctcttttaa
    31141 tctttcttca acttcctcaa aagtaatatc tttaataaca tctaaataat ccaatagatt
    31201 tatatcctta aaaacataag atataaaact attacctata aagtttatag aatcaaagca
    31261 cttaataaat gaccctattt ttttcttttt agttctctca aactcttctt ttgataatcc
    31321 ttcttttttt gatttttcta tataatcaag aattatttct tttacttttt taggctcttt
    31381 agaatctcca gctattatgc taaaagcata atccacttga gacgaaaatc cagctccaaa
    31441 attttcattt attaaacctt gcatatacaa atcttcatag agcttacttc cttttttaaa
    31501 taacatccct acaagtatat cagttacaat ctctttacgt aaaagttctt tcccttttaa
    31561 accaacatta ctatctttaa aacctatgtt gaacataggc atagatatag ggaatttttc
    31621 tataacctct ttttctttta cactttctgg ttcttctgga taaaatcttt ctatactctt
    31681 agagagtttg tctactttgt aattgtttga ctttttagtt acatctataa ctttttctac
    31741 atctaagtca ccaacaacaa ataaagccat atttcctgga ttatagaaag tattataaca
    31801 tttatatagc tcttccttag ttatcttata tatactatca acagtacctg ctatatcaat
    31861 tctagcagga taatttacat acattgcctt caagcaatta aagtatacat tccaatcagg
    31921 gtcatcattg tacattttta tctcttgtgc tattatacct ttttcctttt caacattttc
    31981 atctgtaaaa taaggtgttt gaacatagtc aattagatgt tctaaactct cataaaaatt
    32041 ttcagtagca gaaaataagt aggctgtcat tgtaaaatta gtaaatgcat ttgcatttac
    32101 tcctaatttt gaaaatttat caaatgcatc tcctccatca ggttgctcaa acatcttatg
    32161 ctctagaaaa tgtgcaatac cttcattcac tctaattttc ttatcttctc caattggtac
    32221 aaattctaag tcatttgaac cataattagt agccaaaata gcatactttt tcataaaacc
    32281 ccttttaggc ataaaatata catctaatcc attttgcaat ttctcataat aaacttcttc
    32341 tttcaatata tcattaacta ttttttccat agacatctcc tccattaatt tctcaagaag
    32401 tatacagtat caagctctat attttttata gcttcaacta tatctttttt tgtaaccttt
    32461 tctatactag atattatgtc ttgtacttca gaatttgtct ttgccataga ctgagagaaa
    32521 acaaaatcag acataccacc tatattatca gttatagatt tcatagaatt aactaaagca
    32581 agtttactgt tttctaattc ttcatcagat atatcacctg cttttacact ttctacttgt
    32641 ttttttatca attctacagc tttatcataa tctttagttt ctataccaga tgatataaac
    32701 atgatacttt tatacttctc tattgaagag aatatatagt aacataaact ttctttttct
    32761 cttacattaa caaataattt agagtgtgga ccaccaccta agacattgct cccaactact
    32821 aaagcataat atttatctat gtctgcataa tccacatttg ctctataacc cataactaac
    32881 ttaccttgag ttatttccat ttgttcatct ataactttta cctcatccac attttttata
    32941 aaatctgctc ttggtatatt tataatttct tctctttcaa attttagatt tttacttatt
    33001 atatctacaa ctttatcttc atcaaaatta ccttctacaa ctatatctat aggtgatgtt
    33061 ttaagtatgt ttttataatg ttcatataac tctcttgaag ttatactatc tatttcatct
    33121 tcataaccaa attcacttac actatatctc tctcctttac acatagcctc aatacattta
    33181 tcttgcgcat attccttttt atcatttatt attgattgta tcttttctct taaattctct
    33241 ttttcaatat ttaaatattc ttctttaaac ccaccatcta caacaagcgt attatttatt
    33301 acttcattaa aaaattctac tacttcttca aatatactct catctaaata cttttcactt
    33361 atgttaatta ctttaaaact caatacttga cgttctccac gttttacagc atcagctccc
    33421 atagatgaac catataaatc atctaattta ttggatattg ctctagcact aggatattta
    33481 gcgcttccac ttgctattat tgatggtatt agagcatttt ttgtagtttc tttttcatcc
    33541 aatagtctct gaacatataa acttataaga ttcgatttaa acttttctgt ttttatcaaa
    33601 gtaagattaa tattattacc taaatttatt atttttttag tattaaccat atgtacctcc
    33661 tatttttatt taaacatcaa ttttaaaatt gaaatttatt tattttttaa ttatatatac
    33721 tcacatataa aaaccttcta tttaattata tcctttttat tattttctaa aagcaatatt
    33781 tttatttaaa ttaaatgcaa atattattta aatcaatatg ctttaaagtc tagtttacaa
    33841 gcttgatata tatgatgtat aatcaatatg tatgtctata attaaaacaa ttttgcaatc
    33901 ctatagattg attgtttaaa gttatataaa gaatataaaa aagaataaga gagaggacga
    33961 ttaatatgaa attaggtata gttggtttgc caaatgtagg taaaagcaca ctatttaacg
    34021 caataactca agcaggtgca gaatctgcaa attacccttt ttgtacaata gacccaaatg
    34081 tgggagttgt atctgttcca gatgaaagat taaacaaact acaagaatta tataattctg
    34141 aaaaaatagt tcctactgct atagaatttt gtgatatagc aggtttagtt agaggtgctt
    34201 ctaaaggaga aggtttagga aataaattct tatcacatat aagagaagtt gatgcaatag
    34261 ttcatgttgt tagatgtttt gaggatgaaa atgttgtaca tgtagatggc tctgtagacc
    34321 cacttcgcga tatagaaact ataaacttag aattaatttt ctcagatata gagattcttg
    34381 aaagaagaat taataaaact caaaaagctg ctaaggctga taaaacttta ggttctgaac
    34441 ttgatttatt aaaatcaata atgtctactt tagaagaaag taagtgtgta agaactatgg
    34501 aatttacaga agatgaacaa acattcgtaa attcattgga tttacttaca tctaagcctg
    34561 taatatatgc ttcaaatgta tcagaagatg atttagctga taatggagaa aataataaat
    34621 atgttcaaca agttaaagca tttgcagaaa cagaagatgc cgaagttgtt gtagtatgtg
    34681 cacaaataga agcagaaatt tctgaacttg attctgctga agaaaagaaa gagtttttag
    34741 aaacattagg tcttgaacaa tcagggcttg ataagcttat aaaatcttct tatgctttac
    34801 ttggtttaat ttcattctta actgctggtc caaaagaagt tagagcatgg actataaaag
    34861 ttggaagtaa agctcctcag gcaggaggaa aaatacattc tgatatagaa agaggattta
    34921 tcagagcaga aacaatagcc tttaatgatt tggttgagca tggtactatg gctgctgcta
    34981 aagaaaaagg gttggttaaa ttagaaggta aagaatacat agttaaagat ggagatgtaa
    35041 tattatttag atttaatgtt taaaatgatt aatcatatac actttgacta ttatatataa
    35101 taactaaaaa actaagatta taataataca atattatctt ataataatta tatagaattt
    35161 aaaaataaaa gaagagtatt tcaaatgatt tgtataaatt attatgaaaa actctttttt
    35221 tgatataata aaattactta catatataac atttaaattt ataaattatc tttttatttt
    35281 ttaaaatatg tataacacac ctatagaaat tactattatt actatctaaa gtattttgct
    35341 ataatcccat atactcttac ctcaaaaaca ttcaaatgga aaaaatacta catattaata
    35401 aactttgccc aataattcct aaaataatat cctaacaatg atttatgttt ttataatagt
    35461 atatttttac ataaaaaagt gaacattgat taacaaagtt cactttttta taaattttta
    35521 tgttataatt cacaatttta tttaatttgt ttcttttcta tttaaactat atctatctaa
    35581 tattaatttt tcatattctt ctattggcat tggtctagca aataaaaatc cttgggccat
    35641 atcacaacct ataaacctta aaaactctgc ttgctctaca gtttcaacac cttctgaaac
    35701 aacttttata tcaagttgct ttgccatttg aacaatactt gaaactacaa tttgactctt
    35761 ttttgcattt cccactgtta taaagaattg cctatctaat ttcaatacat caattggcat
    35821 atctttaagc atatttaaag aagaataacc agaaccaaaa tcatccatag aaattttaaa
    35881 tccaacagat ttcaaagttt gcattatttt aaataatatt tttgtattgt caaatattgc
    35941 actttctgtc aattcaatct caattaaatt tggttcaatt tcatattctt ttgttattct
    36001 ttcaagctca gaaataaaac ctggattctc aagatgtact cttgataagt ttattgatat
    36061 aggaaatgtg gggaaatttt cattttccca acgtttaaaa tttttacaaa cttgggcaaa
    36121 tacaaacata tccaattctg ttataaaacc atttttctca aaaataggaa taaatacacc
    36181 tggacttatt agaccttttt tgggatgttg ccaacgtaca agcgcttcag cacctacaat
    36241 tttagtagtt gataaatcaa ccttaggctg taaataaact ttaaattgtt cattttttaa
    36301 agcatcatgc atttcttgtt caatatcacg ttcttctatg agagtttctt ttaaattttc
    36361 agcataaatc tcataaactt ttaaatattt tctttttact gtagtttttg caatcatagc
    36421 tttgtcaaga caggtagaaa tatttgtttc tccctcttca acaaaatata cacctatgga
    36481 tggaattaaa ttgagttcta ctcccaaact tgtttttatc attgacaatt ttttacgtat
    36541 aagttcacat atttctataa ttgactctct attatttttc ttttcaaata ttatagcaaa
    36601 attgtcattt gaaattcttg aaaatacaga ttgctcttta aatatattct taattatctc
    36661 tgctatttcc tttaatactc tatctccttc ttcataccca aacctatcat ttactagctt
    36721 aaacttatct atatcaaaat aaattaaaac tagattcttt ttctcatgag aggataaaaa
    36781 ttttcctcct tcaagattaa atttatttga attgccaata cctgtaacgt tatctttaaa
    36841 agcaaattct tccaattttt ccttactctt tttatgtata aatagtatat aaattgttaa
    36901 tgccgtaaag attataatca acactacaca tgctgataat gtaagtttta taatagcgta
    36961 tgatttctca gatatagcag ttttaggaac ttcagaaaaa agataccaat cattgattcc
    37021 tattggggca taacttaaat aatatccttt tccaaacata ctataactta atgaacctga
    37081 tttaaagttt tttatatttg acttcatatt attgatacta gattttgata ttgaatcact
    37141 tctgccaatg taagaaaata aattttcttt agtagagttg gcgtttttgc tgcttggatt
    37201 taaaacaata ttaccattac ttctagctat ataagaatat ccttttccat taaatgtagg
    37261 tacagaaagt attttttcat acaacttcgt actatgtgtg gcaaatataa ctcctttaac
    37321 ctcattatct ttataaattg gcacagaata tacattaata ttactattgg tatttttatc
    37381 atcttctatt acatctttta atttttctgt tataaccact tctccttgca ttgattttaa
    37441 gaaataatct cttttagaga agtcttctat ataaccatct gtagtgtatg ctgtaccatt
    37501 tggtaaaata actcccattc ttttaaatga actattaata gcttgtcttt ttagtataga
    37561 aaggatatta tctacttcaa aagactcttc tccctcaata aatattgaaa ttgactctag
    37621 taactttata tctccattaa tttgtttttt taacacattg acgctttgat ttgctatttc
    37681 tgaaagatat tgattcgttt cttcacctaa tgtcttattc aaccttgaac cataaatcaa
    37741 acaggaaatt gtaattacta agatacttat gacagcaatt atggacaaac catacctatt
    37801 attttttgca tcacttaaca ttatgcttcc tgcctttctc gaatctatgt atgtataatc
    37861 attatcttat gacaattatt gtaaacattt tataataata cttatttttc catatttact
    37921 gtaattatac aaaattataa actttattac aacaataaat taattaatat agatattagg
    37981 gtttatatta ttgcattatc tattttacaa tcatttacat ataaaaaaat gtaatatcaa
    38041 agtgaaactt gatattacat ttttgttctt gtattttgtt taatttttat gctacatttt
    38101 acttaaacac tcatttgcaa cttctttatc atcaaaatga tgcttaactt tacctataat
    38161 ttgataagtt tcatgtccct tacctgttat aattacaaca tcatcctttt ttgccatact
    38221 tatagcttct gctatagctt gttctctatc aacaacaaca tggtaatttt cttttttctt
    38281 gtcaattcct tcaagtatat cttttattat tgcctctggt tcttcagttc ttggattatc
    38341 agaagttata atacataaat cagaatattt ttgtccaatt gctcccatca aaggtctctt
    38401 ttcagtatct ctatctccac cacatccaaa cacagaaatt atttttcctt ctgcaaattg
    38461 ttgagttgtc tttagtacat tttcaagtgc atcaggagta tgagcatagt ctacaattac
    38521 acttatacct ttatcatttg gaactgtttc aaaccttcct gatactccat tagaaagtct
    38581 aagaccttct ttatatattg gtttaggtat gccaagtaca tagcaagcag ctataactgc
    38641 taaagtatta tatactgtaa acattcctgg aactggtata aatattactt cttcatatga
    38701 aggtgttatt aatctataag atactccatc cgaatctgat tttatatctc tagccataaa
    38761 atcagcctta gtatcaacac cataagtgta acaaggtaca tttatacctt ttatattttc
    38821 atatatcttt tttccacctt catcatctat gtttattata tttgccatag tagtcttaaa
    38881 aaataatttt tcttttgctt ttctataatc ctctaaatct ttatgaaaat ctaaatggtc
    38941 tggtgttaaa tttgtaaata tgcctagttt aaagtctgtt tcatcaactc tatttaatgc
    39001 caatgaatgt gatgaaactt ccatcgcaca gtagtcacat ccattatcca acatattatt
    39061 aaaatgatat tgtaaatcta tactttctgg agttgttgaa ttggaaacaa tgtctttttc
    39121 tccgtcaaaa atttttatag ttcctataag tccaacttta ttcttattta aagttaatat
    39181 ctcatttagt atagtagtta tactagtttt tccattagta cctgtaactc ctataacgtt
    39241 aaatttctga gatggatgat tataaaaatt atcagctact tttgccatat cttttcttgt
    39301 atcttttacc tttataaatg tacatccttt tatttctaca tcttcttcaa caagaaatgc
    39361 acttgctccc ttttctatgg catcttttat atacttatga ccatctgaca caaatccttt
    39421 tatacatata aataatgtac cttttgtaac ctttcttgaa ttatattgta cattattaat
    39481 atctatattt aattcaccct tgacattgat tatatccaac ccctgtataa tatcatttaa
    39541 tttcataaat gctttcctcc ctttaaactc tttctgctaa aattacacta catataatat
    39601 tatattaaga attttttact atgtgaatgg ttaaaaatgc ttgaaatatt gcaaaaaatg
    39661 ctctataatt ttctataata taactgagga aaggtcttct ataaaaacta tggataataa
    39721 aatcgggtac ttaaacgata actttaaaat atttaatatt agagataaaa aagatataca
    39781 atttgaatac cataatcatg attttaataa gataattata tttataaatg gaaatgtaac
    39841 ttattttgtt gatggtattc cctataaact aaagccttgg gatatactct ttatcagcaa
    39901 taatgaaatc cataaaccag ttatagattc aaatgtcttt tatgagagaa ttgcaatatg
    39961 gattagccca gaatttatga aaaaaaatag tgatgctact agtgacttag aaaaatgttt
    40021 taaaatagct acggagaata aatgcaatag attgcgttta aatatacatg atattgaatc
    40081 tattaagagt tttattgagc aaataaatat ttccgaatcc aatactgaat ttggaaatga
    40141 aatattgaga a
//
LOCUS       DAEPLF010000015        39463 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEPLF010000015 DAEPLF010000000
VERSION     DAEPLF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.015"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            70..486
                     /locus_tag="KR314_000688"
     CDS             70..486
                     /locus_tag="KR314_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327091.1"
                     /translation="MSKSETGVKSKLILELLVITYLFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSKFILSKVNQIAITIMMYSIVIIAIFFATPYISNI
                     NISNLDTGIILIAVLFIQSLSKLILYIYFDKRGICN"
     gene            487..699
                     /locus_tag="KR314_000689"
     CDS             487..699
                     /locus_tag="KR314_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0327092.1"
                     /translation="MAISMKTKIKEYREKLLMTQNELAKSVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFGTTVENLFEFIEED"
     gene            1334..2377
                     /locus_tag="KR314_000690"
     CDS             1334..2377
                     /locus_tag="KR314_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBH0327093.1"
                     /translation="MKRKIAVVLLTVAMLLSLVACSNDNTSKSDKLEDTLVVYTTHGE
                     DMLGAISEEFTKETGVNVEFINLKGELADRVRSEKDNPQADIMFGGDTSTYMQLKEEG
                     LYEKTEPSWNNELDKSYKDSDGYWYGTIKTPVMMFYNTKLLSADKAPKDWSDLTKEEY
                     KDLIVTRDSLSSSMRSTISNLINYYSNTKSEEEAWKYLEDLNKNIKNYYNSGSVMYSA
                     IGKGEATISVAVLSDIIENKEKNNMPLEVVDAESGSVVITDCVAALKNAPHPNAAAKF
                     VDFVGSAKVQAMVANDFNRIPTLDSALKDSPKWMSKEYKAMDVDWSVIGKNQSNWIEK
                     WETDIIDNNKTIK"
     gene            2638..3512
                     /locus_tag="KR314_000691"
                     /pseudo
     CDS             2638..3512
                     /locus_tag="KR314_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3531..4523
                     /locus_tag="KR314_000692"
     CDS             3531..4523
                     /locus_tag="KR314_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327094.1"
                     /translation="MKNIELKEINHYYGKEKVLHDINISIKEGEFFTLLGPSGCGKTT
                     ILRIIGGFIKPSSGSIYVGDKNITNLEPENRNMGTVFQNYALFPNMTVEENVSYGLKI
                     KKLSKKIIKEKCDNYLELSGMKDFRKKKIDELSGGQQQRVAVARSLATEPTMLLLDEP
                     MSNLDIALRIKMREEIREIQQKTGITTLFITHDQQEALAISDRIAVMDKGKVLQIGVP
                     IEVYKSPLNDFVANFVGTSNCIGKEDYVNFNIEKETKPYIYKRPEEMILLQNTNQSGF
                     VKVKVESKNFLGAILEYTVSNDGKKYQVTELNRLNNSNKLNVGDMAYLGVLQGD"
     gene            4525..6219
                     /locus_tag="KR314_000693"
     CDS             4525..6219
                     /locus_tag="KR314_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBH0327095.1"
                     /translation="MIKKINRSKFTSKNVIFIVLYSILFWILIGYIIIPIGNTFVQAF
                     QGKGGYSFEVFKEYFSNTNNLRVVSNTIILGLGSVFVCGIMGISLALYMTFVCDKHKK
                     IIHILLLSPMMIPGVIMVIACIQIYGESGIVTKSIEILLPFIKKPYDFSGLKGILFVV
                     AYTQYVYFYLNVYVALKYVDYSTVEAAKGMGASKLRVFFDVIWPVITPAVITSTIITF
                     ASGISSFSAPNLIGGGFKVLSTQIVRSKANNHMDIASVQVITLLLMGILFMILLQYYG
                     KKYGVVPSLKSQSYKVHNRKKDLFTFVCKVIIEIQIIMIMLPIVTIVYLSFVSTHSIM
                     TTIFPRDFTLENYQMIFQKSRVLQPILNSFKMSLMAVAVGLIITVPVSYLVIKKDTAY
                     NRLAKFIIMLPWSMPASAIAVNLINTFNQKSIFAFNESLIGGFYIVPIAYTIYALPLL
                     FSSNEVAMKSINLGLEESSRSLGAGRIKTFYNVIVPNMMPGIISGAILVFIRTIGEYT
                     MSALLYGVYNRPISISIVTNMQEFKIGVSMAYGVLVIGICYIALGIIFKLDRKRFT"
     gene            6770..7648
                     /locus_tag="KR314_000694"
     CDS             6770..7648
                     /locus_tag="KR314_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBH0327096.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQGANEKNAPV
                     ILAIHPNELSFLGDSFVVSCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            7684..9579
                     /locus_tag="KR314_000695"
     CDS             7684..9579
                     /locus_tag="KR314_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0327097.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSETKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFPPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVAKPTIRSENS
                     EVELLGA"
     gene            9847..11754
                     /locus_tag="KR314_000696"
     CDS             9847..11754
                     /locus_tag="KR314_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0327098.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDILSESEKVSSIMIENMSQFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            11744..12193
                     /locus_tag="KR314_000697"
     CDS             11744..12193
                     /locus_tag="KR314_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0327099.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNISERLLNVHTKKEILDIFYEV"
     gene            12293..13180
                     /locus_tag="KR314_000698"
     CDS             12293..13180
                     /locus_tag="KR314_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0327100.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGGASK"
     gene            13585..15033
                     /locus_tag="KR314_000699"
     CDS             13585..15033
                     /locus_tag="KR314_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBH0327101.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIIHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            15059..17449
                     /locus_tag="KR314_000700"
     CDS             15059..17449
                     /locus_tag="KR314_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBH0327102.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGIVKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIELKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKDWI
                     NAAQNIYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLNYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKKDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGKNISILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            17451..18122
                     /gene="pgmB"
                     /locus_tag="KR314_000701"
     CDS             17451..18122
                     /gene="pgmB"
                     /locus_tag="KR314_000701"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBH0327103.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLERGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            18161..19561
                     /locus_tag="KR314_000702"
     CDS             18161..19561
                     /locus_tag="KR314_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0327104.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNENKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFINKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKESTDNVVEEV"
     gene            19878..20720
                     /locus_tag="KR314_000703"
     CDS             19878..20720
                     /locus_tag="KR314_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0327105.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            20865..22793
                     /locus_tag="KR314_000704"
     CDS             20865..22793
                     /locus_tag="KR314_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0327106.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKFVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMTIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVASPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            22854..24281
                     /gene="ascB"
                     /locus_tag="KR314_000705"
     CDS             22854..24281
                     /gene="ascB"
                     /locus_tag="KR314_000705"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBH0327107.1"
                     /translation="MSFKDDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYRGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLVGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     INYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            24692..25468
                     /locus_tag="KR314_000706"
     CDS             24692..25468
                     /locus_tag="KR314_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0327108.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IVKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            25484..25849
                     /locus_tag="KR314_000707"
     CDS             25484..25849
                     /locus_tag="KR314_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0327109.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(26015..26764)
                     /locus_tag="KR314_000708"
     CDS             complement(26015..26764)
                     /locus_tag="KR314_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBH0327110.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     GKPFIFVCPTYAWRLPIVVTDFIKETKFLGDNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNS
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(27209..27766)
                     /locus_tag="KR314_000709"
     CDS             complement(27209..27766)
                     /locus_tag="KR314_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH0327111.1"
                     /translation="MRTLKYAILGLLNRNPMTGYDIVKEFNFQLAEFWNAKHSQIYPE
                     LKKLVNEKLIVYDIKISGDVLEKKLYTITEKGKDEFLKWLEKDEPMERTPKDIFRLRM
                     YFSNNLDIDTRLILLEHQLLQHQERITFLYSQMERYSEVPPLDSNDFGDYIVLDGAII
                     REQGAIKWLTNCINHHKNLDNTTLK"
     gene            28018..29316
                     /locus_tag="KR314_000710"
     CDS             28018..29316
                     /locus_tag="KR314_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HBH0327112.1"
                     /translation="MNLTQKRWIILIASCFINLCIGSIYAWSVFASPMAEHLNSLAGT
                     TFTAASLAIVFTVTNSVGPITMITGGMINDKFGPKKVVFVGGLIFGIGMILAGFSKGL
                     PMLILSYGIITGLGVGMVYGCTISNSIKFFPDKSGLVGGITTAAYGFSSVIMPPVANW
                     IINKYGVTSAFKSIGLVFLIIVCVSSFFIKKCPADFTPEGWVPPEKKGKVRVVINKNW
                     KEMLSTPIFYLMIIILTCGAFYGLMCVSLASSLATNMIGMTSSDSAIVVSILALFNTL
                     GRIVAGYISDKIGRINTLSGALVISIFGLLCLLFSREGDVATFYIGIIIIGICFGSFM
                     GIFPGFTVDKFGPKNNSVNYGIMFIGFAFAGYIGPAVMKNVYSVDGNYQRAFIIAIII
                     ALIGFILTFLYKYINKIQNLKIENLIKGDIKNGKKISKSM"
     gene            29288..31237
                     /locus_tag="KR314_000711"
     CDS             29288..31237
                     /locus_tag="KR314_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0327113.1"
                     /translation="MERKYPNLCKPIKIGNVTFKNRMFSAPMGGTDITADCTIGSKST
                     AFYELRAKGGAGAVTISECMVHPETDGSHAYHLDLKIVDSLASFTYTADAIRRHGAIP
                     SVELSHSGQYSGTYLVDKDKKRGLSQWGVSPSVRPDGLEIKELTEEIIAEIVESYGNV
                     AALAKRAGFEMIMIHGGHGWLINQFLSPYFNKRTDKYGGSLENRVRFAKEVLDSVRKA
                     VGPGFPIEFRMSGSELFEGGYDLDYGTEIAKLLESRVDLLHVSAGTYQRGFAVTHPSM
                     FLEHGCNVYLAEEIKKHVNIPVATIGALNDPEMMEEIIASGKADVIYMARALLADHEL
                     PRKVMSNQDEKIVKCLRCFTCMAERATTSTRRCTVNPLIGRELDGTEVVSVLKPKKVL
                     IAGGGPGGLQAAITAVKRGHKVILCEKTNELGGILKGEQALPFKYKMYELGNTFGKIA
                     KDLGVEVRLNTTVTKEYVENENVDALIIAVGSEPLVPPIEGLDGENVVIVNDYYVEKE
                     KVTDEVIVLGGGLAGCEAAIHLAQEEKTVHLVEMRAELAPDANIRHRPILLEEIERQG
                     IYVYTEHKGLSVTTEGVVCMNKSGNKINIPGTSVICALGQKPRREVVDTLLDSAPYVA
                     QIGDCVRASTITNAVYQGHHAALDI"
     gene            31674..31811
                     /locus_tag="KR314_000712"
     CDS             31674..31811
                     /locus_tag="KR314_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0327114.1"
                     /translation="MINENLKSLRKIHQYTQEELAEKLNVSRQSIAKWESGVSQTKGY
                     Y"
     gene            32039..32413
                     /locus_tag="KR314_000713"
     CDS             32039..32413
                     /locus_tag="KR314_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBH0327115.1"
                     /translation="MFNLIEMLFPIMFILIFIIIVFTFVKGIATWFKNNNSPRLTVSA
                     RIVAKRQNTTHNNQPNAGDISGAHGFHTISSTSYYVTFQVDSGDRIELSVSGSEYGVL
                     AEGDKGQLTFQGTRYLTFDREN"
     gene            32429..33076
                     /locus_tag="KR314_000714"
     CDS             32429..33076
                     /locus_tag="KR314_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327116.1"
                     /translation="MNKFLSQIRRIDNEISITRKIINTIFILCFGIVLGTFAKFLDTT
                     ASNTLPFIFEYLDISNFFGRFAIWLLIALYIAIYSHSSIRASLNVLVFFIGMVSSYYL
                     YSYFVAGFFPKNYAMIWLGFTVISPLLAFICWYAKGKSKISFILSVIIIAILFNFTFI
                     YGWIYFDVYSILEVIVFGCALIALKRNTFRETTYMILSAVVIAVILNMLVPFHFG"
     gene            33456..34535
                     /locus_tag="KR314_000715"
     CDS             33456..34535
                     /locus_tag="KR314_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBH0327117.1"
                     /translation="MNDMKFIETLKQKRNVCDYSQSRLAQELQINRQNLNEIENGKTK
                     ASKEMKHILLHYLDYCNCTQPFTLTIDYLRVRFPTTDALEIIKNVLAMKSEYFIHEDY
                     GMYAYEEQYIYGDISVNASKDSSMGVLLELRGMGCRDLEYVLQVRGIDWYCFLRCCID
                     YQGVFKRIDLAVNDMGGLLDIEILRERYYANKVWKRSRTHEAVDSGKLSGTHGDTAKT
                     FYIGSKNSSIYFCLYEKEKEQKSKGIKTDIKNRFEIRLKNGKAGQTVEQLVFSRNPEQ
                     TIASFILTQIDFPDYILWDIFLDNVTTSLPFIMTPVAVNMDKIKRWLERQVMPSLLMI
                     KEIEKKTGANYLEEIDRHTRLTEKQ"
     gene            35357..35570
                     /locus_tag="KR314_000716"
                     /pseudo
     CDS             35357..35570
                     /locus_tag="KR314_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
     gene            35682..37295
                     /locus_tag="KR314_000717"
     CDS             35682..37295
                     /locus_tag="KR314_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0327118.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIVEKIFSLAYQGYGSAKITKVLREEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNTANKTPYSYYACSYYGQFGKGTCSMHYIRYDVLYQAVLKRLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASAFKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITNLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEAITNEDNERIQEIEIYYRFIGKVD"
     gene            37324..37647
                     /locus_tag="KR314_000718"
                     /pseudo
     CDS             37324..37647
                     /locus_tag="KR314_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
     gene            37679..38866
                     /locus_tag="KR314_000719"
     CDS             37679..38866
                     /locus_tag="KR314_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBH0327119.1"
                     /translation="MSKIVFFSILAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIK
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFITHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            39058..39462
                     /locus_tag="KR314_000720"
     CDS             39058..39462
                     /locus_tag="KR314_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBH0327120.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYHALYVNKVMLQI
                     YDVENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT        15255 a         4469 c         7141 g        12598 t
ORIGIN      
        1 ttatttggaa aggttatata tttataacat gaagtgttat aaatatataa cctttaaggt
       61 ggattttata tgtctaaatc agaaactggt gtaaaatcaa aactcatatt ggaattactt
      121 gtaataactt atctttttat agctgtatta gctactaagt atatttttaa aggtccaagt
      181 actgcatctc ttccaggact gagctggtta atagtctttg gatgctattc aatttataat
      241 aaaaaatata atgatttagt tgatgaatca tctaaattta tactatcaaa ggttaatcag
      301 attgcaataa caataatgat gtattctata gttataatag ctattttctt tgcaacacct
      361 tatataagta atataaatat atcaaacttg gatactggta taattcttat tgcagtttta
      421 tttatacaat cgttatctaa attaattttg tatatatact ttgataaaag ggggatatgt
      481 aactaagtgg caatatcgat gaaaacaaaa attaaagaat atagagaaaa actccttatg
      541 acacaaaacg aacttgctaa atcagttggg gtaagacgtg agactattgt acacttagaa
      601 aatggaaaat ataatccttc tttaaagtta gcgatggata ttgctaaagt attcgggacc
      661 acagtagaaa atttgtttga atttattgaa gaagattagt agataggata taaatattta
      721 taagattgtt aataaaatat agttatttaa ttattaattt tgttaaaaat atttataaat
      781 aatataatgt atgagtaaag ttaggttgta attattttaa gataagaaga ctactgtaaa
      841 tgataaaaat tagtgaatat acagccaatt aataaataat agataaaact ttaaagctta
      901 gatgtgagga tgagatatgg tcttacagta gttcacactt ttaattaaga cttaaaatgg
      961 aaaccataca catacataca ctatttactg aaagtggact agataaaaaa ataataagtg
     1021 atttaagaaa gtatctcata tactatataa gtattatttt aggtgaccta tttataggtc
     1081 attttttatg tatataaatg ataataatac taatatttaa aataggtttt aaagaaataa
     1141 aatatccaga ttgaaaaatg gattaatgtt aataaaaaaa ttatatgtat aaaaataggt
     1201 ataattatag aaaaaaacaa taataaaata agctgttata gaaaaataca attatataga
     1261 ttgttgttga atcattgact gcaagaataa taacatttaa aatgaacatg gagaatatgg
     1321 gggaggaatc aatatgaaga gaaaaatagc agttgtttta ttaactgtag caatgttatt
     1381 atcacttgta gcttgtagca atgataacac atcaaaaagt gataaattag aggatacatt
     1441 agtagtatat actacacatg gagaggatat gctaggagca atttctgaag aatttacaaa
     1501 ggaaacagga gttaatgttg aatttataaa cttaaaaggt gaactagcag accgtgttag
     1561 aagtgaaaaa gataatccgc aagcagatat aatgtttggt ggagatacat ctacatacat
     1621 gcaattaaaa gaagaaggat tatatgaaaa gactgaacca agctggaata atgaattaga
     1681 caagtcttac aaagattcag atggatattg gtatggaact ataaaaacac cagtaatgat
     1741 gttttataac acaaaactat tatcagctga taaagcgcca aaagattgga gtgatttaac
     1801 aaaagaagaa tacaaagact taatagttac tagagattca ttatcatcat ctatgcgttc
     1861 tactattagt aacttaatca attactactc aaatactaag agtgaagaag aagcttggaa
     1921 gtatcttgag gatttaaata agaatataaa gaattactac aatagtggaa gtgtcatgta
     1981 ttcagcaata ggtaaaggtg aagctacaat atctgttgca gtattaagtg atataataga
     2041 gaataaagaa aaaaataata tgccattaga agttgtagat gcagagagcg gttctgtagt
     2101 aatcacagac tgtgtagcag ctctaaaaaa tgcaccacat ccaaatgcag cagctaaatt
     2161 tgtagatttt gttggaagtg ctaaggttca agctatggtt gcaaatgatt ttaatcgtat
     2221 tccaacatta gattctgcac ttaaagattc accaaagtgg atgtcaaaag aatataaagc
     2281 tatggatgta gattggtctg taattggaaa aaatcagtct aactggattg aaaagtggga
     2341 aacagatata atagataaca ataaaactat aaaatagcct aattaaataa tttaatatta
     2401 tatacaaaaa tagtgaggat tttcctcgct attttacgta taaggaaact atagtatctc
     2461 ataattttgt ataattcata caacaaaatg atactaatac atacttatta aataaaatag
     2521 tttttgagta actgatgaag taattgacta tataaagtga tttgtagaca tagtctctca
     2581 aataaagtga attttttatt atgcaaaact tttaattata gtaaaagtac aatttatatg
     2641 ggcaaggaga taaaaatgga actaaaaatt agtgattata aattattatg taataaaggt
     2701 agtggcgctt ttaacgaaga tatcgtaggt ttatcacctt atggggcatg ggtacttgat
     2761 ggagctacag gtttaaataa taaaaattta gtctctacag aaagtgatgc caaatggtat
     2821 gtaagttggt ggaatagtta tcttcatgag aatatggaaa gagaagatag cttaaaagta
     2881 attattaaag aaggcattga ggagataaca aaagagtact acaaattaac agatgataaa
     2941 gaagtagaag caatagatat tccttcttgt tcatgttcta tattaaagtt ttataaagat
     3001 aagatagagt atttactttt aggagattgt actttattag ttaacaataa tgaatctgtg
     3061 aaaattttaa aagatgagac tttatgtgaa cttgataaaa aagtatttga ttttatgaaa
     3121 gaagccaaag atgctacaaa ccttacttta aaggagagaa aagccgaggt aatgcgatta
     3181 taatagagaa tagacttaag aaaaatactg tggggggata ttggactttg gaattttcta
     3241 aagaggccgt tgacaattgt gtaagtggat ttattgatat agaatataaa atgaaaatta
     3301 tgatggctag tgatggtttt tcatgtggat atgatagata taagattttt gataaaaatg
     3361 aaatgatgaa aatagcagag attaaaggta tagattatat atatagaaag gttagggaac
     3421 tagaaaagga tgatgaaatg gcaatagagt ttcctagatt taaaatctat gatgattcat
     3481 catgtgtata tttagagatt tataaatatt agataattta tggagacgat atgaagaata
     3541 tagaattaaa agaaattaat cattattatg gaaaagaaaa agtattacat gatattaata
     3601 tatctattaa ggaaggagag ttttttactt tactaggtcc ttctggatgt ggaaaaacga
     3661 ctattttaag aataattggt ggatttataa aaccttcttc tggaagtatt tatgttggtg
     3721 ataaaaacat taccaattta gaaccagaaa atagaaatat gggaactgtt tttcaaaact
     3781 atgcactgtt tccaaatatg acagttgaag aaaatgtatc ttatggattg aaaataaaaa
     3841 agctgtctaa aaaaattata aaggaaaaat gtgataatta tttagagctg tcaggaatga
     3901 aagattttag gaaaaaaaag attgatgaat tatctggtgg acagcaacaa agggtggcag
     3961 tagcaaggtc attagcgact gagcctacta tgcttttatt agatgagcca atgtctaatc
     4021 tagatatagc tttaagaatc aagatgagag aagaaataag agagatacag caaaagacag
     4081 gtattactac tttatttatt acccatgatc aacaagaagc cttagctatt tctgatagaa
     4141 tcgctgtaat ggataagggt aaagttcttc aaattggtgt gccaatagaa gtttacaaaa
     4201 gcccattgaa tgattttgta gcaaactttg taggtacttc aaactgtatt ggaaaagaag
     4261 attatgttaa ttttaatatt gaaaaagaga caaagcctta tatttataaa agacctgaag
     4321 aaatgatatt attgcaaaat acaaatcaaa gtggttttgt aaaagtaaaa gtagaatcta
     4381 agaattttct aggagcaatt ttagaatata cagtatcaaa tgatgggaaa aaatatcaag
     4441 taactgagct taatagactg aataatagca ataaattaaa cgtaggggat atggcatatt
     4501 taggtgtgct acaaggtgac taatatgatt aaaaaaataa accgtagtaa atttacatct
     4561 aaaaatgtta tttttattgt tttatacagt atattatttt ggattttgat tggatatatt
     4621 atcataccaa taggcaatac ttttgtacaa gcattccaag gtaaaggtgg atattctttt
     4681 gaagttttta aagaatattt ttcaaatact aataatttaa gggtagtaag caatactata
     4741 attttgggat tgggttctgt gttcgtatgt ggaattatgg gaatatcact tgccttatat
     4801 atgacatttg tttgtgataa acataagaaa atcatacata ttcttttact aagtccaatg
     4861 atgattcctg gagtaattat ggtaattgca tgtattcaga tttatggaga aagtggaata
     4921 gttacaaaat ctatagaaat tttactcccc tttatcaaaa aaccttatga ttttagtgga
     4981 ttaaagggaa ttttatttgt ggttgcttat actcagtatg tatattttta tttaaatgta
     5041 tatgttgcct taaaatatgt ggattactca acagttgaag ctgcaaaagg aatgggagca
     5101 tcaaagctaa gagtattttt tgatgtaata tggcctgtta ttacacctgc tgttataact
     5161 tctactatta taacttttgc atcagggatt agttctttct cagctccaaa tcttataggt
     5221 ggtggtttta aagttttaag cactcaaatt gttaggtcta aggctaataa ccatatggat
     5281 atagcttcag ttcaagtaat tactttatta ctaatgggga ttttatttat gattttatta
     5341 cagtattatg gcaaaaaata tggagtagtg ccatccttaa aatctcaaag ttataaagtt
     5401 cataatagaa aaaaagactt atttacattt gtatgtaagg ttattattga aatacaaatt
     5461 attatgatta tgcttcctat agttactatt gtgtatttat cctttgtaag cacacattca
     5521 attatgacaa ccatatttcc tagagacttt acactggaaa actatcagat gattttccaa
     5581 aaatcacgtg tattacagcc tatattaaat agtttcaaaa tgtctttaat ggcagtagca
     5641 gtaggattga taattactgt accagtatca tatttagtaa ttaaaaagga tactgcatat
     5701 aatcgactag caaaatttat tataatgtta ccttggagta tgcctgcaag cgccattgca
     5761 gttaacctaa ttaatacatt taatcaaaaa agtatatttg cttttaatga atccttaata
     5821 ggaggttttt atattgtacc tatagcatat acaatttatg ctctaccatt attattttca
     5881 tccaatgagg ttgctatgaa gtcaataaat ttaggactag aagaatcatc aaggagttta
     5941 ggtgctggaa ggataaaaac cttttataat gtaatagttc caaatatgat gccaggaata
     6001 atttcaggag ctatattagt attcattcgt acaattgggg aatatacaat gtcagccttg
     6061 ctttatggtg tttataatag acctatttca atttctattg taactaatat gcaagaattt
     6121 aagataggag tatctatggc ttatggtgta ttagtaattg gtatatgcta tatagcatta
     6181 ggaataattt ttaaattaga tagaaaaaga tttacataag ataacaaagg ctaatttata
     6241 aattggtgct tagaaaacca aatcacttta ttagctaaag atgaatttga agggtgacac
     6301 tttggtgttt tatgtaaagt ttatggaagt aaaacattaa ataatcgtga gtatgattcc
     6361 tataaatcgt atatactatt gaaatccaag aaaaggtatt ggctaaatag ctaatatctt
     6421 tttaattttt tgactcccat atagcattaa ctattacatg agtatcttta aatttatagt
     6481 taaagtatct taattgttta ttataaaaat ataggatggc taaagcacat taaattaaaa
     6541 cacttaaaaa gagaagatgt acatgagaaa tataattaac tatgatacag acttctaaaa
     6601 attatttagt gaaaaggaac ttagagtgta taataaaagt tccttttcaa taaggaaaac
     6661 gattgttatt ttctgagctt cattaccctg atattattta aatataaaca acaacaaaat
     6721 taacaagaag taaattcaac ctaaaattta aaatatcggg aggtacaata tgttaatcaa
     6781 tatgaaggaa atgctaaaag tggcacaaga aaatcaattt gctgtaccag catttaatat
     6841 aggaagtggg caaatactta aagcagtagt acagggtgct aacgaaaaaa atgcaccagt
     6901 aatacttgca atacacccaa atgagctaag ttttctaggc gatagctttg tagttagttg
     6961 catagaagaa gcaaataaga gtaaagtacc tatggtaata cacttagatc atggggaaaa
     7021 taaagaacaa atattaaggg caataagatg tggatttaca tcagtaatga ttgatggctc
     7081 acatttacca tatgaagaga atgtagctat atctagggaa atagtagaaa tagcaaaagg
     7141 attaaatgtg tccgtagaag gagaacttgg aacaatagga acaacaggaa catcaagtga
     7201 aggtggtact gatgaaataa tatatacaga ttcaaaatta gctaaggatt ttgttgaaaa
     7261 aacaggagta gacacactag ctattgcaat tggtacagca catggtatat atccaaaagg
     7321 ttttaaacca gagcttaaac tagatttatt aaaagaaata agagaagtag tagatatacc
     7381 tttagttctt catggtggtt catcaaatcc agatgaagaa atagcacaag cagttaaact
     7441 aggagtttgt aaaataaata tttcgagtga tgttaagtcg gcatattata agaagtgcag
     7501 agaactatta gaacaaaacc caagcttata tgagccagat actatatatc caccatgtat
     7561 aaaagctgca agagaagtta tagagtttaa aatgaatcta ttcaatgcta tagataagtt
     7621 aaagtgcttt tataaaaata aaaattaatt atgaggtaac aaattaaaag gaggggagaa
     7681 tttatgatag gtaaaatact acgaagcgat tgtattgtac tagataaaca attacaaaca
     7741 aaaaaagatg taattaatta tatggcaaat catttgtacc atggaaatta tataaataat
     7801 aaagaaaagt ttatagctga tattaataaa cgtgaaaaag aatcatcaac taatatgggt
     7861 aatttaatag ctatacctca tgctaagtca aatgtagtaa atcaacctgt aatgatgtat
     7921 ttaagaacag atagagagat tacagataat gatggtcaaa aacttaggtc attttttatg
     7981 atagcagttc cagaatcagg ttcagatgaa catcttaaga ttatatctaa actagctaca
     8041 tatctaatgg aagaaaattt tcttaagcat ctactaagtg cagaaactcc atacgaaata
     8101 ttaagtatat ttaataataa agctgcagaa tcaggagatt ttagtaataa tcaaaataaa
     8161 ggtcttatag ttgggataac aggatgtcca acaggtatag ctcatacttt tatggcaaaa
     8221 aaagcattag aggaagcagg gcaagaactt ggatatgaag ttaaaataga aactcatgga
     8281 gctataggtg tagaaaataa gttgactaaa gaagatataa aaagagctga atacgtaata
     8341 atagcttgtg ataaagaagt tcaactgagt agatttatag gaaagaaagt gctacaaaag
     8401 aaagtagtag atgctaagaa tgtttcatta gctaaaaatt taattataaa cgctgaaaat
     8461 ggaatggcta aagaaattaa accaggtgga aagactttag aagattcaaa agaaatgctt
     8521 caagggtcag agacaaaggg tgtttataag cacttaatga gtggtgtatc tgaaatgatt
     8581 ccatttgtag tgataggtgg aattggtata gccatagcat ttatgtttgg tatatttgca
     8641 tcagacccaa ctcatgaaac atacaaccct atagctggat tctttggaca acttggaggc
     8701 gattcagcat ttaagttatt tataccgata cttgctggtt ttatagctaa atcaatagca
     8761 ggaagacaag gatttcctcc tgctatgata gctggtttta tggcagttat tggaggttca
     8821 gggtttatag gtggtatggt agctggtttt gcaacaggat atatagcttt aggagtaatg
     8881 aagataacag aaaatttacc agatgtatta aaaggagtaa atgctgtatt gatatgtcca
     8941 cttttgattg catttacgag tggagctata atgttctttg tagttaatac gccagcatca
     9001 tggttaaatg aagctttaca atcttggtta caaagtatgt ctggaactaa taaagtatta
     9061 ttaggtgcaa tattagcagg gatgatggca tcagatatgg gaggacctat taataaaaca
     9121 gcttcagctt ttggattggc tatgttttca agtaatatat ttgagccatc agcagcatta
     9181 atggtaggag gaatggtacc tccattagcg atatcattag ctatgttaat attcaaaaat
     9241 aagtttacta aagaagaaag aaagtcatct ctaccaactg ctataatggg ggcatctttc
     9301 ataacagaag gcgcaatacc gtttgcagct gcagacccat taagaatgat tccagcaaat
     9361 ataattggtt cttgtatagg tggggcaatc tgtatgagtt tagatataac tttaatggca
     9421 ccacatgggg gaatatttgt aataccattt gcatgtagtg caccagttgc atatatagct
     9481 tgtatattga ttggtacagt aataacagca ttgattatag gggtagcaaa gcctactata
     9541 agaagtgaaa acagtgaagt tgaattactt ggtgcttaaa ttataaataa tagtaaaaat
     9601 aagtaatgga ataacaacaa gtatatagaa taataataag gatatttaga atcagttaac
     9661 tacttctaaa tatccttaat ttttattttg aaattatatt atctattata tcaatttagt
     9721 aaggtgtatc ataaattctt ttatgataca gaagtatctt agtaatttta catagttagt
     9781 aaatatgtta aaatagtact taatataaac atagaaatat ataaacatag gtagggaggg
     9841 ctaagtatgc ttgtgtcgag gcaaattgaa tttcttaaat tgatactaaa cgaaaataaa
     9901 tatagaccta taaaatactt taaagataaa ttaaatgtat ctgataaaac tttacaaaaa
     9961 gatttgaaga tgattgagaa atatttagaa acttttaata taaaaattga tataaaaaga
    10021 ggctatggaa tattaataga agatttagca aagaagaaca tagacttaat taatagttta
    10081 aatatacaat cgaaggaaaa taaatcttta tcagtagagc agagaagaat ggagatttta
    10141 aagtatttat tactaaattc aaataatata acttcaataa atcagttagc agataaatat
    10201 tatgtaagta aaacttctat agtaaatgat tttaaatata tagaggcatg gataaaagaa
    10261 tacaatttaa aacttaataa aactttagaa ggaactaaga ttataggtaa agaagtggat
    10321 ataaggaaat cgattgccaa aatgatggat gatttattag atgaaaatta tgaacaacat
    10381 gatatatctg agttaacaag attagattct gctacattta gtgcattgat aaatttattt
    10441 gatattgatt ctattatatt tgtagaaact ataattacag aattagaata caatttagga
    10501 tatactataa atcagcccta ctatataaat ttaattaccc atatacttat atgtttaaag
    10561 agaatcgaag aaggtaatca aatagaatct caggaagaaa gagaaatttc tgtagataat
    10621 ttggacgagt tagtatataa aaatgtcact acacttatag ataaaataga aaatagatac
    10681 agagtaaaaa taacgaaaga tgaaattatg tacatatata tttttttggt atcatctgga
    10741 tttagtagcg aaaataatag tagttataat gatgaatcag atatattaag tgaaagtgaa
    10801 aaagtttcat ctataatgat agagaatatg tcacaatttt taaatataaa tctcaaaaat
    10861 gatgacttac tacaaaaaag tctagcatct catgtaaggc ctatgttaaa cagattaaga
    10921 tatgatatcc aaataaaaaa cccgttgtta ggagagatac aagagcgatt ttcagatgtg
    10981 ctgggattat gtatgatgac tataaatatt atgacagagt atgataatct gaagaatatt
    11041 agtatagatg aagtttcgta tcttgccaca tattttcaag cggcaataga aagaaatatg
    11101 agtagtaaaa gagtaatagt agtttgtcat acaggatatg gaacatctca gctccttgca
    11161 gccagattaa aaagagaatt tcctgaatgg actatagttg atatagttcc tatgcatcag
    11221 ttgagtaagc gaaatttaga tgatgtagac tttgtaatat ctactgtaaa cttagatttg
    11281 aaagataaat tacatatagt tgtatcagta ctattaatgg aaagtgatat taataatata
    11341 aaaaatgctt tactttctaa acctaaaaaa agtatgaact tagatacaaa cttattagga
    11401 atgtatttag aagataatat atattttaat tttgatgtag ctcagatgga aagtttaata
    11461 ggtgtgaatt tagggagtaa atatgaatct atttcattag gaaaagattt gaatatatat
    11521 atacataagc aaagtaaatt aaataaaggg ataatgaata tcaataatgt tactagaacg
    11581 attgatattt atttagaaat tagtaatcaa agttttttaa agaatatttt aatggaaata
    11641 tcaaatttat ataggcaaaa gaactatata aactatataa tagattgcaa gagcaaagag
    11701 gatattaaaa tattatttaa gaataatcta ttggagaaaa caaatggaaa ttagagaaat
    11761 attaacagaa gaaagcatta ttttagattt gaaatcaact acaaaagaag gcataataca
    11821 ggaattagta gataaattat acgatacagg aagtataact ttaaaaagta agtttataaa
    11881 ggcattaaaa catagagaaa aaataggctc tactagtata ggttatcaaa tggccatgcc
    11941 acatggaaaa acaaaatatg taaagaaggc atccatagta ataggtaaat ctaaaaaagg
    12001 tgtagattgg ccaagtatag atggcttacc tgtaaaggta ataattatgt ttgctatgcc
    12061 acaatctgga ggcaatgaag atatgatatt aatatctaaa atagcttaca ttttaggtgg
    12121 taaaaatata agtgaaagac ttttaaatgt acatacaaaa aaagaaatat tagatatatt
    12181 ttatgaagtg taggtattgt ataaattata aatttgaggt ttttaaaata aaatgtgtat
    12241 tcttttaaat agtatagatt aatttttaga cactagagag gagaggatat ctatggcaat
    12301 atatttagaa atgcctaaat taatacatga atttcctttt cgtagtctat caaatgaagg
    12361 agaagtatta acaacaccac actggcataa ggaaatagaa atattactta taacagaagg
    12421 tgtagtaaat ctatttataa atgacaagcc tatgcaatta aaaaaaggcg agatagtgat
    12481 aataaaagga ggagatattc attatatact gccatcaccg ggaagtgaaa gattagtatt
    12541 tcaatttgat atatcttttt ttaatgattt aattttatta aatgaggaaa aagaaagctt
    12601 aattaattta ttcagtagta taaaaaaatg tagtagagat tggtcagcaa aaataagaaa
    12661 gtcagttttc gatatattga tagatatcta caatgaagac ctatacaaaa tagaaggata
    12721 tagttatgca ataaaaggta aaatgtataa tttaattcct atattatata gacagattcc
    12781 tagagaaatt gaagaaaata cagtagaata tgaaataaac tctaaagaga tattagagag
    12841 attgaacaat atattcaaat atgtagaaaa aaattataaa caacctataa aattagaaga
    12901 tgtgtcttat gaagtaggat ttagtgtata ttattttact aaatttttca aaaaaaatac
    12961 aggaaaaaca tttataactt ttttaaatga atatagatta gaaaaagcaa agtggatatt
    13021 acttaataac aatatatcca ttgtagattt ggttgaagaa gttggttttg gaagtacaaa
    13081 aactttttac agagtattta aagaaaaaat tggagtctct ccattagaat ttaaaaaaag
    13141 atataatagc ttggagaaaa aagggggtgc ctcaaaatga acttagttca tgaggcactt
    13201 tcttttatat gaaatcaaat atattttcag tatttcaaca atgaaaattc cattcattta
    13261 taaagtctaa attttagttc tcaatacaat ctctatttcg aaataaatcc ttttatatat
    13321 ataaatatac agcaatatat gagaataaga taacaataat gaggatataa aaatgtattt
    13381 gctatatata taattatata catagatatc taatattgat atttaatatg aaagtattga
    13441 aaatagacaa tattaattat agtgagtaaa aaatataatt ggatttttaa attgaattat
    13501 ttaaaaggta tagtatgaaa aaaatcttat aactaaatat gagatagaaa ttataaaact
    13561 ttatactgtg aaagaggtag atgcatgaaa ttagggaaag attttttatg gggaggggca
    13621 atttcagcca atcaaagtga aggattttat ttagatggag gaaaaggttt atctcagttt
    13681 gataaactcc cattaaatga taagagatta aaagatgtat gtttagatga ggataatatt
    13741 ttaaatgaaa ataatcaata ctatccaagt catataggga ttaaattttg tgaaaattac
    13801 aaagaagata tagcaatgtt agcagaaata ggttttaaat gttttagatt ttctatatct
    13861 tggtcaagaa tattcccaac aggagaagaa agtgaaccaa atgaaaaggg attacaactt
    13921 tatgataata taataaaaga attaaaaaaa tataatatag agccattaat aacgataagc
    13981 cactttgata taccttattc tctagttgaa aaatattctg ggtgggctga taggagagtg
    14041 gttaatttat atgtaaaata tgctgaaatt ttgatgaaaa gatataaaga tgatgtcaaa
    14101 tactggattc cttttaacga aatgaatatg ataattcata ttccatatat aggtgctggg
    14161 ttaactttta tagctgatga aaatcaactt cagaaaaaat atcaagcagc acaccatcaa
    14221 ttattagcta atgcaaaaac tatagaaata ggaaggtata taaataaaga atttaagttt
    14281 gggtgtatgt tagctgctgg aaaaacatat ccatacacat gtaaaccaga agatgtattt
    14341 gcaactttag agcatgaaag gcataatttg tttttttctg atgttcaagt taggggtgtg
    14401 tatccaagct ttatgaatta ttatttaaaa tcaaatcaaa taaaattaat aataaaagag
    14461 gaagatttat caacacttaa ggataatact gtagattttg tatcttttag ctattattct
    14521 agtgcttgta gttcagctag agctgaaggt ttagaaaaat caaaggcaaa cggaccagaa
    14581 accataaaaa atccatatct tccaaaatca aatagtgtat ggcaaaacga ccctgtaggg
    14641 ttaagaataa ctatgaatca gttatatgaa aggtatcagt tacctttatt tatagtagaa
    14701 aatggactgg gtactcaaga tattgtaaat gaagatggaa aaataattga tgattatcgt
    14761 atagattaca tgaaagaaca tattaaaaat atgattcttg gagtaaatga agatggaata
    14821 gagcttatag gatatcttat gtggggatgt atagatgtaa ccagtgtaag tgaaggaaag
    14881 atgacaaaac gatatggcat aattcatgtt gattctgatg actatggaag aggctcattt
    14941 aaaagaacta aaaaacaaag tgctttttgg tataaaaaag taatagaaac agatggagaa
    15001 attttattta atgatatgga aactcatgca taattcattt taagaaagga gtgttattat
    15061 gagtttaata tttactacta aggaccaact tatagagcaa gggtttagta aggatacaat
    15121 aaaatccaat gaaacgttgt ttacactagc taatggacat ttaggagtta gagggaatat
    15181 tgaagagtcc gattttaata atgagtatgt agataatatg ggtacatatg taaatggatt
    15241 ttatgaagaa tctccaatta catatggaga aagcgcttat ggctatgcaa aacaaaatca
    15301 gactatctgt aagttaccta atgctcatat agtaaatttc tctattgaag gagattgctt
    15361 tgatttatca aaaggtatta caagtgaaca tactaggata ctagatttaa atgaaggaat
    15421 tgttaaacgt tcatttatat gggaaaactc tcgtggtaga aaagttaaag tatctataga
    15481 aagattagta tcacaagata tagaagaatt aatgatgatt tcatatacaa taacaccatt
    15541 gaattttaat ggaaaaataa aatttaaaaa taaaatggaa aatagtatgg agaagtttaa
    15601 ttgtgaaaaa gtagatgacc caagagtggc atctataaat aagaagcaat ttaaaattaa
    15661 tgtaaaaaaa tataacaata agtattttat gaatttagtt acagaaagaa gtagattagg
    15721 gttatggtgt gggtttgatt catatattac ttgtggaaat attacaaatg agacctataa
    15781 tgtttcagat gaatgtattg agcgagaaat agaagtctat gccaaaaaag atgaaaggtt
    15841 aaatttagaa attgttgtag gctatagtag agtatattct gatttaaatc taaatgaaga
    15901 acgtttaaaa tcattagaat gtctggagtc gttaaaatgt ttagaatcag aagcacattt
    15961 aaaatcatta gaattggttt taagagaagc atgtaatatt ggatttgata aagtcaaaaa
    16021 tattcagaga aagaaaatga atgagttttg gacaacctct gatgtagaga ttgaaggcga
    16081 taaaactctt caactgggta taaaaaccaa tttatttcat atttatcaat cagcaggaag
    16141 agatggaaag actaatatat cagcaaaagg tcttacaggt gatggctatg aggggcatta
    16201 tttctgggat acagaaatgt atatattacc attttttata tatactcaac catccattgc
    16261 tagagcttta ttagattata gatactcaat cttagatgca tcaagagaaa gagctaggga
    16321 acttggaata aataaaggtt gcttatattc atggagaact ataaatggta atgaatgcag
    16381 tgcatattat ccagcaggaa cagcacagtt tcatataaat gcagatatag cttatggtat
    16441 tagaacgtat tttgaagcaa ctaatgatga agaatttatg atgcaaaatg gcttagaaat
    16501 tcttatagaa acagcaagat tttgggttga atttggagat tatatagaat taaaaaataa
    16561 taaattctgt ataaatggag taacaggacc agatgaatat acagcaatag taaacaataa
    16621 ttactacact aatttaatgg ctaaatataa tttaatatat gcagtacaga tggtagataa
    16681 atttaagcat aatttatttt caaatgaagt atttagaaaa gttaaattac aagaatatga
    16741 agtaaaagac tggataaatg ccgctcaaaa tatttattta ccatatgatg aagagaagaa
    16801 aataagcaaa caagacgatt catttttaga aaaatcagta tgggatttta aaaatacacc
    16861 aaaagaaaaa tatccattac ttctcaatta tcatccatta acaatatata gatatcaagt
    16921 aaataaacaa gcagatactg tactttcagc gttgcttttc cctgatgagt ttagcttaga
    16981 acaaaagaag agagattttg aatactatga aaaaataact actcatgatt catctttgtc
    17041 aaggtcaata tttggaatga tggcaagtga aattggaaat catgaaaaag cttataacta
    17101 ttttatggac acagcattaa tggatattaa taacctacaa aaaaatacaa gtgatggaat
    17161 acatgctgct aatatgggtg gaacatggat gagtttagta tatggatttg caggaatgaa
    17221 aataaaaaag gataaattaa gttttgaacc aaggatacct aaacactgga aaaatattaa
    17281 atttaatata ttatttagag ataatttagt ttctgtaaat atagaagaat ataaatctta
    17341 ttataagtta atcaaaggta aaaacataag catattacat gataataaag aaataaaatt
    17401 atcaaagaac ataatagaag ttaataattg ttttaaaggg agtaattaat atgaaagcag
    17461 tcttatttga tcttgatgga gttataacag atactgctaa gtatcatttt gaagcttggc
    17521 atgagatagc aaaatcgcta gatatagaaa tagatgaaac ttttaatgaa aaattaaaag
    17581 gcataagcag ggaagaatca ttagataaaa tattgttatc tgctaataaa gtagatagtt
    17641 atactaaaga ggaaaagtta aaggtgtgtg aagaaaaaaa taacatttat ataaagttaa
    17701 taaactcatt aacatataaa gatatattac cagggataaa tgagcttata aaagaactta
    17761 aaaaaaataa tgtgaaattg ggaatagcat ctgctagtaa gaatgcacct aggatattag
    17821 aatctttggg aataaaagat gaatttgatt gtatagtaaa tccatcctta ttagaaaggg
    17881 gaaaaccata tcctgatatt tttattgaag ggagcaagat gctaaattta aaacaatatg
    17941 attgtgtagg aatagaagat tcttattcag gtattaaagc tataaatgct gccaatatga
    18001 catcaattgg tgtggggaaa aaagaagtac taggaaaatg tgaccatatt gtagaagata
    18061 ctagcttatt aaattacaat acactattac aagcttggag taaaaagatt aatttggatt
    18121 aaaattatat taaaaattat tttaaaagag aggataaggt atgaagaata aatatgaaga
    18181 agtagctcaa ttgataattg atggacttgg aggaaaggat aatataattg acttaactca
    18241 ttgtatgaca cgattaaggt tcatcttaaa agatgaaaag aaagtcaatg cagataaatt
    18301 aaaatctata gataaggtag ttggagtaaa ctcaacttca actcaatatc aggtcataat
    18361 aggtaatgag gtaggtgttg tatttaatgc aataataaat aaggggataa ataccacagg
    18421 agatattaaa agcgatataa aaaatgaaaa taaaaaaaat agtattttaa ataatataat
    18481 agatacaata actggttgta tgacacctat gataccagcc cttacagcat ctgggatgat
    18541 aaaagttata ttatctcttt gtaccacttt taattggcta tcatcaaatt caagtacata
    18601 tagagtatta gatataatag gagatgtagc attttacttt atgcctataa tacttgcaat
    18661 aaatgcagct aaaaaattta atgttaatac atcaatagca gttatagtag taggtgtgtt
    18721 tttacatcca aatttctctg catgggtatc aagtggggac cctatatcat ttataggagt
    18781 accaattcaa ggtgttatat atgcagcatc tgttatccct gcacttttaa cagtatggat
    18841 gatgtcatat atagaaaaat tcattaataa attaacacct agcatgttaa aaacaatttt
    18901 aaatccaact ttagtattat taataagtgc gccaatagca ttaatagtaa ttggaccaat
    18961 aggaaatttc ttaggagagg gattagctag tataataaat ttactacaag gaaggttagg
    19021 atttataatg gtttgtttat tagcagctgc aatgccattt atcgttatga cagggatgca
    19081 tcatgcattg actcctatat tcatatcagc atttgcaact acaggacaag aatcgctaat
    19141 attaatatct caagtttgtg ctaatttagc gcaaggtggc gcgacattag ctgtagcatt
    19201 aagaagcaaa gatagtaaga tgaaacagtt agcatcagct gcatggatat cagctatcat
    19261 gggtataact gaaccagctc tttatggagt tacattgaaa cttaaaaaac ctgctatgtt
    19321 agcatctata ggagcaggaa tcgcagggtt atttgcagga attactcatg tgactttata
    19381 tgttccacaa aatagtataa tggcagtact tggattatca ggggaaaagg gaatttcaaa
    19441 tatagtaaat ggacttatag ttatatttat agcattggtt gctccattta ttatgacata
    19501 tgtttttgga gttgaaaaaa atgttaagga aagtacagat aatgtagtag aggaagtata
    19561 atattaacta tttacattaa tagaattttt attattattg actataactt gttttgtatt
    19621 tatattgatg caaagaaata agttaatatt aatgataata attattataa ctgacaaaaa
    19681 gcaacacaaa agattgatga agtatatgga aataaaaagt tgacaaaata tgtctgatgt
    19741 tttatcatat taattggaat taggtagtaa ttgtttaagt acaagctaag cttattcatt
    19801 aataatagca gtatcctagg aaggagtatg cccattatta tggataagct ttttttatgc
    19861 ttaaaaggag gataatcatg ataattaata agatattaaa caataatgtt gtaatcacat
    19921 tagatgataa tgatgaagaa gttatcgtta tgggtaaagg aattggttat caaaaatcaa
    19981 aaggcaatct aatagataaa actaaagtaa ataaagtatt tagaatatca aataaagaaa
    20041 tatcaaacaa actacaagaa ttactcaata atattcctat agaacacatg aaattatcaa
    20101 gtgaaattat agaatacgca caaataaagt taaacaagaa gctaaatgaa agtatttaca
    20161 tatccttaag tgaccataca tattctgcaa ttcaaagaat gaaagaagga ataaatgtaa
    20221 aaaatgcaat tctttgggaa attaaaagat tttataaaga agagtttgaa attggaatga
    20281 aagcactaga tattattgaa aacaaaacag gtataaaatt accagaagat gaggctggat
    20341 ttatagcctt tcatatagtg aatgctcagt taagtgaagg tcatactttg gcatctgata
    20401 ttacgaagtt aatacaagaa gtacttagca tagttaggta tcattttagg atagaatttc
    20461 atgaagaatc agtgttttat tatagattta taatgcattt aaagtttttt gcgcaaagat
    20521 tgcttttaga tagtgcacat gaaggtgaga cagacaaaga attactaagc attattaaat
    20581 ctaaatataa taaagagttt gaatgtgtag ttaaaattaa aaatttcatt aaaaaacaat
    20641 ataactacat attaacagat gatgagctta tatatttaac aatacattta gctaaagtag
    20701 ttaaagactc aaatatgtaa gtgaaataat ttttcagtag tatattaaga atgattgaaa
    20761 ggagtgatac taacaaaatt atacctatgg ttggatggta acattgagtt ggccaaacct
    20821 tcaaatttca aaaaattaat ttaaacgaaa tatggaggag aaaaatggga aaatacaaac
    20881 aattagcaca agagatagta aacaatgttg gtggaaaaga aaatattagt ggattagtac
    20941 actgtattac aagattacgt tttactttaa aagatgaaag tatagcaaat gacaatgtat
    21001 taaaaaacat ggaaggtatt gttactgtaa tgaaaagtgg aggacaatat caagtagtta
    21061 taggaaatca tgttgaagct gtctataaag atgtagttga aataattggt ttagatgatt
    21121 caagtacaag ttcagaaact aaaaaatcag gtaatatact agataaaggt atagatataa
    21181 tatcaggaat ttttcagcca gtgttaggaa taatggcagc ttgtggtatg ctgaaaggat
    21241 ttaatgcatt atttgtagca atgggattat atagtgatgt atctggtggt tatatggtaa
    21301 taaatgcagc aggagatgca ttatttacat tccttccttt gttcttagga tatacttcag
    21361 ctaagaagtt tggattaaaa ccaatgttgg gattagcact aggagctgca gtatgttatc
    21421 ctgctataca aggtagcagt atatcagcag gagcagatgt gatgtacact ttatttaaag
    21481 gaacaatgtt tgcatcacca gtatatattg atttctttgg actaccaatt gtatcaattg
    21541 attatactgg aacagtcgta ccaattatat ttatagtgta ttttgcttct aggtgtgaaa
    21601 aattatttaa taagtttgtt ccagatttag ttaagttttt ctttgtgcca atgcttacat
    21661 tattaataac agtgcctgtt gcacttatag ttataggtcc tatcgcaaca tttggttcta
    21721 cacttatttc acaaattgta atttcaataa gagattttag tccactattg gcaggtgcta
    21781 tagtagggtt tacttggcaa attcttgtta tttttggtat gcactgggga tttattcctg
    21841 tatatataaa caatgttatg actttaggat atgataatgt tatgatgcca ttctttgctt
    21901 gtacatttgc aagttcggca gttgtgttgg caatattttt caagactaaa gataaaaaac
    21961 taaaagagat gacaatacca aactttatat ctggtatatt tggagttaca gagccagcaa
    22021 tttatggaat attattgcct ttaaagaaac catttataat aagttgtatt gctagtggga
    22081 ttggaggcgc tttctacgga ttctttaatc ttagaaaatt tataactgga ggtatgggaa
    22141 tatttgaact tccagcaatg attgaaccag atgggggaat gggcaactta atagtagctc
    22201 tttctggtat agctatatca atggtcgtag cttttgtatt aactatgatt ttatataaag
    22261 atgagaaagt agaagagtct aaaaaagtaa gaaataaggg gaaagaagaa ataaaagaag
    22321 tcaagtctac taaattagaa agagaaatag tagcaagtcc tataaaaggt gaagtattaa
    22381 aattaagtga tatagaggat gcagcatttg catctggtgt actaggtcaa ggggtggcaa
    22441 taatacctag tgatggaaaa gtagttgctc cagtagatgg tgtagttaca accttatttc
    22501 ctagtctaca tgctataggg attctttctg atacaggtgt tgaagtgctt attcatattg
    22561 gattaaatac tattcaatta gaagggagag gttttgaggc atgtataaaa caaggtgata
    22621 gaattacaaa aggtcaaact atattaaact ttgatgttga tgcaataaaa gagttggggt
    22681 atagtactgt aactccaatt gttattacaa attcatcaca atttttagat gtagtagaaa
    22741 cagaaagtaa aaatattgaa ttagaagata atctaattac agtcttattt tagtaaaaat
    22801 tgaggcatta gcataagcta gtgctttggt tataatttaa ggaggctttg actatgagtt
    22861 ttaaggatga ttttttatgg ggaggagcta ctgcagccaa tcaatgtgaa ggtggataca
    22921 atgaagacga aagaggattg gcaaatgtag atgtatgtcc aacaggaaaa gatagaacag
    22981 cagttattac agggaaacta aaaatgtttg attttgatga tgagcattat tatccagcta
    23041 aaactggtat tgatatgtat cataattata aagaagatat aaagttattt gctgaaatgg
    23101 gatttaaagt atatagaatg agcattgcat ggagtcgaat atttccaaaa ggtgatgaag
    23161 aaacaccaaa tgaaaaggga cttcaatttt atgaaaatat atttaaggag tgtaaaaaat
    23221 ataaaataga gcctttagtc actattacac actttgattg tccaatgcat ttagttaaaa
    23281 agtatggtgc atggcgtaat cgaaaaatga taggtttcta tgagaaatta tgcaatgtta
    23341 tctttagaag atatagagga ttagtgaagt actggctaac ctttaatgaa ataaatatga
    23401 ttttacatgc tccatttatg ggtgctggta tttgctttga agaaggtgaa gatgttgaaa
    23461 agataaaata tcaagctgct catcatgagc ttgtagctag tgcaattgca actaaaatag
    23521 cacatgaagt tgattcaaat aacttagttg gatgtatgtt tgcagctgga agtgtatatc
    23581 cttatagttg taatcctaat gatgtatggg aagctacaaa gttagataga gaaaattact
    23641 tctttgtgga tgtacaatct aaaggtaaat atcctaacta tgcgttaaag tatatggagc
    23701 aaaaaggtat tacaccagaa atggaacctg gtgatattga attattgaat aaatatacag
    23761 tagattttat ttcattttca tattacaata gccgttgtgt tagaactgat gaaaatgcag
    23821 atgatatggc tgaaggaaac atatttacat ctgctaaaaa tccatatctt tcatacagtc
    23881 aatggggatg gccaattgac ccattaggtt tgagaattac actaaatcat gtatatgata
    23941 gatatgaaaa gccactattt attgtagaaa atggattagg tgctaaagat atagctgatg
    24001 aaaatggtta tgtagaagat gattacagaa ttaattatct aagagaacat ataaaagcta
    24061 tgcatgatgc agtttctata gatggggtcg atttattagg ttatactaca tgggcaccta
    24121 ttgatttagt aagtgctggt acaggtgaaa tggaaaagcg ttatggattt atatatgtag
    24181 atagagataa tagtggtaat ggaagtctaa gaagaatgaa aaagaaaagt tttgaatggt
    24241 ataagaaagt aattgcttca aatggtgaag atttatatta aacaagaaat ctatgttaaa
    24301 taagaaattt gtattaaata agaagagtat aaaaattaat aataagtata atttttaact
    24361 tttttaacta tagaaaatca atacagaatt attttacatt gtatttatgc aatagtatga
    24421 ttcacaaata aaattttaga aaataaaatt gttaagaagt atgggaagta agtgcaattt
    24481 actttgtaat aaaagatgaa aaaggcgagc ttaggtttta agcttgtctt ttttattatg
    24541 tataaactat gatactttaa aattctatga aaaatttatt taatagagaa attaaaaatt
    24601 taatggtaat caaattcttt accaatgaat ataaaaaatg tctacgtgtt ataatagttt
    24661 aaactaataa acgaaaggta gatgaatgaa tatgaaatac caacatataa tagaagttga
    24721 caaagaactt tgcattggat gtgggctttg taaaaatgat tgtccagtaa acaatataat
    24781 tatagaaaat aaaaagtcag taattaaaaa acaagattgt ctaatgtgtg gacattgtgc
    24841 agcaatatgt cccacaaaag ctattacctt gacaggattt gatgaacctc ctatagaact
    24901 tacaaataag ccaaagctag attcagatga attactaatg gcaattaaat ctagaagaag
    24961 tatacgtaaa tttaaagaca aagaagtttc ttcagaaatt gtaaagcaaa ttattgaggc
    25021 aggtaggtat acccctagtg ctaaaaattc acaagatgta tcatatattg ttttagacaa
    25081 taaaaagtca atttatgaaa acgaagcagt gaaatttttt agaaaaataa aacctattgc
    25141 aaatatagct attaaatatt caaaagaagt tgagattgac gataactttt tctttaagca
    25201 tgctcctatt gcaataatga taattacaaa agataagatt agtggttcac ttgcagcatc
    25261 aaatatggca ttaatggcag agtcttatgg tctaggtgtt ttatttagtg gatatttttc
    25321 agatgtagca aataattcgc caaaattaaa aaagctttta ggtctaaaac gcagtaatca
    25381 tgttcttaca acattagtaa ttggataccc agatgttaaa tatagacgta ctgcacagaa
    25441 agaagtagca actgttagat acttataaga gagggtggat tttatggaaa aagaatgtca
    25501 acaaagaatg caagaaataa catctaaatt taaagaatgt cgcaaagcta tctctgctat
    25561 gggtgatgag actagacaat tgattttaat tgctctctta gagagtgatt ttaatggtat
    25621 acgagtggga gaaatcacaa aaaaaacaca tctttctcgt cctgctgtat ctcatcatct
    25681 taagatatta aaagaagcag agattgttaa tttgcgtaga gaaggaacga aaaattatta
    25741 ttatcttgat tcaaaaaaat cacaatggaa gtcactaaca gagctaataa atcttatatt
    25801 tgttggaata caacatatta gtgaaaatga tataagaaaa ggtgattaaa ttatacttga
    25861 tttaatacaa aaagattgat ttaggaacta aaaagttcac tttgagacac tatcttttga
    25921 actataccca tcttaggaga cagttaaaat atgtaaaact gtataaaaca atgggtatag
    25981 ttcattttat aatggtttaa atattaagaa tatattattc taaatagtat ctttctctgt
    26041 tttgagtttt atttttatac tcaatagctt cagtggggca accacaaata catgccatac
    26101 aatgagtaca gctatttttc caaactggct ttttgttttt taaattaata ttatttaaag
    26161 gacatagctc tacacatttt ccacagccaa tgcatttatc agtataatga aatcctttgg
    26221 cactaataat tgtcttataa aaaactgtat ttattatacc acttttaatt gtacctccta
    26281 agccactagg agtaatagtt tcaaattcat tttcattgcg tatatcagaa ataatcctat
    26341 atatttgttt atctgcttct ttaatcattt gttttgatgt ttctttgtct gttgtagaaa
    26401 ataaagctat ataattctct ggcattttaa tttctgccat accttttaat tgccattcct
    26461 tgtatttaca tagtttttga atatagttta tggattttgc tgtatctccc ccacaagtca
    26521 ttacaaagta aactctgttg tctcctagaa attttgtttc ttttataaaa tctgtaacca
    26581 ctattggcaa tctccatgca taagtaggac atacgaatat gaatggttta cctgctgata
    26641 ttaattcatc tgttttttcc tcttttataa gttgatttag tgatatcagt tcatcattta
    26701 attcttgtgc aattttacga gccacatatc ggctattacc agtaccgcta aaatataaaa
    26761 tcataataat atctccctaa ctatttttta ttttaactct taatttttta tttattatag
    26821 tactttattg aaatattcta ttcaaatgta tgtaaaattt agtagttaag aaaattataa
    26881 cacattagta atttttatat tcattggtaa agaatttgat taccattaaa tttagtaaca
    26941 aaatatcaac tggattttta tataagaaga gatagtaatt ttcatgtgaa agattgtaaa
    27001 tataaagatg attttgaagg gcaataagaa gataaaattg atagcttaat aaaaaatatt
    27061 taacttttat attaactgtt ttaaatatta taattatcta tcttgaataa tcaaccatag
    27121 aagactgtat acaaagacaa agttgtgtac agtctttttt ataaatttaa tgtctataat
    27181 taaatacact attaaaatca ataagttatt atttcaaagt agtattatcc aaatttttat
    27241 gatggttaat acaatttgtt agccatttta tagcaccttg ttccctgata atagctccat
    27301 caagaactat ataatcacca aaatcattac tatcaagagg aggaacttca ctataacgct
    27361 ccatttgact gtacaagaac gtaattcttt cttggtgttg aagcaattga tgctctagta
    27421 aaataagcct agtatctata tctaaattat tagaaaaata cattctaaga cgaaaaatat
    27481 ccttaggggt tctttccatt ggctcatcct tttcaagcca tttcaagaac tcatctttac
    27541 ctttttctgt gattgtgtat aactttttct ctaatacatc accactaatt ttaatatcat
    27601 atacaataag cttttcattt accaattttt ttagttcagg atatatttga ctatgctttg
    27661 cattccaaaa ctcagctaat tgaaaattga actcctttac gatatcataa ccagtcatag
    27721 gatttctatt taataatcct aaaattgcat actttaaagt tcgcataaat acacctcttt
    27781 ctttaattac atacttatta taacacgata tatttagttt gaattaatta atatagctat
    27841 gttttaataa gaacttttaa attatgagga acaaaaaata aataaaacta tatataaaaa
    27901 tgaaattatg taaatataaa catgtaagta tttacatgtt tatatttaca tatagaaaaa
    27961 attgtgataa tcttaactaa gaaattctat ttaataaatt tatcgaggag gagagtaatg
    28021 aatttaacac aaaaaagatg gattatatta attgcaagtt gtttcatcaa tttatgtatt
    28081 ggttcaatat atgcgtggag tgtatttgca tcacctatgg cagaacattt aaatagtctt
    28141 gctggaacaa cttttacagc tgctagttta gcaattgtat ttacagttac aaactcagtt
    28201 ggacctatta ctatgattac agggggaatg ataaatgata aatttggtcc taaaaaagtt
    28261 gtatttgtag gaggattaat ttttggtata ggtatgatat tggcaggttt ctcaaaagga
    28321 ttgcctatgt tgattttatc atatgggata ataacaggtc ttggggttgg catggtttat
    28381 ggatgtacaa taagtaattc aattaaattt tttccagata aaagtggatt agttggtggt
    28441 ataacaacag cagcttatgg gtttagttca gtaataatgc ctccagttgc taattggata
    28501 attaataaat atggagtaac ttctgcattt aaatcaatag gtttagtatt tcttataatt
    28561 gtatgtgttt cttctttctt tatcaaaaaa tgtccagcag atttcacacc agaaggatgg
    28621 gtaccaccag agaaaaaagg aaaagtaaga gttgttatta ataaaaattg gaaagaaatg
    28681 ctttcaacac ctatatttta tctaatgatt ataatattaa cttgtggagc tttctatgga
    28741 ttaatgtgtg tgtcattagc atcttctcta gcaacaaata tgattgggat gacaagttca
    28801 gattcagcaa tagtggtttc aattttagcc ttgtttaata cattaggccg tattgttgca
    28861 ggatatatat cagataaaat tggtagaata aatactttat caggtgcatt agttatttca
    28921 atttttggtt tattatgttt gttattctca agagaaggag atgtagcaac attctatatt
    28981 ggaataataa ttataggtat atgttttggt tcatttatgg gaattttccc aggatttaca
    29041 gtagataaat ttggacctaa aaataatagt gtaaattatg gaatcatgtt tattggtttt
    29101 gcttttgcag gatatattgg acctgcagtg atgaaaaatg tatattctgt agatggcaat
    29161 tatcaaaggg ctttcattat agctataatt atagctttaa taggttttat attaacattt
    29221 ttatataaat atataaataa aatacaaaat ttaaagatag aaaatttaat aaagggagat
    29281 ataaaaaatg gaaagaaaat atccaaatct atgtaagcca attaaaattg gaaatgtaac
    29341 attcaaaaac agaatgtttt cagcacctat ggggggaaca gacataacag cagattgtac
    29401 aattggctca aaatcaacag ccttttacga attaagagca aaaggtggag caggtgcagt
    29461 tacaattagt gaatgtatgg ttcatccaga aacagatggt tcacatgcat atcatctaga
    29521 cttaaaaata gttgattcct tagcaagttt tacttataca gcagatgcaa ttcgccgtca
    29581 tggagcaata ccaagtgtag aactatctca ttcaggacaa tattcaggaa cttatttagt
    29641 tgacaaagat aagaagcgag gattatcaca atggggagta agtcctagtg ttcgtcctga
    29701 tggattagag ataaaagaac ttacagaaga aataattgct gaaatagtag agtcatatgg
    29761 aaatgttgca gcattggcta agagagcagg gtttgagatg attatgattc atggtggaca
    29821 tggctggcta atcaatcaat tcttatcacc ttactttaat aaaagaactg acaaatatgg
    29881 tggttcttta gaaaacagag ttcgttttgc taaagaagtt ttagatagtg ttcgtaaagc
    29941 agtaggtcca ggatttccaa tagagtttag gatgagtggt tctgaattat ttgagggagg
    30001 gtatgattta gattatggaa cagaaattgc taaattattg gaatcaagag tagatttact
    30061 tcatgtatca gcaggtacat accaaagagg gtttgcagta acacatcctt ctatgttctt
    30121 agaacatgga tgtaacgtat atcttgcaga agaaattaaa aaacatgtaa atattccagt
    30181 tgctactata ggagcattaa atgacccaga aatgatggaa gagataattg ctagtggaaa
    30241 agcagatgtt atttacatgg ctcgtgcatt actagctgac cacgaattac caagaaaagt
    30301 tatgagtaat caagatgaaa aaatagtgaa atgcctaaga tgcttcactt gtatggcaga
    30361 acgtgctacc acttctacaa gacgctgcac tgttaatcca cttattggac gtgaattaga
    30421 tggaactgaa gttgtttcag tattaaaacc taaaaaagta ttaatagcag gtggtggacc
    30481 aggaggattg caagcagcca ttacagctgt aaagcgtgga cataaagtaa tactttgtga
    30541 aaaaactaat gaattaggtg gaattttaaa gggagaacaa gcacttccat ttaagtataa
    30601 aatgtatgaa ttaggaaata cttttggaaa aatagcaaaa gatttaggtg ttgaagtacg
    30661 tttaaataca acagttacaa aagaatatgt tgaaaatgaa aatgtcgatg cattaattat
    30721 agcagtaggg tctgaacctc ttgttccacc aatagagggt cttgatggag aaaatgtagt
    30781 aatagttaat gattattacg tagaaaaaga aaaagtcact gacgaagtaa tagttttagg
    30841 tggaggatta gcaggatgtg aggctgcaat tcacttagct caagaagaga agactgttca
    30901 cttagtagag atgagagcag aacttgcccc agatgcaaat ataaggcatc gtccaatatt
    30961 attagaagaa atagaaagac aaggtatata tgtatataca gaacataagg gtttaagtgt
    31021 aacaacagaa ggtgtagtat gtatgaataa atctgggaat aaaataaata ttccaggaac
    31081 ctcagtaata tgtgcacttg gacaaaaacc aagaagagaa gtggtagaca ctttacttga
    31141 ttctgctcca tatgttgctc aaattggaga ctgtgttcgt gcatctacaa taacaaatgc
    31201 agtataccaa gggcatcatg ctgcgctaga tatatagtta aaaagacgta gatagtattg
    31261 ttttattata aaaaaggagt gtctcaaaat gaacttttta gttcatgaga cactttttta
    31321 catgaaatta aatatatttt tatcatttca acaatgaaaa aagattatct ttattttgag
    31381 acagcacttt tgttattatg attttaattc ttattgcaag ttttcatttg attgatgagt
    31441 aaaacttttt aagatgtaga agtatttaac aatgtaaatg accctaactt aaatacttta
    31501 gcgaaggttg acagatttgt gtttgctttt atctattata acttaaggaa acatgttacc
    31561 tgaacaataa atttatataa taaaagtata taaatatata tatgaatgtt aagattagtt
    31621 gatagaattt aaataataaa tccaatatta taaacatagg aggtatgaaa aatatgatta
    31681 atgagaattt aaaaagtcta agaaaaatcc accagtatac acaagaagaa ttagctgaaa
    31741 aacttaatgt ttctagacaa tcaatagcaa aatgggaaag tggagtttcc caaacaaaag
    31801 gatattactg aaataacctg tgtttacctg cttgtagaga aaagaatatt gctattaagc
    31861 cagagatact acataaatgt gttggtatct ctttttcgtt aatgcaacac agtaaattat
    31921 ttcattttcc taattatttt agactgataa gtaacttttt aaatgaagaa aagaaatgac
    31981 aatacttaaa ggagcatggt atacttaaag gagaatttca ttaaggagga aaaagagatt
    32041 gtttaatcta atagaaatgt tatttcctat aatgtttata ctgattttta taataattgt
    32101 tttcactttt gtgaaaggaa tagctacttg gtttaaaaac aataattctc ctcgcttaac
    32161 tgtgtcagca agaatagtag caaaacgtca gaataccact cataacaatc aaccaaacgc
    32221 tggtgatata tcaggagcac atggatttca tacaatttcc agcacctcgt attatgtgac
    32281 atttcaagta gatagtggtg atagaataga gctgtctgta tctggctcag agtatggagt
    32341 gcttgcagag ggagataaag gacagctgac attccaaggc acaagatatt taacatttga
    32401 tagggaaaat tgaatttaga ggtgcattat gaataagttt ttgagtcaga taaggcgtat
    32461 agataatgaa atatctatta ctaggaaaat tataaataca atatttatac tgtgttttgg
    32521 aatagtttta ggaacttttg caaaattttt agatactaca gcaagcaata ctttaccatt
    32581 tatatttgaa tatttagata ttagtaattt ttttggtcgt tttgctatat ggttattgat
    32641 tgctttgtac attgcaattt atagtcattc ttccataaga gcatcattaa atgtgttagt
    32701 atttttcatt ggaatggttt caagttatta cctatattcg tattttgttg ctggattttt
    32761 tccaaaaaat tatgcaatga tttggcttgg ttttactgtc atttctccat tgttagcttt
    32821 tatttgttgg tatgctaagg ggaaaagcaa aatatctttt atactttcag taataataat
    32881 tgcaatttta tttaatttta catttattta tggttggatt tattttgatg tgtattcaat
    32941 tttagaagtg atagtctttg gttgtgcatt aatagcttta aaacgtaaca cctttaggga
    33001 gacaacgtat atgatattga gtgcagttgt tattgctgtt atacttaata tgttagttcc
    33061 atttcatttt ggttaggcag attttaagat tattatagat agaatataaa tttcacgaac
    33121 cgccgaagcg atccgccagc ggtggcggtc gtaagacata tcgccacgcc agcttgctgg
    33181 cgaggtggcg gttcactttg gcggtttggc gagcctgcga gctttctaag cccccgttat
    33241 ctaacagggg gggactatta caaaagacaa gagcaaaaac aaatataaaa ctgctgatat
    33301 gcaacgattt ataaggttgt aagaacttat ttcactttac accctattct ttctacccga
    33361 caggtctaaa acagcatatc aatagaaaat aaatataaga tacaaggtgt atgctcctat
    33421 gagtatatgc ctttttttga ttggaggaat gaagcatgaa tgatatgaaa tttattgaaa
    33481 cattaaagca aaagagaaat gtctgtgatt attcgcaatc acgactggca caggaactac
    33541 aaatcaacag gcagaactta aatgaaattg aaaacggaaa aacaaaagcc agtaaagaaa
    33601 tgaaacatat actcttacat tatcttgatt attgtaattg tacacagcct ttcactttaa
    33661 cgattgacta tctgcgtgtt cgttttccta ccacagacgc attggaaatt ataaaaaatg
    33721 tgctggcaat gaaaagcgaa tattttatac atgaagatta cggaatgtat gcttatgaag
    33781 aacagtatat ctatggggat attagtgtaa atgcttctaa agatagttct atgggtgttt
    33841 tattggaatt aagaggtatg gggtgtcggg acttagaata tgttttacag gtaagaggga
    33901 tagactggta ttgcttttta agatgttgta tagattatca aggtgttttt aaacgcatag
    33961 atttagcagt caatgatatg ggcggattgc tggatataga aattctaagg gaacgttatt
    34021 atgctaacaa ggtatggaaa cgttcaagaa cccatgaagc agtagacagc ggaaaattat
    34081 cgggtacaca tggagatact gcaaaaactt tttatatcgg ttcaaagaat agttctatct
    34141 acttttgctt gtatgaaaaa gaaaaggaac aaaaaagcaa gggtataaaa acagatatta
    34201 agaaccgctt cgaaattcgt ttaaaaaatg gaaaagcagg acaaacggta gagcaacttg
    34261 ttttttcaag aaatcccgaa cagaccatag caagctttat tctcacacag atagattttc
    34321 ccgattatat tttatgggat atatttttag ataatgtaac gacctcactt ccgtttatta
    34381 tgacacctgt tgctgttaat atggataaaa taaaacgctg gctagaaaga caggtcatgc
    34441 cctctttatt gatgataaaa gagattgaaa agaaaacagg agcaaattat ctggaagaaa
    34501 tcgacaggca tacaaggcta acagaaaagc agtaattaaa gattaaacaa atgacaacag
    34561 acatagcaga tatgattgag aaagataccg ctgttccaca agggaatgac ggtatttttt
    34621 aatttcttca aaaaaactgc aacaaaatga ctgcttgtgt acagatatgg tgcgaggaaa
    34681 ggaaatctaa ctttttttga aaacaggtca ttaaatcggt ggctgctgtc cctatatgat
    34741 gtgaaagaag aaacgatttt taatttcaac ttaacatttg acagttttca ttccctatat
    34801 ggagcaaaga aaatctttta ttccatagtt ggcagttacg gtgttgcatg ggtagttagg
    34861 gtgtgaaaag aaaaacagat ttttttcatc accaacttag caaactgata cttgctttcc
    34921 ctatatggtg caggaaaaga gagtttaact attttcattt caacttagca actggacgtt
    34981 tgctgtacag atatgtagtg aaaagagaaa aagttttgaa tattggttac gcttcggctc
    35041 ttttccaacg cggtatatag gaaagacgat tttcttcttt catgttcttt gacaactgaa
    35101 taaagcaatt caatacgttt gacagacagc gatccattga agcagatacg ccatgacctt
    35161 ttccctgcaa aagcgagcga accctatttg tgttcctgta aaagacttgt cctcttttat
    35221 cgccatgacc tgttctgtct gataaatgat actcccgtac agccacagct tgaacgttca
    35281 gagcgtcgca cgcaatgggt acggctacat aagaactatg cagaggtgga attcctgtgg
    35341 gctatgacaa atgccgttga attgcttaga aaaaagatta tggaaagggg gtatgtgtta
    35401 ttgctgatat tgtaaaaaca atgcaagaaa agacaaataa taagcgtagc attggcaaca
    35461 gaggaaaaac aaagattgtt gtaaaagagc atttttccga aaagggaaag acaatggagg
    35521 aacttctgac tgatgttatg ctggaaaaag caaagcagac aatcgcataa aattggtgca
    35581 gttgtaagac atagattgaa tggcaaaaag tacccgtgtt atactttact tgcaagcagg
    35641 tattgtaaac acgggttagt tataaaggag gataactgac aatgaaacaa cagatttaca
    35701 atactgcact ttatcttagg ttaagccgag atgatgaatt acagggcgaa agttccagca
    35761 ttaccacaca aagaagtatg ttgcgtctat atgcaaaaga acatcatttg aatgtgattg
    35821 atgaatatat tgatgacggc tggtcgggaa caaattttga aagaccgagt tttcaaagaa
    35881 tgattgagga tatagaggca ggaaaaatca actgtgttgt aacgaaagac ctttcccgac
    35941 ttggcaggaa ctatattatg acaggacaat atacagaatt gtattttccc agccataatg
    36001 tccgttacat agcgattgac gacggtgtag acagcgagaa aggcgaaagt gagattgcac
    36061 catttaagaa catcatcaat gaatgggtgg caagagatac aagccgtaaa gtgaaatcgg
    36121 catttaagac gaaatttgca gagggtgctc attatggtgc ttatgctcca ttaggatata
    36181 agaaacaccc tgacatcaaa ggaaaactgc tggttgatga tgaaacaaaa tggattgttg
    36241 aaaaaatctt ttccctagcc tatcaaggtt atggtagtgc caaaatcaca aaggtactgc
    36301 gagaggaaaa agttccgaca gcgtcttggc tgaattttac aaggtacggt acttttgcac
    36361 atatctttga gggaaagccc gaaagcaaac gttatgagtg gacgattgct catgtcaagg
    36421 caatcttaaa aagtgaggtt tatataggca acagcgttca caatatgcag tctacggttt
    36481 cattcaagag caagaagaaa gtgcgtaagc ccgaaagtga atggtttcga gtggaaaata
    36541 cccatgaacc gattattgac aaagaagtgt tctatcgtgt gcaggaacag ataaaatcaa
    36601 gacgtagaca gacaaaggaa aaagcaacac cgatatttgc agggcttgtc aagtgtgcgg
    36661 attgtggctg gtctatgcgg tttgcaacga atacggcaaa taaaacgcct tacagttatt
    36721 atgcttgcag ttactacggg cagtttggca aaggtacttg ttctatgcac tatatccgtt
    36781 atgatgtgtt gtatcaagcc gtattgaaac gattgcagta ttgggctaag gcagtacagc
    36841 aggacgaaga aaaggtgctg aacaagatac agaaagcagg caatgcagag cgaatacggg
    36901 aaaagaagaa aaaggcaagt gcctttaaga aagccgagaa ccgacaaaat gaaattgacc
    36961 gtttgtttgc gaaaatgtat gaagatagag cctgtgagaa gataacagag cgaaattttg
    37021 tgatgttgtc tagcaagtac caaaaagaac aaatagaact ggaacagcag ataaccaacc
    37081 taagagaaga attaagtaaa atggaacagg atatgatagg tgctgaaaag tggattgagt
    37141 taatcaagga gtattccgta ccaaaggaac tgacagcacc gttattaaat gcaatgatag
    37201 aaaaaatcct tattcatgaa gcaataacga atgaggataa cgaaagaata caggaaatag
    37261 agatatatta ccgatttatt ggaaaagtag actgataaag agggttcata tctttaacta
    37321 agggaaactg gagaatctat tcctgatata ggtagttgca ttaaacttgc aaaattgtac
    37381 aaggtcaaac tagatgactt agttaatcat tcggaagaaa aaacaggtat tatagtacct
    37441 cctaaaggaa agtttttctt tggagcagta gtagttggtg aacgtggtca gattgtcatt
    37501 ccaaaagaag caagagaagt atttaatata aatgcaggag ataaattatt ggtacttgga
    37561 gatgaggaaa gaggattagg aattgttcat caacgtgatt tgataaattt tataggtcat
    37621 atgggagtag tacgtaaaga agaatgattt taaaaaatta taaaaggagg tatgaaatgt
    37681 gagtaaaata gtatttttta gcattcttgc atatggtcat actaatccta ctattgaagt
    37741 agttcgtgaa ttagtggata gaggaaatga ggtattgtat tattctttta atgaatttaa
    37801 agataaaatt gaaggtgctg gagcaaaatt tatttgttgt gataaatacc ttccagaatt
    37861 actccctgga gatgagaaaa aaattggtaa ggattttcct ggattaattg agatgatagt
    37921 agatactact atttctttag atgaaaaagt ttgtagggaa cttaaagatt ttaatccaga
    37981 ttgcattgta tcagattcat taagtttttg gggaaagtta tttgcacaaa aacttaatat
    38041 tccatatgta tgctctacaa caacttttgc atttaataaa catacagcaa agttgatgaa
    38101 acaaaacttt atagaaatta tacgtatgat ttttggtgta agacgcatta ataaaaaaat
    38161 aaagctttta caaagaaaag ggtatgaagt aaaaaatttt atttctatag tttctaatga
    38221 taatgatacg gataccattg tatatacatc aaaagaattt caaccaatgg tagaaacctt
    38281 ctctagtaag tattcatttg taggtccatc agtatcaaag ttgattatta aacctaaaga
    38341 gagaaaaaga aagttaatat atatttcgct tggtacaatt aataataaaa atgttagctt
    38401 ttataaaaac tgtataaatg catttaaaga ttctgatgtt gatgtgatta tgtctgttgg
    38461 tagaagtact gatattaaaa gtttaggaaa tataccaaat aattttgagg ttaaaaatag
    38521 tgttgaacag attgctatac ttcaaaaaac tgatgtgttt attactcatt ctgggatgaa
    38581 tagtgttaat gaaagtttat attatggagt acccatggta ttatttcctc aacattcgga
    38641 gcagagaatg gtagctaaga gagttgttca tttgggagca ggaattatgt taaaagagga
    38701 taagtccgaa agtattaaaa aagcagtatt tcaagttata aatgacaatg aatataaaga
    38761 aaatgcaact aaactatcaa aaagctttta taatgcagga ggctcaaaaa aagcagcaga
    38821 tgtaatatta cagattatat gtaatagtag acaagttgac atataataga tttgttaaaa
    38881 atatatatac atacatgcaa tttaatcaaa gtactgacag tggggtcttt taacctacca
    38941 gttggggcat atatattgat gctaaatatg agggatgtta tattttaata aaaaagataa
    39001 catccttttt ttagtgcgtt atatcaagtt ttaaataata aatgaaggag gattaatatg
    39061 aaaaaatata aagttatttt tatagtatta attataggta ttataagtgt aacagggttt
    39121 gttgtatata aaaactgcat taaaccatgg aaagatgctg aaacatatat tgataagtat
    39181 atggtaaaac aagatatttc aaaagataat ataaaaagta ttacaaaaga aaaagccaag
    39241 aaggcaagtt acgaaggaat actatacaaa gtgtattaca aagatgaccc tcgatatgag
    39301 tatcaatatt tttatagtga taattatcat gctctatatg taaataaagt aatgttacaa
    39361 atttatgatg tggaaaataa taataaactt ttaagaagta atgaggaatt aaaaagtgta
    39421 aagtatccac caatatatct taaatataag ccagataaat gat
//
LOCUS       DAEPLF010000016        38741 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEPLF010000016 DAEPLF010000000
VERSION     DAEPLF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38741
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.016"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..267
                     /locus_tag="KR314_000721"
     CDS             <1..267
                     /locus_tag="KR314_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0327121.1"
                     /translation="FNMKEEAKKALYSLKEILEKYPIHESLDVSQMRFFDKVESKNLY
                     TFNIYTNLLIVLNDDSYNELREEPIFKDVIEKILDMEKMLKNKE"
     gene            complement(687..1208)
                     /locus_tag="KR314_000722"
     CDS             complement(687..1208)
                     /locus_tag="KR314_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0327122.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSYLQTEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            1564..2361
                     /locus_tag="KR314_000723"
     CDS             1564..2361
                     /locus_tag="KR314_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBH0327123.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            2471..3061
                     /locus_tag="KR314_000724"
     CDS             2471..3061
                     /locus_tag="KR314_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBH0327124.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNEETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFADFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            3127..4824
                     /locus_tag="KR314_000725"
     CDS             3127..4824
                     /locus_tag="KR314_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBH0327125.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGISLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     NVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            5038..5877
                     /locus_tag="KR314_000726"
     CDS             5038..5877
                     /locus_tag="KR314_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBH0327126.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            6394..8250
                     /locus_tag="KR314_000727"
     CDS             6394..8250
                     /locus_tag="KR314_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBH0327127.1"
                     /translation="MDIHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYRGGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIIDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALINKIFESDKGVVENKKVVTFELNVSINDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSENNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    8333..8573
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    8639..8874
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8959..10176
                     /locus_tag="KR314_000728"
     CDS             8959..10176
                     /locus_tag="KR314_000728"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBH0327128.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRSIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            10290..10784
                     /gene="cdeM"
                     /locus_tag="KR314_000729"
     CDS             10290..10784
                     /gene="cdeM"
                     /locus_tag="KR314_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBH0327129.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGGNRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            11049..12314
                     /gene="hisD"
                     /locus_tag="KR314_000730"
     CDS             11049..12314
                     /gene="hisD"
                     /locus_tag="KR314_000730"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBH0327130.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(12517..13269)
                     /locus_tag="KR314_000731"
     CDS             complement(12517..13269)
                     /locus_tag="KR314_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0327131.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANKKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(13387..13656)
                     /locus_tag="KR314_000732"
     CDS             complement(13387..13656)
                     /locus_tag="KR314_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327132.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            14131..15225
                     /locus_tag="KR314_000733"
     CDS             14131..15225
                     /locus_tag="KR314_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBH0327133.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LRERSINGVISMCLKETDYQYSLSNKKGGY"
     gene            15227..16177
                     /locus_tag="KR314_000734"
     CDS             15227..16177
                     /locus_tag="KR314_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBH0327134.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGLKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKIDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            16170..17354
                     /locus_tag="KR314_000735"
     CDS             16170..17354
                     /locus_tag="KR314_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327135.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKKSLMREMNNVSKGVIDNEDTPSGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKSLLEISSYFKSKGLKIGIDTFSPFISYFTGQDLKKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            17367..18863
                     /locus_tag="KR314_000736"
     CDS             17367..18863
                     /locus_tag="KR314_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBH0327136.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNSHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHTVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSEGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            18886..19836
                     /locus_tag="KR314_000737"
     CDS             18886..19836
                     /locus_tag="KR314_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0327137.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLVSFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            19857..20819
                     /locus_tag="KR314_000738"
     CDS             19857..20819
                     /locus_tag="KR314_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBH0327138.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            21191..21823
                     /locus_tag="KR314_000739"
     CDS             21191..21823
                     /locus_tag="KR314_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0327139.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            22234..22326
                     /gene="kdpF"
                     /locus_tag="KR314_000740"
     CDS             22234..22326
                     /gene="kdpF"
                     /locus_tag="KR314_000740"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBH0327140.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            22367..24049
                     /gene="kdpA"
                     /locus_tag="KR314_000741"
     CDS             22367..24049
                     /gene="kdpA"
                     /locus_tag="KR314_000741"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBH0327141.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            24074..26131
                     /gene="kdpB"
                     /locus_tag="KR314_000742"
     CDS             24074..26131
                     /gene="kdpB"
                     /locus_tag="KR314_000742"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBH0327142.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNVGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMIL
                     NLG"
     gene            26150..26776
                     /locus_tag="KR314_000743"
     CDS             26150..26776
                     /locus_tag="KR314_000743"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBH0327143.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNASYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNIEVAKILGLI"
     gene            27013..27921
                     /gene="cysK"
                     /locus_tag="KR314_000744"
     CDS             27013..27921
                     /gene="cysK"
                     /locus_tag="KR314_000744"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBH0327144.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            27948..28538
                     /gene="cysE"
                     /locus_tag="KR314_000745"
     CDS             27948..28538
                     /gene="cysE"
                     /locus_tag="KR314_000745"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBH0327145.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            28698..28886
                     /locus_tag="KR314_000746"
     CDS             28698..28886
                     /locus_tag="KR314_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBH0327146.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(28940..29809)
                     /locus_tag="KR314_000747"
     CDS             complement(28940..29809)
                     /locus_tag="KR314_000747"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBH0327147.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDMNKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(30006..31250)
                     /locus_tag="KR314_000748"
     CDS             complement(30006..31250)
                     /locus_tag="KR314_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327148.1"
                     /translation="MENNLNIAKKNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYYMENNTNITIYENNTLQYKFDEESEDIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKSRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            31541..31852
                     /locus_tag="KR314_000749"
     CDS             31541..31852
                     /locus_tag="KR314_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBH0327149.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      31945..32037
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            32137..32931
                     /gene="thiD"
                     /locus_tag="KR314_000750"
     CDS             32137..32931
                     /gene="thiD"
                     /locus_tag="KR314_000750"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBH0327150.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            32955..33743
                     /gene="thiM"
                     /locus_tag="KR314_000751"
     CDS             32955..33743
                     /gene="thiM"
                     /locus_tag="KR314_000751"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBH0327151.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGIYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            33766..34398
                     /gene="thiE"
                     /locus_tag="KR314_000752"
     CDS             33766..34398
                     /gene="thiE"
                     /locus_tag="KR314_000752"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBH0327152.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITERYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEATCAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            34537..34839
                     /locus_tag="KR314_000753"
     CDS             34537..34839
                     /locus_tag="KR314_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0327153.1"
                     /translation="MIKILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRVPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(34905..35654)
                     /locus_tag="KR314_000754"
     CDS             complement(34905..35654)
                     /locus_tag="KR314_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0327154.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(35648..36610)
                     /locus_tag="KR314_000755"
     CDS             complement(35648..36610)
                     /locus_tag="KR314_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327155.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVKMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(36614..37072)
                     /locus_tag="KR314_000756"
     CDS             complement(36614..37072)
                     /locus_tag="KR314_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBH0327156.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            37428..37784
                     /locus_tag="KR314_000757"
     CDS             37428..37784
                     /locus_tag="KR314_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0327157.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            37856..38572
                     /locus_tag="KR314_000758"
     CDS             37856..38572
                     /locus_tag="KR314_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327158.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            38565..>38741
                     /locus_tag="KR314_000759"
     CDS             38565..>38741
                     /locus_tag="KR314_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0327159.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIF"
BASE COUNT        15031 a         4165 c         6755 g        12790 t
ORIGIN      
        1 tttaacatga aagaagaagc aaaaaaagcc ttatatagtc taaaagaaat attagaaaaa
       61 tatcctatac atgaaagttt ggatgtaagt caaatgagat tttttgataa ggtagagtct
      121 aaaaatttat atacttttaa tatatataca aacttgttaa ttgtacttaa tgatgacagt
      181 tataatgagc ttagagaaga accaattttt aaggatgtaa ttgaaaagat tttagatatg
      241 gaaaaaatgt tgaaaaataa agaataagta aaaatttaat ataggactta aagattaatc
      301 aaattaaaag aaagtgtttc aaaataaaaa tttgaatcac cttttttgtt ggtcttaaac
      361 cgttatttag aaaaatagag gtttcaaaat agaaatctga attatctgta ttattacaca
      421 aaaatagaaa cctaaattta taaaaataga ataaagtttg ttatataaaa tgtaattttg
      481 tctataaaat ctcacatgtt ttcttaactt taagcgtgaa aaagaggatg tatcatgaat
      541 tttttagttc atgatgcatc cttatttgta tgaaatccaa tatatcttta ttatttaaac
      601 aatggaaaag agtcttattt ctattttgag acatctcttt tatttaatta tgaagtttta
      661 tacagttcaa taaataggaa ttatatttag aaactcatga cattatttct tagatagtga
      721 ttaaatttat catattccat tttagataac ttttctgggt aagacattac aatcaggtta
      781 tgcccattga atttttcatg gtatggtgga tgaatgtgtt cataattatt tgtcttatat
      841 tttactcttt catagaacgc cttgcgataa attgaaatct catcaattgg aaggtcaatt
      901 tctaaaataa tagtttttcc tttcttattt aaaagctcta acgctctttg tccatatcta
      961 ttattgcgca tttctggaaa aatgcagaaa tgttctacat agataaagtt atctgtttcc
     1021 caacataaaa ttatgccgac aaactggttt ttatcataaa ttaaattgaa ttgatattct
     1081 ttatttttca aaatttctgt ttgcaaataa gattttcttt gttcatgaaa tgggaaacta
     1141 attttatata attccatagc cttgtaatat aatttatttt cactggttct taaccgttca
     1201 aatttcactt tactcctcct aatcacgaaa attccaattt atttatgagt tcattttacc
     1261 atagtcagta tttgcataca atgtgtttta agttgagtgt catttctttc ttttatttat
     1321 gcttatatct catagactaa gagataattg cttaagacat aactaaaaac tattatatta
     1381 agctttgata atctaaaaac agtatattta tggacttttc taggtctaaa agtaatctta
     1441 ataattttag aagttacaaa aaagttacat aaatttggaa agctataata tacatatata
     1501 atataaggtt aaaactgtaa tgaataatat taatttaaaa tttgaacatg gaaggagatt
     1561 attatggaga aaagagaaaa gaggataata atatcatctt tattaagtgt atcaatttta
     1621 atgggtttgg taagtatata ttctatatta aataaagaag acataatttt aactgttaag
     1681 ggtcaagagc aaaaagtatc ttcctttaag aaaacagttg aggaactttt ggatgaacag
     1741 ggtgtaaagt ataattctga agataagatt aatccaagtt tagatacaga actaaaagat
     1801 gatatgaaaa taaaagttgt taaggtaact aaaagtaaaa aagaagagat tgaaaaaatt
     1861 ccatttgata caaagcatgt aaatgatagt aatttgttaa aaggaaaatc taaagtttat
     1921 caagaaggtc aagaaggaga aaaaaaacta gtctataatt taacttacca tgatggaaaa
     1981 ttagtcaaaa aagtcttatc aaaagaagta atatctaagg agccaactac aaaaattata
     2041 aaatatggaa ctaaagaaaa agtactaata gcatcaagag gagcaaatat aagaggaggc
     2101 aaacatatga aagtggttgc cactgcatat gcaggagata caataacatc tactggtaca
     2161 actccaagat ggggtgttat tgcagttgac ccacgtgtaa taccatatgg aacaaaagta
     2221 tatataccta aactgggtat gacttttgta gcagaagatt gtggaggtgc aattaaaggt
     2281 aatagaatag acatttttat gaacagtgaa ggaaaagctt caaattgggg aaggaaaagt
     2341 atagatatat atctacatta aattagtgaa tagaataact ttacttgaag gacaaaaaag
     2401 tataaaatat gaattaacta aactgttaga ttattcttaa aacataaaaa tatatctaag
     2461 gggggataag atgaaaatag tctgcttagg agacagttta acgtatggat ttggagtatc
     2521 acgaagtaat tcatggacaa acattgtaaa tgaagagact cgattagaaa tagtaaataa
     2581 gggtataaat ggtgatacaa caagtggtat gttagctagg tttaatgaag atgttgtaaa
     2641 aaactctcct gatatagttt ttataatggg tggcactaat gattttattg ctggtgcggg
     2701 aaatgaaatt attaattcta atataatggc tatggtgcat cagtcatatt caaaaaatat
     2761 tatacctata ataggtatac cattaaaacc agatattcca aatgttaggg aagattggag
     2821 ttgttttact gattttaata tagtatctca aaacttagaa tcatatagtt attggataaa
     2881 aaaattttgt atgacattta atactaattt cgcagatttt tattcagaat ataataaaaa
     2941 tatggaacta gaaggatata aaaaattata ttttgatggg ttacatccaa ccaaagaggg
     3001 tcatagaata atggcagaca tttttattaa ttcaattaat aagtatatag aagagttata
     3061 gattacatta aaatgcttgt ttatgcgtca gttgcaataa atacatacag tagagagggg
     3121 tttattatgg attatactaa aacttatgaa gagtggataa agggttcata ttttgatgaa
     3181 gatacaaagc tagagttgga gaatataaaa aataatgaaa aagaaataga ggatagattc
     3241 tataaggatt tagaatttgg gactgcgggt cttagaggaa taattgaagc aggaacgaat
     3301 agaataaata agtacacagt tagaagagct acttttggat tggcaaacta tatattagaa
     3361 aacacaacaa aagaagaaac aagtagagga gtagtaatag ctcatgataa tagacacaag
     3421 tctagacaat tctgtataga atctgcaaat actttggcag catgtggtat aaaagcatat
     3481 atatttgata gtttgagaac tactccagaa ttatcttttg ctgttagaag cttaaatgca
     3541 atcgctggta tagtcataac agcaagccat aatcctccag aatataatgg atataaggta
     3601 tactgggaag atggagcaca agttatgcca gagattgcaa atgcaataac agagaaagta
     3661 aatagtatac atgattatag tacaatacca accttaacgg aagctaataa aaatttggta
     3721 gttttgttgg acgaatcaca agatactaaa tttatagaag cagtaaaaag ccaaattata
     3781 agaaaagatt tagttaaaaa tgtaggtaaa agctttaaga tagtttatac acctctttgt
     3841 ggaacaggga atgttccaat aagaagagca ttgaaagaag ttggttttga aaatataatt
     3901 gtagttccag aagaagaaaa tccagaccca aattttgcag ggcttgatta tccaaatcca
     3961 gaagagaaaa aagctttaaa tagaggtata ttactcgcaa aagaaaaagg agcagacctt
     4021 gtaatagcaa ctgacccaga ttgtgataga gtaggtgtag cagtaaaaac tactacaggt
     4081 gagtatgctt tacttacagg aaatcaaatt ggtggtatgc tgacacatta tataatagaa
     4141 ggattaaaag aaaataacaa attaaaagaa aatcctacaa taataaaaac catagttaca
     4201 tcagaatttg gagcagatat agctaaagca aataatgttg atgtattaaa tgttcttaca
     4261 ggttttaaat ttattggtga aaaaataaaa ttatttgaac aaaataaaaa tagaagttat
     4321 gtatttggat atgaagaaag ttatggttac ttagttggga ctcatgctag agataaagat
     4381 ggtgtagttt cttcattgtt aatatctgaa atggcagcat tttactattc aaaaggtata
     4441 agtttatatg atggtttaat agaactttat aaaaagtatg gatttttcaa agaacagact
     4501 atatctttga ctttaaaagg tatagaaggt gtagaaaaga taaaagaaat aatatcttat
     4561 tttagagaaa atcaaattga ttatataaat agtattaaag tagttgataa aaaagattat
     4621 aaaaatggta tagataatct tcctaaatct aatgttttaa aatacttttt ggaagatgaa
     4681 tcttgggtag ctataagacc atctggaaca gaaccaaaac ttaaatttta catagctgta
     4741 aaaggagcta gtgatattga agcagataaa aagattcaag gtttgaaaaa gtatatagat
     4801 gacatggtcg aaaaattaaa ataaaatatt attattttat aagataattt tgtaataata
     4861 tttatttagc tgaaaattat aatttacttt cattgagtag aatatggaaa tgttagtaga
     4921 attgttattt tcagctttta tttgtgttat ttttacttaa ataattatat tatagaatga
     4981 atctgagtat aattaatatt gtataatgaa attaatttga tagaaaaggg gatatttatg
     5041 tttggtatag ataagaaatt gattgaatat ataattgcaa aatccaatta cagtgaatta
     5101 agttcaaagt cagcagaagt atctttcttc ttaatgttgt ccatatttcc ttttttaata
     5161 tttacaataa gttctatagc ttatataccg attcttcatt taaataagta tatagctttg
     5221 tttagaaata tgatgccaga aggtgctttt gctgtgttat cttcaatcat agtttctgca
     5281 atagacaata gaaacctgaa atttttagca gtcagttttg tacttactat gtggacattt
     5341 tcaagagcag taaaagctct tattaaagga atgaatagag cctataaagt taaagaaact
     5401 aggtcttttt ttaaaatttt atctatatca tttttattta caataatgct tttggtcctt
     5461 atttttttat ctatgatatt tttagtttac ggggaaaaaa taggttattt tatttttaat
     5521 cttgtaggat tggatgagat atttataaaa atatgggata ttctaagata cactgtaggt
     5581 ataataacaa taatagtgat atttacactt ttatataaat acactccaaa taaaaagtta
     5641 actattaaag aatctgcgcc aggtgctatt tttgctactt ttgcttggtt tttagtatca
     5701 tttttatatt cttattatac aaattactat gctaactatg aagtaatcta tggaagtata
     5761 gctgaaataa ttgtacttat gacctggatg tattttagta gttggtctat cgttattggc
     5821 tatgaggtaa attcaagatt gtattttaga aagataagac atgaaatgtt aaagtaaagt
     5881 atttaaaata aaagagagta tttagtatta taaatgtaat tgaattaata aagaaatatt
     5941 gacatttaag ttaaaaagtc tagaattttt ctgggctttt tttattttta gtataacttg
     6001 acaaatttta tttaatggga tagttttaat ttataatcat ctttgaaaag atgactattg
     6061 ataagtaagg tataaaattg gtaagtatca aaaaatatac taaataaaaa ttatagtcct
     6121 aatgagttaa tttctgtatt ttcaaataat gcagtattta gtcatgtgtt tttatatgcg
     6181 cctgtatttc caattaccaa tatattagaa taagctactg taattagatt tagaaaaaag
     6241 cgacataaat aattataaac aaataataaa ttacataaaa ttaaatttat tcacaagtat
     6301 atcttataaa atatgttata attaatagaa aattgtcata taattaatta agtagtatta
     6361 ttataaataa cattgaaagg tagtggttag aatatggata tccataataa atatgtaaat
     6421 tttataaaaa atatacctgt acctttttta tattgtagaa ttgtaaagag acaagaagat
     6481 atagaatatc gagtagaata tataagtaag ggtatgggca aagtattaca attagaagaa
     6541 ggtatttgtg ataaaaatat actagatgta ttgcctgtat ttaagtctaa aaaatatttc
     6601 aaagagttgt tctcaaatga agtggattgt ataaaaaggt acattccgac acttaaaaac
     6661 tggataaata taaaaaaaca gataataggg gattcataca taattttgta ttttggtaaa
     6721 attgtatttg attatagaca gataatcgat tcctttgata aaaaggaaaa agtagcttat
     6781 attaaagacg aagaaggcat atatatagat tgcagtgaaa atttaatacc aatattaaat
     6841 aacaacatta aaacaacgaa agatatcttt ggtaaaaatg atatagaagt atggggagag
     6901 aatacaggaa aattatttag agatgattat agaggaggag tatctagtaa aaaaagattt
     6961 ttacaaaatc tttttgaata tgaagaaaca ttttttatgg ttgaaaaata ttttttgtat
     7021 gatgaagacg aacttttagg tactataggg ataatagata atataatata tagtggttac
     7081 agtaatagaa attataactc aaaagattta atgaagatga tagaacactc aattccagag
     7141 aatatgtttt ataaagatgt gtatggaaat tatattggat ttaattcagg ttttttgaat
     7201 ttagcttgta tgaataaaga agaattatta gggaagaata gttataaaat atcagaagaa
     7261 gaagctttaa taaataagat atttgaaagt gataagggtg tagttgaaaa taaaaaagta
     7321 gttacatttg agttaaatgt aagtataaat gatgaaaata agtgtattga gattacaaaa
     7381 aggccgtttt ttgatagcta tggaagtgtt attggaataa ttggaacagc aagggatata
     7441 agtagaagaa aaagattaga agaggaaatg gataaaacta ggatggaatt ttttgcaaat
     7501 ttatcccatg aacttagaac gcctataaat ttaatatcat cttcattaca agtgatagaa
     7561 aaaaaagaag cagatttaat agaatcaaat gatactttaa aaagaaattt aggtataata
     7621 aaacaaaatg gaaatagaat actgaggtta gtaaataatg ttatagactt tacaaaaatg
     7681 caatcaggat atttagattt taaaccagaa gaatctgata taattgcatt tatagaggag
     7741 atatgtatgt ctgtagctga ttttgcaagc caaaataata tacaacttac atttgataca
     7801 gaaatagaag aattttcaat gttatttgat tcagaaaaat tggaaagaat aatattaaat
     7861 ttactttcta atggaattaa gtacaataag aaagatggaa aaataaatat atttttatat
     7921 gtgaaagata atgtatttaa tatgaaaata tctgatagtg gaataggtat accaaaagaa
     7981 aaaatagata aaatatttaa tagatttgaa cagattgaca atgaactttc ctacagagtt
     8041 aaaggaagtg gtattggact ttcattagtg aaatctttgg ttgaattgca tgagggaagt
     8101 atatctttaa aaagtcaact tggtattgga agtgaattta tagtttcatt acctgttaga
     8161 agtgaaaata atattgaaaa atataatcat aaaagagaaa tcagcaatga actaagtaaa
     8221 aaattagaaa tagagttttc ggatttataa gtttaaatat aaaaaataaa attttaaaaa
     8281 agtattgaca attgatttag aagggtttaa aataaattac aacataataa ataaaaaggc
     8341 gatgaatagg aagagtaaat ctaattcgat taccagagag aagagtccat tggctggaag
     8401 actttttaaa ttagagggat tgaaaactac ctaggagcta ctttctgaag tcatctgcat
     8461 tagtagattt tagtaagaat agtcgttagc tgcgttaaag tgtaaagtga gttttcatga
     8521 tatatgaaaa ctaaattagg tggaaccgcg ggaataatac tctcgtcctt atgttataag
     8581 gatgagagtt ttttgttgtt taaaaatata aacagtaatc ataataatta tagaaaatat
     8641 aaaagcgatg aataggaaga gtaaatttaa ttcgactatc agagaggaaa gttcacaggc
     8701 tgaaagactt tctaagtttg agaaattgaa aactacctag gagctgtggt ctgaagttac
     8761 tttaaaaagt tttagtaaga ctaaccgtta actgcgttaa agtgtaaagt ggattttatc
     8821 aataaatgat aaaatcaaat tgggtggaac cgcgagtata gactcgtccc tttatatagg
     8881 gatgagtcta tttttattgc aataaaagat tgaaaataaa aaaaaatcgg aaaattataa
     8941 atttaggagg aaaataaaat ggaaaataaa gtgaaaattg gagtactagg atatggagtt
     9001 gtaggaagtg gacttataga tataatagat aataataaag aaaaaagaag tatagaaata
     9061 gttggaattt tagttaataa tttggaaaaa cataaggata aaaaatattc taatataata
     9121 actaataata tagatgatat atttaataaa gatatagata tacttgttga agtcatgggt
     9181 ggtttagaac catcattatc ttatataaaa aaagcattaa acaataaaat tcatgttgtt
     9241 acagcaaata aagatttgtt agctgaatgt ggagatgagc ttgcaaaatt agcaagtgaa
     9301 aataaagtaa gtattaaatt tgaagcttca gtcgcaggag gtattccagt attaaaacca
     9361 ataatagaat cactagaagg aaataatata gatagtataa atgcaatttt aaatggaact
     9421 actaatttta tattatctaa aatgtatgat gagaatctat cttatgatat ggcattaaga
     9481 caagctcaag aattaggttt tgctgaagca aatccagagt cagatgtatt agggtatgat
     9541 gctgctagaa aactatcaat attatcaact ctagcttatg acaatagagt atattggaag
     9601 gatgtgtatt tagaaggaat tacagatata gatgaaaaag atattgaata tgcaaaaaaa
     9661 ttaaattgta aaataaaatt gattggtcaa agtaagtatg aaaatgataa agtaagtgct
     9721 tttgttagac ctgttttagt agaaaaagat aatatccttg ctagaataga caatgagttc
     9781 aatgctgtca tagtgaatgg agattcagta ggagaagttt cttttgtagg taaaggagct
     9841 ggaagtttag caacaggaag tgcggtttat tcagatgtta tagatataat agataataga
     9901 gtttcaagta tagattcttt tactaaagat aaaatacaag ttaataaaat agttagagaa
     9961 aagtgtggag cacttcttag atttaaaaaa tgtaataaag atgaaattct aaatatagtt
    10021 gagaattgtt tagtaaaatt cgatatttta aatgatgatg atgaacttgc tattatggtt
    10081 tatgctgatt ctgaatatga aataaacaac agtttatgtc taataaaaga taaaggatat
    10141 tgcgaaaaaa tgaataaaat gttgaaaata agttaaaaaa gaaaaaataa gtttatatgt
    10201 aacatatttt gacgatttta atactatata gtatgagatg aaacaaaaca tctcataaaa
    10261 aattaaaatt caagggagag atatgttata tggaaaataa aaaatatgca aatggtggtt
    10321 attcagaaga ctggtatgaa agaggagaat ctacagctaa atggttccag aatgatagag
    10381 aagaatatga aagagcagca tacgatgaag atagagaaag aagaggctca aactgtggat
    10441 gttcagattc aggaggaaat agacctagaa actgtgaaag atttagaaga gaagctgaga
    10501 taagagaaag agaagcaaga gaagcattct gtgaatcttc agagagaaag aaagaagcac
    10561 tagcatatga atgtgaagct agaaaattat gggaagaagc agaaaaatac tgggatgaat
    10621 attcaaaata caactataaa ggaatcgaat atttagcaga agctgctaga ttatttgatg
    10681 aaggtatgga atgtgaagct agaagaaatg gaaataatgg aggaaacaat aataattgtt
    10741 gccataaatg ccataaatgt aattgtaact gctgtagaaa ataaaaaaat tcattgacaa
    10801 atcaatagtt aaaatatatc atattatgta taataaagag aaaattcaga aaaaaataat
    10861 gattagaata aatttaagta tatttattag acaaatagtg ctcagttagt gaggtgagta
    10921 caatatgatt aaactcccat cctctagctt ttgatagagg gtgggagttt ttatttattg
    10981 atatatctta aatgaggata atattgtaaa ataaatgagt atgagattgt aaaattagga
    11041 ggtaaaaaat gaaaagtatt aaaacatcaa gtatgaaaga aaaggtagat gaaaagatat
    11101 taagagagca agtaagttct ataatagaag atataagaaa taataaggat gttgcactta
    11161 aaaaatataa tgagaagttt gatagaaata caagagatga atttagaatt actaaggaag
    11221 aaataaagga agcttataag catgtagatg acgaatttat aaataactta aagattgctg
    11281 ccaaaaatat aagagaattt gctaaagctc aaaaactaag ttttgaaaac ccttttgaaa
    11341 aagaaattta tccaggtgta atcttaggtc aaactaatat tccaatagag tcatgtcttg
    11401 catatgttcc aggaggtcag tatccattgt tttcaacagc tcttatgctt ataatacctg
    11461 caaaagtagc aggagtaaaa agaatagtgg catgttcacc aactatgaaa aatactgaaa
    11521 agataaatcc taaaacattg gtagctatgg atatagcagg tgctgatgaa atatatgcaa
    11581 ctggaggagt tcaagctata gcagcattta catatggaac agaaaagatt aatccagttg
    11641 acataattgt aggaccagga aataagtttg taactgaagc taaaagacaa tgttatggac
    11701 aagtaggaat tgattttgtc gctgggccaa gtgaagttct tataatagcg gatgaaactt
    11761 caaatccagt ttatattgca gctgatttat tagcacaatg tgagcatgat ttaaatgcaa
    11821 gaggaatatt attgacaaat agcttggaag tagcacaaaa agttgaaaaa aatatagaaa
    11881 gtatgctaaa agatttgcca acaaaggaca tagcatatag ttcatgggaa aataatggag
    11941 agataatatt ggttgatgat atggaagaag ccattaaaat aagtaatttt tatgcaccag
    12001 aacatttaga agttgcagta aatgagtata atgatatttg tgatagatta actaactatg
    12061 gttctttatt tattggaaat ctttctgctg aggtgtttgg tgactatgtt tcaggtacca
    12121 atcatacatt accaacactt aaagcatcaa gatatactgg tggtgtttgg gttggaactt
    12181 ttatcaaaac ttgtacaaag cagatattta atgaaaaagc tatacaatca ttagcacctg
    12241 tagctgaaaa gctggcaaaa gaagagggat tatatgcaca tgcaaaggct gctgaggtta
    12301 ggtttaaaaa gtagtaaaaa attttatttt ggcagtcgag atttagtatt agactgagat
    12361 aaagaaaacg ctgagaacct gaataaatac tatattttgt gggcctcggc gtttctttta
    12421 tatctgtata taataggata atactacctg tgtgtttaaa tgctaactta atatttcttt
    12481 tctaaataat gtttatgaaa tagttaatat ttttagttaa tacttgaaaa aaacttttgc
    12541 taactgtgca aattcatctg catttgtcaa tatagcaggg tgtcctccct gactaaaaat
    12601 gtgaatactt gcttttgaaa taaaagttgc catttgcttg tactcttctt caagattatg
    12661 acgacacatt tcgtcttcct tacttcctgt aaataaaatt ggcatttcta atttacacaa
    12721 ttctttatga aataaagggc gtttcttgtt tgcacattgc aatagagctt ttgtatcaag
    12781 attaacaaca ttctcccaat cttttccatg acaccattca tagaattttc gtgcttgaat
    12841 atcttgcttt gatttttccc ttccagtgat tagattatct atgaagttat cattaagagt
    12901 acgtccatca aagctatctg ctataattgc ttcaactaaa tcgggacgtt ctaatccagt
    12961 attaacagct gcccatgctc ctccacttgt tccaattaag ccaactttac cacaattcaa
    13021 atgttcaatc agtgctattg tctgtaaggc ttcatcatgc catatgtcag gagaaaattg
    13081 agaaactcta tcagactgtc cattacctaa aaaatcaatt aagatacatc taaaattttc
    13141 tacatataat ggcacaagtg ctttaaacat attagaagaa ccagtattac catgcaggaa
    13201 aattatcggt ttaccttgtc catattcttt ataaaaacaa gaattacctt gatactgaaa
    13261 atatgacata tattttcctc ctttttattt gttaagttaa ttataacaat ataggtatga
    13321 atgaaaaacc tcatgttctt tcttagaata aaaatgtgcc acgcataaaa cgtgacacat
    13381 ttctaattat aaattttgct gtttaagtat tctttgtatg cttttctctg tcaaaaagta
    13441 tttctctgat aactgcttga tactcattcc attttggaaa tttatacaga tttgagaatt
    13501 acgagaagct aagacttgtt tggtgtttgt gttttcaccc caatgctttt tgtgctcttg
    13561 ctttttggga atataaatca ctttaccatc tatatattct tgcacttgct caatcagttc
    13621 tttaggcagt aagtcaatcg aattttgata gctcattttg cacctcctaa ataattactc
    13681 tttaggatta gcaaaggcta ctgctactca caattaaaaa taaaattttt tattatgtac
    13741 aatagccttt gctatgcaca agttccaatt cccataaact aaatactcct ttctggtttg
    13801 aaaaattcat ctgaacattg atttcgattt taagatagag ttcaatgaca aatttacatt
    13861 tttattatat atattcagat agataattgc aactgtcttt gtatgaggat attttattat
    13921 agtcaatact ataatatata tttatatcat caaactaata tgaacagttg aaaggaatat
    13981 gaacaataaa acttagtcag gaagaataat tatgaagcag acaattgaga aatttgattt
    14041 taaagtttta ttgaaaattt attgacaaat ttaacttgta aatattatac taaacttaat
    14101 aaataaagtt tataaagtaa ggtgttgtct atgaaaaata tagttggaaa accgcaaacg
    14161 ataaaaaaag taaatgaaga tttaataaaa aacattataa aaaatgaagg tcctataaca
    14221 aaaccagaaa tttcaagcat tactgaattg agtttagcta cagttaataa gattgtggag
    14281 caattagctt tgaaaaatga agttaaagta agtggactaa gtgaatctac tggaggaaga
    14341 agagctcaat tatttgaaat aaatgcaaac ttagaacaca taatagcttt atattattat
    14401 agaaattgtt gtataggggt agttgccaat ttattaggtg aaataattta tcaagaagaa
    14461 tttaatataa gaatggatac atatgaaaac gttaaccaag atactttttt tgttatagat
    14521 actctaataa aaaaggaaaa aggaaacaat ataagggcta ttggaattgg tgtcccagga
    14581 gttgtaaaaa aaggtgtagt taaaaatata ccaactataa aaagttggga aggtataaat
    14641 ttagaaaatt taataaagag taagtacaat actaaagtgt ttatagaaaa tgatacaaat
    14701 attactacta ttgggattta tcaaaaacag tataaagaca aatataaaaa tatggctctt
    14761 atgtatttag aagatggtat tggttcaggt atagttataa atggtgaatt gtatttaggt
    14821 tctacaaatt ttgctggaga attaagttat ctaaatatag aatttggtaa gtataactct
    14881 aacaacttaa atcttgaaga ttatattatg agtttaagaa aaagatattt agaaagtaaa
    14941 gataataaat ttaagaaaga aattttatct ataatatcaa actttataag gactcttgta
    15001 tgtgtcctaa atccacaagc tatgattata cagtgtagta tattaaccaa gagtgacatg
    15061 gagtttatag aaaaagatgt aagtaataat gttggagaag aaaattttcc taaattaata
    15121 aaagtagagt cattgagaga aagaagtata aatggagtaa ttagtatgtg tttaaaagaa
    15181 actgactatc aatattcctt atctaataaa aaaggaggat attaatgtga aatatttggt
    15241 ttcaatagat ggagggggaa caaaaactaa attttgtgta agtgacttag atggaaacat
    15301 acttaaagaa cataccactg gttcaactaa ttataagtca gttggaatta aaaaaacata
    15361 tgaaaatatt aataatggac ttaaaaaaat attaaaagat ttatatatcg attatgacga
    15421 tattgagtac actgtatttg gtatatctgg ttgtgattct cctaatgatt ataagattat
    15481 aatggatgaa atattaaaaa ttgggattaa caaagaaaaa atatatctgg caaatgatgc
    15541 agttttagct ttttatgcac aagctgattc ccctggttta gtaattgtgg ctgggacagg
    15601 ttcaattata ctgggaatta aagaagatgg agaaatatat agagttggtg gatgggggta
    15661 taattttagt gatttgggtt ctggatatga tatagggaga aaattattaa agaaagtatt
    15721 gctttattgt gatgaatgtc atgagtatag tgatttattt agttgtgtct tggatttctt
    15781 tggagcaaat agttttgaac aacttgcata tatgattact gatataaaca ataatgttga
    15841 aatagcaaac ctagcttcat tagtgattga ttgtgccgaa caaggtgata aattagctat
    15901 tgaaatatta agagaatcat caactgaact atctaaatta gctcaagtaa tattgagaaa
    15961 aatatctaat ccaatgaaaa tagataaaag cgaaataaat atagttttgt ctggtggtac
    16021 attaaataaa actgtatatg ctcaaatgtt aatgaataat ttaaaaacag aaaatacaga
    16081 taaaaattta aaatttatat ctcaagaaaa tcagccagta tatgggggga taagacttgc
    16141 tatggcatta gctaatagga gagttaagca tggatagaaa aaaggtaata gtacaaattt
    16201 ttactggagg ttttaaggct aaagaaataa agtttaatga tataaaatgt aaactactag
    16261 aattattgga ctgtattgat atagaaaagg ttatcatggg atggagtgta gatgaagaat
    16321 tatatgtaga aactaaaaaa ctattaaata aatataatgt tgaactatat ctttggttgc
    16381 catgtttttc tgaaatagga ttacttgaaa aaagtaattt actaattgat tatatgggga
    16441 aggaagtcaa gggctatgct ttacaagagg atgaaaattt tgaattttat tgcccaaata
    16501 ataaggaaaa tataaaaaat ataaagaaaa tatatagtac ttattttagt gatattaaat
    16561 ttgatggaat ttttttagat aaaataaggt atggttcttt ttcgaataaa ctaaatggtg
    16621 tttttaattg tttttgtaaa gattgtttaa atatatatga aaaaaataat attgataaaa
    16681 agtcgttaat gagagaaatg aataatgtgt ctaagggagt tatagataat gaagatactc
    16741 catctggtat aacagaatac attaatggaa aatacaaatt taaaaataaa atatgggaag
    16801 atttttttga aacaaaaaat caaaatatat acaagtcatt attagagata agtagttatt
    16861 ttaaaagtaa gggattgaaa ataggtatag atactttttc tccttttatt tcttacttta
    16921 caggtcaaga tttaaaaaaa ttacaagaag tagcagattt tataaaacca atgatgtata
    16981 gaactacaaa agctccagct ggtttaccat ttgaatttga aaggtttatt gatgaatcca
    17041 tacataagaa taaagagatt ggaaaaagaa atttcttaga tattttagga attaaagagt
    17101 atgaaggaga tgtatatcct atagaatttg tactagagga aatcgatttt atgacaagga
    17161 atcttaataa agatatatat gtaggagttg aaataaataa aaaggaaatt atagctcctg
    17221 ttacacctaa gtacataagt gaaaatattt caaatataaa taagacaaca gtaaaaggat
    17281 atgttttatc ttgggatttg ttatcagcac caaaagaaaa tattgatgaa attataaaat
    17341 ggtttcggaa ttagtgaggt gatatagtga gtaagatatt agaaatgcaa caaatatata
    17401 aaagttttgg aaaggtagat gttttaaaag ggataaattt tgagttagaa aagggagaag
    17461 taagagctct tttgggagct aatggagcag gaaaatctac acttattaaa attttaggag
    17521 gagttcattc tcaaactaaa ggtaaagtaa ttttgactgg taatgaaata atgtttaaaa
    17581 attcacatca ttctaaagaa aatggtatta gtataattta tcaagaatta agcttgatac
    17641 caacgcttac agtacttgaa aatatttttc ttggtagaga aattttaaaa ggaagtgtgt
    17701 ttttaaacaa gaaagacatg cttgaggaat atatgaaaat ttgtgaggat tttgatttta
    17761 atatagaccc acatacagta gtttctaatt taagtgtagc aaaccaacaa atggttgaga
    17821 taatgaaagc tgtttcatgt aattctgaaa ttatcataat ggatgaacct acaacatcac
    17881 ttactaataa tgaaaaagat gggctattta aaataataag tagactaaag tctaaaggta
    17941 aaacaatagt ctatatttct cacatattag aggaagtttt taaagtatgt gatagtgcaa
    18001 gtataatgaa aaatggaatt atgattggaa cttatgatgt agaggattta actaaattaa
    18061 agataactca acttatgaca ggaaatgata atcacaaact tattgagaag aaggattata
    18121 catttgcaaa gtatgaagag ccaccagtac tagaagtaaa gaatataagc tggtctaaca
    18181 aaataaaaaa tgtttcattt aaagtttata gaggcgaagt tgtaggtctt gctggtcttg
    18241 taggttcaag aagaactgaa attataaatt tgatatttgg tatagataat aaagatagtg
    18301 gagacatttt tatcaatggt aaaaaagtag acataacttc tcctaaggtt gctataaaaa
    18361 acaaaatagg attgattcct gaagatagaa aaaatctagg attaatttta gaacaagcta
    18421 tatacacaaa ctcatcatca gtacaaattg ataaattcaa gaaatttttg tttatagata
    18481 gaaataaaga aattgagttt tctaaaaaag gtgttaagga attaggaata aaagttagtg
    18541 atgtaaagca agaagtaaaa gagcttagtg gtggaaatca acaaaaagta gtagtttcta
    18601 agtggttaga gaaagattta gatttaataa tatatgatga gccgacaaag ggaatagata
    18661 ttgctgcaaa agaagatatt ttcaatacaa tcaaagaatt ttcaagtgaa ggaataggag
    18721 taatatttat atcttctgat ttagaagaag taataagagt aagtgataga attttagtaa
    18781 ctagagaagg ttctattgta gatgatttaa aaaatgagaa tatacaggtt caagatataa
    18841 tgaacagtat atttaatgta taaaaaatga aaatggagga ctgagatgaa gaaagctttt
    18901 gagaagttaa atttaagaga ttatggagta ttattaggtt ttttagcttt atgtatagct
    18961 ataagtattg caactccatc atttttaggt aaacaaaata tacttaatct tttacgacaa
    19021 tcttctataa ttggaataat ttcagcaggt atgacttttg ttataatatc tggaaacttt
    19081 gatatatcag ttggagcagt agcagctctt gcaggtgcta taacaatgaa atttgcgact
    19141 ggtggaacta atttgtttgc atccatgctc ttaggtgtag cgatttgtgc agtaatagga
    19201 ttaatcaatg gtatagtagt tgcaaagata aatgtaccat cattaatagc aactatggct
    19261 atggtcacta ttgtaagagg attgctgttg atgcttacag gaggatatcc aattactgaa
    19321 aatataccaa tacttgatta cattggaaat ggatatttgt ttaatatacc aataccagta
    19381 gtaattttct ttatagtggt gttagtttca tttattgtac ttaataaaac taagtttggt
    19441 agatatgttt attctgtagg agggaatcaa gaagcaagta aacttaatgg tataaatgta
    19501 gattctcaca aagtgaaagt gtttattatt aatgctgtac ttgcaggaat agctggaatc
    19561 gtactaacag gaagacttgg gactgcaact gctattgcag gtgaaggata tgatatggat
    19621 gcaattgcat ctgtagtaat aggaggtaca tcagtggcag gaggttctgg ttctgtactt
    19681 aaaactgtta taggagtatt acttatgagt gttataaaca acagttttaa tcttcttgga
    19741 atagatgtat atttccagta catatttaaa ggattaataa ttttagcagc agttggattt
    19801 gattcttata gcaagaaaaa acttgcttca agataaaaaa taagggggat ttttttatga
    19861 aaaaattaat gaaacattta gcattgttgt tatcacttgt tatgatattt ggtttagttg
    19921 gttgttcaaa tggtggtgac aaagaaaaaa atggagaaaa gaaaatagca gtattactac
    19981 caggttcaac aggttacttt gtagcaacta aacaaggtat agatgcaaaa tctaaagaat
    20041 taggtgtaag cgtggaatat gcagatgctc aatgggatgc tagtaaacaa ttatcacaag
    20101 ctgaagattt tatggctaaa ggggtagaca tgattgcatt atgtggagtt gattctgctg
    20161 taagtgagag aatagttaaa gctgctaatg attcagacgt ccctatagta gcatttacaa
    20221 attctatagg aagtaaccca actggagaat ttaaaggttt agttacatat ataggacaaa
    20281 atgaggagga aacaggagca ctaactggaa aaattgctaa aaacttatta ggagaaactg
    20341 gtgggaaagc tgtattaata gaaggagtac caggaactac tccacaagta ttaagaaaaa
    20401 aaggtcttga aaaagaatta aaagatagta atatagaaat agtttataat caaacttcaa
    20461 attgggagaa agagcaagct ttaaaagtaa cagaagattt aattcaaaag aaaactgatt
    20521 ttaatgttat aatatgtcaa gatgataatt ctgctacagg tgcaggtcaa gctttaaaag
    20581 atgctggtct taaggataaa gttaaagtca taggtttggg tggaagtaaa gatggattaa
    20641 aagctataac tgatgggtta atagatggta caacttatat gtctgctgta gaagaaggtg
    20701 gattagtcat agaaaaggca tctaaattcc ttaaaggtga aaaaatagaa cctgttacac
    20761 aaattaaaca agttgaagtt aacaaagata atatttcaga gttcaagggt gaatggtaaa
    20821 ttgtcataga taataaataa gatttttaga gttactttag aaactatata aagtaactct
    20881 ttttttaatt taatttagtg ataaattatt gaatagtatt tctaaacaaa taaaaaatta
    20941 atataaacaa agacttaatt atactaaatt ataagtaaaa ctaatataaa cttatggaaa
    21001 aaaggaaaac gtctaaagta ttggaattac tatagtgtag gtgtggtatt aaaaaaacat
    21061 aaaaaaacat aaaaaaattg ttgaaaaaaa tatagaaatt ttgtataatt aaagatttga
    21121 aaagttcggt atacaaagcc aattcttttg tatataatat atataaattc agaaaaagga
    21181 agggatatgt atggccaggc ggttattttt agaaaggatt ggacttatat ttttaggttc
    21241 agcaatatta gcatttggag tttataattt ttattattta aacaacataa cagaaggtgg
    21301 agtgttaggt attttactac tgttaaaaaa tttattcaat atacaaccag caatagcaaa
    21361 tgttgtaata gatggtttac tacttttagt aggatataaa tttttcggta aaaaattcct
    21421 tatatattca atagtagctt ctataacttt ttcagtgtta tatgacttat ttgaagctat
    21481 aggacctcta gtaccacaat cacaaaatat gcttttatca actattttgg caggtgtaac
    21541 tgttggaact ggtgtaggta tagtagtaaa agcaggttgt gcatcaggag gagacgatgc
    21601 tttagcatta gtaatttcta agactacatc tttgaacata gggcaagttt atttagcaac
    21661 agatgttatt gtattgttgt tatcattatt ttatctatca gcgtttgata tcttttattc
    21721 tttgattgct gttacaatta gtggcaaggt aattgatttt atttattatc atggtaaaag
    21781 tttggatatg gatataagta atgatatagt tccagaatgt taatataaat taaataactt
    21841 tagattttat taggggtggc agatttgcca ccttttttgt gtctaaatct ttatagaaag
    21901 tataatccca tctaggtttt tgcaaatctc aaatatagaa ttatgttgga atgactggga
    21961 atacgattgc tagatgtgca ttttaaaaac ccaattttat atttatgaaa tactaatcat
    22021 taatggatgt ttcttaatcc tatctttata cctaaaatga taagataaat gaagtagcaa
    22081 tacaaaacta aaatttgtaa aagagattaa attaaatgga ggagctaaaa tgttggatat
    22141 agttatgata gcattgttat tcataggatt tgtttccatg aagttacttg ctgattggtg
    22201 tgggaaacaa actgagaaaa aataatgtaa tgtatggagg gattacctat gtggtttctg
    22261 gcagttgtaa ttataatgtt aataatatat ttggtatatg cgcttattaa tcctgaaaaa
    22321 ttttaaacag tataaggcta ataagtaagt tgaattgggg ggaacaattt ttatggtgca
    22381 gatagtaata gtgttagcga tttttatgat tttagtaata cctatgggca agtatttata
    22441 tcatatagct actaatcaaa aaacatttgg agatagacta tttgataaag tggacaattt
    22501 tatatataag gtatgtagta tagataagaa aaaagaaatg aactggaaac agtatgcatt
    22561 agctttatta tttacaaatg cagttatggt atttatagga tatatcatac ttagaactca
    22621 aagtatgcat atttttaatc caagtggaat taaaagtatg gaacaaggac tatcatttaa
    22681 tacaataata agttttatga caaatacaaa tcttcaacat tattcaggtg aatcaggact
    22741 ttcatacttt agtcagatga cagtgataat atatatgatg tttacatcag cagcaacagg
    22801 atatgcggct gctatggcat ttgtaagagg tctagtcggt aaaaagaaaa cattgggaaa
    22861 tttttatgta gatttaatta ggataacaac aagagtactt ttaccaggag ctttaattat
    22921 tgggcttata ttagttacac aaggagtacc acaaactttt gctgggacag aaacagtgac
    22981 tactatagaa ggaaagcttc aagatatagc tagaggtcct gtggcagctc ttgaatcaat
    23041 aaaacattta ggaacaaatg gtggtggatt ctttggttca aattcatcac acccatttga
    23101 aaatccaaca ataatatcaa atatagtaga aatattatct atgatgatat taccaggagc
    23161 ttgtgttgtt gcattcggtc acatgataaa gaataaaaaa caaggttggg ttgtgtttgg
    23221 cgcaatgtca atcatatttt taataggtct agtagtgtgc tttaaagcag aaagtgcagg
    23281 aaaccctatt ttatcacaat tagggcttaa tcaaagtatg ggaagtatgg aaggtaagga
    23341 agttagattt ggtatagcac aatcatcatt attcacaaca gtgactactt cctttacgac
    23401 aggtacagtt aataatatgc atgatacact gactccttta ggaggattag taccactttt
    23461 aaatatgatg ctaaatgtag tatttggtgg taaaggcgta ggtctgatga atatgttgat
    23521 gtacgctata atagcagtat tcttatgtgg attaatggta ggaagaacac cagagttttt
    23581 aactaagaaa atcgaaggaa aagaaatgaa gcttattgct ttacttatta tattacatcc
    23641 acttttaata ctaatgttct ctggtctttc agtagcaata ccagcagggc ttgaaggaat
    23701 ttcaaatcct ggattccatg gattatcaca ggtattgtat gagtttgcat cttcagcagc
    23761 caataatggt tcaggatttg aaggtctagg agataacact atgttttgga atataacaac
    23821 aggtatagtt atgttctttg gaagatatgt gtctattata gtactactag caatatcaag
    23881 tttgttagca tctaaaaaag ctgtaaatga aagtatagga acacttagaa cggataattt
    23941 tacatttaca atagttttag tacttgttgt tttaattgtt ggtgctttaa catttttccc
    24001 agccttagcg cttggaccta tttcggagca tctagtgttg tggcactaaa aaattgggag
    24061 ggagtaaagt tttatggaaa ctaaaaaaac aaagtttatt acaaaagata ttttcaaaac
    24121 ttccattata gaagctttta aaaaattaaa tcctaaatat atgatgaaaa atccagttat
    24181 gtttgttgta gaaattggat ttatagtaac attgatactt acaatagtac ctagtttgtt
    24241 tggtgatgaa ggcaataatt taagagctta taatgggatt gtaacaataa tcttatttat
    24301 aacagtcctt tttgcaaatt ttgctgaagc tgtagctgag ggaagaggta aagctcaagc
    24361 agatacactt aagaaaacta gaaaagatac agtagcaagg gttatagata cgttaggaaa
    24421 tgaaaaaatg ataagtgcaa gtgagctaaa acttggtgat attgttttag taaatgttgg
    24481 agaagttatc ccaaatgatg gagaagttat agagggcata gcttctgtgg atgagtcagc
    24541 aataactgga gaatctgcac ctgtcatgag agaatcaggt ggagatttcg catctgttac
    24601 aggtggtact acagttgtaa gtgactggtt aaagataaga ataacaacaa aaccaggaga
    24661 atcgttctta gataaaatga tagctcttgt agaaggtgct tcaagacaaa aaacgccaaa
    24721 tgagattgct ttaaatactt tgttagtagg gttaacactt atatttatgg ttgttttaat
    24781 atctctttat ccaatggcaa actatgcagg tgtaaagatt ccaatttcta ctatgatagc
    24841 tcttttggta tgtttaatac caactactat aggtggattg ttgtcagcta ttggtattgc
    24901 aggtatggat agagtaacta gatttaatgt aattgcaatg tcaggaaaag cagtagaagc
    24961 ttgtggagac gttgatacta tgattttgga taagacaggt acgattactt atgggaatag
    25021 acttgcatca gaatttatag cagttggaaa tgcagattca aaagatttaa taaattattc
    25081 tgttatgtgt tcattaaaag atgatacacc agaaggaaaa tctatagttg atttaggtat
    25141 gaaattaggt tcaacaggta agacaaaaga ggcagaggaa gctgagtttg tagaattttc
    25201 tgcacaaact agaatgagtg gtattaactt agctgatggt agagctataa gaaaaggtgc
    25261 ttatgatagc ataataaaaa gagtaaaaga atctggtggt acagtgccag aagatttaga
    25321 agaaaatgta aatcgagtag ctaaactagg aggaacacct ttagtagtgt gtgtagataa
    25381 tgagatatat ggaattatct acttaaaaga tacagtaaaa tcagggcttg tagaaagatt
    25441 tgctagactt cgagagatag gaataaagac aataatgtgt acaggggata atcccttgac
    25501 agcagctaca attgcaagag aagctggagt agatggattt attgcagaat gtaaaccaga
    25561 agataaaata gatgctataa aaaaagaaca attagagggt aaaatagttg caatgacagg
    25621 tgatggaaca aatgatgctc ctgcacttgc tcaggcagat gtaggtcttg caatgaatag
    25681 tggtacaaca tctgcaaaag aagcagcaaa tatggtggat ttagattcag acccaactaa
    25741 gatattggaa gttgttgaaa taggaaaaca gcttttaata actcgtgggg ctttgacaac
    25801 atttagtata gcaaatgatg ttgcaaaata ttttgcgatt ataccggcta tatttactct
    25861 tgcaatacct caaatggaga tattaaatgt aatgaaactt tctacatcat ttagtgcgat
    25921 attatcagct ttaatattta atgctataat tatcccatgt ttaatacctc ttgcaatgaa
    25981 gggagtaaaa tatagaccta tgaaatcaca agctatgtta atgagaaata tgcttatatt
    26041 tggtcttggt ggagttatag taccattttt aggaataaaa ttaattgatt tattgattac
    26101 acctttagta atgattctta atttaggtta ataaatggag gatataaaaa tgaaaacatt
    26161 aaagccagca ttacttgtaa gtattgtatt gttagttgtt tgtggacttg tatatccact
    26221 tgttttgaca ggtgtaagcc aagttgcatt taaagataaa gccaatggaa gtatgataga
    26281 agtaaatggt gtaaaagtag gttcagaact tattggtcaa agctttacag atgcgagatt
    26341 ctttaaggga agagtatcat cagtaaacta taatacttat acaaaagaag atttagtacc
    26401 agataaagat ggaaatgcat cttatggagg tgtatcatca gggtctttta attatggagc
    26461 tacaaatcca gaacttcatg atagagtaca aaaagatata gaaaagtttt taaaggataa
    26521 tccaactgtc aaaagagagg atataccaac agatttactt acagcatcag gttcaggact
    26581 tgaccctaat ataagtccag cctctgcaaa aattcagata ccagctatag caaaagcgtc
    26641 tggaatatca gaaagtaaat tacaaaaaat tgtagatgat aatacttcca aaaaactttt
    26701 tggagtttta ggtgaagata gagttaatgt actaaaggtt aatatagagg tcgcaaaaat
    26761 attaggtctt atatagtatt ttgtggaatt ttagtaacaa aatattactc aagatttaat
    26821 ttaaaggtta aattttaaag agtgaaaatc aataatgatt ttcacttttt tatttgaata
    26881 ttactaatat ttggaataaa gtgattgtaa gtataaaatc caaaaaaagg ttgcattatc
    26941 aaaatgataa tactataata tatttatcgt tatcaatttg ataatagatt tggacatata
    27001 ggaggaaata gtatgttata taataacgca ttagagttga taggaaacac acctgtagta
    27061 aggctgaata atttaggctg tactaatggg tgttctaaca tatatgtaaa attagaaaaa
    27121 gtaaatccag caggaagtat aaaagataga gctgtttatg aaatgttaga gggcttagag
    27181 caaagaggag aattaaaaaa gggagatgta ctagttgaag caactagtgg aaatactggt
    27241 attgcattat ctatgatagg aaaattaaaa ggatatgaag tagtaatcgt aatgcccgaa
    27301 acaatgagcg ttgagagaag agatttaatg aaggcttatg gagcaaattt ggtgcttaca
    27361 gatggtaaag ctggaatggc aggttccata gaaaaagcaa atgaactttt aaaagaaaat
    27421 ccaaactata aaagcttaaa acaatttgaa aataaagata atccaaatgc acattataaa
    27481 acaacagcag ttgaaattat gaatgatgtc aaagatttag atatattcgt atgtggagtt
    27541 ggaacaggtg gtactttaat aggaactgca agatatttaa aagaacaaaa tccaaacata
    27601 agagtaattg cacttgaacc aaaaaaatct ccagctatat cagaaaacaa gactggacct
    27661 cataaaatac aaggaatagg tgctaacttt gttcctgaaa attatgattc tagtgtagtt
    27721 gatgaagtaa tattagtaga tgatgaagat gcatttgata cagttaaatt acttgcagca
    27781 aaagagggaa ttttagtagg gatatcttct ggagcaaata tatttggtgc tatagagatg
    27841 gcaaaaaagt atccagataa aaagatagtt acagtagctc cagatggagt tgataagtat
    27901 atgtctatgg gaatatttta ataattttat ttgagggagt gaatactttg tttaagaaaa
    27961 taaataaaga tattgaatat ataatgaaga atgatccagc agctaggtct aagatagaag
    28021 tttttttact ttatccatca gttcatgcaa tgataatgca taggatggct catgctcttt
    28081 ataagaagaa gaagcttttt acagcaagat taatatccca gatatccaga tttatgactg
    28141 gcatagaaat tcatcctggt gctaaaatgg gggaaggtat attgatagac catggaatgg
    28201 gtgttgtaat tggagagaca gctgaagtag gtaatagggt gacaatatat caaggagcta
    28261 ctttaggagc tacaggaaaa gatactggaa agagacatcc aactgttggt gatgatgtat
    28321 taattggtgc tggaacaaaa atactaggac ctttaaatat aggttcaaat tcaaaaattg
    28381 gtgcaaattc tgttgtcgtt aaggatgtac ctaatggtgc aacagttgtt ggtataccag
    28441 caaaaatagt aaaaataaga aacctagaac ctgtaaaaaa gaataaaaaa gaagtgtcct
    28501 atgagtatga tgaattagat aatgtatatt atatatagga tttatataac tataaaataa
    28561 actttataaa aattatttta aaaggtattt ttttcaaatt tgaggacata ataaaaaaga
    28621 actgaatgtt acagttttgg ttcatcaatc tttgataaac aagattttaa atttgtagcg
    28681 tctaggagga ttttattatg aaagcaaatg ttaatcaaga tacttgtata ggttgtggat
    28741 tatgtccatc aatatgtcca gaagtatttg atatgaagga tgatgggaaa tcacatgtta
    28801 tagttgatga agtaccaagt gatgctgaag aatcagcagc agaagctaga gaaagctgtc
    28861 cagttgatgc aatagatgtg cattaataaa ataaatgcct ataaaaattg aattttcaat
    28921 ttttataggc atttgtcttt tattcagtta gtatttcaac ccctgtttct gtaactaaaa
    28981 ccatacattc ccattgagca gataatttac catcttttgt ataaatagtc caattatttt
    29041 cttcatcaac aaaaatttcg tgagttcctt catttaccat aggttctatt gtgaatatca
    29101 tcccaggaac caatagcatc tcagtccctt ttttagatac ataacttaca aaagggtctt
    29161 catgaaagct taatccaact ccatgaccac ctatttctct aacaacagag tatccatttt
    29221 tctttgcata agagttgata gcatcaccta tatctcctaa aaatccccat ggtttggcaa
    29281 cttcaagacc tttttggaca cattcctttg taacttgaac taattttttt gcattttcat
    29341 taacattacc tatcataaac attcttgaag catctgaata gtatccatta aaaatagtag
    29401 aaacatctac atttataata tctccatctt ttaagataat gtccttgctt ggaataccat
    29461 gacaaacttc attatttact gatgtacata cacttttagg aaatccatta tagtctagtg
    29521 gagcagggat agctccattt tgtagtgtaa agtcatgaac caacttattc atatcttctg
    29581 tagacattcc aatttcgatt ttatctgaaa tataatccaa aatttttgtg tttattacag
    29641 cacttttttt tatttcctca atttgacttt cattttttat aatatcatga gttggtacaa
    29701 tatgacctaa cttagcaaat gaatctattt tattatccat atccatatga cattttttat
    29761 attttttagt acttccacac caacagctat catttctatt taacttcata acttttcatc
    29821 cttcttttat tatttattta aattatgggt tacataaact attttaaaac ataaccatta
    29881 taaatattat aacataaata ctaagaaatc ctttatagat agtgatttat tcaaagtata
    29941 aattattata tggttattgt tgatgaaaag aggatagtta aatgtattat tggatattag
    30001 cgcacttaat agtttagttc aagtttttca agaaaatcat atgttagagg acaattttcc
    30061 tttaaagtat tttttgaatc ttcatcaaga taataatgca caaatgattc tgcaaaaaat
    30121 tcttctataa acttgcttat atatgcatgt gcaacttgtt tagggtcata aagcttatat
    30181 ctttcctgag caaatacttc cataaattct tcagaatttg attttttatt taaaacattt
    30241 ttatctatag catgtgcagt ttcatgtaat tctaaatttt ttgatgaatg tacatttgca
    30301 tagctagggt tactataacc tattttagct actatagaat tatttctata tataccaggt
    30361 acatctttcc aagtcttgcc tgatcttacc caacatggag gtaattgata ttttaaatca
    30421 ctaaattctg gttcatcagt tagatttgaa tttattaatt ttatttttat atccttttct
    30481 ttaagtgtct gtaaatattt aatatttatt tttgcaagtc tgcttttcat attttcaact
    30541 tcatctttat cataattcaa gtcatcagga agtataagca tttgttcaat tatatctatg
    30601 ctactgtctt ttatatcttc agattcttca tcaaatttat attgaagagt attattttca
    30661 tagatagtta tatttgtatt attttccata taataagagt gaatatcctc taagtctaaa
    30721 atattcattt gttgtaaaat atgttttgtt tcgccactcc atactactaa agaaagtgaa
    30781 ttaaaattaa ttagtggatt ttcctttgct tttatatcat tgcttatatc tacatcaaat
    30841 acatattcaa ttgagtcttc tatgttaaga ctatctgtat tctttttaat atataaatat
    30901 gcttgtttta agtctatata aatctcatct aaagattcat cataaaattc tgataaatta
    30961 ttagtaaaga gttcttcact taaaagttca tttttatcag ttatactttc aattaaaata
    31021 ttaatttctt ttatcatatc gtaaatactt atttttctat ttgctttttc actagataca
    31081 ttgcatggcg caagagcaat gcaataaaaa tctttttctt ttttataaaa ggctttaagt
    31141 gattttgata aaagtgtaaa gtctacacct atctgtaagt tgaccttaag tgagtcattt
    31201 tgtaataatt cagtattatt ttttttagct atatttaaat tgttttccat aatcattgcc
    31261 ccctttttat tagggtatca taattttttt atatgtgtca atttgttttt ttgaaaatta
    31321 taattttttt gaaaatttta ttttttataa tttgtgaatt ttttattata gaaataaata
    31381 tgttattatt atgtcaaaat ttagcattag attcatgtag agtatgtttt atacatatat
    31441 tataattaat tctttgaaga ttgcatattc aaaatacaca ccattttttt taattctata
    31501 atataatatt tatatattta atgttaggat gtgatttaag gtgtatttta catatattat
    31561 aagatgtaaa gatgattctc tatacactgg atataccagt aatatagtta gaagaatgaa
    31621 tgaacataag ttaggaatta attcaaaata cacaagagca aagggatttg aaaaactaga
    31681 agtttatttt gtgaccaaca caaaaagcaa tgctatgaag ttagaatact atataaaaaa
    31741 attaactaga aataaaaaat tgtctataat aaaaaaccca agtatactta taaatttaat
    31801 tgataataaa gaagattata ttattggtaa ggaaatagaa caattaacat aaatatttat
    31861 agtaacatta taatgggtgc atatagtgta aaaacttgac aataaatact attgtaatat
    31921 aatgaagtta taaatatagt aaattaaaat agctagggga gccaagagct gagaggtata
    31981 ccgaccctta cacctgatct ggataatacc agcgtaggaa agcttagtaa aaattcaagt
    32041 atgtattatt tgtgatatat gctatttcta agctatacct attgtaggta tagctttttt
    32101 aattaagtta tttaaattaa actttaggag gcaacaatga gtaattataa aataccaaca
    32161 cttacaatag ctggctctga ttcatctgga ggagctggga ttcaggctga tttgaaaacg
    32221 tttagtgcaa ttggtactta tggtatgagt gtaataacag ctataacagc acagaatact
    32281 aaaggagttt ttgctgtaga ggatttaaat aagaaaataa taaaaaaaca aatagaggca
    32341 gtctttgaag atatacctcc aagagctgta aaaataggaa tggtttcaag tcctgagatt
    32401 atacttgaaa tagttgagaa tctaaagaaa tacaatccaa aatatttggt tgtggaccca
    32461 gtaatgatat ctaaaagtgg atattatcta ttaaagccag aggcaaaaga aaacttaatt
    32521 aagtatttga taccacttgc atacataatt acaccaaata taccagaggc agaagaaata
    32581 acaggaataa aaatacataa tgtagatgat atgaagagag taggagaaga aatactacaa
    32641 cttggaccta aatttgtact tatgaaagga ggtcatttag atggagaagc agtggatatt
    32701 ttagttggta aaaatatatt taaagtttat aaaagtgaaa gaattgataa aaaaaataca
    32761 catggaacag ggtgcacatt atcttctgca ataacatctt atctagcatt aggatatgaa
    32821 ataacagaag cagtaaatct aagtaaaatc tatataacag aagcaataaa aagaagtttt
    32881 gatataggtc atggagtagg accagtacat catttttata aatttgagta ataaaaaaat
    32941 attggaggta gaatatgtat aatttaatta aagatgtaaa aaaattaaat ccattggtaa
    33001 ttcattatac taataatgta actataaatg attgtgctaa tgtgacttta gctgtaggag
    33061 caagtcctct tatgagtttt tcatatgagg aagtagaaga aatggtaagt gtagcaaatt
    33121 ctgtagtaat aaatatagga actatgaact caaatatgtt ggatttattt ctattagctg
    33181 gaaaagcagc aaataagtac aataaacctg ttgttttgga cccagttgga gtatttgcaa
    33241 gcaaagcgag agcagaactt acaagtagac ttttaaatga agtcaaattt agtgttgtta
    33301 aagggaatgt atcagaaata aagtttatag gtggctttaa tgtaaggggt aaaggtgtag
    33361 attcatttga tgaagaagaa gattctactg agataataag aaagattgct gaaaagcttg
    33421 aatgtgtagt tgtagcaact ggaaaaatag atataataac taatggtaaa ggtatatata
    33481 aaataaacaa tggaactgat aaattaaaag gaattacagg aactggatgt atgactgcta
    33541 gtttgattgc aagttttatg gctgttactg agaatatatt agaagcagca acaatgggtg
    33601 ttcttacaat gtctttaagt ggggaattag ctaatttaaa taatccacca ataggtacat
    33661 ttaaagaaaa tcttatgaat gctatttatc aaatggatat agatacttta agtaaaaatt
    33721 ctaatattga atttttaaat taataaagag taagggagac ttaaaatgat tgataaagaa
    33781 tctttaaaaa aatgcttaaa gttgtatttg gtaactgact ctgaaatgct taaaggtaga
    33841 gacttttata aatgtcttga agatgcaata tcttctggaa taacaactgt acaacttaga
    33901 gaaaaaaatg cttctggaag agagttttta agaaaagcta tgaaacttag agaaataact
    33961 gaaagatatg gtgttaagtt tataataaat gatagagtgg atatagcact tatttgtgat
    34021 gcagatggag tacatgtagg acagagtgac attgatgtga gagaggttag aaagcttata
    34081 ggaaacaata aaattctagg agtatcagca agaactttag aagaagctac ttgtgcaaaa
    34141 aatgatggcg cagactattt aggtgtaggg tctatattca caacatctac taaattagat
    34201 gcaaaatcag cttcgtttga aactgttaaa gaaattaaag aaaaagtaga catgccattt
    34261 gtgctaattg gaggtataaa tttagacaat atagataaac ttaaatgttt agaaagtgac
    34321 gggtatgcta taatatcagc tatattaaaa gctgaagata tctctaaaga agtggaaaag
    34381 tggacattaa aaatataaaa ttggctctgg tagtgaaaaa aatggaaaaa tttgatataa
    34441 tctaagagta aaatttaagc aattataaat gataaacttt tctacatata ctttttaatc
    34501 aagatatatg tagatttggt taaaaaggag ctataaatga ttaagatatt gttagtgtgt
    34561 gttggaggca tgtcttctac cctattggta aataaaatgg agaaggatgc aaaaaaaaga
    34621 aatatagact gtaaaatatg ggcagttgga gaaggagata taaaatcgga gcttgataac
    34681 tttgatattt tacttcttgg accacaactt agatttatgc ttgatgacgt aaagtctata
    34741 gttggagata gagttccagt atctataata gatatggtaa actatggtac ttgtaatggt
    34801 catgctgtgt taaattcggt tttagaaata ttaaaataaa taataaaagt ggattctata
    34861 taatagagtc catttttatt atttgttatt aataggctta aaacttagct taccatttct
    34921 ttttttaatc tccacatagc aagtgaaaaa cttgaacagc acaatattaa aaatagtagg
    34981 ataacaggca gtatatctcc aataagaata gtctcaccaa atattaagct acgtaaagcg
    35041 ttaataacat gggtaaatgg attgagcatc actgctactt ttaatcctcc agataaactt
    35101 tctataggga aaagagctga acttaaaaag aaaataggaa gtacaattgc agtcataatt
    35161 gtttcataaa ctatttcatt tggcaataat aaacttatag aataagctag acttgataaa
    35221 aagaaggctg tcataaatat cagtactatc ataagtagga ttccaccaat gccagattct
    35281 atccttactg agaagaatat acttactata cacaaaatag ttacttctat gaaggaaact
    35341 aagatagctt ctaataattg accaaggacg attgaatacc tactgatagg tgcaattaag
    35401 actctataga aacttccact gttcttcatt ataaagttta taataccacc actactacaa
    35461 gaagaaaaca caaccagtac aatgatacct ggtagtataa aagcagtata attattgata
    35521 tttatattat tcatagtttg gtttgcaata gaactataaa gcacaagcca aagaaaaggt
    35581 tgtaatatag taactacaaa tgagattgga tttttaaaac gccacttcat actacgccat
    35641 aagatattta acattcccat tcctccttta agcttttttt agaacttgta agtgctaaaa
    35701 atacatcttc gagacttggc tctacaattt ctattgcatc aaattctata tcatgttcta
    35761 aaagccattt atttattaaa gtaaaagcag ttctactatc gtttacactt ataaaaattg
    35821 aattttctcg tacgctcata aactttacta aaccagtact tttaattgaa tctttatatt
    35881 tttttatatc ttcagtatta tggaaagtta tacgtagtat attttgtcta atgtaactac
    35941 gtaaacttga tggtgttcct tgtgctaaat cctttccatt cttcataata caaatattat
    36001 cactcaattg ttcagcttct tcaagataat gagtagttag aaatatagtt gtgccgtact
    36061 catctctaat ttttaataac atatcccaca tagattttct tgaatcgaca tccataccaa
    36121 cagttggttc atctaaaaat aggattttag gagatgatac catattcata gctatgtcca
    36181 atctacgttt tacaccccct gaatatgatg aagtaggata ttttaagtaa ctagataagt
    36241 caaacttatc aattaaagag tctattcttt gttttgcaat ctgtggttct actttataca
    36301 ttttactttg aaataccata ttttccataa gggataaatg ttcatcaata gagatttgct
    36361 gtgctacaca agcaatttga gtacgtatcc aagaaggatt atctactaaa tcttttccaa
    36421 acatttttac atttccagat gtaggtttgt aaaaggtagt caaaatattt ataagagaag
    36481 atttaccagc accattagga ccaagtaaag aaaaaatttc tccagcattt acattaaagc
    36541 ttagaccatt taaagctttc acaccatttt tatattcttt tactaaattg tctatttcaa
    36601 tcgctaacat atattattcc tcctctttaa gtggaaatat tatctctgta atatagttat
    36661 ctgggtttcc ttttatcaac atacctggtc ctttaataaa tacttctctc caaggagttt
    36721 gtatttttat attattttca tgaatatatt taaatatcat ttcatacgtt tttggcaaat
    36781 tactataaga gcctgtgtga gtaaaaaata aagcttttgt tttaggaaat tcttttgtag
    36841 ttatacccat acgatttgga gtttcagcag ttggaacaca taattccatt tctgcaacac
    36901 cagttttttg gtcaacatca tagtagcaaa agaatggtgc accgttagac tttcctttta
    36961 tagctttaaa tacatttgga aaatactttg ttgcctcagt cataggtcca ttataacgca
    37021 tggtagccac tgtgactgat tcgatatttt tgatttcaat tttgcaattc atttgtttac
    37081 ctcctaatgt gtgttcatat tcttactaga tttttaagca ttaatacata taagattttg
    37141 attagttgaa aacatataat cttatctcgt ttgcttgttt tcaagtctaa cataacataa
    37201 aatatatatc ctctcatttg atgctccatt ttgtaaagct attatttaag ctttacttta
    37261 gattttttag caaattgaaa taaagcaatt tataaaataa gtatttacaa attaagtgta
    37321 ttaatcgtat aatacactta ataggatact agataaagtc tgaaataaag gcgtattttt
    37381 ttaaagtaag tgtactatcg tattaataca ctagaatagg aggaatcatg aattttgaat
    37441 tagataatac aactcccatt tatttgcaaa tagtaaagta tataaagaga caaatagtaa
    37501 caggagagtt gaaaccagga gagacaatac catcaagaag agaaatggca cttaatctaa
    37561 aagtaaactt aaatacagtt caaagagcat ataaggagat gggtgatatg aatataataa
    37621 atacttttaa aaattatcaa agcagtgtaa cagttgatga aaatatatta aaaaatttaa
    37681 aattagagct tataaatgaa tcactatctg tttttataga agatatgaaa gcaataaatg
    37741 ttagcaaaga agaagtttta aaaataatag aagataagta ttagtttttt aacaaataga
    37801 aaacttagtt tagaaagttt atttataagc atattggatg tggaggatag taaatatgat
    37861 agaaataaag aatgtcagca aaacatataa aaggatgcaa gggcttaaaa taagaaagat
    37921 agaagcttta aaaaatgtaa gttttaatat agaaaaggga aaaataactg cattacttgg
    37981 tataaatggt gttggtaaat caactatgtt aaaagcaata gcaggactta taaacattga
    38041 tagtggagaa attcgtatag atggagaaaa aataaatgaa aaggtataca ataaattggc
    38101 ttttgttcca gatgtacaat ctcactttag caatacaaca ataaaagaaa catttgaatt
    38161 tatggaaatt ttttattcaa agtggaataa agaaaaatca aaagaaatga tggacatatt
    38221 taaattggat gaagatgaaa taattgataa cttgtcaaaa ggaaacattg caagagtaaa
    38281 attaatactt ggattttgcc aagacccaga gtacatactt cttgatgaac catttacagg
    38341 aattgatttg tttaagaggg aagagtttat aggagtaata gcgcaatata tggaggaaaa
    38401 tcaagccatt atcataacta ctcatgagat agtagagata gaaagtttag ttgatgaggt
    38461 tgtaattctt gatgaagggc agattataac ttcatttaat gcagaagaat taagagaaag
    38521 agaaggtaaa tctatattag ataagatgag ggaggtatat aagaatgaat aaacttctat
    38581 atctaataaa tgtagatttg agaagaagca ataagtttta tatagcttat atatcatttt
    38641 tttcattaat tacacttagt ttaaatttat tcgaaattaa taaatttaaa aattctaaat
    38701 ttattatgaa cttagcaata gaagattttg gaggtatatt t
//
LOCUS       DAEPLF010000017        37698 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEPLF010000017 DAEPLF010000000
VERSION     DAEPLF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.017"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(90..1244)
                     /gene="nagA"
                     /locus_tag="KR314_000760"
     CDS             complement(90..1244)
                     /gene="nagA"
                     /locus_tag="KR314_000760"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBH0327160.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNITDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQRKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFSVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYVPALSCNIDNVCGSIHQGMDADFIVTDKNFNIYETYISGICMYKVNK"
     gene            complement(1327..2052)
                     /locus_tag="KR314_000761"
     CDS             complement(1327..2052)
                     /locus_tag="KR314_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0327161.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRIQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            2321..3250
                     /gene="pfkB"
                     /locus_tag="KR314_000762"
     CDS             2321..3250
                     /gene="pfkB"
                     /locus_tag="KR314_000762"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBH0327162.1"
                     /translation="MITVITFNPSIDRMYKVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEYNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            complement(3379..4296)
                     /locus_tag="KR314_000763"
     CDS             complement(3379..4296)
                     /locus_tag="KR314_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBH0327163.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     ICSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVNQ
                     GEELIELSKKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKHFSVNTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            4758..5927
                     /locus_tag="KR314_000764"
     CDS             4758..5927
                     /locus_tag="KR314_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0327164.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     NRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPQGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            5976..6827
                     /gene="gatY"
                     /locus_tag="KR314_000765"
     CDS             5976..6827
                     /gene="gatY"
                     /locus_tag="KR314_000765"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBH0327165.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            7153..9054
                     /locus_tag="KR314_000766"
     CDS             7153..9054
                     /locus_tag="KR314_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0327166.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASNLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            9057..10100
                     /gene="ypdE"
                     /locus_tag="KR314_000767"
     CDS             9057..10100
                     /gene="ypdE"
                     /locus_tag="KR314_000767"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBH0327167.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            10115..10420
                     /locus_tag="KR314_000768"
     CDS             10115..10420
                     /locus_tag="KR314_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0327168.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPTMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            10517..10852
                     /locus_tag="KR314_000769"
     CDS             10517..10852
                     /locus_tag="KR314_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0327169.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            10884..12206
                     /locus_tag="KR314_000770"
     CDS             10884..12206
                     /locus_tag="KR314_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0327170.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            12216..13286
                     /locus_tag="KR314_000771"
     CDS             12216..13286
                     /locus_tag="KR314_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0327171.1"
                     /translation="MNNTIERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            13291..14478
                     /locus_tag="KR314_000772"
     CDS             13291..14478
                     /locus_tag="KR314_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0327172.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVANNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(14675..15547)
                     /locus_tag="KR314_000773"
     CDS             complement(14675..15547)
                     /locus_tag="KR314_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBH0327173.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVSYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYKQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLHLNPEVFKNDALIYTNIDEVSIYNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(15941..16132)
                     /locus_tag="KR314_000774"
     CDS             complement(15941..16132)
                     /locus_tag="KR314_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327174.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            16352..17398
                     /gene="cobT"
                     /locus_tag="KR314_000775"
     CDS             16352..17398
                     /gene="cobT"
                     /locus_tag="KR314_000775"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBH0327175.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            17430..17990
                     /gene="cobU"
                     /locus_tag="KR314_000776"
     CDS             17430..17990
                     /gene="cobU"
                     /locus_tag="KR314_000776"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBH0327176.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            18000..18773
                     /gene="cobS"
                     /locus_tag="KR314_000777"
     CDS             18000..18773
                     /gene="cobS"
                     /locus_tag="KR314_000777"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBH0327177.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWIIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            18793..19407
                     /locus_tag="KR314_000778"
     CDS             18793..19407
                     /locus_tag="KR314_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBH0327178.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      19474..19653
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            19783..21282
                     /locus_tag="KR314_000779"
     CDS             19783..21282
                     /locus_tag="KR314_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBH0327179.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVGVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            21321..22697
                     /locus_tag="KR314_000780"
     CDS             21321..22697
                     /locus_tag="KR314_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBH0327180.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            22698..23669
                     /locus_tag="KR314_000781"
     CDS             22698..23669
                     /locus_tag="KR314_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBH0327181.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYVSSFVSMLVFTLIHVLCRMILINLII"
     gene            23701..24771
                     /locus_tag="KR314_000782"
     CDS             23701..24771
                     /locus_tag="KR314_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0327182.1"
                     /translation="MKDLGHGANVGEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIGINPDFIIPGNGATEVIYLLMKSVKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLGKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRAYKKTTKELKKDLLKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            24773..25660
                     /locus_tag="KR314_000783"
     CDS             24773..25660
                     /locus_tag="KR314_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBH0327183.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSEVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVIILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDTLCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            25648..26280
                     /locus_tag="KR314_000784"
     CDS             25648..26280
                     /locus_tag="KR314_000784"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBH0327184.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            26289..27515
                     /locus_tag="KR314_000785"
     CDS             26289..27515
                     /locus_tag="KR314_000785"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBH0327185.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCKERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            27512..28120
                     /locus_tag="KR314_000786"
     CDS             27512..28120
                     /locus_tag="KR314_000786"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBH0327186.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNVVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKIPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            28110..28685
                     /locus_tag="KR314_000787"
     CDS             28110..28685
                     /locus_tag="KR314_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBH0327187.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            28678..29430
                     /locus_tag="KR314_000788"
     CDS             28678..29430
                     /locus_tag="KR314_000788"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBH0327188.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            29414..30535
                     /gene="cbiG"
                     /locus_tag="KR314_000789"
     CDS             29414..30535
                     /gene="cbiG"
                     /locus_tag="KR314_000789"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBH0327189.1"
                     /translation="MNIEGNIAIICITENGKNLAVKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIIFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSIDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPIAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            30532..31257
                     /gene="cobJ"
                     /locus_tag="KR314_000790"
     CDS             30532..31257
                     /gene="cobJ"
                     /locus_tag="KR314_000790"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBH0327190.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            31254..32006
                     /gene="cobK"
                     /locus_tag="KR314_000791"
     CDS             31254..32006
                     /gene="cobK"
                     /locus_tag="KR314_000791"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBH0327191.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            32025..33503
                     /locus_tag="KR314_000792"
     CDS             32025..33503
                     /locus_tag="KR314_000792"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBH0327192.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEEDGFDVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            33533..34438
                     /gene="hemC"
                     /locus_tag="KR314_000793"
     CDS             33533..34438
                     /gene="hemC"
                     /locus_tag="KR314_000793"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBH0327193.1"
                     /translation="MNIVVGTRGSTLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            34428..35927
                     /gene="cobA"
                     /locus_tag="KR314_000794"
     CDS             34428..35927
                     /gene="cobA"
                     /locus_tag="KR314_000794"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBH0327194.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEDIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDNDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEDKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            35944..36909
                     /gene="hemB"
                     /locus_tag="KR314_000795"
     CDS             35944..36909
                     /gene="hemB"
                     /locus_tag="KR314_000795"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBH0327195.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     NSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            37062..37640
                     /locus_tag="KR314_000796"
     CDS             37062..37640
                     /locus_tag="KR314_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBH0327196.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        15012 a         3971 c         6724 g        11991 t
ORIGIN      
        1 gtatgaaatc aaatgtattt tcatcatttt aacaatgaaa aagagactta tctctatttt
       61 taggaagcct ccataacttt atattgaatc tatttattaa ctttgtacat acatatacca
      121 cttatataag tttcgtatat attaaagttc ttatctgtta ctataaagtc tgcatccata
      181 ccttgatgta tggagccaca gacattatca atattacaac ttaaagcagg aacatatgta
      241 gccattttta ttgcatcttc taaagtggct atgttccaat caactatatt tctcacagcc
      301 ttgtccatag ttaatacact tccagctaaa ctcccactat ttaatcttgc aacactattt
      361 tttacattta cactaaactt accaagagta tattttccat cctccatagc accagccctc
      421 atacaatctg taattaaagc tatgttatta ctagacttcg catccatcat tattttaaca
      481 gctattgggc ttacatgatg accatcacaa attaattcag cataagtatc ttttagtacc
      541 atagcagccc caaccattcc aggttctcta tgatttaaag ggctcatccc attatatgta
      601 tgtacaaaca ctttagcacc tgaattaacg acttgtttaa cttgttcaaa tgtagctgaa
      661 ctatggccaa gagaaacaga gatattattt ttagttgcat atcttgttaa ttctattgaa
      721 cctttacgtt ctggagcaat tgctattttt cttattaaat ttttcgattt tctttgccat
      781 aattttaata tattgatgtc agggtcttta aaatattttt cattttgagc gcccttatat
      841 ttttctgtaa aaaatggacc ttctaaataa atcccttgta tttttgcacc ctcaactcga
      901 ttgaagctat tgcttatatt ttctacagaa tcacttagtg tatctacaga atcagttaaa
      961 gttgtaggta ggaaagatgt tactccatat tttaataagc cttttgaaat tgtgtttatt
     1021 gcttcaaatg tattgtccat gacatctgca cctaaaagac cgtgaatgtg agtatcaact
     1081 aagccaggag caatatattt acctgaaaaa tctatgacat tatcagtaat attaggtctt
     1141 tcttgttgaa attcaccgaa tttgccatta attatactaa ggtaaccttt ttctttgatt
     1201 ccatgtttca taaaaaattt atctgcataa ataaaacttt tcaaaataac ctccttataa
     1261 ttatactatc attgtggcat tgacgtttat aatatcattc tattttcttg aaagctattt
     1321 aattcactat cttacatgtc ttactctata tacaaattta tcgcttctag caacactcaa
     1381 agtaaattca ataactatat tgtcatcatt atatgtggta cgaagtaatt tcagacaagg
     1441 tgaatctcct ggtacttcca ataacttagc ttccttatca gagattattc cagctgaaaa
     1501 ttcttcatca gcaactttaa taatctcgcc ataatcttct ctaaatattt catataaagg
     1561 tttttgttcg attttttctt ttgtcaatcc tataaattct ttcataggaa gatatgttct
     1621 ttcaagcatc ataggaaggt catcagcaag tcttaaacgc attattttta tcattttttc
     1681 accaggagtt atacctaatt tttcagagaa atactttgta ctttctacta cctcaaatga
     1741 taataccttt gtacctggct tttttccttg tattctcata taatctgtaa agctataact
     1801 ttccattaag ttttgagaat tttgacttag tgcagcaaca aaagttcctt ttccatgtct
     1861 tttgtatata tatcccatat gttccaactc agccatagct agtctaactg tggttctact
     1921 tactccatat tcatcacaaa tttttctttc agaaagcatt ctatcgtttg gttccatctc
     1981 attttcaatc ttagttctta atatctcaac aagctggtca tatattggtc ttttatcatt
     2041 tctttttatc ataaaaacat agtcctttct aagtggttat taccacaatt tgattataga
     2101 atatagaatt atttatgtca atgaataaag tttaatcaaa attcaataag taaggctatt
     2161 aatttattat aaaattatgg tatattgaca aaacaagtaa aataatttat attcaaattg
     2221 gtaataacca ctttgggtat attatagatg aggtattttt tattttacag attaatttat
     2281 tgtttagaaa agtaaaaatt aataatgagg agaatctagt atgattacag taataacatt
     2341 caacccatct atagatagaa tgtataaagt gaataatata aacataggtg aggtgcaaag
     2401 ggtagtaagt aagaatgcaa ctgctggagg taaaggatta aacgtagcta aggtatgtaa
     2461 aattttaaaa gaaaatcctt tagctatggg atttctaggt ggatttaatg gagaatttat
     2521 aaaaaatgaa cttagaaaac tagatataca aaataaattt acaaaaatag agcaggaaac
     2581 tagaaattgc ttaaacataa ttgaggacaa taaagttagt acagaatttt tagaaaaagg
     2641 accaatagta gaaaaaagcg atttagagag atttgaaaat gatatagagg aagtactaaa
     2701 aaatactaaa atactagtag cttctggtag ttattgtgag aatatgccga tagattatta
     2761 tgaaaagata ggaaatatat gtagagcaaa taatataaaa tttatattag atacatcagg
     2821 agaagcatta aaaatagctt taaaatcaaa accatattta ataaagccaa atatagatga
     2881 aataagacat ttattaggaa taaatataga atcaagagag gaaattatct tgtcaggaaa
     2941 aaaacttatt gaaatgggtg cagagaatgt atgtatatct ttaggaaaag atggtatggt
     3001 gtatttaaac gaatataatg tatatgatgt aaaagtacca gttgtaaagt gtataaatac
     3061 tgtaggtagt ggagatagca cagtggctgg atttagtgta ggtattttaa ggggttataa
     3121 aatagaaaat ttacttaaat ttgcaaatgc ttgcggtata agtaatgctt taaatatgga
     3181 aactggattt gtagatttgg gagaaattga taaatatcaa gatttagttg aagtaaaaaa
     3241 gttaagttaa ataaatttaa tgggaatata ataaatttaa tgttgttgta atagatttta
     3301 attttaatat aaaagatata gtcttatata aaaataggtt agaaaaattc atttttaacc
     3361 tatttttata ataaatatct attttactga atttataatt ttctctagta atctttgagc
     3421 cttgatacac tcttctccat taatagctgg ttttatatta ttttctatac aatcaataaa
     3481 atgatttgat atatcctcaa aacccttttg ttttaatata tttacccatg caccactttg
     3541 ttttaaagtt aggtttccac cttcttctat ttccagtaca ttaagattct taactctttg
     3601 aatcttacct tttgaaagaa tttcaacttg ttctaacttt gttccagaat ctctatgcat
     3661 agaggtgttt acagagaaat gtttaccttt aagtttatga cttacaaata taagattctt
     3721 attgtctgtt aaaaataaat cttctccgct aatttcaaca tcttcatttg ctaaccataa
     3781 agcagtgtca ataacatgta tatagtcatc tataagggta ctgtcgaatc taacatttct
     3841 taaggaattt aatccatgtt tacatatatt gatagatacg atttctgtag cattattttt
     3901 aatttctttg tacataggac agaatctacg gttgaatcca accattaaat ttaagttttt
     3961 tttcttactt aactcgataa gttcttctcc ttgatttact gttgaagcaa gaggtttgtc
     4021 tacatataca tgaacaccta aatttaataa tatttttata atttcgtagt gtgtttctgt
     4081 agagctatgt agaaaaatac aatcacattt tttagctagg gattcaatag aatcgaatgg
     4141 cataattcta taatcactac agattttttc ccttttaact ttatttggag taaaagctcc
     4201 tacaaactca aatctctcag acttagttaa tataggtaaa tatgcctttt gagctatgga
     4261 gccaagacct atcattccca tttttatatt tttcataata ctttctccta aggtttttat
     4321 atttacagaa ttgattataa ttcagacaca taaatttgta aactttgtat caaaaaataa
     4381 atttaaagct tatataagta aattaacgaa ataagaaata ttgacaaagt aattttgtag
     4441 gatatctata aaattgattg atataaatat tttatgtatt gacaaaaaat tcaaattaat
     4501 ttatatttgg attagtggta attaccactt tggtctgaac aagtttttat cgtaaaataa
     4561 aaatactaaa gtatgatttt attaaatttt taaagataaa ctataaaatt aattaaaatc
     4621 tatagcgtat attttatagt gagtttaagt agtactaaaa aatctaagca aaaaattata
     4681 aaagagtata tttttttata gtttaaagtg gttataacct cattaccaaa tgtaaacgtt
     4741 acaagtatga aaggagaatg aaaatgttta aattagaaga acaaaaacta aaagatttag
     4801 gtgcaataat aacaactaat gaaataaaac aacaacctga gttatggtta gagacttatg
     4861 aaatatataa atctaacaaa gaaaaattaa atagattcat agatacaata agtaataatc
     4921 atgggcagtt tagagttata tttacaggtg ctggaacttc agcttacata ggaaattcaa
     4981 tacttcctta ccttaagaat aaaaatgaca taagaaaata catatttgaa gcaataccta
     5041 ctacggatat agtttcaaac ccatatgatt acttaaaaaa agatatacca acattattaa
     5101 tttcatttgc aagaagtgga aatagtcctg aaagtttggc tgctttaaat ttagggaata
     5161 agattgtaga taatttttat catttagcta taacttgtaa tccgcaagga gaactagcta
     5221 aaatgactaa aaacgacgaa aataattact tgttattaat gccatcaaaa tcaaatgatg
     5281 aaggctttgc tatgacagga agtttttcgt gtatgatgtt atctgctatg ttaatattcg
     5341 atagtttaga agatgatgta gaaaagtcat atataaatgc aataattgaa atgggaagaa
     5401 atgttataga tagaaaagat gaaatacatg agttaataaa taaagatttc gatagagttg
     5461 tatatttagg ttcaggagga ttaggtggtt tgacacaaga agcacaacta aaacttttag
     5521 aacttacagc aggaaaaata tctacggtgt atgattctcc tatggggttt agacacggac
     5581 caaaatcatt tattgatgaa aatacacttg tatttgaatt tgtatctaac tgtttatata
     5641 caagaaaata tgatttagat gttttagagg agataaaaag ggataaaatt gctaaattca
     5701 cttgtgcagt gtcagtagaa aatgaaaata acttctcagg aacaaagttt gagtttaaag
     5761 aaaagtataa taaattacca gatgtgtatc ttgcaatgcc atacatattg tttgcacaaa
     5821 ctatagcatt gtttgtatcg gtaaaggttg gaaataagcc agatacgcca tcagctacag
     5881 gaacagtaaa tagagtagta aaaggtgtaa caatatatga atattaaaag ataagattaa
     5941 aatattataa ctaaataatt tgggagggta tcaatgtggc attagtaaca acaacatcaa
     6001 tgcttaaaaa ggcacaagat gaggggtatg cagtaggagc atttaatgta gaaaatatgg
     6061 aaatggtaat tgctgttata gaagcagctg aggagttaaa ctctccagta atattacaaa
     6121 ctactccatc tactgtaaaa tatgcaggtc ttgattatta tctagcaaat gcaaaggttg
     6181 ctgcagaaag agtaaagata cctgtagcaa tgcatctgga ccatggttca agttttgacc
     6241 ttgcaatgaa agctttaaga agtggatata catcaataat gatagatggt tctcatgaaa
     6301 gctttgaaaa taatatatct atatctaaat cagtagtaaa agcatgtagt ccttctctta
     6361 taccagtgga agcagaactt gggaaagttg gtggaaaaga agatgattta gatggcggga
     6421 atagtaatga ttatacaaat ccaaaagagg caaaagagtt tgtcgataga acaggaatat
     6481 cttcattggc tattgctata ggtactgcac atggattgta taagggtgaa cctaaaatag
     6541 atttagatag gttatcagaa attaaaaatg tagtttcaat acctttagta ttgcatggag
     6601 gttcaggaat acatgatgat attataaaag aagctataaa aagaggtatt gcaaaagtta
     6661 actatgcaac tgaattaaga attgcatata gtaaaggggt taaaaaagtt ttagatgaag
     6721 attctgaggt tatagaccct aaaaagtatg gattagcagg gcttcaatca gtgaaggatt
     6781 ttgtaaaaga aaagattaaa gtgtgtgggt ctgtaaatag agtataaaaa taaaatatcc
     6841 taaatttatt ttagataagt ttaggatatt tgttatatga aaaatttata ttaattatat
     6901 gtaaatggtg tatttttttc atttaattga tgaaaaatat cagcttttat tggtaaagta
     6961 ttacatatat aattaaaata taataaaaat tgaactatat attttattat tataaaatat
     7021 gtatattaaa ttttggaatc aaaatatact aggatataaa attcttaaat aaaaagaaaa
     7081 ggttgttttt ataaaaaata ttttttatac cactttaaaa aacacaataa taacactgag
     7141 ggaggtgggg atatgagaga acgcattatt cttgaaacat taaaaaatag tagtggttat
     7201 ctaaacatag attattttgc agataagttg ggggtatcta ctcgaactat cagaaatgaa
     7261 ataaaaaaga tagaaactat aaaagaatgt aatggtttta aattagaata taagtctaaa
     7321 ttaggatata ttctaaatat taaagaccaa gataaattcg agcattatct aagaaactta
     7381 ccctcagatt tagttgaaaa tccagaacaa cggttggagt ctataatagt agagctttta
     7441 gtaaatgaag gttataaaac tatagaacag ttaagtaaaa aatttctagt aagttcatcc
     7501 caaataaaaa atgatttaaa aaagatagat gaaaagataa aggatacaga attaaaacta
     7561 gagcgaaagg ctcattatgg aattaaaata gaaggttcta ttaaagaaat tcaaagtata
     7621 ttggtggata gttattttag agggaataga aatataacag agtacaggaa taagtttata
     7681 gataatttaa aattagctaa tattagaagt acaataaaaa atgtacttaa tgaacatgat
     7741 ttagaagcaa atttaacaga acttgaggaa atattagcac aaattattat attgtatata
     7801 agagttagta tgagagtagt gggaaatgct agcaatttaa aattatatac agaagattta
     7861 attatagata aattattaga taaaattttt agagataaaa aatataacct taattatgat
     7921 gaaaagtatt atttaaagga gcttataaaa ctaaagacta aagataagaa agccactata
     7981 aagaatattg ataagaataa acttcaagat attatgttcg aattttttag agagatagat
     8041 aaaaaatata actctaattt tttagaagat aaagagtttt ttaacttatt ttacttacat
     8101 attgcatgtt taatagaacg tataaagaag aaccacaaga taataaatcc tttttcagtt
     8161 aaaataagtc aacagtatcc aactgttttt aatttatcta ttcaattttc aaaaatcata
     8221 gaaaatgagt atcatataaa aataagtcaa gatgaaatag ggtttatagc tactcacatt
     8281 gctgttcctt ttgaaaagag agaagaagct aattttaata aaaaatataa aatagcaata
     8341 atatgctctt ctggtggagg tagtgcattt ttgataaatt taagacttaa agaaattttt
     8401 ccaaatgctg agataagaaa tttttcatta ctagaagaaa aagctgtaat agaatttgca
     8461 ccagatttga ttttttcaat aacaaatctt ttatttgaaa taaatgctcc agttatactt
     8521 ataaatgaaa ttttagacga attggattat ttagatatta aagaaagtgt tagatttgca
     8581 gaccatatag gaaatatatc aagcccaaaa caatatatat taggtctatt tgataagaat
     8641 cactttaggt gtataaaaga taaagctaag tataaggata tccttgatag tatgtcaaaa
     8701 tgtatagttg acgagggagc ttgttctcca acctatccca aagatgtatg ggaaagagaa
     8761 agctatttga gtactattta tacaaatggg gtggctatac ctcatccaat cgagatgaca
     8821 ggaaacaaaa atataatatc agtagctttg atacaaactg atattattta tgaaaatagg
     8881 gtgcccaaaa ttattttcat gatttcatta ataaagggaa atttagaact tcataagcaa
     8941 atatctaaat atttaagtaa gattatggca aataaggata tggtagacat gctaaataag
     9001 tcacaatctt atgaagagtt tatgtataag ttaaaaatct atctgggagg ttagacatga
     9061 atatagagtt ttttaagagg ttgtcagaag cagatggtat agcatctaat gaagaagagg
     9121 ttagacatgt tttattggaa gaactaaagg cttatagtga taaaattatc tgtgatgggc
     9181 tagggagtat catcttttct aaaataaaag atgagagtgc accaaacgta atgatttgtg
     9241 ctcatatgga tgaagttgga tttatggtga gaagtataga caaacttggt atgattcact
     9301 taataacaat aggaggagta aaacctcttg cacagtttgt acaaaaagtt agaattacaa
     9361 caaaagaagg aaagaaaatc cctgctgtca taaatgctac atataataat ggtaaggctg
     9421 aaaatatata tgcagatatt ggagcatata cagaagaaga tgtatataac ctagggatta
     9481 atgttgggga tatggtaaca tatacaacaa gttttgagga atttacttta ccagaccgat
     9541 tagtgggaaa ggcttttgat gataggatag gatgttttgt gatgggagaa gttttaaaag
     9601 aacttaggaa agaaaatcta aattgtaata ttcactttgc ggctacaagt agtgaagagg
     9661 ttggaataag gggagcaaag acatcaacac agttaataaa tccagatata gtatttgtaa
     9721 tagatgtagc atgtgctaaa aatgagtttg tgagagacta tacaaatcag aaacaaattg
     9781 ataaaggtgt aatgctaatg catagagata ggactctagt ccctagtaga aaaatgatag
     9841 attatgtaat ggaaattgct gataaaaata atataccact tcaacatgat atgtttgaat
     9901 caggtgggac tgatggagga gaagcacatc tcgtaaatga agggaaacct tgtgtagtaa
     9961 cttgtgtacc agttagatat ggtcattgtg cattttcaat agtaagtaat aaagatttag
    10021 agaacattat aagattgtat acacagttaa ttttaaattt tgatgaagaa caatataaac
    10081 atataagaaa ttttatctaa aggagaataa gaaaatgtca acagaagtac aaataatgga
    10141 gcttatatca actgcaggag agagtaaatc gaaagctttt gaggctttaa aaaaagtaaa
    10201 gtcaaaagat tttaaaggag caaaattgtt aatagaggag gctcgtaaaa tagatattga
    10261 ggctcacaga atacagacag aattaataac tcaagattta aatgaagatg ttgaaagccc
    10321 aacaatgagt cttttaatgg tacatgctca agaccactat atgacttctc agctagcaaa
    10381 agacttgata gaggctttga tatattgtct cgataagtaa ttgtaatgta gaaatagatt
    10441 atttatgtaa aaattctttt aaaagtttag atgcttttaa gagataataa aaatacaaac
    10501 tttaggagga attattatga aaatattatt atgttgtgca ggtggatttt ctactaatat
    10561 gttaatgcaa aatatgaaaa aggtggtaaa agagagtgct aaactcaatg aaaaagattt
    10621 tgagtttaca gctataccag cagattcaat agaaagtgaa gttgatgatt gggatatagt
    10681 acttataggt cctcaaatag ctcataaaat agacttctta aaaggaatat tagagccaaa
    10741 aaataagcct tttgcagtaa tagataaaga tgtatatgga aaaatggatg gagctacagt
    10801 tatgaagttg gcattaataa cttataagaa acatcaatta gctcaaggat aataaaaata
    10861 atgagggaaa agggagatta gatatgtttt caaaatttga agcatttatg aatagagtat
    10921 ttatgccttt agctcataag gttgacaatc aacgtcacct tggttctata aaggctggta
    10981 tggttgctat gacaccattc acaatattag gaagtatatt tgcaatactt ccagcacttc
    11041 caaatatgct tggagaaaat aatccaataa gcaaattcat acttaacaat tcagcaatat
    11101 tggatttacc agtaagtctt tccataggtt taataggatt atatgcttgt atgtgtattg
    11161 cttttaatct ggggaactac tataaattat atataccagg ttgtgttgca ctatcaactt
    11221 tttctttttt attcctagta gcctcattta caccagatgg agctttagaa ataggaaatt
    11281 taggagctaa agggctattt acaggtatga tagcagggct tggttctgtg gagttatata
    11341 atttttgtaa taagaagaat atcacaataa aaatgccaga aaatgtacct gattttgtat
    11401 ctaggtcttt tgaattaata cctactacta taatagtttg tggaactttt atagttgctc
    11461 gttttatttc attaaaagta tttggagaat tacctccaca gatacttact caatttttat
    11521 ctccattagt aggaagtatg gataacccat ggtcagttct tgcacttaac tttgctatat
    11581 gtactatatt cttttttgga atacattcat cagtatttag tcctataact agacctataa
    11641 tgattacatt tatagctgaa aacatagcag ctatgcaagc tggagaaaca atacctcatt
    11701 tctttacagc aggagcttca agtgcattct ttggatttac aggatgtgga attagtatag
    11761 gttgtgtaat tgcatgtatg ttatcaaaaa atgcttccta taagaagatt ggacgtgtat
    11821 cattattccc tgcattattt gggataaatg aaccaatatt atttggagca ccgataatat
    11881 taaatccaat aatgtttata ccacatgtat ttggtggagc tataataggt acactaccaa
    11941 tgttttttat gcactgggga tggttagcaa agccagtatt tgaccctcca tatgtagggg
    12001 tattcttaga aggattttta actaatggag attggcattc tatattagca aatgcattgc
    12061 aattagtatt agcagtttta ttatattggc cattctttaa aataatggaa cgtcaacaaa
    12121 ataataaaaa acaaaaagaa gaaactaaga atatattctc taaagaagaa gaagacttat
    12181 tggatgattt aggattggat ttctaatatt ttgttatgaa taatacaata gagagattag
    12241 tatccttaat ctataaaaga ggtgttgacg ctcttttgat taaagcaaaa aatactaaaa
    12301 gatatatagg agctttatca ggtagtggtg tatatgtgct tgttactaag gataaaaatt
    12361 atcaaattct agatggcaga tatattgatg aagcagataa aaaaacgact ggtttcataa
    12421 aaaggattgt atctcaaggt tcttatatac caactataat tgagttatta aaagaattaa
    12481 atataaaaaa gttagcaata gaaaaccaag ctatgagtat acaagagtat ctttcactaa
    12541 aaaatgaagg ttttgaaatc aatttaataa caaatgaatt atggaagcta agggctataa
    12601 aatctaaaga agaaattgaa cttattaaaa aggcttgtga gattacagat gaagtatttg
    12661 atgaggttat ttctgaaatt aaaaaagata tgactgagct agaagtatca gctctaattc
    12721 agtatcatgc attaaaaaaa ggtgcaagtg gaatgtcttt tgaaacaata gtggtatcag
    12781 gagaaagagg tgcaatgcca catggaagac ctacaaacaa aaaattgaaa gtgaatgaag
    12841 ctataacaat agattttggt gttgtttatc aagggtatca atcagacatg actcgtacaa
    12901 tatctatagg taaacctcct aaaattatta aagagatata tgatgtagta ttggaagcac
    12961 aattatctgc aatagagtca ataaaggaag gaactagggc aagtgatgtt gacaaagtgg
    13021 ctagaaaaat tatagataaa catggatttg gcgaatattt taatcatgga ctgggtcatg
    13081 gtataggtct tggagatggt gaagttccca ctttaaatcc aaatagtgaa gatatattag
    13141 tggaaggaat ggtaatgtct tgtgagccag gtatttatat tccaaatgta gggggagtta
    13201 gaatagaaga tgatattgtt ataatagatg gaaaaggtgt tccactcaat aagacatcca
    13261 aagagtttat aatattagga gagtaaaatt atgaaatata attttaataa agtattaaat
    13321 cgtaaagaag gaaattgtag aaaatggagc aaccatgtga ttaaagaaaa attcggctta
    13381 aatgaagatg caattcctat ggatttagct gatatcgatt ttgagtgtgc tcctgctata
    13441 aaagaatcta tcttaaaaag agcatcagta ggagattata gttatacttt tataggagat
    13501 gacttctatg atgctattat aaattggaat aaaagaagat ttgatgtata tatagaaaag
    13561 gattggataa aattaacttt tggtactgtt tcaacccttc attatatagt tcaagcatat
    13621 actaaagaag gagagggtgt attgataaat acacctgctt atgacccttt tgcagaggct
    13681 gtagcaaata ataatagaaa gttgtgttgt agctcattaa aattagataa caatagatat
    13741 tatttagatt tagaggatat ggaaaatcaa atgaaatacg agaatataag attacttata
    13801 ttctgtagtc cacaaaatcc atcaggtcgt atatggacaa aagaagaact atatcaagtg
    13861 tcagagttat gtttaaagta taatgtaatt ttggtaagtg atgaaattca tagagatata
    13921 atatttaaag attatagatt tgtatctttg tggaatgctc atccagaaat ttataaaaat
    13981 agtataatct gcgtatctcc taataaaggc ttcaacttag gtggattaaa aacatcatat
    14041 gtattaataa gagatgtaaa aattagggag actttattag aaaggctaaa aagtaattca
    14101 ataacttctc caaatgtatt tgcaatacca gcaatagttg cagcatacaa tgaaagcgaa
    14161 gaatggctag atgctatgac tagttatgta gaggagaatt ttgaaatagt ttatgatttc
    14221 tttgaaacta atataccaaa agccaaagtg atgaagtcag actcttcttt tttagcctgg
    14281 atagatgtaa gagaagtatt tagaaatgaa gaagaatcta aagaattttt tagacatgca
    14341 aacttaacta tggtagttgg aagttatttt gtgaaagatg gagatggatt tattcgtatt
    14401 aatgtggggt gtcctagaga aacattaaat gaggctctaa atagaatcaa aaaaacgtat
    14461 ataagtatgt attgttaaat cataaaaaaa gtatctatgg tttatataaa aacacattat
    14521 ttagttatca aatattactg gaattttgta ttccagtaat attttttgat taaggaatat
    14581 tattgaggaa tattaaataa tattatttca tacccttata ttaacttata taaaaatatt
    14641 ttattatttt gggaacgggt tagctaacct ttatctagtg tatatttata ttgtaagaag
    14701 cagtaaaaac ttttccctta ctaagtttat taataaactt cttatcttta taaattttgt
    14761 tagaatttat actatcagca agaccatacc aaggttctat acataaaaaa ggagcagtag
    14821 aattagtttc actataataa ggagtccaaa taccaactag agggaaatct tccatagata
    14881 tagttacata tttactacca tcattattat atatagacac ttcatctatg ttggtgtata
    14941 tcaaagcatc attcttgaat acttctggat ttaaatgtaa gttttttaat tttccaatag
    15001 gtattatatc atctgtaaaa gaaccattta aattgatttt ttctacatca tttttacttt
    15061 taaactcaag gtagtgttta gaaaaatcat tttgtttgta aaaaggaagg ttgaatgcag
    15121 gatgtccacc tatagaaaag aatatatctt tagagtcagt attttttaca atccattcta
    15181 cctcaatact attatcattt aatgtatagt ttatgattag ctcaaaagaa tatggatact
    15241 tctttaaagt ggattcatta tctgttaatt tataagatac aaatgtatct cctttatcta
    15301 caatctcaaa gtccatatct ctagcaaacc catgttgagt catgctatat aaattttctt
    15361 ctattattgt ttcattatca aaaagtttac ctactatagg gaacaatata ggtgattgtc
    15421 taccccaata ttttttatta ccatcccaaa gaatgttttt gtcgtatttt ttagaaaata
    15481 ttttagttag ttcagcacca aaactgcttg attcgataat taaattttca ttttgtaata
    15541 tattcattta aaatctcctt tattatattt tatgagtata ttatactata aagtataaag
    15601 attattaaat tttatttgat attatatttg aaaggtagat tttagtatat tttgaaaatt
    15661 ttgttttgaa aatttgggat tatttaagaa attttgtatt ataaatagaa ggaaatagat
    15721 aaattgtata gaataaaata tattaagata agttaataat gttaaatggg aagtgagttt
    15781 atggaaaaac caaatacaaa agttgttaac tttcaagagt ataaagacag aaaaaaccaa
    15841 agagaagtaa atagagacga acttttaaag cttataaaaa aaattgtgac ttcaaaatag
    15901 ggagttgtta aactccctat tttaatgggt ttcataataa ttaagacatt ctgagtccat
    15961 ctatataatc tttgaattgt gagaaatctg attcactact taattctatg gtttctgaac
    16021 cttccgtata aaaattctta agatagatag tataattatt atcaacacaa tcatgataaa
    16081 tacatctata tccatcttca tacaaacttt ttaacttaga aaaatcatcc acaatatcat
    16141 ctccttaaag ttcttttaaa tatattttta caaaaagcat ctaattgtat gcaaattgac
    16201 aatgtaaaat aaaggaaatg aacataaaat gtagaataat ttttatatgt atgtaaaaat
    16261 aaaaagaaaa agttgaaatt tttagaaaat aagagtattt tttatctaaa aagagagttt
    16321 aaaaaataaa aaaatgttgg gaggataaaa aatgagttta ttagaaagta tatcaaaaaa
    16381 catatattcc ctagataact cttctattga aaaaacaaag cagagattgg ataggcttat
    16441 acatccaact ggaagcttag gaaagataga agatatttgc atgcaattgg caggaatatt
    16501 tggcaatgaa aattttgaca caagtaaaaa ggttataatt gcttttgcgg gagaccatgg
    16561 tgtatatgaa gaaggcgtag cacctgaccc tcaaaatata acaaaattac agtttccaaa
    16621 tttttcgaaa ggtctatgtg gagttggagt aataagtaaa tttgtagggg cagatgttgt
    16681 agctgtagat gtaggaataa actgtgatga aaaactggat ggggtattag actacaagat
    16741 aagaaaaggg acatcaaata tggcaaaagg tccagctatg agtaaacaag aagccattag
    16801 atgtttagag ataggtattg aaatagcaga gcagtgtata gaaaaagatt ataaggttat
    16861 tggtattggg gagatgggta tagcaaatac tactccaagt acagcaataa tatctgtaat
    16921 ttcagggtgt gacccgttag aagttactgg tataggtgca ggtcttaaga aagaaagact
    16981 aaagcataag gctgaagtaa ttagaaaggc tattgaaatt aataatccaa atccaactga
    17041 tggagtagat attttatcta aagtaggagg atttgaaata ggctcaatgg caggtgttat
    17101 attaggttgt agtgctaata ggattccagt agtaattgat gggtttattt cttatgcagc
    17161 agcattaatt gcatataaga taaatccaaa aactagagaa tatatgatag catctcattt
    17221 atcagcagaa tcaggaacta aaagagcatt agacatatta aaattagacc ctctattaaa
    17281 tatggatatg agattaggtg agggaagtgg agctgcttta gcatttgata taatagaagc
    17341 ttcaaactat acttacaaaa atatggcaac ttttgatgaa attgacatgg gaagataaat
    17401 ataaaaattt aatatgtagg agtgttttga tgagtaaaat tattctagta acaggaggag
    17461 ctaggtcagg gaagagtaac tttgcagaaa aactatgttt tgacagaaat aataatacag
    17521 cttatatagc aacctctatt ccatttgatg atgaaatgaa agatagagta aaaaaacatc
    17581 aagagaatag acctagaaac tggaatactt atgaaatata tgaagatata tattccataa
    17641 ttaaggagat atataaagaa catgaaactg ttatactaga ttgtgtaaca ttattagtaa
    17701 ataatctaat gtttgaacac aatctaaata tagaagagtc tagtcaagaa gaaataaaca
    17761 aattagaaca atacataaaa gaacaagttg tcaaacttat tgaagaaata gaaaaaacaa
    17821 atctatactt tgtatttgta acgaatgagt taggtatggg aattgttcct gggaataaac
    17881 ttagtagaat ttatgctgat attgtaggtc gtataaatca atatatagct tcgaaaagta
    17941 atgaagttta ttttgtagtt agtggcatag ctatgaagat aaaggagtag tatgcaatta
    18001 tgaaaagatt tattttaata ctacagtttt tgacaaggat acctataaaa ttaaatgtag
    18061 gatttgatga tgaattttat aagtcaatag tatattttcc acttgtaggt tttgtcatag
    18121 gtatattatc ctatttaata ggatggattt ctatgctttt atttgaacca tttatagctt
    18181 ctataataat aactctagct ggtgtcttaa ttactggagg acttcatata gatggattag
    18241 gagatacatt tgatgcaata tatagttata gggataaaga aaaaatgctt gaaataatga
    18301 aagattcaag attaggtaca aattcacttc tagcaattat gtttgtactt ttattaaagg
    18361 ttggattcgt ttacgatata ataagtaata attcattgtg gattataatt tttatgccaa
    18421 tgattgcaag gttaggagta atgttattga catataaaac cgtgacacca agagaaaaag
    18481 gaatgggaaa tttatttata ggaaaattaa ctacaagtat gttaataact gctataatat
    18541 acacattatt gatagttgct cttattacta aatttatatt tttattacct aatatagtgt
    18601 tgattaaagt attaggttcg attatagtgg tttttgtatt tataatatta ttcaaaaaac
    18661 atatttataa aaaaattgac ggagtaacag gagatatatt aggctgtgga atagaacttt
    18721 cagagcttgt atatttaatt tacatatatt tgttaatttt tatgtttttt taaagtaaaa
    18781 agtggtgata atatgataag attaatatta ataagacatg cactgacaaa tgataataaa
    18841 aaaggaagat tatcaggcca tataaatagt tgtattagtg aagagggcaa actacaaata
    18901 aataaaatta ccagatatct gagtaatgaa aacatagata aggtttatac aacaccatct
    18961 acacgtacta aagatacagt agaaaaaata tcaaaattga aattattaga aatagaagaa
    19021 aaagaagctt taagagaaat tagttttgga gactttgaag gaagaacttt tgaagaaatt
    19081 aaaatcaaat atccaaatga gtttgaaaaa atgataaaag aaggtaacaa ctatagatat
    19141 ccaaatggag aaagtttgat agactcttat aaaagagtgg caaaagaaat tgataatata
    19201 attttagaaa atgatagtaa tttagataca aagacaatac ttatatgttc tcatgcagga
    19261 acaataagaa atataataac acatttgatt tctggtagtt ataagtatca ctggaacttt
    19321 aaaattgata atgcatctat tacagtttta gaaattgatg gtggatttgc tgtaatagat
    19381 aaaatgaatt ttacagactt catataattt aatcaaaacc aaacattaca atctgtgttt
    19441 gtaatatatc tgtaaaatta gcaatgaaaa tagaatatta gattaaaatt aggttcttaa
    19501 ttagatgtta aaagggaaaa aggttagatg cctttgcagc ccccgctact gtgaaaccaa
    19561 cgaaactata aataccactg tcagtttgat gggaaggtta tagaagtagg atgaagttaa
    19621 gccaggatac ctgcctaatt taatttaaaa caagaggtct tcggggtgaa agatttttaa
    19681 ttaatatata taaaagtcct atctttagaa gtattaggac tttttttaat ttgtttttta
    19741 gtaaatttta acaaggaggg atggaaagtg gggaaaaaaa taatgctaca aggaacagct
    19801 tcaaatgtag gtaaaagtgt cttagtagca ggtttgtgta gaatatttaa gcaagatggt
    19861 tatactgttg ctccttttaa gtcacaaaat atggctttaa attcttttat aactaaagaa
    19921 ggattagaaa tgggaagagc acaagtattt caagcagagg ctgcgaaaat agagccaata
    19981 gctgatatga atccagtttt attaaaacct tctgggaata ataaaagtca agttattgta
    20041 agaggtaaag ttgtaggaga aatgccttct tctaagtatc atgattataa attggagtta
    20101 gtaggtgtgt tgaaggaaac atttaatagc ttaaattcaa ggtatgatat tgtagttatg
    20161 gaaggggcag gaagtaatgc agagataaac ttaaaagata gagatatctc taatatgggt
    20221 atggcagaaa tagctgatgc acctgtaata atagtcggag atattgatag aggaggcgta
    20281 tttgcctcat tagcaggaac aatgctttta ttaaatgaag atgagaaaag aagagtaaaa
    20341 ggtgtaataa tcaataaatt cagaggaaaa aaagagcttt tacaagatgg tgtaaagatg
    20401 ctagaagaca tcataaaagt acctgttata ggagtagtac cttatacaga tataaaaata
    20461 gaagatgaag acagtgtaac tacgagattt aagaaaaaaa tgaataaaag tgatatacac
    20521 atagaaataa ttagaacccc acatatgtca aattttacag attttaatat atttgagaca
    20581 caagaagatg taagtattag atatgtagac tatggagaat ctttaggaaa tccagatata
    20641 ttaataatac ctggaactaa aagtactata gacgatttaa catatataag aaaaagtggt
    20701 cttgaatcgc aaatcaaaaa tttacatagt caaggtaaat tgatatttgg gatatgtggt
    20761 ggataccaaa tgttaggtaa aaaattaaaa gacccttatc atgttgaaag tgaaattgaa
    20821 gaagttgatg gaataggtct tttagataca gaaacaacgt ttgagaaaga aaagactaca
    20881 acacaagtag aggctactat tgttgaatgt atcagtgaat acatgaataa ccttaaggga
    20941 aaaaaagtaa aaggttatga aattcatatg ggcattacaa agcgaggtaa tgatgtaaga
    21001 agtttagatt taataactaa aaaacttgat aaagaagtga attatactga aggaagtgta
    21061 aataaagagg ggaatgtaat tgggacttat cttcatggaa tatttgatga aataagcttt
    21121 acaagagcaa ttttaaacaa tataagaaag aaaaaaggtt taggagaatt agaaagcaaa
    21181 gtaaactctt ttaatgaatt taaggataaa gagtatgata agttggctga ttttttaaga
    21241 gaacatttag atatagagaa aatatatgag attatgaaat aatgatgacc tttatttata
    21301 atttaggtag gaggtgtatt atgaaaaaaa ttttaattgc tggtactaat agtggagtag
    21361 gtaaaacaac aatatcatta ggaataatgc aagctttggt taagagaaat atgaaagtac
    21421 agccatataa agtagggcca gactatatag acccttcata tcacactttt ataactggaa
    21481 gacattctag aaatttagat tcttatatgt tagatgatga aaaaataaag tatatagtaa
    21541 ataagtcctc aaaagatgca gatatttcag taatagaagg tgttatgggc ttatatgatg
    21601 gttttggaat agatttagat aattgtacaa gttcacatac ttcaaaggta ttaaagtcac
    21661 ctgttatatt agttataaat ggtaaatcta tggcagcatc tagtgctgct atggtacttg
    21721 gatataagga attagataaa gatgtaaata taaagggtgt tatagtaaat aatgtaaaga
    21781 ctaatagcca ttatcaaata attaagagtt ctatagaaaa atattgtaat gttgaagtat
    21841 tggggtattt tcctccaaat aacaaattct ctttagaatc tcgacatcta ggattagttc
    21901 ctagtgtaga aatgaaatca ctaagggaaa aattttatac cttagcagat gaaattgaac
    21961 actatataga tattgacaga ataattgaaa tatcagaaag cgatgaattt gagagtgatt
    22021 ttgattttca aactttgata gaaaataata aaataaaaga acatgttaat aataaatcaa
    22081 tagctatagc atatgataaa gcattcaatt tttattatag agaaaatata gagcttttag
    22141 aggaacttgg tttagaaata aactatttta gccctcttga agatgcatca gtgccaaatg
    22201 cagattatat ttatattgga ggaggttttc cagaaatatt tggaaaagaa ttagaagcaa
    22261 atgagtcaat gagaacttcc atattagaag cacataacaa taatatacct atttatgcag
    22321 aatgtggagg actgatgtac ctaggtgaga aactattgaa tcaagaagaa caagaattta
    22381 atatggttgg aatatttagt ggaattagta agatgacatc ttctttaaag agatttgggt
    22441 actgtattgg tgtagccaag gaaaatactc ttttatctaa aaaaggggaa tctattaaag
    22501 ggcatgagtt tcatcattca atatttgaaa gtgatgaaaa atgtgtatat tcaatgagaa
    22561 aagaaagaga tggaaatata gtagaagagt gggatggtgg ttatagtaaa ggaaacactt
    22621 tggctacata tcttcatact catttttata ataacttaaa ttgtatagaa aattttttga
    22681 aaaaaggaaa tgaataaatg agtattttat ctatttatat aggatatgtg acagacttaa
    22741 ttgtaggaga cccatattct tttcctcatc cagtgagatt tattggtaaa cttataaatt
    22801 ttactcaagg tataataaga aaggtctcta aaaatgatag gcagttaaaa tttgggggat
    22861 ttatattatg gtttataacc gttggaataa catatttaac aacttatgct atagtgaagc
    22921 tatttagttt taatatatta ttgagtattg tagtgaatag ttttataatt tatacaacac
    22981 tagccacaaa atgtttaaaa gacgaagcat taaaaattta taatgtatta aaaaccgaag
    23041 atatagaaaa atctaggatt cagctatctt atatagtagg tagggatact actaatttaa
    23101 gtgaacctga aataataaga gcaactgtag aaactgttgc agaaaatact gtagatggta
    23161 taattgcgcc tatgttttat gcatttattg gaggagcacc acttgctatg gcctataaag
    23221 ctataaatac attagactca acagttggat ataaaaataa aaagtataaa gatattggtt
    23281 ttgcatctgc taagattgat gatatagcca attatatacc agctagaata tctgttattc
    23341 ttatgacaat tggaagtttc tttttaaatt ataactatag gaactgtttt aaaatctcta
    23401 ttagagatag aaagaatcat aaaagtccaa attgtgcttt ttcagaaggc gcagtatctg
    23461 gtgctctagg aatacaacta ggaggtacca acgtttattt tggggaaaaa gtatataagc
    23521 caacaatagg ggataagctt agggagatag ataaagaaga tataattaaa actaataaaa
    23581 ttatgtatgt atcttcattt gtatctatgt tggtatttac actaatacat gtgttatgta
    23641 ggatgatttt aattaactta attatataaa ttattgtttg ctaacttaag ggggacatag
    23701 atgaaggatt tagggcatgg tgccaatgta ggtgaaatgg caagattata tgggaaaaac
    23761 ccaaaagaaa taattgattt tagttcaaac ataaatccaa atgtattacc aaatttagag
    23821 agatacattt taaaaggatt agaagagtgt aggaattatc cagatataaa ttatacaaac
    23881 ttgagagaaa atatatcaaa atatataggt ataaatccag actttataat acctggaaat
    23941 ggtgcaacag aagtgattta tttgcttatg aagagtgtta aaaaaaaatt ggctataata
    24001 aatccgactt tttcagagta tagaagaagt gctgagttaa ataatttaga tattatagat
    24061 ttagagctag atttagaaaa taactttaaa cttaatatag atataataaa agaaaatata
    24121 aaaagatttg atagtttatt tatatgtaat cctaataatc ctagtggtaa tgtccaagat
    24181 ttaaaggaac ttgttcattt actaggtaaa cataataagg tactaataat tgatgaaact
    24241 ttcatggaat ttgttgaaga tgaaagtaaa tacagtttgg ttaaatatat agaatcaaat
    24301 aaaaatatat ttataattaa agctgtgact aagttttttg gtatgcctgg tttaaggcta
    24361 ggatatggtc ttacaagcaa cactgaaatt atgaacaaaa tttatgaaca caaagagcca
    24421 tggactatta attcttttgc agatatacta tccaatttta tttttgaaga taaagagtat
    24481 attaaaaata gtaaagaata ttacatagaa gaaagaaaat atatgttaca agagttgaga
    24541 aacataagaa atataaaggt ctatgataca gatgctaatt ttattttgat aagagcatat
    24601 aaaaaaacaa ctaaagaatt gaaaaaagat ttgcttaagc aaggaaacat attagttaga
    24661 gatgcgtcta attttatagg attagatgat agttttataa gagtggctat aaaatctcat
    24721 gaagataaca aaattcttat agaaaatatg aaaaatttat tgggtgatta aaatgaaatc
    24781 ttatggaata tgcccagcat cttgtggaga atttgtacaa ggtataattg atgatgaaga
    24841 atatttatgt tcatatgcaa tagatatgta ttctgaagtg tatattgaag aaaaattagt
    24901 agatataaat ttagggagat ataagtcaag gttagcaatt gaaaaggtat ttgaaaaatt
    24961 taatttacct aaaaaatata ctaaaaatat ttctttaaat ataaatagta aaatacctgt
    25021 tggaaaagga atggcaagtt caactgctga tataggagct acaataaagg caacattatc
    25081 attgatagat aaagatttaa gtagtgagga gatttctaaa ttagcagcag aaatagaacc
    25141 aacagattct atatttatag ataaaaatag tatatttaat cctctaaatg gaactgttat
    25201 aaaataccta ggaaatctga ccaatgcgaa agttataata cttgaaccaa acaaagtact
    25261 agatactatg aaaattagat tgaggcaaga ttataataaa ttaaaagtag aaaataaaga
    25321 agtaataaaa aaatcatttg ctcttttaga agaaggattg aaaaaaaata atttatctct
    25381 agttggagag gcttgtactt tgagtagctt agcaaatgag aatatagaaa aaaaagagta
    25441 tttaaatgaa ataataaaaa tatcaaaaaa atatggagca tatggagtaa atattgctca
    25501 cagtggaact gtagtaggta ttttaattga taagtctatg aatgataaaa agatgataga
    25561 taccttatgt gaatctaaca taaattctgt ctataataaa atttatacac aaaacataat
    25621 taatggagga attaaggggg aaatagaatg gaatacataa agaatcctat gaaaatagaa
    25681 gaaaaaagtt ttgaattgat acaagaaata atagacgaaa ttagaccaga ttataaattt
    25741 aagaataata ttgaagaaaa aataataaaa agagctatac atactactgc tgattttgat
    25801 tacttagata tattaaaaat atcagaagaa gcagttgata gtataataga tgcattaaaa
    25861 aataatgcaa gtatttatac tgatacaaat atggctctta gtggtataaa taaaagaaag
    25921 ttagaatcat taggatgtaa gtataaatgt ttagtggcag atgatgaaac tgtaaaatta
    25981 gctaaagaaa aaggaattac tcgttctatg gcagcagtag aaattgcagc taaagaagaa
    26041 ggaagaaaaa tattcgtttt aggtaatgct ccaacagctt tatataaagt tatggagatg
    26101 aaatcagaag gtagattaga tttagatgca gttataggag ttcctgtagg ttttgtagga
    26161 gcacaagaat caaaagatga agttgaaaaa acagatatac catatataat ttctaaaggt
    26221 agaaaaggtg gcagtaattt agcagcagca atagtaaatg ctatattata tactatgtag
    26281 gtacctatat ggaagaatat gtatatatag atggaaaaaa atatagaaga ggatatacta
    26341 caggttcatg tgcaacaggt gcatctaagg ctgctgtata tatgttgatt acaaaaaata
    26401 gaatcaatac tataaatata gatactccta aggggatacc attactacta aaagttgata
    26461 atataaatat ttctgataca tttgtggaat gttccataaa aaaagatggt ggagatgata
    26521 tagatgcaac tcataccatg gatatttatg caagagctga aattgtagct aaaaatgata
    26581 agaacaaagg gtatcttact ctaaaggata tagatagttt aagcaccaat agtgagtgta
    26641 aaagtgagct ctataagttt attagggttt atggcggaac tggtattggt gtggttacga
    26701 aaaaagggct tagtgtggat gttggtaaac ctgctataaa tccgactcca cttaaaatga
    26761 taaaccatga aataagaaag cttataggag ataattttga atctatactt ggaaatgata
    26821 aagttttaaa aataacaatc tttgctccac aaggagaaac tgtagcaaaa aaaacattta
    26881 atccaagatt aggtatagtt ggaggaatat ctataatagg tacaactggg attgttgaac
    26941 ctatgagtga tgagggttgg aagaagtctc tttctataga acttcagatg aaaaaagaac
    27001 aaggcttaga taaaataata ttggttccag gaaatcatgg agaacagttt ataagagaaa
    27061 aattaaactt agatattaag tatgtagtta gggtaagtaa ttttataggc tatatgatta
    27121 aagaagctca aagaatagga tataaaaaaa tcttaatggc aggtcatata ggtaaattta
    27181 taaaagtatc tgctggtatt tttaatactc atagtaaggt agcagatgct agaagtgaga
    27241 tattagtggc aaacttagct ttaatgggag ctagatacga atttttaaat aaaataaatc
    27301 aatgcgtaac tactgaagaa gctgtagagc taataaataa tagtgaatat agagaagtat
    27361 ataatatatt aagtaataaa tgtaaggaga gagtaaagca atacttgaat gaagattcag
    27421 atgatattga tgtggaagta atcatatttt caatggataa gtctttatta ggtaaatcgg
    27481 ataatacaga tgatttagtg gaggtcttta tatgataaac ataataggtc taggacctgg
    27541 taacttagac tatataacaa aaaaaggtga aaatttaatt tccacttctg atgtattaat
    27601 tggtggtaaa agaaatctag agtctataaa aaattttgaa ggagaaaaaa tagttctgga
    27661 ttctaattta agagaaatta tagagtacat aaataacaat aaagaaaagc aaatatcaat
    27721 aatagcatct ggggacccat taatatatgg gataggtaga tatctatcta aaaatattga
    27781 taataagatg ttaaacgtgg tttctgggat tagttcatta cagtacatat tttcaaaaat
    27841 atatgtagat atgaatgatg tatatattac aagtagtcat ggcaaaatac cagattttga
    27901 ttacatatta tcacataaaa aagtatgtat ggttacagat tcaaagatag gaccaaaaca
    27961 aataagtaga gaaataatag atagaaatct aaataaaatt attgttgtag gagaaaatct
    28021 gtcatatgac aatgaaaaaa taacaatagc gaaaccagaa gagataataa gaatggacaa
    28081 ttttgatatg aatgttgtgg tgattttaga tgaggaatag tgaatttata acaggtaaag
    28141 ttccaattac taaggaagag gttagagcaa tatcaataag taagttaaat ttagtaaatg
    28201 ctaaaaacct catagacata ggggctggta caggaagtgt aagtgttgaa gctgcatata
    28261 attatcctaa tttaaaggtt atatctatag agaaaaatga tgacgctata gagcttatag
    28321 agaaaaatat aaaaaaattc aacttaaaaa atatagaagt gataaaagga tatgctccaa
    28381 ttaatctagg gttaaatgtt aaagttgagt ctatttttct tgggggaaca ggaaataatc
    28441 tggaagaaat aatagaatgg tctaaaaaaa atctcgttac aggaggaaga cttgtagcta
    28501 attttataat aatagataca tttaatcaaa ctttaaaact gcttaaaaag catggattta
    28561 aagaaataga tgtatgtgtt ttgaatgttt ctaaactaga gaaactagga aaaggtgaat
    28621 attttaaacc actaaatcct atatatataa tatcttgtga aaaaggggaa aatgatgatg
    28681 aataaagtac attttgtagg agcaggacct ggagataaag agctgataac attaaaagga
    28741 tacaaattat taagtaatgc agatgtagtg atatatgcag gttcacttgt taatcctgaa
    28801 cttttggaat actgtaaaga agattgccaa atacataata gtgcacatat ggatttacaa
    28861 gagataatag atgttatgag agaaggtata gaaaataata aatctgttgt aagactgcaa
    28921 acaggtgatt tttctatata tggttcaatt agagaacaag tggaagattt aaataaattg
    28981 aatatagatt atgactgtac tcctggagta agttcttttt taggggcagc atcatcttta
    29041 ggggttgaat atacagttcc agaaatttct caaagtgtaa ttataacaag aatggaaggc
    29101 agaactccgg ttccagagaa agaatcaata caatcatatg ctaagcatca gacatctatg
    29161 gtaatatttt tatctgttca agaaattgag aaagttgtgt ctaaattact ggaaggtgga
    29221 tatccaaaag atactcctat agcagttata tacaaagcaa cttgggcaga tgaaaaaata
    29281 gttaaaggaa ctttaagtga tatagctgta aaagttaaag aaaacaacat aaataagact
    29341 gctttaataa tggttgggag atttttagga gaagagtata ataattcaaa gttatatgat
    29401 aaggatttca aacatgaata tagagggtaa tattgctata atctgtatta cagaaaatgg
    29461 aaaaaattta gctgtaaaga taaattctct agtaaataac tgtgttatat atcaagtaaa
    29521 aaataaaaaa agtaatttag ttgttgaaga aacatgtgta catacagttc aaaagaagtt
    29581 aagtgatttt gtaggagaaa tatttagcaa atttgattac ataatattta taatggctac
    29641 aggtatagtt gtacgaagta tagcaccata cataataagt aagtttagtg accctgcaat
    29701 tttggtaatg gatgaaaaag gcaaaaacgt aataagttta ttgagtggtc atattggagg
    29761 ggcaaacagc ttgacacagt acataagtag tcttattgat gctaatccag taataacaac
    29821 agctactgat ataaatcaaa aagcttcctt agatatgata gcaaaaaaac taaatgcaca
    29881 tattgataat tttagagaaa gtgtaaaaga agtaaattct atgctagtga ataataaatg
    29941 tgttggatta tatgttgatg aaggatatca agttgatact aggggattta aacttataga
    30001 aagtaaggat gagttaagag atagcttttc aaacacatat atagaaaaat tagttgtagt
    30061 aagcaataaa ataaattgta ttgaagatat tgagaattta aacttagata ataaatttga
    30121 acattgctat agtactaaaa aaataataaa ggtaattcca agagatatag tgataggaat
    30181 tggctgtaga agaaacacag atagtgagct gatgtataat tcacttgaag agtttcttaa
    30241 taaacaaaat atagatatta gagcaataaa cacagttgga agtatagaac tgaaaaaaga
    30301 cgaaaaagca ataattgaat tatcaaaaat actaaaagtg ccatttaaaa cattttcaat
    30361 agatgaaata tctaaggttg ataatctttt tgataaatct gattttgtta aaaaaaatgt
    30421 gggcgtgtat tcagtagcag aacctatagc atatcttttg agcaaaggta atttgttagt
    30481 tgaaaaacat aaatataaag gaataacttt ttcagtgggg agaataaaaa tatgatatac
    30541 gttgtaggaa ttggaccagg aagtaaagat acaatgactt tagaagcaat aaaagcaata
    30601 gaagattctg aggctattgt tggatataag acttatataa aactaataga agaatttata
    30661 caaggtaaag aaataatcca aaatggtatg aggcaagaag ttgatagatg taaacaagca
    30721 gtagaggaag cgaaaaaagg aaaaaaagta gcagttgtaa gcagtggtga tgctggaata
    30781 tatgggatgg caggtctcat tttagaatta atatctaaag aagatgaaga tataaaagta
    30841 aaggtaattc caggtgttac tgcaagtatt ggtgcagcag ctatacttgg agctcctatt
    30901 atgcatgatt tctgtcatat aagtttgagt gatttaatga ctccgtggga agtaattgaa
    30961 aaaagattga gattggctgc tgaagccgat tttgtaattt gtttgtataa tccaagaagt
    31021 aagggaagaa gtgaacatct aagcaaagca tttaaaataa tgggtgagtt taaaggtgga
    31081 gatacacctg ttggagtggt aaaagatgta ggtagagaaa aagaagaaaa gtttgtatgt
    31141 acttttgaaa atatggactt tgaaaaagtc gatatgacta caatggttat aataggaaat
    31201 aagtctacct atattaatgg tgaattgatg ataactccaa gaggttatac agtatgattt
    31261 taattttagg aggaacttca gatagtttat ctatatgtga taagataaat gaactgaaaa
    31321 atcaaccata tatcctttca gttactacag attatggaag ggaacttgca caacaacatg
    31381 cagagaatgt aattttaggt aagcttggaa aagaagatat gctaaaattt ataaaagata
    31441 ataatatagt aaaaataata gatgccactc atccttatgc agttgaagta tccaaaactg
    31501 ctattacttg tgcaaaatta ttgaatatag attatataag gtttgagaga aaatctttaa
    31561 ttgaagaaat agattatgat aataaattta tagtagatac aattgaagaa gcctgtgaaa
    31621 tagcaaataa aatagggaca aatatattca taggaacagg aagtaaaaat ttgaacagat
    31681 ttatagagga aataagtgat aaaaatttag ttgttagagt tcttccaacg agtgaagtca
    31741 tattaagttg tgaaaaatta ggtttaagtg cagataatat agtagctatg aaaggtccat
    31801 ttagccagta tataaatgaa gaaacatata aacattacaa tatagattta gtcataacaa
    31861 aagaaagtgg aatagctggt gggttcttag agaaggtaaa tgcatgtaaa actttcaata
    31921 taccagtaat aattataaaa agagaatcga ttaactacga aaatgtattt aatgacataa
    31981 ataaaattaa agacatactt gtgtaatttt taggaggaga atatatgaag aaagccttat
    32041 tagtagtgag ttttggtaca agctatcatg aaacgagaga aaagacaata gatatatgtg
    32101 aagataaaat aaaaaattcg ttaaaaagcc atgatttttt tagagcttat acatcaaata
    32161 tgataataaa taaaataaaa aaaagggatg gaatagaaat agataatcca atacaggctt
    32221 tagataaaat atatgaacaa ggatatgatg aagtaataat ccaaacactt catattatat
    32281 gtggcgaaga atttaacaaa ttaaaagaac aagtagatgg atattcttct aaattcaaaa
    32341 agattgtatt aggaagacct ttgcttacac atattgaaga ttatcaggaa gctgtagagg
    32401 caattaagca tcaaattcct catatggaaa gtaatgaagc agtagtattt atgggacatg
    32461 gaacacttca tgaatcacat tcagcgtatc cagcattgga atacatgctt agggataatg
    32521 gaataaatgc ttatgttggt acagttgaag ggtatccaga aatagaacat gtaataagaa
    32581 gattaaaaga aggaaatata aaaactgtaa atttgatgcc atttatgtta gttgcaggag
    32641 accatgctat aaatgatatg gcaggagatg aagaagattc ttggaaaaca atattagaag
    32701 aagatggatt tgacgtaaga gttcatttaa aaggactagg agaaaattct tatatacaag
    32761 ataaatttgt tcgtcatgct gtaaaatgtg cagagaatgc taagttctat ggtataggag
    32821 cagggccagg agatggttct ttacttacga taaaagcggt taatacttta aagaaactgg
    32881 atattctata cactccagaa tcaaaaaagg gtggagatag tctagcttta tcaatagtta
    32941 gtgaatattt acctgaatca gtggaaataa agtctaggca tttcccaatg agttttgatg
    33001 ggaatgaaaa aagtttagct tgggacaatg tagcacaaga aattatatca gatgttaatg
    33061 atggtaaaaa tgtaggattt gttacattag gagaccctat gatttatagt acatatgtat
    33121 atataatgaa aagaatatta ggagaggtag aagtcgaaac tataccagga atttcatcat
    33181 tttccaatat agcatctaat cagaattttc cattagtcat ggataaagaa gctttagtgg
    33241 taattccttg tactatggaa gatgataaga tagagtatgc tttgcaaaat tataattcaa
    33301 tagtattgat gaaggtatat aaaaatttta aagaaataat aaaaaaatta gaaaagtatg
    33361 atttaataga acatgctatt ttagtaagta attcttccca agaaagcgaa aatgtgttta
    33421 cagatttaaa agaagcgcac cttgaagata agatatctta tttttctact atactagtaa
    33481 ataaagataa taaaataaaa taggtgaaag caatatttaa aggagactta atatgaatat
    33541 agtggttgga actaggggta gtactctagc gcttatacaa acagagtggg taataaatga
    33601 attaaaaaag aagtatccag aaattagttt tgagattaaa atcataaaga caaaaggaga
    33661 tttaattcaa aatgtttcac tggataaaat aggtgataaa ggattgtttg tcaaagaaat
    33721 agagcaacaa ttattagatg gaaaaataga catagcagta catagtatga aagatatgcc
    33781 ttcttattta gcagatggac taaaatttgc tcatacgcct aagagagaag acccaagaga
    33841 tgttctaata cttagagaag gatataaaaa tttagatgat ttgcctcatg gagcagtaat
    33901 aggaacagga agtaaaagaa ggaagtttca attattgaaa caaagacctg atttaaatat
    33961 agtacaagtt agaggtaatg tggaaactag gatacgaaaa ataaaagatg aaaacatgca
    34021 tggtattgtg ttggcagcat caggaataat tagagctaat ttgcaagata aaataagttc
    34081 ttatttacct gttgatgttg ttattccagc acctgcccaa ggagctttag ctatagaaat
    34141 tagaagcaat gatagtacga tagagggaat agtaaattca ctgaaggatg agaatacaga
    34201 aatccaaata ttggctgaaa gaggtttttt agatggtgta aatggaagtt gtcatattcc
    34261 aatggcagct tattgtgaaa taatccaaga taaaatacat cttactggct tatatggaga
    34321 ttcagaagga aaaaaagtag taatcaagtc tatagatgga gatatttctt ctcctagaga
    34381 gttaggttta aagttagcta aacttgtatt gaaggagtat gagaattatg aagggtaagg
    34441 tttacttagt aggggcagga ccaggtgatt ataaactcat gactttaaaa ggtttagagt
    34501 gtataagaaa atcggatgta atagtatatg atagattagc aaatagtagt tatttaagag
    34561 aagctaagcc tgattgtgaa cttatatatg taggcaaggc atcaagtaat catattttgc
    34621 cacaagaaga tataaataga attatagcag aaaaagctaa agaaggaaaa attgtaactc
    34681 gtcttaaagg aggagaccct tatgtatttg gcagaggggg agaagaagca gagactttag
    34741 tagatgaaga tatagaattt gaagtagttc caggtataac ttctgctata ggaggtttat
    34801 gttatgcggg tattcctata acacatagag accatgcttc gtcatttcat gtaataacag
    34861 gtcatttgaa gggtgatgat agtggagaat taaactggaa tgctcttgca aataacaaag
    34921 gtactcttgt gtttttgatg ggaatttcaa acctagagaa aataagtgaa aacttaatga
    34981 aagaaggaaa agataaggat acacctgttg ctcttataag ttgggcaact agatataatc
    35041 aaagagttgt tacatctaca cttgagaatg tttatgaaac tgctattaaa gaagaagtaa
    35101 aaccgccaac tcttatagta gttgggagtg ttgtaggact tagagaaaag ttgaacttct
    35161 ttgaaagtaa gcctttattt ggaaaaagta ttgctgtaac taggtctaga aatcaaaata
    35221 gtgtattggt agaaaaaata atggatttag gtggaaatcc tatagaaata cctactatta
    35281 aggtagaaaa aatacaaaat aatattaact tagaaaatga aataaaaaat ataaataagt
    35341 ataattattt aatactaact agtaaaaatg ccgtagaaat cttctttgaa aagatatatg
    35401 aaatgaattt tgatttaagg atattatcta atttaaaggt atgtgctata ggttctgcta
    35461 catcaaatga gttgaaaaaa agaggaatta tagcggatat agtaccaaaa aaatttgtag
    35521 cagagtcact gtatgaagag ttagctccta ttttaaatga taacgataat atacttatac
    35581 ctagagcaga aaatgctaga gattatctgg ttgacaaact taaggctatt tgtgaagtaa
    35641 cagaagtaca tatatataga acagtaatag atgaggataa aaaaggtgag atattagata
    35701 ttttaaattc taatgatata gattatataa catttactag ttcctcaaca gtaaataatc
    35761 ttgttgaaat tataggaaaa gaaaatatta aaatattaga aaaaataaaa acactatcta
    35821 taggtcctat aactacagag acaataaata gtttgggaat aaaattgtac aaagaatctg
    35881 ctacatctac tatagattcg ttagtagatt taattataaa aaattaattt tggaggcttg
    35941 aatatgttaa gaagaccaag aagactaaga gcaagtaaag caatcagaaa tttggtaaga
    36001 gaaactaagg tgaatgtaga agatttaata tatccactgt ttgtagtaga gggggaaaat
    36061 ataaaagaag aaatcaattc tttaccagat gtatatcatt tatcaataga catgctagaa
    36121 gacgaaataa aagaaataag tgagttggga attgaacata taatgttatt tggtgttcca
    36181 gatgaaaaag atgagtgtgc ttcacaagct tttaatgata atggaatagt tcaaaaggca
    36241 attagaaagg taaaagaaat aaattcagat ataaatgtaa taacagatgt ctgtatgtgc
    36301 caatatacaa gtcatggaca ttgtgggatt ttaactgata ctggttatgt tgataatgat
    36361 aaatcattgg agtatttggc taaaatatct gtaagccatg ctaaagcagg agctgatatg
    36421 gttgctccat ctgatatgat ggatggaaga atacaagcaa taagagaagc tttagatgaa
    36481 aatggattag aaaatgttgg aatcatggct tatagtgtta agtatgcttc aaattattat
    36541 ggacctttta gagaggcagc tcattcagca ccatcttttg gagatagaaa aacatatcaa
    36601 atggacccag caaatagcaa tgaagctata atagagactg aacttgatat attggaaggt
    36661 gcagatatac ttatggttaa acctgcatta tcatatttag atattataag aagagttaaa
    36721 gataattttg acttacctct agcagcttat aatgtaagtg gagaatactc tatgcttaag
    36781 tttgctgtga aggaaggttt attaaatgaa gatgtaattc ttgaatcaat tacatctata
    36841 aaaagagcag gagcagatat aataataact tattttgcta aagacattgc taaactgttg
    36901 aaaaaataaa ttctagtagt ataaagaata tcaaaaagta taggaattgt aattaactat
    36961 tatacagtag ttttgtttag acatttattg aagaataata gaaactaatt tacaatttct
    37021 atactttatt tatgtaacta aataaaattt ggaggtagaa gatgctatat cctattaatt
    37081 taaaattaga tgagttagat gtagttataa ttggtggtgg agaagttgca tataggaaat
    37141 gtaaaaattt tttagagttt aacaaaaatg ttactatagt ttctaaacaa attttaaata
    37201 aattttatga tttaaaagga aatataaaaa taataaaaga tgattataaa gaaatatata
    37261 taaaagatgc ttctgtaata attgctgcca caaataatag agagttaaat atggaaatag
    37321 gattatattg tagagcaaaa aataagctag tgaatgtagt tgataatata gaaatatcga
    37381 actttactgt tccatcatat ataaaaagag gagatttact tataagtgtg tctactgggg
    37441 gcaagagtcc ttcattatca tcaaagataa aaaaagaaat tgaagagaga tatactgaag
    37501 attatgagga gtatttaaat gtattaggag atataagaaa aaaggttata aaaaaatacg
    37561 aagataaaag taaaagaaaa gatgtgctta atatgttggt aacacttgac ctagaagaac
    37621 ttaagaagtt tgatatataa aaaataaaat aaaaaatgtt aaaaaggtgt tgacggatta
    37681 tttttaagat gatatagt
//
LOCUS       DAEPLF010000018        37090 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEPLF010000018 DAEPLF010000000
VERSION     DAEPLF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37090
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.018"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(72..275)
                     /locus_tag="KR314_000797"
                     /pseudo
     CDS             complement(72..275)
                     /locus_tag="KR314_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
     gene            complement(275..604)
                     /locus_tag="KR314_000798"
     CDS             complement(275..604)
                     /locus_tag="KR314_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBH0327197.1"
                     /translation="MPQIKIRGINENDICKISKKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            934..1860
                     /gene="fba"
                     /locus_tag="KR314_000799"
     CDS             934..1860
                     /gene="fba"
                     /locus_tag="KR314_000799"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBH0327198.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(1995..3626)
                     /locus_tag="KR314_000800"
     CDS             complement(1995..3626)
                     /locus_tag="KR314_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0327199.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(4109..5146)
                     /locus_tag="KR314_000801"
     CDS             complement(4109..5146)
                     /locus_tag="KR314_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBH0327200.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(5165..5950)
                     /locus_tag="KR314_000802"
     CDS             complement(5165..5950)
                     /locus_tag="KR314_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH0327201.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(5983..7116)
                     /locus_tag="KR314_000803"
     CDS             complement(5983..7116)
                     /locus_tag="KR314_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBH0327202.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(7164..8291)
                     /gene="hadC"
                     /locus_tag="KR314_000804"
     CDS             complement(7164..8291)
                     /gene="hadC"
                     /locus_tag="KR314_000804"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBH0327203.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKNETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(8291..9517)
                     /gene="hadB"
                     /locus_tag="KR314_000805"
     CDS             complement(8291..9517)
                     /gene="hadB"
                     /locus_tag="KR314_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBH0327204.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(9532..10332)
                     /gene="hadI"
                     /locus_tag="KR314_000806"
     CDS             complement(9532..10332)
                     /gene="hadI"
                     /locus_tag="KR314_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBH0327205.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(10371..11570)
                     /gene="hadA"
                     /locus_tag="KR314_000807"
     CDS             complement(10371..11570)
                     /gene="hadA"
                     /locus_tag="KR314_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBH0327206.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            12247..13245
                     /locus_tag="KR314_000808"
     CDS             12247..13245
                     /locus_tag="KR314_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBH0327207.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            13607..14134
                     /locus_tag="KR314_000809"
     CDS             13607..14134
                     /locus_tag="KR314_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBH0327208.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            14161..14757
                     /locus_tag="KR314_000810"
     CDS             14161..14757
                     /locus_tag="KR314_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBH0327209.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKDVIDTVSISLNAPNKEAYNRVTQPKFGEKSFDYMLGFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(14822..16405)
                     /gene="asnB"
                     /locus_tag="KR314_000811"
     CDS             complement(14822..16405)
                     /gene="asnB"
                     /locus_tag="KR314_000811"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBH0327210.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(16971..17807)
                     /locus_tag="KR314_000812"
     CDS             complement(16971..17807)
                     /locus_tag="KR314_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0327211.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKKFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            complement(17831..19297)
                     /locus_tag="KR314_000813"
     CDS             complement(17831..19297)
                     /locus_tag="KR314_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBH0327212.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIAKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            complement(19300..21162)
                     /locus_tag="KR314_000814"
     CDS             complement(19300..21162)
                     /locus_tag="KR314_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0327213.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            complement(21409..22272)
                     /locus_tag="KR314_000815"
     CDS             complement(21409..22272)
                     /locus_tag="KR314_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBH0327214.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(22327..23259)
                     /locus_tag="KR314_000816"
     CDS             complement(22327..23259)
                     /locus_tag="KR314_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBH0327215.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            complement(23345..23896)
                     /locus_tag="KR314_000817"
     CDS             complement(23345..23896)
                     /locus_tag="KR314_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0327216.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            complement(24125..24616)
                     /locus_tag="KR314_000818"
     CDS             complement(24125..24616)
                     /locus_tag="KR314_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327217.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            complement(24644..25204)
                     /locus_tag="KR314_000819"
     CDS             complement(24644..25204)
                     /locus_tag="KR314_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBH0327218.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            complement(25356..26513)
                     /locus_tag="KR314_000820"
     CDS             complement(25356..26513)
                     /locus_tag="KR314_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH0327219.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRHFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            complement(26550..27491)
                     /locus_tag="KR314_000821"
     CDS             complement(26550..27491)
                     /locus_tag="KR314_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327220.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            complement(27578..28270)
                     /locus_tag="KR314_000822"
     CDS             complement(27578..28270)
                     /locus_tag="KR314_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0327221.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            complement(28380..29192)
                     /locus_tag="KR314_000823"
     CDS             complement(28380..29192)
                     /locus_tag="KR314_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBH0327222.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            complement(29243..30154)
                     /locus_tag="KR314_000824"
     CDS             complement(29243..30154)
                     /locus_tag="KR314_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0327223.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEVGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            complement(30213..30944)
                     /locus_tag="KR314_000825"
     CDS             complement(30213..30944)
                     /locus_tag="KR314_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBH0327224.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            31033..31284
                     /locus_tag="KR314_000826"
     CDS             31033..31284
                     /locus_tag="KR314_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327225.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGTKFINKKR"
     gene            31517..31936
                     /locus_tag="KR314_000827"
     CDS             31517..31936
                     /locus_tag="KR314_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327226.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSPINLKSGDIFTFIRLTLLAGRMF"
     gene            31961..37018
                     /locus_tag="KR314_000828"
     CDS             31961..37018
                     /locus_tag="KR314_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBH0327227.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVTSALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNSDRQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTSEDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKFKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGIDYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDESGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQLVQPFKQVFREYYRINSDEISEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNINFEGSHV
                     LITGNLGEYSVHMGSGIVHKKGMGALNILPVHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDNKIKDPSILSNL"
BASE COUNT        12222 a         6319 c         4454 g        14095 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaagtcac gagtgtacgt
       61 agctattttt taatcaaata tatatattct ctatatataa cttcttctcc attaaataat
      121 atctcatcat tttcagtgtc tacttgagtt cctggatttg aaacttcact tccatttact
      181 actacagacc cttgtttaca atacttcttt atttcacttc tacttccata acctaaattt
      241 gaaagaattt tatctatacg ttgcttttta gccattaaaa atgctctcca ttctcataat
      301 atttttcttt ttcaaatact gtaaatgcta aatccatact ttctacatct aaattatttc
      361 ttatactatc agttattatt cttgctacta tatcttgtac ttcttgtcct ctatcaaacc
      421 aagctacttc aacaaaagga tatacatctg ctattttacc atctcttatt gcaactgact
      481 ttatacattc tatttcaaag tagtctcttg gacattttac agcttcaact aaatcattta
      541 tcattttttt acttatttta catatatcat tttcattaat tcctcttatt tttatctgtg
      601 gcatagttta tctcctttat tttaaatttt tagtgaatta tcatccactt aattcccttc
      661 gataacgttt gaagtggtaa cttcttggtc aagacaacta ttgttatcag tatctccaaa
      721 ctaataaggt agttcctacc cttatagtta gcattatata gctaattttc ctaaattatt
      781 tatattcttt attattttat ttaatcctat tgtcttctat ttaacattat acttgtaatt
      841 tatagttatt acaatttact atatactttt tttgatttaa cgttataata taagtgatgg
      901 gtaaatttta aaagttaata ggaggagttt aaaatggcat taattactac aaaagaaatg
      961 tttaaaaaag cttacgaagg tggatttgct ataggtgcat ttaacataag tgacttagaa
     1021 caattacaag gtgttttaaa agctgctaaa gctaaaaatt cttatgttat gatacaagct
     1081 tctatgtcag ctgttaaata tgctggacct catacattag ttgaaatggt taaggctgct
     1141 tctgatgaaa taggtgtaga tgtagctctt cacttagacc atggtccaaa tatggatgct
     1201 attaaaactt gtattgatgc tggtttctct tcagttatga tagatggttc tcattttgac
     1261 tttgaagaaa atgttagaat aactaaagaa gctgttgaat atgcacactc taaaggtgtt
     1321 gttgttgaag cagaacttgg tgttctagct ggaacagaag atgatgttac ttctgatgtt
     1381 cacaagtata ctcaaccagc tgaggcagtt gagttcgttg aaagaacagg tgttgattca
     1441 ttagcaatag ctattggaac ttctcatggt gctttcaaat tcaaaggaga agctaaatta
     1501 agatttgata tactagaaga aatccaaagc aaattaccag gtttcccaat agttcttcat
     1561 ggtgcatctg ctgttgacca aaacgctgtt gctacatgta atgaatttgg tggaaatata
     1621 gctggtgcta aaggtgtacc tgtagatatg ttaagaaaag catcttctat ggctgtttgt
     1681 aaaataaaca tggatactga cttaagacta gctatgactg ctgctatcag aaaattctta
     1741 gctgaaaatc caaaagaatt tgacccaaga aaatacattg gtgctggtag agatgctata
     1801 caagctgtag ttgaaagcaa gatagatgat gttttaggtt ctgctaactc tataaactaa
     1861 tataactttt aaatatttat aaaagaaaag agatgactta aagtaattaa atttattttt
     1921 aagccatctc ttttaagttg tatcaattat gtatcttata atattttaat tatattgtct
     1981 ttttaaactg acagctaaac gtgaatatgt ccactcttta tctttctatg gatggtagaa
     2041 ggatatatac ctataacttc agcagccttt gttattgaac catacttatt tatagcctta
     2101 cttatcatta tttggtccac tattttatat gcatcattaa gattcataat tccatttacc
     2161 actattggtg ataaatcatc tgtttcatta tcaatcatat ctaaatttat cagcatgtta
     2221 aattcatctt ctccaattac atttttagct gataatacta caaatctttc aatgatgttt
     2281 tttaattctc ttatgtttcc tggccaagaa tattcttcta ataactcaat aactttaggt
     2341 gaaacttcta cttctctatt gtacttttca ttgtaaagtt ttaagaaata atttactaat
     2401 ggtactatat cctcttttct ttctctaaga ggaggtattt ttactggaat aacacttaat
     2461 ctatagtaca aatcttgtct aaatttaagg ttatttctaa ggtcttcatg agaaatatta
     2521 gtagcactta tatatctaac atctattttt ataggcttac taccacctat tctagtaata
     2581 gtattttctt gtataaccct aagtaatttt ttttgcatct gcattggtaa ctcacctatc
     2641 tcatccaaga aaatagttcc accattcgcc tcttcaaaaa gtcccttttt accttttttc
     2701 ttagctccag taaaagaacc ttcttcatat ccaaaaaact ctgactctaa taattcattt
     2761 ggtatagctc cacagtttat agctataaaa ggtttgtctc tccttgaact atacttatat
     2821 attgttcttg ctattatttc ttttcctaca cctgattctc catttataaa tatagaagaa
     2881 tctgaggctg cagctctctt aacaactttt tttatattgt ccataatctt actttgactt
     2941 atatactctg tgtcttctga tatatcacta tattttatct gagattcaag cttaataacc
     3001 cttgaaatat ctctccctgt tagtactcct cctgtaagct ttcccttaga atcccatctt
     3061 ggaactgccg tatatgcaat aagcttccca tctgggtaaa cgatatttct ataaatcttt
     3121 ttcttcactt taagcatgtt taatattatt ggttcttcaa ctaatccttg tctcacaagg
     3181 tctgttatat ttctaccaag tacctcagtt cttttaaaag gtaatatctc atcactgaca
     3241 gtgttcatct tttcaattac cccatctttg ttaaacacaa caacaacttc atctatatgc
     3301 tctattatat cttcaattac tttatcagat attagagact tctcaaataa ataaactata
     3361 ttattttcat tattattatc tttcatattg tatatatgtg cagtcatcct taactctctg
     3421 aaagctattt tctttttact atctgtcagt atttctgttt tgtcaaaatt tgaatctaat
     3481 tcatttatat ttctaggctt ttttatatct agaaaactaa aaatactatc tattagatga
     3541 ttacagtata ttatttcgcc tgattcttta cataaaatgg ctggattttc tacatgttct
     3601 ataagcccta ttaaattatt atccattaat attcgcacct ttcattaagt aatttattga
     3661 actacataca aaatttattg tttgcttaca ataaatacca tcagcacata taatcaactc
     3721 cttataatat tcgtcttttc aagtagttta tttcattaaa acgtaactaa gatataattt
     3781 taatgattat agtttgttaa aaatttcatt ttgcttttaa ttgtatttta tcacatattt
     3841 agtaattatt tgtctattta atacaattta attaaattta agatttattt aaaaaaacaa
     3901 agataatcct tacagaatat aactcacaat ttgtaagcaa tatacatata aggattatcc
     3961 agattatctt gtattataaa attttattca aacagacttt ttaataactg acaaatacac
     4021 actagcaata acttatcaaa gggttaataa ttatgttact aactaataag acttattcaa
     4081 tcagtaatat ttatatagtt tgtatctatt aagcttttat agcttctaat aaatcatcaa
     4141 cgctatctaa tttttctata agcattggaa ctatttcatg taagtctcca actactccat
     4201 agtcagcgat ttctaaaatt ggagctgctg ggtttttgtt tatagcaatt atgaaatctg
     4261 atgattgcat acctgctaaa tgttgtattg ctcctgatat accacaagct atatataggt
     4321 ttggtttaac agtagttcct gtttgaccta cttggtgaga atggtctatc catccagcat
     4381 caacagccgc acgagaagaa ccaactactc cacctaattt gtcagctaat ttcttaagca
     4441 tttcaaatcc ttctggtcca cctaatccta aaccacctga tactataaca tttgcatctg
     4501 taagagatac taaatctttc ttagttttaa ctgtttctaa aacagtagtt cttatatcat
     4561 cttgagttat tttgtagtct aatgctataa cttcaccagt tcttgtttcg tctttttcag
     4621 ctttatccat aacaccaggt ctaactgtag acatttgagg tctatggttt ggacaaatga
     4681 ttgtagccat tatgtttcca ccaaatgctg gacgagtttg ttttattttc ttatcttctg
     4741 ggtctatttc taacttagta cagtcagctg ttaatccagt tccaactctt gaagctattc
     4801 taggtgctaa gtctctacct atatgagttg ctccgaaaag cattatttct ggttttattt
     4861 catctattgc atcttttatt acttttgtat atgcatcagt tgtatatttt tctaaaagag
     4921 catcatctgc aacataaact ttgtcagctc caaaagtgat taattcttta gctaatccat
     4981 caacatcttt tcctaataat attgcacaaa gttctgcatc tacttcttta gctaattttc
     5041 ttccttctcc taataattct ataactactg gagctatttt tccttctctt tgttctgcaa
     5101 atatccatac gtttttataa gaacttaaat cttttatatc attcatagtt tgtactcctc
     5161 caacttatat aatgtgcttt tgttttagac cgattattat tttactagct atttcatcag
     5221 ctttaccttc taagatttct ccttttcctc ttggttcagg agtaaatgat ctaaatactt
     5281 tagttggtga tgcttttaaa ccaacttctt ctttatttac atctaaatca tcaattgtcc
     5341 aaacttttac atcatgttta tatgctttta ctattttatc aacagacatg tatctaggtg
     5401 tatttaactc ttttactgct gttaaaagta caggtgtact tacttttatt aattcgtatc
     5461 catcttctaa ttgtctttga actattatat cttttccttc tattttgaaa tcttgtacat
     5521 atgtaacttg aggaatgtct aatttctctg caatttgtgg tccaacttgt gcagtatctc
     5581 catctattgc ttgtcttcct gcaaatatta tgtcatagtc acctacttta gatattcccg
     5641 ctgcaatagt atttgatgta gcccaagtat cagctcctcc aaatgctcta tcacttaata
     5701 atattgcatc atcagcaccc atagctaaac actctcttag cataaagtct gcttgaggag
     5761 gtcccattgt tataactgtt atagttacat catcatgctc atctttaatt tttaaagctt
     5821 cctctaatgc atttgcatca tctgggttta atatacttgg aactccatct cttataagag
     5881 tacctgtttc tttatttatt cttacttcgt tagtatctgg aacttgtttt acgcaaacta
     5941 atattttcaa agtctatacc tccaatttca ttagattatt tattatttta atacgtttga
     6001 tgaaattacc attttttgaa cttctgatgt tccttcgtat attgatacga ttctagcatc
     6061 tctgtacatt ctttcgattt cataatcttt tataaatcca tatccaccgt gaagttgtaa
     6121 tgctttataa gcaacttcat ttgcaatttc tgatgcatag tatttagcca tagcagattc
     6181 ttgagcaact ctcattcctg catctctctt ttgtgctgca tcataaacta atcctctagc
     6241 tgcacaaact tttgtttcca tgtcagctat agtaaattga gtattttgga atttagctat
     6301 aggcttacca aattgaactc tttgttttgt atatttaaca gcttcatcta aagcaccttg
     6361 agctattcct agagctaagg cagcaacacc tattctacca acttctagag tacccattgc
     6421 tatcttaaat ccttgacctt cttttccaag taagttttct tttggaactt taacgttttc
     6481 aaatattaag tcagaagttt cagttcctct tatacccatc ttatcttcat gagctcctgt
     6541 tgagaaacca tcccatttac tttctactat aaacgctgat gttcctctaa gtcctttact
     6601 tctatctgta actgctatta ctatagctac atcacagaat ggtccattag ttatgaaagt
     6661 ttttctacca tttaatatat agtggtctcc aactaattct gctgttgttt gttgtccacc
     6721 tgcatcagaa ccagctcctg gctcagttaa cccaaatgct cctattaatt cacctgttgc
     6781 tatacctttt aagtattttt gtttttgctc ttcagtacca ttgtaaagta ttggtaatga
     6841 agcaagagaa gaaccagctg taacaaaagt agctgttgaa gcacatcttc tagctatttc
     6901 ttccattact atagcgtaag aaacatatcc tgctccccca ccaccatatt cttctgggat
     6961 attactgctt ataaattttg catcagctaa ttttttgatt aattccttag gcattacacc
     7021 tgttttatcc atttctgctg gtaaagtatc taattcttta ctaacaaaat ctcttactgc
     7081 ttttcttaaa agttcttgtt ctttgttata taacatagct tcctccaatt ttcatatttt
     7141 atagtaaatt tattttaatg aacttacgct aaactcatca tctcagcaaa agtttgaata
     7201 cgagttctgg cttgttcatt attctgagtt tgttggtcga tttcaacata taaagtaggt
     7261 attccactat cttctatatc ttttctaact aaaggataat cgtattcttc tgggtcacag
     7321 aatttcatca tacagaagat aacaccatct atatctttct ttttaacttc atctactata
     7381 agtgacccac gttttttctt agggtcataa gctaatgaac atccttctat gtttgaccat
     7441 tgtcttgcta atctctctaa cgcatcatca cctgctggta catctgttct gaattgtctt
     7501 gtttcttgtg ctaagtcgtc agctacaact gatatattgt tgtcttctaa aatgtctaat
     7561 atatctttag aatcagctaa tatacctgtt aataaaactt tttttccaga acagacttct
     7621 tctggcatag catttaattt tgctattaaa tctttcacta gctcagtatg ttcttctttt
     7681 ctcataaaga acccactttt aattactaag cttcttattg atggttttat agtattagaa
     7741 tgtttataag ctacttcaac aaagtctctc atagtttttc tatgttcatt gtaaacttct
     7801 atactttcat gaatttttgc ttcttctatt tcatatccac aaatttcttc aagttctctt
     7861 tttacacctt tgtactcact gattaagtat tttacaccag cttcaagttt tctattttgt
     7921 gggtgtacta atgatatata ttttatatga ggtactgctg atttccagtt ttgtcctaaa
     7981 caaattagtg tatcacacat acctggtata ataactccag ataattcatc ataagcacct
     8041 tttaatccat attctaaaca tgattgcatt attgaacatg caaatgcagg gaaatattgt
     8101 ttagctaaat ctaattctgt ttgtcctccc catataccaa ctggaagcat tccagctgca
     8161 tgtataattt cttctgggca ataaactggg aaacaaccta tagctttttt accagtttca
     8221 tttttatatt tttttacagc cgcatttgga ttttcaacta cttctttcat tttagataaa
     8281 atagcttcca ttatatctca cctctattaa gtttttttct ttcttccatt acttcaacta
     8341 aaccttgaat tcttgtttca aattgagcat tagtgaaagc tctagggtct gcttggtcac
     8401 catcaaatcc agcatatggt aatccagttt cttcagctgc tcttctttgc atttcatatt
     8461 gtattaaact cataagttta cagcttctgt tcatatgata gaatgctcca tcacatttac
     8521 cctctactaa agaatcaact ctatattttg tcatacggtc taggtttaca ttgttaaaca
     8581 tagtactata tgctactgcc attccatcta aatcattaac ttcatattgt aatgcccaag
     8641 catgtgggta aacactacct gtcatgttaa ctccaaattt agctaatgtt ttcatcttgt
     8701 atcctatata tggccaacaa ggtatacctt ccatcattat tctgtatttt tcttcccctc
     8761 tgaaagaaga tttaccagtt ttcatgttgt cttctaattc ttctataagt aacttaaatg
     8821 cttctgtagt ttctttttta cctctagcac aaactattac agccatgtaa gtaaataagt
     8881 caaatccatt cattggagaa ggagaagaat ctgctggtaa actcatagaa tacttccata
     8941 gtcttccgtt ttcagctgat attttcatta cttcttcaaa cttcttaggg tcaaatttct
     9001 ttcctgatat aatttctagt tgttttatag cttcttcaaa ttgagcttta atataatcta
     9061 ttcttgattg agtaacttcg tcttcattat tgaaagttgt atcaatcatt attaaaggta
     9121 tatctaattc tcttgaaata ttttcatacc attttataac ttgattacat atattgttac
     9181 agcaaagtag gaaatctgga gctggcatat ccaaagcttc acatccacca ttttctaaaa
     9241 gaccaaaatt tgttcttgca tatgcacata ggtcaataga atatccttta gattcagcta
     9301 tttcacataa ttctaaagaa ccttttttag ctgctactcc agctgcttgg ttttctggat
     9361 ataatacgtt taagtcaaat acttctgcta actcttgagg aaatactgat gttgaccaac
     9421 ctacaggtct tccttcttct ttagctttaa atgcatttgc atattgttca gctaataaat
     9481 cattaattac tactctagct tctttttttt cagacatttt catccctctt tttatatatt
     9541 tttcacttct ttttgtgatt cttttgcttc atcaaaagca tataacgctg ctcctaaagc
     9601 accagttaat tgaggtatat caggtacaat aatttctgtg ttgatttctc ttgccatagc
     9661 tcttacaata ccactatttc tagcaactcc accaaccata actacatttc tttgtactcc
     9721 tattcttttt actaggctag aaactctctt tgctactgaa gtatgaatac ctgccactat
     9781 atcttcaatt tttgcatttt cagataaatg tgatataacc tcagactctg caaatactgt
     9841 acatgtactg cttattgata cttcattttg agaattcata gatatacttc cgagttcaga
     9901 tacatcaacc tctattattt ttgccattac atctaaaaat cttcctgtac ctgcagcaca
     9961 tttgtcattc ataagaaagt ttaatagtct tccattatta tctaatttta atacctttgc
    10021 atcttgacca cctatgtcaa taatggttct tgtctctgga attatgaaat ttacccctct
    10081 agcatgacag cttaattcac ttatttgctt atcagcatct gaatagttca ttcttccata
    10141 tcctgtaact acaacttttt taatatcttc tcttgcaaga cctgtcttgc catataattt
    10201 ttctaaaact cttgatggtc cagtagtccc agtaccagag gatattgttt cagaagctac
    10261 aatatcttcc ccattcttta agattactcc ctttgatgca gttgaaccta tatctaatcc
    10321 cattgtgtac atctttagca ttctcctttt aaaaaatcta gaattactaa ttaatatctt
    10381 acaactttac tatctttaaa gttatttatt ttctcttcat cgtagcctaa agattttaat
    10441 acttctacag tatgttgacc tacttttggt gctggtgtat attctttaat tccttcattt
    10501 ctaaacataa ctggagtatt aactaagaca cctgtatttc cgctatcgta tgttttctta
    10561 aataagaagt catttgccca agcttgttcg tcatctaata aatcttcaca gctttgaatt
    10621 ttttcaaatg gtaagtctgc ttcttctaat aaagctgacc actcgtctaa tgttttttct
    10681 aacatagctt ctccaactat cttaactaaa tcttcaacat gattaaccat tgaatctatg
    10741 ttattatatc tatcatcttc taatatatat tctctattta taaccttaca gaatttgcct
    10801 aaccacttgt tgtattgtat taaagctaat tgaatccatc ttccatcttt acatttatat
    10861 gtagtcatta atgggctgtt tggattttct cttgataaag gcatttcatt tccgtattgt
    10921 gctgttgtta tcattgttcc cattccatat atagctgtat ggaaaagact tactgttact
    10981 ctctcacctt taccagtttg agctttttta tgtaatgctg ctaaacttcc tgctgctagt
    11041 gctagacctg catagtggtc accaaatcct gctgctgtat ttgctggaga tgttcctttt
    11101 tccataacag attggctaac tcctcctctt gcgaagtatg cagtatagtc aaatcctggt
    11161 ttatctttta aaggtccttt ttcaccatat cctaatattt gagagaatat taatcctgga
    11221 tacttatctt ttatttggtc ataagctata cccatttttt ctaatgcttg aactctaaca
    11281 ttagttacaa atatgtctgc ttctgataat aatttatgta atatttctac tccttctttt
    11341 gattttacat taatacttac acccttttta tttccatttt ctaattcaaa catagggttt
    11401 tcatcatctg atgctggaga tttaaatgtt ccacccataa ctcttattcc atcaccttct
    11461 ataggttcaa tcttaataac ctctgcaccc cagtcaccta acatttttgc acaacatggt
    11521 gctgcgatga aacttgaaag ttctactact ttaactcctt ctaaaagcat aacgttttcc
    11581 tccttaatag tatcaattat aaaatttcga ttaaatcact cataaaatac ttttttctga
    11641 tattaactgt ctgatgtcaa agttcctaat cttatctaaa ataacttaaa aattattaaa
    11701 attatttttt attgttttat tgtcttacac ctatattgtt aagcatttat cgtgccaact
    11761 ttatatttaa tcaaaatatc gcttgatttg aatgtttttt aacaatatgc aattgaataa
    11821 tctttttcat atcgtggcat cttgccacta tccattgcat tttgctattc tttctatgct
    11881 tataaaagtt ttttttatat atttttttat gaaaaagctt gacatttcaa aaaaagtttt
    11941 cattgctaaa ctcaaaaaca catgttatca ttgtaattaa tcagcaagtt agttaactaa
    12001 tttcaaactt agataatttt ttaataatat ttaatctaaa aaataacaat ttctttgata
    12061 aattttagag taattattac attaaataaa aaattactaa atatcaaaat aacacaggga
    12121 aaacaatttc aaaactaaat ttttaacctt aaaaattttg tataaaaaat ataaccttat
    12181 atgctacata ttatttctaa atgaaaaatt accataataa tttaataatt acaggagggt
    12241 attgatatga aaatactagt atttggagca cgcgattatg aagaaccagt aataaaaaaa
    12301 tggtctgaag aacataagga tgttcaagtg gatatttatc ctgaaaacat gactgaagaa
    12361 aatgtagtta aagctaaagg gtatgatggt atatctatac aacaaactaa ctatatagat
    12421 aatccttata tttatgaaac tttaaaagat gctggggtta aagttatagc ttcaagaact
    12481 gcaggggttg acatgataca ttttgattta gttaatgaaa atggacttat cgttacaaac
    12541 gttccttctt attcacctaa tgcaatagct gaattagctg ttactcaagc tatgaacctt
    12601 ttaagaaaga ctcctctagt aaagaaaaaa gtctgtgaag gtgattaccg ttggatagct
    12661 gaacttcttg gaacagaagt tagatctatt acagttggtg ttataggtac aggaaaaata
    12721 ggtgctactt ctgcaaaatt attcaaaggc ctaggagcta atgtaattgc atttgaccaa
    12781 tatccaaata gtgatttaaa tgatatatta acttacaaag attctttaga agaccttcta
    12841 aaagaagctg accttataac attacatact cctttacttg aaggaacaaa acatatgata
    12901 aataaagata ctctagctat aatgaaggat ggagcttaca tagtaaatac tggccgtggt
    12961 ggtttaatta atacagggga tttaatagaa gcactagagt caggaaaaat tagagctgct
    13021 gcccttgata catttgaaac tgaaggattg ttcttaaaca aaaaaatgaa tcctggagaa
    13081 ttaactgacc cagaaataaa taaacttctt tctatggaac aagttatatt cactcatcac
    13141 cttggtttct tcactagtac agcgattgaa aatatagttt attctagttt aagtagtgct
    13201 gtagaagtta taaaaacagg aactgctact aatagagtaa attagtattt tataaatata
    13261 ttttttcatt aattagctat tatctaacaa aatagccttt aatttcccaa atataagaga
    13321 gtcagctcag tatatattga gtagactctc ttatattatt ttttatatta aacttgtact
    13381 cctcttattt ataccaaatt aaatttatat taacttactc taactactcc tactctatac
    13441 attttaccta tttttacttt tctaattata tacatttgaa tgggtacaat ctgtatttta
    13501 caaaaaaaac acctaacaaa ctatgaaaat tgtatgatta atattaatat atttataaga
    13561 aaacataaca attatgttag ctctggattt ttagggaggg agtatattgg aaaaaatgaa
    13621 taaagttact tctaaaaaaa ctacctatat tgttacttca gcattatttg catccataat
    13681 atgcctgaca atagcatata tattacatat tccagttggt ggaaacaatg gatatgttca
    13741 tataggagat gcttttattt atctagctgc aactattttg ccaacaaact atgctatagc
    13801 tgcttctgca attggtgctg gtctagctga tttatctaca ggtgctgcta tctgggtaat
    13861 accaactata atcataaaac caatacttgt attatttttc acttctaaaa gtgacaagat
    13921 aataaataag agaaatatag ttgcttcggt tgtagcaggt atagtaggat tagttttata
    13981 catgtttgct gaaggtataa ttattggaag ttttacaagt gcatttgtaa tgagcttact
    14041 tggcttatta cagccaatag gaagttttat tgtttttata atcttaggta tggcacttga
    14101 taaacttgat tttaaaaaac gttattttaa ttaagaattt aaatttatgg agggaaaatt
    14161 atgaatatct tatatacaat tgaaaatagt atctatgtaa atataactaa tacatgtcca
    14221 tgtagttgtg ttttttgtat aagaaacgaa aaagatgaag ttgcaaatag tggtagttta
    14281 tggttggagc atgagccaag tgtagatgaa gtaaaagaag ccttcaataa atacaattta
    14341 gacgactatg atgaaatcgt attttgtgga tatggcgaac cattaatgag aataaatgaa
    14401 ttgatagagg tagcaaagtt tataaaagaa aaaagtagca taaaaataag aataaataca
    14461 aatggtctaa gtgatttaat acataacaaa aaaacagcta ttctgttaaa agatgttata
    14521 gatacagtat caataagtct taatgcccca aataaagaag cttataatag agttactcaa
    14581 ccaaagtttg gagaaaaatc atttgactat atgttaggtt ttgcaagaga ttgcaaaaaa
    14641 tatataaaag aagtagcttt ttcagtagta gatgaaatat ctccagaaga aatagaagaa
    14701 tctaaacaac tggcaaaaaa acttgatata ccattaagag ttagacataa gaattagcaa
    14761 gtaaaattat aaaacattaa gaatcacctg aatggtcttt caggtgattc ttcctcttgt
    14821 attaacattt tacaaaatag atttcatacc acactaggaa tgtgtaaatc atccaaatct
    14881 ttctccaatt atcagagttt ccactaatgt agtcattaaa aatctcatta atctcatcta
    14941 tgttaaagaa tttatctgct atctcactat tgaatttatc tcggatatcc ttattatatt
    15001 ggtcatcagc catccatata cgaattggca caataaatcc taatttattt ctaaaagcaa
    15061 tctcttctgg taatgttttt gatgcagctg tacgaaaggc aattttattc tcttttcctt
    15121 tcactttata ttctgatggt attctggatg caatatcaaa tattcttgta tctgtaagag
    15181 gcattctgac ttccaatcca actgcagtac tcattttatc tatattaaaa taaatatctc
    15241 cttccaacca tatttgaata tctacatttg acatgttagt aagtgtatct agttcttctg
    15301 tttcttcata aattcctttt accacttcaa taggaagcac atcaggattg tatttcttaa
    15361 gaatttttct tttctcaccc tcattcataa tgtttgtatt tccaatataa aatgtcttca
    15421 aattctctcc ataacactct gccttatggt atatattata tccaccaaaa aactcatctg
    15481 ctccttctcc agagtaacag atatttgtgt tttcagctgc tgccttgcat ccaatagcaa
    15541 aaacgatagc agatgcatct ccagttggtt gctccatatg gtacattaca taaggtactg
    15601 acgcaaaata ctcttcaggt gtaattatat atctcgaatt tttacaccca agaatattag
    15661 ctgtctgttg tgctaatttt gattcatcaa aacgctcttc ctcataacca catgaacctg
    15721 ttttttctat atctgacatt gccaatatat aagatgaatc aacacctcct gataagaata
    15781 actctgcagt ttcattctct gattttacct ctggcataat tttctgaagt gttgtatgaa
    15841 tttcatccgt ccattcctca atagacttgc tgtggtcagg atgaaattca ggtttccaat
    15901 aacgaccaat acatactgtt ttattctgat agacaaggta atgtcctggc ataagcttct
    15961 ttactccacg gaagaatgta ttttcacctg ctacatatgt gaaagacata taaatctgta
    16021 acatgtcttc atttaactct ttcacaaatc ctggttgctc cataatctta cgaatagatg
    16081 aaccatagag aagcttccca ttttcagtct cataataata gaaaggtttt actccaaact
    16141 ggtctcttag gcagaagagt ttcttttcca cagtatccca taatgcaaag gcaaacattc
    16201 catgcatgtg atttgccata tcatatcccc atgtcttata tgcttccact aaaattgctc
    16261 gttcacgctc atctctacat aatgaagaat cgattccaag ttcttcacag agaactttcc
    16321 agtttcgaat atgtccattg tattctagaa tttttatcat atatgtgtct tttcgtgtta
    16381 aatacttttc ttctaaattt atcatatgtg tatcctttcg tattaaaaca tttaacttct
    16441 aaatttatta aatgtttata tatatttatt cacttataat cactgaaaaa tcgacttttt
    16501 tcaaagtaaa ttctttttga attagtacta atctgctctt tattaatggc ataaatatga
    16561 ttatcaagta ttatcagtgt tataacattg aattttttat tcttcataaa tattgccccc
    16621 tttatattac caactaaata taatatatct tatgttactt acatattctt taagaaattc
    16681 tgaagatttt ataaagtttt ttgtattata attaattaaa aataaaattc tagttataaa
    16741 acatattttc cattaaacaa aaaaatagtt aaaataaatt cataaccaca taaacttatt
    16801 ttaactattt gtatatttta attataaata ttaacttaaa tttttaacag gatacactca
    16861 taaaataaac ataatgaaca acaaaataat atgtctcagt ttctttatat actttacctt
    16921 ctttcaattt tctaccatgt agacgtataa gataatatta gtaattcact ctattgctta
    16981 actcgtgcga tatggacaac caagaaagct atttcctcat ttgttaactt ttttccatat
    17041 ttcttttcta aaaaatctct aacttttaat gcaattaaca tttcttttgg atttgacctt
    17101 ttcatttgat taaataataa atcatcacca ctgcttaaca tactatctga aaaaaatctt
    17161 tccacaaaaa attttatatg tgttataaat ctcatataat gcatactctt tttatctaag
    17221 gttctattca aagaaaaaac aaaaatatta attatttctc caattacttt ggcatactta
    17281 atcgtatcat atgaatttcc atctgaagcc atagcatttg caaaatggaa agcaatgtta
    17341 gctgcttctt cctctggtaa ttgaatgccc aatctctcct ctaccatata aattgcaacc
    17401 attccaacct taaactcatt tggataataa ttttttattt cccaaaatac tcgatttgta
    17461 atttttattc ctttacgcat cctttcaatt gcaaagttaa aatggtctgc taatattaag
    17521 tataagtttt tattaaaaga tgagttcaat aacttttctg cctctactat tatttcctgt
    17581 gttatcttta aaatgtcgac aggaatatca tccattaatt ctaaaaattt ttttgatttt
    17641 gtattttcaa cagggacaaa catttgatta tcattggaac cacaaatata ttctcctgtt
    17701 tttttaccat atccaattcc tttccctagc aaaataaatt cattatactt tgcatcatga
    17761 actaaaacaa cgctcgagtt caaaactttc tttattatgt agttcatttt atttgctcac
    17821 cttctattgg tcatttaaaa tctatactat tattttcaat tactttttta taccaataaa
    17881 aactatcttt cttatatcga ttatatgtcc cctttccata atcatcagca tccacataaa
    17941 tatatccata acgttttgac atttgtttcg ttgattcact gaccaaatca atacaacccc
    18001 atgaagtgta tccccaaagt tcaacgccat cttcgataat cgcatctaac atacatttaa
    18061 agtgttcttt taaataatta attctatatt ggtcatgaac ttttttatct tcagttaaaa
    18121 tatctttagc tcccaatcca ttttcaacta taaataaagg ctttctgtat ctatcatata
    18181 attcaatcaa agatatacgt agacctattg aatcaatttg ccaaccccat tcactcattt
    18241 ctaaataagg attttttaca gctaataacg tattcccagg tgtaatatct aatccagttg
    18301 tatcagctgc ctcacaagaa gacatatagt aagagaatga aataaaatct actggatact
    18361 ttttcataat ttcatcatca tgttcttctt ttgctatttg aatgttatta tttttaaaat
    18421 acgaaagtaa atatactgga tactcaccaa aaacctgcgt atcactatat gcataaatac
    18481 ttctcatttt ttgttgagtt gccaaaacat cttctggtct acatgtatat ggataatacg
    18541 ttaattttgt taacatacac cctacttttg aatttggaat aatctcatgg caaatttttg
    18601 ttgcaagggc actagctaca aattgatgat gcatagcttg aaaaataact tcctcaaagt
    18661 ttttatcctt aaaacggtct cgaactaaac caccagtagt gtacggatga cgaatcatag
    18721 aatcaacttc attaaatgta agccaatatt tcactttgtt tttatatctt tcacagatag
    18781 tttctacata cctaacaaac atattgataa cttgtcgtga ataccatcca tcatagttta
    18841 aaacgatatt taatggtggt tcataatgtg acatagtaac aagtggttca attccataac
    18901 ttatcaactc atcaaataca tcatcataaa actgtaaacc ttcttcattt ggctctttat
    18961 catctccatt tggaaaaata cgagaccaag caatactcat tcgatatgtc ttaaatccca
    19021 tttctgccat tagagcaata tcttctttat agtgatgata aaaatcacta ccatgacgtt
    19081 ttggataatt tacttcatct tcaggatgtg ctaatccttc ttctatatct tttgttgtga
    19141 tttcattatt agatttatag tctttcacat ctgcatcaaa gtgtgccttt gctacatctg
    19201 atacagacca ccctttacca cctaaattcc aaccaccttc aaattgatta gcagctgtag
    19261 ctcctcccca aagaaatcct tctggaaact ttctcatatt tatattcctc cctttacatc
    19321 aaacaagata cttccttttt ttacattgcc ataagactgg tttaacactt tataagcata
    19381 atctgctcca ttattgttta caatcataac agttgaatct aaattagcat gtgagatttt
    19441 gtctaaatca aattgaatta ataaatctcc ttcttttaca ttatcgcctg tcttaacaaa
    19501 tgttttaaat ccatctccat tcatacttac agtatttatt ccaatatgaa tcaaaatctc
    19561 agccccattt tttgttctta aaccaattgc atgttttgtt tcaaataaca tcacaacttc
    19621 tgaatcacaa ggtgcgtata aatttccatt ggtcggtata attgcaactc catctcccaa
    19681 agtcttactt gcaaatagtt catcattaac cttacttaaa tctattactt ttccttctac
    19741 tggagttact aagatattta cttctttttc ttcttctagc tcttcttcaa caacatctcc
    19801 aattgatgac tctactgtat ttgtatcttc cttccaaata atcagtgtaa taataaacga
    19861 gcctaatatt gcaataacaa aaccaatact cgcaaaaatc aaattgtttc cattgtttgg
    19921 gtcaataaac atactcatac tggcaagccc tggtccaaca attacaaatc cttttactgc
    19981 aaataatcct aaaaatgtac cagaaattgc acttgagaat acaacaccta acattgctct
    20041 tttatgttgt aaagtaatac catataaagc tggctctgta attcccatca aggcagaaat
    20101 tcctgcagat aatgctgttt gttttaatgt ttcatctttt gttcgcaagg ctactgcaaa
    20161 acaagcacca ctttcagaaa tattgtgggc taatgaagat gtcatatata aagcttcata
    20221 acccaacttt ccaattgtat taattgaata aggtattagt gctttgtgca ttcctgttgc
    20281 aaccataaat ggaagaaaag cagcaagtaa tcctacaact aagaatccca ttttgttata
    20341 taagaataaa ataatggttg taaaaattgt accaacgtta tatcctaaag gacctaaaat
    20401 agttagagta attggaacag taattgccaa agacatcata ggaacaaaga aagttcgaat
    20461 tggttttggt gaatacttgt taaaaatttt ctccattgtt gccaaaaaag acacacataa
    20521 gatagccggg aaaacttgtg cattataggc aatgtttgga attgtaaatc caaatagttc
    20581 gacaccttca cttaaagcag ttgttgttgc tggaagcaat aagtatccta cagcggcaat
    20641 agctactaaa cgattacatt tcaatacatt ggcagtagtt acagctacca taagaggtag
    20701 gaaatagaat gttgcatctg aaatagcaac taataatgta tatatagtag aatctgattt
    20761 taaaatatta aacattgcta ataacatcaa cagtgatttt aaaataccag ctcctgcaat
    20821 agcaaaaatc agtggttgaa aaatattaat aataaattct ataaatcttg ttcctaagtt
    20881 tattttttct tcagttttta cagtactatc atttgtatct aaagggcaaa cttttaaaat
    20941 ttcatcatac acatcaacaa cactatttcc aatgacaact tgaaattgtg ccccattaac
    21001 tatactcata acaccttgta ctttttttaa agcatcctga ttgactttac ttctatcaat
    21061 caacttaaag cgtagtctag ttgaacagtg ttctaaagct aaaatatttt ttgaaccacc
    21121 aacttgttca ataatgtcaa tggcagtttt tttataattc atcttgtaat ctccttctcc
    21181 ataatttttt atgaatggcc attcaagatg caaaaaaggc agaacctata taaacacctt
    21241 gaaaaatcaa agcaatatat tcgatcctgc ctgcattacc agtaacatgt cttctattta
    21301 tactattttg tattattata ttaacatttg cctaaaagaa ttgtcaatac ttttttaaat
    21361 tttctgatac taactaatag cacctttata attcattttt tatgtcaatt aaagtaacag
    21421 tagccaccca ccaattagta tcaaaattga acctggtatt ttttctttta tattttcttt
    21481 gaatataaaa tgagcaagta ttaaagttat tatcatagat acttgagaaa atggctctgc
    21541 tatactaagt tctagattat ttaaagcaat taataatgct aaataagaat atccattaat
    21601 agcaccactt aaaattgaaa gccccttttt attactaaaa atctcacaaa ttattttttg
    21661 tttcttttta aatgctaaaa agataaataa atttaaacct acgaaaaaat acagtatagt
    21721 tgaatataat ataggagata cttgtacaaa aaaagcctta tccaatactc ttcctactga
    21781 ctgcaaaaat atgtataaaa acattaatct acatggtaaa ttgttaaaaa ttacagagac
    21841 attctttaaa aaagagctta ctttttttaa tataaacact ccaccaatca taattatgat
    21901 tcctgcaact tttgctaaac ttaaaccttc acctaaaaat ataactgata aaagcagtaa
    21961 aaacaatgaa tttaaactat ttattggagt aacaagtgaa acttccccag ttgctaaaga
    22021 cgttacatac gctactgaac caattgaata gactaaacta cttatataac atggtattaa
    22081 aaacgaccaa tcactaactc tatccattat gctaaatgga agtaaacata ctccccctat
    22141 aaagaaaaat agaaatgatg aagctatatt tttataaata tctccttctt catcacctaa
    22201 taatcttaca actactcttt caaaagctaa tagcactact ctaattaata atgcaaaata
    22261 agataatatc atttttgacc tcgcttaaaa attaatccga cttattgtta tattaactgt
    22321 ataagactac catataggta agtctttggg attttttaat attttatgtt taaatacagg
    22381 tacattaagt ttttcaccta gtttacggca ttgctcttta taggtacctt ttaatttatc
    22441 atgaaaaact atacaatcaa ttccaccttc aatactattt atacactttt ctagtttctc
    22501 aaatcccgat tcatcatcat acattccact tacaatccat tgatttggca atacatgtcc
    22561 aattgatatg ttatattctt tattttttat aatatctgag aaagatttct caaaatgtac
    22621 ccctccaaca cataataaag tcttcaaagg ttcatcacaa tcaaatactc taattaatga
    22681 ttttgccaat acctgtattg ctatactatt attaaaacta tcactactac ttcctatctc
    22741 aatatccaac ataggtacag aatattctgt aattaattta gattcttcac catatgttgt
    22801 tccagaccag tgagttgctt ctgttaatgt agtaaattca tcaagtagat tttcattttt
    22861 tatatcctca atagcattga ttagattttt aaagtatctt ggatttgaat ttccaaattc
    22921 tcctgttgga acatctccaa ttgtatgagc tgttaggata tgttctggag cattttctcc
    22981 ctcatgccaa tttacaactc ctgcaaaatc ataatctctg aaatgtctat ttaaaattgg
    23041 aagataatat ttataattat gacttattac atcttttgtt tttacaaaat gaaataagtt
    23101 attgttttta tccttaaatt ctaatacaaa attatcatca ataacaatat cagtttgatt
    23161 taaatccatt atttcttcac aacactttat cacattactg gctactgggt ctttctttaa
    23221 atcactacaa aagaaaaaaa ctgctttttt attattcatc cataaatcac actcctataa
    23281 cttaaatata atatatcttc atacctaaaa tcatctaaat atcactctaa ttccattaaa
    23341 ttatttatat ataatatact accatactca ttttagctat acctttgcct gagtttctat
    23401 atatatgtga tacatcagct ttaaatctta tagaatttcc actagataca atgtaatctt
    23461 ctccatttat tttaatggtt acttcgccac taaaaacagt tataaactct tgtgttcctg
    23521 gaagttcagc atttgaatct aaatttccac cttcttctat ttcaatcata aatatctcaa
    23581 atcttctttt ggagtcaaat ggaaatattg gataacttat atactttcca ttatcttcaa
    23641 taaaatggtc tatatcactt ttttgaatca attctatgtc actttcctgc ctctccatca
    23701 atgatgtaaa tgagattttt agtccattag ttattttctt caaagtagat atagtaggat
    23761 ttacctcaca cttttcaatt tgagatagca tactcttact tacaccacta agttgtgcaa
    23821 tcttgtctaa actaagatgt ttttctttcc ttatcttttt taaattttca gcgattattt
    23881 catttaaact ttgcataaat accccctaat acatactaat ataagataaa ttattttgat
    23941 tattaaataa acttagtaat ttaatagaaa ctttttatcc attatactgt acattatgtt
    24001 taatatacat tacttttttt agaatgtaaa tagttttctg aaattttgtg aaagtgaaca
    24061 aataaaaaac tagtaaccta tatttttaca aggctactag cttttattat aataatattt
    24121 cattttaact ataaaactta gatgcaccta taagctgtct cttagaaaca atcttagcta
    24181 ggtttgctaa tgaagaaatt tttatatctt taaaagaaac taaaaataca aacttaaaat
    24241 ctgtaaattc ttgatttata ttgtatatat caaaataaca tctcaaatta tcaaataagt
    24301 aattatcttg taagtctgct tctatctcat cagtagcatc ttttaaatac gctcttacat
    24361 cttcaaataa aacactgaaa taagactcat ctatttcttc acctaactct tccaatattt
    24421 cttctatatc taaatcatcc atatcaaaag aatttcctgt caactggcaa tcaaatacca
    24481 atatattttc ttgatttata gtatttgata tatcttgtac atattcgaag tctggacgta
    24541 attctaaaat gtcttttatt tcactgctct ttacatggat ttttcctttt ctattttcaa
    24601 tttttaaatc tatcatatgc tccccctttc aatctacaaa tctttaaata ccttgtttta
    24661 taatcttttc aatatctatc caattactaa ctctaactat attttgatgt ttaacatctt
    24721 tattatagtt agtatccaga agtattactt taacaccatc atcagctagt tgcactgagt
    24781 ttaaaggatt atcttctata aatatgtcac aactcagttc tcttgccttt tcaactttat
    24841 aatcacttcc taataagtaa acatctatat ctgaaaatcc ttgtctcgat agccaatttt
    24901 tagttacatc agttaaacac tcatgtctag ccgttacaaa agataaatta tgtcctttag
    24961 ataatgtgct aattacctct gtagcaccct ctacaatctt tgcttcatca tatgagtgta
    25021 tatacttatt attaaactcc gcatacacat ctcttattcc cgaaccatac aattcttccc
    25081 aattatgtgt tatacactct gcttcagtta tatttttatt aaagatatca tttaggtatg
    25141 gaagaaaatg ataagggctt gttattgttc catctatatc aatacatata ttcaatttgt
    25201 tcatttttac ctccatgtaa catttattga tttgattaat caaatattat agcacagctt
    25261 taattataaa tcaaaactct ggatatgact atacctatat aataccaaaa agcttgcata
    25321 tttaagcaag cttttcgtaa attttacatt tttaactaat atataagttt ttctaaggtt
    25381 ggttccactc caaattgtga tttcagttct ttatattctt ttaatgcttt gtcattattc
    25441 gttttagttt ttatcgctct tatcattaaa ttttttggtg tatcaagtgg agatatgtat
    25501 tctactacag aagtatcata tccattgccc tccaataaca atgttcttaa accatcagtt
    25561 atcaaatctg caaatctagc tttaaataca ccatgtttta atattggttc catagcttca
    25621 tatctatatt gccctaataa ctccttatga caacatggta ctactactat agcttcagat
    25681 tttgctctaa ttcctagacc tattgccata tctgtagctg tatcacaagc atgtagactt
    25741 attacaaggt caacatccct attaggtgta taattagtca aatcctcttt tataaatgac
    25801 atatttttat aacccaagtt tttagccata ttttttgatg cttctataac tacatctgaa
    25861 taatctattc ctataaaata acagtttttc ttcaatactt cttttatata aaaatttaag
    25921 acaaagctta agtaagattt tccacaagca caatccaaga tagttataca gtctttatcc
    25981 tttatttctt ttaaaatact atctatcaat tcaacaaaat ggtctatctg attgtatttt
    26041 cttattttat cattttttat cttaccatct tttgtaagta ctccaatttc ttttaaaagt
    26101 gcatttgctt gtcctacttt tacataataa tctctgtttt ttatttgaga tgccgtaaat
    26161 tcatctattt taggtacttc ttctttatta tctaaatatt ttacatttac cttcttattg
    26221 tctccttcaa ttacaacaat agttcctcgc tcaacatatt taaatactaa tccatcataa
    26281 ttttctgcct ctttagaaat attcttaagc aactcattaa acgtatataa atctgatttt
    26341 cctttaaaat taaatttata tttattatct tcctctattg caattccttt aaactctttt
    26401 cttccagatt taaaaatcac ctctaactca ataaaaaaat gcctattatc aaggtttctt
    26461 tgagtaatcc ccataaaaaa catatttagt tttcctatac tctgtttatt catcataatc
    26521 tcccttttaa cttatatctt taaatcttat taattaatat tatttgtttc tgaattattt
    26581 atttcttctt ttaaatcgtt caatctattt ttaaaacttt ctatctgctt atctgataaa
    26641 tcaagatatt ctgttatctt ccctatatat ttagggcttt tagtatttat taatttcaaa
    26701 gcactttcca tagatttttc tctaagctca tatgctttct ttacgtcatc tctagcatta
    26761 ctcaatgttt ctgtatactt ctccttagaa agttctggaa tctgcatatt ttcatattct
    26821 tttacaactt ctttacactt gtttctggca tttgtaacat ctttttttat actagataat
    26881 ttagttatat tttttgctat atccttagtg taaggtatac attctctcat tggaactaaa
    26941 gctgctcttt ctatattata aatttctttc ttaaaaattc ctatactttc catatctatt
    27001 ttttttatat cttcactatt tttttcttta cttgtatatt tattattttc ttttgtatta
    27061 tcttcttgtt tttctgattt attattcact gaaacattac tatcagaatt attcttttct
    27121 tttatatcat tacttccttc ttcattactt tctttatttt gtatatttct atcttgtttg
    27181 ttgtttaaat ttttattatt tgatatttct tcattctttt tattatcctg ttttaaatcg
    27241 gtactttctt tttccttaaa atttgataca tacttttcat catatgtctc atcagaaata
    27301 taattgtgat aatacataga ctcttttatt ttattaagtc cactcatata aatattactt
    27361 atcattattg ttgtagctaa taccacataa atactactgt agatacaaga cctcttaaaa
    27421 ttcttgtaat ttctgaatat cactactaaa ggaataagat ataaaaatat aaaaactaat
    27481 atccacctca taagtcgtgt ctcctttctc aaaaccgaaa tccctcaaat atttattttt
    27541 aatcatgtat cttatagtat ttaccaattt atgtttttta atcgtgaact tttattaaat
    27601 taaattctgt atagtcacaa gatgctaatg tatattctac tccatttcga atccccttca
    27661 atcccatttt aaaagattct ttaccatgca agtgaccata aatcaccttt tcaacattat
    27721 attcttcaaa taactttgta aataaagatt cttccaattt atcattcgta ggaggataat
    27781 gagtaatcac aattattttt gagtgaccac ttttacgagc agattctaat gatagtctaa
    27841 gtctatgctc ttctctttta taaacttttt catcagtctc atcaaattta acatcattag
    27901 gacatatcca gcccctacct ccacaaattg cataatcttt atattcataa aaatttgtct
    27961 gaataaaacg catgtcttca tatagcttat ttaaacttgt aacggttgtc caccagtaat
    28021 catgatttcc ttttataagt attttctgac ctggaagttt gcttattata tctaaatctt
    28081 ttttagcttc cgataaatta attccccaag atgtatctcc tagtactaag accatatctt
    28141 cttccttaac aacttctttc caattatcta taattttctt ttcatgacca tcccaattac
    28201 ttccaaagat attcattggc ttattgactg atgttgaaaa atgtaaatca cctatagcat
    28261 acaaactcat aatatcattc ctttcttaat tacactaaat aggctgtctc tttttagaaa
    28321 ttattttcca ttaagaggca gccccattaa tatttagcaa attttctaca agtagttttt
    28381 tactgaacta ctttatcatc taccttagta tccttatatt gctctagtaa tatatctatt
    28441 tcttgtttta attccaaaat ttcagttata tttttatctg tttcagttct atcaagtttt
    28501 ctttggtact gcatagcttt atgtgtatca cccaagtcat ataactcttg tattctattt
    28561 agagtatctc taatagctat agacttcatt tggtacatag cctgagcttt cattatgtcc
    28621 tctcttattt cactcatttc ttggtctaaa agaaaggcag catcagatgc tcttctaagc
    28681 ctttcagcaa gagtctcagg tatgtaatgg tcatatttat attttaatga atgttctatt
    28741 gtagcccaga aattcattgc aagagttctt atttgtattt cacatataat ttcctttgaa
    28801 cccgcaatgg agtttatagg gtattttatt ataacatgat agcttctata accactatct
    28861 ttgtaatttg taatataatc tttttctcct attatagtca tatcatttct tacttttatc
    28921 aaatcaacta tagcatatat atcctcaaca aactggcaca ttattcttat accagcaata
    28981 tcatcaattt cagtttctat atcttttgca tttatccttt ttaacttaga tattatggaa
    29041 gatatttttt ttgttctgcc tgtgacaaac tctatgggag aatattcacc ttttgctaaa
    29101 tattcttttc ttatattttt aaattttatt ttcaattcct ctactgcatg ttcataaggt
    29161 gctagtatct cattccattt ttcgtattcc atattgctca ccctttattt gttttttatg
    29221 accttcttta ttttatcata atttattttg tttttgtata gtaaagcaat tctccatttt
    29281 ccaatatata ccctatttct tctaaatcaa ctaagtaact tatgacagat actaacgaag
    29341 atttaaaaaa atgatactct ttataattat tgcttaaatt attgttattt ataatgtttt
    29401 ttaataaaat ttctaaagat ttcggttcta caagcaactc tcttatttga tataaatatt
    29461 tttttaaagc attcaaatgt ttttctatta atttataaga atcttctttc gatataactt
    29521 gcttgccatg ccctaaaact aagtactcaa aatctatatt ttttaatttt tctaatgaat
    29581 ttatttgttc ttgtatatca tatagaaaca agaaatcgta ttttgataga atttcatctc
    29641 ctatcaataa atcgccaaca aaaatcacat tatccttcgt caaaacagct atactaccag
    29701 gtgtatgacc ctgaaactca attatatcaa acatttcatt gtttaattca atttttccaa
    29761 cttctataga tttatctatc tttatatcat ccaacatttt attttttaag atattatcca
    29821 taaatacatt gctctttccc ccaacaatat actttgaaaa caattctgga ttttctatat
    29881 ataatttgtc gtattcagat gacattatac acacatcttt ataatattct ttaaattgat
    29941 tacaagctcc atagtggtca tcatgaccat gagtatttat aatatatcta acctgtatat
    30001 tattttcatc taatacttta tttactctcc taggtcttga accacataac cctgggtcta
    30061 tcataagtgc tgtgttatct tcaaatacat atattcctgt attcgtgcca ccttttataa
    30121 aaaaggtatt tccttttact tttgtaagat tcaccttcct cacctcattt tgacaatatt
    30181 tatgatacaa tttattactt actaatataa aactatacaa gaacaattct ttttattcta
    30241 tctatagttc tttttcttat tttgtattct ttagatatcc ttattttcac attatatgct
    30301 aaatttctat aaagtaatga ctctataaaa tattcaacta tacctctatc cctaaagtct
    30361 atatctatat ttccacttga ataaatattt gagtctaata tcataagctc atcaaaaaag
    30421 cttaatctct tagatttact taaataatct ttatttggaa taaatctatc acaataatct
    30481 atcctgtaat ttactacttc atcatttatt tcaaatccat attgcttttt cattaactct
    30541 ctatagttat tatttttctc aataacatta tctaaatttt tattataatc ctcttttttc
    30601 caatgtccaa acatcctaca atttaatggt ctaacttcat atattagaca tctattatta
    30661 gtatctttaa atggacatgg gttctttctt gtatactcct caaaataata atctaatatt
    30721 ttacttatac agcttttttt taaatcaact ctatcttcaa gataacaaaa tatattcaaa
    30781 aactcaatta aatttatacc tactgactcc atacaacaat ttccacatcc agaacaattt
    30841 cctactggca aggacttata tacatcattt agtttgtcaa agagcttatt tttaatggaa
    30901 taatctatac aatttaaaat atctttattt tttatactag tcattattat tactccttgt
    30961 tataattatt aagttaatac aaacaattat tataacatat tttttaaatt taatcgcata
    31021 tggaggtatt ttatgaatag atatacaaaa ataattaata tgatgggaag ttattacaca
    31081 aaagactttg aaaaagaaaa aaagaatgta ataaaagtta gagaagttaa agaagataca
    31141 gttagaaaat ttttcttgca aggtgactgt gaagttcttg tagtatttga agacactggg
    31201 aaagaaatac ttattgatga cttttctcct gaagaagata tcaaaaaata tttaggaaca
    31261 aaatttataa ataaaaaaag atagttatca aaaataattc taaaattaaa atttaataaa
    31321 tgtacagtta taattctata taattatagt taaaagttat cttttattgc ttttttagac
    31381 tttaagatag ctttttttct attttttgta aaataattcc ataattcaca ttaataattc
    31441 acaaaatgat ataatataac aaatagataa attttaaatt tctaaataca taattaatat
    31501 tttgcgaggt gattttatgc aaatttattt tagagttaag gctgttggca aacgcaaacc
    31561 tatgttagaa cttacccctt ttgatgtatc tgacaacatc aattgcctaa aagaagctat
    31621 ctctgaaatt gtatctaaaa atgttcttga atataatgag aaaatcccag aaaaatcaat
    31681 aataaacttc ttaactaatg aagaaattga atctcaagct gatattggaa aagttggatt
    31741 tggctctatt tacaatgaga ataaacaatc tgtagaaaag gcaatagaag tagcgttaca
    31801 ggcatttgaa gatggaatct acaaagtgct tataaatgaa gatgttattg aaaaactcga
    31861 ttcccctata aatctgaaaa gtggagatat atttactttt ataagactta ccttgcttgc
    31921 tggaagaatg ttttaaaata aataatttgg aggtacataa atgttagaac gtactagagt
    31981 agaaaaaaat cctaaaataa ttgaaaattt taaaaataat tttactaaat cttcttcaaa
    32041 acagtactac gacttagcaa aatctctttg ggactgcata gaaaatagtt catactatta
    32101 tagagaacct gaggacaaat tcttaaatat gataaagaaa tactacaatt gccactacta
    32161 cgaaattaaa aatatggacg agctcctttc tggaccttta aaaaatgcat tagaattaat
    32221 atttacttct gaacaagtag aagaactttg ccaattaatt aaaagaaaga tggattatac
    32281 atattcagga ggatattatc gtaaagggta tcgctcaaat aaacttgtgg attattttag
    32341 aggtattata aggtttattt caaactatgt tgatttaatt tattatgatt tttcagttat
    32401 agattatctt actcttccaa ataaggtctt gtactatgac tctataatga gtgataaaat
    32461 agcactagaa atagacaaag gaaatcaaaa agtaacaagt gccttagaag aagtaatatt
    32521 tggagaaaat caaactgctc tacttacaag acaaatgata attggtatca tgaagagtca
    32581 tagtgataaa tgctatgaaa tgctttctaa acttcttttg gcagccaaac aacaagaagg
    32641 acttagacag caaattgtag agagtttaga tgagggtaca atttctgcat caatatacat
    32701 gttaaaaact attttggaca acaatttaga gcgatttagt tctgttataa gagccttgga
    32761 tacatggaca ggacttggtt tttcagacca aaaacctgga ataatacgta aatgtttaga
    32821 actttcatat aaatcattga ctgatgaaaa gttccgtata gaatcaatta atagcaatga
    32881 taatcttgaa atttattttg ccttatgggc tactgcaact tacaacatat atgactctta
    32941 ctctcttatt gaaaacttac tttcttgtca ggaaaaatat aaaaaacttg tagctcttta
    33001 ctttttatcg catacagaaa gtacatattt taaacattct atagcatcta aaaacattaa
    33061 gggtgatgat ttagagatac tagcatgggt aattacaaat ttctatgtag aaggttctgt
    33121 tggctactac tatagttggc aaaacagaga aactaatttt gacttgtcaa acactcctat
    33181 tgacaaagac tttgatgcta gaaaagaaca atttgaaagt ttaaaacata ctttagaaat
    33241 tttaaattca aagacaaaat cattttcagg aagtgtattt ccatggactt ggattgaaat
    33301 ttcttctcat gaaatactta aatgtatgat gactttaaca gcatacgata tgaatgctca
    33361 atttatagat gtgcttatgg aaaattgtca tcttatgagt tcagatttaa gagcttcact
    33421 tatagtcaat tttgtaaatg aaccaaagac agatagacaa cgtgaatttt tattggaatg
    33481 tttaagtgat aagagtgttt ccaatagaga aacggtattg agtttactta gaaagcttga
    33541 gctaacagat gtagaattag ataagataac caatctattg tctttaaaga caagctctat
    33601 tagacaaaca gtaataaaaa ttttacttga acaagatgat tcaaatttgg aaaatattgc
    33661 ttcttcactg ataaaatctt caaattctca aaaatgcctt ggaggtcttg aaattgtaaa
    33721 cttattaaaa gacaattctg atagacaaga actatataat aagcttattt tacttataga
    33781 ggatattgat aaatctaaaa agaatacatc tcaagttcaa atattaattg ataatttgtt
    33841 aggtaaagca aaaaatgatt actgcaaaga aaatggtttt ggaatatata accctgaaaa
    33901 caaagttcaa attccagaac aatttgattt taaagatgat tctaatagct catcaaagct
    33961 taataaaatt atttcaaata taagtttatc tactagtgag gattctgttt ctgtagcaac
    34021 aaaaaaagaa gcaaaaaaaa ttctcgatac ggatataaat aaaataataa aagtttatga
    34081 aaaatttgat gagcttatta ctgaaaatgc agattatgaa tatgaagttg aaagatggtc
    34141 aggtaatgta gacaaagtgc ttttaggtta ttctacttat aacttgactc ctattaaata
    34201 taaagtttct aatgatactc ttgataatta tccattggca gaactttgga gagaaactag
    34261 taaaaagctt gacttagatt acaaattaat acttgatatc ttattttata gtataagtac
    34321 atgtggatat aatgaaacag aaaaaagttg gttttcatct atatattcaa atgtccttga
    34381 ctctaaaaaa caaaataaaa tatatacaaa acttgaaaaa tttaaattta atggacttac
    34441 aagaaaactt ttaagagttt tactaaatga gtttgaccat aaagaaatat ttaactattg
    34501 tttagaaata tactataaac ttagaaactt attgactata gaacaatgta aaaaacaaag
    34561 agttgaattt actaaaaact attacagaca aaatgaatac tgccctgctt taaatgcccc
    34621 tattttaaac tattggcttg ataaagcaca ttcgtttatt tatgatgata tttcttttac
    34681 taagtttttc tacactacgt tttcaaatta tcagttgcta gaatataagt ttcctttcta
    34741 tactagcata aatgtgtttg ctcgtgcaca tgaattaggt ttaatgactg aagagcaagt
    34801 taatagagaa atgataagtg gatacaacgc atcaaatata ataagaacat taacaaatcc
    34861 tagatgggct ggtgacacat tagaaaagta cccttggctt aaagataatt taaaaactgt
    34921 tacaaaccga attatagaaa ttgaatcacg tagaggtgat atgccaacag aagtgtctca
    34981 tctagctaat aatatacaat actttgaagg tatagattat tttgtaaata ttttggtcgc
    35041 tcttggtaaa gaaactttcc agagaggata ttcatatggt agcaattatt caaaaaaaga
    35101 tgttttaagt tctctactta aaaattgcta tcctactaaa gaagatgatt caaaaaaatt
    35161 aaaaagttta cttaaaaata ccgacattac tgagttaaga ttaatagaat ctgctatgta
    35221 tgcccctcaa tgggtaaaca taattgaaaa atatttaaac tggaagggtc tgaaaagtac
    35281 aatttggtac ttccatgcac atataaatga aggcttctct gcacaaaaag aatcagaggt
    35341 tgctctattc tcccctattt caccacgtag tttcattgat ggtgcatttg atgtagattg
    35401 gttcttagat tcatataaaa ctattgggga agaaagattc aatatacttt acaagtcagc
    35461 taaatatata actagtggtg gtaactctca tagacgtgca caactctatg ctgatgctac
    35521 acttggtaag cttgataaag acatgttgat gaaagaaatt gaaacaaata gaaatcaaga
    35581 taaattgaga agtttctctt tacttcctat gaaaaaagga gacctaaaag aagcaacttt
    35641 ccgttatgaa tttattcaaa agtttgctaa agaaagcaag caatttggtg cacaacgtcg
    35701 tgaaagtgaa tcaaaagctt gtactatagc gctagaaaat ctagctagaa gtgctggatt
    35761 tggagatgta aatcgtatga tgtgggctct tgagactgaa aaactaaatg aagttgctca
    35821 tctatttaaa ccaaaagata ttgatggtac ttcaatttgg atagatgttg atgaaagcgg
    35881 aaaagcatct actaaagttc aaaaaggcaa caagtcttta aagagtatac caaaagctat
    35941 tgctaaacat ccatatgtac ttgaacttaa atctgtccaa aaagatttaa agagtcagta
    36001 ttctcgtgca aaacagagtt tagaacaagc tatgaaaaat gaaatagtat ttacagttaa
    36061 agaaattgcc aatatctata aaaatccagt tatctgccct ttgattaaaa atctagtatg
    36121 ggtatgtgat gataatattg gatatgttta taatgataag tctaagttct ttatttgcaa
    36181 tatagaaggt gaaacttata agttgacagc aaaaaataaa ttaaaacttg cacatccagt
    36241 tcatttgttt gaatctggta agtggagtga gttccaacat gatttattta gtaagcaact
    36301 tgttcaacca tttaagcaag tattccgtga gtattataga ataaattctg atgaaatatc
    36361 tgaaaaaact atatctcgca gatatgctgg tcaccaaatt cagccaaaga aaactgtagc
    36421 acttcttaga aatcagcttt ggactgtcga ttatgaagag ggattacaga aagtatttta
    36481 taaagaaaat atagtggcaa agatgtttgc acttgcagat tggttttcac cagcagatat
    36541 agagcctcct actattgaaa cagtagaatt ttttgataga aaaacttata aaagtttaga
    36601 attagcttct atacctccaa ttctattctc agaagttatg cgtgatatag accttgttgt
    36661 aagtgtcgct catgctggtg gtgttgaccc tgaaactagc cattctactg tagagatgcg
    36721 tatagcaatt gctaaagaac ttttattatt attaaaaatt aacaatatca attttgaagg
    36781 ctctcatgtt cttatcacag gtaatttagg tgagtattct gtgcatatgg gttctggtat
    36841 tgtacataaa aaagggatgg gtgctttgaa tatacttcct gtacattctc aaagtagagg
    36901 taagatattc cttccattcg ctgatgaaga ccctaagact gctgagatta tgtctaaaat
    36961 cttattactt gctgaagata ataagattaa agaccctagt atcttaagca acttataaat
    37021 ataagattta tctctaaaac atattgggct ttctaatttt ccatgaaagt ccaatatgtt
    37081 ttatattata
//
LOCUS       DAEPLF010000019        35625 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEPLF010000019 DAEPLF010000000
VERSION     DAEPLF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35625
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.019"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            437..1459
                     /locus_tag="KR314_000829"
     CDS             437..1459
                     /locus_tag="KR314_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH0327228.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            1755..2675
                     /gene="pyrB"
                     /locus_tag="KR314_000830"
     CDS             1755..2675
                     /gene="pyrB"
                     /locus_tag="KR314_000830"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBH0327229.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            2714..3406
                     /locus_tag="KR314_000831"
     CDS             2714..3406
                     /locus_tag="KR314_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBH0327230.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            3399..4301
                     /locus_tag="KR314_000832"
     CDS             3399..4301
                     /locus_tag="KR314_000832"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBH0327231.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            4386..4970
                     /locus_tag="KR314_000833"
     CDS             4386..4970
                     /locus_tag="KR314_000833"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBH0327232.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            5185..6111
                     /locus_tag="KR314_000834"
     CDS             5185..6111
                     /locus_tag="KR314_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HBH0327233.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFNYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIGKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYILSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVENSLHRILEHSKEGRTSMLQDVEAHRPTEVDMF
                     SKNICKLGKKYSIPTPINQTLFYMIKVIESRF"
     gene            complement(6163..7053)
                     /locus_tag="KR314_000835"
     CDS             complement(6163..7053)
                     /locus_tag="KR314_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0327234.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSQKYPDIYVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYNSHLLKDFSFDKLQDQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            7241..8272
                     /locus_tag="KR314_000836"
     CDS             7241..8272
                     /locus_tag="KR314_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBH0327235.1"
                     /translation="MLKKKSSLTLKTIREILPGLFISVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGAIVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            8566..9441
                     /locus_tag="KR314_000837"
     CDS             8566..9441
                     /locus_tag="KR314_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HBH0327236.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     RGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            9459..10949
                     /gene="cls"
                     /locus_tag="KR314_000838"
     CDS             9459..10949
                     /gene="cls"
                     /locus_tag="KR314_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBH0327237.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            11142..11426
                     /locus_tag="KR314_000839"
     CDS             11142..11426
                     /locus_tag="KR314_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBH0327238.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            11459..13087
                     /gene="groL"
                     /locus_tag="KR314_000840"
     CDS             11459..13087
                     /gene="groL"
                     /locus_tag="KR314_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBH0327239.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVIVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            13349..14089
                     /gene="larB"
                     /locus_tag="KR314_000841"
     CDS             13349..14089
                     /gene="larB"
                     /locus_tag="KR314_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBH0327240.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            14173..14907
                     /gene="larC"
                     /locus_tag="KR314_000842"
     CDS             14173..14907
                     /gene="larC"
                     /locus_tag="KR314_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBH0327241.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            14937..15356
                     /locus_tag="KR314_000843"
     CDS             14937..15356
                     /locus_tag="KR314_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBH0327242.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKVTP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      15416..15517
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15738..17273
                     /gene="guaA"
                     /locus_tag="KR314_000844"
     CDS             15738..17273
                     /gene="guaA"
                     /locus_tag="KR314_000844"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBH0327243.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTSITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            17812..19242
                     /locus_tag="KR314_000845"
     CDS             17812..19242
                     /locus_tag="KR314_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0327244.1"
                     /translation="MILNQIFIEDGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGEYISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKNNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(19390..20265)
                     /locus_tag="KR314_000846"
     CDS             complement(19390..20265)
                     /locus_tag="KR314_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0327245.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETSLFDRLPRGVCLTRCGISLLDEARSILTACRNLDKKISRLEECTPINIVSSITIAS
                     FLLPPILSRLKNSFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACAPTFPLSEQTLSLRQLCTFPLLLREQGSAIRDTLDSVLSLSNQKAYPVWE
                     SVNSFALIKAAEAGLGITILPENLLLDSLLHKKLRLIELDEIDMENKMLAILHKDKNI
                     TQPLEIILDNISNVL"
     gene            20385..20948
                     /locus_tag="KR314_000847"
     CDS             20385..20948
                     /locus_tag="KR314_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH0327246.1"
                     /translation="MNQSRKIYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKQFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCTAAIIPPLVILGLVSAF
                     YTTFISNAIVTAVLKGMQAGVAALIVDLIIDMCSMILKQRSLFLSTMIPIAFFANFVM
                     SINVIFILSVCCFVCVMQVFYKKRENK"
     gene            20945..21511
                     /locus_tag="KR314_000848"
     CDS             20945..21511
                     /locus_tag="KR314_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH0327247.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     ILLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(21640..21876)
                     /locus_tag="KR314_000849"
     CDS             complement(21640..21876)
                     /locus_tag="KR314_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327248.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(22186..25614)
                     /gene="uvrA"
                     /locus_tag="KR314_000850"
     CDS             complement(22186..25614)
                     /gene="uvrA"
                     /locus_tag="KR314_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0327249.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFENELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFAKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLGNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKTPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPNAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPNKNYTALTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFTETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCNEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            25933..28167
                     /locus_tag="KR314_000851"
     CDS             25933..28167
                     /locus_tag="KR314_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0327250.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMDEILNIDSFNGEGTIEIINSQGKTLFIGKNNELIKDLSYKYKDSADEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDVVLGELNK
                     LNSFTIIMTFITTCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSNFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            28423..30453
                     /locus_tag="KR314_000852"
     CDS             28423..30453
                     /locus_tag="KR314_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0327251.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDLIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENEIILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDEDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            30450..30902
                     /locus_tag="KR314_000853"
     CDS             30450..30902
                     /locus_tag="KR314_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0327252.1"
                     /translation="MNKLVNVKCSFKIHANSKEEAIVSLVDVVSKEGYLIDKNQFLED
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDIPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            30904..31959
                     /locus_tag="KR314_000854"
     CDS             30904..31959
                     /locus_tag="KR314_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBH0327253.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            32026..32334
                     /locus_tag="KR314_000855"
     CDS             32026..32334
                     /locus_tag="KR314_000855"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBH0327254.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            32438..33703
                     /locus_tag="KR314_000856"
     CDS             32438..33703
                     /locus_tag="KR314_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBH0327255.1"
                     /translation="MKKLPIKTVVQRLLDLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKIADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLSVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVDKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKNEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            33791..34507
                     /locus_tag="KR314_000857"
     CDS             33791..34507
                     /locus_tag="KR314_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBH0327256.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(34702..34995)
                     /locus_tag="KR314_000858"
     CDS             complement(34702..34995)
                     /locus_tag="KR314_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBH0327257.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(35001..35216)
                     /locus_tag="KR314_000859"
     CDS             complement(35001..35216)
                     /locus_tag="KR314_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327258.1"
                     /translation="MRTLSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
BASE COUNT        13565 a         3938 c         6252 g        11870 t
ORIGIN      
        1 ttaacttaga attattagaa tttgagaatc actattagga aataggtttt gatttatgca
       61 gggttaaaaa tttcacagaa tatctaactt tatactaaat ccatcaaaaa atatacgcca
      121 tataaaagcg cataaaaaaa cacataaaac aatatttatg cgcactttaa cctcgaatgg
      181 tacaaaattc tttactttta cagacccata ttatataatt aagtaaataa tgtatattta
      241 ggtgacatat tggataagct aatttattta ttttattaat ataatgatac tataaaagtt
      301 atataaagtg gtttacaaaa aagttacaga tagagctttt attaatgtat tgtgtattag
      361 tattttatcc ttaggtacac ttcacctaca aaatagaatc ataagataat tatgggaaaa
      421 ttttaggagg tttatcgtga agaaaaagat attaatacca gtatttgcat cagttatggc
      481 attatcagta agttcaattg taaatgcaga tgaagtaaat gattcatctc aaaataagga
      541 tgataaaaca aatactgaat taaatttagg tgaatataaa gaagtaaaat ataaagttgc
      601 aaaaataaaa gatggagtag ctataaagat aagagaagaa ggtcaagttc aaaacatagc
      661 ttattctgga gatgaattta cggttttagg aactcaagga gaatgggtta aggttaaagt
      721 tgaagatggc gaaggttggt tagctactag atatgtagat atatctgaag gtgtaggata
      781 tactaacgct gataaagtaa acttaagaaa agataagagt gaaagttcag aagtaattga
      841 agagctggaa aaaggaagtt ctttattggt tttggaggat aatggagatt ggttaaaagt
      901 taaagatggg gaaacagaag gatatgtaaa atcatcttat atatctgata aagctcctgt
      961 aatagaagag ccacaagtaa acccaagtat tgaccaaaat gtagattcta caaataataa
     1021 tcaacaaaac aatgcaaata ctaatcaaca aaataataat tcaaatgtgc caacagctaa
     1081 ctctaatgca gttcaagctg tacttaactt ggcatattct aagcaaggtt gtccatatgt
     1141 ttggggagca gaaggaccaa acacttttga ctgttctgga tttacacaat atgtgtatag
     1201 aaatgcagta ggaaagaata taccaagaac atctaaagca caatctaaat atggacaaac
     1261 tgtaagtaaa gcaaatttac aaccaggaga cttagtattc ttcacaacta atggttctgg
     1321 ttctgttagt catgttggaa tatatgtagg tgggggtaat atgatacatt caccatcaac
     1381 aggaaaaaca gttagtgtaa ctagtataaa ttcatcatac tatacagcaa gatttgttac
     1441 agcaaaaaga atattataat cggtttgtaa tatgacaaat tgataaatac ataaatatat
     1501 ataaacgctc tatattatat ttaatttaat catggaaaca aaaaatgatt atattttaaa
     1561 tgtactatag agcgtttttg tatcacttta aatctaatca agattcaagt taatatgact
     1621 attaaatagg agccttttca tatgatgatt atttgcgcaa tcccttgaca ggatagggta
     1681 aaggtggtaa gctactaaca gtacaaaaat acttttaatt ttagttcaga gaggctaaaa
     1741 aaggaggacg aaagatgtta aaatcaagaa atttaatcca accagaagac ttttcaatag
     1801 aagaaataga tgaaatatta gaattagctc aaaaaataat tgacaatccg tcaaaatatt
     1861 ctcgtatatg tgaaggaaaa ctattagcta cattatttta tgaaccatca acaagaacaa
     1921 gattaagctt tgaatctgct atgaacagat taggaggacg tgtagtaggc ttttcagagc
     1981 caaattcatc ctctgcatca aaaggtgaaa ctctaggaga tacaatgagg atagtatctg
     2041 gttatgttga tataatagct atgagacatc ctcaatcagg tgcagcttca gaagcagcta
     2101 gatatacgga agtgccattt ataaatgcag gagacggcaa aaaccagcat ccaactcaaa
     2161 cacttacaga cttacttact ataaaatcat taaaaggaac tttagaaagc catacaatag
     2221 gattatgtgg tgatttaaaa tatggaagaa ctgtgcattc tttggttaag gctatggcaa
     2281 gatataaaaa tacaaagttt gtatttatag cgcctgaaga gttaaaaatg ccagattata
     2341 taaaagaagc tataaaggga catgcatatt atgaaactaa taatttggat gatgttatag
     2401 gaagcttgga tgttttatat atgactagag ttcaacaaga aaggtttgaa gataaatcag
     2461 agtatgaaag acttaaaaac tattatattt taaataaagc taaattagaa aaggcatcta
     2521 aagatatgtt agtaatgcat ccattaccaa gagtaaatga aatagacata gatgtagata
     2581 gtgatgatag agctgtatat tttaaacaag ctaaatatgg aatgtatgtt agaatggctt
     2641 taataataaa acttttaggt ataaatgaag actaatagtt atcatataat agtaaaacaa
     2701 aggagatttt gggatgtata aaattctgga aaatatatat ataggcgaag atatgtatag
     2761 aatgaaagta aaaggtaatt ttgaaggtaa aatgggacaa ttctatatgt taagagcatg
     2821 ggatacttat ccagtacttt caagacctat aagtatacat gatatagatg aagaagggat
     2881 aacttttctt tataaagtag taggagaagg aactcaaatt ttgagtaacc taaaggtaaa
     2941 tgataatata aaattagaag gcccttatgg taatggatat gctaaggttg atggaaaggt
     3001 agctctagta ggaggaggaa tcggtgtagc tccactatat ttggttgcta aaaatataaa
     3061 aaattgtgat gcatatcttg ggtttagaga agatgttata ttagaagatg aatataagca
     3121 agtatgtaat aaagtctata caacagtagg aaatactttt gtaacagata taattgatgt
     3181 tgagaaatat gattatatat taacatgtgg tccaactcca atgatggaaa aattagttaa
     3241 gatggtagaa ggtactaaaa cgagaattat ggtatctctt gaaaaccata tggcttgtgg
     3301 agtaggtgcc tgtttagtat gtacgtgtaa aactaatgga ggaaacaaaa agacttgtaa
     3361 agatggtcca gtgttctggg gagaggacgt gatttttaat gggtaattta agtgtgaaat
     3421 ttggaagtgt agaatttaaa aatccagtaa taatggcgtc tggaactttt ggatttggaa
     3481 aagaatataa tgagatttat gatatacaaa aacttggtgg tataagtagc aagggtctta
     3541 ctttaaataa aaaacctggt aacaatggta tgagagttca tgagacacca tccggtatga
     3601 tgaatagtgt tgggcttgaa aaccctggag ttcaaggatt tatagactat gaattacctt
     3661 tttttagtaa actagactta gtcagaatag ctaatgttgg aggtgggaca ttagaagact
     3721 acttattagg ggttcaaatg ttaaatgaca agcctataga cattattgaa ttaaatatat
     3781 cttgcccaaa tgtcaaagct ggtggaatgg cttttggtat aaaaaatgaa gtggcaagag
     3841 aagttgttcg tgaggtaaga aacataacga aactgccttt agtaataaaa ttatcaccca
     3901 atgcagaaga tattgtagga atggcaaaag tttgtgaaga agaaggtgct gatggagttt
     3961 cattagttaa tacttttaaa gcaatggcaa ttgatataaa aaatagaaga ccagtatttg
     4021 aaaatgtata tgctggatta tctggaccag ctataaaacc tatagctttg agaatggtac
     4081 atgaagtgtg taaaaatgta aatatacctg taatgggaat gggcggaata actaaagcca
     4141 cagatgctat agagtttata atggcaggag ctacttgtat ccaagttgga actgcaaatt
     4201 ttatgaatcc tagaataggt attgaaataa tagatgggat taatgaattt atggatagag
     4261 aaggaataaa aagtttagac gaaataagag gaattatata aggtagaata aatagtattt
     4321 taaagataaa aaatatagat taaataaaca ataattttat aacattaaca tggaggaaaa
     4381 ataatatgag taaagtagat gtagtggata tattaaagaa aagtgatgca ttgttggagg
     4441 gacatttttt attatcttca ggaaaacaca gtaatagata cgtacaatgt gcaaaagtat
     4501 taagatttcc agaatatgct gctgaggtat taagtacagt tgttgaacaa ataaaagact
     4561 tagatataga cttagtagta ggaccagcta tgggtggagt aatagtttct tatgagttag
     4621 gaagacaatt aggaaaagaa gctgtattta ctgagagaaa agacaataca atggagttaa
     4681 gaagaggatt tgaagttaaa aaaggagcaa agataataat tgctgaagac gttgtaacta
     4741 ctggtaaatc aactatggag acaaaaagag tattagaagc cttaggtgga gaagttgtag
     4801 gtgttgcatg tatagcagat agaactaatc atgatatagg tatgcctata tatagtgcta
     4861 taaaacttga tattcaagtt tatgaatctg atgagtgtcc tttatgtaag gaaggaaaat
     4921 taccagttgt taagcctgga agtagagagt tcaaggaatt agggatgtaa taaaaactta
     4981 attatttata gtgttactta aaaaatgtaa attttttaga gtaaaaattt gtaatataac
     5041 atataactta aaaataaaaa gcagttagcc ttataaatga aggttagctg ttttttttgt
     5101 taaaaatata tttacaaaat aagagatagt agcagtacaa tataagtata aggtaaaagt
     5161 attatatatt agggaggggt aagtatgaaa aatcaattaa atgaaaatca taaaaaggtt
     5221 gtattttttg gagtaggtgc tgtaggagct acttttgcag aacaattttt taactctaaa
     5281 tatgatttta aaattctttg tgataatgaa agaaagaaaa gatatttaga agaaggattt
     5341 ataataaatg gaaaaaggta tgattttaat tatgtaacta aagatgagta taaacaagag
     5401 gctgatttta taattatagg tctaaaatat aataatttaa aagaaaatat aaaagaatta
     5461 gatggattag ttggaaaaaa tacagttata atgtctctgc taaatggagt tgatagcgaa
     5521 gagataatag gagaaagatt tggaattgga aaaatggtat attcatatgt taccaatata
     5581 gatgcaaaga aaatcaataa taatattata cacactacta atgggataat tgtatttggt
     5641 aacaaagata atagtgaaga tagaaaaact aatataataa ctgaagtctt tgatgatgta
     5701 aatatagaat atattttatc aaaagatatt caacgagata tgtggtggaa gtacatggtt
     5761 aatattggtg taaatcaaac ttcagctata cttggtgcac cttatggagt ttttcagagt
     5821 tctgagcatt taagagaatt agcaaaatct gcaatgaggg aagttgttgc tatagcacaa
     5881 gcaaaagaca tatctcttac agaagatgat gttgaaaatt cattacatag aatactagaa
     5941 cattcaaaag aaggaagaac atcaatgctt caagacgtgg aagctcatag acctacagaa
     6001 gtagatatgt tttctaagaa tatctgcaaa cttggaaaaa aatatagcat acctactcct
     6061 ataaatcaga ctttatttta tatgataaaa gtaattgaaa gtagatttta aagggtattt
     6121 gtaagaaata ttgataatta ttgattttaa attttattaa tgttatatac tatttaagtt
     6181 actaaatatt tttaactctt caatgaaaat agttgctgct tttgacaaag ttatatcttt
     6241 tggaaatata tatgagaaat gtctgttgta gctgcttcca agctctatga cagacagctc
     6301 attgtttaaa acaggtaagc ttgtcactag attagaaact attgtaattc ctaaattatt
     6361 tctaacagat tctttgacag cataattact tccaaaaacc atcatacttt ttggaataat
     6421 ttctttaaca cttaaaaaca tatctaaata gtctctggtt ccagaaccat cctccctgac
     6481 tatccatttt tggtcttgaa gtttatcaaa actaaaatct tttaataaat gactattgta
     6541 tggaagtgct aaaaccatct tatcttcaaa aaaatattct tgaataaatg aaggggaaga
     6601 gcaagtgcct tcaataagac caatgtctaa aatataatcc ttaacatgag aagatacgat
     6661 tgatgtattt tttataaaaa cttctacata tatgtcagga tacttttgag aaaataatgc
     6721 taaaaaattt ggtagaatat attctccaat agttaaactt gcaccaatct ttaagtgacc
     6781 agttatagca tctgaaactt ggcttacatc atgataagta atatttaaaa gatttaatat
     6841 ttctttagct cttttgtaca atatttgtcc actttctgtt ataaaaatgg ttttttgttt
     6901 aatagacctg tttataagca caactttaaa ataattctct agatttttta tgtgtttact
     6961 tacacttggt tgtgatagat ttaagtattc cccagcttta gtaaagtttt tgtattcaac
     7021 aacagctaca aatgttttta attcttcaaa caattttttg tctccttcac ctaatgaaat
     7081 tttacatttt ttatatactt tctacagata attatactat atataataac cattaaaaac
     7141 attaatcatt acgatattat atatttattt tacttatggt tattacaatt atatactatt
     7201 gctatgaaga aaactatatt aattataagg taggtatatt atgttaaaga aaaaaagcag
     7261 tttgacatta aaaactataa gggaaatatt accaggactg tttatatcag tgttagtagg
     7321 ttatattagt atatttattt ctattttaat tcctaaggta ggggcagcat caatttctat
     7381 atttttaggt atgtttgtag gaaacctatt tttaaatcaa aaggtatttc aaaaaggtta
     7441 taaattttca gagacagatt tgttatcata ttcaatagta ctattagggg caacactcag
     7501 tgtatctacc ttaatagact taaaagtttc aggaatttta tttataatat tgcaaatgac
     7561 aataacaata atagctgcac tttatatagg taaaaaacta ggatttgaag aaaactttag
     7621 atttctaatg gcaagtggaa atgcagtatg tggttcttct gccataggag caatagttcc
     7681 agtggttaat gcgacagata aagaaaaggg aattgcagta actattgtaa atgttactgg
     7741 tatattcctt atgtttttac ttccaataat atctcaattt ttatatagcc atgaacttgt
     7801 aaaaacttca gcaatgatag gtggaacttt gcaatcaatt ggtcaggttg tagcaagtgg
     7861 agcaattgtt ggagaaaatg taaaagattt ggctactatt tttaagatag taagagtaat
     7921 atttctagtt gttgttgtat tagtatttgg acatatgaaa aatcagtcta atagggaaat
     7981 ccttgaagaa gaaaaggatg agataaaaaa gaaaaaagta actataccat ggtatgtaat
     8041 tggattcttt ataacttgtg cacttttttc aatgaacatt ataccaaaag aagtatctgt
     8101 gttgtgcaaa gaaataagta acaaactaga aataatagct ttagcagcaa ttggattaaa
     8161 agttaatgta aaagatttag tgaaacaagg gaaagaagtt tctttatatg gtttatttgt
     8221 aggtacagta caagttgtat cagctgtagt tttaataaaa atatttatat agcacaaaaa
     8281 tagtatttgt aatattaatt tgattaatta aaataatatg atactaataa tttataatat
     8341 aaataaaaaa tgaatttcga taagtttaag tgggtataaa cttatagaaa ttcatttttt
     8401 atttaaggta aaaatttaaa gtaaaatatt attttacata ataataacct tataaatttt
     8461 gaaattactt atctttgtgt taaaataagt aaaatacaca tattaaagtt aagtttttag
     8521 gcaaataaac aaaatgaaaa tataaatatt aaggagattt ggtatatgaa tgtttatgaa
     8581 aaaggtaatg gagttatact agaaggagta acagattttg acccagtaca tatatttgag
     8641 tgtggtcaat gttttagatg gcacaaacaa gaagatggct catacactgg agttgcaagg
     8701 gggagaatat taaatgtcaa aaaagagaat gataaaatat atttaaataa tacgaattta
     8761 aaggagttta acagcatttg gtataattac tttgatttag gaactgatta tacagaaata
     8821 aagaataagc taaaaaatat ggatgaatat ttgaataagg ctactgaatt tggatgggga
     8881 ataagaatat taagacaaga tggatgggaa atgcttatat catttataat ttcctctaat
     8941 aatagaattc ctatgataca aagagcgata gaaaatttat caagaaagtt tggtaagtat
     9001 ataggtgaat atgaaggtaa tgaatactat gcattcccaa caccagaaga actaaataag
     9061 gcctcacaag aggaaataag agcatgtcaa acaggtttta gagataagta cataaaaagt
     9121 actacacagg cagttataga gaataatgat gaggtatctg aatatacaaa tttaagtaca
     9181 gaggattgta gaaaagagct tttaaaattt aatggagtag gaccaaaagt atgtgattgt
     9241 attgcattat ttggtatgca aaaatatgat tcttttccag ttgatgtttg ggttaagaga
     9301 gttatgcaag aattttatat agatgaagac atgagtttac ctaaaatgag aacttatgga
     9361 atcgataaat ttaaagaaat gtctggattt gctcaacagt acttatttta ctatgcaaga
     9421 gaactcggta taggaaaata aattaagggg gagaaattat gggagtaata ggaacaatat
     9481 ttctgttcta tttaattata tcatatctag ctggagccat aatttctgtg attatattgc
     9541 ttgaaaatag agaccctgca aaaacaatgt cttggctatt gatgtttatt atatttccag
     9601 gtgtaggttt aatgatatat gctatttcag gtagaaatat tagaaaaaga aaattattta
     9661 agactcaaaa actagctaat aatataaaag aaaagaaact atttgataca ttagaaaaga
     9721 taacagaaat agttgaatta gaaaaagaat ctataaaaca aaataagctt ttaagagatg
     9781 aagaggatgg tagttataga aaaagggtaa taaatatgtt gttaaagact ggtatgtttc
     9841 cctttactaa aaataataaa gtggatgtat ttgtagatgg aaatgaaaag tttaaaagac
     9901 ttatagaaga tataagagaa gctaaagacc atattcattt agagtacttt atcatcaaag
     9961 atagtgaaat aggtagggtg ttaaaagaag aacttataaa aaaggctaaa gaaggcatta
    10021 agataagaat tttatatgat gatgtaggat gctggagatt ttggtttaat agaaaatttt
    10081 tccgagaaat gagggaagta ggtatagaaa ttgcagcatt tttacctaca aaatttccta
    10141 taataggagg gaaattaaac tatagaaatc acagaaaaat tgttgttatt gatggaataa
    10201 ttggatatac aggtggaata aatataggag atgaatattt aggcaaaaat gataagtttg
    10261 gatattggag agatactcat attaggatta agggtatttc tgtatacatg cttcaaatga
    10321 catttctgat tgattggtat tatacgacaa aagaagtatt agtgactaaa aattatttcc
    10381 caagtgtagg gaatgttggt gaaagtatga tacaagttgt tgcgagtggt ccagacagtg
    10441 actgggaaga tattcattat gcttattttt ctgcaatatg tcaggctaga aaaaatgttt
    10501 atatagaaac tccatatttt attccagatg aaagtctact aaaagctata aagagtgctg
    10561 ctcttagtgg agttgatgtg agaataatat ttcctaaaat tgcagaccac aagatagtaa
    10621 atatagcatc ttattcatat tttgaagaaa tattaagagc aggtggtaaa gtttatttat
    10681 ataataaagg atttatacat tctaaagtcg taataataga tgataaaatt gcttctgcag
    10741 gtacagctaa tatggattta agaagtttta tgcttaactt tgaggtaaat gcatttatct
    10801 atgatgaaga agttattagg gtaatgacag atgatttctt tgaggattta agtcactgtg
    10861 aagaacttaa tttggaggta tttaagaata gaaatatcat acaaaaaatc aaagaatctg
    10921 tagcaagatt attttcacca atattataaa aaattattaa aaaaggtatt gaaaattatg
    10981 tgtgattggt atattataat aattgttagc actctattag atagagtgat aataaataaa
    11041 aagtatatat ataaatctac atatataagt agtattaatt taaaaataac taaaaacata
    11101 ggcgaactag tctatactta atattttagg aggcgtgaat aatgaaaata agaccattag
    11161 ctgacagagt agtaattaaa aaagtagaag cagaagaaaa aactgcaagt ggaatagttt
    11221 taccaggagc agctaaagag caacctcaaa tagctgaagt tgtagaagtt ggaccaggtg
    11281 gaatagttga agggaaagaa ataaaaatgg aattgacagt gggagataag gttatattcc
    11341 aaaaatattc tggaacagaa gttaagatag aaggacaaga atacacaata ctaagacaga
    11401 gtgatgtatt agctgttatt gaataaatat agaataaatt tattaggagg gatttaaaat
    11461 ggctaaagaa attaaatttt cagaggaaac aagaagagct ttagaagctg gtgtaaataa
    11521 attagcagat acagtaaaag taacattagg acctaaagga agaaatgtta tattagataa
    11581 aaaatttggt tctccactta taactaatga tggagtaact atagcaaaag agatagaact
    11641 agaagataga tttgaaaata tgggtgctca attagttaaa gaagtagcaa ctaaaactaa
    11701 tgatgtagct ggagatggta ctacaactgc tacagtttta gcacaagcta taataagaga
    11761 aggtttaaag aatgtaacag caggggctaa cccaatactt ttaagaaaag gaatacaaaa
    11821 agcagtaata gtagcggtag aagaattaaa aaatcaatca agaatagtag aaacacaaga
    11881 ggctatatct caagttgctt ctatatctgc tggtgatgaa gaagttggaa aattaatagc
    11941 agaagctatg gaaatagtag gtaaagatgg agttataact gttgaagagt ctcaaactat
    12001 gaatactgaa ttagatgctg ttgaaggtat gcagtttgat agaggatttg tttctgcata
    12061 tatggttaca gatgtagata aaatggaagc agttttaaat gacccatata tattaattac
    12121 tgataagaaa atatctaaca tacaagaatt attaccagtt cttgaacaaa tagttcaaca
    12181 aggtaaaaag ttattgataa tagctgaaga tgtagaaggt gaagcattat ctacattagt
    12241 agtaaataaa ttaagaggaa catttgatgt agtagcagtt aaagctccag gctttggaga
    12301 tagaagaaaa gaaatgcttc aagatatagc aatacttaca ggtgctcaag taatatcaga
    12361 agaattaggt tatgatttaa aagaagctga tttatctatg ctaggtagag cttcatctgt
    12421 taaggtaact aaagaaagta ctacaatagt agatggttct ggagataaaa aagctataga
    12481 agatagagta actcaaatta aacatcaagt agagcaaact acttcagatt ttgatagaga
    12541 gaaattaatg gaaagattag ctaaacttgc tggaggagta gctgttgtaa aagttggagc
    12601 tgctacagaa gtagagttaa aagaaagaaa attaagaata gaagatgctc ttaatgcaac
    12661 tagagcagct gtagaagaag gaatagtagc tggtggagga actgcttttg ttagtgttat
    12721 accagcaata ggaacactaa ttgagagttt agaaggagaa gttaaactag gtgctcaaat
    12781 agttaaaaaa gcgttagaag agccattaag acaaatagct ataaatgctg gtcttgaagg
    12841 tgctgtaatt gtgcaaaatg ttgttaattc tgaagcagaa actggatttg acgcattgaa
    12901 tgaaaaatat gtaaatatga tagaagctgg tatagttgac ccaactaaag ttagtagaag
    12961 tgctttacaa aatgctgcat caatagcaag tactttctta actactgaag ctgctgttgc
    13021 tgaccttcct gaaaaagaag atgcaggaat gccaggaatg ggtggcggaa tgcctggtat
    13081 gatgtaattt aaaaaattta aaatataaat tttaatataa agaactattt cgacttagat
    13141 tttaaactct aagttgaaat agttcttttg tatatatcta atttttgtat atctataaat
    13201 tcaatacaca tatttagata atcagtatct agtttgtaat aatattttag agtagtattg
    13261 ttatttttta ggttatgatt ttagatatat attaattaag gtataattgt tgatagagaa
    13321 taaatcaaat ttaaaaggat gcgataatat ggacttaaga aaattacttg aagaagttaa
    13381 aaatgaaaat attgatatag attttgcgtt agaaaagtta aaagatttac cttatgaaga
    13441 ccttggatat gcaaatatag accatcatag agaattaaga aatgggtatc cggaagttat
    13501 ttattgtgaa ggtaagagtg atgagcatat tttagggatt attgataaaa tgaatcaaaa
    13561 aagttctaac atattgggga caaggtgcag aaaagaaact tttctgaaaa ttaaagaaat
    13621 ctataatcat gctgagtacg aagaggcgtc taaaattctt aagattcaaa atcatgatat
    13681 agagaatata ggaaaaggta aaattgtgat tgctacaggg ggaacttctg atataccagt
    13741 agcagacgaa gcttatcata cagctaagtt tttaggtaat gatgttgata gaatatatga
    13801 tgttggagtg gcaggtatac ataggctttt aaataaaaga cataaaatag atagtgctag
    13861 agtaatagta gccgttgcag gtatggaagg tgctcttgca agtgtaattg gtggactggt
    13921 tgatgttcca gtaatagctg ttccaacttc tgttggatat ggagcaaatt ttggaggatt
    13981 agcagcatta ctagctatgt taaatagctg tgcatctgga atatctgttg taaatattga
    14041 taatggattt ggagcaggat atttagcatc aatgataaat aaactgtaaa tacattgata
    14101 taaattaaac aaaactatat aataaaattt aatttctatt atatttttac tattaaaatt
    14161 gaggagacat atatgagtga aaaaatactt tattttgata taataagcgg tatatcaggg
    14221 gatatgaccc ttgcaagttt attaaattta ggtgtgccta aggaaatttt tttggaggaa
    14281 attcataaac taagaatgag cgatgaattt aatataaaca tcagctcgaa aactgaaaat
    14341 ggaatagtag gtacaaaagt agaagtaatt acaaaagaaa aacatacaca tagaaattta
    14401 gtggatatat ttgaggttat agataaaagc actctaaacg aaaatgtaaa atctaaagca
    14461 aaaaaaatat ttatggtaat tggagaagca gaagcaaaag ttcatggaac tacaatagac
    14521 aagatacatt ttcatgaggt aggggctata gattcaattg tagatatagt aggagcttct
    14581 atattagttg acttactttg tgtagataaa gtttgtgcta gtacagtgtc tgttggttca
    14641 ggatttgtaa aatgtgaaca tggaattata cctatacctg cacctgcaac tatagaaatt
    14701 ttaaaaggtg tgccaattaa attaaataat gtaaatggag aatgtacaac tccaacagga
    14761 gctgcaatca taaaagtctt gtgtgatgag tttgtagatg agcttgaatt ttctccaaag
    14821 caaattggat atggtatagg tcataagaag tttgaagtac caaatatact cagaactgta
    14881 ttaggtgaaa aaaaaaagaa gaattaattt atgaaattag tgcaaacatt gatgatatgt
    14941 cttcagaaat ctattcttat ttgtatgaaa aaatattaga tgagaaagca ttggatattt
    15001 atacagagag tatttatatg aagaagaata gaccagcaac aaaagtatca attttatgta
    15061 atgaatcaga tttagaaaag tttattaaaa tattactttt agaaactagt acttttggag
    15121 tgagatataa tgcatataat agagaaattt tagagagaaa atttgtaaaa ttagatacaa
    15181 aatatggagt ggttacagta aaactagggt attataaagg aaaactagtc aaggttactc
    15241 cagaatataa tgaatgtaaa actatagcaa aaaatgaaga tattcctata aaaaaagtgt
    15301 ttgctgatat aaattttaga ataaaagaag aatttagtga aaacttattg acataagtaa
    15361 taaattttga taaattaata ttcagcctta ttaaataaaa gtaaacagaa aaaaatatat
    15421 aaaatataaa ctcactcgta tatgctcaga aatatggtct gagagtctct accaagatac
    15481 cgtaaatatc ttgactatga gtgaaattat tataccaaaa gacctagtta agtaaatgtg
    15541 taggctgagt tatactccct atgtgttggc tataagataa aggtattttg gaagcctgta
    15601 tagtaatttc acttttgcta gaaattatgg aagttactat acaggttttt tgtgtaaaaa
    15661 gatttaatat aatttagact gtattgtgtt aaaataaata tataaaaact atattataca
    15721 aggagagatg tgaatttatg aaacatgaat tagtacttgt aatagatttt ggaggacaat
    15781 ataatcagtt gatagctaga agagttagag aaaacaacgt atattgtgaa atacttccat
    15841 gtactgcaag catagaaaga ataaaagaaa aaaatcctaa agggataata tttacaggcg
    15901 gtccaaatag tgcgtattta gaagattctc caacaatatc aaaagaaata tttgaactag
    15961 gagtaccaat acttggaata tgctatggta ttcaaataat gtctcatgta ttgggtggag
    16021 ttgtaagaaa aggtaataaa caagaaaagg aatatggaaa aacatctata acttatggaa
    16081 aatcatcatt atttgaagga ataactacaa atagtgtatg gatgagtcat acagatttaa
    16141 tagaaaaagt tccagaagga tttactatag ttgcaaatac taatgattgt ccagtagcag
    16201 ctatggaaaa tgtagaacga aacctatatg gagttcaatt ccatccagag gtagaacact
    16261 gtttggaagg ggacaagata cttacaaact tcttatataa tatttgtaaa gtaaaaggtg
    16321 actggactac agattcattc atagaagata aaataaaaga attaaaagaa aaaataggcg
    16381 ataaaaaagc tttatgtgct ttaagtggag gagttgattc ttctgttgct gcagttctta
    16441 ttcataaagc aattggggac aacttaactt gtatatttgt tgaccatggt ttacttagaa
    16501 aaaatgaagg aaatgatgta gaaagaatat ttagagagaa atttgatata aatttaataa
    16561 gagttaatgc agaagataga ttcttatcta aattaaaagg agtttccgaa ccagaagcta
    16621 agagaaaaat aataggtgaa gaatttataa gagtatttga agaagaatca aataaattag
    16681 gaaaaatgga tttcttagta caaggaacaa tctatccaga tgttatagaa agtggacatg
    16741 gaaatgctgc tactataaag tctcatcata atgttggtgg aatcccagaa gatgtagact
    16801 tccaagaaat tgtagaacca ttaagagagt tatttaagga tgaagttaga aaaataggat
    16861 tagagcttgg tatagaagaa ggattaatat ttagacatcc tttcccagga ccaggtctag
    16921 gaataagagt tataggtgat gtaactaaag aaaaatgtga tatattaaga gaagctgatg
    16981 ctgtttatat ggacgagctt agaaaagcag gtttatacag agaaatatgg caagcttttg
    17041 caacattacc agatgttaag actgttggag tcatgggtga tgaaagaaca tatgcttatt
    17101 tagttggttt aagagcagtt acttcttctg atggaatgac ttctgactgg tataagatgc
    17161 cttatgatgt attagaaaga atttctaata gaataataaa tgaagttgat ggagtaaata
    17221 gagtagttta tgatataact tctaaacctc ctggaactat tgaatgggaa taaaatatag
    17281 aatgccagaa atccacatga atacaggatt tgaggtctct cttacttcta tattatactg
    17341 gtttgatact atcaatattt gccagtatct tatggattaa taagcaaaaa acatcctgct
    17401 gaatggtaaa ttcggcgggg tgtttttatt ttgttttgaa actcctttgc tctgaaactc
    17461 gaagtattta acactgatga tgttgttatc ttttagttcc ttttgtgtca tgctctgaaa
    17521 gacaaattga tacaataaaa aagtaatttc ttaatcattg atggagatat gtatttatgt
    17581 tactcttgat ggtaatttga gtaatattgt tcatagcttt ttatattcac ttaattagaa
    17641 aaccatttgg tataatcata aataaacttg atgatataat agaaaagatt tataattatt
    17701 agaagtatat aaaagtattt tatgtctatg aaatgtatgt ctataaaaca tatagatgac
    17761 ttttgtatat taatgtatga atattgatat tatagaagaa ttgagttgtc aatgatacta
    17821 aatcaaatat ttatagaaga tggagggatt atgagaaata aaataattat aggaattact
    17881 ttttcacttg tattactaat gtctggatgt gcttcaaaac aaacagcaga aataaacgta
    17941 ttagcaacta ctgatttaca tggagttata ccttatgagt taacttctta tgtaaaagaa
    18001 gaaagaaaaa aagataaaaa tctaacttta gtagatgcag gagatttttt tgatagtgga
    18061 gaagtgtttg gttctagcat ggatagttat tttagtgcta gatataaaga tggtgataat
    18121 aacactgaaa gttatattga gacgcctatt tcaaaagata tgaaagaggt aggttatgat
    18181 gttgcagttc ttgggaatca tgaatttata tccaacaata aattttatct agataatatg
    18241 atatctgatt ttgagaaaca aaaaataaat attttatcgg caaatacgta taaaaaaaat
    18301 ggagatagtt atacaaaacc atacataata aaaaatatta atacacctga aggaaacgta
    18361 aaactaggaa ttttagggct tactataaaa gaagttggtg agaggaaaca gtggaaagat
    18421 ggaaaacttg ttgactctaa gtctttagaa ctaaaagacc aagagggcta caatggtgaa
    18481 ttgtatatga atgatttagt agaagatgct aaaaagtggg ttggtataat ggaaaaagag
    18541 aagcctgaca ttatagttgc tgtggcacat acaggtgaga aacctaagaa acctagaaac
    18601 ccaggaaata gaatacaaga cttagctcaa caagttgatg gaattgatgc tatagttgca
    18661 ggtcataatc atgttcaaat tgagcaacat gattataaaa ataaatcagg agaaaatgtt
    18721 atagttactg agccaggaaa gcatggggaa tatatttcta agattaattt taaattagaa
    18781 aaaaacaatg ataaatggaa tatagttgac aaatctagta aattaattaa atttgaaaaa
    18841 aatcaagaaa gtgataaatt tggagaattt atttcttcaa ttaataaagt aagtaaagat
    18901 aagaaagaaa taagattatc agaagtaaag actcctgaat gggataaaat atatgttttt
    18961 aaatctggaa catctgcaga tgatatatat aaaacaatag gatataaatg gagaaatata
    19021 tcatctccaa agggtgatga catggtgcag attgttgtaa tgaataataa taagcctgtt
    19081 agctatatat atggtgaagc tgaagttatg ccaattaata taaaatgtga tgaatcagag
    19141 tataaaaata atgttgtaac tatttatcct aataaaaatg ataaatttaa aattgaaaaa
    19201 ggtaagcaag aatatagtat taatttaaaa catatacaat agaaactttt atcttattta
    19261 agtttagatt gtacttaaaa taaagtgtgt attattttaa aaagtgatga taattaaaat
    19321 cacaacatta aggaatagta cacacttttt atgctaaaaa atagaatgtt aaaaaattaa
    19381 acctttaaat taaagtacat tgcttatatt atccaatatt atttccaaag gttgtgtaat
    19441 atttttatcc ttatgtaata ttgctaacat tttattttcc atatctatct catctaattc
    19501 aatcaatcgc aattttttat gtaagagtga atctaataat aaattttctg gcaaaatggt
    19561 tatacctaac cctgcttctg cagctttaat aagtgcaaaa gaattaacac tttcccaaac
    19621 aggataagct ttttggtttg aaagagataa tacactgtcc aaagtatcgc gaatagcact
    19681 tccttgttca cgtaacaata acggaaacgt gcataactgt cgtagagata aggtttgttc
    19741 agataaagga aaagttggag cacatgcaac acataactta taggaaccta ataaaattgt
    19801 acggaatgcc ccctgtggtt ctataccttc ccaaaaacat atatcagctt ctccactttt
    19861 taaaatatcc atagctgaat tagcactttc gacacgtaca ttcactttaa gagatggaaa
    19921 tgaatttttt agtcgactta aaattggtgg aagtaaaaaa gaggcaattg taatgctaga
    19981 aacaatgttt ataggtgtac attcttctaa acgactgatt tttttgtcta aattgcgaca
    20041 agcagttaag atactacgtg cttcatctaa aagagaaatg ccacagcgag taagacatac
    20101 ccctcgtggt aatctgtcaa atagtgatgt ttcagcttgt tgttcaagtt cagctatagc
    20161 atgagataca gcagattgtg tgatatgtaa tttttttgca gcacctgtaa aagtgcctgt
    20221 ttgttcaatt gcttctaaaa tctctaaata tcgtatgttt atcataataa atatcctcac
    20281 tatacatgaa taaaattaat taattccatt aaattctatc attttacaga ttgcaaagca
    20341 acaggtatac tgaataatat gaagtgaaaa gaaggaggaa tattttgaat caaagcagaa
    20401 aaatatattt ttggctttta attattaact tgtttattag cacattcact tttggcggag
    20461 ggtatgtagt tgtacctatg attagacgtt attttgtaaa tcaaaagcaa ttctttacag
    20521 aagatgattt gatgagtatg gcagcagttg cacaatcaac tcctggagct attgcaatta
    20581 atttatctgc tttggcaggt tatcgagtag ctggtacttt aggggttgtc attagttgta
    20641 cagcagctat aatacctcca ctcgttattc ttggattagt atcagcattt tacaccactt
    20701 ttatatctaa tgccattgta actgcagtat taaaaggaat gcaagctggt gttgctgcac
    20761 taattgtgga tttgattatt gacatgtgct ctatgatatt aaaacaacgt tctttgtttt
    20821 tatcaactat gatacctatt gctttctttg caaactttgt gatgagtata aatgtaattt
    20881 ttatattatc tgtttgctgt tttgtatgtg taatgcaagt tttctataag aagagggaaa
    20941 ataaatgaat acaatttgga acttattttt tatattttta aaaataggct tgtttagtat
    21001 tggtggtggt tatgcaatca tacctttaat ccaagagaaa gtagtaaatc aaactggatg
    21061 gataagtgag aaaatgttta cagatattat aactatttca caaatgacac cagggccact
    21121 ggctgttaat acttctactt ttgttggact tcaaattgga gggttttggg gagcagttgc
    21181 agccacttta ggttgtgtat tatgtggtgt agtaatatct ctatttttat ataatttttt
    21241 tcaaaagcat cagaactcta cttatatatt tgaaatatta aatggtctaa aatcagcttc
    21301 attaggttta attatctcag ctgcggctac aattataatc cttgcattat ttgggacgaa
    21361 taaagtgtac ttcaattcta tatttgagat gctaaactgg actgcactaa taatcttttt
    21421 tgttatacta ctgatagtac gtaagtggaa aattaatcct attgtgatta taataataag
    21481 tggtatacta ggaatatttt tttatttata agtttttggt aagtaaagga tatctatttt
    21541 tttatatctt aaatccaaat agccaataaa gtaaggctat ttggattttt aataaattct
    21601 gaaaagataa tcagaaaccc atataacatt taaattatat cactggtttt ctacaaacca
    21661 atcataagct tcttggcaaa atttctcact atctatatag taactattat gaacattatt
    21721 aatttcaaca agtttctttt tacattttag tgaattataa tagctatcaa aatattgggt
    21781 tttaaaatat ctatcatgaa gtgttttaaa gacaagagta ggtatgttga ctccatcttt
    21841 tatcttacta tcttctgcaa taaagcattc taacattgta ttaattatct ttgtactatt
    21901 tttccaaata tgaggatttt tcttttcaaa tattttataa aattcattat catagacttc
    21961 attataacct ttatatttag gtggttttac tgttatgtga aaattaggaa gaaacttaaa
    22021 gaaaaaatat aataattttt atgttctgtt ttatatatta ttcatatttt atcacagata
    22081 atatactaaa gttaattttt ttattgtaca actattataa taattcttct ataatataag
    22141 acattctata attttatatt taagttcatg tgtatttaat tgtttttata taaaatgtcc
    22201 agtaatacta tttggattta tttttatttc atcaggagtt ccagaagcaa caatttcccc
    22261 tccatttatt ccactgtctg gacctatatc gataatccaa tcagaagcac gtataacctc
    22321 tgtattgtgt tcaataatta aaagtgaatg ctgttcattt acaagttttc taaaagtaat
    22381 caacaaattt tctatatctt taaaatgaag tccagtagta ggctcatcca atatgtacag
    22441 cattcgtttt ttagtttttg tccctaattc ttttgctaat tttatccttt gagactctcc
    22501 tccgcttaat cctaatgtat tttgtccaag ctttagatat ccaagtccca cttcatctaa
    22561 taaggagagt tttctatata tatcagagca atcactaaaa aatgtttttg cttctgctac
    22621 ttccatgtca agtatatctg caattgtttt tcccttgtat tgtatagaca gtatttcttt
    22681 ttgatatctc ttaccttgac attcattaca agttacccat tggtctgcca tatattgtag
    22741 aataattttt gttttaccta atccttgaca gtttgggcat tgcccctttt tgctattaaa
    22801 gctaaaatag ctttcatcca ataatttttg ttttgcttgt ggagtttcag taaatagaga
    22861 acgaatttca tcaaaaatat tgatatatgt agcaggtgta gaacggctag attttccaat
    22921 aggtgtttgg tccattaaga taaaatcgtc aatagcatca aatccagtaa gtgcagtata
    22981 atttttattt gggatagatt tttgtttcaa tttttcttct agagcaggaa gtaaactgtg
    23041 aaatacaaga gaactcttac cagaaccact aacacctgta atagagcaca tacagtttaa
    23101 gggaatttct acatcaatat tttttaagtt atttgcatgg catcccttta agcttaacca
    23161 tttacttggt ataattttat tttgtgaact tatcttattg tatgtactca aatatttgcc
    23221 tgttatagaa tttggatttt ttataatttc ttgggttgta ccttctgcaa tgatgaagcc
    23281 cccttttgta ccagcatttg gtcctacatc aataatataa tcagcagaca gtattgtatc
    23341 cttatcatgt tccactacta taacagtatt acccttgtct cttaattctt ctatcatttt
    23401 gatgattaga ttatggtctc ttggatgtaa accaatagat ggttcgtcta aaatataggt
    23461 taatcctact aattcacttc ctagctgact tgataatcgt actctctgca gttctcctcc
    23521 agataaagtt ggagctgttc tatctgttga tatatagcta agacctacct ttaatagata
    23581 actaactcga atttttaatt ctgttaagat atcatttacc aaagatagct catttttttg
    23641 aagttgattt ggtaattcac acaaccaatt ccataactgt tctatcgtca tttttgttag
    23701 ttcagggaag cgataacctt tgattgtagt gaatctagcc tcaatgcaca gcaattcgcc
    23761 accacatgta ttgcaaggaa ttttgttcat atattgatgc aaggtattat tttcagaact
    23821 agattctcta aaaagtcttt gaatgtggtt gattgctcct gaagcaggcc ttctaatctt
    23881 tgattctcgt ccctttgatt caaagctaaa ttcatatatc ttatcgtctg taccatataa
    23941 gattgcatct ataaaatctc ttggcaattc attccaaggt atatctaggt ctatattttt
    24001 atcttttgct attgcatata attcacctac catccaatta ccatttggct ttttccccct
    24061 caattttcca aaatagtaag tagctccatc taatattgaa atagttgggt cttttacaat
    24121 taattgagga ttaacttgat aggtaaaacc caatccatta caatcgtgac aagctccagt
    24181 atgggagtta gcattgaagg ttgaagtatt tatcttagaa aaaatagttt ggcaatgtgg
    24241 acaataattt tttgtattat tctttttttc taatggagtt ttgcaatatg gacagtaact
    24301 ttttccgatt gatacaaata acattcttaa gtaatcccaa atgcttgtaa gagtaccaac
    24361 agtagaacga ggattttggc ttcctttttt ctgttcgata gctatagtag gggttaatcc
    24421 ctgaagtaaa tcaaattccg gtttttgaag ctgttctgaa agttttgctc cattacctag
    24481 attttcaaaa taacgtcttt ttccttcttc ataaatcgta tcgaaagcta aagaagattt
    24541 tccactacca cttacacctg taataacagt tatttgattt tttggaattg taacctcaac
    24601 atcctttaaa ttgttttgcc ttcctccttt tagatagata gagcttttat tatgacaaag
    24661 tgaattttta attgatttat ttattgcttt tgcaaatttg aatttctttt ctagttcaag
    24721 aggattgcct aattctttta tttccgaaat acctaatatt tttaactgag aaaagattga
    24781 tttcaaataa tcttcaggca atttttctat taacaaatta aggtcttcta tttttatatt
    24841 tccgcttgat aatatctctg ccatttcatc taataaattt ttttctgctc ttgttaattg
    24901 attgtatttt ttggaactta attctgtcca taaatcccat ggaagcaatt ctaaaccaat
    24961 catattatta aagtcatgta gaagattacc ctttacagaa tgccaacctt tccaattttt
    25021 attatatcca aagtcatctg gatgagcatg tccttgtcga catcttatcc aggcttctga
    25081 accagttaaa aaaccatctg tttttccgac atcatgagtg tcaaaattaa tttgaaaata
    25141 atttttttgt atgtcatcta tttgtgcatc tattcttatc catctctttg ttagagtatc
    25201 atagtattct acaagccagt ggtcttcata agtaagctca ttttcaaaat aattagcaaa
    25261 acctgctctc attctagcct caaaacctcg atatcttagg agcgatacaa gaagtaaaga
    25321 ataatctctg cacattccaa caagcctttg ttttgggttt cttggaatag ttagtgggtt
    25381 agaatttatt tcaaatattc tttttaacat ttgaggaatt gtacgaagca attcctcatc
    25441 agattgttgc ttattaaagg atataccata taattttcct tggtctccat gaattactag
    25501 gctttgaacc acttttaata actcaattgg agtctttggt aaatcttgat atttattttc
    25561 aaatttttta ggatttgtga tgatgctttg atttttatag ttatttattt tcatttatat
    25621 ttcacctctc taaatggatg atataccctg acatggtgtc atatgcaagg acaatttaga
    25681 ttttttggcc aaaatttaaa aataaataaa aaatgataca gtgataaatt gtaatacaaa
    25741 tatagtttca aattgcatat aatataatta tttaataaaa ccattatcta tggaatgtta
    25801 atgaaatgtt aagatggcat tcttgaatat taagaaatag aatactataa tatagtgtga
    25861 aggattctta gtaaaaaaat ttgtaagtgt taatatacat taaaaaatag gtttataatt
    25921 tggaggaaaa aaatgaaatt atcaaggtat aagaaaaata gtttagttct gtctataatt
    25981 ttatgttcac ttattatttc aatccttgta attactgttt ttaataccgt aaaactacac
    26041 aatatatcta ataaacaatc taaaaactat ttaaacgatg tatctacaca gattgtaatg
    26101 aatgttgatt ctaaaatcaa atttatttta tccgacttaa gaataatggc agattttatt
    26161 aagcaatatg aaggtgatag tcgatatgag tatttatcta aaagaaagac tagttataag
    26221 tattatgatg ttggaataat agatttagaa ggtaaagctg agtttttaag tggaaaaaag
    26281 tttgacttaa agaatactgc ttcctataaa aaaggaattg aaggtaaaga atactcagaa
    26341 gtaatagagt caatgggttt tgttctttat agtgtaccta tatttaacga taatcatgaa
    26401 gtgtctagta ttttaatagg tgtatccaat aaagaaagca tggatgagat tcttaatata
    26461 gatagcttta atggagaagg aactattgaa attattaata gtcagggaaa aactcttttc
    26521 ataggaaaaa ataatgagtt aataaaagac cttagctata agtataagga ttctgcagat
    26581 gaaccttggg cacaaaagat gcttaatgat tttaaaaatg ataaatcagg aaatattaca
    26641 ataacctcat caaaaggtgt acaatgctta ttaacttatc atccaattac aagaggttta
    26701 aatgattggc attttcttct catagtacct gaagatgttg tacttggtga acttaataag
    26761 ttaaattctt ttacaataat aatgacattt ataactacat gtattattgg aattataaca
    26821 ttaattttat atattataag aagaaaatat gtaaatcaaa tagaaaatat tgcatattta
    26881 gatagtataa caaatggtat aaattcaact aagtttagta tgctagtcaa acctttaatt
    26941 tcagaatcac ctgatagtac ttatatgatg atagcaatga atataaaaga ttttaagcta
    27001 ataaatgatt gtttcggtag tgaaaaagga aatatgacac taaaacatct ttacaatatt
    27061 ttgtataaaa acattgatga aaatgaagaa tttgtgtgcc gtcatgatgc agaccttttc
    27121 tatttgctta ttaagaatag accagtgtta gaagccctgg agttattata taaaatagaa
    27181 gctgacacaa attattttaa taaaaatagg gaaaatccat actttttaag actatcagta
    27241 ggtatttaca caattgaaaa tcataatgaa gatttaatta caatacaaga ccatgcaaat
    27301 acagcaagaa aaagctttaa taaaactcat caaagtgatt tttcttttta tagtgatata
    27361 gaaagaaaac gattaatatc tgaaaaagaa atttcaaatt taatggaaaa agcccttgaa
    27421 aataaagaat tttttatgtg cttacaacct aaaattgata taaaaagtgg aaaaattagt
    27481 ggagcagagt ctcttgtgcg ttggaaaaat tcagaaaagg gcataattta tccaagtaat
    27541 tttattcctc ttttcgaaaa aagtggattt atatgtaagt tagatttata tatactagaa
    27601 gaaacatgca aattgatttc taagtggata aaagaaggta aagaagtgtt gagaatttct
    27661 gttaatgtat cacgtcaaca tttgaatgat aaattgtttt tagaaaaata taagcatata
    27721 tgcaataaat acaatgtacc aacatgtctg atagatttag aacttaccga atctattttc
    27781 ttagaaaatc cagaagctat agatataata gaggatattc attctaatgg atttaaatgt
    27841 tctatagacg attttggatt tgggtactca tcattaggga tattaaagga ttttaaagtt
    27901 gatataatta agttagaccg ttcattcttt gttagcaaaa ataatataga ccgtggtaag
    27961 gtagttatta agtctattat tgaacttagc aaaaggctag gtatgaaagt aactgctgaa
    28021 ggaattgaag aattagaaca agttgagttc ctaaaaaatg taggatgtga ttatattcaa
    28081 ggttatgttt tttctaaacc attattaatt tctgattttg aaaattttgc atatgaaaat
    28141 aataaaatta aaaatatcca ttattaaatg tacaagataa aaaatttagg caagttatat
    28201 aattaaaaat ataacttgtc ttttttattt atatatttaa taaaaatata tttgcagatt
    28261 ttgtaaaaat aattttcaaa tgttgtaagt tgtggctaaa gctttattgt attattctga
    28321 atatagaaag atagttactg tcttagataa aaactgaaac atacataata atataaaatt
    28381 tgctatatta ataactctaa ttattttgga aggagggaaa atatgataag tgggagaatg
    28441 ttactcatca tgaattttct aaaaggaaaa aataagacaa gttacaagga tattagcaaa
    28501 caacttagta tagaagaaag aaaagttaga tatgatataa ataatttaaa tattgttttg
    28561 cgttcattaa acaagtcagt aatacaaaag atgaacaaag gtgtgttaat tattcctgat
    28621 gacttccaag tagtatgtat aaataataac gaatatgtat tttccttaac tgagcgagta
    28681 tctattatga aaattatatc aatgtttaaa atagataatc taaacttaga aaaattgagt
    28741 aaagaatttc aggtttctag aagttcaatt aaaaatgacc ttaatgtttt gtcaagtgaa
    28801 ttagaaagaa accaaataac tattacttat gataaatctt ttaaaataag tggagaagaa
    28861 gaccttatat ataagcaaag attgaacata ttaaaatcgt attcttatct ttttggtaag
    28921 gaaaaatctg atttaagtgt atttgagaag tatttagtga atgtaattga aaaaatattc
    28981 aaagggattt atttgattga tatatataat tggtctatga atttattatt gcaaatggga
    29041 tggactttaa atgatgaatc ttttacatgg tatgtgtcaa acattttcct ttttacatgg
    29101 tatatacatt cagatgaaaa acatccttta gaatctgctt cattaagtga gccatgtttt
    29161 gaaaataagt ttgaccaaga atttgaaaca attattcaaa aaaagttgac taaggaacaa
    29221 atttttctgt taaagagttt tgtatttttt acaaataaat atgcaagttt aaacgaggat
    29281 atggatttga ttagtacaga aattatagtt caagacctaa tttacaatat gtcagaaata
    29341 ttagagatta gctttaaaga agatatgatt ctttataaag gattactcaa tcatattgcc
    29401 ccattgatag agagaatcaa gcagaatata cagatttatg aggaattact tcatgtaata
    29461 ccagataaat atcaatatgt acttgaagct actaaggttt ctattcaaaa aaacagagtt
    29521 ttaagtcaaa taaagaatga aaatgaaata atacttttgg caatacattt cttaggaagt
    29581 atacatagaa atgagaggaa taactacaaa aatatattgt tagtttgtgg atttggatat
    29641 ggtgtaattg ctatgttaaa ggatagttta gtaaatgatt accagataaa tatagtagag
    29701 agtattccat catataagtt acaagattat aaagaatggg aaagtattga cttcattata
    29761 acaacatcaa agatagtaat agacttgcct aaaacaatta ttgagataaa tccattttta
    29821 caagaagagg attatttgaa gtttgaagaa aaagggataa aaagaaagaa cgtcttaact
    29881 aattatatgt caataaaaaa aaggttggac tttttagaag aagatgctca aaaaagagta
    29941 ttaagtatta tatgtcaaga acttggatac agtgatgaga gaatattatt tagacaactt
    30001 aatttaagtg atttaatagg gattgatacc ataaggataa ttgatgaaga tatgaaatgg
    30061 gaagatgctg taaaaattag ttcaaacata cttgctaatt gtgattttgt atcagcacaa
    30121 tatgctgaga atattataga tattttaaaa aatgtgggtt tttatgctgt aaaagataac
    30181 gaatttgctt tactacatgg caatgatagt tcattagtca aagtaagttc gataagttta
    30241 ttaatatgta aagaagcagt tagttttgga gataaaaagg ttaaaatcat attttgtcta
    30301 gcaagcagag ataaaaaaga gcaaattcct gcaataatta atctaactaa aatggtttat
    30361 aagactaatt ttattgctat gttagaaagt gcaaagacaa aagaagaagc agaagtttta
    30421 attcataaat atgagaagga ggtcactgta tgaataaatt agttaatgta aaatgttcat
    30481 ttaaaataca tgctaattca aaagaagaag ctattgtatc gttagttgat gtagtatcaa
    30541 aagaaggata tctaatagat aaaaatcaat ttttggaaga tgttttaaag agagaagaaa
    30601 cattgtcaac ttatattgga catggtatag gtcttcctca tagtcaaagt gtaggtgtta
    30661 aaaactcttg tattacaata ggaaagcttg atattcctat tgaatggaca gaagaaggag
    30721 aaaaagtgga tttaatattt ttaatatcag taacaaaaga caatgaaaat aatcttcatt
    30781 taaagatttt atcaaaatta gcacgtttat tgatgcatga aagttttaga aatcaaatta
    30841 gatgttcaga tgaacaaact gtttataatc ttataaaaga aaaaatagag gaggaagatt
    30901 aaaatgagaa ttttaaagtt tttgcaaaaa catcttatga cagcaacatc ttatatgatt
    30961 ccatttgtgg ttgcaggagg aatattattt gctttatcag taactctaag tggacaagct
    31021 gctgttcctg aaacaggctg gttggctaaa ctgaatcaaa ttggagctgc tggcttggct
    31081 ttattcattc ctatattagg tggatatata gcatttagta tggcagataa accaggatta
    31141 gctccaggta tgataggtgc atatttagca aaagaagtaa atgcaggttt tataggtggt
    31201 atcatagcag gatttatagc aggatttgtt gttttacaat taaagaaaat taaattagct
    31261 cctacaatga gaactttagg ttctatattt atttatcctt tgattggtac attgattaca
    31321 ggtggtataa ttgtattttt aattggagaa cctatagctt cttttatgac atggatgaca
    31381 aactggttaa atggaatgtc tggtgtttct aaaattcctt taggtggaat cttaggagga
    31441 atgatagcct cagatatggg tggtcctata aataaggttg ctgctacttt tgcccaaact
    31501 caagttgaca cacttccata tttaatgggt ggagttggcg ttgcaatttg tatacctcca
    31561 attggtttag gattagctac attattgttt ccaaaaaaat ttagcaagga agaaagagat
    31621 tcaggtaaag cttcgctatt aatgggatgt gttggaatta cagaaggagc tattccattt
    31681 gcaacatcag atccagttag agtaatacct tgtattatgg ctgggtcgat tataggaaat
    31741 attatggctt ttttattagg atgtttaaat catgcaccct ggggaggtct gatagtatta
    31801 cctgtagttg acaataggtt ggggtatatt gcatcagtat taactggagc agtagttgtt
    31861 gcagtattaa tgaaattagt aaaaaaagac gtgaaagaag atgaagaaat tgaagaggat
    31921 ttagatgaat caattgaatt aatatttgaa gaattataaa tataaatgga aggaatttgt
    31981 ataaaaaagt aaatataaaa ctataaactt aaaggagata agaatatgaa aattgtagca
    32041 gtaacatctt gtcctagtgg agtagcacat acttacatgt cggctgaatc acttgaatta
    32101 tcggccaaaa aatttggtat agaaataaag gttgaaactc aaggaagctc tggaatagat
    32161 aatgaattgt cacttaaaga tattgatgaa gcaacttgtg taatattgac taatgatgtt
    32221 gagattagaa atatggaacg ttttaaggga aagaaggtag taagaatgag tgtatctgag
    32281 ataattaaga aatctgatgc attaattaag aaaataaaag atacttttca ataaaaattg
    32341 atttatttta catataactc aaagatgttt ttaaagaaat ttttattaaa agcatctttg
    32401 aaaatatctt aaaaatagaa taaaaggaga tatagtcatg aaaaaattac caataaagac
    32461 tgtcgtacaa agattactag acttacaaga agaggggaaa agtgcaactc ttttaggaat
    32521 tggtcctatg tcaccaaact tgttacaagc aagttttgaa ctagcaaaag atgatgattt
    32581 tcctttaatg tttatcgcaa gtagaaatca agtagatgca gatgaactag gtggcggtta
    32641 tgtaaatggt tggaatcagg aaacttttac aaaagatatt aagaagattg ccgacaaagt
    32701 tggatttgat ggtttatatt atttatgtag agaccatggt gggccatggc aaagagataa
    32761 agagagaaat gaccatttgt ctgttgatga agcaatggag ttaggaaaaa aatcttattt
    32821 agcggacatt gaagcaggtt ttgacctatt gatgattgac cctacaaaag acccatttga
    32881 aattggaaaa gtaattccac tagatgtagt actagaaaga actgtagaat taattgagta
    32941 ttgtgaaaac gagagaaaaa gacttaatct accagacata ggttatgagg ttggtacaga
    33001 ggaaaccaat ggaggtctta cttcaactga gacgtatgaa acatttataa ctagactaaa
    33061 ggttgaactt gataataggg gtttgccaat gccaacattt atagttggtc aaactggtac
    33121 tttaactaga aagacagaac aagtaggaac ttttaatttt agaaatgctt atgatttagc
    33181 tcaaatggct aagaaatatg gagttggttt aaaagagcat aatggcgatt acttagatga
    33241 tgtaacttta ttagaacata taccatctca aattattgct acaaatgtag ctcctcaata
    33301 tggtactgaa gaaactaggg catatttaaa attagcagaa gttgagtgta aattagaaaa
    33361 agagggccta gttgataaaa cttctaatat cagacatgtt cttcttgtta atgcaattga
    33421 atgtggacgt tggagaaaat gggtagtagg agaacaaaaa aatctaacta ctgaagagat
    33481 atttaagaat gaagttttat ctaatgaaat actagatatt gcaggacatt atacatttaa
    33541 taatgatgat gttaaaaaag aaatagaagt gctttatgat aatttaagta agaataatat
    33601 tgatggtcaa agatttgttg tagaccatat taaacgtcct ttgcgtgatt atgcagaatg
    33661 ctataactta aaaggtgtta ctacgaggat tttaaataaa taataggact tatagtggtg
    33721 agtattgtta tactaggtat ttatagttat aattatactt accactatta aatttaggag
    33781 gactaatatt ttgattaatt taatttttga tgtagatgat actttatata accaattaac
    33841 gcctttttac actgcatata ataaagtgtt ttcttcaata aaggatattt ctattgaaga
    33901 tttatatatg agtagtagaa agtatagtga tgaggtattt catatgactg aaaatggaga
    33961 aatgccaatc aaagaaatgc atatttatag gataatgaaa gcttttgagg agttgggaaa
    34021 ttctattaca gaaaaagatg cacaaagttt tcaagatgag tatatatatc agcaatcaca
    34081 aattacacta ataccagaag ttgagaggat tttaaatttt tctaaagaaa gaaatattaa
    34141 tctaggaatt ataactaatg gcccatcaaa ccatcaaaga atgaagttaa agcagttaaa
    34201 tattgaaaat tgggttgata aaagtaatat ttttatttct agtgaggttg gattttctaa
    34261 gccagatact aatattttta gagtagctga gaatgttatg aatttagata gagaaaatac
    34321 atattatgtg ggtgattctt atagaaatga tgtattaggt gctaaaaaag ctggttggaa
    34381 gagtatatgg ttgaaccata gaggacatga ggtagaagag ttgttttata aacctgattt
    34441 tgtaattttg gaacataagg atttgatatc tttatttata aaaatatgta gttataaaaa
    34501 tatgtagtaa aaaaatgtta ataatagaat tgtataaaat aattaataat aactatataa
    34561 aattatatgt ttagattgtg agcaatctat tttatgttaa aaatagatta tttagaagaa
    34621 ggggaaatct taaaaaaatt tgaatatccc ctttttatta tttaattaaa atgaaatagt
    34681 tagaaatata ttttatttag tttagtcaat aacattttgg gattctttat aactacatgt
    34741 taactgttga ataggcttat catatggttt aacccatcct ttattaatac ttaattctgt
    34801 aagtgctgtg tgaccttcta ccatttgagt tagtgaagct gaatatatag ctcttaattc
    34861 aggtgtacta cttgttaatg cagaattaag atacatattg gcagatgctt tagcagatga
    34921 aagcatactt aacattatta ctttatcgtc tatataagta ttgttcttaa ttagattttc
    34981 tatcatattt ctcatgttat ctatacctcc tcagaaatta gtatttcatt ttcaactata
    35041 aattgttgta tagctttaat tcttccttct gttgctaata tactagattc tgattgtctt
    35101 tttaggtctt catctgatat taacatattt attgctcttt gcataagtaa tccgtcagac
    35161 tccattttta taactgctgc taaagatatt ttttcagttt ctgaaagtgt tctcatttaa
    35221 tcatctccta tattgtaatt tatgctttaa tagtatgtct ttcttttcaa ggaaataacc
    35281 attacacttt tttacagtat tttatattct ctttatatca aagtttagtc aacttaatat
    35341 attaatataa ttttgtagga tatttatcat taaatatcta taaaataaat tatttgaata
    35401 ttcataaatc aaaatttatt tgtatgtact tcttcaagag tcaatatgaa ttttttcaaa
    35461 aaattaattt tgatgataat cattaagggc tatatttttc ctaaagttgt gattatcatt
    35521 attaatttac taaaatactt taatgaaaaa tagctaactt taattgtagg atattcttaa
    35581 tatatttgaa ataaataaaa atattgaagg atataatatt caatt
//
LOCUS       DAEPLF010000020        34285 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEPLF010000020 DAEPLF010000000
VERSION     DAEPLF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.020"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            165..794
                     /locus_tag="KR314_000860"
     CDS             165..794
                     /locus_tag="KR314_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0327259.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(908..2422)
                     /gene="glpK"
                     /locus_tag="KR314_000861"
     CDS             complement(908..2422)
                     /gene="glpK"
                     /locus_tag="KR314_000861"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBH0327260.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            2665..3207
                     /locus_tag="KR314_000862"
     CDS             2665..3207
                     /locus_tag="KR314_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0327261.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3221..5713
                     /locus_tag="KR314_000863"
     CDS             3221..5713
                     /locus_tag="KR314_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0327262.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSINQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            6134..6532
                     /locus_tag="KR314_000864"
     CDS             6134..6532
                     /locus_tag="KR314_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0327263.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMMILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(6749..6958)
                     /locus_tag="KR314_000865"
     CDS             complement(6749..6958)
                     /locus_tag="KR314_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327264.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(6990..7199)
                     /locus_tag="KR314_000866"
     CDS             complement(6990..7199)
                     /locus_tag="KR314_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0327265.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            7498..7917
                     /locus_tag="KR314_000867"
     CDS             7498..7917
                     /locus_tag="KR314_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0327266.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            8145..8600
                     /locus_tag="KR314_000868"
     CDS             8145..8600
                     /locus_tag="KR314_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327267.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(8825..9085)
                     /locus_tag="KR314_000869"
     CDS             complement(8825..9085)
                     /locus_tag="KR314_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327268.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(9348..9701)
                     /locus_tag="KR314_000870"
     CDS             complement(9348..9701)
                     /locus_tag="KR314_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327269.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQAKQDDDITNNFI
                     ANAEIYEMLLSQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGRKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(9718..14895)
                     /locus_tag="KR314_000871"
     CDS             complement(9718..14895)
                     /locus_tag="KR314_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HBH0327270.1"
                     /translation="MKQNKLLQRGAYFNDKNILIDDFDKRYNDYDFVEFFTGISNSTF
                     GLKSDGNLYACGDNTGFQLGLGKDSSERRMFSKVKIDNVKYVSCGSKHSVAVTKDGFA
                     YGAGTSNVGQLGVIESTVYYEFTKLPIDDVKTVACGYDFTFVLKNDGTLYSAGLNSSG
                     QLGLGDTNNRATFTKVNIDSVKDVVTYNQSVFIIKMDGTAHACGLNSNGQLGINSTLN
                     KSVFNKIEGMDNVKQIACGSSHTILIKNDGTMYTTGYNGVGQLGTGNNNNSIVFTLSS
                     INNVKYASCGNNHTMILKYDNTLFSTGQNNYGQLANANKDVASRNTFAKVNVENIKDI
                     KCGSQFNFLINGSKEIFVSGCNLAGQLGSFFHTTFLYEFSNVQSSNLDNYSGLLVNDD
                     YLYVTKDNSEFLNVKLSDNFQDYKKIELTDSNMFIVMNDGTLYACGLNNYGQLGLGDT
                     VNRSVMTKVDIDNVLDIKGNGNSTFVLKNNGTLYSCGLNSNGQLGLRDEVNRNIFTKI
                     EIENVKDFCVGSNYVIALNHSKEVYGWGNNPYNNIEKTSNYPYKQGISNIEKIAAYDY
                     SVYMINSEGKLYVSGYNYNYQLGKGNNSNQSKALVSQCRTNSTSSTSNGLRTLPKITN
                     VFPFYDGCAIIDEGGYVYLTGYHGYLRTLNSSPSISDYSRYGTFIEATNSNHNTYFIQ
                     ETDFSGIEKVIGMSNNILFFKKGSSYITGYPKTFGSTITGHRSYTSINSESSNLGSNF
                     IIYHSNSKLYGKGIANSGQFGNSTNIDGTSNYDTGLKDIKDIIVKGNTVVVVDKNNNI
                     YVTGMNQNNKLGIGEYNNEPVKKFTNITEQSNSFIFMDDIKEITTSRNTMFIVKNDGT
                     AYATGNNSSGQLGLGDTINRNKFTQINLDNIKKISTSIDGNTTFAIRNDGTLYSTGLN
                     TKGQLGLGDIVNRNTFTKVNIQNVRDVVLGTTHSHAIKDDNTLYSCGENTHGQLGLGS
                     ESNHPDVLTFTVNNITNVRDVYCSDTTTFIVKDTNIAYCCGYNNNSQLGMGNTTDQYS
                     FIKCMENVKEVIPNEINTYIITIYNTAYSTGLNTDYCLGLNSNSNQSSFSEIPISNVV
                     KVAPNRNNAVLLLTSEGDVYTAGKCSNGSGTGSETPEKIKKIASKAKDIGMNYRCGHY
                     VSDNGDLYGTGFNNNGQLGVGDVTKRDTFIKTNTRVKKILPLEYANIAIKDTNDIYIC
                     GLNNYGQLGVGNRYDSRNNDNRIFNYKHMNFVMGDLTSIKNRHNFILLNNKIVIPTTK
                     DIDYGLVLGNLYKGDLYTELPYEDIKEVSISKTHIIILLNDGTMYGCGTNYHGELLQD
                     LSINQVDEFVQINVSDVKHVSCGDNFTYFIKSDDSLWSIGKNSEYQLGIGHNNPVTEL
                     QRITTISSCKEVHCGKNYTLVVTTGNELFVQGYNDKGALGLGSDSENTIIKFFTKALT
                     DIREIKSYGSDHILVLKNDNSVWVTGKNRDVYKIEQPVEFLKEFTIVPISEDVNTVKD
                     VLATDNTLYIISEVGTTNAAIEITEKSISSIKIKIQDPNKDISRIEMLINGESVKSVS
                     DLTTEKISFEVPPDKIKIGENKILFRAYCKGDDLYASLFIFKESTGNSIIKDSYVMIG
                     NRMYKVVNTTSNEQDITITLDRGLEEDLNLGDPIYQLINKTKVQVKINKSDLFKDMKL
                     VEIKKSDSSYQEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(14917..15903)
                     /locus_tag="KR314_000872"
     CDS             complement(14917..15903)
                     /locus_tag="KR314_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH0327271.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGDTDILTTDSKDLSGAINEVVKKIENISFDDVISGQIQTDISVLKNSY
                     NKLSEKVLDILIYLELESEVTVDEAGYWYDTLANGNNIVAIEGLKLDLNRKCITGEIG
                     NVIFRDVVLPFSANRVRYIHDMDNNFVETKSSNTYLKEQKDITLSKYSYEIR"
     gene            complement(15904..16602)
                     /locus_tag="KR314_000873"
     CDS             complement(15904..16602)
                     /locus_tag="KR314_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBH0327272.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSEMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(16599..17651)
                     /locus_tag="KR314_000874"
     CDS             complement(16599..17651)
                     /locus_tag="KR314_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBH0327273.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGENNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(17644..18072)
                     /locus_tag="KR314_000875"
     CDS             complement(17644..18072)
                     /locus_tag="KR314_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBH0327274.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(18072..18398)
                     /locus_tag="KR314_000876"
     CDS             complement(18072..18398)
                     /locus_tag="KR314_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBH0327275.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(18414..19943)
                     /locus_tag="KR314_000877"
     CDS             complement(18414..19943)
                     /locus_tag="KR314_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH0327276.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(20225..20647)
                     /locus_tag="KR314_000878"
     CDS             complement(20225..20647)
                     /locus_tag="KR314_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327277.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEVATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(20647..23100)
                     /locus_tag="KR314_000879"
     CDS             complement(20647..23100)
                     /locus_tag="KR314_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327278.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASSRVNSLNSSLRKVQTTASSVSSTMAKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKRVEKRPYQGSINLKDMVSSAMARILPKLMLFKNT
                     FWSGVIAIKDMASSIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKTSQKDVDKYYDSLVNMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSSKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(23100..23267)
                     /locus_tag="KR314_000880"
     CDS             complement(23100..23267)
                     /locus_tag="KR314_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327279.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(23294..23740)
                     /locus_tag="KR314_000881"
     CDS             complement(23294..23740)
                     /locus_tag="KR314_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH0327280.1"
                     /translation="MNENGLSKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(23854..24282)
                     /locus_tag="KR314_000882"
     CDS             complement(23854..24282)
                     /locus_tag="KR314_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBH0327281.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(24296..25360)
                     /locus_tag="KR314_000883"
     CDS             complement(24296..25360)
                     /locus_tag="KR314_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBH0327282.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTEEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(25365..25808)
                     /locus_tag="KR314_000884"
     CDS             complement(25365..25808)
                     /locus_tag="KR314_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327283.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTITLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(26057..26497)
                     /locus_tag="KR314_000885"
     CDS             complement(26057..26497)
                     /locus_tag="KR314_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327284.1"
                     /translation="MENKKDILFKETDERLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(26602..26799)
                     /locus_tag="KR314_000886"
     CDS             complement(26602..26799)
                     /locus_tag="KR314_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0327285.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            26968..27288
                     /locus_tag="KR314_000887"
     CDS             26968..27288
                     /locus_tag="KR314_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBH0327286.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            27370..27876
                     /locus_tag="KR314_000888"
     CDS             27370..27876
                     /locus_tag="KR314_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327287.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            28577..29332
                     /locus_tag="KR314_000889"
     CDS             28577..29332
                     /locus_tag="KR314_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0327288.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(29475..29903)
                     /locus_tag="KR314_000890"
     CDS             complement(29475..29903)
                     /locus_tag="KR314_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBH0327289.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(30324..30524)
                     /locus_tag="KR314_000891"
     CDS             complement(30324..30524)
                     /locus_tag="KR314_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH0327290.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(30707..31876)
                     /locus_tag="KR314_000892"
     CDS             complement(30707..31876)
                     /locus_tag="KR314_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBH0327291.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSEDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            32112..32933
                     /locus_tag="KR314_000893"
     CDS             32112..32933
                     /locus_tag="KR314_000893"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBH0327292.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(33009..>34285)
                     /locus_tag="KR314_000894"
     CDS             complement(33009..>34285)
                     /locus_tag="KR314_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBH0327293.1"
                     /translation="LSFSVLLSSGVILPANYSEKQFDKYKEKIISSEKAIEDIIPNLY
                     EYGVYTLDGNLISGTFNKKESKQVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKLV
                     SEYSSPVLRAYLPKPEMLGMIIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQ
                     QDLDFVVESSKIYEINNILFSMDKMKLALKNSLEKQWMLEENRKEQISALAHDIKTPL
                     TIIHGNTDLLIEINENPELLEYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAIN
                     MSEFIKDVLLQIEALASTKNLDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSP
                     SQSKLYISVSVSNKYLKFVVTDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYI
                     VNNIVQKHNGILYIENSVKTGGAMVTIEIPIV"
BASE COUNT        11248 a         5710 c         3870 g        13457 t
ORIGIN      
        1 tttaagtata ataaaatcat gtatactaaa tgcatgttaa aatattattt taaaacatgc
       61 acaatatttg tacatattgt agacatataa acctataaat tatacaatag tattgttaag
      121 attatatatt tttttaacat aaattttgta ttgggggaaa aacaatggat aaaaactata
      181 cgatgccaat ataccaaaaa atagcgttgg atattgcaaa taaaatatac acgggtgaaa
      241 tacaagagga ttcagtacta tttggacgct ctgttttggc tggtaaatac aatgtatctc
      301 cagaaacgat aagaagagct gtcaaaattt tagaggacat tggcgttgtt aaaagcataa
      361 agggtaaagg tgttattgta ctatctccag acaaagcttc ttcttttata aaaaaatata
      421 gagatattac aaatatatca tcatataaat caacacttta taatttaata gatactaaat
      481 ctaatttaga aaatgaaata ttagatacta taaataaaat tttagactat tccaacagac
      541 ttgaaataat caatccttta gtgcccgttc agttcaccat aaattctaat tgcaaatata
      601 ttggtcaaac agctgcacaa actaaatttt ggcaaaatac aggtgctaca atagttgcaa
      661 ttaaaagagg tgaagaactt ataatatctc ctggaccata tattgaattt ttagaaggag
      721 acattctctt agtagttggt gaccaacaca tttacaattc tataccaatg tttttatatg
      781 aaaacgaaaa ataaattata taatttagta tgattatata aaaatgcaaa attaacaaca
      841 aaaaaagagc ttcatatttt acataaagct ctttttaaat tcaaataaat ttaactttga
      901 ttaattttta aatatccaat tcatcttctt tttcccagct taaagctctc gatacagctt
      961 ttttccatcc tttatataat ttacatctct tctcttcact catatttgga ttaaactctt
     1021 tttctactga ccactttgaa gtaatttcat ttcttccaat atagaatcca acagacaacc
     1081 ctgctaaata agcagctcct aaagctgtag tttctactat tttgggtcta tctatatcta
     1141 catttaatat atctgattga aattgcatta aaaaattatt attacttgct ccaccatcaa
     1201 cttttaatga ttttagtttt aattttgaat cgttttgcat tgcttctaaa acatcctttg
     1261 tctgataagc aattgattcc aatgtggctc ttactatatg ttcttttttt gctcctcttg
     1321 taagtcctaa aattgaaccc ctagcataca tatcccaata tggagctcca agacctgtaa
     1381 atgctggcac tacatataca ccattagtat cctctaccct atttgcatac atttcactat
     1441 cttttgcaga ttcaattatc tttaattcat ctctaagcca ttgaacagat gctcctccaa
     1501 taaatatact tccttctaat gcatatgtta cttttccatc aactccccaa gcaattgttg
     1561 taagtaaccc atgcttagat ttaactatgt tactgcctgt attcataagt aagaaacaac
     1621 ccgttccata agtatttttt gcagtacctt cttcaacaca attttgacca aataatgctg
     1681 cttgttggtc tccagcacat cctgcaatag gtattttagc tcctgataac atgcgctcat
     1741 ctgtatgacc ataaacatag ctagaaggtt ttactaatgg caacatgctt attggtatgt
     1801 ccaattctcg taatatatcc tcatcccatt ctaaatcatt tatattgtac atcattgttc
     1861 tagatgcatt tgagtaatct gttacatgta ctttcccacg agttaaattc catattaacc
     1921 atgtatctat agttccaaat aataactctc cttgttctgc tttttcccta gctccttcta
     1981 cattatctaa tatccatttt acttttgttg ctgaaaagta tgcatctatt aaaagacctg
     2041 ttttttcttt tattgattct tccaatcctt tatttttaag ttcatcacat atttctgatg
     2101 ttctcctaca ctgccatact attgcgttat aaataggttt accagtatac ctactccaaa
     2161 caaccgtagt ctctctttga ttagttatcc ctattgaagc aatttcttct ggtcttatac
     2221 ctgctgtttc tataacttct ctcattacac cactttgtga cccccatatt tccatagggt
     2281 catgctctac ccatccagct tttggataaa tttgattgaa ctctttttgt gcaattttca
     2341 ctatttcgcc ttttttatta aatagtatag ctcttgaact ggttgttcct tggtctaatg
     2401 ccattacata cttcttttcc atcaccatat tctccttatc atataatatt taatttatta
     2461 aaaagaaaaa tttaatatca catatttcaa taaataatca tacttaagta caaatttaat
     2521 ataatagata tttataaaaa gacacttatt ttctatcata tctatataat aatattatac
     2581 attatagctt acatattgat taataaattt taaaggagta taataaacag tgttcataat
     2641 aattttaata aaagaggtgg taatatgcca aagattttag aaaatgtaaa agaagatata
     2701 ttaaaagtat ctcgggatat gattttagaa gaagattact cgaatattag cataaggaaa
     2761 atagctcaaa ggtgtggtat aagtgcaggt acagtatata attactttaa ttcaaaacaa
     2821 gaaataatag aatatatcac aaaatctgaa tgggatttat tgataaggcg aattgaatac
     2881 tccaataaaa acactgaaga ttacataaaa aaactaaaca ttatttttac tgaaataaga
     2941 aattttataa acaaagttca taatatccaa tacaatgact ttttaaacac ttttgaaaca
     3001 gagagatttt ttgaaatgaa aaaacacaaa gacgactttc atgaacagct ttcaaataaa
     3061 gtatatgaag ctttagaaaa agaaagtttt tttaataatg acaaattagt atgcaatatc
     3121 ataataaaaa tgtttttttc atattcaaca tatcttcata tagaatttga agatttaaaa
     3181 ccctatattg aaaaattaat acattaaggt ggtgtttacc ttgaatcata atactagtat
     3241 taagaacaag gacagtaatc caaaaatatt tttagtactt acactttatc tattaggtat
     3301 atttatggga gctattgata ctggaatagt atcaccagca agaacgatta tacaaaatag
     3361 ccttggagta aatgaaaaaa cgggaatttg gatgattacc atatacacat taacatacgc
     3421 ttctgtaatt cctatctctg gaaaattagc agataaaata ggaagaaaat atgtgtatct
     3481 agttagtata tttctatttg gtagtggttc attaatatgt ggattatcat cactattttc
     3541 caatttctat attttattaa ttggtcgtgt tattcaagca attggtggtg gtggaatcat
     3601 gccaattgct acagcagaat ttggaactac cttcccagaa aataaaagag gcatggcttt
     3661 aggtttagtt ggggcaacat atggtattgc caatatttta ggttcaagca ttggtagcac
     3721 aattctaagt atctttggaa ctcaaaattg gaagtggcta ttttttgtaa accttccaat
     3781 ttgtttaatc atacttattg gtggagtttt ctgtattaaa aacaataagt ctgaatctaa
     3841 agaaaaaata gataagcttg gtacattgat gttgatatgt ataatcgtat cactattata
     3901 tggtcttatg aatatagact tttttaattt taaaaattct atacaagata tatcagttta
     3961 tccatactta ttgacattta ttgtacttat acctattttc atatttattg aaaatagagc
     4021 taaagaccct attttaagct ttgaatattt tttaaatccg agaatattga tagtacttat
     4081 tttatcttta attgttggta ttggaatgat gggtatggta ttcgttccac agtatgctga
     4141 aaatgcatta aagataaatg ctggttctgg tggatatttt gtagctatat tgggattatt
     4201 tgcaggtatt gctgctcctt taagcggaag acttattgac aagtttgggg caaagaaaat
     4261 actattacta ggattttctg tatctatgat aggctctctt tacctagttt taattgctct
     4321 aaaaaccaat actgtattta gtgtatgtgt aagtttaata tttatgggat taggaatggg
     4381 ctttactatg ggaactcctc taaactatat gatgttatca aatacaagat tagaagaatc
     4441 taattcagca cttgcaacat tatccttgat acgctcaatt ggtacttcaa tttctccagc
     4501 tataatgatt ggatttatag cacatgcagg attatctgtg caagataata ttatggactt
     4561 agttggaaag ccctcaactc ctaaaatagt tcaacttgaa gaacttgaaa acatgataga
     4621 tgacttaaaa tcagaccctg aaatggcaaa aaatttaaaa aatgtctcta tcccaaatat
     4681 gaatgatact tcaaatataa atatggacat gacaggtggc aaacttccaa aatccttact
     4741 taataaagtt cagtcagcag atacaaccaa cattaccaat ataacaaaag aaatatcaac
     4801 aacaatgttt gatgaaaaag ttccatctat aattactaaa atacagaaaa atgtacaaaa
     4861 aggaatagat ggaacacaaa gtggaataaa cggtattgaa gatggtgaaa ataaactaaa
     4921 tagtggtatt aaaggagttc aaactggtat tgacaatatg gtaaaagcta gagctggtat
     4981 taatcaagga atagatggtg taaaaaaagg tattgctggt gttgacaaag gtataaaagg
     5041 tatggaacaa ggtcttgaaa aacaagatga ggccatcaag gaacttacaa ctgcttataa
     5101 taaaatacct actacagaaa agactcctac tgataacgaa aataactcaa atgaacaaaa
     5161 ccagagtaat accagtaatt ctgaatcaga cgacactaag aatgattcta cagaaaataa
     5221 gaataacaat tccagcgaga ataaaccaaa tgtaaatgaa caaaaagaaa gtttaaatgc
     5281 tcaaatacag caactaaaaa agacaagaga tgacttaaat acaaagttac aaaaaaataa
     5341 aactcaaaag aacgaattaa gtaaaaaact aagatcaatg gaatctcaaa agaaagagct
     5401 acaaagcaag ttaaataatt ctataaacca aaagaaagac atggaaaaaa gccttgatac
     5461 aatgcaacag caaaaaaaat cactccaagc agttttagca aagacacaag aggtaaaaac
     5521 tgaaatacca aaggcttttg ataaatcaaa attggattat ataaattcca tagaaaataa
     5581 tagaacaaaa atagaaaata cactacaatc tactctaaac tcaggattca aacagatgta
     5641 tataacagta ttctgtgtaa atctactagc atttataata ttattgttct ataaagaaaa
     5701 taaaactaga taaaagagtg ttatagtatt atattgtaaa tttatcttat tcttaaatat
     5761 tttaccctaa agaataaatt cataaaatct ttgtaattga ttaatataaa ttacaatata
     5821 atactatttt tatcttaatc tttttctctt ttacagacaa tcaaaaagca atcttagttt
     5881 atcgaaataa atttatacaa tctattcata caccccttta catttttata acaattaaat
     5941 ctattgttat atatacaaat caaaataaac catatatttt caactataat atgttaaaat
     6001 catattatga gcatattaat ttaagcaaaa tgtcatttag actttaattt agtagtttta
     6061 tattataaat ttatatattt ttaatttcaa atgtcattta gacattactc ataaaaagaa
     6121 aggagtgtat tttgtggata ctttaggaaa aagaattgct tatttgagaa actccaaaaa
     6181 acttactcag cgcaaactta tggatatttt aaaatttgaa aacttaggca agtatgaaac
     6241 aggagataga aaacctaatt gtgatatact aatgtcaata gctgattatt ttaatgttac
     6301 gactgactgg cttctctatg gtaaagagaa ggtaaatgtc aatagtagtg taaaagaaga
     6361 taaagaagat tacttacatg ttactaatga tgaaatgatg atattaaatc tatacagaca
     6421 actaaacgaa cgtgataaaa taaaaataga aggaatactt gaacttaaga tatctgaata
     6481 taaagactta aaaaagcatt cctctaataa taatgaagat aaaatggttt aaacatacat
     6541 aatttgatac gattttacat catctattaa ccttcttttt ttgtagtata ttactaaaat
     6601 atggatattg gattatttaa aaacaagtat agtctaattt caagcacatt tataatattt
     6661 agattatctt atccatattt tagtttaagt actttgaaaa atataataca tttctatgta
     6721 taatttatat tcctaagaaa tgcattactc atgtttttaa ttcttccaac atctttctat
     6781 acacaattgt acatccttca taattcccct ctttaagaag gattgaacat gtactagaat
     6841 atttagttaa agtatttaat ctttccatat ttaagctttt tatattacta agtgctgtaa
     6901 taaaatcatc aattgctcta caacactcta taaccccatc tgaatccaat ttgcacacaa
     6961 gaatattcac ctactttata tactatttat taatcatctt ctgaatattc tatattaagt
     7021 aattgataaa ttgatgattt atatttttta cctgttcttc ttccatttaa aatagctgac
     7081 aaatagtttt cattaacacc tattttcttc gctaattccc tttgagtcat atttaattca
     7141 ataagtcttt gcttgacttg cactcccaaa aaaggtatcc atttttttcg agcatccatt
     7201 tatcaacttc ctttctaagt gatttatatt tacttttata ttactttagc ttacgcaccc
     7261 cctctattta gtatatattt ttaatatgta cacatattaa gtatataaat ttatttaata
     7321 ctaaataaga ttttttgatt actttgtgct aaaattagga tgtaaattcg tatcattaag
     7381 ttaattataa agtcattttg accttaagtc aatggttttt atgtcatttt gacctttaat
     7441 ttgaaactat ttacatctta aaaagtcttt ttgacattaa ttgaatggag agtgtttatg
     7501 gatactttag gagaaagaat tgtttatttg agaaaagcaa aaaaccttaa acaatatgaa
     7561 ctagaagaaa tgttaggctg tgacaactta agtaaatttg agagaaacat tagaaaacca
     7621 aattatgaaa tattaaaatc tatagctgag atatttaatg tttcagtaga ctggctatta
     7681 aatggagata atctatcaca taaatcagat ttaatttgcg attcctcctc aaactatcca
     7741 cttaatagca taaactctaa tgaaataaag cttttaaaca actttagaaa gttaaatgat
     7801 tatgataaag ccaaaataga aggtatgatt gaattaaaac ttcatgaata tgaaaaagag
     7861 aaggatttag gaaaaattga gtataacaaa aataaagatg agaaaataga taaataattt
     7921 ttatgttgct tttgatacga ttttacatca tttctttgtt ctaatatttt tatttcattt
     7981 attttctttt ttataatttt atgataaaat aattagtata ttttgcttta cttgaatgta
     8041 attatttata caaaagtcaa acataatata aatgtagtaa atttttagtt tcaagtaata
     8101 tctcgttatt ataactaaat atttattaaa attaggagga taaaatgggc ttaagagaca
     8161 aattcgcgca atcatttgct agatcaaaaa ctatgagtgg ccctgagaaa aaggctaatg
     8221 aaataatggg taaattgctt cttaagaaag ctatattacc tatcgtttta atgttcgtga
     8281 taataatcgc tggagctatg cttaaaatca attcttgggt tacacttggc ataaatttag
     8341 ttattgctgt tggtgctttc ttctacataa gaaattctag taaaaagtat cagaacttta
     8401 aaccttatgt tggcaatttg attagtttag agaaaaaagg taaaaaagag tacgttgcta
     8461 ttataaaaca aggtaagtta ccagttaagt tacaaatagc ttatggtggt gaagaccttg
     8521 aacatgttaa gaaaaatcaa atggttcaga taagttataa tccagatgct aaaatagcta
     8581 ttttggttaa tagacaataa tttaataaaa aaaaggagtt gtcttaaaat tgattttttc
     8641 taatcaaatt taagcttaac tcctttttga atttttatat gttattttta tatattattt
     8701 ttattacttt ttttaattaa tattttaaaa tataaaatcc ttctcaataa aatagggcac
     8761 tatctacaat actactttat cttttcctaa taatatataa tctggtgcta ctgctacttt
     8821 catgttatac accgttttgt aacatacctt ttatatcagt aacatcttcc ttaatttcct
     8881 ctacatctgt tttcattatt cccatttgaa caagtatatt tttgtttatt tcttgttgtt
     8941 gtgtagataa ttctataaaa ttttctacag tttttttata catgtctcta tcttcttttt
     9001 tctcctgcat agtattttta aataatagag cacatagtat acctattgcc cccagactac
     9061 tcaattcggt tattaattta tccattatag cctcctattg tctaaatttt ttatacttat
     9121 agctttaatt tttgttatat tcttacatca tgcaatatta tattttcagg attatcaatt
     9181 taatcttctg tactttaaat tgtaggttta atttatagat tgaataaata ataaatatat
     9241 atacaatttc taatataaca aaaaataagt acaaggctta cttattaaag ttaagacctt
     9301 gtactttaca taaaaaaaat aactaataca tagttattct aaatttacta ttcaactggt
     9361 atttctaatt ctttacatct aattctaact tgctctctaa ttactgctgg tacttcttct
     9421 attgtttttc tgcctcttaa aattaaagct acatatactt ctaccattcc actctcacct
     9481 ccagattttc ttacactaaa agtgcttata ttacttgctt cattgacaga actttgactt
     9541 aacaacatct catatatttc tgcatttgct ataaaattat ttgttatatc atcatcttgc
     9601 tttgcttgac ctatacttat gtctgaaatg tctttatact gcatttctac ttttctacct
     9661 tgtttgattt gttctatttt atttttatcg aatttaaaca atttttacac ctccatttta
     9721 agcttgtggc tcgttgtttt cagcatcata tctaaaaatc atagatggac gttttatagc
     9781 tgtccattta tctccttttt ctactatgat tttaggctga gcacttttta tgttggcttc
     9841 ttctaattca tagatttctt gataacttga gtctgatttt ttgatttcaa ctagtttcat
     9901 gtctttgaat aagtcagatt tatttatttt tacttgaact ttagttttat ttattaattg
     9961 atatataggg tctccaagat ttaaatcttc ttcaagtcct ctatctagtg taattgtaat
    10021 atcttgttca ttagatgttg tattaactac cttgtacatt ctattaccta tcataacata
    10081 agaatcttta attatagaat ttccagtact ctctttaaaa ataaataaag atgcatataa
    10141 atcatcacct ttacaataag ctctaaatag tatcttattc tctcctattt taattttatc
    10201 tggtggtact tcaaaggata ttttttcagt agttaaatca cttacagatt ttacactttc
    10261 accatttata agcatttcta ttctacttat atctttatta gggtcttgta tttttatctt
    10321 aattgatgaa attgattttt cagtaatttc tatagcagca tttgtcgttc ctacttctga
    10381 tataatatat aatgtattgt ctgttgcaag tacatccttt actgtattta catcttcaga
    10441 aataggtact atagtaaatt cttttaaaaa ttctactggt tgttcaattt tatatacatc
    10501 cctatttttt ccagtaaccc atactgaatt atcattttta agtactaata tatggtcact
    10561 tccataagat tttatttctc ttatgtctgt tagtgctttt gtaaagaact taattatagt
    10621 attttcacta tcgcttccta atcctaaagc tcccttatca ttatatcctt gtacaaataa
    10681 ttcattacct gtagttacta ctaatgtata gtttttacca caatgtactt ctttacagct
    10741 agatatagtt gtaattcttt gtaattcagt aactggatta ttgtgaccta tacctaattg
    10801 atattcggaa tttttaccaa tagaccaaag actatcatca gattttataa aataagtaaa
    10861 gttatctcca catgaaacat gctttacatc tgatacatta atctgcacaa attcatccac
    10921 ttgatttata gacaagtctt gcaataattc tccatggtag tttgtaccac atccatacat
    10981 tgttccatca ttaagtaata taataatatg agtcttagaa atagatactt cttttatatc
    11041 ttcatatgga agctcagtat aaaggtctcc tttgtataaa tttcctaata ctaaaccata
    11101 atctatgtct tttgtggtag gtatcactat cttattgttt agaagtataa agttatgtct
    11161 gtttttaata gatgtcaaat cacccattac aaaattcata tgcttataat taaatattct
    11221 attatcatta tttctactat cgtatctatt tccaacacct aattgtccat agttatttaa
    11281 tccacaaata tatatatcat tagtatcttt tattgctata tttgcatatt ctaaaggaag
    11341 tattttcttt actcttgtat tggtttttat aaatgtatct ctttttgtta catcaccaac
    11401 acctaattgt ccattattat taaaacctgt accatatagg tctccattat cacttacata
    11461 atgtccacat ctataattca ttccaatatc ctttgccttt gatgctattt ttttaatctt
    11521 ctctggagtc tcacttcctg tacctgaacc attactacat ttgcctgcag tatatacatc
    11581 cccttcactt gtaagtaaaa gtactgcatt atttctgttt ggagctactt ttactacatt
    11641 tgaaattgga atttcagaaa atgaactttg attgctatta ctatttagac ctaagcaata
    11701 atcagtattt aaacctgtac tatatgcagt attatagatt gttattatat aggtatttat
    11761 ttcatttggt ataacttctt ttacattttc catacacttt ataaaactat actggtcagt
    11821 agtatttccc atacctagtt gtgaattatt attgtatcca caacaatatg caatgtttgt
    11881 gtcctttaca ataaatgttg ttgtatctga gcagtacaca tctcttacat tagttatatt
    11941 gtttacagta aatgtcaata cgtctggatg gttgctttcg cttcctaagc ccagttgccc
    12001 atgagtgttt tctccacatg aatataatgt gttatcatct ttgattgcat gcgagtgagt
    12061 agtccctaaa acaacatctc ttacattttg gatgtttact ttggtaaatg tatttctatt
    12121 tactatatca cctaatccca gttgtccttt ggtatttaat cctgtggagt atagtgttcc
    12181 atcatttcta attgcaaatg ttgtgttacc atctatactt gttgatattt tctttatatt
    12241 atcaaggttt atctgagtga acttatttct atttattgtg tcacctaatc ctaattgtcc
    12301 agaactatta tttcctgtgg cataggctgt tccatcattt tttactataa acattgtatt
    12361 tcttgatgtt gtaatttctt ttatatcatc cataaatata aatgagtttg attgttcagt
    12421 tatatttgtg aattttttta ctggttcgtt gttatattcc cctatcccaa gtttgttatt
    12481 ctgattcatt cctgttacat atatattgtt atttttatct actactacta cagtatttcc
    12541 ttttacaatt atatctttta tgtcttttaa tcctgtatca tagttacttg tgccatctat
    12601 atttgttgaa ttcccaaatt gcccactatt agcaatccct tttccatata acttggaatt
    12661 actatgatat attataaaat tacttcctaa attagaactc tcagaattaa tactagtata
    12721 acttctatgt ccagtaatgg ttgagccaaa tgtttttgga tatccagtaa tatatgaact
    12781 tcctttctta aaaaataata tattatttga catccctatt actttttcaa ttccactaaa
    12841 atcagtctct tgtataaaat aagtattatg atttgaattt gtagcctcaa taaaagttcc
    12901 atatcttgaa taatcagata tacttggact gctatttaat gttcttaaat atccatgata
    12961 tcctgttaaa taaacataac ctccttcgtc aattattgca caaccatcat aaaaaggaaa
    13021 aacattagtt attttaggta acgttctaag tccatttgat gtagaagatg ttgaatttgt
    13081 tctacattga gatactaatg ctttgctttg gttactatta tttcctttac ctaattgata
    13141 attataattg tatccagaaa catatagttt cccttcactg tttatcatat atacagaata
    13201 atcatatgct gctatctttt caatattact tattccctgc ttatatggat aattagaagt
    13261 tttttctata ttattataag gattatttcc ccatccatat acttcttttg agtgatttaa
    13321 agctatgaca taattgcttc ctacacaaaa atcctttaca ttctctattt ctatttttgt
    13381 aaatatattt ctattaactt catctcttaa acccaattgt ccattactat ttaaaccaca
    13441 tgaatataat gttccattat tcttaagcac aaaagttgag tttccgtttc cttttatatc
    13501 caaaacatta tctatatcca ccttagtcat aactgacctg ttaacagtat ctcccaatcc
    13561 taactgtcca taattattta aaccacaagc atacaatgta ccatcattca taacaataaa
    13621 catattgcta tctgttaact ctatcttctt ataatcttga aaattatcac ttaactttac
    13681 atttaaaaat tcactattgt cctttgtaac atataaataa tcatcattaa ccaataaacc
    13741 tgaataatta tctaaatttg aagattgcac atttgaaaac tcatacagaa aagttgtatg
    13801 aaaaaatgaa ccaagttgac ctgctaaatt acagccagat acaaatatct cttttgaacc
    13861 atttattaaa aaattaaatt gagaaccaca tttaatatct tttatatttt ctacattaac
    13921 cttagcaaaa gtatttcttg atgctacatc tttattggca ttggctagtt gaccataatt
    13981 gttttgtcct gtactaaaca gtgtattatc gtattttaat atcatagtat gattatttcc
    14041 acaagaagca tacttaacat tatttatact agaaagagta aatacaattg aattattatt
    14101 atttcctgta ccaagctgac caactccatt atagcctgta gtatacatag ttccatcatt
    14161 cttaataaga attgtatgac tactaccaca cgctatctgt tttacattat ccataccttc
    14221 tattttatta aatacacttt tatttaaagt actattaatt cccaactgcc catttgaatt
    14281 taatccacat gcatgtgctg tcccatccat ttttatgata aatacagatt gattataagt
    14341 cactacatct ttcacactat ctatatttac tttagtaaaa gtagctctat tgttagtatc
    14401 acctagtcca agttgaccac ttgagtttaa acctgctgaa tataatgttc catcattttt
    14461 aagcacaaat gtaaagtcat aaccacatgc aacagttttt acatcatcta ttggtagctt
    14521 agtaaattca taatatactg tagactcaat tacacctaat tgacctacat tacttgttcc
    14581 tgctccatat gcaaatccat ctttagttac tgctacactg tgttttgaac cacaagatac
    14641 atattttaca ttatcaattt ttactttact aaacatcctt ctctctgacg aatcttttcc
    14701 aagtcctagt tgaaaacctg tattatcgcc acaagcatat aaattaccat ctgattttaa
    14761 accaaaggta ctattactta taccagtaaa aaattctaca aaatcataat cattatatct
    14821 tttatcaaaa tcatcaatca atatgttctt atcattaaaa taagcaccac gctgtaaaag
    14881 tttattttgc ttcattatta gtactacctc ctttatttat cttatttcat atgaatattt
    14941 acttagagtt atatcttttt gttcttttaa ataagtgtta ctagattttg tctcaacaaa
    15001 gttattatcc atatcatgta tatatctaac tctatttgca ctaaatggta atactacatc
    15061 tctaaaaatc acattaccaa tttcacctgt tatacatttt ctatttaaat ctaacttaag
    15121 cccttctata gctactatgt tatttccatt tgctaatgta tcataccaat aaccagcctc
    15181 atctacagtt acttctgact ctaattctag gtatattaat atatctagca ctttttcaga
    15241 taatttgtta tagctatttt ttaatactga tatatcagtt tgtatttgac cacttataac
    15301 atcatcaaaa gatatatttt ctattttttt aactacctca tttatagctc ctgataaatc
    15361 tttagaatct gtagttaata tatctgtatc tccaattttt acttttattt cttttatatt
    15421 tttatcaaac ttatcatcta aatcttgaat atccttttga gttacaaaaa ccaacgtcgg
    15481 gtctacttct aatgtaacat ttgaagtatt ggacaattgt aaaatcattt ttacaaccaa
    15541 atctttaata ctgccatctt cagcacgtgg cttatacgtc tctggatact tacctatagc
    15601 tattatatta ttctcattat ccagaacagc agcttctctt atcataaatc caccaactgt
    15661 tcctggtaaa atagtctcta taactatcca attaggattt ttttcatcaa ttcttagaga
    15721 atttatattt ccttcccaaa ctacattctt taaagcagtt tgttcctctg ttggctcata
    15781 atattctcca ttactatctc caacttgaaa cttggttaat ccaacaagct ctcctagtgc
    15841 agttgcattt gctatttttt ctctaccaat tttggttaat attatataaa atttttcttc
    15901 caattatacc acctccgatt taggataaac tactacattt tctacgcctg tatcatttgc
    15961 aatagctata ttaatcttac aattattttc aatgcattta atgcttaaag gataaacctt
    16021 tatttcttct ctacttaaca ttccagctcc acagtttaca acagcactac attttataat
    16081 tggttcaaag tatggatgaa cttttacctc ttcactacat accatacctc caccacaata
    16141 gaccatactt ttatctagta ttattggttc taaatagtgt aataagtgag caggttttac
    16201 tctttcaatc atctcgctac aatctaaaag agttttatag tcacagttat ttgctataaa
    16261 actcaatacg aatgtaaatt catcactata aacttttata tctgtttctg attttgtata
    16321 tgcctcacat atacttttta taacttcaat agtactagta cctctgcttc tcattttagc
    16381 ttttatattg ctacgtcttg tgtcaaaatc aagttctttt ttttcaatgc agagtatgtc
    16441 ttcccacata tctaatcccc atgtcgctgt atcaacaaat aattggttaa aggtatcatc
    16501 aatatcttct ttaagaatat ttaattctgt atcgtatgca ccttgtatct cctctacaat
    16561 gtaatttcta tcaaatgatg gtagtttatc aattagcttc attgattttc cacctcacta
    16621 aaattaacag ttgttacact aggtatttta tcttcattaa tagttatatt atctgtactt
    16681 ccatttataa gtagattact taaatcgtgt acaccagtag tatttataag tattcccatg
    16741 acttttgtat agattatctc tcctctaata tctctaaagt atgtatttat actttctagg
    16801 aaagattctt ttacattgtc taatgtatat ccatcttcta gtttcattac tgcacttata
    16861 cttatttcta ttggtaatgg tgtcacaact gttatagttg gtccaatagg cttctcttca
    16921 tctatatgtt gctgacacct ttcaatcgtt tctgtatcaa cagcttgatt attttcccca
    16981 aatatcaaga ccttaactgt tcctggacca tcccatcttg gataaacctt aacattatag
    17041 actccatcta cttccaaagc ccattcttca tagtgagctt tatttccact tgtagcttga
    17101 tttctttgga ttttataaaa tctttctttt agttcttcat ctgtctctat atctgtacca
    17161 ccttcaaaac ttcttgtgtt agtaatcttt gttactccag atatattatc aactagttta
    17221 aattcacaat ttgtaggtaa attatatttc ttaccaactt ccagagcttg aactgggcta
    17281 ttgtcacctt cttcactacc aatagttaca tcttttatta ctacaaatag taaatctcta
    17341 tatgatatta ttgtgccatt atttattaca gttcctttct ctccaataaa ttcaacttct
    17401 ccatttgact ctgtacctaa ctttctatat acaccaaatt cattgactct tttatcaaga
    17461 aactggttat atgtgtcttg tataaaagcc attttatgta tctttgaaag ttctagatat
    17521 atcttcgaaa gttctagatt atttccagat accatgttgt ttagaaaaga accttctcct
    17581 ttataaatat caagattaat attttcaaga gttctatttt ttattacttc atatgtctgg
    17641 tcactataca tttatttcca cctccccata gatagtggat acttttacat ttgcactcaa
    17701 tacgtctctg ttaaagttag cactttttac gtttacttct aatatatatg gatttattag
    17761 aagggcctct tttatgaatc tactagcttc actttctgta agaccttttg tatatttttg
    17821 tcctattagt tctgaaagct ctgtcccata ttctaaagag tatatctcat gttcatatct
    17881 atttgtcttt atacacttgt atatccaaac ttttaaggct tctttttttt caattatttt
    17941 aaagtcacct ttttctaaaa taggttcatc tttttcaaaa tcccatgcca cttcacgaaa
    18001 gattggcaat tcttctgttt taggtaagat ataatcctct gggacaccta taaaaggaaa
    18061 tattgtactc attataagct caccaactta cttacaacag caaatttttc gcctattcta
    18121 aacattatta ccatatcccc agattcaaag ttatctataa aaggattttt tacttcatgc
    18181 tggtgttctt gatttgtttc tgtgtcaaat gtttctagct gtctatcaat catccaacta
    18241 tctatcaaaa tatcttcttt ttctaatatg atgttatttg tctctatttt taaatctggt
    18301 aatttacttt taatttttcc aataaaaaaa gaaggttgat tatgatactt tccttcttct
    18361 cttattattc ctataaattc atttattgga ttagccatta tatcaccatc cttttataat
    18421 actctcctag ctgtattaaa atctcttcta ctacttaact tacttatttt aactacatca
    18481 cctttttgtg gagcatgtaa aaattctcca ttacctatat ataatccaac atggctgact
    18541 ggattatgaa aaaatactaa atctcctgct tgaagatttt tttgttctac tttcttgcct
    18601 tttttagatt gttgatttga cgttcttggc aaactaacat taactttttt aaaacagtag
    18661 accattaaac cagaacagtc aaaactactt ggtccatttc caccccattt ataaggttta
    18721 cctagatgtt tttttgcctc tgatattact attttagctt tatcagatac attaccagaa
    18781 taaccaccaa taataatttt tccttttctt cttccaaatt tactagcttc ttcaacattt
    18841 ccaaataaaa tatctatatg gtatgttcca tttttttcta tagttattgc aggtcctacg
    18901 tcatttactt tatagactcc atcacgacca gaaactcctg taattacctg tatttcatct
    18961 ccatagttca ataaaggatg tttatttaaa aactcttttg tataataagt ttgctcatat
    19021 ttaccaacca taggagcagc gcaagttttt ttagatgggt caagtttttt ctttctacaa
    19081 tctgtatctc caccttcttc ttttctagga cagtaagctg taaattctgc tgtaaactct
    19141 tttcctcctg cataatcttc tccccctaaa ttactttctt ccttaggttc atcctgtcct
    19201 gctgactttt catccataag attttgaaaa ttaagttcaa gctcaatttg atattctcca
    19261 ttttgccaag tatgtttgtc tgtatctata taaaatagtc ctacaagctt tgtataagaa
    19321 tctttaactt ttactcctct accagttata caacttacat ctccataacc tttaagagaa
    19381 cagctttttt ctatcccatt aaactcgcta tcaatatcta catcttgatt ttcttgttgc
    19441 tgaattactt tttgcattat tacatttact tccttaaaaa tttcattatc tattttttcg
    19501 ctaatcttgc ttccatactg gtctaccact attactttat tttttacatt ttccatgctc
    19561 tccgaaaagg tggtatttat aatattaaat ccctcttcaa acataacact taatgtaaca
    19621 gttccttttt caataacatt aaacttatct aaattagcct ctatcatata ctttttcttt
    19681 gtctttttac ttgcctctgt atatgcactc attattgtgt cataaccatt tactcctata
    19741 aacatctttg tatacttgac attggtcttt gctattgttc caactgaaag cctattttca
    19801 gcaaatactt gctttgctat gtcttcaact aacttatctt taaaattgta tgacacttca
    19861 ctttgtgtaa gtaaaaatcc catatcttta gatacaaaac taatttcatt actgcttgaa
    19921 tctttagacc tattaattat cattcctcta aagagttctt tctcatctac atagaaacag
    19981 actgtactag ctattggtat atcgatttgt tggaaattta catcaaatga tgattgtatt
    20041 attgaaaact ctagtgtcct tgatggagat ttataatcac ctgaccatga taccttgtct
    20101 actatgtcag ttatgtcata tatacttccg ttttttatgt gtacccaaat cttaataata
    20161 aaaaatcacc tgccttttca tttaattcta tcagcagtta cttacaactt attatctata
    20221 atagttattg ttttggagtt acttttggta tctttattct tttatactct tttaaatcta
    20281 atgtaaaata tacatctcga gtcccatctc tttcttcata actaaaccct tcaattatga
    20341 cttccatatt tatatttgtt tccgtaattg taaatcttaa tataaaacct tccttcatcc
    20401 atttttctat cttatttaca caatcatatg gtgatggaaa acctgtatag tcacagaaag
    20461 tgtagtcttt atttggaaaa aaacttgata ttgaaatgct tttaagagct acaccaccaa
    20521 aagttgctac ttcacctatt ttgagtatac tagaagagtt tacagcagct tttccattaa
    20581 tactaaagga agatggaaat actggaaatc taaatctatc ttctgcttgt ctaagccaca
    20641 tttccattat aagacacccc ctaactgtgc caattggata cttgcaacta attttgatgt
    20701 tatcttttct atatcagctt cttctctaat cactattgta tcagctaact tagctatgtt
    20761 tatatttcca ccattttttc tgcttctata ttgatttgct tcttgtttag ttaaaaccat
    20821 ttcaccttca tggagtctgg tttgatagtc attataagga acatagttca atccaaatgc
    20881 attattacct ttcttacttg aataagcttt accagaatca ccagcctttg ttccagcata
    20941 tggattatct ttgtaatttt ttttactctt ttccactaga tttaaaactg ttcctgtgat
    21001 gcttccattt tttatcttat taatcatagt ttgccaccat ttgcatatat ctttaactat
    21061 tttacatact ttatctaata tgttaaaaaa agctcccaat attggctcta taattttcca
    21121 tccagtttcc aacagaggtc ctaaggtctc ccatacagtt ttccaaataa cacccagtgt
    21181 ctctataaaa ttgtttattt gaggtgcata ttcacctaaa aaagtaaaaa ttcttgtgat
    21241 aataggacta acagcttcaa ctacaggtcc gattagttgg aatgctgtag ttactattgt
    21301 accaaatata cttattaatc cttgtgcaat tggtgattta ttgtttatac tatctagtaa
    21361 attatttact aagtttacta ctggaagtag tgaatctatt aatgctgtaa aatcaatatt
    21421 ttgtaatagt gtacttcctg cgttaacaat tgcttttggt gcattcccta tcattgttgc
    21481 tgcatgattt tcaaaacgtt cttgcttatt ttcaaccatc attttacgct tttcagcctc
    21541 attagaaatt cgatattgag tagaatcaga aaactctttt ccatttttta cacttctaga
    21601 ttgcatctgg tcatatttgt tgccaataat tatcttacca ttcattacat tgcctatttt
    21661 accagccatt ttttcagata agccagctaa attccctaat ccactttcta taaaagtttt
    21721 tattttcttt agtccttgac ctaaaatatc atcaaaaggt ttaacaatac tcttcaaacc
    21781 attgttaatg cttgtttgag ctcctgatat tatacctggt attgtattac tcatttcttt
    21841 agctaacccc atctgcttta cacttatgcc ttccaatgct tcatcaaaag ttttataatt
    21901 ttctcctacc agagtattaa gagattccat atttcctttt gctgcactta agaaagctgc
    21961 tgatacatct tgttcactac ttgtattcat atttaaagct ctaacatcta ccatagcttg
    22021 agttatatct ttttcttttt tgccaccagt aatattacta atcattttag ctttagttcc
    22081 cattgcaaca acatcttcag ggtcaaaagg cgtatcattt gccatattta ctaaactgtc
    22141 ataatactta tcaacatctt tttggctagt ttttccagta tttcttttca ttacactttc
    22201 tagaacaact ttctgatttt gttcctgacc aagtaagtca acaccctttt tcacagcgac
    22261 acctatagta gcaccatttg tcaaatcaga tatcttccct tttatcgaac caagtattcc
    22321 acttgccata tcctttacag ctattgcacc actccatacc ttacctgcaa acaatctcaa
    22381 tttgggaaat actttactta taatgctact tgccatatct tttatagcta ttacaccact
    22441 ccaaaaagta tttttaaaca acattaactt aggcaaaatt ctagccatag cactacttac
    22501 catatcttta agattaatac ttccttgata aggacgtttc tctactcgtt ttaattttcc
    22561 caagatacta cttatcactc tcttagtcat atcttttgct tttattacga tattccaaat
    22621 cttgccacca atctttttta aatttccaag taatttattt ttagtttttt ctacttcatc
    22681 acttttagct ttaaatatta atataatcat aatatttgaa ttaaactcat ttttcttttg
    22741 aatcaactta ttaaaataat caataatcat agctatagtt attgctagtc tcataatact
    22801 tccatttaaa cttccaataa ctcctgaaac agcatttatg ctagaactta attttgccat
    22861 agttgaactt acactactag cagtagtttg aacttttctt aacgaggaat ttaaactgtt
    22921 tactctacta cttgcacttc caatagaatt ttttatgcta ctagccatta cattagtgga
    22981 aagtgaaaca gccacagttg ctctcctaaa attatcagtt actgatataa tttggttcat
    23041 ttttggtgtg taattatctg taagatacat atttatacct tcttctctca catttcccat
    23101 tacataccac ctcctgtcaa aggacacact ccatatccat gtttcatttt ttcaacttct
    23161 tcctcaattt ctaaggcgaa aaaagcttca ataagcttta attcgccttt atccattgca
    23221 taaaaaaggg acggtcttat accttttttc ttccaataat agtacattat tgtagtcttg
    23281 gtatccgtcc ctattagttt tttacctctt ctatcactgc atttttacca taacccataa
    23341 catcttgaag tattctatat aggttggtaa tttcacctgg taataatagt tttcttgcca
    23401 attcttttgg tgttggagcc ttaaatttat ccattagttc cttattttta aataaaagct
    23461 ttccatccaa atcacaaaca ccttctataa ttgtaagcat ctgcatctcc ggtaagtcta
    23521 tatccgtttt catattttca tcaattttaa gacaagtatt ttgtatctct tcatctttac
    23581 ttattgttaa agctctacac attactttaa attcctgccc aaatatagtt gataatctct
    23641 taagttcaac tatagtactt ggtctttcta agttttctgt atctgcattt aaaagtaaat
    23701 ctactatgtt tatatttttt gataatccat tttcattcat tattatttgc tcctttatca
    23761 atcaaattca atcaactaac ttttttactt attaactacc taaatttatt aagtatatat
    23821 ctactaaata tcagtataaa accaaatata aaactaaatt atatcaagaa aatcatactc
    23881 agtaaatgtg aaaggtgctt ctatctctcc ttttactcca acctcccaat cagctaaagt
    23941 taaatcatca aatgctatat tttttactgc tattctttct gctccataag aatctgggtc
    24001 atttaattta gatatagcaa caaatctagg ttcagaacct ctctttatct tttctccaat
    24061 taacttgtgc attcttgaac taacatggta tagagttatt gaaccttttc ctttatatcc
    24121 catatacttt gtatcagtac ccatttgacc tgctattata atatcctctt ttgtaaattc
    24181 catctttgct tgaaacttct ttacttctgc tactttgttt ccatcaagcc aaagttctcc
    24241 ccaagtacca ctcattacat ttctagcttc catatttgcc atattaataa tcctcctata
    24301 tttctattga taaatctata tcttccatag catcaagtac ttttattttt gcttttaaaa
    24361 atactttaga acctgtgtta gcttctttta tttcttgtaa tgtcatataa gataaatcta
    24421 ctccttttga ttttaaatac gatttttgtg cttcaaaatc tatttcaaca gtagaatcag
    24481 attctataag tgctgacttt tctaattctt ctaaataact ttttatagct actatcaata
    24541 aacatttgtt gtcataactg ttagtaacct ttcctatata gtcatctatt atcacctttc
    24601 ttatgtcact atgtataata tctaaagtgt caactatttt tattttctgg aacatttctc
    24661 ctttttcttc tgttaactca gttaaagaat ttactcctct tgcaattctt atagcccctg
    24721 cttccttaat aagtataagc tctcctttat taacccttga ttctgcatca actttcgtca
    24781 tcttaggtat atcaactaca tcactaagct tagtataagt tactgattga cttaaaggtg
    24841 ttcctgctat aagtccagcc accctacttg taaactcatc aacactgtat ttcttttctc
    24901 caactaacac atcttctgta gtaaaattaa ttatcccttc atgatttcct acaacttttc
    24961 ctaatacagc tttaacctta accttatcta tatctctaag tttaattatc caatttttta
    25021 tagcagtctt atcagcttct actgcttttg gcatacatag ataattaaat tccttagtct
    25081 ctaaaaaatc taatgcagtt tgaatatctg cttctccttc tattacataa actaataatt
    25141 tatttggagt gttaacattt cccatcaaag ctaaatttat atactcttta ttttcagcag
    25201 ataaatcaac tggtatatcc tctttttcat gtatttcatt aagacctagt gccttagcat
    25261 ctttaagcac cattgcaatt attcctctag ctgaacgttc tttaacagtt gtagctagct
    25321 ccttaaatga tatgttgata cttggtaatc ctatagccat actatcactc tcctaaattt
    25381 aaattgattt cttccatatt ttcatatact acatcttctt catatacact atccagataa
    25441 tttagtgtaa ttgtaaagtt caatacataa attgactctt cttttttaat actttgttct
    25501 agctttgtta tatttaattt tctgtctgtt acctttatat ttctgttgaa taacttattt
    25561 aattcatctg aaatttcata cattctaata ctctttgact gtggttttgg taagtatttt
    25621 atagagataa caatatcttt attattcgta tttaacatta taggtgtaca aataactgat
    25681 agtatactta caaaaaaaca agacccttca gagccttgca cactttcatc atcaataaaa
    25741 atactttcag taaagttttt tttgagaatc tcaataattg tttctaatat ttccttgtat
    25801 ttaagcaaat aatcaccaca ctatcactaa atattcttgc ttaatctgat aatcttacta
    25861 taaatgatat aagtcatttt taaatataca tctcatttat tcctgtcaaa tatcttctat
    25921 atttttaggt aatattattc ttttcaattt atcacctcct tcaatatgga tttctttcac
    25981 aataacatta tctcacatat tttattgcct agtgttgcaa tagtgtatat aaagtgttgt
    26041 aaagttgtca tattttttat aatatctcac tcaatttaaa tatcattttt ttctttaaat
    26101 tgtagcaaga tgttctatct aagtgtagtt tcatagaaat atatgtcata ttgttttttg
    26161 tcttactatt ataaaaaaga ttaaaaaatt ccatttctat atcatttaga catgttagag
    26221 cattttctat cttctctttt tctatttcta tatcttcttt ttccattttt aattttctta
    26281 atctttcctc tctttttaac acctcatttt ccacagaaga acttatgtta tatgtgttac
    26341 tagtcttttc tgtatatacc attgctccac atccagagta ttcattctca catcttttta
    26401 tgtccaaatt aatattcttt atctttatat ccaaatactt ataattatgt aatctttcat
    26461 ctgtttcttt aaataatata tcttttttgt tttccatagt acttctcctt ttttatatac
    26521 ttattattag cttcaaattt acaataatct ctttagtgca cagtttttta ctaatctcac
    26581 aactttcaag aacatattgt tctataactg tataaaaaaa acttcttcta tattttgttc
    26641 taaaatacta gaaatcttct tagccataag aagaccagga ttttttatac ctctttctat
    26701 ttgagatata tagtcatttg taattccagc ctttctggca agttgaattt gtgtcatacc
    26761 tttgcatttt ctataatatt tcaagttatt tttaaacatt gttttatacc tccattacca
    26821 ttaagaatat tttgttcttc cacacttaga ttatatagaa tcatttgttc tttatcaata
    26881 gattttagaa aaaaatattc ttttatattt tattagtttt attttttaga acaatttgtt
    26941 ataatctata cataaggtgg tgttataatg tttgccaaaa gattaagaga acttagaaag
    27001 gaatttggat tgactcaaag agaacttgga gaaaaagtag gcgtttccca aagagtctta
    27061 gggtactatg aaacagaaaa cagatttcct gatgaacaca ttttaaacaa attggctgat
    27121 gtatttaatg tatcagttga ttaccttctt ggaagaacct tagttaagga aaatattgat
    27181 acagtagctg cacatagaaa aaatccacat gaagagttac ctgaagaagc acaagaacaa
    27241 cttaatgatt atatagaatt tttactaaat aaatataaaa aaaaataaac catatgatga
    27301 gcagtctatt tttctgctct tttaatataa tttaaaggca aacatacatt ctattttagg
    27361 gggattttta tgaataattt ggataagctt tttgaattag cttctcaaga agagataata
    27421 attcattata ccacttatat tgcaggtgat ttagaagggc tatatataaa caaacatggt
    27481 attaaaatta tatcattact cagtaattta aaacaaaact caaaaaaatt gacatccatc
    27541 ttagctgaag aacttggaca tcatttcact agccttggat actatgtatc ctcttataac
    27601 gattattata caaaaattat tatagataag tgcgaaaata aagccttaaa atgggcttgt
    27661 gagttcttaa ttactgaaga agatataata aacataatta attctggcat cacttgcgtt
    27721 tatgaaatgg cagacatact taatgtggat attactttct ttcaaaaaag attagaattt
    27781 ttatcattaa aaaaacagtc tttacaactt ggaaataata aatatttaat attaactaat
    27841 ttgccgtatt tctacatatt tgatccaatt tcataataaa aatagtagta attttaactg
    27901 gcttaacagt aaaatctata tatttatttt aacaaatttt ggtttctata aattcaatat
    27961 taatatataa taatgatatc atattattta aaattctaaa aacaaatata tgcttttgtt
    28021 aagaaaggtt gtttgatgaa tgtataaaga aaagttgttt gataattatt ttaagttctt
    28081 agcactatta ttttggccaa ttatgtggta taagtggata gtaatttcaa atggtactct
    28141 tgaaaatatg ttatttacca tatatgcaat cgtcgcaatt attttcatca ttttatattc
    28201 ggtgtttatg ataaaatata aagacattac acaaatagat ttcttttaca ggataagtac
    28261 tctacttgct tttatattta cactttttag ctttttaata tacccaaaga gtttattttt
    28321 cttgtattta aagattattt ttactggaat atacctttat tattcaatag taaaaactct
    28381 taaatttaaa gatgatgaag gtgtagtggg tattatgagc tcacttttat taattgtaat
    28441 aactttgttc tactaaattt aattctattt gacttaatat ggctttaaat attagacatc
    28501 tacattattt tctgatatta tttaagatgg tagtcttata ttaataatga aatacactat
    28561 gaaggagtta actattatgg aaaaaaaagt tttagcaaca gttggagaaa aagagataac
    28621 taatattgat attgaaaatg cattaaaatc attagaccct tatcaagcta tgcaatttaa
    28681 aactgaagaa ggaaaaaaac acttattaaa tgatttagtg aatcaagaat tattcttctt
    28741 agatgctaaa gaagaaaaat tagatgaaga agaaatattt aaattagaaa tgaaaaagat
    28801 agaagaaaat gtattaaagc aatttgctat aaataaagtt ttatctagtg ttaatgtaac
    28861 agaagatgaa aaagtaaaat tctttgaagc taataagagc tctttctcta aaccagaaag
    28921 tgcaactgct aaacatatat tagtagatag tgatgaaaaa gctaaagaaa tacttgctca
    28981 aataaaatca gaagaaatat cttttgaaga tgcagctctt aaacattctt cttgcccatc
    29041 taaagatatg ggtggtgatt taggaacatt cggtagagga caaatggttc ctgaatttga
    29101 agaagctgtg ttctctatgg ctaaaggtga agtaagtgaa ccagttaaaa ctcaatttgg
    29161 ataccatata ataaaattag aagaccttca agaaagtaca gagtctacat ttgatgaagt
    29221 taaggctgaa gttgaaaaaa gtttattata tcaaaaacaa aatgaagttt atggaaataa
    29281 gataaatgct cttaatgcta agtacggaaa ccttgtaaaa tacaacgatt aatcataagg
    29341 tcatatataa aaaagctgtc ttttcacaat atttattaga aaagacagct ttttatatta
    29401 aagtttaaat attcagtttc atattatcat tcttttattg taaagaactc agcttaaaag
    29461 tttaaataag atttttaatt atcattagct aaatttttat atccacgtat tatctcataa
    29521 cagtaaccta ttacatctct tctttttatt atcccaataa atgtgtcttg gtcatctata
    29581 acaggaatga aattttgatt taaagatttt ataactaaat cttcaatatc tgcgttgatg
    29641 gaaacaggag aattatccat ttttctattt atatctgtca ctggaacatc ttccaatccc
    29701 tttaagtcca gatttaaatc gttttttaat gtccataaaa aatcaccttc tgttatagtt
    29761 ccaacatatt taccatcctt acttattatt ggaattgcag agtatttgtg atattccatt
    29821 ttttctagtg cttgccttat agtgtagtct tcatatatat atgctacttc actttttggt
    29881 gttagaaaga ataatatgtt cattacacta cccaccgtta ctgaaaactt ataaatatta
    29941 ctcaatattt ataatgttac ttttttgcag taactttctc ctttcttatt ttctaagtat
    30001 tcttaatact atacgctgta acaagcgttt ttcctttgct cattttaatc ttattatgct
    30061 ttattaatat ttttatatgg tctatcagta tcacttattc tctttttaga aaatttacta
    30121 aaaattaatt gacttaaact tagccataat aattttactc tacactatta attttaacat
    30181 atttatctca ttattaatga ttttatttta ataattttaa tttattttgc tattaatttt
    30241 atttacaaaa actgaaaaaa ccgctaaatg attagcggct tatctctcat attactagaa
    30301 cactaatata tatgaatttt taattataat ctaactacgt tagcagcttg aggaccttta
    30361 gctccatcta ctacttcgaa ctcaacgctt tgtccttctt ctaatgtttt gtatccatct
    30421 ccagttatag ctgagaaatg tacgaaaaca tctccttcac cttctacaga tatgaatcca
    30481 aatccttttt cgttgttaaa ccatttaact acaccagttt tcattaataa atcctcctaa
    30541 aaaaacacaa atatttagct actcttttct gtaaccttat tactactcta tcatatatag
    30601 ctatgtttgt caattattat tatttttata cattaataat caattttttt actttataat
    30661 tttaaatgat tattatcttt tacactttat ttttctttat aattttttaa taatttattt
    30721 ttattatatg gaatcttatg gacttatcac tccagcctaa atcccatggc accaacattc
    30781 tatcagtatt atggcaagtt accaaaggat atcctcttga gtctaatcct gtcactgtcg
    30841 aaacatgtgt tattctgcct ttcttctcat aaccaacaaa atcccccgga cgtaaattat
    30901 aagcttcttt gtatacttct tcataggaac ctttagcaat taatgaacct cttccactat
    30961 acagtgcata atttttaaaa gcttgtgcat ttacccatgc ttttgtacct gctctctcgc
    31021 tataattcca aattgagttc tttttgaacc ttccactttc aaacattatc tgtgatgcaa
    31081 agtttgcaca atctccacct tctggattat aatccctata gtttggatta aatttcatac
    31141 catgttcttc atcagctgcg gctccacagt atttatgtgc ataatctata gccttttttt
    31201 gttcatctgt aagctgtaac tctacaccat cttgttgccc aatatagttt cttatatcct
    31261 cagattttat attttctaaa tttaaagagt ctgcaaaagg gtctgtgtac cactctttag
    31321 ttatgacata ttgattatct ataattttta catttagata atgatatgta cctattctaa
    31381 aggcattttc tttgtccttt tgattttcat atgaatactt atattcagtc gatactgaac
    31441 agattatacc atagaggtct ttttcttttt cttttatttt tttaatctta acccttgtct
    31501 ttatgtcttt aaattttaca ccttgcttac tagaccagtt ttctaaatac ttcatctttt
    31561 ttacttcatt ttcataagcc cacagaccaa attttttatc cgtctcataa agacctttta
    31621 aaatgttttc atttttctct aatattgcct tatttctgta gtcaaataag ttttcaagca
    31681 atagtttata ttggtctttt acatcatcac cttcaacatt tgcagattcc atgacttttt
    31741 cttccatatt tttcatactt aaatcattga ttaaaaatac acctgctgta gtagctaaag
    31801 acaaaaatac aaatgccatt atacctttga tgtacttatt cttatttttc agtattttca
    31861 aagctacacc tcccaaatag tacaccttat aaatgaaatt acaaagaaga ttctgtcata
    31921 tacaataatt ttcatattta ataattctct attttattat gtccaatatt aaaaattaat
    31981 tgtatcaata cactgttttt tttacaattt tttattttat aatctacttt tttctttttt
    32041 ttatagtaat atatatgtat ataaatattt tggtatcatt ataattaata catactaatg
    32101 gagggttttt tatgcaaaaa aaagtcgcag caattaatga cttatctgga ataggaaaat
    32161 gctcattatc ggttgctata cctatacttt cagcattaaa ggttcaatgt tgtccatttc
    32221 caacggccat actttcaagt caaactggtt atcctgaatt tactttctta gactttactg
    32281 atgaaatggt aaaatattca aatgtttgga aaaatttaaa agtcaatttt gatagtatat
    32341 atagtggttt tttgggttca aagcatcaaa ttgagatagt ggctaatttt ataaatgatt
    32401 atcccaatgc ttttatcgta gttgaccctg tgatgggtga taatggagtt atgtatccaa
    32461 tattcacaga agaaatgaga caggaaatta aagaattagt taaacattca gatttaacta
    32521 ctcctaattt aacagaagct tgtttcttaa ctggaaatga ttatactaaa agcgattata
    32581 atagagatga gcttatatat attgcaaaat ctgtatctga tttgggacct agcaaagtcg
    32641 ttattactgg tatattagaa gatgataata tattaaattt ggcttatgat agagataatg
    32701 accatgtatt ctttacatca gtcaaataca ataattgttc ttacagtgga actggagata
    32761 tattcacatc tatactttgt ggtatgttag taaataaaca tgatttggga gtagcggtta
    32821 atactgcaac agattttata tataaaacta taaattacac atctcaattt gatactgata
    32881 gaaatgatgg agttatgttt gaaaactttt tatcagattt aactaatata taaaatcaaa
    32941 aaaactatcc attttataaa tacctataaa tcataaaatt taggtattta caaaatggat
    33001 attatttact aaactattgg tatttcaatt gttaccatag caccaccagt tttcacagaa
    33061 ttttcaatat ataaaattcc attatgtttt tgtacaatat tatttacaat ataaagtccc
    33121 attccgtaat gagatttaga atttctactc aaatctcctg tataaaactg ttctgtagct
    33181 tttgacaaat ctgctttaga aaaaccactt ccacaatctg taactacaaa ttttaagtat
    33241 ttattactta ctgatacact tatgtatagt ttactctggc ttggtgaata atctatagca
    33301 tttgaaatta tattcataat agctctgaat aacagttctc tatctatagt tatattttta
    33361 ggcaaattat cttgtttata aaactccaca tctaagtttt tagtactagc taatgcttct
    33421 atttgcagta agacatcttt tataaactca ctcatattaa ttgcttcttt atttaatata
    33481 tagccattct ctgtttttga tatatctatt aaagtattaa tatatttttc tatttgagtg
    33541 acaccctttg agatatattc catgtattct aataactcag gattttcatt tatttcaatt
    33601 aataagtcag tatttccatg tataattgtt aatggtgttt ttatatcatg tgccagtgca
    33661 gatatctgtt cctttctatt ttcttctagc atccattgtt tttctaaaga attctttaac
    33721 gccaacttca ttttatccat agaaaaaagg atattgttaa tttcataaat tttactagat
    33781 tctacaacaa aatctaaatc ctgttgttct attttttctg tagtattttt taaaagctcc
    33841 atttcaatat taaatttctt gccaaatact tttgataata taacaatttc aataaaaaat
    33901 attatactaa aaataatcat acctaacatc tctggttttg gtaaatatgc tcttaaaact
    33961 ggagaactat actctgatac tagtttgtat tttattataa aaacttcatc tttcttaaaa
    34021 aactttatat aactttctga atatgcatgc ctttcttcaa tatctctcat taaattccat
    34081 acttgttttg attctttttt attaaaagtg cctgagatca aattcccatc taaagtataa
    34141 acaccatact cgtataaatt tggaattata tcttcaattg ctttttcact tgaaataatc
    34201 ttttctttat atttatcaaa ttgtttttct gaataattag ctggtaatat tacacctgat
    34261 gataataata cagaaaatga taaca
//
LOCUS       DAEPLF010000021        33406 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEPLF010000021 DAEPLF010000000
VERSION     DAEPLF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33406
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.021"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            35..1087
                     /gene="ytvI"
                     /locus_tag="KR314_000895"
     CDS             35..1087
                     /gene="ytvI"
                     /locus_tag="KR314_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBH0327294.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            1178..2404
                     /gene="pepT"
                     /locus_tag="KR314_000896"
     CDS             1178..2404
                     /gene="pepT"
                     /locus_tag="KR314_000896"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBH0327295.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            2611..3663
                     /locus_tag="KR314_000897"
     CDS             2611..3663
                     /locus_tag="KR314_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBH0327296.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(4081..4650)
                     /gene="yfcE"
                     /locus_tag="KR314_000898"
     CDS             complement(4081..4650)
                     /gene="yfcE"
                     /locus_tag="KR314_000898"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBH0327297.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(4675..5475)
                     /locus_tag="KR314_000899"
     CDS             complement(4675..5475)
                     /locus_tag="KR314_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBH0327298.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            5610..6482
                     /locus_tag="KR314_000900"
     CDS             5610..6482
                     /locus_tag="KR314_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327299.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDREKFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVRGCSI"
     gene            6470..7156
                     /locus_tag="KR314_000901"
     CDS             6470..7156
                     /locus_tag="KR314_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBH0327300.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            7208..7750
                     /locus_tag="KR314_000902"
     CDS             7208..7750
                     /locus_tag="KR314_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327301.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDIGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITESTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            7829..8296
                     /locus_tag="KR314_000903"
     CDS             7829..8296
                     /locus_tag="KR314_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBH0327302.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKSEKWVDVCKNW"
     gene            9004..10140
                     /locus_tag="KR314_000904"
     CDS             9004..10140
                     /locus_tag="KR314_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBH0327303.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLKPLASGDKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            10199..10981
                     /locus_tag="KR314_000905"
     CDS             10199..10981
                     /locus_tag="KR314_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH0327304.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            11000..12010
                     /locus_tag="KR314_000906"
     CDS             11000..12010
                     /locus_tag="KR314_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBH0327305.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            12029..12826
                     /locus_tag="KR314_000907"
     CDS             12029..12826
                     /locus_tag="KR314_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBH0327306.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            12924..13769
                     /locus_tag="KR314_000908"
     CDS             12924..13769
                     /locus_tag="KR314_000908"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBH0327307.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            13856..15031
                     /locus_tag="KR314_000909"
     CDS             13856..15031
                     /locus_tag="KR314_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBH0327308.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            15226..16128
                     /locus_tag="KR314_000910"
     CDS             15226..16128
                     /locus_tag="KR314_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBH0327309.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKKEK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            16142..17146
                     /locus_tag="KR314_000911"
     CDS             16142..17146
                     /locus_tag="KR314_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBH0327310.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(17290..17517)
                     /gene="acpP"
                     /locus_tag="KR314_000912"
     CDS             complement(17290..17517)
                     /gene="acpP"
                     /locus_tag="KR314_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBH0327311.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            17689..18114
                     /locus_tag="KR314_000913"
     CDS             17689..18114
                     /locus_tag="KR314_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327312.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            18162..18368
                     /locus_tag="KR314_000914"
     CDS             18162..18368
                     /locus_tag="KR314_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327313.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            18550..18729
                     /locus_tag="KR314_000915"
     CDS             18550..18729
                     /locus_tag="KR314_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327314.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            18763..18963
                     /locus_tag="KR314_000916"
     CDS             18763..18963
                     /locus_tag="KR314_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327315.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            19216..20232
                     /gene="ccpA"
                     /locus_tag="KR314_000917"
     CDS             19216..20232
                     /gene="ccpA"
                     /locus_tag="KR314_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBH0327316.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(20355..20753)
                     /locus_tag="KR314_000918"
     CDS             complement(20355..20753)
                     /locus_tag="KR314_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327317.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGGQQKKSKGLFKKTKKSNPHE"
     gene            20965..21870
                     /locus_tag="KR314_000919"
     CDS             20965..21870
                     /locus_tag="KR314_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327318.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            21985..22165
                     /gene="ssrS"
                     /locus_tag="KR314_000920"
     ncRNA           21985..22165
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KR314_000920"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            22348..23565
                     /gene="cdeC"
                     /locus_tag="KR314_000921"
     CDS             22348..23565
                     /gene="cdeC"
                     /locus_tag="KR314_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBH0327319.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDECNPCNPCNPCKPNPCNPCKPNPCDDKCECHDKCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSIPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(23666..24988)
                     /locus_tag="KR314_000922"
     CDS             complement(23666..24988)
                     /locus_tag="KR314_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBH0327320.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(25039..25833)
                     /locus_tag="KR314_000923"
     CDS             complement(25039..25833)
                     /locus_tag="KR314_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBH0327321.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQYEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            25943..27388
                     /locus_tag="KR314_000924"
     CDS             25943..27388
                     /locus_tag="KR314_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0327322.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSSNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTNKYKLSKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISVISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            27417..27626
                     /locus_tag="KR314_000925"
     CDS             27417..27626
                     /locus_tag="KR314_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBH0327323.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            27646..29040
                     /locus_tag="KR314_000926"
     CDS             27646..29040
                     /locus_tag="KR314_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBH0327324.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTALPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGFDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            29280..30032
                     /locus_tag="KR314_000927"
     CDS             29280..30032
                     /locus_tag="KR314_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0327325.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDYLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            30058..30471
                     /locus_tag="KR314_000928"
     CDS             30058..30471
                     /locus_tag="KR314_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBH0327326.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDIECEYIILSDLYGASVCTAMLRLTNLHNVKLFSGMNLNMVLELLTR
                     FSDELTVEDIEQLVEESRRGIQSVTLQIKEEEEVF"
     gene            30475..31218
                     /locus_tag="KR314_000929"
     CDS             30475..31218
                     /locus_tag="KR314_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBH0327327.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLELGDMAIIGSYF
                     KDNYKDFGNVSVEHVKTFMDEIKKIREEL"
     gene            31218..31688
                     /locus_tag="KR314_000930"
     CDS             31218..31688
                     /locus_tag="KR314_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0327328.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDELLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            31705..32463
                     /locus_tag="KR314_000931"
     CDS             31705..32463
                     /locus_tag="KR314_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0327329.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            32447..33289
                     /locus_tag="KR314_000932"
     CDS             32447..33289
                     /locus_tag="KR314_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0327330.1"
                     /translation="MTTSSKKLDTISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSSSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGIV"
BASE COUNT        13070 a         3646 c         5993 g        10697 t
ORIGIN      
        1 aaaaatataa attagaagaa aggagatttt taagatgagc atactagaaa ataatttttt
       61 atacaaatta aaaaacaaca ctatcttttt ttgtatatat acattgttat ttatttttat
      121 ttacaaagcg tttccataca tagctccatt ttttctaggt gctataatag cacttatgat
      181 taatcctata tcacaaaagt tagaaaataa gtttcgtata aataaaggta tctctactct
      241 tgttctaagt tttcttgcag tggctatagt cagtacagtt actacaataa tagtaataaa
      301 ttcaatgaaa gaattaatgg gatttttaaa taatatatct gctaatcctg aggatataag
      361 taatactatt atgtattttt taaataaaat aaatgacttt atgaaatcat ttcaagaaat
      421 agccaatttt gatgtagaac aattagtaaa caaattcagt ggtgaagtaa tgcaaattac
      481 aaaaaatctt cttactagta tacttggatt ggctacttct attccatata ttataatatt
      541 tataattacc ttgtttattg ctacctactt tatagcaaaa gaccttgata agattgaaaa
      601 tagtttttat aatatgttta ctgttgatgt tagaaagaag gtaaaaaacg taaagaaaga
      661 ggcagggctt tcacttgttg gatatataag ggcctataca atacttatgg ctataacatt
      721 ttttgcaatc tggggaagtt ttgctttatt tggattgaaa tacggtctaa tagtagggtt
      781 tgtaggagct ttaatggatt taattccatt cctaggtata ataacaatat atcttccagt
      841 aattgtttat tattttctta taaaaaatta ttttatagct ataagtatga ctgtgatatt
      901 ttttgttttg tctttgatta gagaaatact tgaacctaaa ctagtatcag taaatgttgg
      961 actaaatcca ttagctaccc tagcagcgat ttttattgga atccaagtaa aaggaataat
     1021 tggagtaatt ttttgtttgg gtttggtatg tatgcatgat atacttaaga aggtggatat
     1081 tttttaatta taataattga tagatttttt taaaaataaa attttattca caggtaaata
     1141 aaaaaatatt aatcttatta agaaagaggg tatatacgtg aaacaaaaag tagtggaaag
     1201 atttttaaaa tatgtatcat ttgatacaac atctaattct cagtgtgaga actgtccatc
     1261 ttcagaaggt caaagagttt tagctaagta tatagttgaa gagctaaaaa caatgggtgt
     1321 tgatgatgtg agtttagatg aaaatagtta tataatggct actttaaaag gaaatactga
     1381 tggtgtggat actataggat tcatatcaca tttagataca attgaagatg ttagtggaaa
     1441 agatattaaa ccaagaataa tagaaaatta tgacggaaaa gacatagttt taaatgaagc
     1501 gttaaatgtt attacgtatg taaaggattc tccagagttg gaagaattta aaggtgacga
     1561 tttaatagtc actgatggaa caactctact tggctctgat gataaagctg gtatagctga
     1621 gatagtgact gcaatagagt atctaataaa tcatccagaa attaagcatg gagatataaa
     1681 aataggattt acaccagatg aagaaattgg tagaggtgct gatttatttg atgttgaaaa
     1741 atttggagca aaatatgcat atactttaga tggaggaata gttggagaac ttgaatgtga
     1801 aaattttaat gctgcaaatg caactattac tatacatggt agaaatgttc atccaggttc
     1861 agctaaaaat aaaatggtaa atgctataca tatagctgct gaaatatcag aaatgttccc
     1921 agcagatgaa agaccagaaa caacagaagg atatgaagga ttttggcatt taaattctat
     1981 aggtggaaat gtcgagaatg taagtatggc ttatattatt agagaccatt gtagagaaaa
     2041 atttgaaaat agaaaatcta ttatgataga gaatatagaa aaaataaata agaaatacga
     2101 taatagagtg gaattggatt taaaagattc ttattacaat atgaaagaaa agattgagcc
     2161 agttatgttt atagttgata tagctaaaga agctatggag gaattaggta taaaaccacg
     2221 tttagttcct gtaagaggtg gtactgatgg agcaagatta tcttttaatg gattaccttg
     2281 tccaaatata tttactggtg gattaaactt ccatgggaaa aatgagtgta taccagttag
     2341 ttctatggag aaagctacta aactaattgt aagaatagct gaaaaatatg ctgaaagagt
     2401 ttagttaaaa agtatttaaa aattaagcat ttaaagttat aatagctata tcattcttta
     2461 taagtcaagc tgattataag cttgtgttgt atttaaaatg gtatagctat ttttgtaatt
     2521 atttttataa aagataatta tttttagttt agggtatcaa taaaaagtat ggatatcaat
     2581 aggtattaac atttaaaagg gggaattgta ttgaaaatta aatctaaaaa aataatagca
     2641 tttgcacttt cacttgggat tatgttgcca agttgtgtat atgcagacac tattagtaaa
     2701 aatcaaaatg cacaggtaga tatgaacaat tatcttcaag gtagaaaatc tccaaaggag
     2761 gaaaaactta ttggtgaaga tagatttgat acagcgataa aaatatctca aagtggttgg
     2821 aataatggtt cagaaagggt gtttctagtt aactcaaatt cattgccaga tgcattggct
     2881 tcaactcctt tggcaagtaa attggatgca ccaatactgc ttacaaataa aaatagtata
     2941 ccatataatg taacagagga aataaaaagg cttaatccag cagaagttat tttaattgga
     3001 agtgaaggag ctatttcatc aagtgtaaaa gaatctcttg aagatatgga agtattagtc
     3061 agtagaatcg gtggagcaga tagagaagaa acatctttac ttcttactag acagctagat
     3121 gatactggtg atttaggtgt ctcaaaagtg gcagtggtta atgggtataa tggactggca
     3181 gatgcaactt caatatcaag cccagctgca agtgataata cagctataat atatacaggt
     3241 aaggatagca taagaagtga ggcaaagaat tttattacac aaaactcaac taagacatac
     3301 atagtaggtg gggagtacag tataagtaaa aaacttgaag gacagttagt aaactcagaa
     3361 agactagcag gaacagatag aaaagatact aatgcgaaag ttttagaaaa gttttatgga
     3421 aaatcaagta aggttaataa tatgtatttt gcaaaagatg gaagtggtag agaagctgat
     3481 ttagttgatg ggcttgctgc tggagtatta gcatcaaaaa caaagtcacc tgtagtactt
     3541 gcaagtggaa gtttaagtag tgcacagaaa agttttataa aaaaagtaaa ggctgataaa
     3601 tttgtacaag ttggtggagg taaaaatagt aaaccatatg cagaagcatt agccttacag
     3661 taatattgtg taaatcaaaa ttaatatata ataatagcaa gtaaaccaag ttgaacttca
     3721 atatatataa taaaataagt aaactaagtt atatttcagt ataatatata atgaaataag
     3781 taaactaagc tatgtttaaa tataatatat aataaaataa gtaaactaag ttatatttca
     3841 gtataatata taatgaaata agtaaactaa gttatactcc aatataatat ataatagaaa
     3901 caagtaaact aagttatatt tcaatatagc atataataaa acaagtaaac cagattctat
     3961 ttaattagaa gtttaaggta ttgtacttaa acttctaatt gagatagaaa taaaatggtt
     4021 tacttgtttt tattatgagt ttaataaatt aaagcttatt ttatttttat taaaaattta
     4081 ctaatcattg atatttatta tatttccagt attgatgtca attaagttta tatcaagttc
     4141 aagctcatcc tcatcatcag tttttatgat atctataatt gcgacagaat ggcttccatc
     4201 ttttgggata gaagtacttc ctggatttat tactattaaa ttatcatcca tgtatagttt
     4261 ttttacatga gtatgtccta aaactaatat gtcaccaccc attttctttg ctttgtcaat
     4321 aatttcttct tctgattcta tataaccatg attcaatata attctaattt caccaaattg
     4381 gctcatgaca taaggactct gaataggatg ttctataacc atctggtcaa catctgcatc
     4441 acagttccct tttacaatca caatgttttc taatttattt aatgtctcaa taaatttttt
     4501 aggattatat ccttctggaa tatcatttct aggtccatga tataagacat ctccaccatg
     4561 taataaaaca tcacaatcag ataatacatt taacgctttt tcaaagtaaa gtaagctacc
     4621 atgagtatca ctcattattc ctattttcat attaaaaatc ctcctaagta cagtctataa
     4681 aaatttttca gatactctat gccaaaactc atagtttgac attctaagta attttatttt
     4741 tgcatcagat atactaactt ttatattttc gatttgtcta aatctgtgct caactccatc
     4801 aacgacaatc aatacagaat cttcaaatct atattctggt gcaatcttta taacagattc
     4861 atttgaacat attatacttg atgtaaagca cctatatgca ttggtattta tcggatgtaa
     4921 aggtgtaatc tgcataagtt caagatttat atcaacaata ctacctccag cagaatagtt
     4981 ataggctgtt gaacctgtgg aagtagaaat aatcatacca tctccactaa aattttgtat
     5041 atgtttatta tcaaggctta aatttaggtg tatagtccta gacttgtctc ctcttataac
     5101 aacctcattt acagcaagca tattagaacc actagtagtt gtatatactt ccgcattaag
     5161 aagtgacatt tcttgaataa tataatcttt ttttgtataa gcttcaataa agctatcaat
     5221 cttatctggt aaaatatcag gaaaaaaccc cagatgacct gtatttattc ccattatggg
     5281 aatctcagga aaatcgaaat ctctaactgt tcttaaaaaa gagccatcac caccaattga
     5341 tataataagc tctgtgtctg gataaatatc tgaatacact tcaaagccag cattgataag
     5401 ttttctagtt aagagttctt tagtctcaag ggacttattt aattggtttg tatttatagt
     5461 tataattctt ttcatatatt cacctcttca tactttgtat tataacatag ttttataatt
     5521 cttaatttat aaatttataa atgacctttg tagtgaatgt gttaaaatat gtataggtgg
     5581 gtataaaaaa atgcataaga ggtgaagatg tgtttagcat aaggaatatc acaaaaaaat
     5641 taggaaaatt caagttaaat aatataaact tagagttaaa ggaaggggat attgttggga
     5701 ttataggacc aaatggttcg ggaaaaacga ctttaataaa aattataatg gggattattg
     5761 atgcagatga aggtgaaatt gaactttgta atgaaactat tgaaaattct ccaataagct
     5821 ttaaaaacaa tattggtttt gtatatgatt ctttacaatt ttatccacat ttaaaggtta
     5881 aggaatttag aaaaatagtt tctttatttt ataagaattt tgatagagaa aaatttgatg
     5941 aataccttaa taagtttgac atagaagaaa atatgcatat tgaaaattta tctaaaggtc
     6001 aatctgagaa gcttatgtta tcaagcgcac ttagtcataa tgctaagtta ttgattttgg
     6061 atgaaccaac tgctggtata gacccgattg taagaactga aataatgcag tacttacaag
     6121 attttgtgaa aaatggaagt agtagtgtaa taatttctac tcataatact gataatttaa
     6181 ttaaaattgc tgattatctt gtttttataa atagaggaaa tcagattttt actgttaaga
     6241 aagaattaat agaacaagaa tataagataa ttagggcaaa taaagcagag ttagaagcaa
     6301 ttaaagaaag tatagttggt gttaaagagt ataaatatta taatgaggca cttgttaaag
     6361 ttggagatag cttgaaagta aaaagcctat tgatagaaat tgataagcat aaggtcaaaa
     6421 atcctactat agaagaacta atgtattatt atgtaaatga ggtgagagga tgttcaattt
     6481 aataagaaaa gatttaataa taggaattag ttcagatggt atccgtaatt taaagtacat
     6541 attgctattt tttgtgtttt attttttcct taattcaatt tcatattata cagtaagtat
     6601 tgttgttagt tatttgatat ttataaatac atttgagtgt gattatgaaa atgattcaag
     6661 gatttttata aggagtatgc cagttagtat agaagatata gtttactcaa aatatttatt
     6721 aggtgtagga ctaataattt ctgtaacaat aattgtgtcc ttactaagta aattgacatc
     6781 actagtgttc tttagaaata tggttttaaa tgatgtattt ttttctgtaa atatattttt
     6841 agcaattttg acgattttac tgcctttaat ttttaagttt ggatatggaa aaatgaaagt
     6901 ttgtggtttt atagtaagta ttttaattta ctttgtatat ggaagtcttt tgaagatgat
     6961 tagtatgatt gtatatcaag taaagcattt taattatagt aaagttggag gagtatatct
     7021 ttcaaattat gtaactgata tagctaatac taagtatata aatttatata gtatgacatt
     7081 tttaacaata ataattttta taatatcaat gtatttttct attaagattt caaaaaagaa
     7141 taagtttaat tattaaaatt ttaattaata ttatagcaca atagcacaaa atataaggag
     7201 actaaaaatg aagaagttta taaaagtttc attttttatt ttggggattt ttacactatt
     7261 catagctata aataaattag tatatagaga tatggtaagt gtcaaagatt atgaaataaa
     7321 tcatttagag gaaattgttt ttgattttga tagcaggaga agtagagata tcggtactat
     7381 tgtaagattt aaaataagca ataatagtaa atatagttat aaattaaaca gtgctaaaat
     7441 gaagtttgaa aattgtgtgg aaaataaaaa gggaatgaat aagtgttctg catatgtaaa
     7501 tgtagattta tttaatgaaa aaaattctat aactgaaagt actctgaaat atggaataaa
     7561 accaaataat caaggttatg ttgattttgt aattccaaaa ggactaaatt ttgattatag
     7621 gtactttaat gaatctggta tgagtgtaga atataaagga gaatatattg tcaatattcc
     7681 aatggcaaaa ggactttatc tagtagttgg aaaaaataat gatacatgga atgctaatat
     7741 tatggaataa tttgaaaaat aaatcaatta tagatataat taagaataat atgaaattta
     7801 taaaaattca gactaggagg ggtatttaat gtcaataagt aatgtaagag agtattttaa
     7861 acaatttggc aaggaagaca gtatattaga atttgaacaa tctagtgcta ctgtggaact
     7921 tgcagctgaa gcagctgggg ttattccagc aagaatagct aagacactct catttaaaat
     7981 aggagatgat gcaattttaa ttgtgacagc tggtgatgct aagattgata ataagaagta
     8041 taaggctgaa tttaattgta aggctaaaat gttgacacca gaagaagtct tggagtttac
     8101 tggacatgct attggtggag tctgtccatt tggtttaaaa aattctataa aagtctattt
     8161 agatgattca atgaagagat ttgatacagt atttcctgca tgtggcagta gtaactctgc
     8221 aatagaactt acatgtgaag aaatggagaa gttttctaag agtgaaaaat gggtagatgt
     8281 ttgtaagaat tggtaaaaaa atatagtaaa ctatagtagg aattagtaaa aaactacctc
     8341 tgtatacaat ctatacagag gtagtttttt gtgtaaatat ttataaatct aagaggaaaa
     8401 tctaatttaa actaataatc caaaataaag ttaaaaaatt ctgatttttt ttcgaaacaa
     8461 taaaaaaatg acaaaaaagt ggaaaaaatg taataaatat tgttaaaaaa ataattagaa
     8521 attattgaaa aattatcctt tgagtgttac tattatagat gtaagatatt taaaaataaa
     8581 aaatagtcag ataaaaatac gattatttag ctctattaaa ataattttta tacgataaaa
     8641 atcttctgta catggctaaa aaacagatgt acagacaata tttaatgttt aatataccct
     8701 aatataaata tcgtaaacaa attattttat ctgtgaccag tgtgaaagaa atcttattct
     8761 aacttgaaat ttcacaaaat tccaaaaagc gagatttttt ttaagtatag ttgattttaa
     8821 attaacaatt tatgattaaa ttaatgtcat aattggttag tataatcaca tataaatata
     8881 gagcgtagtt tgattaaggt tataaatttc tcgacgtata ttcaaatacc ttattataga
     8941 tgttgccgag attttaatta ataaatgaaa ataaaatatt tataaaatac tgaggaggct
     9001 attatggatt taaattctaa aaaatatcag atgcttaaag agctatatgt aagcttcgct
     9061 gaaaatgaag ttaaaccttt agcaacagaa cttgatgaag aagaaagatt tccttatgaa
     9121 acagtggaaa aaatggcaaa agcaggaatg atgggtatac catatccaaa agaatatggt
     9181 ggagaaggtg gagacactgt aggatatata atggcagttg aagaattgtc tagagtttgt
     9241 ggtactacag gagttatatt atcagctcat acatctcttg gctcatggcc tatatatcaa
     9301 tatggtaatg aagaacaaaa acaaaaattc ttaaaaccac tagcaagtgg agataaatta
     9361 ggagcatttg gtcttactga gcctaatgct ggtacagatg cgtctggcca acaaacaact
     9421 gctgttttag acggggatga atacatactt aatggctcaa aaatatttat aacaaatgca
     9481 atagctggtg acatatatgt agtaatggca atgactgata aatctaaggg gaacaaagga
     9541 atatcagcat ttatagttga aaaaggaact cctgggttta gctttggagt taaagaaaag
     9601 aaaatgggta taagaggttc agctactagt gaattaatat ttgaggattg cagaatacct
     9661 aaagaaaatt tacttggaaa agaaggtcaa ggatttaaga tagcaatgtc tactcttgat
     9721 ggtggtagaa ttggtatagc agcacaagct ttaggtttag cacaaggtgc tcttgatgaa
     9781 actgttaaat atgtaaaaga aagagtacaa tttggtagac cattatcaaa attccaaaat
     9841 acccaattcc aattagctga tatggaagtt aaagtacaag cggctagaca ccttgtatat
     9901 caagcagcta taaataaaga cttaggaaaa ccttatggag tagaagcagc aatggcaaaa
     9961 ttatttgcag ctgaaacagc tatggaagtt actacgaaag ctgtacaact tcatggagga
    10021 tatggataca ctcgtgacta tccagtagaa agaatgatga gagatgctaa gataactgaa
    10081 atatatgaag gaactagtga agttcaaaga atggttattt caggaaaatt attaaaatag
    10141 ataaatatta aaatatacac aataggtcga tattaaaata gatatggagg aaggatttat
    10201 gaatatagtc gtttgtataa aacaagttcc agatacaaca gaagttaaac tagatcctaa
    10261 tacaggtact ttaattagag atggagtacc aagtataata aaccctgatg ataaagcagg
    10321 tttagaagaa gctataaaat taaaagaaga aatgggtgct catgtaactg ttataacaat
    10381 gggacctcct caagcagata tggctttaaa agaagcttta gcaatgggtg cagatagagg
    10441 tatattatta acagatagag catttgcagg tgctgatact tgggcaactt catcagcatt
    10501 agcaggagca ttaaaaaata tagattttga tattataata gctggaagac aggcgataga
    10561 tggagatact gcacaagttg gacctcaaat agctgaacat ttaaatcttc catcaataac
    10621 atatgctgaa gaaataaaaa ctgaaggtga atatgtatta gtaaaaagac aatttgaaga
    10681 ttgttgccat gacttaaaag ttaaaatgcc atgccttata acaactctta aagatatgaa
    10741 cacaccaaga tacatgaaag ttggaagaat atatgatgct ttcgaaaatg atgtagtaga
    10801 aacatggact gtaaaagata tagaagttga cccttctaat ttaggtctta aaggttctcc
    10861 aactagtgta tttaaatcat ttacaaaatc agttaaacca gctggtacaa tatacaatga
    10921 agatgcgaaa acatcagctg gaattatcat agataaatta aaagagaagt atatcatata
    10981 atttaagggg ggataaagaa tgggtaacgt tttagtagta atagaacaaa gagaaaatgt
    11041 aattcaaact gtttctttag aattactagg aaaggctaca gaaatagcaa aagattatga
    11101 tacaaaagtt tctgcattac ttttaggtag taaggtagaa ggtttaatag atacattagc
    11161 acactatggt gcagatgagg taatagtagt agatgatgaa gctttagcag tgtatacaac
    11221 tgaaccatat acaaaagcag cttatgaagc aataaaagca gctgacccta tagttgtatt
    11281 atttggtgca acttcaatag gtagagattt agcgcctaga gtttctgcta gaatacatac
    11341 aggtcttact gctgactgta caggtcttgc agtagctgaa gatacaaaat tattattaat
    11401 gacaagacct gcctttggtg gaaatataat ggcaacaata gtttgtaaag atttcagacc
    11461 tcaaatgtct acagttagac caggggttat gaagaaaaat gaacctgatg aaactaaaga
    11521 agctgtaatt aaccgtttca aggtagaatt taatgatgct gataaattag ttcaagttgt
    11581 acaagtaata aaagaagcta aaaaacaagt taaaatagaa gatgctaaga tattagtttc
    11641 tgctggacgt ggaatgggtg gaaaagaaaa cttagacata ctttatgaat tagctgaaat
    11701 tataggtgga gaagtttctg gttctcgtgc cactatagat gcaggttggt tagataaagc
    11761 aagacaagtt ggtcaaactg gtaaaactgt aagaccagac ctttatatag catgtggtat
    11821 atctggagca atacaacata tagctggtat ggaagatgct gagtttatag ttgctataaa
    11881 taaaaatcca gaagctccaa tatttaaata tgctgatgtt ggtatagttg gagatgttca
    11941 taaagtgctt ccagaactta tcagtcagtt aagtgttgca aaagaaaaag gtgaagtttt
    12001 agctaactaa tttcttgggg agggtattat gagtacaagt gatgttaaag tttatgagaa
    12061 tgtagctgtt gaagtagatg gaaatatatg tacagtgaaa atgaatagac ctaaagccct
    12121 taatgcaata aattcaaaga ctttagaaga actttatgaa gtatttgtag atattaataa
    12181 tgatgaaact attgatgttg taatattgac aggggaagga aaggcatttg tagctggagc
    12241 agatattgca tacatgaaag atttagatgc tgtagctgct aaagatttta gtatcttagg
    12301 agcaaaagct tttggagaaa tagaaaatag taaaaaagta gtgatagctg ctgtaaacgg
    12361 atttgcttta ggtggaggat gtgaacttgc aatggcatgt gatataagaa ttgcatctgc
    12421 taaagctaaa tttggtcagc cagaagtaac tcttggaata actccaggat atggaggaac
    12481 tcaaagactt acaagattgg ttggaatggc aaaagcaaaa gaattaatct ttacaggtca
    12541 agttataaaa gctgatgaag ctgaaaaaat agggctagta aatagagtcg ttgagccaga
    12601 cattttaata gaagaagttg agaaattagc taagataata gctaaaaatg ctcagcttgc
    12661 agttagatac tctaaagaag caatacaact tggtgctcaa actgatataa atactggaat
    12721 agatatagaa tctaatttat ttggtctttg tttttcaact aaagaccaaa aagaaggaat
    12781 gtcagctttc gttgaaaaga gagaagctaa ctttataaaa gggtaaaaat ctaattaaaa
    12841 atatatttgc tgttaactag attaaatagt attttaatct agttaacaga atataaatac
    12901 taaaataaca ggaggttata actatgaaat tagctgtaat aggtagtgga actatgggaa
    12961 gtggtattgt acaaactttt gcaagttgtg gacatgatgt atgtttaaag agtagaactc
    13021 aaggtgctat agataaatgt ttagctttat tagataaaaa tttaactaag ttagttacta
    13081 agggaaaaat ggatgaagct acaaaagcag aaatattaag tcatgttagt tcaactacta
    13141 attatgaaga tttaaaagat atggatttaa taatagaagc atctgtagaa gacatgaata
    13201 taaagaaaga tgttttcaag ttactagatg aattatgtaa agaagatact atcttggcaa
    13261 caaatacttc atcattatct ataacagaaa tagcttcttc tactaagaga ccagataaag
    13321 ttataggaat gcatttcttt aatccagttc ctatgatgaa attagttgaa gttataagtg
    13381 gtcagttaac atcaaaagtt acttttgata cagtatttga attatctaag agtatcaata
    13441 aagtaccagt agatgtatct gaatctcctg gatttgtagt aaatagaata cttataccta
    13501 tgataaatga agctgttggt atatatgcag atggtgttgc aagtaaagaa gaaatagatg
    13561 aagctatgaa attaggagca aaccatccaa tgggaccact agcattaggt gatttaatcg
    13621 gattagatgt tgttttagct ataatgaacg ttttatatac tgaatttgga gatactaaat
    13681 atagacctca tccactttta gctaaaatgg ttagagctaa tcaattagga agaaaaacta
    13741 agataggatt ctatgattat aataaataag aatttgggaa ttaaaagttt aaataaaatt
    13801 gtttaaaaaa caatttcgga tatatgaaaa atctaattta atgggggtaa tgaatatgag
    13861 agaagtagta attgccagtg cagctagaac agcagtagga agttttggag gagcatttaa
    13921 atcagtttca gcggtagagt taggggtaac agcagctaaa gaagctataa aaagagctaa
    13981 cataactcca gatatgatag atgaatctct tttaggggga gtacttacag caggtcttgg
    14041 acaaaatata gcaagacaaa tagcattagg agcaggaata ccagtagaaa aaccagctat
    14101 gactataaat atagtttgtg gttctggatt aagatctgtt tcaatggcat ctcaacttat
    14161 agcattaggt gatgctgata taatgttagt tggtggagct gaaaatatga gtatgtctcc
    14221 ttatttagta ccaagtgcga gatatggtgc aagaatgggt gatgctgctt ttgttgattc
    14281 aatgataaaa gatggattat cagacatatt taataactat cacatgggta ttactgctga
    14341 aaacatagca gagcaatgga atataactag agaagaacaa gatgaattag ctcttgcaag
    14401 tcaaaataaa gctgaaaaag ctcaagctga aggaaaattt gatgaagaaa tagttcctgt
    14461 tgttataaaa ggaagaaaag gtgacactgt agtagataaa gatgaatata ttaagcctgg
    14521 cactacaatg gaaaaacttg ctaagttaag acctgcattt aaaaaagatg gaacagttac
    14581 tgctggtaat gcatcaggaa taaatgatgg tgctgctatg ctagtagtaa tggctaaaga
    14641 aaaagctgaa gaactaggaa tagagcctct tgcaactata gtttcttatg gaacagctgg
    14701 tgttgaccct aaaataatgg gatatggacc agttccagca actaaaaaag ctttagaagc
    14761 tgctaatatg actattgaag atatagattt agttgaagct aatgaggcat ttgctgccca
    14821 atctgtagct gtaataagag acttaaatat agatatgaat aaagttaatg ttaatggtgg
    14881 agcaatagct ataggacatc caataggatg ctcaggagca agaatactta ctacactttt
    14941 atatgaaatg aagagaagag atgctaaaac tggtcttgct acactttgta taggtggtgg
    15001 aatgggaact actttaatag ttaagagata gttttagatt ataatattta caaattaaat
    15061 atttaattat gaatttaacc tgtatgaata actatttgtt attcttacag gtttttatag
    15121 ttaaaaataa ttattatcta ttaaacaaaa atagtgtaaa atatataata tcatgatttt
    15181 gaagatataa aaagcaggaa gatataaaag aaagtaggta aaagtttgtt taaaaaagag
    15241 aatcaaagat ataatcttat atcgtataca aatgaagaag aaatgacttt aaaagaagtt
    15301 ttattggaca agctaaattt ctcagttagg tcattatcaa aaatgaagaa ggaaaagagt
    15361 gttttagtaa atggagtata taaaaaacca agtttaaagg tgtatagtgg tgatttaata
    15421 gaagtaaaaa tagacgagga aaaagcaaat tttgaacctc aagatttaaa tttacagata
    15481 atttatgatg attttgatat aattatggtg aataaacctc catttatggt agttcaccca
    15541 acaaaaagcc actatgataa aacaatagca aatggtatta gttactatat agataatcaa
    15601 aaagaaaacg taaaaataag atttgtaaat aggttagata tgaatacttc agggttagtt
    15661 atagtagcaa aaaatgctta tgcacatcat actttatcta cagcaatgag tgaaaataaa
    15721 gttgaaaaaa agtatataac agtagttgat ggaattataa aagaaaatga gggaactata
    15781 gatgagccaa tttatagacc tacagaagat tcaataaaga ggataataga tgaaagagga
    15841 cagtcatctg ttactcacta taaagtaata gaaagactag aaaatgcaac tgtattagaa
    15901 gtaagcttag aaactggtag aacacatcaa ataagagtac atatggctca tataggtcat
    15961 ggaataatag gtgatgagtt gtatggttat gtagatgaag agctaataaa taggcaagca
    16021 cttcatgcat acaagctaga gtttgaacaa ccaagaacaa aagaaaaatt gaaatttaaa
    16081 gcagatatac cagaggatat gaaagagtta atatcgaaat taagataatg gggtgatata
    16141 tatgataata cataaattta taatacatgt tttggacaaa aatagtgatg ttccaatatt
    16201 aaatgacttt gaaggcaaag ttaatcaaga agtagatggt tttttccaga aagctataaa
    16261 aagaattgca aaagatgagg atttaagaaa aggtgttttt aaagattata atgacaattt
    16321 aattaaaaac tgttgtgaac aaataatata tgatgaaagt acttttttga aaaattctaa
    16381 agaaattgca tcttacttgt ttgacgttat gaaaattaat gctgcactag aatcttgtga
    16441 tttggcaatt tgcttataca ctattaaaga tgaaaagagt gtagcaatac tgaaattaga
    16501 ttataaaaag ttgtacactc attcaattga atttgtggat gataaattta atattcaaat
    16561 ggtttcaaat gaaattggta tcccagagac tttaagacaa aagcaaggag ctttgattag
    16621 tttaagtggt ataaatgatg agtttcatct aaggctttta gataaggatg ctgaaaaaga
    16681 agggtcagaa tctaagtttg taacagaatt tttaaatgct aaaaaaatag atgatgataa
    16741 gtacaagacc aaggtattta aaaatacagc tgaaaattgg ataactaatg ctcttagtaa
    16801 tgatataaag caagctgaag atgtaagaag tatattaaat tatacactga aggaaaaaca
    16861 tgaagttgat ataaatgact ttgttgataa ttcaattaaa gatgatgagt taaaggatag
    16921 ctttaaagag catatggaag aaaaaggtct tgatgaaagt tttagtatag ataaaaaatg
    16981 ggttgagaaa aagcttaaaa aaagaagtat aaaaacggat aatggttttg acataaaagg
    17041 gaatctaact gattttgaag acccaatgaa gtatactgta aagcaaaatc aagatggaac
    17101 tatagatata ataataaaga atgttacatt ttatgaagaa aagtaattta tatacttagt
    17161 atataaatta aatataagag cgtgtatttt atatattgta aataaatgta atctagtctg
    17221 tgcgaactct aatataattt attatgtaga agtttacaca gactatttat atttgtagac
    17281 aaaacgatac tatttgtttt cttcgatata tttacatata tcaccaatac atttaaagtt
    17341 ctcagcttcc tcgtctggta tttctattcc aaattcatct tcaagggcca ttataatttc
    17401 aacagcatct aatgaatcag cttctaagtc gtccattaaa gatgcttcca tagttatctc
    17461 atctaaattg tctaccccta attgttctgc tattatttct tttattttct caaacatttt
    17521 aagcctccta aagcagtttt aattaatttt aatgttagtt taatataata tcaagaattt
    17581 ttcaatatgg aatctataaa aaataataaa attttataaa tgtagtaata aagtattatt
    17641 tgatgaataa actttattga taacaaatat aacttaggag gaaatgctat gaaatatgta
    17701 ggactattac taacttcagt aggaatgttt ttacttatag cagtaaattt ttattataat
    17761 tcaataactt tagatatgca acgtatagag gattatgtaa tggagaccaa tttaatcctt
    17821 gaagatgtag ctgaaaagga gagttatgta tctaatgaaa aagaagacta catatcaagg
    17881 ttaatgcatg taaagaaagg aatagaaaat tcaaaaacat ccttcttgat agaaagatat
    17941 aaggagtaca aaataaaatc aatagaaagt ttaatctaca ctatatcaga ggagaaaaaa
    18001 gattatctgg atgaagttga tagatacaat aaacttggtg aaaaagaaat aaataaacta
    18061 atcaataaaa actttttaga agtaacttat ctgtctatta ctacatatat ttaattaaag
    18121 agacaaaact tagtctctta atgattcaag gaggattagt tatgacaaaa ttagaagaca
    18181 atatggaacg tttctttgga ggatgcgaag gattttttgg taataaaatg ttgataataa
    18241 tagttatagt attcttacta ttatgcacag acattttaga agacttatta tgcgatgaca
    18301 atatatggat atgggttata ttaatagtat tgttattatt taacttcgat gatggttgtt
    18361 gttgctaaaa aatattgatt attaaatagg taaaaccagt aacttataca agttatggtt
    18421 ttaccttttt tagttcaaat taaaaaattt aaaaaaatag taaaaataca ttagcaaaat
    18481 ggaaaccaaa tgcatagtat atgttaggag cgaaaagctc aacaaaatat caacaggagg
    18541 taacgtacaa tgttagatag attctttgga gaagaaggat gttttggtgg aggaggatgg
    18601 tggataatag tactattctt cctattctta gcttttggag atagctgggc agaatttgac
    18661 ataatggcat ggataccatt cctaatagtt ttattaatac tttgctgctg tggtggatgc
    18721 ttagaataag aacacagtta ttaaaaaaag gagggctttg ttatgttaga tagatttttt
    18781 ggagaagaag gatgctttgg tggaggagga tggtggataa tagtactatt cttcctattc
    18841 ttagcttttg aagattgctg gagagacatt gacataatgt catggatacc tttcttaata
    18901 ttattgctaa taacttgttc atgtggtgga ttctttgatg gtgaagtagg ttgcggctgc
    18961 taatacttaa atcattagaa taaaaaaatg ctggtatttt ataccagtat ttttttattt
    19021 tattttttaa tatgatttaa attaagaatg aatattatta aatttgttga atatagggta
    19081 taatataaaa tataatgtat ttaaatgtac ttgcttttat ggtaaaataa aaacttatat
    19141 aaatgtagtt aaaataggaa ccttcaaaaa tttggttcag ttttagataa atttagtaaa
    19201 ataaggagga tgaagatgaa aggcaatata acgataaaag atgttgctaa acaagcagga
    19261 gtgtcaatat ctactgtatc tagagttata aatgattcaa aacctgtaac tgatgaagtc
    19321 aaacaaaaag ttttagaggt tataaaagag actggatata taccaaatcc acttgctaga
    19381 agcttagtaa caaagaagag tcaattaata ggggtaatag ttccagaagt ttcagattct
    19441 tttgttaatg aggtgttaaa tgggatagaa gaagttgcta aaatgtatga ctatgatatt
    19501 cttttagcga atacatactc tgataaggaa caagaactta agagtataaa tctattgaga
    19561 gcaaaacaag tggaaggtat agttatgatt tcatggatag ttgaacaaga acatatcaac
    19621 tatatacaaa attgtggaat accagcgaca tatataagta aaactgctag aaattatgat
    19681 atatatacag taagtactag caacgaagaa gctacttttg atatgacaga gcatcttata
    19741 aagaaaggtc atgaaaagat agcttttata atgacgagta aagatgatac tgttttagaa
    19801 atggaaagac ttgctggtta tgagaaagca ctttcaaata acaatataga attagacaag
    19861 agtttgatta agtatggtgg aactgattat gagagtggat acaatagtat gaaagaacta
    19921 ttagatgatg gaataatacc tcatgcggct tttgtaacag gtgatgaggc tgccataggt
    19981 gctataaatg ctatatgtga tgctggatat aaggttccag aagacatatc tgttgcagga
    20041 tttaatgatg ttaagatagc tagaatgtat agacctaaac ttactacagt atatcaacct
    20101 ctatacgata tgggagcagt agcaataaga atggttataa aattaataaa taaggaatta
    20161 attgaaaata agaaaataga attaccttat agaattgttg atagagaaag tgttacagaa
    20221 agaaaaaaat aaaactatta aaatcaatca ggtagtgcct aaggcactac ctgatttttt
    20281 atgtcaataa tatcaaattt aataatagat taactaaaaa taattatcta attacttaaa
    20341 tagtttattt ttatctactc atgagggtta gactttttag ttttcttaaa taaaccttta
    20401 gatttttttt gttgaccacc tggtgtattt tttggtgcag gtgaatttga tttaccttta
    20461 gtaggtttag ccttcttagt tttagctggt ttagtatctc tttgattttc tgcatctgag
    20521 attttttcca ttatattatc tagttttttg cgttgatttt catctaaaaa tgctttgaat
    20581 tttgatacta cttcatctct tccatctaag ttcttaccta tttttataag ttcctccata
    20641 agttcatttt ctgattttcc tttgtaacta tcagccatct tttctatttt atccttgtca
    20701 atattttttt gtttagccat tttttctaat gactctgtat ctaatttttt cataaaaaaa
    20761 cccctttcta taaatatata ttgaattata ttcatactat ttatatatag aaataatatt
    20821 gaagtggtga attgtgagtg atatataata taaataaaaa ttcttataga taatatatat
    20881 gcactgagct ttaaaaatgg aacattaaaa tatgaaaaac atactttaat atagggcaat
    20941 tatataatat aagggggaat tgtcttgaat aacttactat ttttgattgg agtgattgca
    21001 ttgagtaatg gtaatatctc tatatttgat gataaaaata aatcaaaagg aaataataga
    21061 gttagaaaaa acaaaaaaaa tgcaaatgat aaactttcac agtctacaca tggcaattta
    21121 aggtttgatg caaatgatat aaaaagaggt ttaaggctca tggatttaag tgaagaagat
    21181 ttagaaatgg gaatggaaat aataacaaga actaaaaaat acatgtcaag agatgaaaga
    21241 aaaatactaa taaaaataga aagtgtttta gatttagtta gaggaataaa aaaattaaat
    21301 aatatagata tggtagatac agaagatgaa acggattttt tcagaaaaat ggatagtgat
    21361 gataaaaaga atatgatgat aaaggaaatt atagatgtat tcccagaaaa aagaaaaaat
    21421 tcagttgaaa aagctatagg aatgaaaaag aagatagatt tatttgcaga attattttta
    21481 ccagatgact ttggtgaagg tggatttagt ttgagctctt tggcaaatat aaacaatctt
    21541 ggtagtatga ataatctcaa attgttagga agtcttttaa gaggtgatga tagtaataat
    21601 gatgatgaag acaataatga tgagttaaat tatgaagacg aagaattaga ttgtgaagac
    21661 aaagaattgg atgatgatga gttaacagat gaaggtgaaa tggaggataa tgaagatgaa
    21721 ttagtagaag ataagaacac aggctatgaa aatatagaaa atgatgaaga tatagtatac
    21781 gatgaggaac tatacaacat taatgagcaa gaatccgaat acaattataa tgaaaaaaat
    21841 actaaaacta gagctaagaa aaaacgataa ttaactaaat acataaagaa aaaaggaaga
    21901 aaaagtaatg atagaataag attatctcaa ttactatata aaaccacttt aaaaaattca
    21961 aaatttatta tataataata atatgaaatc ctgaaacata cgataaggct tttttaaatt
    22021 caacctacaa atgaaatacg ggagaccgtg tctggtaaat tataacatgc ctagttagtt
    22081 tggacgttaa aggttactgg caagtcaccc acctgggagt gcctagggtg tgaatttact
    22141 tagccaccgg tgtttcggga caaaaaaagt gctactattt caaatagtag tacttttttt
    22201 attaatatgc atttttctac taaatacgcc taattattga taaattcaaa aagcaagtaa
    22261 caattaagat tataaaagca tagtattaat taggcttcaa aaagccaaag acttaataag
    22321 aatcgcttac agggagggca atgaaaaatg caagattata aaaaaaataa aagaagaatg
    22381 atgaatcagc caatgtctac aatgaatgaa gaagaagtgt atacagatga aataaattca
    22441 gaagacatga gagggtttaa aaaatcacac catcataatg gatgtaatac tgataataag
    22501 tgtgagtgcc atgatgagtg taatccatgt aacccatgta atccatgtaa acctaaccca
    22561 tgcaatccat gcaaacctaa tccatgtgat gacaaatgtg aatgccatga caagtgtaaa
    22621 tgtgattgtg aaccatgtga aatggattca gatgaatgtt ttgaaaacaa atgtggacca
    22681 gaatgttgta atccgatatc tccaagaaac ttctctgtat caaatgcagt gccatttgca
    22741 atagaggcta atagaatatt tgatactatg caattccaaa catttacaga tgcaacaggt
    22801 ccaaatggag aaccattaac ttttgaaaca gaagtagtag aagtatttgg ttcaattcca
    22861 agtgcaggtc aagcaagtgt aactatagaa aaaatatgct taagtaatga tggaatagtt
    22921 atagacacag gaatgacaac tttagaagat ttcgatttag acccattagg agatatagta
    22981 ggaagaaact gtgaaacaac ttttgaattt gcagtttgtg gagaaagaaa cgctgagtgc
    23041 tgtagacaag gaaaaggcaa atcagtagct tataaacaaa gaggattaac tgtagcagtt
    23101 cgtaatttag tactagagct aagaggtaga tgtggatgta cagagttcgt tgcattagct
    23161 ttcccagcag ttagagcagg aggtggatgt aagagaagag ttgattatgt agaatttact
    23221 tttaacacac tttcagcacc aatatgcttg ccagctgacg gaagagctgt tactttaaga
    23281 caagaatatc aaactaactt aactgtagat tgtataggaa aatctatatt aaaattagaa
    23341 tgcaacgaat gttgtgaacc tttctatgaa ttaattatac caaatgatat agatttagta
    23401 ctttgcttac aagaaacagt tagcacatta ataagtgaac aaatagtagt tttagcatca
    23461 ccaaatccaa tccaaccaag acttgttgat actttctcta aagtatgtga tttttcgcaa
    23521 tgtggaccta atcatggaag tggaaagcca agttgccaca gatagatagg taagatatat
    23581 agacagttta aattataaat aaaaatcatc taaggaaggc taccaaagtg tataatttac
    23641 attggtagcc ttcacgtttt tataactagc gtatagattt tagtgattta aaaagcacta
    23701 tagtatgtaa tagacaagta aagattgata ctatcgtcat tgtgtatatg tatgcataaa
    23761 tattaagtcc tggaattgga agaaatacat ataaagctat tagttgaatt aacatgccag
    23821 taatagtatt tatacttgaa gctaattctt ttcttattga atgtaaaatg ccagatagag
    23881 tttgatttag ggacataaat agtggaacta aaaacatagc ttttagatat tctcctgcaa
    23941 gtgtattttt aaatactaat atacataaat cttttccaaa gaaataaaaa aatattgatg
    24001 atagtatgcc tacaaataaa gtaagaagta tggagtattt gattttttta attacactct
    24061 tacgctttct tagagccatt tcctgtgaaa tagttggtat taaatttaca ataagcgctg
    24121 aaccaacagt aaaaggtaaa aatataaagg gcattaccat cccactgaca ataccataca
    24181 tacttaaaga ttgttgataa gtcattcctg ataatgctaa tcttgatggt atcatcatag
    24241 aacttataga ttgcaaaagt atgtttgaca ttctattaca agttatggga atagacattt
    24301 ttaatgtttc catagaagaa ttataaaaat cttttatgct tataatatac ctattgtcaa
    24361 atgatgaatc tcgccataag caaataagca taaatataat attgctcaac tcaccaatag
    24421 atatacctaa tagagctata taacaattca tagatttatt atttatgtac ataacaagta
    24481 aaaaaacaaa taatatttta ccaatttgtt caatgatttg tccaatagca gggattttta
    24541 cattctttat gccatagtaa tatcctctta gtacgtttga aagagttatt ataactatag
    24601 caggacaaac agctagtata aatagattta atttagcatc ttttaaaaat ttaagtgata
    24661 aaaaagaact attgaaagat gcaactatag atataattag tgctacgaaa aatgatatat
    24721 ataaagttga tataaataga gcattagtat tgtgtctatc gtttaaagct tttctctttg
    24781 caactagaca actaagtgca gtaggtatac ctgttgttgt aacagctaag caaatcatca
    24841 aaaagttaaa aatcatatga tagataccta aacctgtttc acctagtgct cttgataaga
    24901 atattttata taaaaaacca aatattctta caataaaatt ggatataaat aaaataactg
    24961 ttgatagtac taaataaggt actttcaaaa aaatcacctc cttatctttt aaatatattc
    25021 acaaggaggt gtaaaaattt ataattttat aaattctggt ttcaatttat taaatttaga
    25081 tacatattta aatcccatat tttttagtaa tgaatatatg tagtcaaact cacatgcaac
    25141 ttgacttata tgatgtgaat ctgaacctgt ggttatgatt tcccctccca agtctttgta
    25201 catttgaagt atatacttag atggggttag gtctggaaga ttatatctat aacaagaggt
    25261 atttatctcg atacctttgc catcataaat agcttgttta agagtttctt caattatatc
    25321 agaaaatagt ctatcatcta aaatagtgtc ataaggtgca taccttttaa tcaaatctaa
    25381 atgacctaag acagagtaat ctttataatt cttaacaata ttatataaat aaagatagta
    25441 attttcataa acttcatatt gagttttatc tttaaaataa ttaccaaggt ataagtccat
    25501 tgtatctata gcatggatag aacatattat aaagtccaat ggatattgat gtgcttcctt
    25561 tttgtatgta tccattaatt gagtttgaac tccaaattca attccttttt ttattgatat
    25621 tttatctttg tatttgcttt gaagagtctc taaactctta aaataatctt cgtagacaat
    25681 tgaaaaagaa tcatcagcat atacatcata gtcaacatgg tctgtgaaac aaatttcctc
    25741 caaaccgagt tcaatagatt tttttaccat ttcttccatt gtagattttc cgtctgtaga
    25801 gaaacttgag tgcatgtgat agtcgtaaaa cataaatatt tccccttttt ataaaaattt
    25861 tggtattata tatacatact aaataatatc aaatatatat aaaagcatca ataaaatata
    25921 aatacaatga gcggtgataa atttggaaaa atacagaata gatttaaatg agaaaaaatc
    25981 aactaaatat atacagatat ttaatcatat aaaagaattg ataataaata aagagttagt
    26041 agaacatgaa aaattgcctc caataagaaa actatcaaat tttttacaag taaataacac
    26101 tactatagtt aaggtatacg agcttttaga aaatgaagga tatgtatata aaattatagg
    26161 gagtggtact tttgtatcaa gttcaaattc ttataaaagc ggtatagata acaggaaaaa
    26221 taagattatt gtgcaaagag aagatttaat tcattttgac aatgggaatc catctaatga
    26281 tatgtttcct atcgattcat ttaaaaatgc tgtaaatatg gcattatcta aagatggtag
    26341 ttcaatattt gaatatgatg aaggtcttgg ctctgaagaa ttaagagaaa aaatggtaga
    26401 gtatctaagt gatggaaata taaaaactac taaggaaaat attcaaataa tatcaggtgc
    26461 tcaacaaggt atagatatag tttgtaaagg acttataagt tattcagatg ttgtttttat
    26521 ggaagaacct tcttacaatg gagcaattga agtgtttaaa agtagaggtg caaaaataat
    26581 atctatacct atgttagatg atggaattga tataggtatt ttaaaattaa agttggaaaa
    26641 aataaaacca agacttatat acattatgcc taattttcaa aatccaactg gaatatctta
    26701 ctcaacatat aaaaaaaaga aattgataga attggcagaa gaacatgatt tttatataat
    26761 agaagatgat tttataagtg attttgtttt tgaatcaaag gacaatagaa caattagaag
    26821 ttatgatgat aaaaatagag tagtatatat aaaaagcttt tcaaaaatac ttatgccagg
    26881 tcttaggata ggaattgtag aaatgccaaa tgagttgtta aaaaaagttt tatgggctaa
    26941 atattcatca gatatatcaa caccaggttt aattcaaaaa tcaatgtatt actatatgaa
    27001 taattttgat tggaagaatt atttgagtca tatagagaag atatatacta ataagtataa
    27061 attgtcaaaa aacttgatta atgacaaatt gagtggtaaa ttaaaagtta gaaaatcttg
    27121 tggaggaata aacttttttt tagaattacc aagaggatat acatctcaag catttacaaa
    27181 ttttatgtta aacaaaggag tgtcaatgct tccaggaact tatttttttg acaatctagt
    27241 ggatgataga ttctttagga taaatatagc tcgcccaagc atggaagaac tagaaaaggg
    27301 aatatctgta ataagtgata atatagatga gtttttttgt gaatataaaa acaagttaga
    27361 tataaaaagc aataaattat tttattaaat aaatagaggt tttttaggag gaaaaaatgt
    27421 ttgacaagaa gaaattagat aggattaatg aattagcaaa gaaaaataaa gagggaatcc
    27481 tttctgctga tgaaatcaaa gaaagagaaa ttttaaggaa agaatattta gaaaacttta
    27541 gagctcactt tagatcaaga ctagatagtg tcaaagtagt ttcaccagaa gaatatgagc
    27601 aatacatgaa aaacaataaa aattagaact tagtaggagg aaaatatgaa cttaaagtac
    27661 gaatttaaga atgcttggga ttttgaaaga aaagaaaata ctattgaaca aattatgcaa
    27721 tattctcgca attatatgga gtttttaagt aagtcaaaga ctgaaagact ttcagtaaaa
    27781 gaaattataa aattggctaa agaaaataac tatatttcaa tagatgaagc tatggaaaaa
    27841 ggaagcataa gttgtggtga taagatttat gttataaata aagagaaggc agtagcactt
    27901 tttgtaatag gtaaaaatta tatagaaaaa ggtatgaaga ttataggaag ccatatagat
    27961 tcaccaagat tagatttaaa accaaatcca ctgtatcaag agagtaatct aggttttttt
    28021 aagactcatt attatggtgg aataaaaaaa taccagtgga cagctcttcc acttgcttta
    28081 catggtatag ttatattaaa tgatggaaca aaagtggaca tatctattgg ggaagaagat
    28141 agtgacccag tattttgtgt aactgattta ttgattcatt tggctggaga ccaaatgcaa
    28201 aagaaactat cagaaggaat tagtggagaa gctttaaata tattaattgg gaatattcct
    28261 ttagaagatg aagaaaaaga acctataaca gcaaatatat taaagatttt aaatgagaaa
    28321 tacaatatag ttgaagaaga cttattaagt gctgaaattg aagtagttcc agctggtaag
    28381 gctagagatt tagggtttga taggtctatg gtgttgggat atgggcatga tgatagggta
    28441 tgttcatatg ctgctgtaaa agctatacta gaaactgaac aaccagaatt tacttcagtt
    28501 gcattatgtg tagataaaga agaaattggt tctaaaggga atacaggtat gcactcaaag
    28561 ttttttgaaa atactgtggc tgaactgatt gctttagaag gagattattg tgatattaaa
    28621 gtaagaagag ctttagcaaa tagtaaggta ttatcagctg atgtttctgc tggatatgac
    28681 ccaaattttg gagaagcata tgaaaagaga aattcagcat atatgggaaa tggagtagtt
    28741 ttaactaagt atacaggttc aagaggaaag agtggatgta atgatgctaa tgctgaattt
    28801 atgtcagaag taaagagaat ttttaataaa ggaaatgtag tatggcaaac tgctgaactt
    28861 ggaaaagtag accaaggagg aggcggaact atagcccata tattggcaaa tcaaggtgca
    28921 gaggttatag actgtggtgt tggagtttta aatatgcatg caccacatga gattgtctct
    28981 aaagtagaca tatatgaaat gtataaggga tataaggcat tttttaatat aaatttataa
    29041 gtaaaaatat aaatgtaata agtgaataaa ggctgttgta taaacaattt aatctgttgt
    29101 acaacagtct tatttttatg taaaaatagc aatttgtcac actagagaaa ataaaagaaa
    29161 atactgtcaa attaaaaatt atttaaaaaa aaattgacaa cgatttcatc ttgttataat
    29221 atgtaataaa gaacttgtta tataatgtta taaaaatgtt acatagggag gtgagcaaaa
    29281 tggatgaaaa gagacaaaaa aaagaaatgg atttagaagt aaattccttt gttcctttat
    29341 atcaacaact atatgacaac ataaaaaaac aaatagcatc tggcatatat aaaccaggag
    29401 ataaacttcc atctgaagga gacttatgta aagaatttaa tataagtcgt ataactgtga
    29461 gaaatgcact taatgaactt gtaaaagaag atatattatg taaaaaacgt ggtaaaggga
    29521 cttatgtaac aataccagag agaatagaag caacatgtgc aggaaatagt tttactaatt
    29581 cttgtcatcg tattaatgca aaaccaagta ccaaaataat ctctgtttta ataaaaaaag
    29641 cagataagca agtagctgaa gctttaagta ttgaactaga agagaaggta atatgtatta
    29701 aaagacttag attaatagat gaagtacctg ttatttttga agttgactat tttagaatag
    29761 attacatgtt tttattaaaa gaagaattag aagataaatc tttaatggag gttatatcta
    29821 ataatataag cacattacct aaacgagtag aaaatatatt tgaagtaaaa cattcaaaca
    29881 aagaatattc tgactatctt aaatgtgcaa gtaatatgcc actattgaaa gtaagacaat
    29941 cggtgtatac agaaaataat gatgtcttat attacaatga gcaatttata agaagtgata
    30001 agtacaaata tgcagtatca gcagaaatat aatctatatt tagtaggagg atgaagtatg
    30061 aaaaagataa tattagcatc tcatggagat ttttcaaaag ggcttttaaa tgcagtaaaa
    30121 atgatagtag gagatttagc agattgtgtt agttcgtatg gtttgtaccc tggacaaagt
    30181 gcttcagatt ttgctttaga attagaaaaa acaatatcag aagatataga atgtgaatat
    30241 ataattttat ctgacttata tggagcaagt gtatgtacag caatgttaag attaactaat
    30301 ctacataatg ttaagttatt ttctggaatg aatttgaata tggtattaga acttcttaca
    30361 aggttttctg atgaattaac agttgaagat atagaacaat tagtggaaga atctagaaga
    30421 ggaatacaaa gcgtgacatt acaaattaaa gaagaagaag aggtatttta gtttatgaaa
    30481 aaaaagggaa tattatcagt atttaaaagt aaaaaaccaa ttatagcaat gatacactta
    30541 aaaggagata ctccagaaga tatctttgaa agagctaaaa aagagattac aatctttgaa
    30601 gaaaatggtg tagatggaat aatgttagaa aattactatg gtaattatta tgatttagaa
    30661 agaatacttg aatatgtatc aaaagctaat ttgtcaatac catatggagt aaattgttta
    30721 aatgttgata ctatgggatt tgaactggca acaaaatata atgctagtta tatacaggtt
    30781 gattctgtag taggtcatgt taaacctaga gacgaagcta ctttagaaga attttttaaa
    30841 ttacaacgtt caaaatgccc agcgtattta attggaggcg tacgttttaa atatcagcca
    30901 gtattatcag aaaatgacgt tgaagaagat ttaaaaatag gaatgacaag atgtgatgct
    30961 atagcagtta cagaaaatgc tacaggtcaa gaaacatcaa tggaaaaaat agaactgttt
    31021 cgtaaaaatt taggagactt tccattggtt atagctgctg gagttacatt agaaaatgca
    31081 aaaaaacagc ttgaattagg agatatggca ataataggaa gttactttaa agataattac
    31141 aaagattttg ggaatgttag tgtagaacac gtaaaaacat tcatggatga aatcaaaaaa
    31201 attagggagg aattataatg attaaattag taagagtaga ccacagactt atacatggac
    31261 aagtagcatt tacatggaca aagtttttaa gcacagactg tatattgata gcaagtgatg
    31321 aattattaaa agatgaatta agaatggcag ggcttaaaat ggctaaacca tctaatgtta
    31381 agttagtaat gaaaagcata gcagattcta taaaagcact taactcaggt gttactgata
    31441 aatataactt attgatactt tgtgaatctg tagaagatgt ttatagactt gctaaagagg
    31501 taaaagctat taaatctata aaccttggtg gaacaaaatc agatgataat cgtgaaaata
    31561 tatctaaagc agtgcatgta tcaaaagatg atataaagat gattaaagag ttagattcag
    31621 aaggcgtaaa tgtatttgta caattagttc ctgatgatga tgctacaaat gtaatgaaat
    31681 taatataatt ttagggggat gaaaatggaa tttacacaag ttattttgat aactctaatt
    31741 gcattctttg catatatgca tagttttgta ggttctacaa tgcataatag acctatagtt
    31801 gttgcaccac ttgttggttt agcactaggt aatcttcata caggtatagt aataggttca
    31861 acattagaat tagtatttat gggtgcattt cctgttgggg caagtaatcc tcctgatttt
    31921 gtgtctggtt ctattattgc cacagcgttt gtaatattaa cgggtcaaga tgtatcagca
    31981 gcagttgtac ttgcagttcc tatagcaact ctagtattgc ttatagataa cttcttaatg
    32041 acagtagtac ttacatgggg agctcatatt gctgaccgtt atgcagaaga ggggaatatt
    32101 gaaggcgtag aacgtgtgca attattattt ggtataggaa ataaattaat attagcaata
    32161 atagtaggta taggattttc attaggtgtt ccagtaatag aaaaaatact ttcatttata
    32221 ccatcatatg taactcatgg aatggatgtt gcagcaggcg tgattccagc aataggtttt
    32281 gccatgttag ctaggatgat gcttaataag aaaacagtag catttttatt attaggattt
    32341 atattggttg cttacctaaa cataacagta acaggagttg cattgtttgg attagcaata
    32401 gctttaatat atgttaactt tgtaggagag aaggaggtaa tagtagatga caacgagttc
    32461 taagaagtta gatacaattt cacccgatag taaaataacg aggaaagact tttggaaatg
    32521 ctttagaagg tcattgacat tagattcatc ttggaactac gaacgtatgc aaaatatagc
    32581 atatgcatac atgatggcac caataatccg tagattgtat aaagatgata aggaaaagaa
    32641 atctaaggct ttaaaaagac atttagaatt tatgtcagtt actcctcata taagtactct
    32701 tttggtaggt atatctggag ctatggaaga agaaaatgca aagaataaag aatttgatgc
    32761 taatagtata aatgctgtta aatcaagttt aatgggtcca gtttctggga taggagattc
    32821 atttttctgg ggaacattaa agcttatagc agctggagtt ggtatagctt tagcttctca
    32881 gggtaacata atgggaccga ttttattcct tttaataata aatgttcctc attttattat
    32941 acgctatata tgtctagata aaggatttaa gtatggaact caattcttta aagatgtgag
    33001 tagttcaagt atagtttcaa aagttatgga agcagcttca atgttagggc ttatggtaat
    33061 tggaggtatg actgcatcta atgtaatgtt aaaacttagt gttaatgtag gtagtggaga
    33121 atgggcagag cctattcaaa cttacttaga ccaaataatg ccatgtatgc ttccagctat
    33181 gatattcggt attatgtatt ggttattagg aaaaaaggta aagacaacta cgatattaat
    33241 ttcagttatg attatttgta tagtattagc tgctattgga atagtataaa taaaaaaatc
    33301 ccaagttcat ttgaacgagg gattttttga ctaaattaaa taaaaaagaa agatggttca
    33361 taataattta taagcattta tgtgatagtt ataattagct tattaa
//
LOCUS       DAEPLF010000022        33269 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEPLF010000022 DAEPLF010000000
VERSION     DAEPLF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.022"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            236..376
                     /locus_tag="KR314_000933"
     CDS             236..376
                     /locus_tag="KR314_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327331.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(551..871)
                     /locus_tag="KR314_000934"
     CDS             complement(551..871)
                     /locus_tag="KR314_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBH0327332.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(991..1728)
                     /locus_tag="KR314_000935"
     CDS             complement(991..1728)
                     /locus_tag="KR314_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBH0327333.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1943..3766
                     /gene="acd"
                     /locus_tag="KR314_000936"
     CDS             1943..3766
                     /gene="acd"
                     /locus_tag="KR314_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBH0327334.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYIS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3981..8279
                     /locus_tag="KR314_000937"
     CDS             3981..8279
                     /locus_tag="KR314_000937"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBH0327335.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEILPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8483..8944
                     /locus_tag="KR314_000938"
     CDS             8483..8944
                     /locus_tag="KR314_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBH0327336.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8974..10128
                     /gene="nusA"
                     /locus_tag="KR314_000939"
     CDS             8974..10128
                     /gene="nusA"
                     /locus_tag="KR314_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBH0327337.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVKSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELGTSEDNFSEEE"
     gene            10135..10416
                     /locus_tag="KR314_000940"
     CDS             10135..10416
                     /locus_tag="KR314_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBH0327338.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10406..10717
                     /locus_tag="KR314_000941"
     CDS             10406..10717
                     /locus_tag="KR314_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBH0327339.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10739..12679
                     /gene="infB"
                     /locus_tag="KR314_000942"
     CDS             10739..12679
                     /gene="infB"
                     /locus_tag="KR314_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBH0327340.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12722..13102
                     /gene="rbfA"
                     /locus_tag="KR314_000943"
     CDS             12722..13102
                     /gene="rbfA"
                     /locus_tag="KR314_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBH0327341.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDNLIKRVNEKNAQQNEDENYDE"
     gene            13083..14066
                     /locus_tag="KR314_000944"
     CDS             13083..14066
                     /locus_tag="KR314_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBH0327342.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNVGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEGVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQTKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSDYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            14079..14984
                     /gene="truB"
                     /locus_tag="KR314_000945"
     CDS             14079..14984
                     /gene="truB"
                     /locus_tag="KR314_000945"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBH0327343.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEVVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            15115..16044
                     /locus_tag="KR314_000946"
     CDS             15115..16044
                     /locus_tag="KR314_000946"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBH0327344.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEYAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            16148..16405
                     /gene="rpsO"
                     /locus_tag="KR314_000947"
     CDS             16148..16405
                     /gene="rpsO"
                     /locus_tag="KR314_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBH0327345.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16645..17490
                     /locus_tag="KR314_000948"
     CDS             16645..17490
                     /locus_tag="KR314_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBH0327346.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            17615..19726
                     /gene="pnp"
                     /locus_tag="KR314_000949"
     CDS             17615..19726
                     /gene="pnp"
                     /locus_tag="KR314_000949"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBH0327347.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19992..20735
                     /locus_tag="KR314_000950"
     CDS             19992..20735
                     /locus_tag="KR314_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0327348.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            20893..22140
                     /locus_tag="KR314_000951"
     CDS             20893..22140
                     /locus_tag="KR314_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBH0327349.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAICLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            22175..22420
                     /locus_tag="KR314_000952"
     CDS             22175..22420
                     /locus_tag="KR314_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBH0327350.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22430..23653
                     /gene="dapG"
                     /locus_tag="KR314_000953"
     CDS             22430..23653
                     /gene="dapG"
                     /locus_tag="KR314_000953"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBH0327351.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEENIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23766..24500
                     /locus_tag="KR314_000954"
     CDS             23766..24500
                     /locus_tag="KR314_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBH0327352.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            24623..27034
                     /locus_tag="KR314_000955"
     CDS             24623..27034
                     /locus_tag="KR314_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBH0327353.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKSNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKSSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            27037..28095
                     /gene="mnmH"
                     /locus_tag="KR314_000956"
     CDS             27037..28095
                     /gene="mnmH"
                     /locus_tag="KR314_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBH0327354.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESVFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            28089..29423
                     /gene="rimO"
                     /locus_tag="KR314_000957"
     CDS             28089..29423
                     /gene="rimO"
                     /locus_tag="KR314_000957"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBH0327355.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFSELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            29410..29952
                     /gene="pgsA"
                     /locus_tag="KR314_000958"
     CDS             29410..29952
                     /gene="pgsA"
                     /locus_tag="KR314_000958"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBH0327356.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            30240..31286
                     /gene="recA"
                     /locus_tag="KR314_000959"
     CDS             30240..31286
                     /gene="recA"
                     /locus_tag="KR314_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBH0327357.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            31462..33003
                     /gene="rny"
                     /locus_tag="KR314_000960"
     CDS             31462..33003
                     /gene="rny"
                     /locus_tag="KR314_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBH0327358.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT        13479 a         3527 c         6351 g         9912 t
ORIGIN      
        1 atctatataa atatattcat tatatctcaa tttaatatat aaaaaagata ataatagtta
       61 gactttttat atgaatttta ataggtaaaa tatcctattg ttttgtagat atatcctaat
      121 tatgtcaaaa aataaaaaaa tttttattta ttagagtttt tggaaataat aataacagat
      181 attataatta attaacagtt tttgtaagta tttgacaata gagagggtgt aacaaatggg
      241 aataatagag tcagcatcaa aattggctga gatggttcat ttattggctg ttgaaaaggg
      301 gataactgat atagaagcat gggatgaagc tgtgaaagaa tacagcaaaa tttatgaaga
      361 aagaagaaat gaatagaatg ataaaaaatg aaaacttgat gataaaaaat aactttattt
      421 aaagaaattc aatttttata gaaatattaa aaataatata aataaaaaga tggtatttca
      481 aatgtaagta aaaaatcatt ttgggatacc atccttatta tttattttaa taatatataa
      541 gtaataaatc ttacttatcc atatttttta tgtaacctct tttttctagc ttttttatat
      601 gctccttacg tatttcatca agattaatac catattgttc ttctagctta aacatcatag
      661 taacacatgt ctgagctaca tccattagtt ccttagcagt tttctttata atatcaactt
      721 catctaaatc attattttct ccacttagat ttctgaattt acctatacat tcagctaatt
      781 caccttgttc ttcacccatt ttgataaatg tagattctag tgttgggtct aaattattaa
      841 gctctggtaa agatatagtt tttagtttca taaaagctcc tttcaagtta ttcgtattta
      901 tactaaaaca tcataacaaa aataatacta aaaaaaaact ttaaattcaa cataaaaact
      961 tacatatata ataaaaatcg tgaaataaat ctatatttgt cttaatacag cctcatataa
     1021 aacttctcca ataggtttat ttattacaag agaagctgac ttatcataga ctgtatttgc
     1081 tttattaata agagcaatat aatcaccttt gtaataatct ataaaacttg ctgctgggta
     1141 cacagctaat gatgtacctc ctataatcaa taaatcagca ttacttatag cagaaattgt
     1201 tttagtaatg acatcactgt caagagcttc ctcataaagt actacatctg gttttactat
     1261 agaaccacag ttgtcacaat aaggaatatt acctcccaga tttaacatag attctaagtc
     1321 aaaaaactta ccacatttag tacaataatt tctatgtaca gaaccatgaa gttctagcac
     1381 atttttactt ccagccattt gatgaagtcc atcaatattt tgagttatga cagctttaag
     1441 cttacccatt tcctcgagtt ttgctaaagc tatatgagca ttgttgggtt ttgcattagg
     1501 atatattaat ttatccttat aaaattcaaa aaattcttct ggatatctaa caaaaaaagt
     1561 atgtgaaact aattgttcag cagtaaattg tttattgagt ttttggctaa ataatccagt
     1621 agaacttcta aagtctggaa tattagattc agttgaaaca cctgctccac caaaaaatac
     1681 aatattattg tgattagcta ttaagtcttt taagctattt gcatccatag ttacacctcc
     1741 aaattattat atataaattg tactacatat taggtctttt atgttttatt aatttaaata
     1801 aatatgtagt ttaataagtt aaataaaggg aatataaata aaatgtatac agagtataaa
     1861 tagacaatat ttactaaaat aacaagttgt cgtataatta tattaaaatt gttttagtgt
     1921 ataagaaggg cgggggaagt acgtgaagaa aaaagcagct ttagcaacat tggctatgtt
     1981 acccttaggt gtggtaaatg cacatgctga tggagatata ggtatagtga ctataaatta
     2041 tttaaatgta agaaatgagc caactgccga aagtagcata gcctttgttg ctaaaaaaga
     2101 tgataaagtt ttgattaaag actcttctaa tggatggtat aaaataaaag ctgaatctgg
     2161 acaagaaggt tgggcttcat caaaatatat agcaaaatca aatagtgact ctttaagaac
     2221 gtctacaaat aaagaaaaac aagttatttc aaatagttta aatatgagaa atggagcagg
     2281 tacaagttac agagtaataa ctgtactaaa aaaaggtcaa aaagtagaag taatatcaga
     2341 gagtaatgga tggtctaaaa tcaaatatga tggaagacta ggatatgtat ctagctctta
     2401 tttaggagac gtttcaaatt caactaataa atcaaaaact aaacaagtga atacaacttc
     2461 actaaatgta aggagtggac caaatacaag ttatggtttg ttaggcaagt tgccaaaagg
     2521 aagtaaagta gaagtaatat cagaaagtaa tggatggtcc aaaataaagt ataatggaaa
     2581 agatgcctat atatctagta tgtacttatc ggatgtaagt caaagtaatt cagacaattc
     2641 tagtcaaagc aatgataaaa agaatactga taaggttgta aatacagctt ctttaaatgt
     2701 aagaagtgga ccaggttcta catatagtaa gttgggaaaa gtttataaag gaagtaaagt
     2761 aactgtacta tcagaaagta gtggatgggc taagattaat tttaacaata aagaagcatt
     2821 tgtagtaggc aattatttat ctacttcagc agatacttca aataataact caaatagtaa
     2881 ctctgataac agttctaata gtaatggcaa taattcttca tcatcaggtc aagtaaatgg
     2941 tatgtcaggt ataagtgggg ctaaaattga ttataaatct ttgagttata ctttagaatc
     3001 tcatataagt aaacaagttg agaaagcagc atcaggagga aatgtgatag ctccaagtaa
     3061 taggaaaagc actccaagtc ctgaatttag tactttttca gctcaaagaa caagctcatt
     3121 tgtaaatgca agttcaagtg atatagaata ttatttaaat cctaaaaatt ttacaaatac
     3181 tactaaaggt atgatgcagt ttttaaagat taacagttat agagatggta tttcagaatc
     3241 aagtcttaac tcgtatctta atggtttatc ttctagtgta tttaaaaacc agggagcagc
     3301 ctttataaat gctgccaaga agtataatat agatgttgta tatctagtat ctcatgctat
     3361 gtgggaaact gcttatggta agtctacact tgcccaaggt caaacattaa catcttataa
     3421 gggacaacct cttagtaagc cagttaaagt atacaatttt tttggtatag gagcaataga
     3481 taaaagtgct aatgtttcag gtgcagaagc agcgtattcc aatggctgga ctagtgtaga
     3541 agctacaata gatggctctg caaagtggat atcacaaaat tacgtaaata gttctaagta
     3601 caatcagaat actatctaca agatgaagtg gaattatgat tatacatggc atcagtatgc
     3661 aactgatgta aactgggcta atggaatttc tggaattatg gaaaatttaa ttggtcttta
     3721 tggaggagga agtagtttgg tttttgaagt tcctcaatat aagtaataca taaataaaaa
     3781 atatagacta tgggtagttt tcattttatt taaattatct atagtctttt tgtatataat
     3841 ttataatctg taaatttaat gttaacaaaa cttttatgtt ataatattaa ttctaagtaa
     3901 atataagtga gtaataggta gaaatagata aaaagtggta ataatatata taaagataat
     3961 tttgagaaag gaggatatat atggaaagta ttaaggaata tttggataag ttagaaatta
     4021 ataatagtgg tcttggaaag caacttaaag aagtttatat aaatagagta gtttatttta
     4081 aggaagataa gatagtttat ttttatctaa cttctaaaga tattgtttct catgaacttt
     4141 tagataaatt taaagaagaa ttgatgtata aactcgatta ttttaaagat atgaggatga
     4201 agataagatt tactggtctt gaaagaaaat ccaataaaga tgttataaaa aaatattgga
     4261 ataatatatt gtatatatta aagtatcttt gtccatctat tgcagggtgg tataaacaag
     4321 tagaatttct ttgtttagaa gaagaattaa aaataaaact tccaaaaggt atattttatg
     4381 agcgattaat gaaaaaaaat gtaacttatg tacttaaaac cgtgcttagc gaagagttgg
     4441 gattagattt aaatataaca atagaaaaag ctgttgatga aaaggtaaat aaagaaagac
     4501 tcataagaat caatgataga gaaatggaag aaaaaattag ggctttagaa attggaaaag
     4561 ttaataactg tgaagaaaat gaagaggaaa gctatgtcat taagtctgat gtagatgaaa
     4621 atttaatcta tggagataat gcaaatgcta tgattgaaaa tatagttgaa ttaaatgcat
     4681 cttctggaac agttgcagtt gttggagata tatttgatgt tgacactaaa gaacttaaaa
     4741 acggaaaaat acttatgata gcttcaatta cagattatac tagctcaata agctgtaaac
     4801 tttttttaac agatacaaat aaggatggag tacttgaaaa tgttaaaaag ggagcatact
     4861 taaaaatcaa gggtgacata gtttatgata catatcaaag agaaattagt atgatgatta
     4921 gtgggataag aaaagaaact agaatagaga gagaagataa atcagaggaa aaaagggtag
     4981 aactccatgc acatacacaa atgtcttcta tggatgctat ttgttctgtt aaaaagcttg
     5041 tagagcgtgc agccaaatgg ggtcattctg ctattgctat aacagaccat ggtgttgtac
     5101 aaggatttcc agatgctatg aatgcaggta agtcaaataa tataaaaata ctttatggag
     5161 tagaagggta cttggttgaa gatgattctc tcattataga ggatgcaaat gataaagaac
     5221 tttcgcagac atttgttgta tttgacatag agacaactgg tttttctaat acaaatgata
     5281 aaataactga gattggtgct gtaaaaatag aaaactttaa gattgtagat agatttagtg
     5341 agttaataaa tcctgaaaaa gatatttcct ataagataca ggaattgaca ggaataacta
     5401 atgaattaat taaagacaag ccaaccattg aagaaattct tcctaagttt atggaatttg
     5461 ttggagatag tgtattagta gcacataatg cagagtttga taccggattt atatcgcaaa
     5521 aatgtaggga acaaggcttg ttatataata acaaaaaagt agatacactt atgttagcaa
     5581 gagtaatgtt gccaaactta aaaagacata gattaaatgt cgttgcaaaa gaacttggta
     5641 ttcctctttt aaatcaccat agagcagtgg atgatgctga ggctacagca cttatattta
     5701 ataagttttt gcagatgctt acagaaaaag gtgcaaaaac tctaagtgat gtaaacaata
     5761 tacttggaaa gattgattac acaaagctta gcacaaatca tataaccttg atagcaaaaa
     5821 attctatagg tataaaaaat ttatataaaa taatttcaga tgcacatgta aatcattttt
     5881 ttagagcacc aagaatcctt agaagtgtat taaatgaata taaagaagga ttaataattg
     5941 gttcggcttg tgaagctggg gtggtttttc aagctgtaaa gaaaaacgtg tctgatgagg
     6001 aaatgaaaaa aataatagat ttatacgatt atattgaagt aatgcctata gataataata
     6061 gatttatgat tgataaagga gaagtaaagg atgaagaaga gttaagggag ttgaatagga
     6121 aattaattga tacagccaaa aaatttgaca aaattccagt agctactggg gatgttcatt
     6181 ttttagacaa acatgaagct gtcttaagaa aagttcttaa atattctcaa ggttttaaag
     6241 tagatgaaga agaaacttat ttacatttta gaacaacaga tgaaatgttg gaagagttta
     6301 gttatcttgg agaagaattg gcatatgaag ttgtagttga aaatagtaat ctgattgccg
     6361 atatggttga agatataaaa cctataccag atgatactta tcctccaatt atagagggct
     6421 cagatgtgga gttaagagaa atgtgttaca aaaaagccaa acgaatctat ggagacccaa
     6481 taccagaaat agttcaaaaa agactggata gagaattaaa ttcaataata agcaatgggt
     6541 atgcagttat gtatattata gctcaaaaac tggttgcaaa atctctttca gatggatatt
     6601 tagttggttc aagaggctca gttggctcat cttttgcagc tactatgagt gatataacag
     6661 aagttaatcc tcttccagca cattatattt gtgaaaacga agactgtaaa tattcattct
     6721 tttatgaaat aggtgaatgg ggctcaggtg ttgatttacc agacaaggat tgtccaaagt
     6781 gtggaagaaa acttgctaaa aacggtcatg atattccatt tgaggttttc ttaggttttg
     6841 aaggtgataa ggaaccagat atagacttaa atttctcagg aaattatcag ccaacaattc
     6901 ataaatacac agaagaacta tttggagagg ggtatgtata tagggctggg actataggta
     6961 ctgtagcaga aaaaacagct tttggatacg caagaaaata tgtggaagaa aataatataa
     7021 gtgtaccaaa tgcagaagta cttaggcttt caaatggatg tactggagta aaaagaacct
     7081 caggacaaca tcctggtgga gttatggtta taccagacta taaagatgta tacgatttta
     7141 cacctataca gtatccagca aatgatactt cttgtggtgt tataactact cactttgatt
     7201 atcattcaat aagtggtaga atacttaaac ttgatatact tggacacgac ggtcctacca
     7261 ttataaggat gcttgaagat atgactggaa taaatataac agaaattcct ttagatgata
     7321 aagagactat gtctctattt acatctacag aagcattagg tgttacacca gaagaaataa
     7381 actgtcctat aggttgcctt gctgtaccag agtttggaac taagtttgta cgacagatgt
     7441 tacttgacac caaacccact acatttgcag aacttgtgcg tatatctggt ctctctcatg
     7501 ggacagatgt ttgggtaaat aatgcacaag acttagttag agaagatata gttggattaa
     7561 aagatgttat atctacacga gatgacataa tgaactattt aatatttaaa ggattacctc
     7621 ccaaaatgag ttttacaata atggagagcg taagaaaagg taaaggtcta aaaccagaac
     7681 acattgaaga aatgaaaaaa aataatgttc cagaatggta tataggctca tgtaaaaaga
     7741 ttaagtacat gttcccaaaa gcacatgctg ttgcttatgt aatgacatca tttagaattg
     7801 catattgtaa agttcactat ccagaagcat tttatgcgac ttattttacg acaaaagttg
     7861 aggattttga tgcagattta attgttaaag gattagatgc tataaaatca aaaataaatg
     7921 aaattgagtc actgggaaat gatgcaacca caaaagaaaa aggtatgttg accgttttag
     7981 aagttgctct tgaaatgtat gctagaggaa taaaattatt accagttgat atatataaat
     8041 cagatgcaac tgagtttata gttgtaggag aaaaaacatt acttccacca atggcagcaa
     8101 tacaagggct tggaggaaat gcggctataa acatccaaaa tgaaagaaaa aatggtgaat
     8161 ttatatcaaa agaagatttg agaaagagaa caaaaatatc aaaaacggta atagaaactc
     8221 ttactaatca tggttcactt gagaatatga gtgagagaaa tcaattgtca ttgttttaag
     8281 ttagcccaaa aagttgcaaa taaaagaatt ttatgatatc atagtataga aaatagtata
     8341 ctttgcagaa actaagagtg agacagctgt tctcactctt ttattttaaa tatattatta
     8401 aaaagttggt ttatttttat taaatatatt ttatttaaga ttatataaac taatcattta
     8461 caattgaata ggaggaactt taatgaaaaa aaatttagaa gctacaattg aggagattgt
     8521 aactaaaata acggatgaac atggatttga aatggttgat gttgaatatg taaaagaagc
     8581 aggagaatac tacttaagag tatacataga taaagaagaa ggaatatctt taaatgaatg
     8641 tgaattagtt agtagagaat taagtccaat actagatgaa aaagacccta taaaagaaaa
     8701 ttacttttta gaggtatctt ctccaggact tgatagagca ttaaaaaaag acagagattt
     8761 tgttagatat caaggtagag atgtagattt aaaattatat aaaccattaa atggatgtaa
     8821 gcaatttgaa ggtgaacttg taggattaac agaagataat aatataaaaa tcattgcaaa
     8881 tggtaaggaa atggaattta atagaaaaga tgttgctatt gtcagattag caataaagtt
     8941 ttagtggtat attaaatagg gaggatataa aaaatgaatc atgaatttat ggaagcatta
     9001 gatgaattag tagttgacag aggtatagat aaagaaatac ttatagatac tatagaacaa
     9061 gctcttttaa ctgcatacaa gaaaaacttt ggttcagcac aaaatgttag agttgaattt
     9121 gatagagaca agggagatat aaaagtattt tctcaaagag ttgttgttga tgaatcagat
     9181 ttatatgata ctttcttaga aatagagtta gcggatgcta gagagataaa ccctaattat
     9241 gaattaggtg atattataga acatgaagtt acacctaaaa actttggaag aattgcagct
     9301 caaacagcaa agcaagtagt tgttcaaaga ataagggaag ctgagagaga aatagtgtat
     9361 aatgaattta tagaaaaaga aaatgaaata gttacaggag aggttgctag agcaaataaa
     9421 aatgttgttt atgtaaatct tggaagaata gaaggtataa tgactcaaag tgaacaaata
     9481 ccatttgaga attataaagc aggtcaaaaa ataaaattct atatacttga agttaaaaag
     9541 actaacaaag gacctcaaat agttgtatca agaagtcatc cgggtctagt aaagagattg
     9601 tttgaagaag aagttcctga aatatatgaa ggaatagttc aaataaaatc tgtagcaaga
     9661 gaagcagggt caagaactaa aatggcagta aaatctatag atgaaaaaat agacccaatt
     9721 ggagcttgtg ttggaccaaa aggacttaga gtaaaaaata tagtggatga acttggcgat
     9781 gagaaaatag atataataaa atatagtgaa gacccagcag agtttatatc agcagcactt
     9841 agcccatcaa aagttgttag agtagatata gatgaagatg aaaagtctgc cttagtaatc
     9901 gttccagatt atcagttatc gcttgctata ggtaaagaag gtcaaaatgc aagactagca
     9961 gcaaaattga ctaactggaa aatagatatt aagagcgaat ctcaagcaga acaagaaatg
    10021 ttaaatcttg aaaattccaa agaaatagaa gctgaggatg ttgaagctga atttatcgac
    10081 caagaagagt taggtacttc agaggataat tttagtgaag aagagtaagg agggatgatt
    10141 ttgaaaaaag taaaaaaagt gcctcaaaga aaatgcatag catgtcaaga tagagactct
    10201 aaaaaagagc ttataagaat agttaaaaat aaagaaggaa aaatattttt agacccaaca
    10261 ggaagagcca atggaagagg agcatatgta tgtaaaagct ctgaatgtct aaaaaaagct
    10321 ataaagagta aagcattaaa taaagctttt aaaatagatg ttccagatga agtctatgat
    10381 aatctactta tggagttaga agaatatgaa aaataataaa gaaaaagtac tttcgttctt
    10441 agggctcgca actcgtgcag ggaaaatagt gtctggtgat gattcaactt tgcttgacct
    10501 taaaaaagga aaagtaaatt tgattttaat cgcagaagat gcatcaaata atactaaaaa
    10561 actttttaaa gataaatcaa cttttagaaa tattccatat ctattttttt caacaaaaga
    10621 ggaaatagga tttgcaatag gaaaatctcc aagagcagtt gtaggaataa aagatgaaaa
    10681 tttttctaaa aagataattg aactaataga aatttaaaat aaaatagggg gtgatcttgt
    10741 gtcaaaaaca agagtatacc aaatagcaga agaattaaat atatcaaatg aagagcttat
    10801 aaataagtta gctgaattag atataaatgt aactgacaaa gattcagtat tagaaggtga
    10861 agagttagaa ttagcacttg aaatgttagg tgaagattta tctcaagaaa atggaaatgt
    10921 tatagaaata gatggaaagt tgacagtaca agtattagca acgaaattag ataaatctcc
    10981 atcagaaata attatgaagc ttatgaaaat gggaactatg gcaacaataa atcaagagat
    11041 aagttttgag attgctgctt tggctgccaa agactatgga tttgaattaa cagtagctga
    11101 aagtgatgat acagaagctt tagaaataga agctttaatg gaaattgaag aagataaaga
    11161 agaggattta aaacctagac caccagttgt tacagttatg ggacatgttg accatggtaa
    11221 gacatcttta ctagatgcaa taagaaaaac tgatgtgatt tcaggtgaag ctggaggaat
    11281 aactcagcat ataggtgctt cagaagttaa aatcaatgga cacaaaatag tatttttaga
    11341 tactccagga catgaggctt tcacatcaat gagagcaaga ggagcacaag ttacagatat
    11401 agcaatatta gtagttgcag cagatgatgg tataatgcct caaacagttg aagctataaa
    11461 ccatgctaaa gcagcaggtg tacctttaat agttgctatt aataaaatag ataaaccagg
    11521 tgcaaaccca gataaggtta aacaggaatt agcagaccaa ggattattag ttgaagactg
    11581 gggtggagaa gtaatagcag ttccagtatc agctaagaaa aaagaaggaa tagatacact
    11641 tttagaaatg gtattattgg ttgctgaaat ggaagagtta agagctaatc caaataaaag
    11701 agcagtagga actgttatag aagctgagct tgataaagga agagggccag ttgcaacagt
    11761 acttgttcaa ggtggtactt taacagtagg tgacccaatc gttgcaggtg ttgcatgtgg
    11821 taaagttaga gcaatgataa atgcaaaagg taagagagta aaaacagcag gaccatctac
    11881 agcagttgag atattaggtc tatcagaagt tccacaagga ggagaccaat ttgtcgaagt
    11941 tccaactgat aagattgcta gaagtgtagc agcaagaaga caacaaatag ttagagatga
    12001 aatgttaaaa tcaactcaaa gattatcact agatgctcta tttagtcaaa tgagtgaagg
    12061 ttctataaaa gaccttaata tagttataaa agcagatgta caaggttcag tacaggcagt
    12121 taaacaatct ttagaaaaat tatcaaatga agaagttcaa gttaaagtaa tacatggtgg
    12181 tgttggagct gtaacagaat cagacatatt acttgcagca gcttctaacg caataataat
    12241 aggattcaat gtaagaccag ttccaggtgc agaatctcta ggagaaaaag aaaatgttga
    12301 tataagaaca tatactataa tatacaaggc aatagaagat atacaagcgg ctatgactgg
    12361 tatgttagac cctgaatatg ttgatgaaga aacaggaaaa gctgaaataa gagaaatata
    12421 taaaatatct ggtgttggaa cagtagctgg atgttatgtg actaatggta agatatttag
    12481 aaattgtaaa gtaagattag ttagagatag cataataata catgaaggtg agcttgcagc
    12541 acttaagaga tttaaagatg atgtaaaaga agttaattct ggatacgaat gtggtatgag
    12601 ttttgtaaat tataacgaca taaaagaagg cgatatagta gaagcttata taactaaaga
    12661 agtagaaaga aaattataaa ttcgtatcaa agaaaatcag ttagtaaaga aggtcgatgg
    12721 tatggcatca tataatagaa caagaaggat agcagaagaa ataagaaaag tcgtaagtac
    12781 tatgcttata aatggagtaa aagaccctag aataacatct atggtgagtg taactgatgt
    12841 agaagttaca aatgacctaa gatatgctta tgtatatgta agtattctag gtggagatga
    12901 agaatccaca ctaacaggtc ttaaaagtgc tggaggatat ataagaagag aagtaggaaa
    12961 aaatataaaa cttagatata ttcctgagat agtatttaaa cttgatgatt caatagaaaa
    13021 aggaatgtat atggataacc ttataaaaag agttaatgaa aaaaatgctc aacaaaatga
    13081 ggatgaaaat tatgatgaat aatatggata gtattataaa ctatatttat gagggtgact
    13141 attttatagt tacctctcat atcagtccag atggagacaa tgttggttct acactttcaa
    13201 tgtattatac attgaagaat ttaggaaaaa atgtatatta tgtacttgat gatgaagctc
    13261 cattaaattt aaggtttttg gttgaaggtg taaaaatcta taagtctaat gaatttaata
    13321 tgaagaacta ttctttaatt gcacttgatt gtggggataa gatgagaata tgtgtgagtg
    13381 atgaaatcaa aaataatgca tcaaaattaa tctgtattga ccatcatgct agtaatgatt
    13441 catatggaga tttaaattat gtagatattg atgcatcttc tacatgtgaa ctagtttata
    13501 atttattggt tagatttcag caaacaaaag atattaatat tataaatgaa gatatagcaa
    13561 catgtttata tacaggtctt gtaactgata ctggaaactt ttcgtattca aatgtacatg
    13621 caagtagttt tgaaatggca aaaaatttac ttgttttagg tgcacaaaaa aatactataa
    13681 tacaaaatat atatcaaagc aattcttctg attattataa attattagga gaagctctaa
    13741 aaggtttgga gatatttgat tcaaaggtat ctagtatagt tttaactcaa gacatgatga
    13801 atagaaataa tattagcttt aatgatgttg atggaataac aacatataca agagatataa
    13861 aaggtataga agttggaatt ttatttaaag agaaaaaaca aaatgaaatt aaagtgagtt
    13921 ttaggtcaaa aaattatgtt gatgtaagtg aaatagccaa gttatttggc ggtggtggtc
    13981 atgtaagggc tgctggatgt actattagag atagtattga taatgcaaag aaaatggtac
    14041 tagaggcagt gttaaaatca atttaaggtg acagtaaaat gaataagatt ataagtatat
    14101 tgaaacccac aggtatgaca tctcatgatg ttgtaagtag agtgagaaag attttaaata
    14161 taaaaaaagt tggtcataca ggtactcttg acccagatgc ttcaggtgtt ttaccaatct
    14221 gtatagggaa agctacaaaa gtatgtgaag ttattttaaa caaagataaa tcgtatatat
    14281 gtgagcttac tttaggtata agtacagata cttatgatgc ttcaggtgaa attttaaaaa
    14341 aagttgatga ttttaaattt agtaatgaag acatagaaag agcttttgat acacaaagag
    14401 gagagataaa tcaacttcct ccaatttatt cagctttaaa ggtaaatgga aagagaatgt
    14461 gtgatttagt aagaagtggt agacaatctg aaattacttt aaaaacaaga agagtaaata
    14521 taaaagacat aaaaatacta agtataaaag gtaataaagt aatgttttat gttgagtgtt
    14581 ctaaagggac ttatgtgaga agtatttgcc atgatatagg agaatatctg ggttgtggag
    14641 ctcatatgtc gtttttgaat agaacatctt ctggtaaatt tgacttagat aattcaataa
    14701 cattagaaga attagaatta ttttatgaaa ataaaacttt agacaaatac ttatatgata
    14761 tagattatgt ccttgattcg tttaattatg ttgtattaaa ccctaatgct ataaaatatt
    14821 atagtaatgg tggctcaata gatgataaaa ggtttttaaa aaataatttc gataaagatg
    14881 atgaagttgt aagagtatac agtacagata atttcttagg attaggaaag ttatcaaaac
    14941 acaataatac aataagtgtt aaaagtgata agatgttcat ataaccaatg tatttaaaaa
    15001 aacaacaaaa gagatagtac tataataact tctatagtct atctctttta cttatgctat
    15061 aatagtttgt gaataagtta agagtaaatt atattttagc ggggaacatt tattatggtg
    15121 attataaaat ctataaaaga aatagcaaac ataaaagaaa gtgtcattac cataggaaat
    15181 tttgatggtg tacacaaagg acatcaagtt cttataggaa aaacagttga atatgcaaaa
    15241 aaagaaaata tacagagtat tgtatttaca tttgcaaatc atcctgtaaa ttactttaga
    15301 ccaaactcaa caaaaaatat tataagcaat gatgataaaa taaaattatt agaagatttg
    15361 ggaatagata tagttgtaat cataccattt gatgagtata tgactaaaat acctgcaaag
    15421 gatttcgtag atgagatact agtaaaaaaa ttaaaagcta aaaagatagt aattggacat
    15481 gactttacat ttgcaagggc aaaagaaggc aatgttaatc tattaaaaag cttagaacat
    15541 gaatttggtt ttgaggtaga agtaataaaa cccatcaaaa tcaatgatgt aagagtgagt
    15601 agtacttata ttagaagttt agtatcccag ggtgatatgg ctaatgtcaa ggaatatttg
    15661 ggtagaaatt acaagttaga aggttgtgtt atacattcta aacaccttgg aagaactata
    15721 ggattcccaa cggcaaatat tgaccttaag aataatatgc tcgttccaaa gagaggtatt
    15781 tatgcttcta ttgtacatat tggagatgag acttattttg gtgctacaaa tgtaggctat
    15841 aacccaacag ttaatggaaa aactttgtct attgaaacaa atattttaga gtttgataga
    15901 gatatttatg gggaaaatat aattgttgaa tttttggaaa gaataagaga tgaaagaaag
    15961 tttaattcaa tagatgactt aaaaaatcaa ttgtataatg atacaaatta tgtgtatgaa
    16021 aactatgttt gcaaaaacaa gtaatgtatg ataaaatgat taaggaaaat aaccattact
    16081 cggcatttga atactccaat caatgcttag taataggcgt ttctaaaaaa atacggaggt
    16141 aatctaaatg ataaacaaat cagaaataat aaaagaatat ggaagagctg aaggggatac
    16201 tggttctcca gaagtacaaa tagctttatt aacagctaga ataaacgagt taaatggaca
    16261 tttaaaaact cataagaaag accaccattc aagaagagga ctattaaaaa tggttggtag
    16321 aagaagaaac ttattagctt acttaaagga aaaagatctt gaaggatata gagcattaat
    16381 agctaaatta ggattaagaa agtaatttca ttctttataa aaaagcaggt atagtgtata
    16441 cctgcttttt tatagttaat tcattataaa gtaaaatagt taataataaa agtagtttat
    16501 atgttataaa aaattataaa atgaataata ttgggtaaga atattaaaat agtatacaaa
    16561 tacataatca atttaaaagg aaagacagga taaatataga aattttataa caggataatt
    16621 agttggaata aggaggatat tattttgaaa ataaagttag tatgcgatag tttgtgcgat
    16681 attccagatg aaatatcaga aaaagattat atagagatag ttccacttac agttattttt
    16741 aatgataggg aatatataga aggagtagat ataaagaaag aagagtttta taaaaaagta
    16801 aaagaaatca agcaaatacc taaaacttca caagctacat atatggagtt taaagaagtg
    16861 tttgataagt tcataacaga aggttatcat ataatctgta tgacaggggc tgcaaacgca
    16921 tcaggaactt ttcaaagtgc tatgatagct aaaaatgatg taaatgaaaa tgaaaaaata
    16981 catatatttg atactagaaa tttatctcta ggtagtggac aatatgttat aaaggcatgt
    17041 gaacttcttg aagaaggact gggatttgaa gaaattatag atgaattaga aaatactcgc
    17101 agtagtgtaa gattactttt tgcaccatat acacttgatt ttttaaagca gagtggaaga
    17161 gttccagttg caactgcttt gataggaaat atgttaaata taaagcctat atttttcttt
    17221 gataatgggg aagctaagtt ggtaaataag gttagaggaa taaagaatat agcaagtaaa
    17281 ctggtcgata taattttaga aatgaatgaa gggtctttag aaggaaaaat cgtcacaata
    17341 ggttgtggag ataatttaca cgattgtgaa atattaaaag atgaagtaaa taaaagaata
    17401 aaagcaagaa gagtattatt tactcgagga ggagtttcta tttgctctca tacagggcca
    17461 gatatattgg ctataagttg ttctaattag tagtaatata aggtttttgc atatatatgt
    17521 gaagataatt ttatattgtt aatttatagt atttttgata atatagtaaa tagagtgata
    17581 tacgtgtttt ttaataagag gaggacaagc attaatgttt gaacataaaa tatttaaaat
    17641 ggattttgca ggaagagaac tttctgtaga aataggaaag atttgtgaaa tggctagtgg
    17701 tagttgtata gttcgataca gtgacagtat ggtaatggta aatacaacta aatcagccaa
    17761 acctagagac ggaatagact tttttccttt aagtgtggat tatgaagaaa aattatactc
    17821 tgtaggtaag atacctggag gttttttaaa gagagaaggg aagccttcag aaaaagctat
    17881 acttacatca agattgatag atagaccaat aagaccttta ttcccaaaag gatttagaaa
    17941 tgatgttcag gtagttgcaa cagttctatc agtagaccaa gattgtacac cagatatagt
    18001 agcaatgata ggttcttcaa tagccctttc tatatcagac ataccattta atggaccaac
    18061 aggttcagta tgtgttggcc ttgtagatgg tgcatttgtt gtaaatccaa acgcagagca
    18121 aagagaaaaa agttcaatgc atctagttgt ttcaggtaca aaggaagcaa taatgatggt
    18181 tgaagccgga gctgatgaag ttccagatga ggtaatgctt gatgctattt tatttgctca
    18241 ccaagaaata aaaaaaatag ttgagtttat agaaggtata gttgcagaag ttggaaaaga
    18301 aaaaatgcct gttgaattat accatgcagg agaagaaata actcaattgg ttcgcgaatt
    18361 tgcaactgat aagatgaaaa aagcagttca gacttttgaa aagttagaga gaatggaaaa
    18421 tatggataga gttaaagaag aaactttagc tcactttgaa gaaactttag aagattttga
    18481 agattttgtt ggagatatag aagaagtatt acaagatata ataaaagaag aagtaagaaa
    18541 acttatagta catgaaaatg taagaccaga taatagaaaa cttgaagaaa taagacctat
    18601 atggtgtgaa actggcatga tacctagagc tcatggttca gccatattta ctagaggaca
    18661 aactcaagta cttaatgttg ctactttagg tgctttagga gatgttcaaa aattagatgg
    18721 gttagatgaa gaagaaaaca agagatatat gcatcattat aatttccccg catatagtgt
    18781 tggagaagct agaccatcaa gaggaccagg aagaagagaa ataggacatg gtgctttagc
    18841 agaaagagct ttattaccag taataccatc tcaagaagaa tttccttatg ctattagatt
    18901 agtttctgaa gtattaagtt caaatggttc tacatctcaa gcaagtgtat gtggctcaac
    18961 attatcttta cttgatgcag gtgtacctat aaaagatatg gttgcaggta tagcaatggg
    19021 acttataaag catgatggta aggttgcagt gctttcagat atacaaggta tggaagacca
    19081 tttaggcgat atggacttta aagtagctgg aacagagtat ggtataacag caatacaaat
    19141 ggatataaaa attgatggta tagataaaga aattctacaa cgtgctttaa aacaagctaa
    19201 agagggtaga atccatatac taggagagat gagaaaaact atatctcagc caaaaccaga
    19261 actttcacca tatgcaccta agattgttaa aatgcaaata aatcctgaca agataaaaga
    19321 tgtaataggg cctggtggaa agataataac taagataata gacgaaactg gagttaagat
    19381 agatattgaa caaactgggg aagtatttat atcaggtatt gagattgata tgattaagaa
    19441 agctcaagaa cttataaata atatagtagt tgaaccagaa gtaggaaaaa cttataaagg
    19501 taaggtaagt agaataatga actttggagc atttgttgaa atacttccag gaaaagaagg
    19561 attacttcat atatctcata tagctcatga aagagttgcc aaagttgaag atgtattgaa
    19621 tataggggat gaagttgaag ttaaagttac agagattgat gaaaaaggaa gagttaattt
    19681 atcaagaaaa gttcttttac caaaaccaga acataaaaat aaataactta ttatataatt
    19741 aaaagcaaag tcttaggatt ttgctttttt aaatacaaaa aagtagataa ttttaaaatg
    19801 atagttatgg atggcttata gttctttttc tgtaaggctt tactcttata aagaagctat
    19861 ttaaaagctg attaatagaa agctttattg atatatatag aatcacttaa gttatattta
    19921 gtgcataaac agagcttatt tttaatagta ttaaataaga aacaatttgt acataattag
    19981 ggggagtttt aatgataatg atggtactta ataagagtaa gctcaaaaaa atcataatac
    20041 taattttagt agtgttatta gtattatcag gaggggtaat ggtattaaag ttatttaaca
    20101 acaagccagt atttaattta tttaatggtg tagatttacc atatgaaaga ggtacaaagg
    20161 ataaaagtgg atatgttgca ttaacctgta atatagacct tggatgggaa acagagtacg
    20221 tagaatccat tttagaaaca cttaaaaaag aaaatgtaaa gattacattt aatgtaactg
    20281 gaaaatgggc agaaaagaat aaggatgaat tattaaagat aaaaaaacaa ggacatgaaa
    20341 taggtaatca tggatataaa catttagatt attctacatt atcatatgaa gacaattatg
    20401 agcaaataga gacctctaaa aagataattg aagaaattat aggtgaaaaa actaaatttt
    20461 ttcaagcacc agcaggttcc tttggtccag aaacagtaaa ggctgctaaa gcattagggt
    20521 atacttctat caaatgggat gccgatacta tagattggaa atacaaagac caaccagagg
    20581 taatcattga tagaatgaag aaaaaagaca ttaaagatag tagtataatc ttaatgcatc
    20641 caaccaacgc aacgacaaaa tgtatagatg atattatagc tattgtaaga gaaaaaggac
    20701 taaaaccagg gaaacttagt gatgtattta agtagacaaa gtataaatat ttggttaaga
    20761 ttgtataagt tgtagttgaa ttaattaaaa gactgactta taatgtaata catataaaaa
    20821 ataaatataa ttaaaacata gtgttttcaa taatccaata tgaaacaaag aataaattgt
    20881 taggagaatg taatgtataa gacaaaaata ctggaaaatg ggcttacaat aattggtgaa
    20941 gaaataccat atctaaagtc aataacatta ggtatttgga taaatgcagg ctctaggata
    21001 gaagaagcac aggtgagtgg aacttctcat tttatagagc atatgatgtt taaaggaacc
    21061 aaaaacagaa catcaaaaga gattgctagt tcaatagata atttgggtgg tcaaataaat
    21121 gcatttacaa gtaaagaatg tacatgttat tatgtaaaac taatagatga gcatatagat
    21181 acgggaattg atgtattgag tgatatgata ctaaattcta aatttgataa aaatgatata
    21241 gataaggaaa gacttataat attggaagaa cttaagatgt atgaagactc tccagatgac
    21301 ttatcttatg atttattagt ggaaaatata tatgctaatg atggattagg aatgaatata
    21361 attgggacaa aagaatctct atacaatata actagagaat ccatgttaga atacttaaat
    21421 aagtattata ttccaaataa cgctgttatt tctatagctg gaaactttaa ctttgatgat
    21481 atggtggaga aaatcaaatc aaaatttgga cactgggaaa agaaaaattt gagtatagac
    21541 ataagtgagg ctaagtttaa tccttgcttt atatcaaaaa ataaagatac tgaacaggtg
    21601 aacttagcta tctgcctaaa aggtattcct tttgaaaatg atgaagaagt ctactcaatg
    21661 gcagtggtaa ataatatttt cggtggaagt ataagctcaa ggctttttca gaaaataagg
    21721 gaagaaaaag gtttagtata ttcaatatac tcatcacaga cattatatag gaaatgtggt
    21781 gaattaggca tatttgcaag tatgagtaca gaaaatttac aagatgtata taatttaata
    21841 aagaaagaaa ttgaaaatat aagagaaaac tatcttacag aaaaagaaat atctgaaagc
    21901 aaggaacagt taaaaggaaa ttatatattg gatttagaaa gtactagcag tagaatgatg
    21961 tctactggta aatctatgtt acttagcaaa aaagtaaaaa ctacagatga aatacttgaa
    22021 tgtataaata atgttaatat taattcaata aagaaggttg ttgataaggt attcaatatc
    22081 gaaaatattg gtacatgtat agttggaaga gatgtagaaa agattctaca cttggattaa
    22141 tattgaaaaa agtaaatctc tggaggtata aaatatgaat ttatctgaaa tagcaggtaa
    22201 ggaaatagtt aacttggtca caggagaaag attgggggtc attggagaat gtgaccttat
    22261 actagatgaa actacaggcg gtatattagc gcttctaatt ccaaaggaaa aaggattttt
    22321 tgccagaaga aaagataagt catttttaga agtaccatgg agaaatgtaa aaaaaatagg
    22381 taatgacatg ataattatag aatatgaggg atatatttag gaggaaaaaa tgagtatttt
    22441 agtgcaaaag tttggtggga catcagtaga atcatatgaa aaaatgaatg aagtatgtaa
    22501 gatagtaaaa gcatacaaaa aaaatgatga agaattacaa ttagtattag tagtttcagc
    22561 aatgggaaga aaaggagctc catatgctac agatacactt ataaatttgt gtagtgcagt
    22621 aaatgatgag ccaagtaaaa gagaacttga tttaataatg tcttgtggag aaataatatc
    22681 aggaacaatt cttgctaatt tattgaatgc acaggggata gattctgttt tctttactgg
    22741 tcagcaagca ggtataatta caagtgatga atattctaat gcaaagataa agtatataaa
    22801 tcctaaaaag gtaaaaagag ctttagatga tggtaaggtt gtagttatag ctggattcca
    22861 gggtgtaaca gatgatggag aaataactac tcttggaaga ggaggaagcg atacatcagc
    22921 agttgctatt ggtaaagctt tagaatgtga aacagttgaa atttacactg atgtggatgg
    22981 tataatgact gcagacccta gagtagaacc aaacgctaaa gtattgagtt ttatagatta
    23041 tgaggaagta ttccaaatgg ctgacaaagg tgcaaaagta atacatccaa gagcagtaca
    23101 gttggctaaa agtggaaata taactttagc cataaagaat acaatgaatc caacttttga
    23161 aggtacaaaa ataggttctt tatgtagaca cttagaagag aatatagaat atgaacaaga
    23221 aagagatttt aaggttgctg ttgcaaataa agatagtgta gcacaagtta agattaaatc
    23281 agcagaagaa gtgtttacag aagtattaaa tgagatagaa aagaaactta taactataga
    23341 tatgataaat ttctttatat cagaaaaagc atttgttgta gaagatgctg atataaaatc
    23401 attaaaggaa atactagata aatttgaatt agattatgaa gtaaatagag actgtgctaa
    23461 ggtaacattg atatgttcaa gaattgctga tgaaatgtct ggtatcatgt caaaggtagt
    23521 tagaggatta tcaaaagcag gggtatctct tttacaaaca tcagactcaa atatgactat
    23581 atcttgttta gttagtgaag aagatatgca tacagcagta catgctatac accaacaatt
    23641 ttatttaaaa taaactgtat aatacaatta ttttttaatc aaatttaaga atttcatata
    23701 tatcttaata tatttgattt atttggtaaa actaaattaa atataatttt gataggagtt
    23761 tttttatgaa agattataga gaagataatg aaagatttga taaagataat tgtagtgaag
    23821 aatctagttg cgaagagaga gaatgtataa agcaatttgg taccaatgaa atgccacctc
    23881 aacctcctaa agatattcaa tgtataacta taattggaga aattgaggga cattttatag
    23941 gaaatcctca aaaaaaagct acaaagtacg agcatataat tccaatgcta tactcaattg
    24001 aggaaagcaa tgatgttaaa ggagtgttag ttgttttaaa cactgttgga ggagatattg
    24061 aagcaggttt agctattgca gaacttttaa acagtacatc taaaaaagta gttactcttg
    24121 ttctaggagg aagtcatagt ataggtgttc ctcttgctac agcaggagat tactcattta
    24181 tagcaccaac agcaaccatg attatacatc cagtgcgaac tactggtctt gtgattggta
    24241 taaatgagac ttttgaatat tttaaaaaga tgcaagatag aataattcaa tttataataa
    24301 gaacatcaaa tatcaaaaaa gatgtacttg aaaaacttat gcatgaaaaa gatgaacttg
    24361 taagtgatgt tggtagtgta ttaataggaa aagaagctgt ggattatggt cttatagatg
    24421 aagttggagg actaaaagaa gcacttaaaa aattaagaga gcttataaaa gaatctgagg
    24481 aagaaaaaaa taatgattaa aatttgttta aataataaaa tttatgatat aatgatgtaa
    24541 taaagtagga gtgttagtgt taaggcatta gcactttttg ctgtgaaggg gtaaaacttg
    24601 ctaaaaataa ggagtgatat taatggccac gaaaaagaag aaaaaaaaga aggtaaatac
    24661 cttaagtttt aatgcagaat atcataattt aataacaata tttataggtg tatttttatt
    24721 atatagttta aattctaatt ctatgggatg gatacctgtg ttaatgcaaa atctttttaa
    24781 gggactattt ggagggcttt caatagcaat accatttata gtaattataa caggactttt
    24841 aggatttttt gatggaaatg aatatatata taggctaaga aagactaaac tatactacat
    24901 aattatttta tttatatttg tattttatgg attattaaat gcaggaactc ttcctgtaga
    24961 tagcccacta aaaggtaaca tgtttgatga tgttatgaaa ttgggtgtaa gtggacaagg
    25021 tagtggtttg atagcaacaa ctattgcgta ttatatgagt aaaatatttg gtatagcagg
    25081 tggatggtta ataagtatct tcgcacttat actttctgta atgtttatat ttaatatttc
    25141 tataaaagat ttggtgtcaa atgcaaaatc aaaatctaaa gcatctaaag acagcaattt
    25201 aacgtttaaa gataaaattg caaatatgaa aaaatctgca atagatatga tgactgatga
    25261 agtagatgat actactataa ataaaaaacc aggtttcttt aaagggttaa tgtcaaaagg
    25321 tcttaataat gatgatgaag aagatgaata ttttgaagaa gaaaatagtg atggtgtaga
    25381 tgataaaact ataaaaatag ttggatttaa taaggcagaa gatgagtatt tagaaatatt
    25441 agaaggaact caaagtatgc cagagttaga tgttttaaaa gaattacaaa aagctaccaa
    25501 tgaaaatcca gttgtagata ctaaaccaga aaaaaaagtt gatatagcta aatctaattt
    25561 aaatatagaa aaaactcaac ctatgagtat tgtagcagag ccagttaatg aggattattc
    25621 aaactataaa aagcctagca ttgagctttt aaataaagta aataagaagt ctgatgaaaa
    25681 tggtaaaaag aaagtgttga aaaatgcttc tcttttagaa aagacactat cagattttgg
    25741 agtggaagca aaaattaatc aagttacagt tggacctact ataacaagat atgaaataca
    25801 accaagtcca ggagtcaagg taagtaaaat tgtaaattta actgatgata tagctttaag
    25861 tttagcagct aaaagtataa gaattgaagc cccaattcct ggtaaaagtg caattggaat
    25921 agaagttcca aatgaagaag ctcaaatggt tggtgtgaga gaagttctag aaagtgaaga
    25981 atttaataat tttaactctc ctcttgcaat gggacttgga aaagacgtag caggaaaaat
    26041 tataattgga gatataggta agatgccaca cttactaata gctggttcta caggttcagg
    26101 aaagagtgtg tgtgtaaata cattaataag tagtatttta tataaggcaa atcctgatga
    26161 agtaaaatta ctattaatag acccaaaagt agttgaactt gcaaattata atggaatacc
    26221 acatctattg ataccagtag ttactgaccc taaaaaagct gcaaatgctt taaattgggc
    26281 agttacagag atgaatagaa ggtataaatt atttgcagat gctcaagtaa aagatgtaac
    26341 aagttataat gagaaagcgg aagaaaaact tcctaaaata gtaataataa tagatgagtt
    26401 agcagattta atgatggcaa gtgcaaatga tgtagaagat tatatttgta gattagctca
    26461 aatggcaaga gctgctggaa tgcacttgat tgtagcaact caaagaccat ctgttgacgt
    26521 tataacaggg gtaataaagg ctaatatacc atctagaata gcatttgcag tatcatctca
    26581 aactgattca agaacaatcc ttgatatggg tggtgcagag aagcttctag gtaaaggtga
    26641 tatgttattc tatccacttg gtgcagcaaa accagttaga cttcaagggg cattcatatc
    26701 agagagtgag tcggaaaaag taattgattt tgtaaaaagt caggttaaag atggaataaa
    26761 atatgaagaa gatataattg aaactatatc taaggttaat acttcaaaga gtagtgatga
    26821 ggatgagttt ttgagtgaag cgatagaatt tgtcgtggaa agtggtcaag catctgcctc
    26881 tatgctacag agaagattta aaataggatt caatagagca gctagactta tagattctat
    26941 ggaagaaaga ggcataattg gagcaagtga gggaagtaaa cctagaaagg tattaataag
    27001 caaacaggat ttgcagaatt tagaaggtga atagaattgt cagttataaa aatagaaaaa
    27061 gcacttaagc tagataaagt aatatttgta gatgtgagaa ctgaaggaga atatgaagaa
    27121 gaccatatat tgaatgcaat caatatgcct ctttttaaaa ataatgaaca taatgaagtt
    27181 ggaacaattt ataagatgca gggaaaacat gaagcaatac aaaaagggtt tgactatgta
    27241 tcgtataaat taaaggatat atatttacaa gcagcagaac ttgcattaaa ttatgataat
    27301 attgtcatat attgtgcaag aggcggaatg agaagtggaa gtatagtaaa tttattatca
    27361 tcattaggtg taaatgtata tcaacttgaa ggtggatata aggcttatag aaattttgta
    27421 ttagagtatc taaggcatgt aatggataca aaaaactttg tatctgttca tggattgact
    27481 ggtacaggaa agactgattt gttacattta ttagatgaaa aaggaataga tgtactggat
    27541 ttagaaggca tagctaaaaa cagtggttct acctttggtt ttataacttt tgataaaaag
    27601 ccaccatctc aaaagaactt tgaaacaaaa ctatttgaaa gtgtattttt ttcgaaagag
    27661 aattatatat ttgtcgagag tgaaagcaaa cgagttggaa gtgttacagt tccaaatgaa
    27721 attttcgagg caatgacacg agaaggacat catatattat tagaatgtga tattgaaaat
    27781 agagttgata gactttgtag agattatatt tatgataaag atgaaggtaa tatattgatt
    27841 ttaaaagaat gtataaacaa gtttagaaaa agattgggta ataaagtagt agatgattat
    27901 attgatttat tagaatcagg tgaatataaa gaacttgtaa aaagatatct tttagaatac
    27961 tacgacccac tttatatgca ttcagtagag aaatataagt atagtaagat tataaatttt
    28021 aatgatacac aaaaagcttt agaagatgtt atagacttat atgagagtat attggaagga
    28081 gaaagtgaat gttaaaaata gcattggaat cattaggatg ctcaaaaaat ttagtagatg
    28141 cagaaataat gatgggaatt ttaaataaca aaggctataa attaattgga gattttgaag
    28201 aagctgatgt aattatagta aatacatgtg ggtttataga gtcagctaaa caagaatcta
    28261 tagatacgat aataaacttt tcagagttaa aaaaaactgg caatttaaag ttgttgatag
    28321 taacagggtg tctagcccaa agatattctg aagaattaaa aactgaaatt cctgaaatag
    28381 atgctatagt aggaacagga agctatcaaa atatagataa aatacttaaa gaattaagtg
    28441 aaatgcatca aattgtaagc cttaatgata tagagtttgt atttaatgaa gatttaccaa
    28501 gatatatatc tacacctagc tatatggcct atttaaaaat tggagaagga tgctccaata
    28561 attgtactta ttgtataata ccaaaactaa gaggtaaata tagaagtaga aaatttgaag
    28621 atataattaa agaagctaag aaattagctg aatctggagt aaaagaactt gtggttatag
    28681 cacaagatac gacaaagtat ggttttgatt tatatggaaa agaaagatta tcagaacttt
    28741 tagaagaact tgcaaaaata gatggattta agtggataag ggttatgtat tcatatccag
    28801 aatctataac agaggaattg atacaagtta taaagaaata tgataatata tgtagttact
    28861 ttgatatgcc aatacagcat gcaagtaaca atattttaaa attaatgaat agaaaaacta
    28921 ctaaagaaga tatattaaat aaaatcaatc tgataagaag taatatacca gatgctatac
    28981 ttagaactac gataatagta gggtttcctg gtgaaacaga agatgacttc aaacaacttg
    29041 tggactttgt tgaagaggtt aaatttgata gacttggtgc atttgcatat tcaagagaag
    29101 aagatacacc agcagataga ttgccaaacc atatagatga agaagtaaaa atacaaagaa
    29161 gagatacttt aatgatgatt caacaaaaaa tatctgaaga acttaatgac aaaaagatag
    29221 gaaaaactta tgaggtgtta atagaagaac agatagaaga taatgtatat acaggaagaa
    29281 cacaaggtga tgcggaagaa attgacagta tagtgtatgt aaaaagtgta gacaatcttg
    29341 aagtaggaga atttgtaagt gttcaaataa atgatgcaat ggaatacgat ttaatgggag
    29401 atgttttata tgaacttgcc taacaagtta actttgttta gaatattttt aataccagta
    29461 tttgttttaa ttatgttgct caatgtacca aataaatttt taatagcttg tattattttt
    29521 ataatagctt caattactga tgctttagat ggaaaaatag ctagaaaata caatctagtc
    29581 actgactttg gaaaatttat ggacccatta gcagataagt tattggttat ttcagcactt
    29641 acatgtatga tagaagacca tcttgtaagt agttggatgg taattataat agtggctaga
    29701 gagcttacag ttagcatatt aagagctata gctgctgctg atggaaaagt aattgctgct
    29761 ggaaacagtg gaaaacttaa aacgataact caaatggtat ctatagtgtt tttattgtta
    29821 ggcgctcagt ttgaaaatgt attgatatta aatattggtg aaattctcat tcttatagct
    29881 acactactta cattatattc tggatgggaa tatctatata aaaataaaga gctttttatg
    29941 tcttctaagt aaaaaattat gctttaataa ataaaaacta atgattaata aataaaaaat
    30001 aacaacttaa aattttaaaa atgtaaaaaa gggtatacta ttatagtata cctcttttca
    30061 ttttaagaat attttctttt gtgtatataa ttattaataa tggtttataa ataaaatgta
    30121 atgaataaaa atataatcat tagtagcgtt tttaaattga ctaggtttga tttatgttat
    30181 ataattaata tataacaaga gaacaaatgt ttgtagataa atgaagggat ggtatagaga
    30241 tgagtgtaga tcaagaaaaa ttaaaagcgt tgaatgaagc tttaggtaaa attgaaaaag
    30301 attttggtaa aggttcagta atgaaattgg gagaagcaac atctatgtct atagatgtta
    30361 tatcaacagg agcgattggt ttagacatag ctattggtat aggaggtcta cctagaggga
    30421 gaatagttga agtatatggt ccagaatctt ctggtaagac tactgttgcg cttagttgtg
    30481 tagcatcagc tcaaaaagat ggaggaatag ctgcatttat agatgcagaa catgcacttg
    30541 acccagtata tgcaaaagct ttgggtgtgg atgttgataa cctaataata tctcaaccag
    30601 atacaggtga acaggcttta gagatagcag aggcattgat aagaagtgga gcgatagata
    30661 taatagtaat agactcagta gcagcattag ttccaaaggc tgaaatagat ggagatatgg
    30721 gtgattctca tgtaggttta caagctagac ttatgtcaca agcacttaga aagttaactg
    30781 gttcaattaa aaaatcaaat tgtgttgcta tatttataaa ccagttaaga gagaaagtag
    30841 gaataatgtt tggaaaccca gaaactacta ctggaggacg tgcactaaaa ttctattcat
    30901 cagttagatt ggatgttaga aaaatagata caataaaaca aggtgataaa gttataggta
    30961 gtagaactag agttaaagtt gttaaaaaca aagtagcacc accatttaag caagctgaat
    31021 ttgatataat gtatggagaa gggatttcaa aaattggaga ccttttagat atagctgctg
    31081 atgtagatat agttaaaaaa tcaggttcat ggtatagtta caatgatact aaacttggac
    31141 aaggaagaga aaatgttaaa aaattcttgg aagataattt agatttaact actgaaatag
    31201 atgagaaagt tagagcattt tacaatttaa atgaagaaca tgaagaatca ggtacttcag
    31261 tatcaaaaga aattgtagaa gaataagtaa aaatacaaaa aaataactag taattaaaga
    31321 catgccacgc tccctattag cttgacacaa tgttgaaaaa attataaaat taagtgagga
    31381 taaaatatct aaatttaatt ttaattatta ggaatatttt ttaatcatag tgatgacatt
    31441 ttgggattat taacgaagga ggtggatgtc atagatagtg tagtaatgat tgtaataggt
    31501 gcagctgttg gtattatagc tggatatttt gttagaaaaa atatatctga agcaaaaatt
    31561 ggacaagctg aaaacttggc caaagaaata atagataaag cacaccacga ctcagaaaca
    31621 gtacaaaaag aaaaattact agaagctaaa gaagagatcc ataagtggag aacagaagct
    31681 gaaagagaaa acagagaaag aagaacagaa gtacaaaagt atgaaaaaag agtagtacaa
    31741 aaagaagaag tcttagatag aaaattacaa aatttagaat ctaaagaagt aaacctaagt
    31801 gaaaaattaa aggtagtgga aaagaaagaa gaagaagtcg aagtaataaa aactcaacag
    31861 ttagaaaaat tagaaagtat atcaggaatt acttctgata aggccaaaga aatcatctta
    31921 accaatgcgg agagagatgt aagacgtgaa atgtctataa tgataaaaga gatagaaagt
    31981 caagctaaag aagaagctga taagaaatca agagaaatta taggatatgc aattcaaaaa
    32041 tgtgcagcag atcacgtagc cgaaactact gtaacagtag taagcttgcc taatgatgaa
    32101 atgaaaggta gaataatagg tagggaaggt agaaatataa ggactttaga aaccttaaca
    32161 ggtatagacc ttataataga tgacactcca gaagccgtaa ttttatcagg atttgatcca
    32221 ataagaagag aaattgcaag aattgctcta gaaaaattaa tagcagatgg aagaatacat
    32281 ccagctagaa ttgaagaaat ggttgaaaag gcgagaaaag aagttgacaa tattataaaa
    32341 gaatatggtg aacaagctgc atttgaaact agtgtacatg ggattcatcc ggaattaata
    32401 agattattag gtagacttaa ctatagaact agttatggac aaaatgtact taaacattct
    32461 atagaagttg ctcatatagc tggtataatg gctgctgaaa taggagcaga tattagattg
    32521 gctaagaggg caggtttatt acatgatata ggtaaggctg tagaccatga aatggaaggt
    32581 acacatgttg aaataggtat ggatttactt agacgttata aagagtctaa agaagttata
    32641 catgccatga gtactcatca tggagattac gaacctcaaa cagtagaggc tgtattagtt
    32701 acagcagcag atgcaatatc agctgctaga cctggtgcga gaagagaaac tttagaagca
    32761 tatataaaga gacttgaaaa attagaagaa atagctaatt catatgaagg tgtagaaaaa
    32821 tcatttgcta tacaagctgg tagagaaata agaataatgg taaaaccaga agctataaat
    32881 gatgaagaaa tacatctatt ggctagagat atgactaaga aaatagagga tgaacttgaa
    32941 tatcctggac aaataaaagt aagcataata agagaaacaa gagctataga gtatgcgaag
    33001 taaaagaaat aatatctaaa taataagaaa atatttttag aaatttaaat ttgccacaag
    33061 atatgataat atatcttgtg gcttttaaat tgtttaagtt taattttata taatgcaaaa
    33121 ataatctaag tgctaaaagt gctacttaca aaagattaat aaaataaagc tttatatatg
    33181 tattatttat ataattatcc aagtttgttt gatataatga agttgtaaaa tcaatacttt
    33241 actacaaaat aaatagtaaa tactacagt
//
LOCUS       DAEPLF010000023        32194 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEPLF010000023 DAEPLF010000000
VERSION     DAEPLF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32194
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.023"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..806)
                     /locus_tag="KR314_000961"
     CDS             complement(<1..806)
                     /locus_tag="KR314_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0327359.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTFVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLE"
     gene            complement(890..1423)
                     /locus_tag="KR314_000962"
     CDS             complement(890..1423)
                     /locus_tag="KR314_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH0327360.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGLNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(1550..3625)
                     /locus_tag="KR314_000963"
     CDS             complement(1550..3625)
                     /locus_tag="KR314_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0327361.1"
                     /translation="MLKGLSFRNARRQAGKYLIYWCSLIGSVALIYAFNALIFSDVMQ
                     ELSSILNNGGSDEFSYMIIIFSFIITFILGWFVSYMMNFILKKRSREISTYMILGVDK
                     KQIVKMLFIENLLLGASALIVGWILGIFVSKVLESIVINMFHGKYSLSPDFSLKAAGM
                     TFLYFCIIYLIAQLKSNKKLVKMKLINLLNYDRYNENVLIHQTKTKSIMFCVSVLCGG
                     MGIFFFSIPSGRLTDVFLGLLLIVFCLIVLFMGFGSIAQNMFEKGMNWKYKKSHLFLY
                     RLLASKINGISIIMGIIATLFTVSIACIGIANSFYSVMNKSVDLQQFDVSIFHPNESY
                     DFSSYEGYLSETKGVKKDYSYTLYTEEKTSFMKVRNNILSKYLKDIGRDLQYKDYTFA
                     ENQFDTFMKYSDYNILRNMLGLNPIKMDKNQFIIHCMPYLADAYKNYIGEKYKLTISD
                     KDLSIAGIYIENFSQYGGYGNGQEYIIVIPDYLIDNMKPLYSLYVAITSEPLDSAYFT
                     EFQKKFDTLENLNSGVVVAGNQGFVSKLRYDNKDYISGKYAMQSTGQAIILILPLFYL
                     ALIICIIGTVILSIQLLSENNIIIKHYGMLRTLGMIDSEVIKILKKHVCLYFSIPLIP
                     AVIFGGILVSVFSYNMFNISFDAPVFFNIQIPILHAVAITLINFVVIYCIYVFITYVA
                     LRKEILHIK"
     gene            complement(3628..4374)
                     /locus_tag="KR314_000964"
     CDS             complement(3628..4374)
                     /locus_tag="KR314_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327362.1"
                     /translation="MLLRVENIEKYYGNQKILTKALNDVSFQINTGEFVSIMGTSGSG
                     KTTLLNCIATIDTVSAGKIYLENQDITELSEENLTVFRRDNLGFVFQDSNLLDTLTLE
                     ENIALPLTIKKVSPVEITNSVYNIATQLKIEEILSKFPYEVSGGQRQRCAVARAVVTE
                     PKLILADEPTGALDSNSSKTLMELLETINTILEATILLVTHDAISASYTERILFLRDG
                     KIFNELRKGEKSRLTFYHEILDALSLMGGD"
     gene            complement(4388..4783)
                     /locus_tag="KR314_000965"
     CDS             complement(4388..4783)
                     /locus_tag="KR314_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBH0327363.1"
                     /translation="MKKRILTILCVVLVLFISITGLDSMGFFSRSIIPLRSEYASFIR
                     KNGTITEVKFTGPFNHNNIDLNKNVEDTNKNGDDNELTIKYDIEVKKSLISSNEPTIQ
                     IQDSKESIYVYILNFSDKTIIIKNGKIVD"
     gene            complement(4896..5918)
                     /locus_tag="KR314_000966"
     CDS             complement(4896..5918)
                     /locus_tag="KR314_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0327364.1"
                     /translation="MRFCNFLQDKVLVILLNICCAISLVLYLHLIGIGQGELTLLIAA
                     WVAILFCWICFCYQKQKKEIIKLRRIVDELDKKYLFIEMIEKPTSNIEREYYNIIRLS
                     LKSMTDHVSQIHREMYEYRQFIEQWVHEIKHPVTTSELICENYKNEHTRKIRFQMEEI
                     ERHIERALYYARLGHVEKDYLISEITLKSIVEEVLAKNKLLLIQNNIHINIQDLEYSV
                     YTDKKWIVFILNQIIINSVQYKSDSAEISFFAIKEDKSVKFTIKDNGIGIKESEIYRI
                     FELGFTGSNGRTTNNSTGIGLYLCYELSKKMGIDIDVSSSLGEYTAITLNFPINTAIN
                     MQKQCN"
     gene            complement(5915..6586)
                     /locus_tag="KR314_000967"
     CDS             complement(5915..6586)
                     /locus_tag="KR314_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0327365.1"
                     /translation="MILTNILIIEDDVFICKELMQFFENNGYSTQTLDTNNCDVLQVI
                     QESTPDLILLDINLPNIDGFSLCRQIRNCSTIPIIFITARDSAIDELNGLTMGGDDYI
                     TKPYNLPLLLARVKNLLKRVDSSDKQKIKYREIILNLISATVEYQNAVIELSRTELKI
                     MYYLFQNVGSIVPRIDLVEYLWDNHIHIDDNALSVNITRLREKLRGIGITDLIITKRG
                     MGYRI"
     gene            complement(6672..8021)
                     /locus_tag="KR314_000968"
     CDS             complement(6672..8021)
                     /locus_tag="KR314_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBH0327366.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNRANSIEEIKPCEISKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIDKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEVIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(8033..9415)
                     /locus_tag="KR314_000969"
     CDS             complement(8033..9415)
                     /locus_tag="KR314_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBH0327367.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            9659..10873
                     /locus_tag="KR314_000970"
     CDS             9659..10873
                     /locus_tag="KR314_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBH0327368.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIKLYGNKCDYAKRDTLLYTANKLEIKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            11050..12963
                     /locus_tag="KR314_000971"
     CDS             11050..12963
                     /locus_tag="KR314_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0327369.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDNIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            13234..14208
                     /locus_tag="KR314_000972"
     CDS             13234..14208
                     /locus_tag="KR314_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBH0327370.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            14229..14504
                     /locus_tag="KR314_000973"
     CDS             14229..14504
                     /locus_tag="KR314_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH0327371.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            14517..15680
                     /locus_tag="KR314_000974"
     CDS             14517..15680
                     /locus_tag="KR314_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH0327372.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGTEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            16017..18296
                     /locus_tag="KR314_000975"
     CDS             16017..18296
                     /locus_tag="KR314_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0327373.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVNFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSVVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPMPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(18391..19731)
                     /locus_tag="KR314_000976"
     CDS             complement(18391..19731)
                     /locus_tag="KR314_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0327374.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILIGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVTYYFIR
                     GKSTLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     IVQVFPEVFVGIFNKDSDLMDIAVRGISINLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(19743..20525)
                     /locus_tag="KR314_000977"
     CDS             complement(19743..20525)
                     /locus_tag="KR314_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327375.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(20540..21562)
                     /locus_tag="KR314_000978"
     CDS             complement(20540..21562)
                     /locus_tag="KR314_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBH0327376.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(21568..22581)
                     /locus_tag="KR314_000979"
     CDS             complement(21568..22581)
                     /locus_tag="KR314_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBH0327377.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(22709..23647)
                     /locus_tag="KR314_000980"
     CDS             complement(22709..23647)
                     /locus_tag="KR314_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0327378.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWSQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            23917..24381
                     /locus_tag="KR314_000981"
     CDS             23917..24381
                     /locus_tag="KR314_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327379.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTGKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(24493..24810)
                     /locus_tag="KR314_000982"
     CDS             complement(24493..24810)
                     /locus_tag="KR314_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0327380.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(24823..25554)
                     /gene="chbG"
                     /locus_tag="KR314_000983"
     CDS             complement(24823..25554)
                     /gene="chbG"
                     /locus_tag="KR314_000983"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBH0327381.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVVEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(25580..26890)
                     /locus_tag="KR314_000984"
     CDS             complement(25580..26890)
                     /locus_tag="KR314_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBH0327382.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNNGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLVAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(26967..28280)
                     /locus_tag="KR314_000985"
     CDS             complement(26967..28280)
                     /locus_tag="KR314_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0327383.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(28302..28613)
                     /locus_tag="KR314_000986"
     CDS             complement(28302..28613)
                     /locus_tag="KR314_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0327384.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(28813..29517)
                     /locus_tag="KR314_000987"
     CDS             complement(28813..29517)
                     /locus_tag="KR314_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0327385.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(29612..30973)
                     /locus_tag="KR314_000988"
     CDS             complement(29612..30973)
                     /locus_tag="KR314_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0327386.1"
                     /translation="MKDRINLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAIYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     MSE"
     gene            31472..>32194
                     /locus_tag="KR314_000989"
     CDS             31472..>32194
                     /locus_tag="KR314_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0327387.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRN"
BASE COUNT        11329 a         5331 c         4090 g        11444 t
ORIGIN      
        1 tcctctaaaa actgtaaaaa ttcctgatta aatagtttaa gctcatcata gaatatagca
       61 tgaccactat actcaaaagt atataaaatt gaatcttcta ttttttcatt cataaaaact
      121 gcaaattcat acggacatat ttcatctaat ttcccatgaa atataccagt aggcactctt
      181 acacatttta aatcattaaa taattcttca tctcttaaag atacagcagt tccaatagtg
      241 ccaatacctg atgcactaaa accaatatca ttaaaccatc ttctaaagct ttctggtgga
      301 tttgatgcaa atactttttc tccaaaatcg gttaccattt ctggtctatc agtacatgct
      361 tgtgctatca atttatttac atcttcacgt gtcataccat atggaaattc tggaggtctt
      421 tgtacaaatg atggtgcagc agctgcagct aataccaatt ttgacactcc atatccatta
      481 aataaactca tatacctaag tacgattgct cctcccattg aaaaaccaac aaaggtaaag
      541 tcttttaatc caatagcatg taccacctta taaatatcgt ctgctaattg gctataagta
      601 tatcctcctg atgttgcatc agatttacca aaacctctta aatcaattga aactgttcta
      661 tatccgagtt ttggcaatat atttgtttga tattcaaata tcttatgtcc taagggccaa
      721 ccatgtataa aaagtacagt ttttttagca ctcggattca aatcatatac tgctatgttt
      781 atatcattca catatacata atacatatca acagcaactc ccttcaagaa aatgtatgat
      841 ggttatttta tatatatgaa taaaatgatg aataactaaa tttttaaatt tatttacaat
      901 acttctctat tgccttttca aataaactta catgaagttt ttcatctagg attattctat
      961 ttaatatagc ttttatataa gggtcttcaa taagacttat atctttataa taattattaa
     1021 tagccacatg ctccaaatgc aaatctagtt ttaatctgtt acaaatagaa tttccataac
     1081 atacaaaacc accattccaa tatgcaccac aagaacttgt agaacctcta tatacaggat
     1141 ttccacccaa taatcttata gtctttgcta atatctccat atgtaacatt tctgttatag
     1201 atatatttat ccacatttca cttaactctc tatgcgtttc atctaaatca aaattatggt
     1261 atagatattg atttacactc gtaaattcac ttgatactcc ggcatagtca tccatcaata
     1321 attctgcata atacttattt agacctaaaa cttttatctc tggatatggc tcatcacttg
     1381 aatatccatt tcgcttgtgc atgtcgtaat gtgcatcact cataatatct cctcctttaa
     1441 atttaatact tgatattaat tgtatgcatc acgacttttt catataactt agataataaa
     1501 atctattata ttattttcta ttataattta tgtgtcaatt cacccaaatt tatttgatat
     1561 gcaatatttc tttccttagt gctacatatg taataaaaac atatatacaa tatattacta
     1621 caaaattaat aagcgtaatg gctacagcat gtaagatagg aatctgaata ttaaaaaata
     1681 ctggagcatc aaaagaaata ttaaacatgt tgtatgaaaa aactgatacc aatattccac
     1741 caaatattac tgcaggaatt aatggaatcg aaaaatataa acaaacatgt ttctttaaga
     1801 tttttattac ttcactatca atcatcccca aagttcgaag cattccatag tgttttatga
     1861 taatgttatt ttcgctcaat aattgaatag agagaatgac tgtaccaata atgcatataa
     1921 tgagtgccaa ataaaaaagt ggtaatatca agataatggc ttgaccagta gattgcatag
     1981 catactttcc acttatataa tccttattat catatcgtaa tttagaaaca aagccttgat
     2041 tacctgctac tactactcca gaattaagat tttctaaagt atcaaacttt ttctgaaact
     2101 cagtaaaata agcactatcc aaaggctcac ttgtaatagc aacataaaga ctatacagag
     2161 gtttcatatt atcaattaaa taatctggaa tgacaataat atattcttgt ccatttccat
     2221 atcctccata ctgtgaaaag ttctctatat aaatacctgc aatacttaag tccttatctg
     2281 atattgtcag tttatatttt tctccaatat aattcttata tgcatcagca agataaggca
     2341 tacaatggat gataaattga tttttgtcca tctttattgg gtttaaaccc aacatatttc
     2401 ttagtatatt gtaatcacta tatttcataa atgtgtcaaa ctggttttct gcaaaagtat
     2461 aatccttata ttgtaaatct cgccctatat ctttcaagta tttagaaagg atattattac
     2521 gaaccttcat aaaggatgtt ttttcttctg tgtaaagagt ataagaataa tcctttttca
     2581 cacctttcgt ttctgataaa tatccttcat atgaagaaaa atcatatgat tcattcgggt
     2641 gaaaaatact cacatcaaat tgttgtaaat ccactgattt attcatcaca gaataaaatg
     2701 agttagctat tccaatacaa gcaattgata ctgtaaaaag agttgcgata atacccatta
     2761 taatgcttat tccatttatc tttgaagcca gtagacgata caaaaataag tgagatttct
     2821 tatatttcca attcatccct ttttcaaaca tattctgtgc aatagatcca aatcccataa
     2881 ataatacgat tagacaaaat acaatcaaca ataaaccaag aaatacatct gttaatctac
     2941 cactaggtat tgaaaagaaa aaaataccca tacctccaca caaaacagac acacaaaaca
     3001 taatagactt cgtttttgtt tggtgaatta atacattttc attatatcga tcataattaa
     3061 gcaaattaat taatttcatt ttaacaagtt ttttattact ttttaattga gcaattaaat
     3121 atattatgca gaagtataaa aaagtcatcc cagcagcttt taatgaaaaa tccggagata
     3181 aagaatactt tccatgaaac atattaataa caatagattc caaaacctta gaaacaaata
     3241 tccctagaat ccatccaaca ataagagcag aagcacctaa taaaagattc tctataaaaa
     3301 gcattttaac aatttgtttt ttatctactc ctaatatcat ataagtactt atttctctgc
     3361 ttcttttttt caatataaaa ttcatcatat aactaacaaa ccagcctaaa ataaaagtaa
     3421 taatgaacga aaatatgata atcatataag aaaactcatc gcttcctcca ttattaagta
     3481 tactagaaag ttcttgcatt acgtcggaaa atatcaaagc gttaaaagca taaatcaaag
     3541 ctacagagcc tattaaggaa caccaataaa taaggtactt tcctgcttgt cgtcttgcat
     3601 tacgaaaaga taatcctttc aacataatca atctcctccc atcaaagaaa gtgcatccaa
     3661 aatttcgtga tagaaagtaa gtctggattt ttctcctttt ctaagttcgt taaaaatttt
     3721 cccatccctt agaaataaaa ttctctcagt ataactagct gaaatagcgt cgtgtgtgac
     3781 aagaagaatc gtagcctcta atatggtgtt tatagtttcc aaaagttcca ttaaagtttt
     3841 agatgaatta gaatccaatg caccagttgg ttcatctgcc aaaataagtt tgggctctgt
     3901 tacaactgca cgagctactg cacaacgttg cctctgacca ccagatactt cataaggaaa
     3961 cttcgaaagt atctcttcaa ttttcaattg tgtggcaata ttatacacac tattagtaat
     4021 ttcaacagga gaaacttttt ttattgtaag tggaagtgct atattttctt ctaatgtcag
     4081 agtatctaat aaattagaat cctgaaaaac aaaacctaaa ttatcacgac gaaaaacagt
     4141 taagttttct tcggatagtt cagtaatatc ttgattttca agataaattt ttccagcact
     4201 tactgtatca attgtagcaa tacaatttaa caaagtagtt ttaccagagc cagaagttcc
     4261 cataatactg acaaactctc ctgtattaat ttgaaatgac acatcattca aggcctttgt
     4321 aagtattttc tgattgccat aatatttctc aatgttctct actcgtaata gcatgttaaa
     4381 gtcctcctta gtcaacaatt tttccatttt taattattat tgtcttatct gaaaaattca
     4441 agatatatac atatatgctt tctttagagt cttgaatttg aatagtaggt tcattagatg
     4501 aaattagaga tttttttact tcaatatcgt attttattgt taattcatta tcatctccat
     4561 ttttattagt atcttctaca tttttattta aatcaatgtt attatgatta aagggaccag
     4621 taaactttac ttctgtaata gttccatttt ttctaataaa agaagcatat tcagacctta
     4681 gtggtatgat acttcgtgag aaaaaaccca tcgaatccaa tcctgtaatt gaaataaaca
     4741 aaacgagtac aacacataat atagttaata tccttttttt catatgtttc tccctccttt
     4801 ctattgatat tgtagcgtaa atagtaatct gaatatatag aagttactta actttatctt
     4861 acagttttga aagataaaga aatagcagcg catttttagt tgcactgctt ctgcatattg
     4921 atagctgtat ttattgggaa attcaatgtt attgctgtgt attcaccaag gctggaggac
     4981 acatctatat caattcccat tttcttagac agttcataac aaaggtaaag accaatccct
     5041 gtagaattgt ttgtagtcct tccattactg ccagtaaaac ctaattcaaa aattcgataa
     5101 atttcacttt ctttgatgcc aataccatta tcttttatag taaacttaac actcttgtct
     5161 tcttttattg caaaaaatga aatttcagct gaatcacttt tgtactgaac actattgata
     5221 ataatttgat ttaaaataaa aactatccac tttttatcag tatatacact atactccaaa
     5281 tcctgtatat taatatgaat attattttgt ataagaagca atttattctt tgctaatact
     5341 tcttctacaa ttgactttaa tgttatttca gaaattaaat aatctttttc aacatggcca
     5401 aggcgagcat agtacaatgc acgctcaata tgacgttcta tttcttccat ttgaaaacga
     5461 atttttcgtg tatgttcatt tttataattt tcacatataa gttctgatgt tgtaacagga
     5521 tgttttatct catgtaccca ttgttcaatg aattgtctat attcgtacat ttcacgatgt
     5581 atttgagaaa catgatctgt catagatttc aatgataatc taataatatt gtagtattca
     5641 cgttctatgt tagaagtagg tttttcaatc atttcaataa acagatattt tttatccaat
     5701 tcgtctacaa tgcgacgtaa cttaataatt tcttttttct gtttttgata acaaaaacat
     5761 atccaacaaa ataaaattgc gacccaagcg gctataagaa gagtcaactc tccttgacca
     5821 atccctataa gatgaagata tagcactaat gaaattgcac aacaaatatt taagagtata
     5881 actaatactt tatcttgaag aaaattacaa aacctcatat cctatatccc atcccccgtt
     5941 ttgtaatgat taagtcagta atacctatac cccttaactt ttctctaagc cttgtaatgt
     6001 ttacacttaa agcattatca tcaatatgaa tatgattgtc ccataaatat tctaccaaat
     6061 ctatacgagg cacaatactc cctacattct gaaacaaata atacatgatt tttagttcag
     6121 ttcttgaaag ttctataaca gcattttggt attctactgt agctgaaata aggttgagta
     6181 taatctctct atatttaatt ttctgtttat ctgatgaatc tactctttta agtaggttct
     6241 ttacccttgc taaaagcaaa ggtaagttat atggttttgt aatatagtca tctcccccca
     6301 tagtcaaacc atttagttca tcaattgcac tatctcttgc tgtaataaaa ataataggta
     6361 tagtagaaca attcctaatc tgtctacata aagaaaaacc atctatatta ggcaaattga
     6421 tatccaagag aattaagtca ggtgttgatt cttggattac ttgaagtaca tcacaattat
     6481 ttgtatccaa tgtttgtgta ctatagccat tattttcaaa gaattgcatt aattccttac
     6541 agatgaacac atcatcttca attatcaaaa tattggttaa aataataaat acctcatttc
     6601 tttagtttat attgctttaa ttttgtaagt aatatatttt tccatatatt acttacaaaa
     6661 ttacttttta attattcatc ttttccagct actgatagct ctttaataac aactgaaggt
     6721 gaacctatac tactcattgg aaattctaaa tcttccccta taacttcaat atctttcaac
     6781 aggtcaaaat agtttcctgc tactgtaatt tgctccactg gaaatacttt ttttccttct
     6841 ttgatataaa agcctttggc agctaaagaa aagtctcctg ttactgaatt tgcacctgaa
     6901 tgaagccctg caaagctagt aaccatcaat ccttcatcta tatcctttat aatttcatct
     6961 aaagatttat ctcctttatc tatataaaaa ttagttggat atatgcttat aggtgatgaa
     7021 tatgaagatt ttattccatt tcctgtagtc tttacacctg ccttattagc cgtttttaag
     7081 ttatgaagta aagttataag ctttccattt aaaacaactt cttttttaaa tgtagctact
     7141 ccttcatcat caaaaggagt agatgccata ccattttcta aaagtgggtc atcaactatt
     7201 gtaactatag gagatgctat catgtctcct tctctatttt tcaataaaga taatcctttt
     7261 tgagctgaat ctgcattaaa tatcccagaa aatgtgctaa gcaaagatac catagcttca
     7321 ttaagcaata tagttttata tttacctgat ggaatactct taccacctac tttagataaa
     7381 gcattatcaa caccaagttt tgatatctca caaggtttta tttcttcaat agaatttgct
     7441 ctattatatc ctattccatc atatttttgt ccattgtctt ctatgatagg aactacaaac
     7501 cctataagca tattagtttt attgcttaaa ttaagacctt tagtattaga tataccatta
     7561 cttgatacag aataagatat agtacattta gataaattta ccaccttatc tgaataagct
     7621 ttagtctctc tttccatttc taaagctaaa tcaatcaact tatctgcctc taaattttct
     7681 agctctttag aataagtctt tacatcatta tagtgcttat ctccttcata tataaattgt
     7741 acatcttcat tttcaatagt gtttactcca tcttttacat tttttatcaa catatcaaca
     7801 gctttatcat ctaaaatttc tgtataagaa taacctatct ttccatttac cttacctcta
     7861 aaagataaac caaaagattt atcaagattg tatttctcaa cttctccttc atatatattt
     7921 atacttaaat tttctcctgt ataataatat atttcacact cttcaaaacc ttcacttaga
     7981 gcctttttaa ataatatatc tttaaaattt ttaagttcca caattatccc tcctatcttc
     8041 cacctacagt aatttctttt acccttatca taggttggcc tacattagtt ggtatacttc
     8101 ctgatgatga gccacacatc ccctgagctt gttttacgtt attcccaacc atatctatat
     8161 tcataagtat atcgctacct tttccaatta aactagcacc tctcactggc tcttgtattt
     8221 ctccattttt tattaagtaa ccttctgaaa ctgcaaaatt aaactctcct gttacaggat
     8281 ttacagaacc accacccatt tttttagcat aaagtccatc tggtattgac tttataatgt
     8341 cttctggtct atcttctcca gcagcaatat aagtgttagt catccttgaa gttggtgcaa
     8401 atttgtagtt ctgtctacga gaacttcctg taggattcat tcccattcgt ctaccattaa
     8461 gtttatctat catataagac ttcaaaattc cattttcaat cagtatattc ttttgagttg
     8521 gatttccttc atcatctata ttcatagaac cccagtagtt tggtattgta ccatcatcta
     8581 tagctgtaat ctttgtggaa gcaatctgtt gacctaattt atctgcaaat acagagttcc
     8641 cctttgcaac tgatgtagct tccaaagaat gtccacaagc ttcatgaaat attactccac
     8701 caaatccatt atctattgct accatcatat tacctgcagg acaattttta gcatgcaaca
     8761 tagtatgcgc tactctagct gattcttttg cataatattc tgggtctatt tcttcaaata
     8821 tttcaaaccc tttacaacct ccaggacctt caaatccagt ttgattttca ttacccttag
     8881 atgcaattga acttatccct agtctggttc ttattcttct atcctcaaca tatatacctt
     8941 cagtgtttgc aattaatatt ttttggtctt tatccaaata gctaacactt acttgagata
     9001 tatcattact aaattctttt gcactcttat atgcaacctt cataacactt actttatctc
     9061 tatttcctat tgaatttgga taaattttta tattatgtat gttatttatt ttaatactgt
     9121 catttaatat tacacttttt ccatcttcca atttacctaa agcaacagca gctttgtatg
     9181 ctacatccaa cagactattt aatgaattgt cacttgtata agcatatata ctcttcaatc
     9241 ctttaaatat tcttattcct ataccataat ttcttccacc tattgcattt tctactttat
     9301 tatcaattaa actaatagaa ttatttatag tgtcttcttc aaaaatctca gcaaaatctc
     9361 ctccagtaat tagacacttt tctaatacct tagaagctat attttttgaa agcataaatt
     9421 tacctcctac tttaaatttt taattaactt ttattcaatc tttttacttg ttaacaaaca
     9481 ttatatacta tattacttaa aataaggtaa ttatgaatat attatacata tgttttatac
     9541 taaaatgtta tcacatttta tctatacata cccatataac tagataatta aaatgtataa
     9601 tattatttgc tatagaaaat aatacactca tacataataa attttaggag tgaagtatat
     9661 gaatcaagaa tatgtaaaag gaaatcctat ttttactaat attaataaag ttccaagaca
     9721 atttccatac cttactgatg atattgatac tgatgttata attgttggtg gtggcgtaac
     9781 tggatgtatt tgtgcttact atctagctaa aaacaatata aagtctgtca ttcttgaaaa
     9841 aggaaggatt gcacatggaa gcactagcgt gacaacttct ctattacagt atgaattaga
     9901 tgacaatttg atagatttaa cagaggttat gactctaaaa gatgctctaa aagcatataa
     9961 cctttgtact tcagcacttg aagaattaga tacatttata aagctatatg gaaataagtg
    10021 tgattatgcc aaaagagata ctctacttta tactgccaac aaacttgaaa ttaaggctat
    10081 caaagaagaa tataacttaa gaaaagaaaa tggatttgat gttgaatata ttgatgaatc
    10141 cacaaaccct tttagctttg atttaaaatc aggattgata gcaaagaatg gaggacgaga
    10201 attagaccca tataaatata gtcaccattt gattgatgta agtttaaaaa atggtcttca
    10261 agtatatgaa aatacagaag taaaaaaagt agatttatca aatgataagg ttactgctga
    10321 agtatcgtat ggatacaaag ttcatggtaa aaaattaatt gtagctacag ggtacaatac
    10381 aagtttattt acaaaaagaa attttgcaac taagtcaaat acttttaata tagcaactaa
    10441 accacttaaa aacatagctt cttggaaaaa caacatttta attagagata actgtgaccc
    10501 atataattat ctaagaacta ctaaagacaa tcgattaatt ataggtggtg aagatgtaag
    10561 ttttgatgat atagaaaatg aaactttagc caatgaaaag tatgatattt tagaacaaag
    10621 attgaaaagt atgtttaagg atataaaaga cattgaagtt gaatataaat attgtggttg
    10681 ttttgcttca actttagata atttaggttt tattggtcca gataataaaa ataacaatct
    10741 atggtattgc ttaggttatg gagcaaatgg tatactcttt gcaatattag gaggtataat
    10801 gttaagtgaa ttatatcttg gcaaacaaaa taaaaatatg aaattattta aagtagatag
    10861 atttgataaa tagatattgt atattaaaag actataaaaa gatgaaactt tataattcta
    10921 aaagttaact ttgatacagt cttttataat gttcctttta aaaatacttt atttataatt
    10981 aaatataaag taaaatgtta aaatataact gataaaattt agtcataaga taatatttaa
    11041 gtttatttga tgaaatatgg aggaattatt atgaaagaaa tactcgttat tgctcccact
    11101 aaaggaacct atgaaaaatc aattcacata gttaaaaaaa acaaatatac aaatattgat
    11161 gttgtatttg gcaatctaaa agaaggtata cctcttgctg aaaaaagtat taatcatggt
    11221 actagaatta tcataagcag aggtggaact tacaatatgt taaaagctac ttacaatata
    11281 cctatagttg aaattaaagt agatgcttat gatattataa aaagttacaa agaggtaaaa
    11341 aattccaacg aaccatttgg aataattgga tttaataatg tcatctacgg tttcgatatt
    11401 atagaagaaa tattgaataa gaaaataact atgattgaaa ttgaaaaaga agaagaaatt
    11461 tatgatgcta tagaaaagta cagaaagaaa ggtataaaca catatatagg agataccact
    11521 gttgcacata ttgttaagag acttaattgt aagggaatat taatcgaatc tagagaagaa
    11581 aacatactta gagccattca acaagcagaa caaatactcg aagcaaccaa ggatgaacaa
    11641 aaaagaagat tacagataga agctatgaca gattttgtac atgatggaat aataacagtt
    11701 gataaaaatt ttaaaataac attatttaat aagagtgctg aaaaaatatt tggtataaaa
    11761 aaagaaaatg cactttatca taatgttgta gatgttatcc caaacacagt tcttccacaa
    11821 gtagttgaga ctatggaacc tcaaattggt gaaatacaaa atatagggag tacaaaaatt
    11881 attacaaata gagttccaat aattgttgat ggccagattc aaggggctgt ttcaacattt
    11941 caagatagca aggagataag ttcttttgaa catacaataa gaaggagctt attaagcaaa
    12001 ggtctatctg ctaaatacac ttttgataat ataattcatg ctagtgaaaa gacaaaagat
    12061 tgtatagaac ttgcaaaaaa atatgcttgt tatgatactc ctatgattat tactggagaa
    12121 tctggagttg gtaaagaatt attttgtcaa agtatacata actatagtaa gcgtaaaaat
    12181 gcaccttttg ttgctgtaaa ttgtgctgcc atacctccat ctctaataga gagtgagttt
    12241 tttggttatg aggaaggttc atttacaggt gctagaaaaa aaggtaaagc aggtgtattc
    12301 gaactagcac atgaaggtac aatcttccta gacgaaataa gtgaaatccc tcttgaactt
    12361 caaggtcgat tattgagggt attgcaggaa aaacaagtta tgagaattgg tggtgataaa
    12421 gttattccta tagatgtaaa aattatctgt gcttcaaata aagacttaaa aaatatgatg
    12481 cgcaatggtt tatttagaca agacctttat tttagaataa atatactaac attatatctt
    12541 ccttcattaa aagaaagaaa agaagatatt ataaaattat cagaattttt tatacataaa
    12601 tattcaagca aatacaataa aacgccttta attatcactc caaatattaa aaaggcactt
    12661 cttgaacgag aatatgaagg taatataagg gaattggaag gtatgatgga aagggctgtt
    12721 attgtggaat catttgactc gattttatta gaaaaggtta gcaatgaata tacaaataat
    12781 atagttgata aagataatag tgattttgaa tgcagtaatt tagatttaaa aactttagaa
    12841 aaaaaatata tctataaaat ttataacgaa aataatagaa atactacaaa aacctgtgaa
    12901 attctaaaga taactcgctc cactctatgg cgtaagttaa aagaaataga agatgatgtt
    12961 taaaaataat acatacctat atatatcaaa acgttttatg tttaatttta atacataagg
    13021 cgtttatttg ttttcaataa aaaatacaat tatatgataa aaagaatctt tgtaagtgaa
    13081 attctcatca atcatacctt taattataag taatctacag catttcaatc gattattaaa
    13141 aataatcatg ataactaaaa atacaaactc tatatattgg catagtaatt gcttttataa
    13201 tacctatata aaaatgaaag ggagtgatac tagatgcaca tcttaaaatc agtgaaaaag
    13261 ataccaggag gacttatggt ctgtcccttg ttattgggag cactatttaa tacagtatgt
    13321 ccacaagctc ttgaaattgg aggattcaca acatccttat ttaaaactgg tgcaatgagt
    13381 atacttgctc tattttgtct atgcaatgga gctcaaatca atatcaggca agctggtaag
    13441 ccattgacta aaggtatagt acttacagca acaaaattta taattggtgc agtattaggt
    13501 attattgtta gtaaatttat tggacctaaa ggtataattg gtattacacc tcttgccata
    13561 gttgctacca tgactaattc aaatggaggt ctatttgctg ctcttgcagg tgaatatggt
    13621 gactccacag atgttggagc aatatctatc ttatctctaa atgatggtcc tttttttaca
    13681 atgcttgctt ttggagtaac tggacttgca aatgtaccta tagtagcact atttgcagca
    13741 cttataccaa tactacttgg atttatactt ggtaaccttg atgaagatat acgagaattt
    13801 cttgcacctg gtacaacatt attaattcct ttcttcgctt ttcctttagg agcagcatta
    13861 aactttaatc aaattataac tgctggacta cctggagttg tacttggttt aggagttact
    13921 ataataacag gtatgggtgg ctactttgta atgaaactta tacgtgcaga acatccagca
    13981 gtaggtgcag ctacaggtac aacagccgga aatgcagctg gtactcctga agctttagca
    14041 gctatagacc ctactctagc tacaatagca gctgtttcaa cagtacaggt tgctgctgct
    14101 attattgtta cagcaatatg ttgtccaatg ttagtttcct atctagataa aagagaaaaa
    14161 aagaaaaaat ctatttttaa aacaaataaa aatattgaag taaattaata cttaaggagg
    14221 taaaattaat gaatgcaatt attcatgcaa atgaaaaaga taatctagtt acatgtgtca
    14281 gacctttagt taaaggtgaa aaagtttcaa ttgatggaaa gtcttacata gtaaatgata
    14341 atatacctgt ctttcataag atggctacag aagatgtaaa aaaaggtgat gttgtataca
    14401 aatacggtga aattatagga attgcaacag ttgacataaa aactggtgac tatgttcatg
    14461 tacataacat tgaaagtaca cgtggtcgtg gagataaaaa ataggaggaa gttaaaatga
    14521 aattaatggg ttatttaaga gaaaatggtc agtttggtat tcgtaatcat gttttaatta
    14581 ttccaacatc tgtatgttca agtgagacag ctacaagaat agcatcactt gtgcctggag
    14641 caatagcaat ccctcatcaa catggatgtt gtcaaattgg ttctgatata gagcttacag
    14701 caaagacatt aataggattt ggaaaaaatc ctaatgttgc cgcagtttta gtagttggtc
    14761 ttggatgtga tggtattcaa gcaaaagaat tagcatctga aatagctaca acaggaaaaa
    14821 aggtagatta cgttgttata caagaatgtg gtggaacatt aaaaacagtt tctaagggaa
    14881 ctgaaatagt aagtaaaatg gctcgtgaag tatcaaaaga agtaagagtt gaatttggta
    14941 tgagtgaaat tacattagca cttgaatgtg gtggttctga cccaacaagt ggaatagctt
    15001 caaacccttc aataggcgtt gcatcaaacc ttcttgtaga tgaaggtgga agtagcatac
    15061 taagtgagac tacagaagta attggtgcag aacatttgtt ggctacccgt tttgaagatg
    15121 aagaaatgaa agataagttt cttaaatttg taagtgatgt agaaaaaaga gcaattgcta
    15181 tgggagaaga tttacgtagt ggtcaaccta ctccaggtaa taaagctggt ggactttcaa
    15241 caattgaaga aaaatcactt gggtgtatgt ataaggcagg caataagcca ttcaaaggtg
    15301 ctcttgaata tgctgatatt gtacctcctg ataaaaaagg attatatttt atggatactc
    15361 caggtcaaga tatagattct atcactggta tggtagcagg tggtgcacaa attgtaatct
    15421 tttcaactgg tagaggtact ccaacaggaa gccctatttc accagttatt aaaatcactg
    15481 ggaatagtga tacatacaac aaaatgccag acaatatcga tataaatgca ggtagaatta
    15541 taacagatgg tgctaaaata gcagatatag gtcaagaaat atttaatgaa ataatagaag
    15601 tctgtaatgg taaacatacc aaagctgaaa gtcttggtca tagagaattt ggcatttaca
    15661 gaatttcatc tacattttag attgtaacaa aatatctttt ataaatataa tacttatgta
    15721 gatacataag ctaaaagttc tttctaatcc tataaaaatt ttctatactg aaaaggaact
    15781 gtatcaaaat tattttgaaa acagttcctt ttttaacctc ttttactcaa aatattataa
    15841 aataagattt aaacaataaa cgtgtcttga ttaatctcta agaagacata aataaagcta
    15901 aatgtaaagt gagataaata aaatttaata tctttgtatg aaaagatatt tggtataata
    15961 tgtctgagag aattcttatt tgtaaacaaa taaaggatgg aggatgaaac aaaagaatga
    16021 actttgaaaa cacaaaaaat aaaaaaataa tagctacttt actattgaca ctccttttaa
    16081 tcgtgtcatt aacatgtgtc aattttatta tgaatctaaa taaagtaaca cataatgaaa
    16141 cagaacgata tcttgtagaa gtttcacaaa gtatttccac tacaattaat gtgaaatgta
    16201 aaagtatatt taggacacta tattctatgg gagatactta ttttgagatt aatgatagtg
    16261 aaaaagaagt caatagatac ttatcaaaaa aattaaaatt atttgaattt gattggttag
    16321 gtattataga ttctaatgga aattttgtaa gtgcccttaa agatacaggt aatgttaaag
    16381 gacaagactt ttatagaaaa gcccttcaag gtaaagaaac aattactcct gttaaatcac
    16441 aatctggtac atatggaatt ttatattctg taccactata caatggcgat aaaattgttg
    16501 gagctgttgc tgcttggaat actattgata ctatgagaaa tttattaagt gtagaaagct
    16561 ttcatggtga aggattttcc caaattatcg accaagatgg aaattttatt gtaagttcag
    16621 caaacaaaaa tgctccaaaa aatgtggtaa atttttttga catgattaaa caccaaggaa
    16681 aacaggttga taaaaataaa cttgaagaaa tgaaaataaa cttaaaaaag aacaaaactg
    16741 gttctatgta ttatacatta aatgatggta tttctaaggc aatgcattat cttccattac
    16801 aagaagaaaa cttatacctc ctttcagttg ttccaacaaa aacagcaagt gcacagtttg
    16861 atttacttct aaaaaaagca atcttcatca atattttaat tgttatacta tttttattgc
    16921 ttattatatt gattatgttt ataaatcaaa agaacagggc acaacttata aaaatcgcct
    16981 atgtagactc tgtaactaaa ggttacagta aagcacgttt tgatattgaa gctaaaaaaa
    17041 ttatacagtc atcttcatca gaaagctatt cattggtatc tataaatatt cagaaattca
    17101 aacttattaa tgattctttt ggtagtgaag caggagataa aacattaaag tacatacatg
    17161 atgtcattag taattatcta gaaccaggag aattgctttg tcgagtttct aatgaccagt
    17221 ttaatgtact tattaaaact attcctcaaa aagaaattct taagtacata gaaaatatca
    17281 ccaatgatat taatagtttc aataaaaatg aagaatgtaa atattttata gctctatcta
    17341 taggtgtata taaaattgat gacccaaatc tacctttagt ttcaattcaa gaccgtgcaa
    17401 atgttgcacg aaaaaataat aaaggaaaca ttggaaatca tccactttat acatgtgttt
    17461 tttatagtga tttagagcgt ttaaaaatga ttaaagaaaa agaaatggaa aatagaatgg
    17521 aggaagcatt aaataataac gaatttatag tatatcttca gcctaaaata agcctcaaag
    17581 ataatactat tgtaggtgca gaagcattag taagatggca agacccaaat aaaggcctta
    17641 taccaccaga cgagtttatt cctttctttg aaaaaaatgg ttttattaca aaactcgacc
    17701 actatgtttt tgaaaaagta tgtagtatga taagaaaatg gatagataat ggagaaactc
    17761 caatgcctgt ttcagttaat ttttcacgtg ctaatttaaa taatatagat tttttagaaa
    17821 aatacagaat gcttcgtaat aaatacaaca ttcctgctaa gctcttagaa attgaattaa
    17881 ctgaaacact tgtatttgaa aatctccaaa aattaattaa tataatagac caaatccatg
    17941 aagaaggttt tcaatgttca ctagatgact ttggaagtgg atactcttca ctcaatatgt
    18001 taaaagaaat aaaagttgac acattaaaac ttgaccgtgc tttttttagc tcacctaatg
    18061 cagataatat tgctgaaaat catgtaatag aatctatagt taatttggca aaaaaattaa
    18121 atatgaatag tatatcagaa ggtgttgaaa caatcttgca aatggattat cttaaaaaaa
    18181 ttaattgtga tatgatacaa ggatatgttt tttcaaaacc tctgcctcaa gaaatatttg
    18241 agaaaatgac ttttggaaaa aaactagaag aaaccagtat ggatagttcc gtttaaataa
    18301 aataaaaaac ttgtctctat aaaataattt taatcatttt ataaagacaa gtctttttat
    18361 tttccaattt aattttattt tctaaatttc ttatactcgg cttgttttaa attccctttt
    18421 taagaataat aatacgatta tcatagcaat aaaatctgca actggctgtg ccaaccaaac
    18481 accatctaaa ttaaattgaa ttggaagtac aatgattaat ggtataaata aaataaattg
    18541 tcttaaaaga gttaaaaaca ttgaatgttt aactttactt atacactgaa aatacacagg
    18601 tcctacaata gaaatcccca ttataggcaa tgtaattaga tttatgctta tacctcttac
    18661 tgctatatcc atcaaatctg aatctttatt aaatatacct acaaatactt ctgggaaaac
    18721 ttgtactatt aaaaatccaa atgacaatat gacaactgac actatgatag ataatattaa
    18781 agttttcttt gcacgttcat attgttttgc accataattg tatgatatta tagtttgcat
    18841 accttgattc aatccaaaca caggcatcat aaacatcaat aaaatagatg taagtgctgt
    18901 catagcccca atagctaaat ctccaccata aacctttaga gaattgtttg taaacatgtg
    18961 agttatactt atagctactt ccatagcaaa aggtgtcatt cctattgcaa ataccttctt
    19021 tactatatct ttattcaatt ttaagttaca tttttttagt tttaaagtgg acttccctct
    19081 tataaaataa tatgttaccc aaaagaatac tactatttga catagtactg ttgctatagc
    19141 tgctcctttt atacctaaat taaacacaaa tataaatata gggtccaata tcaggtttaa
    19201 tacacatcct acaatcattg tccttgctgc taattttgga ttaccttctg ctctaattgc
    19261 attattaaga gaaaatgcaa ctaaattaaa tatacttcca atcaatatga cactcatata
    19321 gtcttttgca taaaatattg aatctttact tgcacccaaa ataaaaagta tatcttctaa
    19381 gaaaactaat cctaatatag ttataattaa agagataatt atagataatg tcaaagcatt
    19441 acctaatatc ttctctgctt cttctctatt tctttcccct aattttatag acaatcttgt
    19501 gctagcacct actgatacca acattccaaa agctattatt agagtaaata ctggcatagt
    19561 tacaccaaga cctgcaatag ctatagagcc aataccttcc atagaaccaa taaaagctct
    19621 atctactaca ttataaagtg aagttaccat cattgctatt attgccggta tcgaatacct
    19681 aaataataaa atccatattt tttcatgtct taatgcttct tgattctcca tgttgtcacc
    19741 atttacacca tgtcataagt tatacaaatt ggtttatttg ttcttgggtc tgttgctatt
    19801 tctgcatcaa tattaaataa ttcttttaga ttatccttcg tcataacatc atatgcatca
    19861 ccttcacaaa ctattttacc tttcttaata cctatcatgt ggtcagcaaa tcttgctgca
    19921 ttattaagtt catgtattac catgacaata gttcttcctt gttttttatt tagttcatct
    19981 aataatttta gtatctctaa ttgatgtgcc aaatctaaat atgttgtagg ctcatccagt
    20041 aataatatat cagtttcttg tgccaaagcc atagctatcc atgccctttg tctttgtcct
    20101 cctgataaat cttccatatt tctatcctta aattctgcaa ttccagtaac ttctaaactc
    20161 caatttacaa tctctctgtc ttctttacta gaattaccaa atccactttt atgaggaaat
    20221 cttccataag aaattaattc tgaaacggta agaccactag gagcttgagg gctttgtgga
    20281 agtacagcca tatcctttgc tatttcttta gacttctttt tgtagatgtc ctctccattt
    20341 atatatattt tcccactatt agggctaata attctaccta tagttttaag tattgtcgat
    20401 tttccacaac cattagcccc tatgatggta gttatttttc cttttggtat atttaaattt
    20461 aaatttttta taatatctgt gtttccatat gatacattta gattcttagt atttatacag
    20521 ttcataacaa caccctttcc taatcattta ccaacattaa ataaataaag tacggcactc
    20581 ctattataga aactactata cctactggta tttctatagg tgctagaaga tttcttgata
    20641 tagtatctgc tattaaaagt aataatgttc ctatcaatgc tgatactggt attaatcttt
    20701 tatgctttgg tcctattatt tttctaccta tatgtggagc tattaaacct aaaaatgaaa
    20761 tactacctgc tactgctgtc gatacaccag ataaaattac tgcatatatt ataagtcttc
    20821 ttctttgttt ttctacatct acaccaagac ttatagatac ttcatcaccc aaatttaaaa
    20881 catctatata tttagattta taaatagtta tacccataaa aatcactata aatggaagca
    20941 cagctattac atatttccaa ctggttcccc aaatgcttcc tgatgtccat gccataaccc
    21001 tattaaattc ttgtgttgta aatttcaatt ggaatatcgt caaaattgaa gtaaaagcta
    21061 tgtttacacc tataccaata agtagtaatc ttgacgaatt tataccacct ttccatgcaa
    21121 gagaatatat aagaaatgct ccaaataaag ctcctataag tgcaacaata ggcattgtaa
    21181 atacagtcaa gttactcata ccttcatata cattaccatt cataaggtat atataaacta
    21241 ctacaaacaa tgcagcacct gaatttattc ccaaaattcc agaatcagct aaatcattct
    21301 ttgtaactcc ttgaagtatt acacctgctg ttgataatgc agaaccaacc aaaagtgcta
    21361 ttactattct tggtaatctt aatttaaata ttgcaatttc atgattacgc tgaccctgcc
    21421 ctaaaaaagt ctttataaca tccattggtt ctatagaaaa tgagcccata tttaaactca
    21481 ataaaaaagt gataaaaata agcaccatta aaattgatat aacaagtaat gttttagaat
    21541 ttatttttat aatatttttc atagtaccta tttctccccc tttctaacaa ggtatataaa
    21601 tattggcact cctataagtg ctgttataga acctactggt gtctcatatg gaggatttat
    21661 catcctactt aatatatctg tatatacaag caatacagct cctaatacta aagagctcgg
    21721 tattatatac ttataatctg aacctactat tcctttagct atttgaggaa ctataagacc
    21781 tataaaaata atattaccag acactgacac agatgctcca gtcattaata ttacaagtac
    21841 tatacatata aatctaacaa agttagtttt ttgtcctaat cctattgcca cttcttctcc
    21901 aagacttaat atagttattc ttggagccat aataatagct aaaataaatc caataccacc
    21961 agctacaaat agcaatttta taccttccca cttcactcct acaagccctc ctgatatcca
    22021 gaaacttaat tgttgagata ggttaaaata catagatatt gctgatgcca aagaaattag
    22081 caatgtacca agagcgactc ctgcaagagc taattttaca ttattaactc ttcctcttga
    22141 tttagaactt ataaaatata taaataatcc acttatactt gctccaataa atgcaaacat
    22201 cattacacta aaacttccat gtataagttg aggaagtttt ttttgaagta ccagtgatat
    22261 cgaaattgcg aatgtagctc cttgtgtaat tcctataaca gatggttcag ctataggatt
    22321 tctcgttatt ccttgcatta ttgccccaga aactgctaaa aaccctccaa caagtgcagc
    22381 tgctattgct cttggaagtc taacatcctt tactattctc atatttattc catcttccat
    22441 ttttatcaaa ctatctatga cagttgatag attcatatct tttgccccaa tagttatgga
    22501 taatacaatt ccaaatacaa gtacaactat acttactaat atcaacaaat aagttatatt
    22561 ttttttactc agcttagtca attatcctac ctccttttcc cttttaatcg tcagtataat
    22621 atcatctatt aagtatttta attttaagat ttattaaaaa taggtgtgaa aaatgaatat
    22681 cattatctca cacctactaa gttaagaatt actttgttaa ttcattttta actgattcta
    22741 ataataactc ttttccaatt ggattgtatg attgactgaa aaatggagca gcatcaagta
    22801 ttgttacatt accatctttt actgctctta tttgagacca gatagcacta ttttctaaat
    22861 cttttttatc tgcttctgta gcaattacaa ctatatggtc tgcatctata tctgttaaac
    22921 cttccatact taccattggt agtgttattc catcctgttt tggcatattc ttaggtctag
    22981 ctaatttcat atcatcattg attagagtac ctattccacc atcactaaat accataaatt
    23041 gacctgaact tgccaacact ggtaaataag ttttttctcc attcttttct ataacttcct
    23101 tacctaactt tgtagctttt tcatcatact tctgtagcca acttttagct tcttcttctt
    23161 tgtcaaataa ctttgaaaca tcagttaact tgcttctcca gtcatttgca taatctttca
    23221 tcataactac aggagctatt tcttttaatt ggtcatatat tttttcttgt ctttgactca
    23281 ttattattaa atcaggattt acttctaata tagcttccat atccatagta tccatcattg
    23341 agtgcccaac tatctttaca cctttaagct cttctcttat gtaacttggt aatttatcag
    23401 tttcatatga gtctacattt gctgttgcaa caggcttata acctgctaat aatagttctt
    23461 cactacttcc tgatatatca actatttttt taggatttga tggtatctta acctctcctt
    23521 taacagattg tacaactctt gtctctgatt tatctttatc ctctgtattc ttatctgaac
    23581 atgctgtaac aagtactaac gtagttataa tagatataaa tattgcaagt gattttattt
    23641 ttttcatgat tacttctcct acttgtttat tatacttaca tgatattgat tatcataagc
    23701 acatatttat tatagcattt ttctattttt agtcaatatt attttgtaaa aggataaaaa
    23761 ttcctaatta atcttaatta tatatagctt agataaggtt ttcttatatt aaacagtatt
    23821 ttttttaaga attggacaca aacacaatga cttttaggtt aaacaatgat aaaatgcaat
    23881 ttatagataa tgaatttagt aaggaggatt caaaacatgg tacatttagt atattgtgat
    23941 aatgcaggaa aaaaaggaga aaaggtctta gataaaatct tggctggaac aaaaacaatg
    24001 gttattagag gtgctgctgg tagaaaaata ccacatagta gagtatttga agatgagtta
    24061 ctttatttta tggaaaaagg aactggaaaa atatctgcaa aagccactgt taaaaaagta
    24121 gaaaactatg ttaaattaac agaagatgaa atttctaaaa ttttaaatga aaatcaacca
    24181 aaattaaatt tatctgaaaa gcaaaaagtt cgttggcata aaaaatgttt atgcttagtt
    24241 gaatttgaaa atgttgaaga aataacacct cttgattttg accatcaagg aaatatggat
    24301 gattggctta ttgttgaaaa aattgaagat gtagttgttg gtactagtat accttacaat
    24361 tatgagaagt ctaaatttta aagtatcaat aacataatac tatattgcta aactctactt
    24421 aaataaaaaa agagtgcatt taataattat aaaaattatt tttatacact cttttttaat
    24481 aataaattta aattacttac tttctaatct ttcatataca tctataaatt cttctgctaa
    24541 ttgttgaaaa ttcatagctg tcattaaatg gtcttgtgca tgaataagta taacacttat
    24601 ttcagtttta ttaccattag cttcattttg tatcaattct gtttggaatt tatgagcttt
    24661 atgaattgat tctttcgact tttcaataca ttctctagct ttatctatat tcccgctctt
    24721 tgcttctttt attgcttcaa atgctagccc tttcgcatca cctgcaaatc caattattcc
    24781 aaaagatatc tctatcatct tctcatccat ttattcgacc tcctaaatat ctctatagct
    24841 tgatatgact aatccattct tttctaaaaa ttctttgact tttttagatg taagtatctt
    24901 gtgctctttc gttctatcaa tagcataaga tgtagaattt aaaatataat catctaaaaa
    24961 agctgggtga gacataatat ccactacatc atacttcatt atttcgtcta tattttttat
    25021 aaaatattct tcacttacat tttccttata aaaactatct attaaaggaa ctactttaga
    25081 atactctaaa ttatattcaa atccacctct tattggaaga ttatacttgg ttacaatctt
    25141 ctctactact ggctttaaac ttactaaagt atggatatga tggtgactat ctaaatgaga
    25201 tatatctatt ccaagccctt ttaccctatc tatttgagca caaagttctc tataaatctc
    25261 atcacaatcc attttttcaa taatttcgtc tgtaatcctc ctaataaaaa aaccgtcttt
    25321 atcaacaata gtttttaagt tagataataa tggtctacca caacttaaag tcatatgaac
    25381 tccacaattt aaagttttat tttctttaag taagcctact ccatgttcta ccccaggcat
    25441 atttgccatc atagaagtag acctaactat accattatta tgagctgaaa ttattccata
    25501 gttcacagct tcacaatatc caaaatcatc tgcatttatt ataattttag tcataccata
    25561 acctactttc tatagacaac tatctattaa atctaggaag ataatcttta tgagcttcca
    25621 acatttcgtc taatatagtt tttgctaagt catcagatgg aatcaatggg tttatacaaa
    25681 gtgccaaaag agcagattca taatcgccat atacagctgc tttagctacc aatacttcaa
    25741 atgatttgat ttggcttact aatccatgca cagaatctgg taaatatcct atacttattg
    25801 gttttggacc atttttggtt attacactac tcacttctac cgcttgttca tctttaaaat
    25861 ctcttataga accattgttt aatgtattaa ctacttggat atcttttttg tcattgtata
    25921 tagaacttat taaattacaa gcagcatcac tatagtaagc ccctcctctt ttttcaagtt
    25981 gaggtggttt tacatctaat ttttcatcct tatataattc aaataattgt tcttccaatt
    26041 ctttaactac ttgacctcta gcttttccct ctttaaactc tctcaactca tcctcaagca
    26101 tttctttagt tttatagtag tatctatgat atggacaagg tattgcaccc aaagatttta
    26161 caaattcagc attaaaatca attgccttta tattttgcat acttatatct gctttgacaa
    26221 acttatcaat tgcttctttt gtaacatctt ctccatctaa agatacattt agaccataaa
    26281 ccatatgatt aagacctgca aaatccatac ttattctgtc actttctaca ttaaatagtt
    26341 tggcaacatc attttttaaa tgaattggca cattgcatag acctatatat cttctaaaat
    26401 tagtatattt gaatacagcc tctgttatta ttcctgttgg atttgtaaaa ttcaccatcc
    26461 atgcatttgg acataattct tccacatctt ttattatatc aaatataact ggtattgtgc
    26521 gaagggcttt aaataaacct ccagcaccat tagtttcctg ccccataact ccatgagata
    26581 aaggaatagt ttcatctttt attctcgcat ctaaaagacc tactctaagt tgtgttgtca
    26641 caaagtctgc atctcttaga gcttctcttc tatctaaagt aagatttatt ttcatatcta
    26701 ctccagcttt ttttaccatc ctcttagcta gatttccaac tatctctaat ttttcttttc
    26761 cagcttctat atctacaagc cataattcct ttacaggtaa ctcttcatat ctatttataa
    26821 aaccctctac aagctcagga gtataacttg aacctcctcc aattgtaact atttttagtc
    26881 cctttttcat ctcattatac catccttacc tataatttaa tatactctac aaaaccctat
    26941 acatttattt accgaaataa aactacctag ttgttaattt tcaatttatt agcttccatt
    27001 ttttcagacg ctatgacaaa tggaatatat attaatattg ataatattaa attaaatagt
    27061 gctaaagctc caccagtcca gtcacctgta gccatcgctc cacctaatat aggaggtgtt
    27121 acccaaggta ttttaggtaa aattactggt gctactaatc caaaatctat tgctaagtat
    27181 gatacagcac tacatatcac tggtgcaagt acaaatggaa tcataaatat aggatttaaa
    27241 actattggaa gtccgaatat caatggttct cctatattaa atattgcagg tggcccacct
    27301 aatactatca tttctttacg tcttctccca gctattaata aagctactat taatcccaat
    27361 accattccag aaccacctaa atatacaaat gcatcaaaga atgcacctgc aagtacatgg
    27421 tatccttccg ttgctcctgc ttgagccaag gcagcatttt ctccacctaa tttcataagt
    27481 atagtttctg taaaaggaag tgctatattt gcaccatgaa gtccaacagt ccaaagtata
    27541 tgaattataa atgcaataag cattgtagaa cctagactat cagctacatt tgatagcggt
    27601 gctcctaaat aagtatttaa aatatctgtt aagaaacttc cattagaaag catttttata
    27661 aatattccta ttacagtaaa tatcattata gttaacattc ctggtattaa ctttgcaaat
    27721 gatcttgaaa ctgctggtgg aactccatct ggcattttta tagttaattt ctttacctta
    27781 gataatctta caaatatctc agttgataat aatcctatta atatagaaga gaataaagct
    27841 gctgtaccta agtaggtttg ttgtatcatt ccccatcctt caactactgt agttcctccc
    27901 tcaggaacta ttttaccaat ttgtggagcc attataaaaa agatagataa agctataagc
    27961 ccagattgta acccatcact ctcataagat tttgctagaa aataagcaac acctattact
    28021 aagaatactg ccattgtacc tattgctccc caccaaaggt cgcctccaaa tgtggtccaa
    28081 ttttctccaa atatacttgc catcaaattt ttgtatgctt ccaatggtaa gttatttatc
    28141 aatgttccca aggcacctac catagttata ggtatcatta ctataaatgc atctcttact
    28201 gctactaagt gcctttgagc tcctatttta gcagctacag gcactatata tttatccata
    28261 aatgacatga atttttccat tttacatcct ccagtacaac tttattttga actatttaac
    28321 tctacagctc tatctaacac agcttttcca ttacatcttc cataatccat catatttata
    28381 acttctactg gaatattata tggttttgcc atttcagttg cttttcctaa tacatatcta
    28441 acttgaggcc ctaaaagtaa gacatcacaa ttttctattt cattttttaa gttcacttct
    28501 gatatagccc atatttttgc ttctataccg ttttcctttg ctgcatcctc catttttgta
    28561 actaataaac tagttgacat tcctgctgaa caagctaaca ttatttttat cattttttcc
    28621 cctccaaatt agattcatta aatatttttt aagtcataaa ttttttaaac tatacattta
    28681 aaatatgctt ttctattaat tttttcttaa tgtatatatc ttatttttaa atgtatatac
    28741 atttaattct tgatattatt atataccaaa tgtatataca tttcaagaaa actttttcat
    28801 tttatttaaa ttttattcga caacagcatt caaagttaat gtatatccat agtgatatgt
    28861 gtgtgagtac tcaaaaatct cactattttg taacttacaa atttggctta tatataaaag
    28921 tggctctggt tcttctaatt ttagattatc catacattct tcattcgata ttacagcttg
    28981 tacttctctc tctgacatgg ctatcttgta tccacacact tcttcaacaa aattataaag
    29041 agaattttca caatgagtct tatttaaatt tggaaataac ttaactggca tataagtaat
    29101 catatatgaa actcttgcat cattggctaa tcttactctt tttatttccc aaacattttc
    29161 ttctaaactt atattcaact tacttgataa attttcatct gcctgaacta tttcaagaga
    29221 taccaatata ttagatactt tatagccttt actttccatc tctttagtaa atccacttac
    29281 agacgatata ttatcatata tattatttgc aataactata ctacctacac ctctttgtct
    29341 agttatatag ccttctttaa ctagattatt aagtgcagac cttacagtca ttctagttac
    29401 attaaattct tcactcagct gtttttcaga agggattaaa tccccttctt tcaaactgtc
    29461 tgaatttatt aactctctaa catgattttc tataacttta taaattggct ctttcattta
    29521 attcactcct tattaattaa atcttttata taataagata attttatata caaataaaga
    29581 aaatgtctat acatttaaga taatttaaat tttattcaga catcttgtac aactttccga
    29641 gttttaataa agatataaat agacttatca ttaatatacc ttttaatata cttgataagg
    29701 ttatactcca ccatacacca tttagaccca acatttctgg tctagatata aagtaagcca
    29761 atggtactct tgcaccagtc agtatagtaa ttataataga aggtatatag gttctgccta
    29821 atcctttgaa agctcctgtt gttattattt ctaaacacat aaaaaattga gaatagccta
    29881 atattttaag ataaattgct cctttttcta ttgcctcact ttcatctata aaaatagaaa
    29941 atatactctc tcctgcaaat actaatacca aagtagttat tatcccaagt acagaagata
    30001 cagttatagc tattttgcaa cctttattta ttctagaata cttcttggca ccataatttt
    30061 gtccaacaaa actgcctaat gcaactgata gcccttcagc cgtcatccag gaaattgctt
    30121 ctatttgtga gcctactttt tgtactgcaa cagccacagg cccccatgat gctactatta
    30181 ctcctaatag cattgagaat actgtaaaca taccactttg tattgctata ggtaatccca
    30241 atttatacag aactttataa tatttagtct ctatttcttt aaatagtctt aatttaaaat
    30301 attcttcttt acttttaact attatatata taaaacatgt agtaactact atctgagcaa
    30361 atacagttgc aatagcagct cctgcaacac caagttttgg agcaaatccc catccaaaaa
    30421 ttaatatagg atcaagtata atattagtta ttaatcctac tgtattaata cgaaaaggag
    30481 ttttactatt tccaagaccg ttgaaaattg cagtaaatac tggattaata aaataaaata
    30541 ccatacctag agctactatt atcaaatact gtctagacat ggttataact tctaagtctc
    30601 ctaatctaaa aaaacctatc aattgtttat taagtactat taaagaaatt gtatatatta
    30661 ttgctagtat aatatttatt tctattgctg acttcatata tgatttagta gtgcttatat
    30721 tatttgcacc aatactttgt gctactttta tctctccacc tacttttgaa atcataataa
    30781 atgccattgc aagccatgga aaaaaaccag ctgttccaac tgcagctact gctttacttc
    30841 cggctttacc aacccatatc atatctatca tgttataccc catctgtata aatgaagtcc
    30901 ccattattgg aagtgatagc ttaagtaatt tttctgtaat tcttccttct gttaaattaa
    30961 ttctgtcttt cattttccac ctcttttata ttaaatttat ttgcttatat agctagatta
    31021 atcattttat ttgattgagt tcactatcct tcaatttata ttatcatatc tcaagattgc
    31081 atctcaacca tacttatgtt taagataaag tattttacct atctctgata aaattaatac
    31141 ttatgaatat aaatatagtc atttcatcaa tacatattat aacccttaac ttaacttcaa
    31201 ttaagtatac atactgtaat caattagaat attttataat gtatcttcca ttaagaaata
    31261 acaaatataa gtgatttatc tataatttta tttatacata aaatagtata ctgcaataat
    31321 aaaaaaacta tatttgaata ctttaagcat gtttactaat tgtaatttcc acacaaattt
    31381 gataatatat gaatatatta tggtgaaatt tatagatata tattaattat ggggagattg
    31441 tgggaggttc ttttcacaag acatttaagc tatgaaaata aataaaaaat ttaatagagt
    31501 tgatatgcag gtttcaatac ttactgcaat tattgtaata atatcttcct catgtgtata
    31561 tttttttaat tatacattaa cttataagag catgatatac tctttaagtg agaggtcaaa
    31621 acatatctat gaatatgtag agagaaatat tgataaaaat acctttactg aaattaattc
    31681 taaagaagac caatcaaaaa agtcttataa aagttctaaa atcctattag aatctgtaaa
    31741 aaatactact ggagtcatgt atctatatac tgctaaaaaa acaaaagatg gatcactagt
    31801 atatgttgtt gatggtttaa attcatctag aagtgatttt agaaatgctg gtgatttgat
    31861 agaaaaagag atttggggag atcttaacaa agcattagat aatcatatat tgttacctaa
    31921 aaagattaaa tcaacatctt ggggatacat atttatatcg tattttccta ttcacaatga
    31981 tgttggtaag gttgttggag taataggaat tgaatttgaa gcaaaacatc aatacaatac
    32041 atttaaattt ataagtatag ttacaccaat aatagcatta ttaacttgtc taatatcagc
    32101 attgattgca gtgattctat ttaaaagaat atctaatcct acatatactg attttgctaa
    32161 tacagattat ttaacaggcc taaaaaatag aaat
//
LOCUS       DAEPLF010000024        31749 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEPLF010000024 DAEPLF010000000
VERSION     DAEPLF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31749
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.024"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(103..942)
                     /locus_tag="KR314_000990"
     CDS             complement(103..942)
                     /locus_tag="KR314_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBH0327388.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            complement(963..1496)
                     /locus_tag="KR314_000991"
     CDS             complement(963..1496)
                     /locus_tag="KR314_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBH0327389.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            complement(1508..2317)
                     /gene="aroE"
                     /locus_tag="KR314_000992"
     CDS             complement(1508..2317)
                     /gene="aroE"
                     /locus_tag="KR314_000992"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBH0327390.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRIFEILNIESAYKNFEIPKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            complement(2364..3560)
                     /gene="pheA"
                     /locus_tag="KR314_000993"
     CDS             complement(2364..3560)
                     /gene="pheA"
                     /locus_tag="KR314_000993"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBH0327391.1"
                     /translation="MEDLTRYRNEIDNIDKEIIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAIDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKTISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYELEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAENNINMTKIESRPMKNASWRYFFYIDFECSIYNSKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            complement(3563..4642)
                     /gene="aroC"
                     /locus_tag="KR314_000994"
     CDS             complement(3563..4642)
                     /gene="aroC"
                     /locus_tag="KR314_000994"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBH0327392.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDKIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQRDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            complement(4620..5933)
                     /gene="aroA"
                     /locus_tag="KR314_000995"
     CDS             complement(4620..5933)
                     /gene="aroA"
                     /locus_tag="KR314_000995"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBH0327393.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNSTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            complement(5933..6997)
                     /locus_tag="KR314_000996"
     CDS             complement(5933..6997)
                     /locus_tag="KR314_000996"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBH0327394.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            complement(7019..8032)
                     /gene="aroF"
                     /locus_tag="KR314_000997"
     CDS             complement(7019..8032)
                     /gene="aroF"
                     /locus_tag="KR314_000997"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBH0327395.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKIVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            complement(8162..8317)
                     /locus_tag="KR314_000998"
     CDS             complement(8162..8317)
                     /locus_tag="KR314_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327396.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            complement(8494..9450)
                     /locus_tag="KR314_000999"
     CDS             complement(8494..9450)
                     /locus_tag="KR314_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBH0327397.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFIGIIIFKIKKLL"
     gene            complement(9789..10487)
                     /locus_tag="KR314_001000"
     CDS             complement(9789..10487)
                     /locus_tag="KR314_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0327398.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            complement(10521..13223)
                     /locus_tag="KR314_001001"
     CDS             complement(10521..13223)
                     /locus_tag="KR314_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBH0327399.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEVITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSSKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            complement(13401..15218)
                     /gene="ftsH"
                     /locus_tag="KR314_001002"
     CDS             complement(13401..15218)
                     /gene="ftsH"
                     /locus_tag="KR314_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBH0327400.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            complement(15249..15692)
                     /locus_tag="KR314_001003"
     CDS             complement(15249..15692)
                     /locus_tag="KR314_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0327401.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            complement(16183..17073)
                     /gene="metA"
                     /locus_tag="KR314_001004"
     CDS             complement(16183..17073)
                     /gene="metA"
                     /locus_tag="KR314_001004"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBH0327402.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(17103..18368)
                     /locus_tag="KR314_001005"
     CDS             complement(17103..18368)
                     /locus_tag="KR314_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBH0327403.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIIDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(18478..18580)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(18634..21291)
                     /locus_tag="KR314_001006"
     CDS             complement(18634..21291)
                     /locus_tag="KR314_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBH0327404.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNFSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKRKK"
     gene            complement(21419..22162)
                     /gene="yaaA"
                     /locus_tag="KR314_001007"
     CDS             complement(21419..22162)
                     /gene="yaaA"
                     /locus_tag="KR314_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBH0327405.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIIN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            complement(22172..22636)
                     /gene="bcp"
                     /locus_tag="KR314_001008"
     CDS             complement(22172..22636)
                     /gene="bcp"
                     /locus_tag="KR314_001008"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBH0327406.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            complement(22988..24685)
                     /gene="ade"
                     /locus_tag="KR314_001009"
     CDS             complement(22988..24685)
                     /gene="ade"
                     /locus_tag="KR314_001009"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBH0327407.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIASLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            complement(24991..26145)
                     /locus_tag="KR314_001010"
     CDS             complement(24991..26145)
                     /locus_tag="KR314_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBH0327408.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKFYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            complement(26390..27229)
                     /locus_tag="KR314_001011"
     CDS             complement(26390..27229)
                     /locus_tag="KR314_001011"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBH0327409.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFNNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            complement(27242..27838)
                     /locus_tag="KR314_001012"
     CDS             complement(27242..27838)
                     /locus_tag="KR314_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBH0327410.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            complement(27889..28539)
                     /locus_tag="KR314_001013"
     CDS             complement(27889..28539)
                     /locus_tag="KR314_001013"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBH0327411.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGVDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            complement(28585..29838)
                     /locus_tag="KR314_001014"
     CDS             complement(28585..29838)
                     /locus_tag="KR314_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0327412.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEKENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLREIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            complement(29862..30503)
                     /locus_tag="KR314_001015"
     CDS             complement(29862..30503)
                     /locus_tag="KR314_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBH0327413.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            complement(30705..31118)
                     /locus_tag="KR314_001016"
     CDS             complement(30705..31118)
                     /locus_tag="KR314_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBH0327414.1"
                     /translation="MIELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNLYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            complement(31269..31610)
                     /locus_tag="KR314_001017"
     CDS             complement(31269..31610)
                     /locus_tag="KR314_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBH0327415.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
BASE COUNT         9963 a         5659 c         3311 g        12816 t
ORIGIN      
        1 tcaatagcat atttaattat attgaatcca tatttattaa tacaattaaa tacactatcg
       61 cttacattta tacccatcta tatatagctt aaatattaat atttattttt ctaaagcttc
      121 tcttcttatt gatgattttt taaaatactc tatcaattta tttttatcat tgttaaatat
      181 agcttctttt actaaattta cttctgattc aaagttttct ataactttta gtaaattatc
      241 tctatttcct aaaaaaagtt cactccataa gtcttcattc atatttgcaa ttctagttaa
      301 atctctataa ctatctccaa taaatttacc tgtatctcta ccttcttcat cactatttat
      361 aagagcaact gccataacat gaggtaactg agaagtaaaa gctataatct catcatgttt
      421 ttgtgaagtt aactttttta ctcttttaaa tccaatctct aaaattaaat tttcaactaa
      481 ctctaaattt tttatgttgt ttctgccagt tggagtaatt atataatttg cgccattaaa
      541 aacttgttca cttgcaaaat caataccctt tttttcccta cctgccattg gatgtccaaa
      601 tataaaatct atatcatctg gaataatttg caacacatca ttgataagtg tttcttttat
      661 tcctgtagta tcagtaatta tagtcccttt tttaaaaaag tttttattat tatttagaaa
      721 atctacaact aatctaggat atatagatag tataattaaa tcagcttttt taaataactc
      781 tttacctgta gtacatcctt tttttatgat ttttgctttt tctgcttttt ttaaagtttc
      841 taaatctaca tctactccaa atacattttc atatccagct ttttttaatg ctttagcaaa
      901 agagcctcct ataactccta gtcctacaat aacaatattc attattttta tttcccccta
      961 gattattttt ctttaaagtc aaatttaaaa ttttcactga ctgcatttgt aatattatat
     1021 acagcattat ttaatgattt atcatttaaa atctctatat ctgaaaattt tttataaaga
     1081 tttattctct cattataaag attatacaat ctatcttttc cgttttttaa taatggtctt
     1141 gaattaatat ttatatcttc caaaatcttt tgtataggtc tatcaataaa aattattata
     1201 cctgtttctt ttagtatttc catattaaca tcctttttta ttacacctcc acctgtagat
     1261 atcactgttt tatcacttat agaaagttct ctacatgctt gagtctcata gttcctaaag
     1321 atatcttctc cttctgaaaa caactctgct atactttttt gagatatttg aattatataa
     1381 tcatccatat cacagaaact acaattatat tctttggcca aaagtttacc aattgtagtt
     1441 tttccactac ctggcatacc tattaatatc aatttttctt ttgttttatt tatcatgaca
     1501 atctccctta caaaaattct aattttagtt cattatatat aacatctaaa atactattat
     1561 ctattttagt atcttgccaa atttcctgag attttattgc ttgccctacc agcatatata
     1621 atccatcaca agttttttta cccatactat taccaattct taaaaactct gtttctccag
     1681 gattgtaaat taaatctatt aatatatcaa aattttgtat aatagatttt gacactggtg
     1741 aaattcctac attagggtac atacctactg gtgtagcatt taatattata tctcctttta
     1801 tatgtttaag ttcttcataa tctattaata tagctttact atttaataat tttttatcat
     1861 ccttttttct agttgaaaca taaagctttt ctataccaga atcaataaaa tatgtaagag
     1921 cagctttaga agcacctcca gttcctaata taactgctac ttttccttga acatcaatat
     1981 tagccatttt aaacatacta tcaagaccaa agtaatctgt attgtaacca tataacatat
     2041 tttctctgag taaaattgta ttaactgctc ctatacgttt tgcctctggt gatataaaat
     2101 caagatattt cataatacgc tccttatatg gtacagtaac atttactcct tgtattccta
     2161 ataattttat agcaccgtct agcttagata tatcttcttt tggaatttca aagtttttat
     2221 aagcactttc aatattcaat atctcgaata ttctcttgtg gatttgaggt gaaacactgt
     2281 gagagagttt ttcacctact agaccgaaaa aattcatact tacacctcct aataattata
     2341 aattaaataa aaatttatat tatctattca gttttatttt tatatactcc cataaattta
     2401 aaatatgctg tattatgttc tatcaattct aataagtcgt acactttaga attgtatata
     2461 gaacactcaa aatctatata aaagaaatat ctccatgagg catttttcat aggtcttgac
     2521 tctatttttg tcatatttat attattttca gcaaagtatc caagtacttt ataaagtttt
     2581 cctgcttcat gttcaacaga aaatactaca ctcattttgt ttgattcttc ttcaatgtgt
     2641 atttgattac tgattactac aaatcttgta tagttgtttg caatatcatt tatacaagga
     2701 cttattattt ctaattcata aatacttgca gcttttttgc ttgcaattgc tgcttttgtt
     2761 ttatcttgaa gttttcttac aagttctgca cttgttgctg tgttattaaa tggtatcaat
     2821 ttccatgtac tattttgttt taaaaattca ctacattgtc ctataccttg tggatgagaa
     2881 tatatttctt ttatatcttc aagttttgtg tcttttatcc ctattaaatt ttggtctatt
     2941 tttatacatt cttctccaac tatataaaaa ccgtactttc tcaaaaggtc ataagtttca
     3001 gagatagctc ctgtagatga attttctatg gggattattc catagtttat ttctttattt
     3061 ttaagtgcta tgaaaacatc ttcaaattct ttaaattgtt tttttgatat tgttttgttg
     3121 aagaatttat ttagtgcttc ttctgtaaat gaacctgatt ttcctggaaa tcctactagg
     3181 atattttttt tatttaattc atcttttaaa gacttatatt tatttacatt tgacaaatca
     3241 aatttcttat ttgatgaatc taaactaaac tcgattttct gctcaccatc attatctatc
     3301 aatctttttt gtaatcctct actaatttcc attatagaat taaaaaactg taccgtttct
     3361 tgtgagtatg ctttattatc taaattatct atggctctac ttagtacttt ttcttctcta
     3421 tctttttgaa gtatagtagt gttatttttc attttatgtc tggcaatttc aagaactgta
     3481 ttcattctct tttcaaataa ttgaataatc tctttatcta tattgtcaat ctcatttcta
     3541 tatctcgtta aatcctccat tattatcgtc ctttcatcaa atcaaaaatt ccaatggctg
     3601 ttactgcttc tattacaggt atagcccttt gaactataca agggtcatgt ctgcctttaa
     3661 tatataaaat atcgtccttt ttatctttta tattaactgt gttttgttgt ctgctaattg
     3721 aaggagttgg cttaattgcc actttgaaaa ttattggcat tccagtagta atccctccta
     3781 taatccctcc attgttattt gttttgctct taacttgatt tccttcataa tacatttcat
     3841 cattactttg agagccatac atatctgcaa gttcaaaacc taaaccaaac tctacacctt
     3901 ttacggcagg aacagaaaac aataaatgtg ataaagtaga ttcaacagag tcaaaaaatg
     3961 gatttccaag ccctacatta atacctacta ctgcacactc aattatacca ccaactgaat
     4021 ctctttgatt ttttgcatct ataatcgtat ttttcatggc ttcttccttt gataaatcaa
     4081 gtaaaggtag ttccaaagtt tgcaaatttg ataactgttg ttttgaaata tttacataat
     4141 caaaactcat atcttctata tttttaattt tttttatgtg tgctcctatc tctattcctt
     4201 tttgtgataa aatttgttta catatagccc cacaaaatac taatggagct gttatcctac
     4261 ctgaaaaatg acctccccct ctatagtcat taaatccaga atatctcaca ttacctgtga
     4321 aatctgcatg tcctggtctc attaaatttt taagctctcc ataatctttt gaacgagtat
     4381 cactatttct tatgattgca cataatggcg ttcctgtagt tcttccatta aaatatccac
     4441 ttagaatttc tggtatatca ctttcatttc ttgaagtaga tatagaattt tttccaggtg
     4501 ctcttctctt catttcttta tcaatcttat caaggtcaag ttcaatcccc gaaggaagac
     4561 catctatatt tatacctata gcatttccat gagattctcc aaaaattgat acttttaaat
     4621 tatttcccca tattccactc atcaattcga cctcctaaca tcttaaaatc tttaaaaaaa
     4681 tgtggataag attttgatac acactcaaaa tcttttaata ttattggttt atcacatcta
     4741 caagaagcaa ttgcaagtgt catagcaatt ctatggtctt tgtgactcca cacctctact
     4801 ccaccattta acttgcttac tccttcgatt atcaaactat cctctttctc ctctatattt
     4861 gctccaagtt tacttaactc aacatttatt gcatgaagtc tatcacattc tttaattcta
     4921 agcctaccag cattaattat tgtagtcctc cctatactta aggatgctac tacagataat
     4981 acaggtatta tatctgggca ttgagatgca tctatcaaag tactatttaa accatttgtt
     5041 attcctttta ttttgttgcc ttctcttaat atttccattc ccatcctaga taaaatttca
     5101 actacttcac tatccccttg aagagaatct tctttaaggt ctaatatgct aatatcttct
     5161 cctatagcat ctgctgataa ataaaatgct ccttgagaat aatctccttc tactttataa
     5221 tctctcgctt tatacgtttg atttcctttt ataataaatt ctttatagtt attgtttata
     5281 atttctaccc caaaatcttt tatcgttgaa agagttaaat ctaaatatcc tttagattct
     5341 agctcagtag taattattat ctttgaatca ctctctagtg taggcaatat aaacaataga
     5401 cctgtgataa actgagaact tatatttcct tttactctaa attcatctgg tttaagctta
     5461 cctgatataa tcaaatctaa ctgattttct ttgtaactat attttatatt ctgtctgtca
     5521 aatatctcat aataagtatc aagtggtctt tttccaagat tacctctacc tataaacctt
     5581 tttacaccat caaatgctaa agatataggt actaaaaatc ttaatgtaga gcctgattca
     5641 ttacaatcta ttgtcaattt aggttgattc aactgattct ctctatttaa aataccttct
     5701 ttagaaaaaa ttcccgaaat ttctaaaata tcttctttct tttctataat agctcctaat
     5761 gatgtcattg ctcttatagt agctattata tcatcagaaa aatctatatt agaaattcta
     5821 ctttttccat tgcttagaga tgaacaaata actgccctat gtgccatact ttttgatggt
     5881 ggtattttta catctccaga taatttactt ggatatattt ttagacttcc cattatctta
     5941 cctcctgaaa aaatttagaa gtagtattat atataatact ctctccaata tcttttaaaa
     6001 gtattatttt taatgtatca cctagatttt ttttatcaag agatatagca tctactatag
     6061 atttattatc tcttaattca atctcataag gcaaattata attcattaaa attgatttta
     6121 taagttctga tgtacctttt tttgtaattc ctatcttttc actttctaaa gttatcttat
     6181 acattcctat ggcaacagct tctccatggg tataacccgt aaaattgtag tatttctcaa
     6241 tagcatgacc taatgtatgg ccaaagttta gaagcattct atctccaaag tctttttcat
     6301 cagcttcaac tacagttttt ttaataaagc aacatttatg tataatgtat tctatatgtt
     6361 gcattacttc ttctctattt tttaaactta aaagtttatt gaaaaaagtt ttgtctttta
     6421 tacatccata ttttataact tctgccattc catctctata gaatttttct ggaagtgata
     6481 ataatacatt tgggtcgata aacacagctt ttggttggta aaagcttccc actaaatttt
     6541 tgcctctctc aaggtcaact ccaacttttc cacctacaga actatcaact tgtgctaata
     6601 aagatgttgg aatttgaata aaacttatac ctcttaaaaa agttgctgct gcaaatccac
     6661 ctaaatcacc tattacccct cctcctaaag taataatcaa atcttttcta gttaacttaa
     6721 agtcaagtaa actatcgtat attttaggaa gagtattaaa tgattttgtt tcttcaccag
     6781 attttaatat taaagattta atttcaaatc cagattcact taaattattt tttactttat
     6841 ccaagtataa ttttgctata ttttcatcag ttacaataaa aatcttattt ccaaaatata
     6901 ttttttttat ttcttctcct atattcttta gtatattttt tttaataaat atattgtagc
     6961 tattttcttt taaatttact aatagttttt cttccacgtt taaccatcct tttatagatt
     7021 atatttctct accaactgct gatgcgatag tctttaattc acttatcaat tcatcatatt
     7081 catttggctt aatcgattgt tttccatcac aaagtgcatg tgctgggtca ttatgaactt
     7141 ctattattag accatctgct ccaactgcaa ttgctgcctt agataaagag tcaaccatcc
     7201 atgatttacc tgctgcatga cttgggtcaa ctataactgg taaatgacta agtttcttca
     7261 cagctaaaat tgcacttaaa tccaaagtat ttctagtata tgtttcaaaa gttcttatac
     7321 ctctttcaca aagaactaca ttttcatttc ctccagccat tatatactct gctgacatta
     7381 gccactcttc aattgtagca gataatcctc tttttaaaag tattgtttta tttgttttac
     7441 ctaattcttt taataaatca aagttctgca tgtttctagc tccaacttga attacatcta
     7501 cattttcttc aaatatatct atatcttgtg ttgacattat ttctgtaact ataggaagcc
     7561 ctgttttttc ttttgctttt tttaataaat ccaatccatc atatttcaat ccttggaaag
     7621 cgtatggtga tgttcttggt ttgaatgctc cacctctcaa aaatcctgca cctgattttt
     7681 tcacatcttt agctatagat actatttggt cttctgtttc aacagaacaa ggcccagcta
     7741 tcatagcaat tttctttgaa ccaatttcca ttccattaac atttatgatt gaaggttctg
     7801 gatgaaattt tctattggcc tttttaaaag gctctactac atgcataact ttttcaacta
     7861 tcttatttgc ttctattctt ttggcatcta ttttactagt atctccaact aaacctaaaa
     7921 cagttaattc tgttccatct atgggattaa cttctactcc ttctctccca atagcctcaa
     7981 ttaatttttt aacttcattt ttatctgctc ccatttttaa tactactatc atttttacta
     8041 cctcccaata ttataatttt atatttaaaa taaaaaagag aactcactaa tgagttctct
     8101 aaaataatct atcaagcact aaatcttata taaatatata aaataatcat ttttataaaa
     8161 tttacttatg ctcaaaatca ctaaaataag gcctcaccag aaaggaagta catatatttt
     8221 tgtaaaagaa atagccagcg ctaaaataaa agccccagaa cataaagtaa aaaaagctat
     8281 aaagttttgc tgttagttct tttaatattc ctttcatagt attatcctcc atttttataa
     8341 tttattattc ttcttgttta ataattataa caaagaaaat ataattgtca atattttcta
     8401 taaatatttt caatttaaaa tattttttta tttacttaaa tcagcttctc aattgatgta
     8461 tttttataaa atcaattaaa catcatctgt aatttacaat aattttttta ttttaaatat
     8521 tataattcca ataaaaatta tacatagcat aaaaaataaa gttgctggat atccataaat
     8581 ccagtttaat tctggcatat atctaaagtt cattccatat ataccagtta taattgtaag
     8641 tggtgctgat atggctgtta acaaggttaa ttttgtaatt aagttatttg ttttctcacc
     8701 tactctggaa gaataaatat ctaataattt atctgctaac tctcgtgttg acaatgcgaa
     8761 attgtataat ttgtcaattc caaaatctat accttgaaaa ttttctaaat tatatttttt
     8821 tacatttgaa tattttaaat atcttatatt ttccttaact attctatccc caatatacaa
     8881 aagaggtcta gtattcttaa ctataattct tgttaatcct cttacatcac ttattctaga
     8941 tacatgattc ttattaatgt tgtccatcat ttcatcttct atttgaagaa ttaactcttc
     9001 aactttttcc aaagactcaa aaccattttt tataacttct ctaagaatca agtagtttat
     9061 cttaaataga ttaataactg gatttttttc aagttgagag ttcttcaaaa taatattttt
     9121 tacaaattca aataaaaagt gttcttcatt cactacaacc aatataaaat tatctgataa
     9181 atacatattt acttcttcta taacagcttc tccatctctt agttcaaatg tatttaggct
     9241 taaaaaatcg taattatcaa ataaatcaag tttggttatt tcatcaaatt taagacaatc
     9301 attaaaagtt atttcatcta tatccaaagt ttccttaagc aattttaaat tttttggagc
     9361 acttaatata agataagaat tttctgctac ataaaattta tctctaaaat cttcaatagt
     9421 ctctcttgta tttaaattca gtatatacat acatctactc ctaaatttca atttacattc
     9481 taaaaatttt ttataatcct tactgtaatt attttacact attttaacaa ttattattta
     9541 atatttacat taaatttatt ataaggtaag tgttaaatac atttattatt taaccaaagt
     9601 ttttattaca gattacacta tatacttatt ttaaaacata tttcatatta ttgatactaa
     9661 aaagctaaaa aataaaatgc atgtgctaaa ctgacaacaa gcacatacac ttcattctta
     9721 ttttaactaa actatataat tttaatatat attaataagc acataatcta ataattaata
     9781 tattacaact attcatcatt taatctgtat cctacaccaa cttctgtaat tatatatctt
     9841 gggtttgcag gttgtttttc tatttttcgt cttaatgttg ccataaagac ccttaaagac
     9901 ttagtctcat tccctataac actaccccat atttctttta ttataaaatt atgagtaagc
     9961 acttttccat gatgctttgc aagtaatgaa agaatattat attcaatagg tgtcaaatgg
    10021 acttcaacat catcaacaat aacttttctt ttatcaaaat caatcaataa tttatctact
    10081 tcaaacgtag atttaacatc atctttttgt tgagcttcta cttgtgaatg tctaaatgcg
    10141 actcttactc ttgctaataa ctctactatg gagaatggtt ttgttaagta atcatctgcc
    10201 cctgcatcaa atacttctac cttatctcta tcttgctctc tagctgaaac tacaattatt
    10261 ggtatatttg aaaattgtct gatagctttt attacctcta ttccatccat atcttctaag
    10321 cctaaatcta aaagaacaac atctggagaa tatgagacag caatagatat accttctttt
    10381 ccagttatag cctccttgac atcataatcc tgtgttaaaa gtgcagtaga tataaatttt
    10441 cttattgtct tatcatcctc tactaacaat ataagttttt tactcatttc ttcctccaaa
    10501 actaatttta aaatttaaaa ctattcattt tccttaggca aactaaactt aaaagttgaa
    10561 cccttatctt tattgttttc taccataatt tcacctttat gagcttgaat tatagattta
    10621 caaattgata atccaagtcc aacacccttt ctggaatctt tagctccttc ttctccagta
    10681 aaaaatctgt caaagatatg ctttttaagt tcctttgata taccaggacc attatcagat
    10741 acttcaaacc aaacataatc atctttctcg tatacattta tctctatctt acaatcttcc
    10801 tttgaatatt tcaaagaatt atctattaaa ttaattaaaa cttgttctat taattttgca
    10861 tccataggaa caaataatat ctgttctggt atattaatat ctataagtgc attttcaatt
    10921 ctttttttta ttttttgtaa cgcttctgat ataatttcct caaccagttc agaatgtttt
    10981 ttaacttcca atttcccttc atctatcttg gtcatactaa gtaaattttc aacaagtctt
    11041 attaaccact gagtatcttc atatacacca ctaagtaatt catctattat atcttgacct
    11101 atagtaccct tgttttttat aattgtactt actgcaccag atattcctgc tagtggagtt
    11161 ctaaggtcat gcgaaactgc tcttaaaaga ttacttctta atctttccct ttcaatctca
    11221 agattggtat tttctttagc ttctgacaag atttctctat caagcgctat tgccatttga
    11281 gcaattattg tttcaattaa gcttatatct tctgtatcca attttgaatc aatacatgat
    11341 attcctacta ctcctaaagt taaattcatt cctacaattg gtatatagta tccctttgaa
    11401 ccaggaagtg tatccgtgtc atttcctgcc actgttgaat tggataaaac ccaacttgca
    11461 acagcttttt catcttcact atccaaatca tttttatcct tattattagt attttttttg
    11521 taaagttttg atttattatg cttatctaca tttaaataac aaattacatc tctacttaag
    11581 ctaagagaca aaagttctat acctttgttt actatatcat ctttacttga taatcttaaa
    11641 aatcccttaa ttatttgata tatcattttt gtattttctt ctcttttagc agcagtttct
    11701 gcttcatgct gtatcttaga cgtaagagtg ctagttatta tacctactat tgagaataca
    11761 accagtgtta ctatatagtt tgaatctgct atagataatg tatataatgg tgctgtaaaa
    11821 aaataattta aaagaactat attaaataca gaacttatta tacctgtact atacccttta
    11881 gtccacatag aaactaaaac tacacccaac atatatatta acaatatatt ttctcttata
    11941 aatcccatgc tttgtactgc atatgctaaa atacttatca tcaaagtaat aaatataagt
    12001 tttataaaat caactttgct aattttaaat ttagatttta ttgatgattt atccttttta
    12061 ggtttgtatt taacatctga ggcaaattta tatggaatta tatgtatatc tatataatca
    12121 acttcatcaa ttaatttgtc aactatatct tttttaaatc ttttactaaa tcttttatta
    12181 cttgaatgat ttctacctat tacaattttt gttacatttc tcagttttgc aattctaagt
    12241 atttgctcta ttatattttc accatgtaaa acaattattt ctccacctaa tttcttagct
    12301 aattttaggt tattttgtag ttgtttttgt tcatcaaatg gaagtcttgc tgtgtttgga
    12361 gtttcaacat ataaagctat ccattttgca aatgtactgt ctgatattct ggatgcagtt
    12421 ctaataactt ttgctgaaga tggagatgga cttatacatg caagaagtgt atctgatgta
    12481 ggtattacag ttacatcacc ctttgatagt ctggtcattt gaacttcttt attaactctc
    12541 tctgctgttt ttctaagtgc gatttctcta agtgcaacca ggttatcttt tataaaaaaa
    12601 ttattttttg ctctagtagc ttgttctttc ttatatatct taccttcatt gaacctttca
    12661 agaagtacat caggttcaac atctataagt tctaactgtg tatttgtatc caaaaactta
    12721 tctggtatag tctctttaac tgatacgttt gtaattacct caacaatgtc atttagactt
    12781 tctaaatgtt gtacatttac agtagtgtag acatttatgc ctgctaacag caattcttct
    12841 atatcttgcc atctttttgt atgccttaac ccagcaacat tagaatgggc aaattcatct
    12901 actaatataa cttctggttt tcttgctaaa gcagcatcta aatcaaattc ttttagtttt
    12961 atagatttat attctatctc ctttacagga agaatttcca agccatcaag tagagccaat
    13021 gtttctggtc tagtgtgtgg ttcaatatat ccaactacta catctacacc cacttttttt
    13081 aaattttgtg cagattccaa catagagtag gtttttccta ctccagctgc ataaccaaag
    13141 aatattttta attgacctct acttagtttt tcttcttcat ttttaatttt ttctaataga
    13201 atatcaggat taggtctatc cattaaaaaa cctccttaca ccattcatta ctattataat
    13261 atcaattccg atataaaatt gtagttaaga tgtattttta atgattaaga ttatgtaaag
    13321 atttaaatat gaatttaaaa aatttagttg taaaataaaa atgcctaaca ataggcattt
    13381 ttatcttgtg tataatcatt ttagtttaag ttaagtattt ccataaactc atcacctgta
    13441 atagtttctt tttctaataa ataattagca agttcatgca atttatcaat attattttct
    13501 aagatttcaa ttgcattttt gtgacaagcc tttactattt taaatacctc ttcatctatt
    13561 ttagcagcag tgtcggaaga acaagcaaga gaagtatctc ctcctaaata ttgattgctt
    13621 acagtttcaa gagcgaccat atcaaactct tcactcatac caaggcgagt aaccatagct
    13681 cttgcaattt tagtagcttg ttctatatca tttgatgctc ctgatgtaca agtgttaaag
    13741 ataacctctt cagcagcacg accacctgta aaagtagtta ttttatcaat tgcttcttct
    13801 ttagacataa gtacactctc atcttcagca acttgcatag tatatcccaa tgctcctgaa
    13861 gttctaggta ttatagtaat cttatggaca ggtgcagaat gtttttgcat agcagcaaca
    13921 agtgcatgac ctatttcatg atatgctata attttctttt ccttatctga aattacagct
    13981 cctttacgtt gatacccagc aattacagtt tcaacagatt catcaaaatc ctgttgttct
    14041 actatatctc tgccagactt aactgctaaa agtgctgctt cgtttacaat atttgcaagt
    14101 tctgcacctg atgctcctga tgtagacctt gcaattgcat tataatctat atctgagctt
    14161 acatttacat tttgagcatg tacctttaat atagcttcac gtcctccaag gtctggaagt
    14221 tcaactggta tacgtctatc aaatcttcct ggacgaagta atgctttgtc aagactgtca
    14281 ggtctatttg tagcagcaag tattactact ccaattccac catcaaaacc atccatctca
    14341 cttaacaact gatttaaagt ttgttcacgt tcatcatttc cacctaatcc acttccatta
    14401 tcacgctttt taccaatagt atcaatctca tctataaaca ctatacatgg agctttttct
    14461 tttgcttgtt taaataaatc acgaatccta gaagctccca ttcctacaaa catctcaaca
    14521 aactcagaac cagatattga gaaaaatggt acatttgctt cacctgctac agcttttgca
    14581 aggagtgttt ttcctgtacc tggaggtcct actaaaagtg ctcctttagg catttgtgct
    14641 cctatctttt tatacttttc tggattgtgt aagaaatcta ctatttcagt taaagcttct
    14701 tttgcctcat cttgaccagc tacatcatta aatctttttc cactttgagc agatacatac
    14761 attttggcgt tgctttttcc aaattgcata gcattaccac ccattcgctt ttgcatagac
    14821 ttcatcatta agttaccaaa tagcataaga atacctatag gtattatcca tgtaagtaaa
    14881 atacttaaaa gaggtgaatt ttccttagga ataaccttag taaattctac ttttgcattt
    14941 tttagtctat ctaccaattc tgggtcatcc attctaccag ttatgtatat attcttatcc
    15001 ttgctactag atggatttat tgctattcta ttattttcta tctcaacaga tttaaccttt
    15061 cctgtatcca acattgtgag aaactttcca tagtcaatct caaccatttt tttatccatt
    15121 aaacttggaa atattaaagc atttaaaaac attactacta ttattataac tgtataataa
    15181 tagagtgctg accttttttg gggtttatgt tcatccataa ttttattctc ctttaaatta
    15241 tttttatgtt atttgcaatc aatgtaagtt aaaagtttat ttaatttctc taaaccttct
    15301 attatatctt ccatatcttg caatttttca ctggtcatat tttcaaaata actatcaaaa
    15361 atactacttg aaatatgttc ttgtatatcc aatgatttat ttgtaacttt tacttttaca
    15421 attcgttggt cttctatatc tcttatgcga tttaaaaagc catttttttc aagcctttga
    15481 cagattactg atacattact atttgtcatg tttaagttct tccctaaatc gcttattttt
    15541 ttctctccat gttcacataa ctcagataaa atcaaagctt gcactgctgt aataccaagt
    15601 ggctgataaa aatcacttaa taaagttgta aatttcatat ttatatctct agtcaactct
    15661 agtagctctt tcttaaaact catctcatcc atacaaacgc tccctacgaa aatactttat
    15721 ttcaaattta ggctgaagtg catactgtaa ataaatactt aatactataa tgtttaatgt
    15781 attaaatatt atagtattaa gtatttattg tcaattacaa tattgtaact tcaattggaa
    15841 ttttgttatc ttattgatat ttaattttat acaggatatt tttaataaaa tttaataaaa
    15901 aatcgatata tatattttac actgatttct ccctttttaa tctcttttaa aagttaaaat
    15961 acaaatttat tgatactata aaggtttagt ctcccattat cgcaccatat ataaaattaa
    16021 aaccacctct atctacatac acctaaagtg attttcttaa aatataaaac tactattata
    16081 aggttaaatt taaaaaaaat aaggtataaa gtatcattaa gattaacttt atacctataa
    16141 atttctatat ttttttagtt aacatttata tactcttaaa atttattgta tcaaatagtt
    16201 ttttatccaa tttctaaaca acaattctga atgttttttc cattttacag ttggctcttc
    16261 tgaagggtca tcattaatat agtaattttg aggtatagat attttatctc ctttatttat
    16321 atccctctta tattctttat caagagtgta tttgtcatac tcgctatgac ccattacgaa
    16381 cacatctctt gaatttgtaa ttatataggg accagcttct ttagaatgac tcacaatgct
    16441 caactcttta ttctctttca aagcttcaat atttacagta gtatgtcttg aatgtggagc
    16501 aaaaaattca ttttcaaatc catctactat tttactatct ttatctacct tatgcttaaa
    16561 tactccaaaa catttttgac ttagtggaag tttttttaca ttgtagtatt tgtaaagact
    16621 tgcttgagct gcccaacata tatataatgt agatttagtt tttctcttag aataatccat
    16681 aatttcttct agttcattcc aataatcaac ttcttcaaac tccatttgtt caactggagc
    16741 tcctgttatt ataaagccat caaaatactc tcctttggct tcatcaaatg ttttataaaa
    16801 ttctttaaca tattcattac aacttctttt actttctctt gttgaaattt taataaattc
    16861 aacagacacg ttgaatcctg ttttatctat ccttcttagt aaatctaatt ctgtatctat
    16921 ctttataggc attagattta gtatagctat tttagtgtct atgttttctt catactttaa
    16981 ttccttttta aaatcttctt tataaattac atctattcct tcatctaata atttattaat
    17041 aacaggtaat ccctttggta gtattaaagc catatctttt cacttctctt tctcttagaa
    17101 tattagaaat ttaaagcttg atttaaatca gctattaaat cctctacatt ttctattcca
    17161 acagataatc ttattagtga aggcaatact ccagatgcaa ttaattgttc ttctgttaat
    17221 tgtctatgag tagttgaagc tggatgtata acacaagttc ttacatctga aacatgtgta
    17281 accaaagatg ctatctgtaa tttttccaca aattttttgg cattttctaa cccacctctt
    17341 actccaaatg ataaaacacc actacatcct cttgataaat actttttggc attctcataa
    17401 tacttgttat cttcaagtcc tgggtaatta atccaatcta cattttcatg tttttctaaa
    17461 aatctagcta ttttaagtgc attttcacta tgtctctcca ttctaagatg tagtgtttca
    17521 acatttaaat tagtaagata cgcattgaat gggcttaaac aatttccata gtctctaagc
    17581 aactgaactc ttgctttagt tgcatatgcg gcatttccaa atttttgagt atagcttatt
    17641 ccatgatatg ttgggtctgg ctcaactaat tctggaaatt ttccattatc ccagttaaag
    17701 tttccaccat ccactataat tcctccaaca cttgaagcat gcccatctaa atattttgtg
    17761 gtagaatgag taactatatt tgctccatac tttaaagggt tacaaagcac tggagatgct
    17821 aatgaattat caataataaa tggaacatta attctcttag ctacatttgc tattttttca
    17881 aaatctatga cttcaagagt cggatttgca agtgtttctg caaatacaac cttagtattt
    17941 tcctttgcaa gttctactat ctcatcttca cttgaatcta agtcaaacga tattaaatct
    18001 ataccaaatt ttttaagact aggtcctagt aaattgaatg ttcctccata tacttttgaa
    18061 gaacaaagta tactatctcc tgatttacat atatttaaaa ctgccaacat atttgcagat
    18121 tgccctgatg atacagctac agaagatact ccaccctcta gtaaacttat tttttcttca
    18181 aaagcttgaa tagtaggatt gcttattctt gaatatatat gtccatcaac ttttaaatca
    18241 aataattcag caagttggtc tatactgcta tatttaaaag ttgtactttg atataaaggt
    18301 aatactcttg gttctccatt acctggttta taatttcctt gcacacatat tgtttcttta
    18361 ttatacattc ttgtatcccc cttagatttt agtaaaaaaa attaaactat ttatcagatg
    18421 agagttaaaa taaataaagc ctgagtatat aactcaggct ctgtataagc ttaattatac
    18481 tcatctctca gctttcgccg caggatttag caccaatgta tataaatata ctggttgccg
    18541 ggtttcatag ggccagtccc tccacctctc tggataagat ttatttaatt tttctatgtt
    18601 ttatatctta tatatttttc ctaattttgt caactatttt tttctttttt ttcttttaat
    18661 aattttccca acctctactt gaattacaga cattatggct aaaacaatcg ttataagcca
    18721 ctctaagtta tttaaaactg ttaagttaaa agcatttcta aaaacaggta caaacagtat
    18781 tactgctatt aaaaatgcag aagttaaaaa tgagattaaa agaaatggat ttttaccatt
    18841 atttctatta aacattgaat ctgtatttga acgctgacta aatgctcgta acatctgtga
    18901 aaatgctaat acacaaaatg ccattgtttg acctacagaa tgattttcta tattaagacc
    18961 aattttatat gctaaaatag tcattattgc cacaaatata ccttgggtaa caactctttg
    19021 tataagattt ttttcaaaca aagtggttga tttcactggc tggtgcttca ttatatttct
    19081 gcttggtggg tcaactccaa gagcaagggc tggaagcgta gcagttgcaa ggtttaccca
    19141 tagaatatga acagcaagaa gcggagtatc ccaatttaat aatattgcta caaacaacgt
    19201 aagtatctct gctatattac caacaagcaa aaattgtata actttttgta tgtttctata
    19261 tacacgtcta ccttctttta ttgcataagc aattgttgta aaactatcgt ccattaaaat
    19321 catgtcagaa gaatcttttg caacatctgt accacttata cccatggcta taccaatatc
    19381 agcagacttt aaggcaggag agtcatttac accatcccca gtcattgctg gtacttctcc
    19441 aattcttttt agagattgta taattctaag tttatcaaac ggggacacac gagcaaatac
    19501 tgtagtattt ttaacagctt catctagttc atcatcacta aaattatcaa gttcatcacc
    19561 agatatcaca gtattttctt cactccatat tcctaactca tgagcgattg ttgtggcagt
    19621 aactttatgg tcacctgtta tcattatggt tcttatacct gcttctttac aagtacttac
    19681 tgcatcaatt acctcagttc ttggtgggtc aatcatacca accataccta taaatgtcaa
    19741 atcatattct atattttcac tatcttcttt tggtatagat gaaatctcac gcttagcaaa
    19801 accaagtaca cgcaatgcat ctttagacat gttaagacat aattcgtgta tattcttttt
    19861 atccacttcg gtaatgtttc tttcaccttt tgaagttaat attttagtac ataggtcgag
    19921 aatttcgtct actgctcctt tagtataagc aataatcttt tcatctatat tatgaacagt
    19981 tgtcatacat tttctatctg aatcaaaagg ctgttcaaat acacgtggat attcttcttc
    20041 aagagcttct tgatctattc caaatttttt ggccataaaa attaatgcgc cctctgttgg
    20101 gtcacctaat atttcatctg ttttatctgg attaatacat gcatcattac aaagtgcacc
    20161 agcataaaca atctctttat acactaatgg atgtttttca gatgcagtat ctattgatgt
    20221 cacttttcca gattcaaaat ctccattgac agcaatatgt gtaacagtca ttttatttaa
    20281 ggttaatgta ccagttttat cacaacatat tactgtagct cctcctaatg tttcaacagc
    20341 aggtaatttt ctaattaaag catttctttt agccatacgc tgtacgccta aagccattac
    20401 aatagtagct gttgcaggta atccctctgg aataattgat atggcaaggg aaattgcaat
    20461 cataaacatt ggcaagagag gtctctgata aaatgcacct attgcaaata taacaacgca
    20521 tacaataata cctatgacac ttaatgtctt tccaacagta ttaagtttac gttttatagg
    20581 agtatcaaaa tcatcttgtg aatctaaaag atgtgctatg ttacccactt ctgtatccat
    20641 accagttgct actacaacac ctacacctct accatatgtg actattgaag atgtatacgc
    20701 catattgcta cggtctccta atacacattc tttaggtaag attgtatctg catctttttc
    20761 tgatggaaca gattctcctg ttaaagaagc ctcttgaatt tttaaacttg atgtttctat
    20821 taaacgaagg tctgctggaa ccatagctcc atcttctaaa aaaacaatat ctcctactac
    20881 aagattactt gctggaataa cattttcttc accatcacgc ataacacgag ctgtaggtga
    20941 gttcatattt tttaatgctt ctaatgatga ttctgctttt ttctcttgaa caattccaat
    21001 aactgcattt actgttacaa ttattagtat tacaaaagct tctgtaaatt cgccaaatat
    21061 caaagataat attgctgctc ctattagtat cataaccata gcatctgtaa tttgactcca
    21121 aagcatttgt aaagttgttc taggtggttt ttttctaagt tcatttaagc cattctcttt
    21181 aagccgtttt tccgcctcgg tatcggtgag cccctcctct gatgaatcta gactttctaa
    21241 aatttcatgc gttgctcttg aatgccaagg catctgtttg tgattttcca ttttggaaga
    21301 ccactccttt ctttttttaa tttaagaata attatatttt aaaatcaagt tcaattcaat
    21361 acacactaag tatattttac ctaaatttaa caatgccatt taatatttaa caaaacttct
    21421 actcttttac aaatatgtat tcactattgt tagataagtt ttctttatac ttataacctt
    21481 ctaaattaaa ctttttcagt tcttcaatat ctcttatcct attttttaca atgtattttg
    21541 tcatcatacc tctagctttc tttgcttgtg tagattccac tttcaatatg ccatttttat
    21601 ttactttaaa agtacatgta atatatgtat cactatcttt aataaatttt tctatggact
    21661 tgctgtattc atttgatgaa agattaatga tagctgtttt ttctttgatt agttctttat
    21721 atatacgatt tccccaatat tcgtataaat tcttaaactc acctactcgt aattttgtct
    21781 gcatttctaa tctatattca tatatactat catatggttt tacaacacca tacaatccac
    21841 ttatgattcg aatacagcta tttgcaaact cttcatcttt ccttgtaaaa ttttctgctt
    21901 ctatattttt atattgtatt ccatcataag ttaagattgc agggtttcct aatctatcaa
    21961 atttaatgct ttcaaatcta tttttattta atacagctaa tttttcatta attttcattt
    22021 tatttttaaa atcttctata ccaaatgtct ttatattttc cactatttct tttgtgttat
    22081 ctataaaaca aggcaaactc aaatctctat caaatacttc tatattcttc atatttttag
    22141 ctggtgataa cattataatc ataaattctt attactcctg tttttccaaa tattcaagaa
    22201 tctcttgtgc atttgtgtct ggttttgctt tttcaaagac tttttcaata attccatttt
    22261 catctataat atatgtcgca cgtacaactc ccaatgcagt ttttccatac atttttttct
    22321 ctttccatac atcaaaagct tttatagcaa ctaagtcagg gtcagaaagt aaaataaaag
    22381 gaagttcatg cttttctgca aatttttgat gtgattttat actatcttta cttatcccaa
    22441 taacttgtat atcttccttt ttaaatccat catatgcatt tctaaaagca caagcttgac
    22501 gtgtacatcc tggtgtatta tctttaggat aaaaatatac tactactttt ttgcctttaa
    22561 aatctgacat acttacctta tttccatctt tatcttctag tgtaaattct ggtgcttttg
    22621 ttcctatact caacataaat atctctcctt ttgtttttat taataatttt atactatgct
    22681 tatattatta ccctataaat ctatatataa ataaaatatt ttatttttta tgtataaagt
    22741 ttcataaata tataattttc atgtatttat attctaaaat agactgaaaa tagttttatt
    22801 aagatatcta tgcttaaaac aaggttaaat gatagattaa ttaaaataag gatatctaaa
    22861 aataaaattt ttagttcatt agatgctctt ttttgtatga aatcaaatat attttcatca
    22921 tttcaacaat aaaaagagac ttatccctat tttgagaaag cctcatttat tattactttt
    22981 atattatcta tataaattta aattcctcaa catcaaatag acctaaatct gtaagtctta
    23041 tttgtggaac aactggaagt gccataaaag aaagtgtaat aaaagggtct acatactctg
    23101 gaactcccat ttttcttgct atctccaaca tattatcagt aatttcttgt acttcttcag
    23161 ctgtaaaagt actaattaat ccagctactt gaagacttag gtgtgcaaga acttttccat
    23221 ttgatacaat tacataacca ccttgtattt cctttaagta atttatagct actagaatat
    23281 catcatcatt atcccctgca actataatat tatgagaatc atgggccaca gtagtagcaa
    23341 ttgctccatt ttttatgcca aatcctttta gtgaagcaat tgctacattt ccagtcattc
    23401 tatgtctctc aacaacacaa agttttgaat actctttatt tggaacaaaa tatccatctg
    23461 cacatggtaa agattcatat accttatttg tcaaaatttg ataaggtaca atctccatta
    23521 cataattttt ttcacttact tttaactgta ttttttcttt attaatatac ttaaacttga
    23581 ctgtattaag caattcttta tctttaatct cataattgta attagaaaac atctcttcgt
    23641 ttaccaaatt accatcttta tatacactat ccacttccat tttgtctaaa tcattcaata
    23701 caacaatatc tgccttataa cccgcaccta ttgctccaat tttccttaac ccatatgctt
    23761 ttgcagaatt ataagttgca acttttatag ccctaacagg ttccatacct aaatcaatcg
    23821 cacattttat attccatctt atgtgacctt gtttttctat atcatctaag tgcttatcat
    23881 ctgtgcaaaa cataaattct tcaattggca agttatgctt tagcatacca cttactatag
    23941 actttaaatt ttttgctgcg cttccttctc ttattaaaat ttttaatcct gctctcaatt
    24001 tttcatatac ttcatcaaaa gttatacatt catggtcatt ttctattcct gcacatacat
    24061 atgtctgaag tgatttacca tttatattag gtgcatgtcc atctacaact ttatttttaa
    24121 aaagctctag tttatccaag atttcatttt cagaatttat aacatcatta aaacacatta
    24181 cctctcctaa acctaaaatt ctaggattat ttacataact ctccatgtct tttgcactaa
    24241 atttaccaac tccattagtt tcaaaacttg ttgcaggtac ggatgaaggc accataatat
    24301 atacattaag tggtatatct tttgtcgatt ctaataaaaa atctattgca ctagctcctt
    24361 ttacattgac caactcatgt gggtcggcta tgatagttgt cgtacctgat tttaataatt
    24421 ttttcgataa ttcaattggc attaccattg tagattcaat atgcatatgt gcatcaataa
    24481 agccaggtga tatatattta ttgctacaat caatttctac attgcccttg taatcgccaa
    24541 tgccaacgat agtgtctcct actattgcta catctcctgt tattaactct tctgtaaata
    24601 catttatgat tgtcccattt tttagtacaa tgtcagcttt ttctcttcca cttgaaacat
    24661 cacactgtct ttttaaatta cccattttta caactcctcc taagtttttt atatcaatat
    24721 actggctgta ttacgtatta tagtttaatt tattaatata tattatatat gagttttgat
    24781 agaaagtatc acttgaaatt tgttccataa ttcctttaat taaatagaga gaacatgagg
    24841 aataatatta tctcccatgt tctcattaat atttaagcaa taatttaatt catattgaat
    24901 tttttatttt tatatttaat cttcactatt cttataaata aatttatgtg ctttattatt
    24961 tttttaatac ttctttattc gtaattatat ttaacctaaa aatgcataaa ctatcatacc
    25021 tacaagtatt gctggtatag tagtaataat catagcccat aaagaagatt ttttatccat
    25081 tgctaaaagt gggaaaagtg catctccatc ttgagaaata gagttggcta tcactgcacc
    25141 aagtggtata gttcccttca aataaaatga cataagcaca atttgtatcc cacaacctgg
    25201 aataagtcca agacctgcac ctataaatac acttattaca cctgttgata atactagttt
    25261 aactaagtat tcatctccac ctaaaattaa tattatcaaa tcgtaaataa gatatgcaat
    25321 aaaaatccat gtaattacaa atgatatttc tccaacagaa tggataagca tttctcgcaa
    25381 agaaatcttt ttattttctt cctctttttg attagaattt ttaaatacct ttttaaaaca
    25441 ccacatataa attattgaaa ataatattcc aatcactgct attacttctt ccaatgaatg
    25501 taacttctca attataggaa gattaagtcc agagtgagca agtgacataa atataaaacc
    25561 gattactata attcctatat aaaatttata tccctttcca tgtgttatta taaatgcaag
    25621 tctatttgta tttccatgag atttagctag agtgtctaaa attatttcat catgattatg
    25681 ttgttcttta ttttctttag ggttattttt acttatatca tatttatctg attttctacg
    25741 gttttttaac cctaattttt cttctagttt aaagtaatcc actaattgtc ccgttattat
    25801 tcctgtaatt gtactaacta tagtgacaaa aaaatacatt ttaatatttg atgaaataag
    25861 tacaaatgct gcatctccca tactagctat taaacttgca attattgctc caaagcttaa
    25921 ttttccattt atatacaatg gcattattgc cagacttccg ccacaacctg gtatagctcc
    25981 aattaaagca cctattaagg gttgaaattt tctgtttttt gatatgatat tagtaaaatt
    26041 tccactagtt ttataattaa tatatccaaa taacaaaata aaaaatccaa tcatagaacc
    26101 tacatgtaaa aacgcctctt cagaagcgga tatcaacatt tccattaatt tacctcctag
    26161 actaattttg caacaattat attatattct attttctatt gataatcaat atcaatattt
    26221 cttttgaatt tttaatatta aaaagtgcgc atcttatact cttagttttt catcttaaga
    26281 tacacacttt ttgatattag gttatctatt taccacatgt aatatgaaat caaaggtgga
    26341 tattatgata aattataaca atgtccacct atttttatta cctatcaaat tataaatttt
    26401 caaggaatgt tacaacttct tctattatcc aattaggtgt agatgcccct gctgtaatac
    26461 ccaaattttt atatttttta agcgtattta catccaattc atctttagtc tctattgcaa
    26521 aagttggtac taagtcttca caaaccttta ctaacttttg agtatttgaa ctgtgtttac
    26581 ctcctataac tatcatacaa tcaacttctt tagctaactc tttagctgat tcttgtcttg
    26641 tttttgtagc tgaacaaatt gtatcattaa atactatttc ttctaattta tctgatagtt
    26701 tattaactat acttatatac aactctggat ttattgtagt ctgtgcaaca agacaatatt
    26761 ttttggagtt attaaattcc atattttcca tttgttctaa ggtctttatt attatagctg
    26821 aattttcaca ccatccattt atacctataa cttctggatg cttcatatct ccaataacta
    26881 ttatttcata gcctttatta tgatattcac ttactactgt atgtattttc tttacaaatg
    26941 gacatgtagt atccacaatt tttaattttt tatctttggc ttccttatat atattttcag
    27001 gaactccatg agaccttatt atcatatttt catgatttgg tattgtatca atttcattca
    27061 cagtcttaag acctttttct tcatattttg ctactgcttg cttgttatga ataagaggac
    27121 ctaaagcata tatgccagaa tcattttttt caacttcatc ccatgccatt ttcattgctc
    27181 tttttactcc aaaacaaaag ccagcatttt ttgctatttt tatgttcata actttacccc
    27241 cttattttga atatttaaag taattttgtt ttatcacttc taaaatctct tgtgatatcc
    27301 tttcatggtc atcattactc aatttttcct taaaatgttc ttcaaatgat attggttctc
    27361 caatatatac aattacttta ctaaaaagtt tatatttaga tattattgat actggaacaa
    27421 ctttagattt actttttatt gtaaatacag aaagacctgc cttagcctta ccaaaagaat
    27481 ctccttttat tctagtaccc tctggaaata ttcctaatac ataaccatct ctaactgacc
    27541 ttaaaatagt ttttattgta gatacatctg gtttttctct atttataggc ataacatgca
    27601 atttttttaa tataaatccc aatggcttta ctttaaacaa ttctttcttt gcaattgcag
    27661 ctatttccct attttctatg gcagcagcca agaatatagg gtcaagattc gatttatgat
    27721 ttgatgctat tactatattt cctctatctg gaatattctc tgcacctata acttcatatt
    27781 taaaaaatat tttagaaaaa cccttaaaaa tatttattac aaatctataa aaattcacgt
    27841 ttatctccct tcaacactac atttattttc attatgatgc ataaaaaact aaagatttat
    27901 tttagataca acttcttcta caacttcttc tatagttttt cctgtagtat ctatttctag
    27961 tgcatcatta gcttttacca atggtgcaaa ttctctattt gaatcaattt catctcgtct
    28021 tattatatcc tcaattactt cttctaaagt agtattatat cctttattac aaagttcttt
    28081 atagcgtcta tttcctcttt cttctggagt agctactaaa aagaatttgt aatctgcatc
    28141 tgggaaaaca tatgaaccta tatctctacc atctagtatt actgaacttt tcattcctat
    28201 ttttcgttga acttcaacca tcaactgtct tacttctttt atttttgcta cattagaaac
    28261 attattgctt acttctattg ttcttatttc ttcatttata acttctttgt ccaaatatat
    28321 attgttatct ttaaaatcta tatccgaatt ttcagctatt tgaataactt ctttttcatt
    28381 attgacatca acaccacttt ttaaacactt ataagtaact gctctgtaca tagcacctgt
    28441 atctatataa tttatattta gcttttttgc tactatcttg gctattgtac tttttccagc
    28501 tcctgctgga ccatctacag ctataactaa attacccata tatacacctc tatttattca
    28561 tatttaatta ttaaaaaatt atatttatac acttataccc gcaagatatc cagttgaaaa
    28621 tgctatttgt aaattatatc caccagtttc tgcatctata tcaattactt cacctgtaaa
    28681 aaataagttc ttaactaatt tagattccat agtagaagaa ttaatttctt taactgaaat
    28741 accaccagaa gtaaccatac cagtgtttat acctgtagtc ccattacaag ttagcttcat
    28801 attttttata tactcaataa ttctaatttc atctgcctta gacaaatcac ttgccttttt
    28861 atcaactaaa gataatagag aaaaaatctc tcttaagaaa ttttgaggta gtatttgctt
    28921 aagattattt agaacatttt tatttggaaa tgctctgatt atactagaga tttcatcagt
    28981 acttaaattt ggtaagaaat ctatatttaa ttctaccttt tccttttcaa taatcttgtt
    29041 aatataagaa gacattataa gaacacaagg tcctgttatc ccaaaatggg caaatagcat
    29101 gtctccagat tttgatattt tccttttctt aatcttacaa gatatttcca cattcttcat
    29161 ggatattcct tgtaaatctt taatccattt ttcctctatt gtaagtggaa ctaaagcagg
    29221 atatagtttg tttaatgtat gtccatgctt tttaagaatc ttatacattg aaccatctga
    29281 acccgtcttt gaataactca ctcctccagt tgatataata accttgtctc ctagtatctt
    29341 atctccatta tctaaaataa tcccttttat tagatacttt gacttgtcct tattagaatc
    29401 atcttttaga gcaatttctt cagttataat atctataact ttcttattat acattatctt
    29461 tacattgtga ttgagtaaat ctttatttaa tacttctatt acttctatag atttatcatt
    29521 tttagtgtat actttgtggt cattttcttt ttcaatctta tattccaaat ttctactttc
    29581 aaaatacgaa ataaggtctt tatttgtaaa tgtatagaaa ctactataga gaaatttttt
    29641 attactgaca actttatcaa aaaaatcttc tatctctcta ttattggtaa aattgcatct
    29701 accgccacca gttgccctaa gttttcttcc taattctcca tttctctcaa ctaatattac
    29761 ttcattattg ttttttgaag ctgacaaagc tgccatcatt ccagaagctc ctccaccaac
    29821 tactataact ttactcataa atttctcctt ataattgatt cctactccag tgcagaatta
    29881 tttatcttta catgattttt tatatgactt gtatcattca tttttactat aacaggtcca
    29941 taatctttaa attctactat atttaaagca gtattatctt gcttaattct atatatattt
    30001 tccatacctg attctaaaaa tgacaacaac ataaccctca atgtaattga atgtgtaaca
    30061 agaattatat ttttattatc atatttttca tttagttcct ttataaaagc atctactctt
    30121 tcctttataa tcttaagagt ttcgccttct ggtatattta ctaagtgtgg ttcatttctc
    30181 catgttgcat atatatctga ataatctttt tgaatttctt ttattaaaag gccttcccaa
    30241 actccgaatc ccatttctct aagtgcctca gttttttgaa cttcaatatt gagcttatct
    30301 cctaaaattt gagctgtttg cattgctctc ccaaggtcgc tactaaaaat ataatcaatt
    30361 gaatacttgc caatatcttc agaaagttct tttgcctgtt ctataccttg aggcgtaagg
    30421 tcagaattac catgaccttg tgtttttccc aatatattcc aatccgtttg tccatgtctt
    30481 actatataaa aagtatttcc catatgtcct cctaatatat gcctattttt tattaaatat
    30541 aaattatcac ttctcaaaac ttaattatat catataatac tttactttgt ttctatttta
    30601 taatctaatc taacatatta tttacaataa atctcatttg tatttctgca aattattgta
    30661 ctgttaaaga atttttctaa atttataaac atatttctct tatattattt atatacttct
    30721 tcaaatctgt attcttgaat tgaattgaca tacttatctg tatctacttt ttctaaaaac
    30781 atatccaaag gtcttgcaaa tattcctgta tcatcatata aggtcttgta taaaactaaa
    30841 tcattagtat cttcttcttt tttatgataa aggtttccat ctaaatccct ataaacatag
    30901 atatcttctt ctctttcagt atgtctaata actaatttat atttatttct gttttggatt
    30961 aaattttctt taccatatat ttcacatatg ttttctatat catcaatagc cttgctaaga
    31021 cccattgtag cataatattt ccctttaaaa tgtttataga tacaaggata ttttaacttt
    31081 ctttcactga tacctaagtt attctttaat tcaatcattt tattataatc tcccatatct
    31141 cgtccctttc ttttaaaaag tattttattc tgaaataagt tacttgtttt atattttatc
    31201 ataatttaaa atttattaat aatacaatca ataaataaag aggctgtata aaatacagcc
    31261 tcccaatatt aatcttcata atcatataat ttttctaatt tcttttttaa tttctttttt
    31321 ctttctgaac ctaaaattct tataaaatta ttattcacaa aaatacaatt attgctgaga
    31381 taaaattcac acttgtctag atttacaata taactattac tgcatttgaa aaaattttct
    31441 tctttcaata gttttcttat acaaggagca ttttctccaa cttcataatt accactttca
    31501 gtataaatct ttacttcata ctcttgcttt tctatgtaca aaacttcttt actattaatt
    31561 gaaaaacgtt tatcctcgta ttcaacatta ataaaatttt cctctttcat ttccatccct
    31621 ccaagtactt ttattaattt aattttagtg ttttttatca atataatcta tcggaaatat
    31681 atgtaagact gatttactta agatatttaa ataattatta ttaatattaa tgtataagtt
    31741 tatttttta
//
LOCUS       DAEPLF010000025        28968 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEPLF010000025 DAEPLF010000000
VERSION     DAEPLF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28968
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.025"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            495..3155
                     /locus_tag="KR314_001018"
     CDS             495..3155
                     /locus_tag="KR314_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBH0327416.1"
                     /translation="MKKILKRLCTGFLAFATVVTALPSSTVHASNTQYWTESKERVSI
                     VEQVMNDGSISSTFNEGHLTVEGEDAYCIDINTAFKNGYKTKADASTRMSADQIADVA
                     LSIEYVKQYTDIHSGISSKHAYLLRQLVVWQRLSVQPDWQWDNVRASYDEIPKAVQDE
                     VFAGAKAFVKENKGRYDCGGYIYSGEGQELGQFWAKLAVGNTKLQKTSTNANITDGNG
                     IYSIAGATYGVYSDKDCTKQLTTLTTDTSGNTEAVEVRATIVYIKELSAPAGFKIDKT
                     VYSLSVEVGKTATLKVSDTPKVTDTLIELFKIDMETQKSNPQGNASLEGAEFTWNFYA
                     GYYNKNNLPAQPTRTWVTKTIAEKDSDGAIHYITRLADKYKVSWDSFYTQDGKNVLPL
                     GTLTVEETKSPSGYLLEGTYMQADGSEEQIKGMYLTQITEDGDLAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETKDTKAQGGATLKDTAFEIISLNENAVLVEGKLYKKNEVLKTIH
                     TDIEGIASTSADLLPYGKYRLSEQKPPEGYLTDGAKPIDFEITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGSHKRLADVPFRITSKTTGESHVVVTDDNGQFSTASDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALLYDTYIIEELRCESNKGFKLIPPFEIVISRN
                     KVVVDLGTLTDEYEKEITIHTTATSKDGEKTILAGKDVTITDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIINDKRIENDYIFTADSETMEVKIEFTFDASNLGGKQLVTFEELYD
                     LSNPDEPIKVAEHKDIEDDGQTVTIKEVPETPTPEEPEKPTTPDTPTKTDSPKTGDNT
                     NILAFAIMMFVSAGGLAGTYFFKHRKMKKS"
     gene            3380..3703
                     /locus_tag="KR314_001019"
     CDS             3380..3703
                     /locus_tag="KR314_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBH0327417.1"
                     /translation="MELKYIVPNVAQTFGNLEFAGENKVDQQRINGRYTLVSRSYNLY
                     SDIQRADDIIVVLPAKAGEKHFKPEQKVKLINPRITAEGYNVKGRGFTNYILMADDML
                     PAESK"
     gene            3718..4101
                     /locus_tag="KR314_001020"
     CDS             3718..4101
                     /locus_tag="KR314_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBH0327418.1"
                     /translation="MRLTNGIVLDNEKTFGVLKFSALRHEVRVENEDGTTSDEIKRRT
                     YDLKCSVQERMIQVSIPADVPVKDFPYNAEVELINPVADTVANANFRGTTVDWYIKAD
                     DIVLKNKGGQAGNPQNNAPQGQQKK"
     gene            4197..4610
                     /locus_tag="KR314_001021"
     CDS             4197..4610
                     /locus_tag="KR314_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBH0327419.1"
                     /translation="MLDKIPNAEEMTALVGQSLYSVWNELCALIDEKYEMECLWNKGG
                     KAWNYEYKYRRGGKTLCALYARENCVGFMIILGKDERLKFDTYRDNYSKEVQRIYDET
                     KTYHDGKWLMFEPTDTTLFDDFVKLLRIKRKPNRK"
     gene            4717..5175
                     /locus_tag="KR314_001022"
     CDS             4717..5175
                     /locus_tag="KR314_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0327420.1"
                     /translation="MKLKNPMLVVTDIDKSVEFYKKVFGLYVIMDFGANKTLTGGLAL
                     QTSETYKEFIGTSDISFGGNNFEVYFEEEDFDKFADRLKEYDIEYVHPIIEHSWGQRV
                     VRFYDPDKHIIEVGENMKIVCKRFLNSGMTPEQVAERMDVPMKFINACMR"
     gene            5200..5742
                     /locus_tag="KR314_001023"
     CDS             5200..5742
                     /locus_tag="KR314_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBH0327421.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNYCGGCGNGNQSCRIAKC
                     SLEHGQIEYCYECKQYPCEKYQHIDDYDSFITHKRRKADLERAKNIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EVFQKIAERRNIKLKLFKKK"
     gene            5778..6305
                     /locus_tag="KR314_001024"
     CDS             5778..6305
                     /locus_tag="KR314_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HBH0327422.1"
                     /translation="MTNGGIPLETVELRFQYTQSEYIKAERQYLISSKTIHKYDIALV
                     AVFLLLSVVYMLFSSFSVFSILIFGLIIVVTALGSYLYILMPILKFKQTAKYHEEYTL
                     VFSKENIKFKTQSIDSEVKWDIYSALWESHDFYYLIQAPRLYTLIPKRIFKDLNEKQL
                     FEEIAQSRVKTTKHI"
     gene            6415..7809
                     /locus_tag="KR314_001025"
     CDS             6415..7809
                     /locus_tag="KR314_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0327423.1"
                     /translation="MRMILNKGHRIRARDKNLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFSLLDNGLTLSPYNFITILIATGICALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMENGLLHIHCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRISIDEVKAENGCLRLMKNLV
                     WEYDVLPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEDMIDCVNAFYEGMVQRSEEMKQHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAFVRQ
                     DGTATCEAKGDGTD"
     gene            8089..9210
                     /locus_tag="KR314_001026"
     CDS             8089..9210
                     /locus_tag="KR314_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBH0327424.1"
                     /translation="MAGISREHLSRIEAGKVALTEDMKHKLLEAVEKFNPDAPMFLLF
                     DYVRIRFPTLDIQHVIKDILKLNIDYMLHEDFGHYKYTEHYYIGDVFVYTSQDKEKGV
                     LLELKGKGCRQFESYLLAQERSWYDFLMDALVDGGVMKRIDLAINDRTGLLDIPELIE
                     KCKNEECISKFRSFKNYGSGELVKHNEANKDGMGHTLYIGSFTSEVYFCVYEKNYEQS
                     SKLGIPIEEVPIKNRFEIRLKNEHAYYAIRELLTHYDAELTAFSIINQYIRFADKEAN
                     KRKSDWKTNARWSWFIGEGRPPVKLTTKPEPYTLQRTLNWLQRQVAPTLKMLKQIDAG
                     NQMDYLKTIEQQAKLTDKHKQIIRQQTASVDELIEKEKE"
     gene            9217..9351
                     /locus_tag="KR314_001027"
     CDS             9217..9351
                     /locus_tag="KR314_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBH0327425.1"
                     /translation="MWELIKDLLLVSLGMGIGVVLMCIMSVGKEADRHMIELKESEEK
                     "
     gene            9352..9573
                     /locus_tag="KR314_001028"
     CDS             9352..9573
                     /locus_tag="KR314_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327426.1"
                     /translation="MNFGQNLYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALVAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            9893..10531
                     /locus_tag="KR314_001029"
     CDS             9893..10531
                     /locus_tag="KR314_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HBH0327427.1"
                     /translation="MEYKYTKIDLNKWNRGKLFQSYIDNMRIVMSLTVDIDVTKFIEF
                     SKKHDLKFYPAMIWAVSKVVNEHDEFKYSWDNNGNLIKWDYISPSYTEFHKEDENFTK
                     IVTDFSDSIFEFHKRFILDREKYKTERAFVENQSLNFFDVSCLPWVKYNHFDVHVFDE
                     GKFLAPVITWGKYELEQERYIMPLTMNIHHAVADGFHLSRFFNEVQELINSI"
     gene            10608..10796
                     /locus_tag="KR314_001030"
     CDS             10608..10796
                     /locus_tag="KR314_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327428.1"
                     /translation="MKDIFSDMQASIGCTYISDLPHHKRMVWQEMKRLNLADYPQKQL
                     EDFAGYVFGVPYTSIAEA"
     gene            10817..11314
                     /locus_tag="KR314_001031"
     CDS             10817..11314
                     /locus_tag="KR314_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBH0327429.1"
                     /translation="MNEMRVCIESRLHTSEEIRSAWFTLPIDEEELEEQIGVPIDSED
                     YHIVEKELPFADEVGEDATIEKLNDLYHTFESLPADLQEDYGELMCYFTSLDELHQHR
                     HDVIHYSWCKNMADVARHLLDNDPAFSAIEERLTRYFDYEAYGQYLDDNGEFIETDHG
                     IYELP"
     gene            11330..11833
                     /locus_tag="KR314_001032"
     CDS             11330..11833
                     /locus_tag="KR314_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBH0327430.1"
                     /translation="MVEDMRVYIANLGKYNEGYLVGDWFSFPIDEEDVAERIGLNERY
                     EEYAVHDTENFPIEIGEYISIEELNEMYEMICELPDYITDSLDEFVSNYGSLEELYDH
                     RDDMILYAGCEDMTDVAYYFIDELQALGDIPPSLQNYIDYEAYGRDLSFEGTFIETRY
                     GMCEIPW"
     gene            11946..12335
                     /locus_tag="KR314_001033"
     CDS             11946..12335
                     /locus_tag="KR314_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0327431.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDLPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITFALRPKITY
                     AGKAVKLDKQVLHEAITAVRSVSYVPD"
     gene            12322..14754
                     /locus_tag="KR314_001034"
     CDS             12322..14754
                     /locus_tag="KR314_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0327432.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSSRSIQEQSKKLVTGRLKDVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTDQSMNLKSVKQSAFLTFKEFIHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLDKNDFGYLIEHIYGRDGVAYEDYEYSLPKKKLKKE
                     TLITHYDLIRPTRCLVEESQRHLRLEHEDSESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALSTVRNKKKELKDLDNHAYQSGSETSSNVVDALDSVD
                     ELETDLDQSRESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYIGYSVDTGRNVYLQPS
                     LASQGVKDTVTNALASAFVESLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDRENAGLLDPFVIMQNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRAVTQSDQRGLLHVIEELRKEDTPISCNIADHIDSFTDYDFAHLLFSDGTV
                     QNAISLDNQLNIIQVADLVLPDKDTTFELLSVSMLIVISTFALDFIHSDRSVFKIVDL
                     DEAWAFLNVAQGETLSNKLVRAGRAMNAGVYFVTQSSGDVSKESLKNNIGLKFAFRST
                     DINEIKQTLEFFSIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFEELLHAF
                     DTRPPIQRNEVE"
     gene            14754..16889
                     /locus_tag="KR314_001035"
     CDS             14754..16889
                     /locus_tag="KR314_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBH0327433.1"
                     /translation="MKERIKSVFTRKKIFRFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINTENLYSKYPLSNYQLDFYADNNWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     RLYLSNATGYVVQEAYKLDFINDMADSIGRSIQTLAGVTQNGFSTSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKVNDFSSDISTAS
                     LDLGTKIMLPDSDSKGKDSVDLIRDSLFSIQVQQPWLLLQFGNSGIEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTILQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVYFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRLARAVSAGGVAGAVAGGVVASSKSTRQNGTKNNRGNTSSSMGQK
                     AGSAVGAVLDTKNKVKDKATAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGVVQE
                     KENRQSGRLKKQQLHRQNIADKRMELQKAQESRQAGKKTDGSATSGATRPHERPATTS
                     KVNVEKQQEVKRPAIASTVKNDEPIKKNVVKERPLSSGDTSAKTVNQETTRQNYKTDR
                     VTKTTVQKHTQQNTEKNKRTVFKKGQKKK"
     gene            16886..17893
                     /locus_tag="KR314_001036"
     CDS             16886..17893
                     /locus_tag="KR314_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBH0327434.1"
                     /translation="MKLRHIAIVGSLFSILFSLVLFFGVLISAEDDDGGNGYSSNYVG
                     MNLSAEVLKHQPMVEKYAKENGISEYVNVLLVIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGISDINVVIQSYNYGGGYVGYVAGNGKKHMFNLA
                     ENFARDKSGGQKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLVVNQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYNKAGVSLPRTAQNQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNTQMYHAGDPIGYADLNNAYWQQYLICAGRIK
                     Q"
     gene            17909..18802
                     /locus_tag="KR314_001037"
     CDS             17909..18802
                     /locus_tag="KR314_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0327435.1"
                     /translation="MFKKKEKPVKETKVRTMKVGTHKKSVIALWVVLIASVSFGVYKN
                     FTAIDMHTVHETETIQLRLNDTNGVENFVKNFCKSYYTWDNSKEAIEARTQAISNYLT
                     KELQDLNLDTIRTDIPTSSTVTNVLIWDIKQSGTNDFIATYEVDQQIKEGEQTSNVKA
                     TYTVKVHVDKDGDMVIVQNPTIAPAIEKSDYEPKAQEADASVDADTVNDATAFLETFF
                     KLYPTATEKELAYYVAGNVLEPIGRDYLYSELVNPMFTDDRDNVKVRVAVKFIDNQTK
                     AMQVSQFELVLHKDSNWKIIM"
     gene            19015..19872
                     /locus_tag="KR314_001038"
     CDS             19015..19872
                     /locus_tag="KR314_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0327436.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERNSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPAWSHRFMGLIQLGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFRFDYTNKVFDLIHVPKKELLTLELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            19950..20531
                     /locus_tag="KR314_001039"
     CDS             19950..20531
                     /locus_tag="KR314_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0327437.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFSLYGLKKVTVDDLAAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTVKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEGLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMVQNLLVNGIIQQVVAE"
     gene            20635..21480
                     /locus_tag="KR314_001040"
     CDS             20635..21480
                     /locus_tag="KR314_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327438.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KILTGLNTSYKGSVKVAGTEIREHTNRFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     DKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNNRLMKDIILAEKKRGKTIIITTHNMYDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIHYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            21477..22160
                     /locus_tag="KR314_001041"
     CDS             21477..22160
                     /locus_tag="KR314_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0327439.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALTGHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKVSEYILSKTIALIAISTI
                     IAVILGFVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPDIFRFFPFTACMNLITGKSILLSFDMVLVIATLIILYIVARHTVKHM
                     WRSLGGVKL"
     gene            22157..22855
                     /locus_tag="KR314_001042"
     CDS             22157..22855
                     /locus_tag="KR314_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0327440.1"
                     /translation="MKRILSSTIQVFKQIKSDPMMFAACFTPFVMGALIKFGIPFLDG
                     ITDFSLQTYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLMVALVW
                     YYFLIKRLFRKLAG"
     gene            22867..23778
                     /locus_tag="KR314_001043"
     CDS             22867..23778
                     /locus_tag="KR314_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH0327441.1"
                     /translation="MEKGRLLQNENINKAIQVSKITLIINLGLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDKEHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVSSGIALTAAVLSIVIKEWMYWYTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASIVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELPYSP"
     gene            23831..23971
                     /locus_tag="KR314_001044"
     CDS             23831..23971
                     /locus_tag="KR314_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327442.1"
                     /translation="MEQYWWIIIVMVLIVVGLLKIVIMKKYNQRRNSNNRSKNSEDDK
                     MY"
     gene            24086..24277
                     /locus_tag="KR314_001045"
     CDS             24086..24277
                     /locus_tag="KR314_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBH0327443.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            24301..24531
                     /locus_tag="KR314_001046"
     CDS             24301..24531
                     /locus_tag="KR314_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327444.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            24797..25087
                     /locus_tag="KR314_001047"
     CDS             24797..25087
                     /locus_tag="KR314_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327445.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELRRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKNVTMY"
     gene            25087..25221
                     /locus_tag="KR314_001048"
     CDS             25087..25221
                     /locus_tag="KR314_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0327446.1"
                     /translation="MYYFLEMSDTEIADIMNYTREAVFKHRHRALETMKEILNEKTRQ
                     "
     gene            25202..26383
                     /locus_tag="KR314_001049"
     CDS             25202..26383
                     /locus_tag="KR314_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH0327447.1"
                     /translation="MKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYSWKLVP
                     TDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRNTEL
                     GRQYLVNALENDPLGMRAIDTVKQSDAKEWAIRMNGKGYSYKTIDNYKRSLKASFYMA
                     IQDDCIRKNPFNSKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVILLL
                     ETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISKAKTKNGKRELPLTERAY
                     QALKRILKNRGKAQPLVVGGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHKDKL
                     PNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELERLA
                     S"
     gene            complement(26723..27535)
                     /locus_tag="KR314_001050"
     CDS             complement(26723..27535)
                     /locus_tag="KR314_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0327448.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRIL"
     gene            complement(27611..28846)
                     /locus_tag="KR314_001051"
     CDS             complement(27611..28846)
                     /locus_tag="KR314_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBH0327449.1"
                     /translation="MGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITSGVTGMF
                     CHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLRGSLASLLEVESGSNDPIAY
                     MLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFYTIF
                     ITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIMLFF
                     MLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVGLRG
                     AASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTPVLK
                     TFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGSTII
                     KTGDILVLSGDRIEELIQL"
BASE COUNT         9447 a         5180 c         6054 g         8287 t
ORIGIN      
        1 tacttaaaat taaataaagt attactttaa atacccaata tctttttttc ttgatactgt
       61 atattttttt actttatatc cttaaaaaaa tatacaactt tatatataaa acatcaatat
      121 ttatatatat tttgtatatt aaatacaatt atttataaaa tctttgataa aaatttataa
      181 atagagcatc tttagaaaac tattaacaga aaaacacact aaaaatttga ttatccgcta
      241 aaacactgta tctatcggca ttatcaagga gctaaacaca tcaaaatact actaatttac
      301 tacgattgaa tgagccttga tacgtcctat ttcccagata tgcaaagata cggtacagca
      361 catcagtatt gaaacaagaa aaattgaata cttcatagac tacggaacgc acaatatcag
      421 cgttcctttt ctatttccag ccgttcctag tggacggcta ttttactacc aaaatgaaag
      481 gagcatttag aattatgaaa aagatattaa aacgattgtg tacaggcttt cttgcctttg
      541 caactgtcgt taccgcttta ccgagttcaa cggttcatgc gtctaataca cagtattgga
      601 cggaaagtaa agagcgtgtc agtatcgtag aacaagtaat gaatgacggt tctatcagtt
      661 ctacctttaa tgagggacat ttaaccgttg agggtgagga cgcttattgt atcgacatca
      721 acacagcctt taaaaacggc tataagacca aagctgacgc aagtacccgt atgagtgccg
      781 accagatagc tgacgtcgcc ttatctattg agtatgtgaa acagtacaca gacatccaca
      841 gcggaataag cagtaaacac gcctacctat taagacagct tgttgtctgg cagagattaa
      901 gtgttcagcc tgactggcaa tgggataatg tgcgagcttc ctatgatgaa atcccaaaag
      961 ccgtacaaga tgaagtattc gcaggagcaa aagcctttgt gaaagaaaac aaaggacgtt
     1021 atgactgtgg cggatatatc tactctggcg agggtcaaga gttagggcaa ttctgggcga
     1081 agttagctgt cggaaatacc aaacttcaaa agacttccac caatgccaat attacagacg
     1141 gcaacgggat ttattcaatc gctggtgcaa catacggtgt gtattctgat aaagactgca
     1201 cgaaacagct taccaccctt acgactgata ctagcggaaa tacagaagcc gttgaagtaa
     1261 gagccactat agtatatatc aaggaacttt ctgcaccagc aggatttaaa attgataaaa
     1321 cagtttactc tttatctgtg gaagttggaa aaacagcgac tttaaaagta tctgatacac
     1381 caaaagtaac agataccttg attgagctat tcaagattga catggaaaca cagaaatcca
     1441 atccgcaggg aaacgcttct ttagagggag cagagtttac atggaacttc tacgctggat
     1501 attacaacaa aaacaatctt ccagcacagc ctacaagaac atgggtaaca aagactatcg
     1561 ctgaaaaaga cagcgacggt gctatccatt acattactcg tttagctgac aaatataaag
     1621 tatcttggga cagcttctat acgcaggacg gtaaaaacgt acttccatta ggaacactaa
     1681 ctgttgagga aacaaaaagt ccgtcgggtt acttgttaga gggtacatat atgcaggctg
     1741 acggtagcga agaacagata aaaggaatgt atctgacaca gattacagag gacggcgacc
     1801 ttgccgtatt atctggaagc aatcaatacc atgtttctga taaagtcatt cgtggcggtg
     1861 tgaaaatcca gaaacgagat ttagaaacca aagacacgaa agctcaaggc ggtgcgactt
     1921 tgaaagatac cgcctttgaa atcatttcct taaatgagaa tgccgtctta gttgagggca
     1981 agttatacaa gaaaaatgaa gtgttaaaga caatccatac cgacattgag ggtatcgctt
     2041 ctacttctgc tgacctatta ccttacggaa aatacagatt gtctgaacaa aaaccgccag
     2101 agggatattt gactgacggt gcaaagccga ttgattttga aatcacagaa aatggaaaaa
     2161 tcgttgattt gactgacgaa gcccactcta tctataacca gattaaacgt ggcgatattg
     2221 agggcgtaaa aattggtgca ggttcgcaca agcgtcttgc agatgttccg tttaggatca
     2281 caagcaagac gacgggcgaa agtcatgttg ttgtaactga tgataacgga caattctcta
     2341 ctgcttctga ttgggcttcc cacaagcaca ataccaatgc aggaaaaaca agcgaggacg
     2401 gtgtgtggtt tggtacttct gaaccagacg acagcaaagg tgcgttgctt tatgatactt
     2461 acatcattga agaattacgt tgtgaaagca acaaaggttt caaacttatt ccaccgtttg
     2521 agattgtcat ttccagaaat aaggttgtgg ttgatttagg tacgctgact gatgaatacg
     2581 aaaaagaaat cactatccat actactgcta ccagcaagga cggcgaaaag actattcttg
     2641 ctgggaaaga cgtaactatc actgatacag ttaaattaga cggtcttacc aaagggacaa
     2701 aatatcagtt gaaaggctgg cagatgttaa aagaagaaaa tgcagagctt atcatcaatg
     2761 acaagcgtat tgagaatgac tacatcttta cggctgacag cgaaactatg gaagttaaaa
     2821 tcgagtttac ctttgacgct tccaatcttg gcggtaaaca gcttgtaact tttgaggaat
     2881 tatacgattt aagcaatcca gacgaaccta tcaaagttgc ggaacacaag gacattgagg
     2941 acgacggaca gacagttact atcaaggaag taccagaaac acctacccca gaagaaccag
     3001 agaagccaac cacaccagat acacctacga aaacagacag tcccaaaacg ggcgacaata
     3061 caaatatcct tgcctttgca attatgatgt ttgtttctgc tggtgggctg gctggtacat
     3121 actttttcaa acaccgtaag atgaagaaat cataaggcgg tagctgtatg agaaaagaaa
     3181 aatcacgctc tccgccatag cttagatagg cagactatac ctattctggc ttgtctgccg
     3241 attacctgca agaacacgga tagtcaaggg tgcgttagca tttatttacc ctttactatc
     3301 tgggggcttg ctggcacttt cctaaatgcc agaatacaca catcaatcaa acttaaagta
     3361 agaaacgagg taaaataata tggaattaaa atatatcgtg ccgaatgtgg cacagacttt
     3421 tggaaacttg gaatttgcag gggaaaacaa agtagaccag caacgtatca atgggcgtta
     3481 cactctcgta tcaagaagtt acaacttgta ttcggatatt cagagagccg acgacattat
     3541 cgttgtattg cctgcaaaag ctggcgagaa gcattttaag ccagagcaga aagtaaaact
     3601 gattaaccca cgcattaccg cagagggtta caacgtcaaa ggacgtggat ttacaaacta
     3661 cattttaatg gctgatgata tgttaccagc agaaagcaaa taagggggta tttgattatg
     3721 agattaacaa atggaattgt attagataac gaaaaaactt ttggagtatt gaaattctct
     3781 gcacttcgac atgaggtgcg tgtagaaaat gaggacggaa cgacaagtga tgaaattaag
     3841 cgtcgtactt atgatttgaa atgtagtgtg caggaacgca tgatacaagt gtcaatcccc
     3901 gctgatgttc ccgtaaagga ctttccttat aatgcggaag tggagttaat caatcccgta
     3961 gctgatacgg tggcaaatgc caactttaga gggactacgg ttgattggta catcaaagct
     4021 gatgatattg tattgaagaa caaaggcggt caagctggaa atccgcagaa caacgctccg
     4081 cagggacagc agaaaaagta agctgacaaa ctagtttttc attgtaggag atataaacaa
     4141 ctgttataat atggatgtgg aaacagttaa tttagactta taaaggagaa atgtgtatgc
     4201 tagataaaat acctaatgca gaggaaatga ctgctttggt cggacaatca ttatatagtg
     4261 tatggaatga attgtgtgcc ttgattgatg aaaagtatga aatggaatgt ctatggaata
     4321 aaggtggcaa agcatggaat tatgaatata agtatcgtcg aggtggcaaa acattatgtg
     4381 ccttatatgc tagagaaaat tgtgtgggat ttatgattat cttaggtaaa gatgaacggt
     4441 taaaattcga tacatataga gataattatt caaaagaagt tcagagaatt tatgatgaaa
     4501 caaaaactta ccatgacgga aaatggctga tgtttgagcc aacagataca actttgtttg
     4561 acgattttgt taaacttttg aggataaaaa gaaaaccaaa tagaaagtag tttattacca
     4621 caccattagc aaatggatag tttcttataa tagcggatat atgaaaatgc tgtcatgtgg
     4681 acataaagaa aattggaagt tgtggaggta gcaaaaatga aattaaaaaa ccctatgttg
     4741 gtagtaactg atatagataa atcagttgag ttttataaaa aggtttttgg actttatgtc
     4801 attatggatt ttggggcaaa taaaacttta acaggtggat tagccttaca gacaagtgaa
     4861 acatacaaag agtttatcgg tacaagtgat atttcttttg gtggtaacaa ttttgaagtc
     4921 tattttgaag aagaagattt tgataaattt gctgataggc tgaaagaata tgatattgaa
     4981 tatgtccacc ctattataga acattcatgg ggacagcgtg tagtccgctt ttatgaccca
     5041 gataaacata ttatcgaagt tggagaaaac atgaagatag tgtgcaagcg tttcttaaat
     5101 agtggaatga caccagaaca agttgcagaa cgtatggatg tacctatgaa atttataaat
     5161 gcttgtatgc gataaattga aagttaggag actaatacta tgaaaggatt tgaacgcaaa
     5221 aatcaattat tttctctttg cggattgaat tgcggactat gccctatgct tttgggaaat
     5281 tactgtggcg gttgtggcaa tggtaatcaa tcatgcagaa ttgcaaaatg tagtcttgaa
     5341 catggacaga ttgaatattg ctatgaatgc aaacaatatc catgcgaaaa atatcaacac
     5401 attgatgatt atgattcttt catcacgcat aaacggcgaa aagcagattt agaaagagca
     5461 aaaaacatag gcattgagca atataatctt gaacagcagg aaaaaacaca aattctttct
     5521 tacttacttt ctaactataa tgacgggcgt agaaagactt tcttttgtgt ggcagtcaat
     5581 ttattagaac tttcagaatt acaagaagca atgaaacaaa tacaatccaa tgacgaattg
     5641 accttactac catttaaggc acaatgcttg tatgtggtgg aggtgtttca gaagattgct
     5701 gaaaggagaa atataaaact gaaacttttc aagaaaaagt gattttcaaa tttgaaattg
     5761 taacaaagat tatttatatg acgaatgggg gaataccttt ggaaacagta gagttaagat
     5821 ttcaatatac tcaaagtgaa tatataaaag cagaaaggca atatttaatt tctagtaaaa
     5881 ctatacataa atatgatatt gctttagtcg ctgtattttt gttgctgtca gtcgtttata
     5941 tgctgttctc ctcttttagc gtatttagta ttttaatatt tggattgata attgttgtaa
     6001 ctgcattggg aagctattta tatatcctaa tgcctatact gaaattcaaa caaactgcaa
     6061 aatatcatga ggaatatacg ctggtatttt ctaaggaaaa cattaaattt aaaacacaaa
     6121 gcattgactc agaagtgaag tgggacattt actctgcact atgggaaagc catgattttt
     6181 attacttaat tcaagcacca cgattatata cgttaattcc caaaagaatt tttaaagacc
     6241 taaacgaaaa acagctattt gaagaaattg cacaatcaag agttaaaaca acaaagcata
     6301 tataatgaac ataccctaca atgtaacaac ttcatattca tacacacaaa gcggttagtc
     6361 ttttaagatt gactgctttt ttcatactta aattaagatt ggagtgattg atttatgcga
     6421 atgattttga ataaaggaca ccgtatcaga gccagagaca agaaccttgt ctaccacttt
     6481 tccataggga cgcttctgtt tgtatttgtg gcggttctcc tgctactgaa tatcaaacag
     6541 ctcatgcgta ccgactggga acatttcagc ttattggaca atggcttgac gctttccccc
     6601 tacaacttca taaccatatt gatagcgact ggtatttgtg cattggtcgc ttttctgtat
     6661 taccgcttct gttatgacag tttcaaaaag ctcctgcacc gtcaaaagct ggcacgaatg
     6721 atactggaaa acaagtggta tgaagcagat accgtacaag acagcggttt ttttactgac
     6781 ctgcaaagca gatcaaggga aaaaatcgtc tggtttccaa agatttatta tcaaatggaa
     6841 aacggattgc ttcatattca ctgtgaaatc acattaggaa aatatcaaga ccagcttttg
     6901 cgattagagg ataagctgga aagcggtttg tactgtgagc tgaccgacaa gaccttgcat
     6961 gacggctata ttgagtacac gcttttatat gacatgatag cgaaccgtat ttccattgac
     7021 gaagtgaaag cagaaaacgg ctgtcttagg ctgatgaaaa atcttgtctg ggaatatgac
     7081 gtactccctc acgctctgat agctggtggg actggtggcg gtaagaccta ctttctgctg
     7141 acgcttattg aagctctgct acatacgaat gctgtccttt acatcttaga cccaaagaac
     7201 gctgaccttg cagacttagg tacagttatg ggaaatgtgt atcataccaa agaagatatg
     7261 attgattgtg tcaatgcctt ttatgagggc atggtacaaa gaagcgaaga aatgaagcaa
     7321 cacccagact ataaaacggg cgaaaactac gcctatcttg gtttaccgcc ctgctttctt
     7381 atctttgatg aatatgtggc gttttttgaa atgctgggga caaaggaaag cgtaagttta
     7441 cttagccagt taaagaaaat cgttatgttg ggacgacaag ctggatattt ccttattgtt
     7501 gcctgccagc gtccagacgc aaaatatttc tcggacggta tcagagataa tttcaatttc
     7561 cgtgttggat tggggcgtat cagcgaattg ggttacggta tgctctttgg ttctgatgtg
     7621 aaaaaacagt ttttccagaa gcgtatcaag gggcgtggct attgtgatgt gggaacaagt
     7681 gtcataagtg agttttacac gcctttagtc ccgaaaggac atgatttttt gcagaccata
     7741 ggctctcttg cattcgtaag gcaggacggg acggcgacgt gcgaagcgaa aggcgacggc
     7801 acggactagc cgttgctggt gtggcgttag ccacgccagc agatataagc ccctcattat
     7861 ctaacagagg ggcacaatta cgctggaaac tggttcaaaa accttgaaaa gtccgctagg
     7921 aataaggatt ttcttagttt ttagctagtt gtgataatca cttgaaaata tcacaaaatc
     7981 acaacaatat cacgattggg ggtatgacaa ctgaatgata ctgattttct gaacaccttg
     8041 aaagcaaaac gcagtgatta tggcgtatcg cagacaaggc ttgcaatcat ggctggtatc
     8101 agccgtgaac atctaagccg tatcgaagct gggaaggtag cactgaccga ggatatgaag
     8161 cacaaactac tggaagcggt ggaaaaattc aatcccgacg ctcccatgtt cctgctcttt
     8221 gattatgtgc gtatccgatt tcccacgctg gatatacagc acgttatcaa ggacattcta
     8281 aagctgaata tagattatat gcttcacgaa gatttcgggc attacaagta caccgagcat
     8341 tactatatcg gggacgtgtt cgtttacact tcacaagaca aggaaaaagg tgtcctgctg
     8401 gaactgaaag gaaaaggctg tcgtcagttt gaaagctatc tgctggcaca ggaaagaagc
     8461 tggtatgact tcctcatgga cgcattagtt gatggcggtg tgatgaagcg tatcgaccta
     8521 gctatcaacg acagaacggg cttactggat attccagaac tgatagagaa atgcaagaac
     8581 gaggaatgta tttctaaatt ccgtagcttc aagaactatg gttctgggga gctggtaaag
     8641 cacaacgaag ccaacaagga cggcatggga catacgcttt acattggttc atttaccagt
     8701 gaagtgtact tctgtgtcta tgaaaagaat tatgaacaat cctcaaaatt agggataccg
     8761 attgaggaag tccccattaa aaaccgattt gagatacgtt tgaaaaatga gcacgcctac
     8821 tatgccatta gagagctgct gacccactac gacgcagagc tgacggcatt ttctattatc
     8881 aaccagtata tccgctttgc cgataaggaa gccaacaagc gaaaaagcga ctggaaaacc
     8941 aacgcccgct ggtcttggtt cattggcgag ggacgaccgc ccgtgaagct gacgacaaag
     9001 ccagagcctt acactctgca aaggacgctg aactggttac aaagacaggt tgcaccgacc
     9061 ttgaaaatgc tgaaacagat agacgctggc aatcagatgg actatctcaa gaccattgaa
     9121 cagcaggcaa agctgacaga taagcacaag cagataatca gacagcagac ggcttcggtg
     9181 gacgaactga tagaaaaaga aaaggagtga ttgatgatgt gggaattgat taaggactta
     9241 ctgctggtat cacttggcat gggtatcggc gtggttctga tgtgtattat gagtgtcgga
     9301 aaggaagctg accgacacat gattgaatta aaagaaagcg aggaaaaata aatgaatttc
     9361 gggcaaaatt tatatacatg gtttttaagc aacgcccaga gcttagtgct tatggcgatt
     9421 gtggttattg gtatctactt agggtttaaa cgtgagtttt caaaacttat cggcttcttg
     9481 gtggttgcct tagttgctgt tggattggta ttcaatgcag gcggtgtcaa agacgtactc
     9541 ttagaactat tcaataagat tatcggcgca tagccgtact ttttcattat ggtttgcgga
     9601 tacgcaaaca tacctttccc actttgggag tgggctaagg ggaaaggggg taacggaatg
     9661 gtacacagat aaaaatacag cataatgaat ttcattgctt ggctcaaaca tagttgatat
     9721 aataaagata tgaaaataat aaacgaaagt ttttttgata aggagtgata ttgtgaaatt
     9781 gtgtattgtt ttatctccat ataaggctta atggaataga cttgtatgga gtgaaatgtt
     9841 atccagagag ccttacattt tatcaaattt caaaatgtaa aggagaatgg gcatggaata
     9901 taaatataca aaaattgatt tgaataaatg gaatagaggt aaattgtttc aaagctacat
     9961 tgataatatg cgtattgtta tgagcttaac cgtagatatt gatgtaacaa agtttattga
    10021 attttcaaag aagcatgatt taaaatttta tccagctatg atatgggctg tttcaaaggt
    10081 tgttaatgaa catgatgagt tcaaatatag ttgggataat aatgggaatt taattaagtg
    10141 ggattatatt tctccgtcct atactgaatt tcataaagaa gatgaaaatt tcacaaagat
    10201 agttacagat ttttctgata gcatatttga atttcataaa cgttttatat tagataggga
    10261 gaagtataaa acagaacgtg catttgtgga aaatcaatct ttgaactttt ttgatgtatc
    10321 ttgtttaccg tgggtaaaat ataatcattt tgatgttcat gtttttgacg agggaaaatt
    10381 tcttgcacca gttattactt ggggcaaata tgaattagag caagaaagat atataatgcc
    10441 tcttacaatg aatatacatc atgccgtagc agatggattc catttatcca gattttttaa
    10501 tgaagtacaa gaattgataa actcaattta acagctatat acaaagcagt tagtctatac
    10561 gattgactgc tttttttcat acctaaattt aagattggag tgattgcttg aaagatatat
    10621 tttcggatat gcaggcaagt atcggctgta cctacatttc agatttacca catcataaac
    10681 gtatggtgtg gcaggaaatg aagcgactga accttgccga ttatccgcag aaacaattag
    10741 aagattttgc aggctatgtc tttggtgtcc cttacactag catagctgaa gcatgaaaga
    10801 aaggacgtga aaacctatga atgaaatgcg tgtctgtatc gaaagccgac tacacacttc
    10861 tgaagaaatc cgttccgcat ggttcacact tcccattgac gaggaagaac tggaagaaca
    10921 gataggcgtt cctatcgaca gtgaagatta ccatatcgtt gaaaaggaac tgccctttgc
    10981 tgatgaagtc ggcgaagatg cgaccattga aaaactgaat gacctttacc atacctttga
    11041 aagtctgcct gccgatttac aagaggacta cggggagctg atgtgttact ttaccagcct
    11101 tgacgaactg caccagcacc gccatgatgt gatacattat tcatggtgta aaaacatggc
    11161 tgatgtggca aggcatttgt tggacaatga ccctgctttt tctgccatcg aggaacgcct
    11221 gacccgttac tttgattatg aagcctacgg acagtatctt gatgataacg gagaatttat
    11281 agaaacagac cacggtatct atgaactgcc gtagaagtga ggtgtttcta tggtagagga
    11341 tatgcgtgta tatatcgcta atcttgggaa atacaatgag gggtatctgg tcggcgactg
    11401 gttctctttt ccgattgacg aagaagatgt tgccgagcgt atcggcttga atgaacggta
    11461 cgaagaatac gccgtacatg atacggaaaa ctttcccatt gagattggcg aatatatctc
    11521 gattgaagag ctcaatgaaa tgtatgaaat gatatgtgag cttcccgact acatcacgga
    11581 cagtttggac gaatttgtat caaattatgg aagtttggag gaactgtacg accataggga
    11641 cgatatgatt ttatacgctg gctgtgagga tatgaccgac gttgcctatt acttcattga
    11701 tgaattgcag gcacttggcg atattccccc tagtttgcag aactatattg actatgaagc
    11761 atacggcagg gatttatcct ttgagggaac ttttattgaa acaagatacg gtatgtgtga
    11821 gataccgtgg taagaagctg gaagatcagc gacaagcatt gttgcttctg acagcttttt
    11881 ctattgggga cagtctgaaa aacggctgtc cttttctatt atacgaaagg agttgaaaca
    11941 aaaacgtgaa aaagattaaa agctacacgg gtatctggaa cgtggaaaaa gtcttgtatg
    12001 caatcaatga ctttaacctg ccctttcctg ttacctttac acagattaca tggtttgtga
    12061 taaccgagtt tatcatcatt ctgtttgggg acttaccgcc cttatccatg attgaggggg
    12121 catttttaaa atacttcggt attcccgtag ctcttacatg gttcatgtcg caaaagacct
    12181 ttgacggcaa gaagccgtac agctttttga aatcacagat aacctttgcc ctacgaccaa
    12241 agattactta tgcaggaaaa gccgtgaaac tggataagca ggtactacat gaagctatta
    12301 cggcagtaag gagtgtgagc tatgttcccg attaaatata tcgacaacaa ccttgtctgg
    12361 aacaaggaca atgaggtgtt cgcctactat gagctgatac cttacaatta cagttttcta
    12421 tccgctgaac agaaattcat tgtgcatgac agcttccgac agctaatcgc acaatcccgt
    12481 gagggcaaga tacacgcttt gcagattgcc acagaaagct caagccgaag catacaagaa
    12541 cagtcaaaga agctggttac gggacgacta aaagacgttg cctatcagaa gatagacgaa
    12601 cagaccgaag cgttagtatc tatgattggg gacaatcaag tggactaccg cttttttctt
    12661 ggcttcaagc tcatggtaac agaccagagc atgaacctaa agagcgtgaa gcaatcggcg
    12721 tttctcacat tcaaggagtt tatccatgaa gtgaaccaca cgctgatgaa tgactttgtt
    12781 tccatgccaa atgatgaaat caaccgctac atgaaaatgg aaaagctgtt ggaaaataaa
    12841 ataagccgac gcttcaaagt aagacgctta gataaaaatg acttcggcta tctgattgaa
    12901 cacatctacg gcagggacgg tgtcgcctat gaagattatg agtatagctt accgaagaag
    12961 aaactgaaaa aggaaacgct gataacgcat tatgacttga tacgtccgac ccgttgcctt
    13021 gtggaagaaa gccagcgaca tttacgcttg gaacatgagg acagcgaaag ctacgtttcc
    13081 tactttactg tcaatgcgat tgtcggcgag cttgattttc cgtccagtga aatcttctat
    13141 ttccagcaac agcagtttac ttttcccgtt gataccagca tgaatgtaga aatcgtggaa
    13201 aaccgaaaag cgttatcgac ggttcgcaac aagaaaaaag agctgaaaga tttagacaat
    13261 catgcatacc agtctggcag tgaaaccagc tcaaatgtgg tagacgcatt agacagtgtg
    13321 gacgagctgg aaacggattt agaccagtcc agagaaagta tgtataagct ctcttatgtt
    13381 atccgtgtat ctgctccaga ccttgacgag ttgaaaagac gctgtgatga agtcaaagac
    13441 ttctacgacg atttgaacgt caagctggta cgccctgctg gcgatatgct ggggcttcac
    13501 tctgaatttc ttcctgccag caagcgatac atcaacgatt atgtgcagta tgtcaaaagt
    13561 gattttctag ctgggctggg ctttggtgcg acacagcagt taggggaaaa cacgggtatc
    13621 tatatcggct attctgtcga tacgggacgc aatgtttatc tacaaccgtc ccttgccagt
    13681 caaggcgtaa aagatacggt tactaatgct cttgcttctg cctttgtaga aagtcttggc
    13741 ggtggaaaat cattctgtaa caacctgctt gtctactact ctgtgctgtt tggcggtcaa
    13801 gcggtaatct tagacccaaa atccgagcgt ggcaactgga aagaaacgct cccagaaatc
    13861 gcccatgaaa tcaatatcgt gaaccttacc agcgacagag aaaatgcagg gcttcttgac
    13921 ccgttcgtga ttatgcagaa tgtgaaagac gctgaaagtc tggcaatcga catactcaca
    13981 ttccttacgg gcatttcttc aagggacggc gagaagttcc ccgtacttag aaaggcagtg
    14041 cgtgccgtta cccagagcga ccagcgagga ttgctccatg tgattgagga attaagaaaa
    14101 gaagatacac ctatctcttg caatattgcc gaccatatcg acagcttcac ggactacgac
    14161 tttgcacatc tgctgttctc ggacggtacg gtgcaaaatg caatcagctt agacaaccag
    14221 ctcaatatca ttcaagtagc cgacttagtg ctacccgata aggacacgac ctttgaatta
    14281 ctgtccgttt ccatgctgat tgtgattagt acctttgcgt tagattttat ccactcggat
    14341 agaagtgttt tcaagattgt agaccttgac gaagcatggg cgttcttaaa tgtggcacaa
    14401 ggcgaaacgc tctctaacaa gctggttcgt gctggacgtg ccatgaacgc aggcgtttat
    14461 tttgttacac agtcctctgg cgacgtgtca aaggaaagtc tgaaaaacaa tatcggtttg
    14521 aaatttgcct ttagaagcac cgatattaac gaaataaaac agacccttga atttttcagc
    14581 attgacaaag aggacgagaa caaccagaag cgattgcgtg atttggaaaa cgggcaatgc
    14641 ttgcttcaag atttatacgg acgtgtgggc gtggtgcaga tacacccagt ctttgaagaa
    14701 ttactgcacg cctttgatac cagaccgccg atacagcgaa atgaggtgga gtgatgaaag
    14761 aaaggataaa aagtgtgttc acaagaaaaa agatattccg ctttctcaaa atggctctgt
    14821 ttgtcgtggc actttccctt atcctgcttt cacttttggg aacggtggca cacgcaacgg
    14881 gacttgtgga cgacactatc aacaccgaga acctttattc caaatatccg ctgtcgaatt
    14941 atcagcttga tttctatgca gataataact gggcgtggtt gccgtggaac tggctggacg
    15001 ggattggaaa aagtgtgcaa tatgggcttt actgtattac caactttgtc tggacgataa
    15061 gactatatct aagcaatgca acgggctatg tagtgcagga agcctacaag ctggacttta
    15121 tcaacgatat ggcagacagt atcggaagaa gcatacagac ccttgcaggc gtaactcaaa
    15181 atggtttttc tacttcgggc ttttatgtgg gatttcttct gctgattatc ttagtcgtag
    15241 gtatctatgt tgcctatacg ggactgataa aacgggaaac cagtaaggca ctacacgccg
    15301 ttatcaactt tgttgtggtg ttcgtattat cggcttcttt tatcgcctat gctcctgact
    15361 acatcaagaa agtgaatgac ttttcatcag acatcagtac cgcttcactg gacttgggaa
    15421 caaaaatcat gcttcccgac agcgacagca agggcaagga cagcgtggac ttgataaggg
    15481 atagcctatt ttccatacaa gtacaacagc cgtggctact gcttcaattt ggtaattcgg
    15541 gtatagagga aatcggggca gaccgtgtcg aagctctcgt atcggcaagc ccagaggacg
    15601 aggacggcaa gaccagagag gaagtcgtga aaacagaaat cgaggacaac gacaacaaca
    15661 atctgacgat actgcaggtg gtaaaccgtt taggtatggt gttcttcctc ttgttcttca
    15721 acttgggtat cacgatattt gtattcttgc ttacgggtat gatgttgttc agccagatac
    15781 tttttatcat tttcgctatg tttctgccta tcagctttct attaagtatg ataccgagct
    15841 atgagagcat ggcaaaacaa gccatagtcc gtgtgtttaa tacgattatg acaagggcag
    15901 gtattacact gattgtcacg gtagccttta gtatttccag tatgttctac aacatatcga
    15961 cggactaccc gttctttatg gtggcgttct tgcagatagt gtattttgca ggtatctaca
    16021 tgaagctggg cgatttaatg agcatgttct ctttaaacgc tggcgacagt caaagcatgg
    16081 gacggcgaat attccgcaga ccgtatctgt tcatgcgaca tagggctaga cgtatggaac
    16141 ataggcttgc aagagctgtt agtgctggtg gtgtcgctgg tgcggtggct ggtggtgtcg
    16201 ttgccagttc aaaatctaca cgccagaatg gaacaaagaa caatcgtgga aatacaagct
    16261 ccagcatggg gcagaaagca ggttcagccg tgggagctgt ccttgataca aagaataagg
    16321 tcaaggacaa ggcaactgct gtcaaagaaa atatcaaaga tatgccgaca cagactgcct
    16381 atgcggttca ctctgctaag gaaaaggcga agtccagtgt gtctgatttc aagcgtggcg
    16441 tggtgcagga aaaggaaaac cgccagtctg gacgcttaaa gaagcaacag ctacatagac
    16501 agaatatcgc tgacaagcgt atggaacttc aaaaggcaca agaatcaagg caggcaggta
    16561 agaaaacgga cggatcagcg acaagcggag ctacccgtcc ccatgaaaga ccagctacca
    16621 cttctaaggt taatgtagaa aaacagcagg aagtcaaacg ccctgccata gcttcaaccg
    16681 tgaagaatga tgaacctatc aagaaaaatg ttgtcaagga acgtccacta tcttctggcg
    16741 atacatcagc aaagaccgtc aatcaagaaa ccacaagaca gaattacaag actgaccgtg
    16801 ttacgaaaac aaccgttcag aagcatacac agcagaatac cgagaagaac aaacgaaccg
    16861 tattcaagaa aggacaaaag aagaaatgaa actaagacat atcgctattg ttggcagtct
    16921 gttttctatc ctcttttctc tcgtattatt ctttggggtt ctgattagtg ccgaggacga
    16981 cgacgggggc aatggttatt cctctaatta tgtaggtatg aacctatctg ccgaagtctt
    17041 gaaacatcag cctatggtag aaaagtatgc caaagaaaac ggtatttccg agtatgtcaa
    17101 tgtgttgctg gtaatcatac aagtggaaag tggcggtaca gccgaggacg ttatgcaaag
    17161 ctctgaaagt ctgggactac cacccaactc actagatacg gaaagctcaa tcaagcaggg
    17221 gtgtaaatat tttgcgtccc tgctttcttc ctgcaaaaat caaggtatca gtgatataaa
    17281 tgttgtgata cagtcctata actatggcgg tggctatgtt ggctatgtgg caggaaacgg
    17341 aaaaaagcat atgttcaacc ttgcggaaaa cttcgcaagg gataagtcgg gcggtcaaaa
    17401 agtaacctat acgaacccta tcgccgtggc aaaaaatggt ggctggcgtt atggctatgg
    17461 aaatatgttc tatgtggagc ttgtgaacca gtaccttgtt gtcaatcaag tatcgggcga
    17521 gctggcacaa aaggttatga atgaagcctt aaaatatcaa ggttggaagt atgtatatgg
    17581 tggcagtaac ccgaacactt cttttgattg ttcgggactg gtacagtggt gctacaacaa
    17641 agctggcgta tctttaccga gaaccgcaca aaatcagtat gacgcaaccc agcatatccc
    17701 actatcgcag gcaaaggcag gcgatttggt ctttttccac agtacctaca atgcaggaag
    17761 ctatgtaacc cacgtcggta tctatgtagg aaacactcag atgtatcatg caggcgaccc
    17821 tatcggatat gccgatttaa acaatgctta ttggcaacag tacttgattt gtgctggaag
    17881 aataaaacag tagaaaggat tggaaaccat gtttaaaaag aaagaaaaac cagtaaaaga
    17941 aacgaaagtc cgcactatga aagtaggcac acataagaaa tctgtgattg ctctgtgggt
    18001 ggtgcttatc gccagcgtat catttggcgt gtataagaac tttaccgcca ttgatatgca
    18061 cacggtacat gaaacggaaa ccattcagtt aagactaaat gacaccaacg gagttgaaaa
    18121 ttttgtgaag aacttctgta agtcctacta cacatgggac aacagcaagg aagctatcga
    18181 agcaaggacg caggcaatca gcaactatct gacaaaggag ttgcaggact tgaaccttga
    18241 taccattagg acggatatac cgacaagctc cactgttaca aatgtactga tttgggatat
    18301 aaaacagtct ggaacgaacg attttatcgc tacctacgaa gtagatcagc agataaaaga
    18361 gggagaacag acaagcaatg tcaaagcaac ctataccgtc aaagtccatg tggataaaga
    18421 cggcgatatg gttatcgtac agaacccaac cattgcacca gcgattgaaa aatcagacta
    18481 tgagccaaag gcacaagaag ccgacgcaag cgtggacgca gataccgtca atgacgctac
    18541 cgcatttttg gaaactttct ttaagctgta tccgacagca acagaaaaag aacttgccta
    18601 ctatgtggct ggcaatgtgc ttgaacctat cggcagggac tacctctatt ccgaattagt
    18661 caatcctatg tttacggatg acagggataa tgtgaaagtc agggttgcgg tgaaattcat
    18721 tgataatcag acaaaggcaa tgcaggtatc acagtttgag cttgtgttac ataaggatag
    18781 caactggaaa attattatgt aagatatttt gttggcaggc tctattttcg gatatagtct
    18841 gccttttcta tagcaaatgt gctgcactaa caagcgattt cattgtattt taagcaatta
    18901 aatctttctt gacaatttta tcttaaatca tatatttatg gatattgact ttctgcttgc
    18961 aataagttat aatatgacta taaaactaaa ttagtcatat aggaggcgtg tggtatgtat
    19021 aaagaagttg gtatttcttc aaataatgtt ccgataacac tttctatttg gtatacagaa
    19081 agaaatagtc ctaccattgt ttttttgccg ggtactatgt cacaccctct catgtatgag
    19141 aattttctat gtggtttgag tgaaagagga tttaatataa taggcattca ctatctttca
    19201 cacggaaaaa gccctaaaat caaaaaaaat tatacaatgg aagatttgct gcataatgta
    19261 tatgacgcaa cgacttacgg aatagaaaat tttggtgaaa acatagctgt catgggttcc
    19321 agtcaaggtg ggattttagc tgcaatggcg gcgggtaggg acacaaggtt aaaagccgtt
    19381 tttccacata atatcatgct gactacatta aaagaaacta tgagcttaac aaaatacccc
    19441 gcatggtcac accgttttat ggggctgata cagttgggct ttcgtgtagg tggaaaattg
    19501 ctgcctaact ataaggttcc aggagacgct tatttagact atgacagagt attttatagt
    19561 gaacaggcaa aacaagattg cttaaatgac cctatgcttt taccctatta tcctttaaga
    19621 tttgtcgcca gcttatttaa tgctgactta tcgtgcttgg agaatggggc tatacaatgt
    19681 cctgttattt taattactgc caaaggcgat ccgcttttta gatttgatta taccaacaaa
    19741 gtatttgatt taattcatgt accgaaaaag gaattactta ctttagagct taatcgtcac
    19801 atgattttta atgaggacac ggacactaca ataaacgcat tggaagctac cttgcataaa
    19861 tatcttggat agtatattga ttatataatc agtaaatgtt atacttttca tgacgataaa
    19921 aacattttag tcataagggg gttatgagtt tgccacgatt tacggaacaa gaaaaagaaa
    19981 tcataaacag taaacttttg atagagggag aaaagctgtt ttcattatac ggcttaaaaa
    20041 aagtaacagt tgatgattta gcagctgctg taaacatatc aaaaggttca ttctatgctt
    20101 tttatcccag taaagaacat ctatatgtgg aaatcaattt tcgcttacaa aaagagttat
    20161 ttacaagtat agaaacaact gttaaaaaga aaaagtataa gaaccataga gatttagcaa
    20221 aggacgttat tatgctaagt ttaacggggg tgattacttc gcctattctt tcacagattg
    20281 atttatcttt aatggactat ctgcaaagaa aaatatcgtc agatattttt gaaaatcata
    20341 tgtacagtga tattcgtata ttggagatac tggaaggttt aggggttgta tttttggttc
    20401 cgcatacggt tatcataaaa tccctgtatt ctgttttatc ctgtttggaa caattcaaag
    20461 aggacgaaga attgaatatg gtacaaaatc ttttagtgaa tggtattatt cagcaagtag
    20521 tagcagagta ataattttgt ttggttattt gacaggcaag tggccaaaca aattttttac
    20581 agtttatatg actatatatc tataaacgtc ataaagttaa aggaggaaag aataatgatt
    20641 gatgtaaaaa aactgtattt tagttatact gacaaaccgt ttgttgagaa tgtaagtttc
    20701 catgttggga gaggagaaat tttcggtttt ttaggaccct ctggtgctgg aaaatcaact
    20761 attcaaaaaa ttttaactgg gcttaataca agttataaag gcagcgtaaa agttgcaggt
    20821 acagaaataa gggagcatac aaaccggttt tatgaaaata ttggagtaga ctttgaattt
    20881 tcaacctgct acgagaaatt cacagcacga caaaatctcg cctattttgc ttctctatat
    20941 gacaagcaac ctcggtctat tgatgaatta ttgcatatgg taggcttaga aaatgatggg
    21001 gacaaaaaag ttgctgactt ttccaaaggt atgcgttctc gcttgaattt cattaaagcg
    21061 ttagtacatg accctgatat attattcctt gatgaaccta caagcggtct tgaccctaca
    21121 aataatcgtt taatgaaaga tattattctt gcggaaaaga aacgcggaaa aacgattatc
    21181 attactactc acaatatgta tgacgcaaca gagctatgtg accaagtagc ctttattgtt
    21241 gccggaaaag tcagtgcatt ggatagtcca cataatttga ttatgtcaag gggagctgcc
    21301 aaaatacact atacctacta tgataaaggc gagaaaaccg gagaatgtct gcttgataga
    21361 accacagaag ataagttgtt aaaaagttta atttccgaaa accgtctgct ttctattcat
    21421 agtagtgagc ctacattaaa tgatattttt gttgatatta cagggaggac acttcaatga
    21481 gattgggacg tttaatttgt ggtgatatac atttccaatg gaaatatggt ttctatttta
    21541 tctactttat attaacagtt ttatatgtgt gtgggattgc cgctttgacg ggacattgga
    21601 aaacggatat tgcttctatc atgatatact ctgacccggc agctatggga ttgtttttta
    21661 tgggggcgat tgtcctttta gaaaaaagcc aaaaggtatt aaatgctatg gtagtatcgc
    21721 ctgtaaaagt atcggagtat atactttcaa aaaccatcgc attgattgct atttctacaa
    21781 tcattgcggt gatattggga tttgtttccg gcagcaatca tttacttggt atcgcagttg
    21841 gtacagcttt gacatcagca atctttacta tgcttggcat tatcgcggct acaaaaattt
    21901 caaacttgaa ccaatttctc attgtgatta tgccgataga aattgtttgc tttgttccac
    21961 ctatcgttgg tctgtttgta aagttaccag atatattccg cttttttccg ttcacagctt
    22021 gcatgaacct cataactgga aaaagcattt tactatcgtt tgatatggta cttgtaattg
    22081 ctacgttgat tatcctctac atagttgcgc gacatacagt taagcatatg tggagaagtt
    22141 tgggaggtgt aaagctatga aaaggatatt atccagtacc atacaggtat ttaagcaaat
    22201 caagagcgac cctatgatgt ttgcagcttg ttttacgcct tttgtcatgg gagctttgat
    22261 taaattcggt attccctttc ttgatggaat aacagatttt tctttgcaaa catactatcc
    22321 gatctttgat ttattacttt ctatcatggc tcctgtattg ctctgctttg catttgcaat
    22381 gattacatta gaggaaatcg acgataaagt gtcacggtac ttttcgatta cccctcttgg
    22441 taagtcgggg tatctcttta caaggttggg agtaccctca attatttcgg cggtcatcgc
    22501 ttttattgta ctgttgcttt tttcattaga aaagttgtcc attggaatga caatatgttt
    22561 ggcgcttctc ggctcagtac aggcaattat tgtttcgctt atgattataa cattatcaag
    22621 caataaatta gagggtatgg cagttacaaa actagctgca cttacattac tcggaatacc
    22681 agctcccttt tttattgata gttactacca gttcgcggtt ggcttccttc catcattttg
    22741 ggtagcgaaa gccatgcaga atgaagcggt tctttatttc tccataggat tgatggtagc
    22801 tttggtctgg tactacttcc ttataaaacg tctgtttagg aagctggcag gataagaagg
    22861 catattatgg aaaaaggtag acttctgcaa aacgaaaata taaataaggc tattcaggtt
    22921 tccaaaatca cactaatcat caacctgggt ttatcgctac ttaaatttgc tgccggatat
    22981 ataggaaagt caagtgccat gctgtcggac gctgtacact cggcttctga tgtattaagc
    23041 acaatcgttg tgatggttgg aattaaaata tcagaaaaac agcctgataa agaacatcct
    23101 tatgggcatg aacgtatgga atgtgtggct tccattattc tctctgttgc attggctata
    23161 acgggtgctg gaataggata ttcaggtatc aagaaaatct tttcaggaca gtacaatacg
    23221 ctttctgtct ctagtggaat cgcacttacg gcggcagtct tatccattgt tataaaagag
    23281 tggatgtact ggtatacaag aagtgctgca aagcatacaa attctgacgc acttatggct
    23341 gacgcatggc atcaccgctc tgatgcactc tcgtcggttg gttccttaat tggcatttta
    23401 ggagcacgtt taggctatgc tattctcgac cctattgcaa gcattgtgat ttgcggttgt
    23461 atcttaaaag ctgctttgga cattttcaaa gaaagcatta acaaaatggt agaccatagt
    23521 tgtgacaatg ctactgaaac gaagattagg gaagttgtgc ttcagcagca aggggttgat
    23581 ggtattgatg aattaaaaac ccgcatgttt ggagcgaaaa tgtatgtaga tattgaaata
    23641 ttagctgatg gtaatttagc tctttatgac gctcatagaa ttgctgaggg agtccatcaa
    23701 acgattgaaa acaactttcc acaatgtaaa cattgtatgg tacatgttaa tacgaatgaa
    23761 cttccctatt ctccatagga cagggaagat gatttaagac tattgaaagg gaaaggaggg
    23821 gaagtaggtt atggaacaat actggtggat tatcattgtt atggttttga ttgtcgttgg
    23881 cttacttaaa attgtaatta tgaagaagta taaccaaagg agaaattcta acaatcgttc
    23941 aaaaaatagc gaggatgaca agatgtatta agagcaatta tccgatggta tgagatatat
    24001 acctatgaaa tttgaacgat aaatcccagt ttgtaaaagt caataaatcc aaagataaat
    24061 ttgtatttga aaggagtttt gaagaatgaa aaaatatgag tatgtagaaa ttaattataa
    24121 tgcgaaagga gttgtattcc tttgtactga taaacacaaa gaaattattg acagttatgc
    24181 aaaaacgggg tatcactacg cagggtttgt acccacagaa attgacgcaa aaggctgtat
    24241 gcgaaagata gatttaattt ttgaaaaaga agattaactt attttaggga gttggttcta
    24301 atgataggac ttaaaagacg agatatgaat tatattatga aagttggtgc taaagctcaa
    24361 ggtgtttctg tatctgaatt tagggctatg ctacaagata ccatagacaa aactatggaa
    24421 agtacagacc cagacgtgca ggcaaatttt aaacgatact ttggaaataa gcggccaaca
    24481 ccagaggaat atatttatac gattaccaaa aagacaaagg ttaaggttta aatgttgtaa
    24541 agggggcttg ccgtatgaaa cgctttaaat acaattcagt atattcaagc ccaatttaac
    24601 agcatactat ttacaattaa atacgctata ccagtagctc gtacaaaatt tatattttgt
    24661 gcgggctttt ttaatacccg aaaagttttt taattatttt catttttttg gaagcaaatc
    24721 acaccttgct gtacagatat ggtgcggaaa gagggataaa cacttttcaa ttcatagctg
    24781 gaaaggaggt gagattatga aaccgtctga cttccaaaaa acaattcagt gtcagtttga
    24841 atgtaagctg aaaaaagttg tcaaaggtat tatctggaac taccgcaaag aattaagacg
    24901 aagaaaaaac aaggaaatat cttttagcga attaccagac attacggtag aaagtctggc
    24961 aacatgggac gactacgaaa caaactacac aatttttaat gtgtgtggca tagatattaa
    25021 agtccttgat gatgaacttg cagaagctct caaacagcta cccgaaaaaa acgtaacaat
    25081 gtattgatgt attacttctt ggaaatgagt gatacagaga ttgcagatat tatgaattat
    25141 accagagagg ctgtgttcaa acacagacac cgtgcactag aaactatgaa agagatacta
    25201 aatgaaaaga caagacagta aaggacgcaa tttgcgtctt ggagagagcc agagaaaaga
    25261 cggaagatac gtttataaat ataccgatat attcgggaag ccacagtttg tatattcttg
    25321 gaagcttgta cccacagaca agacacctgc tggaaaacgt gatgatatat cgttgaggga
    25381 aaaagaaaag cagataaaaa aagaccttga cgacggtatc gacacggtgg gcggtaaaat
    25441 gacagtctgc cagctttacg aaaaaaagaa caatcaaaga aagaacatca agagaaacac
    25501 ggaattagga cgacagtatc ttgtgaacgc actggaaaat gacccattgg gtatgagggc
    25561 gattgatacg gtcaagcagt ccgacgctaa agaatgggct atcagaatga atggcaaagg
    25621 ttattcttac aagacgatag ataactataa gcgttcccta aaggcttcat tctatatggc
    25681 gatacaagac gactgtatca gaaaaaatcc ttttaattcc aagttgagtg atgttctgga
    25741 agatgatacg gaagcaaagg ttatcctgac accagagcag gaagaaaaac tccttgcctt
    25801 tatggaaaca gataatgttt acagcaagta tcgtgatgaa gttattcttc tgctggaaac
    25861 gggacttcgt atctctgaat tatgcggact gacaacacac attgatatgc ttaaccgtgt
    25921 gattaacata gaccaccagc tattgcgtga tacagaggtg ggctactata tttccaaagc
    25981 taagacaaaa aacggtaagc gagagcttcc attaacagaa agagcatatc aagcactcaa
    26041 gcgaatttta aagaaccgag gaaaagcaca accgcttgtt gttggtggtt atagcaattt
    26101 cttattcttg aaccgtgagg gattgccaaa ggtagcagga aattatgaaa gtatggtgcg
    26161 tgggttgatt aagaagtaca acaagaccca taaggacaag ttaccaaaca tcacgcccca
    26221 ttcattcaga catacttact gtacgaacat ggcaaataag ggaatgaacc caaacaccct
    26281 gcaatacatc atgggacacg caaacataac catgacatta gggtattatg ctcacggtac
    26341 tttccagtct gcaaaggcag agttagaaag gctggcttct taatatcgaa aatgtatctt
    26401 actacttatt tactactttt ggttgcattt tcatgctggt aaatgccagc ttatgcaagg
    26461 tatcttccaa aaagaaaatg cccatacagc ccgaaaatgc gggatatatc ggcatctacc
    26521 aacttatgaa aagataatca gaaatttagt aagtttttat aataaaaaaa taagaggctg
    26581 tccaaaaata gagataagcc ttttttcata atcggaatta taaaaataca cttgatttca
    26641 tatgaaaaag agtgtctcat aaactaaaaa gtttactttg agacactacc ttttttattc
    26701 ttatttatac tttgtagaca tattataata tgcgtttcac tggtatctga acttctgtca
    26761 accagtcttc ttcactctct ttattccata taccatctat ataagattct ctaggatttc
    26821 caaccacttt atatttattg tcctctatcc atttaaatgc atctgcataa gtttttccta
    26881 atgttgaata tggaccattg tgtaatatac aaactgcatt ttcaactgca tctaattctt
    26941 taaattttaa tatatccgta tcttccttta aatcatttat agcttgacac atttcaacat
    27001 ccatatcagt ctctctatat tcaccatcat gatatatatt aaaacaataa tcaggaactg
    27061 cacatgtaca ccctgttttc atcatttctt cagccattac atttggaaat agattaaaaa
    27121 gttcatcata tccctttaac acttttctca tagaagcaac tttaactttt ggtaaagact
    27181 taataattac atcattcata ttgacttctc ctttcaaatt cataaggtaa ttacgtagtt
    27241 taattatttt atattcttca cttttaatta catccaacat ttctttttct ttattcatta
    27301 aaatattttc aacttcttca ggattttcta cagctttttt tatctcagct aatgataaac
    27361 ccatttgttt taaagttatt atctgatgta atttaggtaa ttgctctgtt gtataatacc
    27421 tatattttgt aaattcatct acatattctg gttttaacaa ccctatatct tcataatacc
    27481 ttaatgtctt tgtagttatt ttatttattt tagaaaacat tcctatagaa aacatctcat
    27541 cacctgcgca ttagatttga ttaatttaag tttaaacttt ccccttaggt taaagtcaat
    27601 acaaaataaa ttataattgt attaattctt caattctatc tccactcaat accaaaatat
    27661 cccctgtttt tataatggtt gaacctttag gtattaaaac ctgttccttt ctttttatca
    27721 taactattaa tatatcgtct ggtatattag aatctattat actcttatta gcccatttat
    27781 taccctcatc aacatttact tcgataagtt tagagctaat ttccccatca taatcattaa
    27841 atgtttttaa tacaggtgta ttatcatcaa ctaaatccaa ctttctggct acagttggta
    27901 tcaatgaacc ttgtattcct actgaaagca acgctataaa aaatattata tggaatatgt
    27961 cattctctat ggctactcct tgagtaacag cataaattgc aaaaactact gaagccgcac
    28021 ctctaagacc tacccacgat atgaataatt tttctttatt tgagaattta aattttgcta
    28081 gaactataaa tgttgctaat ggtcttgcaa taactatcat gaatatagat atggccattg
    28141 ctataccaaa tacatttggt aacttagaag ggaaagataa caatcctaac ataaagaata
    28201 acattatttg cataatccat gaaattccat caaagaaatg aaccaaactt tttttaagag
    28261 gtatcctact atttcctata ataatacctg aaatgtaaac acttaaatat ccatttcctc
    28321 caagatactc gctaaatgca taagaaagta ttgcaatagc tgttataaat atagtataaa
    28381 atccttctat ctcgaagttt gaatgcctta aaatataaac agataatttt gctagtataa
    28441 atcctatagc taacccaaaa gctatttgtt ttattaccat tggtgcaata agttctaccc
    28501 cagaattatt catcaatgtc aaaagaatta gtgtcaacat atacgctatt gggtcattac
    28561 ttccactttc aacttctaat aatgatgcaa gtgaacccct taagtttagt ttttgtgaac
    28621 gcaacacagc aaacacagat gcagcatctg ttgaagctgc aattgcccca attaataaac
    28681 cttcaagtaa actagttcct attaccatgt ggcaaaacat acctgtaacc ccagacgtaa
    28741 ttattacacc cagtgaagac attaatatag aaggtatagc aacaggttta gccatattcc
    28801 agtttgttcc aaaccctcca aaaaacatta taaatattag tccaatagaa cataattgtt
    28861 ttgttaattc atagttctga acctcccatg actaaagtca cagagttcct gcttcataga
    28921 attttgcata ctaaaatatg tcttactaat tctccacagg ctatcccc
//
LOCUS       DAEPLF010000026        28628 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEPLF010000026 DAEPLF010000000
VERSION     DAEPLF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.026"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            155..991
                     /locus_tag="KR314_001052"
     CDS             155..991
                     /locus_tag="KR314_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0327450.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            1012..1434
                     /locus_tag="KR314_001053"
     CDS             1012..1434
                     /locus_tag="KR314_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBH0327451.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(1645..2808)
                     /locus_tag="KR314_001054"
     CDS             complement(1645..2808)
                     /locus_tag="KR314_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH0327452.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(2862..3062)
                     /locus_tag="KR314_001055"
     CDS             complement(2862..3062)
                     /locus_tag="KR314_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBH0327453.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            complement(3268..4944)
                     /locus_tag="KR314_001056"
     CDS             complement(3268..4944)
                     /locus_tag="KR314_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBH0327454.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            5161..5898
                     /locus_tag="KR314_001057"
     CDS             5161..5898
                     /locus_tag="KR314_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBH0327455.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            complement(6015..6473)
                     /locus_tag="KR314_001058"
     CDS             complement(6015..6473)
                     /locus_tag="KR314_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBH0327456.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(6755..7042)
                     /locus_tag="KR314_001059"
     CDS             complement(6755..7042)
                     /locus_tag="KR314_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBH0327457.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            complement(7120..7545)
                     /gene="ruvX"
                     /locus_tag="KR314_001060"
     CDS             complement(7120..7545)
                     /gene="ruvX"
                     /locus_tag="KR314_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBH0327458.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            complement(7751..9922)
                     /locus_tag="KR314_001061"
     CDS             complement(7751..9922)
                     /locus_tag="KR314_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327459.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYGTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNVGYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIININMIIFEKNN"
     gene            complement(9932..10189)
                     /locus_tag="KR314_001062"
     CDS             complement(9932..10189)
                     /locus_tag="KR314_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBH0327460.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            complement(10214..12853)
                     /gene="alaS"
                     /locus_tag="KR314_001063"
     CDS             complement(10214..12853)
                     /gene="alaS"
                     /locus_tag="KR314_001063"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBH0327461.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     misc_binding    complement(12921..13159)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(13302..14381)
                     /gene="mnmA"
                     /locus_tag="KR314_001064"
     CDS             complement(13302..14381)
                     /gene="mnmA"
                     /locus_tag="KR314_001064"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBH0327462.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     gene            complement(14670..15110)
                     /gene="nifU"
                     /locus_tag="KR314_001065"
     CDS             complement(14670..15110)
                     /gene="nifU"
                     /locus_tag="KR314_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBH0327463.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            complement(15164..16357)
                     /gene="nifS"
                     /locus_tag="KR314_001066"
     CDS             complement(15164..16357)
                     /gene="nifS"
                     /locus_tag="KR314_001066"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBH0327464.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGRVDLRQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            complement(16375..16821)
                     /locus_tag="KR314_001067"
     CDS             complement(16375..16821)
                     /locus_tag="KR314_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBH0327465.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            complement(16930..17700)
                     /locus_tag="KR314_001068"
     CDS             complement(16930..17700)
                     /locus_tag="KR314_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0327466.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDTLAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            complement(17731..18996)
                     /locus_tag="KR314_001069"
     CDS             complement(17731..18996)
                     /locus_tag="KR314_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBH0327467.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            complement(19103..19888)
                     /gene="codY"
                     /locus_tag="KR314_001070"
     CDS             complement(19103..19888)
                     /gene="codY"
                     /locus_tag="KR314_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBH0327468.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            complement(20053..22140)
                     /gene="topA"
                     /locus_tag="KR314_001071"
     CDS             complement(20053..22140)
                     /gene="topA"
                     /locus_tag="KR314_001071"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBH0327469.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            complement(22173..23273)
                     /gene="dprA"
                     /locus_tag="KR314_001072"
     CDS             complement(22173..23273)
                     /gene="dprA"
                     /locus_tag="KR314_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBH0327470.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGYAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            complement(23292..24893)
                     /locus_tag="KR314_001073"
     CDS             complement(23292..24893)
                     /locus_tag="KR314_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBH0327471.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLLVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVSIMSFLDDKSRKENGELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISIEEMIEVSKV
                     YSILGMINESKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            complement(24924..25268)
                     /locus_tag="KR314_001074"
     CDS             complement(24924..25268)
                     /locus_tag="KR314_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBH0327472.1"
                     /translation="MNNKEKGDFGEEIAVNYLLSKGAKILEKNYRLRIGEVDIIANIG
                     DEVVFVEVKSRSNIKYGYPCESVDFKKRKKIIGVAKYYIIKNNLNNTPIRFDIIEVYL
                     LEKRINHIMNAF"
     gene            25453..26613
                     /locus_tag="KR314_001075"
     CDS             25453..26613
                     /locus_tag="KR314_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0327473.1"
                     /translation="MNRKKTFIFIRYLSLFILLISYLNHTENTLYPAITMIILFLIIN
                     NQVRFFSLSSENKFILISYFLELILIFIFCKYIKTFNPLYYVSLILDASFLIKEKYKY
                     ILFSLVIIFSFIIGLDKNLYLALESSSTLLIITLFSVYLENENLSKLYNQSLYDKLRV
                     SKDELKKANADLEIYANSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLLK
                     DKPLISDLVHELREFVTESFQDVRRAISELKPIEYENYQNLLKIRELVNSFIKLTNIN
                     VRLTISKNTWNLSTNQSIALYRLVQESLSNASRHGKATEIRIFITFNPSNLIITISDN
                     GIGCNNIKRGNGLSSISERIYELNGKVEFDNSNTGFTIRASFPKFSGGDFFE"
     gene            26606..27250
                     /locus_tag="KR314_001076"
     CDS             26606..27250
                     /locus_tag="KR314_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0327474.1"
                     /translation="MNKIKVLIVDDEKLIRKGLNIILSSYDDLEIVGDASNGYEALEF
                     CKTNDVDIVLMDIRMKVCDGVLGTRLIKEYDSSIALLILTTFNDDEYIEDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMYPQKTIEQKHISDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILGKLELRDRTQLVIFAFKNKIVI"
     gene            27438..28373
                     /locus_tag="KR314_001077"
     CDS             27438..28373
                     /locus_tag="KR314_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327475.1"
                     /translation="MDIVRVNNITKRFNDKLALDNISFSVKEGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGDIQINGLDLSKDCIKAKSIIGLVPQELALIETLTPFDNLEYFGT
                     YYGLKGKLLQERITEALEVTGLTDVKKKKIKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKNISKKQKTTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVVSILENNSLLELGVDKKLQLTDV
                     LSVVDKNNSKITRISYEEPSLEDVFLNLTGKNLRD"
     gene            28387..>28628
                     /locus_tag="KR314_001078"
     CDS             28387..>28628
                     /locus_tag="KR314_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0327476.1"
                     /translation="MKLYISVKTMLKVLKSSFMLNLIYFLALPLVLAGFLGMVTESIF
                     QNPIKTDLTSIVIYDKDNTKLSSNLTGYLKNDLSSI"
BASE COUNT         9093 a         5034 c         3162 g        11339 t
ORIGIN      
        1 atcatattca ttgtgaaaat ataataatta cacctactgt aagtgtaata ttatattgac
       61 atgaattgac attattgtct cttagtcaat attatacaag aagtataaaa aaatatgtgt
      121 tacactttgt ataacatgaa aggaggttac ctaagtgaga caagaagaac gcacagtatg
      181 ctttgatagg gaattaaaaa ttgaagcata tagatttaaa ggcataatgc aaaaattccc
      241 aaaccatttt catgaacatt atgtaattgg ttttatagaa aaaggtcaaa gatatttatc
      301 ttgtaaaaat aaagagtata ccacaagtac tggagatttg ctacttttta acccttttga
      361 cagtcatact tgtgaacaaa tagatgataa agtacttgat tacagatgta tcaatattaa
      421 gccagaaata atgaaaaaaa ccgtctttga aattacaggt aaaaattatt taccaaaatt
      481 taatcaacct gttatatttc gcagtgagct agtacctcta ctacaagaat tacactatat
      541 aatcatggaa gaagaattgg attttaaaaa ggaagagctt tttttcttcc taattgagca
      601 attaattgaa gaacatacag agccaaattt gcaatctaac ttggaaaata caaatataga
      661 aattcaagca gtatgtgatt atctagaaaa taattatgct gaacatatag tacttgacga
      721 attaagcact atagcaggta tgaataagta ctctctactt cgtaatttta cgaaattaaa
      781 aggtattaca ccatatagat acctagaaaa tatacgggta aacaaagcta aaaagctttt
      841 agaaaaaggc gtagaaccta tagatgctgc gatacaaaca ggatttgtag accaaagtca
      901 ctttacaaac ttttttaaga attttattgg tcttacacca aagcagtatc aaaatatatt
      961 tataaatgat gattcaaaca actttaaata aggtaaaaag gagaaataaa aatggaaaat
     1021 aaaaattcaa aatgtgtcat ggtaatagac gaaaatttac caatgggaat tatatccaat
     1081 acagcagcta taatgggaat tacactagga aaacatgccc cagaaactgt tgggccagat
     1141 gtaattgaca aaacaggtaa tagccactta ggaattattg acattcctgt acctatttta
     1201 aaaggaaaca aagaaataat aaaagattta cgtaaaaaat tatatacttt ggaatttaac
     1261 gatttaactg ttgtagattt ttcagatgta gcacaaagtt gcaatcttta tgaagaattt
     1321 acacaaaaga ttgctagtgt tccagaagat gagttacagt attttgggat agcaatttat
     1381 gggaataaaa agaaagtgaa taaacttact ggtagtatgc ctttacttag ataattgtta
     1441 aacaacccta tatatgatat taaatcaaat gatattttaa gatacataaa cttttctata
     1501 tttctataaa aatcataaga ttataaaata cttctatatg gtaaataaaa atacccctat
     1561 ggcaaataac tacataaggg tattttttat gtgtattata atcatatctt tgtgtattat
     1621 aattatattt catctgataa ttatttatat catattatca attattcttt gaagcatttg
     1681 tagatagctt gccttattta tatccttagt atttatcaat tccacttcgc ctattacttt
     1741 tttatcttta catatcttta tttttcctaa aacagttccc tttttaattg gcatcttagg
     1801 attttttact atttcaattt tcttctcaaa ttctttagag cttccttttt taataagagc
     1861 atttaaatct tctttagcaa ccaatttaac tttgttttca tcagccttat ccaatgtaag
     1921 tgttgctata ttatcacctt tactacataa cttaacactt tcataattag caaatccata
     1981 gtttaaaagg ctagaggcat cattaaatct ttctggagaa gtctctgctc ctaaagttgc
     2041 agcaactaga tgagtattac ctctctttgc agatgctgat aaacagtatt ttgcttcttg
     2101 agtaaaacca gttttaacac cagttgctcc ttgataatgc tttataagtt tattagtatt
     2161 tgcaagacct actgtaactt gtttcttccc tactacaacc ttatccatcc atgtagttaa
     2221 gtatttactt atcatttcgt gttttaatag ttctctagac attaaagcta tatcatgagc
     2281 agatgtatag tggtttgcaa ctggaagtcc atttgtattt gcaaaatgag tatcattcat
     2341 atttaactct tttgctttag cattcattct atcaacaaat ccttcaacac ttccagctaa
     2401 gtgttcagcc atagcaacac aagcatcatt tgcagaagca acagctatac cttttaataa
     2461 tgtatcaacc ttttgagttt ctcctggctc taggaatatt tgactaccac ccatactaga
     2521 tgcagtttca cttacttgaa cttcatcatc aagcttaatc ttgcctgaat ccaaagcttc
     2581 tactataagt agcatagtca ttaccttagt tacactagca ggaggaagct ttttatgagc
     2641 atccttttca tataaaattt gtccactacc aacatccatc aaaattgctg attttgaaga
     2701 tatatttagt tggttggcat cagcattttc cttattattt gcaaaagaaa agcttaaagg
     2761 agtaagcgca attaatatag ccaaagcagt actagctaat tttttcatcg atgtcacctc
     2821 ttaaagttta tttaaactta ttttctcaca tttaactttt tttattctaa atgcctagat
     2881 ataccttgac ctaaattttt tttcttttca agctttttaa agtaaccttc taaaacacct
     2941 ttattagacg cattttctat tatttcaaaa gcatcttcat aattcattcc atactctcct
     3001 gctatttcca tttttgtttg ttttaaagct tttatagcat tttgtcttga aacatcatcc
     3061 ataagaatcc tcctatcagt aaaatttata tataagtagt ttttactaat aaaagattgt
     3121 tataattggt attttatgta atatatattt tttttaaatg agtattaaaa agacctcttg
     3181 taattcttaa gataatatca attggatttt tgatattact ttaaaaatta caagaggtct
     3241 aattttcaaa tttatagttt ttttatatta tacttccatt atgataggaa gtatcattgg
     3301 atttcgttta gttttttgat ataaatattc ttttaggttt tctttaatat tattctttaa
     3361 ataagcccat tctttaatat ttttctcttc acattcattt agaacattct taattatatc
     3421 ctttgctcca tccattaaat cttctgactc tcttacatat acgaatcctc tggaaattat
     3481 atctggacct gctaagacct taccctcatc cttggaaata gtaactacaa ctatcataag
     3541 accatcttct gataaatgct ttctatccct taaaactata ttacctacat cgcctactcc
     3601 tagaccatca actaatatgt ttcctgtttg tatacggttt gctaccttag cagagttttt
     3661 atctaattct aaaacatctc ctgtttgcat gacaaaaata ttttctttat ccataccaag
     3721 ctgttcagct atttcagcat gtctttttaa cattctatat tcaccatgag ctggcataaa
     3781 aaactttggt ctaactaatg catgtataag ttttaattct tcttgacagg catgtccaga
     3841 aacgtgtata tcagctatat cactgtatac tacttctgct cctttttcaa ataagaaatt
     3901 gattactctt gatattaatt tttcatttcc tggtattgga tgagcagata ttataactaa
     3961 atcgccactc tttatttcta cttttttgtg ctctgaaaaa gccattctag caagagctga
     4021 cataggttca ccttgtgaac cagttgttat tatcactaat tcactatctt catacttaca
     4081 tatatcattt aaatctataa gcatgtcatc agaaatatct aggtatccta attcttttgc
     4141 aactccgact acattgacca ttgagcgacc tgatatagca acttttctat taaacttttc
     4201 agcagtattt attatttgtt gtaatctgtg tatgttggat gcaaaggtag caactataat
     4261 tcttctgcca tttccttttg caaataattc gtcaagacct accccaacag tcttttcaga
     4321 catagtaaat cctggttttt ctgcattagt actatctgcc agcattaaaa gaactccttt
     4381 tttgctcaac tcacagattc tatgcatatc cataacatct ccatcaatag gagtcaagtc
     4441 aatcttaaaa tctcctgtgt ggtatattat accttggtct gtgtgtattg caatagagta
     4501 agcatctggt atactatggt tatttttcaa aaactctact tccatattat caagctttat
     4561 aacttgtcta ggacctataa catttaactt aacattattc attttatgtt ctttaagttt
     4621 tacttgtata agacctatac taagtcttgc cccatataca ggtacattta ttttctttaa
     4681 tatatatgga agagctccta tatggtcttc atgtccatgt gtaataaata taccctttat
     4741 tttatcccta ttttttacta aataagttat atctggtata actatatcta ctcctaacat
     4801 ttcatcctct ggaaaactaa gtccagcatc tataacaatt atttcatctt tatactcaac
     4861 aacagtcata ttttttccaa cttcgttaag tccacccaag gccattacct ttatcttgtt
     4921 ggtgttcttt ttgaacaatt gcatcatctc cttctgtcta tttaattttt taacacgttc
     4981 tacaatcata aataatatca aatcacgcac tcgctaccag ttattcttat tgtaccataa
     5041 ttcgtatcat attttaagct atatttaaaa tacatttaac gtattatttt gacaaaccta
     5101 ttttattttg atatcctcta aatattatat gatttaatat cggataattt aggaggattt
     5161 atgcgtggta aaactcattg tgcaatagga atcttaacag ctatccaaac atctttaata
     5221 tttaaaattc ctatatcttt agttgatatt ttagtttcag ctacttttgc tgtactaccc
     5281 gatttggata aatctaattc tatggtctca aatttcattc taaaaaacaa tgtatccaaa
     5341 tacatatata gaatttttat atatgctgtt aatattatta ttttctttat ttcaataaat
     5401 ataaatgata atttttattt aagcgcaata attacatttg ttgcaattat aattattgaa
     5461 gcaaaactaa cccacacatt tctaagaaaa gtttttctat cattaatatt tatactatta
     5521 gctatatgct tatacatcat agaagttgaa atatatttta caattttttg tctaatgttg
     5581 tcaatttttc cctggttaaa gcacagaagt ttttctcata gcatatttgc aactataata
     5641 gtatattttt tactaaaaca gatagaatta atcactaata ttaataattt atctttttat
     5701 ggtacgattg gttatgcaag tcatatgttt ttaggagatt tatttacaaa gcaaggaata
     5761 ccaatttttt atccgctaag cgaaaagaaa atttctttag gttttttaac tgttggagga
     5821 ccatttagta atttcataga aaaatcattc atttttgtac ttataggatt aattatattt
     5881 tcaatattaa aactataagc atcttgaaaa ctgtatattt gcattttgta gatggtttat
     5941 aataaaaaat ggaagaatag catagaatat aaaagttcta tactattctt ccatcgacat
     6001 taagcaatag aagtttattc ttctgaatcg cttactccat tacactcgtc acataatcca
     6061 taaaatttaa catcatgatc aagtatttta aatttgtaca atgattgtat ctctttttct
     6121 atattgtcta aaaggtcttc tttagcttcc attatctttc cacaattttt acaaataaga
     6181 tgatgatgat tatgacagtc ttctttatgt agactaatct catatcttat acatccatcg
     6241 tctaagttta gttttgatat taatcctatt tcatccaata gctgcattgt tctatataca
     6301 gtagctaagc ctatttcagg acagtcaacc ctaactaaat catagatttc ctcactactt
     6361 agatgtgaat ggtcatgttt aagcaatatt tcaactattg ctcttctttg tggcgtaatc
     6421 ttaaaaccag tttcttttaa cttgtctttt aataaatcca ttgtatttgc cataacatca
     6481 cccttactat tttcaattga taatctttat taaataatat tgtaattgat aattcatttt
     6541 ttgtcaagac atttaaataa atttaattta ttctcataat ataaatatat acccataaaa
     6601 gatatattat tcaccaaaat tcataaattt tttggtattt ttaataaaaa acaaaaaaac
     6661 agatatatac tataaatgct tttacaatta ataaggttta attaaagcaa atataataaa
     6721 tatctgattt ttatatacaa acttaattac ttatctagtt aagtccattt gattcatctt
     6781 cgtcgtaatt taaaccttct tcttccatta tagcagtata cacagctaca acagtttcat
     6841 actcttcatc actttccaaa ggtattaata tttcaccttc ttcatcctca tcaattctaa
     6901 agattaaagc ttcttcagct tcttcatcat ctaaaggcat taatattgca tactcttttc
     6961 cttctgcttc taatgttaat attatttcaa attggctttc aacgccattt tcgtctatta
     7021 aatttattat attttcttcc ataatgtcat tcacctcaaa tttattttta tttttattat
     7081 ttatattata tatcataagt attcaatttt atagttcttt tatgaattta ttttaaaatc
     7141 taaatatcct tgcaaaatta ttactgctgc caacatatct ataacctttt ttcttttttg
     7201 tctactaaca tctgcttcta ataaagacct ctctgccgct actgtagaaa gcctctcatc
     7261 ccaaaactct acatcaatac ctgtttctgc ttttaccaat tcacaaaact tgattacttt
     7321 ttctccttgt ggacctaaag ttccattcat attttttggt aatcctgata caattttatt
     7381 tacttgtttt tcttttatta ttgcttttaa ctcttcaata tcttttttct tgccaactct
     7441 ttttattgtt tttacacctt gtgcagttaa tcccatcaaa tcactcacag caactccaat
     7501 agttttatca ccaacatcaa gacccattat tcttccatct aacatagttc ctcctttatc
     7561 tatttatatt ctatagatta aatacatatt ctgaacctct cataactaag gtcacaggct
     7621 tcctgcttca tagaattttg cataatcaat cataaaaatt gcaatatgat tttttatgta
     7681 agcatccagt ttacattaat aatcatattg cattttttat ttaaatccta tatgaacagt
     7741 aaaaaatttt ttagttgttt ttttcaaata ttatcatatt tatatttatt attattagtg
     7801 gtataagtat atatatagat gctgtagatg cagcaactat tccagaatca ttaactaaaa
     7861 gagtaattat acaaccaacc atagatgcaa taaacccttt aaacaagatt ggaaaattat
     7921 tcattatctt tctaaagtgt ctagatggtt taaatataag tactgcaatt actccaattc
     7981 ctacaagtaa aatatttacc catacacttg tctgtgctag ttgcatattc atttgaattt
     8041 ttcttccaaa tgtttgtaat atctctcctg gtccatttag gaaaattctc tgtacaaaca
     8101 tacctaaatg agattcacta cctgaaagta tatctaaagc tgcaaatgca aaaacgacaa
     8161 ctacagctga aacagctaat aatacagctt tcttaaaatc tattttaatg tcatatataa
     8221 gcattataaa caacaaatat gctacacatt ctgatatagc tccaccaaca tttgcaccca
     8281 ttgaaggata tgcactagtt ataagtatta ttaacgaaaa aattacaaca agtaatttag
     8341 ataccttctt ataatgtaat aatactgcta agccaaatac tgcacttgct atagttacac
     8401 cttcatattc atttcctaca ccatagtacc tagctcctat gacagcatca taactcatta
     8461 tgttattttg cattaagtat gttccaaata cagaatctat aactatgact aaaatagtaa
     8521 gtgttgcaaa aaatcccatg tgctttatat cattgtcctt aaataatttt ctacctaaca
     8581 agtataatat tagcgtagtc gcaaatattc ccaaagatat tgaaattgga gttttaaaat
     8641 tcatcaaagg agctaacata aatgatagtg gcattattat gcctaatttt ataaactcct
     8701 taagtacttt gaatacacgc tctttatgtg gcatataatt tctaaagaaa atcgcaaaca
     8761 tggcaattac ccaagaagca gatattacgc ctacaaagcc atttataata gttgacctta
     8821 ttgaagatat agacactatc ctttcataaa gtgattttag atagccaaca ttgtcctctt
     8881 tatttactaa agtataactt ctacctacca tctctttatt ttcaagacca aatgtaccta
     8941 gtatatctac tccaacatct aaattggcaa caattccagc ctttcttgtt gtggcagatt
     9001 ccagtagacc tttcccttct ccactaaatt ttattattgg agaaagtctt ctcttattct
     9061 tataatctag gtcacttgga aatgcacttg ctatataaac agtgtcattt tctccaacca
     9121 tagaaaatac ttgttcaaga tatttattta tattttttac aatatttttc ttcattgtac
     9181 cataagtatt atcatttaga tttaatctat acatatctaa tctatatgta tctccaagtt
     9241 ctacaaatat agcatcacta tttttatagg attttttagt ttcatctagt aatttatcgt
     9301 agtctgtaga tattccaaat ggcattttag aatctttctt attaatatta tcaatattac
     9361 ctgaatcaat ccttccatat tggtccatag ctactaaaca taagtttcta tttttaacta
     9421 attgattatt ttcaattata tcagcatttc caattacaga tgtctttaaa ccattttctg
     9481 ataaagtttt tcctaatgac cctaaatttg ctccatattc tccatactca agattttcat
     9541 ttatagatct attaatgcct aaatcattta tttctttagc tttttgacct gttgcgactt
     9601 caaaagcttt agcagtatct tggtctactt tttgaaaatt gatatagtct tcagtagtta
     9661 cactagctct tgttcctgca cccatacttg cataagacct tctatcatca gtaccttgat
     9721 cccctcttat attcatgaga gctacatagc ctctcttttc tatctcattt ttcaagattg
     9781 gaatattcat catacttcca agattggtcc tattcatatc aataaatatg accttgcctt
     9841 tttcttcagc aaaaccatag ctagcagttg atacaaaaat cataatgact attgcaaaca
     9901 acgacataat cttttttctc atttatttga cctactttct attcagatag tgcgtgatta
     9961 cttcttcaag tatctcatct ctttcaaatt ttttaattat agctcttgca tttttatgac
    10021 tagttatata gctagaatca ccagaaagga gataccctat aatttgattt atagggttat
    10081 atcctttttc aactaatgct ttatatacaa aatcaatagt atctcctaca ctcatcctat
    10141 cttctggtat tccttcaaat ttcatagtgt agtctaaatc tttttccaaa ctaaaaacct
    10201 ccttgagtga atgttattta acttgtgtct taataacctc acttgcatag cttaaagctt
    10261 ggtcaacctt agatacatca ctagctcctg cttgtgccat attaggtctt cctccacctt
    10321 ttcctccagc tatttttgca acttctctaa caatattacc tgaatgtatt cccttatcta
    10381 atacatcttt agtggcagta actacaaagt taactttatc atctactaca tttgcaagta
    10441 ctactacacc agaaccaagc ttatctctta gattatctgc tgtttctctt agtgtatcca
    10501 tatccatacc ttcaaattta ttagttatca aattaactcc atttacttca actttagaat
    10561 caaaaataga atcaactgat tgtaaactca tcttagcttt catatcatgc aattctttag
    10621 ataagttttt attttcttct aataaagaac ttattctttg aattaaatta tcttctttag
    10681 attttaagtt tacacaaact tcagagataa ttccatctct ttcttttagg tattcataaa
    10741 cagcttttcc tgttattgct tctattcttc ttacacctgc tgctactcca ccttcagata
    10801 atattttgaa cattcctact tgagaagtat ttgtcaagtg agttcctcca caaagttctg
    10861 tagaataatc tcccatagaa actactctta cctcatcacc atatttttca ccaaatagtg
    10921 ctgtagctcc tttttcttta gcttccttta tattcataat atcacatttt atgtctaaag
    10981 atgataaaat aacattattt actttttctt ctattacttt taattcttca tttgatatcg
    11041 cttcaaagtg agtaatatca aatctaagtc tttctggagt tactaaagaa ccagcttgat
    11101 taacatgttc tcctaatact tctcttaatg ccttatgaag taaatgtgta gcactgtgat
    11161 ttcttgcagc tgacattcta gtttctctat ctacgagtgt ctttactttg tccccattac
    11221 ttattcttcc tgattttatt atacctatat gttttatact attattagca ccttttttag
    11281 tatttaatac ttctaaaact aagttttcat tagttatcaa tccacaatct ccaacttgtc
    11341 ctccaccttc aggatagaaa gtagtgttat ctagtactat agatacctta tcgccttctt
    11401 ctgcactttg aactagctca tcatctttaa ctatagcttc aatagttcct tcaccataaa
    11461 tttcactata cccattaaat gtactgtcaa ctgtagattc taattttgat agagggtctt
    11521 ccttccaact ttctccatcc atatttcctc tagcatttct agctctttct ttttgctttt
    11581 ccatttcttc attaaaagct tcttcatcta cagatagatg ttcttcttct aatatttctt
    11641 tagttaagtc tatagggaag ccataagtat catatagttt aaaagcttct tgaccactta
    11701 ggacagtctt tccttcattt tttaatactt ctatataaga gttcaatatt tctgttcctt
    11761 ggtctatagt ttcattgaat ttttcttctt ctattccaac tactttttta atataacttt
    11821 ctttttcaac taaaactgga tatgctttat catttacctt tataacttca tctattagtt
    11881 tctgtaaaaa taattctttt actcctaata acttaccatg acgagcagca cgtctaagga
    11941 gtcttctaag aacatatcct cttccttcat ttgatggaag cactccatca ctaactaaga
    12001 atgttacagc tcttatgtgg tcagttatta ttcttattga tatatcattt tttggattct
    12061 ctccatattt tactcctgtt aacttctcaa ctgcttctag tatagattta attgtatcaa
    12121 cttcaaagat agtgtctacg ccttgcataa tacatcccat tctttcaaga cccatacctg
    12181 tatctatatt cttgttttct aatagactat aatttccttc ttcatctcta tcaaattgag
    12241 taaatacatg gttccaaaat tctaaatacc tatcacaatc acagcctggt ttacagtctg
    12301 gattgtcaca tccatattct tctcctctat caaaatatat ttctgaacaa ggaccacaag
    12361 gacctgtacc tatttcccag aaattatcat cttttccaag tctaaccatt ctttcttctg
    12421 ggaaattcat ttcttttgcc catatatcat aagaatcatc atcttcttca tatacagtca
    12481 cccatatctt atcttctggt atatttagcc attctgtaac aaattcccat ccccatttga
    12541 ttgactctct tttaaagtaa tctccaaatg agaagttacc catcatttca aagaatgtag
    12601 catgtcttgc agttatacct acattttcta tatctcctgt tcttatacat ttttgagatg
    12661 tacacattct cacacttgga ggtacttcta cacctgaaaa gtagtttttc aaaggtgcca
    12721 tacctgagtt tataagtaac aaactcttat cattgtttgg tactaaagaa taactatttg
    12781 ctacataatg accttttgac tcaaaaaatt ctaaaaactt acttcttatt tcatttaatc
    12841 ccatcttttc catatcatgt tcctcctaat aaattttatt ttttcgttta aataaaaaac
    12901 ctcgtcccta taaataaatt tataggggcg aggacaatcg cggtaccacc ctaattatca
    12961 tacaaagtat gatatcttcg gaaaacctta gctataacgt aactaccgtc aatctctact
    13021 taaatcttct ttataaaaga taaattgttc aagattgcta ctaaaaggct gcttcaaata
    13081 tcatattcta agggcttcca gcatttccct ctctctgtaa gacatcaaat attttactat
    13141 tccttatcac tgtatttgtt ttttaattaa tatactaatt atattactaa attttacaaa
    13201 attaatcaat actatatttt aatatacaat aaatttattt tataaacttt atttactctt
    13261 aatatacttt attgcttatt tttttaattt tttagatgtt tttataaact tttctcgatt
    13321 atacctccac ctactacaat atctccatca tacattacta cagattgacc tttagtaata
    13381 gctctttgag cttcatcaaa aactatttta atagtatctt ctcctactct atgaatagta
    13441 gctggtgatg gttttgctga atatcttatt ttggcttgaa ctcttaaggg ttcttctaac
    13501 gtatctattg atataaagtt aacatcttta gcaataagtt cctttttaaa taaatcttca
    13561 ttgtctccaa gaactatggt attatttatt ggatttatgt ctattacaaa cattggtttt
    13621 ccaaaagtaa ttccaagccc ttttctttgg cctatagtat aatataatat acccttatgt
    13681 tttccaagta tattgccttt agtatctaca aaaaaacctt cttcaatacg ctttttagaa
    13741 tgttttttta cataatttgc atagtcatta tctttaacaa aacaaatttc ttgactatca
    13801 ggcttattgt gtactgccat ccccatttcc ttagcaattt ctcttactct atctttttca
    13861 tagtcaccta ttggcaataa agtatgctct agttgctctt gtgtaagatt ataaagtgca
    13921 taagtttggt cttttttatc tgtaacagat ttttttagca aatatcttcc tgtactttca
    13981 tccttttcta tctttgcata gtgacctgta gctacataat cacaacctat ttgtctagcc
    14041 ttcttataaa aatcatcaaa ttttatatgt ttattacaag ctatacaggg attaggagtt
    14101 tttccttcca aatattcatc tataaaataa tctattactt tttctttaaa cacttctcta
    14161 aaatttaaaa cataaaatgg tatacctatt ttatttgcta cccttctagc atcttcaaca
    14221 gcagataaag agcaacaacc accttcaatt tcaacaacat catcatcttg ccacaacttc
    14281 atagtaacac caatgacatc atatccttgt tgttttaaaa gatatgcagc tacagagcta
    14341 tcaactcctc cactcatacc tatcattact tttttattca taaacaaatt tcacctcatc
    14401 ttttgcatta cttattatta taccatatag agcaagtatt gtacttattt aaaatatagc
    14461 ttgttctatt ttctaaaaaa ataatcatct ttattatttg aatcttatgg attattttgt
    14521 taactaatta acctctaaat caagttaaaa tacttaattt aggcaataaa aaatagccat
    14581 tttaaaaaca gctatttttt atgtcctaat tatatcatta gtacttcttt ttaatcattg
    14641 taaacttctc ataacacaat cacttatttt tattcttctt cttcaacatc atggtcatga
    14701 gcatcatcta taacatatcc atccaattct ggtatatgga tattgttctt ttgagcataa
    14761 tctataagag ctgcttttac agcttgttca gctaaaactg aacaatgcat ctttactggt
    14821 ggtaaaccat ccaaagcttc tgcaacagct ttatttgtaa gttctaatgc atcttttata
    14881 gtttttcctt ttatcatttc agttgccata gaagaacttg ctatagctga accacagcca
    14941 aatgttttaa acttaacatc ctttattaca tctccatcta tgtctaaaaa tatcttcatt
    15001 atatctccac atgtagggtt tccaacttca cctacaccac ttgcattatc aatttctccc
    15061 atatttcttg gattcataaa atgttccata actttatcac tatattgcat tatcttttcc
    15121 tcctactcaa tttatgtcta ttttttatgc tttatctttc tgtctatttt actaaacctt
    15181 gtttcttcgc atcatcataa agtggtgaca tgcttctaag tctttctatt acttttggta
    15241 agttctctag tatataatct atatcatcat ctgttgtaaa atctccaact gtaagtctta
    15301 atgaaccatg tgctatttca tgtggtaaac ctattgcaag aagtacatgt gaagggtcta
    15361 atgaaccaga agtacatgca gaaccacttg aaccagcaat tcctaacata tctaataata
    15421 aaagtattcc ttcaccttct ataaattgga atgcaaagtt tgcatttcct ggcaatctat
    15481 tttctaagct accatttact cttgtgtaag gtattctttc taacacacca tctattaact
    15541 tatttctaag acgagttaaa gtttctacat gattttgcat attttcctta gctaattctg
    15601 ctgcttttcc atatccaact attgcaggta tattttctgt accagctctt ttgctttttt
    15661 cttgtgctcc accatggaca aagttatgaa gtcttacacc ttttcttatg tataaagctc
    15721 ctacaccctt tggtccatat attttatgtg aagacatact catcaaatct attcctaatt
    15781 ctttaacatc tactggaata ttacctgtag cttgtactgc atcagtgtgg aataatatct
    15841 tatgtttttt agctatttcg gatatttctt taataggctg tatagttcct atttcattat
    15901 ttgcaaacat tatgcttata agtatagtag tatcttttat tgaatcttct agttgtctta
    15961 agtctactct tccttcgcta tcaacatcta aataagttat ttcaaatcca tgatgttttg
    16021 ctaagtattc acatgtatga agtattgcat ggtgctctat ttttgaagtt attatatgat
    16081 tacctttgtt tttattagca taagctacac cctctagtgt ccagttatca ctttctgaac
    16141 cacctgctgt aaaatatatt tcactaggtt cagcatttat aagggctgct acttgttctc
    16201 ttgctttatc caaagcatct tttgcttctc ttccgaatgt gtgaaaacta gatgcattac
    16261 caaaataatc tgttaggtat ggcatcattg catccaatac ttctttttta ataggtgttg
    16321 ttgcagaata atccatatac aatcttcttt tttccattac attcatctcc aaatctattt
    16381 tttaatattt gttatatcat tttctaattt atttttgtta tagtcattta tcatatcttt
    16441 aagagtaatt gaatctataa catcttctat accctttttc atcttttccc aaactatttt
    16501 tgttacacac atgtttgagt tttcacatac atcttcatct agtacacagt cagataatgc
    16561 aactggtcct tctaatacta caagtatatc tcctactgtt atgtcttcag cattctttga
    16621 aagcaaataa cctccttgag cacctctcac actttttact agacccgatt tttttaaaga
    16681 tgaaaatatt tgttctaagt actgatctga tattttttgc ttcttagcaa taaattttaa
    16741 tgatactggt ccattgtctt gatttaatgc taattcaaac attgctttta gtccatatct
    16801 acctttagta gacaacttca tatattatca cctctatata gaaatctaat tcatactgtt
    16861 ttagtaggaa ttactttcaa gataatttta ttatacccta ctaattttgt caagattaat
    16921 tttaattttt tattttgaat aaattcccca ttttccaatg ttttcaaatc ctagtttttt
    16981 atatatcttt ccagcctctt cattatcata aaacaaacaa ggaattttgt tttctctcag
    17041 taactctgaa catagtttaa ttaggcattt tgtagcaagt cctttagctc tatatttagg
    17101 atgtgttcct actccaatta tcattgcatg agtcctattt tctgaagtac tctttgccat
    17161 tgacaccacc tgtttgttaa tctctataca atatcctcta cctgtcctta gtccattttt
    17221 taaattctct aaagttgtac tgtgaaattc atctattaag tcataaagtt tcactgtctt
    17281 ttttagattt ccaagcctaa ttctcttaac ctttgcatta caattattat ctatcaaaaa
    17341 ctttttagtt tgaagtttgc aaaaatcaac tacctttaaa ttattaagtc ctaatctctt
    17401 tgctaaagta tctacagcac atattttccc agatatttct tcgtaattag tattacgaag
    17461 tagattataa aaaccttcaa catcatactc tccatcagaa taaaacatca taaattcaaa
    17521 atatttaagt aatattgcct ttatttcacc atgttcaccc acgtctgccc atatatttaa
    17581 aaaattattt ccatatccat atctttcaat atcccctatt ataaacaaat tgtactcagg
    17641 ttcttttttt aagtatttca taactctact gtgataagtt gagttaagtt ttataatcat
    17701 aatatctacc cccgatacat aaaattcagt ctaaaaatca ctatttagat attctaaata
    17761 tttttttata tttttctcag ttttattttc ttgtggtatg taataaatac tatcttttat
    17821 gctatctggt aaatactgtt gctttacata atgattttta aaattatgag gatatttgta
    17881 tccacaaccc ctacctattt ctttagaacc agaatagtga gcatccttta tactatttgg
    17941 tatatcctta acaaactctt tatccagttc atccatagct ttcattattg ctatacatgc
    18001 agaattagac tttggagatg tagccaaaag aatagtagct tctgctaatg gtattttcgc
    18061 ttctggaaat ccaagttgca tagctgagtc tacacatgcc tttacaatta caatagcatt
    18121 tggataagct aaacctatat cttcactagc tattactaaa agtctcctac atatacttat
    18181 caaatcccca ccttttataa gtcttgctag atagtgtata gatgcctgtg ggtcacttcc
    18241 tcttatagat ttttgaaatg cacttaaaat atcataatga ctatcaccat ttttgtcata
    18301 gtttataatt ttattaaatg tagatgccct tattacatct gaatctatat aagttatatt
    18361 atccttattt ggtttagttg aatagactac cacttccaaa atatttaagg ctcttctcat
    18421 atctccatca gaaagaattg ctatatcttc aatagcatta ttattgcact caatatccat
    18481 atagctatct tcatttaata cttcaacagc tcttttcagt cctttttcta tatcaagttt
    18541 ttctaaaggt ttaaattcaa atacagtaga cctacttaaa agagctttat atacataatg
    18601 atatggattt tctgtagtgc tagcaatcaa agttatactc ccattttcca taaattctaa
    18661 tattgattgt tgctgttttt tattaaaact ttgtatttca tctatatata ataggactcc
    18721 atttacatta tttatacttc caagctctga tatcaccttt tttatatctt ctaaagatga
    18781 attagtagca tttattttat agaagctttt attgcttctt tctgctatta tttccgctac
    18841 tgtagtttta ccaacaccag gaggaccaaa aaatatcata tttggtaaaa aattagattg
    18901 taatatctta gacaatattt ttccattacc tattatatga ctttgaccta ttacatcatt
    18961 cattgttttt ggtcttattt tatctgctaa aggcattttc tcactccttt ttaatatact
    19021 tatcattacc ttaataattc gtctaaaaaa ggtatctatt tgaaatagat accttttcta
    19081 atctaaacct ataaaatata gattattgat tgttttttaa tttttttaat tcatctgtaa
    19141 gtttgtcatt aagtattctt atatgagtac ctttcatacc taatgatctt gattctatca
    19201 cacctgcact ctcaaatttt ctaagtgcat ttactattac agacctagtt ataccaactc
    19261 tatcagctat cttacttgct acaagtagac cttcttttcc atccaattca gcaaaaatat
    19321 gttcaactgc ttcaagctcg gagtaggaca gagtgcctat tgccatttga actacagctt
    19381 tctttctcat ttcttcttct aattcttcac ctattgctct taatatttca agaccaacaa
    19441 cagttgcact gtattcagct attactaaat catcatcatt gaatgaattt gaatatcttg
    19501 aaagtaccaa tgttcctaat ctttgaccgc ttcctaatat tggaactact gtagtatatt
    19561 tttgtaatct tccatgttct tcaggaaaga tttctaatat cttctcacca tttaaatttt
    19621 ctaatgtttc atcaattttt aacacatttt gagtatattc atctgaaaat ttcttttgct
    19681 tagtatactc atcttctata actgaactgt cttgaacatc atttaaatga agacctagta
    19741 ctttaccttt tgcacttact acataaacat tagaacttaa aacgtcgccc aatgctccgg
    19801 ccagtaaatc aaaagagaca ctgcttccac cacttgtttg taatgtttta tttattttcc
    19861 ttgttttttg taacacttca cttgccattt aatcatctcc tcatgaaatt taggcctttt
    19921 aataaccttt tatattttct aatattattt tcacatttaa tgaaaattta tttttctatt
    19981 agttgtttac acttgaaaga ttatcataga ttggaacctt tttcaagaga attatgtgat
    20041 ttttttcaat tttcactctt ttccgtcatt atgctctttt gtctctttat tacattcttt
    20101 attagaacac actgtttttg tttcagattt agtcactttt tcaaccatat atgaaccaca
    20161 cttctcacaa aactctcctg ttggcttatt ccaagctaca aagtcacagt ttggataatt
    20221 agaacaccca taaaaagctt tacctctttt agattttcta agtataatat ctccttcttc
    20281 acattttgga catttaacac caattttatt cataattggt ctagttgttt tacaatcggg
    20341 atagttttta caagccataa actttccaaa tctaccatat ttgataacca tattagaccc
    20401 acagttctcg caaatctcat cagtttcttc atccatattt actttttcta tattttcaag
    20461 agcttcttca atagcctctt taagtggtgc atatgattct gatacaatct ttttccaatc
    20521 tgtattgcct tcttctataa catcaagttt actttccata tcagcagtaa aatctacatc
    20581 tataaattta tggaaattat tttccaaaat atcagtaact attattccaa gttctgttgg
    20641 acatagactt gttccttgct tttcaacata ctctctattc agtatagtag ctattgttgg
    20701 agcatacgta cttggtctac ctatacctag ttcttctagt gtttttacta aactagcttc
    20761 tgtatatctt gctggtggtt gtgtaaaatg ttggtctggc aatatatttt ctatatttaa
    20821 tatatctcct ttttctatac ttggtaaaat tttgtcttct ctttctgaaa aattatatac
    20881 cttggtataa ccatcaaatt tcatttttga accagttgcc ttaaatacaa tatcgcctat
    20941 tttacattct acatttagta tatcaaacac tgaatcttcc atttgactag ctacaaatct
    21001 tctccaaatt aaattgtata acttgtactg gtctttgctc aatgaggctt ttatactctc
    21061 tggagttcta tcaactgagg ttggtcttat tgcctcatgg gcatcttgaa cctttttagt
    21121 atcctttttt tcttttttat caccagtgtt tttatagtag ttttctccta aattgtttac
    21181 gatataaccc ttagcttttt cttttgcttc atcagatatt cttttagagt cagttcttat
    21241 atatgatata agaccaacag taccttcacc ttctatgtcg ataccttcat ataattcttg
    21301 tgctatcatc attgtttttt tagtagtaaa aaataactta tttgctcctt cttgttgaag
    21361 catacttgtt gtaaatggtt ttggagctga tttacgtctt gatttagatt ctatcttctc
    21421 aacctttaaa tctttacctt ccatttcttt tagtatttca tttacagctt cttcattttc
    21481 taattcaatc ttttcatcat ttttaccata aaatttcaaa gttatatctt tgtcttttac
    21541 tgtcttagcc tcaacatcta ttgtccagta ttcttttgga ataaatgcct ttatttcttt
    21601 ttctctatca caaattaact ttgttgtaac agattgcact ctacctgcac taagaccttt
    21661 tctaaccttt tgccataata taggacttat ttgataacct aaaagtctgt ctagcactct
    21721 tcttgcctgt tgtgcatcaa ccaaatttat atcaatattt ctaggatgtt taattgcttt
    21781 tttaattgca tccttagtta tctcattaaa ctctattcta caatcctcag attcatctaa
    21841 atttagtata tgagctaaat gccatgatat agcttcacct tctctatcag ggtcagttgc
    21901 tagatatact tgtttagatt ttttagcttc tttttttaat tcttttatta catcgccttt
    21961 tcctcttata tttatatatt gaggttcaaa attattttct atatctacac ctaatttact
    22021 tttaggtaaa tctctgacat gaccaactga tgctttaact gtgtaatgac ttttccctaa
    22081 aaatttttct atagttttag cttttgcagg cgattctacg atgactaatg ttttggccat
    22141 aaaaccatac acccccacta cctaatctta atttatacat ttaaactgta cgttttatta
    22201 ttcatttcta ctactaactc atttagtact aattcattga ttattgaatt tacatacttt
    22261 atttctatac cagtataatc acaaatacta tctatatgca agtttccttc tctttttata
    22321 gcttcaatta tttgcttaga cttggcattt agacttatat tatcatattt ttgatttatt
    22381 tttttatcaa taatattaaa tatgttatat tcatttaaaa tatcatctat attttcaatt
    22441 aattttgcac cttcttttat aattttatga caacctctac tcatttgaga atttatatta
    22501 ccaggtattg caaaaacatt ttttccatgt tcaagagcaa aatctactgt gattagtgca
    22561 ccacttttta ttgctgcttc tacaacaact actccatcac ttaaaccact gataattcta
    22621 tttctattag aaaaatggta cggggctact gtcgagttaa tattatactc tgataataat
    22681 aatcccccat tttctaatat tttatttgat aaatgtaaat tttgttttgg aagaggattg
    22741 tctactcctg aaccaagtac tgctatagtt ttagattttc cactcataca acctatgtgc
    22801 gagtatgcat ctattcctat agctaatcca cttactatat taactccata ttgggatagt
    22861 tgacaactta ttgtttttgc acaattaatt ccataggctg taggttttct tgaacctact
    22921 atagctatat taaaattatt attaactaaa cctatgtcac ctttgtagaa taataacttt
    22981 ggagcgttgt atatattctt taaattttca ggatattctt tatcatattt acaaatatat
    23041 ttaatcgctt ttttgtaaag tagttctttt atattttcta aataagcgta acctctatat
    23101 tttactatat tttttctaat attcaagctt atatttttta aattatatat ttctttttca
    23161 gaaaaatcaa atatatcttc aatatttact atttcatttt ctattatttc aattgttttt
    23221 gttgttattc caccaattga ttttaaccat aaataagtat ctcttttttc cataaaatcc
    23281 acctcaaaaa actatttaaa ataactataa taagcctttc taaatgaaaa agcttctata
    23341 atacactgtg aatttatcaa gtctctttct tctaaatctg ctattgttct tgccattttt
    23401 aaaagttttg tataacttct actgcttagg ttgtatttat taaatatcat ctcagccgtt
    23461 tttgaagctt ctttttccag tttacaatat ttaaataaat tgtaagcttt aatttcacta
    23521 ttatttttta tattatcttt cttaaatcta tttatctgta tttttctagc attttctact
    23581 cttctcttta ttttttgaga gctttcttct tgttttaaag aattaaaatc tgaatatttt
    23641 atagaattaa cttctacaaa aatatcaaac ctatctaaca atggacctga tattttattt
    23701 atgtatctat caatttcgtt acttctacat ctacactctg tctctgacat ataataacca
    23761 catggacatg gattcatagc tgcaactaac agtactctac aaggatattt tgcactgtac
    23821 tttactctgg atatatttac atatccatct tctataggtt gtcttaaagt ctcaagtatt
    23881 tttctatcaa attcggctat ctcatctaaa aataagatac ctctatgtgc taaagcaatt
    23941 tctccaggtc ttgcatccat tcctcctcca attaaagatt gctttgtagt tgtatgatga
    24001 ggtgctctaa aaggtctttt atcaattatt cctttacttt catttatcat acccaatata
    24061 ctataaacct tacttacttc tatcatttct tctatactta tatctggtaa aattgtcctt
    24121 actctttttg caatcatagt ttttcctgag cctggaggtc caatcataaa catattatga
    24181 tttccagctg ctgcaatttc tgcactcctc tttacaaaat aatttccttt tacatcttta
    24241 aagtcttcat catatttact ttcttcatta ttaatcttag tatatttgca atctatataa
    24301 ctattatctt tttctaattc tccgttctct tttcttgact tatcatccaa aaaagacatt
    24361 atacttactt tatcgacttt tatctcttcg tttaaaaaat cgacacattc ttttaaactt
    24421 ttaataggaa ttatatcaat ttcatctact aatagacttt cttttatgtt ctcaattggt
    24481 ataaaaatac gttttatgtt ctgagttttt gcacccataa ttatgggcaa tatccctctt
    24541 acttttctta tttttccatc taatgataat tctcctataa acatactttc tcttatgtaa
    24601 ttttcatctt tttttattaa ctctcttaaa aggcctatac ttatagataa atctaaaaaa
    24661 gctccttctt tttttatatc tgctggtgat aaatttacaa ctattctcgt gcttggaaat
    24721 ttatatccac tattaagaat tgctgacttt actctttctc ttgcttcttt aatttctttg
    24781 cctggaagac caacaatatt aaaactaggt attccattgc ttacatctac ttcaacctta
    24841 actaaaaagc tgtcgattcc tactaagttg cttgaattaa taatacttaa catatttaat
    24901 cctttccaaa aacattatct tttttaaaaa gcattcatta tgtgatttat tcttttttct
    24961 aacaaataga cctctatgat gtcaaatctt atgggagtat tatttaagtt attttttata
    25021 atatagtatt ttgcgacacc aattatcttt tttctttttt taaaatctac agattcacaa
    25081 gggtagccat actttatgtt agaccttgat ttcacctcca caaatacaac ttcatctcct
    25141 atatttgcta ttatatctac ctcccctatc cttaatctat aatttttttc tagtatcttt
    25201 gcaccttttg atagcagata attaactgct atttcttctc caaaatcacc tttttcttta
    25261 ttattcatta tctactccaa tatattaaat ttttactttt aaattatcgt cagtatacaa
    25321 tttaactatt caattttttg aaattgattt tatttccatt gatattaata aatacttcta
    25381 ttgactttat tcttttatga tattatgcta gtttttataa tgaaagttta tttttatgga
    25441 ggaatgtctt taatgaacag aaaaaaaaca tttattttta tacgatattt atctctattt
    25501 atattactta taagttattt aaaccatact gaaaatacat tgtatccagc tataacaatg
    25561 attatattat ttctaataat aaataatcaa gttagatttt tttctttatc aagtgagaat
    25621 aaatttatac ttatttctta ttttttagag cttattctaa tttttatatt ttgtaagtat
    25681 ataaagactt ttaatccact ttactatgtt tctttaattt tggatgcctc atttttaata
    25741 aaggaaaaat ataaatatat attattctct ttagttataa tattttcttt tattattggt
    25801 ttagataaaa atttatattt agcactagaa tcaagttcta cattactaat tataaccctc
    25861 ttttctgtgt atctagaaaa cgagaattta tctaaattat acaatcaaag tctttatgac
    25921 aaactcaggg tttctaaaga tgaattaaaa aaagctaatg ccgatttaga gatttatgca
    25981 aattctatag aagagttagc aattttgaaa gaaagaaacc gtatatcgag ggaaatacac
    26041 gatagtgtgg gacattctct ttctacgacg ataatccaat taaatgctat agaaaaactc
    26101 ttaaaagaca aacctctcat ttctgattta gttcatgaac ttagagagtt tgtaactgaa
    26161 agttttcaag atgtgagaag agctatatct gaactaaaac ccattgaata tgaaaattac
    26221 caaaatttac ttaaaatcag agaactggtt aatagcttta ttaaacttac aaatatcaac
    26281 gttagattaa ctatatcaaa aaatacctgg aacctatcta caaatcaatc tatcgcttta
    26341 tatagacttg ttcaggaatc tctttccaac gcatcaagac atggaaaagc aactgaaatc
    26401 agaattttta taacatttaa tccttcaaat cttataatta ctatcagcga taatggtata
    26461 gggtgtaaca atataaaaag aggaaatgga cttagcagta ttagcgaaag aatatatgaa
    26521 ttaaatggta aagttgaatt cgataattct aatactggat tcacaattag agcgtctttt
    26581 cctaaatttt caggaggtga tttttttgaa taaaatcaaa gttcttatag tagatgatga
    26641 aaaattgatt agaaaaggtc ttaacattat tttatcatct tatgacgatt tagaaattgt
    26701 aggagatgct tccaatggat atgaagcatt agaattttgt aaaacaaatg atgtagacat
    26761 agttcttatg gatattcgga tgaaagtttg tgatggagtt ctaggcacaa gacttataaa
    26821 agaatatgat agctctatag cattattaat tttgacaacc tttaatgatg atgagtacat
    26881 agaagatgct atgaaatttg gggcttcagg atatctttta aaggatagtt cggatgaagt
    26941 attacatgaa gggattcgtt cctcgttttt tggaaatatt gtacttgata aaagtgtggc
    27001 tgaaaaaatt atgtaccctc aaaaaactat agaacaaaaa catatttctg atatgtataa
    27061 tttaacagaa aaagaaatat ccataattag attaatcgca aatggactta acaacaaaga
    27121 aatcagccaa gaattattct tatctgaagg tacaataaag aataatatca ccaatatctt
    27181 agggaaacta gagcttcgtg ataggacaca actagttata tttgccttca aaaataaaat
    27241 tgtaatataa atatataggt aagaaatcta taccctagaa atttcttact cacaaactca
    27301 tttataataa atgaaaatat aaagtgactt aagtcacttt ttttagtgac ttatagtaat
    27361 agaattatga ttcataattc tttattatag tattatagat ttttaactta tacaaaatta
    27421 ttttgggagg aattattatg gatatagtaa gagtaaataa tataacaaaa agatttaatg
    27481 ataaattagc acttgataat atcagctttt ctgttaaaga aggagagata tttggactta
    27541 ttggaccgaa tggtgctggt aaatctacac ttataaatat aattacaaat cttatgttac
    27601 caaatagtgg cgacatccaa atcaatggtt tagatttgtc aaaagattgt ataaaagcaa
    27661 agtctataat aggattagtt ccacaagaat tagcacttat agagactctt actccttttg
    27721 ataacttaga gtactttggc acttactatg gactaaaggg aaaattgctt caagagagaa
    27781 taactgaagc cttagaagtc acaggactca ctgatgttaa aaagaaaaaa atcaaaaaat
    27841 tatctggtgg tatgcaaagg agattgaaca ttggaatagc acttttaaat catcctaaaa
    27901 tattaatatt ggatgaacca actgtaggtg ttgacccgca atcaagaaac catattttca
    27961 attttataaa aaatatatct aaaaagcaaa aaaccacagt aatatatact tctcattata
    28021 tggaagaagt tgagcatctt tgttctaaga tttttattat ggatgaaggt aaagagattg
    28081 cttttggaga taatgattat ttaaaatcat tagtatctac aaatacaaaa ttaattatgg
    28141 aaataaaaaa tataaatgct caacttatat ttgatttaaa aaatactaaa ggtgttgtat
    28201 ctattttaga aaataattca ttattagaat taggtgtaga caaaaaatta caacttacag
    28261 atgtattatc tgttgttgac aaaaataatt caaaaataac acgaatatct tatgaagaac
    28321 ctagtttaga agacgtattt ttaaatctaa caggtaaaaa tttaagggac taatggaggt
    28381 gatttcatga aattgtacat tagtgtaaaa actatgctaa aagttttaaa aagctccttt
    28441 atgcttaatt taatatattt cttagcctta cctctagttt tagctggatt tcttgggatg
    28501 gtgacagaaa gtatatttca aaatcctata aaaacagatt tgacatcaat tgttatatat
    28561 gataaagata atacaaagct atcaagtaat ttgacaggat atctaaaaaa cgatttatct
    28621 tctatatt
//
LOCUS       DAEPLF010000027        27858 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEPLF010000027 DAEPLF010000000
VERSION     DAEPLF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.027"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            44..1006
                     /locus_tag="KR314_001079"
     CDS             44..1006
                     /locus_tag="KR314_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBH0327477.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHIFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVLSPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(1124..1786)
                     /gene="zmp1"
                     /locus_tag="KR314_001080"
     CDS             complement(1124..1786)
                     /gene="zmp1"
                     /locus_tag="KR314_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBH0327478.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHAYSTTIQQNKDTLSQIVVF
                     PTGNYNKNEANAMVNRLANIDGKYLNVLKQDNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAEAFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(1886..1971)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2157..5012)
                     /locus_tag="KR314_001081"
                     /pseudo
     CDS             complement(2157..5012)
                     /locus_tag="KR314_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
     gene            5078..5154
                     /locus_tag="KR314_001082"
     tRNA            5078..5154
                     /locus_tag="KR314_001082"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5112..5114,aa:Arg,seq:cct)
     gene            complement(5363..6808)
                     /locus_tag="KR314_001083"
     CDS             complement(5363..6808)
                     /locus_tag="KR314_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
                     /protein_id="HBH0327479.1"
                     /translation="MASIKQRKSSFSVIYWYLDSAGERKQKWDTLETRKEAKQRKAFI
                     EYYQEKFGYVIVPLEEQFAHQIENSKKELDVVDKDITLRDFLEIFVNLYGTSKWSPST
                     FSSKVGSIENYINPIIGDWKLNEITTKSLSKYYNDLLSVPEVPRANRKATGRCVQPAN
                     IKKIHDIIRCALNQAIRWEYIDTNKRNPASLATLPKIPKTRRKVWSVQTFREAVKATE
                     DDLLSICMHLAFSCSMRIGEITGLTWEDVIIDEESIATNNAKVIINKELSRVNAEAMQ
                     KLKEKDILKIFPTQKPHCTTRLVLKTPKTETSNRVVWLPKTVAELLVQYKKDQQELKE
                     FLGSAYNDYNLVIALDNGNPVESRIVRDRFQKLCEENDYEVVVFHSLRHLSTGYKLKM
                     TNGDVKSVQGDTGHAEAEMVTDVYSEIIDEDRRFNAQKMDKEFYSTLNDDTDNPKQDT
                     DLTDSDMALLELIKSLSPEMKEQFLKQALQR"
     gene            complement(6827..7228)
                     /locus_tag="KR314_001084"
     CDS             complement(6827..7228)
                     /locus_tag="KR314_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0327480.1"
                     /translation="MIRPHGAVLPTRMGIVEILTELSKAVCLLIIDSFSNKIYLLFLF
                     RCVIMTLLDECILLLSQMKGVLIVISDTQNAQSPTKEKKTYTVEEIAEQLDISKKVAY
                     SLVKSGQFSYVRAGKAIRVSKVSFDKWLNEI"
     gene            complement(7479..7727)
                     /locus_tag="KR314_001085"
     CDS             complement(7479..7727)
                     /locus_tag="KR314_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0327481.1"
                     /translation="MKHTMKKPSYYLLSQAMDGDEKAIEKILAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGLIREALIKMILGFDIALEIEEE"
     gene            complement(7734..8150)
                     /locus_tag="KR314_001086"
     CDS             complement(7734..8150)
                     /locus_tag="KR314_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0327482.1"
                     /translation="MKPSDFQKTVQCRFESCLKKVVRHVVKDYQKKLKRRQKGETLFC
                     ELPEIVVENLAVWDDYDTDYTIFNVCGSDIRICDDELAEALKHLSERNRENLLMYYFL
                     EMSDTEIAKRQNISRSGVFQNRHNSLELMKKILKEK"
     gene            complement(8598..9476)
                     /locus_tag="KR314_001087"
     CDS             complement(8598..9476)
                     /locus_tag="KR314_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBH0327483.1"
                     /translation="MIQYYYTKKEWGVVMEKEELKILEELRRILNSKNEAIVILNNYF
                     KGGVGKSKLSTMFAYLTDKFNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSTTIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLAYSMQ"
     gene            complement(9579..9863)
                     /locus_tag="KR314_001088"
     CDS             complement(9579..9863)
                     /locus_tag="KR314_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HBH0327484.1"
                     /translation="MPTKRSGPLVGKCPKCGNNIVLKKSFYGCSNYPECTFTLAEHFR
                     KKKLTKTNVKELLEGKETLVKGIKTKDRKSYNAVVKIGEKGYIDFISFSK"
     gene            complement(10009..10746)
                     /gene="erm(B)"
                     /locus_tag="KR314_001089"
     CDS             complement(10009..10746)
                     /gene="erm(B)"
                     /locus_tag="KR314_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBH0327485.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(10871..11212)
                     /locus_tag="KR314_001090"
                     /pseudo
     CDS             complement(10871..11212)
                     /locus_tag="KR314_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(11273..11422)
                     /locus_tag="KR314_001091"
                     /pseudo
     CDS             complement(11273..11422)
                     /locus_tag="KR314_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            complement(11424..11696)
                     /locus_tag="KR314_001092"
     CDS             complement(11424..11696)
                     /locus_tag="KR314_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBH0327486.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(11713..11928)
                     /locus_tag="KR314_001093"
     CDS             complement(11713..11928)
                     /locus_tag="KR314_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBH0327487.1"
                     /translation="MVVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDKALEEYIRKYLPDKL"
     gene            12115..12474
                     /locus_tag="KR314_001094"
     CDS             12115..12474
                     /locus_tag="KR314_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0327488.1"
                     /translation="MRKAKETQTFDFRPLGLAIREARERAGLSRNDLGDKVFYGERHI
                     ADIENVGSHPSFQLFHDLVTMFNISVDEYFYPAENVVKSTARRQIETSLDLLSDNELK
                     IIQGTIDGILNSRESKK"
     gene            complement(12582..12770)
                     /locus_tag="KR314_001095"
     CDS             complement(12582..12770)
                     /locus_tag="KR314_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBH0327489.1"
                     /translation="MDKQLWVLCPICNNKTRTKVREDTELINFPLFCPKCKTETLINI
                     KHGKVTIIKEPDAKTQSR"
     gene            complement(12975..13166)
                     /locus_tag="KR314_001096"
     CDS             complement(12975..13166)
                     /locus_tag="KR314_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HBH0327490.1"
                     /translation="MRKCLRCHEEMVENLDVKVDMQGYGIRITTKGVFGTTVEKPKVA
                     VCPKCGEVSLYIENFKSIK"
     gene            complement(13179..13823)
                     /locus_tag="KR314_001097"
     CDS             complement(13179..13823)
                     /locus_tag="KR314_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBH0327491.1"
                     /translation="MKGLLMKDLLVIQKKYGITRIIMDIFIIVALMLFLKGTGAIYIS
                     LLLIPVEIMTMIISLTTCDEQWKWGKYVVALPVSKTTIVKSRYIVALIMTCIGFGVAL
                     LVNTITYFIFPQFAFGYYMFIACASFAVTLVFLSFVLPSNYSLGVNAGFAVMFTLVII
                     LVVLAVWTNVTNNSILWFVVDNFELSIGIAFIIVIALAFSSYLLSKAFFTRKYN"
     gene            complement(13820..14683)
                     /locus_tag="KR314_001098"
     CDS             complement(13820..14683)
                     /locus_tag="KR314_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327492.1"
                     /translation="MAENILEVKNLTKDYGDFVLNDLSFSIPRGVIMGLIGENGAGKS
                     TTVNCILNEIKKNSGNILIFGKDHIIDECEIKDKIGIVFDENHFPDIFTPKEIEKYMS
                     GIYTNWDSIAFRNYLKQFELPAKKKIKDFSKGMKVKLAFAVALSHKAELLILDEATSG
                     LDPIIRDDILDILIDFVQDENYSILFSSHITSDLEKIADYITFIHKGRLVFCSLKDDL
                     IDNYGVVSCGASVFDTLDKTEIISYQKEDYQYKVLVRDRNKIAKDFPKALVEPATIEN
                     IMLFYVKGELQ"
     gene            complement(14684..15061)
                     /locus_tag="KR314_001099"
     CDS             complement(14684..15061)
                     /locus_tag="KR314_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0327493.1"
                     /translation="MEIFINNHASIPIYEQISSQIKALIINGELKAGEALPSIRGLAK
                     SLHISVLTVQKSYDVLQKDGFIETTAGKGCFVSAQNQDFYIEEQQKKIEDRFVEAIEI
                     ARASGINLDKLIGLLKLLYEEDE"
     gene            complement(15182..16093)
                     /locus_tag="KR314_001100"
     CDS             complement(15182..16093)
                     /locus_tag="KR314_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0327494.1"
                     /translation="MFKKNKKQTETIKEPKEKKVRTVKVGTHKKTVIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWNNSKEAIEARTQA
                     ISGYLTKELQDLNIDTIRTDIPTSSTVTDVIVWHIEQSGTDTFSATYEVDQQIKEGEQ
                     TSNVKATYTVKVHVDADGDMVIVQNPTLAPAIEKSDYDPKTPETDASVDADTANDATA
                     FLETFFKLYPTATENELAYYVSGNVLEPIGRDYLYSELVNPIFTKDGENVKVKVAVKF
                     LDNQTKATQVSQYELVLHKDSNWKIVG"
     gene            complement(16109..17116)
                     /locus_tag="KR314_001101"
     CDS             complement(16109..17116)
                     /locus_tag="KR314_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBH0327495.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAREYGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGMDDLNVAIQSYNYGGGYVGYVAGKGNKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYSYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGFADLSSSYWQQHLIGAGRVK
                     Q"
     gene            complement(17113..19308)
                     /locus_tag="KR314_001102"
     CDS             complement(17113..19308)
                     /locus_tag="KR314_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBH0327496.1"
                     /translation="MKERIKGAFTKKKIFHFLKMVLFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTIAGVTQNGFSSTGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSKGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYLAHRARRMERRLAGAVTAGGVAGAVAGSTISNRKATRNTATKDSRGNTSSSMGQR
                     VGSKVGAVLDTKNKVKDKASAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGMVQE
                     QQSRQSGRMEKREQHRQNIADKRMELQKAQEARQAQRKADGSATTGATRPHERPATAS
                     TIPKPSAEKTQEVKRPATATTSKTNEPVKTTVIKERPLSSGTSDRKTSQPTQPVHRQN
                     VEKVVSQETRQNDTKDRRTKVQQTQSVQKNQQTTEKTRNLVMKKGQKKK"
     gene            complement(19308..21758)
                     /locus_tag="KR314_001103"
     CDS             complement(19308..21758)
                     /locus_tag="KR314_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0327497.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIHSMQEQSKKLVTGKLKEVACQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRNGIAYEDYEYQLPKKKLNKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVVRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGIKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKGNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSNSRGLLHVIDELRREDTPISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            complement(21736..22134)
                     /locus_tag="KR314_001104"
     CDS             complement(21736..22134)
                     /locus_tag="KR314_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0327498.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQTLNETITAVRSVNYVPDKIY"
     gene            complement(22252..22755)
                     /locus_tag="KR314_001105"
     CDS             complement(22252..22755)
                     /locus_tag="KR314_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBH0327499.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GICEIPY"
     gene            complement(22773..23276)
                     /locus_tag="KR314_001106"
     CDS             complement(22773..23276)
                     /locus_tag="KR314_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBH0327500.1"
                     /translation="MQETRVLVETRETTGEETTSYWFDLPIDVAEFEEKLGVGAESTA
                     YRIIEKVLPYADEVHEHTSVYQLNELEFMYRQLSSDMQEEYVSLLTVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDSTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            complement(23195..23491)
                     /locus_tag="KR314_001107"
     CDS             complement(23195..23491)
                     /locus_tag="KR314_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0327501.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWREMKRLSPADYEERQL
                     EDFSKFVFGMPYQTLKDVMKQQKGREEQCRKQGCWWKREKQLAKKQHHTGSTCR"
     gene            complement(23576..24352)
                     /locus_tag="KR314_001108"
     CDS             complement(23576..24352)
                     /locus_tag="KR314_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0327502.1"
                     /translation="MSLGEKIFKLRKEKGISQEALAEQIGTTRQAISKWENNQGFPET
                     EKLLQLSNIFEVSTDYLLKDEKNVKNSEERGYYVSKEMSVGFIANQKKLGLYLALGVM
                     LCIFAGIPYTLFLDNMTWRYLGMAFCFVAGIIFFVVAIFYEKQEYNILRQEPLLLDYE
                     YLKELKSEYTSKKKKYVIVAVPCTVLFIAGLIFFLFTVKGYISWTEYHSFAFFGLAVG
                     MLGFVYTINVMETYELLVKNEQYSTSFFFKLKRKIKKRLG"
     gene            complement(24556..24777)
                     /locus_tag="KR314_001109"
     CDS             complement(24556..24777)
                     /locus_tag="KR314_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327503.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(24778..24912)
                     /locus_tag="KR314_001110"
     CDS             complement(24778..24912)
                     /locus_tag="KR314_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBH0327504.1"
                     /translation="MWVLLKDFLLVSMGMGIGIVLMCILNVGKEADYKMKQLEESEDN
                     "
     gene            complement(24925..26121)
                     /locus_tag="KR314_001111"
     CDS             complement(24925..26121)
                     /locus_tag="KR314_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBH0327505.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHSEDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEQQAKLTEKHEMIIKQQTTPA
                     KDLVEI"
     gene            complement(26305..27699)
                     /locus_tag="KR314_001112"
     CDS             complement(26305..27699)
                     /locus_tag="KR314_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0327506.1"
                     /translation="MRKIWNRGHRIRASDKHLVYHFSIGTLLFLFVAVLLLLNIKQLM
                     RTDWEHFSLLENGLTLSPYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLKLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMENVY
                     HTKEKMIDCVNAFYEGMVQRSEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAQARQ
                     DGTATCEAKGDGTD"
BASE COUNT         7795 a         5783 c         4541 g         9739 t
ORIGIN      
        1 tcttatctat agtactaata taaattacaa ggaggtgttt gacttgaaaa aaggttatgt
       61 ttatataata ctgactacct tattttttag ttcaatggaa attagtctaa aaactgtcac
      121 taatgacttt aatcccttac aaataacttt atcaagattt tttgttggcg gtttagtctt
      181 actacctttt gctgtaaaaa gattaagagc tcttagtctt tctattacaa aatcagactt
      241 aaaatacttt gcatttttag gttttatgtg tgtagtagta agtatgtcat tgtatcaact
      301 ggctgttcta aacacaaagg catctgtagt tgctgttttg ttcagttgta atccagtctt
      361 tgtaatgctt ttagcttact taattttgaa ggaaaaaata tataggcaca atgtaatttc
      421 acttatatta gaagttcttg gaattatagt tataataaat ccattgcata caaaactaac
      481 tcttagtgga atattcttaa cattatcttc tgctattatt ttttcaatgt atactgtatt
      541 tggtaaaaga aaaaccttaa aatttggtgg aatagttgta acttgcttta gctttatatt
      601 tggtagtttg gaaatgttga tattggtttt actcactaaa attaatttta tcgcagatat
      661 attaaataaa aataaacttc atatatttgc aaatatccct atttttagtg gatatacctt
      721 acacaatatg cccattatga tttatgtatt cgtatttgtt acaggtgttg gttatgcact
      781 ttactttatg gcaatggaag ctacatctac tagtcttaca tctttagtat tcttctttaa
      841 accagtacta tctccaatac tagcattatt aatacttaaa gaaattatcc ctataaacat
      901 ggttatagga attttattga tagtaattgg ttcaataatt tctattatac ctacgataat
      961 tactcaaatg cataataaac acgttgaagc cttgtataag gagtagtaat tgataatcta
     1021 tcaagttata cataagacaa aatttattag atttaaacgc aatataaaag agatgctatt
     1081 ataatagcat ctcttttata tttattttaa atttattaaa gctctattta gctaggtttt
     1141 gcaaaaagct ataagtttgt ggacaagctg attttaactt actatttgta tcttgattta
     1201 aatagtaata tgcaaatgct tctgcaaaaa attcttctgg atatacacct aagtagttta
     1261 cattacctaa agaacgacct tctttagcaa atatttgttt gaattgtgct gatttagata
     1321 tatcatttaa tactatatgg tctattgcat gtgctgtttc atgtaattct aaatttattg
     1381 catcatgccc tttacctttg ttactaaatc ctattctaag agctactgta cttccaccta
     1441 agcctggaac atcatcccaa gtttttccag taccttccca tcccttagga actacacctt
     1501 ttaaataagc atattctttt tcatctgtta actttccact taataatttt atttttaagt
     1561 tatcttgctt taatacattt aaatactttc catctatatt agctaatcta tttaccatag
     1621 cattagcttc gtttttattg taatttccag ttggaaaaac tactatttga ctaagtgtgt
     1681 ctttattttg ttgtatagta gtactatatg catgtgcact tactggaact gaacttatta
     1741 agaatattga tattatagct attaataatt ttttacttgg tctcatatta ttccctcctt
     1801 tgagttaggc cgggagtaat aaatttcaaa ataaaaaaga tacccaagag tatctttgta
     1861 tatacaaaaa gaaattttta ctcttttcgg caactggctg gcatggttta ctaaacttta
     1921 gcccctatag ctttgcgtga ctaggtttcc ctagttttgc ctcatttaat tttatatcta
     1981 tattatagct tatatctttt taattgtcta tatccctttg gaactttttt ctaaaaagtt
     2041 attatttttt tacataaatc ttcaaaaaaa aatatcttct aacaatattt tcattattaa
     2101 atattgtttt agaagatatt ttttaatata acttatattt tttataatgt tagggtctaa
     2161 tttgtatttt tatttcttct taatacgaca agtcctacaa tagctcctat aactaaaagt
     2221 atttctccaa taattgtagt actatcccct gtttttggtg gtactggtgg atttaccaaa
     2281 gtatcatctt tagctttatt atttttttct tctgacactg atggctttac cacttctggt
     2341 attggaacaa tctgtttaac aggagtttct atcacctctt ctgatggtgg tactggtgga
     2401 ttcaatatct ctggtattgg aggatttatt atatcttctg acggtggtac tggtggattt
     2461 ataactatag tatcctctgt atcagatgga tttgatggtt tccttggttt atctgtgctt
     2521 ggtggcactg gtggatttac tattggttca tctgtatttg gcggtacagg tggatttact
     2581 ggtggatttt cttgtcttat gttttctata tttccgatat ctactattct accaatatct
     2641 ttattaccaa tttcaacctt aattttatcc ttcagttttt catatccact tggagccatc
     2701 acttctacta aataatatat tcctggtttt tttacactag attgaattac accatcttca
     2761 tcactaacta aatccttaac tactatattt ccaaatgcat cttctaaatt gaacttagca
     2821 cctgaaagta catttttatc taaagcatcc acttttttag cttcaaaagc ataaccttgc
     2881 ttagtagtct ctactcccgg aggacttgaa ccattaatct gagccacatt ttgtatagtc
     2941 tccccaactt ttacatttgt gaagtctgca tcaaatgtaa cccagctttg cacaggttcc
     3001 ccttctttta tctgtttaaa ttcgtattta tttactaatc ttattgtatt ttttccatct
     3061 ccagcttctt ttttaagttc aagattatca gaatatttta tattagatat ttttagagat
     3121 gggtttactt catccaaaag atttacttgt cctgcatcat aaaccccata agttgacata
     3181 ttgattttgt atttaacaat ctggttatca ctactattta aaaccttttt atcaacttct
     3241 ttttttgcgc ttattaatcc agaatttttc ttaaagttta tctcatcatt tttatcaatt
     3301 tcactattat ttgcatcaag gacagctcta tttatataac tatcttcaat actaattacc
     3361 tttaatgtat aagctataaa aaactttgat tctacttctt ctttactgag attaattgta
     3421 aatccagtta taactttatc tacttgccca ctttcatttc ttttttcttc tactttatat
     3481 gaaatattat tggcaatttt ttctttactc caaggagtcc agctcccatt cattggaact
     3541 tctacaactc taaaatcatg cccatcctct cctgaagtag tcaatctcat accttctggt
     3601 atattatctg ttataacaac attacttatt ggtgatttat catcatttat tgaaatctga
     3661 tagtttatta cttgaccaac ttgagtaata tttgttgctc ctgaatgtcc tttagtaaga
     3721 agataattat ctcctctatc aaatatactt acagtagcat catcttcttg tatcttctta
     3781 ccatcagatt caatcctagc tacattctta tatttattac catgttttgc atttttgtca
     3841 ctgattctag ccttatactt aacagtatac gatttatcta tatttccaaa attaactgaa
     3901 atagaatttt tactctgttc aatattaaaa tcactaactt cttttccatc acttgttact
     3961 acacttacag aaccatttat caatgtcaat ccttcaggaa gctcatcata caaaataata
     4021 tcttttaact cttcctgctt ttcatttact tgtatactgt aatttatatc tttattttta
     4081 actggcataa atacagtagt tgattttctt ccttcaacaa gctttttaag tgttccttta
     4141 gcatctggaa catccgtttt atctccaggt tttatttcta catctatatc tccacttcca
     4201 ctttctattt ttccagtggt ttcaatatgt atttttgcat tagtatcttc atcaaccttt
     4261 ttaacatatg cttccaaacc tattttacct tgcattacac tttggccatt aggtaattct
     4321 ctaaatgtaa gtgtatactc tccattactt tctgttttat caaagcattc acttgtataa
     4381 tctaaattaa ctttattgaa tacatctggc accttaaaag ttattgaatc tcctgcttgt
     4441 attccttttg atgttggagt ataagttaaa ttaaatgata tttttttcat tgattccgtt
     4501 tctattgtat tgttattaat ttttacaaca aatccatagt cttcattaag ttcttcttct
     4561 aaaactttca ataaattttc atcttctaat agattatctt cctctggagt tttttctgta
     4621 tttttattgt ctagtggttt ttcatcttct aaagcttgtt ctgacttttt attatctgtt
     4681 gacttatctt cttctggtat ttgctctggc tttttattat ctattgactt ttctccttct
     4741 ggagtttgct ctggcttttt attatctatt ggcttttctc cttctggagt ttgctctggc
     4801 tttttattat ctgttgactt atcttcttct gaagtctgtt ctggtttttt attatctgtt
     4861 gaattatctt cctctggggt ctgttctggt tttttattat ctgttgcctt atctttctct
     4921 ggagtctgtt ctggtttttt attgtcgctt tccttctcct ctccagttga tgaattagtt
     4981 gaaccacttt gaatctgact attctctcct aagggctagg tggaacaaac gccttttgat
     5041 atgataacta gataaaattg aatagatttt taagaatgtc ccagtacctc agcaggatag
     5101 agggttcgcc tcctaagcga aacgtcgcag gttcgactcc tgtctgggac gccacaaact
     5161 ccgctgaaag ccttatttta caaggttttc agcgtttctt tatttatggc tttctattta
     5221 cgctgaatac gaattgtttt caatctgtca ttttttattc gatatttttt taagactttt
     5281 aaggaaatag gcttgctaac acttttttat ttttgaagaa aaatcgctgg tttttttgga
     5341 atttgctaac acttttgaaa ttctatcttt gaagtgcctg ctttaaaaat tgctctttca
     5401 tttctggcga taaagattta atcagttcca ataacgccat atcactatcg gttaagtcgg
     5461 tatcttgctt tggattgtct gtatcatcat taagcgtaga ataaaattct ttatccattt
     5521 tttgtgcatt gaaacgtctg tcctcgtcaa tgatttctga atatacatct gtaaccattt
     5581 ctgcttctgc gtgaccagta tcgccttgta cggacttaac atctccgttt gtcattttga
     5641 gtttataacc cgtacttaaa tggcgtaggc tgtgaaatac cacaacttca taatcgtttt
     5701 cttcacagag cttttgaaac ctatctctaa caatacggct ttccacggga ttgccattat
     5761 ccaacgcaat aacaaggttg taatcattgt aggcacttcc taaaaattcc tttaattcct
     5821 gctggtcttt tttatattgc accaatagtt ccgcaactgt tttaggtaac cagacaacac
     5881 gattacttgt ttctgttttt ggcgttttca agacaagcct tgtggtacaa tgcggttttt
     5941 gcgttgggaa aatctttaaa atatcctttt cctttaattt ctgcatagct tctgcattta
     6001 ctcttgaaag ttctttattg atgattacct ttgcattatt tgtagcaatg gactcttcat
     6061 caataataac atcttcccat gtaagtcctg taatttctcc aattcgcata gagcaggaaa
     6121 aagctaagtg catacaaatt gaaagcaaat catcttccgt ggcttttacc gcttctcgaa
     6181 atgtttgaac actccacact tttcgcctag tctttggaat ttttggtaac gtagccaaag
     6241 acgcagggtt tcttttgttt gtatcaatat actcccagcg gatagcttga tttaaggcac
     6301 agcggataat atcatgtatt tttttgatgt tcgctggttg aacacaacga ccagttgctt
     6361 ttctgtttgc ccgtggtact tctggaacgc tcaataaatc attgtagtat tttgatagac
     6421 tttttgtagt tatttcattc agtttccaat ctccaataat cggattgata taattttcaa
     6481 tactgcctac cttactacta aatgtactcg gcgaccattt agatgtgcca tataaattaa
     6541 caaaaatttc taggaaatca cgcaatgtaa tatctttgtc tacaacatct aactcctttt
     6601 tagaattttc aatctgatgt gcaaattgtt cctctaatgg aactatcaca tatccaaatt
     6661 tctcttgata gtattctata aaggcttttc gctgttttgc ttcttttctt gtttccagcg
     6721 tgtcccattt ttgttttctt tctcctgcac tatctaagta ccaataaata actgaaaaac
     6781 ttgattttct ttgtttgatt gaagccataa ttttctctcc ttatagttaa atttcattta
     6841 accatttatc gaaagaaacc tttgataccc gtatggcttt accagcacga acataactga
     6901 attgaccgct ttttactaat gaataagcta cctttttgct aatgtccagt tgttcagcaa
     6961 tttcctctac tgtataagtc tttttttcct ttgtaggaga ttgtgcattt tgtgtatcac
     7021 ttatcactat taagacccct ttcatttgtg acagcaacaa aatacactcg tctaataagg
     7081 tcattataac acacctaaat aaaaagagta gatagatttt gttgctgaaa ctatcaatta
     7141 taagcaggca gacggctttg gaaagctccg ttagtatctc aactattccc attcttgtgg
     7201 gcagaaccgc accatgcgga cgtatcatta ttctgtgagg ataggtcatg gcaaaacgac
     7261 ttttccaaca gtcgctctcg gatcactgcg tgggtcgctc gctttctttt gaaaaggtcg
     7321 tggcgtacag ttcccgtggc tcgctatctc acaaacgtat ctgtctgctt tattcagttg
     7381 tcaaagagct gtggcgaaag cgtgttgctt tcatataaaa gcaggggcag agcaggaaag
     7441 aggggattga acaaatcatg ctctgcggtt actgcttgtt attcttcttc gatttctaag
     7501 gcaatatcaa accctaaaat cattttaata agtgcttctc tgataagtcc ctttaattcc
     7561 atatctacaa caatatagac attgccgtat tcatcataga gtggtcgtag acagcacttt
     7621 gatatgtaag ggtcataaaa tgccagtatc ttttcaatcg ctttttcgtc cccgtccatt
     7681 gcctgcgaaa gcaagtagta ggacggcttc ttcattgtgt gtttcatttg ctatcatttc
     7741 tcctttagta tttttttcat aagctctagt gaattgtgtc ggttttgaaa aacaccactt
     7801 cttgaaatat tttgtctttt tgcaatctcg gtatcgctca tttccaagaa ataatacatc
     7861 aacaaatttt ctcgattacg ctcagacaaa tgtttcaagg cttctgccag ttcatcatca
     7921 caaatacgaa tatcactacc acatacattg aaaatcgtat agtccgtatc atagtcgtcc
     7981 cagacagcca gattttctac aacgatttct ggaagttcac aaaaaagcgt ttctcccttt
     8041 tgtcgtcgct ttaatttctt ctgatagtcc ttaacaacat gtcggacaac tttcttcaaa
     8101 caactttcaa aacggcattg aactgttttc tggaagtcag atggtttcat caatctcacc
     8161 ccctttccgt tacgatatga aaagtggtta tccctctttc cgcaccatat agggaacgac
     8221 aggtgtgatt tgctaagttg aaaccgaaaa aagttgaaaa aaatttttaa caaaacaaaa
     8281 acagcccgat aatgtgtaat tacacaaaca gactgtttta taaattctat tgatatgaat
     8341 ttttaattct tgtaaagggt gtaatgattg taatagtgga ctgaaatatt tattcagttg
     8401 agaacggcag aacgttcttc cgtatgcaat catggtatag ccccctttaa ccagagctat
     8461 gccttattta ctcctaagaa aaaaggacag ttctggttat atccaaaacc gtcccgttat
     8521 ttttcaagac gcacaaaagt gtattatcta ggaacggttt tttttattta ccgcacacga
     8581 taacagcttt tgaattatta ctgcatacta tatgcaagca tttcaaaaaa tacgttctca
     8641 tacatagata aaactttttt gtcatagcct ttgtgttctg taatgccgtt tttagaccag
     8701 gtacttactt tattacttcg cttgataata ttttggaaaa ccaagttatc ttctttatgt
     8761 tgcttgtaca gttcttccag gtttgatttt atcgttgtgc tgtccgtatc aactaaataa
     8821 ggaacaaaac cgatcatatc tagtccaggg ttaaactgtt cttgtaaatc aatcaaatag
     8881 gaaatatagt tttgaatgtt gtttgtactt tcttcttctg cttgtaaagg gatcatgacg
     8941 taatcacttg ccacgattgc attatttgta taaacgcttg gcgttggtac agtatcaata
     9001 ataatgagat catagtcact ttttaaaggt gctaaaagag tagcaagcaa tctactttca
     9061 ttttcaaaag tccatgagcg agttaatttt ggcagtaaca tcaaatcaaa cgtgccaggg
     9121 atcaagtcta aattatcagt caaatgaaca atagaagaag ccaagtttcc atttttcagt
     9181 ccttcataaa aattaacacg tggcaattct accttaaatg tttttgctaa gtcttttgtc
     9241 aatgttgctt gtaagtcctt atcgatcatt aaaactttta aattaaattt gtctgtcaag
     9301 taagcaaaca tagtcgataa ttttgacttt ccaacaccac ctttaaaata attgtttaag
     9361 ataacaatcg cttcattttt actgtttaaa atacgtctta attcttcaag tattttgagt
     9421 tcttccttct ccataactac gccccattct ttttttgtat agtaatattg tatcattagg
     9481 aatcacaaat cacaagtgat ttgtgattaa tcacttgtga tttgtgataa gtgatttgtg
     9541 attaatatat aaaagccctc tttaaagggc ttttatgctt attttgaaaa agagataaaa
     9601 tcaatatatc ccttttctcc gatttttaca acggcattgt aggactttct atctttcgtt
     9661 ttgattcctt ttaccagggt ttcttttccc tctagtaatt cttttacatt tgttttggtg
     9721 agttttttct ttctaaaatg ttcagctaaa gtgaaggtac attcaggata atttgaacaa
     9781 ccataaaacg atttttttaa tacaatattg ttgccacact taggacattt tcctacaagg
     9841 ggtcccgagc gcttagtggg aatttgtacc ccttatcgat acaaattccc cgtaggcgct
     9901 agggacctct ttagctcctt ggaagctgtc agtagtatac ctaataattt atctacattc
     9961 cctttagtaa cgtgtaactt tccaaattta caaaagcgac tcatagaatt atttcctccc
    10021 gttaaataat agataactat taaaaataga caatacttgc tcataagtaa cggtacttaa
    10081 attgtttact ttggcgtgtt tcattgcttg atgaaactga tttttagtaa acagttgacg
    10141 atattctcga ttgacccatt ttgaaacaaa gtacgtatat agcttccaat atttatctgg
    10201 aacatctgtg gtatggcggg taagttttat taagacactg tttacttttg gtttaggatg
    10261 aaagcattcc gctggcagct taagcaattg ctgaatcgag acttgagtgt gcaagagcaa
    10321 ccctagtgtt cggtgaatat ccaaggtacg cttgtagaat ccttcttcaa caatcagata
    10381 gatgtcagac gcatggcttt caaaaaccac ttttttaata atttgtgtgc ttaaatggta
    10441 aggaatactc ccaacaattt tatacctctg tttgttaggg aattgaaact gtagaatatc
    10501 ttggtgaatt aaagtgacac gagtattcag ttttaatttt tctgacgata agttgaatag
    10561 atgactgtct aattcaatag acgttacctg tttacttatt ttagccagtt tcgtcgttaa
    10621 atgcccttta cctgttccaa tttcgtaaac ggtatcggtt tcttttaaat tcaattgttt
    10681 tattatttgg ttgagtactt tttcactcgt taaaaagttt tgagaatatt ttatattttt
    10741 gttcatgtaa tcactccttc ttaattacaa atttttagca tctaatttaa cttcaattcc
    10801 tattatacaa aattttaaga tactgcacta tcaacacact cttaagtttg cttctaagtc
    10861 ttatttccat actcgctctt aatacaatat agtgtacctc gtaatcttct tgggcaagag
    10921 ctttccatgg ctccaaaaac catggcccga caatcccatc tacaattaca tcatatccgc
    10981 cacgagcata tcgctttgca gcctctaaaa aggcttcaat gacaaccaga ttttgcttgt
    11041 tggattctgg caaatgtggt ggtattgcgc ctttgcttaa gtaatgaaaa aagtcatcgg
    11101 tgtgcatatg cactgattta tccatatctg attcctttgc gacaattgac gcagttgttg
    11161 tttttcctgt ccccggtgag cctgtaatca caataattct tccttgattc atctatattt
    11221 tccctcacaa tttgaattta tcactttgtt cctgcctgct caatcatctt cttcgcaaac
    11281 tcgttttccc tgaccctggt tgcccaccaa gtaaaaaagc ggttggcgat tcaaccgctt
    11341 tttttccttg aaccaattct tctaaattat catttaagcg attttcaaat tgtttgtcag
    11401 taaaattgac tatatttgcc atattaagcc actttctctt tattcaaaac ttgttttgaa
    11461 tagcgattca ttgaccgcca atactcatga acggcttgta attcttctaa agaataatca
    11521 aaaagattta cacgttttgc aagctttaat tggtttaata aattgacttc caataattga
    11581 gagtcatttt tatttaattc atgattcaaa acatagttta atactcgatt ataaacgctt
    11641 gccgataatt cgttaatgat ctctatttca aatgtttttt cataagtaac tgccatttca
    11701 ttttcactcc ttttaaagtt tgtcaggtaa atatttccga atatattctt ctaaggcttt
    11761 atccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta taatatggtg
    11821 caagtcagca cgaacacgaa ccgtcttatc ccccataata tctttttttg cactgattgg
    11881 tgtatcattt cgttttgctt tttgtgcgcc taaatttccc acaaccactc acttctttct
    11941 atttcttctt actcttattt tatcatcaac aatcacaaat cacaagtgat ttgtgattaa
    12001 tcacttgtga tttgtgattc tattcttttt cgttttctaa ttgaatgatt cgctcaattt
    12061 taacacattc taaatataca gcatgaagta taaactcatg ctaggtgatt taaaatgaga
    12121 aaagcaaaag aaacacaaac ttttgatttc agacctttag gactggcaat cagagaagcc
    12181 cgtgagagag cagggctttc tcgtaatgat ttaggcgata aagtgttcta tggagaacgc
    12241 catatcgcag atattgaaaa tgttggttca caccctagtt ttcaattatt ccatgattta
    12301 gttaccatgt tcaatatatc ggtcgatgaa tatttctatc cagcagagaa cgtcgtaaaa
    12361 agtacagctc gccgtcagat agaaacctct cttgatttat tgagtgataa cgaattaaaa
    12421 atcattcaag gtacgattga cggtatcttg aacagcaggg agagcaagaa gtaatcagct
    12481 tcttgttttc cctttttgct attctgcatt atctatttgc tgatctgctc cgcaggtaac
    12541 aaataagagc caacaatcta taagtcataa taacctacaa attatcggct ctgcgtcttt
    12601 gcgtctggct ctttgataat agttactttt ccatgcttta tattgattaa agtttccgtt
    12661 ttgcacttcg gacagaacaa aggaaaattt atcagttctg tatcttctcg cactttagtt
    12721 cgtgtcttat tattgcaaat aggacataat acccataatt gcttatccat agccccccct
    12781 tattctttta agaaatttaa tttttgcttt ttcgtagtga tataaacata ttttttcggc
    12841 gtaaaatgtc tatttccaaa ataccttttg aaatttgctt gcatctctgg gtatgtacta
    12901 ttcataaatt cttcaatatc cgctcggacg ttagcaaaca ataaaccctc agtctatatg
    12961 cagattttaa tcatttattt tattgattta aaattttcaa tatataagga aacttcgcca
    13021 cattttggac aaactgcaac tttaggtttt tctaccgtag ttccaaaaac gcctttagta
    13081 gttattctaa taccatatcc ctgcatatca actttaacat ctaaattttc gaccatttct
    13141 tcatgacatc taagacattt tctcataaca atacctcact aattgtattt tctagtaaaa
    13201 aatgcttttg atagaagata agaactgaaa gcaagagcta taactattat aaaagctatg
    13261 ccgatactca attcaaaatt atcgactaca aaccataaaa tagaattgtt agtcacattt
    13321 gtccaaactg ccaatacaac aagaataatc actagagtaa acataaccgc aaaacctgca
    13381 ttaactccaa gtgaataatt tgaaggcaat acaaatgaca aaaatactaa ggttacggca
    13441 aaagaagcac aagcaataaa catataataa ccaaatgcaa attgagggaa tataaaatat
    13501 gttatggtat taacaagcaa agctacccca aagccaatac aagtcataat caaagcaact
    13561 atatatctgc tttttacaat cgtagttttt gaaacgggta gtgcaacaac atatttcccc
    13621 catttccatt gttcgtcgca agtcgttaag gaaattatca ttgtcattat ttcaactgga
    13681 attaacaata aactaatgta aatagctcct gtccctttta aaaataacat caaagctact
    13741 ataatgaata tatccataat aattctagta attccatatt ttttctgaat tactaataaa
    13801 tctttcatca ataagccttt cattgtaatt ccccctttac gtaaaacaac ataatatttt
    13861 caatagtagc aggttctaca agtgcttttg gaaaatcttt tgcaatcttg tttctatcac
    13921 gaacaagaac cttatattga taatcttctt tttgatagga aataatttct gtcttatcta
    13981 gcgtatcaaa gacacttgct ccacaactta caacaccata gttatcaatt aagtcgtctt
    14041 ttaatgagca gaaaactaac cgtcctttat gaataaaagt aatataatct gctatttttt
    14101 ctaaatcact tgtaatatgt gatgaaaaca aaatagagta attttcatct tgcacaaaat
    14161 caatcagtat atcaagaata tcatctcgaa taatagggtc caatccactt gttgcttcat
    14221 ctaatataag taattctgct ttatgtgaaa gagcaacagc gaatgcaagt tttactttca
    14281 ttcctttaga aaaatcttta attttcttct ttgctggtaa ttcgaactgt ttcaaataat
    14341 tacgaaatgc tatactgtcc caattggtat atattcccga catatatttt tcaatttctt
    14401 ttggtgtaaa aatatctgga aagtggtttt cgtcaaatac aattccgatt ttatctttta
    14461 tttcacattc atcaattatg tggtcttttc caaaaatcaa aatgtttcca ctgtttttct
    14521 taatttcgtt caaaatacaa tttactgtgg tcgattttcc agctccattt tcgccaataa
    14581 gccccataat tacacctcta ggaatggaaa aacttaagtc atttaataca aaatcgccat
    14641 aatcttttgt aaggttttta acttctaaaa tattttcagc catttactcg tcctcctcat
    14701 ataataattt taataatcca atcaatttat caaggtttat gccacttgca cgggctattt
    14761 caatagcttc tacaaatcta tcttcgattt ttttctgttg ttcttctata taaaaatctt
    14821 gattttgagc agatacaaaa caccctttgc ctgctgttgt ttcgataaaa ccgtcttttt
    14881 gaagcacatc ataagatttt tgaacagtca atacacttat atgcaatgat tttgcaagtc
    14941 ctcttattga tggtagagct tcgccagcct ttaattcccc atttataatc aatgctttta
    15001 tttgcgaaga aatttgttca taaataggta tgcttgcatg attattgata aagatttcca
    15061 tttaaacacc gccttgttct atactgtata gcttatacaa gtacagcata atacttaaaa
    15121 atacagttgt caatattaaa aggcttgcag agcataaaaa tatgatacaa gccttttaca
    15181 attatcctac aatcttccag ttactatcct tatgtagcac aagctcatac tgcgatactt
    15241 gcgttgcctt tgtctgatta tcaaggaatt ttacagcaac cttaactttt acattctccc
    15301 cgtcttttgt aaagataggg tttaccagtt cagaataaag gtagtccctg ccgataggtt
    15361 caagcacatt ccccgacaca taataagcaa gttcgttttc tgtggctgtt gggtacaact
    15421 taaagaatgt ttccagaaat gcggtagcgt catttgcagt atcagcgtct acacttgcgt
    15481 ctgtttctgg tgtctttgga tcatagtctg atttttcgat tgctggtgca agagtagggt
    15541 tctgaacgat taccatatcc ccgtcagcgt ctacatgaac ttttacagta taggttgctt
    15601 tcacattgct tgtctgttcg ccctctttta tctgctgatc tacttcgtag gtagcagaaa
    15661 aagtatccgt tcccgattgc tcgatatgcc atacaatcac atctgtaact gtggagctgg
    15721 tcggtatgtc ggttctaatg gtatctatat tcaagtcctg caattccttt gtcagataac
    15781 cgctgattgc ctgcgtcctt gcttcaatcg cttctttgct gttattccat gtgtaatatg
    15841 acttcgcaaa gttcttcacg aaattttcta tcccgttggt gtcctgcaag cgaagctcaa
    15901 tgatttcttt ttcatgggtg gtgtgctggt cgatagcggt aaaattctta tacaccccga
    15961 agctcacgct tgcgataagc accacccaca aggcaatcac agttttctta tgtgtgccta
    16021 ccttgacagt acgcaccttt ttttcttttg gttctttgat agtttctgtc tgtttcttat
    16081 ttttcttaaa catatcttca agtcctttct attgttttac tcgtcctgca ccgattaagt
    16141 gctgttgcca gtaactactg cttaggtctg caaatcctat cgggtcgcct gcatggtaca
    16201 tctggttatt tcctacataa atgccgacgt gggttacata cgaaccagcg ttataggtag
    16261 aatggaaaaa taccaagtcc ccagcctttg cctgcgagag tgggagatgt tgggtagcat
    16321 catactgtgc ttgtgccgtt ctcggtaagg agataccagc ttttccataa caccattgtg
    16381 tcagtcccga acagtcaaag gaagtgttgg gattactacc accatagacg tacttccagc
    16441 cttgatactt caacgcttca ttcattacct tttgtgccag ctctcccgac acctgcggaa
    16501 cagttaaata ctgattgact aattccacat agaacatatt tccatagcta taccgccagc
    16561 ctccattctt cgcaacagct attggattgg tgtatgttac tttctttcca cccgattttt
    16621 cacgggcaaa gctctctgca aggttgaaag tgtgtttatt tccttttcct gccacatatc
    16681 ccacataacc accgccatag ttataagact gtatcgctac attcaaatcg tccatacctt
    16741 gatttttgca ggacgaaagc agggacgcaa aatatttaca cccctgcttg attgaacttt
    16801 ctgtatctaa tgaattgggc ggtagtccca gactttcaga gctttgcata acgtcctcgg
    16861 ctgtaccgcc actttccact tgtatgattg ccagcaacac attgacatac tcggaaatgc
    16921 cgtattctct ggcgtacttt tccaccatag gctgatgttt caagacttct gcggataagt
    16981 tcatacccgt aatgccagaa gaaaagttgc tgttctcgtc gtcgctgtcc gcactaatca
    17041 acaccccaaa gaaaagcacc agtgaaaaga ggataggaaa cagactgccg ataatagcaa
    17101 tatgtttcag tttcattttt tcttctgtcc tttcttcatt acaaggttac gggttttctc
    17161 tgtggtctgc tggtttttct ggacgctttg cgtctgctga acttttgtcc tgcggtcttt
    17221 ggtgtcattc tggcgtgttt cctgcgatac cactttttct acattctgcc tatgtactgg
    17281 ctgtgtaggc tggctcgttt tcctatcaga agtacccgaa gataagggac gctctttgat
    17341 aaccgttgtc ttgactggct catttgtttt tgaagtcgtc gctgtggcag ggcgtttgac
    17401 ttcctgcgtt ttttcagcac tcggctttgg aatggttgaa gctgtggctg gtcgctcatg
    17461 gggacgggta gctcccgttg tcgctgatcc gtcagccttt cgctgtgcct gccttgcttc
    17521 ttgtgccttt tgaagctcca tacgcttatc ggcgatattc tgcctatgct gttcccgttt
    17581 ttccatacgt cccgactgtc tggactgctg ttcctgcacc atgccacgct tgaagtcgga
    17641 cacgctggac tttgcctttt cctttgcgga gtggacggca taagcggtct gtgtcggcat
    17701 atccttgata ttctctttga cagcacttgc cttatctttc actttatttt ttgtatctaa
    17761 gacagcaccc acctttgaac ctacacgctg tcccatgctg gaagatgtat taccccgact
    17821 atcttttgta gctgtgttcc ttgtcgcttt tctgtttgaa atcgtgctac cagccaccgc
    17881 accagcaaca ccaccagcag taaccgcacc agcaagccgt ctttccatac gtctagccct
    17941 atgtgccaag tatagatatg gtctgcggaa aattcttcgt cccatgcttt gactatcatt
    18001 agcgttcaaa gagaacatac tcattaagtc acccagcttc atgtagatac cagcgaaaca
    18061 cactatctgt aagaacgcca ccatgaaaaa cggatagtcc gtggatatgt tataaaacat
    18121 actggaaatg ctgaacgcca ccgttacaat gagcgttatt cctgctcgtg tcattatggt
    18181 attaaacacc ctcacgattg cctgctttgc catgctttca tagctcggta tcatggaaag
    18241 taaaaagctg ataggcagaa acatagcaaa gataataaaa agtatctggc tgaataacat
    18301 catgcccgta agcaagaata caaatatcgt tatccctaag ttgaagaaca gtaggaagaa
    18361 caccatacct aaacggttca cgacctgcgg tatcgtcaga ttgttgttgt cgttgtcctc
    18421 gatttctgtt ttcacgactt cctctctggt ctttccgtcc tcgtcctctg ggcttgccga
    18481 taccagagct tctacacggt ctgccccgat ttcttctgcg ttgctgttcc cgaattgtaa
    18541 gagtaaccac ggctgttcca cttgaataga aaataggctg tcccttatca agtccacgct
    18601 gtccttgccc ttgctgtcag agtttgggag catgattttt gttcccaagt ccaacgaagc
    18661 ggtactaata tcacttgaaa attcatttat ctttttgatg tagtcgggag cgtaggcaat
    18721 aaacgaagcg gacagcacga acaccaccac aaagttaata acggcatgaa gtgccttgct
    18781 ggtttcccgt tttatcagtc ccgtataggc aacataaagt cccaccacta agataatgag
    18841 aagcaggaaa ccaacataga agcccgtaga agaaaaaccg ttctgtgtta cgcctgcaat
    18901 ggtctgtatg ctttttccga tactgtctgc catatcgtta atgaagtcca gtttataggc
    18961 ttcctgcacc acatagcccg tggcattgct taaataaagg cttatcgtcc agacaaagtt
    19021 ggtaatgcag taaagcccat actgcaccga ttttccgata ccgtccagcc agttccacgg
    19081 aagccacgac cagctattat ccacataaaa atcaagctgg taattcgaca gcggatattt
    19141 tgagtaaaga ttttctgcat ttatggtatc gtccacaagc cccgtcgcat gagccaccgt
    19201 ccccagaagt gaaagcagga taagggagag tgccaccacg aacagaacca ttttgagaaa
    19261 gtggaaaatc ttcttttttg tgaacgcacc ttttatcctt tctttcatca ctccacctca
    19321 tttctctgta cgggcggtct ggtatcaaag gcgtgtagca gttcttcaaa gacggggtgt
    19381 atctgcacca caccgacacg tccgtataag tcctgcaata agcattgtcc gttctctaaa
    19441 tcacgcaagc gtttctggtt gttctcgtcg tccttgtcga taccaaaaaa ctctaaggtc
    19501 tgttttatct cgttaatgtc agtagaacga aaggcaaact tcaagccgat attgtttttc
    19561 agactttcct ttgaaacatc ataggcagac tgggtaacaa aataaacgcc tgcctgcata
    19621 gctcgtccag cacgaaccag cttgttagag agcgtttctc cttgtgccac atttaagaac
    19681 gcccacgctt cgtcaaggtc tacaatctta aaaatacttc tgtccgaatg gataaaatca
    19741 agggcaaagg tactaatcac aatcagcata gaaaccgata ataattcaat ggtcgtgtat
    19801 tcctcaaaag tggtatcttt atctggcagt acaaggtcag ctacttgaat gatgttgagc
    19861 tggttatcca gactgatagc attttctacc gtcccgtctg aaaacagcag atgtgcaaag
    19921 tcgtagtccg tgaagctgtc gatatggtct gcgatatttc ttgatatggg cgtatcttca
    19981 cggcgtagct cgtctatcac atggagcaag ccccgactgt tgctctgggt aacggagcgt
    20041 accgctttac gaagtacggg gaatttttcg ccgtccctag aggaaatacc cgtaaggaat
    20101 gttaagatgt cgattgccag actttcagcg tcttttacat tcttcataat cacgaatggg
    20161 tcaagaagtc ctgcatttcc tttatcgctg gttaagttta cgatattgat ttcatgtgcg
    20221 atttctggaa gtgtttcctt ccagttgcca cgctctgatt ttgggtctaa gataaccgcc
    20281 tgtcccccaa agagaaccga ataatacacc agaagattgt tacagaacga ctttccaccg
    20341 ccaagcgaac cgacaaaagc agacgcaagg gcgttggtaa ctgtaccttt tataccttga
    20401 ctggcaaggg acggttgcag gtacacattt cttcccgtat caacggaata gcccatatag
    20461 atacccgtag tttctcctaa ctgctgtgtc gcaccaaagc caagcccagc taaaaaatct
    20521 gattttacat actgcacata atcattgata tatcgcttgc tggcaggtaa aaattcagaa
    20581 tgaagcccca gcatatcccc agcaggacgc accagcttta cattgaggtc gtcgtaaaag
    20641 tccttgactt catcacaacg gcgttttaat tcgtccaaat catcagccga tacacgcact
    20701 acataagaga gcttatacat actttctttt gtctggtcta agtccgtttc cagctcatcc
    20761 acgctgtcta atgcgtccac cacatttgaa ctggtttcac ttcctgcttg ataggcgtga
    20821 ttatccaaat ctttcaattc ctttttctta tttcttactg tggtaagagc ctttcgattc
    20881 tccacgattt ctacattcat agaagtatca acggggaatg tgaattgctg ttgttggaaa
    20941 tagaagattt cacttgacgg aaaatcaagc tccccgacaa tcgcattgac ggtaaagtag
    21001 gacacatagc tttccttgtc ctcatgttcc aatcgtaaat accgctggct ttcctcaatc
    21061 acacaccttg tcggacggat aaggtcgtag tattttatca gcgtttcttt gttcaatttc
    21121 ttctttggta actgatactc ataatcttca taggcgatac cgttcctgcc gtaaagatgt
    21181 tccatgagat acccaaaatc attgatttcc aagcgacgca ccttgaagcg acgggagatt
    21241 ttattttcca gtaacttttc cattttcatg taacggttga tttcatcatt cggcatggaa
    21301 acaaaatcat tcatcagcgt gtggttcact tcatggagaa attccgtgaa cgtcagccac
    21361 gccgattttt ttatgttctt cagattgagc tgttcttccg taaccatgag cttaaagcca
    21421 agaaaaaagc ggtagtccac ttgattgtca ccaatcatag atactaacgc ttcggtctgt
    21481 tcgtctattt tctggcaggc aacttccttt aattttccag ttaccagctt ctttgactgc
    21541 tcctgcatac tatggattga gctttccgta gcaatctgca acgcatgaat tttaccctca
    21601 cgggactgtg cgataagctg tcggaaactg tcatgcacga taaatttctg ctctgcggat
    21661 agaaaagaat agttgtacgg tatcagctca tagtaagcga acacctcatt gtccttgttc
    21721 cagacaaggt tattgtcaat atattttatc gggaacatag ttcacactcc ttactgccgt
    21781 gattgtttca ttcaaggtct gcttatgcag ttttacggct tttcccgcat aagtgatttt
    21841 aggtcgcagg gcataggtta tctgtgattt caaaaagctg tacggcttct ttccgtcaaa
    21901 ggttttctgc gacataaacc aagtgagagc aacgggaata ccgaagtatt tgagaaatgc
    21961 tccctcaatc atggaaagtg ggggaatatc cccaaacaga atgatgataa attccgtaat
    22021 cacaaaccat gtaatctgtg taaaggtaac gggaaaaggc aggttaaagt cattgattgc
    22081 atacaagact ttttccacgt tccagatacc cgtataagat ttaatctttt tcaagttctt
    22141 tcagctcctt tcgtaagttc ttcaaagaga aaaggacagc cattttcaga ctgtccctta
    22201 aaagagaaat acgctgtcag tagcaaccat gccgttgctg atcttccagc gttaatatgg
    22261 tatctcgcat atccctcggc ttgtttcaat aaagcaaccg cccatatcca aatctcgccc
    22321 gtaggcttca taatcaatgt agttctgtaa gctgggtgga atgtccccaa gtgcctgcaa
    22381 ttcgtctatg tagtaatagg caacatctgt catggtttca cagtcgggat aataataaat
    22441 atcgtccttg tgttccacga cttcttccag cgtcccgtag tgactgataa attcgtccag
    22501 acactctacg atatagtcgg gaagttcctc tatcatttca tacatctcat tgagttcttc
    22561 aatggaaaca tactcgccaa tcgcaatggg gaagttgtcg gtatcatgga tagcgtattc
    22621 ctcatactgt tcattcaagc cgattttttc ttttacatct tcctcgtcaa tggggaacgt
    22681 gaaccaagca ccgactaaat aaccctcatt gtatttaccg agattagcaa tgtaaaccgc
    22741 catatcatct atcataagca ccacctcatt tcctactctg gtagttcaaa gataccgtgt
    22801 tccgttacta agaatttccc gtgttcctgc aagtgagaag cgtaggcttc aaagtcaaag
    22861 tagtattctt gacagtcctc ggataaatgt ttgaacgtcg agtcattcat cagcttttgc
    22921 cttgcaacat ctatcatgct tttgcagtcg ggataaaccg taatcacgtt cctgcaaata
    22981 taaagtgctt ctaaattctc aaacaccgta agtagtgata catattcttc ctgcatatcg
    23041 cttgaaagct ggcggtacat aaactctaat tcgttaagct ggtacacgct tgtatgttcg
    23101 tggacttcat cagcataagg caacaccttt tcgataatgc ggtacgccgt gctttctgca
    23161 ccgacaccta acttttcttc aaactcggca acatctatcg gcaggtcgaa ccagtatgat
    23221 gttgtttctt cgccagttgt ttctctcgtt tccaccagca cccttgtttc ctgcattgtt
    23281 cttcacgtcc tttctgttgt ttcatcacat cttttaaggt ctggtacggc ataccaaaca
    23341 caaactttga aaaatcttct aattgtcttt cttcataatc ggcagggctc agccgtttca
    23401 tttcacgcca caccttacgc ttgtaagagg gcaggtcaga aatgtattca cagccgactt
    23461 ttgcctgcat atccttaaaa atatcgttca tgtaatcact ccaatctgaa attttggtat
    23521 gaaaaaagca gtcaatcata agactaactg ctttgtgtga atattaaatt atagcttatc
    23581 ctaatctctt tttaatcttt ctttttagtt tgaaaaagaa actcgttgaa tattgctcgt
    23641 ttttcactaa tagttcatag gtttccataa cattgattgt atatacaaaa cccaacattc
    23701 caacagcaag accgaaaaat gcaaaggaat gatattctgt ccatgaaata tagcctttca
    23761 cagtaaatag aaagaaaatt agtcctgcta taaagagaac agtacaagga acagcaacta
    23821 ttacatattt tttctttttt gaagtgtatt cgcttttcaa ttcttttaga tattcataat
    23881 caagtagtaa aggttcttgc cttaaaatgt tgtattcttg tttttcataa aaaattgcta
    23941 cgacaaaaaa tataattcct gccacaaagc aaaatgccat acctaaataa cgccaagtca
    24001 tattatctaa aaacaaggta tatggtattc cagcaaaaat gcaaagcata acccctaaag
    24061 caagataaag tcctaatttt ttctgattag cgataaaccc taccgacatt tccttgctta
    24121 cataatatcc tcgttcttca ctattcttta cattcttttc gtctttcaaa agataatccg
    24181 tagatacttc aaaaatgttt gataattgta aaagcttttc tgtttctgga aacccttgat
    24241 tgttttccca tttactaatt gcctgcctag tagtgcctat ttgttcagct agtgcttctt
    24301 gcgatattcc tttttctttt cttaacttga agattttttc tcctaaactc attgtgtgat
    24361 acctcctatg atttgatgaa taaattttat caagccataa tgttatattc tatatttttt
    24421 gaaatgcaca ttagcaactt caagttgcaa ccttgtataa tatacaaaat tcatagattt
    24481 ttctggtaag tacagcgttt tatgtaatac aacattcaaa ttataacatt cgcatatcag
    24541 taccacaata aaattttatg caccgataat cttattgaac agctctaaca atacatcttt
    24601 cacaccgcca gcattgaata ccaaaccgac agcaatcaag gcaactacca agaagccgat
    24661 aagtttggaa aactcacgct tgaaccctaa gtagataccg ataaccacaa tcgccataag
    24721 cactaagctc tgtgcgttac ttaaaaacca ttgatacaaa ttttgtccga aattcattta
    24781 attgtcctcg ctttcttcta attgtttcat tttatagtca gcttctttgc cgacattcaa
    24841 aatacacatc aagactatgc cgatacccat tcccatagat accagcagga agtcttttaa
    24901 taatacccac atttttcatc actcctaaat ttctactaaa tcttttgcag gggtcgtctg
    24961 ctgtttgatt atcatttcat gcttttctgt gagctttgcc tgctgttcga ttgtctccat
    25021 gtaatctgta ccgtttccct tatcaatctt tttcagcatt ttcaaggtcg gtgctacctg
    25081 cctttgtacc caacgcaatg tacgatctaa ggtataaggt tctggctttg tcgtcagttt
    25141 cagactttgc cgattatcgc cgataaacca agcccagcgg tcattgagtt tccagtcatt
    25201 tttccgcttg tctggttctt catcaacaaa ccgtacatac tgattgatga tagaaaaggc
    25261 ggtctgctct gcgtcgtaat acgtcagcaa atcccgtact gcataatagg cacgttcatt
    25321 tctaagccgt atctcaaaac ggttgataat atcggcttct tccagaggtg tccctaactt
    25381 gacatactgc tcatagtcct tttcatagat acagaaatat acatcagatt tcagcgaacc
    25441 aagataaagg gtacgtccca tatattctct gtcgtcctcg ctgtgcttga taagctcgcc
    25501 cgactggtaa aacttataac ttctggactt tccgatatat tcccgttttc tgcatttttc
    25561 cgcaagctct ggaatatcca aaatgcccgt atggtcgttg atagcaaggt cgatacgctt
    25621 catcacgcca ccgtctatga gtgcgtccat gagaaagtca taccagcttc tttgctgtgc
    25681 cagcaggtaa ctctcaaact gtcggcaacc acgccccttt aactctaaaa ggacaccttt
    25741 ttcttcgtca gccgacgtat agataaagat gtcccctaaa gaataatgct ccgtataact
    25801 gtaatgtccg taatcttcat ggagcatata gttgatattc agttttaata tatctttgat
    25861 gatgtgctgt atatccagcg tgggaaaacg aattttcaca taatcaaaca gcatggtaag
    25921 cggtgcttct ggattgaacc ttgccagttc tttatgcaga gtttccagaa gttcctttga
    25981 cggcttcatt tttccgcttt ctatcttgtt gagatattcc cttgtgatac cagaagccac
    26041 cgccagcctg ccttgtgaga taccgtaagc aatccgtttc tcccgtaatt cttttatcca
    26101 ttgttcttca ttcagaacca tacccccaat ctgtcaagtg tgaagttttt taaggcgact
    26161 tcacagccga tttttactgg gaaatcatgc cagaagcctt atgtttccta tgtttttcgt
    26221 ctattgtcta tttgcaaacg tacccctctg ttagataccg aggggtttta cctgctggcg
    26281 tggcttacgc cacaccagca acggctagtc cgtgccgtcg cctttcgctt cgcacgtcgc
    26341 cgtcccgtcc tgccttgctt gtgcaagaga gccgatagtc tgcaaaaagt catgtccttt
    26401 cgggactaaa ggcgtgtaaa attcgctgat aacgcttgtt cccacatcac aatagccacg
    26461 ccccttgata cgcttctgga aaaactgctt tttcacatct gaaccaaaca gcataccgta
    26521 acctaattcg ctgatacgcc caagtcccac gcggaaattg aagttatctc tgataccgtc
    26581 cgagaaatac tttgcgtctg gacgctggca ggcaacgata aggaaatatc ctgcttgccg
    26641 tcctaacata acgattttct ttaactggct aagtaagccc acgctttctt ttgtccccag
    26701 catttcaaaa aacgccacat attcatcaaa gataagaaag cagggtggca gtcccagata
    26761 agcgtagttt tcgcccgtct tatagttcgg gtgtcgcttc atttcctcac ttcgctgtac
    26821 cataccctca taaaaggcat tgacgcaatc aatcattttt tctttggtgt gatacacatt
    26881 ttccataact gtccctaagt ctgctaagtc cgcatttttc gggtctaaga tgtaaaggac
    26941 agcgttggta tgcagtaagg cttcaatgag cgtcagcaga aaataggttt taccgccacc
    27001 agtcccacca gcaatcagag cgtgagggag tgcgtcatat tcccagacaa gatttttcat
    27061 caatttaaga cagccatttt ctgcccgtac ttcatcaata gtaatgcggt tcgctatcat
    27121 atcataaagc agggtatatt cgatataacc gtcatgtaag gtcttgtcgg tcagctcaca
    27181 atataatcca ctttccaatt tatcctctaa ccgtaaaagc tggtcttgat attttcccag
    27241 cgtaatttca cagcggatat gaagcagtcc cttttccatt tgataataaa tctttggaaa
    27301 ccagacgatt ttttcccttg atctgctttg caggtcagta aaaaaaccgc tgtcttgtac
    27361 ggtatcggct tcataccact tattttccag tatcattctc gccagctttt gacggtgcag
    27421 gagcttcttg aaactgtcgt aacagaagcg gtaatacaga aaagcgacca atgcacaaac
    27481 accagtcgct atcagtatgg ttatgaagtt gtaaggggaa agcgtcaagc cgttctctaa
    27541 caagctgaaa tgctcccagt cggtacgcat gagctgtttg atattcagta gcaggagaac
    27601 cgccacgaat aaaaacagaa gcgtccctat ggaaaaatgg tagacaaggt gcttgtcgct
    27661 ggctctgata cggtgtcctc tattccaaat cttacgcata aatcattcac tcctttctgg
    27721 gtacgaaaaa agcagtcaat catatagact aactgctttg taaatgttgc aagatataat
    27781 cataaatctg catttttagt tatattaagg tcgcacatat aatgccaatt acaccaatag
    27841 ccaaaaataa cagtccaa
//
LOCUS       DAEPLF010000028        27454 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEPLF010000028 DAEPLF010000000
VERSION     DAEPLF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.028"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            59..295
                     /locus_tag="KR314_001113"
     CDS             59..295
                     /locus_tag="KR314_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBH0327507.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            308..1651
                     /gene="yqfD"
                     /locus_tag="KR314_001114"
     CDS             308..1651
                     /gene="yqfD"
                     /locus_tag="KR314_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBH0327508.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            1590..2705
                     /locus_tag="KR314_001115"
     CDS             1590..2705
                     /locus_tag="KR314_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBH0327509.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKVKMQEKTFKRK"
     gene            2762..3223
                     /gene="ybeY"
                     /locus_tag="KR314_001116"
     CDS             2762..3223
                     /gene="ybeY"
                     /locus_tag="KR314_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBH0327510.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            3229..3948
                     /locus_tag="KR314_001117"
     CDS             3229..3948
                     /locus_tag="KR314_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBH0327511.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            4067..4468
                     /locus_tag="KR314_001118"
     CDS             4067..4468
                     /locus_tag="KR314_001118"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBH0327512.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            4532..5425
                     /gene="era"
                     /locus_tag="KR314_001119"
     CDS             4532..5425
                     /gene="era"
                     /locus_tag="KR314_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBH0327513.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            5437..6816
                     /gene="mgtE"
                     /locus_tag="KR314_001120"
     CDS             5437..6816
                     /gene="mgtE"
                     /locus_tag="KR314_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBH0327514.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            6889..7044
                     /locus_tag="KR314_001121"
     CDS             6889..7044
                     /locus_tag="KR314_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBH0327515.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            7010..7783
                     /gene="recO"
                     /locus_tag="KR314_001122"
     CDS             7010..7783
                     /gene="recO"
                     /locus_tag="KR314_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBH0327516.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            7776..8375
                     /locus_tag="KR314_001123"
     CDS             7776..8375
                     /locus_tag="KR314_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBH0327517.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            8535..9413
                     /gene="glyQ"
                     /locus_tag="KR314_001124"
     CDS             8535..9413
                     /gene="glyQ"
                     /locus_tag="KR314_001124"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBH0327518.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            9413..11479
                     /locus_tag="KR314_001125"
     CDS             9413..11479
                     /locus_tag="KR314_001125"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBH0327519.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIRLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(11645..13192)
                     /locus_tag="KR314_001126"
     CDS             complement(11645..13192)
                     /locus_tag="KR314_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBH0327520.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            13472..14239
                     /locus_tag="KR314_001127"
     CDS             13472..14239
                     /locus_tag="KR314_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBH0327521.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            14522..14719
                     /locus_tag="KR314_001128"
     CDS             14522..14719
                     /locus_tag="KR314_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0327522.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            14748..15824
                     /gene="vorB"
                     /locus_tag="KR314_001129"
     CDS             14748..15824
                     /gene="vorB"
                     /locus_tag="KR314_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBH0327523.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            15827..16546
                     /locus_tag="KR314_001130"
     CDS             15827..16546
                     /locus_tag="KR314_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBH0327524.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            16559..17098
                     /locus_tag="KR314_001131"
     CDS             16559..17098
                     /locus_tag="KR314_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBH0327525.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            17255..18313
                     /gene="buk"
                     /locus_tag="KR314_001132"
     CDS             17255..18313
                     /gene="buk"
                     /locus_tag="KR314_001132"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBH0327526.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            18331..19224
                     /locus_tag="KR314_001133"
     CDS             18331..19224
                     /locus_tag="KR314_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBH0327527.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            19254..20462
                     /locus_tag="KR314_001134"
     CDS             19254..20462
                     /locus_tag="KR314_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0327528.1"
                     /translation="MKYAKRMDMVKASAIRASQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDIVVTENPSYLGAINAFRPYECDFLGVDTDDEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            20486..21748
                     /locus_tag="KR314_001135"
     CDS             20486..21748
                     /locus_tag="KR314_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0327529.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFSTPVLGMRWVMISLGILSIVMCPVLWFVIKDVDIREDKEEEASEKMSAGDFM
                     TVLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFG
                     APFGGYCADKVKSPTKVLLPIYVFGIAVIILFLVLPASTPMTIFIALTFVVGILGYMG
                     KGIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIF
                     QIAILIIGILSCLYILKVKKKRESKLEA"
     gene            22324..23787
                     /locus_tag="KR314_001136"
     CDS             22324..23787
                     /locus_tag="KR314_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBH0327530.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSMKLRFFQIKDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSILFSVVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVTSSIALGIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIIANINLVPGAIMTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIIITGMYNVTDVYTTLPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKHIIN
                     VCRFMLIICAAMCCFIEPLTSWALGDICIGVLTYINLVGIFTLKKPAIKAYYDYQRQK
                     KMGVKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            23843..24979
                     /locus_tag="KR314_001137"
     CDS             23843..24979
                     /locus_tag="KR314_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0327531.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            25215..26330
                     /locus_tag="KR314_001138"
     CDS             25215..26330
                     /locus_tag="KR314_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBH0327532.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALTPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKAKKIGEKELMNLLSLKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            26482..27351
                     /locus_tag="KR314_001139"
     CDS             26482..27351
                     /locus_tag="KR314_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0327533.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT        10699 a         3001 c         5139 g         8615 t
ORIGIN      
        1 tcaataaagt gcataaatac ctatttcaag cataaaatta attaaggggg taatgcttat
       61 gttagaaata tccactgatt tacaagtaaa tcaaccagtt attactgtta caagtaatac
      121 gtttattagt atagaaaatt atctatcaat attagaatat gaagttgatt taataagaat
      181 aaagactaaa gttaaaacaa ttaaaatatc tggggataaa ctttcactta agtatattac
      241 tgatagtgag ataggtataa aagggattat ctacaatgtt gagtatgttg attaggagga
      301 gatacacttg ataagtttta taaggggata ctatgttata gttgtggaag gagtaggact
      361 cgaacaattt ttaaatcatc ttataagaaa tgggataagt gtttacaatg taactagaat
      421 aaaaaatact aaaatggagt ttcatataga tagacaagat ataaaagaat ttaagaatgt
      481 ttatagagga agtaagtttg atataaaggt aaagcaaaaa actggggtcc cttttattat
      541 caaaagagtt tataagcaca aaggtatgtg gatttgtgct cttgtttctt tatttttatt
      601 gatgtctaca tcgcagtttg tgacagatgt atatatacaa tctcctgaag gtataaaaaa
      661 agaagctctt agaaatgagc tttataaggt tggagtaaga ccaggagtct ataagaagag
      721 tattgataga aaagaagtta gagaccatat gatgtcaaaa tttaatgatg tggcatattt
      781 atctatcaat gtaaaaggaa caaatatatt tgttactgtt acaaaaaagg cagaatccct
      841 aaaatctaca gaccaatcaa attattgtaa tgtaattgct ttaaaaaatg gtataataga
      901 aaaggtaatc cctagaagtg gtaaatcagt tattaaatct ggagacatag tccaaaaggg
      961 agatgtctta ttaaatggag ctaataccaa gtcaatacca gaagtatggg caagtacttt
     1021 ttatgagtcc acaaaaaagg caagttatgt agatactgta aataaaaaaa ctggagaaaa
     1081 gaaaaatata tatacactaa gtttttatga taaagagttt acaatgagaa aaaatataaa
     1141 atataaagat tatgttgttg aaaataaaga aaaaaaacta tcaataggaa actatacttt
     1201 tccaataaag ataaaaacga gtacttttta tgaaacaaaa aaggttgaag taaaaaggaa
     1261 taaagaagag ttaaaaaaag aattatctga aaaagcttta aaagagttag aatacataat
     1321 cccagcttcg gctagaataa tagatgtaaa acataacttt aaggttaata aaaatatgtt
     1381 agaatattta ataacagttc aaactagtga aaatattgct aaaatttatc ctcttagtaa
     1441 gtcagaagca gaaaggttta taaaggaaga aagtaaacca gatgaaggtg aagaggaagt
     1501 tccttccaat ccagaaaaaa gaccattaaa tgatataaga aatgagtttg atgaggataa
     1561 taaggataat aaagaccaaa acaattctga tgaaaataat agtaatcaaa atagtaataa
     1621 tagtcaaaat aataatagca ataacaacta ataaaattaa acttaaaatc ttaggaggac
     1681 taattcaatt gataatacaa aagaaattta caatagcaga tggtaacttt gaaagagagt
     1741 tatttggcaa ctttgatgaa aatgtaaaat taatagagaa gactttaaat atagatgtaa
     1801 tacttagaga aggcaatata attttaattg gagaagaaaa aaatgtagat tctgcattaa
     1861 aacttatgaa tgaattacac caaactgtat caaatggtaa gcatttagat aagcagagta
     1921 tttcttattc attatctctt ttgcttgaag gaagtgaaca aaaaataaaa gaacttgagg
     1981 ggacaatcgt cataactcaa aggggaaaag ctgttcaacc taaaacttta ggtcaaaaag
     2041 aatatataaa acttatagaa aataatgata taacttttgg tgttggacct gctggaacag
     2101 gaaagactta tcttgcggtt gctatggcag taaaagcttt taaaagagat gaagttagta
     2161 gaattatact tacaagacca gctgtggaag caggagagag tctaggtttt ttaccaggag
     2221 atttaaagga caaagttgac ccatatttaa gaccactata tgatgcctta tttgaaatgt
     2281 tgggtgctga taaatttaat aagtatttag aaagaggtac tattgaggtt gcaccacttg
     2341 catttatgag aggtagaaca cttgataatt catttataat attggatgaa gctcaaaata
     2401 ctacttctga acaaatgaaa atgtttttaa ctagacttgg atttggctct aaagctgtag
     2461 ttacaggaga tgtaactcaa acagatttac cacaaaataa gaagagtggt ctaatacaag
     2521 ctacagagat ccttaaaggt gttccaggta ttgggtctat aatgctaaca gatagagatg
     2581 ttgtaagaca tgagttggta cagagaataa taagagctta tgagaagcac gacaaaagag
     2641 aagaatttaa aaaagaagaa cgtaaaaaag tgaaaatgca ggaaaagact ttcaaaagaa
     2701 agtaaaagtg tagtaatata gatatatagc attattaatg aataaaaagt aggtgtagtg
     2761 aatggatttg atactagatg atagacaaga taaattagaa gtaagtgagg agttaattga
     2821 aaagataaag gatataatca tagaatgttt agattatgaa ggatatgatg ataattatga
     2881 agtgagtctt tcttttgtag ataataaaga gattcatgaa ttaaatagag aatatagagg
     2941 agttgataga gtgactgatg ttttatcatt tcctctttta agtgatgatt ttgaggatgt
     3001 tgagttagaa gaagaatccc taggtgacat agtagtatct cttgaaagag ccttagaaca
     3061 gagtatagag tataaccata gttttgaaag agaagtatgt tttttaatat gtcatagtat
     3121 gtttcatctt ttaggttatg accacgatac agatgaaaat acaaaagaaa tgagagaaaa
     3181 agaagaacac atattaaata agttaaacat aacaagggag tagtttttat gaaaccagaa
     3241 aaaactcgtc aaggtataat taaagcattt aatgcagcta tagaagggat attgtataca
     3301 tttaaatttg aaagaaatat gaaaatacat tatttaggtt cagttgctgt tcttataata
     3361 agtttgtttt ttaacttttc aaaattagaa atgataatgt tacttatgtc catatgctta
     3421 gttgtagttg ccgaaatgtt taatacagct atagaaaaag ctgttgattt agtaactgat
     3481 gaatatcatg ttcttgctaa gatagcaaaa gatgttgcag ctggaggcgt tctagtggca
     3541 gcactaaatt cagtagtagt tggatatatt ttgttttatg ataaattgac agatatatct
     3601 ggaatattaa tttataaaat aagagaatca gaattacata taaccttgat atgtatatta
     3661 ttggttctta tagctgttgt agttgtaaaa gcattgactt ctacaggtac tcctctaaaa
     3721 ggaggaatgc caagtggtca tgctgcctta gcatttgcta tagcaactgc aattacactt
     3781 atgactgaaa gagttgttgc ctctactctt gcatatataa tggctgtatt agttgcccaa
     3841 agtagaattg aaggaaaaat acataccttt tgggagacta ttgctggagc tttattaggt
     3901 gtgttgatag ctatattagt atttcagctt ggaatgttct ataattaaaa taaatgtaca
     3961 taaaatattt aaaataaaac aaatctgtgc taattaaatt tttatgatat aatgtagagt
     4021 attaaataaa aataactttt aattattgta tacaggagga gttgttgtgg ataacataga
     4081 attgttaagg ttggcagaag atgccagaca acattcttat gctccttact caggttttag
     4141 agtaggtgca gctcttttaa ctaagagtgg taaagtttat actggatgta atatagaatg
     4201 tgcatcatta ggtggaacaa attgtgcaga aagaacagct atttttaaag caatatcaga
     4261 gggtgataag gatatctata aaatagctat agcaagtgat aactcagaaa ataatgagca
     4321 aacatatcca tgtggaatat gcaggcaggt aattatagag tttggttctg atataaaaat
     4381 aataacggga tatactaaag gagaggtgtt tgagcatagt ataaaggatt tgttgccaaa
     4441 ctatttctct ggaaatgatt taaaataaat ttaaaagtat aaataaattt aagtaattaa
     4501 aacaagaaga taaaaagtta ggagacagtt aatgttcaaa tcaggttttg tcagtatagt
     4561 gggtagacca aatgtaggaa aatctacttt aatgaataat gtagttggag aaaaaatagc
     4621 tataatgagt gataagcctc aaactacaag aaatacaata caagcagtat atacagatga
     4681 agaaatgcag atagtattct tagatacacc cggtattcac aaaccaaaaa ataaattagg
     4741 tgaatttatg gtaaaggcag caacagaagc ttttaaaaat gtagatttaa tattatttgt
     4801 agttgatgat tctaaaaaaa taggtcctgg agatagaaag ataatagagg atttaagaag
     4861 tgttaagaca ccaataattt tagttgtaaa caaaatagac caactagacc aaaaagatga
     4921 gctatttgat ataatcaaaa tgtatgatag agaaggtatt tttaaagaaa tagtaccaat
     4981 atcagcatta aaaggaaaga atacggatac cttaataaaa gttatacaaa attatttaga
     5041 agaagggcca aagtattttc cagattatat gattacagac cagccagaaa gagtattaat
     5101 agcagaactt ataagagaaa aagttcttca ctatcttaat gatgaaatac ctcatggtgt
     5161 agctgttgaa attgaaaaaa tgaaagcgag aaatgataag gaaatagtag atgtatctgc
     5221 tgtaatatac tgtgaaagag attcacataa gggaataata ataggaaaaa atggtaggaa
     5281 actaaaagga ataggtaagt cagcaagaca agatatagaa ttgcttttag gttcacaaat
     5341 aaatcttcaa ttatgggtta aagtcaaaga aaattggaga aatttacaaa attatataaa
     5401 taattttgga tataatgata aataaaggtg ggttatatgg ataggaaact ggatgcttcc
     5461 caagatttat tatatgaagt aaaatcatta attgataata ataaagtatt ggagttaaga
     5521 gaattaatag aggaatatca tataattgat atattcgata taatggaaaa tttagaggaa
     5581 gacatgaaaa ttcaattatt tgaagtactt cctttagata tggcatcttc tatattagaa
     5641 gaaggtagtg tagagttttt tattagtatt ttatctaaac tggatgtaga acactcgaaa
     5701 aatatacttg aacttatgtc tcttggagat atggcagaca agttgagtga acttgaagaa
     5761 gaagaaagag aacacataat aaatcttttg aatcaagaga atgccgatta tgtaaaagag
     5821 ttactattct atgatgaaga ttctgctggt ggaacaatga ccactggata tatttctata
     5881 aataaagata tgacagctct tgaggctata gaccatatga gagaagaagc tgaagaagct
     5941 gaaactattt attatatata tgtggttgat gatgaagaaa aactagttgg agtattgtca
     6001 ttaagagaat taataatagc tagagacgca aatatagtag aagatttgat gagtgaaaat
     6061 ataatctctg tttatgtaga tgaagataga gaagaagcag taagattagt atctaaatac
     6121 aatttaatag caattccagt agtagacaga caagaaaaat taaaaggaat aataacagta
     6181 gatgatataa ttgatgtaat ggaagaagaa gcaactgaag atatgtataa atttgcagga
     6241 agttcagaac atgaaagaga agttgctgaa aaagaaaacc ctacattgag agagcaaata
     6301 atatcagcac ttagaggacg attaccttgg ttaattatta ccttggttgg agggttgttg
     6361 gcttcgttaa tactttctaa cttagactat ataatgaatc ctgtatatgc tccattagta
     6421 ttttttatac ctgtagtaat tggtatgggt ggaaatattg gaactcaatc atcatctgtt
     6481 acagtaataa cattatctaa taaggattta aattttagca atgtggttag agaaggtatt
     6541 gtaggtatta ttacaggtct tttgtgtagt ataataacag gtattgtaat ttactttgtt
     6601 atgagaaagt tagacattgt attaattgtt tcaatatcat tatttataaa tatggtttta
     6661 ggtgcaacaa taggtgcgtt tatgcctgtt ttattaaaaa aaatggatgc agacccatct
     6721 acagtttcat caccaattat atctacagct cttgatataa ctggtatagc agtttacttt
     6781 ataataacaa cggcattatt gtcaaaaatt gtttaataac tttatgtatg atttatcctc
     6841 ttcttactaa aaatataagt ataaatgtaa gaagggggtt ttttaaaaat gaacaatatc
     6901 agttgggaag tttttaaaaa aacaggtagt atagatgcat atttatattt ctgtgattgc
     6961 aaaaacatac atgaagaaat tgaagaagta agggagaaaa aagaagacga tgataatcct
     7021 gaacacccag gggatagtac ttaaagctat aaggtataag gaaagtgaca taatacttac
     7081 actatttaca agaaagcttg gaaaagtatc tgcaatagca aaaggagcta aaaaaaataa
     7141 gagctctttg ctttcatcat cacaattatt ttcttatagc aattttacct taaaaaaaca
     7201 aggtaatatg tacaaggtta ctcaaagtga aataataaaa agtttttata atatatccta
     7261 tgatatagag gcattttctt atgctacata tattacaaaa ttagttgaaa attctatact
     7321 tgaaaatcag actaataata ggctatttat attacttgcg cagactctgt atttgtatac
     7381 acaggataat acagacaata ggtttataac agcagcattc gagttaaaat ttttagacta
     7441 tataggattt aaacctatag taaataaatg cattaattgt gaatgtaaaa ttttacaaaa
     7501 ttctgtgttt aatatctacg aaggtggtat actatgtagt aagtgtagta aactttttga
     7561 taataatata aaattagact taacaactat aagtcttatg gaatatgtat taagaaatga
     7621 tattttaaca tgtagtaaag ctaaagtatc aaaatatata acacatgaac ttgagaatat
     7681 tctagacaag tatttgaaag tttatgttga taatattaat tttaaatcat tacacgtatt
     7741 gcaaagtgta aaaaataata agggagtgga taatgatgag tagtaatata actattgaaa
     7801 tggttgattc agtctttgaa agagttccag gagcaactta tgcagaagca aaagaagcat
     7861 tagtaatgtg caatggagat gtaattgagg cagtaatata tttagaaaat aaaaaaaata
     7921 cttgtaattg taaagcaaaa tctgctaaag aaactatgga agaagtattt ggcaaagatg
     7981 gagaaaatat aaagtttcaa ttaaaagagc ttcttagaaa aagtagtgta gtgagaataa
     8041 tagttgaaaa agatggaaaa actatgatga acattccttt aacagttgga gttgttggat
     8101 tagcttttgt accattagct actttagttg gattatcagc tgctgttata agtaaatatc
     8161 gtataaaagt tcaaaatgaa gaagatggag aaattgtaga tttaggtgaa ttgaatgaag
     8221 aaaaacttca cgttttgaag aatatgataa ctaatgcagc aaaagatgtg aaggatgtag
     8281 tagttgacaa aaaagaagat gacaaagatg taactgctga ccttatgaaa gaagaatctg
     8341 aaaagaataa agaagacgaa gacgaaaata aatagatata aagtgacttt taaaaaatga
     8401 agagaagata attataagaa ttatcactaa cattgacaaa ttttatagaa tttgatattc
     8461 taaaagttgt agaagaacta ttaaaatagc ctttcgtgta gacgagaggc tttattgtta
     8521 taggagggat ttttatgaat tttcaagaga tgatactggc gttgcaaaaa tactggtcaa
     8581 agcaaggttg tatcatgatg caaccttatg atattgaaaa aggtgcagga actatgaatc
     8641 ctaatacttt tttaagatca ttaggacctg agccttggca agtatgctat gtagagcctt
     8701 caagaagacc agcagatgga agatatggtg aaaacccaaa cagactatat caacatcatc
     8761 aattccaagt tatactaaaa ccttctccag ataatataca agaattatat ttggaaagtt
     8821 taaaagagat tggaattgac ccaagtgagc atgacataag atttgttgaa gataactggg
     8881 aagctgctac agttggtgct tggggacttg gatgggaagt ttggctagat ggtatggaaa
     8941 taactcaatt tacgtatttt caacaagtag gtaatattga atgtgagctt gagacaggag
     9001 aaattacata tggattagaa agattagcta tgtacattca agaagttgat agtgtttatg
     9061 atttaaaatg gaatgataaa ataacttatg gtgaagtatt taataaagct gagtatgaaa
     9121 actcaatgta cgcatttgaa ttatgtgatg cagatatgtt gtttaatctg tttgatatat
     9181 atgaaaaaga agcattaaga cttatggaaa atggccttgt aataccttca tatgactatg
     9241 tgttaaaatg ctctcatgca tttaatacat tggacgcaag aggtgcaata ggagttagtc
     9301 aaagagcttc atttataggt agagttagaa atatggcaaa gactgttgca gaaacttttg
     9361 taaaacaaag agaagaaatg ggattccctc ttttaaagga cggtgacaaa taatgaaaaa
     9421 ttatctttta tttgaaatcg gtgttgaaga aatgccatca agatttgtag gcagtacact
     9481 agaacaatta aaaaacaatc ttagtaaatt atttgatgaa aatagaatag cttttgataa
     9541 aataaatgcg tatgggacac caagaagact tactttagta gtcgaaggat tgagtgatag
     9601 acaaagtgat ttagaagagg aaataaaggg tcctgctaag aaaattgctg ttgatgcaga
     9661 aaataattta acaaaaccag cattaggatt tataaaaagc aaaggattaa gcgaatctga
     9721 catatatttt aaacaagttg gaaaagaaga atatatattt gcgactataa agcaagatgg
     9781 aaaagaaact tcagaagtac taaaagacat attaccagag actataaaat ctgtagtatt
     9841 cccaaaagct atgcgttggg gaggaaaaaa tcttaaattt gcaagaccta taagatggat
     9901 agttgcattg ttaaatgata aagtattaga gtttgattta gaaggaataa tatcttctaa
     9961 tgtaacaaaa ggtcatagat ttttaggtga aagtacaata gaagttgatt ctatagaaga
    10021 ttattttgaa aaattagaaa aaaattatat agttttagac caaaacaaga gaaaagaaat
    10081 gataagaaaa caatgtatag aagtagcaaa ttctttaggt ggagaagttg aatttgatga
    10141 agatttactt gaagaggtaa cttatttagt tgaatatcca actgcttttt atggagaatt
    10201 tgatgtggat tatataaaac taccaaaaga agttgttata actcctatga aacaacatca
    10261 aagatatttt ccagtactta aagaaggaaa actgttacct aactttatag ctgttagaaa
    10321 tggtgattct tacagaatag acaatgttaa agctggaaat gaaaaagttt tagaagcaag
    10381 acttgcagat gctttatttt tctatagaga agatactaag agaaacttag aaagctacat
    10441 agaaaaatta aaaactgttg tattccaagt aaaactagga actatttacg acaaaacttt
    10501 aagacttgaa aaattaagtg ctgatatact tgatagttta ggtttagtag atgaaaaggc
    10561 agatactata agggctgcta aattatctaa ggcagattta gttactggaa tggtaggaga
    10621 atttgatgaa ttacaaggtt ttatgggaaa agaatacgca aaagttggag gagagaatga
    10681 tgttgtaagt gaagctattt ttgaacatta tcttccaaga tttgcaggag atatacttcc
    10741 taagactaat gcaggggttg ccttatcaat agctgataag ttggattcaa tagcaggttt
    10801 ctttgctata ggaatacaac caacaggttc tcaagaccca tatgctttaa gaagacaagc
    10861 tttaggtata cttagtatac ttatggacag aaaagttgct gtggatataa aagtacttat
    10921 tgaacatgct ttagataatt attctgaatt agattttaat aaagaagaag ttgttgagca
    10981 aataatgaat ttcttcaatg agagagtaaa aaatctattt aaagatttag gaataagata
    11041 tgatgttgtt gatgctgttt taagttctga tataaatgat gtttctgata tgcatatgag
    11101 agctttagag cttaataatt ggctagaaaa agatgaatta gtggagatgc ttactgcatt
    11161 taatagagtt tctactctag ctcaaaaagc agaaagtgat aaaattgatg aatctttatt
    11221 aaaagaagat gctgaaatta gactttacaa tgaatttaaa caagtaaagg taaaagttaa
    11281 tgaattatta aaggataaga aatatggtat tgctttagat gaatttgcat ctttaagacc
    11341 agtagttgat aatttatttg atacagttat ggttatggat aatgatgaag ctattaagaa
    11401 caatagactt gctttactta aacaagtata tgatactatg ctagaaattt gtgatttatc
    11461 taaaattgtt tataaataaa atttaaacta tttataagaa ataaggctgt ctcaaaatag
    11521 agatagtctt atttttcatt gttgagatga tgaaaatata tttgatttca tagaaaaaga
    11581 gtgtctcata aactaaaaag ttcattttga gacacccatg ctttttgtaa gtaaatataa
    11641 taaattatat tttatactta tcagttattt tgtttaaaat attatcattt gaatttaccc
    11701 attcataaaa attgcctgat gatgcgtccg atattttata aagcatttca ggaatatcac
    11761 tagctttaaa atctccaagt gattttccca atctagtctt acctatttca aaactgcctc
    11821 caacaaataa ttcaaatgca tctgtagata taccatcaac attcttcttt ttaccacata
    11881 aacctatgct accaacttga tgaacaccac atgaatttaa acatcctgat atatataact
    11941 taggtgcttt atttaaaatt tcttcattat tttgtaatct aaaataatct atgatttcat
    12001 gtaacatttt ctgactattt tgtatgccca tttgacaaat tggaacacct atgcaagata
    12061 tactttgttc taattgagaa ttacaactaa tagttttact tatttctaat attctttttg
    12121 cttcatttcc atttaaattt aaaatatata atccctctgt cattcctagt ctaatcatag
    12181 gattttcaac attatctaat tctttaagta aagtacttaa atcctttgtt aacaaaatac
    12241 ctccaactgg atgtatatat acagtgtata aaccatcctg tttttgaggt attagtctag
    12301 aatcttgtat atctatttta actccaggtt tagagtaatc tatttcttca atattgagtt
    12361 caagactacc attttctttt tctaatttat agtattcttt aaatttttct aagaatagtt
    12421 cttcaccaag tgattcgacc atatatctaa ccctagctct gtttttattt tcataatttc
    12481 cataatccat aaagaattta atcatacctt caacacaata taaggcgtct ttcggcttta
    12541 taagttcatc aagttctaaa gcaactttgg gttgtttacc tagacctcca cctaagaaaa
    12601 ctctgaaata tggttcatca tttttcaaag tagcaacaaa tcctaagtct tgtatactac
    12661 aatgagcagt atctgtgtaa caattagaat aagaaacttt taattttcta ggtaaatggt
    12721 atgtagtagc atttttgata aaatatttat cagtagcaac tgcatatgga gtaacatcaa
    12781 agacttcact agggtcaact ccagataatg gagataatcc gacatttcta ggataattac
    12841 caccaccacc tctagtgaat atatcatttt ttattccttc ttccatgata tcaacaattg
    12901 aatttataga taaatcgtgt aattgtatag cttgtctagt tgttaagtgt atcttatcta
    12961 agttgtgttt aatagctatt tcatagattt tattaagttg atactgagat aaaactccag
    13021 cagatactct aagtcttatc ataaaagatt ttttgtctct ttgagcataa acaccatatc
    13081 caccagaaaa tcccttatat tgcattttat ttatttctcc atttaaaaat tttgaagaat
    13141 attctttaaa attaggtagt tcatttaaca atatcttttt aaaattatcc attatttcac
    13201 ctcattgtta gtcttgtata aaaatatatc tattgtataa tagatatata tattattatc
    13261 tagtcaggat aaaaatataa cagaataatt ctattaaaag taagaataat aaggacatag
    13321 tgagtatttt gtttttattt tacaaattaa gtataatatt tttgttttat aaaatataat
    13381 taagataaat agataatttt gtaattttaa tatataatat ttagtagagt gtgggagtag
    13441 cacttaatat ttgcaggagg agtttttgta tatgggagaa ataataaatg cattagatag
    13501 agccctagac ataattttgc tgttatatca tgaaaagaga gaaatgggaa taactgaaat
    13561 aagtaaggct atgggagtat ataaaagtac tgtacataga actttggtta ctcttgaaaa
    13621 caaaggtttt gttatacaaa atgcagagaa tggtaagtat tggctaggaa taaatttata
    13681 tgcaataggt atggttgtgg gagaaaaaat gtcattgact gaaatagtaa aaccatatac
    13741 gaaaaaatta aatcaagaat ttaacgaagt agttaatgta tctatattag aagaaagagc
    13801 tcaagattct ccaagaagta tcataataca taaagagtat ggttcaaatc aattactttc
    13861 agtaaatcca tccgttggtt caagtagtga atgttattgt tcagcagttg gtaaatgtct
    13921 tatggcattt aatgattcta tagattttga aaaatataga aaaacaccaa tacacaagta
    13981 tacagaacat acaattgata attgggatga tatgatgctt tttttagcaa aaataaaaga
    14041 acaaggatat gctatagatg atgaagaact agaacatgga ctaacttgta ttggagcacc
    14101 tatcttagat aaaaataata aagctattgc agccataagt ttgtcaggtc caactataag
    14161 aatgagagaa ggggactttg agtataagat taaaagagtt atagaaactg caaaaagtat
    14221 atctgaactt tttagatgat ttttaatctc gataataaaa ataggctata tttaaattgt
    14281 tatttgtaaa tatcagttta aatgtagcct atttttcttt aatctaaaat taaatattct
    14341 taaaatttaa aatgttattg aaatatataa gcgaatgtgt tatcttttaa ttaagcaata
    14401 aaaatagaac aaggttctat actatagaac aaaattgatg catatatagt atttatacaa
    14461 aatataaata ttatatattt aaaattaagt aaaaatatta cataaagggg agatttgaga
    14521 aatggaaaaa gtcaagttga gtgttgaaag atgtaaggga tgctatcttt gtattgaagc
    14581 ttgcaaaaaa gatgcaataa gtctttctga ctacacaaat aaaaaaggct ttgtaactat
    14641 taaagtcgat gaagaaaaat gtattcaatg tggcgcttgt tatacaatgt gccctgattt
    14701 agtatttgag atattataaa ttatttttat tagaggggag aaaaggtatg caaagtttat
    14761 taattaaagg aaatgaagct ttagcagaag gtgctatacg tggtggctgc agattttatg
    14821 caggttatcc aataacacca caatcagaaa tattagaaat gatggcatgg agacaaaaag
    14881 aagttggagg agtatttatc caaggtgaat cagaaatagc cagtgtaaat atggttatgg
    14941 gagcaagtgc acttggagca agagctatga cttcttcatc tggtccagga ttttcattaa
    15001 aacaagaagg tattgcatat tgggtatcag ctggtttgcc agcagttgta gcatgtgtgc
    15061 aaagatatgg tataggtgat ggatttatag gagctggtca agataattat tggcaagcag
    15121 taaaaggcgg tggaaatgga gattatcatt taatcgtgtt agcgcctaac agtgttcaag
    15181 aaaatatgga tatggcatat gaatcttttg aattggctga aaaatatatg catccagtac
    15241 ttatactttc tgatgctaca ataggtcaaa tggtagaacc ttgtatagca ccaccaatga
    15301 aagaatacga tatggataaa gctccatggg ctgcaaaagg aacacctgta ggagaaaaaa
    15361 ataaagtaat aactgatata acatattttg aagaccaaga tacatggcaa aaagggtata
    15421 tagaaaaaat gagaaaagtt caagaagatg aacaaagatg ggaagagata gaaatagaag
    15481 atgctgaagt ggtatttgtg gcatacggta tttcatcaag aatagcagaa ggtgcagtta
    15541 gagatgctag agcaaaagga atgaaattag gtctaataag accaaaaaca ttgtggcctt
    15601 acccaagaaa agcatttaag aaattaagtg attcattgaa gggacttgta acagttgaaa
    15661 tgaacatgat ggcacagatg aaggaagatg taattatagc atgtgataat agatttccag
    15721 tatattcagt agcaacagct caatatatgg cagaagtaaa tcaattaata gattttgctc
    15781 aacagattat agatggaaaa gctaagcaag aggaggtatt ttaattatgg ctaagaaagc
    15841 accagcaata atgatgtgtg aaaatatgtt ttgtggaggc tgtggacact ctatagtcga
    15901 tagaattatt ggagaagtat taacagaaat ggatatatgt caagatacaa ttatatgttc
    15961 agatataggt tgtaatcata tgtttcaatt tagtatgaat gtagatgttg tcgttccacc
    16021 tcatggtaaa atgggtgcag caatggcagc tatgaaaaga gttagaccag ataaatatat
    16081 atttacaata gctggagatg gtggagctta tgcgataggg cttggggaaa ctatgtcagc
    16141 tgctactaga aatgataatg ttatattcct tgttatgaac aataatatgt ttgcaatgac
    16201 aggagggcaa atggctccaa ctactataga aggtcaatat acagcttcta ctccagcggg
    16261 tagaaattac gcagatcatg gttcaacatt tgatgttgta aaagttatgg gaaatttaga
    16321 tatagcatat ctagcaagag gaacaataac taaccctaaa gaaataaata caacaaaaag
    16381 atacatcaaa aaagctctag aaaaacaaaa agaaaagaaa ggtttttgct taatagaagt
    16441 gcttgtacca tgtccaacaa actggggatt aaagccagta gatgctatga agagaattga
    16501 aaatgaactt gttccaaagt atccacttgg agaatttatt gattagggag gaaataatat
    16561 gttgaatgaa attatatgtg ctggttttgg aggacaggga gttcttacag caggaaaaat
    16621 tataatgtat gttgcatata aggcagaaaa gaaaattaca tggttcccat cttatggaaa
    16681 tgagatgagg ggaggaagtg caaattgcaa tgttgttgta agtgatgaaa aaattgcaaa
    16741 tccttgtgta aaaaatccag atatactatt gggaatgaac agcgtatcag tagataagta
    16801 tgaaagttta atgaaaaaaa atggttatat gtttgtcaat gaatcactag taagtaagga
    16861 tagaaagtac agagaagaca ttaatgttat aaaagtacct gttactgaaa tagcacaaaa
    16921 tttaggcaat gaaaaagctg ctaacatttg catgttaggt gctatggtaa agagtttgga
    16981 tttatttaca aaagaagaat ttgaaagtgg tatgtgtgaa tactttgaaa atcaaggcaa
    17041 aggtaaattc aattctaaaa atgtagaagc atttgaagca ggatacgact acgaatgaat
    17101 attttaaata tttaaaataa ttattgaaat acttattttt atatgttagt atttaattaa
    17161 taaaatatag aacgtcgttc tggacaatag gacgaagtct ttaggccgga atacaagaac
    17221 gacgttctgt atagtagaac tagggggtgt taaattgaaa gttttagtaa taaatcctgg
    17281 tggtacatcc acaaaaattt ctgttttcca agatgaaaat gaaattttga aaaaaaatat
    17341 aacccataca agagaagatt taaaaaattt ttctaaagta tttgatgaat atgattatag
    17401 aaaacaactg atagtagata ttttaagtag tgagaatcat agtataaatt cttttgacgc
    17461 agtagttggt cgtggtggac ttatgaaggc tataaaaggt ggtacctata ctgtgagtga
    17521 agaaatgatt gaggatatga gaaatgaaat aaatggggaa catgcatcaa atttaggtgc
    17581 attattagct aaaactatag ctgatgaaat aggagtacaa tcatttgtag tagacccagt
    17641 ttctgtagat gaatttgatg atgtatcgag gattacagga atatcagaca tagaaaaagc
    17701 aagttggtta catgcattaa accataaagc tgtttcaaga aaaattgcac aaaaactagg
    17761 gggaaaatac gaggattata actttattgt agctcattta ggttcaggaa tatcgattgt
    17821 agcacataaa caaggcaaaa tgattgatgg aagtggtggt aggtctgatg gtccattttc
    17881 tccagaaagg agtggtggat tacttgcata tcctcttata gaattgtgtt actccggaaa
    17941 atatactcat gatgaaatgg tcgcaaaagt aagttcgatt gggggaatgt acgattattt
    18001 aggaacaaag gacatgatag aaattgaaaa cagaatcaag gagggtgatg aaaaggcaaa
    18061 acttataatg gatgcattta tttatcaagt agcaaaagaa atttctatgt atggagcttc
    18121 actttcaggg aatgtagata gagttatctt gacaggaggg atatctcact cagaaatggt
    18181 agtcaaagga gtagcagaaa aagtttcata tctagctcca atagagatag taccaggaga
    18241 gatggaaatg gaggcacttg ctctaggagc acttagagta cttaatggag aagaaatagc
    18301 aaaaacttat taatatttag gagaaagagt atgttaaaag attttaataa tttattagaa
    18361 gaggctaaaa agcttgaacc tgttattgta agtgttgctg ttgcagaaga caaagaagta
    18421 ttaaaggctg tgaaatcagc aacagattta aacattataa agccaattct tgtaggagac
    18481 aaggataaaa tagaaagtat atcaagagaa attggattaa aaggtcatga aataataggt
    18541 tgtcaaaatc ccgatgaatc agttaaaaaa gctgtagaga tagttaaaaa taaggaagct
    18601 aaagttctta tgaagggatt agtaaataca agtgtctata tgagagctat attaaataaa
    18661 gaaacgggtc ttagaacagg aagattatta agtttgcttg cagtatatga attacctcaa
    18721 tatcataaac ttctttattg tactgatagt ggaataaatg tatcgccaaa cttaaatcaa
    18781 aaaaaagata taatgacaaa tgcacttctt actatgaaaa gtataggcat ggaaaatcct
    18841 aaaactgcga tacttactgc aaatgaaatg gtagacccta agatacaatc tacagtggat
    18901 gccaacctac ttgtagagat ggaaaaatcg ggagaaatac caaagtgtat agtggaaggt
    18961 ccaatagcat ttgatgtagc atttgatgca catgcagcag aacataaagg tattaaaagt
    19021 aatattgctg gtgatgttga tttattagta tttccaaata tagaagcagg gaatatatta
    19081 ggaaagtcat ggcttagatt taataaagct aagtgggcag gtatagtttt aggagcaaca
    19141 aatccgataa ttttaggttc acgttctgac acagctgaaa taaaaataaa ttcaatagca
    19201 ttagcgtgcc tagcatcaca ataagaaatt attattaagg aggagaaagt attatgaaat
    19261 atgcaaaaag aatggatatg gtaaaagctt cagctataag agcttctcaa aagaaaatag
    19321 ctcagaaggt ggcaagtggt ggaactgtta tatcttttgc tgcagggctt cctgacccaa
    19381 atttatttcc aatagaagag ataagtgaaa taactcaaaa agtacttaat gaaaggggaa
    19441 aatttgccct agcatatgga cctactaaag gtgaagatga gttgttagat ctattagtaa
    19501 aaagaatgaa agaagaagaa aatattgata caaaagcaga aaatattata ataactactg
    19561 ggtcacaaca agggatagca ttaagtgcta tgattttact agaaaaagga gatatagtag
    19621 ttacagaaaa tccaagttac ttaggtgcaa taaatgcatt tagaccttat gaatgtgatt
    19681 ttttaggagt agatactgat gatgaaggaa tggtaacaga tgatttagat aaattattat
    19741 ctgaaaattc taacataaag gtaatctatg taataccaac atttcaaaat cctacaggaa
    19801 aaacatggtc tttacaaaga agaaaagatt ttatggaagt cgtgaataag tatgatgtca
    19861 tagtaataga agataatcct tatggagaaa taagatttac accagagcct ctacctacac
    19921 ttaaatctct tgatactaaa gataaagtat tatacttagg ttcattttca aaagtattat
    19981 gtccagggtt tagagttgct tggatgtgtg gtaatgtaga catcatagat aaggcagaac
    20041 ttttaaaaca aggtatagat ttacagtcaa atcaattttc gcaagtacaa gtaatagaat
    20101 ttttaaagaa atacaactta aatgaacata tagaaaaaat aagagcagag tataagaaaa
    20161 gatgtctgct aatgttagat gctatgaaga aacatttccc agaggaagct aaatatacag
    20221 aaccagatgg aggaatgttt atatgggttg aacttcctga aagtataaat gtagatgatt
    20281 tattggataa agccattgat gctggagttg cttatgtttc tggagagtcc ttctttgcaa
    20341 ataatggtcc aaaaaatact atgagactta actatacaac aatgtcagaa gaacaaatcg
    20401 ttaaaggtgt tgagatactt gcagaagtta taaaaaaaga actcaattat gcttgtgaat
    20461 aaatataaaa agtgagggtt taaatatggg agaaaaagaa aacggtataa agtttggaaa
    20521 gttaatgttt caacttgtta ttatagcaat atcttgggaa ttagtttata ttataccatt
    20581 tatccaatat acattatatg atccaatcct aaaagcatta gaatgtacaa acactcaact
    20641 aggttttcta cttacaattt atgggttagg taatatattt ggagctccta taggaggctg
    20701 gttagcagat agatttgatt atagaaagat aattttaggt tctgtatttc ttaatggagt
    20761 agtatcattt ttatttgcat ttaatatgaa ctatccattt gcagttgcta cttgggtagg
    20821 ttgtgcaatc acatcattag ttatgaacta tccttctatg gtaaaaatac ttagagttat
    20881 aggaaaagat aatcaaggta aagtttatgg attcaatgaa gctatggttg gagtttctgg
    20941 cgtagtaatg ggagcaatat tcttatatat ttatacatgc ttttcaactc ctgtattggg
    21001 tatgagatgg gtaatgatat ctttaggaat attatcaata gtgatgtgtc cagtattatg
    21061 gtttgtaatt aaagatgtag atatcagaga agacaaagaa gaagaagcaa gtgaaaaaat
    21121 gtctgctggc gattttatga cagtactaaa atcaccaaac acttggctag taggaataag
    21181 catattctgt gtatattcat tcacagttac aatgtcttat tttacaccat atataacatc
    21241 agtacttgga ggttctgtgg cattgtctgg ggcattagca ataataagac aacatggtct
    21301 aaaattgttt ggagcaccat ttggaggata ttgtgcagat aaagtaaaat cacctacaaa
    21361 agtactacta ccaatatatg tatttggtat agcagtaata attttattcc tagtactacc
    21421 tgcatctact ccaatgacta tctttatagc tttaacattt gtagtaggta tattaggtta
    21481 catgggaaaa ggtatatact atgcagttca agatgaagta aaagttccag ttaaatattc
    21541 agcaactact ataggtatag ctgcagcttt aggattttca ccagatgtat tccaatttgc
    21601 attaatagga cactggatag atacttatgg caataaaggt tacacatata catttatatt
    21661 ccagatagca attctaataa ttggtatact atcttgttta tatatattaa aagttaagaa
    21721 gaaaagagaa agtaagttag aagcataaat attgtaaaaa taccaaatta ttaagtttta
    21781 tagcaatact taaaatttaa agtgcaacac accaaaatgt tatcaagttt tctaggtttt
    21841 agtataaagt tacccctaaa ggtctggaat tgagtagcaa ttatcatcta tttatatatt
    21901 tacataaacc tatttaattt aaattaaaag aatttcaaaa ataggtggta attgtactca
    21961 attttacacc tcataataaa tacgaaaatt taaaatctaa ctatatacta ttcaattata
    22021 tattaataat tgtatatggt actatttata tagtaacagt ttcaggcttt tcattacatg
    22081 tattccaatt tatattgttt gattatttgt caaatacata tcgttaaaaa gtttataaat
    22141 ttacgtttat atatcgatta atattctttg ttttagagat agatacttat agcttgtgga
    22201 ttaaaaataa tataaataga agatagattt tttagatata ttgagttgaa gattatatag
    22261 aatgttgaaa catagctatt taaattattg tctataaata taaaatgaat ggtggtgagt
    22321 taagtgaccg atttagttaa caccattaat ggaattgttt ggtcacctgt attagttgca
    22381 atgtgtttgt gtataggatt atatttttca atgaaattaa gattttttca aataaaagat
    22441 gtaaaagaaa tgtttactct tttgcttgaa ggagatagct ctcatgttgg aatatcttcg
    22501 ttccaaggtt ttgctacatc tatggcagga aggataggaa ctggaaatat agctggtgta
    22561 gctgtagcga tagcaatggg agggccagga gcattagtgt ggatgtgcat aatggctaca
    22621 ttagggtcgg caactgcatt tgtagaatct actttagctc agatatataa agatgagcat
    22681 gatggacagt acaggggagg tcctccatat tactttgaaa aaggtcttgg ttggaaaaaa
    22741 tgttcaattt tattttcagt ggttgcaatc ataagttata cattttttat gcctggcaca
    22801 caatcaaata ctctatatct agcaatgaat caagcatttg gattttcaaa aatgacaata
    22861 gcagttacat cttctattgc actaggtata ataatttttg gaggaattaa aagaattggt
    22921 ttatttgctg agaaagtagt acctataatg ggtggagcat accttattat gacattggta
    22981 ataatcatag caaatataaa tctagttcca ggagctatta tgacaatgtt taaatctgca
    23041 tttggatttg gttcggtttt tggagggatg ttaggttcag caatatcttg gggagtaaaa
    23101 agaggtatat actcaaatga agcgggagaa ggttctggaa cttatggtgc agcagcagca
    23161 gaagtgagtc acccagccaa acaaggatta gtccaagctt tttctgttta tgtagacaca
    23221 ctattaatat gtatggctac aggtattatg attataataa caggaatgta taatgttaca
    23281 gatgtttaca caacattacc aggtgtagaa ccaggtccac aatatgtaca gagtgcaata
    23341 aatacattac atcctcaatt aggtgatatt tttatagcaa tagttttact actatttaca
    23401 tttacaacat tattagcctt ctcatacatg atggagacca acgtatctta cttagtgact
    23461 agaattaagg cagaaaaaca tgaaaagcac ataattaatg tctgtagatt tatgcttata
    23521 atatgtgcag caatgtgttg ctttatagaa ccacttacaa gttgggcact tggtgatata
    23581 tgtataggtg tattaacata tataaactta gttggtatat ttacactaaa aaaaccagct
    23641 ataaaagcat attacgatta tcaaaggcag aaaaagatgg gagtaaaaag gagcgataga
    23701 acttttaatc ctatagaatt agatataaga aatgctgagt tttgggaaaa tagatactta
    23761 aaagaggata agcatgagtt aaattaaagt tataataaaa tataaaaatt actaaataaa
    23821 tttagtggga ggtaagattg gtatgaaaga aagaataata caagtggctg attcaaagaa
    23881 agaaaaaatt ctagggctat gtcaatcttt atatgatgaa ccagaaattg ctttgcaaga
    23941 atataaatct gctaaaaaaa tatcagaatt tctaagagaa gagggttttg atgttgagga
    24001 aaacttggca ggtatggcta cagcttttaa agcaaccaag aaaaatggag atggtcctaa
    24061 aatagcattt atagcagaat atgatgctct tccaggcaat ggacatgcat gtggacatca
    24121 tttaattgca tctatgggag ttggagctgg aatagctctt tcaagtatac tggatactta
    24181 caagggagaa gtaagtataa ttggaacacc tgctgaagaa actggagatg gaaagcctta
    24241 cttgatagaa catggtgtat ttgatggcta tgatgcagca atgatgatac atccaaattc
    24301 aaaaacttgt gtaacacctg aaattatagc aataggaggt ctagatttca tatttactgg
    24361 aaaagcatct catgcaggag ctaagccata taacggaata aatgctttag atgcagtagt
    24421 actactatat aataatataa atgcactaag acaacaatta gtagatggaa cgagaataca
    24481 tggtattata ctagaagcag gaactgctgc aaatgtaata cctgatatgg gaaaagtaag
    24541 attagagata agggcaaaag aacaaaatta ctttgatgaa gtagtagaaa aagtgaaaaa
    24601 ttgtgctaga ggtgctgcaa tagccactgg atgtgaactt gaattttatc actttgaacc
    24661 aacttgccaa ggattaaatg aaaataaagt tttggtagac atatttacaa agattatgga
    24721 ggaatttgga atatatgaag atgaacaagt aatgttaggt tcaacagata tgggaaattt
    24781 aagtcaaata atgccatgta tacatccttt gatgaagttt tctgaaaatg gagaagaact
    24841 acatacaaaa gaatttttgg aggcttctat aaattcatat gcaaaagata gagtaattga
    24901 tggtataaaa atattagctt taacaggatt taatttattt gaaaatccag agttattgaa
    24961 aaaaatgaag gaagagtaga acttagacta tttttaatta tactctttaa aataagaaga
    25021 gtgtctcaaa atgaactttt tagtttatga gacactcttt tttgcatgaa attaaatata
    25081 tttttattat tttaacaatg aaaaagaggc ttatctgttt ttatataacc ttttttaact
    25141 tgtagcacaa aatatcatga atgattacaa ttcgaatgat aaaatcgatt ataagatata
    25201 taggaggggt acatatggaa actttagaag taagaaatat tgaattgagt ggtacaaatt
    25261 atgagatagg atacagacta ggagaattgg ttgctaatat gccagagata atagaaggtc
    25321 agattaataa atctaatctg gtaagcaaaa aagaagaaaa agagatgata gagttgtttg
    25381 ataaatattg tccaggtctt aatgaagaat tgcaaggttt tgctgatgca attcaagtga
    25441 attgtaatca aattttatat tatactatga cttatcttaa accaggttgt agtcaagttg
    25501 cattaacacc agaattaact gaaaatgggc atgtactatt tgcacgtaat ttcgatttct
    25561 cacacaatat ggaggatttt gttttatgta aaactaaagt aaatggaaaa tatgcacata
    25621 taggtactac cgttatgcaa tttggaagag gtgaaggtat gaatgaatgt ggattaggtg
    25681 taagtcagtc atcatgtggt attcctgtag gcaattcaga tggtttgaga aaaccagcaa
    25741 ttgtaggttt acagttttgg gctgttatta gatatttgtt agaaaattgc aaagatgtag
    25801 atgaagcatt agaatattta aaagatatgc caattgcata caatattaat ttattgcttg
    25861 cagataagtc tggaaacatt gcacttgtgg agactttaga tgggaaaaaa gaagtaaata
    25921 taatcaacag tttagaaagt aagagagaat attttttaca ctctactaat catacacata
    25981 ttgataaact tcataaatta gaaccacaat caatgaaaaa ttctattcat agatataaat
    26041 taattaaaga atatataaat aaggctaaaa aaattggaga gaaagagtta atgaatttat
    26101 tgtctttaaa gtatccaaat ggcttatctt gtaattacta taatgatttt tttggaactt
    26161 taaaaagtat agttatggat ttaaatatag gaaagtttaa tattttatgg ggtggactag
    26221 agaataaatg ggagagctac tatttaaaaa atgacattaa atctataaca cagaggataa
    26281 acataaatat tgaaaaagca ccatctgact tttttgattt cattgaataa aaaatgtatt
    26341 taattattct aaataattag gctatataaa tcaactaaaa gtttttattt atcgtattga
    26401 attagttata atattttagt atagtaaaaa atagtgctat tttacatatt taaagagttt
    26461 aagtagaaga gaggtgccat attggattac ttaaagtatt ttgaaaaagc tgtaatgtat
    26521 attgagaata atttacatga aaatataact gtaaatgatg tggcaaaaga aactggatat
    26581 tcttattatc atttaacacg cttatttaag tctatgtttg gtgagagtgt aggaagttat
    26641 ataaaaaaaa gacgattagt aagtagtaca aaagaacttt tatatagtga taaaaaagtg
    26701 atagatatag ctattggtag tggatttgaa tcttcggaag catttagcag agcatttaaa
    26761 tctatataca aggttagccc tatagaatat agaaaaaata gaattgatgt atttgttggt
    26821 aaaaagaaaa aattagagtt agattttatg agacatcttg ttggaaatat aacgattaaa
    26881 ccagttataa aagaaataga aaaaataaag gtaataggta taaaggataa agtaatttta
    26941 gaaaataatt cattacctga cttgtgggag aaattcagaa aagtacatca tattgtccca
    27001 aatactttgc catcaaagag agtttttgga atctgtgaag caatctctga aatacaccta
    27061 gttagtgaaa gtatggaatt taatgagata ataggacttg aagttaatag ttatgatgtg
    27121 attccaaatt catttgtttc aaaaactatt aaggaaggaa agtatgctat atttactcat
    27181 actggtagct tagataactt agacaaaact tatgaatata tatggggtac ttggttttta
    27241 agttcaaaag aagaactgga tataagagat gattttgaag tatatgatga acgattttta
    27301 ggtcctagta atgtgaactc acaaattgat atttatatac ctataaaata atctataaga
    27361 aaactaaaag ataatttgat taagaaatag atgttcgtaa aagagcatct attttttatt
    27421 tttatcagat ttagataatt ttaataataa aaga
//
LOCUS       DAEPLF010000029        26648 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEPLF010000029 DAEPLF010000000
VERSION     DAEPLF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26648
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.029"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            1..681
                     /locus_tag="KR314_001140"
     CDS             1..681
                     /locus_tag="KR314_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBH0327534.1"
                     /translation="MLIGMFWIRSKDRYCREPLIHLIKFFLIGAFLSVIIILLENLLM
                     KFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLDGIIYSVFLALGF
                     ATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKYKFEGNKNKRREY
                     LFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLEKYMNHSKKVFFG
                     NLRKKKKK"
     gene            760..2070
                     /locus_tag="KR314_001141"
     CDS             760..2070
                     /locus_tag="KR314_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBH0327535.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            2170..2706
                     /gene="lepB"
                     /locus_tag="KR314_001142"
     CDS             2170..2706
                     /gene="lepB"
                     /locus_tag="KR314_001142"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBH0327536.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            2872..3408
                     /gene="lepB"
                     /locus_tag="KR314_001143"
     CDS             2872..3408
                     /gene="lepB"
                     /locus_tag="KR314_001143"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBH0327537.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            3418..4173
                     /locus_tag="KR314_001144"
     CDS             3418..4173
                     /locus_tag="KR314_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBH0327538.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDIRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVREG"
     gene            4247..5914
                     /locus_tag="KR314_001145"
     CDS             4247..5914
                     /locus_tag="KR314_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBH0327539.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVILIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            6471..7400
                     /gene="glsA"
                     /locus_tag="KR314_001146"
     CDS             6471..7400
                     /gene="glsA"
                     /locus_tag="KR314_001146"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBH0327540.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            7498..8061
                     /locus_tag="KR314_001147"
     CDS             7498..8061
                     /locus_tag="KR314_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0327541.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            8549..9385
                     /locus_tag="KR314_001148"
     CDS             8549..9385
                     /locus_tag="KR314_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBH0327542.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHIGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            9637..10632
                     /locus_tag="KR314_001149"
     CDS             9637..10632
                     /locus_tag="KR314_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBH0327543.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRNKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(10889..11293)
                     /locus_tag="KR314_001150"
     CDS             complement(10889..11293)
                     /locus_tag="KR314_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBH0327544.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     FLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            12293..13207
                     /gene="queG"
                     /locus_tag="KR314_001151"
     CDS             12293..13207
                     /gene="queG"
                     /locus_tag="KR314_001151"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBH0327545.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFVDNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEMPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            13313..15550
                     /locus_tag="KR314_001152"
     CDS             13313..15550
                     /locus_tag="KR314_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0327546.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNRLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDKSDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNESSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            15747..16799
                     /gene="nth"
                     /locus_tag="KR314_001153"
     CDS             15747..16799
                     /gene="nth"
                     /locus_tag="KR314_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBH0327547.1"
                     /translation="MKNVNLKEINEKDVIVEEDINLKGMDKKDVIEKEEVNKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKEIDKMISKKDEIIDEKDIENFRYIEEDKKSIKVSKKST
                     KKGETEKKTKKSSSNKATQKQSKEVKSKSKIKSESANKKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            16993..17463
                     /gene="trmL"
                     /locus_tag="KR314_001154"
     CDS             16993..17463
                     /gene="trmL"
                     /locus_tag="KR314_001154"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBH0327548.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            17499..18347
                     /locus_tag="KR314_001155"
     CDS             17499..18347
                     /locus_tag="KR314_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBH0327549.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVDEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            18652..19656
                     /locus_tag="KR314_001156"
     CDS             18652..19656
                     /locus_tag="KR314_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0327550.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNAALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGIKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            19781..20893
                     /locus_tag="KR314_001157"
     CDS             19781..20893
                     /locus_tag="KR314_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0327551.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSVPTSAEKDKLNSKSKNEEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            21316..22077
                     /locus_tag="KR314_001158"
     CDS             21316..22077
                     /locus_tag="KR314_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327552.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            22080..22898
                     /locus_tag="KR314_001159"
     CDS             22080..22898
                     /locus_tag="KR314_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBH0327553.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            23078..23680
                     /locus_tag="KR314_001160"
     CDS             23078..23680
                     /locus_tag="KR314_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBH0327554.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    24241..24479
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24543..26462
                     /gene="thrS"
                     /locus_tag="KR314_001161"
     CDS             24543..26462
                     /gene="thrS"
                     /locus_tag="KR314_001161"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBH0327555.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
BASE COUNT        10771 a         2493 c         4999 g         8385 t
ORIGIN      
        1 atattgatag ggatgttttg gataaggtct aaagatagat attgtagaga acccctaatc
       61 catctaatta aatttttttt aataggtgct tttctaagtg ttataattat acttttagaa
      121 aatttactga tgaagtttaa tgtatttgaa gggtattcag aactaatata tgtatcattt
      181 gtagttgcag gacttgtgga agaaggtgta aaagctctga tactgattcc tgccctaatt
      241 aaagaaaaac actttactga aaagttagat ggtattattt actctgtatt tttagcttta
      301 ggatttgcaa caattgaaaa tatggtttat attttttctg aaagtagaaa tctagcactg
      361 caagttggta ttaatagggc agttatctct ataccagctc atgtaatgtt tgcaattact
      421 atgggctact atatttctaa gtataaattt gagggaaata aaaataaaag acgtgagtat
      481 ttgtttatgg cagtgttaat tccaatttta ttacatggag tttttgattt catattgatg
      541 atagaatata gatgggctat tatattactt atagtttatg taataatttt gtggaagata
      601 aatcttgata aacttgagaa atatatgaat cactctaaaa aagtgttttt cggtaatctt
      661 aggaaaaaga agaagaaata aaatttaaag gaaagacata aataattaaa gtataataaa
      721 tgacacacaa gtaaagcaga ttgaaggggg attatagatt tgacagaatt accaaaggaa
      781 tttttagaag atattgaaga aatactaaaa gatgaatatg atgattttat aaaaagttat
      841 gaggaaagta agactactgg gcttagaata aatactttaa aaattgacaa gaaaaaatta
      901 tttgatttgg agttgtttga tttgaatcaa ataccatggg cagaagaagg tttttattat
      961 gatgataaaa ttgacagacc tggtagaaat ccactgcatg aggctggagc atactatttg
     1021 caagagcctt ctgcaatgag tgtaatacca aaggcagata ttaaagaggg tgaaaaagtt
     1081 ctcgatatgt gtgctgctcc tggtggaaaa tctacatata tactatctaa gctaaacgat
     1141 acaggtttat tggtttctaa tgaaattaat gctagtagaa ttaaggctct aggggaaaat
     1201 ttagagcgat ttggagcgag aaattgtatt ataacaaaca ccgattctaa tagcttaaga
     1261 aagacgttta atgggtattt tgataaggtt attatagatg caccttgttc tgggcaaggt
     1321 atgtttagga aggatgaggt tgccatatca gattggagtt atgcaaaggt agtagaatgt
     1381 caatccatcc aaaaagagat tatcagagat ggatataaaa tgttaaagaa ggatggcatt
     1441 ttaatttact caacttgtac tttttcaagg gaggaaaatg agcatgttat aaatgagttt
     1501 atcagtgaat atccacatgc agaacttatt gaaatggaaa gattatggcc tcataagata
     1561 aagggagaag gtcattttgt tgccaaaata aaaaaattag aagaagaaga atgtaatgtc
     1621 aaagaattaa aaataaaaaa tttagataaa gaaataaagg attataggga gtttgaaaag
     1681 aaatttttaa aggttgattt ttatagtaat aaaaaaaata aattttattt aagaggagaa
     1741 aatttgtacc tacttcctga aatatatcca gatacgaaaa aactaaaagt attgagatac
     1801 ggtttgcatt taggggtttt aaagaagaat agatttgagc cttctcatgc actatctcat
     1861 tacttaaaag ttgaagatgt aaaaaatgtt gaaaacttca gtgtacagag tgaatcaatt
     1921 cttaaatatt taaaagggga tgttgtgaat tctaatgata gtagaggatg ggttttggtt
     1981 tcggttgaag gaataccttt aggttgggga aaagaatctt ctggtgtaat taagaatcat
     2041 tatcctaaag gtcttagaaa agtattttaa actattagaa aaaagaagta taatatttat
     2101 attgtaggtt tatggtagat tattttgctg tagattgact taatatttaa cacagaggag
     2161 acgattttag tgggtgaagc agttaaaaaa gaagttgtag aatggataaa agtgattgtc
     2221 atagctcttg ttttggcatt tgcaataact cgttttatag tgccaacaat agtcaaagga
     2281 gaatcaatgt atcctacatt agttgaacgt gattatttga tagttaacag aattgcgtac
     2341 aaggtaggag agccaaaata caaagatata atagtattta aaaccgactt aacagaggaa
     2401 aatggaaaga aaaaagattt agtaaaaaga gttatcgggg ttcctggtga ccatgtaaaa
     2461 atacaagact ccaaggtata tgtaaatgat aagttgttag atgagacttc ctatatacat
     2521 aataatcgta ctgatggaga tattgatatc gtagttccag aaggaaaatt atttgcaatg
     2581 ggagataata gagaaaaaag tttagatagt agatacgatg aggttggatt ggtcgacgag
     2641 cataccattt taggaaaggt tctagtcaga ttgtatccat tttctaagat aggaactatt
     2701 gactaaacat ataaaatttg gagcaatcgc tgattagctg aaatcaccag accatacaca
     2761 atagttgtct atataaaaaa gacaacacct tatttagtag acttatctaa aagatagtgt
     2821 ttaagagaca ttatcaataa agataatata actataaagg agaatttgtg aatgaatgaa
     2881 actattaaag aagagattgt agagtggata aaaataatta ttactgcact tttttttgca
     2941 tttattataa ctcgttttat aaaaccaaca ctagtaaatg gagaatcaat gtacccgaca
     3001 cttaaatcac atgattattt ggtagcaaat aggatgacat ataagttatc agaaccaaaa
     3061 tgtggagata taatgatatt taagactgat ttattacaag agaatggaag gaaaaaagag
     3121 cttgtaaaaa gggttatagg tgttcctggt gaccatctaa aaattaagga ttctaaggtt
     3181 tatataaatg gtaagttatt aaatgaagtt tcatatatac atgataatta tactgaaggc
     3241 gatattgata tggtgattcc taagggaaaa gtatttgcga tgggagacaa tagagaagtt
     3301 agtttagaca gtagatataa agaagtggga ttggtagatg aagaaaatat taaaggaaaa
     3361 gttattttaa gagtatttcc ttttacagat ataggtattt ttgagtaggt gatttgtatg
     3421 gcagttggta tagcgtcctt attgtttaat attgaggagg ctttaaacat ttgtgagagt
     3481 ataaagcaaa taactcattt agagattgga attgacaata tatctgaatg tagcgagtta
     3541 tgtaaatata aagagagaat ttctaagtta ggattatcta tagggataca cttgcctatg
     3601 gaattaaata cttgcgaaaa catagagtat atacgaaatt catggataaa ttttattgag
     3661 aagatagaat ttgaactaaa aggatttgat ataaggtatt ttaaccttca tttgggatat
     3721 gttatgacaa atagagttag aaagaataga gacaaatatt taggaaatag tgtggatttt
     3781 ttagataaat taaatacaaa ttcatatgta tgtatagaga atacttattc aaaaggtggg
     3841 gatttttcaa atattggaaa tatatcttat gattttgaat atatatttaa gaggattaaa
     3901 aattctaaga tttgtttttg ttacgatact ggacactgtt taattgatga ggatgcttat
     3961 gtaaagaact taaaggataa aataagactg atacacttaa gtgataatga tggtatcaat
     4021 gatacacatg ttggtatagg aagaggaatt ttatctgaag aggggattaa agaggtctta
     4081 acattagatg cagaatattt agtattagaa ataaattacg aagacattga agatactata
     4141 agtaaattga ataatattgt aagagagggt taaatacctc tctttatctt gttgatatgc
     4201 ctcattaact aaaatttata ttcatcaaaa ggaaggagat gttgaagtga aaaaaataaa
     4261 cttaaaaata gaaggaaaag ataaggaata ttcattagaa gaaaattccc ctggaatcag
     4321 attgggagat atagcgaaag aattttgtga tgagcataag ggttatataa cacttgcagt
     4381 tgtagacaat aaactaaaag agctaaattg tagagttaag aaggattgtg agataaattt
     4441 tttagacact actaatgaag atggagagag agtttacttt agagtcatgt catttatatt
     4501 tgtaatggct tgtagagaaa tcttttggga tagcagagtt acaatagaac attcactatc
     4561 agatgggcta tattgtgaag ttcatataga tagaaagctt aaagaagcag atgttgaaaa
     4621 tataaaaaac aagatgaaag agatagtaaa caacgattat gttatagaaa aaattgaagt
     4681 tacaagaaat gaagctattt ctatatttga aaataatgaa atgtatgaaa aggcagagct
     4741 tttgaaatac aaagagtatg atagtgctaa agtatataag tgtagaaact atatagacca
     4801 tttttatggt tatatgttgc catcaactgg ttatataaaa tcatttgata ttaagcttta
     4861 taatggaggc gttataattt tagggcctag tgaagaagat aagactcttc ctatgaagtt
     4921 tgtacctcaa cctaagctat caagtgtata ttatgaagca gaagaatggt caagacttat
     4981 gggaattgac aaggtagtat ctcttaacaa aataatagaa aacaatgaat atggagatat
     5041 aataaggaca ttcgaagcat tgcatgaaaa gaagttatcc caaatagcgg atatgataaa
     5101 ggatggcaat aagagagtaa ttttaatagc agctccatct tcatctggaa aaacaagttt
     5161 tgctcacaga ttatcaatac atcttagagt aaataattta aatcctattt caatttcttt
     5221 agatgattat tttatagata gaaaacatac tcctttagat gaatatggaa attatgattt
     5281 tgaatcaatt tatgcaatag atttagagaa gtttaactta gacttaaaga aacttttagc
     5341 tggtgaagaa attgatgata taagattcaa ttttaaagaa ggtaagagag aatacactgg
     5401 taaaaaaata aagatagatt caaatcagcc aattatatta gaaggtatac atgggctaaa
     5461 tcctatttta acatcatcta taccagatga agataaattt aagatataca taagtgccct
     5521 tactcaaata aatttggatg accataatcg aatccctact gctgatttga gaatgataag
     5581 aagaattgtt agagactata attttagagg atatagtgct gataatacta ttttacaatg
     5641 ggcatcagtt agaagaggag aaaagaagaa tatattcccg taccaagagg aggcagatgc
     5701 tatatttaac tctgcttgtg tatatgaatt agcagtttta aaacgatttg cgaaaccttt
     5761 gttggaagag ataaaggaag ataatcctgc acatattgaa gctactagat tactaaagtt
     5821 tttgcagtat tttgttgaat tggacgatac ttctgacata ccaggtattt caattcttag
     5881 ggaatttatt ggaggaagta aaatagttga ttaatatgtg tattatagat tgttttatga
     5941 agattaaatg gcatagcaag tagtgtgatt ggtttatgtt aagtatatta agtgtaaagt
     6001 ggagaattaa attgatacag attttaaagt ttgagttgat atataaaaag gcttgtttta
     6061 tacttttgta tgaagcaagc tttttatttt aaattttact tttttgttta atattttgtt
     6121 agtttatgtg ataatttttt aaaagagtaa aagagtaaaa gagtaaaaga gtaaaagagt
     6181 aaaagagtaa aagagtaaaa gagtaaaaga gtaaaagagt aaaagagtaa aagagtaaaa
     6241 gatgttaggt tagaaatttt tgacgttata agaatttaaa ttactggatt attagattac
     6301 taaattacta gattattaaa ttattaagct atttaaactg tttaaattgt ttgaaaaatg
     6361 aattgattct tgtagaaaat agttttaaag tataattttt tttaacactg ttttattata
     6421 gtacaaactt gtgctaaaat agaatatata acataatata agaggtgcat atgaatacgt
     6481 tagatgagac attattaaaa gaaattataa gttctaataa gaagtatact aattatgggc
     6541 aagtcgctag ttacatacca gaattaaaaa acgctagaag aaatgattta ggaatttgta
     6601 tcatagatag cgaaaataat ctttatagtg ctggaaactg tagcacaaag tttactatac
     6661 aaagtatttc aaaaccaatt gtgcttgcta tggcacttat ggataatgat tgggaagacg
     6721 ttttttcaaa cgttggaatg gagccaagtg gggacccatt taattcaata atgaaacttg
     6781 aaattaatga cacaaaaaaa ccctgtaatc caatgataaa tgcaggggct atagtaacta
     6841 cttcccttat aaatgggagt tgtttagaag aaaaagaaga aagaatgctt agtttcttta
     6901 gaaagttagc aaaaaatgat aatataggaa taaactatga tgtctataag tctgaaaaaa
     6961 tgactggaga tagaaacaga gctatggctt acttattgaa aagtgatgga tttatcagag
     7021 ggaatgtaga agatgttcta gacctttatt ttaaacaatg ctcaattgag atagattctg
     7081 tagatttagc gagaattgga ataaatcttg caaattatgg agtagatata gaaaatggtg
     7141 agcatttaat gagtgaaatg gtttctagga ttgtcaagac atttatgatg acttgtggaa
     7201 tgtatgatgc ttctggggaa tttgctatta aagttggcat tcctgcaaaa tctggagttg
     7261 ggggtggtat aatggcttct gtgccgggac gcatggggat tggtgtttat ggacctgcgc
     7321 ttgacaaaaa aggtaacagc gtcgcaggag ttaaagtttt agaggagttg tcaaataaac
     7381 tcaaattaaa tattttttaa aatattaaga aaggattttt ttgacaacaa catataattt
     7441 aatatataat taaattgtaa agaaatatga attaaataaa ttttttagga ggggtcaatg
     7501 aaagaggtat tggtattacg agatttggag tgcataaagg ctattgcgca tccacgtaga
     7561 atagatattc taaaagcatt tgacaaatcg ccattgtcag caaaacaatt atctcaatta
     7621 ttggaagaac ctcatgcaaa aataaactac catataaaaa tgttatacaa agttggtatt
     7681 ttagaattag ttgaggaaaa aataaagtcg gggattgtgg aaaagtacta ttatccaagt
     7741 gcaaaaaatg tggttatagg gaataggata cttaattttt ctttagacaa tggggaagaa
     7801 aaagaagaat tgtacatatc aaaatttgaa aatatgagtg aagtttttta taaggcagca
     7861 gaggaagatg ttttagaaaa tgaaaatata gttgattatc atgatatttc tcttactcat
     7921 gatgaattag ttgaacttag tgacactatg aagtcaaaga tagatgaaat tttaaataaa
     7981 agacaacaca atgttgaagg ttctaagtat gatatagcta tggttatagt tcctactctt
     8041 gaggaggaat gtcctagtta gagaattaat tatggattaa ttaaagatat aatattagag
     8101 attgaacatc aaaatttact taataatcaa aaaatagttt ttggtatggg taaaacaatg
     8161 atttttaatc tctatttttt tgttttctta tacctattgt atttgtaaat aatatgtata
     8221 gtgtaagatg tatacactat ctgagaagtg cttagataat atacaaagta tgtaagatta
     8281 taatatttga aattgattaa aatgggtata attaaagtgt aaatattaaa attaaaaatt
     8341 aatataaatt aaatcaatgc tagaaatatg ttaaaagagg aatgagatta gttgggatag
     8401 aacatccctt agagcttatg aatttttagt gaaaaagtac tatcaaatag gaatctccat
     8461 cttcatatgt tggctaaata tagttaaggt ggagtagttc aaaattaaaa ttgagtacaa
     8521 gtcgtagtac aaataaaagg agattaatat gattaaatta ggaacacatc tttcaatagc
     8581 aaaaggattt gctaatgcgg ctgagacagc tgtatatatt ggtgcaaata catttcaatt
     8641 ttttagtaga aatccaagag gaggaaatgc taaagaattt gatgaaaaag atataattaa
     8701 atttcaagaa attagaaaag aaaataattt tgcaccactg cttgcacatg caccatatac
     8761 tatgaatctg ggtggtacaa aggatgatgt atacgaattt gcaacaaggg tgatagatga
     8821 ggatataaga aggatggact cattaggaat tgaatacatg tgttttcatc ctggaagtca
     8881 tataggtggt ggtgtagatt ttggtataga tagaatagta aaaggattga acaatgccat
     8941 aaagggtgat gaaaatataa ctatacttct tgaaactatg tcgggaaagg gaactgaaat
     9001 aggttttaaa tttgagcaat taaaaagtat aatagacaaa attgagcata aagaaaaaat
     9061 aggtgtgtgt cttgatacat gtcatatttt ttcagccggt tatgatatag tgaatgattt
     9121 agatggggtt ttggaagaat ttgataaggt tataggcata gaaagattga aaactgtcca
     9181 tctaaatgat agtatgatgc catttggaga caaaaaagac agacatgctc ctatagggga
     9241 aggaaagata ggattaaaag cattaattga ttttatggaa catcctagtc taaaacattt
     9301 accatttttc ttagaaactc cttttgatga tgaaggtcac aagagagagc ttaaaatgat
     9361 aaaagaaata ctatctagta aataaacctt tattaaaaat atgtgaaata ctccgccctc
     9421 aacataatta aggtggagat acaatcatga aatgtgataa tttaaattaa tggataggaa
     9481 taacaaaaat tgtatacaaa ttaactgggt ataaagtatg attatattat gaatagtatg
     9541 taaatatatt gcaaagattc tcataatttg ttgtataatt aaaggtaaca tatttatatt
     9601 atataaaaaa ttattaaaag ttaggtgggg aaagatatga gaaatttagg caatactaac
     9661 atgaaaataa agagagttgg ctttgggggc attccaatac aaagaattac tcaagatgat
     9721 actaacttag ttataaatga gttagaaaaa caaggaataa actttattga cagtgcaaga
     9781 gggtatacaa taagtgagga agcaatcgga attgccattg aaggaaaaag aaataaattt
     9841 ttcttagcta ctaaaagtat gtccagagat tatgattcaa tgaagagaga tgttgaaata
     9901 agtttaaata attttaaaac tgattttata gatttatatc aattccataa tgttaaagaa
     9961 gaggagtatg ataatctttt taaagataaa atggcttata gtgccctttt agaggcaaag
    10021 gagcaaggta agataaaaca tattggtata actagtcaca atttaaatac tatagaaaaa
    10081 gctatagaag atggaaaatt tgacactata caattccctt acaatatagt tgaaggacaa
    10141 gcagatgaag tgtttaaaaa agctcatgaa aaaggaattg ggataatagt aatgaaacct
    10201 ttagctggag gtgctttaga caatgcaaca ctggctatta aatatatact atctaaagat
    10261 tacatagatg tagttatacc aggtatggag agtgttgaac aggttagaca aaatgtcgct
    10321 gtacttgaaa atcttgttct agatgaaaaa gataataagg aaattgaaga aataagaaat
    10381 tctttaggta aaaaattctg tagaagatgt gaatattgta tgccttgtgc tgtaggaata
    10441 aatataccat tgagtttttt atgtgaaggt tattatacta gatatggatt aaaagagtgg
    10501 gctaaagaaa aatatgaggt tatggatgtt aaacctactg agtgtattga ctgtgggctt
    10561 tgcgagagta gatgtccata tgaacttcct ataagagaaa tgcttaagac tgttgttgaa
    10621 aagctaggat agattttatt tattgaaatc ggtgtattaa atttactccg atttcttttt
    10681 attagatgtg gtcttatata caaatattaa atttacatag ttatagctat tatataaatt
    10741 taatatttgt ttaatactta ttaaatattt gcttaatgct ttttaaaaac atttgtttga
    10801 gtatagacat aacacaatta ttattgttgt attagtatag tttcaaatac tggagtatca
    10861 atataatgtc gaataacaca taaaatcttt atttttcaaa aatattatag gatatatcgt
    10921 atttagatac tggataaagt tcaatttcaa ccctagggtt atctttatct ataaaatcaa
    10981 ataaaaatac tggcttaagt tgtttatcat tttttattat tccacttttt tcaataccat
    11041 cacaaatact ttttggataa ttatgtaaat cccccattct cttatttgga aagaataatt
    11101 tcaagactgt tattagattt tcttcaacag tttccccttg atactgttgg tttataaaac
    11161 ctgcaatcat gttttcgtat gcaagatact tagaatattt tccctttgaa ggcatccatg
    11221 cttgaccatt tatgttatga agcttaaaat tagatttact aataggtcta cctggaattg
    11281 ttattttaat caaatttttc accatcctta tacagtattt attataatct gtatatgatt
    11341 taaaagcaaa ttgttattta aagtgttttt acagggcttg tttgtaaaat tttccacttt
    11401 agatgtagta atataagata agtattttgc ttacaaattt taaaatttga tatatgatgg
    11461 acatattcat attagactat tataagacct tcataatatt aataagtata ggtttagtaa
    11521 agagagtaaa aagaaatcca tataagccaa gtaaagactt aataaatggc aatgtaaatg
    11581 ggatattaat tataagaaaa aatcaatctt tttgcattaa ctataggaag aatgtcaaaa
    11641 tataactaat gatatttgaa gttttagagt ttatattaaa aggtaagaag gttaagtggg
    11701 taaaaaaggt tgaatagatt aaaaaggcta agtatctaaa agagttaagt aggtaaaaaa
    11761 gttaaataag taaatgaaaa aagctaagta tctaaatgag ttagtagata aaaaaattaa
    11821 atagataaaa agattaagta tctaaaagcg ttagtagata aaaaaattaa atagataaaa
    11881 agattaagta tctaaaagcg ttagtagata aaaaaattaa ataaataaaa aagactaagt
    11941 attcaaaaga gttagtaggt aaaataatta aatagataaa aagactaagt atttaaagga
    12001 gttaagcaga taaaaaagtt aaataagtaa atgaaaaaga cgaagtatct aaaagagtta
    12061 gtaaataaaa aagttaaata agtaaataaa aaagatgaag tatctaaaag agttagtagg
    12121 taaaaaacta aataggtaaa aagacgaagt atctaaaaga gttagtaggt aaaaaagtta
    12181 aataaataaa aaagacgaag tatttaaaag agttagtagc tagaaaaggt taaaaagact
    12241 aaaaaaatta aataaattta aaaagtcaat tagattaaaa aggaataatt ttatggataa
    12301 taaggaatta aaagcatttt gtaatttaat aggattaaac tgtgtaggag tagcaggggt
    12361 agataagtat gataatttag aaaagatact taaggacaga caacaaaaag gttatttaac
    12421 tggtatggaa gaaccaataa ttgagaacag aattaaccca agacatataa tgaaggatgc
    12481 taactctata atagtctgtg catttccata tcatataaat agacaagaaa agaataaaaa
    12541 ttcaaattta tcaaaatatt gtcatggaaa ggattatcac attgtagtaa aggacttttt
    12601 acaacaaatt tgtgattata tttctaaaac agtaacagat tttaaataca agttatttgt
    12661 tgataatgga ccattagttg acaggtattt agcttattta tcaggaatag gatattttgg
    12721 aataaataat aatatcataa ctgatgaata tggttcttac gtatttatag ggtatatagt
    12781 aaataattat aaatttgaag aagataaacc actagaaaaa agttgtttta aatgtggtaa
    12841 gtgtgtaaaa tattgtccgg gaaatgctat attgggaaat tatgatatga accccaaaaa
    12901 atgtttatca tatataactc aaaaaaaagg agatttgtca gagaaagaaa aagttgtttt
    12961 aaagaatggc aaaaaggtat ttggatgcga tatttgtcaa gaagtatgtc cacataatgt
    13021 tgaaatgcca acaactcata ttttggaatt taaagaaaat ataatagatt atttggatta
    13081 tgatgagata caaaatattt caaataaaga atttaaaagg agatatggag atagagcttt
    13141 tagctggaga gggagaaata taataaagag aaatatggaa ataattttag ataagtatga
    13201 tgactaataa taagagtctg tttcatatac tttaatgtgt atttacttta acacttgttg
    13261 tgtaaaagtc atattatatc attaaagtta aatatgggga ggtgggggaa tattgaaata
    13321 taataataca tctgaaataa atccactaga ttatatattg ggtcaagaaa gagctgtaga
    13381 agctatggaa ttagggttga aaataaataa tccagcgtac aacatatata tagctggtga
    13441 acctggaact ggtaaaagta cttatgcctt aaaggtttta aatgaatatg catccaaaaa
    13501 aaatacacat aaagattggt gttatatata taattttgaa aaccctagag agcctattat
    13561 agttgaatta gaaaaggggt ttggaagaga acttaagaaa gatatggaga aattaattga
    13621 aagtctttta gaagatttta aaaatgcgtt tgaaagtgaa aattttgaaa tagaaaaaaa
    13681 tagacttcta gatgaatatg aaatagaaaa agatatgctt ttgaagcaaa ttaagaaata
    13741 tggggaagaa aaaggattta aattaaaaca gagtaaaatg ggaatagttt ttatcccttt
    13801 gaaagaagag gaagatgaaa gtgataagtc tgatgaagag ttttacaaag ctaaaagaga
    13861 actagaaaat atggcgatac aggtcgtata taaaattaga aacttagaag aaatagctga
    13921 aaaagcagtg ttagagcttg aagaagagat agctaaactt gtagtagaac ctcatatcaa
    13981 gagattgtgt gaaaaatatg aaaattatga taagattcaa acttatctgg aaaatataaa
    14041 gaaagatata atagaatata tgtatttgtt ctatttagat gaagaggagt taaaagataa
    14101 gtatgataaa gagcatttta ttaaatacaa aattaaccta tttgtagaca atgaaagctc
    14161 aaagaataaa gaatcagcac ctgttgtagt agagataaac ccaagtccag caaatctatt
    14221 tggaaaggca gagtatgatt atgcaaatgg aaatataaag actgatttca caaaactatt
    14281 atcaggagca tttcataggg ctaatggtgg ttatttagta ctttatgcag accaattact
    14341 aaaatataca atgtcttggg aaattcttaa aaagactttg cagactaaaa aagtaatatt
    14401 ggaaacacaa acagcaatta aaccagaaaa tatgccattg gatgttaaat tagtactgat
    14461 tggaagccac tatatatatg atattttata tagatatgat gaagattttg aaaaatattt
    14521 taaagtattt gtagactttg atagtgaaat ggataaaaat gaagataatg aagaaggaat
    14581 agcaagattt atagcatacc aatgtgataa gaataattta aggcatttta ctaaatcagc
    14641 agttgaagag gttattaaat ttagcactag attatctggt gatttagaaa aactatcaac
    14701 taagtttaat aaaataatgg aagttgtaat agaaggaagt gcttatgcag agtttagaaa
    14761 ttcggagtat acagagcagt gtgatgttaa aaaagcaata agtgaaaaga gaaaaagaat
    14821 caatagagtg gaaacacata tggatgaatc aattgaaaat ggatttactc taatagaaac
    14881 agagggaaga aaagtaggag tgataaatgg tctgtcagtc ttaagtacag gagaatactc
    14941 ttttggtaga gcttcaagga ttacagcgac cactagccca ggaagtaaag gcatagtaaa
    15001 tatagaaaga gaagtaaata tgagtggctc tatacataac aaaggagtgc ttatattagg
    15061 tggctatctt tctgagaatt ttgcacagga tttactattg tcactaaacg catatgtttg
    15121 ttttgaacaa aattatggtg gaatagatgg agatagtgca tcttctgcag aattatatgt
    15181 actattatca tctcttagtg gagtatcaat taaacaaaat attgctgtta caggttctat
    15241 aaatcaaaag ggtgatatac aagtagttgg tggtataagt gaaaagatag aaggatttta
    15301 ttctatatgt aaaactaaag ggttaaatgg tgagcaaggc gtcataattc ctagaaaaaa
    15361 tcaaagaaat ctcgttttat cagatgaagt taatgatgca gtgcgagatg gaaaatttaa
    15421 gatatatact gttgaaagag tagaagaggc tatagagata ttgacagatg ttaaatttga
    15481 agaaataaag aaattggtaa aagaaaaatt aatatctttc agcaagatac aaactgtgtc
    15541 aaaagaataa gtttattata gaatttaatg ggtatttatg tataataaaa agcaagaact
    15601 ttaataggtt tttgcttttt ttgtaaatat atataaatta aattcttatt ttgtatgttt
    15661 tatatattga tgtttatttt ttgttgatat gactaaacta tttataaata gtgttttata
    15721 aattggtatt ttaaaggggg ataattatga aaaatgtaaa tctaaaagag ataaatgaaa
    15781 aagatgtaat tgtggaagaa gatataaatc taaaaggaat ggataagaaa gatgtaattg
    15841 agaaggaaga agtaaataaa aaaagtatag ataagaaaga tataattgag aaagaagaga
    15901 tagataaaaa aaatgtaaag aaggttgagg ttcaaaaaga aatagataaa atgattagta
    15961 aaaaagatga gataattgat gagaaagata tagagaattt taggtatatt gaagaagata
    16021 aaaaaagtat caaagtaagt aaaaaaagta ctaaaaaagg tgaaacggag aaaaaaacaa
    16081 agaaaagtag ttctaataag gctacacaaa aacaaagcaa agaagttaaa agtaagtcta
    16141 aaattaaaag tgaatcagcc aataaaaaag atgtaaataa gattttagat gagttggaaa
    16201 aattgtaccc agatgctaaa tgtgagttaa attatgggac agcctttgaa cttttaatag
    16261 caactatttt atcagcacaa tgtactgatg taagagtgaa taaagttact tcagagttgt
    16321 ttaaaaagta caacacagct agagactttg ctaatttgag cattgaagaa attagtaaag
    16381 agataaagag ttgtggtctt tacaaaagca agtctcaaaa aataaaggat acatctgaac
    16441 agctatgtga attatatgat ggtgaagtac ctgatagctt agaaaagctt ataaaattac
    16501 ctggtgttgg aagaaaaacg gcaggggttg tacttagtaa cgcttttaat catccagcaa
    16561 tagctgtaga tactcatgta tttagagtta gcaatagaat aggaatagtg gacgagccaa
    16621 atcctcaaaa aacagaattt gcacttatgg aagctatacc aaaagaaaga tggtcacact
    16681 cacatcatgt attaattttt catggaagaa gaatgtgtaa agctagaaat ccagaatgtg
    16741 caagttgccc aataaaagaa gattgtaatt attataaaga acttaatgaa acgaaataac
    16801 ttttagaaag taagttgtac aagagggatt ttctgatata ataatacttg tgaaaatgtt
    16861 agataaaaaa ctattatata catgaaataa aaattattat taacattatt ttatataata
    16921 gtataatatt attgttattc tgtttataaa ataaaatttg ataaataaaa gcgatactta
    16981 gggaggatta aaatgtcatt aaatatagta ttagtagaac cagaaatacc tcaaaataca
    17041 ggaaatataa taagaacctg tgcagcaaca ggttcaactt tacatttagt aaaaccactg
    17101 ggtttttcat tggatgataa acacctaaaa agatgtgggc ttgactattg ggatatagct
    17161 gatatacaat attacgatag ctttgaagaa ttacaagaaa agtatccaaa tagtaaatac
    17221 tttttttcta caacaaaggc taaacagtca cattcagatg tgaaatatga agaaaattgt
    17281 tttatagtat ttgggaaaga aacaaaggga ttaccagaac ctttgttgaa agcaaatatg
    17341 gatacttgta ttagagtgcc tatgttggat gttgaaaagg ctaggtcatt aaatttatca
    17401 aattcagttg ctattgtagt gtacgaggct ttaagacaaa taggataccc aaatatgagg
    17461 tagtttcaac aagcctaaat gagaaaggag catacttaat gaaaaatcta aagataatct
    17521 gtgacagttt atcggacgtg ccaaaagatt tgttagataa atatgatata gatatagttt
    17581 cattaacagt tatactggag gaaagagaat acaaggatag aattgatata tctggagatg
    17641 aattttataa aaggcttaga gaagaaaatg tatatccaaa aacttctcaa gctacttatg
    17701 cccaatttaa ggaaattttt gagaaatata caaatgagga tagagagata ctttatatag
    17761 cagggtctgc cacagctaca gggacgtatc aaagtgctgt aatggcaaag aatgatgtgg
    17821 atggtaaaat atatacatat gactctaact tgctttgtat gggaactggt ttacttgtaa
    17881 taaaagcagg agaacttgca agtgaaggaa agtctgttga tgaaatatta cctgttttgg
    17941 atgaattaaa agagaaggct tttgttatat tttcagtaga tacattggaa tatttacaaa
    18001 agggaggtag aatatcatct actaaggcag caataggaag tatattaaat ataaaaccga
    18061 ttcttgaaat aagagatgga ctagtttcgc aaatatctca agtaagagga aagaagaatg
    18121 taatttctaa gatgatggaa ttgataaaat caaattgtgg ggatgattta tcagaccaaa
    18181 ttgtgtatat aggatatagt gatgatttta aggaaaaaga acaattatct aaggttatgc
    18241 aagatgaatt aagacctaaa gaaattagat ttttccaaat tggtgtgtgt ataggtgcgc
    18301 atgctggtcc tggtgtcact ggtatcatat gcttaaaaaa taaataatat atttttggat
    18361 gacattattt atttttttat taaattagga tagaatatta attattgaga tgttaaattg
    18421 gatttatttt ggatgaaatt aagtgttgat atattagatt tagtttgtag ggaagtgtgt
    18481 aaatagataa gaatctattg cacacttttt tatagtaaga tttattaaaa aaatttcaaa
    18541 atgtacttgt aaattgtata taaaaaaatt ataaatagga tgtaaaagta aataggatgt
    18601 aaaagtaaat aggatgtaaa aatatcgtaa atgtatgagg agtgaaattt aatgcgtttc
    18661 aaaagaatat tgatattgat gttaactgta gcaatgatag ctggaatgtt ggttgggtgt
    18721 gctaataacc caagcaataa tcctagctct agcaaagata ataaaagtga tataagcgat
    18781 aaaagtaatt caaaaaaatc tgtatcaatg atactagata ttgaaggaac aaataatgaa
    18841 gctatgaata atgcagcttt attagcatta aacaatgctc aaaagaaact taatatagat
    18901 acaaacaaag tggaatcaga tgattcctca actttttcta atagtataga tattttatgt
    18961 aatgacaatt acgatttaat tatagctgta ggagctagat ttgcaaaacc attggagatg
    19021 gtagccaaga aatatccaaa gcaacaattt gcaattatag actatgagta tgacaagcaa
    19081 ccttctaata taacttcaat ttcttatgaa gataataaat ctggatacct agctggatta
    19141 attgctggaa aaatgactga aagcgataag gttggtttta taggaggaat aaagagttct
    19201 agtagggata aatttgaatc aggttttaga gagggtgtta aattttctaa tagtagtata
    19261 aaggatattt cagttgagta tgcagatgtt tttaaagata gcaaatcagt agaatctata
    19321 gctaaaaaaa tgatggataa tggagtggat attatttttt ctactactga agatgattca
    19381 aaagctgtaa tagatgcagt aagggcaaaa aataaaaaag ttatagcaac gaataaagac
    19441 caacatgagt tagctccaga aaatgttata agttctattg taaaagattt tgagaatcct
    19501 acttataatc tgatacaaag ttttgtaaaa ggaaattata aaggcggaaa agttatagaa
    19561 aacacagtta agtctggaag tactgggctt gcccaaaata gttctcaaaa tattcctcca
    19621 gatgtgttag aatatgttaa taagaacaat aaataatcta ctataatatc caagaatact
    19681 tatgacttta gttatgaggt gaattggatg ttataataga catatataga aagacgtatg
    19741 cagaggtagg cagacctatc taaatcaggg agtgaatttt gtgaaaataa aaaggatatt
    19801 gatggcttgc tttgtagtag tttttacaat ttttatatta gctatatgta actttgattt
    19861 tggatttgat aaagtaaata agaataattc taatatagat agtgctaaat cgaaacagag
    19921 tgagtctaaa agtgtaccaa cttcagcaga aaaggataaa ttaaatagta aaagtaagaa
    19981 tgaagaagat gacacagaag atgattcata tgtaagaaaa actattggag tattgttaga
    20041 taccgaaaac ttaaataacg aatcatataa taataatgta ctatcagcct taaaaagggc
    20101 tgatgaagat tttgatgtta ggataaaact tgaaaaacca aagacttcta aaagttttaa
    20161 agagagtata gaaaaactac ataagtatga accagatttg ataatagcag ttggagctag
    20221 atttgcagag ccaattaaag aagctgctac taaatatccc aaacagcaat ttgcaatagt
    20281 tgactatact tataaagttc agccagctaa tgtaatttct gtatcatttc aagataatgt
    20341 tactggatat ttagcagggc ttattgcagg taaaatgact gatactggga aagtcggttt
    20401 tataggcgga gttgagggtt ctagtagaga taagtatgaa tctggattta gaaaggggct
    20461 taaaacttca aataaagatg ctgagttact agttaagtat tcaaatatat ttgaaaaccc
    20521 tgaatcagct agaactattg caaaggaaat gagagattct gatagagatg ttgtattttc
    20581 agctacagaa gatgatagta aaagagctat tcaggtagca agagaaaatg gtggtaaagt
    20641 tatagctatt aataaagacc aatacgatat ggctccagat aatgtaataa gttcaattat
    20701 aaaagatgta gaacaacctg tctatgaact gataaagaac gttgcaaaag atggttttaa
    20761 gggaagtaaa gtaatggaat ttaaaataaa agatggagaa cttggcttga cttcaaagag
    20821 cagtagaaat ataccaccag atgttttgga atacataaaa aaagagtata acaaagtgaa
    20881 agatacatat taattattga tgtagaagat tataaaaata tactgtttaa gtttagatta
    20941 gcaataaaaa gatatattta ttaaatttgt gctaactgta caaaatatat tgattaagtt
    21001 tagattggtg ataaaaagat atatttatta aatttagtct aactgtacaa aatatattga
    21061 ttgagtttag attagcaata aaaagatata tttattaaat ttgtgctaac tgtacggaat
    21121 atactgctta agtttagatt agtgataaaa agatatattt attaaatttg aaccaactgt
    21181 acaaagctat actaattaag cttagattaa ctatataaaa ttataacaat aagtgtgtgg
    21241 gcagaaaaat tctgctacac actttattta tattcatata aaaaaattat caaaaaaaag
    21301 aaaaaggagt aaaaaatgga atttacaaga gaaccattta tactagatga cgatttgtgt
    21361 ctagctttgg tggacaaaag aattagttat ctaatggaaa atgaattaaa attaagaaat
    21421 atagagataa taaaaacaat agaatgtaaa gaattatatg aaagcataaa atatcacccg
    21481 gatatatgta tttgtaattt aggaaaagga gatataattg ttgcgccgaa tgtatataat
    21541 caatacaaag agttattaag tagatataaa tttaatataa ttaagggaga agctaattta
    21601 ttaaatagat acccttatga tataccttac aatattgcga tagttggtga ctatgctata
    21661 cacaatttta agtatactga caaagcaata ttagattata ttgaaaataa taaacttaaa
    21721 aagataaata tagagcaggg ctattctaaa tgctcaatct gtatagtaga tagaacgtct
    21781 ataataacat cagataaagg tatttggaaa agtatgaaga atacagaaat agagtgttta
    21841 ttgatagaaa aaggtcacat aaatttattt gagatgaatt atggatttat aggtggatgt
    21901 acagggttga tttctaagga taaactagct ttttgtggag atgttaaaaa acatcctgac
    21961 tatgaacgta taaaaagttt tgtagaaagt aaaaacaagg agatagttac acttagctgt
    22021 gaaaatttac tagatatagg ttctatagtt cctctcatga caagaaagga gcgataactt
    22081 tggttgagaa ggataatatt tcttccatga aatttttgag gaataataat gtaaacgata
    22141 gattaatata tacgaatgac attgtagaag tagtgataga ttctgcaaag caagaagtgt
    22201 taaataaaaa aatacttaat ggtactaaag ctgaacaaag agctttaaat attgatgaga
    22261 tatcaacgga aataactagt atgataataa gtataatgaa agagaaactt ttgaaagaat
    22321 acattctaaa atgctatggg cgtacatatc ctgaagataa aaataatata tacatatatt
    22381 cactgaacat atttgctaaa aaagagattt ttatgagaga aacagtattt actagggtga
    22441 gaaattatat agaagaaaat gattttataa atgtagaagg ttttattaga tttagaatga
    22501 gagaatttat gaaatatatt tctgctatag gtgatattgc agttgaggag tatttaataa
    22561 agaaagacca agatgaattt ataagagttt taaaatactt tatagatact caagaagaaa
    22621 agattgattt gcttaaagtt catataatgg aagataatac atttgtttta tacgataaaa
    22681 atggaaataa gatagacagt atagatgatg aagaaattat aaatatggta ataagagaaa
    22741 atttaaatta tgaggacttt ttaataagta ccttattaac tctatgtccc aaaaaaatag
    22801 aaatattgga tttgttaaat aataattgct caaaggaaat tatagatact gtagaagcaa
    22861 tttttgaaaa taaagttagt ataattatgg aaaactagaa tctaagcata taaaagctag
    22921 ccatataaaa ttaaaaaaat tctttttaaa tcgacacaaa agacttataa ttgtatataa
    22981 agctatagta taactagagt tattaagtat aaagaatatc tacttgatta atgcaatata
    23041 gctggcaaat atacaattga taaggagtaa ttttataatg gataatttaa ttaggatact
    23101 gatattagct gttattggtg gatttatagg ttatgttacg aatgtagttg ctataagact
    23161 gatatttaga ccaatagagc ctattaaaat acctatttta aatatagaaa tagtaggtct
    23221 tataccaaag agaagagcag aaatagcagc aaatgtagga gaaataattc aagaagaatt
    23281 tctttctatg gacgaaatac ttgcaaatat cattactgac gaagataagg aagaagttgt
    23341 aagatacata aaagcaaggg taaaaattat aatacatgag aaagtttcat ttatcccgag
    23401 tggtataaaa aacatgatac aagattatct aggagagata attgagtctg aagttaagca
    23461 aagtatagat gaattgagta aaaatataat aaataaagca aatgaacgta tagatataca
    23521 aaaaatggtc gaagataaga ttaatgaact agatttatat gaattggaag agataataat
    23581 aagaatagct aagaaagaat taaaacatat agagtttttg gggcttgtct tagggttttt
    23641 aataggtata gctcaagggt taattactat gtttatttaa atatatgaga agtagcattt
    23701 atttaagtac taattatatt tacctatttg agtgaattac ttattaatag tatttagata
    23761 ttaattgtat ttacctattt gagtgaatta cttattaata gtatttagat attaattgta
    23821 tttacctatt tgagtgaatt acttattaat agcatttaga tattaattgt atttatttat
    23881 ttgattgtgt tacttattaa tagtatttaa gtatttactg tactacttat taactatatt
    23941 aagtattgat tgtatttaag tatacaaaac ctaaaaagag tacaaaaatt agaagttagt
    24001 tttaataaaa attagtttta gtatttatct agtcatatct atcatgcatg tggatacgtt
    24061 ttaaaaattt taaatttatg aaaatattat aatatcacta taaatcaaga gattaattta
    24121 atataaaaat ataagaatat tggtttacag ctgattgaga tgaaaaataa tgggtaatta
    24181 atatattaca ttaatgttga ttgatgtata actatctgtt ataatgaaaa tatattaaaa
    24241 atcatagtgt tgaagaggaa gagtagagta gaaaccattc tcagagaatt tgggtctatg
    24301 gctgagagcc catgtaatag gtctatttga aaactacctt ggagctgagc ttattagcag
    24361 tatttgtatt tgtactatac gtgaaagtgc tcgggtgacg accttatagt catcaagagg
    24421 gttgtatata cttgtatacg ccaacttggg tggaaccgcg aattaatatc gtcccaaaca
    24481 tatttggggc gattttttat tacaaaaaat ctaaaaatta tttcaataag gaggaaaaaa
    24541 caatgattaa agttgcttta aaagatggct caataaaaga atttgaaaat gctatttctg
    24601 ttatggatgt agctaaatcc attagtgaag gattagctag aaatgtggta gcagcgtctg
    24661 tgaacggaga ggttgtaggt ctggaccaca ttatagatac tgattgtgac ttgaatttat
    24721 ttaagtttga agataaagaa ggtaaggaag tttttagaca tacatcagca catatattag
    24781 ctcaagctat aaaaagactt tacccagaag caaagcttgc tataggacca agtatagaaa
    24841 atggattcta ctatgatata gatttagacc atagattagt tccagaggac ctagagaaaa
    24901 tagaagctga aatgaaaaaa atagctaaag aagatttaaa aatagaaaga tttgaacttc
    24961 ctagaaatga agctttagag cttatgaaag aacaaggaga agactataag gtagaactta
    25021 tatctgattt acctgaaagt gaaataatat ctttctacaa gcaaggtgat tttactgatt
    25081 tatgtagagg accacattta ccatctacta aaaaggtaaa agctgttaaa ttacaaagtg
    25141 tagcaggtgc atattggcgt ggtgacgaaa ataataagat gcttcaaaga atatatggaa
    25201 cttcttttga gaaaaacaaa gacttagaag agtacttaca tttactagaa gaagctaaaa
    25261 agagagacca cagaaaatta ggtaaagaat taggattatt tatgatacct gaagaaggtc
    25321 caggattccc tatgttttta ccaaaaggta tggaacttaa aaatgaatta ttaaaattct
    25381 ggagagaaat acacagaaaa gctggatata ttgagataga atctccaatc atattaaata
    25441 gaaaattatg ggagacttca ggacactggt atcattacaa agaaaatatg tatacagtta
    25501 aaatagatga tgaggattat gctataaaac ctatgaactg tcctggtgga ttaatttact
    25561 ataactctca actacattca tatagagatt tcccaatgag agttgcagag cttggtagag
    25621 ttcatagaca tgagttatct ggtgcattac aaggattaat gagagttaga gcatttactc
    25681 aagacgattc acatatattc atgttaccag agcaaattaa ggacgaaata aaaggagtag
    25741 ctaacttaat agatggtatc tacaaaactt ttggatttga atataattta gaattatcta
    25801 caagaccaga gaactctatg ggaagcgacg aagagtggga agcagcagaa aatggattaa
    25861 gagaagcttt agaagagtta ggtctaccat atactattaa tgagggtgat ggagcattct
    25921 atggtcctaa gatagacttc catctaaaag attgtttagg aagaacttgg caatgtggta
    25981 ctatccaatt agatatgcaa cttccaagac aatttgataa tacttatatt ggtcaagatg
    26041 gtgaaaagca tagaccagtt atgattcata gggttgcttt tggtagtata gaaagattta
    26101 taggtatatt aatagagcat tatgctggta aattcccagt ttggttatct ccaactcaag
    26161 ttaagatact tcctatatct gataaattta tggattatgc aaatgaagtt aagaaagagt
    26221 tatttgataa gggtataaga gttgaattag atgatagagc tgaaaaaatt ggatttaaga
    26281 taagagaagc tcagcttgaa aaagtgccat acatgttaat agtaggtgaa aaagaagtag
    26341 ctgataataa tgtttctgtt agatcaagag ataaaggtga aataggtagt attaaattag
    26401 atgaatttat agctagtatt tcaaaagaaa ttgaatcaag agaaagtatt attcaagact
    26461 aatatataat atattagtag ctaagatatt gttatataag ataagtttaa tcaatatgtt
    26521 gatatgttga tacatagtga ttaaatttat gaaaagatga gtataattag aggtacagtt
    26581 aatcatattt ttatgattaa tgtacctcta gttttttgta tggtagattt attaataaat
    26641 atatcata
//
LOCUS       DAEPLF010000030        26555 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEPLF010000030 DAEPLF010000000
VERSION     DAEPLF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26555
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.030"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(182..376)
                     /locus_tag="KR314_001162"
     CDS             complement(182..376)
                     /locus_tag="KR314_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327556.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NIWADRVRANSYSYENKIAK"
     gene            complement(685..2214)
                     /gene="lysS"
                     /locus_tag="KR314_001163"
     CDS             complement(685..2214)
                     /gene="lysS"
                     /locus_tag="KR314_001163"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBH0327557.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2229..2708)
                     /gene="greA"
                     /locus_tag="KR314_001164"
     CDS             complement(2229..2708)
                     /gene="greA"
                     /locus_tag="KR314_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBH0327558.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2817..3785)
                     /gene="dusB"
                     /locus_tag="KR314_001165"
     CDS             complement(2817..3785)
                     /gene="dusB"
                     /locus_tag="KR314_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBH0327559.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3922..4692)
                     /locus_tag="KR314_001166"
     CDS             complement(3922..4692)
                     /locus_tag="KR314_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBH0327560.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4711..5301)
                     /locus_tag="KR314_001167"
     CDS             complement(4711..5301)
                     /locus_tag="KR314_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0327561.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5485..6453)
                     /locus_tag="KR314_001168"
     CDS             complement(5485..6453)
                     /locus_tag="KR314_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBH0327562.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6446..7423)
                     /locus_tag="KR314_001169"
     CDS             complement(6446..7423)
                     /locus_tag="KR314_001169"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBH0327563.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKTHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7737..9707)
                     /gene="ftsH"
                     /locus_tag="KR314_001170"
     CDS             complement(7737..9707)
                     /gene="ftsH"
                     /locus_tag="KR314_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBH0327564.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9741..11129)
                     /gene="tilS"
                     /locus_tag="KR314_001171"
     CDS             complement(9741..11129)
                     /gene="tilS"
                     /locus_tag="KR314_001171"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBH0327565.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(11312..11740)
                     /locus_tag="KR314_001172"
     CDS             complement(11312..11740)
                     /locus_tag="KR314_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBH0327566.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11757..12563)
                     /locus_tag="KR314_001173"
     CDS             complement(11757..12563)
                     /locus_tag="KR314_001173"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBH0327567.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12765..13250)
                     /locus_tag="KR314_001174"
     CDS             complement(12765..13250)
                     /locus_tag="KR314_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBH0327568.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13301..13960)
                     /gene="spoIIR"
                     /locus_tag="KR314_001175"
     CDS             complement(13301..13960)
                     /gene="spoIIR"
                     /locus_tag="KR314_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBH0327569.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14105..14785)
                     /locus_tag="KR314_001176"
     CDS             complement(14105..14785)
                     /locus_tag="KR314_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0327570.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14769..15659)
                     /locus_tag="KR314_001177"
     CDS             complement(14769..15659)
                     /locus_tag="KR314_001177"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBH0327571.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15777..17327)
                     /locus_tag="KR314_001178"
     CDS             complement(15777..17327)
                     /locus_tag="KR314_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBH0327572.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17421..17687)
                     /locus_tag="KR314_001179"
     CDS             complement(17421..17687)
                     /locus_tag="KR314_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBH0327573.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17835..18695)
                     /gene="yabG"
                     /locus_tag="KR314_001180"
     CDS             complement(17835..18695)
                     /gene="yabG"
                     /locus_tag="KR314_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBH0327574.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18779..19717)
                     /locus_tag="KR314_001181"
     CDS             complement(18779..19717)
                     /locus_tag="KR314_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBH0327575.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19827..19988)
                     /locus_tag="KR314_001182"
     CDS             complement(19827..19988)
                     /locus_tag="KR314_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327576.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19998..20495)
                     /locus_tag="KR314_001183"
     CDS             complement(19998..20495)
                     /locus_tag="KR314_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBH0327577.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20610..21320)
                     /locus_tag="KR314_001184"
     CDS             complement(20610..21320)
                     /locus_tag="KR314_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBH0327578.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21496..22026)
                     /locus_tag="KR314_001185"
     CDS             complement(21496..22026)
                     /locus_tag="KR314_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327579.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEIVKNVDCN
                     IVQYSNKDERRVKAKANKMAMRLIHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(22043..22273)
                     /locus_tag="KR314_001186"
     CDS             complement(22043..22273)
                     /locus_tag="KR314_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327580.1"
                     /translation="MSKLVSKVKKDLEIKWLGVDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22288..23682)
                     /locus_tag="KR314_001187"
     CDS             complement(22288..23682)
                     /locus_tag="KR314_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBH0327581.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTI
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23808..25061)
                     /locus_tag="KR314_001188"
     CDS             complement(23808..25061)
                     /locus_tag="KR314_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0327582.1"
                     /translation="MENKSNFKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALSLS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGVLGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(25112..26266)
                     /locus_tag="KR314_001189"
     CDS             complement(25112..26266)
                     /locus_tag="KR314_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0327583.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRKLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIKKLK"
BASE COUNT         8131 a         5132 c         2629 g        10663 t
ORIGIN      
        1 ttatgtttgc ccttttctta aggacaagta atactatatc atcttaaaaa tatttcgtca
       61 atactttttt tgttttttat tttaaaaaag gttataactt ttggtttttt taataaatat
      121 aattaaaact aatagatata acccttgata ttacaaacaa tacatgcata tataatttta
      181 cctattttgc aattttattt tcataagaat atgaattagc tctcactcta tctgcccaaa
      241 tatttatact cttaacttta ttaacattag ttgtaccctc aaaaatatcc attgcatcct
      301 tacaaaaata tatcataaca tcaagaacca aaaatccaac acaaaataaa cataaaaaat
      361 taattacttg aaacatcaga cattccccca ataatttttt atatatagta ttctcataaa
      421 ttattctttt taccttctca taaattattc taaatatttt cataataaat tactttcaat
      481 catattttct aaatataaag tctcaatcaa caaaaactaa tatcaatttc taacgtttgt
      541 tggtaattta taaattaaat atctaaaaga atctttgaag catataaaaa gcattctaca
      601 ctgacatttt aaacaaaatt tagctattta ttgctaattt aatagctaaa ctttatttaa
      661 aacttagtag aatgctaata tttcttagtt ttcttcttct ttatttggat tgttatcaat
      721 aggtttcata gtcgggaata ataatacatc tcttatagat ggagagttag ttaataacat
      781 aattactcta tctataccta ttcctagtcc tcctgtagga ggtagaccta cctctaatgc
      841 ttttaagaaa tcttcatcca tttcaaaagc ttcatcatca cctaattctc tttttctcaa
      901 ttggtctata aatcttcctt tttggtcaat agggtcatta agttcagaaa atgcattagc
      961 taactcccac ttattagcaa atgcctcaaa tctatcagtt cttcttgggt cttcaacatt
     1021 tctcttagat aatggagata cttcaactgg atgatgagtt ataaatgttg gttggattaa
     1081 tttatcttct ccaaattctt caaagaaagc atttattact tctcctcttc tcattccagg
     1141 ttttatctct ataccttttt ctctagcaac ttctagtgcc tcttcatctg tatttatagt
     1201 agaaaaatct acaccagaat attctttaac acaatcttcc atactcattc ttttccaagg
     1261 tggtgtaaag tttatttctg ttccttgata gttaactatc atacttcctg tagcaacttc
     1321 agccatatgt gcaataacat tttctgttat ttccatcata tcagtatagt ctgcatatgc
     1381 ttgatataat tctattgcag tatattcagg attatgtttt aaatccatac cttcatttct
     1441 aaacattctt cccatttcat aaactttatc aaatccacca acaataagtc tttttaaata
     1501 caattcattt gctattctta aatacattgg tatatgtaaa gtattatgat tagttataaa
     1561 tggtctagca tttgccccac ctgctatagt atttaatatt ggagtctcaa cttctaaaaa
     1621 tcctctatca tctaagtatg ctcttaatgc ttttaatgct tttgttctaa ttaaaaaagc
     1681 attttttact tctggattta ctattaagtc aacatatctt tgtctatatc ttagttcttg
     1741 gtcttttaat ccatgatatt tttctggtaa aacttgtaaa gatttacata gtaatactac
     1801 agatttagcc tttacagata tttcaccttt tttagtttta aagatttctc cttcaattcc
     1861 aactatatct ccaatatcat atgttgaaaa tacactatat tcttcctctc ctatagcatc
     1921 tagtcttaca taagattgga ttcttccctc ttggtcttga atatctataa agccagcttt
     1981 cccttgtatt ctcttgctca ttatacgtcc tgctattttt gtagttttac cttcaagaga
     2041 atcaaagtta tcttttatat ccatagaata atgagttcta tcatatctac tttctttaaa
     2101 aggatctcta cctgactctt gtaatttttt tagtttatct cttcttactt gaagtacttc
     2161 actaaggtct tcttgaattt gcgcttcttc attgctttgt tgattatttt tcattttttt
     2221 cctccaaatt atcttcttat ttctaatatt tcaaacttag caactccatc aggaacttgc
     2281 acttccacaa cttctttcgc agctctacct aaaagtgctt tccctacagg agattcgttt
     2341 gatattttac catcatatgg gtctgcttct gtcgaaccaa ctatagtata ttcaacctct
     2401 tcatcaaatt ctaaatcctt aactttaact attgatccta ttgtaacaac attaaggtct
     2461 attttgcttt catctatgat aacagctttt ctaaccatat tttctaattt taaaattctt
     2521 tcttcaactt gtgcttgttc ctttttagct tcatcatact cagcattttc agataaatca
     2581 ccaaaagata tagcaacttt tattctctct gcaacttctt ttctctttac aacctttaag
     2641 ttttctaatt cttcttctaa tttatcatat ccctcttgtg tcaataaaaa ctctttattt
     2701 tcttccatta taaatacaac tccttcacaa atttcttagg aattttccta attatatttt
     2761 aaaaatttta taatcttttt ttaatttatg cgctatatta taagtcacta ttatttttat
     2821 gtcaaggagt cttccatata gtactccaat ttcgacacaa cttcttgata gtcttctatt
     2881 ttgttaattt catcccttaa ttttgcagag tttctaagtc cttttaaata ccaagcgatg
     2941 tgttttctca tttcccttac agctacatac tctccatgtt cttctactgc aagttttaaa
     3001 tgcttaatgg cagtacttat tttttcgtta agagttggct ctggcaaaat ctcgcctgta
     3061 ttcatataat ggtttattct tttaaatatc caagggttac cttgagcacc cctacctatc
     3121 attatagcat cacaaccagt tttgtctaac atatttattg aatcttctat tgtaaataca
     3181 tcaccatttc ctataacagg tatactcaaa tttttcttaa tctctgcaat tatatcccag
     3241 tctgctttgc ctgtataaaa ctgttctcta gttcttccat gtattgctaa agcgcttatt
     3301 ccacaatcct ctgctatctt tgctatttca acagcattta cactattatc atcccagcct
     3361 tttcgaatct ttaaagtcac tggctttttt gagttcttca ctactgccct caatacctct
     3421 tctgcaagct taggattttt cataagtgct gaaccatctc catttttaac tacttttgga
     3481 gctggacacc ccatatttat atcgagaatc tcatttgaat aatcattcat tatctctgct
     3541 gctcttccca taaactctgg ttctgaaccg aatatctgaa ctgctacagg atgttcttcc
     3601 tcttctatct taagcatttt tttagtattt tcatcatcat agcaaagtgc cttaccattt
     3661 atcatttctg tatataaaag cccacatccc tgttctttac aaattaatct aaatggaagg
     3721 tctgttacac ctgccatagg tgatagaaaa actttatttt tcaattctaa atttcctatt
     3781 ttcattgttt ttctcctatt tttgccttta ttttgtgatt taactttaaa ttcttatttc
     3841 ctcattataa tataccacat atagctagaa atctatcata aaaagggaat tggatatata
     3901 acccaattcc ctgtatatta attattcttg gtttttttca tatattattc tcaatccttc
     3961 tagtgttaaa aaaccatcta cataatctat acttttcgtc tctgaagcaa tcagtttagc
     4021 taatccacct gtagctataa ccttaacatc atcacaattc aattctttct tcatcatact
     4081 tattattttg tcaactaaac caacatatcc ataaattatt ccagattgca tggctgatac
     4141 agtactctta caaatagtca tacctggctt agctaattct actctaggta atttagacgc
     4201 actttgaaat aacgcctcac tagatatttt tattcctggt gctattgttc cacccaaata
     4261 ttcacctttt tcagagatag cacaaaatgt agttgctgtt ccaaaatcaa caagtatact
     4321 tggtgctcca tacttttcta tccctgctac agcattaact attctatctg ccccaacttg
     4381 ttttggatta tcatatttta tatttagacc tgtttttatg cctggaccta ctattaatgg
     4441 ctgtttttta cagtacttta tacaaaagtt ttcaagagaa tgcatgacat ttggaactac
     4501 agatgatatt ataacatcat caatatcact tatattcaca ttatcataat caaataggtt
     4561 acttatcagg attccatatt catcagacgt tttttcccta tctgttttaa ttctccagta
     4621 attaactaat ttgtcacctt tatatatacc taaaaccata ttagtatttc caacatcaaa
     4681 tactagaagc atatgtcatt aaccttcttt ctattttttg cttttcttaa gtacagtggc
     4741 aacagcagct actactaaag cccctactat tgcttctggc accccatttg ctgcggcaag
     4801 agccattatt acttttcctg ctgaaccact tttacccatt gcatgaagat acttgtcagc
     4861 atataagaca tatatcattc ctaaaactcc agctgtattt aacagagaac ctattgcccc
     4921 tgtaattaaa cctgatatgt ataccttatt tgtagcctta attattaact gatatacata
     4981 ataagctaat acacctatca taactcttgg taatattgaa actaatggat ttataaaaac
     5041 aaaagatgtt actgttgggt ttgttatagc atttaagaga cttgatatcc caaatataaa
     5101 tcctattgct gccccaacta ttggtccttc cataatagct ccaataataa ctggtatgtg
     5161 cattatagtt gcttttgtag gtccaactgg aataaatccc aatggagtca tagatagcat
     5221 aatagaaata gctgatagta ctcctattat agtcatcttt cttacattta cttttcttac
     5281 tgatgcactt gtgttcatca taaaaaatac acctccgttc cagctcatta agatgccgga
     5341 ctacttaatt ttttaatttt tttcgttttt ttcttataac tatgagtcaa gctaccattg
     5401 taggatgccc gctctaacaa gaataatttt atcaagatga gttgccattg tcaacaattt
     5461 gacaagaaaa atatgctaat ttcatcatat ttctcgtttt tttactatat ctttatatgc
     5521 cattatattt tctacactat ccgctttttt tactgcattt attatacttt tttccacaac
     5581 ctctacagcc aatgaaccta ctaatgttat atctgcttct atttctcctg ttgatattgt
     5641 gaatattgta tctccatcat gaggtgtatg gataggaaaa atagacttag catatccatc
     5701 atgtcccatt tgagatatct ttttacatcc agctttggtt aacttagcat ttgttgcaat
     5761 tatcccaatt gtagtattat ctacactaaa tccaccttta ctttcacctt gtttcattaa
     5821 tttatatgta tcaattgttt ctgtcttttt atcatttaaa gcaccagcta taaccttacc
     5881 attttcataa acatctccaa gagcatttac agcaattaat gcagatacaa ttagaccatt
     5941 atctaatttt atagaatagc tacctattcc gcctttcata gcatattcct gacctcttat
     6001 ttttccaaca gtggctccac aacctgctcc ataatttcct tgatttaaaa tatcagtatt
     6061 tgcaacttca caagctctat aacccatctt taaatctggt ctacatttaa agctccctac
     6121 tcctaaatca aataaaactg ctcctacaac tataggcact ttacctacac ctacatcaaa
     6181 gccaattcct ttttcttcta agtatctact tacaccacaa gtggattcaa gaccatatgc
     6241 agaacctcca gccaaaacta ctgcatgaac tttttgaacc atatttacag ggtctaataa
     6301 atcggtttct ctagtccctg gagcagaacc ccttacatca acaccgcaag tagctccttc
     6361 ttcacatatt actactgtgc acccagtaag accatttcta tcctcaacct gcccaacctt
     6421 taagccttct atatccaaaa tattattata catatccttt ttctcctctt atagaaactt
     6481 cacctgaaat aatttctttt atacttccat cacttctttg aactaataaa tttccctctt
     6541 catttatgtc taaacatttc actagttctc tattatctcc ttttaataca tatatatttt
     6601 tatctataat tgcagaataa tctctacata tttttaaggt tttttctttg gaattattta
     6661 cagtataatc taaatataat ttttcaaatt cagtcaatac attcctaaca atatctactc
     6721 tggataacat atacccttct ttatacaatg atgtggcttt gtcagctatt tcttgttcaa
     6781 aatccattat tttaacattt attccaattc caagaacaac ataatttgtt ctttcaattt
     6841 cagcagataa ctctgttaat ataccacata ttttttttcc atttaaaata acatcatttg
     6901 gccattttat taatgtattt gctcctagat tgtttaaagc tttcgttaca cttgctcctg
     6961 ctattaaagt tataaatgga gctttgaatg gttccatatc tggttttaat ataatactca
     7021 tccagattcc ttcatgagtt tttgaatccc atcttcttcc caatcgtcct ttccccatat
     7081 cttgatgttc acttaaaatc acagtcccat ctacactatt tatagctatc cttttagcat
     7141 gttcatttgt tgaatctata gtgtcaagat gttcaatatt ttttccaata aactctgtat
     7201 ctaatttata gtaaatattc tgtgggctta ataaatcagt tggattttcc gctaatcgat
     7261 accctttttt atttacagat tctatattat atccctcttc ttttaaggct ttcatatgtt
     7321 tccatatagc tgcacgtgac acccctaatt gttttgataa ttcttctcct gaaataaatt
     7381 cagatttatt atttaatata atctctatta ttttatctcg catatttcac ctctaatatg
     7441 acataatttc atgacttatt ttcttctaat aaataattat acattattaa tatactttta
     7501 atactattaa ttttaaattt cctttttaat tattataatg gttgttttat attttatatt
     7561 gatgatttat ataattttac attttattta aataatttta aaataataaa aaactgaaag
     7621 tatcaattaa attaatacat gcaaatataa tttgcttatt aatttaatat tatactttca
     7681 gttttagtat ttaatccaca tcagataata atcttaaaat gaccaaggtc ttaaaactat
     7741 ttatctttat cagatttatc ttctaaattt ttatttatat caataacatt gtttctttct
     7801 tcttcatctt tttctaattt tatagttaat tgttcttcaa cttctttaga agaattttcc
     7861 tctgtaactg cattttcaat ttcattcaga ggtaactctt tattaaatgc ttttacaaat
     7921 tgttctgcat ctaaagtttc atatataagt aaagcttgtg ctacatactc taatctatcc
     7981 atattttctt gtaatatact tcttgtctta tcatatgcac catcaactat acgctttgtt
     8041 tcttggtcta tctcaaaagc aacttcttca gagtaatttc tcttacttga aaaactatta
     8101 cctaggaata cttcatcatc actgtcaaat gacattggac caagtttaga actcattcca
     8161 tactttgtta ccatacttct tgcagtagct gtaactcttt ctaagtcatt tgaagcacct
     8221 gtagatacat ccttaagtac tagctcttct gctactctac caccaagtaa aactacaata
     8281 ttttctttca tttcattttt tgtagcatag aacttatctt caacaggaag ttgcattgta
     8341 aaccctccag ctcttcctct tggaactatt gtaacttgat gaacaggact tacttcttca
     8401 agtacatggg cacaaactgc atgccctcct tcatggtatg ctgtaagtct tctttctttc
     8461 tcacttatta ctttcgattt tttagccaca cctgctataa cctttgttat agcttcttct
     8521 atagtttcca tcttaatttt cttttctctt tttctagctg ttaatattgc agcctcattc
     8581 attaagttct ctatatcagc aggagtaaat cctggtgttc ttctagccaa gacatccatt
     8641 tttacatcat cacttaatgg tttatttctt gagtgaactt taaatatagc ctctctacct
     8701 tttacatctg gtgtaccaac aacaacttgt ctgtcaaatc ttcctggtct aagtaaagct
     8761 gggtcaagta tatctggtct atttgtagct gccatgatta ttataccttg atttacccca
     8821 aatccatcca tttcaactag aagttgatta agagtttgct ctctttcatc atgacctcca
     8881 ccaagacctg cacctctttt tctaccaact gcatcaattt catctatgaa tatgatagct
     8941 ggagcacttt tcttagcctg ttcaaataaa tctcttactc ttgaagcacc aaccccaaca
     9001 aacatctcaa cgaaatcaga accacttata ctgaaaaagg gaactcctgc ttctcctgca
     9061 actgctcttg atagatatgt tttaccagtt ccaggaggtc ctaccataag cattcctttt
     9121 ggtattcttg cacccaattc aatatatttc ttaggattct ttaagaaatc tactacctcc
     9181 tgcaaatctt ctttttcctc atctagtcca gctacatctt taaatgtaac tctagttttt
     9241 tcatcatctt tatgaacctt agcttttgat ttgccaaaac tcataacctt tccgcctcca
     9301 ccttgagact gattcataaa cacgaaccaa ataatcacca tgaagaatat taacagtaat
     9361 gtaggcaaca tttcgacaaa ccaaggtgta gatggcttag cttcaccacc aaaagttagt
     9421 ttcccagcct tagcttggtc taacacctca tctgcaagtt tatttcccat cacttctgtt
     9481 ggaacatatg acttaaactt tgtatttgta tcttttatag taccttctac agaagtttga
     9541 tttacaaagt ataatcttga tatattctca tctgttaatt ctctatatac ttcagagaat
     9601 tttaaatctt ttattttttc tgtaggctta cctgagaatt gaactatacc tactataatt
     9661 ataaaaacaa gtagataaaa acctgcccct tttagcaact tattcaatat agaagccctc
     9721 ctttcataca ttgctttgcc ctataatttg ttaaaattaa tttttaatac ttcttttgtg
     9781 ttttcatcaa ttttataatt ttcactgatt ttataatctc caacacatat tattccttca
     9841 ctatctgtga ttataggaat tttacatctt tcttctcttg ggatttttaa atctatgaat
     9901 aaatctttca cttttttact tcccatagca agctttatct tatcaccttg ccttctgctt
     9961 cttattacta tacccccttt taccttatta aaatcaaacc ctttagatga attgtctaat
    10021 ttcatactct tatacctatc tatgctcatt acttgggtct caataatttg ttttaattct
    10081 tttattttaa taaacccatt actaggtaca ttgtaataaa attcaatttc ctcattaaca
    10141 atttcctcat tcgttaatat tatactgtct ttttttctat aaacaaatat atttcttgga
    10201 agagttaaca ttttatttac ttttgaatta tcttctaaag acattatatc ttctatatgt
    10261 ctttggtcaa caaaattagt atctcctagt atatgtttta tggaatttct aagaactcta
    10321 actttgatag catcatgtaa gcaaacaaaa tcgtctaaat ttatctctac aaagcctttt
    10381 tcttttatat ttgaaacttc tctaaattta gcttctgcct ctttctcaat ataatcgtta
    10441 tcacttttta aactattgct catcctcact atagattcta ttacatttga attaaaatta
    10501 tccttcatat atggtaaaag gtctaacctt atcttatttc tagtgtatat attttctaaa
    10561 tttgttttat ctatcctcgg atttaaatta taagcttcac aatactcctc tatctcattt
    10621 ctttccacgt ctaatatggg tctaattatg caattatctc taatataatc aattcccttt
    10681 aagcctttta agcctgttcc tctcatgata cgcatcataa cagtttcggc ttggtcattg
    10741 agattatgac ctattgctat tttattggct ttaatcttat ccttaatttc ataaaacatt
    10801 tcatatctta gcttccttgc tccttcttct aatgataatc cctcattttc acaatatttt
    10861 ggtacattaa tagactttac aaagaatata attcccatat cctcacaaag tttagataca
    10921 tataatgcat ctttttgagc ttctatccct cttatttgat ggtttaaatg tgctgcatat
    10981 atttctatat taaaatcttt ttttaatcta tttagtacat gcaataaaca aactgaatca
    11041 ggtcctccag aaagacctaa aactatttta tcgccttttt gtattaaatt atgtttattt
    11101 atagtactta gtaccttatc aaatatcata tttatccctc ttacacttag ttttattcac
    11161 ttttaagtat actttctata aaaaaacgct aaacccttgt atcaatttta agaaattaaa
    11221 gtttaatata aatataataa taccaaataa attaaataaa aggtagtttt ttcactacct
    11281 ttataaaatt tctggaaaat agccatagtt attatttagt acctatgtat atatctattt
    11341 tattcctacc taaaaataaa tcttgtacct ttatattata gtctattttc aattttatgt
    11401 gttccccttc ttgtatattt atatctaatt ctcttgtatc aatatcaatt aataaagtac
    11461 cttgtggggt cacatacttt gtagtactac tttctccaca tttaaacagc attgttgagt
    11521 taatagcacc aaactttttg atactaactt gatttttaag tatctttata gtattagtta
    11581 tttctatatt ttcttctttt tccttgtaaa taacatatat tccatcattt ttataaaaaa
    11641 ttttaccaaa ggcagtcatc tctatggtgt ccatatttcc tttttcatca tattgtctag
    11701 tatttatact tatttttaca ctcaacgatt tttcctccat agattcttca tataaactag
    11761 tatttttgta tttctacttt ttgaatctta tcaatttttt tctttaagaa ctctcttgct
    11821 atatctgaaa atttttcagg tatatctgat acataatatt gataagtccc atgaatttct
    11881 gtattgttta taatattttg cacctctaaa atctttttta aatcctttgc agtctcttta
    11941 gctggattaa caagttttat atgctctcca acttcttcac ctattgttct ctttaaaata
    12001 ggataatgag tacatcctaa cactaaagaa tctatatttt tttctttcaa ttcttgtaaa
    12061 tatatctttg ctgtcaattt agccacttct gtatttgccc atccttcttc aacaataggt
    12121 acaaataaag gacatgcttt attaacaatt tctatactct catctatttt agatatttcc
    12181 aagttgtatg cttttgaact tatagttcct tcagttccta ttattcctac aattttgttt
    12241 tttgtagaac ttacagctgt tctagctcct gcttctataa ctccaattat cggtatatca
    12301 tatttttctt gagcttcttt taagcttctt gcagtagcag tgttacatgc tataactata
    12361 gcttttacac cttttgtttt taaaaagttt atagcttgaa aagtatattt aattatagtt
    12421 tctttggatc ttgaaccata tggtattctg gcagtatctc caaagtatat tatatcctca
    12481 tttggtaata tcttcattat ctcttttaga actgttaatc cacctaatcc cgaatcaaaa
    12541 actcctattg gtttattgtt catctttata caacacctac tttacatttt tgtgtttaac
    12601 aacaacttat ttatcgctaa aatttatatt tatattcata attaatatat tattacctaa
    12661 atgattatta tagtttattt tacacaaaaa ataaaggagc tatcttaaat tagaaaataa
    12721 tttctacaaa aagacaactc ctttcactac ttaaaaattt aaatttattt tgcaaacaca
    12781 tgatttccta ttctcatata aggattcaat ctatttatcc atttgcaagt ggctatttct
    12841 ggattccaaa aatatactgc tttattagtt gggtcagttc cataaagagc ttcctgagct
    12901 gctttacgtg ctgagtctgt tggtgccatc tttattgaac catcaagcac aactgagaaa
    12961 gcattttttt gatatatgac ttcttctatt gtgtctggaa atctagggtc caatactcta
    13021 tttattacta cagctgctac agcaacctgt ccctcatagc tttctcctct agcttctcct
    13081 gcaaccaact tagataataa catcaagtca ttatcagata aatttattac ttctttcttt
    13141 gctatatttt tttcttgttt gtttactttc ttaatatttt ctgttggttc atatgcaaat
    13201 acctctttaa taggcataaa tataaaagtc aaagctaata gtgtagccat cagtttactc
    13261 cacctgtcca tgattaaaac cactccttac attcttattt ttaaaatttt ttctctaaat
    13321 ccttgactac ttcaagtact ttaaacttaa tttttactct acttgttttt tttggtgtat
    13381 cttgttttat taacttatat tctttatcag ttaaaacttc cttcaactta tcatctgtag
    13441 atttatctat cactccatta gattcatcaa caaaacatag tggagggaac ataacacacc
    13501 accagttttt tccttcacct ttcccaatga taatctttaa tgctttatat tcaccagctg
    13561 gaagaactat attagaatat tgtttagtag ggaaattgct atattgtata cctaccttta
    13621 cactgtaatt atatccattt ttaagaataa tatttttact tatttcttcc agactactat
    13681 attccttttt tataatagct tcactttctt ttatactttt tgaattttga agctttggct
    13741 gtaaatattt tattacttca tctctaactt ttagtttaag ttcttgatct tcatctgtat
    13801 tgctgtttgc tataacatga aatcttatta atttatcttt ataatctttt gatatattgt
    13861 ctactttttt tacctcacca tttattacta ttgtcattat tgatatgact gatataagac
    13921 ttaatattaa tagtcccaat ctaatcttaa tttttctcat tgaaaattcc ccctcaagat
    13981 aaatttatat catcaattat gtccaattaa atttaattta ttcataatat tttttaatac
    14041 taataaaaaa agctatctta aaatgattac aatcatttta aaatagcttt ttaaacctta
    14101 tattttataa gtctttacta atcatagcct tatctatcat aaactgctct gctttttcta
    14161 tatgctctgt agcatacttc ttggatagct cactatcacc tttaattata gcatcaagta
    14221 agtatttatg ctcttcaaca atattatgtg atgtatcata agcagacata taggtaactc
    14281 taaatctata tacttgttcc cataatgagt ttattagttg gattaatttt ttattattag
    14341 ttgctttaaa tatacattca tgaaattcta catcaagttt taataacgca tctacatcgg
    14401 attccaaagt gcttttttta aattcttctg atatatcttt aagcttttta atatcttcat
    14461 cactaattct ttcagcggca agatatgctg ctaagccttc taggcttgct cttacttcta
    14521 atacatccat aatatccttt aatgacatat ttgccacata cgcacctttt ctaggcaaca
    14581 taacgactaa accttctaat tcaagctttc ttattgcctc tctcactgga gtcctactta
    14641 ctcctaattg ttctgctagt tgcacttcca ttagcctttg accaggcttt aattttcctt
    14701 ctaagattgc ttctcttaaa ttctcaaaga ctacatctct taatggcttg taattatcca
    14761 agttcagttt agttaaattc tccacaaatt tcaacccctt tttgactgct attaacaaca
    14821 tacacctgtt tatatttttt taataattct ttttttccaa ttagagcatc ttctttattt
    14881 ttaaatagtc caaaaactgt aggaccactt ccactcatca tagaacctaa agcattattt
    14941 ttcatcatta cttgttttat atcacttatt tctttgtgtt tacctatagt aacattttca
    15001 agaatattta ccatgctttc tgatacagct tttatatctt cagattttaa acactctatc
    15061 aaatatttat tatttggtct ttttttaata ttattcaaat ctaatccttg ataaacttct
    15121 ttagtagaaa cgaataagtc tggtttgcat atcaatatat ttgtatcaca tggtaaacct
    15181 ttgatgtttg ttagcttttc tccaatacct tgagctaagg caggtcttcc tgatatacaa
    15241 aaaggaacat ctgcgcccaa attaagtcct atttctttta attcatcttc agataatcta
    15301 agttcccata ggtgatttaa acctactaat actgcggctg cattagaact tcctccagcc
    15361 atccctgctg ctacaggaat atttttttct atgaatattt ctacaccttt ttttatataa
    15421 aatttatttt taagtatttt ggcagcttta taaacaatat tatcttcatc taaaggaata
    15481 tctaaactag tgctttttac ctttatttca tcttcgtcta attcttttat ctttactata
    15541 tcgtataaat ctatcgtttg cattatcatt tcaacaaaat gataaccgtc ttgccttttt
    15601 cccaagacat caatggataa atttattttt gctctactct ttagtcttat aaaatccatt
    15661 ctatcctcct tgtttaaaga ataatgcatc actaacaata atattatact acaaaaaata
    15721 aaataatagg gtattataat accctattat acttaattac tttaaatttt ttaatattaa
    15781 tctactaata cgactttttt ttctaaaata agacacttac caggcttaat tggttcatca
    15841 agttttatat cattaagttc tgcaatttca ttttctgttg tattgtattt ttttgctata
    15901 ttccagaaag tatctccttc tttgcatata tatacgatta tgctaggagc tttagataaa
    15961 tcatatacac cttggtcttc tcctttgata ataaaatttt ctgctttctt atctaatgct
    16021 tcagtaaatc tcttaatttt gataatcaag tctatctggt ccctattcaa atcaacttcg
    16081 actttatcaa tacatgctgt attaaacaca gatgctgtat cagttagatt atccatttct
    16141 atatcatgtt caaatggaat ttcctcacta attttataaa ccaattttaa accttcaact
    16201 gggacaaata aaatttcaac ttttatgatt ccttgtataa tgcttttatt tccttcaata
    16261 taactatttt ctatagacat ggttggacaa acgcttacaa tatcttttat ttgtatatca
    16321 tcattatcat ttcttatgcc ttctctaacc ataaatgttt cttcactacg tcttaaagtc
    16381 ttatttaatt gaataggttt gtggtcaaat tttattatct tttgaggcga atatgcatct
    16441 tgtaatactt ctcttgtaac ttcatctgta acttttacct tacaacaagc catacaatct
    16501 atttctaaaa gacctgtgtt gctttcacta ttttgcttga aaatatgatt aaaatctgac
    16561 atagacaaca atacttcttc agtcatacca tcactaactc cagggacttc tacaaattga
    16621 gtaaactcaa tacctactct atccaactct acaagttcac cctcatatgt acaagctaac
    16681 ggatttattt ctaatactcc accaataata actttattgt cagtcactct actttctttt
    16741 actttaacac atggatttaa acttattata gattgaattt cctctgtgtt tattgttata
    16801 gtatctctta tagaactttc tgctttttct attccaacta tatcttgaaa acaaatttcc
    16861 tttctgtgct tttgtatgcc ttccacttgc gccacatctt ttacaatgtc taacctttgc
    16921 ttttcaaaca aacttcccct tatattcata agagctccta ctttaatctt tctctcattc
    16981 attatagtac aatccatatg ctctacttct gaaaataaca tgtattccat atcttgaaca
    17041 acattgtctt tttctattac ttcgtttata tctacttttc cgtctacatt agaaatagta
    17101 tttttatcat ctgcaatata gattacatta taattaaaac tacctctgca aagtatcttc
    17161 ccatctgcca tttcaatttt tttaagtgat atgtatcctt ctgtttttac aatttcatat
    17221 acatctaatt ttttgtctgg tacaactgcc tccgcttcaa tgaatgtttg aaattttcca
    17281 aagtctattc tattgtcaac tttaattaca tctttaatta attccatagt ctacctcctt
    17341 aagtattata aaaatatcac actatattta tatttaagga gcaaaaaaaa aattacacct
    17401 atttttaaat aggtataatt ttaacttatt tgtaatttat cttgatctct aaaaacttgc
    17461 aactttacat tagatgttaa taaatctgag taactataag atactctagg atatccatta
    17521 ctttcatcat ctaactttat aacaaacacg cttggataaa ctttttctag tataccctct
    17581 tttgtaataa tctgctttct tcctttatta gctttcaaca atattttctt tcctatatgt
    17641 ctctctaagc ttactcttat cttatctaga gtttgaacag tagccacaat atcaccttct
    17701 ttaacgttta tatgtttaca taatatcaca tataaacatt tttgtcaaat gtttaaacta
    17761 attattttat atattttttg caaaaatgtc aatactattt ttgaagatat acagtataat
    17821 tatgttaatt tttgctaatg taatattgtt tttggcattg cccatctaaa ttttcctctt
    17881 gtttcagaac caccaattcc cttaacacct gtaatagtct gctctataac ctcatcaata
    17941 ggaactacta catcaattct tgaacaagca attttctcca ctataaaaac agggtctaat
    18001 gcatgtatca ttattcttcc aggcgaactt gcataatttg ctcctgcact tattattttt
    18061 tcataatttg attggcatgc tcctgcgaat atgacaagat tatccaaatt tggttgccat
    18121 ttacgtgctt cttttactgt tttaacaaaa tttgaagagt ttctatagtt actcatatct
    18181 tctatatctc ctctttttat tgtcattgca tcatgacctg ttattactag tatatccgga
    18241 ttatattttt ctagtagtgc tcttacttct ttatactgat tttgctctga aacagctaca
    18301 cctactgcag gtattccaag ttgagcatat acatccaagc aaatttttaa atattcttta
    18361 tctccatcta tctgtagtac ttttccaggc attccatatg tgttaggatt taattgaagt
    18421 tttggaactg ctctagtcat tcttttttgt ctttctttag cttttcttac tgacttatat
    18481 aataaatttt ctacattttt atctattaat atatctttta tatcaggtgc ttttactaat
    18541 tctaggtcat caatataagc atctgctatt actctaaatg caattccctt tagtatagct
    18601 attttctcat tattttcatc tacaccaaaa gagactattt taaatactat atctttgtta
    18661 tgtgactttc tagctacaat gtctcctacc ttcatagtta aacctccaat aaatttttat
    18721 cttagtttat actatgttcg atataaaact ttgtttccta atgtcaaaaa aacatttctt
    18781 ataatacttc ttttatcact cttaaagcat ttttatagta tatcttttct atatcatctt
    18841 cactaaaccc ttcttttttt agaggttcat acagtttacc catctgagat atatcttcaa
    18901 tctcaacttt tgaatctatt ccatcaaaat ctgaccctag ggatacaaca tctattcctc
    18961 caacatttac aatatgtttt atgtgtctaa ccatatcttc aagtttactt tcacttttat
    19021 cacttaaaaa taagtggaag aagtttatac ctgtaactcc acctttattt gctaatactt
    19081 taatcatatc atcagttaaa tttctagaat gattcatcat tgctcttgaa ttagaatgtg
    19141 tagcaattat tggtttagaa gaaatctttg caatttcata aaatccacca tctgaaatat
    19201 gagaaacatc aaccaacatt cccaattcat tcatcttatg taccacttct cttccaaaat
    19261 cagtaagccc tttttctcta tattctactt tattatgagg aaagctcaat tcattcacat
    19321 gattccaact tattgtcatc attctaaccc ctaagtcgta aaatttcttt aaattttcta
    19381 atttaccttc aagtactgca ccttcttcaa tagataaaag agctgtaagt ttaccttctg
    19441 attggttttt cataatttct tcatagttag tagctaaagc tatttcatca ctattttttt
    19501 tcatttcctc atgaaattta tttgccatac tcatgcaata atcaaaagga tgttttactt
    19561 cttcagtatc aacaaaaaga gcaaatgttt gagctaatga atcccccttt ttcaatctgt
    19621 ctatatcaac agaatattta ttagacttaa gttcacttgt ttctacattt tccattaatt
    19681 ttgctatagt atcacaatgt aaatcaataa atttcatatt tataccccct gtatatttta
    19741 tatttatatc taaaaataga aatagttgtc cttatagaca actattgaca actatttttt
    19801 aatattcttt ttaatattac ttaatcttat tcttctttta agttaggtat tactttttta
    19861 acttcttcaa tttgatgcca tgttttatct atattatttt cactaatata tttttttaaa
    19921 tcagttaaag atgtaaaatc tttaactgat aaattgtcat catcatctgc aaatcttaaa
    19981 ataaacatga gactccctta ctttccacaa catttcttat atttttttcc gcttccacat
    20041 gggcatgaat catttcttcc tatttttgct tctttaacta ttgttttaga cttcttataa
    20101 gcctttatta tttcttttct ttggtctgct ggtagtatat cttcccaacc ttgtaaatta
    20161 tatagccaat gagcctcaac agttaccata ttgttatata atttttccca atctattttt
    20221 atagaaactt ctttatcagc ttctacttct tctaaaacta tatcttcttt taagctttca
    20281 cttattccat ctatgaatcc catgaaaaat tcattagtag ttccaaattt ttcagccaat
    20341 tcacttactt taccttcaac aacatccatt ttattattta atatttcatt gtatattcca
    20401 gcttcaactt tcaaatattc ttcccagaat tttacttctg cttcttgact ttcatgatta
    20461 tcacttaaat ttctccattc agtatataaa ctcatacttt ttcctcctaa gtatacctat
    20521 atttttatag tttttatagt ataaaatctt ttagttagat tggaagtttc ttctttttga
    20581 ttataccata ttctatttaa agttaactct tatattatat cataattaaa caattctttt
    20641 aatactttta tagcatactc ttggtttata aaataagggc ttgtcgcaat tgttattact
    20701 ttggcttttt taattaagtt ttttatcttg taaaatttga gttcaaatgg tatatagtcc
    20761 atatcacttg aaaatatatc caaatctata tcaagaacaa actctgattc tacttctaaa
    20821 tcaaatccat acagactatc tattattatt aattctgaaa atatgttata ttttaaagca
    20881 ggttttataa aacttcccac atttaaaact tcatttgtat acctaaatac atcttttaga
    20941 ttatttacat ctacatcata gttctcaggc tctctagtat ccttatgttg gtctacatgc
    21001 actaacttgc atccttttgt aaattcaccc tttaacaaag attttgccca aaaataaaat
    21061 gcatggttat gattgtcaaa cacatatatc tttttatttc ccaggacata ttcgaccata
    21121 ttctccaatc cttttgcttt tatttcctta ttttcatcta cttcattaaa taccacctca
    21181 ttgcctaacc ttacatcatt taatgtacca tttataagtt ttggaacgta tatgctctta
    21241 ttttctcttt tatcatatga aaatacatta tttcctactg gtttttctat ataaaatcca
    21301 ctatattcat aattttccat aaaactttat ttcctccatt gttatataaa tatataagtt
    21361 ataattttaa aaattaaaat gcacttattg taaatcatat tatctattat agaaaacatc
    21421 ttatataact aaatataata tcttctctca atatgtatta tacaataagt gcaaaatata
    21481 tttatataaa atatactaca ttcctataac aaaaccatca gcttcaattg gaaggtaact
    21541 aactcttcta ttcaactcca cctttccaaa aaatactacc caatatggtc taaaaatact
    21601 ttcaatttta actatttcaa tctctgggat tcctcccata aacctatgaa ttaatctcat
    21661 agccatttta tttgcctttg cttttactct tcgttcgtcc ttattactat actgcacaat
    21721 attacaatcc acatttttta caatttcata tgtaggaaaa tcttgtacta tactaccaga
    21781 acctgtagaa ccattcacta aaatttttaa ttcatttata tcattagaat atctcctatt
    21841 cttgaaaatg gatttcgttt tgtgaatttt aagtgtgata attttaaact ctatataatg
    21901 aagtctaact tcatgagcat atttgttctt taatattagc ttactaaata aagaattttt
    21961 cccactttta gctatctcta tagcttcttc ttttgtaatt cttataggta acgctcttat
    22021 atccataaaa tcattctctt tcttattttt ttcttgtcat ttttaaaact ctatatatat
    22081 aatcaaaaaa tattacaatt acaactgcca taactccata tggaattatt tttccaacta
    22141 taatatcatt tctagtcaat ataccataaa atataaatcc tgttattaaa aagtacaata
    22201 atactagaaa accgtctact ccaagccatt ttatttctaa atcttttttt accttagaca
    22261 ctaatttaga catagcagtt tctcctttta tagattattt tgtgtattta aattttcacc
    22321 tattctatta ccaatcatcc atgcaataaa tgaaaaaggt atagctatca caagaggctc
    22381 taactcaaag ctctgaactg gaaatatttt aaaataacca aaagtcactc cacctacaat
    22441 cattcctaaa ataccaccca tagatgttct tcttccattt ccaagaagcc cacctactat
    22501 aggtacaaat gccgaaccca tccaaatact tcctacaaat atacatgcat catatacatt
    22561 ttcaaatcta aatgctacgc taagccccat aactgctgct aaacatacac ctattctagt
    22621 aaactttact aattgtttct gagataactc tttttttgta attcttccat atatatcatt
    22681 tgctaatgta gctccagctg ctagataata gctatccaat gttgatatta tagaacctaa
    22741 aattcctatt ataaatatta ctcttattcc ttgtggtaat gtatctaaaa ctaaagttat
    22801 atatccagcg gctggagcca tctctggata agtagctctt actataagtc ctgttatagt
    22861 taacaccatg tcaaaacata tccatagtga aaaacaagtt aataaggctc ttcttccaac
    22921 cttaggagaa tctgctgctg aaaatctttg ataaaaagtt gggtctgaat aagctccaaa
    22981 agaatataca attaacatca atgctttcat agctgacata ccttttgtag ggtgcataag
    23041 ctgtggtgca tctttgagtg cttctgtgag accttgaaat cctcctattt gttcaaattg
    23101 acctggcata accatagtta aaccaaatac catacaaaaa aacaatacca tatctgtaat
    23161 ggcaactccc ataagcccac caagtactgt aagtacaacg actactccta ccattattaa
    23221 tcctattaat tctttatcta ttttccatgc tacgttacct atagttccca tagcagttat
    23281 ttctccaagt tgtgcacaat atataaacat caaaattgcc ccaattatag caacatgctt
    23341 accataaaaa ctttccagta aatcaggaac tgttatattg gttcttatat gaatccttgg
    23401 accaacccac aatgctaaag gcattctccc aatatgtgca gctattgacc aaactgccca
    23461 tgcagatatt ccatatatag ccgcatactc tacactacct actgtaccaa ctcctccata
    23521 ccatgatgca accaaagttc ctactattaa ggaccatggt aaacttcgat ttcctaggta
    23581 ataactttcc atatctttta catttctaga aaaataataa cctattccta ataatataaa
    23641 tgcaaatata actataatcc cattttccca aaatgttaac atatctaatc tccacattct
    23701 tatattttaa tttttaactt taatatctaa atatgaacaa ctctacccat ttccataaaa
    23761 ataggtagag ttatataata attataagta tttaagtaat cttgttatta agcaatttta
    23821 ttgtttaaac ctttattttc tcttttattt attttattta aagcataagt agcaaatatc
    23881 cctataactg cacatactac agataaaata aatatatatg tatatcctgt ttgtccatat
    23941 ttatctaacc aattacctgc aatagtgtaa taaaacgcat ctggagaaaa tcctataaat
    24001 gaagcaaatc caactactgc tcctgtagtt tctaaagata tattagactc tgaaacagat
    24061 gcaaagtata tacctctaaa tccaaataaa attacactta gtattatcat gtttattaca
    24121 gctatccata taaatcctgt actcttagga ataaccaagt aagcagctgt acttactgtc
    24181 attaatataa atcctacaaa cattactctt atagatgaac caactttatc tgttataaca
    24241 ccagcaactg gagatgctcc catttttata acataagttc ttattattga taaagcagaa
    24301 actagagtca ttggcattac ataaacttct gataagtatg gacttaaata agttaaactt
    24361 gagaatacta aataagttga aaatattata agaccaatta accaagcctt tggcattgta
    24421 actactttta acaatgactt aatacttaat ccttcatctg atgctccact atcttttttc
    24481 tcttccacta agaaaaatag aagtatacca catattatag atgctgcaga atataaccat
    24541 actacatatt tgaatcctac tgttatttca gcaaatttac caaagaagta aagacctata
    24601 aaagaaatta acgtacctga tacacctgat aacccttcat aaaaaccaaa tattcttcct
    24661 tgttctgact cacttccttg cattctaaca accttaacag atgctgagaa gaaagttaat
    24721 attgttgtaa atccccataa tacaaatatt aagagtatca tactatatga tgggaatgta
    24781 gagaaataaa aaccaagtac tcctgaagca attaaagaaa aagttatcag ctttctaacc
    24841 gagaatctat ctgcaaacca tcctcctata aaataagata ccattcctac taatccatat
    24901 aaactcaata agttccctat ttgtgtatgg ctcaatgata aagcttgttg cattgggtca
    24961 taaaatgatg atttcatata tggtaaacta tacatagccg ttgttccaaa tgccaatatg
    25021 aatattatca agaatctttt aaaattactt ttgttctcca ttaattttct ctccttaaaa
    25081 ttttattgtt tataatctca ttcatgtaat tttattttaa tttttttatt gcttgtttaa
    25141 agtcatttat tatatcattt gaagattcta atcctacagc tattcttatt agattttctc
    25201 cattcatttg aactaatgta tgctcttccc ctaagcttgt tgctattgtt gcaagctcta
    25261 aagagttcat taattttcta ctagcttggt ctcttgtcaa tccattgata ccatccttaa
    25321 gtttaaatga aactacacct ccacctaaac cattcatttg agttttacaa agctcatatt
    25381 gaggatgaga ttttaatgct ggatacttta ccaattccac attctcttct tcctctaaaa
    25441 actctgctac tgctaatcca ttttcacaat gctttttcat cctcatatct aacgttttca
    25501 tacctcttaa aatcatccat gcactcattg gtggtaaaga tgctcctgta aaacaacata
    25561 aacttggcca tctaactaaa tctattaatt cttttgaacc tgctactgct ccccctaaag
    25621 tatccccatg tccatttata aacttagtaa gactatgtat tactaaatct gcacctaaat
    25681 ctattggtct ttgtacaatt ggtgtcccaa aagtactatc aactatagct aagcttttat
    25741 tttcatgagc aatttctgct attgttttta tatctactaa gtctaatgat ggatttaaag
    25801 gactttctat ataaactatt ttagtatttg gtctcattgc ttcttttaca ttttgtggat
    25861 ttactgcatc tacaaatgta acttcaactc caaatttaga taacaactca gacatgaata
    25921 atttagtgtg tgaaaatatt ccatctgaac taactatatg gtcaccactt tttaccaatg
    25981 ctaatatagt agatgttatt gcccccatcc ctgatgtact agatatggca ctttctgcat
    26041 tttcaagcat agccaactta gcttctaact ctgtaagagt aggattacac tctcttgtat
    26101 acacatatcc ccatgtcata cacaactctt caaaatgctc tactgtcttt gcagcaaatg
    26161 ttgctgtttg acatattggt gtagccaatg cacctgttgt tgggtctgca ctatgtcccc
    26221 aatgtattat ttttgtctct aaatcttgat taatattgtt actcattttg atttcctccc
    26281 attttgactt atttatacaa cgttttccct gccaaattta atcaatttag cttagtctta
    26341 atacattttt tcaagtatac ttttttattt gttataattt ctaaaaatat gacacttatt
    26401 ttgttttttt acagttctaa ttataaactc tatagtaact atagagtcaa tatgtttttg
    26461 atattttttt tacaatgttt aaaatagtca gtacaaaaat aggtatatct tagaataact
    26521 aaagtcatcc taagatatac ctatttttat atcac
//
LOCUS       DAEPLF010000031        25840 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEPLF010000031 DAEPLF010000000
VERSION     DAEPLF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25840
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.031"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..561
                     /locus_tag="KR314_001190"
     CDS             <1..561
                     /locus_tag="KR314_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBH0327584.1"
                     /translation="IKKYDINCVVDIRETPYSKYNIQYNKEAFNESLRNSGFIYIYMG
                     KEFGAKRNNKDVYTQEGYADFEKVAKEDIFLNGIERLKKGCQMGYRIVLLGAMQEPIR
                     CHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLDKYFSDREQLSIDNLLGSAL
                     TREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(718..1170)
                     /locus_tag="KR314_001191"
     CDS             complement(718..1170)
                     /locus_tag="KR314_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBH0327585.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            1618..2976
                     /locus_tag="KR314_001192"
     CDS             1618..2976
                     /locus_tag="KR314_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0327586.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTIMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            3249..3629
                     /locus_tag="KR314_001193"
     CDS             3249..3629
                     /locus_tag="KR314_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0327587.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            3754..3954
                     /locus_tag="KR314_001194"
     CDS             3754..3954
                     /locus_tag="KR314_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327588.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            4150..4878
                     /locus_tag="KR314_001195"
     CDS             4150..4878
                     /locus_tag="KR314_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0327589.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            4900..5817
                     /locus_tag="KR314_001196"
     CDS             4900..5817
                     /locus_tag="KR314_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBH0327590.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            5959..7143
                     /locus_tag="KR314_001197"
     CDS             5959..7143
                     /locus_tag="KR314_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0327591.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            7250..7438
                     /locus_tag="KR314_001198"
     CDS             7250..7438
                     /locus_tag="KR314_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBH0327592.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            7588..8238
                     /locus_tag="KR314_001199"
     CDS             7588..8238
                     /locus_tag="KR314_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBH0327593.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            8341..9279
                     /locus_tag="KR314_001200"
     CDS             8341..9279
                     /locus_tag="KR314_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH0327594.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            9305..10156
                     /locus_tag="KR314_001201"
     CDS             9305..10156
                     /locus_tag="KR314_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0327595.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDTIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
     gene            complement(10441..11175)
                     /locus_tag="KR314_001202"
     CDS             complement(10441..11175)
                     /locus_tag="KR314_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HBH0327596.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            11301..12017
                     /locus_tag="KR314_001203"
     CDS             11301..12017
                     /locus_tag="KR314_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0327597.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            12151..12843
                     /locus_tag="KR314_001204"
     CDS             12151..12843
                     /locus_tag="KR314_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0327598.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            12833..14014
                     /locus_tag="KR314_001205"
     CDS             12833..14014
                     /locus_tag="KR314_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBH0327599.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKLLYFNSEI
                     SVINQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKMEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            14296..14751
                     /locus_tag="KR314_001206"
     CDS             14296..14751
                     /locus_tag="KR314_001206"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBH0327600.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            14741..15466
                     /locus_tag="KR314_001207"
     CDS             14741..15466
                     /locus_tag="KR314_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBH0327601.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLNQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            15438..17819
                     /locus_tag="KR314_001208"
     CDS             15438..17819
                     /locus_tag="KR314_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBH0327602.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDNAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSCGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(18024..19253)
                     /locus_tag="KR314_001209"
     CDS             complement(18024..19253)
                     /locus_tag="KR314_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBH0327603.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            19853..20767
                     /locus_tag="KR314_001210"
     CDS             19853..20767
                     /locus_tag="KR314_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327604.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGVKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            20791..21426
                     /locus_tag="KR314_001211"
     CDS             20791..21426
                     /locus_tag="KR314_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBH0327605.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTAQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            21802..22518
                     /gene="pyrF"
                     /locus_tag="KR314_001212"
     CDS             21802..22518
                     /gene="pyrF"
                     /locus_tag="KR314_001212"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBH0327606.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGADFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            22544..23587
                     /gene="carA"
                     /locus_tag="KR314_001213"
     CDS             22544..23587
                     /gene="carA"
                     /locus_tag="KR314_001213"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBH0327607.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGEILTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIAPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            23640..>25840
                     /gene="carB"
                     /locus_tag="KR314_001214"
     CDS             23640..>25840
                     /gene="carB"
                     /locus_tag="KR314_001214"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBH0327608.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYL"
BASE COUNT        10444 a         2699 c         4619 g         8078 t
ORIGIN      
        1 ataaaaaaat atgacataaa ttgtgtagtt gacattagag aaactccata ttctaaatat
       61 aatattcaat acaataaaga ggcatttaac gaatctctta gaaattccgg atttatatat
      121 atatacatgg gaaaagaatt tggagctaaa agaaataata aagatgtata tacgcaagaa
      181 ggttatgcag attttgagaa agtagcaaaa gaagatattt ttttaaacgg aatagaaaga
      241 ttaaaaaagg gttgtcaaat gggttatagg atagttttat taggagctat gcaagaacct
      301 ataagatgtc atagaagtat tttggtggga aaagtattaa ataaagaagg tttcgatgta
      361 aaatatataa tgcatgaagg aaatttggca tatcaggaag atatagagga atcattattg
      421 gataagtatt ttagtgatag agagcaactg agtattgaca atttattagg gagtgcatta
      481 acgagagaag aaatgataca agaaggttat aagctagcta ataaagaaat aggatataga
      541 actgagaaac taggtaaata aaaatataca agtagataaa atacagtact tatcttaagt
      601 aaaataatat tatgaattta taatagctag gcatgatgtg ccaattaaaa atattctagt
      661 tatttaataa tatcattaaa tttagggtat tttttaatag cacatcatgt tgcagtttta
      721 aaaaataaaa tctctaatat tgccataatc aactgttcta ttaagagtag gatgactaga
      781 tataaatcta actgtaattt ttgaatttaa caaacaagga tttgaaatta aaaatactct
      841 atctccattg acaatagaag taatagctac tggcaaaata tctacaaatg tatcatatct
      901 gtttttagca atttgataag gataatttcc tacaaaaaca aatagattta aatcatatat
      961 attatcagta aaccaagccc attgaccata aactttgtcg atattcattg tgcctaaacc
     1021 aagtgtatca aagtatgttg ctatagttaa ataaatattt tccatgtttt ctaaatatga
     1081 aacagtatat cttctgaaac gtatgggtgc tgtggaagaa ataggataaa ccatatttac
     1141 atttagatta tttgcattta aattaaccaa agtatcacat cctaaaatta gtctataaaa
     1201 tacaatattt aaatggatgc gattttgtta catctaaagt gtaatatttt atatattgaa
     1261 aaagtggtaa aagtttggat gattaaataa tattataaaa aatatataaa atgtacttat
     1321 tgttattgct acacgatagt gatacaatat taacgaaata tagctacttt taataataac
     1381 ggaaaatatt tatattaaat taataaatat tttaaatgtg gtatccataa aaaataataa
     1441 acataagata attataaaga tatactgtgt tcaaatgata ttaaacaaga tttatattat
     1501 ttgaaagcag tatattataa tgcgaacaat acaattctaa actttgcatt gtcaatttac
     1561 agttcgataa actaattaaa aattaaattt atataaagag ggaaaagagg aatagggatg
     1621 tttataagga aaaatgttat aaagttagca attcctataa tgatagagca gacgtttgtc
     1681 atgcttttag gagtatttaa tacaataatg gcaggtcata ttggggaaga agctgtatct
     1741 gcagttggaa tggttgattc aataaacaat atatttatat ctgtatttgc tgcattatct
     1801 gttggagcta ctgtcgttgt agctcaacaa ataggaaaaa aagatagtga aaaggtgaat
     1861 gaaactatta agcaggctct agtgtctgga gtaatattat cctttacaat aacaatattg
     1921 atgtgggttt ttagaactgg tgtaattaat ttattttatg gttcagcaga agaattggta
     1981 aaatcaaatg caaaattata catagagttt acattattta cgtatccatt tattgcgaca
     2041 caacaaatag caaatggtat tttaagaggg tgtggagatg taaagaaacc tatgtatgtg
     2101 actatgttta tgaatatagt aaatgtaaca ttaggatata tacttatata tggtattgat
     2161 ttaaatcctc taggaataaa tttgcaaaca ccatcctatg gcataacagg tgcagctata
     2221 tctattgcta tagctagaat aattggatgt atagtaatta taagagcttt atttaaagga
     2281 aatgaattta tatctatggg taaaatattt ccatttaaga ttgatgttga aactcaaaag
     2341 tctatattta atatagggat accagctgga gttgaacaac tactttttaa tgcaggtaaa
     2401 ttgattgttc aagtatttat agttactatg ggaacatcag caattgcatc caatgcaata
     2461 ggaatgtcaa ttgcatccat aataaatgta attgggaatg ctttagcttt gtctgctaca
     2521 actttggtgg gacagtatat aggacgtgga gatattaaag gagctaagag tactttatta
     2581 tatttaacta aatttgcaac aatttgttta tttatagtag gagctatatt tataccaatt
     2641 gcaccctgga tttcaagtat gtatactcaa aatgctagtg taattgacat ctcaacacaa
     2701 ttaattcgaa gtgattgttt agctatggta atatggccta tatcctttgt attatcagca
     2761 gggctaaagg gagccggtga tactagatat acaatgatga ctgcagtaat aggtatgtgg
     2821 atttttagaa tttttacagg ttacgtattg ggggttgtat tagaatttgg agtgttagga
     2881 atttggatag gtatgtatac tgattggctt gttagaggga ctttatattg tttaagatta
     2941 agaggaacaa agtggctaaa acacaaagtt gcatagaata tatgaaatca cttttagatg
     3001 gataactcta tttagaagtg attttttatg tagttattat cataattata agtatgatat
     3061 ataaggttat taaattacat aaataaaggc tataatagta tggatattat aaattatata
     3121 ttgagcagaa ttacataatt tggcgataaa tattagaaat atatatctat agaaaataat
     3181 atatatgggt atattattaa atataaaaaa tattaggtat ttatttaata ataaaggtgg
     3241 gtattgttat gataaataaa ataggaaaaa taactctcta tgtaaataat caagaggatg
     3301 caaagttgtt ttggacagaa aaattgaact ttgtagtaac ttatgaaaag gaaatgggtc
     3361 caaatatgaa atgggtggaa gtagcaccaa gtaaaaatga atttacaaca tttgtgttgt
     3421 atgataaaaa tctaatgatg aagcaaaaca aagagataaa tgtatctcat ccatcaataa
     3481 ttttaagtac aagagatatt aatggaactt atgaacaaat ggcaaataac gaggttgaag
     3541 ttggggaatt aatggagatg ccatatggaa ggatgttttc ttttaaagac caagatggaa
     3601 atgattattt agtcagagag gataaataat tttacaaagt attaaattta ctcaatatct
     3661 gatataatta gagacaataa ttattataaa ctagtctagg attagaatta tataattaaa
     3721 aattaagtgt aaattttgag tagaggtgat aaaatgttta agaaaatggc agtactaaaa
     3781 gatatagcaa ctaaaatagg tcgtaaaaaa gcgtatgaac tattagaaat ggttgaaggt
     3841 aatgatgcct ttgtagctga ggtaaagata aaaaagaatg gaatagaatc taaaaaagaa
     3901 gaaattatgt taaaagataa tcaaaaaata atattagagt atatagaagg ttaaatttta
     3961 aatacatata agttgatata ctaaatttct gagatgagtt tacataaaac tcatcttttt
     4021 tacttttcaa taactttgtt ttattattta atatacattt tatctgctta gagttgctga
     4081 tatataaaaa ttactgatat aataaaagaa tggatattaa tgtaatattt tatagaaaag
     4141 aggggtatta tgcttgaaat aaaaaatctt tcctttaatg ttgagtctaa caatgaggaa
     4201 ctaggaataa taaatgatgt tagcctttca tttgagaggg gaaaacttat tgttatcaca
     4261 ggtccaaatg gtggaggaaa atctacaatt gctaagctta ttatgggaat tgaaaaagct
     4321 acgagtggtc agataatttt agatggagaa gatataacaa atcttagtat tacagaaaga
     4381 gcaaaaaaag gaataggata cgcatttcaa caaccaccaa gaataaaagg tatgacagtt
     4441 gaaaatttac ttacacttgc tcatggaaaa ccattgtcta ctgatgtatg ttgccaatac
     4501 cttacagatg ttggattgtg ttcaaaagac tatttaaatc gtgaagttga taactctctt
     4561 tctggagggg aaatgaaaag aattgagata gcaactcttt ttgctagaga tttaaaggtt
     4621 tctatatttg atgaacctga agcagggata gacctttgga gctttggaaa actcaatgaa
     4681 agttttaaga aaatacatga agagtctaat cagactataa tcataatttc tcaccaagaa
     4741 agaatattag agttagctga tgaaataatt gttctacaag atggttcagt aaaaagccat
     4801 ggaacaaaag aggctatatt acctgaaatc atgtgtcaag ttaattcaag ttgtgaatta
     4861 atgaaagaca tgaattaatt agaaaggaag gcaaattaaa tgaacaaaat agatttaaat
     4921 cttttagaaa aagttgctga acttcgtgga actccattag gagcatacaa tatacgtaaa
     4981 aatgggcaag gagtagagag acattcaact gaaaatgtaa ctataattcc taaaactgat
     5041 aaacctggaa tagatattat agtaaagcca aatacaaaag gagaaaatgt acatatacca
     5101 gtaatagtaa cagaggctgg tgtacaggat atggtatata atgattttga aattggagaa
     5161 aatgcagatg ttgttataat tgctgggtgt ggtatacata actgtggttg tgatgatagt
     5221 gaacataatg gtatacatag atttttctta cgtaaaggag ctcgcctaaa atatgtagaa
     5281 aaacattatg gtgaaggtga tggagaagga aagagaattt taaatccagt tactattgtc
     5341 aatatggatg aggattcata ttgtgaaatg gaaacaattc aaattaaggg tgtagattca
     5401 actaaaagag aaatggatgc aaacctaggt aaaggagcta aaatagttgt atttgagaaa
     5461 ttattaactc atggtaatca agttgcagaa tctaatatga aggttatctt agagggtgaa
     5521 aactcttctg ctcaagtagt ttctcgtaca gttgggcaag ataattctaa gcaaaagttt
     5581 aatccttgtg ttattgggga ttccctttgt tctgcacatg ttcaatgtga ttcaataatt
     5641 atgggaaatt ctcaaattag tgctatacct gaaattgctg caaatcatcc agatgctctt
     5701 ttagtacatg aagcagctat tggaaagatt gcaggagacc aaatactaaa gatgatgaca
     5761 ttaggtctta ctgaagaaga agccgagcaa caaatactta attgcttctt aaagtaaatt
     5821 aaagatttta tttaatttaa tataattaat atagtataat atagtgaata agtctaaaat
     5881 atttttattt ttaggcttat tttctttatg taagaaaagc tattagagaa attatattgt
     5941 aattaggatg tggaataaat gaaaaattta cttataagaa atgggacaat aatatctcca
     6001 acagatggta tcaaatttga aaaaagagat atcttggttt tacatgggaa aatatatgct
     6061 attggattga accttaaaga gaaaataaga aaagaaaaat tacttacaaa taaagagttc
     6121 gagataataa atgccgataa tatgtatatt tccccaggat ttatagatgt tcacacccat
     6181 tgttataaga gaaaattacc tacaggtatg gattctgatt caattggaat tgaaaaaggt
     6241 tctacagtta tatttgatgc aggtacagct ggtccctcaa actactatga ttttaaaaaa
     6301 aagtatatgg atgagtgtaa aacagaagta tattcttttt taaatgttac aaatgaggga
     6361 ctaaatatat tgaatgaatc aacttcttta gatgttataa attttgacaa ggttgagtca
     6421 attgtagaaa aaaatataga aaagataaag ggtataaaag taaaagcgag taaatttcaa
     6481 tctaatgaaa gtggtataga tataattaag aaatcaaagc aagttgctaa aaaattaaat
     6541 ttacccttag tggtatatat aggagagtat ccatcatata tagatgaagt aatgaatatt
     6601 ttgggaaaag gagatgtggt tacaccagta tatggaaata gtacaaatgg aatccttaaa
     6661 gaatctggca cattaagaaa atcagtaata aatgcaaaga atagaggagt attgttcgat
     6721 gtatgtcatg gagtggacca atttgatttt aaagttttta aaaaagcaat aaagcaaggt
     6781 ttagagcctg acctaataag tactgatatg catataaaaa acatgaaaga cgtaaactat
     6841 tctcttttaa atgttataaa taaaattatg gaacttggga tatccctaga aaagtgtata
     6901 gaaaaagtaa ctgaataccc aacaaagatt ttcgatttac atggaaataa aggcagaata
     6961 aagattggag gagaaggtga ttttacaata ttcacaatgg aggagtgtaa tgatattata
     7021 aaagactaca ataataatga acttgtttta aataaaaaat taagattgca atatactgta
     7081 aaatcatgga tgaaaagcag tgaagtttat agacatggat acgaaaatac tataataaac
     7141 taaacaatag tgataaaata ataaatatat gtttaaattg gtatatattg gataaatatc
     7201 taaggaaaca acaattaaaa aataataatt ttaggggagg tattttagta tgaaagcatt
     7261 tgttgataag gatgtatgtg tagcttgtgg agcatgtata ggaacatgtc cagacgtttt
     7321 tagtatgagc gatgaagggc attcagtagc aattgaagga gaaataccag cagatttaca
     7381 agatttatgt aaagaagccg aagaaggatg tccagtttca gctataacag ttaaatagag
     7441 ctgtttaaat ttatttttaa aataaatata taagatttaa ttgggtactt aagctatagt
     7501 attagaaatt tctattacta tagcttttta aaatagataa acttaatatg taagatatta
     7561 ctttttacaa atatggagga ctgaaatatg aaaaaaaatt atgaaatagt attattcgat
     7621 ttagatggaa cacttacaga ccctaaagaa ggaataacaa agtctattca gtattcttta
     7681 aatagctttg gcataaaaga agatttggaa aacttagacc aatttatagg accaccatta
     7741 catgatactt tcaaggagta ttataaattt gaagataaaa aagctaaaga agctgttgaa
     7801 aagtatagag aatattttgc tgataaaggg atatttgaaa ataaaattta tgagaatatg
     7861 aaagagatat tagaaatgct ctataaaaat ggtaaaatat tattagtagc aacttctaag
     7921 ccaacagttt ttgcagaaac tatacttaga tattttgata tagatagata ttttaaatat
     7981 atagcaggta gcaatcttga tggtacaaga gtgaataaaa atgaagttat acaatatgta
     8041 ctagacctat gtaatgtaaa agataaagat aaagttatta tggttggaga tagaaagtat
     8101 gacataattg gagctaaaaa aataggtata gattctattg gagtattgta tgggtatggt
     8161 tcttttgaag agcttagtga aagtgaacct acttatattg tcgaaaatgt agagtctata
     8221 aaagatattt tgttataaat atcttttata gacaaaaata ttacaaaatg gtgtcgcata
     8281 tttaaaaata tgatattata gttaaggaag tcatttaaaa aagaggagga tgcacttaaa
     8341 atgaaatcta ttttaattat gggtggaagc gacttcatag gttctgcact tgctaagcgc
     8401 ttgattaaat gtgggtatca aattgatata cttacaaatg gtaaaaagga aatagattat
     8461 aatggtttta aagaacatct aatttgtgat agaaaagttc gaaaagatat ggaaaacatt
     8521 atcacaggaa gaaaatatga ttacatatat gatatgacag catatacaaa agaagatgta
     8581 agtaatctga ttgattttat aagcatggat aacttaaaaa agtatatagt attgtcagca
     8641 ggagcagtat ataaagatag tggaagaaat ataaaagaag aaaatgagaa aggtgaaaat
     8701 gaaaactggg gaaaatatgg gttaaataaa aaagaggctg aagattttat tataaatagc
     8761 cctataccat atataattat tagaccaact tacatatatg gtgaaaataa taatttatat
     8821 agagagtatt atttctttga aaaaatagaa aaaaatgaaa agataccagt accaaaagga
     8881 aaacaagtta gtaatcaatt tatatatatt ggggatttgg taaaggtatt ggaaagcata
     8941 atgaaaaatc ctcatgtaag agaggcctat aatgtgacaa atccacagtt aatttcttgg
     9001 gatgacctta tatatacatg tggagaaatt attggcaaag aacctataat aaaatatgta
     9061 gatatggaaa aagtagagtt tagagaaaga acgtatttcc catttagaaa tatagacttt
     9121 aatcttgata taaataagct tattgaacat gggctttata taccaaatgt gttactaaaa
     9181 gaaggtctta cagcaacata taaatggttt tcagcaaata agccaaaaat gcatgacaga
     9241 aaaatgaaca aagttgagtc tgtaatgcaa atagtttaaa aaaataaata agtaggtaga
     9301 tattttgaac tcaaaaatta agtatttttt aaaatttttt atcttaatat ttattttgat
     9361 ttttatatac tcagttgcga tagagccgag tctattaatt gtgaaaaaat ataatataag
     9421 tgaaaatgga gcattgataa aaaatgatac aattgagagt aaatctgaaa gtgtaaaagt
     9481 agttcaaatt tcagatactc aaataggtag tttttattca actaagaatc tcaaaaaagt
     9541 agctaataag attaacactt tgaatccaga tataatagta tttactggtg atttaattga
     9601 ttactccaat aaaaatccaa gtgtagatga aattacaact attttatcaa gtatgaatgc
     9661 taggttggga aaattttcag tatttggcaa tcatgattat atgtataaac ttcctaggta
     9721 ttataggcag attatgaaaa attccaactt caatctttta gtaaatgaaa ataaaaaaat
     9781 aaaattaaaa gatgataaat atattaatat attaggtgtt gatgaaatat taaatggaaa
     9841 tcctaatata aaatatctgg agagtcagat agataataaa aattttaatt tattgttagc
     9901 acatgaacca gatttagtag atatgcttag taaagataca atgaatttag ttctatctgg
     9961 gcatagtcat ggtggtcaaa taagattacc tattaaggga gcattagtta ctcctccata
    10021 tgggaggaaa tatactaaag ggttttatga tataaatgga aatcatttat atgtgagttc
    10081 gggtcttgga agtacaaagt taccatttag attttttaat ataccagaga taatagaatt
    10141 taatattata atctaacata aaatatttag aaaatataat tttgcttttt ataatatgta
    10201 gatgtatata ctgtaaaaat ggattaaacc agtagattga atgaattaat atattaaatt
    10261 aatatataaa atatttaagt tataattaat gtattagaca aaataaatac tattaagaac
    10321 tattaaaata tagattaaag ataataacat ataataaata aaaaagtaag attgtgtaga
    10381 ttatataaag attatttata attaatacaa tcttgctttt ttatttaaaa attttaatta
    10441 ttatttaaaa ttttttatct tctctttatc ccatataaac aatttctcag aattatttaa
    10501 agcgccaaat acatttcttt taaaatcagg catctcttta tttaaattag caataagatt
    10561 ttttatgtac tctctcctag tatgaccatt tgcagggcag ggatttgtga taataggata
    10621 gttagaatct tttgaaattt tcttagtcat atactcttct atgtaaatca taggtcttat
    10681 aactgttaaa tcctgtctat ctaggtatgt ctttggggaa aaacaattta ctctaccctc
    10741 ataaaacata gacattaaaa atgtctcaat tgcatcatct ttatgatgac caagagctac
    10801 tttattacat ccaagttttt tagcattatc gttcaaagca cctcttctta aattagcaca
    10861 taatgcacaa ggattttttt cttgtcttat atcaaataca atttctttta tgtcagtttg
    10921 aaattcataa aaaggaacat ttatttcttt acacaattta tctagtggag aattatcaac
    10981 acctccagta tttaatgtta tggctatcaa ttcaaagttt tgtggagaaa atctttgata
    11041 acttttaagt atatgaagta gagttaaact atcttttcca ccagaaagtc caaccgcaat
    11101 tttatcattt tcttgtatca tatcaaaatc ttgtatagcc ttacgagctt tacttagaag
    11161 tttttgtata gacattttat caccatcctt taaaaatatt aaatttaaga tttatttaag
    11221 atttacttat aaaatactta agtcttatac tctaaaatta acattataga caaacataac
    11281 aaaaaggaga agatttaatt atgaaaaaat ttaaattaat aggagccatt attttagtag
    11341 tatttataag tgtatttata tctaaagata gattacttaa gaaagataaa gatccaatag
    11401 tagctgctca acatgattca gaacaagatg ttgaggcaaa ttctcaaaat aaaaatcact
    11461 caaatagtat atcaaatata ttattggtaa acaagacaaa tggaattagc aaaaactata
    11521 ctcctgaaaa tattactaag gtaaacatac cttttgttga agaagcaaca gaggaagaaa
    11581 aacaaatggc aggagaacct gcaaaggcag ttgaagattt agttaagcaa gctaattcag
    11641 aaggaataca gtttttaggg tcttcagcat acagatctta tgatactcaa ctggatactt
    11701 atacaagaag ggtaaaatct cagggacgag aaaaggcaga tgcatatgta gcaaagcctg
    11761 gttacagtga gcaccaaact ggactttgta ttgatttaac aaatccagaa agatggtttg
    11821 ttggaagtac aaaagaagct aaatggttgg cagaaaatgc tcacaagttt ggatttataa
    11881 taagatatcc tgaaggtaaa gaagatataa caggtacagc atatgaacca tggcatatta
    11941 gatatgttgg taaggatgct gccgaggaaa tttattcaaa aggattaact ttagaagaat
    12001 atttacaaaa taaataatat aaatgtgtaa ataatttgta ttatattgga aatttagaaa
    12061 tgaataaatt tatagattac actaactaaa atatagtata attttatatg aataaagcta
    12121 tatacaaaga ttaagttagg atgtgtaaaa atgaataata cagttcttgt tgtggatgat
    12181 gaaaaggaaa taagagacct tatagagata tatcttttaa atagtggata taatgtaatt
    12241 aaagctgaag atggaaaaga ggctctagat attttaaaaa aggaaaatat tcatttaatg
    12301 atattggata taatgatgcc taaaatggat gggatagaag tttgtagaaa agttagagaa
    12361 agtttgaata taccaatact tatgttaagt gcaaaatcag aagatatgga taaaatacaa
    12421 gggataatga ctggtgcaga tgattatttg acaaaaccat ttaacccact tgaacttact
    12481 gtgagagtga aatcactgat tagaagagca tattatttta gtggtgctaa taatgaagat
    12541 aaagatgtca taaaggttgg agtagtaatt atagataagg aaaaacatag tgtaacagtg
    12601 gctggaaatg aaataatttt gacatcaaga gaatttgata tattatattt actagcaaat
    12661 aataaaggca gagtatatag tacagaagaa atatttgaaa aggtatggaa agagaaatat
    12721 taccaatcta ataatactgt aatggttcat atgagcagaa taagggataa aattgaaaaa
    12781 tatacagatg gagaaaaaat aattcatact gtatggggag taggatataa aattgaaaaa
    12841 taaaagttta ttaaatcata tgtttcgacc catattagat cttataataa gctgtgcttt
    12901 aacttcaatg cttattttaa caagtatgta tataataaaa ttattatatt tcaatagtga
    12961 gatatctgta attaatcaac ttgtatatca gttaagagat ttcagaagtc ttataggaga
    13021 gagtttattt tatccatttt tatttacaat cacatcatta gcaatttatt ccatgatatc
    13081 gtacaagaga aataagcaaa ttgtagattt tataagaaag actaatagaa aaaatcaaaa
    13141 attagaaatc attaaaagtg ctgtaaattt aatggatgaa ggtaacttag aaaaaagtat
    13201 agatattgag tatgatttag atatattaaa ggataaagaa aaggatgata ttgatgaact
    13261 agcacataaa atcaataata tagtccatca acttagaaat ataactattg aagagagaca
    13321 agctcagcag acaaaaacag atttaataac aaatgtgtca catgacttaa gaacaccttt
    13381 gacatccata atgggttatt taggagtgat agaagaaggt aagtataaag atgaagttca
    13441 gatgatgtat tatgttgata ttgcatatga aaaatcgaaa aatttaaatg ttcttataaa
    13501 tgatttgttc gaattgacta aaatgcaaaa taatacaata aaactaaaca aaatagaaat
    13561 aaatttagta gagttattaa gtcaagtagt atctcaattt gaaatttatt ttaaacaaga
    13621 atttatggtt agtagaatta attttaatga agaaaaatta attgtgaaag ctgatccaaa
    13681 taaattggta agagcatttg aaaatcttat aactaatgct gtgaaatatg gaaaagatgg
    13741 gcattatgta gatatagtca cagagaaaat ggaagatatg gcggttgtaa aagtgataaa
    13801 ttatggagaa ccaattccag tgttagattt accaaatata tttgatagat tttatagagt
    13861 tgaaaaatcg agaaatagaa atgttggagg ttctggattg ggtcttgcga ttacaaaaaa
    13921 tattgtaaat ttacatgatg gagaaattac tgtttcaagc gataaatatc aaactgtgtt
    13981 tgaggtacaa ctgccaataa tacaatcagc ttagttataa aataaaagta cgattatgtg
    14041 atataaatat tataacaata aagtgtttac aaaaataaaa aagtgatata taatttaagt
    14101 cagtttaata agatacttat caagaattag gggagagagt tagctcagtg atcctatacc
    14161 aaccttttat caggtggtcc tgctaacaac gatgagatga cttgaaataa tatactcttc
    14221 ttatcaataa tagataggaa gagttttttt tagaattaat gtaacaaact ttaaagtata
    14281 atggaggatt aaataatgga aaaagtagaa agttttaagc tagaccatac aaaagtaaaa
    14341 gctccttttg taagaaagtg tagtgtactt gatggagtaa agggagataa agtaactaaa
    14401 tttgacttga gatttttaca gccaaatgta gaaagttttg gaactgctgc tatgcacggt
    14461 ttagagcatc ttttagctac ttatttaaga gatacattag atggtgtaat agacttatct
    14521 ccaatgggat gtagaacagg attttattta atactatggg gagatgtaga tgccaaaaca
    14581 gttaagattg gtttagaaga agcattaaaa aaagttcttg aatcagataa aatgccagca
    14641 gctacagcca tagaatgtgg aaactacaga gacttatctt tatttggggc aaaggaatat
    14701 gcaaaagatg ttttagataa aggattttct ttaaatatat atggagaata atttaattgg
    14761 aagagaagat ataagcataa atcaaagtga agttttaaga tatttacaat acagaggtca
    14821 agagatagat aaagacctta aatatactat tgatgaatgt gtagctataa ctaaaaataa
    14881 aataaatcct agatatttat ctaggattta tcctattaga ataaataaag ataatgttgt
    14941 agaattagag ggaacaaatt taacattaga gagtaaagat gtatatgaac ttttaaaaga
    15001 atgtaatgag tgtgtaatta tggcagctac tatagggata gatatagaaa aagaaataag
    15061 acgacactct tattcaaatt taacaaaagg actaataatt gattcatgtg gaacaacagc
    15121 aatagaagaa gtgtgtgaca ttgttcaaaa taatttagaa aaggtttttt taaaccaaga
    15181 taaatttata actatgcgat atagcccagg atatggagat ttaccaatag aaaaaaatat
    15241 agatatatta aacgttttga atgctcaaaa acaaataggt ctaacaatta cttctaatgg
    15301 aattatgata ccgagaaagt cagtagtggc tattataggt atttctaata caagaataaa
    15361 taaatctaag aaagaaaagt cgtgtgaaaa ttgtaaaaac tatagaaact gtacatatag
    15421 aaaaggagcg aactcttgtg gaaataagac aatatctgaa aaataatata ttaatatttg
    15481 atggtgcaat gggaacaatg cttcaacaaa aagggttaaa gttaggtgaa aatccagaag
    15541 tatttggtct tcaaaatcca gataaactta tagaaataca tacagcatac ttagaggcag
    15601 gttcgaatgt aattcttaca aatacttttg gatgtaatga actaaaatta gatagtaagt
    15661 atactgtaga agaggtgatt gacaatgctg ttttggtggc tagaaaagct atagaaaatg
    15721 tagataatac taaaccaaga tatgtagctt tagatattgg tcctatagga gaaatgctag
    15781 agcctatggg tactttaagt tttgataatg catatgagat atttaaaaga caagtactac
    15841 aaggcgttaa atctggagtt gatgtaatag taatagagac tatgatggat ttatatgaag
    15901 ctaaagtagc tgtattagca gcaaaagaaa actctgattt acctattttc tgtactatga
    15961 catttgatga aggtggaaga agttttactg gatgtatgcc agaatgtatg gtggctacta
    16021 tagaaggtct aggtgtagat gcaataggag ttaattgttc attaggacct aaacaacttt
    16081 taccaatagt tgaaaaaata gcatcaagag ctacagtccc tgttatggtt caagcaaatg
    16141 caggtcttcc aaatatagtt gatggagagg ctatatatga tgttgatgca aaagaatttt
    16201 ttgaaggggt taaaaagttc gttgaagttg gtgctactat aataggtgga tgttgtggta
    16261 cgaatccaag ttttataaaa gaaatatctg agaatataaa ttctgttaca aagggatgta
    16321 tcgagaaaat tgataaatgt gtagtctgtt caccatcaaa atttgtggaa gtacaatcgc
    16381 ctacagttgt tggagaaaga ttgaatccca caggtagaaa atctctacaa gaagcattaa
    16441 aaaatgaaaa tgttgattat gctataaatt taggcttaga acaagtaaat gctggagcac
    16501 aaatacttgg agtaaatgtt ggtcttccag agattgatga aaaaaaacta atgccaaaat
    16561 taataagaga gattcaagca gtagtagata cacctttaca agttgattct tccaatgtag
    16621 aggccttaga gcaaggactt agatattata atggtagaac aatagtaaac tctgtaaatg
    16681 gcaaagagga gtctttagaa agtatattac caatagttaa aaagtatggt tcatgtgtgg
    16741 ttggacttac acttgatgaa aaggggatac ctaaaaaggc tgaagaaaga tttgaaatag
    16801 caaaaaggat agtcaataga gcagtttctt gtgggataaa accaaaagat atatttatag
    16861 actgtttatc attaactgtt tcagcacaac aagaagaagc aatagaaact ataaaagcta
    16921 taacaatggt aaagacacta ggagtaaaaa ctatattagg tgtttcaaat atttcatttg
    16981 gtcttccaaa taggaaagca cttaatgctt cttttctaac attagcttta ggggcagggc
    17041 ttgatttagc aattataaat ccaaatgaat atagtatgat ggaagctatc aattctttta
    17101 aaatattaaa taacacagat aaaggatgta taaactatat aaatcaatat agtaatataa
    17161 ataattcaaa aaaatcggat tcatctacaa agattgacaa agatttgcca ttggatattt
    17221 tagttgaaag aggattaaaa gatgaagcaa aaaatgtaac actgaatctt ttaaaagaga
    17281 aggatgaaaa ttacatatta gatgagattt taattcctgc tcttgataaa gtaggaaaaa
    17341 gatatgatag cggagatata tttttaccac aattgattca atcagcagaa acggtaaaag
    17401 tttcattaaa tacaataaag gaaactttat taagtaaaag tagcaataat gtatccaaag
    17461 gaaaaataat agttgctaca gtaaaaggtg atatccatga tattgggaaa aatatagtaa
    17521 aaataatgct agaaaattat ggatatgagg ttatagattt aggtaaagat gtccctattg
    17581 aagaagtagt ggaagttgct aaaaagagaa atataaaatt agtaggatta agtgcactta
    17641 tgactacgac tgttcaaagt atgaaagata caattcaggc actgagagat aatgagataa
    17701 atgcaaaggt atttgtagga ggtgcagttt tgacagaaga atacgctgaa gaaatgggcg
    17761 ctgattatta ttcaaaggat gcaaagtctg ctgttgaaat agcaaagtta aatttttagc
    17821 atataaatta aaataaaggt tttactaaat aggatatttt atatataata taaatataag
    17881 ttttagatac ttatgtttaa aacaatagga attaatctat aataatgaga ttaattaata
    17941 taaaaaatat ttatcatcaa cattaaaaag ggctgggaag tctggtataa cttccattag
    18001 ccctttttag ttaaaatcat atgttaaaaa gcccaattac catttttaaa aatctgtatt
    18061 tgttttccat cataagtctc accaataata tccatatcag cagaaccaat cataaaatct
    18121 acatgtgtta aactgtcatt tacaccatgt tcatctaatt cttcatcttt taaattgttt
    18181 ccaccttcca gacaagtctt ataagcagaa ccaattgcaa agtggcaaga agcattttca
    18241 tcatatagag tattataaaa aattatatcc gtattggata ttggtgagtt gtatggaaca
    18301 agagcaactt ctcctaaata atgagagcct tcatccgttt caataagttt ttctagtgta
    18361 tctaatccct tttcagcaga gaaatctatt attttaccat ctttaaatgt taagaagaaa
    18421 ttgtcaatta tgtttccacc ataaacaaga ggttttgtac tataaacagt cccatttact
    18481 ttaaacttat gtggcattcc aaaaatctct tcagttggaa tattcggatt aaaacttatt
    18541 ccattagggt ctttggatgc tccacttagc caaacatgac cctttggcaa gtctagagtc
    18601 aaatcagttt ttgaggattt atattttaaa gttttaaagt tatttttatt tagaaagtcc
    18661 attttagatt taagattttc attatgttct tcccatgctt ttatagggtc ctgaccatct
    18721 actcttgaac atttaaatat agcatcccac agtttttcta ttgctttatc ttctgataaa
    18781 tttggaaaca ctttttttgc ccaatctttt gttggcacag agactatact ccatttacat
    18841 ttatcagtta gagtgtaaga acgccattct tttaaagctt ttccagatgc cttctgaaaa
    18901 tttgcaactc tttcagggtc aacatctttt aataaatctg gattttgggc atagactgat
    18961 aaaaatgccc caccttcttt tgctatatct acatattgtt ctgctgtcca tttggggaac
    19021 tcatgaaatg cttcatctgg agcatacaag tatttaataa gagtactttc ttcatcagac
    19081 cactctatat aaacattttt agcaccacat ttatatgctt ctttaagtgc atttcttgca
    19141 aaatcagcac attctattgg tgactttata agtagaattt gacctggctg tatgttaact
    19201 ccaactttta ttgctaattc agcgtatttt tctaaatttc tatttaaatt catatttatc
    19261 ctcctatatt tttaatattg aataacttat aaagtattat aacattactt atgtttttaa
    19321 tattgaacaa cttataaaat attataactt tacttagatt ttgggtcata ttcaaaatat
    19381 ttatacttta tagattgaga aataaaaaaa atattttaat taaaacagat tatgaggata
    19441 acaaacttat ttaagcagta tgttttttgt tgataataat attaaagtag aaataaaaat
    19501 acttttttag tgaaaagcta tacaagaata ccgaaaaaaa cttataatat aataatgaga
    19561 tagatattta taaatgaaaa aaatttttct ggcaaagctt aattaaaaag gtaatagcta
    19621 gtgttgttat ttaaattagc atagaaaatc aaataaaaaa attatgctaa tgtatagaca
    19681 ttgataaggt agaatttaag ccttttgaat gctatattaa actagagtag atattcttat
    19741 gaatttcaaa atagggcagg aggaaaaagg aaataaaact ataaaaaata taaagtattt
    19801 atataaattt atagagttta tacgtcagaa gatttcagtt aggagggcat aaatggagct
    19861 ttatgaagcc attttttata gaaaatctgt aagaaatttt tccagtaaga agataaaaaa
    19921 gccattgatg gaagaaataa aaagaagttg taaaaattta gaatacttaa atgaagattt
    19981 aaatattaaa gcacatgttg ttgatagagg tcatataata cattttttga tgggtaagga
    20041 atgtaaagta aaggcacctc attatatagt agttacatca gacaaaggtg aggactattt
    20101 acagaatata ggatttgcta cagaaggtgt agttttgcgc cttacaacat tgggaatagc
    20161 tacttgctgg ttggaaggaa acttaaagag agaagatatc ttggaatttg tagatattgg
    20221 gaataagaat tttgaagaag atgaggaaga tgatttagat tatctagctg gagtaaaaac
    20281 agaagaagat gatgaaaaca aaatagaaaa tccttatata ataatagcat ttggatatcc
    20341 agcaaaaaat gaagagttgt ttaggacgag atatgctaga cctgatagaa agccagtaaa
    20401 agatatgata aataagataa ataggaatag aaagtggaaa gatatcttag aattgacaag
    20461 aagagctcca tctgtaaaaa attctcaacc gtggtatttc cataaggatg aacgaggtct
    20521 tcatttattt gaaaagagac ctaagaaaca ctgtgaagat atgaataagg ttagcttagg
    20581 tgtagcctta agacattttg atatagcttg tataaaaaat aaaatagatg tttcatatga
    20641 aaaacttcct ataaggaata aaataggaaa atcatatttt ataactgtag tcgagcatgt
    20701 gaaaccagaa gaagaaaccc aagaagaaaa tgtaactcta gaaaaagaag aatcacaaga
    20761 tgaataaaat tttggaaggg gatattggat atgtataagt gtggaaaatg taatcttaga
    20821 ggctgttcta atggaaatat ggataaatta atgaagatat gtccaactag agaaattgaa
    20881 ctacaacaaa aagcaaagca actttatgaa gaagaagaga atagaaaaat agcttataat
    20941 gctggattag ttgaagcaga ggggtattgt caatatacta gactaaaaga gattatagag
    21001 tttgctaaaa agtgtggtta taaaaagatt ggcatagcat tttgtattgg actaaaaaga
    21061 gaaatgcaaa cagcacaaaa aatatttgaa catcatgggt ttgaagtatt ttctgttata
    21121 tgtaaaaatg gggcaatgcc taagagtaca attggactag aagcatctca aacaatatca
    21181 aagtgttcag atgaggtaat gtgcaatcca ataggacaag ctttattttt aaatgaagct
    21241 aaggttgacc ttaatgtaat tcttggatta tgtgttggtc acgatacttt agccatgaaa
    21301 tatatggaag ctcctataac aactttagtt gtcaaagata gagtaactgg gcataatcca
    21361 gttgctgcaa tatataatgc agaatcatat tttgagaaaa aattgtttga taaagatgaa
    21421 gaataattca aagacttatt gacataaaaa gcaattagtg gtttaattat aaaaaagata
    21481 aaactttaaa ttatcctata gatacataaa gttatataaa taaagaaaaa tattacagct
    21541 aaattcctta aagtagtcca gagagactaa agggttatag ataatgttag tatataacct
    21601 ctagtaaatc ttctagaggt ttttgatgag tataaaataa aaatttaaat ataataaagt
    21661 cttttaaact agtccagaga gactgggaaa gatatctcat agctaatgaa tggagttatg
    21721 tatagataaa atccttatgc ctcttttggt gtgaggattt ttttatgatt atttaggctt
    21781 tattaaaaag gagatgtaaa aatgataaat ggtaaagaaa aattgatagt tgcaattgat
    21841 actgacgaat ttgataaagc taaagaacta atagacagat tagaagatag tgtagacata
    21901 tttaaggtag gtctagagca atacgttgcc acaaaaggta aaacaataga ttatcttaaa
    21961 gaaaaaggga aaaaaatatt tttagacctt aaattccatg atataccaaa tactatgaaa
    22021 agtgcagtga gagctgcagt gagagataat gtttggttaa tgacaataca tgtatcagat
    22081 atggaaggaa tgagacaatg tgcactaatt gctagggaag aagcagaaag attaaatata
    22141 gaaaagccat tagtagttgg agtaacagtt ttaacttcat taagtaacca agatttacag
    22201 gacataggat gtaatatgac aacagaagag ttagctataa agagagctaa actggcaaaa
    22261 gaatcaggca tagatggggt tgtttgttca gcgcaagaag tagataaaat agtagaagca
    22321 tgtggagctg attttataac agtttgccca ggaataagac ctaaatcagc agaggttgga
    22381 gaccaaaaaa gagttgtaac tccatcagat gcaataaaaa aaggtgctca ttatttagtt
    22441 gttggaagac ctataacaaa agcagaaaat ccaagagaat cagctattaa tattgtaaga
    22501 gaaatagaaa atgcttagat aaaatatttg ggaggtagct tatatgaagg ggaaattaat
    22561 tttagaagat ggaacagtat ttgaaggaaa agcatttgga tatctaaagg aagctattgg
    22621 agaagttgta tttaatactt caatggctgg gtatggagaa atattgacag acgcaactta
    22681 ttatggacaa atagttacta tgacttatcc acttatagga aactatggaa taaatttaga
    22741 agatgttgaa tcaaataagg tgcaagtaaa aggacttatt gtaagagaaa aaagtgataa
    22801 cccaaataat tttagatgtg aaatggatat agacacatat ctaaagcaaa ataaggtaat
    22861 aggacttgaa ggaatagata caagagcatt aactaaaatt attagaaaca atggaactaa
    22921 gagaggaatt ataactttag aaaatgtatc attacaagat gttcagtcta aattagaaga
    22981 gcattgtaac aaagattcag taaaaaatgt tacaagaaaa gaaattgaaa atataaaagg
    23041 tactggagct aaagttgtag ttatagactt tggtgttaaa aatagtgtac tagagtctct
    23101 taaagcttgt aactgtgata taactatagc accagcaatg actacagtag aagaaatttt
    23161 aaatataaat cctgacttaa tcttattatc taatggacca ggaaatccag aggatataaa
    23221 tgatatagtt gaaaatataa aaaaattaat aggcaaaaag ccattgacag gaataggttt
    23281 aggacatcaa attttagcat tggcattagg aggaaagact tcaaagcttg tatttggaca
    23341 tagaggaggt aatcacccag ttaaatgttt agaaactgga aaggtattta taacttctca
    23401 aaatcatgga tataatgttt cagaaatgcc agaaaataca gaggtaactc acataaattt
    23461 aaatgacaat actatagaag gaatgagaca taaggaatta ccaatatata gtgttcagta
    23521 cattccagaa tacacaacaa aagaagattt agattgtata tttaataaat tcttagcatt
    23581 aatatagatt tagtattatt tacagatagt aaaaaccatt tagataagga gaaaaattta
    23641 tgcctaaatt agatagtata aagaaaactt tagtattagg gtcaggacca ataataatag
    23701 gtcaagctgc agagtttgac tattcaggaa cacaagcttg ccagtctttg aaagaggaag
    23761 ggattgaagt tgtattaata aatagtaatc cagccactat aatgactgat aaagaagtgg
    23821 cagataaaat atatatagaa ccattaacaa tagaatttat agaaaaaata atagaaaaag
    23881 aaagaccaga cagtttactt gcaggtatgg gtggacaaac agggctaaac ctagcagttg
    23941 aactttatga aaaaggcata ctagataaat atggagtaaa gataataggt acttcagtag
    24001 aatctatcaa aaaaggtgaa gatagagata tatttagaga agtaatgaaa gaaataaatc
    24061 agcctgtaat agttagtgat atagttacag atttacaagc aggtcttgat tatgcactta
    24121 caattggtta tccagtagtt gtaagaccag catacacact aggtggaact ggtggtggta
    24181 tagctgataa tgaggaagaa ttaagagaaa tactttcaca tggtctacaa ttaagtcctg
    24241 ttggacaagt attaattgaa aagagtataa aaggttggaa agaaatagaa tatgaggtaa
    24301 taagagattc aaagggaaat tgtatagttg tatgtaacat ggaaaatata gacccagtcg
    24361 gagttcatac aggagacagt atagttgttg ctccaactca aactttaagt aacaaagaat
    24421 gtgcaatgct taaaaaagca tctttagata tattaaatgc agtagaagtt caaggtggtt
    24481 gtaacgttca atttgcactt aatccacata gttttgaata tgcagttata gagataaatc
    24541 caagagttag tagatcatca gctttagcat caaaagctac aggatatcca atagctaaag
    24601 tttcatcaaa aatagcttta ggttatactt tagatgagat agaaaatgca gtaactaaaa
    24661 aaacttatgc ttgttttgaa ccaactatag attatgtggt ggcaaaaata ccaaaatggc
    24721 cttttgataa atttaaaaaa gcaaatagaa aattaggaac taagatgatg gcaactggtg
    24781 aaattatgag tataggaagt aactttgagg ctgctattct taaagggata agatcacttg
    24841 aaacaggaaa atattcatta gttcacacac catctgagga gagaagcatt gaggaattaa
    24901 agaaaagagt tgtagtacct gatgatgaga gactatttga cttagctgaa atgataagaa
    24961 gaggctataa agtagagatg atagaacaaa ttacaggtat agataaatgg tttataaata
    25021 aatttaaatg gatagtagag caagaagaaa aattaaaagt tatgaaaatt gaagatttaa
    25081 ctaaagacta tcttcttgaa ttaaagaaaa agggattctc tgataaaggt atatctgact
    25141 taatgaagat aagtccagaa aaattatatg aattgagaag tctatacaat atcaaaccag
    25201 catataaaat ggttgatact tgtgcaggtg agatagatgc tatatctcct tactattatt
    25261 caacttatga gcaatatgat gaggtagttg ttagtgataa gaaaaaggtg atagtattag
    25321 gttctggtcc aataagaata ggtcaaggta tagaatttga ctattgttca gttcattgtg
    25381 taaaatcttt aagaaaaatg gacatagaaa ctataatcat aaacaataac cctgaaactg
    25441 taagtacaga ctttgataca tcagataagc tgtattttga accattaaca gaagaagaag
    25501 ttttaagtat aatagaaaaa gaaaagccag aaggtgtaat actacagttt ggtgggcaaa
    25561 cagctataaa attggctaag tttttacatg aaaaaaatat acctatactg ggaacagatt
    25621 ttgatgatat agatgcagca gaagatagag aaaaatttga tgatttacta gagagactag
    25681 atataaatag accaaaagga aagggtgtat ggtcacttaa cgaaggtatt gaagttgcta
    25741 atgaattagg ttatccagta ttagttagac catcttatgt acttggtggt caaggtatgg
    25801 aaataactta taacgaagaa aaattatctc aatatctaca
//
LOCUS       DAEPLF010000032        25204 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEPLF010000032 DAEPLF010000000
VERSION     DAEPLF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.032"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            164..541
                     /locus_tag="KR314_001215"
     CDS             164..541
                     /locus_tag="KR314_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBH0327609.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            949..2754
                     /locus_tag="KR314_001216"
     CDS             949..2754
                     /locus_tag="KR314_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0327610.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIYKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNVCKNKDINIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            2831..3370
                     /locus_tag="KR314_001217"
     CDS             2831..3370
                     /locus_tag="KR314_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HBH0327611.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            3993..5207
                     /gene="dpaL"
                     /locus_tag="KR314_001218"
     CDS             3993..5207
                     /gene="dpaL"
                     /locus_tag="KR314_001218"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBH0327612.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLRDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            5540..6727
                     /locus_tag="KR314_001219"
     CDS             5540..6727
                     /locus_tag="KR314_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBH0327613.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYNGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            6743..8326
                     /locus_tag="KR314_001220"
     CDS             6743..8326
                     /locus_tag="KR314_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBH0327614.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            8354..9682
                     /gene="ssnA"
                     /locus_tag="KR314_001221"
     CDS             8354..9682
                     /gene="ssnA"
                     /locus_tag="KR314_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBH0327615.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            9881..11233
                     /locus_tag="KR314_001222"
     CDS             9881..11233
                     /locus_tag="KR314_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBH0327616.1"
                     /translation="MSQDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            11425..12534
                     /locus_tag="KR314_001223"
     CDS             11425..12534
                     /locus_tag="KR314_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0327617.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            12817..14208
                     /gene="hydA"
                     /locus_tag="KR314_001224"
     CDS             12817..14208
                     /gene="hydA"
                     /locus_tag="KR314_001224"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBH0327618.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            14202..16763
                     /gene="xdh"
                     /locus_tag="KR314_001225"
     CDS             14202..16763
                     /gene="xdh"
                     /locus_tag="KR314_001225"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBH0327619.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            16915..18261
                     /gene="rpoN"
                     /locus_tag="KR314_001226"
     CDS             16915..18261
                     /gene="rpoN"
                     /locus_tag="KR314_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBH0327620.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            19058..20080
                     /locus_tag="KR314_001227"
     CDS             19058..20080
                     /locus_tag="KR314_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0327621.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            20140..21147
                     /gene="gap"
                     /locus_tag="KR314_001228"
     CDS             20140..21147
                     /gene="gap"
                     /locus_tag="KR314_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBH0327622.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            21369..22571
                     /locus_tag="KR314_001229"
     CDS             21369..22571
                     /locus_tag="KR314_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBH0327623.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKANNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            22597..23340
                     /locus_tag="KR314_001230"
     CDS             22597..23340
                     /locus_tag="KR314_001230"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBH0327624.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLSK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            23349..24881
                     /locus_tag="KR314_001231"
     CDS             23349..24881
                     /locus_tag="KR314_001231"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBH0327625.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFANKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT        10165 a         2839 c         4968 g         7232 t
ORIGIN      
        1 attgctttaa taaaagttaa caagacacaa ataaacataa tcatctaagt attgattatt
       61 tgaagtttta gatttgaaat ttaatctaaa ggttcgtatt aatattggtg tttaggaaat
      121 tttattttcc tgtaaatata acttaataat aggaggtgaa gtcatgtcaa aagtagtaat
      181 agctacagag aaagctccag gagcaattgg accatattct caagcaattc aagttggaaa
      241 catagtttct acatcaggtc aacttccaat agatatgaag actggcgagt tagtatctga
      301 cccaaaagaa gcaacaaaag cttctttaga taatgtaaaa gcaatattag aacaagctgg
      361 tacaagtatg gataaaatat ttaagactac agtattcgta aaagatttaa aggattttaa
      421 tgatattaat gaagtatatg ctacatattt taaagaaaat ccaccagcaa ggtcatgcgt
      481 acaagttgct caattaccaa aagatgcagt tttagaaatt gaagcaatgg cagtattata
      541 agtattataa caataaaacc aataacaaaa ctaataaaaa ttaaaaacta ataaaaaaag
      601 tctacaatta aaaaagttta tattaaaaaa gtctatataa aaatctatct aaatatttaa
      661 ctattgagct tttctcatat aatgtatatt tttatgtcaa aaatatattt gttatatgag
      721 gaaagctttt tttattttat tattgtattg tgatttactt tattgtctgt atcttataat
      781 taagtaatta aggtcatcat ataaaacaaa ctttttataa attaatattg tgtgttttaa
      841 tgtaaatata taaataagta atgttttata tatattttaa agagtgacta tatgttaaaa
      901 tatagctaaa ctgataattt ggtaaattaa attctaatgg gggaaactat gttaagtaaa
      961 ttaaaagaat ttcaacaaga aatgataaaa tacactgaaa cagtagctag tgtgttagat
     1021 gtagatattg aaatagttga tgatagatta ataagaatta gtggtacagg tctgtataaa
     1081 tcaaagataa atgaaagcgt tgttacagaa ggatttattt acgacaatgt tatacagaca
     1141 ggtcaggaat tagtcgtatt ggatatatgt gataatcagc tttgtataga atgttcccat
     1201 tacatgaaat gcctaaataa agtaataata gcagttccaa taaagtataa taatcgaact
     1261 ataggagtaa ttggtgctat tagtacagat aaaactaaaa aagtagaaat aagtgcaaaa
     1321 atagataatt atttaaaatt tgtcaatcat atatgcgatt taatttctat gaaaatagaa
     1381 gaacatgaag cgtcaaaaaa tagcagtaga aaaatggata tgatgataga aatcattgaa
     1441 aatgttgaaa aaggcgttat aatactagac ataaatagca aaatatctta tataaacaat
     1501 atagctctaa aaaaattaga tatatataaa aatataatag aaaatatagt aaatatagta
     1561 tctgttgaat cttcctcaaa tggacatgaa ttactagaaa ttgatattga taacaaaatt
     1621 tataatataa atgccaaaat aattccggta tatccatata taaatcaata cgacaaaata
     1681 ataatatttg acaagacata tatcaatcat aaaggacatg gaaaagtcaa tagtggctgg
     1741 ggaaatagtg atatagagtc tataattgga aattcagagg caatgttaaa agtaaaagag
     1801 agaactaaga aattagcaaa gtcaaattca actgtactta taactgggga aagtggtacg
     1861 ggtaaagagc taattgctag ggctatacat gctgaaggta gcagatggaa caagccattt
     1921 attgcgataa actgtgctgc tattccagaa aatctccttg aaagtgagct atttggctat
     1981 ataaaaggtg catttagtgg agctagtagt ggaggtaaag taggtaaatt tgaacttgca
     2041 aatgaaggtg ttatatttct tgatgaaata ggagatttgt caatgccttt acaagcaaag
     2101 ttacttagag ttttacaaga gagaaaattt gcgagaattg ggtcaaataa acttatagat
     2161 ttagatataa gagtgattgc tgctacaaat aaaaacttat tgaaattagt aaatgaaggt
     2221 aagtttaggg atgatttata ctatagatta aatgtaattc ctataaattt gccacctctt
     2281 agggaaagaa aggatgatat tgaggcaata atgatgaagt ttgcttctaa atattcttta
     2341 gaattaggta ttcagttaaa taaaatagaa gaaaatgtaa tgaatatgtt gattaactat
     2401 aattggccgg gaaatataag ggaactggaa aatgcagttg aatatatgat gaatttggtt
     2461 ggagatgatg gaataatata taaagatatg cttccattgg atattttaaa ttattataat
     2521 attaatggaa atgtttgtaa aaataaggat ataaatataa tatttgaaga tgacatagtt
     2581 ggagggatag tagagaatca ggaaaggata ctttcaatta aagaattaga gttgacttac
     2641 ataaacaagt tgttgaataa gtatggaaga gatacaaaga cgaagaagaa gatagcaaag
     2701 gatttaggta taggtttggc tacactatat agaaaattgg aagaggaaca gtagtaatat
     2761 aatattaaaa tgataggtat ttgtgtgttt ttatagatag gtatttttac tttatggtgg
     2821 aggtgttatt atgcaatatg cgatagagct atattatgat aaaaaaacgg aaaagcaact
     2881 ttttgattta tctaaaaaaa tcgctgatga aaaaattagt acgaagtact tagaatggaa
     2941 aacaagacct catcttactt tggcttgttt caatgatgtg gatgaggctt gttgcataga
     3001 caagctaaag gggtttgcac aaaaccataa ggtaatacct gcttacattg gttcagtagg
     3061 catgtttaat gatactaaaa cggtgtttgt atcacctata atgaatagca gtatgtatca
     3121 gtttcagaga gaattatatg aggtactaca agattttgat tcaaatggtt gggaatggta
     3181 ccaccccaat cgttgggtac cccattgcac aatagcactt actggagatg atgaaagtga
     3241 ggcttttttt cgggcaagtg atgtattact gcgtgagttc aaaaaaataa gtggtgagtt
     3301 tgtctcaata ggattggtta aaattacatt tcctgtggaa gaaattttta cagtgaatat
     3361 gaataggtaa ctttaactct gttgtggatg ttatgaatat tttgcatagt gcatttcatc
     3421 agataaatgc gaagttgcga atttcgacag ctcaatagtc gcaagtagga cacaatggta
     3481 cacatattgc acttttgctt attggaggga cgcccatgta aacttctgta agaagctatg
     3541 aaaagattta agccattttg gaggctttgg gtaataaaga atatttgggt aataaagaat
     3601 ataaagtata taaatttata gagtttatgc gtaagaaggt tatagccata tatttataga
     3661 aaataaaagt agtaatatat ctgacaaaac aatagacata taaaaaataa gagcataata
     3721 aaggaagaat taaaataaat aaacgatttc tcgaattgat aattctcgta ttgataaaaa
     3781 tttcccaata tgagaatttt ttcttttata aatcttattt ttcacattta atatttataa
     3841 aattcaaaga aattttataa atatatgtaa ttccaacata aaaaaattgt ttttagaata
     3901 aataatattg gcatagtaat tgcttcacta taagacacaa agataaaaaa ttaaaatgac
     3961 accgaaaaat ttgaaagggg aatataaaat aaatgagaga aataaaatgg aaattaaata
     4021 acttacctaa atcagaggaa aaagaaaaag gaattgagtt tttaagagat gaagaaattg
     4081 caaaggctaa agcttttcat gagagctttc cacaatatga aaaaactcct ttagttaaat
     4141 tagataactt agcaaaatca ctaggcgtaa gcggaatata tgttaaagac gaatcttata
     4201 gatttggttt aaacgcattt aaagttttag gtggttctta ctctatgggt agatacttag
     4261 ctcaaagatt agatacagat atatctgagt tagggtatga caaattaact tcaaaagaaa
     4321 taaaagaaaa attaggagaa ataactttct ttacagccac agatggaaat catggtagag
     4381 gagttgcttg gacagctaat aaattaggtc aaaaatctgt tgtattaatg ccaaaaggtt
     4441 cttctgaatt tagattaaat aaaatcaaag gagaaggtgc agatgctagt ataacagact
     4501 taaactatga tgatgcagta agattagcta atgattatgc agaagctgat gaccatggag
     4561 taatggttca agatacagca tgggacggat atgaagaaat accagcatgg ataatgcaag
     4621 gatatggaac tatggctaaa gaagctatag aacaattaaa agaatatgga gtagatagac
     4681 caacacacgt atttgttcaa gcaggagttg gttcacttgc tggtgcagtt caagggtatg
     4741 ttgcttcaat atatgatgaa tgcccaataa cagtagtagt agaggctgat gaggctgatt
     4801 gttactataa atcagcagaa gctggagatg gaaaaccaag atttgttgga ggagatatgc
     4861 caactataat ggcagggctt gcttgtggag aaccaaacac tataggtttt gaagtgttaa
     4921 aaaattattc atcagcattc gtttcagcac cagactgggt atctgctaaa ggtatgagaa
     4981 tattaggaaa ccctctaaga ggagatgaga aagtaatctc tggagaatct ggagcagtta
     5041 caactggagc attagtttca atccttgaaa gtgaagattt aaaagactta agagaagctt
     5101 tgaaactaga tgagaactct aaagtattat taataagtac agaaggagat actgaccctg
     5161 ataaatatag agatatagtt tggaatggtg aatgccaaag taaataaaag acttaaggaa
     5221 aataaaaact taacaaataa atgaaaaatt taaggaaaat aaaaacttaa ataagtgaaa
     5281 actttaaata aaataaaaac taaaacaaat gaaaaatcta aataaatgaa aaagtaaagc
     5341 aaatgaaaaa ttcaaaataa aaactaaata aaattaaata aaaccaaata aaattaaaat
     5401 aaagtctaaa taaaaattta ataaataaaa tataaagaaa taaaaaatca atagattaag
     5461 tgaaatgcta ataaattaaa aaagtgaaat atcaaaataa aacgtcaaca aattaaaaat
     5521 tatatggggg aatatcaaaa tgttaaatga aacaagaaaa aaacaattaa ctgaagtatg
     5581 ccaagactta ataagaaatg ctagttattc aggtcaagaa gaaaacgtag taaaagctat
     5641 agaagaaaat tttgctttac taggttttga tagttggtca agagatagat atggaaatat
     5701 agtaggatgt ataaaaggaa ataaaccagg taaaaaaata ttatttgacg ggcatataga
     5761 tacagttcca gttccagatg caagtaaatg gagtgttcct ccttttgaag cgagaatagt
     5821 agatgggaaa atatatggta gaggaacttc tgatatgaag gggcaagtaa gtgctatgat
     5881 ggctgctgtt tcatactttg cagaagatac taacaaagac tttgaaggtg aattgtatgt
     5941 agcaggtgtt gttcatgaag aaatatttga aggtgtttca gctagagaga taagtaaggc
     6001 tgttcaacct gattatgttg taataggtga atcttcagaa ttaaacttaa agataggtca
     6061 aagaggtaga ggagaaatag tagtagaaac ttttggtaaa ccagctcact ctgctaatcc
     6121 agaaaaaggt ataaatgcag tttataaaat ggctaatgta atccaaaaga tacaacaatt
     6181 agtacctcca actcatccag ttttaggtga tggaatatta gttttaacag atataaaatc
     6241 ttctccatat ccaggagcat cagtagtgcc agattattgc aaagctactt ttgatagaag
     6301 attattagtt ggagaaacaa gagaaggcgt attagctcca atacaagctt tattagatga
     6361 aatgatgaaa gaagacccag aattaaatgc aaaagtttct tatgcagttg aaaaagctga
     6421 ttgctataca ggaaatacta tagaaagtga aagattcttc ccaggttggt tatatgatga
     6481 agaagatgaa tttgttcaag cagcgtataa tggattaaaa gaagctggaa tagattcaga
     6541 aataactcaa tactcattct gtacgaatgg aagtcactac gcaggtgagg ctggaataag
     6601 aactatagga tttggtcctt ctaaggaaaa tcttgctcat acaatagatg agtatataga
     6661 attagaccaa ttattcaaag gtgcagaagg atattatggt atattaaaat cagtttataa
     6721 aaaataatta ggagatggtt ttgtgagaac tttaataaaa catgggctta tagtagatgg
     6781 aaataaaaca ccagcatatg aaggagatat tttaatagaa aacgagaaaa tattaaaaat
     6841 atcacaagat ttaaacgaag aagctgacaa agttatagat gcaaaaggta gagttatatg
     6901 tccaggtttc atagatactc atagccattc agacctagtt attttagtta atccatataa
     6961 tgaggtaaag attagacagg gaattacaac agaagtgttg ggccaagatg gtatatctat
     7021 ggcccctcta cctcaagagc atatatcttc ttggagaaaa aatttagcag gtctagatgg
     7081 tgaaagtgat gaaattgatt ggaaatatga aacaacagag aattatttaa aaatgatgga
     7141 ttacaatggg gtaggtttaa atgaaactta tttagtacct catggaaacg ttagaatgga
     7201 agctatggga cttgaagata gacctgctac taaagaagaa attcaaaaaa tgtgtgaaat
     7261 cacagagaga gaattaaaag caggagctat aggattgtct actggactta tatatatacc
     7321 ttgtgcatac tcattgactg aagaaattat agagatgtgt aaggtagtag caaaatatga
     7381 cggagtattt gtagttcacc aaagaagtga agcagataca attttaactt caatggaaga
     7441 aatcatagaa attggaaaac aatcaggagt aaaagttcat ttttcacatt ttaaggtttg
     7501 tggtaaagca aactggaagt atataccaca agttatagag cttttagaaa aagcagaaaa
     7561 ggaaggaata agagtatctt ttgaccaata tccatatgca gcaggaagta caatgcttgg
     7621 agtagtttta cctccttggg cacattctgg aggaactgat aagttaatag aaagattaag
     7681 cgatgaaaac gaaagagcaa aaatgaaaaa agatatagca aatggaatag aaggctggga
     7741 taattttata gaatttgctg gaatagacca aatatttgta acaagcgtaa aaacagaaaa
     7801 gaataaagat acaattggta agagcttatt agaaattgga aaaatgagag gaaaagaccc
     7861 attagatgca acgtttgatt tattaaaaga agaagaaaat gcagttggga tggtagattt
     7921 ctatggatta gaagaacata taataggatt catgaaaaga gacgagcaaa atgtgtgtac
     7981 agatggtctt ttagcaggta aaccacatcc aagagcttat ggttctttcc ctaaaatact
     8041 aggaagatat gttagagagt tgaatgtact aacaattgaa gaagctgttt ataaaatgac
     8101 taaaaaggca gctacaagct ttagtataaa agatagaggc gaattaaaag aaggatattt
     8161 tgcagatata gttatatttg acaaggatac agttagtggt tgtgatgatt ttatcaactc
     8221 tatgcagtac cctacaggta tagattacgt aattataaat ggaaattgtg ttgttgaaga
     8281 aggaaaatac aataaaatta aagcaggaaa agtacttaaa aattaaatct tagctttaag
     8341 gaggaataaa tttatgattt tagtggggaa tgggaggcta ataagccaag attctttaaa
     8401 tccctatatg gaagatggat gtgtagtaat aaatgataat ctaattgaag atataggtac
     8461 tacagaagaa atgaaagcta aatatccagt acatgaattt atagatgcag gcaagaaaat
     8521 aatcatgccg ggtctaataa attcacatat gcatatatac agctcatttg ctagaggaat
     8581 ggcagttcca ggaaaacctt ctgagaactt tttagagata ctaaataatt tatggtggag
     8641 acttgacaaa cagcttacac tggaagatac aaaatacagt gcatatgcaa cgtatataga
     8701 atgcataaaa aatggtgtaa caacagttta tgaccatcat gcaagtccaa atgcaattga
     8761 aggcagtttg tttacaatat cagatgttgc aaaggatttg ggaattagaa cgtgtctttg
     8821 ttatgaagtg tctgatagag atggtggaaa tacaatccaa caaggtatca atgaaaacgt
     8881 aaactttata aaatacgctc aaaaagatga tacagacatg ataaaaggaa tgtttgggct
     8941 tcatgctgca tttacactat ctgatgatac attaaataaa tgtgcttcag agatggctgg
     9001 tcttgataca ggttaccatg tacatgttgc tgaaggacta gcagacttac aatataatgt
     9061 agataaatat ggaaaaaggg ttttagaaag attcaatgat tttggagtac taaaagaaaa
     9121 aacaatagca gttcattgta tatatataag tgcagaagaa atcagtcttt taaataaaac
     9181 aaatacattt gtagttcata atccagaatc taatatggga aatgcagttg gatgttctcc
     9241 tgcaatagag ttattaaaaa ggggagttac agtaggatta ggaactgatg gatatactag
     9301 cgatatgttt gaatcattaa aagttgccaa tataattcac aaacatcact taaaagaccc
     9361 aagagttgga tttgaagaat cacaaacaat gttatttgat aacaatagaa aaatagctaa
     9421 agcttattat aaaaatgacc taggagtact taaaaaaggt gcttatgcag atgttatagt
     9481 agttgattat ataccacata ctccattgac agaaaataca atagggggac acactatttt
     9541 tggaatgagt ggaagaagcg tagatacaac tattataaat ggaaaagtac ttatgaaaga
     9601 tagaaagtta ataaacatag atgaagaaag tattctagca aaaagtagag aattaagtca
     9661 aaatctatgg aatagaattt aaataattga ttatgcaaaa ttctatgaag caggaaccct
     9721 gtgactttag tcatgggagg ttcagattaa tatacaagta agaaacaaga atttagatgt
     9781 taaaaacaga gcttaaacaa aaataggaat ttaaatgaag tatataaata aaaaatatac
     9841 ggaatagaat attaaattaa atatagaagg gagacccaaa atgtctcaag ataaaagccc
     9901 taaaatagct ccagtagatg aaaggttacc tgtatccaaa tcttgggtat tttcactaca
     9961 gcacgtttta gcaatgtgtg caggagcagt agcagttcct atgatgatag gaagtgctgc
    10021 acgactagat catgctagta ttgttttttt aataaatgct tgcttgttta tggcaggtat
    10081 aggaacactc atacaaagtg ttggtatagg aaatattgta ggtgctaaaa taccagtaat
    10141 agaaggaact agttttgccg ctgtggcagg tatgacagca atagcctcat catatagtga
    10201 ttcacaagtt gcaatacaga cagtatttgg ctctgtaata gtagctggat tgttttgtat
    10261 attacttgct ccagtatttg aaaaattaat aagatttttc cctagtgtag ttactggaac
    10321 agttgttatg gtaataggta tttcactatt tccagttggg ataaaatgga taactgacaa
    10381 caaattggaa gcggttgaac caaactcatt agcattagct ttagcagtat tggcaataac
    10441 tttactttca tttaaattct taaaaggaat ttggaatagc acagctatat tggtaggtat
    10501 agtacttggt acactacttt ccatggcagt tggaatggca gattttagtg cagtaaatgg
    10561 agttggttgg cttaacttaa atgtaccttt aaaatttgga atgcctagat ttgaaccatc
    10621 tgcaatacta tcattgtgtt tgattatgtt ggtacttatg acggaatcag ttggtaatat
    10681 gatagctatt catgaaatgg tagacaaaga agtaacaggt aaaaatatta aaagaggtct
    10741 atttggagat ggaatatcta cagcattatc tggaatattc aattcatttc caataactcc
    10801 atttggtcaa aatacaggaa tagtaggtct tacaggtata aaatcaagat ttatagcagt
    10861 ttacgcaggg gttatattgc tagtacttgg attcttacca aaatttgcag caataatagg
    10921 agcaatacct aagcctgttc tcggaggcgt aggatttgca atgtttggaa tggtaatggt
    10981 tggaggagta aaaactttaa gcaaagtgaa atttgatgga actaaaaatg gggttatagt
    11041 agcagttagt gttggtttgt ctatgatacc actaacaaat cccgatttct atcataattt
    11101 cccaacttgg gtacaaacaa tattccatag tggaataaca acaggtagtt tatcagcagt
    11161 gcttttaaac atattcttca atgaactagg ttctaaaaaa gaaaaaaata ataaagttga
    11221 agaagcaaaa taaaaataaa acaagtaaaa atctatattg acttaaagtc attaagcatt
    11281 aataaaaata attcagagat attaataatt caacaaaact atcttatatt ttaaataata
    11341 taaaaaagta aataaataac gaatacaaat aaaagaatac aatctaaata ttaaaaatat
    11401 ttaatttaag ggagagataa taatatggct aatttaaaag ttaatttata tggaatggaa
    11461 tttttaaacc caattatgac agcggcagga cctggagcaa aagatggtgc tctctgtcaa
    11521 gaggcagcaa aaggtggagc aggagctata tgtacaaaaa ctatatctgt attaccagct
    11581 gatgtaccaa gaccatgtat ggcaaaaact aatagtggtt ttttaaacac agaattatgg
    11641 tcagagcttc caaaagagca atggatagag gaagaataca aaaaggtaaa agaaacaggg
    11701 cttccagtaa tagtaagtat gggatattca gcagagcaaa tagctgaggt tgctccttta
    11761 gtaaagcctt atgcagatgc agttgaatta tctactcatt atgtaggtac agacgttgca
    11821 ccaatagtaa atgctttaaa agctgcaaaa gctgttttag atgtaccagt atttatgaaa
    11881 atgagtcctc atactgacat tcaaactatt gcaaaagctg ttgaagaagc aggtgcagat
    11941 ggacttgtaa tgataaactc atttggacca tgtatggcaa tagatgtaaa tacaggatac
    12001 cctataatgg gaagtaaagc tggatacgga tggttatcag gtgcaccaat aagacctcta
    12061 gcaataagat gtatatatga agcttctcaa gttgtaaata taccaataat cggtgttggt
    12121 ggaatcactt gtggaaaaga cgtggctgaa atgatgatgg ctggagcaag tgctgttcaa
    12181 gtatgtacag aagcaatcct taaagggcct tctatctatg gaaaaattgc taaagaatta
    12241 aatgaatttt tagatgagaa tggatatgct gatgttaatg aaataaaagg attaactcat
    12301 aagaaaatgg cagctagagg gtttagaact cattcagtag ctccagtagt ggatgataac
    12361 aagtgcatca aatgtaagca atgtgtaaca agctgtgtat acgaagcttt agaagttaca
    12421 gataagttga acatagatgc agacaagtgt tttggatgtg gattatgtgt aactagatgt
    12481 ccaaaaggag ctttaagcat ctgctacaat caagtagaag ctatgaatga gtaataatta
    12541 tatagaattt tggtaattaa cttagagtaa taggtattag gataataaaa tatatcagat
    12601 aaacaaacac acattatgca ttaataatac agtctaaagt taataatttt attatattga
    12661 tttaactaaa tattagttta gctgaatatt aatttaatta ggtattggtt taatcttaat
    12721 atagatttac tacgtataag tgaactaact agattacttt aagttaattt tttcatataa
    12781 aataaattga tacattacaa gtaaggtgga gtctatatgg gaattgtttt aaaaggtggg
    12841 aaagtagtaa caagtgaaaa aacttactgt gcagatgtaa gaatagaaga tgaaaaaata
    12901 gtcgatatag gtcttgatat atcaaaagaa aatgatgaaa tagttgatgt tagtgattct
    12961 tatgttatac caggaggtat agatacacac acacactttg acttagatac aggcgttact
    13021 aagacagcag ataactttga gacaggaaca aaagctgcta tagtaggagg aactactact
    13081 atattggatt ttgctacaca agctaaagga tgttctttga aagacggatt agaagaatgg
    13141 catgaaaaag ctaaggataa atcctattca gattatggct ttcatatggc tataacagat
    13201 tggaatgact cggtttgtaa tgaaatggag gatatggtga aagagggagt atcttcattt
    13261 aaactgtata tggcgtataa aggaagcctt caagttgatg atggtgtaat ctttgaagca
    13321 ttgagaaaag cagaagagat aggagggatt attggtttcc attgtgaaaa tggggacata
    13381 atatgtgaac ttgtggataa agcaaagtca gaaaataatt tatctccaaa atatcatcaa
    13441 ttaactagac cagctataat ggaggcagaa gctacatcaa gacttatgaa gatagctgaa
    13501 gttgcaaact caaaagtata tgtagttcac ttaagctgta aagaatcctt agaggaggtt
    13561 ttaaaagcta agcaaagagg tgtggatgta atagttgaaa cttgtcctca ataccttcta
    13621 ttaaatgatg aattatatga tttagatgga tttgaaagtg ctaagtatgt aatgtcacca
    13681 cctttaagaa ataaagaaaa taatgaaata ttatggaatg ctattttaga taatcaaata
    13741 gacactatag gaacagacca ttgttctttt aattataagg gacagaagga acttgggata
    13801 aacgatttca gtaagatacc aaatggttct ccaggtgtag aacataggat gagtctttta
    13861 tacacatatg gagtctgtaa aaataaaata tctatcaaca agatggtaga agtaacatca
    13921 actaatgcag caaagttgtt tggaatgtat cctaataaag gaactataga aataggcagt
    13981 gatgcagata tattagtctt aaatccaaac aaaaaatcca aaatagaagc aaaaaaacaa
    14041 ttacaaaatg tagattatac accttatgaa gggtatgaag tggattgtca aatagaacat
    14101 gtttatttaa gaggaaaaga agtagttaga gatggagaat taatttgtaa tactccaagt
    14161 gggagatata taaataggcg tctaacaaag ggggctagag aatggtagaa tttatattaa
    14221 atggagagaa agttgttgca aaagaaggca aaaaactttt agacttcctt agagaagata
    14281 tgaatataac ttcagttaaa aatggctgtt cagaaggtgc ttgtggaaca tgtatggtta
    14341 taatagatgg taaagctact aaagcatgtg tatttaaaac tgataaatta gctgggaaaa
    14401 acataacaac tatagaggga ttatcagaga gagaaagaga tgtttttgca tattcttttg
    14461 ctagtcaagg agctgtacaa tgtggatttt gtattccagg tatggtaatt agttcaaaag
    14521 cacttataga tgttaataat aatcctactg aagatgatat aaagaaagct ttaaaaggta
    14581 atatatgtag atgtacaggg tatgtaaaaa taattaaagc tgtcgattta gcagcaaaga
    14641 tttttagaga aaatatagat gtgccaaaag taaactgtaa gggtctagta ggtgaaaatc
    14701 ttcctagagt tgatgcagtt ggcaaagcac ttggatattc tgaatatgta gatgatatga
    14761 gaattgaggg tatgatttat ggttcagcag ttagagctaa gtatcctaga gctttagtta
    14821 aaaaaataga tatagaagaa gcaaaagctt atccaggagt tgttgcagtt ctaacagcta
    14881 aagatattcc aggaactata aaggtagggc acttaaagaa ggattgggat actttaatac
    14941 cagaaggtga aataactaga tatttaggag atggaatagt tttagtagca gcagaaacta
    15001 gagaagcgtt agaagaggcc aaaaagctag ttaagataga ttatgaggaa ttaacaccat
    15061 taagtactac agcagatgca cttagagaag atgctccaaa gatacatgag acagggaata
    15121 tacttgttaa ggaacattta gtaagaggaa acgctaaaga aaagatagaa aattcaaaat
    15181 atgttgtaac taaaagatat actacacctg ctacagaaca cgcattttta gagcctgaat
    15241 gtgcagtagc tggtccttat gaagatgatg gagtaataat ctattcaaca gaccaagggg
    15301 tgtttgccac tcaacatgaa tgtgcagata tgttagggtt accatatgag aaggtaagag
    15361 ttataaacaa aatggtagga ggagggtttg gtggaaaaga agacatgagt gttcaacacc
    15421 atgcagcctt acttgcatac catacaaaga gatttgtaaa agtacagtta acaagaaaag
    15481 aaagtattat tgttcatcca aaacgacatg ccacagaaat ggaatttacg acagcatgtg
    15541 atgaaaatgg atacttaacg ggtatgaagg caacgataat atctgacaca ggtgcatatg
    15601 cctctcttgg aggaccagtg ctacaaagac tgtgtactca tgcagcagga ccatacaatt
    15661 atcaagatat tgatataaca ggaacagcag tatatactaa taaccctcct ggaggagcat
    15721 ttagaggatt tggagttaca cagagttgtt ttgcaactga atgtaatcta aatttacttg
    15781 ctgaaatggt tggaatatct ccttgggaga taagatataa aaatgcaata agacctgggc
    15841 aagttttacc aaatggtcaa tttgcagatg aaggaacagc aatggcagag acactagaag
    15901 ctgtaaaaga agagtatgaa aagcatccac gtgcaggtat agcttgtgct atgaaaaata
    15961 gtggggttgg agttggtata cctgatgttg gaagatgtag attggtgata aaagatggca
    16021 aagttcacat aagaacaagt gcttcttgta taggtcaagg gcttggtact gttatgactc
    16081 aagtagtttg tgaaactgct agcctacttc ctgaaaatat agtgcatgaa gttccagata
    16141 ctcatttaac accagactct ggtaatacta cggcttcaag acagacagta tttacgggag
    16201 aagctactaa ggttgcatca aataagttaa aagaagcttt agactctggc aaaactttac
    16261 ttgatttaga gggagaagag ttttatggag aatatgaaag tattacagat aaaatgggtt
    16321 gtgacaaaga aaatccagta agtcatgttg cttatggata tgcaacacaa gttgtaattc
    16381 tggatgaaaa tggaaaacta gaaaaagtag tagcagcaca tgatgttgga agagctataa
    16441 atccaatcag tttagaaggt caaatagaag gtggagtagt tatgggtctt ggatatgctc
    16501 taaccgaaga ttacccagta gttaactctg ttcctacagc taagtttggt acattaggtt
    16561 tatttaaatc tacagatata ccagaaatac aagctttaat agttgaaaag aacaaagatt
    16621 tattggctta tggggcaaaa ggtgtaggag aaatttgtac agttcctact gcaccagcag
    16681 tacaaaatgc ctattatgta tttgataaag aatttagaac tgagctacca ctacaaaata
    16741 ctccatatag taaaaaaaag taacaaaaaa aagaaatatt aaataaaaaa atagatgaaa
    16801 ttgtaaataa aaaaaaccct tttgcataag gtatatttta aatgatagaa tatacatata
    16861 caggagggtt tttcttttat aaaataagtt taataaaatt gagaggtgag cagaatgaat
    16921 tttaataata gtctagaatt aactcagtct cagaagctaa taatgactac acaacttaag
    16981 caatcattat caatattaaa tatgagtaag ttagagttag aagaagagat aaaaagagaa
    17041 gcagagaata acccattagt tgaagtcgag aaaagtgggg agataaattg ggaagagtat
    17101 ataaaagaca tggataggtc aagacgttta gatagaacag agataagcta taatcctgat
    17161 aatgaagtta atctagaaaa tttagttaaa tattcgtcaa atttatatga agatttaaaa
    17221 tttcagataa gcctgtataa acttacagac aaagaattgg aagtctgtga gtatataatt
    17281 gatagtttag atgaggatgg atatttaaga actgaggaaa aagtaattgt tgatacctta
    17341 aagatagatg aagaactttt tgaaaaatgt ttaattagtg ttcagcagtt ggaacctagt
    17401 ggagtagggg caagaagtct ttcagaatgt ctgatgatac aaatggctag tttaggaata
    17461 tataatgaaa ttttagagga aatcgttaca aaagacttaa atttaattgc aaataataaa
    17521 tataaagaga taagtaaaaa gtacaatatg tcattacaaa aatgtgttga actaataaac
    17581 ataattaaga cattagaccc caaaccagga agaacttgtt cagtagagaa aagtgtatac
    17641 attcaacctg atgtaacagt agaaaagatt gatgatgaat ttattgtata tttaaatgag
    17701 aaagattctt accaaataag aatcaatagt tattataaag acatattaaa aaattcacag
    17761 tctgatgaaa gtgcaaaaga atttataaaa gaaagactaa attcagcaac aggacttatg
    17821 aaaaatatag agagtaggaa aactacagta cttaagattg cagaagagat agtaaaagct
    17881 caagatgaat ttttgagaaa agggacaaaa tatataaaac ctttgaaaat gaaagacata
    17941 gcagaaaaat tagaatttca tgaatctact ataagtagag gtgtaaatgg taaatatatg
    18001 ctaacacctt ttggcgtata tgaatttagg tactttttca gtagcgccat tgagactgag
    18061 aataatgaaa tggcatctag tacaagcata aaaaaaatta taaaggaaac aataaaagac
    18121 gaaaataaaa agaaaccttt aagtgatgac catatatcta agttattaaa agaaaaagga
    18181 atcaatgttg caagaaggac agttgccaaa tatcgtgaag aattaggaat cccatcttca
    18241 agtaagagaa aagaatacta ataagtaaaa gacaaatatc agtatggtaa ggggaaaagg
    18301 ctaattaaga gatgaatgta gattgggcta aaaattttat aaagtaaaat aatagaatat
    18361 ggaaaagtat taaaatgcta taaggtatta aaaaactggg gaacattaaa acattaagat
    18421 agcaaagaac ataaaaaact aaagaagatt aaaacattaa agaacattag gctttgagga
    18481 atattagaac tttaaagaat attaaaattt ataaaatata aaaatactaa aaagaattga
    18541 aaaactgggg aacattaaaa tattgtaaaa tgctaaaaaa ctagaaaaca taattaaata
    18601 agtgcttata aagaatgatt aaattaaata agtgtttgta aagaataatt agatattaga
    18661 aaaagaagaa tacctagata aataaactat tattaaattt aataatcaca aaaggaaaac
    18721 acgtagggta taaataaaaa tcttaactag ataaaagtaa acttagttgt agataaaaat
    18781 aactagaaat aaaagtaact agaaataaaa ttatttatag taataaatat tagaatgaaa
    18841 atataaaaaa atatatataa aaagccttat ataaaaagaa aaaatactgt gatttgaaaa
    18901 tggtggagaa atattttata aaataaaaaa ataaaaaagt ttttgaaaaa ctattgaaat
    18961 ctatacgcaa atcctataaa ataaaaatat cttgtgggac ttaaataaac actagggaca
    19021 aaaaaagtcc cgaaaggaaa gcaataagga gtatgttatg aagaacttat tagaaattca
    19081 aaagaaattg attccacagg caattgaact tatggaaaga agatactcta ttttaagaca
    19141 aatatcttta agtgaaccaa taggtaggag aacattatca aatgtattag agattagtga
    19201 aagaataatc aggtcagaaa ctgagttttt aaaagaacaa ggacttatag acgtagctgt
    19261 atctgggatg actatcacta aagaaggttc tgatttgtta gataaactta aggacatcat
    19321 gagtgacatc atgggattgt ctaagttaca agatagagtc agagaaaagc ttggaataaa
    19381 aagggtaata ctagttcctg gaagctgtga tgaaaatgga agtttactaa aagatgtaac
    19441 tagatatgga tgcgagtatt tcttaggcat tttaaaagat ggagatatcg tttcaataac
    19501 aggaggaagc actatgttgg agttttcaaa cgttattaaa actgataaga gatatccaaa
    19561 ctctacaata gttccagcta gaggaagtat gggaactgat gtagagatgc aatctaacaa
    19621 tatagttgct tcaataagta agaaattaca ttctaattgc aaacttttac atataccaga
    19681 tgagttggat gaaagtgcaa tgaaaactct tagccaagtg ccagagatta agacaacttt
    19741 ggagtatatt aaaagaacag acattttagt ttttagtata ggtagagcag atgtcatggc
    19801 aaagagaaga aaacttccag aagacaaggt tgatgagata ttatctaaaa atgcagttgg
    19861 agaagcattt ggatattatt tcaacaaaga tggagaaata gtttacaaac tgaacactgt
    19921 tggaattgat ttagaaacag ttaaacaagt aaaagaaact attgcaatct ttgcagggac
    19981 tgaaaaagta gaagccttat tagctatatc aaatatgcat aaaaacatgg ttcttgtaac
    20041 agatgaagaa agtgcttata agatattgtc attattataa aagtcaagac ctagcacaga
    20101 gctagaaaac atacatttac tttataggag gtagcgaaaa tggttaaagt agctataaat
    20161 ggatttggaa gaataggaag attagcatta agaaaaatga tggagcaaca agataagttc
    20221 gaagttgttg caataaacga cttaacagac gctaaaatgt tagctcattt atttaaatat
    20281 gatacagctc aaggaagatt taacggagaa atagaagtta aagaaggtgc ttttgtagta
    20341 aacggaaaag aaataaaagt aacagcagaa agaaatcctg ctgacctacc atgggctgaa
    20401 ttaggagtag atatagtatt agaatgtaca ggattcttta catctaaaga taaagctgaa
    20461 gctcatatac aagcaggggc taagaaagtt gttatatctg caccagctac tggagattta
    20521 aaaacaatag tattcaacac aaacagtgac atattagatg gttctgaaac agttatatca
    20581 ggagcttctt gtactactaa ctgtttagct ccaatggcta aagtattaaa tgataaatac
    20641 ggtatagaaa aaggattaat gactactata catgcataca ctaatgacca aaatacttta
    20701 gatggtcctc atccaaaagg tgatttaaga agagctagag ctgctgctgg aaacatagtt
    20761 ccaaacacta caggagctgc aaaagcaata ggcttagtaa taccttcatt aaaaggaaaa
    20821 ttagatgggg cagcacaaag agtaccagtt gtaactggtt caataactga attagtttgt
    20881 acattaggaa aaaatgttac agttgaagaa ataaatgcag ctatgaaaga agcttcaaac
    20941 gaatctttcg gatatactga agaaatgtta gtatcttctg acataatcgg aataagctac
    21001 ggttcattat ttgacgctac tcaaacaaaa gttatggaag tagatggaaa acaattagtt
    21061 aaagttgttt cttggtatga taatgaaatg tcttatactt ctcaattaat aagaacttta
    21121 ggatactttg cacaattagc aaaatagtca gttttgagaa aatagaaaag tagattgaca
    21181 aagtccgagt ttgtagttta atactacgga ctcaggactt tttttaagaa aaaatatata
    21241 aaatacacct aaataatgtt acatactata tataaaaata tgataatatg atatatttaa
    21301 tagggtaaaa gtatgtacta tattatactt ggcaataaaa aattacttta ttaaaaggag
    21361 agatatttat gtcaatgctt aacaaaaaaa ctatagaaga tattgatgtg tgcggaaaaa
    21421 aagtattagt aagatgtgac tttaatgtgc cattacaaga tggtgtaatt actgatgaaa
    21481 atagattgaa cggagcttta ccaactattc aatatttaat ttctaaaggg gcaaaagtta
    21541 tattatgctc tcacttagga aagccaaaag gagaagctaa accagaatta tctttagcac
    21601 cagtagcaaa gagattatct gagatgttag gaaaagaagt tgtatttgca gcagacgata
    21661 atgttgtagg agaaaatgca aagaaagcaa ctgaaaaaat ggaaaacgga gatgtagtat
    21721 tattagaaaa tactagatat agaaaagaag aaactaaaaa cgaagagaat ttctctaaag
    21781 aactagcatc acttgctgaa atatttgtaa atgatgcatt tggaactgct catagagcac
    21841 attgctcaac agttggtgca ggcgagtttt tacaagagag agtttgtgga tacttaattc
    21901 aaaaagaatt aaaattctta ggagaagctg ttgcaaaccc tgtaagacca tttacagcta
    21961 tacttggagg agctaaagtt tctgataaat tagctgttat aaacgaatta ttagaaaaag
    22021 ttgataatct aataataggt ggaggaatgg cttatacatt cttaaaagct cagggttatg
    22081 aagtaggaac ttctttactt gaaatagata aagttgaata tgctaaagaa atgatggaaa
    22141 aagctaataa taagggtgtt aacttattat taccagtaga cgttgttatg gcagaccact
    22201 ttgcaccaga tgctactcca atagtaacag aagatgctaa tgtaaaagaa gattacatgg
    22261 gactagatat gggacctaag actatagcaa actttgtaaa aactataaaa gaatcaaaaa
    22321 ctgtagtatg gaatgggcca atgggagtat ttgaatttga aaactttgca aatggtacat
    22381 tatcagtagc tagagctatg gcagagttga ctgatgcaac tactgtaatt ggcggtggag
    22441 atagtgcagc agcagttaat caattaggat ttggtgacaa gatgactcac gtatcaacag
    22501 gtggaggagc atcactagaa tttttagaag gtaaagaatt accaggtatt gcagcattag
    22561 ataataaata aactaatata tagaggagat tgtaaaatga gaaaacctat aattgcagga
    22621 aactggaaaa tgcataaaac aataaaagaa gctttagaat ttgtaaatga agttaaagat
    22681 aaagtaaatt ctgataaagt agaagctgtt atatgtgcac catttacttt attaaaggat
    22741 ttaaaagaag ctactaaggg tacaaatata aagataggtg ctcaaaatat gcactttgaa
    22801 gaaaaaggag catttacagg agaagtttca cctcttatgt taaaagaaat agatatggac
    22861 tatgttgtaa taggacattc agaaagaaga caatacttca atgaaactga tgaaactgta
    22921 aacaagaaag ttttaaaagc attagaagta ggaatagacc caatattatg tgttggagaa
    22981 actttagaac aaagagaagc tggaaaaact aaagatgttt gtaaagttca agttgaaaaa
    23041 gctttagaaa atgttcttaa agatgattta tcaaaagtag ttgttgcata tgagccaatc
    23101 tgggctattg gaactggtaa aactgcaact gctgaagatg ctaatgacgt tatatcttat
    23161 ataagagaag ttataaaagg attatatgga gaattagcta atgaagttag aatacaatac
    23221 ggtggaagtg ttaaaccttc aaatgtagcc gaaataatgg gtcaaagtga tatagatgga
    23281 gctttagtag gtggagctag tttagctagt aatgattacc tagaccttgt taatttctaa
    23341 ggagtgaaat gatgaaaaaa ccagttgctt taattattat ggacgggttt ggatacaata
    23401 aggatgttaa gggaaatgca attgctgagt ccaaaactcc taatttagat agaataaaaa
    23461 aagagtatcc aaacacattg ataaatgcta gtggtcttga tgttggtcta cctgatggtc
    23521 aaatgggaaa ttcagaagtt ggacacacta acataggtgc aggtagaata gtatatcaag
    23581 atttaactag aataacaaaa tctataaaag atggtgactt ttttgctaat aaagttttat
    23641 gtgaagcaat ggataatgca aaagaaaatt ctcttcatgt aatgggactt ttgtctgatg
    23701 gaggagtaca ttcacatata gaccatctta aggctataat caaaatggca aaagataaag
    23761 gcgtacaaaa ggtgtatgtt cacgcattta ctgatggtag agatactgac cctcaaagtg
    23821 cattagaata tgccaaagaa gtacaagcta gtatggatga aattggagta ggtgaatttg
    23881 ctacagtttc tggtagatac tacgctatgg atagagataa gagatgggaa agagtagaac
    23941 ttgcatacaa tgccatggtt agaggtattg gtgaaaaagc aaattctatt gaggaagcta
    24001 ttcaaaactc ttatgatgat ggaaaaaatg atgagtttat aatgccaaca gttattatga
    24061 aagatgataa accagttgga agtataaagg aaaatgattc aattatattc ttcaacttta
    24121 gaccagatag agctagacaa ataactagag ctttagtttg tgaagagttt gatggattta
    24181 aaagagaaga tattaaaaac ttctttgtat gcttaacaga gtacgatata actatagaga
    24241 atgtgcatat agcatttgga cctcaatctt tagccaacac tttaggtgag tatctagcta
    24301 aaaatggaaa aactcaactt agagctgctg aaactgaaaa gtatgcacat gtaactttct
    24361 tcttcaatgg tggagtagaa gagccaaata aaggtgaaga aagactattg ataccttctc
    24421 caaaggttgc aacttatgat ttaaaaccag aaatgtcagc atatgaattg acagataaag
    24481 ctttagataa attaggagaa gataagtttg actttatagt attaaacttt gcaaatcctg
    24541 atatggtagg tcacacagga agtatagaag cggctataaa ggctgttgaa actgtagata
    24601 cttgtgtagg aaaattgata gataaaatag tagagttagg tggaagtgca ataataactg
    24661 ctgaccacgg aaatgctgaa tatatgttag acccggaaac aggaaaaact gtaacggctc
    24721 actctattaa tccagtacca tttattgtag ttggtcaaga atatgaaagt gcaaaattat
    24781 tggatggagg aagattatct gatatagcac cgactgtttt agatatgatg aagttagaaa
    24841 agccagaaga aatgactggt cattcactta tttcaaaata gtcgattctg ataagtagag
    24901 aataatatat aaactacaat ataaatttaa aaataatgca gtttaggttt attttgtaaa
    24961 gtttagactt tatgacataa gatttagatt ttgtaagttc tggatattgt aaggtgtaga
    25021 tgaaggtata ttttataaaa tttacaagat agacaattta taaaataaaa aattataata
    25081 tagataataa tgtaaataat gatataaata ataacaagat agacaataac aatataaata
    25141 ataataatta atatatatta tataagcaaa aattttagaa tcattagttg tacattttat
    25201 gttt
//
LOCUS       DAEPLF010000033        24810 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEPLF010000033 DAEPLF010000000
VERSION     DAEPLF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24810
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.033"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            120..749
                     /locus_tag="KR314_001232"
     CDS             120..749
                     /locus_tag="KR314_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327626.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            750..1532
                     /locus_tag="KR314_001233"
     CDS             750..1532
                     /locus_tag="KR314_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0327627.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAINTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1525..2403
                     /locus_tag="KR314_001234"
     CDS             1525..2403
                     /locus_tag="KR314_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327628.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIIGKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIKIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSEYYI
                     KLKKDILMTMKSNKDI"
     gene            2436..3395
                     /locus_tag="KR314_001235"
     CDS             2436..3395
                     /locus_tag="KR314_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0327629.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDVALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3709..4401
                     /locus_tag="KR314_001236"
     CDS             3709..4401
                     /locus_tag="KR314_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0327630.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4411..5415
                     /locus_tag="KR314_001237"
     CDS             4411..5415
                     /locus_tag="KR314_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0327631.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGIHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKM
                     "
     gene            5663..6514
                     /locus_tag="KR314_001238"
     CDS             5663..6514
                     /locus_tag="KR314_001238"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBH0327632.1"
                     /translation="MSLDVIFILKSVIIAIVEGFTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6618..7364
                     /locus_tag="KR314_001239"
     CDS             6618..7364
                     /locus_tag="KR314_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327633.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7364..9280
                     /locus_tag="KR314_001240"
     CDS             7364..9280
                     /locus_tag="KR314_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0327634.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTYFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLIYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            9559..10476
                     /locus_tag="KR314_001241"
     CDS             9559..10476
                     /locus_tag="KR314_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBH0327635.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYKSEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSIIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILSEIEKMKKIY"
     gene            10554..11291
                     /gene="cas6"
                     /locus_tag="KR314_001242"
     CDS             10554..11291
                     /gene="cas6"
                     /locus_tag="KR314_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBH0327636.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KAYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKIVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            11297..13174
                     /gene="cas8a1"
                     /locus_tag="KR314_001243"
     CDS             11297..13174
                     /gene="cas8a1"
                     /locus_tag="KR314_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBH0327637.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQIMFNDYLRQIIYFGELADLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKEYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTSINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHLTNNILRNKVKNDMESNNKSNISGIDCFRVVQLRALINSYKKG
                     VYKMDSKNLEKNDEKLRIIYYLGCDIHDYFVNKNSKNKIDGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQIPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            13176..14090
                     /gene="cas7i"
                     /locus_tag="KR314_001244"
     CDS             13176..14090
                     /gene="cas7i"
                     /locus_tag="KR314_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBH0327638.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            14092..14793
                     /gene="cas5b"
                     /locus_tag="KR314_001245"
     CDS             14092..14793
                     /gene="cas5b"
                     /locus_tag="KR314_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBH0327639.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            14804..17125
                     /locus_tag="KR314_001246"
     CDS             14804..17125
                     /locus_tag="KR314_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBH0327640.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQREFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIIGVGEANDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLADLTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIHENKHTKQIFGKGVLISNEIDQFI"
     gene            17140..17691
                     /gene="cas4"
                     /locus_tag="KR314_001247"
     CDS             17140..17691
                     /gene="cas4"
                     /locus_tag="KR314_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBH0327641.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEMLIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKEKIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            17694..18674
                     /gene="cas1b"
                     /locus_tag="KR314_001248"
     CDS             17694..18674
                     /gene="cas1b"
                     /locus_tag="KR314_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBH0327642.1"
                     /translation="MYKDYYIISNGNIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNTLMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            18677..18943
                     /gene="cas2"
                     /locus_tag="KR314_001249"
     CDS             18677..18943
                     /gene="cas2"
                     /locus_tag="KR314_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBH0327643.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITDGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   19127..20677
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19127..19155
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            20867..21295
                     /locus_tag="KR314_001250"
     CDS             20867..21295
                     /locus_tag="KR314_001250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBH0327644.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIEPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(21751..22530)
                     /locus_tag="KR314_001251"
     CDS             complement(21751..22530)
                     /locus_tag="KR314_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327645.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMSSEVSSIRCCNCDELLEDITLNKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
     gene            22854..23399
                     /locus_tag="KR314_001252"
     CDS             22854..23399
                     /locus_tag="KR314_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBH0327646.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            23431..24567
                     /locus_tag="KR314_001253"
     CDS             23431..24567
                     /locus_tag="KR314_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBH0327647.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CNLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIERADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     PILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKTKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIERYNIFGGALAYGH
                     PYGASGAIITLHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
BASE COUNT        10020 a         2291 c         4178 g         8321 t
ORIGIN      
        1 ttaaaaaatg tagataaaaa ttgttttatt aaggaaaaat taatatattg acaaaattta
       61 agtgctcatt taaactatta attgataata atattcaata ttaaataata ggaggcgtta
      121 tgaataaata tcttctaata aatccagtgg cagaaagaat gtatggagaa aagtttcacc
      181 ttattaaagc aaatttgata aaaaaggggt acataatagc agaatgtgaa ccacagttag
      241 aatatgtaaa aaagcagtac aaagagtata caaaaaagac tgaaaataca atgttggatt
      301 gtagatgtcc agaaagtata agtcttttaa aaagaaataa tttgataaat aattttgaag
      361 tacctatgat agaaccaata ttagtaagga cttgtagggt tttatacgat aaatatataa
      421 aaagtaaaga tgatatgttg gtagtaacct gtccatgtac acaattaaga gactttgcaa
      481 gtgaaaagtt taaggataag ggtaatgcta tgttttatac atggaaagag tttgctgaac
      541 aagaaggtgt aaaatcactt ggaaaaatag atgaatcacc aattccatta ggatatttca
      601 aagatacttt tgaaaaagtt cttgaagtat caagtgaaga cgaaatatta agtgaaatac
      661 aaaaaataag aaatggcttt gaagataagt atgacatagt agaaatgttt tattgtaaag
      721 atggttgtaa taatggagat ggtttgtaaa tggtcaataa atttaataat aagtttttaa
      781 ggctagtaaa gagtgttctg atatttttta taattctttt attatggaaa attacaaatt
      841 atcttggtat ttggagtgat tatatattac catcacccga aaaggtatat agtacatttt
      901 taaatatgat aagtgatggt tcaatattta ttaatgtata tgcaagtatg aaaagagttt
      961 tgataggttt tgcaataaat actgctatag gtgtgccttt agggatattc tttggaattt
     1021 atagtggagt atacgaatac tttaaatctc taataaattt tttaaggaat acacctcccc
     1081 ttgctttaat accaatgctt atattatggt ttggtatagg agaggaatca aaaattataa
     1141 ttatagtttt agcatccttt ttcccaatct ttacaagcac attgaaaggg attaaaaatt
     1201 gtgactcaaa actgattgaa gtgggtagag tatttgagtt ttcaaagctt cagataatat
     1261 ttaaaataat aataccaaat gccattcttg atattgctgt tgggttaaag ttagctcttg
     1321 gatatagctt tagagcaatt attggggcag aacttgtagc agcttcatct ggtcttggat
     1381 acttgatatc tgatggaaaa gaaatgtcta gaacagatgt tgttattgta ggtataatcg
     1441 taattggact tttaggaata attacagatt acatattttc gattattgtg aagaaggtaa
     1501 gtaagggaaa gatggtggaa gcatatgaat aatttagtat gtgactctgg atatataatt
     1561 agaaatttat ataaaagtta taaagttgac aatgaagaac atgtagttct tagtgatatc
     1621 tctttaaaca tagatagaaa ccatataaca gtaattttag gtgaaagtgg ttgtggaaag
     1681 actaccttgc ttagagcaat tgcatcactt gaaggtataa atagtggtga gattaggttt
     1741 attaatgata ataaagagta tagacctaat gttggttttg tttttcagga gagcagatta
     1801 atgccttggt taaacgtaag tgaaaatata gcttttcata atcaaagaaa aaatactatt
     1861 ttaaacaagg tgcttagaaa atttaaacac aataaaagaa ttattggtaa aattataaat
     1921 gataatttta gaagcttaga aacaaatgat aatttgaaca aatcaaataa atataatagg
     1981 aaaagtaata ttgatgtaga aaaatatctt aagatgatga acttgcagaa atttaaaaac
     2041 tcttatccaa atgaattgtc aggaggtatg gcacagagag taagcatagc tagagctctt
     2101 tctttcaatc cagatatgtt gcttatggac gagccttttt cggcattaga ttattttaca
     2161 aggattgata tgcaaaatga agttataaaa atacataagt caactaataa aggcgtaata
     2221 tttgtaactc atgatattga tgaagcgtta aagataggca aaaaaatagt tgttttcaca
     2281 gacgaaagaa aaataaaaga atttaatata gaagatggtt ataatagaga tttaacttca
     2341 gagtactata ttaaattaaa aaaagatatt ttaatgacaa tgaaatcaaa taaagacatt
     2401 taaagaaatt aataatattt taaggagtag agtaaatgaa attaaaaaag ctattgatag
     2461 gtataacgat attaacactt gcaacagcat ctttagctgg atgtgctaaa aaaaataatg
     2521 gagaaaaact ttcagaaata aatttaactt atgtaaaatc accattaaat gttccttcta
     2581 taattcagaa acaggatgat ttatttggaa aggaatttaa aaaggataat ataaaagtta
     2641 actttcacga aattactaca ggtccagagc aaacacaggc tttagctgct ggagaaatag
     2701 acttcttaca tgcactaggt ggtacgtcag ctttaatagc tgcatctaat ggagtagagt
     2761 taaagatatt aaacacttat agcaggtcac caaaaggatt tatgattttg actaataata
     2821 attcaataaa atcagcagca gatttagtag gtaagaaggt ggcaggtcca aaagggacta
     2881 tattacatca ggtattgatt tcagcacttg ataaagaagg attaagtatg gatgatgtgg
     2941 aatttgttaa tatgggtatt ccagaagcat cagcagctct ttctgatggt agcgtagatg
     3001 ttgcattgat tgctggtcca gcagctctta aagctatgaa gtcaggtagt aaacttgtag
     3061 caaatggtga aggtttagta gatggtataa tagtaactgc tgttagcaca gactttgcag
     3121 agaaacaccc agaaatagtt gaaagattta tgaaggttga aaaggaaact ttagaatatg
     3181 taaataataa ttttgatgaa gctatggaaa aagtagctaa agaagttgat ttaagtcttg
     3241 aagaaactaa agaattatat gcttggtatg attttagttt agatataaca gataaagata
     3301 tatcttcgct tgaagataca caagacttct taataaaaaa taagttacaa gagaaaaaag
     3361 tgaatataaa agaattaatc tataatgcta agtagaaact atgctaaaat aatactaagt
     3421 aagaaatata ataaaagttt aagttacctt attaaggtaa ctttttctat tttgaattag
     3481 taatttcaat ttattttaga atgattagtt gataataatg attaattaat cataaaaaga
     3541 tactttttta tagagtttta ttaagatgtc taataatatg gttatactta tgaagtcagt
     3601 aattgtgaag ttaataatat gattaaattt atatattgta aaaagattat aatatattaa
     3661 aattggactg ttatatgatg agtaaatagg tatattagga ggaattatat gagttataaa
     3721 gtatatattg tggaagatga tatatcaata agtacattac ttaaagatta tattgagaaa
     3781 tatggatttg aagtgattgt tgaagagaac tttgatgata taatgattac atttgaaagt
     3841 tttaagccac acttagttct attagatgta aatttaccta aatttgatgg tttctactgg
     3901 tgtagatgta ttagacaaca gtcgaattta cctataattt ttatatcagc tagagatggc
     3961 aatgtagacc aaattagagc tttagaaagt ggagcagatg attatataac aaaacctttt
     4021 tactatgatg tagtaatggc aaaaataaag agtcatataa gaagggtata tggtgaatat
     4081 tcacctaaaa tagacgaaag aatagtggag ctagatggat taaagtttta tccagaaagg
     4141 ttagaactag aattaaatgg tgaaaatcta attataacta aaaaagaagg tatactgatt
     4201 gaatgtctta tcaagagata cccaaaagta gtaagtagag attatctttt agaaaagata
     4261 tgggatgata ttgagtttgt agaagaaaat actttaaatg taaatgttag caggataagg
     4321 aaaagatttg aggaattagg tatagataat gctgttcaga ctgtccgagg gttaggatat
     4381 aaattaaata agaattggta aggagaaagt atgaagttat ttttaaaaga ttataaaggt
     4441 tatattttta tatattatat aggcatagtt atgacaatta tttactgtaa tcttatgaag
     4501 tttattagtt ttggagagag tttatatatt cttttattta gttcttttat attgttatgt
     4561 tttttaatat ataagtatta taaaaataga gaagtatata atctgtttga aaaaggaata
     4621 cattctttgg aggagtcatt tttgtattta ggaagctcat tcttggggag aaatatatca
     4681 gttattttaa aacaacaaca taaattgtat caatcaatga tacaagagca taaaaagata
     4741 catagtgaac atttaacttt catcaaccaa tggattcatc agatgaaaac accaatatca
     4801 gttataagcc ttcagcttca agattatgag ggagaagaag taactgaaaa tataagaaaa
     4861 gaaatagata gattaaataa agggttagat atggctatgc attttgcaag ggcagataat
     4921 tttcagaaag actttatagt tgaaaaagcc aaattaaatg aaattgtatc tagtattgta
     4981 aatgaagaaa aaaggttatt tataaaaagt ggaatgattc ctaagattca agtagacaaa
     5041 tttacttatg tttatacaga tgtaaaatgg atgaaattta taataggtca attaattaca
     5101 aatggagtga aatattctaa agattatagt aactacctaa ctataaaatc tgaagaaaat
     5161 aataaatatg taattttaag tataatagat gaaggaattg gaattgtttc taaagattta
     5221 aaaagggtat ttgacccatt ttttacagga gaaaatggtc gtaagtttgg agaatctaca
     5281 ggtatgggtc tttatctagt aaaaaaggta tgtgatgatt taggtcataa agtaagtata
     5341 aaatcagaag taggaaaagg tacagaagtt tcaatctact ttaataaaga tgtaaatagt
     5401 ttgataaaga tgtaaattat ctgttacaat agggttttta gttaattgta cttttttatt
     5461 atacaattta ttaaatgaaa tattcatagt taaataatca aaatttaaga gagtttattt
     5521 gcaaacctta catagatgta aggtttgtgt aatgtttgca gatagttaaa ttttctaatc
     5581 taaattacaa ttaatataag aaataacatg agagaaataa cataaaaaaa gaaattttgt
     5641 aaaaaaggaa ggatgtttat tgatgagttt agatgttatt tttatattga aatcagtcat
     5701 aatagctata gtagaggggt ttacagaatt tattccagtt tcatctacag gtcatatgat
     5761 tttggttggt aatttaatag attttaaggg gcaatttgct gaaatgtttg aggttgtaat
     5821 acaacttggg gctatattag cagtagtagt actttattgg aaaaagataa aagatagcgt
     5881 tatagaattt tttaaattta tatttactgg cggaaaagaa ggaaagattg gttttagatt
     5941 tggaatgaat gtaattatag gttgtatacc atttgcgatt ataggagttc tattttatga
     6001 taatataaaa tcattgttta atttacaatc tgtaattata ggatttatag tgggtgggat
     6061 tttattatta gtagtagaaa ctttgtttag aaaaaagaat cattcaacag ataatataga
     6121 taaaattact ccaatacaag ctttaaaggt tgggacatta caagttttgt ctgcatggcc
     6181 aggtatgtca aggagtgcat ctactattat gggtggatgg atagcaggac ttaattctcc
     6241 tacagcagct gagttttctt tctttttagc agtaccagct atggtggctt cttcaggaaa
     6301 agatttattt gaatttgatt attcaataat gacaccaaca ttgtggattg ctttggtagt
     6361 tgggtttata gtagctttta ttgtatcaat aatagttatg gaaaaattcg tgaatttttt
     6421 aaagaaaaaa ccaatgagag tgtttgctgt atacagaata atcatgggtg tagtacttgc
     6481 agtattagct tttactaaca taattagtgt atagaaaaaa tagatttatt atctataaaa
     6541 attaaactaa gataaattat aattaacaat tatatatatg ttatttcaaa aattaaatta
     6601 ggaaggatag ttaaataatg aaaattttaa ctgtaaataa tttaagtaaa gtatatggta
     6661 aaaaaataat tttcaacgca ttaaatgaca taaattttag tatagaagat ggagaatttg
     6721 taggaattat gggtccatct ggtagtggaa aaactactct tttaaatatg atttctacta
     6781 ttgacaagcc aactacagga actatggaat taaagggaaa gaatccttta ttgttaagag
     6841 gtgaggaact tgcattattt agaagaagag agttaggttt tgtatttcag gattttaatt
     6901 tattggatac attaactata ggagaaaata tagtattgcc attgacatta gataaggtca
     6961 gtgtaaaaga gcaagatgag agattgaatg aagtatctac aatattaggt ataaaagatt
     7021 tattaggaaa aagaactttt gaagtatcag gtggtcaagc tcagaggact gctattgcta
     7081 gagcattgat taacaatcca tcaatacttt tagcagatga accaacaggt aatttagatt
     7141 caaagtcatc aaaggttgta atggagctat ttcaaaagat aaataaagaa aataaagtta
     7201 ctactatgat ggtaactcat gacccattag cagcgagcta ctgtagtaga atcttattca
     7261 taaaagatgg gtctatatat aatgaaatat ataagggtag tagtagagag cagttttatc
     7321 aagagataat ggatgtactt acattattgg gaggcgataa ctaatgaata taaaacagtt
     7381 tgctattaac aatatttcaa gaaatattaa agcgtattta ggatacttag caagtattgt
     7441 tatatcatct tcactgctat tttcttttat tatgtttaca agtcatccag atttagatgt
     7501 atctcaattt cctgattatt taaaagaagg tctcaagatg agtaagataa ttgcatatct
     7561 attcttattc ttctttgtgt tttattcagt tagtgtgttt cttaaaagta gatataaaga
     7621 atttggaata ctttatatta caggtatatc aaaacgccaa gtaatgaaat taatctttat
     7681 agaaaatatg ataataaata tagtatcttc tgtaattgga ataataatag gcttaatctt
     7741 tgcaaagata tttttagtgg ttatgtctac ttttttagaa ttatcgccat taaaattcta
     7801 tataccattt aatgcgatat tgtctacttt aatctatttt atgattttag ctgtattaac
     7861 atcaatattt acttatttta tagttaaaga aaatcaggtg ttgaagttac ttaaaggtac
     7921 aaaaactcca aaaccagagc ctaaaacatc actaatgctt gctatattaa gtatttgttt
     7981 gtttataata gcgtattata atgctattac ctcaaccgac caatatacaa catatctgcg
     8041 cttagttcca gttacatccc ttactatagt agcaacttac ttgttctttt ctcaatttag
     8101 tgtatttttt atgaagaagc taaaactaaa caagagattt tatagaaaaa atacaaatat
     8161 gctttggatt tcgaacttga tttataaggt aaaggataat gctagaatat ttttcttgat
     8221 aactataact tctgcagtag catttactgc tataggtaca gtttattcct tttggaaaga
     8281 tgtagagcgt caaattaatt taatatatcc taacacaata tattattcaa caatgacatt
     8341 gcataatgac cctaaaaagc ctgatagcaa agaaaaagac aaggaaagaa tgatgtttat
     8401 agaaaataat ctaaaaaaag agaatgtaga ctatacaagg actaatggtg aatttaaaac
     8461 tgtatttccc aagaaaagtg attttaatgt cagaattatg aaagaatcta agtacttaga
     8521 aattacagaa actatagggg ttaagcctat aagttttgaa gataatgaat gcatatcatt
     8581 attatcatca agtttaccag gaaataataa cgtcaaagaa aatattattg tagggaatag
     8641 aggtttaaag gtggcaaaac aagtggaaga atgtgttatg ccagcatatt acaaaaatat
     8701 gtatgtggta aaggataatt tttatgatag tatagaatca aaatttatag tggatagatt
     8761 tactgcattt gaagtagagg attcaagtaa aactatagag atatgcagag aatttgaaaa
     8821 taaatttaat aatgagtcag ggatgcaacc atatttgttt ttctctaaag aattaatgat
     8881 aggaactggg aaattaatat attctacatt tatattttta gcaatattta taggtttaat
     8941 tttctttgtt actacaagta gttttttata taataaattt tatatggatt gccaagttga
     9001 taagaaaaag tatgagcaat taaataaatt ggggatgaca tataatgaga ttaaaaaagc
     9061 ttcaacaatt gagattggaa tagtattttt gtttccttat gtagtggctg ttatacattc
     9121 aatatttgcc cttactgcac tgaaaaattc ttttgatata gaagtagcat catcagctgt
     9181 tttggtaatg ggaagcttgt tcttggttca aatagtttat tttttaattg taagaaataa
     9241 ttatttaaaa gagataaggt taaatttagt caatttttag ttactattat taaatgataa
     9301 taaaaagggt ttaaaattaa ttaatattac tggaattata gatgtttatt ttttcagtaa
     9361 tattttttat tgtattaact attaaaattt tgtacaaata taaaatataa tatggtaaat
     9421 gtcaaaatta taaaaaaatt ctacttatat aaaatataaa gaggttatat gtacatatgt
     9481 gtagaaataa ataataaaaa tatcaattaa tgttattagg gggggttact cattagtaaa
     9541 ttagctaata cacttagaat gatgatatta attcaaagca gaggcaaaat gaaatgcaaa
     9601 gaattatcag aagagttgga agtaagtgaa aggcaaataa aaagttacaa gatgtattta
     9661 gagcaagctg gtatatttat aaattcaaca cctgggattt atggtggtta tgaaatagat
     9721 aaatgtaata gcatatcatt aattaaatta ttagatagtg aagtttcaat tctagatatg
     9781 ataaattcac agttagagta taataatgat atttacaaaa atgaatttaa taatattgta
     9841 gaaaaaataa aagcagtatt aaatacagga gaaaaaagcg atacatatat ggattatttt
     9901 acagttcaag cacaacgtaa ttgtgattat aagtctgaaa aaaataagtg taatgaaata
     9961 attagagcat ataccacaaa acacaaattt tggatagaat attattcatt aaattcagga
    10021 aatagtgaaa gaattgttca tccatatggt ttatttaatt ataaatcaga tacttatatg
    10081 gtagcttttt gtgaaaaaag atttaagttt atagacttta aattatgtag aattaaagat
    10141 tataaggttt tagaagaaaa atataatgta gataaaagtt ttagttggga tgaatatagt
    10201 aaaaatagta taggaattta taaaggagaa gaaattaatg ttgttataaa aattagtcat
    10261 ccattttcaa taattataaa ggaaaaagtt tgggttaata atcaacaaat catagaatat
    10321 gatgataaat ctataatgtt taaagctaaa atgagaggtt atgaggagat aaaaagttgg
    10381 attttaagta tgggagctta tgttgaggtt gtagaaccag atagacttag aaatgatata
    10441 ttgtcagaga ttgaaaaaat gaaaaaaatt tactgatttt ttcaaataat ttatacttgg
    10501 accaatatac ttcacggtct agtgttaata tattaattga gagaggtgat attatgaaaa
    10561 tgaaaattga atttagtaca ggttgtatac caatatctta taattcgctc tttatgagta
    10621 ttataaaaga agcaataaga aaatctaatg aagattacta taaaaacatg tactattata
    10681 aagaaaagaa taataaaaaa actaaaaatt ttacattttc ggtttatata aaaaagtata
    10741 gtattgaagg tgacaatttt attatagaag ataaagtaat attaaatata agtactccag
    10801 atttagaatt gggttttcat atatacaatg ggttaatgac ttctaaaaaa tgtttatata
    10861 aagcctatga acttacaaga attagaatcg atttatcaag agaaaagaag gtaacagaag
    10921 aaagggtact atttaatgca ttgtctccaa tctgtgtaaa gtctaaagaa ggaaaattct
    10981 tggagataac tgatgataga tacatagaag aactaaacta tataactaat gaagtggtta
    11041 aaaattatag gggaaatgga ttaaaaagaa agttagaatt tgaaaatata ggtctcaaaa
    11101 agatagtagt taaagagagc ttaagagaat ttaaaaaaat aacaggtaaa gagtatcaat
    11161 atattaatgg ttacaaaggt aaatttatat tgaaaggtga tattgatgat ttaaatctta
    11221 tctacaatct aggaattggt tttagacgtg ctcagggatt tggagatgta gatattttag
    11281 aatggaggtg agataaatga agttaagagt ttatagagga gattggtttt ttaatatggg
    11341 tatagttgga tttttaaata taataaagaa agcagataaa caagctgaaa tatttattat
    11401 ggaagattat atcgaatttg atagcttatt tcttgaaaac tttcatgagt attattttaa
    11461 ttactttatg gatgaatatg atgtatcaaa aagaattaaa aaaaatattg attacagtat
    11521 aaattttatt aaatctaagc ctgatagaat caaagatgga attaagaaaa ttaaagatag
    11581 tgtgaaacaa caaaatgata agattaaaaa atttgatgaa gaaaaattta acctgataaa
    11641 agagaagcta gatagcatgt ctaagataaa aagttatgat gaatttgaca gtttagaagc
    11701 tttagtagat gagactatag atatatttaa aataaaatca ataaatgaca ggcttacagc
    11761 aaatctttat aaatatgtag ttcaagataa ttattttgga caagttagtt tttttaatgt
    11821 tgctaaggcg aaattagatt tagatggatt aaaacagata atgtttaatg attatttaag
    11881 acaaattata tattttggag agttagcaga tttattagaa gagaatgact atgataagtt
    11941 gaaaaactat ttaaatgata gattaaatag tatagctaag gatataaatg aaaaaaaagt
    12001 tagtaagtct agtataaata ctatagaaaa aattatgaag gaaataaata aaaactttat
    12061 aaagaaaaat aaaagcatag aagatataaa agagtatatg gatagtttag aagtatgtga
    12121 aatgtgtggt ttatataagg gaatattaga tgaatactca gaaagtaatt ttgctccact
    12181 tggagtaagc accaataatg ctaggaatat gttttggaat caggcgtatg taccatcaat
    12241 atgtgatatt tgtaagctaa tattgttctg tactccagca ggggctactt atacaagaaa
    12301 gaattattta attaatgaag aaaatgagtt ttatttattt gttaacatgg atacttctat
    12361 aaatgagctt tttgaaagaa ataatagttt aaaagctcaa aaaagtgaat attcagattc
    12421 caaagatgaa aatcctttta atcagcttat aaaaagtata gtagaagaaa atacattaaa
    12481 aagtgaatgg caacttcgta atatactttt tgtggaattt aaagcaagta tagattctaa
    12541 aaaatgtaaa ataaattatt ttaatattcc aacttattta gcaaaatttt ttacagacaa
    12601 gtatgcaaat aaaaaaatac aaagtatcta tgattacaag cttaaatcaa atgtactaga
    12661 tttactcttg aaaaataggg acttgaagca tctaactaat aacattttaa gaaataaagt
    12721 taaaaatgat atggaaagca acaacaaatc aaatatatca ggaatagatt gttttagagt
    12781 agtccaatta agagcattaa taaatagcta caaaaagggg gtatataaaa tggatagtaa
    12841 aaatttggag aagaatgatg aaaagctgag aataatttat tatttaggtt gtgatataca
    12901 tgactatttt gttaataaaa attctaaaaa taaaatagat ggagtaagct ataaactttt
    12961 aaattcagtt aaagtcggaa ataaaagtga ttttatggat acaattataa gagtttttat
    13021 gtcagctgaa aaacagattc cagcatttat tttagatata gaaattgaaa aagatttaga
    13081 ctttgaatct ataggtcatg cttttatatc tggattaatt tctggaaaat atgaaggaaa
    13141 agacaaacta ccaaacgaaa aggaggaaaa ataagatgaa aagagggtta acatttacag
    13201 taatagcaca ggctcaaagt ttaaactatg gggaagggat aggtaatata tcagaattaa
    13261 aaaaattaag tagagatgat ggaaatatat atacatttgc atctcgccag tgtttaagat
    13321 atgacatagt acgattagca gcagaattgt ttaattggaa tttacaggtt gtcgataaag
    13381 aaaaaggtac tattcaattt aaagacaata taagtataag agaatcagaa gaaatggatt
    13441 tatttggata tatgaaaaca aataaaaaag atgagaaaga taaaaaagga ggttctttga
    13501 ctagagaagc tactgtaaga ttaagtaatg caatttccct tgagccatat agaagtgaca
    13561 tggacttttt aaataataaa ggattagcag atagaatagg agaacatcct aatcttgcta
    13621 acatagagca acatttaagt tattatacat atacagtgac tatagattta tctaaaatag
    13681 gtaaggatgg agatatagaa cttgataata aagagaaatg tagaagagta gtagaatttt
    13741 tagaaataat aaaagtattg aatagaaata ttagaggtag acaagaaaat ttatctcctc
    13801 tatttgtagt aggaggagta tatgaaatag caaatccatt tttcttgggt agaattaaat
    13861 taaaaggaga taaaaatgga tttaaaataa ataagcaagc tatagaagaa gtaattcaag
    13921 gtactttttt aggaaaagat ttaaaagaat ttacctatgt tggaatggta gatggggtat
    13981 ttataaataa agaagaattt aagggattgt ttgaagataa ttttttaagt gtagataaat
    14041 tttttggaca actagttaaa gaagtaaagg aatattatgg ggtgaattaa tatgaaggtt
    14101 ttgaaattag atttatttca agaaactgca tgctataaaa aaccatttgc tatgaagatt
    14161 acagagacat atccactgcc tccatattct acaataaatg gattaattca caaaatatta
    14221 aatgctacag aatatatacc atttaatata agtgtacagg gaacatatga aagtatattt
    14281 aataattacc agacaacata tttttataaa aaagatagta ttacatctat gccaatgaat
    14341 agtcatatgc tattgaatgt aaatttgata attcatatag gtggggattt tgatttattg
    14401 gaagaaatat atgatagttt attaaattat gacgagcact tatcattagg tagaaaagaa
    14461 gatttagtaa gaattaatga tattagattt gtagaagtca ataaatttga agtagatgag
    14521 gatatacaat tagatgatta tgaaaattat aagttatcag aatgtaatat aagaatgcct
    14581 atatatatac caaaaacaag tttacaagat acagatatag atggtataag ttacagactc
    14641 aataattact atgataataa tgctgtggaa gataaaaaaa gggtttggaa taaggtagat
    14701 agttattatg ttgaggatgg aaatactata tcaattggag atatattatt agataatgaa
    14761 ggagatttat tatattttaa taatataaat taggagggag agtatgttat atgctaaatc
    14821 taatccagtg gaaacactta gagaacatac tgatgaactt ttaaaacaaa tgaatgtatt
    14881 aagagaaagt tatggaaaaa atataaatag cttagatttt ctagaggaag agatattttg
    14941 gcaattactt gactttgtta tagaatttca tgatattgga aaagcatttt caccgtttca
    15001 agagttaata aaaagcagaa tggatactaa aataaatgaa cctaaaatag taactcattt
    15061 agaaaataat gtaggccata attatctttc tcctgcattt attgattata gttatataga
    15121 caggaaaaaa aataaagaac ttagagcagt attaaatcaa gttattgtat atcatcatga
    15181 aagagatatc tttatagata aagattttaa aaatttaata caaaaaatat tagatgaaga
    15241 tttaataaat aaagtttatg aactacagcg tgaatttaaa gttagatatc caataaaaac
    15301 agaaaaattg agtaaagtat atctacaaag tgtagaaaaa agaatagata aaaatcacaa
    15361 gtactataat ttatatatta tgttaaaagg aatactacat agactagacc atagtgcttc
    15421 tgctcatgaa gttgtggaat gcaataatgt tataaatata ggagaacaga cagaaaatta
    15481 tttaatgaaa gagtttggta gtttgagaga ggctcaatca tttgctaaat ctaatagaaa
    15541 taagaatata atactgattg catctacagg aatgggaaaa acagaaactg ctttgatttg
    15601 gatagataag gataaagcat tttttacact accattaaga gtaagtataa atgcattatt
    15661 tgatagggct aaaaatataa ttggagtagg agaagcaaat gatacattct taggtctgtt
    15721 acattcaaca gcaatagatt atttagaaga aagtaatcag gaaaattcaa gtgagattgt
    15781 agatttagca aagttattat cttgtaaact gacattttcc acaatagacc aaatatttaa
    15841 atttcctttt ttgtatagag gctatgaaaa agtgtactca acattagcat attcaaaagt
    15901 agtaatagat gagatacaag catattcacc agaaatagca gctgtattgg taaaaggtat
    15961 agaaatgata cataagatag gtggcagatt tatgataatg acagctacta tgccaaccat
    16021 atatatagat gaattaaaaa aaagaggtgt aatgaatagt aacttagctg acttgacatg
    16081 taacacagaa aaaataagac attgtgtgtc tattgtagaa aattcaatag atgaaaactt
    16141 aggtaaaata atacaaagtg gaatgaataa aaaagttctt gtaatagtaa atacagttaa
    16201 aagtgctgta gaaaaatatg agttaataga agaaatagtt aaaccaaaag gaatagatgt
    16261 caacttaaat cttttacatt ctatgtatat acaagaagat agagcaaaat tagaaaaata
    16321 tataaaagaa tttgcagata gtgatagtaa tggtatttgg attactacac aattagtgga
    16381 agcatccctt gatattgatt ttgatgagct tcatacagaa aattcaacac ttgacagtct
    16441 atttcaacga tttggtagat gttatagaag tcgtgagtat gaagaaaata gcccaaatat
    16501 atttatatat acagaaaaag ctacaggaat aggtagtatt tatgataagg atattgtaga
    16561 aaaaggactt gagctactga aaacttttat aaatggaaaa gaatgtgtga agatgaaaga
    16621 aaaatataag gtagaaatgg ttaagatact atactctaaa gaaagtctag aaggtacagc
    16681 ttttgaaaag agatttacca gtgctttaaa tattttagat actataacgc catatagttt
    16741 aggttctaaa gatgcacaaa atatacttag aaatattgaa ggatatacag taattccaga
    16801 ggatatatat aataaaattg aagaaacttt aatcaaagat tatgaagagt tgggacaaga
    16861 gcttataaat gcatatagta atgatgatag acaattgata aataaaataa agtctaagag
    16921 aaaaaaagca agaagagaga taattaaaaa aactgtaaat ttaccaatat ataaagctaa
    16981 acaaaatgta attgacatac aggtgaaagg tcttgaagat ttaaaaatac ttttatataa
    17041 atatgatata cacgaaaata agcatacaaa acagatattt ggtaaaggtg tgcttataag
    17101 taatgaaata gaccaattta tttgattttt atattaacaa tgtggctaaa atttactaaa
    17161 gaaggtgatt ttgtgccaat agatttagaa gtattaaaag ttcaaggcgt aaaaataaat
    17221 tattattatg tatgtaaaag aaaactatgg ctattttcta aaggaataac tatggaacaa
    17281 aatagtagta gagtattgtc tggaaagata gtacatgaag attcatataa aagaatgaaa
    17341 aataaagaaa tgttgattga tgatatcttg aagctagata taataaagga cgagtatgta
    17401 aaagaagtaa aaataagttc aaagatgaaa gaagcagatg aaatgcagtt atactattac
    17461 ttatactatt taaagaatag tatgggtata gagaaaaaag gaactataaa ttatgttaag
    17521 gaaaagaaac aagaagaatt gatattgaca gaagaaaaag aaaaaattat tgaaaacaca
    17581 cttataaata taaatgaaat aaatatgcag ttatctcctc ccaaaattga gaaaaaacat
    17641 tattgtacaa aatgtgctta ttatgaattt tgttttgtga aggaggagta acaatgtata
    17701 aagactatta tattataagt aatggaaata taaaaaggaa agaaaatacg atttactatt
    17761 acaatttaga aggtgagaaa aaaatattac cagtagaaca agttgatgat attcatatat
    17821 atggagaagt agatttaaac acaaaactta taaattacat aagtaaatat ggggtgatgc
    17881 ttcatttcta caattactat ggatattatt caggtacata ttatccaagg aagaaaaatg
    17941 tatcaggatt cagtttagta tgtcaatcag cctgctattt agataaaaat gaaagattat
    18001 acttagcaaa atcctttgtg gaaagtgcat cataccatat tttaagaaat atgagatatt
    18061 acaaagttga tgaaagttta attgatacta taaaggatga actaaatgat ttgaatgatg
    18121 tcaaagaaat atcagaactt atgggtaaag aaggcagaat aagaaaaaca tactataaat
    18181 catttaatca aatattaaaa gatgattttg aattttataa aagagaaaaa agacctccca
    18241 aagacccagt aaatactctt atgtcttttg gaaatagcat aatgtatacg aatgttttaa
    18301 gtgagatata taagacacaa cttgacccta caataagctt tttacatgaa ccatcaacaa
    18361 agagattttc tttaagttta gatattgcag aaatttttaa gccaattata gttgacacaa
    18421 taatttttag cctgattaat aataaaagaa ttaataagaa tgattttgaa tatcaagaca
    18481 aaatttgtta tttaagtgaa aaaggaaaga agaaattttt gcaagaattt gaaaataaga
    18541 tgcagacaac tataaaacat agaaatctaa atagaaaagt ttcatataga atgtttataa
    18601 ggttagagtg ttataaatta attaaacatt ttataaatga tgaaaggtac aaagttttaa
    18661 aggtgtggtg gtaagtatgt ttgttattgt tacttatgat attgttgaag caaggtcgtt
    18721 aaatagaatt agaaggatac ttagaaaata tttgacttgg acgcaaaatt ctgtttttga
    18781 aggcaatatt actgatggaa agttacataa atgtatttct gaaatagaaa atattattga
    18841 taatagcgag gattcaatct atgtttatga gataaaaaat cctaattcaa ttaaaaagaa
    18901 atgttatggg attgataagt attctgatga aatgtttata taggtttgca gtgagcgata
    18961 tttatgctaa aataggtgtt aacagttgga atataaggga ttgaaggtgt atgataactg
    19021 ttatcaattg cactactgct cgctcactgc aaattttgat gtttttattg aattataatt
    19081 gcttgattga agtattttca atgtattcaa ttatacctat tttggggttt tatattaact
    19141 aagtggtatg taaatctaac tagatcaaca aggccattcc aaattgattt tagttttata
    19201 ttaactaagt ggtatgtaaa tttaaaggtg caagtcttaa tacttgttac atgagttgtt
    19261 ttatattaac taagtggtat gtaaataaca aagaaccatt tgaatcacga aaactaaaaa
    19321 acagttttat attaactaag tggtatgtaa atttattaac cttatcagaa acctgaccat
    19381 cccaattata gttttatatt aactaagtgg tatgtaaatt ccatttgtct aatgcttcta
    19441 actctttttt tatacgtttt atattaacta agtggtatgt aaattccaaa atgggcttgg
    19501 agttaagagt tgtataagaa acgttttata ttaactaagt ggtatgtaaa ttaattacac
    19561 actccctttt aacgaacata ttttagattg ttttatatta actaagtggt atgtaaatag
    19621 actcgacata gaatcggtta ctaaatccga gggtttcagg ttttatatta actaagtggt
    19681 atgtaaatac taaacctttt tgtttacttt tatttctcat tttacgtttt atattaacta
    19741 agtggtatgt aaatccggat ttttctgctt cgactgttgt tattgagact tgttttatat
    19801 taactaagtg gtatgtaaat agtaattcca tgcaatcttt tatattgctt tttaacctgt
    19861 tttatattaa ctaagtggta tgtaaataga tatacaaggt gaaagtgttg aaatttcaat
    19921 cttgttttat attaactaag tggtatgtaa atattcttag atgttataat ttgtgttgca
    19981 tttaaatcgt tttatattaa ctaagtggta tgtaaataca atattattgt tcatttcatt
    20041 taataattct agtagtttta tattaactaa gtggtatgta aatttgttta tacctttaat
    20101 ctttaaaatt aaatataaaa gttttatatt aactaagtgg tatgtaaatc attgtatact
    20161 aagcttgata tagtgcaaaa tgaaacgttt tatattaact aagtggtatg taaatactaa
    20221 ttgggttaaa caaatggata taactaaatt agttttatat taactaagtg gtatgtaaat
    20281 ccaacccttc gcacgaatcc gtgcgacgaa atataaagtt ttatattaac taagtggtat
    20341 gtaaattgta aaaatattgc ttagcatatt gatatcacta actgttttat attaactaag
    20401 tggtatgtaa atacaagagg tttttcagca atatatgtac ttctataatg ttttatatta
    20461 actaagtggt atgtaaattc atcaatatta attttgggaa tatcaacatt ttttgcgttt
    20521 tatattaact aagtggtatg taaatgcgat aagagattcg agtcttttta ctgggcttaa
    20581 tcgttttata ttaactaagt ggtatgtaaa taggataaag aaaagactca cacaagacac
    20641 agtgtcaggt tttatatcaa ctaagtggta gataagtaga atattagcaa tatcaacgag
    20701 tatttagaaa cttattttat attaataaag cagtatataa aattgtagaa taaacaatag
    20761 catatactaa aacatccacc ttatattaac taaatcatat ctaaataaaa tgcacttacc
    20821 tataaacatt ataaaatcaa tacaaaaatg aggtgaaaca aaatttatga taaagaaatt
    20881 aaacaataaa gacataaata aaatcatgga aatatgggaa aaaagtacaa tcaaagcaca
    20941 tgactttata agtaaagaat actggcaaaa taactacaat actgttaaaa acgaatatat
    21001 acctatatca gatacatttg tatatgatga tggagatgaa ataaaaggat ttataagcat
    21061 aatagataaa agctttatag gagctttatt tatagaaccc aaataccaaa atctaggtat
    21121 cggaggtaaa cttttagatt atgcaactaa aaaatataaa agtctaagtt tagcagtata
    21181 taaagataat aaaaaagcag ttgtgtttta taataaaaaa ggttttaata tagtaaaaga
    21241 acaagtaaat gaagattcag gatttaaaga gtacataatg gaatatagta aataatatga
    21301 ttacattcta ttaaaagaat aattaattta ttttctattt gatttttata agagtaattt
    21361 ttaaaataga ctatatgaag ataaagatat ttattataga tgtaagttaa tcagaattga
    21421 tatagctaca aaagaggaaa ataaaattgt atggtgtatt tcagatttaa caaatttact
    21481 atttaaagat aataagtttg atataatatt aaagatactt tcacctataa actatagggt
    21541 atttgaaaga attttgaata attatggaat gctaataaag gtttgttaaa atttaataat
    21601 tattagatta tagaaaattg ccatgaaatt agttcagcaa gaactagttt catagcaatt
    21661 ttaattctta taagtgtatt tatttttaca gatttaaaaa acttatacag tatctaataa
    21721 tgtaatctta ttactttagc ctataaatat ttaaatctta aaatgttctc tttgagataa
    21781 gtttatttca gagtcacaat ttccacaaca gtaagatgtt gagacatctt taaatttttc
    21841 agtattctca tcaaagttca tttttttctt tgcaataatt cctaccttag ttaaattaga
    21901 accacaatta ttacagtttt gaattttaca taggttatat agtttattaa gtgtaatatc
    21961 ttctaataat tcatcacaat tacaacatct gatagaagaa acttcactag acatttcaat
    22021 aggttcagct tctttagaag aaaatttata tgttatgtca tttgatactt tataacctgt
    22081 atcaaatata ttagaattac aacttggaca taagtacttt ggaagatata tatctttttt
    22141 ttcaaaagta tcactactaa tattacagtt taattcatct aaaattggtc tttcatctag
    22201 gttattacct ttatttaagc ttagaagata attataaatt tttttatctt cattctgttt
    22261 tgatgtatct aacaaatgct ccattatatt agtgtatttt ttgttttttg aaggcatgtt
    22321 taaatccaga taattgtttt caatagcaaa gcatactata ttgaaatagt tatatttaac
    22381 tgcaaaaaat aataacattt catcaaatac tttatctatt attttgtcag aaatacttat
    22441 ccaaaaatct ggtgactcta agtaaacttt cctactaagt tcatctaact cttcatctat
    22501 attatttata ttattttcat taactttcat atcatcactt ccttttaaat atcttgtgta
    22561 taaactattt gttactattt tttatataaa ataaaatctt atataaaaat atatcatatt
    22621 ataatttaca agtatagtct aaatagagct gtttttaagc aaagttttat aaatgactat
    22681 taaaatataa agtaacagta taaagtttac ttataaatta aacgtaaacc taaatacttg
    22741 taatggttga caaaaaaacg tctttatttt atactaaaac aaagctttaa tagaggcttt
    22801 gtatatgtac atataaacta aatgaaataa gacatatgaa gggaattata aaaatgaaaa
    22861 atactaaagt aaatattcaa gatttaacta aaatgtcaat atgtgtagca ttattgtgta
    22921 tatcatctta catatatata ccattgccat ttactccagc aggagtaaca gcacaaacaa
    22981 taatgataaa tttgatagct ttgattttaa caccaagaca agcatttagt acagttggtg
    23041 tatatataat gataggttta attgggattc cagtatttgg tggaggtaca tctggtattg
    23101 gaaagttgtt aagcccaaca ggtggttttt attttgggtt tttgtttgca gcactaatta
    23161 ttagtttatt aaaaggacga aataatgata ttaagagata tatactaata acaatttttg
    23221 ttggaatgcc tattatatat tttatgggaa cagtatttat gtgctatttt aatcaaatga
    23281 cagtagaagc agcacttttt gcagcagtag taccattttt aattggagat acaataaagt
    23341 cggttattgc atctttttta ggcactaaac tgaacaatgt actcagaaga aatctatgat
    23401 aaatctataa tataaggaag ggtgtaatgt atgaaggaag tttttatatt aggaggattg
    23461 agaagtcata taggtctaaa aaatggaatt tttcaatttg ttcaacctga attactagga
    23521 gcaagtgttt taaaagattt aattaaaaag tatgaaattg acaaaattga tgagattata
    23581 tgtggaaatg cagttgggac aggagggaat atagccagat taatgacttt aacagcagga
    23641 gtgtcaaatg aagtacctgc ttttactgtg gatatgcagt gtgcttctgc tatgatgagt
    23701 atagacatag ctttttcaaa ggttaaatct ggtcagtgta atttaattat tgcaggtggt
    23761 tttgaaagta gttctctaca accaatgcgc acatatcata agaatgataa gcgatataat
    23821 acaaataatc caaattatac agttgctcaa ttttctcctg atgataatag tcaaaatagt
    23881 atgctcgaag gggcggaaag agtagcagag ttatatcaaa ttgagagagc agacttggat
    23941 ttttgggtaa aggaaagtca taaaagagca aaagaagcaa gagaagaaaa aatacttgag
    24001 gatattatat ctcctataaa taatagtacc tatgatgaag gtatacgtga taagatgagt
    24061 caaaggttat tagataggat gccaccaatt ttaggaaaag aaactattac aaatgcagca
    24121 aattcatgtc ttattaatga tggagcttca tttattataa tatgttctaa gaagtattta
    24181 gaacatgtaa aaaagaagcc taaaacaaaa attattaata catgcacaat aggaacagat
    24241 gcaaccttaa gtccaacatc agcgatacaa gcaatggata aacttttaga gatagaaagc
    24301 cttaattatt tagatgtatc tgctgttgaa tttaatgaag catttgcagt tatagatgtg
    24361 ttgtttcaaa gaaagtatcc agagttaata gagagatata atatatttgg tggagcttta
    24421 gcgtatggac atccatatgg tgcttcaggt gcaattatta cattacatct tttaaaagca
    24481 ttagaaaaaa ctaagggtag atatggaatt tgctctatag ctgcagctgg tggtctaggt
    24541 tcagctttac tgcttgaaag ggtgtagatt atgaactatt atgaactttt aagaataaaa
    24601 aaagagaggc ataaaaataa aaatttttta attatagatg gagaaaaata tacttatgaa
    24661 aatatattaa gttatagtga gcaactagga aaacaaattt ctttaggtga aaatatctca
    24721 attttaatat attctaaaaa tataatgttt cagttgatta gtttttttgc tattaattat
    24781 tcaaaaaata ttcctattat ttgtcattat
//
LOCUS       DAEPLF010000034        24539 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEPLF010000034 DAEPLF010000000
VERSION     DAEPLF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24539
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.034"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            268..3216
                     /locus_tag="KR314_001254"
     CDS             268..3216
                     /locus_tag="KR314_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0327648.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRREVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            3194..3658
                     /locus_tag="KR314_001255"
     CDS             3194..3658
                     /locus_tag="KR314_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0327649.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3771..4073
                     /locus_tag="KR314_001256"
     CDS             3771..4073
                     /locus_tag="KR314_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0327650.1"
                     /translation="MGVTNVTKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            4135..5499
                     /locus_tag="KR314_001257"
     CDS             4135..5499
                     /locus_tag="KR314_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBH0327651.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            5534..6007
                     /locus_tag="KR314_001258"
     CDS             5534..6007
                     /locus_tag="KR314_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327652.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYINFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGDLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLRFRNKKFENKNI"
     gene            6179..6829
                     /locus_tag="KR314_001259"
     CDS             6179..6829
                     /locus_tag="KR314_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBH0327653.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTFKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            7003..7935
                     /locus_tag="KR314_001260"
     CDS             7003..7935
                     /locus_tag="KR314_001260"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBH0327654.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEDSIFIG
                     KNQNPYQEVHHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYRKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            8209..8922
                     /gene="ispD"
                     /locus_tag="KR314_001261"
     CDS             8209..8922
                     /gene="ispD"
                     /locus_tag="KR314_001261"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBH0327655.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            8919..9404
                     /locus_tag="KR314_001262"
     CDS             8919..9404
                     /locus_tag="KR314_001262"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBH0327656.1"
                     /translation="MRIGLGYDVHKLTENRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    9499..9707
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9771..11486
                     /locus_tag="KR314_001263"
     CDS             9771..11486
                     /locus_tag="KR314_001263"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBH0327657.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDQCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            11608..13053
                     /locus_tag="KR314_001264"
     CDS             11608..13053
                     /locus_tag="KR314_001264"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBH0327658.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKTKGVAVEVDDRDNYT
                     TGWKFNEWEMKGAPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            13493..14974
                     /locus_tag="KR314_001265"
     CDS             13493..14974
                     /locus_tag="KR314_001265"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBH0327659.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLVEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEITEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            15195..15350
                     /locus_tag="KR314_001266"
     CDS             15195..15350
                     /locus_tag="KR314_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327660.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            15495..16892
                     /locus_tag="KR314_001267"
     CDS             15495..16892
                     /locus_tag="KR314_001267"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBH0327661.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKIFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            16877..17290
                     /locus_tag="KR314_001268"
     CDS             16877..17290
                     /locus_tag="KR314_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBH0327662.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            17397..17492
                     /locus_tag="KR314_001269"
                     /pseudo
     CDS             17397..17492
                     /locus_tag="KR314_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase (FAD)"
     gene            17798..18628
                     /gene="thyA"
                     /locus_tag="KR314_001270"
     CDS             17798..18628
                     /gene="thyA"
                     /locus_tag="KR314_001270"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBH0327663.1"
                     /translation="MSLADKIFIDMCNNILENGVSSDGEVVRAKWDDGQPAHTIKKFC
                     VVNRYDLSKEFPILTLRPTNLKAAIDELLWIWQKKSNNINDLNSRIWDSWADETGSIG
                     KAYGYQIGQKHKYKEGEFDQIDRVIYDLKYNPYSRRIITNIYNHNDLNEMNLYPCAYS
                     MTFNVTGNRLNAILNQRSNDILVANNWNVTQYAILVHMLAQISDLEVGELVHVIADAH
                     IYDRHIPLVKELINRESYDAPKLIINPNIKNFYDFRVEDFTLENYKSGPQIKKIPVAI
                     "
     gene            18641..19135
                     /locus_tag="KR314_001271"
     CDS             18641..19135
                     /locus_tag="KR314_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBH0327664.1"
                     /translation="MKVIVAVDLNWGIGYGGKLLQSIPEDMKFFKEKTVGNVVVMGRE
                     TFKSLPGKKALKDRVNIVLTRSKSFNDDRLIVCNSIDETLKELQKYENDKIFIIGGET
                     VYKQFLPYCNELYVTKIQKTYEADRFFPNIDIMSNWDLIKESEVKEYNNIRYVFSTYI
                     NNSL"
     gene            19162..20538
                     /locus_tag="KR314_001272"
     CDS             19162..20538
                     /locus_tag="KR314_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBH0327665.1"
                     /translation="MKKDIFVNRVSNALENDRGAVFVGSGISFSSTEVDWFKLLEPLV
                     NELDITLDNKSDDLPMIAQYIVNNYSGNRGPLVNEISKTFNRKFKLNKYHKYLATTKL
                     STIWTTNYDMLLEDAFYDFDTSVKVSDDSITRNRLEYDVEIIKMHGCISRSDWNEIVI
                     TQEDYDDFSINKPAISQRLSEDLINKSFLFIGYSYRDPNIRNIMINARRLSKKHTQQH
                     YIILKKLKDSDSNITQDDVVRQELWCNDLKRLGISTLFIDEYSELEELLREISQKSRG
                     KTVYVTGSHKESNMNARKLGTKLAKHKDITLLSGQSSGIGSEVVAAFTEECIKSKEDI
                     INRMKIFPNPYSADEKYSDDITLIPDLKKCRTKMLNSTQVVVVFSGGIGTEAEIDVAI
                     KQNCNIIPVITCKEDRENPAIKKILNYSDNMKNIEIKDATYYNKIVDEDGVVTMEEIY
                     ICISKIMN"
     gene            20514..21614
                     /locus_tag="KR314_001273"
     CDS             20514..21614
                     /locus_tag="KR314_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBH0327666.1"
                     /translation="MHIKNNELKHDIIKLKKRNGFIEVGKDKKTLVNCLIGANELEDY
                     KYEIKKIDCILNISEKPDIVSDLSTKRRKISESIWYKIVKDTPFVSATLPIYLVSKNN
                     NKIDSNELLDIIIEQMENGVGMITIHPTANKQIFEDSKNRMVPVTSRGGGMVIQDLIS
                     KNFSEDNVYLKILPEIIKYAQKHNVCISLGATFRSGNIFDSNDRAQITEIKSQIELAK
                     RIYKSNVGVIIESPGHSRPSDIIKISSILKKEGFPIMPLGPIPTDIAIGMDHIAAAIG
                     ATIMGIEGCANIISTVTREEHTGGRPSIESTVESIRTAKIAAHIIDIHQMNDINLDMS
                     VSKSRAKSNTCIFGKDTRYCDRCRELCPLCIR"
     gene            21657..22082
                     /locus_tag="KR314_001274"
     CDS             21657..22082
                     /locus_tag="KR314_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBH0327667.1"
                     /translation="MHRIASFSQQSQRYVKLEQFEYIIPPEIEKIEKAKELFINSMKK
                     YQEDYDKLVEILFENHYNKLIKGGKNEKTSKRQVEKKAIEDARYVFPNACETKMVFTI
                     NARSLFNFFEHRCYENGSCLEGTMTCGDIVQVRKKFKNL"
     gene            22095..22832
                     /gene="rlmB"
                     /locus_tag="KR314_001275"
     CDS             22095..22832
                     /gene="rlmB"
                     /locus_tag="KR314_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBH0327668.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            22835..23362
                     /locus_tag="KR314_001276"
     CDS             22835..23362
                     /locus_tag="KR314_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBH0327669.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            23427..24074
                     /gene="sigH"
                     /locus_tag="KR314_001277"
     CDS             23427..24074
                     /gene="sigH"
                     /locus_tag="KR314_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBH0327670.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            24148..>24539
                     /locus_tag="KR314_001278"
     CDS             24148..>24539
                     /locus_tag="KR314_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBH0327671.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVP"
BASE COUNT        10169 a         2531 c         4540 g         7299 t
ORIGIN      
        1 atccttgtag tttttgttgt acaaaaaaga tagtaatata aaataacatt atcattttag
       61 aatgatttca attgtaatta ggaaaagtga tttttttgtt tttaaaaaaa gtattatttg
      121 taaaatataa aaaaaaattt cttaatattt gaataaaaga atagcttaat ttaaatgtta
      181 ttactttata aaaaaataat aagtacaaaa aaaacaatat atggcatata agttgctata
      241 taaataataa ggagggaaca attataaatg aggcaaagaa taaaaaactt aattgaaaat
      301 gaagataaga aaaatccttt aacagatgaa gcgatttcaa gtaggttaaa tattcgaaga
      361 gaagatgtta cctttttgag aaatgagtta aaaatagaag attcaagaca aagaagaaaa
      421 gttgttttaa ttaaagctat aaaagatatt ttaaaagaag aaaaaactat aaataaaaat
      481 gatataacaa gaagattaaa cagcattggg tttacagtat caagatttac agtcatacaa
      541 tacttaaaag agattcaaga ttctggtgac ctaaaacttg ataaagagag tgatgataaa
      601 aaagaagaaa taatcaataa tagagaaaat aaagatatag catttgataa actcataggt
      661 agtagtggta gcttaagtac aattataaaa ctagccaaag cagcaattct ttatccacct
      721 catggtcttc atactattat tatggggcca actggtgttg gaaagagtga gttagcagag
      781 tgtatgtata aatttgcagt agaatcatcc agattcccta agaatgcacc atttatagtt
      841 tttaatgcag cagattattc agagaatcca cagcttttgc tatcacagtt atttggacac
      901 gttaaaggtg cttttacagg agctgatgag aacaaagaag gattagtatc aaaggcagat
      961 ggaggaatat tgtttataga tgaaatacat agattacctc atgaaggtca agaaatacta
     1021 tttcaactga ttgataaagg tatggtcaga aagttaggag aaacaagact aacacataaa
     1081 atagatgtaa tgattatttc agcaacttct gagccaatag attctcattt acttaataca
     1141 tttaaaagac gtatacctgt aacaattgaa ataccagaat tagttgcaag accattaaat
     1201 gagaggtttg acattataaa taatttcttc ttaatagaag ctcaaagaat gggagcaaac
     1261 atacatataa agtcagatgt attaaaagct cttatgcttt acgattgtat aggtaatgta
     1321 ggtcaactta gaagtgatat tcaagtagct tgtgctaggg gactattaaa ccaacttaca
     1381 aacaaactaa aagaagtaaa tataagcata tcagatgtac ctggatatgt aaaacaaggt
     1441 cttataaaaa tcagaaattg tagaggtaaa atagaaaatt atgtagatgg ggatttaatt
     1501 atagattcaa atcttcaagg tgagatagaa aagaaaaatg aagatatata tacttttcca
     1561 gatgaaatat acaaacttac tgaaagaaga catattgatt tattggaaca aggtttagat
     1621 tttgatgtta taaatagaat cattggagga gaattagaaa gttgcattca aaaatacata
     1681 aaacaagtta aaaaagtttc tattagtaac aatcttccag atataagtcc aattgttgga
     1741 aaatatatcg tagagctaac ttatgaagta atcaaaatag catcacaata cctaagttca
     1801 ctagaaccga atctacattt atgtttagca gttcacctaa aagcagcttt tgagagatta
     1861 aaagaaggaa aagtaatatc aaatgctcat ttgaaagaaa taataggaaa ttatccacta
     1921 gaatattctg tagcagaaaa ggtagctcaa tatttaaagg aaagatataa tataaactta
     1981 ccaaaagaag aaataggtat aatagcaata tattttaaaa tgagtagtag aagagatatg
     2041 tctgaacaaa aaaagatagg tgtacttctt atggctcacg gaagagtagc atcttctgta
     2101 tgtgaggtta caaataaact attaggaatt aatcacgcaa ggtatttaga tatgccattt
     2161 gagaagaagc cggaagaaat gattgaagaa gctactacaa taataaaaga tattgatgaa
     2221 ggtagaggtg tgattatatt agtagatatg ggttcacttt catattttgg agatattatt
     2281 tctggaaaga ctggaattaa tataaggaca atatctagat ttgatacatt gctttctata
     2341 gaggttatac gaagagaagt tttaccagat ttaacaatag acgagatagt tgatgaagtt
     2401 aaaggtatag agccacaagt aaaagaaaag gttagtaaat gtaacaatac aaaagtagat
     2461 tttagaaaac ctataatttt aacattatgt attactggat tagggggagc ttgtaagata
     2521 aaaaaaatcc tagaggaaaa tattaaaagt ataactgaaa aggttgatat agaacctata
     2581 ggtatgattg aatatccaag tcttgattta gagataaaac aaataagcaa tgataaaaat
     2641 gtaatcgcca tagttggaac aattgaccct cagatagcaa atatcccttt ttttagtcta
     2701 gtagacataa atgataaagc aaagttaaat caaataaata acctaattga tacaaatcaa
     2761 attggtgaaa caaataaaaa atatgaatta tcagatttaa tacataaaga agatatattt
     2821 gtggatttac aggttaaatc aaaagaagaa gttctaaaat atatgagtaa aaaacttaga
     2881 gagaaaggtt atgtgacaaa aaatttctat aaaaaagtag tggaaagaga taacttattt
     2941 ccaactgaat tatcagatga aatagcaata cctcacacag atggaataga tgtaattagg
     3001 ccagctataa gtatagccat cttaaagaag cctattgtgt ggagtaaaag taaagtaaaa
     3061 ataatacttt tattagctgt agaccaaaag tgttttaatc cattaagcac tctactctca
     3121 tttatagaaa cagatgaatt taagaagata aaaaacatta aagatagaga ttttattagg
     3181 gagatgatat tagatggagt caatgaaaat aattagtagc aatttaattt ttaaaaatat
     3241 tgaagtgtct aacaatgaag atgcattaaa gtttttggga caacgtttgt ttgacgaaca
     3301 gtatgtaaaa gaaagctaca tacaagctgt tgtagcaaga gaaaaaaagt atgctacagg
     3361 attaccaaca gaaatatacg gagtggcaat accacataca gatatagtac atgtaaatga
     3421 gccaggaata gcaattggaa tactgaataa accagtaaaa tttataatga tgggtacaga
     3481 cgatactgaa attgatgtaa aagttgtatt tatgttagca gtgaaagagc cacaagaaca
     3541 attgcaacta ttagaaaaac ttatgactat atttcaagat aagaatatac taaataatat
     3601 tgttgactta agtgaagaaa gtgtgagtga tttattaaac tctaaattga aaaattaaac
     3661 atgtaaagta tattaaaaat gtaaaattaa acatgcaaag tatgttaaaa aatataaaaa
     3721 gattattaaa gatttctaac tatagatatc tttaaaataa aataatttca atgggggtga
     3781 ctaatgtgac taaaaagata ttagttgcat gtggaacagg agtatgtact tcaacaattg
     3841 ctataaacaa attaaagaaa gcacttcaag acattggtaa attggatatg gttaatatta
     3901 cacaatgtaa agtagtagaa gttgcatcaa aagcacctga ttatgattta ataatttgta
     3961 ctacacaagt atctagtagt ataaaaacac cagtgataaa tggattacct tttttaactg
     4021 gtgtcggaat ggataaatta atcaatgatg tattagaaga gttagaatta taattaaaaa
     4081 aattaaggaa catggtctct aaaatcataa gttttagaga cttaaaatta aataatggag
     4141 ggtctttata tggagatatt aaatttcatt gttggtctag gtgcacaagt aatgatgcca
     4201 ataataatat gtatttttgg tttgatatta ggtacaaaac taggaaaatc tttaagagct
     4261 ggtttaactg tgggagttgg gtttataggt cttaacacta ttatagcttt gctaacagat
     4321 aatttaggtc cagctacaca acaaatggtt aagaatttag ggctaagttt atcaataata
     4381 gatgtaggat ggccagcagc atcagctata gcttttgcat caacagtagg ggctttaatt
     4441 attccaatag gtcttgtggt aaatatagtt atgctaatta ctaatactac tcaaactgtt
     4501 gatgtagaca tatgggatta ctggcatttt gcttttacag gagctttagt ggcaggggct
     4561 actcaaagtg taatgtgggg agtatttgca gcagtagcaa atatggtaat tgtattggta
     4621 atggcagatt taactgctcc aggtatagag gaatatttag gtatgccagg tatatctctt
     4681 cctcatggat ttacacaggc atttgttcca attgctatag tagtaaataa attattagac
     4741 ctaataccag gtataaataa gattgagata aatgctgata cattgcagaa aaaatttgga
     4801 ttatttggag aacctttaat aatggggtct gtaataggtg taataattgg tattgctgcg
     4861 aaatatgata taaaaggaat attacaatta ggtgtaacta tgggagctgt attaatatta
     4921 attccaaaga tggcagcatt attaatggaa ggattattac cagtatcaga agctgcacag
     4981 gaatttattg agaagagatt taaaaacaga ggcaagattt atataggctt agattcagca
     5041 gtaggaatag gtcatcctgt tacattatca gttgcactag tgttggttcc tttgactata
     5101 cttatagcag ctatattgcc aggaaataaa gttttaccat ttgcagattt agcagtaatt
     5161 ccatttgcac ttgtattaat cgtaccaatt acaaagggaa atgtgtttag aacattaata
     5221 ataggaataa ttataattac ttctggatta ttaatcgcaa ctaacttagc tccattattt
     5281 acccaaatgg ctttaaatgc tagctttaaa atgcctgaag gagcaactat gatttcaagt
     5341 atttgtgatg gtgcgaatcc attaagttgg gtatttgtaa aagttatgaa ctataaagtt
     5401 attggaggag tagtttttgg agttatagct ttaggaatgg ctatttacaa taggaataga
     5461 ataataaatg aaaataaaaa gctatctata gaagaataag gttaaaacaa ttttattaag
     5521 gagtgtatag gttatgaaaa aatataaata tgattctaaa aggtacttac tcagtgttac
     5581 aataccagga ttttttatga ttttaatatt gatttatgca ctgtttaata actatataaa
     5641 ttttaatttg aatatatata gtttgttaat aattgtctgt acttatggta tattcaatac
     5701 ttttatatca tcaagtaatc ctaaaaaaat tattattgac agcaaatgta tacattttac
     5761 ctcatttatg actacacata agtatgaaat taaagatata gaaaagataa gaataagaga
     5821 attttataat aaaaaaattt atctaaaaat taatgatgga gaccttttta aaggtaggta
     5881 ttggataaga acaaatatgt ttaataatag tatagcatta tatagttatt tcattgagtt
     5941 ggaagagtgc ttatatccag attcactaag atttagaaat aaaaaatttg agaataagaa
     6001 tatataaaaa taaaaaattg aattgaagcc taacttaatc ttttttgata agatataagt
     6061 ttgaacttta tactaaattt ttagaatttt caatcaagac aataacataa tagctaagca
     6121 attagaacac aactaagatg tataaggcat gtatatgatg aatgggagga tttaattaat
     6181 gttattagag aaattaagaa aagaagtatt acaagcatct ttagaccttt taaactataa
     6241 tttagtgaca ttaacaggag ggaatgtaag tgggagagat gaacaaactg gctatatagc
     6301 aatcacccca agtggaatgg attatagaaa tcttacacca agtgatattg ttatagtaga
     6361 tgtagatgga aatattatag atggaaaatg gaaagcatca gtggatttat cagaccactt
     6421 gtatatatat aagcatagag aagacataaa tagtataatt catacacatt ctacatactc
     6481 aagttgtttt gctattttaa atgaacctat agaatgcgca agtacaactc ttgcaaatga
     6541 agtagggggt tcagtaccag ttgcaaaatt ttcaccacca acatttaaaa agatgggaaa
     6601 atgtgttatt gaagcaattg gtgacaaaag agcatgtctt ttagcaaatc atggcgtaat
     6661 tgcagttggt ccatctgtag gacatgctct tacagccgct gtaatgttag aagattcagc
     6721 aaaagtatat tacttagcaa aatcaattgg cacaccagtg ctactgcctg atgaagaaat
     6781 tcaaagagcg agagatgtat tttttaatgt ctatggtcag gataaataaa ttagataaaa
     6841 ttttaaaagt aatatgaaaa tatagatatt caatgatatt aaggagattt ataatgatat
     6901 aataagttta atagttaaga agtaaaatat gaaatgtaat agtatataaa ttcatttaag
     6961 actaaaaata ataagataaa ataaaaagga gtctgtattt ctatgtatta cttattaaac
     7021 aaaagcacaa atccttactt taatttggca ttagaagaat atctattttt aaatgataaa
     7081 tataatgatg atattataat catatggaga aatgaagact ccatttttat aggtaaaaat
     7141 caaaatccat atcaggaagt acatcatgat gttattgaaa agggagaaat tcctatttta
     7201 agaagaatat ctggaggggg cactgtttat catgatttag gtaatatcaa tatgtcattc
     7261 atacaaaaag ataggcaact tcatgagata gattttttgg agcatactaa gtttatgcaa
     7321 aagatgcttt caaccttagg actagatgta tctataactg aaagaaaaga tttattttta
     7381 aatgggaaaa aaatttcagg ctctgcacag tctataaaaa gaaaaaattc tctttatcat
     7441 ggaactcttc tttacgattc agatttaaat aaattaacta aatatttaaa ttcaaataaa
     7501 gcaactgaat caaatgcaac taaatctgtt tcaagtaaag ttacaaatat taagttttta
     7561 ctcgaaaaag atataaatta ctttttagat tattgtgttg aatatttaaa atctaatata
     7621 aagaatataa aagaacttga attggatgaa gaagatatta aaagtgtata caatatagga
     7681 gaagataaat atagaaagat agagtggaca tatggtaaaa cgccgaaatt tcaagtagtg
     7741 ctaccattta ataatgaaag taaaataaat atacatgtta acagatggaa agtatcaaaa
     7801 ttttttattt cttatgatga taatgtaatt aatatggata agttgttaga tttgaatttc
     7861 tttaaagaag aaataaaaga gagtatttta gaaaattatc cacattacat agatttaatc
     7921 aatttaatat tttaatataa aacctctaat ttgataaaat tgtcttatat caaactagag
     7981 gtttttaata ttcttgcata ttaacaacca ttacaaaatt gtaaaattaa aaacaacact
     8041 attgaaaaat caatatatat tgtaataaat atatgtgttt ttcattagca aatagacatt
     8101 aactatttat acaactgaaa atttaatata taattaatca gatggttaaa ataaaaatat
     8161 taaaagtttt aataaatgat ggttaaaaat aaaatacgag gtgataccat gtacagtgtt
     8221 ataattgttg cagcgggaag cggaaggaga atgaacttag atataaacaa acagttcata
     8281 aaattaagag aaaaagaaat tattgctcat acaattcaag ttttttatga aaatataaat
     8341 atagatgaaa tagtggtatg tataaaaaaa gaagaagagg atttttttaa agaaaatata
     8401 ataaataagt ataactttaa aaatataaaa atagcatatg gcggaaaaga aagacaggac
     8461 tcaatatata atggtttaaa aaaattagat gaaaattgtg atatagtact tatccatgat
     8521 ggagcaagac catttgtcga ccatagaata atcaacgaat ctataaaggt agctaaagaa
     8581 aaaaaggctg tggttgtagg tgttccagta agtgatacta taaagatagt gtcagatgga
     8641 acagtacaag aaactccaga aagaaatttg ttgtgggcag cacaaacccc tcaaaccttt
     8701 gaatataatc tcataataga tgcttatgag caagcatata agaacaatta ttatggtact
     8761 gatgattcaa tgttggtaga aaatataggt caaagtgtca ctatggtaat gggttcttat
     8821 gaaaacataa aaataactag tccagaagat ttaaatatag cagaacaaat attaaatatg
     8881 gaaaaaagag atgaagttaa cagtagaagg agaattatat gagaatagga ttaggatatg
     8941 atgtacacaa attaacagag aatagaaaac ttataatcgg tggtgtagag ataccacacg
     9001 ataaaggttt gttgggtcat tctgatgctg atgtattgat acatgcaata atggattcta
     9061 ttttaggtgc tctagcatta ggagatattg ggaagcattt cccagataca gatgaagaat
     9121 ataagggagc agatagtatg aaattattag aacatgtgta taatctaata acaagtaaag
     9181 gatataaaat tggtaatata gatagtacta taattgctca aagccctaag atggctccat
     9241 atattgagag tatgagaagt aatatatcaa aagttttaaa cacagacatt gataatataa
     9301 acataaaggc aacaacagaa gaaggtcttg gttttacagg agcaaaacag ggaatagctt
     9361 cacaaagtat ttgtttatta ttgttgacaa gtcagaataa ttaatatatt attagtaata
     9421 agtttaatgg atatcaagta ataggcctat atagaatatc tataatgtat gaatttaagt
     9481 gaaattaata taaatataaa attagctatg ataaagaaat agtaaaatga tgaaatcttc
     9541 acagagagaa aacggtggtg agagttttca agaggaagtc tttgaacctg tcttttagct
     9601 taaaggtata aaaatcttta cggtaggaac cgttatattc ccaaaagaga gctattcata
     9661 tagctaacta gagtggtacc gcggaaatta aacctttcgt ctctatactt gtatagagat
     9721 gagaggtttt ttatattttc aggaagttaa tattcaggag gaatctatta atgaaaatgt
     9781 caaaaatgtt tatgccgaca ttaaaagaaa tcccagcaga tgctgagatt acaagccatc
     9841 aattaatggt taggtcaggc atgataaaaa aaatgacttc aggtgtttat aaccaattac
     9901 caatggggtt aagagtgttc aaaaaaattg aacaaataat tagagaagaa ttaaataaaa
     9961 aagattgtca agaaatactt tgtgcagcct tacttccttc tgaattatgg aaagagtctg
    10021 gaaggtggac tgctatgggt gaagaaatgt ttagactaaa agacagaaca gaaagagagt
    10081 actgtcttgg gccaacacat gaagaagcat tcactgatat aataagacaa gaaattactt
    10141 catataaaca attgccactg aacttgtacc aaatacaagt aaaatataga gacgagagaa
    10201 gaccaagatt tggtgttatg agaactaaga cttttacaat gaaggatgca tatagttttg
    10261 atgtagatga taaaggttta gataagtcat atcaagatat gtttgatgca tatgtaagta
    10321 tatttgatag atgtggattg gagaatagcc ctgtacaggc agattcagga gctataggag
    10381 gttctacatc agccgaattt atggttaagt cagaagttgg tgaagatgaa gttgtatttt
    10441 gtagtggatg tgattatgca gccaatgtag aaagagcaga atcttgtaat ctagcatcac
    10501 aaaaagaaga aatgaaagaa ttagaagagg ttcatacacc aggagcagct acaataaaag
    10561 agttagaaga atttttaaaa acttctccag ataagtttgc taaaacttta gtatatgagg
    10621 cagatggaaa aacagttgta gtcgttgtaa gaggagatag agaagttaat gaaatcaaag
    10681 tatctaatgc tataggttct gttatagaat ttgctcttgc tacagatgat gtagtgagaa
    10741 aagtaactaa tgcagaagta ggatttgcag gtccaatagg tataaatgct gactatgtat
    10801 ttatagataa agaaattgtt gaacaaagaa acatagttgt tggggctaat aaaactgagt
    10861 accatataaa aaatgctaac tatggaagag attttgaagg aatagttgga gattttagaa
    10921 atgttcaaga aggcgaccag tgtatagtat gtggtaagcc acttgaaata gctagaggtg
    10981 ttgaagtagg acatatattt aaactaggaa ctaagtactc agaatctatg aatgcaaact
    11041 tcatagacaa agatggaaaa tcaaagccta tagtaatggg atgttatgga ataggtgttg
    11101 aaagaacagc agcagctata attgaacaac ataatgatga gaaaggtata atatggcctt
    11161 tgtcagtagc tccataccat gttgtgatag ttccagcaaa tatgaaaaat gaagaacaaa
    11221 tatcaatagc tgaaaatata tataatgatt tgcaagctat gggtgtagaa gttttattag
    11281 atgacagaga tgaaagaata ggtgtcaaat tcaatgattc agaattaata ggaataccta
    11341 tgaggattac agtaggtaaa aatattaatg aaggtaaagt agaatttaaa cttagacata
    11401 aagaagataa agagataatt gacatagaag aaataaatga aaaagtaaag gcagagttta
    11461 tcaggaataa tgttaggtta ggataataac ttaaataaga tattgtatag ataatcaaag
    11521 ttgtacttta tagacaccat tttatctata tataaggata taaaatggtg tttttttata
    11581 aaacaaataa atgaggaggt aattgaaatg gcaaaaaatg aaaaacaatt tgttgaagaa
    11641 ataacaaaga tggaagatga ttttccacaa tggtacacag atgtaataac taagactgac
    11701 ttagttgatt atgcaccagt aaaaggtttt atggttgtaa agccatatgg atatgcatta
    11761 tgggaaaaga tgcaagaatt tatggataaa aaatttaaag aaactggaca taaaaactgt
    11821 tattttccat tattaatacc agagagttta ttaaacaaag aagcagaaca cgttgaaggt
    11881 tttgctccag aagttgcatg ggtaacacat ggaggaaata aaaaattaga agaaagatta
    11941 tgtgtaagac ctacttcaga gactataata tgtacaatgt atgcgaaatg gttaaaatca
    12001 tatagagaat taccatatct ttataatcaa tggtgctcag ttgttagatg ggaaaaatca
    12061 actagacctt tcttaagaac atctgagttt ttatggcaag aaggtcatac attacatgaa
    12121 acagcagaag aagcacaaga agaaacaata caacaattag aagtatataa agcgttatgt
    12181 gaagaattac ttgcaatgcc agttgtagct ggtcaaaaaa gtgaaagtga gaaatttgca
    12241 ggtggtgaaa gaacatatac tatagaagct atgatgcatg atggtaaagc attacaatca
    12301 gggacaagtc acttcctagg tcaacacttt actaaggctt ttgatattac ttttgcagat
    12361 agagaaggaa atttagctaa tccataccat acatcttggg gagcatctac aagacttata
    12421 ggtggactta taatgactca tagtgataac agaggtttag tattaccacc aagagtagct
    12481 ccaattcaag ttgttatagt tcctatagct gctaaaaaag gaaatgtaat ggagactgtt
    12541 gataaaatat atgctgattt aaaaactaag ggagtagccg tagaagttga tgatagagat
    12601 aactatacaa ctggatggaa atttaatgag tgggaaatga aaggtgctcc agtaagagtt
    12661 gaaattggac caaaagatat agaaaataat caagctatgg tatttagaag agatacttta
    12721 gaaaaggatt ctatgccttt agaaggatta gctgatgcta tatgtgattt atttgatgtt
    12781 atacacaatg atatgtttga aaaagcaaga aaacatagag aagacaatac atctatagtt
    12841 gaaaatatgg atgagtttag aaaagcttta gaagaaaagc caggattcat aaagactatg
    12901 tggtgtggag atgcagaatg tgaagctaag attaaggaag aaacaggagc aactataaga
    12961 tgcttacctt ttgaacaaga aaacttaggt cacaagtgtg tgtactgtgg aaaagaagca
    13021 gatagtatgg tagtaatggc aaaagcttac taaaatacat ttaaataaaa actggggtag
    13081 aaagtattta atactttatc ccagtttttt tgttttttat gagtaataaa atgtatttgt
    13141 aactatgtca taagataagt catagatact tatattaaaa agaatataat agagaactat
    13201 ttttaataat tacatattaa tgaaagcaaa atatttttgt tatatagatt ttcaataaag
    13261 caaacaataa atataaatga atattagtaa ggagtgcttt taatttttag aaaataaatg
    13321 acttaaaaga aagatttatg aaattaaaac taggattttc tgatataata aacatgtgta
    13381 tttttaaata aaaaggatat tattattaga tttgattaaa aataaacttg caaaacaatg
    13441 tactagttta atattatagt tatattgaat aaaatatcag gaggaagtaa aaatgaatgt
    13501 aagagtaagg tttgcaccaa gtccaacagg atttgtacat ataggtagtt tgagaactgc
    13561 tttatataac tatttatttg caaaaaaaat gggtggagaa tatatattaa gagtggaaga
    13621 tacagaccaa agtagattag tagaaggtgc tatagagaat atgcttaatg ctatgaaatg
    13681 ggctggtgtc aaccatagtg aaggtgttat attagatgat agtggaaaag tagtacaaaa
    13741 aggagaatat ggtccatata ttcaatctca aagattagat atataccaag aatatataaa
    13801 gcaactttta gatagtggaa aagcttatta ttgtttctgt acaaaagaaa gactggatga
    13861 agtaagagat gctcaaagag cagcaggaga aacagctaaa tatgatggtc attgtaagaa
    13921 tctttctaaa gaagaagtag aagcgaatat aaaagcagga ataccatatg ttataagatt
    13981 aagattgcca gagaatcata caattaagtt tacagactta gttagaggag atatggaatt
    14041 taatactaat gacctagatg accaagtatt aatgaaaaca gatggatttc caacttatca
    14101 ttttgcagta gttgttgatg actatttgat gaaaattaca catgttataa gaggagaaga
    14161 atgggtatca tctactccaa aacatgttta tttatatgaa gcttttggat gggaagcacc
    14221 tgtatttgtg catttaccaa atatacttaa taaagaaaag aaaaaattaa gtaaaagaca
    14281 aggtgatgta gctgttgaag actttaagaa aaaaggatat ttaccagagg gattagtaaa
    14341 ctatgtagca ttagttggtt ggtctcctga agataatcaa gaattgttta caatggagga
    14401 attagaaaaa gccttctctg tagaaagagt atctaaaagt ggtggagtat ttgatactga
    14461 aaaattaaat tgggttaatc agcactatat aaaagatggt gatgatgctt atctaacaga
    14521 cttagcaata ccatttttag tagaagatgg atttataaca gaagaagaag ctactaataa
    14581 atatgatttc ttaaagagta tgatatcagt acttaaagag aaattacaat atgtaaaaga
    14641 aattactgaa catgcaagta tattttttgg agataaaata gaggtagaaa ctgaagaggg
    14701 aagagagttc ttaagattag aacatatacc tactttaata gatgctttga gagaaaaaat
    14761 agaaaaaaca gaggtattaa atgctgagtt tgttcaagct atgcttaaag aaatacaaaa
    14821 agagtacaaa ataaaaggga aaaatttatt tatgggctca agaataattt tgacagggca
    14881 aatgcatggt ccagatttac caaaagtaat ggaagtttta ggaaaagaaa cttgcttaaa
    14941 tagaatagct tatgtaaaaa ataatatatt atagtcataa aagtccgcta atttagaagc
    15001 ggactttttt tgtgttagta tttggtgaaa atatctgctt aagaaaatga aggtatttaa
    15061 ggtaataaat ttaatatata aaaagcaaag caaaaatata aataaaatat gaataataaa
    15121 ctaataaaat attaagcata gaagaaggga atctattatt ttagtttgat ttaattatta
    15181 agggggtaat tactatgtat agtaatattg atgatgttaa aaaggaatta aaggagctat
    15241 gcttagaata tgttacaatt ttagaaaaat taaaagatga aaaaatgatt acagaagaaa
    15301 cttttgaaaa gtgctcttct caaaaaaaaa tatttttaga agaacaatag aggatggtaa
    15361 tagtattatt attatgtagt ttgaaagata gtacgaaaaa tgatagtaat actattattt
    15421 attgcaattt tcaactataa tataatatat aatgttaaga agataattta taaaaataca
    15481 gtgaggtgat gagtgtgaaa gtttataata cattgactag aacaaaagaa gaatttgttc
    15541 cattagaaga aggaaaggta aaaatgtatg tgtgtggtcc aacagtttat aactatatac
    15601 acataggtaa tgcaagacca tttataatat ttgatactct aagaaggtat ttagagtata
    15661 gaggatacga tgtaacttat gtacaaaact ttacagatgt tgatgataag attataaata
    15721 gaagtcatga ggagggaata tctcctgaag aagttgcggc taagtatata aaagaatatt
    15781 ttgtagactg tgatgggcta gggataaaaa gagctactgt tcatcctcag gttacagata
    15841 atatacaaca gattatagag tttataaaag agttggaaga taagggatat gcatatgcag
    15901 taaatggtga tgtatatttt gatacaaata aatttgaagg ttatggaaaa ctttcaggtc
    15961 aaaaacaaga agatttagag gcaggggcta gaattgaggt taatgaccaa aaaagacatc
    16021 ctatggactt tgttctttgg aaggcaaaaa aagaaggtga gccaggatgg gatagccctt
    16081 ggggagaagg tagaccagga tggcatatag aatgttctgt tatgtctaag agatatttag
    16141 gtgaaacaat tgatatacat gcaggtgggc aagatttaac attcccacat catgaaaatg
    16201 aaatagctca aagtgaagct agaagtggaa aaatattctc aaaatactgg atgcataatg
    16261 gatatataaa tataaatgat gaaaagatga gtaaatcaaa aggtaatttc tttacagtaa
    16321 gagatatatc aaaattatat gatttagaga tagtaagatt tttcatgtta tcagcacatt
    16381 atagaaatcc agtaaacttt agtgatgaga tgttaaatca agctaaagca ggtcttgaaa
    16441 gactatataa tactaaagaa aaattagaat ttacgctaag taatttagta gaatctcctt
    16501 taacagaaaa agaagtagag cttgttaaag aattagatga ctttagacaa aaattcatag
    16561 atgctatgga tgatgatgtt aatacagcag acgcagtaag tgtaatattt gaattagcta
    16621 agttaataaa ttctaatgta gatgaaaatt cttctttgga gtttgctaaa aaatgcttag
    16681 atgaatttaa tgaacttaca ggagtattaa atattgtaaa taagaaaaag gatactgtat
    16741 tagataaaga cattgaagaa ttgatacaaa aaagaactga tgcaaagaaa aataaagagt
    16801 ttcaattagc tgatgatatt agacaacaat tactagatat gggaattgta ttagaagaca
    16861 caagacaagg tgttaagtgg aaaaggatat aaaaatggaa aaaactgaat tagttactat
    16921 gtcaccatta gttttagcat acttaggtga tacagtgtat gagacatata taagagaata
    16981 tttaattaga cagaatacac aaagaaaagt taatgatttg cataaactag ctataaagta
    17041 tgtaaaagct aaagctcagg caactataat tcatgaaatt gaaattgagt taactgaaga
    17101 agagagtaaa atttataaaa ggggaagaaa tcaaaagtca aatacttctc ctaagaatgc
    17161 agatattata gactacaaac atgcaactgg gtttgaggca ttagtgggat atttgtattt
    17221 aaataatgaa atagagagac ttcaatatat tatcaataaa ggaatcaaaa ttattgaaag
    17281 agatatgtaa atattaaaga gtgttgtctt aataataagt aataaggcaa cacttttttt
    17341 ataactaatg tataataaat atatagaaat tagtaatgag aaaggataag aaagttatga
    17401 aagtaaaatt aatatcacat acacctgaac cagaaaaagt aatagctatg gcagctaagc
    17461 tatgttattc accagttggg acagacgaaa tagaaaaaga tttctaaaat atattaaaga
    17521 gcattcctat aacaaatact atggcggttg gaatctatga gaagttatag atatagaatt
    17581 atgtaaatta tttggaaaaa acaggtttga aaattagcca tgttggtatt tctaggttac
    17641 ttaaggaaga tcaaacataa ataagctgag ttgtcttagg tgcactatgt acaacattag
    17701 aatgtgaagt tgaagattta ttacttcaaa cgaacgatgg tactaagaaa aacaatgatg
    17761 aaatgattaa ttaataataa aggtaaggag gtagtatatg agtttagcag ataaaatttt
    17821 tattgatatg tgtaataata ttcttgaaaa tggagtatca tcagatggag aggtagttag
    17881 agcaaaatgg gatgatggcc agcctgctca tacaataaag aagttttgtg tagtaaatag
    17941 gtatgatctt tcaaaagaat tcccaatact tacactaaga ccaacaaact taaaagcagc
    18001 aatagatgaa ttactttgga tatggcaaaa aaaatctaat aatataaatg atttgaatag
    18061 ccgtatttgg gatagctggg ctgatgaaac aggttcaata gggaaagctt atggatacca
    18121 aattggacag aaacataaat acaaagaagg agaatttgac caaatagata gagtgattta
    18181 tgacttgaaa tataatcctt atagtaggag aattataact aatatatata atcataatga
    18241 tttaaatgaa atgaatttat atccttgtgc atatagtatg acatttaatg taactggaaa
    18301 tagattgaat gcaatattaa atcaaagatc aaatgatatt ttggtagcta ataattggaa
    18361 tgtaactcag tatgcaattt tagttcacat gttagcacaa ataagtgatt tggaagtagg
    18421 cgaattggtt catgttatag cggatgcaca tatatatgat agacatattc ctctagttaa
    18481 agagcttatt aatagagaat cttatgatgc accaaaatta ataataaatc caaacataaa
    18541 gaatttctat gactttagag ttgaggactt tacattagaa aattataaaa gtgggccaca
    18601 aatcaaaaag attcctgtgg caatttagaa ggagttattt atgaaagtta tagtagctgt
    18661 agatttaaat tggggaattg gttatggtgg aaagttacta cagtcgattc ctgaagatat
    18721 gaaatttttt aaagaaaaaa ctgtaggtaa tgtagttgta atggggagag aaacctttaa
    18781 atcattacca ggaaaaaaag cattaaaaga tagagtgaat atagtcttga ctagaagtaa
    18841 atcttttaat gatgatagat tgattgtttg taattctata gatgaaactc ttaaggaact
    18901 acaaaaatat gaaaatgata aaatttttat aataggtgga gaaacagtat ataaacaatt
    18961 tttaccttat tgtaatgaac tatatgtaac aaaaatacag aagacatatg aggcggatag
    19021 atttttccct aatatagata ttatgtcaaa ctgggatttg attaaagaaa gtgaagtaaa
    19081 agaatataac aatataagat atgttttttc aacatatata aataatagtt tataggtttg
    19141 tataggagag aaatttaatt tatgaaaaag gatatatttg taaatagagt ttctaatgct
    19201 ttagaaaatg atagaggcgc agtttttgta ggtagtggta tatcgtttag ttcgactgaa
    19261 gttgattggt ttaaattact ggagccatta gtaaatgaac ttgatataac attagacaat
    19321 aaaagtgatg atttacctat gattgctcag tacatagtaa ataattattc aggaaataga
    19381 gggcctttag taaacgaaat atcaaaaaca tttaatagaa agtttaaact aaacaagtat
    19441 cataagtatt tagcaacaac taaactttca acaatatgga caactaatta tgatatgttg
    19501 ttagaagatg cattttatga ttttgataca tcggttaaag taagtgatga ttctattaca
    19561 aggaatagac tagaatatga tgtggaaatt ataaaaatgc atggatgtat ttctagatcc
    19621 gattggaatg aaatagttat aactcaagaa gattatgatg atttttcaat aaataaacca
    19681 gctatatctc aaagattaag tgaagattta ataaataagt cttttctatt tattggatat
    19741 agctatagag atccaaatat aagaaatata atgataaatg ctagaaggct aagtaaaaaa
    19801 catactcaac aacattatat aattttaaaa aagttaaaag atagtgatag taatattacg
    19861 caagatgatg tagtaagaca agaactttgg tgcaatgatt taaaacgttt gggaatttca
    19921 actttattta tagatgaata ctcagagtta gaggaacttt taagagagat aagtcaaaaa
    19981 tcaagaggta aaacagtata tgtgacaggt tctcataaag aaagcaatat gaatgcaaga
    20041 aaattaggaa ccaagctagc aaaacataaa gatattactt tactaagtgg tcaatcatct
    20101 ggtattggct cagaggtagt tgctgcgttt actgaagagt gtattaagag taaagaagat
    20161 attataaata gaatgaaaat atttcctaat ccatattcag ctgatgaaaa atactctgat
    20221 gatattactt taataccaga tttaaagaaa tgtagaacta aaatgttgaa ttcaacacaa
    20281 gttgtagttg tatttagtgg aggtataggt actgaagcag agatagatgt tgctataaaa
    20341 caaaactgta atataattcc tgttataaca tgtaaagagg atagagagaa tccagctata
    20401 aaaaaaatct taaactatag tgataatatg aaaaatatag aaataaaaga tgctacctat
    20461 tataataaaa tagtagatga agatggtgtt gtaacaatgg aggaaattta tatatgcata
    20521 tcaaaaataa tgaattaaaa catgacatta ttaaattgaa aaaaagaaat ggatttattg
    20581 aagttggaaa agataaaaaa acgttggtaa actgtttaat tggagcgaat gaattagagg
    20641 attacaaata tgaaataaaa aaaatagatt gtatacttaa tatatcagaa aaaccagata
    20701 tagtttcaga tttaagtact aaaagaagaa aaataagtga atctatatgg tataagatag
    20761 ttaaagatac acctttcgta tctgctacac tgcccatata cttagttagt aaaaataata
    20821 ataaaataga tagtaatgaa ttattagata taattattga gcagatggaa aatggagttg
    20881 ggatgataac gattcatcca acagcaaata aacaaatttt tgaggattct aagaatagaa
    20941 tggtacctgt cacatctaga ggtggaggta tggtaataca agatttaatc tcaaaaaatt
    21001 tttcagaaga taatgtgtat ttgaaaatat tgccagaaat tataaagtat gctcaaaaac
    21061 ataatgtttg tatcagtcta ggtgcaacat ttagatcagg aaacatattt gattctaatg
    21121 atagagcaca gataacagaa attaagagtc aaatagaatt agctaagagg atatataaaa
    21181 gtaatgtcgg ggttattatt gaatcaccag gtcattcaag accaagtgac ataattaaaa
    21241 tatcgtcaat attaaaaaaa gaaggtttcc caattatgcc actaggacca ataccaacag
    21301 atattgcaat aggaatggat catatcgctg cagctattgg tgctacaatt atgggtatag
    21361 aaggatgtgc aaatataata tcaactgtca cacgagaaga acatactgga gggagacctt
    21421 ctattgaatc cacagttgaa tctataagaa cagcaaaaat agctgctcat attattgata
    21481 tacatcaaat gaatgatatt aacttagata tgagtgtatc taagtctaga gctaaatcta
    21541 acacctgtat atttgggaaa gatacaagat attgtgacag atgtagagaa ctgtgtcctt
    21601 tgtgcattag ataaaaatta tattcaagtt aattcaataa aaaagattgg ataaaaatac
    21661 atcgtatagc tagtttttca caacagagtc aaagatatgt caaattagag caatttgaat
    21721 acataattcc tccagaaata gaaaaaatag aaaaagcaaa agagttattt ataaattcaa
    21781 tgaaaaaata ccaagaggat tatgataaat tagtagagat actatttgaa aatcattata
    21841 acaaactaat aaaaggtggt aaaaacgaga aaacatcaaa aagacaagtt gagaagaaag
    21901 caatagaaga tgctagatac gtatttccaa atgcgtgtga aacaaagatg gtatttacta
    21961 taaatgctag aagtttgttt aatttctttg aacatagatg ttatgaaaat gggagttgtc
    22021 tagaaggaac tatgacttgt ggagatatag ttcaagttag aaaaaaattt aagaacttgt
    22081 aggaggtgaa taatttggcg agtatagaag gaagaaatcc ggtaatagaa gcaataaaaa
    22141 gtgacagaga aatagataaa atattaattg caaattcagc taaagaaggt tcaattaaga
    22201 aaataatagg aatggctaaa gataagaata taattattca gtatgttgat aaacataagc
    22261 tagatgaagt aagtacaagt cattcacatc aaggtgtaat agcttatgca agtgagtata
    22321 agtattatga attagatgag ctaatagatt tagcaaagaa taaagatgaa gacccatttt
    22381 tcattatatt agatgagata acagacccac ataatttggg ttcaataata aggacagcag
    22441 atgcagtagg agcacatgga gttatcattc caaaaagaag gtctgtacac ataactcctg
    22501 tagttgccaa agcatctgct ggagcagtag aatatatgcc agtttgcaag gtaaccaata
    22561 tagttaatac aataaaaaga ctcaaagaag aagggctgtg gattgctgca gctgatatgg
    22621 atggagaaac tttttataaa caaaatctta ctggtcctct tggtgtagta attggaagtg
    22681 aaggttttgg aatatctaga ttggttaaac aaaactgtga ctttatagta aaaatgccaa
    22741 tgataggtaa tgttacatct ttaaatgcat ctgtagcagg tggaatactt ttatatgaga
    22801 tattcagaca aagactggat aaaagtaaat aggcatgaaa aaaaatataa atcattactt
    22861 aataatagat ggctacaata ttataaatgc gtgggacaat ttgaaagaac tggctaaaga
    22921 ggacttagaa gattctcgag aaaagcttat tgatgacatt atagaatttt ctgagtttat
    22981 gggttataag actataatag tttttgatgc ttataatgtt aaaaattctc gagaaaaagt
    23041 tgaaaaaaga aaacacataa caatagttta tacaagagaa caccaaacag cagatagtta
    23101 tatagagaaa tttataactt ctttatcaaa gtatgatgat gtaaaagtag ccactaatga
    23161 ctatgcagaa cagcaaatga ttttaggaaa aggtgctact agaatgtctg caagagaatt
    23221 aaagttagag ttagatcgtt ccaagaataa aatgaaggaa aaaaatatcg gtttgaggaa
    23281 aaaaattcag cgaaattggt tggaggaaag gttagataaa gaaacattgt cgaaacttga
    23341 gaacattcgt agaaagcgtt gaaattacta ggttctttag actataatat acctataaat
    23401 ttgttttata tatgggggag atttacatgt tggtagcaaa agaaaaaagt tatgagttag
    23461 tggacaattg tcagcaggat gagtataaca ttgtattaag agcaagtgaa ggggataaaa
    23521 tagcactaga gtacattatt actaaataca gaaattttgt aaaggcaaaa gcaaagtcat
    23581 attttttaat tggagcagat aaagaagata taatacaaga aggaatgata ggcctttata
    23641 aggcagttag agattttgat ggaagtaaga ccaattcatt taaatgtttt gcggagatat
    23701 gtataacaag acaaataata actgctataa aaactgcaac aagacaaaaa catatacctt
    23761 taaattcata tgtttctttg aataaaccaa tatatgatga agagtcagac agaacacttt
    23821 tagatattat agctacaagt atagtaacag atccagaaga gcttataatt agtaaagaag
    23881 agttaaaaaa tatagaatca aaaatgaacg aattgttaag tgacttggaa ttagaagtac
    23941 tagaattata tttaaatgga aagtcatacc agtttatagc ggacaagctt aaaagagatg
    24001 taaagtcaat agataacgct ttacaaaggg ttaagagaaa attagaaaaa catcttgaaa
    24061 acagaaatga ttaatagtaa aatacttacc ataagcttat tgtgagcaaa attaatttat
    24121 atcgaaagat aagggaggaa attcaaaatg gctaaagcta aatacgaaag aacaaaacct
    24181 catgttaata tagggacaat aggacacgta gaccacggta aaactacatt aacagcagca
    24241 ataacaaaaa cattatatga cagatatcaa ttaggagaag cagtagattt cgcaaacata
    24301 gataaagctc cagaagaaag agaaagagga atcacaatat caacagcaca cgttgagtat
    24361 gaaacaccaa atagacacta tgcacacgtt gactgcccag gacatgctga ctacgttaag
    24421 aacatgataa caggagcagc acaaatggac ggagcaatat tagtttgttc agcaacagat
    24481 ggaccaatgc cacaaacaag agagcatata ctattatcaa gacaagttgg agtaccata
//
LOCUS       DAEPLF010000035        24521 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEPLF010000035 DAEPLF010000000
VERSION     DAEPLF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24521
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.035"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(158..1030)
                     /locus_tag="KR314_001279"
     CDS             complement(158..1030)
                     /locus_tag="KR314_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBH0327672.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTTN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTDAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            complement(1132..1467)
                     /gene="fliT"
                     /locus_tag="KR314_001280"
     CDS             complement(1132..1467)
                     /gene="fliT"
                     /locus_tag="KR314_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBH0327673.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISSKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            complement(1470..2993)
                     /gene="fliD"
                     /locus_tag="KR314_001281"
     CDS             complement(1470..2993)
                     /gene="fliD"
                     /locus_tag="KR314_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBH0327674.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLKVIGNDNLAQQIANDNGIT
                     FANDASGNKVASVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            complement(3014..3370)
                     /gene="fliS"
                     /locus_tag="KR314_001282"
     CDS             complement(3014..3370)
                     /gene="fliS"
                     /locus_tag="KR314_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBH0327675.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELIRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            complement(3370..3765)
                     /gene="fliS"
                     /locus_tag="KR314_001283"
     CDS             complement(3370..3765)
                     /gene="fliS"
                     /locus_tag="KR314_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBH0327676.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            complement(3787..3999)
                     /gene="csrA"
                     /locus_tag="KR314_001284"
     CDS             complement(3787..3999)
                     /gene="csrA"
                     /locus_tag="KR314_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBH0327677.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            complement(3993..4385)
                     /locus_tag="KR314_001285"
     CDS             complement(3993..4385)
                     /locus_tag="KR314_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBH0327678.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDSLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            complement(4401..5333)
                     /gene="flgL"
                     /locus_tag="KR314_001286"
     CDS             complement(4401..5333)
                     /gene="flgL"
                     /locus_tag="KR314_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBH0327679.1"
                     /translation="MRVSTGMMSSNYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            complement(5352..6662)
                     /gene="flgK"
                     /locus_tag="KR314_001287"
     CDS             complement(5352..6662)
                     /gene="flgK"
                     /locus_tag="KR314_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBH0327680.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGNTVSKYDYY
                     NTIETIINEPSDYGISAGIDDFFKGWESLAKDINSDSKKSLVVENASNLATLISESYK
                     KLNKLKGDIDDNINSEIKQINDMLKSLEDLNKSIDVISGSGSTPNDLLDERDRILDDL
                     SFKLDLENSDVKNMLSDGKLELNELKNADGTWKTGVSGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKVNPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESVGLDSQKVNQDEVNQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            complement(6680..7096)
                     /locus_tag="KR314_001288"
     CDS             complement(6680..7096)
                     /locus_tag="KR314_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBH0327681.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDRYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTATYNSYGNIRK"
     gene            complement(7101..7376)
                     /gene="flgM"
                     /locus_tag="KR314_001289"
     CDS             complement(7101..7376)
                     /gene="flgM"
                     /locus_tag="KR314_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBH0327682.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGVKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            complement(7528..7842)
                     /locus_tag="KR314_001290"
     CDS             complement(7528..7842)
                     /locus_tag="KR314_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBH0327683.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            complement(7866..8294)
                     /locus_tag="KR314_001291"
     CDS             complement(7866..8294)
                     /locus_tag="KR314_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBH0327684.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            complement(8303..8971)
                     /locus_tag="KR314_001292"
     CDS             complement(8303..8971)
                     /locus_tag="KR314_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBH0327685.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNSSSGFDMIMMTLLK
                     ALSQNNQQTSNGYNLSSNSENLFDELGSVVNNTASRFIDVDTKDKSVKSRIENAIEQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            complement(9010..9993)
                     /gene="rfbB"
                     /locus_tag="KR314_001293"
     CDS             complement(9010..9993)
                     /gene="rfbB"
                     /locus_tag="KR314_001293"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBH0327686.1"
                     /translation="MKKILITGGAGFIGSNFVHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDSSNYKFVKGDISNREQMYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSPINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLDKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            complement(10031..10588)
                     /gene="rfbC"
                     /locus_tag="KR314_001294"
     CDS             complement(10031..10588)
                     /gene="rfbC"
                     /locus_tag="KR314_001294"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBH0327687.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFCDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVRYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFNYKCTNYYYPEFEHGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICIRR"
     gene            complement(10625..11506)
                     /gene="rfbA"
                     /locus_tag="KR314_001295"
     CDS             complement(10625..11506)
                     /gene="rfbA"
                     /locus_tag="KR314_001295"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBH0327688.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLKITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDMQYGR"
     gene            complement(11615..12487)
                     /gene="rfbD"
                     /locus_tag="KR314_001296"
     CDS             complement(11615..12487)
                     /gene="rfbD"
                     /locus_tag="KR314_001296"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBH0327689.1"
                     /translation="MKILITGSNGQLGKELVSQLEAINQSINQPKYVILATTRNDLDI
                     SNQTNIDNFILHNKPDVIVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LVHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFELKNIDIKVNPIKSNEFKCKAQRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            complement(12693..17774)
                     /locus_tag="KR314_001297"
                     /pseudo
     CDS             complement(12693..17774)
                     /locus_tag="KR314_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
     gene            complement(17785..19317)
                     /gene="purH"
                     /locus_tag="KR314_001298"
     CDS             complement(17785..19317)
                     /gene="purH"
                     /locus_tag="KR314_001298"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBH0327690.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEVLKQKKNLRILKLAKIEDSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKAKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            complement(19310..19903)
                     /locus_tag="KR314_001299"
     CDS             complement(19310..19903)
                     /locus_tag="KR314_001299"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBH0327691.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            complement(19897..20973)
                     /locus_tag="KR314_001300"
     CDS             complement(19897..20973)
                     /locus_tag="KR314_001300"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBH0327692.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            complement(20967..22334)
                     /gene="purF"
                     /locus_tag="KR314_001301"
     CDS             complement(20967..22334)
                     /gene="purF"
                     /locus_tag="KR314_001301"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBH0327693.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            complement(22346..23044)
                     /locus_tag="KR314_001302"
     CDS             complement(22346..23044)
                     /locus_tag="KR314_001302"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBH0327694.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            complement(23050..23526)
                     /gene="purE"
                     /locus_tag="KR314_001303"
     CDS             complement(23050..23526)
                     /gene="purE"
                     /locus_tag="KR314_001303"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBH0327695.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            23976..24488
                     /locus_tag="KR314_001304"
     CDS             23976..24488
                     /locus_tag="KR314_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0327696.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKELSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVKQILDLPVKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
BASE COUNT         7246 a         4639 c         2616 g        10020 t
ORIGIN      
        1 gattttaaag ttttttcaag tattttttct tcatttttaa caatcataac tatactaagt
       61 aacatatata ttttcctttc aaaagtatat ttgtaaaaaa agaaaggagt aaagcaaagc
      121 cttacccttt cttctatatc caatatacaa aattatttta tcctaataat tgtaaaactc
      181 cttgtggttg ctgattagct tgagcaagca ttgactgtga tgcttgaact aatatattca
      241 ttttagataa gttaaccatt tctgaagcaa catctgtatc tcttattctt gattcagctg
      301 ctgtaacatt ttctatagta ttatttaagt tgttttgagt tgattctagt ctattttgtt
      361 gtgcccctaa ttttgctctt gatgtattta attctttaag agctacatct aagctagaca
      421 ccatttgttt agctgcatct gttccagaag ttccatctgc atttaagctt gctattgtag
      481 aacttgttat acctgcttta ctcattatac ttgaagtatt tactagagca acttttattt
      541 cattgttgtt gttagttgtt cctgcaacat ttgttccaaa attagctcca acttgtaatc
      601 ttatttctgt agaacttcca tcaagtaatt tcttaccatt aaactcaatt gagcttgata
      661 ttctttcaac ttcatccttt aattgtaaca actcatcagc tatcttttgt ctttcttcag
      721 cagtattagt ttcatttgaa gattgtactg ataaagttct cattctttgt agtatattac
      781 cagtttcttc taatgcacct tctgctgttt gtacaacaga tataccatct tgaacgtttc
      841 ttcctgcttg gtctaaacct tttatttgag ctctcatttt ctcagatata gcaagcccag
      901 cagcatcatc agcagctctc ttaattctaa caccagaaga taacttctcc atagacttac
      961 tttgtgcatt tacgtttctt cccatttggt tatttgctat caaagcactt acatttgtat
     1021 taactctcat tattttaccc tccttagtat agttgacatc cttgtcattt ctacttttaa
     1081 tcttttttat taattcttca tttatataat cggacaagat ggtacataac tttatacttt
     1141 tttattaaaa atatttactt tttctctatt taagttataa tacattgtgt tcacttgttt
     1201 agtaagctta tactcatgta attctttttt tacttctaat aattgtcctt gtaaaatatc
     1261 tcttatccta ctatcaattt caattaattc cattctatta taaagctgta taaaatcatt
     1321 tctatcaatt tcacttacac tatttataat ctcttgtctt tcacctaact tactacttat
     1381 ctttatatat tcttcctttt caatcaaatt tataatttgt aaagatatat ctttatataa
     1441 atttaatttt tcttttaatt tatccataat taattaccct gtgcttgtga gaaataattc
     1501 atttgggaag aatactgatt caacaaagat tctaaggctg aatatttagc ataaagtctt
     1561 ttctctttat cattcatttt ttcaactaac ctgcttatat ttctttcttg ctctgcaatt
     1621 tgttttgaat atacattttc actagcagaa gcagtttttt ctatacctgc ttttttagga
     1681 agtttagaag ttgaacttcc agaatattca tatacaatat cttttagctt ttcaaacatt
     1741 cccttatcat cataattcga agaataacct gctagtgttt tgtatacctt atcactatca
     1801 tctataagag cctttgaaaa agtatctgca ttaaatgata tttgaccttt tttattgtaa
     1861 ttttcgcttt cattgatacc tagttttctt aatgcaataa tattttttcc atctccaaaa
     1921 aatgcagact gaatatcttc aacaaaacct cttaactcat catcatttct aagtatacct
     1981 tctttagctt tcttttccca tttttctatt tcttcagttg tcatatcttc tttttgggca
     2041 tctgtaagag gcggataatc ttttggtttt ttagtagtaa ctaaaccata gaccttgtcc
     2101 atcagtttat tataatcatc cacaaatgct tgcatatttt taacagcttc ttcagtatca
     2161 gtgacagaag tcaactttgc agttcctttt gaatttacat tatagtcaat attatctata
     2221 ttaaatgaat tttgttcttt acttatatga gttactcttc catgttcatc agttacatca
     2281 gcttctagat tttttccata tacacttgcc actttgtttc cactagcatc atttgcaaat
     2341 gtgataccat tatcattagc aatttgctga gctaaattgt catttccaat tacttttaaa
     2401 ttaatgtctg atgacgaacc agtttgtttt ctcgaaataa tcaattcacc tgtcatctca
     2461 ctataagaag cctttatatc attatctgcg caaagggagt ttatcttact tacaatagta
     2521 cttgtagtat cttgttcaga tattggtata tttacatctc ctatagttaa agaactagct
     2581 ccactggcat ttggagggaa ctgtcgaaca ataggttcac ttgaatttaa tttaatagta
     2641 aattttgctg gttcagccat ttgagaaact gcaaattgat aatttatttt ttctgcacca
     2701 gctgagcctt ttgctactat aatactttca tcagaactgg ttattcttgt agaagagtat
     2761 gctttttcac ttactatact attaggagaa tttacgctta gatatttatc ataaagatct
     2821 tttgattctt gtataacatt tctatatatt tcttgcttcc atttaacgat ttgttgttct
     2881 tgttttgctt tatcaacttt ttccttgtct ctaatcatac tagcttcgat tatgccttcc
     2941 atatcaaaat ttcctgaaag acctgtaact cttataggac ttatacttga cataccttta
     3001 tccctccatc taattatatt tttagttgat tgtaaacttc atgccatgta tctctaattt
     3061 gttttactat tggcaatata tcatcaatta ttttaatatc tttattttca tcagctattg
     3121 ctaattcaga ttttataaat tcataaactt tatacatatc ctttgcccat tgacctgcat
     3181 cttggtctaa tgtagtcatt agttctgtaa atatattttg aactcttata agttcattat
     3241 gtgctttcaa atagtcgtta tttaaaagag cttctttgga tatattggta tattttactg
     3301 tgccatctac aagcatcaat agtaattcct cttttgataa cttatctaat tcagctaaat
     3361 ttaattccat tatttgtacc tacttctttc ttctttactt tttttatcag cttcatgcca
     3421 tgtgtcttta atttgttcta ttacaggcaa cacattatct attacctgga tgtcttttct
     3481 aatattggct cttgaaagtt catatcttac aaaatcataa actttgtaca tatcctttgc
     3541 ccattgtcct gcattttggt ctaatgtaac cattagttct gtaaatatat cctgaactct
     3601 tacaagttct ctatgcgctt tctgtgtatt tttatctaag atagctgctc tggctatttt
     3661 ggtatatttt actgctccat caacaagcat aagtaacaat tgttcctttg atgccataaa
     3721 aacagcattt tgtttataag aattataagg attttctcca tacatatatt acttcctcca
     3781 agcttattat tttaatgact ttaaaatttt tatattttta tttgtagctt ttatattctc
     3841 actctttact ttttcgtata tttcttttct taatatactt atattgttag gcgccgatat
     3901 agctagtttc acattgtttc catctacccc tacaattttt acttctatgc tttctcctat
     3961 aagtactgct tcatcttttt ttcttgaaat tactagcatc cattatcacc tctcaataat
     4021 ggatgcctta ttgaatattt atcatcctgt aaaattattt gcattccaaa attagttttt
     4081 atatttatca ctataggagc tttcatgttc acagttgatt cttcaaatgt ctgaccaaga
     4141 gtaattatat taaatagtac aacatccttt ggagatttca cttctagttt ttctattgtt
     4201 tcctgattta aaactatttc atattcattg ataatctcaa atggagatac aacaacaaat
     4261 cctatacctt cttcttcagt agaattaaat tcttttaaag aatcattatc ttctatatct
     4321 aaaagctcat attcttttaa attttcaaaa cctaatatac ctttttttaa tgtaaccttc
     4381 atcatatctg cgttatttta ctatatgtaa tctaatatag atacattaaa taactttcct
     4441 cctgtttgta gtgaagcttg ataagttaac tctgctgcct ttaattgaac gagtttttct
     4501 ccataatcta tttcttcatt ttgtgacaaa atatccttta actgtatcaa actatcatca
     4561 tttttttcct tcatagcctc tactgtattt gcttttgtcc catatataga cctaacattt
     4621 actgtatttt ctattaattg ttccatgtcg cctaaacaag ttgttgtcag ctcttttata
     4681 gcatcagcat ctggatttgc taatttcttt gatatattat ttaactcttc aaatgggtca
     4741 gtctttgttg ggtcaaatcc aaggctactc aatttaacat tataatctac acttattcct
     4801 tgagctatat ccatactcaa agagtcattt aatttattaa gatttaaact agaattaaac
     4861 tttaagctca ctgaaccgtc agcattatct atacattcaa caggtggtac acctgtattt
     4921 gaacctgaaa atatgtactt tccttcatgt gttgcattta aggcatttgc aatttctttc
     4981 atcttttcat tagtttcatt actaagagtc tttagctcat catctgaata tgcaccatca
     5041 ccacttgcca ccaaagtttt ctttatgtcc gataataatt ttccaagctg atctaatgca
     5101 gtatcagtag tttctaacca attccctgta tctttacaat tctcaacata agtctccata
     5161 gtttttattt cactttttga gttcaatatt tttattgcct tatatggatt atctgatagc
     5221 ttgtttattt ctttcgttgt attaacttgt ctattaacct tatctaatct ttgtagatta
     5281 tcttgtagtg agtttagata gttgctagac atcatacctg tactaactct cattataaat
     5341 tcccccttct attaaattat tccatttatt acaacatcca acagttgcac agctgttgac
     5401 ataacttttg cactagcttg ataggagcgt tgtattttta taagctctgt catttcttca
     5461 tccaaagata cgcctgaaac actcattcgt gagttatcaa tattatttat aattttacgt
     5521 tgattcactt catcttgatt cactttttga gaatctaaac ctactgattc tgcaaaggct
     5581 ttatagtggt cacttattgt acttactttt ccaccagcca ccccaatatc tattttttca
     5641 tcctttaact tagcaatctt tgaagcctca tcagcagtaa gttttaattc atttgggtta
     5701 ctctttatag ctggatttac ctttatgata tcttcaccat ctacatttga agttataaaa
     5761 aaatctgtta ctgttattcc agcagatgta ttataaacat catttatctg cttagctaac
     5821 ccatcagata catcttttaa atcgctttta tatgtatcaa tcttaccatg catttcaaac
     5881 aagccttgta aggttcctga tacacctgtt ttccaagttc catctgcatt ttttaattca
     5941 ttaagttcta atttaccatc tgaaagcata tttttaacat cgctattttc aaggtctaat
     6001 ttaaagctta agtcatctaa tattctatct ctttcatcaa gtaaatcatt tggagtagag
     6061 ccagaaccag atataacatc aatagattta ttcaaatctt caagactttt taacatgtca
     6121 tttatctgtt ttatttcaga gtttatatta tcgtctatat ctccctttaa cttattaagc
     6181 ttcttataac tttcacttat taaagttgct agatttgatg cattttctac cactagactt
     6241 tttttactat cactatttat atcctttgct aatgattccc atcctttaaa aaagtcgtct
     6301 atacctgcag atattccata gtctgatggt tcatttatta tagtttctat agtattataa
     6361 tagtcatact ttgaaactgt attaccatac ttatgagctt ctgctctaaa ttggtaatca
     6421 taaaaactat ttctagctct tgttacatcc gttacgtgta ctccagtccc taattgtcct
     6481 cttgcaattg aattactcaa actaggcata tacattggac tatttgtagc aaactcagtc
     6541 ctttgcctag aataaccgtc tgtatctaga ttacttatat tatgagaaat tgtctgtatt
     6601 gctttttggt gcgcattcat tcctgaatta gctgtattta aaatggaaaa taatcctgcc
     6661 atatttctcc tcctaatatt tattttctaa tatttccata agaattataa gttgcagttt
     6721 gcttatcagc tggttttatg aaattaatca tttttttcgt aaaaataagt tctttcttta
     6781 ttagagaatc atttgattct tgttgatttt tagctaaagc aagaatgtgt ttcaagtctg
     6841 tatatatttc ttttaaatat ctatcattac tattttcaac aacttcactc attgaaatat
     6901 catcaccaaa aagttgtctt ctctttattt ctatggatgc aagctccctg ctaatatctt
     6961 ctagtttagg atttaacata tttaaatttt ctaagtcttt ttcaactata taatcaaatt
     7021 gttcttttaa taattccatc atcttactaa ttatattttt ttcttcatat attataactt
     7081 ttaagtctgt aatcatgact ttatcctcgc atatcctcta tcattttttt cgcaatttct
     7141 tttggtttta ttttgtaagt tccattttct aatgcatttt ttatttcatc gactttttta
     7201 ttcctaatat tctcatcact catatttgac acacttgcca tttcctttgc aaatttagat
     7261 agctctatct tgtctgtttt attaattttt ttaacttctt ccactttttt tacaccagat
     7321 gtattcttta aataattatt cactacaata tttgaactta catttttaat atccatagtt
     7381 tcacctccaa aattacatac ttataattta attatcgctc ataaaataaa aacatttata
     7441 taattcacgc atattttaaa ttttttataa aaatatcccc ttagctaaac tcatacatca
     7501 tagcctaaag ggatactatt taaaatacta atttacatct gaatcttcta ttttagataa
     7561 tcttacagat aacttctcat ttgtaataat gctttctcca taagcaaaca atctatcatt
     7621 tgcatatata tctaattttt catccacatt cttacttagt tttattatag agccttcttt
     7681 taaatctaat atatttttta ttgtttctgt tgtatttcct atagaaacag ctacatttac
     7741 ttttgcatta aaaacatttt gattttcttc tataacacca gatttttctt ctattaattg
     7801 cccaaaattc aattcatata tatcctcttt tttactattc ataaaacttt tctccttgta
     7861 taaccttatc tatacatatc tatcaacaag ccttgtatca tatcaacagt agaaacaata
     7921 tctatgtttt ctttttcttc actaattaac ttactagtca tatcctcaag tttactatca
     7981 acaatcttta cattcttata atactgatgc tcccaaaagc tactattttt atcaagtgaa
     8041 tacatattat ctacaacaga ttttaggtat tcttttactg catttttata tatactaatg
     8101 tcgccatagt tttgagtaag tacaagcctt tctcctgcct tttttacact ttccaaattt
     8161 cgttttatat cttccaatga agaagattgt tttaaagagt ttaatttgtc agaaaattta
     8221 ttactctctt ttagtttttt aacctccata tttatacttg gacttatgtt tagcttatct
     8281 atatttatcc ccataataaa gcctaccctc tataatattt cataacctta ggaatataat
     8341 tttgtgtttc ttttggcatt ttatacaggt cagagattga ttctactcct ctacttttca
     8401 ttcttccagg accaccatta tatgccatca atcccatttc tatgctccct ccaaacatat
     8461 caatgtattc cttaatatgt ctagttccag catctatatt ctgctctata ttaaaagggt
     8521 ctgtaacccc caagtctctg caattttctg gcataagttg cataagaccc ttagcaccag
     8581 cagaagaaac agtatttgga ttaaaatccg attcaacttt aattattgct tttataagat
     8641 tcgaatccac attatatttt tttgatgctt gctctatagc attttcaatt cttgatttta
     8701 cacttttatc ttttgtatca acatctataa atctactagc tgtattattt actacactgc
     8761 cgagttcgtc aaataaattc tcagaattag acgataaatt gtagccatta gaagtctgtt
     8821 gattattttg tgataatgct tttaacaagg tcatcataat catatcaaat ccagatgaac
     8881 tattatatgt acagttacat tgatttgtag tattactacc agataattga gtcaatgcta
     8941 atatattaat tagtgaactt atctcattca atataaaacg cccctttcta aattttataa
     9001 atttcaaagt tacactgata aaacagcttt ccaccaagat tcattttcta tataccactt
     9061 aacagtttct tttataccca aatcaaagtc atattttgcc ttccaaccta actcattctc
     9121 tattttagtt gcatcaatag catatcttaa atcatgacca ggtctatcat ttacatagga
     9181 tattaactct tcagatttat ctaaaagatt taatatcatt tttactactt ctaaattact
     9241 tctttcatta tgacctccta tattgtaaat ctcccctata ttccctttat gtattattaa
     9301 gtctatagca gtacaatggt catatacatg taaccaatct cttacatttt ccccattccc
     9361 atatactggc aattgtttgt tttccaaagc atttaaaatc ataagaggta tcaatttttc
     9421 aggaaaatga tatggcccat aattgttgga acacctagat atagttgtta acaatccata
     9481 tgttctataa taagaactta ccaacaaatc tgctgatgct ttagaagctg aatatggaga
     9541 acttggattg ataggagatt gttctgtaaa gaataaatcc tttctatcaa ttggtaaatc
     9601 accatacact tcgtctgtag atatttgatg atacctttta actccatatt ttatagatgc
     9661 atctaaaagt acttgtgttc ctataatgtt tgttttaata aataaatcag gattctcaac
     9721 tgacctatct acatgagatt cagcggcaaa gttaacaact atatcaaacc tttcttcttc
     9781 aaatagctta tacatctgtt ccctatttga aatatctcct ttaacaaatt tataattgga
     9841 actgtcttgt atttctatta atgtctctaa attaccagca taagtcaaca agtctaggtt
     9901 aacaataaaa taatctacat acttgttaag catataatgt acaaagttac tcccaataaa
     9961 tcctgctcct ccagttatca gtattttttt catatttttt ctcctaaatg ttaattctaa
    10021 taaattttta ttaacgtctt atacaaatat ctttaaaact tttttgtttt ttgtcctttt
    10081 ctgataaaaa tatttcttca attcctttta aaggccactc aatagatatt tgcttgtcat
    10141 tccatataaa cccatgctca aattctggat aataatagtt tgtacatttg taattaaata
    10201 ctgctttttc tgataaaact aaaaatccat gtgcaaaccc ttctggaata taaaattgtt
    10261 ttttattctt atcagacaat attacacctt cccatttccc aaatgtctgt gaatcttttc
    10321 ttaaatcaac tgcaacatcg aaaacttctc cctctgtaac tctgactaat tttccttgag
    10381 gatatctaac ctgaaaatgc attcctctta aaacaccttt tcttgagcta gactcattat
    10441 cttgaataaa acacatatca agaccctgct ctataaaatc ctttttatta tagctttcca
    10501 taaaatatcc tctttcatca caaaatacat ttggttcgac tatatataat ccttctattt
    10561 tagtctttat aacattgatt tttcccatta taacaaactc ccttaataaa atttataata
    10621 aattttatct accatattgc atatcttcac tagccaattt taatagatat tgaccatatt
    10681 cagtttttat cagtggcttg gctagtttca ttaattgttc tctacttata tatccttttg
    10741 tatatgcaat ctcttctaaa catgctatat acagtccctg tcttgtttga accacttcaa
    10801 catagttaga agcatccaat agacctttat gtgtaccagt atcaagccaa gccatacctc
    10861 ttcctagaag ttccactttt agttttcctc gttttagata ttcactgttt atagaggtta
    10921 tttctaattc acctctatct gatggttcaa ctagctttgc tatctctaca acatcattat
    10981 cataaaaata taatcctggt atagcataat tagatttagg aactgtaggt ttttcttcta
    11041 ttgataatac attaaaatcc ttatcaaatt ctacaactcc aaacgaattt ggattactta
    11101 catgataccc aaatatagtt gctccttttc ttttaactgc attctccaac ctttcactaa
    11161 atccatatcc ataaaatata ttatccccca atacaagaga cactctgtca tttcctataa
    11221 attcttctcc tattataaac gcttctgcaa ttccatttgc tttctcctga attgcatatg
    11281 ttattttaag ccctagtgaa cttccatctc caaataattc ttcaaatgct tttatatctc
    11341 ttggtgtaga tattattaat atatcttgta tccctgccaa cataagaacc gacatagggt
    11401 aatatatcat tggtttatcg tatattggaa gtgtttgttt tgatatacat tttgttatag
    11461 gatatagtct tgtaccagac cctcctgcta aaataattcc tttcatcttt ttccatcctt
    11521 aaatttgttt ttgtcatatt atgattatcg gataaataaa agaaatattt atatttttta
    11581 taatatctta ttttcttttc taaaaataat acatttactt ttcatttata tcatatcttt
    11641 ctaaatattc ttcaatggat tcttcccact ttctaaaaga atttaaacca attaacttca
    11701 acataaaatt atccaataca gaatatagtg gtctttgagc cttacattta aattcgttac
    11761 tcttaattgg atttactttt atatctatat tttttaattc aaatatcttt ttggcaaaat
    11821 cataccaact acactcacct tcacatgtag catgataaac tccataatgt tctgtttcca
    11881 tgattcttat tataattttt gctaagtcta ctgtacttgt tggggttcca aactggtcat
    11941 taaccacatt aacctcttta ttttctaaag aaagttttat catagtcttt acaaaattat
    12001 tcccattacc atataaccat gctgttctca atataaagta cttatgtgag aattgttcaa
    12061 caaacttttc tcccataagt ttactttttc catacacaga attgggttca gttttattgt
    12121 cttccctata tggatattta gaaaaaccat tgaatacata gtcagttgat atgtgaacaa
    12181 gcttagcatt aactttttct gatgctattg ctaaatttct cactcctaag gcatttattt
    12241 tatacgcaat ttcgatatta tcttcacatg catcaacctt tgtatatgct gcacagttta
    12301 caattacatc aggtttatta tgcaaaataa aattatctat attagtctga ttggatatat
    12361 ccaaatcatt tcttgttgta gctaatataa cgtattttgg ttgatttata ctttgattga
    12421 ttgcttctaa ttggctaacc aattcttttc ctaattgacc attagaccct gttattaata
    12481 ttttcattaa ttatgttgcc ctccaataaa tatttaattt tacttagtaa ttaattataa
    12541 cacagcttgt attttaactt aggttgaact tattatttct tagataaata aaaaagagat
    12601 gtaatattaa atgattatca tcatattatt acatctcttt aaatctaaat atctaaatta
    12661 tatattctag ctatcaatta aacatcaaag ctttatctaa aatatttaac tccagattca
    12721 aatatctttt ggtctttttc acctataata ttcttaaata cttcctcacc aattctctca
    12781 gaatgtccca ttttaccaaa cactctacca tcagcactag ttataccttc aacagcatag
    12841 aatgaaccat ttggattaaa ctcaatatca taagtagctt cattattaaa gtcaacatat
    12901 tgagttgcta cttgtccatt agcaattaat tctctcataa catcttcact agcaacaaat
    12961 ttaccttctc catgagaaat tgctatatta tgaatatctc caacattagt ttgagctaac
    13021 caaggagatt tattagatga aattctagtt ctagcaatct tagcttgatg acgaccaata
    13081 ttattgtaag taagtgttgg agcactttca cttggaactc ttatttctcc atatggaact
    13141 aatcctaatt taattaatac ttggaatcca ttacagatac ctaacattag tccatctttt
    13201 tgagttaaaa attcatttat agcttcttgc actctaggat ttctaaatac agtagctata
    13261 aatttaccag aaccatctgg ctcatcacca gcactaaatc cacctggtaa cattattatt
    13321 tgagaagatt ttatttcgtt tactattgta tctatagaat cttctatgtc tttataagtt
    13381 aaattcttga atactcttat gtttgcatta gctcctactc tttcaaatgc tcttgcagag
    13441 tcatattcac agtttgttcc aggaaatgct ggtataaata ctcttggagt agctatactt
    13501 aatgatgatt ttttagcttc tccttgtgat atataactta tagtctctat ctttgatttt
    13561 acttcttctg ttttagttgg gaatataggc tctaaagtac tacagtgtgc tttatataat
    13621 tcatctaatg caagttcttc acctttgata aatatactag cttctttagt tgtacttcca
    13681 agcacaacat aattgtatcc ttctaacata cataaatcat catttgctaa ttcaagtact
    13741 atatttccat aactagcctc aaataattta tcatctgtaa atgttttttc agcttcttca
    13801 ctaaacttaa atcctatttt attaccaaaa gccattttac ttattgcttc acctacacct
    13861 gcaaatccta atgcatatgt tgataaaact ttatttccat gtataagttc tcttactttg
    13921 tctaagtttc tctttaattc ctcaaaatct acaacatcat tttctgcctt gtttacacat
    13981 aacattacta cttttgagtc tgctttttta aattctggag atactacttt cttcgcatct
    14041 actgtatcaa cagcaaatga aactaatgtt ggtggaactt cgatatcttt aaatgtacca
    14101 gacatactgt cttttccacc tatagcaggt atctcaaatt tactttgtgc ataaaatgct
    14161 cctaaaagtg ctgagaatgg tttaccccat ttttctggat tgttaccaag tttttcaaaa
    14221 tactcttgga atgtaagtct tgttgtacta taatttcctc caatagcaac caatttacac
    14281 acagattcta ctactgcgta caaggcacca tggaatggac tccattttcc tacctttgga
    14341 ttatatccat aagtcattat tgtagaagta ttagtttctc ctccaagtac tggtatctta
    14401 gcaaccatac cttgtgttgg agtcgcttga tactttcctc caaatggcat aagtactgta
    14461 ttaccaccaa ttgtattatc aaacatctct actaatcctt tttgagaaca aacattaagg
    14521 tcagataata cttttgtaaa cttgtctttc atacttgcac acttaacatc ttttactatt
    14581 tcattagagc taaagaatgt attttcttca tctactttag taacatggac atcagtagtt
    14641 tgttttactc cgtttgtatc taagaactct ctatttatat ctacaattgc ttttccattc
    14701 cagaacattc ttaaatatcc tgtatctgtt actgtagcta catgtgtagc ttctaagttt
    14761 tcttctactg ctaattgtat aaatttatct ttattttctt tttttatagc tactgccata
    14821 cgctcttgtg actctgaaat tgcaagctca gttccatcaa gtccatcgta ttttttaggt
    14881 actaagtcta agtttatatc taaactatca gcaatctctc ctatagcaac acaaactcca
    14941 cctgctccaa aatcattaca tctttttatc atttgagcaa cttctttatt tctaaagaat
    15001 ctttgtattt ttctttcgtt aggagcatct cctttttgaa cttctgcact acaagttaag
    15061 attgactctt cactatgttc tttagatgaa ccagttgccc ctccacaacc atctcttcct
    15121 gtttttcctc caagaagaac tattacatct cctgcttcag gtctttctct tactacattc
    15181 tcttttggta ctgctgctat aactgcacct atttccattc tctttgcaac aaagttttca
    15241 tcatatactt ctgcaacttg tccagttgta agacctattt ggttaccata tgaactatat
    15301 ccatgagcag cttctgtagt tatttttctt tgcataagtt ttccaggtaa agtatcttct
    15361 aaagtcgttc ttgggtcagc acttcctgta acacgcattg cttgataaac ataacttctt
    15421 cctgataatg ggtctcttat agctccacct aaacaagtag ctgctccacc aaatggttct
    15481 atttcagttg gatggttgtg agtttcattt ttaaacatta ctagatattt ttccatctta
    15541 ccatcaactt caacatctac gtttatacta catgcattta tttcttcact ttcatctagg
    15601 tcatttaatt tcccattctt ctttaattct tttactgcaa cagtggctat atccattaaa
    15661 catatatctt tatgtctgtt cacatataca ttgtctcttg agttaagata catttgatac
    15721 gcttctttta ctatatcatt aaacttacca tcttctatct ttacattttc tatttcagtc
    15781 ataaaagtag tatgtctaca atgatcagac caataagtat ctaatacttt tatttctgtt
    15841 atagttggat ttctcttttc tgtatcttta aagtattttt gaacgtgttg taagtctcct
    15901 aaagtcattg ctaatccttt ttcacttaca aatgttctta atccattttc atctaaatct
    15961 ataaatccat ctaatacttc aactgttgtt ggtatttcag tttccatttt taatgtttct
    16021 ggtttttcta aagatgcttc tctactatct actggattta tacagtaatc ttttatttta
    16081 gaaaactctt catcagttat tttgcctgat aatatataaa cttttgctgt attaatagca
    16141 ggtcttatcc cttgatttac aatttgtaca cattgagctg cccaatcacc tctttggtcg
    16201 tattgaccag gaagaaattc tattgcaaat actctttcat catttaattt tggtatttct
    16261 tcataatata ctacatctaa atttggttca gagaatattg tctttgctgc attttcataa
    16321 acttcttctg atattccttc tacatcatat ctatttaata ttcttaaatt ttctatatta
    16381 tctatgtgta aactttctac taagtctttt tttaaagctt tagcctcaag gtcaaaacct
    16441 tctctttttt caactaatac tcttctaacc attgagtctt tattttcagt attcaacata
    16501 ccttatcctc cattttagaa aaattatctt cctatgtctg ttctatagtg cattccttca
    16561 aagtttatct tctttatgtt ttcataaact ttttctgctg catccttaac tgtagttgac
    16621 tttgcagtta taccaattac tcttcctcca ttggtaacta aattaccatc aagcatctta
    16681 gtgccactat ggaatactac tatatcatca tccaagtttt caagaccagt tataattttc
    16741 cctttttcat aactgtctgg ataccctcct gatgtaagca taacacatac agcttcatca
    16801 tcactatagt ttatttctat atcttttaac ttattatcta gtatagtttc cattatctca
    16861 tgtaaatctg tttctaatct aaataaaaca gattgtgtct cagggtctcc aaatcttaca
    16921 ttatattcaa gtactttttc tccatcttca gttatcataa gaccaacaaa taaaatacct
    16981 ttaaaattaa gtccatcttt tttgaagcct tctaaagttt tttctaatac tgtctctttt
    17041 actttatttg ctaattcttc tgtatatatt tcactaggtg aaaaagttcc cattccccca
    17101 gtgtttggac cttgctcata gttgtttact tttttatggt cttttgcact tgccatagga
    17161 actattgtat cattgtctac aaatgctaga attgaagttt ctattccttt taaaaactct
    17221 tcaattacta ttttatctcc agcagctcca aacttcttat ctgacatcat ttcttttaaa
    17281 gttgctatag catcttctct attttcaggt attacaacac cttttccagc agctaatccg
    17341 tccgctttta ttactactgg atatccaaaa ctatctattt ctgatatagc ttcttctaag
    17401 tttgtatatt ctttatattt tgcagtaggt atgttatgtt ttatcataaa ttcttttgaa
    17461 aaagctttac ttccttcaag ttgagcacat tttttattag gtccaaatac tcttaatcct
    17521 tctttttcaa actcatctac tattcccata actagtggta cttctggtcc tactattgtc
    17581 agacctattt cattttcttt ggcaaatttt aaaagttctt ctatattagt atcacctata
    17641 tttacacatt ctgctacatt tgtaattcct gcattacctg gcgcgcaata aactttttct
    17701 acattttttt ctttgcttag tttccagcat atagcatgct ctcttccgcc accgcctaca
    17761 accaatatct tcataatatc ctccttagtg tttgaagtgt cttattcctg taaataccat
    17821 aaccatatca tacttatcac aagcttctat tgaatcttta tcctttatag agccacctgg
    17881 ttgaactact gctgatattc catattcatg agctaatgtt acacaatcat caaatgggaa
    17941 aaatgcatct gacgctaaaa cagctcctgt aaaatcttta tctttgttat tttcaagagc
    18001 attttttaat gcccaaattc ttgatgtttg tccacaacct aaagctaaag tttgaccatt
    18061 ttttactatc gcaattgcat ttgatttcat attttttact actctcatac caaattccat
    18121 gtccttaagt tgtgcatctg ttggttttgc ttttgttaca ttttcacttt tttctgctaa
    18181 tatattattt ctatcttgta ttaataattt tccatctaaa tatttcattt catatggttg
    18241 taaactatct tctatcttag ctaattttaa tattcttaaa tttttctttt gtttaagtac
    18301 ctctaaagct tctggctcaa aatcatatgc tactactatc tctaagaata tttcatttaa
    18361 tttctctgct gtagctttat ctatagtaga agttattcct actattcctc caaatattga
    18421 aaccttatcc gcttcataac attttgtata tgcttcaaaa gcatcttttc ctagacctac
    18481 cccacatgaa ttagcatgtt ttatagctac tgaaactact tcttcactat ctttaaattc
    18541 tctcattact tctaaacatc catgtaaatc atttaaattg ttaaaagaaa gttctttacc
    18601 gcctaattgc tcataattta agattggatt ttttgcattt gattgtgaat ataagaaacc
    18661 attttgatgt ggattttctc catatcttaa agtttgttct ttttggaaag ttaaatttaa
    18721 tatgtcagga tatgtatctc caacttctcc agcaaaataa ctagatatta acgcatcata
    18781 tcttcctgta gttgagaaag ctttatatga taattttttt ctgtcttcta atgtcattgt
    18841 atctgttttt aatttttcta ttatagaatc ataatctttt atatctacta ctactattac
    18901 atctttatag tttttggcag cagaacgtat catagatggt ccaccaatat ctatattttc
    18961 aattatttca tcatgacttt ttccagcttt taaagttcct tcaaaatcat atagatttac
    19021 agctactaaa tctattgaat gtattttatg ctcattcaca gtttctacat gagattcttt
    19081 atctctttta taaagtatac caccatgtat atatggattt aatgttttta ctctcccatc
    19141 taatatttct ggaaaatttg taacatcttc aacttgcatt acattaacac cattttcttt
    19201 taatgtttta aatgtatttc ctgtagatat aatttcataa tctaacttat ttaactcctt
    19261 agcaaattcc actacaccag tcttgtcagt tacacttatt aaagctctct tactcattta
    19321 taaacactct cctaccttga agttttaatt tattttcaca aaaaagactt atactttctt
    19381 ttaaaatcct atgttcaact tctaacactc tttttgcaag tgtctttgca tcatcatctt
    19441 gatttacctt aactacatct tgcattatga ttggtccagt atcagcacct tcatcaacaa
    19501 aatgaactgt agcaccagtt actttagctc cgtaatctat aacaccttga tgaacttttt
    19561 ctccatagaa cccagctcca caaaatgaag gaattaatga tggatgtatg tttatcattt
    19621 tgtttctaaa ctcattaacc agctttgggc tgattatttt taaataacca gccaaaacta
    19681 ctaaatctat tttattttcc ttaagaatct ctattatctt gtcttcatca gtttcacaaa
    19741 ttgcttttat gttatgattt ttagctctct ctagcccata agcaccttgc ttacttgaga
    19801 taactacttt aacctgacct tttatttctc cagactcagt gccatctata actgcttgta
    19861 aatttgttcc tccaccagat attaaaactc ctatatttag cataattcta caccttcatg
    19921 actatctact acttcaccaa ttatatacgc tttatcttct aataattcac tgtatttttt
    19981 atctacataa gcagcattgt cattttctct attatttata aagtttacaa catcatcagc
    20041 attttcttta tctactatta aaactagccc aatacccatg ttaaaactct tatgaagttc
    20101 tctctcatca acagcattaa aaccttctat cattttaaat ataggaggct tctcccaaga
    20161 ttttttattg atatctagtc ctaacccttt aggtataact cttgttatat tttctataag
    20221 ccctccacca gttatgtgag ctatagcttt tatgtcatgt ttagctaata catctaaagc
    20281 taatttaaca tatattttag ttggtgttaa aagtgcttct ccaactgtca ttcctagttc
    20341 ttcaacatat tgttccattt tataattgta agtttctaaa aatatctttc ttataaaaga
    20401 aaatccattg ctgtgtactc cacttgaaga tataccaacc aatacatctc ctgatttaac
    20461 attgtttcca gaaacaatct tttctttgtc tgctattcct acagaaaatc ctgctaaatc
    20521 atagtcatct tctccataca ttccaggcat ttcagcagtt tctccaccaa ttagtgcaca
    20581 accagacatt ttacaaccat ctgcaattcc accaactatt ttttcgatat gctcaggcac
    20641 taatttccca agagctatat agtcaaggaa aaataatggt tttgctcctt gacaaattaa
    20701 atcattcacg cacattgcaa ctaaatctat tccaacagta tcatgcttgt ccatcatctg
    20761 agctattttt aacttagtcc caacaccatc tgtagatgca agcaaaactg gttctttcat
    20821 acctacaaaa tcttttaaac tgtataatcc actgaagtta cctaaatctc caactacatt
    20881 tccatcataa gttcctttta ttttaccttt tataaggtct actgctctat ttccttcatc
    20941 tatatctaca cctgattctt tatatgttaa catgaaagta gttcctcctt atcaattttc
    21001 ttaactgggt attctccatc aaaacaagct ttacagaaag tagacttatg ttctgttgca
    21061 tctaacattc cttctatatc taggaatttt aaactatcac atccaatata ttctctcatt
    21121 tcttctacgt tgttacttga tgcaattaat ttagagcgat ttggagtatc tattccatag
    21181 taacaagagt aagctactgg aggagaagtt attctaagat gaatttcttt tgctcctgct
    21241 tctcttaaag attttactaa ttgtttagat gtagttcctc ttacaattga gtcgtctact
    21301 aaaattatag attttccttt tattattgtt gaaagaggat ttaattttat ttttactgct
    21361 atttctcttt cttcttgtgt aggttttata aatgttcttc ctacatatct atttttaact
    21421 aagccttcac ttatttttaa tccactagca tttgcataac caatagctcc tggccaacct
    21481 gaatctggaa ctggaactac catatctgcc tttacagttt cattctgagc taatctctca
    21541 ccacatttaa ttctaaattc atatgcattt acattgtcta tagttgcatc atttcttgca
    21601 aagtatatgt gttcaaatat acaacttttc ttaactggtt tataattttc tgaataaaaa
    21661 tatgatttta gttctccatc tttgactact attatctctc ctggctctac atctcttata
    21721 acttctccgc caagtatatc tattgcacaa ttttctgatg caaatatata ttcatcaccc
    21781 tttttaccaa gtacaagagg tctgaatcca tgtgggtctc taactgcaac caattcttct
    21841 tgactcatta ttacaagagc atatgcacct tttatataat ccattgtcac ttttaaactt
    21901 tctactatat cccctttgta atatctagca agtatatata aaatcacttc tgtatctgaa
    21961 ttagtttgga acatatatcc atcttcttct agcatatctt ttaaataatt tgcattaact
    22021 agatttccat tatgagctat ggctaattgt ctttttctac aacttccaac taaaggttga
    22081 cagttagaaa catgacttcc tccagctgtt gaatatctaa catgtccaat ccctatattt
    22141 ccttttaact tagataattc attttcctta aaaacatctc caactaatcc catatctttt
    22201 ttgtacttaa tttctccatc atctaaaaga gctaaaccac agctttcttg ccccctgtgt
    22261 tgcatagaat ataaagaata atatagttct ttagttacat ctttattaga gtaaatccct
    22321 aaaactccac acatacattt gcctcctatt tattattcat tcttgaaagt acttccgtat
    22381 aaccttctac aaggtctcca aggtcttttc taaatctgtc cttatctaac ttttcatttg
    22441 tatttacatc ccaaagtcta caagtgtctg gtgatacttc atctgccaat attatatttc
    22501 cttcagaatc cttaccaaat tctattttaa agtcaactaa ctttaggttt aattttaaga
    22561 aaaatgcttt tagtaactcg tttattttta aagtttcttc tcttaagaat tttaactctt
    22621 ctctagttgc taacttcatt gccactgcat aatcatcatt tagcattggg tcaccataag
    22681 catcattttt gtaacttatt tcaaaaattg gctcatcgaa aactacacct tcttcaagac
    22741 caactctttt acagatagaa ccagcagcaa tatttcttac tattacttct aatggtaaaa
    22801 tctctacttt tttaactaac atttctctat ctgataaact ttttataaag tgagtattta
    22861 tattattttc ttttaacatt tcaaagatta ttgttgatat cttattattt agtatacctt
    22921 ttgaagatat ttctgctttt ttttctccat taaatgctgt tgcatcatct ttataataaa
    22981 caacatattc attttcatta tctgtagaat atacttgttt tgcttttcct tcatataata
    23041 acatttctgc taccccctta aattcttgtc gtcttctaac accttttgtg ccatctcttc
    23101 tctatatgat ttcaaatcct cttttaattt tgggtatttt aaagtcatta tttgtaatgc
    23161 caataaagct gcgtttaaac ctaagtctat tgtaactgtt gcaactggga ttccttttgg
    23221 catttgaact attgataaaa gtgagtctag tccatccata gttgatgatt taataggtaa
    23281 tcctattaca ggaattaatg tttgtgaagc tattacacct ggtaagtgtg ctgcttttcc
    23341 tgctgctgct attattacat cagtatcatc ttctatttct tttaggaata ttgaaagttg
    23401 ttctggtgtt ctatgagctg ataatgctct tgctacaacc tctattccat atttttcaag
    23461 cagttttatc ccttcttcta attttggata atctgatttt gaacccataa ctactgctac
    23521 tttcattaga cttcccctct ccgtttataa gttattttac actacaaatc attcgtatta
    23581 ttatacgaat tatacacctt aaattttata ttaatataca tatattatca actctttttt
    23641 gaaatttttt aaaaaaaata tttcttgtat ttttttcatt cgctacgtat cgaactttat
    23701 ttatgttttt ttgttaaaat aaaaaacaga ttatctcaaa attggttttc taattaaatt
    23761 ttaagctcaa tactcttttt taaatcttgt atattatttt ttattacttc ttttttaatt
    23821 aatatcttgg atttatagtc ttgtttttta gttctgaatg ttacttttac ttagataaaa
    23881 ccaacacaaa gtttattctt tatataagta atagctaaaa cttaaagtaa tgatattata
    23941 taaatagaaa taattttatt taaaggagaa tctgtatgtt agaagttata aaaaatagac
    24001 gttcaataag aacatatata gataaaaata tagaagaaga taaaataact gaaatactaa
    24061 aatcagctat gcaagcacct tcatcaaaaa atgctcaacc ttgggaattt attatagtag
    24121 atgataagga actgctgaaa gagctctcaa agtcacagca tagagcaaag catatagaat
    24181 ttgcaccatt atgtatagta gtgcttggaa atagagataa attcttaaaa cctggtaaat
    24241 ggatacaaga tttaggtgct tgcacacaaa atttattatt agaagttact aatcaaggtc
    24301 tagctgcttg ttgggcagga gtatttccta aaaataaagt agtaaataaa gttaaacaaa
    24361 ttttagactt accagtaaaa ttagttcctt atgcacttat atctattgga tatagtgaag
    24421 aaaaaaatga atttatagac cgatttgatg aaaataaaat acatagaaat gtatataaaa
    24481 atagataatg tttagtaata taaatgaaaa tacatagtaa a
//
LOCUS       DAEPLF010000036        24488 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEPLF010000036 DAEPLF010000000
VERSION     DAEPLF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24488
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.036"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            118..1191
                     /locus_tag="KR314_001305"
     CDS             118..1191
                     /locus_tag="KR314_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBH0327697.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            1261..1524
                     /locus_tag="KR314_001306"
     CDS             1261..1524
                     /locus_tag="KR314_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327698.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            1730..2707
                     /gene="bioB"
                     /locus_tag="KR314_001307"
     CDS             1730..2707
                     /gene="bioB"
                     /locus_tag="KR314_001307"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBH0327699.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMVFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            2935..3948
                     /locus_tag="KR314_001308"
     CDS             2935..3948
                     /locus_tag="KR314_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0327700.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNSAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDVSVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            3990..4880
                     /gene="rbsK"
                     /locus_tag="KR314_001309"
     CDS             3990..4880
                     /gene="rbsK"
                     /locus_tag="KR314_001309"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBH0327701.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIKVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKKLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            4928..5890
                     /locus_tag="KR314_001310"
     CDS             4928..5890
                     /locus_tag="KR314_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBH0327702.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            5944..7455
                     /locus_tag="KR314_001311"
     CDS             5944..7455
                     /locus_tag="KR314_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBH0327703.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            7439..8437
                     /locus_tag="KR314_001312"
     CDS             7439..8437
                     /locus_tag="KR314_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBH0327704.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            8621..9883
                     /locus_tag="KR314_001313"
     CDS             8621..9883
                     /locus_tag="KR314_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0327705.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFQMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            9903..10646
                     /locus_tag="KR314_001314"
     CDS             9903..10646
                     /locus_tag="KR314_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBH0327706.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            10819..12204
                     /locus_tag="KR314_001315"
     CDS             10819..12204
                     /locus_tag="KR314_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0327707.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFEYTPII"
     gene            complement(12541..13185)
                     /locus_tag="KR314_001316"
     CDS             complement(12541..13185)
                     /locus_tag="KR314_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0327708.1"
                     /translation="MNNLDKNNVNEVLDMLLRTRRSIRSFTKEVPSKGLIEEIIETGR
                     LAPYAILSVAKQTDFRHFFVISNNSLQMNKIKTILVDVIKTQISVNEKKAKNNITLQS
                     FITSLKMQVEKGVLGIGTAPYLIIVAERRGIPPIEQKSLSHVMQNMWLKSTALGLGFE
                     LVSVIGRLSDNKDFCDILGVPTGEYGFDACAIGFPTTSVTREGRAIPHPSIKWI"
     gene            13449..14063
                     /locus_tag="KR314_001317"
     CDS             13449..14063
                     /locus_tag="KR314_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBH0327709.1"
                     /translation="MKKVSIDRRTIRTKKMIRSALAELIDEKGFNNISVTDLTQRADI
                     NRGTFYLHYVDKYDLLERIENEIIQEIEEETKHLDSSNMMEIDTSSEPLPFMVKLFEY
                     FRKNSVIIKSILGPKGDPTFERKIKKFIETNLFEKQKLINFNIDEAIISEEYFIQYIL
                     SADLGVIQYWLEKNMKESPEEMALILARMSLLGPLRAVGIRKDS"
     gene            14284..14424
                     /locus_tag="KR314_001318"
     CDS             14284..14424
                     /locus_tag="KR314_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327710.1"
                     /translation="MKNENISYLALGLCFGVVLGSLFKNLAIGLSFGLVIGVVLDSKK
                     NK"
     gene            complement(14654..15101)
                     /locus_tag="KR314_001319"
                     /pseudo
     CDS             complement(14654..15101)
                     /locus_tag="KR314_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599264.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            15871..16710
                     /locus_tag="KR314_001320"
     CDS             15871..16710
                     /locus_tag="KR314_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327711.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            16700..17143
                     /locus_tag="KR314_001321"
     CDS             16700..17143
                     /locus_tag="KR314_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327712.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            17154..17645
                     /locus_tag="KR314_001322"
     CDS             17154..17645
                     /locus_tag="KR314_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327713.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(17847..18575)
                     /locus_tag="KR314_001323"
     CDS             complement(17847..18575)
                     /locus_tag="KR314_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBH0327714.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            18700..19788
                     /locus_tag="KR314_001324"
     CDS             18700..19788
                     /locus_tag="KR314_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327715.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLIIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKELMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            complement(20226..21074)
                     /locus_tag="KR314_001325"
     CDS             complement(20226..21074)
                     /locus_tag="KR314_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327716.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMSNNMNSNMGMPNNMPNNMNSNM
                     GMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            21273..21638
                     /locus_tag="KR314_001326"
     CDS             21273..21638
                     /locus_tag="KR314_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0327717.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            21661..24048
                     /gene="cadA"
                     /locus_tag="KR314_001327"
     CDS             21661..24048
                     /gene="cadA"
                     /locus_tag="KR314_001327"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBH0327718.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLIVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENASSKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFKEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELVPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGESSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKFNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKAIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
BASE COUNT         9608 a         2539 c         4335 g         8006 t
ORIGIN      
        1 aataatattg ttttcattaa ataaagataa gaatctttaa ttgtggtaaa atatactaaa
       61 aaatcatttt aaaagtgata aaattatttt aagtaaatat tttaaagggg tgtagatatg
      121 gaaaagtcaa aagtatattt ttgtgattta tactcaaatt cgcaaaataa aaacgtacca
      181 aacaatgtaa gaagattatt tgatgaagca ggattcaaag atttaataga aaagaatgac
      241 caagtcgcaa ttaaacttca ctttggagaa aagggaaata ctacatatat gagtcctgtg
      301 gcagttagac aagtggttga taaggttaaa gactgcgaag gaaagccatt tttaacagat
      361 acaaatacac tgtatacagg gagtagaaca aattctgtag accatcttac aactgctata
      421 gaaaatggat ttgcttatgc agttgtaaat gcaccagtaa taattgcaga tggattatat
      481 agtagaaatt atgagaatgt aaaaatagac aaaaaacatt ttgaaagtgt aaaaataggt
      541 ggagaaattt ataattcttc tgcaatgata gtaatgagtc attttaaagg tcatgaagca
      601 gcaggttttg gaggagcatt aaaaaactta gctatgggat gtgcaagtgc agcaggaaaa
      661 caaatgcaac attctgatgt tactccagta gtaaaggaaa agaagtgtgt aggttgtgga
      721 aagtgtgtta attcttgtcc tacaaaagct ataagtatag tggataaaaa agcagttata
      781 gattctgatg tatgttatgg atgtggagaa tgtccaactg tctgtccaac tagagcagta
      841 actatacaat gggaaagtga ctcagatgtg tttgtcgaaa agatggcaga atacgcttat
      901 ggagcagtat caaataaaaa agacaaggta gggtatataa cttttgttat gaatgtaact
      961 ccactttgtg attgtgtacc ttggtcaggt agaccaattg cccatgatat aggaatatta
     1021 gcatcaacag accctgtagc tattgaacaa gcatgctatg atttgatttg taaggaaatg
     1081 ggtcatgatg ttttcaaaca tgaacatcct catgtaaatg gaactagaat aatagactat
     1141 gcatgtgaaa tgggtatggg aagtaaagaa tatgaattaa taaaattata agaatagtag
     1201 cacttaaatg catataatta ttgtaagaga aatctaaaag tatcaaagga gtatgcattt
     1261 atgaaaattt actatgagaa ggaaaacagc aagaaggcta atgtaaacaa tattccaagt
     1321 gtatttgcgt ttaattttct aagaaacttt ttgactagca aagagtatga ggaaactaga
     1381 aaagaatatt ttataaataa tctaacaaaa agtcaggtta atcaagccat gaaggattta
     1441 aaatggctat ttagagaata tgaaggctta gaggttatta caatggaaag cttggatgga
     1501 gttaagacaa ggatagtttt ataaaccagt aagaattata ttctaataaa aaatactgct
     1561 ttaataataa tatgtataat ctatatacta atattatttt gaagcagtat ttttttgtgt
     1621 atagtaaact tgtgtctaaa ataaagttga ccaataaatg ttatatatgc aatactatat
     1681 ataataagta aactacataa aaatattaag ttgacaaata ggaggatata tggaaaaata
     1741 cataataaaa ttaaaaaata aagtattgag agaaaaagaa ataagttatg aagaagctct
     1801 taatttaata agccttgata caaataataa aaacgatttt gatattctat taaaatctgc
     1861 aaatgaaata agagaatatt ttatgggaag aaaagcagat ttatgtacga taatgaatgc
     1921 taaatctggc aaatgtagtg aagactgtaa attttgtgct caatcatctc attataaaac
     1981 tggtgtagaa gaatattcat tattagatta caatgaaatc ttaaatagag ctaaagagat
     2041 ggagagtaaa ggagtgcata gattctcatt ggttacaagt ggtaaaggta tgtcaggaaa
     2101 ggagtttaat aatattttaa atatctatga aggattgagg gaaaatacta atttaaaatt
     2161 atgtgcatct ttaggtatta tagattatga gcaagctaaa atgttgaagt cagcaggtgt
     2221 gactacatat caccacaatg ttgagacgtg tagagataat tttcataata tatgtacaac
     2281 acacacatat aaagatagga taaaaactat aaaagatgct aaaaaagctg ggctagatgt
     2341 atgtgtaggc ggtataattg gaatgaatga gagtgaagaa cagagactaa aaatggtttt
     2401 tgaaatacga gaattaaatg taaaatcatt tccaataaac attttaaatc ctattaagaa
     2461 tacgccaatg gaaaattatg atgtattaga accaatggaa atactaaaaa caacagcagt
     2521 atttaggttt ataattccta atgtgtacat aaggtatgct ggtggaaggc tatcattaaa
     2581 gggatatgac aaagttggat ttaatggggg agtaaattct gcaattgttg gtgattattt
     2641 gacaactgtt ggaagtggga ttgaaaatga taaaaaaatg attatagagc aaggatttga
     2701 actttaataa atactactta aattaattaa tatactttta gataattata aaagtatatt
     2761 aatttttttt gcagattaat ttaaatatta aaattgaatt ataagttgca atattttgaa
     2821 aaaagggaaa aatatcaaaa tttatataaa atggttgcaa atgggaaaag gatgtgatag
     2881 tatctaggta accggttaag taaaacggtt tcctaaattt tgggggtgaa cttcttgagt
     2941 ataactataa aagaaattgg cgaattggct ggtgtttcta aaacaactgt gtcaaaagtc
     3001 attaataata aagatgaaaa tataagtcag gcaacaagag aaaaaatttt gaaaataatg
     3061 aaggaaaaaa attatgtacc aaataaatta gcacaaagtt tagtaactaa aaaaactaat
     3121 actatagggc ttcttatacc agatatacga aatccattct ttacagatgt atcaagaggt
     3181 gtagaagaca aagctaatga agaaggctac aatataattt tatgtaatac agatgaagat
     3241 gctaaaaaag aatatgaagg aataagaaca ctatcagaga ggatgataga tggaattata
     3301 tttgcagcat cttcaaatac aaactggaaa gaagcaaatt ataaagatat aaaaatacca
     3361 actgtcttga ttgataagaa gataagtatg aacaaagaaa ccttaaaagg aattgttaaa
     3421 ataaataact ttgaaggggc ctatatagct acaaaacatt tattagatat tggaaataag
     3481 aaaataatat acctaagtgg tccacttcaa aatgaaatag cagtagatag attagaagga
     3541 tataaaaagg ctctaatcga gtgtaattta agctacaatt ctgcttatgt atttgaagga
     3601 aaatataaga ttgaatgggg acaagaattt ataaaaaatt tagaaaagat agattttgat
     3661 gcaatatttt gtgcaaatga tttgattgca ataggtgtca taagaggtct taaagaaaga
     3721 gggttaagta taccaaatga tgtaagtgtc gtgggatttg atgatattca aacatcaagt
     3781 ctaataagcc cttctcttac cacagtaaaa cagccgtctt atgatatagg atataaagcc
     3841 agtgaaatat tgataaattg tttaagagga gataaaaaag aatcatttga tgaattgatt
     3901 ttcaaaccag aattagtcat aagagattca acaaaagaaa gtgaataaca gcacgagaga
     3961 cataaataga tatgtaaagg agtaatgtaa tgggaaacat agttgtcata ggaagtgtaa
     4021 acatggacat ggtatgttct gtagataaaa gaccagaaaa aggagaaaca gtattaggta
     4081 atagtttttt tacatcacct ggagggaaag gggctaatca agctatctca gcttctaaac
     4141 taggagcaaa tgtaaaaatg atatcatgca taggggaaga tggcttagga gaggagttaa
     4201 taagaaattt tagaacgaat aaagttgatt atagtttagt atccagaaat aatcacaaaa
     4261 gttctggtgt tgctgttata acattatgtg aaaatgataa tagtattgtt gttgtaccag
     4321 ggacgaatga gctagtagat atagaattaa ttaaaaagaa tgaagaagag ataaagaatg
     4381 cagatatagt attgctacaa ttagagattc cattaaaaac aataaattat gtagtgaatt
     4441 tctgttttga aaataggatt aaggttttat taaatccagc accagcagta aaactaaatg
     4501 aagatataat agaaaaagta acttacttaa caccaaatga acatgaatat aagatagttt
     4561 ttgacacaaa tgaagggata gaagaagtat taaaaaaata tccaaataaa cttgtaataa
     4621 cagaaggaaa aaatggagct agattttatg atggtgagga aatcaagcat gtatcttgta
     4681 taagtgttga tgttcaagat accacaggag caggagatac atttaatgga gcattggcag
     4741 tggctataac agaagggaaa aaattatata cagcagtaga atatgcagta gtagtatcag
     4801 gtctttctgt aactaaacta ggtgcacaat ctggtatgcc ttataaagaa gatgttgaaa
     4861 aatatttaaa taataaataa aaatctaatt tgttcaaatt taaaactatt aaaaatggag
     4921 gttaattatg agagtgagaa aaattttatc tttaggaatt gctggaatac tagcaatagg
     4981 aatgttaaca gggtgttcaa tggaaggacc aagtaaatca gataataaag gtggttcaga
     5041 caagaaagat ttaactattg gagtatctac aataactctt caacatcaat tttttataga
     5101 tattgatgag ggaataaaag aaaaagctaa ggaattaggt gtgaaagtta tagtaaatga
     5161 cccagaccaa gatgtagcta agcaaacatc agcaatagaa gattttatac aacaaaatgt
     5221 agatggaatg atagtacttg gaactgataa ttcggctata gtaccagcag tagaaggagc
     5281 atttgaaaaa atgccagtag ttacagtaga tgctgtatta aatactgaaa atattacaag
     5341 ttatgtagga acagttagtt atgatgctgg taaaaaatta ggggaataca ctaaaaaata
     5401 catagatgaa aagttaggtg gaaagtcaga aatagcaata gtgacagatt taaaatctca
     5461 aatacaaatg cagagaatag atggatttaa agatgcgtta aaaggttcag caaatgttaa
     5521 gatattaaat tcacaaccag gatatgacag agaagaatct cttaatacag tagaaaactt
     5581 aattcaatct aatccagatg tagatataat atatgcaaca gctgaaaata gtgtactagg
     5641 agctaaagct gcacttgaat ctgcaaaaaa taaggatgtt aagattgtag gatttgactt
     5701 aactgaagaa gcatcaagtg gaataacaga tggaactatt cttgctatga ttcaacaaca
     5761 acctaaagag atgggacgtt tagctgtaga agcagtagta aaagctataa aaggggaaaa
     5821 ggtagaaaag aatataccag taccagcttt actttatgat aaggaaaata taaaagactt
     5881 taagaattag taaaataaaa tgagattatt tgaaagaaat cctatttaaa aaggagtgtc
     5941 attatgagta atataattct gaagttaagt aatattgcta aagaatttcc tggtgtcaga
     6001 gcattagata atgttaattt tgagttattt catggcgaag ttcatgcact actgggtgaa
     6061 aatggtgctg gtaaatcaac aatgataaag attttaactg gtgcacattc gaaaacttct
     6121 ggaaaattta tttttgaagg caaagaaata gagaatatat ccccagatat ctctaaaaag
     6181 ataggtataa atgccatata tcaagaacta acagtttttg acgaattaac agtagcacaa
     6241 aatattttta tgggaaaaga aataaatggc aaagtcttaa ccaatgacaa aaaaatgaat
     6301 gaagaggcta aaaagatttt cgataatatg ggaatagata taaatccaaa tagtttagtc
     6361 aaagagctta gtatagctca aaaacaaatg gtagaaatag caagagtttt atcatcagaa
     6421 actaaagtat taataatgga tgaacctact tcttcaataa gtaaaaaaga gactgaaata
     6481 ctttttagac ttataaatga tttaaaagag agtggagtta gtataatata tatatcccat
     6541 aggatggaag aactctttga gatttgtgac agaataacta taatgcgaga tgggaaaacg
     6601 atttcgactt tgaataccaa agatgtatcg tctgaggaag aattagttaa tttaatgata
     6661 gatagaaagc tagaccaatt tttcccaaaa agaaaagtag aaataaaaga agaaattatg
     6721 cgagtggaaa atctaactaa gaacaatgtt tttaatgata ttagctttaa tataagaaaa
     6781 ggtgaaatat tgggaatagg tggactagtt ggaagtaaga gaagtgaaat agtagaagct
     6841 atatttggac ttagaacata tgattctggg aaaatatatt taaacaatga agaagtaaag
     6901 tttaaaactc catcagatgc aatagaaaat ggactgggat taataacaga agatagaaaa
     6961 ggtactggat tgtttttaca aatgagtgtc aaagaaaaca caactatggc tgggttaaaa
     7021 aagatatcta agtttaaatc aattatagat agaaaaaagg aaaaagagat tttagaaaaa
     7081 tacatagaag ctttaaagat aaaaacacca catatgaatc aagttataca aagtttaagt
     7141 ggtggaaacc aacaaaaagc tataatagca agatggcttt tattacaacc agatatattg
     7201 attatggatg aaccaaccag aggtatagat gtaaatgcta aggcagagat atacaatcta
     7261 atgggagatt tagtggagag tggtgtaagt ataataatga tatcttctga aataccagaa
     7321 ttaatatcaa tgagtgacag aataatggtt atgagagaag gtcatataag tggattttta
     7381 gaaggtgaag aaatggttga aaataatgta ttaaaattgg ctttcggagg taaaattaat
     7441 gagttcaata actaagcata aagaagaaaa atcttctcca ttattgacaa aattaacaag
     7501 tgtatttaga gaccaaggga gtgcagctgt aggacttgta ataattttta taataatgag
     7561 tgtagcttca tctaactttt taactttaga taatttaatt aatgttggaa gacaaatatc
     7621 aataaatgca atacttgcag taggtatgac atttgtaata attacaggag gaatagattt
     7681 aagtgtagga gcagtaattg ctttagtagg tacattttgg gctacaacag tagtgaatta
     7741 taatgcacct atatgggtag gtatgatact tgcattagca atagggacaa tattaggagt
     7801 cataaaaggt gcaattattt caacacaaaa actacctcca ttcattgtaa ctttagccat
     7861 gttaacgata ataagtgggg caagctttgt atttacagga ggaagaccta tatcagtaaa
     7921 tacagatgca tttaagatgc tcggtagagg atatattgga ccaataccaa taccagttat
     7981 aattatgatt atagttgtaa tagcaggaca ttttctatta aaaagaacta attttggacg
     8041 tcatgtacat gcagtaggtg gaaatgaaga ggccgctaga ttatgtggag taaaagtaaa
     8101 taaagttata gtaaaagtat acgcactagc tggactacta acagcattag ctgggataat
     8161 actatcatca cgtttggcat caggttcacc aactgttgga gatggagcag aattggatgc
     8221 tatagcagcc gttgtgcttg gtggtactaa tatgatgggt ggttctggtt ctatagtagg
     8281 tacatgtata ggtgtcggaa taataggaat attaggaaat ggattaaact tattaagtgt
     8341 atcttcatat aatcaaatga ttataaaagg tttggtaatg ttatttgcta tatggataaa
     8401 taatataaaa ttaaaaaaat cagctaaatc taaatagtaa ttaaatttta gcttataaac
     8461 tataaaaatt attaagtttt tgtatatatt tttatacttt aatttgcata catctttata
     8521 cattgaatgg gaatatgcta agtgaaaaag tttactatgg cttaaataga tgagattaga
     8581 cttgaaattt taaatgtaaa aataaatctt aggaggaaat atgaaaaaaa ttctcttaga
     8641 tacattaaat agtaaaaaac aagaatacat agattattta aaagaacttg taagtataaa
     8701 gactgaagat gttggtcatg gaatactagg tggatttgaa aaagaaggac aggaatatat
     8761 tgaaaaactt gcaaattaca taggttttag tgtagataga caagaaatga gcgaagaatt
     8821 aattaaaaaa gcaaaaaata tatataaaga aggaaatcta ggtcacaatt atcaagatag
     8881 atataattta atttgtaagt atagtgatga tttacctggt aagacaattg tatttaatgg
     8941 tcatgtagac actatgccac caggtgatat aagtaagtgg aaatataatc catatcgtgc
     9001 tacagaagat aatggaaaac tttatggtct tggaactgct gatatgaaat ctggacttat
     9061 agcatcaata ttagctgtaa agcttataaa agatagtggt ctaaatgtac ctggtaatgt
     9121 aaaaataatg tcagtagtag atgaagaagg tggcggaaat ggtaccataa atgcagttat
     9181 gaatggtatt ggtggagatt gttgcataat ttgtgaacca tcagaacaaa acttaatagt
     9241 tgcacatatg ggatttgtat tctttgaagt tgaggttaaa ggtgtgtctc ttcactgtgg
     9301 tagtaagtgg gagggtgtaa atgcaattga gaaggctatg ttgttgcttc aagatataaa
     9361 agagttggaa cataactggc ttatgatata taagcatcca ctattacctt cacctacaat
     9421 aaatttaggt gtaataaatg gtggaacagc aggttctact gtgccagata agtgtgtatt
     9481 taatttatgt gtacacttct tgccaaatat aatgagttat gaacaagttg taaatgatgt
     9541 aacaaatgtc ataatgacaa gggctaatgg agatttatgg cttaaagata ataaaccaaa
     9601 tataaacata tatcaatcag ggttaggttt tcagatggac aaggattctg attttgtagt
     9661 gaatgcacat aaaatactag aagaaacttt aggaaaaaaa ttagagataa aaggttcaac
     9721 agctggcaat gatgcgagat taatgaaaaa tttagctgaa ataccaacac ttatattagg
     9781 gccaggttca atagaacaat gtcattcaat tgatgaatat gttgaaatta aggagtattt
     9841 agatagtata cttatgtatg caagtctaat attaaactta taaaagtagg aggaatatat
     9901 aaatgaaaaa agtattaata gtaggggaat cttgggtaaa aaatataact catataaaag
     9961 gatttgatac gtttgttaca actcattatg aagaagctgt aaagtggttg aaagaagcaa
    10021 tagaatctgg aggatatgag acagttcata tgcctgcaca tgtagcagca gatagttttc
    10081 cgtataaatt agaagagtta aatgagtatg actgtataat attatctgat ataggttcta
    10141 atacgttctt attatctaat agtactttta tagattgcaa tagtaatcca gatagacttg
    10201 aactaatcaa agagtatgta aataatggtg gggcgttaat catggtaggt ggatatatgt
    10261 catttacagg aatagatgca aaagctagat ttggagaaac tgctatcaaa gatgtattac
    10321 caataacaat gatagataaa gatgatagag ttgaaaaacc agcaggaata attccagaag
    10381 ttattgactc tgaacatcca gtattaaaag gtataccaac agaatggcca aagtttttag
    10441 gatataacaa aacagtagct agagataatt gtcctgtttt agcaactatt ggaggagacc
    10501 catttgtagc tgttggcgag tttggaaaag gaaaatctgc tatctttagt tctgactgtg
    10561 cacctcactg gggtccaaaa gaatttactg attggaaata ctataataaa ttatgggtta
    10621 atatgcttga ttggttaaca tgctaaaaat tttataaaac ttgaaaacct cttacttgat
    10681 aaaatgaaac ttacaagtca agaggtttta ttgtataaaa tggttgtatg gtagaaaata
    10741 tttttaaatc agttaaaatt agtcattttg gctgataatc gataagttat tagaaacagg
    10801 gaggtttata taattaatat ggataattta aataaaaagt ttttgatgga atatatagat
    10861 gagatacagg gagaattaag agatatatca gataagatat ggaaaaatcc agaacttcag
    10921 tataaagaat actatgcttc aaatttacaa aaagaatgtt taaaaaaaca tggttttagt
    10981 atagagggat tagaaggtat ggatacagca tttgtggcta gttatggaga aggtagacca
    11041 gttatagcag ttttgggaga atatgatgct ttagatggtt tgtctcaaaa agtatccact
    11101 actaaagaac ctattgtaga tggtggtgca ggtcatggat gtggacataa cttacttggt
    11161 acaggttcaa taggagctgt aattgcagta aaggaattaa tcaaactaaa aaagctgaaa
    11221 gggactataa aatactatgg atgtccagca gaagaagatt tatctggaaa agtattgatg
    11281 ataaaaaaag gtttttttga tggaattgat tgtgctttta gttggcatcc atttgatata
    11341 aatactccga taagaatccc aacacttgct aattactctg tgaaatttag atataatggg
    11401 attagtgctc atgcagcaca agctccttat aacggaagaa gtgcacttga tgcagtagaa
    11461 cttatgaata taggttgtaa ttatttgaga gaacatatat ttgataattg tagagtacac
    11521 tatgtaacta ctaatggtgg aaaaatgcca aatatagttc ctgattttgc agaagtgtgg
    11581 tattacataa gaggagttaa aatggagcat gtaagagatg tatttggtag gattgttgat
    11641 atagcaaaag gagcagcatt aatgactggt actattatgg aatatgacat tataagtgga
    11701 gtatatgact atattccaaa tacaatatta acagatattc tatcacaaaa tatgaaatta
    11761 gtaggtgtgc aaaactataa tgaagatgac tactattttg cagataaatt agctgagaca
    11821 gtgtctatag ataaaagaag tagtgtttct agtgtattaa gtggaaataa ggatattact
    11881 aagatgaatt tacatgatga ggttacagat gatacattta ctcataataa ctgtatatca
    11941 ttatcattgg atattggaga tgttagctac ataattccta cagcacaatg ttcttgttca
    12001 gtttggccta ttggtatttc tgcccataca tggcaatctt gtgccagtgc aggttctgat
    12061 atgggattta aggcaatgtt attggcatct aaatctatat catgttcaat aaatgatgta
    12121 atgcttgatg aattggttat aaataaagca aaaaaagaat tgaaggatac tgtaggtagt
    12181 tttgaatata ctccaattat ttgaagagta atttaataca agtaagattg aattgtatga
    12241 attaggatga atatataaat taatattatt tttaacttaa ctttctaatt attttcaatc
    12301 taagatacaa taattagaat atatttttaa agtttcctat ttaaggaaaa taatttgatt
    12361 ttatgtttga taaaaggact tataatttgt aagccctttt attatattta ttttttgttt
    12421 ataattaatt ttatttttta ggtttagtta ggttatagat taccctgaaa aataacattt
    12481 aacttattat taaaaacatg taagaatagt ttataaatat tttattttcg atgttgttta
    12541 ttatatccat tttatcgatg gatgaggaat ggctctgcct tccctagtta cagatgtagt
    12601 agggaaacca attgcacagg catcaaatcc atattcacct gttggtactc ctaaaatatc
    12661 acaaaaatct ttattatcgc tcaatcttcc tattacacta acaagttcaa aacctaatcc
    12721 taaagcagtt gatttaagcc acatattctg catcacatga gataatgact tttgttctat
    12781 aggtggtatt ccacgacgtt ctgccacaat aataagataa ggtgctgttc ctataccaag
    12841 aacccctttt tcaacttgca tttttaagga agtaatgaag gattgaaggg taatgttgtt
    12901 ctttgccttc ttctcattga ctgatatttg agttttaata acatcaacaa gaatggtttt
    12961 tattttattc atttgtaaac tgttatttga aataacaaag aaatgtctga aatctgtttg
    13021 ttttgctact gaaagtattg cataaggtgc aagtcttcca gtttcaatta tttcttcaat
    13081 cagccctttt gaaggtactt cttttgtaaa acttcttatg gatcttctag ttcttaatag
    13141 catatccaaa acttcattaa cattgttttt atctaaattg ttcattttta tcctccacta
    13201 atagtattct taagatgttc tataatgcat ccatataaag gcgaactttt cggtcatatt
    13261 aaacatttcc ttcaaattaa gtcgctattt ctatgaacac aatgttgatt aataatctat
    13321 atgtatatta taatatttat attgcattat gcaataaaca ataatattta aactgttaag
    13381 taataaacat ttactataaa attgttaatt aaaattaaaa aaatttatat ggaatgagga
    13441 aataattcat gaaaaaagta tctattgata gaagaactat acgtactaaa aaaatgatta
    13501 ggtctgcatt agcagagtta atagatgaga aaggatttaa caatatatca gttactgatt
    13561 taactcaaag ggcagatata aatagaggaa ccttctatct gcattatgtt gataagtatg
    13621 acttgttaga gaggattgaa aatgaaataa tacaggaaat tgaagaggag accaaacatc
    13681 ttgattcatc caacatgatg gagatagata cctcaagtga acctcttcca tttatggtaa
    13741 agctttttga atattttaga aaaaattcag taattattaa atctatcttg ggtcccaaag
    13801 gtgaccctac ttttgaaaga aaaataaaaa aatttattga aaccaatttg tttgagaaac
    13861 aaaaactaat aaattttaac atagatgagg caataatttc agaagaatat tttatacaat
    13921 atatactttc agcagactta ggtgtaattc aatattggct tgaaaaaaat atgaaagagt
    13981 cccctgaaga aatggcactg atacttgcta gaatgtcgtt actcggtccg ttaagagcag
    14041 tgggaattcg taaagattca taaaaatagt tttatatata ttagactttg aaaatatatg
    14101 tttaaaaacc attttttact actactgttt ataaattagg ggagatatct taaaatatta
    14161 gtaaaaggag tgctaagtga gtgtaataac tatgagataa gaggtttagt tgatatagtc
    14221 aaaacttcaa aagaatcaat tagaaaagtt aaaatattgc aattaaattt aaaggagtaa
    14281 tatatgaaaa atgaaaatat aagttatttg gcgttaggat tatgttttgg tgttgttttg
    14341 ggttcattat ttaaaaactt agctattggt ttatcctttg gattagttat tggagtagta
    14401 ttagacagta agaaaaataa ataaaacttc aatagtaaca tataacaatt atactatttt
    14461 tgtataaaat cattggaaat caacaataga aaatatttgt tatttaaaaa ctaaaaatac
    14521 agtttaaacg acctaaaaat atattatata attattataa caagataagt gagagtaagg
    14581 ttatgataat agagtaatat ttttcaatta ttactctatt tatatttatt tagttatact
    14641 atagtttata ttgttatttt attttcatat gcagtatagg gaatggatta ccttgttcat
    14701 cgaactcaga ttttttaaag actttaaatc ccatatgttt ataaaagcct aatgcttgtt
    14761 gattttgctc attcacatct acataattta catttaatac ttctattgca tattctacaa
    14821 gtctttttcc aattccattt cctatactat aattacttac aaataacatt tcaattttaa
    14881 aatcatgtat tcccataaaa gcttttataa tacctttttt tgtctcttac acataaaaaa
    14941 ttactaacat atttagcaca ttctttaact tgaggcttta ttgatattat attttcttca
    15001 cttaaaaagt catgtgttgc tcttactgat gattcccgtg tatctagtat ttctttcata
    15061 tctttattgt taataatatt taaattttca atttttatca tttaataatt cctcttaagt
    15121 aagtatgtta cttaatatta tatgttaaat atgatttaaa aaggttataa gaatattttt
    15181 gtattaaata taatcaaata aatatattta tttgtcataa attaaatgtc cactaaaata
    15241 gaaaaaataa gaagaaatag gtaataaatg atagcttata gcaaaataaa gatgtaagta
    15301 taacaataga tgagattgga attaagtcac tatacctaac agaacattct taggcgaata
    15361 tagaagactt aaaaaagaaa agagaagtag acaagaattt attaatgatt acatataaaa
    15421 taaatgaatt ataattttat taatgatata ataataatta acaaaaaata aaagaggaac
    15481 gagttaatgt ttgatggcat acactcattt tctaaccaat aaaacaatag attcaattaa
    15541 ggttactaaa gtctaatcaa cacataaaca tataggataa atacatcttt ttaatatatt
    15601 aattatgtta gacttacaaa aaatgtaaat acatagcttg ttaaatcttg attgaacctt
    15661 aagtaataag ggataaaaaa tgacttgttc aaaggcaaag aatatactat taaatgatat
    15721 aagtaactta gagataatat gtagtaagtc aaaagaacta tatagcatca gtaagtaaaa
    15781 agcgtaaaaa cttacataat gcactataaa ataaatgagt tagaaaataa atataattta
    15841 tataataaag tagtacaaaa aggagtattt atgtttggat atgttaaaat aaacaaaatg
    15901 gatttaacat ttagagaata tgattattac aaagcttact attgtggact atgtaaatac
    15961 ttaaaaagaa atcatggaga aatctctagg ttttctttga attatgatat aacattttta
    16021 attgttttat taacagcagt atataaccca gagtctataa gtacagagga agtgtgtata
    16081 gtaaatccat ttaaaaagaa aaaggtaatt acaaatgata ttacagaata tgcagcaagt
    16141 atgaatattc ttttaacgta ttataaattg gaagataatt taatggatga taaacgcata
    16201 aaagataaat tggcatatta tatatataaa aacaaattaa aattagcata tgaaaagtat
    16261 cctgaaaaag cagagtatat aaaacaacaa ctaaatgaat taaataagtt agaaaaagat
    16321 aaaaatataa atatagatga agtatctagt atatttggaa atataatggg tgaagtattt
    16381 gtctataaaa aagatgaaaa tgagagaaac ttaagaatga taggttttaa tataggtaag
    16441 tatatatatc tactagatgc ctatgaagat ttagatgaag attttaagaa aggaagatat
    16501 aatccattta tagagtatat tgataaaaat gatgagttaa aagaaaaagt aaaaaaaata
    16561 atagtaacat cattaggatt tttagctagg ggaattgata atctaaatct aaataaaaat
    16621 gttggaataa tagaaaatat aatttattct ggtgtttatt taagatatat taatatatta
    16681 gaaagtagag gtggaaagaa tgtacagtaa tacttataat aatgaaaatt ttatgagagc
    16741 aagagcattc atagaaaata gtgaatttaa aaaagcatat gattttttaa aaactttgac
    16801 tgacaaatgt gcagagtggt attatttaac tggcttttct gcaatgaaca taggatatta
    16861 tgaagaaggt gaagacttct taaaaagagc aaaatttatg gagcctgaaa atagtgaata
    16921 tagtgatgca cttagaagtt atacacaata tagaaatgat tatagcaata gggctgacaa
    16981 ttataataga agaagacgta atgatttaga tggatgttgt tgctgttgct gtgatgattg
    17041 ctgttgttgt ctaggtgacg attgttgtga aaattgtgct aagctttggt gtttagattc
    17101 atgttgtgaa tgttttggtg gagatttaat aacttgttgt taggatgata aatatgagta
    17161 taaaagttgc ttatggagga gttttattag cattaaatgt gatattgttg actcttacaa
    17221 atattattcc tgtaaataca ttgtttatta tggggttagc atcactttta gtatcaattg
    17281 taataatgga atggggcttt aagagtggaa tcgcttttta tataggttct atagttttag
    17341 gatttatagt gatggcaagt aagtctcaat ggattgtata ttcacttacc tttggaatat
    17401 atggtattgt aaaatatctt atagaaaaag ataggtctat atatattgag tattttatga
    17461 aattagtatt tgcaaatata atgatactta tattgtattt tttattaaga acaatagttt
    17521 atattccaat aaacatattt attataggtt catttgagat agcctttata gtatatgact
    17581 atgtttacag ttcttttata ggttattaca ataataggtt aagaaagatg ctatttaaaa
    17641 aataatagtt gatttttata tttaatttta atgaaattgt agtttggata agtgtaaatg
    17701 atatatctga atatacatta aagcataaag ctaaatttaa tatattctta gtgcaacata
    17761 taaaagcttt gatttttagt caaagctttt atatgttaga tataaatatt agatataata
    17821 taagttttta aaatagatag tataaattaa atatccacta tatttacatc tgtagataga
    17881 gaataaataa tatcttttct ttctactatt atctcaacat ttttagaaat ttcctcatca
    17941 agtgatattt gagataaaag ttcctttgtt ggatttattg ctattggatt tccaactctt
    18001 tttagcatat ttatatcacc attagtatct ccataggcat acgagtttga taaatctaaa
    18061 tcatacttta caacaaaatc atttatagca ttattcttac ttatagaatc ccacattgga
    18121 ataactgtgc ctgtaaatat attattttca aagacataat cacttcctag aaaatcggtt
    18181 acattatatt tttttgccat tttttcaaca agaaatccag ggctaccaga tataaatata
    18241 actatgtgtc cttgatttag atgccactca atccttgaac gtgtgtattt ataaactcta
    18301 tctgctttta atttaattac ttggtcactt gtaaagttga tacaggtttt atctaatcct
    18361 tttagtgaat caacataaag gtcacatatt tctaatagat aatcatcata attaccttgt
    18421 cttttatccc aatccataaa agtgtctcta gcatgtttat accaattttt ttggtcaatt
    18481 atatcgtatt taattaattt tttaaaatgt tcaaccataa gagagtctct ataaagagta
    18541 ccatctatat caaaaaaagc tgctatattt ttcataagac cacctcaatt aatcatttcc
    18601 ttatattata ttataaattg tatctaagtt gttaaaatat atgtataata atttatatat
    18661 taaaaaacaa gtattttaga gtttaaagga tgtgactata tgaattttaa ttttgcaaag
    18721 gctacaaatt ctagacttat gggcagttta ggtcttataa taaattggat agatgatgaa
    18781 aacaatcact tttgtcaata ttttttatta gatgcagagg gattaggatt agcagattat
    18841 gtgagtttaa ataacccaac acaagaagaa gcttatatgg aagaagaaag attgatggga
    18901 ggatttggtt cagatagagt tgaattaacg aaggatgaat ctttattttt agtatcttat
    18961 tttggtaata agaatcttta ttatgataag ctattgccag gagataaatg tgagtatatt
    19021 gatattataa aaaactataa aacagattta attatagaaa aattatacaa taaaatatgt
    19081 aaaagagttg atgaggaagt tgaatttata aactatatga ctatgagatt tatagcatgg
    19141 gatagagaaa gtttaaaata tttttctgga agtgacgaga ttgcaaatat gcatattact
    19201 aatataaatg gaacattact taaaaacgtg gtaagtgata aaggtcaagg cagatatata
    19261 agtgaagctt tatatgaaga caatgatgga tattatatat gtaaaattgc tttttgcata
    19321 agtaagtgct atgaaactgg ttttagaatt aattctctat tagtgacaga taaagaactt
    19381 atgtatgatt ttgaggtatt tgatgagatt tctaaaagtg aatttgtaag tgtttacagt
    19441 gtaaattcac ctgaagaatt tgcaggagta ttttatagag aaaatccatt tctgttaaaa
    19501 agtaatatga atgaaggtat attttttact agatttaatt ttaataatga ccatgtaaaa
    19561 gaaaatgtat atataataaa caatgatatg aaagctatat attacttaat gggaaataag
    19621 ttttttattg gtacttataa cgaaaatgat tgcaattata tcaatgaaat aagtcagtct
    19681 aattatagtg actatattaa atttgaagag aaatttttct ttgagcaaaa tgcattatat
    19741 gactttgcag aatcaggttc attagatttt gatgattttt tggaataata aatttagaca
    19801 ttatttaagc gagattaact gaatttaatc tcgcttaaat ttatttctat aatattgggg
    19861 gaatattatg aggaatacat ttattattgt ggtcaattat taaaatttta tacagtttat
    19921 tataaaaaat ttatactttt ctttatgttg tttgccaata ttagtctcaa atacgagtac
    19981 catactattc tgacccacca acacaagaaa gagtttttta aatgaaaaag tcagataaaa
    20041 ttaaatttta tctgactttt tctatgttta tcttattaga ttatgttaat atctgttgac
    20101 cgaataagcg catactcagt caactttcta tacctataat aatattgggg gaatattatg
    20161 aggaatacat ttattattat ggtcaattat taatgtttta tacaacttgc tcaaaaaaat
    20221 atatattaca tttcttcctc atcaaattct tcaatattag tagcctgtgg tataccttgt
    20281 atagacatat tactagaacc ataaggagta ggatatatat taggaggcat agtactaggc
    20341 ataactacag gcataccttg cattgtatta tgacatataa ctcctggtat catcataagt
    20401 acattaggag gcatattttg attacatgag aagttaggaa tacccatagt attattcata
    20461 ttatacccca tattactgtt catgttatta ggcacattgt taggcatacc catgttacta
    20521 ttcatattat taggcatatt gttaggcata cccatgttac tattcatatt attaggcata
    20581 ttgttaggca tacccatgtt actattcatg ttattaggca tattgttagg catacccata
    20641 ttactattca tattgttaga catattgtta ggcataccca tgttactatt catgttatta
    20701 ggcatattgt taggcatacc catattacta ttcatattgt tagacatatt attaggcata
    20761 cccgtattat tattcatgtt attaggcata ttatttgtag cagatgtatt gtaattcata
    20821 tttccggatg tattatttgt gttaggcaag ttgtttggag aattactact tttaggataa
    20881 tgatttacat tattaggata taggttattt atgttctggt tattaggtat ttttgtataa
    20941 ccattagaaa tttgagggtc atttctcaat tcatcttggg gaatttcttc attagtaata
    21001 taatctgcat agttcgctgg gtctaagtaa ggcatgtaac tagtatctaa atcctttttt
    21061 ttcttgttac tcatattaaa cttgcacctc ctagaaaact ataaaaaagt ttacaaaaac
    21121 ttattctaat aatatatata ttaaaaaagt ttataatatg tgcattctaa ttatattaaa
    21181 ttaataaaaa ttattgttgc aaatttagga atgatagcat ataatgaaca tatgaacagt
    21241 tattcatata ttaaaacaat ggaggattaa agatgagaga agagataaat gattgtaatt
    21301 gtaatatagt acatgaagaa atagtaacag aggctaaatc aactatgcca gatgaagaga
    21361 tgttatatga tttagcagaa ttatttaaag tatttgggga tactactaga gtaaagatat
    21421 tatatgcatt atttgcaaat gaaatgtgtg tatgtgatat agcgagttta ttaaatatga
    21481 ctcactctgc aatatctcat caacttagag tactaaagca ggctagatta gtaaagttta
    21541 gaagagaagg aaaaacagtg tattactcat tagatgatag tcatataagc cagatatttg
    21601 attgtggatt aaatcatatt agagaaactt ataaatgata aatgagagga gtgaatatat
    21661 atggaagcaa ataatttaat taaaaaagaa tttatactag gaggattaaa ctgtgcacat
    21721 tgtgcagagg agattaataa taaagtttct aaactacaag aagttaaatc ttccaactta
    21781 aattttataa ataaaaaact tatagtaaat ataaaggaaa gctttaatga ggatactact
    21841 atagaaaaga taatagatat aatagattca acagaaccag gtcttgatat acaaataagt
    21901 tctaaagaaa atgcatctag taaaacatca attaaaaaag agcttatatt aggaggatta
    21961 aactgtgcac attgtgcaga ggagattaat aacaaggttt ctaaattaaa agaagttgaa
    22021 tcttctaatt taaactttgt aaataaaaaa cttacagtaa atataagtaa taactttaaa
    22081 gaggatgatg ttataaataa aataaaagaa ataataaatt caacagagcc aggacttgat
    22141 atacaagtag ggtctactga caaagtaaaa gggagaacta ctgaaaaatc aggagctgta
    22201 aatgatacaa ataagaaaga attagttcca cttataattg gagcacttgt atatatattt
    22261 ggtatatatc aaacagctac aggttatgaa agtcagttta gcaatatagt atttatagtt
    22321 gcctatgtaa tagtaggtgg cgatgtgctt ttaagagcaa taagaaatat atcaaaagga
    22381 agagtatttg atgagaactt tttgatggct ctagcaacag taggagcatt aacaataggg
    22441 gagtcatcag aagcagtagg ggttatgctt ttctataaag taggagagta tttacaaggt
    22501 gttgcagttg gtaaatccag aaagtctata acttcactta tgcaaataag accagattat
    22561 gcaaatctaa aatttaattc tgaagttaag gtagtgtcac cagaagaagt aaatgtaggg
    22621 gatataatag tagtaaaacc tggtgaaaaa gtaccattag atggtgtagt agtggatgga
    22681 gtttctatgc tagatacttc tgcactcaca ggagagtcag tgctgagaga agttgaaaaa
    22741 ggagatgaaa tcttatcagg tgttataaat aaaaatgctt tattgagtat tgaagttaca
    22801 aagagttttg gagaatcaac agtttctaag attcttgacc ttgttgaaaa ttctagcatt
    22861 aaaaaatcaa agacagaaaa ctttatatct aagttttcaa gatactatac tccaatagta
    22921 gtaatagctg ctttattaat agcatttgtg ccaccacttg taatctcagg tgaggtattt
    22981 agtgattggt tatatagagg attgatattc ttagtcgttt cttgcccttg tgcattagtt
    23041 ttatcaatac cacttagttt ctttagtgga attgggtttg catctaaaaa tggtatcctt
    23101 attaaaggaa gtaactattt agaagcttta agaagtgtag atacagtagt atttgataaa
    23161 acaggaacac ttactaaagg tgtgttcaat gtaactaagt taaatcctga aggtatatca
    23221 gaagaagaat tattagaata tgcagctatt gcggaggtaa attcaaatca tccaatagca
    23281 aagtctatat taagttacta taataaaaaa attgatttag atacaataga cagttatgag
    23341 gaaatagcag cttatggaat tagagtaaag cataatggca attttatatt agcaggtaat
    23401 gagaaactta tgaaaaaaga aaatatatct tattcatcag ctaaagaagt aggcacagta
    23461 gtctatattg ctgttgataa agtatataga ggttatatag ttatatctga tgaagtaaaa
    23521 gaagatagta aaaatgccat aagaagctta aaagcaatag gggtaaaaga agttgtaatg
    23581 ttaactggag ataatgagaa agtagcaaaa aatattgctc aagaattaga attggataca
    23641 gtttactcaa atttacttcc taatgaaaag gtagatagat tagaagattt atatgaagga
    23701 agaactgaaa aagagaaaat agcatttgta ggtgatggaa taaatgatgc tccagtatta
    23761 gctcgtgctg atgttggtat agctatggga ggcttaggtt cagatgctgc tattgaagct
    23821 gcagatgtag tgcttatgac agatgagcct agcaagatat ctaaagcaat tgaaatagca
    23881 aacaagacta ataagatagt gtggcaaaac ataatatttg ctctaggagt aaaaattata
    23941 gttatgatac taggtgctgg aggagtagct actatgtggg aagctatatt tgctgatgta
    24001 ggtgtggcac ttatagcagt agtaaatgct atgagagcta tgagataagg gataaatgta
    24061 aatccatact atttaaaaaa cttataatta tatattgaag tatgtaataa tgaaaggtat
    24121 caataagttt cttttaactt tattggtacc tttttattgg agcgctttgc atatataaat
    24181 ttatattcat tatccattgt gtttatttaa tgtatttatt tagtgtagag cttttatatt
    24241 gtaattttaa aatttagaca tagaaggttt attaataagt ctatattaag tttaaattag
    24301 attcataaaa attataatac ttatgattgt atatatatta tattaatagt tataattttt
    24361 acaacaaaat gtgtgtgtta aaatactcta aaccaacagt atgactgtca ttcaataaat
    24421 tatgtaatat aagaaagtaa atatttttaa aaaataattt aaaaaagtat tgacgaaata
    24481 tttttaag
//
LOCUS       DAEPLF010000037        24291 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEPLF010000037 DAEPLF010000000
VERSION     DAEPLF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.037"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(327..1157)
                     /locus_tag="KR314_001328"
     CDS             complement(327..1157)
                     /locus_tag="KR314_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0327719.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPITYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKI
                     "
     gene            1230..2957
                     /locus_tag="KR314_001329"
     CDS             1230..2957
                     /locus_tag="KR314_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327720.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLIMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFVKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNREHFEKKRF
                     DRTAIDYSETAISINKVFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALI
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGRKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDVKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEIKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            2994..4856
                     /locus_tag="KR314_001330"
     CDS             2994..4856
                     /locus_tag="KR314_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327721.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKVKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRRAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFSSEYDEEVV"
     gene            5189..5878
                     /locus_tag="KR314_001331"
     CDS             5189..5878
                     /locus_tag="KR314_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBH0327722.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNNCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSIDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            5983..6669
                     /locus_tag="KR314_001332"
     CDS             5983..6669
                     /locus_tag="KR314_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBH0327723.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKYGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            6686..8593
                     /locus_tag="KR314_001333"
     CDS             6686..8593
                     /locus_tag="KR314_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBH0327724.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLTSVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETHKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDKKEVNAELTSSVVKT
                     NTDNETENKTTNDSESKNNTDKDKSGNDNNQKLEGPRGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            9046..9735
                     /locus_tag="KR314_001334"
     CDS             9046..9735
                     /locus_tag="KR314_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327725.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            10171..10710
                     /locus_tag="KR314_001335"
     CDS             10171..10710
                     /locus_tag="KR314_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0327726.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            10729..11772
                     /locus_tag="KR314_001336"
     CDS             10729..11772
                     /locus_tag="KR314_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327727.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            11774..12610
                     /locus_tag="KR314_001337"
     CDS             11774..12610
                     /locus_tag="KR314_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0327728.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            12715..13389
                     /locus_tag="KR314_001338"
     CDS             12715..13389
                     /locus_tag="KR314_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0327729.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            13389..14441
                     /locus_tag="KR314_001339"
     CDS             13389..14441
                     /locus_tag="KR314_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0327730.1"
                     /translation="MIKFKKTISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            14885..15733
                     /locus_tag="KR314_001340"
     CDS             14885..15733
                     /locus_tag="KR314_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0327731.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            15723..17387
                     /locus_tag="KR314_001341"
     CDS             15723..17387
                     /locus_tag="KR314_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0327732.1"
                     /translation="MMFKIFNKSLLKLVIVLSIVLTISNFSLFSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINENYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKVTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKYISNISKILIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            17436..18692
                     /locus_tag="KR314_001342"
     CDS             17436..18692
                     /locus_tag="KR314_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0327733.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYKVSKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIEGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTTVIISDILCVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            18715..20796
                     /locus_tag="KR314_001343"
     CDS             18715..20796
                     /locus_tag="KR314_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBH0327734.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASSFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGTNINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKPKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            20833..21918
                     /locus_tag="KR314_001344"
     CDS             20833..21918
                     /locus_tag="KR314_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBH0327735.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDGSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWVENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDSSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            21972..23096
                     /gene="wecB"
                     /locus_tag="KR314_001345"
     CDS             21972..23096
                     /gene="wecB"
                     /locus_tag="KR314_001345"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBH0327736.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKALVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDSYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            23174..24148
                     /locus_tag="KR314_001346"
     CDS             23174..24148
                     /locus_tag="KR314_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBH0327737.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSVTIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDIIRSYNLQLIDNKVETK"
BASE COUNT         9672 a         2440 c         4174 g         8005 t
ORIGIN      
        1 caaaagaaaa ggatgctttg atttgctata tatggaaaaa ttaaaaatat attcataatc
       61 aaatttataa aattaatatc tttccatcct aacattacat ttacttagat taattattat
      121 tataaaaata aaaatacaaa taatagattc atttacattc ttaaatgatt aaagctcttt
      181 accctttaaa catgcataac atatcgttat aattccagta aataagacta aaatgcccat
      241 tcctaaaatt gcagtttcat catctattac tccaaatgct tttaaagtaa cttatttcgc
      301 tttgtataat aataatttgt ctctttttat attttaactt ctgctgagat ttgagttaga
      361 tattcgttag aatctcgcac acttatatca tatagaagat attcttcata aacaaaatcc
      421 ccaatacgta aattattctg attaaaaaaa tttaataact tttcataagc cttgtaaata
      481 gtttcataac tacctttatg ataagcagtt acataaagtc cctttggttt gattgataca
      541 ggatatttct tgtactcttt attaactttt gtaaataaat atgaatatga ttcaaagtct
      601 ttattaatta tatcttcacc tttaatgatt gagccaatcg taccatagtc actcaattca
      661 tagttgtcca taaagttaat acattcatat aaaaaatttt cttcctcatc tcctaaaaac
      721 tgttctttat atgtaatagg tgttttgacc aagtactctt cctcatgttc ttttaaagta
      781 atagtattcg catctatatt acaggcattt ttagtgaatg aaatagtaga ttccattaga
      841 gcttgaatat tatttagttt ttcaatttca tttttaatat caatggtttt cttttctaaa
      901 agctcaatta gtaaattagg tgttcttttg tctatataag ctttaatttc ttttaaaggc
      961 atttttactt ccctaagtga agaaattata ccaaaaatgc taagttgatg gtatgagtag
     1021 tatctatatc cattttcttt ctttaattct ggtgagaaaa gacctatatc atcataatgg
     1081 aaaagtgttt ttttattgat tccacaaatc tttgcaaatt ctccagttgt aaaataattt
     1141 tttgaaactt gactcataat tataattcct ccttgactat cctgttacag tatagtttat
     1201 catagtattt tgtaaaggag tgtgattata tgaaaaaatt aattcatttt ttaaaaccat
     1261 atagagtttt aattgttgta gttttgatat ttacgttttt acaaacactt ggaactttgt
     1321 atataccaac acttacagca aatatagtaa acaatggtgt agtaaaggga gatatagatt
     1381 atattgtaaa aacaggttta ataatgatga tagtagctgg tataacagct ttatcagctg
     1441 tgttagtttg taaagtatca gcaaatcttt catctggatt ttgtagagat attagagaag
     1501 cagtatttgt aaaatcacaa gatttatcaa taaatgattt taataatata ggtacagcat
     1561 caatgattac taggagtact agtgatatta ctttaatagg tcagtctgtg tttatgttta
     1621 ttcaattggt attaccagca ccaattataa ctgtttctgg tctattctta gcatattcta
     1681 tagataaagc aatgacaatt attattgttg tagtaatgtt tttattcatg ctttcagcat
     1741 ttttagttgg taaaaagctt ataaagttgt ttaaaatgat gcaaataaag atggacaata
     1801 tgaatagggt attacgtgag gtagtaactg gagtaagagt tataagagct tttaataggg
     1861 agcattttga aaaaaagaga tttgatagaa ctgcaataga ttattctgaa acagcaattt
     1921 caataaataa agtatttgca gtacttatgc ctattgtaat gttaataatg aatttaggta
     1981 tagtaagtat tatttggttt ggtgggatga gagtcagtaa tggaaatatg gagataggtc
     2041 atataatggc acttattgaa tattgtatct taattttatt ctatttaatc atgggagtaa
     2101 tggtatttat gtacatacct agagcaggtg catgtgcaga tagagtaaat cagatattag
     2161 atatagaacc tgaaattgta gatggaaatg gtcgtaaaga tactgtctca gagaggggac
     2221 atttagtatt taaaaatgta acattttcat atgcacaatc agaagaacct gtgttaaata
     2281 atataacatt tgaagcaaaa tctggagaag taactgcaat tataggaagc actggctcag
     2341 gcaaatctac tattgcaaat ataataccac gtttttttga aattcaatct ggagaaatat
     2401 ctataaatgg tcaggatgtt aagaagattc ctcaaaaaga acttagagat aagattggat
     2461 ttgtgccaca aaaagctttt ttatttagtg gaacaataga agaaaatata aggtatggaa
     2521 aagaagatgc aagtattgaa gaaataaagc atgctgcaag tatagctcaa gctgatgaat
     2581 ttatttctga tatggaagac aagtatgact catttgtagc acaaggtgga aataatttgt
     2641 caggtggtca aaaacagaga atatccattg caagagcgtt agttagaaag cctgaagtat
     2701 atgtctttga tgatagtttt tctgcattag attttaaaac agataaaaga cttagaaaag
     2761 cgttgaaaaa tgaaatcaaa gattcatcag ctataattat agcacaacgt ataagtacaa
     2821 tcatggatgc taatcagatt atagttttaa atgatggaaa aattgtaggt attggaaaac
     2881 ataaagattt acttgaaaat tgtgaagttt ataagcaaat tgcggattca caacttagta
     2941 aagaagaact tgcataaaat gaagaattaa tctgatattt ggaggtgtat gatatgagtg
     3001 aaaaaagaat agatgaacaa gatatagaag tacttagttt tgaagatggc atgtcagcag
     3061 aaaaagttaa ggatgcaaaa aaaactacta agagattatt aaaatatatg gcaaaacaaa
     3121 agtttaaaat attaattatt tttatttcag tattgataag ttcagcgctt acagttttag
     3181 cacctatggt tatgggaaaa gctattgacc aactatttaa tggaataaaa actgcagtcc
     3241 aaacaggtac aaaattcagt gtgaatttta gtactatggg ggggatagta tcaatacttc
     3301 tagggttgta cttaattagt gcagtattta tatatattca acagtatatt atgtcaggag
     3361 ttgcacaaaa tctagttctt agcatgagaa aagatttaag tgataaactc aataaacttc
     3421 ctcttaagta ctatgattct cataaaaagg gggaaacact tagtatagta acaaatgatt
     3481 tagaaaaagt agcagatagt ttacaagaag gattaatgca acttattaca gcagtagtta
     3541 cagtagttgg ttctattgta atgatgataa gtataagtgt accacttaca atagtatcag
     3601 ctataacatt acttgtttca ttgggtatta ctgttgtaat tgcaagaaaa tcacaagaaa
     3661 gattctctga aaatcaaaag gcactaggag agttaaatag caatatagaa gagatattta
     3721 ctggtcaaat agtggttaaa gcatttagta aagaaaagga tactataaga aactttaaaa
     3781 tattaaatca aaatctttat aatgcaagta gaagagcaca gttttcgtca tatgcaatat
     3841 ctccaattat tagatttata aacctaattg gctatgttat tatagcagtt gtaggtggga
     3901 tatttgcatc aactggagca atgactcttg gtagtataca agcgtttatt caatatgtaa
     3961 atcaagcatc agagcctact acagaaatat cttatattgt aaatatgctt caagcagcaa
     4021 tagcatctgc agagagagtt tttacagtta tggatgaggt ggaagagata aaggatgaag
     4081 aatcttcaaa ggttattagc atgcctaaag gtaaagtaca atttgagcat gttaaatttg
     4141 gatatagtga tgattttatt cttatgaaag atattaatat caatctaaat gcaggagaca
     4201 agattgcaat tgttggacct acaggagctg gaaagacaac tcttgtaaat ttacttatga
     4261 gattttatga gatacaaggt ggaagaatta ctattgatgg tgttaatata aaagacttaa
     4321 agcgtggaga acttagaact atgtttggta tggttcttca agatacatgg ctgtttaatg
     4381 gaagtataaa agaaaatatt gcatatagta agtcagatgc cacaatggat gaaattgtaa
     4441 gagcagcaaa atcagcaaga gtagaccatt ttataaggac tttaccacaa gggtatgaca
     4501 ctatattgac tgaggatgct tctaatctat ctcaaggtca aaagcaactt ctaacaattg
     4561 ccagggcaat actttcagac ccatcagtat tgattcttga tgaagcaaca tctagtgtag
     4621 atactagaac agaggtagaa attcaaaagg caatgaataa cttgatgaag ggaagaacta
     4681 gttttgttat tgcacataga ttatcaacta ttcgagatgc tgatttaata ttagtaatga
     4741 aagaaggcac tattattgaa caaggaagcc ataaggaact tattgaaaag aaaggattct
     4801 atgaagaact ttataatagt caattttcaa gtgaatatga tgaagaagtt gtatgattat
     4861 caagttttag gcaactaaat gattgataaa tattaagtta aatatagtaa catgtaaggt
     4921 tatatggaat aagagtattt ctctatttca tataaccttt tttatttatc aaaataagat
     4981 aaaattttct ttctaaatta gattgaagaa agtatttatc aatataaaag taaaataatc
     5041 attattatta gtgaagtaaa tattgtttgc cacatatatg ccataaagat gcacttattt
     5101 tacggagatt taacaaagta gtgtaaagta tactctaaat ataaaataaa tcaaatagag
     5161 ttaaataaat tgagaggtga tataaatagt gaataaaaaa ttgaactata gatttgaaat
     5221 gaagcacaaa ataactgaag cagatgtgtt agctttaaaa tcaagactct atccaataat
     5281 gaagaaagat gaaaatgcat ctaaagatgg aaaatatctt attaggagtt tatattttga
     5341 tactcctgaa gataaagcac ttttagataa attaaatggc gttgcaataa gagaaaagtt
     5401 taggataaga ttttataata ataactgctc ttatataagg ttagagaaaa aaatcaaaca
     5461 ttacaacatg acatcaaagc tatcaactaa tttaaccaaa aaagaagtaa taaatatact
     5521 aaacaatgat attgaatttc taaaagagtc gacaaattgt ttattaagag aattttattt
     5581 gaaattaaaa tgtgaaagat tagaggcaaa aagcatagtt gattacaata gagaagcttt
     5641 tatttatcca gttggaaatg taagagttac aatcgatagt gatattaaaa cttctgtaaa
     5701 ttcgatagat ttatttaata aagatttgcc gactgtttcg gtaattgatg agaatatgac
     5761 tgtattggag gttaaatatg atgaatttat accagatttt ataaaggatt taattcaaat
     5821 taataagact acttctacag cagtttcaaa gtatgcttca agtagattat atatttaaga
     5881 ataaaggcta tgatttatat aaagatatac ataaataagg ttaaaaaaca aataggttaa
     5941 aaataaataa aataaaaaat aaataagtta aaaataggaa ggatgatttt aattatgaca
     6001 tttaatgata tttttaaatc aagttttata gaaaacgtat caggtttttc atttgtggac
     6061 agtgctttag cactaggttc agctttttta gtaggtttgt ttatatatat ggtctataaa
     6121 aagacttata tgggaattat gtattctcga ccattcaatg tgtcgcttgt agcacttaca
     6181 atgcttacaa cttttgtaat attggcggtt acttcaaatg tagttttatc cttaggtatg
     6241 gttggtgctt tatctatagt aagatttaga acagcaatta aagacccaat ggatttagta
     6301 tttctatttt ggtcattggg ttcaggtata gttttaggag caggtttgat tcccttagct
     6361 gtcatgggtt caataattat gggattgatt ctaatattct tttcaaataa aacaatatct
     6421 gaaactccat atatattgat ggtaaattgt aataatgaag attcagaaga tatagcaact
     6481 gataaaatta aaaaagtatt tactaaatat caaataaaat caaaaagtgt tacaccagaa
     6541 aaaggaatag aattagtgtt tgaagtaaga atgaaatatg gagagacaag tcttataaat
     6601 gacttgagtc aagtagatgg agttactaat gcagttcttg taagctataa tggtgactat
     6661 gtagcataga gaggagtaat cttttatgaa agataaaaaa tttacccttc ttatctctat
     6721 tatgattata tttttatgtg ctgtagttgg agtttatagt acatctagca acaaaagtgt
     6781 tgatttatat agtgatgtat atattgaaaa atattttaac agagacaagg ttatggaagt
     6841 taatatagag atagatgaaa gtgacttgaa ggatatgaat gaaaatgcta taaaagaaga
     6901 atttaaggtt gcaaaagtaa ctgtagatgg agatacatat ggaaacgtag gtataagaac
     6961 taaaggaaat tcaagtctta catctgtagc aaatagtgat agtgatagat acagctataa
     7021 gattaatttt gataagtata atactagtca aagtatggaa gggcttactc aattaaatct
     7081 taataactgt tactctgacc catcttatat gagagagttt ttaacatata gtatttgcga
     7141 ggaaatggga ttagcgactc cagaatttgc atatgctaaa gtctctataa atggcgaata
     7201 tcatggtttg tatttggcag tagaaggatt aaaagagtct tatcttgaaa ataattttgg
     7261 taatgtaact ggagacttat ataagtcaga tgaaggaagc tcgttgcaat ataaaggaga
     7321 tgacccagaa agttactcaa acttaatcgt tgaaagtgat aaaaagacag ctgattggtc
     7381 taaaattaca aaactattaa aatctttgga tacaggtgaa gatattgaaa aatatcttga
     7441 tgtagattct gtccttaaaa atatagcaat aaatacagct ttattaaacc ttgatagcta
     7501 tcaaggcagt tttgcccata actattattt atatgagcaa gatggagtat tttctatgtt
     7561 accatgggat tttaatatgt catttggtgg atttagtggt tttggtggag gtagtcaatc
     7621 tatagcaatt gatgaaccta cgacaggtaa tttagaagac agacctctca tatcctcgtt
     7681 attaaaaaat gagacacaca aaacaaaata ccataaatat ctggaagaga tagtaacaaa
     7741 atacctagat tcagactatt tagagaatat gacaacaaaa ttgcatgaca tgatagcatc
     7801 atatgtaaaa gaagacccaa cagcatttta tacttatgaa gaatttgaaa aaaatataac
     7861 atcttcaatt gaagattcta gtgataataa gggatttggt aataaagggt ttgacaacaa
     7921 taactctaat aacagtgatt ctaataataa ttctaatagt gaaaataagc gctctggaaa
     7981 tcaaagtgat aaaaaagaag ttaatgctga attaacatca agcgtagtca aaactaatac
     8041 agataatgaa actgaaaata aaactacaaa tgatagcgaa agtaagaata atacagataa
     8101 agataaaagt ggaaatgata ataatcaaaa gctagaaggt cctaggggta aaggaggtaa
     8161 gtcaatacca ggggttttgg aagttgcaga agatatgagt aaaactataa aatctcaatt
     8221 aagtggagaa acttcttcga caaagcaaaa ctctggtgat gaaagttcaa gtggaattaa
     8281 aggtagtgaa aagtttgatg aggatatgag tggtatgcca gaaccacctg agggaatgga
     8341 tggtaaaatg ccaccaggaa tgggtaatat ggataaggga gatatgaatg gtaaaaatgg
     8401 caatatgaat atggatagaa atcaagataa tccaagagaa gctggaggtt ttggcaatag
     8461 aggaggaggc tctgtgagta aaacaacaac atacttcaaa ttaattttag gtggagcttc
     8521 aatgataata atgtcgatta tgttagtagg tgtatcaagg gtaaagagaa gaagatttat
     8581 aaagtcaaaa taagattaaa attgaatata aataatttta atactgttaa gtctaaatat
     8641 ttacttagca gtattttttt agaaaataat gtgtttgatt aataatataa ggtaataaat
     8701 tgataaaaat taaatacagc atattaataa tacataataa agtaataaaa aaactgttta
     8761 agtataataa cttatattta gacagttttt ttcatttata aataatataa atgaaattga
     8821 aaacatttga aggatagaaa ttggttggat aaatgtataa attacttcac caaaaaataa
     8881 tgacaagagt tgtaactaag ttgtaataaa atggtagtta tttagcataa aataatgtat
     8941 catagaagta tattataaaa ttaataaaat aagatttttg taagaaaact gctggctttt
     9001 gatatttaga gtgagtagtt aaattatgtt taggaggggt agaatttgtt gcaaaaagta
     9061 ttatggaaat ttttaaagct agtagtcggt ttatttatat gttcagttgg tatagtattg
     9121 acgataaatt gtaaccttgg attatctccc tgggatgtat ttcatcaagg actttcaaat
     9181 catataggaa taactatagg aactgcttct ataattgtag gctctatagt agttattgct
     9241 gatgtcgttc ttggagaaaa tgtaggatgg ggaactgttt ttaacatgct actaattgga
     9301 ttctttatgg atttactttt atatagtaat ttaattccag aggctgatag tttatttgta
     9361 ggaataatta tgttgatatt aggattggtt cttttgtctg ttggaatggt attttatatg
     9421 ggaagtggtt taggaagtgg acctagagat ggtttgatgg ttgcaattca aaagaaaaca
     9481 ggaaaatcac ttaaattaat aagaggtact atagaagttg gagcattaat agtaggattt
     9541 ttattaggtg gaaaagttgg tataggaact ataataagtg catttggatt ggggtatttc
     9601 acacaaatgg tatttggctt atttaaatta gattgtagta agattaaaca tagatttata
     9661 gtagatgata taaaatttat caaagtgtat atgagtggtg agaaaaaagc tgctgttcaa
     9721 aatatagtta agtaatttaa gaataaattc aacgaaatgt taagtatttt aaattaacat
     9781 tttatttaga tggataaatt gaatattaaa agaatattaa aatatgagga ttgctagaac
     9841 actataaaat atagatttct agcaattttt tatttgttca atgttttttt agtactagaa
     9901 gtcttattct ctatttaatg aatgaattaa tttataatta gagatactaa actttataga
     9961 tattaatata aacaccttta aactgtgtat tttccaaagc ctttaaaatg cttgtattta
    10021 ttctaaataa aaaaataaaa aaatgtcaaa aaaactcttg tattatatcc aaattttata
    10081 tataattacc tatgttgttt agaaatttta aactcaatgt ttagtaaaca aaatgtttag
    10141 taaaataaaa cagaaactag gaggtgcaac atggatatag gtgaaaagat aaaaagatta
    10201 agaacagaaa aacaactgac ccaggaagag ctagctaata gatgtgaatt gtcaaaggga
    10261 tttatttctc aattagaaaa taacttaaca tcaccatcca tagctaccct tatagatata
    10321 cttgaaatac taggtacaaa tttaagggaa ttttttaatg aaatagatga tgaaagaatc
    10381 agttttacaa aagaagatat gtttgaaact gaagatgaag atttaaaata taaattaaag
    10441 tggttaattc caaattcaca aaaaaatgaa atggagccaa taataataac attgtatcca
    10501 ggaggacagt ataaagaaga aaagccacat gaaggtgaag aatttggata tgtattagca
    10561 ggttcaatat atgtccatat aggcgaaaag aaaaataaag taaaaaaagg agagagtttt
    10621 tactttagac caaaagcaaa tcactatata tcaaacgaag gcaaaactac agcaaaagtg
    10681 atttgggtaa gtacgccacc atcattttag aatagaggtg tttaataatt ggtagaaaat
    10741 ataatagaat taagggatat atctaaacat tatgaggaac ttactgtttt agataatttc
    10801 aatttagata taaagaaaaa tgagttttta acattacttg gaccaagtgg atgtggaaaa
    10861 actacaacat taaaaataat agcaggattt gagtatgctg atgatggtaa agttttattt
    10921 gaaggtaagg agataaataa cttgccacca tatgaaagac aagtaaacac agtatttcaa
    10981 aaatatgctt tatttccaca catggatgtt tatgaaaatg tagcttttgg attaaaaatt
    11041 aaaaagactc caaaagatgt aatagatgct aaagtaaaag aggttttgaa acttgttgca
    11101 ctagaaggtt ttgaaagacg acaagttgaa tcattgagtg gaggacaaca acagagaatt
    11161 gcaatagcaa gagctctagt caatgaacca aaggtattat tactagatga accacttgga
    11221 gcacttgatt taaagttaag acaagagatg caaatagaat taaagagaat ccaaaaaaaa
    11281 ttaggaataa catttgtttt tgtaactcat gaccaagaag aggctcttac tatgtcagat
    11341 actattgttg taatgtataa gggcaaaatt caacaaatgg gaacaccaca agatatatac
    11401 aatgaaccta aaaattcatt tgtagctaaa tttataggtg aaagtaacat atttgatgga
    11461 attatgatag aagattataa agttaatttc tgtaatagag attttgagtg tgttgataaa
    11521 ggattcgaaa aaaatgaaaa tatagaagta gtaataagac cagaagatat aaaaatggta
    11581 aagccagaag aaggaatgtt aaaggggaaa gttacatcta cagtgtttaa aggagtgcat
    11641 tacgagatag aattgaatga aaatggaaga ttgtggatac ttcataatac caaaaatgct
    11701 gaggttggga ctgaactagg catggatata tatccagaag atatccatat aatgagaaaa
    11761 gagaataact aagatgaaaa gaaaatcttt tttagcatat ccatatgtag tttggagtgc
    11821 gatatttgta ataattcctt taatattagt ggtatttttt agttttacaa aagaatcagg
    11881 tggaggttat gcctttacac ttgagaatta taaagaagta attgacccta tatatatgaa
    11941 agtttttggg cgttctattc ttttagcagg aggagctaca ttaatatgcc ttatagttgg
    12001 ttatccagtg gcatatataa tttcaaaggc tcgtgtaagt agaagaggtt cattaatatt
    12061 attgtttatt cttcctatgt ggatgaactt tcttttaaga acatatgcat gggttgctat
    12121 attaggaaaa aatggacttt taaatacatt tttaggttgg tttggaatac aaccattagc
    12181 aattttgtat actaattttg ctatactttt aggtatggtg tataactttt taccatttat
    12241 ggttcttcca atatatacag cactatcaaa gatggataat gatttgataa acgctgctca
    12301 tgacttgggt gcaaataata tgacagtttt tagaaaaatt atatttcctt tgagtttgcc
    12361 aggtgtaatg tctggaataa ctatggtatt tatgccagca gtcactactt ttgcaatatc
    12421 aagactgtta ggtggaggta aaataatgct ggttggagat ttgattgaac aacagtttac
    12481 agttgttgga gattggaact ttggttcagc tatatcgata tttatgatga tagtaatatt
    12541 aatatctatg tctataatgt caaaatttgg agatgaatct gacaaagaag gcggtgggtt
    12601 actattttaa aattaaagaa aataacttct aatatatatt tagcattagt attcttattt
    12661 ttgtatgcac ccatatttgc tttggtacta ttttctttca atgattcaaa atcaatggca
    12721 agatggaatg gatttacatg gaagtggtat ggtcaattac ttcaaaatga gagtataatg
    12781 agtgctttat attatactat tgtaatagct atattagcgt ctgtaatatc tacaatagta
    12841 ggaactataa gtgctatagg aatacataag atgagaggta agagtaagaa attaatacta
    12901 aatgtaaact atcttcctat tttaaataca gagatagtta cagcagtagc tcttatgagc
    12961 ctgtttgtat ttgtaaaaat ggaatttgga tttacaacaa tgctattggc acatataatg
    13021 ttttgtttgc catacgttat actttcagtt ttgccaaaaa tgaagcaatt acctgataat
    13081 atagaagatg cagctatgga cttgggagca acacctatat acgctcttag aaaagtaatt
    13141 ttaccacaaa taaaaccagg tatagtttct ggatttttaa ttgcatttac tatgtcaata
    13201 gatgacttta taataagttt ctttaatgct ggaaatggtg taagcaatct ttctatagaa
    13261 atctatggaa tggctagaag aggtataaaa cctgaaataa atgcattatc aacaataatg
    13321 tttgcagtgg ttctaggttt acttttacta gctaataaaa aagaatctat agtaaggggg
    13381 attaaataat gattaagttt aaaaaaacaa tatcccttat agctgtaggt atgatgacag
    13441 ttactttatt tacaggatgt aataaagcaa ctgattctac aaaagtttta aatgtatata
    13501 atgttggaga ttatatagat gagtctctta tagataagtt tgaaaaagaa acaggaatag
    13561 atgttcaata ttcaacttat gatactaatg aaatgatgta tcaaaaagta aagagtggta
    13621 gtacacacta tgatttagta tttccttcag actatatggt agagaaaatg aaaaatgaag
    13681 gtcttttgga aaaattagat tttaagaata tacctaatat gaaatattta gataagagtt
    13741 ttttaaatcc tgtatacgac aaaactaatg aatatagtgt tgcatatatg tggggaactc
    13801 ttggaatatt gtacaataag aaagaagtta aagaccctat ggatagctgg aatatacttt
    13861 ggaatccaaa gtataaggga aatataatga tgtttgactc tgttagagat actataggga
    13921 taactcttaa aaaattagga tatagcatga atagtgtaaa tccaaaagaa ataaatgaag
    13981 ctaaaaatct tttaatgaaa caaaaggatt tagtattggc ttatgtaaat gatgaaggta
    14041 aggatagatt gcttggtgga gaagtggcaa tgggtatgct gtattctgga gatgcagtta
    14101 cgctaatgga gcaaaatcct gaccttgatt atgctatacc taaagaggga actaataaat
    14161 gggtagatgc tatgtgtgta cctacaacag cacaaaataa gaaagaagct gagctgttta
    14221 taaacttttt attagaccca gagaatgcaa aagtaaatgc tgagtacatt gggtattcaa
    14281 cacctaatac aggagcacta aaattattag acccagaaat aactcagaat cctgttgcat
    14341 atccatctaa agaagtactt gataaatgtg aaacttttga agatattggt gaaaatataa
    14401 aattatatga caaggcttgg atagagttaa aatcaaaata gggtcttgtt ggtttaagta
    14461 aatttactta ataaatactt aataaaactg ttctttttta gaaaatttat tttctaatta
    14521 ttgaacagtt ttattttttg tttaggtcat gtttttattt tgagtttatt tttatgagaa
    14581 tttaaagtac agtaagtttc attgattact atttttataa taataactat aaagaggttg
    14641 tctcgaaatt gagataagcc tctttttcat tgttgaaatg atgaaaacat gtttgatttc
    14701 ataaaaaaag agtgattcat gagctaaaga atttgctttg agacagtatt ttttattaag
    14761 ttatattcat atgcaattta aatagtttat taataaatga aaacttattt gtataatgat
    14821 tttaataata attagaaaaa atgggcaact taatagaata attggaggtg taaatttata
    14881 aaaaatgaat aggataaata aaaaaataga tttattaata ttggctttgc tcgtttttat
    14941 atttttaatt gttggaatag taatactatc gattaggaca gaaaataaat tgaatgtgtt
    15001 cataatgtta gcaataattt tttttataat aatgttaact tatttatcaa attcagtagt
    15061 ggggttgata acaagttcaa taattatatt tatgtataca tcctatattt tatataacaa
    15121 tattactcac aatatggatg ttgaatttat atcatatatg tggataatag caacaccagt
    15181 atcttcaatt ataatgggta atctgaacaa aagcataaat gaattgcaaa atacaaataa
    15241 aaaactatct gaacagtata aggaattagt gactattgat agtgaaactg gacttagaaa
    15301 tttaaagatt ttttataatg atgtaaatat ggagataagt aaatctatta gacataatac
    15361 tgatttttct cttatgatag taaaattacc atactacgga aacctacaga ctatatttgg
    15421 tgaaaacaaa actaataaaa tagtaaaata tattggttca aatattatag aatgtacaag
    15481 aaatgaagat ataatttata gtttacaaaa agatatgata gggatactaa tgccaaatac
    15541 atctttggaa ggttcgaaag ttgttaaaga cagaataaag aaaaggataa aagaattaaa
    15601 tttagattta aacaataggg gaaaatatgt aaatatagat gtcaaaattg catttttaca
    15661 atataagagt agttttggag atagtataaa ttttaagaat atagttgaag aggaacttca
    15721 atatgatgtt taaaatattt aataaaagtt tattaaaatt agtaattgta ttatcaatag
    15781 tactgacaat atcaaatttt agcttatttt catatgcatc agaaattact tccagcacag
    15841 ataaggtatt aatcatatat gattctaaga aagaaacagc ttataataga gatattttaa
    15901 atattatgag aactctattg ggaagattta gttcagatat agagctatta aaattgagta
    15961 actatgatgg tgagataaat gaaaattatt acagtcatat atttatttta ggaatcaatg
    16021 aaaactcata taataatgat aaaaacacta aaaacttgat tagttcctta aattcatata
    16081 aagtaactat atgttggtta ggatatggta tagaaaatct tttggaacat aaaaaataca
    16141 atttagacta tgtaggaaaa acaaacaata tagttagtgt aaattataga ggtaaatcat
    16201 ataacttgga tgaacattat gtttttaaca ttgtagaatc aaaagataca agtaataaag
    16261 taataggaag tattaatgat accttaaata agtatccata tataataaat gataaaaatc
    16321 ttttttatgt atcaaaatta gatttagatg gagttttatt ttatatattt tgcgattcac
    16381 ttaatgatat ttttaatatt aagacgtttg ataaaggacg tatatttgta agaatagaag
    16441 atgtacatgc ttttagagaa ccaaaaaatc ttgtagagat agcggattat ttaagtagta
    16501 agaatatacc atttacgatt gcattaatac cagcatatgt aaatcctaaa aatcataaag
    16561 taataacttt atcagaaagc cctgaaattg tcaaagcaat aaaatatatg caagataaag
    16621 gtggaactgt gatattgcat ggatatactc atcaatacaa gaaagaagaa gtttctggag
    16681 aagggtatga attttgggat ggtaaaaaag atgaaccctt aaaagaaaat atgaagatat
    16741 ttgtcaaaga tagagttttg aatggtctta gagtatgtat agagaatgga atttatcctc
    16801 ttgcatttga agcgccacat tatgcaatgg aatcagaggg atataaagaa ttaaaaaaat
    16861 atttttctac ttatatgggg caacatcaaa ataatgataa aaaatttagc acaaatacat
    16921 atccttatat tattagggat acagaggagt ttaatatatt tatacccgaa aatttaggat
    16981 atatagaccc agaagacaag tttacttttc aaaatataaa agaaaattta gataaattat
    17041 ctattgtaag aggttttagt ggagggttct tctttcattc atatttaaac atagagtatc
    17101 taaaaaacac aatagaatat ttagaaaaac agaatataga atttatgaat ttaagagatt
    17161 ttaataattg ggtaaaagtt gatgaaatac aaataagaaa taatggagat gaaattatag
    17221 tcaactatga taaggactta gatgaaataa ctaaaagtga tactagattt aaatacatat
    17281 ccaatattag taaaatttta atattcatag tatctataag tgttttaata tttgtgatta
    17341 tatttatata ttttaagaga atagataaaa agaaattttt aaaataaatg attttatatt
    17401 agaatcaata ttttaaaata ttttaggaga aatttatgag agatttaaat gtatcagagt
    17461 atttgttcgt atttagtctg tttagtatat ggtctttatt gcttataaat attattttag
    17521 ctatgggtgg atatatattt tattttaaaa actttgataa agaaataaaa gaaattgatg
    17581 aatatcctat gatatcaata cttgttcctg ctcataatga agctaaagtg ataggtagaa
    17641 ctgtggagtc tttgttatta ctcaattatc ctaagagtaa gatggaactt attgttataa
    17701 atgacaattc ttctgataat agtaaagaaa ttttagaaaa tataaaggat aggtacaaca
    17761 attataattt cactataata aatacagata gtttaacagg tggaaaagga aagtctaatg
    17821 cattaaatat tggatataag gtttcaaaag gagattttat agctgtttat gatgcagata
    17881 acacaccaga taaaaatgca cttagatatc tagtacaaac tatagtaatg aatgatgaat
    17941 taggtgctgt tattgggaaa tttaggactc gaaataaaaa taaaaaccta ttaaccaaat
    18001 ttataaacat agaaacatta agctttcaat ggatgtcaca agctggtaga tggcaacttt
    18061 ttaatctatg tacaatacca ggaactaact ttatattgag gagaagtatc atagaagaaa
    18121 ttgaaggatg ggatagtaaa gcaattgcag aagatacaga aataagtttt aggatatata
    18181 aattgggata caagataaag cttgtaccac aatctataac atgggaacaa gagccagaaa
    18241 ctgttaaggt gtggataaag caacgtacta gatgggcaaa agggaatata tatgtattaa
    18301 tgaaatatat aaaaaatata tttaaacagg gaagaaataa gattgtgttt gatatagctt
    18361 actttttttc tgtatatttt ctatttttaa caactgtaat tatatccgat attttatgtg
    18421 ttttaagtat atctaaacta gtagaaattt ctattccaat taactttttt ctaatatgga
    18481 tactatctta tcttttattt attatagaag tgagtataag ccttactata gaaaaaggtg
    18541 aagcaacaat tgaaaatata ttcatagttg caataatgta ttttacatac tctcaactat
    18601 ggctgtttgt agctataaaa gggatgatag agtatctaaa agatataatt tttaaaagag
    18661 aggttaaatg gtataaaaca gaaagatttt gattcatgta caggaggaat taacatgaaa
    18721 aaatttatta tatcaattat atctttagta ttgttttttt caaatatatc acttatatac
    18781 aaagtaaatg ctgatgagac taaagttaaa aattataagt ttgaaagaga tataactatt
    18841 gatggtgtta taggaagtaa tagtactttt tttgaagtaa ataaaaattg ggatattgaa
    18901 gaagttttat tgcatcttaa ttttagtaag agccaaatat taaatggaga tgtttcaagt
    18961 ttaactgtat tgataaataa tgtaccaata aaatctatta aattaaatgc taagacaaat
    19021 tataaaaata cattagaagt attagttcca aaagattata taattcaagg atataatgaa
    19081 ataaaaataa aaacatacaa aacaatatct gataaaattt gccaagatga ttccaatact
    19141 gggaactgga tggttattca caaagaatca tatatatcaa ttagatataa gcagaaaaag
    19201 gtggaaaact caataaatga atatccgtat ccatatgcag aaattgaaaa taatcataaa
    19261 ttagatacaa ctatagtagt accagataat atgactagag gagagactac agcagtgttt
    19321 aatttggcat catcatttgg aaaaataaca aaaaatgatg atttaaagtt agatgttaaa
    19381 ttatattcag aaatgaaaaa ttggtcagat gataatatta tatatattgg taaacctgaa
    19441 aatacagcag aagaaatttt ggatatttta agtataaagg aacaaaccct attatcttca
    19501 aactgtataa tcaagcaagt agattcacca tacaataaga ataagaagat gatggtggta
    19561 attggtagta atgaagatga tttaataaag gcatcaaatt tattaataga aaatagactt
    19621 tctaaccaag ttttatcatc atcagtgctt gtaaacaaag agactaatat aaaaattaat
    19681 agggaacaaa aattaaattt aggtcattta acattaaaag acttaggata ttctgacttt
    19741 ttgttggaag gagcatttaa tcaacaagct ttgtttgatg tcaaaatacc aacgggtaag
    19801 gtattagatg atggttcaaa gattatctta aatttaagat attctgacaa tttagatttt
    19861 gaaaaatctt tagttacagt gtctataaat gatgttattg tgggaagtaa aaaattggat
    19921 aggtcacatt ctaataatga taagttagaa ttaaaaatac ctaaagatat agataataaa
    19981 aactattatc aagttaaatt gacatttaat ttgagtataa aaaattcgaa ttgtgttact
    20041 agagagagta ataatccttg ggcatatgta tcaaataatt cttatttggc gttatctacg
    20101 aaagaaaatg aaaccttatc ttttgaaaat tatccgtatc catttgttag agatgatgag
    20161 tttaatgatt taactgtgat aatgccagat tattcagggt cacaagctat gacctggatg
    20221 tttagattag gtgttacttt aggtacgaat ataaattcac ataatggaaa tataaatgta
    20281 attagaggaa aagaatttag tgacaagtat aaggatacta atattgtagt ttttggagtg
    20341 ccacataata attctgttat aaagatgtta aataataatt taaatataaa gttcgataaa
    20401 aattactcca attttatatc aaatgataag ataagcttta ttgatgatta tggaaaaaat
    20461 atttctacca ttcaattaat aaaatcacca tataataatc aaaaaaatat catggtaatt
    20521 agttcaatga atgaaaaaaa cttatattta ggaatggatt atttgttgaa taaaagtaaa
    20581 gttaatgatt taaaaggaga tacattgata attgatgaat acggagaggt agaagattta
    20641 gcttataatt taaaacctaa gaaagaagta aaagattcaa gctggaatat gagtataaat
    20701 aagacaacta aagtattttt aatgatttct tttattacta taattgtagt catgatatta
    20761 tcaatgctat atataaaaaa atataaaaga agataatatg ataaatttat tattttaaaa
    20821 gaggtatata atttgaggaa atatatgaat aaatttaaat taaaaagaag cattttaatc
    20881 tcattaataa tcttaatttt tatatttata gtgtactttg taagtattga gttaaaagat
    20941 gggagtctag taaataatca atataaaggt aagattaaat cggccaattt atctgtagat
    21001 tatgaaataa atcaagttat gaaggatata gataaattgg gattgaatac agtaaatgta
    21061 cctataatta taaatgtaga gtccattaat tctgatatta tgtcaataga taataatagt
    21121 aaagaaaagg caattaagtt aataaaaaaa ttaaataaaa aaggtatttc aacaatatta
    21181 gaggcatatc catgggttga aaatggaaaa ttgtatgaga ctgactggaa ccctataaat
    21241 aaaaaaagat ttttttatac ttggcaaaat gccatattaa atgaacttat aattgatgta
    21301 gcaaatccac tggatgtaaa tgttttaaat attggttcta attttgtaca tttggaagaa
    21361 tatcaacaaa attggggtga aataatagat tttgtacaat ccaaatttga tggtcttgta
    21421 acgtacagaa ctaattggtg gtatacaaaa aaagatgatt caagtagtaa gttgttttat
    21481 gaacaaaaat tgaacaataa tttttttgat aagttagatt ttatatctat agcagcatat
    21541 tttgagttaa gtaataaacc tgttaataca gtggatgaat taatttctgc cttacactca
    21601 tctactgtaa acaatagagg acaaaatata aaacaagaaa tatataacct ttataaagct
    21661 catagaaaac ctatattttt tggagaatta ggtttttcaa atagagaaag tgcaagttct
    21721 cagccgtgga atcatactcc ttcaaaagct gtaaatggcg aagaacaagc aaggtgtttt
    21781 gaggcatata agaaagtttt tgaaaatgaa gactggataa atggtttttc tgtgttttgt
    21841 gtaggtaaaa tagatgacga aaaaaatttt tacccatcaa aagagagtat aaaagttatt
    21901 aagtcttggt atgaataatt ttaaaagtgt agaatttata tagattaaat agatgattag
    21961 gagagatata aatgaatgac atcaaggtta tgactgtatt tggaactaga cctgaagcta
    22021 tcaaagtagc acctttaata aaggaacttg aaaaaagaga gaatataaaa agtattgttt
    22081 gcgttacagc tcaacataga gaaatgcttg accaagttat agaaaccttc aatataaatg
    22141 tagactatga tttggatata atggaaaaag gacaaagcct gaatgatata acatgtaaga
    22201 ttttaaataa acttcccttg attttaaaca aggaaaatcc aaatataata ttggtacatg
    22261 gagatacaac cacaactcta gctacaagtt tgactgcatt ttataataaa gctcttgttg
    22321 gtcatataga agctgggctt agaacctatg ataaatattc tccttttcca gaggagttaa
    22381 atagacaact tacaggtatt attgctgata tgcactttgc tcctactaat ttggctagaa
    22441 aaaacttgat ttctgaagga aaacctaata ataatatatt tgtaactggt aatactgcta
    22501 ttgatgcttt aaagatgaca ataaaagaaa actataatca tcctataatt gatgagattg
    22561 gtaacgatag gatgattttg ttaacctctc acagaagaga aaatttgggt aaacctatga
    22621 aaaatatatt cagagctata aagcgaatag tagatgattt tgaagatgta cagatagtat
    22681 atcccataca tttaaatcct aaaatacgaa ctatagctga tgagatattt ggaaaatttc
    22741 ctgaaaaaat tcatattata gagcctttag atgtagctga ttttcataac tttttaaata
    22801 aatcttatat gataatgact gatagtggag gaatacagga agaagctcca tcattaggta
    22861 agccagtcct tgtgttgcgt gataaaacag aaagaacaga aggaattgaa gcaaagacac
    22921 ttaaattagt aggtactaat gaagatagaa tatataattc agtgagcgat ttattaataa
    22981 ataaagatag ctatgttcag atgagtaaag catctaatcc atatggagat ggaaatgcaa
    23041 gcaaatatat agtggatata ataattaaaa aatttaattg caaatattta aattagataa
    23101 ggtctaaaac attaactaat tgatataaga tttatgataa tattaaggaa tacaattttt
    23161 aagggggagt aatatggcta gaaaattaat aaaaaattta ggtaaaagta aaagtgtaaa
    23221 gagagtgaaa cttttattta agaagatttt tattactgta ttcatagtag caagtatagt
    23281 tgctattttt aatataacga aatattttga ggaattatac aaagtaaggg atttaaaaag
    23341 tactaaaatt gaatactata tggatgtggc tgatgaagca ggagatggaa aggtccaatt
    23401 aagttggaag gccttacttg ctatagacat ggtaatacat gatgaagatt taagtaatat
    23461 aaaaaagaaa gacacattgg atatagggga aaagtttata gtagaagata aaaatgataa
    23521 aggcgaaaag gtgtataagg taaaaaagtt taataaggta ttaagcgaat tgaaatttga
    23581 ctcttctcaa aaaagtagag caagaaaata catgaaagat ttggaataca cataccttgg
    23641 aaataaacaa ttagatagta gtgatgaaaa aattaaattt ataaagaagt tagaagactc
    23701 agctataaga gaatatattg attatggaat attgccctct gtaacaattg gacaagctat
    23761 attagaatct ggctggggaa attctaaact tacaaaacag agtaataatt tatttggtat
    23821 aaaagcagat aaagcatgga aaggaaaaag tgtagaaatt tcaacttcag agcattataa
    23881 tgaaaaaatt gtagctagtt ttaggtcgta taattcatta caagattctg tcaaagacca
    23941 cagtttattt ttaattaata ataaaagata tagaaaacat gggttgtttg aggcaaaaga
    24001 ttatattagt caagctcaag cattagaaaa tgcaggatat agtacagcag aagataaaaa
    24061 aggaaatcgt atatatgcag agctattaat agatattatt aggagttata atttgcaatt
    24121 gatagacaat aaagttgaga caaaataatt gttaacttta tatgaggaga taacaagata
    24181 agattctttt ctacaaaaag aatagaaaaa ttgtaaaaaa aactaaaaaa atgtattgac
    24241 ttgttattta aaaggtgata ttattattac tgtactcgaa agagaacaaa a
//
LOCUS       DAEPLF010000038        23363 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEPLF010000038 DAEPLF010000000
VERSION     DAEPLF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.038"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            364..1596
                     /locus_tag="KR314_001347"
     CDS             364..1596
                     /locus_tag="KR314_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBH0327738.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            1610..2416
                     /locus_tag="KR314_001348"
     CDS             1610..2416
                     /locus_tag="KR314_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBH0327739.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKHGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(2528..3397)
                     /locus_tag="KR314_001349"
     CDS             complement(2528..3397)
                     /locus_tag="KR314_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0327740.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            3768..5195
                     /locus_tag="KR314_001350"
     CDS             3768..5195
                     /locus_tag="KR314_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBH0327741.1"
                     /translation="MKLTKSISSMLCLALVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFLNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEIFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(5271..5540)
                     /locus_tag="KR314_001351"
     CDS             complement(5271..5540)
                     /locus_tag="KR314_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327742.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGISINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(5543..6985)
                     /gene="nhaC"
                     /locus_tag="KR314_001352"
     CDS             complement(5543..6985)
                     /gene="nhaC"
                     /locus_tag="KR314_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBH0327743.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAATKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDILNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(7010..8176)
                     /locus_tag="KR314_001353"
     CDS             complement(7010..8176)
                     /locus_tag="KR314_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0327744.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESVLVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIK"
     gene            complement(8401..10521)
                     /locus_tag="KR314_001354"
     CDS             complement(8401..10521)
                     /locus_tag="KR314_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0327745.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVVDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHIARKILSINANEMIDSFVGDCIKDINFQNILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERAADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLGK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            10754..11155
                     /locus_tag="KR314_001355"
     CDS             10754..11155
                     /locus_tag="KR314_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327746.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            11198..11491
                     /locus_tag="KR314_001356"
     CDS             11198..11491
                     /locus_tag="KR314_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBH0327747.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            11506..11946
                     /locus_tag="KR314_001357"
     CDS             11506..11946
                     /locus_tag="KR314_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBH0327748.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(12038..12511)
                     /locus_tag="KR314_001358"
     CDS             complement(12038..12511)
                     /locus_tag="KR314_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH0327749.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(12511..13299)
                     /locus_tag="KR314_001359"
     CDS             complement(12511..13299)
                     /locus_tag="KR314_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH0327750.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            13627..14871
                     /locus_tag="KR314_001360"
     CDS             13627..14871
                     /locus_tag="KR314_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBH0327751.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGIHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLINKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            15194..16291
                     /locus_tag="KR314_001361"
     CDS             15194..16291
                     /locus_tag="KR314_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBH0327752.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEITIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            16282..16980
                     /locus_tag="KR314_001362"
     CDS             16282..16980
                     /locus_tag="KR314_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0327753.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            16983..18047
                     /locus_tag="KR314_001363"
     CDS             16983..18047
                     /locus_tag="KR314_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBH0327754.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            18230..18934
                     /locus_tag="KR314_001364"
     CDS             18230..18934
                     /locus_tag="KR314_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0327755.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            18927..20249
                     /locus_tag="KR314_001365"
     CDS             18927..20249
                     /locus_tag="KR314_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0327756.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            20436..21809
                     /locus_tag="KR314_001366"
     CDS             20436..21809
                     /locus_tag="KR314_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327757.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIVMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(22182..23120)
                     /gene="pdaA"
                     /locus_tag="KR314_001367"
     CDS             complement(22182..23120)
                     /gene="pdaA"
                     /locus_tag="KR314_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBH0327758.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDGGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         8599 a         2915 c         3695 g         8154 t
ORIGIN      
        1 tatatatttt cataaattat ccttgtattc tcagccaata taattaaaat actatgttta
       61 aaaattttac ataaacttga ttgttttaga aattacttct gtaatgagta cacgatttta
      121 attaaagaat atataacaaa tttgaaataa gtctaatata tgacttaata gattaaatta
      181 aaataataaa tttggactat ttatctattt ataacttaaa ttatatcaaa ttgataacaa
      241 actagatatt atactttttt gtagaataga attacaatta tataaagaaa aattctatta
      301 atagtgaaaa aaaagtagaa aaaagtagaa attattcttt tataaagaaa gggagggata
      361 atattgcaag agagagaaga attagtttta aatccagttc caaaagagga aagagttgga
      421 tggttagctc cattgtttaa tatgcttggt tctaacatag ctatatctga actaatggtt
      481 ggaggaactt taattttagg aatgactctt tctaatatga ttattacatc aattataggg
      541 aacttaatat tagttgcaat cattatgata caaggttata taggttacaa agaagggtta
      601 aatacgtatg tattagctaa gggggcattt ggagagatag gaggaaagta tcttatatct
      661 ctattacttg gtataactag ttttggatgg tttggtgtac aagcaggggt tgcaggacta
      721 tcagtacaaa agatattccc aagtgtaaac ctaacattag ttacagttat acttggatta
      781 tttatggttg tttttgcact gtatggcttt aaagctatgg ctaaatttaa ttacttagtt
      841 ataccaccac ttatgatatt aatggtgtgg ggagttttta aagctttttc tacttatgga
      901 gttgaagcta tttataatta tactcctcaa acaactatga gtatggttga aggtcttaat
      961 attgtagtag gtcttgttat agttggtgca attatatcac ctgaccagtt aagatatacg
     1021 agacgagtaa aagatatttg gattatcagt tttttaggtc tagggattat ttcattattc
     1081 caacaagttg cagctggtgt aatgtctatg ggagcaccta cttgggatat aacagaagta
     1141 ttggctaatt taggttttgg atgggtagcg tttgttattt taatacttgc atcttggtca
     1201 acaaacgtat caaatgctta ttctggtgga ttagcattaa aaactatttt tccaaatgta
     1261 aaaagaaatg ttttgacttt agttgcaggt ctaataggta caataatagc agctactggt
     1321 ataatattta aatttcagtc atttttatct ttcttgggaa tcgcagttcc tgcaatagca
     1381 ggaattatgt ggtgtgagta ttattttata caaggaagaa cttataagca tagagaagga
     1441 ataaattgga ttgcagtaat aagctggtta ataggttttg cagcgtcgta ttatagttca
     1501 aaaataaatt tcttaatacc tccgataaat ggaattgtag tgagtatggt tatttattat
     1561 atattaatga aatgttttgg aatcaaagat aaataaaggg ggcaattaca tgggtcgtaa
     1621 ggtgaaaata ggtataattc aacaacacag tgtacttgga gatgttaaaa aaaatataga
     1681 aaaagcagta gagatgatag atgacttagg aaaacatggg gcagatataa tttgtttacc
     1741 agaactattt gctacaggat ataatttaga gtctttaggt ggagttaaaa cattagaatt
     1801 aataagagaa cataataagt acatagagga atcaatgtca gaagctgcta aaagaaataa
     1861 tgtatacctg atatctccat atggaacctt agaaaaaggg tctacacatg tttataattc
     1921 agcagttata tttgatagaa aaggtaagat tatgggagaa tactgtaaaa atcatctgtg
     1981 gtcattagaa gcagtatact ttaaaggagg ggaaaaagta gaggtatatg atgctgattt
     2041 tggtagattt ggtgtaatga tatgttatga tgcaggtttt ccagaagtat ccagagaatt
     2101 gacattaaaa ggttctgaaa taatatttat accatctgca tggagaatac aagatgaaga
     2161 tatgtgggat ttaaatgtat ctcaaagagc tttagagaac actgtatata cagtaggtgt
     2221 aaatttagta agtaatgact ctaatttaat actatttgga aagagtaaaa tatgcaatcc
     2281 tagaggtact gtaataactc aattagatac atacagggaa aaatatgtat tatcagagat
     2341 tgatttagat gaaataaaaa agtataggga agatataccc tatttgaaag acagaaaaag
     2401 tagctataac atataattct atactataat acaagttcaa tataaaatga taaaaagagg
     2461 atgtatcaat attaagatta aatttattat attttgatat atcctcttta atattatcaa
     2521 ctttgattta aatattttta aaatagttga taagaaagtt tttgaacaac aacacagctt
     2581 tagattcata gttttcgcta aaccatgaaa gatttaaata tcttttaaat tcaatatctt
     2641 tgatatgtaa caacttaatc tcagggtctt gagataatcc ccatgatttt ccacatataa
     2701 atccaactcc ttgaagtgat tttataagag cataaattgt atatggtgaa tcagactcaa
     2761 atgctatttt gggtttgaaa ttagctgatt cgcacaggat atcaattacc tctctataat
     2821 tctcaccctt cgttataact ataaagtttt catctgaaac ttcgcttaga tatacttcat
     2881 cctttaatga taaaggatgg tttattgaaa ctcctaatag tatttcttcg catgttaaag
     2941 ttatgctatt ttctgagttt agctttgtaa atgaagaaga tatgtacaaa tcaaaatcac
     3001 tttttttata agatgatggc aaagtatgtg acacattaaa agtaatattg ggatatagtt
     3061 ttctgaaatt actcaaaagt gatggtaaca cattagccgc tgcaggagat aatatcttta
     3121 cttctcctga attttctaaa gaagtgtctt gtaactcctt ttttgaactt tctactaaat
     3181 ctaaaatgct atctactctt tttagaaaca atcttccata ctcatttaaa acaatatatc
     3241 ttccttttct gtcaaataac tgaactccaa gttctttttc taaggaagat atagttttgc
     3301 ttaaagctgg ctgtgctata tttaacttgt gtgatgcttt tgttatgtgc tccattctag
     3361 caacagtttg aaaatactta agatgcaata aatccatagt ataaaataca cctcttttaa
     3421 tatgacttga gttatatcaa ttttataatc aactccacat tatacattat aatataaagt
     3481 ttatataatc aactacaata tattaaaaag ttatttaaat tgttatttat attttgtaca
     3541 taatctaatt aaaaaaagta gctttaaatg attaactaag gaaatgttgt tacaaacttt
     3601 agttatataa aaaattcatt tgatgagtaa agtattaatg tcaagtaaga ataaaagctt
     3661 attagataga aaaataatat tttataataa tgatgttact taaattatta tgttatacta
     3721 ttttaaatag gaaacattta gatactataa ttttaggggg agtcaagatg aaattaacta
     3781 aatcaatatc atcaatgcta tgcttagctc tagttttaag cttatcacca gtagcatatg
     3841 caaatgaaat aagtagaaca acagtggaga atactttagt aggtcaggac agataccaaa
     3901 ctgctgttga agtaagcaaa aaaggatgga gtagtgcgaa tgaggctgta gttgtaaata
     3961 gtgaatctat tgtagatgca ttagctgtaa caccatttgc taaattaaaa aatgctccta
     4021 tattattgac agaaaaaaat aatctaaatt cacaaactga gaaagaatta aaaagattag
     4081 gagttaaaaa agtttatata attggtctat ctagttctgt atcaaatgat gtgcaaaaac
     4141 agttagaatc taataattta actactgata gagttggtgg tagtgataga taccaaacag
     4201 ctttgagtat agctaaaaaa atagaaggat taaaagatat atcagaaata gctgtagtaa
     4261 atgggtacac aggtcttgca gatgcagtta gtatagcttc tgtagctgct actaatggaa
     4321 tggttatact tccagtatct gatgcatctg gtatatctag ctttaaagat tttatagaat
     4381 ctaagaatat aaataaatca tacataatag gctctactaa tgctgtatca gataaaataa
     4441 aacaaagttt acctaattca gaaagaatag gtggagcaga tagaaatgaa actaatggta
     4501 aagtaataga aaagttttat acatctaata aattaaacaa tgtatttgtg gctaaggatg
     4561 gaatgaaaaa tcaaaatcaa cttatagacg ctctagcagt aggagtactg gcatcaaaag
     4621 aaaattctcc tgtactaatc gtaggagaac aattaagtgc aacacaaaag agtgtatttt
     4681 tgaataaaaa tgctacctct ataactcaag ttggtagtgg aggaaatgaa aatgcattta
     4741 aagaattaaa agatttacag gcaaatagta acaataagag agtaatgtat gtaactaata
     4801 cagataaggt aaatataaga aatgatgcta caatagaagc ttctgttata ggatatctaa
     4861 ataatggtga tgaagtagaa gttttagatg tgttaaaaac tggatgggta aaaataaaat
     4921 acaatgaagg tattggatat gttagtgggt cttatttgac aaataataag cctgataata
     4981 gtaatgaaaa tataaaaata aagtatgtaa aagaaaaaga tggtctaaat gttaggaaag
     5041 gaccaagtac agaagatgaa aaaataggtc acctttcata tggaagcaaa gtagaaacaa
     5101 tagaaatatt tgctactgga tgggtaaaaa taaagtataa tggtggatat ggttatgtaa
     5161 gtaatgatta cttatctgat acaccagtta tttaattata agtaacaata aaaaactgct
     5221 ttaatttgta aagcagtttt tttataaaaa tttaagtcta ataactattt ctattttgat
     5281 accttaatcc tgggatttat tattatggat attatcaaca tacaaacact ccaaatccag
     5341 tgtctaattc caaacttaga tattaataaa ttcagtcctg ctatggtaaa taagcagtat
     5401 ttaattacaa ttaaaagtgt atttatagaa atccccttca tagaaagtat cctaaatcca
     5461 ataatagaca ttctaagtcc tataaatgcc aataatatac ctgcaatatc ctgaattaag
     5521 taatagtatt caaatttcat attcaatcaa ctcttctctg agcagtatca aatatttcta
     5581 actcttcaat ttcatcatct gtgtatgtaa gcatagtaaa accagttaca ccataaataa
     5641 atgaaattat aggtgtaatc caacttaaga aacaatatgg aataaattct agtgggctta
     5701 caccaagagt ctgagcacag aaaacagcat ttgaattcca tggtattaat ggaccaccaa
     5761 gtgtagctgt atcttctata attctagata gattttgtgg ttttaattta aactctttgt
     5821 atattggaga cattagagtt ccgacaaata cagaagtaaa catcattgat gaactaaatg
     5881 catttgcaaa gtagcttact ataaaagtag acccaaccaa tttagttctt gattttccta
     5941 ttttagttat aagaggttga agcagtactt ctaagaatcc agttttttgg agaataccgg
     6001 caataacgaa aactacaaat actagtaaaa cggtttcagc catactcatt attccaccac
     6061 gatttaatag tttgtcggca tagacaaatc cagtatctat agtaaatcct gatagcatgt
     6121 aatttaaaat atcacctatt ttttctcctt cttgaaatac tgctataata agtcccatga
     6181 tagctgaact tagtatagat ataatgggtg gcttttgtag caataaaagt aataataaaa
     6241 atattattgg aatcattgct actaaaccaa tatgaaagtt tgaatttaat acatccttga
     6301 tttgatttat ttgaacatag tctatagtat tatttgaata cttaaatcca attactgtat
     6361 acagaactat acatattacg tatgcaggtc ctgtggtata aaacatatgc ttcatatgtg
     6421 taattacatc tgtcttacaa actgctgcag ctaagtttgt agaatctgat agaggtgaca
     6481 tcttatctcc aaagaaagca ccagaaatga ctgcaccagc tgttaaacca agaggaatac
     6541 ccatactagt accaatactc atcattgcta taccagaagt tccaagagtc ccccaagatg
     6601 taccagtagc aacagaagtt aatgaacata cgattaatgt tgtaagcaga aaatatttag
     6661 gggttattat tgttagtcca ctatatacta ctgttggtat tgtaccagca gctatccaag
     6721 ctgctattag tacaccaaca gaaagtataa ttaagtttga ttgaaaggca tctttaccaa
     6781 cttcaaatcc aaaagcttct atttcatcat tagtataacc aagtttcgtg gctgcaggaa
     6841 ctactataag ccatgaaagt aaaaacattg tagttataga agcattaaat acaactatac
     6901 caactgatgt tagagctatt attgcaagca ttactaacaa ggaatatcta aatgaaggta
     6961 tttttctttc tgtattcatt ttcatagtgc aaattctcct taaatatttt tatttaattg
     7021 cattttttat tcttataaga gcttcttcaa gcatacttct tggacaagct aagtttattc
     7081 tttgataccc acttcctcca atgccaaagc tgtttccttg atttagagca actttacctt
     7141 tttgtacaag tacactttct agttcctcat cacttaatcc aagtgcacta aagtctaccc
     7201 ataataaata agtaccttct ggttttctaa ctttaagttt tggcatattt tcatttatat
     7261 attttatagc aaaatctata ttagattcta aatattctaa aaatgattct agccaagatt
     7321 caccattatt atatgaagct tcagttgcaa ctaagctaaa gcaattattt ctctttatat
     7381 caattctggt aaaagcatca tctaacaatt tatagtcttt ttcatcaggt agtattacat
     7441 atgatgattg aagaccagct atattaaaag ttttagttgg agccatacaa gtaattgtat
     7501 ttttttcaaa ttctttagaa atagatgcca taggaatatg cttatgtttt tttaatataa
     7561 tgtcactatg tatttcatca gatatgattt ttacattatg cttaagacaa atatctccta
     7621 gtttttttaa ttcatctttt gtccatactc taccaactgg attatgagga ttacaaagta
     7681 tgaataattt tacatcttta atcttgtttt caatgtcttc ataatccatt atatagtttc
     7741 cattttctag tttctgtaat gggcttataa ttagttctct attgttattt tttacaacac
     7801 tattaaaagg gctgtaaact ggttcttgta tcattatttt atcatttgct tttgtgagtt
     7861 catttattaa aagacttata gctgggataa cacctggact ataaattaac cactcacttt
     7921 ttattttcca gttatgtctc ctatctagcc agtttactat agattcattg taagaatctg
     7981 gtcttgttgt gtagccataa atctcttgtt ctagtctatt ttttaaggaa tcaattatac
     8041 aaggtgcagc cttaaaatcc atgtcagcca cccacatagg aagtaaatca ttagttccat
     8101 actttttttc catttctgac catttagatg aaaaattatt gcttctatca acaatctcat
     8161 taaaattata attcattgta aatacctcca gtaattttaa ttttatgtta ataatattaa
     8221 gcaaacaacg tgccaatatt gataataaaa aatatttatt cacaagttaa tatttaatat
     8281 taagtaattt cagagcatag ataagataaa aatattaatt atacttaaaa ctttgtggaa
     8341 aaggttattt ctaataaatt tttaaatata tgaaaatgta attaaatagg ttaaataggt
     8401 ttaactgttt aacttgttag acctatttat attttttaga aattctactc cttttgatgt
     8461 tataattgaa cctccacgac caacaagtat gtctacaagc ccaaagtctt gtagttctaa
     8521 aagcatattt ctaatctgtt gttctgtcaa aaatctattt tcctctaaag ctattttaca
     8581 taagcttttt ctacctattc tttcttttaa atcataagcc ttttttaaat tatccaatat
     8641 aaatatgtat tcatctagtg accttttaaa attatagtcg tatttcatga tgtttttgtc
     8701 tttgccaatg ttgaattgat acttttttat attatcactt actttattac aaacggttct
     8761 actataattt gaatctatag tgtccaatat atattcaggt aaatcacata tttctataat
     8821 atcttttccc aagtaacaga agtattctcc taagtttctt aattctctta catttccttc
     8881 ccatctatac attttaaaga tttcttttac ttcctcactt agaataaaat tacatggaat
     8941 atcatatttt aatgaaccaa aaatttcaaa tatatcagca gctctttccc taagtggatg
     9001 tattttaagg ggaagaacat tcaacctaaa atataaatct cttctaaaca tatttttaga
     9061 tacaagttct tttaaatttc tattagtagc tgcaattatt cttacatcaa tatcaatgac
     9121 attattagaa ccaatacgtc gtacttgttt ttcttgtatg actctaagta atttagattg
     9181 agaatttaaa tccatttctc caatttcgtc taaaaatata gttccattgt tggctaattc
     9241 aaataaacca attttcccac cttttttagc accagtaaaa gccccttcat catagccaaa
     9301 taattcactt tgcagtagat tttcttgaaa agtactacaa tttactgcga caaagggacc
     9361 atcttttctc ctagaagcac tatgaatgga ttgagcaaat aattctttac cagtaccact
     9421 ttcaccaatt ataaggatag atgaatttga ttgagccatt ttattagcaa ttttttttgt
     9481 attaatagtt tgtatactag aacctaatat atcatcaaat gtgtatttac tgatattacc
     9541 agagtttact agttgtgctc ttaatttagc ttgctttttt tctaattgag aaaactttgt
     9601 catctttagt ataaatccat caaatacatt taattgtata tgtttaatct ctaaatttat
     9661 atcaatatga tttattttaa tcaatttctc aaaaaatgga gttttattgg tcaatatgtt
     9721 ttgaaaattg atgtctttta tacaatcacc aacaaatgag tcaatcattt catttgcatt
     9781 tatagacaaa atttttctag ctatatgatt atagaattga attattccat cattatttgt
     9841 gcatataatt ccatcatcaa ttatagatag taacaaatca aattgtcttt caaacttatt
     9901 tgcatctaac aataactcac ctgtactata acttgtagga attatttttt ctacgtattt
     9961 ttttattagg tcatctttta ttagatgttt taatttggtt tttgtagcaa tttcaacaat
    10021 acaacttaaa tctattattc tgtattttaa atctacaact tcacaatttt taaacttagg
    10081 caggattttt tcgccaggag ttaggattac agcatctgtg ggagtaaact caatctctgg
    10141 atagcagggc aggatattta tattgtctat tcctaatcta tgtattaaag aaattgtttc
    10201 tattgccata tctttactta agttatatac ataaataatt ttatcttttt ctaatttgga
    10261 tatcatatca atactgttct tttcaaatgt caacttggga acaatgattt gagcatcata
    10321 gtgagataat tttactattt catcgtattt taaataagca gataggagta taagtttttc
    10381 tttaaaaaac tttaaatttc cctcttcaaa tgagtataaa tttatcttta gattatcacc
    10441 taagaattgc cttatttgat tttcataaat ttctccagcg tcttttttta aacttacaat
    10501 agctaattcc atttgtttca ttttaacctc tcatacaaat ttgacttatt ttagattaca
    10561 taataattaa attagattaa ataatattat ttaattatta ttataaattt attatagact
    10621 acacattgaa gtaaaagaag tagctaaaat tattcctttt atttttgttt tttgtatcta
    10681 tttggtataa tagtattaga gcgtataata atttaggcaa aattttttaa ggaagactag
    10741 aggagaatat aaaatgaaaa aatgtcttta ctgtaatagg gatataaagt caccaaataa
    10801 taaattagct ataaaatatg atgacaatat gaatatatat ccatgtcgtg cagaatgtaa
    10861 ggaaaaaatt gaagaatata tagcaaaaaa acctacatat aagtttataa atatattaga
    10921 ggttctttta ggtgtgggcg gaatattctg ctttttgagt aaatggacta ttgcagccca
    10981 agttgtactt ggaattgatg cactggttat atttttattc ccattggcag gatttaagat
    11041 gaatggaaag ttaagtatgg aacaaactaa gaatcaaagt aggtctattg caatatcaat
    11101 tttggcattt atagtaataa taaccgtatt atattttaaa caagttggta agtagagcat
    11161 aattgtataa gacatcatta gagtatgagg tgagtttatg gaagaaagaa aattagcacc
    11221 aaacaatgag agggaagctc tcataaaaag attaaaaaga atagaaggtc aagttaaggg
    11281 gatacaaaag atggtggaac aagaacgtta ttgtgttgat atattggttc aaatatctgc
    11341 tataaggtct gctataaata gagtaggcac aattatctta gagaatcata taaaaggatg
    11401 tgtagcagaa agcattaaac atgatgatgc tgataaaact gaagagatga taaaagagct
    11461 tatggatact ataaataaat ttacaaaata aggaggcggt tagttatgaa tcaacaagga
    11521 tttattatgg aaattgttga tgatagaact gcaaagttaa aaatgcaaag acattcagct
    11581 tgtgctgctt gtggaaaatg tgctactaca aattcagcag agtcaaaaga aatattagta
    11641 gaagtagaca acacaattgg tgcaaaagtt ggtgaccatg tagaagtgag tatggataat
    11701 atgaatgtat taaaagcagc tgtaatggct tatataatac ctcttatagc acttcttgta
    11761 ggaacaatag ggacttatta tatattgggg gcaattggaa tgacaactgg agttgaagcc
    11821 ataagtggat tagttggaat ttgtcttaca ataatctgtt atttatactt aaagaagaat
    11881 gacaaaaaat ttagagacag tagagaattt ataccagtaa taacaagaat attaattgac
    11941 ctttaagata attataatta aaaaatagca atgtgctttt taaataaata ctcaatacgt
    12001 atttgtagag tttaagtaca ttgctatttt tatattttta ttttgatttt ctgtaaggaa
    12061 taaagttttt tctgattttc aaaagattca atgttcttac aaaagaagtt acaactaata
    12121 caccaagtgc tattgcagca cttagaaata gcgcatctgt accttttgac atagccaaag
    12181 aataattttt ctgaatcaaa gacaacattg tattatatag cccaattcct ggtattagag
    12241 gaagaatacc tggaattacg aacacaatag ctggttgctt tagttttctt gctaaagtct
    12301 cactacacgc tgagaccaaa gcagcagcaa taaaacctgc aaaaactgca tttgtagtaa
    12361 aattaaataa ataaacatat acaatccaac caattccgcc agttactcca gctggaagaa
    12421 gtagatatag tggagcatta aagaaaacag caaatccagc agttgcaaca cctgaaaata
    12481 aaaaatgcaa aatcatagac atatcagtca ttagaaagga cctcctattc tataccatgt
    12541 atcaaggaca aaaccaactc cagaagcaat tgcaataata atcatagcag catcaaaagc
    12601 acgagaaatt cctgatatta aattccctga tattaaatct ctaatagctt ttataaaagc
    12661 aactcctggg agtagtggca ttatagcgcc gactataagc attttagggt catctaaaat
    12721 gcctatttca actagtagaa ctcctgaaag tgcaattaaa aaagaagata ctaaacagga
    12781 aaatgcagat attgcactta tcttcatcgt tttatcataa gtaattgcag caaagattgc
    12841 tattataaac gtaagtataa aatttaatac ataattccct ccaagcaaac aagcaaaaca
    12901 agcagatgca attcctgtac aagagtaaaa taaccataaa ggataaggtt ttacatcttg
    12961 gatttcataa agacgtgcaa tggcattatc aatatcatac tctttgtcac taacaaattc
    13021 tcgagaaaaa ctgtttaata aagaaatctt gtttaagttt atacctcttg attgaatcgt
    13081 tttcataaac gaaaagccat caaatttctc atctgaaata attattactg taggagttgt
    13141 aaaaatattt acatgataaa accctcttga cctgcatatt ctaaggacac tgtcttcaac
    13201 cctagatgtt tcagctccat tttttatcat cagttcccct acaaatagag ctagttttag
    13261 aatatttttc ttatattcaa gttcctcatt ttgattcata aatacacctc caaattaaaa
    13321 aacgtaatat tgaaattata tcaaattttt aagtaatatt ccttaatatc tatataaata
    13381 tattatcttt tttttgtttc aaaatagtca catttaaatt atatccaatt aaaagaatta
    13441 gacaatatta tttattatat tttaacttaa aaataaagaa agtaactatg ttaaatatgg
    13501 aaaaatggca aaacacttga ctattaattg gtaaaggact ataataataa attatcatat
    13561 tgataaaaac gaaaattatg aaatgtctgt aaaaataaaa tttaaataat aactaggagg
    13621 agtaatatgt ttgagaactt gaaaaaagcg gatcctgaga tttatgaaag tatgaaaaga
    13681 gagttaaaaa gacaacaaag aaatattgaa ttaatagcat ctgaaaacat agtatcagtt
    13741 ccagtaatgg aaactatggg aagtcatctt acaaataagt atgcagaagg ttactcaggc
    13801 aaaagatatt atggtggatg tgaatacata gatgaagttg aaactcttgc aatagataga
    13861 atcaaaaaaa tatttggagc agaacatgct aatgttcaac ctcactctgg agcaaatgca
    13921 aatattggtg tatattttgc tatgttaaaa ccaggtgatg ttgttatggg aatgaattta
    13981 tctcaaggtg gacatttaac tcatggtgct ccagttaata tttctggtca atattataaa
    14041 ttttatgaat atggtataga taaagacagt ggacttatag attttgatga agttagaaag
    14101 atagcacatg aagtaaaacc taaaatgata gttgcaggag caagtgcata tcctagagaa
    14161 atagatttta aaaagtttag agagatagct gatgaggttg gagcattgct tatggtagat
    14221 atggctcata tagcaggtct tgtggctgct ggtattcacc aaaatccatg tgaagtggct
    14281 gattttgtta caacaacaac tcacaaaact ttaagaggac ctcgtggggg tgttattctt
    14341 tgtaaagaaa aatatgctaa agacatagac aaagcaatat tccctggtat tcaaggtgga
    14401 cctcttgaac atataattgc ttctaaggca gtttgcttta aagaagcttt aagtgatgag
    14461 tttaaagaat atcaggtaca agttgctaaa aatgcaaaag ccttagctga agaacttatt
    14521 aaaagagatt ttaaattgat ttctggaggt acagataatc atttaatact attagatttg
    14581 attaataaaa atataactgg taaagcagca gaaaagagat tggatgatgc ttatataaca
    14641 gctaataaaa atacaattcc atttgatcca aatggtgctc ttgttacaag tggaataaga
    14701 cttggtactc cagctgtaac tactagaggt atgaaagaag aagatatggc aataatcgct
    14761 gaggctatag acctatgttt aacttatgat gaagaatcta aggctagaac attggttgtt
    14821 ggtttaactg aaaaatatcc tttatatgaa gaatataata ttatggatta attataaaga
    14881 acaactaaat aaaatacatt taaataatta caatgattgt gtatgtattt gaatatattt
    14941 agatataaaa aaggtgtctc aaaatgaact ttttagttta tgaggcactt ttttgtatgg
    15001 aatcgaacat atttgtgtta tttaaataat aaaaaatatg gttatctcta tttttagatg
    15061 gtctctttgt attttttaat taaaacttta agatttctta agaattatac taggtttttc
    15121 taaagattta aagtctatac ttataagtat agtaatcata tgaaaagaaa agctttaacg
    15181 aggagggaat cttatgaagg tattaattct tacaggaaaa tttggtatgg gtcattactc
    15241 agcatcaaat tctttaagtg aagatataaa agcaaaattt gataattcag aaataacaat
    15301 taaagatata tttgaataca ttatgcctaa ctattcagat aaaatgtaca agactttttc
    15361 tattcttgta aatcggggaa gtagtcttta taatttgttt tataaatgtg ctgaaaatgg
    15421 aaagaaagat ataaaattta ctttttcaga ttatttttta aataaattag atacattact
    15481 tcatgaagta caaccaacag tagttatttc aacttttcca ttttgttctc aattagtatc
    15541 aagatataag gaaaaatata actcaaatct tcctttaata acatgcataa ctgatataag
    15601 tagtcactca gaatggatta gtaaaaatac agactgttat ttagtagcta gtaaatctac
    15661 taaggaagaa cttgttttta aaggaataga tgaatctaag attaaagtta atggtattcc
    15721 agtaaaaaaa gaatttaaaa gaatagaaca tgtaaatcat tcaactaaaa aaaatattct
    15781 tataatgggt ggaggattag gattactgcc taaatcagaa caattttaca aagaattaaa
    15841 tagtttagaa ggagtaaaaa ctactgtaat aactggtaat aataaaaaaa tgtactataa
    15901 actatatggt agatatgaaa atatagaggt agtaggttac accaatgaag tatataaata
    15961 tatgaaagat tctgacctta ttatttctaa accaggtgga ataacactat ttgaaacaat
    16021 ttattcagaa cttccaatat tggcatttaa tccattttta caacaagaga tagataatgc
    16081 tagttttata ttaaataatg aaattgggag aatacttggt aaaaataaaa aatactatgt
    16141 agatgagata aaagatttaa tttatgatga tgctactctt aaagaaatga gcagtaacat
    16201 gaaagaatta aaaaaacaat ttgacaataa taccttagag aatattttat tttcacttga
    16261 tgaacaagga gcgtgtagag aatgtatgta gtaggtttaa ttatcgttgt tgcattgatt
    16321 tttttagtcc attcaattat acctacatac tataataaac tattaaataa agaggtatta
    16381 aaaaatatgg ctggagaaaa tgaaattgca cttacatttg atgatggtcc agataagaga
    16441 tatacagaaa aattactaga tgtgttaaaa gaaaatgata tacaagctat gttttttgta
    16501 gtagcaaaga atgctgaaaa agagcctgaa attataaaaa gaatgttgag agaaaatcat
    16561 atagttggtt tacattcatt ggaacacaga aatgcatggc tttatagtta tagttatgtt
    16621 aaaaaagatt ttattgaaag tacaaatata atgaaaaatt taggtgtaga tgtaaactac
    16681 tatcgtccac catggggtca tactaatata ttttcaaatt catttgttaa aaaatataat
    16741 ctaaaaatga ctttgtggga tgtaatggca gaagattggg agaaagatag tacagtagac
    16801 attattataa acaagttaat gagtagaaca aaagaaaatt ctataatttg tttacatgat
    16861 gcaggtgaaa attcaggtgg tgcagtaggt gcacctgagc gtactattga agctttaaag
    16921 atagctattc caaaacttaa agccagtggt ttaaagttcg taacaccaga aaggatgtag
    16981 ttatgataag taaaactgaa aagaaaaata aatgcctagg tagtgtagct tttttagttt
    17041 tactaatggg cataacagga tattttgttt ttagagggca gtcagttgaa tcattgataa
    17101 aatcattaaa aggtgcaagc cctatgttta tactaattgg atttgcaatg atgtttatat
    17161 atgttgcctg tgaaggtata aatatatact taggtatgaa agctttaaac caaaaaacta
    17221 ctcttttaaa atgtatggga tatgctttta ttggatttta ttttagttct attacgcctt
    17281 cagcatcagg tggtcaacca gcacaagttt attatatgaa aaaagacgat ataaatatat
    17341 catattcttc acttatacta ttagtcattg ttgttattca ccaagtagtt atactggcat
    17401 atagtggaat aatgttcatt atggaacgtg agtttatatt aaataatgta agtggtatga
    17461 acattttact aatatatggt gtaataacaa atgttgctct tgtcattgga gtaattgcaa
    17521 ttatattttc aaaaaaactt gtaaacaatt ttataatatc aataacaaat ttattaggta
    17581 agttaagaat aataaaagat gttgaaagtt caagaaaagt tataatatct caaattgaag
    17641 agtatgttaa aggtgctcaa tatataaagc agaatccaaa acttgtagtc caaattttag
    17701 tcataactat tgtacaaata acagctatgt ttttagttcc tttctttgta tacaaagctt
    17761 ttcatctatc aacatatact gtatttgaga tattagcgat acaatccttg cttaatattg
    17821 ctgtttcttc attaccattg ccaggggctg ttggagcttc tgaaaatagt tttatgacat
    17881 tatttaagat atttttccct ggtcacttat tagttccggc aatgttactt agtaggggaa
    17941 taagttttta tgcatttgta gctataagtg gattgatatg tatagtagta catgtaaaat
    18001 cttctgaaaa agtaaaagca gtgaaaaaag ttgcttatgt aagataatat tatatagatt
    18061 atttttatta atatgtcaat ttgtgtgtac attttaatta agttttaaac atgtttacag
    18121 gagtataaac tattaaaacg tgtgacattg ctaagctttt gctaataaag tataatattt
    18181 tatatagttt gaaatattta agaaatatag caaaaaaagg agcttggtga tgaataaaga
    18241 gattccaaaa aaaattttag tagctgatga taatcctgag attagggaaa tcgtagaaat
    18301 acttttaact ggtgaaggtt atgaagttat tatggcaacg aatggagaag aagctgtaaa
    18361 cttagtagat agtacgattg atttgattat tttggatgtt gtaatgcctg taaaaacagg
    18421 atttgttgca tgtaatgaaa ttagagaaaa aacgacagca ccaatattat ttttaactgc
    18481 aaaaactcaa gactctgata aagttattgg attttcagca ggaggagatg actatttatc
    18541 aaagcctttt tcatattctg aactaatttc aagggttaag tcgctactta gacgttatta
    18601 tatataccag ggaaaggaaa aggaaagaga gccaaatata ttaaaaacaa aagagttatg
    18661 tgtcaattta gatactcaag atgttacact aaatggagaa cctgtgtttt tgacaactat
    18721 agaatatagt atattaattc ttatgttgaa aaatagaaaa aaagtttttt cgtctgaaaa
    18781 tttatatgaa actatttggg atgagcagta tttttatacg gcaaataata caataatggt
    18841 tcatattaga aatttaagaa aaaagttaga aaaagaccaa aaaaatccac aatatataaa
    18901 aaccgcatgg ggaaaggggt attatattga ttaacaaaat taagccatct aagttgggag
    18961 ttatactatt gatatatgta gtaatctcag ctgcagtggc tttcatttta aatataccat
    19021 tttactctgc tgttgaatct atagtaaaag atgtatcaga agggaaagta tattttaaaa
    19081 gagaagtaaa tcaacttatg gataattttc aaacttatgt gaataacaat ggtataagag
    19141 cagatgatgg agatgatata gcaaaatgga atagtgataa ttggtttgtt tctatgaaga
    19201 tatataaagg aaataaggtt ttttatgata cattatacta tcctaatgaa gtacctgata
    19261 agagtgagga aaatgagttt tatagcagtc ccatggctag aaactatgag atacaatttt
    19321 ctgatattaa agctaatata gttgtagtag ctttatttag atttagattt caaaatttta
    19381 tagaagcaat ttctattaca tttatggcac ttacatttat tataacattt ttactgttgt
    19441 ttagtagaaa gataaagtac ctaaggaaaa tagaaatggg tataaagata gtagagagtg
    19501 gcagtttaaa gtatagaatc ccagtaaaag gtaatgatga attatcatca cttgcatcta
    19561 gtattaatga gatgagtcat acattggaga atcaacttat aaaagaaagg gagatgaaga
    19621 ataaaaataa agatatagta acttcgatat cacatgatgt tagaactcca ttaacatcag
    19681 taatttgtta tttagatttg atcaaagacc ataaatacaa tagtcctgag caattggaac
    19741 aatacataaa aaatgtaagg tctaaagcat atcaaataaa agatttaaca gatgaccttt
    19801 ttacgcattt tgttaattct ggagaggaag tttcaaaagg gtatgaaaaa ataaatgcta
    19861 atgaatttat ttttcaatta cttttagata gttcatatac attggaggag aaaggatttc
    19921 atataacaat agaaaatgaa ctttataacc aatatcatat ttatgtagat gttacacaat
    19981 ttagaaggat atttgataat ttatgttcta atataataaa atatgctagg aaagataagc
    20041 ctatagtatt tttaattaaa gtagaggatt ctaaattact aattatacaa aaaaatagag
    20101 taagtaattt ttctaagaat gtagaaagtt ttggaattgg tataaagaat tgtgagaaaa
    20161 tagttgcata tcataatgga agcataaatg taaattcaga tgataaagaa tttgctattg
    20221 aaatatctat tccagcaaaa gaatcataaa aatttaaatt atatattgtg taattgctat
    20281 tatatattgt gtaattgctt atgtaaaatt ctatgaagca ggaagcatgt gactttagtt
    20341 atgggatgtt caatattgtg tacctctagg caagtaacaa gtttacttac aagaccatat
    20401 aatattatta tcttaaaaaa aggggacata ataatatggt tttgacagga gcacattata
    20461 tttacattct atttatggta attatattaa ttactatgat tatgaaaaag gatacaatag
    20521 taccatgtat attaggtgtt ttttttatgg gattgttctt tgaaaaaaac atctttggtg
    20581 ctataacagc agtgtttaac tcttttatta tatctttaaa tgagttgggt ccaattatct
    20641 tgattatagc tattatggtt gcattatcta aagccctaga agccaataat gctatacagt
    20701 atatggtgag accattttcg agagttataa aaaattctaa tacagcattt tttgtaactg
    20761 gatttgttat gttagttctt tcttggtttt tctggccaac tccagctgtt gccttagttg
    20821 gagctgtatt tttacctgtt gctatgagag ctgggctacc agcaattggt gttgcagtag
    20881 cattaaattt atttggtcat ggtcttgcct tgtctacaga ctttgttatt caaggagctc
    20941 caagtataac agctggtgca gcaggtgttg cagtatcata tgttataaat gatggtatga
    21001 ttttgttttg ggtgatggga atcgtaacca tatctatggc attttataca ttaaaaagag
    21061 atattaacaa aggaatgttt agagaagaac taaaaggttt tgaaagtgaa gaagtagaag
    21121 agttcaatgg aaaatctaaa atagcaacta ttttagtagc tctgggattt cttgctgata
    21181 taattgttat gtatatattt gacctaaaag gaggagatgc atcagctctt ttgggtggaa
    21241 cagcagtatt tttaataata attataaatg ttataaattt tggcaaagat agtcttgaaa
    21301 atgtatgtga gaatataatt gatggttttg tgtttgggat aaaaatattt ggagcaatta
    21361 taccaatagc agcatttttc tatatgggtg aagttgcgcc cttgacaggt gtatttggaa
    21421 aagtattagc accaggttca caaggtttat tatcagatat aggcatagct ctttctcaaa
    21481 caataccact taataaattt gccgtatcag gtatagaaac tgtagtgggg gcaataactg
    21541 ggcttgatgg ttctggattt tctgggattt cattagttgg ttcacttgcc tctgtatttg
    21601 gaactgctat aaatgcaagt gttggtgcat tagctgcatt aggacaaatc agtggtatat
    21661 gggtaggtgg aggctgtcta gttccttggg gtcttatttc agcagcggca atttgtgggg
    21721 ttagtccaat agagttggca aagcgaaact ttgtcccagt tataaccgga ttggttgtaa
    21781 caaccatagt agcaatgttt attatttaat aaatcgaata aatctttaat tgtacaaatt
    21841 aaaaatagat gtgtagctat aatttattat aattgtttta ctaattttaa gccatacatc
    21901 tatttttatt gatattttta ttagtattct ttaataaaat aatatttttc attatactat
    21961 acataacatc taaatataac atctaaaata ctgttatcta tgtttaatat cttgccaaat
    22021 tttttgatgt tttattaccc tgtcccacca acatatataa gctatcgcaa gttttttacc
    22081 tatactatca ccaaacaaaa attttaattt taatttatta ttttataatc tatattatat
    22141 taactatatt agtttgtaga ctttagatgt aattctaaat attaatactc caagagttca
    22201 aattcatatc ctagttttct tgcatttgat ataaaatcat ttaaaatttc tgtactagtt
    22261 ttggaaactg catgtagtaa caagatagaa ccatcatgta gattatccat tattttttgc
    22321 gttgcttctt catgtgatgg ttgtttatca gtatcccagt ctctgtatgc aaaactccaa
    22381 aatacagtct tgtatcctaa atttttagtc attgccaagc ttttttctga gtattttccc
    22441 ataggaggtc taaaaaattt aaccatatct ttcccagtta catctttata taacttttca
    22501 acatcatata attcgtcatt aaagtttttt agattgcttg tttttgttgg catagatgga
    22561 tgggttttac tatgatttcc aactatatgt ccctcagaaa ccattctttt tactaaatct
    22621 ttattttctt ttatatatgg agctgtaaca aaaaaaactg ctttaacctg gttttgtttt
    22681 agagtgtcga gtatctttgt agtgtatcca ttttcataac cttcatcaaa tgtaagatat
    22741 agtgtttttt gaccatcttt tgtaggtcca ttgtataggg catcatagtc agaaaattta
    22801 aattcaatgt ccgtatttac ttctggtgtt ttatgcttat tgttaggtat ataaaaccaa
    22861 tcaagagtag tgttatctaa agaagataca tctaatttat tattatcatt attagtagtg
    22921 gcttttgatg gtttatctgt tccgtctgaa actattacat tggaagtatc cttaccacca
    22981 tctatttttt ccttatcttc ttgtaactgt gtttccttat tttgattttc gttttgattg
    23041 ttttgtgaat ttgaacatcc aacaataaaa aaaatagaaa tagaaagtag tgttaaataa
    23101 tgcaatactt ttctaaacat tttgaagctc ctttctaaat taataataat tgcatatagt
    23161 attattataa ttggaaatta ctattaataa tagttatatc tttaaaaata aagtcacaat
    23221 agtatgacaa agggtgaata atcaataaat ttaaaaaaaa atgaaaaaaa tagttgacca
    23281 tattatgaga agatgttatt ataataaatg tgctcaacaa gaagtaacaa aataacaagc
    23341 acaaagaagt taattgaatg aaa
//
LOCUS       DAEPLF010000039        22778 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEPLF010000039 DAEPLF010000000
VERSION     DAEPLF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.039"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            147..983
                     /locus_tag="KR314_001368"
     CDS             147..983
                     /locus_tag="KR314_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0327759.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFKFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1336..2913
                     /locus_tag="KR314_001369"
     CDS             1336..2913
                     /locus_tag="KR314_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0327760.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2906..4090
                     /locus_tag="KR314_001370"
     CDS             2906..4090
                     /locus_tag="KR314_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0327761.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     TL"
     gene            4108..4707
                     /locus_tag="KR314_001371"
     CDS             4108..4707
                     /locus_tag="KR314_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0327762.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4731..5201
                     /locus_tag="KR314_001372"
     CDS             4731..5201
                     /locus_tag="KR314_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0327763.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIELKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            5558..6304
                     /locus_tag="KR314_001373"
     CDS             5558..6304
                     /locus_tag="KR314_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBH0327764.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            6366..7040
                     /locus_tag="KR314_001374"
     CDS             6366..7040
                     /locus_tag="KR314_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0327765.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCLNCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCALRCMMSCPWSL
                     RYANQK"
     gene            7408..8754
                     /locus_tag="KR314_001375"
     CDS             7408..8754
                     /locus_tag="KR314_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBH0327766.1"
                     /translation="MEIETKTPDWINVLELNSKIDITWKYDVSNMIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIVGTTNFITRGVSNI
                     SIITLEGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLFHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     REIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITKCGQYYKSVDYNTYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNKVMLTC
                     YAEADELIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSIEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            8781..10181
                     /locus_tag="KR314_001376"
     CDS             8781..10181
                     /locus_tag="KR314_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBH0327767.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSSKLQEIIDAAPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GEPGHYENLFFSGFSGIALDMPNNSTANDITVFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKVIKMHKPEVGDIMSTIIKDENILSAHYGGTWGKIGE
                     KIEFGITVHYYKLIEK"
     gene            10391..11827
                     /locus_tag="KR314_001377"
     CDS             10391..11827
                     /locus_tag="KR314_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBH0327768.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPDLQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSPTETTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNEEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKDYEGYGSYKINNITIDQCGYCGFLFDSVKGSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGKETVYYYKHHEPDKTNQIK"
     gene            12171..13115
                     /locus_tag="KR314_001378"
     CDS             12171..13115
                     /locus_tag="KR314_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBH0327769.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            13284..14216
                     /locus_tag="KR314_001379"
     CDS             13284..14216
                     /locus_tag="KR314_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBH0327770.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            14321..14788
                     /locus_tag="KR314_001380"
     CDS             14321..14788
                     /locus_tag="KR314_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBH0327771.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGETMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(15032..15886)
                     /locus_tag="KR314_001381"
     CDS             complement(15032..15886)
                     /locus_tag="KR314_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBH0327772.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            16052..18016
                     /locus_tag="KR314_001382"
     CDS             16052..18016
                     /locus_tag="KR314_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0327773.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLSEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSCCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFVKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLLEESI"
     gene            18016..18477
                     /locus_tag="KR314_001383"
     CDS             18016..18477
                     /locus_tag="KR314_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0327774.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSDAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            18480..18800
                     /locus_tag="KR314_001384"
     CDS             18480..18800
                     /locus_tag="KR314_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBH0327775.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            18816..19916
                     /locus_tag="KR314_001385"
     CDS             18816..19916
                     /locus_tag="KR314_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBH0327776.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            20036..22636
                     /locus_tag="KR314_001386"
     CDS             20036..22636
                     /locus_tag="KR314_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBH0327777.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKHVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDYILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNVFIEESGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLILKDSGNAGDTYDFSPLKNDEDIKLSFDRVYTEKTLGYEKLVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPEKY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         8942 a         2354 c         4025 g         7457 t
ORIGIN      
        1 atgacaataa aaaatatttt gttattgtag tattataggg taaattgcaa aatatatacg
       61 aaagtaaaat tttaaatatt gacattaaat taaatatact atactatgta agtgtgatga
      121 gagaattttt ttagagggga tttatagtga atataaaaaa gatatttaat aacaatgtag
      181 tagtaagctc cctagaagat ggcacagaaa taattgtcac tggtgctgga gtaggattta
      241 agaaaaaagt aggcgatttg atagatgaaa atttgatttc taagaaatat tttgttcaag
      301 acgatcaaag ggacaaatac aatcaaatac ttaataagac ctctattgaa tactttaaaa
      361 tttcagaaga aattatagaa aaggctaatg aagttttaaa tactcaagtg aatgattcaa
      421 taatattagc cttaactagt cacatagaat ttgctgtcca aagagaaaaa caaggtataa
      481 aattaccaaa cttaatattg aatgagacaa aacaacttta tagggaagaa tttaaatttg
      541 gattatgggc aattgatgaa attgaaaaaa ggataggaat aaaattgcct gaagatgaag
      601 caggatatat tgctattcac ataatcaatg gtttggaaaa ttctcaaaaa gatgaaggta
      661 ttaatatact agagttttca aaacaagtta ttcagataat tgaagaagta catggattta
      721 aattggatgt aaattcatta aattatacta gacttataac tcacttaaag ttttttgttc
      781 aaaggattct ccgaaaagaa acttacaatg atagtgatat tgaagatatg tataagttag
      841 tgaataaaaa ttacaataaa cctaaagtgt gtactgaaga aattgcagca ttggtattag
      901 acagatttga atataagatt agtaaagaag aagaattata tttaatgatt cacataaata
      961 aaataacaaa cagtggttgt taggattgta actgataaag caggcaaaac ctaagtcaat
     1021 gagcttaact attagattaa gttttttgat ttaggttttt ttattagata caatcaaaat
     1081 acagacagtg acttttagga ttgtaactgg ttaagcaggc aaaacctaaa tcattgaatt
     1141 taactattag gttaagtttt ttgatttagg tttttttatt agatacaatc aaatacagac
     1201 agtgactttt aggattgtaa ctggttgagc aggcaaaacc taaatcatta aacttaatta
     1261 ttaaattaag ttttttgatt taggtttttc tataaatttg attatgtttt tagataaaaa
     1321 taggagggga aaattatgaa aaaaaataag ttaggtttat ggaacttctt ccaaatgtta
     1381 gggaaaacgt ttatgttccc aatagcacta cttagtgttt ctgggatgat gctaggactt
     1441 ggggcaggtt ttacagaccc taagatgatt gaaatggttc catttctagg gaatgaatat
     1501 gttaacttag ttttaaactt catgttaaca ataggattat ttgcatttaa caacttggga
     1561 gcattatttg caatggcaat tccactagga ttattaaaaa aagaaaaaga gtttggtgct
     1621 ttttcaggat tagtaggatt tatagcaatg catataggta caaattttta tttaacaaga
     1681 gctgatttgt tagtaccagc agaacagatg tcaactaatg gtcaagctat gattatgggt
     1741 atacaaacat ataatacaag tgttttaggt ggtattgtag caggattaat tgtttatgca
     1801 atatatgata aagttagtaa tttaaagata ccagaatcat taggattcta cagtggacca
     1861 agattagttc caattgttag tttaatagtt atgagtatag ttggattatt aatacctatt
     1921 gtttggccac catttttcaa tgcattccaa agtttaggaa gatggatttc ttcagcagga
     1981 ccattaggat atttttcata tgcagtggca gaaagagtta caattccatt tggactaaat
     2041 catcttgtaa catctgtatt ccgttttaca ccaataggtg gaactgctgt tattgatgga
     2101 gaaacgtact ttggtacatt gaacatgttt atggcatatg tagaaaataa tcagattata
     2161 cctcttgatt tagctggtaa aatggaacaa ggtaaattga tgattcaata tggattagct
     2221 ggtgctgcat tggctatcta ccgtacagca aaaccagaaa atagaaaggc acttaaagga
     2281 cttttaatat caggagtgtt gacagttatt ataggtggta taagtgaacc aatagagttt
     2341 ctattcttat tcatatcacc agcattattt gctttccata catttatgaa tggtctagca
     2401 aacatggtac ttccatactt aggcgtgttg atgggattca caggagattt aattgcattt
     2461 ataagttttg gtgtgttaag aggaacagct acaggatggc caatagcagt attagtagca
     2521 gttttatatt ttgcaatata ctattttgta tttaagtggg caattattaa gtttaatata
     2581 aagaccccag gacgtgagga taaagaaata aaagtatctg aaggaaatgg ttcagatttc
     2641 aaaaatttgt ctacatataa aggacaacaa atgatatctg ctttaggtgg tcaatctaat
     2701 atattatcac tagataattg tgtaactaga ttgcgtttaa aactacaaga tgtatcatta
     2761 ataaatgagg atgcaattaa agaggctggt ggtatagcag ttgttaaatt ggatgagcat
     2821 acaatacaag taattatagg aactcaagtg tattctttaa gaaagcagat ggataaagct
     2881 atggaaggtc atttggaggc atgtaatgat taattttgat aaaaaagtag accgattggg
     2941 tacatattgt acccagtggg actatgtaga agatagattt ggtaagaagg atttgcttcc
     3001 ttttacaatt tcagatatgg atttagagtt tccatttgaa ataacagagg tgttgcagga
     3061 aaggttaaat catagagttt tgggatacag tagatggaaa catgatgatt ttagaaattc
     3121 agttacgaat tggtatatta aaagatttaa ttgtagcgtt gatagtaact ggatatatta
     3181 tagtccaagt gtaatgtatt caattagcaa acttattgag atgttaagta atgaaggaga
     3241 tggaatcctt ataaatactc ctgcatacaa tgctttttat gatgtgataa caaataacaa
     3301 tcgtaatatt ataaagtcac atcttgtaaa taggaatgga ttctattcaa ttgattttaa
     3361 tgattttgag aaaaagtgta aaaaatctaa gatatttatt ttatgtaatc ctcacaatcc
     3421 aactggtaga gtatggagtg agttagaact tgttaagatg ataggaattt gtaagaataa
     3481 tgatgtcaag ataatatcag atgatataca catggatatt gtatatgaca atagaatgac
     3541 acctatctta aatgttgcaa aggactattt agattctgta ttcatttgta catcagcgtc
     3601 taaatctttc aatataccag cattgactgg gtcatatgtg attataccaa atgaaggtat
     3661 tagaaataaa tttgaaaata tcactagata ccgtgatttt gtaaattcgc cagcaattct
     3721 agcaatatta gctacaatgg tctgttatga tgactgcgaa tattggttag atgaactatt
     3781 aaaatacctt aaagagaatc ttaaatatac gattgattat ataaaagata atttagaacc
     3841 attgaaattg gtcatgccag aaggctgtta ctttgcttgg atagattttt ccgaacttaa
     3901 aataagtggt gagaaattcc aaaaattatt gatggatatt ggagaagttg ctattatgtc
     3961 ggggtctgta tatggtgaag aaggtgagtt ttatttaagg ttaaatgtgg catgttcaag
     4021 agaaaaactt gaagatggtt taaataggat taaaaaaact atggattata ttgcaaaaag
     4081 aactttatag taggaaggga gatagttatg ggatatttaa aaacaatggt tgatgttatg
     4141 actactgaaa tgaatgccat aagatgttta atagatgaag caggaataga atatgaaagt
     4201 atagttagtg aaattgcaaa ttgtgaaggc aaagtaattt ttatgggtgt aggaaaatct
     4261 gcacatatag gtaagaaatt ggcagctaca tttgctagta ctggtacacc tagctttttt
     4321 gtacatgcaa cagaggcagt gcatggagat ttaggaatga ttgaaagtaa ggatattaca
     4381 attttaatat caaatagtgg aaattctatg gaagtggtaa actgtataaa atatattaaa
     4441 gcaataggtt ctaagacaat tgcatttact tcaaacagaa attcagtact ggcaaaagag
     4501 tgtgattatg ctttaatata tcctgcaaag gatgaagcag accatttaaa tctagcacca
     4561 acaacatcat ctacaattac tttggtttta ggagattcta ttgcatgtgc attatctaag
     4621 agtagtaatt ttggttcttc agatttttat aagtaccatc caggaggaag tttaggtgag
     4681 aagttaaaaa cagctaacaa tggataaaag caaaaaggag agtagttttt atgttcaaaa
     4741 tgtttaaaaa aaaatcaata aaatcaccca tatcaggaaa agtaatagag ttatctaaaa
     4801 tacaagatgg tgtattttct caaaaaatta tgggagaggg tgtagctata gattctacag
     4861 gagacattgt atatgctcca atcaatggaa agatagtagt agtagcagag actaaacatg
     4921 catttgtaat agaattagaa aatgggatgg aacttcttat tcatgttggt ttagatactg
     4981 taaatttaaa aggcgaaggt tttgaggcat tggtgagtat gggagaacaa gtgaaggaag
     5041 gaacaccaat gttgaaaatt aaccgtcctt tgattgaatt aaagggtata tcacttatta
     5101 ctccagttac aataactaat cattcagagt acaatatgaa tacatgtaat gtaggcaata
     5161 gcgttgaagg tgggaaagat actgtaattg agtttaaata aagaattaaa atacaaggtt
     5221 aatatttata aatacctata aacttatgtt ttataagatt gattgtatgg atttattgta
     5281 taaaaaagaa ctctttaatt ataaattaag gagttttttt attttgtaaa tattgtttag
     5341 atttatatag aaaaacactt agtaataaat agaataaata agtatttata tagctaaaaa
     5401 tctaaatagt tttgtttaaa tatattttta tattttgtaa tttcaaatta ataaataatt
     5461 agaattgata aacaaaaaat aaaaatgttg attatattaa taaactttaa tataatatat
     5521 ttatgaaaaa tgtaaattgt gaaatggagg tagtaacatg aaaattgaaa atctttctat
     5581 tgatgatatt aaacgaggct atatatttaa tgatgattta aattgttata gttgcattaa
     5641 atgtacaaaa acatatgagg taggagaaat atataaaata ggtaatagat tctttgaagc
     5701 ttcaaaagct attgaacttc atatggaatt agaacatagt gattatttag agcaattgct
     5761 taatagtgat tcaaagtaca acacattgac tgaaaatcaa aaacaattat ttcaattatt
     5821 tattagtgat ttaccagata aggagatagc aaaacaggtt ggagtatcca catctactat
     5881 acgccatcaa aaatttattt tcagagaaaa agcaaaacag gctaagctct atttagcact
     5941 atatgaatct gtatttgaag ataaatctaa taaaagtagt actataatac caatccatga
     6001 acatgctcga atggtagatg aaagatatgt agtaactgaa gaagagagaa aacacatttt
     6061 ggatacatca tttgaaagta tgaatccttt aaaattaaaa gtattttctt caaaagaaaa
     6121 gaaaaaggtc gtaattttat ctaagatttt ggaacaattt gaacgtggga agaaatattc
     6181 agaaaaggaa gttaatcaga ttttagaatc tatatatgat gattttgcaa caattagaag
     6241 atatttaatt gagtatggct ttatgagtag aaataaagaa tgtacaaagt attggttaac
     6301 ataattaaaa taataaattt tactaatgtg taatgttatt tttatctagg ggggaggagt
     6361 taaatatgaa tgatgaatta aaatctattt tatacaataa aggtgtagac attgttcgct
     6421 ttgttgatat atctgaattt ccaataaatc aaacacaagg tttttctaaa gcaattttat
     6481 tttgtatagg gttatcaaaa aaatttatta cagatatata caataactta cctacagata
     6541 gtgatgagtt tttggaaaaa gaggagaaag tagaagaatt agctgactgg atttcaaaat
     6601 atattcaaaa taaaggatat agagcgtatt ctcaatcgga gaaaaacaat ctagaacatg
     6661 gatattttga aaaagcctat attaatccag aaatgcaatc agggattagt cctttaccac
     6721 ataagacaat agcaaatatt agtggcattg gatttatggg aaaaaataat ttgtttgtaa
     6781 cagaggaata cggatgtgct tttagcatgt gtacagtact tacagatgct ccaatttctg
     6841 ttgaaagata tcctttgatt gattcaaaat gtttaaattg taatgtttgt gtagagaact
     6901 gtcctgcaaa agctatacat ggaaatgaat ggactttacc tggaaaaaga gaatctatta
     6961 ttgatgtttc aaagtgtttt tgtgcattga ggtgtatgat gagttgccca tggtcattaa
     7021 gatatgcaaa tcaaaaataa ctttactaga gaaggtatta aattgaatat tataaaattt
     7081 ttaaaatgta agattatata agatttctca aaataaaaat ttgtatagaa cactcagaaa
     7141 tgagtgtttt tctattggga ttaaattata ttgatacaaa gcttaactat tttaatcatt
     7201 atataaaatt tgtattaaaa aattaagaat aacttgctaa tagtaaaaat ataatgagat
     7261 tataattgat ttataatata actgtaatga tgttgtaaaa tgaaaattgt catctcaaaa
     7321 ttcccaaaaa gagtaaaagt cacttcggtt atagtcaaaa aattttttct gtgaaaagaa
     7381 tttagattta aaagggagga attaatcatg gaaattgaaa ctaaaactcc agattggatt
     7441 aatgtactag aattaaacag taaaatagat attacttgga aatatgatgt aagtaatatg
     7501 atacaagata ttgtctctga taagtcacta gaaggttcca ttatatattt tccaaaaggt
     7561 aattacttat ttgagaaagg tattaaaatt agtcaacaaa taacacttca aggagattct
     7621 tattatggag gagataatca agtatcaaat ttagataaaa aacaatcaat cgtaggaaca
     7681 accaatttta taacccgagg agtttctaat ataagtatta ttacattgga aggaacaagt
     7741 caatgtatca aaaatattaa tttttattat gatagtcatg atatagagaa aataccacct
     7801 aaaaatgtat ctgctattac agaatatgga gaaacacaag gactatttca ctttgaacac
     7861 ctgtttattt ctggattttc aggaataggg attgaaattc catactattc aactggcaat
     7921 gatatcattg ttagttcatg tggtcttggt atgaggcttg gagagaagtc aatgctttct
     7981 agtagcaaga tttatgaatg taaaaatgga atggagatta ctactggtgt tagcttaaat
     8041 aatgtgagaa tagaagaaat aagagaaata ggtattaata ataaaggatt tggttttaac
     8101 ctgattatga atcttatggt cgaccaatgt ggatattgtg gatttatgtt tgaaaaaatg
     8161 tgttatagcc agataactgc cagaattact aaatgcggtc agtattataa aagtgttgat
     8221 tataatactt atgagaatat ggaagataga aaagaagaag cttattccat tttttatgga
     8281 gatattttag aaaattgcaa tattgtattg atgaatagta atattgatac cttagaaaat
     8341 gattcacttg atgaacataa gatatatgta atcaaggcaa atgaaacaaa caaagtaatg
     8401 ttaacatgtt atgctgaagc agatgagctt atacagtgcg caaagggcaa cttgttgcta
     8461 gaaaatggtg aaaatactta taaattttat aatggtgaga tttgttctgt aggaggtgtt
     8521 caaatttctg atatagatga aaaagattta atcaaaataa tcgagaacac tctgtatata
     8581 gatgataaag gtaagtactt aatggttcct agattaaaaa agaatactgt aatatattca
     8641 gtactagatt ctatagaaga aataaataaa ttacatggtg gaatatggga acagattgat
     8701 gtaaaagtat tagctgggga atctatgtac tattataaaa agataaaaga ttaaattatc
     8761 aaactaggag gtagtgatta atggaagagt ctataataat taaagatata agtggctgga
     8821 ttaatgtact tgattatggg atttgtaatg atgaaacaaa agatgtaagt tccaagttgc
     8881 aagagattat agatgctgca ccagaagatt ctattatata ttttccaaaa ggaaaatact
     8941 tatttgaaag tggtgttaag attaagaaac gattaacctt atgtggagat tcttatattt
     9001 tggcgaatgt tcctaaaaac actgctggag taacacaatt tacttttaaa ggaaagaaaa
     9061 atcctaaaag tgaaaatata gcaattataa cagcaaaaag tgtaagacac tgtattaaga
     9121 gcatttgctt ttattcagat agttgtgata gagatatgac atctaatcat gaacctccaa
     9181 cagatggaaa acctagattt catcataatg taattataaa ttatagaaat atatcggcta
     9241 ttgtatgtga agataaacag ggagaaccag gtcattatga aaacttgttt tttagtggtt
     9301 tttctggaat tgcacttgat atgcctaata attctacagc aaatgatatt acagttttta
     9361 catccggact tggaattagt acaggggaaa attcaacgat tgctaacagt aaggtttggg
     9421 gctgtgttga tggaatgata atttctactg gaacttttat aaatggtatg agggtagaag
     9481 aaataaaaaa agttggaatt aaaaatattg gtagaggttt aaattttatt acaaatatca
     9541 caattgacca atgtggatat tgtggagttt ggtttgattc tatatccaat gttcagatat
     9601 caggaaatat cacaaggtgc ggacagtact attatgatgt aagctatgat aattatttaa
     9661 tgttggataa tagagttaaa gaagcatatt ctttattcta tggtaattta ttggagtcaa
     9721 gtaatatagt tttgtcaaat accaataaag ataattggga agatttcaaa gaggataagc
     9781 ataaggtgta tcttgtagaa gcacttgaaa ccaagaatgt actattaaca tgcaatatag
     9841 atgctactga ctggattgtt aagtcagaaa aaggaaattt aacgtacaat aatatgcgac
     9901 aaactgttat attttacgat ggaaaactaa gtagtatcaa tggaattggt atatctgatg
     9961 aagattatgg ggataaagtc aagataaaaa aaggaaatat atatataaat agtaaggata
    10021 aggttattaa gatgcataaa ccagaggtag gtgacattat gtcaacaatt attaaagatg
    10081 aaaatatact ttccgctcat tatgggggaa catgggggaa aattggtgaa aaaatagagt
    10141 ttggaataac tgtacactat tataaattga tagagaaata agtaaatacg tagtatttga
    10201 atagatgtat tttattggag agctaggact atagttttaa tgtaaatggt ttggtaatag
    10261 catggattga ataatataaa aataatataa aaaattaatt tgattatgta aaattctatg
    10321 aagtagtaaa cctgtgactt tagtcatggt aggttcaaat taagtaaatg ggaaaggaat
    10381 atataatatt atggaaggaa gaactgggat taaagatgga agatggatta atgtagtaga
    10441 aagaggtatt aaaaatgatg gttcgtacaa cataggtcct gatttacagg atattattga
    10501 tgaaatagaa acagatttct gtgtcttata ttttccaaaa ggaaaatact tatttacaag
    10561 tggtgttaaa attaataaag aaattgtatt acaaggtgat tctaattcac ctacagagac
    10621 tacacaattt ataactcgtg gagtagaaaa tatgagtatt attacattga cagggaagaa
    10681 acaatgtatt aaaaatataa atttctattc tgatagttgt gagatacagg taaatgaaga
    10741 accaccgaca aaaggtaatc ctaaatatca ttatgagatg attattaata agaatgaaaa
    10801 aaatgttgag atgaataatg tatcagcaat tctgtatgat aatagtaaga atatgattaa
    10861 aggtctaggt cattatgaaa atatttatat aagtggtttt tcgggtacag gaattaggat
    10921 accatattac tcaattgtaa atgatattac agtttcatct tgtgggttgg gtattgatac
    10981 aggaatagac acaattattt ctaatagtag aatatctaag tgtcaaaatg gaatgaggat
    11041 tagaactgga actagcataa ataatgtaag aattgaaaaa atacagaaag ttggtcttat
    11101 aagtagtcaa gttaaggatt atgagggata tggaagttat aaaattaata atattacgat
    11161 tgaccaatgt gggtattgtg gttttttatt tgactcagtg aaaggtagtt atttttctgg
    11221 tattataaga agatgtgggc agtattatta taatacagat tatgacacat atttaagtat
    11281 taaagataga gtagaagaag catattcttt attttatgga aattatttta cagaatgtaa
    11341 tattgattta gttagtgata ataaggataa ctgggatgat ggcttagaat ataaacataa
    11401 agtttatgta tttaaaaccc ttcaaatacc aaaattaaca ttgagatgca atatagatgc
    11461 ctatgattat atcgttaaat cggtacatgg aggaggtaat ttaatgttaa aaaataactt
    11521 taacaattat atatttattg attcatctag tccagaagtt attaatggaa ttaattttaa
    11581 ttatcaaaag aatggaaacc tgttaaggtt aaataatggt aaattaagtt ataaaaatgg
    11641 aaaggaagaa acagctatat tcagacctaa ttatggtctt attgtagcat caacagattc
    11701 agaatgtgaa caaatagaga ctagctatgg aggaaaatgg gagaagcttg gttctgaagt
    11761 aattggaaaa gaaacggtat attattataa acatcatgaa ccagataaaa caaaccaaat
    11821 aaaataaaat ttgtattata taatgatata aagttttaat atatagtgtt tacctttaat
    11881 gtaatattgg tatatttgta tagtattaaa tttgttaaaa agtaaaattg tagggtgctt
    11941 gaaaatagca ccttttttag tttcattata aaattagttt tgaaagttta ggaataatgt
    12001 atatttattt ttaaagaatt ataaccttat taaggatatt ttaaatgcta gaaatgaatc
    12061 agtaaatcat actggaatta tcttaaatgt tttgattaaa ttaagtattt agagctataa
    12121 ttatattaaa aatatattag gacataatta tattactatg gaggttaaat atgatagtaa
    12181 gtgtgagtag aagaactgat atacctgctt tctatagtaa gtggtttttt aacagactta
    12241 aagaaggttt tgtttatgta attaatccaa tgaatccaaa acaggttagt aagatagagt
    12301 taaatccaca tactgttgat tgttttgtat tttggacaaa ggatgtaact ccaatgttat
    12361 atgatttaaa taaattaaaa gactataaat attattttca ctatactata acatcatatg
    12421 gaaaagaagt agagacatgt attttagata aaagaaaggt aatagattca tttaaggaat
    12481 tgtctagaaa aataggcaaa gaaagagtaa ttttaagata cgacccgatt ttcttaagtg
    12541 aaaagtacac agtagattat catatcaaag catttgcgag tttatgtaat cagcttgatg
    12601 gatttacaga aaaatgtata ataagtttta tcgatttata taagaaaaca aaatttaata
    12661 ctaaagcatt acatataaag ccaatagaaa ttacagaaat agaaacatta tcaaaagaaa
    12721 tttctaagat tgctcataag catgggataa tacttgaaat gtgttctgaa gaatataatt
    12781 taagtaaatt tgggattgga aaaagtaagt gtattgatga tagattaata tcaaaaatta
    12841 taggatgtga agtagaggta aaaaaagata gtacacaaag agatatttgt ggatgtgtta
    12901 aaagtgtcga tataggacaa tacaatactt gcagacatta ttgtctgtat tgttatgcta
    12961 actttaatta tgcaaaagta gaagaaaact gtaggcttta taaagaagat aataaattac
    13021 tcatagatac tttaagagat gatgcaaaaa taagcattag agatatgaag actattaaag
    13081 tagataagaa taaacaaatt agtatatttg attaaaataa atatactaat taccttaata
    13141 tatcaagaat atagatactt ttgataacaa aattaaactt aatagatatt ttctatataa
    13201 gaaagatggt cataatatgt caatttgacc atctttttga tataattaaa acacaaataa
    13261 tttagtaaca aaggaggcag aacttgaatt tattaaaaaa atatcctata cctattgcaa
    13321 gtttgatttt gtcaattttt actttaggta acttattaca atcttatagt gaatcattac
    13381 gtaatgtaat tggatttata ggatttattt tgtatgcaat ttatatagtc aaaataatat
    13441 tacttagaaa gggtgttaaa gagcaattag agaacccttt aatatcaagt acatttccaa
    13501 ctataacaat ggcaacaatg atatttgcta catatataaa accattatct ttagaattaa
    13561 gtattatgat ttggagcata ggaattgtgg gtcatcttgt gttaatagtt ttatttagta
    13621 agaagttctt agttaaattc tcaataaaaa cggtatttcc atcttggtat atagtatatg
    13681 ttgggatagt tgcagctagt gttacggcac cagcatttaa tcaacttttg attggcaaaa
    13741 ttgctttttg gataggattt gtatcttata tagttcttat accagtaatt ttaaagagag
    13801 tatggataat caaggaaatg cctgaaccat ctttaccaag tatagctata ttagctgcac
    13861 cagggtcact attattgtct ggatatatta attctttttc agataaaaat aatgtagtat
    13921 tatacttgtt attcatatta tcaattgtat tttatattat agttttggga tacttaccga
    13981 aattattaag attgcctttt tatccgagtt ttgctgcatt tacattccct atggcaataa
    14041 gtgcaatggg tatgaaacta atgaatggtt atttaactaa atcaggtcat tttataagtt
    14101 ggatttcata tgcttctaag ttagaagaga taattgctac agtgattatt ttatatgttt
    14161 tagttttatt tattatatat cttagtaaat ctgaaactac atctaaagct aaataagaat
    14221 tattaaatat gtgtatttaa taggaatatt gatataaata tatgaagata agataaacca
    14281 tagaaactta atttagctta attttattgg aggaaaacaa atgaaaacaa aagaagaata
    14341 tttaaaagaa gttagaaaca tatcagaaga atactttaga aaaggagagt ttttttgctc
    14401 tgaagcagtt cttcagacaa taaatgatgc tttaggccaa cctttaagtc cagagataac
    14461 taaattagca tcaggatttc cgataggact tggaaaagct caatgccttt gtggagcgat
    14521 atctggagga gagatggctt taggtatagt atatggaaga gtacatggag aaactatgaa
    14581 tccaaagatg tttgagcatg ctaaaggttt gcatgattat attaaaaaag agtatggtgc
    14641 aacatgttgt agagctatta caaagaaatg ggatggtgat aactttatgt ctcccgaaag
    14701 aaaagagcac tgtattaaaa taacaggtca agttacagag tgggttgcag ataagcttat
    14761 agaagatgaa aaacttaata tcaaatagtg taatattagt tggtgaattt ataaagttaa
    14821 atttacttat agtataagat acttgatatt ctcgtattga tagaaaaatt tatagcatat
    14881 gtatttaatg aaatatactt atatttagag tgtggtttaa tttcataaat acatatgcta
    14941 ttattttaag tatataagca aatttattta aataaataga aacagttaat ttatatgtac
    15001 aaaactatat atgattagtg taaataaata cctattttat aatactcaac ataaagacac
    15061 cagacatgat tagtattata ccaataaaaa tagcaagcca ttcttttttg ttcttctttt
    15121 ctttaaaaaa tataatccct cctaaagtgg ctactatcac acaactctga gagatagaaa
    15181 aacttattgt aactcccaat gtattagtcg ttatgaacat acctaaattt gctattgacc
    15241 acaatatacc agtaattaaa ttgaataaaa cattttccct atatacaatc tctttagaaa
    15301 aatacttgac aataatagat gaagttatca acataccaac agattgaggt aatataattc
    15361 tatatccttc aattaaaaat atttgattag ttacaacata taatgataaa aatagagatg
    15421 ataataatac aactccaaca cattttaaca tatttttatc tgtagatttt ttagtttgat
    15481 aattagtgag aagtatacca agtattatac ataatattga taaagaacca atcagtaaca
    15541 ttttagaact ggtccattca ttaaacaaga ttacagctaa taatgtcgta aaagttaatt
    15601 gtgaaccatt tgaaattggc attactttag atacactagc aatttgaatt gctttaaact
    15661 gcattaattg tcccaaactc cagaatatgc cagaaatcat acttatacaa aatgaaaaaa
    15721 atgtaatttc aggatgtata accagagtta ttatagtgga aaatataaac gcacttatgg
    15781 atgtacctaa aagctgttca tgaggagaac ttttctttag atttgcaact atgggcatca
    15841 atccccatcc cattacagga agtaaagcaa taattattgt cagcattaaa ctatttcctt
    15901 tcatattaaa tttagacagc ttttaattta tcatctaaaa ttaaagtgtt caatacataa
    15961 aaattaccgt atttaatcag gaaatattag tagtttttta gaaaaacatt ttccattaaa
    16021 ataaagataa agggaaggag taaaattgaa aatgttaact gatatgattc aaaatcgtga
    16081 aattaaaata ataaagtttt tattaaaaga aggtcataca actgtaaaag caatagcaga
    16141 tgatttagat gtttcagaaa aaacagtttc aaaatctctt aaagaagtag aagcagtgct
    16201 taaaaagata ggtctttctt taataagaaa acctaagata ggtgtttata ttgatggaga
    16261 tttaaaaaaa atacttcctt acctagataa taaaggaagt caaattccta caaccagaga
    16321 ggagagagtt atctatatat attcagtact tttaaagagt aatgattata ttactataca
    16381 acaactttct gatgaactat atataagtcg tggaactatt gaaagggatt tatcagaagt
    16441 tgaaaaactg ctaaaaaacg aagggatttc catatataag aaacctagta agggaataaa
    16501 gttaaatata agtgaaagag ataaaagaca acttactgct aagtttatac ataaattttg
    16561 gagtagaaac tggtatatta agcaagagca agaaagcttt tatcaagcat ttgaaaagat
    16621 acaggcagat gtaaatggta ttttttcgga agatgattta tcaataatta ttgatattct
    16681 taggaatttc agcagagaaa gaaaatttga gttctctgat tatgaatttc agtcattagt
    16741 tattcatttg gcaatagctg tagagagaat tagaaaaggc gaatatatag aagaaagttt
    16801 gtatattggt aatatagaaa ataatcaaat gtcaaatacc aattatttag caaaacaatt
    16861 agaagaaaaa ttttcaatat tattaccaaa atctgaaatt ggatatataa atttacattt
    16921 agtagcggct gaccaacata gagatactaa tgaactaaaa aatacttcaa ctaatccttg
    16981 tgaaacaagt acaaataata tatcaaatct tcgggaaatt ataaaaaata cattgtatga
    17041 gacagcttat gacaaggatt taattgatgg attgacttca catgtacatt catcaataaa
    17101 tagactgaaa tatggactta gtattaaaaa cccatatgta aatacaatca aacaaaactt
    17161 ttctttatca tttgagcaag ctttagattt aaagaaagta attgaattta tatatgatat
    17221 tagaataaat gatgatgaat gtgcttatat agcattacat tttgaagctt ttagagaaag
    17281 aatgaaagaa cttccacaaa atattagagt aattctagta tgcagtacag gattaggttc
    17341 atctaggtta ttgtctgcaa gaataaaaaa atactttcca atgataaaga ttgaaaaagt
    17401 actttccgta caagaattag tatccagtaa aattgatgca gatttagtaa taagtactat
    17461 ttatttagag ttagaaacaa ttccaaccat agtagttagc cctattttaa atgaaagtga
    17521 tataagaacc ttggaaagaa atatacaaaa tgtttcatgt tgtacttcta agaagtataa
    17581 atctttcaga aacctaatct ttgaagaaaa tatattgtta aatattgaag cttcaaacta
    17641 tgaagatgca attaagtata taacagataa cctaataaaa aaaggttttg taaaagaagg
    17701 tatagctaaa agtgcaattg agagagaaaa tttatcttac acgtcttttc agtcaatagc
    17761 tacacctcat gctaatccta gttatattaa aaaatctaat atatctgttg caacgttaaa
    17821 aaatcctatt acatggggaa atgaaaaggt ggaagttatt ttctttattt cattagaaaa
    17881 tgacaaaaac ctagagcttg atgaaatata tgaagttttt tatgacttta ttgacaataa
    17941 aaaaaatata aatgacatat taagtgcaac ttccacaaag gaagtgtacg atttgctatt
    18001 ggaggagagt atatagtgaa aatatcagat gtattgaaaa aagagcaaat tgtttttaat
    18061 cttgatacaa atacaaagga agaaacaata aaaaaattaa gtagtatatt tgttgaaaat
    18121 gggattgtgg acaatgaaga agaatatgtt aagtctgttt tggaaagaga agaacattca
    18181 actacaggta ttggtaatgg tattgctatt cctcatggaa aaagtgatgc tgtcaaaaaa
    18241 tcttctataa tatttgcaaa gactaagcat aagattgagt gggatgcttt agatgaaaaa
    18301 ccagtagata gtattttctt attggctata agcaatattg ataaggatag caatcatctt
    18361 gtactattat ctaaattagc aactaaactt atggatgatg acaatgtaga tgcactaaaa
    18421 ttagccaatt cagaacaaga aataatcaat atatttagtg aagaaggaga gatgtagaaa
    18481 tgaaaagaaa aattatagca gttacagcat gtgcaacagg agttgcacat acatatatgg
    18541 cagctcaagc gttaaagaaa gcttcaaaaa agatgggtca ccttataaaa gttgaaacac
    18601 aaggagctac aggaatcgat aatgagttaa ctacaaagga tgcacaaatt gcagaagtag
    18661 taatatttgc agtagatact aaagtcagaa atgctgaacg ttttgaggga aaagaaatac
    18721 tagaagtacc agttaatgca cctataaagg atgcagagaa agtaataaat gatgcattaa
    18781 agttaattga cactaaataa atggagggaa atgtaatgtt gaaaaagata gtctctgagt
    18841 taaaaaagca tgtcttaact ggaatctcat atatgattcc attagtaatt gcaggtgcta
    18901 tgataatggc aatatctcgt gtaggtggtt ctttctataa tataccagat atatgggatg
    18961 ctaaatacgc agaaagtgct agtagtattg ttagattatt acatgatcta gatggatttg
    19021 gaggtactgc attaggatta atgtttcctg taattgctgc atttatagga ttttctatag
    19081 ctgataaatt agctattgta ccaggtcttg ttggaggtat gattgctaaa gatataggtg
    19141 caggattttt aggagctttg gcagttggtc ttatagcagg atatacatgt ttgtttatta
    19201 aaaatcatgt taaattacca aaatcagcag cttcaatagt accaatattt atagtaccag
    19261 tatttggaac attaatcact gttgtattaa tcaattatgt gataggtata ccttttgcca
    19321 cattaaatac tggattagaa aattggttaa atggattatc tggtacaaat cagatattaa
    19381 tggcagctat tataggggct atgatggcag ttgacttagg tggaccagtt aataaagcag
    19441 ctttaacaac atcacttgca ttattaacaa gtggaattta tgcacctaat actgctgcaa
    19501 tggtaggaat agttatacca ccactaggat tgggacttgc tactatacta gcaaaacaaa
    19561 aatataataa acagttacgt gaagctggga aatcatctct tataatgggt ttaattggag
    19621 taagtgaagg agcaattcct tttgcggtag aatctccatt aaaagtaata ccttcatgtg
    19681 taattggaac agcaattgct tcagctatgg cagttggttt aggttcagta aatgcaactc
    19741 caattagtgg attttatgga tggtttacag tagagaattg gccaatgtat gtgttatcaa
    19801 tagcagttgg aacagtaatt gtagctggat tagttatagt attgcgtgga aatccagtga
    19861 ttgaggatga tgaatttgaa gatgatgatt ttgatgaagc agagtgggaa tcttaaaatt
    19921 taataactat ttaagacgtg ttataagcta tattcaatat gaatcttata ctaaataaag
    19981 tgttaaatat tcagtttaga cttaaatata aaagataaag aacagaggag aataaatgat
    20041 aaaagcacat attgttaatc atacacactg ggatagagaa tggtatttta cttctggaga
    20101 tgcattagta cttagtgaac aactatttac agatgtaatt gatgaattag aacgtaatcc
    20161 agatgtaagt tttgtacttg atggacaact ttctattttg gatgattatg tacaattaca
    20221 caaagaaaaa atagaaatca ttaaaaagtt aataaaagaa gatagactac atattggacc
    20281 atggtttact caaactgatg cattctttgc tagaggagag tctattttaa gaaacttaat
    20341 gattggaatt tttgaaagta aaaagtatgg taagtatatg aaaattgggt atttgccaga
    20401 tacatttgga tttaatgcac agatgcctat actattaaag catgtaggtc ttgataatat
    20461 attcttttgg agaggtatac atcttggtaa acatgttcaa tcaccatatt ttaagtggaa
    20521 aagcttaaat ggagaaagtt atatatatgc agtaaatatg ccacatggat atggaactgg
    20581 tatgctttta gagccttcat taaagtatgt taatggacgc ttagacccag ctattgattt
    20641 tattaaatca tatactgatg tagatgaggt gttaatacct tctgggaatg accagttaaa
    20701 tataataggt gatttcaaaa ataaaattaa agcgattaat gatatcggaa agtattcata
    20761 tataacgagt tcatatcaag agtttttaaa atatataaag tccatagaat cactagaaag
    20821 ctatagaggt gaatttagag aacctgtatt agcaagaata cataaatcaa ttggttctag
    20881 tagaatggat ataaaattag catgtgataa actagaaaac aagctgatta aaagaataga
    20941 gccattatta gtaattgcta aaaagagtaa aattgaaata agcaatcagt tacttataaa
    21001 tacatggaaa aaacttttgg aaggacaagc acacgatagt ctagctggat gtgtaactga
    21061 tactgtaaca gattatattc ttcaccgtat aagagaagca aatgaaatat gtgacagtat
    21121 tgaaaatact attgtaaaaa agatttcaga agggcttaaa ttatctaaaa atgatatttt
    21181 agtatttaat acagaagcta agcgttttaa tggatataaa gagatacaag ttgtaagtga
    21241 tagtaaaaat atttatttta aagataattt agatgctact ataattgaag aaacttatgt
    21301 aaaaccgaga gaaaatgtat tagaagaaac acctgctggc aacgtattta ttgaagaatc
    21361 aggatattat atattaaaag taagaataaa tgtacagctt cctgcacttg gttataaagt
    21421 tgttagtttt gaatcaagtg ataaagaaat ggtatcactt gataaatcta atgacacttt
    21481 tatatctaat gataattata agataatcta tgaagatgga agcttaaatt tacaacttaa
    21541 agatggaact tacatatcaa actttttaat attaaaagat agtggtaatg caggcgatac
    21601 ctatgacttt tctccattaa aaaatgacga ggatataaaa ctttcatttg atagagtgta
    21661 tacagaaaaa actttaggat atgaaaagtt ggtggttaaa ggtactactt tactaccact
    21721 tacattagaa gatagaaaaa acaaatatct taatgggaaa ttaaatgtaa aagtaagtat
    21781 tgtcttatcc aaagaaaatc cattgatgga tgtaaaactt tgtgttgata acacgatata
    21841 taatcataga ctacgtgttc atataaaaac tgatataaaa gataataaaa acatagcatc
    21901 attgccattt ggatatataa caagagaaaa tggagtttta gacaactggg aagatattta
    21961 cagtgaaatg cctatagatt tagaaccttt ggaaagtaat attactctaa caaaccaaaa
    22021 tagaagctgt actgtattta ctagaggaat aaaagagtat caacatattg aagatgaaat
    22081 tgcattgacc ttacttgcta ctacagatga actaggaaag cctgatttat tatatagacc
    22141 aggacgtgct tctggtgata caactaaaaa aggtcatata agaattaaaa cagataaagc
    22201 tcaaatgctt aagaaattag aattttcatt tgcaatatat atggattcta aatcatttga
    22261 tgaattggaa attgcgaatt taacccacaa ttatctaagg gaatgtgtaa attatcaatt
    22321 acaggattac aatttctttt tatatcgtat agataataag attcaaaaat caattgtcaa
    22381 taaaactgta agtagagaat tagaaataat atctttacca gaaaaatatc ttatatcagc
    22441 atgttatcct tcttattaca gtaaagataa atttataata agagtggaaa atccaagtag
    22501 taatagaatg atattggatg agcaaatatt taaaaataga aatgggaaaa ttgtaaatgc
    22561 aatagaagat gtagaagaag aacaggtata tgaaatagca ccttttgacg ttatttcaat
    22621 attattggat ttataagtgt aaaagtaagt ttattgttta aagtctgaca gatattaatc
    22681 tgccagactt tttaatttgt ataaatttgg gtaaatatta atgaatagtt ttagtattta
    22741 aatatgaatt tagtatttaa tatagaattt attgaagt
//
LOCUS       DAEPLF010000040        22664 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEPLF010000040 DAEPLF010000000
VERSION     DAEPLF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.040"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            140..1180
                     /locus_tag="KR314_001387"
     CDS             140..1180
                     /locus_tag="KR314_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0327778.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1536..2858
                     /locus_tag="KR314_001388"
     CDS             1536..2858
                     /locus_tag="KR314_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBH0327779.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3156..4517
                     /locus_tag="KR314_001389"
     CDS             3156..4517
                     /locus_tag="KR314_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBH0327780.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4590..5984
                     /locus_tag="KR314_001390"
     CDS             4590..5984
                     /locus_tag="KR314_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0327781.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQLAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6046..6516
                     /locus_tag="KR314_001391"
     CDS             6046..6516
                     /locus_tag="KR314_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBH0327782.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIKEAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6519..8735
                     /locus_tag="KR314_001392"
     CDS             6519..8735
                     /locus_tag="KR314_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0327783.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHNIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFRGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLIVEEAEKLLETSKEDLYLDNGYVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGLTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVNSYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8818..9489
                     /locus_tag="KR314_001393"
     CDS             8818..9489
                     /locus_tag="KR314_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0327784.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9852..11066
                     /gene="dpaL"
                     /locus_tag="KR314_001394"
     CDS             9852..11066
                     /gene="dpaL"
                     /locus_tag="KR314_001394"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBH0327785.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            11193..12380
                     /locus_tag="KR314_001395"
     CDS             11193..12380
                     /locus_tag="KR314_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBH0327786.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            12513..13883
                     /gene="hydA"
                     /locus_tag="KR314_001396"
     CDS             12513..13883
                     /gene="hydA"
                     /locus_tag="KR314_001396"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBH0327787.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKN
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            13901..15307
                     /locus_tag="KR314_001397"
     CDS             13901..15307
                     /locus_tag="KR314_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0327788.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            15379..15825
                     /locus_tag="KR314_001398"
     CDS             15379..15825
                     /locus_tag="KR314_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBH0327789.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            15864..16658
                     /locus_tag="KR314_001399"
     CDS             15864..16658
                     /locus_tag="KR314_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBH0327790.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            16648..18939
                     /locus_tag="KR314_001400"
     CDS             16648..18939
                     /locus_tag="KR314_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0327791.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDTKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            18940..20253
                     /locus_tag="KR314_001401"
     CDS             18940..20253
                     /locus_tag="KR314_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0327792.1"
                     /translation="MIAIKSKYLISSADEIYTNSAAVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            20289..21374
                     /locus_tag="KR314_001402"
     CDS             20289..21374
                     /locus_tag="KR314_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBH0327793.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            21604..>22664
                     /locus_tag="KR314_001403"
     CDS             21604..>22664
                     /locus_tag="KR314_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327794.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEMTVMKEDISEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVKQEINVLKEDMSEVKQ
                     EMIVMKEDMSEVKQEMTVMKEDMSEVKQEMTVMKEDMSEVRQEINIMKEDISEVRQEM
                     TVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINV
                     MKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIE
                     IKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKED
                     IAEIRRDLNLVELATSKNW"
BASE COUNT         8819 a         2500 c         4620 g         6725 t
ORIGIN      
        1 actaagtaga tatacttttt attaagtttt gttttaaaat aatattatta aattataact
       61 aagcttttct gttatgatat aattaaatag ataatgtgaa tgattattat atagaatact
      121 tatttggagt tgatatttta tggacaaaca cagtgattca gtaatgtatg gtggaatgga
      181 agtatataat atttggggaa caacaacagt agactccata attggagagt ctgatgtaat
      241 gcgtgctctt aagaatagaa tttgcaaaat tggaaatagt gattctactg tagctataac
      301 tggagaaagt ggcactggaa aagaattaat agcaagagcc attcatctcg caggaaacag
      361 agggaataaa acattcgttg ctataaactg tgctgctata ccagaacctt tactagaaag
      421 tgaacttttt ggatatacaa aaggagcttt tagtggagca gattcaaaag gtaaaattgg
      481 taagtttgaa ttggccaatg gaggggttgt atttttagat gagataggag atatgccaat
      541 tcaactacaa tcaaagttat tgagggtatt acaggaaaag aagtttacga gaataggttc
      601 aaatgaacta attgatatag atgttagaat catatcagct actaatagag atttatttga
      661 gttggttaag gaaaataaat ttagagaaga cttatactat agattgaatg taataccttt
      721 agaagtgcct ccacttagag aaagaggaga cgatatacaa atattaataa ataattttat
      781 agacaaatat tcgagaaaaa tgaagaaaaa cgtgtaccat atagaaccag atgtggaaca
      841 aatgttgctt aagtatcctt ggccaggaaa tattagagag ttggaaaata ctatagaact
      901 ggcattaaac cttcttgagg aagatggaat aatacataaa ggaataataa atagaaagat
      961 acttgaatat tttaagttaa ataatataaa tataaagtta cttgaggaaa gtgactatga
     1021 gataagtatg gaacaagaca tacttacact tgaagagttt gaaagagcct atataaagaa
     1081 agtgttaaag ttttacggaa atgatactaa gtctaaaaaa ctggctgcca aaaaacttgg
     1141 aatatcacta gctacattgt acagaaaaat agatgtataa taattaggtt ttttattctc
     1201 aattctaaaa tttgaaatta taaaattgat aaaaaacact tggaaaacta ttttctaagt
     1261 gtttttttgt gtaatagcat tttattatcc aaaaataaaa aaaatttaaa agaatttaaa
     1321 aaaataaatg ctttaataaa ttatcaaata ctaacaaata taataaaaat tatatatgta
     1381 aaacaattat gttaaaaaat tggcattata attgcttcat attaaattga gatgacaaaa
     1441 taaaaaaaga aaggatgttg tgtgagataa taataactgt cattaaaaaa tagttgggag
     1501 tgataacctt gagtaaatat caaaagaatg tttctatgtt tgatattgat ggaaggccac
     1561 caattttaaa gtgtttgcca ctatcaatgc aacatatact agcaatgata gttggaactg
     1621 ttacagtacc tatagtcgta ggaggagctg tgggtgttag ccctacacaa ataacacttt
     1681 tagtgcaata tgcattaata atagctggta tatcaacact tatacaacta tatccaatag
     1741 gaaatattgg ttcaagattg cctataattt ttggagttgg ttttacttat gtaccaacac
     1801 ttgtagcagt cggaggaaat tatggattag caagcatttt gggtgcacaa ttaatagggg
     1861 gagtggctac agtattaata gggatattca taaaaaagat aagaaaattt tttccgaata
     1921 tagttgcagg aacagtggtt tttacaattg gattatcttt gtatcctata gcaataaatt
     1981 atatggcagg tggtgtaaat agtcctactt atgcctctat gcaaaactgg gcagtagcgg
     2041 ctataacatt agtggtagtt ataggtctta gtcaatttgc aaaaggatat gcaaaattgg
     2101 cagcaatatt tttgggaatt atagttggat attgtgtatc tttagttctg ggtataataa
     2161 attttgaccc tattagagag gcaacttggt ttgcgatacc taaaccgctt gaatttggga
     2221 tggaatttga tatgcctgta ataatctcag tagtaattgt aagtataatc aatgctgttc
     2281 aagcaattgg agatttatca gctactacaa tgggaggcat ggataggaat atcacagata
     2341 aggaattatc aagtggagta ataggaagtg gaatttgtac tatgatagga gctttgtttg
     2401 gaggtttacc accatcatca tatagccaaa acgtggggat ggtagctatg aacaaagtaa
     2461 taagtagatt tgtgcttgga atagctggta tatttatgtt attgtcgggg tttgtgccta
     2521 aatttggtgc attaatgact actataccat attctgtttt aggaggagct acaatatcta
     2581 tatttagtat gataacaatg acaggtataa agttaattat tacagaagag ttgtcatcaa
     2641 gaaatattac cattgttggt ttggcaattg ccttaggcat gggaataaca tcagtaccag
     2701 cagcccaaga ggcatttcct tcatttgtaa agttagtatt tggagaatct ccaatagtaa
     2761 ttgcaacatt ggttgcattc gttttaaatc taataatacc aaataaaagt ctagaggatg
     2821 aggctaaaga aagagctgtt atggaagaga ctaattaaga tataataatt taaaagaatt
     2881 gtttaaatta tttagtatga gattgttaga ttatttttga tagctattca ttttaataaa
     2941 aaatttaaca aataaatata taccaggcaa ttaatgtttc atttaagttg aaaatcgggg
     3001 agggaatatt tagaaatttg ttaatttttt ataaaaatag ctcagtttag ctgtgtttat
     3061 cggttgagca ataaattcat ggtcataaaa atatttatag aaaataatta taaaaatatt
     3121 tagtcaagat atttatgtat gggaggattt attttatgaa aacacgtaat acttcaaaat
     3181 atgatgtcga tgggatacca ccactaaagg aatcaatacc tttggcctta caacatctgt
     3241 tagcaatgat agtaggaaat atggtacctg cgatattaat agcaaacgtg gtaggactta
     3301 accaggggca agcaacaatg ttgattcagg gttctatgtt agcagcaggt ttagcaacgt
     3361 ttttacagtt atatccaata cctttattta aaggatttaa gttaggttca agattacctg
     3421 taatgatggg aatgagttat gtatttttag gagcttgtct ttcagtagca gctaaagatg
     3481 gtttagcagc tctatttgga gcacagattg ctgctggggt aatcgtattc tttgtaggat
     3541 ttggagttaa gaaaattaga catatattca ctccaatagt ttctggaact ataatagcat
     3601 gtatgggact tggactattc gcaacagcta taaagaattt agctggagga gaaggcacac
     3661 aaacatttgg aagcccaata aacttcacag taggagttat agttgctttt gtaataataa
     3721 tgataaataa atacggtaaa ggtttagtaa aaaattcttc aatattaata ggaatattag
     3781 taggttatgc tatatcttta gttttaggat tggttgattt ctcagccgtt caaggagcag
     3841 ctatagtatc tttaccaaca ccagcagctt ttggattaga atttagacca gagttaatag
     3901 taatgtttac tattatttac ataataggta tagctgatat gatgggtgcc tgtacaatag
     3961 ctacagtagg agcaatggat agagaggtaa ctgacgaaga attagcatca gttgttttag
     4021 gaaattctat aacttctata atttcatcac tatttgcagc tcttccaact ggagtattta
     4081 gccaaaatac agtaattgta tctatgaaca aagttacaag tagatttgta atagcactag
     4141 gggcacttgt attattacta gcagggatat ctccagcatt gggtgcaata atgacaacta
     4201 taccatcttg tgttgttggt ggagcaactt tagtagtttt ctcatcaata gcaatgtctg
     4261 gtttctctat tatgagtatg gatggattca ctgaagaaaa caatcttata gcaggtgtat
     4321 ctatagcaac aagtatgggt cttacaactg ctccacaagt tttagaccaa ttcccagaaa
     4381 caattggaac tgtccttgga gggtcaagta tagtttctgg agcaataata gctatattac
     4441 ttcaaacatt atttaaatta aagtcacgta aaagtgctga aaacgttact tcaaatcttg
     4501 aggaaaatat tggataaatg tattagatat aatatgagaa aaccatatgg taaaatgttg
     4561 taatgaaagt attcaaaggg ggatttaaga tgattgatat actaataaaa aatgcaataa
     4621 ttgttacagt taataaagac agagatgtta tatttgatgg agcaatagct attcaaggtg
     4681 acagaatctt ggatatagga aatacagttg atattgagcc taagtatctt gatgctaaaa
     4741 aagttataga tgctaatgga aaagtaatat tccctggctt tataaatact cataatcacc
     4801 tgttccaagt gttattaaaa ggtttaggtg atgatatggc acttcatgag tggttaaata
     4861 caatgatgtt tccatcagca aaattcttaa cagaacaaga tacttatgat gctgcaatgc
     4921 ttggatgtat ggaaggttta aaaagtggta taactacaat ggttgattat atgcatactc
     4981 acaatagacc aggacttaca gatggaatag taaaggcata taaagattta ggtataagag
     5041 gggttgtagg tagaggttgt atagatttag gtattcacaa agagcttata gaagatgtgg
     5101 aaacggttga aaaagatttg agaagggtat tcgaaaaata tcataattca gaaaatggaa
     5161 gaataaaaat ctgtgtagct ccttcttcaa tgtgggctat atctgaagaa atgggtaaga
     5221 tgctttggaa tatagttaaa gaatatgatt catattttac agttcatata tctgaaacag
     5281 aatttgcaag aacagcaaca aaggatatac atggagaaat agatataaaa ttacttgaaa
     5341 aatggggtat agtaggtcca gaagttgtag cagtacattg tgtatgtata actgatgaag
     5401 acatggaaat gcttaaaaag tatgacataa aagtttctca taatgtagca agtaatatgt
     5461 atcttgcttc tggtgttgca cctgtgccag aaatgttaaa aaaaggtata aatgtgagtt
     5521 taggtcttga cggtgcagca agtaataacg cacaagatat ggtagagctt atgaaattaa
     5581 cagctttaca acacaaagta aataattgtg accctctggc aatttctgca gagaaagttc
     5641 ttgaaatggc tacaattgag ggagcaagaa ctcttagaat ggaagatgaa ataggttctt
     5701 tggagattgg gaaaaaagct gacctagtaa tatttaactc aatgctttct ccaaaatcaa
     5761 tacctcttca caatcctgta tcaactttgg tatactcagc tagtatgcac aatgtagaga
     5821 gtgttttagt tgatggaaat gtaatacttg aaaatggaaa agtaactaca attgaaagtg
     5881 aagaacaagt gttgcttaat gcacaacttg cagcagaaga attatgtaag agagctaatg
     5941 taactaatag aatggaagga cataagtgga atagcttata ctaatattaa aataagattg
     6001 taataatatt ctattacaac attagtttat ctgtaggagg agaaaatgga aaaaataaaa
     6061 ttaaatatta atgattacat gtatgaaata gaagttgaaa agaatgaaac tcttttgcat
     6121 gtacttcgtg aaaaattaaa tctaactgga gcgaaacaag gttgtaatag tggcagttgt
     6181 ggagcatgta aagttttgat agatggagtt gacatgaaat cttgtaaact tcttgcaaaa
     6241 aactgcgaag gtaaagttat atacactata gaaagttttg ctaatggaga aaatcttcat
     6301 ccaatacaag aagcttttat agaagttgga gctgtacaat gtggatattg tactcctggt
     6361 atgattataa caacacaagc tcttttaaat agaaatgcat ctcctaatga agatgaaatt
     6421 aaagaagctt ttaaagaaaa tctctgtaga tgcacaggat atgtaaagat tatagaagct
     6481 gtaaaattat ctgcagctag attagggggt agataattat ggaaaaggta ataggaaaat
     6541 cataccctct aaaagatgca gctttaaaag ttactggtca aatgaaatac gtggctgata
     6601 tgaaaccaca aaatgtactt tacgctaaga tgcttttaag tcctgtagca catgcaaaaa
     6661 taaaaagtat agatacaagt gaagctgaga aagtagtagg tgttagagct atagctactt
     6721 atctcaatac atcacaaaga aaatacaatg gttcattaag attttatgag cacaatatac
     6781 ctgaaaatga agttatattt tcagatacag ttagatttgt tggtgaccgt gttgcagcag
     6841 tagctgcaga gactccagaa atagcagaga aagctgtaaa acttataaaa gttgaatatg
     6901 aagaattgcc agctgtattt actatacaag atgcaataaa agaagatgct atctgcatac
     6961 atggagattc aaatatagtt tctcaaaaca acataaatgg gggagatgta gaaaaaggct
     7021 tcaaggaatg tgactatata tttgaagatg aatactctac tccagcaata catcatagtg
     7081 caatagagac tcatagtgta attgcacact atgattataa tgataaatta actgtacata
     7141 caccttgcca aaatacattt ggatttagaa taatactatc tcaaattttt gggatgtcat
     7201 taaataaagt aagagttata agacctgcta ttggaggctc atttggtgga aaattagaac
     7261 ttacaattga accagtagca gccgcattat caaaaatgac aagaagacca gttaaaatgg
     7321 ttcttacaag aaaggaatct atgatatcaa caagaactcg tcatgggtct tttgtaaaat
     7381 taaaaactgg tgtaaataaa gatggtgaag taatagcaca agatataaaa atatatacaa
     7441 atacaggagc atatgcatca agtgctttaa atgttatagg agcacttagt cataaagtat
     7501 ttaaagttta taaaatacca aatataaagt ttactgggat gcctgtatat acaaatacac
     7561 caatagcagg agctatgaga ggctatgggt cacctcaaat atttatggct caacaggctc
     7621 aatttgcgaa aatagccaag gaaataggta tggatttagt cgattttcaa aataaaaatg
     7681 cagtagagcc agatgatgtc gatataatct tccgcggttc tcttggaaat cctagagtat
     7741 tggattgtat agagcaaggt aagaagatgt ttaagtggga tgaaaagaaa aaacttccaa
     7801 aagaagaagg aaaatattta agaggtatag gtatggcaat aggtgcacat ggtaatggag
     7861 tttttggtgc acatagagat gttgttacat tgactcttaa attaaatgaa gatggtacat
     7921 tgacactact aacaggagca catgatatgg gtaatggtgt cgtaactatg caaacaatga
     7981 tggtagctga agttttagga ataacacctg ataaagttga tacatttgaa actgatacgg
     8041 atgcatgtac attcaacctt ggtgactatg caagtagagg ggtatttgta gaaggtggag
     8101 gagctaaaaa gacagcagag aaattgaaaa accttatagt agaagaagca gaaaaattac
     8161 ttgaaacatc aaaagaagat ttatatctag acaatggata tgtaattagt aaacttgatg
     8221 aaaatgtaaa agcatcactt tctgatgtcg cagtatatag tcaaagtaaa tcattaagag
     8281 agttaactgt tgttgaagat tattcttcac cagctggact tacttcttat ggtgtacatt
     8341 ttgctgaagt tttagtagat aaagaaacta aggatataaa agtagttgat tttgtagctg
     8401 tacatgatgt tggtagagtt ataaatcctt taaatttaga aggtcaatta gaaggtggga
     8461 ttcatatggg tctaggatac gcattgagtg aagaattggc atttgatgaa caaggtagac
     8521 ctaaagctac tgattttaaa acatataaat tatttagaac tgtagatatg ccaaaatgtc
     8581 aagttgcatt tgtaaatagt tatgaaaaag ctggaccttt tggtggtaag agtattggag
     8641 aatgtgctgt tgtaccagtt gcgccagctg ttgcaaatgc agtatataat gcaactgatg
     8701 tagaaataca ttcattgcca ataaaattaa aatagtagaa attaaagcac aaacaacaat
     8761 ttattgtatt tgtaaataga taataaaaat agatttattt ggtgaggaga tattattatg
     8821 gcaaagatat taggtatttg tggaagtcca cgtaaaggag caacagaata tgcagtgtta
     8881 gaagctttga aagaagcaga aactatacct ggtatagaaa ctgagtattg gtcagttaga
     8941 gggaaaaaaa taagtccttg tgtacattgt gatgcatgta taagaaaaga aactatgtgt
     9001 attataaaag atgatatcca agaattagag caaaaaatat tggaggcaga tggaattata
     9061 gtaggttctc cagtttatga tatgaatata actgctcaac ttacagcagt atttaataga
     9121 cttcgtccaa tgtatttagt tcatccagga aaacttcaaa ataaggttgg tgcagctata
     9181 actactggtg gaactagata tggtggccaa gaattgacaa aacttcctat aataaatttc
     9241 ttcctaatgc atgaaatgtt agtatctgga ggtttaggtg gatgctatat aggtggtaca
     9301 atatggagta aagatggaaa agcaaaaggt gcccaagagg atgagacagg tatggacaca
     9361 gtaaaaagac ttggaaaagg tgctgcagag gcagttatgg tttctaagtt tggtcttgaa
     9421 aaatggaatg tcgtaaaaga agaattagat gttaaaaaag atgaaaaatc acctcttaga
     9481 gaccactaaa gatttattat attattaaat atatacaaat agtgatgtaa tagattattg
     9541 catcactatt ttatgtttta atttagattt tataaatttt atattaaaaa atatagaaac
     9601 aaagtgtttt gagatttatt tttaaatttt aaaaatgaga attaaaaagt gagaagatag
     9661 aaaaggtttt ttagttatta aattctacat acattgtatg aactaaagat agaaaagaaa
     9721 gaataataaa agcattgcaa tttctatact aaagagtcat agaaaaatat ctagtatata
     9781 tttagtgttg gcatgttaat tgcttatata ttaaagtata aaaatttaac aataatttgg
     9841 aggtatttta gatgttaaaa gaaattaaat ggaaagtaaa taatttgcca aagggtgata
     9901 aggaaaattg tatcaaattt ttaaatgaag aagaaataac taaggttaga aattttcaca
     9961 aaagctttcc tcaatataaa gaaactccat tagcaaacct agaaggttta gcaaaaaaat
    10021 taggtgttgc aggagtatat gtaaaagatg aatcttatag atttggatta aatgcattta
    10081 aagtattagg tggttcttat tctatgggta gatacttagc tcaaagatta gatacagata
    10141 tatctgagtt agggtatgac aaattaactt cgaaagaaat aaaagaaaaa ttaggagaaa
    10201 taactttctt tacagctaca gatggaaatc acggtagagg agttgcttgg acagctaata
    10261 aattaggtca aaaatctgtt gtattaatgc caaaaggttc ttctgagttt agattaaata
    10321 aaattaaagg agaaggagca gatgctagta taacagactt aaactatgat gatgcagtaa
    10381 gattagctaa tgattatgca gaagctgatg atcatggagt aatggttcaa gatacagcat
    10441 gggatggata tgaagaaatt ccagcatgga taatgcaagg atatggaact atggctcaag
    10501 aagcgataga gcaattaaaa gaatatggag tagatagacc aacacatgta tttgtgcaag
    10561 caggggttgg ttcacttgct ggagcagttc aaggatatgt tgcttcaata tacgatgaat
    10621 gcccaataac agtagtagta gaagctgatg aagctgattg ctactataaa tcagcagaag
    10681 caggagatgg aaaaccaaga tttgttggag gagatatgcc aactataatg gctggtcttg
    10741 cttgtggaga accaaatact ataggatttg aagtattaaa aaatcatgct gcagcatttg
    10801 tttcagcacc agactgggtt tctgctaaag gtatgagaac attaggtaat cctttaaatg
    10861 gtgatgaaaa ggtaatttct ggagaatctg gagcagttac aactggtctt ttagtagcag
    10921 ctatggaaag agaagattta gcagatttaa gaaaagattt aaaattagat gaaaattcaa
    10981 gaatactttt aataagcaca gaaggagata ctgaccctga caaatataga agtatagttt
    11041 gggatggaga atatccaagt atataaggaa ttactaaaat gcaattatta gatattggtt
    11101 tacatattta gaagatatat aagttctaaa atttagaaaa tatataagta tataaaatac
    11161 atacattata aaaaatctag ggggataatg aaatgttaaa cgaacaaaga aaacaagaag
    11221 taactgaaat atgccaaaaa ttaattcaaa atcctagttc ttctggaaat gaagaaggtg
    11281 ttgtaaaagc aatagaagag tcttttgaaa aattaggttt tgatagttgg tcaagagata
    11341 gatatggaaa tatagtagga tgtataaaag gtaacaagcc aggtaagaaa atattatttg
    11401 atggacatat agatacagtt ccagttccag atgcaagtaa atggagtgtt cctccttttg
    11461 aaggaaaaat agttgatgga aagatatatg gtagaggaac ttctgacatg aaaggtcaat
    11521 atactgcaat gatgtctgct gtagcgtatt ttgctaaaga cactaatcga gattttgctg
    11581 gagaactata tgtagcaggt gttgttcatg aagaaatatt tgaaggtgtt tcggctagag
    11641 agataagtaa ggctgttcaa cctgactatg ttgtaatagg tgagtcttca gagttaaact
    11701 taaagattgg tcagagaggt agaggagaaa tagtagtaga aacttttggt aaaccagctc
    11761 actctgctaa tccagaaaaa ggtgtaaatg cagtttataa aatggctaat gtaatccaaa
    11821 agatacaaca attagtacct ccaactcatc cagttttagg tgatggaata ttagttttaa
    11881 cagatataaa atcttctcca tatccagggg catcagtagt gccagattat tgcaaagcta
    11941 cttttgatag aagattatta gttggagaaa caagagaagg cgtattagct ccaatacaag
    12001 ctttattaga tgaaatgatg aaagaagacc cagaattaaa tgcgaaagtt tcttatgcgg
    12061 ttgaaaaagc tgattgctat acaggaaata ctatagaaag tgaaagattc ttcccaggtt
    12121 ggttatatga cgaagaagat gaatttgttc aagcagcata taagggatta aaagaagctg
    12181 gaatagattc agaaataact caatactcat tctgtacgaa tggaagtcac tatgcaggtg
    12241 aagctggaat aaaaactata ggatttggtc cttctaagga aaatcttgct cacacaatag
    12301 atgagtatat agagcaagaa cagttattta taggaacaga aggttactat ggaatattaa
    12361 aatctgtata cggaaaataa tatttagttc acaataaatt atttaaatat agtctagagg
    12421 tgctgaatat agcaccttta gaaaatacaa ttttaaataa agttttacga tacttagaaa
    12481 atactttaaa gcttaaaagt taggaggtat atatgggaac aatactaaga ggtggaacta
    12541 taattacctc tgataagact tatataagtg atttaaggat agaagatgaa aagatagttg
    12601 aaataggaaa taacttagaa ataaatggtg ataaggttat tgatgctaca ggtaaaattg
    12661 taattcctgg tggaatagac acacatactc attttgatat gaatgcaggt tctattacaa
    12721 ctgcagataa ttttgaaaca ggaacaagag cagctatagc tggaggaact actacaatac
    12781 tagattttgc agaagccaat gagggggaaa acctactgca aggtgtagaa gcatatcata
    12841 aaaaagcaag tggaaattgt tattgtgatt atggttttca tatgacaatt acatgtttaa
    12901 ataaagatac atttaatcat atggaaaaac ttatttcgga tgggatagta tcttttaaga
    12961 tgtatatggc atatgatggc atgaaagtag atgatggaac aatatacagg gttttaaaga
    13021 aagcaaggga tttaggttgt atagttggat ttcattgtga aaatggggat ttactagatg
    13081 tattgataga agaaaatata caaaatggta atttagagcc aaaatatcat ccacttacaa
    13141 gacctaacat agttgagaaa gagtctgttt caagacttgc tgatataact aaattatcta
    13201 attcaaaatc ctatgtagtc cacttaagtt gtagggagtc acttgaaact gtaaaaatgg
    13261 ctagagaaaa aaatatagat atgatagtag aaacatgccc acagtatcta ttattagaag
    13321 acaatttata taataagaat aaatttgagg gtgctaagta tgtaatgtct cctcctttaa
    13381 gaaaaaaaga agacataaat tacctttggg aaggattgtc tagtggagat attcaaacag
    13441 ttggcacaga ccactgttca tttaatttta aggggcaaaa agacttggga attgatgatt
    13501 ttagtaagat accaaatgga gcacctggtg ttgaacatag gttagcactt ttatatacat
    13561 atggggtctt agaaaataga ataagtgtaa ataagtttgt ggaagttact tctacgaatg
    13621 ctgcaaaaat atttggaatg tatcctaaaa aaggtgagat agctgtagga agtgatgcag
    13681 atatagtcat tttaaatatc aataaagaag aaacaattag ttataaaact caaaaacaaa
    13741 atgtagatta tactccatat gaaggattta aggttaaatg taaagttgaa gatgtatttt
    13801 tgagaggaaa tcatgtagta caaagttgta atgttaaaga acatccaaca ggtcaatata
    13861 ttaaaagaaa aattactaaa taatgcgggg ggaaataaga atgatggata tacttattaa
    13921 gaatgccata atagtaactg tgaataaaga gagagaagtt atttttgatg gggcattagt
    13981 agtaaaagat aacaagattg ctgatattgg aaattctaaa gagatagaaa gtaaatatac
    14041 tgatgttaaa aaaattatag atgctaaagg taaagtttta ttcccaggtt ttataaatac
    14101 acataatcat ttattccaaa ctttattaaa aggattggga gatgatatgg tacttaaaga
    14161 ctggttagaa actatgacat ttccagcagc taactattta gagccaaaag atacttatga
    14221 tgctgcaatg cttggatgta ttgaaggact tagaagtggt ataacgacca tggttgacta
    14281 catgtatcct catagtaagc caggtctttg tgatggaata atagatgctt ataaagaatt
    14341 aggaataaga ggaatacttg gaagaggatg tatgaataca ggagctcaat ttggagttca
    14401 tcctggaata atgcaagatg tagaaacggt tgaaaaagat gtaagaagat tgtttgaaaa
    14461 acaccataat acagaaaatg gaagaatcaa aataggagta gctcctgcag caatatggtc
    14521 aaattctcaa gaaatgcttg aaatgctatg gagagtggta aaagaatatg atgatgcttt
    14581 atttacagta catatatcag agactccttt tgatagagaa gcagcaaaag aattgcatgg
    14641 gcaatatgac atagatgttt tagaaaaatt aggaatactt ggaccaaatg tgcttatggt
    14701 acattgtgtg tatctaactg aaaaagatat ggaacttact aaaaaatacg atatgaaagt
    14761 gtctcataat acagcaagta atatgtactt atcttcaggt gttgcaccag tgccagaaat
    14821 gcttaaaaaa ggaataacag ttagtttagg agtagatgga gccgctagta ataactctca
    14881 agatatgctt gaacttatga aattaacagc acttcaacat aaagttaata agtgtgaccc
    14941 attagcaatg tcggcagaaa aagttcttga gttggcaact atagatggag caagagctat
    15001 tggaatggaa gatgagatag gttcattgga gataggtaaa aaagcagact tactaatatt
    15061 taatccaatg ctttcaccaa aagcgatacc tatgcataat ccggtatcta cattagtata
    15121 ttcttctagt atgaaaaata tagagagtgt tatagtagat ggaaatataa taatggaaga
    15181 ttcaaaaata ttgacagcta atgaagagaa agcattaaaa gatgctcaag atacagctga
    15241 gagactttgt gtaagaggaa ctataaaaaa tagaatggaa ggtcataaat ggaatagcct
    15301 ttattaataa atgtaattta ttcatagaaa agtatagtaa aactttagct taaaaatatg
    15361 agttttgagg tgatttattt gattatcaaa tgtaccataa ataataaaga aaaagaatta
    15421 agtataagac cagatgattt tttagcagat acattaagag aaaatggata tgtaagtgtt
    15481 aaaaaagggt gtgatacagg ctcatgtggg ttatgtacag tgatggtaaa tgacaaggcc
    15541 gtgctttcat gtaattatct tactgctaga gtagatggtc agcatattac aacacttgag
    15601 ggggtcttgg atgaagcaaa gaagtttgga gattttcttg cagacgaagg ggctgaccag
    15661 tgtggatttt gtgctcctgg atttataatg atggttttgg caatgaaaag agagcttcaa
    15721 aatccaacag aagaagaagt tatagattat gtaaatggta acctttgtag atgtacagga
    15781 tataaaggtc aattaagagc aatacataaa tatttaaaca cttagacttt taagtgaaaa
    15841 ttaagttgat atggggagag agtatgataa ctataaagga atatgtagtt ccaaaaagtt
    15901 tagatgaagc ttatgaactg ctaatatcaa gaaaaaacaa cataatactt ggaggatgtg
    15961 gatttttaaa actaggtagt aaaaatattg gctcagcaat agatttaaaa gacttagctt
    16021 tagattatat aaatgaaact gatgatagca tattaattgg agcagacaca agtttaagaa
    16081 ctttagaatt aaataaggtt attaaaaatt actgtaatgg ggtaatttca aacgctgttt
    16141 ctaatatagt aggagttcaa tttagaagtg gagccagagt aggagctagc gtatttgcta
    16201 agtatggatt ttcagattta ataccttcat tactagtggt agatgcaaag gttaagttat
    16261 ataaaaaagg ggttatgaac ttagctgatt ttttagaaag tgagcttgaa aaagatatat
    16321 taatagaagt tatattacct aaaaaagatg ctataggtgt atttgattct ataagaaaat
    16381 gtacaggtga ttttgcagta ttaaatggag caatgttaaa agaaggaagt atttataaaa
    16441 ttgcgatagg cgctagacca agaagagcaa aaattgccta taaagcaagt gaaatattaa
    16501 gtttagaaaa tgatatagaa aaagctggtg aagtatcaag tgaagaactt tcatttggaa
    16561 gtaatataag aggaagtaaa gaatacagaa aagatatggc aaaagcctta gtagtgagaa
    16621 tgtataatag tattggaggt gagtgtgatg gtaaataaag aggtaagaaa aatagatagt
    16681 gaagccattg ttactggaaa accaatatat acagaagatt taatttttca taaagatgtt
    16741 ttaacaatta agcttcttag aagtccgcat gcttttgcga agataaaaaa catagataca
    16801 aaaaatgcat taaaggtaaa aggtgtagta aacatattca cttacaaaga tgttccaaat
    16861 tatagatatt caatggttgg agagtcatat ccagaagcat cacctcatga ccaattaata
    16921 cttgaagacg tagttagata tgtaggagat gaagttgcta taatagccgc tgaagatgaa
    16981 agggctgcta ttaaagctat gaaacttata aaagttgaat atgaagtaat gactcctata
    17041 ttggatgcaa gagaatcgga aggaaataaa attttaatcc atgaagaaga tgatttgttt
    17101 tgtccatttg attttggatt ggatgctaca aaaaatattg tatcgagaga aaaaatgggt
    17161 aaaggggatg tagaaaaaga gtttaaagaa agtgatgtaa tagtagaaca ttcttatatg
    17221 actcaatcac aagcccattg tatgatggaa actcatagag cttatacgta tatagatacg
    17281 aatggcagat tagtagtaac ttctgcaaac caatcagctt atcatatgag aagacaggtt
    17341 tctaaagcac tgggaatacc tctttcaaaa gttagagtaa taaaaccaag aataggtgga
    17401 ggttttggtg gaaaaaacat agctgtaaca gagatatatg catcatttgt aacttggatg
    17461 acaaaaagac cagcaaaact aatttatact agagaagaaa cttttgcaat gactaatact
    17521 agacatcaga tgttttttga tataaaaatt ggggcagata aagaaggaaa cataaaagct
    17581 atagatatga aagcattgaa taatacaggc gcttatggtg ataatggacc ttcagtttgc
    17641 tctgaatcag gtcataatgt acttccaaca tataataatg tacctgcaat aaaatttgat
    17701 gggagaactg tatatactaa tttagttcca ggaggagcat taagaggata tggagcaaca
    17761 caaggtacat ttgcacttga ttgtgcaatt gatgaattag cgcataaact taatatggac
    17821 ccaacagaaa taaagttaaa gaatattata aaaaaacaaa ctgaaggcgg aataatgaat
    17881 tttccaataa gaagttgtgc acttgatgaa tgtataatta gaggtaaaaa acttattgga
    17941 tgggatgaaa agtaccctgt tagggatatt ggaaacaata aggtgagggc tgttggaatg
    18001 tctgtgggaa ctcatggttc tggtatagcc aacatagaca tggcaatagt aaatatgagg
    18061 atggatgaag atggaactta taagttgttt tctgggtctt cagacttagg aactggttct
    18121 gacacaatac ttgcacaaat agctgcaaaa gcattgaata catctattga tagaatttcc
    18181 gtttatacag gagatacaga tatgtgtcca tatgatactg gtgcatacgc ttcttgtact
    18241 acttatgtaa ctggaaatgc tacaataaaa tctgctgaaa gtttaaaaca aaagatatta
    18301 aaagtagcac agaagaaact cgatttacca atagatgatt taatactata tgaagacaga
    18361 gtttgttata gtgaagatgt agagaagttt gttttgttaa gtgaattagg aaaagaatct
    18421 gttggtggtg gaaatcaaga agttttatat tcatctgaat cttatggtat acaagagagt
    18481 gctaaaccat tcttagcagg atttgctgaa atagagttag ataaaacaac aggggagttt
    18541 aaagtaataa attatgcttg ttctacagat tgtggtacag ttatgaatcc tgcacttgct
    18601 agaatacaag ttgaaggtgg tgtaactcaa ggtatagggc ttgcaatgta tgaagaccct
    18661 agacatggtt ctgatggaaa gcttcaaact tctaatctat tacaatacaa agttccaaca
    18721 aaaaaagata taggaaacat aattgttgat tttgtagata attatgagcc aacaggtcca
    18781 tatggagcta aatctttagg agaaatagtt attcatacac ctgcaccagc aatagcaaat
    18841 gcaatataca atgccacagg agttcgtata agagaacttc ctataacttc tgagaaagtt
    18901 tatatggcca tgaaaaaatt aaatcaggga ggtaaataaa tgattgctat taaaagtaaa
    18961 tacttaatat cttctgctga tgaaatatat acaaattctg ctgctgtagt agaaaatgac
    19021 attataaaag atatattgcc aaatgaagaa gttgaaataa aatataaaaa tattgaagaa
    19081 ataatagata aatcagatgc tataataatg ccaggattcg tcaatggtca tatgcatcaa
    19141 tatggagttc tttctagggg aataccggca aatgtacatt ttactgactt tgaaggtttt
    19201 ttaaatgact attggtggcc atttatagaa aatagaattg gattgaaaga agtgaaggca
    19261 acaactaaag catcagcaat agaacttata gaatctggtg tggtagcttt ttgtgatacc
    19321 ctagaagctc caaatacaga agaaggaact ttaattgagc aaggaaaaat actagaagaa
    19381 ataggcatga aagcgatttt atccttagaa agttgtgaaa gaataagctt tgaaaatgga
    19441 cttagatgtt tagatgaaaa ttctaattta ataaagtgga gtagagaaaa tagtaaatta
    19501 ataaatggaa taatgtgtac acatacaagc ttcacatgtt ctgatagatt tattaagaaa
    19561 gctaaagaag atgcaaaaaa attaaatgca ccaatgcaat ttcatttatg tgagagtata
    19621 tatgagccaa actatgctga aaaacatttt ggcaaaaaag cagttgacta ctataatgat
    19681 ttagatatat tggatgaaac tgtattagca tctcagtgtg taaaagtaaa tgatgaagaa
    19741 atagatatat taaaagaaaa aggtgtaaaa gtggttcata tgcctcttag caactgtgaa
    19801 gtaggtggag gtttttctcc agtaccaaaa atgattaaaa aaggcataaa agtggcactg
    19861 ggtactgatg gatatataaa tgattttttt gaagttatga gaggtgcatt tttaatgcat
    19921 aaatcagttg aagaagatgc atcagtaatg ccagcaaatt tagtatttag gatggcaaca
    19981 gagcatggtg cttatgtatt aggacttcaa aattctggta aaatagcagt tggtaataag
    20041 gctgatataa tagttatgga agatgaattt aaaactcctg tgactcttga taatatattt
    20101 gaccagatag tagttcaggg taaaaaagaa tttatatcaa atgtatacat agatggtaga
    20161 catattttaa aagaaaaaca gttagttgat ttagataaaa aagctattgt taaagaaatg
    20221 aaagaagttg cttgtgaatt ttggaaattt taacgaatgt aaatatatat gaaaagggga
    20281 agcagataat gtataatttt ttaggtaaag aattaaaatc accacttata ataggttcag
    20341 gaccattaac atatagtgca gctggatgca agatattaag tgatgcagga gcaggggcag
    20401 ttgtgactaa gactataaga aaagaaagag ctataaatcc agcaccacat atggttagaa
    20461 atacagctaa tgctctttta aataatgaaa aatggacaga ttttgagcca gagcaatgga
    20521 tagattttga aataccacaa atgaaaagag atgggactgt ctgtatagct tcagtaggtc
    20581 atacaattga agaaagtagc gaattagtag agaaagttgc aaatgcagga gctgacttta
    20641 ttgaattagt atcttatgac tatagagatt tgataccaat gcttaaagat gcaaaggaaa
    20701 gagtaaatat acctgtaata gttaaacttc ctcctatgat agatgagata ggcgattttg
    20761 ctaaaaaatt agaagaagca ggagcagatg caataactgc ttgtgactct gtaggaccag
    20821 catttagaat agacatagaa acagggcaac ctttattagg tggaaatgga ataggatatt
    20881 taagtggaga aactattaag ccaataactt tacagagaat atatgaaata aaaaaacaag
    20941 ttaatatacc aataattggc ttaggtggat gtgtaagtgg agatgatgcc ttagaaatga
    21001 ttatggcagg tgctgacttt gttggtatat gttcagtagt aattttaaaa ggcgcacaag
    21061 taatatctaa aatacatgat gatttaaaat ctaatcttaa tagattagga tataatacta
    21121 tagaaaatgc ttgtggtata gtacataatc atatgggaga tgttaatgaa agacttagct
    21181 ttgagtttac ttatgatgaa gaaaaatgta ctaaatgtaa gatgtgcgag agagtttgtg
    21241 catatcaagc gagaaaattg aatgataaga tggaattgaa tgaagaagag tgtagatatt
    21301 gtggactttg tatatcagtt tgtaaaccaa aagcacttgg aagaaaaatt tcatgttcag
    21361 agctgaatgt atagattatt ttataaaaaa caaagtttta aataaaaata agaaaggcta
    21421 tctcaaagag ataagccttt tttgttgttg accttatttt tgtttatttg tagttatttt
    21481 agatattaat tagttttgaa gtaataaagt gaatcaattt tgtttaatta aaattttaat
    21541 aattctgata tatatgttaa cattgtatag catttgtgta aaatgttata ataattaata
    21601 gggatggtga tgcaagtgca agagaagatt ttagagttat tatatactat aaatgaagat
    21661 gtagttagca taaaaaatga tatgtcggaa gtaaaacaag aaatgactgt aatgaaagaa
    21721 gatatatcag aagtaaaaca agaaatgact gtaatgaaag aagatatgtc ggaagtaaaa
    21781 caggagataa atgtattaaa aggagatatg tcggaagtaa aacaagaaat gactgtaatg
    21841 aaagaagata tgtcggaagt aaaacaggaa ataaatgtat taaaagaaga tatgtcggaa
    21901 gtaaaacagg aaatgattgt aatgaaagaa gatatgtcgg aagtaaaaca ggaaatgact
    21961 gtaatgaaag aagatatgtc ggaagtaaaa caggaaatga ctgtaatgaa agaagatatg
    22021 tcagaggtaa gacaagagat aaatataatg aaagaggata tatccgaagt aagacaagaa
    22081 atgactgtaa tgaaagaaga tatgtcggaa gtaaaacagg agataaatgt attaaaagaa
    22141 gatatgtccg aagtaagaca agagataaat ataatgaaag aagatatgtc tgaagtaaga
    22201 caagaaatga ctgtaatgaa agaagataca tcagaggtaa aacaagagat aaatgtaatg
    22261 aaaaaagaca tgtcagaagt aagacaagaa ataactgtaa tgaaagaaga tgtttccgaa
    22321 gttaaatcag aagtaaactt tatgcaaaat aaaataaata acattaatga agatatgaat
    22381 ggaattaagg acgaggtatc catagcaaat gaaaaattat atggaattga aattaagatt
    22441 gatagtctag aatgtgaaga taaatcaatg aaagagatac agatagaaca gaataatatt
    22501 ttaggaagtt tattacataa ttctgaaata aataaagcta ctcatgataa tatagaacat
    22561 aacatagcat atataaaagg tgacactaat tctataaaag aggatatagc tgaaataaga
    22621 agagacttaa atttagtaga gttagccact agtaaaaatt ggag
//
LOCUS       DAEPLF010000041        22440 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEPLF010000041 DAEPLF010000000
VERSION     DAEPLF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22440
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.041"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     repeat_region   561..917
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=561..589
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            1156..1344
                     /locus_tag="KR314_001404"
     CDS             1156..1344
                     /locus_tag="KR314_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327795.1"
                     /translation="MKIITDSSRADYFKQRRQNKKTFSVLLDREKVEKIEEHLKKQNK
                     TKTIWLEEKINEELEKEE"
     gene            1463..2329
                     /locus_tag="KR314_001405"
     CDS             1463..2329
                     /locus_tag="KR314_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HBH0327796.1"
                     /translation="MKNLIVKEFNGSQIYTFMWKEKSCWIANQIVGLFDYADVSKTIQ
                     DCIKAEDFEIEQEYDVLKGNEFNDFVTTLNVVANNIISNKARSITIFYEDGLYGFLQY
                     TDKPIGVQFRKWLRREVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLK
                     ELLDGAGFDNKSKLLTAKTLYKKAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPA
                     QLAVCEIIKKIDLEENEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPTKIAG
                     EKKNYHVVYKIE"
     gene            3194..3721
                     /locus_tag="KR314_001406"
     CDS             3194..3721
                     /locus_tag="KR314_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBH0327797.1"
                     /translation="MREGKRRRGCLFWFILIILFSGVVGAIAGNSTNNESTEKQKEDL
                     TKYIGEEGSIGDLKLTVNSISKASEISVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPDDSKYVPSLLIDVGNKYITMDTVNPNLDVTGNIAFEIPKDLLVSDCK
                     LKYSGSEQEFILKEK"
     gene            3859..4626
                     /locus_tag="KR314_001407"
     CDS             3859..4626
                     /locus_tag="KR314_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HBH0327798.1"
                     /translation="MGLFGKKKADICCICNTEIGVLNIEDGWICNSCFKEYCDALSMT
                     KAPKILRKLDIEKTISSTKKNNELQKIFNTTNNIENYIEFDEDNKKWLVSKKSINDKK
                     TPIIHSYEDIVKFELLENGETVTKGGIGRALAGGILFGEAGAIVGGITGKKTTRKVVD
                     TFKIKITINNIDNPIEYIELINKKTKTNSSAYEKAYKDAHKILSTLSAITQSIKETDN
                     INTKSVADEILKYKNLLDMEAITQDEFNTKKKELLNL"
     gene            4692..7064
                     /locus_tag="KR314_001408"
     CDS             4692..7064
                     /locus_tag="KR314_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBH0327799.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTTSINTTINSLERLQQRLHNPLN
                     TGGIQASQQSLNNIENILTRIEQKIGRNTNEQENFNNKIKQGSEAGSLLVSKLKSLAG
                     IYIGIRGIESITKAADTIASTKARLNLMNDGLQTTEQLNKMIYLSAQSARASYADTAA
                     QVAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPVTFSQALTMMKNDAYMIFGQTLGKISGALQSVRFSEIVVSIRNVMIAISSNIYDT
                     LNIIKNILNSEFFSNLVQGFTFCAVVITKSLGSIVNTALNIVNIFAQNWSIIKPIVFG
                     VASVFILFRGVLLATKIATIGNVIANVAHAASSSLSALMTNIQAAALMRSNGATLSAT
                     IATWGLNAALLACPITWVVLGILAFVVVVFVAVAAVNKFAGTSLTALGVIVGAVFAAV
                     AAIQNVMIWLLNGCIAVNEGITNGWNQCVYLMKQAIAKGVIFIIEKMASLNNSVNNAG
                     NALGKAFIDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYIKQQIDSL
                     NRWVGDAPEKVKLERMGYKDIGAAFQKGNALGTKWQNAITDKFKDTFDINKMLEDAKK
                     KLGLDDLWNKQNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQ
                     ETINRFTGVNIKIDMNNTNNISKDTDVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            7081..7770
                     /locus_tag="KR314_001409"
     CDS             7081..7770
                     /locus_tag="KR314_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBH0327800.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVLAPPKNETGRPNVKIEPK
                     RVDSVNATNTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKNLSKY
                     TIYKGQVLKLG"
     gene            7763..9655
                     /locus_tag="KR314_001410"
     CDS             7763..9655
                     /locus_tag="KR314_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH0327801.1"
                     /translation="MVDELVLANDRDVRLVIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKILAKDFNLKYNVIEDTKYKISRVEENKTLFDMILTALDDTLRE
                     KKEMYTLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSAHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSIMKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLNKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSNTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            9669..9929
                     /locus_tag="KR314_001411"
     CDS             9669..9929
                     /locus_tag="KR314_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBH0327802.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKMGDKLVLIRMQGGQQYLILDRM"
     gene            9934..10353
                     /locus_tag="KR314_001412"
     CDS             9934..10353
                     /locus_tag="KR314_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBH0327803.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            10354..11403
                     /locus_tag="KR314_001413"
     CDS             10354..11403
                     /locus_tag="KR314_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBH0327804.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATHTIAKGVFNIDIPLDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGKLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYRDEVNKIQDVGGVKVYPTWDGGGTVKLVIINSNFKV
                     PSEELVNLVQEEIDPIGHQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            11396..12013
                     /locus_tag="KR314_001414"
     CDS             11396..12013
                     /locus_tag="KR314_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBH0327805.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            12025..13050
                     /locus_tag="KR314_001415"
     CDS             12025..13050
                     /locus_tag="KR314_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH0327806.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTELKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNINNIELKIDPTIIFATLKDIQDLDAK
                     ISNVNTKIDTTKTELTSNIETAKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     IDDWIDNQEGGFKATVTHGLLTQRITVSIIDATTKDNVVPDFTIIDDNSIEVRSGVKV
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIHEEIGKVAEQLTGINSNIISTV
                     NNDILPI"
     gene            13067..14593
                     /locus_tag="KR314_001416"
     CDS             13067..14593
                     /locus_tag="KR314_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327807.1"
                     /translation="MALSMSYFNLPDKRKYTKNLAFNPFAGGRQNFEWTGGDHGLNGE
                     FKETCLSCTYNGSTLNWGSGNVWVLGEYGQYTFTYNCESMHVDTQQKFPYTSNRIITI
                     KGRPVISGSDTSLGNKRKGFSVDFTVSDDTPNVNLIVRAYLDDKLIQNITPVVQNSTL
                     TATVTDSQLNSLSVDGNHKLKIQLNDGYDNFDRIFTFKKIEKGIDISTSLVTDSQAKF
                     TVTKIYSELTKIECYLDETLKETFTTDLYSEKTINYELIDNAIHTLKIVVTDAENVVE
                     EKVISISKNIMPLQPDATLQDISTKLTEIGQGVRNGKTSIINTLALKNIDASLNNTLV
                     ELSEKIKGGFDSGDASLQDLMNQLTQANNTISQLNTKYKVASGTVTSFADSTKIAYPY
                     LTDNVTKPGSWIKVSNLGFKPNIFFADFDYYDAEYKNNYKLFLFACNGVATQRGVDFS
                     SVTSFIRKSGDEYFHANGWLYSNSEGDVYFNNTGVQIPAYNFDSTQKHTYKWYAIKFI
                     "
     gene            14608..14901
                     /locus_tag="KR314_001417"
     CDS             14608..14901
                     /locus_tag="KR314_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327808.1"
                     /translation="MNVPNRIIYDQTGRTIFETGESCGDVLPHYTITELHYIDIEYGS
                     IDYTRNRVIGINIETKEPILEEIPVYITDEEKRIQELENQLLIAENEKVGGLL"
     gene            14901..15083
                     /locus_tag="KR314_001418"
     CDS             14901..15083
                     /locus_tag="KR314_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327809.1"
                     /translation="MNINNVVVRILAERILSKGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGAEPTI"
     gene            15117..15683
                     /locus_tag="KR314_001419"
     CDS             15117..15683
                     /locus_tag="KR314_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327810.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPTTSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITINS
                     NKTGFSVHSYDTSYESYPFTGYYIACFA"
     gene            15714..16004
                     /locus_tag="KR314_001420"
     CDS             15714..16004
                     /locus_tag="KR314_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327811.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNNSNRKIQDETETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKIEASTKQAHLRIDEIVNRIGGK"
     gene            16008..16265
                     /locus_tag="KR314_001421"
     CDS             16008..16265
                     /locus_tag="KR314_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBH0327812.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            16526..17389
                     /locus_tag="KR314_001422"
     CDS             16526..17389
                     /locus_tag="KR314_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HBH0327813.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            17468..18280
                     /locus_tag="KR314_001423"
     CDS             17468..18280
                     /locus_tag="KR314_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0327814.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            18374..20773
                     /locus_tag="KR314_001424"
     CDS             18374..20773
                     /locus_tag="KR314_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0327815.1"
                     /translation="MIFKKYIKSLLIVVSVTLIMNKSILFVNALDLESNNNIKSYKRK
                     NVKEIDISNKFVDESFRRDIVEKVLDNRDDPLTNEVASKDNYKIYESDLNKIESCLEL
                     DLHKNPDVSNPNKIDNLDGIENFRNLEKLDCSSNNLESVDLSKNIKLKYLDISGNKIL
                     NIDLKNNNLINELHCDANKLSNLDLKNNKNLVSLFFTSNELTEIDLSNNVNLENLQFL
                     NNNLKSIDLSHNKKLKSLNCTNNKLKSLDLSNNVFLKNLSCSGNNLSKLNIKGLTSLE
                     YLGCAKNKLQDLDVSTNIKLDSLLCGKNSLTKIDISKNKELNSLECSGQYIEKSIIKN
                     GENIVVDIGIENSKLKELYPQNYNISTGFLTWENLSDFPNSISYSLEYEYGPVNDTET
                     MDVNISIDKLVKCRIRYIDYDGSIINEKEISDNSSINEIQINPQRENYEFSGWRNNKD
                     EKIYTSEQVSKLVFDDSTDFIATYKRKSTGSSGSSGGTSSKEDFNIAPSKEKITGADR
                     NETSVKISQKGWNKADNIVLINDSSISDALSATPFAKSKDAPILLTKNNNLNKLTEKE
                     INRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRISGNDRYETSIKLAKELDKNSNL
                     SKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQNDDMKDIKDLIANKNISKSYVIGG
                     ESLFNNKEVNNTLPSVTKIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLV
                     DALSVGVLAGKTESPVVLVGNGLDDSQKELIKNKKFKNITQIGGNGNEKAFNEVENLV
                     K"
     gene            complement(20814..20999)
                     /locus_tag="KR314_001425"
     CDS             complement(20814..20999)
                     /locus_tag="KR314_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBH0327816.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            21694..22371
                     /locus_tag="KR314_001426"
     CDS             21694..22371
                     /locus_tag="KR314_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH0327817.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSRLYFDLSTPGPNHNTKIDINPPS
                     FEKPLKK"
BASE COUNT         9128 a         2457 c         3985 g         6870 t
ORIGIN      
        1 taggtattta tttacttgaa tttcattgtt tatataaaaa aaatggaaaa atatgtaata
       61 attataggtt ataataattg tagcaaggat aataatcgaa agtgcgaagg gtgattattt
      121 tcatattaaa cgccaaattc caaataagga aggaggtgaa attatatgat aggtttttta
      181 ttaagcatac tagctggtgt tatatcagct tatatttatg acaaaataaa aaatcaccca
      241 gacgccaata agggtgattt aaaaaaataa tattttcact taacaactga aaataatcac
      301 tctttgtagg aataaattat ttccttgctt ttattatacc acaaattggt acagatattc
      361 aaaaataata tttttatgat ataataaaaa tgtagagatt ttgcagtgag cgatatttgt
      421 gataaattga agtttaacag ttgcaatata aggcgttgag ggtgtgtgat aaatgttatc
      481 aattgcacta ctcatggttc actgcaaatt taagagagtt gtatatgtgt aggtattgaa
      541 aatactcaac ttattttggg gttttagatt aactatatgg aatgtaaatg ttgtaagaag
      601 tatcattcta ttttttaatc tttctgtttt agattaacta tatggaatgt aaatttcagt
      661 gagaataagc tttattgtcg atgtaacact cgttttagat taactatatg gaatgtaaat
      721 agtacatata atgagtcttt aacatcagtt atgaaaggtt ttagattaac tatatggaat
      781 gtaaatgatt gtactttagc gtctgcacta gctttgtcta tcgttttaga ttaactatat
      841 ggaatgtaaa ttattttaca gatgaacaat tacagttact tcttgaatgt tttatattaa
      901 ctatgtggat tcaaaattaa atagaaaaaa gaaagcactt acttaaatag taggtgcttt
      961 tgttttgctc aaattactta attataaagt tgctaagtac aactttttag aattatacaa
     1021 tataaaaatg cactaaagtg attgattttg ctcaagttgg tcggttgagt aaaataatta
     1081 gaaaaaaatg ttaaaaaact cttgaaaagt gtcgcgatac aatgtataat tatattatcg
     1141 cgatacagaa aagaggtgaa aattattact gatagcagta gagcagatta cttcaagcag
     1201 agacgacaga ataaaaaaac ttttagtgtt ctactagata gagagaaagt agagaaaatt
     1261 gaagaacatt taaaaaagca gaacaagact aaaactattt ggcttgaaga aaagattaat
     1321 gaagagctag aaaaagagga ataaaaaata agagacgttc tccccgacca aagattgaac
     1381 atcccttatt gacgtatatt atatacacta actatagtat acgtcattcc ttgaaaaaat
     1441 tcaattaagg agtgtaatag ttatgaaaaa tttaatagta aaagagttca atggaagtca
     1501 aatttatact tttatgtgga aagaaaaatc ttgttggata gctaatcaaa tagttggatt
     1561 attcgattat gctgatgtat ctaaaacaat acaggattgc ataaaagcag aagactttga
     1621 gattgaacaa gagtatgatg tattgaaagg aaatgaattt aatgattttg taactacttt
     1681 aaatgtagtc gcaaataata taattagtaa taaagctaga agtataacta ttttttatga
     1741 agatggttta tatggatttt tacaatacac agacaaacct attggtgtac agtttaggaa
     1801 atggcttaga cgagaagttt taccaagcat aagacaaact ggtgcataca taactaacaa
     1861 tgctaatccc gaaaaactaa gagaaaaagc aagtgagatt gagaaattac agttagctta
     1921 caatagtaca tctatgttaa aagaactatt agatggtgca ggctttgaca ataaatccaa
     1981 actattaaca gctaaaacat tatataagaa agcaggaatt gatttaccaa tagagataaa
     2041 cgaagaagaa cattattttg atacaaagca aatagcatct aaactgaaaa tatattctaa
     2101 gagtaataaa ccagcacagt tggctgtttg tgaaattatt aaaaagattg atttagaaga
     2161 aaacgaagtc aaaggcgttt gggaaactaa tggttcttgg actggtactg taaataaata
     2221 tacaaagagt gtaatagata aggttagaaa ttggatagag gaaaataata gacctactaa
     2281 gattgcaggt gaaaagaaga attatcatgt ggtttataag attgagtaaa tttataagat
     2341 tgtattaaat aatttagttt agttttgggg ggattaatac aatgcatgag aatttacttg
     2401 atagtataga tattgaaaag agaaaagaag aatttagaat taaacttttg aaaataagag
     2461 aaacagatat agatatatat aataagatag aaagtatagt atataaactt tcttagaaaa
     2521 aattagagaa aaataattaa ataaatagat aaagcacttg gatatttcac tgtttcaagt
     2581 gctttatatg gtaaaaaatg gtataataaa atataggaat tttgcagtgt tcgatttttc
     2641 tactcaagta tagtttaaca attggaatac aaggcattga gaaggtttga taagtgttat
     2701 caattgcact attacccgct cactgcaaat ttgagaaagt tatatgtgtg taagtcttgg
     2761 aaatacacaa tttattttgg ggttttatat taactatatg gaatgtaaat ctttgtaatg
     2821 gtagtgtatt taagattgaa acatcaagtt ttatattaac tatatggaat gtaaatcaac
     2881 attagtagtt gtctttatac acatagcatc acgttttata ttaactatat ggaatgtaaa
     2941 tacagctccc aagacataca acgaatctgt aacatcagtg ttttatatta actatgtggt
     3001 atgtaaaaga cttatttaca gctttatttg ctaaatcaga aggttttata ttaactatgt
     3061 ggacttaaaa ttaaaaataa ttaaaaaaca cttactaatg taggtgtttt tttattgaaa
     3121 ggaagtgatt ataatgtaaa aattttgaag atatagtata atattcttat aaaatataat
     3181 ttagggggat attatgagag aaggaaagag aagaagagga tgtctatttt ggtttattct
     3241 tattatttta ttttctggag ttgttggagc aatagcaggg aacagtacta ataatgaaag
     3301 cactgaaaaa cagaaagagg atttaactaa atatattggt gaagaaggta gtataggaga
     3361 tttaaaatta actgttaata gcatttcaaa agctagtgaa atatcagtag catctggtta
     3421 tttagcatac actccagata gtggcaaata tggtattata aatttaacaa taaaaaatca
     3481 aactaaggaa agccaatctt ttatgctaaa ttcattcaca ttaataggac cagatgattc
     3541 aaaatatgtt ccatctttat tgattgatgt aggaaataaa tatataacta tggatactgt
     3601 aaatcctaat ttagatgtaa caggtaatat tgcatttgaa attcctaagg atttactagt
     3661 ttctgattgt aaactaaaat atagtggttc agagcaagaa tttatattaa aagaaaaata
     3721 atatatagat ttaaaaatat gaagcattta ctttaaataa gtaagtgctt ttttaattaa
     3781 aaggaggtga taataatgta aaaattttac ttatatagta taattacatt ataaaattat
     3841 tgtactgggg ggaatcttat ggggttattt ggaaagaaaa aagcagatat atgttgtatt
     3901 tgtaatactg agataggagt actgaatatt gaagatggtt ggatatgcaa ttcttgtttt
     3961 aaagaatatt gtgatgcact ttctatgact aaagcaccta aaattttaag aaaattagat
     4021 attgaaaaga ctatatcatc aactaaaaaa aataatgaac ttcaaaaaat atttaataca
     4081 actaataata tagaaaacta tatagaattt gatgaagata ataaaaaatg gcttgtgtct
     4141 aaaaaaagta taaacgataa aaaaactcct atcattcatt cttatgaaga tatcgtgaaa
     4201 tttgaacttc ttgaaaatgg tgaaactgta actaaaggag gaataggaag agctttagcg
     4261 ggaggaattt tatttggaga agcaggagct atagttggag ggataacagg taaaaaaaca
     4321 acaagaaaag ttgttgatac atttaaaatt aaaataacaa ttaataatat tgataatcct
     4381 attgagtata tcgaattaat taataaaaaa acaaaaacta attctagtgc ttatgagaaa
     4441 gcttacaaag atgcccataa aattttatca acattatctg ctattacaca aagcattaaa
     4501 gagacagaca atataaatac taaatctgta gcagatgaga tattaaaata taagaatctt
     4561 ttggatatgg aagctattac acaagatgaa tttaatacta agaaaaaaga attattgaat
     4621 ttgtaatata ataaacactt actaatgtag gtgttttttt atatggaaat ttatgaaagg
     4681 agagtgagaa agtggcaacg atacaaactt caataaagat tttcgacgga atgacacctg
     4741 cttttaggaa tatgactaca tctattaata cgacaattaa tagccttgaa aggttacaac
     4801 aaagattgca taatccgctt aacacaggtg ggatacaagc atctcaacaa agtttaaaca
     4861 atattgaaaa catattaacc aggatagaac agaaaattgg aagaaataca aatgaacaag
     4921 aaaactttaa taataagata aagcaaggaa gtgaagcagg ttctctatta gtatctaaac
     4981 tcaaaagttt ggctggcata tatattggaa taagaggtat agaaagtatt acaaaagcag
     5041 cagatacaat tgcaagtaca aaagcacgtt taaatttaat gaatgatgga ttacagacaa
     5101 cagaacaact taataagatg atttatttat ctgctcaaag tgcaagagct agttatgcag
     5161 atacggcagc acaagtcgct aaacttggaa tattagcagg agatgctttt ggaagttcgg
     5221 cagaggtgat aaagtttaca gaacttatga ataaagcttt tgtaattgga ggaacatcag
     5281 caaatgaagc tagtgcggct atgtatcagc ttactcaagc tatgggtgca gggaaacttc
     5341 aaggtgatga atttcgctct ataatggaaa atgcaccttt attagccact aaaatagctg
     5401 atgcaatggg aaaaactaaa gaccaattaa aggaattgtc aagtagtgga gcaataacag
     5461 cagatgttat aagaaatgca ctatttaaag cttctgatga gatagaaaag aaattcgcaa
     5521 gtatgccagt cacttttagt caagcactta ctatgatgaa aaatgacgcc tacatgattt
     5581 ttgggcaaac cctcggaaag ataagtggag cattgcaaag cgttagattt agtgaaattg
     5641 ttgtatctat aagaaatgtt atgattgcaa tatcttcaaa catttacgat acattaaata
     5701 ttataaaaaa tattttaaat agtgaattct tttcaaactt agtgcaagga tttacatttt
     5761 gtgcggtagt aataactaaa agcttaggtt ctattgtaaa tactgcatta aatatagtta
     5821 atatatttgc acaaaattgg agtataataa aacctattgt atttggtgta gcttctgttt
     5881 ttatattgtt tagaggggtt ttgctagcga ctaaaatagc tacaataggt aacgtaattg
     5941 cgaatgttgc tcacgctgct tcctcttcac tatcagcact tatgactaat atacaagcag
     6001 cagcactgat gcgttcgaat ggagctactt tatcagcaac aatagccact tggggtttaa
     6061 atgcagcttt attagcttgt ccaatcacat gggtagtatt aggtattcta gcatttgtag
     6121 tagtagtttt tgttgcagta gcagcagtaa acaaattcgc aggtacaagt ttaactgcct
     6181 taggagtaat tgtgggtgct gtatttgcag cagtggcagc aatacaaaat gttatgattt
     6241 ggttattaaa tggctgtata gctgtaaatg aaggcattac aaatggttgg aatcaatgtg
     6301 tatatctaat gaaacaagct attgcaaaag gtgtaatctt tataatcgag aaaatggcat
     6361 ctctaaataa ttctgtaaat aatgctggaa atgcacttgg gaaagctttc atagatgggg
     6421 caaatatcgc aatacgaggt gtaaacaaat taattgacct aataaataaa ataccaggga
     6481 taaatattgg taaagtagga gaggcaacgt ttacgccagt taaggcagat aatagttaca
     6541 tcaaacaaca gattgatagc ttaaacagat gggtaggaga cgcaccagag aaagtaaaat
     6601 tggagcgaat gggatacaaa gatattggag cagcatttca aaaaggaaat gcacttggaa
     6661 ctaaatggca aaatgctata actgataaat ttaaagatac ttttgacatt aataagatgc
     6721 tagaagatgc aaagaaaaaa ttaggattag acgatttgtg gaataaacaa aatcctttaa
     6781 acaaccttgg tggatttggt ggagatttag gaaaaaatgt aaaggacacg gcaggcaata
     6841 ctgcaaagat ggcaaagact atggataaaa gtcaagagga cttaaaatac ttaagagata
     6901 tagcagagca agaaacaatc aatcgattca caggagtaaa cattaaaata gatatgaaca
     6961 atacaaacaa cataagtaaa gatacagatg ttgatggaat agttaatgtc ttaactgaaa
     7021 aactgaacga tgctatggtt gtatcagcgg aaggaatagt ttaggaagga gagtgagaaa
     7081 atggcttatg atttttacct agatggagta caactaccaa tcgcaccacc caagcttgaa
     7141 gtcaaagtga caaacaagaa caagacagtt gatttgataa atactggaga agtaaacata
     7201 ttaaaaaaag aaggattatc tgaaataagt tttgaagcag aatttacaca taataaacta
     7261 cctttttgta gaggtcaatt tagagatgtt caattctttt taagtaagct agaattacta
     7321 aaaactgatt gtaagccatt tcaatttatt gtatctcgtg agttaggtaa caaggtctta
     7381 tttaacacta atataaaagt atctcttgaa gagtatgcta tttcagaaga tgcagataat
     7441 ggctcagata caaaagttgc aataaaatta aagcaatata gagattactc aactaaaaag
     7501 ttagttcttg cccctcctaa aaatgagact ggtagaccta atgtaaagat agagccaaaa
     7561 cgagttgatt cagtcaatgc cacaaacact aaaacatata cagtaaaagc aggggatagc
     7621 ctttggtcaa tttgtcagaa acaacttggt aatggttcat tatataagaa agtatacgaa
     7681 ctaaataaat ctatgatgga taaggcaaat aagggcaaaa acttaagtaa atacactatt
     7741 tataaagggc aggtgttaaa acttggttga tgaattagtg ttagcaaatg atagagatgt
     7801 aagattagta atagctcatt gggaagattt ctacgaacct gctgtcattg atggtatcac
     7861 atgggagata gaaagacgag gtacaccttc taagttagag tttacaatag tcatggatga
     7921 tatactagag ttttgcgaag gaaattctgt aaggctgtat tataaaggaa taggcatatt
     7981 ttatggatat atatttcaaa agaaaagaga taaagaaaat cacattaaaa ttgttgctta
     8041 cgaccagttg agatatttta agaataaaga tacttatgta tatagtaata aaactgcaag
     8101 tgaacttgta aaaatcttgg ctaaagattt taatttaaaa tacaatgtca tagaagatac
     8161 taagtataaa atatctagag tcgaagaaaa taaaacactc tttgacatga tactaacagc
     8221 actagatgat actttaagag agaaaaaaga aatgtatacc ttgtatgatg attttggaag
     8281 aataacatta aagaatgttg catcaatgaa actagatact gtcatgaaca atgatgtaat
     8341 tgaggatttt gactataatt catcaataga tagtgatact tacacaaaga ttaaacttgt
     8401 aagagacaac gaggagtcag gaaaaaggga tgtatatatt gctcaagact cagctcacat
     8461 gagaagttgg ggaatacttc aaatgtttga tacagtagac aaaaacatga gtgaagcaga
     8521 gataaaacaa aagtgtgata tacttctaaa actatataat aagaaaacta agtcattaag
     8581 tttaaaaaat gcacttggag atattagagt gagagcaggt tgtttagtac ctgttttttt
     8641 aaatctagga gatattgaat tgcaaaatta tatgttagtt gagaaagtaa aacatacatt
     8701 tgaaaataat tcacatttca tggatttgac cttggttgat ggagacgaat ttgcttcata
     8761 ttcttcaagc tcatatagta gtggaaatac taataataaa gatgaaaaga aaaatggtcc
     8821 tgcacaaagt attatgaaaa aaaatacagg taaaaaagtt cctgctatat ttactgcata
     8881 ttatccagga aacaatgcaa tggaaggtgg aaaaacagat tgcaatggaa agccacttga
     8941 tgtaaaatca agaactgttg ctggtccaat gaatcgagaa ggagttaaga aaacttggta
     9001 tactgatgat tttctaaata aacatccagt ttttgaatat ggagataaag taaaaattat
     9061 acttcctggt actgcctatg acaacaaagt atatacagtt aaagataatg gaggaagaat
     9121 atatgttgaa acaaacggaa catatcatat agatatacta ttagctaatg ctagtgaatg
     9181 taaaaaattt ggtagaaaga atggctatat aattataggt ggagatgaag aacaaacata
     9241 tcaagttgaa ggtaataacc aaagtaatac aaataacaat tctaaagaag ataaattaat
     9301 tagtatagca aaaagtaaac tgggttgtaa ttatgtgtat ggagcagaag gtcctaataa
     9361 ttttgattgc agtgggttta ctcaatggtg ttataaacaa ataggtataa aaattcctcg
     9421 tactgcttct gcacaaagta aagcaggaaa agcagtagat ttaaaagata gaagcaagtg
     9481 gaaagcagga gacttattat gtagaattgg tggaggaagt agtaatcatg ttgtaatgta
     9541 cattggaaac aatcaaataa ttcattcacc acaaacagga gatgtggtaa aaatagagtc
     9601 tgttaattct tatagaaaag gaaaagcata cacacatgtg agaagattta tataagtgag
     9661 gtggcaatat gagccaagat ttattacaga taataaaaaa agctgcaatg gatgcagtag
     9721 aaacaagcaa cccaatgagg gttgtatttg gaacaataga aagtattagt cctctaagag
     9781 ttaagataga acaaaaacta tctattggtg aaatttttct aatacaaaca gatacattta
     9841 aaagatatac agataaaaaa atgggagata aattagtctt aattcgtatg caaggagggc
     9901 aacaatactt gattttagat aggatgtgat aaagtgttac caagtgataa tttagattat
     9961 gatattgaag atgtatcaat aattaatttt gatgtaagac aagagccaag taagacattt
    10021 aaacttcata tagaaaagtc taagattgat ggtatttgtg atgatgtaga agcattaaaa
    10081 caaaccattt ttttaatttt aaatactgaa aggtatgagc atcttattta ttctagaaat
    10141 tatggtgttg aattaaatga tttaattgga gaacctattt catatgtaat acccgaactt
    10201 gaaagaagga taacagaagc actaattcaa gatgatagga ttgaaaatat agataatttt
    10261 gagtttcaaa atataaaggg taaagtacaa tgtagatttt cagttcatac aaaatatgga
    10321 aatataaaag cagagaaggt ggtgagtgta taattgtttg agttaatgac atttgaaaat
    10381 ataattaaaa gaatgttaga tagtgtacca gatacttttg ataaaaggga aggttctata
    10441 atatataatg ctcttgctcc tgttgctata gaacttacag aaacatacat tgctatggat
    10501 gaattactag accaaacatt cgtagatact gctagttatt attatttaga gaagagatgt
    10561 aaagaaaggg gaataacacc acttgaagcc actcatacga ttgctaaagg agtttttaac
    10621 atagatattc cacttgattc taggtttaat ctaggagaat ataactatgt agcaattgag
    10681 agaatatctg aaaaaacata taaaatgaaa tgtgaaacta ctggacctgt atttgagtta
    10741 ggaaaactaa tacctattga atatatagat ggtcttgaaa ctgctgaact aactgaaatc
    10801 ttgataaatg gagaggatga agagtcagaa gatagtttaa gacaaagata ttatgatagc
    10861 ctaaattcac agagttttgg cgggaatata caaaactata gggatgaagt taacaaaata
    10921 caagatgttg gaggagttaa agtttatcca acttgggatg gaggtgggac tgttaagtta
    10981 gtaataatta actctaattt taaagtgcca agtgaagaat tagtaaactt agttcaagaa
    11041 gaaattgacc caattggaca ccaaggagaa ggcttaggat tagcaccgat tggacaccga
    11101 gtcacagttg aaggagttac aagtacaact ataaacatat cagcagaaat aacatacaaa
    11161 aatggctaca cttgggagaa tataaaatca attgcagaag aagcaataga cgactattta
    11221 aatgaactta acatgagttg ggaagatgaa gaaaacttaa tagtccgtat atctcaaatt
    11281 gaaactagat tacttagtat tgatggagta ttagacattg caaatacaat gataaatgat
    11341 gttaaatcta atctaacaat agatagtaac agcatagtag tgagaggtga ggtagttgga
    11401 taaagagatt aatctaataa attacttgcc acaaattcta caagataaag aagaatatat
    11461 aaaagtattt aatgtagaaa ataaagaaat aaaaacacta catgataaat taaaggacct
    11521 atcaaatgac cagtttttag aggacctaac tataagtggt ataaaaagat gggaaaagat
    11581 aatgtctata actcctaaaa gtaatgagag tttagaagat agaaggttta ggatttttag
    11641 taaatatata agtaaattac cttactcaga gagattttta aggaactggc tagataatgt
    11701 agttggagaa ggcaattatg aattaactat taataatgct acttataaca tacaccttga
    11761 gagtgatgct agaaatcaag attggtttga ggaagttcat tcttttgtaa gtaatattaa
    11821 gccatgtaat atgactttag attacactag agtgcttata agcaaagaca attatatgaa
    11881 ttttggtata acaaccctaa tgggtcaaga aataactata tacccttgga gtccaccaga
    11941 tatagaaact tatggagaaa ttgatgtatt aactggcaat ggagttggat accaagagat
    12001 aacaatattt taggaggtga tatattggct atagataaaa gttattacac tataattaca
    12061 gatgtaggaa aagcaaagat agcaaatgca agtgtcacag gtaataaagt agggtttgta
    12121 aaaattcaac ttggtgatgg tggaggaagt gagtatactc ctactgaaag tcagacagaa
    12181 cttaaaaatg tggtatggga aggcaacatc ggaaatacaa ccacagatga aactgcgcca
    12241 aactgcataa tattagagag tttaatacca tcaagtgtag gtggatttat gataagagaa
    12301 ataggatatt tagatgatga aaataatcta attgcaatct ctaaatataa agaatgttat
    12361 aaaccttcta tagaacaagg agcagttgtt gatatgaagg ttaaaactgt gcttattgta
    12421 tctaatataa ataatataga acttaaaatt gaccctacaa taatttttgc tacgctcaaa
    12481 gatatacagg atttagatgc taaaataagc aatgtaaaca ctaaaattga tacaactaaa
    12541 acagagttaa caagcaacat agaaactgct aagacagagt taaacactag aattgacaca
    12601 gaaaatgaga aacaaaatat taaaattgac cagctaatag caggtggttc aaatgttgca
    12661 tctactcaaa caataacaat tgatgattgg atagataatc aagaaggtgg attcaaagca
    12721 actgtaacac atggtttatt aacacagaga ataactgtaa gtattataga tgctactaca
    12781 aaagataatg tagtacccga tttcacaatt atagacgata attctattga ggttagaagt
    12841 ggagtaaaag ttgagttaaa tgtttatgtg attaatggga atgcagagac tcattttatt
    12901 aatgcgactg tagatgataa tagagtgtct gaaatgacta cttattcatc aaaaaagata
    12961 catgaagaaa ttggtaaggt agcagagcaa ctgacaggaa ttaatagtaa tattatatcg
    13021 actgtaaata acgacatact acctatatag aaaggagcgt gaaaaaatgg cgttatcaat
    13081 gagttatttt aatttacctg acaaaagaaa atatactaaa aatcttgcat ttaatccatt
    13141 tgcaggtgga aggcaaaatt ttgagtggac tggtggtgac catggactaa atggtgagtt
    13201 taaagaaact tgcttaagtt gcacttacaa tggttcaaca ttaaattggg gttcaggaaa
    13261 tgtttgggtc ttgggggaat atggtcaata tacatttact tataattgtg aatcaatgca
    13321 tgtggataca caacaaaaat ttccgtatac ttctaataga ataataacta taaaaggtag
    13381 acctgtaata tctggttcag atacttcact tgggaataaa agaaaaggat ttagtgtaga
    13441 ttttactgta agtgatgata ctccaaatgt taatttaata gtacgtgctt atcttgatga
    13501 taaattaata caaaacatta ctcctgttgt acaaaattca actttaacag caacagtaac
    13561 agatagtcaa ctaaactctt tatccgtaga tggaaatcac aaactaaaaa tacaattaaa
    13621 tgatggatat gataattttg atagaatttt tacttttaaa aaaatagaaa agggaattga
    13681 tatatcaaca tcattagtaa ctgatagcca agctaagttt actgtaacaa aaatatacag
    13741 tgaattgaca aaaatagagt gttatttaga tgaaacatta aaagagactt ttactacaga
    13801 cttgtattca gaaaagacta taaactatga acttatagac aatgcaatac acacattaaa
    13861 aatagttgta actgatgcag aaaatgttgt agaagaaaaa gttataagta taagtaagaa
    13921 tataatgcca ttgcaacctg atgcaacatt acaagatata tcaactaagt taacagagat
    13981 tgggcaaggc gttagaaatg gtaaaacaag cattataaat actttagcat taaagaatat
    14041 agatgcaagt ttaaataata cactcgttga gttatcagag aaaattaagg gaggttttga
    14101 tagtggagac gctagtttac aagatttgat gaatcaatta acacaagcta ataatactat
    14161 atcgcagtta aatactaagt ataaagttgc tagtggtaca gttacttctt ttgcggatag
    14221 tactaaaatt gcttatccat atctaaccga caatgttact aaacctggtt cttggattaa
    14281 agttagtaat ttaggcttta aacctaatat tttctttgct gattttgatt actatgatgc
    14341 tgaatataaa aataattata aacttttcct tttcgcttgt aatggtgttg ccacacaaag
    14401 aggtgttgat ttttcaagtg ttacatcttt tatcagaaaa agtggtgatg agtattttca
    14461 tgctaatgga tggctatata gtaactcaga gggggatgtc tattttaata atactggcgt
    14521 tcagattcca gcttataact ttgattcaac tcaaaaacat acttataaat ggtatgctat
    14581 taaatttatt taaaaggagg aaaaaacatg aatgttccaa accgaataat atatgaccaa
    14641 acaggtagaa caatctttga aacaggcgaa tcctgtggag atgtattgcc acattataca
    14701 ataacggaat tgcattatat agatattgaa tatggaagta tagattatac aaggaataga
    14761 gttataggta taaatataga aacaaaagaa ccgattttag aagaaatacc agtatatata
    14821 acagatgagg aaaagagaat acaagagtta gaaaatcaat tattaattgc agaaaatgag
    14881 aaagtaggag gattattata atgaatataa ataatgttgt agtaagaata ttagcagaaa
    14941 gaatattaag taagggatta aatcctctaa aaaatagaga atttcagtta gatgacgtga
    15001 ctaacacaga gtacagaaaa gctgtagagg attatattat aaaaaatagt ggagtagtag
    15061 aaggagcaga accaactata tagagggttc tttttttatt gaaagaaggt gactaaatga
    15121 cttttaaaga gttggttaat aaagttagaa atcttgtatt agaagcaaag aatgtaacta
    15181 tagaagatac agaaagtaaa tttacaagtg aaaatgtaga aggagcattg aaagaatgta
    15241 tagatagagc agatgaggct tttcaagaag ccgatagtgg aaaaacactt ttatcaactg
    15301 ctatcggctc tcctactaca tcagaacaaa catttcaaga ttatgcgaac tatattacag
    15361 gatttaagag caatataagc aatttagaaa ctcaattgaa aagcaaatat tctattagat
    15421 atggtcctat tgatggatat gatggtaatc ctttttctgc taattttggc aagagtgcaa
    15481 gttaccttat tgtctatgtt tactttagaa gaagtgtata ttattataat cccagtggta
    15541 gttctttagg aagtaataca ggaggttctg aacgtgcatg gattactata aacagcaata
    15601 aaactggttt ttcagttcat tcatatgata ctagttatga gtcatatcct tttacaggtt
    15661 attatattgc ttgtttcgca taataaatta tattattaaa actaggagga tgtatggaag
    15721 aaattagcat aaatctacta tgtgcagtag caggagttgt aatatcctac ttagcattta
    15781 gaaataactc aaacagaaag atacaagatg aaacagaaac aactacaaaa ttagaacaac
    15841 aaataacttt tttatgtgag aatgtaagag atataaagca tgatgtagca aagtttaata
    15901 ctagtttctt agatattagt gaacgagttg caaaaataga agcaagtaca aaacaagcac
    15961 atcttagaat tgatgaaatt gtaaatagaa ttggaggaaa ataagagatg gataatttaa
    16021 taagttttat accagagcag ttgctaattt tagtagctgc tctctctatt ataggtaaag
    16081 gttgtaaaaa atataaacaa ttagataata aatacattcc agtagtgtta ctgatacttg
    16141 gaataggttt ctcaatatgg atgctaggat taaatcctgt tgcagtctta caaggtgtaa
    16201 tttgttgggg agttgcaata ggtataaatc aaacttacaa acagttgaag gatggtgaaa
    16261 agtaatgaaa ttaacaaaaa tattattact cacaaaaatt taaaaaattt cattaaatag
    16321 agtatacttt tatagctaat aagtatataa taatagtaca gaactcgtaa agcatttata
    16381 tgctcaaata tacgggacat tgatttttga aggaacccgc caagcttctt ttatagctca
    16441 aataggcggg acagataaat ttacccacta atattaattg gtgggtttta ttatataaaa
    16501 gtatatctat gaaaaggtga aaacaatggt tgaagtaaaa gaagaaaaaa catttgatga
    16561 acaaatagat attttaaaaa gtagaggatt aataataaat gataaagaag atgctaaatt
    16621 tgtattaagt aatgtcaatt attataggtt tacagcatat cttctaagtt ttaagaatga
    16681 tgatggctca tataaagaag gaactacgtt tgaagaagtt tatgatatat ataggtttaa
    16741 taaggaattt aggatattat taacagattt gttagggagc atagaaatag catttaggac
    16801 atacattgca tatacattag caattaaaca tggtgcttgt ggatatctag aaagggagag
    16861 tttcaaagat gaaaaattct atattaattt tttgacagca ttagagagag aaaaaagtaa
    16921 taattcagat aagcttttta ttatacacca taaagaaaaa tatgaaggaa aacttcccat
    16981 ttgggttgca acggaaataa tgacttttgg tatgttgtca aaattatatt caaacatgtt
    17041 gccagaagat actagataca taaaaaataa tttgtgtaga gtgaatactt tattagttaa
    17101 atcttggtta caatcattaa cacaggtcag aaatcaatgc gctcattatg gtagaatata
    17161 taataataat ttccgtatta taacaataaa aaacgaatat aaaaagtata acttggataa
    17221 taaaaagata ttttcttata tacttgctat gaagcatttg actatggata aattaatttg
    17281 gaatagtttt tttataaaac ttcaaaagtt aattaatgat tataataatt ctatagactt
    17341 aaagcttatt ggttttccta ataattggat agagatattg gctaaataaa atagttactt
    17401 taagaagttt ataaacactt actatatgta agtgtttttt tattgaaaag aaggaggaaa
    17461 ataaataatg aaaatatgta ttacagtagg acacagtatt ttaaaaagtg gtgcatgcac
    17521 ttctgctgat ggagtagtta acgaatacca atacaataaa tctcttgcac cagtattagc
    17581 agatacattt agaaaagagg gtcataaggc agatgtaata atatgtcctg aaaagcagtt
    17641 taaaactaaa gcagaagaaa agacttataa aatacctaga gttaatagtg gaggatatga
    17701 tttacttata gaactacatc taaatgcaag tgatggtcaa ggaaaaggtt cagaagttct
    17761 atattatagt aataaaggtt tagagtatgc aactagaata tgtaataagc taggtacagt
    17821 atttagaaat agaagagcta aattagataa aggattatat atcttaaata gttcaaatcc
    17881 tacagcagta ttaattgaaa gtttcttctg tgataataaa gaagattatg agaaagctaa
    17941 gaaactaggt catgaaggta ttgctaagtt aattgtagaa ggtgtattaa ataaaaatat
    18001 aaataatgag ggagttaaac agatgtacaa acatacaatt gtttatgatg gagaagttga
    18061 caaaatctct gcaactgtag ttggttgggg ttataatgat gggaaaatac tgatatgtga
    18121 tataaaagat tacgtgccag gtcagacgca aaatctttat gtcattggtg gtgcagcatg
    18181 tgagaagatt ggttctatga ctaaagaaaa atttactatg ataaagggta atgatagatt
    18241 tgatacactt tataaggcat tagattttat taatagatag attatataaa atattttagt
    18301 atttcttgaa aatcataagt atggtaatat ttagataaat attatttatt attaataggt
    18361 atggggggag attatgatat ttaagaagta tataaaatca ttactgatag ttgttagtgt
    18421 tacattgata atgaacaaat caatattatt tgtaaatgca ttagatttag agagtaacaa
    18481 taacataaaa tcttataaaa gaaagaatgt aaaggaaatt gatataagca ataaatttgt
    18541 tgatgaatcc tttagaagag atattgttga aaaggtatta gataataggg atgatccatt
    18601 aacaaatgaa gttgcatcaa aagataacta taaaatatat gaaagtgatt taaataaaat
    18661 tgagagttgc ttagagttag atttacataa aaatccagat gtttctaatc caaacaaaat
    18721 agataattta gatgggatag aaaattttag aaatctagaa aagcttgatt gtagttcaaa
    18781 taatctagaa agtgtagatt taagtaaaaa tataaaatta aaatatctag atattagtgg
    18841 aaataaaata ttaaacattg atttaaaaaa caataatctt attaatgaat tacattgtga
    18901 tgcaaataaa ttatcaaatt tagaccttaa aaataataaa aatttggtaa gtttattttt
    18961 tactagtaat gaattaacag agatagattt aagtaataat gtgaacttag aaaatttaca
    19021 atttcttaat aataatttaa aaagtataga tttaagtcat aataaaaagt tgaaatctct
    19081 aaattgtact aacaataaac ttaaatccct tgatttaagt aataatgttt ttttgaaaaa
    19141 tttaagttgt tcaggtaata acctgagtaa attaaatata aaaggactta caagtcttga
    19201 gtatttggga tgtgctaaga ataaattaca agatttagat gtatctacaa atataaaatt
    19261 agattcttta ctttgtggga aaaatagttt aacaaaaata gatataagta aaaataaaga
    19321 attaaattca ctggaatgta gtggtcaata tatagaaaaa agcattataa aaaatggtga
    19381 aaatatagtt gtagatatag gaattgaaaa ttcgaaatta aaggaattat atccacaaaa
    19441 ttataatatt tcaacaggtt ttctaacatg ggaaaatctt tctgactttc caaatagcat
    19501 aagttatagt ttggaatatg aatatggtcc agtaaatgat acagagacta tggatgtaaa
    19561 tatatcaata gataaattag tgaaatgtag aatcagatat atagattatg atggaagtat
    19621 aattaatgaa aaagaaatat cagataattc tagtataaat gaaattcaaa ttaatcctca
    19681 aagagaaaac tatgagttta gtggttggag gaacaataaa gatgaaaaaa tatatacatc
    19741 agagcaggta agtaaattag tttttgatga tagtactgat tttatagcaa cttataaaag
    19801 aaaatcaaca ggatcaagtg gttcttctgg tggaacttca agtaaagaag attttaatat
    19861 agcaccatct aaggaaaaaa taacaggtgc agacagaaat gaaacatcag tgaaaataag
    19921 tcaaaaagga tggaataaag ctgataatat agtgttaata aatgactcta gcatatcgga
    19981 tgctttatca gcaacaccat ttgctaagtc taaagatgca ccaatattac ttactaaaaa
    20041 taataattta aataaattaa cagaaaaaga aataaataga ttagaagcaa aaaatgtgta
    20101 catagtgggt ggattaaagt ctgttgatga aaaagtagtg tctgatttaa agaaaaaagg
    20161 attaaatgta attagaatat ctggaaatga tagatatgaa acatcaataa aacttgcaaa
    20221 agaattagac aaaaattcta acctgtcaaa agtagtagtg gtaaatggag aaaagggact
    20281 agctgatgca gtaagtatgg gagctatatc agcaaaagag gaaatgccaa ttcttcttac
    20341 taatcaaaat gatgatatga aagatataaa agacttaata gctaataaaa acatatcaaa
    20401 atcatatgta ataggtggag aatctttatt taataataaa gaagtgaata atacattacc
    20461 atctgtaact aaaatagcag gttctgatag aacagaaact aactcaaaag taataagtca
    20521 tttctatagc aaagatacac ttaatgactt atatgtagct aagaatggaa tgaataaaca
    20581 agacgattta gtagatgcct tatcagtagg agtgcttgca ggtaaaactg aatctccagt
    20641 agtattagtt ggaaatggat tagatgatag ccaaaaagag ttaatcaaga ataaaaagtt
    20701 taaaaatata actcaaattg gtggaaatgg aaatgaaaaa gcatttaatg aagtagaaaa
    20761 cttagttaaa tagattgaaa ggtagtaact ggaaatagtt actacctcct tttttattca
    20821 ttttcagttt tttcaataat tatttttcca tcctcaaaag taacaagaac atccctttca
    20881 tcaggagaga tattcatctc tttaatccat gacattggaa gtatcaatct gggtgtaaaa
    20941 gagcctttgc cacttttatt aaatccaatt ctaagttttc tttgctccaa taatatcatt
    21001 cctttctact attaataaat gccagcaata ataaagtaaa taccaaagca ataagagctt
    21061 taaaagtaaa acttattatt atattaataa tatttaaaac tatagtgata ccaagaataa
    21121 tcatacaaat tctataaaat ttattattca tattaaatta gaaaatgtgt tataatatta
    21181 gaaagaagct acctagaagg aaggtatttt cctcctagga ttgaaactta taattcttta
    21241 atcaatctta gtattgcgag tactagatta attaaaacta atgtgaaaga gaaagcattt
    21301 ttaattttat taaatttgtt ttctctttta ttttttcttc tttctaatct ccccactttc
    21361 ttacctcctt tctatattta tattatatca tcacgtgacg tgaaagtcaa taaatattac
    21421 atatttttca ataaagttat attgataaac tatatttttt acaaataaaa ataaatggtg
    21481 acaaaaccaa acttgccacc attttgccac cgaaaataaa taatagaata aatttaaatt
    21541 aaacataata aaacataaac aacaaaactg accttaataa aacattgaaa taccaacact
    21601 tgtaaaaaat aacaacaata aacaaataac ctagaataat attacaactt aagtaaataa
    21661 taattttgaa taataattta cggaggtatt catatgttta aacacgataa agcattatta
    21721 aaagaagtaa aagtagaaag accaaatcct caatatgctg ttttaatgca agaacagttg
    21781 ggtggtgcaa atggagagtt aaaagcagct atgcaatact tgtcccaaag ttttagaata
    21841 aaagaccctc aaataaaaga tttatttctt gatatagcag cagaagagct tagccatatg
    21901 gaaatggtag cacaaactat aaatttatta aatggacatg atgttgatta taatgcagtt
    21961 aatacaggcg aaatagaaac acatgtatta actggtctat caccagtctt aataaactca
    22021 tcaggagctc cttggactgc taattatgtt acagttacag gggatttagt agctgactta
    22081 ctttcaaata tagcttctga acaaagagct aaggtagttt atgagtactt gtatagacaa
    22141 atagatgata aatatgtaaa agaaacaata gatttcttac ttaacagaga agaagcacat
    22201 aatgctctat ttagagatgc tttaaataaa gttaaagata caggttcaaa tagagatttt
    22261 ggagttacag aagattctag attatatttc gatttgtcta ctccagggcc aaatcacaat
    22321 actaagatag atattaatcc tccttctttt gaaaaacctt taaaaaaata atttgatatt
    22381 ttttatgttg ttttgtttta gtagttataa aaattattta ctagaagaat taattatatg
//
LOCUS       DAEPLF010000042        22315 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEPLF010000042 DAEPLF010000000
VERSION     DAEPLF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.042"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            211..1122
                     /locus_tag="KR314_001427"
     CDS             211..1122
                     /locus_tag="KR314_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBH0327818.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            1382..1816
                     /locus_tag="KR314_001428"
     CDS             1382..1816
                     /locus_tag="KR314_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327819.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            1839..3374
                     /locus_tag="KR314_001429"
     CDS             1839..3374
                     /locus_tag="KR314_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327820.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVIYK
                     IDCDVILVNLFELIINNCIFSTILGNEVINLEISKYEYEFLEKQFKNMYNNITEDKVI
                     GSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKTEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            3458..4825
                     /locus_tag="KR314_001430"
     CDS             3458..4825
                     /locus_tag="KR314_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0327821.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKARDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMNTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            4860..5084
                     /locus_tag="KR314_001431"
     CDS             4860..5084
                     /locus_tag="KR314_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBH0327822.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(5534..5932)
                     /locus_tag="KR314_001432"
     CDS             complement(5534..5932)
                     /locus_tag="KR314_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBH0327823.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMVG"
     gene            complement(5935..6336)
                     /locus_tag="KR314_001433"
     CDS             complement(5935..6336)
                     /locus_tag="KR314_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0327824.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLVAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            6695..7681
                     /locus_tag="KR314_001434"
     CDS             6695..7681
                     /locus_tag="KR314_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0327825.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLNEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            7665..8576
                     /locus_tag="KR314_001435"
     CDS             7665..8576
                     /locus_tag="KR314_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0327826.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            8689..10257
                     /locus_tag="KR314_001436"
     CDS             8689..10257
                     /locus_tag="KR314_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0327827.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHVLEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            10332..11357
                     /locus_tag="KR314_001437"
     CDS             10332..11357
                     /locus_tag="KR314_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327828.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDEYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            11350..12294
                     /locus_tag="KR314_001438"
     CDS             11350..12294
                     /locus_tag="KR314_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327829.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            12653..14311
                     /locus_tag="KR314_001439"
     CDS             12653..14311
                     /locus_tag="KR314_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0327830.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERDSKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASNGKSSW"
     gene            14292..15308
                     /locus_tag="KR314_001440"
     CDS             14292..15308
                     /locus_tag="KR314_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0327831.1"
                     /translation="MVKAVGDIIGKCSAISFDMKLDKSYDEVIVDFKNLINNIDNKDV
                     LLFTDMGSLNSFDEIIKKEKKCEVRVIPMVTTLTVLEAVQKANMGLPLNDVYNSITNT
                     RKYYFGTNEIQNKENLSKTIIIASHVSEGVDNKTRKILEEKMSRYLDGIDIISVPYKT
                     EKDLSLNITKLKESSNIVAVINEQRINIRGIDYISKKDIDKDENINKLKNIIKISIGY
                     DDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLVFNIEKKYDKVIGLMMHLAFMVDGLI
                     GNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDKKYNIEINEKECYQILLILEYAEIIE
                     KDYQ"
     gene            15523..16152
                     /locus_tag="KR314_001441"
     CDS             15523..16152
                     /locus_tag="KR314_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327832.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            16287..16613
                     /locus_tag="KR314_001442"
     CDS             16287..16613
                     /locus_tag="KR314_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0327833.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            16629..17918
                     /locus_tag="KR314_001443"
     CDS             16629..17918
                     /locus_tag="KR314_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0327834.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            17942..18271
                     /locus_tag="KR314_001444"
     CDS             17942..18271
                     /locus_tag="KR314_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0327835.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            18419..19747
                     /locus_tag="KR314_001445"
     CDS             18419..19747
                     /locus_tag="KR314_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH0327836.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            20064..20924
                     /locus_tag="KR314_001446"
     CDS             20064..20924
                     /locus_tag="KR314_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBH0327837.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            21129..22109
                     /locus_tag="KR314_001447"
     CDS             21129..22109
                     /locus_tag="KR314_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0327838.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVICGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         9026 a         2281 c         3938 g         7070 t
ORIGIN      
        1 atatgtaaat agtttttata aatttaatta tgaattagtc atcaataaac aagaaattta
       61 gctgtagaaa tccattataa aggctattat aaattttaat atgctaaaaa tataaaaaga
      121 atagttgaca gtatagaaaa tatggaataa actaaaagtg taatagtaat acgaaattat
      181 atattacatt gaaaggtgtg aatgattatt atgaataaaa aaatagaatt aatagggaat
      241 tttgcaagtg attggattag gtatagtgat tatgaatata aaattactga agaagatgaa
      301 ttatatatag ttccaacaga agatgctgtg tttaccatgt ataacccatt tgatgtagca
      361 gatgatataa tagtagatat aattcgcata ggacaagagg cattacaaga tatatcaaaa
      421 gagacaaaag aaatgaaact aaagaaagaa gtacttgaat ttgtaaaaaa gtatggatta
      481 ttgggtttaa tatattcaag tgtatataat agagatatag ttggagaaga aagagtactg
      541 atgattgaaa agaattatat tacaaaagag aatatattga gtacagatga atatgtaaac
      601 atgtttatac catttgcaag tgatgaagat gtgtatttta aaaaatatag aaggggtata
      661 gatgtatcaa agagagaaga atctccaaag ttttttggaa agagaccatt gattttagat
      721 ttagtatttt caaagttcta cactgaacaa ctagattgga ttgtaagttt tgctaaagat
      781 atgtgtaagc attttaatca aatttgtgta tataaaaata catctaatct attaactgaa
      841 agtgttacta ttatgtctgg agggtttaat cctcaaaaga taggattttc tataagccaa
      901 ttagataaac caattattgc atgggagttt gattctttga aaacagctat tgagactatc
      961 tatgcctttg caattacaag tgaaccttct tctttaaata aatgcaagta ctgtggtaaa
     1021 atttatattc ctacaaattc taaatctcaa tactgttcac cttcttgtag aaattgctca
     1081 aatgtaaaga aaagtagaag tagagccaaa aaagataaat aaaattaata aatatatcat
     1141 tgtttagatt ttagtaaata actactgaat taaaagttgt tatagaaaat tatatatcta
     1201 aatgatggta tttttttgtt gagaaaattg aaaagtttaa ttgagatagt aattttgagt
     1261 tgacaaaaac cacttttaac actactatta taagtggttg gtaaaataat atacactaat
     1321 ttaaatatag cataaaaaac aaaaaaagtc aatgaaaaaa ataaaaaagg agaatttaaa
     1381 aatgatatca cctattattt tgagttctat aaatcagaat ttaaaggaaa tagaaagaaa
     1441 tgaactgctt gaaactaata tagaatctgg agattatggt ctagctcttt ctgaatctga
     1501 tgtaaaagat ataataaatt cgagagataa tactttaaag gggtatggaa gaatagaatt
     1561 agatataaaa gttacaaaac agttaataga aaatatatat acatcacaat atacaaatgt
     1621 ggacaattat ttagaggcta ttaatgatat gcaagaaatt ttttattatt taaaaaatga
     1681 aactgatgac aagatatgtg atgatgagat tatagaaata ctgggtgaat tttatgaaaa
     1741 attttcaggc aatatggaca atgtaagagg agaagcagac gaatttgcaa agaaatttaa
     1801 atttggagaa gtttaatata tgataggaga gattttgtat gttaaataat ttaagtaata
     1861 ttaatataaa cgaaaataat ttaataaaga accaatattc catatctcta cttaaggaat
     1921 gtctaaattg taaagtgata gatgaaagag aagtatataa tatacaacaa gaaatatctc
     1981 taatacttat ggatttgatt aaaaaatata caaatggtca aagcacatca gtaaaaacag
     2041 aagttgccga aaaactgttg atttcaatat ggtatgctat tgatgcatat ataaataagc
     2101 ttggagatat agaaaaaaga gttgtggagt tgactgctaa gaatgttaaa aaagtgtacc
     2161 ttggaggcat tgaaatatta gaggaagagt tagttaggct gaaagatttt tataaactaa
     2221 tgatgagtag aaaattggat actaattcaa tagcatataa tgataccttg atggagttat
     2281 caagtttctt taaaagttat attgtaaaat ttgaagctca ggacgttcca gtaagtatag
     2341 attatccatt ggcactagat gatatggatg ttaagggtat ttattatgtg aagaattata
     2401 ttgagagtat agatatagaa aatagatttt gtaacctatt taagaaaaaa gatagagaaa
     2461 aattatttta tgactatggg gtgatttata aaatagattg tgatgttata cttgtaaatt
     2521 tatttgaact tattataaat aattgtattt tttctactat tttaggaaat gaagtgatta
     2581 atttagaaat ttcaaaatat gagtatgaat ttttagaaaa acaatttaaa aatatgtaca
     2641 ataatattac tgaagataaa gttataggct ctgaagaaag taatataatt gaattgattg
     2701 aagtagatga tagttttaac actgaagtag atagtaattt ggattataat gattttgaag
     2761 aaattaacag agaaattaga ataaataaaa aaataacttt actacttatg gaagcaattg
     2821 aagtattaat aaaaagatta agcatagaag atgaagatgt aataagatat attaaaaaat
     2881 atgaaaagat atttattgaa tctgtgctaa gctcaataaa aagtaacact tttaaaagta
     2941 tggtaactgt gagtaaaaat aaaaatgaac acttagaaga ctatattgtt gatgatgaaa
     3001 gtaaattgga tgatgaatcg tttagagaga tattcaatga gatattagat agtacatcta
     3061 taattgaaaa aataagaata ataaaggaaa atatcaatgc taaaaaagac tttatcgata
     3121 ttcttgaatc tgaatgttta tttggagaag agtatttaat gttgtttgca tcattaagtg
     3181 aattagaatt ggcaatattg ggtagtgtag tattttatga agatattaga atgagagaaa
     3241 ttaatatatt agaatttatg ttaaatgaga aaactgaaac tactttatgg aagacagagt
     3301 acattaaatt tatgaagagg caaagtgaag ataagatata ctccatagaa gagcatatga
     3361 ataaaattaa ttaacaaaaa attgtgaata aatagaatgt tagtatataa tataatctgt
     3421 aaagcatatc aaaaatacat atatttggag gaaaattatg aaagagtata tcataaataa
     3481 tgttgaagaa ataagagaag aattaattga tttatctaag aaaatatggg aaaatccaga
     3541 attagccttt gaagaaaagt atgcaagtag cattcaaaaa gaatacctaa agtcaaaagg
     3601 atttaaaatt gaagaagtag aaaatttacc tactggattt atagcaagtt ttggagaggg
     3661 taaacctgtg attggcatac ttggagaata tgatgcttta ccagagctat cacaatgtgt
     3721 gagtgcagaa agaaagccat tggtagaagg aaaagctgga catggatgtg ggcataacct
     3781 gttgggtgta gcaggtgtag gagcagttgt ttctataaaa aaattaatag aagaaaataa
     3841 atttaatgga acaataaaat attttggttg tcctgctgaa gaagaaggtg gaggaaagac
     3901 agttatgtgt ataaatggct gttttgatga tgtagattgt gcatttacat ggcatccttt
     3961 tgatataaat gcaccttgga gaggtggaag tctggcaaac ttatctgtaa aatttaaatt
     4021 taaagggata acagcccatg cagctcaagc tcctcataat ggtagaagcg cacttgatgc
     4081 tgttgaaatt atgaatgttg gagctaatta tttgagagaa catgttattg actctataag
     4141 aatgcactat gttataacta atggtggtgg aagacctaat gtagttcctg gatttgctga
     4201 aagttggtac tttattagag gtaaaaaagc aagggatgca gaacatgttc ttgatagatt
     4261 aataaaagta gctcaaggag cagcaatgat gacagaaact gaaatggagt ataaggttac
     4321 agatggaata tatgactata taccaaacca atgtttgact gatttagttt atgacaatat
     4381 ggtttttgtt ggttgcccta aaaatactcc tgaagaagaa gaatttgcaa aaaaattgtg
     4441 tgatactctt actagagaag aaagattagg tgtagcaaca tcattacaaa atgataaatc
     4501 tattgtagaa agttatatac acaaggaaat agttgacggt gacaaagata aaggactagc
     4561 tggttctact gatgttggag atgtaagtta tgtaatacca gtggctcaat ttgcaatggc
     4621 agcttggcct gttggaattg caagtcatac atggcagtca tgttcatcag caggttcaaa
     4681 tataggattt tctgctatga taaattcagc taaagtactg gcttgtagtg cttatgatgt
     4741 atttatgaat actaaaatta ttgatgaagc aaaaattgaa tttgataaat cattagatgg
     4801 acaaaaattt aaaccattgg tataattttg gtaataaaat atttctgggg ggaatagcta
     4861 tgagagaaaa agttgaaaaa gtgcttgagg aaaagataaa accagtatta caaagagatg
     4921 gtggagatgt agagcttata gatgtaaatg aaaatggtgt ggttttagtt agactacaag
     4981 gggcatgtag tgggtgtcca ggagcgacta tgacaatcaa agctattatt gaaaatgttt
     5041 tggtgagtga agttccagga gtaactcaag tattaggtgt ttaatagtta gcgtttatta
     5101 aatgtatgtt atacataagt atttaataaa gaagtaaaaa aaatagtgaa gcaatatata
     5161 tgaaaaagta tgataaagat aataaaacat tatagaaata atgaaatatt tcatatgtat
     5221 atgaagaaat aagaaaaaat agtgaagcaa tatatatatg aaaaagtatg ataaagataa
     5281 taaaacattt atagaaataa tggaatattt catatataca tatgaaaaaa tagtaaaaca
     5341 atatatatat gaaaaagtgt gagaaaataa taaaatacta tagaaacaat taaatattct
     5401 atataggtat taagaaacat gaaagaaata atggagtatt atagataaat atgtaaaagt
     5461 gtgaaagaag gatgtagata aatttgtctt atacaaattt acctacagta tttcttacac
     5521 actttttatt ttactatcca accataccaa taaacatacc tataaagtat ggaactaacc
     5581 atatagtcca tacaactata ctaaacttat gaaagaattt tttcttttcc tcatcatctt
     5641 tgtataggac aaaagtagcc caacttgcat gaaaaagcat aagcacaatt gcaagaagac
     5701 ctgtaatact atgaaggttt tgtgataaag cagattttac ttcaaaagta tggctgttgg
     5761 ctatgttact cattgtaaat gttcctaaag tatcaaatat aaacccaagc caaaatataa
     5821 ttacatgttt cttttttaaa atttttgcct ttctttctcc aaatacacct atagtgtaga
     5881 aaattaaagc tgatgttata aatactattg caagtattaa tttataattc ataattatct
     5941 ctcctttact tttgccaata tatttctaag attttttact aagtcactca attcgttttc
     6001 atcacaattt ttaaaaatat catcaaaact ttcttgcatt ttaattttaa aatttgtggc
     6061 aaattcaaat cctgttgttg taaacttaac gtatgtattt cttttatcat tagatttctt
     6121 gaatttttct atatatccaa tctgttctaa tctgctgatt atgcctgaaa ccgtaccttt
     6181 agctaaagac atttcatcac atagttgtga tattgtaagt tcttgattat gagctaccag
     6241 ttttataact ataatctgtt ggtgtgttaa tccattttct ttaaaatttt cttctactgt
     6301 gtacatagtt ttagaataaa gttcttttaa gagcatggct attctaaaac agtcataatt
     6361 acttttcaat aaaatcacct cttaaatggt ttgcatacaa actaatataa tatacttttc
     6421 ttatatagtc aacctaaaat tttataattt tatagaaaat aatgaagaat agaacataaa
     6481 aattttattt taggtataaa taaaataatt tgatgaaatt ttaacaattt ttaaaaagtt
     6541 tgtttacaca gttaataaat gatgctaaaa ttaactcata gataaatata gaaaatttaa
     6601 ttttgtttaa tgataatgga accacgaagg ttttaacgtt actttacgtt tgcttttatg
     6661 tggttttttt attgcaacaa aggggggttg gggtttgtta aaggatatac tgaaaaagtt
     6721 gttgcagttt attttagtaa tgtttttatt atcttttatt gttttttaca tggcaagact
     6781 tgctccagga gacccattaa tctcttacta tggggatgga gtagaaagaa tgagtacaca
     6841 agaaaaagaa aatgctatga aaaaacttgg tttgaatgaa ccaatataca gtcaatatat
     6901 aaaatggatt tcaaatgcat caaaagggga atttggaatt tcatttaaat ataaacaaaa
     6961 tgttacttct gttataaatg atgtctatat aaacactata atattaggtg gttcagggta
     7021 tatacttacc tttgtgctag ccttattgtt aggaatattt tgtacattac atgaagataa
     7081 gctcattgat agaatcatat gcaaattagg aacaataacc aactgtattc catcattttg
     7141 ggttgcctta gtactcatac ttatatttag tataaactta agtatacttc caagtagtgg
     7201 tgcttattca atgggagaag agtccagtat attaagtagg atatcacatc taatcttacc
     7261 tcttacagta cttatattaa gccatctatg gtattacaca tatatgataa gaaataaatt
     7321 actagaggaa ataagagaag attatgtgct tttatgtaaa gcaaaaggtt taaataatag
     7381 aactattgtt tttaagcatt gcctgagaaa tataatgcca tcatatatta gtataatggc
     7441 catttcgata cctcatatac taggaggaac ttatgtagtt gaaaaagttt tttcttatcc
     7501 tgggttagga acactttgtt ttgaaagtgc aaagtatcat gattataata tgttattagt
     7561 tttatgctta ataacagggg ctttagtagt atttgggaat atgttagctc aaattataaa
     7621 caataaaata gaccctagaa tgaaatatga tagaggtgat actaatgaag ccacaatata
     7681 gtgattttga aattgtagga gaaaattata ttttagtagc agaagatgaa catatagaaa
     7741 caaagaaaac cttatctaaa aaaattaaag aattaccata tatatcaatt attattttat
     7801 caatcatagt cattggaagt gtattctcat cttttataat gacacatgaa ccaacataca
     7861 tggatttagc aagttctaat ttagctccaa ataaaacttt tttatttgga acagactcaa
     7921 tgggtagaga tatatattct atgatatggt atggaggaaa aatatcttta tttataggac
     7981 tattttccac tgtaatatca actgttatag gaattgttta tggtagtata agtggttcag
     8041 catctgaact cgtagatgat gcaatgatga ggtttacaga aatcatactt agtatacctt
     8101 caatattaat tataatattt gtacaagcta tacttggaaa ttcaaatcca gtatctatgt
     8161 ccatagtaat agggataaca agttggatga atatatctaa gatagtgaga acagaagtta
     8221 gacaaataag aaatagcgaa tacatactag cagctaagag catgggagga ggattcttct
     8281 atgtattaaa gcaacattta cttccaaatt ttgtggcatc aataatgttc atggttgtaa
     8341 caaacatagg cgcagctatt ggtactgaat cgacacttag ctttttagga attggtttac
     8401 caatagaaat tgtttcttgg ggaagtatgt tatctttgtc agaggaagca ttactttcaa
     8461 atagatggtg gattatactg attccgggta tatttttggt taccacattg gtgtgtataa
     8521 caaatatagg taattatata agaaagaata ataataaaaa atctagtaat ttataaatca
     8581 gcatttgagt actaaataag caaatactga ggtatttgaa atatctattt aaataaatgg
     8641 ggtcaaagct ttgaaaaata ttttataaaa agcaaaaggg ggagcatcat gaaattaaag
     8701 aagttaaaag ttttaagttt agtaatgata cttagtctaa tggcaggttg ttctagtgga
     8761 ggagacaaag ataagaaggc agatacgcct aaagatggaa aggtacttgt ttatggaagt
     8821 aatgactata caagtataaa tcctgcacta tatgaacatg gagaaataaa ttcattaata
     8881 tttaatggat taacagctca tgatgaaaat aataaggtgg ttccttgtct agcaaaggac
     8941 tggaaatttg atgaggcaac aaatacatat acttttaatt taagagatga tgttaagtgg
     9001 catgatggag agaaatttac ggcaaatgat gttaagttta caatagaaac tataatgaat
     9061 ccagataatg cttctgagat agcatcaaat tatgaagata taacaaaaat tgatgtggtt
     9121 aatgataata ctataaaaat tactttaaaa gctccaaata cagcaatgct tgattattta
     9181 acagtaggag tattaccaaa acatgttttg gaaggtaagg atatagctac tgatgaattt
     9241 aatcaaaaac caataggtac tggaccattt aaacttgaaa aatgggacaa aggtcaaagt
     9301 ataacacttg ttaagaatag tgattatttt gtaaaagaac caggattaga taaagtagtc
     9361 tttaagattg ttccagatga taaggctaag gcaatgcaat taaaatcagg agaattagac
     9421 ttagctcaaa taactcctaa agatatgtct aactttgaaa aagacgaaaa gaattttaaa
     9481 gtaaatataa tgaaaacagc cgactataga ggtatattat acaattttaa ttctaaattc
     9541 tttaaagata aaaaagctaa aggattacca aatgctttaa gctatgcaat agatagaaaa
     9601 gcaatagttg acagtgtatt attaggacat ggtgtaccag catattcacc tttacaaatg
     9661 ggaccatata ataatcctga tatagaaaag tttgaatata atccagaaaa agctaaacaa
     9721 gaaattgaaa agctaggttg gaaattaggt tctgatggaa tctatgaaaa agaaggtact
     9781 aagttagctt tcgagataac agcaggtgag agtgaccaag ttagagttga tatggctaaa
     9841 atatgtgcac aacagttaaa agaaattggt gttgatgcta aagctgttgt tgtaactgaa
     9901 acagattggg ctaatcaaga tgctcattta ataggttggg gaagtccatt tgacccagat
     9961 gaccatacat ataaagtatt tggaacagat aaaggtgcaa attacagtgc ttactctaat
    10021 cctactatag ataaaatact tcaaaaagca agagaaacag aagataaaga tgaaaaatta
    10081 aagttatata aacaattcca agtagaaatg actaaggata tgccttatac atttattgct
    10141 tatatagatg caatatatgt tggaaaacca aacataaaag gattaacacc tgatacagtt
    10201 ttaggtcatc atggtgtagg tatattctgg aacatagctg actggacaat tgaataatac
    10261 tgaaattaat tagaaacctt agggataaaa atatatccct aaggtttgaa tagcataggg
    10321 gttgatggtt tttggaacat ttattggagg ttaataatct atctgtaagt tttaaagtag
    10381 aagaaggaga agttcaggct gtaagaaatg tatcttttaa tctaaaaaaa ggtgagacat
    10441 tggctatagt tggagaatct ggttgtggga aatctgtact gtgtaagagt ttgatgagga
    10501 tactcccata caatggatat attaaaaatg gagaggtttt acttaaatca agtgatttgg
    10561 ttaagaagtc tgaaaaagag atggaagata taagaggaaa gaatatatca atgatatttc
    10621 aagaccctat gacatctctc aatccaacta tatcaatagg aaagcaaatt gcagaagctg
    10681 taatcattca tcaaggtata agtaaaagtg aagcaaaaaa gagagccata gagcttattg
    10741 aattggttgg aattgataat cctgagaaaa gatttaaaca atttccacat catttttcag
    10801 gtggaatgag acaacgtata gtaatagcta tagcacttgc ctgtaatcca gatgtattga
    10861 ttgctgatga acctacaact gctttagatg taactataca agctcaaata attgatttaa
    10921 taaaggattt acaacataaa ataggtttgt caataatatt tataactcat gatttaggag
    10981 tagttgcaac tatagctgat agaatagcag ttatgtatgc aggaaagatt gtagaaatag
    11041 gaactgtaga ggatatattt tatgacccta gacatccata tacttggggg ttgttgggtt
    11101 cattacctac tttagattca caggatgaat atttatataa tatacctgga atgcctccaa
    11161 atttattgaa cccaccaaaa ggagatgctt ttgcaataag aaataaaaat gcacttaaga
    11221 tagattatga aaaagagcca ccaatgttta aaattaatga tacacatagt gcagcaactt
    11281 ggttattgca cccagatgca cctgaagtag atgtaccagt tagagtaaat tgtggaaggg
    11341 tgatttctaa tgaatagaga aaaaatactg gaaataaaaa acttaaagca atattttcat
    11401 ttggacaaaa gtactactgt aaaagcagtt gatgatataa gttttgatat atataagggt
    11461 gagatatttg gattggtagg agagtcgggt tcaggaaaat ctacaactgg taaaactata
    11521 ataagactac atgaatctac tggtggagaa gtaatttaca aaggaaattg catatcagat
    11581 aaaaaaacgt acaaatttat aaaaaaagat gtaaacaaaa gtatgcagat tatttttcaa
    11641 gattctactt catcattaaa tccacgtatg acaattgcag atataatttc agagccacta
    11701 aaaattcaag gtatctgtaa aaataaaaca gatagaatga ataaagtata tgagatgcta
    11761 aagctagtag gacttgatag aagttatgca aataagtatc catctgattt ttcaggtgga
    11821 caaagacagc gtattggcat agcacgagct ctttcagtag acccagaatt tataatagct
    11881 gatgagccta tagcttcttt ggatgtgtct atacaagcac aaatagtaaa tttatttaaa
    11941 aaattgcaac aagaaaagaa tttaacttgt ttatttatag ctcatgattt atctatggtg
    12001 agacatatta gtgaccgtat aggagttatg tataatggaa aattagtaga attggcagat
    12061 tcaaatgaat tgtataataa tcctattcat ccatatacaa aatcactact ttctgcaatt
    12121 cctgttcctg accctagata tgctaagtca agaaatagaa tagagtataa ttctaatgga
    12181 tatgattgtt caaatgaaaa gtcattaagt tggattgagg tttcagatgg acattttgta
    12241 tattcttcta aatcagaaat aaataaatat caacaaaatt taaaagttgt ttagtataaa
    12301 aatacttgaa caggtattta tttgaaatac aaactaaaac cctatttttg gagaaatttt
    12361 aaaactactc caaaataggg ttttttattt ctacttttca aatataataa attcaaatat
    12421 aataaaatta aaaacaagta aagtatgttg ttaagaaagt gctataaaat taaaaatagt
    12481 aatgtagaaa aaatgagcaa taatcttatt ttttctacaa taattttcta taatttaaag
    12541 taaggaaagt ttgataaatt ataaccttag ttaaagaata agagtttatt aaaaaataaa
    12601 gtgtgtatag gggttttagt taataaaata ttataatgta ggggagcttg taatgatata
    12661 taaaaaagat aaagtaatga aagttttaga atcatcatat agggaaaatg aaaaagggat
    12721 tacagctaac caattatcag aaaagacagg aattgcaaga aacaatataa gtacatattt
    12781 aaaccaatta ttcaatgagg gtatggttac gaagataaaa ggaagacctg tgtattatat
    12841 ccctgcaaat cttaacctta acaagaatga attgcttgat acacaaagtg agttgcttgg
    12901 cataaaaaaa ggaacaaaag tagaaaacaa aataaaaaaa gatagttttg aaacactaat
    12961 aggtaaagaa catagtttaa gacctataat agaaaaatgc aaggtcgcaa tgctctatcc
    13021 tacaaatgga cttcatacta ttatctatgg agaaacagga gtaggaaaga gtatgattgc
    13081 tagatatatg tacgattatt ctatagatag tggaattaga aaaaataaag ctccttttgt
    13141 aacatttaat tgtgctgatt atgctaataa tgcacaatta cttatgggac atatttttgg
    13201 agtagaacaa ggagcttata caggtgcaga atcttccaga aaaggtcttt tggaaattgc
    13261 aaatggaggg atactatttt tagatgaaat tcatagacta ccagcagaag gtcaagaaat
    13321 gttgtttact tttatagata agggaacatt taaaagacta ggagatgcaa cgaaagaaag
    13381 agattctaag gtattaataa tatgtgctac tacagaaaat ccgacatcaa cactattaga
    13441 tacattcaat agaagaatac caatgaaaat agaaatacca tcactgagag ataggacaat
    13501 gattgaaaga atggaattag tcaaaaattt tttcaaggaa gaatcaaaaa gtatttctat
    13561 ttcaataaaa gtacataaag aaataattaa atcactatta ctttatgatt gtaaaaataa
    13621 tgtaggtcaa cttcaaaatg atataaaatt agctgttgca aatggatacc taagatataa
    13681 aagagataat aaagaaccag tttatataga gaaaaaatat tttgataaat ctataaatga
    13741 agtgagtgat gattatagag agaagtctat taaagcagat gaagttataa cacatggaat
    13801 agaatatttt gtatttacgt cattaggaga agaagaagtt attaactaca atgatataaa
    13861 tattattaat gcagtaaata ctaatcttaa taaatctgta gaaatgaata tagcagatgc
    13921 ttctctattt aataatgtta agtttaaaag catgtgtgaa tcaataagac aaattattaa
    13981 agaagatgaa gaaatagata taagtgaatc aatatttaag tcaatggcaa tttatataaa
    14041 aagcgtattg gataaaagta gctctacaaa caacatagat ttgaatcaaa ttagaagaaa
    14101 taacagaagt gaatttaaaa cagcactaaa aatagttgga ataattgaaa atgaatttga
    14161 tttattttta tctatagaaa ctgtagcata tattacatta ttcatagtac aagctaaaca
    14221 agatgaacaa aataaaatag tgaataacat taatattata gttgctatgc atggagaaac
    14281 tacagcaagt aatggtaaaa gcagttggtg atattattgg aaagtgcagt gctatttctt
    14341 tcgatatgaa gttagataaa agctatgatg aagttattgt agattttaaa aatttaatta
    14401 ataatataga caataaagat gtattactat ttacagatat gggttcatta aactcctttg
    14461 atgaaattat aaagaaagaa aaaaagtgtg aagtaagagt aattcctatg gtgacgacat
    14521 taactgtgtt agaagcagtt cagaaggcca atatgggatt acctttaaat gacgtataca
    14581 actctatcac taacacgaga aagtattatt ttggaactaa tgaaattcaa aataaagaaa
    14641 atctgtctaa aacaattatt atagcatcac atgtttcaga aggtgtagat aataaaacaa
    14701 gaaaaatatt agaagaaaag atgagtaggt acttagatgg aatagatatt atatctgtcc
    14761 catataaaac agaaaaagat ttaagtttaa atattacaaa acttaaagaa agtagtaata
    14821 ttgttgcagt aatcaatgaa caaagaatta atattagagg aattgactat attagcaaaa
    14881 aagatataga taaggatgaa aatatcaata aattaaaaaa tattataaag ataagtatag
    14941 gttatgatga tgtagttgaa ggtttaaaga catccttaaa aagctctaat tataatagga
    15001 tatttaaaga tataaaatat gtgtctgatg aattgttttt agtatttaat atagaaaaga
    15061 aatacgataa agtaattgga cttatgatgc atttagcttt catggtagat gggttgattg
    15121 gaaatactag agaaattgaa aagcttgata aagaaaaaac acttgattat cacaagagcc
    15181 taagcaaaat aaaagatata gtatctcaat tagataaaaa atataatata gaaattaatg
    15241 aaaaagagtg ctatcaaata ttattaatat tagaatatgc agaaataatc gaaaaagatt
    15301 accaatgagt ataaaaactt atttggtaat tttttttagt ctaaaataat attaatatta
    15361 gacacatata aatatactta gacaaagtat ctaagttttg atgataaaaa tgattgaaat
    15421 aacagtgttt taaagtgtct aaaaaaactg gcataatact tgcttatatt attaaatgag
    15481 taatataaat aattaaaatt taaataaaaa ggagtaaata atatgcaaga taaaatatta
    15541 agagataaaa aggcaaatga cccatgggca agaatgacta caagaaacgg atttagtgca
    15601 gacgaaataa tatcgtcatt tcaaaaagct ataagaagaa atatggtgga agaagcttgt
    15661 gaatttgctt atgaactata tatatcatca ccagaattag aagataagct atggagaagg
    15721 atacttacaa tatcagttga agatatagga atgggggacc catcagctgt aataatcata
    15781 aataatttaa atcaagtaag aaaggaatat tcatatgcag atggagacag acctttattt
    15841 ttcatacatg caataagata tctttgtgca tgtgagaaag atagatcaag tgatttatta
    15901 aaaaatataa ttataaaagg atttgcaatg ggtaaagttc cagaagttat ggacgtggca
    15961 cttgacaagc atacatatag aggattagaa atgggaaggg attcattcca ctttttaaat
    16021 gaagctagta tagtaatacc agaaaaagaa gtagataatg attataaaga aagatactta
    16081 aaaatattag aaaaatataa gcaagaagaa gttatagata cggctttcaa gtttaatcat
    16141 tggcagtact aagaattaga gtaaatatta ataagaatta aaataaaata ataagaatta
    16201 aagtaaaaac tgataggaat tagagtaaga attggctgat aaaaaattag aaaataaaag
    16261 aggttagata ttgtaggagg gtaattatga atattttatt agtatgtgca aatggagctt
    16321 caacaggtgt acttgtggaa aagatgaaaa gtttttgtag tgagcatgaa aagttaaaaa
    16381 ctaaaactat aaatatagag gcaacatcat tcgaaaattt aaaatcatac attgaagcta
    16441 atgatacaga tgtagtactt gtagcaccac aaattaggtt caaggaagat gatgtagttg
    16501 aagcttgtaa gaattacaaa atagcagttg gtttaataga tactaagcat tatggtcgta
    16561 tggatgctcc ttctgttatg aagtcggcaa tcgaattata taaaaataga taattggggg
    16621 gaataaaaat ggaagcactt caaaataatt taagaagatt tcttctacca atagcacaaa
    16681 agattgaaaa acaaaggcat cttcaagcta taaaagaagg tatgataagc attaccccaa
    16741 taataattgt tggttcttta agtatgttat ttatggcatt aaacaatatg ttacctgaag
    16801 gaagtgcaaa aacattatta tctgaaaata tggatacatt acttatccct aataaattta
    16861 ctatgagttt attatctata tattcagcat tttttatagc tcaggcacta gctaaaaaat
    16921 ataatcttaa tcatgtagaa ataggtatga cagcagttgt tgcacaactt gttgtatgtg
    16981 gacaagtagt agatggtgta ttagatacaa gttatttaga tgcacaaggt ttatttgtaa
    17041 gtatattagt agcactttta gtagttgata ttactaagtt tatgaatgat aaaaatctcg
    17101 taattagatt tccaaaagaa gtacctagtg tagtaaataa aagttttaga aatttaactc
    17161 caatgatagt atgtataatg ttatttacag caatagcagc aataactaaa aatgtctcag
    17221 gtcaaccatt accagctatt ataatgaatt tcttagcacc agctataagc agtgtagata
    17281 atgtttttgc agtaactata atactattta taactcaatt attatggttc tttggccttc
    17341 atggagcagc aataacttca agtatatgga tgccaatagc agctacatat atggcagaaa
    17401 atgcaacact gatagcagca ggaggagacc caaaatatgt gtttacaata ggattttact
    17461 atggtttctt gcaagtaact ggttcaggga taactttggg attagtgtac cttatgtcaa
    17521 gaagtaagtg taagagtttt aatagtatgg gaaaagttgt gattttacca tcactatttg
    17581 gaataaatga gccagtaata tttggaacac caatagttat gaacccatat atgtttgtac
    17641 catttgtatt tggacctgta ttggttggag cacttaattt tatggcatta aaagtaggat
    17701 tagtaggatt acctattgca gaaccaccag gctttttacc tccaggggtt gcagcatttt
    17761 tgatgacatt agattggaag gcaatcgtat tagtatttgc aagtataata cttatgacct
    17821 tgatatatta tccattcttt aagataatgg agaaggaaga attaaataaa aatgcagaat
    17881 tagctacaac attagatgat gatagctttg atttttaaca agtcattaga aggagaagga
    17941 tatggtacct gaaatggaag aaaaagtatt tagtataata tcagaagcag gagatgctaa
    18001 aagtgatgta atgatgtctc ttaaggaaat aaaaaaaggt gactacaata aagcaaaaca
    18061 ccttttaaat tcagcctcag agaaaataca aacagcatca aagcatcatt tagaattact
    18121 atcaaatgct atgaatagtg aagatagtgg gactgatttt ttagttgtgc actctgaaga
    18181 ccattattca aatgcattat ttgctcattc tttagtatct gaacttgtag atatatttga
    18241 gatgatggac ccaagaatta ataagaatta aatttcttgt aaatagtgct tgaagtttgg
    18301 ctatagttta ttaaatcaat agatttaata aatgaactcc cttttacttt aaagtactag
    18361 tatttataaa tttatttata aatacagtaa atttttaaat agaaaaggag atattattat
    18421 ggaaaaattt tcagtagtaa tagctggagg aggaagtaca tataccccag aaatcatatt
    18481 aatgttgcta gataacttag ataggttacc attaagagca atcaaattat atgataatga
    18541 tgaagaaaga caaaataagg tagctaaagc ttgtgaaata ctaataaagg aaaaggaccc
    18601 taatatagag tatttagcaa caacatgccc taaagaggct tatacagatg tagatttttg
    18661 tttagctcat ataagagttg gaaaacttga aatgagagaa ctagatgaaa agatacctct
    18721 aaaacatgga gtagttggtc aagaaacttg tggaccaggt ggaatagctt atggaatgag
    18781 gtctatagaa ggtgttcttg aaaacataga ttatatggaa aagtattctc caaattgttg
    18841 gatgttaaat tattctaatc cagcatcaat aatagcggaa gctgttagaa ggttgagacc
    18901 taattctagg gtaataaata tttgtgatat gccaataggt atggaacata atatagcaag
    18961 gattgcagga ctcaaatctc gtaaacatat ggatataaga tactttggat taaatcactt
    19021 tggttggtat acttcaatta aagataaaaa aggaaatgaa cttttaccaa aattagtaga
    19081 acatataaaa gaatatggtt ttataaatgg tgaagaagga atgaaaaatg acaagaaaga
    19141 tagttggttt gaaactaatt tatttactaa agaaatagta aaaacagatt taactacaat
    19201 accaagtagt tacttaaagt actatctatt tccagattat gttgtaaatc attctgatgt
    19261 aaattatact cgtgccaatg aagttattga tggaagagaa aaagaagttt ttggttcatg
    19321 tgcattgata gaaaagcaag gacattctaa gggcagtggg cttaagatag gaatacatgc
    19381 agagtttata gtggatttag ctacagctct agcttttaat acacaggagc gtatgttatt
    19441 gatagttcaa aataatggag ctatagaaaa tatacaagat gatgctatgg ttgaaattcc
    19501 atgtatagtt ggaaaagacg ggtatgaacc attatcaata gggaaaatac caacattcca
    19561 aaaaggttta atagaacaac aattagcagt tgaaaaatta gtagttgatg cttggattga
    19621 gaagtcttat aataaattgt ggcaagcact tatgctatct aagacagttc ctagtgcaac
    19681 agttgcaaaa gaattactag atgatttaat agaagcaaat aaaaattatt ggccaaaact
    19741 taattaagaa taatatattt ttaaagttcc tacagtttag tacatagcct agttaatgta
    19801 tttgtaggga ctttatttat ttacaaatga gatacaagtg gcaaacatag tgtattttgc
    19861 agttaggaaa gtaggtttag ttacttgtta gttggattaa atttaggtac aataatttac
    19921 tatcaatcaa tagaaataac cagagaagtt gcataataaa aaatttacac ttttaacagg
    19981 atttatgtag gtgtttatag aaataaataa atatgtacat aaatacataa ttatttttaa
    20041 gtctaaaata caagaatgga gagatggcta tgaagtggag agattatgct gatgatgtaa
    20101 atttatttga ggattttgat aaaactatca agcctctaac tgacgaacaa agaagaaaaa
    20161 atataaacac tttaattgca tatttttcaa aagaagttcc ttctaaaata tacaatttat
    20221 caaatgatga aattaagcct agagatatac ttagaggact tttgaatgta tatccaccaa
    20281 aagaaattgc tccagagata cttaatatgt tacataattt attgttgatt gaatgtgaag
    20341 aaagagagtt agtagatgta aatgatatag aagaagtaga ggaagggatt gccatatgga
    20401 gaggtaatat aacgaattta agagcagatg caatagtaaa tgctgcaaat aacaagttat
    20461 tagggtgttt acaaccatta catctatgcg tggataatga gatacattca tgcgcaggtc
    20521 ctagacttag agaagattgt gacaaaataa taaaaaaaca agggcattta gaatatactg
    20581 gagatgctaa gataactaga ggctattgtt tacctgcaaa atttgtagta catactgttg
    20641 gacctattgt atctggtggt cagccaagta aagagcaaga aaagcaactg cttcattgtt
    20701 ataaatcatg tttaaataca ataaaagaaa ttgatgaaat aaaaaatatt gttttttgtg
    20761 gaatttctac aggtgtattt ggttatccaa aaaaagaggc agctaatctt gctgtaagta
    20821 gagttagact atggctaaaa gaaaatcctg aaaaaaattt aaaagttgta tttaatgtgt
    20881 ttactgaaga agaagaagag aaatatagaa gaatatttaa ataatattat gtaacagaat
    20941 cctaaaaata cttattatat aatacaaatt cattgtataa gataataatt tacaagtagt
    21001 aaaaattaat aaaatataat ttgtattttt aataaataag taggtataat agaaattata
    21061 agaatagtat aatcaattat gctattactt agaaaatata atataggaaa attgtatggt
    21121 aaataaatat gaaaaatagt tttaaaaatt gtattagttt aagaaataaa tttataatta
    21181 tatcaattgt gtttctatta atcctattaa ttgtatctta tatatttggc aaattagtgt
    21241 ataatagttc ggttggttta gagcagttaa taaaaaacga agatatagta aaaatatttt
    21301 ctgagagaaa agataaacca ctggagaaat taaaaaacta caagacatct gaattaatga
    21361 tacaaagttc taataattac aagcttgaaa gtctttttat aacttcaaat ataaagacaa
    21421 gagatactat gatacttgtt catggaattg gaagtagtta ctatgaaatg ttaaaggtag
    21481 catatagata tttagataaa ggttataatg tattagttta taatcaaaga aatactggta
    21541 atagtggtgg agataattac acatttggtc tttatgaaag gtatgattta gatagtctag
    21601 ttaaatttgt aaaaaataaa tttcctgaag gaagattagg agtgcatgga ttttccatgg
    21661 gagcagggac agctgctatg cattcagaga taaattcaaa agatgataag gtcgattttt
    21721 atatactgga ttctccttat tctgaaatga aagatgcaat tagaatggga gtactagaaa
    21781 aacgaatacc agatatatta atcaattatg tggtgatttg tggagattta tataataagt
    21841 ttaaatctgg attttggtac agtgatgtaa aaccatatga gtcagttgag aaatctaatg
    21901 tacccatact gtttatacat ggaacaaaag atactgtttg taattatcaa aatagcaaga
    21961 aaatgtatga tttagttaaa catgataaaa aagacctatg gcttatagaa ggtattggtc
    22021 atgttgatgg atttgaacat gatagtacag tatacttcaa taaaatattt aaatttatag
    22081 atagtaatgt tttaagtgat aaaagttaat tgaatattag tagcttattt atttttgtat
    22141 ttattaaaaa atagataggc tatttttatg tttaaatata agttttgttt agtaatgtga
    22201 attaatttct agtaaaaatg aaaataacta ccaattaaat tcatttatac ctcaaataaa
    22261 agagttagta ttagattaca atcaaatcta aagaaaagag aggtagtata tatga
//
LOCUS       DAEPLF010000043        22241 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEPLF010000043 DAEPLF010000000
VERSION     DAEPLF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.043"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(345..1106)
                     /locus_tag="KR314_001448"
     CDS             complement(345..1106)
                     /locus_tag="KR314_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0327839.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            1325..1801
                     /locus_tag="KR314_001449"
     CDS             1325..1801
                     /locus_tag="KR314_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBH0327840.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1899..2051
                     /locus_tag="KR314_001450"
     CDS             1899..2051
                     /locus_tag="KR314_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBH0327841.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(2211..2399)
                     /locus_tag="KR314_001451"
     CDS             complement(2211..2399)
                     /locus_tag="KR314_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBH0327842.1"
                     /translation="MAKVCSVCDKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            2760..3110
                     /locus_tag="KR314_001452"
     CDS             2760..3110
                     /locus_tag="KR314_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBH0327843.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            3129..4772
                     /locus_tag="KR314_001453"
     CDS             3129..4772
                     /locus_tag="KR314_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBH0327844.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            4853..6910
                     /gene="recG"
                     /locus_tag="KR314_001454"
     CDS             4853..6910
                     /gene="recG"
                     /locus_tag="KR314_001454"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBH0327845.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIRNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMERFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            6969..7523
                     /gene="rsmD"
                     /locus_tag="KR314_001455"
     CDS             6969..7523
                     /gene="rsmD"
                     /locus_tag="KR314_001455"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBH0327846.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNVKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            7525..8022
                     /gene="coaD"
                     /locus_tag="KR314_001456"
     CDS             7525..8022
                     /gene="coaD"
                     /locus_tag="KR314_001456"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBH0327847.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            8026..8550
                     /locus_tag="KR314_001457"
     CDS             8026..8550
                     /locus_tag="KR314_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327848.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            8894..10813
                     /locus_tag="KR314_001458"
     CDS             8894..10813
                     /locus_tag="KR314_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0327849.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQERFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISKEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            10826..11113
                     /locus_tag="KR314_001459"
     CDS             10826..11113
                     /locus_tag="KR314_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0327850.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            11246..12505
                     /locus_tag="KR314_001460"
     CDS             11246..12505
                     /locus_tag="KR314_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBH0327851.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISKFIGSLFGGIL"
     gene            12508..13248
                     /locus_tag="KR314_001461"
     CDS             12508..13248
                     /locus_tag="KR314_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0327852.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            13571..14881
                     /locus_tag="KR314_001462"
     CDS             13571..14881
                     /locus_tag="KR314_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBH0327853.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            14979..15896
                     /locus_tag="KR314_001463"
     CDS             14979..15896
                     /locus_tag="KR314_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBH0327854.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            15912..16721
                     /locus_tag="KR314_001464"
     CDS             15912..16721
                     /locus_tag="KR314_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBH0327855.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            16778..18526
                     /locus_tag="KR314_001465"
     CDS             16778..18526
                     /locus_tag="KR314_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBH0327856.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKEKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSTISWKGLENRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLNIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDKSKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMSKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDENDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIENLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            18789..19811
                     /locus_tag="KR314_001466"
     CDS             18789..19811
                     /locus_tag="KR314_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBH0327857.1"
                     /translation="MFGGINIKRIGIIIMFFLSIAFIGFYLAIRGNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEEFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNVPKDSNIVV
                     SFHYYEPNDFAFQGNIYHNGFEHLSNITWEGTNEQMDYLKKRFDIVENWANKNKVKIF
                     LGEFGVTKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            19816..22122
                     /locus_tag="KR314_001467"
     CDS             19816..22122
                     /locus_tag="KR314_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0327858.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPVISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKNKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKIRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKNIYPKEIALEINENIVLECDLCEGKIDERGLLYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTIPVSNPSEKKEKSIDEFEPMEYI"
BASE COUNT         8723 a         2369 c         3961 g         7188 t
ORIGIN      
        1 aattcatgac aatgatatta gagatagtat atctaattgt aatgattttg ataattttct
       61 ttccataatc aagtctatag tatagatttt tatattacat aagttttaaa aactttaata
      121 aaatattttt tgtttttagc ataaatagta taagtaagtt ttgtataatt tgttattatt
      181 ttgtattgat tataattatt atacaataaa gattctgtct caaaatagag agagaaactt
      241 gtttttcatt gataaaatga taaaaatata tttgatttca tacaaaaaag agtgtctcat
      301 gaactaaaaa gttcattttg agacaccacc tttattattt tatattattt atctcttgta
      361 tatattacta tagcatccct taaaaactta gctaatccag gttgttctct atcataatat
      421 gatgtaaaac gttcatcata tacatacatc tgtgctagag aggaatgagc ttctttagag
      481 tatgtgttcc aagtgtaact caaccattta tgatgtaatt ttgcaacttc ctgagccagt
      541 tcactagagg catcaccagt tttaaaagct tttttaagtt tactaattat ttcgatattt
      601 aggttttgcc aattttcata atcttgctta gacatatttt taagcttttt atttgatgta
      661 tctataatat ctttaccata tttctctcta atttcagttc cataattttt ttcattttca
      721 tcaatcatct tttctttaaa accttcaaat ttttctatat ctgtcatagt gtattttccc
      781 tccttcagag ctatagtttt agttacattt tctattagta aatcaatttg ctttcttttg
      841 gctagtaatt tattatggtg ttcttttaac gcacttaaac tatcgaagtt tggtgaattt
      901 ataatgtttt ttatattttc taagttaatt cctaattctc tataaaaaag tatttgttga
      961 agtttattaa cttcattttg accatatata cgataacctg aagaatttat tttaagtggc
     1021 ttaagaagac ctatttcatc ataatatcta agtgttctag tacttatacc agctagttta
     1081 cttaattttt gaactgtata ttccataagt aatacctcct ataaattaaa tatacacttt
     1141 tacgcaacgt aaatgtcaat atattaaaat ataaaaattt atatctttta ataaaaatat
     1201 aaaaaaatat ctttactgga tattttacac acaactgtca atactcacta atgaaaatta
     1261 aaaaataaaa tttatagact atctttacta aaatatagaa ggtctatagc ctaggaggaa
     1321 ctgtatgaat ttaaaaagaa gcaatgaaaa acgagaagag tacagaagaa tgtattcaga
     1381 taaagaaagt tttttaagtt tgattcaaaa ttttacaagt gtctcaatag caaaagaatt
     1441 aactcttaaa aatatagagt tagaaacatc ttttatatgt gaatataaag gaaaagaagt
     1501 tgatataatt tataaggtat tttctaaaag tggcaaagta tcacattata tagtgttaga
     1561 atttcaaaca gaaatggata ctgaaattgt cccaagattg aaatcgtata gagaacaaat
     1621 ttggaaaagt tttattatga aaaaaagcct tgaagaaata gaagataaaa attttaaact
     1681 tcctaaagtt ataccagtgg ttttatatag tggacctgaa aggttaagta gaaaaaaagg
     1741 aattttggat attgtagaat ctatatcgga gttcagtaat aattctagta tgaaaaatta
     1801 aaattacata atgtgtttat tggtctaaaa tggaaaactg tcgcataaac ttgaatatga
     1861 aaatatttat agatacaggt tctatgtgga ggtgttccat gagaaataac aagtctaagg
     1921 tattgctagg aatactttgt ctagcaacag gaatggctgt aattctgtca atgttgcttc
     1981 ctggctggat ttggtcagca cttaccgcat taatacttat tggatgtgga gcattattat
     2041 ttttttgtta aaatagtttt aactataaag tgtggggtga gtattgatga aagtagtaac
     2101 aataaaatta ccaaaatgga tttcaagtat aatcctaaaa tttatgagaa agagtaaaaa
     2161 tgattaaatt aaaaagccta cttacggaag taggctttta aacgtggtta ctaagctctt
     2221 tcaattttac cagaacgtaa acatctagta caaactttta ctctcttagg agctccatct
     2281 ataatcgctc ttacgcttct taagttagct gaccatgttc ttttattatg tttatttgag
     2341 tgtgatactt ggtttccaga aaccttacct ttatcacata cgctacatac ttttgccatc
     2401 tacgcacacc tccttaaaat gataactatt taaagactaa aaacatattt atattatcat
     2461 aaataacaaa aatttgcaat agaaatatat atttgcctta cacatattga agaatttctt
     2521 attatcatat aaaattatat atataatttt atataaagta aatatatttg gaaaataatt
     2581 attatttttc tttgaatata tatactatag aagaaatttt atccaaaaac ttttctaggt
     2641 ttaaattata ccatatttat agtatgatat gattataatt ttatataata aataagtaaa
     2701 aatagtaaaa aataaattaa gaaatgtttt taataaaaat aagcttaagg gggtcaacca
     2761 tgagtgcaaa ggtaacgaat caatatggaa atatagaaat agataatcaa gtaatagctc
     2821 aagtcgtata tagagctgca atggaaagtt atggcttagt aggtttagca tataagtcta
     2881 aaggaatagt agagctatta aaaggcgaga acgcaactaa aggtgtaaga gttgaggaag
     2941 tagaagaaga tgccatagca atcgaacttt atgttataat acaatatggt acaaatatat
     3001 ctactgttgc caataatata atagatagag taaagtatgt agttgaaaaa atgactggcg
     3061 taagagttac tagaattgat attaatgtac aaggaataag agttaagtaa tttcatagga
     3121 ggaaacaaat gattcaatat atagatggta aaaggttaag ggagatgttt atttcaggtg
     3181 caaacaatct ccaaaacaat aaagagttag ttgacaagtt aaatgtattc cctgtaccag
     3241 atggagatac aggaactaat atgtcattaa caatatctta tgccttaaaa gaattagcaa
     3301 aagttgaaaa tgataatata tcagatatcg gaaaagcatt atctaaaggt tctttaatgg
     3361 gagctagagg aaactcagga gttatacttt cacaaataat cagaggtata gctaaatcta
     3421 tagaaggaaa aagtaaatta tcaacagaag atttagcaaa agcttttaaa aatggttcag
     3481 acacagcata taaagctgta ataaaaccaa ttgaaggaac tatacttact gtagttagag
     3541 aaagtggaga gtttgcaata aaaactgcga aaaaagagaa agatgtagta aagtttttaa
     3601 gtatgcttgt gaaggaatca aattcttcat tagaaagaac tccagacctt ctaaagaatc
     3661 taaaagaagc tggtgtagtt gactctggtg gaaagggtct tgtacttata tatgaaggaa
     3721 tgttagcttc tataaaaggc gataacatag aaataaaaaa tgttgaccta gatactaata
     3781 tttcaacaag tatggatttt gcaaagagta ctactagcac agatgatata aaatattgct
     3841 attgtacaga atttatttta gaaagttcta aggtagaaga tactaaaata agagacataa
     3901 tgatggctta tggtgatagt ctagcagtag ttggagatga tggagtcata aaggtacatg
     3961 tacacactaa tgacccagga aatgtacttc aagaagctct aaagtacggt caattattaa
     4021 ctataaaaat agaaaatatg aagttacaac atgaaaatac attactggat gtagaagaaa
     4081 agaaagaaaa tgatagtgaa cctttagaag aagagaaaga gtttgggttt atagctactt
     4141 ctatgggtga aggtttggca aatatattta aagatttcgg agttgaccat ataatagaag
     4201 gtggtcaaac tatgaatcca agtactgaag attttatgaa tgctataaag catataaatg
     4261 ctaaaaatat atttattttc ccaaataaca gcaatataat aatggctgct aatcaggcaa
     4321 aagaattgag tgataaaaat ataatagtta tacctacaaa aaatacgcct caaggatttg
     4381 ctgcattagt aacttttaat ggagaattaa gcgaagatga aaataaagaa gctatgatga
     4441 atgctttaaa ctcagttaaa tcaggtcaag taacttttgc tgttagggat acagtgatga
     4501 atgaaattga tgttaaagaa ggtaatataa ttggaattgc agaaggaaat ttattatcag
     4561 caggagatta tgtggatgaa gtaacatcaa accttattga aaagttggtt gatgaagata
     4621 ctgctataat aacattattc tttggtgaag atgttacaga atctcaagca aatgaactac
     4681 gtacttcttt agaagaaaag tttgaagatg tagatgtaga gttatattat ggaggacaac
     4741 ctctttatta ttacttaatt tcagttgaat aatgattata agaacctgta tgaccaagta
     4801 gcttttataa aattggttgt acaggatttt tgtgtagtag gtgatatatt tgttggattt
     4861 atataaagat gttcaatatg ttaagggaat aggacctaaa aaagcagata aattaaataa
     4921 actgggtata tttacactaa aagacctatt gtattatttt ccaagacaat ttgaagatag
     4981 aaataatcta aagaaaatag cacaattaga agatggtgag aaagttacga taaaagcagt
     5041 aatatcaagt ataaatacat ttagcccaaa agaaggtatg actttaacaa agattgatgt
     5101 aaaagatgag acaggctcgg ctaaattagt gttttttaat aaaagttata taaaaaatac
     5161 ttttcgtcca ggagattcaa tattagtttt tgggaaggtt aagaaaaaat ttaataactt
     5221 agaacttaca tcatgtgaat tagaatatct tactaattca cctaagaata catgtagatt
     5281 tatgcctgtg taccagctta catacggtgt tacaaataaa gaaattatga gtataattag
     5341 aacagtactt gaagataaag aattaattat acaagaatat atgccacaaa gaattataga
     5401 aaaatatagg ctatgtagta tagattttgc agttagaaac atacattcac caagtagtaa
     5461 ggaatcatta aaaatagctt tatatagaat tgtatttgaa gaattactta tactacaact
     5521 agggttgttt gtatttaaaa gtggcagaaa taaagaagat ggaataaagt ttgaaacaag
     5581 taaagactta aaaaaaataa taagtgcttt accatttaaa ctgactaaag ctcaaaacag
     5641 ggcattagat gaaattatac aagatatgaa tttagagaaa ataatgaata gattggtaca
     5701 aggtgatgta gggagtggta aaacagtagt agctctctta gcacttgcaa attgtgtttt
     5761 aaatggttat cagggcgctt tgatggcacc tacagagata ttagcagggc aacattacat
     5821 ttcacttact gagtctctaa aagattttgg aataaatgta ggtctattaa taggtagtct
     5881 tacaaaaaaa caaaaggata cagtattgga acaaataagg aataatgaga ttgatatatt
     5941 aataggtaca catgcactaa ttgaagataa ggttgaattt aataatattg gtttagttat
     6001 aacagatgaa cagcatagat ttggtgttat gcaaaggagc aaactttcat tgaaaggagc
     6061 aaatcctgat atattagtaa tgacagcaac accaatacca agaactttag cacttatact
     6121 ctatggagat ttagatattt ctattataga tgaacttcct ccaggaagac agccaataga
     6181 aacaattgct attgaaaaaa gtaagcgaga tagagcatat aataaccttg taagaagaga
     6241 ggttgaatca ggaagacaag tgtacattgt atgtcctttg gtagaagaaa gtgaagctat
     6301 agaagctaaa tcagcagttg aacttgtaga ggaattaaga gctgaatatt tccacgattt
     6361 aaggcttgga ttgcttcatg gtaaaatgaa atcgagtgaa aaagacgaag taatggaacg
     6421 ttttaaaaat aaagaaatag atattttagt ttcaacaaca gttatagaag ttggtgtcaa
     6481 tgtcccaaat gcaacattaa tgataataga aaatgcagaa agatttggac ttgcacaatt
     6541 acatcagctt agagggcgag ttggaagagg tagtcataaa tcgtattgtg tactaattta
     6601 tgattctaaa acagatgtat gtagacaaag aatggctata atggaagaga ccaatgatgg
     6661 ttttaagatt tcagagaagg atttagaaat aagaggacct ggagaatttt ttgggacaag
     6721 gcaacatggt cttccagaac ttaaagtagc aaacttattt aaacatataa aaatattgaa
     6781 gttagcacaa caagaagctc gatatatatt gggtgaagat aataatctac aattgaaaga
     6841 aaacatggca ctcaaaaaag aaattataga taaatttaaa gatacattga aagaaatttc
     6901 attaaattaa ttttaaatta tgttaaaata ttactaatgt aataatgtgc tgaagggaga
     6961 ttgtcatatt gagagtaatt tcaggaaaag cgagaggatt aaaactaaat actccaaaaa
     7021 atgaagatgt aaggccaact acagatagag taaaagagtc tctatttaat atgataaatt
     7081 catatataat ggaaagtgaa gttctggatt tatttgctgg gactggttct ttaggaatag
     7141 agtgcttatc taggggtgcc aagtcatgta cttttgtaga tatcagtaag gaaagcatag
     7201 atattgttaa gtccaatgta aaaaaggcaa gagttgaaag tgaaagtatt attttaaacc
     7261 ttgattttaa gactgcaata gataaactta aattacaaaa tagtaaattt gacataattt
     7321 ttatggaccc tccgtattat aaaaatatgt ttatagaagc tattgaaaaa attgataatt
     7381 ctaatctatt gaacgaagat ggaataatag ttgtagaaca tgatacgaat gatttatttc
     7441 cagataagat atgtaaattg gagaaaacta gagataagaa atatggaaat actacattaa
     7501 ctttttataa gatggaggca taatatggaa aataaaccta gaaaagctat atttgcagga
     7561 agttttgacc caattactaa tggacattta gatattattt gcagggcttc gaagttattt
     7621 gacgaattac aaataggagt attaaataat cctaataaaa agggtctatt tagctttgat
     7681 gaaagagtaa aacttataga aaaaagtaca agccatttaa ataatataaa agttgtgact
     7741 tttgatggtt tattaataaa ttactgtcag gaaaatggga ttggagcatt agttagaggt
     7801 gtgagaagtg gtgctgatgt tgattatgaa cttcagatgg cacatatgaa tagagagctt
     7861 aatccagata tagagactat aattttacca agttgtacaa aatattcatt tataagttca
     7921 tctttaataa aagaggtttt gctctttgat gctgatataa aaaatttggt tccaaagatt
     7981 gttttggaag aattaaagaa aaaaactagt gggggtaatt gatatatgaa gatagattta
     8041 gaaatgattg aactatttga gcagttagag gagatattta gaagtgcttc tacaatccct
     8101 ttctcacata aatgcacagt ggataaagat gaggttttag caataattaa tgatataaga
     8161 actttaatac cagaagaagt aacacaggca gtatggatta ataaagaaag aaataaaata
     8221 ataagtcaag ctaagcaaga tgctacaaat atagttgaac aagcgaaaaa agaagctgag
     8281 agaatacaac aagaatatca agataatata gaggaattaa agaaaaattc tgaagatgta
     8341 gtaaaggcat atgtagaatc cagcgaacct gttgttcaag cagaccaaag agcaaaagat
     8401 atagttgata gagctgagag aatagccaat gagataagaa ttgggtcaat tgaatatgca
     8461 gaggatgtgc tttctagtgt tgagtataat ttaagagaaa tattagaaga agtaaaaaga
     8521 gataaagctg aacttagacc taaaaaataa attttaaata aataaaaatg gagctatcac
     8581 acaaaaaatt ccaattgatg tgtgatagct ctcttttaat atagcaattt tttgttatat
     8641 aattgtgctt tttagattac ttatatctta ttttgatatt ataatgtagt ttaatctttt
     8701 atttgttgaa atcaacttat ttatttgtat aaataatata ggttattttc aaattttttt
     8761 aataatttta ccactgtctt cttttagatt agggttagac tgtgcaaaat tataaaataa
     8821 tactaaatca tgtgcaatta tttggtatgg cactacatgt agaaaatata gataataaaa
     8881 ttaaggaggt agtatgaagg aaagggctaa aaaaatatta gattttatta ttaaaaataa
     8941 caatgtaaca tcacaagaac tacaagaaag gtttaatgtt tcaaagagaa ctatttatta
     9001 cgatattcta gctataaatg aacaactagg aaaaagtggt aatataaaaa atgtaaaaca
     9061 caaatttata tttgaaggta atttatgtga tgcaaggaaa ataatttcaa ctgaagaaga
     9121 taaattttta gatagtgatt acagaaaaac ttttatatta aacaagatat tacttggcga
     9181 aaaaatatca atagaaaaac taacaaatga aatgttactt tcaaaaaaca cagtagtaca
     9241 aacaataact gatgtaaaaa aataccttca aacaatgggg ttaagattag agtataaggg
     9301 aaagtacaaa attataggtg atgaatacgt tattagagag ctgtttctga taattgttca
     9361 agaaaatgta ctggaaatta attctataag taaggaagta agtagtttcg atacaaaagg
     9421 acatataaaa cttactgatt attctttatt aaatctaact aagtttgtgg aatttctaaa
     9481 taaaaggata agagatggga aaacattata ctcatataaa tatctaaatg aagctaaaaa
     9541 aattagttac tttagtaatt gtaaagaact tttatgtgaa gaagccaatg aaaatgaaca
     9601 agcctatata tgcacttaca taagttcact tcctagctta aatagtgaag ttaaggaaga
     9661 tgttgttgaa gaatatgtag ataaacttat agataagttt gaagtaaata cagcaataaa
     9721 attagaaagt aagcatgagt ttaagaaaaa tattttaagg cacctccact cttcttacaa
     9781 cagaatacga tttaagtttc ctattagaaa tccaatgtta gatgagacta agtataaaca
     9841 tgagtcactt tataaaatta taaaatcaat tatagaaaat gaagaagagt ttcctgtatt
     9901 tgaggggata agagaagaag agataggatt tatagcagcg tattttggag ggtatttaag
     9961 agggtctagg gataatggcc taagaagaaa taaagtctta ctagtttgtc ctaatggtct
    10021 tatggtatca aagagtttgg aaatacaact atataaatac ataccaacca tagaaatagt
    10081 aggtatagtg tccattaagc aactaaaaga agtaaatgta tactacgatt acataattac
    10141 aactatagat atacaaaatg taaacaatgt aattgttgta aatcctcttt taacatcatc
    10201 agatgttcaa ttacttatga acaaactaat aagtgtaaaa gaaaacgaga agtatttcaa
    10261 tcttgaatta ataatacaag ctataagaaa aaatggagtt atcaataatg aagaagcact
    10321 aaaagctgat ttattaaaca taatacacaa gatagatgaa ggagagatgt atcaaccaat
    10381 gttaaaggaa ttaattaatg cagaaagggt caatataata aaaagtgtta gagattggaa
    10441 agaagcaata aaaatagcct caaagccatt gttagaagat aactctatag aagagttata
    10501 tatagaaaat atgataaagt cagttgaaaa atatggacca tatattgttt tagctgatag
    10561 atttgctcta ccacatgcaa gttctaaaga aggagtaaac aagcttgcta tgtctttact
    10621 aatagtagaa gatgaagttg atttattagg aaaaccagta aatatattta tggtattagc
    10681 tgctgtagat aacactactc acattagagc attagcatca ctatcagaga tgatgtacga
    10741 ggaagaaaat gtaaaactaa taattaatgg tgataaaagt tcaatactag aacttataaa
    10801 taagcaaaat taggaggaat gaaaaatgaa gatattagca gtatgtggtt ttggtgtagg
    10861 ttcatcaatg gtattaaaaa tgacaataca aaaggctcta aagcagttag gtatagaatg
    10921 tgaagtagaa aatacagata taaattcagc tagaggtgct gattgtgact gtatattcac
    10981 aagtcaagaa ttgttaaatg aattaaaagg aacttgtaat gtagatgtat atccagttaa
    11041 aaagtacatg gatttagcag aagtcacaga agttgttcaa acttttttaa gtgataaaaa
    11101 tgataagaaa tagtataagt tttaaaaagt aaatttaagt taatcagaat aaaataaaaa
    11161 cttactaaat tcaaaaatag taagaaagag aggagggatt tagtggtaga aaacttaaac
    11221 agttgaccta tcactaaaaa atataatgct tgattttatt tcaaataata ttttacgtaa
    11281 tccatctatg ttaattggat taatagcagc atttggtctt gtactacaga aaaagaaagc
    11341 atctgatgtt ataaaggggt cactacttgc agctttcgga attataatta tggagactgg
    11401 aacaggtatg ttagttagtt caatagcacc aattaatgga gctttccaaa gtataagtgg
    11461 tggtgcagta acaaaaggtt taagtgatgt tacttttact gcaagttatg gtggaattat
    11521 aggtttagca atgtttgcgg gtttagctat tcacctaatg atagcaagat ttactcctat
    11581 taagacaatc ttcttaacag gtcacatgct ttattggttc ccattcatat ttgtagcttc
    11641 aggagttgaa gctggattaa atggtggagt attaatagct tttgcagcag tattatcagc
    11701 actctattgg tcaataatgc cttggttatt aagaaaatat gtgtttgatg ttataggaga
    11761 tgattctttt acattaggtc atccatctgg ttgtttagca cttatatcag gatttgttgc
    11821 taaattagta ggaaataaag aaaaatctac agaagatata aacatacctc aaagtttatc
    11881 attttttaga gaaatatcta taagtggtgg aatggcagtg tttttaatgt ttatagttgt
    11941 tgggatagta ataccaggtg cgtttaaaga tagtaatgag tttattggaa cgtatgcaat
    12001 aaagcaaggt ctttcttttg gagcaggtct tataataatg cttcatgggg taagaatgct
    12061 aataaatcaa ataatgccag catttcaagg tatatctgaa aaagttgtgc caaatgcact
    12121 acctgctttt gactgtccaa tattatttaa ttataaacca aatgctgtat taataggatt
    12181 cttagttgct atgctaacat ctacagctat gattttaata tgtaactact ttaatgtatt
    12241 taatttgcta cttataccac ttgttataac ttcattcttt gagtgtggag cagcagcagt
    12301 aataggagaa tcacaaggtg gtatacgtgg agctataata ggtacagcta tagctgcaat
    12361 atttatggta gttttagtag gaatatctgc tgtaatttac tcaggaacta ttcaaaattg
    12421 gatactaata tttggaggta acgacctttc attatttggt ataattagta agtttatagg
    12481 ttcactattt ggaggaatat tatagatatg aaatttaaat acatagatga gataagaaga
    12541 ttaattgata taatagaaaa cgaagaaaaa gtcaatatgg aaaaagcagt acaaatcctt
    12601 gttgaagcaa tagaaagtaa aaatgctata ttttcatttg gagcttcaca tgcaggaata
    12661 ttaacagaag aattattcta tagagcaggt ggctgtgttc ttataaatcc agtatttgca
    12721 aaatctctaa tgttagatac agcgcctata actcacacaa gtaagatgga aagattagaa
    12781 ggatatggta cagctctagc tgataaaact ccaattaaaa aaggagatgt agttatagtt
    12841 cattcagtat ctggtagaaa tccagtaagt atagagtttg ctatgaaagc taaggaaaat
    12901 aaggcaatag taatatgtat aactaattta agttactcaa aaagtgtatc ttcaagacat
    12961 tcttcaggaa aaaaacttta tgaagtatgc gatttagtga tagataatca tggtcaaaag
    13021 ggagatgcct gtgtagagat agatggatta aaccaaaaag ttagtccaac ttctactgta
    13081 atagcaacta ctgtgatgaa ctctattata gctcaagcta ctcaggaatt agttaataaa
    13141 gggctaaaga atcctccgat tttctatagt gccaatattg atggtggaga tgagttaaat
    13201 aagaagattt ttgatgaata taaaagtgta attcattatg aatattaagg ttaaaaaaat
    13261 atgttaatga actttgaagt ttatcaacaa agaatattaa taatatataa taaatgctat
    13321 catttaaaag ctgtaggtaa aaatctacag ctttttatta ttgtagatta aaaagatgcg
    13381 taattaaatt ttaaattgga gaaaataaat tggaaggtat caattataaa tattagaaac
    13441 gttgtcatag aaaaaaacta tattaaaaaa aagtacatag aaaaaactga ttagacaata
    13501 aatacatgct ttgataatat tttaaatgga gtgaaaatgt gtagaattat aatgaaaaaa
    13561 gaaaataaaa atgaaaaaag aaaataaaat ggagggaata aaagtgggta tgttaaaaaa
    13621 attatgttca ctagctatgg tagcaattat gactacaaca cttataacag ggtgttcaag
    13681 tggaaatgga aaagattcaa aaaaggatgg cgaaaaagaa aaactagttc tttggatgcc
    13741 accagtagaa aataatatga aggaagtttg ggaccctatt ttagataaat ttgaggaaaa
    13801 aaataactgt gaagtagatt tacagataat tccttgggat aattattctg aaaaatttgc
    13861 aacagctata agtgctggcg aaggtccaga tgtaggatat atgtatgcag aaatgtatcc
    13921 acagtttata gaaagtggag cagtagaaga tttgactaac tatgcaacta aagaggataa
    13981 agagcaatct atttatataa aaacatctga aatgatgggt ggaatgtatg gtatgccatt
    14041 ccaagcagca aatccagggg tattatacta taacaaggat attttagaga aattaggaga
    14101 gaaacctcct aagacatggg aagattttaa aagaatttgt aaaaaagcaa cacaagatac
    14161 tgatggtgat ggaaagatag accaatgggg attagctcag ggatggggag ctaaaacttt
    14221 tggtaactta aactggaact ggtatcctta tttatggcaa gctggtggag atatttttaa
    14281 tgatgattta aaatcggtta aatttaatga taagagtggt ttagaagcag ctaatttctt
    14341 aaaagaatta caagcatatg tgcctgaaga ttctttatct aaagatagta atgaaatgat
    14401 agaaagcgta tttggaccag ggaaagcagc atttacaata atgttatctt ctgctgcaac
    14461 aagtgtattt gatgaatcat tcccggattt aaattggggg tttgtaactg gactagaaga
    14521 taagaaagca gcaacttttg gagcagtaga ccatttatct ttaatgtcat ctgctaaaaa
    14581 taaagaactt gcatacaagt taatacaaca tatgttaagt gttgaatcaa tgacagaatt
    14641 tcataaagca ataccaagag caccaataac aaaaggtgaa ccatatcaag gtgatgaaag
    14701 attcaaagaa atggtggaaa atgacaaaaa tgtatataga ccattagtag taggacctca
    14761 tggagttgaa atatatgaat atttatggaa agaacttcaa actatgattt caggagataa
    14821 aactccaaaa caagcattag atgatgctgc aaaatattca aatgatttat tagcacaata
    14881 aaagtttaaa gctgtaatat gttgtaaagg actttacaga ttaaaattag cattgtattt
    14941 ataaatacaa tgctaatatt tatattggag aggataaaat ggaaaactta gttagagagt
    15001 ttgaagaaaa ttcaaaaaga aaaaaaacaa aattaaatat taaaccatat atgtatatat
    15061 tgccattggg aataatttta gtggcgtttt atgtgatacc aataataatg tcaatatact
    15121 ttagttttac aaagtataat attattagtc cagccacttt tataggttta gaaaattata
    15181 aaaaattatt tacagatgaa acattaaaag tatctattat aaatactatt aaatttactg
    15241 ttgttgtagt accttgccaa actatattat cattgatttt ggcagtttgg attacaggaa
    15301 aaggaaatag taaaatagct tcatttgcta aaggtgctat atttatacca gtattatcat
    15361 ctatggtttt gataggtatg gtatggaggg ctttattgaa tggagaaggt tctattatat
    15421 atcaggtatt aggtgccttt ggaatagaat catctaaact tttaggagat agtaagacag
    15481 cattacctac acttatgttt atatctatgt ggaaaaatat agggtatttc atggtaattt
    15541 atatttcagc tataatgaat ttaccaaaac actgctatga agtagcgaag gttgatgggg
    15601 ctacaaaact tcaagaattt ataaaaataa ctgtaccttt attgaaacca actacaataa
    15661 tggttgtatt tttagggtcg atatggtcat tacaagtatt tgacttaatt tatactgtga
    15721 caggtggagg tccaggtata tctactatga gtatagttat gcatgcattt aacttaaact
    15781 ttaaaaactt taattctgga tatgcaatga ctgtagcaaa cgtattattt ttgttaatag
    15841 cagtagtttc gattttacaa aataagcttg taaaacgaga caattcagat ttttagaaag
    15901 gtaggtataa tatgaaaaca gcaagaagat tattaagtga tggtttactt ttatttatag
    15961 cgtgtgcaag tttggtacct tttatataca tgttgataat atcattgaag ataacatata
    16021 actcatatag tttggatata tctttttcaa cagtaactct tcaaaactat atagatatct
    16081 ttactaaaaa aggatttgca caatattttt ttaatacagc aatagtatct ttttcaggtg
    16141 tattattaaa tttagtattt agtacattag caggatattc ttttgcgaag atggatttta
    16201 aaggtagtga taagttattt ttatttatga taatgacttt aattatacct tcccaagtta
    16261 caatgatacc actatatata ataatgaaac acttaggatg gataaatagt tatcttgcac
    16321 ttattatgcc aataccaact gcatttggcg tatttataat gagacaagct attttaggag
    16381 taccaaaaga gttattagaa tcagcaaaga tagatggttg ctcggatttt agaattttaa
    16441 tacaaatagt attgccttta atcaaaccag cattaattac actagcaata tttactttta
    16501 tgggagcatg gaacgagttt atgtggcctc ttatagctac aactaaagat gcaatgagaa
    16561 cattaacagt tggattatct actcttaaga cattccagat aactaattat ggacagatga
    16621 tggcaggggc tacaataaca tttttacctc catttatttt ctatttgatt ttacaaagta
    16681 aatttgttga aggtgtgtct ttgtctggta taaaaggtta aatagtctaa tttaataaca
    16741 taaaagaaac ttatataaat atggaggaat ttaaactatg gaaatttcat atgtactgaa
    16801 agattgcaca ttcaaacaca ataaatactc agttatagaa gctgaaactt ggaaagagaa
    16861 ggatttggat attgtagtag ctaaaggtga aaaatttgca tttcaaatta tgctaaaagc
    16921 aacagaagaa tttaattgta caattgataa aagcagtact atatcatgga aaggactgga
    16981 aaatagagta agattagcat taaatgtgcc taatagttta gaaaataatt ttagtataaa
    17041 tatattagga tatgttcaag atgatacaaa ggcatttgtg aatgatgcta ttttaaggga
    17101 taaagatgtg ttagtagagc aatatttgcc tcaaacattt tggattgaag gtcaagtacc
    17161 agaagatttt gaagaaaata accttaatat aggtattgat atatttaaaa gctttggata
    17221 tgaagatgaa gaaaaagttt gtacaattga tgttcctgta aaagtaagaa atgtcgtgct
    17281 aaagccttta gataaaagca agttcttttt agatttatgg caacatccaa gttgtttagc
    17341 tagaatgtat aaagttgagt tatggtctga tatacatttt gaaatagtcg ataactattt
    17401 aaaagaatta gcatctttag gagaaaaagt tgcaacagtt atagtttcag attatccatg
    17461 ggcagggcaa agttgttata aagtttataa aaatccatca aacctatatg aatataacat
    17521 ggtaagtgta tcaaaaggat tggatggaaa gattaaatgt aactttgaga gtatggatag
    17581 gtatataagc atagctgata agtataaaat gtctaaggag atagatttat ttggtctatt
    17641 aggtaattgg tgtgctggtg agtttggaaa tcctgtagaa ggttataagg accctataag
    17701 agtaagatat tttgatgaaa atgataaagt atttaaattt ataaacaata caaatgattt
    17761 aaaagaatat ataggattag tattaaatca tttaatagag tgtggattat gggatagagt
    17821 aaggattata gctgatgaac caaataatcc agaagtagtc aaagagtgta tagaatttat
    17881 aaattctact gtaggtactc atcaagttaa atataaatca gcaactcacg accaaaactt
    17941 tttagataga gctaaagatg aaattgatga tatgtcaata aatttaaaac ttactataca
    18001 aaattataaa gatatagaaa atcttaagaa aaaaatcaat gacaagggtg ggatattgac
    18061 ttggtttgta tgctgttttc cagagaaacc aaatagtttt ttaagttctc cttttgtaga
    18121 aaatagaata ataggatggt atacttatta ctttggtctt gatggatttt taagatggga
    18181 ctataattta tggacagaag acccttggaa agattcaagt tacaaattcc ctatttggaa
    18241 agctggagat atgttctttg tttatcctgg aaaagattta aagccagtta gaagtgtcag
    18301 aatggagaat ttaagatttg gaatacagga ctttgagtta tttacaatgt tagaaaaaga
    18361 aaagggcaga gagtatatag aagttgagct tatgcaagaa ttattaaata aaaaagaaaa
    18421 tgctgaaatt aaaggttttg gtgacataga attaggatat tcacttgata attggggata
    18481 tgataaagtt aaaaaatatg tattagagtt gttagacaga gtataaaaaa atataagagt
    18541 gtctcaaaat gaacttttta gttcatgagg cactcttttt gtatgaaatc aatatagttt
    18601 cattgtttca acaatgaaaa ataggattac ttatattcta agatagccct ttagtttttt
    18661 agttgtattc aatgttttaa aactaactat aaacgctata aagtttataa atgacaataa
    18721 ttatatgtaa catgatgtac tttaatttgc tataataggg attgaataat attatatgga
    18781 agataacatt gtttgggggg attaatataa aaagaatagg tattattata atgttttttt
    18841 taagtatagc ttttattgga ttttatcttg caataagagg taacagtgat acagttaaga
    18901 tgcaacgagg gataaatata ggtaatgcac tagaatcacc taaggatttt ccgtgggatg
    18961 taaaaatgtc aaataagttt tttgatgata taaaagatgc aggttttgac acagtacgta
    19021 taccagtaag gttttctgat tatacgtcag attctgataa ttttaaaatt gatgaagaat
    19081 tttttaaaaa gattgataaa tatgtagatt atgcattgga taaagactta atagttgtgt
    19141 tggatttaca tcattttgaa gaaattatga aagaaccaag agttcataaa gaagagtttt
    19201 taaaaatatg gcaacagata gctaataggt atcaaaaata tgataaaaaa ttggtatttg
    19261 aattattaaa tgaacctaaa gaaaatttat cttcacaatt actaaacgaa tacattgaag
    19321 aagcaataaa gataattaga aaaactaacc ctaaaagaac tattatagtg ggaccatata
    19381 atttctatca aattgattat ttgaatgaat taaatgttcc aaaagattct aatatagtag
    19441 tttcttttca ctattacgaa ccaaatgatt ttgcatttca aggaaacatt tatcataatg
    19501 gatttgagca cttgagcaat attacttggg aaggaacaaa tgaacaaatg gattatttaa
    19561 aaaaaaggtt tgatattgta gaaaattggg caaataaaaa taaggtaaaa atattcttgg
    19621 gtgaatttgg agtaacaaaa gaagcaccag aagcttcaag aagagcttgg ataaaagctg
    19681 taagagaaga agctgaaaaa cgaaactttt catgggctta ttgggagctt gcttctggat
    19741 ttggtatata caatcaaatt gaagggactt gggatagaga catattaagt gcattaatag
    19801 aaaagaggtg attttatgaa gaaaaattca aaaaagatac ttataacttt aactattatt
    19861 actaatatcg tatatatatt gtggagaatt ttttacacag tacctaaaga agaagggatg
    19921 tttgccttaa tctgtgctat aatacttctt tttgtagaga taatgggtat gatggaaatg
    19981 tttgtacatt attatggtat gtcaaatatt gagtatcctg aaaaaccagt cataagtgaa
    20041 gaactttatc cacatgtaga tgtattcatt gcaacatata atgagtctgt tgatttggtg
    20101 agaaaaactg taaatggatg tattcatatg cagtatccag ataagaaaaa ggtacatata
    20161 tacatatgtg atgatggaaa tcgtgaagaa atgcgtattc tagcagaaaa aatgggtgta
    20221 aattacataa ctagaactga aagagaagga gcaaaagctg ggaatttaaa taatgcaatg
    20281 caacatacaa actctccttt gattgctacg tttgatgcag atatgatacc aatgcatgac
    20341 tttttaattg ctacagtacc atactttttg aaaaatgagc aagctaaaaa agatggagaa
    20401 aaagaggagt atgaaaaggt tggttttgtg caaactccac agagttttta taatccagac
    20461 ttgttccaat ttaatctgca ctctgaggga aggattccaa atgagcaaga ttatttttac
    20521 agagatattc aattggctag aaataggaca aattctgtaa tttatggtgg tagtaataca
    20581 gttatttcaa gggaagcact tgaagaagtt gatggatttt atacttattc tattacagaa
    20641 gattttgcaa caggtatctt aattcaaagt aaggggtata gatgttatgc tattccagaa
    20701 gtacatgcat caggtttatc tcctacagat ttaaaaagtc ttattaagca acgtgaaaga
    20761 tgggcacgtg gatgtataca aactggtaga cgtctaaata ttttgtttag gagaggttta
    20821 ggtttttggc agaaaataag ttatatttct tcaattactt attggtatgc aagtataaaa
    20881 cgctttgtat atataatggc acctatttta ttttctgtgt ttaatgtgat tgtagtaaaa
    20941 tgtacattac ttcaagtatt ggtattttgg ttgccaatgt atattttaag tagtttatct
    21001 cttaaaatat tttctcaaaa tataagaaat actagatgga ccaatatata tgaaactata
    21061 atgtttcagt ctttaatgcc agcagtaata cttgaaacgt ttgcaatatc taaaaataaa
    21121 ttttcagtaa caaataagaa taaattagaa gaaaatagaa tgtataagtt tttacaggga
    21181 ataccttatt ttatttacat ggtcttatct atcataggaa ttttaaaaat gtttgtagca
    21241 atatttaaaa tgagttcaat gacatattca gttgtattat tttggttgat tggaaacctt
    21301 tttaaccttg ttatggctac attatttata tcagggagac aacagttaag aaagtctgag
    21361 cgttatattg ctgaaattga ttttaagtta aagcaaaact cttatgttct ttcttcaaaa
    21421 acgattgata tatcagaaaa tgggtttgca tttttgctag aaaatccaga atatatttcc
    21481 ccagaagaag agttcgaggt agagtttaga gaaaaatcag gaaatgaaat gtatatagct
    21541 aatatgaaag caaagattgt taatgttgtg gaagtaaatt caaagtggaa atatgcagct
    21601 tatattacac atattgaaga tagtgaaata gataattgga tgtgtattgt tcacgataga
    21661 atacctactt taccgatgac aatatctaat caactaggtt tctttgatga tttgcaaatt
    21721 aatgtaaaaa aacgtattga aaagattaga acattatcac gaagaagtcc aagaatcaat
    21781 atgaattttc agatggatat aaaaaataca ggtaaattaa gaatagtaaa ttttaattat
    21841 caatatgtat tattaaattt tgagaataaa aatatttatc ctaaagaaat agcattggaa
    21901 attaatgaaa atattgtgtt agaatgtgat ttatgtgagg gtaaaatcga tgaaagaggt
    21961 ctcttatata aagtaaataa cattgacagc ataatgcaaa atttcttttt aagagatgaa
    22021 atgatggatt ggatattaca aaataagaca attcctgttt caaatcctag tgaaaagaag
    22081 gaaaaaagta tagatgagtt tgaacccatg gaatatattt aggagagatg aaatgaagac
    22141 tttaaaagtg tttttttgtt ctgcaaaata tcttattttg gtgtttattt tttttataaa
    22201 tactactttt ataagttcag cgacaagtga taaaatttta g
//
LOCUS       DAEPLF010000044        21080 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEPLF010000044 DAEPLF010000000
VERSION     DAEPLF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21080
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.044"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(128..1033)
                     /locus_tag="KR314_001468"
     CDS             complement(128..1033)
                     /locus_tag="KR314_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HBH0327859.1"
                     /translation="MESKEKSILRATHFGIIKIGEKDLNCAVLENGSRIINKSTVFKA
                     FGRTKRGRKKDEIRVPSKPNLPSFIDAKNLSDYISDELEDMLINTIKYKTKNGRIVEG
                     YPAEIIPLICDVYLQARLDNKLAVQQEGLAIASEILVRSLSKIGIVALVDEATGYQYD
                     RDRDELQKILSMYISKEFLPWTKRFPDEFYRQMFRLKNWTYPRPSSKRPGIVGTYTNK
                     YVYDLLPPGVKEELQRVNPTVKPGQRKHKHHQFLTEDIGNDHLKNHLLKVITLMQASK
                     DWKDFNVLFNRAFNLPVQLEIEYDE"
     gene            1944..2159
                     /locus_tag="KR314_001469"
     CDS             1944..2159
                     /locus_tag="KR314_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBH0327860.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKSYIVEDLIQVLEEKGVM"
     gene            2159..2638
                     /locus_tag="KR314_001470"
     CDS             2159..2638
                     /locus_tag="KR314_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBH0327861.1"
                     /translation="MIVYHDSYCVAKTPNIIFLRDSLDFGKGFYVTSIKTQAINWIER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFNSYNLEWLDFILECRNMSDVYMKYDVIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRCEEEV"
     gene            2644..2874
                     /locus_tag="KR314_001471"
     CDS             2644..2874
                     /locus_tag="KR314_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327862.1"
                     /translation="MEANKTLLQILYKKIILEFSNQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(2990..4156)
                     /locus_tag="KR314_001472"
     CDS             complement(2990..4156)
                     /locus_tag="KR314_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH0327863.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHMQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENRLEILTLEQQKNFIKAIEGHKLEVLFLVALGTGLRLGELLGLKW
                     FDIDFKNSNLTVKRTLQRTYFIDKNGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNVDILKAGELYRNDNYVFCNELGVPIDDKRPLRNLKSILSSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(4146..4898)
                     /locus_tag="KR314_001473"
     CDS             complement(4146..4898)
                     /locus_tag="KR314_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBH0327864.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYIIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNNLFISDISIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            complement(4990..5481)
                     /locus_tag="KR314_001474"
     CDS             complement(4990..5481)
                     /locus_tag="KR314_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0327865.1"
                     /translation="MINNTIGEKIKQYRKSINMSRNELANKIGCSVHAIAKYEQNQRI
                     PSLDMIRKISTALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVVHILENYGYDI
                     KTDYKKKFEITDNADTIKPISLSFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            complement(5732..6496)
                     /locus_tag="KR314_001475"
     CDS             complement(5732..6496)
                     /locus_tag="KR314_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0327866.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYFLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCVSNTELDFSLHPDDI
                     KEIIKNDVLIKQSIDELLDIGLSEENIEAIYDYFSEATDNGSNIRLKKLISQKENMTI
                     KNNDYFKLLQENNELKEKNKKLENTLKEIKKLLTKI"
     gene            6643..6843
                     /locus_tag="KR314_001476"
     CDS             6643..6843
                     /locus_tag="KR314_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0327867.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKEE"
     gene            6847..7161
                     /locus_tag="KR314_001477"
     CDS             6847..7161
                     /locus_tag="KR314_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327868.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            7183..7926
                     /locus_tag="KR314_001478"
     CDS             7183..7926
                     /locus_tag="KR314_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327869.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKRIKIKSGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSIIREYDKEAVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYHSKEIVINKETGIYNPDDSQLKW"
     gene            7939..9273
                     /locus_tag="KR314_001479"
     CDS             7939..9273
                     /locus_tag="KR314_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0327870.1"
                     /translation="MNNSNNLIKNTEAEQSILGSILLDSTIVEELEQMLNEDMFYHEE
                     HKYIFNAIKEINKRNEVIDIITLTEELKRKGHLNNIGGISYITSLSTIVPTVSNFTSY
                     VSIVIEKYKNRNIIDKFNKFNLGEIDDNTLIYQLENDINKANQKLIKKDSSITAISER
                     AFEYINDEMNEGIKTGIKFLDDTIGGLYGGELTTIAAKSGRGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNDDEIVKYIQALSIINQENNLY
                     INDSVNTVSGIKREIKSTKPSLVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPVIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALNYCKTMGIE
                     DIRELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGNLFSFEECKKIQEI"
     gene            9523..9795
                     /locus_tag="KR314_001480"
     CDS             9523..9795
                     /locus_tag="KR314_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327871.1"
                     /translation="MYDEIIEIYFDYWINEHMYRSILEKDEYYIECWKEVKKINELLE
                     INLEDDKKYLLVELNDLFNSLIEFERELSYKLGFKTGAKFIIELLK"
     gene            10068..11195
                     /locus_tag="KR314_001481"
     CDS             10068..11195
                     /locus_tag="KR314_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBH0327872.1"
                     /translation="MIIPKLEPNDRKREFNKYSSEQINKVVYNYLFKNNMSNRKIDDE
                     ILDLSSKQSKGYQSMGIVHHLGINKNHKGIFTGYTIDKAIEVLKEDEIHFKQIINILL
                     EIRNINEYPIIVDSWELLDEKIALKTIDKSTLKHNGTGVPIEIRYFFEINNLGKNEKK
                     NVNFKYNDNYYSAVIEMDKLENPRSRIIWKSKFRDLLRSIFSEYYEKFLENKIEGKTN
                     LPKIRFIKLDSNNFNIEFIFTAEIEDDVLEEISQDNPLNINTINIELETEIRKEGKVK
                     YVYGKQYERNPRNRIEAIKYHGTKCIVCGFDFEKTYGDRGKGYIEIHHIKPLSSVGEE
                     SNINPKTDLVPICSNCHRMIHRKKDNVLSIDDLKQIIKDSK"
     gene            11675..12322
                     /gene="cas5"
                     /locus_tag="KR314_001482"
     CDS             11675..12322
                     /gene="cas5"
                     /locus_tag="KR314_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBH0327873.1"
                     /translation="MILNKIEIIGDMAHFKIPMHSKIQRTYEIPPISTVVGILKNIYG
                     EEIDDFILGYVIYHDGTFKDLQTIYKEINPNVKTLTDSDRFQTDVCPIEYLINPKLII
                     YTNIDKNIEFNEPLCLGKTNCLAKVLSLSEIEVNLIDKKAIGYNQYTDINIGDGMIKR
                     INTLTEYNEKKGYYDIYSSMVRENDEFEYGKYYDEDEQQNIFLWKWKKGGVIKCI"
     gene            12313..13077
                     /gene="cas6"
                     /locus_tag="KR314_001483"
     CDS             12313..13077
                     /gene="cas6"
                     /locus_tag="KR314_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBH0327874.1"
                     /translation="MHLVRRHLILTVDNEVVLDYNYQYELMKRIYEAIEINDKRKALS
                     LHNEGYKVDKKVFKLFNYTIMFENAKYLKEGIHLNPQTKIKLILSGYDDILNNIIKGF
                     IKCKVFKLNNIEFKVSDIEEDSKKNFNNITLYKVRSPIVASLYDLKSRKQVYLNPMQE
                     EFYKALHDNLGNKYKLIHNKEYTGELYFDIEDVLAVKKKYITNIKGKGFIIGYTDFEI
                     FVQANKDMQEVIYYCGLGEKNSIGMGLLTYITSRRA"
     gene            13079..14245
                     /locus_tag="KR314_001484"
     CDS             13079..14245
                     /locus_tag="KR314_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327875.1"
                     /translation="MDRSYNVFVDNGLYVLAYYLNKDINDITYQDIENSIDLMSDKIE
                     EFVSCEKYSNLKSMCFSNSTLTQPKGKVTLNEKLKGFVKNQGDEYCSLCGQYKAKVKI
                     EDKEYNIGRSYMPNLVANTFYNFSNNLQGLNVCPYCLVLTMYSILNCRVSRYAFLYNS
                     TSSEFMEDYTFHIQKENLTDVELGAKKEKEKYSIVKLLESLVCKYNLFNGDIEQYMFN
                     NSGQSQDIQVNRIKNKYVNLLIKLQEKALLSNFEKLHLDRYILNGTLESNYLREVYRV
                     KKEEKMDEKEQKNFDELVIFLNREVNVLKKVKIDIIKKLTDKLKTSDLDIDKIYKELK
                     MLGKFYAYEDWLVNLNEMYYEKTGEKLFEAEDYIELDDIRSFRQIKNLILVSLI"
     gene            14266..15336
                     /locus_tag="KR314_001485"
     CDS             14266..15336
                     /locus_tag="KR314_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HBH0327876.1"
                     /translation="MENKNSNRMLNIGYAIEVLCGRLNGSSSEVEKKNNSYKNSTYTK
                     KIGSRSRISSQCQKYNQKLFMEQVCGYEQNVKTKDGKQVKLVPNPFKYATDDIFGFMM
                     AEKLSIEEDVYKELSDEEKEIYKKNKKKYEVNITKKRKSRWQMNSLINVSNRKVEWEW
                     NVCGTTGDSMPYQVEVYSGVHTNIANLNIGKIGEYDISDIASEHRDYTTLEAKSIGVE
                     KLDKEEKFKRIEALLKSLEYLSVEGNKTNHLTDTKPKFVILGEYSWGNNVFQGIIKEN
                     GLDVEMLKESIEQNEEFRKSDIWIGINKFYDDTFNEIEVNNEKEQIKKYLEEEFEEYD
                     FMKISNVHQAFESYLKYLKETL"
     gene            15347..17332
                     /locus_tag="KR314_001486"
     CDS             15347..17332
                     /locus_tag="KR314_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBH0327877.1"
                     /translation="MNNLYEYKAKSDGTSIDKHNEDMNYIMNQLVSIYNISNDMKNDL
                     YEAIVCHDVGKVVKSFQDNIESSTRRVRHELLSASIKYLNRGQKLAILTHHKDIKFLE
                     GQITNSIYEIELKELKELLDIEVEDIRPFIKEISRARNDDTRKLENILLKGYLQYCDH
                     IASAGIKSIDKGFNSIKDFSIPKGVSYNSIQEKVLNMDKNEDIMIMAMTGLGKTATSL
                     YWSNLVQNEYKSKRVYYILPYTASINSLYENLYNRNISVSMLHSKAPYFLNKISDEED
                     IKQLYQIYKKSVKQINVCTIFQLVKSVFSCKRFEMLLSQLKDSIFIVDEIHCFDTRVL
                     CYTLEFLKYLKENLNVKICIMSASIPTCLKKLIQERLQISTIIEPDSKDLIFRHRLNR
                     LSEGILDSIHKIENDLMQGKRVLICVNSVDLSQELYGRLQKYNPKLIHGRFNTRDREQ
                     AEKGIEESFLLIGTQSIEVSLDISYDVLYTELAPLDSLLQRFGRVNRRGDNAELSNIY
                     IYDYDRKLNIYDEDIINRTDIVISDIIHNDNSIVDESKVPSYLDNVYLDIDMDKYNKH
                     KVALEIIMKNMRLGVWNKSANNDMLNNDTISVVPECLLEEYKLYIDKKMYLEASSLFV
                     NVSVKRYNYNKKMFYECGDIVVTSYSYNDKLGLIFDC"
     repeat_region   17585..18207
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17585..17613
                     /rpt_unit_seq="gtttaaattacactaagttagttataaat"
     gene            18652..20208
                     /locus_tag="KR314_001487"
                     /pseudo
     CDS             18652..20208
                     /locus_tag="KR314_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444896.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         8463 a         2147 c         3217 g         7253 t
ORIGIN      
        1 atatataaaa tttaaataat atatcataaa agtacctata ctaataagta cttttatgat
       61 ataagttaat tctattttta taatataaat taaaagtatt taaagcgttg ttaaaggcta
      121 tagtctatta ttcatcatat tcgatttcga gctgtacagg tagattgaaa gctctattaa
      181 aaagcacatt gaaatctttc cagtcttttg atgcttgcat taaggttata acctttaata
      241 aatgattttt taaatgatca ttacctatat cttctgttaa aaattgatga tgtttatgtt
      301 ttctttgccc aggttttact gtggggttaa ctctttgaag ttcttcttta actccaggag
      361 gtaataaatc ataaacatat ttatttgtgt atgttccaac tattccaggt ctcttagaac
      421 ttggtctagg gtatgtccag ttttttaatc tgaacatttg tctataaaat tcatcaggaa
      481 atctttttgt ccaaggtaaa aactctttag aaatatacat agacaatatt ttttgcagct
      541 catcacgatc tctatcatat tgatatcctg tagcttcatc aactaatgct acaatcccta
      601 ttttggataa acttcttact aaaatttcgc ttgctatagc tagtccttct tgctgaacag
      661 ctaatttatt atcaagcctt gcttgtaaat atacatcaca tataagaggt attatttcag
      721 ctggataacc ttccacaatt cttccgtttt tagtcttata ttttattgta tttataagca
      781 tatcttctaa ttcatctgaa atataatcag atagattctt tgcatcaata aaacttggta
      841 gattaggctt agaaggtaca cgaatttcat ctttttttct accacgcttt gttcttccaa
      901 aagctttgaa tactgtactt ttatttatga ttcttgaacc attttctagt acagcacaat
      961 ttaaatcttt ttcacctatt tttataatac caaaatgtgt tgctctcaat atactttttt
     1021 cttttgattc caatttaaca ccatccttta ttttatttta ataagtaaga tattacttag
     1081 tatctcttca ttagataaat tagtatttat aagactaata atatcaattt taaaatatgt
     1141 atatagataa ttgagttttt ttatcatctt gtacgcagta tttttattaa caacgatatt
     1201 ttttgataat tttctatagc taatattttc atcaaatata aataaattaa tagcaagtag
     1261 ccattttctg tagtctagtt tagttttatg cataatggta ttagttgtaa ttgaaaactt
     1321 actattgcaa atattgcaat gaaataaatc attataagta ctaattttat cagaataaca
     1381 ataggggcat attaatgagt tttttaattt aagattatat aaaaaaatct tataagagac
     1441 atctgttgga aagtttttat aaatatcaaa gtcttttgtc ataatttaat tatatcatgt
     1501 tgtagaaatt aatttactgt atttaagtgg gtcaaattta atatatatgt ttttgatagt
     1561 gtaaaaatta aaatataatt ttaggcatgt ttgtattgaa taatgagcac tttcaaattt
     1621 tattagtgta agaggtttca aattataaag taatgaatgt atattactat atgatttata
     1681 attagaataa aatttttata atagaaattt aatatatcaa aatatcagtt gaggatattg
     1741 ttcaagtggt atatcttccc tgagttaaaa aataaatgat gaaatatttg gaaatgctag
     1801 tctttatttg tttatgatat tatgtaacat agaattttaa tatctacttt atgatataat
     1861 atttttataa acataagatg gtttaagtta ttaattaata aaaatatata tattatataa
     1921 gtggatagga gttgaggttt gtaatggatg aaagaaaact agaattttct attttttgca
     1981 ttgaagcttt agcagaaaag ttgaatatag attcagaagg agtttataaa cttcttaaac
     2041 atgatactga tattttagat aactatataa taccttgcta tgaagcacta cattctcaaa
     2101 gtaaaagtta tatagtggaa gatttaatac aagtgcttga agaaaagggc gtgatgtaat
     2161 aattgtatat catgattcat actgtgtggc aaaaacgcct aatattatct tcttaagaga
     2221 ttctttagat tttggaaaag gattctatgt aaccagtata aaaacacagg caattaactg
     2281 gatagaacgt tttaagaaaa ggggtaaaaa agcatattta aatagctatt ctctagatat
     2341 tgataaatta aaagaaaact gcaaggtaaa aatcttcaat agttataatt tagaatggct
     2401 tgactttata ttagaatgta gaaatatgag tgatgtatat atgaaatatg atgttataat
     2461 aggtggaatt gctgatgata agatttacaa tacaatagaa ctgtatcaag ataatcttat
     2521 tgaaaaagat gaagctttaa aaagattaaa atactataag ccaaatgaac aaatatgtat
     2581 aactaatcaa aatgtaatag ataaatattt aaagtttaga tgtgaagagg aggtatagtg
     2641 cttatggaag caaataaaac attattacag atactatata aaaaaattat attagaattt
     2701 tctaatcaga caggcaagag tttggaagaa agtatggatt atttatatac ttctgaaaca
     2761 tataaactta taagtgaggg tgtatcagat atgcactgta agggtcatat atacttggct
     2821 caagaattaa tgctagaaaa tggattaatg tatcataaag gctatccaag ataaatagtt
     2881 aaaactagat acgaattaag gatacaagat aatataaaat taatttgtat ccttatttgg
     2941 gttttgtaat taactatatg aaatcatagt atttttatgt atttgatttt tacaatgaaa
     3001 atattttatc caacttttct acagcttcaa ccttcttatc ttccataact tgagtatata
     3061 tatttaaagt tattgatata tcataatgac ccattaaaac ttgtacagtt ttaggaggaa
     3121 catcattctc aaaaagtcta gttgcataag tttttctaag accatgaaac tttataggtt
     3181 ctatatctaa gctgcttaat atagatttta aattccttaa aggtctttta tcatctatag
     3241 ggacacctaa ttcattgcaa aatacataat tatcatttct atataattct ccagccttta
     3301 atatatccac attttgattt actttatgct ctttcaattt atttaaaaca tcttttggaa
     3361 taggtacagt cctgtaagaa tttgatgtct ttggttcttg ctctaatact ttaagctctc
     3421 tatttccatt tttatctata aaatatgttc tttgaagggt tcttttaact gttaaattag
     3481 aatttttaaa atcaatatca aaccatttaa gtcctaataa ttctcctaat ctaagtccag
     3541 taccaagagc aaccaaaaat aatacttcta atttatgccc ctctatagct tttataaaat
     3601 tcttttgttg ttcaagagtc aatatttcaa gcctattttc ctttttctca actggtatat
     3661 ttaccatagt acaataatta cgttgtataa gaccttgttt ttcagcttct gataagcagg
     3721 ttttcagttt agtgtttatt tgccttatag taggtatact tttattggaa tcaagcaatc
     3781 tattgtaata tttttgcatg tgagatactc ttaagtcctt taacttaaca ttgcctaaat
     3841 cagtatcttt aatataatta cgatagatgc tttcatatct ttcaaatgac tttggtttta
     3901 aatcttttat tctatattca aacaaccaat tgtaaaacca atgctctaaa gttattttat
     3961 catctgaaat atcagaactt aaataatatt gttttttata agcatcaagt ttatctttaa
     4021 cttccttttg agttttacca taaaattctt ttcttattgg ttttccatct tcattataac
     4081 caatcattat acgagaacgc caaccatctt tatattttcc gatagagcct tctccattag
     4141 ctcttttagt tgccattttt aaacaactcc ttatatgatt tatagttata tccatcgaag
     4201 aatgtaatac atttctctaa taagaatttt ataacttcat cagcttctag tgtttcatag
     4261 aagtttagca ttaatgtgtt gtattcttgt ttatgtttgg ctggtattga tagaactcct
     4321 atatcattac ttataagtat taggtttgag gtaagtcgag cagttctttt gttaccatca
     4381 tagaagaatt gacaatagga caaccataaa tttattatta tagccttttc taaggggtta
     4441 gttattttat ttatgatact tatgtcactt ataaataaat tatttaatag agtggcttct
     4501 atgcactcat attgagtagt accagctata cctatgttac cgtttctaaa atctccaaca
     4561 attagagctt catcttttgc aactatttta tgtatagagc aaattgtatc tttatttaaa
     4621 gataagtttt ctttgttact taattttatt atataatccc atgatgattt tatatttagt
     4681 atttgatttt ggtcacttaa tttatgtcca cctactgtta taccatcaag taatgtttgt
     4741 acttctggga atgtgaaggg gttattttct aggttaccca tatcatatac aaattcactt
     4801 atcattcttc taatgtatgt taaagtatac tttttatctc catgaggtag attcgtaggg
     4861 aatttatgtg tgtattttgg tatatatgat ttattcatga ttttagctcc tttcatatct
     4921 aatttttatt tattaactat gaatgtataa aagttaatag gtaaacccat attttgaaaa
     4981 tgtgcaatac taatattctt catcctcatc ttcaataaga ccattagagt tttcttgtat
     5041 aaaattttca acaagaccat ttatcaccca atttagattt ttggagaagt ctttgaagtc
     5101 agagaagctt aagctaattg gttttattgt atctgcatta tctgtaattt caaacttttt
     5161 tttatagtct gtttttatat cataaccata attttctaat atgtgaacta ctaaatccaa
     5221 ctctttatca aatactttta aattaatatt attatttgtt aaagtatcta aatcattttc
     5281 ttcaacaatg agtttattta tggcaattga taaagcagta gatatttttc ttatcatatc
     5341 tagactaggt attctttgat tttgctcata tttagctata gcatgaactg aacaaccaat
     5401 cttgttggct agctcattcc ttgacatatt tatcgatttc ctatattgtt taatcttttc
     5461 tccgattgta ttatttatca taattccacc ttaaatatat catcaaacaa tgattgtatt
     5521 tttacacata taaaggagtt attttctaga ttatctatat catatactaa cttactcata
     5581 attcttttaa tgtatgttag agtatatttt ttatctccat aaaataaatt agtagggtat
     5641 ttatatgtgt attttagcat atatgcttta ttaataattg taactccatt tgtagttttt
     5701 tatcattgct ttagatagta ttgatattat tttatatttt agttagtagt ttttttattt
     5761 ctttaagtgt attttcaagc tttttatttt tttcctttaa ttcattattt tcttgtaata
     5821 atttaaagta atcattattt ttaatagtca tattttcttt ttgagatatt aactttttta
     5881 gtcgaatgtt actcccatta tctgttgcct cactaaagta atcatagatt gcttctatat
     5941 tttcttctga tagaccaata tctaataatt catcaattga ttgttttata agaacatcat
     6001 ttttaataat ttcttttata tcatcaggat gtaatgaaaa atctaattca gtattagata
     6061 cacaatctga aagtatccaa ttataaactt gattttcatt tagttctaat ttttcaccaa
     6121 gtttataata ttggtcttta tcagaccaag cacctagcaa taaacttgag gtaatcctaa
     6181 tatcatcatc ttccataaga tttgaaatag catttaaaaa ttcatcaaaa gaatagtatt
     6241 cttctgggaa taaagactgt aaaaaatata aaacatcttc attagtcatt acttcatttt
     6301 caatatcaaa ataaaagata gtcttttcaa tttttaaagc tttagcgagt tttgataagg
     6361 tatctatact aggtattctt ttatcatttt cataatttat tagagcattt cttgagatgc
     6421 cactaatttc agcaagttga atctgtgtta gttctttttg ttttcttatc ttttttaagt
     6481 tttttccaat ttgcaatatt atcacctcgt ataaagtata tcccaaaagt acacaaatga
     6541 gaacagaaaa ataatatatt cttattgaca gcacacatat gtcgacatta taattaaaat
     6601 gtaaagttta cgaatgtaaa ctagaaaaaa ggaggaaaaa gtatgggaat caatttaaaa
     6661 atatttagga ttaaaaaagg tttaaagcaa caagaactgg cagatagagt tggtatatca
     6721 agatattatt tgtcaaactt ggaaacaggt aaggctaata atccaagcaa tgatttgatg
     6781 attaaactat ctagagcttt agatacaaca gtagaagaat tattttttag taaggaggag
     6841 taaaatatga aagagaataa taatttagaa gttttaaaga cattacaagc tactttaaac
     6901 attattgtaa agaatgaaga agaagcacaa gaaaaaaagc aaggatataa aattattgaa
     6961 aatgcgaaaa gaaataaagc tgaacttata acatgtaaag aagcatctaa gctttatcct
     7021 ataggagaat caaaatttag gcagttatgt cactctaaaa ataaaggttt cccttgtata
     7081 tggataaata atcgagtata cataataaaa aacaaattgg atgattggtt tattgaaaat
     7141 gcaaatggta tcaagttata gtcttaaagt aggtgtttgc aattgagcaa taaagggtgg
     7201 ataaagcttt acagggagtt gttggataaa tcaatttgga aagatattaa accagaaaga
     7261 aaagtaatat taataactct attacttatg gctagtcata aagaaaatca tgtttttata
     7321 gataataaaa ggataaaaat aaagtctgga caatttattt cttcttatag caaaatagct
     7381 gaaagatgtg gtaaaggagt tagttatcaa atggttagaa gttgtttgtt ggtttttaaa
     7441 aatgctggtt ttctaacata cgaaacaaca aacaaatata tagtcataac tattgaaaac
     7501 tgggagcttt accaagagaa catagaaaat caacaaccta atcaacagac gattaacaat
     7561 caatcaacaa caatcaagaa tataaagaat gaaaataata ttatatatag tgaaattata
     7621 agcaaattta actcaacttg tattgaactt tcaaaggtca agaaactaac agacacaaga
     7681 aagaggagaa taaattcaat aataagagaa tatgataaag aagctgtatt agaggttata
     7741 aatatagtta gtaaaacaag ttttctgaat ggaaataata atacaggatg gaaagcaaat
     7801 tttgattgga taatgaattc aagtaatttt accaagatac tagagggcaa ttatcatagc
     7861 aaggaaattg tcataaataa agagacagga atatataatc cagatgattc acaattaaag
     7921 tggtaaggag gttatattat aaataattca aacaacttaa ttaaaaatac agaagcagag
     7981 caatcaatat taggaagcat tttattagac tcaacaatag tagaagagtt agagcaaatg
     8041 ttaaatgaag acatgtttta tcatgaggaa cataaataca tatttaatgc tataaaagaa
     8101 ataaataaga gaaatgaagt aatagatata ataactctta cagaagaatt aaagagaaaa
     8161 ggtcatttga ataatattgg aggtataagc tatattacca gtttgtcaac aatagtccca
     8221 acagtatcaa atttcacgag ttatgtcagt atagttattg aaaaatataa aaatagaaat
     8281 ataattgata aattcaataa gttcaatctt ggagaaatag atgataatac tcttatatat
     8341 cagttagaaa atgatataaa taaagccaat cagaaattaa taaaaaagga ttcaagtata
     8401 acagccatat cagagagagc ctttgaatat ataaatgatg aaatgaatga aggtattaag
     8461 actggaataa agttcttaga tgatactata ggaggtttat atggaggtga actaactaca
     8521 atagcagcca aaagtggaag ggggaaaaca gcattagctt tacaaatact tagaaatgta
     8581 atatttcaag gaaagaaagt actttttata agtggagaaa tgagtgatat acagattctg
     8641 tttagaaata tagcctcact aactggaata tcagttgtaa aaatgaaaaa tagaaatcta
     8701 aatgatgatg aaatagtcaa atatatacaa gctttaagca taataaatca agaaaataac
     8761 ctttatataa atgactctgt aaatacagta tcagggataa agagagaaat aaagagtact
     8821 aagcctagct tagtcataat tgattatttg caaattttgg attatgaagg taaagaagta
     8881 ggagaagcta aatttgctga cttatcaagg caaataaaaa agattacttt agattttaat
     8941 ataccagtta ttcagcttgc tcaattaaat gatgaattga aggatgaaag acctaaaggg
     9001 gatagagtaa tgagaagctc aaagcagata tatatggatt ctaattcagt tatatatatt
     9061 catagaccta cagaaaaaga ggctcttaat tattgcaaga caatgggaat tgaggatata
     9121 agagagttga gacgaagaga aaaaggtttt ggtgctaatt taagtgagat tattgttgcc
     9181 aaaaatagag atggtgagtc aggatttaga ccatattggt atataggaaa tttgttttct
     9241 tttgaagaat gtaagaagat acaagagata taaaaaagaa gcatgattta ttaagtaata
     9301 gataaattat gtttcaagaa gtattgaatg gtaaatatat aaaacttaat tagatgtaat
     9361 agagatttaa aatatattga gtttagattt aaatgcttat tagaaaaagg tagttgccat
     9421 ataaatataa caactacctt tataaattag gtacttaatg tacactaacc tagcaactta
     9481 agtatatatt aagtacctga tgaaagtcaa ggaggtacta aaatgtatga tgaaattata
     9541 gaaatatatt ttgattattg gataaatgaa catatgtata gaagtatttt agaaaaagat
     9601 gagtattata ttgaatgttg gaaagaggtt aaaaaaatta atgagttatt ggaaataaat
     9661 ttggaagatg ataagaaata tctgttggtt gaattaaatg atttgtttaa ttcattaata
     9721 gaatttgaaa gagaacttag ttataaatta ggatttaaaa caggagcaaa atttataata
     9781 gagttgttga agtaattaat ttgtgtatct caatagaata attaaatagt aaatcgagat
     9841 tattaataag ttgtactaga atattttaag tgaagtatat attaattata ttgtttaaaa
     9901 taaatatatt taataaagag gggataagta tgccctcttt tatttttatg caatcaatga
     9961 cattgtagga tttacgtatt ttgataaata gcacaatata ttttaataat aaatatatga
    10021 tacaataatt ttattaaaat ctaaaataat caattgagga ggagaatatg ataataccta
    10081 agttagagcc taatgataga aaaagagagt ttaataaata ctcatcagag caaataaaca
    10141 aagtagttta taattattta tttaaaaata atatgagtaa tagaaagata gatgatgaga
    10201 tactagattt atcatcaaaa caaagtaaag gttatcaatc aatgggtata gtgcatcatt
    10261 taggaataaa taaaaatcat aaaggtattt ttacaggata cacaatagat aaagcaattg
    10321 aagttcttaa ggaagatgag atacatttca agcagattat taatatactt ttagaaatta
    10381 gaaatataaa tgagtatcct ataatagttg actcttggga gttattagat gagaaaatag
    10441 ctttaaaaac aattgataaa tctacattaa aacataatgg gacaggagtt ccaatagaaa
    10501 taagatattt ttttgagata aataatttag gaaagaatga aaagaaaaat gtgaatttta
    10561 aatataatga taattattat agtgcagtta tagagatgga taaattagag aatccaagaa
    10621 gtagaataat ttggaaatct aagtttagag atttactaag aagtatattt tctgagtatt
    10681 atgagaaatt cttagaaaat aaaatagaag gaaaaacaaa tttacctaaa ataagattca
    10741 taaaacttga tagtaataat tttaacatag aattcatttt tactgctgaa atagaagacg
    10801 atgtattaga ggagataagt caagataatc ctttgaacat taatactatt aatatagaat
    10861 tagaaactga aattagaaaa gaagggaaag ttaaatatgt ttatgggaaa cagtatgagc
    10921 gtaaccccag aaatagaata gaagcaataa aatatcatgg cacaaagtgt attgtatgtg
    10981 ggtttgactt cgaaaaaacg tatggagata gaggtaaggg atacatagaa attcatcata
    11041 ttaaaccatt aagttctgtt ggagaagaaa gcaatataaa tcccaaaaca gatttggtgc
    11101 caatatgttc aaattgtcat agaatgattc acaggaagaa agataatgtt ttgagtattg
    11161 atgatttgaa gcaaattata aaagatagta aataatgctt atgtgtggca gagatgtata
    11221 aagtatatat tctaaactag ttaacaacaa atactaatat aaaaaatcaa aaaatttatt
    11281 aggaaaaggt agttgctaaa gtataacagc tacttttata aattaagtat ttgaggtata
    11341 ttagtctaac cgattaggta tatcttaagt tattgatgaa aattaattaa gactaacatg
    11401 tatgatgaat ccatagaaat gtatttttat tgttaaataa atgaacatac atagaaatat
    11461 tttagaaaat tacaagtact atattgaaag tttgaaggag aaccttgatt taaggtaagt
    11521 gtaaagtttt tagaaacttc actaaaataa tctaattaag ttatatattt gttactttta
    11581 ggaaaataat ggtaaaaatt aaaggttttt ataaatgaat gtctaatttt ataaataaaa
    11641 tggagtattt taactaaata tttgaggaga taaaatgata ttaaataaaa tcgaaataat
    11701 tggagatatg gctcacttta aaattcctat gcatagcaag atacaaagaa catatgaaat
    11761 tccaccaata tcaacagttg tcggaatttt aaaaaatata tatggtgaag aaatagatga
    11821 ttttatatta ggatatgtta tatatcatga tggtacattt aaagatttac agactatata
    11881 taaagaaatc aatccaaatg taaaaacatt gacagatagc gatagatttc aaactgatgt
    11941 atgtcccatt gaatacctaa ttaatcctaa acttatcata tatactaaca tagataaaaa
    12001 tatagaattt aatgagccgc tatgtttagg taagactaat tgtttagcga aggtattatc
    12061 tttaagtgaa attgaagtaa atctaataga taaaaaagct attggataca atcaatatac
    12121 agatattaat attggagatg gaatgataaa aagaataaat acattgacag agtataatga
    12181 aaaaaaaggt tattatgata tatattcttc aatggtaaga gaaaatgatg agtttgaata
    12241 tggtaaatat tatgatgaag atgagcaaca gaatattttt ttatggaaat ggaaaaaagg
    12301 gggagtaatt aaatgcattt agtaagaaga catttgatac tcacagtgga taatgaagtt
    12361 gtattagatt acaattacca gtatgagcta atgaaaagga tatatgaagc tatagaaatt
    12421 aatgataaaa gaaaagcact aagtcttcac aatgaaggct ataaggtgga taagaaagta
    12481 tttaaattat ttaattacac aattatgttt gaaaatgcta agtatttaaa agagggaata
    12541 catttaaatc cccaaacaaa aatcaaatta attttaagtg gatatgatga tatattgaat
    12601 aatattataa aaggttttat taaatgtaaa gtatttaaat tgaataatat agagtttaaa
    12661 gtttcagata tagaagaaga ttcaaagaaa aattttaata atataacact ttataaagta
    12721 aggtcaccta ttgtagcaag tttatatgat ttaaaaagta ggaaacaagt atacttaaat
    12781 ccaatgcaag aagagtttta taaagcatta catgataatt taggaaataa gtataagctt
    12841 atacataata aagaatatac tggtgaacta tactttgata ttgaggatgt attagcagta
    12901 aaaaagaaat atataactaa tataaaagga aaaggattta taattggata tacagatttt
    12961 gaaatatttg tacaagctaa taaagatatg caagaagtaa tttattattg tggattaggt
    13021 gaaaaaaata gtataggaat ggggttacta acttatataa cttctaggag ggcttaatat
    13081 ggatagaagt tacaatgtat ttgttgataa tggtctatat gtgttggctt attatctgaa
    13141 caaagatatt aatgatataa cttatcaaga tatagagaat agtatagatt taatgagtga
    13201 taaaattgaa gaatttgtaa gttgcgaaaa atactctaat ttaaaaagta tgtgctttag
    13261 caattctacg ctaactcaac caaaaggaaa agttacttta aatgagaagt taaaaggttt
    13321 tgttaaaaat caaggagatg aatattgtag tttatgtggt caatataagg ccaaagtcaa
    13381 aattgaagat aaagaataca atatagggag aagttatatg cctaatttgg ttgcaaatac
    13441 attttataat ttctccaata atttgcaagg tttgaatgta tgtccatatt gtttagtatt
    13501 gactatgtat tcaattctga attgtagagt aagtaggtat gcttttttat acaattctac
    13561 atcaagcgaa tttatggaag attatacttt ccatattcaa aaagaaaatt taactgatgt
    13621 agagttagga gcaaagaaag aaaaagaaaa gtattcaatt gtaaaattat tagaaagtct
    13681 agtgtgtaaa tataacttat ttaatggtga tatagagcaa tatatgttta ataactctgg
    13741 tcaatctcag gatattcagg taaatagaat aaaaaataaa tatgtgaatt tattaataaa
    13801 attacaggaa aaagcactac tatcaaactt tgaaaagtta catttggata ggtatatctt
    13861 gaatggtact ttagaaagca attatctcag agaagtatat agggtaaaaa aggaagaaaa
    13921 aatggatgag aaagaacaaa agaattttga tgaacttgtt atttttttaa acagggaggt
    13981 aaatgtattg aaaaaagtca aaatagatat aataaaaaaa ttaacggata aattaaaaac
    14041 tagtgactta gatatagaca aaatatataa ggagcttaaa atgttaggaa aattttatgc
    14101 atatgaggac tggttagtta acttaaatga aatgtattat gaaaaaactg gagaaaaact
    14161 atttgaagca gaggattata ttgagttgga tgatataaga agctttagac agattaaaaa
    14221 tttaatatta gtaagtttaa tttaataata ttatggaggg ttaaaatgga aaataaaaat
    14281 tcaaatagaa tgttaaatat aggctatgca atagaagttt tatgtgggcg attaaatgga
    14341 agtagtagtg aagtagaaaa gaaaaataat tcatataaga attctactta tactaaaaaa
    14401 atagggagca gaagtagaat ctcaagtcaa tgtcaaaaat acaatcaaaa gctatttatg
    14461 gaacaagttt gtggttatga acaaaatgtt aaaactaaag atgggaaaca agtaaaattg
    14521 gttccaaacc catttaaata tgcgacagat gatatatttg ggtttatgat ggcggaaaaa
    14581 ttatctatag aagaagatgt atacaaagaa ttatcagatg aagaaaaaga aatatataaa
    14641 aaaaataaaa agaaatatga agtaaatata actaaaaaaa gaaagtcaag atggcaaatg
    14701 aactctttaa taaatgtatc aaatagaaaa gttgaatggg agtggaatgt ttgtggaact
    14761 acaggagata gtatgccata tcaagttgag gtatattcag gggtacatac aaatatagca
    14821 aatcttaata taggaaagat aggagaatat gatatatctg atatagcaag tgagcataga
    14881 gattatacaa ctttagaagc taaatcaatt ggagttgaaa agttagataa agaagaaaag
    14941 tttaaaagaa tagaagctct cctaaaatca ctagaatact taagtgtaga aggtaataag
    15001 acaaatcatc taactgatac aaaacccaaa tttgttatat tgggagagta ttcatgggga
    15061 aataatgtat ttcagggtat tatcaaagaa aatggcttag atgtagagat gttaaaagaa
    15121 tctatagaac aaaatgaaga atttagaaaa agcgatatat ggattggaat aaataaattt
    15181 tatgatgata catttaatga gatagaagtt aataatgaaa aggaacaaat aaagaagtat
    15241 ttagaagaag agtttgaaga gtatgatttt atgaaaatat caaatgtaca tcaagctttt
    15301 gaaagttact taaaatattt aaaggagact ttataaagga tatatcatga ataatttata
    15361 tgaatataaa gctaaatcag atgggacaag tatagataag cataatgaag atatgaatta
    15421 cataatgaat caattagtta gcatatataa tatatctaat gatatgaaaa atgatttata
    15481 tgaggcaatt gtatgtcatg atgtagggaa agtagtaaaa agctttcaag ataatattga
    15541 aagtagcaca agaagagtta gacatgagtt attatcagca agtattaagt atttaaatag
    15601 aggtcagaag ttagctatat taacccacca taaagatata aagtttctag agggtcaaat
    15661 aacaaattct atttatgaaa ttgaattaaa ggaattaaag gaactattag atattgaagt
    15721 agaagatata agaccattta taaaggaaat atcaagagca agaaatgatg atactagaaa
    15781 acttgaaaat atattattaa aaggttactt gcaatattgt gaccatatag cttctgcggg
    15841 aattaaaagt atagataaag gatttaattc tattaaagat tttagtatac caaagggtgt
    15901 aagttataat tcaatacaag aaaaagtttt aaatatggat aaaaatgaag atataatgat
    15961 aatggctatg acaggtcttg gaaaaacagc aacatcattg tattggagta acctagttca
    16021 aaatgaatat aaaagtaaga gagtgtatta tatacttcct tatacagcaa gcataaattc
    16081 tttatatgaa aatttatata atagaaatat aagcgtaagt atgttacaca gtaaagctcc
    16141 atattttttg aataaaatta gtgatgaaga agatataaaa caactttacc agatatacaa
    16201 aaagagtgta aaacaaatta atgtatgtac tatatttcaa ttagtaaagt cagtattttc
    16261 ttgtaagaga tttgaaatgc tactatcaca attaaaagat agtatattta tagttgatga
    16321 aatacattgt tttgatacaa gagtattgtg ttatacattg gaatttttaa aatatttaaa
    16381 agaaaactta aatgtaaaaa tatgtataat gagtgcaagt ataccaactt gcttaaaaaa
    16441 gttaatacag gaaagattgc aaataagtac aattattgaa ccagatagta aagatttaat
    16501 atttagacat agattgaata gattaagtga aggaatactt gatagcatac ataaaataga
    16561 aaatgattta atgcaaggaa aaagagtatt aatatgtgtt aattcagtag atttatctca
    16621 agagttatat ggaaggcttc aaaaatataa tcctaaatta atacatggtc gatttaatac
    16681 tagagataga gagcaggcag aaaaagggat agaagaaagc ttcttattga taggtactca
    16741 aagtatagag gtaagtttag atataagtta tgatgttctt tatacagagt tagctccttt
    16801 agattcattg ttacaaaggt ttggtagagt aaatagaagg ggagataatg ctgagttgag
    16861 caatatttat atatatgatt atgatagaaa gcttaatata tatgatgaag atataataaa
    16921 taggactgat atagtaatat cagatataat acataatgat aattctatag ttgatgagag
    16981 taaagttcca tcttatttag ataatgtcta tttagatata gatatggata aatataataa
    17041 acataaagtt gctttagaga ttataatgaa aaatatgagg ttaggggtat ggaataaatc
    17101 tgctaataat gatatgttaa ataatgatac tattagtgtt gtgcctgaat gtcttttaga
    17161 agagtacaag ttgtatattg ataaaaaaat gtatttagaa gcgagttcat tatttgtaaa
    17221 tgtatctgta aaaagataca attataataa aaagatgttt tatgaatgtg gagatatagt
    17281 tgttacaagt tatagttata atgataaact aggtctaata tttgattgtt aagagaatta
    17341 tagtataact aattaaaata ttttattgag aatttgttat aatttatatc gactacaaat
    17401 tttatatgtt acaatagaat aattttgcag tgagcgaaaa atatgtgtaa atggctgtga
    17461 gttagtgatg gcaaggttta taactgaatt tttgttttta gctaaaaaca ctactggtgc
    17521 ctcactgcaa attaactatt ttaataaccc tacaagtgtt gtaattacta tagttttatt
    17581 tggggtttaa attacactaa gttagttata aatattccgg aaagtacttt tgctatttga
    17641 ttcattctac ccgtttaaat tacactaagt tagttataaa tttcactaat tttagttaca
    17701 actattacaa gtccagccgt ttaaattaca ctaagttagt tataaattat gtgtaatgta
    17761 ttttaatgtt ttctagcaac aatgtttaaa ttacactaaa ttagttataa attttataaa
    17821 ttctatgaat aattttaaag agaggtttcg tttaaattac actaagttag ttataaattg
    17881 tacatttgac atagaagctt caaaatccat cccagtttaa attacactaa gttagttata
    17941 aatgtaacag cacgaatgat ggcacagacc tatctgcaat gtttaaatta cactaagtta
    18001 gttataaata gaagtacact tacaagtaag ataaaagacc caggtacagt ttaaattaca
    18061 ctaagttagt tataaattct gtagctatat gtggaagtaa ttttagtaga gaggtttaaa
    18121 ttacactaag ttagttataa attattgaag gggtgagagt tgtgactagg cactacaagt
    18181 ttagattaca ctaaattagt tgtaaattta cttattcatt aagaaactta actgagagtt
    18241 tgatttatat tattgaatta gcaagagttt tagaaagtag aaaattatac cccaataata
    18301 ccccaaccac aaaaataaat aataaaaaat atccaaaaaa attagtataa catcataact
    18361 aaatagctat atacctacac ttacgaaaac caacaaaaca atcaaataac cacttgtaga
    18421 ctcccctcgt ctccaccaat tttgaaaatg aaaataatag aataaaagga attacttatg
    18481 tttggactaa gtaattcctt ttattttgct taaaagcaca caattagcac acaactagaa
    18541 ggttatataa ataaaaaggc tgtcgcacta gaaaatagtg ctacgctctt ttttgtataa
    18601 aaaaataaat cccaagttca aaagaacctg ggattcttgt ataataactg tatgctaaaa
    18661 caaaatacta cacaaattga ttatactact tatggtgata attatcaatt taagctacca
    18721 ctaaatattg attacatgat tccaaaagat gattctgtaa ggttattaag ttaaattatt
    18781 gaggagatga atttagaaaa attatataag acttattctc gaattagatg aaatagagta
    18841 atccatagac aatttttgaa agttataata tatacagata tgaactgtat ttattcttcg
    18901 agagaaatag aaagaacatg caaaagagat attaatttta tgtaccttct aggcgaaacc
    18961 tccactccag accattcaac aattgcaaaa tttcgcagta tacattttta actaataggc
    19021 aaaagcttga ttgcacagtt tacaaacttt cttggagata ataatgaaat ttcaaaagac
    19081 acaatattta ttgatggtac aaaaatagag tcatctgcta atagatatac ttttgtttgg
    19141 aaaaaagctg taaataaaaa tatggctaaa atggtgaata atatatctga cttcatattg
    19201 aaatgtgaga ttgatgttgg cattaaaata gtttatgaag ataaaattaa aatctatcat
    19261 ttaaagaaac taaaaaaaat atcaaaagaa gcaaatgtag catttattca tggaaaagga
    19321 aatagaaaat ctactaaaaa ccttcatata atgggtgaac gaaatagttg ttctaaaaca
    19381 gataacgatg aaacttttat gagaatgaaa gaagatcata tgaaaaatgg gcaactgaaa
    19441 ccagcatata atatacaatt tggtgtagat taagaatata tagtttggct aaattcagga
    19501 aatcaaactg ctgatactac aacattaata ccatttttaa aatctataga ggaatatact
    19561 aagtttaaat atcctaaagt aatagctgat ggtggatatg aaagtgaata aaactatgca
    19621 tttttagagc ataataatca gttatctttc ataaaacctg caaattatga aattattaaa
    19681 actagaaaat ataaaactta tattggatgt attgacaata tggattatga agaagaaaat
    19741 aattattata tttgtacaaa tgagaaaaaa ctatttgcta ataaaataat aaataggaaa
    19801 tcaaaaacag gatataaaag tgaaataaca tgttacattt gtgaagagtg tagtaattgt
    19861 caatataaaa gcaattgtat taagagaagt aatagtaagg ttcctcttaa aaataggact
    19921 aaaaacttac aagtatctaa gtcgtttcat gaaaaaagaa aagaaaactt aaagagaatt
    19981 atgagcctcg agggggatga acttagagtg aatagaagta tacaagcaga aggagctttc
    20041 gctcaagtaa aataaaatat gaaacttaca agatttttaa gccaaggtaa agaaaatgtt
    20101 ttataaggat atattattgt ggcgatagct tataacgttt ctagactaca caataaaata
    20161 gtaaaaaata aacgtggaat atatctttat catttaaaaa aatattctaa aattattatt
    20221 tataatcaaa tattatctga aaaaatggta tttttatatc tttatttgtc atgcataatt
    20281 ttacttagaa cagaaacttt atataaaaca attgaaaata tacctattaa gataattaga
    20341 aaagagctct cacagaacaa atttaatttg ctcatgcaac agccccatat tgatatacat
    20401 ttaggttgta taattataca tttcaatatt acatgttcat taggataata gttagtaaat
    20461 gatatgattg agagaataga tataataaac taatctagtc ctgtattgca atgtttatta
    20521 aagaatatat ttttaaagat ccaagtactg catctcttcc agaattgagt tggttaatag
    20581 ttttttgatg ctatgcaatt tataataaaa agtataatga tttagttgat gaatcatcag
    20641 aatttatacc ctcaaagatt aatcagattg caataaaaat aatgatatat tctatagtta
    20701 taatatctat tttctttgca acccctcata caatgaatat aaatatatca aacttggata
    20761 tgggtataat tctcatttca gttttattta tacagttttt atctaaatta attttgtatg
    20821 tatattttga taaaagggag atatgtaact aagtggtaat attggtgaaa acaaaaatta
    20881 aagaatatag aaaaaaactc cttatgacac aaaatgaact tgctaaatca gttagggtaa
    20941 ggtgtgagac tattgtacac ttggaaaata taatccttct ttaaagttag tgatagatat
    21001 tgctaaagta ttcaatacta cagtagaaaa tttgtctgaa tttattgaag aaggatagtg
    21061 gatagattat aaatagttat
//
LOCUS       DAEPLF010000045        21005 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEPLF010000045 DAEPLF010000000
VERSION     DAEPLF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21005
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.045"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(29..1420)
                     /locus_tag="KR314_001488"
     CDS             complement(29..1420)
                     /locus_tag="KR314_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBH0327878.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(1431..2651)
                     /locus_tag="KR314_001489"
     CDS             complement(1431..2651)
                     /locus_tag="KR314_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBH0327879.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2665..3453)
                     /locus_tag="KR314_001490"
     CDS             complement(2665..3453)
                     /locus_tag="KR314_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH0327880.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3470..4744)
                     /gene="larA"
                     /locus_tag="KR314_001491"
     CDS             complement(3470..4744)
                     /gene="larA"
                     /locus_tag="KR314_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBH0327881.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(4862..5575)
                     /locus_tag="KR314_001492"
     CDS             complement(4862..5575)
                     /locus_tag="KR314_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBH0327882.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5700..6959
                     /locus_tag="KR314_001493"
     CDS             5700..6959
                     /locus_tag="KR314_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBH0327883.1"
                     /translation="MKKNKIIAFFAPAIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKHEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFIKKFSRKK"
     gene            7227..7793
                     /locus_tag="KR314_001494"
     CDS             7227..7793
                     /locus_tag="KR314_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0327884.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(7994..9199)
                     /locus_tag="KR314_001495"
     CDS             complement(7994..9199)
                     /locus_tag="KR314_001495"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBH0327885.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9302..9408)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9500..9868)
                     /locus_tag="KR314_001496"
     CDS             complement(9500..9868)
                     /locus_tag="KR314_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBH0327886.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(9920..11197)
                     /gene="obgE"
                     /locus_tag="KR314_001497"
     CDS             complement(9920..11197)
                     /gene="obgE"
                     /locus_tag="KR314_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBH0327887.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11308..11598)
                     /gene="rpmA"
                     /locus_tag="KR314_001498"
     CDS             complement(11308..11598)
                     /gene="rpmA"
                     /locus_tag="KR314_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBH0327888.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11612..11929)
                     /locus_tag="KR314_001499"
     CDS             complement(11612..11929)
                     /locus_tag="KR314_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBH0327889.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(11944..12255)
                     /gene="rplU"
                     /locus_tag="KR314_001500"
     CDS             complement(11944..12255)
                     /gene="rplU"
                     /locus_tag="KR314_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBH0327890.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12356..13777)
                     /locus_tag="KR314_001501"
     CDS             complement(12356..13777)
                     /locus_tag="KR314_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBH0327891.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLIIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(13795..14496)
                     /locus_tag="KR314_001502"
     CDS             complement(13795..14496)
                     /locus_tag="KR314_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBH0327892.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14498..16342)
                     /locus_tag="KR314_001503"
     CDS             complement(14498..16342)
                     /locus_tag="KR314_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBH0327893.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFI
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            16661..17854
                     /locus_tag="KR314_001504"
     CDS             16661..17854
                     /locus_tag="KR314_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBH0327894.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(18066..19148)
                     /locus_tag="KR314_001505"
     CDS             complement(18066..19148)
                     /locus_tag="KR314_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBH0327895.1"
                     /translation="MKKIRKLATKLLIATMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGESVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQEEAILDEVYGNNLRPKIERYEGVPCSLINSSINTIKFTSKNSNLDPS
                     RHTVSVIGFMNTPYGMVALAPDGVPVEYPFEYDKPFSLDFTGKNLSAAELSKKCKNNN
                     VIIKIEIGEIEPNSGRGQVRYARYVN"
     gene            complement(19712..20503)
                     /locus_tag="KR314_001506"
     CDS             complement(19712..20503)
                     /locus_tag="KR314_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327896.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTNIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(20507..20836)
                     /locus_tag="KR314_001507"
     CDS             complement(20507..20836)
                     /locus_tag="KR314_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0327897.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
BASE COUNT         6613 a         3797 c         2340 g         8255 t
ORIGIN      
        1 ttttgtaaat attattaaat taatatttct aatatatttt ccctggattt aaaatatttt
       61 tagggtcaaa agctaacttt atatttttaa ttaacatcat ataagcatct gaatttgatt
      121 catgtaagta ctctttttta gcatatccta tgccatgttc accagaaact tgaccactaa
      181 gttctctaga ttttttatac atatagtcaa aagtttcttt taatcttatt ttccatgtat
      241 tatcatccat accatctttt aaaatatata tgtgtagatt tccatctcct gcatgaccaa
      301 aactttttat tcttattttt aatttgtctt gaagctcgtg agtgtatctt ataaactctg
      361 ctatcttatc tcttggaaca acgacatcac attcatccat ttgtgtcgta gatgccttta
      421 tagcttctaa aaatgcacct ctggctgacc atatagaatc gtttctttct tgtgtatctg
      481 atataaatac atctaatgca ccattttcta aacataaatt agctactttt tcatattcct
      541 tttctatatc ttcagtgcta tttccatcaa atgtaagtaa aagataagca tctgatgtat
      601 tatctggaaa ctttttacct aaaaattctt ctgctgccaa tattacatct ctttccataa
      661 attctatagc cgttggtatt gattttgatt ttattatttt aggaacagtt tctattgcca
      721 ttgataaatc tggaaatggt ataagcaaac ttatactttt ctttggtaaa ggaagcaatt
      781 ttaatatagc tttagtaact attcctaaag taccttctga acctactaat aaatccttta
      841 tactatatcc agaactattt ttaaccacct ttccacctac atttataatt tctccatttg
      901 gtagtacaac ttcaagtcct cttacataat cccttgtaac gccatatttt actgctctca
      961 taccacctgc attagtgttt atattacctg caattgtagc acttttttca cctgggtcag
     1021 gtgggtaaaa taaatctcta tcttgaacat attgacctat tgtcatcaat aaaacacctg
     1081 gttctactgt caatgttaaa ttttcataat caacttcaag tattttattc attttacata
     1141 gattaatcat tattccacca tttatggcaa cagcagcacc tacaagacca gtaccttgac
     1201 ctcttggtgt gactggtata ttatttttat atgcgtattt cattatttta gatacttgtt
     1261 cagtttcaag tacatttact aaaacttcag ggtacttctc aatccctcca agttcatcat
     1321 gtgaaaaatc ttcatttata tcacttccaa ctaatacatt ttcttctcca caagtatcta
     1381 ttaaaaaatc tatatctttt ttatcgatta atttatacat ttctatcccc ctatttagct
     1441 gctgatgcca tatatttttg attattatct ttgttatttt ctattttttc aatcaattta
     1501 ggaataatat cataaatatc acctattaaa gctaaatgtg ctacatcaag tataggcgct
     1561 ttatcatctt tatttattgc tactatatag tcagcaccct gcatccctgc aacaaattga
     1621 actgctccag atacaccaca agttattatc aatttaggtt ttacagttct accactaaga
     1681 cctatttgtt ttttagcatc tatccaacct gcttctatta caggtcttgt tcctgccact
     1741 tgagcgttta gcttatcagc taatttatat atcatctcca tatcttcttg tttttttatt
     1801 gctctgcttg ccacaactat cacttcagct tcttccagac caacttcttt atttttttct
     1861 tttacactca gtaccttaat tttagatttt agttcttttt tttctacttt acataaagtt
     1921 atctttcctg tagtattctc tattttttct ggtgctgaaa atattttata tcttacagtg
     1981 gcaaattgtg gtctattatt aggtgtgttt atatgagcca ttatatttcc accaaatgct
     2041 ggtcttattt ggtctaaatc agtatttgat tgaatatcaa gtattgtaca atctgctgta
     2101 agacctgttc taaatctagc ggcaagtctt ggagctaatg acctacctaa tgtagttcca
     2161 ccaactaata ctatcgttgg ttttatttta tctataaagt tttctattgc ttttgaatat
     2221 ggttctatcc taaaatcttt aaattcatct tcatcatata caaatacttc atccacccca
     2281 taagaaatca aattactgca cttatctttt atgtcttttc ctacaaatac acaatacaca
     2341 ggttgtttta tttttgatgc catctcacga gctttaccta taagctcgta cgttacagga
     2401 tgtatatttc cattatgatg ttcaacatat actgcaattc ccctccattc atctttatta
     2461 atttgtactt tactactctc tataaattca aatgcgcctt ttggtccact tttgacacac
     2521 aacttgcaca tcttacagcc agcacttatt tctactatac ctgacttttc ttctatagcc
     2581 ccaaaaggac atatttctaa aattctcttt aagtcagtta ttttatcttg atttacaact
     2641 atctttgaca tttattactc ccccttacac caactttaat tcctctaatt tatttacaag
     2701 tgcaaaactt agttcatctg catttcctct tacaatattt ttatctgtat ttggctttgg
     2761 aggaaatatt cttacaactt gagtaggaga accgttaagt ccatagttat tttcatcttt
     2821 atcttcaaag tcatctaagc tatatacagg tatttctcta tctttagtat taagttttaa
     2881 tttaaatgaa ggtagtcttg gttgaaaaat gtctttttct acagttatta aacatggata
     2941 ttcaatctca catacttgta aatcatttgg catatctatt tcaacaacta tactttcatc
     3001 tttaacttca attaattttg taacgttagt aacatgaggt atatctaaaa attcagctac
     3061 ttcaggtccc acctgtgctg tatcaccatc agtagtttgt tttccacaaa ttattaggtc
     3121 tacctttccc atcttcttaa taccttgtga tatggtatag gaagtagcca atacatctgc
     3181 tcctccaaat ctcctatcac ttattaatgc tccttcatca gctcccatca taaaactttc
     3241 ttcaattacc ttctttgcct gaggaggacc catgctaagt acttttagat tcgctttctt
     3301 atcttccttt attctaagag ctgtttctaa agcatatagg tcatagggat tcatttttga
     3361 atctactcca tctcttttta gtactcctgt agtttcatca acttctactg aagtcgttcc
     3421 aggaacttgt ttaatacatg tcaaaatttc cataaagtac actccttttt tatttaacta
     3481 taactgatac tccatcaggt ataactacta ctttgctctc cttaccaact atactattcg
     3541 ccatttctaa agcttcttta aagttaaatg catgtttcat atgcattttt tttatcattt
     3601 caggattaca cttatctgtt accattatca cagtatattt agataatatt ctcgctagta
     3661 tctgaaattc ccattggtct ggaatcgtat ctactctatc gacacttcta attttatcta
     3721 aaagttgttt tggagaatct gcatttgcta cattttcata aaaagattga cctccatgac
     3781 catcattaca tgctgaaatc attattatta ctccaccgtc tttacaagta gcttcagctg
     3841 ctgtcattcc tttaacagat tgataaatat tttggtctag tggatatcca ccatttgtag
     3901 ttacaacaat atcagatttt atagaattta cttttgataa ctctgttaca aatctacatc
     3961 ctttagtatg tgctttttcg acatcaccag caaaggaagc aattattttt ttatctttat
     4021 ctattacaac attcaatatg aatgctaact ttgcaacttt agctgcatat accatatcct
     4081 catgaatagg attattattc aatttcccag ttcttgaatt atcactatct ataaagtcac
     4141 tacagtgatt gtacattata gtctttgcag atgcgattcc tggaagtacg ctttttctgc
     4201 cacctgaaaa acctgcaaag aaatgaggtt ctataaaacc ttctgctatc aaaagttctg
     4261 cctcatatgc tagtttgttt atatataaat ctccacctga aggcaatatc cctactttac
     4321 acatagattt atcatcagta gatatatgca taactatatc ttcattatct actatttctt
     4381 ctcccatttt ataaattagc tcttcccttg tacttggcct atgaaatcca gttgccacta
     4441 taatcttcac atcaatttct ggattaacat tcctaatcct tcttaatata attggcatag
     4501 ttattctact tggtacaggt cttgtatggt cacttgtaat tatgactaca ttctttttcc
     4561 ccttaaccag ttcttccaga gaattagagc ctataggatt attcatagat tcttcaacta
     4621 tctcttcttg agtcttatgt tctatataat tatccgattt tgactccaaa acaccgagaa
     4681 aacgttcatc aggtatttct aaaatcattc cttgttttga atacggtact tttaacttta
     4741 ccatttttaa tctcccatta taataaacta taaaattggt atgtggtcat accagtttca
     4801 catttatatg atagtaccat tatctaccat ttgcaagaat aatttatttt ataacaaact
     4861 tttaattttc attttttcta taatttttac ttataaaatc catatgttcc tttgctttct
     4921 cacatgcaag ttctacatca ttttctacaa ctgcattgta tatagcttca tgctgtttaa
     4981 gcaatatatt ttcattaaaa ggttctgcga ttattttttc tcttgcatct tctataaatc
     5041 catcaaataa ttttgatgct gtcataaaaa gactttgaat taaataattt ttacttaatg
     5101 ttgctagttt actgtgaaat ttttggtcta ataacaccag tactcgatta ttatcttttt
     5161 tattagtttc tactctcatt tcatctatta tatgtttcaa ttctataact tcagaagaag
     5221 ttattctctt tgaagcaagt tttgcaatct caatttctag gattattcta agctctaata
     5281 tatcttcaag tgtgccatta ttaagtttaa aaatcataga taaaggttct attaaagttt
     5341 tttctatatt tgtacatata aaatttcctt caccctgtct gctttctatc actcccatag
     5401 tctctaatac cctaatagct tcccttatgg aagttctact cacattcatt tgttcagaca
     5461 attctctttc tgatagtaat ttatccccct tttttaactc tccatttagt attttatatt
     5521 gtatttgctc aattacttgt tcataaactt ttttactagc tatactatta aacatttttt
     5581 cctccaatat gataatcgtt gttgtaatat atatctatta tatatcttaa accattacat
     5641 tcatatattt ttattacttt taatataata tcttataact caaatttaca ggtgatatta
     5701 tgaaaaaaaa taaaattatt gctttttttg ctccagctat tatagttgga gttctagttt
     5761 tagggattat cctataccca aatgaagcta taaaatctgc aagagaaggc ttctctatat
     5821 ggtctaatgt tttagttcca tcactactac catttataat tggagcaaac ttaattgttg
     5881 atttaaaaat agtggatatt atagggttta tcatcaatcc cataactaga tttgtattta
     5941 atgtttcagg aaagagcgct ttagcttttg ctatatctac agtatctggt tatccagtag
     6001 gagtaagttt agcttctgaa tttaggagta atgggcaaat ttctaagcat gaagcacaaa
     6061 gacttgtgtc tttttgttct acatcaggac ctttattcat aataggttcg gttggtacag
     6121 gtatgttcca aaattcaaac ttaggttatt taatgattct ctgccattat ctaggaacta
     6181 tattggttgg tcttttcttt agaaattatg gtaatgaaaa tcttccaaag acaaaattgg
     6241 atttaaaaac aaatataaaa aatgtaatca acattagaaa ttcaagtggg aatggttttt
     6301 ttgttttatt tggaaatgct gtatttaatg gagtaaatac attacttaca gttggtggat
     6361 ttgtaattgt tttttcagta gtatttaaaa tactatctct ttttaatgta atctcattaa
     6421 tagcatcagt tatatatttg cctctttcat ttttaggtgt tagtaaagaa ctctgtcaag
     6481 catttgtaag tggtctattt gaaattacta taggatgcaa taaagtatct agtataacaa
     6541 gttctccaga aattttaaga gcatctttag ctagcttttt gataggattt agtggattat
     6601 ccatcctagc tcaatgttgt acatttctat caaaaacgga catagattta aaattataca
     6661 tactaagtaa atttgcgcat ggagttttag ctgctatctt tatatttata ctttatccaa
     6721 ttgcaaactc tgctgtgttg gtttctagct ttgctgatac atacaacgtt atatataata
     6781 atttaatctg gttttattat cttagctatt atgatacaat tcttcaaatt gtagtaataa
     6841 tttatctaat ctcagcagta tttattgcaa aaaaacatat gaaaacttta tctacaaatg
     6901 ggaaactaat gcgatataaa aatacttttt ttattaagaa attttcacga aaaaaataaa
     6961 actaataaaa cttaactaaa tacttccatt tttttattat atttgtcata attttaataa
     7021 cctttttaat tagtttaaca gcatagttat ttcatcacaa tttaataatt ttattagaaa
     7081 tatttttcaa aaaattctcc ttaatcacat atatcacact tgtataagtt tactgggaaa
     7141 tttttccatt atataatgtt tcatttatat tatataataa ataccgacct aaacttacca
     7201 aatcaaacta agaggagtgg ttgaaaatgg caacaagacc tatagaaata gacgaatata
     7261 aaaagataat ggaactatta catactggct ttacttatag tgaaaatggc gttgaaaaaa
     7321 gatttagaaa aaatcctaaa gttgcattgg cactaatgtt agaagctaat ttaggtctaa
     7381 gaatatcaga tattttaaga ttaaagatag gtaattttaa aggtaatatg ttggaaataa
     7441 atgaaaaaaa gacgggcaaa ttacagtata gaccagttaa taaaaatata attgagtcca
     7501 taaatgaaca tgcaagaaag tataatctta aatcaaatga ctacttagta aatataaaga
     7561 caaaggctat tcaaaaacag ttgaggataa tttgtaaaca cttaaatctg tataatataa
     7621 gtacacatag ctttagaaag ctctatgcta ctactcaatt tgaaaagagt aacaataatt
     7681 tagagttggt taaagaactt ttaaatcaca gttctgtagc cactacacag cgatatataa
     7741 gagtaactca gcaagctatt aatgaagcta gtgaaaattt ctttattggt taattataac
     7801 gtatacattt ttctattatc ttattaattc accagatatt aaaaaagagt gtctcaaaat
     7861 gaacttttta gttcatgaga cactcttttt ttataaaatc aaatatattt ttattatttc
     7921 aatactggaa aaaaggcttg tctctctatt ttgagacacc ctattaaatt ttcataattc
     7981 ttgcataaac atattaatct atcttcaata attttttagc tgattcactg taattatttc
     8041 tttttacaat ttccaaaact ctatcaatct catgttctga ctttgcactt acaaaaatcg
     8101 ccaattcttc atcatctata atatcataac tatctacata tttatctaaa cattctaaaa
     8161 ttccatcttt attattatac ccaccaaatc taactactgc actacactca tcttctataa
     8221 gtccacttaa atccaatttc ccttttgaaa aagactttat atttgaactt ctattatcta
     8281 ttatatctat aatatctgca taaacagcac ttcctgttgc acctcttcca gctcccttac
     8341 ctacaaaaga aagttcacct actgaatcac ctactaaaat aactacgttg aactcattat
     8401 ctatctttga aagcatatta ttattgtcca ctaatgctgg tctaacaaat ccacttactt
     8461 ctcctgattc atacttactt ctagcaacca attttatctt acaatttaat ttttttgcat
     8521 attctatatc tttttcatct attgaagtta ttccttctaa taataaatct ttccaatata
     8581 ctatttttcc ataagataaa gtagacaata tagataactt tctagcacta tcatatccta
     8641 aaacatctgc atctggatta acttctgcaa atcccaactc ttgtgcctgt tttaaagcaa
     8701 catcatatgg taaattttca tcatacatct ttgataatat aaaattacaa gtcccattta
     8761 aaatagcgtt tatgctcttt atattgttgc cttccagtga ttctatcaat ggttttaaaa
     8821 ctggaatacc acctgccaca gatgcttcaa acttgatgct aacattattt aagttagcta
     8881 attctataag gtcactacct ttttcagcca gtaaatcttt attagctgtc actacatgta
     8941 tttttttatt taacgctctt gttacataat caaaagctgg atttaatcct ccaattactt
     9001 caactagtat atcaatatcc ttgtcaaata tatcttctat atcagtagtt atcttatcaa
     9061 aatacttctt atacttgtgt ttttctttat ttttaactaa aattccaaca acttctatat
     9121 ctctttttga agtattattt tctattaaat caattaatcc acttcctact gttccatacc
     9181 ctaatattgc tatcttcact gtaacatacc cccatcattt tgattatatt aaattattta
     9241 tatatataaa tataccaata aaaaaagctt gagtataaac tcaagccata tgacgggttt
     9301 atactcatct ttcagcataa atgctgcagg atttagcacc aatgtataat taaatatact
     9361 ggttgccggg cttcacaggg ccagtccctc tgccactctt gataagattg ctaattaaaa
     9421 tttatgttta atagtataat acaatttctt ccaattgtca ctattttttt aacttatttc
     9481 cctaaaagga tataaactat tatctaacag ctcttttagt tggtttaccc ttttttgtaa
     9541 cattattatt tttagtcttt gctttcgcct gttcatgtcc tggaaaaagt attttaggct
     9601 cttcaatatt cctcttgtaa atagtaaatt taaaaccaat agcctgcaca aattcagctc
     9661 ttaaagcttc acatactgca ttagcagttt ctttagtatc aagacctgca ttttctaata
     9721 tagtaacttt tactaattct ctagttctta acataccatc taattgctct aagaaactct
     9781 ctgtaacacc ttctttacca atttgtgtag taggctttaa agtatttgct aaagatctta
     9841 aatacgccct ttgttttcct tttaacatat tctcacctct ataattctat acttaaatta
     9901 tctataatct atatttaaat taattgtaaa actcaaattc taattcataa attttaacca
     9961 catctccatc ttctattccc atttctctaa gcctatcaaa aacaccttga ctctccatag
    10021 ctttttggaa atattgaagg gattccatat catcaaaatt tacagaatac attattcttc
    10081 taagtgcttt acctgtaact acatacacac catcttcaat atctatactt aactcttctt
    10141 cagtaccaat atcaagttct ggtctataca tttcttcttc tgaaactagc tccacatctt
    10201 ctacttcttt tagcatttta gaaacatagg ctattacttc atctatgcct tgacgagttg
    10261 ctgctgacat tttaaatact gtatatcctc tgccttctaa ttcactctta aacttctcaa
    10321 acttactctc atcttctaat atatcaaatt tatttgcaac tacaacttgt ggcctctttg
    10381 aaagcttttc attgtacaat ttaagctcat catttatctt gtcaaaatct tcaatagggt
    10441 ctcttccctc taatccagat atatctacaa tatgtatcaa tacctttgtt ctctcaacat
    10501 gtcttaagaa ctcatgacct aatcctatgc cttcagaagc accctctata attccaggta
    10561 tatctgcaag tacaaaacta tctccaaact tagtttgaac aacacctaga tttggtgtaa
    10621 gtgttgtaaa atgatagttt gcaattttgg gtttagcttt agttacaact gatagaaatg
    10681 ttgatttacc tacatttgga aatcctaaaa gaccaacatc tgctatcatt ttaagttcaa
    10741 gagttatcca tctttcttct ccatctgtac cagattttgc aaaagctgga gcttgtctta
    10801 ctgcatttgc aaagtgttgg ttacctttac cacctatacc accttttgca actatagctg
    10861 tatctccttc tttttttaaa tctgctaata caagacctgt agcctcatct cttattactg
    10921 tacctgctgg tacttttaat actaaatttt ctccattttt accagctctt tttcctttgc
    10981 ttccgtcgcc accattttga gcttgatatt ttttctggta cttaaagtcc attagagttc
    11041 taagccccaa atcaacttca aatatgatgc ttgctccacg accaccatct ccaccgtctg
    11101 gaccaccagc tggaacgtat ttttctcttc taaatgctac cgaaccattt ccaccatttc
    11161 cagccttaac aaatattctt gccttatcta taaacaaatt cttcacctac ttaattaaaa
    11221 acttttaatt agcattacca aaaataccat tataaattca tcagtattat aagaaaaagg
    11281 agtgcctcaa cactcctttt atgtatatta ctcagctatt gctactggat atatactaac
    11341 tttttttctc ttcttgtctt ttctttcgaa tttaacagta ccatcaacta atgcaaataa
    11401 agtatcatca ctaccttttc caacattagt tcctggatgt attttagttc ctctttgtct
    11461 aactattata cttccagcag ttactaattg accatcgaat ctcttaacac ctaatctttt
    11521 agatatagaa tctctaccat ttttagaaga acctacccct tttttagatg ctaataattg
    11581 taaattcatg tttaacatat ggagtcaccc cctacttttt taaaattttt atattttttg
    11641 ggtattcccg tttaatttct ttaaccgcta attgaaagtg atttagtaat aattgtgcgt
    11701 cttttacatg cgtatctttg actatacact ctatatgacc ttcttctcct actacttttt
    11761 caaattcaat cttttgtaat ttgtctaaag aatttataac tgcaatagca ttagatgtaa
    11821 ctgcagcaca tactatatca tagcctattt cagcaaaatc agcatgtccc tttaaacaaa
    11881 aaccttgcaa caagaaatca tcattataat aatatttaac ttttatcata acttaaccaa
    11941 taactaagcg tttatttttt ctacaactat cttagtgtat gattgacgat gtccttgttt
    12001 tcttctgtaa tctttttttg ctttatactt gaaaactata actttcttag ctttaccttg
    12061 ttcaacaact ttagcttgaa cagatgctcc ttctactact ggagaaccta attttaattc
    12121 tccatcttta gagcaagcta atacttcgtt taaagtaaca acatcacctg cgttagcttc
    12181 taatttttca acgaataata catcaccttc agaaacttta tattgctttc caccagtctt
    12241 tactatagcg tacattaaat acacctcctc ggcctcaaac tcgccagtac aaggtacttc
    12301 atttgaagat ttaaaccttt actgtgcggc attacaatta ttaattataa aatatttaca
    12361 ttttattgtc aactatttta tcgaaaataa cattggtttt attgtgcttt aatgtactat
    12421 ttgcgtttaa agaaatcttt atattatatt tttcaccaat tttatcaatt atatccatat
    12481 aatattcttt tatttgttca aaaataaaat cattaaattc taccattatg tttttgtaaa
    12541 cagtatgttc acttattctc ataatttctt tttcaatact atctagtata taatgtacag
    12601 attttattgt ttgttcacca tcacaacatg gacaagacat taaataatac ttatcaatgg
    12661 attctttttc tctacgccta gcaacttcta ctaaaccaag ttttgtcata cctaatactt
    12721 cagcttttct tttatcttta ttaaattctt cttctaggat gtgtaaaatc tccttttgat
    12781 gcttcttttt atgcatatca ataaaatcga ttattattat cccagctatg tctctaatta
    12841 taagctgtct tacaatttct tttgcagctt caagattagt tttataaacg gtttcatcaa
    12901 gtttaccagt tccaataaac ttacccgtat ttacatctat aaccgtaaga gcttctgttt
    12961 tttcaattat taaataacct ccactcttta accatacttt tttatttaat aatttttcta
    13021 ttttagattc tattctgtat aaatcaaata catctctatt ttcctcaaga cacagtttat
    13081 caacataagt cttatctata cctcttaaaa tagacttaag ttcagagtac ttatcataac
    13141 tattggtaat tatagattca atatcatcat tcacattatc ttttacatat ttagttgcaa
    13201 aatctaaaga cttatataat aatttcggac ctataccaag tttatattct tttaaaatat
    13261 tttcatactt taatttcaac tcttctatat ctttctttat ttcatcatga gtgcaacctt
    13321 gagcctcagt ccttattatt aatcctagat tttccttatt tactgcctta gtaatttttt
    13381 ttaatctaaa tcgttctttt tcatcagtaa ttttatttga tattgtagtt ctatcattgg
    13441 aaggtatata gacaatatac cttccagaaa tactaatctc agtgtttaat tttgcgcctt
    13501 tagttcctat ttcttcttta tttatctgaa ccaaaatatc ctgaccagat tttatagtac
    13561 ttcccttttt taattgtaaa taagcaagtt tcttaaatcc aatatctaca aaacaagctt
    13621 ctattcctgg tataactttt tttacgattc cacgatatat attggataca gtcttcttat
    13681 ttaaattgtc ttcaacaaaa agttctgtta atctttcatc ttcaagtact gctgttttct
    13741 gggaccctat tagggactca ataactatct ttttcacaac ttcacctctc gagcttacat
    13801 aggagtcact aattccccat cttctaatac atataaatct cttctcaata tatcaacatc
    13861 taatgggtca atatcaagtt caaacatctc taatatcttt ggtataagta tattagtatt
    13921 taaattagtt tttgagcctg tagctatagt agcagtaaga gtaatatgtt catcttctaa
    13981 atttaaaaca tcaaaggttc ttatcatagg tcttatatct acttcaacca tcttaccttt
    14041 tttattcttt tttgttatga ttatttcttt attattcata aagtcaatta cttttctttt
    14101 tacaaactct ttcgtcatag gtcttttcaa atctatatta aacaaatatt ctccatagtc
    14161 tatcacagaa gaaagagaag gtgtttgtct atcaatttct tttgctttga taaaatctat
    14221 cccatctgga agttgaattg atactttatt taaaaattcc tctactgata aatcatcttc
    14281 ttctatttca atatctacat actcaccttg actttctgtc cctaaagcca aagcattgcc
    14341 ataactcatt ttagggtgag gattaaatcc ttgagaatgt gataagttta tttcagctct
    14401 tctaaacgct ctttgtaaca atctctgaag gtcaagatgt gatatatata tcatgtcgcc
    14461 ttccttttta aattttactc ttattatctt actcatatta gcaaagcccc cttcctatat
    14521 catgtatatt tattccacaa ccattgcagt tatgtctaca atcagaagtg attttttctt
    14581 ttttagcatt ttcgtcttct ctaattaaga attttttact aatacctaca tctatatggt
    14641 cccaaggaaa tacctcttca tagtttctat ttctatgtgc ataaaaatct atagaaagat
    14701 tattcttttc catagcttct ttccatatat ctaaattaaa atgttctgcc catccatcaa
    14761 atttagcacc tagtctaaat gcatcatata taacttttcc tattcttcta tcgcctctag
    14821 ctactactgc ttccatcaaa ctagtctttg aatcatgata attgtatttt atattgttat
    14881 ttcttaattt atttactaaa tgtctctgct tattaataac ttcttctgta gtatcttgac
    14941 catgccattg gaaaggtgta aatggctttg gtacaaatgt agaagtactc actgtaacac
    15001 taaaacttct ctttagcttt cctccattta cttttctata aatatcaata actttataag
    15061 ctaatttagc tataccatct aaatcttcat aagtctcagt tggtaaacca atcataaaat
    15121 acaacttaac actattccac cccatctcaa aagctctttc tgttgcattt tccaaatctt
    15181 cttctgtaac acctttattt ataacatctc taagtctttg agttcctgct tctggagcaa
    15241 aagtaagtcc agattttctt acttgttgta ttttatcagc aatttccatt gaaaaattat
    15301 ctaatctaag ggatggaagc gaaatcccaa tattattaga tgcatattca tctaccaaat
    15361 aatcagtcaa ctctgataac ttactataat cactcgtact aagtgatgat aatgatattt
    15421 catcatatcc agtattctta actaatttat ctaatatttc tttaagtctt ttaacactct
    15481 tttctcttat tggcctgtat atcatacctg cttgacagaa tctacatcct ctagtacatc
    15541 ctctaaataa ttctagtact attctatcat gaacagtatc tatataaggt actattagtt
    15601 tttctggata atctacagtc tcaacatctt ttattatcct tttagtagga tttttagtaa
    15661 tcccttctct attaggagtt acactttgaa ctgtaccatc ttcattgtac ttgacatcat
    15721 aaaaacttgg tatgtatact ccttctatgc tagatatttt atataaaaaa tcttctctag
    15781 tcgttttatt tttcttccat tctttatact catttactac ttctaaatta acctcttcac
    15841 cttcacctaa tattactaca tctataaaat ctgctaatgg ttcggaatta taagcacaag
    15901 gaccaccaac cattataaat gggtcttcca aagttctttc ttcttttaat attggtatat
    15961 tggctaaatc taatatattt aagatattag tgtatgaaag ttcatattga agtgtaaaag
    16021 tgacaaaatc aaagtttgtt ataggctctc ttgactctaa tgcaaataat ggtatacttt
    16081 tttctctcat tatattttcc atatcaactg caggagaata cactctctca caaaatacat
    16141 cttcatcctt atttattaca tcatataata tatgactccc taagtgactc ataccaactt
    16201 catataaatc tggaaagcaa tgtgcatatc ttatcaattc actattacta gtatctttat
    16261 gaattgaatt tatttcattt ccaagatatc tagcaggctt ttctactttt tttaagattt
    16321 ttttcaagtc tactttattc atttattaaa attcctccaa acaagctctt tatttttatt
    16381 cttatttata agtagtatta tacacgataa aatattttct atcaaaaata taactaaaat
    16441 attgaattta aaatacatat tattcacaaa atagaatagc taaaaatatt taagaatatt
    16501 ttctattaaa tttttattga aatacaattt ttttacaaag tatgatatat tataaacata
    16561 aaactattgt tttacatgac ttatcatgac atcttttgtt tcattcacct caaaattggg
    16621 tatgttactt atataatgat aaattttagg agggttaaaa atgagtaaag tttttgaagt
    16681 aaaaaaagat atctacttta ctggcgtagt agatgaaggt ttaaaagtat tcgacataat
    16741 aatggaaact gagtttggga caacttacaa ctcttattta atcaaggacg aaaaaacggt
    16801 tttatttgac actgtaaagg caaattttaa agatgagttt ttaagtaatt tatcagaggt
    16861 tactgatata gctaaaattg actatgttgt aattcatcac acagaaccag accatgcagg
    16921 tagtttaaaa tacttattag atataaatcc taatatagaa gtttattgta ctaaagctgc
    16981 taaactatac ttagatggtc aaataaatag accttttaac tgtcatgtaa taaaagatgg
    17041 tgaaatttta aatatcggta aaagaaatct tagatttata actgctccat tcttacattg
    17101 ggttgataca atgtttactt acattgagga ggacaagact cttttaactt gtgatgcatt
    17161 tggttgtcac tttgcaagtg ttgatgcaga ggtagtgaat agtgaagatt atctaaaatc
    17221 agctaaacac tactatgatt gtatagttaa gccatttgct aaacatgttt taagtgctgt
    17281 agataaggtg gtaggactaa acatagaatt tgatactata ttgacttctc atggtccaat
    17341 gcttactaaa gacccaatgg ctgctgttaa aagatatgtt gagtggtcta cagaagctgt
    17401 taatacaact aatcaaaatc aagtttctat attctactta tcagcttata gtaatacttt
    17461 agagatggct aaaaagataa aagaaggact tgataaagaa ggtgcaaagg ctgaattata
    17521 tgatttagaa gatatgacat taacagaaat gcacgataca ttagtagtgt caaaagtaat
    17581 attactaggt tctccaacta taaacaaaac tatggttaaa cctatgtggg atttattctc
    17641 tgttatagac ccaatggcta accaaggcaa gatagcagga gtatttggtt catttggttg
    17701 gagtggtgaa ggtataacta tggctgagac tttacttaaa tctatgtcat tcaaaatgcc
    17761 tgtagaatct cttaagaaaa aattcttccc tagtgaagag acattaaaag agtgtatggc
    17821 atttggtgca gaatttgcaa aattagttaa atagccatta atcacacata atattattta
    17881 aaataaatga ttttatattt aaaaataaat taaaatggag ttgtctcaaa attaattttt
    17941 ctaatctaat tttaagttca actccttttt tatttcccaa aatgactaat ataatgataa
    18001 atgcaccctc accaagaggg tacattattt ataatctttt ctatttgtat acttgtatat
    18061 ttatattaat tcacatatct agcatatctt acttgccctc ttccagaatt aggttctatt
    18121 tcaccaattt ctattttaat aattacatta ttgtttttac acttttttga taattctgct
    18181 gcagaaaggt ttttaccagt aaaatctaaa gaaaatggct tatcatattc aaaaggatac
    18241 tcaacaggta caccatctgg agctaatgca accataccat atggtgtatt cataaaccct
    18301 attacagaaa ctgtatgcct tgatgggtct aaatttgaat ttttgcttgt aaacttaatt
    18361 gtatttattg atgagttgat taaactacac ggaactccct catacctttc aattttggga
    18421 cgtagattat ttccataaac ttcatcaagt attgcttctt cttgatctgc tatcatagtg
    18481 tgattcccag gtccaactgg cgcagaaggc ttagctggcg taggtttctt aacagttggc
    18541 gttggattct gtgtctttac tgattcacca acagctggat aggctttctt gtcaaatggt
    18601 aatgggtgag taagactctt gtatatgatg tctgctcctg ctgatttgtt ggcatcaata
    18661 tctacatgat acaaacgtcc atcctcaaaa cgaataagat tccactgatg gtcatttgtt
    18721 gtactatgta cataataaca ttcaattccc atagcatcac ataaagcttt caagctccta
    18781 cacattccag aacaagcacc ttttttagta accaatgtat aagcagtatt ggctccctct
    18841 gctgcgttcc agttaacata tgttgtgttg tttttaatgt gattataagc aaccctaatc
    18901 ttagcatctg tggtcatgtt ctttgtaatt ttcttgttca taaagtctgc tgttgcttgt
    18961 gctaccgaat taagttgagc ttgtgataac tttggaccat atatactgtt ggttccagct
    19021 acatagcttt tcggtgtctt tgcagtaaca tctttagctg tcataccata aactgttaca
    19081 ctcatacatg aggttaaaac catcgttgca atcaatagtt tagtagctaa tttcctgatt
    19141 tttttcatat tagcattctc ctttataata tattttactt tcaattccct accattaatt
    19201 aagttcattt tagatttaaa atatggatta tcaacatgtg tgcatagcaa ttcacgtggt
    19261 catagaatat ttagatttct tggaaaatct gttctatatt tcctatatag ttcgtacaca
    19321 atccaactca caaacctgca tttttgatat catcacaata aaatgataat aattaatata
    19381 ttcaaaaatg ttggtctgta acaggtttgt caattttcta attattggta atgacgagag
    19441 tgaggagaat tgtcaataat acatttaaaa ttaaaattgt atacacttca tacattctcc
    19501 cctaacaaca ttatacagat tataatttgt atattcaata cattttttgg aaggtataat
    19561 tatatcaaaa tatgtgatta tgtatagata caatccacta tggagaaata taaaagaaaa
    19621 gagtataaca aacaataaaa aataagatga ttcaaatttt attttgaaac atcttattta
    19681 ctctacaaat taaaattatt tggtgaatta tttaattatt attttttatt aaagaaattt
    19741 taaatcttaa tataaaatac gctattatac ataataatcc aaatgcaaat gctactggaa
    19801 ttaacatctc cgattcaaaa taaaagaaat attctaagaa aatatctgga tttactatat
    19861 tcccattctt gtctaaaaaa tcttttccat tatatcctaa atgctgtata aaattactta
    19921 tgttattata atctgcttcc ctctcatata ccatattagc attcaatcca gaagaactta
    19981 aattagtgtt tccatctttc atatctatta catatagtct aagagctttg actttactat
    20041 acttatcttt atcaagtatt ttttcaagtt ctaacttata ttcaccaata tttgtcatat
    20101 catgtctttc tgccaattgc tttatttctc catcaagttc ttttcctaaa ttatacatat
    20161 tatgttgtaa actattattg taataccaca taaatccaga aattaaaaat gcaattatac
    20221 ttatgtaacc taatactttt ttaaacccca ttacaattga tttatgccta tccaatgata
    20281 aagatagttc ttttattata ttacataatt catactgcat ttcatcacca tcatcaaatc
    20341 tttcaattgc ttttttacat gcttcttctt catctaatcc ttctaattta aattcatttg
    20401 cagattctat aagatggcat ctcatttcct gttttaattc tatagtactt ttattgtcac
    20461 attttttata tagtttatct aaatattcat ctattagttt cataaattaa tccacctctc
    20521 ccaaaaattg attcataacc tttttaataa atctccattc ttgtttcttt atttctaaaa
    20581 aatctttacc ttcattagtt aaattataat attttcttct tccacctgaa ctttgttcat
    20641 tgctataata agatttaatt aaatttttag attccattct tttaagtgca agatacattg
    20701 ttccttcttt taattcaaac tcacttcttt ctcttacttg ctttgcaatt tcatatccat
    20761 aacaatcttt cttttctaat atagaaacaa ttaatatatc gatataacct tttaacactt
    20821 ctttatttaa ctgcattatg taccaccgcc ttttcaccta ctactctata atgtatagta
    20881 cttatattta ctataataat acatagtact ctataataca aggtttatat tctacaatag
    20941 acttaaaaat aataaatagc tgtatattag tatttataga ttaaatacta atatacagct
    21001 attta
//
LOCUS       DAEPLF010000046        20685 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEPLF010000046 DAEPLF010000000
VERSION     DAEPLF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.046"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            358..669
                     /gene="rpsJ"
                     /locus_tag="KR314_001508"
     CDS             358..669
                     /gene="rpsJ"
                     /locus_tag="KR314_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBH0327898.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            761..1390
                     /gene="rplC"
                     /locus_tag="KR314_001509"
     CDS             761..1390
                     /gene="rplC"
                     /locus_tag="KR314_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBH0327899.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1420..2043
                     /gene="rplD"
                     /locus_tag="KR314_001510"
     CDS             1420..2043
                     /gene="rplD"
                     /locus_tag="KR314_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBH0327900.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2043..2333
                     /gene="rplW"
                     /locus_tag="KR314_001511"
     CDS             2043..2333
                     /gene="rplW"
                     /locus_tag="KR314_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBH0327901.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTASFKKAVVKLTADSKEIEFFQGM"
     gene            2363..3193
                     /gene="rplB"
                     /locus_tag="KR314_001512"
     CDS             2363..3193
                     /gene="rplB"
                     /locus_tag="KR314_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBH0327902.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3228..3509
                     /gene="rpsS"
                     /locus_tag="KR314_001513"
     CDS             3228..3509
                     /gene="rpsS"
                     /locus_tag="KR314_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBH0327903.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3541..3876
                     /gene="rplV"
                     /locus_tag="KR314_001514"
     CDS             3541..3876
                     /gene="rplV"
                     /locus_tag="KR314_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBH0327904.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3899..4714
                     /gene="rpsC"
                     /locus_tag="KR314_001515"
     CDS             3899..4714
                     /gene="rpsC"
                     /locus_tag="KR314_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBH0327905.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4750..5181
                     /gene="rplP"
                     /locus_tag="KR314_001516"
     CDS             4750..5181
                     /gene="rplP"
                     /locus_tag="KR314_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBH0327906.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5183..5386
                     /gene="rpmC"
                     /locus_tag="KR314_001517"
     CDS             5183..5386
                     /gene="rpmC"
                     /locus_tag="KR314_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBH0327907.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5410..5664
                     /gene="rpsQ"
                     /locus_tag="KR314_001518"
     CDS             5410..5664
                     /gene="rpsQ"
                     /locus_tag="KR314_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBH0327908.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5690..6058
                     /gene="rplN"
                     /locus_tag="KR314_001519"
     CDS             5690..6058
                     /gene="rplN"
                     /locus_tag="KR314_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBH0327909.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6082..6387
                     /gene="rplX"
                     /locus_tag="KR314_001520"
     CDS             6082..6387
                     /gene="rplX"
                     /locus_tag="KR314_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBH0327910.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6417..6959
                     /gene="rplE"
                     /locus_tag="KR314_001521"
     CDS             6417..6959
                     /gene="rplE"
                     /locus_tag="KR314_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBH0327911.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6977..7162
                     /locus_tag="KR314_001522"
     CDS             6977..7162
                     /locus_tag="KR314_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBH0327912.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7195..7593
                     /gene="rpsH"
                     /locus_tag="KR314_001523"
     CDS             7195..7593
                     /gene="rpsH"
                     /locus_tag="KR314_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBH0327913.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7624..8166
                     /gene="rplF"
                     /locus_tag="KR314_001524"
     CDS             7624..8166
                     /gene="rplF"
                     /locus_tag="KR314_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBH0327914.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8202..8570
                     /gene="rplR"
                     /locus_tag="KR314_001525"
     CDS             8202..8570
                     /gene="rplR"
                     /locus_tag="KR314_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBH0327915.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8591..9100
                     /gene="rpsE"
                     /locus_tag="KR314_001526"
     CDS             8591..9100
                     /gene="rpsE"
                     /locus_tag="KR314_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBH0327916.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9115..9300
                     /gene="rpmD"
                     /locus_tag="KR314_001527"
     CDS             9115..9300
                     /gene="rpmD"
                     /locus_tag="KR314_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBH0327917.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9333..9776
                     /gene="rplO"
                     /locus_tag="KR314_001528"
     CDS             9333..9776
                     /gene="rplO"
                     /locus_tag="KR314_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBH0327918.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9820..11088
                     /gene="secY"
                     /locus_tag="KR314_001529"
     CDS             9820..11088
                     /gene="secY"
                     /locus_tag="KR314_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBH0327919.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11105..11755
                     /locus_tag="KR314_001530"
     CDS             11105..11755
                     /locus_tag="KR314_001530"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBH0327920.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11756..12502
                     /gene="map"
                     /locus_tag="KR314_001531"
     CDS             11756..12502
                     /gene="map"
                     /locus_tag="KR314_001531"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBH0327921.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12524..12793
                     /locus_tag="KR314_001532"
     CDS             12524..12793
                     /locus_tag="KR314_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBH0327922.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12831..13049
                     /gene="infA"
                     /locus_tag="KR314_001533"
     CDS             12831..13049
                     /gene="infA"
                     /locus_tag="KR314_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBH0327923.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13085..13198
                     /gene="rpmJ"
                     /locus_tag="KR314_001534"
     CDS             13085..13198
                     /gene="rpmJ"
                     /locus_tag="KR314_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBH0327924.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13351..13722
                     /gene="rpsM"
                     /locus_tag="KR314_001535"
     CDS             13351..13722
                     /gene="rpsM"
                     /locus_tag="KR314_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBH0327925.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13758..14156
                     /gene="rpsK"
                     /locus_tag="KR314_001536"
     CDS             13758..14156
                     /gene="rpsK"
                     /locus_tag="KR314_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBH0327926.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14188..14811
                     /gene="rpsD"
                     /locus_tag="KR314_001537"
     CDS             14188..14811
                     /gene="rpsD"
                     /locus_tag="KR314_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBH0327927.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14889..15836
                     /locus_tag="KR314_001538"
     CDS             14889..15836
                     /locus_tag="KR314_001538"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBH0327928.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15857..16198
                     /gene="rplQ"
                     /locus_tag="KR314_001539"
     CDS             15857..16198
                     /gene="rplQ"
                     /locus_tag="KR314_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBH0327929.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16318..17151
                     /locus_tag="KR314_001540"
     CDS             16318..17151
                     /locus_tag="KR314_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBH0327930.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17139..18005
                     /locus_tag="KR314_001541"
     CDS             17139..18005
                     /locus_tag="KR314_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBH0327931.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDISFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            17999..18802
                     /locus_tag="KR314_001542"
     CDS             17999..18802
                     /locus_tag="KR314_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBH0327932.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18834..19565
                     /gene="truA"
                     /locus_tag="KR314_001543"
     CDS             18834..19565
                     /gene="truA"
                     /locus_tag="KR314_001543"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBH0327933.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19685..20116
                     /gene="rplM"
                     /locus_tag="KR314_001544"
     CDS             19685..20116
                     /gene="rplM"
                     /locus_tag="KR314_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBH0327934.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20145..20537
                     /gene="rpsI"
                     /locus_tag="KR314_001545"
     CDS             20145..20537
                     /gene="rpsI"
                     /locus_tag="KR314_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBH0327935.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8261 a         2440 c         4323 g         5661 t
ORIGIN      
        1 atggatttaa tataaaaaaa agaggggggt aactcctctc ttttttgttt tttgttgaca
       61 agtagcgcta aagcatatat actaaactag tgccttgaga cagattaaca atcaagttta
      121 aggttatgat gttgtaatag aaaataaaat tgaaaaaaac agaaaaaaaa cttgaaaaaa
      181 tgtaacagat gatataaaat ataatagtgt gcttgagaga ttaagaaaat aataattaaa
      241 tcctagtgaa atacgaactg atatcgattt aaaagttaca acacacccgg agcagtgtat
      301 cggtattggt tgtacgcaac ttgaaaatta aacacgtgat aaaggaggga aaacaaaatg
      361 gctaagaatg aaaaaataag aataagatta aaatcatatg atcacaaatt attagatttt
      421 tcagcaggta aaatagttga aactgctaaa aaggctggat cacaagtttc aggacctgtg
      481 cctctaccaa cagaaaaaca agttgtaact atattaagag ctgtacataa gtacaaagac
      541 tctagagaac aatttgaaat aagaactcat aaaagactaa tcgacatagc taatccaaca
      601 cctaagacag ttgactcatt aatgagatta gacttaccag caggtgtaga tatagaaata
      661 aaattataag aacccctaag atattatcct atagatgatt atcttagaat gattactcta
      721 aaagatgtaa tccgctgtaa gaattatagg aggtgctaat atgaaaggaa tattaggcaa
      781 aaaagttggt atgactcaaa tattcactga taaaggtgta gttatacctg taacagctgt
      841 tgaagcaggg cctatggtcg ttactcaaat aaaaacagtt gataaagatg gatacaatgc
      901 tatacaaata ggttttgaag atgctaaaga aaaagcttta aacaaaccta aaaaaggaca
      961 tttagctgct gctaatgttt taaagaaaca tttaaaagaa tttagagtag attctgtaga
     1021 aggatacaca gtaggacaag aaataaaagc tgatgtattt gaagcaggtg caaaaataga
     1081 tgttactgga ataagtaaag gtaagggatt ccaaggtcca ataaaaagac atggacaaag
     1141 tagaggtcct gaaactcatg gttctagata ccacagaaga ccaggttcaa tgggagcttg
     1201 ttcttaccca ggtagagtat ttaaaaacaa aaaattagct ggacatatgg gaagcgtaaa
     1261 ggtaacagtt caaaacttag aggttgttaa agtagatgct gacaagaacc ttatattagt
     1321 taaaggagct ataccaggag ctaaaggttc agtagtaact ataaaagaag ctataaaggt
     1381 ttctaaataa tgactaacct agagaaagga ggaataacaa tgccaaaatt aaatgtacta
     1441 aatgttagtg gacaaaacgt tggagaaata gaattatcag attctatatt tggcgtagaa
     1501 gttaatggac atgttttata cgaagttgta aaaaatcaat tagcaaataa gagacaagga
     1561 actcaatctg ctaaaactag agcagaagtt agaggtggcg gaagaaaacc ttggaaacaa
     1621 aaaggaacag gtagagcaag acaaggttct acaagatctg tacaatgggt aggtggagga
     1681 gttgcttttg cacctaaacc aagaagctac aaatatacat tacctaagaa ggtaagaaga
     1741 ttagctatga agagtgcttt atcttcaaaa gttcaaaata gtgaagttat agtattagat
     1801 gcattaaaca tggatgctcc taagactaaa gaatttgctc aaatattaaa caatataaat
     1861 gctgctaaaa aagctttagt agttatagct gacaaaaacg ataacgtaat aaaatcagct
     1921 agaaatatag aaggtgttca aactgcatta gttaatacta tgaatgtata tgatatatta
     1981 aaatacgatt catttataat aactacagat gctgttaaaa aagtggagga ggtgtacgca
     2041 taatgactaa tccacatgat gtaataataa gaccagttgt aactgagcac agtatggctg
     2101 aaatgggtga aaaaaaatac acttttgttg tggctaaaga tgcgaacaaa actgaaataa
     2161 aaaaagcagt agaaaaagta ttcggagtta gtgttgataa agtaaatact ttaaactacg
     2221 atggaaaagt taaaagaatg ggtagaactc aaggaagaac agcaagcttt aagaaagcag
     2281 tagttaaatt aactgctgat agtaaagaaa tagaattctt ccaaggaatg tagtattata
     2341 acaaacgaag gagggaaaac agatggctat aaaaaagttt agaccaactt ctcctgcctt
     2401 aagacaaatg acagttttag tttctgatga aataacatgt aatcaaccag aaaaatctct
     2461 tttagttaac ttaaagaaaa atgctggtag aaacgtacat ggtagaataa ctgttcgtca
     2521 tagaggtgga ggacaaaaaa gaaaatatag aataatagat tttaaaagag ataaagatgg
     2581 tatacctgct aaggttgcta ctatagaata tgatccaaac agaactgcaa atatcgcatt
     2641 attaaactat gctgatggag aaaaaagata tatattagct ccagttggaa ttaacgttgg
     2701 agatactata ttatcaggat taggtgctga tataaaacca ggaaactgtt tagcattaaa
     2761 agatatgcca gttggtacta tcattcataa tatagaatta aagccaggaa aaggagctca
     2821 attagttaga tctgctggag tttctgcaca attaatggct aaagaaggaa aaaatgcttt
     2881 attaagatta ccatcaggtg agatgagatt agtaagcata aactgtaaag ctactatagg
     2941 tcaagttgga aatatagagc atggtaacgt agttatcggt aaagcaggta gaaaaagaca
     3001 tatgggaata agacctactg taagaggttc tgtaatgaac cctaatgacc atccacacgg
     3061 tggaggggaa ggtagatctc caataggtag accatctcct gttactccat ggggtaaacc
     3121 agctcttgga tacaaaacta gaaagaaaaa taaagcttca aataaactaa tagtatcaag
     3181 aagaactaag tagtaaattt atttttgctc ggaaaggagg aatataaatg tcaagatcaa
     3241 ctaaaaaagg gccttttgtc catgcaagac ttttaaagaa gatagaagct atgaatgcta
     3301 gtggaaataa agaagttata aaaacttggt caagatcttc aactgtattt ccacaaatgg
     3361 tagaaaacac tatagctgtt catgatggaa gaaaacatgt tccagtatat ataacagaag
     3421 atatggttgg acataaatta ggtgagttcg ttcctactag aactttcaaa ggacataagg
     3481 atgacgaaaa atctaataag agaaaataat taaattatga ctaagaaagg aggaataaca
     3541 atggaagcaa aagctactgc taaatacgta cgtgtatcac caagaaaagc aggccaaata
     3601 tgtgaccttg ttagaggaaa gaatgttgat gaagcattag caatattaaa gtttactcca
     3661 agaggagcag cttcaataat agctaaggtt gtaaaatcag ctaaagctaa cgcagaaaac
     3721 aatcacgaaa tggatactga aaaattatat atagcatcaa tagttgctaa tcaaggacca
     3781 acaatgaaga gattcatgcc tagagctatg ggtcgtgcaa ctacaataag aaaaagaact
     3841 tctcatatag aggttgttgt taaagaaaaa aaataattaa tagataggag ggaaagtaat
     3901 gggtcaaaag gttaatccac acggcttaag ggtcggtgtt ataaaagatt gggactcaag
     3961 atggttcgct actgataaaa aagagttcgg aaacttatta ttagaagacc ataatatacg
     4021 taaattctta aagaaaagat tatactcagc tggagttgct aagatagaaa tagaaagatc
     4081 agctaacaaa ataaaaatgg acttacacgt tgctaagcct ggtgtagtta taggaagagc
     4141 tggtgcagga atcgaagcat taaaagctga attagaaaaa atgacaaaga aaactataat
     4201 agttaatata gtagaagtaa gaagtacaga taaaaatgct caattagtag ctgaaaatat
     4261 agctttagct atagagagaa gggttgcttt cagaagagca atgaaacaag ctatacaaag
     4321 agcaatgaaa tctggtgcta aagggataaa agtttctgct tcaggaagat taggtggagc
     4381 tgaaatggct agaactgaag gttatagtga aggaaatgtg ccactacaaa ctttaagagc
     4441 agatatagat tatggtttcg ctgaagctga tacaacttat ggaaaaatcg gtataaaagt
     4501 ttggatatgt aatggtgaag ttttaccaac tagagacggt gtaaatccaa gagaagaaag
     4561 cagaaagagc gatagaagag acaacaagag agataataga agaaacgata gaagaggaaa
     4621 tgacagaaga ggaaatgata acagaggaaa ctacagagga caaagacctc aaggtggatc
     4681 aagacctcaa agaactgaaa acaaaggaaa ttaataatag ttaggttcaa ggaaggagga
     4741 aaaatactca tgttaatgcc aaaaagagta aagcgtcgta gagttcatag aggaagcatg
     4801 gctgggcaag ctcataaggg taataaagtt acttatggtg agtttggatt agtagcatta
     4861 gaagcttctt ggataacttc taatcaaata gaagctgcca gaatcgcgat gactagatat
     4921 ataaaaagag ggggaaaggt ttggataaaa atattccctc ataaaccagt aacaagaaaa
     4981 ccagcagaga ctcgtatggg tgcagggaaa ggttctccag aatattgggt agctgtagtt
     5041 aagccaggaa gagttatgtt tgaattagca ggtgtttctg aagataaagc aagagaagct
     5101 atgagacttg ctgcacataa attaccaatc aaatgtaaat ttgttaaaaa agaagattta
     5161 gaagtaaagg gtggtgaata ggatgaaagc taaagaatta agagatttaa caagcgaaga
     5221 gctaatgaac aaattaaacg acttcaaaag tgaattattt agcttaagat tccaattagc
     5281 tactggtcaa ttagaaaata cagctagaat aaagtttgtt aagaaggata tagcaaaagt
     5341 taaaactgtt cttgctgaaa gaaagttata cgaaactaga gcttaatttg gaaaggaggc
     5401 tttttaaaca tggaaagagg aagaagaaaa gttagaatag gccgtgttgt tagtgataag
     5461 atggataaaa caatagtagt agctgttgaa gaattcgtac gtcatccact atataataaa
     5521 cgtgttaaga gaactaagaa attcaaggct catgatgaaa agaatatatg taacatcgga
     5581 gatagagtta aaataatgga aactagacca ttatctaaag ataagagatt cagactagtt
     5641 gaagttgttg agaaagttaa gtagtttttg aagaaggagg gattacgata tgatacaaca
     5701 agaatcacgt ctaagagttg ctgataactc aggagctaaa gaacttttat gtatacgtgt
     5761 tctaggcgga agtaaaagaa gatatggtaa cataggcgac gttatagttg ctactgttaa
     5821 aagtgcaaca cctggtggag ttgtaaaaaa aggtaaggtt gttaaagctg ttatagtaag
     5881 aagcaaacaa ggcgtaagac gtaatgacgg aagctatata tcttttgatg aaaatgctgc
     5941 tgttataata aaggacgata aaactccagt aggaactcgt atattcgggc ctgttgctag
     6001 agagttaaga gacaatgaat tcatgaaaat agtttctctt gctccagaag tactataatt
     6061 aaggaggtgc aataggacat gatgcgtgtt aaaaaaggtg acactgttgt agttatagca
     6121 ggtaaagata aaggtaaaaa aggttcagtt ttaaaggtat atcctaaaac tagcaaagtg
     6181 ttagttgaag gtgtaaatgt aataactaaa catcaaaaac caagtgctat gaaccaacaa
     6241 ggtggaataa taaataaaga agccccaata cacatatcta atgtaatgcc atttgatcct
     6301 gaaacaggaa aaggcgttag agtaagatat gaagtaaaag atgggaacaa agtaagagta
     6361 tcagcaaaga gcggaaaaga attataataa atattgcttg aaaggaggga ccttaaatgg
     6421 cttctagatt acaagaaaaa tacatgaaag aagttgctcc tgctttaatg gagaaatttg
     6481 gatacaaaaa cgtaatggag atacctaagt taaataaaat agttattaac atgggtatag
     6541 gtgacgctag agaaaatcca aaaggattag aaaaaggcgt tgaagaatta gaaatgatat
     6601 caggacaaaa gcctgttata actaaagcta gaaaatctgt tgctaacttc aaattaagag
     6661 aaggaatgcc aataggaaca aaagttacat taagagctga caaaatgttc tactttatgg
     6721 acaaattagt ttcagtttct ttaccaagag ttagagattt tagaggagtt aaccctaatg
     6781 cttttgatgg tagaggaaat tatgctttag gagttaaaga acaattaata ttccctgaaa
     6841 tagaatatga taaaatagat aaagtaagag gaatggatat aatatttgtt actacagcaa
     6901 aaactgacga agaagctcgt gaattattaa aattattagg aatgccattt tctaagtaag
     6961 caaaggaggg attcccgtgg ctagaaaagc gatggttgtt aagcaacaaa gaaagcaaaa
     7021 gtacgctact agagaataca ctagatgtac tatatgcgga agaccgcatt cagttttgaa
     7081 aaaatttggt atatgccgta tatgctttag agaattagct tataaaggtc aaatacctgg
     7141 tgttagaaaa gcaagctggt agaagcttga ttagaatgga aggagggcaa caaaatgaca
     7201 atgacagatc caattgcaga tatgcttact cgtataagaa atgctaatgt tgttaagcat
     7261 gaaactgtag atgttccagc ttctaatatg aagaaagaat tagctagaat cttattagaa
     7321 gaaggtttca taagaggtta tgatgttata gaagatggaa aacaaggtat cataagaata
     7381 caattaaagt acggacaaga aggcgaaaga gttataacag gtcttaagaa aatatctaag
     7441 cctggaatga gagtttacgc tgctaaccat gagataccaa aagtattaaa cggattagga
     7501 atatcagtta tatctacttc aaaaggaata ttaactgaca agcaagctag aaaagaaaat
     7561 gttggtggag aagtaatctg ctacgtttgg taattaatat gaacaatgta aggaggtgcg
     7621 accatgtcaa gaataggtgt taagccaata ataataccag caggtgttga agttactata
     7681 gctgaaggaa atttagttac agtaaaaggt ccaaaaggaa ctttaactaa acaattaagt
     7741 gctgagttaa acataaaaaa agaagaaaat acaataatgg ttgaaagacc aactgataat
     7801 aaaaaacata gatctttaca cggactaact agaactttat tagataatat ggttgtaggt
     7861 gtaaatactg gtttcgaaaa gaaattagaa ttaaaaggtg ttggatatag agctcaaaaa
     7921 caaggaaaga aattagttat gaacttaggt ttctctcatc cagttgagat ggaagatcca
     7981 gaaggaataa ctgttgaagc tccaaaccaa acagagttaa tagtaaaagg tatagacaag
     8041 caattagtag ggaattatgc tgctaagata agagcttgga gaaagccaga gccatacaaa
     8101 ggtaaaggta taaaatacgt tgatgaagta atcagacgta aagaaggaaa aactggtaaa
     8161 aaataatacc aaaacttaaa cgctagaaag gagtgagcta ggtgttaaaa aaggctgata
     8221 aaaatgctaa tagacttcaa agacataaga gagttcgtag aaaaataagc ggtacttctc
     8281 aaagaccaag attatgtgta tttagaagtg ctaacaatat atatgctcaa ataatagatg
     8341 acacaaaaag agtaactcta gttgctgcat cttctttaga agcagaagtt aagagtgctg
     8401 tcaatcatac aggaaacaag gaagcagcta agaaagttgg agaattagtt gctaaaaaag
     8461 ctgttgaaaa aggaatcact gaagttgtat ttgacagagg tggatactta tatcatggaa
     8521 gaattcaaga attagctgaa ggtgctagag aagctggtct taagttctaa tacgaaggag
     8581 ggaaaaatac atgctacgtc gtaagccgat agatgcagga caacttgact tacaagaaaa
     8641 agttgttgaa gtaagacgtg ttactaaggt tgtaaaaggt ggtagaaatt tcagatttgc
     8701 agcattagta gttgttggag atgaaaacgg acacgttggt ataggtgctg ggaaagcaat
     8761 ggaagtacca gacgctataa aaaaagcagt tgaagatgct aagaaaaatc taatagtagt
     8821 gccaatagtt gggactacaa ttcctcacga agtacgcgga cactttggtg ctggaaatat
     8881 attaataatg cctgctgtag aaggtacagg agtaatagct gggggacctg ctagagccgt
     8941 acttgagcta gctggactaa aagacgttag agctaaatct ttagggtcta acaatccaag
     9001 aaatatggta aatgctacaa tagaaggact taattctcta aaaacagttg aagatatagc
     9061 taaacttaga ggtaaaaaag ttgaagaact tctagggtaa ggaggtagtg taaaatggct
     9121 aaattacaaa taaagttagt tagaagtgtt ataggaacta ctcctaacca aaaaaagaat
     9181 gttgaagcgt taggattaag aaaaagagaa caagttgttg taaaagaaga caatgctcaa
     9241 acaagaggta tgataaataa agtaagtcac ttattagaag taactgaaat agctgaataa
     9301 tttttataaa gtagtgcaag gaggtgcaat ccatgaagtt acatgagtta aaacctgctg
     9361 aaggtgcagt aagagctaag agaagattag gtagaggtac tgcaactgga caaggtaaaa
     9421 ctgcaggacg tggacaaaaa ggtcaatggt ctcgttctgg tggtggagta agagtaggat
     9481 ttgaaggtgg acaaatgcca ctagctagaa gacttcctaa gagaggtttc aataacatat
     9541 ttaagaaagt ttacacagaa gttaatgttg aagttttaaa cagatttgaa aatggaacag
     9601 aaataactgc agaattatta aaatctacta aaactataag caaaataggt aaagacggaa
     9661 ttaaaatctt aggtgaaggt aatttagaga aagctttaac tgttaaagct gctaaattta
     9721 cagcttcagc tcaagaaaaa atagaaaaag ctggtggaaa agcagagtta gtataataac
     9781 ctgctaatct cctagccagt acaggcgggg tgaattagcg tgctgtcaaa attaaaacaa
     9841 gcttggaaga ttaaagcagt aagaaaaaaa gtaatgtata cacttatgat gatcgtgata
     9901 ttcagaatag gtactactat accagttcca ggcatagaca ctagtattat acaaaagatg
     9961 gtaggcggca atagtctact ttctctgtat aatatgttta ctggtggagc atttagtaac
    10021 ttttcactat ttgctttagg tataagtcca tatatcactg catcaatcat aatacaactt
    10081 ctaacagtag gttttgaaag tttagcagaa cttcaaaaat ctggtgaaga aggaaagaaa
    10141 aaaatcaata agtacactaa atatacagca ctagctctag cagttgtaca agctttggga
    10201 attacattag gaattgtaag aagtgcttta atatcaaata gtgtgttttt tatcacaact
    10261 gttgtaataa cattagtttc agcaagtatg ttagtaatgt ggattgggga caaaattact
    10321 gaaaaaggaa tcggtaatgg tagttcagta ataatatttg caggtataat atcaagaata
    10381 cctacagatg ttattaaaat atcgcaacaa gttaaatctg gcgaagttgc accttgggtt
    10441 atagtgattc tagctgtagt tatattactt acagtaacag gagttacatt tatacaagaa
    10501 gcaactagaa agatacctgt acaatatgct aaaagagttg ttggaagaaa aatgtatgga
    10561 ggacaaagtt ctcatatacc aatgaaagtt aaccagtcag gagttatgcc tataattttt
    10621 gctagttcac ttttagcttt ccctcaaaca atagctatgt ttatgggtcc aaatgctcag
    10681 gcttttgttc aaaaatactt aagtatggca acagaacaag gattttggac atatagatca
    10741 attgagattc tacttataat tttcttctca tatttttata caactgtatc atttaataca
    10801 gaagacatat ctaaaaatat gaaaaataat ggtggattta taccaggtat aagaccaggt
    10861 gagcctacta tggattattt aaatagaatt ttaactagat taactttagc tggagctact
    10921 ttcttagcaa taattgctat ggtgccagct cttacaacac attatatgaa agtaaatatg
    10981 agtctagctg ggacttcatt gctaatcgtt gttggagttg ctttggagct taagagacag
    11041 ttagagtcaa acttagttat gagaagctat caaggtttct taaaataaaa tggagatgga
    11101 taatatgaga ataatattac ttggacctcc aggtgctggt aaaggtactc aggcggcagg
    11161 aatagtagaa aaatacaata tacctcatat atcaacagga gatatattca gaaagaatat
    11221 aaaagaggga acagaacttg gaaaaaaagc taaagaatac atggaccaag gtttattagt
    11281 accagatgag ttaactgtag gtttagttac tgatagaata tctcaagaag attgtaaaaa
    11341 tggatttatg ttagatggat ttccaagaaa tgtagcacaa ggagaacatt tagatatctt
    11401 cttaaaaaat gctggtatat cactagataa agttgtcaat attgaagttg ataagagtat
    11461 attagtgtct agagcagttg gtagaagaat atgtaagtct tgtggagcta cttaccatgt
    11521 tgagtttaat cctcctaaag tagaaggtgt atgtgatgta tgccaaggag aattatatca
    11581 aagagctgat gataatgaag aaactgtatc taagagaata caagtttatc tagatgaaac
    11641 taagccttta gtagattatt atagcaaaca aggtataata gcagatataa aaggtgatca
    11701 agcaatagat aaagtatttg aagatattgt cgcagcctta ggaagtggaa aataaatgat
    11761 tatcttgaaa tcaaaaaaag aaatagaact tttaagagaa gcaggtaaaa tagttgctga
    11821 cactcatgaa gttttaagaa aagctatttc tccgggaata tctactttag aattagataa
    11881 aatagctgaa gagaatataa gaaaatacaa tgcagagcct tcttttaaag gatatggagg
    11941 ttttccagga agtatatgtg cttctatcaa tagagaggta gtacatggta ttccaggaga
    12001 aactatatta caagaaggtg acattgtaag tttggatata ggagcctatt ataaaggata
    12061 tcatggtgat tctgcgaaaa ctcacggagt aggcatgata tctgaagaag ataggaaatt
    12121 gatagaagta actagagaaa gcttctatga aggaataaaa tttgctaaat taggatatag
    12181 actttcagat atctcacacg cagtacaaac acacgttgag aaacatggtt tctcggtagt
    12241 tagagattta gtgggtcatg gagttggagc aaatcttcat gaagacccac aagtacctaa
    12301 ttatggactc ccaggaaaag gaccaagact tagagaagga atggttattg ctatagagcc
    12361 aatggttaat gcaggtcgtt atcatgtaaa gactctatca gatgggtgga cgactgttac
    12421 aatagatgga aaaaaatcag ctcattatga gcatacgata gcaattactg aacatgaacc
    12481 tttgatattg acaaagttgt aatttaaaaa gtaggtgaaa aaagtgctat cagataattt
    12541 aagtataggt caagttgtta aagtttcatt aggacgagat aaaggaaatt tattctttgt
    12601 agtcaaaata attaataatg aatatgtatt aatagctgat ggaaaaaaga gaaaacttga
    12661 caaacctaag ttaaagaaag tgaagcactt aaaaaaatat aattttatta atgatgaagt
    12721 tagaaaaaga gtagtatctg gacaagagat tacagattct tttttaagag ctgaacttac
    12781 taagttaaat tagataattt aacttattga aatggagggc tggttagtta atggccaaaa
    12841 aagatgttat agaattagaa ggtacagttt cagaagcttt acctaatgct atgttcaaag
    12901 ttaaactaga aaatggacat gagatattat gccacatttc tggaaagcta agaatgaact
    12961 tcataagaat tcttgaaggt gacaaggtga atgttgaact ttctccatat gaccttacaa
    13021 gaggaagaat tacttggcgt aagaagtaga ctatattaat atttagtcta aggagggatt
    13081 aataatgaag gttagaccat cagtaaaacc aatatgtgaa aaatgtaaag taataaaaag
    13141 aaaaggaaaa gtaatggtta tctgtgaaaa tccaaagcat aagcaaaagc aaggataaga
    13201 aattattact agcattttgt taaattttat agtataatac tatattgtga tgttaacaaa
    13261 agaacgttaa aatcgttgaa caatctggcg taggattggc atatgatgtg aagttttaaa
    13321 cactaaaaac tatgtgtagg aggtgcgatc atggcaagaa tagctggggt agatttacct
    13381 agagaaaaaa gagcggagat aggcttaact tatatatatg gtataggtaa agcaactgct
    13441 aacgaaatat tagctaaggc tgagataaat ccagatacta gaataaaaga tttatcagaa
    13501 gatcaagtta atgagttaag aaaagttata gatgacgatt tcttagttga aggtgactta
    13561 agaagagaaa ttgctttaaa cataaagaga ttaagagata taaaatgtta tagaggaata
    13621 agacacgcta aaggtcttcc attaagagga caaagaacta agactaatgc tagaactaga
    13681 aaaggtccta gaaaaacagt atctcgtaaa aagaaaaaat aaataaataa taactagatg
    13741 aaggagggaa aaggaaaatg gctaaaccaa aaaagaaagt tacacgtatt agaagaagag
    13801 aacgtaaaaa tatagaacgt ggtcatgctc atatacaatc aactttcaat aatacaataa
    13861 taactttaac tgacgttcac ggaaatgcta tatcttgggc aagttctgga caattaggat
    13921 tcaaaggatc aagaaaatca actccatttg catctcaaat ggctgctgaa acagctgcaa
    13981 aagctgcgat ggaacacgga ctaaaaagtg ttgaggtatt cgtaaagggg ccaggttcag
    14041 gaagagaagc tgcaataaga gctttacaag caactggact agaagtaact atgataaaag
    14101 acgttactcc aatcccacat aacggatgca gaccaccaaa gagaagaaga gtgtaattaa
    14161 taagagtaca ataggaggtg tagataaatg gcaagatata caggtgcatc atgtagacaa
    14221 tgccgtagag agggaatgaa attattcctt aaaggtgata gatgttatac agacaaatgt
    14281 gctatagtaa aaagaaacta tgctccaggt caacatggcc aaggaagaaa gaaagtttct
    14341 aactatggat tacaattaag agaaaaacaa aaagttaaaa gaatatatgg agttttagag
    14401 actcaattta gaaatttata tgaacgtgct gaaaatatgc ctggtaaagc aggtgagaac
    14461 ttattaagtt tattagagag aagattagat aatgtagttt acagaatggg actagcatct
    14521 tctagaaaag aagctagaca attagtgact catggtcatt tcactttaaa tggaaataaa
    14581 gtagacatac cttcattaat agttaaggtt ggagatgtta tagaagttaa agagaaatca
    14641 agatcttctg caaaattcaa aaatttagta gaagttaact caagaatagc tcctaagtgg
    14701 ttagaagcta atgtagaagg aatgacagca aaagtagttg gtgttccaac aagagaagat
    14761 atagatcttg agatagcaga gcacttaatc atagaacttt actctaaata ataagcacac
    14821 ttttaaaaat tattttaaaa ttttgtttac cctcagtgga ttactgaatt ttaaggaggg
    14881 ttttgtccat gatagaaata gaaaagccaa aagtagatat agttgagctt agcgaagact
    14941 atagatatgg taagtttgtt atagagcctc tagaaagagg atatggaata actataggta
    15001 acgcattaag aagaatatta ttatcatcat taccaggtgt agcagtgaat gctataaaaa
    15061 tagatggagt tcttcatgaa ttttcaacaa tacctggtgt taaagaagat gttactgaga
    15121 taatattaac tttaaaagag ctttcagcaa ctatagatgg tgaaggaagt agaacactta
    15181 aaatagaagc tcaaggacca tgctctatca caggtgcaga tataatctgt cctccagatg
    15241 ttgaaatatt aagtaaagat ttagcaatag ctacattaga tgataatgct aaacttaata
    15301 tggagatatt tgtagataaa ggtagaggtt atgtttctgc tgaagaaaat aaaacagaga
    15361 atgttccaat aggtgtttta cctgtggatt caatatatac tcctgttgag aaggttagtt
    15421 atcatgtgga aaacacaaga gttggtcaaa aaacagatta tgataaatta gtactagaag
    15481 tttggactaa tggtagtata aatcctcaag agggaatatc attagctgca aaggtattag
    15541 ttgagcattt aaacttattt atagacttaa ctgaacatgt aagtagtgtt gaaatcatgg
    15601 tagaaaaaga agaagatcaa aaagaaaaag ttcttgaaat gactatagaa gaattagatt
    15661 tatcagttag atcatataac tgtttaaaga gagcgggaat taatacagtt gaagaattag
    15721 ctaataaatc tgaggatgat atgatgaagg ttagaaacct aggtaagaag tcattagaag
    15781 aggtaataca aaaactagaa gaacttggat tgggattaaa accaagtgaa gaatagcgag
    15841 caaaggaggg ataagaatgg ctaagtaccg taaattagga cgtgaaacag ctcacagaaa
    15901 ccttatgtta agaaacttag taacttgctt actaagaagt ggaagaatag aaactacagt
    15961 gactagagcg aaagaaactc gtagaatggc tgaaaagatg ataactcttg ctaagagagg
    16021 agatcttcat gctagaagac aagttttagc ttatgttatg gatgaaacag tagttaataa
    16081 cttattcaca gatttagctc caaaatatgc tgagagaaat ggtggataca ctagaataat
    16141 aaaaataggg ccaagaaaag gcgatgctgc tgaaatggct tttatagaat tagtatagta
    16201 aggaaaggat taggcttgat ggcttaatct tttttttatt tttttgacta aggtatgata
    16261 taataatgtg taaaatgatt tttataagtt attgcttact aaaaggagta tgagttaatg
    16321 gataatatag taaaggtaaa taatatttcg tttgagtata ttacagatga agcaaaactt
    16381 aaagcaatag ataatttaag cctagatgtt aaaaaaggag aatttgttgc gataatagga
    16441 cataatggtt ctggtaagtc tactttatct aaaaacttaa atgctattct tatgcctact
    16501 gaaggaaata ttcttattga tgatatggat actaaagaag aagaaagatt atgggatata
    16561 agacaaactg caggcatggt atttcaaaat ccagataatc aaatagttgc tactatagta
    16621 gaagaagacg ttgcatttgg acctgagaac ttaggtatag aaccaaaaga aataagacga
    16681 attgtagagg agtctttaaa aagtgtaggt atgtatgatt taagagatag acagcctcat
    16741 ttgttatcag gaggtcaaaa gcaaagagtt gcaatagcag gaattattgc tatgaggcct
    16801 aaatgtataa tatttgatga agcaacagca atgttagatc catctggaag aaaagaagtt
    16861 atgaaaacaa taaaaagact taataaggaa gaaaatataa ctgttataca tataacgcac
    16921 tttatggaag aagcagtaga agctgataga gttgtagtta tggaaaaagg aaagaaaatc
    16981 ttagagggaa ctcctagaga agtattcagt aaaataaaga tgttaaagga aatcggttta
    17041 gatgtacctt gtatgacaga gttatctagt ttattaatag aagaaggtat aaatattagt
    17101 agtgatatat taactgtgga tgagatggtg atggaattat gtcaattata gtaaagaatt
    17161 taacacatat atataatgaa gggatgcctt ttgcaagtaa ggcacttgat gatatttctt
    17221 ttgagattaa agatagagat tttgttggtc ttataggtca tacaggctca ggaaaatcta
    17281 cattaataca acatttaaat ggattactaa agccttcctc aggagaaata tttataaatg
    17341 attttaatat aacagataaa aacttgaatt taactgagat tagaaaaaga gtaggggttg
    17401 tatttcaata tccagaatac caattgtttg aagaaacgat tgataaagat atagcttttg
    17461 gaccctctaa tttaggctta gaggagtctg agatacataa cagggtaaaa gcatcaatgg
    17521 aagctgtagg acttgattat gagggattta aagacaaatc tccttttgaa ttatctgggg
    17581 gacaaaagcg tagagtagca attgctggag ttatagccat gaacccagaa gttctcatat
    17641 tggatgaacc aactgctggt cttgaccctg gtggaagaga tgaaattttt aacttaataa
    17701 aagacttaca tgaaaaaaag aatatgacta taatactatc atcacatagt atggatgata
    17761 tggcaaagtt agcaaaaact ttaattgtta tgaatcatgg tagcgtagaa tttatgggaa
    17821 ctcctagaga agtgtttaaa tcaaatgcta gtaaattaaa agatatagga ttagatattc
    17881 cacaagtttt agagcttgca ttgaaattga gagaaaaagg ctttgacatc agtgaggata
    17941 tattgacttt agaagaagca aaacaggaaa tattaaaagt tgtgagagga cgaggattat
    18001 gttaaaagat ataactatag ggcaatacta cccaacaagt tctgctattc ataaattaga
    18061 tccaaggata aagcttgtag cgacaattgt atttatggta tctatatttg tagtaaataa
    18121 attttggcct tatatagtag ttttattatg tttattagct atgataaaat tggctaatat
    18181 accagtcaaa tatattgtta agggtgttaa acctcttaaa tggattattt tgtttacatt
    18241 tcttataaat atttttttct taccaggtga tgaaatatgg tcatttggat ttttagctat
    18301 tacaaaacag ggtctaagac aagcaatttt tatggcaata agattaatat ttttagtagt
    18361 tggtacatca ttgcttactt taacaacttc accaatagaa ttaactgatg gaatagagag
    18421 attgttgaat ccatttaaga aaattggttt gccagttcat gaacttgcaa tgatgatgac
    18481 aattgcttta cgttttattc ctacattgtt agatgagaca gacaaaataa tgaaggctca
    18541 gatgtctaga ggtgcagatt ttgaaagtaa gaatcttata aatagagcaa agaatttagt
    18601 accacttttg gtacctttat ttgtaagtgc atttagaaga gctgatgaat tggctatggc
    18661 catggaggcc agatgttaca gaggtggaca taataggact aagatgagag agtctgttat
    18721 atcaagaagg gattatatgg catgtgtatt tcaggttgtt tatctgggag caattatagc
    18781 tactagattt atagcaattt aattaataag ttatagatat aatgaggtaa agtatgagaa
    18841 atataaaaat taaaatccaa tacaatggta agaattattg tggatggcaa aagcaacctg
    18901 attctttagg gatacaaggt acaattgaaa gggctatata tgatataaca aaagaagaaa
    18961 ccagtcttat agggtctggt agaactgatt caggagttca tgctattggt cagattgcaa
    19021 attttaaaat taattcaggt ataagtatag aaagtatacc aatggcacta aatgccaagt
    19081 tgccaaaaga catatcagtt atagaagcat gtgaagtaaa tgatgatttt cactccagat
    19141 acagtgcaaa aggtaaaaca tataaatatt tagtatataa tagcaagttt agaaatccaa
    19201 tacttagtga gatatcatat caagtaaaat atgaacttga ttttgataaa atgtgttcag
    19261 aagcaaaaag tttattagga acacatgatt ttaaaggttt tatgagttct ggttcatctg
    19321 taaaagatac agttagaacg atatatgata ttgatataag taaaaaagat gacttgatta
    19381 cttttgagat tagtggtaat ggttttttat ataacatggt gaggattatt gttggaacac
    19441 ttgttgatat gggtcgtggc agaattaacg aaccattttt agatataata caatcaaaaa
    19501 ctaggtcaag atgtggacat acagctccag cacaaggatt atttctcaaa aaagttcatt
    19561 attaacttga cacaccaggc tacatgtatt aaaatatatg agaatgtgtt aataagtcca
    19621 cttgccccgg acttgacata aacggtaaaa aaattttatg aaaagtagtt aaggagggac
    19681 agttatgaaa agttatatag ctaagccagc tgatgtacaa agaaaatggt acttagttga
    19741 tgctgaagga aaaacattag gtcgtttagc aacagaaatt gcgacagtat tgagaggtaa
    19801 acataaacca acatttactc ctcacgttga tggtggagat tttgttgttg tagtaaatgc
    19861 agaaaaaata gttttaagtg gtaagaaatt agatcaaaaa tattacagat accatactgg
    19921 ttatgtaggt ggattaaaag aaatatctta cagagatatg atggataaga aaccagaaga
    19981 agttatatca catgctgtaa gcggtatgtt acctaaaaat aaattaagaa gcagaatgat
    20041 gactagatta agagtatttg caggtgctga gcacactcat gcagctcaaa atccagaagt
    20101 tttaaacttt aaataagatt aataggtgaa aggaggagtt tataatggct aacgttcaat
    20161 attatggaac tggaagaaga aaaagttctg ttgctagagt aagactagtt gcaggtgaag
    20221 gaaatatatt agtaaatgga agagcattag aaaattattt taattatgag acattaataa
    20281 gagatgttaa acaaccatta gttttaactg gtaatgaaaa taaatatgat gttatagtaa
    20341 aagttgaagg tggaggattc actggacaag ctggagctat aagacatgga atatctagag
    20401 ctttattaaa agctgattta gatttaagac ctgctttaaa gaaagaaggt ttcttaacta
    20461 gagatgcaag aatgaaggaa agaaagaaat acggattaaa agcagcaaga agagctccac
    20521 aattctcaaa aagataatta cttatttttt tggaaaagca aaatccttta tttactgtta
    20581 ttcagaaagt attggtattt taaaaatgtt gggttaagtt aattattgat atataataaa
    20641 ataataagat atcttattag gatgtttttc attaagaaga acatc
//
LOCUS       DAEPLF010000047        20683 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEPLF010000047 DAEPLF010000000
VERSION     DAEPLF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20683
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.047"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            48..1925
                     /locus_tag="KR314_001546"
     CDS             48..1925
                     /locus_tag="KR314_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0327936.1"
                     /translation="MVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTG
                     AYEGTINYNQDPEYLNVWELQGITINSKNNPKTLNGQDLEKMGLNLKDYNVTQECIIE
                     DITSRKDVNKYLRKTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGI
                     ISTPLATTYNAPILLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTV
                     NASQTRLNGSNRYETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPI
                     ILTDKDSITDNTYKWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGAD
                     RHETNANVIKKFYTEDEIEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYH
                     KDNLEAKSANKVYKIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGS
                     APGNSSGGMIEKDYNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPD
                     LFTSIHYNGSTNKQGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRV
                     LPSDSTKDYLYVLRSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSL
                     K"
     gene            2153..4066
                     /locus_tag="KR314_001547"
     CDS             2153..4066
                     /locus_tag="KR314_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327937.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTDNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEINTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEENVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLRMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            4059..6278
                     /locus_tag="KR314_001548"
     CDS             4059..6278
                     /locus_tag="KR314_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBH0327938.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEIALEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLISENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            6387..7217
                     /locus_tag="KR314_001549"
     CDS             6387..7217
                     /locus_tag="KR314_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBH0327939.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            7207..10674
                     /gene="addB"
                     /locus_tag="KR314_001550"
     CDS             7207..10674
                     /gene="addB"
                     /locus_tag="KR314_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBH0327940.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIEN
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDDYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSTICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            10674..14501
                     /gene="addA"
                     /locus_tag="KR314_001551"
     CDS             10674..14501
                     /gene="addA"
                     /locus_tag="KR314_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBH0327941.1"
                     /translation="MSSPKWTKEQLEVIGSRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIANISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDIDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNETESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDSPDSDY
                     YSEIEKKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            14547..15758
                     /locus_tag="KR314_001552"
     CDS             14547..15758
                     /locus_tag="KR314_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBH0327942.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVIATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            15745..19272
                     /locus_tag="KR314_001553"
     CDS             15745..19272
                     /locus_tag="KR314_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0327943.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNIQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFVVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKDLKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRS
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVINITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            19403..20656
                     /locus_tag="KR314_001554"
     CDS             19403..20656
                     /locus_tag="KR314_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBH0327944.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
BASE COUNT         8988 a         1948 c         3669 g         6078 t
ORIGIN      
        1 ctatggaaaa taacgctgat ttaaatataa acttagagaa aaaaagtatc gttttaggta
       61 gcaaatcaaa agttagtgtc aaatttaaag aaaaaccaga tgcagatagc attacattaa
      121 agtataaatg ctatgacatg ccattggata caactctaaa ttacaatcaa tcaactgggg
      181 catatgaagg aactatcaat tataaccaag acccagaata tctaaatgtt tgggaactac
      241 aagggataac aataaacagc aaaaataatc ctaaaacttt aaacggacaa gacctagaaa
      301 agatgggatt aaatttaaaa gactataatg taacacagga atgtataatt gaagacataa
      361 cttctagaaa agatgtaaat aaatatttga gaaaaacttc tgcacctatt acagaactta
      421 caggaagtga tagatatgaa acagcagtta aaataagtaa agagggctgg aaaaatggtt
      481 cagataaagt agttataata aatggagatg taagtataga tggcattata tcaactccgc
      541 tggcaaccac atataatgct ccaatacttt tggttgaaaa aaacaatgtg cctaatagtg
      601 taaaatcaga attaaagcgc ctaaacccta aagatataat tataattgga gatgagaatg
      661 ctatttctaa aactactgct aatcaaatta aatcgactgt aaatgctagt caaacacgtt
      721 taaatggttc taatagatat gagacatctt tattgatagc aaaggaaata gataaaaatc
      781 atgatgtgga aaaagtatac ataacaaatg ctaatggcgg agaagtggat gcacttacta
      841 tagcagcaaa agcaggtcaa gacaagcaac caattatatt aactgataaa gatagtatta
      901 cagacaatac atataaatgg ttaaagagtg aggatttaca aaatgcttat tttataggtg
      961 gtcctcaaat gatatcaaca aatgttataa ataaggtaaa tggaataact aaggatagtg
     1021 ttactaataa tagagtatac ggagcagata gacacgaaac aaatgcaaat gtaataaaaa
     1081 aattctatac agaggatgag atagaggctg ttttagtagc taaatcagat gtacttgttg
     1141 atgctttagc agcaggtcca ttggctgcga acttaaaatc tccaatactt ataacaccaa
     1201 agacgtatgt atctgcatac cataaagata atttagaagc taaatcagct aataaagtat
     1261 acaaaatagg tggaggattg acttctaagg taatgagctc tatagcatca tcattatcta
     1321 aacacaatac gactccaaca gaaccaggaa atagtggggg caagacagtt atgattgacc
     1381 cagggcatgg tggttcagca cctggaaatt catctggagg aatgattgaa aaagattaca
     1441 atttaaatac ttcacttgca acaactgaat atttacgttc aaagggattc aatgtaataa
     1501 tgacaagaga cacagataag actttatctc ttggaaatag aactgctcta tctaattcat
     1561 tgaaaccaga tttatttaca agtatacatt ataatggctc aactaataaa caaggtcatg
     1621 gtgtagaagt attttataag cttaaagata aaaatggggg gactactaaa actgtagcta
     1681 ccaatatatt aaatagaatt ttagagaaat ttaaacttac aaatagaggt ataaaaacaa
     1741 gagtacttcc tagtgattct acaaaagatt atttatacgt tttaagaagt aatgatatgc
     1801 cagctgtact tgtagaatgt gcatttttgg ataatgaaaa tgatatgagt ttaataaact
     1861 catctgcaaa agtaaaagaa atgggtacac aaataggtaa aggaatagaa gattcattaa
     1921 aataaatgga aataaaaata tttaaaaact taatgtaaat ttatactaaa gtcaaaaaat
     1981 gaaatataag tgtatcaata gtaagactgt atgtacattt gcacatgtga atgcaagtat
     2041 atatacagtc tttttttgta gttaaatgca ataatttgat tgtgttttgt aaaatacaca
     2101 atatgatata attagataat atacacttaa tttaattgag gaaggtgatt atatggaata
     2161 tttatttgaa aaaagtagtt taaaagaata tggaaatgaa gctttagata aatatagaga
     2221 attattagat aaaactgata ataattcaag gtgtattctt ttattagtac caaacaataa
     2281 cacaaagata cgatatgaaa gggctctaag attaaattat agtgaagaac tgaaaataac
     2341 aacatatata agttttgtga agaaagaatt agttaaatac tggccactaa taatagaaaa
     2401 atgtgatgaa ataaatacaa aggttgtatc tccaacattt atatcaaata gtttaagtga
     2461 atatttaata gtgcaaaaag ttaaggaaaa aaggatacaa gaaggatatt ttgaagatat
     2521 tacaggaacc aataaatcca tagcaaatag tataatgact aacataaata aatctgcatt
     2581 taatttaatt gattttgatt ttattggaga aaaaatatat tcatcaaaga aaaataaaga
     2641 tagtatatat agattttcat acactcagat ggatgaaatt atatcatatt atataaattc
     2701 gttactatca aattcaattt tagataatgc attaagcata tatttataca atcaatacct
     2761 acttaatgat gatttttatt taaaaaactt atttacagaa gtgagatata taattgtgga
     2821 tagtcttgaa agctcaagta atgcagaagt tgattttata acagaagcat tggataatac
     2881 attacaaagt tatatatatt ttgattatag tagggattat tctgtattta ataatgtgga
     2941 tatagactat ataaatgaga aaataatatc aaaaattaag cctaaagaag aaaatattaa
     3001 aaagaaggag attagaatag aagatttata cttattacct gcaaatatag aattaaatca
     3061 atcaagtcaa ttatacaatg aaatgttaga tttaataagt gaaaaagtta tatctcttat
     3121 agaagaaaat gtatctccaa gagatatagc aattatatct cctataaaca atagtatatt
     3181 ggagcatcaa attagagatt ctttgataga aaaaaatata gatgttttta gtactaagaa
     3241 ggataaaaaa gctgtagatt atccatatgg gaatgcattg gtagtggcta catgtatatt
     3301 ttatggatat ttagatttta taaaagatga tgaatactta agctttttag aaactctttt
     3361 agaaataaat aggataaaag catttaagat ttttaatgaa acaagacatt taagtattga
     3421 taatatagat gaacatagtc agtatcgtga tattttacag tatattgaag aaaaaaagaa
     3481 gagtgatata aaaatacatg aatttttaac tcaattttac atagataaaa tgttaaattt
     3541 aaaagaaggt aaaaagaatg ttgggttatg taaaaagata atagctgaaa gtgagtcttt
     3601 tagtgaaagt ttaagtctac ttaggatgaa aaaagaaaag atatttatag aagctttaaa
     3661 aagtacaata aacgattatt actctgttgt cgatatagaa gagttgaaat ccaaagacaa
     3721 aatagtaata actactcctt attcatatat atcatcaaat atagatagaa gtatacaaat
     3781 atgggtagat ataggaagta atgcttggaa tatgaaaata gaaaaagata taagtaattt
     3841 aattgtactt agaaaatctt ttgaagaaaa aaagatttat acaaatgaaa tggaagaata
     3901 ttataaaaaa tattatctat acaatacagt atataatttg ttattaaatg caaagaaaat
     3961 ctatgcattt aaaagtgaat atgcgattaa tggttatatc caagagagta tattatatag
     4021 tatactttta aaaatctcac ataaaggaga taaaaattat gattaatata aattatagag
     4081 aagaccagat accaataata aattacgata atggtactat ggcagtacca gcagtaccag
     4141 gtgctggaaa aacatttata ataacaaatt tagtagctaa actattactt gaacagaaac
     4201 ataagggtgg taaaatatta attcttacat atatgaatag tgctgtaaat aattttaaag
     4261 gtagaataag aaagattctt gaagagaata aaatagatga tactaattcg tatgaagtaa
     4321 tgactataca cagtttggct gtaaaaataa taaaggaaaa gccagaagtt gtaatgttaa
     4381 gtgaggattt taacatagct gacgatttgc aaaaaagtat tatattaaat gaatgtataa
     4441 gtagatttag atttgaaggt ggagaaagag cttttagatg gtttttaaag gagcaaaaag
     4501 atgaacgctg gaaagaaata gctcttgaag cctgggaaag aggttttttt gaatttgcag
     4561 gaaatgcaat aagtgaactt aagtataagg gaatttctcc agataaactt gaagaaatat
     4621 tgtcaagagg acacaaagga atgcttaaaa ttattttacc aatatataaa ttgtatgata
     4681 gaaaattaaa gcaaaatgga ttgctagatt atgatgatat attaatttta gcagaaaaaa
     4741 ctttaagctt agacgaaggg cttagaaaga agtttcaaac tagatacaaa tatatatttg
     4801 aagatgagtg tcaggactca aatgaaattc aaggtaatat aataaaaatt atatctagtg
     4861 aaaataaaaa cctagttaga gttggagata taaatcagag tataacagga acattttcat
     4921 catcagaccc taaatttttt aaagaattta taaatagtgc agatttctgt tataggatgg
     4981 atatgtcaaa tcgaagttca aaagatatac ttgatttagc aaacactctt gtcaagtatg
     5041 ttacaaagga atttagacaa gaagaatgta gacatgctct tgaagatatg caaataaaaa
     5101 ctgtgccaaa tggaatggga tataaagaaa atcctaatcc agaacattat aatataaatg
     5161 taaagtggta tgagagttgg aaaattgaaa tagagcaaac tgctagatac gttaagggta
     5221 taaaaaataa atatccagat aaaagtatag gtatattggt tccatttaat gaacaagtga
     5281 cacaagttgc taaagaactt agagagaata atctaacctt tgaagaatta ggaccaaatt
     5341 ctttgaataa aaggaaggtt ataaacaaca tagcatatat tattgatttt atgttacatt
     5401 gtgatgatgt agaaaagtta atagttgtac ttaataaagt ttttatagat actgataatg
     5461 aagaagaaat aaagtatttt ttagaagaat taaaaaacta ttctaccgaa gaaattattt
     5521 ataattttga atttagtaag ttagataatg atttaataga atttaaagaa gaaagtgaca
     5581 tatatttaag ttttaaaaga ggaatagata ttataaaaca gatattagaa tatccaataa
     5641 taaggttaga cctcttagtt ttgtttattg ggaaaaaact taatttagaa aaagaagata
     5701 aagctgtact ggattatata tctttttata taaagtatct aatatcagaa aatataaata
     5761 tggatttaga agatgtatat aatttattgt ttgatgtaaa gaatagggtg tttaatcata
     5821 taatagatgt ggtatatgag ataaatggat atgaaccaga gcccggtagt ataacactat
     5881 gtaactatca taagtcaaag gggatggaat gggattgtgt gtttttacta ggtcttgttg
     5941 aatataattt ccctgacaat attaatcaaa agtttcagag tgataaatgg tatctaaaag
     6001 aaaaatataa aaaccctatg gcaattatta aatcagaagt tgaagctata ttaaaaggaa
     6061 gtatttcaac agattatgct cataaaacga agattgattc tataaatgaa aagattagac
     6121 ttttatatgt agggattact agagcaaagg aaatgttagt actttcagga agtgcttata
     6181 gagatgaaag tgatataggg aataagagaa aagagcaaaa accatgtata tatttaagca
     6241 gacttaatca acatattatt gaaaaaaggt caaattaata tataataagt tattaaaaga
     6301 ttgcttgaaa tatatctaaa aggggataaa acaatatgta cagtaagtaa ctactaaaaa
     6361 ataaagttaa gagagaagtg atatgagtga acgataaatt aaaatatttt tcttatagtc
     6421 aaaattcaat caacacatat aaatcatgtc cattgaaatt taaatataaa tatattgata
     6481 gaataaattg gaaaaatgat gatgttggta gtagagagta ttatgaaact ttaaaaacag
     6541 gaagagattt tcatttgatt tgtgaaagat attttagcaa tatacctctt ggaatctatt
     6601 ttaatgagga tgataaaaat agtaaaaagt ttttaaagtg gatggaaaat ataaaaaaag
     6661 ttgttcctat tgaaaaagga aaaacgtatt tgccagaata tgaagtaagg atgaccttaa
     6721 atggggatat aatacaggca aagtatgatt tagttgtaat tggaaataac aatatagaaa
     6781 tatgggattg gaaaaccgag tctaaaaaga ttgactataa acacgtagaa aatagaatac
     6841 aaactattgt ttatatgttt ttagcaaaag aagtaatacc taaagtttta aaaatggaca
     6901 taaatgttaa agatataaat atgagatatt atcaacctga atttgatgac ttaccaatta
     6961 ccattttata tgatgaagaa aaacatgaag caaatagaaa taaaattcaa aattatataa
     7021 gtatgataaa aaacactaat tatgaagaac atacatacga agacaatcta tacaatgata
     7081 tagaaaaggt atataataaa gaagaacgta tgtgctatag aaataaaaag cattgcaagt
     7141 attgtgaatt taataagtta tgtaatggca aagaaataga ttatagtatt ttggaggcgg
     7201 aaatttatgg gacttaggtt tgtattaggt agaagtggaa gtggaaaatc cacctatata
     7261 ctagatgaga ttaaaaaaga agctcaaaaa aatgaaacga catcaattat acttttagtt
     7321 ccagaacagt acacatttga ggctgaaaat agagtaagta agctattctt aggaaaagaa
     7381 aaagataagt atctaagagt tagggtactt agctttaaga cactaagtaa tatagttttt
     7441 tctcaagtag gaggattgac agatgtaaat ataaattcta gtggaaaagc aatgatggta
     7501 tatagagcta ttgaagatgt aagtgaagaa ttaaatgtgt tttcaaaatc aaaatcgcag
     7561 tctggttttg taagctcaat tacagatatg atatcagaaa tgaaacaata caatataagc
     7621 cctgaaatgt tagaaaatat atctggagaa ttggataatg aaactttaag tttaaaatta
     7681 aaagatataa gtaaaatata taattctttt gaagggaaat tgcatgaaaa ttatgtagat
     7741 gctcaagata tgttaacttc attggcaagt aaaattgaat taagcagtta tttagatgga
     7801 gcttgtgttt atatagatga atttacaggt tttacaccaa atcagtacaa tgtcataaag
     7861 agtatattaa ataagtctaa aagtgttaat atctctttga ctgtagatga tatcaactat
     7921 attgggtaca gtaaatctga tatgttttct agaacgaaat ttacttattc aaaattgact
     7981 caattgtgca acgaagaagg cataaaaata ttgccacaag taaatttaaa cacaggtgtt
     8041 ataaaaagat ttgaaaaagt taaggaatta cagcacctag aaagatttta taatgcttat
     8101 ccatataaaa tatattcaaa tccaactgaa aatataaaga taaaagagtt taacaattta
     8161 tattcagagg tggaagagat agccagagaa attgtacatt tagttagaga taagaatgta
     8221 agatatagag atataaccat tgctacaaga gatttaaata gatatgattt tttagtacac
     8281 tctatattta atgaatataa tataccaaac tttatagata agaagagaga ggcaaaaagt
     8341 aatccaatag ttattttaat tatttcagct ttagaaatga agaatagacg atatgggtat
     8401 gaaactatgt ttaggtattt aaaaagtgga cttataggaa ttgataatga tgatataaat
     8461 ttactagaaa attatgtgtt ggctaatggg attaaaggta aaaaatggtt tgatgaaaaa
     8521 tgggattata gaataacaca aagtctttca ggtcaagaaa gtgaatttga actggaatta
     8581 aaagaaaaaa taaatgaaat aaaaaataga gtattagagc ctatagtaat tttacaagaa
     8641 aaattaagag gaaaaaatag agtaaaagaa atctgtagat atatatatga atttttatta
     8701 gatataaata tgccagaaac tatagaaaat ttaatagtaa attttaagga taaaggggaa
     8761 cttgatgttg caaatcaata ctcacaagtt tgggacatag tagtggacat attagaccaa
     8821 atggtggaac ttatgggtga tgaaataatt tctttagaaa aatttataaa attaataacc
     8881 ttaggctttg atgaatatga attaggactg gttcctccta gtatagacca ggtacttgta
     8941 agtagtgtgg atagaatgaa aaatccagac acgaaatatt tatacctagt tggaactact
     9001 gatggtgtgt ttccactgat tacaaaggat agtggaatat tgagtgataa tgatagggaa
     9061 agtcttggaa acaaaggaat cgaagttgat attgatagta agactaggag ttttgaagaa
     9121 cagtttcttg tatataaagc tcttacttcg acatctaaga atttaaccat aacatatcca
     9181 atatcagacc atgaaggtaa aacgcttaga ccatcaataa ttatatcaag acttaagaag
     9241 atatttccaa atatagaaaa taaaagttat ttagtggaag aaaataaaag cactgataaa
     9301 gatatactta aaaaaataac ggttaaatcc cctacattta atgaacttat aaacgtaata
     9361 aaaaattatg acagtgatga ttacaataca gaagagatta atagtatttg gttagatata
     9421 tataggtact atttaaaaga tgaaatctat agttctataa caaaaaaagt tataaaaggt
     9481 cttagttata ctaatcaagt tcataagatt gaagagaaaa aaataaggtc attgtataaa
     9541 agtaattctc ttagcatatc taggcttgaa aaatatgctg aatgtccatt tgcttatttt
     9601 atacaatatg gtctaaaagc aaaaaagcgt aaagagtatt catttacacc accagattta
     9661 ggaactttta ttcataatat tttagatagg ttttcaaaag aacttttaca agataattta
     9721 acttggagag atattgatga aaaatatata gaattaaaga taggtataat agttgatgaa
     9781 ataattttaa agataccagg atatatatta aatagttctg agaggtataa ataccttgca
     9841 tatagattaa agaatatgtt gacaactgct ataactataa ttagtcagca aataaaacaa
     9901 ggttcatttg aaccaataga ttatgaagtt aaatttggcg acaatggaaa atatccacct
     9961 ataaaaatgg tgttagaaaa tggacaagaa gtaagtttaa ttggacaaat agatagagtt
    10021 gatgaatttg aagaaggaga aaataaatat ataagaataa tagattataa gtcaggcaat
    10081 aagtccataa gtctaacaga aatatattat ggtctacaat tacaattact tgtgtattta
    10141 gatgcaatcc ttgaaagtgc aaaggatgaa gatataaata taaatccagc tgcaatacta
    10201 tattgtagaa taaataatcc aatagcaaaa tttaatgaag ataaagatga tgaagaaata
    10261 caagaagcaa tattaaaaga acttagaatg aaggggcttg tagtaaaaga ctctcacata
    10321 gttaaagaga tggataaatc attaatagat ggagaaagga aaaactcttt agtaatacca
    10381 gtaggtctta ctaaggatgg aaatgtaggt aaaagtactt cagcaataag ctatgaggat
    10441 ttcaaactgc ttagaaaata tgtaaggcat gctataaagg atttatgtga agaaatgtta
    10501 agtggagaaa tacgtatagc tccatataaa cataaagatg gaacatcatg tgacttttgt
    10561 gattactcta ctatatgtca gtttgattca actataaaag ataacaagta taaaaattta
    10621 aataataaga gtaatgaaga gataataaaa atgatgaaag gagatgttaa ttagtgagtt
    10681 ctcctaaatg gacaaaagag caattagaag ttataggaag tagagaatgc aatttattgg
    10741 ttgctgctgc tgctggttca ggaaaaacag ccgttctagt tgagcgtata attcaaatga
    10801 taacaagtag agaaaatcca attgatatag ataagttatt agttgttaca tttactaatg
    10861 ctgctgcgtc agaaatgaga gagaggatag gggatgctat aggaaaagcc ttagatgaaa
    10921 acccagaaaa taaacattta caaaaccaac ttgttttatt aaataaatct agtattacaa
    10981 caatacactc attctgtttg gatgttataa aatcaaactt tcatagaata aacttagacc
    11041 ctaatttcag aataggagac caaactgaat gtgctatttt gaaacaagaa gcaatagagg
    11101 aagtatttga agatttatat gaagaaagag atgaggggtt tttaaattta gttgaaagtt
    11161 atgcagaaag aggaggagat aaagaagtac aagatattat cttgggaata tattcatttg
    11221 caatggcttc tcctgaacct aaaaaatggt tgattgactc agcagaacga tttaatattg
    11281 atgaaaattt tgatttttca cagtctattt gggctagagc tatattagac actgtaaaaa
    11341 tagaaattaa tggtctttgt ctaaatatgg aaagggcatt aaaagaagtg gaatcaatag
    11401 aagaattaga aacttttgca gaaaagttat ccgttgaata taaaaaaatt gctaacattt
    11461 cacaagcatg taataaatct tgggatgagg catataaaaa gatggcaagt atgtcctttg
    11521 aaaattatgt aaaaggagta aaaagaattt caaaagatgc tccttcgtat ataaaagaat
    11581 caaaagaaaa ggcaaaaact ataagagata agactaagaa gtctttagaa agtatagtaa
    11641 gtgctacatt caataaagat aatgattcta ttagagaaga aataaaatat ctttataaca
    11701 tagttaaacc aatatctagt gttgtactga gatttgaaga ggaatatagc aataagaaaa
    11761 gagaaaaagg aataatagat tttaacgata tagagcattt tgcattaaat atacttacag
    11821 atatagatga aaaaggtaat attgtacctt ctgatatagc cgttggatat agaaacaagt
    11881 tttatgaaat atttatagat gaatatcagg atagtaatct agtgcaggaa gtactgctta
    11941 aagctgttgc aaatacagaa actccaaata gatttatggt tggtgatgta aaacagagta
    12001 tatatagatt tagacaagca aagccagaat tatttttgca aaaatataat aactataatg
    12061 ataaaaaggg aagttcccat agaaaaataa tgctttacaa aaattttaga agtagagaag
    12121 aagttgtaga tgctgtaaat tatatatttg aaaatataat gaatgagaat ataggagaaa
    12181 ttgaatatac agaaaaagaa agactaaatt taggtgcgaa ttttaatgtg gatacagacg
    12241 agaaatctat tattggtgga gcaacggaga tacacctaat acaaaaagat aataaacttg
    12301 atgatgatat tataaatgat aaagacgata gaattaataa caaagaaaat gaaatagagg
    12361 aagaagaaaa attagataat atacaacttg aggctagaat ggttggaaac attatcaaag
    12421 atttaatgaa agtcaatgaa gatggaaaaa ttcaaaaggt ctatgataag ggaatagatg
    12481 gatatagacc tgtggaattt agagatatag ttattctttt aagagctaca tctgcatggg
    12541 ctccagtgtt tgcagatgaa ttgatgaata tggatatacc aacatatgct gatgttggag
    12601 tggggtattt tgatacgatt gaaataaaaa caatattgtc attacttcaa ataatagata
    12661 atccaatgca agatatacct cttatatctg tattaaaatc acctatattt ggatttacac
    12721 cagaggattt gattgatatt agagttcaga gtaaagataa aattttttat gaggttttaa
    12781 aaagtacagc agagtatgat ggatttacag attctcaaaa tgaaactgag tctgagttta
    12841 taccaagtga agagtgcata aacaaaagta aagatttttt aattaagcta aaggaattta
    12901 aagaaaagtc aatgtacatg agtacagatg aatttatatg gtacttgtac acaaggactg
    12961 gatactatgc ttatgttgga gcattgccag gtggaagtca aagacaagct aatttaaaag
    13021 tattgtttga gagagcaaaa caatttgaag aaaccagtct taaaggaata ttcaactttg
    13081 taaattttat agagaaatta aaaaaatcta gttctgatat gggaagtgca aaaacacttg
    13141 gtgagaatgc aaatgttgtt agaataatga gtattcataa aagtaaaggt ctagaatttc
    13201 cagtagtcat atgctctgca atgggtaaaa atttcaatac tcaagatttt aaaaagagta
    13261 ttttatatca tcataattta gggtatggac ctcaatttgt agactatgag agaaggattt
    13321 cttttccaag tatagccaaa gaagcattaa aaagcaaaat aaatatagaa aatttatcag
    13381 aagagatgag agttttatat gttgcattta caagagctaa agaaaaattg attataactg
    13441 gttctactag aaatatacaa gatagtataa agagatggtc aaatggaatt gaaagtttag
    13501 atacaatatc acaatacgag atattaaaag gtaaaaattt tttagattgg ataatgcctt
    13561 gtgtattaag acatagggat ttatctaatc tactagaaga agtaggatta gacgcagttt
    13621 ttaatgttga acataattca aaatggtatg gaaagttgtg gaataagaat gatattttag
    13681 tggaaaagaa gagtgatgaa gaaaaagaaa gtattgaaga aattcttgaa aaaatagatg
    13741 tagatagtcc ggacagtgat tattacagtg aaatagaaaa aaaattaaac tatatatatc
    13801 catatgaatt tagtacaaga aaacctgcta ctatatctgt tacagaaata aaaaagatac
    13861 aaaataatta tgaagaagag ttaataaata ctatatttga acaaaaagta atattaaaaa
    13921 agcctctatt tattcaaaat gaagaagaaa gagaaaaaat aagtggaaca gaaagaggta
    13981 caatagtcca tttggttatg gaggttttag acttaaaaaa tgtaagtagt gtaaatgata
    14041 taaaatcaca aataagaggt tttgtttcaa aaggtataat aacagaaaaa caagctagta
    14101 tagtaaatcc atataaaata tataaatttt ttgcctctaa cataggaaaa agaatgttga
    14161 atgctgaaat tataaataga gaaaaatcaa tttatgcaca agtaaatatg aaagacatat
    14221 atatttatga aaagcttata aacaatgatg ataaaaaact ttatgataat gaaagtgtca
    14281 tgttaagagg tatagttgat gcttattttg aagaagataa tcaaatagtt ttggtggatt
    14341 ataaaacaga ttttgtaaat gaggaaaata taaatcaaat aatagagaaa tataaaaaac
    14401 agttggattt atatgcagat attattgaaa ctttaactgg taagtcagta aaagagaaat
    14461 gtatatattt atttggagtt gatgaagctg tttgttatta gtttttatat ataaattaaa
    14521 cctaaaagat agaggaaggg gaaaaaatga gatttattca tacatcagat tggcatctag
    14581 ggaagtccct agaaggccat tctagaattg aggaacaagc aaagttttgt gaggaattta
    14641 taaaaatagt agaatcaaat gaaatagata tggttataat tgctggagat gtatatgata
    14701 cttctaatcc cccagcacaa gcagaaaaac tattttatca gactgtatca agacttgcca
    14761 ataatggtca gaggtgtgta ttaataattt caggaaatca tgataatcca gagagacttt
    14821 ctgcaataac acctctagca catgaacagg ggatacttat atatggttat ccattaagtg
    14881 ctacgataga agctaagtat aaagggtttg aaataacata tgcaactcag ggatgtacaa
    14941 aactgaatat aaatggagaa aatattgtga ttgctacatt accttatcca agtgaaaaaa
    15001 gattaaatga agttttttca agtgaagatg agtttgagaa acaaaaaaat tattctgaaa
    15061 aggtaggaga tatatttaga agtttagaag aaaattttag aagtgatact ataaatatag
    15121 ccgtatcaca tatatttgta ataggtggag agagtactga atcagaaaga cctatacaat
    15181 taggtggaag ttttttagtt gaaagaaaag atttgccaga aaaggctcaa tatactgcac
    15241 taggtcattt acataaacaa caaaaagctt ccgaacgttt aaatgcatac tattctggtt
    15301 ctccacttca atatagcaag gatgaaagag cgtataccaa aggggcatac atagtggata
    15361 taaaagctgg agaaaaacca ataattgaag atgtatattt taataattac aaacctatag
    15421 aagtatttaa atgtaatgga atagaagaag cactagatat atgtgagcaa aatcaagata
    15481 gagagatttg gtcatatttt gaaataaata ctgatgagat aatatcacag aatgaaatta
    15541 aaaaaatgaa ggaactatta aaagacataa tagaaataaa accaataata acatcttgtt
    15601 atgaacaaga aagtgtagat ataaaagaaa agtctatggc agagttgttt agagagttct
    15661 attcttttag taaaggtgta gagccaaaag gagaacttat ggatttattt ctagatataa
    15721 taagtgaaga aggtgaatca gcagatgaga ccaattagat tagaacttac aggacttaat
    15781 agctatattg ataaacaagt tatagacttt gaaaaattaa ttgaaagagg tttgtttggg
    15841 atatttggaa ctactggaag tggaaaatct acaatcttag atgcaataac tattgctatg
    15901 tatggaaata tatccagaaa tacaaaagaa tatataaaca gtgtgtgcga taaggcaatt
    15961 atatcatacg agtttgagat aggaagcaaa aacactagaa gaagatacat tgtagataga
    16021 accattgcta gaagcaaaac aggaacaaag acctctaatg ctagattaat agaagtattg
    16081 aatgacaata tccaaaatgt tttagctgat aaagttgtgg aagtaaatga aaaagtagca
    16141 caggttgtag gattaacggc aaatgatttt actaggtcag tagttttacc acaagacaaa
    16201 tttaatgagt ttctaagatt atcacgtgca gatagaagag acatgttaga gagaattttt
    16261 aatcttgaga aatatggaag aagtcttgga gaaaaagtga aaaagagaaa aaatatacag
    16321 ttacaaaatt taaaagattt gaaatcaaaa ttaagtcaat atgatggaat tacagaggaa
    16381 gtatacaaca atataaacca agaattaata gagttaaaaa atttggaaaa agataagaat
    16441 aatgctttag acctagctca aaaatcttat gaagaaagta aaacagtata tgaagaacaa
    16501 ttaaagttag aaaaaaatga acttagaaaa aatgagcttg atttaaaaaa tagtgagata
    16561 aaagaaaaga ttgctcttgt agaaaatgat agtaacgcta aaaaggtaga cccatacata
    16621 agttcagttc aaaatctaga aaaaaagata gatgaagata gctttgtagt aagtaattta
    16681 gaaaagaaat tagcaatatt aaatcaggag cttgaagtta caaaaaatag atatgaaaaa
    16741 ataaacaaaa ttaagaatga agaagtccct aaattgagtg aagaaaaaat aagattgcaa
    16801 caagcaataa aattagaaga agagttagtt ttattagata gagagttaaa agatctaaaa
    16861 gaaagtggaa ttaatttaaa taagactaaa gttgaattag aaaaagtaaa gcaggtgtca
    16921 gagtccagaa aagatgcagt tactaaatct ataaaagaag ttgaaggaaa gattgataaa
    16981 gtaaatataa gtgcagaatt aaaacaaaaa atatttctag catatgaata cgagaaagat
    17041 tacaacaacg tattggaaga aaaaaatcaa aagctaaata aactagaaga aatactcaaa
    17101 gagacagaaa atataaattt aaaagttaga tatattgata aagataagaa tgatgtaaat
    17161 cgtaatttag aaaatttatc acttcacttg gatgttcttc ttaaaaaatg tcctggtaag
    17221 agtgctgatt tacttttaaa atctgaatat gttactgaat taagaagtaa agcaaataat
    17281 actaaagaaa atgaaattaa gaaaagcagt atacaagatg aattaaagat aatacttgaa
    17341 agtaagttta atactgaaag ggaagtaaat ttactaaatg aaaaacttga aaataataga
    17401 aagaatagag atgacctaga aaaagaatta gaagaactaa agtatttaaa tttagcctct
    17461 gaacttagac gtgaattaaa agaaaatatg ccttgtccag tttgtgggtc aaaacatcat
    17521 gaaaatcata atataactaa gtatgatgaa aatatatctt ttgtcaaaga aaaactggag
    17581 aaattagaaa aagaaaaaat aagtataaga aacaatatag aggaattaaa tgctaaagta
    17641 agtgggtatt tatctataga aaaaatgaag actaaagaac ttgaagatgt aaaaggaaaa
    17701 ctagggggga ttccatctag tcaattatta aaaaaacttg atgaagaaca aagaaaactt
    17761 gcattgttaa aatctaatat tcaggagtgg gagaaagaaa aagagagtac tgaaaataaa
    17821 gtaacattag ctaaagaaga aaaaaataaa attgaaaaag aagaattaaa aataagagag
    17881 agtctgaata attataagaa attaacaaga gatttaaaca tagaaataga aggtttagaa
    17941 aataaatgta aaaaacttaa acaggaatac ctaggattaa agacgattac taaagtctct
    18001 aatctattgt caaaagtaaa agaaataagg gagaatgaaa aagaattaga attactaagt
    18061 actgggcatt caaatttatt aaaaaataga gactcactag atattaaaat aagagaaaat
    18121 gagagtaaac tacatgaaat agagatagaa ttaataaagg ctagagaatt atatgtagaa
    18181 aagaaattga gtagagataa taagtataaa gaagttatta acataacgaa aggtgattta
    18241 gctaaaaatc ttttacataa tgttgaagaa aatatatgta agatattaga acaagaagaa
    18301 tcaagtaaga aaaaattgga agagcaaaga ttagaatatg aaaaaaacat tgctgaaaag
    18361 cacaatatag atggtagact taaaactgct aaggaacaat ataaagacca aaaatcaaca
    18421 ctaaataaac tattagcaga aaataaattt gaaagtatat atgcagtaaa aagagcttta
    18481 ttagatactg atgcagtaaa aaaattgatt gaagaaatac tagagcatga agaagaacaa
    18541 aagttattat catttaagat aaaatcatca aaagaaaaat taaatggaag aagcataaag
    18601 aaggagtact ttgaacagtt aaaagatgaa atttataatt tgaaagtaga aataggaaat
    18661 atatccaaag atattggagc taatcaaaat cagttaataa cactaaaaga ctctttagat
    18721 aagataaatg attttaataa acaacttaag gtagttgaac acaatgtgga cttattggaa
    18781 gaattagata aatgtgtgca aggaaataaa tttgtagaat atgtttctac aaaccaatta
    18841 aaatatatag cattggaagc atcaaaacga ttggatggaa taacaaaagg aagatatgca
    18901 cttgaaattg actcaacact aaactttgta atgagagata actttaatgg aggagaaaga
    18961 agaagcattg atactctatc tggtggagag acattcttga catctctatc attagcactt
    19021 gctctttcat ctcaaataca attaaaggga agtgcaccat tagaattttt ctttttagat
    19081 gaaggttttg gttcattaga tagtgaactt ttagaaattg ttatagaatc cttggaaaga
    19141 ttgcatagta ataatctaag tgtgggtata ataagtcatg tcgaggaact taaaaataga
    19201 gttcctgtta agctattagt ttcatctagt gaggctggaa taggttcaaa agtaaaaatt
    19261 gaatatagtt agaaaatata tggtaattga atatattatc taatatttcc aaataatatg
    19321 taattgtgat ataataaaaa ctaatattaa agttatttat aattaatatt aatataaaaa
    19381 catgtaagta ggagtgtggt aagtgttgga atcgcccaat aatttgattc agattatttt
    19441 tttaatagta ttacttatag ggtctgcatt tttctcagca tctgaaacgg ccttaatgtc
    19501 gttgagtaaa atcagaatca gatatatgca agatgaagga gttaaaggag ctaagttagt
    19561 aagttcatta atagaaaatc caaataagct tttaagttct atattggttg gaaataacgt
    19621 cgtaaatata gcagcaacat ccatatcaac atctttgttt ataggattaa tgggagaaaa
    19681 aggagtggca cttgctacag ctgttatgac agtattagta ttaatatttg gtgagattac
    19741 tccaaaaaca atagcagcaa ataattcaga aaaggtttct ctattagtat ctaaacctat
    19801 aaaagcaatt atatttatat taagaccaat agtatggata tttaatataa ttacaaatat
    19861 tatatttaaa ttatttggta taacaaataa aggagctaaa tcattcataa ctgaagaaga
    19921 gctaaaaact atggtaaatg taagtcatga agaaggcgtc cttgaaatgg aagagagaga
    19981 aatcataaat aatgtttttg aatttggaga tatgcaagct aaaaatgcaa tggtacaaag
    20041 aatagatatg gtagctatag atatggaaga tagttatgat gagataatac aggtatttaa
    20101 aactgaaaaa ttaagtagaa tgcctgtgta tgaagaaact atagatgata ttgtaggaat
    20161 acttaatata aaagatatta tatttttatc tgatgaagaa atagagtcat ttgatattaa
    20221 aaattatatg agagaacctt ttttcacgta tgagttcaaa aagataacac aacttcttga
    20281 agagatgaaa cttgaaaaaa gtcagatggc tatagttgta gatgagtatg gtggaacatc
    20341 tggactttta actatagaag atttagtaga agtcattgtt ggagatatag aggatgaata
    20401 tgatgaagaa gaggatgaaa tacaagttat aaaagaagat gagtatattg tagatggaag
    20461 tactaaaatt ggtgatgtta atgaacttat tggtgtaaac ttggaatctg aggagtttga
    20521 ttccatagga ggttttataa ttggacacct aagcagattg ccagaagaaa atgaagtaat
    20581 tgaagttgat aatataagat tttgtataga aagtatagaa aaaaatagaa ttaaaaaaat
    20641 aagaatatat acataatgta ataatgttct taaatttatt aga
//
LOCUS       DAEPLF010000048        20422 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEPLF010000048 DAEPLF010000000
VERSION     DAEPLF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.048"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            619..1893
                     /locus_tag="KR314_001555"
     CDS             619..1893
                     /locus_tag="KR314_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBH0327945.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSAIYVKNATEGINF
                     LAKFFKDENPDKFIITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(2100..2417)
                     /gene="trxA"
                     /locus_tag="KR314_001556"
     CDS             complement(2100..2417)
                     /gene="trxA"
                     /locus_tag="KR314_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBH0327946.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2684..3436
                     /locus_tag="KR314_001557"
     CDS             2684..3436
                     /locus_tag="KR314_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBH0327947.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKN
                     KSVEYVLNIKGDKNILKKALEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHTHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3423..4304
                     /locus_tag="KR314_001558"
     CDS             3423..4304
                     /locus_tag="KR314_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBH0327948.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGRLNTNKSIFYQETK
                     AGVLSVELKEINNENIFMMEQNTPEFENLDNYRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4530..5885)
                     /locus_tag="KR314_001559"
     CDS             complement(4530..5885)
                     /locus_tag="KR314_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBH0327949.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKVEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
     gene            complement(7793..8302)
                     /locus_tag="KR314_001560"
     CDS             complement(7793..8302)
                     /locus_tag="KR314_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBH0327950.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLSEEFATILDISPNEVS
                     SYISSHIPQ"
     gene            complement(8513..9922)
                     /locus_tag="KR314_001561"
     CDS             complement(8513..9922)
                     /locus_tag="KR314_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327951.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDMDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDVEIDYSP
                     ERTHDIWDESNEIDKNTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            complement(9926..11056)
                     /locus_tag="KR314_001562"
     CDS             complement(9926..11056)
                     /locus_tag="KR314_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0327952.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLRESIDEPIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEGFVKSYFESYSLIRG"
     gene            complement(11068..11871)
                     /locus_tag="KR314_001563"
     CDS             complement(11068..11871)
                     /locus_tag="KR314_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327953.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            complement(12253..13134)
                     /locus_tag="KR314_001564"
     CDS             complement(12253..13134)
                     /locus_tag="KR314_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBH0327954.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSNKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            13590..14564
                     /locus_tag="KR314_001565"
     CDS             13590..14564
                     /locus_tag="KR314_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327955.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYKLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNHPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(14680..15711)
                     /locus_tag="KR314_001566"
     CDS             complement(14680..15711)
                     /locus_tag="KR314_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBH0327956.1"
                     /translation="MKKIRKLATKLLITTIVLVSGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNNKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPTVGAVAQTQKPALPGKHTMLPHQDGAK
                     VVGKFGDIKDKHVMYEGVRCVVINSSVNTVTFLGSKSNLDPSNLTIAVIGFMNTPYGM
                     FALAPEGEPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIEITDEKANIGL
                     GQIMYIRYE"
     gene            complement(16323..17174)
                     /locus_tag="KR314_001567"
     CDS             complement(16323..17174)
                     /locus_tag="KR314_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0327957.1"
                     /translation="MSCILKLKQIFEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(17191..18111)
                     /locus_tag="KR314_001568"
     CDS             complement(17191..18111)
                     /locus_tag="KR314_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBH0327958.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDGIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(18221..19135)
                     /gene="murQ"
                     /locus_tag="KR314_001569"
     CDS             complement(18221..19135)
                     /gene="murQ"
                     /locus_tag="KR314_001569"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBH0327959.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNRVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(19234..20331)
                     /locus_tag="KR314_001570"
     CDS             complement(19234..20331)
                     /locus_tag="KR314_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBH0327960.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEKALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFDFKPSTK"
BASE COUNT         6963 a         3328 c         2213 g         7918 t
ORIGIN      
        1 cagtattctc aagtatttta acttaattat atatatattt attgaaattc agattatagc
       61 tttgtactaa tcttttctca aatatataca ttggtaatat aatttttaat ttatataaat
      121 taaattaaat ttttttataa ttttttttat aaaattattt gtattatatt ttgtattttt
      181 tataaaatta ttatacttgt acatctttta taacatataa aatctttttt tctaattata
      241 tacaatatat tttccattat gttctaatca atttctttac tatttttaca actttattag
      301 tcatattcat aattatttct aattagtgtt taacatttta tgtaaaattt ataaaaaaat
      361 gtatataaat taaaataagt gactatacta aaaatatgat acggataaac aataagttca
      421 acctatctcc cccaaaatca agtgagctac agtttccccc aactgtagct cattttcgtg
      481 taatttaata aacatctcaa caagaattaa ttaacaaaac cccttataaa aacataacat
      541 atattaaaca agctcttgat taaaacttga gcttttttaa taaaaaacag aatagtttat
      601 tagataaggg gtttttttat gcaattcatg gatgtatcta aatacattga aggtgcaaac
      661 cttaaagtta aaactaaaaa tggtgaaact gttaaaagaa tatattttaa caactctgcc
      721 actccattag ttttaaaaaa cgtagtggat aacttaaact gtgagattcc ttggcttact
      781 tatattaatg ctccaggaat aatctcagaa aaaaatactt taaaatatga aaatgtacgc
      841 tctactatcc tcaaacttat tgatggagac gaagaaaagg attctgctat ttatgtcaaa
      901 aatgctacag aaggaataaa cttcttagca aaatttttta aagatgaaaa tccagataaa
      961 tttattatta ccacagcaat ggagcatatg gctaattacc taccttttaa agttaatttt
     1021 cctacaaaag ttgttggaat tacagaaaaa ggagaacttg atttatgtca attagagaat
     1081 atacttaaaa attatgctgg taatgtatct ctcgttacag ttacaggagc tagtaatgtc
     1141 acaggtataa ccactcctat atatgaaata gcaagaatag ctcataagta tggtgcaaag
     1201 atattggtgg atattgtgca agtaattcag cataaacctt tttcaatgat gccacatgaa
     1261 tgtgatgagc atatagattt tgttgttttt agtgctcata aatgttattc tccactaaat
     1321 ggaggagctc tggtaggacc taaaaaattc tttgaaaaat ttaatcctgt actatatggc
     1381 tcaggttcta ccaaatttgt aaatgataac aaaatactat atgcaaacgt accccaacgt
     1441 tttgaagcag gatacccaga gtattttgga actcttgcaa tgggaaaagc actgaacact
     1501 ttaaataaaa ttggtctaaa cagaatttca aaatacgaaa gagaattatt tttatatgct
     1561 aaagaagaaa tgagtaaaat acctaatata ataatatatg gagataaatc aaataatgta
     1621 attatcccaa atatatcttt taatataagt aatatgtatt acaaagatgt agcaaaatat
     1681 ttagttgata actttggaat agaaactgga gctggagtag taggtgcaga tatttatgta
     1741 caaaaattat taggtataag tccaaaagaa gcatacgtca gatttatgag ggaaaatcct
     1801 gtaggtcttg ttaggataag cttaggaatg tacaatactt ttgatgaggt ggatagcttt
     1861 atacatgcta ttaaaacttt ggcttttaaa taagcttttt ctattctttt ttatcaataa
     1921 atagagagta gttttgtttt cacaaaaaaa caaattatat caacagtcaa tttcattagc
     1981 aattagacac tcatttgtaa ctatacaaac tattttacac tatacaaata aaaaactcat
     2041 tacaatgagt ttttttgttt atatatgata tttattttaa ttacttcaat ttactttaat
     2101 tacatatatg attcaatttt gttttttaaa ttctccttag gcataaaacc tattaatcta
     2161 tctacaggtt ttccatcctt aaatattagc atagttggta ctgtagatac ttcaaatttc
     2221 tgagctaatt ctatactttg gtctatatcc accttcaaga agtttgcttt ttcaaccatc
     2281 tcatttccta aagcttcata tattggtgat agcatcttac aaggtccaca ccaagtcgca
     2341 aagaaatcta caactactaa accatcttta ttttctactt catttataaa ttcattatta
     2401 tttattattt ttaccataat atacatctcc tttattatct tcttgatact aatatactac
     2461 gccctatata acagttcaat taggcacttt tttgtaaccg cctattttta atagatttaa
     2521 tattatgata aatagattta taagattaaa tatttataag actaaatatt tctgatatat
     2581 tttatttttt tcttaatata gttgacattt tctcccttaa attataaaat caaattaata
     2641 actacatagg tagttactaa tcttttatca aaggagtttc tatatggata atataattaa
     2701 tgaattacaa aatataggat ttactaaata cgaatctcaa atatacatat cattattaaa
     2761 ggaaagccct ttaacaggat atgaaataag taaattatca ggtgtaccac aatcaaaagt
     2821 ctatgaaaat ataacaaagc tattaaacaa taatataatt atcaatgttg gaactgaccc
     2881 tattaagtac atacccataa accctaatga attattaaaa aataagaaaa aagaatttga
     2941 taattctatg gataatctaa agaatagctt acagatatta aataaaaata aaagtgtaga
     3001 atacgtttta aacataaaag gagataaaaa tatattaaaa aaagcattag aaatgataaa
     3061 aaatgcaaaa caagaaatta taatttcttg cccttatgaa gaaattcttg aattaaaaaa
     3121 agaactccta attgcttaca aaaaaaatat taatattgaa atactacttt tagaaaacaa
     3181 aagtctgaaa ggtcttgata atattcatac tcatggaggt aataaaacta cacttgaaaa
     3241 taaaatgctt tacaaaggaa ttattttgat tgtagataat gatgaaatat tgacaggaaa
     3301 cttatcagaa ggcagtgagg ccatttcaat ttggacaaaa aactctaaca ttttatatat
     3361 tggagttcaa tacataaaac atgaaattta tatttcagaa caaataaaag gaggttataa
     3421 aaatgaacaa aattaagatt aaacaagttg atgcttttac aactatacct tttggtggaa
     3481 atcctgctgg tgtcgttact gatgcctcta acatttctga tgaaattaag cagaatgtgg
     3541 ctaaagaaat gaatttatca gaaactgcat ttgtttcaga gtcaaatgtt gcagatttta
     3601 aggtacaatt tttcacacca ggtttagaag ttgatttatg tggtcatgca acaataggta
     3661 ctttccatgc acttttggaa gaaggaaggc ttaatactaa taagagtata ttttatcaag
     3721 aaacaaaagc tggtgtatta tctgtagagc ttaaggaaat aaataatgaa aatatattta
     3781 tgatggaaca gaatacacct gaatttgaaa atttagataa ttacagaaaa gaacttgcac
     3841 aaatgatagg tgtgcatgaa gatagacttc ttgaatatcc tattataaag gtaaatactg
     3901 gactttggtg gcttgtattt ggtctaaaat ctttagatga tttaaaaaat atctctccag
     3961 atttaaataa aatcaaaaaa tttagtggtg ataataatat aattggtata actccatttt
     4021 gtatagaaac tatagataaa aagtgtgact accatttaag atctatagca ccatatgttg
     4081 gagttacaga agaccctgta tgtggaactg ggaacgcttg cgtatcatct tatatagtac
     4141 atcacaatat tttagataaa actagtttta ttggtgagca aggtaacttt ataaacagac
     4201 ctggaaaggt atatgtaaat ataactagag aaaacaatga cataacttct gtaaaaattg
     4261 gaggaagtgc ttatactgtt ttaagtggtg aaattatgta ttaatcccat acctaataaa
     4321 aacaaataaa aatactagct aactaaagct agtattttta tttgttattt ttatttaata
     4381 atcacactct aacaaatatt ataattatat atatcataat attgtattta gaagtaaaat
     4441 tttattttta ttattaatta gttataaatc aatctaatac aacataatta cagtagatta
     4501 atctataaaa cataataatt acttactgat tatttatcat cctcaacaag tctacttaat
     4561 gtcttatcag ataagaataa taatatcgct gcaacaaata gtattactgg aattatagta
     4621 tatactttaa tcatatcaaa tttaagagta aatgcatata tatatccata tcctttacca
     4681 gctataaaag tagcaaatgt ccatactccc ataagaacac ctagaagctt cttaggtgca
     4741 tacttactta caaaagagtt accaagtggt gagaataaca tttcaccaag acttaaaaga
     4801 ataccaaaca tgattatcca tagtatacta gctttagcag tttcaggagc tccaactcct
     4861 cttgaaaatt ctgcaccaac aagcattaag aatgcaagac ctaagaatat taatccaaga
     4921 cctgtctttt taaataaact catatctcct tgtggtctac tagcaagttt aaaccataat
     4981 gctccaagaa ctggtcctaa tactatacat acaagtgaat ttaatgagtc aaaccaagca
     5041 agaggaacat caaatcctcc aacattcatg tttacaaatg ctgcaccata atcataaact
     5101 gcaagataag ttagatacca gaatacccag aaaattacag agaagaatga tataagtata
     5161 atagcccaaa ttctcttctt atccatgctt gtaagaggag acttatcttt ttcttcaact
     5221 ttttcagcaa ctacaccttc tttaaaaggt ctctttcctg catttccaag gaatctccat
     5281 ccatatatga accatattgc acctattaca caaagtacag ctgctataaa gaaacattga
     5341 gaaaatccaa gtacaccatt ttttgcaaat gttttaaggt aaagaatacc aactgctata
     5401 gtaccaataa aagaacctat attaacaaaa gaatattgta ctgaaaatgc tgtatcaagt
     5461 tcttcttgac tatcaaaaag ctgtccattt accgcagata cgttaccttt aaagaatgca
     5521 gtaccaatcg aaactaagat tatcattgca tttaccatag aaactgtagt tgcaatagaa
     5581 ccaagatagt aacctacccc catgataagc atacctatag gtatagtata tctagctcct
     5641 atccatctat ctgatatata tcccccaatt ataggtgcaa ggtatgtgaa tgcaacaagg
     5701 ttggcttgca ttatagcagc ttgtccttta tctattccaa gaccaccatg aactatagct
     5761 gttgtaagga atatatatat taagtacttt gaagcataat atgctgctct ttcaaatgag
     5821 aaagttgttg agcagatata aaatcccata ggaaatttac ccttcttttt tactgttgag
     5881 ctcataattt taaatccttt cttaattgta tttttatttt ttacgttttg tccaaattct
     5941 ttcttttatg aaagatataa tattattatt ttgtaaaaca atttgtaatt attattaaga
     6001 taattgcgca tttatttgta gagtacatat tctcatggaa taaaattaat acacttctta
     6061 atcataaatt taaattatat aaattcacta ttttcagata tagttataaa ttaatatatt
     6121 ctcatctaat accatgaaaa aaccttcgcc atatgcaatt cttatttttt acgtgatagt
     6181 attttacaaa ctaaaatccc ccttttgtag caacttctgt aattacattt tagtgctttt
     6241 ttaaaggagt ctcgaataaa gcaacatata ttttgttcaa ataatgtttt tatttacttt
     6301 ttaaatttat atattacttc actcaaccaa ttctcaactt aaataaataa actactatct
     6361 attcacagat tagacctatt tcttacacat tagtttataa taaacactgt gaatctaaac
     6421 ataaaaactg ttattttaaa gtaattaccc atatattcgc tttgatacta tttattgagg
     6481 ttatactctt tatttataat atattattta taaaatacct caaaatatat tattaataaa
     6541 atacctcaaa ctgaaaactt tttcacgaat atttattttt cacctcccct tttttatttt
     6601 accatgtaaa tcgtaccata aatatgcact tatatgcaag aaaaaaatct attaatcgac
     6661 agtaaataat tagaaaaatt taaattatat ttgttaagtg tattatataa aatatttcct
     6721 atgaaaatat aaattaaatt aatgttcaga atagttttta gatatgaaat cttgattttt
     6781 ttattacttt acttatttgc tcaaatatat ttttgtatgc agtatataat aatcaaatta
     6841 tacacttctt ataactgcgt tgatttgaaa ataatattaa tagttaatag aagaaaaaat
     6901 ttaaattaat ttttaaaaaa tctattaagt taattgaata cttttttatg tatttaatta
     6961 attttataaa ttcaattatt ttctaaattt aggtatgctt tactatataa aaatgatttt
     7021 ttatcttaat tttctaaata taagtgaatg ctatcatttt aagtatctta taagcaatgt
     7081 aactatatat ctctaataac tactttttta acatgcttaa actatttata agctattata
     7141 tttatgtatt aacctaataa ctttttcact aaaaaataac aatctattca aagataaact
     7201 atagtacttc ttctactttg tttcaataaa atactaaata aatttcgtac atccacaatt
     7261 tttcaggcta cactataaat atacaatatt tatcttagtt ataagtttaa gaatattttt
     7321 agtcttattt tgtcgaatat atttttaata aaacttcata attctaagca tatttttaca
     7381 ctgtgttttc atgtacaaaa ataatgctta caaataaaca acataaataa aacttaaata
     7441 agatggattc tattcaacat agtatccatc ttattcattc aaaacctaaa aggatttttt
     7501 ataatattat atattttaaa atctaagtaa ctcattatag tatcaatttc acttaaagct
     7561 tacaatacac ttattaatct tgctttatat taaaaacatc tacaataaca atttaaatct
     7621 ttaagtgata tccatctcac acctaaagga gtgggctctc cgcactattc tgtaattctt
     7681 caatacaatc attcttaatt ttttatataa tttaattata tattttattt taccaaaacc
     7741 agcatacata aataatactt aaaatagatt aaataaagga tactaaaaca ctttattgag
     7801 gtatatgact tgaaatatat gaactaactt cattaggaga aatatctaaa atagttgcaa
     7861 attcttcact taataacctt tctgcttctt tcattatatt atcatctgct tgatgtgttt
     7921 ttttaccaat agattttaca tactctctgt tattatctat acttcttact aattttatta
     7981 actcttcaca tttaccacct ctgagtattt ttttaaattg ctcacttctt aatttttcat
     8041 tatctatcca tgatgtatcc atattaggaa tatcatttat taatgaaagc acattaattt
     8101 tagaaagtaa ctttctcatt ggtacttttt cattatctac aggaattttt attactgtat
     8161 tattagaata aataggactc aatacataat actctttttt tattccattt gtaaatcgtt
     8221 catttgttat atccatgact ttgcagactc ctgtcatacc atacattata taatcatcaa
     8281 tcttaaacaa aaacatcacc atccttaata aaaaaaatag ttttgacaac taaatccatc
     8341 ttgccaaaac tatctgctat atattatatt ttaacacatt ttaagactta aatgtaagta
     8401 aaacttatat tattaaaatt aaaaaataca ttaaccattg attaatgtat tttttagatt
     8461 tttataacaa gcatatcaaa ataaattaat acagcttatc tcctgtgaat acctataaaa
     8521 ttggtgcttg gctattcatt gagtacccat catttcttac atcatttatt tctaatattt
     8581 catcatttgt tttaacctta gtaagttttt taactgctcc ataaggttct tttaatgaaa
     8641 ttttatctcc tcttccagaa ataacccata aaattttata ccctctaggt attacttgta
     8701 gtttattttc acctttacca tcagtaaagt agactaacaa attaatcttt ttattattag
     8761 catactcaaa gactggagaa aatctagtac ctccacctat cctaattcta tcttttatat
     8821 ctttttcagt tctcacttta tatacacgtc taatttcatt atcacattct ataattgtaa
     8881 tttcatgatt atagtttttc actatactaa gaacttcttt aatagcttgt ttaaattctt
     8941 catcactaat acttccactt acatcaagtg caactgctat ttctgcctta tgacttctaa
     9001 gttctcccct caaatctaaa cgatttggtt gtcttctatt tcttcttgtt atagtcttct
     9061 ttttattatt ttcaactgtt cccattattc tattaagata taaattccat ggcaattcac
     9121 ccttactatt tttaagtgat gatatcatac tttctaaata agcaggaact ttaccttttt
     9181 gagaactgtt aataaatttt tcagtaaatt ctctaagggt atttttatct atctcattgg
     9241 attcatccca tatatcatga gttctttcgg ggctataatc aatttctaca tctccatatt
     9301 tatcactatc atcttcctca ccatcttcat ccacttccat caaatctaat tcagtctgaa
     9361 tcttttctac ataatattca aaaggttcat atggttcaag ttttaaggaa tattttaaat
     9421 ttacccactc taaggtagtt gcatatggtg gtaaattgtt tagaaattta tttactacta
     9481 tatccattgc catattaatt gccaatgtac tatactttcc ttttaactcc tttgctctca
     9541 tcaaatgcat agataatata tgatgaattt catgtttaat tgtagtctcc atttgcttta
     9601 tgtcaagcgt taaaaagatt attggattaa aatatataac atatttagct cccttaaaat
     9661 tcacgcctgt aggactactt atatcaaatc ttatttctct tgacatctga aacaaaaaat
     9721 atccataaaa attatcttct tcttccataa gacttaaatt cactttatct acaagactaa
     9781 aaaactcata cttaaaatct tgtggtatat ccatatcaaa tttctcatta gcacgatttg
     9841 cctttagcat agaatgagta tttataattt cacttgcttt attataaagc tcttttgctt
     9901 gtttatcaaa ataattttcc atacattatc cccttattaa gctataagat tcaaaatatg
     9961 attttacaaa accttcattt tctatggctt ttttatatac ttcaatataa ctgtttctaa
    10021 tatccttcat aatacctatc attaaatcta caggatacat ttttaaaaat tcaataaatc
    10081 tattaatata gtggtttgat tcatatttat cattcttaat atttaattct aagtctttta
    10141 gaatgttcat agctgataga taaagtcttg tatgactctc attttttact ctttctacaa
    10201 tgggctcatc aatagattct cttaaaaaaa catcttcata ggatattaat ggttcagagt
    10261 ttgattcaat aaaacttata aattcttctg caataacttt acctacatta ccctttacaa
    10321 catttaaaaa tactgctcta ggaatagaat tatttttttc tttataaact ttataaattt
    10381 tagatactct ctcataactt ctaggagtag ctcttacatc atcttcattt attttatgca
    10441 gatattctgg aaaagtggaa ataaactcga taacttttct ttcaataccc tcatctattg
    10501 cccattttat ccattgtgta tggtcactct ccatatttaa ccaaacaaac ctattttctt
    10561 gggctgcatc catatctacc acttgataat caaaatctga accatattta cttgatggat
    10621 tcattgctgc taaaatcttt acatcatcat gcagtttata tccattaatt tctctattta
    10681 aaattaaatt cataagttct tgctgtactg tatgctcaca acgatttatc tcatctataa
    10741 ataaaagaac tgtcttactt ttagatatct cttcatcaat ttctcttaat ttgttatgaa
    10801 cagcatacac tgttgttttc ttttcagtct tatatccatt agaattaact cctacataag
    10861 attctatagt tggaagacca cctatttcac cctctttaag taagtttcca tcaataacaa
    10921 ttaaactcca atcattttct ttggcaagct tatttgctaa agctgttttt cctattccac
    10981 tttcacctac tattaaaggt acttctccag tggataatat taagtcaacg ctttttaatg
    11041 tgtctataaa attcattttt ctctctccta cataatcttt aatttctcat ctacagctat
    11101 tttttttgct gtcttacttc catatttata aataaacata attttatcta ttggtatact
    11161 ttcgctattt ctatctataa cactacaatt caaaaaatct ctaatatact tttcctcaac
    11221 atcttctttg gataatactt cttttaatac actctctaat tcatctttag atactttttt
    11281 cagaatgtac ttaatcacta aaatattcac ttttaaaaat tccaacattt tatttttatc
    11341 taaatcagtt ataaagctaa tagctgcttc attatttttt atagctataa gttctgcatt
    11401 ataactaggt gaatctatat atctcaaggc atcataactt attcttactg cctcctcttg
    11461 tacactttca taaggttgtt ttataaactt tatcgaatca taattttttc ttactgctaa
    11521 cagttgtaat tcttcacttg gatttttagc atatttaatt gcccatcctg cttgatttat
    11581 agccatttca ataactttat cagttgggtt tttgatataa tccagtatac tccatccctc
    11641 atttactgct tttatcatca tatcttctgt tggattatct ataaatttaa ttgctctaat
    11701 attattatct attgctagtt cttgcatctc tctggttgga ttatttatat attctagtac
    11761 aagcccattt tctttaatag ctaacaattt catttcatca gttggattat ctattgtagc
    11821 tataatattg ggattatttt ttatcatttc aataactctc aatttttcca taatgtgcct
    11881 ttctttctat aattattttt atatctatat taattataat aatctattcc tatttatata
    11941 attgtttctt attataatta atatactttt taatcaaatt taaacagaat aattatacca
    12001 atatataaaa aaatttctaa aaaacaataa aattaagtta taaacaccaa tatatttcta
    12061 ttatttcaat aagtatacaa ttagattaga atctaacgct agtaataaaa aattactttc
    12121 ccaaaataga tataagcctc attttatcgt tcaaataatg aaaatatatt tgatttcatg
    12181 caaaaagagt gcctaatgaa ctaaaaagtt cattttgaga cactcatttc tttatataaa
    12241 tttatatagt ttttaatcgt tcgcattcaa ccactcagtt aagactttta tatatttctc
    12301 attttcttca acgaatggca tatgtcttga atactcaaat aactcccact tactattagg
    12361 tattctatca tacatagtct ttgcaatata tggcgaacat aaatctattg ctccactagt
    12421 tactagacaa ggctctttta tttcatgtaa tctatctgta aattcatatc cagaaagagt
    12481 tcctgtagga gaaaattcat tttgtcccca tcctacaata tatgcttcag aacctgattt
    12541 tttaggtctt ctcaggcact ctggagaatc ttctgtcact tcacctgcac aatacatttc
    12601 cataaatctt tctaatgcat cattatattc tttattagag taatctccag tatttactgc
    12661 atcaagcaat gctttttggt caacctcact catatatgat attcttcttt tttgttcttt
    12721 ctcccaaagt tttgcagaag aaagagtaga tgaaaggatg taagacttaa taccttttgg
    12781 tttatattca atagcatacc aaatagcctg catacctccc caagattgac ctaacagatg
    12841 aatttcatca agtcctagat gttttcttag ttcaataagc tcctctatcc aagtatctgc
    12901 attaaataac tcaggatgcc cttctacaaa ggaatttcca caaccaattt ggtcatacat
    12961 tattacttgt ctaccacttt cagctatctt atccaatacc tcaaaatagt tgtgtgtaga
    13021 acctggacct ccatgaagta atactaatgg cttcttccct tccgtatttt ctcctactat
    13081 acggtaatac gttttaaaac ctttaaaagg catatatcct tcagtaattt tcatatttat
    13141 ctcctccaat ttgtaattat aaacaataat caagttcatt aacaatacta tttaaaacct
    13201 ctcatacgta tttaattatg tcttttaatc tttataccta cttttataaa tagtataaca
    13261 taaaagttaa aaatctctat actaaaccaa ttataaaatt atattaacta tataatttta
    13321 ttttttaata atagtatata catatttgat tattttcatt tgctatttaa aatcaatttt
    13381 aagttttctt cttataataa acttcctctt tgtggtctca tgttagtctc taaatttaaa
    13441 attttacaaa aaattttcat aaaataaaat tatattgatt gtatctaaac aaatagggca
    13501 ataaaataat taagtatagt tacttacctt tcaagcaata ttagttttat gttagaattt
    13561 tattatattg atttacagga ggtttgcatt tgaataatat taattcactt aaagtaataa
    13621 aattatctct tggatgttct cttgctatat tcattgcttg gctattaaaa ttagaatatt
    13681 caatggttgc tggagttata gtactactta cagtaaagga cactaaaaga gaaactctta
    13741 aaggctccat tggaaagatt tatggttttc ttttatgtac tattttttca tatttatgtt
    13801 ttaatatttt aggatataat ttgacttcat tttcaatatt tatatttata attataccac
    13861 tttgtttttt gttaaatatt caagatgtta ttgctatgtg tgtagtcata gcatcccatt
    13921 actaccttca aggagaaact tctatcaaat ggattttaaa tgaagctgga atatttgcag
    13981 taggaactgg aataggtgtc ttaataaata tgtatattcc aacaaatata cataaaatcc
    14041 atgaagttca aaaaaagtta caggaagaag tgagtattgt acttatagac attgcagata
    14101 ttattgtaaa tcctaaaaaa gaaaatggat atagtagaga tttatataaa ttaaattcac
    14161 ttattgacag cagtatttct gaaacttacg ataatattaa taatacttta ttaagtgata
    14221 ctagattctt cttagaacat atggatatta taaaaagcca acgggatatc ttagaaaatt
    14281 tgtatagtta tgtatctcaa ctaaattcta caccaccaca agctcacatt ctatctgctt
    14341 tcatacataa aataggttat actgaatttg aagctgaaac aggcaatctt cttcttgaag
    14401 aattgaagag attaatgctt agtatgaaaa accatcctct acctgttgac agaacagagt
    14461 ttgaaaacag agctatttta ttcctatgct tgactgagtt aaaacaattt ttagttaatc
    14521 gtaaacatgc tcagatgctt agagataata atttttataa ataaaaaggt gagaaatcca
    14581 gcagttgacc tgactacaaa atatcagata taatattaaa tgcgccccta gtaagagggc
    14641 gcgatgttta taacttattc tactgtcatg attaaatcat tattcatatc gaatatacat
    14701 tatttgacca agtccaatat tagctttttc atcagtgatt tctattttaa tagtcacatc
    14761 atttttttta cagtactttg ctaattctgc accagaacga tttttaccac taaaatctaa
    14821 agaaaatggc ttatcatatt taaaaggata ttcaacaggt tcaccttctg gtgctaatgc
    14881 aaacatccca tatggtgtgt tcataaatcc tattacagca attgtaagat ttgatgggtc
    14941 taaatttgat ttactaccta gaaatgtaac tgtattcact gatgaattaa ttacaacaca
    15001 tctaacacct tcatacatta catgtttatc ctttatatct ccaaattttc caacaacctt
    15061 tgccccgtct tgatgtggta acatagtgtg cttcccaggt agtgctggtt tttgcgtttg
    15121 tgctactgct ccaactgttg gatatgctgt cttatcaaat ggtaatgggt ggctaagact
    15181 cttgtatatg atatcctttc ctgctgattt attagcatca atatctacat gatacaaacg
    15241 cccatcacca aaacgtataa gattccactg gtggtcattt gatgtgctgt gtacataata
    15301 agattcaatt cccatagcgt cacacaaagc tttcatgctc ctagccattc ctgaacatgc
    15361 acctttttta gtaaccaatg tataggcagt attcgctcct tctactgcat tccagtcaac
    15421 atatgttgtg ttatttttaa tgtgattata ggcaactaaa atcttagcgt ctgtagtcat
    15481 gttctttgta attttattgt tcataaaatc tgctgttgct tgtgctactg agtttagttg
    15541 ggcttgtgac agctttggac catatacgct attggttcca gtgacatagc tttttggtgt
    15601 ttttgctgtt acctctttgg ctgtcatacc ataaactgtc atactcatac ctgagactaa
    15661 aacaattgtt gtaatcaata acttggtagc taacttccta attttcttca tacttagtat
    15721 tctcctttat aaaatattgt tacttcctat tctctaccat taataaagtt catttgggat
    15781 ttaaaaaggt ggattgtcaa tatgtatgtt tcttcattta tacgttcgtg aaatatttaa
    15841 atttcatgga aaatctaata gatattttct agataatttg tatacaatac aactcgcaaa
    15901 tcagggtgtt tgctgttagt ttgagaaaat gatgatgatt aatatattcg gaatctttga
    15961 cctataataa ataccattgt cttcaatcat taataatggt aagagtaaag agaattatca
    16021 ataatacagt taaaatcaac tttgtataca tttcatacat tctctcccaa caacattata
    16081 cttcttataa tttgtatatt caatacgttt tctgggaaaa tataggatat atctataaaa
    16141 atataaaaaa ggagttgaac ttaaaatttg attaaaaaaa atcaatttta agacaactca
    16201 tttgtattat aaaaatgcta tatattatta ttaagtaagt caatatttta gatatttatc
    16261 tatttcttat tgacttacgc ttatagtcta acttatttgt ttagttgata tataacctag
    16321 ttttaagcat tattaatatt taaataatca agtatccttt ttgtttcttg tagtttctgt
    16381 tctgcctcat ttaagttttc ttgaattata gaaatatata ttatatctat caacgtaagc
    16441 attgctattc ttgaagaaat agctccttct ctaagccttt tttctatatt tggtactact
    16501 agctttaaat cactaatctt actaagatga ttgtcactat atttagttat acaaatgacc
    16561 ttacatcctt ttaattttgc attttctata gactttatta cttcttttga ttctccagag
    16621 taagatattg ctatagctat atcttcttta tctacatttg aagataccat aagttgtgta
    16681 tgactatcta aagaggtaaa tgcctgttta tttatcctta aaagtttcat ctgtaaatct
    16741 agtgcaacta aagccgaacc acctactcca aatatatata catttttagc ctttattatc
    16801 tgttcaactg cttcttttat tatatctttt tcattcaaca accttgtttg gttgatagtc
    16861 tctatgtttt tgtttgtaat tttgtttata acactatcta tattacaatc atagttttca
    16921 tctacatatg tgtattcatt ttctttgccc atcaaatctt tagctatctc aatttttaaa
    16981 tccccaaaac ccgaatatcc catttttcta gaaaacctaa ctatacttgc tgtacttgtt
    17041 tttgagtttg acgccaattc tgatacagaa agcttactta tagcctctgt attattaagt
    17101 atgtaatcag ctattttttt atctacttcc gttaaatctt caaatatctg ttttaatttt
    17161 aaaatgcaac tcatactcta ccacctctac ctatttacta taatatttct tcttagcaag
    17221 taaacatgct cctaaatctg gagtcaacac tggtctcact ataccaaatc tcgttttatt
    17281 caaatatttt ttaagtggct ctaatattaa atctcctgac ttaaacactc cacctgaata
    17341 agataccttt atctttgtac cttgttcaaa atgatgctct aatcctatta taagcctact
    17401 taattcataa gctgcatcat caaatatttt actagcagta ttatcacctt caataactgc
    17461 ttttaaacat atcgtagcaa actttgctat ttctattcta tctccttgta ttccatcatt
    17521 tacatatttt attatgtcat aatcattctc tatattaaga tgtcttttta ttaaatcata
    17581 taattttgtc tttgaacacc ttccatcaga ctgcattgtg aacattttta aggtagccat
    17641 tcctatataa tacgctgaac cttcatctcc taatacatag ccccatcctc cacatctgtg
    17701 aagattccca tctttatcta aagcctgtgc tattgaacca gtacctgcaa ttatatttat
    17761 acctttttcc ccatttaaag aacctgctaa tgctatttct acatcattac ctagagtata
    17821 atttatatga ctatatgcat tttttgttgc tacttctaat gcatacaata cttcttttat
    17881 ttttccatat ccagcaagtc caacaaatgc atacgttatt tcttctacat ttatcttagc
    17941 atctttacaa acttcttcca acccagttat taataatttc gttagattat caaatcctat
    18001 ttgattataa tgacaagttc ctttggttat ggttcctact atatttaatt cttcatctac
    18061 taatgtaaat gttgttttcg ttcctccacc atctacacct aaaaaataca tactatcctc
    18121 ctacaatatc tttaataatt ctttcaatac ttaatatcta aaaataacct tccaatgctg
    18181 tttttttaat attgaaaggt tatcagtact attatttatt ttacaatttt tctattgatt
    18241 ttaaagcttc acttatataa ccttcttgtc tatctaggat actctttgca ctatccttat
    18301 ctaaaccact ttttatcata aatatagcca atttaactct attatctgct tcttctaaga
    18361 atttttctgc aacgtcatat tttacatttg tagctatcat tattattctt tttgctcttt
    18421 caaccaactt ttcatttgat gtcttaacat ctaccatcaa gtttgagtat acttttccca
    18481 tcttaaccat agaagctgtt gttaacatat tacataccat tttttgagct gtaccacttt
    18541 tcattctagt tgaacccatt attacttctg cccctactat aatgtttatt ggtaaatccg
    18601 cttttttact catttcacta ttcggattca tagttattga tagtactgca caccccaatt
    18661 ccttagcata ctccattgca cctataacat aaggagtcct tcctgatgca gctataccac
    18721 atataacatc cttagatgta actcctctat ctattatatc ttgctttcca agttctttat
    18781 catcttcagc accttctatt gctttaaata ttgcttgatt tccaccagct attatagctt
    18841 gaacaagctc aaaatccact ccgtaagttg gaggacattc tgtggcatct agtattccta
    18901 tcctcccact ggtacctgct cctatataga ataatctacc tccctgcttc attctatcta
    18961 ctattatatt aacagctttt gctatactct ccttttgttc ttcaacagca taggctacag
    19021 ttttatcttc gttatttatt aattctatta tgcctaatgt atcttcttta tctatttgca
    19081 atgtattctt atttctacct tctgtaacta aattttctaa agtttttatt aacatatcat
    19141 ttcctcattt tcttaatatt aaatttttaa acacatttta actccaactt tcataattat
    19201 atttttataa taaattaata ttttcataat aaattatttt gttgagggtt taaaatcaaa
    19261 cttttgccat ggtttaatat aatctaatat aaataattct tcttttctta tatatcctac
    19321 tacattcgat ttaccactgt ttttcatatc agtcaaggct atttgtaact cccctgcata
    19381 atgaccatat tcactacttt caattactat atctcctctt tttattattt taggagtatt
    19441 aaatagttca aatttgtatc ctttatattt aacacgagat tgagtagacc ttatcagact
    19501 gtcagatata tcccctctat taaaatgtat ttcttcaaag agtatcttct tctcaacttc
    19561 tcgtatattt tcctctattg ctacatcaaa tgtaaccata tcttttctca tagaacctaa
    19621 tacctttagc tcctcatatg ttggataaca atttgatatt attatatcat ttatattatt
    19681 catagctatt atatgcttta cttgaatatc tataggtaga tttctgtgca tctcaattgt
    19741 tggaagccca tcagttacag gccatggtcc aaatgtggtt tcattttgac ttgttacaaa
    19801 aactgctgtt cttaatccat acttattaaa tctttctgta cattttataa agtgttctaa
    19861 atttaatcca ctatatctat gtggataaaa gttatgacat cctgtcaaat tttctttatt
    19921 tggttggtag tccattattg tatctatata attagtatta ttactcatat tgatttctat
    19981 tttaagattt tgaggattaa aagtcattaa ggccttttca gaacctgtaa atcccatatc
    20041 aagccttatt ccgtctgcct tcgtcttttt aaaaaaagtc aaatctttgt agcttatttc
    20101 taattcacca aatactctgg gatttacatc aagtattacc tcaaaacctt taaacttagc
    20161 gtattcattt atacttttaa attttttaat tatattttct ttagtatctt taactgataa
    20221 taaacatgaa aatatacgtg taaatccatt ttcagcagct ttatctatat aatcatatat
    20281 ttccttagat gttgtttttt ctggataaat tgatattcct aatcttccca tctgtgtcat
    20341 ctccttatat cctatatgtg tttaaattta tttcgaatta cacgaaactt ttttaaaaat
    20401 ttggtgaaaa aaagtttcat at
//
LOCUS       DAEPLF010000049        20188 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEPLF010000049 DAEPLF010000000
VERSION     DAEPLF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20188
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.049"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(125..1099)
                     /locus_tag="KR314_001571"
     CDS             complement(125..1099)
                     /locus_tag="KR314_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327961.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFEKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
     gene            complement(1294..1843)
                     /locus_tag="KR314_001572"
                     /pseudo
     CDS             complement(1294..1843)
                     /locus_tag="KR314_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
     gene            complement(2126..2605)
                     /locus_tag="KR314_001573"
     CDS             complement(2126..2605)
                     /locus_tag="KR314_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HBH0327962.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMVKELGVYLFGIDGLKLTV"
     gene            complement(2598..2735)
                     /locus_tag="KR314_001574"
     CDS             complement(2598..2735)
                     /locus_tag="KR314_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327963.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVEYCSRSCSKRARRSDLS
                     G"
     gene            complement(2759..4129)
                     /locus_tag="KR314_001575"
     CDS             complement(2759..4129)
                     /locus_tag="KR314_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0327964.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCTLTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKNGAENA
                     IHNKIKDICISLKAEDYLNMPRKIDNKIEIHLDSKILKYYKELEKEKILELDRDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEAIENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            complement(4078..4404)
                     /locus_tag="KR314_001576"
     CDS             complement(4078..4404)
                     /locus_tag="KR314_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBH0327965.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            complement(4686..7103)
                     /locus_tag="KR314_001577"
     CDS             complement(4686..7103)
                     /locus_tag="KR314_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327966.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIENFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDTGVKKVNKSIFNGQLDNERNQIWAEAVELCKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITENWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            complement(7115..9073)
                     /locus_tag="KR314_001578"
     CDS             complement(7115..9073)
                     /locus_tag="KR314_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HBH0327967.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTAISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWFAGEKWRLDVFNSHGKIYEASASHMFKVPIESIKKGDPLRQKGKISELAL
                     GYGGSIGALTSMGAIKMGLDEEELQPLVTTWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VKLQHGIKFIYNPGVLFIELPSGRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            complement(9073..9645)
                     /locus_tag="KR314_001579"
     CDS             complement(9073..9645)
                     /locus_tag="KR314_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HBH0327968.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            complement(9668..10240)
                     /locus_tag="KR314_001580"
     CDS             complement(9668..10240)
                     /locus_tag="KR314_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HBH0327969.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            complement(10293..10628)
                     /locus_tag="KR314_001581"
     CDS             complement(10293..10628)
                     /locus_tag="KR314_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327970.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     RLNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            complement(10643..11809)
                     /locus_tag="KR314_001582"
     CDS             complement(10643..11809)
                     /locus_tag="KR314_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HBH0327971.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPLTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            complement(11821..12258)
                     /locus_tag="KR314_001583"
     CDS             complement(11821..12258)
                     /locus_tag="KR314_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327972.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            complement(12329..12478)
                     /locus_tag="KR314_001584"
     CDS             complement(12329..12478)
                     /locus_tag="KR314_001584"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327973.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            complement(12459..12851)
                     /locus_tag="KR314_001585"
     CDS             complement(12459..12851)
                     /locus_tag="KR314_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0327974.1"
                     /translation="MDISESIAKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVEWGDEIAES"
     gene            complement(12882..13079)
                     /locus_tag="KR314_001586"
     CDS             complement(12882..13079)
                     /locus_tag="KR314_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0327975.1"
                     /translation="MLIGDNIKQMLRKRDIKPYKLAKELNIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            13155..13529
                     /locus_tag="KR314_001587"
     CDS             13155..13529
                     /locus_tag="KR314_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327976.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            complement(13539..13748)
                     /locus_tag="KR314_001588"
     CDS             complement(13539..13748)
                     /locus_tag="KR314_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327977.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(13795..14604)
                     /locus_tag="KR314_001589"
     CDS             complement(13795..14604)
                     /locus_tag="KR314_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBH0327978.1"
                     /translation="MNNLQIFKNKTFGEIRVIELNGEFWFVGKDIAEQLGYKDTSDAL
                     KRHVDDEDKGVGEIPTPGGNQNMKVINESGLYSLILSSKLPSAKLFKRWVTNEILPSI
                     RSTGTYNMIDLQTKLPTTYKEALQHLIEQVEVNEQLQLESKMKDQVIKELKPKADYTD
                     MILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHSET
                     IDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKGYSYQTSILG"
     gene            complement(14652..14855)
                     /locus_tag="KR314_001590"
     CDS             complement(14652..14855)
                     /locus_tag="KR314_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0327979.1"
                     /translation="MKKRSLKAGRIEAGYTQEELAYKIGIAKSTYNLKENGKRNFTEK
                     EMIMISYILNKTMDELFLEKRLT"
     gene            15032..15373
                     /locus_tag="KR314_001591"
     CDS             15032..15373
                     /locus_tag="KR314_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBH0327980.1"
                     /translation="MATFGERFKQLREEKKLTQDELVSKFNKVYFTSFNKSTISQYEN
                     NKRKPEINILENWADFFDVSIDYLLGRTLVRNHIDTVAAHRVNPHKDLPEEAQEQLND
                     YIEFLMNKYKK"
     gene            15385..15933
                     /locus_tag="KR314_001592"
     CDS             15385..15933
                     /locus_tag="KR314_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0327981.1"
                     /translation="MLILKKLRTEKGISLDKLSTDLNINKSTLSRIENGLREPKESFI
                     KDCSDYFGVSTDYLIGKINIDDSNKLTKCLNSTFPIRLKELRKKKELTQAELSKLLNC
                     SLSKIAMLETSKREPVKEDLLRISEFFNVSVDYLLGKTSIENYITTDEISKIIKSYES
                     LPKEAQEHINSYIEFLVDRYKK"
     gene            16009..16515
                     /locus_tag="KR314_001593"
     CDS             16009..16515
                     /locus_tag="KR314_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBH0327982.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            17135..18004
                     /locus_tag="KR314_001594"
     CDS             17135..18004
                     /locus_tag="KR314_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HBH0327983.1"
                     /translation="MIKNFDEWKEYDGFSYGSGRSEKIWLINEKTKDIGLFKFPKTKE
                     TGEYWAEKLACEIASLLKIPCANVEIGTYNKRIGSMSYLINKNDEKLIEGVWLINKYY
                     PCYNTDKLYDYKNNEYYSLKMIFKSIEEYNIKNDFLKMLIFDYLIGNRDRHQNNWALL
                     ESQNKITLSPLYDNGSSLCCYVNIENIDSFFTDHQRFEALINTRSRSRIRIDGKNKKE
                     PTHLEVLKYLNSNYYEQTIDFVEKIINLMNEVNINTLIDNYSENIISPKLKKLLKIYL
                     KEKVLRIKNIYNL"
     gene            18017..18724
                     /locus_tag="KR314_001595"
     CDS             18017..18724
                     /locus_tag="KR314_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBH0327984.1"
                     /translation="MSIKNGKDFLYVIWKDPKSRRQFIIGKLAKNGLYEFSYEYEIDE
                     AIKAGFNALISFDDISKTYYSEDLFSPFASRLPDKKRKDINKILDKYDMSEYDTYELL
                     KRSGARLPIDTLEFVDPIPLDIDKNIIRYFYIAGVRHYAGCEGHECDNSIELESNEAL
                     VLELEPENKFDPHAIKVFDKKENHIGYIPRYYSGELTSIINRGLKYNCIIKFVNKENN
                     CDECIKVKLEILFDEYN"
     gene            18803..19933
                     /locus_tag="KR314_001596"
     CDS             18803..19933
                     /locus_tag="KR314_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH0327985.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
BASE COUNT         6645 a         3499 c         2183 g         7861 t
ORIGIN      
        1 gaaataatac atattaatac tggtgttaaa actaactcta ctacttcttt agaatttata
       61 gccttgtaat aattgagtaa gatatttaat acctttttat acattttaac acctccttag
      121 caatctatgc ttcactcata gcaacttctt gatacattaa attaaaaaaa actgaattat
      181 tctcaattaa atcatctatc aaattattca tcttctcaaa aatgtcttca ctatctatat
      241 gattatctat atcaagagaa acctctattt ctctacacaa ctttataccc tcagtatcca
      301 actttaattt tctcttattt ttctctccct caattattat cctatttatt tttttatttc
      361 taaattcctc tcctttaata tatttttcgc aacatctttt aacttcacta ggtaaatatc
      421 tagttctaaa tgtgggctta tatatcaaat cgttatgtcg tcttgaatta tttaaattag
      481 aaaacaaatt atcttcatca gttccaaact tctctctatc aacatttaaa gttatttttg
      541 aaacaccatc taaatctaat atttgttcta taaaatcttt acttgctatt tgtgtaatat
      601 taattgatgt ttttgacata ttattatcaa atttattatt ttcaatcata tcctcataat
      661 atagatgtaa gtttttactt agttcaatca tgggaacccc cacaaaaact ctttcaaata
      721 atactactgc tatattattt tccttcataa atttaataaa taggtgctgc ttattgctat
      781 ctccttgatc cattttttta ttaccctcta cttctttagt atgagcattt ataatatttg
      841 tattctgatt gtatttagta tattcaagtt tcaccttgat tattaaatca tcctcgctac
      901 attttacttc tgtaatccaa aactttttat ctttagtaat attaattcca tttttgtttt
      961 ctttacaata ttctttatcc ttaaagaagg tctttattac ctcttttaaa atttcttcat
     1021 ctgaatagac actcccatta attttattta taatatctat tctataaaca tcaatatatc
     1081 tttttttaaa attactcatt ataccattat tcctttcaca aatatttata atgatataat
     1141 tctatataat cagtcaatat ccttcaataa tcattcgaca accacaaaat aagtctaaat
     1201 aatactttta ccttattcct gtctataatg tacaaatata tttcattttt aattcattac
     1261 ataaaaaaaa agaccctcta ttaatacagt catttaaatc atttcgatta attctttaat
     1321 ctttttatat acctctttat aattcatacc tttatctatc aacctaggta atttcataga
     1381 tataactctt tcaagtactt gtattaataa aaaaactctt gtatttcatc tgtaaaatct
     1441 tttctgacct tttaccttct gttcttatat ccagccattc ttgatctttt tctgtggcaa
     1501 tatagtaacc atgtattcta atttgtttaa gtgcttttgt aacccacttc gtaaatagtt
     1561 ttgcttctgc tttattactt ctaaatgaca tattgtacac agcttcttct gtaacaaaag
     1621 tagtaccaaa gttaggcaat ttatctttaa agtttctagt gtaggaatct ccgacagtag
     1681 actcattaaa tttcttttta tattctctat ctatatttct taatgtatca cgaatattta
     1741 ctatgcctag ttcctctcct acgtcatttg cattaaacca aacttcttct ccattttttg
     1801 accatatcat ccttacattt ttctcttgta aaattttcaa catactacta cctcctaaat
     1861 tagttattaa taaagaatat tcaatttgaa cacccttatt ttcgaccctt caatatgaga
     1921 gattaaaatt gtataaaaaa agacctagaa atcactctaa gtcatgaatt actcatttat
     1981 cattctcctt atttacaaaa taaaaaagac taagttgggg taacttagcc tttttcaagg
     2041 gggaatatat tatacacttg tttcatacta ccattataac aggggttaaa tatcattaaa
     2101 atatcatctt tttatcatgt atttatcaaa cagttaattt caagccatct ataccaaata
     2161 aatacactcc aagttctttt accatttcat taatccaacg tcttgatgta actactccac
     2221 aatttaatat ctctgctatt tcttcatatg ttttttcatc acaatagtaa agctctaaag
     2281 ctctatactt ttcataagaa tgtaaacgct cttgtcttaa tttcaaagtt ttcattgctg
     2341 aatctatatg tgctatcata ataatagttc ttgctttact tttctttata cttaaaatat
     2401 acaactcttc attatcaagc ttacctaaat catcctctaa gtaatctaca tctttagctt
     2461 cacttaccgc tgagtctata tgtctcttaa aatcattata atgcttcatc aataatctag
     2521 tattatataa aacgcctttt ttcctatctt ctctttcttc ttttcttaac tctcttacaa
     2581 tttcttttat actttcttta tccacttaaa tcactccttc ttgctctctt cgaacaactt
     2641 ctactgcaat actcaacttt agatttatcc aacttataaa atcttctacc acaccaagca
     2701 catcttacta ttttacctgg gcttataagt tccatctttt ctttttactt tcataccttc
     2761 actccctata ttcttttaat tttgctttta ctgcttcaag taatgcactt tgccctttat
     2821 ccttattctc tatagcttcc attacttgtt catctattgt acctttacag attagatgat
     2881 ggataataac agtttccctt tgaccctgtc tataaagcct tgcattagct tgttgataca
     2941 gttctaagct ccaagttaat ccaaaccaaa caattatact tccacctgat tgtaaattaa
     3001 gtccatgtcc tgtacttgct ggatgacaaa gtaataattg tattcttcca ttattccaat
     3061 catatatatc ttttgagttt tctattgttc ttggtttcaa actcttaaac tctttcatta
     3121 aacgattata atcatgttta tagttataaa aaactataat aggcttacca tttgaaacat
     3181 ctataatttc ttttaaagct tctaattttt ctctatggag ttcctttaca tttttatcat
     3241 tatcataaat tgctccatta gctaattgta aaagtttatt tgctgctaca gctgctgatg
     3301 aagctgttat tatatctcta tctaattcta atatcttctc tttttctaat tctttgtaat
     3361 atttaagtat tttactatca aggtgtattt caattttatt atctattttt ctaggcatat
     3421 tcaaataatc ttctgctttt aagcttatac aaatatcttt tattttatta tgtattgcat
     3481 tttctgctcc atttttaggt tgccaattat atatagctcc tgtttgataa tttttttgtc
     3541 ctggctcaaa gtatcgttct ttatatcctg taatagtttt acctagtctt tcacctctat
     3601 caagtaagta catctgtgcc cataagtcta ttaaactatt tggtgttggt gtaccagtaa
     3661 gcccaactac tcttttagtt aaaggcaata ctttctttaa acttttaaat cgttgtgctt
     3721 tatttgattt aaaagaacta agttcatcta tcacaaccat atcaaatggc cattttcttt
     3781 tataaaaatc cactatccag ggtaccattt ctctatttat tatataaata tctgagtctg
     3841 tacttaaagc tctcactcta tctaatttac taccaagaac tttagatact tttagatgct
     3901 ttagatgttc ccattttttt acttcactac tccaggtatc tcttgcaact cttaaaggtg
     3961 ctataactaa aacttttgat atatcaaagt aatcatacat taattcactt atagctgtta
     4021 gagtacagac tgttttacct aatcccatat ctaatagtaa acctatatta ttattatcta
     4081 tagtttttct aattgtgtat tcttggtatg gatgtggttt aaattccatc ccttaacctc
     4141 cttgataaaa tcatctatct cttttaatgt gcttacacac ttaactttaa atcctaattc
     4201 tcttaattct cgcattttat attgttgaag cttccttagt tttttacctg gtgctttaag
     4261 ttctacaaat ataacatgtc cttctggtaa taagacaatc ctatctggca cacctgcctc
     4321 acctggtgaa ataaacttca tagcctttcc acctaacaac tcaatctctt ttttaagtct
     4381 tttttctatt tttgattcta acaaaatatc acttccttaa aatttaagtg tcaacaatgt
     4441 caacagattt tctatatata tgtatatata catattaggc atacatatgt atatacgtat
     4501 atgcctaata ttacttatac tactttatac ttaatttttg ttgacattgt tgacattaat
     4561 acctcaaaca ttgtaattac tgcatcttag gtgtcaacat aagcgtcaac aaagccattt
     4621 tttttgttga cattgttgac acttttaaaa ttttcaaatt tactctgttt ttaattctgt
     4681 tgacactaac ttttacgtac ataagctctc tgcctaccat atattttccc aaaccttaaa
     4741 tttccattat atgatatcca atcatctaat ccttttaata tatcattaat ttctcttgat
     4801 aagataggcg tgagttgttt aggttctcca ttaaatagct caacccatat ctccataaca
     4861 cacgtttttt ctcttaatat tgttccttct tttaaatcac caaaatctga accatgaata
     4921 tattctctct tttctgaaat acttaaatca taccaattct cagtaatagg tttatttaga
     4981 tactcttcga taattccaga tttagcattt tcttctgagt gagttctttg ttgtctttca
     5041 gcttcttttt tctcctcatc tgatagatat agctgttcat tggctttaca taattctact
     5101 gcttctgccc aaatttgatt tctttcatta tcaagttgac cattaaatat actcttgtta
     5161 accttcttta ctcctgtatc tattggccaa aaccttctat tcccggtctt atctcttaaa
     5221 aattctttat cattagttgt tcctataact acacattgac gtaaaaacct tgatgttctc
     5281 ttcccatatg cgaccctata tatatcctct gatttgctta gaaaatgctt aactgcctca
     5341 atatctgctt ttttagtagc catcatttca cccatttcca gcaaccatac tccttgtaat
     5401 tgttcatacg cttctttacc ttgtacagta gttaaactat cagaatacca gtctccacca
     5461 agttttttaa taaaagtact ttttcccatt ccttgaggac cagataaaac catcatatta
     5521 tcaaacttta ttcctggatt aaatactctt gctactgctg ctactaaaac ttttcttatt
     5581 atagtccttg tatagtggtt atcttctgca cctaaatagt caattaaaag tgtatccact
     5641 ctcttaatgc catcccactt taaagaattt aaataatctt ttataggatg aaaagtatta
     5701 ttttcaaaag caattattag agcatcattt acttttgatg gtgatgagat attgtaaatt
     5761 gtttctatat gatgtctaag cccagaatca tcactatcat tccaatcatt taatttatca
     5821 tcttttctcc aaggtaactt acctaaaacg acagctctat ttgaaaattc attataagct
     5881 atttttcctt tcaaatatgg gtcattttct ataaacatta agatattgtt tgttgttttc
     5941 ttataacttc ccttattgtc ataatctaac ctagttaacc attcatcatc ttcaaaatct
     6001 atatcaccaa aatcatcctt agctttatca aggttttctc ttcctatagt ctttcgtact
     6061 ttagtgtcac tgcttgcaaa ttcgctcatt cgagtaaatg aaggtaatct atttacaggc
     6121 gtttcgggtt tagcatcttc atcaagttca ccaaatttat gtatcctaac taaatcaaaa
     6181 gcattgcata aaattccgct tgctgggtct gtaccatgat gactatatga aaacttgtca
     6241 tcataaataa ctactccacc acttgtacta ccttctgaat atgtatacct ggtttcatca
     6301 ataccaggaa tatatacttc atttaagaaa ttttctatag tttcttttat actataggac
     6361 ctgcaaaatg cacctataat tccagccttt tcaatagggt cttgttgttt ttttaattgt
     6421 gtattaaact tttgcctctc cctcgaactt tctggccaat agcttacatc tgtccagtct
     6481 aaatataaat ctagtatttc atttgggttt aaaaactctc catcttgaaa tttaaatata
     6541 tagtctccat caattgaagt acttggaaaa tacataagtc tatgaggttg atatgtagta
     6601 tcatcaaaca tatctattcc tatagtatct gctatcatcc tggaaattgc ttgatattct
     6661 tctggtagta ctggtctagt taaaggtatt acaagtctat atctgggatt attctcagta
     6721 tgtgagtgcg tagaatacat aagacaagca taatcattta ataatgttat atcctcccat
     6781 atatctttat tcgcataatc tatatctaaa gttataatac ttctatttgc aatattctct
     6841 gcttttcgtc ttccattttt taagcttcca cctacaaatc caccaacatc tttaactcta
     6901 tctttttcag tctttgacat cttcctatat tctgtatatg tttcttgagt tcttaatgtt
     6961 ttacttaatc tatttacaag ttcggaccat aaaatacttt tatttttcca atgtgtttct
     7021 aatttatttt ttcctatggc cagcatgagt tggccatcat gtcttacatt tatgtgttca
     7081 atttcactgg cctttatatc cataaatcaa tcacctagtc tttcttataa taatcacatt
     7141 catatccatc tgctttaaga ggaagacctt tagcccaaga tatttctttt cccataatac
     7201 tgttaacttc ttctaaagaa cctccttttt tatctacatc aattacaagc tcatcatgta
     7261 catgcattac aatgctataa cctgcatctg tcacattaaa catagcctct cttaagcaat
     7321 ctctagctgt agcttgaaca atattctcaa ctaacttagg tccataagta tctattcttt
     7381 tccattgttt acttgtctgt tccatacctt catatgttat cttatcccca ctaaatgtag
     7441 tatgaggctc tatcttaggt cttagatatg ataatcttct accacttggt agttctataa
     7501 ataaaactcc tggattataa ataaatttaa tcccatgttg gagttttact atagtcctat
     7561 cttttatggc ttttttagct gccttatcta catcccacca aaatttagtt atatttggat
     7621 tagcatttct ccatgttgtt acaagaggtt gaagctcctc ttcatcaaga cccattttta
     7681 tagcccccat tgatgttaaa gctcctatgc ttcccccata tccaagagct agctctgaaa
     7741 tcttaccttt ttgtctaagt gggtcacctt ttttaatact ttctattgga actttaaaca
     7801 tatgactagc actagcttca tatattttcc cgtgagaatt aaatacatct aatctccact
     7861 tctcaccagc gaaccaagct ataaccctag cttctattgc actaaaatct gatactataa
     7921 atctatgacc ttcacttggt ataaaagctg tccttatcaa ttgacttaag acatctggta
     7981 cactatcata taaaagctct attaaatcaa aatctccttc ttttaatagg tttctagcta
     8041 ggtctaaatc ctctatatga ttttgtggta aattctgtac ttgtactaat cttcctgccc
     8101 atctaccagt cctattagcc ccataaaact gtagtagacc tcttactcta ttgtcattac
     8161 ctttagctaa tttcatagcc tcatatttct ttatagaagt tttggacatt aattttctaa
     8221 gttctaaaat tctaactaca ttttcatcat caacttgttt taatatttct ggaatacttt
     8281 cttttgttag gcttgtaatc tcaaagccaa ctttatcact taaccatttt tttaattgag
     8341 ctggactatt tggattattt agaccagtta ttttaattgc ttcttttgta agtttttcag
     8401 tatatctttt atcacattct attgcattct ctattaactc tgtatcaact ttaataccag
     8461 tatcattaat tctctggtct aaataccata atttaatttc tctttcggta gtcttatact
     8521 tactaagttt gtttcttatt tctctttcaa ctacaacatc ttgtttacaa tattctttaa
     8581 atttattcca tttttccata tcatgtattg gtaaatttct agttcttccc ttgttaactt
     8641 ttgtagcctt acaaggttta cagaaatatt gtattaatgc tttaccttct ttcattttct
     8701 gtttatcttc attaaacttt aaagccttag acacactatc taaacttcct ggaagtccta
     8761 gtgttaacgc ctttatcatt gtacatgacc actcatttgg ttttaaatta atatttaaaa
     8821 atttacttat tgctgttctt tcaaaattag cattaaatgc tgattttata actttattat
     8881 catttaaagc ttctattact tcttttggta actcttcatc atttactaaa tcaataactt
     8941 ttacctcttc attatcaaaa gcataggcaa ataacagtat ctcaaaatta gcagagtcta
     9001 catatctgta gactccaact ttttttatat ctaaatcact atatgtttct atatcaattg
     9061 ataaggtcct cattaactta agaagtcctc ttcttcatct tcatattcaa agtcatctga
     9121 gaaatctgct tctgcactag ctctagctcc tccaagtact tctccatctg ctaacttttg
     9181 aacattttgt aagccacaac ctattccttt atttcctgca ctattatatg ggaaaaagtt
     9241 tatactaact cttccataac aaccactata tacctctgta ttatctaata tttcatttaa
     9301 gtctttatca actattcctg gcttttgtgt actatttgca tttaaaaaat acattcctac
     9361 atattcctct gcttcatcag ctctttctgc atctccatca cgaagaggtg tttttaaatt
     9421 acctggtaat tttccacccc atttagaagt tttaccttgt tctttagctg catcaatagc
     9481 cttctttatt cttcctaaag tcaccttatc tgattttggt attaaaatac aaactgaata
     9541 cttgggctct gcaccttcta ccattgctct gcttttaaag atattgcaat aacttaatct
     9601 tacctttcct gttactacct ttgttgattg tactgaatta ctcataattt tcttcctcct
     9661 aatattttta tatttggtat attgatttta tatttgaagt tatttctcca aatttatcta
     9721 aatctctaac ttgcttacta aacaatttaa ttcttccaca aataactgtt gaagaaacat
     9781 ttaataactc tcctatatca gatatctttt tatcttgtaa aatccaatct actattttat
     9841 caatatcttt taaatatgac tttcttgcaa tttttcttat ataatcaaca tcatagttaa
     9901 attgaatttc ataaaatcca tgtatatctt ttaatggtct tttactatct tcaaatacac
     9961 ttacatctaa accttctata gagtttctat aatgtttttc gccataacgc aaagctttaa
    10021 acatttctat tcttatgtaa gaaacagcca ctgtagaaaa cttgcctctg ttactatcat
    10081 aatttaatgc tgctttatat aacccaatac atccttcttg ataaaactct tcatataagt
    10141 atggatgttg ctctacataa gacttaaaaa atctatttat actaaaatgt acaagattca
    10201 aattattttc aactagttta gttatttctt gattactcat aaaattatat ccccttttaa
    10261 atctttttat agctactagg aattaaatat ttttaattat cctttgctct tactgataca
    10321 aagcatactg gactaacttt tatatgttct ggtatataca ctctatcaag atgattgtca
    10381 tttacttcga ggaaagttga catcacttta tcctttatta aactttctct tttaaaatcc
    10441 atcactataa cttttgcatt ttcttcagtt gagttatatt ctgtaatatt tagtctcttt
    10501 aacatatcta gtaaattatc tttttcataa tctaaatctt tttcgatact cttttttcta
    10561 tcctttaaat ctaaaattat acttatgctt tcatctaatt tatcttttat acctgcatct
    10621 aatggcatgt aatatccccc atttatattt caaaatcttt tttcgctgaa tctatactat
    10681 taatttctga ccttttatca ctttctacta ctaaagtagc ttttccaact ggttttataa
    10741 ttaaatcact tagtagttta gcaaaactct ttttacctat agctttctcc atatcactaa
    10801 ttccttttaa ggtccttggc ttataaattt tttcttcatc ataatctgaa tttaataaaa
    10861 ccttagcaac ttcttgctca tctacatact ttctattact tctgccttct acaagcttat
    10921 atcctggata tttaacacca tgcttttcag cttgttctag tgcataactt tgaacatcct
    10981 ttaaccaatc ttgtatattc ttagcaaaac ctaaaatatc tgctatctca tatttattaa
    11041 gagcaaatgt gtcagcaaaa tcatattttc tagctagttt aaggttatcc tcagctcttt
    11101 ttctgcaatc atttttagct ctacaaaatc cacaatgact gccacttaca aactctcctt
    11161 caccattaaa agccatttga gccttcttct taacattatc tgcccatgta agtaatttag
    11221 taacttctat ttcttcactt gatatgttat caagtctagg ttgaattatt gttgttttaa
    11281 ttaaatcaat atcgtatagc atttcaaact gattatatgc ccctaaacca tatagtctaa
    11341 gttgaggatt ctctatagct gaaacttcta aaccttttcc atactttagg tctataactt
    11401 gaagtatacc atcagatatt acaacaacgt ctcctgttcc aaatccttct ggaacccact
    11461 cactaaaatc gagtctttct tctaacatca ctattacatc atcacatata gctttactat
    11521 catttactaa ttcaactaca ttctcaacat aagattgtat atagtcctcc atttcagagt
    11581 tataatactc acttttctgt atctttttaa ttcttgcatt atatgccttt ttacttattt
    11641 tttcatactc tagcattaat ttaacttctg ctaattcatg tgcaactgtt ccttcttctg
    11701 catatatact agttaatggt ggatagtttt cttctaattt tatactagga gtgcagtgaa
    11761 gccatctatg ggctccactc gcactaagtc ttgcatgttg taatggcatt tatgtacccc
    11821 ctataaactt tctaatttgt tcataaaagc tgaataatct tcttctttta cttcacttaa
    11881 cttactagct ccaaattcac caaataactc cttaagctta tccttcttac ctgccttact
    11941 tacttgtgct gccttagttc ttacctcttc ttttgtgtat ttaacttcac tagttgtatt
    12001 ttcattattc ttttctattt cttcctttgc ttctgctatt tcttctttaa cgtcttcttt
    12061 tacttctacc tttttaatct tttcagcttt tttaacttca actttcttct cttctttcat
    12121 atctgttata tttataggtt ctatctgcat tgcttttcct agattaagac ctcctaaagc
    12181 attcgccact actaatagtg catttgtaaa ttctggtgct tctactttaa cttttacatt
    12241 tacattaact tcaaccattt taaatatctc ctttttgtgt tatactttac ttgtgttata
    12301 ttttattttt tatttttttg tgtgttggtt attttaccaa cactttttta tttaatattc
    12361 caaccgatat tttcttacca gttttaatat ctttaaatac tatatctgct ataacttttc
    12421 catctttttt aagagttact acattcttat tattagtatc aagactcagc aatttcatcc
    12481 ccccactcta ctgcttttaa aagttcatcc aaatttttac cttgatttct ttcaataaaa
    12541 tcatcaactt catatcttga aatttttcta ccatcacctc taactagtga ttttatcaaa
    12601 cctgtgctta ctaacctacg cataaaagct gtatctaact ttaaaattcc ccttgcttct
    12661 tctactgtta ttagataatt tggataacct ctttttatta aaacaactat atctttaggc
    12721 tccagtacct ttaccttttg ttcaatagtt tgactcttaa ctctatctgt ctcttgttta
    12781 cttatctcta tttctattaa actttttaaa ctatcactaa attgttttgc tatactctca
    12841 gaaatatcca tgtgaaaatt ctctcccctc tttttaatta tttatcttcc aactaattcg
    12901 tctaatgtaa tatctaaata atcagctaat tttattagag tatctatagt tggattagaa
    12961 cttttatttc tcagtaattt atataaacca cttacatcta tattcaattc ttttgccaat
    13021 ttataaggtt ttatatctct ttttcttagc atttgcttta tattgtcgcc tattaacatt
    13081 gtattcacct cagttctaca tggtataata taaacatgga ataaattcca aaattttcaa
    13141 aaggatggtg tattatgcag ttttcaaaca atatatctaa agaattacaa gaaagtataa
    13201 gaaagacagt ctctgatact tctaacacag agtattttta ctatgctgaa tttcaatata
    13261 aaattattct taaatctata gaggaatttg aaaaagaact tgatgatgaa catgaaattg
    13321 cactaaaatt gactaacttt ggaaaagatg tcttaatgat tgttgaagaa gtaggttatc
    13381 ataacccttg tctaattcac tactacggca tagtaaatgg agtttattct gaaattcttc
    13441 aacacacatc acagataaac tttatgataa cttcagtaaa gaaaactgac ccttcaaaac
    13501 ctgctagaag aataggtttt atattgtaat tatcattctt attttctgat tctttcttta
    13561 gtaatttcac taatttttct tttttcactt ttattcatct catgtaaata caaaactata
    13621 cattggtaca tataatctat aaaatcacta tttgattgag ctgttatctg actaaataac
    13681 tgtgcttgca attgattttc aagcatggtt attcttttct ctatttcttc tattttctta
    13741 ttttccaatt caatctctcc tttctaaaat attctgtatt tagttttcaa agtactaacc
    13801 taatattgaa gtttgataac tatatccttt ttctatatct ggtaatatgt tatttgtttt
    13861 caataaatca tataaaaaca atcttccctt ttgagtccac ttagttgtca tttttacatc
    13921 aggcattcca ttacttctag ttatgtctat tgtttctgaa tgagtgtatc ccttcccttg
    13981 atgttgttta tataaaagcc attgtccact ttgtttatat tgaatccctc tttcatgaag
    14041 tattttattc atttcttttc cactcattcc atagtctttt gctatttgag ttattgtgac
    14101 aagtccttta ttttttaata tcatatctgt ataatctgcc tttggtttta gttcttttat
    14161 tacttggtct ttcattttac tttctagttg taactgctca tttacttcta cttgctctat
    14221 aagatgttgt aacgcttctt tatatgtagt tggtagttta gtttgcaaat ctatcatatt
    14281 atatgtccca gtactacgta tacttggtaa tatctcatta gttacccaac gtttaaataa
    14341 ttttgcactt ggcaacttag aacttaatat taaagaataa agaccacttt cattaattac
    14401 tttcatattt tgattaccac caggagtcgg tatttcacct actcctttat cttcatcatc
    14461 aacatgtctt ttcaaagcat cagatgtatc tttatatcct aattgttctg caatatcttt
    14521 tccaacaaac caaaattctc catttagttc gattactctt atttctccaa aagtcttatt
    14581 tttgaatatt tgtaaattat tcataatttc tttcctcctt atgattgttt ttgtgaacaa
    14641 taaattttaa tttatgttaa ccttttttct aaaaaaagtt catccattgt tttatttaaa
    14701 atatagctaa tcattatcat ttctttttcc gtaaaatttc tttttccatt ttcttttaga
    14761 ttatatgttg attttgctat tcctatctta tatgctagtt cttcttgggt atatcctgct
    14821 tctattcgac cagcttttaa acttcttttc ttcatgttct tcaccttcca atgttttatt
    14881 tttgtgaact tcatactttt attatattca cacaaacaaa acttgtcaat acataagttt
    14941 atttttttgt taactttttt atttttttct aaacataagt ttacgtttct gtaaacttat
    15001 agtatcattt aattattggg aggtgttgtt tgtggcaact tttggagaac gtttcaaaca
    15061 attacgagaa gagaaaaaac ttactcaaga tgaactagtt tcaaaattca ataaagttta
    15121 ctttacaagt tttaataaat caacaatatc gcaatatgaa aataataaaa gaaaaccaga
    15181 aataaatatt ctagaaaatt gggcagattt ttttgatgta tcaattgatt atcttttggg
    15241 tagaactctt gttagaaatc acatcgatac tgtagccgca cacagagtta atcctcacaa
    15301 agatttacca gaggaagctc aagaacaact caacgattat attgaatttt taatgaataa
    15361 atataaaaaa taaggattga atctatgtta atattaaaaa aattgagaac tgaaaaagga
    15421 atttctttag ataaattaag cacagactta aatataaata aatccacttt atccagaatt
    15481 gaaaatgggt taagagaacc aaaagaatca tttattaaag attgctcaga ctattttgga
    15541 gtatctacag attacttaat tggaaaaatt aacatagatg attcaaataa attaacaaaa
    15601 tgcttaaatt caacttttcc aataaggtta aaagaattaa gaaagaaaaa agaattaact
    15661 caagcagaat tatcaaaatt acttaattgt tcactaagca aaattgctat gttagaaact
    15721 agcaaaagag aacctgttaa agaagacttg ttaagaattt cagaattttt taatgtgtca
    15781 gttgattatc tacttggtaa aacctcaatt gaaaattaca taactactga tgaaatatcc
    15841 aaaataatta aatcttatga aagtttacct aaagaagcgc aagaacatat taatagctac
    15901 atagagtttt tagttgatag atataaaaaa tgaatattta gagcagttca ctcctgctct
    15961 ttatatataa aaaagtaaca catacattct ttttataggg ggatttcaat gaacaaacta
    16021 gacgcacttt tagacttagc aaataatgaa gagatagaaa tttactacac tgacaaaata
    16081 gcagatgaca taaaaggatt gtatataaac agacaaggac taaaaattat atcattactt
    16141 aattcattaa aacaaaacaa tgctaaacta atagaaatct tagcagaaga attaggacat
    16201 cattttacca gtgttggaaa ctatgtatct tcaaaaaaca gttacaaaaa taaaatcttg
    16261 atagacaaaa ctgaaaacaa agcactaaaa tgggcatgtg aatttcttat aacagaagaa
    16321 gaaataatac atgttattaa ttcacacgct acaagtgtat acgaaatagc tgaagaatta
    16381 caagttagca tcaacttctt actaaaaaga ttagaatttc tatcaaaaaa gaaaagcatg
    16441 ttggacttag gaaataatag atttttagta ttaactaatt tgccaaattt ctacatatat
    16501 gaggatattt tttaaactca tttattctac ttttatagat tttttactta ataaatatat
    16561 atttcaatat tattataata aactacacat aaaagctaaa aaattgtaag aatattaaga
    16621 aaataattaa gtaaaaacca gataaacaaa attaagataa tattgtacat aacaaaagta
    16681 tataaagagc agttaatctg ctcttttata taaacaccaa acatacatac attctaaaag
    16741 ggagagatac tattatgaaa ggtggagtaa gaaaaagaag caacaaatgg tattactact
    16801 ttgacctagg catagtatct gaataaagca atatcagctt accttttggt acaaaaaata
    16861 tttcttactt aacaacactc aaaaaaatta ccaaataaat atagaaaact aaaattaagt
    16921 atttataaag taaaaccatt atacacagta gtaattttga tgagtaaaca catattccat
    16981 atgtaataaa gcttgaagtt aaaagtggaa taaataaaat ctagaaaaaa tttttatttt
    17041 aagttaagct tattatggat aataaaattc ttgcaaattt agaatcaaat gatacaataa
    17101 gaatataaaa tacttgtttg agaggattat tttcgtgatt aaaaattttg atgaatggaa
    17161 agagtatgat ggtttttctt atggtagtgg aagaagcgag aaaatctggc taattaatga
    17221 aaaaactaaa gatataggct tgtttaaatt ccctaaaaca aaagaaactg gagaatattg
    17281 ggcagaaaaa ttagcctgtg aaatagctag tttgttaaag attccttgtg ctaatgttga
    17341 gataggtaca tataataaaa gaattggttc aatgagttat ttaatcaata aaaatgatga
    17401 gaaattgatt gaaggcgtgt ggttaataaa caaatattat ccttgttata atacagataa
    17461 gttatatgat tataaaaata atgaatacta ttcattgaaa atgattttta aatctataga
    17521 agaatataac ataaaaaatg attttctaaa aatgttaatt tttgattact tgattggaaa
    17581 tagagatagg catcaaaata attgggctct actagaaagt caaaataaaa ttactcttag
    17641 tcctctatat gataacggat catcattatg ttgttatgtt aatattgaga acatagattc
    17701 tttcttcact gatcatcaaa gatttgaagc tcttataaat acacgttcaa gatcaagaat
    17761 aaggatagat ggaaaaaata aaaaagaacc aacacactta gaagtgctaa aatatttaaa
    17821 ttcaaattat tatgaacaaa ctatagattt tgtagaaaag ataataaatc ttatgaatga
    17881 agtaaacata aatacattaa tagataacta ttcagaaaat attataagcc caaagttaaa
    17941 aaaattgcta aaaatatatc taaaagaaaa agtgttaaga ataaaaaata tatataattt
    18001 gtgaaagagg gataaaatgt caattaaaaa tggaaaagat tttttatatg ttatttggaa
    18061 agatccaaaa agtagaagac aatttataat tggaaagtta gctaaaaatg gattgtatga
    18121 attttcttat gaatatgaaa ttgatgaagc tattaaagca ggttttaatg ctttaatatc
    18181 atttgatgat attagtaaaa catattatag tgaagattta tttagcccat ttgcaagtag
    18241 attacctgat aaaaaaagaa aggacatcaa taaaattttg gataagtatg atatgtctga
    18301 gtatgataca tatgaacttt taaaaagaag tggtgcaaga ttacctatag atacactaga
    18361 atttgtagat ccaattcctt tggacattga taaaaatata attagatatt tttatattgc
    18421 aggagtaaga cattatgctg ggtgtgaagg acatgaatgt gataattcaa tagaattaga
    18481 atcaaatgaa gctctagttc tagagttaga accagaaaat aaatttgatc ctcatgcaat
    18541 caaggtattt gataaaaaag agaatcatat aggttacatt cctagatatt atagtggaga
    18601 attaacaagt ataataaata gaggattaaa gtataattgc attattaaat ttgttaacaa
    18661 agaaaataat tgtgatgaat gtataaaagt taaattagaa atattatttg acgaatataa
    18721 ttaatgtctt cttgtaaata taggagacat tttttatata aacaccaaac aaacatacat
    18781 tctaaaaggg agggatacta ttatgaaagg tggagtaaga aaaagaagca acaaatggta
    18841 ttactacttt gacctaggca tagtagaagg aaaaagaaaa aaagtagaaa gagttggtgg
    18901 caatacaaaa aaagaagcag aaaaagcact aagagaagca ctaaatgaat atgaaaactc
    18961 tggcatagtc tttgaagaaa gtaacatcag cttatcagac tacctagact tttggtacaa
    19021 agaatatgtt ttactcaact gtaaatacaa cactcaagaa agctaccgaa taaacataga
    19081 aaaacatata aagccaaaac taggagctta caaagtaaaa gctttaactc ctgcaatact
    19141 acaaaacttc ataaataaaa agtacaaaga ggattattct caaaatacat tacaagtatt
    19201 aaaagccata ttacataggt cattaaaatc agcagtccat ccttacaaac acatacgaga
    19261 aaaccctatg caatatgtaa gcataccaaa aactaaatct aaaacagaaa ctaataaagt
    19321 taaaactatt acattagaag aatttaatca aatactaaat atatttcctc aagattcatt
    19381 tcaacgtata gttttactaa ttggatttca tactggtatg cgaagaggtg aaattattgc
    19441 actaaaatgg gataatatag accttgataa taaaactatc acagtaaagc atactttgat
    19501 taaaaaacca aatggaatgt ttgaattagg gcaaccaaaa acagaaagct cttgcagaac
    19561 tatatttaca ggtgacactt taataaaggc attaaaagaa cataaattat atcaaaagaa
    19621 aatgaaatta aaatacggag aattttactt tgatagtgat tgggtatgta ccaaagaaaa
    19681 tggtcaacaa gtgaatactc acactttaga cactatagta agacaaattc gagtagcttt
    19741 aaacaatgac ttccattttc attctttaag acatgcacat gctactctat tattagaaaa
    19801 tggtgctaac attaaagaca tacaaaaccg tttgggtcat agccaattat caactacaat
    19861 ggatacctat tcacatgtaa ctgataaaat gaaaaatgaa actgtagata tatttgaaaa
    19921 aattacaaat tagagtttgc cacccaaaaa tataatacgg tggcaaatgg gtggcaaaat
    19981 ctaatttatc tattttaaaa gctaaaatta tcaaatttat atagtcagct atacgcttgt
    20041 aatttcaagg ctttagagta tataacaacc ataactaata taaggtatta ataataaatc
    20101 taacgaataa aacttaatat ttacttaagt tgtaatacta ttctaaattg tataattaaa
    20161 atttaataag ttcttaatta tatattca
//
LOCUS       DAEPLF010000050        20010 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEPLF010000050 DAEPLF010000000
VERSION     DAEPLF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20010
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.050"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..407)
                     /locus_tag="KR314_001597"
     CDS             complement(<1..407)
                     /locus_tag="KR314_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327986.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDNRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIEVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDV"
     gene            complement(550..807)
                     /locus_tag="KR314_001598"
     CDS             complement(550..807)
                     /locus_tag="KR314_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327987.1"
                     /translation="MILNRTELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FSNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            1133..1645
                     /locus_tag="KR314_001599"
     CDS             1133..1645
                     /locus_tag="KR314_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0327988.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEETYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDISNYYLVANYI
                     RCLKKYKLID"
     gene            1900..2604
                     /locus_tag="KR314_001600"
     CDS             1900..2604
                     /locus_tag="KR314_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBH0327989.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTDGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            2601..3371
                     /locus_tag="KR314_001601"
     CDS             2601..3371
                     /locus_tag="KR314_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBH0327990.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            3368..4144
                     /locus_tag="KR314_001602"
     CDS             3368..4144
                     /locus_tag="KR314_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBH0327991.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            4199..4891
                     /locus_tag="KR314_001603"
     CDS             4199..4891
                     /locus_tag="KR314_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0327992.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            4910..6334
                     /locus_tag="KR314_001604"
     CDS             4910..6334
                     /locus_tag="KR314_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0327993.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMPYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANSRGR
                     FVFAVIIMALFSPFIYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMKGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(6574..9042)
                     /locus_tag="KR314_001605"
     CDS             complement(6574..9042)
                     /locus_tag="KR314_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0327994.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASRVSPKIAMAGSTTKIKRKIKREKAIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIDKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYNSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNISLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(9045..9713)
                     /locus_tag="KR314_001606"
     CDS             complement(9045..9713)
                     /locus_tag="KR314_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0327995.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLKINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            complement(9999..10526)
                     /locus_tag="KR314_001607"
     CDS             complement(9999..10526)
                     /locus_tag="KR314_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBH0327996.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            complement(10743..11903)
                     /locus_tag="KR314_001608"
     CDS             complement(10743..11903)
                     /locus_tag="KR314_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0327997.1"
                     /translation="MDKISNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNINMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNPHFGMGLHISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            complement(11891..12565)
                     /locus_tag="KR314_001609"
     CDS             complement(11891..12565)
                     /locus_tag="KR314_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0327998.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDSLKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            complement(13386..14213)
                     /locus_tag="KR314_001610"
     CDS             complement(13386..14213)
                     /locus_tag="KR314_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBH0327999.1"
                     /translation="MNFYKIAVCQMMTTENKIENINHAVDMVTEAAINGAKIIVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEYGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGVEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            14595..14915
                     /gene="sugE"
                     /locus_tag="KR314_001611"
     CDS             14595..14915
                     /gene="sugE"
                     /locus_tag="KR314_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBH0328000.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            complement(15048..15728)
                     /locus_tag="KR314_001612"
     CDS             complement(15048..15728)
                     /locus_tag="KR314_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBH0328001.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            16124..17176
                     /locus_tag="KR314_001613"
     CDS             16124..17176
                     /locus_tag="KR314_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH0328002.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGTIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPMTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            17223..17639
                     /locus_tag="KR314_001614"
     CDS             17223..17639
                     /locus_tag="KR314_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0328003.1"
                     /translation="MIHMTGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRRLEDRIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            17668..18141
                     /locus_tag="KR314_001615"
     CDS             17668..18141
                     /locus_tag="KR314_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0328004.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDEHLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            18188..18946
                     /locus_tag="KR314_001616"
     CDS             18188..18946
                     /locus_tag="KR314_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0328005.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNENVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            18970..19809
                     /locus_tag="KR314_001617"
     CDS             18970..19809
                     /locus_tag="KR314_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0328006.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
BASE COUNT         7021 a         2587 c         3321 g         7081 t
ORIGIN      
        1 acatctccca ttaacattac tacctctagg tttttaaata atttaatttc ttcttctaaa
       61 ataggcatat gtaatttaat tgtatcacga gttattgtat attctgattt aggaagtttt
      121 actgcattag ttatatagat acccatatct aggatatcat ttatgttaga tacttctatt
      181 cctgcactgt caaataaagg aatagtagtt tttaaataat cagcatcttt atctacacta
      241 taaaaatcgt cattggtgtt agttgggaca acctcattaa tcattatagt ttttattttg
      301 tctgtttcta ttgttatatt aggaaggtag gatattattc tgttgtcatc aaaaagattg
      361 ttctgtttaa taaattcact taactttgat tttatattta ttttcatata acgagtctcc
      421 ttagttttaa gctttaaatt actataaaaa gctagtttta aagcacacaa ttttgaaaat
      481 atagtagcat atttgaaagt ataataacat ataaaacatg acaacagaat gtcatgtttt
      541 ataaataatc tatcgcaact tagaatttat tagagactct gcctgttcat aatcgtcttt
      601 atgaacataa attaagtact cataagagaa atcttccttt tgacctaaat tgccaagtgt
      661 tatttcatta gaaaaaggtt ttttagagta agcattattt ttcactttat acttatattt
      721 aattttattt actgataata tattgcatat ctcgttaaat ctatttaaag aatatcctcg
      781 gaaaagctca gttctattta aaatcataag gaaaacctcc ttttaaaagt aatatattga
      841 aaagcatagg agaaatgtac tataagttgt tataaataaa aggtagcaaa tttaaattga
      901 tttgtaaagt ttttaagagt attatattag tgataaaata aataattttt ataatctaac
      961 taataacaat ttaaaatagg taacttatgt aagaatcgta gggactacgg agatatcctg
     1021 tagagaatta ataagacata tttaagtatg caaaattcta ttaagtagga accttgtaac
     1081 tttagtcatg tgagatttat aaggatattt aagtattgaa aggagagttt aattgaaaaa
     1141 aatatctcaa gaaaaaataa gtacacatat ccaagaagta ccacaggaaa aaattgaaca
     1201 gtggaaatct ttatatgaaa aatataaaag tattttaaaa gtaaattcta agtctgtttt
     1261 agaaataata gagtttttaa agcgaaaata tgtaatagta gaagaaactt cagaggaatt
     1321 taaacatgta gttatcaata atattaagtt aaacaagcct ttttcacaaa agatttccag
     1381 tgataaagaa ttaaaaccta ttgtatttac aattccaaat gaaggaaatg caaaaaaatt
     1441 gtacgatact caagaggaaa cttatagtgg ctgtaagatt attgtaggta tggaatttga
     1501 aacaggttgt gtttttgtag aaggtagtga tgagcttaat gatgaaataa cttcttttaa
     1561 aggattagat aaatatgata taagtaatta ttatttagta gcaaactata ttagatgtct
     1621 aaagaaatat aaattaatag attagtgttt gattaataaa ttagtgcttg atttaaggat
     1681 tatagagtaa atttgaagtt agtgtgataa tgaaactaag cttaaatcta atataactgt
     1741 aaaacataaa cttaaataga gttgccaaaa taaaattggt aactctattt tttattttaa
     1801 tttttattaa aattagtagg tgttatagaa ttgttatttt ttcatgttag tattctctta
     1861 gtaatagatt taaggtatta caagaaggga gaaattaata tgaaagattt aattcttgaa
     1921 acaaaaaact tatcaaaaag atatggagaa caaatggcag ttaacaatgt ttctctacaa
     1981 attgaacgaa atagtatata tggactttta ggaccaaatg gagcaggaaa gtccacaaca
     2041 cttaaaatgc ttgttggtct tcttcgccca acagatggtc gtattatttt tgatagaaag
     2101 ccttggaaac gagaatctct tgcaaaaata ggttctttaa tagaatcccc tgcattgtat
     2161 ggaaacctta cagcagagga aaatttgtta gtacatacaa aaatattggg aatacctaaa
     2221 gataaaattt atgaagtgtt ggaaatagta aatttgaaaa atacaggtaa aaagagagct
     2281 tcacaatttt ctatgggtat gaaacaaagg ttaggaatag ctattgcact tttgagtgac
     2341 ccagagttgt taatactaga tgaaccaacc aatggtcttg acccctttgg cattcaagaa
     2401 ttgcgtgaat tgattgcttc ttttccaaaa aaaggtataa ctgtaattct ttctagccat
     2461 attctatcag aggttgctca agttgtagat aatataggaa ttataaatgg aggacgacta
     2521 ctatttcaag gaatgcctga ccctaatgaa aatctagaag aattttttac agatgttatt
     2581 cttaaaggag gacaaaacag atgaatgctt tacaatcaga acttttaaag tataaaagaa
     2641 catttatggg taaacttatt gtttttttcc cagtgtcttt tgcagcatat gcatttataa
     2701 tgcaatcaac actcatgcaa aatccattat ctcaaacaac ttcttgggcg tggcaaagtc
     2761 ttttagcatt aatttttaac tggtggtcat ttttatttct gcctattgga tttgcattgt
     2821 ttgctacttt ggtagctttc caggaaaaaa aagctggaaa ttatcgtgct ttacgtacac
     2881 acaatgtgtc tcctatgaca ctttggatta ataaagttat agctatggca gtctacagtt
     2941 ttatttcaac gttagtttta atagtcgtaa caattataac tggtttaata ttgaaagctg
     3001 gtccagttcc ttttggacag attatagggg caagtattgt atgctgggtt gtttctcttg
     3061 ctatacttcc acttcagcta tggctagcta catggaaagg tatgttccta agtatgggag
     3121 ttggagcttt aggtatgata tttggtgtcc ttgctgcgac aaaaccattt tggattgcag
     3181 taccttggag ttgggcagtt agaatggtat gcccaatcat caacatacat cctaacggta
     3241 ctatattaga agctggagac ccacttttga atacttcagt gattcctatt gggattgttg
     3301 tttctttggt tgtgtttata gtattaacag cgcttactgc agcttggttc aataggaggg
     3361 atgacaaatg ataaagctat tatcatcaga atggcttcga gcaaagagga cagctgtcca
     3421 gtggcttact ctttgtatgc ctattatttt ttcattatgt gtggtttttt atctcacaac
     3481 taaatctgga agtacacaag aatttgcttt tgaaggattt tttacagtat ggactgtatt
     3541 catcattcct attggagtag gtattttagc aggatttata gtgcaggagg aagagcttgc
     3601 tggaaatttt aatggttttt tatgtgtagg tatttctcgt gttagattat atctgggaaa
     3661 atttcttttt cttctgtttt gcctaactat atgcacattt attgctacgt tgatactttg
     3721 cataggaatg agtattgcag tgccatctgg agcaacaata tggttattcc tgtcagcagc
     3781 aggacttgtg gtgattggga cacttccact attagctatt catctttggg tgagttttgc
     3841 atggggtatg ggtgcatcta ttggtattag tatcggtgga attttgatgg cagcaatact
     3901 tggattaact agcgttggtg aaaaagtatg ggcatttgtt ccatggacat ggccagtaaa
     3961 actaggtatg cttccaggaa tttattttat taaagaagct ggaactattt caactgaggc
     4021 attttactct aaagtgatgc aaacagctag tatagagctt atagtagtag caataggttt
     4081 gattatattc ttgataggag gcgttatttg gtttaaaatg tgggagggaa ggaaaagtta
     4141 ttagtagacg aatattgttt taatactgac cttcccataa gagaaaggtg gtgttatatt
     4201 gagtaaaata ttgattattg atgatgaaat ggacctagta atgctacttg aggatgaatt
     4261 gaaggcaaaa ggacatgaag tgttgatagc ttatgatggt caggcaggta tagagttatc
     4321 aaaacaggag cctgacctta ttatactgga tattatgatg cctaaaatga atggatttga
     4381 ggtatgtcag gcaattagag atgatgtttt atgcccaatt atatttttaa gtgcaaagca
     4441 atcagaaacc gacaaaatca aagggttgac ctttggaggg gatgattatg taacaaagcc
     4501 atttggactg cgtgaactta tggcaaggat tgaagctaat ctgcgtaggg aaaagcgttc
     4561 acagtatatc aatgaagaga acaagagatc tactttgtat tttgggaagt tatgcttata
     4621 tatgaaggaa cgtgcaatta aaattgatgg taagaatatt gatttaacaa aaatagagta
     4681 tgatattgtt gaattactag cactccatgc aggacaagtt ttctcaagag aacaaatcta
     4741 tgaaaaagta tgggggtgtg attctgaagg tgattcctca actgttgttg aacgagtaaa
     4801 aaaaatacga gctaagtttt ctgctgtgac accagaaaaa gaatatattt ccacagtttg
     4861 gggtattggg tataagtgga ataaaatgta aagagagaga ggttattaaa tgaaaaatca
     4921 atcccttatt acccaattta gacatacttt tatttttatt ataatagcca gtatagttgc
     4981 cacagtgatt acttatgtat ttgctctcta tttgtatatt cattccttaa ataaggatat
     5041 ctatccacca aactattatg agcgacaagt tcctagaatt gaaaaataca tcaatgaaaa
     5101 aaacataact ttgttatcac aatcgaatga agaaggttta aaaagaacga ttcgtgggga
     5161 tgatatgcca tatcaagttg tggataataa tggcaatatt ctatatggta ctaaccccaa
     5221 aaaactattt aaaacaaaag aagaattatt taataatttt atcaataaaa ctgttcgtaa
     5281 aggtggttat atccatactg ttccaatcaa aggggataat ggaaagattg agggagctgt
     5341 tattctattc tatcaagtca aaataacctt tgcaaatagt agagggagat ttgtatttgc
     5401 agttattata atggctctgt tttctccatt tatatatatc gtgggtttta ctagatggct
     5461 ttctaagcgc tttgtaaaga atatcaatca accattacat ctattgattg atgcgtccaa
     5521 gaagataaaa gaaaaggacc ttgattttga aatcgactat tattcagata atgaactcgg
     5581 taaactttgt agtgcctttt ctgaaatgaa agacgaatta aaaggttctt tatctgcgca
     5641 gtggaagatg gagcaggaac gtgttgaaat ggtggaagct ctagcacatg atttaaaatc
     5701 tcctctttct attattttgg gatatacaga tgcacttatt ggtaataata cagatgacaa
     5761 tgaaaaactt cataggtatc ttacagtgat aagagaaaat acagaaaaaa gtgctgctct
     5821 tgtccaaaag atgcaatata cgtctgactt agaaaagtca aatatacaat taaatcttgt
     5881 tcctataaat ttacctgaat ttttgaggca aaaggtgcaa gactatgaat tacaggctca
     5941 tcaaaaggaa gttgaactta tattgaaaat gaaggggaat atacaatccc caattcaaat
     6001 cgacgtggat aggttgacac gaatttttga taatatcata tccaacagtc tgcaatacac
     6061 tccatcaggt ggtaatatta gtattactgt aaaggatgaa aaaaattgta tttcttatga
     6121 gatttgtgat tctggtagag ggtttagctc taaagattta aaaaaggctc ttgataagtt
     6181 ttatcgagga gatgaagcta ggcagacaaa aggtggacat tctggtcttg ggctttacat
     6241 tgtcaaacaa ttagtggaac agcttggagg ttctgttaaa atagaaaatt caaaatcagg
     6301 tggagcatgt gtgaaatttt ggcatagcat ttaactatat attttaaatt aaaggggagt
     6361 gtctcaaagc gaaattttta attcgtgagg cactcttttt ttgtgaagtt aaatatattt
     6421 tcattattcc gacaatgaaa aaaggcttat ctctattttg agacaggcct tttttaaagc
     6481 ttatattata gttaaaaaga tttattagtt agatttgaaa gaaaatagag acagtcattg
     6541 ataatttaat ttatattatt atctgtaaaa tagttataaa ttgatatcta tacagtcaat
     6601 tatattcatc tttttaactt ttcttaacgg aataagagta gctataatac atgcaagtac
     6661 agaaataata gtagcttgta aaattgaagt aattggtata ttagtgaaat caagcttttc
     6721 tatagttgcc atgtttataa taatagcact ggcataccca gcaattgaac caaatatagc
     6781 agcaaaaatt ccataataaa caccttccca taaaaacatt ttatataagc taatattact
     6841 cataccaata gctcttttaa ctccaatctc attagtccta gtatttatat ttgtatgagt
     6901 tgtgttaatg atgtttaaag taccaatcaa tcccacaaat aatataactg cccatgctag
     6961 aaattctatt tgtttaaaac tctctttaag ttgcttatct gattcaatat atgatatcca
     7021 atgagagcca ggattatttt cacaaatctg ttcaatagac aatcttattt gttctaattt
     7081 tgatttatct ttagcataaa tatttatctg gttgaacttg tttttatttg tcaaggtgtt
     7141 ataaacagaa ttatatacaa taacatcaac accatttatc cagccattac cttgtaaaaa
     7201 gaacatttta tctactgtac tatatatatc tagttgtttt ttgttgattg tgatactatc
     7261 tttaggtttt aaatttgtat gtctagtttt tacacctggt gtagaaatag caatcggatt
     7321 ttttataaaa cagatagaac catttttaaa gtcttctagt atagaatctg taatagaagg
     7381 cattaagtct tttaaagtct gctcgtcaat tccaatgatt tttaacatct caccactatt
     7441 ttcaaaattt atatctgtat ctatttcacc ctgtttgtat tcagagtact ttataaagga
     7501 agtgcgattg acgtttttca ttttatctat tttatctagt gttgatttat caaaaccaga
     7561 tatttcattt gttaaggaaa aatctccttc ttttaaattg cttactgacc tagatacatc
     7621 taatacatta gagaactcac tcaatgcaac gaatactgtt atactcataa ataatgatag
     7681 tatagttatt atagttcttc ctttatttct ttttaagttt agccatgcta gatgtctttc
     7741 aaagtttttt attgcttttt ctcttttaat ttttcttttg attttggtag tactaccagc
     7801 catagcaatt ttgggagaga cacgtgaagc atatattgct gatggtaatg ctgaaataaa
     7861 tgaaaaaatc aaagatacat tagtgcttaa tactaatggg aacatatatg ctgttgtatt
     7921 tttatgtata agctcagtaa tttctttatt atcatttgct agaagtatat ctggatttaa
     7981 gatattagtg accattccag ttatgccttt agaagagata attccaatta cagctccaat
     8041 aggtatggca acagtacata aaattaagat ttgtaaaata actatcttat atatctggtt
     8101 tggttcagct cctattgctc ttaaacatcc atactcttta attttctttg taacagagat
     8161 ttttaaaata ttatagatta caagtccaga agccagtaaa accaataagg caacaaataa
     8221 agataccaat ataatcatag atataccatc actattagaa ctattttcat ctttttcaaa
     8281 ctgtatctta agagcattta agtaaatcca attatattga attgaattgt gagaaagatt
     8341 tattttttta gctaattgat ttactatttc ttgaaacttt tcttgctgtt ttattttaaa
     8401 atccaaagag gataaaatat atctcttagg taatagattt tctgatgtac ctttacctac
     8461 aataccattt actataccac ttacatatcc tgtataatca tcctctaaaa tgccactcaa
     8521 tttaaagtta gctgtgtaat tatatggtgg tatggtatca tttaaaagtg aaatatctaa
     8581 attcataggt atagtcactc ctagtcttgg ttttataccc ataagcttta gagtgttttc
     8641 atctaaggct atttcgtttt tatctttagg caaatgtcca gattttaatt tcattaattt
     8701 tgggtagtta gaaagtcctg ttttatcata ctcttttaca agtacagaga taccagaatc
     8761 ctttatttta ctactgccaa tagttattga agtaccagct tgataaactc tatcatcttt
     8821 ctttatgtca tcaacttgat ttttagtaag ttggtgaaag gatacatgtc tatcaccatt
     8881 aaaagaacgg gcttgttcaa ttcttaaatt ttgtaggata ccaattgact gtccaaccac
     8941 agttgtcata atagttgata gtactattgc tataatgatg aatgtagtca taagtttctg
     9001 gctttttaat tcttttattg aaagtgaaag gtaattcttc atttctattt cacctccata
     9061 agaacaccat caataattga aatttttcta tcagcaagtt ttgcaattct atcatcatga
     9121 gtaattacca ctaaagtctt tccaaactgt tttgaagttt ttataagaag attcataact
     9181 tcactgccac ttttactgtc caagtttcct gtaggctcat cagcaaatat tatatctggt
     9241 tttgcaataa gtgctctagc gattgcaact ctttgttgct gaccgcctga aagttcatgg
     9301 ggaagatgat tgagtctatc tgcaattcca agtaatgatg aaatttcatt taaatagtct
     9361 ttatcaggtt gttttttatc tagaagtaca ggcattgaaa tattttcaga tgctgtaaga
     9421 acaggtaaaa gattaaattg ttgaaagacg aatccaaagg attttcttct aagtgcagat
     9481 agttcttttt cagtgtgttt atatatatct ttatcatcaa gtaacacttg tccagaggta
     9541 ggattatcta atccacttaa aatatgtaaa agtgtgcttt ttccacttcc agaaggtcca
     9601 attacagaaa caaattcacc tgagtttatt tttaaattta cattattaag tgctataact
     9661 ttattgttat tagaaccata tatttttgat aattgttttg cttttataat cataaaaaac
     9721 actctccttt tctatataag aagagtgtaa gttttaaaac tcaatttttt cccaactttt
     9781 agcttaaatc taaaaagtct tcactattta cggataattc gcagtgctga taagcagttt
     9841 cagtatcatt tttaatttaa aatgtaattt atgcactaaa ggctttacaa tacaactcta
     9901 ttgctttaaa actattttac acataaactt gtggataatt ttttatgttt tacttcctca
     9961 ttattatata aattattttt gtcacaaatt ctaagttgtc acataaatat actttttata
    10021 tcacgattta attcttttga ttttatcata attgctattt cttcaaaaga agaaagaaaa
    10081 gccacgatac atatcataat tcctgcaaca tcaagaccaa agcaaatata gagtaatgga
    10141 aaagcaaaga tagaaattcc agtgatttta ttcatcaaag tatgaagcga tgaaaatgtg
    10201 cgatacttaa aaaaaccaat actgtaacta atcaaccgta ataatgctat tgcaacaata
    10261 caccaccata gatacatggg aagagcaata ttttttccga cagtaaccaa aattgtaacc
    10321 gcaaaaatga aatcagcaat actatctaac tttgcaccta ttccactttg ttgtctcaac
    10381 tttcgggcaa taagaccatc aagtatatca ctaattccac accaaaaata acatatccag
    10441 aaaggcagtg aacagaatgt agaactaagc attagcaatg caaacacaat acgagagaat
    10501 gtaatattat tagcaaaact tttaatataa tcacctccaa aattctagtt tcatactatt
    10561 ctacatattt aaatatatat ttctattatt gtttcaaatg ccttttgcca aaatattata
    10621 attgaagcaa ctcttacaac ttctacaaaa tagtatatat attggttgta aattgaaata
    10681 cattttataa attctaaact aacaaagctt atataagaac aatgaaagct tacaaaatca
    10741 ttttaatttc ttgttgagaa tttagcagta attttagcac tgttagtaga attattttca
    10801 atagaaatag agccaccatg tttttcacat aatatttttg agatatgaag acccatacca
    10861 aagtgtggat tgctgtcatt taaagtctta tctctataga aaggtcttaa agctaatttt
    10921 agagattcct tactaaatcc tataccatca tcttcaatta caaaggtaag gagttcttgt
    10981 tcaagatata taagtatgga tacattattt ctagcatatc taaaagcatt cgatattata
    11041 ttttcgacga ctctaagaat gatattttca tcaaaaaata gatttatatt atttatttga
    11101 cttttaagtg taaaagattt tccttcttgt ttagcaatga tagatatatt ttcatctaag
    11161 ttttctataa acttgtttat attcgacata gattttgaaa caactaaatc ttctaatcgt
    11221 tgagcattac tcatattttc tacatagtat tcaattctgt taatatgctc agacataagt
    11281 tgtgtggttg ataaaatctt ctcatcacta agtttgcctg ttggtatgta ttttgttaaa
    11341 tagtttgagt aaccttttag aactgttagg ggatttctta aatcatgaga aaaagcagca
    11401 ttaagttgtt ttctatcatt aatcatagtc cacatattta tgttgttttt atttagttca
    11461 gacctcattt tttcaaaagc attacataga ttacctaatt catcattatt attatattct
    11521 aagcaaaaat ctaaattgtt gttggatatt tccattgcac ctttattgag gatttctatg
    11581 ggtgttttta attttacctt gtaaaaaatt atatcagcta ataataaaag tcctataaaa
    11641 aatataagtg gaagtacaag ctgaagtatg ttgattatat taagtaatgt tttatcataa
    11701 tcactaaatt ggttcacatt gttttgaact atggttataa tacctgtatt tataggtgtt
    11761 tttaaatact tatcttgtat agaattatat agattattaa gtaaaatgta tgcgaaagta
    11821 gtaagtactg atgctataat tagtgaagat aaagtaagaa caaagaaagc tttttttaat
    11881 gacatattac ttattttatc catttataac caactcccca gactgtttct atataatttt
    11941 caagagtatg ttttgatagt ttgattcgta tttttctaat gtgttccatt ataacatcac
    12001 tatttccatt gctattgtat ccccagactc tatcatatat tttttcttta tcaaatacct
    12061 gtccaggatt catagagaga agctctacta tatcaaattc cttttttgaa agtgaaatct
    12121 gattattata tatacaaatt aacctttttt cgtaatctat tgttaaatca ttataaaatt
    12181 taattttagt ttgatttgac ttcctttgtt cacgtcttaa atgtgcagat actctagctc
    12241 caagttcgtc taaatcaaaa ggttttacaa tataatcatc agcaccagca cgaaaaccat
    12301 ttatcttgtc agtattttca attctggctg taagaaatag aatagggcat gatacataat
    12361 ctctaattct attacaaact tcaattccat ctatttcagg catgtttata tcaagtagaa
    12421 tgatatctgg atttttggat atttttttta atgagtcttt tccactttga gcagtgtaaa
    12481 cctcataacc attcatttca aagtaatctt ttaagagtgt tattatacct tcctcatcat
    12541 ctacgacgag tattttattg ttcataagaa tgctcccttc taaattaagt ttgtatagta
    12601 tttatatttt agtataaaaa tctcaataaa ttctcaaatt cttagttata tttcaaaact
    12661 taatcattaa agttagtata tttttatata ttaaataaat caaggatgaa attattagaa
    12721 attttaatag aatatatatt tgatatttaa tatttattgg aatgcaaaaa cacagctgat
    12781 gaaatcttat aatttaagtt gaaaatggat aaatagaaag gaaggatttt aattaaaaag
    12841 gtagattaat taaaaagtat ataaattata aaaatgaatt aatctacatt aattagttga
    12901 tatatcaatt attattattt tttcagttgc ttaaaatata cttgatatat caactaatgt
    12961 tgttttttaa ttaaaagaaa ttttaggaaa gataatatta actaggagag ataatatcaa
    13021 cattgaagaa ggtagagcaa tatttttaca taattgtgaa cattatctgg catgtgtgca
    13081 ttggtgtttt aagcaagcaa ttcaatagaa agatgtgact aaaaatagaa agaaatacca
    13141 taatccacat gtaaagctag aagacataat aagtaaaaaa agtaaagata tttaataaaa
    13201 gaaataacta atagtgatta aattagtaaa tgctgacaat tagcataatc attagtaatt
    13261 attagtatta tttaattaaa ttaattttag cattattttt attaaattaa ttttaaatca
    13321 aattaatgag aaccccataa atttttttaa cacttaattt atgatttatg gggttatgtt
    13381 ctgttttatt tcaaagtatt aagttcatat aaatcagttc ttctgtgctt aagaagtggt
    13441 aactgttgtc ttatgtcagg aattaaatct ctatcaatgt cagcataaat aatacattct
    13501 ttttcatcag cttgagcaat tattcttccc caagggtctg aaattcttga atttccaaaa
    13561 gctatataag aagcattcat atttctagca ggtgcagcac caacatagaa tatctgatta
    13621 tctaaagcac gcattcttat agataactcc caatgggcgg gaccagtagt catattaaat
    13681 gcagcaggta agataacgat ttcaacacct ttaagagcca ttaaacgtga taactcaggg
    13741 aaacgaatat catagcaaat agctatacca acttttccat attcggtatc tattacagtg
    13801 acatcatttc cagctgtaag agtatctgat tctttaaaac taacttttcc tgtaacttca
    13861 atgtcaaata agtgcatttt acgatgtttt ccaattagag ctccattttt atcaaataca
    13921 tagcaagtat tatagatttt tccatattct aactcaggaa tactaccaga aactagataa
    13981 attccatgtt tctcagctaa tttactcaat atcgttgttg tttcacctgg atattcttca
    14041 gcaaatttag gaaaatattt attttcatat gggcagttaa acatttctgg caaaactata
    14101 attttagctc cattgatggc agcttctgtt accatatcta cagcatgatt gatattttca
    14161 attttatttt ctgtggtcat catctgacat actgcaattt tatagaaatt catagaaatt
    14221 actcctttca atatttttat gttattataa catattaaat aaaaattaga tattcttaga
    14281 ttgacctaaa tgggtatgga gagaagtgaa taagaatatt aaaaggtata taaaagttat
    14341 ttttattata gatggaaccc atatattgac tttttttata tatgttgata gtattattat
    14401 agataaaata gaataattaa tagagtttta tagggttttg caattattaa tgattggcct
    14461 ggtccgagat aaaacccaca acatagtttt tgttgtgaac acggaaggac aaaaacctgg
    14521 gagatatttt ataatatttc tcaggttatt ttattggaaa aataagagat acatttgaaa
    14581 agagggatga gtttatgaaa tggttatttt tgtttgttgc aggtttgttt gaagtgtttt
    14641 gggcttttca actaaaaaat tcacatggat tttctaaatt aactccaagt atacttactg
    14701 ttgttggaat gattgtaagt ttttattttt tgtcattagc actaaaacat cttccacttg
    14761 gaacttcata tgcaatatgg actggaattg gtaccgtcgg tacagctatt gtaggcatgg
    14821 ctatcttaaa tgagcctatt agtatatcaa gaattgcatg tataggcttt attgtggtgg
    14881 gaattgtagg gttaaagcta ttatctacta attagagtat attaatttaa ataagctatg
    14941 aaattaaagg cgagaaaatg tttaaaaata atcaacattt aaaaagtctt tacttcatag
    15001 cttatcttat ttataagttt attatatttg aaaattatat gtatatacta tttatctaat
    15061 aaaagagcag ttaaatccat agggtcaact attacaccat ctttataaac acgcatattt
    15121 ccagatgcta tttcatctat taagattact tcatcattgt agaatccaaa ttcaaatttg
    15181 atatcataaa gttcaagacc tttttcagct atagtatcac gaactatatt agaaattttt
    15241 tgagtcatgc ttttcattga atcaaattga gcttgagtca taatgtttaa agcttctaaa
    15301 ccttcagatg ttataagagg gtcacaacgg tcatcatctt ttaaagttgc ttctacatag
    15361 caatctagtt tagcaccttc ttcaacacat gaaccataac gacgtaagaa actaccaaca
    15421 gctctataac gacaaataac ttctaatcca ttaccaaaaa cttttgctgg tagaacttcc
    15481 atagttgctt cttctaaatt agcacttaca taatgagttt taattccagc attgtttaga
    15541 atttcaaaga acttaacaga tgtctctaaa ttagctctac caattccatc tatacttaga
    15601 cctacactat tttcgcctgg gtcaaatact ccatctttac cagttacatc gtctttaaat
    15661 ttaagtaagt aatttccatt atctaattca aaaacatctt tagtctttcc ttgatacact
    15721 tttttcataa acaacctcca aaaagttaaa ttattatatt gaataaggca ttagtgtcaa
    15781 aaaagttctg atattcgcat gaaaatttat attgatacct tatatttaat attatatcac
    15841 aaatagaagg gaaatttccg aacaatttta taaaaataac aaaaaaagta cattttataa
    15901 aacttataat aagagaacta atataaaatg ttagtgtcgt ttatattgac acacataagt
    15961 tttaatataa gaaacttttg acacactttg acacacattt aatgataatt ttataaaatg
    16021 catgaatgca atgcttacat atattggcat gaaagttgct ttagatattt aacatataaa
    16081 caatagggat atattctcaa ataaaaagta ggggtggtta atagtgaagt cagttagatt
    16141 ctatggtata agagacacaa gagtggagga tgtggatgta ccaaaaatat tagaaaaaga
    16201 tgatgtaata attaaagtaa aagtagctgg aatatgtggt tctgatatat caaaatattc
    16261 aaagacaggt ccacatatgg ttggagaaat tttagggcat gaattttctg gagaagtggc
    16321 acaggttggt aaagaagtaa ggagttttaa aataggagat agagttgctg tttgtccagc
    16381 tatgccatgt tttgaatgtg atgaatgtaa aaaaggattg tattcaagat gtaataatgt
    16441 agcaataata ggaaataaag aacttggagg ttgttttgca gagtatacaa aggtgaaaga
    16501 gaggaattta ataaaaatac cagatgagat aagttatgag actgccgctg cgctagaacc
    16561 agtatgtata gcagggcatg gtctttttag atcagaagct aaagttggag atactgttgt
    16621 agtacttggt acaggtacaa taggattatt ttcaatacaa tgggcaaaga tatttggctc
    16681 tacaaaaata atagctgtag atgtatttga tgagaagtta gacttggcga aagagttagg
    16741 ggcagatatt tgtataaatg caaaagaaaa gaatattgta gaagaaataa aaaggttaac
    16801 tgatggagat ggtgcagata ttgttattga atcggctggg actccaatga catgtgggca
    16861 agtattgttg ttagctaaaa agggagggac agtattgtat gcaggtgtac catatgggga
    16921 tgtagctttg actagagaac aatttgaaaa gatagtaaga agtgaattaa ctgtcaaggg
    16981 aacttggttt ggaaactctt ttccattccc aggaaaagaa tggagtgctg gattgtatca
    17041 tatgcaaaag ggtgatatga atgttgaaaa attagtaaca catagaataa atttagaaga
    17101 agtaccagct tattttgaaa aagtatacaa aagggatatt ttctttggta agataatgat
    17161 taatatagat aattagatta gagtactaaa taataatcca tataaacaag gttaagaaag
    17221 gtatgattca tatgacaggg ataattatag ccacacatgg aaatatggct aaagcaatgt
    17281 tagaaagtgc tgaattaata gtaggcaaac aggaaaatgt agaaacttta ggattaaacc
    17341 atggtgacag catagatgag tttagtagaa ggttagaaga tagaattgag aaatataagg
    17401 atgaaggggt tttagtattg cttgattttt atggcgggac tccatttaat acatctgcta
    17461 ttataataaa taaatttgct aaggtatgtg agttagaatg tttgacaggt gtaaatttac
    17521 ctatgctttt agagctattt ttaaacagag atactatgaa gttaaaagac ttgaagaatt
    17581 tatgtgagga agttgggata tcaggaatca aagatgttaa aactgtttta aagttgtaaa
    17641 ataaattaaa aatctggagg aataattatg agtaatatag cattaacaag aattgatgat
    17701 agattaatac atgggcaagt tattactgct tggtgtaaga ttacatcggc aaaaaggata
    17761 gtaatcgtgg atgatttagt agtaaaggac ccatttatag ttcaagtttt acaaatggca
    17821 gctccatcaa ctgttaaggt tgaagtacat gatgtagaat caggtgctga agttttaaaa
    17881 tcacataatg gagatgaaca tttaatagta ttggtcaaat accctaaaac tgtcttaggt
    17941 ttagtaaata gtggggtaga tttaaaggaa ttgaatgttg gaggtatggg agcagggctt
    18001 ggtagaaaaa gtttttataa aaatatatct gtgtcagatg aagaaaaaga aatatttaga
    18061 gaactaatat caaaagatgt taaatgcttt atacaaatag taccagatgc aaagaaaatt
    18121 gatgtaggaa cattattata aaaaagacta tcttgtaaat ctttaaaaga aaatagggag
    18181 gaagaatatg cacatttcac taattcaagc agtattaata gcaatttttt attatctttc
    18241 ttggagtccg tggttgacct atgttggatt ttttacatgg aatagacctc tattggctgg
    18301 atttgtaacg ggaattatat taggagaccc tgttcaagga gcaattatag gagcaggtat
    18361 aaatatgata tatctaggtt ttatatcagc agggggagct cagatggggg acccagcttt
    18421 tgcaggatat gtaggtactg ctttagctat agcatctaag ctagatgtaa gtactgcaat
    18481 ggcaatagct gtaccattgg gaactgttgc aactgtactt tggattggaa aaatgacagt
    18541 aaattctttt tttgcacatt gggctgatag agaggttcaa aaagggaatg ttgatagagt
    18601 agcatttata aatatagtac caccacagat actgttattt gcaatgagtt ttataccagc
    18661 attattagtt gtttattttg gtccaaatgc gatagatggt atgttggaag taatgaatga
    18721 gaatgtgtta catgtattta atgtaattgg ggctatgttg ccagcacttg ggattgctat
    18781 gaatttaaaa ttgattggaa acaagtttac tatgcccttt ttcatattag gaatactaat
    18841 ggctgtttat tttaaagtag atataatagt aatatctgtt attggagtaa tacttgctct
    18901 tacaattact tcaattaagt acggaaaaga tagtgctaca tcttaaatac atgtgagggg
    18961 gaaaatatta tgtcagatat aaaaaatgaa gtatctaaga aaacgttaag caaaaaagat
    19021 gtaataaaat catggttaag atggtttttc tttgcgcaat ctaattacaa ttatgaaaga
    19081 ttgcaatcaa cagctttctc acattcaatg ttgccagtac ttaaaaaatt atatccagat
    19141 aaggaagagt taaaacaaga agtggagact catttagcat tttttaacac agaacctata
    19201 tgtggatgtg ttatacatgg aataacaata gctatggaag aagaaaaagc taatggagca
    19261 gatgaaataa gtggagatgg tatgaatgct atcaaaacag gcttgatggg tccactagca
    19321 ggaattggtg atactttaac tcaaggagtt ataactccta tagtgttagc tgtttgtatt
    19381 ggtttaactg agggaggagc atcaattgca ggtcctatat tatttgtaat agctcaatat
    19441 ataataatga cttcaatttc ttttggaatg tggacaaatg gatataagta tgggaaaaaa
    19501 gctgttgaaa gtatacttca aggtggaata gtaaataagg ttatagaagg ggcttctata
    19561 ttaggtactt tagtcatggg gggacttgta ggtaggttta ttaatttatc tacaccaata
    19621 agttatacta gtggtgattt taaatttagt cttcaaacgg atttacttga taagatattt
    19681 ccaggattaa ttcctttggt acttacttta ttagtactct ttgctttaaa aaaaggcctt
    19741 tctccaataa aaataatgtt tatactgata gtagtgggag ctattacagg tattttggga
    19801 ctattttaaa taaatatata tgttggtgtg taaattagga ttctatatct attgattata
    19861 tttgtaagat gttggaaaat ttatattaaa aagtttagca attagggtaa ttttataata
    19921 gattaaattt taaacttata taagctatat atagtattta atattttaaa aagtatctca
    19981 gaaagatttt taatcagaat gaggtgcttt
//
LOCUS       DAEPLF010000051        19872 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEPLF010000051 DAEPLF010000000
VERSION     DAEPLF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.051"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            273..1646
                     /locus_tag="KR314_001618"
     CDS             273..1646
                     /locus_tag="KR314_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0328007.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILANSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIEKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDILLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEGKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1902..2969
                     /locus_tag="KR314_001619"
     CDS             1902..2969
                     /locus_tag="KR314_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBH0328008.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGDVLIEMEHPQQIIPEVE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            3066..3374
                     /locus_tag="KR314_001620"
     CDS             3066..3374
                     /locus_tag="KR314_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBH0328009.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3367..4797
                     /locus_tag="KR314_001621"
     CDS             3367..4797
                     /locus_tag="KR314_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBH0328010.1"
                     /translation="MNNSTSIKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGAKIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4875..5249
                     /locus_tag="KR314_001622"
     CDS             4875..5249
                     /locus_tag="KR314_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBH0328011.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWNLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5243..7447
                     /locus_tag="KR314_001623"
     CDS             5243..7447
                     /locus_tag="KR314_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBH0328012.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDVAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLRIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7447..8856
                     /locus_tag="KR314_001624"
     CDS             7447..8856
                     /locus_tag="KR314_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBH0328013.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELYIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8841..9905
                     /locus_tag="KR314_001625"
     CDS             8841..9905
                     /locus_tag="KR314_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBH0328014.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERSIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKKLQDINLPKILIRIPMLSELEEVINYCDISFNSELETI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9935..11356
                     /gene="nhaC"
                     /locus_tag="KR314_001626"
     CDS             9935..11356
                     /gene="nhaC"
                     /locus_tag="KR314_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBH0328015.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11785..12249
                     /locus_tag="KR314_001627"
     CDS             11785..12249
                     /locus_tag="KR314_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBH0328016.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12547..12909
                     /locus_tag="KR314_001628"
     CDS             12547..12909
                     /locus_tag="KR314_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0328017.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            13504..14364
                     /locus_tag="KR314_001629"
     CDS             13504..14364
                     /locus_tag="KR314_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0328018.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIIANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            14622..16736
                     /locus_tag="KR314_001630"
     CDS             14622..16736
                     /locus_tag="KR314_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0328019.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEHDVELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYVGTLTQLISM"
     gene            complement(16998..19241)
                     /locus_tag="KR314_001631"
     CDS             complement(16998..19241)
                     /locus_tag="KR314_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0328020.1"
                     /translation="MSNIIIKGLTQNNLKNISFKIPKNKIVVFTGVSGSGKSSIVFDT
                     IAAESQRQMNETYSSFIRNRLPKYRKPQVELIDNLTASVIVDQTPLGGNARSTVGTIS
                     DLYSSLRLLFSRIGEPYIGTTSYFSFNDPNGMCKNCSGIGRVKVINIESIINPENSWN
                     TGCINDSLFKPNSWYWKQYAESGLFDLDKPIKDYSFEEYNLLLYGSKNKDGKQENPKI
                     EGIFNKYNQTYLNRDISNMSKHTKEKSERLITDKECPVCHGKRLNKETLNCNILGYSI
                     ADMCNMELTDLYDLLLQIKDKRTETVIKTLTDGLKRMIDIGLPYLHLNRESSSLSGGE
                     AQRLKLVRYMGSSLTGMTYIFDEPSAGMHPRDVHRMNQLLKKLRDRGNTVLVVEHDKD
                     VISIADEVIDIGPSAGTQGGHVVFQGSYDKLLLANTLTSNAMNLSLPIKTDSRHPKGT
                     LPIRDACINNLKHIDVDLPVGIMTVVTGVAGSGKSTLISRVFAQKYEKDVIKIDQSPV
                     TATNRSMPATFLGFFDEIRKVFAKENNVEEGLFSFNSKGACPVCDGKGVLVTELVFMD
                     PIINTCEACKGTRYSEEVLSKLYKGKCIVDVLSMTVDESLEFFTEKKLCKILQTMKDV
                     GLPYMTLGQPLSTLSGGERQRIKLAKNLNKKGTIYILDEPTTGLHGSDIEKLMLIFEH
                     IVDKGNTVIIIEHNLDVLKQADWVIDIGPDGGKNGGQVVFEGTPLEMFERGCTITSDF
                     LRKSINI"
     gene            19573..>19872
                     /locus_tag="KR314_001632"
     CDS             19573..>19872
                     /locus_tag="KR314_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328021.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKDLNKRKMIGCIFSIFI"
BASE COUNT         7706 a         2312 c         3553 g         6301 t
ORIGIN      
        1 ttagtttgta tatctctttt tttaataaaa tcaataatat ataaaactat tggcatattt
       61 aatgacaatt caaatattgt atataaccat aatgaaagat atataaataa aattttaaat
      121 atacttaaaa atatattaat ataatgtaca taaaaaatat cataaatata tgataagata
      181 aatacaatag caatatttta ttaatttttt gggtttttat atatttaggt actggggtga
      241 tatagttgaa ccaagagata ttttttaaaa taatgaatga aatattggaa aatattgatg
      301 aaggggtaca ttttgttaat aaagacggga aaactataat ctacaataat tctgtacaaa
      361 aaatggaaaa aatgaacaga cacgatattt tagccaattc attttttgat atagtcaaca
      421 aaatgaaaat aaacaggagt acactattag aagttttgga aaaaagggaa gttatcaaag
      481 acaatattca aaaatatctg gacaaaaatg gaaaagagat aacagcaatt aatactacaa
      541 taccaattga tattaaaggc gagtttgtag gggcacttga aatatcaaag aatatgacaa
      601 cgatagaaaa tttactaaac agaaattata aaaaaaattt agtcaataaa gttagtagta
      661 gacaaaaaaa gggttatgaa tttgatgata ttttagggga aagtaatttg attaaagaag
      721 caatagaaaa atctaagaaa gcttgcaagt ctgatgcttc agtatttata tacggtgaaa
      781 ctggaacggg taaagagtta tttagccagt ccattcatta tggaagtaat aggagaaact
      841 atccatttat agcacaaaat tgtgcggctt tgccggaatc actattcgaa ggtatccttt
      901 ttggaactag caagggagga tttacaggag caatagatag gccaggctta tttgaacaag
      961 ctaacaaagg gacattgtta ttggatgaga taaattccat gcctattatg ttacaggcta
     1021 aactattaag ggttttacaa gaaggttatg taagaagggt tggaggaact aaggatatac
     1081 ctatagatgt aagaattatt gcaacgacaa atgaaagtcc aaaggtaatt ttaaacgagc
     1141 ataaaatgcg aaaagattta tattataggc ttaacataat tcatataaaa attcctcctc
     1201 ttagagatag aaaagaggac atacttctat tgtgcaaaaa atttatttca aagtataata
     1261 aaaagttaaa taaaaaagtg gtaggaattt cagatgaagc tattaaaatt ttaaaaaatt
     1321 acgattggca aggtaacata agagagctag aaaacataat ttattgtact atgagtatga
     1381 tggaagatga ggtacaaata gaaacaagta tgattaatct aaacgactat tacaatctta
     1441 aagagaataa agagagtaac aattattctt taaacgattt ggagggcaaa acacttagta
     1501 agattatcag tgagatagaa gaaaagtata tatatgaatc attagaaaag gcatcgtaca
     1561 atataactaa agctgcatca attttaggta tgaataggca aaatttacag tataaaataa
     1621 aaaaatataa tatcaaaata ttataaataa gcaagataaa aaaacaaaat agaagtaaaa
     1681 taaaaaagca aaatatagga gcaaaattat tataaaagag caaaatattt tgctcttttt
     1741 tttatttctc ataagaaata ttatttttta tttatgaaaa tacataataa aataattata
     1801 gtttcaacgt tccaataaat atttacttta attttaaagt tggcacgatt tatgctttaa
     1861 ctaattattg agaatatata aatttttagg agggtgtata tatgagaaaa gtaagagtag
     1921 gaatatgggg atttggagct atgggaatag gtatggctaa tatgattcta aagaaagaag
     1981 gtatagaaat agtatctgtt tgtagtagaa gtacttcagg gaaaagtatg tatgatgttt
     2041 taggaattga aagaggagaa agaccagaag ttattatcaa taaaaattat gaagaagttt
     2101 ttagagaaaa aagtgtagat gttgttttgt tagcaactga ttcatttact aaaaaggctt
     2161 ttgataaaat aattttctta ttaaatagaa aaataaatgt tatatcaaca gcagaacaaa
     2221 tggcatatcc acaagctgat gatgcagatt tagctaaaaa aatggatgaa gttgcaaagg
     2281 aaaatggagt aagtatactt ggaacaggaa taaatccagg atttgtatta gatttactag
     2341 tattggcact ttcaggtact tgtgaagaag ttactagtat aaaagctaag agagtaaatg
     2401 atttatctcc atttggaaaa tctgttatgg aggaacaagg tgttggagta acaagggaag
     2461 aatttataaa aggtgtagaa gataagacta tagcaggtca tgtaggtttt gtagaatcaa
     2521 taaatatgat agctgatgga ttaggttgga aattagataa gatagaacaa acaaaagagc
     2581 caataatgac tacagtagat agaaaatcta aatacggaga ggcattggct ggaaatgttg
     2641 ctggttgtag acaatgtgga tatggatatg taaatggaga cgttttaatt gaaatggagc
     2701 atccacaaca gataatacca gaagtagaag gtattaaaac aggagactat gtatcaataa
     2761 aaggtatacc aaacattgat ttacaaataa atcctgaaat acctggagga gtaggtactt
     2821 atgcaatgat agtaaactct attccattaa taataaatgc tagaccaggt cttaaaacta
     2881 tgttggatat accagtgcct agagcaataa tgggagacat aagaaatcag atagaagttg
     2941 aattggaaga agaatcaaag gctaattagt aagataacag ttagctaatt ggtaaaataa
     3001 tgtttaattt aaaataacag taaattaatt agtaaggaat agtcaattaa taaggggatg
     3061 aaaaaatgga tgctaaaatt aatgattggg ttattataca caatatagta ttgactccag
     3121 aagaaagagc tccacaagtt ccagaggata ctaagaaagt atccttagaa atgtgggtta
     3181 aaggttttat acaaagtgat gcttctatag gcgacttagt agaagttaaa acaattacag
     3241 gtagacttgt aaaaggtaat ttacttaaag taaatccata ttatactcat gattatggta
     3301 aatgtatacc agagttactc caaataggaa tccaagctaa agaaatatta tttggaggtg
     3361 tatataatga ataattcaac atcaataaaa gatatgagtt atcaagctgt tatgggaaga
     3421 aataacgaaa taatgaaaaa tgcaatcgga ttggattatt cttcttttga acaagaggga
     3481 atagggtttg attatgagaa aatgatgagt gaaacaggat atacattaca agatattgaa
     3541 gcaattcaat cacagtatgc tgttggaaat actcctctaa tagagcttaa aaatttgact
     3601 aaattagcta gaaaatgtgc taaagaaggt aaaggagcta aaatatttgt aaaagatgaa
     3661 gcaatgaatg catctggtag ttttaaagca agaagagcag ctactgcagt atatcatgcc
     3721 aaacaaatgg gatataaagg tgtaatagca gctaccagtg gtaactatgg tgcagctgta
     3781 gcatcacaag cggcaatgca gggattaaaa tgtataatag ttcaagaatg ttatgattct
     3841 aatggtgtag gtcaacctga aattatagaa aaagcacgaa aatgtgaggc atatggagca
     3901 gaagttgttc agttaactgt tggaccagag ttattttata cgttcttagt tttattagaa
     3961 gaaacaggct attttaacgc atcattatat tcaccatttg gaattgcagg tgtagaaact
     4021 ttaggttatg agttagctat ccaatttaga gaaaagtata aaaaagaccc tgatatagtt
     4081 gtttgtacaa atgcaggtgg tggtaatctt actggaactg caagaggatt gataaaagct
     4141 ggttccatta atactaaagt agtaggagca agtgtagatt taaaaggatt acacatggct
     4201 agtgataatc aatttaataa aaaatcattt acaacaggac atacaggttt tgggatacca
     4261 tactgtacat ggcctgatag gtcagatgta cctaggtctg cagctagacc acttagatat
     4321 atggatagat atgtattagt aaaacaagga gaagtttttt atacaactga attattggca
     4381 cagctagaag gtatagaaag aggtccagca ggaaatacat ctttagcagc agcattctct
     4441 ttagctcaag agttagatga ggataaagtt atagtggtac aagaaacaga atatacaggc
     4501 gcaggtaaac atatatgtcc acagcttact tttgcaagag aaaatggaat agatataaaa
     4561 tttggaaatc caagagaaga aattgcagga gtaaacttaa tattaccaga aagaccagaa
     4621 ttattgaaat gtgttgatat tgatatgaat aaaataagaa agtcatttat aaaaaattgc
     4681 atatctaata atcacattga taatattgat aagctaagta atcgagatat agaattttta
     4741 atggaagaag taaaaagttc aagagatttt gttgttgatg tattaaataa tttatagtaa
     4801 ttattgtttt tttatttaat ataaaataaa aattcggaaa attctattaa atcaatgcga
     4861 atggaggaat gttcatgaaa aaaagagagg atgattttga agttagaaga aaacatcttc
     4921 aagaactgag tgatgatgag ttaaaagaaa ggttttggaa tttagctacc cagatagtag
     4981 aaccaatgat agaacttggt aaaaagaata caacaccatc aatagaaagg tctgtcttgt
     5041 tgagaatggg attttcttct cttgaagtaa aacctatttt agaaggggtt atggaaagag
     5101 gtcttatagg taaaggagca gggcatgtag tttataaatt ggctaaatct aaaaatataa
     5161 cggttagaga agcaggtttg ttattagtta agggtgaata ttgggatgaa gttacatgtt
     5221 tatttagaga aggggtagaa tcatgttaaa ggaaaataaa aaattggata tagactatat
     5281 attaaaagat ttggataaat ataaaccaaa acgaagaggt tgggtttgga gagaacactt
     5341 ggaaaatctt gaaatgggac cttttaaata taaagactgt acaaaacctt taaagaagag
     5401 tgtagggcta ccatcatcta agtatttcaa taatatagac cctcaaccaa gtccagttat
     5461 tactactgaa atagcttctg gtagatttga ggatgatata agaagaatga gaatggcagc
     5521 tcatcatgga gctgaccatc taatggttat cagaactgct ggacaatcac attttgatgg
     5581 gcttatagaa ggaactccac aaggtatagg tggagttcca ataacaagaa aacaagtaag
     5641 agctcaaagg aaggctcttg attttataga agaagaagtt ggtagaccta ttaattacca
     5701 ttcatatgta agtggtgttg ctggtcctga agtagcagta atgtttgctg aggaaggagt
     5761 caatggagca catcaagacc cacaatataa tgttctgtat aggaatataa acatggtaag
     5821 gtcttttgta gatgcttgtg aggcaaagaa aataatggct tttgccaata tagctcaaat
     5881 tgatgtagca cataatgcta atgctactgc tagagaagca tggaaggtaa tgcctgaact
     5941 tatggttcaa cacgctttaa actctatatt ctcagaaaaa ataggtatag ataaaagtaa
     6001 tatatgctta tcaacagttc caccaacagc acctccagca ccatgtttaa gaatagactt
     6061 accttacgct gttgcactta gagaattatt tagtgattat aagatgagag ctcaaatgaa
     6121 tactaaatat atggaatcat caactagaga agcaacagtt actcatgtat taaacttatt
     6181 aacatcagta ttaactagag ctgacataca atcaactata acacctgatg aaggtagaaa
     6241 tgtaccttgg catatataca atatagaagc atgtgataca gcaaagcaag ctttagttgg
     6301 tatggatggg cttatggata tgatagaact taaagatgtc ggtgaattaa gagacaaagc
     6361 aagagaactt aaagaaagag ctgtattata tatggaagaa atatctgaag ttggtggata
     6421 ttttgaatct gtggaacaag gattttttgt tgattctgga aattatccag aaagaaatgg
     6481 tgatggaata tctagaaaaa taaaaggtgg agttggtgaa ggtactgttt atgaaagaga
     6541 agaagattat ttagcacctg taactgctca ctttggatac aataatgtag ctcaatatga
     6601 tgaaaatgct atagataatc catcaatgtt aatagatgga tgtacatttg aaaatccaga
     6661 caaaataata tatattgatg agcttgacga ttttgataat gtagaaaata gactaaaaga
     6721 atcatatgag tacagaaatg gaactaaaat taagcctgaa atggaatggt gtgcagatgg
     6781 aattgtaatg ataacaatga tgcttcctac tgataaaaga acagcagagt ttgcagcttt
     6841 ggaatttgtc aaaaaaatga acttacaaga aattgaggtc ataagtagag aagttatgca
     6901 tgaatctgaa gggactagaa tagaagtgaa aggaagagtt ccgtttgata tagatttaaa
     6961 caatttagta ataccagaag aacctaaggt actaactgat gaagaaataa gagcagatat
     7021 agaagaaaaa cctatgaaaa tagtttcagc tacggttgga gaagatgaac attcagttgg
     7081 tttaagagaa ataatagata taaaacatgg aggtattgaa aaatatggca tagaatgcca
     7141 ctatctaggg acttctgtac ctgtagaaaa attggtagat gcagcgattg aactgaatgc
     7201 agatgcaata cttgcatcta caataataag tcatgatgat attcactata aaaacatgaa
     7261 aaaattacac gattattgtg tagaaaaagg tataagagat aaagtaatga ttgcgtgtgg
     7321 aggaacacaa gtaacacctg aaatagctgt tgaacaaggc atcgatgctg gatttggaag
     7381 aaacagcaaa ggcatacatg tagccacttt cttagttgaa aaaagaagag aaatgagtaa
     7441 taaataatgg aaaatagcaa agtttcttta aaaatagatg tgttggtagc tgaaattgga
     7501 agtactacta cagtggtaaa cgctttccac gatattaata ctaacaatcc tatatttttg
     7561 ggtcaaggtc aagctccaac aacagtgttt gaaggtggcg atgtaagaaa tggcttatca
     7621 ggtgctatta aagatttagc taataaatta tctgtagatg atatagagta taatgatatg
     7681 tttgcaactt ctagtgcagc tggtggatta aaaatgactg ttcatggatt agtctatgat
     7741 atgacagtaa aagcagctaa agaagcagct ttaggggctg gagctgttat tcgtcaaata
     7801 acatcaggta gaattaaaag aactgattta aataaaataa aagaaataaa cccaaacata
     7861 attttaatag ctggtggagt cgattatgga gaaagagata cagctattta taatgcagaa
     7921 atgatagctt ctatgaatct aggcatacca gttatatatg ctggtaatgt tgaaaatcaa
     7981 gaggaaataa ggttaatatt tgaagatact aattataagc tttatataac tgaaaatgta
     8041 taccctaaaa tagatttatt gaatatagaa cctacaagaa gaatcattca aagtgtattt
     8101 gaagaacaca taactacagc accaggaatg aaatacatta aagaaatggt taatgaaaat
     8161 attactccaa ccccaggagc tgtaatggaa gcttctaaac ttttatataa aaatattgga
     8221 gatttattaa ctttggatgt aggtggagca actactgatg tacattctgt tacagatgga
     8281 agtgactata ttaataaaat tttggttaat cctgaaccta ctgcaaagag aagtgttgag
     8341 ggggatttag gtgtatatgt aaatatgaag aatatagtag aagttatagg taaagaaaac
     8401 ttacaaagtg aactatatat agatattgat gcagtaatag agaattatcc tccaatacca
     8461 aaatcaaaag aagaaatatt atttgttgag aggttgacaa aagaagctgt tgttaaagct
     8521 atgctaagac attcaggaaa aataagaaat atctatggaa caactggaaa agtcaaaatt
     8581 gcagaaggga aagatttaac agaagtaaga tatatagttg gtacaggggg agcacttact
     8641 aggctcccta gtagaattga aatattagat aatatgctca aatacaataa aaataatgaa
     8701 ttactatttc caaaggaaaa aacaaagata ttaatagata atgactatat tatggcatct
     8761 atgggtgttt tatctaaaaa atatgaagaa gcatctctta agctcctgtt aaaaagtttg
     8821 aattttgagg aggaaagatt atgtatccta ggttagaaat agatatagaa aagttaaagc
     8881 ataatgcaaa attaatttcg cagatgtgtc atgaaagaag tattaagata tcttttgtta
     8941 ctaaatcatt ttgtgcacaa aaagaaatag tagaagaaat atgtagtgaa ggaatagacc
     9001 atatagcaga ttcaagaata caaaacttaa aaaaattaca agatataaat ctacctaaga
     9061 tattgattag aatcccaatg ttaagtgaat tagaagaagt tatcaattat tgcgatatta
     9121 gttttaactc agaattagaa acaataaaga aattaaatga attatgtgaa agtaaaaata
     9181 ttattcataa gatagttctt atgtttgact taggcgattt acgagaagga tacttttatg
     9241 aagaagactt ttttaataat gtaagagaga tagtaagatt aaaaaatatt gaaataattg
     9301 gtatagctac aaacttaacc tgttatggag ctattatacc atctagggaa aatataggca
     9361 gactggtaag tatagcaaaa agaatggaag aaaaatttgg aataaatcta gaaatagtat
     9421 ctggaggtaa ttcaagttct attcatctgt tgataaataa taatatgcca gaaggtatta
     9481 ctaatttgag aataggcgag tccattctac ttggtagaga aacggcatac ggtgaaaata
     9541 taaatggaac atatcaagat gcatttaaat taatatgcca agtagttgaa tgtaaagaaa
     9601 aaccatcagt tcctattgga gaaataggag ttgatgcctt tagaaataaa cctgtttatg
     9661 aagataaggg aatcttaaaa agggcaatca tagctatagg taaacaagat ataaatattg
     9721 actctttaat tccaatagac actgatataa aaatattagg ggcaagttca gaccacatga
     9781 tactagatgt aagtaacaca aaatatgatt ataaattagg tgacaattta gaatttttat
     9841 taacttatgg gggtatcatg tcatctagta caagcaaata tgtttcaaaa aaaattatag
     9901 tgtagtgttt aaataattag gaggaaacta ttttatgaaa gagcgagaat gtagaaaagc
     9961 aagtctgttt gatgcaatga taccaattac ttgtttgata ttatttttaa gtctaggagt
    10021 attagtgtat ggttcatcac ctcatgtacc actaatagga gcagcagcag tagcaggact
    10081 tgtggctgta tatagacttg gatttaaatg gaaagagtta gaattaagta tgtttaatag
    10141 tataaagatg gcaatgcaag caatcttaat cataattata ataggcgtac ttataggaac
    10201 ttggatagta agtggagtag ttccatgtat gatttactgg ggactaaaaa ttttatcacc
    10261 aaatattttt ttagtagcat caacattagt ctgtgcaata gtatctcttg cgacaggttc
    10321 atcttggagc acaatgggta cagtaggagt agctctttta ggaattggtc aaagtttagg
    10381 tatgccaatt ggattaatcg taggttctat aatatctgga gcttactttg gagataaact
    10441 atcaccttta tctgaaacga ctaacttagc tccagcaatg gcaggaacag atttgtttac
    10501 acatataaaa tatatgttat attcgactat accatcatta ttaatttgtt tagtaattta
    10561 tggtgtaatt ggaatgaagt attcaggtca agcactagat ataaaacaaa tagaattaat
    10621 taggtctaca ttagattcta catttaatac tttatcacca gtattattat tagctccagc
    10681 aatagtaatt ggattagttg tatttaaagt tcctgctata ccagggctta tagtaggtgc
    10741 tgtattggga gtattgtttg cagtagtttt tcaaggagaa tctatgaata cgatattaga
    10801 tgcagcacaa aatggatatg tttcgtcaac acaaatacag gaggtagatg ctttattatc
    10861 taaaggtggg attatgaata tgatgtccac tgtatcctta acaatatgcg ctttatctct
    10921 tggaggtatt ttagaaaaaa caggtatgct tgaagtagta gcatcttctt tactaagatt
    10981 agcaaagggt gtatttggaa cagtattatg tactatggta acttgtacaa ttaccaatat
    11041 aatagcaggt gaacaatatc tatctatagt tataccagga agaatgtata ataaggaata
    11101 taaaaagaga ggtatacatc ctaagatgtt gtcgagagct ttagaagata gcggaacttt
    11161 aacttctcca ttagtaccat ggaatacatg cggggcgtat ataacagcaa cattaggtgt
    11221 atctgcacta acatatggtc catacgcttt attaaatatt ataaatccaa tagtatcttt
    11281 agtattaatt gcatgtaagt ttaaaatagc aagaatagaa gatgaacctg aaacttgttt
    11341 aaattttgat gtttgataat agataactta tgtaagaacg taggaactag ggggggagcc
    11401 tgtggggaat tagtaagaca tattttagtg tgcgaaattc tataaatcag taatcttgtg
    11461 actttagcca tgggaggttc agattagtaa atttatagtt ttatattagt ctatactttg
    11521 atcttttaat tgtttgtata aattttttat agaaaaagat ttcttttaga cttgcctaaa
    11581 aattaggcaa gtcttttaag tatgttctga ttataagagc actgtgtgac aagaatgatg
    11641 agattatttt tgctatactt tcgatataag aaatggaagg aggttatgtt ttatatttat
    11701 atgaaataat tagttacaca gtgaattttc gtataatttg gtataaaaag attagaaatg
    11761 aaaataaagt aatggaggaa taaaatgact agaatatcca atataacaat agctgaacaa
    11821 tctgagtact gttttttggc aatacgaaaa acaattgact ttatggtaga gttttcagag
    11881 ttttcaaaac aaagttttga aaaaatttct aaatatctgg aggagagagg aattttatct
    11941 tcaggagcac cgattgtatg ttttcataac atggatttag caaaattgga tgttgaagta
    12001 gggtttcctg tagccactta tatagatggg aaaaatgaga ttctacaaag gattgttcct
    12061 gcacagaaaa taataactgc aattgattta ggaccttatg aattacaaga ccctacttta
    12121 gaagacttat tttcatgggc aaatagcaat ggatataaac ttcatggcga tatatattac
    12181 cagtatttaa atgatatcaa tcgtccagct agtgaatatc ttacgaaaat gatgttaccc
    12241 attatataga aagttaaaga ttatcgtttg ttagttgata attagaagaa catttaatgc
    12301 tactgcataa attttatcct aaataatatc aaggtgattt aatttatgta ttctaatgta
    12361 actgtagata atcaatttat aactaactta gtgtaattta gatttaattt attattctaa
    12421 tgtaacccca atatattgtt aaggtctact taagatgctc aaaaggctat gcttaattta
    12481 ttgttatata atttgtagag tggtataatt tagaagtgat actatataga tatatagggg
    12541 gaaaatatga aaatatatga tatattaaca cgtttttata ttcaagatat agaaaaagcc
    12601 ataccttttt atgagaatct tttaaaagaa aaatgttctt tgcgtttttc ttataaagaa
    12661 gttgggttgg aattagcgca aataggaaat gttttactat tatctggttc tgatgatgct
    12721 cttaaaccat ttatagaaac aaaatctact tttatggttg attctgttga tgaatggaga
    12781 agttatctat tggataatgg agcagttgta gtaagagata agaaaaaagt tcctacagga
    12841 tataatatga cattgagaca tccagatggt acaattatag aatatgttca acatacaaag
    12901 caggattagt taaaaataga ttttactact gagcaactaa ttagaatcta ttcttagacg
    12961 tacaatttat tatttttggt attaatagat tataagtcaa attataatat ttcaaaatag
    13021 aatagaaatt tatttacata aaagagagtg tttaaaaatg aactttttag ttcacgaagc
    13081 actccttttt gtatgaaatc aaatacatca atatataaaa caaactgatg aagagcttaa
    13141 attaaaataa aaagattcat aacaggaccc cattaaactt taagataggg taagtttgta
    13201 atcatattaa ataattgtaa aaagctgttt ttagtcgtat aatataaata tttatcattt
    13261 aattaaggaa tctaaaagga atctaataag aagaaaataa taaataactt tttagattta
    13321 ctaaatgaaa aattattgaa aattaaagga ttagagtctt taatcagaaa gtaaaaagta
    13381 atatattaaa tttatgaatt gaaaacttaa acaacaaatt tgtttataaa atagcaagat
    13441 tcattaaata taactaagta ataattgata taatgttgca tggataaaag gagggaagct
    13501 gcaatgtctt acaaagtaat taatgtcata actgctattg actatataga ggagcattta
    13561 tcagaaaagc tggatttaga tataattgct aatgctgttc attactcaaa atatcacctg
    13621 catagaactt tcacaacttc tgttggtcta acaatgcatg attacataaa gcggagaaag
    13681 ttgacagaag cagcaaaatt attggtattt tcaaagaaac caattattga aattgcattg
    13741 atagctggat atgaaagtca acaggcattc acttctatat ttaaagctat gtataaaaaa
    13801 tctccaaata aatataggaa agaacaagaa ttttatcctt tgcagctaag atttgttttg
    13861 aaaaaagata acctattttc aaagaatgta ctagagttgg aaaaagaaat taaacttgca
    13921 agtatgtctg acataccatt atggatgaat ttagtgagat tagtcataga tggatttcca
    13981 aatttgcaag aggaagaata tatatatcaa ttagaacagt atattttaga agaaagagct
    14041 ttaatattaa aacttaataa tgtcgctatt gcaaatatgg tgtttaatag agaaactaga
    14101 agtattgatt tttttggaat acatccacag tatcgtaatt cagacattgc acaagtgttt
    14161 ttgaagaaag ttattgaaga tttcttgata gatacagata tctcaattac tacctttcga
    14221 gagggtgaca aagctgatac aggacataga gatatgataa agaagttggg gtttgcagaa
    14281 gcagagttgc ttgtggaatt tggatatcct actcaaaaat ttattcttcc ttgtgtagaa
    14341 gaaagagata atagacaaaa ttaattgtag attaaatgca aagtttacca catatttttt
    14401 gtttgaatta ttttatatat aagagtattt ctgcttttaa tgtattttgc ttgtaagtgt
    14461 acttatggta ataaatatag aaagatttag ttttttaaaa aaagaacttt aaagctaaaa
    14521 agctaacatt taagttaaat agcaagaatg attaaaaact ccaaaacaca attaagtata
    14581 atgattcagt gtaagtactt aattaacaaa ggaggattta aatgtctgaa aataaacaag
    14641 tagtaaattg cgatattatt gcatccaatt ctaaacataa tatgtgtgaa catgatgtag
    14701 agttattaac tttttttcca gatgagattg cacatctaat agagataaat aacaagctag
    14761 acaatgcttt taagaaagct gaaaatcttg ttgacaagct tgataaggat tatatggacg
    14821 ctaaaatgta tatggtaaaa aatcgtggtg aaatagaccc acatgaaatg tttcaaaatg
    14881 aacaaggact aaaacaaata gataactatg gggcttttat ggtaaaagtt cgagataaga
    14941 ttaataaaat caaagattct ccatactttg ctagaataga ttttaggctg aaagatatgg
    15001 atgatgaatc taagtattat attggtagat ttgcatttga ttatgaagat gagcttataa
    15061 tacttgactg gcgttctcca attgccagta tgttttatga ttatgaaatc ggaaaagctg
    15121 gatatgatgc tccaatagga tgggtggatg gagaaataac aagaaaacga caatttaaaa
    15181 ttaaaaatgg aaaattggaa tatgcactag aaagttccat taatatacaa gatgatattc
    15241 ttcaaaaaga acttagtcac acttcagatg aaaaaatgaa gtcaatcatt tcaacaatac
    15301 aaaaggaaca aaatcaaatt attagaaatg ataaagctga tacactgatt attcaagggg
    15361 tagctggttc tgggaaaaca tctatagcat tacacagaat tgcttttctt ctttatcgat
    15421 ttaaagataa aatatcagca aataatgtaa ttatcctatc acctaataaa gtctttggag
    15481 actatatttc caatgtcctt cctgagcttg gagaagaacc attatgtgaa ttaagttttg
    15541 aaaatatagc agaggtacaa ttagatagag ttattaattt tgaaagtgag aaagacccat
    15601 tagaaataaa tgatgctaaa tggtctgaaa gagtgcgttt taaatctaca cttgattttg
    15661 taaaacttat tgatgattat ataaaacaaa tgccaaataa gatttttatt ccaaaagact
    15721 acacttttgg aagttttaca gctaaaagtg actggataca gagtagattt gaggcatata
    15781 acagatatcc agttaagaag agattagaaa aggtagctga agacattcat tataaatttg
    15841 aatcagataa tattatggaa gaagatttac caaaggtaaa aagtatactt aaaagtttga
    15901 atggaatgtt gacaataaaa aatactctta cactatataa ggatttcttt aaacaaatga
    15961 atatatcaaa catgtttgtg atggcagaaa agaaaacact tgaatggtca gatgtatacc
    16021 catttatata tattcatgct gcatatgaag gtatacaaga agataagatt ataaggcata
    16081 ttgttattga tgaaatgcag gactatacac ctattcaata tgctgtaata aacttacttt
    16141 ttaagtgtaa aaagactatt cttggagatt ttggacaatt ggttaatcct aatcatactc
    16201 atacattaga tgatatgaaa cagctatata atgatgggga actagttatg ttgaacaaga
    16261 gttaccgttc tacttttgag attattaatt tcgccaaaaa gattcaagat atatcttcat
    16321 tagagcctat tgaacgacat ggtgaagaac cggctttatt aaaatgtaat aacgagcaag
    16381 atgaaataaa taagataaaa atagaaattg aagaatttaa aaagagtgat aatgcgactc
    16441 ttggtattat attaaagaca gataatgacg ctaaagctgt atatgatgcg ttaagagaag
    16501 aatatagtgt tcacttaata tcttctgaaa gttctagctt tacaaaagga gtatccataa
    16561 cttctattaa aatgtcaaaa ggtttagagt ttgatgaagt tattgtacct tcagtaaata
    16621 ataaaacata ttatagtgat tatgaccgca gtttgttata tattgcttgt acacgagcta
    16681 tgcacaaact taagttaaca tatgttggta cattaactca acttataagc atgtagatta
    16741 tatcttatat gcatttatta tcgtaaatat aaattgggac aaagattcat caataaatca
    16801 acaaaaaata atacttataa atttataaaa aatgaaagct ataaattaca tttgaaattt
    16861 tataaaattt tgtgatttat agcttttata atattaattt cttttaaaat aaagttaaac
    16921 tttaaattta tgaaataaaa caaagtagta atatatatgt aatagttctt aattttttaa
    16981 tattagtatt atattatcta tatatttatt gattttctaa ggaaatctga tgttatagtg
    17041 caacctcttt caaacatttc tagtggtgta ccttcaaata cgacctgacc accgtttttg
    17101 ccaccatcag gaccaatatc aattacccag tctgcttgtt tcaatacatc caaattatgt
    17161 tcaattataa ttacagtatt acctttatca actatatgct caaaaatcaa cataagcttt
    17221 tcaatatcag aaccatgaag ccctgtagtt ggctcatcta aaatatatat agtacctttt
    17281 ttgttgaggt ttttagctaa cttaattcgt tgacgttctc ctccagatag tgtgctaagt
    17341 ggttgaccta aggtcatata aggtaaacca acatctttca ttgtttgtaa gattttacat
    17401 agcttctttt ctgtaaaaaa ttcaagagat tcatctactg tcatagatag tacatcaact
    17461 atacatttac ctttatataa ttttgacaaa acttcttcac tatatctagt acctttgcaa
    17521 gcttcacatg tattaataat agggtccatg aatacaagct ctgtaacaag tactccttta
    17581 ccatcacaaa caggacaagc tccctttgaa ttaaagctga ataaaccttc ttcaacatta
    17641 ttttctttag caaatacttt tcttatctca tcaaagaaac caagaaatgt agcaggcata
    17701 gaacgatttg tagctgtaac tgggctttgg tcgattttaa taacatcttt ttcgtatttt
    17761 tgtgcaaaaa cacgagagat taaagtactc ttgccagaac ctgctactcc agtaacgaca
    17821 gtcataattc caactggtaa atctacatca atatgtttta gattatttat gcaagcatct
    17881 ctaattggaa gggtaccttt tggatgacga gagtcagttt ttataggtaa tgatagattc
    17941 attgcatttg aagtaagagt gtttgctaaa agtaatttat cataagagcc ttggaataca
    18001 acatgtccac cctgagtacc agcacttggg ccaatatcta taacttcatc tgctatggag
    18061 ataacatctt tatcatgctc tactacaagg acagtatttc ctctatctct aagttttttt
    18121 aatagctgat tcatacgatg tacatctcgt ggatgcatac cagcactagg ttcatcgaaa
    18181 atataagtca tacctgtgag actgctcccc atatagcgta caagttttaa tctttgagct
    18241 tctcctccag atagagatga tgattctcta tttagatgta ggtaagggag accaatatca
    18301 atcattcttt ttaaaccatc agtaagagtc tttattacag tttcagttct tttatctttt
    18361 atttgtaaca ataaatcata aaggtcagta agttccatat tgcacatatc tgcaatcgaa
    18421 tatcctaaaa tattacagtt aagagtttct ttatttaatc tttttccatg acatacagga
    18481 cattccttat ctgttataag tctttctgat ttttcttttg tgtgtttgct catattgcta
    18541 atatctcgat ttagatatgt ttgattgtat ttgttaaaaa taccctctat ttttggattt
    18601 tcttgttttc catctttatt ttttgaacca taaagcaata aattatattc ttcaaaagaa
    18661 taatctttta taggtttatc taaatcaaaa agtccagact ctgcatactg cttccagtac
    18721 catgaattag gtttaaataa agagtcattt atacatcctg tattccatga attttcagga
    18781 tttataatag attcaatatt gattacttta actctaccaa tcccagaaca atttttacac
    18841 ataccatttg ggtcattaaa agaaaaatat gaagttgttc ctatgtaggg ttctccaatt
    18901 ctagaaaata ataatcttaa tgatgaatac aaatcactta tagtgccaac tgtagaacgt
    18961 gcattgccac caagaggagt ttggtctacg attacagaag cagttaaatt atcaatcagc
    19021 tccacctgag gttttctata tttaggtagc ctatttctga taaatgaaga atatgtttca
    19081 ttcatctgcc tttgtgattc tgctgcaatt gtatcaaata cgatactgga ttttccagaa
    19141 ccagaaactc cagtaaaaac aacaatttta tttttaggaa ttttaaatga tatatttttt
    19201 aggttatttt gtgtaagtcc ttttattata atatttgaca tgaatgttaa atcctttctg
    19261 agtacatttt agtaaagcaa ttatattatg taaccatatt ataataaaat taaaatctac
    19321 tttcttgata tattctgcta tgttatagaa ggtatttcta aatttaacca atcccataca
    19381 atctgaataa atgatagttg ataatagata aaaaagtata taatagatac tcgtaatttg
    19441 ataagtacaa agataattat acaattaaaa cgacttgaca tttaaaactt actattgtaa
    19501 tatttaaata ttacaatagt aagttttaaa tagagaataa ataattacct cacaaactga
    19561 aaggaggaca taatgatact aatagttata ttgagtatat tcatcatagc ctcaatcaac
    19621 ataattatat taggtaaaga aaatataaga gatatagtta tccctttaac tctatttatt
    19681 gtaagtgatt ttttattttt gtgtggaaat ataatatgta ggggaggagt aatgctacta
    19741 tttataggtt tatttgcaat accattgatg ctaattgcat ttatatggat ttgtgtacat
    19801 atgattaagc tttactataa aaaagattta aataaaagaa aaatgattgg atgtatattt
    19861 agtatcttta ta
//
LOCUS       DAEPLF010000052        19416 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEPLF010000052 DAEPLF010000000
VERSION     DAEPLF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.052"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            234..944
                     /gene="rgaR"
                     /locus_tag="KR314_001633"
     CDS             234..944
                     /gene="rgaR"
                     /locus_tag="KR314_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBH0328022.1"
                     /translation="MFRVVICDDEKIQRSILKEFTKKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
     gene            1990..3021
                     /locus_tag="KR314_001634"
     CDS             1990..3021
                     /locus_tag="KR314_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBH0328023.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            3154..4452
                     /locus_tag="KR314_001635"
     CDS             3154..4452
                     /locus_tag="KR314_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBH0328024.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(4566..4811)
                     /locus_tag="KR314_001636"
     CDS             complement(4566..4811)
                     /locus_tag="KR314_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBH0328025.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            4959..5825
                     /locus_tag="KR314_001637"
     CDS             4959..5825
                     /locus_tag="KR314_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBH0328026.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(5829..6365)
                     /locus_tag="KR314_001638"
     CDS             complement(5829..6365)
                     /locus_tag="KR314_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBH0328027.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(6555..6758)
                     /locus_tag="KR314_001639"
     CDS             complement(6555..6758)
                     /locus_tag="KR314_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBH0328028.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            7049..7813
                     /locus_tag="KR314_001640"
     CDS             7049..7813
                     /locus_tag="KR314_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0328029.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKYDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            8202..9515
                     /gene="prdC"
                     /locus_tag="KR314_001641"
     CDS             8202..9515
                     /gene="prdC"
                     /locus_tag="KR314_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBH0328030.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            9684..9846
                     /locus_tag="KR314_001642"
                     /pseudo
     CDS             9684..9846
                     /locus_tag="KR314_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            10316..12076
                     /gene="prdR"
                     /locus_tag="KR314_001643"
     CDS             10316..12076
                     /gene="prdR"
                     /locus_tag="KR314_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBH0328031.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            12505..14385
                     /gene="prdA"
                     /locus_tag="KR314_001644"
     CDS             12505..14385
                     /gene="prdA"
                     /locus_tag="KR314_001644"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBH0328032.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            14466..14750
                     /locus_tag="KR314_001645"
     CDS             14466..14750
                     /locus_tag="KR314_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328033.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            14788..15513
                     /gene="prdB"
                     /locus_tag="KR314_001646"
     CDS             14788..15513
                     /gene="prdB"
                     /locus_tag="KR314_001646"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:15238..15240,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBH0328034.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            15593..16351
                     /gene="prdD"
                     /locus_tag="KR314_001647"
     CDS             15593..16351
                     /gene="prdD"
                     /locus_tag="KR314_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBH0328035.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            16366..16833
                     /locus_tag="KR314_001648"
     CDS             16366..16833
                     /locus_tag="KR314_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBH0328036.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            16851..17333
                     /locus_tag="KR314_001649"
     CDS             16851..17333
                     /locus_tag="KR314_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBH0328037.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPAVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            17353..18360
                     /locus_tag="KR314_001650"
     CDS             17353..18360
                     /locus_tag="KR314_001650"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBH0328038.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            18480..19361
                     /locus_tag="KR314_001651"
     CDS             18480..19361
                     /locus_tag="KR314_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBH0328039.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         7706 a         2141 c         3519 g         6050 t
ORIGIN      
        1 tatagggtaa taaatacata ttatatttat tttactatat ttttttacat aatatatcta
       61 gaaaagtata ggaatttatc gtaatttaag tttaaatatt aaagagaatt tctataagta
      121 gtttaatcga caacttaaat ttataaagaa atttaaattt gaaagctagt gagtacagtt
      181 tggaaattga aataaagtaa actaaatgat attttagatt tggaggtgat attatgttca
      241 gggtagttat atgtgatgac gaaaaaatac aaagaagcat actaaaagag tttacaaaaa
      301 aacttttgtc tgaaagatgt ctaaattatc aaatactgga attttcctgt ggagaagatt
      361 taatatccaa atacccagaa aaaattgata ttatattttt agatatccaa atgaaagata
      421 tcaatggaat tgaggtggct aaaaaaataa gaaagtttga tgaaaaagta gaaattatat
      481 ttacaacagc tttttcagag tatgcccccc aaggttatga ggttcgtgct tatagatatt
      541 tagtaaagcc tatagagtat agtaactttg caatgggtgt aaatctttgt attgataacc
      601 ttcaaagaaa gaaggagcgt tatatagttt taaatagtaa aaaaggattt aatagaatac
      661 tgatagattc tattttatat gttgaaactg taaagagaga tttagttgta catacaatag
      721 gtagagatta taaagctaat atgagcatga aacaagcaga aaaattactg tgtgaaaatg
      781 gcttttttag atgtcataca agttttattg ttaatttaag aagaatagaa gatcttaggg
      841 ataatatgat tactattaat gaacaattta tacctataag taagtataga cttaaagatt
      901 taaaagtagc attaacaagc ttgttatgtg atataatgta ttaattttaa aaaggtaatt
      961 gaaaatatcg taaaaaagat ttaatgtcat cacagggcta ttgattaaaa ctgtcttaat
     1021 atataaaatg ttatttatat taagacagtt ttattatgca aaatatcagt ttatagactt
     1081 ttaatattgt ttaatatgta tatgtagagt ttccattaca gtagcatgat tattaattag
     1141 tagcctgatt attaatatac tattattgat ttttatatgc attatatgta tttttagaat
     1201 atgattacct ttttaaaata atgactattt ataaaactct atagatattt gtttcattta
     1261 gctatgcata ctttaattaa ttatattaat atttaatgga gtataatata aatattaaag
     1321 cgacatctta tagtttattg aacctatagt aatgaattaa taaatggttt aattctatga
     1381 aaagatttcc ttttatgcaa tttatttatt gaaatatgat ataatatata aaggtgtaag
     1441 tattagttgt taaaataata tttatattag tttttatgca tacttggtat aagtttttca
     1501 tatattggtt aaaattgatg ttgatttatt atttttagta ttttttcacc ttttgattca
     1561 tactttgtag aatggtgtat tttataaaac tgattataag acaagttcat tcaaattagc
     1621 atttttatat catataaata attgttcccc ctaaataaga tttagatgta tagtattaaa
     1681 atcaaaaagt ataaaataaa attgatgctt aatcatagat aaatattaat aaaatataca
     1741 aggggattgt ctaataaaaa gataatctcc ttttttacaa ataatcctaa attgtgatat
     1801 aaatctaaaa attttatctt aacatttaac catttaatga taataattga tataataaat
     1861 ataaattaaa tatatggtgt acttatttta tgtttttagc aagaaatata gcatttaaaa
     1921 gataaattgt gcaacttaaa tcaaagttta agatgaacta ttaacataga tatattaaaa
     1981 acttttgtaa tggagaatat atgtgatatg aataataaag ttaaggttgc agtattagat
     2041 acaggaatag acaaagaaca tgattactta aaagataatt tagttggagg aattgctttt
     2101 gaatgtatgc atgattatat ctttatatca gataaatttg atgatgaaga tggtcatgga
     2161 actgcttgtg catcaataat aaagaaagag tatgaagatg tagagttatt tgtaataaaa
     2221 attttgagaa aagatggtat tacaaatata aaagttcttg aagaagcatt aagatacctt
     2281 ttagacacta acataagatt aattaattta agtttatcag tcataggagt agagagtgtt
     2341 aagggcttat ttgaagtttg ttacgaattg tttagaaaag gaaaaataat agtatgttca
     2401 ctcgctaatg attttgattt aagttatcca gctatgttta acaatgttat aggtgtaaga
     2461 ggatttactt tagatggaga tgattcattt tggtacaaca aaaaatatga tgttcaatgt
     2521 gttatggata gcaattcata tataagttgt gatataaata attcctatag attaccacca
     2581 aaatgtaata gttatttatc agcaaagctt actggtaaaa tagctaagat attatcagaa
     2641 aaaccaggca taacaatatc tgatttaaat gataagcttg aatcattagc aactagaaat
     2701 cattgggata gttgtgattt tgatgagtgt agtagaattg taaactttaa attggatttg
     2761 tatgacaaag aaaatgctct tcttatgaaa gttgctgacg ttgttaggga ttgtttagat
     2821 attgaagata ataatgaaaa actctttaag ggtagccttt ttaataagga gataggtttg
     2881 gtttatgata attgttttaa tctgttgaaa aagctagagc atagatttga cataaagttt
     2941 aattatatgg acatctcaaa atatgactta gtttcaatat atacactgac tgaattagtg
     3001 gaaagatata aaaacttata aataaagatt tgtagctaaa tttaatgtag aaggtattaa
     3061 attatatgta ttctttgtcg gtagattaaa agtaaaaaat atactataat tagatagcat
     3121 aagagaaaat gccaatacag ggggagttag ataatgaaat atgaagaagc attagagtac
     3181 atatcacaaa ctaataaatt cggtataaga ttaggattag aaaatatcgg aaagttatta
     3241 gaattattag gaaatccaca agaaactttg aatataatac atgtagctgg aactaatgga
     3301 aaaggttctg tgtgttcttt tgtttctaat atactaagag agtgtggata taaagttggt
     3361 ttatatacat ctccatattt agaaactttt actgagcgta taagagtaaa tggacaaaat
     3421 ataccacagg atgatgttgc tagaataatt gagttaataa aagaaaaaat agagattatg
     3481 gtaaaagaag gctatgcata tccaacagaa tttgaagtgg ttactgcaat ggcattttat
     3541 tactattcag aacaaaaggt agactttgtg gcattagaag ttggtttagg tggcagatat
     3601 gatgctacaa atataataac aaaatcactt gtaagtgtga tagtatctat aagtttggac
     3661 catacaggta tacttggaga tacaatagaa aaaatagcgt acgaaaaggc tggaataata
     3721 aaggaaaatg gagtagtact agtatatgac cagactgatg aagcgaagga tgttataaag
     3781 tcagtttgta aagagaaaaa agctaaatat atagaagttg attttgatga tataaacata
     3841 aaaaaatcag atataaattc tcaaatatac gattgtactg taatgaaaga aacttataga
     3901 gaccttgaaa taaaattgat aggtgaacat caaataaaca attctatctt ggcaattagt
     3961 gtaataaaat atctaaaaga cataaataaa ttagcaaata taaatgaaga atctataaga
     4021 aaaggactta taaatactaa atggccagga agaatagaaa agataaaaga aaatccaatt
     4081 tttataatag atggtgcaca taatgaagat ggagcaaaat ctttggcaaa agcacttgat
     4141 aagaatttta aaggtagaaa attaacctta ttaataggta tgttagaaga taaagatatt
     4201 gatagtgttt tagagatatt gttgccacat tttaataagg taataacaac aacaccaaat
     4261 aacccaaggg ccataaattc agatatatta agagaaaaag tattaaaata tgtagatgat
     4321 gtaacatcaa agcatgagat agaggatgca gtaaattata cattagaaac atcaagtgag
     4381 gatgatatta taataagtgc aggttcatta tatatgattg gtacagtgag aactttagta
     4441 aagaaattat aattttataa aataaatata caaataaaga tataaaaaag agttgtctaa
     4501 aaaatggaca actcttttta tataaagtaa gtttttaata attttactga tgtttatata
     4561 agtaattata tagattgacg tatagcctta gataattggt ctgggcaaga agtacctttt
     4621 ccaccacagt caataccgtt taatttatca gcaacttcaa gagcattttg gccgattatt
     4681 aagcttctaa gtcctgataa attaccatta catcctccta taaactcagc atcaacaatc
     4741 acattatttt catcaacttc aaaaatcatt tctctacaac atacaccact tggattaaat
     4801 gttactttca ttaaaaagac ctccattaaa tattatttct ttttttaaca ataataataa
     4861 taccaaatat atgcgtaata atgcaactat aaagcataat acaagctaat gaaataatat
     4921 attattgtag tatatgtatt gtagagagga ggggttatat gaaaatctat atttctaact
     4981 acttatcaaa gagtattagc atagttgatt attctacact tgaactagaa aaagaaatag
     5041 tattagaaga taatatatat ccacaccatt tttgcataga aaaagaaaaa aatttaatat
     5101 acatacctag ttcaagtaat gggatacttt atgtcttaga tttaagtaat gataaaataa
     5161 ttgatactgt ctctatagga ggaagtctta gtcaagttgt acttagtgat aatgaattgt
     5221 ttgtggcaaa tgaagattct aatagtatat atgtactaga taaaaacaca ttaaatccaa
     5281 taggaattat tggagttgat aatatgccac atggatttga ctttgataga gaaagtaaaa
     5341 aattatatgt accgtgtata aactcgatag tatgtataga tacaattaat aaaaatatac
     5401 aaaagaaaat aaatatggac tttagagctt ggcatataga gcttgataaa caaaagaaag
     5461 aaatgtatat atcaacactt gatggaaagc ttataataat agatgaagta agcatggata
     5521 taaaaaaggt attatatgaa tttttattac cagtagaaat aagatttaat tatagtggca
     5581 aaaaagttta tgttgctgat ttgggttata ataatgtaag aattttagat tatacaacga
     5641 gtagatacat tggaaatatt gaaataaatg ggattcctca aggcttagaa atatctaaag
     5701 atgaaaagct actatttgta tcagatacac aagaaaattc agtaaaagtt tatgaaactg
     5761 caaacaataa attaataaaa gaaatcaagg taggaaaaga gcctacaact ataatttgtt
     5821 tgtaggcttt aatgtatgtc tcttataagt aaatcgataa cttcggcata catataagat
     5881 gcattatttt tccagttatt gcaaataagt tttgcttgtt tctctgatga aacatttaga
     5941 tttaaatcta ttaagttaga ctgattttct atgactctaa ggttgacaat aaattcatta
     6001 tcactttgtt ttacaaaact agactttact tgagtatctg ctagtagatt ttctttgtta
     6061 gaatctatat acttgtctat tttttcagta acactagtag gtattcgatt aaagaagtat
     6121 tctagacttt ctacgcctct ttgagtaaga gcataatatt ctctgtcaga gtctttatat
     6181 gtagtcaaaa acttagattc cataagctga gataaaagct gttgtaatga aaaataattc
     6241 atcatctcag tctctaatac tacttgagtt atttgagaat tagttaaatc cattttaatt
     6301 ttttctaata tatataaaat taaaagtttg tgataggcta attcttcaga cgagttttca
     6361 aacataaata tcatcctccg tcaaactatt tctatatata atatattaaa ctaatttgta
     6421 caaaattaaa agaacaattt gtattgtatt attaatcttt aataattcat ataaaacaat
     6481 tttataaata aatatagaga aacagggctg ctaattagca gccctgttga tacccaggtg
     6541 tcaaaattta ctatttattt tccagccatt tgtctttcag ccatttcaac taatttctta
     6601 gtcatatatc caccaacata tccattttct ctagctgtta agttaccttt atctatgttt
     6661 tcatagttag ataaacctat ttcgttagct atttctaatt tcatttggtt taaagcagct
     6721 tttgcttctg gaactactgt tctgtttgaa tttgacatag taaatttcct cctcaaataa
     6781 ttagtttatg atgtctaaca acacttttat ttatgtgatg ttaatactaa tttctccaaa
     6841 ataaaaataa tatatacaga aaaataaagg taaaaatgta aaaaatataa aaaaattaaa
     6901 taaatgagta ttttagagag tttaattagg tcaatatagg ttttattttt ttagttattg
     6961 tcttataaat taaatattac agtacttttt tgagctgaaa aaaaataaat tttaaatata
     7021 gaagaaatta tttcaaaggg ggaatagtat gaaaagagaa gttaaaagga ctaaagcttt
     7081 gatatatagt gttttatttt tttcattatt attagtgtta atatttgttt ttgtttatgg
     7141 aataaaatac atatgttcta aatacgacaa ttcaaaaatg cctatatata aagttgatac
     7201 aaatgagaaa aaaatagcct tgagttttga tgtagcatgg ggaactgata atatggatga
     7261 tattttaaaa attttagata aacataatgt aaaagctacc ttctttttag ttggtagttg
     7321 ggtggatgat aatgaagaaa ttgtaaaagc tatagatgaa aaaggacatg aaattggaaa
     7381 tcattcaaac actcatgcca atttgaagga aatatcaaaa gaagacataa caaaagagat
     7441 agaaactaca tcagaaaaaa tatataatat aacaggaaaa aagactaatt tatttagacc
     7501 tccttttggg gacgtaaaca ataaagctat ggacatttgt agtgatttag gttataaagt
     7561 aattaagtgg gatgtagatt ctatagattg gaaggagctt ggaccaaatc atgtaattga
     7621 gaaagtaata aaagaatctc aacctggttc aatagtttta ttccatgcta atataaatga
     7681 tgtagataac tatttggata ctataataag tcgactaaaa aaagatggat acagtctagt
     7741 caaaatatct gatttgttgt ataaagataa ttatgtagtt gattccaatg gagtgcaaaa
     7801 aactaagaat taataagtaa taaatgtata ttttaagtaa agttgtgata aattatatag
     7861 tatatgttag aaaagtgtcg ataaaaagac ggtgtctatt ttttgacact ttttatacat
     7921 gtatgcaata tcaataatta tagattaaat tgattaaaaa agtgtctaat aattgacttt
     7981 tttttgatgg atttaaaaat ttagaagaat taatgaatgc tgtaagacag tatttccaaa
     8041 gggtataagt ttgtttaaaa gttggcatag aaattgcttt gttaataagc gatatgttct
     8101 atagaaataa acaatatgtt aagaccttat aatagaaaaa tagcattaac attatttgaa
     8161 cagcttataa aaaaattaat atacttaagg gggcgtacta gttgaacaaa ctatattcta
     8221 ttttattaaa acaacacgtt ggtggtccag ataagccagt agtaagtgtt ggagatgtag
     8281 taaaaaaagg aactctaatc gcagaaccaa ctggattagg ggcaaatatt tatgccagtg
     8341 ttagtggaaa aataagtgaa ataaatgacc aagcgatagt aattgaagct gatgaagtac
     8401 aagaagatac ttttgaacca ttaaaaggag aaggtatact tgacttaatt aaagaagctg
     8461 gagtagtagg aatgggtgga gcaggattcc ctactcatgt aaaattaaac atagatttaa
     8521 atggtggaac aatattagca aatgcagctg aatgtgaacc tttattagct cataatataa
     8581 aagaaataga agaaagacca gaaatagttt atcaaggtat aaaatatgct atggaagtta
     8641 ctaatgctgg aaaaggtatg ctagcaataa aaagcaagca tccaaaagca atagaagctt
     8701 tcaaaaaagt aataaagcca ggagacaaca tagaagttgc agaactagtt gatatgtacc
     8761 caatgggaga agagagagca atagtaagag acgttctagg aaaattactt gagccaactc
     8821 aattaccttc agaggctaat gcagtagtaa taaacgtaga aacactaact agaatagtag
     8881 aggctgttga gcaaaaaaga ccagttatat ctaaaaatat aactgtagtt ggtcaattaa
     8941 acagtggaaa agagtctata gtatttgaag atgtgccaat tggaacaact gtcggagaat
     9001 taattgaaag agctggtgga ataaaaggtg aatatggtga aataatccta ggaggacctt
     9061 tcactggtaa agctactact ttagatgctc caataactaa aactagtggt ggtataatag
     9121 ttactatgcc atttgttaat gaaaagagaa aaatgggtct tttagtctgt gcatgtggac
     9181 ctaatgaaga gagaatgaga gatatagcaa ctaaaatggg agttactgat atagtatcag
     9241 ttcaaaaatg taaacaagct caagaaatta aaggtgcatt aaaatgtgaa aatccaggac
     9301 actgtccagg acaagctcaa aaatgtattg aatttaagaa agctggagca gaagttatat
     9361 taataggaaa ctgtactgac tgtagtaaca cagtaatggg ttctgcacca aaattaaagt
     9421 taggtgtata tcatattact gaccatgtaa tgagaacagt taatcaccca ttaataagaa
     9481 gaataaaagg tgacgtaaga ttaaaaataa aataatagat aaatagaagt tagacatctt
     9541 tagaagtaaa acttaaaaat tacttctaag gatgtttttt aatgtatttt gagggcaata
     9601 tatagcttta aaagaaatca aaaaagtgcc aaatgtaaaa ctaattaaac catatatatt
     9661 taagaaggga ttgaattgga gttgtgaatt atgttagtag aaaccattct aagtgtttgt
     9721 tattggtaca tttgatattt gtatgtaaat acagaaaaaa ctttttgctc agatggatac
     9781 tttgctttca gtgtaggaaa tgtttctaaa gaaatgattg aaaaatacat acaaagtaag
     9841 ggataggttt acatccacca acctaaaggt atagtggttt tacgccttaa tttataaaaa
     9901 gaaattaatt tatattatta ttaagtttaa aataaaaagt ctcaaattga tttataagtc
     9961 attttgggac ttttttatgt aaatataata tgcaaatata gtttgtttaa aaatatctat
    10021 tttaattata ttcttaaatt tatgattgga tacaggcatt cattaattgt ttgatgaata
    10081 gaaaaatgtt ttgaaggtga aaatagtatt gaaaaaataa tatactaagg gtataattca
    10141 tttgatttca tttgtataaa tttaattata aattttaaag tgattatata aatttaaatg
    10201 ctaattactt aataaatttt aataataatt atttaataaa taattattat aatatgctag
    10261 aataaatata tataaataag ttttgatgag gtactactat aaaggggggg actttatgtt
    10321 ttcgatacca gaagtaaaaa aagtagaaga ggtaatggat acaaaattta caactataga
    10381 cgaagacaca agaattgaag atgcaataaa agaaatgata aaaagcaaca caaaaactct
    10441 aatggtaata gattcatctg accaattaaa aggcattata tccatgacgg atatacataa
    10501 tctttatgag atgcataaaa agtatgaagg tcaaccagta aaacttataa tgaagaagga
    10561 tgtaatatat gtcaacgaag gtttaacctt agatgaatgt agagatataa tgatattaaa
    10621 aaatataggg attcttcctg ttcttagaga taataaaatt attggagttc taaaacaaga
    10681 gcacattagg gactatctat atatgcattt agaagattat ggattgactt tgaagtatat
    10741 tataggtcaa attaaagaag gaatttgtgc tataaataac gaaggtgttg ttatcttgtg
    10801 gaataagttt atggaggaaa ggtatgatat aaagtctgaa gatatagttg gtcgaccgat
    10861 gaatgaattt ttggaaaata ctatttcaga aaaagtacta aatagtaaag ttggaatgag
    10921 tgacttgtat tttacagata aaaaagaaaa tatgtatgca ttagttcatg caaatcctat
    10981 attttataaa gaagaattta tcggtgttgt atgtacagaa gttgatgtta cagaagctaa
    11041 gatattagct cttgaacttg aaaaagtaaa tgatacatta aaatatctaa agaacgaagt
    11101 caaaaattta tctaaaggta gttttgataa gattttaggt aaaagttata aattggaaaa
    11161 atcgaaagcg attgcaaaac aagtggctag aacaaatagt agcatattta tttggggtga
    11221 aagtggtacg ggtaaagaag tatttgcaag agctattcat gattatagtg agagaaaagg
    11281 tcaatttata cctgttaact gtagtgccat accaaatgag ttatttgaaa gtgagttttt
    11341 tgggtatgaa tcaggtgcat ttacaggagc aagtaaaaag ggtcgaatag gtatatttga
    11401 acttgcaaaa gatggaactg tatttttaga tgaaattgcc gatttacctc ttagtatgca
    11461 agcaaagttg cttagagtat tacaggaaaa agaaataaga agagttggtg gagataccac
    11521 aataaaaata aatcctagaa taatatcagc taccaataaa gatttgagta aaatggtaaa
    11581 ggcagaaaaa tttagagaag atttatacta tagattaaat gttgttgaaa taaagattcc
    11641 tccacttaga gagagaaaag aagacatagg attattggtt catagctttt tagaggagat
    11701 atgtaaacaa aataataagc ctgttcttac aattagcaaa gatgtaatag atatatttca
    11761 gaattataga tggaaaggta atattagaga attaaaaaat acgattgaaa atattgttgt
    11821 attgtcacaa aattctaaaa tagatgtaga tgatgtacca agttatatga tggattctac
    11881 aaataatagt actgaagaag aagagtatcc cttagattta acgaaggcaa ctcaaaaaat
    11941 tgaaataaag aatataacca aggcacttaa aatgtctaat ggaaataaag ctaaagcagc
    12001 taagatatta aatataccca gaacaacttt gtattataag atagaccaat acaaaataga
    12061 tgtgtctaaa atatgacata tgactaaatt ttgacaaaat gataaacttt aatttaaaac
    12121 ctagtaaagc caacacttgt ataagtgttg tgtatataga tgtgttaaaa aaaatgaaaa
    12181 agtgttgatt aattgacact ttttttgttt gagaaaatgt ttacaaatac aaaaaataac
    12241 atattttaaa taaatattta tataatattt ggctaaaaat agcgatagat gaagaatcta
    12301 ttatagaaaa aatctttttg ttaataagtt ggcataggaa ttgcttataa tataaagtga
    12361 ctttgagtga gaaaaaaata atattaatca taacaaatta tgtaggtttt aatttactta
    12421 atttctcagt taaggggtta cctgttgagg aattgagatt taaaataata aataagaaaa
    12481 taatgtaagg aggtatttta tactatgtca ataactttag aaacagctca agcccatgca
    12541 aatgacccag cagtttgttg ttgtagattt gaagcgggaa caattatagc gccagaaaac
    12601 ttagaagatc cagcaatatt tgcagactta gaggattctg gattattaac aataccagaa
    12661 aatggattaa ctataggtca agtactagga gctaagttaa aagaaacttt agatgcactt
    12721 tctccaatga ctacagataa cgtagaagga tacaaagcag gagaggctaa agaagaagta
    12781 gtagaagaaa cagtagaaga agcagctcca gtatcagaag cagcagtagt tccagtaagc
    12841 acaggagttg caggtgaaac agttaaaata cacataggtg aaggtaagaa cataagctta
    12901 gagatacctt tatcagtagc tggtcaagca ggagttgctg ctccagtagc aaacgttgct
    12961 gctccagtgg caagtgcagc agcagaagta gctccaaaag ttgaagaaaa gaaactttta
    13021 agaagcttaa ctaaaaaaca ctttaaaata gataaagttg aatttgctga tgaaactaaa
    13081 atagaaggaa ctactttata catcagaaac gcagaagaaa tatgtaaaga agctaatgaa
    13141 actcaagagt tagttgtaga tatgaagtta gaaataataa ctcctgataa atatgaaact
    13201 tacagtgaag ctgtattaga tatacaacca atcgctacta aagaagaagg cgaattaggt
    13261 tcaggtataa ctagagttat agatggagct gtaatggtat taactggtac agatgaagat
    13321 ggagttcaaa taggtgaatt tggttcttca gaaggtgagt taaatactac tataatgtgg
    13381 ggtagaccag gtgctgctga caaaggtgaa atattcatca aaggtcaagt aacaataaaa
    13441 gcaggaacta acatggaaag accaggacct ttagctgctc accgtgcatt tgactatgta
    13501 actcaagaaa taagagaagc attaaagaaa gttgacaact ctttagtagt tgatgaagaa
    13561 gtaatagagc aatacagaag agaaggtaaa aagaaagttg ttgttataaa agaaataatg
    13621 ggacaaggtg caatgcatga taacctaata ttaccagttg agccagttgg tacattagga
    13681 gctcaaccaa acgttgactt aggaaacatg ccagttgtat tatctccact tgaagtatta
    13741 gatggtggta tccatgcatt aacttgtata ggacctgcat caaaagaaat gtcaagacat
    13801 tactggagag agccattagt aataagagct atggaagacg aagaaataga tttagtaggt
    13861 gttgtatttg ttggttctcc acaagtaaat gctgagaaat tctatgtatc taagagatta
    13921 ggtatgttag ttgaagctat ggaagttgat ggagctgtag taactactga aggtttcgga
    13981 aacaaccata tagatttcgc atctcacata gagcaaatag gtatgagagg tataccagta
    14041 gttggtgtaa gtttctcagc tgttcaaggt gctctagttg ttggtaataa atacatgact
    14101 cacatggtag acaacaataa gtctaagcaa ggtatagaga atgaaatatt atctaacaac
    14161 actttagctc cagaagatgc tgttagaata atggctatgc ttaaaaatgc tatagaaggt
    14221 gtagaagtta aagctcctga aagaaaatgg aatccaaatg ttaaattaaa taacatagaa
    14281 gctatagaaa aagttacagg agaaaaaata gtattagaag agaatgagca atctctacca
    14341 atgagtaaga agagaagaga aatatacgaa aaagacgaaa actaaaattt agatactaac
    14401 taagatttag acaataacat aaatttagat ccaagtttaa aacagtatag agaggtagaa
    14461 ttataatgga taggttaaaa tatatctcat cagagacttt ttatgaaggt attatagttg
    14521 acatcaaagg tggtggagtg actatagacc taaaaggtag acttggacaa tttaaaatac
    14581 ctaatagaat gcttataact gactacgaac ttaagatagg tcaagaagtt ggattcatgc
    14641 taagctatcc tgaggtatta agtccagagc ctaatcaaga gtatgtagaa aatattagga
    14701 gagaaaaaga aaaacaagca gaaatgcaat caaaacaaga agaaaaataa taaataatcg
    14761 tttgaaaata gaagggagag gaaatatatg agccttacaa cagtacaagg acttcaatct
    14821 gaaatattcg ttccaataac acctcctcca gtttggactc ctgtaactaa agaattaaaa
    14881 gatatgacta tagctttagc tacagcagct ggtgttcatt taaaatctga caagagattt
    14941 aatctagcag gagatactac atttagagct atacctaata cagctacagt tgatgaaatg
    15001 atggtatcac atggtggata tgacaatgga gatgtaaaca aagatataaa ctgtatgttc
    15061 cctatagata gattacatga attagctgca gaaggattta tcaaaggagt agctcctatg
    15121 cactatgcat tcatgggtgg tggaggaaac caacatgtct tcactgaaga aactggtcct
    15181 gctatcgctg ctaaacttaa ggaagaggga gtagacggtg tagttatgac agctggctga
    15241 ggtacttgcc atagaactgc cgtgatcgtg cagagagcaa tagaggaagc tggaatacct
    15301 acaataataa tagcagctct tcctccagta gttagacaaa acggaactcc tagagcagtt
    15361 gctccattag ttccaatggg tgctaatgct ggtgaaccac ataatataga aatgcaaaca
    15421 catatattaa gagatacatt agagcaatta gttgcaatac catctgctgg taagatagtt
    15481 ccattaccat acgaatataa agctcacgtt taaaatgtaa tttatatttg aaaatataga
    15541 acccttctcc tacacggtta ggagaggggt tttaagaaca aaggtggata ttatggaaga
    15601 aaaaataatg agaagacttg ttataaaacc gtttcatatg aatgaagtta atttcggttc
    15661 aaaaacttct ataaagaaag atgtacttac aatagattta tcaagtatag atgaaataaa
    15721 agaaagagaa gagttaataa cagacataaa agtagacatt ataaaacctg gagattatga
    15781 tagagaaatt aatactatta tggacattat accaatatca acaaaagttt taggcagact
    15841 gggagagggt atcacacata ctttaacggg tgtatatgta atgttaactg gtgcagatga
    15901 agatggaaat caaatgcatg aatttggttc ttcagaagga attttgaaag accaaatggt
    15961 atttgggaga tatggaactc catcagttga agattatata atccatgtag atgtaacttt
    16021 aaagggaggt ttaccttttg aaagaacact tccacttgca gcattcagag catgtgatga
    16081 ttttatacaa gaaataagag cgtctttgaa aatggaagac ggaagaaatg ctactcaagt
    16141 tcgtgaatat tttgacaaaa ttcgaccaaa tgcaaaaaag gttgtcatag taaaacaaat
    16201 agcaggtcaa ggtgctatgt atgacaatca gttattctca aaggaaccta gtggatttga
    16261 aggaggaaca tcaattatag atatgggaaa tgtacctatg attatatctc ctaatgaata
    16321 cagagatggt gcccttagag ctatgactta agagaggtgg atattatggg aataggacct
    16381 tcaacaaaag aaacatctct tcatcacttt agagacccac ttttagacat agttgaaagt
    16441 gataaggatg tagatttact tggagtaata gtagtaggaa ctccagatgg aaatgaaaat
    16501 aaaacctttg tgggacaaag aacggccgct tggctggaag ccatgagagt agatggagct
    16561 atagtttcat cagatggttg gggaaactca catgttgact acgctaacac atttgaggaa
    16621 ataggaaaaa gagatatacc tgtagttgga gttacattta atgggactca agctaagttt
    16681 gttgtaagca atcaatatat ggataccata gttgatatga acaaatcaaa ggaaggtatt
    16741 gagacagaag tagttggaga aaacaacaca aatgaaatag atgctaaaaa agctttagca
    16801 tttttaaaac ttaaaatgag aaaacatgga taaaaaattt aagtaaggga atgagttttg
    16861 tgggaatttc tacatcaatg aaaaacacat cacttcatat atttagagac ccgttactag
    16921 atttgataga taatgattct gacatagact tgttaggagt ggttgtagtt gggacatcag
    16981 agcacaatga atggaaaaca tttttagcaa ctagacttgg aagatggatt gaatcattgc
    17041 gacctgatgg agtgattata actttagata tggcaggaaa ccagcacatt gattttacaa
    17101 atgctatagc agagtttgta aaatcagata tacctgcagt aggtcttact attatgggag
    17161 cagatggatt agtaattacc aatccatatt tggataaagc taccataatt gattataaaa
    17221 agacaacagg ctacatagaa acagaagtag ttggagacaa tcacatggat gaagttgatg
    17281 ttaaaaaagc attggctttc ctaaaattaa aaatgcgtaa agacgcaaaa taaacctaag
    17341 ggggaatttg atatgaaatt tagcagaagt atacaagcta tagactctca tacagcaggt
    17401 gaagcaacta gaatagttgt aggtggaata cctaacataa aaggaaattc tatgcctgag
    17461 aaaaaagaat atttagagga aaatctagat tatttaagaa ctgctataat gttagagcca
    17521 agaggacata atgatatgtt tggttctgta atgactcaac cttgttgccc agatgctgac
    17581 tttggaataa tcttcatgga tggtggtgga taccttaaca tgtgtggcca tggtacaata
    17641 ggagctatga cagcagctat agaaacaggt gtagttccag cagtagagcc tgtaactcat
    17701 gtagttatgg aagctccagc aggaataata agaggagacg ttacagttgt agatggaaaa
    17761 gctaaagaag tatcattctt aaacgtacca gctttcttat ataaagaagg tgttgaagtt
    17821 gacttaccag gtgttgggac tgttaaattt gacatatcat ttggtggaag cttctttgct
    17881 ataatacatg caagtcaatt aggtttaaaa atcgaacctc aaaatgctgg taaattaact
    17941 gaactagcta tgaaacttag agatataata aatgagaaaa tagaaataca acatccaact
    18001 ttagctcata taaaaactgt agacttagtt gaaatatatg atgagccaac tcatccagaa
    18061 gctacttaca aaaatgtagt tatatttggt caaggtcaag ttgacagatc tccatgtgga
    18121 actggaacaa gtgctaaatt agctacttta catgctaaag gtgaattaaa agtaggagaa
    18181 aaattcgtat atgaaagtat attaggaact ttattcaaag gtgaaatagt tgaagaaact
    18241 aaagttgcag atttcaatgc tgtagtacct aaaataactg gttctgctta tataactgga
    18301 tttaatcatt ttgtaataga tgaagaagac ccacttaaac atggatttat tcttaaataa
    18361 gtaataaata aattaggaaa ttaaaataga atagtagcca cttgtcggtt gctattctat
    18421 agcttttagg ctataagatt cagaatatat gaaaaaataa aaaaatagga ggatggatta
    18481 tggtagcaat cttaaaggca ttatatgtag tattcgcagc aatctttgca gttgtgtttg
    18541 gtaaagacgt agctaaagca aacaaagagg ggaaactaga agaacaaagt actccaaaag
    18601 ttgctgtaac aggatttata acagacttct ttgatacatt aggaataggg tcatttgctc
    18661 caacaatagc aatgtcaaag gctttaaagt taaatatacc agataaaaaa atgcctggta
    18721 cattaaacgt agcacatact attccagttg ttacagaagc attcatattt acatcaatca
    18781 taccagttga tggtgttact ttagtatcta tggttgtggc agcagcagtt ggttcatata
    18841 taggtgcagg aataatagct aagatggacg agagaaaaat acaacttgtt atgggtgtaa
    18901 ctttagcatt aacagcaata ttaatgttat taggtcatcc ttggattaac gtattaccag
    18961 gtggaggaaa tgaattaggt cttactggtg ttaaattggt aataggtgta ataggtaact
    19021 ttatactagg agctttaatg acagctggta taggtctata tgctccaggt atggcaatgg
    19081 tttatttctt aggaatgtct gcaaaagttg cattcccaat agttatgggt tcttgtgcat
    19141 tattaatgcc agttgcaagt atgaaattta taaaagaaga tgcatatact aaaaaagcat
    19201 cagttataat agctatatgt ggtttaatag gagttttcgt agctgcatat atagtaaaaa
    19261 ctctaccaat ggatatatta aaagcattag taatagtagt tatagcttat acttctgcta
    19321 caatgataat ggctgcaaat aaaaacaaaa agactgctta gttaaagact acttagtgtt
    19381 tattaaatat tacagctaat aataaagatt ataaac
//
LOCUS       DAEPLF010000053        19253 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEPLF010000053 DAEPLF010000000
VERSION     DAEPLF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.053"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            290..811
                     /locus_tag="KR314_001652"
     CDS             290..811
                     /locus_tag="KR314_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0328040.1"
                     /translation="MDIAKLILNPARLRILQYIRLHGSARTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVVDNDKTK"
     gene            845..1621
                     /locus_tag="KR314_001653"
     CDS             845..1621
                     /locus_tag="KR314_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0328041.1"
                     /translation="MKFHEFGDKNKPHIMLIHGGGNAWWNYLRQAEVLSNNYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKGYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1964..2917
                     /locus_tag="KR314_001654"
     CDS             1964..2917
                     /locus_tag="KR314_001654"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBH0328042.1"
                     /translation="MINVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2935..3954
                     /locus_tag="KR314_001655"
     CDS             2935..3954
                     /locus_tag="KR314_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBH0328043.1"
                     /translation="MFKKCIKVVTLTFILACILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEENTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3985..4236
                     /locus_tag="KR314_001656"
     CDS             3985..4236
                     /locus_tag="KR314_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328044.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4518..6323
                     /gene="lepA"
                     /locus_tag="KR314_001657"
     CDS             4518..6323
                     /gene="lepA"
                     /locus_tag="KR314_001657"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBH0328045.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6474..7811
                     /locus_tag="KR314_001658"
     CDS             6474..7811
                     /locus_tag="KR314_001658"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBH0328046.1"
                     /translation="MINIEIPKKVDYIIKELETNGYEAYVVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLISTEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILRRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            8044..9357
                     /locus_tag="KR314_001659"
     CDS             8044..9357
                     /locus_tag="KR314_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0328047.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTIISLGGIFITLLGLPVYYYMKKR"
     gene            9529..10704
                     /locus_tag="KR314_001660"
     CDS             9529..10704
                     /locus_tag="KR314_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBH0328048.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMKLY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINVDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10807..11826
                     /gene="hrcA"
                     /locus_tag="KR314_001661"
     CDS             10807..11826
                     /gene="hrcA"
                     /locus_tag="KR314_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBH0328049.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11856..12476
                     /gene="grpE"
                     /locus_tag="KR314_001662"
     CDS             11856..12476
                     /gene="grpE"
                     /locus_tag="KR314_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBH0328050.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12568..14415
                     /gene="dnaK"
                     /locus_tag="KR314_001663"
     CDS             12568..14415
                     /gene="dnaK"
                     /locus_tag="KR314_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBH0328051.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLADEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14626..15780
                     /gene="dnaJ"
                     /locus_tag="KR314_001664"
     CDS             14626..15780
                     /gene="dnaJ"
                     /locus_tag="KR314_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBH0328052.1"
                     /translation="MSTKRDYYEVLGISKDAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16746..17681
                     /locus_tag="KR314_001665"
     CDS             16746..17681
                     /locus_tag="KR314_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBH0328053.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     AEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKDYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17717..19090
                     /locus_tag="KR314_001666"
     CDS             17717..19090
                     /locus_tag="KR314_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0328054.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEGNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
BASE COUNT         7634 a         2119 c         3515 g         5985 t
ORIGIN      
        1 ttactcattt ttgtaaattt aatgaaaaat tataaaaata taaatttata tgcttaatta
       61 agataatttt tccaataatc aagataattt ttttcattag tatgtatgat agatgcatca
      121 aacaatataa ctaatatgtt agaaggttat atgtaattaa tctcagtgtt tatatagaat
      181 agcatttgta gcatttatta tagttgtaat tactacttat atcatagact tgaatagtct
      241 aaaaaaatag actataatat ttttatagac taaagaaagg gctgataaga tggacatagc
      301 aaaactaatt ttaaatcctg caaggcttcg tatcttacaa tatattcgac ttcatggcag
      361 tgctcgcaca tctgatattg taaagtattt aaatgatatt cctcgtgcaa cagtttatca
      421 ccatgtaaaa attttagaag agaacaatat gattgaagta attaaagaaa atcgtgtgcg
      481 tggtactatt gaaaaggttt atgcactaaa ggaatataca acatctatgg aaggagagac
      541 cttcgttgca ctttctacag cattttatgt aggtctgatg caggaaatga atgaatactt
      601 tagcagaaaa gagcaagacc ataaaaagga taatgtattt ttctcttcag cattactata
      661 tatgagtgat aatgaatatg aaaatcttct taaaagtata gctgatttat tgaaaccata
      721 tatagaacaa aagcctaaat caaatctaaa acttagaaaa ctttcaatta tatcttctcc
      781 acctgtggtt gataatgata aaacaaaata acatgaatct tatttttata aaaggaggtt
      841 gtatatgaaa tttcatgaat ttggagataa aaacaaacca catattatgt tgattcatgg
      901 gggagggaat gcgtggtgga actatttgcg acaagcagaa gttttgtcta acaactatca
      961 tgtgatactt cctacattgg atggtcatgg agaagaatat gaaacaacat acatttctac
     1021 agaggataca gcagataagt tgatatcata tatagatgaa aattgcagtg gacatctttt
     1081 cgctttgtgt ggggtatcac ttggtggtca aattgtcatg gaacttttat ctcgaaaacc
     1141 taatattact aaaaaagcaa ttattgatgg tagtatttgc tacccaagac ctcttatggc
     1201 aaggttttgt attgctggta ttcgattttt tggaggactt ttgtttagtg agaaggcttg
     1261 tcgctttcaa attgctatga tgccaaaatt gttacctgaa aatatgcaat atccagatga
     1321 aataaaagcg tattatatga cagatatgcc acatgtaaga aaagaaacat tctataatat
     1381 gtatcgtact tatatgatga actatacact taaagagaat gttaaaaaaa ccacagcaca
     1441 agtaatgtac tggtatggag aaaaagaaat gaagtgcgtt aaaaaatctg caaaaatgtt
     1501 tcagtcttat gtaccctctt gtgagattta tgaagctaag ggctataatc atgggtattt
     1561 atcgctttat cttccaaatg aatggttgga aattgcagaa ccatttttcc aaaaggaata
     1621 aaggtgttag gtgattattg tataattatt gctgtttttt tatatagtaa ataaattagg
     1681 tataaatata aaaaagttat tttagttaat atattagtga aagcttaata ggaaaattat
     1741 aaatagatta aaggtagcgc ctatataaat agttgctacc tttttttgct tgtaaataaa
     1801 tgatagataa gaaagtttac tgacaaatca ctgactaaac ccaattttct tacattgaaa
     1861 gccttaattt tacttgtttt aaataaattt acaatttctt gttataaaat actaaatttt
     1921 gttaacaatt ataattagca tttaatttat actggaggtt tatatgatta atgtaagaac
     1981 agatttagct ttagaagcta gtgaaatgtg tgaaaagtct caagaaggaa gtagtattcc
     2041 tggtgtaaaa atagaaacta aagaattaga aaattgcatt gtaactaaag ttgaagtaat
     2101 agacaggcaa ggcgcagaga taatgaataa agatatagga aagtatataa ctctggaaag
     2161 taatcttatg aagtttgatg atgatgagtc gagagaagaa atgataagtt atttaaaaga
     2221 ggagttagta gatatatttg gccaagacaa aaataaaaaa accctggtaa ttggtcttgg
     2281 aaatagaaat attacttctg atgcgttagg accaaagagt gtatcaaaaa cacttgtaac
     2341 tagacatctt tttaaaaatt ataataaaga ctatgatgat gatttcactg aggtatcagc
     2401 tttaagccca ggagttatgg gtgttactgg aatagaaact agtgaaatag taaagtcatt
     2461 ggttgaaaaa gtaaaaccag atagagtagt agcaatagac gcattggctt caagaaaaat
     2521 ggaaagagta aactctacta tacagatatc aacagcagga atatcacctg gtggaggtgt
     2581 tggcaatact agaaaatctc taactaaaga aactttaggc gtggatgtta ttgcaatagg
     2641 cgttccaacg gtggttgatg cagcaactct tacaatagat gtcttagata tggcaataga
     2701 caatttaata gctcaatcag aagaaacaga aagtttttat gaaatgttga aaaaacttaa
     2761 agaagaagaa aaatatcatc taataaaaga ttcgttagac ccatatgaca agaacttaat
     2821 agtaacacca aaggatatag atgatacaat cgaaaatcta tctataataa taagtgaagg
     2881 cttaaatagg tctttacatc caggtagatt ggtataaaat aaggaggagc aattatgttt
     2941 aaaaaatgca ttaaagtagt aaccttgact tttatattag catgcatttt accaggaaaa
     3001 tcattagctt tgaatcagga tgatttttta aagtttttag taaattcatc ttatccagaa
     3061 gctaaagtcg aaggaaatga tacagaaaat aaaaagaata ataaaaataa agaaacaagc
     3121 aaagaaaata aggaagaaag taaagaagaa aacactaaat ctaaagatgc aagtaaagtt
     3181 gataataaaa aagagagtga aaaagaatat ataaaacttt atgttggtaa agaaaatgtt
     3241 ccagatattg agtcaaaaaa ttcagacaca actgaaacta acacaacatc tagttcagat
     3301 tataaggatg atttaagagt aaccaaagaa aatccaagaa ttttgatata tcatactcat
     3361 ggatgtgaga cttattcaaa ttcacctgat ggaaattatc attcaagaga taagaaaaat
     3421 tcagtaatgg aagtaggaag tgcattaact agtgctttag atagcaaagg atggggtgta
     3481 gtccatacta ctaaatacca tgactatcca tcttataata actcttatgc aagtagctta
     3541 aaaacaatac aaagtatatt acctaaatat aactcagtgg atatagcgat agacttacat
     3601 agagatgcta gggatttaac taatccagcc actaaggaaa aagaccattt aaaatataca
     3661 actatgataa atggagagag agtatcaaag ttcttctttg ttgtaggagg taaaaataca
     3721 aatagaaaac aacttagagc tttagcagaa gatataactg cttttgctga gaaaaagtat
     3781 cctggtctag tatctccaat agtagaaaaa gactatgcta gattcaatca gtttgcagtt
     3841 aagaatcata tgttagtaga aatagggaat aacgcaacaa gtgtagaaga atctaaggct
     3901 actacaaagt atttagccga gattttagat gaatatttta aacaaaaaaa ttagagcttt
     3961 aagttataat taggagcatg ctaaatgagc cgattagaaa aaaatataga aagtaaaagt
     4021 aaactaagaa aatacaagta tattatgaga atcttatttt tgattactat gattataggt
     4081 acatcagttt gtgtatttat agtagacagt aatgcaaaaa gtatgctagg aatgaaacaa
     4141 tatgattttc caagtattaa atttgatata acaaaatttg attttgacat accaaatttt
     4201 gatatacatg aaatcataaa aaaatttaat aagtagtaat aagtgaataa ggtttattga
     4261 ttaaaataag ccttatttta gttatatatt tttaatgtat aaaataaaaa ttcattaact
     4321 gtattttata ggatttactt taagtttacg atattatata atatttagtg ttagacttga
     4381 taatccacat ataatgtgga taattttgtt tagtgccaat tagtttatat tcaaaaattt
     4441 taaatttggt atataaagag tttttagata atgtaaaata ataactataa gaataatcaa
     4501 gaggagggaa ttttaaggtg gacaataaac aaagtagaac tagaaatttt agtataattg
     4561 cacatataga ccatggaaag tctaccttag ctgatagatt aatacaacaa actgggcttg
     4621 ttagtgaaag agacatgaaa agtcaattac ttgacaatat ggaccttgaa agagaaagag
     4681 gaatcactat aaagcttcaa aacataagat taatgtataa agcgaaggat ggaaatgaat
     4741 attatttaaa tcttatagat acacctggac atgtagactt taactatgag gtatcaagaa
     4801 gtttagctgc atgtgaagga gcactattag tagttgatgc agctcaaggt gttgaggcac
     4861 aaactttagc caatgtttat cttgctatag accaagattt agaaatatta ccaataataa
     4921 ataaaataga tttaccaagt gcaagaccag aggaagtaaa aaatgaaata gaagatttaa
     4981 tagggcttga ttcaagtgaa gctcctctta tttcagctaa aactggttta aacatagaag
     5041 atgttttaga agatattgta aaaaatgttc ctccacctaa gggagataat gaagctccat
     5101 taaaggcatt aatattcgat tcttactatg atgcttacaa aggtgttgta gcttatgtta
     5161 gagtatttga aggaactgtt aaaaagggta tgacaataaa aatgatgaat accaataaaa
     5221 agtttgaggt aactgaagtt ggtgttatgg ctccaggtca aactgaactt agtgaattat
     5281 cagcaggtga tgttggttat attgctgcta gtataaaaga tataagaagt tgtcgtgttg
     5341 gtgatacaat aacagattca aataatccta cagaagaacc tttaccagga tacaaaaaag
     5401 ctactccaat ggtatattgt ggtatatatc caggagaagg agaaaagtat gagaatgtta
     5461 gagatgctct tgaaaagctt caagtaaatg atgctgcact tgaatatgaa gctgagactt
     5521 cagcagcact aggatttggt tttagatgtg gtttcttagg tcttttacat atggagataa
     5581 tgcaagaaag acttgagaga gaatttaacc ttgatataat tactacagct ccatctgtta
     5641 tatatagagt tacaaaaatg gatggagagg ttgtaatgat acaaaatcct gcaaacttac
     5701 cagaaccatc tgagataaag atgatagaag agcctatagt aaaaggagat ataatagttc
     5761 ctaaagacta tgtgggtgtt gttatggagc tttgccagga aagacgtgga aatatgttaa
     5821 atatggaata tatagatgag agaagagtaa tgcttcacta tgaccttcct ctcaatgaag
     5881 ttgtttacga tttctttgat gctttaaaat cgagaactag aggttatggt tctcttgatt
     5941 atgaggttaa aggttatgta gcatctacac ttgtaaaact tgatatatta atcaataaag
     6001 aacaagtgga tgcacttagt tttattgtcc atgagacacg tgctttccct agaggaaagg
     6061 caatgtgtga gaaattaaaa ggagaaattc caagacatca gtttgctata cctatacaag
     6121 ctgctgttgg taataaggtt attgcaagag aaacaataag cgctctaaga aaagatgttc
     6181 ttgctaaatg ttatggtggg gatatatctc gtaagaagaa acttcttgaa aagcaaaaag
     6241 aaggtaagaa gcgtatgaga caaattggtt ctgttgaagt tcctcaaaaa gcatttatgt
     6301 ctgtacttaa actagatgaa taaattctta taaatttaga taaatatatt taaaagctag
     6361 agtgatttta gaattatttc taaattaatc tagctttttt gtggtatatt ttaagtaaac
     6421 tcatagagaa tataagtaag aatatattaa agaagaataa aaaggtgata tttatgatta
     6481 atatagaaat tcctaaaaaa gtagactata taattaaaga attagaaacg aatggttatg
     6541 aagcttatgt agttggagga tgcgttagag attgtttatt agaaagagtt ccaaacgatt
     6601 gggatataac aactagtgct agaccagaag tggtagtaga attatttgaa aaaacaattc
     6661 caactggtat acaacatggg actgtaacag ttatgattga acatgaacca tttgaagtga
     6721 caacttatag aattgatgga aattatagtg atggtagaca tccagattca atagaattta
     6781 caaataacat agttaaggac ttgtcaagaa gagattttac tatcaattca attgcataca
     6841 attcaaagac aggattagtc gacccattta atggctatga agatatacaa aataagtata
     6901 tacgttgtgt tggaaatcca gtagatagat ttgaagaaga tgcacttaga atgcttaggg
     6961 cagttagatt ttcagcacaa cttaatttta aaatagctga aggtactaag caaagtattc
     7021 ataaaaaagc agatttgata aaaaatgttt ctatcgaaag aatccagaca gagtttaata
     7081 aaatattagt atctgattca tctaaattaa acttattaaa atcaacaggt ttgttgaaat
     7141 ttataattcc tgaaatatgt gaattagaag atgttactca acacaatcca tatcacatct
     7201 atgatgtaca aaaacatact ttaatatcta cagaagtaat tgaagatgaa ctgtatttaa
     7261 aacttacaat gttgtttcat gatttaggta aaaaagtaac taaaacaact gataaaaatg
     7321 gtgttgacca tttttatact catagtagag aatctgtaaa gattgcaaag aagattttga
     7381 gaagacttaa gtatgataat tatactatta ataaagtttt aatattaata caatatcatg
     7441 attatagaat agaacctaaa aggaagataa ttaaaaagtt attaaataaa cttgaagatg
     7501 ttgaattatt tgaagattta ataaaagtta attgggctga tacacttgcc aaaaacccaa
     7561 agtatgcaaa acaaaagata ttaaatctta ttgagtgtga aaaagaattt aaacatataa
     7621 taaatcaaaa ggagtgcttt aatcttaaag atttagctat taatggaaaa gatttgatat
     7681 ctataggtat aaaaccaggt aaagatatag gacatatttt aaataagatg cttgaaatag
     7741 tcataaataa tccagaatta aatgaaaaag aaatactaaa agaaaaagca ttaaatatat
     7801 atacatttta gattcaaata taaaaaattt aattacttta aaaaattata gaagaaaata
     7861 cttatttaga taaaataaat aatttttaga gaatatttta gaagttacga ttgtatgatt
     7921 tttaaacgca tagcatttac tctaggacta tagactaata taaaaaaaaa ctttgggaat
     7981 actaaaacca taaatttata aaattaatat tttatatact gtaaaacagt gaggagggac
     8041 agtatgcata ctcaactaca aaagactatt ggtctttcag cggctctttc tactgttgta
     8101 ggtatggtta taggttcggg agtttttttt aagccacaag caatatacac tacaactaat
     8161 ggagctccag gtctaggtat tatagcgtgg cttttaggtg gttttataac aataactgct
     8221 gggcttacag ctacagaaat ttcagctgct ataccaaaaa ctggtggtat gatgatttat
     8281 atcgaagaaa tatatggaga aaagttaggc tttttaactg gatggatgca aactgtatta
     8341 tttttcccag gtacatcagc agcattagga gtaatatttg ctcaacaagc atctgaactt
     8401 ttaggaatga gcccaaataa tatggcaaat gtacttccta tagcaattgg agttatacta
     8461 tttttagcgt tattaaatat aataggttca tccttaggag gaaaagttca gacagtagcc
     8521 actattggaa agatgatacc tttaatttta ataatagtct ttggatttat taaaggtcaa
     8581 agtagtgaag tattaaatcc atttgtagga gatggtgtaa atgcatcaaa tgcacttgga
     8641 caagtattaa tagctacttt gtttgcatat gatggttgga taaatgttgg agctatatca
     8701 ggagaaatga agtctccaga gaaagattta ccaagagcaa tagttggtgg tttatcatta
     8761 gttatggcag tttatattat aataaatata gcttatcttt gggtagtacc agcaagtgaa
     8821 ttagcaacag ttacttcccc agctacatta gtagcaacaa gattgtttgg aaatataggt
     8881 ggaaaagtaa taactgtggg tatattaata tcagtatttg ggacattgaa tggatattta
     8941 ttaacaggtt caaggatacc ttatacatta gctgaaatgg gaactttacc agcatctaaa
     9001 actcttttaa aagtaaattc tggaggttct cctgtaaatt caatactact tataactgtg
     9061 ttagcctgtg tatatgcatt atctggtcaa tttaatcttt tgacagactt aactatattt
     9121 tcaatatggg tattttatgt attgactttt ataggtgtta tgaggcttag aagacaaaag
     9181 ccagatttac acagaccata taaagtccct ttatatccaa taataccaat tatagctata
     9241 ctaggtggac tgtttgtaat aataaatcaa atattgactt caacaattat atctttaggt
     9301 ggaatattta taacactatt aggtttacca gtttattatt atatgaaaaa aagataacta
     9361 cttaatttaa gttagataaa tttgggtaaa ggtgaaaatt aacttttatc caaatttttt
     9421 atactgttat acaatataag cattatatat tacttgatgt gttattttgt ttgatttata
     9481 atttagtaat tgatgcttta agataattta agaaatgagg aataaaaaat gctaggactt
     9541 tatgtacata ttccattttg tgtgaaaaaa tgtaagtatt gtgattttaa ttcttataaa
     9601 atggacatag attcaaaaaa aagatatata gaagacttaa aaatagaaat gaaattatat
     9661 agtaataaat tatataaaga taataaatat aaaaataaag aatgctgtag tctaaataaa
     9721 aatgacaaaa taacgagtat atttgtaggg ggaggtacac ctagtatact aacatctgat
     9781 gaaataagag aagtatttat aagtataaaa gaaatgtttg atatagatga gaatgcagaa
     9841 ataactatag agtgtaatcc tgggacatta acattagaaa aattaaaaac tatgaaagag
     9901 attggcataa atagacttag tatagggcta caggccattc aagaaaagca tttgaatttt
     9961 ataggaagaa tacatactta tgaagaattt gagaaaaact ataaagatgc attaagtgta
    10021 ggttttaaaa atattaatgt agatttaatg tacagtcttc caaatcaaac tctatgtgac
    10081 tggaaggaaa ctcttgaaaa agttgtacac ttaaatccaa cacatatatc agcgtattcc
    10141 cttatactag aagaaggaac tgaactatat aatatgtatg aaagtaataa atttgagctt
    10201 atagatgaaa atgtagatat agaaatgtat gagtatacaa taaactactt aaagtcaaaa
    10261 ggatataatc aatatgaaat atcaaattac tcaaaagaag gctataattg tgagcataat
    10321 attttatatt gggaatgtga acattatata gggattggag caggagcttc tggctatatt
    10381 aatgagaaca gatacaataa tgtagaatct ttagaagatt atcatttaag tttggtaaaa
    10441 agagaaaaac caatacaaga aaatgaaatt ctttctgaga aagatatgat agaagaaaag
    10501 atatttatgg ggcttagaat gaataaaggc ataaaatttg aagattttaa aaaaaaattt
    10561 ggaatagatt ttagagagaa gtataataag caaatagaaa tgctattagc tagaaagctt
    10621 ataaatcaga gctttgaagg tatacaatta actcaaaaag gaagagaaat atctaatagt
    10681 gtatttatag aatttatgga ataattttta aggtgttgac aaaataaatt tataatggta
    10741 tataatatat ataatctttt tagcactcga acttagtgag tgctaacagc taggaggtat
    10801 aataatatgg aattaaatga aagaaaatta aatatcctca aagctatagt taaagattat
    10861 atagaaacag ctgaagctat aggttctaga acaatatcta aaagacatga tttaggtgtt
    10921 agtgctgcta ccataagaaa tgaaatggct gaccttgaag aattagggta tttaattcag
    10981 cctcatactt ctgcgggtag agttccatct gaaaaaggct ataagttata tgtaaactct
    11041 cttatgagta aaagcgaatt ggatgataat gataagattc ttattgaaca atgtatgaat
    11101 cataatataa atcatataaa agaattgatt catgaaacct ctaagctatt atcgcaacta
    11161 acaaactata caactgtagc agttactaag agtttaataa atcaaagtgt tataaagcac
    11221 atacagttgg ttgctatgaa tgacaataat attttactta tagttgtaac agataaaggg
    11281 gacttaaaaa aagctaacct tacaactaat gtatatttag atcaatctaa gttaaatttg
    11341 atttctgata atcttacaag gaagctttta ggtaaaagta taacagattt ggatgataat
    11401 ctaattgctt ttattaaata cgaaataagt gagtattctg gacttataga tgagctttta
    11461 aatgctttga actcaaatat gaaagaagaa gatttttctc tatcattaaa tggagctact
    11521 aacatattta gttatccaga atttaatgat gtattaaaag caaagtcatt tttaaatatg
    11581 ttggaaaaaa aggaaacaat agctgacata attaaatcaa agggaataca aaaagacaat
    11641 ctaaatataa taattggtag tgataatgat tgtgaactgg cacaggattg tagtatagtt
    11701 acagcaactt ataatgtaga tagagacttg gttgggcgaa taagctttat aggccctacc
    11761 agaatggatt atgctagaat ttactctata ataaattata tgagtttatt aattaataga
    11821 aagtaaagaa attttaaatt gcgaggtgta aagtcttgga taaaaaaaat gagcaacagg
    11881 aagtaagaga agaaaatgat acttctatta accaagaatc ggaaactcaa gtagagttag
    11941 aagaggaagt tgttaatgaa gaatgtgaaa catcaagtga aaaaactgat gaaaaagaag
    12001 ttgatgatga aaatgtaaca gatataaatt ctaagttagc agaaaaaaaa ttgcaggatg
    12061 agttggatga attaaatgat aaatatcaaa gacttcaagc cgaatatgca aactacagaa
    12121 gaagaactca acaagagaaa gagacaatag gcgtatttgc aaatgaaaaa ataataactg
    12181 agttgatacc agttatagat agtatggaaa gagccttaga tgcatgtgaa gataaggaag
    12241 atactatgta taagggtata agccttgtac ataaacaatt aatagacact ttggtaaaat
    12301 ttggagtaga agaaatagaa gcagaatcta aggaatttga ccctaatcta catctagcag
    12361 ttatgcaaga aagtgtagat ggagttgaag caaatcaaat agtaatggta cttcaaaaag
    12421 gatataagct aggaactaaa gttgtaagac cttcaatggt taaagtttct tgctaagtat
    12481 aaattaaagg ttaaattatt taacctaata taaaatatta aaataagtaa aacttaatta
    12541 tataattact cgaggaggat tttagtcatg ggaaaaataa taggaataga tttaggaaca
    12601 acaaattcat gtgttgcagt gttagaaggt ggagaagcac aaataatagc aaatagtgaa
    12661 ggtatgagaa ctactccatc agtagtagca tttacaaaag atggtgagag aatagttgga
    12721 gaacctgcaa aaagacaagc agttacaaat gcagataaaa caataacttc tataaaaact
    12781 catatgggaa ctgattataa agtaaatata gatggaaaat catatactcc acaagaaata
    12841 tcagcaataa tattacaaaa attaaaatca gatgcagaaa gttacttagg acaaacagta
    12901 acagaagcag ttataacagt accagcttac tttacagatg ctcaaagaca agcgactaaa
    12961 gatgctggta gaatagcagg gcttgatgtt aagagaataa taaatgagcc aacagcagca
    13021 gctcttgctt atggtatgga taaattagac caagaaaaga aaatattagt atttgactta
    13081 ggtggaggaa cttttgacgt atctatactt gaaataggag atggaacttt tgaagtttta
    13141 gctacagctg gtaacaatag attaggtgga gatgactttg accaaatagt aatagattat
    13201 ttagcagatg aatttaaaaa agctgaaggt gtagatttaa gaaatgataa aatggctctt
    13261 caaagattaa aagaagcagc agaaaaggct aagaaagaat tatcatcaac aatgagttca
    13321 aacataaact tacctttcat aactgctaca gcagaaggac caaaacactt aaatatagat
    13381 ttatcaagag ctaaatttga agaattaact agaggtttag ttgaaaaaac tatggaacca
    13441 actaagacag ctttacaaga tgcaggactt tctacaggtg atatagatga tgtattatta
    13501 gtaggtggtt ctacaagaat accagcagta caagaagctg ttaagaagtt tataggaaaa
    13561 gaacctcata aaggtataaa cccagatgag tgtgttgcag caggtgcttc tatacaagct
    13621 ggagttttag caggggatgt taaagattta ttattactag atgttactcc attatctctt
    13681 ggtatagaaa ctatgggtaa tgtaatgact aagataatag aaagaaatac tacaatacca
    13741 actaagaagt ctcaaatatt ctcaactgct gctgataacc aaacagctgt tgatatacat
    13801 gttcttcaag gtgaaagaag tatggcttat gacaatacaa ctttaggaag attccaatta
    13861 acagatatac caccagcaca aagaggaata cctcaaatag aagttacttt tgatatagat
    13921 gcaaatggta tagttaatgt ttcagctaaa gatttaggta caggaaaaga gcaaaaaata
    13981 actataacat caaacactaa tttatcagaa gcagaaatag agcaaaagat aaaagaagca
    14041 gaaatgaatg cagaagctga caaacaaaag aaagaaaaaa tagaagcatt caatcaagct
    14101 gaatctacta tatatcaaac tgaaaaaact ttaaatgaac ttggagataa aataagttca
    14161 ggtgaaaaag aggatataga aaaagctata gcagacttaa aagctgtaaa agataatcaa
    14221 gatgctacag cagaagaact taagaaagct acagatgaag taatgactaa gttccaaaaa
    14281 gtatctcaag aaatgtacca aaaagcagct caagaacaac aagcggcaca aggtgcagaa
    14341 caagcacaag acaatggacc taaagatgac aatgtagtag atgctgactt taaagaagta
    14401 gatgaagata aataagctat ataatatgta acactaaatg ataattggaa catatatgat
    14461 ttgtgaaaat catatagatg ttaaagatat atggacaata cacatcataa agaatataat
    14521 atatcataga ttatataact taaagtagaa tataagaatt aatgatagct tgtagtttat
    14581 tattctacaa gctatcgttt tgtaaaatga aggtggtgtc aatttttgag tacaaaaagg
    14641 gattattatg aagtcttagg tatcagtaaa gatgcagaag ctcaagagat aaaaaaagct
    14701 tatagaaaat tagcaatgaa gtaccatcca gatagaaatc ctggagataa agaggcggaa
    14761 gaaaaattta aagagataaa tgaagcttat gaagttctat ctgatgatac aaagagaaaa
    14821 acttatgacc agtttggaca tgatggatta aatggtcaag gtggttttgg tggtcaagga
    14881 ggatttggcg gtcaaggctt tggtggtttt gaagatatgt tcggagatat atttggtgac
    14941 atgtttggag gaagcttcgg aggtggaaga gcaagaagaa gagggcctca acgtggtgca
    15001 gatataagac agagtgttac tatatctttt gaagaagctg catttggtaa aaagatgtct
    15061 ataaaagtaa atagaagtga agagtgtgaa gaatgtaatg gaactggagc aaaaccagga
    15121 acatcaaaga aaacttgttc aacttgtaat ggaactggcc aagttagaac tgttcaaaga
    15181 actccatttg gtaatatagc aagttcaaga ccatgtagtg cctgtaatgg aactggagaa
    15241 gtgattgaat ctccatgtag taaatgtcat ggaactggaa atacaagaaa agttaagact
    15301 atagaagtag atatacctgc tggtatagat gatggtcaaa tgataaaact ttctggtcaa
    15361 ggtgaagtag gagaaaaagg tgctccaaga ggagacttat atattgtagt taatgttaag
    15421 tcacatccac tatttactag agatggaaat gatatttatt ttgaaatgcc aattacattt
    15481 gttcaagcaa ctttaggtga tgagatagaa gtacctactt tggatggaaa agttaaatat
    15541 agtgtgccag aggggacaca aactggaact gtatttagac ttaaagagaa aggaatccct
    15601 agaattagag gtaattcaag aggagaccaa tatgttaagg ttgtagttga aattcctaaa
    15661 aaattaaacg ataagcaaaa agaactattg agagaatttg cgaaggagtg tgggtcgaac
    15721 gtccacgaaa agaagaaaac cttcggacaa aagatagaag atatgtttaa gaaaaagtag
    15781 ttaaagtagc caatatataa tcatattttt gaaatcttag ttatttataa gattgtcaaa
    15841 ggagatatta tgatttttat attggctatt tttgtataaa aaataaatgt gcataaaata
    15901 aaactgaata taaataattt tctgaaaaat acttgtccta aaatatataa tgtgctataa
    15961 tataatcaaa taaaagaaag gggtgattcc agtgggaacg gatgtggcac tgccaaatga
    16021 tgaagaaaac tttattgtaa tatacaaaat aaaattagat acttttagtg ctagattaag
    16081 tactggaaaa actcatgagc tgggataatt attgtaatta cctagactca aacttttagt
    16141 agtaaaatag taatattata tagacaaggt atctaatttt taaatctttt gtatgagtta
    16201 aaatttttat aaagggtata tctgattaga atgtaattag ggaaacctag ttgtgaatta
    16261 cttttagtat atacatgtgt aaagaaatat gtatatggta ttggttattg tgctagatgt
    16321 gtatgtaaag gaatatgtgt ctagttgggt atatgtaaag aaatatactt ataatctatg
    16381 aaaaattttt agtataaatt gctaaaacat tgagattgtt ttttatagaa agttagttta
    16441 taccaactaa aaatgtgctt aagatttgtt tgatgtttaa ttataaataa ggttgtagat
    16501 aaaaaaataa gtaaagacaa aggtttaaaa tacttgcttt aaatatagtt tgttttgaac
    16561 acttgccaaa ttaaaataat taataaaata gacatcttct catagtaaaa tatactgtga
    16621 gaggatgttt ttgctttgtt cataaacttt tatgaaaaat ttgcatagtg tcacatttta
    16681 atatactata tatataataa aatatagata aaattaaatg attcaaaaga gaaaggtgaa
    16741 aagagatgta ttatataggt gtagacatag gaggaacagg tatacaagca ggagtagttg
    16801 ataactatgg gaagataatt tttaggtcag agtgtaaaac agtaattgaa aaaggctttg
    16861 aaggaatact taatgacata aaaataatga tttataagtt gttggaagat aataaactta
    16921 ctatgagtga cataaaatct attggatttg gtgttccagg ttttataaat aaagaaggat
    16981 tagtaacttg tgtaaattta aaatggaata aaaaagcttt taataaagag ttaaaaagaa
    17041 gatttccaga tgctgaaatt catggagaaa atgatgcaac agtagcagca cttggagaag
    17101 caaagtttgg aagtatgaaa ggtgctaatg ttggagtatt atatacttta ggaactggtg
    17161 taggtggagg aatagtaatt aatcaaaaag tcttttctgg agcacatgga ttgggttcag
    17221 aaataggtca tcaaattata ggagaaaact attttaattg taattgtgga aataatggtt
    17281 gtgtagaaac tttttgttca gcaactgcaa ttataaagta ttctcaaaaa ctgatagaag
    17341 aaggagaaaa gagtaggatt ttagatttgg cagagggaaa tttagaaaat gtaaatgcaa
    17401 agatggtgtt tgatgcatat agagaaaatg atttagtggc aattaaagta ataaatagat
    17461 ttaaagatta tttagcaaaa acttttgcaa acacaataaa ttctttagac ccagaaatta
    17521 tatctatagg aggaggcatt tctaaatcta gcgatataat acttgatgga atagaagatt
    17581 tggtaagaaa atttgtatta tacaaaacag aagatatagc tacaattaca tgtgcaactt
    17641 taggttcaga cgcaggaatt ataggagcag cttttttata atgtaaatga actgcttatt
    17701 ggaggcgtga tgatatatgg gtaaaacaaa tgctagaaga acgctttttt tgatagcaat
    17761 aggttcagga actatgctga atcctcttaa ttcttctatg atttcacttg ctttacatag
    17821 tatacaaaat gattttcata ttagttttac aactgtatct tggattgttt cagcatttta
    17881 cctagctagt gctattgcac aaccagtttc aggtaagata ggagatttaa taggtagaaa
    17941 ggtcttattt ttgagtggtt tgatgcttgt aattttatca gcatccatag taccacttgt
    18001 tcaatcattc tttatattga tttttataag agtaattcaa gcaattggaa caagtaccct
    18061 atatccatct ggagttgctc ttatacaaaa taatataaaa gaaagacaat cttctgcatt
    18121 agcagttcta accatttttg cttcaactac agcaggatta ggtccgacac ttggaggatt
    18181 attgttggac ttaggaggat ggcatgctat atttcttgta aatatacctg ttgttttggt
    18241 ttctatatgc ttagggtatt ttttattccc aaaagaagta aaagaaaaga aatctataaa
    18301 agaaacttta aaaaatgttt tgtcaagatt agatattatt ggcatacttc tattttcaat
    18361 tgcaatgatt tttatattac tatttttgct atctatgaag gaatctttta acttagaaca
    18421 acttgtattt ggtataatac tgatgtgtct ttttatttgg catgaattga gaacaaaatt
    18481 gccatttata gatattaagc tttttgtaag taatcctaaa ttgtcaaaag tttacttgca
    18541 atttattata ttaaatttct ttaattatat tttgtttttt ggtcttccta gttattttca
    18601 agatgcctta cattatagtg caaagtcgac tggattattt atgctattta tgtctggtat
    18661 aggtatattt atatctccat taactggaaa atggatagat aaatctggaa ctagatttcc
    18721 tcttgttaca tcatcaatct ttatgtttat tagtgcagta cttctttcag tagtttttat
    18781 ccatatacct gtaataggaa agggtattat attatcatta gcaggtatta gttatggagt
    18841 tggtaatgta gccctgcaat cctctatgtt tgaagaaagt cctcgtgaca gtatagggac
    18901 agcttctggt ctgtttcaaa ctagtcgtta tttaggttct attttagcaa ctgttttact
    18961 tggaatggta tttgaaggaa atataacatc agaacaattt cagatattag gttatgttat
    19021 ggtggtctta ggtacaataa gttttttatt aaattttata ttaaagaaag aattaagaaa
    19081 agtagaatag tggatttatg gatttaataa gtctaataac tgataagttt aaaaatattg
    19141 tttgtaaaac ataactggtt tgatatttat taattctaag ttttgaagca tagagtactc
    19201 gataagtata caatattttg aattaaaatt aagcagaaat ttgaactagt ctt
//
LOCUS       DAEPLF010000054        19094 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEPLF010000054 DAEPLF010000000
VERSION     DAEPLF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19094
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.054"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            235..384
                     /gene="rpmG"
                     /locus_tag="KR314_001667"
     CDS             235..384
                     /gene="rpmG"
                     /locus_tag="KR314_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBH0328055.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            410..631
                     /gene="secE"
                     /locus_tag="KR314_001668"
     CDS             410..631
                     /gene="secE"
                     /locus_tag="KR314_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBH0328056.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            651..1193
                     /gene="nusG"
                     /locus_tag="KR314_001669"
     CDS             651..1193
                     /gene="nusG"
                     /locus_tag="KR314_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBH0328057.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDVDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1229..1654
                     /gene="rplK"
                     /locus_tag="KR314_001670"
     CDS             1229..1654
                     /gene="rplK"
                     /locus_tag="KR314_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBH0328058.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1724..2422
                     /gene="rplA"
                     /locus_tag="KR314_001671"
     CDS             1724..2422
                     /gene="rplA"
                     /locus_tag="KR314_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBH0328059.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2474..2602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2645..3151
                     /locus_tag="KR314_001672"
     CDS             2645..3151
                     /locus_tag="KR314_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBH0328060.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3209..3574
                     /gene="rplL"
                     /locus_tag="KR314_001673"
     CDS             3209..3574
                     /gene="rplL"
                     /locus_tag="KR314_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBH0328061.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3966..4754
                     /locus_tag="KR314_001674"
     CDS             3966..4754
                     /locus_tag="KR314_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH0328062.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5225..8941
                     /locus_tag="KR314_001675"
     CDS             5225..8941
                     /locus_tag="KR314_001675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBH0328063.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRKLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLMNSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGQSFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     VEATEDEDIENLEEFTEDDLFEEEIDFESDDFDM"
     gene            8983..12468
                     /gene="rpoC"
                     /locus_tag="KR314_001676"
     CDS             8983..12468
                     /gene="rpoC"
                     /locus_tag="KR314_001676"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBH0328064.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVLVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12757..13179
                     /gene="rpsL"
                     /locus_tag="KR314_001677"
     CDS             12757..13179
                     /gene="rpsL"
                     /locus_tag="KR314_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBH0328065.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13302..13772
                     /gene="rpsG"
                     /locus_tag="KR314_001678"
     CDS             13302..13772
                     /gene="rpsG"
                     /locus_tag="KR314_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBH0328066.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13821..15887
                     /gene="fusA"
                     /locus_tag="KR314_001679"
     CDS             13821..15887
                     /gene="fusA"
                     /locus_tag="KR314_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBH0328067.1"
                     /translation="MARKFPLEKTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFINKMDILGADFYNVISMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
     gene            15901..18387
                     /locus_tag="KR314_001680"
                     /pseudo
     CDS             15901..18387
                     /locus_tag="KR314_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
     gene            18405..19004
                     /locus_tag="KR314_001681"
     CDS             18405..19004
                     /locus_tag="KR314_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBH0328068.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
BASE COUNT         7607 a         2228 c         3815 g         5444 t
ORIGIN      
        1 atggattaaa aggaataaca aaaagagggg ttaggctcct ctttttttaa catagaaata
       61 caaaaacatt gacacagcat aggattttgt gataacttat acaaggtaat gattatagga
      121 agttaataat atcttgaata ctgtagattt ttaataaata catattttaa agatattata
      181 ttcacatata ttaacaatat atattattgt attacaaagt tggaggtgta tcagatgaga
      241 gttaaagtaa ctttagcatg tacagagtgt aagcaaagaa actacaacac tactaagaat
      301 aagaaaaata atccagacag aatagaatta caaaaatatt gtagattctg taaaaagcat
      361 acaactcata aagaaacaaa atagtttttt ataaggatgt gagtatggaa tggctgccca
      421 aaaaaatgac ggtgcaaaaa ctaaaaaaag gtttagttta tttggatatt taaaagaaac
      481 taagcaagaa ttaaaaagag ttacatggcc gacaaaaaag gaactgttta aaaacacaag
      541 tatagtttta actgtagtta tttcatgcac tatattagta tggggtatag acactatatt
      601 atctggtgca ctagcgttat tactaaaata gacgaaaaga ggtaagttaa atgtcagaat
      661 tacaagaagc aagttggtat gtagttcata cctattcggg acatgaaaat aaagttaaag
      721 ctacaattga gaaagcagtt aaaacaagag gtatggaaga ctgcataaga caagtagttg
      781 ttccaacaga agaggtagtg gaaactacta aaactggaaa agaaaaaact agacaacgta
      841 aagtttatcc aagttatgtc ttggttaaaa tgataataac tgatgaatct tggtatgtag
      901 taagaaatac taagggagtt acaggtttcg ttggacctgg ttcaaaacca gtaccattaa
      961 gtgaagatga agtcaaagct atggggatag atacaacaga ccctaaagta gtaaatagcg
     1021 atgttgattt tgaaatagga gacacagtaa aagtatctca aggaccattt agtggacaaa
     1081 taggtaacat agaagaaatt gacttagaaa atagagaagt caaagtatgc ataaatgcat
     1141 ttggtaaaag gactctattt gtaatagagc ttgaaggtat agaaaaaatc taatactata
     1201 tagaaaaatc tgaggaggtg cgctcaaaat ggctaaaaaa gttataggtc aaataaaatt
     1261 acaaatacct gcaggaaagg ctactccagc gccaccagtt ggaccagcat taggacaaca
     1321 tggtgttaat ataatgggat tcacaaaaga atttaatgct aaaactgcag atcaagctgg
     1381 aatgataata ccagttgtta taactgtata tcaagataga tcattcagtt ttataacaaa
     1441 aactccacca gctgcagttt taattaaaaa agcattaaac ttaaaatcag gttctggaga
     1501 accaaacaaa aagaaagttg ctaaaatgac ttcagctcaa gtaagagaaa tagctgagtt
     1561 aaaaatgcct gacttaaatg ctgcatcagt agaagctgct atgagtatga tagctggtac
     1621 tgctagaagc atgggtgttg taatcgaaga ctaattaaaa gatacacttt ttttataaag
     1681 tgggaggcga gaagccgaaa ttaaccacaa ggaggaaaat aaaatggcta aaaaaggtaa
     1741 aagatatgca ggtgcattac aaaaagtaga tagaactaaa ttttatgatg catcagaagc
     1801 attaacatta gtttcagata tagctggagc taaatttgat gagacagtag aagcacacat
     1861 taaattaggt gttgactcaa gacatgctga ccaacaagta agaggtgcag ttgtattacc
     1921 tcatggaact ggtaaaacaa aaagagtttt agtttttgct aaaggtgaaa aagctaaaga
     1981 agctgaacaa gctggtgctg actttgtagg agctgaagaa ttagttcaaa aaatacaagg
     2041 tgaaaactgg tttgactttg atatagtagt tgctactcca gatatgatgg gtgtagtagg
     2101 tagattaggt agagtattag gacctaaagg tttaatgcca aaccctaaat caggaacagt
     2161 tacatttgat gtagctaaag ctatagatga aataaaagct ggtaaagttg aatacagatt
     2221 agacaaaact aacataatac acgttccagt aggaaaagta tcatttggtg gagaaaaatt
     2281 aactgaaaac tttactgcat taatggatgc tataataaaa gctaaaccag ctgctgctaa
     2341 aggacaatat ttaagaagta taacagtagc ttcttcaatg ggacctggag ttaaaataaa
     2401 cccagcaaga acagctgaat aataaaactg ttgacacagg ttgacaaaaa tgttattata
     2461 tcacttgtca atgtaaaaaa gaatagattt gccgtagata gtgggtgcga aagcttaata
     2521 tcccgccgag gttttagata aattgagatt actaaagctt ttgatgtcta cttttggcaa
     2581 caaaagcttt ttataatgtt gcggtaaata ttaagcctat ttggtgaagg aggtgcacat
     2641 ctatatgaga aaagctatag aaattaaatc agaagttgtt tctgagatag ttgaaaagtt
     2701 acaaaaatct tctgctgctg ttgttgtaga ttacaaagga ttaacagttg aagaagtaac
     2761 tgagttaaga aaacaaatga gagaagctgg tgtagattac aaagtataca aaaatacttt
     2821 agttagaaga gcagctaaag aagttggtat agagcaattt aatgatgaat tattagttgg
     2881 aactaatgcc atagcatttg gatatgatga tccagttgct ccagctagaa tattaaaagg
     2941 attcatggat tctcatccaa aaatgaaatt aaagatgggt attgtggaag gtgcattcta
     3001 tgatgaatct aagattgttg aaatggctaa cataccatca agagaagttc ttatcgctaa
     3061 attacttggt agcttaaaag ctccagtatc aaacttcgca tacttaatag atgcaatagc
     3121 gaaaaaggca gaaggtcaag aagaagctta attgcttaac taaaaaatta taaatattaa
     3181 tttgaaataa aattcggagg tgctaaaaat gacaatagaa caaatattag aagctataga
     3241 aaatatgaaa gttttagaat taaatgaatt agttaaagct gctgaagaaa aattcggtgt
     3301 atcagcttca gctccagtaa tggtagctgg tgcagctgct ggtggaccag ctgctgaaga
     3361 aaaaactgag tttgatgtag tattaactga cgttggttct tcaaaagttg gagttataaa
     3421 agcagttaga gaaataacag gattaggatt aaaagaagct aaagaagtag ttgacaatgc
     3481 tcctaagaca gttaaagaag gagcttctaa agaagaagct gatcaaataa aagagaaatt
     3541 agaagctgct ggagctaaag tagaagttaa gtagtttctt taaaaagata cccattgggt
     3601 atcttttttt atttataaaa aactataaag aaagtatttg gaattataaa gaaagtgttt
     3661 ttataaaata aaaaaaattt atataatagt actaaattaa gggtatcata tatggtgtaa
     3721 tggtatttta atttaacatt attatcaaat taaaatataa acaattgttg taatgtctgt
     3781 atataaagta tataattaaa ctaaaatgta aaattttaca ttttagttta aaaaaataca
     3841 cgagtgtttt ataaataaaa tgtaataaat aattaattaa caaatataaa ttttaaaata
     3901 agttagatat ttagaaaaat cgaaatttgt aggatttaaa ggacatattc aaggaggagt
     3961 taataatgga aaaattacaa ggaaaaattg cagtagttac tgcagcaaca aaaggtattg
     4021 gattagcatc agcagagata ttagcaaaaa atggagcaac tgtgtactta gcagctcgtt
     4081 cagaagaatt agctaatgaa gttataaata aaataagtgc agaaggtggt tgtgctaagt
     4141 ttgtttactt taatgctcgt gaagaagaga cctttacctc aatgatagaa gaggtagtta
     4201 aaaaagaagg taagatagat atacttgtaa acaattttgg ttcaacaaac ccttctcttg
     4261 acaaagacct tgtaactggg gatacagata atttctttga cacagtaaat actaatttaa
     4321 aaagtgtgta tttaccatgt aaagcagcaa ttcctcatat gataaagaat ggaaaaggaa
     4381 gtatagtaaa tatatcaagt ataggttcag tattacctga tttatctaga atagcttact
     4441 gtgtatcaaa agcagcaatc aactcattaa ctcaaaacat agctacacaa tatgctaaag
     4501 acaatgttag atgtaatgct gtacttccag ggcttattgc aactaaagca gcattagaca
     4561 atatgtctcc agagttcata aaagaatttt taaagcatgt tcctttaaat cgtatagggg
     4621 aaccagatga tatagcaaaa gctgttctat tctatgctag tgatgactca tcatttataa
     4681 caggggattt acttgaagtt gcaggaggat ttggtttacc tactccacaa tttgcagata
     4741 atatattagg ataattaaat tactaatatg caatgaaagc ttatgcatta gttatagtag
     4801 aaatgatatt tttataaagg ccatagatat aatttatatt ggaaaggttt gaggttcaaa
     4861 attccaagtt aaatttattt atggtcttta ttttgtatat tttaataaat tgtatatatt
     4921 tgtataaatg taattattta caatcatttt aatatggaat tataattaaa taggggggat
     4981 ggtacttgta taaaattttt tatagaaaat tagacctata aatgaaaaat atggatttta
     5041 tattggaaaa ttgtacataa tacataatat atgcttaaaa taatttacaa aaaatcaaaa
     5101 ataatgcata gtttacatat aagataaaaa atatattgaa aaatacttga cattatgcta
     5161 aaattattag atgcgttaga atgaactatt gttttatttt taattattga gaggtgaaaa
     5221 gtgaatgcca catcctgtca cgataggtaa aagaactaga atgagctttt ctaaaattaa
     5281 ggaaatagct gatgtgccaa atcttataga aattcaagta gattcctatg agtggttttt
     5341 aaaagaaggt ttaaaagaag tgtttgatga tatttcacca atagaagatt atactggtaa
     5401 tcttatattg gaatttgtcg actattctct agatgataaa ccaaagtacg atatagaaga
     5461 gtgtaaagag agagatgcta catattgtgc acctctaaag gttaaggtaa gacttataaa
     5521 taaagaaaca ggtgagataa aagaacaaga agtgtttatg ggtgacttcc ctttaatgac
     5581 agaaagagga actttcgtta taaatggagc agaaagagtt atagtaagtc aattagttag
     5641 atctcctggt gtttactatg cggaagaaag agataaaaca ggtaagagat tgatttcatc
     5701 tactgtaatt cctaatagag gagcatggtt agaatatgaa acagattcta atgatgtaat
     5761 atctgtaaga gttgatagaa caagaaaaca accagttaca gttttactta gagctttagg
     5821 aataggaaca gatgcagaga taattgacct tttaggagaa gatgagagat tatctgctac
     5881 attagaaaaa gataatacta agacagtaga agaaggtctt gtagagatat acaaaaaatt
     5941 aagaccaggt gaacctccta ctgtagaaag tgcatcatct ttattaaatg ctttattctt
     6001 tgacccaaaa agatatgacc tagcaaaagt tggtagatat aaattcaata agaaacttgc
     6061 tttatgttat agaataatga ataaaatttc agcagaagac ataataaatc cagaaactgg
     6121 agaagtattt gtaaaagctg gagaaaaaat aagctatgat ttggctaaag ccatacaaaa
     6181 tgcaggtata aatgtagtaa atctactaat ggatgatgat aaaaaagtta gagttatagg
     6241 aaataatttt gtggatatta agtctcacat tgattttgac atagatgatt taaacataaa
     6301 agaaaaagta cattatccaa ctttaaaaga aattttagat ggatatagtg atgaagaaga
     6361 aataaaagag gctataaaat caagaattaa agaacttata ccaaaacata tattattaga
     6421 tgatataata gcttcaataa gttatgagtt caatatattc tataatatag gaaatattga
     6481 tgatatagat cacctaggaa acagaagaat aagatctgta ggtgaattgt tacaaaatca
     6541 agttagaatt ggtctttcaa gaatggaaag agttataaaa gaaagaatga cggttcaaga
     6601 tatggaagct ataacgcctc aagcgttagt taacataaga ccagtttcag ctgcaataaa
     6661 agaatttttt ggtagttctc agttatctca gttcatggat caaacaaatc ctctatctga
     6721 gttaacgcat aagagaaaat tatcagccct tgggcctgga ggtctttcaa gagaaagagc
     6781 tggattcgaa gtgcgtgacg ttcaccattc acactatggt agaatgtgtc caatagagac
     6841 tccagaggga ccaaatatcg gtcttatgaa ctctctagga acttatgcaa aaataaatga
     6901 atttggattt atagaatcac catatagaaa atttgataaa gaaacatcaa cagttactga
     6961 tgaaatacat tatttaactg ctgacgaaga agatttattc gtaagagctc aggcaaatga
     7021 gccattaaca gaagatggta aattcgtaaa ccatagagtt gtttgtagaa ctgtaaatgg
     7081 tgctgttgaa atggttcctg aaagtagagt agattacatg gatatatctc ctaagcaggt
     7141 tgtatctgtt gccactgcta tgataccttt cttagaaaat gatgatgcca accgtgccct
     7201 tatgggagcg aacatgcagc gtcaggcagt gcctctagtt agaagagaag caccaattat
     7261 aggaactggt atagaataca gagcagcaaa agactctgga gcagttgttg ttgctagaaa
     7321 ttctggtata gcagaaagag taacagcaga tgaaataatt ataaaaagag aagatggaaa
     7381 cagagataga tataatctac ttaagtttaa acgttcaaac tcaggtactt gtataaatca
     7441 aacacctata ataaataagg gtgaccagat aataaaaggt gatgttatag cagatggtcc
     7501 agcaactgat ttaggagaag ttgcacttgg aagaaactgt cttatagcat tcatgacttg
     7561 ggaaggttat aactacgagg atgctatatt aataaatgaa agattagtta aagaagatag
     7621 attatcaaca attcatatag aagaatatga atgtgaagct agagatacaa aactagggcc
     7681 agaggaaata actagagata tacctaatgt tggagatagt gcaatcaaaa acttagatga
     7741 tagaggtata ataagaatag gtgcagaagt agactcaggt gatatattag ttggtaaggt
     7801 aactccaaaa ggagaaactg aacttactgc tgaggaaaga ttacttcgtg cgatattcgg
     7861 agaaaaagcc agggaagtta gagatacttc acttaaagta cctcatggtg aatctggtat
     7921 aatagttgat gtaaaagtat ttactagaga aaatggagat gatttatctc caggagtaaa
     7981 tgaattagta agatgttata tagctaaaaa gagaaagata aaagttggag ataagatggc
     8041 tggtcgtcat ggtaataaag gggttatctc gagagtatta cctgaagaag atatgccatt
     8101 catggaaaat ggaacgccat tagatataat acttaaccca caaggtatac catcacgtat
     8161 gaacatcggt caggttctag aagttcattt aggacttgct gctaagacat taggatggta
     8221 tgtagcaaca tcagtattcg atggtgctaa tgagtatgac ataatggatg ctcttgaaga
     8281 agctggatat cctagagatg gtaaattaac tttatatgat ggtagaacag gtcaatcttt
     8341 tgacaataga ataactgttg gatatatgta ttacttgaaa ctacatcact tagtagatga
     8401 aaaactacat gcaagaagta ctggaccata ctcattagtt actcaacagc cactaggtgg
     8461 taaagcacaa tttggtggac aaagatttgg agagatggag gtttgggctc tagaagcata
     8521 tggagcggct cacatacttc aagagatact tactgttaag tctgatgatg ttgtaggtcg
     8581 tgttagaact tatgaagcta tagttaaagg tgaaaacata cctgagccag gaatcccaga
     8641 atcatttaag gttcttataa aggaacttca aagtttatgc ttagatgtaa aagtattgac
     8701 agatgaagac caagaaatag aagtaagaga atctgtagat gaagatgata cgataggtga
     8761 atttgagcta gacgttgtaa atcacatggg agaagttgaa gaaagtaata taatagaaga
     8821 aattgaagat gattttgtgg aagccacaga agatgaggat atagagaatt tagaagaatt
     8881 cactgaagat gatttatttg aagaagaaat agattttgaa agtgatgact ttgatatgta
     8941 aatttaatta atgtaaatct caaatagaag ggagagaact ccttgtttga attaaacaat
     9001 ttcgagtcga taaaaatagc attggcttct ccagaaaaaa taagacaatg gtctagagga
     9061 gaagttaaaa agccagaaac tataaattac cgtactttaa agccagaaaa agatggtctt
     9121 ttctgtgaaa ggatatttgg accacaaaaa gactgggagt gtcattgtgg taaatataga
     9181 agagttagat ataaaggtgt agtttgtgat agatgtggag tagaagtaac taaatcaaaa
     9241 gtaagaagag agagaatggg acatatagag ctagctgctc ctatgtctca catctggtac
     9301 ttcaaaggta taccaagtag aatgggactt ttacttgata tgtcaccaag atcgttagaa
     9361 aaaatactat atttcgcctc atatgtagta gttgatccag gtgaaactgg attgaatgaa
     9421 aaacagttac ttacagaaaa agaatacaga actgctcttg aaaagtatgg atatactttt
     9481 actgtgggta tgggtgctga agctgtaaaa acattactac aaaatataga tttagaacaa
     9541 caaagtaaag acttaagagc ggaattaaaa gatagtacag gacaaaagaa agtaagaaca
     9601 attagaagat tagaagttgt agaagcattt aaaaaatctg gaaataaacc agaatggatg
     9661 attttagatg caataccagt aataccgcca gatttaagac caatggtaca acttgatggt
     9721 ggaagatttg caacttcaga cctaaatgat ttatacagaa gagttataaa tagaaataat
     9781 agacttaaaa gactattaga gcttggagct ccagatataa ttgtaagaaa tgaaaaaaga
     9841 atgcttcaag aagctgttga tgcattgata gataatggta gaagaggtag acctgtaaca
     9901 ggacctggaa atagaccact taaatcttta tcagatatgt taaaaggtaa gcaaggtcgt
     9961 ttccgtcaaa acttacttgg taagcgtgtt gactactcag gacgttctgt tatagttgtt
    10021 ggaccagaac ttaaatttta tcaatgtgga cttccaaaga agatggcatt agaattattc
    10081 aagccatttg ttatggataa attagttaaa gaaggttatg cacataatat aaaaagtgcg
    10141 aaatctatag tagaaaaagt taagccagaa gtttgggatg ttttagaaga tgttataaaa
    10201 agtcatccag ttcttcttaa ccgtgcaccg actctacata gattaggtat acaagcattt
    10261 gaaccaatct tagttgaagg taaggctata aaactacatc ctcttgtatg tacagcttat
    10321 aatgcagact ttgatggtga ccaaatggca gtccatgtac ctttatcagt agaagcacaa
    10381 gcagaagcaa gattcttaat gctttctgta aataatatac ttgctcctaa agatggttca
    10441 cctataacta ctccatctca ggatatggtt ttaggttgct attatctaac aatagaagcc
    10501 caagatggag ctaaaggaac aggtatggta tttaaagact ttaatgaact attacttgct
    10561 tattacaata aatcagttca tctacatgca ttagtgaaac tgaaggtaac acttgaagat
    10621 ggaagaagtt cattggttga aagtactgtt ggtagattta tatttaatga aaatatacct
    10681 caagacttag gttttgtaga tagaaaagaa aatccatttg cacttgaagt tgatttctta
    10741 gcagataaaa aatctcttgg taaaataata gataaatgct ttagaaaaca cggaaataca
    10801 gaaacagctg aattactaga ttatataaaa gctctaggat ttaaatattc tacattaggt
    10861 ggtataacag ttgctgttga tgatatgagt gtcccagaag aaaagaaagt atttatagct
    10921 gaggcagaag ctaaagttga taagtatgaa aaagcataca gaagaggtct aatctctgat
    10981 gaagaaagat atgaaaaagt tatagagaca tggacagaaa caactgataa agttactgat
    11041 gctcttatgg gtggactaga tagattaaat aatatatata taatggcaca ttcaggagcc
    11101 agaggttcta aaaaccaaat tagacagctg gcaggtatgc gtggtcttat ggcaaatgca
    11161 tctggtaaaa cagtcgagat accagttaaa tctaatttcc gtgaaggttt atcagtacta
    11221 gaatacttta catcttcaca tggtgccaga aaaggtcttg ccgatacagc tatacgtaca
    11281 gctgaatctg gatatttaac aagaagactt gttgatgtaa gtcaagatgt tattgtaagg
    11341 gaaatagatt gtggaacaga agatactaca gagatttatg ctataaaaga aggaaatgaa
    11401 gttatagaag agatatatga tagaattgta ggaagatata ctatagaccc tatattaaat
    11461 cctgaaactg gagaagttct agttgaggct gattcaatga tacaagaaga tgaagcagaa
    11521 actatagtag ctttagggat tgaaaaaatt aggataagaa cagttcttaa ctgtaaaact
    11581 aatcatggag tttgttctaa gtgttatggt agaaacttgg caacaggaaa agaagttaat
    11641 ataggtgaag cagttggtat aatagcagct caatctatcg gtgagccggg tactcagctt
    11701 acaatgcgta cattccatac tggaggagtt gcaggagctg atataactca aggtctacca
    11761 agggttgaag aattatttga agctagaaaa ccaaaaggat tagctgtaat aactgaagta
    11821 tctggtagag ttgaaattga tgaaacagga aagagaaaag aagtaaatgt aataccagaa
    11881 gaaggcgaga ctcaaacata tgtaattcca tatggttcta gattaaaagt taagcaaggt
    11941 caaatgctag aggctggaga ccctctaaca caaggattta taaatcctca tgatatagta
    12001 agagtaaatg gtgttaaggg agttcaagaa tatatagtta aagaagttca aagagtgtat
    12061 agacttcaag gggttgatgt taatgataag catatagaag ttatagtaag acaaatgcta
    12121 tctaaagtaa aagttgaaga cccaggagat acagatttat tgccaggtgg atatgaagat
    12181 gtattaacat tcaatgaatg taataaagat gctatagata aaggtctaag accagcagtt
    12241 gctaaaagag ttttacttgg tataactaaa gcatctcttg caactgattc attcttatca
    12301 gcagcttctt tccaagaaac aacaagagta ttaacagagg cagcaataaa aggtaaagaa
    12361 gaccacttaa taggacttaa agaaaatgtt atattaggta agttaatacc agcaggaaca
    12421 ggaatgaaga aatatagaaa tatagctgtt gaaaaaattg aagattaata gcatatagag
    12481 taaagaaatc ttgacaacca ttatttgtgg tgttaaaata gtaaagtgtg ttgtttaaaa
    12541 gtacactaag ggcttgaaca agttcttagt gacttttaga tggcaataaa tgagaactaa
    12601 aaagcatcaa gctagaaaag tgctaatatc acatatttat gtgttataga cattaaattg
    12661 taagtaacga atgttacaat cagtcagtat gaatattcat attgatgtag ttggcggtca
    12721 ctgataagat cgaaaatttg gaaggaggtg cagatgatgc caacaattaa ccaattagtt
    12781 cgtaaaagta gaaaagcgct agagaagaaa tctactgctc cagcattaca aaaaggttac
    12841 aactctttaa ataagaaagt aactgacgct agtgccccac aaaaaagagg ggtttgtact
    12901 tcagtaaaaa cagttactcc taagaagcct aactcagctt taagaaaagt tgccagagtt
    12961 agattaacta atggtataga agtttctgct tatataccag gagaaggaca caacttacaa
    13021 gagcatagtg ttgttcttat aagaggagga agagtaaaag accttccagg ggttagatac
    13081 catatactaa gaggtactct agatacagca ggtgttgata agagaagaca atcaagatct
    13141 aaatacggtg cgaagagacc taaagaagct aaaaaataaa acaattaatg attgattata
    13201 gttcggtgct ttaaatatat atatatttac ggcatcacgg cattaaatga tttatgtcga
    13261 gtacctatga tttaagttaa ataattaagg agggaagcga aatgccaaga aaaggtaata
    13321 ttccaaaaag agaagtttta ccagatccta tgtatgggag taaggtagtt actaaattaa
    13381 taaataatct aatggtagac ggaaaaaaag gaaagtctca aagaatagtt tatgatgctt
    13441 tcgcaattgt tgctgaaaag acaggagaag aagctttaga agtatttaat aaagcaatgg
    13501 ataatataat gcctgtttta gaagtaaaag caagaagagt tggtggggca aattaccaag
    13561 tgccaatcga agttagacct gaaagaagac aaacattagg tttaagatgg ctagtaaaat
    13621 atactagagc tcgtggagaa aaaggaatgg ttgaaaagct agctaaagag ataatggacg
    13681 cagctaacaa cactggagct tcagttaaga aaaaagaaga tactcataaa atggcagaag
    13741 ctaataaagc atttgctcat tacagatggt aggatatata gatatcttat aaattcaagg
    13801 atatcataag gaggataacc atggctagaa agtttccttt ggaaaaaact aggaatatag
    13861 gaatcatggc tcatatagat gcaggaaaga ctactactac agaaagaatc ctgttttata
    13921 ctgggcaaac tcataaaata ggagaaactc atgaaggagc ttctcaaatg gactggatgg
    13981 agcaagaaaa ggaaagaggt ataacaataa cttctgccgc tactactgct tcatggaaag
    14041 accatagaat aaacataata gatactcctg gacacgtgga cttcacagtt gaggttgaaa
    14101 gatctctaag agttcttgat ggttcagttg ctgtattctg tgcaaaaggt ggggttgagc
    14161 ctcaatctga aaatgtatgg agacaagctg aaacttatgg agtacctaga atagctttta
    14221 taaacaaaat ggatatatta ggtgcagatt tctacaatgt tataagcatg atgaaatcta
    14281 gattaaattc taatgcagtt cctatgcaat taccaatagg taaagaagat tcatttatag
    14341 gtataataga cttattaaaa atggatgctg ttatatacaa agacgaccta ggggttgaaa
    14401 tggaagaaac agatatacca gaagatatga aagaattagc tgctgagtgg agagaaaaat
    14461 tagttgaatc agtagctgaa actgatgaag aattaatgat gaaatacctt gaaggtgaag
    14521 aacttactat agatgaatta aaagttgcta taagaaaagc tactatagct tgtgaaatga
    14581 acccagtttt ctgtggtaca gcttatagaa acaaaggtgt tcagttagta atagatgcag
    14641 ttttagatta cttaccagca ccaactgata taccagctat aaaaggtata ttagcagatg
    14701 gagaagaagc tgaaagacat tcttctgatg aagagccatt ctctgcatta gcattcaaaa
    14761 taatgactga cccattcgtt ggaaaactag cgttcttcag agtttattct ggaacattag
    14821 aatcaggttc ttatgtttta aatgcaacta agaataagag agaaagaatt ggtcgtatat
    14881 tacaaatgca tgctaataca agagaagaaa taactaaagt gtatgctgga gatatagcag
    14941 cagcagtagg attaaaagat actactactg gagacacatt atgtgaccct gctaatccaa
    15001 taatacttga atctatggaa ttccctgagc cagttatatc tgttgctata gaaccaagtt
    15061 ctaaagcagc tcaagaaaaa atgggtatag ctcttcaaaa attagctgaa gaagacccaa
    15121 ctttcacagt taagactgac caagaaacag gacaaactat aatatctggt atgggtgaat
    15181 tacacttaga gataatagta gatagattat taagagaatt taaagtagaa gctaaagttg
    15241 gtgcaccaca agttgcatat agagagacta taactcaacc agttgatgtt gaatacaagt
    15301 actctaagca atcaggtggt agaggacaat acggtcacgt taagattaga gttgctcctc
    15361 aagagccagg tgaaggatac aagttcacaa acaaaactgt tggtggatca gtaccaaaag
    15421 aatatgttgg tccagttgac atgggtatac aaggagctat gcaatctggt atagttgctg
    15481 gatatccagt tgtagacgtt gctgttgagt tatatgatgg ttcttaccat gaggttgact
    15541 cttctgagat ggcgtttaag atggctggtt caatggcatt caaagatgct atgaagaaag
    15601 gtaatgcagt tcttttagag ccttacttca aagtagaagt tgttactcca gaagattaca
    15661 tgggagatgt tatgggtgac ttaaactcaa gaagaggatt aatccaaggt atggaagcta
    15721 gaagtggtgc acaagttata aatgcattcg ttccactttc agaaatgttt ggatactcaa
    15781 cagacctaag atcttcaact caaggtcgtg caacttacac aatgatattc gatcactatg
    15841 agcaagttcc agcttcagtt gctaagaaaa tagctgaagg aaaataattt aaaaataatt
    15901 aataatataa atataaaaaa agaatataat atatttgact caaaagaaga aaagaagaag
    15961 gaaagagaaa ccttaaaaat tttaaatgaa gaagaaaaaa attatgaaga attattaaaa
    16021 tacaaaacta tttgggagaa taaagaagga tttacatatg aatatagacc tggtcaatat
    16081 gagcttacta aaacaataag agagttgttt agaaatagcg aagatgaaga aaaaattgca
    16141 tgtatagagg caccaactgg tataggtaaa agtgtaggat atttattgcc tgcaatatta
    16201 gaggctagaa ttaataaaaa aagattatta atttctactg acacaaagga attgcagata
    16261 caattaataa ataaagatat tccaaatgtg ttagattctt tgggtctaaa tggaaaagta
    16321 agttacggat atataaaggg aaaaaataac tatatttgta tagatagact ggaagcatat
    16381 atagatgatt atgagtctca aaaccctact aaaggagaaa ttttatcttt aatatttttg
    16441 aagagattag ttgaaggtgg caagtatgga gatatagaag aaataaatta tttagtgttt
    16501 gacaacttca aggaaataag tacccactta agaaatgtaa gttgtgaccc aaatatgtgc
    16561 agacctaaaa aatgcaaaaa agattgttta tataaaaaca gaatagaaga attaaaagaa
    16621 gaacatataa cagtagtaaa tcattcacta ttagctaaat ggccgtataa ggatgaaaaa
    16681 cctttagaaa atataatagt agatgaagca cataatttga ctgaaaaagg atatgatttc
    16741 ttttcaagca ttataaattc aaagtcttta aggtatttat tacaagaaat ttatccttac
    16801 gagtttattc aaaatagtag ctttatatat aaaaaatata gtagaaatat gagaaaaata
    16861 aaggcttttg ataaatttta taatgtatta aaaattggaa gagaagataa acaaaaaatt
    16921 gctagaagta taaatctcat tattgaagag atagattcaa tattaaagtt tggtaattgt
    16981 aatgaatata ataatgtaag taattataat ttaagatggg aactgaattt gcaaatagat
    17041 gaaattgtgg gaaaactaaa aaaggatggt atagatactg aaataagtta tagagcctat
    17101 agtgaaaaga taaagttgag ttgtgagaaa ataataaaaa atttagtttc aataataata
    17161 ataatttata ggaatattga tgatgatagt atagataaag aagcagatat atataaattt
    17221 ggaaaggcaa agacaagaga tttagaggat ataaagatta tatttgaaat ctttttagag
    17281 tatgatgaaa aggatgatta tgcaaggata gttgaaattg ataagaatta taatgacttt
    17341 gaatttcgag tagtaccatt aaaaatagca gatttatttg aagaaaatat tttatctcaa
    17401 ttagaaaaag gaatattttt atcagcaact ttgagtttat ctgaaagtat gtcttatttt
    17461 aaaaatacat taggaataga cagagttaag aatgtggaaa agattataga accaattttt
    17521 gattataaaa atagagtctc tgtagttgga ttttctgata tttgtgagta cagaaattct
    17581 gaatttccaa atgagatgag taaaattata tctaatatat caaagattac agaaggtcat
    17641 acattagctc tttttaatag taaagataga caagaaaaaa cttatgaaat attgaaaaaa
    17701 tacttacata gttttaactt agaaatttat gctgataaaa aggggattag acaccttaac
    17761 gatttaaata gaaaatgtgt agtacttggc tcaaaaggat gttttgaagg cgtagacatt
    17821 ccaggggatg gtttggtttg tgtaacttta gacaagcttc cgaatctaaa tccaaaagac
    17881 cctttgtatt ttactataat gaagaagtat ggtatagact actatactat aaattatcct
    17941 caaatgacca taaaggtaaa gcaagctatg gggagaattt taagaagcaa atatgactat
    18001 ggatgttttg tgatttttga tgtaggaact aatataagtg ttttaaagag attagagaaa
    18061 gatttacacg attgtaaaat ttcaaaggtt aatagcaatg agttttatac ttatataagg
    18121 cgacatttaa ataaatctag aagtttaata ctaaaatctg ttatatttga tactataaaa
    18181 gcgttgaatg ttgatgctaa aatggataac aatgatgttg ataaagatat tataaaaaaa
    18241 gatataaatg aaaatataag acaaagagct gtaaaaggag aagtgtacca tattgatatt
    18301 ataaaaaaag atatgaaagt aaaatatttt gatagaaact atcttataaa tttagatata
    18361 tttatgagag aagaagataa aaattaagag tgggagtgag atatttggca aaacaaaagt
    18421 tttatgctgt gaaaaaaggt aaaaatatag gtgtatataa tacatgggat gagtgcaaaa
    18481 aacaagtaaa tggattctca ggagctgaat ataagagttt ttctactttt caagaagcta
    18541 aagaatatat tgatgggtca gaaaaattga gttttcaaga agataaggag tttatagaag
    18601 catatgtaga tggaagttat gaacattcag taaaaatgta tggttcgggt gttgtgattt
    18661 taaaaaataa tgaagttata aaaacttata gtgaaaaagg aaaagagaaa actttagtaa
    18721 gtatgagaaa tgtagctgga gagatagagg cttctaaaat tgcaatgcag tactgtattg
    18781 acaataatgt tcaaaacttg attttatact ttgactatga agggattgaa aagtggtgta
    18841 ctggagtttg gaaaacaaat aaagaaggaa ctatagcata taaaaatttc tatgacagta
    18901 ttaagaataa gcttaatgta aaatttacaa aggttaaagc tcattctgga aataagtata
    18961 atgaggaagc tgataaatta gctaaaaaag ctattggtgt ataataaaaa aatatttata
    19021 cataattaca tggtataaga gtatatctaa attcctactt tataattaaa caacttgtaa
    19081 ttaaacaact tgta
//
LOCUS       DAEPLF010000055        19034 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEPLF010000055 DAEPLF010000000
VERSION     DAEPLF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19034
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.055"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            80..451
                     /locus_tag="KR314_001682"
     CDS             80..451
                     /locus_tag="KR314_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0328069.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            583..1857
                     /locus_tag="KR314_001683"
     CDS             583..1857
                     /locus_tag="KR314_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH0328070.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSNQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKSKNNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            1968..2912
                     /locus_tag="KR314_001684"
     CDS             1968..2912
                     /locus_tag="KR314_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328071.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            3128..4465
                     /gene="rsxC"
                     /locus_tag="KR314_001685"
     CDS             3128..4465
                     /gene="rsxC"
                     /locus_tag="KR314_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBH0328072.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            4488..5465
                     /locus_tag="KR314_001686"
     CDS             4488..5465
                     /locus_tag="KR314_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBH0328073.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            5465..6034
                     /locus_tag="KR314_001687"
     CDS             5465..6034
                     /locus_tag="KR314_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBH0328074.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQENNEL
                     RKAVLPEAKEFKEMDKSTYKDLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            6047..6640
                     /locus_tag="KR314_001688"
     CDS             6047..6640
                     /locus_tag="KR314_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBH0328075.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            6655..7230
                     /gene="rsxA"
                     /locus_tag="KR314_001689"
     CDS             6655..7230
                     /gene="rsxA"
                     /locus_tag="KR314_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBH0328076.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            7246..8217
                     /locus_tag="KR314_001690"
     CDS             7246..8217
                     /locus_tag="KR314_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBH0328077.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            8437..9021
                     /gene="maf"
                     /locus_tag="KR314_001691"
     CDS             8437..9021
                     /gene="maf"
                     /locus_tag="KR314_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBH0328078.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            9066..9725
                     /gene="radC"
                     /locus_tag="KR314_001692"
     CDS             9066..9725
                     /gene="radC"
                     /locus_tag="KR314_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBH0328079.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            9846..10856
                     /locus_tag="KR314_001693"
     CDS             9846..10856
                     /locus_tag="KR314_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBH0328080.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            10866..11759
                     /gene="mreC"
                     /locus_tag="KR314_001694"
     CDS             10866..11759
                     /gene="mreC"
                     /locus_tag="KR314_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBH0328081.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKDMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            11774..12262
                     /gene="mreD"
                     /locus_tag="KR314_001695"
     CDS             11774..12262
                     /gene="mreD"
                     /locus_tag="KR314_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBH0328082.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            12274..15252
                     /locus_tag="KR314_001696"
     CDS             12274..15252
                     /locus_tag="KR314_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBH0328083.1"
                     /translation="MEENKQVDRLKIIKIVMSVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKMNVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIVNGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            15350..16033
                     /locus_tag="KR314_001697"
     CDS             15350..16033
                     /locus_tag="KR314_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBH0328084.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYENESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            16057..16854
                     /gene="minD"
                     /locus_tag="KR314_001698"
     CDS             16057..16854
                     /gene="minD"
                     /locus_tag="KR314_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBH0328085.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            16872..17156
                     /gene="minE"
                     /locus_tag="KR314_001699"
     CDS             16872..17156
                     /gene="minE"
                     /locus_tag="KR314_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBH0328086.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            17241..18371
                     /gene="rodA"
                     /locus_tag="KR314_001700"
     CDS             17241..18371
                     /gene="rodA"
                     /locus_tag="KR314_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBH0328087.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            18412..18825
                     /locus_tag="KR314_001701"
     CDS             18412..18825
                     /locus_tag="KR314_001701"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBH0328088.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
BASE COUNT         7307 a         2176 c         3833 g         5718 t
ORIGIN      
        1 ttttaactta atatgattta aataaattta taataatgat ataatataaa aaaaataaaa
       61 attggagagt gatttcaaaa tgaagtttaa tttttgccat aacaatttca atgttactaa
      121 cttagaaaaa agtttagatt tttataaaaa agctttaggg cttaaagagg taaagcgtaa
      181 ggaagcagaa gatggaagtt ttatattagt ttatttagga gatggtatta ctagtcatac
      241 attagaatta acttggctta gagattggga cagaccatat aatttaggag acaatgaatt
      301 tcatctggct ttagaagttg aggagtttga tgaggcaaaa aaacttcata aagatttaga
      361 ttgcatatgt tttgaaaatg aaagtatggg aatttatttt atagcggacc cagataatta
      421 ttggattgaa atacttccta aaaatcatta attttaagga tatgtttata atttctttac
      481 aattaataag agaataagtt atcattaaat tgtagggact aaaaatggat aataatgtta
      541 tgaatttatg actataaaaa tttgttgggg ggagtgatag tagtgaagaa agctatagca
      601 gctttaggaa ttggggcagt agcagtatct gttagctcca taaatgcaag tgctcttgaa
      661 aagggaacag taacagcaag tgccttgaat ataagaagtg gaccaagttc tgattgtgat
      721 aaggtagcaa aattatacaa gggaaaaact gtagagattt tagaaaagtc taatggatgg
      781 tataaagtaa gggtatcgag ttctgttgtt ggatggggaa gcgcaaaata catatcaaca
      841 agtggttcat ctgaaggtac atcaaatcaa aataatccaa cttcaagtgg gacaactatt
      901 agtggaaatg gtaaagtaaa tgtgagttct agactgaatg taagaagtgg tgcaggtact
      961 aactattcat tagttggtaa agcaaataat ggagatgtag taaagttact agaacaaagc
     1021 aatggatggt ataaaataaa actatcaaat ggagttactg gatgggctag tagccaatat
     1081 atctctaaaa catcagagga tgttggaacg aataattcta gtaattcaaa tagtactaat
     1141 aattctgata aaaaaccatc tagcgaagaa tcaattgaag gcaagaatgg taaggtaaca
     1201 tctgctgtga gtttaaatgt aagaagtggt cctgggacaa gctattctat aataggaaaa
     1261 ttaaatggtg gagatgttgt tgaattaaaa tccaaaaata atggttggta taaggtaaag
     1321 ttatcgagtg gaacaattgg atgggtgagt gccagttata tttctgagac taatgaaggt
     1381 acaaaagaaa aaccaaattc atcatctaac caaaattctc aatctaatag taactcaaag
     1441 ccatcattta ctggaaattc agataaatct acagcaaaag gttcaacaat cgtagatttt
     1501 gcatatactc ttattggaat accatatcaa tggggagctt caggacctga taaatttgac
     1561 tgttctggat ttacacagta tgtttttaaa cattctgtag gcgtttctat tcctagagtt
     1621 tctagagaac aggctaattt tggaagtgca atttctatgg ggaattatgc accaggagat
     1681 ttagtttatt ttgacactga tggtgatgga acaactaatc atgtaggtat atatgtagga
     1741 aatagtaaat ttatccattg tagtggtact cagactaatc ctaataaggt taaagtggat
     1801 aatttaacta gttcatattg gtctaaagta ttacttggtg caagaagatt tgtttagtaa
     1861 gtacaatttc ttattaaaaa attagaagaa ataattattt atattcataa attgtgactc
     1921 ttaaaaatat actttattaa taatataatt ttgggaggga caaactaatg aatagaagaa
     1981 aaacgatata cagaggattt aataataata gacataagat tgatttacgt aaatatgtta
     2041 taacgattgc ttgcttgtgt ctaattggct attattctta tacaaaaata aaagatagca
     2101 agatattgga gtatgtatcg gctaaaatac catttctaaa taattcatca gatataactt
     2161 ataaagatat atctgatgaa ttaaattcaa taaaaaaagg gaaaaaatct aaatcacaaa
     2221 ccaattcaga tgataaacaa gaaactaatc cagaaaaagc ggtaaataac actaaagagc
     2281 cagaagaagt caaattagct actatagaag gctgggatat gtacactatt caagttgctg
     2341 caatagataa taatgatgat ttgaaaaaaa tacagacatc attagttaat aatgacatac
     2401 ctttttcagt tatggagaag gatggagtaa aaaaaataca gacctattcc tcttttgatg
     2461 aaaatgatgt tagaaagcag ataagcagtg taagaaaggt gtttccagat gcatttttat
     2521 cacacttaga tgcacctatg ttgtctttag agtatacaag taactacgct tatatagaga
     2581 gtatatctaa agaattaaat aaattaataa caaactttaa agaagaatca agtttttggt
     2641 cgaatgctga aaataatgta gatatggaaa aatataatac tattttgaca aatagaaagg
     2701 caatatcaca aaatatatca aaagaagctg aaaagattga ctattctgaa atgcgtttat
     2761 ttaaagataa tttaatagag tatgtaaaga atgtgaatga aaaaattgat acagcttcaa
     2821 aagcagcaaa tgaagaaaaa tatagtgtaa gtaaaagtct gcttttatca tccatgcagg
     2881 agtactctat gtttataaat tctataaaat aaaatacaga aaatttggac tttagaaagc
     2941 tatatgaaaa ggattgttta attaagcaat ccttttcctc agtttgactt aatatagagg
     3001 tattgattta taaaataaat tataaaaata cggatatttt tttaacaatt gttaagattt
     3061 tttaatatat atagtatact tatagaaggc agaaaaaatg tttttttata gataaggggg
     3121 taaaacaatg aaactcttaa cttttaaggg agggatacat cctccatata gaaaagagta
     3181 ctctaacaca aaggcacttg aaaaagctca agctccaaag atagtttaca ttcctcttca
     3241 gcaacatata ggggcaccag ctaaacctat tgttgaagtt ggtgatgaag taaagtttgg
     3301 gcaaaaaata ggtgaacaac aaggttttgt ttcatgtaac gttcattctt cagtatcagg
     3361 taaagttatt gctattgaac aacatgaagt accaggaggt tcagctcaat gtgtagttat
     3421 cgaaaatgac tttaaggaag aattacatga gagtgttcaa ccaaaaggtc agttagagga
     3481 tttgagcaaa gaagatatag taggtatcat aaaagaggca ggaatagtag gtatgggtgg
     3541 agctacattc cctaaccatg taaaagtatc tcctccacca gatagcaagg cagaagtagt
     3601 tatattaaat ggtgcagaat gtgaaccata cttaactgca gaccacagat taatggttga
     3661 aaatccagaa gatgttgtgt ttggtttaag agcattaatg aaggtattag atgtcaaaaa
     3721 aggatttata ggaattgaaa cgaataaacc agatgctata gaagcaattc aaaatgtagc
     3781 taaagactat agtgaaatcg aagttgttgg ccttcaagtg aaatatccac aaggagcaga
     3841 gaaacaactt atttatgcgt gtacaggcaa agaagttcca tctggtggat taccaatagc
     3901 agcaggtgca gttgttgaca atgttgccac agcagcacaa atagctaagt ctattaaaac
     3961 aggtatgcct ttggttgaaa gaatcactac aataacaggt agttgcatca aagagccaaa
     4021 gaacctaata acaaaagttg gaacattagt ttcagaaata atagaacaat gtggtggatt
     4081 taaagaagat aagaagattg gtaaggttat aatgggtgga cctatgatgg gaatagctca
     4141 atatactact gaaatagcga ctaacaaagg ttcttcagga attttatgct tggatgagga
     4201 agaatcacgt actccagata ttcaaaactg tttaagatgt ggaagatgta cagatgtatg
     4261 tccatcattc ttacaaccac ttttcataag tgcatattct ttaaaagatg actatgacac
     4321 agctgaatat catagagcaa tggattgtat agaatgtggg tcttgttcat ttatttgtcc
     4381 agcaagaaga ccattacttc aatctataag atcagcaaag agagaaatag gagcaaagag
     4441 aagaaagcaa gctgctcaga aataaagaaa gttaaggggg aaaaacgatg gaaaataagt
     4501 tgatagtatc atcttctcct catgtgagaa gtaatgaaga tacttcatat ataatgaaac
     4561 aagttattat agcactcctt ccagcagcag tagcaggagt atacttcttt agacttaatg
     4621 ctttgagtgc tatgtttttt tgtatacttg gtacagtagg tactgaattt ttatatcaaa
     4681 aatttacgaa gcaaaaaagt actataggag atttttcagc tgttgtaaca ggattattat
     4741 tagcatttaa cgtaccagca tcacttcctt ggtggatgtg tctagtagga ggaatatttg
     4801 caatattagt agttaaaatg gtatttggtg gaattggatg taactttgtc aatccagcac
     4861 ttgctgcaag agcattttta ttagcatcat tcccagtagc aatgactgct tggactcaac
     4921 caggtgttaa ctggataggt aaaaatttag atgcagttac tacagcaact ccattaagct
     4981 ttttgaaaaa tggagcagca ggactggcag acctttctag caatggaatt agtcttgctg
     5041 acatgatgat agggaatatt ggtggatgta taggtgagac atcagcaata ttattattat
     5101 taggtggagt atacctaatg tataaaggca taataaatta tgttatacca gtattttaca
     5161 tagcaactgt atttatatta acattccttt taggtggatt taatataact ttcgcaatat
     5221 atcaactgtt tgctggagga cttatgttag gtggattctt catgcttacg gattatacaa
     5281 cttcgcctat gactaaaaaa ggtcaaataa tatacgctgt attagctggt cttataacaa
     5341 ctgttataag aatgtatggt ggatatccag aaggtgtatc ttactcaata ctattagtga
     5401 actgtcttgc tccacttata gataagtttg ttagaaatag agtatttggg gaggtggcta
     5461 aataatgaac agtatggtga gattaggtgg tactttactt gctataagtg caatagctgc
     5521 attagctctt ggagctacta atcaggttac agctccagta atagaacaaa gaaatataca
     5581 agaaaataat gaacttagaa aagcagtcct tccagaagca aaagaattta aagaaatgga
     5641 taaaagtaca tataaagacc ttggagatgg tcttatagct gaagtgtatg aaggtttaga
     5701 tggttctgaa gtggttggat atactttaaa agctaaacca agtggatatg gtggagaaat
     5761 tgaagttatg gtaggtatat catctgaagg tcaagttaca ggtgttgaca ttggtaatat
     5821 gtcagaaaca gcaggtcttg gagcaaaagc taaggatgat gcatttaagg gtcaatacaa
     5881 aggaaaaact gctgagcctc ttgaagtagc aaaaggaagt acaacagcag ataatcaaat
     5941 attagcaata tcaggtgcaa ctattacatc tacagcagtt actacaggag taaatgctgc
     6001 tatagatgtt ttcaatagtg ctcttaataa ataaggaggg ttaattatga atttagctaa
     6061 agtatttaaa aatgggctaa tagatgagaa cccaacattt gttcaagtta taggtatgtg
     6121 tccaacacta gccgttacaa cttcagcaat aaatggaatt ggtatgggtc tttcaacagc
     6181 agcagtttta atatgtgcaa atttagttat atctttaata agaaaaatta caccagataa
     6241 gataagaata cctatattta tagtagttat agctacattt gttacaatag taggtatgct
     6301 tttaaaagca tatgttcctg ctcttgataa ggctcttggt atatatatac cattaatagt
     6361 tgttaactgt ttaatattag ctcgtgcaga aagttttgct ttcaaaacag gagctatgcc
     6421 ttcaatagtt gatggtgtag gtcaaggtct tggatttact gttgcactta caataatagg
     6481 tgcagtaaga gaattacttg gaaatggtag tttatttgga atgacattat ttggagcatc
     6541 tttccaacca gtattaatat ttatattacc accaggtgca ttcttaacat taggattctt
     6601 atttgcagga tttaataaat taagaagtaa aaaagcatag tggaggtgta ataaatgaat
     6661 ttaatacttt tatttttaag tatcgttctt gttaataacg ttataacatc tcaattcttg
     6721 ggtatatgtc ctttcttggg agtttctaag aaggttgata cagcagtagg gatgggagtt
     6781 gcggttacgt tcgttttaac tctagcttca attataactt attttataca gattttatta
     6841 gtaaaaactg gaactggatt cttacaaaca atagctttta tattggttat agcttctata
     6901 gttcagttcg ttgaaatggt aattcaaaag atgagtccat ctttatatca agcattagga
     6961 gtttatttac ctcttataac tacaaactgt gcagttcttg gtatagctct agttaatgtt
     7021 caaaaaagtt ataatttagt agaaacaata ataaatggat ttggagccgg agctggattt
     7081 actttagcaa tagtaatatt cgcaggtata agagaaagac tagagttagc tgatatacca
     7141 gaagcattta aaggtttccc tataacgctt atatcagcag gtttaatgtc aatagctttc
     7201 ttaggattta caggacttat aaagctataa aaaagagggg tgaaaatggt gatacttaca
     7261 gctgtattag ttttaggaat tatgggctta atcttcggga tagtactaga tttcgcatct
     7321 aaaaagtttg ctgtagaagt agacgagaga gtggaagcta tactaggagt acttccagga
     7381 gccaactgtg gaggttgtgg atttccaggt tgtggaggtc ttgcaaatgc gatagttgaa
     7441 ggcaatgcac cagttaatgg ttgccctgta ggtggagcag atgttggtgc aaaagttgga
     7501 gaaataatgg gaataagtgc tgaagcaggt gagaaacaag tagcaaaagt tatttgtaaa
     7561 ggaacttgtt caagtgcaaa agataaatat gagtatgaag gaatatcaga ttgtagagca
     7621 gcaaatgttt taaactcagg tgctaagatg tgtaaatttg gatgtttagg tcttggaaca
     7681 tgtaaggatg cttgtaaatt tgatgctatt tctatagttg atggaatagc agtaatagat
     7741 gaagaaaagt gtgtaaattg tggaaaatgt aaagaagttt gtcctaaggg tataataatc
     7801 acaaaaccag aaagtcaaga agtagttgta gagtgtaata gcaaagagtt tggtaaagct
     7861 gttaaggaaa aatgtactgc tggatgtatt ggctgtggaa tgtgtgtaaa agcttgtaaa
     7921 tttgatgcaa taatatttga agacaaaata gctaagatag accctaataa gtgtgtaggg
     7981 tgtatgcaat gtgtagcaaa atgccctaca aaagtaatat caggtgacat aactaagaaa
     8041 aagaaagtta ctattgacca agaactttgt gttggatgta cagtgtgcaa aaaacaatgt
     8101 aagtttgatg caatagaggg agaattaaaa gaaaaacata aggtagatgc agataaatgt
     8161 gtaggatgcc atttatgtat ggaaaaatgt ccaaagaaag ctattaaaat tttgtaatat
     8221 ggacaaaata aggatatgga ataatttcca tatctctaat gagtacataa aacagaggta
     8281 tggaataatt tccatacctc tattttatgt atatttattc taatggtaat aaatataaca
     8341 atagtgtaac aaaaaaggtt cgacatttct gttgaatttt ttacaatata gtgatagact
     8401 aaataggtac aaattacgta caaatgaggt gaataaatga atataatctt ggcatcagcc
     8461 tctccaagaa ggaaagaaat tctagagaat acgaatgtaa gatttgatat aattaaaaat
     8521 gaaattgatg agattatatt ggaaggtgaa acaccaaaac atttagttat gagattagct
     8581 tttgaaaaaa gtatgtctgt agcatctgaa cataatgaag atgttgttat tggagctgat
     8641 acagttgttg ttttagacaa tgctatttta ggaaaaccaa aagatgagtc ttgtgcaaga
     8701 gatatgttaa aaagactatc tggaagagag catcaagtca taacaggtat tagcttaata
     8761 aacttgtgcg aagataaaaa ggtaattgac tatgtaatca gcaatgtgaa atttaaaacg
     8821 ttatcagagc aggatattga ggattatctg aaaactaatg agtcatttga caaagcagga
     8881 gcctatggta tacagggtta tggagctttg ttagttgaag aaattagagg agattatttt
     8941 aatatagttg gtcttccaat ttcaaggtta ggcgacctat taaaaaaata ttttagtata
     9001 aatctttttt atggagtgtg atttatctga aaaagtcatt taattcaacc attaaggtaa
     9061 aagaaatggc accagaggaa agacccagag aaaagatgct tgccaaaggc gtaaaaagct
     9121 tatcaaatgc tgagttgttg gctatacttt taagaactgg caacaagaat aaaaatgcta
     9181 ttgaattagc caactatata ataaatagag atattcaggg tattagacat ttagaagata
     9241 tgacaataga ggagctgtgt aatatagatg gaattgggtt atcaaaatca acccaaatta
     9301 aagcagcttt agaactaggc tctagagtag ctagttttaa acctataaag tataaaataa
     9361 ggaatccttg ggatatacag aggtattata tggatagcct aaggtattta aaaaaagagg
     9421 tttttaaagc agttctttta aacacaaaaa atgagataat atctgatgta gatgtatcta
     9481 tagggacttt aagctcgtca ttagtccatc caagagaggt ttttaaagaa gctataagaa
     9541 gaagtgcaag taagataata gtgatgcata atcacccctc aggaagtgta gaaccatcaa
     9601 gagaagataa aaatatcaca tcaagactta ttaaatgtgg agaaataatt ggaatagaaa
     9661 taatagacca tataattatt ggagatggat tatactttag ctttaaagaa aatatgataa
     9721 tttgacttta tatgtgataa aataatagga gaatgtttta aacatgattc ttaatagtag
     9781 gaaggagtaa aatcatggct aaggaaaaaa agaaagaaaa aaaaggtttt tttagtttta
     9841 ataagatgac aaaggatatg ggaatagatt tagggactgc aaatacatta gtttacataa
     9901 aaggacaagg tattgttgtt agagaaccat cagttgtcgc aataagagat gatagtaaag
     9961 aggtattagc agtaggtgaa gaagccaaaa aaatgatagg tagaacacca ggaaatatag
    10021 tagctataag acctatgaaa gatggagtaa tagctgattt tgacataact caatcaatga
    10081 ttagctattt tatacaaaaa gcggctgata aaaaaggtgt tgtaagtcca agaatagcta
    10141 tatgtgttcc ttttggagtt acagaagtag aaaagagagc tatagaagag gctgcaagac
    10201 aagctggagc taaagatgca ttccttatag aagagccaat ggcagcggct ataggggctg
    10261 gattaaaagt tgaagaacca gaaggtaata tggtagttga tattggtgga ggtacttcag
    10321 aaatagctgt aatatcctta ggaggaatcg ttactgctaa atctataaga ataggtggag
    10381 acgaatttga cgagtctata gtggcttatg taaaaaaaga atataatctt atgataggtg
    10441 aaagaactgc tgaaaatgta aaaattaaca taggttctac atttaaagat gatgaagaaa
    10501 taaatatgca aataagaggt agagatttaa tctcaggatt acctaaaaca atagaaatat
    10561 gctctacaga agttagagaa gcattaaaag aacctgtaag ttcaatagta gatgcaataa
    10621 aatctacact agaaagaact ccaccagaat tagcctcaga tataatggaa aacggaataa
    10681 tgctaacagg aggaggcgct ttgcttagag gtcttgataa gcttattaca caggaaactg
    10741 gtatgtctgt acaaatagca gaaacacctc ttgattgtgt tgcacttgga acaggtaaat
    10801 ctgttgaaga ccaagaaatc tttgaaaaag tattaatgat gaatactaag aattagaagt
    10861 tggtgatggc cttgagattt gacaagaaca aaaatgagaa aaaaaggatt aatgttaaag
    10921 tgatagcaac gggcgtagtt gctatcactt taattggaat tgtgggtata tcaattggta
    10981 aattttctag tggtagtcca gtaaatttag gagtagcttc agatgctatt acaagtgtag
    11041 gtaagggaat aaatgatgga ttttctttta tcaaaaatgg ttttaaagat gtagcaaatt
    11101 ttaaagataa ttctaaaaag gtaaaaaaat tggaagaaga aaatgaaaaa cttaaaaaag
    11161 atatgatagc tttaaatgcc aaactagata aaacagaatc gctagaagag ttaaaaaaga
    11221 cactgaactt tgtacaagaa gagtataagg cgacaagtat ttcagcaagt gttgttggta
    11281 aaaatgatgg aaactggtat gagagttttg tcataggtgc aggaaaaaat tcaggtgtaa
    11341 aaaaagaaag tatagttatg aatggtagtg ggttagttgg catagtttat gaagtttcca
    11401 acaattatag taaggcaatt tcactattgg attctaaggc atctgtaagc tttaaactag
    11461 caaaggatgc caatgctaaa ggtacaatta ctcaaaatac tactttagat aataaagata
    11521 gttataatag taaaggatat ttgcaaggat atatgtttga ctcatcatat aatgtaattc
    11581 aaggagatat aataacaaca tcaggtcttg gattcttccc agatggtata ccaattggcg
    11641 aagttgaaaa ggtggttgat gataaagata agtcattaaa atatgtagtt gtaaaacctt
    11701 atgttgattt taagaatata aatgacgttg tagtcataga accaagaaat ataggataaa
    11761 ggggataaaa acaatgaaaa aagttttact ttgtctgttg gggattgtgc ttatgactat
    11821 agaaaatagt ataacaaatt atatagatat ttttggcata agctttaatt tagttcttat
    11881 atatgtaacc ataatctctc tttatttaga tgaattagaa ggtgggatta tagcagcaat
    11941 aatagggctt attaaagaca taaccgttgg tggaattttt ggtgtaaatg cattaatttt
    12001 gtttattatt gcatatgcaa ttggatacat gagagatcag ctatataaag gtagttatat
    12061 tattactttc gttttaattt ttataggcag tttaattgat tcaattgtca atataggtac
    12121 atctagtata atttatcaaa gctacaacat atcaacactt tttgtaaaag gtctactagt
    12181 agcaccaata gtgaatagtt taattggttt agtaatatat cgcttatcta aaagagcagt
    12241 tttaaaactt aaagaagatt agttggtgat ttcatggaag aaaataaaca agttgataga
    12301 ctaaaaataa ttaagatagt catgagcgtg atatttttag ctattttagt aaaaataatc
    12361 tatatgacta catttaaata tgaatattac aatgagttag ctgagaataa aacatataaa
    12421 aagttggcaa tagaagctcc aagaggagaa ataaaagata gatatggaag actgttggca
    12481 ggaaataaaa acttatttac tgttcaagtt tcaggtaacg atattaataa gaaagatgca
    12541 aataagcatt caagagcaaa tgaaatatca ttaaaactta taaatttgct ggagagaaat
    12601 ggtgaagaat atgtagatga gtttcctata tatgtagaaa atggaaagta ctattataca
    12661 tatgacagag atattaggga atataaatca gaaaatggta ttccaaatga ttataatgct
    12721 aaggaaagtt tttattactt agtagacaaa cttatatcag ctggaatatt atctcaagaa
    12781 gataaaagat tggatgcgac aagattacag gcaaaactaa atgaaaatgg atattatcca
    12841 cctattttag ttagtaaatg gatgttcaca gcagaaagag ataagagaga ttggttggct
    12901 agttataaga taaaagaaac taaactgagt gccaaagaag cgtttgaaaa agtaagaaat
    12961 agtgatgcat tagaaattga taaaagtttg agtgatgaag atgcaagaaa aataatggtc
    13021 gtaagggatt tgataaaatc aaaaggatat tctcaatata atccagtaac tattgctaag
    13081 gatgtaggag aaactacgat tgctcaaatt gaagaaagtg ctatggacct tgttggtgtt
    13141 tcaatagcag tagaaccagt aagatattat cctaatgggt cattagcttc tcatatgtta
    13201 ggatatgtag gaaaaatgcc atccactcaa atagaatcat accttcaaaa aggttatgaa
    13261 actggagata tggtaggtct tgcaggagta gaaaagagta atgaaagtag attaagagga
    13321 actgatggtt ataaaatggt taaagttgat gcattgggta gaatcagtaa agaaattgaa
    13381 tctaaaaaac ctaaatcagg agatactgtg tatcttacac tagataaaga tttacaagaa
    13441 gtttcagata atgcattaaa acagataatc gaagttgctt ctaaaggagg tacttttaaa
    13501 agtaaatttg gggacaaacc aattagtgca tatgcaggaa aagcacagtc agcagctctt
    13561 atagctattg atgttaaaaa tggagaagta ttagcttcat caagttatcc taattatgac
    13621 ccaaataaat ttgcaaaagg tatttctaca gaggattata aagcacttca accaaaaaat
    13681 ccaaatgact tactagcagg aagtccactt ttaaacttgg ttacacaagg ggaatttcag
    13741 ccaggttctt catttaagat ggtaacttca atggcagcat tagaaaatgg tttggatcca
    13801 aactttacta taaatgaccc tggggttata atgttaggta aaaaatcttt tggtgactat
    13861 gtatggaacc atggtagagg aaaccatggt atgacaaact tatataaagc gatacaagag
    13921 tcttgtaata tatatatggc aacaattggt acaggtaaaa catggccaga tggaaaaagc
    13981 attggtatag atatgaatgc aaataaaata cttgagtatg caaaattatt tgggcttgac
    14041 caaaatacag ggcttcaaga tgaagtagaa gaaagagcag gtaaagttcc tagcacagaa
    14101 gataaattga aatcaacaca agctcttcta aagtcaaatt tagaaagaga aatggcaaat
    14161 gattttgttg atataactag agaaaaaaat cctaaagaat atgagaaaag aatcaatgag
    14221 attgtaagct gggcagcaga aaagaaaact cctggtagag tcgaaacaat gaataggcta
    14281 aagaaaatga atgttaaaga agatagaata gaagaagtag cagatttagc agtattcagt
    14341 tattttaact ttgcaaaatg gagtactgca gatacattta acttagctat aggtcaaggt
    14401 gaaaatgctt atacaccagc tcaaatatca agatatgtag ctgctatagt aaatggaggt
    14461 aatttagtag aattatctgt agttgataga gctgtttcaa gtgattattc atcagttaaa
    14521 attaatgacc agaagaaagt cgaaaaaata ccttttaaaa atccagataa cttaaaagaa
    14581 ttaacaaaag gtatgaaatt ggttgcaaga caaggtacag cgaagagtgc ttttgctgat
    14641 tttccaatag atgtagcagc aaaaactggt acagctgaga aaagtgggaa aatacctaca
    14701 gataacgagt atgaatattt aaaaagtcat atgtcatctt ataatgtcaa cctaaatgat
    14761 gcgattaaac tagctgataa gatgaaagca gaaaaagaaa aagagctttc attagctaag
    14821 gaaaaggaaa taaaaaagaa attagaaaat aaagatttga aagacgaaga aagaaagaaa
    14881 ttagaggaag aattagaaga tggagtaaaa gttagattag aggacactga taaggtgaat
    14941 tcttcatatc taagaaaagc tataaaagaa ttaaatccaa aaataacaga tgaccaaata
    15001 gatagattta aacaagatta tggttctttt acatggactg ttgcttttgc tccagcagat
    15061 gacccagaaa tagctgttgt atgtgtaatt cctcaaggtg attcaagtgt attctcactt
    15121 ttaccaacta gagaagttat tggaacttat atgggattag agccaactaa tagtaaaaat
    15181 gacaacaaga cgaatgatgt taacaattct tctgacgaaa atataaattt cgagtcacaa
    15241 attaatagat agcttaatct atgtataaac tttagcttta tatagaaaaa ataaaagggt
    15301 ttaaagcgta tttgaagaat ataaatgata tatcactctg gaggttgtta tgtctttaag
    15361 agaaatttgt agtcaagaac tagtagagtt caagggtaat aaaagaggga taatagtaaa
    15421 tataaaaaga gaagctccat ttgaggaaat acaggaaaaa ataataaaca aattggaagc
    15481 atatgttggc ttttttaatg gggctaaaat tagtaaaatt aatagtgatt gtttaactga
    15541 catggagatt ctagaactta aagaaggaat tacttctaga tttgatgtgg aatttgttga
    15601 agatcaaaaa attgaagaaa atagtaattt tccaacaaaa tatgtcaata ccctaagatc
    15661 tggtgaaaat attgaatttg aaggtgatgt agttatattg aatgatatga aacctggttc
    15721 aaaagtcctt tcaaaatcaa atacagttgt tatgggagat ataaatgctg gtgctaaggt
    15781 tgtagcagga ggtaatgttt ttgtaatggg taaaatagaa ggttttgtac atgcaggtgc
    15841 agaaggcaat gaatttgcat atgttgttgc tggaaatctt aatcctaaaa tattacaaat
    15901 agcagataat attgcagaag ctccagatga tgaagaaaat tatgaaaatg aatcagaaat
    15961 tagcccagaa atagcctttg taagtaatgg aagaattgta atcgaaagct atttgtcaaa
    16021 gttagacaaa tagaaagaaa aatagggggt agtattatga gcgaagttat agttataaca
    16081 tctggtaaag gtggggttgg aaaaactact accgcagcaa acttaggaac tgcgctaagc
    16141 ctagaaaata aaaaaacagt agttgtggat gcagatattg gacttagaaa cttagatgta
    16201 gtaatgggtc ttgaaaatcg aatagtttat gacatagtag atgtagttga aggaacttgt
    16261 aggcttaagc aagctctaat aaaagacaaa agatttgata atctatattt attaccagca
    16321 gcacaaacta gagataaaaa tgctgtctca gttgagcaga tgattgatct gtgtgagaaa
    16381 ctaaaagaat ctttcgagta tataataata gactgtccag caggtattga gcaaggcttt
    16441 aaaaatgcag tagctggggc agatagagct attgtagtta ctaatccaga aatatcagca
    16501 gtaagagatg ctgatagaat aataggtcta ttagaagcaa atgaaataaa agagataaga
    16561 ttagttataa atagaattag aaatgatatg gttaagcgtg gagacatgat ggataaacaa
    16621 gatataatag aaatattagc aatagatttg ttaggtcttg ttcctgatga tgaaagcata
    16681 attatatcaa caaataaagg agagccagct atacttgatt ctaagtcact tgctggtcaa
    16741 gcatacaaaa atatcgcaaa aaggatacta aatgaagaag ttcctctact tgatcttgaa
    16801 gttgaagatg gattctttgg tagacttaaa aaaatgttta gcatggctaa gtagcaaagg
    16861 aggatacatc tgtgttagat ttatttagag ttttttctaa cgaggctaaa actagtaaat
    16921 ctgttgctaa agagaggtta aagctagttt tagttcatga tagagtagat tgttcaccac
    16981 aacttttaga gatgataaaa acagacattt taaaagtaat agctaattat gcagaaatag
    17041 aagatgatgg tcttgaaata aaaatgtcta aatgcagagg tgagcatgat gacaaacctg
    17101 tatctgcatt agttgctaat ataccactaa agaatataaa agatagatgt atgtaattag
    17161 tgcttaaaag ggggatatcc ttattggtat atgccccttt tatttaatta tatgtttaaa
    17221 gatagaaaga cggtgattct ttgaaaaaaa tcaatattaa ttataagtcg acaattaagt
    17281 tgataaaaca gttagactgg aaacttatag ttacagtatt agctatcttt atttttgggc
    17341 ttgttatact tagtagtgca acacatgcta attcaacagg ttcatataat caattgatta
    17401 aacaaggatt agcttttgta cttggtatag gaatgattat agtcatatta ttttttgact
    17461 ataatcttct aggcaggtat tataaagcgc tttatataat aagtcttatt ttgttagcca
    17521 tagttttatt acctggaatt gggacagtaa aaggtggagc taggtcgtgg attaacttag
    17581 gaccacttga cttgcagaca tctgaaatag tcaaattaac atttgtacta agttatgcaa
    17641 agatacttga atccaaaaag gataaattaa atacgttaaa ggaagttatg cctgttgtag
    17701 tttattctct tccatttatc ggattattga ttgcacaacc agacttggga acaggaattg
    17761 tattttgttg tatgatattt gcaatgctgt tcacagcagg tcttagctca aagttgataa
    17821 aaagaggaat aatcatacta ttagtgtcaa tgccactgat gtatttaatg atggcagacc
    17881 atcaaaaggt aagaatagaa gcttttctaa atccagaaga tgtaactcta aaaggaaact
    17941 atcaagttat gcaatctctg attgcaattg gttctggggg cgtaactggt aaaggacttt
    18001 acaatggaag tcaaaatcaa gaagattttt tacctgtaca agatagtgat tttatatttg
    18061 ctgttgtagg tgaagaactt ggtgtaatag gaatggcagt gttgataatc ttatttatga
    18121 tattcctatt aaggctttta gcaattgcaa gagatgcaaa agatttttat ggaaccctca
    18181 tagtggttgg tgttatggga atgtttggtt accagataat ccaaaatata ggtatgacgg
    18241 ttgcgttaat accagtaact ggagtaacat taccttttgt aagttatgga ggaagttcgt
    18301 tattgacatc tttagcaaac ttaggattag tactaaatgt gtgtatgaga agaaagaaaa
    18361 taaatttcta aatttaaata catctaaaca taaattgagg agattagata tatgaatata
    18421 gcattagtag cacatgacca aatgaaaaac actatggtcg gattttgtat aggttatgaa
    18481 tcaattttaa aaaagtatgg actatatgca actggaacta caggaaaaag aataatggat
    18541 gaaactgaat taaatataaa tagattagca tctggtcctt taggtggaga ccaacaaata
    18601 ggttctttaa tagtaacaca agaaatagat ttagttattt ttttaagaga cccattgaca
    18661 tctcaggctc atgaaacaga tatacaagcg ttaataagac tttgtgatgt ttatcatgtt
    18721 ccaatagcta caaatctagc ttcagcagaa atatttataa aagctcttga tagaggagaa
    18781 ctatcatgga gagaagttag aaaaagcaaa agtcaacgta tttaaaaaat attaatatta
    18841 cagtctaaag aaacagatat ataagtgttt aatgccctag tttttctgtt gcaataaacc
    18901 tagtacctgt agaaatctta tgaaagattt aaatgatgag gagctaaatt tgagattaaa
    18961 taattaggaa atagattata gatagagtag tagaatttaa actattactc tattttaata
    19021 atatgaaata gaat
//
LOCUS       DAEPLF010000056        18856 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEPLF010000056 DAEPLF010000000
VERSION     DAEPLF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.056"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            176..2395
                     /locus_tag="KR314_001702"
     CDS             176..2395
                     /locus_tag="KR314_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0328089.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYALNQDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKITISVPLLNNDK
                     VVGVLYCTYNINTLMKLINVSFYENNSISYVVRNDGTIILHPQEDSLSKNIYKLLKQD
                     NDIREINRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSEQIINRAIYAVLFIILIFIAIIFYIIYIKKSNEKEILSLAYEDKVTHIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGKNKRIRKIDSIRNNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LSEWKKQGKKIVPVSINLSRVELYQPDIVKFINKTIQMYNLSSDLIEIEITETVAINE
                     LNILKNVLNELRKYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLNGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            2649..3311
                     /locus_tag="KR314_001703"
     CDS             2649..3311
                     /locus_tag="KR314_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328090.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            3681..5090
                     /locus_tag="KR314_001704"
     CDS             3681..5090
                     /locus_tag="KR314_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBH0328091.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     SLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGGIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            5205..5480
                     /locus_tag="KR314_001705"
     CDS             5205..5480
                     /locus_tag="KR314_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBH0328092.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DITVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            6110..6421
                     /locus_tag="KR314_001706"
     CDS             6110..6421
                     /locus_tag="KR314_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328093.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VRKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            6423..8348
                     /locus_tag="KR314_001707"
     CDS             6423..8348
                     /locus_tag="KR314_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBH0328094.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            8351..8842
                     /locus_tag="KR314_001708"
     CDS             8351..8842
                     /locus_tag="KR314_001708"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBH0328095.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            8894..9457
                     /locus_tag="KR314_001709"
     CDS             8894..9457
                     /locus_tag="KR314_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBH0328096.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            9472..10449
                     /locus_tag="KR314_001710"
     CDS             9472..10449
                     /locus_tag="KR314_001710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBH0328097.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            10436..10759
                     /locus_tag="KR314_001711"
     CDS             10436..10759
                     /locus_tag="KR314_001711"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBH0328098.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            10876..12654
                     /locus_tag="KR314_001712"
     CDS             10876..12654
                     /locus_tag="KR314_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBH0328099.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            12651..14024
                     /locus_tag="KR314_001713"
     CDS             12651..14024
                     /locus_tag="KR314_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBH0328100.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            14030..14698
                     /locus_tag="KR314_001714"
     CDS             14030..14698
                     /locus_tag="KR314_001714"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBH0328101.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            14975..16024
                     /locus_tag="KR314_001715"
     CDS             14975..16024
                     /locus_tag="KR314_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0328102.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTYNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            16120..17109
                     /locus_tag="KR314_001716"
     CDS             16120..17109
                     /locus_tag="KR314_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0328103.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            17114..18832
                     /locus_tag="KR314_001717"
     CDS             17114..18832
                     /locus_tag="KR314_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBH0328104.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
BASE COUNT         7311 a         2202 c         3478 g         5865 t
ORIGIN      
        1 taatttaaaa ttcaactaaa tagtgttatt atgataataa aagaatgttt ttatggttat
       61 taggttgatt ttatgtgtaa catttaagaa tagttatata caatataaat gatgaatcgg
      121 tataaaaagg atatattatc tatatgaagt attgactaat taggggagga gtcgaatgac
      181 agggagaaat aaaagtttta cattaattac aattcttttt ttaacatcat tatttttgac
      241 aatggggtat atctatatag aagatacaaa acatcttctt atgtcagagg caaaaataca
      301 tataaaagaa gttgcagtac aaggctctca actagcacaa agacaaattg aaaaagattt
      361 agatgtttta aatatttttt ctaactatta tgctttaaat caagatatac ctaatgagga
      421 aaaaatgaaa aatcttttag atgaaatgga aaatcaaaaa ttttacacta tggcaatagt
      481 tgatataaat ggtaatgctg aaaataccaa aggtgataag ttttcagtga aagatagaga
      541 atttttcaaa aactcaatta aaggtaagaa gtatgtttca agtccatatg ttgatgaagt
      601 aaataagtct ataaagaaga ttactatttc agtaccatta ttaaataatg ataaagtagt
      661 tggagtttta tattgtactt acaatatcaa tactctgatg aaacttatta atgtatcttt
      721 ttatgaaaat aatagtatat cttatgttgt aagaaatgat ggtacaatta tccttcatcc
      781 gcaagaggat agcctatcaa aaaatattta taaattgtta aaacaggata atgatattcg
      841 agaaataaat agattaaaaa aagaattgca agaaaataaa acaggtgcaa ctgtattaaa
      901 tatgttggaa gaaagaagat atttaggata tgctactatg gataatggga atagtgaaaa
      961 taattatata aaagattgga atttgatttt ttcaattcca gaaactgttg tatttagtaa
     1021 ttcagaacaa ataatcaaca gagctatata tgcagtgtta tttattatat taatatttat
     1081 tgcaattatt ttttatataa tttatataaa aaaatccaat gaaaaagaaa tattaagttt
     1141 ggcttatgaa gataaagtta cccatatagg aaatcaaaat aaattttata gagagtgtag
     1201 taagtatctt ttagataaac catctttaaa ttatattata gtatattttg atattaataa
     1261 ctttaagatg attaatgata cttttggata tgaatttggt gacaatttat taattacgat
     1321 agcaaaagct ttaaaagaag aattaacaga aggtgaagta tatgcacgac tttcaagtga
     1381 ttattttgca attttctgtg attataaaaa tggaaaaaat aaacgtataa gaaaaattga
     1441 tagtatacga aataatattg aaagtaattt gagtatagta tttgaaattt ctctgtgtgt
     1501 gggtatttat tttgttgaag aaggtgaagt agatatccaa aaggctgtaa ataaagcaaa
     1561 tatggctagg tctgttgcta aaggtaaaaa tataaattat gctatttata atgaagatgt
     1621 taggaataag cttagtgaag aatcaatgat tttagatgat ataaaaatag cattggtaaa
     1681 aaatcaattt gaagtgtatt atcaacctaa gttttcactt gtaactggag aaatgatagg
     1741 gagtgaagca ctaataagat ggaatcatcc agaacatggt tttataagtc ccgcagtatt
     1801 tattccaata gcagagaaaa gtaagttgat tttaaaaatt gggagatttg tatttgaaag
     1861 ggtttgcaat gatttatctg aatggaaaaa gcaaggtaaa aagatagttc cagtttcaat
     1921 aaatttatct agagttgagt tgtatcaacc agatattgta aaatttatca ataaaacaat
     1981 acaaatgtat aatctaagtt cagatttaat tgaaatagag ataacagaaa ctgtggctat
     2041 taatgagtta aatattttaa agaatgtttt aaatgagttg aggaagtatg gattttctat
     2101 ttctatggat gactttggta caggatattc ttctattagt tgtttaagag atatgccaat
     2161 tgatatattg aaactagata agtcttttct taatggtatt gaacatgatg agagaagtcg
     2221 caatatagca aaatcaatag tgtctctagc aaaatcacta gatttagttg taattataga
     2281 aggtgttgag agtaaagaac aagctgagtt aatgaaacaa tttggatgtg atttagttca
     2341 aggattttat tttgcacgac caatgccagc taagaatttt ttagatttac tttaaaacaa
     2401 aatttaattt ttatataaag cactattatg aatgtttgat tttaataaat cactgctaaa
     2461 taattagtgt ttttttattt tatcaatgta ttgtttaata taaagcaaaa aataattgta
     2521 ttttataaaa attatattaa aaataaattt attttgctaa taaacatgtt attaacaaaa
     2581 gaatagtact ataatgtata gatgataaaa actttaataa aaatttagtg tataggagtt
     2641 gtttaagtat gatagatgtg agtattgatg acaaacttaa ggaaagatgg cctaatgcta
     2701 agttgggttg cattcaagca aaagtagcag taaataaaag ctcagaaaaa ttaattaatg
     2761 aaataaatga gttttgtgat actataaatc aaagtctaaa agttgaagac attacgaaac
     2821 tagataaaat taaagatgca agaaatgctt ataaagagct tggaaaaaat ccaagtagat
     2881 atagaacatc ttcagaagca ttggttagaa gaattattca agggaagggt ctttatacaa
     2941 taaataatat agtagaaata aacaatttaa tatcaataaa atcattatat ccagtaggta
     3001 catataatgt aagcaaatta cattcaccag tatgttttac tgtaggagat gaaggagaac
     3061 agtataaggg tattgggaaa gaattaataa atatagaaaa tcttcctata ttgtctgatt
     3121 cagtgggtaa atttgggagt ccaactagtg attctgaaag agcaatgata acagaagatg
     3181 caaatgaaat attaatttgt atattttctt ttagtcaaga atctgatatt gaagaatatt
     3241 ttggatatgc taaggagtta cttaaagaat atgctgatgg aaaagatata gaaactaaaa
     3301 taattaaata gaatctatat aacaacgtta tgttaagcta aaattcaata taaaaataag
     3361 ttaagataaa gaaactaaca taaaattagt ttctttttta aaagagtagt ttagtgttac
     3421 aaaaacagat agataaccca cgattattaa aagtatggta atatattaat atagcagaga
     3481 ttgtatttaa gggattttga aaggggtgat ttataatagt cattttgtct aaaagataaa
     3541 atggttttat ggaaataact atatatttaa aaatataaac aatgatacag taatttgggt
     3601 atataaaata aggcatacat ggttttgttt aaaaaccaat aaagtacata ggtaattaca
     3661 atgtaaggaa ggatttaatt atgattgatg gaaaagaggt aaaacaagaa agtggacaaa
     3721 aaaatctaaa atttaagata gctatagttg taggtgtttt gtttgtagct tatataggaa
     3781 tggcaatctt ttttagaaat catttttact ttggaacgac tattaatggc gtaaaggctt
     3841 caggaaaaac agttgagcaa gtaaatgatt tgttaacatc tagtactaat tcctatactt
     3901 taaacttaga ggaaagaaac aataagaaag aacaaataaa agcaaaagat attgatttaa
     3961 aatttacacc agatgataaa gttcaaaaga taaaagatag tcaaagcgct attggatgga
     4021 ttggtggaat atttaaatct aaagagtata ataatatgat aactctttca tataataaag
     4081 atttactaga aaaaaggttt aatgctttat cttgttttga tagtaaaaat gttgtagaac
     4141 ctaagagtgc ttatcctaag tatgatagca ctaaaaacgc ttatgttata gtaccagagg
     4201 tacttggaaa caaagtgaaa aaagatgatt tttataagtt agtacaaaac tcaattaatt
     4261 ctgggaatac ttctatttct ctggatgaaa gtaaagttta tgaagcacca aacaacacaa
     4321 aggattcaga aaagttgaaa caagctatta agacattaga taagtatgtt ggtgtagtcg
     4381 ttacatatga ttttagtgac cgtaaggaaa ctttagatgg ttctgaaatt aataaatggt
     4441 taaaagtaga ctcagaaaag aattatgaca taacttttga taatgatgct atggtagaat
     4501 atacaagaag tttaagtaaa aaatacagta cttttggtga ctcaagacca tttaaaactg
     4561 ccacaggtaa agatattaca atttcaggtg gaatttatgg ttggttgata gataaaaaga
     4621 aagaagctga agcattagtt gatgttgtaa aagctggtaa aaatgttaca agagaaccta
     4681 tttacataca aaaagctgta tctcgtaata aagatgatat tggaaataca tatgtagaga
     4741 ttagtctttc tggccaacat atgtggttct ttaaaaatgg agaaatgctt gtatcaacag
     4801 atgttgtaac aggcgattta gctagaggat ttgctacacc atcaggtata tatcctctta
     4861 attataaggc tagaaatgtt tcattaactg gtcaaggtta cagttcacct gtaagttatt
     4921 ggcttccatt taatgggaac ataggcattc atgatgctac ttggagaaat tcttttggtg
     4981 gaggtatata taagtcttca ggttcacatg gatgtgtaaa tacacctttt tctaaggcaa
     5041 agaaaatata tgaaaatata gaaccaggta caccaattat tttatactag taaatgtgtt
     5101 gctaacagtt aagcgaagtc atcttagttt atttaggatg gcttcctttt tatataaagc
     5161 ttattgaagt ttacagaaag actacttaaa aggagaaata cataatgaat gaaaaattaa
     5221 ctaaaatttt agaagaagct aagaatgatg atgaatttag aaaggaactt ataaaaacaa
     5281 gagaacaacg tgatgttatg gattcatttt gtaaggttgt tacatctaga ggttatgata
     5341 taacagtagg ggaacttttt tccatgggag aagaatatac atcaaaccta ctgaaaagtg
     5401 taaatggagg agcagcatat cctattgaag attggagtga ttggtacgag gattttttta
     5461 taatgttgtc gaatatatag agtaactaat gatattatct acttaagatt aatacattgc
     5521 cttaaaataa aactattaga actaagtgta agttttatca atctttaaaa atattgattt
     5581 atttctaact atatataaaa aatttagatt gaaaataagg tgacaaaaat cgatatagct
     5641 tgatgattta taaaagttag ataaaatagt gcgagatatt tctaaaaatt caaaaaaaac
     5701 aataaacttc aagggttaga ggttgaaaaa gaatataaaa aacttttata tgtaataaat
     5761 taaattaaaa ttataaaaaa aataaaataa aaatttaaaa tgtcgacata gcgttaattt
     5821 taaaacaaaa taacgctatt gacaaactat atcgctttaa attataatta ttgaaaataa
     5881 tggtatttaa aaaccatact catgtcatag tctaaaagta agtcaaaaaa ggatgtaaat
     5941 ctaggcaagt actattctaa aatatgtttt attaagaaat aaacgttgaa aaatattaat
     6001 tattatataa aaatgctgtt tatactttaa caatgaggta gtaatctaat tagaataaaa
     6061 aaatctaatt ggttatatac atagtaaaaa ggtaagagag gtgatttgga tggcttttga
     6121 aataataaaa agcattgttg aggcagagca gacagcagac agtatcaaag taaaagctgt
     6181 tactgatgca gagtctatca gagctgatgc tgtaaacaaa tgtgaaagca tatttgctga
     6241 tgtaagaaaa caagcaaagc ttatggaaga aactcttatt gagaaggcag tcaccgacag
     6301 tagagcagag gttgataaaa tcttagctaa tgctaaaagt gaatgtctga aaattgaaaa
     6361 aactgctgaa gaaagaaaaa gtaaggctat tgaagcggtt attggaaagg tagtgagata
     6421 atatggcaat agttaatatg aacaaaatat ctattgttgg cttagaagct caaaaaagcc
     6481 agattttaaa gctacttatg aaccgtgggt ttgttcagat tgatgatagt gcctttctta
     6541 ctgaggagga ggaattaaaa aattatcttg taaaagatag tgaagaatct gaagttataa
     6601 tgctagagca gaaaatgtct catgtaaacc aagcaattaa tattgtttct tcactagtga
     6661 aacaaaaaaa atccctactt gcaccaaaaa gagagtttaa caaaattgat agtgaaaaag
     6721 aagggcaagt ttatgctgaa gctctggagt taaacaattt gcacaaggag ataggtacaa
     6781 taaggtctaa cataaactta cttaacaaca ataaaagtat gttagaacca tgggtgaact
     6841 ttgatattcc tcttgaaaat atggaaacta agtatacaaa aactatactt ggaactgtgc
     6901 ccataagcgc aaatattgat gctttaagag taaaattaga agatgaaata ccagaaagtg
     6961 ttattgggat tgtcaattct gacaagttaa tgaactatgt ttatttaatt actttaaaag
     7021 aaacttttga tgatgtttta gaagttttaa aagaatttaa tttttcagtg gtttcattac
     7081 caaatgaaga aggtacaccg ttacaagcta tcaagaaata caatgaaatg ataattaggc
     7141 aggaaaacgt ttctaaggac aaaacagagg aaattaaaag acatgcagat agtatacaca
     7201 tgcttgaaaa cctatatgat ttttacacta tagaaagaga ccaaaaaaag attatagaga
     7261 gacttgtcaa aacaaaaaca acattctgtc ttaatggttg gttaccaagc aaaagggcag
     7321 atgaattaat taaagaaatt acagaaaagt ttgattgttg tgttcagact gaagaaggaa
     7381 gtaaagaaga aggcttccca attctgcttg aaaacaataa attagtaaca ccttttgaaa
     7441 gtgtaactaa catgtacagc tatccaagta caaaagatat tgacccaaat acaattttaa
     7501 ctttcttctt tgttgtattc tttggaatga tgctaagtga cgccgcatat gggttaataa
     7561 ttgcagtagt ctgtggattt gttgtatata aattaaagat acaaaaaggt gaaggaaatt
     7621 taattaaact tattggtatc tgtggagttt caactactgt atggggtctt atattcgggg
     7681 gtattttggg ggatttaatt cctataaaag cattaattaa tcctttagag gatgttatga
     7741 cacttatggg aatgtcgtta ctgttcggta ttatccatat atatgtaggt ttgggaataa
     7801 aagcatatac attaataaga agtggaaagg taatagatgc aatctttgac ataggttttt
     7861 ggtatttgtg tataacaggt gtatgtctac ttataatacc ttttgttgca ggggacatag
     7921 gtgtattttc agaagtagga aaatatttag caattatagg tgctataggt cttgtactta
     7981 ctcaaggaag aagctttaaa ggtattcctg ttaagctttt taaaggattt tcaagtctct
     8041 atggcataac aagttatttt gctgatatat tatcttatac aagaattatg gcgctttgtt
     8101 taacaacagg agttatagca caagttatca accttttagg ggctatagct ggtccgatac
     8161 ttgcagttgt aatcggtgtt gtaggacaca caataaacct tcttataaat gcactaggtg
     8221 catatgttca tacaagtagg ttacagtatg tagaattttt caataagttt tatgaaggcg
     8281 gaggagttcc ttttgttccg tttaagtata aaacaaaata tacaagcatt aataaaaagg
     8341 agatgtaaat atggaatttt taaacgcgtt aggaaatggt caatttttgg caatatcagg
     8401 agctgctata gcagcattat ttgcaggtat gggttctgct aaaggtactt caatagcagg
     8461 tcaagccgca gcaggtgttg taacagaaga ccctagtaaa ttcggtcaac ttctagtttt
     8521 acaacttctt cctggtacac aaggtctgta tggatttatc atagctttcc ttattttatc
     8581 aaaagcaggg attattggtg gagatgcaat accaacatca gctcaaggtt tacaacttct
     8641 tatggcagga cttccaattg gtttagttgg tttagtatca ggtatagcac aaggtaaagc
     8701 agcagcagca ggagttggaa tagttgcaaa aagacctgaa gaacttggta aagctataac
     8761 atttgcagca atagttgaaa catatgctct tttaggacta cttgtttctt tcttagctta
     8821 caatggaatt gcaattggtt aattataaat tataattcat aagtaaaaaa aatgaaaagg
     8881 aggaagcact cctatgggta atgaacaaaa aatgattgac agaattatag ctgatgccaa
     8941 acaagaagca caagaaatat tggataaagc taaaagtgaa gcagatttaa aagtaaattc
     9001 agctaatgaa aaagctgaaa aggaaatggc ttcctataca aagcttgcag aggcagaagc
     9061 agaaaaagcg gcttctaagg aaatatcagg agcttatatg gaggctaaaa agcaaatatt
     9121 atcaaaaaaa caagagattt tagaagaggt aattttagaa gctaaaaaca aacttttaaa
     9181 tcttaaagat aatgaatatg aagagattat actaaacatg attgaaaaat caaattgtac
     9241 agatgattca gagatagtat tatctaaaaa agataaaaaa acattaaaag atgtgctatc
     9301 aaaaaaaggc attaaagtgt ctgatgaaac tagagatatt actggaggtt tcatagttaa
     9361 aaaaggtgac attgagtaca attattcatt tgaagccata attgcagtag agcatgaata
     9421 tattgaacaa attgcagcag aaattttgtt taattaagga ggggttaaaa aatggcagaa
     9481 gaaaaaactt atccctatgc cgttgcacga ataagagtgc ttgaaaaaca gcttcttaac
     9541 agacaaatgt ttattcaaat ggcagaagcc aaaagccctg aagactcttt aagaattata
     9601 gctgaggcag gttatgctga tacatcaaat aataatatac ataattttga gaatattttg
     9661 actcaagaat tatcaaagac atatgaaatt cttaaaagtc tagctcctga agaaaaattt
     9721 gttgatgtat ttctttataa aaatgactat cacaatttaa aagtgctgat taaagaagaa
     9781 atttctggtg tagatggtga gaaatattta attgatggag gtacaattcc tcttattaag
     9841 cttagagaat ctttagcaaa tcgtagtttt agtgatttac ctaagataat gtcatcagct
     9901 atattagagg catttgatgt ttataataag acacaaaatg gccagatggt tgatattgtt
     9961 ctagataaag caacctttac cagtatgaaa gaaactgcta aagaaagcca aaacaaattt
    10021 gtaatagatt atgttatgaa agtttgcgat ttaacaaatc taaagagttt tataagagtc
    10081 aaaaatatga agaaagattt tgatatgttt atgaatgtgt ttgtttcagg tggctcactt
    10141 gataaggaaa aattcttaga agctttttcc tcagatactc cagcatcatg ttttaaatct
    10201 acttcttact ctgatgtctg taaaaatggc atggatagtg gttttactgt ctttgaaaaa
    10261 ctttgtgatg actatcttat ggaatatgtt agaggagcaa aatttaagcc tcttacatta
    10321 gaaccactaa ttgcatattt atatgctaaa gaatcagaga taaaaacaat tagaattatt
    10381 ttgactagta agcttaataa tatagacgca gatactataa aagaaaggct aagggatgct
    10441 tatgtataag gtaggagtag ttggagataa agatagcatc atgggatttt tagctcttgg
    10501 aatagatatc ttcccagcct atgatagtga cgaaattaaa aaaagtatac ataagttggt
    10561 tgaagatgaa tatgcgataa tttatattac agaacaggca tcattgcttg caaaagaatc
    10621 aatagcaaaa tacaaagatt accaacttcc tgccattata gttattccag gtattggagg
    10681 aagtatggga ttaggtatga atgaagtaag agaatctagt aaacgtgcta taggtgcaga
    10741 tatattattt aaagaataga tgaaagttga ataaaagaca atctcaaatt ttattggtgc
    10801 taaagatatg tgctaataaa atataggagg gaatatttaa attttgataa agtatatgat
    10861 tgcaggtgag agaatatgaa aacaggcaca attgtaaagg tgtcaggacc gcttgttgta
    10921 gcggagggca tgagagatgc gaatatgttt gacgtcgtaa gagtttcaga taaacatctt
    10981 ataggcgaga ttatagaaat gcatggagat aaagcttcta tacaggttta tgaggaaact
    11041 tccggtctag gaccagggga agaagtagtt tctcttggaa tgcctatgag tgttgaactt
    11101 ggaccaggtc ttatttcaac tatttatgat ggtattcaac gtccacttga aaaaatgtat
    11161 gatatttcag gtactaatat aactaaaggt gttgaagtat cttcacttga tagaacaact
    11221 aagtgggaat ttaaacctaa aaaatctgtt ggtgataagg ttgtagcagg agatataatt
    11281 ggtggcgttc aagaaacagc agtagtagaa tgtaaaatta tggttcctta tggagttaag
    11341 ggtactataa aagaaatata ttctggtgaa tttactgttg aagatacagt ttgtgttatt
    11401 acagatgaaa aaggtaatga tatacctgta actatgatgc aaaaatggcc agttagaaaa
    11461 gaaagaccat atagagaaaa gaaaacacca gattcacttc ttgttacagg tcaaagagtt
    11521 atagatacat ttttcccaat cacaaaaggt ggggtagcag ctataccggg accttttgga
    11581 agtggaaaaa ctgttacaca gcatcagctt gctaaatggg cagatgcaga tatagttgta
    11641 tatataggct gtggcgagcg tggaaatgaa atgacagatg ttcttcttga atttcctgaa
    11701 ttaaaagacc caagaacagg cgagtcactt atgcaaagaa ctgtgcttat agcaaataca
    11761 tcagatatgc cagttgctgc acgtgaagct tctatataca ctggtattac aatagctgaa
    11821 tattttagag atatgggata tagtgttgca cttatggcag actctacatc aagatgggct
    11881 gaggctctta gagagatgag tggtcgttta gaggagatgc ctggtgaaga aggttatcct
    11941 gcatacttag gttcacgtct tgctcaattc tatgagagag caggaaaggt aaattgtcta
    12001 ggtatggatg aaagagaagg aacacttaca gcaattggtg cagtttctcc tcctggtggt
    12061 gatacatcag aacctgtcag ccaagcaacg cttcgtatag taaaagtatt ctggggtctt
    12121 gatgcatctt tagcatataa acgtcatttc ccagcaatta actggttgac tagttattca
    12181 ctctatcagg aaaaagttga tgtatgggca gataaaaatg taaatgataa tttctcagct
    12241 caaagagctc aagctatgaa acttttacaa gttgaatctg aacttcaaga aatagttcaa
    12301 ttggttgggg ttgattcact ttcagatgga gatagattga tactagaggt tactcgttct
    12361 ataagagaag acttccttca tcaaaactct ttccatgaag ttgatactta tacatcactt
    12421 cataaacaat atagaatgat gggacttata ttaaaattct ataagtcagc acaagatgct
    12481 atcaagcaaa atgttactat aaacgatatt atcaagcttt cagttcttga aaagattggt
    12541 cgtgctaagt atgtagaaga aggcgatata gatgcagctt atgattcaat tgaagatgaa
    12601 atagacaacg agcttgcaga ccttattaaa aagagggggg ctgaagaact atgataaagg
    12661 aatataagtc aatacaagag gtagccggtc ctcttatgtt aataaacggc gtagaaggtg
    12721 taaaatttga tgaacttgga gaaattgaac tttcaaatgg agaaattcgc cgttgcaaag
    12781 ttctagaggt aaatggagat actgcacttg ttcagctttt cgaaagctca acaggtataa
    12841 accttgctga aagtaaagtt cgttttttag gaaagagtct tgactttgga gtttcaccag
    12901 atatattagg ccgtgttttt agtggaatgg gtagacctat tgatggaggt ccagaaatta
    12961 taccagataa aagacttgat ataaatggag ctccaataaa tccagcagca cgtgattatc
    13021 cggccgaatt tatacaaact ggagtctctg ctatagatgg acttaataca ttggttagag
    13081 ggcaaaaact tcctatattc tcagggtctg gtttgccaca tgccaatctt gcagttcaga
    13141 ttgcaagaca ggcaaaagta cgtggaactg attctaagtt cgccgttgta tttgctgcag
    13201 ttggtataac ttttgaagat gctgaatttt ttataagcga ttttaaagca actggtgcaa
    13261 tcgaccgttc tgtagtgttt gtaaaccttg caaatgaccc agctgtagag cgtgtatcta
    13321 cacctagaat ggcacttaca gcagcagaat accttgcttt tgagcaagat atgcatgtac
    13381 ttgttataat aacggatatt acaaactatg cagaggcatt gcgtgaagtt tcagcagcta
    13441 aaaaagaagt tccaggacgt cgtggatatc caggatatct ttatactgac cttgctacta
    13501 tgtatgaaag agcaggaaaa atgaaagggc atgaagggtc tataactatg attccaatac
    13561 ttacaatgcc tgaggatgat aagacacatc caattcctga ccttacagga tacattacag
    13621 aagggcaaat tatacttagc cgtgaacttt ataaaaaaga tatacagcct cctatagatg
    13681 ttttaccatc actttctcgt ctaaaagata aaggtattgg taaaggtaag actcgtgaag
    13741 accatgcaga tacaatgaac cagctttttg cagcttattc aagaggtaag gatgcaaaag
    13801 aacttatggc tatacttggt gaatctgcac tttctgagat tgatttaatg tatgctaagt
    13861 ttgcagatga atttgagaaa gaatatgttt cacaaggatt tagaactgat agaactattg
    13921 agcagacatt agaaatcgga tggaagcttc ttagtatgtt accaaaaagt gaacttaaac
    13981 gtatccgtga tgaatatttt gaaaaatata tgcctaagga gtgatatctg tggctagatt
    14041 aaatataaat ccaacacgta tggaaatgac tagacttaag aaactcctta aaactgctac
    14101 aagaggtcat aagcttctta aagataaact tgatgaactt atgaaacaat ttcttgagat
    14161 tgtaagagaa aataagcgat tgcgtgaaga agcagaaaat gctcttgata ctgcttataa
    14221 aaactttata atcgcaagag cagttatgag tcaggagtat ctgggttcag cacttatgat
    14281 gccaaagcaa tcagtttcag tagatgtttc tacgagaaat ataatgagtg ttgatgttcc
    14341 tgtgtttgac tttaaaactg aaaacaatca aagtgatatt tatccttatg gtcttgcttt
    14401 tacatcaggt gaattagatt ctgctatgga agctttctca gatgctatgc aaccactttt
    14461 aagacttgca gaaagtgaaa aaagtgcaca gcttcttgca caagagatag aaaagacacg
    14521 tagacgtgtt aatgctcttg aaaatgtaat gataccaaat tacattgaga ctattaagta
    14581 tatagctatg aagcttgaag aaaatgagag agcaagtact actagactta tgaaggttaa
    14641 ggatatggtg cttaaaaaag cacttgaaga aaagaaaaaa aatgatttgg ttgtataatt
    14701 aaatacttat aaaaataagt ctaacattct actttataga ggttagactt attttttatg
    14761 tttaaattaa aatgtagata ctataaaaat taatatattg tttaaaaaca gactgtattt
    14821 aactaaaaat aatatattta acaaaaattt ctaaaaatta acgaactgtt aatttacact
    14881 taactttata ttaacaaatt aggtgtatta tataaacata agcaaaaaca ataaaaaaca
    14941 aataaaaaaa ggaaaaaaca caaaggggga agatatgaaa gaatttatat ttacttgtct
    15001 gtgcattgta gtgattacat ttatgacaag ttgcatatca acaacaccac aaactactca
    15061 aggaaaagga aataataaac ttacaatata tgcagcacta gaggaagagc agatagctta
    15121 ttatatggaa ggttttagag aagagtatcc caatatagat gtagagatag ttatggattc
    15181 tcatggtgta atagcttcaa aactattagc agaaaaagat aatccacaag cagatattat
    15241 atggggatta tcagcaatga acatgataga gctaaaaaaa gatggtgcac ttgaagcata
    15301 taagcctaaa aattatgata aagttgataa aaaatttaaa gatactgaag atgatgtatc
    15361 ttgggtagga atgagtgtcc cagagacagc aatagtagtt aattataagg aacttaagaa
    15421 actaggtata gatattccaa aaagttatga agatttaata aaaccagaat ataaaggact
    15481 tataacaatg ccaaatccag catcatctgg aacggggtat cttacagtat cagggcttat
    15541 tcaaatgatg ggtgaaaaag atgcttacaa ttacatggat aaattacata aaaatatagc
    15601 tacatataca cactcaggtt caaaacctgc gaagttagct ggctctggag agtatccaat
    15661 aggtattaca cttggatata gagctacaca acaattatct agtggagaac ctatagaggt
    15721 tgtatttcca aaggaaggtt caggttggga cttagaagca aatgccttag ttaaaaaaga
    15781 taatataaaa aaagaagcaa agctattttt agattgggca ataagtgatt cagctatgga
    15841 tagatatgca catgaagtag cagttacttc cataaagaca tataatccag ttccaaaagg
    15901 ttattctaaa caaccatttg aacagcttat aaaagaagtt gatttaaact ctgctgccaa
    15961 aaacagaagt acagtactta aaacttggga atcaaaatac ggttcaaaat ctgaaagtaa
    16021 gtaaggttta aattaataat ttagatgaaa gaattttaga agaaaaagta ggatataaaa
    16081 tttaaaataa aaaaataact atataaggag agataagata tgagttactt aaaaataaat
    16141 aatgtgttta aaagttatga ccaaaaaagg gttttaaata atattagttt ggatattgaa
    16201 gaaggggaat ttctatgttt actcggacct agtggatgtg gtaaaactac attacttaga
    16261 ataatagcag gtcttgaaga tgtaaatagt ggaactataa ttctccagga taaagatatt
    16321 acaaatttag agccatcaaa gagaggtttt ggtatagtat ttcaatcata tgcattattt
    16381 ccgaatatga ctgcttataa caatatagct tttccactaa aagagagaaa agtttcaaaa
    16441 gaaaaaatag ataataaagt taaggaagta cttgaaacag ttggattgac taatgaagct
    16501 cataaatatc ctaaagcatt atcaggtggg caacaacaga ggattgcaat agcaagagca
    16561 ttagctcttg aaccaaagtt tttactttta gatgaaccaa tgtccgcatt agatgcaaag
    16621 gttagacaca agcttagaat ggacattaaa agattacaaa aagaacttaa tataacgaca
    16681 ataatggtaa cacatgacca agaagaagct ataactatgg cagataaaat agctattcta
    16741 aatggaggag atataatgca aatagggact ccagaagaaa tataccaaaa tcctcaaaac
    16801 ctatttacag ctcaatttat aggagataca aactgttttg ataatggaga ttctatatta
    16861 actgtaagac cagaatatgt tcaaatagag aaatcgacaa aagaaaacta ccaaggtata
    16921 atatcaaata tagagtttag agggaattta ttgagagttg aaataaaaga taaattaaat
    16981 gaaaatttca taataagtga tgtttctata aaagagtggg ttaatttaaa tttagtagaa
    17041 ggtgaccttg taaaaataag tatagacgaa aaatactatt taaagtatcc aaaagttaaa
    17101 ggagcataag tgtatgaata ataatgctat aagttctaaa agtataagtc ttaaatcaaa
    17161 aatgaattta gatataaaaa aatgtatgct tacagtatta gttatgtctt tagtaatatt
    17221 tttattagca cctataatat atctatttgt acaggctttt ttagataaag atggagtctt
    17281 tgtaggatta caaaactttc aagaatactt ggcttctgaa tctctattaa attctctaaa
    17341 aaatacagta tttgtatcag ttatatcaac tgtaatatca ctaacacttg cattagtata
    17401 tgcatatgca ttaactagaa caaagattaa atttaaaggt ttttttaaat cattagctat
    17461 gtttcctatg tttgctccaa caatgatgca tgcaataggt ctagtatatt tatttggaaa
    17521 tcaaggtttg gtaactaatt tacttggagt gcatgttcca atatatggag cagtgggaat
    17581 aatattggca caaattatat atgtatttcc tcaatccttt caaatacttt atatatctct
    17641 aagttcaacc gattatagac tatatgaagc tagtaatact atggggataa gcaagataag
    17701 agaatttata agcataacaa tcccaagtat aaaatactct ttaataagtt ctgcattcgt
    17761 atcatttaca ttatgtttta cagattttgg agctccaaag gtgataggag gagagtttaa
    17821 tgtattatca atagatgtat ataagcaaat tatagggcaa cagaatatgc caatgggagc
    17881 aactgtatca atattacttt taatacctgc aataatatcg tttatagtaa ctaaaaaaat
    17941 tgaaaaaaat caaaactcat atataacttc aaagtcaatt ccatataagg ttaaaactaa
    18001 taagttaaga gatagtattt taggaggggt tgtaggaatt attgcatttg gtataatagc
    18061 tgtaatgtta actgtaatct tatcctcatt aatatcagtt tggccatatg atttaagact
    18121 tacactggaa cacttttcta tgaaaaacag tattgatgga attaaaccat ttataaattc
    18181 aataaaaatg tcactattga cagctattct agggacgagc tttgttttct tatttgcata
    18241 tttaaatgaa aaaacagaaa aagctccata tttaaaatca tttgggtatt ttttaagtac
    18301 attgcctatg gcattgccag gattagtttt aggtatagct tatgtattat tttttaataa
    18361 attagagttt aattttatgg atacaatata cattaaaaat ttgtttaatg gattatatgg
    18421 aacattactt ataatggtta tatgtaatat agttcatttc ttttcagtag catttataac
    18481 agctactact gctattaaga aagtagataa agaattggac atggtagcta aatcaatggg
    18541 aataaacagc gcatcaattc ttaagaacgt aactatacca ctttgtttgc cagcagtttt
    18601 agagaacttt atgtactttt ttctaaactc tatggtgaca atatcagcac tagtttttat
    18661 atatactgca agttcaaaag tagctgcaat atcaattgta caactagatg ataaggggag
    18721 tactgcacag gcagcagcac tagctgtgtt aatattagtc acaaatatat tagtgaaagt
    18781 aatatatgaa tttataaaga atcgtttaga aaaaagaaca aactttaatt aaataattta
    18841 tcttataaaa acacaa
//
LOCUS       DAEPLF010000057        18755 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEPLF010000057 DAEPLF010000000
VERSION     DAEPLF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18755
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.057"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            7..2457
                     /gene="priA"
                     /locus_tag="KR314_001718"
     CDS             7..2457
                     /gene="priA"
                     /locus_tag="KR314_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBH0328105.1"
                     /translation="MYTDNLFTYQIPVFLSDVIKIGHRILVPFGKGNKPTEAFVFQFT
                     DSLDEKIKIKEIIDILDENPIFRKEDLELVYWMKNRYLCTYIECINLIYPKGYKLNNY
                     KVVLLGESLSGLNEIELKEKISTLSDKNREIVEKVIDSKGKIKVDKLKYIPNLNSSLY
                     TMNKNGIIKLCWEYKNHKNEKKVCYISLSLESDKIDDYIEQNKINVGSKQKEILSFLK
                     NNENVEINDLLDLLNASKQSINSLSKKKLITLEFKDYYREPKSIYKSVLKSIKLNNEQ
                     QEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVYMEIIEFALNQGLDSIFLVPEIALTP
                     QTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAVKAGKVRVLIGARSALFAPFNSLGLI
                     IIDECHESSYKSEKNPKFNAIEVARFMALKNNITLILGSATPSIEEYYRAKSGEYKLI
                     NIKSRANDKPLPNIEVVDMKDELDKGNRSIFSLKLQKEIRYAIEENNQVILFLNRRGY
                     ANFVSCRKCGYVFQCENCDISLTYHKKSNTGRCHYCGYEKEIPKECPECKSTYVKPFG
                     VGTQKIEEELKYIFPDIKTLRMDKDTTSKKGALDEILNKFKDKEADVLIGTQMLSKGL
                     DFENVTLVGILSADMILNFPDFKSFETTFQLITQVSGRAGRADKEGKVVLQTYDTEHY
                     AIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNMLSVVVSGEDERLVIKNIKNMHASLIY
                     LLEKRGINDLGFILGPNPCSISKINQNYRWQILFKDENIEINLLKGIIKYICITKRDL
                     IFDKNINVSIDINPNSVL"
     gene            2475..2915
                     /gene="def"
                     /locus_tag="KR314_001719"
     CDS             2475..2915
                     /gene="def"
                     /locus_tag="KR314_001719"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBH0328106.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            2930..3859
                     /locus_tag="KR314_001720"
     CDS             2930..3859
                     /locus_tag="KR314_001720"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBH0328107.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTIYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            3869..4621
                     /locus_tag="KR314_001721"
     CDS             3869..4621
                     /locus_tag="KR314_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBH0328108.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNVGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIDFLTGKNILICN"
     gene            4690..5391
                     /locus_tag="KR314_001722"
     CDS             4690..5391
                     /locus_tag="KR314_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBH0328109.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            5453..6778
                     /gene="rsmB"
                     /locus_tag="KR314_001723"
     CDS             5453..6778
                     /gene="rsmB"
                     /locus_tag="KR314_001723"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBH0328110.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            6786..7817
                     /gene="rlmN"
                     /locus_tag="KR314_001724"
     CDS             6786..7817
                     /gene="rlmN"
                     /locus_tag="KR314_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBH0328111.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGNIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            7837..8589
                     /locus_tag="KR314_001725"
     CDS             7837..8589
                     /locus_tag="KR314_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBH0328112.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            8606..10621
                     /gene="pknB"
                     /locus_tag="KR314_001726"
     CDS             8606..10621
                     /gene="pknB"
                     /locus_tag="KR314_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBH0328113.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     EEEEEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGSSNSGNSGGNSGNNGGNSGGSNSGNSGGSSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            10729..11466
                     /locus_tag="KR314_001727"
     CDS             10729..11466
                     /locus_tag="KR314_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH0328114.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVENMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            11564..12448
                     /gene="rsgA"
                     /locus_tag="KR314_001728"
     CDS             11564..12448
                     /gene="rsgA"
                     /locus_tag="KR314_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBH0328115.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            12453..13124
                     /locus_tag="KR314_001729"
     CDS             12453..13124
                     /locus_tag="KR314_001729"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBH0328116.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            13133..13774
                     /locus_tag="KR314_001730"
     CDS             13133..13774
                     /locus_tag="KR314_001730"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBH0328117.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTVSVTAI
                     KGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            13905..14534
                     /locus_tag="KR314_001731"
     CDS             13905..14534
                     /locus_tag="KR314_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBH0328118.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            14589..15245
                     /locus_tag="KR314_001732"
     CDS             14589..15245
                     /locus_tag="KR314_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0328119.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGASIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            15660..16376
                     /locus_tag="KR314_001733"
     CDS             15660..16376
                     /locus_tag="KR314_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0328120.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMRLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVNIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAINTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            16351..18684
                     /locus_tag="KR314_001734"
     CDS             16351..18684
                     /locus_tag="KR314_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0328121.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLL
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
BASE COUNT         7626 a         1819 c         3531 g         5779 t
ORIGIN      
        1 aatactatat atacagataa tttatttaca tatcaaattc ctgtattttt atctgatgta
       61 ataaaaatcg ggcacagaat attagtacca tttggaaaag gtaataaacc aacagaagcc
      121 tttgtatttc aatttacaga tagcttagat gaaaaaataa aaataaaaga aattattgat
      181 atattagatg aaaatccaat atttagaaaa gaagatttag agttggttta ttggatgaaa
      241 aatagatatt tgtgtactta tatagaatgt ataaatttga tatatccaaa gggatacaaa
      301 ttaaataatt ataaagtagt cctattaggt gaaagtttaa gtggattaaa tgagattgaa
      361 ttgaaggaaa aaatatcaac tttaagtgat aaaaatagag aaatagtaga gaaagtgatt
      421 gatagtaaag gtaaaattaa ggttgataag ttaaaatata taccaaactt aaatagttct
      481 ttgtacacaa tgaataaaaa tggaattata aaattatgtt gggaatataa aaatcacaaa
      541 aatgaaaaga aagtatgtta tatatctttg agtttagaaa gtgataagat tgacgattat
      601 atagagcaaa ataaaataaa tgtgggaagt aaacaaaaag agatattaag ttttcttaaa
      661 aataatgaaa atgtagagat aaatgattta ttggatttat taaatgcttc aaaacaaagt
      721 ataaattctc taagtaagaa aaagctaata accctagaat ttaaggatta ttatagagag
      781 ccaaaaagta tttacaaatc cgtactaaaa agtataaaat taaacaatga acaacaagaa
      841 gcagttgatg agataaagtc gaatatgttt gtagatgata aaaaaccata cttaattcat
      901 ggagtaactg gaagtggaaa aactgaagtt tacatggaaa taatagaatt tgctctaaat
      961 caaggcttag acagtatatt cttagtacct gagatagcat taactcctca aacaatagat
     1021 aggctaaaga gtaggtttgg agatttagtt ggagtgttcc acagtaaact atctgaagga
     1081 gaaaaacatg atgtttacaa agctgtcaaa gcaggtaaag taagagtgtt aattggagct
     1141 aggtcagctt tatttgcacc ttttaattcc ttaggcttaa taataattga tgaatgtcat
     1201 gagtcatcat ataagtctga gaagaatcca aaatttaatg ctatagaagt tgctagattt
     1261 atggcattaa aaaataatat aactcttatt ttaggttcag caacgccctc aattgaagag
     1321 tattataggg caaaatctgg agaatacaaa cttataaata taaaaagtag agcaaatgat
     1381 aagcctcttc caaacattga agtggtagac atgaaagatg aattagataa aggaaataga
     1441 agtatattta gtttgaaatt acaaaaagaa atacgttatg cgattgaaga aaataatcaa
     1501 gtgatactat ttttaaacag aagaggttat gcaaattttg tatcttgtag aaagtgtgga
     1561 tatgtgtttc aatgtgaaaa ttgtgatata tctttaactt atcataagaa aagcaatact
     1621 ggaagatgcc actattgtgg atatgagaaa gaaattccaa aagagtgtcc tgaatgtaaa
     1681 agtacttatg ttaagccatt tggagttgga actcaaaaaa ttgaagagga attaaaatat
     1741 atttttccag atataaaaac tttgagaatg gataaagata caacttcaaa aaaaggtgct
     1801 ttagatgaga tactaaataa atttaaagat aaagaagcag atgtacttat tggaacacag
     1861 atgttaagta aagggctgga ttttgagaat gtaactttag taggtatttt gtctgcagac
     1921 atgatattaa attttccaga ctttaaaagt tttgaaacta catttcaatt gataactcaa
     1981 gtctctggaa gagcagggag agctgataaa gagggaaaag tagtacttca gacttatgac
     2041 actgaacact atgctattaa gcatgctatt gaatatgatt atgagggttt ttatgaagat
     2101 gagataaaaa taagaaaagc atttggatat tctcctttta ataatatgtt aagtgtagtt
     2161 gttagtggtg aagatgaaag attagtgata aaaaatataa aaaatatgca tgcatcattg
     2221 atttatttat tagaaaaaag gggtataaat gatttaggat ttatattagg tccaaatcca
     2281 tgttcaatat caaaaataaa tcaaaattat agatggcaaa tactctttaa agatgaaaat
     2341 attgaaatta atctcttaaa gggtataata aaatatatat gtataacaaa acgagatttg
     2401 atatttgata agaatatcaa tgtatctata gatattaatc caaacagtgt attataaatt
     2461 taggaggaat tataatggct ttaagacaaa tagttcaaat aggagaacca gtattaagaa
     2521 aaaaatcaaa aaaggttgaa aaaattgatg aaaagataat acaattatta gatgatatgg
     2581 cagaaactat gtatgatgca gatggtgttg gactggctgc acctcaagtt ggaatattaa
     2641 aaagagtagt agtaatagat attggagaag agcttataga acttataaat cctgaaataa
     2701 tagaaacatc tggggagcaa atagatgagg aaggatgttt aagtgttgtt ggagaagctg
     2761 gaaatgttag aagaccaaat tatgtaaagg tcagagcttt aaatagaaat ggcgaaacaa
     2821 tagagttaga gggagaagaa cttttagcta gagcattttg tcatgagata gaccacttag
     2881 atggaatttt atttgttgac aaaatagaaa agtagtagga gttgatttga tgaaaatagt
     2941 atttatggga actcctgata tagcagttcc atgtttacaa aaaataatag atgagaagta
     3001 tgaaatattg ggtgttgtaa ctcaaccaga taaaccaaaa ggaagaggta aaaaactagg
     3061 tatgagtcca gtaaaagaac tggcaattga aaataatata cctgtatatc aaccagtaaa
     3121 ggctagagat aaagaattta tagataaaat taaatcttta aatccagatg taatagtagt
     3181 tgtagctttt ggacagatac ttccaaaaga aatattagag attcctaaac ttggatgtat
     3241 aaatgttcat gtttctttac ttccaaaata tagaggtgcg gcacctataa attgggtaat
     3301 aataaatggt gaagaaaaga ctggtgttac aactatgtat atggatgaag ggttagatac
     3361 tggagatatg atactaaaga cagaagtaaa tcttgatgaa aatataacag caggagaact
     3421 tcatgataaa atgatgaata taggagcaga aactttaaaa gaaacattaa gattaattga
     3481 agaaggaaat gctccaagag aagtacaaaa tcatgaagaa ttttcctatg cgccaataat
     3541 gaataaatca ttaggtaaca tagatttttc aaaaagtgcc agagaaatac acaatttagt
     3601 tagaggagta aatccatggc caagtgctta tactatctat aatgatgtca taatgaaagt
     3661 ttggaaaact aaagtgttag atgaaaaaag cacaaaagat gtaggtacaa taattgatgt
     3721 aagcaaagat gggataaaag taagtactat agataatgta cttttaattg aagaaattca
     3781 gatgccgaat aaaaagagaa tgttggttgg tgaatatata aaagggaata ctatagaaac
     3841 tggtttagta ttgggttagg tgaaaagtat gacagatata aagaagtatt taacaagtgt
     3901 tgggattttg attttaatat taggaatttt gataggagtt gttaattttt ttataataag
     3961 tttaactcaa atattctcct tgatgataaa tattataata atatcactaa tacttataat
     4021 actatgttca acagcagtta cttatagagt aattaaagga aagtatgtta actcaaatat
     4081 aatgaaaata aattttaata ttgtcagtgg attatttcct attatatctt tcattgcatc
     4141 atcatttggt atgtcaaaaa gtgatattag aagaatatat ataaagttaa ataatgaata
     4201 tatatacagt aataaatata attttaatcc agaggatata ataattttaa tacctcattg
     4261 tattcaagag aatagttgta aattaaaagt tactaatgat atagataatt gtaaagaatg
     4321 tggaagatgc aacgtaggag aattaattaa gctaaaggaa aaaactaatg taaaaatatt
     4381 tgtagctaca ggaggaaccc tagctagaaa aattataatg gataataaac ctaaagctgt
     4441 tgtagctgtt gcatgtgaaa gagatttgac atctggaata caagatatta agaaaattcc
     4501 tgttttaggt gtatttaaca aaagacctaa tggaccttgt gtaaatacta atgtagacat
     4561 gatggatatt gaaaaagcta tagacttttt aacaggaaaa aatatattaa tttgtaatta
     4621 aactgttttt aagttaagtg aatattaaga ggatgtataa taaaaatata ttttaggaga
     4681 gtgttattta tgtaccctgt atatggcggt ttttggggat ttgacccaac aatggttgta
     4741 ttaattccag ctatattact tactatatat gcacaattta aagtgagttc aacaacaaat
     4801 aagtatttaa gagtaaatac acgtcgagga tatactggag agcaaacagc aagaagagta
     4861 cttgattcaa atggtttgta tgatgtaaag atagaaatgg ttagagggca tctaagtgac
     4921 cattatgacc caagaagaaa ggctgtaagg ttgtcagaag atgtatatta tggaacatct
     4981 ataacttctg ttgcagtagc tgcacatgag tgtggtcatg ccatacaaca tgctaaaggt
     5041 tatgcgcctc ttcaaataag aagtagttta gtaccagtag tgaattttgc ttcaagtata
     5101 tcttggtttt taatattttt aggatttatt atggctggtc catttttaaa aattggtata
     5161 ctattgtttt cggcttcagt gttattccaa ataataacat tgcctgtaga atttaatgct
     5221 tcaagtagag caatcgttca acttgggaat ttaggtatta tagatgaaag tgagtcaaga
     5281 caaagtagaa gagtactttc agcagcagct ttaacatatg ttgcagcagc attggtttca
     5341 atacttcaat tacttagatt gttactaata gctcaaagaa gaaatgacta aaagtaaagt
     5401 gggttgttta gaaacaaccc ttttttatgt ttaataaaag ggagaaattt atatggatgc
     5461 aagagaagtt ggatttaaag tattatgtga tatagaaaaa aatgacaatt attcaaatat
     5521 tgcaataaat aagcatttta aaaatttaga aataagtgat atggatagag gacttgcaac
     5581 agagttaatt tatggagtaa tagaaaataa atattattta gattatataa tcaataaact
     5641 ttcaaaaata aaggtaaaaa aaatgtcaac ttatgttaaa atctttttaa gaatgggaac
     5701 atatcagatt ttatttttag atagtatatc agattatgca gcagttaatg aaactgtaaa
     5761 attatctaag aaatatgata aaaaatcttc tggtttcata aatgctattc ttagaaatga
     5821 aataagagct aaagagacta taatggacat aactgaggaa gatagtgtaa aatacttatc
     5881 tataaagtat tcctataact cttggattat aaaaaactgg atagataaat ttggacaaga
     5941 atttgctgaa gatttgttgg aagcaaataa tgaaaagcct agtatctata taagaacgaa
     6001 tacacttaaa ataagtagag aagaacttat tgaaaaatta agtgaaatgg gtattgtatg
     6061 tttaaaagtt cctatggtag aagaagctat taaagtcgaa aaaatgaaaa atatagaaaa
     6121 aaatgaatta tttaaggctg gtttatttac aattcaagat ataagttcta tgatagtagg
     6181 gaaagtaatt aatccaaaag aaggttcatt tgttttggat gtatgtagtg ctcctggagg
     6241 gaaatctact cacttagcta ctttaatgaa caatacgggt caagtagtag ctagagatat
     6301 ttttgagcat aaactaaagc ttataaactc tactgtaaat agattggggc ttaaaaatgt
     6361 gtttgtagaa agatttgatg cttctgagat tgatgaaaat agtataagta aatttgatta
     6421 tgttttagca gatgttcctt gctctggttt tggtataata agacgtaaac cagaaattaa
     6481 gtataagaaa gaagaagagt taaaagacat cacttctata caaaagaaaa ttttagaaaa
     6541 tgcatctaaa tatgtcaaag ttggtggaac tttagtatat agtacatgta caattcaaga
     6601 tgtggagaat ataaatatag ttacttcttt tgttgaagaa aatcacaact ttgaatttgt
     6661 tccaattgat acagtaaatg tagacttaga caatcaagat aaaggatatt taaagatata
     6721 tcctaatatt catggtatag atggtttttt catagcaaaa ttaaagagaa taaggtagtg
     6781 agagaatgga aaagaaaaag atagtattaa aaaattttac tgaagatgaa cttaaagaat
     6841 ttatgaaaac tatagatgaa aagcccttta gaggaagtca aatattctct tggatatata
     6901 agggtgcaaa aacttttgat gatatgaaca atataccaaa aagtttgaga aataaattag
     6961 aagaggtttc atgtataggg aatatagata tagaattaaa gttagaatct aaggtagata
     7021 atacaaaaaa atatttgttt ttattagatg atggaaatat aatagaaact gtgatgatgg
     7081 attatgatag tagagttact gtctgtgttt ctaaccaagt aggttgtagg atgggatgta
     7141 atttttgtgc atctacaatg gatggattaa ttagaaactt agagccatgg gaaattttag
     7201 accaagttat taaaattcaa gaagatactg gtaagagggt atcaaatctt gtattgatgg
     7261 ggagtggtga gccacttgat aactttgaaa atacaaaaca attcttaaaa ataattaacg
     7321 aaaaaaatgg tcttaatata gggtatagac atataactct ttcaacttgt ggaatagtac
     7381 ctaaaatgta tgagttggca gacttagaaa tagctataaa tttagcttta tcacttcatt
     7441 caccatatga tgaagaacga agaaaaatta tgcctgtagc aaatgcttat tctattgaag
     7501 aaatattgaa tgcatgtaga tattatataa aaaagacaaa tagaagggtt acatttgaat
     7561 attctcttat aaaaggagta aatgactcag aaaaagaagc aaaggcatta gctaagctcc
     7621 taaaaggtat gctatgtcat gtaaatttaa ttccaataaa taaagttgaa gaaagagaat
     7681 acgaaaagcc agataaagca tttatttata aatttaggga tagtttggag aagaacaata
     7741 tacctgccac agttaggatg tctatgggtt ctgatattag tggagcttgt ggtcaattga
     7801 gaagaaaata caagtaattt gtaaggagga agtcgtatgg tttatagttg tgcctcccat
     7861 ataggaaaaa taagaaaaaa caatgaagac tattgtgagg gagaagttat agatacagaa
     7921 catggtccaa taggaatatt tgccatagct gatggaatgg gtggacataa aaaaggtgaa
     7981 gtcgccagta aattagcagt agaaaatata attgattttc taaaggaaaa cttattacaa
     8041 catgataatg taaaaataga ctatatagat gatatattaa aacaggctta taataatgta
     8101 aactcaattg tgcacaaaaa atctatggaa gatatagaat ttgaaggaat gggaactaca
     8161 ttagttacgg ctatagtgta taataatgtc ttatatgtgg caaatgttgg agatagcaga
     8221 tgttatttgt taacagatga aaaatttgat aaaataacta tagaccattc cgtggttgaa
     8281 gaacttatga tggcccatgt cattacagaa gaagaggcaa gaagacatcc acagagaaat
     8341 agaataacta gagcaattgg aacagatgat atggttgttg tagatatttt taagaaagaa
     8401 atcaaaaaaa gtgatataat acttcttgcg acagatggat taactgggtt tattgatgat
     8461 gaagatataa gagatttaat attagattat gaatatgaga gaactagtaa tataagtgaa
     8521 gaattgataa gtatggccaa tgatgtttct ggtaaagata atgtttcggt tatagtgata
     8581 aaagtataat taggacggtg atattgtggg agatacaatt ttaggaaatc gatatgaaat
     8641 catcaggaag attggtgatg gaggaatggc ctttgtatat gaggctaaag acagattatt
     8701 aaacaggact gttgcactta aagtacttag acctgaattt gtagatgatg atgaatttct
     8761 aacaaagttt aaaagagagg cagaagcagt agcaagtctt tcacatccaa atatagtaaa
     8821 tgtttatgat gttggtgaag atggaaaagt tcactacatt gtaatggagt ttgtagatgg
     8881 gaagaatcta aaagagatta tacaagatga aggtatatta gatgaatata ctgcattaga
     8941 tataacaaaa caaatagcta tggcacttag tgcagcgcac aaaaagggga ttatacatag
     9001 agatataaaa cctcataata ttctcatatc aaatgaagga agagtagtaa aagtggctga
     9061 ttttggtata gccaaagctg tttcaaattc aacaatgact aatattggta gtataatagg
     9121 ttcagtgcat tatttttcac cagagcaagc taaaggtaaa tttgtaacta ataatgctga
     9181 cctgtattct ctaggtatag ttttatatga aatgttgata ggaaaagttc catttagggg
     9241 agatagtcca atttctatag cacttcaaca cattaatgat gatatagact ttacatcaga
     9301 agaaaaagtt aggattcctc aaagtgtaag aactacaata aaaaagctta cagaaaaatc
     9361 tagcgcagat agatatcaaa ctgctgaaga attgatagaa gatatagagt atatagaaaa
     9421 aaatattgat ttagatttta taaaagaata tgatgatttt gcaactaaga aaattgatga
     9481 aaaagaaatt aataaagttg taaatccaac acttgcaaaa ccagctcctg aaaaagtggt
     9541 taagcctgtt gaagtagctg atttggatga tgatgaagat tactatgatg atttttatga
     9601 agaagatgaa gaagaagaag aagaagagga agagataatg agagctaaaa agaatcagag
     9661 acctaagagt actccaagta agagaactaa gaaaaagaag aaaaaacagg aaagcccaaa
     9721 atctagaaga agattaaaag taattgcggc tgttttgata ctgatattgt gtgctcaagt
     9781 attcttagca tataagtttt tatttgctgg agggtttggc aataaatctt tgactgttcc
     9841 taatcttgta aatatgacat tagaagaagc tcaaagtgct gttgaaaaag aaggattata
     9901 cctgagtgta aaaagtgaag agtataatag tgaagttgat gaaaattgta taatatcaca
     9961 gacacctgaa ggtggaagta caaatgtcaa aaagggtgat actataaatg ttgtcgtaag
    10021 taaaggttca agtcaggcat ctgttcctaa tgtggtggga cttactttga gtaatgcaaa
    10081 acaacttatt gaagaaaata acttaaaagt gggaactgtt aagtatgaat atagctcaat
    10141 atataaagaa ggtacagttt taagtcagag tccaggtgct ggctcatcaa gagctcagga
    10201 aggtgatgag gtaaacctat atgtaagcaa aggttcagaa aaatcaaata cacagactcc
    10261 aacagtacca gataaaaaac caacaacacc agagaataat acaccaacag aacctggttc
    10321 aaactcaggg aacagtggtg gaaattcagg aagttcaaac tcaggaaata gtggaggaaa
    10381 ctcaggcaat aatggaggta actcaggagg ttcaaactca ggaaatagtg gaggaagctc
    10441 aggaggctca aactcaggag ataatggagg aagttcagga ggttcaaact caggagataa
    10501 tggaggaagt tcaggaggtt caaactcagg agacaatgga gatagttcag gaggctcaaa
    10561 ctcaggagat aatggaggaa cttcatcagg aactggcgca aacattggta aaacagaata
    10621 aatattaata aaataagagc tgtcctttta tagaaaatat tttctttaaa gggcagtttg
    10681 ttttgtataa aatactgaat tggttataat ataaggagaa aagtatttat gaagaagaaa
    10741 accgttttaa ttacaggagg agctagaggg attggtaaag ctatgtctaa agcttttgca
    10801 aaagaaggtt acaatgtatt agtaaatttt aataagtcag aaaatgaagc taaagagttg
    10861 tatactatat taaatgaaaa aaatttttct gtaaaacttt ttaaggctga tatttcaaat
    10921 agagaagatg tagagaatat ggtagattat tgtataaaag aatttggagg attggatgta
    10981 cttgtaaaca atgctggtgt aagtcaagat aagttgttta cagatataac cgatgaagat
    11041 tgggataata tgatgaatat taatttaaaa ggaagctttt attgttcgca agttgcttta
    11101 aaatacatga tatcagaaaa aaaaggaaat ataattaata tatcttcaat ctggggtata
    11161 tcaggtgcgt cttgtgaagt gcattattct ataacaaaag caggtataat aggtatgaca
    11221 aaggcattag ctaaagaagt aggaccatca aatattagag taaatagcat agctccaggt
    11281 gtcattaata ctgacatgtt atctggatat aatgaggaag atatagacgc tcttgtggaa
    11341 gaaacccctc taatgaggct aggaactcca gaagatatag caaattgtgc tattttcttg
    11401 gcatcagaca aatcaaattt tatcactggt caggtaatta gtccaaacgg aggatttgtt
    11461 atttaaaaaa aatgtggtat tatataaaaa taggaaagaa taacacgata tactattcat
    11521 aaacagtatg tgtttatata aattttataa agggaggcat ttgatgctag aaggaaaaat
    11581 tataaagggg attagtggat tttattatgt agatacttat aatggtattt atgaatgtaa
    11641 ggctagaggg atatttagaa agcagaaaat aacacctttg gttggtgata gagtcaaaat
    11701 aagcattgtt gatgaagatg aaaaaaaggg tatcttagaa gaaatcgaca gtagagatac
    11761 agaacttata agacctccta ttgcaaatgt agataaagca ttaatagtat tcgcaataaa
    11821 aaatcctaaa ccaaacttat ctcttttaga tagatttata gttttggcag aaaaagaaaa
    11881 tttagaaact gtaataatcc ttacaaaagc agatttagat gataatgata cattagaaac
    11941 tgttaaaaat atatatgaac ttagtggcta taaagtcatt cctgtaagca atattactaa
    12001 gttaaatata gataaagtaa aagaagaatt aaaagaaaat gtagtagtgt ttgcaggacc
    12061 atctggagtt ggaaaatcaa gcttattaaa tgaaatagat gaaaatttta aacttcaaac
    12121 aggagtggta agtgataaaa taaaaagggg aaaacatact actcgtcacg ctgaactttt
    12181 aaaattggaa tttggtggaa tggttgcaga tacaccaggt tttagttccc ttgcacttga
    12241 agatatagag gaagttgaac ttaaagatta ttttatagaa tttgataaat ttaatgattg
    12301 taagtttggt tcaaagtgta ttcatgaaaa tgaacctaat tgtgctataa aagaagctgt
    12361 tacaaatgga gaaatatcta aagaaagata tgatagttat atacagctgc tacatgaaat
    12421 aagacaaaat aatagtagga ggtattagaa aaatgattaa attagcacca tcaatattat
    12481 cagcagattt tgcaaaatta ttagaagatg taagaaaagt agaaagtgca ggatgtgaat
    12541 atcttcatat cgatgtaatg gatggacact ttgtaccaaa cattacatta ggaccacttg
    12601 tagtcaaaag tttaaagaaa gaaaatataa atatggtatt tgatgctcat cttatgatag
    12661 aaaatccaga ccagtatatt gaagaatttg taaaagcagg atgtgatata ataactgttc
    12721 accaggaggc ttgtgttcat ctacatagaa ctatacaaaa tataaaatca catggaataa
    12781 aagctggtgt agtacttaat ccagctacac cagtagatac gattaaacat gtactacctg
    12841 atttagatat ggtattatta atgtctgtta accctggatt tggaggacaa tcatttattc
    12901 cttgtgtttt agataagatt aaagagttaa aagctattat agatagtcaa ggattaaaca
    12961 ttgatataga agttgatggt ggtataagcc ctaaaaacgt agccgaagtt gtacaagcag
    13021 gagctaatgt aatagtagca ggttctgcaa tatttggaag tgatgacatt caagaaacag
    13081 ttaatttatt tagaaagaat gcttctcttg aagaattagt ttaaatttaa taatgaaaat
    13141 ctgtattgtt ttaaatggcg aaattgaaga ttacgaagtt acaagagata ttataattaa
    13201 ggaatgttat gactgcataa tatgtgcaga tggaggggca aatcacactt ataaaatgga
    13261 aataatgcca gattatatat taggagattt agattcagta gaagaagaaa agataaactt
    13321 ctataaaaat aaaggtgtta aatttgaaaa atttccatct aaaaaagatg agactgatac
    13381 agagctttgt ctttttttag ctaagacact taaagcaaat catattgatt tttttggtgc
    13441 tttaggggga aggattgacc acactcttgc aaatataaag ctattatatt atttaaaaga
    13501 agatggtata tattctagga tactttctga taaagaagag atgtatatag ttgaaaatga
    13561 ggaaatttca ttgtatggaa acccagggga cactgtttct gtaacagcta taaaaggtga
    13621 tgctaagggt gtaacactta ctggcttaga ataccctctc gataattatt atatgaaata
    13681 ctctgtacct attggaatat caaatgttat gttgagtaat tcttgtaaga ttaaagtaga
    13741 gcaaggctgt gtacttgtag ttagaaattt ataaaatttg attttgacaa ttggttttat
    13801 attgtagagc agatacaatc aagtaaaata tatttatgat tgtatctgct ttttagtata
    13861 taattataaa tatataataa actttgacat aagggggatt ttttgtggta gatagtatta
    13921 tttttgattt agatggaaca ttgtgggatt caactgaaga agtatgcaaa gtctggcagg
    13981 atgttttaag taaacataag gaaatagaat taagtgtcac aaaagattta tttagaagtc
    14041 ttatggggct ttcttttgag gaaatagcaa atcgtttatt tccaaattta agtgaagaag
    14101 aaagaatgaa tttattgaat gaatgttctt tgaaagaatg tgactattta tctgaaaatg
    14161 gagctaaatt atttgatgat atagaagata ctcttaaatt actatctaaa aaatataaat
    14221 tatttatagt aagtaattgt caagctggat atatagaatc ttttttaaaa gcacataaac
    14281 tagaacagta ttttatagat tttgaatgtc ctggtaatac tggtttgcat aaaggagaaa
    14341 ataataaact tataattgaa agaaataaat taataaatcc aatctatgtt ggtgatacgc
    14401 aaggtgatgc aaatagtgct aagtttgtag gtataccttt tatatatgcc aaatatgggt
    14461 ttgggaatgt agatgtatat gattatttta tagattcatt taaagatttg ttagaacatg
    14521 atatattaaa ataagatgag tttaaaaatt atattaaaat aataaaaaag ttatgggggg
    14581 atttaaaaat ggaattacaa gatactattt ttaaaagaca aagtgtaaga aagtttaaaa
    14641 atcaagatgt atcagatgaa gatattttaa aaatgataaa agcagcaggt gcagcaccat
    14701 caggcaagaa tattcaaaac tggcattttg ttgttataaa acgccgtgat ttaatggaaa
    14761 agatagcaga tgtgataact aaaaagcaac aagaaatact tgtggaaatg gataaagtat
    14821 cagtggataa ggctaataga tttagaaaat ttgttaaaaa ctttacacta ttttacttaa
    14881 aagctccagt tttagtatta gtttttacaa aggtatataa tccatcagga tattatgaat
    14941 tagagcttat agatgcacct aaggagacta tagataaatt atttataaga aatccaggta
    15001 tgcagagtct aggtgcttca attgaaaact ttacactatc agcaattgaa cttggatatg
    15061 gttcttgttg gttgacaagt caaaactatg cagcagatga gatagaagct gttttagaag
    15121 cagaaactgg ttttgaaaag ggagagtatt ttttaggtgc aatgttagct cttggagtgc
    15181 cagaggacaa tttgaagagt ccatctaaaa aaccagtaga agaaatatgt acatttataa
    15241 aataaaagtc aaggacattt taaaatcaaa gtaaattaaa aacaagataa attaaaaata
    15301 aatatttaaa aaattaaaaa gtaggtttta ttgatattaa ttttatattt caaatcattg
    15361 atttaattaa tacaataaga actacttttt tttatatatt aattctaaat atatttaaat
    15421 ctttagaaaa tctttagaaa agtatatgta atatcttaag tttttaattt taatattaag
    15481 tatataaaac aaaatgaaaa aatattaagt ttaaactaga aggaggtttc taagatggac
    15541 attaaataca aataaccaga acaaaagata taaatatata tataaatgga aaaacttcta
    15601 tcatattgta taatcatata tgtaaagaaa atttataaat taatataaag gagattaaaa
    15661 tgaattcata taatattttg gtggtagaag atgaaaagga gatcgcagat gctatagaga
    15721 tatatctatt aaatcaagga tataatgttt ttaagggata caatggatta gaaggtctta
    15781 aggttataga aaaccaagaa atacatttag ctatagttga tataatgatg cctaaaatgg
    15841 atggtataac acttacaatg aggcttagag aaaactataa ttttccagta ataatgctat
    15901 ctgcaaaatc agaagaagtt gacaaaataa tggggcttaa tataggagca gacgattatg
    15961 taacaaaacc ttttaagcca ttagaactat tggctagagt taattcacaa cttagaaggt
    16021 acactaagta tctaaacatg gttggaaata aagagcaaaa ggtagaaagt gatgatagtg
    16081 tgtttgcaat aggcggatta gaattaaatg aaaatacaaa agaagttaat attgatggaa
    16141 aatatataaa aacaactcct atagaattta aaatactaag tttacttatg agaaatgcag
    16201 gaagagtttt ttctgctgat gaaatatatg aaagagtttg gaatgaaaat gctataaata
    16261 cagatacagt tatggtacat gtgaggaata taagggaaaa gatagaaatt gacccaaaaa
    16321 atcctaaata tttaaaggtg gtgtggggtg ttggatacaa aattgaaaaa atttaaagat
    16381 aaatatatag ttactattat agtttttata cttatgttat cagcatcatt aggtatgatt
    16441 agtcaatatt ctactataca agcaggtgct aaaggtggag caaagaatcc atttgaacag
    16501 gaaagctttg tagatagtat atataaagga agttatgttt tatatcataa tatgaaggaa
    16561 gaacaagaag gtaagatgat acaaccatca aagctattta tcaatgaaga aactatgaac
    16621 tatataaaaa atgtaagtaa agaaaaagat acttatgatg gttcagaaac ttattataca
    16681 gatgatagta ttaatgcaga ttttaatgaa aactttgaaa attgggagca tttcattaaa
    16741 gcgtcaggaa agaatttaaa gtactattta gttgataaag aaagcaattt tgaatttttt
    16801 aatgataata aagaattagg agtacttgat acaacaaaga atgatgcttc aaaagaaaat
    16861 tctgatgaaa atcagtcaaa aaatacagaa atgaatgatg aggaaagaca aaagcagatt
    16921 acagatataa aggaaaacta tagattctac gttgtaatga attttgataa agatggaaaa
    16981 gtaaatattg tggattctta tggagtaaat caaaaagtta ttaatcaaat gttgttatct
    17041 aaagcaagag aagatttaat ggatacaagt ttaggaggaa attcaatttc atataagtta
    17101 tctccaataa aaaatataac attagtatat gctgtaccta aagtaataag taaaggtgat
    17161 agtgtagagt atggttatgg aaatacttat tttgatgatg gtataatttc atatataagc
    17221 aatgtagaaa ataattctta ttataagatt tctggaatga ttttaaaaat tatattagct
    17281 atagttgtta ttgtagcttt agcattccca tatagaaaat ctaaagagtt gctaggattt
    17341 gatacattat taagaatacc gttagagata ctctttattg ctcttgctat tatacagggg
    17401 cttgtagatt tatatcctac tgttataata agtaatacct tgagtgggaa ttatgtagag
    17461 taccttataa acaatgacat aagtgctaaa atggctagca tattggtaag tttaatgaat
    17521 ttaacttatt ggtttgtaat attctcaata atatttgtat ttataatact tttaaaacat
    17581 attttaaatg ttggaataag agagtatttt acaaaacata ctgtgacagg aatgtttttt
    17641 gtatggttaa aaaaagcagg aaaaacagtt attaatcaaa ttagaacaat aaatctaaaa
    17701 gaaaagccaa ataaaactat agttacaatt ttgggaataa atttattgat tatagttata
    17761 atgtgtagta tgtggttctt tggaataata ctcgcattaa tatattcagt agtattattt
    17821 aagatactgt cagattactc taaaaagaca atatatgaat ataatcaact tttagatgtt
    17881 acaaagaaaa tttcagatgg aaacttagat gtaaatatgg aaaaagattt aggatttttt
    17941 aatcctataa aagacgaatt aggaaatatt caaactggat ttaaaaaagc tgttgacgaa
    18001 gaggttaaga gtcaaaagat gaaaacagag cttatatcaa atgtatcaca tgacttaaaa
    18061 acacctctaa catctataat cacttatata gatttattaa aagatgaaag tataacagat
    18121 gaaaatcgta agttgtatat tgatacatta gacagaaagt cacaaagatt gcaacatcta
    18181 atagaagatt tgtttgaagt aagcaaggca aatagtggag atgtttattt gaatatagta
    18241 aatgtggata ttgtatctct aatgaagcag acacttttag aattagatga caaacttgca
    18301 gattcatctc taatagtaaa gaataacttt tctaatgaaa aaataatatt accattagat
    18361 agtcagcgta catttagagt atttgaaaat cttataataa atataagcaa atatgcaatg
    18421 ccaaattcaa gagtgtatat tgatatttta gaaacagata ataaagtaaa tattatgctt
    18481 aaaaatatgt ctgcatctga gattgatttt agtgtagatg atataatgga gagatttgta
    18541 agaggagata aatcacgtaa cacagaaggt tctggtcttg gtttagcaat agcaaaaagt
    18601 tttatagaat tacagggtgg taagatgaat ataagtgtgg atggtgattt atttaaagtt
    18661 acaattactt ttaacaaaaa ttagattaga cttctttaat aattataaag tctatagaat
    18721 aaaagagtct ggaaatattt atgtgtattc tttaa
//
LOCUS       DAEPLF010000058        18283 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEPLF010000058 DAEPLF010000000
VERSION     DAEPLF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.058"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(247..1344)
                     /locus_tag="KR314_001735"
     CDS             complement(247..1344)
                     /locus_tag="KR314_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBH0328122.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILFTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            1637..3163
                     /gene="glpK"
                     /locus_tag="KR314_001736"
     CDS             1637..3163
                     /gene="glpK"
                     /locus_tag="KR314_001736"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBH0328123.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFESGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKEIKMLRVDGGITKNNFTMQFQSDILDIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTKQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            3204..4319
                     /locus_tag="KR314_001737"
     CDS             3204..4319
                     /locus_tag="KR314_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBH0328124.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(4600..5364)
                     /locus_tag="KR314_001738"
     CDS             complement(4600..5364)
                     /locus_tag="KR314_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH0328125.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            5623..5713
                     /locus_tag="KR314_001739"
     tRNA            5623..5713
                     /locus_tag="KR314_001739"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5657..5659,aa:Ser,seq:gct)
     gene            5801..6589
                     /locus_tag="KR314_001740"
     CDS             5801..6589
                     /locus_tag="KR314_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBH0328126.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHIGVLFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSVDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            6610..7350
                     /locus_tag="KR314_001741"
     CDS             6610..7350
                     /locus_tag="KR314_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBH0328127.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDSLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAVVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            7528..7902
                     /locus_tag="KR314_001742"
     CDS             7528..7902
                     /locus_tag="KR314_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328128.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKDNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            7994..9454
                     /locus_tag="KR314_001743"
     CDS             7994..9454
                     /locus_tag="KR314_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBH0328129.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTIGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIEEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLNVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFETWFESFKAELENAIKK"
     gene            9507..12383
                     /locus_tag="KR314_001744"
     CDS             9507..12383
                     /locus_tag="KR314_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0328130.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKILNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILNDKNINDKPIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQEGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRIEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKDKKLKWIRMNIFEDELKVLG
                     VVVDVTKEFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLD
                     NLKYINDTYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRD
                     IINSVKKQMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTI
                     KGEVSEFDINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPK
                     IKTFSSPIDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLS
                     KVDFEEIEDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNG
                     DAVLLYIMPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVISEGVETKGEME
                     TLINLGVDYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            12460..14526
                     /locus_tag="KR314_001745"
     CDS             12460..14526
                     /locus_tag="KR314_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBH0328131.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVYTANRLIDKNKNRYEKVIKCSQGDGGSVTYISPHDSEEEAL
                     YIAKEIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    15118..15328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15554..16369
                     /locus_tag="KR314_001746"
     CDS             15554..16369
                     /locus_tag="KR314_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH0328132.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            16397..17074
                     /locus_tag="KR314_001747"
     CDS             16397..17074
                     /locus_tag="KR314_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH0328133.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            17133..17855
                     /locus_tag="KR314_001748"
     CDS             17133..17855
                     /locus_tag="KR314_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBH0328134.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         7199 a         1929 c         3244 g         5911 t
ORIGIN      
        1 tgaataaaaa acgattgcgt caaaaataaa aaacaattaa atgtaagaat aattatgtca
       61 aaaatataaa ataaattaaa agaaaaagat tatactgaag gaaaaagatt tgaaataggg
      121 gtgaacttta catattatag ttcaccctta tacaaaaata taaaatataa tttattatag
      181 gaaaatttaa aaaaatcagt ctatttaatt tatactgatg tattgtagga ttttacaaac
      241 aaactgttat ataacttttt ttatctccaa ttttgatttt aagatatcta agaatttata
      301 acaatcttct ggatatattt ctcccatatt tgcaacttgg aatatgttag catctaggaa
      361 tttgcctttt cctggataga caacatagcc actttccctt tctaggccta atataaactc
      421 atttacattg atttctttag gtagaaatac agatgttact gtattagaca tatacttatc
      481 tggaagtaaa aatgttagcc ccaattcttc catgccattc cttaaaatag cagaacattc
      541 tttgtatctt tctattttac tgtcatcttc caaaaactca gttaaagctt cattaagggc
      601 aaatattaaa gtaacattag gtgtatttgg tgtttgagaa gatgatttag caactttata
      661 gtgtttataa agattaagat atacattctt acattgattt tctttaatat tttgtaaaat
      721 actctcttta gcacaaaggt atgcagaacc tggaaatgct ccaagagctt taccaccaac
      781 acctgttatt atatctatat taaactcttc tacatcaata tattcacctg ctgaggcact
      841 tattgcatct acaaaaaata ttttgttata cttcttagca agttttccta gttcagggac
      901 tggatttatc attcctgaac tagtttcatg gaacaccata gcaattactt ttatatcaga
      961 attttcaatt atatattttt ctaaaatgtc caggtctggg tattctgccc attcaaaatt
     1021 aggcttatat gtttttacat tataagtaaa taatatttct tctagccttt ctccaaaaac
     1081 accattacta actagcagaa ctgcatcttc tttgtcaaag atagaagata gtacacattc
     1141 atttacagat gttccagagc cagatacaat taatgagtag taattagaag ttgcattata
     1201 tagtttattg attttttgtt gtgtatcttt gaataaatcc ataaactcgt ttcctctatg
     1261 gcatatatca tagtgaagaa gtgaatttct gactttatct gtaaccatta ctggtccagg
     1321 actgaataga tatgtttttt tcattgtaaa gcctccttaa aaagttaaat ttaaattttc
     1381 gaatgactga ctatgtgtaa atattaaaat attgtgtgtt attttaacta gattatagaa
     1441 taaaataatg aatattgcaa taatatttca ataaaaatac ttttttaatc accataatta
     1501 aaaataacaa aaaatattgt aatgaatatt gcataaatcc acatgatgat ataaaatata
     1561 aataacttga atagattata tattcaaaaa ataaaaaaga atttattaat tctgaaaata
     1621 aagggggaca ttttaagtga aatatgtatt agcaattgac caaggaacaa caggaacaag
     1681 agcagtattg ataaacgaaa aatcagagtt tgtttttagc gattatatgg aacatactca
     1741 aatatatcca caatctggat atgttgaaca tgaccctata gaaatttgga ataatacaaa
     1801 aactgtgagt aataatgtat tacaacaggc ttttgaaagt ggaatcaatg aaagtgatat
     1861 aaaaggaatt ggtatagata atcaaggtga aacagtaatg ttatgggata aaaatacagg
     1921 taaaccactt tacaatgcta tcgtatggca gtgtaggaga acttatgatt atgtggaaaa
     1981 tttaaagaat ataccaggtt tagaaaataa gataatagat aagactgggt taattataga
     2041 cccatatttc tcaggtacaa aaattaaatg ggtaatagat aatgtaaaag gtgtaaaaga
     2101 aaaaattaaa aatggagaag tacttgcagg aacattggat agttggttaa tatggaaaat
     2161 gacaggagga aaatcttttt taactgatgt atctacagca gcaagaacaa tgctttttaa
     2221 tataagagat atggattggg atagagagtt gcttgacatt ataggagtgc ctagaagtat
     2281 tttagcagat ataatgccta ctagtggtga ttttggaact acagataagg aggcattttg
     2341 tggggtttct ataaaaatta ctgcttcaat ggtagaccaa ccagcagctt tatttggcaa
     2401 tggatgtttt gaaccaggaa tggttaaaaa tacttatggt acaggatgtt tcttatatat
     2461 gaatataggt gaggagttaa atatagcagg taatggtatt ttgacaacag ttgcttggaa
     2521 gataggagat aaagttacat atgcttatga tggtggagtg tatatagcag gtgctgcaat
     2581 acaatggctt agagatggaa ttggagttat aaaaaattat tcagaaactg atgacatggc
     2641 aaattccata tcaagtactg gaggggtata ttttgtacca gcatttgcag gtatagcagc
     2701 accttattgg gaccaatatg ctagaggcac aatggttggg ataacaggaa gtactaaaaa
     2761 agaacatata gtaagggcta ctcttgagtc tgttgcactt caagtcaagg atgttgttga
     2821 ttccatgaat ttggcttcag ggaaagaaat aaaaatgttg agagtagatg gtggaattac
     2881 taaaaataac tttacaatgc aatttcaatc tgacatatta gatataccaa ttgaaattgc
     2941 agaaaatcct gaaacgacag tacttggtgt agggtatatg gcagggcttt cctgtggtgt
     3001 ttggaacagt attgatgaaa ttagaagtga atttacaata agcaaaaaat atattccaaa
     3061 tatgactaag caaaaaagag aagaaatatt acttggatgg aatcaagcag taaaaagagc
     3121 tatgaattgg gaaaacgata ccaaaaaggt gtgtgctaat taagttacaa tagagtattt
     3181 tacatgtata ggaggaatta aaaatgccct ttgataaaat tatattatac ataatggctg
     3241 tgggagtgct tttaggagcg ttagatagaa taattggaaa taaatttgga cttggagagc
     3301 agtttgaaga agggtttaat tcaatgggtc cattggcttt aggaatggtt ggtatagttt
     3361 gtttagctcc agtaatttca gatgtactag gtcctataat aataccaata ttcaatctct
     3421 gtggagctga cccatctatg tttgcaacta ttttagcgaa tgatatggga gggtatccat
     3481 tggctatgga acttgcacac aataaagagg caggaatgct tgcaggatta gtcgtgtcat
     3541 ccatgcttgg atgtacatta gtattttcaa ttccagtagg actaggtctt atagaatatg
     3601 aagacagacc attctttgca aaagggttac tgataggctt gataacaata ccttttggag
     3661 gtattatagg aggattaata gcaggattta acttatcaat ggtaattata aatatgatac
     3721 cagtgataat attggcagta atattggcag taggtttaaa atttaattct acacttatga
     3781 taaatgggtc attagttttt ggaaaattta tatctatatt gataacgata ggtttgggag
     3841 cagcagcatt tcaagaaata acaggtatag tacttataaa aggcatgaca cctataagag
     3901 aaggtattca agtaattgga agtattgcaa tagtgttatt aggtactttt ccaatattac
     3961 atttattagt taaagttctt aataaaccac ttacaatagt gggtggaaaa ttaggtatgg
     4021 atgccactag tgcagcagga ttagttttta ctttagccaa tagtattcca gtatataaaa
     4081 tgatgaaaga tatgagtcca aaggggaaag tagtaaatac tgcttggctg gtttgtgcta
     4141 cagcagcttt gggggaccat ttaggattta cagcaggagt agttccagag atgattacac
     4201 ctgttgtaat tggaaaattg gttggaggag tattggcaat tgcacttgct atacttatga
     4261 caaaagattt aactttggaa gaagaacaaa gtattaaaat gaagaaagat gcagtgtaga
     4321 ataaacaata cttaagtcaa atttaagtta ttttaaagta ggagtttaaa taactaaaaa
     4381 taaattaaaa agacagacaa ttaaaaaata atcgaataac caaaactgat aaattaaata
     4441 aaatggcaat caaataacta aaaatgataa gctaactgaa agtagttaca taattaaaat
     4501 ataaagtata gtttagacat aagtggagct aaataaataa ttatttggct ccttaatttt
     4561 aatttattac atcaaggaac taaaaatcaa tcatacaagc taattatcag ctatgactaa
     4621 aggtattcct ttttgagaaa actctattat tatattatgg tctatactag cagaggtaat
     4681 aatagtatct atgtgttcta taggagctac tgatgcaaat gaatttatac caaacttagg
     4741 aggttcgaca actactatag attttctaga cctttcaatc attttttttc taaccagtgc
     4801 ttcttctaag tgatagtcag agattccatt ttcaagagtg atacctccag cacctaaaat
     4861 agctttatca acattaaatt gttcaatagc tgagagtgta agtggaccag aaaaagaacc
     4921 ttcagtttct ctaagatagc caccaagcac atatactttt atattagtat tcataagttc
     4981 atttgcaact aaaattgagt tggttattac agttagattc tttttatctt tcaaattttt
     5041 tgaaacttca agtacagtag ttccagtatc cataaaaata gtatcatttg tatttataaa
     5101 ctcactacat ttaatagcaa tctgtttttt atcgtttgag tttatttttt ttcttttaac
     5161 atagttaagt tctacgctac ttgctataac tggtatagca ccaccataaa ctctttttaa
     5221 aagaccgata ctttccatat gggctaaatc tcttcgtgca gtttctattg atatatcaaa
     5281 catttgaact atgtcattta ttttaattat attatctgat ttgagaatac ttaaaatctt
     5341 ctcgtatctt tcctgtggta acataaaata tcctccttgg actagtatta ttttagtttt
     5401 ggcataactt acaattaagt cagagtttag gtatatttgt taatgtatgt atatttatca
     5461 aataatatta taagtgttat gcaagataat tttaccatat ttaaaactta catttaagca
     5521 aaaaagtaaa aatagatgtt taataattca aaataactca aattttataa aacaattgac
     5581 taaaatatga ttttattata taatttacta gtatagagat atggagaagt actcaagtgg
     5641 ctcaagagga tcccctgcta agggattagg ctgggtaacc ggtgcgaggg ttcgaatccc
     5701 tccttctccg ccaattgtta aaggctattt ttattaaaga tagtcttttt ataatgtcat
     5761 aaatgattag atatgtataa ttaaagaaag gagggaatag atgacaactt taattttttt
     5821 catagtgggg atagcatctt tggtagttgc ttttgttatt gatgggggac atattggagt
     5881 attatttgta ttttcatcag cacttgttgt gtttggtgga actataggcg ctgttggagc
     5941 ttcgactcca ttccctgtgt ttaaaaagtc tataaagata atgttattag cttttaaaaa
     6001 taaaaagcct aataacatag aaaatataat ctatttcaaa gaaattgcaa caaaggctag
     6061 gaaagagggg cttcttagtg ttgacaaaga aataagtgag aatgatgaat tagacccttt
     6121 tattagaaaa gggcttgaac ttatggtaga tggggtggat gcagttacta taagaaatat
     6181 attggaatca cagatacaga taacatcaaa acgacataaa gaaggaatag caatttttga
     6241 ttcagcaggt ggatatgcac ctacactggg gataataggt acagttatgg gattagtaca
     6301 tattttaggt aatctttcaa gtaatccaga tgcattagga cctcaaattt ctgttgcatt
     6361 tttggctact ttgtatggtg ttggttctgc caatttaata tggttaccaa tagcatcaaa
     6421 acttaaggct ataaatcaac aggaaataag tgaaagactt ttggtgatag aagcaatatt
     6481 atccatactt gagggtgata attctaattt aatgacagaa aaattaaaaa tatttttaaa
     6541 ttcaaaagaa ctaattgaac ttgaagaaat tggtggtaga gctgaataat ataaaaggat
     6601 ggaatgttta tgttgtatga tgaagatgaa aaagaagaaa acaatgagcg atggctttta
     6661 acttattcag acctcataac attacttatg attttctttg taataatgta ttctatgagt
     6721 aatgttgatg cggagaaata taaacaatta tctcaatctc taaattctgc ttttggtggt
     6781 tcttctggag ttattgaagg tggtaatagc aaaatagaac cagtagttga accaggaagt
     6841 aatgatttag atagtttaca aaatgctaag tttaaaaaag ttggtgaaga aatccagaag
     6901 tatttaaatg aaaatggtat ggctaattca gtatcattaa gagttcaaga tagaggatta
     6961 gtaataagcc ttaaagatac aatactattt gatacaggaa aagcagttgt aaaggataat
     7021 tctagagata agattattca aataggtaag atgttaaatg aaatgaatag ttacatgaga
     7081 gtagaaggtc atacagacaa tatgtctata aaaaattcag agtttaagtc aaattgggat
     7141 ttatcagtta tgagagcaac caatgttgtt caacttctta ttgacaatgc aggtatagca
     7201 ccagataaat tatctgctgt aggatatggt gagtttagac caatagcaga aaattcatct
     7261 gaagaaggta ggtcaaaaaa tagaagagtt gatatagttt tagttgatag taaatatgat
     7321 aatgtagaaa atgtttcaaa ggataaatag ttaaattagc tgtctcaata cagaattaat
     7381 tctaaaggag atggcttttt tatgcgaaaa ttaaaaatag atacatagta agttaccttt
     7441 ttgtaggaat atatggtatc ttaaatatat aatatttaaa taatatataa gctcaattaa
     7501 aaaaatttta tagaggatgg gaaataaatg gggaataaaa ataaaaatac gaaatataaa
     7561 gataaaacaa gagaaagata taataaaaaa gttgagaatt taaataaaga aaaattaaat
     7621 aaagataata aacagtctaa gaaggtagat aagattgatg atattattaa taaatctagt
     7681 attgtattat gtgtacttct agtggtgttt tcattacttt acttatttaa attggttcaa
     7741 cttaatataa taatgatact tttagtaatt acagtagcta ttcaacaagg attggtgtca
     7801 tttggtgcat ttagaaataa aaattatgta aaagctttat tttctatatt tgtaatagta
     7861 tgtttcttaa ttgcttttaa atatgttgta aatcctgttt aaatatatca ataaacgttc
     7921 tataattaat ataaatagtg aagttacagt tttaatgcta ttataaattt tataatttga
     7981 aggagtagat tttatgaata ggtgtaagtt tgcaaaatta attatcacat caatgattgt
     8041 tttattactt acaggatgta gtttaaaaaa ggaagataat tataagttag acccagaaaa
     8101 tccgacatca atacaaattt ggcattatta taatggacag caaaaaatgg cttttgataa
     8161 gcttgttaag gaatttaatg acactatagg tgctgaaaaa ggaattattg ttgaagcgtt
     8221 tagtcaaggt aatgtagttg atttatcaga gaaagtttta gattctgcaa ataaaaaagt
     8281 tggctctgaa gatgtgccaa atatatttgc agcatactca gatactgcat atgaaatgga
     8341 taaattaggg ttactagtag atatggataa gtacataagt aaagaagaat taaatgaata
     8401 catagacgaa tatgtagaag aaggcagaat aaattctaag gatgagttta aacttttccc
     8461 aattgcaaaa tcaactgaaa ttttgatgtt aaataaaact gattgggata aatttgcaaa
     8521 agctacaaat tcaaatataa atgatataaa aacaattgaa ggattagtag agacatcaaa
     8581 aaaatattat gaatggacag atagtttaac gaaagagcct aatgatggta aagcattttt
     8641 tgggagagat gctttagcaa attatattat tataggaagt aaacaactag gagtagaaat
     8701 tttttctgtt aaagatggta aagttacact aaatattgaa gaaaaagtta tgaaaaagct
     8761 atgggataat ttctatgtac catatataaa tggatatttt gcttcttatg gtaagtttcg
     8821 ttctgatgat gcaaaaacag gtgatatatt agctcttgta ggctctagtt caggagcagc
     8881 atactttcca gatagagtaa tgctaaatga taatgagagt tataatattg aaacatctgt
     8941 ttttcaagca ccacgtttta aaggagaagg aaaatatgct gtacaacaag gtgcaggaat
     9001 ggctgtaaca aaatctacta aagaagaaga atatgcatca gtagaatttt taaaatggtt
     9061 tacagataaa gagcctaata taaaattctc tattgcttct ggatatttac cagtaaagaa
     9121 aaaaacgaat gatttgaatg ttataaaaga agttatgaag aaagataatt tgagtttatc
     9181 tgataaagtt gaaggttcaa tagcctcatc tattgaacaa attaacagtt gtaatttata
     9241 cactaataaa gcatttgatg gtggaactaa agctagacaa atacttgaat attcaatgct
     9301 tgataaagct aaagaagatt caaataaaat aaatgaattg gtaaaaagtg gaacttctaa
     9361 gaaggatgcg gtagcacaat ttaataatga taagaatttt gaaacttggt ttgaaagttt
     9421 taaggctgag cttgaaaatg ctataaaaaa atagatgaat tggcagttag tgtgtagaca
     9481 agcacaaagg tataggtgga ataagtatgg aaaacaatct aagaaaaaac aattcaataa
     9541 tgaaaaagat gatatttccc ctaattattg ttatgctaat tcaaacaagt ttattttgtg
     9601 ccacaatttt atggggagga actattaaga aattaaatga taattcgttt gacatcttaa
     9661 atgaaagagt aattaatcgt aaaaattata tacaaaatga aatgttacaa agatggtcaa
     9721 atacaagtga aacggaagct acaattaatt cctctgtaaa aaaaatatta aatgaaaaaa
     9781 atgccttaat tgaggatgta ggcaacaatg aagaaattaa caaagatatt ataaaaagca
     9841 tatcaaatga tttaatatat cttttgagaa aaaactctgt tacaggagct tttataattc
     9901 taaatgacaa aaatattaac gataagccaa tagatagtaa aggtgaaata agtaggactg
     9961 gattgtatat aagggattta gaccctaagt ttaatccaaa tgataattct gatttgttaa
    10021 ttgagagagg ttcatcagat atagcaagaa gttttggaat atctatggac agttattggg
    10081 aacctaaatt ttcatttaaa agtgaaaatg agcaagaggg agatgatttt ttttataaac
    10141 catttagagc tgcaattgat aatccaaacg tatcaagttc taatttggga tactggggaa
    10201 gtaagttttt attgacaaat gcaggtgata ggcttatgac atatacagtt ccccttattt
    10261 atgaagatgg aactgtatat ggagtacttg gaatagattt aagtgtaaat tatttaagag
    10321 aattacttcc atactcagag ataaatggaa ataaaaaagg taattatatt ttaggtgtag
    10381 atagaaataa tgatatggcc tttgaaaatg taatttcaaa tggatactca ttaaaagaaa
    10441 aattttggga tggtttacat tctaatttag agcagaagga atcgtataaa aatatatata
    10501 agattaaaaa taaagataat gaatgtggta atggcaacat ttatgctagt gtacaatact
    10561 tagatttata taactccaat actccatttg aaaatgaacg ttgggctctt ttgggtatta
    10621 ttgaagaaaa aagtcttttg tcattttcaa aaaggataga gaaattagtt ataatatcta
    10681 tagcaatttc tttattaata ggtctaatag gtatttacat tgcatcaaga ttatttacaa
    10741 aaccaattgt tcttttagcc aacaaagtta gaaacagtaa tccatcaaaa ccagtaaatc
    10801 ttgataagat aaatatatct gaaatagatg aattatcttc agctatagag ttactcagtg
    10861 caaatgttgc agattcttca tcaaaattat cacagattat tgaaatggta gatatgccaa
    10921 tcggggcttt tgagttatac aaaaacgcag agaatgtttt ttgtacaaaa gataaaaaac
    10981 ttaagtggat aagaatgaat atctttgaag atgagctaaa ggttcttggt gttgttgttg
    11041 atgtcacaaa agaatttatt gagaaaagga agatagaata tgaaagagat tttgatattt
    11101 taacgaatct tttaaatagg agagcttttc attcacagat acaagaaaaa tttaagaata
    11161 aagatgaact taagatagca gcatttatta tgtgggattt agataatcta aaatatatta
    11221 atgatacata tggacatgat gctggtgatg aatatattag gtgtgctgca gacatactta
    11281 agaagtttat atcatggaat ggtatagtat ctagactttc aggtgatgag ttttatgtat
    11341 ttatatatgg atatgaaagt aaagatagta taagagatat tattaattct gttaagaaac
    11401 agatgaataa taccttatta aaacttcatg atggaacaga atttaaaata agggcatcag
    11461 caggaatttc ctggtaccct gatgattcaa ccaactatga tgaattaatg agatacgcag
    11521 attttgctat gtatcaaatt aaaaatacaa tcaaggggga agttagtgaa tttgatatta
    11581 atatatataa taaggattca ttcttattga gtaatagaga agaattaaac aagttaattg
    11641 atggtcaatt agttgagtat gcatttcagc ctattgtaga ttgtaaaggt aaagtatttg
    11701 catatgaggc acttatgaga cctaaaataa aaacttttag ttcaccaata gatgtaatta
    11761 gattagctcg ttcacagtct aaattatatc aaattgagcg tattacatgg tttaaagcac
    11821 ttaaatcatt taaaaaatac aaagaaaaat ttggcgattg caaactgttc ttaaattcta
    11881 ttcctaataa taatttatca aaagtagatt ttgaggaaat tgaagattta tatggtgaat
    11941 atttaaatag agtagtatta gaaataacag agaatgataa gattaatgaa gattttattg
    12001 agaaaaagaa agcatatata gaccattgga atatagaaat tgctttagat gactttggga
    12061 caggttacaa cggagatgct gtactattat atataatgcc agactatgta aagattgata
    12121 tgtctattgt aagaggtatt gataaagatg aaaacagaca gaaaatatta aaaaatctta
    12181 tctcatattc taacgaaaga aatattaaag tgatatcaga aggtgtagaa acaaaaggtg
    12241 aaatggaaac actgattaat cttggagtag attatatgca gggttactat attagtaaac
    12301 catcatttat acctgaagat ataagtgaca aaattaaaaa agagctcaag atatgtcatg
    12361 aaaataggaa tgatgtggtt taatagagga aaaattaaat ttatttttat gaacttttag
    12421 tttataatta aaaagaaaat aatataatta taaattaagg tggtggagct aataatcaca
    12481 acaaacatag atataagaaa gttaaatgaa aatcaattag aagcagttga acatatagat
    12541 gggccttgca tgatacttgc agggcctggt tcaggtaaga ccagagtaat tacatataga
    12601 atagccaata tggttgtaaa caaaaatata gcaccaacaa gaatcttggc aataagtttt
    12661 accaaggcat cttcaataga aatgaaaaat agagctttga atttaagtga tgatataaga
    12721 cttaataagg taacctatgg tacttttcac tctgtatttt ttaaaattct aagatacttt
    12781 gaaagataca atttagacag tatatttgat gaaaagtcaa agcgaatgac aatcaaggca
    12841 atcctaaaaa gtttaaatat agagaatgct gatgatgatg agaatatagg acaagttata
    12901 aatgaaatct cctatgtaaa aaatgaactt atggataaaa atgaatttaa ttcagaagta
    12961 ttaacaaaag atgagttttt aaaagtatac aacttatacg aagaacaaaa atctaaagtt
    13021 aacaaaatag attttgatga tatgcttata agaacttatt atttactttt gaacaataag
    13081 tctgcactag aaatggttag aaatgtgtat aaatatatat tgatagatga atttcaagat
    13141 ataaataaag tacagtttga agttttaaag ttaatttgta gcccattaaa caatatattt
    13201 gcagttggag atgaagacca aagtatatat ggatttagag gtgctagacc agattttttg
    13261 cttgaatttg aacaatactt taataatact aaaaaaataa tattagatat aaattatagg
    13321 tctaaaagtg agatagtgta tactgcaaat agacttatcg acaaaaataa aaatagatat
    13381 gaaaaagtca ttaagtgtag tcagggggat ggaggaagtg ttacctacat ttctccacat
    13441 gattcagagg aagaagcctt atatattgcc aaggaaataa ttgacgaaat acaaaaagat
    13501 tatgtagagt attcagattt tgcagtaatt tatagaacaa atatacagtc aagagcatta
    13561 gtagatgtat ttatggatat gaggattcca tttgtagtaa aagactcagt aattacaata
    13621 tatgaccatt gggcaagtca agatatacta gcttatttaa gaataggtat taatccaaaa
    13681 tcaaataaag attggttgag aattataaac aagcctttta gatatatatc caaagacagt
    13741 ataaatatgg ttaaggatga aaaagacttt ataacagcat taataaataa gtgtaatctt
    13801 catcctaaac aagttaaaac aataaatgat ttggaaatag atttaagtta tttaaatact
    13861 ttaaatccta aaaatgcaat atcttatatt agaactagtc ttgattatga tagatacgtt
    13921 ttagattatt gtagcaacag aaagataaaa actaatggac ttgtagagat attaaatgaa
    13981 cttgagagtt cagcaactaa ttttaatact gttacagagt ttttagagca tattgataga
    14041 gtaaagactg agttaacgga aaataacaag aataagcaga ctgagggtgt catatttaca
    14101 actatgcata gtgcaaaagg attagaattt agaaatgttt atattatagg tgtaaatgaa
    14161 ggaacaatac ctcatgaaaa atcatatgat atttgtaagg aagagaaaaa agaagaacaa
    14221 ttagaagaag aaagaagact tatgtatgtt gctattacaa gagctgaaga aaagttatgt
    14281 ataagctcta ctttaaataa gtatggtaag aaagttgata aatcattatt tattaatgat
    14341 ataaaatctc caacgaaaaa agaaattgac agtattggtg taggggataa ggtataccat
    14401 aaaaaatttc atgaaggaga aataattaaa aaagatggaa ttatgtttac aataaagttt
    14461 aaagatagag agcgaatctt ggatttgaaa acttgtttat tgaagaatat aatttatact
    14521 atttaattta gaatctaaat ttagatagaa atataatgta ttatttatta tgtgcaagac
    14581 aaatagatat gtgtaaaatg tattatttaa cttattagaa aaagaaataa ggatttaata
    14641 tattatttat agttgtttag aaaaagcaac aatgtggaaa atattattta gtattattaa
    14701 agaaaagcaa caatgtgaaa gatattattt agtgtaattt aagaaaaagc aacaatgtga
    14761 aagatattat ttagtattat taaagaaaga gcaacagtgt gaaagatact atttagtatt
    14821 attaaagaaa aagcaacagt gtgaaagata ttatttagtg taatttaaga aaagcaacaa
    14881 tgtgaaagat attatttagt attgtttaag aaaaagcaac aatgtgaaag atattattta
    14941 gtattattta agaaatagct aaaaatatga aatatactat ttataattac aaaaataata
    15001 tcaataaata taaacagtag tatttagata atttacaagt aaaaattatt aaaaaaataa
    15061 aaaacttatt gacaaaaata ttataaggaa gtacaattgt attaacaatt taaggaaaca
    15121 aatccaatga tagagaaagt agcatatatg gatttacagc gagttagggg ttggtgtaag
    15181 cctagcaatg aagtctatgt gaagagagct ctggaggaga ttatcagaaa ctttagtatg
    15241 ataattggtt tacccgcctt aagggattga gaggataagt acttacttat caaaaagagt
    15301 ggtaacgcgg atataattcg tctcttagct gtaaagctaa gggacttttt tgatttaaaa
    15361 ataaaaatat ccatacagct taaatgctaa cagtcacaaa ttttgaaagt aatataagta
    15421 tttatcaata agagtggtaa cgcggtaaag tcgtctctta gtcattaaag actgagagac
    15481 tttttttgta actcgaaaca tttaagtatt aaaatctgag ggaaattata attaaaagag
    15541 aggggtagtt aaaatgaaag tatttaaaaa attattaagt ttaggattag tattaggatt
    15601 gacattatcg ctagtaggat gtagtggtgg aggagaaaaa acaaagttag aacaaataaa
    15661 agacaatgga aaattagttg ttggtactag tgcagaattt cctccatttg aatttcataa
    15721 ggtagtagat ggaaaagatt caataaaagg attcgatata atgttggctg aagaatttgc
    15781 aaaagagctt ggtgtaaagg ttgaaattaa ggatatgtca tttgatggat taataggtgc
    15841 tttaaatgca gaccaagttg atattgttct tgcaggtatg tctccaacac cagagagaga
    15901 aaagagtgtt gatttttcag aattatatta tttaagtaga aatgcagtta tagttaaaga
    15961 tgcagatata gacaaagtaa aaactgaaga tgatttaaag aaacttagag ttggagttca
    16021 agcaggaagt atccaagaag aatatgtagt taacacttta aaaatgacaa ctacaaaatc
    16081 tttaaaagca atacctgatt taattacaga attaaaaaat ggaaacatag atgcagttgt
    16141 tactaatgaa gcagtttctt taataaatgt taaaaaatat gatggaataa aaatggcaaa
    16201 tactgaagtt ggtaaagatg taacagaagg tatggcagca gcaatcaaaa aatctgacaa
    16261 taacaaagat tttatagaat tattaaacaa aaagataaaa gaattacaag atggtaagaa
    16321 gatagaagaa tttttaaatg aagcatctac tgaagctgct tcaaattagt aagaaagtta
    16381 tgtgggagat gaaattatga gtttagattt tagtttttta agtagatttg ggacttcttt
    16441 tttggaagga acaggtgtga cagtatcaat ttctcttgtg gcattatgct ttggatttat
    16501 aataggtata attatctgta tggcaaaaat atcaaagagc aaagtattaa gagcaatatc
    16561 atcaatctat attgaggttt taagagggac accattatta gtacagatat atatagtatg
    16621 gtttggttta cctcaattag gaataagatt ccctatgttg tttggtatac caagtgaatt
    16681 tatagctagt gcatttgcat tatcagtaaa ctctggtgca tacgtggcag agatacttag
    16741 atcaggtata caatcagttg ataatggaca aatggaagca agtagatcat taggattaaa
    16801 ttattggtca actatgagat atataataat acctcaagct ataaaaaata tattaccttc
    16861 attagcaaat gagtttatta cacttgtaaa agaatcttca ataatatctg taataggagt
    16921 tgttgagata atgcgtactg ctgatatagt aaaaaatgca gcatttagag cattagagcc
    16981 attaatagta gcagcagcaa tttactttgt tataacattt actctatcaa gattagttgg
    17041 attattagaa aagaaactat ctgtgtctaa ctagatgaga gcatgtttag catttaggga
    17101 aaacatagaa tagtaaaaag gaaagtgata atatgattac aataaaaaat ttaagtaaat
    17161 cttttggaga tttaaatgta ttaaaaaata tagacttaga aatcgctaaa ggcgaaataa
    17221 tggttatagt aggtccaagt ggttctggta aaagtacact tcttagatgt atgaatttac
    17281 tagaaatacc tacaggtgga gaaataatat ttgaaggaaa aaatttggtc gataaaaaaa
    17341 ctaatattga tgaggttaga caaaatatag gtatggtgtt tcaaaacttt aatttattcc
    17401 cacataagac tattttagat aatataacat tagcaccaat taaattaaaa aagatgacta
    17461 aggaagaagc agaaaaaaaa gcagaaattt tgttgagtag agttggtctt ttagataaaa
    17521 aagattcata tccatcacaa ttatctggag gacaaaagca aagaatagca atagctagag
    17581 cacttgctat ggaaccagat atgatgttgt ttgatgagcc aacatctgct cttgacccag
    17641 aaatggtaaa cgaagttttg gatgttataa aagaacttgc taaagaaggt atgacaatgg
    17701 caattgtaac tcatgagatg ggatttgcta aggaagttgc agatagagtt atttttatag
    17761 atggaggaag tatacttgaa gataatactc cagaagaagt tttcggaaat ccaaaacatg
    17821 aaagaacaaa agcattttta gcaaaagtgc tataatatat aaaaaggatg ttattgatat
    17881 tagaaattca atacatcctt ttttagtgat ggttaatatt aattttaaaa ttaaagttat
    17941 attaatatta actttatttt tacgattttt tgcagaaaaa aaaagagctc ggggtaagct
    18001 ctttttttcc accgaattaa tttccgttac taatccgatt tttaataaat ataaaatata
    18061 tccttatgaa agggtattgg gaatagatat attaactaac tagggttacc agggggataa
    18121 ccttaattta ttataacaaa atgcgacaca aaaatcaata ttttttttga aataaattat
    18181 aaaagttatt tttataacaa gtaagcttat aaatttaact tttatagtaa aaataagtat
    18241 atgtataata taatatattt attttatata atatattatt tta
//
LOCUS       DAEPLF010000059        18227 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEPLF010000059 DAEPLF010000000
VERSION     DAEPLF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18227
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.059"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            385..1176
                     /locus_tag="KR314_001749"
     CDS             385..1176
                     /locus_tag="KR314_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBH0328135.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            1205..2425
                     /locus_tag="KR314_001750"
     CDS             1205..2425
                     /locus_tag="KR314_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBH0328136.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2445..3140
                     /gene="pxpB"
                     /locus_tag="KR314_001751"
     CDS             2445..3140
                     /gene="pxpB"
                     /locus_tag="KR314_001751"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBH0328137.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            3170..4156
                     /locus_tag="KR314_001752"
     CDS             3170..4156
                     /locus_tag="KR314_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBH0328138.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRSKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLNSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4375..4941)
                     /locus_tag="KR314_001753"
     CDS             complement(4375..4941)
                     /locus_tag="KR314_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0328139.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            5293..6360
                     /locus_tag="KR314_001754"
     CDS             5293..6360
                     /locus_tag="KR314_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBH0328140.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEESKDISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            6379..7539
                     /locus_tag="KR314_001755"
     CDS             6379..7539
                     /locus_tag="KR314_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBH0328141.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEETELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            7552..9648
                     /locus_tag="KR314_001756"
     CDS             7552..9648
                     /locus_tag="KR314_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBH0328142.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGFNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTKEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(9807..10151)
                     /locus_tag="KR314_001757"
     CDS             complement(9807..10151)
                     /locus_tag="KR314_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBH0328143.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            10443..11333
                     /locus_tag="KR314_001758"
     CDS             10443..11333
                     /locus_tag="KR314_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBH0328144.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            11551..12837
                     /locus_tag="KR314_001759"
     CDS             11551..12837
                     /locus_tag="KR314_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBH0328145.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDICEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKKFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(12926..13369)
                     /locus_tag="KR314_001760"
     CDS             complement(12926..13369)
                     /locus_tag="KR314_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBH0328146.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(13393..14187)
                     /locus_tag="KR314_001761"
     CDS             complement(13393..14187)
                     /locus_tag="KR314_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBH0328147.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(14753..15229)
                     /locus_tag="KR314_001762"
     CDS             complement(14753..15229)
                     /locus_tag="KR314_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH0328148.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPNVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(15330..16499)
                     /locus_tag="KR314_001763"
     CDS             complement(15330..16499)
                     /locus_tag="KR314_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0328149.1"
                     /translation="MLNIRTLCHEINDWIINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKNYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            16662..17525
                     /locus_tag="KR314_001764"
     CDS             16662..17525
                     /locus_tag="KR314_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBH0328150.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDDLYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            17596..18189
                     /locus_tag="KR314_001765"
     CDS             17596..18189
                     /locus_tag="KR314_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBH0328151.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
BASE COUNT         6987 a         2104 c         3112 g         6024 t
ORIGIN      
        1 acttacaaag aatgataatt atatagatga aagaaacaaa taaatgaaga aaaatgattc
       61 tgtcttgcat ataaagtgat taaaaatgaa aaatacttta aaaatgaagg ttaaggccct
      121 tattttaagt gaaaataatt aattattaac taaaaaatgg taaaaagatt ttttgaaaaa
      181 gaaatacaaa tgaatgttca attaaaataa ttgcaaaaat attccttaaa tggtaaaatt
      241 atattaatta aatgcaatag aatagttgca aaaataaaat tgaatacaag tgataaaata
      301 tttcatgcaa tatataaaat aaacaaaatt taaaaattaa aactacttat tctatcaaaa
      361 tgttagcaag gagggggtca taaattgtat aaagtagatt taaatagtga tttaggagaa
      421 agttttggaa cgtataagat tggtttagat gaagaagttt taaaatatat ttcatcagct
      481 aatatagctt gtgggtttca tgcaggtgac cctagtcata tggaaaaaac agtacagcta
      541 gctaaaaaaa atggagtgaa aataggcgca catccaggtt tcctagattt aataggattt
      601 ggaagacgtg agatgaaaat aacaaagcag gaagctaaag attatacaaa atatcaattg
      661 ggtgctttaa tggcatttgc aagttcaaat ggatgtaata tacaacatgt aaaaccacat
      721 ggagcattat acaatatggc agctaaagat aaagagttag ctatggggat ttgtgaagct
      781 atatatgaag tcgataaaga tattatactg ttggggctat ataatagtga aatgataaat
      841 tctgctaaag aaattggact taggtttgca aatgaagtat ttgcagatag agcttatgat
      901 aataatggtt ttttggttcc aagaagtgta gaaggagcag ttatacatga tacaaaacat
      961 gcaatagata gggttgttag gatggtaaaa gaaggaactg tagaaacttt aacaggtgaa
     1021 gttatacata tcaaagcaga ttctatatgt gtacatgggg acaatccaaa agcaatagag
     1081 tttgtcaaag aaataagaaa gcgttttgaa ttagaaggta tagaagtttg tccattagaa
     1141 aacatagaag tttgttcgtc agagaacata gtataagact gataacatga gggggagata
     1201 aaatatgagt acaaaaaata ttacagataa aactaaaaat aaaaaagatg tcggagcact
     1261 tataggggct gcatttataa tggcaacatc agcaattgga ccagggtttt taactcagac
     1321 tgcacaattt acccaagatt ttggaccaaa tttttcattt gtaattttaa ttacaacaat
     1381 cctttttata ggggctcaag taaatgtatg gagagtaatt ggtgtatcag gacttagagg
     1441 tcaggatata gcaaataaaa taataccggg attaggttat ttagtagctt ttttagtagc
     1501 tcttggagga ttagctttta atataggaaa tgttggtgga gctgcacttg gtatgaatgt
     1561 aatgtttaat atgaatatga cattaggaac tgtattaagt ggattaatag ctatatttgt
     1621 atttatgtca aaaaattcaa attctctagt cgataagata actaaatttt tagcattagg
     1681 aatgataatt atagttggat atgtagcaat atcaaatcat cctcctgttg gagaggcagt
     1741 atcaagaatg gtaaaaccag aaaatccaaa aggattgata ttcccaataa taactctact
     1801 tggaggaagt gttggaggtt atataacttt tgctggagga catagattaa tagacggtgg
     1861 aataacagga gaagaaaata taaaagaaat aactaaatct tctcttttgg gtatattagt
     1921 tgccacaatg atgagagttc ttttattttt agcaatactg gcagtggttt caaaaggtct
     1981 tcaacttgac ccagaaaatc cagctgcttc agcttttaaa tttagtgcag gagcaatagg
     2041 atataaattc tttggacttg tattatggtc tgctgcaatt acatcagtta taggagctgc
     2101 ttatacatca gtttctttct taaagacact aaatcctttt atagataaat atgaaaaata
     2161 ttttataata gcatttatag caatatcaac acttataatg gcatttatag gaaaaccagc
     2221 tactttatta attttagcag gagctttaaa tggattgata cttccaatca cattaggaat
     2281 tatgcttata gcatctaaaa gaaaagatat tgttggagat tacaagcatc ctacatggct
     2341 tttaatattt ggattaatag tagttttaat atctgcatat acaggaataa cttctcttag
     2401 cagcctaggt gctttatttg cttaatttag aagaggtgac atttatggaa actagatact
     2461 tgatatcagg ggataaggca gtggttgctg aatttggaaa tgagatatca gaagatataa
     2521 acaaaaaagt aatttcattt atgagagcaa tagaaatatc gaatcttaaa ggtattgtaa
     2581 cagaaatggt accaacatat aggtcactta tgataagtta taatccatta gaaatagatt
     2641 ttgatagttt gattgagaat ttaaaaaaaa tagaagataa tttggaaagt atagtattac
     2701 caaaacctaa aatacatgag attccagtat gttatgatga ggtttttggt atagatatta
     2761 aaaacgttgc aagctacaat aatctcactg tagatgaagt tataaaaata cacacaagca
     2821 gagagtactt gatatatatg ctagggttta ctcctggatt tccatatcta ggtggtatgg
     2881 atgaaagaat agctacacct agattagaag tgccaaggac taaaatttat ggtgggagtg
     2941 ttggaatagc aggttcccag actggtgtat atcctattga tagtcctggt ggatggcaaa
     3001 taattggtag gacaccttta aaattatatg atgaaaatag agaagaacag attttgctta
     3061 gagctggaga ctttataaaa tttgttccaa taacattaga tgaatttata gaaatcgaaa
     3121 aaaataactt aagtatttaa atatcgttat agtacaggga gtggtatata tgggattcaa
     3181 agttgaatta ggaggtttcc aaacattaat acaagacaga ggtagagttg gctatggtca
     3241 atatggtgta tctggatgtg gagcaatgga tgagtatgca catagagtag gaaatatatt
     3301 agtaggaaat tctgaagatg aggcttcttt agaagtttta atgttaggtc ctacaattac
     3361 ctttgatgaa tatacgcaaa tagcagtaac tgggggagat ttaggggcta aaataaatgg
     3421 aaaagaaatt caaaactgga ggtcatatca aataaatcca ggtgatgtat tatcatttag
     3481 aggtgtgaaa tcaggtgcta gagcctatgt gtcaatagct ggtggaatag atgttccact
     3541 tgcaatggga agtaagtcaa cttatacaag atctaaaatt ggcggatttg aaggtagagc
     3601 acttaaaaaa ggagattata taaatacttt tattcaggaa aaagatttta caataaataa
     3661 aaaattaaat tcaaaatata ttccaactta tagttcagaa atcgtactaa gaattgtaaa
     3721 aggtcctcaa tttgatgctt tttctaacgg tgaagtagag aaatttttat caaataaata
     3781 caaagttacg aatgaaatag atagaatggg ttgtagacta gatggagaga gtataaagca
     3841 ccttaatgga gcagatatta tatctgatgg tatttcttat ggagctatac aagtacctgg
     3901 tcatggaaaa ccgattataa tgttatcaga tagacagact agtggtggct atactaagat
     3961 agggaacgtt ataagtgtag atttgtataa gttagcacaa gcaaagccaa atgatgtagt
     4021 aaaatttgaa cttgtagata tatatgaagc acatagacta ttgagagagc aagaagataa
     4081 gattcaagat atctacaaga gtatgaaaaa tataagagtt gtaaaggcaa aagtattaaa
     4141 tgatatagca gtttaattta attttaacaa atacaatttt tattataatt tataatataa
     4201 cttacaaata gctatttact atttaccata attaagatgg tttagattag tcaaatagct
     4261 atttttttag tttgagtaaa tattttaaat ttaaataaat acttcaattt taaaataaaa
     4321 gcaatattta aatttattat attatctaca aaaagtaggt gtgttatgtt gagtttattt
     4381 agattttatt ggccaaattg gaggtgatat aactccatat ccacgagcta ttgtatcttt
     4441 acttggattg atgattttat gtggtacatt agcttttata aatgttgagt cacctggatt
     4501 taatctaact accttttcac ccatacaaat atcaaccaca ccactcgtta ccaccataat
     4561 ttcttctgat gcatgataga tataatcttt agcatcctca ctttctggtt taagttcaaa
     4621 ctctacaaat aaaattgaag gtgtaaattc agttgtaggc attggagata ctatctcgta
     4681 aaacatttca ctctttggaa atttcatcat aactctatct tcttttctaa ttactaaatc
     4741 atcagtatta ttgtcatcca taaacatata taaaggagtg tctaaaactt cagcgatttt
     4801 tctcaatgaa gacaaggatg gttcaatagc atctctttct atttgagaga tataacttat
     4861 agataaatct gtcaatttgg aaacatcact taatgttttt ttcttgcttt ttcttatatt
     4921 tctcatcttt tcacctagca taagaacctc caaataataa aattaatata aaatattcgc
     4981 ttaaaacaaa gtaattatag cacaacatag aagtagtggc aataagaata catagtagat
     5041 tttgacaatt aaaatttaat taaatccaaa taaaagtcta tgaaaatgaa ttattgttaa
     5101 attaatataa agaaaataat aataaaaaaa tttaatagaa gagtattaat tgaattaaga
     5161 ttatcacatt atattttgga aaagagtaaa ttttcagtaa attatcttga catatatatt
     5221 gaagtaataa tataatttaa agtataaaat tttaaaaatt gaattataac ttcaaaaaat
     5281 aaggagggta atatgaagat aacagatatc aaatttgaaa agcttagaat taaattaaaa
     5341 aaacctgtag tagtttcatt tggagtaatt gaatatggag aaagtgtaat tttaaaaatt
     5401 gaaactgatg aagggtattg tggatttggc gaagcagcac ctcttgcagc agttacaggt
     5461 gaagttttgg ataatgttct ttcagtattg cttatgttta aaaaagagtt aataggcaaa
     5521 gacccactag atattgaaac catacataca atcatggatg gaattattat aggtaatact
     5581 tctgcaaaag cagctattga catagcttta tatgatatta aaggaaaaat tatgaatgca
     5641 cctctttata aagttcttgg aggatttgat agtaaagtcc aaacggatat tactataagt
     5701 atagataagc cagaaaaaat ggcagtagaa gctttagaaa gagtaaaaga aggttttagg
     5761 atattgaagc ttaaagcagg tataaatcct gaagacgata ttgaagctgt aaaattaatt
     5821 agagaagccg ttggagagag tataagaatt agaattgatg caaatcaagg atggaacgta
     5881 aatagcagta taaatactat caaaaaatta gaagaattta atgttgatgc aattgaacaa
     5941 gcacttccac attgggattt agatggtact gcttacataa gaaataaaag taatacaaaa
     6001 attatgatag atgaatcgtt acattcacca atagatgcaa ttaaggctat aaaaaagaat
     6061 gcagttgata catttaatat taagctgatg aaatcaagtg gaatatatcc tgctataaaa
     6121 atcaacaaca tagctgaggc aagtggagta aattgtatgt taggatgtat gctggaaacg
     6181 agaattggta taacagcagc agcaaattta atagcatcta aaaagaacat aacagaagct
     6241 gaccttgata gttttatgtt ttgtgaagaa tcaaaagata tatctggagg atttgtaatg
     6301 gatggagata ttatgaatct tgtcaataaa ccaggtttag gaatagaagt aaacttatga
     6361 taaatttgga ggttaaatat gaatacactt ttttcaaatc caggtagtat acttgctgta
     6421 atgacatcaa tgattgcatt gggtttttac ttacaaagat acaaagccat taaaagctta
     6481 ggaccagcac ttacaataat cataatggga ataatacttt caaacttaaa agtagttcca
     6541 gtaagtactg agttatatgg aactatttca acatatgcta tcccagtttc tatgactata
     6601 atgttgatga gtgttgactt aaaagaaatg acaaaattat caagagaacc tttaattgca
     6661 atttttgtag ctgtattaac tgtaagtatc atggcatttt tatttggatt agtatttgca
     6721 gaaaaaatat cagaaggttg gaaggttgct ggaatgtttg taggtacata cactggtgga
     6781 agtgctaatc ttacagctat tggaacaggc cttaatgtaa gtagacaaac tcttgctgca
     6841 gcaaatgcag cagactatgt aattggagta ccaacattga tatttatgtt tgctctacca
     6901 gcaatattaa aaaattcaaa gaagtttaaa aaactttggc catatcatgt agaagaaaca
     6961 gaattagaag attgtcaaaa tgaagaattt atggagtcaa aagaatggag tattaaagat
     7021 atagcttgga tgttggctat aggttttgtt gttacagaag tttcaacaat acttgctgga
     7081 tatttcaatt caagttttag tagtgcagca agaatacttt tagttacaac aatttcaatt
     7141 ataattgctc aattaaagcc agtaaagaaa ctaaagggaa atttagattt aggattattt
     7201 gttgcattat tctttttatg tacaataggt ttttcagtag atataaaaga gtttttaggt
     7261 tcaactttta ctataacact ttactgtttt agcataattt ttgcttcatt tgtttttcat
     7321 ttaggcataa ctagactttt aaagataaag tatcaatatg taatactttc tatagtagga
     7381 gcaattgctg atggtcctac ttcagcttta gtagctgctt ctgcaaagtg gaattcgctt
     7441 gtaagtgtag cagtcgtaat gggagtaatt ggtggagtat taggaaatta tgcaggtata
     7501 tcagtggcat atgcaataaa gatgtctcta ggattatagg ggggagttta attgataaaa
     7561 tttaaattat acatatctgg actatacagt ggaaatgtaa tctttgatgg cgatttatta
     7621 attgaaaagt tgaatccttt tacaaataaa atagaaagtt taaaaccaat aagcaaagaa
     7681 gaaaatacat actatttaaa tcttacaaaa atagatttaa agtctttatt taataacttt
     7741 gatgtatata ctaaaagctt agtaaacaca gataaagaca cagttataaa taacttagga
     7801 gaaacgcatt ctaaacttaa tgaatatata tgggttcaaa gaaacaaaaa gttcccacta
     7861 gatataatta ttgtagataa taaaatagta ggttttatct gtttatccag agaaacttgt
     7921 actatattaa ttatggatgg atatgaagag tatactgtat taaaagaatg ggaaaaaacc
     7981 cataaaaatg aagagatata ttctataaga tttggtggaa attatatgat tgatatgaaa
     8041 gatggtataa aactttctac agatgtatat ctccctgatt ttgtagattc taccaaaaaa
     8101 gcaccaacaa ttcttatgag aactccatat ggaaaagaaa atgataaaga aatatattat
     8161 aaatatgtac aaagaggata tgctgttgta atacaagatg ttaggggaag aaatgaatca
     8221 gaaggtaagt gggaacctat gattcatgag agggaagatg gagatagtac cataaattgg
     8281 atagtatcac aggaatggtc aagtggaata gttggaatgc ttggagcatc atatcttggt
     8341 tatgtccaat gggcagctgc atcttcaggc aataagcatc ttaaagcttt agttagtata
     8401 gtaacatcag gaagcccttt tattgatata ccaagaaaag gaggagcttt tgtatctggt
     8461 atgcttgcat gggcatttat ggtatcaaga aataaagttg atagaagtaa aatggtaaga
     8521 gatgattggg atgatgtttt aaacatacga ccaatagaaa atatacctgt tgaagctctt
     8581 ggatatagaa ttgaatttct ggaagaatgg ttaaagagag tagaaaaaga tgagtattgg
     8641 gatttgatgg attggcattt acaaaaagat aaaataaatg tacctgcatt ggttgtatct
     8701 gggtggtatg atgataatag tatgggaact acagaggcct tagatgttat aaaggactat
     8761 gaaaaaggaa agagaaaggc tattctaggt ccatggatgc ataattcaaa tactttaaga
     8821 gatataaatg gtatttcttt aggaaatagt tcattaagat atgatttgga ttataattat
     8881 ctattatggt ttgataaata cttaaaaggt atagaaaata acatagatac gactgctcca
     8941 gtagagtatt actctgtagg ttttaataag tggaagacag aagaaaattg gccaataata
     9001 aataagatag ataaaagtat gtatctaata agtgatggaa atgctaacac atctttagga
     9061 aatggtagat tagtatttga taatgattta gaagaaaagt atgatagtta tatatataat
     9121 ccaaaagacc catcagtaca actaattgat atgtcagaga atgaagttgg agttccaaat
     9181 aactacaaag atttagaaaa aagaagtgat atgttgtgtt atacatcaga tgcttttagt
     9241 gaagaattta ctgtaacagg tgatataaag ttagagtttt ttgcatcaag ttctgccaaa
     9301 gatactgact gggttataaa aattatggac gtggatttag atggaaattc aattaaatta
     9361 gctgatggta tacttagtgc tagatttaga aatagttttt ataagtcaga gtttatggaa
     9421 gaaggagaga tttataagtt tacgattata acttcaaaaa tatcaaacac gtttaaagtt
     9481 ggtcataaaa ttagactaga tataacgtct agtgctaaaa actttatttt ccaaaatagc
     9541 aacacgaaag agggatataa tagtattgaa tatatagaag caaagaatac gatttaccat
     9601 ggaggtaaat atccatctaa attgatattg ccaatagaaa ataagtaaat ttatatagat
     9661 taaatagtga ggaggtgtct caaaatgaat tttttagttc gtgagacact ttcttttata
     9721 cgaaatcaaa tatattttca tcatttcaac aatgaaaaag aggcttatct ctctgttttg
     9781 agatagcctc ctataagcta aattgattat tttgtagctg caacaacgct catttcaact
     9841 aacaaatgct ctctagcaag tctagcttca acacaagctc tagcaggttc aaatcctttt
     9901 tcaacccaag catcccaaac agagttcata gcttcaaagt ctttcatatc tcttaaataa
     9961 atagtaacag atagcaaatg ttgcttatct gaaccatatt tgtttaataa atcttctatt
    10021 ttagctaaaa cttcagttgt ttgttcaatt actcctcctt cagcatgagt ttgtccacat
    10081 aaataaactg tattgttatg aactacagct ctactcattc ttccagttcc ttcatatctc
    10141 tttatttcca tattttaaat acctccatag ttaagaaatc tattaaaaat ctaaataata
    10201 aattaaaaaa agtctatact atataaaatt atattatata ttctgataat aatccataaa
    10261 aacatataac tttttagcta taattattat tgttattaat tgaaaagact tattagttac
    10321 ttgacatagt ttacactttt aattatagta taaattggaa tatttagata attattattt
    10381 aatgtataag ctaagttttt aattaatatg gctttataca tttatataga aaagaggtac
    10441 gtatgagtag cacaatcaaa tcaaatcgaa taaaaggtat aatgtttata atagcatcag
    10501 cgcttggctt tgcaatgatg tctgcattcg taaagctagc tggagattta ccaagttttc
    10561 aaaaagtgtt ctttagaaat ctggtatcag ctataatagc tttttggctt atcataaaac
    10621 ataaaggaag tctaactggt aaaaaagaaa atttaaaagt acttttatat aggtcaatat
    10681 ttggaactct aggagttata tttaactact atgcaattga caagttgatg ctttcagatg
    10741 ctaatatgct aaataaaatg agtcccttcc ttgtagtaat attttgtgca attgctctaa
    10801 aagaaaaaat aaatgtaaag cagataggag ctattataat tgcatttata ggagctttat
    10861 tcataataaa accaacattt agtgtagaag taattccata tcttggggga attgcaggag
    10921 ctgtatttgc agcaatggca tatacttgtt taagagttct tggagataga gaagattact
    10981 atacaattgt atttttcttt tcagtttttt cattagttac tgtagggcca attgcttttg
    11041 cagtatatga accaatgacc cttatgcaac ttatatatct attactaggt ggagtatttg
    11101 caagtctagg tcaatttgga ataactttag catataaata tgcacctgct aaggaaatct
    11161 ctatatttga ctatagtaat ataatctttt cagcaatttt aagtatattc ttattcaatg
    11221 tctatccaga tatgttaagt gttgttggat atttaatagt attttcggca gcattttata
    11281 tgtttttata caataagaag ttagataaat tagacaaaga aaagaagaag taaactagat
    11341 aaaagtgttt ttataatttg tattgaatat ttaagtcttt gtattaaaat taaagaaaat
    11401 ttaattaaaa atgtttggta tattaactta taacagcagt taaaattgaa aaattataac
    11461 tgctgtttct tttattaaaa atatccaaat gtctgtaaaa atagactata atagagtagt
    11521 ataaataata taaaggggat aaattaaagt atggaaaaag tgaaagttgc tgtgattgga
    11581 gcaggtaata gaggaacgta tgcatatgct ccatatattt atgaaaattc tgatatatgt
    11641 gaaatagttg ctgtagcaga acctaaaaaa ggaagacgag aactatttac acaaaaatat
    11701 aacctagata gtaaaaatgt atttgaaact ttagaagact tttttaaaca tgacaagatg
    11761 gcagatgcag taataatagc tacaaatgat gatagacatt atgatgtagc aaaactagct
    11821 cttgaaaaag gttatcatgt actattagaa aagccaatgt ctaatagttt agatggtttg
    11881 gtacatatag atgacttgtg tgataagtat aaagataaga tttttatgat ttgccatgta
    11941 ctgagatatt ctccatttta taataaatta aaagaaattg tagaaagcaa aaaattagga
    12001 gagctagtta gtatacaata taatgaaaat ataggatatt ggcattttgc acatagcttt
    12061 actagaggga attggagaaa tagcaatgaa accagccctt taattttagc aaagagttgt
    12121 catgacatgg atattttgtt atatttagta ggaagtagat gcaaaaaaat atctgcattt
    12181 ggaagcttaa agcatcttaa taaccaaaat gctagtggag agatggcaca aaattgtctt
    12241 cagtgtttag ttgaaaaaaa atgtccatat tcagcaaaga gaatttattt agaaaaagat
    12301 agaagtataa atagagctgt tcacataaat ccaactgaag agaatctatt aaatatactt
    12361 aagacaagtc catatggaag atgtgtttac agatgtgata ataatgttgt agacaatatg
    12421 gtaaatatat tacagtttga aaatggagtg acggctactt ttaatttatg tgcatttact
    12481 aaagaaaatg gaagaactat aaagttaatg tttagtcatg gtgaagttgg tggggattta
    12541 aataaaaatg agataagaat taaaaaattt ggaaaaaacg aagaaatagt tatgaatcca
    12601 tcaaatcaac agaatatggt agaggaatat gatagaaatc ttatagctga atttattaaa
    12661 ttagtttcaa ataaagaact agaaaagggt agagtagcag caaaagaagc tatacaaagt
    12721 catgtaatgg catttgcagc agaatattct agggtcagtg atgaagtagt gtatatagaa
    12781 gaattttttg atagtgctaa gcaaatgact aaggagatag aagaaactat attttaatta
    12841 ataaaaattt attaaataaa ataaagatag attaaataat agaaatacga aaatatgtgt
    12901 tctattaagg aatctatctt ttttattaag gttttttctt atccctatca aaaaaatcca
    12961 tacattctga taaaagataa gaaagtaaag cgaataagat ttctgtactt aaaggaatcg
    13021 ttactgaatc cataaaggta tcaattatgt tcatagctag aaaagttagt ataaaatcaa
    13081 ttataaattc gcaaagttta tatattgagt atggtaattt gtttacatat ctgaatatat
    13141 ctaaaatagt agtgcataga aaatcaattg gagtggttat acaaaaatat attaaaaaga
    13201 aaattaatac agctccaaga gatttatatt caaatcctaa gaatgataag aagccaattc
    13261 ctataaccgt gaaaatacaa aaagttatac caaaaaaaac agcaaaaaat aaaaatacag
    13321 aaataaattc gcctaaagga atccttcttt tattacttat cttaatcaac tccttaattt
    13381 atgatatgaa atttatataa ataataacat tcttaaaaat tcataatagt cctctgtttc
    13441 aaatagtact gtttgagaat ctattttttt catatttgga taaaaagagg ccttaaaagc
    13501 tttataatgt tctctaaatt ttatttctat cttaaattta tctggcaatt ttattaagtg
    13561 cctagataaa tctccagata aagctttttt gacacctttt tcaatttttt ttagtgcaag
    13621 gtttggatgg attgaaattg aaccatttcc aattcctttt gacacaggaa cagtgactat
    13681 atttggattt agtttttttg cactctcaca aagcatttca tctccactta aaaatgctac
    13741 aggaacatta taatatgcag caatataagc atttatagta aattcagatg ctatttcatc
    13801 atttatttta aaatagtcat aatcaatatc catagtatga gaaagtggat taccattaca
    13861 agtagctcca gagtgatagc cggtgaatat acatgcatca aagctttcat caattccagc
    13921 catcataact agtgggtctt tggtccatcc tctaagtatt ttgacgtttt caggtaaaat
    13981 atttggatta atatttctac cactgtcatg tgcatcttta acaagtattt catgtacacc
    14041 agattgagta gctccatcac atgctgctgt tatctcttta ctcatttgtg ttctatagtg
    14101 ttgtgaaaat tcacattcaa gttttgtttc atcccaattt acaataccac aagtaccttc
    14161 tatatcagca cttaaataca atttcataat atataccccc ttatgtttat tattgatgat
    14221 aataacagat aacagaaaat acataatcaa taaagggcat aaaaatatta tgtcttacta
    14281 ttaattatta ttaaatctgt aactgaaaaa taactaaatt ggtattaaaa aatataacac
    14341 tatgtgtcat ttatttatat agttttttat atctgatgtt tatataaaat tgtaacatat
    14401 aagtggttac atttactagt ttaacatagt taaaatagtt aggtattatt tttaagttaa
    14461 attgattatt ataaaatcgt agaataaaag aaaaatttaa tttattaaaa tgcatgaaat
    14521 gttttttcct caacataatg ctaaattaaa ctcaaaaaga aataatagaa aaatcttatg
    14581 tagcttagaa agaatgtgaa tgatttaagg gaaaaataga aatttattat ataatattag
    14641 gctgtattat gtaatacagc ctaatattat atgtatatgt gacataaaat cttggtaagt
    14701 gtaaataaaa taaatgtgga aaacaaatta atgaaatcaa gaaaaaagta atttaaaatt
    14761 caatttcatc ttcccaatta aattctcctg atttttctac actcttaggc cagtgactac
    14821 accatttagg aacggacgga ttttcaaccc tatcaacgct tggagtatat ggctttaagt
    14881 ctataacagg actattatca ttagcatcaa tatatggtat ttttattata ccattttcat
    14941 ggtcgatatt gataatttgt acagcagtca atgctattgg attgggtctt ataggtgacc
    15001 ttgttgcaaa tattcctata acgtttggtc cacttttata aggttttggg gtttctaaaa
    15061 tacttcttag ttcaggatta tcaaacccat cagcccacca aaatacattt aaatgactga
    15121 aatcgtcgag ttcatttaat gcaggtacat actttttgtc taatttaata aagacctctt
    15181 cgccttgaat acatatttta cctataggat ttattaatat ttctttcata atattacctc
    15241 caactattta atttatcatt tgtaatttta gggtataaca tgacatcatg tgagggtcaa
    15301 tataaaataa taaataaaaa tattaatagt tagaatgata atacattttt tacatggagc
    15361 atgacaccag taataaggca atcttcgtca atatcaaaag tagaggtatg caaaggggaa
    15421 gtaatgtttt tttcacgatt tgcacaacca aggtggtaaa aagctccttc acagtgtctt
    15481 agaaaatacg aaaagtcttc agccccaaga gtgggcgagt ctttaaggat aatattttct
    15541 tctccaaaca attctacagc attttgcttg acgtaatcta ctagatggtt tgaattgata
    15601 agagctggat aaccttcctc aatttcaaca gaaatacttc ctccaaatgt tttacaagta
    15661 aaatcgcaaa tttctctaat tcgttttttt gaaaactctc tagtttcagg agtcaaggtt
    15721 ctcaaagtac cccttataac cactttgtca caaactatat tttccttgat tccaccttca
    15781 atctttccta gagaaataac cacagaatca gttggatttg tgtttcgact aacgattgtc
    15841 tgtagagctg taataatatg agcagatata acaatagcat caataccttg atgtggataa
    15901 gctccatgac attttgaacc atgtacagtt aattgaagtg tatcggtgga agcatttaga
    15961 gtgttgtatt ttaattcaat tagatttttg ttgatattgg gatttacatg gagtccaaat
    16021 atatatttta catttgggtt ttcaagacat ccatcttcta tcataagttg agccccacca
    16081 atagtttcct cggcaggttg aaaaaagaat tttacattta catttaagta attctttaat
    16141 ttaaatagta tattacaagt acccagtaaa atagcagtgt gtgcatcatg accacatgca
    16201 tgcattttac caatattaat agatttataa gaataattaa gttcttctgt tataggtaag
    16261 gcatctatat cagctcttat agcaactgtt tcaaagtttg gactcacgtt aatgtatgca
    16321 gttatccctg tatgattctt atattcaata taatttatac ctatttcatt taagtattta
    16381 ataatttttt tcttagtttg aaattcttct aatccaagtt ctggagtctt atgtaaatct
    16441 cttcttatat ttataatcca atcatttatc tcatgacaaa gagttcttat attaagcata
    16501 aaacacatcc taactatatt aaaaatttat ttttcttcat taattatatt attttatgta
    16561 aatcattatt atgtatcaag tacaaataaa atatcttata attatattag agatatttag
    16621 tatggagaag aatatatcat tttataagaa gaggtgttta aatgttagga ataattgcgc
    16681 catctgggcc attaagaaat acaagtttgg aggaaataaa gtttaattta gaaagttatg
    16741 gttacgaagt taagttttct gaaagttgta gtttaaatta taaaggttat ttagctggta
    16801 atgatgatat tagagcaagg gatatagaag atatgttttt agataaagat gtagatataa
    16861 ttatgtgctt aagaggaggt tatggaacaa caagaatatt ggataaaata aattatgata
    16921 taataaagca aaatccaaaa ccatttatag gtttttctga tataacaggt ttaaatttag
    16981 ctttttataa aaattgtgga cttttaccat atcatggaat aatggcagca aatgtaggta
    17041 aatgggataa atttacctat aaatcattgg taaatgcttt agaatttaaa gatgacttat
    17101 atttagaaaa tccaaaagaa gaaaagattt atactgtatg tgaaggtaaa gcagagggaa
    17161 taataatggg aggaaattta tccttaataa ttgccacaat gggaacaaaa tatgaaatag
    17221 atgcaaaaga taaaattttg tttattgagg aaattggaga acctcaatat aaactggata
    17281 gaatgttaac acaactatat tcttcaggaa aacttgagga atgtaatggt ataatttttg
    17341 gagattttaa ggattgtatt gaagaaaatg atttgatgga attactaata gaatttgcaa
    17401 ataaggtcaa taaaccttct atatataatc ttcagtcagg acattgtatc ccaatgataa
    17461 ctattccttt gggcagaatg tgcgaattag atgctacaga aaaaatcgtt aaattaaaaa
    17521 aatagtgtgt atataaatat aatagaaata tggtataatc ttatacaagt aaaaatttag
    17581 ctaggaggat gaaaaatgtc tgatgataga ataatagact ttaacgaact aaagaacaaa
    17641 gttaaagatt ctgatgtaga taaatttgag caatacatat ataacttata tttttctgtt
    17701 atggatggaa agatgtccat ggctgagttt tcaagaaaaa tattcgatta tatgagagat
    17761 aacaacatat ctcaagaaaa atttatgaaa atacaaaagc aatttatgga gagatatggt
    17821 atggacactg aagaagtaga aaaacaactt agaaactttg gaatcgaccc ttctacagca
    17881 ggttttatga gtaataatac ttccagcaaa gtatctacag aagatttaga gtcatttaag
    17941 aagagtgcag gattttatga gaaatatgga gaaaaaatcc aaccaaagag ttgtataaca
    18001 acatttataa aaaatgactt aaatgatatt aatgtaataa tcgaccaaga aaagataatg
    18061 ctttgtagtg atagaaaaat aaatcttatg gattcagaac taaatgagtt tttattagaa
    18121 tataagaata tgtttaataa aaaaataaag gtagtaatgt gcgaaactac gaataagtat
    18181 gattattaaa aaatgaattt aaatactgaa gtagtatcaa ttaattg
//
LOCUS       DAEPLF010000060        18039 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEPLF010000060 DAEPLF010000000
VERSION     DAEPLF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18039
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.060"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            251..985
                     /locus_tag="KR314_001766"
     CDS             251..985
                     /locus_tag="KR314_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0328152.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMVLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELTPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRDIIGTLKNLIIE"
     gene            2127..3383
                     /locus_tag="KR314_001767"
     CDS             2127..3383
                     /locus_tag="KR314_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBH0328153.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFLLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGIAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKTLGLEKFS
                     FALSSKVVFDLPIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            3446..4639
                     /locus_tag="KR314_001768"
     CDS             3446..4639
                     /locus_tag="KR314_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBH0328154.1"
                     /translation="MLTSLALIFLLGMASGGIFEKIKLPSLLGMLLTGIILGSYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMNIGIFLQIKYRVVARRLSVKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGIASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     EGKSK"
     gene            4846..5226
                     /locus_tag="KR314_001769"
     CDS             4846..5226
                     /locus_tag="KR314_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0328155.1"
                     /translation="MYLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKILSKLSKKRFLYVQETGKCMYYTVAIKEKTYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYI"
     gene            6135..6689
                     /gene="tcdR"
                     /locus_tag="KR314_001770"
     CDS             6135..6689
                     /gene="tcdR"
                     /locus_tag="KR314_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBH0328156.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            7020..14120
                     /gene="tcdB"
                     /locus_tag="KR314_001771"
                     /gene_synonym="toxB"
     CDS             7020..14120
                     /gene="tcdB"
                     /locus_tag="KR314_001771"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBH0328157.1"
                     /translation="MSLVNRKQLEKMANVRFRVQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIVESATNDTLESFREN
                     LNDPRFDYNKFYRKRMEIIYDKQKNFINYYKTQREENPDLIIDDIVKIYLSNEYSKDI
                     DELNSYIEESLNKVTENSGNDVRNFEEFKGGESFKLYEQELVERWNLAAASDILRISA
                     LKEVGGVYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMVKLEAIMKYKEYIPGYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNAFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKKNYFEGSLGEDDNLDFSQNTVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGLDVDSLSTEIETAIDLA
                     KEDISPKSIEINLLGCNMFSYSVNVEETYPGKLLLRVKDKVSELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKIIVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLAECEINVISNIDTQVVEGRIEEAKSLTSDSINYIK
                     NEFKLIESISDALYDLKQQNELEESHFISFEDILETDEGFSIRFIDKETGESIFVETE
                     KAIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDATHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     VIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTAATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELILRDKATKVVDYFSHISLAESEGAFTSLDDKIMMP
                     QDDLVISEIDFNNNSITLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPVITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMNINIELNENDTWVIDVDNVVRDVTIESDKIKKGDLIENILSKLSIE
                     DNKIILDNHEINFSGTLNGGNGFVSLTFSILEGINAVIEVDLLSKSYKVLISGELKTL
                     MANSNSVQQKIDYIGLNSELQKNIPYSFMDDKGKENGFINCSTKEGLFVSELSDVVLI
                     SKVYMDNSKPLFGYCSNDLKDVKVITKDDVIILTGYYLKDDIKISLSFTIQDENTIKL
                     NGVYLDENGVAEILKFMNKKGSTNTSDSLMSFLESMNIKSIFINSLQSNTKLILDTNF
                     IISGTTSIGQFEFICDKDNNIQPYFIKFNTLETKYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVIISPNIYTDEINITPIYEANNTYPEVIVLDTNYIS
                     EKINININDLSIRYVWSNDGSDFILMSTDEENKVSQVKIRFTNVFKGNTISDKISFNF
                     SDKQDVSINKVISTFTPSYYVEGLLNYDLGLISLYNEKFYINNFGMMVSGLVYINDSL
                     YYFKPPIKNLITGFTTIGDDKYYFNPDNGGAASVGETIIDGKNYYFSQNGVLQTGVFS
                     TEDGFKYFAPADTLDENLEGEAIDFTGKLTIDENVYYFGDNYRAAIEWQTLDDEVYYF
                     STDTGRAFKGLNQIGDDKFYFNSDGIMQKGFVNINDKTFYFDDSGVMKSGYTEIDGKY
                     FYFAENGEMQIGVFNTADGFKYFAHHDEDLGNEEGEALSYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGISIINDGKYYFNDSGIMQIGFVTINNEVFYFS
                     DSGIVESGMQNIDDNYFYIDENGLVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFDPETKKAYKGINVIDDIKYYFDEN
                     GIMRTGLITFEDNHYYFNEDGIMQYGYLNIEDKTFYFSEDGIMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            14242..14742
                     /gene="tcdE"
                     /locus_tag="KR314_001772"
     CDS             14242..14742
                     /gene="tcdE"
                     /locus_tag="KR314_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBH0328158.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAVLLDAMTDEM
                     NAKDEK"
     gene            14821..14982
                     /locus_tag="KR314_001773"
     CDS             14821..14982
                     /locus_tag="KR314_001773"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328159.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYMDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            15071..15253
                     /locus_tag="KR314_001774"
     CDS             15071..15253
                     /locus_tag="KR314_001774"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328160.1"
                     /translation="MRDEGQNDRENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            15470..>18039
                     /gene="tcdA"
                     /locus_tag="KR314_001775"
                     /gene_synonym="toxA"
     CDS             15470..>18039
                     /gene="tcdA"
                     /locus_tag="KR314_001775"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBH0328161.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     LLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTIPRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKDSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     "
BASE COUNT         7167 a         1836 c         2920 g         6116 t
ORIGIN      
        1 gaattatcaa aaataaaata gttattagaa aaaaataaaa gtaattgcaa agattgcaat
       61 tacttacttc tatattaatt actataccat aaaataacaa aaaaatcaag tcgagtaatc
      121 cttcaactta agtgatatac taggtattaa taatttttaa ttaataaaat ataactagag
      181 gaaaatctac attttctcaa tttctttaat taaattgacc tctaattaag taattataag
      241 gagaaaaatt atgaatttag caaagggatt agatgtttta aaaatttcat caaatgtatt
      301 cggtgaagat aaagttatgt atattcctgt aatctacaca gaagatgatg caacacttat
      361 tgacacaggt cttccgggtc aaggtgattt aataattgat gctcttaata aaagtaacac
      421 gagctttgat aggttaaaaa atataataat tactcatcat gatatagacc atataggaaa
      481 cataaattat ctaagagaaa aatctaagaa taatatcaaa gtatatgcat acaaaagtga
      541 agtcagttat ataacaggag aagaaactcc ttttaaactt tatatgttgg aacaaatggt
      601 agataaaatt gatgataaga tgcttagtat gctaaatgtt atggtacttg gatttaaatc
      661 atcatatact aaagtagatg tgtcattaga taaccatgaa aaattgaatc taggtgagga
      721 aatagaagtt atacatacag gaggtcacac tagaggtcac atatgtcttt atttaaaaga
      781 gtctaaagta cttattgctg gagatttgtt acaggtagaa aatggagaac ttacgcctgt
      841 tgatgtaatg cacagcaaca aacaagagtt aaaagatgct attaaaaata taagtaatta
      901 tgatatagaa acgatagttt ttagtcatgg tggattatat caaagagaca taattggaac
      961 attgaaaaac ctaattattg agtagtacta tatgatttac aataatttct taaattactt
     1021 aaaattatat ctaaagatgt agaatcaaaa ttaaatataa gtaataagaa ttttgcttta
     1081 aaattatact taagtgttaa gtatgatacg tagtattaca agtattttag aatataattt
     1141 tttatagtaa ttttaaggtt ctacaatact tcagttgaaa aatatttaac agcatagtat
     1201 aataggaaag aaaatatttt aaataaactt tagtatacaa agtgaagaaa aatatttaaa
     1261 ttatatataa tattgtagag tgaggtaaaa tatttaagtt atatatcata atacagagtg
     1321 aagaaaaata tttagataat gtaccataat acaaggtgag taaaaatatt taaagcatgt
     1381 ataataatgc aaagcgaaga aaaatattta aataatgtac cataatgcag agtgagtaaa
     1441 aatatttaaa taatgtgtta taatacaaag cgaataagat tatttaaagc atgtattata
     1501 atataaagtg agtaaaaata tttaaatcat gtatcataat ataagcagag tgaagaaaaa
     1561 tatttaaata atatatcata atgtagagtg aggtaaaata tttaagttat gtatcataat
     1621 acaaagtaaa gaaaatcatt taaattatat atcataaaaa taatatgaaa tattgaacac
     1681 tcataatatt ggaggcttta atattttaat tgataggtta cattatttat aacattaaaa
     1741 atacaattta cataaaatag acatatgaaa aataaaaaat tatataataa ttatgggaat
     1801 gaggttctcc cttggatgat aatatcctaa actgccaatg agctgatgac ttctatgata
     1861 tcatagatgc caccagcttt ttttgttgtt taaatcgata aaatggaggt gaattactta
     1921 ctaaattttt attttttaaa tttgaagaaa tatttaatta atagaaaaaa cagtagatta
     1981 tatgttaatt attttattaa gaatgggtta agagtatacc tatatcctat caatttaaaa
     2041 aacaattatg atagtcatta cattgactac agttgatgca ataaaacttt caaaaaatct
     2101 tatgatgcaa atagaaagga attaaaatga tagaaaaatt taaaacttta atatggatta
     2161 aaatgataat aagaattaaa agcgtttata aaacatatgg tggactattt ttgttaggac
     2221 tcataggtat accagttggt gcaattatag gtcttataga caccatattt ggaacggttc
     2281 ttctaaaagt tacagatatt cgagaaacat atccaatgta tctgatacca tttttagcag
     2341 tagtaggagt tgttattgct tattgctatt ttaaatttgg aggaaaaagt agtaaaggaa
     2401 tgaatctaat atttgaggta gggcatggag aagaagaaat tattccttta agattggttc
     2461 catttataat atcaggtaca tggttaaccc atttatttgg tggtagtgca ggacgagaag
     2521 gagttgctgt tcagattggt gcaacttttt cacattgggt aggtaaaagg ttgcctataa
     2581 aaaatgcttc tagtattttt ctagtaactg gtatagcagc tggttttgca ggattatttg
     2641 agacaccaat agcagcaata ctatttgcta tggaggtttt ggtagctggt tcactggagt
     2701 atcaatcgct ttttcctgcg tttactgctt cttttacagc aagtgcagta tcaaaaactt
     2761 tagggttaga gaaattttct tttgcacttt cttctaaagt tgtatttgac ttaccaatat
     2821 tttggaagct tattgtttta ggaattattt ttggaatggt tggtggagca tttgcatggt
     2881 gcctgaaatt gtcaaaaaga aaaataggta atagattaaa aaatcctatg ataaggattg
     2941 ctattattgg agtatgtctt agtgtattat tccttttatt ttataaagga agatattcag
     3001 gtttagggac aaatttaatt caaaatagtt tttatggtgg agaaatttat tcatttgact
     3061 ggctgttaaa gtttatactt acaatactga ctttgtcagc aggttttcaa ggaggagagg
     3121 ttacaccttt gttttccatt ggagctagtc ttggtgtatt gttagcagga ttttttaatt
     3181 tgccaattga attagttgca gcgcttggct atgctagcgt ctttggcagt gctacaaata
     3241 ctttttttgc tcctgtattt ataggggcag aagtttttgg atacagctat ttaccttatt
     3301 tctttgtagt atgcgctatt tcatatatat ttaatatgga taaatctatt tattcactac
     3361 aaaaaatttc aacgaaacaa taaataaaac aacataatgt atgattataa gagaaagaat
     3421 attagagaaa ggatagggta ggaatatgtt gacaagttta gctttgattt ttttattagg
     3481 aatggcctcg ggaggtattt ttgagaagat aaaattacct agcttattgg gaatgttact
     3541 tacaggaatc atattgggtt catatgtttt aaatttgatt gataattcaa tactagatat
     3601 ttcttctgac cttagaaaga ttgcattgat tattatatta acaagggctg gattatcttt
     3661 agatataaac gatttgaaga aagtgggaag acctgctgta cttatgtgct ttattccagc
     3721 tacttttgaa attatcggaa tgatagtttt agcaccaaaa ttactaggcg tttctatatt
     3781 ggaagcagca gtcatgggtg cagtagtagg agcagtttct cccgcaatta ttgtacctaa
     3841 aatgcttaaa ttaatggaag aaggttatgg tacagaaaaa agtattccac agatgctttt
     3901 agcaggaact tctatagacg atattttcgt aattgtgatg tttacagtat tcacaggatt
     3961 ggcacaagga aatagtatat cagctattag ttttttacaa attccagtat caattatatt
     4021 aggtgtaata gttggagcag tgattggaat ctgtttagca gtatttttta agaaggttca
     4081 tatgagagat tctgctaaag gggtgttgct tttaagtatc tcatttttaa tgataagttt
     4141 ggagactgct ttagagggta ttgtgccatt ttctggatta ttagcagtta tgaatattgg
     4201 gattttcctt cagataaaat atcgtgtagt tgcaagaaga ttgtctgtaa agtattcaaa
     4261 actatgggtt ggtgctgaaa ttctcttgtt tgttttggta ggggcaacag tagatataag
     4321 ttatgcattt aaagcaggaa ttggagctgt aattttaata tttggagttc ttttattccg
     4381 tatggtagga gtattcttct gccttattaa gacaaatttg acaataaaag aaagattgtt
     4441 ctgtatgatt ggctatattc caaaggctac agtacaggca gcaataggag gtgtaccact
     4501 tgcaatgggg atagcatctg gacaacttat tttgacatta gcggttctcg ctatacttat
     4561 aactgcacct cttggagctt ttggaattga tgtaacttat aaaaaattat taacttctgt
     4621 agaaggtaag agtaagtaag aataaaaata aagattattt taagtgataa attatatttt
     4681 ggaaaaaact atattattga gtacaatgtt agaaaatcat ttgagtgatt acagaaggta
     4741 gaagtttata tcggatttta aggcatgtga tgattattag aattgatttt gtgaaattgt
     4801 tattgtaata tttaattata tagataaaga agcggagtga taactatgta tttaaaaaaa
     4861 ttatcaaaat tagagttagt tattatgaaa tttatttgga atttagatat aaaaaccaat
     4921 tcatatgaga ttattgatta tatgaaagaa gagcataatt taccagaaaa agtggcttta
     4981 aaaattttat caaaattatc aaagaaaaga ttcttatatg ttcaagaaac aggcaagtgt
     5041 atgtattata cagttgcaat aaaagaaaag acatatttag agtttatatc aagaaatgtg
     5101 caaaatcttt tgaagaataa ttttataagg aatttattag tatcatttca cgaagaggag
     5161 ctaacagagg aaaagataaa gtcgttagaa aactgggtag taaattggga agaagcttat
     5221 atataaatgt tattattttt attacaattt agataatttt ggttttgtaa gctctgcaac
     5281 tatttttaga tggttgcaga gcttattttt attttttatt cccttaagca atacttaaaa
     5341 gtaaacgcat ttatatttac taacttcaat cacttctaat acttagatta ctttttaaaa
     5401 atattttgat atgtcatctc tatagtaatc tagaacttgt ttctattctt tatataaagt
     5461 ttttatgaat gatatatata atattcgttc atttgattaa aaataacaaa atattaaata
     5521 attctactct gatagtttgt taaaaaaata ataaaaaata ttaataaaca aaaaaaaaaa
     5581 ttatcttaag agaggagaat gttctaaaat ataaaaaggt ttctagattt cataaaagat
     5641 actattttag tcttgaaaat atttagtttg aaaagatttt aatttaatga ttgattaagt
     5701 taaaaatgtg catgtaataa attttatttt atttattgac taaattataa agtttacata
     5761 attatttaat aattatgtaa ttgttactta aaaattgatc tattttaaaa tctagttata
     5821 acttcgaaaa agactgaaaa ttaagaaaaa agaaatataa gtataaaaat atatcatata
     5881 gatttttttt attttacttt aataaaatga tttgttttta caatacttta ttaatataaa
     5941 gtttattgct aaaatacttt atttattaga aaaaaattac taattaatta ttaaaattaa
     6001 tgtatttata atgcgtattt tcatataaaa tttaatttat ttgccgatta tataattata
     6061 atgactgatt taattccaat gttgtcaaaa tttttaaata aatcatcatt ataaatataa
     6121 gagaggatga ttttatgcaa aagtcttttt atgaattaat tgttttagca agaaataact
     6181 cagtagatga tttgcaagaa attttattta tgtttaagcc attagtaaaa aaacttagta
     6241 gagttttaca ttatgaagag ggagaaacag atttaataat attttttatt gaattaataa
     6301 aaaatattaa attaagtagc ttttcagaaa aaagcgatgc tattatagtc aaatatattc
     6361 ataaatcatt actgaataag acttttgagt tgtctagaag atattctaaa atgaagttta
     6421 attttgtaga atttgatgaa aatatcttaa atatgaaaaa taattatcaa agtaagtctg
     6481 tttttgagga agatatttgt tttttcgaat atattttgaa agaattatct ggtattcaaa
     6541 gaaaagttat tttttataaa tatttaaaag gatattctga tagagaaata tcagtgaaat
     6601 taaaaatatc tagacaagct gttaataagg ctaaaaatag agcatttaaa aaaataaaaa
     6661 aagactatga aaattatttt aacttgtaat taatgaattt aaagaaatat ttacaataga
     6721 aatcaaattt tagaattaac tttattgtaa aatcaataac ttaatctaag aatatcttaa
     6781 tttttatatt ttatatagaa caaagtttac atatttattt cagacaacgt ctttattcaa
     6841 tcgaagagca aattaatcaa ctgagtgtct tcaatttaaa atgttaggaa gtgaatgtat
     6901 atgaaaacct aagtagatat tagtatattt tataaataga aaggaggata tataaaagag
     6961 ttttagcatt tagatgtaaa aatattcaat aaaaatatta tagtaaagga gaaaatttta
     7021 tgagtttagt taatagaaaa cagttagaaa aaatggcaaa tgtaagattt cgtgttcagg
     7081 aagatgaata tgtagcaata ttggatgctt tagaagaata tcataatatg tcagagaata
     7141 ctgtagtcga aaaatattta aaattaaaag atataaatag tttaacagat atttatatag
     7201 atacatataa aaagtctggt agaaataaag ccttaaaaaa atttaaggaa tacctagtta
     7261 cagaagtatt agagctaaag aataataatt taactccagt tgagaaaaat ttacattttg
     7321 tttggattgg aggtcaaata aatgacactg ctattaatta tataaatcaa tggaaagatg
     7381 taaatagtga ttataatgtt aatgtttttt atgatagtaa tgcatttttg ataaacacat
     7441 tgaaaaaaac tatagtagaa tcagcaacaa atgatacact tgaatcattt agagaaaact
     7501 taaatgaccc tagatttgac tataataaat tttacagaaa acgtatggag ataatctatg
     7561 ataagcagaa aaatttcata aattactata aaactcaaag agaagaaaat cctgacctta
     7621 taattgatga tattgtaaag atatatcttt caaacgagta ttcaaaggat atagatgaac
     7681 ttaattccta tattgaagag tcattaaata aagttacaga aaatagtggg aatgatgtta
     7741 gaaactttga agaatttaaa ggtggagagt cattcaaatt atatgaacaa gagttggtag
     7801 aaagatggaa tttggcagct gcttctgaca tattaagaat atctgcctta aaagaagttg
     7861 gtggtgtata tttagatgtt gatatgttac caggaataca accagaccta tttgagtcta
     7921 tagagaaacc tagttcagta acagtggatt tttgggaaat ggtaaaatta gaagctataa
     7981 tgaaatacaa agaatacata ccaggatata cttcagagca ttttgatatg ttggatgaag
     8041 aagttcaaag tagttttgaa tctgttctag cttctaagtc agataagtca gaaatattct
     8101 catcacttgg cgatatggag gcatcaccac tagaagttaa aattgcattt aatagtaaag
     8161 gtattataaa tcaaggacta atttctgtga aagactcata ttgtagcaat ttaatagtaa
     8221 aacaaatcga gaacagatat aagatattga ataatagttt aaatccagct attagcgagg
     8281 ataacgattt caatactaca acgaatgctt ttattgatag tataatggct gaagctaatg
     8341 cagataatgg tagatttatg atggaactag gaaagtattt aagagttggt ttcttcccag
     8401 atgttaaaac tactattaac ttaagtggcc ctgaagcata tgcggcagct tatcaagatt
     8461 tattaatgtt taaagaaggt agtatgaata tccatttgat agaagctgac ttaagaaact
     8521 ttgaaatctc taaaactaat atttctcaat caactgaaca agaaatggct agcttatggt
     8581 catttgacga tgcaagagct aaagctcaat ttgaagaata caaaaaaaat tactttgaag
     8641 gttctcttgg agaagatgac aatcttgact tttctcaaaa tacagtagtt gacaaggagt
     8701 atcttttaga aaaaatatct tcattagcaa gaagttcaga gagaggatat atacactata
     8761 ttgttcagtt acaaggagat aaaattagtt atgaagcagc atgtaactta tttgcaaaga
     8821 ctccttatga tagtgtactg tttcagaaaa atatagaaga ttcagaaatt gcatattatt
     8881 ataatcctgg agatggtgaa atacaagaaa tagacaagta taaaattcca agtataattt
     8941 ctgatagacc taagattaaa ttaacattta ttggtcatgg taaagatgaa tttaatactg
     9001 atatatttgc aggtcttgat gtagattcat tatccacaga aatagaaaca gcaatagatt
     9061 tagctaaaga ggatatttct cctaagtcaa tagaaataaa cttactggga tgtaacatgt
     9121 ttagctattc tgtaaatgta gaggagactt atcctgggaa attattactt agagttaaag
     9181 ataaagtatc agaattaatg ccatctataa gtcaagactc tattatagta agtgcaaatc
     9241 aatatgaagt tagaataaat agtgaaggaa gaagagaatt attggatcat tctggtgaat
     9301 ggataaataa agaagaaagt attataaagg atatttcatc aaaagaatat atatcattta
     9361 atcctaaaga aaataaaatt atagtaaaat ctaaaaattt acctgaatta tctacattat
     9421 tacaagaaat tagaaacaat tctaattcaa gtgatattga actagaagaa aaagtaatgt
     9481 tagcagaatg tgagataaat gttatttcaa atatagatac acaagtggta gaaggaagga
     9541 ttgaagaagc taaaagctta acttctgact ctattaatta tataaagaat gaatttaaac
     9601 taatagaatc tatttctgat gcactatacg atttaaaaca acagaatgaa ttagaagagt
     9661 ctcattttat atcttttgag gatatattgg agactgatga aggctttagt ataagattta
     9721 ttgataaaga aactggagaa tctatatttg tagaaactga aaaggcaata ttctctgaat
     9781 atgctaatca tataactgaa gagatttcta agataaaagg tactatattt gatactgtaa
     9841 atggtaagtt agtaaaaaaa gtaaatttag atgctacaca tgaagtgaat actttaaatg
     9901 ctgcattttt tatacaatca ttaatagaat ataatagttc taaagaatct cttagtaatt
     9961 taagtgtagc aatgaaagtt caagtttatg ctcaattatt tagtactggt ttaaatacta
    10021 ttacagatgc agccaaagtt gttgaattag tatcaactgc attagatgaa actatagatt
    10081 tacttcctac attatctgaa ggattacctg taattgcaac tatcatagat ggtgtaagtt
    10141 taggtgcagc aatcaaagag ctaagtgaaa caagtgaccc attattaaga caagaaatag
    10201 aagctaagat aggtataatg gcagtaaatt taacagcagc tacaactgca atcattactt
    10261 catctttagg aatagctagt ggatttagta tacttttagt tcctttagca ggaatttcag
    10321 caggtatacc aagtttagta aacaatgagc ttatcctaag agataaagca acaaaagttg
    10381 tagattattt tagtcatata tcattagctg agtctgaagg agcatttact tcattagatg
    10441 ataaaataat gatgccacaa gatgatttag taatatctga gatagacttt aataacaatt
    10501 caataacttt aggtaaatgt gaaatctgga gaatggaagg tggctcaggt catactgtaa
    10561 ctgatgatat agatcacttc ttttcagcac catcaataac atatagagag ccacacttat
    10621 ctatatatga cgtattggaa gtacaaaaag aagaacttga tttgtcaaaa gatttaatgg
    10681 tattacctaa tgctccaaat agagtatttg cttgggaaac aggatggaca ccaggtttaa
    10741 gaagcttaga aaatgatggc acaaaactgt tagaccgtat aagagataac tatgaaggtg
    10801 agttttattg gagatatttt gcttttatag ctgatgcttt aataacaaca ttaaaaccaa
    10861 gatatgaaga tactaatata agaataaatt tagatagtaa tactagaagc tttatagtcc
    10921 cagtgataac tacagaatat ataagagaga aattatcata ttctttttat ggttctggag
    10981 gaacttatgc attatctctt tctcaataca atatgaatat aaacatagaa ttaaatgaaa
    11041 atgatacttg ggttatagat gtcgacaatg tcgtaagaga tgtcactata gaatctgata
    11101 aaattaaaaa aggagattta atagagaata ttttatctaa attaagtatt gaagacaata
    11161 aaattatttt agataatcat gaaattaatt tctctggaac attaaatgga ggtaatggat
    11221 ttgtatcttt aacattctca atcttagaag gaataaatgc agttatagaa gttgatttat
    11281 tatctaaatc atataaagtt cttatttctg gtgaactaaa aacattgatg gcaaattcaa
    11341 attctgttca acagaaaata gattatatag gattgaacag cgaattacaa aaaaatatac
    11401 cttatagttt tatggatgat aaaggaaaag aaaatggatt tattaattgt tctacaaaag
    11461 aaggtttatt tgtatctgaa ttatctgatg tagttcttat aagtaaagtt tatatggaca
    11521 atagtaaacc tctatttgga tattgtagta atgatttgaa agatgttaaa gtcataacta
    11581 aagatgacgt tattatatta acaggatatt atttaaaaga tgatataaaa atctctcttt
    11641 cttttactat acaagatgaa aatactataa aattaaatgg agtatattta gatgaaaatg
    11701 gagtagctga aatattgaaa tttatgaata aaaaaggtag tacaaatact tcagattctt
    11761 taatgagctt tttagaaagt atgaatataa aaagtatttt cataaattcc ttacaatcta
    11821 atactaagct tatattagat actaatttta taataagtgg tactacttct attggtcaat
    11881 ttgagtttat ttgtgataaa gataataata tacaaccata tttcattaag tttaatacac
    11941 tagaaactaa atatactcta tatgtaggta atagacaaaa tatgatagta gaaccaaatt
    12001 atgatttaga tgattctgga gatatatctt caactgtcat taatttttct cagaaatacc
    12061 tttatggaat agacagttgt gttaataaag ttataatttc gccaaatata tatacagatg
    12121 aaataaacat aacacctata tatgaagcaa ataatactta tccagaagtg attgtattag
    12181 atacaaatta tataagtgaa aaaatcaata ttaatatcaa tgatttatct atacgatatg
    12241 tatggagtaa tgatggaagt gattttattc ttatgtcaac tgatgaagag aacaaggtat
    12301 cacaagttaa aataagattt actaatgttt ttaaaggtaa tactatatca gataagatat
    12361 cttttaattt tagtgataag caagatgtat ctataaataa agttatttca acatttacac
    12421 cttcatatta tgtggaagga ttacttaatt atgatttagg tctgatttct ttatacaatg
    12481 agaaatttta tattaataac tttggaatga tggtgtctgg attagtatat attaatgatt
    12541 cattatatta tttcaagcca ccaataaaga acttgataac tggatttaca actataggtg
    12601 atgataaata ctactttaat ccagataatg gtggagctgc ttcagtcgga gaaacaataa
    12661 ttgatggcaa aaactactac ttcagccaaa atggagtgtt acaaacaggt gtatttagta
    12721 cagaagatgg atttaaatat tttgctccag cagatacact tgatgaaaat ctagaggggg
    12781 aagcaattga ttttactggc aaactaacta ttgatgaaaa tgtttattat tttggagata
    12841 attatagagc agctatagaa tggcaaacat tagatgatga agtgtactat tttagtacag
    12901 atacaggtag agcttttaaa gggctaaatc aaataggtga tgataaattc tatttcaact
    12961 ctgatggtat tatgcaaaaa ggatttgtta atataaatga taagacattc tattttgatg
    13021 attctggtgt gatgaagtca ggatatactg aaatagatgg aaaatatttt tactttgctg
    13081 agaatggaga aatgcaaata ggagtattta atacagcaga tggatttaaa tattttgctc
    13141 atcatgatga agatttagga aatgaagaag gtgaagcact ttcatattct ggtatactta
    13201 attttaacaa taagatttat tattttgatg attcatttac agcagtagtt ggatggaagg
    13261 atttagaaga tggttcaaaa tattactttg atgaagatac agcagaagca tatataggta
    13321 tctcaataat taatgatggt aaatattatt ttaatgattc tggaatcatg caaattggat
    13381 ttgtcacaat aaataatgaa gtattttatt tctctgattc tggaatagta gaatctggaa
    13441 tgcaaaatat agatgataat tatttctata tagacgaaaa tggtctagtt caaattggtg
    13501 tatttgacac ttcagatgga tataaatact ttgcaccagc taatactgta aatgataata
    13561 tctatggaca agcagttgaa tatagtggtt tagttagagt tggtgaagat gtatattatt
    13621 ttggagaaac atatacaatt gagactggtt ggatatatga tatggaaaat gaaagtgata
    13681 aatattattt cgatccagaa actaaaaaag catataaagg tattaatgta attgatgata
    13741 taaaatacta ttttgatgag aatggaataa tgagaacagg tcttataaca tttgaagata
    13801 atcattacta ttttaatgaa gatggtatta tgcaatatgg ttatctaaat atagaagata
    13861 agacgttcta ctttagtgaa gatggtatta tgcagattgg agtatttaat acaccagatg
    13921 gatttaaata ttttgcacat caaaatactt tagatgagaa ttttgaggga gaatcaataa
    13981 actatactgg ttggttagat ttagatgaaa agagatatta ttttacagat gaatatattg
    14041 cagcaactgg ttcagttatt attgatggtg aggagtatta ttttgatcct gatacagctc
    14101 aattagtgat tagtgaatag ataaaaatac gttaaatata tcctcttata cttaaatata
    14161 taaaaataaa caaaatgata cactacataa agtgttctat ctaatatgaa gatttaccaa
    14221 taaaaaggtg aactatgatg aatgcacagt agttcacctt tttatatttc taatggtaac
    14281 aaaatatttt tttatataaa cctaggaggc gttatgaata tgacaatatc ttttttatca
    14341 gagcatatat ttataaagtt agtaatttta actatatcat ttgatacatt attaggatgt
    14401 ttaagtgcaa taaaaagtcg taaatttaat tctagttttg gaatagatgg aggaatcaga
    14461 aaagtagcaa tgatagcatg tatatttttt ttatcagtag ttgacattct tacaaagttt
    14521 aactttttat ttatgttacc acaagattgt atcaattttt taagactaaa acatcttgga
    14581 atatctgaat ttttctctat tttatttatt ttatatgaaa gtgtaagcat attaaaaaat
    14641 atgtgcttat gtggattacc agtacctaag agattaaagg aaaaaatagc agttttacta
    14701 gatgcaatga cagatgaaat gaatgctaag gatgaaaagt aagtaatggt agatataata
    14761 aaaatattaa caaataaaaa gtgttatcca aataagaata gctgaaagtt atcataattc
    14821 atgaaactga taatgaaaac gagggagcag atgccaagag acacacaagt attaaataca
    14881 tataatttcg aagcaagtgt tcattactat atggatgaca aggtagtata tcaaacattg
    14941 gttcacaaag atggtgcatg gtcagttggt aaaatctatt aagctacatt agttacagat
    15001 atcacaaact ataatagtta aacatagaaa tatgtgtaaa ttgtgatgga aattattcaa
    15061 aaacacaaaa atacgtgatg aaggacaaaa tgatagagaa aataagtatc aaaccttaat
    15121 aaatgattta attgatagtt taaaagttat aggaaaaata tataaagaaa taaaaacatt
    15181 aaaaaaatat aagatatgtt tacaaattac tatcagacaa tctccttatc taatagaaga
    15241 gtcaattaac taattgagta tctttaaatt gaaatgttag gaagtgattt aaatatgaaa
    15301 acttaaatta taaaaaatca atattaattt atctttaaaa aatagaaagg agtgtataag
    15361 atttattttc aaagtttaaa aacaagaaaa tcaatttaaa tttcagaagg aataaatgtg
    15421 gttatagaag tggatttatt atcaaaaata atagtactag gaggttttta tgtctttaat
    15481 atctaaagaa gagttaataa aactcgcata tagcattaga ccaagagaaa atgagtataa
    15541 aactatacta actaatttag acgaatataa taagttaact acaaacaata atgaaaataa
    15601 atatttacaa ttaaaaaaac taaatgaatc aattgatgtt tttatgaata aatataaaaa
    15661 ttcaagcaga aatagagcac tctctaatct aaaaaaagat atattaaaag aagtaattct
    15721 tattaaaaat tccaatacaa gtcctgtaga aaaaaattta cattttgtat ggataggtgg
    15781 agaagtcagt gatattgctc ttgaatacat aaaacaatgg gctgatatta atgcagaata
    15841 taatattaaa ctgtggtatg atagtgaagc attcttagtc aatacactaa aaaaggctat
    15901 agttgaatct tctaccactg aagcattaca gctactagag gaagagattc aaaatcctca
    15961 atttgataat atgaaatttt acaaaaaaag gatggaattt atatatgata gacaaaaaag
    16021 gtttataaat tattataaat ctcaaatcaa taaacctaca gtacctacaa tagatgatat
    16081 tataaagtct catctagtat ctgaatataa tagagatgaa actttattag aatcatatag
    16141 aacaaattct ttgagaaaaa taaatagtaa tcatgggata gatatcaggg ctaatagttt
    16201 gtttacagaa caagagttat taaatattta tagtcaggag ttgttaaatc gtgggaattt
    16261 agctgcagca tctgacatag taagattatt agccctaaaa aattttggcg gagtatattt
    16321 agatgttgat atgcttccag gtattcactc tgatttattt aaaacaatac ctagacctag
    16381 ctctattgga ctagaccgtt gggaaatgat aaaattagag gctattatga agtataaaaa
    16441 atatataaat aattatacat cagaaaactt tgataaactt gatcaacaat taaaagataa
    16501 ttttaaactc attatagaaa gtaaaagtga aaaatctgag atattttcta aattagaaaa
    16561 tttaaatgta tctgatcttg aaattaaaat agctttcgct ttaggcagtg ttataaatca
    16621 agccttgata tcaaaacaag gttcatatct tactaaccta gtaatagaac aagtaaaaaa
    16681 tagatatcaa tttttaaacc aacaccttaa cccagccata gagtctgaca ataacttcac
    16741 agatactact aagatttttc atgattcact atttaattca gctaccgcag aaaactctat
    16801 gtttttaaca aaaatagcac catacttaca agtaggtttt atgccagaag ctcgctccac
    16861 aataagttta agtggtccag gagcttatgc atcagcttac tatgatttca taaatttaca
    16921 agaaaatact atagaaaaaa ctttaaaagc atcagattta atagaattta aattcccaga
    16981 aaataatcta tctcaattga cagaacaaga aataaatagt ctatggagct ttgatcaagc
    17041 aagtgcaaaa tatcaatttg agaaatatgt aagagattat actggtggat ctctttctga
    17101 agacaatggg gtagacttta ataaaaatac tgccctcgac aaaaactatt tattaaataa
    17161 taaaattcca tcaaacaatg tagaagaagc tggaagtaaa aattatgttc attatatcat
    17221 acagttacaa ggagatgata taagttatga agcaacatgc aatttatttt ctaaaaatcc
    17281 taaaaatagt attattatac aacgaaatat gaatgaaagt gcaaaaagtt actttttaag
    17341 tgatgatgga gaatctattt tagaattaaa taaatatagg atacctgaaa gattaaaaaa
    17401 taaggaaaaa gtaaaagtaa cctttattgg acatggtaaa gatgaattca acacaagcga
    17461 atttgctaga ttaagtgtag attcactttc caatgagata agttcatttt tagataccat
    17521 aaaattagat atatcaccta aaaatgtaga agtaaacttg cttggatgta atatgtttag
    17581 ttatgatttt aatgttgaag aaacttatcc tggtaagtta ctattaagta ttatggacaa
    17641 aattacttcc actttacctg atgtaaataa agattctatt actataggag caaatcaata
    17701 tgaagtaaga attaatagtg agggaagaaa agaacttcta gctcactcag gtaaatggat
    17761 aaataaagag gaagctatta tgagcgattt atctagtaaa gaatacattt tttttgattc
    17821 catagataat aagctaaaag caaagtccaa gaatattcca ggtttagcgt caatatcaga
    17881 agatataaaa acattattac ttgatgcaag tgttagtcct gatacaaaat ttattttaaa
    17941 taatcttaag cttaatattg aatcttctat tggtgattac atttattatg aaaaattaga
    18001 acctgttaaa aatataatcc acaattctat agatgattt
//
LOCUS       DAEPLF010000061        17906 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEPLF010000061 DAEPLF010000000
VERSION     DAEPLF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17906
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.061"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..1407
                     /locus_tag="KR314_001776"
     CDS             <1..1407
                     /locus_tag="KR314_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBH0328162.1"
                     /translation="SIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLNDLSRVKYD
                     YRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKALKYITNQNEDI
                     CIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEDQTEDVCLAFIERAS
                     KNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYSALSCVKEQ
                     TEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICLEAVRIDGR
                     ALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKEILSYVKIP
                     TERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEEICLEAVNQSYE
                     ALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVDNQTEKVCL
                     EAVKQNRKALQYVKQKQS"
     gene            1775..2353
                     /locus_tag="KR314_001777"
     CDS             1775..2353
                     /locus_tag="KR314_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBH0328163.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKFVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            2383..2529
                     /locus_tag="KR314_001778"
     CDS             2383..2529
                     /locus_tag="KR314_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBH0328164.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            2834..4213
                     /gene="scfB"
                     /locus_tag="KR314_001779"
     CDS             2834..4213
                     /gene="scfB"
                     /locus_tag="KR314_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBH0328165.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            4242..4739
                     /locus_tag="KR314_001780"
     CDS             4242..4739
                     /locus_tag="KR314_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBH0328166.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            5037..6701
                     /locus_tag="KR314_001781"
     CDS             5037..6701
                     /locus_tag="KR314_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBH0328167.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            6714..9164
                     /gene="recJ"
                     /locus_tag="KR314_001782"
     CDS             6714..9164
                     /gene="recJ"
                     /locus_tag="KR314_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBH0328168.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            9177..9689
                     /locus_tag="KR314_001783"
     CDS             9177..9689
                     /locus_tag="KR314_001783"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBH0328169.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            9763..11970
                     /locus_tag="KR314_001784"
     CDS             9763..11970
                     /locus_tag="KR314_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBH0328170.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            11995..12444
                     /locus_tag="KR314_001785"
     CDS             11995..12444
                     /locus_tag="KR314_001785"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBH0328171.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNASRPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            12459..13073
                     /locus_tag="KR314_001786"
     CDS             12459..13073
                     /locus_tag="KR314_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0328172.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVSDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            13164..14720
                     /locus_tag="KR314_001787"
     CDS             13164..14720
                     /locus_tag="KR314_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBH0328173.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNRLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDKKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEELNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            14710..15972
                     /locus_tag="KR314_001788"
     CDS             14710..15972
                     /locus_tag="KR314_001788"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBH0328174.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            16046..17833
                     /gene="aspS"
                     /locus_tag="KR314_001789"
     CDS             16046..17833
                     /gene="aspS"
                     /locus_tag="KR314_001789"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBH0328175.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTKMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDIQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT         7280 a         1785 c         3355 g         5486 t
ORIGIN      
        1 tctatatttt taccagaaaa aaatgactat ttagatgatg aagaatttga gtatgatata
       61 gaaaataatg aagaagattt aaaggaagat aacaaagtac atttaaatga tttaagcaga
      121 gtaaaatatg attacagaga gttaaaagac atagaaaacc aaacagaaga gatgtgtctg
      181 ctagcagtaa agcaggatgg tactataatt gagtttgtaa aggacaagac ttataaagta
      241 tgtatggaag cagtaaaaca gacatataaa gctcttaagt atataacaaa tcaaaatgag
      301 gatatttgta tagaagcagt aaaacagaac tacagagctt tgtactacat aaataataag
      361 acagaaaatg tactaattga ggctataaaa aatgcatcaa cttatgatgt catggaagta
      421 tttaaatttg tggaagatca aacagaagat gtatgtctag catttataga aagggcatct
      481 aaaaatgatg ttgcagagat attaaaagga ataaaagagc aaactccagc catatgttta
      541 gaagctgtaa aaaaagatgg aaaatcttta gcttatgtaa aggaacaaag taatagtata
      601 tgtttagaag ctgtaaaaga aaactacagt gcactatctt gtgtaaaaga gcaaacagaa
      661 gaaatatgta tagaagcagt aaaacagaat gactttgcat tgtattatgt aaatgaacag
      721 acagaaaaaa tatgtatgga agcagtaaaa agaagttata tggctctaca gtatgtaaat
      781 aagcaaacag aagagatttg tttggaagca gtaagaattg atggaagagc attacagtac
      841 gtaaaagagc aaacagaaga aatttgcttg gagtcagtta gacaaaatgg aaaagtattg
      901 caatatgtaa aaaaacagac tgaaaatata tgtatagaag cagtaagagg ttcatttgaa
      961 gaacttgaaa taaaagaaat attaagttat gtaaagatac caacagaaag aatatttgta
     1021 gaagcagtaa agcaaaatgg aaaaattctt aaatatgtag aaaatcagac agaattaata
     1081 tgtttagagg cagtaagaga aaactataat gctttagctt atgtaaagga acaaacagaa
     1141 gagatttgtt tagaagcagt taatcaaagt tatgaagcgc ttaaatatgt gaaagaacaa
     1201 acagaagaag tttgtctaaa agcagtaaaa caagattata gaatgttgaa atatgtaaat
     1261 aatcaaacag aaaaaatatg tttagaagca gtaaagcaaa attatagagc tttagaattt
     1321 gttgataatc aaacagaaaa agtttgtctt gaagcagtaa aacagaatag aaaagcacta
     1381 cagtatgtaa aacaaaaaca aagttaaaag gctaaaatat atagaaaata tatgcaaata
     1441 aataaaattt gatattttgt aataaataaa atgggtatct caaaatgaac tttttagttt
     1501 gtgaggtact ctttttatat gaaataaaat atatttttag ccttttaaca atggggaaaa
     1561 ggtttatttc tattttgaca taggcttttt atgcatataa agcttgataa aattgtttta
     1621 ttataattgt tttgttatat aaataataat aagaaaatta tataggtctt tttttgacca
     1681 gttttgcatt tttaacaact agttttgtaa agtatattga attttctcaa aaaagaatgt
     1741 actctatata tgtaaaattt ttttggaggt aaaaatgttt aaaagatatg ctgaaaaaat
     1801 gacatctgtt ttaatttgta ataatatgat agataataat gaatctaaag tctattctta
     1861 tggatttgaa atattaatag ctttcatagt taatataacc accatgctgt ttataggttt
     1921 tttatttggt aaatttacat atgtattatt ttttttaatg tgttattgtc caataagaca
     1981 attttcagga gggtatcatg cagacaatta ttttagatgc ttattaacct ttatttttat
     2041 aattttatct acaatcttaa ttatagaaaa tataaatata gacttattta aaaatattat
     2101 tatgattatt gcaagtgtta gttgggttgg aatttgtgtt ctgtgtccaa tagaacatag
     2161 gagtaaccct ataagtgata gggaaaagtt tgtttataag aaaactgcta tatttatatc
     2221 tacagtagtg ttattgataa caattttaag tctttctttg agtatatttg tagattattt
     2281 cacatatagc gcatttgcca tgttttggat ttttgtgatg ttagtattag gtaagttaaa
     2341 agctaaagta taagtaaaat atgtatgagg gagagttaag ttatgaaaaa gtttattgta
     2401 agatttatga agtttgctag ctcattggca cttagcactg ctattttgtc agctaatagt
     2461 acctgtcctt ggattattca tcagcctaaa gtacccaaag aaatcagcaa tctaaagaag
     2521 acaaattaaa tacaagcact tatttataca aaattatcag gaaaaaagaa attattatat
     2581 tttaataaga ttaaaacaat ataaaatcag atacaaaatt tattttattt attttatagg
     2641 agcctaatac ctatttggta atatattagt gcgccaattt agggggatat agtgtattta
     2701 atgaaatgac ttacggagag taagtcattt ttgtattgta tgtattttat gtaaacttat
     2761 gatataatga agggtaaagt ttaattaaaa taataaaaaa ctttagtata gatagtaatt
     2821 taggaggtag ggtatgagta tgatacataa attttctatg aatggatata atatcgtttt
     2881 agatgtgaat ggtggagctg ttcatgtgtt agatgatgtt gcatacgatt tgttagattt
     2941 ttataaagaa aaaagtaaag aagaaatatt agagatatta aaatcaaaat atcaagaaga
     3001 aaaaataaat gaagcatatg aagaaatatt aaatttggaa aaagaaggtc ttttatacac
     3061 agaagataca tatcaatatc atccaagctt tgtacataga gagcctgtag taaaagcttt
     3121 atgtttaaat gtagctcatg attgtaactt aaaatgtaaa tactgttttg cagctcaagg
     3181 tgattttgga ggagaaaaag aacttatgag ttttgaagtt ggtaaggcag caattgacta
     3241 tttgattgct aattcaggaa gtagaaagaa cctagagata gatttctttg gtggagagcc
     3301 tttgatgaac tttgaagttg taaaacaact ggttgactat ggaagaagtg tagaaaaaga
     3361 ttataataaa aatataagat ttacaataac tacaaatggt gtactgttaa atgatgagat
     3421 tatagattat ataaatgaaa atatgcataa tgtagtttta agtttagatg gaagaaaaga
     3481 agttaatgat aatatgagac caactttaaa tgataaagga agttatgata ttacattacc
     3541 acgatttaag aaacttgtgg aaaaaagagc aaaagataag tattattata taagaggtac
     3601 ttttacaaga gataatttag atttttcaaa agatgttatg cattttgcag atttaggatt
     3661 taaattaacc tctgtagagc cagtcgttgg agatgagagt aatccatatg cacttagaga
     3721 agaagattta cctaagatat ttgaagagta cgaaaaattt gctgtagagt atgcagatag
     3781 acaattacaa ggagatggat ttaaattttt ccattttatg atagacctaa atcaaggtcc
     3841 atgtgtaata aagagaataa ctggatgtgg agctggaaat gagtatcttt ctgtaactcc
     3901 aaatggagat atctatccat gtcatcaatt tgttggaaat gaagaattta aaatggcaaa
     3961 tatatttgat gaagaaatag tacttccaga aaatttaaaa aatatgttta gagaagctca
     4021 tgtatatact aaagaagagt gtaaacaatg ttggaataag ttttactgtt caggtggatg
     4081 tcatgcaaat gcgataaact ttaataatga catatcaaaa ccatatgaat taggatgtga
     4141 aatgcaaaga aaacgtacag agtgctctat aatgatacaa gctaagttaa tgttagaagg
     4201 agcaaccaac taaatttata ttttaaagga gttgtttata tatgattaga gattatttag
     4261 aagataaacc tttaattgac gaaagtgtat ttgtggcaaa atcagcagat gtaattggaa
     4321 atgttaaaat aggcaaagat tcaagtatat ggtacaatgc agtagttagg ggtgatgaag
     4381 gaccaataac tattggagaa aacactaata tacaagattg ttctatagtg catggagata
     4441 cagaaactat aattggtaac aatgtaacag ttgggcatag gtctatagtt catggttgta
     4501 aaattagtga taatgtgtta attggtatgg gttcaataat attggataat gctgaaatag
     4561 gagaatatac tctgatagga gcaggtacac taattacatc taataagaag tttccaccag
     4621 gtgttttgat aatgggttct cctggtaagg ttgttagaga gcttacagaa gaagataaaa
     4681 aatatattga tgaatcttat gaatggtatt tagaggcagc acaaaatcaa aaatattagt
     4741 ttaaagagtg gttgtttcaa aacatattta aatctgtttt gaggcaacca ctaatttatt
     4801 ttattagatt ttgttgatat tttagttttc aaaaagtatt tgattaaacc agatataaaa
     4861 tgtcaaaaat aaaaattaac tgagttaaat aattttaata aaattggtag tatatgtgat
     4921 aaaattaaat attaaagagg atataggata ttagaatatc aattggtgaa ataatgtcat
     4981 atacgataaa ttaaggtgta agtttttggg tatacctatc ttaggagtga aaagatttga
     5041 gaataagtta tagaaattta aagcgatata gagaaattgg atatgtttta ataaagtatg
     5101 gttttagttt tattgtagaa agactaaata tagaaggaat tgcctataaa ataccactat
     5161 ttgacccacc agaagaaata aaaaatatga caactggtga gagaatgaaa agagttttag
     5221 aagaactagg acctacatat ataaaaatag gacagatatt gagtacaaga aaagacttgt
     5281 tagaccagga tataattgat gaaatatcaa aactcagaga tgatgtagag aaatttgatt
     5341 caaatatagc tattgatata tttaaagaag aagtgggttt aagtatagaa gaaatattct
     5401 tagaatttaa agaagaacca atagcagcag cttcaattgg tcaagtttat gaaggtgtac
     5461 ttaaaacggg agaagaagta atcgtaaaaa ttcaaagacc aaatatagag aaaataataa
     5521 aatctgattt agagattctt agaactattg caaatacttt aaaagattta aaaaaggatt
     5581 ttaatttaga tttagtacag atgattgaag aatttcaaac tcaattaatg agggaacttg
     5641 attatacatt tgaagccata aatgcaacta aattctctag gatatttaaa aatagtgatg
     5701 aagtatatat acctaaagta tactcagaat ataatactaa gaaaatttta gtaatggaga
     5761 aagttaatgg tacaaagtta agtgatgtag aaaaaataag gagactaggt tataacacaa
     5821 aaactattgt tgaaatagga gttaggtcat tttttacaca ggtattatct catggatttt
     5881 ttcatgcaga cccacatcca ggtaatatat ttgtagttgc taaaaataag atagcatata
     5941 tagattttgg aatgattgga attattgata ataaaacttt aaaccaatta aatgaaattg
     6001 cattagcagg tgtagaaaag aatgttgaca agataatata tctattgata gaaatggatg
     6061 cattaaatgg agaggctgat attaagggac ttagacagga tttactttat ctaatacatt
     6121 attactatga tatttctata gaaaaaataa atgtaacaga tatattaaat gaactattta
     6181 ggttttttag acagtataaa atagtcatgc ctgcacaatt tgtaactctt gcaaagacag
     6241 ttataacatt agaaggaaca agtagaactt taaacacaga tttttctttt ggttctatgg
     6301 gaaaagagtt tatgaaacat cattataaga gtaaatttaa ccctaaaaat gttgttttaa
     6361 gttcaagaca aaatgtagaa gaaatattac tggacataaa aactattcca aagcaattaa
     6421 aagcaatttt aaaaaatata gaaagaaata atataaaaat gcaaattgaa gatgttaaaa
     6481 tgacgaggtt agaaaattgt ataatagagc taacatctca aatatctttg agtttagtgt
     6541 tggcatctat tatagtaggt tcttctctta taattgcatc tccaaatata gaaaataata
     6601 tatggataaa atttactgca atagcgggtt tctttatatc ctttataata ggcctttgct
     6661 tagttataag aagtattagg tcaaaataca aaaaagatta gggggaatta aatttgaaaa
     6721 aatggatatt gaagtataaa ggtaagatag aggaaagtga atttagcaaa aaacttaata
     6781 tttccccgga aatctgccaa attttaaaaa atagagggat atgtactgaa aaagattcag
     6841 aaatatttat gaatccatct ttagattatt taagagaccc ttttttaatg aaagatatgc
     6901 aaaaagctgt tgatagaata aagttagcag ttgagaagaa cgaaaaaatt tggatttacg
     6961 gagattatga tgttgatggt gtatcatcta catccatctt gtgtttatat tttaagagta
     7021 taggttacaa tgttgattac tatattccta atagattaga agaagggtat ggtataaatg
     7081 aggatgctat taagcttata agctctaggg gatgtgactt gattataagt gttgactgtg
     7141 gaattacatc agtgtctgag gtcaatacag cgaatgattt gggcatagat gttataatta
     7201 cagaccatca tgaatgtcaa agtgaaattc catcagcctt tgcagtaata aatccaaaac
     7261 aagaagattg taattatcca tttgattcac tatgtggatg tggtgtagca tttaaaatga
     7321 ttcaagcctt gactccaaaa gaagaattta aaactagtat gtataattat ttggaaatag
     7381 taacactagc aactatttgt gatattgttc cacttataga tgaaaataga ataattgtaa
     7441 aaaatggttt aaaatcaatg aaagaaggca aaaatattgg attaagggag cttataaaag
     7501 tttgtggtgt agaaagtgat aagataggtt cttctcatat aggttttgca ataggtccta
     7561 ggataaatgc ttcaggtcga ttgggatatt cttacttagg agtagagcta tttactactc
     7621 agtcacaaga agaggcagta gaaatagcta gtatattgga agaaaaaaat aatgaaagac
     7681 agatgataga agctaagatg tatcatgaag ctgaagaaat gctaaagtct aatagcagat
     7741 acaatgatga caaagtgttg gttttagcca aagagggttg gcaacatgga ataataggaa
     7801 ttgtagcatc aaagttaact gaaaaatact acaaaccaac aattcttcta ggtatagaaa
     7861 atggggaggc tactggttca gcgaggtcaa taaaaggatt taatatattt gaagctctca
     7921 taaagtgcaa agacttgatg actaagtttg gtggtcatga acaagcagca gggttatcct
     7981 tggattcaga caatgtagaa attttagcaa atgaaataaa taaatttgca gattataatt
     8041 taactgaaga tgatatgata gagaatgtaa atgtcgagtt tgaattacaa gaaaatgtaa
     8101 taaatttaaa tcttgtagaa gaattacata agttagaacc ctttggatta aataatccta
     8161 atcctagatt tatagttaga aattatattt taaaagattt gaaagttata ggtaagaatc
     8221 aacaacatct gaaattaagt atagaaaaag agaaaagcta tgaatgtata ggatttaata
     8281 tgagtcattt aaaatcaatg tataaagtag gagacaaagt agatgtacta ttccaattag
     8341 atgaaaataa ctatatggga aataggaaag tacaattttt acttaaagat atacgattgg
     8401 ctcgacctaa aagtgcaagt aatgacaaac tttcattgaa attaatgtca aaaataatac
     8461 caaaagatac tcaaagcctg tataatatat ctgtgtctga ttttgaatta tttgatggaa
     8521 atacagatat aaatatattt gattattttg aaaaagatac actaataata tctaattcta
     8581 taaatggatt ttatagagca atgtcagata tatcacttat agatttggat tttaacataa
     8641 attacaatat tattgaagat gttagcaaaa atactgataa attagagctt atattttctc
     8701 ctaatattga taaaatagac ttaaaaagat ataataatat tattctttat gattatttat
     8761 acaataaagg cgaatattcg tatatatatg agaataaaag agaagaaagt gaaattataa
     8821 aatattataa taaaacagac ctattatatt taaagaatgt agttagtaat atagttccat
     8881 caagagatga atttataaca atttataaac aagcacttat aaaaaaagaa attgatttgg
     8941 atatggtaaa tataagggaa acttttaatg taattccatt aaagtttttt acgatattaa
     9001 atgtatttag agaattaaat ttacttgact ttaatttaaa ttatgaaaaa aattctgtac
     9061 tcataagaat attgccaaaa ccacagaaaa aattagactt aaatgagagt ttaatactca
     9121 ataatctaaa aaatttagaa aaacaataca atagcagtta ttaggaggcg tttcccatgg
     9181 atttaaaaaa ctttataagg aatattgatg atttcccaaa accaggcata gattttaaag
     9241 atgtgacaac tctatttaaa gatggagatg catttaaata tgcagtggat tctatagttg
     9301 aagaattgaa agataaagat gtagatttag taatagggcc agaagcaaga ggatttttga
     9361 tgggtacacc tgtagcatat gctttaggag taggatttgt tccaattaga aaacctggaa
     9421 agttaccagg tgaagttgag agttatgagt atggattaga gtatggtaca gatactttag
     9481 agatacataa ggatgcaatt aaaaagggtc aaaaagtagc aatagtagat gatttattgg
     9541 ctactggtgg gactatggaa gccgctgcca agcttgtaga aaaattaggt ggagaagtag
     9601 tttctatgca atttttgatt gagctaaaat tcttaaatgg gagagaaaag ttgtctaact
     9661 atgatgttaa ttctcttata aaatactagt acttttataa tagttggtat aacaccaact
     9721 attttcatat atattaaaat tttatacaaa ataggtggtt atatgcatga taaagaatta
     9781 caagaaataa ttgaaaaaat aaaagtttat gcacctaatg caaattttga aatggttgaa
     9841 aaagcctata atcttgcaaa aaaagcgcat gaaggacaat atagaaaatc tggggaacca
     9901 tactttattc atccattagc agttgcaaat attttaatag agatggaatt agatattgaa
     9961 actataacag caggtttact tcatgatgtt gtagaagata cagaatatac ctatgaagac
    10021 ataaaaaaag aatttggcaa agaagtagcg gatttggttg atggagtaac taaacttggt
    10081 caaataaaat atcgttcgaa agaagaaact caatctgaaa atttaagaaa aatgtttttg
    10141 gcaatggcga aggatataag ggttatactt ataaaacttg ctgatagatt gcacaacatg
    10201 agaacattaa aatacatgcc tccagaaaaa gcaaagtata aagcaaaaga gacacttgaa
    10261 atttatggtg gtatagctca tagattagga atttccaaga taaagtggga actagaagac
    10321 agagcattaa gatttatgga cccagaaggg tattatgatt tagttagtag agtatcaatg
    10381 aagagaagtc aaagagagga ttatattcaa ggaattgttg aacttcttaa agataagttt
    10441 aaagatttaa gtataccatg tgatgtatat ggtagaccaa agcattttta cagtatatat
    10501 agaaagatgc aaaaaaaaca taagactttt gaagagattt ttgatttaac agcagtaagg
    10561 atattggttg ataatgtaaa ggattgctat gcagtacttg gtatagttca tacattatgg
    10621 agacctattc caggaagatt taaagattat atagcaatgc caaaacccaa tatgtatcaa
    10681 tcattacaca ccactgttgt aggtcctgat ggagaacctt tagagataca aataagaact
    10741 catgagatgc ataatatagc tgaaaatggt atagcagccc attggaaata taaagaaggt
    10801 atctctagca aagagtcaaa ggttgaagaa aaacttcaat ggttgagaca gatgatggag
    10861 tgggaaaaag atttaaaaga cccacaagag tttatggatg cacttaaaga agatgtattt
    10921 aatagtcaag tttatgtatt tacgccaaaa ggagacgtaa ttgaattacc agcagaatca
    10981 acacctatag attttgccta tagagttcat acaaatgtag gtaataaatg tgtaggagct
    11041 aaaattgatg gaagaatagt tcctattgat tataagcttc aaaatggtaa tattgtcgag
    11101 gtacttacat catctaattc taatggacca agtagggatt ggcttaatat agtgaagacc
    11161 ccaaatgcaa aaagtagaat tagacaattt ttcaagaagg aaagaagaga agaaaatgtt
    11221 gaccgtggaa atacaatatt agaaagagaa tttaagaagc atgatatacc acaaaaagac
    11281 cctcttgttg aaaaatatat ggcattagtt gctaagaaat ttaatcagcc aagtgttgag
    11341 gacttaattg ccactgtagg ttatggcggt ataatgtcat ctcaagtggt atctaaggta
    11401 aaagacctat atttaaaaga ggttaaaaaa gctaaaaagg aacaacagat acaagaagaa
    11461 gatttaaata aacacaatat aagtgaaaca gaatacagta aaaagagaaa gaaaaacgca
    11521 tcacaaggaa ttatagtaaa agggcttgac aatatcttag taagatttgc taagtgttgt
    11581 aatcctcttc caggagatga aattgtagga tatattacta agggtagggg agtagccata
    11641 cacagagctg attgcccaaa tgcaaacaat aatggtgatt tcttccaaaa tagattggtg
    11701 gaggtttcat ggagttcagc aggaaaaggt aagtttgaag cggaaattca aataaaagct
    11761 attgacagaa gaggaattgt taatgaaata actcatattg ttgcaatgga taaacttagt
    11821 ttaaacggaa taaatgcaag aaaaggtaag gatagtattg taagtataaa cttacttgta
    11881 gaggttaatg acacagagga attaaaaatt ctaatgaaaa aattaaaagc aattcctggt
    11941 gtagaaagta tttatagaat gacaaattag agttaaaata atagggagga aactatgaga
    12001 gcagtagtgc aaagagtatc ttcatctaaa gttacagtag atgaaaacac tataggtcaa
    12061 ataaataagg ggttactagt attgttagga gtgactcatg atgataaatc aagtgatgta
    12121 gattatatga ttgataaaat attgaatctg agaatttttg aagatgaaaa tgacaagatg
    12181 aatttatctc taatggatat aggtggagag cttttagtag tatctcaatt tactttatat
    12241 ggagattgca gaaaaggaag aagacctgga tttagtaatg catctagacc agaacttgca
    12301 aataaccttt atgaagaatt tgtaaaaaaa gcaaaagata aaggtgtgac agttggtaca
    12361 ggtcaatttg cagctcatat gatggtagag ttaactaatg atggtccagt aacaatacta
    12421 ttagactcaa gtaaatcttt ttaaggggga aaactttaat gattataaaa aaatttgtag
    12481 atagttattt tggagtaaat acttatgtat taggtgatga aaagactaaa aaatgtgcag
    12541 ttatagaccc aggtggaagt ttagtagaga cattaagttt tgtaaaagaa aatgaattaa
    12601 atattgaata tattatcctt actcatgggc atggagacca tataggatat gttaaggata
    12661 ttaaagaaaa aactggtgct aaagtagttg cacatgttga tgaaaaagaa ttattaaatg
    12721 ataaaaataa aaatctaagt tatacaatga gatgtggagc gcaagagttt gatgcagata
    12781 tctatgtgag tgataaagac aagcttgaac ttggtgaatt aaaaatgact ttcttgcata
    12841 ctccaggaca tactcaagga tgtatgtgta taagagttga agatgaaatg ttcacaggag
    12901 atactttgtt tgctggaagt attggtagaa ctgatttata tagtggagac tttaaccaaa
    12961 tggaaaaatc acttaagaaa ttatgcaaat atgaagatgc tgttagagta tatcctggac
    13021 atggaccaac cagtactcta ggggttgaaa agaagacaaa tccttacatg taattttata
    13081 ttagtattta aaaaagtata aaatatttaa taactatatt agtgaggggg ttcttctact
    13141 gttaaataga aggagttaag ttgatgttgt atgttttttt aaaaggacat gattataagt
    13201 atgaagtagc agaacttata aaattgttta ccagtgaatt taagtttaaa gactctaatg
    13261 ctaacaataa tatagagtca aagcatttaa taaatagatt gatttatgag aatggtgtat
    13321 tattctcaag tactgaatac tatgaaaatg gaaatttaaa gtacgagtca atacagaata
    13381 tagaaaatat gaatcttgaa tttttagatg gatttgatag tagcaaaacg attgaagaat
    13441 ttcttaatag actagatagt gaaagtaaga ggaactttaa aaagctgtgt aaagaaaata
    13501 tcaaaaaaag catgtttgta gtgcttaaac aagtatttaa ttcttatgta ccatggggaa
    13561 ttttgactgg tattagacca gtcaaaattg tccacaattt aatggataaa aagctagatg
    13621 ataatagcat aagaaccatt ctaaaagata attattttat aatagataag aaaatagatt
    13681 tagctcttga aatagctaaa agagagcgat tatttatgta tcctatagat aaaaataaaa
    13741 tttctcttta tgtaagtatt ccattttgtc caactagatg tgtatattgt tcatttcctt
    13801 caaattcatt aaaacaattt ggacatttaa aaagagaata tgtttataaa ttgattgaag
    13861 aaattaaggg gtttgccaaa gttattaagg agactaagaa agaagttgaa actctatata
    13921 ttggtggtgg aactccaact acacttgatg aggaagaatt agatttgctt ataaattcat
    13981 tgttcaatga attagattta agtaaaatta aagaatttac tgtagaagca ggtagacctg
    14041 acactattac agaacaaaaa ctaagagtgt taaaaaaaca caatgttagt agaattagta
    14101 taaatcctca aactatgaat gatgatacct tggtaaagat tggaagagag catagagtat
    14161 ctgatttagt agattgcttt aatatggctc gtagaatggg atttgataat ataaatatgg
    14221 atataatttt aggtcttata gatgaagaat taaatatggt aaagaacaca ttggaggaga
    14281 taaaaaaact ttctcctgaa agccttacag ttcatacatt agccataaag agagcatcca
    14341 acttaaatat aaatatggat aggtataaag aacatctaac acaatatgaa gaaatggtaa
    14401 aaatgataga tttatcaatg aaatatgcaa aagatatggg attaaatcca tattatatgt
    14461 acagacaaaa acatatgttg ggaaatttag aaaatatagg ttatgctaaa gaaggatatg
    14521 agtgcattta taatatacag attatggaag aaaaacaaag taactatgca ttaggtgcag
    14581 gtgctatatc gaaatttgtt tatgtagatg aagatagaat tgaaagagta gaaaatgtaa
    14641 aaaatgttga acaatacatt gaaagagtag atgaaatgat aaaaaggaag aaagaagagg
    14701 tatataaaaa tgttgactaa agctcctaga ggaactaaag acataactcc aaaagaggct
    14761 tacaaatggc gttatgtaga aaataaattt agagaaatat gtgctttata tggatatgaa
    14821 gaaatggtaa cacctatatt tgaacataca gaacttttca agagaagtgt tggagatact
    14881 acagatatag ttcaaaaaga aatgtattct tttaaagata aaggtgatag agaaattacc
    14941 ttaaagccag aaggtacagc aggtgtagta agagcattta tagaaaataa attatatgca
    15001 gatactcaac caacaaaatt attttatgtg acaccttgtt ttagatatga aagaccacaa
    15061 gctggtagac aaagacaatt tcaccaattt ggaatagaag cacttggaag tgatactcct
    15121 tcaatggatg ctgaaataat cgcactagct gttcaatttt ttaatgaagt tggattaaat
    15181 gatttggtag tgagtataaa ttcagttggc tgtccagttt gtagaaaaga gtacaatgct
    15241 ttattaaaag agtatcttga ctcaaaagct gatatattgt gtgatacttg caatgagaga
    15301 agagaaaaaa atccaatgag ggttatagac tgtaaaaatc ctacatgtaa agaaaatatc
    15361 aaagatatac catttattgc agaccatttg tgtgatgatt gtaaatctca ctttgataaa
    15421 cttcaagaat acttaaagga aatgaatata aactttgtaa ttgataaaac aatagttaga
    15481 ggcttagatt actatagaaa aactgctttt gaaattatat ctaatgatat aggagctcaa
    15541 agtactgtat gtggtggagg aagatatgat ggtcttgttg aacaattagg aggaccaaaa
    15601 ggtataagtg gaattgggtt tggactagga attgaaagac ttttacttac acttgaaggt
    15661 aatggaattg aaatagaaaa ccctcaatct acggatatat ttatagtaac tataggtgaa
    15721 gaagcaaata caagaagctt taaattatta aaagacttga gacaaaatca tataagtgca
    15781 gataaagacc atatagagag aagtgttaaa gcacaattta aatattcaga taaaataaat
    15841 tcaaaattta caatagtgat tggagatgat gagcttaaaa atgacactgc gacattaaaa
    15901 aatatgaaaa catcagagca aactactgta aaattaagta cattagttga agaattaaag
    15961 caaaagctgt aaaataagaa agtatgatgt tttgtgtatt agataaatat atagcattaa
    16021 aattaaataa aatggaggta gtatcttgga aactttaaag ggtttaaaga gaactcacta
    16081 ctgtggagag ttgagagaaa aaaacataaa tgaagaagtc gtacttatgg gatgggtaca
    16141 gaaaaaaaga aatttaggtg gtcttgtatt tgttgattta agagatacaa gtggattatg
    16201 tcaaatagtt tttgatacag atgtagataa agaagctttt gaaaaagctg aaaaattagg
    16261 ggctgaattt gtaatagctg taaaaggaaa agtatgcgaa agacaatcta aaaaccctaa
    16321 tatgccaact ggtgatatag aaatatttgc aactgaactt agacttttaa acaagtcaga
    16381 aactccgcct atttatataa aagatgatga tgatgtttct gaagcactaa gattaaaata
    16441 tagatactta gatttaagaa aaccatctat gcaaagaaat ttaaaattaa gacataaagt
    16501 aatgaatatt acaagaaatt acttatcaaa caatagattt tgcgaaatag aaacaccatt
    16561 tttaatagct ccaacaccag aaggagcaag agattatcta gtaccaagta gagttaatcc
    16621 aggaaaattt tacgcattac cacaatcacc acaattatat aaacaattat tgatggtaag
    16681 tggtatggat aggtatttcc aaatagttaa atgctttaga gatgaagatt taagagcaga
    16741 tagacaacca gagtttacac aaatagactg tgaaatgtct tttgtcgagc aagaagatgt
    16801 tatgagtatg attgaaggtt tgcttgaagc gatatttaaa gaagttttag atgtagaatt
    16861 ggcacttcca cttcctaaga tgacttatgc agaagctatg tctaaatatg gttcagataa
    16921 accagatact agatttggat atgagttaac agatatatct gatgttgttt gtaactgtgg
    16981 atttaaagtg tttgctgatg ctacacaacc aggaaaaagt gttagaggta taaacgtaaa
    17041 aggcaaagct gacgacttta ctagaaaaca aatatcatca ctagaagaac atgctaagac
    17101 atatagagca aaaggtcttg cttggatgaa agtaggacaa gaaggagtaa cttctccaat
    17161 agcaaaattc tttaatgaag aagaaatgaa cgctatactt acaaaaatga atgctgaagt
    17221 aggagattta cttttatttg tagctgataa aaactcaata gtatttgatg ctttaggaca
    17281 agttagactt gaagtagcaa ataggcttaa tctattggat aaaaatgtat ataacttact
    17341 atgggtaact gagttcccag tttttgaaga agatgaagaa acaggcacat tttcagcaat
    17401 gcatcaccca tttacatcac ctatggatga agatttagac aagttagaag aaggtgataa
    17461 gtcatcttta agagcgaaag cttatgatat tgtattgaat ggttatgaaa taggtggagg
    17521 aagtgttaga atatcaaatt ctgatattca atcaagaatg tttaaagcac ttggtttcac
    17581 agaggaaaga gctaatgaaa agtttggtta tttattagaa gcatttaaat atggtactcc
    17641 tcctcatgct ggtctagctt ttggtcttga tagacttgtt atgttacttg caggaactga
    17701 taatataaga gaagttattg cattccctaa aaatcaaaat gctgtttgcc ctatgacaaa
    17761 tgctcctaca ttggcggaag atgagcaatt agaagaatta agtataaaag ttgatataaa
    17821 agataatgaa taatatgtag ttgcaagaaa ctaaaatgta aaaagtctgg atataaagta
    17881 tatttatatt cagacttttt ttatta
//
LOCUS       DAEPLF010000062        17897 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEPLF010000062 DAEPLF010000000
VERSION     DAEPLF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.062"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            235..3783
                     /locus_tag="KR314_001790"
     CDS             235..3783
                     /locus_tag="KR314_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0328176.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINNEMVIALMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTISCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKIIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDNFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADMALY
                     SAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESASNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMRNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEVTSIEAEENT"
     gene            3968..5902
                     /locus_tag="KR314_001791"
     CDS             3968..5902
                     /locus_tag="KR314_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBH0328177.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLENAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            6008..7003
                     /locus_tag="KR314_001792"
     CDS             6008..7003
                     /locus_tag="KR314_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0328178.1"
                     /translation="MKKYISIILLVVVTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSNIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6975..7760
                     /locus_tag="KR314_001793"
     CDS             6975..7760
                     /locus_tag="KR314_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBH0328179.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7748..8446
                     /locus_tag="KR314_001794"
     CDS             7748..8446
                     /locus_tag="KR314_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328180.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8569..9042
                     /gene="ybaK"
                     /locus_tag="KR314_001795"
     CDS             8569..9042
                     /gene="ybaK"
                     /locus_tag="KR314_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBH0328181.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9239..10078
                     /locus_tag="KR314_001796"
     CDS             9239..10078
                     /locus_tag="KR314_001796"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBH0328182.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10091..10639
                     /locus_tag="KR314_001797"
     CDS             10091..10639
                     /locus_tag="KR314_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBH0328183.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10705..11886
                     /locus_tag="KR314_001798"
     CDS             10705..11886
                     /locus_tag="KR314_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBH0328184.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYIYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12059..12322
                     /locus_tag="KR314_001799"
     CDS             12059..12322
                     /locus_tag="KR314_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0328185.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            12848..13531
                     /locus_tag="KR314_001800"
     CDS             12848..13531
                     /locus_tag="KR314_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0328186.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGEFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            13542..14783
                     /locus_tag="KR314_001801"
     CDS             13542..14783
                     /locus_tag="KR314_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0328187.1"
                     /translation="MKIFSNKDIKIFFLILISILVMFIIVGQIVVISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDLFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIVLNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISINGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSTFTISFLKLTNM"
     gene            14855..15538
                     /locus_tag="KR314_001802"
     CDS             14855..15538
                     /locus_tag="KR314_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328188.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            15535..17865
                     /locus_tag="KR314_001803"
     CDS             15535..17865
                     /locus_tag="KR314_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0328189.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDSGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANTGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KPEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNRNAEKELEVGG
                     ILSSIPFEAKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTPGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAMSGCLLGSILGLMLHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPVKKICELDIIDSINAQ"
BASE COUNT         7165 a         1818 c         3233 g         5681 t
ORIGIN      
        1 acattattac tttttgataa atcaaaaata aagttatagt ttttaatatt aattaagaat
       61 gatgaaatat gaataaagat tttacaaata aatcttaagc tatcttattt aattctaatg
      121 caggaagaag gtaaatgtat attgaaaaaa tcatagaaat agaagtaaac aacttaaaca
      181 aatacaaagg tttgcaaaag aagtaatata attatataca atgaggatgt gagtatgatg
      241 aacagactaa agccaagaat tttaggtatt agtatcttgt ttattattat aattatttta
      301 acatgtggag ctgttttcaa gtttaaattt aaattagata ataaattaaa agaaaccaca
      361 catacatatt tgtctgagtt tacagaacac aatataatta atctcaagac aagacttagt
      421 ggtcaatttg acatgcttga atctattgct aacttcatag gaaacttgaa taatataaat
      481 aatgaaatgg tgattgcttt aatggattct gatgtaaagc gaagttcttt aattagaatg
      541 actatctcta cattagatgg ggaatgttat tctaatgatg gaatcaccca taatgttcaa
      601 gatagagatt attttaaaaa agctaaaaat ggagaaaaga atatttcaga gcctttaaaa
      661 tctattgtta ataataaaga aataatagtt ttatctaccc cagtttataa aagtgggaaa
      721 atagttggtg ttttaagtgg aatttatgat tcaaaaaagt taaatgagtt attaggatta
      781 tctgcatttg atgaaaaagc agatgttttt gttgcaaaga aaaatggaga tatagttgct
      841 caaaaaacaa ataaaaatag aaagacgata tcatgtatat ttcagctttt tgacaaagat
      901 aattgtgatg aagatgattg tctaaaaact ataaaagaaa atatgaaaaa tgataagtct
      961 ggatatttta attataactt agataatgaa aaatttatgt taaactatca gccacttgga
     1021 gttaatgatt ggtatgtttt ttctgttgtg ccaaataaaa tagtgtctat tcagtcagag
     1081 aatatatctt atgatgtata tatttttgca ttagaaatac tactagttat attgattttt
     1141 ataatatata tagcatgtat tataaataat aacaccaaaa ttattataaa tgaaaaagaa
     1201 aaatttaaag tactcactga taatatatta ggaggggtta aaacctgttt aaaagatgaa
     1261 tatatgacta tgacatatgt aagtcagggg tttttagagc ttacaggtta ctcttgtgaa
     1321 gatttaaata aaatatttaa aaatagattt aaagacatga tttataaaga tgatttagac
     1381 aagtttaatt gtattattct aaatcaatta gaaaaacaag ataaaattga agttgaatat
     1441 agaattgtaa gaaaagatgg aagtataatt tggattttgg agagaagtaa gtctataaat
     1501 gataataaag gaagtgagtg tattcaggga gttttaactg atataacaga acttaagaaa
     1561 atacaagaag agttagattc aaaaagattt gaaatacagg ctatcaatga tagtattgtt
     1621 ggtggaatag tgataactga gataaatgat gattttgata gtatatattt aaatgaaggt
     1681 tatttaaata tgattggata tacacgcaaa caactggaag atgagctaaa taataaatta
     1741 atcaaactta tttttaaaga tgatagaaca gaattaatta atagtataaa caaacaactc
     1801 aaattaagtg atacatttgc atctgaacaa agagttgaaa aaagggatgg tactattata
     1861 tggattttat taaaaggaag aaagataatt tatgataatg gaaaatctgt actttgttgt
     1921 gttattgtag atatcacttc tgctaaatta ttagagcaag aacttaaaaa gagtgaagaa
     1981 cgtcatgaaa tcattctaaa gcagacgcaa aatatcataa tagaatggga tatagaaaaa
     2041 gataaaatga atatatcacc taattggtat aataagttta aatatgaccc tataactgaa
     2101 aacgcaacaa aaaatataat taaatcagta gatatatatc atgatgatat ggataagttt
     2161 atttcattaa ttaaacttac ttcacaaggt agtgagtatg aagagtgtga aattagactt
     2221 agaactagag atactattta cgcttggttt agtataaagg caacaacttt atttgataat
     2281 aatggtaaac cttataaagt agtaggaagt ttgactgata ttgatgaatc aaaacgagaa
     2341 gctgaaaagt tgaaagaaag ggcagaaaaa gaccctctta cagatttgta taataaaaaa
     2401 gcatcacaat cacttataga agagtacata acattggata aaaaaagaaa aggtgcttta
     2461 atgattattg atattgataa ctttaaagga ataaatgata atttaggaca tttatatggg
     2521 gatgctgtat taagtgaaat tgcaaatgac ctaataaatc tatttagagc aaccgatata
     2581 gtaggcagaa ttggtggaga tgagtttatt gtatttataa aagatatttc tgaaacaaga
     2641 gatattataa ataaagcaga agaattggta aaaacatttg aaaggtcttt tttaggtaaa
     2701 ggacgaaatt ataaaatatc attaagtgta ggaattgcta agtttccaca agatggtatg
     2761 gactatatga cactatttag aaatgcggat atggctcttt actcagccaa agggaagggt
     2821 aagaattgtt atacattgta tgataaaaaa attgataaag tccaatatat aactaataca
     2881 agaaatgcag gagatagtga taatgtagag atgtctttta aagaacatat cacaaattat
     2941 atatttgata tactatatga atcaagtgat atagaagtgg cagtaaattt aattctaagt
     3001 attgtaggta aacactttaa tgtgagtcgc atgtatatat ttgaaaattc agatgataat
     3061 cgagtatgta aaaatacatt tgaatggtgt aacgaaggtg taacctcgga aattaataat
     3121 cttaaaaata tttgttacaa ggatttaggc aactatcttt taaattttaa tgcagatggg
     3181 ttattttatt gtaatgatat tgaaaattta ggtagtgatg tgaaaaaagt acttaagcca
     3241 caaggcatac gttcaatatt acaatgtaaa attttagagg gcaatgaatt tagagggttc
     3301 ataggatttg atgaatgttc atataatcgt atgtggacta atgatgaagt gaacactttg
     3361 acatttatat ctaaaacgct cagtatgttt ttattaaaaa tgcgtattca gagtaagcta
     3421 gaagagtctt taacaattca aaaatctgtg atggataata tggatatatg ggcatatgtt
     3481 gttgacagga atacctatga attattattt atcaataaaa aaatgcttga gtcagcatca
     3541 aacactcatg taggtgattt atgttataaa gcgatatgga ataatcaaga taaaccttgt
     3601 aaaaaatgcc ctatgatata tttaaaagat ggtgatgata cttataaatc atttatgcgc
     3661 aataaaaagt ttgaggtaca ggcaaatata actacaacta ctttgaaatg gtttgatggg
     3721 aaagatgcat gtttaatatt tggaagtgaa gtaactagta ttgaagctga agaaaatact
     3781 taaaatactg tgttatatat atacttaaaa tcgcaatttg aatttattag acttaaaatg
     3841 tctatataaa tcatttaagt tgtggtttta agtatttttt atgatataat taagcgttaa
     3901 gaacaaaatt aaaagatact tttgtataaa aaaatatttg ttaaatatat attgagcagg
     3961 tgataatatg gaaaataagt ttttaccaat aagcaaacaa gatatgatag atagaggatg
     4021 ggaagaatta gattttgttc tagttacagg agatgcttat gtggaccatc atagttttgg
     4081 aacagcaata atatctagag ttttagaaaa tgctgggtat aaagttggaa taatagctca
     4141 accagactgg agaactacag atgattttat gaagctagga aaaccgagac ttgcatttct
     4201 agttaatgca ggaaatatgg attcaatggt caatcattat tctgtaagta aaaagcatag
     4261 agaaaaggat atgtattctc caggtgggaa aatgggttat agaccagata gagctactat
     4321 agtatattgc aataaaataa gagaagcata cagtgatgtt gcaatagtaa taggtggaat
     4381 tgaagcgagt ttaagaagat ttgcacacta tgattattgg agtgataagg ttagaaagtc
     4441 tatgttaata gatagtggag cagatttatt agtttatggt atgagtgaaa agcagattgt
     4501 agaagtagcc aatgcactta atgatggata tgacccaaaa tatattagac atattgatgg
     4561 gacatgctat atttcagata cattggaaga aatatatgat aaatatatat taattccatc
     4621 atataaagaa atttgtgaag ataaaatgaa gtatgtagaa gcttttaaaa ttcaatatga
     4681 tgagcaagac ccatttagag gtaatataat tgttcaacct catggaagta agtatctggt
     4741 tcaaaataag ccagaaaaac cacttagtag agaagaacta gatgaagtat atggattacc
     4801 atatcagaag acataccatc ctgtgtatga aaaatttgga ggaataccag ctatagaaga
     4861 agtaaaattt agtatagtaa gttcaagagg ctgttttggt agttgttcat tttgtgcaat
     4921 aacatttcat caaggtagag cagtgcaaag tagaagtgaa aaatcaatta ttgatgaagc
     4981 aataggaata acaaatttag atgattttaa agggtatata catgatgttg gaggtccaac
     5041 agctaatttt agaagaccag catgtaaaaa acaaattact aaaggtgctt gtaaaaatag
     5101 acaatgctta tctccatcac catgtaaaaa tttagatgca gaccacagtg aatacttaca
     5161 tttacttaga gctgtaagaa aattacctaa aataaaaaag gtgtttgtgc gttctggaat
     5221 tagatacgat tatgtaatgg cagataagaa caataaattt cttagagagt tgatagagca
     5281 tcatgtaagt ggtcagttaa aggtagcccc agaacatata tcagaagaag ttttaaagta
     5341 tatgcaaaaa ccagcaggaa aaacttatga taaatttaga cagaagtttt ttgctataaa
     5401 tgaagaatta ggtaaaaagc aatatttgat accctattta atgtctagtc accctggttc
     5461 taccttaaat agcgctatag agttagcaga gtatttaaga gatactcact accaaccaga
     5521 gcaggttcag gatttttatc caactccagg gactctatct acaaccatgt tttatacagg
     5581 tattgacccg cttacaatga agccagttta tgttcctaaa tcaaaacgtg acaaggcaat
     5641 gcaaagggct ttattacaat atagagcacc tagaaattat gatttggtat actctgcgtt
     5701 agttgaagca ggaagagaag acttaattgg ttttggacat agatgtctta taaagccaaa
     5761 aaatgaaaag ccttatttta atagaaataa ttctaaaaaa aatgtatcta aaggaacaaa
     5821 taaaaacaaa aaaactaata caaataacag aagtaataaa aatcaacaaa aaagttcaac
     5881 taaaaaaaag aaaaaacatt aaagtataaa taaaaatact taaaaaagaa ggattattcg
     5941 ataaattaac agaaattagt ataaataaat ataattattt taatgacgtt acctttggga
     6001 ggaagagatg aaaaagtata tttctataat attattagtg gtggtgacta tggttgtttt
     6061 ggtaggatgt tcaccaggaa aagataaccc taaaaataag gagctatcag ttgtcaaaca
     6121 agtaaaagta gctgttccag atggactacc agcagtggca atagctaaac ttgcaaatga
     6181 aaatccagag attaaagaag gatatgaaac agtgtatagc attgaaaaga cacctgaggc
     6241 gatttccact agagtgatga aaaaagatgc agatatagct atagttcctt caaacatggc
     6301 tgcaatagcg tataataaaa cttcatcata taatattgtt ggaactgtag gaatgggttc
     6361 actttatttg gtgtcaacag aaaatataaa agattattct gatttagttg gtaaagaagt
     6421 tggatgtact ggaaaaggac ttactccaga cataactata aaaagtcttt tgttacaaaa
     6481 ggatataaat tattcaaata taaaatttaa ttatgtaaat tcagcaagtg aactggtacc
     6541 attattggct actggtaaag ttaagacagg tattgttcca gaacctgcac ttagtgcttt
     6601 gatgtctaaa aatccagaca taaaaattat aaaaagttta aatgactcat ggaaggaagt
     6661 aagtggttca aaagacggtt atcctcaatc tactttaatt gtaaagtctg aatttttaag
     6721 agataataaa gattttgttg attcttttgt aggtcaatta tcaaatagta tagattgggc
     6781 aaacaaaaat cctgaagaat taggtgcata ttctgaaaaa ataaaaatat ctactgaatc
     6841 aaagataata ggaaaatctc ttgaaagagc taatttaaaa tatattccag ttaaggacat
     6901 gattaaagat tataaaaatt actatgagaa attagctaat tttgatgata agacattagg
     6961 aggcaaagta ccagatgaag caatatactt tgttgagaaa taaaaaaaat aaagaacaat
     7021 ttatagataa aatggaggta acactttctt gtgttatcct cttattttta tggcaaatta
     7081 ttgctttaaa aattgataat gatatttttt taccaacagt aggtcaagta tttaatagta
     7141 taaaagagat tatgctaaat tctagtttct atatagatat actttactct atggggagat
     7201 gtttttttag ttttttactg gctatgattt ttgccattat actttcaata gtatcttatt
     7261 taaatagatt ttttagaaac ttattaaagc caatcaatgc tttgacacgt tcaataccaa
     7321 ctatgatatt ggtagtactt gcacttattt ggtttgaaaa ggatagtacc ccttttatag
     7381 ttggatttac tatagttttt cctatattgt atgataacgt tttgggcgct atattgaata
     7441 tagacaagaa cttattagaa atggctaatg tgtataaaat aaggtttata gataaggtgt
     7501 taaaaattta tttaccagcc ataaaatttc aaattatatc tatactagta tcaacattct
     7561 ctttaagttt gaaagttgct atagcaggag aggtatatag tcaacctaca tatggtattg
     7621 gaactatgat acagactgaa aaaattaact ttaatacatc tggaatattt gcatggataa
     7681 ttattgttct tttaatatct gctattttac aaatagccca aaagttttta gcaaggagag
     7741 cttttttatg gaaaagataa agttgagtat tgaaaatata aataaaagat atgatagtcg
     7801 tattatattt agagatttta atattgattt ttatgtaaat gaggtaaatt gtatccttgg
     7861 aaaatctggt tgtggaaaaa ctacactctt gaacataata agtgggatta ttaaaaatga
     7921 caccaacaat ctaaatatca aagaaaattt aaatagagta ggaaataagc ttgaagctag
     7981 ttacatattt caagatgata gacttataga ctggttaaca gtagaagaaa atataaagat
     8041 agttgtaaat aaatactata ataaaactca attaaataaa atttgtgatg aatacttgga
     8101 attggttgga attagtgatt ataaaaaatt ctatcctcag atgcttagtg gaggtattag
     8161 acaaagagta aatattgcta gagcttttat atatccatca aaaaacatta taatggatga
     8221 accttttaaa tctattgatg caaagaatac acagcttata atggataatt tcagaaatat
     8281 attaagaaaa gaaaagagaa ctgtattatt tgtaactcat aatattgagg aagcattgtt
     8341 tttagcagat aggatattta ttttaggaga ctctccaatt agaataaaaa aaatattaaa
     8401 aaattctaag gaattggaaa aaaatgaagt tttgaagttg atatagagtt ataataatac
     8461 tgtcgtttta aaatatattt ggttaaaatc tataagacta tattaaaata gtacagaata
     8521 ttcaaaactg tgtataatgt tcgtatttta taaaaaggaa gtgaatgtat ggttaagact
     8581 aatgctatga gaatattaga ttcaaataag attgattata aagtaatgtc ttatgaagtg
     8641 aaaagtgaac atgtggatgg tgttgaggtg gcacatgata taggaagaga tgtaaatgaa
     8701 gtgtataaga cattagtaac tcaaggagtt agtaagaata tatatgtata tgtaatacca
     8761 gtacatgaaa acttagactt aaaaaaggct gcaaaagtag ctaaagagaa aagtgtagag
     8821 atgattcatg taaaagatat aaataagctt acaggttata ttagaggcgg atgttctcct
     8881 ataggtatga aaaagcttta taaaactttt gtaaatgaaa gtgcaaagaa tttagataca
     8941 attattgtga gtgcaggaaa aataggatat caaatagaac tatctccatt tgatttacaa
     9001 agacttataa aagtagaatt tgtagatgta ataaaaaaat aagaattaca tataaaatat
     9061 agagcttata ttcttaataa taggtgttgt aatattaata aaatgaataa taattttaaa
     9121 ataaatattc agttacttat gtgtaattgg tataaaaata ttgagaaatt ttaaaaacaa
     9181 tggtaaagtg taagtataat cacgaatatt ttttataaag taatggaggg atgttcgctt
     9241 gagaaaaata aaatctgaac aaatcgtcga gcaggtaaaa aaactttgta tagaagcgag
     9301 cctatattta ggagaagatg ttttaagttg tataaaagaa aaagcgaaaa gcgaaaagag
     9361 tgaagttgga aaaaatatat tgaatatatt agttgaaaat gctgaaatag ctaaagaaaa
     9421 aaatatacca atatgccaag atactggtat ggctgtattt tttgttgaaa taggacaaga
     9481 agttttaatt gaaggagaca cacttactga tgctataaat gagggagtaa gacaagggta
     9541 tgaagaaggc tacttaagaa aatctgttgt aagtccaatt aacagagtaa atacaaaaga
     9601 taatacacct gctgttattc attatgatat ggtaaaaggt gataaaatta aaatagaatt
     9661 tgcagctaag ggatttggaa gcgaaaatat gagtaagatg aaaatgttaa agccatcaga
     9721 tggattggaa ggaattaaaa aattcataat agatacagtt tcagaagctg gacctaaccc
     9781 atgccctcct atggtaattg gagttggtat aggtgggact gtggacaaat gtgctcaaat
     9841 agctaaaaag gcacttttta gagaactagg tgaatttaat aaggacgaaa atatagcaaa
     9901 acttgaaagt gaactattaa cagctattaa taaacttggt ataggacctc aaggcttagg
     9961 tggtactaca acagctttgg gattaaacat agagactttt cctacacaca tagcaggtct
    10021 acctgttgtt gtaaatataa actgccatgc atcaagacat aagaaggtag taatttagga
    10081 ggaaaataaa atgataaaaa taactacacc tgtaaatgaa attgatatag caaaattaaa
    10141 ttgtggagat acaattagtt tgagtggtat actttataca gcaagagatg ctgctcataa
    10201 aagattgata gattgtataa ataaaggtga agaattacca tttgacgtat atggtcaagg
    10261 aatatattat gtaggaccta ctcctacaaa gccaggagaa gttataggtg ctgcaggtcc
    10321 aacgacgagt tatagaatgg atgatttgac tatacctctt ttagaaagag gtcttagatt
    10381 gatgataggc aagggaaaaa gaagtgatga agttatagaa ggtatgcaaa aatacggagc
    10441 agtttatctt gctgcaatag gaggagctgg agcgtatatt tcaaattcaa taaaatcatg
    10501 tgaaattatt gcatatgaag atttaggagc tgaagctata agaaagattg tagtagaaga
    10561 ccttaaactt acagtagcaa tagatagcta tggaaataat atatatgagc aaggtcgagc
    10621 aatttatgaa tgtaaataat gtttagaaat aaatatgaat atcaaaatta aaaatagttg
    10681 tgtatatttt aaggaggaaa attgatgtct aaggattatg caaaattggc attagaaatg
    10741 catgaaatca acaaagggaa agtaagtgta gaaagcaagg tagaagttaa aactaaggat
    10801 gatttatcaa cagcatatac accaggggtt gctgaacctt gtttaaaaat acatgaaaat
    10861 caagatgatg tttatatata cacttctaaa gggaatctag tagcagtagt tagtgatgga
    10921 agtgctgtac ttggattagg taacattggt gctgaagcat ctatacctgt tatggaaggt
    10981 aaagctatat tatttaaaca atttgcagat gtagacgctt ttccaatatg tttaaagact
    11041 aatgatgtag atgagatagt taaatctgta gagttgatgg agcctgtatt tggaggtata
    11101 aatttggaag acatatcttc tccaagatgt tttgagatag aagagagatt aaaaaaatct
    11161 ttaagtatac ctgtttttca tgatgaccaa catgggacag caataatagt tgcagcagcc
    11221 ataataaact caataaagtt aattgaaaat aaaaaaatag aagatttaga aatagttata
    11281 aacggagcag gagctgctgg tatagccata gcaaagatac tacttaatat gaatgttaaa
    11341 aatataatct tatgtgatag aagtggagca ttggaagcta gtatagaaaa tctaaactat
    11401 gttcaaaaag aaatgttaaa agtaacaaat ataagaaatg aaaaaggacc tttaaaagat
    11461 attataaaag gaaaagatgt atttattggt gtttcaggtc caggagctgt tacaaaggaa
    11521 atggttgaaa gtatgtctga gaagccaata atattggcaa tggcaaatcc tactccagag
    11581 ataatgccag aagaagcaaa attaggtgga gcatttataa tgggaacagg tcgttcagac
    11641 ttccctaatc aagtcaataa tgtactggct tttcctggaa tatttagagg agcattagat
    11701 gttaaggcta aagagataaa tgaagaaatg aagatagctg ctgcatatgc aattgctaat
    11761 acaataagtg atgaagaaat atgcccagag tatatattac cagatgtatt taataagaat
    11821 gtagtaaaaa atgttgctaa ggcagttaaa gaagcagcga taaaaactaa agtaaataga
    11881 atataaattt ttataactta ttaaaaacta gaaaagtata aaagactagt gaaattatgt
    11941 acagtgggat gattttatta gtctttttat tttaaatatg acacaataat gtcatattaa
    12001 gcttgatata attttaatat ggttaggagc caaagaaaaa tataaggagt gatttaaaat
    12061 ggaagaaact aaattttgcc aatgctgtgc aatgccaatg ggacaaactg atgaattata
    12121 tgggacaaat aaagatggaa gtaaaagtac tgattattgt agttattgtt ttaaagatgg
    12181 tgagtttaca gctgatataa gtatggaaga gatgatagaa gtttgtatcc ctcatatgct
    12241 tcaaagtaat aaggatatga ctgaagatga agcaagaaca atgatgaaca atttctttcc
    12301 aacattaaaa cgctggaaat aattttatgc aaaattagtt atatgcaaaa catgaaatat
    12361 ggtgttgaga ataaggggtg gtgtgcttta tagcatacca cctgttttgt tacttgctag
    12421 ttcagttatt cttgtatgct taaatatgtt ctcatctctc tttctatttt aaattgtaaa
    12481 aatctaaaat atgttattta tattctactt ctttatccat gtctttttct acctcaagtc
    12541 aaatacaact acaaaatcat cattttcaat tcactgttta atagaattaa cagataaaga
    12601 gttataaaat tttctatttt ttatataaaa taatacaggt caagctgact tctagctact
    12661 tgggatagtg tatatattta tgatagttat aggagtaata ccaatcatta gactgttgat
    12721 ttgtgtagtc ttttgtaaaa acatttgttt taatatattg agtttttgaa aaagtaaaat
    12781 tataattata ataaattaag tgtaataatg tattagaagt aaaaataatt aaaatagagg
    12841 tgattaaatg agcagtatat tgttagttga agatgattta agcttgatat atggattaga
    12901 gttctctata caaaaaagtg gatttagtat agacatagca aggacagtaa aagaagctat
    12961 gcaaatgtat gaggaaaaaa attatgactt attgctttta gatgtatccc tacctgatgg
    13021 taatggattt cagatttgcg aaaaagtacg agaggtttct aatataccca ttatattttt
    13081 gactgcttca gatgaagagg taaatgtagt gatgggtctt gatatgggtg gcgacgatta
    13141 tataacaaaa ccttttaaat taaatgaatt aatttcaaga ataaaagcat tacttagacg
    13201 ttataatgtt gcaagtaatg tgaatgaatt aagttcaaat aatattacta ttaaattact
    13261 tgaaaatcgt gtttttaaag gtgaatttga ggtggaactt actacagcag aatataaatt
    13321 attgtgctta tttatgaaaa ataaaaatat tgtacttact agaaaaaaca tattggacaa
    13381 gttatgggat ggaaatggta gctttataga tgacaataca ttatcagtat atgtaagacg
    13441 attgagaaat aaaattgaag ataatcctga aaatcctaag tttctattga ccgttagaag
    13501 aatgggttat aagtggaatg taattaagtg agggtattaa tatgaagata ttttctaata
    13561 aagatataaa aatatttttt ttaattctta tatctatttt agtaatgttt attatagtag
    13621 ggcaaatagt ggttataagt ataactaaca attataaaaa aacgttgata gaacatgatt
    13681 ataatatagc tggatactta tatagtatag gtgttgataa atctaaaata ccatcagcat
    13741 ttgttgcaca tgaagataaa tatattttaa aaggaaaaga aatactagat acagctggat
    13801 atggatttga tatagatgtt agctttacac caagtgtaaa attgtttcaa agtaaatata
    13861 taaaaactat gtctatctat ttattagtat tttttactat gattattttc atactatata
    13921 tgtatttttt taggcaacaa agtaagatag aagaggctag ttataaaatt gatttattta
    13981 tgaatggaaa tataaatgaa agactaaata catatgaaga aggtagctta tcaaaattat
    14041 ttagttcaat agatgttatg tcgacatctt taaatacaca tatattaaaa gaaaaacaga
    14101 gcagagaatt tcttaaaaat acaatttcgg atatatcgca tcaattaaaa actccactta
    14161 cagctttaat aatgtacaat cagatactac aagaagaaag tcataatagt gatgtcgttg
    14221 aagcttttgt acaaaaaagt caaagtgaac tagaacgcat tgaaacattg attaagaatt
    14281 tactaaagat tacaaagata gattcaaaaa caatagttct caataaaaat aatgttaatc
    14341 ttaaaaattt aattgtcaaa attttgtata gctttgaaac tagggcagaa aaagaaggaa
    14401 aggttatttc tataaatggt tcagaaaata cattgttgtt ctgcgattat gaatggatgt
    14461 cagaggcttt aagtaatctt ataaagaatg cattagataa tacaaaagag aatgataaaa
    14521 taacgataga gtggtttgag acgactataa ccaccattgt ttcagttaaa gacactggaa
    14581 atggaatcca tgttgaggat attcatcata tatttaaaag gttttataga agtaagtttt
    14641 caaaagataa tcaaggttta ggactgggat taccactagt taaatctatt gtggaacaac
    14701 ataatggtac tatttctgta gaaagtaaac tatcacaagg aagtacattc accataagtt
    14761 ttttaaagct tactaatatg taagataaaa tacatcttgc agtaaggtat atagattatt
    14821 attatattaa tcaattagaa aggtgagaga gaaaatggat attcttgtgg ttaataattt
    14881 atgtaaaaca tatggaaatg gatatacaaa agtagaggca ttgaaagatg tatcctttac
    14941 agtttcaaaa ggagagtttg tgactgtagt tggagaatct ggttcaggaa agagtacact
    15001 attaaatctc ttaggaggtc ttgatactcc aacatcaggg agaatattta ttgatggtaa
    15061 agaaatatgt tcaatgaaag aggaaaaaca gactattttt agacgtcgta acatagggtt
    15121 tatatttcag gcatataacc ttataccaga actaactgtg gagcaaaata taatattccc
    15181 attgttatta gattataaaa aaccagatac tatttactta gatgaagtat tgactatttt
    15241 aggtcttaaa gataggaaaa atcatcttcc aaatcaatta tctggtggtc aacaacagag
    15301 agttgcaatt ggaagggctt tgattacaaa gccaagtatt atactggcag atgagccaac
    15361 gggaaatctt gacagtaaaa atagtaatga tgtaatttca cttttaaaag tgactgcaga
    15421 aaagtataat caaaccatta ttatgataac tcataatacg aatatagctt ctacagcaga
    15481 tagaatacta caggtttctg atggtgtgct tacagacctg ggggtgaata gagaatgaga
    15541 agctacttag aattagtacc acaatatgca aaagtacata agaaaaaaaa tagaactaca
    15601 attttatgta ttattattgc agtatgctta gttactgcta tctttggact tgcagatatg
    15661 gctatacaat gtcaaaagat acaatttatt aaagatgatg gaaattggca catagtaatt
    15721 agaaatattg atgaaaagtt tatatcagaa ataaatagta gagtagatat agctcaccat
    15781 ggtagggggc aagtcgttga cagtggagag tttaaatcaa aggagatttt gatacaaggt
    15841 ttagatgaag aagtagcaaa acaatttaac ttagaaataa taaggggtcg ttatcctatt
    15901 ggggagaatg agactttaat agatgaggat gcagttaaaa gctttaatct aaaaatagga
    15961 gattttatca aaataaaatt agcagatggt agaaataaaa attataagat agtaggtgaa
    16021 tattcatcaa tatcaagctt aagagctaca gataaacatg gattaatact ttcaatattg
    16081 ggtacacaag ctattaaaaa atcagaaacg tacgattatt attatattac atttaaagaa
    16141 aaggttaata ttagaaaagc cacttctgat attaaaaata ccttcaattt aagtgataaa
    16201 caaatcggta aaaatgaaag attactttct attataggtc aaggcgactc tgacttttct
    16261 aaaccactgt atttaacagc aggattactt tttgtacttg ttttagtagc aagtattatc
    16321 atgatatata atagttttaa catgagtgta attgagagag ttaaattttt tggtctgtta
    16381 cgttgtctag ggtcatcaaa acatcaagtt aaaaaatttg ttgttttaga aagtttaatt
    16441 ttaagtataa aagctattcc aataggctta gtattaggaa gtcttgtaac tattatatct
    16501 tctatatttc ttaagtatgt taatgagtta ttcagctcaa tgccaatttt aaaagttagt
    16561 tttattggta ttgcatttgg aataattgta gggtttttaa ctgtaatatt atcagctatt
    16621 attccagcaa aaaaagcttc ccgtgtatct ccacttagcg ctgtacgtgg gaatctaatg
    16681 tccaaagaaa ttttaaataa cgggaagaca agtaaagttg caaatacagg aagttttaaa
    16741 attgagactt ctatgggatt aaatcatgct ttttcaagaa agaaatcttt tgtacttatg
    16801 acttcttcat ttgccattag tattatacta tttttaggtt ttagtgtgct tatagacctt
    16861 atgtatcaag gacttaagcc tatgaagcca gaagctcctc atatatctat tgttgaccct
    16921 aattttaagt ctgttttaac taaggaagat attaatgaaa taagcaatat caaaggagta
    16981 aagaaagtat acagtagaat gatgacggat atagaagcta catataatgg gaagaagaca
    17041 aaatcacaat taatttctta tgaaaagtat caatttaatt gggcaaaaaa atatctatta
    17101 aaaggagaag ttaatgaaaa taaactacag tcagaaaacc ttgcattagt agaagagggg
    17161 catggatttg atataggaga taagatatat attaaaaata gaaatgctga aaaagagtta
    17221 gaggtaggag gtatactttc ctcaattcca tttgaggcta aagataaata tattggaaat
    17281 attataacat ctgaaaatac ttttacgaat ataacaggta taaagggtta tataataatg
    17341 gatattcagg ttgataaaga tactcctgga gatttaattg aaaatataag acgtgtagta
    17401 gatgataact tagaggtcag agataaaaga caaagtaact tagaggttaa aaattcgttt
    17461 tatactatgg caacatttgt atatggattt gtgtttatta ttgcgattat aagcatgttt
    17521 aatatcataa atagcatgaa tataagtgtt acaagtagaa agaattacta tggtgttatg
    17581 cgtgcagtag gcatatccaa cagacaatta agaagaatgg taatagtaga ggcaggttca
    17641 tatgcaatga gtggttgttt attaggtagt atactaggat taatgttaca taggtatatg
    17701 tttatatcgt tagtaagttc aatgtttcat ttagagtggc aagtaccttt taatttacta
    17761 cttatcactg tactaactat gataataata actttattat cagtaagaaa accagtcaaa
    17821 aagatttgtg aattagatat aatagactca ataaatgcac aataaatata gctgttgatt
    17881 taaattaaaa aaacgaa
//
LOCUS       DAEPLF010000063        17463 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEPLF010000063 DAEPLF010000000
VERSION     DAEPLF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.063"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            88..738
                     /locus_tag="KR314_001804"
     CDS             88..738
                     /locus_tag="KR314_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBH0328190.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(878..2254)
                     /locus_tag="KR314_001805"
     CDS             complement(878..2254)
                     /locus_tag="KR314_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0328191.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2270..4183)
                     /locus_tag="KR314_001806"
     CDS             complement(2270..4183)
                     /locus_tag="KR314_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0328192.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4319..4951
                     /locus_tag="KR314_001807"
     CDS             4319..4951
                     /locus_tag="KR314_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBH0328193.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5090..5629
                     /locus_tag="KR314_001808"
     CDS             5090..5629
                     /locus_tag="KR314_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBH0328194.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAARKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(5805..5975)
                     /locus_tag="KR314_001809"
     CDS             complement(5805..5975)
                     /locus_tag="KR314_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0328195.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            6260..7327
                     /locus_tag="KR314_001810"
     CDS             6260..7327
                     /locus_tag="KR314_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBH0328196.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSIEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSSSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            7497..9398
                     /gene="cooS"
                     /locus_tag="KR314_001811"
     CDS             7497..9398
                     /gene="cooS"
                     /locus_tag="KR314_001811"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBH0328197.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            9391..9864
                     /locus_tag="KR314_001812"
     CDS             9391..9864
                     /locus_tag="KR314_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0328198.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            9839..11065
                     /locus_tag="KR314_001813"
     CDS             9839..11065
                     /locus_tag="KR314_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0328199.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFNIKENGEFCYSV"
     gene            complement(11204..12391)
                     /locus_tag="KR314_001814"
     CDS             complement(11204..12391)
                     /locus_tag="KR314_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0328200.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKSFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            12664..13488
                     /locus_tag="KR314_001815"
     CDS             12664..13488
                     /locus_tag="KR314_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBH0328201.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            13617..14882
                     /gene="gluD"
                     /locus_tag="KR314_001816"
     CDS             13617..14882
                     /gene="gluD"
                     /locus_tag="KR314_001816"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBH0328202.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            15075..15731
                     /locus_tag="KR314_001817"
     CDS             15075..15731
                     /locus_tag="KR314_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH0328203.1"
                     /translation="MYFLPPHDYKLEELDWIFGHELMYFKSKDLHLKYLVLFLKIVYW
                     FNPFIYIMEKYMDLDCELYCDERVLKNCTVQQKQEYALTIVNSMKRGINFSNKFVTGL
                     NKQSDIRKRVMNMFSEKHKNGILMALIISLFSSITFFKFDSTSLIEENDFLERSRYAF
                     EGRQEDMFVTIDSTYADAPIKLRKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK
                     "
     gene            16062..17195
                     /locus_tag="KR314_001818"
     CDS             16062..17195
                     /locus_tag="KR314_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBH0328204.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGVEYYDSLLND"
BASE COUNT         6717 a         2054 c         2839 g         5853 t
ORIGIN      
        1 gtaattgtat cttttattaa aaataatata aattaaaaca tataaataaa atattttata
       61 caatatattg taaaggaggt aactttgatg aaagcagaaa aaacaagtat atatccaatt
      121 attaaaaata atttaaacaa aatatcaatt gttgaatata aaaagggtca aagatttgta
      181 gcatcagata aagacttaca agaagtgttc tttataatag agggagttgc attagtggag
      241 tgtactacta gaggaggaaa taaatttctt gttgacattg tacctgaaaa tgaatttata
      301 ggcaaaatca gttacatata tgaacataat ttgaaatgtg atatatttgc aaagaccaat
      361 ataaaattat ttcgatttga aaaaaatgta tttgaagagt tttatctcaa accagatttt
      421 attgcactat ttcacagaaa atgtacaaga agaatatatg aattatataa aaccagaatg
      481 gtaagagaac tattttcttg cacagaagtt attgcctatt gtatatcaaa taaacaagaa
      541 aataatgtct gcaatataaa agctattcgt aatttcagtg aaatctattc aattagtaga
      601 aaaagtagat accattctct gctaaagtta actgaaaagg agatagtaaa aaaaatgggt
      661 aatacatatg aaattttaaa ctatgatgaa ttacataaat tggcatttga agtaaaagag
      721 tttttggaag atgagtaaaa attgttactt aatcaaaata aagtataata atttctgtaa
      781 aaaatagaaa tagtctcact gaaaatcaaa aatactttag ttattttcaa tgagactgtt
      841 tagaaatatt gtatacttat aatgtatagt ataattatta atcaatttcc tttactatat
      901 taatagccca ttttttagat aatgctctag gaacaccaaa tataaactta accacttctt
      961 cactataagt gagtaaaacg agtacaaata taggtgcacc tttaaatgca gctataaaag
     1021 ttagaggaat agaagctaac cacatacatc ctaattctag aaatagagca tatttagtat
     1081 ctcctccact tcttaaaata ccaattataa ccaatgtatt aaaagattta agagccatta
     1141 gtattcccat gataaagaat atcttagtta tatcagaagc taagctatca gaaacactaa
     1201 acatttttaa taataatggt atatttaaaa ttaaaataat tccaagtatt aaaccagctg
     1261 aaaatacaag tatagaaaac tttttagcat attcaatggc tcttttaata tggtcagctc
     1321 caagttcatt tccaagcata atagatgcac cagatgcaat acatacagct gtcattatga
     1381 agaaattacc tgtactggtt gctatctggc tagctgctat agcagaagta ccagctcttg
     1441 catatgcaac agaatacagg acagttccaa ttgcccatac agagtcattt acaaatatag
     1501 gaaagctttt tgatgaaaaa gattttataa aaattttatc aatagctttt agatttttta
     1561 gaccaaactt taatatatag tcttttttat aaaggtaaac ataagtaatc attaaaatta
     1621 attcacatat tctagcaata acagtagcta gagcagcacc ttttacaccc aatgcgggaa
     1681 gaccgaagtt accaaatata aatccataat ttaaaataac atttgttaca agtgcaaatg
     1741 cactacaaat catacctaac ttaggatttc taacacctct tgaaccagta ctaaatacag
     1801 tacttacagc aattaatgga taactaaata ctgcaatact aaagtaatct atacaaagtt
     1861 ttaccacctc agaatcatac gaaaatatgt gtataattgg ctttggtgtt agtaaagcag
     1921 gaataacaaa tacaaaactt aaaacaattg ccaatacaca agtaagacct gtgacttttc
     1981 ttatattact cacatccttc tttccataaa actgtgaaat aaaaactcca gcacctcctg
     2041 tgataccaga taaggacata ttaaaaagaa agaaaaattg gtttgcaact ccaactgaag
     2101 ctacagatgt ctcccccaaa ctacttatca taattgtatc tattacattt acagaagtag
     2161 aaattaagtt ctgtatgata ataggtatac ataaagatag aagtatttta taaaatcttt
     2221 tattttcgtc ttttaaagaa aatagttctt gcaaataatc ctctcctttt tacataaatt
     2281 cttcccactc atcatataga gaggcaaggt tattttctaa attaattttt tcttggctaa
     2341 cttctttagc tttatctgga ttagagtata cttcttcttg acataaaagt atatctaatt
     2401 tttcaatttc aatctctaac ttttcaattt ctttttctat attttgtctt tttattctat
     2461 ttttcttttc tatttctctt tgttctctct cttttctttt ttcatctttt atttgagttc
     2521 tagttttttc ttctttttct tctataatac tcatctcttg gatttgcctt tttttatcta
     2581 tataataatc ataatttccc agatattcag taattccatt ttcatctaaa actaaaattt
     2641 tatcaacaac agtatttaga aagtatctat catgtgatat tgtaaatata gtcccatcat
     2701 aattagttaa tgcctcttca aggacttcct tagaatcaat atctaggtga ttagtaggct
     2761 catctaataa caataagttt gcatttgata gtataagttt aagtatggca actctagctc
     2821 tttctccacc actcaatgta gaaatcttct taaaaacttc ttcatctaca aataaaaatg
     2881 cccctaacat atttctcaaa gtagtttgag taagatgagt attattttcc caaatctcat
     2941 caattattgt gttatctaaa ttaagagttt tttgttcctg atggaagtag gaaacatgta
     3001 cattagttcc aaatttaata ttgccactaa gaggttgtag ttcattagtt attattttaa
     3061 ataatgtaga tttgccaaca ccatttgcac ctataagtgc aactttttcc cctctatata
     3121 tatctaaatc caagtcttta aaaagtattc tttctccata acccatagat acatctctaa
     3181 tttgaagaac atcattacca ctctggacag aaggcgtaaa ttgtatacga gcttttttcc
     3241 tataagcttc tggtttatct aaaacgtcta ccttgtctaa tgctttttct ttacttcttg
     3301 cacgctttag gtgtttttct ctaccataag cttttagtct ttcaattgat tcttcttgct
     3361 tttttatttc tttttgttgg tcttcaaatt tcttaagctc tagttctttt tctattttag
     3421 ataactcaac aaatttagag tagttaccat tatatacttt aagttttttg ttgtgaactt
     3481 caaaaattct atttacggat tggtctaaga aatatctatc atgagatatt aacatgacag
     3541 ttcctttata ttgttttaaa aatacttcta accattctat tgcttcagag tctaagtggt
     3601 tagtaggttc atcaagtaac aatagagttg gtttcttaag aagtaatttt cctaaaagaa
     3661 ctctagtttt ttcaccacca gaaagtatgc ttattggctt atccatatca ttgtcactaa
     3721 atcctaatcc ttttagaact cctttggctt cagatttata tccatatcca tttaaatctg
     3781 aaaataattc tagtttatgt gaatattcat ccatgatttt ttctataatt ggagagtttg
     3841 tttttgtact ttcttcagat attttaacct ccaattctct taaataggat tccatttcta
     3901 tcaaattttt aaaaacttct agcacttctt ccaaaatagt attatccgaa taaaaattgg
     3961 tattttgttc taaatatccg atttcacaat ctttagaagt ataaatatct ccactatcat
     4021 atccataaat tcctgaaatt attttaaata aagtagtttt tccagtacca ttaacaccga
     4081 ttataccaat tttatcgcct tcatttactg taaaactaat attttctaat atagaatcta
     4141 taccaaaact tttatttaag ttattacatg acaaaacaat cataaatttc tacctccttg
     4201 aactatctaa aattataatg ttttgatata atataatcaa ggttatatgt attagttttg
     4261 aaaaagaaat attttattgt gaatcaaaca aaaaagagga cacttttagg aggtgaagat
     4321 gttgggaaat aaaaatatat caatggcagt tataagaagg ctcccaaaat atcatagata
     4381 tcttggagac ttattagata gggatataca aagaatatct tctaaagaat tgagtgatat
     4441 aatagggttt accgcttctc aaataagaca agatttaaac aactttggtg gatttggaca
     4501 acaaggatat ggttataatg tagaagctct tcatactgag ataggtaaaa ttcttgggtt
     4561 ggatcgacca tacaacgcag ttcttgtagg agcaggtaac ttaggacaag ctatagccaa
     4621 ttatgcagga tttagaaaag ctggattcga gataaaagct ttatttgatg caaatcctag
     4681 aatgataggt ttaaagataa gagagtttga agtattagat tcagatactt tagaagactt
     4741 tataaaaaac aataatatag atattgctgt attatgtata cctaaaaatg gagcacaaga
     4801 agttattaat agagttgtaa aagctggaat caaaggtgta tggaattttg cacctttaga
     4861 tttagaagtt ccgaaaggtg ttatagttga aaatgtaaac ttaacagaaa gtttatttac
     4921 cttatcgtat ttaatgaaag aaggaaagta gacaacttaa acatattaat aatgagcagt
     4981 tgtgaagagt attgagaaag tcagaagatt taatattaaa tcctctggct ttttgaaata
     5041 ttaggataaa aatttaaata atgtatggaa ataaacaata ttaaggagag tggtttttgt
     5101 ggaggaatta tggaaaaagg caattgaatt tcatggtcat gaatgtccag ggttagctat
     5161 aggatttaaa gcagcacttg cagcaagaaa atatttagga tgtaaacagt caggagatga
     5221 ggaaattgta tgtatttctg aaaatgatgc ttgtggaata gatggtatac aagtcgtgct
     5281 aagttgcaca atgggtaaag gaaatttact acttaggatg actgggaaat cagtatatca
     5341 tttctttaat agggaaaatg gaaaatctat aagaatatat gtaaagtcaa agccatttca
     5401 aatggataga gaaggataca aaaagtactt attagaaagt gattttgaag aaatatttga
     5461 gattacagat actaagttaa gacttcctga aaaagcaaga atatttaaat ctcttaaatg
     5521 tgaagtgtgt ggtgaatttg catctgagca atatataagg attgaagaag gaaagaaagt
     5581 atgtcttgat tgctttaata aatatgaaag attttatgag gagtattaaa attataaatt
     5641 agattaaagt taaaagtatt agatatgtac taacagtatt ttaagatgaa ttaggtagca
     5701 acataataaa atatataaat aatattactt gaaccataca taattaaaaa gaggctgatt
     5761 agtatttaga aaataaaatc agtctcttta tgaataacga attattattc tggttgaggt
     5821 gcatctacag gacaagctcc tgcacaagaa ccacagtcta tacaagaacc agcatctata
     5881 acatatgcgt cgtctccagc tgatatacag ctaacagggc attcagcctc acaagcacca
     5941 cagcttatac aagcgtcagt aattttatat gccattttga aatcctccct tttatatgta
     6001 ttgtaattca ataacattat aacaaatatt attgaatata acaattaaat taattctaca
     6061 acaaaaactt tattatgata ttcttgtttt aaagttttac aataaagttt aatctgctta
     6121 tatttttatt tattttaaag aagtttattt taaaaataaa attaaattta aaaagttatg
     6181 tattgattat atttaaaaat gttatactct aaaaaagtat attttatttt aaaatttatt
     6241 aatacaaagg agatttacta tgaaaaataa attagtttac atatgtatta tgagtctatt
     6301 gctatttgta gtaggatgtt ctaataatcc aaataaggaa agtaccatta aaaaagactc
     6361 taagaataac caaagccaag ttaaagaaga aaaaagagaa cctgtgaaaa aagatataac
     6421 tataagtttc gctggtgatg taactatggg gaactataaa ggttcatcat attatggcag
     6481 ttttgaccat gaatttaaaa gacaaggaga agattacaat tattttctta aaaatgtaaa
     6541 acctgtattt gaaaaagatg acttaacagt ggttaattta gaaggtccac ttactacagc
     6601 gtcaaatgca aaagaaaaaa aatttgcatt taaaggaaag cctggatatg taaatatttt
     6661 aaaatctgga agtatagagg cagtaagtat tgcaaataat cattcagaag attattttga
     6721 agaaggaatg aaagatacac agtttatatt ggatgagaat aaaataaatt attttggatt
     6781 agggaaagat gctgttgtag atgtcaaagg aattaaagtg ggtctattag ggtataatgg
     6841 attatcaact gaatataatg aaaaaaattt aaaacaaatg gaagatgata ttaagtcctt
     6901 aaaaaagaaa tcggatgttg ttgtagtgta ctttcattgg gggatagaat tagattatta
     6961 tccagataaa aaacaaaaag attttgctca ttatgctata gacaagggag cagatttagt
     7021 tatggggtct cacccacatg ttattcaagg aatagaaaag tataaaaata agtatatagc
     7081 ttatagtcta gggaattttt gctttggagg aaataagaat ccttctgata cagatagtta
     7141 tatatatcaa caaaccttta ctttttcaga taataaatta acttcaatta aagagccaaa
     7201 tataatacca acctcaataa catctagtag ctccagaaat aattatcaac caaaagtatt
     7261 ggatggaagt gagaaggata gagttttaaa taaacttgaa aaaataagca aagatttaaa
     7321 taaataaaat gtaaaaaaaa ataaatatat atatttattt taataattta tattttgttt
     7381 agggtatggt aaatcgtggt attaaataag tagattttaa tagttaaatt atagtcaatt
     7441 ataataaagc tatttaatct acttttttaa tataaaaata taagggggaa tttgtaatgg
     7501 cttgtaacac ttgtaaaatg tgcgaaagtg cagacaagaa attagaaagt tttgtagctt
     7561 caaaggatgt tgaaacagca tttcatagaa cagaagatca gaaagttaag tgtggttttg
     7621 gacttcaggg ggtttgttgt agactctgtt caaatggacc atgtagagta actcctaaat
     7681 caccaagagg tatatgtgga gcagatgcag acactatagt agcaagaaac tttttaagag
     7741 cagtagcatc aggagcagct tgttatttac atgtggtaga aaatacagct aagaatttaa
     7801 aaaatgttgg aataactaaa ggtgttgtta aaggagaaaa aacattaaat caattagcag
     7861 agatgtttgg aatagaaagt gatgaaaagt atgataaatg tataaaggtt gcagacaaag
     7921 ttatcaacga tttgtataaa tcaagagatg ataaaatgga attagtagaa aaaatagctt
     7981 atgcacctag agtgaaaaag tggaaagagt taggtataat gcctggtggt gcaaagtcag
     8041 aagtttttga tgcaatagta aaatcatcaa caaatttaaa tagtgatcca gtagacatgc
     8101 tagttaattg tttgaactta ggtatatcaa caggattata tggtcttaca ctaactaatc
     8161 ttttaaatga tgttatgttg ggagaaccag ttataagaat ggctcctgta ggatttaatg
     8221 taatagaccc agactacata aatataatga taacaggcca tcaacattca acatttgcaa
     8281 atttccaaga tagattaaaa gatgaagatg ttataaaatt agctcaatct gttggtgcaa
     8341 aaggctttaa gctagtagga tgtacatgtg ttggtcaaga tttacaatta agaggagaac
     8401 attatcaaga agtatttgct ggacatgcag gtaacaattt tacaagtgag gctgttttat
     8461 caactggagc aatagatata gttctttcag aatttaactg tactatacca gggcttgaac
     8521 caatagcaga taaatataaa gtaaaaatgg tttgtttaga tgatgttgct aaaaaagcga
     8581 atgcagaata cataggttta gacagaagta aattagatga acttagtaac actttaatag
     8641 aaaaagcatt ggagtcttat aaggagagaa gaggaagtat tgagatagat ataccaaaag
     8701 accatggatt tgaacaatct ttaactggtg taagtgaaaa gaacctgaaa gcgtttttgg
     8761 gagattcatg gaaacctctt attaatttaa ttgcagaagg aaaaattaaa ggtgttgcag
     8821 ctgttgtagg atgttcaaat atgacagcag gaggacatga tgtaaatact gttgaactta
     8881 ctaaagaatt gattaaaaaa gatataatag tactatctgc aggatgttct actggaggat
     8941 tagaaaatgt tggtcttatg tcacctggag cagaagaatt agcaggagaa aacttaaaag
     9001 aagtatgtaa gactttagga ataccaccag tattaaactt tggaccatgt ctagctatag
     9061 gaagactaga aatagtagca acagaattag cagctgaact tggaattgac ctaccacaat
     9121 taccattagt gctttcagct ccacaatggt tggaagaaca agcattagct gatggagcgt
     9181 ttggtcttgc ccttggctta cctcttcatt tagctttacc tccatttata acaggaggaa
     9241 aattggtgac agaagtatta acagaaaaat taaaagactt aacaggagga catgttatag
     9301 tgaatccaga ccctaaatct tcagctaatc aattagaaga aataataata gatagaagaa
     9361 aaaatttagg gttaaaggat gtggaatgca atgcatagaa tttttataaa taaagattta
     9421 tgtacaggat gtaagagctg tgtactagca tgtatgctaa aacacaataa agattatgac
     9481 atgtacactc ttgatttaga aaatatagat aatgatagta gagggcatat agaattagat
     9541 agtaaacata ataatccagt tccaattctt tgtagacact gtgatgaacc tgaatgtgta
     9601 ttagcatgta tgagtggtgc aatgcataaa gatagtgaaa gtggaatcgt ttcatatgat
     9661 gaagagaaat gtggttcatg ttatatgtgt gtaatgtctt gtccatatgg attattaaag
     9721 ccagatgata gaagcaagca aaacatactt aaatgtgatt tatgtaaaga tgaggagtat
     9781 cctagatgtg tagctaattg cccaagtgga gcaattgaat tacagaagga ggaaaatgat
     9841 gaattatgta gtgttagggg ctagtgcagc tggtataaat gcagtaaaaa cattaagaga
     9901 attagataaa gattcaaata tagtagtaat ttctaaagat gaaaacgtat attcaagatg
     9961 tatgttgcat cacgttatat ctgaacacag aactttaaaa caaataaatt ttgttgatga
    10021 agattttatg gagcaaaata atgtaaaatg gattgcaggt aagactgtta aaggtataga
    10081 cataaataaa aaggtagttc aaacagaaga cattactgta aattatgata aacttttaat
    10141 agctacaggt gcatcttctg ctattcctcc tataaaaaat ttaagagatg gaaattttgt
    10201 atattctgtg agaaacatag atgatattta taaaataaaa gaaaaagcac aaaattctaa
    10261 aaatgtagtt ataataggtg ctggtttagt tggtatagat gcattagtgg gattatttaa
    10321 atatgagcag ttaaatattt ctgtagcttt tatggaaaag tacattttag atagacaatt
    10381 ggatgaatat acagcatcag tgtatgaaaa taagttcaaa gaaaaaggtg taaaatttta
    10441 cccaagtgca tcaatacaag aaattgtatt ggataacagt aaaaatgtta caggtgttgc
    10501 attttcaaat ggtgaagttt tagatgcaga catggtaatt gtagctacag gggttaaacc
    10561 aaatgctgac tttttagatg ggacaggaat agaatatgat agaggtataa taatagatga
    10621 tatgtgccaa acaacacaaa aagacatata tgcagcagga gatgttgtag gtaaaaatgc
    10681 tatttggcct ctggcagtaa agcaaggaat tgtagctgcc tataatatgg ttggaaaaga
    10741 taagaagata gaagatgaat ttgcttttaa aaatagtatg aatttcatgg atataccaac
    10801 tatttctata ggaatgaata cacctgtaga tgatagctat aaagtattaa ccagacatgg
    10861 aattaatgat tataagaagt ttgtttttaa agataatgta atctacggag cagttataca
    10921 aggagatatt tcatatgtag gtgtattaac ttatttaata aaaaataaag ttgaaatata
    10981 tgatttagaa aataggatat ttgatgtatg ttatgcagac ttttttaata taaaagaaaa
    11041 tggagaattt tgttatagtg tatagtatat acgtggagta aggtttataa atacagtaaa
    11101 tcaataaata gagttattct aaaataagaa aaatgacaaa tcttatttag aaatagctct
    11161 attttttgta attaaattta aattatattc aatttgtaaa atattatttt gaacaattat
    11221 acatatactc tctagttaga ggtaaatcat tttttatacc tttagtgaaa agtatttgat
    11281 gtatatctat aataccagtg ttgaaagcag cagcacaaga acaaaggtat aaatcccaca
    11341 ttcttataaa cttatcatca aacatttctt taatctttgg aagattctca gagaaacttt
    11401 tttgccagta tagtaaggtt tttacataat gtaatcttaa actttctacg tctatagtat
    11461 gatatttgta atcactacaa atatgtatca tttctcttag gctaggaata actcctcctg
    11521 ggaaaatata tttttttatc caagggtctc cataagattc ctgcaatcca cttatatagt
    11581 gaagtaaaaa caatccacca tcttttaaaa cagcatttac gtttttaatg aaaagttcat
    11641 agttttctct accaacatgt tcaagcattc caacgcttac aactctatca aacataaggt
    11701 ctgatttttt taattctcta tagtccataa gtcttacctc aagatagtct tgaaggttct
    11761 cttctttaat tcttgcgtta aattccttaa attgctcttc gctaagtgta attccaacgc
    11821 cgtgaatttt atattttttt gcagcttcta ttagtaaaaa tccccaacca caaccaatat
    11881 cgagaaggct cataccttcc tctaaattta gtttagttaa tatgtggtga actttattga
    11941 cttgagcatc ataaagagta tcattatcat ttctaaagta tgcacaagaa taactaagtg
    12001 tcttatctaa ccataagcta taaaaatcgt tgccaatgtc atagtgggca tgtatttctt
    12061 tcttctgatg tctcattgaa tttgatgagt gcaatagatg ttttacagct gaattatcaa
    12121 tattgaattt gtctatttgt tttaaaagta gatttaaagt taagaataaa tctccctcaa
    12181 cttcaatgtc tctatttatg taggcttctc caagtgctaa agatgtactt tttaatagtt
    12241 cagacttgct aatatcatta tttactataa ctttgaactg aggggagccc tctccaatta
    12301 gaaattcttc ctcatcttta agcctaacta gaaatggtgt ttcaccaaat ttctctaaat
    12361 agtcttttaa aaaatttttt gtaatattca tattaccagc tccttctaat gtaaatccag
    12421 taagtttccc tgcttaaaat atattatagt ccttttttta aaaaagtaaa attttttcaa
    12481 taaatttttt atttaaagga aatttatttt ttataaagaa atttcatgtt gtagtatatt
    12541 ttttgcttat aaattttggt actggatata atattaaact gttaatgata taataaattt
    12601 aatctataaa gtaaataaaa ttttgtaata aatagttaaa gctattttat aaggagatat
    12661 atgatggaag tatatgcttt ggttgggtca agtggcacag ggaagagctt taaggcttta
    12721 gagttcgcat atgaaaatga tatagaatat ataatcgatg atgggatttt aatatacaaa
    12781 aacaaggttt tagctgggat atcggcaaaa caagcaaata ctactataga agcagttaaa
    12841 agggctattt tttataatct ggagcataga caagaagtta gagaagcaat taaaaaagaa
    12901 aatataagaa aaattcttat tattgggaca tcaaaaaaaa tggttaatca aattgtagag
    12961 agattgtcaa ttggaagggt ctgtaagttt ataaatatac aagatataag tactaaatat
    13021 gaaatagaaa tagcaaaaca agctagaaaa gaaggaaatc atgtaattcc tgttccagcc
    13081 gttgagataa aaagtatggc aagtggtctc agtataaatt ctttgaagag actttttaga
    13141 aaaggaaata ataggaatat ggtagtttta gaaaaaacaa ttataagacc aacttttagt
    13201 tatataggaa agttttatat tagtgctgat gtaattaaac agattattga atatgaagta
    13261 tacaactttg gaagcattga tagaataaat aaaatcaata tagagaattg caataatttt
    13321 atgaatattt ttgtaagtat aaatatcaat gatttagaaa ctataaaaaa aattgaagac
    13381 atacaacaga caataaaaaa gtctattgag aaaatgactt tagttaatgt acaaaaaata
    13441 gatattaaca ttcataaaat taaaaaaata aacttcctcc aatgttaaaa aataaactga
    13501 gaaaatgata tactaatttg caataaagtg tgctataatt aaactgtaaa tttaggaaat
    13561 ggttttttct aaatggagta tacataatta taacgtattt ataagggggc tttcaaatgt
    13621 caggaaaaga tgtaaatgtc ttcgagatgg cgcaatctca agtaaaaaat gcatgtgata
    13681 aattaggtat ggaaccagca gtttatgaat tattaaaaga acctatgaga gttatagagg
    13741 tttcaattcc agtaaaaatg gatgatggtt ctataaaaac ttttaaagga tttagatcac
    13801 aacataatga tgcagtaggg ccaacaaaag gtggaataag attccatcaa aatgtttcaa
    13861 gagacgaagt aaaagcttta tctatatgga tgactttcaa gtgttctgta acaggtatac
    13921 catatggtgg aggtaaaggt ggaataatag tagatccatc aactttatct caaggtgaat
    13981 tagaaagatt aagtagagga tacatagatg gaatatataa attaataggt gaaaaagttg
    14041 acgttcctgc accagacgta aatactaatg gtcaaataat gtcttggatg gttgatgagt
    14101 acaataaatt aactggacaa agttctatag gtgttataac tggtaagcca gttgaatttg
    14161 gtggttcttt aggaagaaca gctgctactg gatttggtgt tgctgttact gcaagagaag
    14221 ctgctgctaa attaggaata gacatgaaaa aagctaaaat agctgttcaa ggtataggaa
    14281 acgttggttc ttatacagtt cttaactgtg aaaaacttgg tggtactgtt gtagctatgg
    14341 ctgaatggtg taaatcagaa ggttcttatg ctatatacaa tgaaaatggt ttagatggtc
    14401 aagctatgtt agattatatg aaagaacatg gtaacttatt aaacttccca ggagctaaga
    14461 gaatatcttt agaagagttc tgggcttcag atgttgatat agtaatacca gctgcattag
    14521 aaaactctat aactaaagaa gttgctgaat ctataaaagc taaattagtt tgtgaggctg
    14581 ctaatggacc aactactcca gaggctgacg aagtatttgc tgaaagagga atagttctta
    14641 ctccagatat attaactaat gctggtggag ttacagtttc ttacttcgag tgggtacaaa
    14701 acttatatgg atactactgg tcagaagaag aagtagagca aaaagaagaa atagctatgg
    14761 ttaaagcatt tgaatctatt tggaaaatca aagaagagta caatgttaca atgagagaag
    14821 ctgcttacat gcattcaatt aagaaagttg ctgaagctat gaaattaaga ggatggtact
    14881 aagatttact cttaataaat aataggatat aattatttaa gatataaagg ggctgtacat
    14941 agtacagccc ttaaattatg tggcttacaa tgaagtaagc tatttttgtt ttaacttata
    15001 aataacttga taaattatgt aatttagaat gcataaataa gatgggtgaa aattaatggt
    15061 tgatttaatt ttgaatgtat tttttaccac cacatgatta caaattagag gagttagatt
    15121 ggatttttgg acatgaactt atgtatttta aaagcaaaga ccttcattta aagtatctag
    15181 tattattttt aaaaatagta tattggttta atccatttat atatataatg gaaaaatata
    15241 tggatttgga ttgtgaactt tattgtgatg agagagtttt aaaaaattgt actgtacaac
    15301 aaaagcaaga atatgctcta actattgtaa attcaatgaa aagaggtata aatttttcaa
    15361 ataaatttgt aactggatta aataaacaat ctgatattag aaaaagagta atgaacatgt
    15421 tcagtgaaaa acataaaaat ggtatcttaa tggctttgat tataagcttg ttttcttcta
    15481 ttacattttt taaattcgat tcaactagtc ttattgaaga gaatgatttc ttagagaggt
    15541 caagatatgc gtttgaagga aggcaagaag acatgtttgt aacaatagat tctacatatg
    15601 cagatgcacc tataaaactt agaaaagagc atgaagaaaa ctgtagagtc aatggaatag
    15661 taccaaaaga ttcagattca atagaaatta ttcctagatt ttataaagat tgtatggatt
    15721 taaaaaaata agttagactt aatattgatg ttgatattat gaatttttta tatttaagca
    15781 attgaaaatt ttattataga ttcaatttta tatgtgttta agtatgaaat ttatagatag
    15841 ttgaaataat taaatatgat tgatgcatag gaatcattac taagctataa ttaattatag
    15901 gggagtttaa ataaattatg agttacacaa ttataaaatg ataaaaaata gttatctgtt
    15961 acaaaaaatg tatcttttta attaataaag ataatatgaa aacttaaaag gcaaataaac
    16021 ttaaacataa aatactctta aataagatag gagaaaaatg tatgtctaac tttattctaa
    16081 atgggatttt tagaacagtt atagtaagta gtctaagcat tcttctgata ttgattttca
    16141 agaaaaatgt atttaaaaga tttagcaaaa aatttaatta ttatatatgg atgatagttg
    16201 ttattaaatt atttttacca tttacatatt atacttttac aataaatatc ttgagaagta
    16261 aaaaacacat taatattaat aacataaatt tagagggatt taataatgtt tcaactataa
    16321 gtaataatat tatattgtac acttggatta taactgtagt agtgtattta atttacacta
    16381 tatttaaata tataaaactt aaaaatttaa taaatgactt atcttatgat gttgatgata
    16441 gagagattgt aaatctttat aaaaatatat tagaagaatt taatataaca aaagatataa
    16501 aactcaaata ctcttatgaa gttgaaacac cagcattttt taattcatgt gtacttttgc
    16561 caccacatga gtataaattg aaagagttag actggatttt tagacatgag cttatgcatt
    16621 ttaagagtag ggacctttat ttaaaatatg taatcctgtt tttaaaaaca gtatattggt
    16681 ttaatccatt tatatatata atggataaac atatagattt agattgtgaa ctttattgtg
    16741 atgaaagagt tttaaaaaat cgaaattcag aggaaaaaaa ggattatgct ttaacaattc
    16801 taaatgcaat gagaaaaggt tcaaatactt caaataaatt tatagctgga ctccataagc
    16861 aatctgatat taaaaagaga gttatgaata tgtttaatga aaagtacaag aatggtatat
    16921 taatggcatt aactttatgt ttattatctt ctattacttt tttgaaggtt aattcaacga
    16981 gtattgttaa tcttaatgta ttacctaaag gatttgaaga cttacgaact gttagcagta
    17041 tacgtttttc agttatagga tttacatatg cagatgctcc taaaaatatg agggaagaat
    17101 ataaaataaa ttgtgaatca ttaggtttga tacctaaaga ctctgataaa atagaagttg
    17161 gtgtagaata ttatgacagt ctattgaatg attaaataaa tattatttta acagatatta
    17221 attataaagt tttgaatagt aatttgttga gaaataaata tatccttata aaaatagata
    17281 cttatatacc aagaataaaa aatagttaag atagtacaca aggatatact tataaagaac
    17341 ttaaataagt gaaataaatt attaattata aggtttataa ttttaaaaaa ttagataaat
    17401 tcaggtgtaa aatactataa aacacaattc ggaaaagtgt atgttgaact tcatttaaaa
    17461 tag
//
LOCUS       DAEPLF010000064        17352 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEPLF010000064 DAEPLF010000000
VERSION     DAEPLF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.064"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..652
                     /locus_tag="KR314_001819"
     CDS             <1..652
                     /locus_tag="KR314_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBH0328205.1"
                     /translation="SSIIVYKSLIFKDDIYVVSKKNLPNEKTHVVENEIKGEDNNHNK
                     VTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAVELLGGTTKEADLNGVNLS
                     VRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPKNKSNKNISNESKVNINVA
                     TIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNGIGDKKYENIKDLICVD"
     gene            744..1790
                     /gene="selD"
                     /locus_tag="KR314_001820"
     CDS             744..1790
                     /gene="selD"
                     /locus_tag="KR314_001820"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:792..794,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBH0328206.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDNPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            1808..3220
                     /locus_tag="KR314_001821"
     CDS             1808..3220
                     /locus_tag="KR314_001821"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBH0328207.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVAELRKAFN"
     gene            3334..5217
                     /gene="selB"
                     /locus_tag="KR314_001822"
     CDS             3334..5217
                     /gene="selB"
                     /locus_tag="KR314_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBH0328208.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALEALISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            5322..8060
                     /locus_tag="KR314_001823"
     CDS             5322..8060
                     /locus_tag="KR314_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBH0328209.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSIILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(8179..9123)
                     /gene="manA"
                     /locus_tag="KR314_001824"
     CDS             complement(8179..9123)
                     /gene="manA"
                     /locus_tag="KR314_001824"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBH0328210.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            9296..10657
                     /locus_tag="KR314_001825"
     CDS             9296..10657
                     /locus_tag="KR314_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0328211.1"
                     /translation="MELFKAESKKYEKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSIVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            10745..12634
                     /locus_tag="KR314_001826"
     CDS             10745..12634
                     /locus_tag="KR314_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0328212.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIYCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            12649..13104
                     /locus_tag="KR314_001827"
     CDS             12649..13104
                     /locus_tag="KR314_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0328213.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            13163..13480
                     /locus_tag="KR314_001828"
     CDS             13163..13480
                     /locus_tag="KR314_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBH0328214.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            13505..14767
                     /locus_tag="KR314_001829"
     CDS             13505..14767
                     /locus_tag="KR314_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBH0328215.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFIDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            14872..15936
                     /locus_tag="KR314_001830"
     CDS             14872..15936
                     /locus_tag="KR314_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0328216.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKIGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKIIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            15990..17168
                     /locus_tag="KR314_001831"
     CDS             15990..17168
                     /locus_tag="KR314_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBH0328217.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKNIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
BASE COUNT         7065 a         1731 c         3101 g         5455 t
ORIGIN      
        1 atcatcaata atcgtttata aatcgcttat tttcaaagat gatatttatg ttgtaagtaa
       61 aaaaaattta cctaatgaaa aaacacatgt tgtagagaat gaaattaaag gtgaagataa
      121 taatcacaat aaagtaacta aagacataga taaaaatttt caaaataata aaataactat
      181 atacataagt ggagcagtaa atagacctgg aattgtaact atagagtcag acaagagact
      241 ttatgatgct gtagaattac taggaggaac gacaaaagaa gcagatttaa atggagtcaa
      301 tctatcagta agattagaag atgaacaaca ttacataata cctaaaatag gtgaagcaac
      361 atctgttaca agtaatgatg attccaataa accaaatcag aagaatgagt caagaggtga
      421 gcctaaaaat aaatccaata aaaatatatc taacgaatca aaagtaaata taaatgtagc
      481 gactatagaa gagttagatt ctctacctgg ggttggagaa gctacagcca ataaaatttt
      541 acaacacaga gaagaaaatg gacagtttag ttcaattgag gaaataaaaa atgtaaatgg
      601 tattggagat aaaaagtatg aaaatataaa agatttaatt tgtgtagact aaaatataat
      661 acaaaactta catatagata tgttattata aatatgtatg tatatgtgtg tacatgtgga
      721 aatttagtaa aggaggcact tttatgaaaa aagaacaaaa aaaacttaca gagatgacaa
      781 cagcaggtgg ttgagccgct aaaatagggc cagaggttct ggcatcagtt ttatctcaat
      841 tgcctaaaaa tgataatata gaaaatttat tagtaggact agatacagca gatgatgctg
      901 cagtttataa attaaatgat gatatggcac ttatccaaac tttagacttc tttacaccaa
      961 tggtagataa cccatacgta tttggtcaaa ttgctgcatc aaattcacta tcagatgtat
     1021 atgctatggg aggcaaacct ttggttgcta tgaatattgt ttgttttcca tcatgccatg
     1081 atatggatgt acttgcggaa atattaaaag gtgggtttga taaagtgaaa gaaagtggag
     1141 cacttttggt tggagggcat actgtagatg acaaagagcc taaatatggt ctttcagttt
     1201 ctgggattgt aagtccaaat aaagttttat caaatgctac tgcaaaacct ggtgataagt
     1261 taattataac aaaaccaatt ggagtgggtg tattaaatac agccatgaaa gaaggtatgg
     1321 tagaacaaca tatagcagat aaagtaatag aaataatgat acatttaaat aaatatgcag
     1381 caatgagttt tgagaaattt gatgtaaatt cggttacaga tataactggt tttggtctac
     1441 ttggtcatac tttagaaatg gccaaagctt ctgaagtaag tattgaaatt gaatcaaaac
     1501 atgtaccaat tattgaaggt gcaatagaaa tggcacaaat gggaattata cctgctggta
     1561 tgtataaaaa tatgcattat gtatctaaag atgtagaagt tgtaggaaat atagaagttg
     1621 cagttcaaga tattttatac gacccacaaa catcaggagg gttgttaatt tcagtaaaag
     1681 aagaacttgc agaggaactt gtaaaagata tgaaattaaa tggagcaata gaagctaaaa
     1741 ttataggttc tgtagttcca aaaggggaaa aatatattac agttttataa aatattatag
     1801 agaaagagtg attgttttga gcaaaagaga attgtttgca atgttgcctt cagttgatga
     1861 agtattaagt gataatagaa ttgtagaaat aataaatgaa tatcctagaa gtttagtttt
     1921 agaatctgtg agagaagtaa tagatttaaa aagacaattt atattaagat taaaagaaga
     1981 tgcatcaaac tctgtaacaa tagagtttgg tgaaataata gaatctgcaa tagagagagt
     2041 taagttaaat tactcattat ctttaaaaaa agttataaat gcaactggta cagttattca
     2101 tacaaattta gggaggtctt tacttagtga agatataaaa gatgaattat ggtgtgctgc
     2161 ttcaaggtat tcaaacttag aatacgattt ggataatgga gaaagaggtt caagatactc
     2221 tcatttaact agtacaataa aaagacttac tggagcagaa gatgtactag ttgtaaataa
     2281 taatgcagcg gcagtacttc ttgtacttag cacaatggct aaaggaggag aagcaattgt
     2341 atctcgtgga gaattagttg aagtaggtgg ttcatttagg attccaagta taatggctct
     2401 tagtggtgca gaattagttg aagttggttc aactaataaa actcatttga aagattacaa
     2461 ggaggccatt acagaagata caaatgtatt gatgaaggtt catacaagta attatagaat
     2521 aatgggattc acagagagtg tttctataga agaattggtt aatttaggta aaaaatataa
     2581 actacctgta atagaagact taggaagtgg agtttttata gatttatcaa aatacggttt
     2641 atcttatgaa ccaactgtat tggattctat tcgacaaggt gcagatgtag taacttttag
     2701 tggagataag atgcttggag gtccacaagc aggtattata gttgggaaaa aagagtatat
     2761 agaaaaaatg aagaaaaatc aacttactag agcacttaga gttgataagc ttacaatatg
     2821 tgctcttgaa gcaacattga gaatgtattt agatgagaca aaagctatag aaaatatacc
     2881 tactttaaaa atgttgacat ataaaattga agaattagaa gtaaaagcaa ataaactatt
     2941 tgaaaaaata actgctttaa acttaaatgc aaacataaat atagaagatg gattttcaca
     3001 agtgggagga gggtctatgc ctcttgaaac tataagtaca aaggtaatat caataactcc
     3061 tgaacatatg aatgtttcat ctttagaaaa aaaactaaga cttagtgaag cacatataat
     3121 agccagagtg tatgataata aatatgtact agatgtaaga actatatttg atgatgaatt
     3181 tgatgtaata gttgcggaat tgagaaaagc atttaattaa ataaatttga gctaataaat
     3241 attaaattta gaagtataag tatgaagttt tgattaaaaa atagctacac acacttgtga
     3301 ctttagtcat agcaggttta gagggggcaa aaaatgaaaa atgtagtaat tggaacagca
     3361 ggtcatattg accacggaaa aactactctt ataaaagcat tgactggaag agagactgat
     3421 acattagatg aagaaaaaaa gagaggtatt tcaataaact taggatttac ttattttgat
     3481 ttaccaaaca atagaagagc tggaattgtt gatgtcccag gacacgaaaa attcataaaa
     3541 aacatgttag ctggagcatc aggaattgat atagttttac ttgttgtagc agcagatgaa
     3601 ggggttatgc cacagacagt tgagcattta gatattctaa gctttttaaa tatcaaaaat
     3661 ggaatagtgg ttctaactaa gtctgatact gtagatgatg aatttagaga gctagtaaaa
     3721 gaagatattt gtgaaaaaat aaaaggaact tttcttgaag atgctgaaat aattgaagtt
     3781 gattctatat ctaaaagagg tatagacact cttattgaaa aaatagataa aatgagtgat
     3841 gatatagaag ataaaaatga aaactctcca gcaagactta atatagacag agtattttca
     3901 ataaaagggt ttggtactgt aataacaggc actttaatag aaggaaagat aagtatagaa
     3961 gatgatttag ttatatatcc acaggtatta aaaacaaaaa taagaagtat acaagttcat
     4021 ggagaaaata aagatacagc ttatgcaggt caaagaacag caatcaatat atcaaatgta
     4081 aaagtggatg atataaaaag aggagatgtt ttagctgcac caaactcatt agaggaatca
     4141 atgatgttag atgtaaaatt atcattggta aatcatttag ataaaggatt aaagcactgg
     4201 gatagattaa gattgtatca tggaactaga gagatacttt gtagagcagt tccactagat
     4261 aaagaattaa ttgaaagtgg agaaagtggc tatgttcagt tgagacttga agaaagcata
     4321 gtatctaaga aaggtgatac gtttgtagtg agacgttatt ctccaatgga aactataggt
     4381 ggaggtgtaa taattgaccc atctcctaaa aaacataaga aatttgatga aaaagttata
     4441 gaagcattaa aaataaaaga aaaaggcgaa ctaaaagata taatagaaga gtacttaaaa
     4501 aggaatttaa aaaattatcc aaatataaaa gaaataatga gctatagtgg tgctcatgaa
     4561 gaagatgtaa aaagagcgct tgaagcactt atatcagaag ataaagtatt tattatagga
     4621 aatatgtata tgcatataaa tcaatacaat aaattaaaag aaaatacaat aaagctttta
     4681 agtgagtacc ataagaagta taggcttaga aaagggatac taaaagaaga agtaaggtct
     4741 aagatagaga gtaattttaa gactagggag atggatattt tattagaaaa attgtctaca
     4801 gaaaatgcta taaaaataga aaataatata gtatctctat tagattttga ggttatttta
     4861 aatgataagc aaaaagaaat agcaaaaaaa atagaaaaaa gattaaaatc atgtggagtt
     4921 tcttctatac tgacaataga tgaagtttct gaaggaaatc ataactatgc tgaagtattg
     4981 gagtcaatga taggtaataa agtagagaaa ttagatgatt tgtatataat ggacaaagat
     5041 atttatgaaa atgcaaagaa cattttgata aattatataa aagaaaataa agaaataaca
     5101 ttaggagaat acagagattt gattgattct agcagaaaga attgcatgat tatattggaa
     5161 aacttcgata gaaacaaaat aactaaaaga gtagaaaaca aaagaatttt gttttagcag
     5221 aaaatgtctg tttttgcttt aaggtataaa gaaggaatta taatgtcgag tatcgtataa
     5281 ttaattagta taaaatatta attaagagga agtgtaaagt attgaagaat ataatacctg
     5341 atgtaaaacc agatataaaa gaaagagaag acatgagcct tttatttata ataatgggct
     5401 cagtactttt attatttatt ataaaagata tagagaaata tgaaacttta ttacatgcca
     5461 tatatacgat atttttagta gctacaggag taattatatt taatactaca aaatttaaag
     5521 tagattcatt ttctacattt ttaggattaa tttttatagc tactggagta ttggagtgta
     5581 tatatttatt taattccttg gggattaaaa ctaagtctat aatggagatt aacatcacaa
     5641 tatcagcaat aacagattta tttcctatac tgggtgtata cttaagtttt aaatttgtta
     5701 aggataacaa acaaatttat tcaagtataa ttttatttat agcaactgct acattaacaa
     5761 taacatcttt attcataata tcaggaattt gtgactatct gggaaatggt aaatcacaat
     5821 atgctttagg agttataata tctatattca taataattat ttctatcatt agtggaattg
     5881 aattaaattc aagttcaaat aaaggtaaat gggaatatga tgaaaaaaag atttttaaca
     5941 gaataatagt aataataatt ttatcaagag ttccaaatct attgcatata gttattgata
     6001 atagaaatgt agagcggata ttatcacaga taataataaa tgtagcctta tattatttat
     6061 ataattacat agtatctaag aatattaaga aaacagcact ggaattgcat gatacgaatg
     6121 aagaattaac aaaaaaaacg gaaagtttaa aagaaaagaa taaaaaatta atctatgaga
     6181 cagaaaagat agaggaactt aagggaatac ttatgaaaag agaatctagg ctacaatcta
     6241 cattagatgt agcagtaaat agcatagtag tgtttagcaa agatggagaa attacatatg
     6301 caaataagag ctttagaaat acttttggag actataaaga acaagataaa ttgactgata
     6361 aaataaaaaa cttcaatgat ttgatagaaa gcattcatta tgtgtttgtt catgagaaga
     6421 atgcagagaa acttattagt acaagcgata ataaagttta tcaagctata tttgcacctt
     6481 taattattgc ttcacaaaca gaaggagtat tgtgtgtact agaagataag actaagaaaa
     6541 aggagtacga acgcaaactt attgaagcaa ataagagaag taaagatttt ttagaaagta
     6601 ttggagatgg catagtagtg cttgaaggta ataacaaaat atatgttaat aatgcatgta
     6661 gggaaatttt taaaaataat ttagagagta ttgatttttc attagtatgt aaaagtgaag
     6721 aaagtataga aaaaagatat gtaatagatg gtgaagtaaa gtatgtagag atgagttttt
     6781 ctcagtatac taatgctggt actaataaaa ctattattgt aatacgtgat acaacctctc
     6841 gaaaaatatc tcaaataaaa cttgaggaaa gtcaatcatc atattcaaga tttatagata
     6901 tattacctga tggcatatgc ttattaaaag aagatttaag tataaactat gcaaataagt
     6961 cgtttttaga tatgctagct tttacaaaca tagatgatat aaaagattct aatattaaac
     7021 tattaatgaa tgcgaatact gaagaaaaaa tgaaatttac ggataatatg gctaaagttt
     7081 taaatgaaaa tagaagtata cttttactgg aatatgaatt gataaatagt tatgatgata
     7141 tagtagaagt agaattaagt gcattacctt ttgctatata caatactaga tacataatgc
     7201 ttattataaa agatttagta cataagaaat attccgagca agcagaaaaa gaactgcttg
     7261 aaagatttaa aacagataaa atcaaaacgg agttttttgc aaatatgtct catgaattaa
     7321 aaactccact aaatgttata tctagttcaa atcaactggt agattctttc tatagaaatg
     7381 aaaagataaa agattacaat aacaatatta aatctcatgt ggatttagtt agacaaagtt
     7441 cttataggct tcaaagatta ataaataaca ttatagattt aacaaagatg gagtctgggt
     7501 tctatacact aaaacttgcg aaatacaata ttgtatctgt aatagaagac ttatttatga
     7561 atatagaaga gtatgcactt agaaaagaca taaaaattct ttttgataca gaccttgaag
     7621 aaattaatgt ttatatagac aaggttgaaa ttgagagaat tatgttgaat ctattgtcaa
     7681 actgtattaa gtttactgat aatggaggct ggatatatgt aagtattcat tataaaatag
     7741 ataaagtaat cataagtgta aaagacactg gagttggaat accacaagat aaattagaat
     7801 tgatttttga agaattttca caagttgata agactttatc cagaaatact gaaggaagtg
     7861 gaatagggct tgctatagtt aagaatctag taagtcttca tggtggagat atagaagtag
     7921 taagtgaagt gaataaaggc acagagttct taatttcact tccaatgaag ggatttagta
     7981 gtgaacatta cacagaagat aagagaatat ataatattca agaaaaaata aaaatagaat
     8041 tttctgatat atactattaa aaatgattat gagcaaaaat aaaataaaaa tactatttgt
     8101 ttaaatattt aagttgtaag aaataggcta tcataaggta aattattatg atagcctatt
     8161 tttagctagg ttttatatct atatgtgaga acatataatc tctaaattac cttcaaaact
     8221 aaaatctcca aagtcgtttg gcagtataaa atgcataccc ttttttaaat tatattctac
     8281 agaattgtgt ataagttttc catttccatc caacacactg tataaagaaa aaggtgtatt
     8341 gtgtgtaaaa ttacattttc caaaaacatc taatttataa acactaaaaa attcgtttga
     8401 cacaaatgtt gtgcatttaa agtctgatgt tgatacgatt ttatagtcag tatcgaaatt
     8461 tatatgagga acattcgtaa catctattga tttttgaaca tgaagttcac gcttattacc
     8521 tgaattgtca gttctatcat aatcatagac tctataagta gtatctgagt tttgttgagt
     8581 ttctaaaatc aatgttcctt tgcatatagc atgtatagta ccacttggaa catagaaaaa
     8641 gtctcccttt tttatattaa cctttctaag tagtttgtcc cattcattat tatttaccat
     8701 atttacaaat tctttatgag attttgcatt atgacctatt ataatttctg catcatcaga
     8761 acaatctatt acataccaac actctgtctt acctaactct ccattttcat ttttcttagc
     8821 atattcatca tttggatgga cttgaactga taaattatca tttgcatcca atatttttgt
     8881 aagtaaagga aacttatcac caggtgtgtt tccaaataat tctctatttt tattccacaa
     8941 ttcagatagt tttttacctt tatattttcc attttctatt aagcaatctc catttttatg
     9001 agagcttata gcccaacatt caccagttgt tggcgaatct atttcataat taaatttatc
     9061 tttaagtgca gtaccacccc atattctatc cataaaaata ggttttaaaa ataatggttc
     9121 cataatcctg ttcctcctat gatattaaat ttatcctaca aataattata tataaataga
     9181 ttataaaatc aatagtaaaa aagttttgtc ccttgaaatg acataaagta taaggagtta
     9241 ttaaataaga taaactatga tatataatat aaagttctaa attggagtga taattatgga
     9301 gttgtttaag gcagaaagca aaaaatatga aaaagaattt ttaaaattat tggagcaatg
     9361 ggttagtata ccttcatttt atgatagaaa aactgtatct aaagatatgc cttttggaaa
     9421 aggagtatat gatgcattaa actggtttga aaatctaggt agagaaaaca atttcaaagt
     9481 aaaaaatata gataatcatg ctgttcaaat tgaatatgga aatggtaaag aatatgtaga
     9541 tatatttgga cattgtgatg ttgtaaatcc aggtgaagga tgggatagtg aaccatttaa
     9601 actaaatatt ataggtgata aattagttgc tagaggtgta tctgataata aaggacctat
     9661 gatagtgaat tttttagcac taaaaatgat aaaggattta gatattaatt taaaaagaaa
     9721 ggttagatta atagctggag gaaatgaaga gagtggattt aaatgtataa agcattatta
     9781 tagcaaagag ccatatggtg tatgtggatt tactccagat gctaaatttc ctgtattaaa
     9841 tggagaaaaa ggtggagcaa taataaagtt aatttcaaat atagacgata aatctttata
     9901 tataagtggt ggaatagagt ttaatacaat accagataaa gtttatataa agaatgtaga
     9961 aaaattgggt aaagataata tatgttttga tattaacaat atatcgataa attatgataa
    10021 tggcaattat attgtgtatg gaaaaggtgg tcattcatct aaacccgaaa aatcaataaa
    10081 tcctatatta gctacaatta aacttttgtc agaaaatatt gatgaaaaat ggacaaaaga
    10141 tttatataag ttgataaatc aagataatat aaatggaaac ttatttggtt taaatattga
    10201 ggggaaatgt gggattttat caatagtgcc aacaataata aatatagtag atggaaaatt
    10261 agaagtagtt ttaagcgtta gatatccaga aatattaacc attgaagata taataaaaaa
    10321 atttaatttg tatatggagc aaaataatat taataaattt gagcttattg gggaaaattt
    10381 aaagcaagca aattatatag atagaaattc aaaacttgta agaagtttgc atgatatata
    10441 tataaagtat tctggagatt tgaaaaatga tgttagagta actagtgcag gaagttatgc
    10501 ttctgaaatg aataattcag taatatttgg ttgtgaattt cctgatggta gctttggcaa
    10561 tgtccatagt gcgaatgaat ttgctagctt agatagattt ataactgcaa taggtatata
    10621 tgcagaggca ataattacat tatgtaataa aatataaata caaaatgtcc cttatataga
    10681 catattatat atacaattaa aattttactt gatatataat tgaaatcaaa gagaaggtga
    10741 tacaatgata actaagaaac atgcaataat agtaaagagt ttaagcaata aagatggata
    10801 tatgacatca aacgaattag ctatcaaatt ggatgtgtct actaaaacta ttaaaagata
    10861 tatagcagat ttaaatagtg ttttaagcaa atatgatttg gtaatagctt cttctagagg
    10921 tataggatac aagttaagcg gttctaaaaa caatatagcc agagctgtta aagaagcaaa
    10981 taaatatatt gatggttttt tagatgactc tgaagagtca agaatgtcaa atataatatg
    11041 tatgcttatt aatagaaatt atatgagtat agaagcaatg gcagaagaat taaatctaag
    11101 tatagccgct ataaataaat tgtctagtaa gcttaaaaga aaacttgaaa agtatgactt
    11161 agttataaaa tctaaacctt actatggaag ctatatacat ggtgaagaaa ttaatataag
    11221 gcaattaatt actgactatg caataaaatt agatgaaaaa agtaaagtga aggtgttttt
    11281 agatgacatt agtgaaaacg atatatactg tatagaaagt attttagaaa agcacttaag
    11341 agaaaaagat acaataattt cagataaaga ttttaatctt ttgctttcaa aaataattgt
    11401 ttctgtattt aggagtaaga gaggtcatag taacaatata aacttaatgg atacaagtta
    11461 tagatttcat aactactact ttatagaaaa tctaatgaaa gaaatatctg ataaaattgg
    11521 atttaaactt attgaagatg aagtaattta tatttcaaat tattctggtg taatagctta
    11581 taaaggtaca caaggaagaa aaaacacaag tgaaattgag gaacgaataa gtagtgttat
    11641 aagtagtgca cttcaagata tattccttat ttcaggtagt gattatacaa aagatgatga
    11701 gtttatggtt gcaatctctg accatataaa gagattttta aatagagcta gagctaatgt
    11761 aaaatcaaat aatcctttac ttcatcaaat aaaagagaag tttcctatag cttttaattt
    11821 agccgtgttt ttatcaaata aactagaaac tgagtttaat ttaaaacttg atgaagatga
    11881 gcttggatat atagctatcc actttgctgc ttcaaatgaa agaatgaaaa aaaatactag
    11941 taagaaaatt tgtatagttt gtcattatgg gataggaaca ggacaactta tttctgaaaa
    12001 gctaaaacag aatataagtg atttaagtgt tgtaggtgtt tatccagtta gatatcttga
    12061 tatggctata agtcaagatg ttgatttgat agtatctacc gttgaattaa aaggatatga
    12121 aaaacctgtc ttatatatag aaaatatatt tgatgatagc ttaattgaaa acgtaaacaa
    12181 agccttttat gaaaaggaag aaagaagaaa gataataagt aacatgtttg atgaaaaagc
    12241 ttttttcagt ataaaggcta gtactaaaga agaggtcata atttcacttt caaataaact
    12301 aaaagaaaga gattttatag aggaaagttc tataaaaagt ataatagata gagaaaatat
    12361 ttcatcaact gaaataggaa atctagtagc aatacctcac acaatagtga agggagataa
    12421 aaaatctata ataggagttg gaatacttga aaatccaata atttgggata agcaagaggt
    12481 tcagctagta tttatggtat tttttaatac taaagaaaag cataatttct cgatatttaa
    12541 atacctttat aattttataa aagatgaagg aggagtaaga ggaattatta aaatatgtga
    12601 ctttaataaa ttaatggcac tgataggtaa ttaaggagga aattaataat gggaacaaac
    12661 atatttaaca aagaatatgt atttttaaat gttgatgcta agtcaaaggt tgaagtttta
    12721 aaatttatat caaaaaaagc taaagactta aatttggcag aagatgagag cttagtatat
    12781 gaaggtttaa tggctagaga agaacaattt actacaaatt taggagaatc tatagcaata
    12841 cctcacacta aaaatgatgc aattgaaaat ccagcagtag tagtattgaa gtttaatgaa
    12901 gatgttgttt ggaatgaagg tgaggataag gttaaattag caataagctt acttatgcca
    12961 ggaaaaagta aagagaatat tcatcttaaa ttattatcat ctctttcaag aaaattaata
    13021 aataaagaat ttaaagattc tcttttaaaa agtgataatg ttgaagaaat atctaactta
    13081 ataaatgaag ctttaggttt ataattaaat actaaggtta taaaataaaa aaataattaa
    13141 ctaaaaatag gaggaatttg aaatgaataa aaaattagta gcattatgtg cttgcccaat
    13201 gggactagct cacactttca tggcagcaga agcaatagag caagcagcaa aggcattagg
    13261 ttatgaagca aaagttgaaa ctcaaggtgc tgatggtgta caaaatgagt taacaagaga
    13321 tgatatatta ggagcaacta tgataataca tgcagtagca ataacaccag aaggtatgga
    13381 aagatttgat ggttgtgaag tttatgaagt tgaattacaa gaagctataa aaaatgcaga
    13441 aggtgttata aaagaaatag aagaagattt aggaatataa taaatttatt gtggaggaaa
    13501 tagaatggca attaagaaaa aagttataaa cagttcaggt gcttctacaa caggaggaaa
    13561 taatgtagga aaatctactc catctagtcc aactcaatca aagggaaatg gaaaatggaa
    13621 agaaataagt aaacatataa tgacaggtat atcatatatg ataccagttt tagtaatggg
    13681 tggacttata ggggccttat ctcaattaat accatatgct atattaggac ttgacccatc
    13741 tgtaggtata gttgatgcta tgaactcagg agaatttaca gggttcaagc ttagtctttt
    13801 aaatatagca caattaatgt caaatttcgg atttactttg tttggatttg cgataccact
    13861 gtttgcagca ttttgtgcaa actctatagg tggaaaaaca gctttaattg caggatttat
    13921 aggtggatat atagctaata aacctgtagg tgtaccacaa ttcattgatg gacaatggac
    13981 tgaagttgtt ccagttgcat caggattttt aggtgcaata ctaatagcat tcataatagg
    14041 atattttgta aaatggttaa ataaatctat aaaagtttct cataactggt tagcttttaa
    14101 aacaacattt ttaataccat taatagcgtc attagcttgt atggtattga tgatatttat
    14161 aataactcca tttggtggat taattaatga aagtatgaaa aacttcttaa cagcagcagg
    14221 agcagctgga gaatatgtat atgcaacagc tttggcagca gctacagcat tcgacttagg
    14281 aggaccaata aataaagcag caggattcgt tgcacttggt ttaactacag agaatgtact
    14341 tccaataaca gcaagaacaa tagctatagt tgttcctcca attggattag gattaacaac
    14401 attattagat aaaagattag taggaagaag agtatatgat agacaattct atcaagcagg
    14461 aaaaacttct atattcttat catttatggg tatatctgaa ggagcaatac catttgcact
    14521 tgagagacca ggttttgtta tacctttaaa catagttgga agtgtaatag gagctattac
    14581 aggtataatt ctaggagcaa ttcaatggtt cccagagtca gctatatggg catggcctct
    14641 agtagataac ttatttggat atataatagg tatagcagtt ggtgctatat tcatagcagt
    14701 aggaaatata ttctatagaa ataaattaat aaaagatggt aaacttgttg tagattatat
    14761 cgactagtat ttaattttta ctatctataa ataactttgg ctggtaatat ggttttaatc
    14821 aatattacca gccttaccta tatgattaat taaaggcggt ggagataaga tatggataag
    14881 atagaaaaag taagagagta ttttagagaa tatgatatag atggatttct tataaatagt
    14941 cctacaaaca agttttatgt tggaaattta tttagctctt caggttatgt ttttataacg
    15001 aaagaatcac aatatataat agtagatttt agatattttg aagaaattaa aagaaaatct
    15061 agtttgttta atgtagtatt aatggacaaa actagaactc attttgatat aattaatgat
    15121 atttgtagag agcagaatat aaaggaaatt ggatttgagg gaaatgaagt atcttttgat
    15181 ttatatagaa gtatgagtaa taagttaagt gcaaccttaa aatcagttga tttatctaca
    15241 ttaagggaaa caaaaaatga agacgaaata aaatatataa aaaaagcttg tgaaatagtt
    15301 gatgctacat tttatcatat agtagacttt ataaagattg gtatgactga aaagcaagtt
    15361 gaaaatgaaa ttgttagaat tataaaagat ttaggtggtc aaaaagaatc tttcgataca
    15421 atagttgcat ctgggcttag aggagcatta cctcatggaa aagcaagtga aaaaattata
    15481 gagtatggag attttgttac ttttgacttt ggtgctaagt ataataatta ttgttcagat
    15541 attaccagaa ccatttgtat gggtactata aataaagagt tagaagaaat atataatata
    15601 gtaagaaaag ctaatgaaga gtgtataaga gttttaagac cgggaatgac tactggagag
    15661 atagataaag ttgcaagaga cataattggt tcatatggat atgccaataa ttttggtcat
    15721 aatctgggtc atggtgtagg aattatggtt catgaatatc ctgccttagc tcctgaatca
    15781 aatgaagtat taaaagaagg aatgattgta actatagagc ctggtatata tgttccaagt
    15841 cttggaggcg taagaataga agatgatgta ctcataacac aagatggatg tatgagattg
    15901 actacatcta ctaaggattt aatagttgta agctaaaaat aaattagata aatattttat
    15961 aacaataaga aaagaagagg taaatttaaa tgtataattt tgatgataaa ccagatagag
    16021 taagtgaaaa gtgtagaaaa tgggatttaa atattataag agataaattt ggagatatta
    16081 gagaagattt tgtaccaatg tggattgctg atatggattt taaaatacca acagaaatag
    16141 aaaacaaatt tattgaagct gtaagaagag gtgtatttgg atatacatac tgttatgatg
    16201 aattttatga ttcagttatt ggttggcaaa aaaatatgca tgaggtaaat gtagaaaaag
    16261 aatggattac attaacttat ggaactgtat caacacttca ttatgtagta caagcttttt
    16321 gtaagaaagg tgatagtata atattaaaca caccagttta tgaccccttt gaatcatcag
    16381 caaaaaaaca aggtgtaaat gtaatttgta atacattaga tgttgtaaat gatagatatt
    16441 atataaattt tgataaatta gaagttcaaa ttaaagaaaa taagcctaaa ttaatgatgt
    16501 tttgtacacc acataatcca tctggaagga tatggacaat tgaagaaatg acaagggtag
    16561 caactatatg taaagaaaat aatgttattt tggttgcaga tgaagtacat gcagagcata
    16621 ttcattatgg taaatttaat tcaatactta agattggaaa agagctttta gaaaatgtta
    16681 ttctactgac atcaccaaat aagggcttta atcttggtgg attaaagact tcttattcaa
    16741 tagttataaa taagaatata agggataaat ttagaaataa attaaagcaa aattctataa
    16801 cttctccaaa tgtgtttgga ataataggat tgataaccgc ttataatgaa tgtcatgagt
    16861 ggttaagtgg tgtaaatgaa tatataaaat caaattatga acttttagaa acttgggtaa
    16921 ataaatataa taagataaaa ttgatgaaaa tggaatcatc ttacttagct tggatggata
    16981 taagtggatt aggtataagt gcaagtgagt ttacagataa attggctata gatacaggag
    17041 tattattaga agatgggagt cattttgtaa aagatggtga gaagtatgtt agaataaatt
    17101 taggaactca aaaggaaaat gtaattgaag ctttaaatag aatggattta tttttaaagt
    17161 ctttataaaa atttggttga attagtattt tatataaatt taagctatta atatggtata
    17221 tacataagta gaatttgttt tagattactt aagtatatac catattttat ataaaaatta
    17281 aatttgcatt agtcatgtga taactattta aattcatatg tattttgtat gtataataga
    17341 tataattatt at
//
LOCUS       DAEPLF010000065        17213 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEPLF010000065 DAEPLF010000000
VERSION     DAEPLF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.065"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(387..779)
                     /locus_tag="KR314_001832"
     CDS             complement(387..779)
                     /locus_tag="KR314_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0328218.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            1347..1709
                     /locus_tag="KR314_001833"
     CDS             1347..1709
                     /locus_tag="KR314_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328219.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1693..2409
                     /locus_tag="KR314_001834"
     CDS             1693..2409
                     /locus_tag="KR314_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328220.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVVEFTEEDEEYEEELKQNIIE
                     NIKKNFDEKKLVQVYKYRGV"
     gene            2429..2848
                     /locus_tag="KR314_001835"
     CDS             2429..2848
                     /locus_tag="KR314_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328221.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2838..3362
                     /locus_tag="KR314_001836"
     CDS             2838..3362
                     /locus_tag="KR314_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328222.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYVDENNNEESISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVLITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            3674..4231
                     /gene="efp"
                     /locus_tag="KR314_001837"
     CDS             3674..4231
                     /gene="efp"
                     /locus_tag="KR314_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBH0328223.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            4323..4778
                     /locus_tag="KR314_001838"
     CDS             4323..4778
                     /locus_tag="KR314_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0328224.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCPSCDDRE"
     gene            4941..5651
                     /locus_tag="KR314_001839"
     CDS             4941..5651
                     /locus_tag="KR314_001839"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBH0328225.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5705..6775
                     /locus_tag="KR314_001840"
     CDS             5705..6775
                     /locus_tag="KR314_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0328226.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6803..10357
                     /gene="smc"
                     /locus_tag="KR314_001841"
     CDS             6803..10357
                     /gene="smc"
                     /locus_tag="KR314_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBH0328227.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKILEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKVSLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEAYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSIATEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIGSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            10384..11664
                     /gene="ftsY"
                     /locus_tag="KR314_001842"
     CDS             10384..11664
                     /gene="ftsY"
                     /locus_tag="KR314_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBH0328228.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDSLENLEETIFSG
                     LEEEVIDKVEDIEEKTEESVNNEGTEEIENFEKIEVEMDSNAKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            11767..12135
                     /locus_tag="KR314_001843"
     CDS             11767..12135
                     /locus_tag="KR314_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBH0328229.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            12132..13484
                     /gene="ffh"
                     /locus_tag="KR314_001844"
     CDS             12132..13484
                     /gene="ffh"
                     /locus_tag="KR314_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBH0328230.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMAELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            13522..13794
                     /gene="rpsP"
                     /locus_tag="KR314_001845"
     CDS             13522..13794
                     /gene="rpsP"
                     /locus_tag="KR314_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBH0328231.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            13859..14086
                     /locus_tag="KR314_001846"
     CDS             13859..14086
                     /locus_tag="KR314_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBH0328232.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            14194..14709
                     /gene="rimM"
                     /locus_tag="KR314_001847"
     CDS             14194..14709
                     /gene="rimM"
                     /locus_tag="KR314_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBH0328233.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            14718..15413
                     /gene="trmD"
                     /locus_tag="KR314_001848"
     CDS             14718..15413
                     /gene="trmD"
                     /locus_tag="KR314_001848"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBH0328234.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVYIYNIRDFSNN
                     KHKKVDDYPFGGGVGMVMTPQPIYDTYKHIITTHNISNPSVIYLTPKGKVYNQSIAKQ
                     MSLKEDIILLCGHYEGVDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            15598..15948
                     /gene="rplS"
                     /locus_tag="KR314_001849"
     CDS             15598..15948
                     /gene="rplS"
                     /locus_tag="KR314_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBH0328235.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            16269..17165
                     /gene="ylqF"
                     /locus_tag="KR314_001850"
     CDS             16269..17165
                     /gene="ylqF"
                     /locus_tag="KR314_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBH0328236.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINNIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIAATV
                     LNEFRDGKIGNITLEVPENVKR"
BASE COUNT         7415 a         1543 c         3187 g         5068 t
ORIGIN      
        1 aaaggttatg ggtaaaatat ttatagaaaa aagtatatta ttgaatagat tataaaattt
       61 tattattttt catcagtctt aaatacacat taatatctat aaaatgacaa aataaaaaat
      121 actatttaaa tagagattaa aaaattgatg cgaagtattt gtttgctata tgaataacaa
      181 tttatttaga agttttgatt tgacactgta taataataaa gtaattgcta aaaaagtttg
      241 atgatttgga tgtattttta gaaattagaa aaatatatac ttactgacta atgataatct
      301 tatatatatt ttagtttgag tgatttaaga aaagtgtttt gaaaggggat tattttatag
      361 aataaatata tatatatagt agaaaattac atataaagag tagtatttat ttcattacct
      421 tctttatctg ttccgatggg gtcttgtaga gatacttgag ttttattttt tttgttagat
      481 ctaatattca ttaaaatctc attttcaata catctagaag catatgttgc taatcgagtt
      541 cccttatcta tgttataagt ttctatagct ttaattaatc ctattgtacc tatggatatt
      601 aaatcatcct gatcttccgt tgaattatta tatttctttg ctatataagc tactaatctc
      661 atgtttcttt caattaatgt atcttttgca cttttatcgt tttctatttt aaatttagtt
      721 aaatactcaa tctcttcttc aggggttaag ggtttttcaa aagatttaag agctgccaat
      781 aggtcacccc cttttcaacg gtcactttaa attatatgaa ggagacttat ccaagttgac
      841 tgttatctaa taaaataata tgcatatttt tagaatttaa aacatatatt tgcaaacttt
      901 tgaatggaat atgacataaa gagtgggaat ttaaatttaa gtttttgttg tatatatatg
      961 aaacaaggcg aagaaattat tgatttataa agtataaatt ggaattgagg tgtcaataaa
     1021 tataaaagga aatagtattt atatatgttt tggtgttgag gtaaaagatt aatgcaaagc
     1081 attagagtct ataaaaatat gaatatagga ggtgtaattt tacaatactt aaaaaagtaa
     1141 atgtaattaa ttgtttgtat aaagttgatt acaagcaaaa agtatcagtc atagttaatt
     1201 ctgttaagga tgaaggtggt gtgttaattt atatatgtat gggaagaaat tttgattaca
     1261 atgaaaaaac ttacaaaaga tatttatgaa aaaacttata gttaaatgtt tatatataaa
     1321 atattattta ggggttgttt ttattattga aaataagaaa gagtggtttt atatcaatcg
     1381 aatgtataat aagtattgct atattatatg tggctgttta tttagtttct acatcattgt
     1441 ataattgtta tagttttatc agtagaaata tatctgacag agaaatgtta agtacagcaa
     1501 aaaaatatat agaagatgag aagtatagaa tacaaaatag taagtatgag ttaattgaag
     1561 ataagataga aaaaaattac ataaatggat atgaaattaa cagtagaata gagcaaattt
     1621 tagattatta tcaatgctat gaaataaata tagagataaa aaatgaattt aaaaaactga
     1681 ggtttaatag ctatgttact agaaaataaa ggctctatat taataataac attgataata
     1741 ttttctatta taagtactgt gtgcattatg tgtataggtt taatttatac acataataat
     1801 atgtttgagt taggatacaa ggacattcaa ctaaaagaaa tttctttaag tggaatagaa
     1861 atatcagtaa gtaatgtact ttcttgtgtt gaggaagcta ttgatgtatc taataatgaa
     1921 gacgaattta gagaatattt cttaggaaat aatatgattg aatgtataaa aaaaataaaa
     1981 gatacatctt atagtagttt agaagaactt tctttaaaag tagaaaataa tactatttat
     2041 gacagaggag atttttttga atttgatata gaaagtaatg ttagagaaaa agaattttta
     2101 aaagcaataa aagtaagagt gaaaatcaaa aatccatgga taggaattga cattgaagat
     2161 tatatggata aatctaataa caataaaatt gatgacaaaa cattcaataa ttataaagca
     2221 tctgaaaata atgaaattct aaatcaattt aaaaatgaaa gtgaaaatat aaggaatcaa
     2281 gaggttgttg aatttacaga agaagatgaa gaatatgaag aagaacttaa acaaaacata
     2341 attgaaaata taaagaagaa ttttgatgaa aaaaaactag tacaggtata taagtatagg
     2401 ggtgtataac tttgaaaaaa gaaggaatgt gactgtaata gaattaatag tatctatcag
     2461 tataatactc ttgatagtta cattatcttt tccaaaagat aatcttgaaa atcataaaat
     2521 aaatttattt gcacgtcaac tttgttcaga tataaggtat gttaggcgaa ttaatatgtt
     2581 tagcaactac aatacatata tatattatat aaatcaaggt aaacacgaag gttatgtatt
     2641 gagaaaagat ggtaaaaaca ttaaatcaat aatgctacct aaaaatgcaa aaatagctag
     2701 aagtattgaa ataataaagt ttaaatctga tgggtcgcct caaaaggcgt ctacaataga
     2761 gatatataat aatagattaa gaaaaactat aacaataaca cctgtaagtg gaagagtact
     2821 tctgaaagag ggaaaatatg aaacataaat atggttactt gcttttggaa agtgtagtat
     2881 cattgtcttc aatggtgata attatactag ttttatattc tatatttttg tctacaataa
     2941 acttaaagtt aaaagtagaa gataagatag aactacaaca acaaagtctt gaaatcataa
     3001 aatctatgga aggaattatt agcaattcta tgggaataat gaatgtatct aattatgaag
     3061 aaacatttaa aaagacaacg tctataaaat gtagatatgt agatgaaaat aataatgaag
     3121 aaagcataag taataaagaa attatattga atgaaagacg aaataagtta tttgtaaatt
     3181 ctttaaatgg cgaaagtagt caagcaggtg gttatgaaat tggagattat gtagatgaga
     3241 tgtatgtatt aattactaat aatggtcaat atgttaatat taaattgaaa ttatcaaaac
     3301 gtagtcaaaa atatgagact gatttcaaaa taaaagtatg gaattttagt gaaagtatat
     3361 aaaaatacat aaatttaaga taaagaaatg acatcaataa gtcatataaa atataattat
     3421 tgagaactat ataactaaat aatatcaata tgtaaagtgt atagattaag aatatagagg
     3481 ataataataa aaatatagaa taagtaattt gccaaatact tgtttatatt gtataactta
     3541 tgatattatg atattagtaa aaatttgtaa tcctgacttg acagaattca agtaagagat
     3601 ataattttaa aggcatagta atgtaaaatt tactaaaaaa taagaaattt attttaatat
     3661 ggaggaacaa aaaatggtat cagcaggtga ttttagaaaa ggtgttacat ttgaaaaaga
     3721 tggacaacca tgtttagtag ttgattttca acacgttaag ccaggtaaag gagctgcttt
     3781 cgttagaact aaatacaaaa acttaaaaac aggagctata agagaagaaa gtttcaatcc
     3841 aagtgaaaaa ttccctaagg ctgttataga tacaagacaa atgcaatatc tatacaatga
     3901 tggtgagtta tattatttta tggatcaaga aaattttgaa caaataccat taaactatga
     3961 gcaagttgaa gatgcaatta agttcttaaa agaaaatgaa gttgctacaa taagatttta
     4021 ccaaggacaa ccattccaag tggaagcgcc aaactttgca gagctagaag ttacagatac
     4081 agagccaggt ataaaaggtg atacagcaag taatgtaact aaagcagcta cagttgaaac
     4141 tggagcagtt gttcaagttc cattatttat aaatactgga gataaagtaa agatagatac
     4201 tagaacaggt gaatatttat caagagtata aaaatttatg tatataaaag cttatatgtc
     4261 ttaataatat aagatatatg agcttttagt ttatatataa aaaagaaagc gaggggtgtt
     4321 acatgaaaca tttatatgaa gaagttgcat acttaaaagg cctagcagaa ggactagaaa
     4381 taagtgctga aagtaaagaa gggaaaatga tacataaaat agttgatgca ttagaagtat
     4441 ttgcagatgc tattgtaaca ttggatgaag aacaagaaga acttcaagat tttgttgaat
     4501 ctatagatga agatttagca gatttagaag aagatattgc tgacatggaa gaagatcttt
     4561 atgaagaaga tgacgatgag gatgatgagg acttcagcta tatagaaatg gaatgcccaa
     4621 attgtggaga gttagtagaa atagacgaag atttgttata tgatgatgaa gtagatgttg
     4681 tttgtcctga ttgtaaggct gtgatattat catcagaaga tgattgtgat gatgattgta
     4741 catgtggtgg ttgtccaagt tgtgatgata gagagtaaaa attcataaga attaaaactg
     4801 cctatgatgc ctatgagata ttttgcttca taggtagttt tatttttatg aggaagggta
     4861 ttatgtattt tggttttgca atacttggac aaaaatagta cactagaatt tatagaatag
     4921 gattttggag ggatgtaaat atgaaaataa gtaaaaaatt actagataat atacaaagat
     4981 ttgaaaatgt tataaattat aaatttaaaa ataaagaata tatactagaa gctcttactc
     5041 atagttcata ttctaatgaa aataaaaaat ataattttaa cgaaaggttg gaatttttag
     5101 gggattcggt tttaggcata gtgataagtg attatttatt taatgaagaa gctaatctgc
     5161 ccgaagggga actaactaaa cttagagcaa atatagtttg tgaagattcg ttaagtgaag
     5221 tagctaatga tataaattta ggtattcata tgttattagg tagaggagaa gaagcaacag
     5281 gaggaagaca taggacttca atattagcag acgcatttga agctgtaata gctgctatat
     5341 atcttgatgg aggttttgaa agtgcaagac agtttatact tcatcatatg gaaaatataa
     5401 tttatgattc aagaaaagga aatatattta gagactacaa aacgcatctt caagaagtgt
     5461 tacaagggaa tggagaaaat aatatctggt acaggcttat agaagaaaaa ggaccagacc
     5521 ataataaaag atttgtaatg gaagttggaa ttaatgacga tgtattgggt ataggagaag
     5581 gaaagagtaa aaaagaagct gaacagttag ctgctaaaat agctttaaag aaaaaattat
     5641 gggaaaaata aatataatat gtaaaaatat aaaaagaaat atttatatta gaaggatgtt
     5701 gtagatgaaa aagagaatta tacctatatt tgttcctcac aaaggatgtc ctcatgattg
     5761 catattttgt aatcaaaaaa aaataacagg tgtgagtact gatgttacta gtgaagatgc
     5821 aagaaatata attgaggaat gtttagagac tatagataaa gatgcagatg tagaaatagc
     5881 attttttggt ggtagcttta ctgctataga tattgatata caaaaaagtt tattatcagt
     5941 tgctaaagaa tatgtagaaa aaggtcttgt aaaagatatt agaatgtcta caagaccaga
     6001 ttgtattgat gaagatatat taagtatgct aaaagaatat aagacaagta taattgaact
     6061 aggagtacaa tccttagatg aaaaagtatt attagatagt ataagaggtc atcaatctga
     6121 aattgtatac aagagttcaa agatgataaa aaatagtgga atcaaacttg gattacagat
     6181 gatgattggt cttccagctg acacagaaga aaagtgtata tttacagcaa aaaaatttgt
     6241 agagctaaaa ccagattgtg ttagagtcta tccaactttg gtagtaaaag atacaggtct
     6301 tgaaaagtta atggaacaaa ataaatataa tccatttact cttgaagaga gtgtaaatat
     6361 agtaaaaaaa gttcttgtac tgttttatgt taataatatc aatgtaataa gagttgggct
     6421 tcaagctact gatgatatac aaattggaaa agctgtttta gcaggaccat atcatcctgc
     6481 atttagagag ttggtagaag cagatatgat aaaagattat ttagaatttg ttattttgca
     6541 aaataaaaat ataaagcaaa tgttggtcaa atctaataaa aagaatattt ctaaaatcat
     6601 aggaaataaa aaaacaaatg ttaaatatat ggaagaaaaa tttggtgtgc ttttaaaaac
     6661 acaagaatcc gatttagata taaatcaatt agaaattgtg ttagatggga aaagtttaat
     6721 tagtgctaat atgagagaca ttcatagaaa attgtatgac atttatgacc tttagtttta
     6781 ataatgaagg gagagaaaag atttgtattt aaaaagacta gagttaaagg gatttaaatc
     6841 ctttccagta aaaacagata ttatttttaa agaaggaata acagccatag taggaccaaa
     6901 tgggagtgga aaaagtaata tatctgatgc tgtaagatgg gtattaggag aacagagtat
     6961 taagagtctt agaggtgata aactcgaaga tgtaatattt gcaggtacag atacaaaaaa
     7021 acctatgaat tattgtgaag tggctcttac tattgataat tctgaaaatc aattggaatt
     7081 agattttaca gaagtaacta taagaagaag ggcttataga aatggtgaaa gtgaattttt
     7141 tctaaataat aaaagttgta gattaaaaga tataaaagaa gtctttttag atactggtat
     7201 tggaaaagat ggttattcaa tcatagaaca aggaaaagta gatgaaatat taagtaataa
     7261 tcctttaagc agaagaaaag tttttgatga agcttgtggc atatcaaaat atagatataa
     7321 aaaacaggaa gcagaaagga atttaagtaa tacaaaggaa aatttagaaa gaatagatga
     7381 tgtatatata gaaattgaaa atcaattaaa gcctcttttt aatcaacaga caaaggcaaa
     7441 aaagtacttg gaaataagtg aaaaattaaa gatacttgaa gtaaatagct ttataagaga
     7501 aattgaagga atagaaaaag aattaagtga ggttaatgaa catcgtaagg ttattgaaaa
     7561 ggagctcaat gaaaaagaag aacaaaaaaa tgttgtagag aaaaagcaag aagatattaa
     7621 taaagaggta gaggtattac aagatgtaat tgaaaaatct gtagattata taaattcaat
     7681 taaaggagtt atatcaaaaa aagagtccca aataaattta attaaggaaa gaataagaaa
     7741 ttttacgaat gaaattagta gaaaaaattt agaaataaat gatataaaag agaagctaaa
     7801 tgaaaataag caatacataa aagaattaga aagtaataaa ttatcaggta gtgaagaact
     7861 atctaccttg caggaaaata taaaagtatt ggaggcaagt aaagataaac aaaaaattaa
     7921 gttagaatcc ctaaataatg agatagaatt attgaaagaa agtataattg atatattaaa
     7981 taagaaacaa gagtttagta ataagctgtc cactttaaac gcaaataaag aaaatatgaa
     8041 tatcagagat gaaaatataa attcagagat aacagagtta aacaaaaata ttgaaataaa
     8101 atcatctgaa ttagatacta taaataaaga atttaatatg caaaatgaaa atttaaagaa
     8161 tgtaaataat agacataaag aattatcaat taatttgcag gattcaatta gtgaacacaa
     8221 taaactagaa gatgaaatac agaaaagtaa atataattta aatggatata attcaaagtt
     8281 aaatgtctat atagatatgg aaaatcatta tgaaggattt aatagaggag taaaagaagt
     8341 actaaaaaat aaaaatttaa aaggagttca tggagcatta ggacaaatta taaatgtacc
     8401 tgaaaaatat gagaaatcta tagaagcagc tcttggtgct tatatgcaaa atataataac
     8461 tgataatgag tttagtgcta agtctgcaat aaactatcta aaacagaata atttaggtag
     8521 agtaacattt cttccattaa atataattaa atcaaataaa gtaagtctag gaaatttaaa
     8581 agcaaatact aagttcatag gaatagcaag tgatttaatt acatttgatg aaaagtatag
     8641 aaatataatt gaaaatatac ttggaagaac aatacttata aataatatag atgagggcat
     8701 aaaatttgca aaagaaactg gtcataggtt taaaatagta actttagatg gtgaaatctt
     8761 aaatccagga ggttctttga ctgggggaag tctaaaaact aatgggaata tactatcgag
     8821 aaaaagatac attaatgaat atacagaaaa gataagtaat atcaagaatg aaatttcaca
     8881 tctagaatta aaaagagaat ctttagacaa agatgtgaaa aatatcaaaa atgaaattga
     8941 ttcacatgaa agtaaaataa aagatttaga aaaaagtata ataataaaaa gtacaagtat
     9001 aaagaatgtt gaatctgaaa ttgaatcgtt aaaaggtagt attactaaat tagaaaatga
     9061 gaagaatgat ttaaattcta atctaaatta tacacttgaa aaaagcgatg atgttagaaa
     9121 agatatggaa gagttagatg atttatacaa taaaaataaa gaaaaaattg atgcattgaa
     9181 tgaggaaata aaaagataca atgatttata tgataaagaa aaatctgaat ttgatgaatt
     9241 gaacttaagt ttagtgaaga aaacagaagc ttataatagt atagttagag atataaaaag
     9301 aatatctggt gaaaattgtg agctagaaga aaagaataaa caattagaag agtcattaaa
     9361 ttatgaagaa catgaaataa ttaaattaca agattctata gccactgaag aaaaagaaaa
     9421 agaaaattta acaaaacaac taggggatag taatagaaat cttgaaacta gaaaaatagc
     9481 aaaagatgat ttaaaaaata gttttgatga aattaataaa gaattaaaaa ctatagatag
     9541 acagcatata gagcttaaag aaagtttatt taaagttggt ggaagattag aaagattaaa
     9601 aactagtcaa gacacatata taaataagtt gtttgagcaa tatgatatga ctttagtaca
     9661 agcattggaa attaaagatg aagatttgga tatagataga aaattcttag aaagtctaaa
     9721 gagagaaata agaagtcttg gaaatataaa tataggctct ataaaggaat atgaggaaat
     9781 aaaagaaaga tacgattttt atagcgaaca aaagcaagat ttagaagagt ctatggaaga
     9841 gatagaaaaa cttatacaca ctttagaaga aaatatgaaa tctgaatttg aaattaagtt
     9901 tgaagaaata agtaaaaatt ttaaatatgt atataagaga ttatttggtg gaggttgtgg
     9961 agaattaact attttagata aagaaaattt attagaaagt gatatactaa taacagctca
    10021 acctccaggg aaaaaaatga aaaatttaaa tctattatct ggtggagaga aagctttaac
    10081 agcaataagt atattatttg ctatattgat tacaaagcca acaccatttt gtatattaga
    10141 tgagattgaa gcgccacttg atgatgcaaa tatatttaga tttggagaat ttttgaagga
    10201 tttatctaaa gagactcaat ttatatcagt aactcatagg agaggaacta tggaagctgc
    10261 tgattatatc tatggagtta caatgcaaga aaaagccatt tcaaaagtta ttagcttaaa
    10321 attaaaagaa gctcaggaaa taacagatat tatataactt agattgacat aggaggaata
    10381 acagtgttaa aaaaattatt tggttttgga aaagacaagg aaaaagaaat agaaaaaaaa
    10441 gatgctgaag aagaaataga agttgaagac agtgttgata gtttagaaaa cttggaagaa
    10501 accatttttt caggacttga agaagaagtg atagataaag tggaagatat tgaagaaaaa
    10561 actgaggaaa gtgtcaataa tgaaggcact gaagaaatcg aaaattttga aaaaatagaa
    10621 gttgagatgg atagtaatgc aaaagaagta gaaaatattt caaataatga ttctcaagaa
    10681 gagataatcg aagaattaga aaattatgat gaaattgagt ctaaagagat agaagataaa
    10741 gaagataaga aagttaactt gtttgaaaga ctaaaacaag gattgacaaa agcaaagcaa
    10801 ggaataactg atagaataga tgaagtttta aaatcatata caaaaataga tgaagaatta
    10861 cttgaagatt tagaggaaat tttaataaca gcagatgttg gtgtaaatac aactatggat
    10921 ataattgaaa gactaagaga taaaataaag caaaaaggta ttactgaacc aataaaggta
    10981 agagaagaac ttaaatctat agttgaagat attcttacaa atgaaaactc tacactagat
    11041 atagaaccag caccatgtat aatattaatg gtgggtgtaa atggggtcgg taagactact
    11101 acaataggta aactagcaaa tagatacaaa aaagatggta aaaaagtttt attagcagca
    11161 gcagatacat ttagagcagc agcaacagag caattagaaa tttgggcaaa tagaaccaat
    11221 gtagacataa taaaacatca agagggagca gaccctggag ctgtagtatt tgatgctata
    11281 aaagctgcaa aagctagaaa aacagatgta ttaatatgtg atacagcagg aagattacac
    11341 aataaggcta atcttatgaa tgaacttgga aaagtattta aaatagtaga tagagaattt
    11401 ccagaagcaa aacgtgaagt acttcttgtg gtggatgcta caacaggtca aaatgcagtt
    11461 gttcaagcaa agacatttaa agaagttgca gatataacag gtatagtact tacaaaactt
    11521 gatggaactg caaaaggtgg agtagtcctt gcagtaaaat cagaagtaga tgtacctgta
    11581 aaacttattg gagttggaga aagtgtagaa gacctacaag atttcaatgc aaaatcattt
    11641 tcagatgcat tatttggtaa ctaatgttct tttaaaaata atggttgaca agagtgttgt
    11701 tatgatataa aatacaatag tgtaaagtaa ataaccttta cagtatactg ctaggaagtg
    11761 taagatatga atatagaaaa aatggtcgaa attggattgt tatttgaaca gtataaagaa
    11821 ttattaactg acaagcagaa agaaatagtt gctctatact atgaagaaga ttattcttta
    11881 ggtgaaatta gtgaaaatct gaatgtatca agacaaggtg tttatgatac cttaaagcgt
    11941 tcagaaaaaa tactaagaga ttatgaagaa aagttacact tagtatcaaa gattcaagag
    12001 caggaaaaaa atattaagat tataaaagat aaaattattg acattaaaga agatttattg
    12061 cacaatagag attgtgctaa tttaatccct aagctagaaa atatagaaga tgtatgtagg
    12121 gagatgataa aatgatattt gaaggattgt ctgataaact acaaggggca tttagtaagt
    12181 taaaatctaa aggaaaactt acagaagtag atgtaaaaaa tgctatgaga gaagttaaac
    12241 ttgctcttct tgaggcagat gttaacttta aagtagttaa agatttcata aaaaaagttc
    12301 aagagagatg tgttggacaa gaagttatgc aaagcttaac tccagctcag catgtaatca
    12361 aaattgtaaa tgaagagctt acaagtttaa tgggagatgt acaaagtaaa gtaatgattt
    12421 cctcaaagcc tcctacaatt ttaatgatgg ttggattgca aggtgcaggt aagactacta
    12481 cagcaggaaa acttggagga tattttaaaa agcaaggtaa aaaacctcta ttagtagctt
    12541 gtgatattta tagaccggca gctataaagc aacttcaagt tgttggagaa aaattggata
    12601 tacctgtatt caatatggga gataaagaaa atccagtaaa tattgcgaaa gcaggtttaa
    12661 gccatgcgat taaaaatgct aatgatttag ttattataga tacagctggt agacttcata
    12721 tagatgaagt acttatggct gagttaaagt ctattaaatc agaagttaag ccacatgaga
    12781 tacttttggt tgtagattct atgacaggtc aagatgctgt aaatgttgca gaaagcttca
    12841 atgaggcact tggtgttgat ggagtagtat taactaaatt ggatggagat actagaggtg
    12901 gtgctgcact ttcaataaga gcagttacac aaaagcctat aaaatttatg gggatgggag
    12961 aaaagctaga tgatatagaa cctttccacc cagatagaat ggcatcaaga atattaggta
    13021 tgggagatgt cttaagttta atagaaaaag cacaagaaaa tatagactta gagaaagcta
    13081 aagaattaga gcaaaaaatt aaaaaacaag agcttgattt tgaagatttc ttacaacaaa
    13141 tggaacaaat acaaaatatg ggacctcttg acaagatact tggtatgata cctggtatgg
    13201 gaaatgtaaa agaccaactt ggagatgtag atttgaatgg caaagaaatg aagagaacaa
    13261 aagccataat tcagtcaatg actacatatg agagaagaaa cccaggagta ttaaatgctt
    13321 caagaaagaa aagaatagct aagggcagtg gaactagtgt acaagatgta aatagactta
    13381 taaaacaact taatgaaatg aaaaaaatga tgaagatgtt tgctggttca caaaagagta
    13441 tgaagaaaag gggcggctta ggcggtttac ctttttttaa ataaaatata aaaaagtaaa
    13501 aattattcgg aggtgactgt aatggcagtt aaaataagat taaaaagaat gggagctaac
    13561 aaaaaacctt tctacagaat agtagtagct gattcaagag ctccaagaga tggaaaattt
    13621 atagaagaaa taggatacta taatccaatt tctgaaccta aacaagtaag aataaatgat
    13681 gaaaaagcta taaaatggtt agctactggt gctcagccaa cagaagtagt taaaaaatta
    13741 cttgtaaaaa acggagtaat agaaaaattc gaagcttcta aacaagcaaa ataatttttt
    13801 cttggcaatt agtttaagta agggctcatt gctaaggtta ctttaggaga tgaaatatat
    13861 gaaagagcta gttgtagata tagctaaggc tctagttgat aatcctgact cagttgttgt
    13921 tgaggaattt gaagataatg atggtatcgt cttaaagctt acagttgctc aagaggatat
    13981 gggtaaggtt attgggaagc agggaagaat agctaaagct ataagaactg tcgtaagatc
    14041 tgttgcaaat agagaaaata taaaagtttc tcttgagata gtataaaata ggaggattag
    14101 gtataatgcc tagtcctttt tatataaatg cgtagtttaa gattttatga tgatatgacc
    14161 acataagatt aaatttttta agaaggtgat aacatgaaag aaaaattaac tcattttaag
    14221 gtaggtcaaa tagtaaatac acaaggttta aaaggtgaag taagagttta tccacttact
    14281 gatgatatag agaggtttga tgaattaaaa gatttctatc tgggaaaaga tttaaataat
    14341 aaattggctg ttgaaaaagt aagatacaaa ggttctatgg ttataatgaa gataaaaggc
    14401 atagacagca tagagaaagc agaaaaacta aaaaataaat ttatgtatgt atctagagaa
    14461 gatagcagag agcttgaaga aggcgaattt tttatagcag atatgattgg aatggaagtt
    14521 ttaactgtag atggaaaata tgtaggaata ctagaagatg ttcttcaata ctcagcaaat
    14581 gatgtttatg tagtaaaagg tgaagaagat aaagaattta tgataccagc tataaaaaaa
    14641 tttgtaccta caatagatat tgatgaaaga aaaatgatta tagaccctat taagggaatg
    14701 atagattagg tggtaatatg agatttcata taatgacact ttttccagaa atatttaatt
    14761 cgtacatgga tgaaagtata atgaagagag cagttgaaaa aggtataatt gaggtttata
    14821 tatataatat aagggatttt tctaataata aacataaaaa agtagatgat tatccatttg
    14881 gaggaggagt ggggatggta atgactcctc aacctatata tgacacatat aagcatataa
    14941 taacaactca caatataagt aatccaagtg taatttatct tactccaaaa ggtaaggtat
    15001 acaatcaatc tatagcaaag cagatgtctt taaaagaaga tataatacta ttatgtggcc
    15061 attatgaagg agtagatgag agaatcattg atttaatagt tactgatgaa atttcaattg
    15121 gagattatgt attaactggg ggagagctcc cagcacttat aatgatagat tctatatcaa
    15181 gattaatacc aggtgtatta aaccaagaag aatcatttga agaagaatca tttaaagata
    15241 acttattgga atatcctcat tacactagac ctagagattt tgaaggatta aaagtaccag
    15301 aagtgctttt atcagggaat cataaaaaga tagatgagtg gagaagagaa gaatctataa
    15361 ggataaccaa agaaagacga tttgatttat ataaaaagtc taatgaaaaa taaaaatata
    15421 atgaaaaatt aatatatatg tatacaataa agtacctata aatggaatta aaaacataaa
    15481 agtgcttgta ataactattg aagtatagta taatataaaa tgttgaaaat acgggcgttc
    15541 ctctgtttac actggtcggg tagttaagaa cgtctatgat atctaggagg aagaaagatg
    15601 aatgaaatgc taagagcaat tgagcaagaa caattaaaaa atgaagttcc taattttgga
    15661 cctggggaca cagttaaagt acacgttaga attatagaag gaaaaagaga aagaatacaa
    15721 gtattcgaag gtgtagtatt aaagagacaa ggtggaggag caagagaaac tttcacagtt
    15781 agaaaaatgt ctttcaatgt tggtgtagaa agaactttcc cagttcactc tccaaaaata
    15841 gaaaaaatag aagttactag aaaaggtaaa gtaagaagag ctaaactaaa ctacttaaga
    15901 ggtagagtag gtaaagctgc taagataaaa gaagctagaa ataaataatt aaaaacttta
    15961 cttataaatg gggggctgat ttatcagtcc tctttctatt ttaatgaaaa ttacagcaaa
    16021 taattatact agaaataaat agaaatattt gttgaaaata tacattattt atctatgaat
    16081 ttatagcttt taaaatgaaa aataaaattg gtaaatattg gattatattt tgtaacgata
    16141 ttaaaaataa gagaacagta ttatgtaaag tatgacgtta aaaagatgac aatattatag
    16201 tttgtgaaga tagatttttt aagtggaatt agaatataat aatataatat taataaggag
    16261 tgatacatgt gtcaaatgaa tacgaagagt atcttatgga tgacaatcta catataaact
    16321 ggtatccagg tcatatgaaa aaaactaagg agttagtaaa aaataactta aaattgattg
    16381 atgtagtaat tgaacttttg gatgctagaa tcccgtttag tagtaaaaat ccagacatag
    16441 acaggcttgt aggagataag ccaagagttg tcgtacttaa taagagtgat atggcagata
    16501 gggataaatt gaatcaatgg attgaatact ataagaaaat taatatcaaa gctataccag
    16561 tagatacaat aaaaggtgtt ggtataaata atataatcga agaatgtaaa aatgtaacta
    16621 gagaaaaaat gagttcttta aaagacaaag ggagaaaaga aagagctata cgaataatga
    16681 tagttggagt tccaaacgtt ggtaaatcgt ctcttataaa caaattaaca ggtagaaaaa
    16741 gcactcaaac tggagataag ccaggagtaa cgaaagggaa acaatgggtt agattaaagg
    16801 gaaatcttga acttttagat acacctggaa tactttggcc taagtttgaa gaccaagaag
    16861 ttgcattaaa tttagcattt agtagagcta taaaggatga gattttagat actgagactt
    16921 tggctttaag acttatagaa aaacttatga aaatagaacc tgagaagttg aaagccagat
    16981 ataagttaga ttgtctagga gaaactccaa ttgaaactat ggatatgatt ggccataaaa
    17041 gagggtttat aacaggtaaa aaagaactag attatacacg catagccgca actgtattaa
    17101 atgaatttag agatggaaaa ataggaaata taacattgga agttccagaa aacgttaaaa
    17161 gatagatttt aaaacaaaat caaagattta atgttaaaaa aataaaaaag cct
//
LOCUS       DAEPLF010000066        17182 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEPLF010000066 DAEPLF010000000
VERSION     DAEPLF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17182
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.066"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(91..1548)
                     /locus_tag="KR314_001851"
     CDS             complement(91..1548)
                     /locus_tag="KR314_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBH0328237.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            1966..2880
                     /gene="nadA"
                     /locus_tag="KR314_001852"
     CDS             1966..2880
                     /gene="nadA"
                     /locus_tag="KR314_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBH0328238.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            2893..4194
                     /locus_tag="KR314_001853"
     CDS             2893..4194
                     /locus_tag="KR314_001853"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBH0328239.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            4175..5011
                     /gene="nadC"
                     /locus_tag="KR314_001854"
     CDS             4175..5011
                     /gene="nadC"
                     /locus_tag="KR314_001854"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBH0328240.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            5267..5599
                     /locus_tag="KR314_001855"
     CDS             5267..5599
                     /locus_tag="KR314_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBH0328241.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            5761..5946
                     /locus_tag="KR314_001856"
     CDS             5761..5946
                     /locus_tag="KR314_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328242.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            5973..7058
                     /locus_tag="KR314_001857"
     CDS             5973..7058
                     /locus_tag="KR314_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBH0328243.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLYNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSTIPKMKEVIGNHA"
     gene            7051..7509
                     /locus_tag="KR314_001858"
     CDS             7051..7509
                     /locus_tag="KR314_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328244.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            7544..8635
                     /locus_tag="KR314_001859"
     CDS             7544..8635
                     /locus_tag="KR314_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0328245.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKAGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            8640..8864
                     /locus_tag="KR314_001860"
     CDS             8640..8864
                     /locus_tag="KR314_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328246.1"
                     /translation="MENIKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            8874..9437
                     /locus_tag="KR314_001861"
     CDS             8874..9437
                     /locus_tag="KR314_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328247.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            9398..10027
                     /locus_tag="KR314_001862"
     CDS             9398..10027
                     /locus_tag="KR314_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0328248.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            10067..10204
                     /locus_tag="KR314_001863"
     CDS             10067..10204
                     /locus_tag="KR314_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0328249.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            10204..10956
                     /locus_tag="KR314_001864"
     CDS             10204..10956
                     /locus_tag="KR314_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328250.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            10969..11454
                     /locus_tag="KR314_001865"
     CDS             10969..11454
                     /locus_tag="KR314_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328251.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYENIMVVDGNSINFIYPVSALQTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            11461..11610
                     /locus_tag="KR314_001866"
     CDS             11461..11610
                     /locus_tag="KR314_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328252.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(11746..12567)
                     /gene="yidA"
                     /locus_tag="KR314_001867"
     CDS             complement(11746..12567)
                     /gene="yidA"
                     /locus_tag="KR314_001867"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBH0328253.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNEAPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            13059..14753
                     /locus_tag="KR314_001868"
     CDS             13059..14753
                     /locus_tag="KR314_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBH0328254.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSHFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGNIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKISKKLGFEDFVEL
                     GYVRMMRSDYREDMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      15085..15195
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            15299..15670
                     /locus_tag="KR314_001869"
     CDS             15299..15670
                     /locus_tag="KR314_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBH0328255.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQDNKKTPKWTENVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            15766..16713
                     /gene="trxB"
                     /locus_tag="KR314_001870"
     CDS             15766..16713
                     /gene="trxB"
                     /locus_tag="KR314_001870"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBH0328256.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            16798..17115
                     /locus_tag="KR314_001871"
     CDS             16798..17115
                     /locus_tag="KR314_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBH0328257.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT         6827 a         1860 c         3029 g         5466 t
ORIGIN      
        1 taaataaaaa agaggcaaat ctaacttgag attgcctact tttttatcat tttttaataa
       61 aatttattct aagtattatt ttatttagat ttagtcagcc aatttagtat ccgctttgct
      121 ttttaacttt ctaccagagc gtacagttaa aatagcatat aatacagtaa atatggcagc
      181 taaaacatat gatatattta tgtttaagtt aaatccaatt tgagcattaa atatataact
      241 gaatataaca aaagaataaa acataccagg aattaagctt ataatataat tcttttcttt
      301 tattataagg taaactgtaa tcattgcaaa tgcaaatatg gcaatagttt gattactcca
      361 agagaagtat ctccacaata tattaaatcc agaagcacta agctttgcaa atataagaat
      421 ggctatgaca ggaataaata tacagattga caatataaca cgatgtttct tttccttttg
      481 gtcataatga aagtaatctg caagcattaa tcttaaagat cttaaagctg tatctccaga
      541 agttattggt aaaacaatta cacctattat agctatgata ccaccaatag aaccaagtaa
      601 atctcttgca accaaaccta ttacatcagg agaacctact aattccttag gtattccttt
      661 attgtaaata cccatagcag ctgcagccca aatcattgca attaaacctt ctaaaatcat
      721 catattataa aaagtagttt ttccttcttt ttcatttgaa actgatcttg ctataagagt
      781 agcttgagtt gagtggaatc cagaaacgat accacaagca actgtaacaa agaatgtagg
      841 aataagagga ataccatcag gatgaatacc tttccaattt gcaagactta aattagctaa
      901 atcataacct tgtgtaaaga ttccaattcc aacaccagca gcagataaaa gtagaatagc
      961 tccaaatatt ggatatacct ttcctatgat tttatcaatt ggaaagagtg ttgcacacaa
     1021 gtaatataga aagataagtc cataaactat ccagacaact ggattattta tagtcgattt
     1081 catatttaaa atttgagtaa caactaaatc cccaggtgta taaataaaaa ctgcaccaac
     1141 taaaagcatt aaaagacaaa ggaatatatt atagacttga aatactttat tacctaaaaa
     1201 tctactgata agagaaggca tttgtgctcc tttttctctt atactaatca ttccactcat
     1261 atagtcatgc aaagctcctc caaatacaca gccaataggt attagtatga atgctattgg
     1321 accaaacaat ataccttgga taggtcctag aattggtcct gtaccagcta tattaagcaa
     1381 ctcaattaga ctatttttcc attttttcat tgggacatag tctactccat cactttgagc
     1441 aagtgcaggt gtttttctgt catctgggcc aaatactttt tcacaataag caccgtataa
     1501 agcacctcct attagtaata tagccaagcc gattaagaat gttgtcataa tatcacccca
     1561 tttataaaat tttttggtct ttgtatacat attatctaaa tgtgtagatt tatattgaat
     1621 ttaattctgg acaacctaca aattctataa agtattacaa ctaatataat ttcagaaata
     1681 aatacatata cataaccatg tacttagtat acaggtattt ggtaatattt tcaatgttat
     1741 agcaataaag aatataaaca aaattctgat aatattttac aaaaaaatta aaaaatataa
     1801 aaattagttt aaatagatgg agaaaaaagg aaaatcaatt gagaatataa atgtctatat
     1861 cttaacaata ttaagaggtt taaaattctt gaagaaagaa tagtcaagtg atataatggg
     1921 tttacaggtg tatatacact agtacatata gaaagcggtg caattatgga taaagattta
     1981 acatatcaaa tcaaagagct aaagaaagaa aaaaatgcaa taatacttgc ccatttttat
     2041 caaccaccag aaatacaaga attagcagat gcagttggtg actcatatta tttaagtgag
     2101 attgctagag attgtaaaga agaagtggtt gtattttgtg gagtaaggtt tatgggagaa
     2161 agtgctaaga tattatcacc tgaaaaaact gtattgatgc cagtaagtaa cgctgggtgt
     2221 gctatggcag atatggtaga tgaagaaggc gtgataaaat taaaacaaca gtatccaaat
     2281 gctttggttg tatgttatat aaactctaca gctaaagtta aggcacattg tgatgtttct
     2341 gttacatctt ctagtgcaat taaaatacta gaaaacatag ataataaaga aattatattc
     2401 cttccagata aaaatttagg aggatatatt gcagaacaat ttccagataa aaactttata
     2461 ttttgggacg gatattgtaa ataccataac aatataagag ctgaagaaat aattgaatta
     2521 aaagataagt ataaaaatgc agaagtatta gtacatccag aatgtaaaaa agaaataaga
     2581 gatttaggag attatgtagg tagtacatcc ggaataataa aatatgctac taatagtaaa
     2641 aacaaagact ttataatagc aactgaagaa ggtatattac atgaattaaa gaaaaataat
     2701 cctaataaaa acttttatat tcctggtgga aaaatactat gtacagatat gaaaaagaca
     2761 acattagaaa atttatatag tacacttaaa aatatggaaa atgaagttat tgtagaagat
     2821 gaaatcatgg aaaaggcttt aaattcactg cttaacatgc acaaattagc agaaggatag
     2881 agggatagac ttatgaattt agaacaagat gttttaatag ttggaagtgg agtttcagga
     2941 ttatactgtg cattaaattt agataaaagt ttaaatgtgc tagtagtatc gaaatcaaca
     3001 attgaaaaca acaatacata tcttgcacaa ggtggaattt caactgctag aaatattgat
     3061 gatatagaat cctttgtaga agatacaatg aaggctgggc agtataaaaa tagagttgaa
     3121 gcagttcaag tacttgcaga tgaatctata gaaaatgtag ggcagatagt agaatatggt
     3181 atgcctttag acaaagaaaa tggagagata gattacacaa gagaaggagc acatagtgta
     3241 aatcgaatag ttcatagtaa ggataatact ggagaagttg tttttaaaac tttattaaaa
     3301 gaagctaaaa ctagagaaaa tataactctt atagaagatg catatctttt agatatatta
     3361 aaagatggca ataaatgtat aggagctaga atttttaaat ctaaaaaaga gattcatgtt
     3421 ttttcaaaaa tagtagtttt agctacaggt ggaattggtg ggctttttaa aaattctact
     3481 aatcaaagac atcttacagg tgatggaatt gcaatagctt taagaaataa tataaaaata
     3541 gaaaatttag aatatattca aatacatcct acagcctttt atgaagaaaa taatgaaggt
     3601 aggagaatgc ttatatctga atcattaaga ggagagggag ctaaactttt aaataaaaat
     3661 aaagaaagat ttgtggatga acttcttcct agagatgtag tttcaaaagc tatttttgag
     3721 caaatggaaa aagataaatt accttatgtg tatttagatg caacacattt agatagtgaa
     3781 tatttaatta atagattttc ttttatttat aatgaatgtt tagctagagg aacagatata
     3841 acgaaagaat gtataaaggt atctcctgca caacattatt ttatgggcgg gatacatgta
     3901 gatttggatt caaaaacatc tatggaaaat ttatatgcag ttggtgaaat tagttgtaca
     3961 ggtgttcatg gagccaatag attggcaagt aattcattac ttgaagggct tgtattttca
     4021 aaaagagcag ctaaaaatat aaactcagtt atagataatg taaaagttaa gtttatagat
     4081 gtaccagata tggatataga tatagaacag gtaaaaaaag aaaataaaat attagtcata
     4141 aaagaaattg aaagaacaag tgaggatttt ggcgatgaat tatttgatta ttgatagaat
     4201 gatacaggat gctttgatag aagatgttcc aagtgaagat ataacaacta attctattgt
     4261 ggatgaaaat tctaaatcta ctgtagattt aatttgcaaa caagatggta taatagctgg
     4321 cttaggtgta tttaagagag tttttgagat acttggtgat gtagatgtaa aattatataa
     4381 aaatgatggt gatagagtaa agaatcgtga aaagatagct gtattaacag gtagtactag
     4441 aaacttactt gtaggagagc gtgtagcatt gaattattta cagagaatga gtggtatagc
     4501 tactattaca agtaggtatg ttgaaaagct tgaaggtaca aatacaaaat tattagatag
     4561 tagaaaaacg atacctaatc ttagaatatt agataagtat tctgttaaag taggtggagg
     4621 atgcaatcat agatttaatt tatctgatgg aatattgctt aaagacaacc atattggggc
     4681 tgctggtagt gttaagaagg cagttgaatt agctagaaaa aatacttctt ttgttagaaa
     4741 gatagaagtg gaagtggaaa ctttagacat ggtaaaagaa gcaatagaag ctaatgcaga
     4801 tataataatg ttggataata tgagtttgga aatggcaaaa caagcagtag atattataaa
     4861 tggaagagcc attgtagaat tttccggaaa tgtaaattta gatactatag aagatatagg
     4921 aaaaattggt gtggatgttg tttctgtagg agcgcttaca cattctgtta aagctctcga
     4981 tataagtatg aagaatttga gaaatatata agttttatat tgaaataaac ttaattaaaa
     5041 tagactcctt aaatttgttt tataaattta aggagtctat tttttattca ttaaaattaa
     5101 taaaaatata aaaaaatcaa tcaataattt aataaaaata gataaatact atatacactt
     5161 ttaataatat agctaatatc tattttacag ataaataaga ataaacttat aatataattg
     5221 tatctgtaaa attttgtaaa atataaaaag tataggggga ataaaaatga aagtagctta
     5281 tatttttagt agtactaata cacataaaat tctagacaaa atgataatac cacaacttga
     5341 acaaggaact catggagtag acgttctagg gatgtttttc tttatggata ataccttatt
     5401 tttatctaaa ggtaatccag taggcgagag actaagtaaa ttacatgaaa aaactgatat
     5461 gataataatg gcttgtgacc aatgtgctat agaaagaggt atagagaata atttggttga
     5521 tggagctaca attggatgtt tcccaaacct atatgcttgt ttaggcagtg caggtgtgga
     5581 ccaagttata acgttataaa ttagtatagg atacaatcca tagaaaaatt tattttctgt
     5641 ggattatttt aagtaaaaga taaattgttt aaagtagggg atgaacaaat gtctaaaatt
     5701 ttagttgaat ttataaacac ttgaccatct tgtgatgaac atggagtgtt tataaaaaaa
     5761 atgtcagaaa agtatgctga taaattggaa ataaaattat atcaagcagg gaaagatttt
     5821 tcgtatataa aaaaatatgg gattataaca aaaggaactt taataataaa tcagaaaaag
     5881 aaatatgata gattaaataa agatacaatt gaaagggcaa tagtagaagc tataaacaat
     5941 aattaaaagt ttaaaaatta ggagtatcaa aaatgaatat attagaaatt gttttaaatg
     6001 gagttaaaga tactatagta gcttctatag ttacactgaa tggagcatca ggatggctta
     6061 tgataagttt tatattggca ggtgtacttc acaatgtttt aactccaaga aggtttcaaa
     6121 aacaacttgg aaataagaaa ttttcttctt taattaaaag tactatctca ggaatgtttt
     6181 taccaatatg tagttgtgga gtaataccac ttggaataag tatgtattat tcaggagcat
     6241 atttaggacc agtacttgct tttatgacat caactccaat aataaatcca atagctgtag
     6301 ttctttgtct aggtctttta ggtcctaaag ttacaatcat ttatctaata gctggttttt
     6361 tagtaccatt tttagtagga tttttaggaa attgttttgg aaaagaagaa ttatcacttt
     6421 ataatgattc agaggaagaa gtaatagaat tagaagaaga aaaaagaagt tttatggaaa
     6481 atatgattga tggtcttaaa tggtcttttg gtgaattagc ccttactatt agtaaatatg
     6541 tagtaatggg tatggttttt gcaggattta taacaactat ttttccaaat agtataattc
     6601 aaaaatattt aggaaatcca ggtttgctat ctcttggaag tatagcaata cttgcatgtg
     6661 ttatgtatgt atgtgctgta ggacatatac cgtttatagc agcccttgtt gcaagtggag
     6721 catctcctgg tatagcgata acatttctaa tagctggagc agctacaaat ctaccagaac
     6781 ttataagcat gtttaaactt attggaaaaa gaactgttat tatatattct gtaactttga
     6841 cactgtcctc aataataatt ggatatataa caaatttact tttagtgaat aataattcag
     6901 taaatttaga tgcaacaaat tcttcaatac agttggcaaa taaactaatg ttaaatatac
     6961 ctgatagttt tgagtatgta tgtactattt taattatttt atttgcattt aaatcaacaa
     7021 taccaaagat gaaagaggtc ataggaaatc atgcttaaaa agatagttat aatatcctgt
     7081 atagtagttg tgttaatcat tttatcaaaa atagttgatg ataacatcaa agaagatgct
     7141 tctattccaa atgtgaataa agaaacatta gaatatttta gaaaaaatta taaagaagac
     7201 ataataactt gtgctgaaga agatttaaat aatgatggga aaaaagattt agtagttata
     7261 tataaaaaaa gcaataattc aaatgaaatg gtagtcgtag tttctgataa aaatagccac
     7321 tatataacaa agccaatacc agcacctata gagaatcaaa ctataacctt taaaaatatt
     7381 gatgataaag caccaataga agtaatagta tcagggtcta aaaatggtaa tgtaggatat
     7441 gctatttaca gagtagaggg taaaaaattt gttgatttat ttggcgaaga tatggacaaa
     7501 tgttgctaaa aataaaaata atacataaag gtgggaaatt tatatgaaat tatctaagaa
     7561 gattcaagca ctaatcttat ttggtgtaat gggtacatct atattaacag gatgtagtag
     7621 taaaccaaaa gaaaaagaag aagcaaaagc tggaaaagat gattatacaa taacagtagg
     7681 ttattataat tgtgaccata tgactgcggg accagtagca gaatcggcag gaatatataa
     7741 agatttaggt cttaatgtaa aaactgttgg aaatggtaaa gttcctgaag ctatggcagc
     7801 aggaaaaatg gatgcaggtt acataggaac taaggggtta gttggagcaa tacctaaggg
     7861 ttcaccaata acaatagcag ctaacaacca tactggtggt tctgaatatt taatagtttc
     7921 aaaagatata aaagaaccta aagatttaat aggtaaaaaa attgcaacag atatgagtga
     7981 tttcttatgg acaagtgatt atggtccaga aacaggtcta ccaactgacc catcaaaata
     8041 tgaagttgta aatatggatt cagacaaaga taaatattta gctcttaaaa ctgggaaaat
     8101 tcaagctttt acatcttgtg acccttgggg ttctgtagct gaaaatgacg gtgcaggaaa
     8161 aataatagca tctactcaat ataaagaaaa agctaatggc aaagaatata actgttgttc
     8221 attctcatta aacaaaaact ttataaaaga acatccagat ttagcaaaaa aattggttct
     8281 agcacataca aaagcaatag aatatatata tacaaatcca gctgaagcag ctaaaatatt
     8341 tgcaaaatat tataatgtag aagaagaagt tgcacttaga actatctata agaagactgt
     8401 tggagaaggt agaactctta cttggaaagt tacaggtgaa gaatataaaa ataatttaca
     8461 aatgtataaa gatttaaagg ctttagatga tatacctaaa tatgaagata cgattgatac
     8521 aagcttattg gactcttgtg gagctgatga ttttgacaaa tttataaaag aaaaagttaa
     8581 cccagagttt cctgaaggta tgagttatga agactggaaa gcaaaggtat tataaataaa
     8641 tggagaatat aaaaaatata ataatacttt ttatcattat tttgggaagt atatttataa
     8701 tattttttaa ttttgaagat aaagaagaaa taaaaggaaa attaagaata ggggtatctg
     8761 atgatacctc tggttttgta ataaattata tggtaaatag agactacttt gaaaatataa
     8821 aagtaagtga tataatggat atttttacaa ttaatgattg ctgagcaagt acaatgcagt
     8881 gggctctgag ttcagaggaa atagatatag caatcatatg taaagatgct gcaaaacaat
     8941 atgtaaatgt aaatagtgga tttgaaattg ttggtactgt agttcaaaat tctgatatat
     9001 ttctattaag tgataataat cctgaaaaga taggagtcat tcaaaataga gattaccagt
     9061 cagaattggt taaaaaatac tacaagaatg tggaagtagc tccattatta gggactgcac
     9121 tagcatatgg attagaaagt aatttagtag atggtgttgt aatagattgc ataaagtcaa
     9181 ttgggttaga gggtaaaaga aaatctacaa ctaaattagg agattatgat acctatgtat
     9241 tagttgtaag taaagagttt aaaaaaagta aagcatatag tgattttata acactttaca
     9301 ataaaagtgt tgatgcttta aagaataaag ctacatttaa gaaagcatta aaagagtaca
     9361 ggaatataag aaatatagat atggaggaaa taagcaattg gaaattaaaa tttctaaaga
     9421 taaaagcgat agattaacta agaaaaaaac tgatggtagt atggatattt tatataaaat
     9481 aatactatta gttattatat ttttagtctg gcattttgca gcaaaagata tagggagttc
     9541 tttgttactt ccaatgccag tagatgtaat aaaaggtttc tttttctgtg tgacagatgc
     9601 agagacagtc actaatcttt ttataacatt gcagagagta cttaaaggtt ttatgtatgc
     9661 attattgttt gggcttccaa taggatttat tatgggattt tctaaaactt ttgaaagggt
     9721 tttatctcca gtagtagatt ctgtaaggca agttccaata atggcatggg ttcctcttac
     9781 tatagtatgg tttggtatag gagatggtcc aacaatattt ttaatagctt tttcaggggt
     9841 tttccctata attttaaata ctattcaagg agtaagagca atatcaaagg attattataa
     9901 tgcagctaga agtatgggag catcgccaat tgtgatattt acaaatgtaa ttgttcccgc
     9961 atctcttcca gatatactaa ctggttcaag gattgctata agtacaggat ggatgtctgt
    10021 tatctgagcc gagtttatag ctacgagtgc cggtctcggt tactcaatgg tacaagctca
    10081 aacattaatg catacagatg tattaattgg gcttatgata tttgcagctt taataggatt
    10141 tataatagat agggtattaa agtttataaa taaaatcctt tgtagatgga ggtttgcaga
    10201 ttaatgaagt taagtgtaag agatataaat aagacatttg taaataatag agtacataca
    10261 aaggttttag aagatataag tatggatata gatgatggac aatttgtctg tcttttagga
    10321 ccatcaggtt gtggaaaaac aactttactt actataatag gtgggtttca aaaatcagaa
    10381 cgtggagatg tttttataaa tgataaaaga gttaaaaaac cgggcataga tagagctttt
    10441 atatttcaaa attatgcttt atttccttgg aaaactataa gaggtaatgt tctatttcct
    10501 atgaaacagc agaaaatacc aaaagaaaaa agagaagaaa tgctagaaga acttttggtt
    10561 atgtcagact tgaaaggaaa agaaaaactg tttccgcatc aattatctgg aggaatgaaa
    10621 caaagagtgg ccatgataag agctttagca tgtaatccag aggtattact aatggatgaa
    10681 ccattaggag ctgtagattt tcaaatgaga caaaatttac aggaagagct agaacgaata
    10741 tggataaaga agaaaataac tgcattaatg gtaactcatg atgttgatga agctgtatat
    10801 atgagtgata gagttatagt aatgtcgaga gataaaggaa gaattattga tgatataaat
    10861 atagatattc caagacctag aattagagga agccaaaaat atgaagagta caaaaacaaa
    10921 ttaactgata ctttatcaaa gtgttatgaa gtttaaagag gagtagacat gagtcaaata
    10981 gaaaaataca atagtttgga taatacacaa cagaaaataa gaatctctat tatggatatg
    11041 ataatagata aaggttctag tgttacactt caagaagtga ctgaatatgt aagtaaaaag
    11101 ttgaatatag aaaaagaata tatagaaagg acactacaat attttattta tgaaaatata
    11161 atggttgttg atgggaatag tataaatttt atatatcctg tatcagcatt gcaaacaaat
    11221 cataaagtaa ctttgagaga taatagaagt ttcagcgcaa tgtgtgctat agatgctata
    11281 ggaacctctt gcacattcaa ccaagatata aaaataaact ctatttgtag tgctacaggt
    11341 aaggaaatag aagtaattat aaaaaatgaa aagattgagt atgtgaataa tccaaactta
    11401 agagtgcttc atataaatct tgataagcat ctaaattggg ctgctagttg ctgaaatata
    11461 atgaatttct tctggacgaa gagtgatttt gatgcttgga caaacgaagc gggattatct
    11521 gatgatgaag atatatattg tttagatatt aatgaggcaa tagtagaatc atataaaata
    11581 tttaaactca aacaaaaggt actatcatag atatacatat atgaaaaact aaaaatgacc
    11641 tttagataaa aatgataggt aatatccata ctatataaat tagtatagag tagggatgtg
    11701 aaaagtattt aaatctaaag gtatattttt agttttttta gatgattaga tagccttatt
    11761 aagtatatat ttatttataa cctttgcaac tccattttca ttattagtat cagtaacata
    11821 atctgcaatt tcctttatac tattacgtgc attccccata gccacaccaa gacctgcata
    11881 ctttatcata tgttggtcat tttcttcatc gccaacagca atagcatttt ctataggtat
    11941 atttagattc gtacataatg ttttaagagc agttccttta ttagtagttt tacctaaaaa
    12001 ttctagataa aaaggagcag accttactat agtatattta ttgaataaat ttttaggtag
    12061 ttgctgtatg atttcttcca atctttctgg ttcatcaact atcataattt tacagatttt
    12121 aatagattca tcaattttat ctttttgtat atatttgact tctatattat ttaatttcgc
    12181 ttcatgaagt gtatattttc caggtgcttc atttggtgta attaaatttg tcattgtatt
    12241 tatgtgtatt ttagtcttat attttttaca aacgttatta tatataaaat caaaatcatc
    12301 taaagtcatt tctacatcat gtatgatttt ctcactttta acttcttgaa taattgctcc
    12361 attaaagcat ataacataat caccttcatc tgttaaattt agttgattta gaagttcttt
    12421 tacaccagga agaggtctgc ctgttgttat aactactttt gccccagccc tttttgcctc
    12481 ctgaatggac tcaaagactt caggagtaat ccttttttgt gtatttaata ttgttccatc
    12541 aatgtctagt gcgattaatt tgtacatatc aacctccaat aaattatata ctcaaatttc
    12601 ctgtgaaaac gttgacacag ctagtgttga acaaagtttc agtgggaaat atggtttgtt
    12661 ttgtatacta ttatatcaag agataagtaa tttagaaagt ttaataatat taatttttga
    12721 taaaaatatt acttatgtga gatgagaatg taaaaacttt gtaatcaata attccaaaat
    12781 tcttaattac aaataatggt atcgtttgca ttgttaatat aaataaatgt atattatata
    12841 gttagaagtt tgaataaata gactgatggt aatttgttat attagttagt tttgtgttat
    12901 aaaatattgt aattccatta atcatatgtt attattcaac atgattataa tataaagaat
    12961 atttaatata tataataatt tataaaaaag tattacatag tacatgtaaa tctatattta
    13021 taaatacaat aaagtaaagt tgtaaaggag tgctttaaat gaagttttca gagtttaagt
    13081 atgaaagacc caattataat agtatgaaaa aagagttttt gagttgtgta gaagatataa
    13141 ataattctcg taattataaa gagcaacaaa aaaatataca taagattaat ttattaagaa
    13201 ataaaataga aacgttgtca aatatagctt cgataagata tagtacagac acatttaata
    13261 aattctacaa agaagaaaaa aattattggg atgaatatat gcctttgtat gaagaattaa
    13321 attcacattt ttataatgct attgtaaatt caaaatttaa atatgatttg ataaaagagt
    13381 ttggagaaca atttttcaca atagtagagt attctttaaa aagtttttct aaagaaataa
    13441 taagtgaatt gcaggaagaa aataaattgt gttcagagta tactagactt ttagcttcag
    13501 ctgagataat gtttgatggg aatataagaa atttatctgg tatggggaaa ttcatgtatt
    13561 ctaagaatag aaaaacaaga gaattagcaa acaaagcata ttataatttc tttgaagaaa
    13621 atgaaactaa atttgatgac atatttgata aattggttaa acttagagat aagatatcta
    13681 aaaaactagg ttttgaagat tttgtagagc ttgggtacgt tagaatgatg agaagtgact
    13741 acagagaaga tatgataaaa aatgttagaa aacaagtatt aaaatatgtt gttccaatgg
    13801 ccaatgagct ttatgaaaaa caagctaaaa gaattggtct agaatattta agttacatag
    13861 atgaaggcgt ggaattttta acaggaaatg caagtttaaa gggagattca agatacataa
    13921 ttcaaaatgg taaaaggatg tattctgaac tgtcaaaaga aacaaatgaa ttttttgatt
    13981 ttatgttaga aaatgaactt atggatttag aaacgaaaaa aggaaaagga gctggagggt
    14041 attgtactta tataccagat tataagtcac cattcatttt ttctaatttt aatcaaactg
    14101 ctgatgatat agatgtgtta acacatgagg caggacatgc atttcagttg tatatgtcta
    14161 gatggattga tatgccagaa attaattttc caacgcttga tagttgtgaa atccattcaa
    14221 tgagtatgga atttataaca tggccgtgga tggatttatt ttttaaagaa gatacagata
    14281 agtacaaatt cactcactta tcatcttcaa ttaaatttat accttatgga gtaattgttg
    14341 atgaatttca acactatatt tacaaaaatc ctaatgtaga taagtcaaaa cgaaaagaaa
    14401 tctggagatt tttagagaaa aaatacttac ctcatagaaa atatggtgat aattcttttt
    14461 tagagagagg ttgttggtgg tttaaacaag gacatatatt taaaaatccg ttttattata
    14521 tagattatgt attagctcaa atttgtgcgt tacaattttg gaaaaaaatg attcaagata
    14581 gagatgcagg atggaaagat tatataaata tctgtaaagt tggaggaaca aaatcatttt
    14641 tagatattgt gagtatggga aatttgtatt ctccatttga tgatggctgt attgagtcta
    14701 ttataggtga tgttaaatct tggtttgatg aaataaatga cagcaaatta taaaaaatta
    14761 tgagaatttt attgtataaa agtgaaaaat taaaaaatat ataaaaagtg attatttgtt
    14821 gtgaaaatag tcttgttttt aactaaattg ataaaataaa ctaaaaaaga tttgtactta
    14881 aaaaaaaaag aaaatacaag aaatatgcaa tatcaacatt tggaaaaaaa agtaaaaatg
    14941 tatacaaaat acatataaaa aaactattgc aaattttata gaatgtttat atacttaagt
    15001 caacagagat agaaaaaagc tatttataga aaacaaagga taaattgctt aattttaaat
    15061 tttctgaaaa aaataataat ccggatgaag agtatgggag actgctttat aaaaagcgac
    15121 cgaagggaca agtttaatct tacttaccca aaagattaga tgaaattctc aggtaaaggt
    15181 gactgtactt ggacggggct ctgaaaatga ttttaacagg gaagacaaat tgggagattt
    15241 gtttttcctg ttttttattt atcaagtaat aaataaaagt ttgaaagaga gtaaaaaaat
    15301 gataataatt acgaataatc caaaagtaaa agaagaagtt caaggcagag aagttttgtt
    15361 taaagacaca acttatattg gaatcttaga agcaagtaga gatttgatac atgaaggtta
    15421 tgaactttta tctcatccac tctatgggag tgtaaaacca aatgaaacac cttacaggac
    15481 tgttatacta aagaaaggaa atcgtttgga tataaactct ttaaccttaa ttgaagaagc
    15541 aatcatcact gcaagtaaat ttcaggataa taaaaagact ccaaaatgga cagaaaatgt
    15601 ccaagacgat tttagagtaa ttgattatga tatattttat aatacaatac aaagaatgca
    15661 gtatgaataa aaaaatttgt taaaaagtta ttgaaacagc ctaaatatta aatctaattt
    15721 aatattaaat aaaatgagaa atttaattgg ggaggataag tcaaaatgga aaatgtgtat
    15781 gacttagtaa taattggttc aggaccagct gggcttgcag caggtcttta tggagcaaga
    15841 gcaaagttaa aaactctaat cctagaaaaa gacaagactg gtggacaaat tgtaataaca
    15901 catgaaattg caaattatcc agggtcagtt ccaaatgcaa caggaccaag tttaatagct
    15961 agaatggttg aacagtgtaa agagtttgga gcagaaatgc taagagataa cattgttgat
    16021 actgagctag atggagatat aaaagtttta aaaggggaaa aggcagaata tagagcaaaa
    16081 gcagtaataa taggaacagg agcaacacca agaaagattg gttgtcctgg agaaaaagaa
    16141 cttactggta aaggggtttc atattgtgca acttgtgatg cagatttctt tgaagatttt
    16201 gaagtatttg ttgtaggtgg aggagacagt gcattagaag aagcaatgta tttaactaaa
    16261 tttgctagaa aagtaacaat tgtacataga agacaaggat ttagatgtgc taagagtgtt
    16321 gaagaaaaag caaaagctaa tccaaagata gagtttttat tagatacagt aattgaagaa
    16381 atcaaaggtg atggaatatt agaatcagtt gtatttaaaa ataaagttac tggagaaact
    16441 catgaatact ttgcagatga agaagatgga acaatgggag tatttgtgtt cgttggatta
    16501 gatgctcaaa ctgatttatt caaaggtaaa gttgatatgg atgaaaaagg ttacataata
    16561 actgatgaag atatgagaac taacatacca ggcgtatttg cagcaggaga ttgtagatct
    16621 aagactctaa gacaggttgt aactgcaact aatgatggcg caattgcatc tatagtagct
    16681 gagaaataca ttgatgaaaa atttgggaac tagaatctgt aaattaagaa atagtgtctg
    16741 taaatttaat gaataatatt taaaattaaa ataaaaattt caagggggaa taagaaaatg
    16801 ttagatttag ataaggctac atttgaagaa gaagttttaa atgcagaagg ttttgtattt
    16861 gttgattttt ggagtgaagg ttgtgagcct tgtaaagctt taatgccaga tgttcataaa
    16921 ttagctgaga cttatgggga taaaataaaa ttctgtaaga tggatacaac aaaagctaga
    16981 agattagcta taaaacaaaa agtattaggt cttccaacta tggctatata caaagatgga
    17041 gaaaaagtag acgaagttac taaggatgat gctacagttc ctaatataga aaacatgatt
    17101 aaaaaatatc tttaatatgt tgataaagta ttaacattaa aagaaaaaat ctatttttat
    17161 ctgaagataa gaagaaaaag gt
//
LOCUS       DAEPLF010000067        17146 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEPLF010000067 DAEPLF010000000
VERSION     DAEPLF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.067"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(222..746)
                     /locus_tag="KR314_001872"
     CDS             complement(222..746)
                     /locus_tag="KR314_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0328258.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(811..1563)
                     /locus_tag="KR314_001873"
     CDS             complement(811..1563)
                     /locus_tag="KR314_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBH0328259.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDISKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1594..2892)
                     /locus_tag="KR314_001874"
     CDS             complement(1594..2892)
                     /locus_tag="KR314_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBH0328260.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQQGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREVNVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2909..5173)
                     /locus_tag="KR314_001875"
     CDS             complement(2909..5173)
                     /locus_tag="KR314_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBH0328261.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5428..6246)
                     /locus_tag="KR314_001876"
     CDS             complement(5428..6246)
                     /locus_tag="KR314_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBH0328262.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGIDEKIIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIIL
                     LDEPTAGLDPVSTRSIVDIIKGLNKDNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFVNENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6239..6937)
                     /gene="cbiQ"
                     /locus_tag="KR314_001877"
     CDS             complement(6239..6937)
                     /gene="cbiQ"
                     /locus_tag="KR314_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBH0328263.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIKNIFVLSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEIFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKMHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6940..7224)
                     /locus_tag="KR314_001878"
     CDS             complement(6940..7224)
                     /locus_tag="KR314_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0328264.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPDYEPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGVQKGKRS"
     gene            complement(7214..7966)
                     /locus_tag="KR314_001879"
     CDS             complement(7214..7966)
                     /locus_tag="KR314_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBH0328265.1"
                     /translation="MKQNIKLGVIAALMLIVLTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLVGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIISFLIFKALKKKDG
                     NNAMPVFLAAAIGDLATYTVTSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8155..8347)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8479..10251)
                     /locus_tag="KR314_001880"
     CDS             complement(8479..10251)
                     /locus_tag="KR314_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBH0328266.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYNMDKHPALDSMTRRIVNDIRNIDTN
                     QDMMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10265..11194)
                     /locus_tag="KR314_001881"
     CDS             complement(10265..11194)
                     /locus_tag="KR314_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0328267.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11423..11611
                     /locus_tag="KR314_001882"
     CDS             11423..11611
                     /locus_tag="KR314_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0328268.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRAKRRKEVQYE"
     gene            11604..12305
                     /locus_tag="KR314_001883"
     CDS             11604..12305
                     /locus_tag="KR314_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0328269.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLLDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSINNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12307..13230
                     /locus_tag="KR314_001884"
     CDS             12307..13230
                     /locus_tag="KR314_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0328270.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13387..14310
                     /locus_tag="KR314_001885"
     CDS             13387..14310
                     /locus_tag="KR314_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328271.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGCEKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDHTLRLYNTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14303..15040
                     /locus_tag="KR314_001886"
     CDS             14303..15040
                     /locus_tag="KR314_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0328272.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFASIALGRRK"
     gene            15051..15824
                     /locus_tag="KR314_001887"
     CDS             15051..15824
                     /locus_tag="KR314_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0328273.1"
                     /translation="MWKLLWAECQKIRRSKIVWITFFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16365..16973)
                     /locus_tag="KR314_001888"
     CDS             complement(16365..16973)
                     /locus_tag="KR314_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBH0328274.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5527 a         2979 c         2150 g         6490 t
ORIGIN      
        1 ttatttaaaa ataaatatat aaacaatcat aataaaattt ttttaataaa actttatttt
       61 ttagttaaaa tcatacaata tatagctttc cacaatacaa ataaattaaa aagtgaaatg
      121 tatttgtcta aaaacataat aacatttcac tttatcctca tttttaagta atatatattt
      181 ttaattttta tttataactc aatctagttt ttatataagt tttagttttt ttctacatct
      241 gagtaatcta ttccaaaagt ttctacagta acagacttca taatttgtgg agtttgtggc
      301 ttatcagctg aatctgttct aactgatgct atttcatcaa ctgtatccat accttctatc
      361 actctaccaa aacctgcata ctgaccatct aagtgtggtg agtttttatg cattatgaaa
      421 aattgagaac cagctgaatt aggtgccata gttctagcca ttgatatgac tcctctttca
      481 tgttttaaat tgttagtaaa tccattacca ctaaattcac cttttattga gtgacctggg
      541 ccacccattc ctgtaccatt tgggcatcct ccttgtatca taaatcctgg tattactcta
      601 tgaaatatta ttccattgta atatccttca tttactaatg atacaaagtt tttaactgta
      661 tttggagcta tatttggata taactcaatt ttgatttctt ttccattttc catttctata
      721 gttactatag gatttttatt ttccataatt tattaccttc cttttcatta gttaaattac
      781 ataattacat ttattatatt atactctaaa ttaaagtcta tcaagtatat tttttcctat
      841 ttcgtatcct tcttgataaa tagttttaca aaactcatca tcaaagttaa gcgtatcttt
      901 aggagcaaat tgagtattag gtctaactac tattatttta tcttcatcat atactttttt
      961 aatatcctct ggcaactcat aattatattt tgtggatatt aaaataattt tatccacatt
     1021 atccccaatt aatggttcaa ttaatgcact cctaattaaa cctccatcta atttgaaacc
     1081 atcataaatt ccttcttgca catctttcac aaattgttct ttaaatccaa gagttgcgtt
     1141 tggatttgat ggtaacaacc cactgtatga aatgctgata atggcttcat tacgaggcat
     1201 ttttgatatg tcaacaatct tttctgatac tactttatta tcaacttcaa cataattaac
     1261 ataaaagttc atttcttcta tattactatc atctagttcc cttaggttgt ctatagcagg
     1321 agaattatcc acagtattat ttactatttt tacatttcca ttctggatat cttttatatt
     1381 tgtccacatt ttctccaagt tatcaacatt ccctgtgtac aaataatatc catttattgc
     1441 tcctattgat gtccctgaaa tcgaataaaa tttatttatt cctccatcat acaaggcttt
     1501 aactactcct gcttgataag ccccttttgc tcctccacct tctaaacata atcctaattt
     1561 catagtctca tctccaaatt gtttatctta tatttatatt tcaaaaaact tactaaatgt
     1621 tttatatata ctatagtggt cctcaacatt tcctaattct cttatagagt gcattgctaa
     1681 aattggactt cccacatcca cagatggtat atctatatga gtagaagata ttggacctat
     1741 agtacttcct cctctttcat ctgacctatt aacaaattct tgatactcaa cattaacttc
     1801 tctacatata tttttataaa tagctataga atatccatca ctagtatatg cctgactagc
     1861 atttatcttt ataactggac cttttcccat tattggttta ttagttgggt catgcttttc
     1921 tggaatgttt ggatgaatag catgagctaa atctgatgat atcataaaag atgaataaat
     1981 agactcaaag aatccctctc tatccttacc cattgatatg caaattctct ccaatatatt
     2041 taacaacatg tttgaatctg caccttgttt agtagagcta ccaacttctt cattgtcaaa
     2101 tacagccatt acatttacac cttgttgacc tttagtatta acaagagcat tcaaactagc
     2161 atgtaccatt gcaagattat cttgtctccc aatagatata aattcatcat tagcccctat
     2221 taaacttcct ttttcaaatt catataaaaa taaatcaaaa tctataatat cttccatatt
     2281 tacatctaag ttagatgcta tttgctttat aagaaaatta tctttttcta atgtatcatt
     2341 taacatccca actaaaggta acatatcttt ttgcttatta tatgaatgac catcatttat
     2401 acttctattt aaatgtattg ctagatttgg tattatacaa attggctttt taaagtctat
     2461 aagcctttca tttggtttta atatactatc accttttaaa actactctac cagctatccc
     2521 aagaggtcta tccaaccatg tacttaatat tgctcctcca tagcattctg tatttaactt
     2581 caaataagat ttttcagctt ctatttctgc atttggttta atactaaatg taggagaatc
     2641 tgaatgagag cctattattc taaatccatc tctttctata tcttctgaat ttactacaaa
     2701 agctactata gctgatagat tcttagtaac ataatacttt ccaccaactc ttagattcca
     2761 tctttgattc aaaactaatt cttcaaatcc atttttatct aagatttcct tagtactctc
     2821 gactgcatga aaagacgacg gactatcata tatgaagtct attaagtttt tagcaaagtt
     2881 tatactatcc attcctcatt tacctccatt ataaaatatt tagtgttgta tattctttat
     2941 ttattttttt aattccctgt tggtcaaaag ctatagtaac atctgtacca attatagaaa
     3001 ccactgtccc aaccccaaat ttaggatgat gtaccttaga accaagtttt atatcatctg
     3061 gattagtttc tttattagaa tcttttattg tagcattaac tttatctgca actttagatt
     3121 tgcttatagt actcatatac tttttagtat atttatctaa catattataa cttgctttag
     3181 aatagcttag ttcttttttt actttgtaca atctttctat acattcttct ggcagttctt
     3241 ccataaatct tgaagctatt gcaacatttg ttttaccata taatgttctc ttctctgtca
     3301 atgacatata tagctcttct ttagctctag ttataccaac ataacaaagt cttctctctt
     3361 cttctatttg tgaatcactc atagatttta tagctcttga tataggaaat aacccttctt
     3421 ccatacctgt taaaaacaca acagggaact ctaaaccttt ggatgtatga attgtcataa
     3481 gcgaaaccct atcattgtct tcttcatcat ttgattcaga tgtaagtgct atacttgtta
     3541 ggaatgtctc taagtcttta ttttcataag tatcatcatt actactttca aactccaaag
     3601 ctatagaaat aaattctctc aaattatcaa ttctgtcttg agcttcttca cctttgcctg
     3661 ttaaatcttt ttcattttta tttcttatct ctacaagctc gtccatatat cctgtagtat
     3721 ccaagacttt ttctataagt ttgctaacag gataaacttc ttttatagtt ctaagagtcc
     3781 ctataatatc aacaaatcca cttatacttg cccttgcctt agtagatata tctgaatttg
     3841 tttctatatc aattaatact gagtatatac tttcctgttt taagttagct ctatcttcaa
     3901 ttttttctat agttctaagc cctatacctc ttctaggaac atttataatc ctttttatag
     3961 atatatcatc ttgtggattt tgaataactc ttaaatacgc caacaaatcc tttatttctt
     4021 ttctctcgta gaattttgta ccaccatata tattgtatgg aatttgactt ctatttaaag
     4081 cgtcctctac aggtcttgcc tgtgcatttg ctctataaag tactgcaaaa tccttataag
     4141 gtctattttc ttttctagct atttgtgcaa ttgaatcagc aatgaaatca gcctcttcta
     4201 tttcgctacc agttaattgt atctttataa gttctccttc ttttttttca ctccaaagct
     4261 tttttctttt tctttctata ttattagata taactttatt agctgcatct aatatcactt
     4321 gtgtagacct ataattttgt tctaacttta caacatgtac attgtcataa tctttctcaa
     4381 attcaagtat gtttcttata tctgcgcctc tccatccata aatactttgg tcatcatctc
     4441 caacaacaca aatattttga tgttctcttg ctaataattt tatcaattca tattgagctt
     4501 tacttgtatc ttggtactca tctaccatta tgtatctaaa tctacttctg taatatgcta
     4561 atacttcatc attagccttg aaaagttcta cagttttaag tattaaatca tcaaagtcaa
     4621 gtgcactatt tctttttaaa cggtcttgat ataacgcata aatatctgca atcttaacca
     4681 ttctattatc tgccatgttg atatctttaa actgctttgg agtataaagt ttgtcttttg
     4741 ctcctgaaat tgcacttata actgcttttg gttcaaacac tttatcactt aaatttaatt
     4801 cttttagaca gtctttaacc aatgtaactt ggtctgctga atcataaatt acaaaacttc
     4861 tattgtaacc aatcttattt atatcttttc ttagtatacg aacacagcaa gaatggaatg
     4921 tacttatcca catatcctta gtttcaggac ctatattttg ttcaactctc tctctcattt
     4981 catttgcagc tttgtttgta aatgttatag ctaatatatt tggtgcttgt acacctttat
     5041 cttctataag atatgctatt cttgttgtta ataccttagt tttacctgaa ccagcaccag
     5101 ccagtataag tactggacct tcagtctttt ctactgcttc tctttgagca ggatttaatg
     5161 tatctaaatt catatttctc ctcctgttaa cagccataaa tatattttaa tctaaaatat
     5221 gaattacttc aaattataat tatgtattat attaatagat tatttccaat atattacaat
     5281 taaataacat ttttccatat aatttattta attgtttaat aaaaaaaata accttatgat
     5341 ttggggaatc ataaggcacc tttaaggggt ctaggttaag ggttattata tataaatcta
     5401 tttttttaaa ataggtttag ggcttaatta tttatttgta ttaagttctc tttccttcca
     5461 atacttatac aaatcttctt ctttcttaaa taagggtaaa ttcatatttc tatgtacttt
     5521 tactaaccat ggggattcta ggccagcttg tataatgtta ttttcattta caaatacatt
     5581 ttctgccttt ccttcatcaa ttaaaatccc cttatccaat acatatatat aatcgcatat
     5641 ttcatacatt aaattcatat catgactgga tattactatt tttatattat ctttattcaa
     5701 tccctttatt atatctacaa tagacctagt acttactggg tctaaaccag ctgtaggctc
     5761 atcaagcaat ataatttcat tttccattgc tataactgat gctatcgcta cccttttttt
     5821 ctgaccataa cttaaaaaat gtactggtct atctataaaa tctattccat taacagctac
     5881 tagagctttg tttattctct ctttaattat tttttcatct ataccaatat ttctcattgc
     5941 aaaggctata tcatcatata cttttgaata aaaaatttgt ttttctgggt cttggaacac
     6001 aatacctaca ttttttctaa ggttgtataa acttctctta tcatatttta acttttcttc
     6061 tttaaaaaga atttctccag atgtaggctt taatataccc attaaattca taaataatgt
     6121 tgattttcct gaaccatttg aacctattat acctataaca tttccctttg aaaaatccat
     6181 attaatattt aatagtgcat tggtattctt ttcatattga taagttaaat tatttatttt
     6241 aaacatcatt atcccctact atgtgaaatt taccatcata caattttatg tctaatgata
     6301 tacacatatc atcatacctt gtcataagtc ttttgtacag catactgcca agaattccta
     6361 atgatttata tgaagttttt aaatttatgt acccaaatct tagttcttgt gacttatata
     6421 tttctgaaaa ttcttctaaa aatatgaata taaacctata tattaacatt gatagctcaa
     6481 caaaagtatc aggcaaatgc atttttttaa agaagaatat taattgatta aaaggtgttg
     6541 taagtataca aaagtataca caagtcaaac aagacatagc tctaaataac acatgaatag
     6601 acatgtcaat agatgctttt gatattccta cattgaaact aaaaatttca aaactatata
     6661 ataagtctgc cttattaaaa gatatattta caagagttat tcctatacta agaaataaaa
     6721 agtacattgg tattctgagt aattttaaat aattctttaa atctattcca gctacacaaa
     6781 caactagaat actcattaaa atcataattc cacttaaaac aaatatattt tttattacca
     6841 tagaagcaat cagaaagatt actcctatgg ctactttttt atttggattt actttactta
     6901 atctatttgt atatgcatat ttatcaatta taagcataat taacttctct ttcctttttg
     6961 aacacctaat atgtacccta ttacaccagc acctaacgct gcttgtgcag agaataataa
     7021 actttctatt tctccacttg gtggctcata tgggctacta aaccatggtt cataatctgg
     7081 atttatctta gtaatttcac tctcagcttg accatctgct cctccatatt cagctcccga
     7141 attaacaagt aatgggaata tgataagagc tactactaat aaaagtagta atatattagt
     7201 tttcgtcttt gttttagcac tcattagctt taactccttt atcattattt tctagtatgc
     7261 ttttttctcc attttctgta attaaattat atacaattac agtaagaata ccctctgcta
     7321 ttgctattgg tatttgagtc ataaagaaaa ttcctaaaaa cttaattgct gaagccatca
     7381 ctccacctga tgggtctggg aaagccagtg ctaactgtat cgaagttact gtatatgtag
     7441 ccaaatctcc tattgctgct gcaagaaata ctggcattgc attatttccg tcttttttct
     7501 ttaatgcttt aaatattaag aatgatataa ttggtcctat aatagccatt gaaaaagcat
     7561 tagctcctaa agtagttatt cctccatgtg ccaatagtaa tgcttggaat attagaacta
     7621 tagttccaag aacaaacatt acacttggtc caaacattat tgctccaaga cctactccag
     7681 taggatgtga acaacttcct gttactgaag gtatctttag tgctgacaat acaaatacaa
     7741 atgctccaca cagcgctaat aatactttct ttttagggtc ttgctttact atttttccta
     7801 ttgattttag tcctactaaa aaaaatggta taaatataac tccccatgct atagaccatt
     7861 ttactggtaa gtatccttcc attatgtgca ttgcattcga tgtaactgga gttaaaacga
     7921 ttaacataag tgcagctata acccctaatt tgatattttg tttcatttta ctatcccctc
     7981 tctccctttc taataattaa aatataaata atatatgtct gatttatttt aaaaataaaa
     8041 ataaaaaagc ctaactcgat ggctaggctt ctaattttcg ataatccaaa tacaaacaca
     8101 taaaatgaac atatctgtat aattattata agaaactttc tccatccccg aagaaattac
     8161 tttaatattt taggcaggtc tcctgactta gcttcttcct actcctcttc cttcccatga
     8221 gatataaaaa atatcatcat agtggctaac aaaagtttcg tctgcatcac agtagcgggg
     8281 gctgtaaagg aatttaacct ttttccctat taatcttgct taaagaacct ataatattat
     8341 atatttaatt tttaaattat tcttaaatca ttttctatat aaatcattgt ataccttttc
     8401 tgtgaaaatt tctacatttt ttataaattt tattcatttt tatattttta tgtatataaa
     8461 ttcttttttg taagtttatt aatttctaat tatcttcttt aaatacattg aaactacatc
     8521 agaaaatcta gttatatctc ttgaatatat aaaatcattt ccatatatta atctttcagc
     8581 ttcaagttct gattcttttc ctgtaaatac tcctagtact aatatatttt gctttctagc
     8641 ttttctcact tcattggctg tatctctcaa accaactata cccctataag ccttttctcc
     8701 tcttaatgtt ctctcactac tttttcctat ctttacatca tttggtcttc catcgctgag
     8761 aactatcagg attttatttt cttcatctct atttattaga ccactacata tagattttat
     8821 tgccaaacca tctctattat taccagcaca aaaatattca aatatatttt cactattttt
     8881 tatactatca tcataatctt taaatctttt taatattgta tagtctaaaa aactagaaaa
     8941 tcccatcact ctatttggta tgccaacttc tgttagagca tttgcaatta tgtaagcttg
     9001 aattgccaca tttccctgat ttctactttg agagccactt gcatccaata atatatcaat
     9061 aacgtatttc cctttttcat tttctatatc tttataaaat attttattat tattacttct
     9121 accaactctc caagctctat ttgctaatat agtaccacaa tcagaatata ctctactttt
     9181 agcattttct tctattaaaa ttcttgacag actttctttt aatctcttta tttgctgtct
     9241 atacattcta gcatgttctc taaattttcc taaattattt tctttttgtc ttgttacata
     9301 ttttagttga aatacattat tacactcact tctaagaact ccatctgtaa agtgtattct
     9361 acaaccttca tgaacatttc tacaattttg tgtctcaatt tttttaattt cacctttact
     9421 caaataagtt tttccataat aatgttctat ttttgaatat atcttatttg cagtgtcttc
     9481 atcaacatat ataactcggt ctgctgaatt gctacattta gaatcatcaa aatcccctac
     9541 accttctaca agcatggatg aagatattga atctatatca ctttcaataa tattttcttc
     9601 ttcatcacta taaagttctt catacatgaa atcagcaaat gtatcaaaat ttacttctac
     9661 actttctaca ttattttcat caataagact attttcttct ccatttaata tgtattcgaa
     9721 ataagttaaa tatataacat ctaatgcttt taaaatctcc atcatatctt gattggtatc
     9781 tatatttctt atatcattaa ctattcttct agtcatacta tctaatgctg ggtgcttgtc
     9841 catattataa agtacaaatg tgtatttaat tttatccata atattatcat tgtgtatttt
     9901 ggtaaaacgt gaaacaatat ctttataagc atttcttctt atgtcttcta tccctggcct
     9961 atctttaatg accttttctt caactattgt atttaatata acttgaatta gacccagtat
    10021 aatatctttg ttgtatcctt ttcttattct atttgtaaga tattttttaa ctatatccca
    10081 atctacatac tttcttctag cacctgcaat tattgcaaaa tatagaccta tttcttttga
    10141 agagtaatct ttttctgata tatctatact ttcattgtaa tcaccagcta ttgtccatga
    10201 taagttatta gctctatttt caaattcata atcatcatat atccatttca taatctcacc
    10261 tatattaaat atgacttatt ggaaatacat ctatactctc ccatttatta ggtattctag
    10321 ttttaattac atctccaacc atttctttct catatacatc aaaagtcttt cctgtaagtc
    10381 ccatattaat tgcaagagtt ggttttaatc ctcttcttat agtcttaaga gatgccataa
    10441 gaccacgtaa atcaatagct ttactagata tctctccatt ttgagacttc aattgtaaat
    10501 ctaaaaatat tcctgcaaag tgtattaatt tttcttcatc agcatctgaa aattcatttt
    10561 ttaaaatcat cattaactta tcttcttcta taggaggaat atctattacc ataaatctag
    10621 ataccagtgc ttcattaagt tcttttgttc ctgcatattc ataattcata gtcccaataa
    10681 atcttgttgc tggatgtaga ttgactctct catacccagg tacatcaatt attcttctat
    10741 agtctaaagc tgaatgtacc acaactatag catcattttt tgccatatta atttcatcaa
    10801 atactccaaa acctccatta atagcacact cataaactga gcctcttcta agtttaactt
    10861 cattgtctat aaatgtatct gtacctataa gtgttgaact gtctgtattt atatgaaatg
    10921 atgtattcca ctgaggtcta ttaaatattt cccctagatt atcagcaagt aaattttttc
    10981 ccgttgcctt tggtcccgat aataatatat tttcttcttc taaaatagct gctatacaca
    11041 tagacaagat atctttacca taaaaataag ttttagactt tgttactctg tattctaaat
    11101 ctttttctaa accataataa tttctaaaat acagtacatc ttcaataagt ttttcatcta
    11161 ccccttgcac ccttagatta tctattatat ccatattttt acccctttta tataaatagt
    11221 atttgtttca tttcttatca ccattttagc ataaaaggat ataagctaaa attgattttt
    11281 taatctcccc cattttaagt aaaaccatgc accattttat cactacaaaa ttaagagtac
    11341 tacttactga tatttcttga ttttgttcgt ctatacgtat ataatatatg ctgataaatt
    11401 atgaggaaag gatgattgaa atatggaata tatgtcttgc ccagaagtag caaaaaaatg
    11461 gggcatttct gaaagaagag tacaaaaact atgtgaagaa agtcgcatac caggagtttc
    11521 aaaactcggg tatatgtggc tgataccaaa gaacgcagaa aagcctgttg atggtagagc
    11581 gaaaagaaga aaggaagtac aatatgaata gcgttttgat tatagatgat gacaaagaac
    11641 tttgtgctct tatgaaaaaa tgtgttgagc aagaaaattt atctgctatt actgcacatt
    11701 gtggtgcaga aggtctacat ttgctcgata aaaatagaca cacactctct cttgtaattt
    11761 tagacataat gatgccagat atagacggtt ttcaagttct gaaaaaaatt agagaaacaa
    11821 gtaatattcc agtaattatg cttactgcta aaaatgacga ggatgataaa gtttccggac
    11881 tacgtttagg tgctgacgat tacctaacaa aaccgttcag cataaacgaa cttatggctc
    11941 gtgtcaattc tcttattcgt cggtatacta tgttaaaccc tgcttttata acagacacag
    12001 attgcataat aataaatggc atgattattg acaagacaaa ccgaattgtt tctatcaata
    12061 atattcctgt tcaacttaca agcaaagaat ttgatttact tgcttttctg gcttctaata
    12121 agggacgggt atttaccaaa aaacagattt atacacaagt atgggaagaa gaatatgctt
    12181 ttgacgatag taacatcatg tctttcatca gtaaattgag aaaaaagatt gaaccagacc
    12241 ccaaccatcc attttatatt ttgacagttc gtggtgtcgg ttatcgtttt aataaggagg
    12301 cttaacatga atacaagtgt ctatctactt ttgatattgg ctcttagcct gattattata
    12361 gcgtaccttg tttctgtcct gctacgtgtc cgaatacaac ttacacttat aaaagatgct
    12421 ttagaggata taaaaaatgg aaaccttaat cgccgtattt taacagatga aaacgacatt
    12481 acaaaacaaa tttgctatga tattaacgaa atagcaatca atagccaatt tcaacttatt
    12541 cagcaaaaac aatcagaaca agcctacaaa cagctaatga caagtctctc ccatgatgta
    12601 aaaactccac ttgcttcttt ggtcggttat ttggaagcag tagaaagtaa aattgttgta
    12661 ggagaggaaa aagacgagta tatccatgtt gcgtctaata aggctcatta cttaaagaat
    12721 tttgtagaaa atctatttga atgggtaaaa ttggattcta aggaacaagt ttttcatttt
    12781 gatatttttg acttgaatga attatcccgt aatattatat ctgattggat acctgtttta
    12841 gaaagcagtc attttgaata tgaatttgat ataccagaaa tagagtactt cttacgtata
    12901 gacgcaaatg catatacccg tatcattaat aacctattac aaaatattat tacccatagt
    12961 agtggcaaca aaatgacatt gagaattttt gaaaacaaag agcaagctca aattataata
    13021 acagataatg ggaaaggtat atcttcagac aatctcccac atatttttga aaggttgtac
    13081 caatgcgacc attcacgagc ttcaaaggga aatggtttag gcttggcgat tgcaaaggaa
    13141 cttattaacg ctcataaggg aaatattacc gccaacagca ctcccggtat gggaacagaa
    13201 tttacaatat tgcttcctaa agccctgtaa aagtacgagg ctttcttact ctaaaacaag
    13261 ataaaagcaa gattatggca aggttttggc aaggttcatg ttttataatt ctaattgtag
    13321 ttgattttga cgttgtatca actatctttt gtagaaacaa atttattaaa agtgaggaaa
    13381 ttatatatga atgattttgt gattgaaaca aagcaactta caaaaattta tgggaatcaa
    13441 acagtagttg acaaagtcaa tctccatgta aaaaaaggcc gaatatatgg tctgttagga
    13501 cgcaacggag ctgggaaaac cacaattatg aaaatgattt taggattgac ttcaatcacg
    13561 tatggcgagg tagatgtatt tggagagaat attaagggat gtgaaaaacg agtatatccc
    13621 cgtatcggtg caattatcga aacgccagga ttttatccta atctgacagg aacggagaac
    13681 ttggagattt ttgcaaaact tcgtggtaca gccgccccta attctgtaaa aagagcattg
    13741 gagattgtag gcttacctta taaaaataaa aagctgttta gtaaatattc acttggcatg
    13801 aaacagcgac ttggtattgc caacgctatc ctgcatgacc cggaactgtt agttttagat
    13861 gagccaacca atggacttga ccctattggt attgcggaga tgagaaagtt tattagggac
    13921 ttgagcgttg agcatggaaa gacaattctt atctccagtc atattctttc agaaatctca
    13981 ttgttggctg atgatattgg aattatcgac cacggtgttc ttttggaaga aagtagtatg
    14041 agtgaactag aaaagaaaaa cagtaaatat atcctgctac aagtttcaga tattccaaag
    14101 acatcactta ttttagagcg tcaatttaac acgaaggatt attccgttca agatgaccac
    14161 actttacgcc tttataacac tactctggat atgggtgaaa tcaataaagc acttgtaatg
    14221 cagaatgtaa ctgttattag ttctcaactt tgtaatgata ctttagagga ctactttaaa
    14281 aaaatcacag gaggagaggg cattgcttaa acttattcaa acagaatttc ttaaactgcg
    14341 tcgtaggaaa cttatttggc tgatgttgtt gtctgctctc attatgccgt ttttcgcact
    14401 actatacttc aattattttg gtaaaacagg tgttgagcca acaaagtttt ataaatggtc
    14461 tgcatttggt tatacacttt ggattatttt gcccgttgtt ttgggtatat tttgcaccat
    14521 gcttatacac gaagaaaacc agtatgatat gctcaaacag ctttggattg tacctattag
    14581 taaaatggga tactttttta gtaaattttt cgttgttttg atttactcta tttgttttat
    14641 gctgattaca gccgttgctt ctgtgttatt tagtgtactt tctggatatg tcgtatttag
    14701 ctgggcaagc attttatatt tgttgaaaaa atgtttagaa attggtgtaa tcactgcttt
    14761 tgtaatgttg ccgattttgg caatcgctac atctcaaaaa ggatacattc tcccaatttg
    14821 tatcacattg gtatatgcct tttccggttt tgttataatg acaataaata tgtatttaca
    14881 cccattatcc agtatgggag ttattatcat gagaaataaa gacattccag gtcttatatt
    14941 ttcacaggaa attaatgtcc cattagcatt tctttgtatt tgtatttgga gtattgtatc
    15001 agtattgttt gcaagcattg cactaggaag aagaaagtga ggtgtagata atgtggaaat
    15061 tactttgggc tgaatgtcaa aaaattcgtc gttcaaaaat tgtttggatt acattttttg
    15121 catccattat gatagcagtc atagtttttg caggagggca ggatgtatat aatgacccag
    15181 atttacatta tggcttaaag agcgtttatg acggttcacg gtatatagac aatgcaggtt
    15241 ggtatatgga cgcggtacaa ccttggtcaa ctttttttgt tcttcctgct gtcattgccc
    15301 tattaggaag ctatattatt tgtcgtgaag aagaggagga cactattaaa tccctgcgtc
    15361 ttattcccat aaatgaaaca aaattaacca tagcaaaaat gattattact tttatattca
    15421 gtatacttct ttatttgtta ctctttacta taacattttt gactgaatcc gttttacatt
    15481 tttccgattt atcgacaaaa ctggttttaa gttgcatgaa agagtatttt cttgatggca
    15541 taggtgtatt ctttgcaatt tctcccatta ttgccttggt atcgcgcatg aaaaaaggat
    15601 attggcttgc attagtattc acagaagttt attctattgc tggactattt gcgggtatgt
    15661 caaatgtctt acaaaccttt tttcctattg cagcaatatt taatatttct ggatactata
    15721 taactactgg agaaaaaatt atggggagta tcataagttt attactttgt gcctgccttt
    15781 ctgcatttat actgaaaggg ctaaaacaca atgaaaaaaa ttgaaatagg tatatcaaaa
    15841 tgaaaaagat aaaaaaactg attattctaa ttgtattctt gtttcttttt ggcatgttac
    15901 actgttatac tgaatggaaa ttcaacaatg acccacctaa aggggtaata gaaagaataa
    15961 atgagtggac tgataagatt ggactggatt agtaacttat gttaattata gtgaatgtaa
    16021 tattagcaag ttattgtttc gtattttaat actataactt taaatatttg gttttaatct
    16081 tccgaacgaa tataaaaaaa ctgtcacaga ggcaacatat ttttgaatat ttataaagtg
    16141 gtagttttga ttttcaaagc tgccgccttt tcttctatat aagcaaactt atcctcaaca
    16201 aagtgtaaaa catggtacat tttaagaaaa aagtaccaca gacacaagca ataaaagaaa
    16261 cgccgagaac ctacaaaaac gtagtgctta tgcagattta cgacgccttc tattttctca
    16321 accgtcttaa tgaccttgta caaaagaaat ttttctaata ttttttataa tcttatcttg
    16381 tgatgttttt tatgtataaa tgacttaata acaaatccaa taaaaattat ggatattggt
    16441 ataattagaa cgaggaattt ataatcatta taatgtgaca ttattcttac ccaactctca
    16501 gaaaaaatat atcctatact gattaatgaa gtattccata tggttattcc cacgatagat
    16561 gatattacaa aagttataat attttgacct gctatacctg ctataaaaga tatgtatgtt
    16621 ctacataaag ggattaatct acatacacaa acagaaatag aagagtattt attaaaataa
    16681 tcttgagatg caaatattcc tttctttgcc tttggacata tctctattat tttatttatg
    16741 atagatttac ctccccaatt tcccactgta taacaaaata tcgaaccaag tatacctgca
    16801 attatactta aaattaaaat tgtcagaaat ccaaaatgat ttcttgcagc aattgctcca
    16861 cataaaggta aaacaacttc gctaggtaat ggaaaacaag catactcaat cataattaat
    16921 acaaatacag agattaatcc atattgtaaa ataaaactat tgattacaga cacttactta
    16981 tctatctcct ttcttattta aaggttttaa gggtacatat tctaaggatt ctcaccctat
    17041 atttaaagaa tcaatctctt taaactatta tatgtattta cacaaagcat ttataacata
    17101 ctttaaaaat agtaccttaa aataagaaac ttaataaata aaaaaa
//
LOCUS       DAEPLF010000068        16545 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEPLF010000068 DAEPLF010000000
VERSION     DAEPLF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.068"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            202..1332
                     /locus_tag="KR314_001889"
     CDS             202..1332
                     /locus_tag="KR314_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH0328275.1"
                     /translation="MNNFEIKTRIKFGEGSLKVLQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKSIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILNNIK"
     gene            1573..1923
                     /locus_tag="KR314_001890"
     CDS             1573..1923
                     /locus_tag="KR314_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBH0328276.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKRD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            1931..2380
                     /locus_tag="KR314_001891"
     CDS             1931..2380
                     /locus_tag="KR314_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBH0328277.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDDIGLEKLFKYLEKSKNIDEV"
     gene            2482..3057
                     /locus_tag="KR314_001892"
     CDS             2482..3057
                     /locus_tag="KR314_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBH0328278.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            3050..4459
                     /locus_tag="KR314_001893"
     CDS             3050..4459
                     /locus_tag="KR314_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBH0328279.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSNENGTEVLITIPTQINI"
     gene            4643..6076
                     /gene="eutA"
                     /locus_tag="KR314_001894"
     CDS             4643..6076
                     /gene="eutA"
                     /locus_tag="KR314_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBH0328280.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQTIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSVDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            6094..7458
                     /locus_tag="KR314_001895"
     CDS             6094..7458
                     /locus_tag="KR314_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBH0328281.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFYKDGKLTELAGDAS
                     VLLG"
     gene            7471..8352
                     /gene="eutC"
                     /locus_tag="KR314_001896"
     CDS             7471..8352
                     /gene="eutC"
                     /locus_tag="KR314_001896"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBH0328282.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKTTEVVVDNNAFI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSQETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            8372..9025
                     /gene="eutL"
                     /locus_tag="KR314_001897"
     CDS             8372..9025
                     /gene="eutL"
                     /locus_tag="KR314_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBH0328283.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEIAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            9036..9737
                     /locus_tag="KR314_001898"
     CDS             9036..9737
                     /locus_tag="KR314_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBH0328284.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRIDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEITDNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            9740..11209
                     /locus_tag="KR314_001899"
     CDS             9740..11209
                     /locus_tag="KR314_001899"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBH0328285.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNIPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            11305..11592
                     /gene="eutM"
                     /locus_tag="KR314_001900"
     CDS             11305..11592
                     /gene="eutM"
                     /locus_tag="KR314_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBH0328286.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            11719..12480
                     /locus_tag="KR314_001901"
     CDS             11719..12480
                     /locus_tag="KR314_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HBH0328287.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLEDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            12493..13122
                     /locus_tag="KR314_001902"
     CDS             12493..13122
                     /locus_tag="KR314_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBH0328288.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            13164..13889
                     /locus_tag="KR314_001903"
     CDS             13164..13889
                     /locus_tag="KR314_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBH0328289.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEILEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            13902..14174
                     /locus_tag="KR314_001904"
     CDS             13902..14174
                     /locus_tag="KR314_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBH0328290.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            14167..14718
                     /locus_tag="KR314_001905"
     CDS             14167..14718
                     /locus_tag="KR314_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBH0328291.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYVVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            14730..15821
                     /gene="eutH"
                     /locus_tag="KR314_001906"
     CDS             14730..15821
                     /gene="eutH"
                     /locus_tag="KR314_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBH0328292.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NSGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLRKENV"
     gene            15823..16296
                     /locus_tag="KR314_001907"
     CDS             15823..16296
                     /locus_tag="KR314_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBH0328293.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(16416..16517)
                     /locus_tag="KR314_001908"
                     /pseudo
     CDS             complement(16416..16517)
                     /locus_tag="KR314_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
BASE COUNT         6812 a         1865 c         3078 g         4790 t
ORIGIN      
        1 attaaatata agtaaagcta atacataagc tttgattatt gattttatat actattggtt
       61 ttatacgcta tgacttgaca ttatattaat caaatagtat atttttaaat atagcacaat
      121 gaagtgttaa aagagcaatg gagctccata gataaagtct atggagcttt tttgcgtatt
      181 taacttaagg aggatagagc tttgaataac tttgagatta aaactagaat aaaatttgga
      241 gaaggttcac taaaagtttt gcaagaatta aaaaacaaaa atgtattaat aataacagac
      301 ccttttatgg tgaaatcaaa aacaatagat aaaataacat ctaatttaat aaactcaaca
      361 tatcaagttt ttgaagaaat agtaccagac ccaccaatag aagtagtagt agcaggaata
      421 gaagcattta aatctataaa tccagacgta ataatagcat taggtggtgg ttctgcaata
      481 gatgcagcaa aatctataat ggacttttct aaaaaaatat taaaaattaa cgaagtagag
      541 tttatagcaa taccaacaac aagtggaaca ggttcagaag taacatcatt tgctgtaata
      601 acagatagtc aaaaaaaatg taagtatcca ttagtgagtg atgatttact tccagatatg
      661 gcaatactag acccagagct tgtaaaaact gtaccaaatt ttataacagc agatacaggg
      721 atggatgtat tgacacatgc tatagaagct tacgtatcta caaattctaa tgacttttca
      781 gatgcactag ctgaaaaatc tataaagtta gtatttaaat acttaataaa agcatataaa
      841 gatggaaatg acatagaagc tcgagaaaaa atgcacaatg catcttgttt agcaggtatg
      901 gcatttaacc aagcatcact tggcataaat cattctattg cacatgtatt aggtggaaaa
      961 ttccatatac cacatggaag aacaaattct atattacttc ctcatgtaat agaatataat
     1021 tcatcaatag taggatatac taacacaact tactctgata cagctaaaaa atatgcacaa
     1081 atagcagaat tagtaggact tgggagtgct aatataagaa tatctgttag aaatttagta
     1141 aatgaaataa agaaactaca aaaagaaatg aatatgccaa ctaaattgac acaatgcaaa
     1201 ttaagtttag aagatataac taatgaagaa agagaaatag caaaacttgc tataaaagat
     1261 acttgtacat taactaatcc taaggtacca actgaagatg atataataaa catattaaat
     1321 aacattaaat aactacattt tattacatgt aaatataata ttatacaaat agggaaaatg
     1381 tttttaaaaa tataaattat atattgacaa atataaaaca taaaactata atgaaagtat
     1441 cacaaaggcg tgatacttta gtagaaagca atggagcttc ttaggaatat taatattatt
     1501 cttatggagc tccttttatt ttaagcaaat agctttttaa taaactatat tcaaagaaag
     1561 cagggaataa atttgactga agaatctaag caaagagtta ttcaagaata tgttcctgga
     1621 aaacaggtta cattagcaca tatcatagct aaccctaatg aagacatata taagaagtta
     1681 ggattagtac ttgataaaag agatgcaata ggaatactta caataacacc aagtgaagcg
     1741 tcaataatag cagcagatgt tgctacaaag gcttcaaatg tatctttagg ttttatagat
     1801 agatttagtg gttcagtagt aatatcaggt gatgtaagtt ctgtagaatc agcgttaaat
     1861 gatgttttag aagtcttagg aaatatgtta aacttctcat ctacaaaaat cacaaggaca
     1921 taataataca atgaagaata taatatttat gggaaagaca ggtagtggta aaactacctt
     1981 atgtcaaaaa ctagaccagt tagaattaaa gtataaaaaa actcaatcag tggaacttta
     2041 taataatgca atagatactc ctggtgagta tatggaaaat agaaacctat ataatgcctt
     2101 aataacaaca tcagcagatg ctaaaattat agcaatagtg tatgatgcca cacaagaaga
     2161 aaattatata gcaccaggtt ttgccagtat gttttgcaaa gacgtaatag gaattataac
     2221 taaaataaat aaagctactg aaaacgaagt caatatagct tatgaaagac tatcactagc
     2281 aggagcaagt caaatcttta aaatagacac agttgatgat attggacttg aaaaattatt
     2341 taaatatcta gaaaaatcta aaaatataga tgaagtttaa ggggaaatat ttttttattc
     2401 aaaaatatta ttaaagtatg ataaaataaa aaaatgtata aactatttta agaagattta
     2461 atctttaagc gaggtaggaa catggggaaa aaaatactat tggttgatga tgaaccactg
     2521 ttaaggatgg atacaaaaga tatattagaa agtcatggct atgaaataat aggcgaagca
     2581 agtgatggat ttgaagctgt tgaaatgtgt aaaaaacaca aacctgattt agttataatg
     2641 gatatagaga tgccaatact tgatggtata aaggcaggca aaataatatg taaagaaaat
     2701 ctagctggtg gagtactttt attaacttca tttgaagata aagaatacgt agaaaaggct
     2761 aaaagtatag gtgcttatgg atatttagta aaaacagcaa gtgaaaaatc acttatccca
     2821 accatagaaa tgtgtttaaa taaggtaaat gaatttgaaa aaatgaaaaa agatttagaa
     2881 aaagtaaatt ctaaattgaa tgaaagaaaa ttagtagaaa gagcaaaagg tatacttata
     2941 agagagttta agatttctga ggatgaagca tacactagaa taagaaaatt aagtatggat
     3001 aaaagaacta caatgatgga aattgccaaa atgattataa taggatacga tgactaatac
     3061 gataaaaaaa atatcaactg cacaaacata cttaaataat aacgaaattg aaaaaattat
     3121 agaagtatca aaatccttag atttaatggc ttctttttat gaggctgatg tttttataga
     3181 tgtactaagt aaatttgatg atgaagcaat tgttgtggca catggtagac ctaaagaaaa
     3241 gtcaatgtac tgtgaaaatg ttataggtaa aaaggcttta attgaaaatg aaccaggagt
     3301 tataaagtgc ttaaaaacag gagaaagagt aagagatatt aaagctttaa cacaggaata
     3361 taaactggtc aaacaaaata ttcagccaat aacactagat gacaaagttt tagcagtact
     3421 aatagttgaa aaagatataa gtagtgaagt taagaatgag tttaatgcta gtgaagacga
     3481 agaaaataaa aatttacatc agtttataaa acttatcgga aataatgact ttttagttga
     3541 taatttaaat tcagccatat taatatttga taaaaatgga aaattaaaaa ttaaaaatcg
     3601 tattgcaact gagatataca aaaatcttgg gtttggatat gatatacaag atatgcatta
     3661 caatgattta gatatagaaa ccaacacctt tgaaaatata ttaatggatg aaaattatga
     3721 tgagacaaat gaagtttcaa taggtaatat gttttttaaa ataaagacca tatacatcaa
     3781 agatgatgaa tttaaggttt gcaaaatagt acaagatata agtgatttaa agagaaaaga
     3841 agaagaatta gttttaaaaa cagtggctat aagagaagct catcatagag taaaaaataa
     3901 tcttcataca gtaatatcta taataaaaaa acaaagtaga ttatctcaaa atgatgaagt
     3961 aaagcagtgt ttagataaca taataaacag agtatttgca atactttcaa ctcattattt
     4021 actatctaaa gaagttgata ataatatatc tatacgagaa ggtatcaatt tattagtgtc
     4081 aaatattcaa ggtggctatt tatttagtaa ggatatagat atacgtataa ctggtgaaga
     4141 ttttaaaatt agtggagaaa aagcaacagc aatacttttg gttataaatg aaatactaca
     4201 aaattgttat gaccatgcat tttcggatag agaacatggt actatacaaa tattaataaa
     4261 taaagataac gactatagaa atattgctat tatagatgat ggaattggat ttgatagtaa
     4321 taatataaat aaaaatagct tagggacata tataatagat agttatataa cacagatgtt
     4381 aaaaggaaat atagagagaa aaagtaatga gaatggtaca gaagtattaa ttactatacc
     4441 tacacaaatt aatatttaaa taataactca tctacaaagt agtagtttta attgagcaaa
     4501 gaggcttaag gttattaata gattaagtta attgaatgta tttataaaaa ttataagtat
     4561 atattcaatt taacaggtat ttattataac tttaagcctc tttgcttgtt taagacatac
     4621 ttaaatttta ggaggtgaga aaatgagaga agagttatta agtgttggta ttgacattgg
     4681 aacaagtact actcagttaa ttttttctaa attaatagta gaaaatactg aatcaaattt
     4741 ttcggtacct agaataagta tagtagacaa agaaattatt tataaaagca aagtttattt
     4801 cacccctctt atttcaaata atgaaataaa tggtcaacaa ataaaagata tagttgaaaa
     4861 tgaatataaa aaagcaaata tatctaaaga ggatatacat acaggagcag taataataac
     4921 tggtgaaaca gctagaaaag aaaatgcaaa tgatgtactt catactttga gtggatttgc
     4981 tggggacttc gttgttgcaa cagcaggacc tgatttagaa agtataatat caggaaaagg
     5041 agctggagct catatttatt caaaagaaca ttcaactaca attgtaaata tagacatagg
     5101 tgggggaact agtaacatag cctcatttaa aaggggagaa gtaaccgata ctgggtgttt
     5161 ggatataggt ggaagacttg tgaagataga taaaataagt aaaaaaataa cttatatatc
     5221 acctaaaata gataaaatca taaaagataa taatttaaat cttcaagtag gcacaatggc
     5281 tactgtagaa aatacaaagc caatagtgga tataatggtt gacttattaa tggaaagcat
     5341 aggattaaag aaacagacac ctttgtttga ttttataata accaataaat caataagtat
     5401 agatgaaaat attaaaaatg tttcattctc tggtggagta gctgattata tttactatga
     5461 tggagaaata aaagattatt ttgaatatgg tgacttggga attttacttg ggcaaacaat
     5521 aaaaaattgt gaatacctta aaaaagtaaa tgtagttaaa tctatggaaa caataagagc
     5581 cacagtggtt ggggcaggtt ctcatactac tgaagtaagt ggaagtacaa taacttacac
     5641 tagcgaaaag tttccactta agaacttgcc agttttaaaa ttatcagttg atgaagaaac
     5701 aggtacttca aaacaaatca gtgaagctat aaaaaataaa cttaattggt ttaaactaga
     5761 aaatgatttg cagaaagttg ctatagccat gataggaaag aaaaatccaa cctttttaga
     5821 tatacaagag tatgctaaag ggtttgtaga tggaatgcaa caaatcatag aaaatgattt
     5881 accttttata gtaattgttg aaaatgatat ggcaaaagtg ttaggtcaag caatatatgc
     5941 attattaaat tatgaaaaag aaattgtttg tatagacagt gtcaaggttg aaaatggaga
     6001 ctatatcgac attggaaaac ctatagttga aggtaaagtt ttacctgtgg taataaaaac
     6061 attagttttt aattaataga aggaggtaag taaatgattt taaatacaaa attatttgga
     6121 cgtacttatc agtttaaagg cttatatgat gtaatggcta aagctaatga agaaaaatca
     6181 ggagataaat tagcaggtct tgcagcagaa tcagctgaag aaagaatagc agcaaaagtt
     6241 gttttatcac acataaaatt ggaggatata agaaataatc ctgcagtggc atatgaagaa
     6301 gatgaagtaa caagaataat acaagatgct gtaaatgaaa aagtttatga agaaataaaa
     6361 aattggacag tatctgaact tagagaatgg ctattggatt caaatactac aagtcttgat
     6421 ataagaagaa tatcaagagg attaacttct gaaatgatag cagcagttgc aaaattaatg
     6481 tcaaatatgg acttagttta tgcagctaaa aagataaagg ttactgctca ctgtaataca
     6541 actataggag aacaaggaac attatcagca agacttcaac ctaatcatcc aacagacgac
     6601 ccagatggaa tacttgcatc acttcttgaa ggtttaactt ttggtatagg agatgcagta
     6661 cttggattaa atccagttga tgactctgtt gagagcgtta ctaaagtatt aaaaagattt
     6721 gaagaagtaa aacaaaaata tcagatacca actcaaacat gtgtattggc acacgttact
     6781 acacaaatgg aagcaataag aaatggtgct cctgcagatt taatattcca atctatagca
     6841 ggttctgaaa aaggaaatga agcatttgga tttaatggcg acactataga agaagctaga
     6901 cagttagctt taaaacacgg tacagctaca ggacctaacg ttatgtattt tgaaactgga
     6961 caaggttcag aattatcttc agaagcacat catggaatag accaagttac tatggaagct
     7021 agatgctatg gatttgctaa aaagtatgaa ccattcttag taaatacagt tgttgggttt
     7081 ataggacctg agtacttata tgatgctaga gaagttgtaa gagcaggact tgaagaccac
     7141 tttatgggta aactacatgg cttaccaatg ggtgttgatg tttgttacac taaccatatg
     7201 aaggctgacc aaaatgatat ggaaaactta gccatactat taacaacagc aggatgtacg
     7261 tattttatgg gaatacctca tggagatgat gttatgctta actatcaaac aacaggatac
     7321 catgaaacag catcacttag agaaatgttt ggactaacag caataaaaga atttgatgct
     7381 tggatggaaa agatgggatt ctataaagat ggtaagttaa cagagttggc gggagacgca
     7441 tctgtattat taggatagga ggtatttaat atgaatgaga aggatttaaa agcattggta
     7501 gaacaattag ttggacaaat ggttggagaa ttagatacta atgttgtatc tgagactgtt
     7561 aaaaagacaa ctgaagttgt agtagacaac aatgctttta tagatgatat aacagaagtt
     7621 gatataagaa agcaattact agtaaaaaat cctaaggatg cagaagctta tttagatatg
     7681 aaagcaaaaa cacctgcaag attaggtata ggaagagctg gtactagata taaaacagaa
     7741 acagttttaa gatttagagc agaccatgct gctgcacaag atgctgtatt ctcttatgta
     7801 gatgaagaat ttataaaaga aaataatatg tttgcagtgg aaactttatg taaagataaa
     7861 gatgaatact taacaagacc agatttggga agaaaatttt ctcaagaaac tataaataat
     7921 ataaaatcta aatttggtac aaatcaaaag gttttaatat tagttggaga tgggcttagc
     7981 tcagctgcaa tagaggcaaa tttaaaagac tgtgtgccag ctataaaaca gggtctaaaa
     8041 atgtatggta tagattcaag tgaaatttta tttgttaaac attgtagagt tggtgctatg
     8101 gaccacttag gtgaagaatt aggttgtgaa gttatatgta tgttggttgg agaaagacca
     8161 ggattagtta ctgctgaatc aatgtcagca tatatagctt ataagccata tataggaatg
     8221 gcagaagcaa aaagaacagt tatatcaaat attcacaaag gtggaacaac tgcagttgaa
     8281 gcaggtgctc atatagctga acttataaaa actatgttag ataaaaaagc ttcaggaata
     8341 gaccttaaat aaagtcgagg gagtgtagaa tatgaaaaat gatgtaattc gtcctaatat
     8401 actaggaatt aaaataattt cgaatataag tccagaaatg gcacaaaaac tagaattaaa
     8461 atcacatcac aaaagtttag gtttaataac tgcagactgt gatgatgtta cgtatacagc
     8521 tcttgacgaa gcaactaaag cttcagaggt tgatgttgta tatgcaaaat caatgtatgc
     8581 aggtgctgca aatgcttcaa caaaacttgc tggtgaagta ttaggaataa tagcaggccc
     8641 aagcccagca gaagtaagaa gtggtctaaa tgcagtagtt gattttctag agtatggagc
     8701 aacttttata agtgcaaatg atgatgattc aatagcatac tatgctcact gcgtatcaag
     8761 aacaggtaca tacctttcag agatagcagg aataagagaa ggtgaagcat tagcttattt
     8821 agtagcacca ccacttgaag caatgtatgc actagatgca gcaatgaaag ctgcagatgt
     8881 taagatgtgt gaattatttg cacctccaac tgaaacaaac tttggtggag cattacttac
     8941 aggttctcaa tcggcttgta aagcagcatg tgatgcattt gcagaagcag taaaatcagt
     9001 tgcagacaat ccaacaggat tttaaggata gttagatgga tgctataggc ttaatagaag
     9061 taataggata tgttgctgca atagaagctt ctgatgcttg tttaaaatct gcaaatgtta
     9121 atattgtaag aattgataag gttggggcag gtattgttac tttaacaata tgtggagatg
     9181 tcggtgctgt aaaagctgct ttagaagcag gtgaaatagc agcttccaga gttggtacat
     9241 taagaacatc acatattata ccgagaatcc ataatgaagt aacaaatgct ttattcaaaa
     9301 ctaaagaacc taaagtctgt gaagtttcag aagataaaga aaaaaacttt gagattacat
     9361 tggaaataac tgataatgta tcgaaaaatt tatcagaaaa agaaaatgta attttagatg
     9421 tcaatgaaaa cagtggagat actgaaaatt tatatagtga tatagataaa acaaacaaaa
     9481 aaattgaaac agttgatgat agtttaacag aagctaatga ggaaaaaata gaaaatacta
     9541 atgataaaga agtattggaa caagttaaaa tagaaaattc cgattttgat agagatgacc
     9601 tatcaaagaa cagtgtaaaa aaacttaaag ctatggctaa gaaattagat tcttcaataa
     9661 cttataaaga tttaaatgtt cttaaaaaag aagaattaat aaacttagtt aaaaagctta
     9721 gcagaaggga tagatagtta tgacactttt agataaagat ttagtatcta tacaagaagt
     9781 cagaactctt gtaagaagat ctaaagtggc tcaagagaaa ctttctaaaa tgagtcaaga
     9841 gcaaatagat aaaatagtaa aagctgttgt agatgcagca tttaataatg ctactaaatt
     9901 agctaaaatg gcaaatgaag aaactggatt tggaaaatgg gaagataaag taattaaaaa
     9961 tacttttgca tctatgggag tttatgaaac tttaaagaac acaaaaacta ttggaataat
    10021 tggtgaagat aaagaagcca atacgataga tatagctaca ccagttggag ttgtggcagg
    10081 tcttatacct tcaacaaacc caacatcaac agtaatatac aaaactataa tatctctaaa
    10141 agcaggaaat actatagttt tctctccaca tccaaatgca aaaaactgca tacttgaaac
    10201 agttaatata ataagtgagg ctgctataaa ggctggagct ccagaggatt caatattatg
    10261 tataactaat ccaagtatag ttggaaccaa tgaattgatg aaacataaag atacaaactt
    10321 aatactagca actggaggag aagcaatggt tagagctgct tactcttcag gaacaccagc
    10381 tattggagtt ggaccaggaa atggaccagc atttatagat aaatcagcaa atgttaaatt
    10441 agcaatcaaa agaataatag attctaaaac ttttgataat ggtgttatat gtgcatcaga
    10501 gcaatctata gtaatagaaa aatctatgga agaaactgtt ttaaaagagc ttaaaaatca
    10561 aggtgcatat gttttaaatg aagaggaaag tgaaagatta gcaaaattca tattaagagc
    10621 taatggtact atgaatccac aaatagttgg taaaagtgtt gaggtaatag caaatttagc
    10681 agacttaaca aatataccta aaaatacaaa agttctagtt gcaagagaaa ataaagttgg
    10741 tcacaatgta gcctattcaa gagaaaaact tacaccaata ctagcgtttt atgttgaaga
    10801 aaatgtagaa gctattatga acagatgtag agaaatattg ttaaatgaag gtgcaggtca
    10861 tacattctca atgcatgtaa atgatgaaga attggtaaaa agatttgccc ttaatatgcc
    10921 agtatcaaga atacttgtga atactccttc agcacttgga ggaataggtg catcaacaaa
    10981 ccttgtacca gcactaacac ttggttgtgg tgcaataggt ggaagttcaa cttcaaacaa
    11041 tataggacca cttgatttaa taaatgttaa aagagtagct tatggaatca aagaaataga
    11101 agaacttgga agagataaat ctcaagttga aaaatcaatg tttgactcaa tggataaaga
    11161 agaattaata aatcttcttg ttcaaaaact agtcaaagaa ctagcataac taaaataaac
    11221 aatgaaactt atggctatcg ctataaattt ataaaaatat aaactataaa aaactataaa
    11281 attaaatttt tggaggataa gattatggca agtgcaaacg cattaggaat gatagaaact
    11341 aaaggattag taggagcaat agaagcagca gatgcaatgg ttaaagctgc aaatgtacaa
    11401 ttagtaggaa aagagcaagt aggtggagga ttagttactg taatggtaag aggagatgtt
    11461 ggagctgtta aagctgctac agatgcagga gctgcagctg ccgagagagt tggagaacta
    11521 atatcagttc atgttatacc aagaccacac tttgaagtag atgctatatt accaaaggta
    11581 tcagctgagt aatataagaa taattttaaa atttaaattt tgattgaaaa agaggctatc
    11641 tcaaaaatga gtcttactaa taaactcaat gtattttgag gtagtctttt ttatacatct
    11701 aataggagat gtgataaaat gggtgtttta acagaaaacg aagtaaaaag aaaaattgga
    11761 aaaaacaaaa ataatataac tgaattttat atagaaaaag gtcaaataat aactccttct
    11821 gcaaaatctt atttagctga gagaagtatt gagcttaaat atgttgataa actttcagat
    11881 gtaaacaatg aacctaaaga agttgtaaaa gaagtagtta aagagaaaaa atataaatat
    11941 actactgtat ttggcgctaa attggatgaa aaaccagaac atatgactca attaaatgga
    12001 aatcttttag tttttaaaga ccataaaaga ataatattga gaggaaaaat agattctctt
    12061 gaatcagaaa tactaaaggt acagatactt tgtcagaaac tagatatgcc aaagcttgtt
    12121 aaggaattgc aagaagtatt agactttgta agaaatataa tgagatgtga agttttagat
    12181 gaaaagctag aagactttaa acttttagga ttaactcctc aagaacttag agaacaatct
    12241 cactatccta aaaaatactt tggtatagga catgaattcc caagttatga aatgggagaa
    12301 gtagtagtag ctataaatag tataagaagt aatacacgag aggttgaact tgcagcctat
    12361 gaagcattta agggtgagta tggaaatgtt cacagagaag atttaataaa ggcacttaat
    12421 agattatcgt cagtattttg gataatgata tataaggtta gaactaataa gtataagtag
    12481 ttggaggaca taatggagaa tctattaaat gaaatagtgg aagaagtaat aactagagtt
    12541 aaaaaagaag catttataga agttgaagca tcaggaagac acgttcactt aagtagagaa
    12601 gatgtcgata aattatttgg agaaggatat actcttacaa aactaaaaga cctatctcaa
    12661 ccaggtcaat atgcatgtaa ggaaagagtt acaataactg gtcctaaagg aagtataaaa
    12721 aatgtagttg tacttggacc atgtagaaat gaaactcagg tagaaatatc tttaacagat
    12781 ggttcaactc ttggactaaa agctcctata aaacaaagtg gagacttaga aggtacttta
    12841 agtattaaaa tatcaacaca atatggtgaa gttgaacttg ataaggggtt aatggttgct
    12901 aaaagacata tacacatgac tcctaaagat gctgaaaaat tcaatgtatg tgataaagag
    12961 atagttgaag ccaaggtgat gggtcaaaga cctttaacat ttgatgatgt agtaataaga
    13021 gtgagtgaca gttttaaaac ttatatgcat atagactatg atgaagccaa tgcatgtggt
    13081 tattcaaaag gaactgttgc taaaataata aagaaagcat aaatttaacc tactatataa
    13141 attacctcta gggaggattt aatatgaatt atgataacct tgtagattta ataacggaag
    13201 aaatctataa aaaaataaat agtaatgaaa taaaaatatc taataaacct aaagctgtga
    13261 ttgtatttga acaagataac aataaattta acttattaaa agatgaattt gaaatactag
    13321 aatttgataa aagtataagg gaatgtgaaa tagtgatagt ttcaagactt tgtatgaggg
    13381 ggctatcgaa tatagcactt ggaaattcaa caagtgatga agaaagattt atactaaaaa
    13441 tgattatgaa aggcaaaaaa gtatatgtcc tagatgaagg aattgaatac aaaaaatata
    13501 aggatactgc accaaaagca ttatataaga aatttatgtc atttgaagat gaaatatgta
    13561 aatttggtgt agaaattata aaagacttaa atgttataac taaaaataag ttaaatataa
    13621 agaaagaact agaaagtact agaggttcta aaaaagaaga tacaaaagtt ttagataaag
    13681 atgttttaaa tttaaccagt gtagaaagtg taaaaataga taatgaattt agtttggatt
    13741 taaggaataa aaaacttata tcggaagctg acttaagaaa accaactata aatggagtta
    13801 aaaacatatt agttaataaa aaaagtataa taacaccatt agctgtagat tttatgagaa
    13861 tacaccatct caaactaaag aaactgtaag gaggtaattt tatggatata ggaaaagttg
    13921 taggtaatgt ttgggctaca agaaaagatg aaaaattatg tggccaaaaa cttttagttg
    13981 taaaaatttt aaaaactaaa gacacataca aggaaggctt catggttgca gccgataatg
    14041 caggagcagg aaatggagac ttagttctta tatccaaagg ttcctcagct agagagtcga
    14101 ttgaaaaatc tcatgttcca gtagatgcaa ctatagttgg agtagtcgat tcgtttgagg
    14161 tgtttgatga ataaaagtat aggagctata gagtttaaaa gcattgctaa aggtatagaa
    14221 gtatccaatg aaatgataaa gaaatcttca gttgatgtat tgtacttaaa aagtatttgc
    14281 ccaggtaaat ttctaataat agtaggtgga gaaacttctt atataaatga atgtgtagat
    14341 tatggaataa aacttggaga aggatatatt gtagataact tcgtaataaa tgcaatttca
    14401 caagaaatat tagatggatt taaaaataag tatcaaaagc tagatagtat agtctctata
    14461 ggcgttgttg aaagtagtaa agtatgcgct ggaataaaaa tgcttgataa gactttaaaa
    14521 tctggcgact tagtattagt aaaacttcaa ttatcttttg ctataggtgg caaattagta
    14581 tatgtagtag caggagattt aagtagttta gaatatgcat taaaagaaag tgaaaatgta
    14641 gtaagagaaa aagaagtaat atataagact gtaataccat cagtagacag tcaaataata
    14701 aaaagtctaa taaaataatg gggggaatta tgagtataaa cgaaattata atctatgtaa
    14761 tggtagtctt catggtactt ggagctatag ataaatgtat aggtaacaaa tttggtcttg
    14821 gagaacaatt tgaagaaggt ataatggcaa tggggtcact tgcagttgca atggttggtg
    14881 ttatatgttt agcaccagtt cttgctgatg tattaagacc aattgtagta ccagtattta
    14941 atatacttgg agctgaccca gctatgtttg ctggaagttt attagctaat gatatgggtg
    15001 gtgcgccact tgcacttgaa cttgcgaaag accctaattc tgggttattt ggtggattaa
    15061 tagtaggggc tatgatggga cctacaatag tatttataat accagttgcg cttggaataa
    15121 ttgaaaaaga agaccaaaaa ttcttagcaa caggaatact tgcaggtata ataacaatac
    15181 ctattggagc attcgttggt ggattagttg caggattcga ggtaatgatg gttcttagaa
    15241 acttagtgcc aataataata tttgcagtag ttatagcact tggattatta aaattcgaaa
    15301 atgctatgat aaaaggattc acatactttg gaaaaggtgt agtaatagtt ataactttag
    15361 gacttgctgc tgccatagtt gaagctttaa caggaatagt tgtaatacct ggaatgaaac
    15421 caatcagtga aggtatagaa atagttggag atatagcaat agttttagca ggagcattcc
    15481 cattagtgtt tgttataaca aaggtattta ataaaccatt aatggcttta ggaaaagttc
    15541 ttggaatgaa tgatgtatca gcagcaggcc ttgttgcaag tttagctaac tgtataccaa
    15601 tgtttggtat gatgaaagat atggacaata gaggaaaaat aataaatgta gcattctctg
    15661 tatcagcagc attcgtattt ggagaccact taggatttac ggcaggattt aattctaata
    15721 tgataacacc aatgatagtt gctaaattag ttggtggtat aacagcagta ttgttagcta
    15781 tggttatcgc aaataagaca ctaaggaagg agaatgtata agatggatat atcaaatata
    15841 gataaaaatt taattgaaac attagtaaga caaataatag aagaaaaaat aagtggaact
    15901 aaagacacag tagattttgt tagaaataaa gacattagtg gaataacttc tataaagtta
    15961 ccaactgtaa aagttagtga atctgataga ttagatacag ggaatccaag tgatgtagtt
    16021 tacacaaaag atttatttac attagaggaa agcccaagat taggttgtgg tatgatggaa
    16081 atgaaagaaa caacttttga ttggactcta aactatgatg aaatagatta tgtaatagat
    16141 ggaactttag atataataat agatggaaga aaggtttctg cttcttcagg tgaattaata
    16201 ttcataccaa aaggaagtaa gatacaattt tctgtacctg attatgctag atttatatat
    16261 gtaacatatc cggcagattg ggcatctcaa aattaataaa aatcaagtta agttaataat
    16321 aatcaaataa ataatttaaa atacaaactc ttaaataaac acacatattt aaaaaagcta
    16381 tgaattaaaa ttcatagctt tttttataaa gaatgttata ttttagacgt attttttcta
    16441 tatttactgg gagacatatt atatttttta ttaaataatc taataaaatg ctctacattt
    16501 ggataaccaa tactatttta ctgataatct tttccttatc actaa
//
LOCUS       DAEPLF010000069        15919 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEPLF010000069 DAEPLF010000000
VERSION     DAEPLF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15919
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.069"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            325..1845
                     /locus_tag="KR314_001909"
     CDS             325..1845
                     /locus_tag="KR314_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0328294.1"
                     /translation="MKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIII
                     SIIGILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIN
                     KDKKFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVA
                     LRCYVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINR
                     ANFAQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHS
                     QKINCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENK
                     PFVQISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIH
                     DLHENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHM
                     LKELNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEEILDKEFVYNS"
     gene            complement(2137..2736)
                     /locus_tag="KR314_001910"
     CDS             complement(2137..2736)
                     /locus_tag="KR314_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0328295.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2839..3636)
                     /locus_tag="KR314_001911"
     CDS             complement(2839..3636)
                     /locus_tag="KR314_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH0328296.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3749..3854)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3946..4932)
                     /locus_tag="KR314_001912"
     CDS             complement(3946..4932)
                     /locus_tag="KR314_001912"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBH0328297.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLNEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5416..7146
                     /locus_tag="KR314_001913"
     CDS             5416..7146
                     /locus_tag="KR314_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBH0328298.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7256..7336
                     /locus_tag="KR314_001914"
     tRNA            7256..7336
                     /locus_tag="KR314_001914"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7290..7292,aa:Leu,seq:tag)
     gene            7568..7741
                     /locus_tag="KR314_001915"
                     /pseudo
     CDS             7568..7741
                     /locus_tag="KR314_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
     regulatory      8233..8337
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            8547..11021
                     /gene="gcvPA"
                     /locus_tag="KR314_001916"
     CDS             8547..11021
                     /gene="gcvPA"
                     /locus_tag="KR314_001916"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBH0328299.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHHETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11021..12478
                     /gene="gcvPB"
                     /locus_tag="KR314_001917"
     CDS             11021..12478
                     /gene="gcvPB"
                     /locus_tag="KR314_001917"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBH0328300.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPLIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            12901..15258
                     /locus_tag="KR314_001918"
     CDS             12901..15258
                     /locus_tag="KR314_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBH0328301.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKDDFLYSGSFVVSGEAFVRVENIGKDNYANKIVNDAKISKKHNSQLRNSLN
                     SILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKIRIEVEKYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDNDIYEAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(15344..15661)
                     /locus_tag="KR314_001919"
     CDS             complement(15344..15661)
                     /locus_tag="KR314_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBH0328302.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         6007 a         1864 c         2604 g         5444 t
ORIGIN      
        1 aggagaatta tcttagtttt atttattttg ttgattttta taagttttat ttctattaaa
       61 ttaattcata atgttggaga ttatggaaaa ttgattaatt atgttgggat tgtttgtgga
      121 gctagccaac gtctaactaa actagagatg aatcatcaac caaatgatga attaatagaa
      181 tatattgatg aaatcctaca ggagttgatt acagggcatg gagattatgg tttagtaatc
      241 actgattgta atgaatacaa tgaggactta cttttattag aaaaaaagtg ggaagattta
      301 aatattgaaa ttaaaaaagt aagaatgaaa gaacagaata accagttatt aagtataagt
      361 gaagagtttt tttctcttgc aaatgataca gtgtttaaaa tagaaaactt ttctaaagaa
      421 aaaagtaatt acttaatgac tttaataata attatatcta ttataggtat attagcatgt
      481 atcatattga ttttacaata tagtaaaaaa atggttagac tggaaaaatt aaatgtagat
      541 ttaaaaaata ttgcatataa agatgaacta acaggagtaa atactataga aaaatttaaa
      601 ttggatgcaa atcaaaatat ttgtataaat aaggataaga aatttgctgt attctatatt
      661 gattttgaaa actttaaata cattaatgat atatttggat atgattatgg tgacatgata
      721 ctgaaaagat atgctaatct tatgatgaat gacataggga aatatgaaat atttgcaaga
      781 gaaattgcag atagatttgt tgcgttaaga tgctatgtag ataaggaaga tttagtagta
      841 agacaacgag atgtagacaa tgaactaatt aatataacaa atgaaattaa aaataaacat
      901 agtattactg ttgtcagtgg aatttgttgc attgaagatg taaatgaaaa attaagtata
      961 gatggtttaa taaatagagc aaattttgca caaaaaactg ttaagaataa accaggtact
     1021 aattatgctt tttataatga cagtattcgt aaaaaaatga ttgaagaaaa tactatcaag
     1081 agtagaatac atgaagctat tgaaaaaaga gaatttatag tttatttaca gcctaaagtt
     1141 aatttacata gtcaaaaaat aaattgtgca gaagctttag ttcgttggct tactccggat
     1201 aaaggtatta tatctccagc aatatttatt ccagtacttg agaagaattt ttttattgcc
     1261 ttagtagata agtatgtttt tgaggaggtt tgtaaatgga ttagaaagag gttagatgaa
     1321 aataaacctt ttgttcagat atcagttaat gtttcaagaa tacaatttta taacactaaa
     1381 tttgtagaaa cttattccaa tatacaaaat aaatacagga tacctaaaaa tactatagaa
     1441 attgaattta cagagagtgt tgcttttgag aatcaaaaac atcttttgga aattatacat
     1501 gatttgcatg aaaatggatt tacttgttct ttagatgatt ttggaaaagg ttattcgtct
     1561 ttaagtgttt taaaagattt accatttgat gttttaaagc ttgactctat gtttttcaag
     1621 gctagtttag ataaagataa agagaaaata gtaataaaaa atattgtaca tatgttaaaa
     1681 gagcttaata tcacaactgt tgctgagggc attgaatgca aggaacaagt tgaatttctt
     1741 agggatattg ggtgtgattt agttcaggga tttgtatttt ataaaccaat gccaatatta
     1801 gaatttgaag aaatattgga taaagagttt gtttataata gctaaaatgt actttataaa
     1861 atatgaacta attaagtaaa attattttgt actgaataaa tttttacttt atttacagaa
     1921 tagtgattat ttacagaaaa gggcgaaaag tgtattcctt taagtatggg atggatagca
     1981 caaatagggg agaaaaaata ttggccatat aagaatgcat aataatataa atatttgaaa
     2041 ggtcagcatt atataaaaat taaaaagaaa ataaataatt tttctataaa aaagagacta
     2101 tgagaaaagt tactcatagt ctcctaaata atgtatttat tttcttcctg caacccattt
     2161 taagccccaa tgaaattcag tatccatttc tctatgacct ttgaagtatt taactacttt
     2221 ttcaacactg aatgtctcat catttacatt ttctatcaat gctattgaac acatattata
     2281 gccattttta tgttcatcaa gtttgacaac taagtctgaa tcttgttttt gtttgattgt
     2341 tacaattcca tcagcttcag accagttagc tacaccttcg tatataaaag cataaataag
     2401 tattcttttt atatctttta ttttattacc atttattctt aaattttctc cttgtgtatt
     2461 acttcctgtt ctgtcatctc catctaattg agcaaaaggt ggattgtcta aactaccaaa
     2521 agcatttcct aatgcttgaa cagcaccttt tacaccattt ttcatctcaa ataggcaacc
     2581 caaatctaaa tctatattac tgcttcttaa agagccaaaa aaaccctttt tttgtacttt
     2641 ttgattccaa tttaaattaa ctaagatttc acctaaatcg ctattttcct tttttgtaag
     2701 gtttattttt tgtccctttt ttaattctat agccatagta atcttcctcc acaaacatag
     2761 atttatttaa atggtagcac ttaataagtc aatattcaac aagattagaa taataatatt
     2821 aaaactatac taaatatact aaaaagtagg aatcacaata cctttgtatt tttcttcaat
     2881 taagcttctt gctttatctg atgtaagaac atcttgtaga actttaatct tttcactgtc
     2941 tttatcttca gttctaaatg ctaaaagatt tgcatactcg tcatttatag gagctgtata
     3001 aattccgttt ttcttggcat caagaccagc aggtacagca taagtagtat ctataaatgc
     3061 agcagtaaca tctggaagag aagatggaat ttgagcaata tctaactctt tgaattgtat
     3121 tccctttgga ttttctatta tgtccttagg actaactaat ccatcccctt taggtaacgt
     3181 taaaagtcca atactttcaa ggtatctaag gttcttagag caagaagatg ggttattgct
     3241 tattgcaatt gtatctcctt ttttaaattc atcaacagat tttatcttat aagagtaaag
     3301 tattgctggg catacatata gttttttacc agccattatt ttgtaacctt tggccttaac
     3361 agcttctttt agataaggtt catgttgaaa tagatttgca tcaatttcac cattatttaa
     3421 agcttcgttt ggaagtatgt aatcatcaaa gtttttaact tctaaagtat atcctttttc
     3481 tttaataagt ggttttaatt cttctaataa ctctcctcct ggaactaaag tagcaccaac
     3541 aacaattttt ttatcatctt tagctttaga acatccaacc acagctaaag ttagtactag
     3601 acataataac aaacataata attttttgaa tttcatataa attccctcct gaatataatt
     3661 gattagttaa aaataaaata ccattcttta tcacactgca aaataaaaat aagcctgagt
     3721 ttaaactcag gctttaaaag ctaatttaga ctcatctttc agtataatac tgcaggattt
     3781 agcaccaatg tatataaaaa tatactggtt gctgggtttc atagggccag tcccttcacc
     3841 actctggata agaagtatca atttttaatt atgttataaa tattatcatg attttttacg
     3901 tctgtaaaca taattttata aatattttaa aatctttaat gtattttatt taacacctaa
     3961 aattccagaa actagaatat cagtatcagc acctaagaaa tagtcattta tttcaataga
     4021 atccagagta tttctcaaag cattttcttc atgccttata ccaataagtt tattttctac
     4081 aaatgcaaca tcacattttc caaaaaaatc tccaaaaaat tttatactag ttattatgcc
     4141 tttatcaaca tttaaattaa actcaacatt tccaccagaa tattttaatt catttgaaag
     4201 agtatatttt ggtgaattac caaaattcca atcccaagtt tcgtattttg atttagtaag
     4261 cttttctatt tgttttatat catgctcatt caaacaataa ttagtattat cagtatttgt
     4321 tttagaaaga taatccatta tagcatcttt aaattctaat atgtccattt ttgaatgtaa
     4381 atgttcactt atattggtta cacgagattt tacagatttt attccttttc cttcaaattt
     4441 tataggttta acctttaatg cttttgagag gtcatttatt tcagatgaaa ataaaagagt
     4501 accatgatgc attactttgt ttttgtagtt gtattgagca tttccagaaa atttcttgcc
     4561 atctataaga aggtcatttc ttccagaaaa ttcggcattg acatctagtg ttttaagtaa
     4621 atctattatg ggctgagtga atcttctgaa atcactaaaa ttattattgt tacaagctat
     4681 aaatgtaaaa tttatatttc ccaaatcatg aaatacagct ccaccaccag actgtctcct
     4741 aacgacaggt atagaatatt cttttatata ttctaaattt atttcagaaa gtgtgttttg
     4801 gttttttcca acaattatag aaggttcatt cctccataaa ataaataagt cttcatcaaa
     4861 attttttaga agatattctt ccattgctaa attaaaataa gggtttgttt tttcattata
     4921 aattaataac attaaagtag ttcctccata gaattgataa aatttttatt atgaatacaa
     4981 ttaattttat aattcgacaa taatataaaa actcctttaa attagaaatt ttaaaaacta
     5041 ccttgcaaag tttaaataaa taatatatac tcaaaatatg tttaataaaa gtgaatagaa
     5101 gggtagaggc gcaataatct atcagtacta ataaggagtt aagcacagag agttattagg
     5161 aaaggagata ttgccgaagt tttataatcg atgctttaag attatattgc tgggggtaca
     5221 tagaaaatat gtacaactgt cacaggttta tttgtggtga gctatcattt aaggaattat
     5281 attatattta tttttactat tgttgatata ttgtaatgtt taagccttga gtgtttacca
     5341 ctcaaggctt ttttagtttt attgtaacat aataccctgt acagttcaca aaattaaaag
     5401 gagagggaga tttatatgag gaacaaaaaa attaatatta tttttctgac gacaatcatg
     5461 ttcataatgt caacagtaat ggtttttgca gaagaagata tagacactat tgcactagct
     5521 aatgcagaaa aatttgggat tttaacttta ataccaccat tagttgctat aatacttgca
     5581 tttataacta aaaacgtaat aatttcttta cttataggta ttctttctgg tagttttata
     5641 attaaagcta gtggtattaa tgtttttgcg acatttatac aggcattttt agatttagta
     5701 gatagagctt tagtatcttt agcagaccct tggaatgcag ggataatact acaggttttg
     5761 gcaattggag gagtaataaa tcttgttgct aaaatgggag gagctaaagc tatagctgaa
     5821 gcacttgcta aaagagcaaa gtcagcaaaa gggacacagc ttataacatg gtttttgggg
     5881 cttttagtat tctttgatga ttatgccaat tcactaatag taggtcctat gatgagacct
     5941 gttgcagata aaatgaaaat atcaagagaa aagctagcat ttataataga tgccacagca
     6001 gcaccagttg ctggtttagc tataatatct acatggatag gtcttgaggt tggtttaata
     6061 catgatgctt ttgaaagtat aagtatagat gtagatgcat ttggtatatt tttaaataca
     6121 ataccattta gattttacaa tatattaata ttagctttca ttgtaatatc agcattatta
     6181 ttaaaagaat ttggtccaat gagaaaagct gagataaaat ctagaagtag aaaaattagt
     6241 atagaccttg atgaaggcgt agaagagttg gatgatttag ctccaaaaaa tggagtaaaa
     6301 ttgagtgtat ggaatgctat aatcccaata ggaacattga taatagttgc attagcatca
     6361 ttttattata gtggatatac atctataatg ggaggagacg ataaggctct gatacaacta
     6421 tttacaaatt ctccatattc ttttgaagca ataaaagaag catttagtgc atctgatgca
     6481 tctagagcat tatttcagtc tgcacttgtt gcaagtttag ttgctattat aatggctgta
     6541 gtaaaaaaga tttttacaat atcagaagca attgatgttt ggatagatgg aatgaaaagt
     6601 ttagtaatca ctggtgtaat attaatactt gcatggtcat taagttcagt aataaaagag
     6661 ttaggtacag ctaagttttt aatacaccta ttatcaggtt cactaccacc attcttatta
     6721 ccaagtttaa tatttggttt aggagcaata atatcttttg ctactggaac agcttatgga
     6781 acaatgggta tacttatgcc acttgcaatt ccattagcat actcattaaa tcctgatatg
     6841 tcttatgtaa tagtaagtac tagtgcagtt ttaacaggtg caatatttgg agaccattgt
     6901 tctccaattt cagatacaac aatactttct tcaatgggag ctggctgtaa tcatatagac
     6961 catgttaaca ctcaaatgcc atatgctata tttacagcag taatcactat tgtatttggg
     7021 tatataccag ctggacttgg attgccaata tatatagtat taccagtggc aatagctgct
     7081 atatttgtag gtattcaaat aattggtaag aaagttgatg aagcggaaat agaacttgta
     7141 gaataaagat ataaataagt ttcacctttt attatcacta ttttgttagt ttttagataa
     7201 ttttaaaaag ttattgaaaa taatggcgaa atatagtaaa ataaaagagt gataagcgga
     7261 tgtggcggaa ttggcagacg cactagactt aggatctagc gccattggcg tgggggttcg
     7321 actcccttca tccgcacttt acagtactta attgtactga aaaaaactgg ggttgctggc
     7381 tttcggagtg ggctatcccg tttttatttt tttgtatata taatagattg gcactttagt
     7441 ataaggtgct ttttttatag gcaaaaacag tacatttatt tctgttttat tatcattcat
     7501 ctattagaat atactttatt taagaatgtg ttaataagca gtgttcacat tcttgataaa
     7561 atgtggaata atatgcctac caataagcat ttggtgtata agtaagagta tttatatagt
     7621 tgattgtgaa atgagtacaa caattctgta cagtataatt ggcattataa ttgtagcatt
     7681 gaatttaaag tttgcacagt cgctgattgg aaaatttaca aagtggatgt caaacatata
     7741 aattttagtt tattggacag ttcaaggaat cagaaattgg aattttatag tattaatatt
     7801 ttatagtatt aatataatgt ataaaatttt atagcaatgt atttcataag tataaagtga
     7861 ctagaatttt aagaaatata caaattctat aaaaactaaa taatttcaag tatattttaa
     7921 aaatcttatt tttcacaagt ttaaaattgt aaacgatttc atcaagtcta tgaatatact
     7981 tttaacatgt tatagtaaat ctcaatataa atttacattt ggttctttgt gtgttatttt
     8041 aataataaat taagtgaaaa tgttatttta cattaatata taatatcttt gcataagagt
     8101 gaataatata aaagagaaag aattgataat aaaaacttaa caaaaaaatt aatacataaa
     8161 aaaaaataaa tataatgtgt tgacatttaa ttgaaatatt catacaataa aattgaaaat
     8221 aaatatttcc aaatgagatt agcgagagag tcctttatac gaaaaggcgc cgaagaagaa
     8281 atttaagaaa tgccaatttt tagagaagct ttcaggcaaa aatatcgcta attgatggga
     8341 ctctggagag acttaagtta agatactata agtcaccgaa gaagcaaaat aatattaaat
     8401 tattaaatct ttcaggtata caaacaggga tatataggct aggattattt tccatgctga
     8461 tatatctttt ttttatgaaa aagtacagga aataatatca aagttactta aatacagtag
     8521 gtctaatttt ctagggaggg ttatatatga atgaattgaa aagagtatct ttgtacaata
     8581 tacacaaaga gcttggagca aaacttgttg aatttgctgg ctgggaaatg cctttagaat
     8641 atgagggtat aaataaagag catgagaagg ttagaaaaag tgcaggtata tttgatgtat
     8701 ctcatatggg agaggtacag ataaagggag cagagtcaga aaaatttata caaaacctag
     8761 ttacaaatga tataagcaca ttaaagataa atgacattat atatacacct atgtgttatg
     8821 aaaatggtgg agtagttgat gatttattaa tatataaatt tggtgaagaa gattatttat
     8881 tagtaataaa tgcaggtaat atagataaag atgtagcatg gataataaaa caaagtgaag
     8941 gatacaatgt agatataaaa aatatatcta gtgaagtgag tcaattagca attcaaggac
     9001 ctaaagcaga agaaattctt caaaaaatca cagatataga cttaaactca ataaaatttt
     9061 ataagtcaat tccatcaaca aaagtatgtg gttgtccttg tttagtttca agaacaggat
     9121 atactgggga agatgggttt gagatatact gtaaaaataa atatgtagaa ataatttgga
     9181 atgaagttct aaaggttgga ggagaagata tatgtccagc tggtctaggt tgtagggata
     9241 ctttgagatt tgaagcagct ctacctctat atggtcatga aataaatgaa cacatatctc
     9301 caattgaagg tggtttatct atatttgtga aaacaaataa agaatcattt attggtaaat
     9361 caattttatc aaaagaaaaa gaaagtggag ctaaaagaaa gttagtagga tttgaaatgc
     9421 aaggtaaagg aatgcctaga aatggatatg atataaggat tggtgataaa acagttggat
     9481 ttgtaacaac aggatgtgca tctccaacga caggaaaaat tttaggtatg ggaattatag
     9541 attctgaata tgcaaaagta ggaaatgaaa taggtatagc aatcagaaaa aaagttgttc
     9601 ctgctgtgat agttaagaaa cctttttata aaaaacaata taaaaaagat aatattatat
     9661 taaacaaaga aaataaattt tcatatatac ctgctacaag tgaagataaa tcaaaaatgt
     9721 taaaagtagt tggattaaat tcggttgatg aattattttc agatatacca gaagaagtaa
     9781 aattaaaaag agatttaaat ttggaaattg gaaaatctga attagaggta agcaaaatag
     9841 taaaaagatt gtcagaagaa aacttaagtt tagaagactt gacttgtttt cttggtgctg
     9901 gtgcatatga ccattatata ccatcaatta taaaacatat aacttcaaga tctgaatttt
     9961 atacagcata cactccatat caagctgaaa taagtcaggg aactttacaa gtagtttttg
    10021 aatttcaatc tatgattgca gaaattacag gtatggaaat tgcaaatgca tcaatgtatg
    10081 atggagcaac tgctgcaata gaagcatgta taatggcaat gaatcagact agaaaaagta
    10141 aaatagtagt gtcaaaaact attcatcatg aaactttgtc agtattaaga acttacttac
    10201 aatataaaga ttgtgagata gtagaaattg acttttgtaa tgaatatggg actacagata
    10261 tagaaaaatt aaaagcttct gtagacaagg atacagcatg tgttcttata caaactccta
    10321 atttctttgg aattattgaa gaaatggaag aaatagaaaa gataactcat gaaaataagg
    10381 ctatgttaat tatgagtgta gacccaatat cattaggagt tttaaaaaca ccaggtgaga
    10441 taggtgcaga tattgttgtt ggagaagctc aatcacttgg aaaccctctt aattttggag
    10501 gtccttatgt aggattttta gctagcaaat ctaaatatac tagaaaaatg ccaggaagaa
    10561 tagttgggca aagtttagat gtagaaggta aaattgcata tgtattaact ttacaaacta
    10621 gagaacagca tgtaagaaga gaaaaagcaa cttctaatat atgttcaaat caagctttaa
    10681 atgctcttgt agcttcaata tatatggcta ctatgggtaa agaaggattt aaagaggttg
    10741 gtatgcagtc tatgaaaaaa gcacattata cttacaataa gcttgtacaa actggaaaat
    10801 ataagccaat atttaaaggt aagtttttca aagaatttgc tgtacaagga aatcttaata
    10861 tagaaactat aaatgataag ctcttagaag aaaatatatt aggtggttat aatttagaat
    10921 acaattatcc agaattaaaa aattcaactt tgctttgtgt gactgagaaa agaagcaaag
    10981 aggaaataga taagttagtt ggaataatgg agggattata atgaaagaat acaatagttt
    11041 gttgatagat atatctaaaa aaggtaggaa agcatactca cttccaaagt tagatataga
    11101 tgatataaaa atagaagata tgattgacca agatatggca agacaaagtg agttgaattt
    11161 gccagaagtt ggtgaattag aattagttag acattataca ttattatcaa ataaaaactt
    11221 tggagttgat acaggttttt atcctttggg ttcttgtact atgaaatata atcctaaaat
    11281 aaatgaagat atggcagcac ttccaaactt tactggaatg catccatacc aatcttcgga
    11341 tacagctcaa ggttctcttt ctttgatgta tgatttatca agaagacttg cagaaattac
    11401 aggtatggat gaagttacac tacagccttc agcaggttct catggtgagt ttacagggct
    11461 tatgattata aaagcatatc atgaaaatag aggtgaccat aagagaacta aagtgataat
    11521 tccagattct gcacatggaa caaatccagc aagtgcagct atggcaaact ttgatgtgat
    11581 tcaaatagca tctgataaaa atggagctgt agacataaat gcattaaaag aggttttgaa
    11641 tgatgaagta gcagctctta tgcttactaa tccaagtact cttggtttat ttgaaaaaaa
    11701 tataaaagaa atagctactc ttgtacatga agcaggagga cttttgtact atgatggtgc
    11761 aaacatgaat gctattatgg ggataacaag acctggagat atggggtttg atgtggttca
    11821 tcttaatctt cataagactt tttcaactcc tcatggaggt ggaggtccag gagctggtcc
    11881 tgttggagtt aaaaaagaat tagtaccttt tttaccaata ccattaattg aaagaaaaga
    11941 agataagtat gtactaaatt atgatagaga aaaatctatt gggaaaatta aaaatttcta
    12001 tggtaatttt ggagttcttg taagagctta cacatatata ctaactatgg gaagggatgg
    12061 acttaaagag gctagtgaaa tggcagtttt aaatgctaat tatataaagg aaagtataaa
    12121 agatgattat attttgccaa tagatacctt gtgtaagcat gagtttgtat taggtggatt
    12181 gccaagagga gaggccaata taaaaactat cgatattgca aaaagactac tagattatgg
    12241 ataccatcct ccaacaatgt actttcctct tattataaat gaggcactta tgatagaacc
    12301 tacagaaagt gagagtatag agacattaga tagttttata gaagctatga aaagtgttgc
    12361 taaagaggca aaggaagaac cagaactatt gaaaactgct ccacataata ctttggtcaa
    12421 aagagtagat gatgcaaggg cagtaaagaa acctatacta acatggtcac aaagataaat
    12481 aattaaaaag tagagattta aagtataaag agaattaaat ttgtaagaaa aagctatcta
    12541 acttaatcca gtttagatag cttttttgta tattagtatg tatggtattt tctatataaa
    12601 gttttaaatt atattagtgc ttatatagaa tattgatatg atgattatgt atagatatta
    12661 ataagaattg ttgaatttat cttgccttaa ctttatttac tcttttatct taagaaaaac
    12721 ttaactaaaa tatagcgaaa ttcataaaaa tttaggaaat cttaagaatt ataatattta
    12781 aatctttaga tttatatttt attatataaa ctatagaata ataaaatata aaaattgaga
    12841 attttataat atatttgtag atttgagtat aaaaaagtgc taagttgaaa ggagtaaaat
    12901 atggataagg ttaatttaga aaggtatagt tgtgatataa atgttggttt aaataaaaat
    12961 caacttcaaa gtagaataga tgataattta attaatgcat ttgataatgg aaaaaccaaa
    13021 acatataaac aaatttttaa agacaatata tttacacttt ttaatctcat aaatattgtt
    13081 ttagtctgtt tattagtttc agttggttct tttaaaaata ccttatttat atttattgct
    13141 gtaattaata ctattatagg agtaatacaa gagataagag ctaaaagatt attggacaat
    13201 ctatctgtta tagtttcaaa taaggtatct gtcataagag aaaaagagaa aaaagagatt
    13261 gatgtacaag aattagtatt agatgatatt atgattttaa aaacaggtaa tcaaatttgt
    13321 gcagattcaa aagttttaaa tggaaaatta gaggtaaatg agtctttggt tacaggtgaa
    13381 tctgatatta ttataaaaaa taaagacgat tttttatatt ctggaagttt tgtagtttct
    13441 ggagaagctt ttgtaagagt tgaaaatata ggaaaagata actatgcaaa taagatagtt
    13501 aatgatgcaa agatttctaa aaaacataat tctcaactta gaaattcatt aaattcaata
    13561 ttaaaaattg taggtataat aataattcct ttaggaatta tattgtttgc aaaacagcat
    13621 tttggaaatg gagattctat agctacatct gtagtaggta cagtggcagc tgtaaatggt
    13681 atgataccag agggattaac attacttacg agcattgctc tagctgtagg aactataaaa
    13741 cttgctagtc ataaaacttt agtgcaagaa ctgtactgtg tagagactct tgctagagtt
    13801 gatacactat gtcttgataa gacaggtaca attacagaag gtaaaatgca agttgatgat
    13861 atagtaatgt tggaagaagt ggatattaat gaaataatgg ggaatatatg taattctctc
    13921 aaagatgata atgcaacact aaatgcaata agagataagt ataatgtttt agatacgtat
    13981 aaagctaaga atttaatacc attttcttca gaaagaaaat acagtggggt tacctttgaa
    14041 aataaaggta catatttatt aggtgcattt gagtttatat ttaaggaaaa aaataaaaaa
    14101 attagaatag aagttgaaaa atattctaaa aaaggaaaca gagtaatagt tttagctcat
    14161 agtgattctt atatggaaga taataatatt cctgaagata taaagccact agcttttata
    14221 ttgatattag ataaaattag agatgaagct aaggaaactt tagaattttt ttacaatgaa
    14281 ggtgtagata taaaaattat atctggtgac aatcctataa cagtaagtga ggtagctaga
    14341 aaagcaggtc ttaaaacggc tagtaatttt attgatgcaa caacacttaa aaatgataac
    14401 gatatatacg aagcagttga taaatacagt gtttttggaa gggtaagccc acaacagaaa
    14461 aaacaaatga ttttagcatt aaaaaaacaa aatcatacag tagctatgac tggagatggt
    14521 gtaaatgatg tgctagcgct taaagaagca gattgcagca ttgccatggc atctggaagt
    14581 gatgttacta agaatgtttc taatttagtc ttattagatt caaactttgc atcaatgcca
    14641 aaggttgtta tggaaggacg aaaggtaatt aataacattc aaagagcatc ttctttgttt
    14701 ttagtaaaga caattttttc attcttcttg tcactattaa cattattctt cttcagtaga
    14761 cagtatccat ttgttcctat acaattatca ctaattggag ctgtaacagt gggtatacca
    14821 tcattcttct tagctcttga agcaaacaaa agtagaattt caggaaactt tttactcaat
    14881 atattaaaaa atgctttacc aggagcacta tgtgtgacag taaatgtaat tattgtttac
    14941 aatatagttg aatatttagg ctatagttca agtgtatttt ctacaatgtg tgctatatta
    15001 actggtgtat gtggtctttt aatattaaga caacaatgtt tgccatttaa taaagaaaga
    15061 ttatttttaa ttgtaagtat gactattgca tttgttattg ggattctatt tttaggtgga
    15121 ttattctcat ttgtagcatt gagtagagaa ttaattctca taactatggc attagcaata
    15181 ttaatgccaa ttatgataaa agttatgtta tttgtcttgg atgagatatt agagacaatt
    15241 gttccagatt taaattaatt ttaaaatgat ataaataaga gcagttttag ttgaactgct
    15301 ctttttagat gtgtttaatt ttatataaga atatgtattt ttattaatca gctatatatg
    15361 agcaacagta gtcaacaact tcattaacct ttaaatcaaa acttgaatca ctaggaacat
    15421 taaatttttg cccttcttta taagttacaa actcattgct tccaggcagt tttacatcca
    15481 tacttccagc aagcatctcc attatttctt cttctctagt agaaaaagta tattctcctg
    15541 gcaacattaa tcctaaagtt tttctttctc cattaggcag tattattact ctgctactta
    15601 ccttaccatc aaaataaacg tttgccttct ttactgcagt aacattctta aattcactca
    15661 ttagataaaa cctccaaaat tatttgtaca tatatattat ataaaaataa tgtaaaaaat
    15721 gctatatatt tgcaaaaaaa atttagtatt tattaaaaaa attaataaga ataaaaaaga
    15781 gatgaaattg cttaaattga taaaaaatat aaaataaatt gcaaagtaag caagaaaaga
    15841 ataagttgaa gagttatata ttataaatat taaaaatttt agtattaata aaaaagatat
    15901 tacattaata tgaaaatat
//
LOCUS       DAEPLF010000070        15493 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEPLF010000070 DAEPLF010000000
VERSION     DAEPLF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.070"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            142..1011
                     /gene="galU"
                     /locus_tag="KR314_001920"
     CDS             142..1011
                     /gene="galU"
                     /locus_tag="KR314_001920"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBH0328303.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            1036..2049
                     /gene="galE"
                     /locus_tag="KR314_001921"
     CDS             1036..2049
                     /gene="galE"
                     /locus_tag="KR314_001921"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBH0328304.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            2161..4095
                     /locus_tag="KR314_001922"
     CDS             2161..4095
                     /locus_tag="KR314_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBH0328305.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKAINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLSV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSLEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            4250..5113
                     /locus_tag="KR314_001923"
     CDS             4250..5113
                     /locus_tag="KR314_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBH0328306.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(5321..6202)
                     /locus_tag="KR314_001924"
     CDS             complement(5321..6202)
                     /locus_tag="KR314_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0328307.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            6615..7463
                     /locus_tag="KR314_001925"
     CDS             6615..7463
                     /locus_tag="KR314_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBH0328308.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGTEEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            7542..9464
                     /locus_tag="KR314_001926"
     CDS             7542..9464
                     /locus_tag="KR314_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0328309.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDIHDSSVIAAYLEGCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            9546..10409
                     /gene="aroE"
                     /locus_tag="KR314_001927"
     CDS             9546..10409
                     /gene="aroE"
                     /locus_tag="KR314_001927"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBH0328310.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGIEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNAKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            10609..11826
                     /locus_tag="KR314_001928"
     CDS             10609..11826
                     /locus_tag="KR314_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0328311.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLIVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            12062..13927
                     /locus_tag="KR314_001929"
     CDS             12062..13927
                     /locus_tag="KR314_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBH0328312.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIEYNKIEFEKEINDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(14105..15469)
                     /locus_tag="KR314_001930"
     CDS             complement(14105..15469)
                     /locus_tag="KR314_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0328313.1"
                     /translation="MSQLLIKNAYLITMNADREVYETGSILIENNIIKNVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLIRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHNPGAAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
BASE COUNT         5927 a         1794 c         2775 g         4997 t
ORIGIN      
        1 aaggtagatg aaatttcatc taccttatat tatcttatag cattaaatat tgggataata
       61 tagagtttga agaaaaagta taaatatggt atattaattt ggtacaatag aaaatttacg
      121 aagaagggtg aaaaaatgca gatgacagtt aaaaaagcaa taataccagc agcagggcta
      181 ggaaccagat ttttaccagc aacaaaatca cagcccaaag aaatgcttcc tatagtagat
      241 aaaccaactt tacagtatat aatagaagaa gctatagaat cgggaattga agaaattctt
      301 ataataacag gtagaaataa aaaatctata gaagatcatt ttgataaatc agttgaatta
      361 gagttagaac tagagcaaaa aggtaagaca gagatgttag aaatggtaag agacatatca
      421 aatatggtaa atattcatta tataagacaa aaagagccaa aaggtcttgg acatgcaatt
      481 tactgtgcaa aaagttttat aggtgatgaa ccttttgccg tattattagg tgatgatata
      541 gtagattcag atgttccgtg cttaaaacaa ttaatagata cttataatga gtataaaaca
      601 actgtattag gtgttcaaaa gatagctaaa gaagatacta ataaatatgg tatattggat
      661 gtaaaacata tcgaagatag agtatataaa gttaaagata tggttgaaaa acctgcaatt
      721 gaagaagctc catctgatat agctatactt ggaagatata taataactcc tgcaatattt
      781 agtatattag agaaacagac acctggtaaa ggtggagaga tacaactgac agatgcactt
      841 caaactcttg gaaaacaaga ggctatttat gcttataact ttgaaggtag aagatatgat
      901 gtgggagata agattggttt tttagaggca acaatagact ttgctctaaa gagagaaaat
      961 ttaaaagatg atttaatgga ctatatgaga aaaaaagtaa gtgaaaaata aaatattaga
     1021 aaagaggtaa ttacaatggc agttttagta gctggaggag caggatatat aggaagccat
     1081 acagcaatag aacttttaga atcaggttat gaagttgtta tagttgataa cttaagtaat
     1141 agcaactcaa tagtggttga tagaattaag gaattatcta aaaaaccagt aaaattttat
     1201 aatattgata tcagaaataa agatgaaatg catgttgtat ttaaagaaaa taatattgaa
     1261 tcaattatac actttgcagc tttaaaagca gttggtgaat cagtagaaaa accaattgaa
     1321 tattacagca ataatctaat aagtacctta aatttatttg aattaatgag agaatatggt
     1381 gttaaaaagt ttgtatttag ttcttcagct actgtatatg gagacccaca tacttgtcct
     1441 atattagagg atttcccact ttctgtaacc aatccatatg gaagaactaa acttatgata
     1501 gagcagatgt tagtagatat atcaaaagca gataagtcat tggatatagc tcttttaaga
     1561 tactttaatc cagttggtgc tcataaaagt ggtagaatag gtgaagaacc aaatggagtt
     1621 ccgagcaatc taatgcctta tataacaaaa atagcagtag gtaaattgaa ggaattgagt
     1681 gtttatggta atgattatcc aacacatgat ggaactggag ttagagacta tatacatgta
     1741 ttagatttag ctgctggtca tgtaaaagca ttacaaaaat tagaagaaaa tccaggactt
     1801 gttgtttata atttaggaac tggaaagggt tatagtgttc ttgatttagt aaaagcattt
     1861 tctaaagcaa gtggaaaaga aataccatat aagattgtag gaagaagggc tggagatgta
     1921 gccatgtgtt atgcagattc ttcaaaggca gaaaaagagc ttggatggaa agctaagtat
     1981 gaacttgaag agatgtgtga agattcatgg agatggcaaa gtatgaatcc taatgggtat
     2041 gaagagtaga atacatactt atatgtataa atgtaacaaa tttgtaaata taaggtattg
     2101 gtaattttat gttataatta gacacatgta gtaatgtttc taaaagaggg ggaagttata
     2161 gtgaaaaaaa gtctaaaaaa gtatttagta ctagcactga ctttagtttt agtatttgca
     2221 tgtggcgtac cagaatcaag cgcagccagt aaacatgtga ttatagtaaa ttcaagaaag
     2281 aatactctag gatattttgt aaataataaa cttgtaaaag aatttagagt tgcaacaggt
     2341 aaaaaggggt cagaaacacc aacagggaaa acaaaagtag ttaacaagat aaaaaataga
     2401 ccatattata aaggtaatat acctggagga agtccaagaa atccattagg agatagatgg
     2461 atgggattag cattaaaagg aacttatgga gatacatatg gaatacatgg aaataataat
     2521 gagagttcta taggaaagca tatttctggt ggatgtataa gaatgcacaa taaagatgtt
     2581 agatggttat ttgaccaagt tcctgtaggt tcagatgtaa ttatagatta tagtaatgac
     2641 agctatgtaa agatagcggc aaaatataag ataaatctaa atcaaacagg atggaaaaca
     2701 gaaaatggta agaagtatta tgtaaaatct gatggaacat accaaaagaa tagttggcta
     2761 aaagtaaatg gaaaaatgta ttactttgat gcttcaggag taatgcaaac aggatggaaa
     2821 gcaattaata acaaaaaata ttatttagga actgatggag caagagtatc tggatggaaa
     2881 gtaatagacg gaaaaactta ttactttaac tcagatggag taatgcaaac aggatggcaa
     2941 gaaaagaatg gaaagaaata ctatctagga tctgatggtt tatcagtaac aggatggcaa
     3001 gaaatagatg gtaataagta ttactttgac aaaacaggaa taatgcagac aggatggcaa
     3061 caaatagatg gtaagtctta ctatttagac aaagatggaa agatgctaac tggttctcaa
     3121 aaaatagatg ggaaagatta tacttttaat gaagatggaa ctataaatcc tacatgggat
     3181 acaattatag gtgcaaatag atttgataca gctaaaaaga taagttcagt tggaaactgg
     3241 aatgctgata gttcagatac tgtaatatta gtaaatggta atgcaatagc tgatggtata
     3301 acagcaactc ctttagcaag tagctatgac tcaacaatac tacttactaa tacagctaat
     3361 ttaccaactg aaactgttga agaaatgaag ttattagcac ctaaaacagt aatcttgata
     3421 ggtggagaaa atgcaataag ttctaaatta gagcaagaaa taaaaactac tttcaacaca
     3481 gagacaaaga gaatagcagg tcaagataga tatcaaactg ctacaagaat agctgaagaa
     3541 cttggaaatc gagaagaaat aaaaactgca tatatggttt ctggaaatgg ggaagcagat
     3601 gctttatctg tagcttctaa agctggggaa gaaaaacaac ctataatatt agtcaataaa
     3661 gatggaatta cagaagaatc ttataaatgg ctaacagaaa gaaaattaga aaatgcatac
     3721 tttataggtg gaccaagtgc aattaacgat tcagtaatag caaaaatgaa tgatataact
     3781 actgaagata tttcaggaaa tagaatatat ggagatagta gagtagatac taatgccaaa
     3841 gtaatagaaa aattctatgg agatactgat ttacaggctg tattagttag taaatcagat
     3901 gctttagtag atgctttatc agctggacca ttagctgtaa aattacattc accaatagtt
     3961 ttaatggata attcaggatt atcattagag caacaaagag tatttgcaaa taaaaaagtt
     4021 gaaactcctt atcaaatagg aggcggagta tcttatatag taatggataa attaatggat
     4081 attcttgcaa aatagtaaaa atatatattg aaaaataatt ataattgaga tgtgaaattc
     4141 aatcaataaa agcttgattg ttttacatct caatttttta gtataaaatt aagtttaagt
     4201 tatataaaaa tcttggaata tttttaaaac tcatataaaa tgaatataga tgctatcatc
     4261 atatgttaag agggtgagta agattataaa gaaaaaatat ataatgttta ttgatgaaag
     4321 tggaagagct gatataaatc atgaagaccc atttacattg acaggagtaa tttttgaata
     4381 taaatatgca gtaagtcaag gcgattatgt atgttctttg aggaaagaaa tggatgcatt
     4441 taaagagtat tgttttggaa ccagtaacat aggaatacat ttgacagata tatcaagaaa
     4501 atcaagagca tttgagaagt ttgatgattc acagattaag ttattctatg atgagttgcc
     4561 tttatttcta tctagattag agtgtactat aatatcagta acagtagata aagctagatt
     4621 aaagaaatat tatgcacctt caaaggaacc gtatgttgtg gcttttatac atgttcttca
     4681 gaatttttat tcatttgtaa ataatgaaac tgtagaatct gcacgaatag ttatagagtc
     4741 aagggatgat gtctcaaact taaaagtaca aaaggcgttt tttgatgtat ttaataatgg
     4801 aacaatatac ttagatatag atgaagaatt aagagataag gttaagggtt ttataattgc
     4861 caaaaaagaa gatgctgact atagagaagg tttagaaata gcagatattt tatgtaatcc
     4921 tcttagcaga gcaagaagag ggttaataga agctaatcca aagtgtatgc aatatggaga
     4981 aaaaaacaaa atattcaaat ctgtcagaaa caaaatatat actccaacga gtgtgaatga
     5041 tataagaaat tggggattta aagaagttcc tattgtagag cctgttggac catggcttga
     5101 aaaaatgcgt tagatacaat tgacaaattt aaaaaaatct gatataatgt aaataaccta
     5161 gttattcagg aactgaatcc agttgctgtt gtataacgat aattactgag actcggatgc
     5221 cgactgtcag ttaggaatct atacttttaa aagtatagat ttttttattt tataaaaaga
     5281 ataaaagagg atatttaaaa ataacccctt tattaataaa ttataattga gcgtttttaa
     5341 tgacaaagtt tttaaagttt ttaattgcag gaggcaaata tctatctttt ataaatgcta
     5401 agtaaaagtt tctttcccaa cttgggtgaa taatctgaat tacttttact tttgtaaagc
     5461 taagcatatt catatttggg actacagcaa ttccgaaatt ttttgcaact aatccagcaa
     5521 ttacttggtc ttcctctact tcatagacta tattaggctg ttgatttatt tttttaaaca
     5581 tatcatcaat tatatatcta agaccacttc tctgattaaa tactatttga ggataaggta
     5641 tggtttcttt taaatctata gtatcctttg cagctagtgg atgactatat ggtacaatta
     5701 gtactaaatc ttgtttggca acaggtataa attcaattcc cttttctttt tcaagtttcg
     5761 aacaaaatgc aatatcatat tttctttctt taagaccttg aataatgtca gtagttacac
     5821 cagtatgaaa tttaaaatct attgatttag caggattaga ttttaaaaat ttatgcacta
     5881 tgtctgggat aaaatcagta ccaagtgttc taagaaaagc taagtctatt tgtccttcac
     5941 cagtaccagt tatttttaat gattttacac ttgaatctaa aatttctaat gatttttcca
     6001 catcttttaa aaatattttt ccatacttag taaggactat atttctacct tctttttcaa
     6061 atagagccac cccaagttca ttttctagta aagaaatagc atgacttaag cttggttgtg
     6121 ttatagataa attttctgcc gcttttgtat agtgttctaa atgtgccaat gtaacaaaat
     6181 agcgtaagtg atataaattc aatttttgtt catcctttca taggtatttt tatttatata
     6241 tttgtagaag ttaaccttta agatattttt acatttttat tatatatgat tatagagtaa
     6301 gaataaaagc ttttaaaaaa atttatcgtt ttttgtctga attataagat aaatatattt
     6361 atacaaccta atagaaaaat acaagaatta tcttataaat aaaatctata aacaaaatct
     6421 atgaattaat aggaaattat caatttgtta ttagtaaagt ctcgtgatac aataaattta
     6481 taaaatctag ataaatagaa atcaaaaaat acttttattt ttaaggtaaa ataatactac
     6541 tagtaggata aataaaaata taaaatcaaa gatactatta attataaaga aaataaaggg
     6601 ggcaaattta agctatgaat aatcaaaaaa atcttaatat tccaattaca attagttctt
     6661 atacattggg aacagaagtt tcattccgtg atagagttcg tattgcaaaa gaagctggat
     6721 ttgatggtat tggattgcgt gctgaaaatt atattgatgc aaaaaatgca ggtgttacag
     6781 atgaagaaat gattgcaatt cttaatgaat atgatatgaa agttacagaa gtagaatata
     6841 taactcaatg gggaacagaa gaagatagaa ctgaagctca aagagaaaaa gagcaaaatg
     6901 tttatcatat ggctcactta tttaatgtta aacatataaa ttgtggatta ttagagatga
     6961 taccagaaga acaaattata actgcattgg gagaattatg tgacagagca ggtgaactta
     7021 ttataggtct tgagtttatg ccatatagtg gtgtaccaga tttggctact gcatggcgtg
     7081 ttgtgaaagg atgtaatcgt gaaaatgcaa tgcttatatt agatacttgg cactgggcta
     7141 gagcaaagca gacagcagag gaccttgcac ctgttccagc agataagata gtatcaattc
     7201 aagtgtgtga tgttttagaa actccttatg aaaaacttag ggatgaatca ctacatgacc
     7261 gtttggctcc aggtgaagga tatggaaaca cagaggaatt tgtgaaaata ataaaagaac
     7321 acaatatatc accaagagtc ataggggcag aagtaatatc tgattctcta atatctaaag
     7381 ggttatccac tgctgctgaa actgtattta atgcaactaa aaaagttttg gataaagcat
     7441 ggtctgaagt atctccaaaa taaacttata taatataaaa atatacagag tattataata
     7501 taaaactttt aaatataaaa cttgaaagga aggaaaacct tatgaagatt agttctatgt
     7561 ttacacctat aagaattggt tcaatgacag ttccaaatcg ttttgtagtt ccccctatgg
     7621 gaaacaattt tgcaaacaca gacggtactt taagtgaaac ttctaaggct tattatcttg
     7681 agcgtgcttt aggtggtttt ggattaataa ctatagaatc tagtgttgta gacaaaaaag
     7741 caaaaggagg tcctcgtaag ccttgtttat atgatgatag cacaatagat agttttaaaa
     7801 atgtaataga tgcttgtcat gatgcaggtt cgaaggtatc tatacaatta caacatgctg
     7861 gttctgaagg aaatgaaaaa gtagcaggac atccattaaa agctgcttct gcaatacctg
     7921 catcaaatgg tcgtaataca ccactagcaa taacaacaga agaaatatat gaacttatag
     7981 aatcttatgg agatgctgca ttacgtgcac aaaaagctgg tgcagatgct gttgaagttc
     8041 actgcgctca tggatatctg gttagtagtt ttatatcaca aagaacaaat aagcgtgtag
     8101 atgagtttgg tggatgtttt gaaaatagaa tgagattacc tcgtcttatc attgaaaata
     8161 tacgtaaaaa agtaggaaat tctctagcaa tattatgtcg tataaacagc actgatgatg
     8221 ttccaggagg aattgatata catgatagtt cagtaatagc agcatatcta gaaggttgtg
     8281 gaatagatgg acttcatgtt tcacgtagta tacatataca tgatgaatat atgtgggctc
     8341 ctacgacttt acatgctgga ttcagtgcag aattagttac tgaaataaaa aaagctgtta
     8401 gtataccagt aataacagtt ggacgttata cagagccaca gtttgctgag ttaatggtca
     8461 gacaaggaag atgtgattta gtagcattcg gtagacaaag cttggctgac ccagaaatgc
     8521 ctaataaagc taaaaatgga aaactagacg aaatgatacc atgtattgca tgcttacaag
     8581 gatgtgtacc aaatatgttc caaggaaaac ctatagcctg tcttgcaaat cctatattag
     8641 gacatgaagc agagcttaag ccagctgaaa ttagcaaaga agttcttgtt gttggtggag
     8701 gtgttggagg tatgcttgca gcatgggtat gtgctaaaag aggacataat gtaactttag
     8761 ttgaaaaatc tgaagttctt ggtgggcaaa tgcgtttagc tgcatatcct ccagggaaag
     8821 gagatattac taacttagtt agaagttata tttcaaaatg taatcaatat ggtgtaaaaa
     8881 tatgtactaa tacagaagct acagtagaac ttataaagga gaaatcacct gatgtagtta
     8941 ttatagctac tggagcaact ccacttgtac ttcctatacc aggaatcaat gattcaggat
     9001 taattcatgc tgtagacctt cttgatggta aaaaatcttg tgggaaaaaa gtacttgttg
     9061 ttggaggtgg aatggttgga tgtgaagtgg cagccttcct tggagaacaa gaacatgagg
     9121 ttactgttat tgagcttaga gaagaagttg gtgcagatgt aatttcagaa caccgtaaat
     9181 tccttatgaa tgattttgaa aattataata tacataccat tactggtgca aaagtttcta
     9241 aattttatga tgatggtgtc gactatttac ttacagatgg cacagagcat gatttaaaag
     9301 gttttgattc agtagtactt gctatgggtt ctcgtaatta cgacccatta agtgaagtta
     9361 taaaagaagt tgtaaaagaa acttatattg ttggtgatgc agtaaaagct cgtagggcat
     9421 tagatgcaac aaaagaagct tttgaagtag cattaaatat ataaataata aataaatttt
     9481 tctttgtaaa taactgaata ggttatttgc aaagaaaatg ataatattgg gaggaaatta
     9541 tagttatgga aatttcaggt agaacaggat tatttgcatt aataggtaca ccagttggtc
     9601 actctaaatc accagtgatg tataattata gttttaagaa attagattta gattatagat
     9661 atttagcctt tgatatcaca gtagataagg taaaagaggc acttttagca ataaaaactt
     9721 ttaatataaa aggagccaat gttactatgc catgtaaatc tgctgttaca gaatatatgg
     9781 atgaactttc tccagcagct cgtataattg gagcatgtaa tacaattgta aatgacaatg
     9841 gaaagttagt tggtcatata acagatggag taggctatgt tcgtaattta aaagaaaatg
     9901 ggatagaagt taaaggtaaa aagattacaa ttatgggtgc tggtggtgca gcaactgcta
     9961 tacaagtaca atgtgcttta gatggagcaa gagaaatatc tatctttaat gctaaggatg
    10021 atttttataa aagagcagag cagacagttg agaatataaa aaaagatgta ccagaatgtg
    10081 ttgttaatct atatgattta gaagatacaa acaaacttta tgaggagata gaatcaagtg
    10141 atatattgac aaatgcaact cttattggta tgaagcctta tgacaatgaa acaaatataa
    10201 aagatactag tgttttaaga aaagacttag tagtaacaga tgtcgtttat aatcctaaga
    10261 aaacaaaaat gattgaagat gctgaagcta atggatgtaa ggctattggt ggattaggaa
    10321 tgttattata ccaaggagca gaagctttta atctatatac tggattagaa atgcctgtag
    10381 aagaagttaa tgaattatgc tttaaataga taagagtgat atcttaaaca atgaaaaaac
    10441 atacgtattt caatattttt aataataaat tacaaattgt catagtgggt tttgcatcat
    10501 agtccagtta aatttgaagc tttaactgac tatactttaa atccattatg aggatgagtt
    10561 tattgtacac aaaaatgtta ataaattaac aagaatggga gaaaaattat gaacaataaa
    10621 aaatattatc ctactgccat agcgttgtat ttttcatatt ttttacttgg tattggaatt
    10681 tcaattttag gacaatacaa accagaattt tcatcaatgt ggggggctaa aacactatca
    10741 gatggaacat tggatgtaag tattgtttta gctgttattg ctgcactagg attagggaga
    10801 cttatatctt atccatttgc aggacctatt tcagataagt atggaagaaa agtaagtggt
    10861 cttataggaa actttctaca tgcaattttc tttgttggaa ttgtattttc accaaatttt
    10921 tatatagcat atgtatttgc aataattgga ggggcagcaa attcattctt agatacctgt
    10981 gtaactcctt cttgtatgga gatatttgct agtttaggaa caatagcaaa tatgtttact
    11041 aaatttacaa tagctttagc acaatttttg cttcctttta taattggatt tgttgcagca
    11101 aattcaatat catttaaagt tatttttata gtaacagcga ttcttattgt agtagatgcc
    11161 attttgatag ctgtgttacc atttccacca gctaataatg ttattgataa caaaggcaaa
    11221 acagttaaat cagagaagat gaaatttaca ccaacaagta tagctcttgt ttgtattgga
    11281 tttacatgta catctacatt tgtattatgg ttaaattgta atcaggaatt gggtaaattg
    11341 tatgggatgg ctgaccctac aaaaattcaa tccttttact caatgggagt aatatgtgca
    11401 gtattaataa cttctttact tattaaaaaa tatattaaac caataagaat attagttata
    11461 tatcctataa tagctttact tatgctatta attgtatatt ttgttcaaac tccaactatt
    11521 tgtatgttag ggggatttgt aataggttat tcagcagcag gaggtgttct tcagttaaca
    11581 acgtcaactg caaatgaaat gtttccaact aataaaggaa aaataacatc tatagttatg
    11641 attgcatcta gtatagcaaa ctatgttatt ttaaatatag caggtattat tacaaaatca
    11701 gggggagtta atggaccaaa atatgttgta ttgtttaatg tggcaataac atttgttgga
    11761 attttacttg cattgtttgt aaacatgaga tatgaaaaag aaaaagtgta tgattatgat
    11821 gtctaatatt agtataatat atgcatagag aagtctaaat ctaggataga tttttattaa
    11881 aagtaattaa aattaataag gatattattt acagttataa gatagggtat ttacataatt
    11941 aataataagg atattactag aatttcaatt tctagtaata tttttttgcg tataaatgta
    12001 aacttgacga aataagtagt ataatcaata taaattacat catgtaaagg aggataattt
    12061 ggtggagaat gtttttaagc gattgcagga atttaatggt tatgatggat acaaagaatc
    12121 atttgaaatg aattacctat gtatatatga aagtataccg ttaagagaac aagtagaact
    12181 tgcaaataat ctagtagatg aaattttaaa tatgtataaa agtgaatcta atgaaattta
    12241 tttattggag gatagtaatt ctaagtcttt aatttgttat ttcgaaattt ttatgaaaaa
    12301 gataaatact ttggtaaaag aaatgattat tgatgagaaa tggttatata aattaactaa
    12361 agaattaata tataagagta aaaaagttga gtatgtaaaa ctagggttag ttttaagtga
    12421 gaaatatcta aatgtagaaa acttaagaga agttgtagat acattttcta aatctggaga
    12481 atacgtattt tatctgagta atactattaa aaaacttgaa ttttacaata catatttatt
    12541 taatttatct aaaaaggcta caggttcaat aaaagtattt gctattgtta atatggaaaa
    12601 tttagattct aaaataaatt cctatctaat tgaagatggt tataaggata caaagtacga
    12661 acgattattg atgaactata ttatttcaat tgtagatttg aatgaatatc ttgaaaaacg
    12721 agatttagat aaagaaaaga taaataattt agcacgtctt atatgcaatt acttgctaag
    12781 tgtagaattt aaatatatag gtaataaatt agaattagtg aatcgatttt tacctactgt
    12841 agtaaattat gggactaact ttgaaagttt atattcaatt ttcctaatag ctataaacgt
    12901 cttaaaagat gaaaatatag agtacaataa gattgaattt gaaaaagaaa taaatgatat
    12961 cttgctcagt gaaaaatgga aaaatatata ttttgaagca ttaagagatg ctagtggaaa
    13021 aacagaagat attataaaaa tgtcagaaat ttatgatgta aacttatctt ttgatgactt
    13081 gttgccatat ctgaatagag atattagaga ttttgaggta tattggcata tatctaaaaa
    13141 gggaacgaca agttctagat taaaattatt aaattttttt gaagagacgt ttaaaataga
    13201 tgatttaatt ggaaaaatga aagatataga aaaagataaa ttgactcaag aatattatga
    13261 tgatatgtta ttttttattg tgcttaaagg aagtaaatca ctatatcctg aaggaaagaa
    13321 tatatcttta aaaggaatat ttggaaatat aaatgaagta agaaaagaat ccataaatat
    13381 cttaaaaaga tacagagaga aactatctct tgaagaatta aaaattgtta aagaagcgta
    13441 tgaaaaagaa aaaaatgtta tattgaagga tgaattaaga agagtattat atgaaagtaa
    13501 taatttaaaa aaagaatttg taaatattga aaaaataaaa gtagatgaac atggtaagga
    13561 tatctatttg acttcaatag ctgttgctgg ttcaaggttt agaaatagag aatatcttga
    13621 aaaagaattg gagaagagta agatatacta tttaactaga gagaaagata atctatatga
    13681 tgaaaaagct attaaaatag ttggtgaaac tggatatgta ataggctatg tcccaagaaa
    13741 agaaaactat attttgagca atttattaga tggaggaaaa ctgctgtatt gtagagtaac
    13801 tgaatataat ttatatgaag attgtatcta cgcaaatgtg tacttgagtt ataaagatgt
    13861 aattgaaaca gttgaaaact ctttaaagat ggttttagat aagagtagaa taaaacttat
    13921 aaactaagac gtttatatat tgcttagatg atgtaatcct aactgattgg ttattttttc
    13981 tggaattatg gtgtatttta tgagcaatgt taatgaatat aaaataaaaa aacaattgtt
    14041 ttgtatatat gatgtgtata aatatattta tatattcata tttacaaaac aattgaaatt
    14101 tatgttattt tatgatttta aatttagatg gaattttaac ttctgcttta tcttttattt
    14161 tagaagcttg ttttttaact ttatttaata attcagattc atctacaact aatacttttc
    14221 tttccttcat caaccattta ccatcacaaa tagtagaatc tacattttca ctagtcatag
    14281 aataaactat atttccaatt ggattatgca ttggtaaaca atgaattgta tctggattta
    14341 gaataatcat atcagctttt tttcctactt caagactacc aatttctttt tctagtaaag
    14401 cacattttgc tccatttata gtagccattt ctaagacttc ttcagctggt acgacagttg
    14461 ggtttaaagt tctaccttta tgtattagtg atgtaagata catatccctc ataaggtcca
    14521 ttctattatt tgaaggagct ccatcagtac caattgaaac aggtattcct ttttctaaca
    14581 tttcaggaat tgaggcaaat cctagaacta ctttcatagc tgcacctgga ttatgagaaa
    14641 cttttacatc atgtaatcta aataaatcaa tttctttatg agtaagccaa actgtgtgag
    14701 cagctaaaag atttggtcct aatacaccta gtttatcaag atattcaact gttccaactc
    14761 cgttgttttg ctttacaaat gaaatttcat cagctatttc agcaatatgc atatgtatac
    14821 ctgtattgta tttatcagct aactctttag tcctaataag caattcttca gttgtattaa
    14881 aaatagttct taatgcaaac catattttaa tcctctcatt agatgtgtta ttaaacttat
    14941 ggaataagtc taattgagtg ttgatttcct catcagtagt tttttgccat gcttcaggaa
    15001 gaccatctcc tgtatccata actgatttag ctaaacaggc tctaagtcca gttttatcaa
    15061 cagcttcaac catagcatct acatattgac caccagattc taaaaatgta gttaccccag
    15121 attttatcaa ttcaacacaa catgcagtag atgaaatcaa tgaatcttca tagttgaagc
    15181 tactttcgta tggccagatt ctctctctaa gccaagttaa aagtacaaca tcatctgcaa
    15241 gaccacgtcc taattgttgg gataagtgaa cgtgtgtatt gactaaccct ggcattagta
    15301 tttttccttt agcatcatat atctcagcat ctttatctac aatattaata tcaaacttac
    15361 caacattttt tataatatta ttttcaataa gtatacttcc agtttcatat acctctctat
    15421 ctgcattcat agtaattaga tatgcatttt ttataagtaa ttgtgacata agtacaacct
    15481 cttttctata tat
//
LOCUS       DAEPLF010000071        15331 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEPLF010000071 DAEPLF010000000
VERSION     DAEPLF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.071"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            133..705
                     /locus_tag="KR314_001931"
     CDS             133..705
                     /locus_tag="KR314_001931"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBH0328314.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            769..1419
                     /gene="fsa"
                     /locus_tag="KR314_001932"
     CDS             769..1419
                     /gene="fsa"
                     /locus_tag="KR314_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBH0328315.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGAFLKL"
     gene            1571..4495
                     /locus_tag="KR314_001933"
     CDS             1571..4495
                     /locus_tag="KR314_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0328316.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYGTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
     gene            5084..5542
                     /locus_tag="KR314_001934"
     CDS             5084..5542
                     /locus_tag="KR314_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0328317.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFILGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLRTLKSL"
     gene            5603..5878
                     /locus_tag="KR314_001935"
     CDS             5603..5878
                     /locus_tag="KR314_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0328318.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            5937..7211
                     /locus_tag="KR314_001936"
     CDS             5937..7211
                     /locus_tag="KR314_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBH0328319.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            7341..8393
                     /locus_tag="KR314_001937"
     CDS             7341..8393
                     /locus_tag="KR314_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH0328320.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     AVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDIDPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            8393..9424
                     /locus_tag="KR314_001938"
     CDS             8393..9424
                     /locus_tag="KR314_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBH0328321.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKDIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            9516..10328
                     /locus_tag="KR314_001939"
     CDS             9516..10328
                     /locus_tag="KR314_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBH0328322.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            10331..11251
                     /locus_tag="KR314_001940"
     CDS             10331..11251
                     /locus_tag="KR314_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBH0328323.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            11426..11872
                     /gene="rpiB"
                     /locus_tag="KR314_001941"
     CDS             11426..11872
                     /gene="rpiB"
                     /locus_tag="KR314_001941"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBH0328324.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            11911..12576
                     /locus_tag="KR314_001942"
     CDS             11911..12576
                     /locus_tag="KR314_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBH0328325.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            12686..14188
                     /locus_tag="KR314_001943"
     CDS             12686..14188
                     /locus_tag="KR314_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBH0328326.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTNIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNNSNLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(14500..>15331)
                     /locus_tag="KR314_001944"
     CDS             complement(14500..>15331)
                     /locus_tag="KR314_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0328327.1"
                     /translation="INNISKNIKNIENGNLKYKLEVIGDDEISTLAKSINNITGGLET
                     AVDANIKNERMKAELITNVSHDLKTPLTSILSYVDMLKNNDFDRETINDYINILDKKS
                     QRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQSIVEFEDKIQNSSLDFIIETPE
                     YAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDMFIKEENIILTFKNISNDKLNL
                     KPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENAHMEIIIDADLFKVMLTFRRVN
                     "
BASE COUNT         6151 a         1637 c         2869 g         4674 t
ORIGIN      
        1 ctcctgtcta tgatatagac aggagctttt ttagtatttt aatataaaat acttacaaga
       61 atagatagca aaattagaac aaatataatt tttttattaa tataatttta gaaagggcta
      121 ggtggaagtt caatgaaggc tttacaagaa aagatattga gagaaggcag tgtttcagga
      181 aatgatatac taaaagttga tagtttttta aaccatcaaa ttgatgtagc ttttttaaat
      241 gaaataggaa gagaatttaa agagagattt aaaggagaaa aggtggataa gatatttaca
      301 atagaggctt caggaatagc tatagcatca attgtttcac agtattttga taatgcacct
      361 gtagtatttg caaaaaaatc agaatctaaa aatttggata aagatgtata tgaaactaat
      421 gtatattcat ttactaaggc aagagagtat tcagttaaag tttctaaaaa gtacataaac
      481 aaaggcgaaa atatattgat agtggatgat tttttggcaa atggaagagc tgctcttgga
      541 cttaaagatt taattgagca agcagaagct aacttagttg gagtaggaat tgtcattgaa
      601 aaaggatttc aagctggagg agcactttta aaggctaatg atgtaagatt agagtctttg
      661 gcagtagttg aatctataga taatggaact gtaaaattta ggtaatttat gatattatat
      721 aattgtgttt agcacatatt ttaactatgt aattatggag gattttttat gaggtttttt
      781 atagacacag ccaatattga agaaataaaa gaagcaaatg atttaggtgt aatatgtggt
      841 gtaactacta atccgtctct aatagctaaa gaaggtagag attttataga ggtagttaaa
      901 gaaataagtg aaatagtaga tgggccaata agcgcagaag taataagttt agaacataaa
      961 ggtatgatag aggaagcaga aaaattatct aagatacata aaaatatagt tataaaaata
     1021 cctatgacag cagaaggtct aaaagcggtg aaagtactat caagtaaagg tataaagact
     1081 aatgtaacgc ttatattttc tgctggacaa gctcttttag ctgctagagc tggagctaca
     1141 tatgtaagtc cttttgttgg tagattggat gatatatcac aaaatggtct tgatttaatt
     1201 gaagaaatcg ttgatatatt tagtgtaaat agtattgagg ctcaaataat agttgctagt
     1261 gttagaaatc caatacatgt tttacaagct gctagaatgg gagcagatat agcaacagtt
     1321 cctcttaaag ttataaacca aatgattaag catcctttga cagacaaagg aatagatagc
     1381 tttatgaagg attgggaagg tgctttttta aaattataat tataaactac gctttataag
     1441 tatattaaag aggttattat ggaaattcta agtaataagg ctaggctatt ttgcctagtc
     1501 ttattttggt gtataatata ttgaaacata ttattgaaat agattaattt tttaagtgag
     1561 gaggcgaaaa atggataaaa tattaagaga gatacaaaga gaagataaaa aaaatccata
     1621 tactgaccaa gaacttgctg aaatgttaaa tgtagctagg tctgaagtga tatcagttag
     1681 gaaaaaaaat aatattttag attcgagaga aagaagaaaa aaaatattaa taaaagatat
     1741 atcaaaaatt gtaagtgaaa atcctcaaat gtctgaaaga aaaataactg aaatattaat
     1801 agaaaatgga tataatatat cgagaagtgc agtttcaaaa ttattaaaag aggaaaattt
     1861 aaatcagtca aagattggga ttaaggataa agacccagca tcatcaggaa agtatgtgaa
     1921 ggtgagtaaa gttgtagaag aagatggatt tgaagaattg ataggtataa agggaagttt
     1981 gaaagaaaag gtaaatctag ctaagtcagc aataatgtat cctcctaatg gccttcatac
     2041 tataatatat ggagaaacag gagtaggtaa aagtgaatta gctacatgta tgtataaata
     2101 tgctataaaa aacaacatta aagaagagaa cagcccattt atagtgttta actgtgcaga
     2161 ctatgcagaa aatcctaatc tattgatagc tcaactcttt ggggtagtaa aaggtgcata
     2221 tacaggagca gatgcaaata gagaaggtct tgtagaacaa gctaataatg gaatattgtt
     2281 tttagatgaa attcatagat tgcctgctgc tgggcaagaa atcttatttt cattaataga
     2341 tagaggagaa tttagaaggt taggagaaag tagttctaat agaaaggcaa atgttctcat
     2401 aataagtgct acaacagaaa atccagattc taatttactt caaacattta gaagacgtat
     2461 accaatggta ataaatttac ctaatatgag tgaaagacca aaatctgaaa gatatgaaat
     2521 aataataaag ttttttgaaa gagaagcaaa gcgagtaaac aagaatttta taataactaa
     2581 agaagttatg tgtgcactta tgaattataa gtgtactgga aatataggtc agttaaagag
     2641 tgatatacaa gtaacttgtg ctagagcttt tagtaaatcg atattttcta gcgataaggt
     2701 aattatagat ttagatagtt taagggatta tataaaaagt ggatattatg attgtaattg
     2761 taaaaatgaa aagtattctg agttttcagt tgaggatata tatatagata ttagtgaatt
     2821 ggtgagtgga aaaaatagaa ttttatctga taatgagata agcgagatat ataattatgc
     2881 agaaaaagag cttaaggttt tggaaagtaa gtgcttttca gaagaagagt taaaaaatgc
     2941 atttatagaa aagttagata agaagtttaa tgctattaaa aacaataaaa atttatctga
     3001 tagaagaagt cgaatagact ggggaggaca attaaaagag tctacactgg agataatgga
     3061 taaggtaatt gtatttatta aggagcagtt taagcatgta aatcaaggtc tgtacttggc
     3121 tctagcaatt catattgagc atgctatagg taggataaaa gatggaaaaa ctataataaa
     3181 tccgagttta gataaaataa aagcatcaat gccaatggaa tatgagttgt caagatatat
     3241 aatgggtata gtagaagatt tgacacaagt aacatttcca gaagatgagc ttggatattt
     3301 ggcttattat ataaataaat tttgttataa tgaggaaagt ataaaagata aagttaaagt
     3361 agtaatagtt acgcatggta aagttggaat tgaaatgtct aaagttgtaa atcatatttt
     3421 aggtatagaa tgtacactag gtatagaaat agcacttaca gattcaccat cagaggggat
     3481 agagcatgta ttagaagagc ttcaaaagat agaagctaga aaaggtattc tagttctaat
     3541 tgatatgggt tctttggtaa ttcttggtga tgaggttgaa aaaaggcttg gaattagatg
     3601 taaaactgta aatagagtag atacattatt ggcaatggaa gcaggaaaac ttgcaaccat
     3661 tgaaggaaaa agtttagatg gtataatagc tgatttaaag aaaaataaga attatgctat
     3721 gataaataca aataaattta gttacagaaa aaacgagtat ggaaagaaaa atgtaatcat
     3781 tactttatgt ttgagtggtg taggtacagc tttaaattta aaagagcata tagaaaaaca
     3841 aattaaggaa tatggcactt taattgaagt aaagccagtt gcttttttaa ataataattt
     3901 agaagaagag ttaaatgata ttgaagctga atataatatt attgctatct gtggaactat
     3961 tgatatagat tataaaaacg tgccttttat ttcttataat gaggtacttg gtaaaaatgg
     4021 tattaataaa gtattagagc atctaaatag taaacaacca gctttaaata ttgaaaacca
     4081 gttgaataat ttaatacatg aagatttgat tttatacaat tttgaaggaa tttcaaagga
     4141 gtatataatt gatactcttg tttcaaaact tgaagaaggt ggatatgttg attcaaagta
     4201 tatattaagt gtatataaaa gagaagcaat gggaagtgtt gtaatggcct ctaaggtagc
     4261 cgtgccacat ggacttccag aaaacgttat aaagcctgca atagcaatcg caaggttgaa
     4321 taaacctata gtatgggaca ataaatttat ggtggatttg gtggtactac tggcacttaa
     4381 agaaaataat aagaaagaaa tacgaagctt attttcaaag attaatgacc agcatacttt
     4441 agaagtttta ttaaatactg aagataaaaa tgaaataaaa gaacttttaa gctaattatt
     4501 agtaccgaga agaaagacaa aaaaatttgc tttagtataa aatttaaaat tactcaattg
     4561 ctaagtagtt aattactcta tataaggcta gttctatgaa atttatttat atgattttat
     4621 agaattagct tttttcttgg taaataaaat atatgaattt atttatgtaa aaagtaaatt
     4681 ttattggaca attctatgaa aatttttcct atatttctta taaaatatag aatatataaa
     4741 aaatatataa aaataaatta aattcataat aaataatttt ataaaatagt tgttaaacat
     4801 aaaaaaccat ttgtaaaaaa caaaaaaatc atttctctaa caaaaaaaac cacaaaaaat
     4861 ataaaaaagt atggctagtt aagtgattaa caagtaaaaa ttaaaataaa aatcaaaaaa
     4921 aatttatcaa tgaaagtcag ctaaaatcct ggaagagagc attattttcg ggaaaacatt
     4981 ttcttttgtt tcacaaaaaa agccactttt tggcacgcaa catgctttat ataaagtcaa
     5041 gaaaaactaa ggtataagac aaaggcaatt aggaggtgta aatatggagt ttataaattt
     5101 tgtaagtgat aaagttatag accttaaaat ggattgtaaa acgaaagaag atttctttaa
     5161 agaaatacat aataaagttt ttgatttagg ctttgtaaaa gaagaatttg gagaaaaaat
     5221 attagcaaga gaaaatgtat ttccaacagg tttaaattta ggtgattatg gagtagcaat
     5281 accacataca gatgcagaat atattaaaga gcaatttata tcagtctgta cttttaatga
     5341 accagtagta tttagttcta tggaagacca agatgaaaaa gtacctgtaa atttggcttt
     5401 catattagga cttaatcaac cacacagtca attatctgta ttaacagaat taatgggaat
     5461 tatgcaaaat aaagagttag ttgataagtt gatgagttct actgataaag aagaagtttt
     5521 aaggacacta aagtctttat aaaaataaaa aagtactaaa aataaaaaat ataaaaataa
     5581 aaattattag gaggaaataa aaatgaaaaa aatattagta gcatgtggag caggaatagc
     5641 aacatcaaca gtagtttgtg acaaggtaga gagattagtt aaggaaaata atatacaagc
     5701 agaagttata caatgcaaaa tagctgaatg tgcaacaaag caagaaggag cagatttaat
     5761 agtatcaaca actatactac caacaactta taatatacca gcaataaaag caacagcata
     5821 tataactgga gtaaatacag cagcattaga taaaaaaata ttagatgctt taaaataatc
     5881 ctatcataga tacattgaaa taatatttta taaaacagaa aaaggaggat ttataaatgg
     5941 ataaattatt agcagtagta caatacattt taaatatggg accgacagta atactaccaa
     6001 taacaattgt agtactagga atgatattta gaattaagtt tacaaaagct ttaaagtcag
     6061 gtcttacaat tggtatagga tttgtgggta taaacttagt tgtaaaccta ttaacatcaa
     6121 cactagggcc agcggctcaa ggaatggttg aaagattcgg acttaattta acagtaatag
     6181 atgttggttg gccaacaaca gcatcagcaa catgggcttc accagttgca ccaatattaa
     6241 taccagtttg tttattagta aacttactat tattatactt caataaaaca aagatattaa
     6301 acgttgatat atggaattat tggcacttta tagtagcagg tgcagttgga tatatagtaa
     6361 caggaagtta tgtttgggct atattctgtg caatactaat ggagttatta gtactgttta
     6421 ttgcagaaaa gacagcaaaa aatgttcagg agttttatga tttagaaggt atagcattac
     6481 caactggttc aacagcttca tttgctccac ttggatattt agtaggtaaa tttgttgaaa
     6541 aaataccagg aataaataaa atacaagcag acccagactc tatacaaaaa agatttggta
     6601 tatttggaga accaatgatg atggggatta tactaggtat aatacttgga gcacttgcgg
     6661 gatatgatgt agcaggaata ttcaatgtag gtatatcaat gggtgcagta atgttactta
     6721 tgcctagaat ggttagaata ttaatggaag gtttaattcc aatttcagaa tcaataagag
     6781 atttcttaca agcaaaatat ggagacagag aactttatat aggtctggat gcagcagtag
     6841 caacgggaca tccagcagtt atatcaacag ctcttatact tgttcctata acgttgttct
     6901 tagcagttat actaccagga aacaaagttt taccatttgg agacttagcg acaataccat
     6961 tttatatggc atttatagtg tgctttagaa aaggtaatat agttcaatca gttataacag
     7021 gaacaatagt aatagctata tcactttata tggctacaaa ctttgcagat gttcatacac
     7081 aattattaca acaagcaaat atgatgccag aaggaacaac aatggttaca agtatagata
     7141 caggtggaaa cttgctaaaa tggggaatat tgaaattatc tcaattagtt tcttcatttg
     7201 gaatattcta atatgtagtt aatagagatt actctttttt agtaaagtat gcgaaaattt
     7261 aacatttagc agctactaaa aaagagtaat attttttata aatttttaaa aatacaaaat
     7321 acaagaggag gagatttatc gtgaaagcag cagtattaca tggcacaaat gatatgagat
     7381 ttgaagatat agaaataaaa ccatgtgaaa gtgatgaagt aaaaataaaa gttatggcag
     7441 caggaatatg tgggtcagat cctccaagag tattaaaaca ttggaaatat ccagttccag
     7501 ctataccagg acatgagttt tctggagtaa ttgctgaagt tggtaaggat gtaaaaaatg
     7561 taaaagttgg agatagagta gttgcaattc cttttatacc ttgtaatgaa tgtgaatatt
     7621 gtaaaagagg attattttca ttgtgtgatg accatggaat gttaggagct aaaagttttg
     7681 gagcttttgc agagtatgta aatataaaag ctacaaatgt acttccaata ggagatatgg
     7741 actttgaaga tgctgcaatg atagagccac tagcagttgc tatgcatgga gtacttaata
     7801 taggtgttca agttggagac gctgtagccg taatgggtag tggaacaatg gggcagttgg
     7861 taatacaagg acttaaaata gcaggagcag gaacaattat agcagttgat atatctgata
     7921 ataaattaag agaatcaaag gaattagggg ctgatataat aatcaatgct aaagatattg
     7981 acccagtaga aaaaataaaa gaacttactg gtggaaaagg cgttgacata gctcttgaat
     8041 gtgcaggttc aaaaataact caagaacaat gtttacttat aactaaaaag aaaagtaaga
     8101 taggattttt agggatagca tattcagata taactttatc agaagaagca tttgaaaata
     8161 tatttagaaa agaactagaa ttaaaaggtt tttggaactc ttattctgca ccatttccag
     8221 gacaagaatg gactaaggga atcaatctag ttaatgaagg aaagataaaa ttaaaagaaa
     8281 tggtatctca tagattctca cttgaagata cttacaaagc atttgaaatg ataagagata
     8341 gaaaagaaga atttaacaaa atattaattt taccacaagg ggttgaaaaa taatgaaagc
     8401 agtagttaaa actaaaccag gctatgataa tgtagaagtt ttagaagtag aagaaccaaa
     8461 agctacagga gacaaggtta aaataaaagt agaatatagt ggtatatgtg gttcagatat
     8521 acattcattt aaaggtgaat atgcaaatat aaaagcccca gtaacattag gtcatgaatt
     8581 ttctggaatt gttgtagaag taggagaaga tgttaaagat ataaaagtag gagatagagt
     8641 tacatcagaa actacttttg caacttgtga gaaatgtata tattgtgaaa ctaaagatta
     8701 taacttatgc ccaactagaa aaggtattgg tactcaagta aatggaagtt ttgctgagta
     8761 tgtactatca agagaagaaa gcatacatgt acttcctgaa aatgtatctt tattatcagc
     8821 agcccttaca gagccactag cttgttgtgt tcatgcagct cttgaaaaga ctactataga
     8881 aagttctgat acagttttaa taataggacc agggccaatt ggtttattat tagctcaagt
     8941 tgttaaatct caaggtgcta cagttataat gtctggtgtt acaaaagata aggaaagatt
     9001 agaaatagct aaaaatcttg gagtagatag aactgtaaat gtaatggaag aaagtttggc
     9061 agatgtagtg aatgaaatga ctgatggaaa tggagtaaac aaaggatttg attgttctgg
     9121 gtttatgcca gcagttaacg aagccttaag actgcttaga aaaaaaggta ctttcgtaca
     9181 agttggaatc tttgctaaaa aattaaatga aatggaccaa gaagcaataa tccaaagaga
     9241 gttacaatat ataggaagta gatctcaaaa gccaagttct tggattacag cattagattt
     9301 aatggcaagt gggaaagtaa atacagaagc tttagttact agaactgttg gtttagacga
     9361 atttaaagaa ggtattacag ctttaatgaa cggggaagaa ataaaagtag cagtaaaatc
     9421 ttaaaaaaac atttgtatta ttttaaaaaa aattgtaaaa tctactgtag gataaaaaaa
     9481 tgtaaggtct taaaaaataa caggaggaag aaagtatgag cactcttaga gatcattcta
     9541 ataatattag aaaaaacata ataaaaatgg ttgcagaagc taaaagcgga catccaggtg
     9601 gttcattatc aatagctgat atgttgacag tattatattt tgataagatg aacgtatgtg
     9661 cagaaaatcc aaaaatggct gatagagata gatttgttct atcaaaaggt catgcagcac
     9721 cagctttata tgctacatta gcagaaaaag gatttttccc agaagaagaa ttaataacac
     9781 taagaaaatt tggttctaag ttacaaggtc acccagacat gaaaaaagta gcaggaatcg
     9841 atatgtctac tggttctctt ggtcaagggc tatctgcagc aaatggtatg gcacttgcag
     9901 gaaaattaga taacaaagac tatacagtat atactatatt aggtgatgga gaaatccaag
     9961 aaggtcaaat atgggaagct gcaatgacag catcacatta caaattagat aatttaattg
    10021 catttgttga cttaaatgga ttacaaatag atggttctaa tgaagaagta atgaatgtaa
    10081 atccagtaga tgacaaattc aaatcttttg gatggaatgt aatagtaata aatggacatt
    10141 cttttgaaga aataggaaat gcagttgatg aagctaaaaa agttacagga aaaccaactg
    10201 taataatagc taaaacagta aaaggtaaag gtgtttcatt tatggaaaat aatgctgcat
    10261 ggcatggaac agctccaaat gcagaacaag cagagcaagc attgaaagat ttagaaggag
    10321 gtctatagga atgggaatag caactagaga agcttacgga caagtgttaa aagaacttgc
    10381 agaaaataaa gatatagtag tattagatgc agatttagga aaagctacaa aaagtatatc
    10441 atttaaagaa gttgccccag atagattttt tgacatgggg atagcagaag gtgacatgat
    10501 tggtacagca gcaggtcttg ctacttgtgg aaaaatacct tttgcgagta cttttgcaat
    10561 atttgcagca ggaagaggct atgagcaaat aagaaactct gtagcatatc caaatttaaa
    10621 tgtaaaaata gcagctactc atgcaggtgt aacagtaggt gaagatggtg gtagccatca
    10681 agctatagag gatatatctc taatgagagg tataccaaat atggttgtat taaatccagc
    10741 agatgcttta gaagcaagac aagctattct tgcatctatt gattataatg gacctgttta
    10801 tataagatta ggtagagctg caactcctga tgtaaacagt gaaaattata aatttgaaat
    10861 aggaaaagga actgttttaa gagaaggttc tgacataaca gtaatagcta caggaataat
    10921 ggtagcaaaa gccttagaag cagcagaaga acttgctaaa gaaggtataa atgtagaagt
    10981 tgttaatata tctacaataa aaccattaga tgaagcatta ataaaagaaa ctgctaagaa
    11041 aactggaaaa gtagttacag cagaggagca cagtataata ggtggtttag gttcagctgt
    11101 atgtgaagct ttagcagaaa ctaaagatgt agtagttaga agaattggtg ttaaagatgt
    11161 atttggtcaa tcaggtacac cagctgattt attaaaacac tatggtttaa ctacagaaga
    11221 tatagtaaaa aatataaaag agttgttata gatataaata tatctaaata acaaattgat
    11281 ttgacataag tcaattaaag ctgtcttaaa aatagaaaga aatttctaca atgggacagc
    11341 tttattttta caatacttta tttttatgat aattataaaa tagtctgaaa gtaatcaaaa
    11401 aaattataaa aagaggtgct atttaatgaa aatatctatt ggatgtgacc atggtggtta
    11461 tgaattaaag gaatatataa aggaaaattt aataaataaa ggtatagaag ttgaagatgt
    11521 gggtacaaat tcaaaagaaa gtgtagattt tccaatatat gcaaaaaagg taagtcaaaa
    11581 tgttcaaagt aaaaaatctg atttaggcat actttgttgt ggaacaggaa taggcatgtc
    11641 tattgcagca aataagttta aaggaatcag agcagctgtt gtttctgact gtttttcagc
    11701 taaggcaact agagaacaca ataatacaaa tgtactttgt ttaggagaaa gagttattgg
    11761 gaaaggatta gcaatgagaa ttgttgaaga atggttaaat tcaagttata atggagaaag
    11821 acatgcaaga agattacaaa tgattgatga tatagaaaaa gaaaatttat aaatatatat
    11881 aatatataat ttataacaag ggggagcgtt atgtctatta tttgtgcttc aataatgtgt
    11941 gcagaccaaa tgaagttaaa agaggaatta gaagctcttg aagaagcagg tgtaaaatta
    12001 cttcattgtg atgttatgga tggaatattt gttaaaaact tggctatggg accagagtta
    12061 ttaaaatcaa taagtgaaaa tacaacaata cctttagaca tacacttagc cacagaaact
    12121 ccagataaat atattgatat gatgtcttat ataaagccaa aatatatttc ttttcatgta
    12181 gaatcaagta caaatgtaaa agctgatata caaaagttaa gaaattatgg tatagggcca
    12241 gtgttagcaa taagccctca aacaagtgtg gataaaatag aagaatatat aagtttagtt
    12301 gagggtatct tagtaatgac agtaaatcca gggtttgcag gtcaaaagtt taatctttct
    12361 gtattggata agttggataa acttacagag atactaaaag attatgataa tcctccattt
    12421 atagaggttg atggaaatat aaataaggat actattaaac ttatgaatgg taaaaaagta
    12481 gatatatatg ttgttggtac ttcagcttta tttaatgata agcctccaat ttcatataaa
    12541 gataaaatag aagaactaaa agaaagtata aagtaaccat gtaataaaat tataatatgt
    12601 ttctgtttaa tagtaaaaat ttaggtggaa acaagaaatt ttataatatt taataaaaat
    12661 tttggttgag taaggagaat tttttatggg agacctatat aaactacaaa atggtactga
    12721 tattagagga atagcgtatg aaaatgattc taaagaagtt aacctaactg tagaagaagt
    12781 aaaaaaaata gctaaagcgt ttcatatttg gctaaaagaa aagactaaga aagataaagt
    12841 aactgttgcc ataggaactg attctaggat aacaggaagc caatttagaa gtactgttat
    12901 tgaaacttta actaatgatg attgtaatgt aattgattgt gagattgcta caactccagc
    12961 tatgtttatg acaactatta tggatggata caattctgat ggggcgatta tgattacagc
    13021 aagtcactta ccttattact ataatggttt aaagtttttc acagaaagtg gaggtttaga
    13081 aaaaactaat ataaaagaga tgttggatat agctgttaaa aatgatagtg tagactatga
    13141 ggaagtaaat aaaaaaggtg aagttatagt aaaaaattta atagaagatt attctaattt
    13201 attgatagat aaaattagaa aaggtgtaaa ttcaagtaaa aattatgaaa agcctttttc
    13261 tgggcttaag atattggttg atgcaggaaa tggagctggt ggattctttg ctgaaaaagt
    13321 tttacacata ctgggagcag atacgacagg cagtcagttt ttgaacccag atggtatgtt
    13381 cccaaatcat atacctaatc ccgaaaacaa agaagcaatg gaaagcatat gtaaagctgt
    13441 gttggataat aatagcaacc taggaataat ctttgataca gatgtagaca gagctgctat
    13501 tgtaggtaaa aatggcaaac caataaataa aaatgctctt atagctgtta tatcatcaat
    13561 agtattagaa gaacatccaa aaacagctat agtaacagat tcgattactt ctgaaggact
    13621 tgctaaattt ataaatgaat tacaaggaag acatcataga tttaaaagag gatataaaaa
    13681 tgtaataaat gaagctataa gattgaatag tgaaggtgaa gagtgccatt tagctataga
    13741 aacgtctgga catgctgctt taaaagaaaa ttatttctta gatgatgggg catatttaat
    13801 agctaaaatt ttaataaaag tagcaaagct atccttggaa ggaaagacta tagaagaatt
    13861 gatagaaaac cttgaagaag ctcaagaaga gaaagaagtt agaataggca ttaacaaaga
    13921 agattttaga ccatatgcag aggatatatt aaaagattta gattctcatg ttaagagtat
    13981 tgatggatgg tctgtagcac caaataattt tgaaggaata agagttaatt gtgatgaatc
    14041 aaatggagat ggatggtttt tacttagaat atcactacat gaaccattac tagctttaaa
    14101 tattgaatca aataaaattg gtggagcaga acaaatttat tctaagttaa aacttttctt
    14161 agaaaaatat gatttaaaag gtctttaggg taaatagaaa gaatatattt atattaaaga
    14221 acaaatgtaa atttgtacct aagattacaa atattacaaa attgtaatct taggtaacag
    14281 agatgtaact gattatggaa acactatgtg atattataga tacagagaga tgaaatatgt
    14341 gatgaattat agagaaatat aatttaaaat gtattaatat cacaccaaaa aggtataatc
    14401 aaaaaatatg taaatataaa ccccaacatt tagaaaaaga cctctaagga ggtttttttc
    14461 ttgtttaaat aacttaaaat atatttttat attaatatat caattaactc ttctaaaagt
    14521 aagcattacc ttaaataaat cagcatctat aattatttcc atgtgtgcat tttctaattc
    14581 aattatattt tgagcaattg ataaaccaag accagagcca tctgtttttc tagatatatc
    14641 accccttcta aatctttcta ttaactcttc tggctttaga tttagtttat catttgaaat
    14701 atttttaaac gttaatatta tattttcttc ctttataaac atatctatat agaccctagt
    14761 gttaggcata gagtatttta gtatattgca aattagattc tcaaaaactc tccaagtttt
    14821 tttaccatct atcatggtaa aaacagcata ttcaggtgtt tctattataa aatctaaact
    14881 gctattttga attttgtctt caaactctac aatactttga attaaaagtt ctcttatatc
    14941 ggtttttgtt atattaaatt ctacatctcc actacttagc ttagatgctt caaatatgtc
    15001 atctattaat aattttaatc tttgtgattt tttatctaaa atatttatat agtcatttat
    15061 agtctctctg tcaaagtcat tattttttaa catatcaaca tagcttaata tagatgtaag
    15121 tggagttttt aagtcatgag atacgtttgt gataagctct gccttcatcc tttcattttt
    15181 tatattagca tctacagcag tttcaagacc acctgttata ttatttatac ttttggctaa
    15241 agtagatatt tcatcatctc ctataacttc taatttatac tttaaattgc cattttctat
    15301 attttttata ttttttgata tgttatttat g
//
LOCUS       DAEPLF010000072        15159 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEPLF010000072 DAEPLF010000000
VERSION     DAEPLF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.072"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            93..2996
                     /locus_tag="KR314_001945"
     CDS             93..2996
                     /locus_tag="KR314_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0328328.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILVL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     ISIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3029..3475
                     /locus_tag="KR314_001946"
     CDS             3029..3475
                     /locus_tag="KR314_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0328329.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            3598..3900
                     /locus_tag="KR314_001947"
     CDS             3598..3900
                     /locus_tag="KR314_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0328330.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4081..5487
                     /locus_tag="KR314_001948"
     CDS             4081..5487
                     /locus_tag="KR314_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBH0328331.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFSDPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVISMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            5831..6889
                     /locus_tag="KR314_001949"
     CDS             5831..6889
                     /locus_tag="KR314_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH0328332.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYASPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            6964..7650
                     /gene="araD"
                     /locus_tag="KR314_001950"
     CDS             6964..7650
                     /gene="araD"
                     /locus_tag="KR314_001950"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBH0328333.1"
                     /translation="MLEILKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNVVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            7668..8315
                     /locus_tag="KR314_001951"
     CDS             7668..8315
                     /locus_tag="KR314_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBH0328334.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GIGREELNVEHICVLDLNGNVIEVEKGIKPSSEVLMHIEIYKIRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFESWKYPEEIKF"
     gene            complement(8488..9855)
                     /locus_tag="KR314_001952"
     CDS             complement(8488..9855)
                     /locus_tag="KR314_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBH0328335.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLRSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10045..10133)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10561..12681
                     /locus_tag="KR314_001953"
     CDS             10561..12681
                     /locus_tag="KR314_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBH0328336.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESILKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKEIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            12785..14038
                     /locus_tag="KR314_001954"
     CDS             12785..14038
                     /locus_tag="KR314_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBH0328337.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFEDIETYSKDSQKKREKEL
                     DKFINTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEIYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTQV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGFMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(14192..>15159)
                     /gene="ytvI"
                     /locus_tag="KR314_001955"
     CDS             complement(14192..>15159)
                     /gene="ytvI"
                     /locus_tag="KR314_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBH0328338.1"
                     /translation="LLMPFVISFIIASILRPIIRLITNNTNLNRTLISIVILLIFYGL
                     CIFLLVSFGVKIFASVSDVFFRLPEIYKSSIQPTLNTLFSKIDASTPNVNLSLILGWD
                     NISQSMMSLVASISTNALNAIASIASKTPAFMLKLIITLIASFFFTFDYQKIVNFILK
                     QFPEKSQFMIINIKNSSINALLKLLKAYAILLSVTFLELLIGLTILKVENAFTISVII
                     ALVDILPVLGTGSILTPWMIISLINGNINLAIGLLILYIIITVVRQILEPKVVGHQIG
                     LYPLITLMCMFIGAQLFGIAGLFGFPIAATIIKNLHDNGIITAFK"
BASE COUNT         6006 a         1730 c         2640 g         4783 t
ORIGIN      
        1 tagttactta cagggtttaa gtagaaaaaa atatcaaagt aagtatggca tgatagttgc
       61 ttatatattt atcaagaaag gagttattaa gcatggagtt gagagataaa cttgtaaaca
      121 tcataaatac agaaaataaa aaagatccat taacagatgt tcaaatagca aagtttttaa
      181 gtacaactag agagaacata actaatttga gaaaagaact taatataagt aattcaaggc
      241 aaagaagata tccttattta aaatcagcaa tttcggcaat attacaaaag aataagaata
      301 tttctatatc agaaattaca agggaattga tgacagaagg ctttaatata tcgagaagag
      361 ttgtggagga acttttacct aaagaaagtt tagaaatgga tgtagtagag gaatctgaag
      421 aagaatcgaa tgacccattt gaaactctga taggtaataa agggagtctt agaaatgctg
      481 tggagcaggc taaatctgct atattgtatc caccaaaagg attaccaacg ttgattattg
      541 gggaaagtgg agttggtaag agtttatttt ctagacatat gtatgagttt gcaaagcaaa
      601 aaaagatagt aaaagaaagc tccaattttg ttgtatttaa ctgtgctgat tatagtgaca
      661 atccacaatt attattatct ttgttatttg gatataaaaa aggtgcattt acaggtgctg
      721 aatatgatac accaggtctt gtagaagaag ctgatgaagg agttttattt ttggatgaaa
      781 tacatagatt gcctccaaaa ggtcaagaaa tactattttc tatcttagat aggggaaagt
      841 ttaggagatt aggtgaaact aatgctgaaa gaaaagtaag tattatgttg attggggcaa
      901 ctactgaaaa tgtagagact aatttattat taacattcag aagaaggata ccaatgctaa
      961 taactttgcc accattacat gatagattac taaaagagaa aatagacctt atatacaata
     1021 tctttcaaca agagtgtaat agaataaatg caaaaatatt tgtagataaa aatgtgattg
     1081 aaatacttgt actaaaaaag tttagtggaa atataggaca attacaaaat atgattcagg
     1141 ttctttgtgc aagagctttt atgaagttta taaattccaa agatgaagat agtgaatcga
     1201 tagtagtagt agatataaat gaagttttaa agcttaaaga ttcttttaaa gatgctgcct
     1261 ttcaagagat tgaatataca gagataagaa aatatttgaa aaatgctata tttataccat
     1321 ttaatttaga agaatcatca gtaaaaggaa gtcttttaag tgatgaatat atattaccag
     1381 aggacatata caaaacaatt gaaaagaaat attatgacct aaaatcccta gacattgaag
     1441 atatagatat agagaatatt ttatggactt ttattttaaa tagatttgct gacttaaagt
     1501 ttaatctcga tagtaatgaa gaaatttttt caatgagtga tttaaatagt tttgtaagtg
     1561 atagtttagt taaactggta aaagaactta tgaatgatat agttgaaaaa aatatgaatt
     1621 tagaagtaaa taaaaatatg ttcaaatatc tagcaataca cttagaagaa gctataaaaa
     1681 gaattaagct aaatcaaaag ataataaatg taaatttaga aaaaataaag attgactttt
     1741 caaaagaata tgaaattagt aaaagttttg caataaaaat tgaggaatca attagtatta
     1801 agattccaga tgatgaaatt ggatttataa cactctacat aaagtctgct ctaaaaaatg
     1861 aagtgaaaaa agataaagta gggttaataa taatttctca tggacgtata gctacagaga
     1921 ctgtaaatgt agttaaagaa cttttaggtg taaagtttcc tgtggctata gatatgcctt
     1981 tagatgaaaa accaataaat atatacaata aggcagtaga actgtcaaaa ataatagacc
     2041 aagggaaagg tatattgttt ttggtggata ttggttcact tacaaacata ggtcaaatag
     2101 taaataagag aaccaatata agcactaaaa ctattgatag agtagacctc ctaatggcac
     2161 tagaagcaac tagaaaagtt tctatgggag aggaagaact tgatgaaata tttttttctt
     2221 tgctaaaaga taggatggga tataactatg aattagaaaa acacatagat aaatcaaatg
     2281 caattgtaac attatgtctt actggagaag gaactgctaa gtatataagt aaaacgttgg
     2341 aagaaaagta tgataacact aaatgttatc aaatgagtgc attggatgaa aatttatttt
     2401 gtaaaattga aaatctaaaa gagacaaaca atatattagc tattgttggg acaataaacc
     2461 ctaagatacc aggtataaat tttataccct ataataaaga acttttcaaa aatttggata
     2521 tctatctatc aaaatcaaaa caacaagatg acagaccagt taaatcgtat gaaagaatgc
     2581 ttgatgagga tttagtgatt tttgaaccag atatatattt taaaaaggat ttattagaat
     2641 atgtatgttc aattcttata aataaagaat atgtagaaaa agattactta gattctgttt
     2701 tacatagaga agaaatgctt ccaacatatt ctaaaggagc tataggtgtg cctcatggag
     2761 attcttctac tgtaaatagt acaaggtttg tttttgttaa actaaagaat cctattgatt
     2821 ggggtgtagg taatgtaaat ttcatattta tgccagtttt tcaggctaat gataaagaga
     2881 ttgttaaaaa tgttcttgag atactaaaag atgcagaatt tatgaacaat gccaataagt
     2941 gttttgataa agatgctttt agaaggatta tatttgataa atttaaacat ctgtagccat
     3001 agcaaaaaaa ttgagaggag ttagagtaat gaatgtagat ttaatatcat tgggtttaga
     3061 atctaatgat tatgaaagtg ttattgaaga attaggaagt ataatgtgta aaaaagaata
     3121 tgtaaaagaa acatacataa atgctgtctt ggaaagagaa aggacattac caacaggtct
     3181 agatattgga gaaatgtgtg ttgctattcc acatacagat tcaaagcatg taaatgagtc
     3241 taatgtagct gtagggatat tgaaaaatcc tgtaaagttt aattcaatga tagacccaaa
     3301 agatagactt gatgtagaac ttgtattttt attagcagta aagaacccag attcacaagt
     3361 aaaactgtta aaagatttaa tgtcagtttt tcaaaatata aagttattaa aaaatataaa
     3421 aaacgcatca acaaaagaag aagtagctaa attattggat tttatagaaa tttaatgtag
     3481 gagaatttta taaataaaaa attaaaattt caacaatcaa agtaagtatt atttttttaa
     3541 taggtttaat cttatataaa ataaaaattt agagtgaaaa attaggagga aattattatg
     3601 aaacaattta atgtattatc agtttgtggt tcagggacag ttacatcatc tatggtagca
     3661 ggtaaattaa aagaagtact aggtaataag gggatttcta ttactacaac agaagctaga
     3721 ccaacagaag cactaaattt agcacaatca ggaagatttg actttataac atttacgagt
     3781 ccgcttcaaa caggcgatta tggaatacca actataaatg catttgcttg cctaactggt
     3841 ataggggaag aggcattctt tgaagaagtt ttgactgtta ttgaaggcat agataagtaa
     3901 gttttggaga cgtgaattta tagtatttaa atcaatgatt tagatactat aataagctaa
     3961 tattaactaa atatttgtgg aaaaaacatt ttatatgttg tcataaatta aaaaattata
     4021 aaaatagtca cttaagtatt aaattaagct tataaatatt tataagtagg agggaaaaag
     4081 atgttagaat ttatgaaatc tataatcgat acatttggtt ctgcaattat agttccaatt
     4141 ataatattca taatagcaaa gatatttaaa gtaactacta agaaatcgtt tctttctgct
     4201 gtgtatgcag gagttgcttt acaaggtttt acactgattt taaattcatt tacaccaata
     4261 ataacaccag ttatcaatag gatggttgag agtacaggag taaacctacc agtttttgat
     4321 gttggttggc aagcaacatc attggttgca ttttcaacaa gtgcaggtat gatatattta
     4381 ggtcttggta tattattaca aacaatttta tttttgataa aatggacaga cgtgtttcaa
     4441 ccatcagatt tatggaataa ctattcttat atggtatggg gagcaatggt tataggagta
     4501 acaggaaatt ttcctttagg tatagcatgt atggttttat taaacttata tagtttatta
     4561 atatcagagt tagttgcaaa gagatggtct agttactata gatatcctaa ttgtacaata
     4621 atagctatgc ataatgttga ggcttctgtg tttgcagtat tttcagaccc tatatacaat
     4681 aaattgggat taaataaaat aaaattaaat ccaaaagaat tagagaagaa attagggttt
     4741 ttaggtgaac caattacttt aggtcttttt ttaggtatgt ttataggtat attaggtaat
     4801 atgacgagaa taaatactat ggaagcttgg ggagaaataa tgaaagtagg tatatctaca
     4861 agtgcagtga tggcgatttt ccctaaagta gcaagtatgt ttgctcaagc atttgcacct
     4921 ataacagaag cagctagaaa gattatgcaa aaagctggaa accgtgagtg gtacatagca
     4981 gtaaatgatg ctgttggata tggtgaacct gcaactttaa tatcaggtct tatattaatt
     5041 ccaataatgc ttgtaatatc tatggtactt cctggtaata aggtattacc agttgtggat
     5101 ttgttggcaa ttccatatat ggttcaaggt ttagttgcca tacataatgg taatatacca
     5161 aaagtattag tttcaggtat aatttggttt ggattaggat tatatgtttg tacatctaca
     5221 gcaccattat ttacagatat ggctacaaat ataggtgtag ctatacctgc aggagcaatg
     5281 ttaataacaa gctttaatat actaggaaaa cctttgatgg gattagtttt ctttgcattt
     5341 ctaagtgcaa atccaattta tataggtctt tcagttgtaa tttactttgt tctatgggca
     5401 ttgtttagaa agaacaaaac aagtattctt gattatttag aaaaacaagc acttaaaaat
     5461 gtagaagaag agcctgtagc agtttaagtt tataaaaatg aaataagaat aattaaagga
     5521 gtttataatt gattttatat aagtatcaga taatacctga gaaatattta aatgaaatat
     5581 agacttcttt ttatttgata atatttgtaa ttataatgca ttgtattttt atagttgagt
     5641 tttctgtata acctgctatt taaaacatac tcttttatat ttatacagta atgtggttta
     5701 attttataaa atgtaatctc tactggtaaa agactttgta tataaggcat ttgcctgaat
     5761 aaaccattta tcttttgtat ggcatagata ttgctatata gaagtatata gcatgaaagg
     5821 agatatttat atgggagata aaatgagagc atcagtattg tataatgtag gtgatgtcag
     5881 gtatgaaatg gtagatatac cagaaataac agatacgcaa gtgcttgtaa atgtaaaata
     5941 tgttgggata tgtggttcag atttgcctag gtctatggta agtggtttga gtggaaatac
     6001 aaaatatcca ttgattttag gacatgaatt ttctggtgaa gttgttaaaa ttggagaaaa
     6061 agtaaagcat attaatgtag gagatagagt agctgttgca ccattagtac cttgtggaaa
     6121 atgtgattat tgtaatgaag gtaattttgg cttgtgtgat gattacaata taattggaac
     6181 aagagtaaat ggagcgtttg cagaatatgt aagagttcca gaagaacata ttttaaaact
     6241 accagataca ttggattatg aaactgctgc cggaattgaa ccagcaacta tagcttatca
     6301 tgggatttca aagtctaata ttagagtagg agatagtgta gttgtgttag gatgtggtcc
     6361 aataggtcag tttgttatac aatgggcaaa ggtgtttggt gcatctaaga ttatagcagt
     6421 tgatattttt gatgaaaaat tagaactttc taaacttcta ggagcaaact atatacttaa
     6481 ctcaaaagaa gtaaatgtaa tcaaagaaat aaaaaaaata acaaatggtg gtgctgatgt
     6541 tgtaatagag actgctggta gccgatttac acaagaacaa tctctatttg tagcaaaaaa
     6601 gagaggaaat atagtgtttg taggtatatc acacacagaa ttaccactta gtgcagacgc
     6661 tactgagtgt atattacgtg gagaacttac cttgaaaggt tcttggaatt catatgcaag
     6721 cccttatcct ggaagagcat ggactgcaac attggatttt atgggaaaag gtgacattat
     6781 ttttaaaccc atgatttcac ataaaatagg tcttaatgaa gtgggtgact ttttaagtaa
     6841 aatgagcaag agagaaatta atttcaataa aatcctagta gaaatataaa aatttaataa
     6901 aaaattctaa aaacaattaa attttataac tatgacatta aataaaatat aggaggaaga
     6961 aaagtgctag aaattttaaa agaagaagta ttaaaagcaa acttagaatt acctaagaaa
     7021 aagctaatta cttatacttg gggaaatgta agtgggatag atagagaaaa aggattagtg
     7081 gtgataaaac caagtggtgt agagtatagt aatatgactg tagaagatat ggttgtagtt
     7141 gatttagagg gaaatgtagt tgatggcaaa ctaagaccct cctcagatac tccgactcat
     7201 ctagttttat ataaagagtt tgaagaattg gggggaatag tacatacaca ttcaagttgg
     7261 gcaacaattt gggctcaaat aggaaaatct ataccatctt gtggtactac tcatgcagat
     7321 tatttttatg gaagcatacc atgtaccaga aaaatgacta gagaagaaat ttgcaatgaa
     7381 tacgaaaagg aaactggaaa tgtaatcatt gaagaattta tgggaagaaa tccaattcat
     7441 tgtccaggaa taattgtaaa tgaccacgga ccttttacat ggggaaaaga tgcaaatgag
     7501 gctgtacaca atgcagtagt tttagaagaa gtcgcaaaga tggcttatta tacagagtta
     7561 atgagtcctg ataatattat ggataaggtt cttatgaata agcatttttc aagaaaacat
     7621 ggaaaaaatg catactatgg tcaaaaatga ttatctggag gtgaattatg ctagactctt
     7681 taaaaggtaa agttgtggaa atagcaatta aagcagagca ttatggttta tgtaaagaaa
     7741 agactggtaa ttttagtatt agagataagg atactggata tatacttata accccatcag
     7801 gtataggaag agaagaatta aacgttgaac atatttgtgt attggatttg aatggaaatg
     7861 taatagaagt tgagaaaggt attaagccaa gtagtgaggt attgatgcat attgaaatat
     7921 ataaaataag ggaaaatata aatgctatat cacatactca ttctttatat gcaacagcat
     7981 tttcagtagc aaagaaacca attataccaa ttgtatatga atcagttaat tatggcggtt
     8041 atgtagatat tgctccatac gaaagaccag gaactttaaa attagctaag agcgtttcaa
     8101 aattactgat agatacagat gcatgcttat tagaaagaca tgggttgaca actgtaggta
     8161 atgatttaga cgaagcatta ttaaaatcaa gatatgttga agaggtagca gaaatatact
     8221 ataggtcttt agtattaaat caatttaaag aacttgaagt agtagatgtt gatgagtttg
     8281 aatcttggaa atacccagaa gaaataaagt tttaatctaa ataattttca ttgaagtggt
     8341 taattaaaat atatgtatta tttatatcaa ttactatgtt ttattatatt aatcaccgta
     8401 ttttgtaaat atatttatta cattttttca aaaaaatata gaatccgaaa gccatataaa
     8461 attgacttgg gattctatac tttttattta tgcatttttc tttatctgat tttctggaga
     8521 atttatatgt tcaaaatatt ttttagtttc agatactatt ataggtgata atgcaagtaa
     8581 agctataagg ttaggtattg ccataagccc atttacaata tcagcaatta tccaaacaac
     8641 ttctaacttt aagaaagcac ctaataacac catagccaca aatacagttc tatacccatt
     8701 catgcctttt acaccaaaca gaaattcaaa acatctttct ccataataac tccatcccaa
     8761 aattgtagta aatgcaaata atgttaaact aactgtaagt aatattggtc caaatgtagg
     8821 aattgctccg ttaaaagctg cttgtgtcat aacagctcca tttaggtctg aacaccacac
     8881 tcctgaaata acaagagaga atccagtaag agtacagatt ataatagtat ctataaaagt
     8941 acctgtcata gatataagac cttgttccgc tggccattca gttttagcag cagctgcagc
     9001 tattggagca ctacctagac cagactcatt tgaaaataca ccacgagcaa taccatttct
     9061 aattgccagt gacatagttg ctcctaaaaa accaccagta gcagcagtag gtgtaaatgc
     9121 acttttaatt attaacataa aagtttcagg gattttattc caaaaagata ttaaaatgat
     9181 tccacagatt acaacatata taactgccat aaaaggtact attttagtag ctacattaga
     9241 tatacttcta agtcctccaa atacaaccaa agctacaaat actgctaata caacccctgt
     9301 aattctaggg tctatcccga aactattatt tattgcatct gttattgaat ttacttgagt
     9361 aaaagtaccg attcctaaaa gtgcaactag tataccactt atagcgaaaa atattgcaag
     9421 aggtctccat ttttctccca taccatttac tatgtaatac attggcccac cagacacttg
     9481 accattttta tctttagttc tgtattttat tgcaagaaca ccttctgaat acttagtagc
     9541 cattccaaag aatgctgcta tccacatcca aaaaagtgca ccaggtccac cagctttgat
     9601 agctgtagca acaccaacaa tattaccagt accaactgta gcagctagag cagtacataa
     9661 ggctgcaaaa cttgaaacat caccactacc tttgttttca gcagagaata ttaacttaag
     9721 agctcttggc agtttagtta tttgaacaac tccaagctta acagtaagta gtataccagt
     9781 accaactaat agtaccagta ggggtggtcc ccaaataaaa gcatcaactt tgtttaaaat
     9841 atcaattaga ttcataaaaa cactccctca ttttttttgc tttacataat caatagaatt
     9901 tttaatgaag taattgacag gaaataagta taatttatta ataatttcta gtgtaataaa
     9961 aaaagctaaa tctaaaaaag attcagctct ggatgtacac taattagcaa ccaaaataaa
    10021 agtattagta aatactgttt ttggtagata atccctgtcc ttttgcctga gagattgagc
    10081 ataaaagcct tgccccttcg gcgcccataa tgggtctctc cagagttccg taaattttac
    10141 agtcctactt ttaaaagtac ctgagagtgt tactccttcg gtgtgctata tagcaactct
    10201 tctataaaat cttaaccgga tattgatttg tcatagctta ttgtattaat aatattaata
    10261 taagttttta tgttttgtca actaaaaaat taaaaaacaa agaaaaaata taaaaatgta
    10321 agcctattta cgaattataa acttagaaag atgagtaata tacgtaaaaa tgaattttgt
    10381 aatacacata caaatgaagt gtaaaataag ttttaaataa aaaaataata aaatcttaaa
    10441 taattaatta gatatgatta aattatggta atattatagt tacgatatta agaaaagttc
    10501 tatttagtta gattttaagc gtaatatgag attatgtata gagaaaggat gtatttttag
    10561 atgggaaaaa tattagtgct agcagaaaaa ccttctgttg gaagagatat agcaaaagtt
    10621 ttagggtcaa aaaatgaaaa aaatggttat atagaggggc caaagtatgt gataacttgg
    10681 gcactaggtc atttggttac acttgctgac cccgaaagtt atggagaaag atataaatca
    10741 tggagcttag aagacctgcc aatacttccc aagcacctta agactgttgt aataaaaaag
    10801 agtggaaagc agtttaatac agttaaatcg caaatgaata gaaatgatat agatgaaata
    10861 gttatagcaa cagatgcagg tcgtgaagga gaacttgtag caaggtggat aatagaaaaa
    10921 tcacaggtaa aaaaacctat aaaacgtctt tggatttctt caagtacaga caaagcaata
    10981 aaagacggat ttgcaaaatt aagaagtgga aaagaatatg aaaatttgta ttactcagca
    11041 atagcacgag cagaagctga ttggattatt ggaataaatg caaccagagc tttgacaaca
    11101 aagtacaatg cacagctttc ttgtggaaga gtacaaactc ctactcttgc aatgttgctt
    11161 aaaagagaag aagaaataag aaatttcaaa cctaaagagt attatggatt agaattaatt
    11221 gctacaaagg gaaattcaga tataaaattt atttggaatg ataaaaacaa taattcttca
    11281 acttttagta aagaaaagat agaatccata ttaaaaaaag taaaaggtgt agacataaaa
    11341 atatctgaag taaacaagtc atataagaaa aaatattcac ctgcactcta tgatttaaca
    11401 gaattacaaa gagatgcaaa taaaatgttt ggattttcag caaaagaaac tttatctata
    11461 atgcaaaaac tgtatgaaca tcataaagta cttacttatc ctagaactga ttctagatat
    11521 ttaacatcag atattgttgg aacattaaaa gatagaataa aagctgtaaa tattagtgat
    11581 tattcaaaga tttgtaataa attattaaaa agcaatatta tagctaataa atcgtttgtt
    11641 gacaattcaa aggtaacaga ccaccatgca ataataccaa cagaacagaa ggtattcatg
    11701 agtgatttta gtgacaaaga gcgaaaaata tataatcttg tagtaaaaag atttttagca
    11761 gtactatctc ctccttttga atatgagcag acaactataa gtgcaactgt agaaggagaa
    11821 atatttaagg ctaagggaaa caaagtaata aatttaggtt ggaaagaaag ttataaagat
    11881 attgaagatg aagatgagta tgaaagtata ccaaatgttt tagaaaaaga tgtacttaag
    11941 gtcaagggat taaagataac ttcaggaaaa acaaatccac caccatactt aaatgaggga
    12001 acattattga ctgctatgga aaagaatggt ctagggacag ttgctacaag agcagatata
    12061 atagaaaaat tattcaattc atttttaatg gaaaagaaaa ataaagagat atatataaca
    12121 tcaaaaggaa gacaattatt agatttagtt ccagtagact taaagacacc agaacttaca
    12181 gcttcatggg aaaagaaatt attggatatt tcaaagggaa aacttagtaa aaatacattc
    12241 ataaatgaca tgaaagatta ttcaaaagat attgtactag aagtaaaaaa tagtgataat
    12301 aaatttaaac atgataactt aactaaaaat agatgtccag aatgtggtaa atttatgtta
    12361 gaggtaaatg gtaaaaaagg taagatgttg atttgtgaag atagagagtg taatactaga
    12421 aaaactatat cacaagtaac caattcaaga tgtcctaact gtcataaaaa attagaactt
    12481 cgtggagaag gtgaggggag aatcttcact tgtagttgcg gatacagaga aaagttgtct
    12541 tctttcaaca agagaaagca agaagaaaaa cagaaggcat caaaaaaaga tgttaatcaa
    12601 tatttaaaaa atcaaaataa aaatcaagag acatttaata atccatttgc agaagcatta
    12661 gctaagctaa aaaacaaata attttaaagt tattattata cacaattata actttaactg
    12721 tatattagtt atatgtactg atataatata gtgtatagtt taggcatgaa agtagggctg
    12781 ttaaatggat ttaagtggca tatttaaata ctattgtaaa gagtgtgaaa atacatggaa
    12841 taattcgagt gttgaattat ttgaggatat agaaacgtat agtaaagatt cacaaaaaaa
    12901 gagggaaaaa gaattagata aatttataaa tacaatatca gttcatttag agaggtatcc
    12961 aagtgatgct gtattgagaa aaatgtgggt aaaaaagggc gaggttttct tacaaaagac
    13021 attggaaaaa gaaaatattt ttaagttaga aaaaatggat gtagaggata gaaaaaaatt
    13081 tttagatata acaaaacagt ttattagaga tgctagaaaa tttgatgatg atttacctat
    13141 aggtgatatt atgcaagcta tgaggaatgt atggatttca aatgcattac aattattatt
    13201 tggtaaagaa atatattatt caaaagctaa ctttgcatat agtatgttat atccatatac
    13261 tgataattat ttagacaata caaatataga taaaaatgat aagattttat ttaataactg
    13321 gttagaaaaa aggctcctgg gagaacatat taaatctaag gattatcatg aaagtaaagt
    13381 atctcaaatg atagattata ttgaaagtgt ataccctaga gaaaagttta cacaagttta
    13441 tgaatcgtta ttattaatat ttaaaagtca agtaaatagt ttaaaacaac atggtaagga
    13501 aaatcatttg tgtaaagaag atttattatc catttctatt gaaaaaggag gttcatccgt
    13561 tttagtagat ggatatttaa taagtggatt tatgacaaag gaagaaatag agttttgtat
    13621 aggatatgga tttttattac aaatatctga tgatttacaa gatataaaag aagatttaaa
    13681 atacaaccat aaaactatta ttacagagat gtcaaaagag ggtactttag ataaagttgt
    13741 aaataaacta ataaatttta ctattgagtt aatagatagt tttaaaatta ataataaaaa
    13801 taaatctgta ataactatga taaagaatga ttgcttaatg ttaattttat tttctgtagt
    13861 ttataatgct gaattttttt ctgtaggata tataaaagaa gtagagaaat ttattccata
    13921 tacaatagat tattcattag agattgaaga aaaaataaaa gaaaaattta aaaatataga
    13981 tgttttaaat aatgaaaatg aatataaaga aatgattgat attatttgtg cagagtagaa
    14041 aaaaagggag tgtcttagaa tagacttttt agtttatgag gcactctttt tgtataaaat
    14101 caaatgtatt ttcatcattt taacaattaa aaagagctta tccctgtttg agaaagccct
    14161 ttttaattta aataaaaatg tcaaggttaa actatttaaa tgcagtaata atcccattat
    14221 catgtaagtt ttttatgatt gtagctgcga tagggaatcc aaataatcca gctattccaa
    14281 atagttgagc tccaataaac atacacatta atgttattaa aggatatagg ccaatttggt
    14341 gtcctactac ctttggttct aatatttgtc taacaacggt tataattatg tatagtatta
    14401 aaagtcctat tgctaaatta atatttccat taatgagtga gataatcatc caaggtgtca
    14461 aaatacttcc tgtacccaag actggcaaaa tatcaacaag tgctatgatg acagatatag
    14521 taaatgcatt ttcaaccttt aggatagtaa gtccaataag tagttctaag aatgtcactg
    14581 atagtagtat tgcataggct tttagtagtt ttaaaagagc attaatactg ctatttttta
    14641 tatttattat cataaattga cttttttctg gaaattgttt taagataaag tttactattt
    14701 tttgatagtc aaaagtaaag aaaaaagaag caattaaagt aataataagt tttaacataa
    14761 atgcaggtgt tttacttgca atactagcaa tagcattgag tgcatttgtt gaaatagaag
    14821 ctactaaaga catcatagat tgacttatat tatcccaacc aagtattaat gataggttaa
    14881 cattaggtgt actagcatct atctttgaaa ataaagtatt tagcgttggt tgtatacttg
    14941 atttatatat ttcaggaagt ctaaaaaata catcacttac acttgcaaat atcttcacac
    15001 caaagctaac taataaaaat atacacaacc cataaaaaat taaaagtatt actatagaaa
    15061 taagtgtacg atttaaattt gtattatttg taataagtct tataataggt cttaagatgg
    15121 atgcaatgat aaaactgatt acaaaaggca ttaataatg
//
LOCUS       DAEPLF010000073        14676 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEPLF010000073 DAEPLF010000000
VERSION     DAEPLF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14676
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.073"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..2666)
                     /locus_tag="KR314_001956"
     CDS             complement(<1..2666)
                     /locus_tag="KR314_001956"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBH0328339.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKAHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKVKKEKFEEMIKSVEERLTNLESKIK"
     misc_binding    complement(2742..3004)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3305..4132)
                     /locus_tag="KR314_001957"
     CDS             complement(3305..4132)
                     /locus_tag="KR314_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0328340.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(4180..5616)
                     /locus_tag="KR314_001958"
     CDS             complement(4180..5616)
                     /locus_tag="KR314_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBH0328341.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(5703..6572)
                     /locus_tag="KR314_001959"
     CDS             complement(5703..6572)
                     /locus_tag="KR314_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBH0328342.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGNYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(6730..7383)
                     /gene="phoU"
                     /locus_tag="KR314_001960"
     CDS             complement(6730..7383)
                     /gene="phoU"
                     /locus_tag="KR314_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBH0328343.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(7411..8175)
                     /gene="pstB"
                     /locus_tag="KR314_001961"
     CDS             complement(7411..8175)
                     /gene="pstB"
                     /locus_tag="KR314_001961"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBH0328344.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(8194..9018)
                     /gene="pstA"
                     /locus_tag="KR314_001962"
     CDS             complement(8194..9018)
                     /gene="pstA"
                     /locus_tag="KR314_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBH0328345.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVIIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(9020..9937)
                     /gene="pstC"
                     /locus_tag="KR314_001963"
     CDS             complement(9020..9937)
                     /gene="pstC"
                     /locus_tag="KR314_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBH0328346.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(10319..11554)
                     /locus_tag="KR314_001964"
     CDS             complement(10319..11554)
                     /locus_tag="KR314_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0328347.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DNNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDIYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAITAPTF
                     SGEYQTQSDSILRCTKHGHTRLAFTKILALFSIFIVMFVVCISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGLKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
     gene            complement(11555..12442)
                     /locus_tag="KR314_001965"
     CDS             complement(11555..12442)
                     /locus_tag="KR314_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328348.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYIMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            complement(12587..13702)
                     /locus_tag="KR314_001966"
     CDS             complement(12587..13702)
                     /locus_tag="KR314_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0328349.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPNMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            complement(13738..14436)
                     /gene="vanR"
                     /locus_tag="KR314_001967"
     CDS             complement(13738..14436)
                     /gene="vanR"
                     /locus_tag="KR314_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBH0328350.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
BASE COUNT         4608 a         2673 c         1627 g         5768 t
ORIGIN      
        1 tattttattt tagattctaa atttgttaat ctttcctcaa cagatttaat catctcttca
       61 aatttttctt tttttacttt ttcttcttca attaaacttt caggagcttt tgctaagaaa
      121 ccttgatttg caagctttcc attaactctc tttatttcgc cttctaactt tttcttttct
      181 ttagacaatc tctcaatttc tttttcaaaa tcaactaatt cattaagagg tataaatatt
      241 tcaacaccat caataactac tccaactgca tcttctggaa cattagattt atcttttgct
      301 atctctacat ttgaagcaga tgctagagtt ataaaatagt ctttaccaag ctcaatagct
      361 tctatttttt cttcacttgg gattattatc aatttagctt tcttagaagg tggtacattc
      421 atttctgctc tcacatttct tatacttctt ataccttcca tcaataagtt catcatatct
      481 tcttcttcag ccatgttatc ttcttcatta tattttggcc attcagatac tattatacat
      541 ccttcaacag taggaagatg agtatatatt tcctctgtta taaatggcat gtatggatgt
      601 aataatttta atattttttc aagaacataa gttaatgtat ataatgctgc agatttagca
      661 tttgcatctt ccccatataa tctaggttta accatttcaa tataccagtc acagtattct
      721 gaccaagtaa aatcgtatat cttttgaagt gctattccta aatcaaactt atccatgtta
      781 ttagttgctt cttttacaat attatttgct cttgatatta tccatttatc agctagagtt
      841 aagtcctctt taacactttc tctagtcatg tttttaatta tatcttcatc aatattcata
      901 aatacaaatc ttgatgcatt ccacaactta tttgcaaagt ttctagcaaa ttctactctt
      961 tccatataga atctcatatc atttcctggg gaatttccag ttgttaatgt aaatcttagg
     1021 gcatcagctc catattgctc gattatctcc aatgggtcta taccatttcc taaagattta
     1081 ctcatttttc taccttgaga atctcttact aatccatgaa ccaaaacatg gtcaaatggc
     1141 ttttcattca tacagaacat acctgcaaat gccattctaa ctacccagaa gaatattata
     1201 tcgtatcctg taacaagtac acttgttgga taataataat ctaaagcttc agtcttattt
     1261 ggccatccta gagtagagaa aggccataat gcagaactga accaagtatc taaggcatct
     1321 tcatcttgtt taaaatgagt gcttccacat ttaggacact tatctggcat ttttctagca
     1381 actactattt ctccacactc ttgacaataa taagctggta tttgatgacc ccaccataat
     1441 tgtcttgata tacaccaatc tcttatattt tctagccatt gtaaataagt tttatcaaat
     1501 ttatctggaa caaattctaa ttctcctttt ttaagaatat ctatagctgg ttttgcaagt
     1561 tcttccatct taacaaacca ttgctctgat aatctaggct caactactgt atggcatcta
     1621 taacaagttc ctacattatg attatgcgct tttatcttaa ttaggtaacc ttgttctttt
     1681 aagtctgcta cgatagcttt tctacactca aatctatcca taccttcata ttttccacaa
     1741 acttcattca tagttccatc ttcattcata gtatttaact gctcaagatt atgtcttaat
     1801 cctacttcaa aatcattagg gtcatgtgct ggagtcatct taactgcacc agttccaaac
     1861 tctaaatcaa cataactatc tgcaactatt ggaagttctc ttcctactaa tggaagtatt
     1921 gctgtttttc ctattaaatg tttatatcta tcatcttctg gatttactgc tataccagta
     1981 tctcctatca ttgtctcagg tctagttgta gctatttcta aaaagtcttc actatctttt
     2041 agtgggtact ttatatgata aaagtttccg tcgtgttctt catgttcaac ttctgcatct
     2101 gataaagtag ttttgcaatc tgggcaccag tttattattc tatttcctct gtatatgtga
     2161 cctttttcat ataggcttac aaaaaattct ataactgctt cattacaccc ttcatccata
     2221 gtaaatcttt ctttatccca atcacaagaa tcacctagtt gtttaagctg attagatatt
     2281 tttcctccaa attcatcttt ccattcccag gctctcttta aaaattcttc tctacctatt
     2341 tcatactttg tcttgccttc ttgttttttt attctttcaa caactttaac ttctgttgct
     2401 atagaagcat ggtcagttcc tggaagccaa aaagcttcat atccatccat acgtttccat
     2461 ctgattaata tatcttgaag tgtatggtca agagcgtgtc ccatatgcaa ttgccctgtt
     2521 atatttggtg gtggcatcat tattgtaaat ggttttttat ctggatttgg tttagattta
     2581 aaataccctt catccatcca ttttttatat agcctagcct caaaatcttt tgggttataa
     2641 gtttttgata aattcgtatt ttccattgtc ctgcctcctg tattttataa ataaattttt
     2701 taaatatctt tagaaataaa aaagagctct tcatcctctt taaaaaggac gaaaagctcg
     2761 cgttaccacc ttaattccaa agcttaaaat acattaacat ataattaaat tattagttaa
     2821 tgttatttta tcaactttga ctctcaaaga atttaacggt tctaaccgtc taaacttact
     2881 tatagttcag tttagaaact ccaaagctac attcagtaaa tcttatactt agaaaatctt
     2941 acagccagtg aattttcctc tcttttagta gtatttactt actcctcttc ttctcagtct
     3001 ttaatattac atattattat atacaaatat tattctcttt gtcaattatt tttattttat
     3061 tttttctgaa ttttcttaat tatgtctatt ctcttttata tagttgttat attttaaaat
     3121 atgtttttac agttattctg tcttataatg tcaataattg caagattatt ttattttaat
     3181 tttaatgctt tctcattaat tttatattat attatcctta cagtatacct cttattcgta
     3241 ttttttatat tatatgtttt attttataaa taatttactt tatcttttac tatatttaaa
     3301 tttattataa gttttcttgc cagaaatttt taggtgttat attttctgta attggcaata
     3361 ttgtatatcc tctctctttt aagattctta taatattatt taaagcattt aaactttgct
     3421 ctttctcatg aattaatact actaattcat ctctttttct tccataatat aatatattac
     3481 ttactatctg acttgtagaa gacttccaat cctgtgtatc caaattccag tcccaaatta
     3541 aataatcatt tgccataagt ttattatatg aaccttctgg catatatggt ttacttccgt
     3601 atggtaatct tattaatttt gaactctgac cagttatttc ttgtagagta ttattgcatg
     3661 tattaaattc cccaactgtt acttctgggc ttttatataa cttatgtata tcatgactta
     3721 cactatgaaa acctattgag ttttcctctt ccaagactct ccttacttct tccttgtgat
     3781 ttttcatatt ttgattaagc ataaaaaatg tcgctttgac atcattttct tttaaaatat
     3841 caagtagtgc atttgtatat tttgatggac catcatctat tgttatgtat gctacttttt
     3901 cagaagtagc ccctggacct tttgtttctt cttgagatgc tttgtatatg atttctccta
     3961 aagtattact ttgagaaagc agttccagtg tattttcacc ttttgaactt atattttcaa
     4021 ctggaacatt attaattttt tcctccaata atgttccagt taaaattaaa acagctataa
     4081 gaaatatcct tataaataca tgaaaaacag ccttattatc tttaattttc atcccccata
     4141 tctccctttt ataaaagcat aagtttaaga cttaaaaagt tattttttat atttattctc
     4201 tttcttatta ctttttatat ctctactaaa tattttttta accttttctt cttctttttc
     4261 tcctaaagat tctactcttt tgattcccaa agaagttaaa aattgtaata catttaaatt
     4321 atcaaatgga gtttccatat tcgtactttc actatattca tcaacattct tagtaccttc
     4381 ttccacactt aaaatacgct tcggacctcc tacacagcct cctacacacc ccattccttc
     4441 tataaatgta gcttctattt ctttatttag aactttcttt agaccgtcca tgcactcttt
     4501 tgttccttca aatgcctttt cttcaaattt tatatttttg tcaattcttc tgactgataa
     4561 cttaactgct ttacttacac ctcctgttct agcataaact ctacctgaaa gggacgcctc
     4621 tactctatta ctctcatcca ttattgctgg atttaactct aatgcatcaa atatttcttg
     4681 caactcttta aatgtaagca caaaatcaac tgcatctttt atatcttcta acattgcttc
     4741 tttctttttt gccatacaag gacctatgaa tacaacttta gcatctggat ttaaaatttt
     4801 tacagctctc ccacaagcaa tcataggaga aacggatggt gatacattct ccaatatctg
     4861 aggaaaacta ttttgtatca aactaaccca aataggacag caacaactag ttataaagca
     4921 tccttcttcg tcctttttca tatgctcata ataatcatat gcttcttttg cagtaagcat
     4981 atctgctgcc aaagcaactt ctatcatatc ttcaaatcct atctttttta atgcagttct
     5041 taatttacca gggcttacat ctttcccaaa ctgtcctaca aaagctggtg caactattgc
     5101 atatacaggg ctctttatat cttttaacaa ttttatcatt ggtataaatt ctattttatc
     5161 tgaaattgca cccaacttac atcttgatac acaatcacca caagcactac atacatcttc
     5221 ctcattacaa ttaatatgct tacttcttat cttacatgac tcatcttcct tattattttc
     5281 atcacaatat ttgcaagcat ctgatattac agatattatt ggtcttttga cttcatctaa
     5341 atttacagct tcatgtagtg actcttttaa atttttatct gtatcttctg tcacatccaa
     5401 tcccatagtt accctaatta aatttaccat agtatcttta tcaaattttc cactaaaata
     5461 attatttaaa ttatccttta aatcttcatt taaaatttta ttaggtaagt tttcaatatc
     5521 ctctatatca tcattccaca ctgcttttac aagttcgcca aaaagcttca ttttatatat
     5581 ttctagtatt ttagaattgt catttctagt attcataaaa aatagtacct ccataaatta
     5641 aacttatata tgtttaatat ttacaaagat actatttttt atttattatt taactactat
     5701 tattaatatg atttatttag ctttctcaac aactctattt caatcattat tttcattaga
     5761 tgatagttga atacggggct tttttcttca tctatattcc agtctagctt atccacatta
     5821 tatgttttct taaggtattt atatgtatgc tctttcaaat aaagtttatt ttcaagtaac
     5881 tctatagact gcatatgaaa atgtatttct ctacatatcc ttaaggcatt ctcaattttt
     5941 tctatattag tttcactcaa actataatcc aactcatcaa taagttttga atatatttct
     6001 tctaattttt ctacttcttc ttcaaatttc tctacattta aattttcttt tctaagaatt
     6061 ttatcttcaa taagtaacat tgaaagttct tctatctttt taaattcatc tataacacta
     6121 tttagataat ttggtctagc cacataataa ttaacaagta ctccaaacaa aacacctaca
     6181 gatgtatcta acactctatg taaagaataa tacacagggt cactatttcc tacacctaga
     6241 tgtatagcca gaaaggttac acaggcaact actactgact ttattttaaa cagattgcat
     6301 aaatatattg agcacattac tcctagacca cataaaaaag ggtctccagc tttaatgatg
     6361 gcaaatatga atcctattat accacctaaa atagtacctt ccactctatt gattcctgtc
     6421 tttaaagaac cttttaccgt atcttgaaca gaaattatac atgctactag agaataaaac
     6481 atattctcta ctagatagtt accaagaatc atacataaag tgacactaat tcctgttttt
     6541 atagtcctca tacccatctt ttgaagtttc aaagttccac ctcctaaaaa gcaccttata
     6601 taaaatacgc atcctcatat ttaatatgtt cctagtatat cacaattttt taaatttacc
     6661 cattaatcat atttttttat caattctttc ttaatttaga caataaaaaa actgctttaa
     6721 agcagttttt tatcctatct caaccatttt accctcaatt gcaaaaatta tcttctcaca
     6781 tacatttgtt atatggtctc caattctttc aaggtatctt cctataagta gaagttttac
     6841 tccttggtct atagtatttg aattttcatg cattattttt aaacaatcaa tttgtacttg
     6901 ttcatacaat ccatctacaa tatcatcttt agttccaaca tcataagcta tacttgcatc
     6961 aaataagatt aaagcttctc tagtctgaga catcatagac ttacattctt ttgccatctt
     7021 aggaatatct atcagctctt ttatataagc ctctccacct atcttcaaca tttctctagc
     7081 tatatttgct gcataatctc ctattctttc caactctatc gatatattac ctaatgaata
     7141 aataaatctt aagtcccttg ccattggttg ctttaatact attagctcta tactcttatc
     7201 tcttatttct tctcttaatt tatttatttc ttcatcatct tctactactt ttcttgcacc
     7261 ctctaaatcc ttctctatca tacaagtcac agacttttct attgcttctt cacatttttc
     7321 catcataagt actgtttgtt cttttaaagt atcaatactt atatctaaac tattattgac
     7381 cattgtctaa ctcctttata gttatatatt ttaaccaaat ctacctgtta tatagtcttc
     7441 tgttctttta tctacaggag tggtaaacat agtttttgta tcgctaaatt ctataacctc
     7501 tccatttaag aaaaaagctg tttcatctga tatacgagca gcttgttgca tattatgtgt
     7561 aactatgact attgtgtagt ctttttttaa atcctctata agttcctcta cttttaatgt
     7621 tgaaattggg tctaaagcag atgtaggttc atccataaga ataacttctg gtctcatagc
     7681 aattgctctt gctatacata tacgctgttg ttgaccacca gaaagtccta gtgcagatga
     7741 tttaagcctg tctttaacct catcccaaat agctgcacct tttaagcttt cttctacaat
     7801 tttatctaat tgcttcttat ctcttagtcc atgtgtacga ggaccatatg ccacattatc
     7861 atatatactc ataggaaatg gatttggttt ttgaaatacc attcctactt tagtacgaag
     7921 atttataaca tcatctgagg tatatatgtc ctctccatca acagaaatgt ttcctttaat
     7981 tgttacatct tcgattaagt cattcattct atttaacgtt cttataaaag ttgattttcc
     8041 gcatccagat ggacctatta aagcagtaac cttattctct tttatatcca tatttatttt
     8101 ctttaatgct tgcttatcac cataaaataa atccaaatct tttacactca tttttatctt
     8161 atcgattaat tccattattc acacctctta aatttaataa ttagctttat ttaatttctt
     8221 agcaacaaat cttgctagca tgtttagtgc taatacaatt actattaaaa caattcctat
     8281 actggctgct gttgctatat ctccactctc tttagtcagt aagtatgagt gtacagttaa
     8341 tgttcttgca ctatcaaata ttccacctgg catcttggct acagtacctg ctgtaagtaa
     8401 tattgcagct gattctccaa ttatacgacc aactgataag attactccag ataagattcc
     8461 tggtattgca cttggaagta ctaccttata taaagtttga aattttgtag ctcctaatgc
     8521 caatgatgat tctctataag cttgaggaac tgtttttaaa gcttcttctg ttgtacgaat
     8581 tattactggt aatattatta ttgcaactgt aagtgaacct gcaactatgg aatatcccat
     8641 ttttaatgta actacaaaga aaattcctcc aaataatcca tatactatag atggtatccc
     8701 tgataaactt tcagttgcaa atcttatagc tgtaactgct tttccttttt tagcatattc
     8761 ttgtaaatat atagcagcca atattcctat aggagttgct acaactatag aaactactac
     8821 tgtatataat gttgtaatta tcattggaag tataccacca tcacctgatg ctgaataatt
     8881 gctaaataag aaattaaaat tcatgttgcc aattcccttt ataaatataa atcccactat
     8941 aatgactaat gatactattg taaatagtgc tgatagatat actaatgact tcaatagatt
     9001 ctctttgaac tttctcatct taatttaccg ccttatttga aagtttattt aaaactaagt
     9061 ttaaaattaa tataaatgta aacaatacta ctccagttgc aaataacatt tcttgatgtg
     9121 taccaaatgc gtatcccatt tcaagtgcta tattagttgt aagtggtctt acactatctg
     9181 ttattgcagt tggcataact ggtgagtttc ctgcaacaag tattactgcc attgtttcac
     9241 ctaaagctct tcctactcct aatactactg cagctaatac tccagatttc gcagcaggta
     9301 gtacaacttt gaatattgtt tctatttttg atgcaccaag tgctaatgaa ccttctttgt
     9361 atgcttttgg tactgctctt attgctgttt ctgatacaga tattattgtt ggtaacatca
     9421 ttactgctaa tactattact attgctaaca aactttgacc ttttggaaga ttaaatatac
     9481 tttgtatagt tggaactatc actgcaagac caaatacacc atataatact gaaggtatac
     9541 ctgctaaaag ttcaactgct ggtgacatta tttttgctaa tcttttaggt gctacttctg
     9601 ctataaatac agaggttaat ataccaacag gtactcctat cacaagtgct ccaagtgttg
     9661 caaatattga tgctactatc atagatgata ttccaaattt attggcagaa ggaacccaat
     9721 ctgcaccagt taaaaagtct ataaatgaat atccttcaaa tataaatggt cttaagcctt
     9781 tataaaatac gaatcctatt atcaaaagta aacttgcaac tgctactaaa gcactaagta
     9841 gaaaaatatt ttttgctact ttttcaatta cgtattttgt cttattgcca ttattgtcat
     9901 ttttttctaa acctactaat ctagatttta ctatcataat accctcccat taaaatatac
     9961 ttatcgattt tcgttctgta ttcaaaattt aattttttat aattcgtctt catcaaattt
    10021 cctgtacttt tattctactt tttttaagtt aaatctatat taggattaag ttaagcaagt
    10081 gtaaaattat atttactttt ttaacattaa ttttactttt aaaactatat ttgacattta
    10141 ctttacaata tactgttgtt tctctatatt aaaatagcag atatagataa taatacagtt
    10201 tatgtataat ttattaccta tatctgctat taagatttgt taactaaatt taacttttat
    10261 actacatata taattatcgt atcctctagt ttatatcact attatttcta tcaaattgtt
    10321 atgatacttg atgtttgcaa taggtacgaa tagccaaaaa tataaaaata ggtatttcta
    10381 tcactgagaa aaacagtata atgtgtggtg tccaaaaact catacctcct aagtgtaaga
    10441 aattaaatga tgtcatttga tataataaac taatcttaag accaattccc cctggtggta
    10501 ataggtatcc aatccaacta acacctattc ccataggaat taagcaaata gaaaaagcaa
    10561 tcagcataga agtaagagaa tccttacatt ttgcagataa aaataatgtt aaactaacca
    10621 ttgcaagtat tgaaattaga ccaccaatag ctataagaat ctgtgcttgt agtaaattta
    10681 tatttactag actaatgaca gaaaacaaca tttgaaatga ggttttcaaa cattcagtac
    10741 caaaagctaa atcagaaatt gccaaatgga tagaaataca tacaacaaac attactataa
    10801 atatagaaaa tagagctaat attttagtaa acgctagacg agtatgtcca tgtttcgtac
    10861 aacgaaggat gctatctgat tgtgtctgat actcaccaga aaaagtaggt gcagtaattg
    10921 caacacatat aattaataat ataaaaatgt aaaatattat ataatcgaaa gcatctttag
    10981 aataacctgg gtatatctca aatggagtat ctacttttct ataaagttct tgagctttca
    11041 tttgtgcact ggaagtttgt cgttcaatct tcataatatc atttaaatga gttttgcatt
    11101 gttcataaaa agaatctaaa tcatttgtat ttatttctga taattctgtt gcaaatcctg
    11161 tttctgaatt agcaaatgct tcaggaagtc tattcaacaa tgggcgtatt ggtaaaatac
    11221 ttttagaata aatatctagt gggaaatctt cactacctac tttaccatac ttttcttctg
    11281 tttttttcca tatatttaaa gcatgcttta atttttttgg tgttacaact ccactactaa
    11341 tttcactatt attagcataa attgtttttt tatatttaat agcatctaaa ccactcaatt
    11401 caactacctt gccattatta tctaaataat tgataccctc gtaagtaatt ggaagaaaag
    11461 ccatcaatgc agataaaacc aatgccaaca ataataaaat taatgtacga cgtgagtgca
    11521 acactcgttt aatttccaaa taaaaaatac gcatttattt cccctcctta ctataaactg
    11581 cttctggaaa taaccataaa tataaatctt ctagtcgtgg ttcaacagca atagagttat
    11641 caatttgtga cttttctgcc aagtaacgaa tagatacttg attgtttttt tcactacgct
    11701 gattgattat acgtaaattc atttcatatt ttgaaatatc tctatttgat ataagacatt
    11761 cccatacttt gccctctact ttcttaacca gtttatcggt tgttcccaca tcaagaattt
    11821 ttccactttt cataatagca ttttgagttg atatgtactc aatatcagat acaatgtgag
    11881 tagatattag gacaacacga ttatgagaaa attctgaaat aaaattacga aatctcactc
    11941 gctcaccagg gtcaagccct gctgttggtt catccattat aaggatttta gggtcattta
    12001 acatggcttg tgcaattccc actcgtcgtt tcattccacc agataattta ataatctttt
    12061 tattttttac atcagataat gtaagaattt ccaatagatt attaattttt ccttttactt
    12121 ctcttggtgg tacatctttc aatgctgcta tatattctaa ataatctttt acagaaaaat
    12181 cctgtgggaa accaaattct tgtggtaaaa atcccagtaa atttctgtac tcagcaccca
    12241 ttatataaat attttttcca ttatacagaa ttttgccatt ggtaggtttc ataatgtctg
    12301 taataattct catcaaggta gttttacctg ctccgtttgc tcctagaaat ccccacacac
    12361 ctggtgttaa ggtaaggcaa acatcttcta cagcaatttt atccttaaat ttttttgtca
    12421 catgttcaat ttttaattcc atatttgaaa ctcctctctt tatctactta cctgcaaaaa
    12481 caaaagtgtc ttgcttagta tttattttac taaacaaaga cactcataat tttaatatta
    12541 aatagataat ttcttaagaa aatcctaata tttgatttat attttactaa atagatgtag
    12601 gaagcgatat agtaaatgtt gtgacttcat ctttactttg aacagtaata tcaccaccat
    12661 gcaaatttat aatttcctta gctatagaaa gccctaaacc tgcaccaccc atatcagatt
    12721 ttctagcttc atctaaacga ttgaactttt caaatatagc agaaagttgt tcttctggaa
    12781 ttgtctgtcc ataatttcta aaagcaatat ctaccttgct gtctttatga accgctgaga
    12841 taataatttc tgtattagga tagctgtaag caatagcatt tttcaagata ttattaaaca
    12901 ctcttgcaag tttttcagaa tctgctttta ttgttaaact ttcatttgct tttagaatgg
    12961 ctctatttcc tttttcagac agcaatgggt aaaattcatc ctttaattga agtaacatat
    13021 aatataggtc tactttttcg aaattaattt tgatttgatt agaattatat cgtgttattt
    13081 caaaaagttc attgataagt ttttctaaat attgtgcttt gtcaagtgca acttcagtat
    13141 acttagattt ttgagaatta tccatattag gtatttcttt taataagctc aaatacccaa
    13201 taactgaagt aagtggtgtc cttatatcat gtgctagata catcacaagg ttattctttt
    13261 tattttcagc ctctttcact gcttgttgac ttaacaaaac agacatctta atttggttca
    13321 tttgttgctc tatttccttt aatggcttag atagttcaac caattcatca ttttgttgat
    13381 aaatagtatg tgttgcattg atgatttctc ttaaataatt ccatggtttt ttccaataaa
    13441 aattgaaaat agtgatatat ccaattataa aaaccaaaaa aaataataca tctcttctca
    13501 acttaagcca tttaacaaga ggatttgaaa aatttgaaaa cgtataatca gccataaaca
    13561 ttaaaaccaa tactatacct gtatagatag ctaacattat atataattta tactttaaat
    13621 acgcctctgt atttttttct ctttttataa atttatattt atctcttagt ttcctataag
    13681 taaaattatt aatctttttc ataatactac atcccttctt gacaagaaaa ataggtgtca
    13741 aatcttgtaa ccaacacccc aaattgtttt tatatatttt gcttcatcta cagtatcacc
    13801 taatttttca cgaagatggc gaatatgaac tgttatagta ttgttacttt tattgtaata
    13861 ttcatctttc cagatagcat gaaaaagatt ttctgaattt accacagagc ctttattttt
    13921 caataataca tgtagaatag aaaactctgt tggagtaagt gaaagagctt taccatttaa
    13981 atggcattca tatgtttggg tattcaattc cagacctaaa aaagaaaaaa tatgtgaaag
    14041 ttcttcacca gattgtaaac ttccactgta tttcttatat cttctaatct gtgccttcac
    14101 tctagcaaca agttccaaag gtcggaaagg tttagtaaca taatcatctg cacctaaagt
    14161 taagcctgta atcttgtctg tttcttcatc cttagctgtt aacataatga ttggataagt
    14221 atatttttct cggatttttt ggcataaaga aaatccattt actcctggca acataacatc
    14281 taaaattgca aggtcaaatt catttaaatt aatgcagttt agtgcatcaa gaccagaata
    14341 aaatttataa acaatatagt tttcatttgt aaggtagact tctattaaat cagctatatc
    14401 acgttcgtca tctacaacta aaatttttcc actcatagac tttaaacatc ctttctatat
    14461 atatttatat atattatact aaatttaatt tttttatatg attttttaca ttagaattta
    14521 ttaagaaatt cttaatctta ttatgtaact attttattat ttaacatata ttaaagataa
    14581 aaatgaatat atatagattt agatatacaa tttatctatc ttttcaaata taatctataa
    14641 aataatagtt ctgttacgca ttttttcata taataa
//
LOCUS       DAEPLF010000074        14661 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEPLF010000074 DAEPLF010000000
VERSION     DAEPLF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14661
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.074"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            108..1502
                     /gene="yfcC"
                     /locus_tag="KR314_001968"
     CDS             108..1502
                     /gene="yfcC"
                     /locus_tag="KR314_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBH0328351.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            2019..3386
                     /locus_tag="KR314_001969"
     CDS             2019..3386
                     /locus_tag="KR314_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBH0328352.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            3411..4019
                     /locus_tag="KR314_001970"
     CDS             3411..4019
                     /locus_tag="KR314_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBH0328353.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            4067..4438
                     /locus_tag="KR314_001971"
     CDS             4067..4438
                     /locus_tag="KR314_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBH0328354.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            4617..4955
                     /locus_tag="KR314_001972"
     CDS             4617..4955
                     /locus_tag="KR314_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBH0328355.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            5422..5925
                     /locus_tag="KR314_001973"
     CDS             5422..5925
                     /locus_tag="KR314_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBH0328356.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            5972..6931
                     /gene="moaA"
                     /locus_tag="KR314_001974"
     CDS             5972..6931
                     /gene="moaA"
                     /locus_tag="KR314_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBH0328357.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            6935..7405
                     /gene="moaC"
                     /locus_tag="KR314_001975"
     CDS             6935..7405
                     /gene="moaC"
                     /locus_tag="KR314_001975"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBH0328358.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDYMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            7441..7872
                     /locus_tag="KR314_001976"
     CDS             7441..7872
                     /locus_tag="KR314_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBH0328359.1"
                     /translation="MAKVVSINISEKKGVIKVPVPCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
     misc_binding    8303..8514
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8677..9966
                     /locus_tag="KR314_001977"
     CDS             8677..9966
                     /locus_tag="KR314_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBH0328360.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            10020..11099
                     /locus_tag="KR314_001978"
     CDS             10020..11099
                     /locus_tag="KR314_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBH0328361.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            11120..12664
                     /locus_tag="KR314_001979"
     CDS             11120..12664
                     /locus_tag="KR314_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBH0328362.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(12827..13369)
                     /locus_tag="KR314_001980"
     CDS             complement(12827..13369)
                     /locus_tag="KR314_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBH0328363.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDNKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(13517..13801)
                     /locus_tag="KR314_001981"
     CDS             complement(13517..13801)
                     /locus_tag="KR314_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0328364.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(13864..14628)
                     /locus_tag="KR314_001982"
     CDS             complement(13864..14628)
                     /locus_tag="KR314_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBH0328365.1"
                     /translation="MNNYLKFATYQLIGFTSSIPSLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         5551 a         1531 c         2593 g         4986 t
ORIGIN      
        1 ttatatcttt gctaataaaa aattgtaaaa agggaaaact ttagtcagag agattaaaca
       61 atatcctaga aagatttaaa atttagtaaa gtatggagga cattataatg actgacagta
      121 aaaagaaacg atttattata ccacacactt ttacaataat atttttacta atagtcttga
      181 tggcaatatt gacttggata gtaccaagtg gtgaatttag tagagaagat gtagaaggta
      241 gaatggtagt tgtaccaggg acttatcaaa gtgtagaaag taatcctcaa ggatttgcag
      301 atgtttttaa agcaccaata gaaggcttta tagattcagc tgaagttgta ggttttgttt
      361 taatagttgg aggtgctttt ggtattgtaa ataggactgg agctatagaa gcaggaatag
      421 cttcggttat ctctaagttt aagggaaaag aacttcttat aattccaata tcaatgattt
      481 tatttggact tggaggtaca acatttggga tggcagaaga gacattacca ttttatatga
      541 tatttgttcc actcatgaca tccttgggat atgattctct aacagcaata gcaattgtat
      601 ttataggagc agcagcagga gccgccgctt caacaataaa tccattttca gttggtattg
      661 cacaggcact agctcaactt gctcctggtt caggagtaac ttacagagta gttattttta
      721 tagcatatat actaataagt atagcatttg ttatgtacta tgctaagaag gtaaaggaaa
      781 atcctgaaag ttcaatagta tatgaaatag ataaaaataa taaatcaata cttaatcaaa
      841 cttcaacaga aataaaagaa tttactttta aagaagtatc tgttcttgct atatttggaa
      901 tagggatggc aataatgatt tggggtgttt taagtcaagg atggtacatt ccagaaatat
      961 caatgtgttt tgctgcaata ggtgtgcttt ctggattggt tggtagatta tctcaaattg
     1021 aaatctcaga aagttttata gatggagcaa aagatttagc atctgcagct cttgctatag
     1081 ctttggctcg tggaatcgta ataattgctc aaaatggatt tataatagat actatattaa
     1141 attccgcagc agcctttcta ggtgggcttc caaaggttat atttgtatat ttatcatttt
     1201 tcttggaagc tgcattggca tttttaattc catcatcttc aggtcttgct tcacttacag
     1261 tacctgtact tgctcctctt ggtgatttgg ttaatatatc ggctcaagtg atagtaaccg
     1321 cttatcaatt tggtgtgggt cttacaaact ttataactcc aacttcagga gtactgatgg
     1381 gagcacttgc agtagcccat attccatttt caaaatgggt aagatttata atgcctatct
     1441 tgttgatttt gacagctgta tctctagtct ttttaacaat aggtatttat atagggtttt
     1501 aattaagtgt agttttattt aagatattta gtaatggtat aaaatgataa tatgttttca
     1561 ataaattgcc actaatttaa ttagtggtaa tttatttttt gtatttaaaa ataaatgtat
     1621 ttacttgtat tttgaattaa tatctactta gacattgttt taattgttat attttcattt
     1681 aattaagcat tgatttatcg ttttttatat ttcatataac aatgcaaaaa ataattttta
     1741 tatagtaaat gacaaaagtt tacaataaat gtatattttc ttactcattc cttaaaatta
     1801 ctcatttagt ttaataactc tcatttcata tgtaaagttg gtaaaaaaat taaaattaaa
     1861 agtaaaaaac taaataaatt aatgttaatt tgaaatatat atgaaggttt ttggagaata
     1921 cttacttatg ataggaaaaa atgttaaaat aatagtgtaa tcaaatataa aggactgaaa
     1981 taattaacag ttattaaata ttaggagtgt aaaaatttat gggattaaaa gaatttgatt
     2041 ttattgaaga gtctattgat atgttgagga caatgtcacc aacattagaa actatatcag
     2101 atgaaataga agagtatttt gaaaatatct tggatgaaaa gaatcaagaa tatataaatg
     2161 taacctctag aataaaatca gaatcaagtt taagagaaaa gataattaga aatagatatt
     2221 taaaaaaata tggagaagca agtaatctta ttcataatgt ttctgactta ataggcctaa
     2281 gaattgagtg tagatttatt gaggatgaaa acaaaatata tagacttttg agaagatatt
     2341 ttaataaaac agatgacaaa ataaactatt ataataaaga gaataagaat attaaattaa
     2401 aactatctga gaggcaaccc aataagcaaa aaaatggatt tgaaatatat aaaatagatg
     2461 gagtttttgc gtatttagat agagaagtaa aatttgaact tcaaattaaa tctcttgtaa
     2521 atgtgttttg gagtgaaata gaacataaaa taatttataa aaataataca tatttacttg
     2581 ctgataaatt tataaaggat atgatggact ctataaagaa taatcttact atgatagata
     2641 atcagctttt aagtatttat aaaaactttc attcagggaa gtcttttaat atgaaagtaa
     2701 gtaaaaaaga aattgaaaaa ttatttgcga aactagtcta tgacgctttt tctgaaaaga
     2761 tgaataaaag tataggtttt gttgtagatt ttaaaaaacc atgtgaaact atattgaact
     2821 attcttttaa taaacaagat ataagtgatg aagttttagg taattttatg cttgatgagt
     2881 ttgcaagatt aaatgaaatt gtaaacaagg atatagattt taatgagcaa atagaatttg
     2941 aaagagaacc aacatttgat gataagtttt gtaaagacct aggaaatcat tttagaagta
     3001 gattaaacac agaatttcca tggaacctat tttttagaat attatttgaa atagagccat
     3061 ataacaatac caaagacttt gaaaactttg tagagtttat aaaagaaaat gtgctactag
     3121 aagaaaatag ggagcaatta ttatgccaat ttgaagaaga tagcaagctt ataattgaag
     3181 atatatatga ttgtatattt aatagtataa gtgagattga tagtgttgaa attttatata
     3241 actataatct tgaaaaaata aactttacat caagtgaaat cataaattgt atatgtagag
     3301 aatatgagtg ttatggtgaa tacttagaag aaaaagaaaa gcttatgaat acatttaaag
     3361 aaaagattat tcaaatcttt gagtaatagg tcaatgctga agtggcgcta atgcgccatt
     3421 tttcaaatat atatgaatta agtaaaatta ttttggagga ggcgatttgt atagagaaaa
     3481 caggtgtaat tttggcaggg ggaaaaaatt ctagaatggg tagagataaa gcctttttag
     3541 agttacatga taagcttttt attgaaattg caatagaagc atttaaaaac tttgatgagt
     3601 tgataataat atctaataat gaagaattat attctaaata cgatattaaa gtttataatg
     3661 atatagtaaa agatgttggt ccaattggag gaatatatac tgctcttgaa tatgcaaagt
     3721 atgatatagt tacaattgca tgtgatatgc catatctaaa taatcaaata gtcgaaagaa
     3781 tagctaataa aatgaatgat aaaagtgtga tttcagtgac aaatggaaag ttacaaccat
     3841 tgtgttctgg atataaaaag agtataataa ataaagtatc tttatgtata agggaaaatg
     3901 atttaaagct cagaagcttt atagataaaa tagataaaag ttatatatac tttgatgaag
     3961 aagatttatt tttaaatgta aacactgttg atgagtatga aaaattaact aaagtttaat
     4021 tagctatggt tgtaaaatag agtaggattt atatggagga gagtttatga atttacttaa
     4081 atgcgattct tgggcagtaa tattaaataa tagcgataaa gaaagtaaag catataaaat
     4141 attagatgaa cttaaaagga atatgtacaa agttgttgct atagatgaag agaaaaaacc
     4201 tattgaagga atagatgtgt atgaatgttt aaaagatgtt ccacacaata ttgatgttgt
     4261 agctattatt gataaacaat ctaagatgga tgtgatttta gaagaagtag agcttttgga
     4321 tatacaaaat atgtggtttg aaaaaggaag ctttagtgaa atgatgatta aaaagactaa
     4381 agatttaaaa ttaaatatag aatataattt aagtttatat gatgagttga ctagataaaa
     4441 ttatatttct taataataaa aatataatta gttaataaaa catattattt tatagtatag
     4501 attggttaat aaaagtacag attggttaat gaaacattaa attacaatat ttgttaaaag
     4561 aattgtgagt tacatgatat aatgtattaa ataggatact aaggagttga tatattatgt
     4621 taatagtaac tacagaaaaa gtagaaggta aaaagatatc aaaggtttta ggattagtga
     4681 gaggaagtac aataagagca aaacatgttg gaaaagatat aggagcaagt tttaagaatc
     4741 ttgttggagg agaacttact ggatataatg aaatgctcac tgaagcaaga caaattgcca
     4801 taggtagaat ggttgaagat gcagaagcta aaggtgcaaa cgcagtaata gcatttagac
     4861 tgtcttcagc ttcagttatg caaggggcag cagaaatgct tgcttatgga acagcagttg
     4921 ttttagaaga tgataatagt attcttgaaa aataaaatat aaatagttaa aaagaggcat
     4981 cttttaaaaa ttttatggat gccttttttt atttgaaaaa actaatttga gaagaaattt
     5041 taactattat gacaataaaa ttagggtgat attatataaa taagtaaaat tatattatat
     5101 aatgtatggt atagacttta aaaattttat ttgtagagaa aaatatggac aatgttataa
     5161 taattataga aattaaaagt acttacattc attatgtatg tgacatgtat aattagtttt
     5221 atatttaata cagttttgaa gtaatatctt cattttgaga acgttggaat aataaaaaat
     5281 gtatgtatta taggtgatat aaataaaaaa taaatataat atgttatatt gctgatagaa
     5341 aagagaataa aagaaaatag gttttattaa aagagatttt tatttatagg acttaatcaa
     5401 tgtatagagg ggtgagaaca tatgtttaat gttgcaatta taacacttag tgataaagga
     5461 tatgaaggaa aaagagaaga tataacagga aaaaagttaa ctgaatttgt tgaaaataca
     5521 ggagcttata aagttactga gtatgtactt ataaaagatg ataaggaaat gctaaaggaa
     5581 aatattataa gactttgtaa tagtaataaa atagatttaa ttcttacaaa tggaggtact
     5641 ggtttttcaa aaagagatat aaccccagaa gcaactaagg aagtgttgga aaaagaaata
     5701 ccaggtctga gcgaatatat gagaatgaaa tccacagaaa ttactaaaaa ggccatacta
     5761 agtaggggtg tgagtggaat aagaaataat tctataataa taaatttacc tggaagtcct
     5821 aaaggtgcag ttgaaaatct aagttttata attgatgttt tagaccatgg catagaagtt
     5881 ttaagaggag aagctacaga atgtgccact aaaaaaagtg aatagtatat aatctagata
     5941 gataaaaaat agacaaagag gtagataaag tatgattgat aagtatggaa gaaaaataga
     6001 ctaccttcgt atttcactta cagataagtg taatttaagg tgtgtctatt gtatgccacc
     6061 agatgtaaaa tttgataaaa attatataaa tgaaaatcta agctttgaag actacaagtt
     6121 tattataaag gctatggctg aacaaggtat aacaaaaatt agatttactg gaggagaacc
     6181 tcttttatat cctaaattaa atgagcttat aaaatttaca aaagaagaat gtggaataaa
     6241 taacatagga atgactacta atgctattgg gctttctgat agaatttttg agcttgaaaa
     6301 atgtggctta aataaagtta atataagttt agattctttg aaggaatata agtataaatc
     6361 cgtaacaaga ggaggaaact taaaagatgt cttaaaatcg attgaatctt gtttaaactt
     6421 aggtataaaa gtaaagttaa attgcgttgc tataagtggg tttaatgatg atgagcttaa
     6481 agattttatg tttttaacag ttaaatcacc aattgatgta agatttatag agcttatgcc
     6541 attaggtgag gctaatagag tttataagaa gggttatttt aatgtaaaag aaatgattga
     6601 aaaaatatat ggtttatata aagtcaaaaa tgatgaaaaa agtacagcag aatattatat
     6661 gtttaaagga gctaaaggaa gaataggaat tataactcca ttaagctgtt cattttgtaa
     6721 cacttgtaat cgtattagac ttacttctag tggtgcaata aaattatgtc tacattcaaa
     6781 agaagaaata gatataaaac catttttaca taaaccattg ttatttagag aatctatgaa
     6841 agatataata aaacaaaaac cagaaaaaca tcatttaata gaaaataaat gtagtgatac
     6901 aaataggcgt atgtatgaaa ttggaggata agtaatgtta acacatataa atgaaaaagg
     6961 gtatgccaag atggtggaca taagtgaaaa agatgatact aaaagaacgg ccgtagctac
     7021 agctacaatt actcttagta aggaagcatt agaaaaagta aaaaatggtc agataaaaaa
     7081 aggagacgta ttgtctgtag ctcaagttgc aggtataatg ggagctaaaa atacatcttc
     7141 aaacatacca atgtgccatc agattaaatt agttggatgt gatttagaat ttgatgtaga
     7201 tgattatatg tctgctatta agatatacgc aacctgtaaa tctcttggga aaactggagt
     7261 agaaatggaa gctttaaatg catgttcttt agcagcactt acaatttatg atatgtgtaa
     7321 agcagttgat aaaaaaatgg ttataagtaa tatccatctt gtggagaaga ctggtggaaa
     7381 atcaggagat tttaagttcg agtaaattaa taatatattg ggttaaaatt tggaggaaat
     7441 atggcgaagg tagtgtcaat aaatataagt gagaaaaagg gtgtaattaa agttcctgta
     7501 ccatgtgcag agctaaaaat aaatcatggg atagttggag atgcacatgc aggaaactgg
     7561 catcgtcaga taagcttact tggaaaagaa agcatagata aaatgaaaat gaaaggcttt
     7621 gaccaattaa aatatggaga ttttgctgaa aatgtaacta cagaagggat agaagttttt
     7681 tccctgcctg ttggtacaaa gttaagaata gggcaatgtg aagttgaagt aactcaaata
     7741 gggaaaaaat gtcacaatgg atgtgaaatt aagaaattga caggtgactg tgtgatgcca
     7801 cgagaaggaa tttttgtaaa ggttctaaaa gaaggaatta taaatgtgaa tgattcaata
     7861 gatgtaatct aatgaagtca ctattaaaat ataattataa gtaaatacta ttaagattta
     7921 aatatatatt ttaagagaga attaatttaa ataaaatatt ttagttataa attggaatat
     7981 tggttatata aagctatgaa tgtaaaatca tagctttttg tttttgaatt ttaaaatata
     8041 atattttatt tatatatatt aaattatatt ttatataaaa tcaattgttt aaatatatat
     8101 tatttatact gtaaatttat agataataaa gtataaaaaa aatatatttt gatgaaattt
     8161 aaaaaaatac attatataaa tatagtaata gtatgaaaat ttacatataa aactaaaaaa
     8221 tgtaagtaat tgacttttta agttgacaaa atattgcata tatattatga tataaatcat
     8281 aaattaacta caaatcaata ttatatattc gtagaagaag aaagtagcat aaaggaattt
     8341 tacagagagt tagatttggc tgagaactaa tattggaatt tatgtgaaaa ggactttgga
     8401 gaatactatt gtaattatgt tagaatagtg gtttaattcg ccttaagaaa atgagtgagt
     8461 aaaattaatt tttactaata agagtggtaa cacggattta tattcgtctc ttggattttt
     8521 ccaagaggcg ttttttatta taaacttatt tcagaaaatt taaaactata tattaaatga
     8581 aaattataaa aatatgatag ttaataaatt tataaattag aaaattgtaa aatatgctaa
     8641 ggtcagtagt tttacgattt aaatggaggg atgaaaatgg aagaagaaaa aattaactat
     8701 gctctggaac agattccaga gaatcaaaaa cgtggttggg tagctatgtt ttcagtttta
     8761 gtagcaatag gtgtggattt atcatctgtt attttaggcg ctgagttggc acagagtatg
     8821 ccaatgaaac aagcaatatt gtcagtaatt gttggttcat ttttttctgc aattttgtat
     8881 actacatgct ctttggttgg ttcatctaca agtttatcta catctatgat aacaaagtat
     8941 gtatttggag aagctggagc aaaaatattt tctctagtta taggggtttc tctattaggt
     9001 tggtttggag ttcaagtagg tttctttgca caaaatgcac agataataat aaaagatata
     9061 tttaatttag atgtcagtat gcagatttta agcctaatag gtggtctctt gatgatgagt
     9121 actgcaattt atggatataa ggccatggag aagttgagtg tgtattcagt accattttta
     9181 ttagttctta tgatgctaac tatattctta gcttttagag caaatggaat ttcagttgat
     9241 gataatatga aatctactat gacatttgca ggtggagttt ctctgtcaat gagtataata
     9301 atagttggtg caattgtatc tcctgatata agtcgttggg ctaaaagtag aagagactgt
     9361 gctctttcaa gctttttggg aatacaattt ggaaatgctt ttatgataat agtttcgata
     9421 gtattagtaa aatgtatggg aacttcagat attatgagaa tttttattac attaggaatt
     9481 gctataccag gtataatagt acttacatta gcacaatgga ctacaaatac aagtaatgta
     9541 tattcagcat cattaagtat agctcttgta cttaagaagg ctcctgagaa agtattaact
     9601 atagtccttg gtattatagc aacattattg gctgtatttg gaatatatga aggttttata
     9661 ggatttttaa atttacttgg aattgtaatt gcacctgttg gtggtgtata tacagcagaa
     9721 tattacatag taaagcaaga acttaaaggc tttgataaag gagtattata taaaccaata
     9781 gttaaacgtt caatagtgtc ttggataatt ggtatattaa taacttacct ttcaacatat
     9841 ggatttataa ctcttactac tatagcacct ttagatggtt ttatagcagg gtttgtagta
     9901 caatctatta ttggaaaagt gctttgttca acaaaaaata agcaagaaat agacaaagca
     9961 gtataaagaa atttgtttat aaagtataaa ataaattaga aatatgagga gaaagtaaca
    10021 tgagattttt aacagaagaa agtgtagata aaatagcagt tggtgcagca gtgcttggaa
    10081 ctggaggtgg aggtgaccca tatgtaggta aattggttgc aaaacaagcc attaaaaagt
    10141 atggtcctgt aaaagtaata agtttagatg aacttgatga tgatgctttg gtagttccag
    10201 tatctggtat gggttctcct gtaattacaa tagaaaaatt actttcagaa gtagagttaa
    10261 ctaccccttt agaaataatg gagaaattat taaatagaaa agtggatgtt ataattccta
    10321 tagaaatagg agggataaac tcacttatgc ctatagcagt tgctgctaaa aaaggacttc
    10381 ctatcttaga tgcagattct atgggtagag ctttccctga agctcaaatg gtaacttttt
    10441 atcttgaagg atatgaggca tctcctgccg taatgtctga tgaaaaaggt aattctgcca
    10501 tcctttatcc tgtggatgga atttggtcag aaagattagc tagaacttta actgtagaaa
    10561 tgggaggaag ttcttctatt agtgactata atttaagtgg agctcaagtt aaaaaagctg
    10621 ctataggtaa tactcttaca atagctgaaa ctataggagg gtttttactt gaaaataaat
    10681 ctgattcaaa aggtgctgtt gagaatatac taaaagaatt gtgtggatac aagttatttg
    10741 aaggaaaagt aattgatatt aagagagagt tgaaaggtgg atttactaga ggacatgcct
    10801 tttttgctgg gattaatgag tatgatggag agtattctat attattccaa aatgagaatt
    10861 taattgccaa aaaaggtgat acaccacttt gtattacacc agatttaata gcagtattag
    10921 atttagaaac tggatttcca ataactacag agagaataaa atatggctct cgtgttatgg
    10981 tagtagcttt tccttgtaat gaaaagtgga gaacagaaaa aggaattgag acagttggac
    11041 ctggatactt tggatatgat gtggaatata aaacagtaga agaattacaa ggaaaataaa
    11101 agtttaggag gaaattttga tgtatcgtgt aggaattgac gttggaggaa caaatacaga
    11161 tgcttgtata ctggatgggg aattaaaagt aattcattca gttaaggtgg caactacaaa
    11221 agatgttgaa actggtgttt acaatgcctt aaaaaaagtt atagatgaaa gtaagattga
    11281 ccattcatta ataaaatatg ctatgcttgg tacaactcat tgtacaaatg ctattgtaga
    11341 aagaaaaaga cttaataatg tagctatgat aagaatagga aagccggcca ctactgccat
    11401 tcttccattt atagattggc cagaagactt aagagaaaaa atagaattag attcaatatt
    11461 ggtatctggt ggatatgagt ttgatggtag aaagataaat gaactttgta aagatgaagt
    11521 tgtagagttt tgcaatagaa taaaaggcaa agtggaaagt gttgcaataa gtggagtatt
    11581 tgctccagta aataaacaac aagaagaaga agttgcaaaa tgggttagag aaattttagg
    11641 ggatattcct atatctttat caaatgaaat aggtaatata ggtatattag aaagagaaaa
    11701 tggagctatt ttaaattctg cattgtctca agtaggtaaa gctgtatctt taggatttga
    11761 aaaagcactt aaagacttag gtatagatgc tcaaatatat ttcagtcaaa atgatggaac
    11821 attaatgaat ttagagtata caatgaaata cccaattttt acaattggat gtggaataac
    11881 aaattctata agaggagctt ccttcttatc aaaagtaaaa aatgcaatag tattagatgt
    11941 tggtggaact acaagcgact tgggtgtact atataatgga ttccctagag aatcatctat
    12001 tccagttaca attggaggag tacatacaaa ctttagaatg ccagatgttt taactattgg
    12061 tcttgctggt ggtacatgtg taaaaggcga aaaagataat ataaagatag gtcctgaaag
    12121 tgtgggatat agaataactg aagatgcaat aatatttggt ggagacactc taactttatc
    12181 agatgttgtt acaaaattag atatggcgtt tgaagataga aaaagcaatg ttgaaaactt
    12241 agataaagag tattgtaatg aggtatataa gaaggttatt ttaaaattag aagatgctgt
    12301 agaccaaatg aagactagtc aagatgatgt tgaattggtt ttaactggtg gaggaagtat
    12361 aattgttcct gaaaaactaa aaggcataag caatgttata agacctcctc attatactgc
    12421 tgcaaatgct attggtgcag ctttaggaca aataagtgga gatgtagaaa aagtatacag
    12481 tttagataaa atgtcgagag aagatgccat agaagatgca aaaaaagagg ctgtaaataa
    12541 agctctaaaa gctggtgcta aagaagatac tatagaagta ttaaatatag aagatgttcc
    12601 acttgcatac cttcctggta atgcacttct tataaaagta aaagttgttg gtgatttaat
    12661 ttaaagaata aatataagta tttccagaat tatataaata tttctggact catttttaaa
    12721 cttattttat caataaaaac agcataactt ttgactatat tcttataagt ttccgaacag
    12781 ccgatatata cctatttttt gaggtttatc ggctgtttgt tatcttttat cgcattccaa
    12841 ctatttttct taccgtattt gctgtacgaa ttgtaatctt atcacttaca tttgaactgg
    12901 cagtctttga ccaccaattt gtctttttat aatctttacg actaaaagac caaaagatag
    12961 catttttata atgatatact ttttcatact cttctttagg atttccaacc tcatcataaa
    13021 actcgtcata agataagtca ggaaacataa aaataagatt atcatatatt tctttgttgt
    13081 tatctcccca ccaattagga gcattattta gtatatcttt taactcctct tgtgaaataa
    13141 taaaaactgg tatttccaat tcaaactgtt tttttatcat taatcttatt gtatttgaga
    13201 taaccttgtt gtcatctaca tcacttaaaa agacaacatt accactatta aggtatgtag
    13261 aaacttctga ataaccaagt tctacaaagc ttgcttttaa ttcagacata attatcttgt
    13321 tcttaccact tatattgata cctcttaata atgcgatata acttttcata taataagttc
    13381 acctctaaac attcacattt ttctatgtag tacaaactgg aatttgcatc ttttataatt
    13441 ctttcaaaac ttcttcatat gtaatttttt taatataaaa aaatcggtgc ttaatttgta
    13501 ccgattttta tttttcttag taaagaactc gtttttttgc ttctgctata gctctagcag
    13561 cactttttgc ttgcattctt ttttcttctt cttttttaaa ttcatttatt gtttcattta
    13621 gattcttatc atgttcattt ttttctttat cttcgttttc tctgtatact aatatataat
    13681 ctattattga ttcgtttatt atctgagata tacttttatt ttcattaatt cctattctct
    13741 taacttcctt aagtagagtc tcatcaatat gaaccgttgt ttgctttgta ttttttgcca
    13801 ccttaatcac ctcttaaaaa aaattctaac attaaaattt tttttagtca ataaatttta
    13861 atattaaaat tctttaaatg ttttatttga ttttctaaaa ttttctattg tatttatcat
    13921 cttatttttt atattttcac ctagtacaga agactttagt ctaggatttt ctatatggtc
    13981 ataaattggt tctaaaaatt cgacttcgac aggaacagag ttaattttac caataggttg
    14041 gtatccttca taagtatctt ttgaattttt tattacgagt ggaacaatag ggcattttgc
    14101 tttatatgct atctttaaag ctccacttct aaactccgaa accaatgaat ttggttcttt
    14161 aatccatgtt aaatctcctt ctggaaacac tgccatactt tgacctgtaa gtatattttg
    14221 agaggcttgg gctatactct ttataccctc acgattgttt tttctgttta catatacaca
    14281 tcttagtaat tttgcccatt ctttaaatat aggtatattt ctccaaactg gttcatctgc
    14341 aattacacat ccaattggtc tttctacact tgctgttagt atgaagctgt caagcatact
    14401 agaatgattt acaacaaata gaagtggttc ttttggcagt gtttccttac caattatatt
    14461 aagctctatg ttcactatat ctaaagattt tttagcttgt ttttgcataa attcaaattt
    14521 ttcctttaag ctaaatttat caggattctt tttaatttta ataaggctag gtatagaaga
    14581 tgtaaaacct ataagttgat atgtagcaaa ttttaagtaa ttattcataa aattatctcc
    14641 ttaaaataaa attatttatt a
//
LOCUS       DAEPLF010000075        14470 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEPLF010000075 DAEPLF010000000
VERSION     DAEPLF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14470
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.075"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     regulatory      complement(339..422)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(655..1497)
                     /locus_tag="KR314_001983"
     CDS             complement(655..1497)
                     /locus_tag="KR314_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0328366.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVMANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(1749..3533)
                     /gene="pepF"
                     /locus_tag="KR314_001984"
     CDS             complement(1749..3533)
                     /gene="pepF"
                     /locus_tag="KR314_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBH0328367.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEKYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(3599..4264)
                     /locus_tag="KR314_001985"
     CDS             complement(3599..4264)
                     /locus_tag="KR314_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBH0328368.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(4415..4576)
                     /locus_tag="KR314_001986"
     CDS             complement(4415..4576)
                     /locus_tag="KR314_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBH0328369.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(4654..5163)
                     /locus_tag="KR314_001987"
     CDS             complement(4654..5163)
                     /locus_tag="KR314_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBH0328370.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            5369..5587
                     /locus_tag="KR314_001988"
     CDS             5369..5587
                     /locus_tag="KR314_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0328371.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            5605..7551
                     /gene="feoB"
                     /locus_tag="KR314_001989"
     CDS             5605..7551
                     /gene="feoB"
                     /locus_tag="KR314_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBH0328372.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKSNTH"
     gene            complement(7711..8766)
                     /locus_tag="KR314_001990"
     CDS             complement(7711..8766)
                     /locus_tag="KR314_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0328373.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRANLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGTISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(8814..9614)
                     /locus_tag="KR314_001991"
     CDS             complement(8814..9614)
                     /locus_tag="KR314_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0328374.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     KVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFLIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(9607..10398)
                     /locus_tag="KR314_001992"
     CDS             complement(9607..10398)
                     /locus_tag="KR314_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0328375.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMFDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTTLINAIPKVILDGLGVAGTLLPAVGFALLMDMLISKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAMNTLTEGEIDFE"
     gene            complement(10438..10860)
                     /locus_tag="KR314_001993"
     CDS             complement(10438..10860)
                     /locus_tag="KR314_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBH0328376.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLANIEYFCMTKDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSIFL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENRNKIKNVM"
     gene            complement(10876..11355)
                     /locus_tag="KR314_001994"
     CDS             complement(10876..11355)
                     /locus_tag="KR314_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0328377.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTRDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKEIGDLGIDIELRMLPRDKKKQLSDLIK"
     gene            complement(11487..14243)
                     /locus_tag="KR314_001995"
     CDS             complement(11487..14243)
                     /locus_tag="KR314_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0328378.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSKFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNTILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEIFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALESKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINGPMLFIDQISTISALEVGNLLMQGKNIEQIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLPNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         4691 a         2493 c         1638 g         5648 t
ORIGIN      
        1 ttataaattt attatttttc tagaaacttg tctattggct agtttgagta ttgatttata
       61 tgatttatat ggttataata taaaataaat tgtattattt tggtaaatat attgttacaa
      121 aaggtttata ttttgcatct caatgtttat aaaaacttta ttttcttatt ataatagtat
      181 tatacttttt aaaaaatttc ccttttccaa caaatggcta ctttaaaagc caaaatttca
      241 cctatcaata aaaatcttaa cgactaatac tcttattttc aaattttgca acatttttgt
      301 taattttaaa gcaaaaaaag actatgcaat ataaaaatta tattcatagc cggttgcacc
      361 actaactcca tatatctcca gatgcccaat aataagatat acttctcagt ttctaaaata
      421 tttttattaa aaatctttta tatattttca taaatttctt ttttattcta acattgtaat
      481 attattttgt caatatttcc atatttttag atataagata ctaaaatacc tattttatct
      541 aaagatattt atatttctac attcaataat agacaatacc ctgcaagtta aagcatcatt
      601 tttatgagct tatctcacag ggtattatat gtttacttta tattataatt catgttattg
      661 attaacttta ataaatcctt tttctgaaac aagtgcttgt ccttcatcac ttaatatata
      721 atctattaaa gctttccctt tttctgacaa accatcttct ttattaacta atataaatgg
      781 tcttgatatt ggatatttat tagccattac atttgcatca gttgcttcaa catcatttat
      841 ttttaaacta tttactttct catcaacata tccaaatgat atatatccta tagcattttc
      901 atttccttca actgtagttt ttatatttcc tgaaccatca cttatttgtg catctttagt
      961 tagttcttct gaatcaaacc ctacaatttc ttggaatcca tctctagttc ctgaaccttc
     1021 ttctcttgat actattacta ttggtgcatc atttcctcct acttctttcc agttagtaat
     1081 ctttcctgta tatatacctt taacttggtc cattgtcagg ttttttactg agtttgcagt
     1141 attagttatc aatgctatac catctattgc tattttagtt tcctttaatc cagcactttt
     1201 ttcttcatct tttaaatctc ttgaactcat tcctatttca gatgttcctt ctatggcatt
     1261 ttttatacct ccggaagaac caatctgttg tacttctaca ctaacacctt gatttttttc
     1321 ttgaaacttt tctccgatag tttccataag aggacctatt gaagttgaac ctgaaacaga
     1381 aactttgctt gaatctcctg aatcacctga acctcctgag ttagaactac atcctactgc
     1441 taaagctcca actaacattg tactagctaa aagaacgcct attttctttt taaacatttt
     1501 acttaccccc gcttatattt taattttttg ttgtcatttt tctatatttt aataatacag
     1561 attctttgtt aaatgaatat tagagcaggg ttaagtaaat gtaaatattg ttaaatacat
     1621 gtaaaaactt aattttatat gatttattct taattagatt aactttttaa agttacttta
     1681 ggcagatata aacacaatac aactcatatt atcagaagtc ttcattgaag ctatcaacct
     1741 atcacatcct ataattcttt ttctagttta ttaactaatt cagcaaatac atttaaggct
     1801 tcctctatag attctttttt agtcatatct acaccagcag attttaattg ttctagtggg
     1861 tattctgacc ctccactttt taagaagttt atatatctat ctactgcagt acttccttct
     1921 gaaagtatct gttgacttaa agcacttgca gcagaaaacc ctgtggcata cttatataca
     1981 tagaagtttg aataaaaatg aggtattctc gcccattcta ttcctatttg tttatctact
     2041 attgcagatt ttccgtaata cttttcattt aatttgtagt atatattagt aaattcatca
     2101 gcagtaagtg gttctccact ctctaccctt tgatgtacaa ttttttcaaa ttcagcaaac
     2161 atagtttgtc tatgaactgt agttctaaat tgttctaaat aataatttaa taagtatata
     2221 gtttcatcct tagacttaga attttctaat aaataattta taagaagtaa ttcatttaat
     2281 gtagatgcaa cttctgcaac gaatatctta tagtccgaat ataaaaatgg ttgacttgtt
     2341 cttgaataat gactgtgaac agagtgccct aattcatgaa ttaaagtgaa tagagaatta
     2401 aggtcatttt tatagctcat taatatataa ggatgtgaat cataacttcc ccaagaataa
     2461 gctcctcctt ttttaccttc attatcatat acatcaatcc acccttcatc aaaagcactt
     2521 tttatgacct tcaagtattt ttctcctaaa ggtttaagtg cctctaatat gatatcctgt
     2581 gctttatcat atggtatatc catgtcaaat ttatctgtca aaggaacata caaatcatac
     2641 atgtgtattt cattcaatcc taaaaatttc tttttaagct caacatattt atttagatta
     2701 ggaatacttt tatctatagc tgatataaga ttattgtaaa catctaaact tacatcatct
     2761 gaaaataaag acgcttctat tgctgaatta tactttctag tttttgcgta aaatatctct
     2821 gattttattc caccataaag cgttgaggca aatgtatttt tatatttctc atatgttgaa
     2881 aattcacctt caaaagcgtc ttttctaact ctactattct tactttttat aaataaagag
     2941 tagtttgaat gagaaagctt aaccttatta ccctcctcat cttctatctc aggaaattcc
     3001 atatctgcat atgctaacat atcgtataca ttttctggaa ttgtcgataa ttctgatgta
     3061 gctgcaagta tttcttcctc tcgttcactc aaagtatgtg gtttatctct caatatatcc
     3121 tttatatatt ttgtataaaa ggatagcttc tcatctttta agtattcact taacttattc
     3181 tcttctatcg ccagtatttc tggaactata taagaagtag ccacacctaa atcagtagat
     3241 aacatttctg tcttagtggc tcttccttga ttatcattaa ttctagtatc ttcatgttgt
     3301 ttcatatgag tatatacata taaattttga acaatcctag atgcattttc agagatattt
     3361 aatactttat ataagttctc cttactctca gaaagcttcc ctttatattc ttttacttct
     3421 tggataagat tttttacttt acttatgtct ttttcaattt cctctttact ggagtacatc
     3481 ttatctatct tccatttgta cttttcttct attgtacttc tatcagttcc catatcatac
     3541 acctctattt taatttgttt tatattttat tcactttaca aaatatattg tatatatttt
     3601 agtccaaata atctacattt attactcctt ctatcatatt tatttctttc ataacgtcgt
     3661 gtaagtgtat tctcttagca taagatacaa aaaaattaac cttccagtat tcttcattac
     3721 gacattctat ttccatattt cttacgataa gttgattttt ttcaaattta tcatataaaa
     3781 aaggtataga gtccaaattg cttaatttta atatcaatac attcttttct tggctaaata
     3841 aaaacgtatt gattatccta agcacaaata atgctatcat tacaaataat ccacctagaa
     3901 ttgccaattc ataataacca tatccaactg ctataccaat acatgcagtt gcccacatcc
     3961 cagcagctgt agtaagccct tttactccac ctttattttt taaaattgct cctgcaccta
     4021 aaaaaccaat tccagataat acttgagcag gcattcttgc tgggtcagca tttgtataca
     4081 aattataatc aaagaataat tgaatcgaag ttattgaaac aatacaagaa cctattgcaa
     4141 ttagtatatg tgtcctaaat cctgcaaact gtcttatttt ttctctctct aagcctatta
     4201 ttcctcctat tattagtgct aatagtaacc ttattgctat ctccttactt cctatatcaa
     4261 tcataatata aaaccctcct gacttaatta caacttatgt cctacagata atttacacat
     4321 cagaaatact attttatatc ttaatattat tattaatata tgtaaaatat agccatctgt
     4381 taatttttga tacaaaaaaa cccacaactg tgggctattt gcttaggtac atgtatttat
     4441 ttattaatat atctattttc ttacttgatt ctactaactc ttcttgaaat atttctggat
     4501 attctatgta tgtatttatc tcttccctaa ctgcttcaat ctcatttttt aacgcttcta
     4561 attcattgtt cgccataata atcaccacta ttacaaattt gtatattttt atatacattt
     4621 tatcaaacaa tattatattt tgtcaatatt atgtcaataa aacttactat aaaaatgctt
     4681 taatttaggt attgtatacc tggatactaa gcctacaaac ataccagtta ctaatgatgc
     4741 cattaacata aatggtaagt accctatagc aagtatattt tctatgatta aacttgcaac
     4801 tattaattga cctatattat gccctattgc acctataatg cttatcccta ttagtgataa
     4861 atcttttatt ttactcgcta aatacataaa taaaagactc agataagctc ctcctatact
     4921 aaatagtaga tatgataaag ggcctgcaaa tattgatgac aaaattaccc tgatagtaac
     4981 aattataaca gtatctttga cacctaacaa cattaatgaa attaaagtta taatattgct
     5041 caaacccaat tttgcccctg gaaatataac tataagagga tttggtatat atgtctctag
     5101 tacatataaa gctaaactat accctatcat aagacctaaa aatgtcattt ttctagtttt
     5161 cattaagtta actcctccaa attttatcta tcatcttaca ttataataca aaatatataa
     5221 aaacacctaa tttagactaa aatattttta atctatttag aataaatatt agttatttca
     5281 atgtgcaatt acctctataa atcttgactt tttgtatcat acttgttaca attttaatga
     5341 taatgaattt cattaggagg tgttaataat gactgtttac gatttaaaaa taggagaaaa
     5401 aggatttatt aatagtatag atggagatat taaattatct aaaagattgc ttgcacttgg
     5461 atgtataaat agtactgaaa tagaagttaa aaaggttgcc ccacttggag acccaattat
     5521 aattagattt agaggctttg accttgcgat tagaaaatct gatgctaaaa atatttcttt
     5581 acaataatag aggaggagct acccgtgatt aatgtagcat tattaggaaa cccaaatgtc
     5641 ggtaaaacta ctgtttttaa tctacttaca ggttcaaatc aatatgtagg taactggcct
     5701 ggtgttacta tagaaaaaaa agaaggattt cttggaaaag agataaaggt tgtcgattta
     5761 cctggtatat atgcaatgga tactttctca aatgaagaaa aggtttctaa atcatattta
     5821 gaaaatgaag atgttgatgt aatagtaaat gtagttgatg cttctaattt atcaagaaac
     5881 ctttacttaa caactcaact tatgcaattt aacaagccta tagttatatt gcttaatatg
     5941 ttagacattg cagaatctaa aggagttaat atagatgcca aaaaattaag tgaagaactt
     6001 ggagtaattg ttgttccaat tatagcaaaa aagaaaaatg gtgttgatag gatagaagaa
     6061 gagattaagg gagctacgca aaaagctttt atatatcaga aaaactttgg aagtgaaaca
     6121 gaaacatata aaaaattaga atccatttta tcgagatgta caaaaactac atatacacaa
     6181 aagaaatcta tcagtgataa gatagacaat atagtcttga atcctatatt agcatatccg
     6241 atatttattg gtgtattgtt cctcttattt aaatttacct ttgactgggt cggaggacca
     6301 ctacaagagg gatttgctag tcttatagag gcttatattt ctgcacctgt tagtgatatg
     6361 ttatctaact ctagcccatg gtttaaatca cttatagttg atggaataat tggaggtgtt
     6421 ggtggtactt taccattctt ccctttaata tttacattat tctttggtat atcgttgttt
     6481 gaagatagtg gatacatgtc aagaacagca tttttaatgg ataaagttat gagaaaggtt
     6541 ggtctatctg gtaaagcttt tatacctata gttatgggta tgggatgttc ttctccagct
     6601 ataatggcaa ctagaacatt ggaaagcgaa aaagatagaa gagttacagc tcttattgca
     6661 ccacttatga cttgtggagc aaagctacct atatatgcac tatttgtagc tatattcttt
     6721 ccacataatg ctgctttagt aactacgtca ctgtatttgg tgggtatagt tatggcaatt
     6781 gtagttgcat tattcttaaa taaaacagcc tttaaaacag aggctgaacc atttatatta
     6841 gaattaccag aatatagaat ccctactata gatgcactta ttaaaaatac ttggaataaa
     6901 tcaaaaggtt tcttgataag agttgttact gtcatgtttg caatgtcagt cgtaatttgg
     6961 ggactttctt cattcaatgt ttttggatat acagaagata taaatgtaag cttcttggct
     7021 actctaggtc atattatatc tcctatattc aaacctcttg gttttgatga ttggagaact
     7081 tcagttgcta ttttaagtgg tcttggagca aaagaaatcg tagtaaactc tttaaatata
     7141 ctatatggta atctaactgt tgtcttacca acaatattta atggagttac tgcatataca
     7201 ttcttgatat ttactgcttt atatactcca tgtatagctg cacttgcaac tatgaaaaaa
     7261 gaatatggca ataaaatgat gtttacatca tttgcatatc aatttatact agcatggata
     7321 atggctttta tagttaaaag cataggtgga gtactattta tgggtaattc aataatggaa
     7381 gctttgatag gtggaataat tatagtcctt gcactcttta ttctgtttaa taaattcaag
     7441 tctacaaaaa acggaaatgt ctgtagtgga tgttctattt gctctggatg tccaagtgct
     7501 aattcttgct caagtaagga agacagcaat aaaaaaagta atacacacta atatataaat
     7561 attttagttt aaaataagat aaaagtgggt gtctcaaaat taacttttta gttcatgagg
     7621 cactcttttt tatataaaat caaatatatt ttcatcattt caacagtcaa aaagaggctt
     7681 atctctattt tgagacaacc tctttttttg ttacttattc agtttcttgc tacctaactt
     7741 catgtggaac ctagtatctt ttggaacact tgggtctacg cctcttaatg taggtatttc
     7801 cccacatatt agttgtatag gtactataaa attaaatgtt tgatagtaag gattatctgt
     7861 gatatcaatc tttaaatttt tatcattttc tgtaacatct ctacctatag caaatacatt
     7921 ttccgtccaa ccagaaagta catcaatcat cttagttact ctaggttctt ttcctgtatc
     7981 tagtataaat atagtagaat cactatttat agcattatat ataccgtgta taaactcttc
     8041 aaactcatac ccagttacag gtattctcat tgtttccaat aattttaatg ctgcttctaa
     8101 tgtatctcca tatatatcag agtgacctat tattcttatt tctttagaat taactaactt
     8161 ttctttgttt ctttcaatcc attgtttaga gagcttatac actgcttcaa atctattaat
     8221 tgcatctaat atcttattta tttcctcatt gtacttttca ctagatattg tacctttttc
     8281 tctagctatt tgaagaccta ataacattaa gtttaacttt gtacaataat atccttttgt
     8341 tttagctcca gatttttctt caccacaatt tactgttaat atataatcag aaatctcatc
     8401 tattaaagca ttcttacaac ctgccataga tgctatttta caacccttat cttcagctaa
     8461 cttcatagcg ttgtatgtag aataactact cccaccttga gatacaccta ctactagggt
     8521 attttcatta tcaaacttga atgtatcttc agttatcata aatggataca ttttcacaac
     8581 atctatatct aataaatttt gtaaatatgg ttgcacttgc actcctgagt gatagctagt
     8641 acctgaacct gttattataa tcttttttag atttgctctc ttaacttcat taaataagct
     8701 atctgcatta gatataattt ctctcattct aactggtgtt tctaacatat aatcttgtat
     8761 tgtcatttta tacaccttcc ttatctatat attcaataaa ttataattaa tttctataac
     8821 aaacctatag catgtaaagc gattcctgtt actatagtgc ctatagttaa ttttgttaca
     8881 cttacctttc tcttcaacat gtaatatatt aaaaatgcaa aggttaaagg taacatatta
     8941 ggtattattt tatctagtat atcttgtaaa attacttctg caccatttaa gttaaatttc
     9001 aaaggagtag ttatactcaa catagttgca accattcctc ctacaacgaa taaaccgaca
     9061 gttgtagcca tagacattat cttctccata agtccctctc tttgtattct ttcaagagaa
     9121 tttaccccta ctttataacc atacttaagt ccaaatattc taagggcaaa tgctggtata
     9181 ttgtataata ataagaataa tagtggtcca aagatattgc cttcttttgc aagtgatact
     9241 cctactcctg ctgctataat cctaaatgtt ccccagaata atgaatcacc aattcctgca
     9301 agaggtccca taagagctgt tttaacagca ctaattgaac tttcatcaaa ttctggatta
     9361 ttagcatttt cttcttccat agctgctgtt atacctaata tagcaggaac taccactgtc
     9421 gttgtattaa aaatctctaa atgcctttct attgcttttg cttgattttc tttcttatta
     9481 tatagcttct ttattgcagg taacattgaa taacaataac ctaagttttg cattctttcg
     9541 taattaaatg caccttgaag agcaaatgaa cgccaaaaca tacttcttaa ttcttttttt
     9601 gttaatttat tcaaagtcaa tctctccttc cgttaaagta ttcattgcta attgaggatt
     9661 ttgattattt tctttgttat tgatataaaa gtttagtaca aatgctacac atactccaaa
     9721 taaagctata gcagttatat ctaatccacc ataagatgct aacaaaaacc ctataaagaa
     9781 aaatacagac atcttttttg aaatcaacat atccataagc aacgcaaaac ctactgctgg
     9841 caacaatgtt cctgcaacac ccaatccgtc taatataact ttaggtattg catttatcaa
     9901 tgttgtaact ttatcagcac caatcataat tgctaaaaag gttggaactg atgtacttaa
     9961 aaagaataat attactggca accacatcat tatggtaact ttcttaaaat cagcctgttt
    10021 agcatagaaa actgcctttt gagaaaagta cgaatttatt attctaacta atactccaag
    10081 agattgtgct aatactgcta ctggcactgc cactgctaaa gcaacttctg caccattacc
    10141 tgataatata gcaaaagctg tacccaatac acctcctgtt actacgtctg gaggagtagc
    10201 tgcacctatc cctgcaattc ccatccaaat taattcaagc tgtgcaccaa tcataattcc
    10261 tgattttatg tctcctaaaa ctaatccaac taacatacct gtaactattg gcctatcaaa
    10321 catagattga ccaaatatac gcccatctag tataccaact cctgctataa tagctaacat
    10381 agtagcttgt actaacatat tgaacctcca ttttaactta gtttaattta cattaaatta
    10441 cattacattt ttgattttat ttcgattttc taataactca tttacataaa caattccatt
    10501 ttttgcttcc tctaagcctg atttaactat tagttttgta tcctcctcaa ctaataagaa
    10561 tatagataaa agcattggta aatttacgcc tgtcaacaat tcaaatttga acccttgcaa
    10621 aagtaagttt gtacatatat ttgccacact tccaccaaac acatctgtta aaacaacaaa
    10681 ttcttcacta tctttattat ttgataatat acttctaatt tctttctcag catcatctgc
    10741 tgatttatct tttgtcatac aaaaatactc aatattagct aatgaaccaa ttattaattt
    10801 acttgtgtca acaagttctc tagacaattc tccatgtgtt gcaattagaa attttttcat
    10861 tctctttctc ctaaattatt ttattaaatc tgatagttgt ttctttttgt ctctaggtaa
    10921 cattctaagt tctatatcaa ttcctaaatc acctatttct ttaaagttat ttatatcatc
    10981 atcatcaaca gcaactaaat ctgtaagttt cttttttcct tcttcatatc ttaaccctcc
    11041 tacatttatt tcttttaata ctcctcctga atttttagct acaactaaag catctctggt
    11101 attttttact aacactaata cattgctttt ctttgaagaa gcaaattttt ctactatttc
    11161 tccagattcc tctacccccc ttatatataa agttacattg ctaggtttcg ctaaattcag
    11221 tgacatagct ttcatttggt cattttttgc ttcatcattt gcaactagta ttacatttat
    11281 acctagtgat gttgcccatc catatgcaac ctgaccatgt atcaatctgt catcaattct
    11341 catcaaagat accattttcc ccaaatcctc catcatacat taaaattttt attgtttata
    11401 ctctcattta tctataaaag caaaaagtat gccaaatact ggcatactat tttaaatttc
    11461 atttacttat tttgtaatct tgtattttac tgtggtagac catagataat attataaata
    11521 tatcctatct ctgaaattgg tatttgcaca ctataactct tttctagcac actaaatgca
    11581 ttttttatta gttttacttc actatgatgg tttttgatta acaaatccat atcagtatac
    11641 tcttcaattt cagtttgtct aactaatctt tcaatcatac aacttaaatg aacatataaa
    11701 gaaatctttt tatcatttgg aagttttttc ttttgaatat tttctatagc attgattcct
    11761 tcttctattc tctctattat cttttttgaa tctaaaatag ttagactgtc tattactcta
    11821 attaatgaaa aattctttac aatagaatca tttatgttct ttattttttc attttctact
    11881 actctactaa atattctaaa aacatcatct tctccttttc ctgatattat atcctctaac
    11941 aatataaaat ccaccccatt cacatttgga ttagcagttc ctactatagc catcacatca
    12001 tacatttcaa aaactggttc tttatctccc atagaaacaa gactatcata atcataaggt
    12061 attattttga catcaactat cccaattaaa ctttcggaaa gtagatgttg gatttttata
    12121 gcagtaccta tccctgtaaa acaagatgat attatagcat attctttatt tttttgagga
    12181 taatacaatt gtatattagg tttagtttct tcatccataa tttctgcaat ctgttcaatg
    12241 tttttacctt gcattaataa atttcctact tctagtgcag aaatagtgga tatttggtct
    12301 ataaataaca ttggcccatt tatcttattt tttaaatctt catagataag attaagagag
    12361 cccatatcaa ataacacaat taaaccattc tttatcctat ctccttctag gtatctagac
    12421 atttgttttg atacatcagc aatggtagca tctataggca tatcaatagc tgtaaatata
    12481 tttttaccca acatccgatt acatacattc gccatactac tggcagtagc atatccatgt
    12541 gctataatag tcgcattaac ttgattaaat ttcattctta taattgttga tttaataaat
    12601 ataatcatta tgattgtatc tacattatta agatacatat ccaatctatt cgttattaat
    12661 gttgatattt tacttattat agagtattct ttgtaactct ctttttttaa atatattagt
    12721 aatttacttt ctaaagcttt atactcatca gataattgat aagattcgtc cttcaaataa
    12781 agataagacg aaaatgccaa tacattattc ccatcgtatt ttatattgtt attaacttct
    12841 aaatttctaa atatatcctg cattatcgat attataagct ccataagtac gttattattt
    12901 tcatcattat ttgaaaagac taaattgtcc attagtctaa tcattagatt agaactcttt
    12961 ttactaaata tttcttttgt tatttcattt ttttctagtc tatcaaagta gtctaaacat
    13021 tttatgaaac tttgatagat tatatttctt ttatttcttc catttgacat ttcaatacta
    13081 aattgtttag gagaaaagat aatgcttttg cttttaccac tttggaattg aaatatatct
    13141 acattttcta tataaagatt tctagggagg tcttgaagag taacaaaaat atttccattt
    13201 cttctttgat tttttgcata agctcgtcca catgcatatt ttattacatt tttacattca
    13261 cctatattcc ccttgaattt tatctttaaa atcgtattta atgtaatttg agttatctct
    13321 ataggcaagc caagtacctt actttcttca taaataaaat gttctacaat ctttcttttt
    13381 tcaatagtcc ctctttcttc tatattggga acataaatat ttatgggtat tcttcttaaa
    13441 aaagtttgca aaaatttgga tagactttct gttgttgcaa atattaatct taccctagct
    13501 tttctaatta ctccattctc tccaatccta gaaaacgtac ctgtatccat aaatgtaaat
    13561 aacttttcct gaccttcact atttaatcta tgtacttcat ctaaaaataa aacaccacca
    13621 tctgattgtt ctattagacc tgctctagat tcatttgctc cagtatatgc tccttttaca
    13681 tgcccaaata atagactaga caataactcc aaattattat aatactgcgc acagtttaac
    13741 acattgtatg gagaattttt ttctaataca ttactgctta cactatattt gtatattatc
    13801 ttggctaaaa aagtttttcc tactccactt tcacctgtca gcattattgt aagtccgcca
    13861 tttggataaa gtacagcggt ttttatttgt tcgattactt cttttaagct attatcatat
    13921 cctatgatac tattaaacac agtctcttgc tttaataatt tacactcatt tttaagttca
    13981 tctaaatttt cataaatact tttttttgta ggtcctacta catcatttat tttattttta
    14041 ggtaaaaaaa taacaggtct actgtttatc tttataagat tatcagattt tactaaatca
    14101 tttaataatc tacttgattg agttctactt aaattaaact tttttactat atcttctgtt
    14161 attatgccac tttctttttt aagttcatca aagttgttaa ataatacaca ttcatttaca
    14221 aaacttaaaa tttcatccct catgttcaac ctccaataca aatatcaata atactataat
    14281 attagtatat catttatata tttatgaaaa agataattat attcctatct tgattctctt
    14341 ttgtaaggtt gtaaaatcaa actttttata agtataaaat ctagattttt taacataatc
    14401 tcaaggttct tatctttcca tatatctgac atagaattta tataagaata tatttattgt
    14461 atttattata
//
LOCUS       DAEPLF010000076        14443 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEPLF010000076 DAEPLF010000000
VERSION     DAEPLF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14443
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.076"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            56..3154
                     /locus_tag="KR314_001996"
     CDS             56..3154
                     /locus_tag="KR314_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH0328379.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGINDISHVK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKFKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFIVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMEVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFDDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDILTGLYN
                     YESFCEQTSLMLKRYPDKSYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDVHKLIYKIKNSLNRKKFEYMLVPCFGVYKILDNN
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLRHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            3277..4563
                     /locus_tag="KR314_001997"
     CDS             3277..4563
                     /locus_tag="KR314_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0328380.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAISENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(5149..6255)
                     /locus_tag="KR314_001998"
     CDS             complement(5149..6255)
                     /locus_tag="KR314_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBH0328381.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVTTEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DPLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(6290..7831)
                     /locus_tag="KR314_001999"
     CDS             complement(6290..7831)
                     /locus_tag="KR314_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBH0328382.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            complement(8193..8939)
                     /locus_tag="KR314_002000"
     CDS             complement(8193..8939)
                     /locus_tag="KR314_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0328383.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCNSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            complement(9151..10461)
                     /locus_tag="KR314_002001"
     CDS             complement(9151..10461)
                     /locus_tag="KR314_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0328384.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            complement(10474..10944)
                     /locus_tag="KR314_002002"
     CDS             complement(10474..10944)
                     /locus_tag="KR314_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328385.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(10948..11799)
                     /locus_tag="KR314_002003"
     CDS             complement(10948..11799)
                     /locus_tag="KR314_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBH0328386.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            12724..13635
                     /locus_tag="KR314_002004"
     CDS             12724..13635
                     /locus_tag="KR314_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBH0328387.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            13810..14307
                     /locus_tag="KR314_002005"
     CDS             13810..14307
                     /locus_tag="KR314_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBH0328388.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNAIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVILAGKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
BASE COUNT         5486 a         1823 c         2243 g         4891 t
ORIGIN      
        1 ttttaaaagc tttaaaaatt caatctggaa aatgaataaa aggagatata tataaatgga
       61 tgtaataaat ttaatagatg agttggatga aattgtatat gtttcttctt tagatacata
      121 tgaactttta tatattaatg aaattgggaa aaaggcaatg ggaattaatg atatatccca
      181 tgtaaaatgt tataaagcat tacaggggaa agattctccc tgcgaatttt gtacaaactc
      241 tattttaaaa gaagatgaat attacatatg ggaaaatacg aatactcgaa caaatagaca
      301 ttttatatta aaggacaagt taattaaatg ggagggcaaa catgcacgat tagaaatagc
      361 tcttgatatt acagaaaagg aagatataag taagtcaata gcagaaaaat tagagataaa
      421 ggagttatta gttggatgta ttaaacatct tataacagaa tctaattttt ctgatgctgt
      481 aaatcttgta ttaaccaata ttggtaaatt tcataaagct gatagagcat atgtttttga
      541 agtatcagaa gatagaaaaa aagtaactaa tacatatgaa tggtgcaatg aaggtgttga
      601 tgaacaaaaa caggcactaa caaatcttga tattaatgaa ttaaagaatt ggtttttatt
      661 attcgaacaa aagggttttg taataattga tgatatagaa aatataaaag aagaacagcc
      721 aatagaatat gaaattttaa aacctcaaaa aattgaaagt ttaatgacat cttcactaaa
      781 acaagatgat aaagtagtag ggtttattgg agttgataat ccacataagg ctgttggaga
      841 tacctctctt ttgacatccc tatcttattt tttattaact gagatgaaaa aaagaaaagt
      901 agaaaataaa ttaaattata tgagctacta tgacttcttg actggtgcat ataatagaaa
      961 caaatatatt aagtatatag ataattttca aaaaggaaaa tttaaatcca taggagttgt
     1021 atttgtagat ataaatgggt taaaagatat taatgataac tttggacatg ctaatggcga
     1081 ctctgctatt gtgtcagcat gtgtaataat aaaaaaatac tttcattctt ataatgttta
     1141 ccgtattgga ggagatgagt ttattgtatt atgtgaagat atatcaaaac aactatttga
     1201 agaattagtg agttggttaa gtgaagaatt taaggatgag atgatatata gtgtagcagt
     1261 gggcagtatt tggagtgacg ataatattga tatacataag ctaattaaag ttgctgatga
     1321 gagaatgtac caagataaga agaggtatta taataagaac aaacttagaa ataatgaaat
     1381 ggaagtaatt agtgacttag ctgattcgac gaataatatt ttatttaaac ataaagagtt
     1441 agttgataag gtttattttg atgatttaat gataaaaggg tattctaagg cttatgaaaa
     1501 attagcttat aatacatatg atataatttt atctatttgc atcgatacta atttaattac
     1561 tccaatttat caatcagaga gcaatatttt tagttttcct ataaaggaaa atcttgaaaa
     1621 aactttttca tacataaaaa aacatttaat tcatcctgat gatataatat tatttgatga
     1681 taaccacctt ctaaaatctc ttgatagtct taataacagc aagcaagacg atttagaact
     1741 aaattttgag tatagacgtt taggaataga taaaaagtac tattgggcat taatttatat
     1801 gttaaagatt agtagtaaaa ataagttagg tgagaaacaa acacatattt tagttgctat
     1861 aaaaaatata gattatctta tgcaactaca taaaaaccaa aaggatatat taacaggact
     1921 atacaattat gaaagttttt gtgaacaaac aagtttaatg cttaaaagat atcctgacaa
     1981 gagctatgca attataatga tggatataga taagttcaag gtaattaatg acatttatgg
     2041 agttagagga ggagataagg ctttaatata tgttgcagaa ttgattcaaa aaaacattgg
     2101 ttctaatggt atatgttgtc gtatgtatgc agatgtattt tgtatttttt atgagagtta
     2161 ctctgataga gatgttcata aattaattta taagattaaa aatagcttaa atagaaaaaa
     2221 atttgaatat atgttagtac catgttttgg ggtttataaa attttggata ataatattcc
     2281 aattacaaat gcgtgtgaaa tggcaggata tgctcacaaa aaagtaaaga aacaaagtat
     2341 caataactat atattttatg atgatactat aagaaatgat gcacttgaag aaaaacagat
     2401 ggaaagggaa atggaatacg cacttgaaaa tgggcaattc catgtatatc tacaacctaa
     2461 gtataatctt aatgcaaata aaattgttgg tgcggaagca ttggttagat gggttcatcc
     2521 agaaaaaggt ataatacctc caattaaatt tatttcacta tttgagaaga atgggtttat
     2581 tatcagatta gacatgtatg tgtgggaaca agtatgttct ttgattagaa aatggataga
     2641 tttaggtgaa gagccagttc ctatctctgt aaatgtatca agattacatt tgcataatcc
     2701 acattttaaa gaaattgttt taagtttaat aaataaatat agtataccaa aatctttttt
     2761 agaattggag cttacagaaa gtttatttat taaaaatata aaattattta ctaagataat
     2821 agccgacctt agaatggatg gtattgtttt aaatatggat gactttggtt cagcatattc
     2881 ttcattaaat atgttaaaaa atattaatat tgatacattg aagatagatt gtggcttctt
     2941 taatgaggaa ggaatcacag acagagaaaa aatagtttta aggcacacta tagccatggc
     3001 aaaagattta gatatggatg tagtggcaga aggtgtagag acaaaagaac aagctgaatt
     3061 tttagtgagt ttagattgtg tggtaattca aggttattat tattgtaagc ctattccaag
     3121 tgctgatttt gagaagttac tttatcaaaa ataagctaat tatcccactc taaatgtagg
     3181 taggatggag tagttcaaca taattaaata gaagtagaaa atagtgagga tataattttt
     3241 aaaaagtata atatttatgg attaggagcg aagtttatga tagtgggtat tataggtcct
     3301 tcagactctg ggtttaagat aaaggatgat ttaaaacaga tagacagtga tttaaagact
     3361 aaaatatatg taagagaaaa agtagtagat acaatagaag ttatttctaa atgtgaagat
     3421 gaatgtgatg ctattatatt tacaggatgt gcagtttatg aatttataaa atctaagtat
     3481 gagataagta agccacattc atttgtaccg agaagtggga cgagtataat gaaggcattt
     3541 tgggcaataa aaagtgcaaa tataaaatta gataaattca gtatagatgt agttgataga
     3601 tatgttgtgg aagatgcact taaagaattt gatattaaag caacgagtgt attttgtaat
     3661 ccattttctt tggaagtagg agagtctgaa cttgtagaat ggcatataaa attatttgaa
     3721 gaaaataaga cggatataat gttaactggt tttggtgcag tatataatga attaaaagaa
     3781 agaggctatc ctgtatttcg tttacaagct actatacaat taatcaaaga aagttatgat
     3841 aaggtaaaga gtgaatatgc tctaagcaag gcaagatttt ctcaaattgc tgtcgaaata
     3901 ttgagtctaa tagattataa agagaagata gataactatt attctgacat gataaaaaaa
     3961 tcagatatgg ataagcttgt agtaaactat gtaagaagca ttcaaggttc cttattttta
     4021 cttggaagaa atgattatgt tgtatttgtt cataaagggg tagctgataa tgagtataat
     4081 tatgataagc tattcaatct aaagagagag ataaaagaca tgggcttttc tcttagtata
     4141 ggaattggta ctggagtaac tgcgtatcag gctgaaaata atgcacataa agcattaaga
     4201 cactccatag attcaaaaga aattggtata tatttggtag atgaagatga aaatataaga
     4261 ggacctttag cttccgaaaa tgaattaaat tattctttga tgctatctga tgaaaaggta
     4321 atagaaatat ctaaaaagac tggaatgagt tgtgagtcag ttgcaaaaat tattgctata
     4381 agtgagaata gaaaaagtaa gatttatgat tcaaaagagt tagcagagta tctaaatgta
     4441 agtgaaagaa gtgcaagacg tatattaaat aaaatagtaa atgcaggttt aggtcgaata
     4501 tgtgcaaaag agactagtat tggtggagga agaccaaaaa atattactga aatactgttt
     4561 taagatattt tataaaactg tataaatcat tatattttat aaaattctac aacaaattac
     4621 tatataaagt attaaagctt atcatgaaaa aaattagaac aaattattat ataaaatact
     4681 agagcttgtt attaaaaata ctagaataaa ttattaatat aaaatactag agcttgttat
     4741 taaaaatgct agaacaaatt attataaaaa tactaaagct tgttattaaa aatgctagaa
     4801 caaattatta tataaaatac tagagcttgt tattaaaaat actagaacaa attattatgt
     4861 aaaatactaa agcttgttat taaaaatact agaacaaatt attataaaaa tactagaact
     4921 tgttattaaa aatgctagaa caaattatta tgaaaaatat tgaagcttat tatgaaaaat
     4981 gttagaacaa attactatat aaaataccaa agcttattat gaaaaatgtt aaaataaatt
     5041 attatataag atactaaaac ctatgtaaaa caactttaat atataactta taaagttgaa
     5101 gatacatagg tttttatttt tataatttaa agaggtatat tatgatattt ataatttaat
     5161 catatgataa actattttgg cagtatcata taattcgtct atatttacat attcattaac
     5221 tgtgtgtaca tcatacatac ctagacttaa tatagcacaa ttatatccaa gattagctaa
     5281 tatatttgcg tcacttccac caccaattac cattggattt ggaacaacac caacttgtct
     5341 tattgcttct tcacttaact tgaaaacgtg actatctcta ctaagttcaa aactagggta
     5401 agacatacta tgttcaaagg tataagtagt attaaattta gatgcagcat ccttacaaca
     5461 tttttccata tgattaagct catactctaa agtttcaggt atatgagacc taatttcagc
     5521 agtcaatgta accttatcag ttactatgtt tgtagctcca ccaccttcta ttttacctat
     5581 atttgaagta gtcaatggat ctattctgcc tatgtgcata ttgcttattg catgagaggc
     5641 tactaatata gcatttaacc ctttttcagg ttctatacca gcatgtgctt tctttccatg
     5701 aaaagagatt tttatagatt gttgagcagg agctttgtat gctatcgaac caatagctcc
     5761 accagaatct aaaatcacca tatctttaca aggcaagttg tcaggattaa agtttttgac
     5821 accatgcata ccagcttcct cacagattgt aaaacaaaga taaatatctc tatgtggtat
     5881 tttttctgta gtaacatgtt ctaaagcttc aagtatagca gctattccag ctttatcatc
     5941 tgcaccaagt gtagtagttc catcacttct aactacatta ccatctagta ttggttttac
     6001 acctaaacaa ggttgaactt ggtccatgtg tgcacataaa caaagtggtc tacttccttc
     6061 ttcacctttt atataggcaa tgacattacc tgtattacca ccatattttt cacctgcatc
     6121 atctataatg gcatctatat ttctttcttt taagtagttt accaaccact tagacatttc
     6181 taattcttga ttagatgggc tatcaattct aaccatatcc ataaaattat ttataagtct
     6241 atttttatct aacataaatt atttctcctt ttagaaagat ttaaataaat tacatgctat
     6301 atgaaggaag tattccaggt cctagtggaa tattaaatac cataaacaat attaatagta
     6361 tagtccacac tattaagaat gatatagagt atggtatcat attagcaatt acagtaccca
     6421 taccagcatc tttatcgtat tttcttgtga atgctagtat aatagggaag tacggaaata
     6481 atgggctaag tggatttgtt atacaatcac ctatacgata agccatctga gtaagtgctg
     6541 gattgtatcc aaccatcata aacattggta caaatatagg tgcaagaata gcccactttg
     6601 cagatgcact acctataaat aaatttataa aacatgataa tataataaat cctatcaaca
     6661 ataaaggtcc atttattcct gctgatttta aaaattctcc gccctttaca gctagtatca
     6721 gacctaaatt actatttgtg aataattgca aaaattgtga agcagcaaat gctagtatta
     6781 tatatccacc catatcactc attgcactag ccattataga gactacatcc ttatccttct
     6841 ttattttacc tatagttttt ccgtatacaa caccaggtat gaaaaatgct agtgctatta
     6901 taggtaccat acctttcata agaggagcat tgtttgatag taaatctcct gtttctgggt
     6961 cagctaagaa tggtttttta ccgattacac taagagctac tataatagct acatatatta
     7021 atatacttat accagctttt ttaagccctt tcttttctac atcactaaga cttccatcaa
     7081 catctagttc tgcatttcct tcatatttac caaatctagg agctacaatt ttttctgtta
     7141 cccatgttgc caaaagtatt agaacaaaag ttgatgctat cataaaaaat aagttcattg
     7201 ttgcattggc ttgaaatcca gaatcaatca tttgagctgc tggaactgta aaacttgcag
     7261 caagtatatc attgacactt atcataatgt ttgcggcaaa tcctagacag actgctgcat
     7321 atccagaaaa taaacctata agtggatgtc taccaacacc aagatataca agtgcagcta
     7381 atggtggaaa taatattgta cctgcatctg aagctatatt ggcaagcata cctatagtca
     7441 ttacaacaag agttactctt gtttcaggaa tcttgcttac tgatgctttg attgttgctg
     7501 tcataagtcc tgttttgtca catacaccag cacctatcat agttactaga actagaccca
     7561 aaggagcaaa cccttggaaa ttgctaacta tgtttcctaa aagaatttga agctgttcta
     7621 cagtcaataa atttactgca gttatagttt ctccagtacc aggatgaact actgatatcc
     7681 ccatagaacc aactagacta gatagtataa gtaaaagtac acataaccct aaaaatattg
     7741 taactgggtc tggtagttta ttaccaacag tttctattct gtttaatgtc ctgtcaaaaa
     7801 agtttttctt cttaactacg ttatcattca taatatcctc cctaatgttt aaaaatataa
     7861 ttaaaaaata agtattgtgg ttgatcttac aatataaatt ctgcttgtcg atttaagacg
     7921 tgtccacaat atgtcctaaa aaaagtataa aataaacttt ttcctgtgtc aatgcagttt
     7981 tctgaatttt ctggttgagt ggtgttagtt aaatatgctt taagtagatt aatgacgaat
     8041 tatatctaat gtatttaaat agcatatgca ctttaaagtt aattttaaaa caaaatttta
     8101 tgaaaagcta gtggaagatt aagttattaa aataagtagc gtagtaaagt gttagatttt
     8161 ttaattaatc aaagaaattt actttgtatt ttctacaaaa atctgtgaat atcttttgaa
     8221 ttatgtagag gatataaatt tattttattt acatagatta gattttgttc taatttactg
     8281 tattcaaatg gttttccatg tccaggataa atcattttag gttttgtatt gatgattgtt
     8341 ttccaagatt gaataaattc tagtttgctt tctgcaaata tggtaatttt gtgagtactt
     8401 ggaaatccat tcattgcaga atctccacaa aataaaacac catcatcaat tagtagtgaa
     8461 attgaatcag cagtatgtcc tggtgtatct ataatttttc catttagttc tttttcaatt
     8521 tctttacgat tcttgtcaga cacaagaata cacctttttt caaattcttg tttaagtgga
     8581 gaaaaactat gctcaccttt tccaaatagt ttcataattt ggcaaaagag aaaggcagta
     8641 cggctgttac aaccaccaat aaaagagttt tgacctttat atagaatttc taaagcttta
     8701 tcatgcataa caatctttgt attaggagat tcgtctagaa tctgatttag aaaaccaaca
     8761 tggtcatcat gagcatgagt tagaaatatg tatcgaatat ctttgatttt tatgttgaat
     8821 ttagaaattc gttttttgaa atgtttaaaa ccattttcat atccagtatc aatcaagaca
     8881 aagccatctt gaatagggta caaccatgta tttataattc gatttcctgc attaaacatt
     8941 ttttcaccac cttgaattag aatataaatt tatattaatt agaaagaata tagcatagtt
     9001 ctactgatat tttaacgtat ctctaaaagt tttaaggagg ataatatatt ttagtttaat
     9061 ctaaaagcta aaagaaaaga agtatactgt tacaaaaaca aagagtggta taacttttac
     9121 agtagatacc accctattat aaaaatagac ttataaagtt ttatcgtgtt tttctaggaa
     9181 tgttagatat tcttctttag tcataactgg agtaaaggta tctaatatat ctttagcatc
     9241 cttagcatta tcaaaaagaa ggtctacaac tgtaagtgcc attgctttag caggaattat
     9301 gtaagctaaa tcttcatcaa ctatttcaaa atctcttgta tgaagagctc cttttacacc
     9361 accaatcata gggtgaagag taggcattat atgagacaca tctccaaaat caaaagagcc
     9421 tgtaaaatct ccaccatcaa taacttttcc ttttagacct aaatcttcaa gattgttttt
     9481 aaataaatta tcaagacttt tgtatcgtaa tattggaaga tatccaggaa tttcagttat
     9541 ctcaacatca gctccaactg ccattccacc agccatcaaa gccttattta ttctttcatt
     9601 agcatcaatc ataccattta tagttctagc tctaacatat gattccatat gaacatcagc
     9661 aggaacaaca tttactatat ctccaccttt tgtgatgata gggtggaatc taactctttc
     9721 agactcttta aaagtttctc taagtgcatt cacgttgttt atagcaagca ttgctgcatt
     9781 taaagcattt attccatcat gtggagcaga accagcatga gattctttac ctataaattt
     9841 aacctttttt ccaacaaatc cattactctc aggaccaact agagccttat cttctcctaa
     9901 atcagaagca tgaaacatca tagatatgtc tatatcatca aaataacctc tttttattaa
     9961 ttcttgtttt ccaccaaaat aagttatttt tccttctttt tttaattgct ctctgtattc
    10021 caactcaata aactcttcag caggagtagc cataaaagat actttaccat ctaaatgttc
    10081 taaaacgcca ctttttacta aaccaacagc agcacctaac attccagcta tttgaatatt
    10141 atgtccacaa gtatgagaag cacctatttc attagcatct ttatgttctt tacatgatat
    10201 accatccaat tctccaagta tagatatatg aggacctttc ttgctagaat ttgcatctgc
    10261 tttgcatcct gtaactgcta tatttttttc tacagataaa tttaattctt gctcaaaaaa
    10321 gtttgaaaca gcttctgtag ttttaaattc tttatatcca tattctggat ttttgtatat
    10381 tccttgacct atttttaata ttttttctct attttcatct atagttttta ttactagaga
    10441 ttttaattgt tctttattca taataaattc ctcctatatt ttaccttgaa tacttaaaac
    10501 tatatgagct atactcgcac atgcaaagaa tatagttgta aatacaataa gtgaaattat
    10561 aactattttc catgacattt cttttaaagc ttctattttg tttccaacag aaattcctgc
    10621 aaaagctagt agtggagtag ttatagccat aaagttaata ttattagtgt tagttaagaa
    10681 tacttcagat gttggactat atggcatact aagtaaaagg ccgattatag ttgcaaatcc
    10741 aaaagctgga aagattgatt taggaaataa atcttttata atcatagctg ccatagcagc
    10801 taatattagc atcaccatac caggaagagc tgtaactata gatgtaccaa cgctaattct
    10861 ttgtcctata agaattataa ctccaataac agacataacc aataattgat atgaactttt
    10921 ctttaaattt aaattagcaa acatttatta gcactctcct tctgtagatt taatttcttt
    10981 agttttttta tttctcgaaa atttaggttc taaaatctta tacataaaat tagtaaatgg
    11041 taaggataca aaaactaaga atataattcc tgttatacta gacatcatat tgcttgttgc
    11101 agcataggca agtattgtat ctgccatttc aggtggtacc atggtagata atgaaccagc
    11161 agctgctgtc atcatacttc cactaccaac accacaggcc attcccaaag catatgggtg
    11221 aagaccacta taaatagata ttgaacctag tacactaaag tatatagtac caagaactgt
    11281 tcccattaaa tatgtaccaa gtacaccact accttctggt gagtttatcc catatttttc
    11341 tgatataact cctaaagaag gttcacgact tatacttgca gtagctccaa cagcttctct
    11401 tttcattcca aacattaatg caagaggtaa tgcaacgatt ggtgcaagta tatgaccaaa
    11461 ttcttgagcg ataaatgctg gtccagcttg aattattttg tcaatgtttg gtccaactgt
    11521 agtaccatat tttataccaa gcattactag tgcaattcca acaacttcac cagcaatatt
    11581 tatttctttt tctccaacca cttttttaag tgctttaatt tttttaccta ataaatctgg
    11641 tgttattata agtccaatta taactgcata aagcataggg aacagtacta tagaggcttt
    11701 tccaatagct atagttcttt gccctataaa ttcacatatt actaccaaga ttaagcaagt
    11761 taaaaatact tgaataaaat gttttttatt aatttccatg atttcctccc caaaagaata
    11821 aatattttac ttatctgaaa tgagaaaata cgttttctta agtataatac gttttcctaa
    11881 ataaaatgaa aaaactattt caaataagtt tcagaaatgt caataacatg tccttaatta
    11941 aggataaaac aaaaatttaa cattgtcaac gaaaaatatc taaaatatta catattttga
    12001 atatattgta atatagcttt attatgaaaa aattgtttaa atattgtagg aataatagct
    12061 aaaaagcttt aaaattagta atataaaatt gaaaaataat aatatagagt tgagaaatag
    12121 taaattgaaa ttttaaaata aatgagtgga agtagcttag taaagtatgg aaatgaatat
    12181 tacaatagaa aataaataat atgtaaagac tttaaattaa ttttaagtat taagtacaaa
    12241 tactaatttt tgatatagaa attgaataaa gtaaatgata ataagcaaat gaattaagat
    12301 gataataagt gaattgatat agaaataagt aaattaagta agatgataac aaatgaaatg
    12361 ataataagtg aattgatata gcaataagta aattaagtaa gacgataaca aatgaaatga
    12421 taataagtga attgatatag caataagtaa attaagtaag acgataacaa atgagatgat
    12481 aataagtgaa ttgatataga aataagtaaa ttaagtaaga tgataacaaa tgagatgata
    12541 ataagtaaat tgatatagta ataaataaat taagatgata atatccaaag tgagaaagat
    12601 aatagaaagt aaataaaatc atgtaagaat aatttagata agattctaag attttattta
    12661 tttaaaaatt agtatgatta atttagatta aataaattta ataattttag taggtggtaa
    12721 tgaatgaaaa taaatagatt aacagaaata atagtaattc tattaaataa aaaattagta
    12781 acagctaaag agttggcaga taggtttgaa gtttctacaa gaaccatata tagagatata
    12841 gaaactctgt ctatgtcggg agtaccagta tatatgacga aaggcaaagg aggagggata
    12901 tctttaatag aagaatattc tatagacaag gccatactgt caaaaaaaga taaagagagt
    12961 ttaatagttg cactgaaaac attacaggcg actaagtatc cagaaataaa ttcagtagta
    13021 aataaaattg gctcaatatt tggagagcag aatttttcaa actggattga aatagacttt
    13081 actgaatggg gaagtaattt taatgaagat gataaattta caaaaataaa agaagctatt
    13141 ttaagacgta atactataaa ttttaattat gtaaatagtt tgtcaagcca gacaaacaga
    13201 actgtcgaac cactgaagct tatgtataag agtaagactt ggtacttgta tggattttgt
    13261 aaactaaaag atgattttag gatatttaga attagtagga taaggaattt atctataaag
    13321 gatgaagtat tttcaagaaa gataatagaa gaagtatgtt tgaatgattc taaagtaatt
    13381 aaggaaaata ctataacgtt gaaattgagg tttaaagaaa agatgctttt tagagtattt
    13441 gatgatttta ataaggattt aataactaaa aatgaagatg atacatatga tgtaattaca
    13501 gaatttccta ttggtgaatg gatttatgga tatattttat cgtttggtga caatgttgaa
    13561 gtacttgaac ctaaagatgt tagggataat gtaataacta gactaaggga gttgtctaaa
    13621 atttattcat tataatatga tgctagatta aagattttat taaatatata atatcttgca
    13681 tttagtatat aatatctcac tgagtttaat tatattaaaa atatgacata aaggtgtcgt
    13741 atattataaa ttataattga attagttcaa aataaaatat attaataaaa acaaagtgag
    13801 gtataaagta tgaattatga aatagtagag ataaatgaga aaatagttat aggaatatcg
    13861 aaggagacta caaataaaga tggacaagcg gtaaatgcta taggtgaatt gtggaaaaaa
    13921 tttatgggca aagggatata taatgctata aagggtaaaa agaatgacaa gacaataggt
    13981 ctatatacag attaccaggg tgattttaca agtccgtata gctttgttgc atgttgtgaa
    14041 gtaaatagta attctgataa agagaagaat ttagaagaat taaacactaa taatactgtt
    14101 ggagaaagta ttattagtaa agttatacta gcaggtaagt atgcaaagtt tgttatagta
    14161 ggaggtcaaa aagaagtagg agatttttgg tttaagtttt ggcaaatgga ttttgatagg
    14221 acttatataa gtgattttga agagtatcaa tgtaatactt ttgatacaaa gaaacaagaa
    14281 attcatattt acataggaat aaagtagatt tgttattact atattgaatg aagtattgag
    14341 tatagtacat ttattataga tagttttact atttataact ataagcagta aatctgtaaa
    14401 agatttactg cttttttagt aattaatagt atcagtatca ctt
//
LOCUS       DAEPLF010000077        14099 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEPLF010000077 DAEPLF010000000
VERSION     DAEPLF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14099
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.077"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(88..1197)
                     /gene="ugpC"
                     /locus_tag="KR314_002006"
     CDS             complement(88..1197)
                     /gene="ugpC"
                     /locus_tag="KR314_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBH0328389.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDKIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            1456..2193
                     /gene="cas6"
                     /locus_tag="KR314_002007"
     CDS             1456..2193
                     /gene="cas6"
                     /locus_tag="KR314_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBH0328390.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            2198..3673
                     /locus_tag="KR314_002008"
     CDS             2198..3673
                     /locus_tag="KR314_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328391.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKKMIDNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            3693..4706
                     /locus_tag="KR314_002009"
     CDS             3693..4706
                     /locus_tag="KR314_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBH0328392.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIIFGKKGNIYPSIKQNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEKEENEVLR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDKLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            4722..5528
                     /gene="cas5"
                     /locus_tag="KR314_002010"
     CDS             4722..5528
                     /gene="cas5"
                     /locus_tag="KR314_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBH0328393.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIKKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVMPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCNVCIDTKEVFTWL"
     gene            5555..7963
                     /locus_tag="KR314_002011"
     CDS             5555..7963
                     /locus_tag="KR314_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBH0328394.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLLEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQEEIYDYIE
                     KGILEKEEFNLKDIESYINDILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDDMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEGALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     NYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            8404..9351
                     /gene="prmA"
                     /locus_tag="KR314_002012"
     CDS             8404..9351
                     /gene="prmA"
                     /locus_tag="KR314_002012"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBH0328395.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQDGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            9370..10128
                     /locus_tag="KR314_002013"
     CDS             9370..10128
                     /locus_tag="KR314_002013"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBH0328396.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            10130..11428
                     /gene="mtaB"
                     /locus_tag="KR314_002014"
     CDS             10130..11428
                     /gene="mtaB"
                     /locus_tag="KR314_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBH0328397.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            11471..11821
                     /locus_tag="KR314_002015"
     CDS             11471..11821
                     /locus_tag="KR314_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBH0328398.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            11984..12163
                     /locus_tag="KR314_002016"
     CDS             11984..12163
                     /locus_tag="KR314_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBH0328399.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            12193..12636
                     /locus_tag="KR314_002017"
     CDS             12193..12636
                     /locus_tag="KR314_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBH0328400.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            12747..13244
                     /locus_tag="KR314_002018"
     CDS             12747..13244
                     /locus_tag="KR314_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBH0328401.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            13437..14000
                     /gene="raiA"
                     /locus_tag="KR314_002019"
     CDS             13437..14000
                     /gene="raiA"
                     /locus_tag="KR314_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBH0328402.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
BASE COUNT         5808 a         1326 c         2503 g         4462 t
ORIGIN      
        1 gatagatatt tattttttta ttaagctgtc tttctaaaaa atcttttaac tcttccataa
       61 agtctcctta aaaataagat tatattctta tcttatagct aaagtagttt ctttgtcaaa
      121 tatgtgtact ctactattgt caactgaaag tgttaagtca tcaccaacat taattctgtt
      181 tttactgtca aatctaattg ttatcgtatt ttcgcctagt tttaagtgag cataagtttc
      241 tgctcccata agttctttta tttcaattct tgatttaaaa caagtatctg gattagattt
      301 aataaaagta tcttctacat gaatatcttc tggtctgata cctataacaa cttcattatt
      361 aatataattg tcttttatta aatcatagtg tttttcttta ttcaatttaa gcttaatatg
      421 ttcattttgt gcataaattt tatcattttc ttctattaat attacatcaa taaaattcat
      481 ctgtggtgct ccaataaatc ctgcaacaaa tatattagct ggataatcgt atacatcttg
      541 tggagttgca atctgctgaa ctactccatc cttcattact acgattctat cagccatagt
      601 catagcttca acttggtcgt gtgttacata gataaatgta gttccaagct gttggtgtag
      661 cttagttatc tctgttctca tagcagttct aagttttgca tctaagttgg atagaggttc
      721 atccattaag aataccttag gatttctaac tattgcacgt cctaaggcaa ccctttgtct
      781 ttgtcctcca gataatgttt taggtttttt atttaaaaga ggtagtaagt ctaatatttt
      841 agcagcttct ttaacttttt tgtctatttc atctttaggc aattttctaa gtttaagtgc
      901 aaatgccatg ttttcgtaaa cagataagtg gggatataaa gcatagtttt gaaaaaccat
      961 agctatatct ctatcttttg gttctatatc attgacaagt ttatctccaa tgtatagttc
     1021 tccttcagag atttcttcaa gaccagcaat cattctaagt gtagtcgatt taccacaacc
     1081 agatggtcct actaaaacga taaattcttt atctttaatg tctatattta tattttttac
     1141 tgcgttaaat ccatttgagt aaagttttga tatatttttt aaaattactt ctgacataat
     1201 attctccttg atatttaaat tttaatgata tagtatcaaa tttttttatt atattaaatg
     1261 tgtaaaatga ctaaatgtat ttgtttaata tgtatattta atttttagca aagaataaaa
     1321 ctaacagtaa aatatattta aaaaattaaa aaacaaaatt aaatttatta tcaaaaaatc
     1381 attttatgat atgttttatt ttttatatta caaatagaga ataatgaatt atactagaaa
     1441 gtaggaggtg aaaatatgag cagaatttca atttttatgg agtcatgtaa tggcaaattt
     1501 ccaaatgaaa attcaatgtt gtcagtttcc tttataaaaa atattttaaa tgcagaaaac
     1561 aaagattttg ctaaaaatat atttaactat ggggaagaaa gaaaaaacaa taagcaaata
     1621 aaggatataa atacagccat ctacatacca aatcttataa gaagcaaaaa tgagttgctt
     1681 gttgatggag atataatatt caatataagt ttttataatt attctatgtt cagtaaatta
     1741 tacaatggaa ttttaaaagt aaaggagcta gaatataaag gttatagatt taaaataaaa
     1801 aatataaaaa tacataaaga acacgagata aaagaaaatg gtgttatatt taaaactatg
     1861 tctcctataa tagtaaagaa taaagaaggt aaatatttag atgtagaaga ttcaaattat
     1921 atagaatgtt taaactatat agctaatcta actctggaga gtataagagg tagtgggctt
     1981 agaaagccat tggaatttat acctcttaac ttcaaaaaaa gagtgttaaa agaaaagatt
     2041 cgtggattta aagtgagaga atattactat ataaatgctt atgcaggaac atttttttta
     2101 aaaggaaata tggaagattt aaatgcactg tataaaatgg gaattggata tagaaggacg
     2161 gaaaatgcag gaatggttga tatattaaag tagcgaggtg agagaaataa aagctaaatt
     2221 ttatagatca ggtgatattg taagagatat tggcattgtt tacttttatg aattactaat
     2281 ggatttaaaa aacgaactag aaaaaaatga ttataagcta aaatttactt gtgagttaaa
     2341 tagaaattat ttatctttag aatctgcaga atatatagac cctaagtata tatctgatta
     2401 tatattagaa aatcaacttt ttaaggtatt taaaaatggg aaaaaggaaa ccttagaaaa
     2461 aatatttagt aatatagata atttaacgta tgtaaatttt attgaagaat tagataaatc
     2521 ttctgcaact gagaaacaaa aggaagggat taaaaaagat tttaaaaaca gaagatttcc
     2581 atatgttaga aatagtggta agtatggatt aaacacgagt ttagaaaata tggaaacaaa
     2641 tgtaaaaaga atagtagata ctgtaattaa atcaaatata gagatagatg aaatagataa
     2701 acttgatttg acacaatatg aagaaaaaga tagtgtatgt aatgtttgta atataaataa
     2761 aacaacaaaa ctagatattg atttaagaga gcgaaaggac tctaagtata actttttatt
     2821 tagaggtgca gaaaaatcag gctttaaaag aagtggacaa gttgaaagta atatttgttt
     2881 tgagtgtgaa tttttcaact taatgtgtct tttatatata aatttaaaaa gaccaatggt
     2941 ttttgcatat acgaatgatt taagggaatt agcattttta aatcataaga taatgcttaa
     3001 aagaaagatg tattcagata aatcatttta taaaaaactt ttgcatgaaa aaataagttc
     3061 gattagacta tataggtttg atattgatac gaataaaggt ataattctaa agtttgactc
     3121 tattatagag tataaagaat tattaaaaaa aattgaactt atagatatta tagataatta
     3181 taatttttca agagagccag ggaatactag aaatttgggt aaaaaaatga ttgataatgg
     3241 gaatttatca aatttaaaag aattattact tgataatata agtgttttaa ggcaactaac
     3301 tggaacttct cgagaaatgg attttgtatc tagctcatat aatatcggtt tatatataaa
     3361 attgtgttgg attgtagatg gaggggagga gtataaaatg aaaaactata tggaaagtaa
     3421 tttaatatat tcaagagtag gtaaagactt atttaataaa atgacagatg aatccagaag
     3481 aaaaaatttt tctatgaaag tgattcaact acttaagtct aatgatagaa ctcagctatt
     3541 tcaaactata atgcatgtgt tagtttctaa tggaatctta ataggagaag ggtttgtaga
     3601 aggtctaatg cagagttctg aattagaatt aaatacgaat gtaggtctat ttattcaaga
     3661 aatcatgaaa taaaaaaggg ggaaaattta aaatgaatga aaaaagagta aaaggagtaa
     3721 cattaactat actgacagaa agcccagttc cactttcata tgaccaaggg tatggaaatt
     3781 atacaccaat aaaaaaagaa caatatagag aaaaaataca tgcaaaaact agtatagcca
     3841 caataacata tgatttaaga agaatgctcc accaagaata tggttggaat ttaagtaata
     3901 ttatttttgg gaaaaaagga aatatatatc catctattaa gcaaaatgta ggatgtgtag
     3961 atgaaaatgg attagagact gatgtatttg gttatttgat accacttaaa gataaaggtt
     4021 ctataagtaa ggcttctccg cttagaataa taccttttag gtcattaaat tcatatagag
     4081 gttcaacaca gttgataaca aatagaggtt ttttaagtag cgaatttggt agaaaatatt
     4141 atgatgagaa ggaagaaaat gaagtactta gagatgaaaa ctttccaaca tcacaagcat
     4201 tagcaataga agagacttta tcagattact acgtttatac aataacattg gagcttgata
     4261 gaataggagt agtagaagta gaagatggaa agttgctttt accagaagaa agaaaattta
     4321 tgagtaagga gttaagagaa aaagcagtaa aagatatatt agatgcaatt actgtgttta
     4381 caagaaacat aaaacattca tcagtactgt taaaaccact tgcagtaatg ggtggagctt
     4441 ttgataaagt tgtaccgttt ttttgggatg atgtagatta taatgctgat agtggtgaga
     4501 taaatttaga gggagtcata gaaactatag aaagttatag ccttaaagaa agtaacacaa
     4561 tacttgctat caatgacaaa ttaaaaattt ctaataaaaa ggaattaaat aagtttaatt
     4621 taagtaaata ccctgtaaaa gagattaaaa atctagcaga tagattggaa attggagaag
     4681 ataatatgtg gtatttaaaa gagtaggagg ttttttgaat tatgaaagta ttacaatgta
     4741 aacttaagca accaactgct cactatagag acccaaaagt atttcaaaat gaatatataa
     4801 gtactttaaa tttaccatct aagaccacaa taatgggaat gataacgtat ttatgtgata
     4861 gaagattaaa ctctgatata gatataggaa taattggaac acatcatcat agagaactag
     4921 aattttcaag aggagagaac attgattttt ggaatgaata tacaaatatg agaaaaggca
     4981 aagataaaga aaagtttttg cttcaaggaa actactatga ttactataaa gaacataaag
     5041 ctcaaaatag catattaaac tatgaagttc ttaaagaagt agaactgact atatttattt
     5101 cttgcaagga tgatgaagaa cttgaattta ttaaaaaaaa gcttgaaagt ccatgtaaat
     5161 atgcaaattt aggaagaaaa gaagattttg taataccaag tgaaaaaggg tgttttgtga
     5221 aagaagttga gttaaaagag gttatgccta tgaatactcg tgatgctata aaagaaaata
     5281 taaaattaaa gaatacatat gtaagagttg acttaagaga taaagagaat gtcgagacta
     5341 taataaatca aggtgtattg attgcattac cttataaata taaagattta gaagcaaata
     5401 gagatgatag agtttatgag ttttgccatt atatatatgt tgataatgat ggtatatatc
     5461 ctaaaaatat taaggtaaat gtatgtaatg tatgtattga tacaaaagaa gtttttacat
     5521 ggctttaaat tattttgaaa gataggtgtt tatcatgaaa aatatcatgc aaaaagaaat
     5581 attagctaag tcatataatt taagaactaa tcaacaagaa caaacattag ttgaacatat
     5641 taaggattta tttgaagtat tagaatctat attagaacta aatctatatt cagataaaga
     5701 tgtagagata ctaaaaatat gttgtgcttt gcacgattta ggaaaaatta attcaattat
     5761 gcaacaaaaa atggagatta ataataaaat atcgtatagt tgctcagaag aagagaggaa
     5821 aaagttagag tctgacaaaa aatctttaaa aaagatagca agacacaata tattttctgg
     5881 agcattttta aaagatatat tagaaaaaat gaacttgtta gaagaagata agttatatat
     5941 atataaaagt ataatgttac atcatggaaa ctatgaagat tatatgaggt taagtactag
     6001 taaagttcaa gaagaaattt atgattatat tgaaaaaggc attttagaaa aagaagagtt
     6061 taatctgaaa gatatagaaa gttatataaa tgacatttta aatgtagatt ttaaatttgg
     6121 tgaagatgta ttggattatg attacataga taaattaagt gaaagtatgt ttatagacag
     6181 tgattataac aatggtcaaa tagatgattc agttttaaat tatagaaagt ttaagtatat
     6241 tttatataaa gggatgctga acttaataga ccatagtgca tcaagtagac agaaaggtat
     6301 aaaattttat aacgatttta ctgatgaaga aattgatgat atgattttaa atgagatata
     6361 taagagtcag ggtaatttag aaaaaaataa tatagagttc aatactatac aaaaaaggtt
     6421 gagagaactt tctggaagaa atgttctgac tgttgcattt acaggttcag gaaaaacagc
     6481 tgcagattat agaaagactt ttaaaagaaa attcttttta gtgccaaata aaatatcagc
     6541 agaaagtttt tatagaaaaa atatttttca aaataaaaat gatttagata caagaagtag
     6601 taatgattac attggattac ttcatggaga tataaactta tattcagaga atgaagataa
     6661 tggagaacat gattttgtat tgacattaag ggatatagat ttgagtataa atttttgtaa
     6721 accttgtgta attgcaacag ttgaccagtt attacttagt atgtttaaat ttccgggata
     6781 tgaaaaaata ttcgcagccg taaaaaatgc atctataact gttgatgaag tacatttatt
     6841 ggaaccaaag atgtttttga ttttgatata ttttatgcag tttgcatgca agtacttaga
     6901 tgttgatttt cacttaatga cagctacact tccaaaatca tataaagagc aaatgataaa
     6961 caagggaatt atttttcaag aagaaagtaa tgaaaatgtt actgataaag gagaaatagt
     7021 ttttattgag agcaacaagg aagaagaaat ttgtgaaggt aaagatgtaa aggtttcttt
     7081 tataaaggaa aaagaaatca agtcaatagt tgaaggtgct ttagaaaata aacaaaaaat
     7141 tcttataata aagaatacta ttgattcagt caatagaaca tatgagtttt taaaagaaaa
     7201 tttatctgat aagtatggtg gtgtagatat agatgtttta catagtagat ttaagttcaa
     7261 agataaaaaa gagaaatata gtaaaatatt aaatggagag ggtgatattt ggatatcaac
     7321 tcaatcagta gaaatatcct tggatttgga tttcaatata ataataagtg atttagcaac
     7381 tatggatagc ttaatacaaa gaatgggtag atgcaataga aataataaat atgaatatgg
     7441 aaacttttat attctaccta gtgaagataa aatttatgat aacaaactta agaacacaac
     7501 taaaaacatc ttaaaggaca tattaaaaac taactctatt tttacaatgg ggattagaaa
     7561 aactattttg gatgattact atgataatag tgtagttaaa aactattttg aagagaattt
     7621 tataagttgt gataaagaaa ttaagaatat ttatggtata aataaagaaa tttttgatgg
     7681 attggattta atttttaatt ttgaacctta caaaaatatt gttgatagta agaaggaagc
     7741 agctaaaatt tttagagatg ttgatgttag ttacaaaatt attctggaag aagattttta
     7801 taaggaagat agggagttac aacaagattc gattcaagta tcagggttta tatttaatag
     7861 attatattat tatggattaa taagcaaagt agaaggatac atggtactaa aatctagtag
     7921 taagtttgag tataatagta ctgtaggctt aattttgaag tgagcatatg tttaaatata
     7981 atataccttt agacattgaa aatacaaaaa gtactactgt aagcccactg taaaaactat
     8041 tttttaaaag gaaaaataag ttgtgagata gagtgtctac aagtgtatat agataataaa
     8101 tttgaggttt aagattaact aagtgggttt caggtacatc gcgaaaataa ttataagttg
     8161 gagattttgt atgtttaaga ttaactaagt ggatttcaaa tattgatata gtagatataa
     8221 tagaagtttt attattaggt ttaaaattaa ctaatcggat ttttaaatat tctgactatt
     8281 atataaggat attattagct tgaatttaaa accaataata taaaacaaca aaatattata
     8341 tttatgatat aataaaagtt atggaaaaac aggaatatta ctatagagaa gagaggtaaa
     8401 cttatgaata attggataga agtaactata aaaacaacaa cagaagcagt tgaacctata
     8461 acgaatatac tatatgaaca aggagctgga ggagctgtaa tagaagaccc aaaagacttt
     8521 ttattccaaa agaaaaatga actagactgg gattatgttg aagaagaagt atttaaaaag
     8581 aatgaagaag atgatgttct tataaaaact tatgtttctg aagaaaaaaa tgtgatggaa
     8641 tttgtcgaaa taataaagca aaaagtttta ggattaaaag attttggtat agatataggt
     8701 gaaggaagtg tatcattgga ccaagtaaat gaagctgatt gggcaaatgc atggaaagca
     8761 tactacaaac caacaaaagt aggacaaagg gtagtagtaa agcctacatg ggaagattat
     8821 gctatgcaag atggggattt aatcatagag cttgaccctg gaatggcatt tggtacaggg
     8881 acacatgaaa ctactagtat gtgcattaga gaattagaaa agtatgtgaa taaagattct
     8941 aaagtatttg atattggatg tggtagtgga atccttgcaa tagcagcagc taaattagga
     9001 gcaaaagaag ttgtagctgt agatttagat gaagtggctg taaaagttgc aaaagaaaat
     9061 gttcttgaaa ataaagttga aaaaagtgta tctgtaatgc atggtaattt aactgatgta
     9121 ataaaagata aagcggatgt aatagttgca aatataatag ctgatataat aaagatactt
     9181 gcaaaagatg ttcaaaactt tatgaaagaa gatgctatat ttatatcttc aggtataatt
     9241 ttggataaag tagaagaagt taaagaaagc ttaattgaaa atggatttga aatagttgaa
     9301 gttcaaaaat taggtgaatg gtcagcaata gtttctaagc ttaaaaaata agggatatgg
     9361 tgacgtataa tggatagatt ttttgttgaa aaaaataata taaatctaca agataaaact
     9421 tgcactatag aaggtgaaga tgtaaaacat atttcaaaag ttttaagatg caaactaggt
     9481 gagaaacttg aaatatgtga caagaataat aatgagtata tttgtgagat aatgaatata
     9541 gataagagta tagttaattt agagatttta gaaaaagttg atataaatag agaatcagaa
     9601 cttaaagtaa ggttatacca gggacttcca aaagctccaa aaatggaaat gatacttcaa
     9661 aaattaactg aagtgggtgt agaagaaata atattagttc aaactaagag aagtgttgtt
     9721 aaagttgatg acaaaaaaga agataaaaaa tttgaacgtt gggaaagaat aatttatgaa
     9781 gcagcaaagc aaagcaaaag aggtaagata ccaaaattaa gaggtgtttt aagttttaaa
     9841 gaagctttag aagatatgaa aaaaaataat gtaaatattt gcccttatga aaatgaaaga
     9901 acagtttcaa taaaacatgc actaaaaaaa tgtgattcta atatagatag tgttggaatt
     9961 tttataggtc cagaaggtgg attttctgaa gaagaaatag agcaaataca aaaaaataat
    10021 tgtaatgttg tatcattggg gcctagaata ttaagaactg agacagcttc tgttgtagca
    10081 tcaacaatag ctttgtatga actaagtgat ttaggaggag aaaaataaat tgaaaaaagt
    10141 agctttttat acacttgggt gtaaagttaa tcaatatgaa acagaagcta tgctagaact
    10201 atttgagaaa gatggttatg aacaagtcaa tagtgaagag tatgctgatg tttatgttat
    10261 aaatacatgt acagttactc atatgagtga tagaaaatct cgtcaatata taagacgagt
    10321 taaaaagaag aatccagatg caataatagc cgttgtagga tgttattctc aggtatctcc
    10381 agaagaaatt ttggatatag aagaagtaaa tttagtcatg ggtacaaatg atagaagaaa
    10441 gattgttgaa gaaattaaaa agataaattc aagtaagaaa gttagtacag ttgatgacat
    10501 aatgaaagtt aaagcttttg aagaaataga aatcagccaa actaatggaa agactagagc
    10561 atttatgaaa atacaagatg gatgtgacag attttgtact tattgtatca ttccttatgc
    10621 ccgaggacgc gttagaagta gagatattga tagtatagtc gatgaagtga aaaaattggc
    10681 taataatggc tataaagagg ttgtattaac aggaatacat gttgcctctt atggaaaaga
    10741 tttaaaggat agggatatta aacttcttga tgtaataaaa caaataaacc agatagaaaa
    10801 aatagaaaga attaggctta gttctgttga accaatttta tttacagatg aatttgtaaa
    10861 tgaagtttta aagatggata aagtttgtcc tcattaccat ttatcacttc aaagtggatg
    10921 tgatgaaact ctaaaaagaa tgaatagacg atacacaact ttagagtaca aaactattgt
    10981 agatagattg agaagtaaaa tgccagatgt ggcaataact acagatgtaa tagtaggttt
    11041 tcctggagag acaaatgaag agtttaaaaa aacgtacgag tttttaaaag aaatagagct
    11101 ttcacaaatg catattttta aatattcacc aagaaaagga actccagctg caactatgga
    11161 aaatcaagtt gaccctcaaa tgaaacactt tagaagtgaa caacttttaa atttaagtaa
    11221 agtaaatttt aataaatttg caactaaatt tatcggccgt gagctggatg ttttgtttga
    11281 acaaaatata gaaggcaata aatatgaggg attaacttca aattatataa gagttgttgt
    11341 tgaaagtgac aaaaatatac aaggtcagat attaaaagtg aaaataaatg atgtaaaaga
    11401 tgaatatgta gaaggaattt tgctataaca tgtagaaatt ataatattta taagatagga
    11461 ggtggaaaga atggattgta ttttttgtaa aatagcgaac ggagaaatac cttcaactaa
    11521 agtttatgaa gatgatagag tcttagcttt taatgacttg aacccagttg ctccatatca
    11581 tatattagtt gtacctaaaa aacattatga tagtttaatt gatattcctg ataaagagat
    11641 ggacattgtg tcacatattc atgttgttat taataaaata gctaaagaga aaggttttga
    11701 ccaaactgga ttcagagtca taaataattg tggaagtgat ggagggcaag aagttaaaca
    11761 tttgcactat catattttag ctggtaaaaa gcttcctaat tacgaagcgg gacaaaacta
    11821 aactaaatta ttgaaaaatt gagttccttc ttgataaaaa tacttgataa aagtaaaaat
    11881 ttagtttata ataaacaaag tgttaggttg taatgcatat tatgcaaaaa gaacatagtt
    11941 acagtcccag cataattgcg ctattggagg gagggaaaaa gaaatgtcag aagtaagagt
    12001 tagagagaat gaaacattag acagcgcttt aagaagattc aagcgtcaat gtgcaatgtc
    12061 tggcatcatg tcagaagtga gaaaaagaga gcactatgat aagccaagcg ttaagcgtaa
    12121 gaaaaaagct gaagcagcaa gaagaaaaaa cgctaagaaa tagtagtaaa taagaataaa
    12181 gaggtgaagg ggatgtccct taagcaaaag ttacaagagg atttgaaatc ttcgatgaaa
    12241 aacaaagata cagtgagaaa atctgtagta actttaataa gagcttctat aaagcagtat
    12301 gaggttgata atagggtcga gcttgatgaa gatggaatta tcgatgtaat agcaaaacaa
    12361 ttgaagcaac gtagagatgc attagttgaa tttgaaaaag ctggaagaga agacttaata
    12421 aaagaaacag aaggtgaaat tgaagtttta aaagagtacc tacctcaaca attaagcgaa
    12481 gaagagttag aagaaattgt aaaatctact atatccgaag taggggcaac ttctatgaag
    12541 gatatgggaa aaataatgtc agttatccaa ccaaaagtta aaggaagagc tgacggaaaa
    12601 cttataaata agttggtaaa acaaaactta caatagattg atatagccta gagagaaatc
    12661 tctaggcttt ttatagtttt tgggaataac gagtcatatt tgagcatatc tataatcaaa
    12721 gaaaattttg aaagaagggg aaaaatttga ttatatcagt aaaagagatt aggaattttt
    12781 tgataaacat atttatacca ctaataatag gttacttaag tagcatctta acaatgatta
    12841 tcagtggaac agatatttca acctattatt tacaactcac aaagccaagc tttgcaccac
    12901 cagcttttat ctttcctata gtttggacta tattgtacat attgatgggt atatcttcat
    12961 acctaatatt aagaaagggt tacaacttac ctaaggtaag agatgcaatt ttttattatg
    13021 gattacaact tgtattaaac tttatttgga gtatactatt ttttggattt ggacttagat
    13081 ttacagcttt gatagatata ataataatga ttttggtttc tataataatg atttcgaagt
    13141 tttctaaaat agataagaga tctggataca taaatttgat ttatttaatg tggcttgttt
    13201 atgcaggatt tttgaactat tttatatggt ttattaataa atagaatgta aagattttgt
    13261 aaattaccct gtacttttaa taatatcata acaaacgaaa aataaataga ctattaaaaa
    13321 taatcatttt catatacaat aatattaatt taaatacaat aaatttataa aaattgtatt
    13381 taaagattaa catattaaag tattagaatt ggaaaggctt gaagggagag gtttttatgc
    13441 aaataatagt atctgggaga caaatgaaat taactgacgg aataaaagga tacgtagatg
    13501 gaaaattaag taggttagaa aaataccttg atcctgaatc agaagtgaaa gtaacagtaa
    13561 gtgcgaaaaa ggatagacaa aaagtggagg ttacaataat tcctattaac ggtcaaataa
    13621 taagagcaga agatgtagaa gatgacttat atgcagcaat tgatattgtt tgtgacaaat
    13681 taagtagaca agttgtcaaa tataaaacta aagttaaaga caaagttcaa aataataaga
    13741 gcataagatt tgaaaatctt gactttatag ataatagcag tgaatttgat gattatgatt
    13801 atgatgatga agaagatgaa aatatagtaa tagaaagaag aaagaaattt aatgtaaaac
    13861 ctatgagttc tgaggaggcg atacttcaaa tggaactagt aggacacaat ttctatatgt
    13921 ttagaaacca agataatttt gaaataaaca tagtgtataa gcgtaaagct ggtggatatg
    13981 gtttaataga acaagattaa taaaaattgt atacgtttag attttatata agaaaaaata
    14041 aaaaggtaat catgttgatt ttttatcaat atgattacct ttttattgtt taaataata
//
LOCUS       DAEPLF010000078        14098 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEPLF010000078 DAEPLF010000000
VERSION     DAEPLF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14098
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.078"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            95..1978
                     /locus_tag="KR314_002020"
     CDS             95..1978
                     /locus_tag="KR314_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBH0328403.1"
                     /translation="MFNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2202..2882
                     /gene="pgmB"
                     /locus_tag="KR314_002021"
     CDS             2202..2882
                     /gene="pgmB"
                     /locus_tag="KR314_002021"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBH0328404.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2879..5164
                     /locus_tag="KR314_002022"
     CDS             2879..5164
                     /locus_tag="KR314_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBH0328405.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNKVASIEKYVSITSTRDYKESELLR
                     KCEDILNIELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNCIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKYIKIDK"
     gene            5390..6283
                     /locus_tag="KR314_002023"
     CDS             5390..6283
                     /locus_tag="KR314_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBH0328406.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIAEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEV"
     gene            6331..8631
                     /locus_tag="KR314_002024"
     CDS             6331..8631
                     /locus_tag="KR314_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328407.1"
                     /translation="MKKIFSIFISCMVVVTTITGCSSGEVIKNTSPINSVKNLDFSFE
                     VKPEIFEVSVESGGSKEKISEPSKERKVSNFKKNKTEASWTYPDENIDINIKKEDSYL
                     DVNIKSNLEKETSFNWPKVEGESYILPLYEGKFIPSNDKYWKEYLNESTYNVIESFSM
                     QFFAVNKAKYSVIYIIENEFNNVIEFSSKDKINFTFKHEYPSINKNKEYGFKIYLTNK
                     NITDVAKTYKNYIVDKGEFKTLKEKSKENKNIEKLYGAPHIYFGDKSIITINDIEWNK
                     LKENLSDDLTNWMKELLSMKSGNSEFIKVINQIKTQDYIDKYQKNQIVQAFNEVMQMN
                     TFYNEKVFESKDREIKKVLDKGISKLSRVELIEFNKKLLKSKLEESVNDVATWAKYNT
                     TDVINDINKSGIKNAWIGFDDMEEGYISLEMVKRANELGYLVGPYDSYHSIHKPGEEK
                     WSTASFKDKSLYENATIENKKGEKETGFQGEGRKLNPTLSLPSVKERVQSILDEGYEF
                     NSWFIDCDATGEIYDDYSQNHITTQTQDLNARLERMSYIRDHANMVIGSEGGNDFSSS
                     TIAFAHGIETPAFAWVDKDMSKNKGSEYYVGKYYSPTGGVPEIFSKQIPVKERYKKIF
                     IDPIYSLPLFRLVYNDSVISSHQWLWGTLKIKDEVGSRMMKEVLYNTPPMYYIDRTEW
                     EKHKDEIISNNKVWSAFNKKAIKKEMTDFKFISEDRLVQMTEYGEDLKVLVNFSQKDI
                     KYNDEVIKANSLVIYDADSKVIYKPE"
     gene            8865..9356
                     /locus_tag="KR314_002025"
     CDS             8865..9356
                     /locus_tag="KR314_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBH0328408.1"
                     /translation="MYENNKVIYNFERCEKLENALYLSQFNTYKNFKLTSKKTVINSL
                     DTSTYNMNIHSISLVDTMECISSEDEQMTGKKLILVGDIDTDVTLDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            9384..10331
                     /locus_tag="KR314_002026"
     CDS             9384..10331
                     /locus_tag="KR314_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328409.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNSIVFGCIEVEIDYSCKNR
                     SNFKTVNKFNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            10621..11163
                     /locus_tag="KR314_002027"
     CDS             10621..11163
                     /locus_tag="KR314_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0328410.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            11488..12981
                     /locus_tag="KR314_002028"
     CDS             11488..12981
                     /locus_tag="KR314_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0328411.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDCNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINKGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKVNELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            13218..13982
                     /locus_tag="KR314_002029"
     CDS             13218..13982
                     /locus_tag="KR314_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0328412.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
BASE COUNT         5965 a         1239 c         2445 g         4449 t
ORIGIN      
        1 tttactatta tatattttta ttgtgatata atagtaaaat atattaacct atctttagat
       61 tatataaatt attataaagg agggaaattt taaaatgttt aataaaaata aagaaaatat
      121 aactttaagt aatcaaatca aaggaacaat tcaaaatagt ttaacaaaag aagctatatt
      181 acatatacca atgagcaatt atgcctatgg ttatgataga gaaacactac atataagact
      241 tagaactaaa aaaaatgaag cgaaaagagt aattttacga ataggagacc aatatgtgtg
      301 ggataaagga ggtgcaggag gaggaaattt aaatgcatct ggagttggtt ggtctggtgg
      361 aacaaatata gtaatgagta aagaagtaga aactgaatta tttgactatt ggatagctaa
      421 gtgtaagcct ttaaataaac gctcacgata tggttttata atagaaggtc aagaagaaaa
      481 acttttattt acagaaaaaa gaataataga gttgggaaat gaaaatgatg aaaaggaatt
      541 atgtgaaata ggtaattttt ttggatatcc atatttaaat tatatagata tacctaaagt
      601 gccaaattgg gtgaaagata ctgtttggta tcagatattt ccagatagat ttgcaaatgg
      661 tgacccgtct ataaacccag aaggagtaga taaatgggga gctattccaa ccagagataa
      721 ttttacaggt ggagatttac aaggtgtgat agaacactta gattatttga gtgatttggg
      781 aataaacgga atttactttt gccctattac aattggaaaa accaatcata gatatgatac
      841 ggtagattat atggaggtag accctacatt aggagataaa gagactttaa aaaaactaat
      901 agaagaagcg cataagagaa atataaaaat aatgttggat gctgttttta atcatatagg
      961 atattattct aaacagtggc aagatgtagt tcaaaataag gaaaattctc gatataaaaa
     1021 ctggttttat ataaaagata tgagtaaaat tgatactcca atagaacaga tagatgaaaa
     1081 aaatatacct tatgaaacct ttgggtgtga aaaatatatg cctaaactta atacagaaaa
     1141 ttcagaagta ataaaatatt tattggatgt aggcaagtac tggatacagg aatttgatat
     1201 agatgcgtgg agattagatg tttcaaatga agtagaccat gttttttgga gaaagtttag
     1261 agaagaagtc aaaaaagtaa aaccagacat ttatatattg ggagaaattt ggcatggtag
     1321 tttaccatgg ctaatgggtg accaatttga ttcagttatg aattacttga tgtcagaagc
     1381 catgaaaaaa ttcttttgta cagatgagat aaacgccgaa gaatttaagt atatgataaa
     1441 tgatgtaatg gtaagttatc caatacaagt tagtgaagtt atttttaatt tacttggaag
     1501 tcacgatact acaagaatat tgacttatgc taatggaaat attgatagat ttaaacttgc
     1561 atatatgttt atgtttgtac aaacaggatg tccttgtata tactatggtg atgaaatcgg
     1621 aatggaagga gaattaagtt caatatctga aggtcaaaga aaatgtatgg aatggaatga
     1681 agaaaaatgg aataaggata tatttgattt tatgaagaag attattagac ttcgtaaaga
     1741 aaataaagaa cttaggtcta tatcaaatga atgggtgtta gcagataagg aaaatggaac
     1801 gattatattg aaaaaagaat ctatttctat aataataaat aattcatcta aaaatataat
     1861 tttgacgtta ccagattatt tagcaaataa aaaagtaaaa gatttatatg aagaagaaat
     1921 agttgattta aaagaagaac taatgttaga gaaatacaag ttcattattt taaaataata
     1981 caaaaagtat ttattttaga tttcatgtta aattattata cagtttaaac aagtctttat
     2041 aaaacataaa aaaatatggt ttacaagtga gtgaattgaa agtagatttt aaattaaaga
     2101 ctgagttaga gtagataatt ttataaataa tatcttaaat acaagataga aaataaattg
     2161 attattagtc taaaaatttc ttgtagaaag aggaaataag aatgattgaa gcatttatat
     2221 ttgatttaga tggtgtaatt acagatactg catattatca ctatatggct tggagaaaac
     2281 tagcccataa agttgggata gatatagaca caaaatttaa tgaatcttta aaaggcataa
     2341 gtagaatgga gtcattggac aggatattag aatttggaaa taagaaatat tcattttcag
     2401 aagaagaaaa agtgagaatg gctgaagaaa aaaataatta ctatgtatca ttgatagatg
     2461 agataacttc aaatgatatt ttgccaggca tagaaagctt gttaattgat gtaaaatcta
     2521 ataatataaa gataggtcta tcttctgcaa gtaagaatgc aataaatgta ttaaatcatt
     2581 tgggtataag tgacaaattt gattttatag ctgatgcagg aaaatgtaaa aacaataaac
     2641 cacatccaga gatatttctt atgtcagcga aagggcttaa tgtaaatcca caaaattgta
     2701 tagggataga ggatgcaagt gcaggtatag acgctataaa ctcagctaat atgttttctg
     2761 ttggtgttgg aaattatgaa aacttaaaaa aagcaaattt agttgtagat tcaacaaatc
     2821 aattaaagtt tgaatatata caagaaaagt ataatgagta tatagttagg agaattatat
     2881 gaaatgttta tttagtaatg tagatgagtg gaagattata caagataaga ttgaaatcaa
     2941 agaaaataga ttagcagagt ccataatgag tataggcaat ggatatatgg gtatgagagg
     3001 aaactatgag gaaaattata gtggagatac acacaagggg tcttatatag ctggagtatg
     3061 gtttccagat aaaactcgtg ttggttggtg gaaaaatggg tatccagaat actttggtaa
     3121 aattcctaat tcagtcaact atataggtgt aagaattttc atagatgatg tagaattaga
     3181 tttagcaaaa tgtaaagtag aagattttta tagagaatta gatatgaaaa atggtatttt
     3241 aaatcgtagg tttattgtaa atataaatga taaaaaattt gaagttaggg ttactaggtt
     3301 tttaagtata tctgttaagg aacttgcagt aattaaatat agtataaaat cattaaattc
     3361 taatactaaa ataaaatttg taccatattt agattcaaat ataaaaaatg aagattcaaa
     3421 ctatgaggaa aagttttgga atggaatttc ttcaagttct ttagaatata aaggtagtat
     3481 tctttctcga acaaaggaga atgcatttaa tacaccagta ttcactgtgg gttcaatgat
     3541 gtatattaag acaaatggtg atgtagaagc tgtatctcat gagagtgatt ataattactg
     3601 tgaaaattcg ataacaatgg atgttttaaa aaataaggtt gcaagtatag aaaaatatgt
     3661 atctataact tcaactagag attataagga aagtgagcta ttaagaaaat gtgaagacat
     3721 tttaaatata gaactatcta aaagttatga tgatatatta aatgaacata caaagttatg
     3781 gaataaaaga tgggaaagtg cagatgtaaa aataaatgga gataaaagta gccaacaagg
     3841 tataagatat aatttattcc aactattttc aacatattat ggagaagatt cgcgattaaa
     3901 tataggtcca aaaggattta ctggtgaaaa gtatggagga gctacttatt gggatacaga
     3961 agcattttgt gttccagtat atctaggtgt agcagataag gaggtaacta gaaatctttt
     4021 agaatatagg tacaatcagt tagaacaggc aaaggataat gcaaaaaaac tagggcttaa
     4081 aggtgcttta tatccaatgg taacatttga tggagtagaa tgtcataatg aatgggagat
     4141 aacatttgag gaaatccacc gaaatggaag tatagtatat gcaatatata actatacaaa
     4201 ttatacaggt gactataaat atataaaaga aaaaggaata gatgtaataa tagaaatagc
     4261 tagattttgg gcaagtagag ttcatttgtc tacaaaaaaa gatttgtata tgatacatgg
     4321 agtaacaggt ccaaacgagt atgaaaacaa tatcaataat aattggtata ctaattgtat
     4381 agctaaatgg tgtttagatt atgctgtaga aaatatctta cgattagaga aagagtgtaa
     4441 tgaatgtatt gacagaaata atgtgactag ggaagaagtt gagttttgga aaataatatc
     4501 aaataaaatg tatctgccgt atgatgagga actacaaata ataattcaaa atgataattt
     4561 tttagataaa gaatttgtag aagtaaagga tttaccaaaa gaaaattttc ctcttaatca
     4621 aaattggtct tgggataaga ttcttaggtc gtgttttata aaacaagcag atgtattaca
     4681 gggaatttac tattttggaa atagatttac taaagaagaa aaaaagagaa attttgactt
     4741 ctatgaaaaa tatactgtcc atgaatcttc tttatcttct agtatttatt ctattattgc
     4801 ctcagaaata gataacctag aaaaggctta tgaattatac tcaagaacag ctaggcttga
     4861 tttagataat tacaataatg atacaaatga tggactgcac ataacttcaa tgtctggttc
     4921 atggctttcc attgtacatg gatttgcagg catgagaaca tggaatgaaa ctctatcttt
     4981 tgaccctaag ttgccaaaag agtggaaaaa ttattctttc aatataaatt atagagataa
     5041 tattattaat gtagatgtat gtagtgattt aattaaaata gaaaatttaa aaggtaaacc
     5101 aataaaaatt attgtatatg ggaaaaaata tatattagaa aaatatataa aaattgataa
     5161 gtagatttaa taatattttt attatataaa tagttaaaat aagtttattt aattatttat
     5221 ctattttaac tattttgtgt gatagaaaac tatatattat aacaatagta gtgcctttaa
     5281 ttaaatatat ttcataaatc ttcataaaat cttcaaaatt tcgtaaagaa tatgtcacaa
     5341 aagtaaggta tattatattc agcagatagt ataagggagg acaatattta tgaagaataa
     5401 aaaattcatt gttataatac tgataatatc aattttaata ggtgtattag tgaaggaata
     5461 ttcatctaga gagataaaaa aggatgatat aaatgtcagt aagtatataa aatatgctga
     5521 tttagcaagt aaaaataatg cacaagttaa ctggaagtat gtagcttcca ttgttgctgt
     5581 gttaaataaa aataacttaa aaaacgttaa ggattcacag atacaagaag tatcagattt
     5641 atttgttaag aatttttcta aaaataataa aataaataag ttaagtgata ttttggatga
     5701 gttagaattt agtaatagac aaaaaagatt ggttgataat tatatagaca atcttaagga
     5761 ttatggtata aaaccagaaa gattgaagtc tgatacaaaa tatatgaagt ttattgctga
     5821 aattaaaaca gaagcaatac aaaattataa ggattataaa atacttccat ctataactat
     5881 tgcacaagct ataatagaat ctagctgggg caagtcaact ttggcaaaac aatataacaa
     5941 tctatttggt ataaaggctg acgcttattg gaaaggtaag tcagtgactt tagaaacgaa
     6001 agaacattta gatacaatta tagatgataa atttagaata tatgatgata aaaatgaatc
     6061 tataaaagac catgcaaaat ttttagccac aaataaaagg tataaaaaca atggtgtatt
     6121 tgatgcaaaa acgtatatat atcaagctaa agcattagaa aaggcaggtt atagtactgc
     6181 gaaggatgaa aatggcaata gtatatatgc agctcgtcta atagaattaa ttcaacagta
     6241 taatcttcaa ttaatagaca gcgaaattca aagtgaagtg taacttttat gtaacatagg
     6301 agtgatatat ttatcgagga ggtggttttt atgaaaaaaa tattctcgat atttataagt
     6361 tgtatggttg ttgtaactac tattactgga tgtagtagtg gtgaggtgat aaaaaataca
     6421 tcacctatca atagtgttaa aaatttggat tttagttttg aggtgaaacc tgaaatattt
     6481 gaggtatcag ttgaatctgg tggttcaaaa gaaaagatat ctgaaccctc aaaagaaagg
     6541 aaagtatcaa attttaaaaa gaataagaca gaggcatcgt ggacgtatcc tgatgaaaat
     6601 atagacataa atataaaaaa agaggacagc tatttagatg taaatattaa atctaattta
     6661 gaaaaagaaa ctagttttaa ttggcccaaa gttgaagggg aaagttatat actaccactt
     6721 tatgagggta agtttatacc aagtaatgat aagtattgga aggagtatct aaatgagtca
     6781 acttataatg ttatagaatc tttttcaatg caattctttg cagtaaacaa ggcaaagtat
     6841 tctgtgattt atattatcga aaatgaattt aacaatgtaa ttgaatttag ttctaaagac
     6901 aagattaatt ttacatttaa acatgaatat ccaagtatca ataaaaataa ggagtatggg
     6961 tttaaaatat atttgactaa taagaatatt acagatgtag caaaaactta taaaaattat
     7021 atagttgata aaggagaatt taaaacatta aaagaaaaat ctaaggaaaa taaaaatatt
     7081 gaaaaacttt atggagcacc acatatttat tttggagata agtctatcat cacaataaat
     7141 gatattgaat ggaataaatt aaaagaaaat ttatcagatg atttgactaa ttggatgaaa
     7201 gagcttttaa gtatgaagtc tggcaatagt gaatttataa aagtaattaa tcaaataaaa
     7261 actcaagatt atatagataa atatcaaaaa aatcaaatag tacaagcatt taatgaagtt
     7321 atgcagatga atacttttta taatgaaaaa gtttttgaaa gcaaagatag agaaataaaa
     7381 aaagtattag ataaaggtat atctaaatta agtagggtag aacttataga gtttaataag
     7441 aaattattga aatcaaaatt ggaagagagc gtgaatgatg ttgctacatg ggcaaaatat
     7501 aatacaactg atgtaattaa tgatattaat aagtctggta ttaaaaatgc gtggatagga
     7561 tttgatgata tggaggaagg ttatatatcc ttagagatgg ttaaaagagc taatgagtta
     7621 ggttatctcg tagggccata tgattcatac cactctatac acaaaccagg ggaagaaaag
     7681 tggagtacag catcatttaa agataaatct ctatatgaaa atgctaccat agaaaataaa
     7741 aaaggtgaaa aagaaactgg atttcaagga gaaggaagaa aactaaatcc tacattgtct
     7801 ttaccaagtg taaaagagag agtacaaagt atattggatg aaggatatga atttaattct
     7861 tggtttatag attgtgatgc tacaggagaa atctatgatg attattctca aaatcatata
     7921 acaacacaaa cacaagattt aaatgctaga cttgaaagaa tgtcatatat aagagaccat
     7981 gcaaatatgg tcataggttc agaaggtgga aatgattttt caagtagtac tatagcattt
     8041 gcacatggta ttgagacacc agcatttgct tgggtagata aagatatgag taagaataaa
     8101 ggcagtgaat attatgttgg taaatactat tctccaacag gaggagtacc agaaatattt
     8161 tcaaagcaga tacctgtaaa agaaaggtac aaaaaaatat ttatagaccc aatttactct
     8221 ctacctttat ttagattagt ttataatgat tctgtgatat cttcccatca gtggttatgg
     8281 ggaactttga agattaagga tgaagttgga agtagaatga tgaaagaagt attatataat
     8341 acaccaccta tgtattatat agatagaact gaatgggaaa aacataaaga tgaaataatc
     8401 tcaaacaata aagtatggtc agcgtttaat aagaaagcta ttaaaaaaga aatgacagac
     8461 tttaaattta tttctgagga tagactagtt caaatgactg aatatggaga agatttaaag
     8521 gtattagtta atttttctca aaaagacatt aagtacaatg atgaagtcat aaaagcaaat
     8581 tctttggtaa tatatgatgc tgatagtaaa gtcatatata aaccggaata atagatttaa
     8641 tgaaaataaa gattgtttaa atttcaagta aaaaataata taatattcat ttagtttgta
     8701 ttaaagtagt gactatatta atctggataa ttagtatagt cactattatt tatattcttt
     8761 cctaaaagta agtagtaact gaatcgtgaa taatcaaaaa tggtaatttg aaatagtttt
     8821 tatgaatagt attaatatat agatataggg agattgatgt gattatgtat gaaaataata
     8881 aggtaatata taattttgaa agatgtgaaa agctagaaaa tgctctttat ttatctcaat
     8941 ttaatacata taaaaacttt aagttaactt ctaaaaaaac tgtaataaac tcattagata
     9001 catctactta taatatgaat attcatagca taagtctcgt tgatactatg gaatgtatat
     9061 cgtcagaaga tgaacaaatg acaggaaaga agttgatttt agttggcgat atagatactg
     9121 atgtaacatt agattatgca ggcaataaaa gaaatagaaa ttctagtaaa aaaaatacat
     9181 ttaaattaaa aatacccttt agtacattta tacaaatgcc tagaaagata gaaaataaag
     9241 ataaaattaa tttaaagtat cttatacaag atattacttc atctatttta gatgataatt
     9301 tatttattag tgttactgca attataagtt atgaaaatag ttccaagata gagtaatctt
     9361 taaattatag aaaggtggat gcaatgagaa taacgaaaac tacttttaat gatgtatttt
     9421 caaatgatgc aaatggaagt attataaaag tagaaaaata tgttgataaa aaatgtgcat
     9481 ctgtatttga gattttgacc tacactatta ttgttacaaa tacaagtagg tacaaaactg
     9541 gaaatatatt tttcaaagat tatatttcta aatatataga gtttataaat aatactgtaa
     9601 aagttaatgg cattataaaa cgtggattag acccacagca aggtttttac atagggagaa
     9661 tagatgcaag ttgtaaaaaa ataatatctt ttaagagtgt agttttacca aattctgcac
     9721 ataggattat aaagaatagt gctaatatat actattatta taagtgtaat ctagataaat
     9781 tcccaacaag aatatcaata gaaagtaata gagtttatac taatgtaaat aaaattgtat
     9841 tcaaacaatt gaatataagt aatactttaa aagctcctaa aaatctaaga gatatattaa
     9901 aagtaaatgt aaatagtaaa atatttgatg ttaaacctat gaaaaatctt ataaacaaag
     9961 gtagtaattt gaaattatgt aactcgatag tttttgggtg tatagaagtt gaaatagact
    10021 atagttgtaa aaataggagt aattttaaaa cagtaaataa gtttaatgat attaaaaaag
    10081 ataagtatta taaaaatgat atacaaaata acaatgaaaa tataaacgat tgtaaaaagc
    10141 aaataagttt tggtgataat aaggtaaaaa aaataacaaa aacttttgga ttttcttgtt
    10201 tcatttgttc tccagttgga attatttatg aagatatgaa aaatataaat ataaaaattg
    10261 aacatacatc aataaatgaa ttaaatccag aagaattatt tgtaaataca tcacttttat
    10321 tgtattatta agtattttaa agctaaaact taaacttatt aaaatagcat ttggttaaag
    10381 gaaaatagat attgatatat ttataatata tataaatgat taattaaaca attataaata
    10441 ataagtatag tataatattt aaaaaatata gacttatgat aaaataagac aaatatgtta
    10501 aaaaaatgat aatcacggag aacttttacc ttcttcaatt aatatattat tattagtaat
    10561 aactttaaaa ttatattact attatataaa aattggctta aaagaaggtg aaaaaagtaa
    10621 atgaaaatat gcataacagt aggtcatagt atactaaaag gtggtaaaag tacaggtgta
    10681 aatggtatag ttgatgaata caattataat aaaaaattag ctcctatgtt agctgagatg
    10741 cttataagtc aaggtaatac agtagatgta ataatttgtc cagaaaggca ctttatgtca
    10801 gaaagagaag aatttttcta tagagttcca aaagtaaaca gtggtaaata cgatttatta
    10861 gtagagctac atttaaataa agctgatgga acacaatgtg gtacagaagt gctttactat
    10921 ggcactgaag gattagaata tgctaaaaga gtatctaata gacttggaga attatttgaa
    10981 aatagaggag ctaaaaaaag agaaaattta tatatattga gaaatacaaa tccagttgca
    11041 atacagattg aaagtttttt ctgtgataat gtgaatgatt gtaacaaagc aaatgaatca
    11101 ggttatgatt atatagcacg attgattaca gaaggtatat tgaataaaga tataaatatg
    11161 tgagattatg gtgatttagg tattgattaa taccttttaa tattagatat aaataaaaat
    11221 acaatgtatt agtataattt aaactttcaa aatatagatt agtttgatta ttgacaagag
    11281 atacctccta aatattgatt tatcagtata tttaaggagg tatttaaata taaaaattta
    11341 attgatttgc tactaaacta taggtttata gagtatatat ttttatcttc agtctaataa
    11401 ataatataaa aatagtagaa ccttgtatta taataatata atgtagaaaa atatatagat
    11461 aagataaata taataaggtg gtaatatatg aaatatacat ttgaaaaatt taagttaaat
    11521 gaaaagatac ttaagtcatt gaagagttta ggatataata taccttctag agtacaaaga
    11581 gaagttattc caaaattatt aaaaggtcaa aacttagtag ttaggtcaaa aacaggaagt
    11641 ggaaagacag caagttttgc aataccttta tgcgagaata taaatgtaga ttgcaataat
    11701 attcaagctc ttatagtggt tccaacaaga gaattagcct tgcaagtaaa agatgagata
    11761 tcagatatag ggagactaaa gaaggtgaga tgtagtgcta tatttggcaa acaatctata
    11821 aaagaccaaa tagctgaatt gaaacaaagg gttcatattg ttgtagctac tccaggcaga
    11881 atacttgacc atataaataa aggttctatt aagttggaaa atgtaaagta tttagtaata
    11941 gatgaggcag ataaaatgtt taataaaggt tttgttgaac aaatggaaaa aatattatta
    12001 aatttaccta aggagaaaat agtaagtctt ttttcagcta caatagatga agagataaaa
    12061 tatatatgtg aaaaatatat gctagattac agtgttataa atatagaaga aaatgagagc
    12121 gatactaatc aaaaaacaag acaaatagat gacaaaataa tcaaggcaaa tggaagagag
    12181 aagtacatcc ttttaaaaga attaatatac agcgaaaatc caaagagtgt gataatattt
    12241 tgtaatacca aagagaaagt atctaaatta tataataaga tgagtaaaga gggtttttta
    12301 ataagagagt tacatgcaga tttatcacaa gaaagaagaa tttttgtaat taaggatttt
    12361 aaaaaccaaa aatttaatat attagttagt agtgatgtag cgtccagagg aatacatatt
    12421 gatgatattt ctttagtaat aaattatgat gtgccacaag ataaagaaaa ctatatacat
    12481 agaataggta gaactgggag aaaaggaaat agtggaaagg caattactat agtaactgaa
    12541 aaggatgaaa aatatataga gaatatagaa acgtatatag gatataaagt aaatgaatta
    12601 aaagatatag aaaaaagtag aattacacat ggcaaaattt tgtttgaaga atattcaaaa
    12661 ggaatcctaa aaaaagtatc aaagagaaaa aaagttgata aaaattctta caaaagtaaa
    12721 gatataaaac taaatataga aagtgaagtt gttaaattat atttaaatgc tggtaagaag
    12781 aagaaaataa gagttttaga tatagttggt gcatttagta atataaaagg tataactaat
    12841 gatgatatag gtgtaattga agttcaagat ttatgttcat atgtagatat attaaattat
    12901 aagggagatt tgatattaaa aaaatataag gagataccta taaagaaaaa aatggttaaa
    12961 gtgaaaagag atatacgata atttagttat tactgctctc tctgatgatg aaatcatata
    13021 tttaagtaag agtatcaata tttatgaatt attctgttat cgaaatgaag tttcaaaaaa
    13081 caatagaagt tgttaaggaa aggaatgttg actttatcta cattctaagc tatactattt
    13141 ttaaatgttt atgacaatag tcattttaaa tataagtaat aaatatatac ctatagggat
    13201 attaaaggag gtaatatatg gcatatttta actataacga caaacagtgc tactataatg
    13261 aaattgggga tggtacacca ttactttttt tacatggaaa tacagcctct tccaaaatgt
    13321 ttaatgatat aatcgatttt tataaagatg aatataaagt aatattaatt gattttgtag
    13381 gtcatggaaa atctcaaatg gtcgataaat tttctgctga cttatggttt gatgaggcta
    13441 tgcaagtgat atgtttttta gaagcaatga actataaaaa agtaaacata attggtagta
    13501 gtggaggagc tcttgttgca ttaaatgttg cacttgagag acctgattta gtgaataagg
    13561 taattgcaga tagttttgaa ggagaagttc ctttagagtc atttgtacaa aatgttaaaa
    13621 tagagcgtga ggcatcaaag caagatgatg gtgcaaaagc tttttatatt tataatcaag
    13681 gagaaaactg ggaaaaagtt gtagataatg atacagaagc tatttttgaa cattataaaa
    13741 ctataggaaa attttttcat aagcctctag aaactatgca accagaggtg cttttaacag
    13801 gaagtagaga agatgagttt gtatctctaa tatctagtga tttttttgaa aatacttttt
    13861 cattacttct tgaaaagatt aaaaatggta aaatgtattt gtttgataaa ggtggtcatc
    13921 ctgcaatttt atcgaatgga ttagattttt caaatgtagc aaagaaattt ttagaggaat
    13981 aatttaacca aaatttacaa taatgaaaac tgtaaaatgg attgcaaaac ttacaaaagt
    14041 gagtgtacat atacttcatt attatgatga aataggattg ttaaaacaaa atattcta
//
LOCUS       DAEPLF010000079        14021 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEPLF010000079 DAEPLF010000000
VERSION     DAEPLF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.079"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(204..1637)
                     /locus_tag="KR314_002030"
     CDS             complement(204..1637)
                     /locus_tag="KR314_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBH0328413.1"
                     /translation="MVLRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSREVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMTKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(1724..3640)
                     /locus_tag="KR314_002031"
     CDS             complement(1724..3640)
                     /locus_tag="KR314_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0328414.1"
                     /translation="MRRYEQLSKEIIENVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASIGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVIIIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGVLTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTSVVAAMYFKLKDKKIKELCIPAVISGICGVIEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDSIVEISKDNNGQGVVEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTIMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKHFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIIENTQ
                     KHVNNSQELITVLA"
     gene            complement(3714..4574)
                     /locus_tag="KR314_002032"
     CDS             complement(3714..4574)
                     /locus_tag="KR314_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0328415.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKCIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(4925..6112)
                     /locus_tag="KR314_002033"
     CDS             complement(4925..6112)
                     /locus_tag="KR314_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0328416.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGDNKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIESGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(6126..7517)
                     /locus_tag="KR314_002034"
     CDS             complement(6126..7517)
                     /locus_tag="KR314_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBH0328417.1"
                     /translation="MSSLKKKKFTMPQTLTIIFSLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMVFLTIGLYLGF"
     gene            complement(8112..9812)
                     /locus_tag="KR314_002035"
     CDS             complement(8112..9812)
                     /locus_tag="KR314_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0328418.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLASNGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(9784..11187)
                     /locus_tag="KR314_002036"
     CDS             complement(9784..11187)
                     /locus_tag="KR314_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0328419.1"
                     /translation="MIVNKELIIDYLKSNQENIIKDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGENGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTDDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSSAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(11451..11942)
                     /locus_tag="KR314_002037"
     CDS             complement(11451..11942)
                     /locus_tag="KR314_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0328420.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPNEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(11968..12780)
                     /locus_tag="KR314_002038"
     CDS             complement(11968..12780)
                     /locus_tag="KR314_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0328421.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLMGYEYKKVLSKEAIKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(12793..13944)
                     /locus_tag="KR314_002039"
     CDS             complement(12793..13944)
                     /locus_tag="KR314_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH0328422.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
BASE COUNT         4359 a         2718 c         1470 g         5474 t
ORIGIN      
        1 gacttagcac ttatagctta taagtcttta tattattaat ctatttttat aggttaattg
       61 tacaaatcat atatcacttt tacctattaa atgcattata aaaaagatgg aataacgatt
      121 cgtcactcca ccttttttgt aataaaaaat tgtatatata aacactattt actactcttc
      181 taagtacaaa cagtatctac atactataac ctttcaccat tagattctat tacttgttta
      241 taccagtcaa aactcttttt cttaatccta gacaagtctc cacttccatc atcatactta
      301 tccacgtata taaacccata tctcttggtc atctcaccag tagatgcact tactaaatct
      361 atacatcccc atggagtata tccaaataaa tcaacaccat cttctacagc ttccttcatt
      421 tgctctatat gtgcttttaa atactctata cgatagtcat catttatcac tccattagat
      481 tcttttttat cataagctcc taaaccattt tcaactacca tcataggtat tctatatcta
      541 tcataaattt catttagtgc atatctaagc ccttcagggt ctatttgcca tccccattca
      601 ctagacttaa gatatggatt tttcactcca cctaatatgt tgccacttgt attatcttct
      661 ttagaatcac tacttacaca tgtagacata taataactaa atgtataaaa atctacacaa
      721 ccttctttta aaatttctaa atctcctttt tccattttta tttcaatatt attttctcca
      781 aaatatctat tcatataagt tggatattcg ccacgcactt gtacatctcc acagaaataa
      841 ttcattactc tactatctct ttgagtttta actgcatcat ctggattaca agtataagga
      901 tacattgttg caaatatttg catacatcca acttgataat ttgaatctat ttcatgagct
      961 aatttaacag ccttagcact tgctacaaat tgatgatgta acccttgaaa acgaagttga
     1021 ggtatatcta cttggtttat aaaatctgta gtcccttcat ttaatattcc ttgtgataag
     1081 tagcctccca taggcattgt agctgaattt atttcattaa atgtcagcca atattttact
     1141 tttcctttat atcttttaaa tattgtttca cagtaattca tatacaagtc tattacctct
     1201 cttgaaaccc atccattgta tttctctgtt attgcaaatg gtatttcata gtgagatatt
     1261 gttattaatg gttctatatt atattttaaa cattcatcta tcacattttc ataaaattta
     1321 agtcctgctt catttggttc tttctccatt ccagttggaa atattctagt ccatgctatt
     1381 gaaaatctaa ataccttaaa tcccatttct gcaaataaag ctatatcttc tttgtatctg
     1441 tgataaaaat ctattgcttc atgacttgga tagaatgttc ctttttctat aactctcgtt
     1501 attcttttac ttactttttg agaaccccca gtacacatat ctgaacaaga aggacctttc
     1561 ccatctaaat tccatgctcc ttcacattga tttgctgctg ttgctccacc ccataaaaaa
     1621 tcttttctaa gtaccattac tctacctcca ataatttatt tatagtaaat ttattttaaa
     1681 ataaaaatac atatatctaa tttttaatta ataaaactct cttttaagct aaaacagtta
     1741 tcaattcctg actattattt acatgctttt gtgtattttc tataatatcc acataatcag
     1801 aataattagt aacaactact ggtgtttcta agcaatatcc ttcttttgat atgctttcta
     1861 tatcaaaagt tattaatgtc tgaccttttt taatcttatc cccttgatta acaaaagatt
     1921 tgaaatgttt accttccaac tgaatagtat ttaaccctat atgtatcaat agttcacaac
     1981 cattatcaga tataattcct attgcatgtt ttgttggaaa taaagtcatt atagtcccat
     2041 caaatggtgc cactactttt ccttctttag gtgttattgc tacacctttt cccaatatac
     2101 ctgttgaaaa cgctgcatct tgtatctgct ccaaagcctt tacttcacct tttattgggc
     2161 tatatactac ttctcttttg tttttttcta caacgccttg cccattatta tctttagaaa
     2221 tttctactat agaatcatct ttccaaaaaa ataatgttaa aacaaatcct actacaatag
     2281 ctactattat tgctattata gttgcataaa cacctttcat atctccagtt gttggattta
     2341 tataattagg tataccaaat attccaagtc cacccattac ataacttttt acattcatta
     2401 tccccattac agctcctgcc acagcaccac ctacacaact atatatgaat ggtgtttttc
     2461 ttggtaaagt tattccatat attgcaggct ctattacacc acatatccct gaaataactg
     2521 caggtataca cagctctttt atcttcttat cttttaattt aaagtacatt gctgctacta
     2581 ctgatgtttg tgcaaaagat ggtacaaatg ctcccactaa tattgtgtca aaccccatag
     2641 tcataaggtt tgacatagct attggtacta atccccaatg tagaccaaat attacaagca
     2701 cttgccacaa taaaccagtt aatacaccac ataaaattgg tgaaaagtta aataaagcta
     2761 aaaatccatt tccaactata ttagaagcat aggtagctat aggccctata accataaatc
     2821 ccaatggtaa agaaactaat aaaactgcca ttggtacaaa gaaattcttt actacatctg
     2881 gtattatttt agcaaacact ttctccaact tagctgctaa taatactatt ataattactg
     2941 gtactactgt tgaagtgtag ttcattgcta ttactggtaa tcctaagaaa gttatatata
     3001 ctggtgattc aaatattgtt ccagtaaata gattgtataa aggttgacct atagatgcta
     3061 gagtatctaa ttgaatacct gaatagcata aaactgcacc tataagcatt ccaataaatg
     3121 gttttaattt gaatttctta gcagctgtat atccaagaat tattggcata tactggaaca
     3181 ttgcatctcc gattgcattt aacataatat atgcaccatc agtttctcca tacaaaccca
     3241 atgctgtaaa tactgcatta aatcctttta tcataccagc agcacacaga actcctaata
     3301 ttggttgaaa aactcctgat attatgtcta taaacttatt aagtaatcct tttggcgcat
     3361 ctgaatctga attatctatt gtatctcctc ctaatccagc caaatcgcac acaacttcat
     3421 aaacatctgg aacatgatta ccaataacta cttggtattg acctgcactt ttcattacag
     3481 taactatacc atccattttt tttagcacat catcatttgc tattgattca tcttttaatt
     3541 taaaacgtag cctcgttata cagtgagata aactgctaat attatccttc cctcctacat
     3601 tctcaatgat ttcttttgat agttgttcat atcttctcat tttttctcct ccattttctt
     3661 tttgaaatat gaaggtttgg ccaacttaat gtcaccaccc aatatatata aacctaatta
     3721 ttaggattta tatctgactt aatactttta cttaccactc tttcaacgtg aatagttaag
     3781 tataattttt cctcatctgt taaatcgtag ttgtattttt tatgtataaa ttcatccaac
     3841 ttttctatgc atttataagc atttttatat ttaactttta ttacatttag taaatcatct
     3901 tcattatttt catgtaaatt attactaaca attcgttctg caaaatactt taagtgagta
     3961 ataaatctat gataataaac tgaatcttca tcaaatacta ttctgtaatg gtatttaacc
     4021 atatttgaaa tttcttgtat tatttgtgta atctcatata tagtttttat actttgatcc
     4081 atttgtgcat taactatatg cagtgctata aatccagctt catcttctga tagtctaatt
     4141 tcaaattttt tttctatata atccaatgct tctaacccta ttttaaattc actcttataa
     4201 aatcttttta tatcccaaag taatacattt tttacactta ctccttcacg agctctttct
     4261 attgcagtat acatatggtc tgttaaagaa atataaatac tatcatttaa tcttttccct
     4321 agtttttctt tagcatataa aattatttca tctgataatt ccatatatct tataggaata
     4381 tctgctatta gttctttaaa tttatcagat atatttgggt ctgaaagttt gaatatctta
     4441 tcaattttat cctttgatat atgctctcct gtacgttttt tataagctaa acctctcccc
     4501 ataactacta tttccttatg attttcatca gttgtaataa ttacattatt attgagtatt
     4561 ttttcaataa tcattgtgat gcccccttta ttagcattaa gttaaaataa atctttttta
     4621 attatttaga atatttaaaa tgtaatttat tacataaaaa aaaacctatt tattacacat
     4681 taaatagact cccatgagtc ctgagtaata aataggttta gcttgtattt aaacaatcac
     4741 tatccgtttg tattttattt taattattat ttattatatg ttagcaaatt tattttttag
     4801 tgtcaacttt ttctcaatat tttaattcta aataaattat ataaattgca tctgcaaagt
     4861 actattttta taaataaaca aaataaaaaa ggtattactt gctttaagta atacccattt
     4921 atttttaatt taagtggttt ttcttaatct catcaagtag tccatcttct aaataaattt
     4981 ctaatgcaca tcctaccatt gcctttactg ctttgtgaag tttatcataa gcttcctcag
     5041 aatttgcata atttagaaat tctttttcat gaacatatac cattggactt atatctaagg
     5101 ctatttctgt atacatagta gggcaaactt gacttacatt acctatatct gtagaaccta
     5161 ctggaccatc tttaccttct aatatatctg ttatacctga ctcaatcaaa ttattctttg
     5221 ttatcttttg aagtactttg ttatttatta tgttgtcaaa agaattttca aagtatctat
     5281 aagaaagctt agctcctgtc attaactctg caccttttgc acagtttata acttttttag
     5341 tcacttcatc taaataactt ctttcagcag ctcttacaaa aaacttacaa gctgcttttt
     5401 ctggaactat atttggagcc tctcctccat ttgaaactat tccatgtatt ctaacatctg
     5461 attttacatg ttgtcttagt gcatttatcc cagcaaaagt aagttgtact gcatctagtg
     5521 cattaactcc catatgaggg tgactagctg catgtgctga taccccttca aaactaaact
     5581 ctaatgaatc tattgccaaa gccttacagt ttatattatt aaaagcttct atatgcattt
     5641 gataacttac atcaacatca tcaaaagctc ctgccttaac taaatcacat tttcctcctg
     5701 ttgtttcttc agctggtgtt cctattagta caatttttcc tttaaaattt tcttttaact
     5761 tacttaatac tatacaagct cctagtgtag atgctgcaat ccagttatgc ccacaagcat
     5821 gagctggttc tttattgtct ccatatccag gaagtgcatc atactcagct aaaaatgcta
     5881 tggtaggtcc atcattatct cctaattcag ctctaaaagc agtctccata ttacaatatg
     5941 gataaactgt atcaaatcca tattccttca ttttctctac taagtatttt gatgatatgt
     6001 attcttcttc ccctaattct ggattgttaa atataaaatt acaaacttct tcaatttctt
     6061 ttccaagttc attctctatc tcataagctc tcttctttat actctctatc ataacttacc
     6121 tcccactaga atcctagata taatcctatt gtcaaaaata ccattacaag aattgttaat
     6181 actaccatta aaggaattac gaatctaacc cactttgacc aaggaatatt ggctactgct
     6241 agtgctccca ttaaaacacc tgatgtaggt gttattaagt tagttacacc tataccaaat
     6301 tgataagctg taactatcat ttgtcttgat acatctacta agtcgccaag tggtgcaagt
     6361 acaggtattg taagtgatgc aagccctgat gctgatggta ttaaaaatgc tattaatcct
     6421 tctattaaga aagttaaatt tataaatata gtttttggta atccatttaa taaatttgct
     6481 gcagaattta aaattgtatc tattataaat ccattttctg ctattattac gattccacgt
     6541 gcaaatgcta tacataaccc tgctgtaatt aaatctcctg ctccacttat aaatgaatta
     6601 actatctcat cttgtttaag ccctgatgct attcctgata ttatacctat agctgtaaat
     6661 atcattgcaa tttcagttat gtaccactct agtcttaaaa ctccatatat cataatcgcc
     6721 atacctattg tgaaaatggc tattaccatg gcttctcttt ttgtaaattc ctttatattt
     6781 gtatcactat taaccattga ttcttgattt agtgatatat cgtgtactaa agatttttta
     6841 gggtcttttt taactttatt ggcatacatc ataacaaatc ctatagatat agccatatat
     6901 attataaaca taacaactct aaactctatt ccagaacctg gtactatttg tgataatgct
     6961 tgtgctattc ctacagagaa aggattcgta gtagctgctc caaatccagc tgttgctcct
     7021 ataaatactg tggccactgc tgtcaatgaa tcatatccca tactggtcat aagtggaata
     7081 aatatcatat aaaaaggtag tgtctcttca ctcattccaa acgtcgtccc acctagtcca
     7141 aatagaatca ttgaaatcgg tattattaaa aattgaaact tcttcatttt atttacagta
     7201 tgagctataa cagcttctat agcccctgtt ttattaacta tcccaaaagc tcctccaact
     7261 attaaaacaa atccaactac ttcagctgca tctataaatc cgtttatagg tgctgtaaat
     7321 acatcagtaa ttccttgtgg atttgatggt gctttttcat aagttccagc tacaacaact
     7381 gaccttccgt ctatatctac tctttcaaaa tttccacttg gcactatcca agtaagaatt
     7441 gccattaaca ctattagcga aaatattatt gtaagtgtct gtggcatagt aaactttttc
     7501 ttttttaaac tgctcatttt taatctcccc actttttaaa ttaaatattt tattattgca
     7561 tccctttatc ctattttgta tagatgtaaa aaagctcaac atggatacat gttgagctaa
     7621 atgtgctaaa tacaacatac ctgcatcttc cctttatctt gtttaagata gctctccatc
     7681 aaaattcggg ataattttga tgacagtact gtacctatta aatacagtcc cagcactagc
     7741 tttggacact agcacttcgg cacttactcc ttttatattg cctcattggt ttccaacctc
     7801 ttcaatatta atcttagtaa atgcaacctc tacctcaatc tgttcgttag atgaggtaat
     7861 tcttattaat ttttattgat tcaaagtaat tgtagcataa tatgtcgaga ttgtaaatat
     7921 tttcagtata ataattctta ctttatgatg tcattccatt tttcacaaat aattttttct
     7981 ccatataatt taaatactgt agtatatttt aaatacacac aaactttcca caaattttta
     8041 attattataa tacctttata ataaaaaata aaagccatgt aaacttatca atttacacag
     8101 ctttatatac attaatctac tccaagtaca gctttaagct ttgatgctat tgtaataact
     8161 cgtggaccaa atataacttg aactgagttt ttctttttaa caactccact tgcacccaat
     8221 tcatttaccc agaaaccatc ttcatagaat ccatcaattg aatttagctc taccctaagt
     8281 ctagttgcac agtttgtaac attttttata ttattacttc ctcctaaacc ttctattata
     8341 gaatgtacaa gctttgtatc tttatcatct acttcctctt ctactgcatt gttgtatgct
     8401 gttttaccag catctacaga ttgtctttct ctatattctt tcttactata aagcttaact
     8461 tcttctgaac ttcttcctgg tgtttttata tcaaacttct taattgccca tgtaaatatt
     8521 atataagtaa ctataaaaaa tattggtatc aaaaataata atggtaacgc atccactttt
     8581 tgtggttgta ataaattagg aaacatattt ctaaatgatt ctcccattat agaaacatta
     8641 aacatttctg taaatacata cgtcaatcct gccagtggta catatgctac aaaatacaat
     8701 aatggtgcaa caaataggaa tgtatactct attggttccg ttacccctac caacatggct
     8761 gttactattg ctggtatcaa tatacttatt gcttttttct tattagcatt atcagccaac
     8821 ttatacatag ctaaagctgc tgctggtaat attgcaaaat gtattaataa acgacctgtt
     8881 gtgaagttac gagttatata agatagtgta tctgggtctc ccatttgagc taatcttata
     8941 ttattaacac cctcgtaaat tgctccacct acttccattg ttccacctac tcttgtatat
     9001 tctataggga atgcaattaa gtgatgtata ccaaatggta ataatgcttt atccattgct
     9061 ccaaaagtaa atgttcctaa taatccacta gaacctatcc aattaccaag tttagataat
     9121 gccaacccta ctggtggcca tataaagtac attgctgtac ccaatactat tgatgctata
     9181 aacatcataa tcattgaaaa tcttggacct gagaaaaaag ataatgcact aggaagttct
     9241 atgtcaacaa atttgttatg tactgcacta gctacaaatc cagctattat agctccaaat
     9301 atactacaat catatgaaaa gattcctaag aaagaaccat ataaagaact tcgactatat
     9361 gcttctgcct ctcctaatcc agatgccata aacgcttctg ctgtaactgt atctgaattt
     9421 ataccatttg atgctaacaa tgtacttata actgtgtgca ttcctataaa taaaacaatt
     9481 cctcctaatg caccccatcc tttttccttt ttagataatc caaaagctat acctgtaaca
     9541 aagaatattg gtaagtatct cattaccata aatcctaaat cgtttattac tttaaagaat
     9601 acatttataa atgacccttc ttttattagt ccgctaagcc ctaatgcaga aacgttttcc
     9661 atatttgcaa aagctgaccc taatcctata aatactcctg ctacaacaag taatataaca
     9721 ggtatcatca ttgctccaga aagtacttgt aatttatcct tcatagtatt atttcctcca
     9781 tttttattga aatgccaggc tatataatgc atatatatat acttctattg ctttgtataa
     9841 tgtatctata cttatatttt catttgcctg atgtgaattt ggattatctc cctctattgt
     9901 gggtccaaat gcaactccat tttttaacac tcttgcataa gatgcagctc cttttgtaaa
     9961 aagttcggct tcttttccaa aaccttgttt atatgcactt gataatttat ttattaattc
    10021 actatctggt tttacataca gtaattttaa atccttatat atatcaacta ttaaattttc
    10081 ttttttccca atttcattaa ttctattagt tataaactcc caacttatac ccttaggata
    10141 tctaaagtct attttcatat tcaattcatt tttctctaag aagatactca ttatacatat
    10201 tgtagaaact cccatatcta catcttcttt atatatccct aatttttttc catgattatc
    10261 atctgtaaag tatgaattta aaatatccct tattataacg cctttatcat ttagcttttt
    10321 aattttctct aaatcttttg ctaaactaaa tgcacagtta tatgacctgt gtggatttct
    10381 cgataaagcc ctttctcctg tatatcttac taatacttta ccttcttcta gttcttctat
    10441 ctctgtataa taagctaatg attctactaa atctttctta tcatcagttt taaaaacagc
    10501 ctcaattttg tcacatacaa atccatcttc tttgtttgac tttatatcaa ctaaattgtg
    10561 atttctaaat ttatctttat ctattttctt tcttagattg aagtataaaa taccattttc
    10621 tccatttact attgggaagt ttccatctgg agaaaatgca aattttggct gttcattgac
    10681 actaaagtaa tgctccatac attcccaagt cgtctcttca gctccaccca ctatcaatct
    10741 aatctttctc ttcggctttt cattaagttc tcttaagaaa agtaatgcat ataaaatccc
    10801 tatcaaaggt cctttgttat cattaactcc tctaccataa agatagttat ctttttgaca
    10861 tactttaaaa ggtttactct tccaaagttc tttattatat attggcacca catctatatg
    10921 attaagcacc cctactactt cttctccttc tccatattct atatgaattg catatccatc
    10981 aaagtctttt acaacaaaat ctttgtcttt tgctatttga ataagcttat cgaaggcatt
    11041 tcttatttct atcccaaatg gttgatttat atctgttgta ctttcatctc taattgaagg
    11101 tatttcaact aaacttctaa tatcctttat gatattttct tgattggatt ttaaataatc
    11161 aataattagt tctttgttta ctatcatctt aattatttat caccccttta tattaatttt
    11221 acaaagcatt tcagttcata tctacattat aaattttgaa tattattttt tcaatatttc
    11281 aaatacttct ctaaattacc ataatgatac ggtaaacaat caacagatta tgacaaaatc
    11341 atactatacc tattactaat attttaactt atctattacg tcttttttaa tgtaatttta
    11401 ttccaaaaat agatataaaa agatgtagcc ttagctacat ctttttatat ctattcaact
    11461 tccattatta tgctattaat atctacattt ccaggttcta tcctatcttt tactgttctc
    11521 ttaacagaat cagcatcatc cattataact acaggagtta ttatatttaa atcatttgac
    11581 gttataaaat ctaaatctac ttccattatt aaatctttag cttttacctt atctcctgcc
    11641 tttaccttaa catcaaacgg ctttccttct agtgacattg tctctaaacc tacatgtatt
    11701 aatatatttg tattatcatt actcttaatc attattgcat gctttgtatc aaatacattg
    11761 actatttcac catcaacagg agaatataac ttaccttcat ttggtattat tgctactcca
    11821 tctcccatca tcttttgggc aaaaactgca tctggaacat cttctaagtt cactaaattt
    11881 cccttaaaag ggctatatat tttctcaatc ttattttctt ttttcttaaa tatattaaac
    11941 ataacacact ctccatatta taaaatttta tgataatatt tttcttaaag cttttgctac
    12001 accatcattt acattagtat cagttacaaa atctgccata gattttactt catctgtagc
    12061 atttcccata gcaataccaa gacctgcaaa tttaatcatg gatatatcat tttcattatc
    12121 acctatacaa ataacttctt tgctatctat acctaatata tcacaaaatt ttttaactgc
    12181 aatacctttt gaagtacctt tactcataac ttcaaaattt atctttccag aacttactac
    12241 ttctaaatct tcttgttttt taaattcttc ctttgcttct ctcaaagaat ctacgttttc
    12301 tgagaaaagt atacatttta aaatttcatt atcagctttt tctaataaag tttcacaact
    12361 tgcattttct attatttcaa ttctgtcttc ttctttattt ttactattta ttaatctata
    12421 tggatgttct tcacctattt cattgttgga tataactatt ttacagcctt taaaatgttt
    12481 gtttaaatta tacttttcac ctattgtata gattttcttt atagcttctt tgctcaaaac
    12541 cttcttatac tcatatccca ttaatttaaa atatgtccca ttagttgata taatatatat
    12601 atcatcccct aaaacacttg taaaatattt catagcatta taaggtcttc ccgtaactaa
    12661 ggctaccttg ataccctttt ccatagcttc ttttagtgct ttaatatttt cttctgatac
    12721 ttcaaatcct ttccccatca aagtaccatc catatcagta caaattaatt tatatcgcat
    12781 ttgtatcaca tcctagtaag atttttctaa tatttcaaca aggtcttctt tctttccaaa
    12841 aggtaaaaat cctaaaactg gacagcttgt tatttgttct aattgttcct ttgttacatt
    12901 aaactcagac atttttgtct taagtcccac tctctttaaa aaactttcta tttcatttcc
    12961 taaagtttta gctgcttcat catcttccac tttttctaat tcttggttta gcattctcgc
    13021 tactttggca aatttctcta tattatcctt atagaacttt tctgcaaatt ttgggaacac
    13081 tatagccaaa gcctcaccat gaggaatatg tgtaactcct cctattattt cacttagtgg
    13141 atgtggtgct gctgctccag catttgccag tgaacttcct cctaatgtat ctgctactgc
    13201 taactttgtt ctgtattcta tattattttt atcttccatt actttaggta gatagttaat
    13261 gactaattcc atagattttt cagacattat ctctgtaaag tcattagcat ttggatttat
    13321 ataactttca aatgcatgag taaaagcatc aaatgctgtt gatgcagtaa ttctctctgg
    13381 cagagttaac ataagctctg ggtcaactat acattctttt gagaaacaat tttgatgaaa
    13441 tattgtattt ttctcgcttc ctctcgatat tactgctgct tgtgtaactt ggcttcctgt
    13501 tccagaagta gttgatacag ctattaatgg taattctttt tcacttacct tatcgtatat
    13561 tttaaatgga tttgtatatg tactaaacat ataatcccaa tctatttttt ctaaaccaaa
    13621 agtcaatgct aatacttttg cagtatctat acttgaacct cctccaacag ctaaaacaac
    13681 atctacagat tcttttttag ctaattcgaa cccttcattt attaattcta cagttgggtt
    13741 tggctctact ctatcaaagt gtacaacttc tatacctttt tctattaaat tcaatgtaac
    13801 tcttttatat aacttgtcta atggctctac atttggagta gtaactaaca aacatttctt
    13861 tccatatttt cctattattc taggtagttc acttaaagtt ccttgtccaa aatttattct
    13921 tgatggttga taaaagttaa tcatattttt cctcccttaa attattgact tcatattcaa
    13981 attatacata tcataggatt tttaaggcaa taccttcact g
//
LOCUS       DAEPLF010000080        13913 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEPLF010000080 DAEPLF010000000
VERSION     DAEPLF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13913
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.080"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(84..245)
                     /locus_tag="KR314_002040"
     CDS             complement(84..245)
                     /locus_tag="KR314_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328423.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(586..1026)
                     /locus_tag="KR314_002041"
     CDS             complement(586..1026)
                     /locus_tag="KR314_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH0328424.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(1098..1925)
                     /locus_tag="KR314_002042"
     CDS             complement(1098..1925)
                     /locus_tag="KR314_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328425.1"
                     /translation="MEFESGIKLGKLTLIKYLGRNKHSKKVWLCQCECGNKVERVENN
                     LKQSIKNEKPPHCGCSPNWKGQNKRFKDITGKTFGKLTVLKMSGKDKYSHNLWLCQCE
                     CGNRTITSTSALEQGKAQSCGCIRKEILLERSTTHNKSNDSLYRVYHRMIKRCTNKSN
                     KDYNYYGGRGIEVCNEWLEDFINFYNWSIENGYRKGLTIDRINVNGNYEPSNCRWVTW
                     EVQRNNKRNSIRVNYKGEMITLKQCAENLNVKYLFLYNQLVTKKIPLEEVIKNIGME"
     gene            complement(1984..2454)
                     /locus_tag="KR314_002043"
     CDS             complement(1984..2454)
                     /locus_tag="KR314_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBH0328426.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVESFALLNGML"
     gene            complement(2471..3781)
                     /locus_tag="KR314_002044"
     CDS             complement(2471..3781)
                     /locus_tag="KR314_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBH0328427.1"
                     /translation="MALGGGTFVTQNKILPGAYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEAFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVILLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSIENKIKDKDLVESSLIYWATGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAIKVINAMSKLYMTVSVN"
     gene            complement(3782..3958)
                     /locus_tag="KR314_002045"
     CDS             complement(3782..3958)
                     /locus_tag="KR314_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328428.1"
                     /translation="MSKALSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            complement(3951..4388)
                     /locus_tag="KR314_002046"
     CDS             complement(3951..4388)
                     /locus_tag="KR314_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328429.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEDKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKNNDTNKMDTVELFGGLKRGDNFE"
     gene            complement(4737..5543)
                     /locus_tag="KR314_002047"
     CDS             complement(4737..5543)
                     /locus_tag="KR314_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328430.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNKLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            complement(5653..5943)
                     /locus_tag="KR314_002048"
     CDS             complement(5653..5943)
                     /locus_tag="KR314_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBH0328431.1"
                     /translation="MARWGSVDFREFKRVCKKMEKFTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLREGWSGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            complement(5943..6290)
                     /locus_tag="KR314_002049"
     CDS             complement(5943..6290)
                     /locus_tag="KR314_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328432.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(6284..6685)
                     /locus_tag="KR314_002050"
     CDS             complement(6284..6685)
                     /locus_tag="KR314_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328433.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            complement(6695..6928)
                     /locus_tag="KR314_002051"
     CDS             complement(6695..6928)
                     /locus_tag="KR314_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328434.1"
                     /translation="MPQVKKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLSV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(6947..7837)
                     /locus_tag="KR314_002052"
     CDS             complement(6947..7837)
                     /locus_tag="KR314_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBH0328435.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATENNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTANVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEA"
     gene            complement(7849..8412)
                     /locus_tag="KR314_002053"
     CDS             complement(7849..8412)
                     /locus_tag="KR314_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBH0328436.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTVTQEDYVNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDMEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(8480..8728)
                     /locus_tag="KR314_002054"
     CDS             complement(8480..8728)
                     /locus_tag="KR314_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328437.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            complement(8730..10265)
                     /locus_tag="KR314_002055"
     CDS             complement(8730..10265)
                     /locus_tag="KR314_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBH0328438.1"
                     /translation="MKHKDYWRKRFEQLEEVQNNKSVKYYLELEKKYKLAMNSIEKDI
                     LSWYNRFAENEGISLLEAKKLLNTRELEEFKWNVEEYIKYGKENAINQKWMKELENAS
                     ARLHITRLEALKLQIQQHVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRPTLINELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLDVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     NGE"
     gene            complement(10255..11697)
                     /locus_tag="KR314_002056"
     CDS             complement(10255..11697)
                     /locus_tag="KR314_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH0328439.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEMQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKSVQITFNHSMIINESEKIDMASKSIGIISDETIVSNHPWVEDVNDE
                     LERLKKQEETQKEYDDLIPTNNQEGVIDET"
     gene            complement(11703..12956)
                     /locus_tag="KR314_002057"
     CDS             complement(11703..12956)
                     /locus_tag="KR314_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBH0328440.1"
                     /translation="MVIEMIVRINFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANAIL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSRGLEKTLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            complement(12934..13626)
                     /locus_tag="KR314_002058"
     CDS             complement(12934..13626)
                     /locus_tag="KR314_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0328441.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWSSE
                     KKKGAPINKRGAPFSNKNSVGYGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHEITENSEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVANLNKDDIGDDEL
                     KISVDYGDRNDS"
BASE COUNT         4055 a         2671 c         1423 g         5764 t
ORIGIN      
        1 tctaatttgt ggtataataa aagcaagaag aactacaatc tatttgacag tagagtgaag
       61 ttcataattt aaacaattag taattattta aatttgtgga acttgatttt aaaatcaaat
      121 tcccagccac ttttactctt gccacgagtt gagtggcttt ttacttttag aaatacttta
      181 caaattaagc aaaatattaa actagcaata acgccagcta ttacattaag taaaaagtta
      241 ttcatacttc ccacctcctt tcattaggaa gtaggtttta tcccagtatg aactccactc
      301 tataaattgt agattacatc ttcttgctaa aaatattata acatataatt attacatatt
      361 ttacctattc tatatttatt tttttatttt gctatcttct tcgtcccctc tttctctctc
      421 tttcagcttc tttcattgct tcctcttcat cttctatctt tataagtatt gaggcggctg
      481 ctaacgctct ctcattaact tctaaattca tatattcact tggcttccac tttaattttt
      541 gaatacaata atgagtgatg ctagcatcaa aatcgccacc tctgattagt tttttgcttc
      601 ttctacttta tcctcaaaag atgtatcaaa tccattgact tcattcactt ttactgtata
      661 attgacatac tcacctgctg taagcattgt ctttaataac tgagcttctc ccattactcc
      721 ataactattt tggagttcgg catcctttaa atctggaaat actgtagatg ctacacataa
      781 ttcagctaca taactattgt agtcaatttc acttgtatat tgtccagtat gcttaccatt
      841 gttaccaatc acttttactc ttttagtaca ctttcttctt agtgcttcgt cttcttcaga
      901 tgataaaact tttaattccc attcaactgg tttcccttct ttatctaaaa atctgttact
      961 cgctacatat tttacattat caacctttat tgcattttga cttaaaaaag cacttaaatt
     1021 actcatatta ttctaatctc cttttatttt aatttttcat ataaaaaata cacatttata
     1081 acttataaat gtgtatttta ctccatacct atatttttta ttacttcttc caatggaatt
     1141 tttttagtaa ctaattgatt ataaagaaat agatacttaa cattcaaatt ttctgcacat
     1201 tgttttaaag taatcatttc acctttataa ttaactctaa tactatttct tttattattt
     1261 cgttgaactt cccaagtaac ccatctacaa tttgatggtt cgtaatttcc attcacatta
     1321 attctatcta ttgttaaacc ttttctatat ccattttcta tagaccagtt ataaaaattt
     1381 ataaaatcct ctagccattc attgcatact tctatgcctc tacctccata ataattataa
     1441 tctttattac tcttatttgt gcacctttta atcatacggt gataaactct atataagcta
     1501 tcattagatt tattatgcgt tgtagagcgc tctagtaata tttcctttct tatacagcca
     1561 caactttgtg cttttccttg ttctaatgca cttgtactag ttattgttct atttccacat
     1621 tcacattgac ataaccataa attgtgacta tatttatctt tcccagacat tttcaatact
     1681 gtaagcttcc caaaagtttt cccagttata tctttaaatc tcttattttg tcctttccag
     1741 tttggagaac aaccacaatg tggaggcttc tcatttttaa ttgattgctt taaattattt
     1801 tctactctct caactttatt cccacactca cattgacata gccagacttt tttactatgt
     1861 ttatttctcc ctaaatattt aattaaagta agtttgccta atttaattcc actttcaaat
     1921 tccatgaaat acctccaaat aatttatact tacatcataa tatttttcaa gtataattca
     1981 attttataac attccattaa gtaatgcaaa ggattcaact aattcccaat cctcaaaagt
     2041 gaaatccata tcttcatcta aatactcacc atcagcatca aatttagtaa ttattccact
     2101 gtccatatta caatctttaa gtactactgt ctgtcttcct acagcagatg tagggtcttc
     2161 atttgtaact tgtatgtcaa aataaatatc ctcaccagtt tctttatatc tgtaaagtaa
     2221 ttctctaaaa atagaagtat tataatgaaa tgttgcactt ccagtatttg tactcccagt
     2281 tgttttattt ccctttgttg ttcttcctag aattggaact tcacttttat ttttttccat
     2341 tttagcctct aaatctatag cttgcataaa attatatctt ttaccttcta tagttataaa
     2401 acattcagct ttctttgcac taactgtatc tttagcattt attgtttgag ccatattatc
     2461 actctcctct ctaattaact gaaacagtca tataaagctt actcatagca tttattacct
     2521 taatagcatc agatactatg acagttttct tatcatttcc aagctctaca ctaacatcat
     2581 cagttttaaa atcttctatt gcccttatat tctctaattc tttatggtgt ttaacaacat
     2641 cattccagaa acttattctt cctgccttat cattcggaac tttacctaaa tacttttcat
     2701 taaataaagt tgcaatatca ttagcaattt ggtcaagtac tctaacactt tggttacttg
     2761 aaaaatcgtc atttttatca tctgtaaatg atacaaaagt atttatatcc tctaacacat
     2821 gaacttcatc tcccaccttg tggaatataa atttaccagt ttttaaagct tcttcaagtt
     2881 gtatttgtgt ataattaaca tcaacatcaa actcaccatc atacttttta ttagtattag
     2941 atttatttat atcgcatcct gctatagctc cagtagccca ataaatcaag ctagattcaa
     3001 ctaaatcttt atctttaatc ttattttcta tagacactac accttcataa tctgcatcac
     3061 ttttcttata tagtactgtt tgaaacttag ctcctacctt atctctcatt ctctttgtaa
     3121 attctacaaa taaactttta atttctgttg ttgtagccaa acaccctagt gcattaaatg
     3181 aataactttc tattttatcc aagaaagctt ggtactctgc tcctgtcaca gcttcgccat
     3241 tggttccacc agtaaataca agtcctgcac ttgcttctag tgttgcatcc ttcttccaag
     3301 tgatatagtc attgtcttgt aagtctgtaa taacctttgc tatttgagta tctaccttct
     3361 tattatctaa aagtattaca acatcaaact tagcattatc atctatattt gttgtaactg
     3421 ttacttttaa gtcatttcct cttataccac tatattttgc tgtggctata gtacaactgg
     3481 ctttaacgcc tttatttaat ttataaaaat atcccaacct tatatttttg aataaatctc
     3541 tcaaaccttt cagcttctca tgagtataat catatccaaa atacttcact gaatacttct
     3601 caaaatcatc actggttact tggaatgctt cttcatctat gccccaatct aactctaaag
     3661 gcattgcaac aatacctcta tctgataatg aactggttgc cctcttagct gagataaaat
     3721 ttatatatgc accaggaagt attttatttt gcgttacgaa tgttcctcca cctaaagcca
     3781 tctaactcac tcctttcata aatttattta ttctatcctc tacctctgag aaggaatata
     3841 actcattttc ttttaaaatt gcatttaata aatcttttct atttacatac ttcttagagt
     3901 taactatctg ctccttagta aacttgtagt tatcttcttt gcttaatgct ttactcaaaa
     3961 ttatcacctc tcttcaaacc accgaataac tctactgtat ccatcttatt ggtatcatta
     4021 ttttttatag taaaatagtt ataatcaaca aagaagtgaa gaacattgtc tataatttca
     4081 aaattcatat ttgtacctct tactaaatct ccatcaattt ctatatactc taattcctcc
     4141 agtagcatct cagctatctc atttatttca aaattcttat cttctgaacg agggaaataa
     4201 tgtacatcaa aagaattttt ctttaattct ctcccgcttg gatatggtgt cttgcttgga
     4261 tttaaaggaa caataaaaaa acaaggttca ttaatacctt gctccacatc ttcgctataa
     4321 attgtatatt tttctccaaa cgatttatct aactttactg atattccatc tataatatta
     4381 ttaagcatca aacactcctt taagcaatat taatagtttt ttctctatta tcttatcaac
     4441 ttgactttgt agttccattt ctgaaatagt taagaaatgt tgtcctttaa cccaaccctt
     4501 gccattttta gttctatgac caaattcaac atatgcattt tgttatccta aaggcttttt
     4561 atcctctagc tcttatagtt tcctataagt tcggcgtaca tcatcaacaa aataaacttt
     4621 atttagttgc ccagcactct tggagagatt atatttattc actctctacg ctctacggaa
     4681 acctatagcc tatccgcaat ctataggttt tcctcggtat tggcatatat aattaattaa
     4741 atatttttcc atgttcttct atggactata tttgatatag atgaatatgt tacaggatat
     4801 atttcactta gttgttttat tgtatatcca tcagaatact tttttctaat ttcttttaca
     4861 tcctcaatag ataacttagc tctgctttct tcaataatct taactttttc tataagctta
     4921 tttcctacta atcccttaga atatctactt cttaagcaag agtatgaaat acctgttttt
     4981 tcagaaagct ctatcaatgt aatttctttt tcttcatgtt ttactaatat atttgttgac
     5041 ctatttctac attgtgtctt tatatccacc catctacaat tgcttgattc ataatttcct
     5101 tcattattta ttctatcaat tgtaagtttc tctgaatatc cattactcaa tgcccaattt
     5161 gcaaaattat catagcaaaa ccattcatca cacacaatta tattcctttc accatatcta
     5221 ttgtatcttt tattattttt atctgtacat ctagatatta tacccttcca tatactgtaa
     5281 agtcttttat tttgaacctt atatttcttt ttaaattggt atttgtctgt taaatttaat
     5341 ttatcttgtt ctttttttaa acatccacaa gattgcacta agccactagt taaagagtca
     5401 gttcttattt ctttgaaatt tccacaatca cacttacata accaatattt cctatttctt
     5461 ttaccgcttt ttatctcttt agaaaattta atgactctta gccttccaaa tttttttcct
     5521 gtaatatcta aaaattttgc cataaaatca cctcttttat acattataaa tcaagagtta
     5581 ttctatgtcc atattctacc tactttataa tgtaacacta ttacacttag cgtttaccga
     5641 ttttgctggg ttttatatgc cccattgtgt taaggcatat tcagttggat taacaacttc
     5701 tataatataa ttattacctt gtttgtaaac aggaagcgac ctagcataag ctactccact
     5761 ccaaccctct ctcaaaaatc ctgtatcaac tggtgtcctt ctaattactt ttcctaagag
     5821 tcttgctgct aattctcttg ctgcatcctt gcaaaactta tctaaatcaa tctttgtaaa
     5881 tttctccatc tttttacaaa ctcttttaaa ctctctaaaa tcaacactgc cccatctagc
     5941 cattatgctt tatccttaaa taactcaagt attatttctt gatgatttgg atatatagct
     6001 gattctccac ttcttacata ctctttatca tttataataa gttttgaacc tgctttaatt
     6061 tctatatctg gagatataaa gagtttaata gtttgctcta gcttagctaa tttcccttct
     6121 gtagcagaaa ctatattttt atatgaaagt ttacatggtt gattttctaa tacaatcact
     6181 tctttattgt tagttcgttt tgttacaggg tctttaattg gctgatactc aactatagta
     6241 catttatctc tatataacat ttctattgct tttctagttt tacttaccat cttaagcacc
     6301 taaaggttaa tatcttattc ttaccataag tagtaagata agctattaag ctatcaaagc
     6361 gttgttctgg tgtttgagaa ccacttccta tagcaaaatc tacctttgta tcaccttctg
     6421 atatagactt ttctacagct tcaaagttaa tgctttctat atctaattga cccatatttt
     6481 tcttggtaaa taagaactct ccaactatca tatcaacttc aatttctttc aattcaattg
     6541 gcatagtttt tatattacaa tctagtttaa taatattttc tattttttct cttacaaaac
     6601 ctattaacca cttatctcca tcttttaata tatatccaaa actttcaagt cttttttcta
     6661 tttcatcaat tatattattt tccataattt tcacctactt tttagtaagt ttatttttct
     6721 ctttaagctg cttattttct tcttctaaag actcaacttt tgaccttaaa atattatttt
     6781 cagctattaa atcttttaca cttaatgact tgccatactt tactgcctta ccagtttcat
     6841 ctatcaaatc atatcccatc tctaagaaat catctatttt acattcttct atggttagta
     6901 ttctatttag tttttttact tgtggcacta tatatcactc ctttttctat gcttcaacaa
     6961 caaattgtat tgcatcagct tttttattta atataaatac atcctcaaaa ctttcttcaa
     7021 agtagaagta ttttccctct gtaactgctg ttggttcgtc taacttagag aactgataag
     7081 aaacaggtgt aattattgca cttgggtgaa ctaaggacat aaagatttgt ttagctcctg
     7141 ctcctacttt ccatccagtt gtaaaatcat atgcagtttt cattagatta gatggtactt
     7201 taattatttt aactgtgtca atatctgtgg tttgtctatt taaagaagtt ccaccatctt
     7261 ttatgtttac tgttctttgt atctcttttg catttttgat aagtgtattt actactggag
     7321 taacatacaa tattcttcca ttttcaggta ctctagcttc tgtcattttt tccattaact
     7381 tatcaaatac ttctaatacg tttgctgttg taagaacagt tgtatctgct gtgttaccta
     7441 atgcggtcca atcagcatat attttagata tacagtaagc atccatctct ggaaactttt
     7501 gttcctcatt atatactttt gttatattac ctattgaagc cacataatta gtttggttta
     7561 tatctgctgg atgaaccaat gttgaccatt tcctttgatt agttaatacc ttaggttccc
     7621 aagcattatc atagtttctt tgagctactg ctattgtatc tctgtttgaa tctacccttc
     7681 cagttgtaga tatagttggt atttctattg ttttagaacc cgtccatcta tatcttccat
     7741 tattttctgt tgcatacaaa tccccgaagt ttaaagtata aggatatgct tgtgctaaaa
     7801 catttgaata ttcttttgca taatttagtg ctgccatttt atttcctcct atttattatt
     7861 attttcatga ggtcttaccc cagtaaaatt aaaaccaaaa tcatttatct taggctcttg
     7921 ccctggtgtt atagtatcta ttttaggttc ttcaccttct agtgttgcat taaacaaata
     7981 atctttatcc tgtttcaaag ggtttatttg ctcttcaaaa gctttttgtc tatctttact
     8041 atttcttagg gcttccatgt ctaaatgagc ttttaacgct atttcatctc tacatttaac
     8101 agatttaaaa gcatcaccta accagtaatt aaaatctttt tcttcaattt cttttttgta
     8161 ggtttcttcc aaagttttct tatcagtttc ataagttgtt tttagattct ctacatcttc
     8221 ttttgtcata cctccttcaa actttttaat agtttcatta gctgtattaa gttgtgtttc
     8281 aagatttaca taatcttctt gagtaactgt agtctccttt attttctttt ctatagattt
     8341 ttgaagagaa gctacatcaa ttttgttatc ctctactttt attccttcta gcaattcttt
     8401 taaccaatcc attttaaatt tctcctttca ttttttacaa aataaaagca cctactaatt
     8461 tattatttag caagtgcttt tacatattta ctatttgtat atctttccat aaatccttta
     8521 gtaatttacc atcaatgttg taattatcaa ccatatcctt accattttta taatactttg
     8581 tatctccatt agggcaaaga gtaataaacc ttgtatcatc atctccaata gatatattat
     8641 atggtttatt atataagtca aattctatat ctaatcctaa atcaatagaa tcaattaaat
     8701 gttgtaaatt cttaaattta ttatccattt tattctccat tcaaaatatc tttgttagct
     8761 attttatgag cttttgtaag ctccatatcc ttctctcttt ttaccttatc atggttattt
     8821 tcatcagcta accaatcatg tttatgaggt acaattttat gttgctttgg gtttctatgg
     8881 tcagttaagt ctaaatctaa tctaggtttt cctgtattac catagtatct tctttgaatt
     8941 aatttaccat ctttgtaatt atcaaataca ctatttggtt ttgattcaaa tggcactgaa
     9001 tgaacacttc cactagttaa atttctctga ttcttaactt gccaatttac atccttataa
     9061 agctttttag cttcctcata ccttatagta tcattatact tcatatgttg atattcatca
     9121 aatttagaag gtatttcatt tcctaatacc tttttatatt cttcaaattg ttttctatct
     9181 ttactctcat ttagttgcat ttttctaagg gtgtcagctt tttcttttcc aagtctaccc
     9241 tctatatgtt tctcatacca ctcattatac ttcatactag atggtacata atatgttttt
     9301 ccatcttctc ctcttgctgc tctatagcct tcttcatcct cgaaccaagg agctgttgtt
     9361 gtcctacaac gacaatgaaa tggtggagct gttattccaa cttgataatc tttcatatca
     9421 aatatttttc catctaactc tctgcatata tttgaagttc ttaaatctaa tgtagcaata
     9481 atctcatatt tctctacatc taaatcacta aaacaatctt ttctacttgc tgatgcaaag
     9541 aaagctgatt cagtcattat caaattctta gcttgtgatt tagatacatt aaatctctta
     9601 gcaaagtcat ttactagatt ttttggattt tcacctctaa taattgattg agttagctta
     9661 gtatgtagtt catttattaa agtaggtcta tgcttacccc aaattctttc actaaagttt
     9721 aatccatctg ttgcccatgg tttagagata attttattta ttctattagt atcaagactc
     9781 attaaactcc aaccaacgtt tactccttgt tgaacattaa aagctgtatg atagtatcca
     9841 cttgtataaa tatctctcat tagtttatca ataccatcaa gttcatttcc atataaaact
     9901 tctacgtgtt gctgtatttg taactttaaa gcttcaagcc ttgttatatg aagtcttgca
     9961 ctagcatttt ctaactcctt catccacttt tgatttatag cattttcttt gccatattta
    10021 atatactctt ctacattcca cttaaattct tctaattctc ttgtatttaa cagtttctta
    10081 gcttctaata aagatattcc ttcattttca gcaaatctgt tataccatga taatatatct
    10141 ttttctatac tattcatagc taatttgtat ttcttttcca attcaagata atattttaca
    10201 cttttattgt tttgaacttc ttctaattgt tcaaatcttt tcctccaata atctttatgt
    10261 ttcatctata acaccctctt gattattagt aggaattaaa tcatcatatt ctttttgggt
    10321 ttcttcctgt tttttaagtc tttcaagttc gtcattaaca tcttcaaccc aaggatgatt
    10381 actaacaata gtttcatctg atataattcc aattgattta gaagccatat ctattttttc
    10441 agactcattt attatcatag aatgattaaa tgttatttga actgacttat aatcatagct
    10501 cttactacca cttatcttta aatactcaca cacaaaccat aaaagctctc taattgcttt
    10561 tttaaacttc ttttcagtct tggaacattt taaatcaagt agtgaatata aaaatttaag
    10621 agctacaccc gatttgtcac ctgtgttttg agattcggga ttaactcctt gaccaaaaat
    10681 aattatattc ttttctaatc tatcaagaag ctcctttttg gcttcaactg gtatatttat
    10741 ctctagttta tcaactccac ctccaccatc tactttaatt gatttatagt atcttatatt
    10801 atctataaac tcttgtagac ttgttcctgg atactctttt aatacataaa tgacctcttg
    10861 catttcatct aaattgtctg caagtgtaga aatgttatta tcatatatat caattaaaga
    10921 cttatagaaa gttaaatctg agacacactt ttcattattt ttaaaaggta taaatggaac
    10981 tttaccccat ccctgttctt tgttatttat tctaaaatga ccttcttgta tatcagtcat
    11041 ttttccatat tcatcatata aaaattcttg aataaaacta ttacctcttt caataaagta
    11101 agttacgtca ttttctgtgt agtactcaac tctttttatt ttatttccat ctatatcttc
    11161 aatataataa aacctaataa atgcaactaa ttccctctgt cttttactat cccaaatagg
    11221 aattgcttct tcagctggaa ttattacata tttaaactca ccttttctat taatatatgg
    11281 atgtaaccat tcaacccctt tattactagc attgagatat agttctgtta ttgtatcgtc
    11341 aaactcttct cctagtaagt catttaaaag cttagtgaga ttatcatcat ctgcattaaa
    11401 tactatggga tttccgacac tatagcctac cttttggtca actaaaagct tatggtagtt
    11461 gttaattgct ttattattaa ctttagtaaa atcatcaacc ttagctccat ctaagagata
    11521 atatcttctc ttattgttta catcagtatt accataatag tattcttctc cttgtttata
    11581 tttttctggc ctatgcttta agatgtagtg ttctatgact tttactaggt taaaggtgct
    11641 ctcttttttt aactgaactt ttattaaatc tgtttcactt atataaatat ttaacacctc
    11701 ctttacttta agaagcttat tccattattt ttaagcttat tatctataga atatctaaga
    11761 gcagccattg catcatccat aaactcaact ggttcatcaa gatataatcc agttctttcg
    11821 tcttgtttcc atttccattg ttgtatttct tttatggtat tagtgcaact aggatgtaca
    11881 tgtattctta attgtttcaa ataatctatt tgagctttaa cacttcctgg tcctttttta
    11941 actcctttag ctttatatcc tgcactcttc cacatcttaa ttctatctgg ttcagcacta
    12001 tcacagtaca taaatagagt cttttctaaa cctctactat ttgcaatttt tatgatttct
    12061 gaggtatcca tttcatgtac atatatttca ttacatatat ataactctcc atccttaaag
    12121 ccaattctaa gtattgcatt tgcatgatta aagccaaagt cttgtgacag tctcatattg
    12181 tcaaagtatt caaattctgt gggaaattca tgtataacat aatttttaag tattgctcca
    12241 ccagtttctc cccattctcc aagaccatat actttgtacc cttctgggtc ttgctctttt
    12301 ctcatttgca ttcttctgta gtaagcttca tctatgaatc tattttgtag ataagtacta
    12361 tgatgagtaa atatatcatc atttttatag tcaaaatact ttctttttat ccaatgagta
    12421 gctgagactg gattaaatgt aaatgtcatt tgataataca agttaggatt agttaaaata
    12481 cctcttaaac ggtcatctag tatatctatg tcactttcca taagttctgt agcttcttca
    12541 caccaaaccc atgttaattt tcctttcgag aagttaattg attttaattt ttctctttgt
    12601 tttgcatcat taactcctct gaaaattata gagttaccag taactttact cttaatttct
    12661 aaaggattta aagtagtttt ccaatactta tcagcttgtt taccataaat acgatttata
    12721 gctcctgtaa gctctgcata cgttgaatac ttatgtgtag cttctgactt tctaactact
    12781 aatagattag ctccttgata cttcttatct cctaacttta gtatatagtc ttgtgctaca
    12841 ttaacagatt ttccactccc tgctgaacct ttcattgctc tgtatctttt tttagtaaaa
    12901 ttagcttcct tgaaatctgg attaaaattt attctaacta tcatttctat caccataatc
    12961 tacacttatt ttcaactcat catctccaat atcatcttta tttaggttag caacctcaca
    13021 tttcaacttc tcaactcttg ttttctgctc ctctgtagcc aaattccaat ccttatgaat
    13081 catttcatca tactgcttaa ttaaactcct taactcactc atagctctac tctgtgcatt
    13141 aagaaaagat gcttgcctat cccatgcaaa ttgaaattca tactctatct tctcactatt
    13201 ttctgtgatt tcatgtttct ttaattcctt aatcatttcc tctttatctt taacatacat
    13261 tatcttctgt gctcttatta ttgctgcgta ttgaattgtt atctgttccc aaagaatatc
    13321 aaatttatct tttatagata tttcttgtat taattcccta gtttcttcag gtaagtattt
    13381 tgagaagaaa ccaaactttt cagcattttt attctctttt ggagcaccat aaccaactga
    13441 atttttatta gaaaagggtg cacccctttt atttataggt gcacccttct ttttttcact
    13501 agaccaattg tatcttttta tccatgactt taaagtattt aaactaatgt catactttgc
    13561 tgatatttcc ttttgtttca ttccttttaa gtaatcttgt tttacctttt ctttgacatc
    13621 ttgcacatca ccacctcttt atttgtttgt tttgggaata aaaaaacaat cctatttaat
    13681 aggattgttt caaatctagt ctcctctatc cttccaggat aaatgaaaca tacttattat
    13741 taatgtagtt agagataaaa ttatatgaac tgtaaaaaat attaaaacat agaaaattat
    13801 attagaataa tatcgagtaa gcattattgt attgataaaa catattacaa caaccaaaat
    13861 ctccattatt acagtataat atgcttttaa ctgtatgaat tgaaagtatg ata
//
LOCUS       DAEPLF010000081        13841 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEPLF010000081 DAEPLF010000000
VERSION     DAEPLF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13841
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.081"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..477
                     /locus_tag="KR314_002059"
     CDS             <1..477
                     /locus_tag="KR314_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBH0328442.1"
                     /translation="PDAFSKYKLKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSR
                     LSQELGVICHFLCDFFCVAHSERWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTIN
                     SSFEKFFTKLYSDYKKKTDYMNDLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(581..1360)
                     /locus_tag="KR314_002060"
     CDS             complement(581..1360)
                     /locus_tag="KR314_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0328443.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(1430..2686)
                     /locus_tag="KR314_002061"
     CDS             complement(1430..2686)
                     /locus_tag="KR314_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBH0328444.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            2944..3843
                     /locus_tag="KR314_002062"
     CDS             2944..3843
                     /locus_tag="KR314_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBH0328445.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            3867..5315
                     /gene="ddlR"
                     /locus_tag="KR314_002063"
     CDS             3867..5315
                     /gene="ddlR"
                     /locus_tag="KR314_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBH0328446.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            5374..6180
                     /locus_tag="KR314_002064"
     CDS             5374..6180
                     /locus_tag="KR314_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0328447.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKISTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            6206..8125
                     /locus_tag="KR314_002065"
     CDS             6206..8125
                     /locus_tag="KR314_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0328448.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVKRGAQ
                     ARSTKQKARLQRFDELMNRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(8367..8714)
                     /locus_tag="KR314_002066"
     CDS             complement(8367..8714)
                     /locus_tag="KR314_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBH0328449.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(8701..10629)
                     /locus_tag="KR314_002067"
     CDS             complement(8701..10629)
                     /locus_tag="KR314_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBH0328450.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTTLPIVLSKVKNNEQE"
     gene            complement(11040..13229)
                     /locus_tag="KR314_002068"
     CDS             complement(11040..13229)
                     /locus_tag="KR314_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBH0328451.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEYNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKKALPE"
     gene            13560..>13841
                     /locus_tag="KR314_002069"
     CDS             13560..>13841
                     /locus_tag="KR314_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBH0328452.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLYMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQNNKECKIPTLEEV"
BASE COUNT         5036 a         2115 c         1786 g         4904 t
ORIGIN      
        1 cctgatgctt tttctaaata taaattaaaa aaacattatt tagatgagtc atttaatatg
       61 attgtttcta aaataaacta tctatgcagt cttaatatag atagtctttc taaatacttt
      121 tcagtaagta gacttagtca ggaactagga gtcatatgtc attttttatg tgattttttc
      181 tgtgtagctc acagtgaacg ttgggaattt aagcatagct taaataaaca tgttacttat
      241 gaaagagaac ttggagcatt tgcaaaagat gttgatttat ctaaaattaa atgtggtact
      301 ataaatagta gttttgagaa gttttttact aaactttata gtgattacaa aaagaaaact
      361 gattatatga atgatttaaa attttcagct tatacttgta atagtgtcat aaattatata
      421 cttgactcta ttttatctaa tacaataaaa tcatatcata tagcaaattg cggttaactt
      481 aagaattttt aaatttatat aaacttataa ttgaatataa ttaaaatgac tgctctatag
      541 aatataattt tatacttctc taggacagtc attttttata ctaactattt atctccatta
      601 taacatctat ggcacttttt acactatcta ctacataatt tgcactttgc tttacatctt
      661 cccttgcctt agacattgca aaactatatt taaatccttc taacatgcaa atatcatttg
      721 gagaatcccc aatagtagca acttcatctg gacttacatt taacgttttt attacatgct
      781 ctatacttcc tctttttgac acacttttag gaactatatt taaagtatat gttccagaaa
      841 aatatatttc taatacttca ctaaactcac tttttagata aagttctatt ctggataact
      901 cttccaaatt ttcagaacat acgcctatat ttcctataag attatcaaaa ctacttcttt
      961 tttcaaaatc tgggtcaact ataaagtcac tagaataatg tctaaaagca gtggtatcac
     1021 tacccacaat tacatcaccc ttatatttaa aataaagttg tgcatcactt gattcaaatc
     1081 tatcaatgac tttttttact aaattatcat ctatcgttct tttaaaaatt acattataat
     1141 ctttatcata tataaatgaa ccattttcac atacataata tccattcata tctaaactat
     1201 ctttgataat attatgtgct tgtttaaaaa ttctacctgt agcaacatta aactgtactc
     1261 cctttttttc tatttcttca attgctacta tatcttcttt tgttatggtt ccattttcat
     1321 atagagttcc atctaaatca caaaatatat gttttatcat aaattaaaag cctccaaaaa
     1381 caattttata ttatagtaac acttcaaata tatccaatat cataacaact taaagctttt
     1441 ctaattgtat tctcaattca ttaagtaaat ctctagtagc tcttaatctt atctttcttc
     1501 tatccccatt aaatatattt ttctttacta ttgtttttcc atttatatat attgcagtat
     1561 aaactagtcc cacaggcttc tctttggttc ctccaccagg tccagctact ccagttgtgg
     1621 atatacctat attagcttta agtcctttag caacgccctc agccatttct atagctgttt
     1681 gctcacttac tgctccaaac tcttccaaag tttctctttt tactcctaat cttttcattt
     1741 tagaatcatt agaatatgtt acacatcctt caagaaatac agaagatatt cctggatagt
     1801 ttataagaga tgaagaaacc aaacctccag tacaagattc agccactgct attgttagat
     1861 ttttttctac aagtaactta gaaacagcat cttctaaagc agtctcccct tcagatatgg
     1921 aaatatctcc ttcagtatat acatatttac caactctact ttttattttt tcttctactg
     1981 gttttattaa tttaaaagct tctctttcat tttcagcctt tgcagttatt cttattgtaa
     2041 cttctaattc ttttgcatat aaagcaactg ttggattagt ctgctcatta attatatcga
     2101 gaatttctaa ttctagattt gactcaccta ttccatataa tcttaaagtc ttagatacta
     2161 gcatttcatt tgtgaattgt tgtaagtaag gtttgacact ctcattaaac atagctttca
     2221 tttctcttgg aggtccaggc aatactatta taaatttacc atcttttttt aaaattgccc
     2281 ctggagctgt accattatta ttttttagta ctatagcatc ttttggaaag tacacttgtt
     2341 tcatattatt ttcggccatt ttttttcctg ttttaacaaa gaaagattcc aaaacctcta
     2401 gtgaaggttt atgtagttct aatttctgac caaagtattc tgcagcagtt tcttttgtca
     2461 tatcatcacc agttggccca agaccacctg tagtaattac aaaatcactt ctctttaagc
     2521 tttcatcaaa acattctaaa agtctttgtt tgttatctcc aacagtactc tgatggtata
     2581 cttctattcc caatgatgca agttctttag ctaaaaattg agaatttgta tttactatat
     2641 ctcccaataa tatttctgtt cctacactta ttatttcagc tttcaaaatt tatcactcct
     2701 tgatttatag ccattaagac tagtcttaag ttcataacaa taattttagc aaaaataata
     2761 acataaataa agttaaaaaa tcatttaaac atatctttaa actagattat tattctaaaa
     2821 ccacacataa tataaacttc gtatatagtg gtactttcat cctcaactgt taattcatat
     2881 aattaagtat gtaaataaaa agtttacttt aaaataataa aaattaaaat aggagtgtat
     2941 aatatgaaaa tagcagttat aatgggtgga atatcatcag agagagaagt ttctttaaat
     3001 tcaggaaaag agatatataa taaccttgat aaaaataaat atgaagttgt aaaagtaata
     3061 atagatgata aaaaagatat atttacaaag ataccagaag atatagattt tgcaatactt
     3121 gctttacatg gaaaatttgg tgaagatgga tgtattcaat caatacttga gactatggat
     3181 ataccatact ctggatgtgg acctctttgc agtgggatgt gtatggataa aaatataact
     3241 aaaaaaatgc ttagagattc aaacctacct actgctccat gggttttagt aaaatcagtt
     3301 gatgaaatcg actatgatga aatagataat ataggttatc cagtatttat aaaaccaaac
     3361 tcaggaggtt caagtgtagc cacattcttt attcattcta aggatgaagt tgaagaagca
     3421 gttagaaaag gtctggaagt tgacgaattt gtaatgattg aaaaatatat accaggtgga
     3481 gaatatactt cattcatatt aaatggagaa gtgttcccta caatatctat aaaatctgat
     3541 tcaggattct ttgattatga agctaaatac tcagttgaaa aaggtgcaaa ggaagaagtt
     3601 gtttacttag atgaagaact tcaaaaaaga gtaaacgaaa tttctgagac ttgttggaaa
     3661 atttttaatt gcaaagcata tgtaagagtt gatatgatta taagtgaagg tattccatat
     3721 gttcttgaac taaatactct tcctggaatg actcaaacta gtttaattcc tagaagtgct
     3781 gctgctagag gtattaagta cagtgaacta ttagataaac ttatagaata ctctttaaat
     3841 tagatgaaat aatttgaggt ggtaatttga tattttcaaa tctaaaacta aatgataatg
     3901 aaccaattta tatacaactt aaaaattata taagtgatat gatatcaaaa ggactaattc
     3961 cagacaatag taagctccct tctacaaggg agcttagtca gcttttacaa gttagccgaa
     4021 attctgtagt tttaacttat gaagaattaa aatcagaagg attaatctac tctatttcag
     4081 gaaaaggtac atttgttaaa tcaaagaata agtcttcaaa tactacatgg tcattaaact
     4141 gggattgttt agagaataca tactctaaaa aagccaatga attggacatt ataaaaagtg
     4201 aaattccttg gagctctgac ctgatttcct ttaaaagtat ttctccagat ggagatttat
     4261 tcgacatgga agaattaaaa aaatcctttt taaatagaat ttcactagaa ggacataaat
     4321 tactcaacta tggatatgca caaggttata agccccttat tgactattta ctagaatata
     4381 tgacaaacaa gggtgctgat ataagtaata aagatatact aataacaaat ggattcacag
     4441 aaggctttga tattattatg tcatctttta cccaagaaaa agactacata atatgtgaaa
     4501 atcctactca taatactgct ataaaaatta tgaaatctca taacctcaat atcttaggtg
     4561 tagatataaa tgaagatggt ttggacttca atatgctaga agacaattta gttaagtata
     4621 aaaataaaat aaagtttgca tacattacac cctcttatca caatcctaca ggaattgtaa
     4681 tgactcctga aaataggtac aaattctata atcttatgaa aaaatacaat atagcaatta
     4741 tagaagatgg atttaatgaa gaattactct acaatagttc tcatatattt cctatctgtt
     4801 ccctagataa tatgaataat ggggtcgtat atattggtag tttttcaaaa attttatttc
     4861 ctggaatgag aattggatgg atatttgcag ataaaaaagt aatcaataaa ctagaaagtg
     4921 taaaaaggtg cagaaacata catgtatcct ttttagacca aggaatttta tttgattatt
     4981 taagtaatgg aggttttgaa aaatacatta aaaaaatacg taaattctat ggggacaaat
     5041 ttaactttgc atataaatgt ataaaaaaat atataaaaac agaatatatc ttgggtgatg
     5101 gaggtttgca tatatttata aaactaaaaa atatagatac tagagtttta ttagaaaaat
     5161 gttatgaaaa aaatgttata tttatgcctg gagatttatt ttttacagat aactctggtt
     5221 ttgatacttt aagactcgga ttttctaggt tgtcttttga agacattgaa attggaataa
     5281 aaataattgg agaaactata aatgaaatgt tctaggttaa ctaaatattg ttagcctagt
     5341 ttatttttat gaggtataat atatatagtt tatatgaatc taatactggg ggataatatg
     5401 agtaaaaagt caaaacgaag aaaaattaat aaattaaaat taacgattgt aataactgta
     5461 ttatttcttg ctttattatc tatatatgag atactgttct ctaatcccaa caaaaaaggt
     5521 aatataaaga gtcaagaaac tatgcagaat aaaaataata caacagatga ttctaaaaat
     5581 aatcaaagct ctaccaaaaa gcaggaagat aataatatca agcctacttt ttcatattct
     5641 tcaataccag ataatataaa aaataaaatg attggtaaat ctatgcctac tgatgaacct
     5701 ataagctttg atagtctatc ttatttaaaa cttacatatt atggatttga tgaaaaaact
     5761 catcaaggag aaatgatagt aaacagtgaa ctagctccag aagtagtgga tatttttaag
     5821 gaattatacg aaaaaaaata tccaattgaa aaaattaaat taatagatga ctatgatgct
     5881 gtagatgaaa aatctatgtc tgataataat acctcatcat tttgttatag aactatagct
     5941 ggtacaaatg tagtttcaaa tcatggtaaa ggacgtgcta tagatataaa cccattacag
     6001 aatcctcaag tttctggaaa tgatgttaca cctaaaataa gtacagttta tgctgatagg
     6061 tctagcacaa aatttggaat gataaaaaaa ggtgatgatt gttataatgc ttttgtaagc
     6121 agaggttgga gttggggtgg ctattggaaa aacccagatt atcaacattt tgaaaaataa
     6181 tagattagaa ataaaggtga attatatgaa tttaatgaca ttggaaaata taagtaaaag
     6241 ctattctgaa aaaaaattac tagaaaatat atctcttgga ataaatgagg gggaaaaaat
     6301 aggacttatt ggtgtaaatg gtactggtaa atctacctta ctcaaaatta ttgcaggagt
     6361 tgaagaaagt gagactggta atataacaaa aactaatggt attaggatag aatatctacc
     6421 tcaaaatcca acttacaatg aaaattcaac tgtacttgaa caagtattta atggaacttc
     6481 tgaagaatta aagctacttg gtgaatatca agaaatccta gaaaaactta attcaaactt
     6541 tacagaggaa ttaaataaaa aattattatc agtacacgaa aaaatagatg ccttaaatct
     6601 ttgggattta gaaagtgaag ctaagtcagt acttacaaag cttggtataa atgactttaa
     6661 tcaaaaaata gcagaacttt ctggtgggca aagaaagaga gtctctctgg catcagccct
     6721 tattacacct tgtgagtttt tgatactaga tgagcctaca aaccatctag acaatgatac
     6781 tatagattgg cttgaggaat atcttaattc aagaaaaggc tcattgctta tgataactca
     6841 tgatagatac tttttagacc gtgttacaaa tagaattatt gaactggaca agggtagatt
     6901 attcagttat gatgggaatt attcaatatt tttggaaaag aaaatggaaa gactttcgct
     6961 agaagcaagt attgaagata aaagacaaaa tttaataaga acagagcttg cgtgggtaaa
     7021 acgaggagct caggctcgta gcactaaaca aaaagctcgt ttacaaagat ttgatgaact
     7081 tatgaataga gattctttta aacctgatga aaatctcgat atatctgttg catcaagtag
     7141 acttggaaat aaaataatag aaatacacaa tatatctaaa tcatttgaag aaaatactgt
     7201 tatagataat ttagaataca ctttaactcg tacagataga attggtataa taggcaaaaa
     7261 tggtatgggt aaatctaccc ttataaatat acttaatgga gaaatagagc ctgatagtgg
     7321 atatatttca attggagaaa cagttaaaat aggttgtttt tctcaagacg actctcatat
     7381 gaatattgat atgagggcta tagactatgt aaaagaagct agtgactata tagaaacttc
     7441 agatggtact aggattacag cttctcaaat gtgtgaaaga ttccttttta atggtactat
     7501 gcaatatact ttgataggta aattatctgg tggtgaaaga agaagactac atcttcttaa
     7561 aatccttatg acagctccta atgtgcttct tttagatgaa cctacaaatg atttagatat
     7621 tgaaacgctt aaaatattag aagaatacct agacgaattt aaaggtgtag tagttacagt
     7681 atcacatgat agatatttct tagatagaat ttgtaataaa atatttgcat atgaaggtaa
     7741 tggaaaaatt catatctata caggtaatta cagtgattat cttatttata gagagattca
     7801 aggaattgaa tttgaagaat ctaaaaggga atcagttaaa aaagatgact ctactaccct
     7861 aaaaaaagaa aaacctaaaa atgataaaaa gcttaagttc tcatataatg aacaaaggga
     7921 gtttgaaaat attgactctg atatagaaaa actcgaatct aaaattgcaa cacttgatga
     7981 aagtactgca aaattttcaa ctgattttac taaacttcaa gaaattttag atgagaaatc
     8041 taaactagaa aaggaacttg aatacaaata tgaaagatgg gaatatttaa ataatctagc
     8101 tgatgaaata gctaataata agtgatatta aaataatctt aagtttaata agttttgctt
     8161 taaatctata ctttataaat attttaaagg tagtgtctca aaatgaactt tttagtttta
     8221 tgagacactc ttttttatga gaaatcaaat atgttttcat catttcaaca atacaaaaga
     8281 ggcttatctc tattttgaga cagcctcttt cataattacc ttctaattta tatgctttaa
     8341 tatatctata ataattctta ttatatctat atatccagtc tttcaatata gtgacaatta
     8401 acttcaatat gatttaaaaa tactaacaac ctttcataaa gtggatgaga ttcttctcca
     8461 tatttagctt ctaggttctt gcctatttct ctaacagtcc tttgaccatc aatttgtaaa
     8521 aaaacaaaac tggaatattc atctagttct atttttttgt atttaggtat tctagctttt
     8581 aactttctaa aaaacttttg aattttatgg tcctgttttt caagtatagt aacaacacta
     8641 ttttcagcta cttcatattc aagtccatca gtaattttaa atacaatatc taaaacatca
     8701 ttactcttgc tcattatttt ttactttaga taaaacaata ggtaatgttg taagcacaac
     8761 taaaactact aataaagcaa ttgccattga atttgttgca gcaaatccac ttggtacctt
     8821 tggagttaca actccagtta cttgtaatat tatacctatt aaaccaatga tagaaccacc
     8881 agcaacaaga ccagaagata aacttatacc attagaaact ttaacttctt tttctttttc
     8941 agttttagat actaattcaa caaataaacg aattaatgct ccaactaata ttatagaagt
     9001 tgttgcaatt ggtaaataga atcctatagc tatagtcatt attggtaaat ttaatgcata
     9061 tagaactatt gccataaata ctccaacaat aatcataacc caaggtaaac tacctgacat
     9121 aataccagaa gttaatgttg acattaagtt tgcttgtggt aatgcaaatt gtacattatc
     9181 accagttgct ctaagttggt cagaaagtaa taaaattact cctacaacaa ctataacacc
     9241 aactatactt gcaattgtaa agtaacgttg catttcgttt ttgctaccac caataatata
     9301 agttactttt tgagattgag tataacctcc agctatagca atagcaacaa ctataaatga
     9361 accaaataaa agtaatgatt tattagcttc taaatcagtc catcccatta taacaaacac
     9421 taatgttaca ataactagag aagctatagt cataccagaa actggaagat ttgatgttcc
     9481 aattgttcct gttaaacgac cagctactat gacaaataat aaagataata aaagagatat
     9541 gatagctcca ataattgcca taacaatgtt accagaaatt aagaatgctg ctaaaaatcc
     9601 aataactact ccacctaata aaagtatcat ttgtatagat gaaccttctt ctccttctcc
     9661 agcacttgat ttagctttaa gtgtttcttt tatagaagcg attatagttg gtataagttt
     9721 tattgcacca ataatacctc cacaaagcat cataccagca cctatatatt ttacataact
     9781 actagatatt gctccgacat ccatttggtt aagaggcatt gcaggattat tccataccat
     9841 agctccatct ttagccatat ctgtaaagta tcctattaaa ggtgctatac caaagtttga
     9901 taaaatactt cctgcaaaca ttgttaaaga aacttcaagc cctactataa atccaattcc
     9961 taaaagtaga ggatttactt caatatcaaa tttccatttg tagaatttgc ttcctacaag
    10021 actcattaca ttgtttgcta catttaagaa tgaaccagtt aatacagtaa taaatccact
    10081 tattacaaat ccaattccca tgtacttaat tgaatctcca ccagcctctg aagcaacaag
    10141 tgtctcagat atagccattg attctggata cataagttta ccatgttctt caactattag
    10201 atagttgtga actatagcag caattcctat accaaataaa actccaccaa ctccaacagc
    10261 taggccttca aagaatgtaa tttggcttcc aattaatata actgctggta atacgaaaat
    10321 aaatccactt gcaacagatt ctccaccact tgacatccct tggattaagt ttttaccaag
    10381 aatacccttt gaacgagcaa atgctccaac aaaagcagaa cctattatag ctcctggaat
    10441 acctgcagca actgtaagac cggctttcat tccagagtaa gttgtagatg cggcaaaaat
    10501 ggcagctaaa ataataccga taatcagtac agctacattt ccaccggttc tggacttatc
    10561 tgtgatatat ggaacgtaat cttttccact aacctcacca taagcaccct taggtaattt
    10621 tttgttcata aaattttcac cccttttcaa tttgtatata ttataacata tatacatata
    10681 aaacttaact ttttattatt ttacaaatag cgagtcaaaa ctactaaaat ttaaccctat
    10741 attattaatt tctactttta gatattttta tgctaatttt ctttttattt ttcaattcta
    10801 ctaaaaacta ttaagatata aaataaaaaa aatttttaat gtaaaattaa ttttgtaaaa
    10861 aaaatgcttt aaaacacgga aaagtataaa aaatacaata atcattctta taaataaatt
    10921 ttcaccatag taaatatata cagaaaattc ttttttttac atcaattgat ttcctcagaa
    10981 aaataggtgt cataaagaaa tgtatggaat cactttacaa cacctgtcat aataaaaaat
    11041 cactctggta gtgctttttt atttactttt cctattggtg taactggtat tttatctatc
    11101 ctcattatta ttgttggaat catatatgga ggtagatgct catttaaata ttctctaatg
    11161 gttctttcat ttatcttacc actatcttcc tcatcaatta cataatatgc acatatatac
    11221 atatccccgt catcatttgt tctcgatata caaacaacac ttttaatttc tgtaatatta
    11281 tttattatat tttcaatctc atatagctct atcctaaact catcaatttt tacttgattg
    11341 tccatccttc caatataatc taaatttcca tcatctaacc atcttactaa atcaccagtt
    11401 ctatacatta acttattata ttcacaagtt ttgtaaggat tctctacaaa tacttgctct
    11461 gttagccctt ttttatttaa atatcctctt gatatttgaa tgcctgatag acatagctct
    11521 cctggtgctc caattggaca tagtttgttc tttttatcta atacatatgc ataagtatta
    11581 aatataggtt ttcctattgg tatttttcca tattctctgt ccacatgaaa agaagtcgat
    11641 acaacagtaa attcggttgg tccatactca ttatatagtt cataattacg ctttctatat
    11701 ccttttaatc tttctcctcc agtctgtaca actctaagtg aattattatt gcaattttgt
    11761 ataaataacc ttgcaaatgc tgttggcaat gctagtatag ttatgtcatt ttcctcacag
    11821 tattcattta ctgtatattc atcaaattta gcttcttcag gcataatatc tattgatgca
    11881 cctacaagca aataagggaa tagttgtttc attacagcat caaaactgaa acttaagtat
    11941 ataccacata tatcattttc tgtagcgctt atagatttta cgctataata acacatattt
    12001 actaaactct tatgttctat agctatggtc tttggcctac cagttgaccc tgaagtagat
    12061 atcgtatatg cacaatttga tgatgttatt ttttcagtta attcatatcc aagcttaaac
    12121 atttcttcat catttatatc gagtactatg gtattctcat ttacaacctt acttttgagt
    12181 tcatctgtag ttatcactac ttttgcatta ctatcttcaa gaaggtaatt caatctttct
    12241 tctggattac tcgtgtctat tggaaaaaat gctccccctg atttcataat ccctattgct
    12301 gtaataatta tgttaatatc tcttggtaac attacagcta ctatgtcttc actttccact
    12361 cctatatgat ttaaataacc tgcaagttta tcagttacct catccaattc tttatatgtt
    12421 aactttctat tttcatccct agcagctata ttactaggtg ttaccttaac ttgctcccta
    12481 aataaatcaa cataactttt atcatagggt acttcatatt gattattatt aaagtcttcc
    12541 aataatactt tttcttcttc cttacataca aattctaaat cttctattaa aatatcatta
    12601 tcttctatta tttgctctat tatataatca aattgattta aaattcttct aatagtttct
    12661 cttttatata tagatggttt atactttatt tttattttaa gtgccctctt agtatatact
    12721 attacagata aatcatatcc ttttttttct atgtcataat catatgataa atcaaaactt
    12781 ttagctataa atattgtgtt tataagctta gtatttagat gatttttatt ctctatatct
    12841 gataacaaat agtatccata ctctaaagac ctgctccact gcctttttag cttccttaca
    12901 acatctataa atttttcact ttgtctaact cttactctta ttggaattat attcattgtt
    12961 ttaccaaaaa ctacatcatc ttcatatata tacttttgaa gtataaggcc atatatagtt
    13021 tctatgatag agtctatgct aatattgttc actttagaat attgaattaa cctttcatgt
    13081 ttctcaatat tcatcttaaa aacctcatct actacttcat cagaacaatc tttataaaaa
    13141 aaatcatttg gaatttttgc tatattcgta taatcatcta acaaatccat ccaataattc
    13201 atagctcgtt ctaaattaac ttcatacata tcaaaattcc cccttactac taagtctata
    13261 gaaaaaattc ttttggaatt ttattttcac aatatataaa tatattaata atacatatgc
    13321 aagctctaaa aacatgagct tgcataaaat tacacttatt atgactacta aattcgtaat
    13381 atttacaatg tttaaaatat attataacac atatatgatt ttatttttct acatttatat
    13441 aatatataaa ttacaaatag ttatttaact aatatataac aatacctttt ggaatatgat
    13501 atattatatt aattaaattt ttataagata agttatactt ataggaggga accatataaa
    13561 tgaatattta tgctcatagg gggtttagtg gaaagtatcc agaaaatact attctagctt
    13621 ttaaaaaatg tttgtatatg gatatatatg gaatagaact tgatgtccac agaactaagg
    13681 atggaaaaat tgtagttatt catgatgaaa aggttgatag aacttttaat ggacatggtt
    13741 ttgtaaaaga ctttacttta agaaaattaa aaactctaaa ctcatctttt gaaggatatc
    13801 aaaacaataa agaatgtaaa attccaactt tagaagaagt a
//
LOCUS       DAEPLF010000082        13756 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEPLF010000082 DAEPLF010000000
VERSION     DAEPLF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.082"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            134..1060
                     /locus_tag="KR314_002070"
     CDS             134..1060
                     /locus_tag="KR314_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBH0328453.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTVLYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(1180..2178)
                     /locus_tag="KR314_002071"
     CDS             complement(1180..2178)
                     /locus_tag="KR314_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBH0328454.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMTGKHVGRTVVDLEKI"
     gene            2282..2719
                     /locus_tag="KR314_002072"
     CDS             2282..2719
                     /locus_tag="KR314_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0328455.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            2805..3392
                     /locus_tag="KR314_002073"
     CDS             2805..3392
                     /locus_tag="KR314_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBH0328456.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(3692..4312)
                     /locus_tag="KR314_002074"
     CDS             complement(3692..4312)
                     /locus_tag="KR314_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBH0328457.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(4320..5087)
                     /locus_tag="KR314_002075"
     CDS             complement(4320..5087)
                     /locus_tag="KR314_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBH0328458.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(5128..5949)
                     /locus_tag="KR314_002076"
     CDS             complement(5128..5949)
                     /locus_tag="KR314_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328459.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(6306..8216)
                     /gene="phnW"
                     /locus_tag="KR314_002077"
     CDS             complement(6306..8216)
                     /gene="phnW"
                     /locus_tag="KR314_002077"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBH0328460.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(8495..9280)
                     /locus_tag="KR314_002078"
     CDS             complement(8495..9280)
                     /locus_tag="KR314_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH0328461.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(9497..9727)
                     /gene="dltC"
                     /locus_tag="KR314_002079"
     CDS             complement(9497..9727)
                     /gene="dltC"
                     /locus_tag="KR314_002079"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBH0328462.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(9746..10894)
                     /gene="dltB"
                     /locus_tag="KR314_002080"
     CDS             complement(9746..10894)
                     /gene="dltB"
                     /locus_tag="KR314_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBH0328463.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKHISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(10894..12408)
                     /locus_tag="KR314_002081"
     CDS             complement(10894..12408)
                     /locus_tag="KR314_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBH0328464.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVIFN
                     FSDERNFGDINVIDMDKLNDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLREEI"
     gene            complement(12529..13719)
                     /gene="dltD"
                     /locus_tag="KR314_002082"
     CDS             complement(12529..13719)
                     /gene="dltD"
                     /locus_tag="KR314_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBH0328465.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGINPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
BASE COUNT         4531 a         2390 c         1456 g         5379 t
ORIGIN      
        1 ttttaaatca tgatgtatta ttttaaatat acagttacat tattattcac aatataagta
       61 tcttagttct caaataacat ctcatgtaat ctattcaatg caaaaaattt aaatattttt
      121 taggaggttt tttatgagca aggaaaataa aaatgtaaga attgctgttg tccaggcgtc
      181 accagtcata atggatttag aaaaaactgt tgaaaaagca ttaggattaa ttaaagaagc
      241 tggtagaaaa ggggcaaata tcgtagtttt ccctgaagca ttcatacctg catatccaag
      301 aggtctttca tttggatttg ttgttggaag tagaacaatg gaaggtagag aagattggaa
      361 aagatactat gataattctg taccagttcc tagtgctaca acagatttat taggaaaagc
      421 agcacaagaa gcaggagtat atctatcaat gggtataaca gaaagagatg gtaatgatat
      481 aaattgtacc ctttattgca caaacttatt tttctctcca gaaggtaagc taattggtaa
      541 acacagaaaa cttaaaccta caggaacaga aagatgtatt tggggagaag gagatggaag
      601 tactcttaca gtagttgata ctccatatgg aaaaatgggt tcgttgattt gttgggaaaa
      661 ttatatgcca ttagctagaa cagttcttta tgctaagggt gtaaaacttt acatagcccc
      721 tactgcagat tcaagagaag agtggcaagc aactatgaag catattgctt tagaaggaag
      781 atgttttgta attggatgca atcaatatgt tgaaaaaaat atgtatccaa ctgaccttaa
      841 ctactataag gagctagatg cagaaccaga aattatgtgt ccaggaggaa gttgtatagt
      901 tgaccctttt ggaaaatatg tagcaggtcc tattttcaat aaagaagaaa tgctaatagc
      961 tgatttagac ctagaaaaaa tagtacttag tagactagat tttgattcag aaggtcacta
     1021 ttcaagacca gatgtatttg aattaattgt tcatgaataa tctttttaca caaacatcta
     1081 cataaatttt tttacataaa aaaatgagat actcataagt aaatttcaag tatctcattt
     1141 ttgcttaaaa cctataatcc taattataaa tttattcgcc tatatttttt ctaaatcaac
     1201 tactgttctt ccaacatgtt ttcctgtcat tatcgaatct atactatcat ttaactcttc
     1261 caatgatact acatttacca tatcatctaa atcatctaat ttccattcat ttgataataa
     1321 atcccatatt tttaatctta agttcattgg gcaatccaca gaatctatac catataaagt
     1381 aattcccttt aagataaatg gatatactga acttgtaaaa tcaactcctc ctgcattacc
     1441 acaagttgtt actgctccac aataattagt tgtctttatt gctgttgcta gagtatttcc
     1501 tcctactgta tctattactc cagcccatct tggtctaagc agaggtcttc ctgagttatc
     1561 atccacttct gacctatgta taacatcttt aacacctaag tttagtaata cttctttttg
     1621 ttcttctaat tttcctgttg aaactacaac tgaataacct aatttaatca atattttagt
     1681 tgcaattaca gcaactcctc cagtaccacc agtgactaat acatctccca tattcttgtc
     1741 tacagaatct ataaatttat acacagataa agctgctgta aaccctgctg taccataatc
     1801 cattgcttct tttagtgaaa gatttttagg aagtttaact acccaactag aaggaactct
     1861 tatgtattct gaaaaaccac cagaagtatt cattcccaag tcataacctg ttactaaaac
     1921 ttcttctcca attttaaaat catcacaact actagattct actataccag atgcatcaat
     1981 tcctggtgta tgaggatact ttctcgtaac acccttatta ccatttgcag aaagtgcatc
     2041 cttgtaattt agagaagaat attttacttt tattagaact tcgccttctg gcaatgtatc
     2101 tacttctctt tcaactatat ttcttctaaa atttccttct tcttctgtaa tatatagtga
     2161 tttaaatttt ttattcataa atttcctccc aatacattta gttttatgtt aaactaatta
     2221 tagtttataa atagttttat gtcaaactaa tttaaataga ttaaagattg gagaataatt
     2281 tatggaagat aaatatattg tacatttcat aagtaaaaca aaggctagta tgattaaatt
     2341 cattgaaaat aaactcagta aaaatggttt aggtgagtta atacctactc atggaaatat
     2401 tttaactgca ctttatgaaa ataatggaat acttacaatg aaagaaattg cagctaaaat
     2461 aggaaaagat aaatccactg ttacagtgtt ggttaataaa cttataaacc taggatattt
     2521 agaaagacaa aaatgtacaa atgacaaacg tatcacgtat ataaagctta caaaaaaggc
     2581 actgcttata gaagatactt ttaatagcat ctcaacacaa gttaaagaga cagcatacca
     2641 taatattaca gaaaatgaaa aacaagaatt tcttagaatt ttaaaaaaaa ttaatgataa
     2701 ttttaaaaat gctgactaaa ttgagcataa ttatatctcc tattggaata ataaataaaa
     2761 agaatcaaat attgtaaaaa tttattatga tagagaggta gaatatgaat ttaaaagata
     2821 gtaaaactaa agaaaattta ttaagagctt ttgcaggtga gagtcaagct agaaatagat
     2881 acaatatttc tgcatctgta gcaaaaaaag aaggattata tataatagag tctttattta
     2941 attacactgc tgaccaagaa aaatcacatg caaaacaatt cttttctaaa ttaaaagaat
     3001 ttgctggtga aaatataagt atatcaggag cataccctgt agatttatat gacaagacag
     3061 cacaacacct taaagctgca caacataatg agcttcaaga gtgggatgat gtatataaaa
     3121 actttggaaa aacagcacgt gaagaaggat ttgaatctat agctaagtta tttgaaaata
     3181 tagctgaaat agaaaaagtt catggtgata gatttggtag atatgctact gatttagaag
     3241 aaggtacttt attcagaaga aatgaagata tacaatggat atgtacacat tgtggccata
     3301 tacatatagg taaagaagct ccaaaagctt gtcctgtatg tcttcatcca caaggatatt
     3361 tcttagattt taaaaactca ttatttgaat aaactaaggc aataattcta tttaaacaat
     3421 ttactatatt tatatcaagt atttttataa atactgtatt atcttaataa caataaaatg
     3481 aaccattgat attactataa gtagaaaatg tagtttaaat acaatttata gtaattcatc
     3541 atggttcatt ttattattta ctaatcccta taacctactt aaatctatct ttaactttta
     3601 catactcaca taaaatctta tctttaaact aaatatttaa ataaagtgta ttttaagtag
     3661 attattataa tacattatat aagataatac actatgataa ctttatactt atatcattta
     3721 tttcttttgt caagtcaatc tcaataatct taactacatc tttttcaaac tttacttttt
     3781 tataatccag acatcctact acgaatggaa ctccaaactt tctaaacaaa taaatctttc
     3841 cactttcatt gtatattgat gtaaaatttt cttctattat attcactatc aattttaact
     3901 cttcaaaagt tattatagat ttaactaaaa acttctctat tatgctcaat ataagcagtt
     3961 cttttttacc tatgctttgc tcttttttat ataaattttg aaatatttct ttggtataat
     4021 tacatataac ttctttactg agaataaaat caatgtcaaa ttccttatta atatcctttt
     4081 tcataaacat atttgcaata tcatcaagag aagtctcttc tttcatagac aatattaact
     4141 ctattctttc tagtatttta tctcttggaa agaaagtctc ttgacctgta aaagctgatt
     4201 tttttataaa ccattcctct ggtattatgt tttttctttt ccatctatat aattgaccat
     4261 aagatatatt agttttgctc aataaatctt tcttagatat taattcttcc ataacttttc
     4321 tagcccacct ttatactgtt ttttacagta gaattatctg aaactgataa attatcactg
     4381 tattcaatat ttccaacgtc acaattatct actagagata tgcttttgcc tcgtatatct
     4441 tttattgatg catcttctaa ttcaatagta tctccctcta tcaaattaca tttaaacttt
     4501 cctgaaaaaa tattaatgat tttaattaat gaaaaagcat ctttgcttct tcctattttt
     4561 atattagttg cccctatctc tttacaataa gaacctcctc ctgaataaac atatatattt
     4621 tctccattta aaagaccatc acattttatt actccatata cctttatatc ttcacattct
     4681 atatttgatt tatttctcat ttcgccatag atttttatat cttgtgcata catattttca
     4741 tttacactta attgtcctct tattgtaaca ttattacatt taagtttgcc atccactttc
     4801 tgttgagcag ttataactaa gtcatctatt tctgcatctc cctctatatt aagttgacca
     4861 ttcactctta cacgctctgc tttaagattg cctcttataa tagtttttcc gttaacaaca
     4921 aaatttttta tattgatgtc accttcaaat tttgcatctc caaggacttt tacttcgtta
     4981 cttttaacat ttccttttga tttagaactt cccacgattt ttatattttc atactcacca
     5041 ctacttataa agccttctcc aagtattttt atagaccctt tttccatatt attacctcat
     5101 tttaaatttt atttttaaac ctatttatta cttatccttc tttaatttta ttttttcact
     5161 tatattcttt atttcaccaa ctattgaatc ttcatcaatc tctatacttt cactgtactc
     5221 caaactttct acataacatc ccttgcctat tttaatattt tttcctctta caactttgac
     5281 ttcactattt tctaagctta tttcatctgc ttctataata tttgcaaaca atttactttc
     5341 tgtatacttt ctaattgata atagacctaa acttattaaa tttgaagaca ctttttcaaa
     5401 gttgatatgc tttttttcta gcatacgaaa ttttttgcaa taatctccat gccccttttt
     5461 tatatttata ctacttgctc caacttcatt aaaattagaa gttccattta aactaatatt
     5521 aacttcttca caattcaaaa atccattaca ctccaagatt ccacttaaat tgatattttc
     5581 acaatctata ttctcatttg cttttaaata tccttctcca tgaataatat ccgattttat
     5641 acttccttct acattaataa ttccactagt atcaatttca acagaattaa tatttcctgt
     5701 acatctaaga agtcccgata tctcaacttt tgatgccagt atgtttcctt ctactttact
     5761 taaaccagat gtaaaaaatt cattacattc tgttgtccca aaaacttttg ctgaaccaga
     5821 agaatcaaat ttactacatt ttacacttcc ttctattttg cctgttcctg atatattaac
     5881 attatcaaaa attccacctt tacattttcc aacaccatca aatcttagtg aacttaattc
     5941 ttcattcata atgatatcct ttctttaccg tattttatct ttaaacctaa tataacaaaa
     6001 cattgttacg ttgtcaagtc tgataataat acttaataaa gcaactatca tttattttct
     6061 ataaataggc ataaaaataa tagggaaatg ctttatttaa actagaaatc taatttctta
     6121 gattgtggtt taaataaaat atatccctat tactcaatct ttatatattc attgtataaa
     6181 cttctaaaat ataagtcttt aaattctact gtcaatactt tttatacact gtttgtgcta
     6241 tcatttcaca cctaaaggag taggctctcc gcactattct gtaacctaaa agacactatc
     6301 ttcttctatc tattgataaa tttttctatt acatcagcta gtctctccat atcagttgga
     6361 taaacttcac ctatgctacc tattctaaat gtatctatat cagtcaattt acctggatag
     6421 ataacaaatc cattatcttt taaataagta taaaattcca taaattcaaa ctttgcattt
     6481 ttaggatata agaaagttgt tattacaggt gattgagcat tatcattcac caaagtatca
     6541 aatcctaaag attttaatct tgaagctatt gtaaactgat tttctttata tctagcatat
     6601 cttttttcaa cagaaccttc ttcttccaat tctaataaag cttgataaaa agctcttaca
     6661 acatgtgtag gtgaagtaaa tctccattta ccattatttt tctccatagt ctcccattgc
     6721 gcatacacat caagagataa tgatttagct atacctttac atttagataa tttctcttta
     6781 ttagctatta taaaaccaaa tccaggtact ccttgtatac atttgttaga actactaact
     6841 aaaaaatcta tattaaaatc ttcaacatca atctcaattc ccccaaaact actcatagca
     6901 tcaactatat aaattttgtt atattctttt gcaagtttcc ctacctcttg tataggattt
     6961 aatctacctg tagtagtttc acagtgaacc attgatatat gtgttatatc cttgttttct
     7021 tttaataaat tttctactac atttaaatcc actgcttcta tatctttaaa tgtgcagtca
     7081 acaaattcta tgttaagata attacatata tctttcattc tctttccata tgctccattt
     7141 gcaattacaa gaagtttacc atcttttgaa atagtacttc ctattatagc ttctactgaa
     7201 aaagtcccac ttccttgcat taatacactt gtgtatttat ctgtattttg ggttgctaga
     7261 gatactaatc ttcttctaac atcttgtact aggttattat attcaacatc ccaagtacac
     7321 caatctttta acatagatgc tcttactgat ttagtagtag atagtggtcc tggtgtaagt
     7381 aatatgtaat cattttctgg tacaaaatct gatttaatag tttcattctc tatttttatt
     7441 agtatatctt ctaactcagc catagtttct attacaaaat gtgcacctgc ctctttaaat
     7501 ttctttgaaa ctatactcat cttagctttt aattcttcct tatccatgtt ctctacttct
     7561 tcttgtgtaa gccctaactc acttgaacct tttattacag caacactcca cattcctgca
     7621 tttacgcctt cttttacatc acttattgta tctcctactt ttaccataga actcatagga
     7681 gacaccccta aagcttcagc atttttatag cacatccaag gatacggtct tccttgagat
     7741 acttctgatg gtgttacaag aaaatcgggt gagtatcctt tctttgctgc atttggttca
     7801 actatgttca tcatctctct tgtataaccc gttgtagaac ctatttttaa tccatttttt
     7861 ctcaactttt caatagtttc aacaacatga ggaattggag tcgtatactc ttctaatgtc
     7921 tcaaataaca ttggctcaaa ttcagcataa agttcattta catcatcttc agttggcact
     7981 ttaccaaact tatctgacca aagatttttt attctatcca tttcacacat ttctcttata
     8041 tggtctattt ttaattttcc cataggtttt cttgcttctt ccattgttac atctatacct
     8101 cttcttttaa atatctctat aaatacatta agaggtgcaa aacatccata atctactgtt
     8161 gttccagccc aatcgaatac tactgctttt attttttctc catatatctt tttcatattt
     8221 atagcctcca tagattcatt ttagaatttt tgtgttttta attgttttta cttttaaagt
     8281 ataactttca tatgttaatt atacatcata tttaggttaa atatatatta aatttctgtt
     8341 tttagttgta tttaattgtt tttattttat atcatatttc cataaaatcc taagttacaa
     8401 tctaaaattt actttctaat cttaatctat gaactgacta tattttatga atttagttgt
     8461 attaagcata gttgtattat ataaaactaa gggtttattt tgcttctatc catgttatat
     8521 ttatctcatt taacttatcc ttccattcat tcggaggttc tacatcacaa ataacaaaat
     8581 ctaattcctc taaatctgct atcttataca tactagcaat tcctatttta gaactatcac
     8641 ataatacaaa actttccttt gaatttttta tatattcttt tgacaacaat gccttatttt
     8701 ggtctatact actaattcca aaactatcaa gtataccttc actagatata aaagctttat
     8761 ctacgtataa gtttttcata gtgtctattg acattggaca tgctgtcctt tggtgtttta
     8821 aatttacttt tccaccaata aatataactt ctccatcaaa caaaccttga ttctcataag
     8881 aaatcaattt tgatagaact ggataagagt ttgtaataat ttttaaattg tttacagata
     8941 ttatataatc tacaatctgc aaattggtag taccttcatc tatgataatt gactcattgt
     9001 catttacaaa cttagctgcg tatttagcaa tactttgttt tttatctagg ttagtattgt
     9061 ttctctttat atgttctggt tcaatcactc cataatttat ttttatagca ccaccatata
     9121 ccttttttaa tttattttca ttttctaatt cttccaaata tcttcttatt gtttctgatg
     9181 atacttctaa tctttttact aattctttag tatttacctt cccttttgaa tctaataatt
     9241 taagtatttc tttttttcta tctatcgaat ttaaagacat tcattttcct cctaaatata
     9301 atatattttt ctcctataat attacaatga tacctaagat ttatgaaatc atatttatgt
     9361 attaaatttt ttacttaatt aaagttattt tcaaaagact ttgtattatc attaaaaaag
     9421 tccatcaaat attagtatca gatggacttt gtaaaatttt attttataaa ttaatattta
     9481 aagtctaact actaatttat tttcttgtct ccaagaattt tactaaatta tttacagtag
     9541 acatatcttt tctctctaaa tcagttggtt gaagctctat ccctaatcta ttttcaattt
     9601 caagtaaaac ttcaattata gctaatgagt ctaaaagttc cgtctcaaat aaattaaggt
     9661 ctaaatcttc tcttatctca tcagtcccta atacatcttc aaatatttct ataactgttt
     9721 cttgcataaa ttttcctcct taaaattaag ctaaataacc tgagaatatc agcaatccaa
     9781 aacacacaat atggaatgta atgaaaatct gcactctctc gaaccattta tcttttttat
     9841 gttttttata aaatttagat tttcgctgat atatatcagt taacacaagt gccaatcctt
     9901 ggtatacacc ataagcaaca taataccaag ttagaccatg ccaaaatccc attgttatca
     9961 tggttatcat ctgagccaca tgagcagcag tagtcctttt cttaaatctt ttctttctca
    10021 ttgatgacat aacaaatcta gaaaaaatat aatctccaaa ccatcttgaa agacttatat
    10081 gccatcttgt ccagaactct ttcatatctt tactgataaa tggcttgtca aagtttatag
    10141 gtacttgtat tccaaatatg taccctgtac ctatagcaaa taaactatat cctgcaaaat
    10201 caaagaacag gtataaacta tatgcgtaca tataacttaa aatatttata aaactcatat
    10261 ctttaggtat tcttgatacc caatatgtat ttatcaaaaa tgctataaca aacttataac
    10321 ctactcccat gacaatattt tttattcctg gcagtaagta ttcttctata tactcttttc
    10381 tagagatgtg tttttccaag tcctgttcaa atcttcttga cctatcaata ggtccagaac
    10441 ttaaagttgg aaaaaataac ataaaataaa gcatctttga aattttaact tcttttatag
    10501 ctccatcata aatttcaata accatttgta tagttctgaa atttaagtat gatattccaa
    10561 taaatccaat tattccaaaa gaagtaactg gagatatttt atttattatt attgggagca
    10621 ttgatgcaaa caagaatccc caatatatgt atttattttt agtttttctt cttacatatt
    10681 catatccttt aactattatt acttcaagaa taataaatat taccaaatac tttagctgga
    10741 catcaattcc tactattaag aatatcataa acaaacttgc caacatacca taatacttta
    10801 tattcttgcc cattagacct aaaattactg ctggtattga tgttaataat aatatataaa
    10861 gataaaaata atctccatac tgtgaaaaaa tcattaaatt tcctccctaa gtttctttct
    10921 gtctatttta ccattgatat ttgttggaaa ctcagagatt attgaaatat ttcttggaat
    10981 catatatgaa ggtatatact ttccaagttc cttttttatt atcatcccat ttttaatatt
    11041 acttaaatca ttcttttcat ttaactcaac aatacctttt aagtaagcaa ttttttcgtc
    11101 cttatatact ggtaaaacta ctgcattttt tacattatgt acttttctca aattattttc
    11161 tatatcttct atttcaattc taaatccatt taatttaatc tgaaaatctt ttcttccaca
    11221 ataatatata tttccatcta aaagatatcc catatctcca gttttataag ctctccactt
    11281 tacaccatct atttcatcat agaaaaatac ttcatcagtc ttttctttat tattgaagta
    11341 acctttagaa actgatggtc caattattat aatttcaccc ttttcatttt ctttaagttc
    11401 atttccatct tcatcaagta tttttatttt acaatttgac atagggtatc caactggaag
    11461 acttttttca tcatctatag ctttttgagt catatcattt acacttacac caacagtagc
    11521 ctcggttggt ccatatccat tgataattct tgtgtttgga aatctactca taagttcttt
    11581 agtaagattt tttgaaagtg cttctcctat gaatatcatt gactcaaggt taggaagcat
    11641 tttactatta aactcttttt cagttataca tactcctgca aaagaaggag ttgacaccca
    11701 tacagctata tctgagatac taaattgtct aaatagttct ttatagtctg ctaatacatc
    11761 ttttgatatt gaaaataatg tagctccatg gataagacca gggtatattg ttgtaactga
    11821 aaggtcaaaa gaatatgctg gttggttcat tattactttt tcacttccat ctatgtttag
    11881 atatgggctt atccaatctg aaaagctatc taaattgttt gaacttattt gaaccccttt
    11941 tggctttcct gtacttcctg aagtaaatag tatatatgca ttttcatcat cttttaccca
    12001 gttttcttta tctaatgatt ttccctgata ttcatttatt atatcattta atttatccat
    12061 atcaattaca ttgatatctc caaaatttct ttcatcacta aaattaaata ttacttttgg
    12121 ttttatctct ttagtgactt caaaaactct atcaattgga aaactaatat caagaggcac
    12181 atacgctcta cctgatttta aagctcctat catacatgcc attatcatgt tttctttatt
    12241 tccataaatt actataggag tatcctcttc tttgtataca tcctttagaa atactgatat
    12301 agcatctgaa tattcattta aatctttata agaaagttta tctccattac acattagtgc
    12361 tgttctatct gtatttgaat atttttttat tccttcaata attttcatat aagaatgctc
    12421 ctttaaaatc cgttataaat aaatggcaat tctttaaatg gagtaaatgt tagaactcct
    12481 atcgccaata ttataaccat agttaaacaa atactagtta acaaatcttt attgttcttt
    12541 aaatatttta aataatctct cacagacatc tacccatcct ttcgtaccaa gatgcattac
    12601 atcccttaaa taatacttat ctgacccttt atctttaagg tttagaactt caaagccttt
    12661 gctttctgct attttttgtg ctttatcgta gtactgattt ctttcttttt cagaaatacc
    12721 agtcaaatta tagaatttat ccatacttgg gattaataca acaactggat taatacctaa
    12781 gtcagtacat acatttagca tcagtttata attttcaaac tccttagatg ttaataaatt
    12841 tacatctttg tctcttcctt tgtattgctc tataccatct ttaaaatgct ctttataata
    12901 gtacttatca atatttagtg gatttttacc aactcttttt ttagcatctt caatggcttt
    12961 ctttctctca tatgaccaat ttattggtct ttttcttttt gttgctctct ttttatctag
    13021 cttaattaat cgtttataaa gaataccctt ttctttaaga gctatacagt actttcttcc
    13081 ttgaaaataa ggctctaaaa gcaccttttc agctttttca ggaaatgtct taggttcata
    13141 tagcttagca tataatgctt ctgctttata ctcatcactt ccccataata gcttacttga
    13201 tttttttgca tattcaatct tattttgttt agatattttt ggattatcta aaaatcgata
    13261 aaattgtatt ggtgaaaatc tagactgata atggtgactt gtgactccgt ctttttccat
    13321 aaaccattgc atagaaatta acaagacaac tttcttatta tcaatattag gattcatact
    13381 tcctaatatt gcagcatctt gtaatgtctg agtataggct ctcccaattg caaacacctt
    13441 atttttactc ctatttgtat taaaatagta ctttggatgt tgttttgttg aattactaag
    13501 ttctgatgac cccaaaatca taatatcctt ttctcttaaa aaatgattat ttgctgtaac
    13561 ccctttatct tttatatcac ttaaagtatc atccataatt ggaagtagat ttttctccct
    13621 caaaagttca tcagttttag aatcaagaaa tttgtctaac ccaaataaaa atactacgcc
    13681 tattataaaa ggtgtaataa agtatatcaa ttttctcata ttttctctcc tctaaaaata
    13741 ttcaaattta tgttat
//
LOCUS       DAEPLF010000083        13395 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEPLF010000083 DAEPLF010000000
VERSION     DAEPLF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.083"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            30..1481
                     /locus_tag="KR314_002083"
     CDS             30..1481
                     /locus_tag="KR314_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBH0328466.1"
                     /translation="MNFLNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1701..2162)
                     /locus_tag="KR314_002084"
     CDS             complement(1701..2162)
                     /locus_tag="KR314_002084"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBH0328467.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGRYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(2268..3461)
                     /locus_tag="KR314_002085"
     CDS             complement(2268..3461)
                     /locus_tag="KR314_002085"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBH0328468.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(3484..4149)
                     /locus_tag="KR314_002086"
     CDS             complement(3484..4149)
                     /locus_tag="KR314_002086"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBH0328469.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(4183..5286)
                     /gene="ribD"
                     /locus_tag="KR314_002087"
     CDS             complement(4183..5286)
                     /gene="ribD"
                     /locus_tag="KR314_002087"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBH0328470.1"
                     /translation="MNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNIIGK
                     GYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKRVVI
                     STLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKSAVS
                     LDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQEKVS
                     HPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSKNNL
                     VDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPTFVS
                     GEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(5418..5539)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5728..8211
                     /gene="recQ"
                     /locus_tag="KR314_002088"
     CDS             5728..8211
                     /gene="recQ"
                     /locus_tag="KR314_002088"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBH0328471.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDLKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GANILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVMLDSYLENLKNNIIAE"
     regulatory      8299..8410
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8552..9853
                     /gene="thiC"
                     /locus_tag="KR314_002089"
     CDS             8552..9853
                     /gene="thiC"
                     /locus_tag="KR314_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBH0328472.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            9907..10101
                     /gene="thiS"
                     /locus_tag="KR314_002090"
     CDS             9907..10101
                     /gene="thiS"
                     /locus_tag="KR314_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBH0328473.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            10094..10897
                     /gene="thiF"
                     /locus_tag="KR314_002091"
     CDS             10094..10897
                     /gene="thiF"
                     /locus_tag="KR314_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBH0328474.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            10960..11730
                     /locus_tag="KR314_002092"
     CDS             10960..11730
                     /locus_tag="KR314_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBH0328475.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            11747..12850
                     /gene="thiH"
                     /locus_tag="KR314_002093"
     CDS             11747..12850
                     /gene="thiH"
                     /locus_tag="KR314_002093"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBH0328476.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYEFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            12851..>13395
                     /locus_tag="KR314_002094"
     CDS             12851..>13395
                     /locus_tag="KR314_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBH0328477.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITD"
BASE COUNT         5015 a         1593 c         2268 g         4519 t
ORIGIN      
        1 caaaactaaa taggggggta attaatataa tgaatttttt aaatggtatt ttaacgagta
       61 ttaacaatgc agtagtattt gtaaacaatt acttatggtc gtatatctta attataatgt
      121 tagttgtaat tggtatttac tttactatta aaacaaattt tgtacaattc agatatttta
      181 tagaaatgtt tagacttctg ggtgatggta cagcgaataa aaatgcaaaa aaggaaggaa
      241 aaatctcttc atttcaagca ttttgtataa gtacatcttc aagagttgga actggaaata
      301 tagctggaat agcaattgca gtggtagcag ggggacctgg tgctgtattt tggatgtggc
      361 ttatagcatt aattggttct gcatcaagct ttgttgagag tacattagct cagatttaca
      421 aagtgaaaga tggtcaagct tttagaggtg gaccagcgta ttatatggaa caaggtctaa
      481 ataaaaaatg gatgggtatt ttattctcaa tacttataac tatatgttat ggttttgtat
      541 ttaatgcagt tcaagcaaat actgtttcac ttgcatttaa taatgcattt ggaataagta
      601 aaatggctat gggaataggt cttggaattt taactgcaat agttattttt ggtggagtgc
      661 atagagttgc aaaagtttct gaaataatag ttcctgtatt tgcaggatta tatatattag
      721 ttgctttggc tatagttgta atgaatataa cagagatacc aagtgttata gcattgatag
      781 ttgaaagtgc atttgatttt aaaggaatgg cgattggtac atttatggga gttgtaatga
      841 ctggagtgaa gagaggtcta ttttctaatg aagcaggtat gggtagtgct cctaatgctg
      901 ctgcaacagc acatgtaact catcctgtaa aacaaggttt aattcaatct ttaggtgttt
      961 ttacagatac tataataata tgtagttgta cagcattcat ggtattatta tattcaggtt
     1021 atagtagtag tggagcaact ggaattgaat tagctcaaga agctttaact catcatatag
     1081 gaccagttgg aaatatattt atagcagttt gtatattctt gtttgcattt agttctatag
     1141 ttggtaatta ctattatggt gaatcaaata tggagtttat gagtggaagt aagactaaac
     1201 ttaatgtatt tagagttttt gttgtaggca tggttttatt tggttcactt actcaagtag
     1261 atgtagtttg gaatcttgca gacttattta tggcattgat ggcgataata aacttaatag
     1321 caatagcact tttaggaaaa tatgcattta ttgctttaaa agattataca agtcaaaaaa
     1381 aatcaggaat aaaagaccct gtgtttattg cagatgaaat agaaggccta gaaaaggtat
     1441 cagagtggca tagggaatta tctgaggaac atttaggata aaaaatagta taaaattata
     1501 aaaccatttt gaaagactag agtatgtata tgctttatat gaatataata ttgaattaat
     1561 ttatattact ggaattttta attccagtaa tattttttta ttatataatt tagctttatt
     1621 aaaagttttt attattaaaa atagcttatt tatcgtaaag tgaatgatat tggtttttta
     1681 attattatga atgttggtat ttaatttaaa gacttaaata aatttgccat ttctagcgca
     1741 ttcatagcac aatcataacc tttatttcca gcttttgtac ctgctctttc aatagcttgt
     1801 tcaatatttt ctgtagttac gattccaaat ataacaggta attcactgtc taaagatact
     1861 tttgctatcc ctttagagac ttcactacat acataatcat aatgagaagt agcccctctt
     1921 ataacacagc ctaaacagat tatagcatca tacctaccac ttttagccat tttttgagca
     1981 acaagaggaa tctcaaaagc tccaggtacc caaactattt ctatattttc tggagaaacg
     2041 tcatgtctta aaagacagtc ttcagctcct gataaaagtt ttgatgttat aaattcatta
     2101 aaacgagaat ttattatacc tatttttaaa tcttttccaa taagttttcc ttcatatatc
     2161 atttttattt cctccaattt tatttatgtt atagatttat atttataaat atctttaatt
     2221 ttaattataa aaaactactt agtagaaaaa tatcatcata taatcattta tataatatct
     2281 aaaagatgtc ccattttatc ttttttagtt tttaaatata gcttattaaa gtcattaatt
     2341 tcttcattaa tagggattct attttcgatt tttattccat atttctctaa ttgatttatt
     2401 ttatccgggt tattacttaa aagatttata ctttttatgt ttaaattttt taatatttga
     2461 gcagccatat agaaatctct catatcttct tcaaatccaa gcattaaatt tgcttcaaca
     2521 gtatcgtaac cttcttcttg aagtttataa gccttaattt tatttaaaag gccaatacct
     2581 ctaccttctt gcttcatata aattaaaact ccacttccat tttccacaat tttcttcata
     2641 gaagattcta gctgtaatcc acaatcacat tttagagagt ggaacacatc tcctgttaaa
     2701 cattcagaat gaagtctaac tagagtattt tctatattta tatttccata agtaagggca
     2761 atgtgttctt catttgaata agtatctcta tatcctatga tttcaaaatt tccatatttt
     2821 gttggaagga aagcactaga tactttttct atagtagttt catttatttt gcggtattct
     2881 ataaggtctt ttatagtgat gatttttaaa ttatgctttt ctttgaaaat cattaaatca
     2941 tcaactcttg ccattgtacc atcatctttc attatttcac agataagacc aatctcttta
     3001 aaacctgcaa gtctagcaag gtctactgag gcttctgtat gtccatccct gacaagaact
     3061 ccaccagttt ttgctattaa aggaaatatg tgaccaggcg ttttgaaatc tttacttgta
     3121 gaattttcat cacagaacaa ttttacagtt tcagctcttt catatgcaga tataccagtt
     3181 agagtatctg ctccatctat agtaacagta aaggctgttt gaaatgtatc tgtattttct
     3241 ctaaccataa gaggtatatt aagactttta aatttttttt cagtggcagg tagacaaatt
     3301 agacctctac catatgtagc cataaaattt attgattcag gagtagccat ttcagctgcc
     3361 atcaataaat ctccttcatt ctctctactt tcatcatcaa ctacgattac cattttccca
     3421 ttttttatat cttcaatagc ttcttcaata gtattaaaca tgtaattcac tcctttatta
     3481 tttttaaaaa ccatttttaa ataaaaaatc tttatctata tttgatttat ggttactact
     3541 attagaattt aattccttgt agttattagc cataaaatta ttaacatact ttccaattac
     3601 atcgctttct aaattaacaa aatctccaac attctttgtc aagagaatag tgttaatttt
     3661 agtatgaggt ataatagaaa ccttaaatat ttcatcatct aaatatgata tagttaaact
     3721 tacaccatca atagcaacag aacctttgag tatcattgaa gataaaagtt ttttatcagg
     3781 tcgtatactt actaaaactg cattttcatc tttttcaaat ttgcagatag ttcctttacc
     3841 atcaacatga cctgttacta tatgaccacc aaatcttgaa gaaagactca tagctctttc
     3901 aagatttacc ttatcatttg catttaataa acctaaattt gtggaacgaa ttgtctccat
     3961 cataacatcg gctgtaaatt cattttttcc gaggtttgaa acagttagac aaactccatt
     4021 tacggctata ctatctccaa gatttgtgcc atctaatatt ttatttgata gtatggttac
     4081 ttttccactc ttaccattag cagtaatttt tctaagtatt ccaacttctt ctactatgcc
     4141 agtaaacatc ctaacctcct atatgaatgt attaacaaaa atttaattta aaacatctga
     4201 ttctataaga atatcgccat ctatcaaagt tacattattg attgttaact gtgttgaatc
     4261 tgacaaatga tttattcctt ctccagaaac gaatgtaggt gcatctttac cacctataat
     4321 cttgggtgat agaaataatt tgactttatc aactaactta tttctaaaga gactgtcatt
     4381 gagagtagca ccaccttcaa ctagaacaga atcaatgttt aactctccta atttatacat
     4441 tacaaattcc aaatctacta agttgttttt tgaaggagaa attattgttt caacattatt
     4501 ttcttttaac atagataatt ttatactttc tttgcaacat gtgaaaacta tagttttact
     4561 ggttttatct ttaaccaaat tactattaag tggcaacttt aggtgtgtat ctattactat
     4621 tcttgtagga tgagacactt tttcttgatt gagcctgcaa gttagtaaag gattatcgtt
     4681 caaaactgta tttatgccaa ccattatact ttggtattta tttctgtatt tatgagttag
     4741 ataccttgat gattcattag atatccattt tgattctagt gattttgtag caattttacc
     4801 atctagactt actgctgatt ttacaataca aagaggtctt ttgtttttta tatgataaaa
     4861 aaatgcctca tttaaatctc ttgcttcatc ttctaatatt cccacagtaa catcaatatt
     4921 gttatctttt aattttttta ctccattacc acaaacaagt ggatttgggt ctaatgtaga
     4981 tattacaact ctcttaatct tattttgtat aattttatct acacaaggag gcgttttccc
     5041 ataatgagaa catggttcta agtttacata aagagtagag tcttttagac tttgtttagc
     5101 actgtttata gcatttactt cagcatgatt agagccaaat ttctcatgat atcctttgcc
     5161 tattatatta ctatctttaa caattacaca cccaactaat ggatttggat ttacaaagcc
     5221 ttctccattt ttagccaatt caatagcttt tttcatataa taaatatctt tttctttttg
     5281 attcacaata tgtacacctc ctgtttaatt ggataatttt taaacaaaaa accctgagaa
     5341 ttaactcagg gtttagaata atctacaata aatgtttaat aaaagtagaa gaaaattgct
     5401 ctacaaaaac actcattaaa tatcttcttc catccagact ttactgtcgg ctttggaatt
     5461 taaccaaatc aaccaagttt taccttggct cgcgggctat accgccgatt gggaatttca
     5521 ccctaccccg aagattaagt attaaattta tatttagaat gtatcataaa ttattttact
     5581 ttgcaaacta tttttttaaa aaatatatgt taatatataa tatagaatgt ctataaactt
     5641 tgaaatacca cgattgtgaa tataaaaata aaaatttgaa cataatcagg aaagaagtct
     5701 aattaattta aattaggaga gtaatatatg aataaaaaac ttttagaaat actatcaaaa
     5761 tactatggat atacaagttt tagaaaaggt caggaatcta taataaattc tatcttaagc
     5821 gaaaaagatg tgttagctat tatgccaaca ggagggggga aatcaatatg ttatcaaatt
     5881 ccagctttga ttttagatgg aatgacaata gtaatttctc cacttatatc acttatgaaa
     5941 gaccaagtag atgccttaaa gactatggga attagtgctg cttttataaa tagttcatta
     6001 agcagcaaag aatttaatga aatattaaat aatattagaa aaaataatta taaaatactc
     6061 tatattgctc cagagagatt agatgcacaa gagtttattg agctaataaa taataacaat
     6121 gtaagtcaag ttgcgataga tgaagcacat tgtgtatctc aatgggggca tgattttagg
     6181 ttgagttata gaaggatttc tgattttata aaaaatttgc ctaaaagacc tatagttaca
     6241 gcatttactg caacagcttc agaagaggta agaactgata taataaattt actgtgttta
     6301 gaaaatccag attattatat aactggattt gatagagaga atttatcaat aaatatagta
     6361 aaatcatcta gtaaaaataa gtatatttta gattatattc aaaatcataa aaatgagtct
     6421 ggaattatat atgttgcaac tagaaaggaa gttgaaaata tatataatgg tttatctaag
     6481 agaaatatat ctgtatcaaa atatcatgca ggtttatcta aaaatgaaag aagtaaaaat
     6541 caagaagatt ttataaatga taatgtggat ataatggtag caacaaatgc atttggaatg
     6601 ggtattgata aacctaatat aagatgggtt atacattaca atatgcctca aagtatagaa
     6661 aactactatc aagaaatagg tagagctggt agggatggag aaaaaagtga gtgtgtactt
     6721 ttattttctc caggagatgt acatatacaa aaatatttaa ttgatattgg agttgaaaat
     6781 cctgaaagaa aattatttca gcataagaag cttcaataca tgattgactt agtttacagt
     6841 aattcatgct atagaaagac aatattgagt tattttggag aaaattatat agacaattgc
     6901 aataattgta gcaattgttt agtagagggt gaaatagtag ataaaactat agatgcacaa
     6961 aaagtcattt catgtgtagc tagaatgaaa agaagttatg gtgttactac tattgtagat
     7021 gtacttagag gttctaagaa taaaaagatt ttacagcttg gattaaatac tttgtctaca
     7081 tataacatta tgagagatta ttctagtgaa gacttaaaga attttgtaaa tacattggtt
     7141 tctcatggtt ttttagattt agttgagact ctaggcaatg gaaatagagg tagttttcct
     7201 actataagat taaatgagat gtctatgaaa gttttaaaag gtgaagtgaa agtagagttt
     7261 aaagaaattg ttatgacaaa atcattggaa gtagaggatg agttatataa tgctttaaga
     7321 gaacttagac attcaattgc aagtgaagaa aggatagctc catatatggt atttggagat
     7381 ggtacactta gagccatgtc tagtagttat ccaattaata aagaagaaat gctagatatc
     7441 tctggagtag gagaaattaa atatcaaaaa tatggtagag attttgaagc agtaattaaa
     7501 gaatatgtcg aaaaaaataa tataaataaa aaaatagatg aaaaaaatgt cgatttaaag
     7561 aacgttaata gtgatttctt agaggttaat acagataaag ttttatatga aaaactgtta
     7621 gtcattagag aagaatatgc aacaaaggaa aaattacctg catatatgat tttagccaat
     7681 aaggctttga aagaaataag tggaagatat ccacttgatg aagaacaatt aaaagatatt
     7741 tctggcatag gtggagtaaa atttgaaaaa tatggagcta atattttggc agaggttaat
     7801 aaatatgtaa aagaaaataa tattaatgtt atttgggaga aaaagggcaa aaggaagtta
     7861 atattagatg gagaaagtag aaaaaataat gaaatagcat tagatttgct aaatcaagga
     7921 aaagatatac aaagtatcag tcaagaaata gaagtttcat tgtcaaccat tattggatat
     7981 gtgcatgact atgtaaagga tggacatagt atatcatttg aattaggact agaaaatttt
     8041 tataatgaag ataaaaagaa gatgatttta gaagtttgcg aaaaagttgg ctatgataat
     8101 ttaaataaca taaaaagaaa acttccagat tcaataaagt atgaaaatat aagagctgtg
     8161 atgcttgact cttatcttga aaacttaaaa aataatatta tagcagagta atttttaact
     8221 taataaaaat aaaatttgta ttattgtgtt gaactaataa ttttgctgtg ttatactgaa
     8281 tttgtaaaaa taatgaaaaa ttagagctag gggcgctaga attggctagc tgagagataa
     8341 agccgtaact tacaaaccct aataacctga tcaagataat gcttgcgaag ggaagctttt
     8401 taatataatt ttattatata aataggcaaa ttattcaatg ttaggagaaa tttgtctaag
     8461 tgtatatacg attataagag tgctttccaa acgaggaaaa gcactttttt tattttcaaa
     8521 aactataact ttaatcttgg aggattgtaa tatgaactat acaacacaaa tggatgctgc
     8581 tagaaaaggc ataataacta aagaaatgga aatcgtttct caaaaagaac aggtagatgt
     8641 aaatgaatta agagaattaa tagcaaatgg acaagttgtt ataccagcta ataaaaatca
     8701 taaatcttta agtgcagaag gtgtaggtaa aaatttaaga acaaaaataa atgtaaatct
     8761 aggaatttca agagattgta aggatataga aaaagaactt gaaaaagtta gagtagcaat
     8821 agacatgaaa gctgaagcta taatggattt aagtaattat ggcaaaacta gagaatttag
     8881 agaaaaggtt gttgaaatgt cacctgcaat gataggttca gtacctatgt atgatgcagt
     8941 aggatatttg gaaaaagaat taaaagatat aacagaagaa gagtttttaa atgtaataag
     9001 acaacatgct attgatggag tagactttat tactatacat gcaggtctta caagaagtgt
     9061 ttgccaaaaa ataaaaaatc atgagagatt gactcatata gtatctcgtg gaggttcttt
     9121 attgtttgca tggatggaat taaataataa ggaaaatcct atttatacta attttgataa
     9181 aatattggat atatgtgaag aatatgatgt tacattgagt ttaggagatg cttgtagacc
     9241 aggttgtata aaagattcta ctgatggtgt tcaaattcaa gaacttgtag tattaggtga
     9301 acttactaaa agagcatggg aaagaaatgt acaagtaatg atagaaggtc ctggacacat
     9361 ggctattgat gaaatagaag caaatgtagt attagaaaag agattatgcc atggagcacc
     9421 tttttatgtt cttggaccat tggttacaga tatagcacca ggttatgacc atataacaag
     9481 tgcaatagga ggagctttag catgtgctaa aggagtagat tttttatgtt atgtaactcc
     9541 agcagaacat ttaagattgc ctaatttaga tgatatgaaa gagggtataa tagctgcaaa
     9601 gatagctgct catgcaggag atatagctaa aaatgttaaa ggtgctcgtg aatgggataa
     9661 taagatgagt aaagctagag ctgatttaga ctggtgtgag atgtttagac ttgcaataga
     9721 ccctgaaaaa gctaagagat acagagatga atcaactcct actcatgagg atagttgtac
     9781 tatgtgtgga aaaatgtgct caatgagaac agttaagaaa atattaaaca atgaggaact
     9841 taatctaata taatttttgg ctagtatgac tggaataaaa agaagctgaa aagaggaggt
     9901 gatttagtga aggttaatgg gaaggaaatt gaatttgaaa aagatttgac tgttatagat
     9961 ttattaaata aatataattt aaaaagtgat agagttgtag ttgaagtgaa tttagagata
    10021 attgaagaat ctaactacaa tacatatgta ttaaaagacg aggacattgt agagcttatt
    10081 agctttattg gaggtggtta aaataaaaat atttgtaaat gaattattaa cagatgttga
    10141 ggaaaataca actgcttata aagttagaga tagctataac aataaatgtg atgtattggt
    10201 gttaaatgga tatccaatta aaacagatat gcccctttgt gaaaatgata aattaacact
    10261 tatacaaaaa ggagttaagc catcacttga tgaattggaa agcttgttga ttgctagaca
    10321 tactccaaat atacataata aattaaaaaa agggaaagtt gctatattgg gattgggggg
    10381 acttggttcc aatatagcta tctctttagc tagaatagga gtgggagaac ttttacttat
    10441 agattatgat gttgtagaac catctaattt aaatagacaa cagtatttta tagatgatat
    10501 aggaaaacta aaaactgatg ccatgataga aaatataaaa aaaataaatc catttataaa
    10561 attaaataaa cgagatattt ttttaaacaa gaacaatatg gatactatta aggattcaga
    10621 tttaataatt gaggcctttg atgatgcttc atgtaaagca caggtatgta attatgtatt
    10681 aataaattta aaagataaat atttaatagc atcatctggt atggcaggtt actatgattc
    10741 aaatataata actactaaga agataaaaga caggttttat atatgtgggg attttgtgaa
    10801 tgaagctaaa tttggtgaag ggcttatggc tccaagggtt gccatctgcg caaatcatat
    10861 ggcaaatttg gcaacgcaaa tacttataga tatgtaaata ttaaaattag atataaaaaa
    10921 gattgaatat taaaaaaatt taacgtggag ggaaataata tggataaatt ggtacttggt
    10981 ggacatgaat ttaatagcag attattggtt ggaacaggta aatatggttc aaataatata
    11041 ttgcctgagg taataaaaga aagtggaagt gaaataataa caatggcact tagaagagtg
    11101 gatttagaca ataaacaaga aaacatatta acgtatattc caaaagagat gacaatactt
    11161 ccaaatacat caggagctac taatgcagaa gaagctgtaa gaatagctag aatatctaga
    11221 aaaatgggat gtggagattt tataaaaata gaagtaatat cagatactag atatttactt
    11281 cctgataatg aagaaactat aaaagctaca aagatacttg ctgatgaggg gtttatagtt
    11341 cttccatata tgacaccaga cctttatgct ggaagaagac ttatagaagc taatgcagcc
    11401 gcagttatgc cacttggagc tccaattggt tcaaatagag gtcttcaaat gaaagaaatg
    11461 ataagaataa tgatagatga gttggatata ccaataatag tagatgcagg tattggaaaa
    11521 ccatctcaag ctatggaggc tatggaaatg ggagctgatg cagtacttgt caacactgct
    11581 atagcttcag caggagaccc tgtacaaatg gctagagcat ttaaattagc tgtagaaggt
    11641 ggaagagaag catatattgc aaaaacagga aatgtgtcag aatttgcaaa tgcatcttct
    11701 cctttaacag gttttttagg caatttatag aaagtggtga ttggtgatga gtttttatga
    11761 tgtaatagaa aaatataggg attttgattt tgatggatat ttaaataatg ttactgataa
    11821 tgatgtttta agaagtttat caaaagacaa gcttgaagat tttgatatat taaatttgct
    11881 ttctaaaact gcagtaaaac atttagaaga tatggcacaa aaagcacata aattaagtgt
    11941 tcaatacttt ggtaaaacag tatgtttata tacaccaatg tatatagcaa attattgtgt
    12001 taatcaatgt gtttattgta gctataatat aaagagtgga ataaaaagaa aaaaattaac
    12061 tatggatgaa attagagaag agggagaagc aatttcaaaa gaaggtttca aacatctact
    12121 tgttttgact ggagaaagta gttttcactc aagtgtagaa tatataggtg aggctataga
    12181 aatattaaga gagaaatttc cctctatagg tatagaagtt tatccaatgg aagtagaaga
    12241 atataagtac attgtagata aaggtgttga aggattaaca gtatatcaag aaacttatga
    12301 cgaggaaata tataagagag tacatataaa aggcccaaaa tcaaattatg aatttagact
    12361 agatgctcct gaaagagggg caaaagcagg tatgaggact ttatcaatag gtgcactttt
    12421 aggtctaaat gattttagaa aagaaacttt ttttacaata ttgcatggga aatacttaaa
    12481 aacgaaatat cctcatattg agctttcata ttcaacacca agaatgagac cttttaaagg
    12541 gtgttttgag gaattggtag atataagtga tactgattta gttcaagcta tggtatgtat
    12601 gagattattt gaccctcatg cagctataaa tatatccact agagaaaatt tagaaatgag
    12661 aagtcatata atacctttag gagtaactaa attgagcgca ggtgtatcta ctgatgtagg
    12721 aggtcattct caagatgaac atgatactgc tcaatttaag ataaatgatg aaagtactgt
    12781 taaagatgtg gagaaaatgc taaactcaat tggttatcag catgtgttta aagattggga
    12841 aagattttaa atgtatctga taacaaacag aaaattatgt agtgaggaac gatatttaga
    12901 agttattaaa gagtctatat tgagtggtgt agaaaatata ataataagag aaaaggattt
    12961 ggaatatcaa gagctaagaa aattatatat gaaaataaaa actaaaatca attgtataga
    13021 ttttcaagag caaatatcag atgaaagttt gaaaactaat ataaatcaaa aagagtgtag
    13081 aaataaattc aaggttaatt ttataataaa tagtaacatt gaattttttg aaaaagtgga
    13141 ttgtcaaggt atccatcttc cttttaaatt attcttgaat ttaattgaaa ataagtataa
    13201 ttttaatgaa aataaaatac taggattatc tttacataaa gtagaggaag tagattattt
    13261 agaaaaatta ataagaaatc aaaatataaa aatagattac ataacattat cacatatata
    13321 tgaaactaaa tgtaaagaag gattaaatcc aaaaggaatt gagcttttaa aagaagctaa
    13381 aaagattact gatat
//
LOCUS       DAEPLF010000084        13379 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEPLF010000084 DAEPLF010000000
VERSION     DAEPLF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13379
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.084"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(179..922)
                     /locus_tag="KR314_002095"
     CDS             complement(179..922)
                     /locus_tag="KR314_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBH0328478.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            1088..3655
                     /locus_tag="KR314_002096"
     CDS             1088..3655
                     /locus_tag="KR314_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0328479.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNNSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAVDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            3885..4289
                     /locus_tag="KR314_002097"
     CDS             3885..4289
                     /locus_tag="KR314_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0328480.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            4313..4810
                     /locus_tag="KR314_002098"
     CDS             4313..4810
                     /locus_tag="KR314_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0328481.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            4813..5214
                     /locus_tag="KR314_002099"
     CDS             4813..5214
                     /locus_tag="KR314_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBH0328482.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            5232..5720
                     /locus_tag="KR314_002100"
     CDS             5232..5720
                     /locus_tag="KR314_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0328483.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            5747..6502
                     /locus_tag="KR314_002101"
     CDS             5747..6502
                     /locus_tag="KR314_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0328484.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            6502..7365
                     /locus_tag="KR314_002102"
     CDS             6502..7365
                     /locus_tag="KR314_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0328485.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKKKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            7412..7891
                     /locus_tag="KR314_002103"
     CDS             7412..7891
                     /locus_tag="KR314_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBH0328486.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            7956..9263
                     /locus_tag="KR314_002104"
     CDS             7956..9263
                     /locus_tag="KR314_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0328487.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETDMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            9574..9714
                     /locus_tag="KR314_002105"
     CDS             9574..9714
                     /locus_tag="KR314_002105"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBH0328488.1"
                     /translation="MNGEGCILSNKITKECYKTGYMIREKSIDTYPDSGRGFMTWDEN
                     GE"
     gene            10226..11320
                     /locus_tag="KR314_002106"
     CDS             10226..11320
                     /locus_tag="KR314_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0328489.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQTQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSLLSENKEYKFLIEKLSLLEE"
     gene            11325..11525
                     /locus_tag="KR314_002107"
     CDS             11325..11525
                     /locus_tag="KR314_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBH0328490.1"
                     /translation="MENLMEYLPLLIPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            11525..12442
                     /locus_tag="KR314_002108"
     CDS             11525..12442
                     /locus_tag="KR314_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328491.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLEPDSGTIDVCGEKVTYGRTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIKIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNNEIQKFKSSGGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            12439..13197
                     /locus_tag="KR314_002109"
     CDS             12439..13197
                     /locus_tag="KR314_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0328492.1"
                     /translation="MREYIAFTKKEFKENIRNYKLLSLTILFLVFGIMSPLSAKFMPD
                     LIAHFAPTLKVTTAPTALDSWTQFSGNISGLGTSLTLIVFCNILSNEYSKGTLVIMLT
                     KGLSRSSVVLSKFSIAVIIMTIGFWLSFLCTYGYTMYFWPTANLNHIIFSAFNLWLIS
                     IMYISILILGCVLFRPAFASVLLVLAATAILSLISIPKQIAPYTPNFILSKNIDLVSG
                     KVTPPEFIIPIIVTIVISIVCLLLAVVLFNKKAV"
BASE COUNT         5129 a         1413 c         2470 g         4367 t
ORIGIN      
        1 aactaagcct attaaaaatg ttataatttc tattaccatc atagaccata tcaatccatc
       61 aagttcataa aagtaattaa gctttattaa atagcttctt tattataatt tctttatttc
      121 atataatata aatagtatta aaatttttat gggagttaaa gtaagcatgt attattactt
      181 agatatttgt ataatcaaca tctagtaatt tacctttttg gtgaaaatga ttaataagtt
      241 ttttaggata attcattatt aattcatctg gaaaattaat ttcctttaag atattctcag
      301 aatactcata gttacctatg tcaaaacaaa tgtgagcatc tgaagttagt atgattttag
      361 caccatattt tttgcaaagt gtagcaatat acttacagtt atccgagcta ccatttcttg
      421 acacgccttt aatagaacaa ttatttattt ctattaagat attcttttct atagctaatt
      481 taatgattaa ttcatagtcg attgggtagt ttgggtttcc aaggtgtcct aatatttcaa
      541 tattatcatg cttatttatt gcatttatta aagctttagt attattctct aatgtatttg
      601 gagaaaaaac agcttcgtga aatgacaaga taagataatc taaccgagga attacaaaag
      661 gctctaggtc tatgtttcct ttaatatcta gtatatttgc ttcacagcct cttaaaatta
      721 taatgttatt tatgattctt gggatatttt gaatgttgtg aaagtaccat ctgtggggag
      781 aaccaggcat cttaggacca tgttcagatg tacctagtat cttaattcca ttttctttac
      841 aataattaat attttctagt aaagtgctat agccatgacc actgactata gagtgggtgt
      901 gtaaatctga taaaaaattc atataaattc ctcctctata aaagatgagg gtaacgtaat
      961 ttatattata aaacgaatag gaaagaaaat caatagttac ataattagac aaaaatatta
     1021 aaatattgta gaattaagct taataataaa tgtttaacag tattactgct tataggggag
     1081 gttaactatg acacaaaaga agctatcaag gaaagaaaaa gttttaattt gcttgaaaag
     1141 gttaacagaa gaacacttga actttaataa tggcattaga tttggatttg atgcagaata
     1201 tatagctact gaagtaggta taaatagaag taatgctagt aaggaattaa atcaattaat
     1261 aaaagaaagt attattataa agataaaggg taagcctgtt cagtacttat ataaagaaag
     1321 tattgagagt attttgggaa agaaactaaa taaagggata tttgataata ttaactctat
     1381 aaacgaatat agtgaaaatg attctatcaa agatgagtat attttagaga gtataaaaaa
     1441 agagaacaat atttttaatt tagtaggatg tgatggaagt ttaaggtccc aaatagaaca
     1501 ggctaaagca gcagtaatat atccacctaa ggggttgagt acattaataa taggacctac
     1561 aggagttggt aaatctatat ttgcagaata tatgtataag tatagtttaa ctataaaaga
     1621 agatagtgat aatgcaccat ttatcatatt taactgtgct gattattctg ataatcagca
     1681 attattatta gcgcagcttt ttggatatgt aaaaggagca tttactggag ctgataaaga
     1741 taagtatggt attgtacatg aagctaataa tggagtattg tttttagatg aagttcatag
     1801 attatctgca gagggacaag aaatgctatt tctgcttatg gataaaggga tatatagaag
     1861 gttaggagag gctaataagg tacacgagtg tagagttatg ataattgcag ccactacaga
     1921 agacccagaa acagcaatgt tggaaacgtt tttaagaaga atacctgtaa ctataaagat
     1981 tccgtcttta gaggaaagag gctttcaaga gagaatgaag cttatatgtt atttttttaa
     2041 agaagagagt gttaggattg gtgttaagtt aaaggtttca aaagaagtta ttaaggcatt
     2101 tattttatat aagtgtaagg gaaacatagg ccaattaaaa agcgatatac aattaatttg
     2161 tgcaagagct tttttagatt atatgaccta taaaagggaa tgtgtatatg taaggctctc
     2221 tcttctacca aataatatta gagaatcatt atatggaaat aatagaaaag aggagtttgt
     2281 acaaaatttc agttttattg gaaaagatga tattattttt ttaccagaag aaaaggattt
     2341 tgaaagtgaa aatttattaa tagaaaataa aaaatatgat ttagattttt atggaatgat
     2401 aaaagaaact tggaataaac ttcaacaaga aggtattaaa gagtcacaaa ttagaagtgt
     2461 tatagatgga gacatacaga agtattccta taatttaatg aatactttta tatataataa
     2521 tagtaatcaa acagcttata acaacattgt taatgattca attgcaacta ctgtaaaata
     2581 tattttacaa aagcatgata aatggagcaa aagagaagga ttagataaat taataaaagc
     2641 tatagcctta catattcaga atttaattga aagaataaag attggaaata tagtaaagca
     2701 tcctaataaa gatgaaatta tgaaggaaag agcatatgaa tataacatag caaaagaaat
     2761 attatctaac atgtcattaa tgtatggagt agagtttcca gaagatgaag caattttttt
     2821 agcaacattt ctttatttat cgtatgtggg attaaatgag gagagtatag caatattagt
     2881 cataatgcat ggtgaatcta cagcatctag catggtgaat gtagccaata ctttactgga
     2941 ttgtaaccat gcagtggcaa ttaatatggg attagaagat agagttcagg atattttagt
     3001 acaagctgta gaaatagcca ataaaattga taaaggaaag ggaattttaa ttttaactga
     3061 tatggggtct attttaacat ttgctaaagt tattcgtgat gctacaggaa aagaagtaaa
     3121 agcagtagat atggtaagta cacctattgt aatagaagca actagaaagg ctctaactcc
     3181 agaaatgaca ttagagaaat tatattttaa tattatagaa tctataaaga aacattatgg
     3241 agatatggaa gttacatatg ctgaaaaaga taatggtggt agatattttg atagactact
     3301 aattgataat attagtaaaa ctttgacttt tttaaatggg gaaaaggctt attttatatt
     3361 aaaagaagta attaatagaa ttagtgaaca ctattcttta gtaattcaag atgaactttt
     3421 agtaaagttt atatttcatt gttcatgtat gattgaaaga gtaataataa aagagtcttt
     3481 agtttataaa tcatatgaag aaagaattag tagaaataaa gaattatatt tagttataaa
     3541 agaaagtttt aagctagtag aagagacatt cgatataatt atttcagata tggaatatgc
     3601 aaatatatta gatatatttg aatcacaata tgatacagat aaattcaaaa gttgatacaa
     3661 aatttaaaaa tagccaaaag gctatctttt ttttactttt tttgatacag atttgataca
     3721 ggttgaaaaa atcttttgat acatatcgaa ataataagtt tttaaaaaaa attgagtaaa
     3781 aagttttgat taaatcctgt aaaatagcca tttattattg atacacataa gttggcacgg
     3841 caattgctta tataaaaagt gacaagaaaa gtgaggtgaa attggtgata ggaataatag
     3901 ttatgactca tgggagtttt agtgaagaaa ttataaaaag ctgtgaactt attgcaggac
     3961 cagcagaaag aacagcagca attaaactaa ataggaatga taatattgaa gacttaaata
     4021 aaaattttgt agaaaagtta aatgaattag atgaaggtga cggagtttta gtattagctg
     4081 acctattagg aggttcgcca agtaatgttg caagtttaaa ccttaaaaaa ggtggcaagt
     4141 ttcatgcact tactggagta aatcttccaa tgctacttga agctttaatt aatagagagg
     4201 gaaaaagttt agaagagtta gcagaagcat gtattgaagc aggacaaact ggtataaaca
     4261 atattaataa agttcttagc tcaatgtaat agaaaaagga gtttgattaa aaatgtcttt
     4321 agtaaaatat tgcagatgtg atgatagatt aatacatggt caagtgatat ataaatgggt
     4381 taagcatcta ggtgtaaaaa agatagtagt agtagatgat gaaacaacca atgatgttat
     4441 tgctaaaggt ttgataaaaa tggcagcacc aaagaatatt gatttatcaa tattaactgt
     4501 aagtgaatca aggagatatt tttataataa tcaagctgat gataacgttt ttgtattaat
     4561 taaaaattta gatactgcaa atagaatgat agaagaaggg attaatatca aaaaacttat
     4621 tgttggaaga atacctacag gaataggaaa aaagaaaata tctcaaaatg tatacattaa
     4681 taaaaaagag tttcttttaa tagatgaatt tattaaaaag aatattaatg tatcaattca
     4741 aatggtacct gatgaagaag aagtagaatt aaaccaaaat attaatcgct ataaaggaga
     4801 gtttatttag caatgaataa atataaggta attatactgt gccatggaac ttggggaaaa
     4861 gtcttagtag aagaaacaaa taaaaacttt ggattgtcaa atcaatatga agttttatca
     4921 ctaagttctg aaaaagatgt ttctgtattt atgaaagagg ttgaagaagt agtagataaa
     4981 gaagatgtga aaattataat atcagattta tatggaggat ctacatcaag tgttgcaata
     5041 gctgttgcta taagaagagg aatagatgca ataaatggac ttagcttaca gacaatttta
     5101 atcgtagatg aagaattaag caaagaagat aaattacata cattaccaga aagaatcgtc
     5161 aaaaaaaata ataacctttg tgtggattta attaaagaat ttaaaaaaat ataaattatg
     5221 aggtgaatat aatggcaaaa ataacattag taagagtaga ccacagatta attcatgggc
     5281 aagttataac aaaatgggta aaaatcgctc aagctcaaaa aataataata gttgatgact
     5341 tcttaggtca agatgaattt atggctgaca tatataaaat ggcagcacca tcaggagtag
     5401 aagtagttat tttaacagca gaagatgcgg gacaagcatt ccaaaacaac actttaggag
     5461 ataaaaatat atttattcta tttaaaaatg ttgatatggc aaataaggct tataaagctg
     5521 gacttaaata tgaaaagatt caattaggag gtattccaaa tgaagcagga aagaaaatgg
     5581 tgtttacagc tgtttcatta ggaaatgagg atgtagaaca acttaatgaa cttaatgaaa
     5641 atggggttga aatagtccta caagttatac cagaggaatc atctatgaca tatgaaaatg
     5701 cgttaaagaa atttaaataa aaaatataaa ttaggaggaa ttataaatgg aatctgtttt
     5761 gatgttggca attgtaacag gattatggta ttggtttgca gcaggtcttg cagggtatac
     5821 ccttttctca acactaaagt ctcctttatt tataggtttt agccttggat tactttgggg
     5881 tgatgtaaca actggtatga ttgttggtgc aagtatagaa atggtttatc taggaatggt
     5941 cgcagcaggg ggaaatattc catcagataa atgtctagct gctcttattg caataccagt
     6001 agcacttcaa actggtgtta atgcagaagt tgcagtatct attgcggtac ctttaggtgt
     6061 tataggagta ttagttaata acttaagaag aactggtaat gctgtattgg ttcataaggc
     6121 agataagtat gctgaagaag gtaatacaaa aggcatctgg agatgtgcaa cactttattc
     6181 tttaatattt gggtttgttt taagatttcc aatagttttt gtatgtaact tttttggagc
     6241 agatttagtt caatcattat tagatgttat accacaatgg ttaatgaatg gattaacagt
     6301 aatgggcggt atattacctg cattaggttt tgcaacaaca atatttacta taggtaaaaa
     6361 taaattctta ccaatgttta ttataggatt ttttatggtt caatattttg aaatatcaat
     6421 tactgcagct gcaatatttg gtgtttgtat agctctttta attaccttta tgaaagaaga
     6481 caaaagggta ggtgaagtat aatgcaagaa ataaaaaata aagaagttaa aaatggacaa
     6541 ggtccaactg aagttaataa gacaagagta ctcacaaaga aagatgtaac aaaaacatac
     6601 ctaagatggt ggtggacagc agagttatct aattcttttg aaagaatgca agcattagca
     6661 gtttgtgcaa gtttcactcc ggctttagaa aagttatata agaaaaaaga agatttagta
     6721 gatgcattaa agagacactt acaatttttt aatactcaag ctatatgggg aggattaatt
     6781 catggtacag ttttagctat ggaagaagag aaagctactg aaggaaaaat cccaggagaa
     6841 gttatttcag gtgttaagaa tggtcttatg ggacctttgg ctggaattgg agatacttta
     6901 gattttggta catttcaaac aatattctta gctttaggtg cttcatttgg agcagaagga
     6961 agtgttatag gtgcattttt cccaataatg tttagtatat tactattttg cgaaggttat
     7021 tacttatttc atttaggata ttctttaggt agagattcaa ttaagaaaat tctatcaggt
     7081 ggtattgtca ataagataat tgatggtgct tcaattctag gaatgtttat gatgggagca
     7141 ttatctgcaa ctacagttaa gctttcaact ccgctatcat ttgatattgg tggtaaagca
     7201 attgttgttc aagatacatt aaatatgatt gctccaggtt tattaccact tggtgttgta
     7261 ttcttcgtat attggggaat gaaatacaag aagtggacaa taactaagtt attagtaatt
     7321 ttagttgtat tagcattggt aggatcattc attggaatat tctaattcac agtgattaaa
     7381 atatagaatt taattttagg aggagaaaga aatgtcagaa aaaatgtata cagaaaagga
     7441 atatttagaa ggaattagag gagcaacagg agaaagagca gtatggttct atcttcttat
     7501 gaaagaagct gaaaaattag gagctaatcc agatgatata tgtaaagaag caatatatgg
     7561 atttggtaaa atgagaggtc aaaaatataa cgtagcagat actccaggaa aaatggcaga
     7621 aatgttatat aacagtaaag gacaaaaagt gtttgaaatg gaattagtag aaaatacaga
     7681 tgagaatggc gttttaaaat tccatcactg tccattagat gctgcttgga aagaatatgg
     7741 attaactaaa gaagaaagaa aagaaatttg tagattagct tgttatgggg attatggaag
     7801 agttgactgt gcacaaggtg ttaaattaga ttttgctcaa aaatgtgctc atgatgatga
     7861 agtttgtgaa ttagtattta caaaaaaata atttttaatg ctaaaagcct gttctttaag
     7921 gacaggcttt aaaaagatag aaagtaggtt gatttatgga tataagcaga agtgtagata
     7981 atagtttaaa tagaaccata gaatttctaa aagaattgat aaaaattgat tcacaacaag
     8041 gtgaacctat ttcacaatgt ccttttggta taggacctaa aaaatcttta gataaaactt
     8101 tagattactg tgctagttta ggttttagtg ttaaaaatat tgataattat attggatatg
     8161 cagaaattgg agagggagag gaattaatag ggattccaat gcatttagat atagttccac
     8221 caggagaggg gtggagtgta gacccatttt cgggagcagt aattgacaat attatttatg
     8281 gaagaggtgt catagataat aagggtgctg tatctatgct tatacatgta ttgaaaaaca
     8341 tagaggatat gtatcctaca attaataaaa gaataagatt aatttttggt acaaatgagg
     8401 agacagatat gaaatgtatc aaatattact tagataaagg tgaagaaatt ccaagtatgg
     8461 gatttactcc agatgctatg tatccagtgg ttaatggaga aaaaggaagg gttcatataa
     8521 ggatagaaaa agaaattaaa attgacaaaa gtaaacctta cataatagta aggggaggaa
     8581 ctaaagaaaa tgttgttcca tctcattgta ctgcaaaaat tattaatgga attatttcag
     8641 agttaactac taaaggagta gctgtacatg caagtaatcc tgaaaaggga gaaaatgcta
     8701 tatctaaaat ggtaataaag atagttgagg ataatatgga ttttcagcat agagaagata
     8761 tagaattagt ttcaaaatac ctttgtagtg attattatgg agatgcattg gggattaatc
     8821 aatatgatga ggtattcaaa aacactacct taaatttagg aatattaaaa gtaaatgagg
     8881 aaaaaatcgt atgtgagtta gatataaggt atggtaaaaa tatagtttta aataacataa
     8941 tagatagatt caaaaaggtt ttttgtaatg gttggaaaat agaggttatt gctcataaag
     9001 acttacatta tgtggatgag agtaatttag tacttaaaaa attattagaa gcctatgaag
     9061 aagtaacaga tgaaaatgga tatacaattg ctatgggtgg tggcacatat gcaagttggt
     9121 ttaaagatat ggtggcgttt ggacctaagt tcttagcgta taaaactggt ggtcatggag
     9181 tagatgaaag agtaccaata aatcatatac gaaagaatat ggagatatat actttggctt
     9241 taataaaact attagaattg taaaagaaga agagacttta tgtgcaaatt tacagatatc
     9301 ttaaaaagag agataaaacg agttcaactt tatttaaatg ttcaacacca tcaaatgggg
     9361 ctgtctcaaa atagagataa gcctcttgct tattattgaa atgatgaaaa tatattttac
     9421 ttcatacaaa aaagagtgcc ttatgaagta aaaagttaat tttgagacac cctcatttga
     9481 tgatgttgaa gagaaaataa gtatgaaatt attaaaaaaa gatgatttta taaaagttaa
     9541 ggttgaaaat aaaagttaat tgaatggaat tagataaatg gagaaggctg tatactatct
     9601 aataaaataa caaaagagtg ttataaaact ggttatatga ttagagaaaa gtctattgat
     9661 acttatccag atagtggacg gggatttatg acttgggatg aaaatggaga ataataaaaa
     9721 ttatagttta tacaatttaa taagatatat gtacaagcag ttaatctaaa tgattgactg
     9781 cttttctttt atagattttt atataataga caattactaa tacatgatag ttgcctgtta
     9841 taattagagg aatagatacg aataataact ttataaaaga attaaatgga aaattgaaga
     9901 gttatattca ggattttaaa aagaatatga aatgagaaat gaaaataagg tgtttatatt
     9961 aaaagaggtt ttacacaaaa tacttaaaaa cgtgataaat aaaaactgta tttagataaa
    10021 ttaactacta gttttatata taatatggat tttgtttaca aagtttacat atttcacaaa
    10081 atatttttaa aatatattta aaaatatatt ttatatactt gttcgtttgt ttctctgtgg
    10141 ttgatttttt cgtcaaaagt caacttcaaa tcgcttgtta gctttacatg tttaaggata
    10201 taattataca taaggaggga ttgacatgaa tgaattaaac atcgcaaaaa cgctgatttt
    10261 aaagcgtaaa gaaaaaggaa ttacacaaga tgaacttgca aattatatag gcgtttctaa
    10321 agcttctgta tctaaatggg aaactggaca aagctatcct gacataacct ttttaccgca
    10381 actcgcgaca tatttcaaca ttaccgttga tgagttaata tgttatgaac ctcaaatgat
    10441 gaaggaagat attaataaac tgtataataa actgtgcaaa gactttacgg ttaaaccgtt
    10501 tgatgaagtc atggatgaaa taagagaaat cataaaaaaa tattattcat gttttccttt
    10561 gatttttcgg atgggtcttt taatagttaa tcactatgat attgtagatg aaaaaaagcg
    10621 tgaactatta attagtgaag cattagaaat atttattcgt atacaggaaa cttgtaatga
    10681 catagatatt tgccgtcaag caaaaagtat ggaggcaact tgttatatat tgttaaatca
    10741 acccatacaa gtgattgatt tattgcaaaa cagcaatttt cccatgataa atgaatctat
    10801 acttcttgca caggggcaaa tgatgaatgg tcaaatggat gaagcaagag aaacatttca
    10861 acttggtgca tatcagaacc ttatatcatt agtccaaaat ctagtgggta ttttgcaaaa
    10921 tgcagataaa ttacaaatga aggaaataga aagacgtatt ctagcaattt cagatatatt
    10981 tgagcttgat acattatctc ctgctattat gctttcttca tatttaacac agacacaaat
    11041 taatttaata catggggata atgaaggagc tattaaatca ttacgaaaat atgtagattt
    11101 agcaacacgt gatatatacc caattataat acatggggat gactttttta ataaacttga
    11161 cagatggatt tctgaaattg gaacaggtat tacacgtgat gatacaatcg taaaagcagg
    11221 tattgttgct gctattaaaa ataatccaat gttttcttta ttaagtgaaa acaaagaata
    11281 caaatttctt atagaaaaat tatctttatt ggaggaataa gattatggaa aatttgatgg
    11341 aatatttacc acttttgatt cctgttatta ttttagattt aatacttata attactgcac
    11401 ttgttcatgt attgagacat ccaaattata aaattggaaa taaggcaata tggattattg
    11461 tagtactgtt tattagtcta ataggaccaa tcttgtattt tacaatagga agaggtgaag
    11521 aatgatgaat attgtctcaa taaatggatt atcaaaaggt tttggtaatc gaaagataat
    11581 agataatcta aattttactg taccagaagg gtcagtcttt gggtttgtag gtaagaatgg
    11641 tgcaggtaag actacaacaa tgaaaatggt acttgggtta cttgaaccag atagtggaac
    11701 tatagatgtt tgtggtgaga aagtgacata tggaagaaca agttctaatc gtcatgtagg
    11761 ttatctgcct gacgttccag agttttacaa ctatatgaga ccacttgaat atctttctct
    11821 ttgtggtgaa attacggggc tttcaaaaaa agaaataaag ataaggagtg aagagttgtt
    11881 atcacttgtg gggcttggca atgaaaagag acgaataggt gggttttctc gtggaatgaa
    11941 gcaacgactt ggtatagctc aagctttgct ttcacgtccc aaattactta tttgtgatga
    12001 accaacaagt gcacttgacc ctgttggtcg aaaagaaata ttagatatta tgcttaaaat
    12061 caaagactca acaactgtaa tattttcaac acatatacta tcagatgttg aaagaatctg
    12121 tgaccatgtt gctatactaa acaaaggaag cattgctctt agcggtacac tttcagaaat
    12181 aaaaagtatg cacggaaagg atagactttt actggagttt gcaagtaata atgaaattca
    12241 aaagtttaag tctagtggtg gcattaaatc cttactaaaa gattcagaag aaactaatat
    12301 ggaaattgtt ttgcatggta aagatataaa agcaatacaa aaaacagtta tatctacact
    12361 cgcagaaatg aatctttgtc cagtaaagat ggaactaata gaattgtcac ttgaaaattt
    12421 gtttttggag gtagtaaagt gagagaatac atagcattta caaaaaaaga gtttaaggag
    12481 aacataagaa attataaatt acttagtttg actattttat ttttagtatt tggaataatg
    12541 agtcctcttt ctgcaaaatt tatgccagac ttgattgctc attttgctcc aactttaaaa
    12601 gttactactg caccaactgc tttagattct tggacacagt tttctggaaa tatttctggt
    12661 cttggaacga gtttaactct aattgttttt tgcaatattt tatcaaacga atattctaaa
    12721 ggaacactcg ttattatgtt gactaaaggg ttatcacgtt catctgtagt attgtctaaa
    12781 ttttctattg cagtaattat tatgacaatt ggtttttggt tgagcttttt atgtacttat
    12841 ggatatacaa tgtatttttg gccaactgca aatcttaatc atattatatt ttcagcattt
    12901 aacttatggt taattagtat tatgtatatt tccatattaa ttcttgggtg tgtactattt
    12961 agaccagcat ttgcaagtgt gttacttgta ttagcagcaa ctgctatttt gagtttgatt
    13021 tctattccta aacagattgc accttatacg cctaatttca tattatcaaa aaatatagat
    13081 ttagtttcag gtaaagtgac acctccagaa tttattattc caatcattgt aacaattgtt
    13141 atttcgatag tatgtctgtt attagctgtt gttttgttta ataaaaaagc agtttaagga
    13201 tatttttatt ataaatacaa atagtgctgt attgtgggaa aatgtgttta gtattgtaga
    13261 agaatatatt taagtaaaaa agaggctgtc tcaagataga gaaataagcc tcttttttat
    13321 tttagtcctc ctcactatac tttataataa ttaagttatt accttaagga actatctaa
//
LOCUS       DAEPLF010000085        13337 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEPLF010000085 DAEPLF010000000
VERSION     DAEPLF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.085"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            168..1745
                     /locus_tag="KR314_002110"
     CDS             168..1745
                     /locus_tag="KR314_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBH0328493.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVENLEAKVDSIEKAIDSISQKVN
                     KIQDILDKIGFLKKFLS"
     gene            1879..3228
                     /locus_tag="KR314_002111"
     CDS             1879..3228
                     /locus_tag="KR314_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBH0328494.1"
                     /translation="MGIGIECNKKINKSIDKTRIKKNNLNSNSNSNSNSNSNSNSNSN
                     SNSNSNSNSNSNSNSNSNSNSNSNKISNNVVDSKLTNSRIKSIDIIRGLSIALMIVCN
                     NPGTWMRMYPQLRHAVWHGVTLADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILS
                     IFKRSILLILFGFFLNYLGNPDLNSVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTA
                     TIITFLCIATFIIVGYYILAKPYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSI
                     VAISSGMLGCTMGCVLLKENIGEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSS
                     FVLLMAGSYGMLLSIFYFICDIKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPK
                     LTNKVDYPTTLVEWTTYELITPWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            3482..5506
                     /locus_tag="KR314_002112"
     CDS             3482..5506
                     /locus_tag="KR314_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0328495.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAGT
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            5726..6412
                     /locus_tag="KR314_002113"
     CDS             5726..6412
                     /locus_tag="KR314_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBH0328496.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            6507..7562
                     /locus_tag="KR314_002114"
     CDS             6507..7562
                     /locus_tag="KR314_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBH0328497.1"
                     /translation="MIKKISTILSLVLLISISSTIEVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGIKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTAKLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            7677..9236
                     /gene="murJ"
                     /locus_tag="KR314_002115"
     CDS             7677..9236
                     /gene="murJ"
                     /locus_tag="KR314_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBH0328498.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDDKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRRR"
     gene            9278..10984
                     /locus_tag="KR314_002116"
     CDS             9278..10984
                     /locus_tag="KR314_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBH0328499.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNDTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            11006..12079
                     /locus_tag="KR314_002117"
     CDS             11006..12079
                     /locus_tag="KR314_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBH0328500.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEYISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            12091..12813
                     /locus_tag="KR314_002118"
     CDS             12091..12813
                     /locus_tag="KR314_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0328501.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            12829..>13337
                     /locus_tag="KR314_002119"
     CDS             12829..>13337
                     /locus_tag="KR314_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBH0328502.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNIDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTI"
BASE COUNT         5318 a         1442 c         2447 g         4130 t
ORIGIN      
        1 gttttatatt tttatcattt aaataatgaa aaataggctt gtctatattt ttagatagtt
       61 ttcttattat tgatgaaaaa tgtaataatt ttaaactgta aaatagtgta aagttatttt
      121 ataatataaa tatagtaaga ttttaaacaa aataaggggg aataataatg aaagcaccaa
      181 aaactatttt aacaatactg acaatagcgc ttactttaag tagcatttct ataataccat
      241 catatgcact tacagaggaa aaattaatag gtaatggtag atatgaaact gctgtaaaga
      301 taagtcaaaa ggcatacagt tctagttcta atgtagtgtt agttaatgac aattcattag
      361 ctgatgcact ttcagctaca ccatttgcaa aggctaaagg agcaccaata cttttgacag
      421 aaagtgataa gctagatgat aggactgaaa aagagataaa acgtctagga gctaaagata
      481 tttatttgat aggaggtact gctgtactta ataaagatat agagaacaag ttaaaaggta
      541 atggacttaa tgtagaaaga attaatggta aggatagata tgaaacttca ctgattttgg
      601 ctaataagct taaggatata aaagatatta aggaagttgc tgtagtaaat ggtgaaaaag
      661 gattaagtga tgctgttagt gttggagcac cagcagctca aaataagatg cctataattt
      721 tgtctagtcc taaggacgga gtagaagcat ttgataaatt tataagagat gagaaagtta
      781 taaaagcata cgtaattgga ggaacaaatt ctgtttcaag agctgtagaa aagagccttc
      841 ctaatgcaga aagagtgagt ggaaaagata gaaatgagac taatgccaaa gttatagaaa
      901 agttttatac tgatacaaat ttaagtaatt tatatgtaac taaggatggt agtaaaaatg
      961 agaatcaact aatagactcg ttagctgttg gagtattggc tgcaaaaaat gagtctcctg
     1021 ttgttttggt tggtaataaa ttaaacacga aacaaaggga tatacttagt actaaaaaat
     1081 taaatactat aacacaggta ggtggaaatg gaaatgaaga agcttttgat gaaattaaga
     1141 gtttacaaga aaaaactgtg tttgaagcaa aaactgttga ggaattaact gatatgataa
     1201 atatagcaag tcctaatgat attataaact ttaaaccaaa agagaatact gtaaatgaag
     1261 cttttagaat ggtaacaaat aaaccaataa ctgtgaatat aaagggtgat tgttcaaaga
     1321 ctcttacagt agatatgcct aatggtgaag tgaataacta tgctactttg gtaaatgtta
     1381 tagttagaaa tattggagaa ggtggattta ataaccatga tacaatcact atattgtcag
     1441 ttcgtgacaa aaatggtaga gttatagaaa atactagaaa ttcagatata gatacgttga
     1501 tgatattagc ttctgctaat gatacaaaat tgattaatga tggatatata ggaaagctta
     1561 tagataattc atctaatagt gatataacta ataatggtac tatagataaa aaagttaatc
     1621 aagttgagaa tttagaggct aaagtagatt caattgaaaa agctatagat tctataagtc
     1681 aaaaagtcaa taaaatacaa gatatactag ataagattgg atttctaaag aaatttctta
     1741 gttaagaata tgtatattta agtgaagtgt gtgttgtaat tgttctaaag ttatatagtt
     1801 aaacaggttt atatacaaac agacttatag ataaatatgt agtttagtat atatttgaat
     1861 aagctttggg gaggtaacat ggggattgga attgaatgta acaaaaaaat aaataaaagt
     1921 attgacaaaa ctagaataaa gaaaaataat ctaaatagca atagcaatag caatagcaat
     1981 agcaatagca atagcaatag caatagcaat agcaatagca atagcaatag caatagcaat
     2041 agcaatagca atagcaatag caatagcaat agcaatagca ataaaatttc aaataatgtt
     2101 gtagattcta aacttacgaa tagtaggatt aaatcaatag atataataag agggctatca
     2161 atagccctca tgatagtctg caataatcct gggacttgga tgagaatgta tcctcaatta
     2221 agacatgctg tatggcatgg tgtaacactt gcggattttg catttccttt ttttgttata
     2281 tctttaggtg ttacaattcc aatatcaata aatagtaaac taaaaaataa taaatccaca
     2341 ctgagtataa tacttagtat attcaagaga agtatcttat tgatattatt tggatttttc
     2401 ttaaattacc taggaaatcc tgatttaaat tctgtgagaa tacttggagt acttcaacgt
     2461 atgggattag tctattttgt gactagttta gtatatttac tactaaaaaa attaaatgtt
     2521 ggaagtactg ctacaataat tacttttcta tgtatagcta catttataat agttgggtac
     2581 tacatattag caaaacctta tggatttgag ctagaaggtt ctcttgcaca gttagtggat
     2641 ttacatttct tcaagggaca tttgtacaag ccagaatttg aaccagatgg ttttttaact
     2701 agtatagtag caatttcttc aggtatgttg ggatgcacta tgggatgtgt acttttaaaa
     2761 gaaaacatag gagaatataa aaaattcttt aaaatactgg ttatgagcat aatcctgcta
     2821 ataggtgctt ttatctttaa tcagtatttt ccatttaata aaagattatg gtctagctca
     2881 tttgtacttt taatggctgg cagttatggt atgttactgt caatatttta ttttatatgt
     2941 gacatcaaaa ataaaagtaa aatatttacc ccaataatag cacttggaag tagccctatt
     3001 tttacatata tgtgccttga gatattgagt catgtttttt ggaatgttcc aaaacttacg
     3061 aacaaagtcg attatccaac taccttggta gagtggacta catatgaact tataacacct
     3121 tgggcaggta caacttggga ttctcttata ttttctttat tgtatgtcct attctgggtt
     3181 atagtaatgt ctataatgta caagaaaaaa atatttatta aaatttagtg caatattttt
     3241 agatgataaa gtctttgatt tgttcaaata tacggaaact atcaaatata atcaaaattt
     3301 ctcctcgata aaatttaaaa accatgccat aaaagattac aaaaagttta caatttgtaa
     3361 ttttttgtgc tataatgaaa aaagatttac ataatattga atttatgtaa tataaaacaa
     3421 gcttaagatg tattaaaaaa gatttaaaga tttaaagaac caaaaaatta ggggggatat
     3481 gatgaaaaaa acaacaaaat tattggcaac aggaatgttg tctgtagcaa tggtggcacc
     3541 aaacgtagct ttagcggctg aaaatactac tgcaaataca gaatctaatt cagatattaa
     3601 tataaattta caaagaaaaa gtgtagtgtt aggaagtaaa tctaatgcaa gtgtaaaatt
     3661 taaagaaaaa ttaaatgctg attcaataac attaaatttt atgtgttatg atatgccact
     3721 tgaagctact ttaaattaca atgaaaagac agattcatat gaaggtgtaa taaactataa
     3781 caaagaccca gaatacttaa atgtatggga acttcaaagc ataaaaataa atggtaaaga
     3841 tgaacagaaa gttttaaaca aagaagactt agagtctatg ggattaaatc ttaaggatta
     3901 tgatgttaca caagagttta taataagtga tgcaaattca acaaaagcag taaatgaata
     3961 tatgagaaaa acttctgcgc cagtaaaaaa acttgctgga gctactagat ttgaaactgc
     4021 tgttgagata agtaaacaag gttggaaaga tgggtctagt aaggttgtaa tagttaatgg
     4081 agaattagca gcagatggaa ttacagctac tccacttgca tctacatatg atgcaccaat
     4141 attacttgca aataaagatg atataccaga aagtacaaaa gcagaattaa aacgtctaaa
     4201 tccaagtgat gtaataatta taggtgatga tggttcagtt tcacaaaagg cagtatctca
     4261 aattaaatct gctgtaaatg taaatgtaac tcgtattggt ggagttgata gacatgaaac
     4321 atctttactt atagcaaaag aaatagataa gtatcatgat gttaataaaa tatacatagc
     4381 aaatggatat gctggtgaat atgatgctct taatatttca tcaaaagctg gtgaagacca
     4441 acaacctata atattagcta ataaagactc tgtaccacaa ggtacatata attggttatc
     4501 ttcacaaggt ttagaagaag cttattacat aggtggtagt caaagtttat catcaaaaat
     4561 tatagaccaa atatctaaaa tagcaaaaaa tggtacttca aaaaatagag tttctggagc
     4621 agatagacat gaaacaaatg caaatgtaat taagactttc tatccagata aagagttgag
     4681 tgcaatgtta gttgcaaaat ctgacataat agttgactca ataacagcag gacctttagc
     4741 agctaaatta aaagctccaa tacttataac tccaaagact tatgtttctg cataccatag
     4801 cacaaactta tcagaaaaaa cagcaggaac agtatatcaa attggtgatg ggatgaaaga
     4861 tagtgttata aattctatag catctagttt atcaaaacac aatgcaccaa cagaaccaga
     4921 taattcgggt tcagcagcag gaaaaactgt agtgatagac ccaggtcatg ggggttctga
     4981 ttcaggggca acaagtggat taaatggtgg agcacaagaa aagaaatata ctcttaatac
     5041 agcattagca actactgaat atttacgttc aaaaggtata aatgttgtta tgactagaga
     5101 tacagacaaa acaatggctt taggagaaag aacagcatta tcaaacacta taaagccaga
     5161 tttatttaca agtatacatt acaatgcgtc taatggagct ggtaatggtg tggaaattta
     5221 ttacaaagta aaagataaaa atggtggaac aactaagact gcagcatcaa atatcttaaa
     5281 aagaatacta gaaaaattta acatgaaaaa tagaggaatc aagacaagaa cacttgataa
     5341 tggaaaagat tacctatatg tgttaagaaa taacaattat ccagcaatac ttgttgaatg
     5401 tgcatttatt gacaataaga gcgatatgga taagttaaat actgcagaaa aagtaaaaac
     5461 aatgggaact caaattggaa taggaataga agatacagta aaataaaata aaaaatatta
     5521 aagcaaaaca tatcatctcc tgttataatg taaaatataa caagagatga tatatttttg
     5581 taaagaatca aattgtttac aatgatgttt cttgtattga taatctgctc attattgagt
     5641 ataatataga aataagtcaa aaataagata gaaataagtt gaaattaaga attaaaaaaa
     5701 tttttattcc aatatggagg taactatgtc tggatatact aatgatgagt gtgaaatacc
     5761 taaaattaaa agctatccag gggcagataa agaaattgca agtgagattg actatagcat
     5821 agtaaaagga acggttttat tcgacttgta ccaaagaatt atggatttag ttttatctat
     5881 aataggactt gtgataggtt tacctttaat agcaatattt ggaattctta ttaaaataga
     5941 ggacaaagga ccaataactt ataagcaaga aagactagga aaatgcggga gaagattcta
     6001 tatatataaa ttaagatcta tgagaacaga cgcagaaaaa tttggcgcac aatgggctga
     6061 aaaagatgat cctagaataa ctaaggttgg aaagtttatt aggaaaacta gaattgatga
     6121 gattccacag ctgtttaata tattaaaggg ggatatgggc ttaataggcc ctagaccaga
     6181 gagaccgaac tttactgttc aattcaatga ggaaatacct ggatttatca acaggctcgc
     6241 tataaaacct gggcttacag gttgggcaca agtaaatggt ggatacgaaa tcactcctga
     6301 agaaaaactt aaagaagata tttactatat aaaaaatagg agtatattac ttgactttaa
     6361 gatactcttt aaaactgtta aagtagtatt gacaggagac ggagctagat aattttaaaa
     6421 tattagatta ctaatcaatg aaaaagctct ttaaaaagag cttttttatt gtcaaaaaat
     6481 acaaaaaaga aaaggaatga gaacttatga ttaaaaaaat ttctactata ttatcgttag
     6541 ttttgttaat ctctatttct agcacaatag aggtatttgc agatgcaaat cccaaaagag
     6601 aattgataga agggagtata ccagagattt cgacagaatt aaataaaaga gcatttaagg
     6661 attctaaaga agtaattctt gtaaatgaag agtcaattgt agatagtata agtgcaacac
     6721 ctttagcata tgcaaaaaat gctccaatag ttgtaactaa gagtaaaaat cttggtagag
     6781 taacaagaaa ttatctaaaa gaactaggtc cagagaaggt tacgatagta ggtggtttaa
     6841 aggcagtatc taaagatgct gaacgtaata tagaaaaaat gggaataaaa gtagaaagaa
     6901 taagaggaaa agacagatat gatacatctt taaagatagc tagagaaatg tatagaacag
     6961 taggatttga tgaagcattt ttacttagct caacaacagg attagagaat gccatatctg
     7021 tatattctta tgccgctaaa agtggaatgc caataatatg ggctaaagat gaagggtttg
     7081 aggaacaaat tgatttctta aaaggtaaga atcttaaaaa aatatatgca ttaggtgatt
     7141 caaaagaatt tattgcagag attgattcta acttgaaaaa tatagaagga ataaagcaaa
     7201 taaataagtc aagtacaaat gttgatttaa taaagaagtt ttatgatgaa aaagatataa
     7261 agaaaatcta tactgctaaa ttagactttg gtagcagatc tgatgtaaac gaatatattt
     7321 ctttgggagt agtatcagca aaagaaaata tgccaatact gatttgtagt gataacttaa
     7381 gtcgtgcaca agataaattc ttgaaagata gcaatataaa tgatgtagtt gaagttgggt
     7441 atacagtagg tgattatagt ctatttaaga gcatatttaa tctaacattc ttatcttgta
     7501 tagtattaat actattatta ctattgatta catttagggc acttagatat gagtcgaaat
     7561 agtttttaat ttataaggaa gcttttcata ggttaaattt acttaattag aataatgttt
     7621 ataagcagtt ttaaaagttc tacttttaga gtttaaatta gataaggaga aagaagatgt
     7681 caaaaacagc taaagcagca ttgtggatta tggcagccac tatgttttca aaagtattag
     7741 gtttccttag agaacttgta cttgcaaatt tctatgggac aggtatgtat gcagacgtat
     7801 ttgtattaac attaaatata cctggtttaa tcatagcagt cataggctct gcagttgcta
     7861 caacttatat acctatgtac tttgagacaa agaaaagact cggagatgaa ggtgccttaa
     7921 aatttactaa taatgtttta aatatatgct atataatggc tatagtaata gctattattg
     7981 ggcttttatt tacagagcaa tttgttacag tatttgcagc aggatttaga aacgaccctg
     8041 ctaagttcca agcagcaata ttatttacta aaataatgat ttcaggagtt ttattcctaa
     8101 gtggaagtaa gatatttagt tcgtttttac aggtgaatga cagttttgta attcctgggc
     8161 ttataggaat cccatataat attataataa tagcagcaat agcactaagt gcaggtaaaa
     8221 acgtatggat actaccagca ggagcactat tggctatggc gagtcaacta ttatttcaac
     8281 tgccatttgc atttaagaag tcctacaagt ataagccata tataaactta aaggatgagt
     8341 ctataaaaga attagtaaac ctagtattgc ctatgttggt aggagtagct gttggacagt
     8401 taaatatatt tgttgacaga ttattggcaa ctactttggg tgatggaaag ttgtctgctc
     8461 ttaactatgc aaataggcta aatgagtttg ttatggcatt atttgttaca tctataataa
     8521 cagttatata tcctaagcta gcaaaaatgt ctggtaaaga tgataaagaa ggatttataa
     8581 gtactattgt aaaatcttca aattgtatta tattggtggt gcttcctata tctataggag
     8641 ctattatatt agcagagcct cttgttagga tattatttca aaggggtaaa tttgatgcgt
     8701 tatcaacaga tcttacatct atagcgctta gattatattc actaggttta ttggcttgtg
     8761 gagtaagaga tgtcttatat agggcatttt actctctttc agatacaaaa acgccaatga
     8821 taaatggaag tatagcttta ataataaata tagttcttaa tttaatattg ataagaccat
     8881 taggtcatgc aggtattgct atatcaacta gtacatccaa tataattaca gttatattgt
     8941 tatttatatc tcttaaaaag aaaaatggat attttggagg ggataaaatt ataaagactg
     9001 gtctaaaaag tttggtagca tctggtgtga tggcagtagc tactttattg atatataata
     9061 atctatatgc atttatgggt tcaggaacaa ttaaagaaat tatatcagtt ggagctggag
     9121 tattaggtgg agcttctgta tatactgtat tgatagtttt atttaaggta gaagagatgg
     9181 acttagcttt tgaattttta agaaaaggaa aacaaaaact acttagaaga agataattac
     9241 cttgaaattt taaattagag tcagaaagga tgaaaatatg gattataaga ataattatga
     9301 gatgtggtta aattctcctt attttgatga gcaaacaaaa aatgagctat taagcataaa
     9361 agatgatgaa aaagaaatac aagatagatt ctataagaat ttagagtttg gaacaggtgg
     9421 tttaagaggt ataataggag cgggaactaa tagaatcaat atttatactg ttagacgtgc
     9481 gacactgggc gttttaaatt atatcatgaa gactcaagga gaagaaggta aacaaaaggg
     9541 aatagttata gcacatgata gtagatacat gtctagggag ttttgtatag aagtagcaaa
     9601 gactttaagt gcttatggtg taaaggctta tatttttgaa gaattaaagc ctacaccaga
     9661 actttctttt gctgttagat atttaaagtg tgctatggga atagtaataa cagcaagtca
     9721 taatcctaaa gaatacaatg gatataaagt atatgactca gatggtggtc aaatctgtat
     9781 agatatggca aatgatataa tagcagaagt taataagatt gatgattaca gcactattaa
     9841 aagtatagat tttgaagaag ctttatctaa aaatttaata actatattag ataatgaagt
     9901 agatgatgag tttataaaag ctgttaagaa gcaagtttta agacaaaata taatagatga
     9961 atacgggaaa aaattaaaaa taatatatac acctattcat ggtacaggaa ataaaccagt
    10021 aagaaaagtc ttaaatgaat gtggttttga aaatgtaatg gtagttaagg agcaagaatt
    10081 accagattct aatttttcaa cagtaaagta tcctaatcct gaagaaaagt cagtgtttaa
    10141 tatagctatt gaaatggcaa agaataatga cactgatttg ataataggaa cagaccctga
    10201 ttgtgataga gtaggtatcg ttgtaaaaga ttcaagtggt gaatatgtag tcttaaatgg
    10261 aaatcaagtc ggttcacttt tagttagata tatattagag agtttagttg aagaaaataa
    10321 acttcctaaa aacaatccta caataataaa aactatagtt acatcagaac taggtgcaaa
    10381 aatagcaaag gcttataatg tagattgttt aaatacttta acaggattta aatttatagg
    10441 agaaaaaata aaagcgtttg aagaaagcaa tgatagaagt tttataatgg gatatgagga
    10501 aagctatggt tacttaattg gaactcatgc tagagataaa gatggtgttg tatcttcact
    10561 tatgatatgt gaaatggcag catactattc ttcaaaaggt atgaacttgt atgaagcttt
    10621 gatagataca tataataaat ttggatacta caaagaggac ttaaagtcag taactttaaa
    10681 aggtatagat ggaataaaaa aaataaaaga gatgatgctt tactttagaa gtgttaaaat
    10741 agataatgtg gctgatgtaa aagtagataa aattttagac tataaagatg gtgttgatga
    10801 tttacctaag tcagatgtac ttaaattttt acttgaagat ggctcttgga tagctataag
    10861 accttcaggt acagaaccta aaattaaatt ttactttgga gcaaattcag acaatcaaga
    10921 agatgtagaa tttaagttga acaatttaat atcttatata ttaaatgtag tggattctat
    10981 ataaaaagct aaggggagat aaataatgaa agtatacaat gtcataatgg ctggaggagg
    11041 aggaactcgt ttctggccac ttagtagaca agaagtacct aaacagctta taaatttaag
    11101 tggagaagat gctttaataa acgaaactat aaacagaata gattctctag caaaaaaaga
    11161 tgatttattt atagtaacca atgagaagca attggaagct ttaaaggata tagtaaaaga
    11221 taaatgtttg gatagtaata tattgccaga gccatgtgcc agaaatacag cagcagctat
    11281 aggctttgca gcatttaaca taatgaaaaa atatggtgat ggtgttatgt gtgtatatcc
    11341 agctgaccat tatataaaag atgaaaaaga atttaaatct attttggaaa aagctatcta
    11401 tatagcagaa aataatgata aactagtaac tattgggata actcctacat ttccatcaac
    11461 aggatatgga tacataaatt ttaatagaga aaatacgata gaagatgttg catatgaggt
    11521 agtggagttt gtagaaaaac caaactatga aattgctaaa gaatatgtaa actctaaaaa
    11581 gtatgtatgg aatagtggaa tgttcgtatg gaaggtatct aaaatattag aagattttaa
    11641 gagatacttg ccaaaagtat atgaaaaatt agaatatata agtaaatact taggaacaaa
    11701 agaagaaatg gaaaaaataa aagaaatata cccaactata caatcaatct ctatagatta
    11761 tggtattatg gaaagaagta atgatgttat agtagttcct ggtgattttg gatggaatga
    11821 tgttggttct tgggattctc taggtgcaat atatccaact gatgatgaag gtaacatcaa
    11881 aagaggtgaa aatattacta tagatactaa aaattcaatt atatattcag atgacaaatt
    11941 aatatcaaca ataggtatta gtgatttgat agtagtatct acgaatgatg cagttatggt
    12001 ttgtagaaaa gataaagctc aagatgtcaa aaaaatagta gaacagttaa aagaagaaga
    12061 tagacaagaa tatatgtagg aggtgttcat ttgattccta aaaaaataca ttatgtatgg
    12121 tttggtggtc caaaaggaaa tatagaaaat atatgtataa attcttggaa agaaaagctt
    12181 ccagaatatg agatagtaga gtggaatgaa aaaaactttg atatagagaa agaaataaaa
    12241 ggaaataagt ttttggaaga atgttataaa agaaaacttt gggcattcat atctgattat
    12301 acaagaataa aagtactcta tgaacaaggt ggagtttata tggatacaga tatgcaaata
    12361 ttaaaggata taactcctct tttagaaaat aataggctaa tctgtgggta tgaagatgat
    12421 agagagtata taaatggtgc tataataggt gtagaaaaag gacatccttt tttaaaagac
    12481 ttattggagt attatgaaaa agaagtactt acttcttcat tgtttacaat accaaaaata
    12541 atgactcatc ttatggaaaa gaattataag aagattgacc caaataatta tgaagaagga
    12601 atacgtgttt acgacaaaga atatttttat ccttttggat ttaaagaaga ctttacacca
    12661 gaatgtataa cagaaaacac ttttgggata cattggtggg gaaaaagttg ggctaaaaag
    12721 agaaattatt tcttagaaag caaacactta acaggtgtaa ataagatatg gaagtgttgt
    12781 aaaatatttg caagtaatac tttacgaagc taaaagggag aaagtaaaat gaagacttta
    12841 aatgatgata gaaatattat gattagaaaa tatctttata tatttgtttt gtttatcata
    12901 ttacttagaa agtatatgat atcatttatt gacccaaata tagatatagg gatgattaaa
    12961 tcagcgttat tttattcttc aataggaata ctaatgctac tttttttatt tgataagaga
    13021 aaaagtataa cagaaatggt actagttgga gtttgtgtct tactttattt attaaatagg
    13081 gaaggagcta tattattaat cgttttatta gctgtatcag ctaagcaaat agatgataaa
    13141 tttatagtta aaaattattt aataatatcg gcatgttttt taatggtagc tatattatta
    13201 tttaatttat ttccttcatt aatatttaat caggaagtcc actatagata tatagaaaaa
    13261 atagatatgc ttgttacaag aatggatttt ggacttggta atccaaatag tgtattttat
    13321 catatggtta ctatata
//
LOCUS       DAEPLF010000086        13128 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEPLF010000086 DAEPLF010000000
VERSION     DAEPLF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13128
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.086"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            151..591
                     /locus_tag="KR314_002120"
     CDS             151..591
                     /locus_tag="KR314_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBH0328503.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLETQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVSLTPKA
                     KELWVTRYTCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(776..964)
                     /locus_tag="KR314_002121"
     CDS             complement(776..964)
                     /locus_tag="KR314_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328504.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
     gene            2025..2420
                     /locus_tag="KR314_002122"
     CDS             2025..2420
                     /locus_tag="KR314_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328505.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNNIKCEEIKDNVIEEKNNTCTTE"
     gene            2463..4562
                     /locus_tag="KR314_002123"
     CDS             2463..4562
                     /locus_tag="KR314_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBH0328506.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEISVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLKKDATEILVPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTGIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNHSIAVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKKDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLREYGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVSGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYY"
     gene            complement(4787..9997)
                     /locus_tag="KR314_002124"
     CDS             complement(4787..9997)
                     /locus_tag="KR314_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBH0328507.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRNFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDRQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKTQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLCIPEGIYSINVSGK
                     DAEKLVGEGSIYDGMNGRNGNNAGTSGDILLSFKEIEKRGELKLYVDGGRGGNGQDGK
                     RGDTGITGEAGENAKIGSKKESKEFAKVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKASLLKGAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSAPIDDSDRNYYYFDRYVFNHASNENQVADDGEKLNALYEKHKDWTISKREDSGLV
                     GAEGKDGRSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFIGEKKNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKDELENK
                     MLSVNAKMNLLIQEIIKMKEEEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYAEKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSGSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYIDI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSESIDILKSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIC"
     gene            complement(10012..12135)
                     /locus_tag="KR314_002125"
     CDS             complement(10012..12135)
                     /locus_tag="KR314_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0328508.1"
                     /translation="MQNSVKQEPCDFSHGRFRPEADILDSRLTKVLDLFQQLNMCLHK
                     DNFRGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFEELLKDNGK
                     NIHLCKPLESDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIKEIQNNIVS
                     SLEDYVKAYTEDFISIIGSITMIQEIAKYDEVVKVFKNWLESTTTLTIERFINVLAKQ
                     SGLFLQDSCMELQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEKASKKIMEQ
                     NFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSILTNVTVTQ
                     NEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIRALIPNLVI
                     SSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILACNNLDVGSNVKI
                     NVSGKSGELVDSSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNGSKGIDGKN
                     GETGDSGSNGADAGYTSSFHGQMFKGYFAKGTSGKKGDKGGAGGNAGRGGNAGNKGNI
                     YFISDSEEQKNRIINHCDITVKDGEAGPNGRPGSGGYGGSGGYHGYNTLSFSPSGIDK
                     TVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDDGGFNAPNT
                     KLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(12200..>13128)
                     /locus_tag="KR314_002126"
     CDS             complement(12200..>13128)
                     /locus_tag="KR314_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0328509.1"
                     /translation="KELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKFKSKKTN
                     RFSYKTNFTNGNIMYFSQHIKLPKLGMVKIRDKQVPQGRILNATISKEPSGRYYVSLC
                     CTDVDIEAFENTNNQIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQRELSRKT
                     IGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIKNRKL
                     SRLISDVSWSEFIRQLKYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLSIREW
                     ICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         4311 a         2351 c         1507 g         4959 t
ORIGIN      
        1 ttccaatata tataaatatc atatttttct attagctaaa aataattact ttatattttt
       61 acatgttagt ataataagac ataaaaactt gacatactgt tgtcataatt actttgctat
      121 aattaaaagt aaaaatggag gtaaattata atgtatgaac gtatgcttga taaaagcaat
      181 aaaccctgtt tgtctcaaat aatagaatat ataggtaaag atggatatgc acgattgtgt
      241 aagttggaaa cttacttgga aactcaatat aatttatcaa gagaacttcg atttcctttt
      301 ggaaacaatt atggttgggg ttacaaatat agccataaat cttttcattt attttatgct
      361 ttttttgaaa aaggtgcatt tactgttctg attcagatag gtgacaagca agttccagca
      421 gtagaaaaaa cgttggtatc tttgacacca aaagctaaag aactctgggt cactcgttat
      481 acttgtggca ataatggagg ttggattaag tatcgtattt tagaagatgc agacttactt
      541 gatattattc aatatataag agcaaaaaaa tctccaccta aacaaaatta acttttattt
      601 tttgtgattt aaatatagtg tatataaatt ccattctttc catattaaaa aaataatata
      661 aaaaatatct attagtatat cttatttagt atgtcttatt ctacttttta cagataatta
      721 aaaagtttta tcataaataa atcataacta aacatcctag taaatcaaaa ttcatttatt
      781 cttttttatt agaatttttg ataacagtcc ctatacatat gccaattatc attccaaaaa
      841 ttagaccata acacattgtg atacccacat ttcctttaga aatacctata gcacaaccta
      901 tagccattcc aagtatcatg cctataaaca tacatataat aactaaattg ttgtttttat
      961 tcatagtttt tctccattca atatatttta aattgcctag ctcgccttct tagcttaaaa
     1021 caaagaaata taaattaact ttctttcatt ctaatatctg cttgtataaa tgtcaaacaa
     1081 cacatttctt gtcataacct cctctttttg aagtaagtac aatctcccag acacaataat
     1141 atcaatttcc taaaaccatt taagtaactt ttatatttac ataatattta caatcataaa
     1201 ctatattgtt attcatttat acatcataaa cactccctcc taatcattat tatacttaaa
     1261 ataatgttca tttataataa tatatctaac gcactttaat tgaaaaaact aaaactttgc
     1321 ctttcattcc catatataaa taagtatact ttttgatgct ctattacaaa tcaactaaat
     1381 taattgatat aaattatcat tttctgttga aaataccatt tacacatgct atacttaatt
     1441 gcataaatgg taataaatgt catatgattt tacaaatcta ttaataacaa ttattacttt
     1501 tactgtattt taaagttttt ggactaaata tcaagaactt tctaaaatac aaagtacaaa
     1561 aaatttatat aagttataaa tttatatgaa ctagataagc tcaaacaata tgttatgtat
     1621 aaactgtgta gtattcggtt ataaatttat ataaattata taagctatat aaagtattac
     1681 ctcaactata taagctagat atataaatta catacataag ctagatatat aaattgcata
     1741 tataagctat ataagaatta tttcaactat ataaactaca tatataaatt acatacataa
     1801 gctagatata taaactagat atataaactg catatataag ctatataaaa attatttcaa
     1861 ctatataaac tacacatgta aaattcatgt aaatttacta atataaatca aataaaatta
     1921 ctaaactctc taaactaatt ttgtaaaaca tactctattt ttaacatttg ttaccttata
     1981 cataagtata aacaaaatct aactaaatga aaggataatg taaaatgaat attttaaaaa
     2041 tgaaaaaaac aggaatattt ttaggaggag tactttttgg tactgtaggt gttaagatac
     2101 tttcaagtga ctgtgtaaaa aaaacttgca ctcgtgcaac agcaggagca ttgaaagtta
     2161 aagattctgt tcttgaaaca actacgaaaa ttcaagaaaa tgtagaagat attttagctg
     2221 aagcaaaaga aatcaacgag caagaagact tatctacatc taaggtaaac gttgtacaag
     2281 atactcttaa atgcgaacaa gaagttctta aaactgaaac tataaaaact gtagaagata
     2341 ctaattctaa taacattaaa tgtgaagaaa tcaaagataa cgtaattgaa gaaaaaaata
     2401 atacttgtac aacagaataa atttaatgat tattatgatt aataatatca ggaggaagta
     2461 aattgaattt ttatataaaa cacgaaataa gaggtcgtat acgctttgac ttacaacttg
     2521 gcaaattaaa caacaagcag tccgatactc tcttatatta tttaacttct ataaatggta
     2581 ttaccaaagc aaaagtttac gatagaactg gtgatgcagt catttattac actggtgaaa
     2641 gagaatacat aataagagaa atcactaaat tcagttttaa caatagcaaa atagaaaacc
     2701 tagttcctga acataccagc agggaattaa acaattatta tagagacaaa ttagcaaata
     2761 aactgctagt caaaagtatc tccaaaatat tttttccaaa gtccttaaga ttttttttaa
     2821 ttggacttca ttctataaaa tattttaaaa aaggtctctc ttccctacta aataaaaaaa
     2881 tagaaatttc tgttttagat gccactgcta taggtatttc tatttttaga aaagatatca
     2941 atacggctgg ttcagttatg tttcttcttg gtatcggtga gttattagaa gaatggacta
     3001 gaaaaaaatc tatagacgac ttggctcaaa gtatgtcact aaacattgaa aaagtatggt
     3061 taaaaaaaga tgccactgaa atactagttc caatttcaga gataaaggaa ggagatttag
     3121 tcagtgtaac tatgggaaat atgattccat tggatggagt tatagtatca ggagaagcaa
     3181 tggtcaatca ggcatcactt acaggtgaat cccttgcagt taacaaaaaa gaaggctctt
     3241 acatatatgc tggaactgga atagagcaag gcaatattgt tatgtgcgtc aaggaaaaag
     3301 ctggtactac tagattccag aaaatagtaa ctatgattga agaatctgaa aaactaaaat
     3361 cttctgtaga gagtaaattt gaacatctag cagatacatt agttccatac agctttttag
     3421 gaagtatatt aacctatgca attactagaa accctataaa atcactctct atattgatgg
     3481 tagatttctc ttgtgcatta aaactctcta tccctatttc agtactatca gccatgagag
     3541 aatgtaacaa tcacagcatt gctgtaaaag gtggaaaatt ccttgaaggt gttgcatgtg
     3601 cagatactat tgtatttgat aagacaggca cattaacaaa agctcaacca actgtttcag
     3661 atataattac attccaaaat tataataaga aggatatgtt aagacttgca gcttgcttag
     3721 aagaacactt cccacactcc atagcaaatg cagtagttta tgaagctgaa aaacaagggc
     3781 ttagtcacaa agaaatgcat acagaggtag aatatattat tgctcatggc atttcaacaa
     3841 aggtcgatga taaaaaggtt gtaataggta gcaaacattt tgtatttgaa gatgagaagt
     3901 gtactatccc agacggtgag gaggataagt acaacaatct atcagatgag tattctcacc
     3961 tatttatggc aatatcgggt aaattatcag cagttatttg cataaatgac cctttacgaa
     4021 aagaagcaaa atatgtaatt tcaaatttaa gagaatatgg aataaaaaag attgtgatga
     4081 tgactggaga tagcgaaaaa acagctaaat ctatagcaag taaagttggt gttgatgaat
     4141 attattcaga agtcttacca gaagacaaag ccaattttgt taaaagtgaa aaactaaaag
     4201 gtcgcaaagt aattatgatt ggtgatggaa tcaatgattc tcctgctatt tcagaatcag
     4261 atgtcggaat tgcaatgagt gaaggtgctg aaatagcaag agagatttca gatattacca
     4321 tatcagcaga taatttgaat aatttaatcg ttttaaagca gattagtaat aagcttatga
     4381 aaagagttga tttaagttct aagtttatta tcggctttaa tttaggtctg attttacttg
     4441 gcgtaagtgg atttgttaga ccaagtacat ctgccttttt acacaacgca tctactgttg
     4501 gaataagttt aaacagtatg acaaatttat tggaaaacac caatacttat aactactatt
     4561 aatccccatt atataaaaca tatgtatata aaaaatttat aattcatctt ttaaaaagaa
     4621 actgtcttaa aaggagcttt tttctaatca aaattatata aaacatattt tacaaatttt
     4681 aaactcaact tatttctatt ttttacaaaa aggagatgat tcaaattttc attttgaaac
     4741 atcccctttt gtaaatttct ttaaaactta tatattaatt atattttcaa caaataattg
     4801 tattttcaac caatctttct tcatagcatt ttaaatcatt ttgctttaga taattaaatt
     4861 cagactctac ataaatacca cagcctacca attcaattgt caaattaggt atattttcct
     4921 ttagtatact agtctgctca taaataattc tttccaataa atcaacttgt aatatagaaa
     4981 aatgcttcat aatctcccca tcactatcta tatctttctt gatttcatac caactctttg
     5041 acaaaattcc cttcaaatca atatacccta gactcttcaa tatatctata ctctcgctcc
     5101 atgcatactt actaggttta tcaaagtatg attctagttt cttcttctgc tcatttgtaa
     5161 gtgaatcata agaaagcttc ttattaaaat gtgccttcaa agaaatctta gccatagaat
     5221 atggacttct accagagttt aatcctgcat ttttgaactt ttcaattcct ttatcttcta
     5281 ctgaagacca tattgagttt attgaatctg aatcattgta tttaacatca agaccatgtg
     5341 taatatctat atagttttgt ggaaatacat attgataata gacatctttt ttatcatcat
     5401 atagggagaa gtatccacta tcatataaat gaatatgcac ctttactttt aaactatcct
     5461 ctaaaatatt tcgtctaagg ttcgggatat ttgcatacat ggttacatat tttatggcat
     5521 caaagttttt atttaatgga acttcaaaac taatttcatc tcctgtcaaa agctttttca
     5581 cttcttcttc attacaatca tatagagaat atgtattttc aaatcccaca cgtgatttcc
     5641 catctaaact gcctgttaat gctaccttca aaatatggtt atccgttttt tgccattggt
     5701 aagcatcttg gtcaagaaac tctctaatct gtctattttg ctctttcgcc tgatttataa
     5761 cctcgttttc atcttctgta atacctacat ggcatctatt taagatattt actatattgc
     5821 ttccaaatgg aaacgtccat agttgaaaag ctgctgcctc aacctgacac aaatatctaa
     5881 cctgatttaa ttcaatttga tggataagtt ttttctgata ttcattcatc tgattaccac
     5941 tacctgacat ttgataaatc aattctccca ttgcaagatt atcttttttc ttatcaatct
     6001 tatcagtaat gataatttga gtttccatca tatcctgtac ttttttaaat attatagaaa
     6061 tctcctctga attttccatt tttaagcttc caaactttgt gcttaattca gttaaaactt
     6121 ccttcatctt taatttactt atctcatttt caaaaatatc ttgattttta actgtactat
     6181 cgaatttttc tacaaatggt ataacagatt ctttcaaagt tttatcttta aattccacca
     6241 tttgagtatt caattcatct aatttcttaa tattattttc tttatcttta attgcctctt
     6301 taactacctc tattttactc tcaaaactag attttacatt aagacaatta ttaacaccac
     6361 taatcgcagt atttataaga gaacaagcag aacttccaat actcaatgca tttttaattt
     6421 tagatgtctt ttgctgtctt tcaatagctt gtttactttt ctctagtgca gctaacttat
     6481 ctttttgctg tttattattg taatttgcct ccaacatacc tatttctttt ttctggattt
     6541 ttgtgtaaat ttccttatac tcgctttcac ttaacccctc ttttgctctt tgaatatcgt
     6601 cagctttttc tgcatagtta tcatcatcta tagacttaac tataatctcc ttaatttggt
     6661 caaatttagt tcttctttct tcatcaacac ctgccaaatt ataagtcatt ttatctaaag
     6721 cattttttct atcttcactt agctgttctc caagtacaaa tctcatacca tcagaatcca
     6781 atttaaaatc tgaaaaatca accatcctgt tagtccattg gtattcctct tctttagctg
     6841 aaagattttc cttcaaagat tttgcactgt caaatgcccc tagacaagct tgaccaacac
     6901 cactaaatgc agtcagtaaa ctcaatgcgc tttgtattcc agaaagaatt aactgtgttt
     6961 tcatttgttc ctcaagtttt gcctgtttct tttttagttc ctctatgtct tcttgctcct
     7021 cttctttcat ctttataatt tcttgaatca acaggttcat ctttgcatta acagaaagca
     7081 ttttattctc aagttcatct tttaaaactt ctatttgttg atttccagat tcaatttgag
     7141 ttaggatact ttccttataa gtattgaaat atccttcttt taactcatct ttctttcctt
     7201 gttccaattc tcttaaggtc ttgtccagat aatctgtcaa agaacgaata tctgtaacac
     7261 gatttcctcc aatttcactt tcttcaatca tctgcacata gaatgctgtt ttttgttcaa
     7321 gtgcaaataa aatcatactc cattcgctaa aattcttctt ttctcctata aaagattcta
     7381 ttctatcaga ctgcatcaat ttatttataa ggtattcata gatttccttt gcctccttat
     7441 aaaattcttt ctttatattc atatcatcca gagaaagcac tgctttattt ttcttctcaa
     7501 aactatagta cttctgaagt atttctctat ttaattgcat ctctattttc tcaactgcat
     7561 gaaaaatttc ataaatatgt gaaagttcta aaatatcagc ttctaaaatt cccactgcac
     7621 acaacagtct aagatttcta taacctttaa ttccagaaaa accaaatctc tcgaaaaatt
     7681 catagaagaa ggtatctatt gacaaagaat actcctgctt taacaattcc ttttctttta
     7741 atgcatctcc agagcgaccg tcttttccct ctgctccgac taaacctgaa tcttcacgtt
     7801 tgctaattgt ccagtcctta tgtttttcat aaagagcatt taatttctct ccatcatctg
     7861 ctacttggtt ctcatttgaa gcatgattaa acacatatct atcaaaataa tagtaatttc
     7921 tatcactgtc atctattgga gcagaggtac ctatatatct tattgtttct tcatatatac
     7981 ctctaattcc accaagacct cctgcaccac cagctcctcc agagcctcct tttcctgcta
     8041 gaccatgatt tccatctttt ttacttatag caccttttaa taaagaagct tttccaagga
     8101 gataaacctg tccagacttg ctcccaagac ctgcatttcc accattacct cctgtgcctc
     8161 cagttccacc ttgttttcct ttttctgctt ttaccacata gtattttgta cctttaacct
     8221 tagcaaattc ttttgactct tttttagaac ctattttagc attttcccca gcttctccag
     8281 ttatccctgt atctcctctt tttccatctt gtccatttcc accacgtcca ccatctacgt
     8341 aaagtttaag ttctcctctt ttttctattt ctttaaaaga taataaaata tcacctgaag
     8401 tacctgcatt attgccattt cttccattca ttccgtcgta aatagaacct tctccaacca
     8461 atttttctgc atccttacca gatacgttga ttgaataaat cccttctggt atacataatt
     8521 tttctgtaag tagtacaata ttcttacctg agaagacttc tttctccata ttacagtcaa
     8581 ttttcaactc acaatctcca tacatatata tagatgacaa aatattaagt tgataaccag
     8641 atattatttt agtcaaatcc gataaattca ctttaggtat aggaatatat atagaaagta
     8701 cattttcttt ctcatatgat tttatatatg tacaactttg aatcttctct atcctatact
     8761 ctgcatcttc aagctttttg gcttcttctt ccctatattc ttcttctgct atccaatatt
     8821 tgatatcttt taatgtatct tctaaagtat tttttagcaa tgattttaac aactcactta
     8881 gaccaacaat atgagtctca ctacagtagc tatttaactc taaaagaaga cgtgtttctt
     8941 ctaatatatt cactttctgt acgccatcac catatgaaag ttcttgtaca aactccataa
     9001 gctctttcat attattagtc ctactacata aatctgtata catcttatct aattgagtct
     9061 ttttatcctt tagactattt atagaagata gaatggaaat agaatcttcc ataccttttt
     9121 tgacactttc tatataagga actaaaagtt tttctaggtc tttataggtt acatcaacgg
     9181 ctttttgtat ttctatttta caagaagcag taagcatcaa tcccaaatct gttttttcaa
     9241 atgtttcagg tagatttttc atttcatcca acaacttttc tggctgtcta tctagtggat
     9301 tacataattt aatagataca ccatctgata atgctacatt taataagtac actaattcct
     9361 tacaaccgtc gtaatctttc tcttgactat ctaggtcttc ttttgctttt tccaatatat
     9421 tccttatttt ttcttctgtt acattaccga ttagtgtttt aggtatttta ttgataagaa
     9481 agaagaaatt ttctttatac tttgcataat caccaagcat atctttcaaa tttttgcctg
     9541 tttctatcaa taatgtatgt ctagagtcca gtaaggattg atagtctaaa ataactacca
     9601 tccctttcac ctttgaagat gtagaaatag tttcccttac aagcatagca ccagcaatca
     9661 tatactctgc tccacggtta tcaaaaaaac ctgggcaatc acataatgaa aaatttctat
     9721 ttgcaatatc tacaacttct ggacataaag tcttagaata tacaccattt ccaatttcga
     9781 cagctgaatc ttttgcagtt atatatattt gtccagtctc ttcatccgtt ttttcttcca
     9841 tcacgcaacc ttttaaataa tttatacaag tactctttcc aacgcctgaa gcacctaaga
     9901 ataacaaaat tgacttttct tttaagtcat attttctaag ctcgtctctt ccttcttcca
     9961 aaaggtttac gatttcacta taaagtttat ttgccataca tccatccctt tctatatttc
    10021 ggaacaatag ccttgatatt tttctttaat aagattctct tctacctttg aaacagctgt
    10081 tgataactta gtatttggag cattaaatcc tccgtcgtct cctctatctc catattcagc
    10141 gtattctctt gctggtcctg ttttgccttc attccaactt ccgccactaa attctatatg
    10201 atatttaatt atactatcta acttataatc ccaacttgta aaatatcctg tatagtaaac
    10261 agtcttatct ataccacttg gagaaaatga aagtgtatta tatccgtgat agccaccact
    10321 tcctccatat ccacctgaac ctggtctacc atttggacct gcttctccat ctttcactgt
    10381 tatatcacaa tgattaatta ttctattttt ttgttcctct gagtctgaaa tgaagtaaat
    10441 attaccttta ttaccagcat ttccacctct tccggcattt ccaccagctc cacctttgtc
    10501 acctttttta ccacttgtac cttttgcaaa atacccttta aacatctgtc catggaaact
    10561 acttgtatat cctgcatcag ctccattact tccagaatct cctgtttcac catttttacc
    10621 atctattcct ttagaaccat ttcctccatt tgcaatcagt gttaaacgat aatttctaat
    10681 ttcttcttta atatataagt aaatattacc tgcatctcta ccatcttcac catttttccc
    10741 atacatagaa ctatctacaa gttctccgct ctttccactt acattaattt ttacattact
    10801 acctacatca agattgttac aagcaagaat aatatttttc tctgatgcaa agtccattcc
    10861 aaggttatca tccatataaa tagtatcttt ggcaaagaac ataaatgtag aaacattgtc
    10921 atctactttt ctatttttga aatattccaa tacactacta attactaagt ttggaattaa
    10981 tgctcttatt tcaatacctt tcttatcatt aatattaatt gtttttacta taaatggatt
    11041 taaaatgtat ttaccaataa attctctaat tatctcatta ccttcttctc catatgcact
    11101 taatcttgtt atactgtctt ctaactcttc attttgtgta actgtaacat ttgtcaaaat
    11161 tgaattaaaa tcacttacac tatatacata atccttttta tttgcaactt ccattaattt
    11221 ttctctattt ggtaatgtaa atacctgttt ttgtacttca taagaaacta aaactttttc
    11281 taaattcttt cttattataa tgaagttttg ttccattatc ttcttacttg ccttttctat
    11341 aataaattcc atatgtacag aaggtaataa aatttcccca gttccatgtt ctgtatattt
    11401 acttaaagca tcaattatat ctaagtttcc ttttatctta ttttgtaatt ccatgcaact
    11461 atcttgtaag aataagccac tttgtttggc taatacgttg ataaatctct ctatagtaag
    11521 agttgttgta ctttccaacc agtttttaaa tacttttaca acctcatcat attttgctat
    11581 ttcttgaatc attgttatac ttccaataat actgataaaa tcttctgtat acgccttaac
    11641 ataatcttct aggctagata cgatattgtt ttgaatttct tttatcaacg tatctaaaac
    11701 taatcttgca tctgcactaa ttggatattg gaatgctgat gtcttatctt taacttcatc
    11761 taattctagt acttgcttta ataagccttc cctctcgtct gattcaagtg gcttacaaag
    11821 atgaatattt ttaccattat cttttagcaa ttcttcaaat aaccaagctt tttcctctat
    11881 attactatct aaattttcac ataattgttc atattttctt tttataacat tatatatttg
    11941 tttttctgtt cttcctaaga aactttttga aatgaaaaaa gttatactgc ccctaaaatt
    12001 gtctttatga agacacatat ttaactgttg aaacaggtct agtacttttg taagtctaga
    12061 atctaatata tctgcttctg gtctgaacct cccatgacta aagtcacagg gttcctgctt
    12121 cacagaattt tgcatactaa aatatgtctt actaattctc cacaggccat ccccgtagtt
    12181 cctacggttc ttacataagt tatttatttt gaattgttat tagtcttaat ccttctttta
    12241 atatatttat acttgcattt atatctctat cgtgagtttc attacagcta ggacaaatcc
    12301 attctcttat acttaagtcc ttcacttcct cgtttttata cccacattta ttgcatattt
    12361 gtgaacttgc aaagaattta cctaccttta caatttgtct accatgccaa ttagccttgt
    12421 attttaattg acgaataaat tctgaccatg atacatcaga aattaagcga gatagtttac
    12481 gatttttaat catattcttt acttgtaaat cttctatgca aataatatca ttctctttta
    12541 ttaatttagt agatagtttt tgtagaaaat catttctttg attggctata tgttcttgaa
    12601 gtcttgcaac ttttaatctt gctttatttc tatttaaact accgattgtt tttctcgata
    12661 attctctttg taatttagca agtttgttta atgacttctt aagatactta ggattttcta
    12721 taaagtctcc acaacttgag atacaaaatt ctttaattcc taaatctaaa cctatctgat
    12781 tattagtatt ttcaaatgct tcaatatcta catcagtaca gcataatgat acataatatc
    12841 taccacttgg ttctttagat atagtagcat taagtattct cccttgaggt acttgtttat
    12901 cccttatctt aaccatacca agtttaggta gttttatatg ttgactaaag tacataatgt
    12961 ttccatttgt aaagttagtt ttatatgaaa atctattagt tttctttgat ttaaatttag
    13021 gaaatcctgc tttttcttta aagaattttt tatatgcatt atctaaatct tttaaggcgt
    13081 tttgtagtga aaatttgtca ggttctttaa gccactttaa ttcttttt
//
LOCUS       DAEPLF010000087        13026 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEPLF010000087 DAEPLF010000000
VERSION     DAEPLF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.087"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..1290
                     /locus_tag="KR314_002127"
     CDS             <1..1290
                     /locus_tag="KR314_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0328510.1"
                     /translation="YFLFLDDALIAKVNFLKARKYADLLKDMQVSSLCNIKIGECECS
                     FENYECSIEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVAINNLAKSEVY
                     LEKIKNQSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILNMIKNIEEYTI
                     YDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKNIYLCFEKIQN
                     YEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIETEINNMRKVKLDLDKKKYVD
                     HLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDIALKNITIIIK
                     KYLKKDMFMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNLTVSIGIDTCK
                     NSSIDIREIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            1317..3203
                     /locus_tag="KR314_002128"
     CDS             1317..3203
                     /locus_tag="KR314_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0328511.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKEDSDIISKIYIYLT
                     LSAISIRKYDKANNFYFSAKKVIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSKNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELRKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            3211..5211
                     /locus_tag="KR314_002129"
     CDS             3211..5211
                     /locus_tag="KR314_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0328512.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTREFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            5473..6732
                     /locus_tag="KR314_002130"
     CDS             5473..6732
                     /locus_tag="KR314_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0328513.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVEEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            6793..8580
                     /gene="iorA"
                     /locus_tag="KR314_002131"
     CDS             6793..8580
                     /gene="iorA"
                     /locus_tag="KR314_002131"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBH0328514.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEMNDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            8582..9157
                     /locus_tag="KR314_002132"
     CDS             8582..9157
                     /locus_tag="KR314_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBH0328515.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            9210..10280
                     /gene="buk"
                     /locus_tag="KR314_002133"
     CDS             9210..10280
                     /gene="buk"
                     /locus_tag="KR314_002133"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBH0328516.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            10498..11199
                     /locus_tag="KR314_002134"
     CDS             10498..11199
                     /locus_tag="KR314_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328517.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKRSN
                     EHKCKSSIVIKNEQN"
     gene            11258..11764
                     /locus_tag="KR314_002135"
     CDS             11258..11764
                     /locus_tag="KR314_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBH0328518.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEMYELLYE
                     IDNNKLNI"
     gene            11939..12961
                     /locus_tag="KR314_002136"
     CDS             11939..12961
                     /locus_tag="KR314_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH0328519.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
BASE COUNT         5158 a         1227 c         2446 g         4195 t
ORIGIN      
        1 tattttttgt ttttggatga tgctctaatt gctaaagtaa attttttaaa agcaagaaaa
       61 tatgcagatt tactaaagga tatgcaagta tcttcactat gtaatataaa gataggtgag
      121 tgtgaatgtt catttgaaaa ttatgaatgt tccatagaat atttcaatga agtaataagc
      181 aataaaaagt atagaaatgt aaatataatt caaaaatata gagcatcaaa caacataact
      241 aaaatattaa taaagactag aaattatcct gtagcaataa ataatttagc aaaatctgaa
      301 gtatatctag aaaaaattaa aaatcagagc ttaaaagaag tagagaagtt tgatttattt
      361 ataagtttag ctatgtatta tgcaaaaagt aatgaaaaat cttttgaaca atcaatatgc
      421 tatttagata gagctaaaaa catcttaaat atgataaaaa atatagaaga atatacaata
      481 tatgatttag aaataattta tcatcaggca aagatatatt atatctttgg atattatgac
      541 aatgccttaa acacaaataa aaaactcctt gaaaaatcta aaaatgctaa aaattataat
      601 tatgtgaagt tagcatacaa gaatatatat ttgtgttttg aaaagattca aaattatgaa
      661 atgagtatga aatactttaa aatgtattat gagttaaaaa tgatgtatat aaaagctcga
      721 aatagaaatt atatagattc cttaaattat agacatgagt atatagaaac agaaataaat
      781 aacatgagaa aagtaaaact tgatttagat aaaaaaaaat atgttgacca tttgacaagt
      841 gcatataata gaactttttt gaacgatttt ttagaaaaac aagaagttag tgaatattgc
      901 acagcattta tgatagatgt agattacttt aaagaataca atgatactta tggacactat
      961 aatggagata ttgctcttaa aaatataact ataataataa aaaaatatct aaaaaaagac
     1021 atgtttatga taaggtatgg tggagaagaa tttctgatat tatctgtatg taaagattat
     1081 aaaaaaagca aaatatttgg aagaaaatta tgtaggattg taaagaaatt tttaaacaat
     1141 aatttgacag ttagtattgg cattgataca tgtaaaaata gtagtataga tataagagaa
     1201 ataattgaaa atgctgataa agctttatat aaagcaaagg aaagtggtag aaatagatgt
     1261 ttgcattatc atgattttaa gaatttttaa ctgttgaaat aggattggtg atttacttga
     1321 aaaggagttt aatagaagat ttaaagagaa atataaacaa gatagaatac gatatgttga
     1381 ctaaattcta tctaaatttg acagatttaa actacatctt ggattatcac aaagaccttt
     1441 taaatacaaa actagaagat aatagtagag tctatacttt atatttaatt agccttataa
     1501 actatataaa aaaagataga gaagcatcat acaactattt gatagagtca ttaaagtata
     1561 ttaaaaaatt gtcattaaaa gaagattcag atataatatc taaaatttat atttatctta
     1621 ctttatcagc tatttctata agaaaatatg ataaggcaaa taatttttat ttctcagcaa
     1681 aaaaagttat aagacaaaat catttaaatg agttgctagt cttgttaaat atgagtttat
     1741 gtgtagaaat aagtgcttta tatcagacca aaacggatgt aatagcttta gtagaagaag
     1801 tgtctttttt taaaacttta aaaaataaag ctcttgtaag tagggcaaat tatatatttg
     1861 ggagagttta tctatattta tttaataact ttataaaaag tatggagtac ctaattgaat
     1921 gtctaaaact ggctcaagaa aataatttat attctataga ggctttttgt aaatgtataa
     1981 taagtttgtg ttatttagag agtggaaata atgtagaagc tataaaatac ataaataatg
     2041 tattagatgt tgcgcacagt aagaactcaa tatcagtaac tgaaagagtg tctataaaga
     2101 tagaccttat atgggcatat ttaaaagaag atatgaataa agaagcagaa gttattttat
     2161 taaagactat aaaattaatg aaagttgttg aaggtgtata taaggattat ttatattcat
     2221 ttatattttt atattcagct gaaattgagt taaggaaaga taattgtagt tttgaaaagg
     2281 taaataccta tttaatttta tcaaaaaata tatataaaag ttttggcgat gtttttatat
     2341 ataattctaa actagattac atatataagt tgtatggaga tttatatata aaatttggta
     2401 atatagatga aggtataagt tgttatataa aaggtttaaa tttagttaaa aagagttcat
     2461 tgaatcttag aaaaatatct atattttatg ggctaatagc aaatggatat gagctaaaaa
     2521 aagattttaa actggcaata gaatattata aaagtatgga ttggtattct gataggtggg
     2581 aaaaaaataa ttcctataaa acatctcaag caattcacaa acaatatgaa ctcagacaaa
     2641 aacaagagag tttaagatta ctagtggatg ataataagaa attagaaaat gatattttaa
     2701 aagatggact tactaaacta tataatagaa gatatttgga acagcagttg gatttattta
     2761 atggaaccag agacaaaaat atggttgcaa tactagtcga tatagattgt tttaaaagtt
     2821 ataatgataa ttatggtcat ctagcaggag ataaagttat agttaaggta actgatatta
     2881 taagaagtgt atttatagat attaccgaac atataatcag atatggggga gaagaaattt
     2941 taatactagt agattctata gaatcaatcg gtgttgaaga gattgataat atagaattat
     3001 atattaaaag agttttcaaa cttcttgaat ttgagggtat tgaacatata tattctaaaa
     3061 taaaaaacta tataactata agtgctggag taagtataaa aagatgtcgt agtagaaaag
     3121 atgttgaaat attaattgaa gatgcagata agaacctata taaatcaaaa aaagcaggta
     3181 gaaatcaata cattataatt taaaattgag gtgattttgt tgcgaaaaaa aataggaatt
     3241 atagcctctg atattgaatt aaaagaaaga atagaagaat tatatagaga agatgtagaa
     3301 aatggaacta taataatcga catacttaac ctagacttaa tggaaaatca aggtcgtata
     3361 cttgttgaaa aaggtgccca agctataatt ggtagaggtg gaggttatag tttagtaata
     3421 gatacagtaa atgtacctgt tataccaatg aatatgaagt caactgacct tttaagagca
     3481 atagaaatag caaagaaata ttctaaaaaa gtagtattga ttcttggaga caatgaagtt
     3541 tcttttgact atgttggctg gagaaatgtc ataagtactg aaataactga agaatggttt
     3601 gaatctaagt atgagataag aagtaaagtt gtaaaatata tagaccaaaa agatgaagtt
     3661 gtaatcgtag gaggaggttt ggcatgttct tttgcaaggc aatatggaat tgatagtgta
     3721 tttgcaactg ctagtgatga atctataaga gaagctgtag aatattgtaa aaaattattg
     3781 gatacattag gtgaagaaaa gtttaataat gaagtgttac gaaatatact tgatggtata
     3841 aaagatggag taattgctat agatagtaat ggtagtataa ttttgtacaa tgaaagtgca
     3901 aaaaacatgc ttaaagtaga gagaaaatgt gcacttaata aatacatctt agatgtattt
     3961 cctaaaatgg agtggatgtt agattgttta catgaaaaag aggatgtaga agatagaaag
     4021 ataagaaata taaataatct aattgttaat actaggacaa cattaataaa agttgataat
     4081 agtacttatg gagttttagg aataatacaa gatataacca agctacaaaa cttagaaaga
     4141 aaaataagat ttgacttgaa tcaaaaaggt ctctatgcta gatatacttt tgatgatttt
     4201 ttatttaaag ataaattaac tagagaattt attgaagaag ctaaaaaaat tggaaaaagt
     4261 gattatacaa ctttacttta tggagaaagt gggtctggta aagaaattat agctcatagt
     4321 atacacaata taagtaaaag aaaagatagg ccttttgttg ctataaattg tgctactata
     4381 gcagaaaatt tacttgaaag tgaacttttt ggctatgaag aaggtgcatt tactggtgct
     4441 agaaagggtg gaaaaagagg actatttgaa cttgctcatg gagggactct ttttttagat
     4501 gaaataaata gtctatcctt taatattcag acaaaactat taagagtaat agaggaacgt
     4561 caaataatga gaattggttc tgattatata atacctctgg atataaggat tatagcagca
     4621 acgaacgaat ctttaaccga aaaaatagtt atgggtacat ttagagcaga cttattttat
     4681 agattgagta gtttagagat aaatatacct cctttaagag acagaagaga agatataata
     4741 cctttgttta acaattttgt aaatgaagta ttaaaggatg atggtctcaa tggaataaac
     4801 agtatagatg aaaattttgt tttgactaaa gatgaaatgg ataagctata taattatagc
     4861 tggcctggaa atgtaagaga gttaaagaca atagctcaaa aatatgttgt gacaggtaaa
     4921 attaaattga gacaagatag agattttaaa actaaaaaat tacttcctaa ctctgaagta
     4981 gacaaattta attctgaaac gacagcaagt gtagaagtcc aagatgaatc tataaatata
     5041 agtaagataa atgatggtaa gataagtata gatataaaag aagtaaataa gtatgttgaa
     5101 gaaaaaataa taagtatgct ttttgctcag ggactttcta aaaatgaagt tgcacaagtt
     5161 cttggaataa gcaggacatc tttatggaag aaatacaata aaaatattta aatataatta
     5221 catataaatt attttgaagt aatgttaatt ttttaaaaca agtgttaaga aaaattaaca
     5281 ttactttttt gtaaattttt atttttttag acaggcatat atatttataa gaaaaatagt
     5341 taaaacactt aatttaataa tgaaaaaaat aataattcta aacaaaatca attttacata
     5401 aagtgggatt tattggcata gtaattgctt aatatataat gtaagagtaa atacaatttt
     5461 aggggtgatg gtatgagtca aagtatggtt ggtaagcatg caatgtggcc aaaagaaaat
     5521 gatgttatat ttagcatatc tggtagagct caagcagcag agaaggcttt tggtatggat
     5581 aatgtaataa atgcaacaat aggcgctcta atggatgatt caggaaaatt aataacaatg
     5641 aaaactgtat atgaagaata taaagcatta gataactgtg agataggtgc atatgcagct
     5701 cttgaaggtc aaccagacta cttagaagca gttaaaaaag tatttttcag agattactta
     5761 ccagaaggac atataagagt attagcttca ccaggaggaa gtggtgcaat aaaattagct
     5821 gtatggaact atactaatga aggagatgag gtattaacat ctgattggtt ttggagtcca
     5881 tatgtaagta tagctgaaga ggctaataga aaagtagtta attaccaact ttttgatgaa
     5941 aatagaaggt ttaattttga gtcgttcaaa gaaaaatttg taaatatagc agaaaaacaa
     6001 ggtagagtct ttacaattat aaacacacca gcgcataatc caactggata tagtgttgca
     6061 gatgatgagt gggataaaat attagactta tcaaaagaag tcgcaaaaga taaagacaaa
     6121 aagataatat ttttcgtaga ttcagcatat attgactttg caggtgatga tgatgtatgt
     6181 agaaagtttt ttaagaaatt ttctaatttg cctgaaaacg ttctcgtatt ggttggattc
     6241 agtatgtcaa aaggatttac agcttatggt atgagaatgg gggctataat ttgtatatct
     6301 tcaagtgaag atgtagctga agagttccat tactcatgtg ttcattcatg tagagcaaat
     6361 tggtcaaatt gtaatagaag tgccatggca gtattatcta acattgtaaa tgaccctaaa
     6421 aaattcaaag aatatgagga tgaaaaagaa atatacaaaa acatgcttac tagaagagca
     6481 gatgtatttg tagaagaagc tgaaagagta ggattagaaa ttcttccata tatagcaggt
     6541 ttctttgtaa gtataccatg tgataatcca aaagaagtat gtgaagaatt gactaaacat
     6601 aatttatttg cagttccatt aaaaatgggc ttaagatttg cagtttgtgc agtttctgaa
     6661 gataaatgta agaaagcacc aagtattata aaagaagcac ttgaaagttt agaagtaaaa
     6721 ataaataatt aatttcattg aacaaattgg gtggactatg aatttaataa ttgtagatgg
     6781 agggaatttt atatgaaaca attgatgaca ggtaatgaag caattgcacg tggagcctat
     6841 gaggctggtg ttaaatatgc atctgcatat ccagggactc caagtacaga aatattagaa
     6901 aatatagcaa catataaaga tgcaatagtt gcagagtggg ctccaaacga aaaggtagca
     6961 cttgaggctg ctataggggg ttcaattgca ggagcaagaa caatggcttc tatgaaacat
     7021 gttggtttga atgttgcagc tgatccaata tttacttatg catatacagg ggtaaatggt
     7081 ggtatggtac ttattacagc agatgagccg ggaatgcatt catctcaaaa tgagcaagat
     7141 aataggatgt atgcaaaatt tgcaaagata cctttatttg aaccatcaac tagtcaagaa
     7201 gctaaagata tgataaaaga agcttttgaa gttagtgaaa aatacgatac accagtttta
     7261 tatagagtta caacaagact ttgtcattca aaaggattag tggagtgcta tgatagagaa
     7321 gaagttgaaa ttaaagagta tgttaaaaat gctaagaaaa tggtaactgt tccagcaaat
     7381 gctcaaataa gaaggggcgt tgttgaagaa agaatggaaa tccttaaaaa attctcaaat
     7441 gaaactgatt taaactacta tgaaatgaat gatacaaaaa taggtgttat agcttctggt
     7501 atgtgttgta attttgccaa agaagttttt ggaaaaaatg catcatatat gaaactagga
     7561 tttacaaatc cattacctta tgaaaaaata aaagaatttg cagaaaaagt tgataaaata
     7621 tatgttatag aagaaaatga cccatttata gaagaacaaa taaaagcata tggaatagat
     7681 tgtattggaa aagatgtaat accaccatat ggagaaatga caccagatgt tttaagaaaa
     7741 gctatatttg gaaaaacaaa tgatactata gaatacaaat ctgaattagt aacaccaaga
     7801 ccaccaagtt tctgtgcagg atgtccacat agaggattct tctatgaact tggaaagaga
     7861 aaaaatttaa tagttggtgg agatataggt tgttatactc ttggatttgc tcctccatat
     7921 aatggaatag attatgttgt ttgtatgggt tctgcttttg gaactgcaca tggagctcaa
     7981 aaagttctta atatgaagga tgataatgaa aaaagattgg taggtgtact tggagactca
     8041 acattcttcc atacaggaat aaatggactt ttagatgttg tttacaatag aggtaattct
     8101 atttcggtta tattagataa tagaataact ggaatgacag gtcaccaaga aaacccaggt
     8161 tcagggtata ctttacaagg tgctaaaact aaagaagttg atatagaagg attagttaaa
     8221 gcttgtggaa tagaacatgt aagagttata aaccctaaca atttaaaaga agtaaatgaa
     8281 gcactagatt gggctttagc tatagaagat gaaccatcag taataataac aagatggcca
     8341 tgtgtactta aaaaattctc taaagaagat atagaagaat ttaataatcc atttaagact
     8401 aagtgtaaag ttgaccatga taaatgtata ggatgtaaat tatgtttgaa aactggatgt
     8461 ccagctctat catttgataa agagaataag ttatcaaata tagatagaaa tcagtgtgta
     8521 ggatgtggag tatgtgcaca agtttgtcca aaacaagcaa tagttaagga ggaaaaataa
     8581 tatgactaag agtatacttt tggtaggtgt aggaggtcaa ggaactatac ttgctagtaa
     8641 attattaact atgggattga tggaagctgg atatgatgta aagatgagtg aaatacatgg
     8701 aatgagtcaa agaggaggtt cagtttcctc acaagttaga tatggagatt gtgttcattc
     8761 tccagttata gaaattggtg gagcagatat attagtatct tttgaaaaaa tggaagcttt
     8821 aagatggttc aattatttaa aaccagaagg aaaagcttta gtaaataatc ataggataga
     8881 ttctatgact gttttaatcg gaggagctga atatcaagaa agtgatatag atgcagagtt
     8941 aagtagatta aatgcaaaag ttataaatgc agctgataaa gctgaagaac taggaaattc
     9001 aaaaataatg aatgttattt tattaggatg tctagttaaa tctatgcaat tagagtctat
     9061 tgattgggaa aaaattataa gtgaaaatgt taaaccaaaa tttgttgaat taaacataaa
     9121 agctttccat gaaggtatgg aaatggttag caaatagttt ataatagata taaatagact
     9181 atagaaatta aggtgaaagg aagaaaatta tgacttacag aatattagcc ataaatccag
     9241 gttctacttc tacaaaaata gcagtatatg atggagaaga acaaattctt gtgaagacga
     9301 tagaccatcc agctgaagag attgcaaaat ataatactat acaagaccag tttgaaatgc
     9361 gtaaggaagc agttttgaat attcttaaag aaaatagtat agacttaaaa tctcttagtg
     9421 caatagtagg aagaggtgga gttttaccac cagtaaaatc aggagcatat ctagtaaatg
     9481 aagaaatgat tgatgtacta agacatagac cagtacttga acacgcttcc aatttaggtg
     9541 ctgttgtggc acatgcaata tcagaacctc ttggaatcaa ctcatatatt tatgattctg
     9601 ttgcagtaga tgagcttata gatgtagcga gaatatctgg actttgtgga atggatagat
     9661 caagtgcagg gcatgcatta aatactagag caatggcttt aaaatatgct aaggataaag
     9721 gaaaagatta taagagctta aacttaatag tagctcacat tggtggagga gtaagtattt
     9781 atcttcatga aaaaggaaga atggttgata tgctatctga tgatgaagga ccattttctc
     9841 cagaaaggtc aggaagagta cctgctacaa aattagtggc tgcctgttat tcaggtcaat
     9901 attcagaaag agaaatgact aaaaagataa gaggtaaagg tggtatagtt tcatacctaa
     9961 atactgtaga tgctagagaa gttgaaaaaa tgatagcaga aggaaatgaa gaagcaaaaa
    10021 ttatttatga agcaatggct tatcagttag caaaaggtat tggagagtta gcaactgtag
    10081 tagatggaaa ggtagatgct ataattataa caggtggaat tgcatattct gaaatgttta
    10141 cttcaatggt taaaaagaaa gttgagttta tagcaccagt agaaattatg gcaggagaaa
    10201 atgagttgga atcacttgct tttggaactt taagagtact aaatggagaa gaagaagcta
    10261 gaatttatag tgaaaattaa taagaaaaat taaaaatgtt tatatactaa aaaagtggta
    10321 tatccgtttt ttatacacga aagcttagag tataggtgaa aatgtataaa atttggattg
    10381 ccacttttta taattaaaat tttatcttaa tctaataaat aattctgtaa aatttcgtat
    10441 ttatatatat attttattgg tatttattgt tattattatt atgaggagtg atttttgatg
    10501 ggaaaaataa gtttaaaaag tatgataaga ttattttcag gtttttttat atttgcaatt
    10561 tcttctgtgc ttatgattaa tgcacatgta ggtcttatgc cttgggatgt acttcaccaa
    10621 ggtttatcta taaagctagg cataactata ggtcaggctt ctataatggt aggagttgta
    10681 attgttatat tggatgcagt ttttggtgaa aatataggat ggggtacttt actcaacatg
    10741 acatttatag gaatttttat tgatttagta atattttctg gagtgatacc tcatgcaagc
    10801 aacacttata taggtgtgtt tatggttgtt ataggaatta ttttggcagc tatagcaagt
    10861 tttctatact taggtgtttg tttaggaagt ggaccgagag atgggcttat gattgctctt
    10921 cagaaaaaaa ctaataaatc tgtacgtctt gttagaacaa ttcttgaaat actagcatta
    10981 gttgttgggt ggttattagg aggttctgta ggtattggta cgcttgtaag tgctttaggt
    11041 ttaggatatg tattacaaat agtgtttaga atatttaaat ttgacacaaa gttgctgaaa
    11101 catagattta ttatagatga tataagagaa tggaaggaaa agagaagcaa tgaacataaa
    11161 tgtaaatcaa gtattgtaat aaaaaatgag cagaattaat tatgctaaaa tatatttgta
    11221 gtttatctaa tttatagtaa agtcaggaga ttttgaaatg gaattatggg atttatataa
    11281 tgctgatgga attaaaacag gaaatgttat aaaaagaggt aattctattg aagaaggtta
    11341 ttaccatttg gcagttgaag tgtggatttt aaatagtaac tctcaaattt taattcagaa
    11401 aaggtctaaa agtaaaaaga cattgccaaa tatgtgggga atgacaactg gatgtattgt
    11461 ttctggggaa gatagtttag aaggagcaat tcgcgaagca aaggaagaaa tagggataga
    11521 tataactaaa gatgagatga aagtttttag aagtatgatt catgaagata ctctttggga
    11581 tgtatactta gttaaaaaag aatatgatat atccaaagct attttacagg aggaagaagt
    11641 aagtgatata aagtgggtaa gcacagatga aataagacaa ttactaaaag agggtttgtt
    11701 ttttgaatat ccagagatgt acgaattgct atatgagata gataataata aattaaatat
    11761 atgatgtaga gcagatttca gtataagtaa gattatttaa aatgtatctt ctctaaattt
    11821 taatattgtt ttatgaaaat taataagtta tttttagtat ttttctactt gttttttgag
    11881 attaaaataa aaagggcagt cattataaag tcataaaaat ttggggagga tttaaactat
    11941 ggaagataac aaaggattgg cacgtgatag ttcaaaatat aaaattgttt ctatatctgt
    12001 cgtagtaatt attctaatta cattttggta tatgttaaat atggtgcttt taacatttat
    12061 tatgactttt attttctata acttgcttgt agccacaaga aaacgaatta agaagttttc
    12121 ttctttaaat attccagatt cattgataat tatagtgtta tatgcactat ttgctatatt
    12181 gttagtattg attagctatg cagttgtacc aataattata gttcaactta cagagctgag
    12241 tagggttttt agtgattttg atgtaaatca atttgctcaa tctttaggac caaaattgta
    12301 tcctattgtc tcaaagctgg attttaataa atatatttca caagcagggc tacttatagc
    12361 atcaacagca acaaaagttg gaagttttgg agtaaatata ttattggcat ttttactgag
    12421 tttactcctt ttacttgaaa aaaatgaaat taaaaatttt ggagataaac tttctgatag
    12481 taagatttct tttatttata attcgcttgt attttttgga aagagctttg ttaaaaattt
    12541 tggtgaagta atgaaagtac aagttatgat tgcctttata aattctgttg tttctatgat
    12601 tttcttaggt ttcatgggat tccctcaaat atgggcttta ggatttatga tttttgtgtt
    12661 gggtcttatt ccagtagcag gagttatagt gtctttgatt ccattaactg taatagcatt
    12721 taatacagga ggaataacaa aggtttttgg agttttactt atgatttgta tagtgcatgc
    12781 agtagaaaca tacatattaa accctaagtt gatgtcgaat agaactaaac ttccagtttg
    12841 ttttgttttt attattttac ttgtaggaga acattattta ggtgtatggg gattactaat
    12901 tggagttcca atatttatgt tccttatgga tattctgggt gttaaattta cttctaggta
    12961 atatattaaa ataataattt atttttatat ataaattatg aattgtttta aaaaagaacc
    13021 tataaa
//
LOCUS       DAEPLF010000088        12988 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEPLF010000088 DAEPLF010000000
VERSION     DAEPLF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12988
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.088"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..1665)
                     /locus_tag="KR314_002137"
     CDS             complement(<1..1665)
                     /locus_tag="KR314_002137"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBH0328520.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNLENEKVREECYKVLEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(1862..2584)
                     /locus_tag="KR314_002138"
     CDS             complement(1862..2584)
                     /locus_tag="KR314_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0328521.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECIGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            2975..4402
                     /locus_tag="KR314_002139"
     CDS             2975..4402
                     /locus_tag="KR314_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0328522.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAVSAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGHEVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            4395..5174
                     /locus_tag="KR314_002140"
     CDS             4395..5174
                     /locus_tag="KR314_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBH0328523.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            5437..6162
                     /locus_tag="KR314_002141"
     CDS             5437..6162
                     /locus_tag="KR314_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0328524.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            6292..8217
                     /gene="mngA"
                     /locus_tag="KR314_002142"
     CDS             6292..8217
                     /gene="mngA"
                     /locus_tag="KR314_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBH0328525.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDSNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIVLAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKKADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVIGKPVAA
                     LNNGLINWLNGMTGINAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIHVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            8361..10976
                     /gene="mngB"
                     /locus_tag="KR314_002143"
     CDS             8361..10976
                     /gene="mngB"
                     /locus_tag="KR314_002143"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBH0328526.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNKDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YNRNEIVNAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKISDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIINVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGKKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKIPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANVVFNKNIKTAKWTNLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            11258..12412
                     /locus_tag="KR314_002144"
     CDS             11258..12412
                     /locus_tag="KR314_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBH0328527.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(12535..>12988)
                     /locus_tag="KR314_002145"
     CDS             complement(12535..>12988)
                     /locus_tag="KR314_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0328528.1"
                     /translation="VQNHLEMYFSESLFMKNIYLDSAKDYIKFMGNILYKNKYVKPNY
                     IDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVCLIVNDKPVKWGEEKVQIIAMIPI
                     NEKEKEKFNYIFESFIEILSEWNNVKELTKADNYSSFMNRIAYLIQNI"
BASE COUNT         4992 a         1536 c         2239 g         4221 t
ORIGIN      
        1 tttataatat atcattatac tttcgtaagg ttttatttcc attttatttt caattatatc
       61 tctagtgtaa ttggtttcaa gtattgtgta gtttaattta ttcaaataag tttcaggtaa
      121 tttatatact acattatcat gagtaaaatt acttataaca agaagatttt ggtttttata
      181 ctctctttca taaacaaata gatttttatg attttcatct aacaatctat aagtaccttc
      241 tgtaaaaata tcatattgtt tacgatattt gattaatctt tgataagtat aaaatactga
      301 acgagaatca ttaagctcat tttctacact aatattgtca ttattaactt ttaaccacgg
      361 tttatgatta gaaaacccct tataaatagt attatcccat tgcataggag tacgggcatt
      421 atctctggat ttttgttgaa gtataagcaa tgcctccttt tcatcaatac cagatttaat
      481 catatgatag tatgcattct tactttcagc atcatcatat tcatctattt tagtaaagta
      541 ggcatttgtc atacctattt cttcaccttg atatatgtat gtagtacctt gtagaccaaa
      601 tagtgttatg gctaacatct tagcactttc ttgacgaaaa aatctatcat ctccaaatcg
      661 agataaagct ctaggttggt cgtgattaga gtaaaataga gcattccatc cctttccttc
      721 atacatttta tattgaaagt cagattgtgc tttttttaat ttatccagag agaacatttc
      781 attagtccat tttgcgcctc ctttataatc tgttttcata tgatggaagc taaaaaccat
      841 agatagttct tctcgatttt ctctagtata gttgatacac tcttcaggtg tagtagatga
      901 catttcacct acagtaacta tatttttagg tttgaaaact ttttgattca tattatgtaa
      961 atattcgtgt atacgaggac catcagcgta atgagttctt ccatctcctt ttggccctgt
     1021 tatagggtca tcaggtaatc caggtgtttt ggagagtagg ttgactacat ctaaacgaaa
     1081 accatcaata cctttatcag cccaaaattc tagtactttg taacattctt cacgaacctt
     1141 ttcattttcc aaatttaaat cagcttgagt ttcttcaaat aaatgtaaat aatattggtt
     1201 agtagtttca tcatatttcc aagcagttcc actaaatctg gatatccagt tattaggaac
     1261 agaaccatca ggtttagcat ctttccagaa ataataatca cgatatggat tgtctttgct
     1321 ttttttagat tctttaaacc atttatgttc agtagatgta tggtttagga ccatatccat
     1381 cattattttt atgtctcttt tatgagcttc ttttaagagt ttttcaaagt cactcattgt
     1441 cccatatttt gggtctatat tataataatc ttctatatca tatccattat ctctttgagg
     1501 agaaacgtac ataggagtca accataataa atcaacacct aaagaataca gataatctaa
     1561 tttttctata ataccattta tatcacctat accatcattg ttgctatctt tgaaactttt
     1621 tggatatatt tgatagacag ttgctttttt ccaccaattt ttcataattg atacctcctg
     1681 taatttataa gttaattata atatgtttaa acaaattgtc aagtattaaa ctaatattaa
     1741 agctaaaata tacaataaat aattatttgt ataaacatat aattgtatat agaatagtga
     1801 ggttagtatg ggataaagtg tctaaacgta aaaaaattat gtttaattaa acataatttt
     1861 tttaatatct ttgagtaaca tccatgaata caaatgtttc aggtgtatgc tttgattctg
     1921 tatattcaaa gagctttcca tcatcagtat atacactatt tttaataaca ccaacacaat
     1981 tataatcatt tagaggtagc gtttttaact caagctctgt agctttttca attttaaata
     2041 tctttctgga tgaaacaatt tttgtaccta tacactcttc tatatattta taaatagagc
     2101 cttgtgctat agaaacatca aggtctttaa ctatactaca tagaaaatag ttaatgtcga
     2161 gaattttagg tatgttatca atatatctta gtctgtgaag gtaatatact tcactaccaa
     2221 ctttaaaacc tgtcttttta gataacttat tatcaattaa gattttgctg aagtggagaa
     2281 cactagtagc agtattaacc tttttatctt ctacaatcga ttctacacct ttgaaattgt
     2341 gcatatttag aaaaaaatca ttagaacaac tattttctaa cactacaaca cccttgcctt
     2401 ttatactttg aacatatcct tctgtgttta attgagaaat tgctctacgt acagtgtttc
     2461 tgctacatga aaacctttca ataagttgaa gttcagtagg cagcatcata ttacttgtat
     2521 atttaccatc aagtatttcc tctttcaata cttcatatat ctctgaaaat ttatttttag
     2581 ccatataccc ctatccctcc ttaaaggtct ttagtaatat tattgaactg atatgaacaa
     2641 attatatcaa atgtgtttag acatgtatag tgaaaataga attatataaa ccataaaaag
     2701 gataaatttg aactattttg tgggttgttt caaatacatg gttaaacata ttttaaaaat
     2761 aaaaaaaata aatttaaaaa ataggctaga tatagcaata tacttaaaag gatttagata
     2821 tgaaactaaa tgataaaaat aaaatgaata aaaaatattg acaatttgtt taaacaaata
     2881 taatatttaa aatataaaat gtgaataaat cgttttcaca aaaaattaaa ccgaaataga
     2941 aaggggattg gatatgaaga ggttcttaaa caaaatgatg caacaactgt cacttttagg
     3001 aaagactatg ctggtgccaa tagctgtaat gccagcagca ggtatattag gattattatt
     3061 tggtccaaat atgcttaatg tgccagctat atctaatata tctaatatag tattttcaaa
     3121 tgtggactac ttatttttat taggagcagt tagtgcttat tcaaagtcta aagacaagac
     3181 aagtgtaatg gttgcagccg tagttgcata tatgatattt aagtcaaatt tagagttatt
     3241 gaatgataca ttaaatgcag gtgtatttgg tgggataatt gttggagctt gtatagcatg
     3301 gacttacaat aaaacatata aagttaaagt tccagcattt ttaagcttct ttgcaggaga
     3361 aaaatgtgta attacactat caccagtggt tgctttaatt ttatcatata tatttagttt
     3421 attatggata tatccacaag ctgcaatgaa tcaatttggt ttttggttgg gaggagctgg
     3481 tgcaatagga atattcttgt ttttattctg taaccgtgca ctaataccaa ctgggttaca
     3541 tcaagtttta aatgcttaca tattatttga aatgggtgaa tatacaacat caacaggaga
     3601 agttataaga ggagaaattc ctagatttat ggcaggagac ccaaatgcag gattcttctg
     3661 gacaggattt tacataatta tgatgttttc aatacctgct atttcttatg ctatatataa
     3721 aacagcacaa gataaaaaga agcaagaagt aaaaggtgca atgaccgctg gagcattgac
     3781 ttcttttatg gcaacagtaa ctgaaccaat agaattttcg tttttatttg catctccaaa
     3841 gttatatata attcattctt tttattctgg tctaggtgga gttgtactat atttactggg
     3901 tgctcgcttg ggttcattta atggagcaag tataattgac tatgtcttaa gttttagtta
     3961 tggagataaa gcgtggttag ttatacctgt aggagttgta ttctttgtat tatattatgt
     4021 aacatttaaa tatataataa taaaagataa tgtacaaaca ccaggacatg aagttgagat
     4081 agagataggt gataaagtta gtgaaaaaga aaagaattta aaactttctc atggtaattt
     4141 ttcatatatg gctaagcaga taattaaaaa ttgtggtggt tatgaaaata tagtgactct
     4201 taataattgt atgacgaggt tacgtttaga agtgaaggat gccactattt tgaatgatga
     4261 taatattaaa aaaacaggag ccaaaggggt aattaaattg agtaatacat cagttcaaat
     4321 catcatagga acggatgttg ttaaagtaaa ggatgagatg gaaatgcaac ttgatgagtt
     4381 gagaaagcag gcagatgcat aaaatgaaga aattagtagt tagggcagat gatataggat
     4441 attcagaagg tgttacacta gggataatat cttcttataa gaacggtatt gttacaagtg
     4501 ctggaatcat ggtgaatatg cctttttcaa gacaagctat agaaatggta aaaaatgatg
     4561 atgtatgttt tggtttacat gttaatttag taataggaga accttgttgt aaagaacata
     4621 ttaagataaa gaatttgtta gatgaaggtg gaaaatttgt ttctagcaaa ataagaagaa
     4681 cgcaactact gacaggtaaa gaggttttta attatgaaga agtatatgaa gaagtttgtg
     4741 ctcaaattga aacttacaag ttgtatatga gtaaattgcc tgagtatatt gatatacatg
     4801 gtatagaaga agatgtatca atatcagcaa tgtgtgatgc tgcaaaagaa tacggtatac
     4861 caagttgtcc ctattattta gattcatgta ttagcatgcc aagtaaatat cctcagaatg
     4921 atttttacaa gttaaataaa tcttttgtta aattatttga agaaaaatat ataaatttag
     4981 ataaagatat aacattgatg gttactcatc caggatttat agattatagt cttataaaat
     5041 catcttctct tattcaagag agagtatatg aacatgcaat gctatgtgat aaggaagtta
     5101 aaatgtggtt aaaaagaaat aatattgaat tggttgattt taaagatatt ttcaagtcta
     5161 ataattatat ataaagaata gattacctaa agttttaatt aaaaataagt atttgaaaaa
     5221 aatgtgtatt ttctaaaaaa atattgatag agatgtatca aaataaaaac ctgaatcatc
     5281 tctatcaata taggaagtac acatttttta tgtataataa aaagagtagt atgcattaat
     5341 aaattaaaaa tattaaatta taattatatt aacgaaaatt ttagctaata tttttgaatt
     5401 ttacttatga ataataaaat agatggagga gaaactatgg aattttatga aagagcaaaa
     5461 aaatatgagt ataaatttac agatacagaa gacatgataa ttgagtatat gagaaaaaac
     5521 aaagaagaaa tagtaaaatt atcaatacaa tctttagcag ataaattttt tactgtccca
     5581 aataccatag ttaggctatg taaaaaaatg gagtatgatg gtttctctca actaaaaaac
     5641 aatttaaaag aagagttaaa aaatgaggat aaagagccaa aaaatggttt tgaatcagat
     5701 ataaaaaaga cattagagct aatagattat gaaaaaataa ataaagtagc agatataatt
     5761 caaaaatcta ggagtattat agtttatgga ataggtcaaa acattccttt atgtgagata
     5821 ttagtaaaag aaattagagg tgggcatgat aatataactt actatagcca aagacatgaa
     5881 gtacttcatg atataggaaa attaggtgaa caagatatgt ttttcgcgct tagtttaagt
     5941 ggagaaacga aagaagttat agaagcagca aagacagcag ttaataaggg ttgtaaggta
     6001 gtagctctaa cacatatatg tgaaaatcaa ttgcaacaaa ttgcagatat aagtttatat
     6061 tgctattcaa caattaaaca agtaaatggg tacaatatat cagataaggt tccaattatg
     6121 atgattatga gagcaataat agaacaatac tggttaagat agagatttgt gtataaatac
     6181 acaaatcctt attttttaag aagaaattgt gtgtataatt tcaaaaaaac attttcatga
     6241 tttattttat gctatattca taacattaaa tgatttaagg aggtcgtaat tttggaattt
     6301 aagaaaatca caaatgaaaa ccttgttttt ttaaaacaaa aaataaaaac aaaggaagac
     6361 gcaataaagt tcttagcaag aaagctatat gatgaaggca agttaacatc ttatgaggaa
     6421 tatttagaag ctgtaatgta cagagaaact ttatctccaa caggttttga aagagggctt
     6481 gcaattcctc atggaaaatc taagtcagtt aaagaagctg catttgcagc tatgacctta
     6541 gagacaccaa tagaaaattg ggaaagcgta gactcaaata ataaagtaga gctaatattt
     6601 ttaatagcag taccagaaag tgaaggtgga aatacacaca tacaaatact ttctgagtta
     6661 gttactagac tgtctaatga agattatatg aatatgttgt taaattcaaa aactaagaaa
     6721 gagttattta ataacttaga tataaaagaa aagaaagaac aaataaaaga agagattagg
     6781 attcaaaatg aatcaaaaaa aattgtatta gcaataacag catgccctgc aggtatagca
     6841 catacatata tgtcagcaga agcattaatt aaagctggca aagaaatagg tgtggatgtg
     6901 tatgtagaaa agcaaggagc taatggtatg gtggaccctc atacaccaga tataataaaa
     6961 aaggcagatg caataatata tgcaacagat gtagcaccta aaaatacaga aagatttgaa
     7021 catttgccaa acataaaaac gtcagtagca gctccactaa aaagggcaaa agaaataata
     7081 tacgaagctt tagaagtagc acaaaaacaa ggtaaaggtg attatataga aaaatcatct
     7141 gatatatcat attgtgaaaa atcatcatgg aaaaaagatg taaaatcagc aatacttaca
     7201 ggagtatctc atataatacc acttattgtg gcaggaggta tgatacttgc atttgcagta
     7261 ttagcatctc aagcatttgg tttacaagaa ctatacaatc aagaaggttc ttggttatgg
     7321 caattaagaa agctttctgg agggatgtta ggtacattga tggttccagt attatcagca
     7381 tatatgtctt actcactagc tgaaaaacca ggtcttggac caggatttgc agcaggtcta
     7441 gcagctgatt taataggtag tggatttctt ggaggtatgt taggaggttt acttgctggt
     7501 tatatagtaa aatttttaaa gaagaatata ccagccaaag gcacattagc tggattcgta
     7561 agtttttggg tatatccagt agttggttct ttggtagtag gtttattgat gttatttgta
     7621 ataggaaagc ctgtagcagc acttaacaat ggtcttataa attggttaaa tgggatgact
     7681 ggaataaatg caatactact aggtgcaata ataggagcta tggtttcatt tgacttaggt
     7741 ggaccaataa ataaagcagc ctatacattt gcaataggag caatagcaag tggaaatttt
     7801 ataccttatg caatatttgc atcagttaag atggtctcag cttttggagt tacaatagct
     7861 actgttttag gcagagattt attttcagaa tcagaaaagg aaataggaaa gcaaacttgg
     7921 atattaggat tggctggaat aactgaaggt gcaataccat ttatgataga ggacccaatc
     7981 catgttatac catcattagt agctggttca gccataactg ggtctatagt agcattcttc
     8041 aatataggtc ttcaagtgcc tggagcgggg atattctcat tggcgctttt agagggaagt
     8101 atgtcaggat ttattatagc atcaatatgg ttctttgcgg cagtacttgg agctataata
     8161 tcagcaatat tattaataat aactaggaaa caaaaaaatc aaagaaagat acattaaaag
     8221 atacgttatt agatatttaa atcaaaggtt taattatagt gataaaataa atattaaaaa
     8281 attgtgtaaa ctcagtagta tttttattgg atttttaatt agacagaaaa tattatcaga
     8341 taagttttgg aggataagca atgaaaaaaa atgtacacat agttcctcat atgcattggg
     8401 atagagaatg gtacttctca actgaggaat ctagaatatt acttgtaaat aatatggaag
     8461 aaataatgga tatgttagaa aataatcata actatccata ttatgtgatg gatggtcaaa
     8521 ctgctatatt agaagattat ttagcagtta agcctgaatg taaggagaga ataaaaaaat
     8581 tagttcaaga aggaaggcta ataataggtc cttggtatac tcaaactgat gaaatggtag
     8641 ttggtggaga atccatactt agaaatttac tgtatggaat aaaagattgt gatgaatttg
     8701 gagaatatat gaaaatagga tatttaccag attcatttgg tcaaagtgct caaatgccgc
     8761 aaatactaaa tgggtttgat ataaaatact caatgttttg gagaggatgt tctgaaagaa
     8821 aaggaactaa taaaacagag tttaattgga agagtgatga tgggtcttct gttttggttc
     8881 aaatacttcc attaggttat gcaataggaa aatatctacc aatgaatgaa gatgagctaa
     8941 gaacaagaat ggataaatat ttaccggttc ttgataaggg tgcaactact gaccatataa
     9001 tacttccaaa tggacatgac caaatgccaa ttcaaaagaa tatatttgaa gttatatata
     9061 aattaaaaga atgttatcca gagagaaaat ttttcttaag tagatatgaa aacatattta
     9121 aagagctaga aaaaaataag gatatagata caataaaagg ggaattttta gatgggaaat
     9181 atatgagggt tcatagaagt atattctcaa caagaatgga tataaaatca gcaaatgcta
     9241 gaatagaatc aaagataacc aacatattgg agccactagc ttctattgca tattctctag
     9301 gatttgaata tcatcatgga ttaatagagt taatttggaa agaaataatg aaaaatcatg
     9361 ctcatgacag cattggatgt tgttgttcag ataaagttca ccaggaaata atgaatagat
     9421 ttttcttagc tgaggaaaaa gtagaccaat taataacatt ttacaaaaga agaatagttg
     9481 atgcaatgag ttgtgaaatg gctttagata agttagtagc atttaactta atgccttaca
     9541 ataggaatga aatagtgaac gcccaagtta taactaaaat gaaagcattt gaaatatttg
     9601 ataaagataa taataagttg gattttgaag tggtacataa agaagtaata gatgcaggat
     9661 taatagaccg tcaaattgtt cattatggaa actatgattc atttatggta tatacaataa
     9721 actttaaaga taaaatacct gcaatggggt ataaggcgta tatgataaaa gaagttgaac
     9781 atatgttaga aaaagcttat gaagtatgtg acatggtaga taatgacttt tatactattc
     9841 aagttaatga aaacgggaca ttaaagatat ctgataaaaa attaaataaa acttttgaaa
     9901 atgtattatt gatggaaaat ggaggagatg aaggagatga gtatgatttt tcaccacttc
     9961 caaatgagaa attaatattt aacgataata tagtagcaaa ttccactata aagaaaaata
    10021 gatttaataa tgagataaag ataaattata gattaaaagt accaaaagat atacaagcta
    10081 gaaaaaataa tagagcagat tcatttattg actttgatat agttataaat gttccaaatg
    10141 ataaagctat aataaatgta gtattcaata taaataacca agcaagagat tgtagggtta
    10201 gaacttatat accaacaaat atagcttcaa agttttctgt gtctgataat cagtttggac
    10261 atataaagag aagtgtgtat gatgatgcta tggaagtttg gcaacaggaa gaatggagtg
    10321 aaagaccaga ttctatatat ccaatgctaa cattcgttgg attgtctgac aaagagcatg
    10381 gagtagctgt tcttacaaac agtacaagag agtttgaaat agtagggaaa aaatttgaca
    10441 caatagctat aacattattt agaagtgtag gattcctagg taaagaagaa atggcaagac
    10501 gcccaggtcg tccatcagga ataaagttgc ctactccaga ttctcaaatg ataggagata
    10561 taaaaataga cttagcaata gtcactcatg aaaaatctac actagaagca aatgttgcaa
    10621 atatggctaa agagtattta acaaaaatac aaacttataa taaaatacca tataatgcta
    10681 tgaaattgaa tcaatcagaa atagaagtag attattcata tagtttactt aaggaaacta
    10741 ataaggacct tgtgttaagt gttgtaaaaa aggctgaaaa agaagaggca ttagtagtta
    10801 gattttacaa ttctaaagaa gaaacaaaat cagctaacgt agtatttaac aaaaatatta
    10861 aaacagctaa atggactaac ctaaatgaaa aagaattaga atctatatca atagataata
    10921 actctgtaaa tgtagagtgc ctaatgaacc aagttaaaac tatattggtt aaatagtgct
    10981 aaaaatgatt agatagtgct aaaaaggaat tatcttaaaa tagagataat cctccttatc
    11041 attgttgaaa taataaacat atatttaatt ttatacaaaa agagtgtctc atgaactaaa
    11101 aagttcattt tgagacactc ccttttttat aagataaaag aatattaagg gtataagttg
    11161 tatacaaagt gaaatataga gtataaaata aaagataaat taagcttata aaattatata
    11221 tttaaaataa agagtaaata aaaaggagta gataaatatg aaaatattaa tttctataga
    11281 ttctttgaaa ggaagtttat cttcaataga ggcaggaaat gcaataaaaa aggggatatt
    11341 gaaggttaaa gaagatgcgc aagtaaagat attgccactt gctgatggtg gtgaagggac
    11401 agtagatgct ttagttcaag ggatgaatgg taaaaaagag actatagaag ttactggacc
    11461 aatagctaaa aaagtggatg caacatatgg attattaaaa aatacaagta cagcaataat
    11521 agaaatagct caagcatcag gattaacatt ggttccgact gaacttagaa atcctttata
    11581 cactacaact tatggtgttg gggaaataat aaaggaagca ataaataaag gctatagaaa
    11641 ttttatagtt ggaataggtg gaagtgctac taatgatgct gggataggta tgcttcaagc
    11701 cttagggttt gagttttatg atgaaaataa caagttagta ggattaggtg gaaaagtttt
    11761 aaatgaaata agacatataa aaatagaaaa tagattaaag gaattagatg aatgtaagtt
    11821 taaaatagcc tgtgatgtaa ataatccgct gtttgggaaa aatggagctg catacatata
    11881 tgggtctcaa aaaggtgcaa catcagaaat aattgaggag ttagataatg ggcttagaaa
    11941 tttttccaaa gtagttaaaa attacttaag taaagatgta gctaatgtag aaggagcagg
    12001 agcagcagga ggattaggtt ttgctttttt agcattttta aattcaaaat tagaatctgg
    12061 aataaaaata atattagaag aaattaaatt agaggaagag ttaaaagatg cagattttgt
    12121 cataactggt gaaggtagat tggataatca aactgctatg ggcaaggctc caataggagt
    12181 tgcaaaactg gcaaaaaaat atggggtaaa agtaataggt ttggctggag ccacaacaga
    12241 agatgcagtt aaatgtaatg aagagggtat agatgcttat ttttctatag taaatagagc
    12301 tatgacaata gaagaagcaa tggataaagc aactgctagt gagaatatga cagctacaac
    12361 aacacaaata ttcaatctta taactagtat acaacaatca aaaaatatgt agtattaaag
    12421 caaagtatat taggtaaaaa gctgtcacat attaatttag actttttagt gtgacagctt
    12481 atctattttg tgttaataac ttaagaaaca tatattggaa ataaattaat ggatttagat
    12541 attttgaata agataagcta ttctattcat aaaactactg tagttatctg ccttagtaag
    12601 ttctttaaca ttattccact cagataatat ttcaataaag ctttcaaata tgtaattgaa
    12661 tttttctttt tctttttcat ttataggtat catagctata atttgaactt tttcctcacc
    12721 ccatttaact ggtttatcgt taacaattaa acatactcca gtttttaaag catccatttt
    12781 catgctatga ggaattgcta cattgttatt aaatgcagtt gaagacattt tttctcttat
    12841 aagaacatca tctatataat tgggtttgac atatttattt ttgtacagaa tatttcccat
    12901 aaattttatg taatctttag cactatctaa atatatgttt ttcataaaca aagattcact
    12961 aaagtacatt tctaaatgat tttgtaca
//
LOCUS       DAEPLF010000089        12968 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEPLF010000089 DAEPLF010000000
VERSION     DAEPLF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12968
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.089"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..1293)
                     /locus_tag="KR314_002146"
     CDS             complement(<1..1293)
                     /locus_tag="KR314_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBH0328529.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEDLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSI"
     gene            1722..3098
                     /locus_tag="KR314_002147"
     CDS             1722..3098
                     /locus_tag="KR314_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBH0328530.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            3409..4080
                     /locus_tag="KR314_002148"
     CDS             3409..4080
                     /locus_tag="KR314_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBH0328531.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            4271..5068
                     /locus_tag="KR314_002149"
     CDS             4271..5068
                     /locus_tag="KR314_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBH0328532.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
     misc_binding    5412..5646
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5696..6715
                     /gene="pheS"
                     /locus_tag="KR314_002150"
     CDS             5696..6715
                     /gene="pheS"
                     /locus_tag="KR314_002150"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBH0328533.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            6732..9125
                     /locus_tag="KR314_002151"
     CDS             6732..9125
                     /locus_tag="KR314_002151"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBH0328534.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            9150..9740
                     /gene="zapA"
                     /locus_tag="KR314_002152"
     CDS             9150..9740
                     /gene="zapA"
                     /locus_tag="KR314_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBH0328535.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDVYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            10082..10765
                     /locus_tag="KR314_002153"
     CDS             10082..10765
                     /locus_tag="KR314_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBH0328536.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            11257..>12968
                     /locus_tag="KR314_002154"
     CDS             11257..>12968
                     /locus_tag="KR314_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBH0328537.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIETGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MNRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMA"
BASE COUNT         5075 a         1376 c         2351 g         4166 t
ORIGIN      
        1 aatcgataag tgttcttttt ttatagcttc agattgttta ccaaaaaaat catcaaaata
       61 tttataaaat agaatttcta aagatgttga atgaacttca catacatcta tacttggttg
      121 catcttatta tttaagttaa tactgttgta gtaattataa aaatgaccaa actcatgagc
      181 aaaactagta acacctaaaa aagaattgtc ccatgtatta aacaagaaag gtgctttata
      241 tttatttata taagtagtat atcctccagg cgatttgttt tttccagaag aataatcgta
      301 caagtcatat tttttcatat agtcgaagct ctcttttagt tttggagata tatcttcaag
      361 tactttatca aacttcctaa atgagcgatt tttatatact ttaatataaa tagaagaatc
      421 agatggttta gatgaaatct ctctatataa tggaactatg taatctttta catcttgtct
      481 aaatttttta gcttcttctt gagaatagtc tttgtttagg ttcatatagg cataatctat
      541 ataattctta aatccttgct tacttgctat ttcagttctt gtctgtataa gttctaagaa
      601 tatgttacca aatataggat tgtatttttt taagtaatct gaataaattt tcacatattc
      661 ttcagggcta agatttggtc ttgacaaggc ttcttcaaag tctacctcta taccatctat
      721 tgatactgta gatttagcta ataagtcttc atattttaca actaggtctt gctctttttt
      781 aagtaaatct tcaacttctt tagaattaag ttttccaatg ttctttagat aattaaatgt
      841 atcctttcca agttcttttt ctaattcttt agagaattta gaagttacaa atctatcttg
      901 aaagtctttg tatgacatgt caacatctac tgatttactc ataagatact tatattcctt
      961 agagtaaaat tcgtctgatg aatctatgtt atttctaatt tttgctatag taagtgtact
     1021 gtaaaaatcc tgataaattt tatcaacttc attaaataaa tttaattgtt ccttagctgt
     1081 tttagctgtt aaaagtttgt cgttacactt gttaatgttt tcgcaaattg actgtatatt
     1141 aggacgctta tattccattt tttcaaaagg aatagttcct ctaacagaat ctttttttgt
     1201 atcattagta taatttctta tgtatgatgc aaatataata aaaactgcaa ttaaaattag
     1261 aaaaacaaat attttcttct tggatttaac cataaaaatc ctccaatcat ggatatagtt
     1321 attaattatt ttactatatt aaatataaaa aatgcatgtt aagaaaattt aacataaaga
     1381 tatagataag attataaata atataaaaaa tttaacttca aaaaagtata tgaaatttat
     1441 actggtaaaa tgataaataa attttaagta caaaatagta aaagctatga tagttttaga
     1501 cccattatat gtaaaaaata tagatgtttt tggtgcaatt taaattaaaa gatatatata
     1561 aaaatttaaa atattccaaa cagaaattat aaaaattact atcattgtct ttatgcaata
     1621 tttatactat aatagaataa taatttagag gaaagctcta aaaataaaca aattaagggt
     1681 cttaacagta gtgattttat gttaaggagg tataaagaaa gttgaaaaca atagttagaa
     1741 gacttattct ctacgcagat ggaatgcgtc ctactcaaat aatggtatct gggtttgctg
     1801 cgattattgt aatcggagca ttgttgttaa cactcccgat agcgtcgcag agtggagaga
     1861 gtataggctt attaaatgct ctatttacgg ctacttcagc ggtttgtgtt actggtctag
     1921 ttatggtaga tacagcaact tattggagtt tatttggaca gatagttata ataacattga
     1981 tacaaattgg tggtcttgga tttatgaccg tagccacaat gttttcactt atggcacgaa
     2041 aaaaaataca actaagagaa agattattaa tacaagagtc attaaatcaa gctgatttat
     2101 cgggacttgt aagacttaca agatttgtat taataataac cataacaata gagggaatag
     2161 gtgcactggt tttatcaact gtctttattc cacaatttgg tttgtctaaa gggatatggt
     2221 atagtgtatt tcacgcaata tcagcgtttt gtaatgccgg atttgacttg atgggcagtg
     2281 taagtggccc atttacatcg ttaaattctt atgtaaacaa ctttacagta tcaatgactg
     2341 tttgcgcact tatagtgctt ggagggctag gattccctgt tgtacttgat attgtaagaa
     2401 aaagaagatt ttcaaaatta aatgtgcatt ctaaggttgt attgttttca actgctacac
     2461 ttatttttgt gggtgcatta tttatttttc ttatagaatt taatcaaaaa gctactatgg
     2521 cggacttgcc tttaaaggga aaagtactat cagcaatatt tcaatcagta acagcaagaa
     2581 ctgctggttt taatacactt gatttagcta ctttacgaga aagtagtgta tttgtaatga
     2641 taattttgat gtttattgga gcatcacctg catcaactgg tgggggtata aaaacaacaa
     2701 cactagctgt tttaatcatt acagttagaa gttttctatc aggaaaatca gatatagaag
     2761 cttttgaaag aagattagct ccatcaacta taaaaaaatc tcttggtata tttgtaatca
     2821 gtatatcagc agttattttt ggaacattga ttatttcaat aactcagcct aattttacat
     2881 tagttcaatc agcttttgag gttacatcag cacttgctac agttggttca agtttagctg
     2941 gaactccaaa tttaaatgct ttaggtaaga ttataataat aatctttatg tttatgggta
     3001 gagttggttc tcttacttta tttatggcta tactttctgg tggtagaaga aagagtcaac
     3061 caatcagata tgcagaaggt aaaattatgg ttggttaagt taccaaggag taatataaat
     3121 ttataatgga tttagagtag cactgtaaat ctgttgataa aataaatgag cctagatgta
     3181 attatatgaa gaaataaaat caactaataa acaaaattaa ttaatgaaat aaaaaaacag
     3241 ggaaataaaa tacaaataaa aaggtaaatc aaaattaaat taatctgtgt atataaaatt
     3301 atatattgtg tataagtttt gtatgtgtaa agtatataag ttttgtatgt gtaaacaatt
     3361 aatttaagag atattatata aattgtgtat tagggaggaa gtttcgagat gaagcaatat
     3421 atagtcattg ggtgtgggag atttggaagt tcagttgcgt ctactatgca tcttttagga
     3481 catcaagtaa tggcaataga caaaaatgaa gattcagttc aaagtatatc tgacaaggta
     3541 acccattcac ttatagtgga tgttactgat gagcaagcgt taaggtcatt aggtttaggt
     3601 aactttgatg tagcagtagt tgcaataggt tctgatataa gggcatctat aatggcgact
     3661 cttatagcca aagaaatggg tgtagagttg ataatatgta aggcaaagga tgaattacaa
     3721 gctaaagtgc tttataaaat tggcgcagat agagttgtat ttccagaaag agatatggga
     3781 gtaagagttg cacacaattt agtttcggat aatatattag accatattga acttgaccca
     3841 gagtattcaa ttgttgaaat cgtaactcca aatagttggg ttggcaagac acttatagag
     3901 cttgaattaa gagctagata tgagataact gtacttgcta taaaaacagg taaaaatata
     3961 aatgttacac cttctccaga tgaggaactt acagccggaa gtatcctagt tataatcggt
     4021 caaaatacta gtataacagc gataacatct ggaaataagg ggataattag aagaagataa
     4081 tttactattt aatatatatt taattgcaat gaaagtaaag agtatcatat aattatgaat
     4141 agttatatga tactattttt atttaatcga aggtagtatt tatttgtaag attagataaa
     4201 gagaagttaa attaaaagta aggaggctgt gctaatataa aaatttataa gttattagca
     4261 tttagataaa atgattacaa atataaacag taaggataat gaaaagttaa agtatacaag
     4321 agcactatta aaatcaaaga ataggaataa agagtcaaag ttcataatag aaggatacag
     4381 aatagtaatg cttgcacttg aatgtatggc aaaccttgat tatgtattta tcaatgaaga
     4441 atttgaaaat aagaaagaac atgtaaaact attagaagat ttggataaaa aaaacacaaa
     4501 gatatacaag actactaata aaaactttaa agaattagtg gatacagaaa atactcaagg
     4561 aataataggt gtagtttcat ttaagaaaaa aaaattaagt gaaagtataa ataaaaaaga
     4621 taaatttgta ttgattttag atagaataca agacccagga aatatgggga ctataataag
     4681 gactgctgat tctgctggag tagatgccat aatagcacta aagggatgtg tcgatatata
     4741 caacccaaaa gtaattaggt ctactatggg ttctattttt gatatgaata taattgatgc
     4801 ttcacaagat gaaactgtgg acatgcttaa atcattggat tttaatatag tttcaagtta
     4861 cttaaataca gaaaattttt atgacaaaat agattatggt tcaaaagtag cattggtgat
     4921 aggaaacgaa gcaaatggaa taaatgaaga acttgtatca aagtctgata ttttggttaa
     4981 gatacctata tatggtaaag ccgagtcgtt aaatgctgcg ataagctctg ctatactgat
     5041 gtatgaaata aaaaaatact taatttaatg tattgtaata aatataatgt catgttataa
     5101 tcttgaatta aatagtagag tatcaaaaat agtcaatata tattaaaaaa ataattaaat
     5161 taattatata ttagatgtat atcatataaa aaatgattga gttgattatg tattagatat
     5221 atattatata aaaaataatt gagttaatta tgcattagat gtatattata taaaaaataa
     5281 ttgagttaat tatgcattag atatatatta tataaaaaat aattaaatta attatgtatt
     5341 ggatatatat taaaaatagt cacataattt gaatggaaat gatataatac taaaataaac
     5401 aatataatat tgtaaatgca atgaaagagg aaagtatttt gattaaactt agtaaagaga
     5461 taaacaccta ggctgggagt gttttctaag aggtcatgag aagttccctc tggagtaaca
     5521 gagctgaaat tttacagtag gctttgacgt caaaaacgcg ttaagtttgt tagaggtggt
     5581 ttgatgattt ttaaattgtt aaactactag ggtggtaccg cgaaactata gcctttcgtc
     5641 cctagacagg gattgagggg ctttttttat acaaaaaaac gaaagggtga tatgtgtgca
     5701 agaaaaatta cttgctttac gtgaagcagc tttggctgaa ataaaagaag cacaaagcat
     5761 agaaagtgta gaaagtttaa gagttaagta cttaggaaaa aaaggtgaga taactgccat
     5821 acttaaagaa atgggtaaat tatctgctga agaaagacca gtagttggta aggttgccaa
     5881 tgaggtaaga gaaaacattg aacttagcat aaattctaaa aaagaagaaa taaatgctat
     5941 tgaaaaagaa agaaaattaa aagaggaagt gatagatgtt actcaaccag gaaaagtttt
     6001 aaaggttggg aagaagcatc caataactca aattatagat gaagtaacag atatatttat
     6061 cggaatggga ttctctatag cagaagggcc agaagttgag actgttgaaa acaactttga
     6121 cgcattaaac gctcctaaag accatccatc aagagatatg agcgatacat tctatatcaa
     6181 tgatggggta ttacttagaa ctcaaacatc tccagttcaa gtaagaacta tgagaagtca
     6241 agagttacca ataaaagtaa ttgcaccagg tagatgtttt aggtcagact cgccagatgc
     6301 tacacactca ccaatgttcc atcagataga agggcttgtt gttggaaaag atgttactat
     6361 ggcagaattt aaaggaacta tggatatctt cgttgaaaaa ttgtttggtt ctgatatcaa
     6421 aactaagttt agacctcaca acttcccatt tacagaacca agtgcagagg ttgatgttac
     6481 ttgtttcaaa tgtggtggta aaggttgccc aatgtgtaaa tatgaaggtt ggatagaaat
     6541 attaggttca ggtatggttc atccaaatgt gcttagaaat tgtggaatag acccagaagt
     6601 ttacagtgga tttgcatttg gagttggggt tgaaagactt gcaatgctta aatacgaaat
     6661 agatgatatt agattattat tcgaaaatga tatgagattc ttaaatcaat tttaattagg
     6721 agggtatgta gatgttagta tctttaaaat ggcttagaga ctatgttgat atagacatgg
     6781 atgtaaaaga gttcgctgat aaaatgacaa tgacaggaac taaagttgaa acaatagatt
     6841 attatggtga agaaatagaa aatatattgg ttggaaagat tttagaaata aaacaacatc
     6901 caaatgctga taagttggtt gtaactaaag tagatattgg agataaagtt gttcaaatag
     6961 ttacaggagc tacaaatata tcagaaggag attatattcc agtagctgta aatggttcta
     7021 agttacctgg aggagttgaa atcaaacaga ctgatttcag aggtgaatta tcagatggta
     7081 tgatgtgttc agcagctgaa ctaggtatag atgaacatta cattgaggag tataaaagag
     7141 gtggtatata tattttagac cacgaagatt cttatgaatt aggaaaagat ataaaagatg
     7201 ttttaggatt aaaagatgct ttaatagatt ttgaattaac ttcaaacaga cctgattgta
     7261 aatgcatgat gggtatagct agagaagcag ctgcaactat aggaacaaaa gtaaaatatc
     7321 ctgaaatcga agtaaaagaa agtgacgaag agatagattt caaagttgag atagataatc
     7381 cagatttatg tagaagatat gttgctagaa tggttacaga tgtaaaaata gaaccttctc
     7441 catattggat gcaaagaaga cttacagaag caggagtaag acctataagt aacatagtcg
     7501 atataacaaa cttcgtaatg ttagagcttg gtcaaccact tcatgctttt gatataaatc
     7561 aagtagagac tggaagaata gtagtaagaa atgctaaaga tggagagaaa cttgtaacat
     7621 tagatgatgt tgagagaaca ttagataaag atatgctagt tataacaaat ggagaaaaat
     7681 cacttggttt agctggtgta atgggtggtg ctaactcaga aataacttct aatacgaaga
     7741 ctgtactttt tgaaagtgcc aatttcaaac cagaaaacat aagaatgaca gctaaaaaag
     7801 ttggtattag gtcagaagca tcttcaagaa atgaaaaaga cttagaccct aatcttgcag
     7861 agatagcagc aaatagagct gcacaacttg ttgaaatgtt aggagcagga aaagttttaa
     7921 aaggtgttgt agatgtatat ccaaataaac cagaacctaa aaaattggta gtaaatcctc
     7981 aaagaattaa ccacctatta ggtgtagatg taccaatgga gcagtttgta ggaattttag
     8041 aatcattaga gtttaaatgt aatttggtag ctaatgataa attagaaata gatgtaccaa
     8101 gctttagaac agatatggaa caagaagctg atgtatggga agaaatagct agaatttatg
     8161 gatttgagaa tataccttct gtacaattag aaggaaatac gacagcagga attaaaactt
     8221 caaaacaaaa attcatggat gctttaaaag ataattcaac tgctgtagga ttaaatgaaa
     8281 tattaacata ttcatttgta agccctaagg gagtcgataa gataagagta ccagaaggta
     8341 atgctaagag aaactttgtt aaattactta acccattagg agaagaaact tctgtaatga
     8401 gaactacttt aataccaaat atgttagatg ttttatcaac taatgtatct cataaaatag
     8461 aagaagtatc tgcatttgag tgtggacata tattcatccc acaagattca gaattaccta
     8521 aagaagaaaa tagaatgtgt gtaggtatgt atggtaaaga tgttgacttc tttgcactaa
     8581 aaggaactat agaaactata cttgtaaatg tcggatttaa aggttatgaa attgaacctc
     8641 aagataataa tactaccttc caccctggta gatgtgctaa aatagtgtat aataataaat
     8701 atgtaggtac attaggtgaa ttgcatccag atgttataga aaactacaat ctaggtcaaa
     8761 gagtttatgt cgctgaaata gatattgact ttgtatttga taattctgat agaacaaaga
     8821 attatgtgcc attaccaaaa tatccatcta catcaagaga tatagctctt attgttaaag
     8881 atgatgtatt tgtaaaacaa atagaagata taataaagga aaatggtcaa ggattagtag
     8941 aaagctacaa gttatttgat gtttacaaag gttctcaaat agaagctgga tacaagtcaa
     9001 ttgcgtattc aataacttat agaagtaaag ataaaactct aacagatgaa gatgtagcta
     9061 aggtgcatga taagatatta agtgaattaa gcgaaaaatt aaatgctaac ttaagatcaa
     9121 attaacaact aatgtcgggg gaggttagga tgaacaaagt aatggttaaa atccatggcg
     9181 ctgaatatcc aatggttgga gacaaatcag agaagtttat gattagtatt gctgattttg
     9241 tagacaaaga aatggataaa ataacacgtc aaaacccgaa attgagctta tcagttgctg
     9301 cgatactaac tgcattaaat atatcagacc ttttatttga atgttcagat gagaatgaga
     9361 agttgattaa agctaatgaa gaattgagta agaaggttgg agcatctaat gaagagcttc
     9421 aattagaaat caagtcttta aagcttacga tagcagaaaa agaagcagaa aatcgtgaag
     9481 ctgagactaa gatgaaagag ttaattgaga ttatagaaaa taaaaagcaa gaaatattcg
     9541 aacttagtaa tacgacagaa ggttctagag cagagttaga tgtttataaa aacaagattg
     9601 aggagttaag tactcaactt gaagaagcaa atgaaagagc tactatagct gaaaacttag
     9661 cttctgagtt tcaaaacaaa gcttatgatt tacagttaaa atgtacaggg ttaaataatg
     9721 atgcaaaaaa tgtggaataa actaaaagat ttgctgtctc ttgttttttg agacagcttc
     9781 ttttaatgta taaatataaa tgctatataa atatagctaa tgtttaaatg taaataaata
     9841 taaatgctat ataaatatag ataatgttta aatgtaaata aatataaatg ctatatagat
     9901 atagataatg tttaaatata aataaatatg aatgatatat aaatacaaat aatgtataaa
     9961 tataaatgat gtataaatat agataatatt taaatataaa taattttttt atatcaatga
    10021 aaaatagaat ctaaaatgat tttgattttg tatctatcat tagaaagcga gaggtttata
    10081 tatgacaagc aagagtcaga tgtctgaatc attcttttta tgttctttgt tggcaataac
    10141 aggaggattt ttggatattt atacttatgt ggtaagaggt aaagtctttg ctaatgcaca
    10201 aactggaaat atagttttat ttgggcttaa tatagcagaa ggtaatgtaa aggaatcatt
    10261 ttattatttg attcctattt tggcatttat gttaggtgta tttatagccg agaagataag
    10321 aaagtatttt acaaataata agacaaagat tcattggcgt caggttatca ttataattga
    10381 aatgattaca atacttattg tttcatttat tcctagagga agatttaata tgtttgtaaa
    10441 tgttgctatt tcttttattt gttcaatgca agtagagagt tttagaaaag taaatgggaa
    10501 tagttttgca acgacaatgt gtactggaaa tttaagaagt gctacagaaa ctttatttca
    10561 ttatatacat actaagaata tttttatgat aaaaaagagt ttacagtatt atgctattat
    10621 tatattcttt atatctggag ctattttagg aacttttttt acaaaaattt tcgtggaaaa
    10681 atcaatacta atatgttttt ttattttggc agtggtcttt ggaattatgt ttatcaataa
    10741 agatgattca tttaatgaaa attaactttc tgtaaattta caaaaaaatg aagctgttat
    10801 ttgatatact ttatatatag caaatattgt atagattaat taaagaattt aaaatatagt
    10861 aagcaatata taagttaata gaagtatttt aagtatagta agcagtgtat aaattaatta
    10921 aaatgtctta agtatagtag gcaatatata gattaataga agtatttcaa gtatagtaaa
    10981 cagtgtacaa attaattaaa atgttttaaa tttagtaagc agtatataaa ttaattgaag
    11041 aatttaaaat acagtaatca atatataagt taatagaaat atactaaata tagtaaataa
    11101 tgtatatgtt aactcaaatg gaccaaatat aaataactaa tatataaatt aattgaagaa
    11161 tttaaaatat agtaagaaat atataaattt aatacaaata tttaagttat agatataatt
    11221 atattaacct aacaagtata aatagaggag aaaaatatga aaaaaataga attacttgca
    11281 ccagtaggct cttttgattc attaaaagca gcagttcaaa acggagcaaa tgctgtgtat
    11341 ttaggtggta aagactttag tgctagagca tctgcaaata actttgatag aaatgagctg
    11401 attgaggctg ttaaatactc tcatataagg ggagtgcaag tctttgttac tgtaaataca
    11461 ttgataaaac agtatgaaat agaagatttt gtagagtatg taaaattttt atacgatata
    11521 gatattgatg ctttaatagt acaggatata ggaatggcaa gacttataaa aaaactatta
    11581 cctgactttg aactacatgc aagcactcaa atggtagcac attccttaga agatgtaaaa
    11641 taccttcaga gtgtagggtt tgatagagtt gttttagcaa gagaattaaa tgttgatgag
    11701 ataaaatgga tttgtgaaaa tacaactgta gatatagaag tttttgtaca tggagcttta
    11761 tgtgtatgtt actcgggtca atgccttatg agtagtatga taggcaatag gtctggaaat
    11821 agaggtaggt gtgcacagcc atgtagacaa aagtatgagt tgatagatat aaatacagga
    11881 gaagtagtaa aaagtaatgg agattatttg ttgagtccta gagatttaag tactatagaa
    11941 gatttggata aaataattga aacaggtgta ttatctttaa aaattgaagg gagaatgaaa
    12001 aaaccagagt atgtagctac tgtggtttca ggatatagag aagcgattaa tgaatttgta
    12061 gataagaaaa aaataagtat ttctgaggaa ataataaata atttgtatac catatttaat
    12121 agaaagttta caaaaggata tatattaggt gaagttggta aggatataat gaactctaat
    12181 agacctaata atcaagggct atatattgga aaagttttag attataatag aaagaacaaa
    12241 agacttagag taatgctaga aaatacgctt aagaaaggtg atggaattaa cttaggtgga
    12301 ggtactattg gaagaataat aaaggatgga gagataacta caatagcaca taaaggggat
    12361 ttgatagaat tagattttgt tggagaagca aagagaaatc agcttatata taagacttct
    12421 gacagcgaat taatgaatag agtacaagct acatttcagc aagataaaga gttcataaaa
    12481 actaatatac gtgcaaaagt gagtattaaa ctaggtaaaa agccagagct ttttataagt
    12541 gatttttgtg gaaataaatc ggtggtagaa ggagacaaaa tagttgaaaa agctataaag
    12601 gtggctttaa gtaaagaaaa aatagaaaca caaattcaaa agcttggaaa tacaccatat
    12661 aagttaaaag agatagaaat tgacctggat gaaggtgtaa gtatgcctat aagtgtgtta
    12721 aatcaaatga gaagggattg tattgagctt ttagatgaag aaagaataaa gataaaagat
    12781 agaaaattta agaagaatag attaaaatac caaccaaata gagtaaataa acagaaaaaa
    12841 cttgatacag ttcctaagat tagagttaaa gttaagaact tggagcaatt agaagcagta
    12901 ttaaattata atgtagattt aatctattat gaagatataa atacattatc aaaagcttta
    12961 caaatggc
//
LOCUS       DAEPLF010000090        12748 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEPLF010000090 DAEPLF010000000
VERSION     DAEPLF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.090"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            46..1674
                     /gene="aspD"
                     /locus_tag="KR314_002155"
     CDS             46..1674
                     /gene="aspD"
                     /locus_tag="KR314_002155"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBH0328538.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDEI
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1795..2790
                     /locus_tag="KR314_002156"
     CDS             1795..2790
                     /locus_tag="KR314_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBH0328539.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGIGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(2948..3616)
                     /locus_tag="KR314_002157"
     CDS             complement(2948..3616)
                     /locus_tag="KR314_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBH0328540.1"
                     /translation="METVSNNQSSVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(3715..4027)
                     /locus_tag="KR314_002158"
                     /pseudo
     CDS             complement(3715..4027)
                     /locus_tag="KR314_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431045.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4454..6874)
                     /locus_tag="KR314_002159"
     CDS             complement(4454..6874)
                     /locus_tag="KR314_002159"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBH0328541.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(7228..7578)
                     /gene="rsfS"
                     /locus_tag="KR314_002160"
     CDS             complement(7228..7578)
                     /gene="rsfS"
                     /locus_tag="KR314_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBH0328542.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(7695..8267)
                     /gene="yqeK"
                     /locus_tag="KR314_002161"
     CDS             complement(7695..8267)
                     /gene="yqeK"
                     /locus_tag="KR314_002161"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBH0328543.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSRNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(8268..8957)
                     /locus_tag="KR314_002162"
     CDS             complement(8268..8957)
                     /locus_tag="KR314_002162"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBH0328544.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            9233..9871
                     /gene="recX"
                     /locus_tag="KR314_002163"
     CDS             9233..9871
                     /gene="recX"
                     /locus_tag="KR314_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBH0328545.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            10024..12027
                     /locus_tag="KR314_002164"
     CDS             10024..12027
                     /locus_tag="KR314_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBH0328546.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(12330..12641)
                     /locus_tag="KR314_002165"
     CDS             complement(12330..12641)
                     /locus_tag="KR314_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBH0328547.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
BASE COUNT         4424 a         1997 c         1743 g         4584 t
ORIGIN      
        1 tttattataa aaatgtgaaa cttattacat aaggaggaat ttaacatgaa aaatcatact
       61 tataaagaaa tcaaaaacat atatggaaaa ataagccctt ttgaatttaa ggacaaatta
      121 atagatattg caaaatacac tgcaaaagaa aataacagag aattacttga tgcaggtcgt
      181 ggaaatccaa actggacttg ttcaactgct agagaagcct tttttacttt tggtcatttc
      241 gctattactg aaactcgttc taattgggat ttaggtcatt tggctggaat gcctcaaaaa
      301 aagggtatta aagaaagatt ttttaaattt ataaatgaaa atatagatat gcctggagca
      361 tatttagcta gagatattat aaactttggt ataaatgaac ttggatttga tggagatgaa
      421 tttgttcatg aactagctga tggaattatt ggtgataact atccacttcc agatagaatg
      481 ttacctcata tggaaaaaat agtacacgat tacctagttc aagaaatgaa atatgatata
      541 tcaggtaaat atggtgatgt agaaattttt gctgttgaag gtgctacagc tgctatgtgc
      601 tatatatttg attcattaat ggcaaatgaa cttctaaaaa aaggagatac aatagcctta
      661 atgactccaa tatttacacc ttatcttgaa atacctaacc ttcctcgtta cgactttaaa
      721 gtggtaaata taaatgctaa tgaagttgat gaaaaaggtg ctcatacatg gcaatataca
      781 aaaaaagaat tagagaaact tcgtgacaaa tctattaaag ctttattcgt tgttaatcct
      841 aataatcctg cttctatagc tatggatgaa atatcttgta ataatctaat agatgtaatt
      901 gaaaattata ataaagattt aatgataata tcagatgatg tatatggaac ttttgtagaa
      961 gatttttcat ccttaatgtc aaaactacct tacaacactg ttggagtata ttcttattca
     1021 aaatattttg gagttacagg atggagactt ggtacatttg cccttcataa gaaaaatgtt
     1081 tttgataaga aaataaatga tttaactgga gaattaaaaa aatcagtaga taagcgttac
     1141 agtgatatga gtcttaaccc ttctagtctt tcatttatgg aaagagttgt tgctgatagc
     1201 agacttgttg ctctcaacca tactgctggt ctttctactc cacagcaagt acaaatggcg
     1261 tttttctctg cttttgcatt gattgacaaa gtagatgcct acaaaaaact caatatgagt
     1321 atctgccata gacgtcaaaa actattattt gaagctttag agcttccaat taatgaaaac
     1381 aaaaacaatg ctgcatatta cactcaattt gatattgaag aatgggctaa attaaactac
     1441 ggagaagact tatttaaatt tataagtaaa actgcatctc cagtagatgt tctttataaa
     1501 ttagctaatg actattcagt agttttactt agtggaaatg gattctatgg ccctgaatgg
     1561 tcaattagaa tatctctagc aaatctatat gatgaagctt atactaagat aggaaaagcc
     1621 ataagagaga tattaaatgg ttatatcgtt gaatggcaaa aaattaagaa ataattatat
     1681 tacactgaaa tatctaaatt atatgaataa aagagtttat gtagacctta actacttttt
     1741 ttgatataat cctatcggaa ttataaattt tatttatgaa aggcagagaa tcgtatgagt
     1801 attaagaaaa aagttgccat agtttttact ggtggaacta tatctatgac agtagatgat
     1861 aaagtcggag cagctatacc aactttatca ggtgaacaaa tactatcaat ggttaccaac
     1921 attgataaag ttgcagatgt tgaagttttt aattttgatg aaattccagg acctcacata
     1981 acaccttata gaatgatgga attgaaaaat tatgtaaatg atttattagc aagaaaagat
     2041 attacaggtg ttgtaataac acatggtaca gatagtttag aggaaacagc ttattttcta
     2101 gaccttacaa ttgacaacat caaacctgta atagtcacag gtgctatgag aagtagttct
     2161 gaacttggat atgatggttc aagtaattta tctgcatcag tttgtactgc agtatccaaa
     2221 gatgctatgg gaaaaggagt ccttgtagtt ttaaacaatg aagtattatt agcttctgaa
     2281 gctactaaaa caaatactct ttctctaaat acatttaaat ctctaactag tggtccatta
     2341 ggaataattg actgcaacga acttgtcctt acaagagata ttgtaaatag aacaattata
     2401 gatactgata aggtagaatc taaagttgcc ttatttaaag catatgtagg agaaaatgct
     2461 gactttataa gatttgctgt tgatagtggt tataaaggta tagttattga ggcaatgggt
     2521 ataggtaata ttcctcctca aatgctttct ggagttgaat atgctagaga aaaaggactt
     2581 cctgtagtta tagtttctag atgtcattca ggtagagtat tcgatagtta tggttacttt
     2641 ggttctggta gagatttaaa aaatcttggt tgtatatttg gtggggactt gccaggtcaa
     2701 aaagctagaa taaaacttat cttagcactt ggaaaaactg ataaccttga tgaaatcaaa
     2761 gatttctttg aaaaaggaat ctattgctaa ttctaataac ttctatttaa ttatttagtt
     2821 cagtttacaa gaaaatacac acaaaccata aaatgagatg ttgacaacat aacagacagt
     2881 atgctcctgt aaaaggtaac atactatctg ttatattgtc tggattccca aattagctat
     2941 ttggaactta actaaaaaat cctaatataa gggcaactat tataatggca atcggaatac
     3001 atactgtgta atcctgttcc atagagtcat catctgaatg tagttttaca attcgcaatt
     3061 tagcaatcag tttgaagcag aaatagccta ttattgttcc aattacattc gtgagtaagt
     3121 cgtctatatc ggttgctctg tgtaacgtaa aaagctgaat agtttcgata aagagagata
     3181 atccaaaacc aatcaagaat gtatttctca accgttcaaa ggttttgctg ataagcggac
     3241 ataaaaaacc taatggaaca aaaagtaaaa tattcaaaat aaaactcaaa ctaaatccat
     3301 cactaaaagg aataagattt atgtttggat taaagaacgt ttcccctaac tgtgacagtc
     3361 ttatatattc actgaatgta gggataccaa caatatgcga tagtgttaaa cataagtagt
     3421 aatacattgc cagtgataaa atagatgttt ttacctttgg aatatcacta tttttcttga
     3481 atattacaac taagaaaata ccaatcaaga taattcccaa caagaaaata ggattaatta
     3541 acaataaatc tttgatagct tcaaacagac tgagttgaat ttctacactt gattgattat
     3601 ttgatacagt ctccatttgt acacaccccc tcataacatt tcaattattt ctaaaaaaca
     3661 aaatagaaga taaatcttaa aatcatccta tcaaaatttt taaatttgcc tttatcagtt
     3721 tataccacca gttaattcct gtcttacttc tgtatggtat tcttgggatt ctttcatcaa
     3781 attagacaaa aaaatggtta ccatatcaat aaactggtta cgctccatat ctcctgaagc
     3841 aaacatcccc tgagaataaa tccgtatcag gaatctccac atctactgca cctatcacaa
     3901 gtcatttgat aacatcctca aacaatccca acttggatat atcagcaaca cccattagaa
     3961 tactactgct gaccatcctt gttttcctgg tgataatttt aatcaactgg gaagttgaaa
     4021 cgcctatctt ttttgcaagt tcctttttag aaacatggta gttattatat gaatctgaat
     4081 ctcgttgtct gggtgtttaa gataaagcat agatacgcac cttttttgcc tgttttcatt
     4141 atataaaaaa aattttaaaa atacaataat tatatttttt tcgtttaagc ttagagcagt
     4201 tccttgatga acaacacttt agaaactgaa taatttacat accacttaat acattaacac
     4261 gatttaacat taattataat aatatttaaa acaacaatta gagtgtataa gaatgaactt
     4321 tttagttctt taatcatttt tttgtataaa atcaaatcta tttttgtaat ttcaacaatg
     4381 aaaaaaggct tatctctatt ttgagacagc ctcatttatt ttataaattt atatttttgt
     4441 tcatattttt gagttattta acaactatat ttactaattt tttaggtaca gctacaactt
     4501 taactatagt tttaccttca actaatcctt ttattttttc atcttctaaa gctacctttt
     4561 ccatctcatc tttagatata tttgagttaa cagttaattt tcctctaact tttccattaa
     4621 cttgtacaac aacttctatt tcatctttta ctaaagcttt ttcgtcatat ttaggccagt
     4681 ctatatcaaa tacacttcct tctttaccta tcatagtcca tagttcttca cctaaatgag
     4741 gggcaaatgg tgcaattata gttactatag tttgtacacc ttcttttata actgcttcgt
     4801 ttatattatc taactcttta tatttataca tatcatttat taattccata agagctgata
     4861 tagcagtatt aaatccgaat ttttcactta aatcagcagt aactttttta agtgtagagt
     4921 gtatagtata tctcatatcc ttatcttggg aatttaattc tccaaactct acatcctttt
     4981 taaccacatc tgctaactcg tcaaccaatc tataaaccct atttaagaat ctaaagcatc
     5041 catcaacacc ttgctctgac cagtctaaat ctctttctgg tggtgctgca aataatacga
     5101 aaagtcttgc agtatctgct ccatactcat ctattatatc cataggagat actgtattcc
     5161 ctttagactt actcatttta gaaccatcca taagtaccat tccttgagta agtaagtttt
     5221 taaatggctc attgaagtct accattccca tactcttaaa tgcctttaca aaccatcttg
     5281 cataaagcaa atgcattata gcatgttcta ctccacctat atattggtct actggatgcc
     5341 atctattaac taattcctta ctaaatggtt cattttcatt attgctatcc acatatctta
     5401 agaaatacca agaagaatca acgaatgtat ccatagtatc aacttccctt ctagctggtt
     5461 ttccacagtg aggacaagtt gttgacataa attgctttga agtcgtaagt ggagattctc
     5521 cctttccagt aaattcaaca tctgttggta aaagaactgg taaatcttcc tttttaactg
     5581 gaactattcc acattcatca caataaacca caggtatagg acaaccccag tatctttgtc
     5641 ttgaaactaa ccagtctcta agtctataat taattgttct ctttcctcta ccttcttttt
     5701 ctaacttatc gattattcct tcaaatcctt ctgaagcatc taatccatta aattcttctg
     5761 aatttatcat tttgttatct tctgttatta catcaattat gtctaaatta tatttttcag
     5821 caaaatctct atctctttca tcatgtgctg gaactgccat tatagcaccc gttccatact
     5881 caactaatac atagttagct atccatagag gaactttctt gccatttaaa ggatttataa
     5941 catatctacc tataaacatt ccttcttttt ctacatcagc tgctgttctt tctatttctg
     6001 tcatagtatg cattttttga acaaaagctt caacatctgc ttcatattca gtccctgcaa
     6061 ccaattcttt aactaattca tgctctggag caagtaccat ataagtaact ccataagttg
     6121 tgtctggtct agttgtaaat actgtcatag atttatcagt tccatctata tcaaaaacta
     6181 aatctgctcc tgtactttta cctatccaat ttttttgcat tgttttaact ttttctggcc
     6241 atccatctaa tgtatctaag tcattaagta attcttctgc aaattcagta gtcttaaaat
     6301 accattgttc taaatctttc tttaatactg ttgctttaca tctctcacat gctccttgaa
     6361 caacttgctc atttgcaaga acagtctcac aagaaggaca ccagttaaca tatgattttt
     6421 tcttatatgc aagcccatgc tctaaaaact tcaaaaatat ttcttgtgta aatctatagt
     6481 attctggagt tgaagttgca acttctttat cccaatcata actaatacct aataaattta
     6541 attgctcttt catttcttct atattttcca ttgtccattt atgcggatgt attccatgtt
     6601 ttatagctgc attttcagct ggtaatccaa aagaatccca tcccattgga tgaagtacat
     6661 tatatccttc catttttttg aatcttgcga caacatcacc tatagaataa tttctaacat
     6721 gtcccatgtg tatttttcca gatggataag ggaacatttc taatgtgtaa taattaggtt
     6781 tttcagtttg tgcagtatcc attttatact gattattatc tttccaaatt ttttgccatt
     6841 tcgattctac ttctttaaaa ttatatacgc tcatattttg cctcctattt ttaattgtgt
     6901 ataactacct tatttagtat tatttactta taatttccaa ataattcttt gaaaataaaa
     6961 aatcctcgtc actaaactta cgtttaggga cgaggataaa tcttcgcggt accaccctaa
     7021 ttagcctact acatatatag actctcttag atagctttta atccagccat ggagaataga
     7081 ttttaatcta gtaagttcaa agttatataa tattagttct caccttccac taactctctg
     7141 aaaaaatata atcttttact gctctagtct attcaattta atataatttt tgaaattttc
     7201 taattataat aacatatatt atcatgttta tgctaatgtg tcaatatcta tataaggtgc
     7261 atctttccat aatttttcta aattataaaa ccctctattt tcctcattaa atacatgaac
     7321 cattatgtct ccataatcaa ggagtaccca agtttgagtt ccttgtcctt ctttacctct
     7381 tggctctaat cctagttttg ctaattcatc ttccacattg tcagcaatag cttttacctg
     7441 tctctgagaa gatgctgtag taataataaa ataatcacac aaactagaaa cttttcctat
     7501 atttattata actgtatctt gacccagttt atcttctatg gcatcatatg ctatttttgt
     7561 catttgttcc actgtcatat aaaaattcct cactttccta attaaaatac acttagttat
     7621 tgtaacatat taatacgatt ttgtaacata gtcaaattta aatttattaa tatgtttgcc
     7681 tttatatagc ttttttataa taacttttct ttaattagat agtttcttgc cataactgtt
     7741 cttggatgta tctcttcttt tttattgatg acaaacatta aagtattatt aaaagaagtt
     7801 aaaagtgctt catcaagctt ccctccatat gctaattctc ttaatgtatc tacaactgga
     7861 aattttcttc cttcttcaat taaatcagct atgtaaatta ttttttctaa aagagacatg
     7921 tcctctctcc ctgttgtatg atatttgatt gcatttataa tatcttcatc ttctacttca
     7981 aattcatact cgcatatgta cgccccaatt acactatgag acaaggaacg atttccatcc
     8041 tctattgggt ctaaatatat ttcatactta tttacataat cctctatttc cttgtcactt
     8101 aaatactttg cacaatcatg aatcattcct gctaagtaag ctttttcttt gtcacatcca
     8161 taaatctcac ataatttttc agcacatttt gcaacatttc ttgaatggct aagcctcccc
     8221 actggtagca tttgatttag tctttggtta atattttcta agttcattta atcatctcca
     8281 tattgatata gtttattttc atataaataa ttttctacat tttcgggtac taagtaacgt
     8341 attgacttgc cttcatttag ctgttccctt atatatgtgg acgatatatc cagtgatgga
     8401 acataaactg ttataatgtc agcattatac ttcttagtta gtttttctat agtctcttgt
     8461 gaccttaaca aactaattcc tggtctagtt gcggctataa aagtagctaa ctcaaagttt
     8521 ttctttacat ctttccactc ttcaacacta caaattgcat ctgcacctgt tataaaataa
     8581 atgtctgcat ttttgtattt tttctttaag tattttaatg tatctattgt ataagtcctt
     8641 ttctttcttt cgatttctac ttttgaaact aaaaaatctt cgttgctttc agttgctaga
     8701 agtgtcatat tatatctatc atacttatct gtagtgatac ataatttatg tggtggattt
     8761 cctgatggta taaagattat tttgtcaatg tcatacttgt ctctaataaa ttctgctgtt
     8821 gctaaatgag catagtgtat agggtcaaaa gtacctccca atatacctat tttgattttg
     8881 cctttatggc gattaaattt ctctagtttt tcagtatttt tcaactctgc catcttaact
     8941 agattttcac ttctcatatt ttgtctcctt ttaatgttat aatactattg taccattatt
     9001 ttttatttct taaagcttat aatttgtttt tttgggtata taattatatt tatatgctaa
     9061 aaaacacttt aaaaaataat tatttaacat gattctgctt tactttttaa taaaaataac
     9121 tttttactat tttgtcttaa ttttgataaa atataacata caaatataag ttttttgtta
     9181 aattttatta gttgcttttg ttaatataat tacatataaa aaaggaggac ttatgggtat
     9241 aattacaaaa atagaacaac aaaaaagaaa tgatgataga gttaatattt atgtagatga
     9301 taaatttttt attgctatat ttaaagaact tgtttataca tttaatttaa aaaaagggaa
     9361 tgaaataaat gaagaagaat taaaacctat tttagatgat gaaatgtata taaaagctaa
     9421 aaataaagca ttaaatattt tatctcatgc tgaccaatct gaaaaaaaat taaaagaaaa
     9481 actatcatca gaatttgatg aaaatataat tgagagggtc atagattttc ttaaaagtta
     9541 taatctcgta aatgacagtg tattagctca aaaaattgtt aacacaaatg ttaacctaaa
     9601 taaatgtggc aaaaacagaa ttaagcagaa tttatataat aaaggtatca atagaagtac
     9661 aatcaatgaa gcagtatctg aattagacaa ggacactgaa tttgaaaatg ctatgtatct
     9721 agcaaaaaaa aggtatgaaa gagtaaagaa agaagataaa aagaaaatat atcaaaaaat
     9781 ttctcaacat ctttcctata aaggctttga ttatgatatt attaaaagag ttttaaacaa
     9841 acttttaaat tttgatgaat acgatatata agtataaata ctagggaggt aaattatgaa
     9901 attaatcatt ggtggcttaa gctttttatt aggattatat aatatatatc aattatacag
     9961 agaagatagt atgagataaa tattattttt taaatgtaat atttattatg gaggagaaat
    10021 acaatgaaaa gtcaaaaaag atttaaaatt ttagatattg atgtatacct acaaccattt
    10081 gaatctgata taaaaaagcg aatgaaacat tatgaggata tgaaatcaaa aacattgaaa
    10141 aactataaag attttagttc ttttgcaaat gctcatcttt actatggatt tcatcagaca
    10201 aatgatggat tcttctatag ggaatgggct ccaaatgcag atagtttgtc actaattggt
    10261 gactttaata attggaatag aaaatctcat cctctaagaa aaattgggtc tggtaattgg
    10321 gaaatattta ttcctggtca aaatagctta cctcacaaat cagaggtaaa ggtacaagtt
    10381 actgcaaatg gaaaaacatt tgaccgtata ccattatata taaaaagagt aattcaaaaa
    10441 gattttggtg gatttaatgg acaaatatgg caaccaaaaa ctccatttat ctggactgac
    10501 aataattttg acttaaaaaa cataacttct ccactaatct atgaatgtca tataggtatg
    10561 tctactgaaa gcgaatctat tggaacttat aatgaattta ctgaaaaaat acttcctaag
    10621 ataaaaaaag ctggatacaa taccattcag cttatggcta ttatggaaca tccatattat
    10681 gcatcttttg gataccaagt ttcaaacttt tatgcaatct catctaggtt tggtactcct
    10741 gaagacttaa aaaacctcat aaacactgct cattcaatgg ggattgctgt tttattggat
    10801 ttagttcatt ctcatgctgt aaagaatact ttagaaggaa taaatgagtt tgatggaagt
    10861 gaacatcaat ttttccactc aggttcaaag ggaaatcatc ctgcttgggg tacaaaactt
    10921 tttaactatg gtaaacctga agtaatacat tttttgcttt caaatataaa attttggtta
    10981 aatgagtacc attttgatgg ctttaggttt gatggtgtga cttcaatgtt atatcataac
    11041 catggtcttg gagtttcatt tgatagttat gaaaaatatt ttagtatgaa tacagatatt
    11101 gaagctataa catatctcca atttgcaaat gaattaataa aagaaattaa acctaattcg
    11161 ataagtattg cagaagatat gagtggaatg cctggtatgt gtatccctat aaaagacggc
    11221 ggaattggat ttgactatag actcgcaatg ggagtacctg atttttggat aaaaactatc
    11281 tctaacttat ctgatgaaga ctgggatttg ggaaaaatgt ggtatgaact tactactaga
    11341 cgacctggag aaaaaaatat aggatattgt gaatcccatg accaagcatt agttggcgat
    11401 aaaacaatta ttttttggct agctgacaag gaaatgtact ggaatatgga aataaactca
    11461 aataaccatg ttatcaatag agcaatatct cttattaaat taattaaatt aataactttt
    11521 tctctagcag gtgaaggata tctaaatttt atgggaaatg aatttggaca tcctgagtgg
    11581 attgattttc cacgagaagg caacaattgg agctataagt atgccagaag acaatggagc
    11641 ctttcagaag ataacaactt aaagtataaa caattattaa attttgataa atctatgctc
    11701 gaacttacaa aatacagtga tatttttatc caaaatagtc atgaacttat acatatggat
    11761 aatgataaaa agattttggt gtacagtaag ggcaaatatt tatttatatt taatttccac
    11821 cctaatctaa tacaatcagt tgatatttca aagtttcaaa atacaaacta caaaacgata
    11881 ttaaatactg atatggtaga gtttggagga aacacaaaaa aagaaaactt aagagattat
    11941 gatgtatact ttagctcaac tcctccttct tctaaaatgc ctattgcctc tcgtacagca
    12001 atagtacttt tagataatgg catttaaata tgagccaaat atattaggaa actgcaaatg
    12061 tagtccagta taaggcaaaa aaataaaata atgaaagcat ggcaatatac ctgttcaaca
    12121 tgctaaaaag acatattgac aaatcagtat taagcctttt agctcccaaa tatgacatta
    12181 ttataatcca cactctaaca gtttgttggt ttttctagta tataacactc atattaaagt
    12241 aaacactaaa agccaaataa caatttaaat acaaaatgtt atttggctaa tttagtttga
    12301 tactatttaa ctatctttta cattcagttc tataatttcc aacttttaat ttgtggtatt
    12361 atattcatct taacagtttg attctttaaa gcttttctaa atttctcagt tgaagaatct
    12421 ttaaacattt tactagaagc actcttgagg tcttctatag attcccaatg ttgtatcata
    12481 atccactttt gagacttttc atcatataaa agctcagtat caataaaacc agattgaata
    12541 gaatgaaatt ttaattctaa gtcatttaca atttctataa actcttcttt tgataaattt
    12601 tcttccacat taaactcaac tatttctgta ataattttca tatgtaaaac ctccaattta
    12661 ttttcctata tcatatcaaa aaataaattg gaggtattgt aaaaaatcga cagtataatt
    12721 ataaatagtt gtactttata ttttcttt
//
LOCUS       DAEPLF010000091        12335 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEPLF010000091 DAEPLF010000000
VERSION     DAEPLF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.091"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..454
                     /locus_tag="KR314_002166"
     CDS             <1..454
                     /locus_tag="KR314_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0328548.1"
                     /translation="SKVKKKFGSGKFKVELYKEKSKLNREIDDIQEKKAKIFLEMGIL
                     TYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESNVTCECGYVGNVND
                     KYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIMDLE"
     gene            727..1398
                     /locus_tag="KR314_002167"
     CDS             727..1398
                     /locus_tag="KR314_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBH0328549.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            1483..2349
                     /locus_tag="KR314_002168"
     CDS             1483..2349
                     /locus_tag="KR314_002168"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBH0328550.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            2382..3884
                     /locus_tag="KR314_002169"
     CDS             2382..3884
                     /locus_tag="KR314_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBH0328551.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSVVLAGLTGIPVNVLIVV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFIGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            4014..4466
                     /locus_tag="KR314_002170"
     CDS             4014..4466
                     /locus_tag="KR314_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBH0328552.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            4626..5429
                     /locus_tag="KR314_002171"
     CDS             4626..5429
                     /locus_tag="KR314_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328553.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDALYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACNECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            5487..6374
                     /locus_tag="KR314_002172"
     CDS             5487..6374
                     /locus_tag="KR314_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBH0328554.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEVWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNINGSSFQELASTSSLIKKVAKIKNLNEKDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            6393..7241
                     /locus_tag="KR314_002173"
     CDS             6393..7241
                     /locus_tag="KR314_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0328555.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            7304..7993
                     /locus_tag="KR314_002174"
     CDS             7304..7993
                     /locus_tag="KR314_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBH0328556.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKAKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITFDSSKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            8383..9390
                     /gene="asrA"
                     /locus_tag="KR314_002175"
     CDS             8383..9390
                     /gene="asrA"
                     /locus_tag="KR314_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBH0328557.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEIIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            9391..10182
                     /gene="asrB"
                     /locus_tag="KR314_002176"
     CDS             9391..10182
                     /gene="asrB"
                     /locus_tag="KR314_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBH0328558.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            10195..11166
                     /gene="asrC"
                     /locus_tag="KR314_002177"
     CDS             10195..11166
                     /gene="asrC"
                     /locus_tag="KR314_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBH0328559.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            11223..11999
                     /locus_tag="KR314_002178"
     CDS             11223..11999
                     /locus_tag="KR314_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBH0328560.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGLIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
BASE COUNT         4965 a         1141 c         2284 g         3945 t
ORIGIN      
        1 ttctaaggtt aagaaaaagt ttggtagtgg aaaatttaaa gttgaattat ataaagaaaa
       61 atcaaaatta aatagagaga ttgatgatat tcaagaaaag aaagcaaaga tattcttgga
      121 aatgggaata ctgacatatc aaaaaattag agaagattct attaatgata atagttttga
      181 taaattctgt gatgagttac tagaacttga taagttaatt tatgaaaaaa atatggaaat
      241 taatgaaatg gaaatggcag aaagtaatgt aacttgtgag tgtggttatg ttggaaatgt
      301 aaatgataaa tattgtgctg aatgtggtaa aaaatttgaa ttggaaaatg aatgtgatga
      361 ttttataatt tgtggttatt gtgaaagtga gatagataca gaggctgaat tttgtccatg
      421 ctgtggaaga aaaattataa tggatttaga ataatattgg gaaacctatg tatagtatac
      481 atgggttttt ttattgaaat tatttaaatg gacttataac agtagttata taaaatgaaa
      541 ataaacacca aaaaaatgaa ataaaatttc ttttaaatgt aaaaaacaag ctaatatata
      601 aaataaatca gtaaaaatat agatgcgaga aaaaaatatg aaaaaatatt gcaaagaaaa
      661 agttttcatg ttattattaa tacataaatc aagaaaataa atttcagaaa cgaggtaatt
      721 taaaaaatgc tagataaggt aaagggaaga ttaatagtat catgccaagc tttagaaaat
      781 gaacctcttc atagtccttt tataatgggg agaatggcta aggctgctat ggaaggtgga
      841 gctgttggaa ttagagctca aggagttgaa gatattattg aaataaaaaa agttactgga
      901 cttcctgtta taggtattat aaagagaaac tatgaagatt cagatatata tataacgcca
      961 actaaaaaag aagttgatga acttttaaca acagggtgtg aaatgatagc attagatgct
     1021 accaatagag ttagaccaaa taatgaagac ttaaaagaac ttataaagta tataaaagaa
     1081 aacggtgttc tagtaatggc agacatttca aattatgatg aggctatcaa agcacaagag
     1141 tacggtgtag attgcgtttc aactacacta tctggatata ctccatatac taaaacatta
     1201 gaaggtccag attttgtatt aatggaaaga ttagttaaag atttagaaat acctgtaata
     1261 gctgaaggaa aggtaaatac tcctcaagat ttaaaaaagg tctttgagtt gggtgttcat
     1321 tcatcagtag taggttcagc aataacaaga ccacaattaa taacagaaaa atttgttaaa
     1381 gcaattgaaa ttaattaaaa attaatttat aaattaaaaa attattatat ttaactatac
     1441 ttgaaaatta aaattggagg taaagatatg agaacaacag atatgaaggg aatttattca
     1501 gcattattag tttcatttga taaggaaggg aatataaatg aaaaagggtt aagacaaata
     1561 attagacaca atatagatgt ttgtaaagta gatggtcttt atgtaggagg aagtacaggg
     1621 gaaaacttca tgctttcaac tgatgaaaag aaaagaatat ttgaaatagc taaagatgaa
     1681 gtgaaagaag aaataaaatt aatagctcaa gttggttcag ttaatttaaa agaagctgta
     1741 gaacttgcta aatttacaac tgatttagga tatgatgcaa taagtgctgt tacacctttt
     1801 tactataaat ttgattttga agaaataaaa cactattata atacaataat aaattctgta
     1861 gataatagat taataatata ctctatacca tttttaactg gtgttgatat gagtttagac
     1921 cagtttggag aattatttga aaatgaaaaa ataataggtg ttaagtttac agcagcagat
     1981 ttctacttat tagagagaat gagaaaaaca ttcccaaata aattaatatt tgcaggattt
     2041 gatgaaatga tgttaccagc tacagtttta ggtgtagatg gggcaattgg ttctacattc
     2101 aatgtaaacg gtgtcagagc tagacaaata ttcgaattaa ctaagaatga aaaaatatca
     2161 gaagcacttg aagtacaaca tgtgactaat gatttaataa cagatatatt aggaaatggc
     2221 ttatatcaaa caattaaatt actacttgaa gaacaaggtg ttgaggctgg atactgtaga
     2281 caacctatga aagaagcaac tgatgaaatg aaatcaagag caaaagaaat atacagaaag
     2341 tatttttaat aaaatacttt tagtatggga ggattagtca aatggtagga tttacttgga
     2401 tagattttgc aatactagtg gtttacttat tggcagtttt atttgcagga ttgttatttt
     2461 caaaaaagga aatgaaggga aaagagttct tcaaaggtga tggaactatt ccgtggtggg
     2521 ttacatcagt atcaatattt gccacattat taagtccaat atcattttta tcattagcag
     2581 gaaattctta tggtggtact tggattttgt ggtttgcaca attaggtatg tttatagcaa
     2641 tacctttgac aataaaattt ttcttacctc tgtatagtag gttagaaata gatacagcat
     2701 atgaatactt agaaatgaga tacgaaagta aaggtcttag agtacttgga gctttgatgt
     2761 ttataatata tcaaatagga cgtatgtcta tcataatgta tcttccatca gtggttctag
     2821 ctggtcttac aggaatacct gtaaatgtat tgatagtagt tatgggtgta atagcaataa
     2881 tttattcata tacaggtgga ttaaaggctg tcttgtggac agattttata caaggtatgg
     2941 tattaatagt aggtgttaca gggacattgt tctatttaat agcaagtata aatggtggtt
     3001 ttggaacagt aatggatact cttacaactg gacacaagtt cttagcaagt aatgaagtta
     3061 tgtttgataa aaatatacta tctacaagtg catttataat atttattgga gctggtctta
     3121 atactttttc atcatatgta tcgagtcaag atatagttca aagatttact acaacaactg
     3181 atataaagca acttaataaa atgacatatg gaaatggtgt tttatcaatg ggacttgcaa
     3241 cagtatttta tctaattgga acttgtttat ttatatacta tactcaaaac ccagatttag
     3301 ctcaaacagt tcaacaagac caagtatttg catcatatat agcttatgaa ttaccagtgg
     3361 gtataacagg aatactactt gcagcgatat atgcagcttc tcaatcaact ttatcgacag
     3421 ggcttaattc agttgctaca agttggactt tagatataca aactatcata acaaaagata
     3481 taagtatgga aaagcaaact aagatagcac aatatatatc attaggtgta ggtatacttt
     3541 ctatagctgt tgcaatagta ttagcaaatg gagaaataaa gtcagcatat gaatggttta
     3601 atagttttat ggggctagta ctaggtgttt tagcaggaat atttgtactt ggtgcatttt
     3661 gtaaaaaagc gactaaaatg ggagcatatg ttggattcat agtatcagca attttagttg
     3721 tttatttaaa ataccgtatg ccagaagtaa cttcttgggc atattctcta ataacaatca
     3781 cgacttctgt agtggtaggt acaatagtta gtgcaataga agctaaagtt aaagcaaaag
     3841 tatctttacc aaaagctgag actactgttt attatgaaaa ataatattta aatgataaat
     3901 tgtcttatag agtgattaga taaataattt agttactcta taagattgta taatatactt
     3961 tgtatataaa agtataaaaa tagactttat aattatatag ggagatagtg cttatgatat
     4021 ttggaaatat aaatcatagt aagacatatt caacattgca tgaagattta cttagatgtt
     4081 ttgaatatgc taaagataat tcattaatag attatgaaaa aggtacttat acaatagatg
     4141 gtgatgatat ttttgtgaat attgtagagt ataaaacttg tgaaaaagaa gatagattct
     4201 gggaagctca taagaagtat atagatgttc atttgatgct tgatggatgt gaacgaatag
     4261 atataaattt tatagaaaat ttagaacaaa aaatatatga agaaaagggc gatttcttgc
     4321 ctcttgatgg taaaaataat ggatacatgg aacttagaaa aggtgatttt ttagtttgtt
     4381 atccagaaga tgctcatatg acaggaataa aagttttaga aaaagaaaat atcaaaaaag
     4441 ctatttttaa ggttttggtt agataatgat aatttatata accactaggg ctgaaaatag
     4501 tgatttaact ttcagcgatt agtgagactt cgtaaatttt agttagatta ctattttcag
     4561 ccttagtttt ttatgagaaa attgaaaata attaaaagct taaaattttg taaaggagtt
     4621 aacttatgga aaatatagat gtattcttat taataggaca aagcaatgct agaggattgg
     4681 gaaatccaaa agagagcatc attccaaatg aaaattgttt tgaatatttg agtactgatg
     4741 aaattataaa tatgagatgt gagctagaga cttcagaagg tgatggtact atagcacctg
     4801 cattttcaaa tgaatggaat aagcttactg gaaataaagt ttgttttatt cacaatgcaa
     4861 aagatggttc aagaataaaa aactggaatc atgataataa ctggtttcta aatgacacta
     4921 tagaaaaatt taatacaggc tgtactacct taagtaaaca ttataatata gaaaataaat
     4981 acgttatttg gattcaaggt gaaagtgatg caaaatatgg tagtgatgca ttgtactata
     5041 aagaaagtct aaaaaaaatt gcgtataggc ttaaagaaga atgtatgatt gataaaatgt
     5101 ttgtaagttt aactggttat tggcttggag aagatgaata ttttattaga acaagacgaa
     5161 ttgcagcagc tcaagaatct gcttgtaatg aatgtgatat actgtgtgta ggaagtaaaa
     5221 ttgctatgca atttcatgac agaggattaa ctattgatga tgttcattac actcaagaag
     5281 gtcttaatat gttaggagag gacttatgta aaaatattta taagtatcat ataaccaaag
     5341 aaaaaagtgt attaagtgat actatagact tatcagaagc aaggaaatat atctgtgaat
     5401 tggaaaagat aagtaaaaga tttgtgtaat ataaaatttt aaatagagtt gttaatttat
     5461 aaaaataatt tgtatggaga tatgttatga aaaattacgt ttgtatagat gttggaggta
     5521 cttcaataaa atatggtttt attagagaag atggatttat aattgataaa tcaagtcttg
     5581 atactgaagc aaaggaaaaa ggtggagaag gaatattagc caaaataaaa gatatagtaa
     5641 aaaaatatat agaggaaaat gaaataagtg gtatatgtat atcaactgca ggtatggttg
     5701 acccagttga aggtaaaata ctatttgcat tagaagaatt aattccaaat tataagggta
     5761 tgcaattaaa aaaagaagta gaaaaagagt ttaatataaa atgtgaagtt gaaaatgatg
     5821 ttaattgtgc tggacttggt gaagtgtggc ttggcgcagg tagaggtgca acatcttcta
     5881 tatgtttaac aataggtact ggaattggtg gatgtattat aataaacaat aaactgataa
     5941 atgggtttag taatagcgct ggtgaagtag gatatatgaa tataaatgga agttcttttc
     6001 aagaattggc atctactagc agtctaatta aaaaagtagc aaaaataaaa aatttgaatg
     6061 aaaaggactt aaatggaaaa ataatttttg atttagcaaa gaataatgat gaagattgtt
     6121 taaaagaact cgataatatg ataaaatcat tagcagttgg aatcgcaaat atatgttatg
     6181 taataaatcc agaagtggta attttaggtg gtgggattat ggcacaagaa aagttcctaa
     6241 aaccaaaaat agacaaagca ttaaaggagg ttcttataga gagagtttac aaaaacacta
     6301 atatagaatt tgcaaagaga caaaatgatg caggaatgct tggagcatta tataattttt
     6361 taaacaaaat ataaagaata gaagggtttt taatgagtat tttagaacaa ttggaatcac
     6421 caacttttaa agtcacaaaa tcagataaat tattaatagc ttatataaaa gaaaatatag
     6481 atgatgtatt ttataaacca atatcacaaa tagctaaaga gagcaatatt ggtgaagcaa
     6541 ctataactag gtttgtaaaa aaaatgaatt ttaatggatt acaggatttt aaagttactt
     6601 tagcacaaga aatatcaact atcaataaaa aaaatattat aaataaaaat atacaaaatg
     6661 atgagccagc tttggataca gcaaaaaaac ttttaagttc aaatgtaacc actttagaga
     6721 acacagtaga gataatcaat agtaaggatg tacatgactg tgcaaggctt ataataaatg
     6781 caaagaaagt ttacttcata ggcattggat attctggtat aatagcacag gattcaaact
     6841 ataaatttat gagaataggc cttaattgtg taagttttga tagcagtcat acgatgataa
     6901 tgatgtcttc cataatggaa gaaggagatt taataattgc aatttcacat tctggtgaaa
     6961 cagaagagat tataaaaact gtaaagcttg caagagccaa taatgctaaa attatatcaa
     7021 ttactgaaaa taaaaattct gaattaaagg atatttcaga tgtacactta tcttatgtat
     7081 caggagaaac agtactggaa actggttcaa tatcttctaa attagcacaa tttttcatta
     7141 ttgatttagt atatacacag gtagtaaaag aactttctaa tgaagctatt gaaaggaaaa
     7201 ttaaaactac aaatgctata aaattgttta aaaatgaata aaataagaaa tttgtaatgt
     7261 tttatagagc tctcttaaaa atttaggaat ggagagatat tacatgtcta aattaaaaga
     7321 ttataatatt gataaagtaa taatttttaa taatttaagt actaaaacga agaatcaact
     7381 taaagaaaaa gctaaactaa aaagattaca aaaaaaagaa attttatttt atgaaaaaga
     7441 tagcttagac agtgtttata tacttttaga aggaaaggta tctattttta agataagtga
     7501 aaatggagag agaaaagtaa ttttcatatt aaatagtggg gaagtcataa atgatattac
     7561 atttgactct tctaaaaact ccactgttgg ctgtgaagca tttgaaaatt caattgtagc
     7621 ttattatagt attgaagaat ttttagaaat tatgcaaaat gattttcaac ttacaaagga
     7681 tattatttct tatatggaaa gaagaataag acgtttatat agacagttga aaaattctat
     7741 atccataaaa gtagataaaa aacttgcagc aaaattatat aggttaagta gagaatttgg
     7801 agtttgctgt ggagaatgga ctttactaaa tgtaaatatt acagttacat atttagctga
     7861 tatgcttggc tgtaaaaggg agactgtttc cagaatgata aaggtattgc aaaaagaaga
     7921 acttattaag attgataata aaggttttta tgttaaagaa actgaattat caaggtattt
     7981 caaaaataat taattatgat taagtaaaag tattattaga attttaatta aataaaaata
     8041 ttattaagag aatctattta tagattcttt tttattgtct aatttattaa tttttatttt
     8101 accctattga attgtataaa aaagcaaata agattcttta ttattcaaaa aattgagtag
     8161 ataatagttt tattgtattt aatgattgta tctaagtgat tagatttgaa atcgaataat
     8221 atagataata aaaaaatatt aacagagatt agtaaactct attaatattt ttttattatt
     8281 tttgaaactg tgataattat cacagaagca tagaaaataa agtgttaaga tttaaacaag
     8341 atagcaagta agtaaaacta aaatataaga aaggtgataa aaatgaaact aaaattagat
     8401 tcatctaagt tcaatgaagg tctgaattat ctaaaaaaag actacaagat atttgcacca
     8461 gttttaatcc catttaaagg tactttttca gatactgatg ttataagata caaggaggta
     8521 aacgcttttg aagaaatgga atttgctaag aaagcaaact tttcaccaaa agaagttgta
     8581 cttcccataa atcaagtatt gttttatttt actgaaaaag agtttaagga aagcgattta
     8641 gacaacaaga aaattttaat tttcttaaga gcttgtgaca taaatggtat aaaaagactt
     8701 gatgaaatat atctaaataa tggtgaagaa aaagattatt tttataaaaa tatcagagat
     8761 aaagtaaagt ttgcacttgt agggtgtaag gaaagtttta gaaactgttt ttgtgtgagc
     8821 atggattcta acaaaactga taattattca attggattaa atatagaagg agatacactg
     8881 tacttagata taaaggatag tgaatttgaa gtatttaatg gagaagcaac tgaatttaat
     8941 gttgattatg taactgataa tttaatatct gtagatgtgc cagagaatat tgattcaaat
     9001 gaaattatag gaaattcaat atgggatgaa tatgatacaa gatgtatagg atgtggtaga
     9061 tgtaattttg tatgtccaac atgttcatgt tttacaatgc aggatatttt ctataaggaa
     9121 aacgaaaatg taggtgagag aagaagggtt tgggcttctt gtcaagtaga tggatataca
     9181 gatatagcag gtggaaattc ttttagaaaa aaacaaggtg aaagaatgag atttaaaact
     9241 atgcataaaa tacatgattt taaaaaaaga ttcggatatc atatgtgtgt tggttgtgga
     9301 agatgtgatg atgcctgtcc gcaatacata tcattttcaa agtgtataga aaaaataaat
     9361 gatttagtaa ttagtaagga ggaagtataa atgaacccat atataccagt aagtgcaaaa
     9421 atactagata tagttaaaca tactgataaa gaatggactt ttagagtaaa ctgcaataca
     9481 aaaggtgtat tgcctggaaa gttttatgag atatctatac ctaaatacgg tgaaagtcca
     9541 atatcagtat caggatatgg agaggattat attgacttta caataagaaa tgtaggaaaa
     9601 gtaactagcg agttgtttaa ttataaagaa ggagattctt tttttataag aggtccttat
     9661 ggaaatggat ttgatgtatc cctttatgaa ggaagagaaa ttgtagtagt ggcaggagga
     9721 agtgcattag cccctgtaag aggaatagtt gaatattttt ataataataa agaaaaatgc
     9781 gaaaagttta aattaatagt aggatttaaa tcaccaaaag atattttatt tgcagatgat
     9841 ttaaagagat ggagtaaaaa acttgatatt ttagttactg tagatggagc agaggaagga
     9901 tatagtggta atgtaggatt agtaacaaaa tacataccag aattggatat gaaagatata
     9961 aacaatactt ctatagttgt agttggacct ccaatgatga tgaaatttac agtagaagaa
    10021 tttttaaaaa gagatttaga tgaaaagaac atttgggttt cttatgaaag aaaaatgtgt
    10081 tgtggaatag gtaaatgtgg acattgtaaa atggatgata cttacatatg tttagatgga
    10141 ccagtatttg attattcata tgctaaaaat cttattgatt agggggcgaa gtttgtgata
    10201 agagatttaa ataccaaaaa agtaatgaaa aatgcttata gaattacaaa aacaaaatat
    10261 gagacttctt taagagttag agtacctggt ggtcttattg accctgagag tttagctgta
    10321 gtttctaaaa ttgcaagtga gtacggaaat ggacaggtgc acataactac aagacagggg
    10381 tttgaaatat taggaataaa tatggaagat atggaagaaa taaataaaat tatacaacct
    10441 gttattgaaa aaatgaatat aaatcaaagt gagaaaaacg caggttatcc agcagcaggt
    10501 acaagaaata tagcagcttg tataggaaat aaagtctgtc caaaggctca atataataca
    10561 actgaatttg ctaaaagaat agaaaaagct atatttccaa atgatttaca ttttaaagtg
    10621 gctttaactg gttgtccaaa tgattgtata aaagctagaa tgaatgactt tggaattatt
    10681 ggtatggctt taccaatata tgaaaaagat agatgtgtaa attgtggagc atgtgtaaaa
    10741 aaatgttcta aaatatctgt tggagcatta aaaacagaaa ataataaggt ggtaagagat
    10801 aaagataagt gtataggatg tggagagtgt gtactaaatt gcccaacaaa tgcatggact
    10861 agagatgaga aaaaatacta tagattagct ataatgggta gaacaggaaa gaaaaaccct
    10921 agactagcag aagattttct tttatgggta gatgaggaca gcataataaa gataatactt
    10981 aatacttata gatatgtaga aaagtatata gataaagatg caccaggagg aaaagaacat
    11041 ataggatata taattgatag aacaggattt atggaattta aaaaatgggc acttgaaggt
    11101 gtagagcttc cagaaataac taaaatgtac gaaaacatat actggagtgg aattaaatat
    11161 ctataaattg agtaaaacat atatttgaat aaattaaaat ttaatagaag gaagagggaa
    11221 ttatgcataa agaaactttg gataaattaa ctaatgcagc tataaataag ataaatttat
    11281 taaacacaag taaagtaaaa tatttggtat cttctgcgtt tgcaggactt tatgtaggta
    11341 taggtatact tttgatattt actataggag gacttttgac tgatgcaggt tcacctatga
    11401 ctaagatagt aatggggtta tcatttgcaa tagcactaag tttggttata atgacgggaa
    11461 cagagttatt tactggaaac aatatggtta tgtctgctgg tatgttaaat aaaggtgtaa
    11521 gtataaaaga tacttctaaa atatgggcat acagttgggt aggtaattta atcggagctt
    11581 tggttttagg tcttatattt gtaggaactg gtctagtaga caaaggacct gtagctgaat
    11641 tttttgcaaa tacagcagct agtaaagcat ccatgccatt tacagctctt ttctttagag
    11701 ggattttatg taatattcta gtatgtgtat cagtgttgtg ttcgtttaga actaatagtg
    11761 atactgcaaa aataattatg atattcttat gtttatttgc ttttataaca tcaggttttg
    11821 agcatagtgt agcaaatatg acaatttata gtgtttcatt attttcacca acaataagta
    11881 cagttactat tggaggagct atttataatt tagtagctgt aacacttgga aatattgttg
    11941 gaggagcttt atttatgggt cttggaactt atatattagg taaagaaaag cttaattaaa
    12001 tttattgtat aaattataac tctcttgata aatacatttt tagtattatt gagagagtta
    12061 tttttatatt tttgaaaaaa tatttgaaac aaaaaaaata tgggagcata cgaaaatata
    12121 tcggttatat ataaaaaaaa tacgtttata tatgaattat atagttctaa atataaaaaa
    12181 agttaaagtc ctaccgtaaa aaagcgcatt ttgaataaga taacaaatat gataaaattg
    12241 gtcttgttga aatttttagg ggggatatga tgacaaagaa actagtaata tcaaaaacaa
    12301 agttgtttat ttttataata gcatttattt ctata
//
LOCUS       DAEPLF010000092        12308 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEPLF010000092 DAEPLF010000000
VERSION     DAEPLF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.092"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(33..719)
                     /locus_tag="KR314_002179"
     CDS             complement(33..719)
                     /locus_tag="KR314_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBH0328561.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(768..1301)
                     /locus_tag="KR314_002180"
     CDS             complement(768..1301)
                     /locus_tag="KR314_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBH0328562.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDA"
     gene            complement(1345..1977)
                     /locus_tag="KR314_002181"
     CDS             complement(1345..1977)
                     /locus_tag="KR314_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBH0328563.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYNTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(2067..3146)
                     /locus_tag="KR314_002182"
     CDS             complement(2067..3146)
                     /locus_tag="KR314_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBH0328564.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(3279..4625)
                     /gene="pepV"
                     /locus_tag="KR314_002183"
     CDS             complement(3279..4625)
                     /gene="pepV"
                     /locus_tag="KR314_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBH0328565.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(4694..5974)
                     /locus_tag="KR314_002184"
     CDS             complement(4694..5974)
                     /locus_tag="KR314_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBH0328566.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(6101..6361)
                     /gene="hfq"
                     /locus_tag="KR314_002185"
     CDS             complement(6101..6361)
                     /gene="hfq"
                     /locus_tag="KR314_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBH0328567.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(6475..7410)
                     /gene="miaA"
                     /locus_tag="KR314_002186"
     CDS             complement(6475..7410)
                     /gene="miaA"
                     /locus_tag="KR314_002186"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBH0328568.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDIDAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKGKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTARWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(7448..9415)
                     /gene="mutL"
                     /locus_tag="KR314_002187"
     CDS             complement(7448..9415)
                     /gene="mutL"
                     /locus_tag="KR314_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBH0328569.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKDKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(9426..12269)
                     /gene="mutS"
                     /locus_tag="KR314_002188"
     CDS             complement(9426..12269)
                     /gene="mutS"
                     /locus_tag="KR314_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBH0328570.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
BASE COUNT         3773 a         2152 c         1265 g         5118 t
ORIGIN      
        1 caaatcttag gttattattc tttaaattat ttttaaatta ttccaaatac accaaataat
       61 attggtataa atatagctgg taacaaatta gctactttta tattcgttaa tttaagtaca
      121 tttagaccaa gtcctataat taataaactt ccagctgctg tcatttctgt gactacagga
      181 tcacttaaaa atccatttaa aaatgatgct cccacacata taataccttc atacaaaaat
      241 acagatattg cagaaaacat tacacctata cctaaagaag cagtaaatat aattgaagct
      301 attccatcta taacagattt tacaaataaa gtatcatgac ttcctgtaag accactttct
      361 aatgaaccaa ctactgccat ggctcctata cagaataata aactagatga tataaaaccc
      421 tctgctatag aatctttttt atttccttta gaaaatttac tttgtaaaaa agcacccaac
      481 ttatttaccc atttgtcaat atctattaat tctcctatca gagcacctac agctacggat
      541 attattgtca ctaatgtgtt ttttccttct agtgctcctg atataccaat gtacaataca
      601 cataaggcaa ttccattcat tatagtatca cttactcttt gaggtaatcc tccttttatt
      661 ataagaccta ctatacatcc acctgcaatt gctagactat ttgctattac tccaaacatt
      721 ttttctcccc tcgattagct aattgaaatt tatcctatta ttaaaaccta agcatctata
      781 ctttgtattg gatattttaa atattccaaa aattcttcaa aaccttttct agatatattt
      841 acagttttag tatttatgtt aggatgaaaa tttaagtaat cttctttaat tacatcttta
      901 tctatgtaaa gctcaacttt tctttctgta tcatttatta atgaaaaagg atttacactt
      961 ccaggaagaa gtttaagtac attgtatagt ctttcaggtg aagcaaacga taatctagaa
     1021 cttcctatct tatctttaag tgagtttaaa tcaacttgtt tttctccttt aactacaata
     1081 agataatact tatttccttt ggcatttctt aaaaataaat ttttacattg acaccccttt
     1141 gttattccat ctaaaatgac taattgctct gctgtatata cagcttcatg ctctactaca
     1201 tcatattcaa catttaattt atccaaaata tcatatattt tagtttcatt catgctagat
     1261 tcattttcat cttgttcttt tttattattt aaatcactca tacttttctc cttaaacact
     1321 tcatcataat atcattgtga ggtattattc tactatagct attgctctta ctggagctcc
     1381 atcagcatca ttatacttaa atggtgctgc tataaataga aatttttctg gaacattttc
     1441 taaatttgta agattttcta tgattatttt tcctttttca aataaaatat gatgcacttc
     1501 aaatactgat gtgttatatc tatccactga tagcatatct attcctatgc cttttatatt
     1561 agtattagct atatagtttg cagcttcttt tgttaatgta ggataaccta catagtactg
     1621 ctttttatcc caaaattttg accaacctga tttaaatatt ataaaatcac tattttttat
     1681 ttcatcttca tattttttta gatattctaa ttctatattc tcatttccct tttcaagttc
     1741 caatacataa gctttcccaa caaaattttc tatatccaac ttatctaggg tctcaccttt
     1801 agtatacata tgtcttggtg aatccatatg agttccattg tgagaaaata cactaattaa
     1861 tgtttcttga taaccatttt cttcaataat agctactttc ttgatatctg gtctattagg
     1921 ctctgcatat acaggcatgt cattatgtgt cacatgagtt aaatcaaaaa ccttcatctt
     1981 tatatacccc ctatgttttt attttctatt atatacctaa attcatttta tttctatcag
     2041 attttattcc attatatacc atactactat atatttttta taaacctcaa gggatttacc
     2101 gaaaaatctt ggaaagaatt atcagtaaat ataacatctt cacccaaaaa acgtttatac
     2161 cttccttctt ctttctttaa agtaattctc tgtttttgtg aatgattatt aaaaaccatt
     2221 atcttaagag gaggtttttt tctattagtc tgcactcttt ttaacatgta ttttatgtgc
     2281 atatcagctt caggaaatga atatccacaa aatattaaaa ggtcagtatc tcttaaagtt
     2341 ttttctgttt cattccaaac attacttaaa aatatgtttg acatattctt aaaatatgtt
     2401 ggtggcacta taataggttc tgttaactca ccacactcta aacattttgt ctctgaacta
     2461 ttttcaataa gcttcatgac accaccttca tgtggtgtaa gagttacact attacaaatg
     2521 gggcaataaa gccaattcaa tgacccatgt atcttatata atttgattaa tggttctttt
     2581 ggttttctcc aatttaactc ctctttaaaa tttgtaaact ccacaccata atcaagcata
     2641 attggcaaat cattcttatc tcttaatcca gtaactgtat tgtctatatg tatatcataa
     2701 tttgcactaa taaaagtagt atcaagaagc aaatcatttt ccaaaagatt ttcaactaaa
     2761 agtttatgat atttattatc tgttctaggt gtattatgaa ttgcttcagc cattagcagt
     2821 attaaatatt gtcttaaaaa acgtatacta tcacttctgt tatttagatt ttctagtcca
     2881 aaatttctaa atgcttcttt tctttgttct gctaagtcta aaagacctaa aacttcttca
     2941 aaggtaggaa aatttgcatt atctatatta tcttttacta tatcaatatt gaacatctgt
     3001 ttaaagaaaa tatacaattc tctattcatt ttggtattat aatttgtggg atttagtttt
     3061 ttaaaatatt cgctaaaaag ttcattttga ataggtagat tctctgctgc tgaagctcct
     3121 gctcctaaaa atattgctat tttcatatag ttcctcctaa tataaatatt actcctattt
     3181 ttatatccat taaatatcca tatcatttat tttatgatac aacaataaag ctgtctttta
     3241 ctagaaaaaa tcctattaca agacagcctt atattatatt aaatctttaa taattcgtaa
     3301 atagaaagag catatatttg agtagctttt ataatatcct ctactattaa gtattcatta
     3361 gcttggtgtt ctgtttctgg ttttcctgga aaaatagctc caaatgcaac acaattatct
     3421 aaagctcttg cgtatgttgc tcctccagat gaaagtggtg ttccatcaag accagtttct
     3481 tcttcatata cttttctaag tgtcttaaca agaagagtat cttctggaac atagattgaa
     3541 tccaagaaat catattcttc ataatttaaa ttgtatttat ctgtcatttt ctttaactct
     3601 tttacaaaat catccttttt gtatgttact ggaatcctta catctattcc tgcaaattct
     3661 ttttcgttat caatagttac tctacctata tttactgtta gtttacctga tacatcatcc
     3721 ttgcaattag gaattatatt gtttccatta gcatcttcac caataacttc tgctaaaaac
     3781 ttaattatat tagaatctat acctatatta ttaagagcta tacaaagtct agccactgca
     3841 tttataccag tatcacttgc tgctgaatgg acactttttc caattataca gattttatta
     3901 ccttcaacag tatactcaaa atttaattta tcaagttctt tttttaattt atcagagtat
     3961 tttccaatat agattgcttt acctggaact gcatttaaag cctttccaac tgacaactct
     4021 atatcacttt tgctatcaca agtcaaatga acttgtaaaa gacctttctc tgcatttgtt
     4081 attgggaatc tagaatctgg agtaaatcca tagtttggta tttcttcatt attttcttta
     4141 tatttattta tacatctcca aagattttct tcatcagccc caaaaatgaa tcttatcctc
     4201 ttattaaagt ctacatttaa atccataaga gctttcactg cataaattgc tgatattgta
     4261 ggtcctttat catcttgtgt tcctcttcca tacaatttac catcttctaa aacagcttca
     4321 aatggagggt aattccagct ttctaaatca ccttctggaa ccacatcaac gtgaccaagt
     4381 atacctatca ttttttcacc ttgtcctatt tctgcatatc cataatatcc atctttgtat
     4441 actgtcttaa atcctaaaga tttacataag tctaatatac ctcttaaagc tttatctaca
     4501 ttttcaccaa atgggcaatt ttcagttgct tcagatataa cactaggtat ttttacactt
     4561 tcttgtattg aagaaatcat ttcatcttgt agagaattta ctttttcttt aatttgctct
     4621 ttcatattat cacccttcaa ttttcatcat aatatactaa taaatatttt gaccttttta
     4681 aaaaaaaata tgcttatttt ttcagttttt ttataaattc aatcgctttt aaagtcccca
     4741 tttttgaatg gtcaaatgta agtccacctt gaaaataaac attataagga ggtcttattg
     4801 gagcatctgc acttaattct atagaagaac cttgtataaa tgctcctgct gccattatga
     4861 cttcactttc gtatccaggc atgtcccaag gaacaggttt tacataagaa tcgactggtg
     4921 cagcttcttg tataccttca caaaaactta ttacttcatc agcattatta agtctaatac
     4981 attgaataat atcacttctt aagtcatcat attttggtaa tacatcatat cctaattttt
     5041 caaaagctct tgagcaaaag atagctccca ttacagcctg agatactata taaggcgcca
     5101 agaaaaaacc ctgaagtaca tttctagttg ttccaaaagt aagaccacat tcttttccta
     5161 ttccaggaga agtcattcta taagatataa gctcaatcaa atcttttcta ccagctatat
     5221 atccacctgt caatgcaagc ccaccaccag gattttttat taaagagcca gccataacat
     5281 ctgctccaac atcagtaggc tctttggtat ctaaaaactc accataacaa ttatcaacca
     5341 ttactatagc ttctggttta acagacttta ttacttctat agcttctttt atatcactaa
     5401 ttgaaagtga ttttctccaa gaatatcctt ttgacctttg tatcataacg agtttagttc
     5461 tatcatttat tttgttttta attccttcta aatctaagtt tccatcctct aaaaaatcta
     5521 catcatcata tgtaacacca tactctttca atgaaccttt ttcttctctt atgcctataa
     5581 ctccctctaa agtatcatat ggtctaccag taacagataa aatttcatct cctggtctaa
     5641 ctataccttg tatacataaa gtaagtgcat gtgtaccatt tacaattatt ggtcttacaa
     5701 gagcatcttc tgtattaaat actttagaat aaatttcctc tattttttca cgaccaatat
     5761 cattatatcc atatcctgta gtccagttaa agtgcatatc acttagttta gattcttgca
     5821 tagcttttaa aactttatat tgattatatt ctcttatttc ttttatttct tcaaacttat
     5881 cttttatttc ttccattatt tcttgcgata gtttaaaagt atcatcatct attccatagt
     5941 agtcttttaa taattctttt gtctcattaa gcattttatc acctgtctct ttattatttt
     6001 atttaaacat gtaaaggaga ctattttact aaaacaaata aattatagtt tataagttag
     6061 tataagtctc ctctcaatca tcttaaatta aattaattat ctatctgttg ttattattat
     6121 tgttgttttg gttttgattt gttagattaa tatactttcc aggttgtata gttgatacag
     6181 catgtttata aatcatgttt tgttgtctat tatccccttc tattaatatt atataactat
     6241 caaacccctt tacaagccct ttaacttgca ctccattaac taaatatata gtaacaggta
     6301 tcctttcttt ccttgcatta tttaaaaaca aatcttgtaa atttaaaact gtatttttca
     6361 ttagatttgt accctcccaa gattaaattg aacttctaaa atattcctac cttatattct
     6421 atataagtta tgacaaattt tcaatattaa gttgtatact taaaaattta tatattattt
     6481 tatagaatct ttaatatata agattatttc attttttaat tcgtctatat tttcatattg
     6541 gtctaaatca aaccatcttg ctgttttata tcttttaaac catgttatct gacgttttgc
     6601 ataccttcta gaatctcttt taattatttc aatagcttct tcgtatgtat attctccatc
     6661 cagatacttc aatatctctt tataacctat accttgcatt gatatcatat cttttttaaa
     6721 tcccatactt aaaagttttt ttacctcttc aaccaatcca tttttaatca taatatcaac
     6781 tctcatattt attctttgat acaaatgttc tctatctcta tttagaacta ttattattgg
     6841 ttgatattct tcattaaatt ttaaatcact tgaaaaatca ttcattttct taccacttag
     6901 acaaacttca agagctctta tgaccctttt agtattgttt ttatggattc tattagcaga
     6961 ttcttcatca agttctttta acttattatg catgtaatct attccctttt cagcaagctg
     7021 tttttgaagt tcttctctta attcattatt ggcatcagat tgtgcaaaat ccatattata
     7081 tattaaagag tttaaataaa gtcctgtgcc tccagttact aaaacacatt ttcccttttc
     7141 atttatttca tgtatatatt tttttgctct ttgttgaaac tctgaaactg aaaatggata
     7201 gtcaggctta acttcatcta taaggtaatg cttcactcct tgcatttctt cctctgtaac
     7261 ttttgcactt ccaatatcca tatattcata tatttgcata gaatcagctg aaattatctc
     7321 tgcatctata tcttttgcta attttattga caaatctgtt tttccaacag cagttggacc
     7381 tgtaagtatt ataagtggta tttttttcat aacattcctt tccatatatt ttaaatatcc
     7441 aaatatttta cataattctt ttaaacattt tttctatttc tgtcttagat atttcgacca
     7501 ttataggtct tccatgaggg catgtaaatg gattctcaca cttttctagc tgttccaata
     7561 aactttttat ttcaatatcg tatattttat catttgcttt tatagcagac ctacatgcca
     7621 tagatgcaaa tctctctcct tttaaatcat aattacttgt aatttcttct atattatcaa
     7681 ttatttgaag tataaatttc tcagtttcag gtactccaaa tatagtagga acacatctta
     7741 ccattatatg attattgcca aaaatttcta gttcaaaacc aaatttcata aataactcca
     7801 agttattttc aatttgaagc atatctacat ttgatacttc aataaccact gggtctaaaa
     7861 gtatttgcat attgatatct tgtctataga acttctccat atatctttca tatagtattc
     7921 tttcatgtgc agcatgttgg tccaacaaat acatagaatc gtctttgctc aaaattatat
     7981 aagtattaaa tacaactcca attacagaat atccatataa ggaaaacttg cgttttgaac
     8041 tacacaagta ttcttcttca ctgtccttaa tactatctcc taagtaataa tttccttcat
     8101 ttttaattcc atcaacttga aactcttctt gtatattatc atttgcacta tcttcactta
     8161 gataattaat gttttttaca tcatcttcta cacttgcatt taggacttca cttactgatt
     8221 gaaattcatt tattggtttt tcacttgata aagtcacaac ttcaatgact tcatccttat
     8281 ctttagtaat attactattt ttaggagtag attctaataa atcattattt cttaatttat
     8341 aatctgtact tttttctcta ctgttaatag cgattctagg ttgtgttttt ttatcactat
     8401 aagtagcata ttttccaata agatttgagt gtattaattt aacctttaaa aaatctctaa
     8461 gttctatata cacttcttgt tctttttcaa atttaatttc aagtttattg gggtgtatat
     8521 ttacatctat acatgatggg tctacttcta tatttaagaa gcacactgca tgtttaccta
     8581 ttggaattat agacttataa ctttctgtta ttgcatctat gattatttta ctttttacaa
     8641 atcttttatt tatatagata tgttgtaagt ttttatttga acggtatata ttgttattac
     8701 ctatatatcc attcatttta aagtgattgc acttaaactc tacatctatt atattttccg
     8761 ttatttcttt tccatatata cttctaatag tgtttacaag ttttccatcc cctggagtat
     8821 ttaacatctg tttattgtta tttgtatatt taaattgaat gtttggatta cctattgcaa
     8881 gtttatttat caaatcactt atgtttatag tttctgcatg agtagatttt aaaaatttct
     8941 gtcttgctgg tgtattaaag aaaatatctt ttataatgat ggtagttcca ttagtagaac
     9001 ctattggttc ttttgatatt atttttccgc cttcaacata tattttagtt cctattattt
     9061 catctttagt tttagtcgtc atttctaatt ttgatacagc tgaaatagat gccaatgctt
     9121 ctcctctaaa accaagagag tataaatcat acaaatcatc aattttttta attttactag
     9181 tagcatgtct caaaaatgat ttttcaactt cacttgaagg tattcctatt ccattatctg
     9241 taatcttaat tagacttttt cctccatcaa ttatatctat ggaaatttta tttgcacctg
     9301 catcaatgga gttttcaata agttctttta ctacagaaga aggtctttct acaacttcac
     9361 ctgctgctat cttatttata gtcaaatcat ctaggatgtt aattatattt ttcattttta
     9421 cacctctaag attttatttc ttttgctttt ctttgaagat tataaagaga gtttatagca
     9481 tctaatggag tcatatttaa aatatccaga tttaaaattt cttctgatag tatatctcta
     9541 tttacactat caaaagagat ttgagttaag gctacttctt ttacagaatc ttcttgatta
     9601 attttctctt tatcatcatt ttgcttcatt aaagcaactt catcatttaa tgctttaaat
     9661 ttagaattta atttatcata atcctttttt atatgtttgt agtcttttac tataagttca
     9721 tgttctattt ttagagattc atatttattc ttaaaattac tttgatttac cttaactaat
     9781 tcttcgtcca aattactatt aattatatta ttgttttctt tatctccatt tattgcaaca
     9841 ctgttgtaaa catgattttt ttccaagtct ttaagtatat attttgctct ttctatgact
     9901 tcgtctggta atttagctaa tttagcaaca tatattccat aactcttgtc tgctccctga
     9961 ggaattattt ttcttaaaaa tattattcct tccccatctt ctttgactgc tattgaataa
    10021 tttttaacct ctttaaactc ttcttctaaa tcagtaagtt catgataatg tgttgcaaat
    10081 aatgtcttac atctaatatt tttttgaata tattccacta ttgaccatgc taaacttatc
    10141 ccatcatagg tactagttcc acgacctatt tcatctaata taactaaact tctctctgtt
    10201 gcatttttta atataaggga tacttcattc atctctacca taaaagtaga ttgaccttga
    10261 gataaatcgt cactagctcc aactcttgta aatattctat ctaatattgg aatatcagca
    10321 gactctgctg gtacaaatga gcctatatgt gccataagag ctattatagc agtttgtctc
    10381 atatatgttg atttacctga catattagga ccagttatta tgttaataat gttttctcca
    10441 cgatttaaat aagtatcatt tggaacgaaa ttctcctctc ctactatatt ttcgacaaca
    10501 ggatgtctac catttcttat atcaagttta ttattttcat ttattgctgg ttttacatag
    10561 ttatttatat gtgcaactgt tgcaagggac acaaaaacat ctatattagc tatagtctta
    10621 gccacttttt gtattctatc tatgttttta tatattgtat ctcttatctc tacaaatatc
    10681 tcgtattcta aagatttaat cttttcctct gcatgtagta tcttttcttc tatttccttt
    10741 aattctggag ttatatatct ttctgcatta ctaagtgttt gctttcttat atatgtctca
    10801 tctagctttg cctgtttaaa gttggctttt gttatttcta tataatatcc aaaaacttta
    10861 ttaaatccta tttttaatga tttaactcct gttttttctc tttccctgtt ttctatttcc
    10921 tttacaagaa aggctccatt cttagaaatc tctcttagtt ccttaagttc atcactaaaa
    10981 tctgatttaa ttatgtttcc atcttttatg gttatcgtag gttcttctag aatagcttca
    11041 tctattaaat tgtatatatc atctaattta tccatttctg aaacatactc ttttaggatt
    11101 tttgcatttg ataaatttat agtatctttt aaattaggta acttttctat cgaatttttt
    11161 aagtgaatta actcttttgg tgttactctc tcaaatgcta tctttccaca tattctttct
    11221 atatcgtata tatttttaag tatatcattt aggtcttctc ttaatataaa gtcatctttt
    11281 atttcttcta ttacatctaa tctattttca attttagatt tatttatcaa aggttcttct
    11341 acatattttc ttaaaagtct acctcccatt gctgtagaag ttttatcaag tacatgtaat
    11401 aatgaacctt ttttcttatt ccctcttata gtttgagtaa gttctaaatt agttctagta
    11461 aacatatcta aaaccatata ttctgatgaa ttgtatatat ttatattatt aatgttagat
    11521 gttatttgtt tttgagtatt gtatatataa ttcagaagaa ttgataagct tgactttatt
    11581 agacctttat catcaaactt tagtttttgt agataaacat ctggaaaata ttcttttaat
    11641 atatttatat ccaaatagtt atcactaaaa ctttcattta tgtatatatt acttacagta
    11701 gctatatctc taagcttttc tataaagtct aaatcattta taattatttc agttggatgg
    11761 atttttgcaa tttcttcaat taccttatct tcatttaaac atgttgcatt agtttctcct
    11821 gtacttatat caacataagt tagacctata ttagtaccat ccttatacaa agataataaa
    11881 taattattct ttttattttc aagtaaattt ccatctaaaa ctgtaccagg agtgatgact
    11941 cttataacct ctcttctaac aattcctttt gctgtagatg ggtcttccat ttgttcacct
    12001 attgctactt tatatccatt ttcaactaat tttgatatat atgaattggc tgagtggaaa
    12061 ggaacaccac acataggagc acgttcttct aaaccacaag cttttcctgt caatgctatt
    12121 tcaagagctt ttgaagctac taatgcatct tcaaaaaaca tttcataaaa atctccaagt
    12181 ctaaaaaaca gtatacagtc tttatatcta ttttttactt caagatactg tttcatcata
    12241 ggagttaatt tattcatatc tatttgcatt atcaaacctc cttcttttct aattaaatat
    12301 tatatcat
//
LOCUS       DAEPLF010000093        12206 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEPLF010000093 DAEPLF010000000
VERSION     DAEPLF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.093"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            71..1060
                     /locus_tag="KR314_002189"
     CDS             71..1060
                     /locus_tag="KR314_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBH0328571.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIIFLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     misc_binding    1212..1464
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1536..2744
                     /locus_tag="KR314_002190"
     CDS             1536..2744
                     /locus_tag="KR314_002190"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBH0328572.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     gene            complement(2805..3686)
                     /locus_tag="KR314_002191"
     CDS             complement(2805..3686)
                     /locus_tag="KR314_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBH0328573.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPGRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            4072..5016
                     /locus_tag="KR314_002192"
     CDS             4072..5016
                     /locus_tag="KR314_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBH0328574.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLI
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            5239..5784
                     /locus_tag="KR314_002193"
     CDS             5239..5784
                     /locus_tag="KR314_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBH0328575.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            5875..7023
                     /locus_tag="KR314_002194"
     CDS             5875..7023
                     /locus_tag="KR314_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBH0328576.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQNSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSKKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            7076..8341
                     /locus_tag="KR314_002195"
     CDS             7076..8341
                     /locus_tag="KR314_002195"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBH0328577.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(8627..9859)
                     /locus_tag="KR314_002196"
     CDS             complement(8627..9859)
                     /locus_tag="KR314_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0328578.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(9862..10536)
                     /locus_tag="KR314_002197"
     CDS             complement(9862..10536)
                     /locus_tag="KR314_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328579.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLVN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            complement(10544..11773)
                     /locus_tag="KR314_002198"
     CDS             complement(10544..11773)
                     /locus_tag="KR314_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBH0328580.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            complement(11885..>12206)
                     /locus_tag="KR314_002199"
     CDS             complement(11885..>12206)
                     /locus_tag="KR314_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0328581.1"
                     /translation="YFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIK
                     KEDLNRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITF
                     KCDL"
BASE COUNT         4414 a         1535 c         1998 g         4259 t
ORIGIN      
        1 ttatatttat taaaaatatt tttaaaaagt gatatactat attaaaaaca aaactttagt
       61 tagaaaattt atgatttatg gaggcagtgt tttgaatact actaaaaaat taactgaagc
      121 agctttattg tcatctttat tcatagtagt aacaattata gctgtgagta ctggatttgg
      181 atatgctatt tacttagatt ttatagtgcc agtatttttt tgtataatct gcttgaaatg
      241 tgatttaaag tataccattt tatctggaat tacttcactt ttaataattt ttctggtact
      301 tggaaatcta ggaactgcta tttgggcaag tcagagtgta ttacttggta taatgtgtgg
      361 ctatctaata aataagccta caatggttat ggatgattta gtatatggtt cagtatttgg
      421 ggttattgta atggttttca ttgatatata tgcttcaaca ttaataggtt atagttttat
      481 gaaagaattt caaggatata gcaaatggat ttattttaat gggtatacaa attttattta
      541 ttatttaatg atagcattgt ttccttttgg tatggtgttt tgcatatatt tattaagttt
      601 aattttaggg aataaattgc atattttaga ttctaattct ttaaaaaaat ttttaatatt
      661 taagaatttt agaagtttaa accaattttt atgttgctca aaaaaagctt tttacatatg
      721 tgtttcttat ctagccattt ttgaagttct aaaactgttg aatgttaaag ttgattctgt
      781 ttatttaaaa acaatcttta tatcaataac ctatataagt gtctatttta tcattagaga
      841 ctcatgtatg tcactacaaa actttataat agctaagttt agaaaattat tatatgctag
      901 aattttattt ttgataataa ttttattgct gttttttata tttgatgtaa cagttattag
      961 tttaataatt ataaacgcat ttttaaataa gaaaataaat ataagattga gtcagagtaa
     1021 tatagtcaat aaacaaataa atcttttgat tgaaaaataa actttgggta ttggtaaaaa
     1081 atataaaaaa gtagtataat gtaaaaaaac atgtatataa taataaaaaa ggttgacaaa
     1141 atataaaaat aataatatat tatgagtaag ttaagaaaaa tttcttgtaa ttgatgaaat
     1201 aatctgaata tataaatgct atgaagagaa gaagtagggc tagattttat ttaacagaga
     1261 gttccagttt ggtgaaatgg aataaattga tttagtttga atacatctca gagcactatc
     1321 tctgaaagat aacaaccatt aagagatatc ggattagtac acgttatagt acacgagtat
     1381 atgtaattgt actctaagag attaatatcg tgagatattg atgaactaag gtggtaacac
     1441 gtaagcaatg ctttcgtcct tttaagagga tgaaagcttt ttttattata aaaaaaaaat
     1501 tatagattat ataaatattt taggaggaat gaaaaatgaa atcgatagac gagcaaatgc
     1561 gaataattat gaaaggtgta gatgatttaa tagatgagaa agagttaagg gaaaagctta
     1621 ttaaatcaga aaaagaagga aagcctatga tagtaaaatt gggtctagac ccaagtgctc
     1681 cagatataca tttagggcat acagtagttc ttagaaagat gaaacaatta caagatttag
     1741 gtcatcaaat agtaataata attggagatt ttacaggaaa aattggagac ccaactggaa
     1801 aatctaaagc tagaaaagct ttaacaacag agcaagttct tgcaaatgca aaaacttatg
     1861 aagaacaaat tttcaaagtt ttagataaag aaaaaactat tgtaagattt aatagcgagt
     1921 ggttagccaa gttaaatttt gaagatgtaa taaaattagc agcaactatt actgttgcga
     1981 gaatgttaga aagagaagat tttaaaaaga gatatgaagg acaaatgcca atatcagtac
     2041 atgagttctt ctacccatta atgcaagcat atgactctat agcattagaa gctgatatag
     2101 aacttggtgg aactgaccaa agattcaatt tactaatggg aagatcatta caaagagaat
     2161 ttggtatgga gtctcaaata gtaataatga tgcctttaat agaaggatta gatggtaaag
     2221 aaaaaatgag taagagttta ggtaactata ttggaataga tgaagaagcg ggaataatgt
     2281 accaaaaatc tatggaaatt cctgatgaac ttataattaa atactacaat ttagtgacag
     2341 atgttcatcc tgatgaagtt aataaaatag aatctcagtt gaaagagggt tcagtaaatc
     2401 caagagatat aaagatgaac ttagcaagag aaatagtaac attataccat ggagaagaat
     2461 ctgctaaaga agctgaagaa agatttaaat ctgtattcca aaaaggtcaa atacctgaag
     2521 atatacaaac aattcaagtt aaggaagatg gatttgactt aatcgaagta ctagtttcta
     2581 atgagattgt taagagtaag agtgaagtta gaagattagc ttctcaaggt ggagtaaagg
     2641 taaatggtga aaaagtagag gatttaagca ctattgtaaa agagagtgaa ttagttgttc
     2701 aaataggaaa gaaaaaattt gtaaaaatcg agctagtaaa atagagtaaa gtaaaagcaa
     2761 gtctattgag tataaaatgt acattcaata aacttgcttt tttattatat taaaatttta
     2821 aattgataac cattgtcttt aaaatatgtt ataatttggt ccaatgcatt tactgtttct
     2881 tgttttccat aagtatcatg cattaaaaga acaaccattt ctttgccttc agaagatttc
     2941 tttgcatgtt caaataattc ttgtgcattt ttctttttac cttctgcatc agcatttaaa
     3001 gcgttccaat caattgatgc catgtttttc tcttttaagt agtttcctag aggttccata
     3061 tttttccatg acatataccc gcctgggcat ctaactacat ttgaggaaaa attctttcct
     3121 aagacttttt tcatagcttc atcagttttg ttaagttcat ttacgaatgt atcgagattt
     3181 aggcttctgc caggatatag ttttttatag tcatgggtat aagagtgatg ggctattgca
     3241 tttccttcgt caaatgttct ttttaaaatt tcattagctc cttttctttc aagagaatca
     3301 cccttaataa agaaagttcc tctaacacca tgtcttttca agatatctag aacttgtgga
     3361 gtattagtag tagatggtcc atcatcaaat gtaagaaaga ctatcttttc tccattgttt
     3421 gaatagtcgt atttgttaag tttgtcccaa actatttttg cgtcatatcc atattctttt
     3481 acagcttctt tttttgcacc aataataggt tcattttttt ctttttcaat tctttctttt
     3541 tctaatcttt cagctttaag tctttcttga gtttctttca tttctttttt tctagacatg
     3601 tccgcccatg ccatagaaaa aataccaata actattacaa caagtatagt aagtgtaaca
     3661 gttttctttt ttcttttttt taccataatt acagcattcc cccttttgtc gaattgtatc
     3721 aaaaatatat tataattata tcgaagaaag tagaaaaagt aaaccgacaa agttgtaact
     3781 aagataacta aagtgtaact tatatgacat tatttgcata atattaaagt atcatatata
     3841 ttaaaaatat ctataaaaaa atttagtata ggtaaaaaaa tataaaaaat ataaaaattt
     3901 attaaaaagg tgtttacaaa aaatgattaa tatattactg ttaaatagtg gatataaatt
     3961 tgagctcaaa atgtatgtaa atttagattt aaaatactga aaaaatttac tatattaatt
     4021 tatagtaatg cttatagaaa aactctcttg ctgtttaggg ggtttataat tttgcaaaaa
     4081 aataaaaagg agggggcttt attgaaaaaa tactttggag aaataggact aatatttata
     4141 gcaattatat ggggaagtgg ctttgtagca acccaatttg cattagacgg tgggcttaca
     4201 cctctacaaa tcataacttt aagatttttc ttggcagcta taattatgaa tttactgttt
     4261 tttaaacaga ttcgtgccaa tatgggtaaa aaattattaa aagcaggagg gattttagga
     4321 atatttttat ttctagcatt cacagtacaa actattggac ttatgtacac tacaccatct
     4381 aaaaatgcat ttataacagc agctaatgtt gtaatagttc cgttcatagg atttatactt
     4441 tatagaagaa aacttgataa aataggaata ataagtagtt tggtagctct tataggaatt
     4501 gggatacttt ctttagaagc tgatttctct attaattttg gagattttct aacacttata
     4561 tgttcattcg gatttgcatt tcatatattc tttacaagtg agtttgcaaa agataacaac
     4621 ccaatggcat tgacagctat tcaatttaca gtagcgtttt taatgtctgt tgtagttcaa
     4681 acatttgcag gtcaattaaa gatggaagct gaattatctg gttatatggg aactatgtat
     4741 ctagctgtat ttagtactac aattggattt ttatttcaga ctatatgtca aaaaagagtt
     4801 gatggaacta gaacagctat aatattatcc acagaggctg tatttggtac aatattttct
     4861 ataataattc taaaggaact tattacagca aaattgatta ttggtagtat cttgatattt
     4921 gttgcaatca taactgcaga aactaaatta tcttttttaa agagcaaaaa agtgaaactt
     4981 aaagattcag aggagtcttc attagaaagt atttaatatg acaactattt cctaattaat
     5041 caaataagtt atgtattatg tagaaatttg acatcaaaac ttggttgata ctggtgtttc
     5101 aaagtgataa aattaattag gaaataaatt ttatttttag tgtttaagct ataaagtctg
     5161 gggtatatat attcttgtag caagttatta aacttaaaaa taaaaattta attatataaa
     5221 attgggagga aattaattat gaaaaaattt gtttgtacag tatgtggata tatacatgaa
     5281 ggagatgctg caccagcaca atgtccagta tgtaaagttg gagctgataa atttgaagaa
     5341 atgaaaggcg aaatggtttg ggctgatgaa catagaatag gagtagctca aggtgtagat
     5401 gcagaaataa tcgaaggatt aagagctaac tttactggtg agtgtacaga agtaggaatg
     5461 tatttagcaa tgagtagaca agctgataga gaaggttatc cagaagtagc tgaagcatat
     5521 aagagaatag cttttgaaga ggctgaacat gctgctaaat ttgcagaact tcttggagaa
     5581 gttgtagttg cagatacaaa agaaaactta agagttagag ttgatgctga gtatggtgca
     5641 actgatggaa aattaaaatt agctaagaga gctaaagaat taggattaga tgctatacat
     5701 gatacagtac atgaaatgtg taaagacgaa gctagacatg gtaaagcatt cttaggatta
     5761 ttaaacagac attttggaaa ataataatta aaacatagat taaaaggaga aactttggtt
     5821 tctccttttt taaattatac aaataaactg ttgtgataga aggatggaga taatatgaag
     5881 tcggtatctg ttaggagttg taaaacttac aattatgaag aagtaaagtt atctattgaa
     5941 aaaaatttag aggatattgg tggtatagat aagtttgtta aaaaaggaag caaagtactt
     6001 ataaaaccaa atttattaat gaaaaaaaag cctgaagaag ctacaactac acatcctatg
     6061 gttgtaaaag ttgtatgtga aaaactttta gagttaaatt gtgatattac aatagcagat
     6121 agccctggtg gtccatataa tcaaaattct cttaagagca tttataaggc atctggaatt
     6181 gaagaagtag caaatgaact gggcattaag ttaaattatg atgtttcaga tgttaagata
     6241 catagtaaaa aagcacatgt tctaaaatat atggatatta ttaaaccaat agtagattca
     6301 gattatataa taaatctttg taaattaaag actcatataa tggcaacctt tactggtggt
     6361 acaaaaaact tatatggatg catagcagga ttgaaaaaag cagagataca ttatagattt
     6421 cctacagaag agttgttttg cgaaaatgta cttttagata tttgtgatta tataaaacca
     6481 acattgacaa ttatggatgg tatagtaggt atggaaggtg atggaccttc agctggtact
     6541 actcgagaaa taggagtttt attagcatca caaaatccat atgctataga tgttgttgca
     6601 tgtaagatta taagtttact tccaaataga gttgctacaa ttagaggtgc tattaagaga
     6661 ggctatatac aagaagattt ttcagatatt gaaattttag gtgaagatat aaaagattta
     6721 attataaaaa attacaagat tccaaatgta agtaaggatt taagattgtt ttccgtaaag
     6781 ctaccaaaat ttttaaatga acctatttca aagcttataa caccaaagcc tgtggtaaga
     6841 tttcaagatt gcataaaatg tggaaaatgc aaggaagctt gtcctgcatc cactatagag
     6901 atagggacta aaggtgctat tatagactta agtaaatgta taagatgtta ctgttgccat
     6961 gaactttgtc ctaaaaaggc tattgatgtc aaacgtaatt ttgtatttaa gtatgtaaaa
     7021 taatgtataa tattatatga gtctatataa attaaaaata taaaggagaa ataatatgat
     7081 tttaattaaa aatggtagag taatagaccc taaaagtcaa agagatgaga atattgactt
     7141 aattataaaa gaaaataaaa tttataaaat aggcaagttt gatgaaagtg atgaatatga
     7201 aaaaataata gatgctagtg gtaatgtagt tgctccaggt ttagtggatg tacatgtaca
     7261 ctttagagac cctgggttta catataaaga agatatagaa agtggagcta aatctgctgc
     7321 tagaggtgga tttacaactg ttatatgtat ggcaaatact aatcctattg tagataatga
     7381 agatactttt aattatgtta aagaaaagtc aaaaaatgca tgtataaatg ttttacaagc
     7441 agcagctata actaaaggtt ttgaaggtaa agagttagtt gatatggaag ctcttaaaaa
     7501 agctggtgta ccagggttta ctgatgatgg attaccttta atggatagca atctaattat
     7561 ggaggcaatg attaaggcaa aagagttgga tgtaccactt agttttcatg aagaagaccc
     7621 ttctttagtt ggaaatccag gtgtaaatgc aggaaaggta gcttcagaac taggattaaa
     7681 aggtgcatca agtgtagcag aagatgttat ggtagccaga gattgtatgt tggcactaaa
     7741 gactggtgct aaagttgata tacaacatat aagttcaggt gtatctgttg atatggttag
     7801 gtttgcaaaa tacttaggtg caaatgtagt tgcagaagct tcaccacatc actttacatt
     7861 gactgaggaa gatgtattgg agtttggaac aaatgcaaaa atgaatccac ctctaagaag
     7921 taaatgggac agagataaga ttatagaggg attaaatgat ggtactattg aaataatagc
     7981 aacggaccat gcacctcata gtaaagaaga aaaagataga gagtttataa aagctccaag
     8041 tggaattatt gggcttgaaa cttctttagc tcttggaata acaaatttgg tgcataaaaa
     8101 tcatcttagt atgatgcagt taatagaaaa aatgtctata aatcctgcaa agttgtacaa
     8161 tttaaatatt ggatttatag aagaaggggc tgtagcagac attgttatat tcaatccaga
     8221 agaagaatgg acagttgaaa gctttgtttc taaagctgat aattcaccgt ttaaaggtaa
     8281 atctttatat ggaaaggtta attatacaat atgtaatggg gagattgtat ataacgccta
     8341 atttaagatt atttttttaa ggcggagtgt acaatgattg aaagtaaaag attgtatatc
     8401 agcagaatga gattgattaa tcaaaattgg aagtattagt ttgaacaatt agaacataac
     8461 atcttggtaa tttagatata gtaaggaaga gaatcctttt tatgaaagaa cgcactatta
     8521 tatttggaaa gattaagttc ctaatctatc atatataata gtgcgttata tatttaagta
     8581 tgttttatat aaactaatct aaaaatcgaa aatctttata caatttctac ttataaattg
     8641 cctttatagg gtctagttta gaagctttaa aggctggaat cagaccaaat acaactccag
     8701 tcaatatagt agctacaatt gcataaaata agctatttaa gctaggtata gcttcaaaac
     8761 ctatatattt agaaacatag ttagtagcag caaatccaac tatagttcct aaaattccac
     8821 cacatactgt tataaaaaca gcttcaacta aaaattgaaa taatatagac ctaggttttg
     8881 ctccaatagc acgtcttata ccaatttctc tttgtctttc cataacagat acatacatta
     8941 tattcattac accaatacca ccaacaaaca ttgcaactac agttattata gatacatatt
     9001 tgtttacatt ggaattaatg ctttcaagat aagctgtctg ttcagttggg tctggtgtag
     9061 tataagaacc attgatacca gggtgcatct catataattt agctataaca ttgtttgcaa
     9121 cttcatttac attatagcct ttagaagaaa ctaagtctaa ctggtaaatt tcatttgaat
     9181 aagaattttg actcattaaa gtgtcaaaag cctttttagg tactagggaa gtagtaaatt
     9241 gcatatcttg gtaacctcca aagaatcctc cagcttggtt ttgctgagaa tcatctaata
     9301 cccctattat ttcaaatata gttccattta tgttgatacc atgacctaag gcatcttctg
     9361 gattttcaaa aatttcactt gtactttgta atgtaagtaa aattactttt cttttttcat
     9421 catctaaaga aaaatctcta ccacaaatta aatttatttt agaatctttt tttactggtc
     9481 caacatcaac atacgtagtt tttttatcaa aagatgcttg tgaagagtaa actgaatcaa
     9541 ggttaaaccc atctcttgaa ggagctattc tttctactcc ttcaacaaat gataattctt
     9601 ctaaatcttt tgcattaaag ggttttaaaa aaatactcat atcatcagta agacctgtgt
     9661 tatcggctga ttcaaacgaa atagtagttt tattagggtt tacattgttt acagatttct
     9721 taatttcttt ttgaaatcca ttacctatag aactaacaag tattactgaa gttattccta
     9781 ttataatcca gagtaaagct acaaatacac gaagcttgtg accttttaaa ttggccaaag
     9841 aattttttaa taaactcatg attacacctc cgaagtaaac acaccatctt taagtcgtat
     9901 aaccttagtt gcatacttag ttaaatcttg atcatgtgta accattatta tagtcttacc
     9961 ttgtttgttg agccttgtta aaagctccat aatttctctg ctagtttcac tatctaatgc
    10021 cccagtcggt tcatcagcaa agattatctg agggtcattt actaggcaac gtgcaatggc
    10081 tacacgctgt tgttgaccac cagataattg aagtggtttc tgcttaagct tgtccaaaag
    10141 cccaacagaa cttaatttat ctttaacaat ttcttctcta ttagattttt ttagtgcttt
    10201 attgtaaatt agaggcaact ctacattttg atatacattt agagtttcta ttaaattaaa
    10261 ttgttggaaa acaaatccaa tattaatatt tctaaaaaca gaacgttcat tttcactcaa
    10321 attagttaca tctgttccat caaatatata tcttccttca tcaaatacat ctagaaaacc
    10381 taagatatta agtaaagtag ttttaccact accagactta cccattatca tgacaaattc
    10441 tccagattca atttctaaat ttaaggattt taaaacatgt aattcatctt tccctacttt
    10501 gtaatatttt tgaatatttt ctaatttaat taacatatgt aaattaattc acatccatag
    10561 taacttcttc tacttttttg tttggagtag tatctccatt attagaacca gcagttacgc
    10621 catcgccttg aactgggtct ccttctttca tttctttagt agggtgtttt attattatat
    10681 cgccttgttc aagaccgcct ctaactacag caaaatcatc attttgactt gctacatcta
    10741 caatttgctt tttcaatata ccatctaaat ccttaaatac atatgagtgt ttgttgtctt
    10801 taagtacaca agatgctggt attttaaagc ttgaattagc tacttcaagt gatgcttgaa
    10861 gatggaatcc atttactaat ccttcttgat tatcaaatgc tatgtttata tcataataag
    10921 atagtgtatt ctgctgagct cccatttcag tatttggagt agatggttta tcagatataa
    10981 aacttattct accagtaagc ttttggtcag ttgaaaaaac tagtatattg accatttggt
    11041 caatcttcat tttaggtaaa tcttgttcac ttgcttgtcc tttcatataa aactctagac
    11101 cttgaatagt cataaatgaa actggttggt ctggatttcc agtttggtca ttcaagtata
    11161 ctgtgccatc aaaaggagca gtaactctgt cataagcttt tttatccaga gaagctatct
    11221 gagagtttaa agcagttatc tgtgcattta tagaagcaat agcatcttta ttttcagcaa
    11281 tatcagaaag tgatattttt tgttttttgt attgactaag ttgtgatttt aaactatcta
    11341 tttctgctat tatagttgga tttttagcag taaaaagaag gtctccttga tttacttttt
    11401 gaccatttgt aacattaaca tcacttaact cataatctcc agatatattg aagtccttag
    11461 tttgcttagg aacaaccatc ccatttataa atattttttc attttcagga atagtatatg
    11521 tttcgataaa attttcatta gcttgtaatt cagtgttttt cgaatcaaca taagcaagaa
    11581 ctcctagaat taaaagtatg attactgcta ttcctgcata tattttgaat ctcttatttt
    11641 taaagggatt gtttttaaaa cctttatttt tagacttgaa gccactactt ccaaaactgc
    11701 tatttctaaa gttgttactt ttaaaactgt tatttctaaa tcttttattt ttaaattttt
    11761 ttagaaaaga catgtcttcc tcctttttat aaaaactact taattatata ctgggatgta
    11821 catatagaat tactataaga agttgaatat aagttaatga aaataaacta tcaataataa
    11881 gatactataa atcacatttg aatgtaatag taaattttgt atattggttg tactctgaat
    11941 ctacatcaat tttatgacca agcttatcac aaagttcttt tactaaataa agcccaagac
    12001 ctgtagattt agaattgttt cttccattat tacctgtaaa tcccttattg aatattcgat
    12061 ttaaatcttc cttttttata ccacaaccag tatcttccac tcttaattgt aaataatttt
    12121 tggttctttt gcaatagata gatatattat ctcctaatga ggagtatttt agggaattac
    12181 tcaataattg ctcgaatatg aagtat
//
LOCUS       DAEPLF010000094        12092 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEPLF010000094 DAEPLF010000000
VERSION     DAEPLF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12092
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.094"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(19..924)
                     /locus_tag="KR314_002200"
     CDS             complement(19..924)
                     /locus_tag="KR314_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0328582.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKGVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            1286..2020
                     /locus_tag="KR314_002201"
     CDS             1286..2020
                     /locus_tag="KR314_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328583.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSVVIEKSDHEKSAVSLENFR
                     RIDLGEYVGVEESRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            2231..3412
                     /locus_tag="KR314_002202"
     CDS             2231..3412
                     /locus_tag="KR314_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBH0328584.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGEVFNKVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMIILNVKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGILGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGIHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVEIVI
                     R"
     gene            3417..3968
                     /gene="mobB"
                     /locus_tag="KR314_002203"
     CDS             3417..3968
                     /gene="mobB"
                     /locus_tag="KR314_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBH0328585.1"
                     /translation="MKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGYRVGVIKH
                     TCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQLYEDMDLI
                     IIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLNDYKNISKY
                     IESCIKNEILSISNVEIKNILKE"
     gene            4087..4362
                     /locus_tag="KR314_002204"
     CDS             4087..4362
                     /locus_tag="KR314_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0328586.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIHAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            4618..6318
                     /locus_tag="KR314_002205"
     CDS             4618..6318
                     /locus_tag="KR314_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328587.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            6681..7868
                     /locus_tag="KR314_002206"
     CDS             6681..7868
                     /locus_tag="KR314_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328588.1"
                     /translation="MEAITAFCLVLISLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTIVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            7928..9091
                     /locus_tag="KR314_002207"
     CDS             7928..9091
                     /locus_tag="KR314_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0328589.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IEIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            9242..10045
                     /gene="zupT"
                     /locus_tag="KR314_002208"
     CDS             9242..10045
                     /gene="zupT"
                     /locus_tag="KR314_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBH0328590.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            10141..10701
                     /locus_tag="KR314_002209"
     CDS             10141..10701
                     /locus_tag="KR314_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBH0328591.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            10952..11665
                     /gene="rgbR"
                     /locus_tag="KR314_002210"
     CDS             10952..11665
                     /gene="rgbR"
                     /locus_tag="KR314_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBH0328592.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDDEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNAAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
BASE COUNT         4721 a         1205 c         2090 g         4076 t
ORIGIN      
        1 actactttat taaagttatt atattataat ttcgaaatac tcacataatc tgtttagaaa
       61 tttatcttct aatggggaaa aagttttctt tttagagtaa ataaagaaaa attttctagt
      121 aaaatcaaca ccctttattt tataacattt tattttacct gcattgagat aatcaatagc
      181 tgaaatgtac gatataaagg agactccaag acctaatcta accatttcct taatagcctc
      241 atttgattct acatgagcta ttacatttaa tttacttact ggaaagttat ttttactaag
      301 tgtgtttagt attaagtttc ttgtacctga cccttctttt ctcataataa aatttagata
      361 agccaattct cctatatcga tacaattatt tttattatca attttaaaat ctgaaggagt
      421 gataagaaca agttcatcat ctaataagtc taggtactct atttgaggat tattgatttt
      481 agaaccaact aatccaaagc ttattcgttc atttaaaatt tctgatatag catattgaga
      541 atcataatgg cttatggaaa attttacatc tggataactc atagaaaaac tcttcataaa
      601 atcaggtaaa atataagttt caggtatgga actacatgct atatctataa taccttctat
      661 ttttcctgaa tattctttta tatcataaat tgcacgtttg caattattca atatatatat
      721 agcatgttca taaagaattt caccagattt tgtgagtgta ataactttgt tgcttctatt
      781 tataagaaca gtttccaact ctctctctaa attctgtatg tgtgcactta cagtaggttg
      841 agttaaaaaa agttctcttg cggcctttga aaaactctga tgttttgcaa cagcaacaaa
      901 tacttcaagt tgtttaaaat ccattattta atcctcctta aattatctaa aactattata
      961 acatatttga taaattaata gtcaaaggtg atttttaaaa tagaaaacct ttaaatattc
     1021 atggagtttt tgattgttta ggaataattt ggtataatat tgtaaggtac ttacaataaa
     1081 aatatcgtaa attgaatttt caaaattcaa ctttaaagtg ttttttatta cagaaaaatt
     1141 acaattagct tcttaatctt attaaattca aataaatatt atataattat tttataatat
     1201 aataaatata attattgaaa aaagagataa ctgtaaattg agatatagat aaatactata
     1261 aaatttaaga aaggaggatt gatttatgaa gttaaaaagg tcattagttt gtgtaactat
     1321 tttaggtatt atacttgttg gatgtcataa agaaaatact aaagagaaaa atcaagtagc
     1381 aagtaaagca acacagcaaa aaactatgac aaaagttcaa aatgatgtta atgaaattat
     1441 gaataaagat tataagtaca ttataaagaa tatgggtata ccttataata cattttatta
     1501 tataaaacct aaagttttaa aagaatcgaa tacaatgcaa gatataaata catcaagtta
     1561 tatgacatta gtttatccaa aatatactgg aaatgatgaa ctagatggaa gtgctttata
     1621 tgttgatatt aacgaaaata aagttgtcaa tgttgaaacc aattctttct catcacaagg
     1681 tatatcagtc attgatgctg aatctagtgt tgtgattgaa aaaagtgacc atgaaaaatc
     1741 ggctgtatca ctagaaaatt ttagacgtat tgacctggga gagtatgttg gtgttgaaga
     1801 gtctagaata aatgaaattg taggagatgc gaattatgat ttaacagcat ataatcatga
     1861 aggtagtaaa gtagttaaga gttatagatt gaaagaagat aacaaaatat taaaaaaaga
     1921 ggtacttaca ataagtattg tggataacaa aataaaatct ataaaaacta ttgaaagtga
     1981 taaaattgta aaaataataa aaggaacttt attagaataa aatattctaa agcatatgat
     2041 gctgtttcaa gatagaagga aatttctagc tgggaacagt ttttttatta gatttataaa
     2101 atttgaactg cacttataga taactattag ttataattaa atataagata aaagtatttc
     2161 caacttttat aatctataat atattacata gaaatatctt aaataaaaaa tatattgggg
     2221 agtatttaaa atgatagaat tagaagatgc cataaaaatt attgaaaaaa atgttttacc
     2281 aataaacagg gtcaagatag taaatcttat agatgcagta aataaagtag ttgcagaaga
     2341 tatatattca acaattgata gtcctccatt tgataggtca ccctatgatg gttatgcata
     2401 taatgcaaat tctgaaaata gaaatttaaa ggttataggg actttgtatg caggagaagt
     2461 atttaataag gtattaaata aaaatgaagc agtgaagata atgactggtg gaaagatacc
     2521 acagggtgca aactgtgtaa taaaaaaaga agatgtaacg gttataggtg atatgataat
     2581 tttaaatgta aaattaaagg aatacgataa ttacatattt aagggtgagg atataaaaaa
     2641 ggggcaattg attattaata aaaatcagaa tataggatat gatggtattg gagtattagc
     2701 aagtcttgga atatccaaag taactgttta tgatgattta aaagttggaa ttttgaatac
     2761 aggtacagaa ctacaagata taaatgagac tttagagaat ggtaaaatat acgatagtaa
     2821 tagatatacc ttatattcaa gattattaaa acttggaata caggcagata caatatgcaa
     2881 ttgttctgat gatatattgg agttagaaaa aaaggtaaag aatatgttac agagtgtaga
     2941 ttttttaatt acaacaggtg gtgtttctgt tggagataaa gatttaattc cagatgtatt
     3001 caaaaaaata ggggcaacag aattattttg gaagataaac attaaacctg gtggagcttg
     3061 ttatgtaggt atattgggag aaaagctatt atttggtctt tctggtaatc cacatgcagc
     3121 aatagtggta tttgataatg tgatagttcc tgttattgag tatttaacat ataagaaggg
     3181 aatacatcat ataaaagcta tatttaaagg agaatttaat aaactttcaa ataaagatag
     3241 atttgtaagt ggaaaactat atacaagtga aggtaaattg tatgtagagc taaatgataa
     3301 gaaagatgca aaagctagat tatctgcaac tctaagaagt aattgtatga ttaagattaa
     3361 aaagaatgag acaataaaag aaaatcaatt agtggagatt gttattagat agttttatga
     3421 aaggacacaa ttttatgtat aatatattgt cagttgtctc atattcagga gtaggtaaaa
     3481 ctacgttgat tgaaggcata ataaaagaat taaataaaaa agggtatcgt gttggagtta
     3541 ttaaacatac ctgccatgat tttgatatgg atgaggaagg aaaagatact tataaacata
     3601 gaaaatctgg ggcgagaaaa gtctgtataa tatcagataa acgagtagca tatattgaag
     3661 aactaaaaga gaagaatcca ttatataaaa tgattcaatt atatgaagat atggacttga
     3721 ttatcataga aggatataaa aattatagat ttaaaagatt ggaagtaact agaaaaggta
     3781 agtatgagga tattttatct aataagtctg atttgatagg tattgtcagt gatatagaat
     3841 ataaattgaa tattgaacag tttaatttaa atgattataa aaatatatca aagtacatag
     3901 agtcttgtat aaaaaatgaa attttaagta taagcaatgt tgaaataaaa aacatattaa
     3961 aagaataatt ttaagatgtt cttcttatat aaaatgatat aattatttat ataaataaag
     4021 atataagaaa tttatcaatt ttattttgag ttataaaata ttaaagaaat tgtaggagga
     4081 caatatatga aaaaaatatt agtagcatgt ggagcaggaa tcgcaacatc aacaatagtt
     4141 tgtgacagag tagaaagatt ggttaaagaa aataatatac acgcagaagt tgagcaatgt
     4201 aaaattgctg aatgttcaac aaaacaagaa ggtgctgatt taatcgtatc tactactata
     4261 ctaccaacaa cttatgatat acctacaata aaggcaacag gatatataac aggtataaat
     4321 acaacagcat tagataaaaa gatattaaat gcattgaagt agttttgtgt ttataaaaaa
     4381 aatttagtat taaatataga gttgtatctt tataaaattt taaatataga gatatagctc
     4441 tttttatgct gtttcaagta tacaactata tgatataaat cttcaatttt atgaaaaaga
     4501 ttgttattac tttgtaagtt tataaaagat ttaaagttat tgttatgtta tactaattta
     4561 tgggtatttt ttagaagtac tatatagaag tactaataaa ggagggattt aatacttatg
     4621 ataaaacaat ttgtcaaata ttataagcca tataagaaaa tttttacact agaccttata
     4681 gcagcttttc tattttcctt atgtgattta gtttatccta tgataactag aaatataatg
     4741 gatgacgttg ttccaaataa aaacttaaga atgttagtag tttttgcagt agcacttata
     4801 cttatattta tagcaaaagc agggcttaac tattttatgc agtattgggg acatgttatt
     4861 ggtgtagata tgcaagctga tatgagaaat gaggtattta cacacttaca aagacttcca
     4921 aatacgtatt ttgataataa caaatctgga gtaactatgt ctagaatagt aaatgattta
     4981 atggatataa cagaacttgc acatcatggt cctgaagatt tattcatttc tatagtaatg
     5041 ttggttggtt cattctttat acttatagat ataaatattc ctcttacgct tataatattt
     5101 gctatattac cgtttataat ttggtttgca atagctaaaa aagataagat gaatattgct
     5161 tttatgaaga gtagagttac tataggtgat gtaaatgcta ctttagaaaa tagtatagca
     5221 ggtatgaaag taactaagtc tttttgtact gagaaagaag agttaaataa gtttgtaaga
     5281 agcaataaat tatttagaag agcaagacaa gattcttata aggtaatggc agaatattac
     5341 tctggtatga atttatacat ggatatctta gagtgggtag ttgtaatagc tggtggttat
     5401 tttacttata taggaaaaat tactttgggt gattttgctg cttatatact atatgtaaaa
     5461 atgtttatac aacctatgaa aaaattaata aattttacag agcaatatca aaatggtatg
     5521 acaggtttta aaagattcat agagataatg gaacaagacc atcaaaaaga agctaaaaat
     5581 cctattgaac ttgaaaatgt aaaaggtgac attgagatag aaaatatatc tttcacatat
     5641 gaagataaga ctcaagtttt ggataattta agtttatcta taaaagctgg taagactata
     5701 gcattagttg gaccttctgg aggaggaaag actacattat gtaatctatt gccaagattt
     5761 tatgaatttg ataagggtga tataaaaatt gatggaaaga gtataaaaga tgttagttta
     5821 aaatcattga gaaaaaatat aggtatagtt caacaagatg tatttttatt tacaggaact
     5881 ataagagata atattctttg tgggaaccct aatgctacag atgaagaaat gatagctgct
     5941 gctaaaaaag ctagaataca tgactttgta gagactttgc cagatggata tgatacttat
     6001 ataggagaaa gaggcgtaaa attatcaggt gggcaaaaac aaagaatttc aatttcaaga
     6061 atctttttaa agaatcctcc aataataata ttggatgagg ctacatctgc tttagacaat
     6121 gtaacagaaa gagaaattca agagtcttta gaggaattaa gtaaagatag aacaaattta
     6181 gttgttgcac atagattgac tactataaaa aatgctgatg aaatcatagt attgacggat
     6241 aaaggaattg aagaaagagg tactcatgaa gagttagtaa acaaaaatgg agtatatagt
     6301 aggttacaca ataactagta tacaaatttt acaccatagc taacatacgc attaaactta
     6361 caaacatagg atatattaag attcataaag acaattcatt ttatatttat gagatgtttt
     6421 atgaataaaa tgtatcctat gttttatttt ttgcaaatta catttaattc tctttgtaag
     6481 aatagagtta aatagccaat ggtcaggata acgtttttac aaaaagaata atttagaaaa
     6541 ctaactttaa tttatggaat ttatcgaatt ttatgaaaaa aatcgaattt tgacttgaaa
     6601 tgtttttcaa aagtagtaaa ataatggata actatacata cattttataa aaatacattt
     6661 attatgaggg gggattttgt atggaggcaa taacagcttt ttgtctagtt ttaatatcac
     6721 tgacgatagg agatatagtt tcagcaaaaa ctaaagcttt tgttccgtcg gtttttgtat
     6781 cagcaattat atttatagta ggattctgga cttttttccc agaaaatatt gtggacttag
     6841 ccgctttagg aacaccatta gctcaattag gtatgctttt gttaataact catatgggaa
     6901 ctatgatgag tattaaggaa ttagcaggtc aatggaaaac aatagtaata gccttggcag
     6961 gaatagtagg aatatgtgta ggtgctttgg cattagggac aatagtattt ggatgggata
     7021 cagcagtaat agctacacct cctcttactg gagggctagt tgcatctata atgatggcag
     7081 atgctgctac agcaaaaggt cttgcaagtt tatcagtact tgcaattttg atgtatgttg
     7141 cacaaggatt tgcgggttat cctattacag cattaatgct taaaaaagaa ggaaaaagac
     7201 ttttatcaga ctttagaagt ggaaaagtaa ctgtaaatac agatgaagaa aaagttaagg
     7261 atttacctga acaaaaaagc agatttaaga taattccaga cttaccagaa aaatatgata
     7321 cgacatatat gataatactt agattaggat ttgtagcatg gttagcagta caatttacaa
     7381 atcttacaaa tgaagttgta tcaagatatg ttatatgttt aatatttggt gtaatagcat
     7441 cagaacttgg aatagttgat agaaaaccac ttaataaatc tggttcattt ggatggttaa
     7501 tgacatcatt gatggctttt atatttgcag gattagcaaa agctacacca gcaatgcttg
     7561 ttgaaatggc agttcctctt gtaggaatca taatatttgg tgtaataggt atgggaatat
     7621 tctctattat agtaggtaag cttttaggtg aaagtaaaga aatggctttt tcagtttcgc
     7681 ttacatcact ttatggtttt ccaccaaact atatattaac tgaagaggca gtaaaggctt
     7741 tggctgaaac accagaagaa aaagaatttt taatggataa gatgcttcca aagatgcttg
     7801 ttggaggatt tactacagtt acaatagctt ctgtagtaat agcaggtatc tttgtaaatc
     7861 taatatagta tttaagtgat aaaaagtatt ttaaataaga agaatgtgta ctacttggag
     7921 gtatggaatg gatattaaag aaataacaaa gagttataaa gactatgtaa ttaagcttag
     7981 aagggaattt catgaaaatc ctgagaaaag tatggaagaa gttagaacat caaagagagt
     8041 taaagaagaa cttgataaaa tagagatacc atatgtatca gctggaggta caggtgtaat
     8101 tgcaactata aaaggagcta atccaggaaa gactgtagca cttaggggtg atatggatgc
     8161 tctacaagtt gttgaatgca ctgatgtaga atacaaatct aaaaatgaag gacttatgca
     8221 tgcttgtggt catgatggtc acacatcaat gcttctaggg gcagcaaaag tgttaaatga
     8281 tattaaggat agtatcaatg gaactgtaaa attatttttt cagccaggag aagaagttgg
     8341 taaaggtgca agagctatga ttcaagatgg tgctatggaa ggtgtagata gtgtgtttgg
     8401 aatacatctt tggactgatg tagaatctgg gactatatcg gtagaagaag gaccaagaat
     8461 ggcttctgcc gatttcttta aaattactgt aaaaggtaga ggtggtcatg gttcacttcc
     8521 tcatcaaggt gttgatgcag tgcttgcaag ttcagcaata gttatgaatc tacaatctat
     8581 ggttagtaga gaagtaagtc ctctggaacc attagttgtg agtgttggag ttttaaattc
     8641 tggaactaga tttaacgtaa ttgcgagtga agctgtctta gaaggtacaa taaggctatt
     8701 taatccagag cttagaaagc aaattcctgg aattttagaa cgtatagcaa aatctacagc
     8761 agaagcatat agagcagatg ctgaattaga gtatgggtac ttaacaccag cagttataaa
     8821 tgacaaagaa tgttcaaaga tagctacaga tgcagcaatt aagctatttg gagaagactg
     8881 tataacacta tttgaaaaag taacaggagc agaagattta gctgagttta tgaatatagc
     8941 acctggtgca ctagcatttg taggagctag aaatgagtca aaaggtgctt gctatcctca
     9001 tcatcatgga tgctttaata tagacgaaga tgctctagaa attggaactg ctttatatgt
     9061 gcaatatgca gtggatttct taaataaata attttaatat actataaaga aaaagcagag
     9121 actaagttta tatctctgct tttttattgt gtatattttt tataaatatt gactatataa
     9181 aatatatatg atagcataaa gaaaatgata atcaatatca attaaaaaga aggtgattta
     9241 tttgaataat gtaatatttg catttttgat aacattattg gctggtttat ctactggtat
     9301 aggaagttgt atagcatttt ttgctaaaaa aacaaatact aagtttcttt ctataagctt
     9361 aggattttct gctggtgtta tgatttatgt ttctatgata gaaatttttc ctaaagcaca
     9421 agatgctttg acaaaatcta tgggagagaa attaggaagt tggtcaactg taatagcttt
     9481 ttttataggt atggctatca ttgctgttat tgataagtta attccacaag aagaaaatcc
     9541 acatgaaata aagaaaatgg agaatataga agaaaagaat atcaaaaaaa ataagtctct
     9601 tttgagaaca ggtatattta cagcaatggc tatagcaatt cacaattttc ctgaaggatt
     9661 agctacattt atatcagctc ttgatgatgt tacaatagct atcccgatag caattgcaat
     9721 tgcaatacac aatataccag aaggtatatc tgtatctgta ccagtttatt atgccacagg
     9781 agataaaaag aaagcttttt attattcctt tttatctggt atgtctgagc ctttaggagc
     9841 gattattgga tatgtacttc ttagaaattt tttaaatgat ataactcttg gaattgtatt
     9901 tgcaattgta ggaggaatta tggtattcat atcactagat gaattattgc catcagctag
     9961 agagtacggg gaacatcatt tatctattta tggattgatt gcaggaatgg gtgttatggc
    10021 tattagttta ttactattta aatagctttt taaatatatg attgtaacct aaaattagta
    10081 tcaaaatgat ataattattt aaatagattt ttaagctatt taaaaatatc attttggaga
    10141 atgagtgcta tggaaaagaa aataaaattc aaatttgact ggtatagttc acctgataag
    10201 atagagaaat ggttagaaga tattgaagct aaaggatata atttatataa gataaataaa
    10261 tttggaggaa tattttattt tcataaggga agtccaagaa aaataaaata cactgttgat
    10321 tactactatg atgtagagaa taattatttt gatgaacata aatcaaatgg ctggaatctt
    10381 gtatttagga gtattggtag ttttatgggt acatctgcat ggtatatatg gtcgaaagaa
    10441 tacaaagaca tacctcctaa attatatagt gattctgata aaaaaaacaa agtaaagtca
    10501 attttattta tgaatatgtt tatattcttt atgttagtag taatatttat atatttattg
    10561 aatattgaaa taagaaattt atatagtggt tatggagcat ctgtaactgc tattgtacta
    10621 cagattatat tgttgatttt atatgcagta caattatata ggagattaat gtattattta
    10681 aggcttaaaa agtttaaata aataaaagaa ggtgtcctaa aattattttt ttaatatttt
    10741 aagacatctt cttttgttag taaagtactg tttttaatta tagtaatttc tgtctttaaa
    10801 ttgaaatatc aaataatagt tgtattggat tgagtattga taaaaataat ttaaaaatgt
    10861 tatcctaaag tatcagatat acaaaattat ctttaaatat attataaata ttttaattgt
    10921 attaactaga aattatgtaa ggaggcattt tatgattaag atagcagttt gtgaagatga
    10981 aaaagaaaca caacttttaa tagaagatta ccttgagaat atattaaaag atataagtat
    11041 agaatatgag atacaaaagt atatatctgg agaagagtta ctggaaagta atttaaaaga
    11101 tatagatata ttacttcttg atataaaaat ggaaaaatta aatggaatgg atactgcaag
    11161 aaagattaga gaagtagacg atgaaatgga aataatattt gtaacttcat taatagatta
    11221 tgtgcaagaa ggttatgagg taagagctta taggtattta ctaaagccaa tagagttgga
    11281 agaactaaaa aaacatgtgc taacttgtat caaagatatt gaaataaata aggaaagtca
    11341 tattacaata aaaaataagt ctaatacata taagatttat ttaaatgaaa taaaatatat
    11401 agaagttcaa aaaaaagata tgctaataca cacaataaac aagaattttg atataaaata
    11461 tagtttaggt aaaatagaaa aagaattaaa tccatataaa tttataagat gccataaaag
    11521 ttttatagta aatttaagat atgttgaaaa tataaagcct aatgctgcaa tactggaaag
    11581 tggagaagag gtacctatta gtagatatag atataaagaa gttaaggaga aatttttaaa
    11641 atttcttggt gatacaatat gctaaatttt atgaatgagc tattagtagt tttatctata
    11701 tttatacagt tttctataat gaacggaatt ttaatttata aaaaggatag taaaaaaaat
    11761 gaaaaaatag tattctatat tgtcttgttt cttatttata ttataattca gataagtaga
    11821 ttatttaatg gaggaagtta ctttagtaat ataaactatt tattttatat attagcattt
    11881 attgcttggg gaggatacta taattatttt tataagatgg attatattaa gtattccatt
    11941 ttcttttatc tatttagtgt atattataaa tgtttggatg ctgttatata tagaatacta
    12001 ttttttgcaa ttacaggtaa tgttatttca tctaaagaat ccttattagt acctcactta
    12061 gaatctataa aaattcagat atatagtaat tt
//
LOCUS       DAEPLF010000095        12045 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEPLF010000095 DAEPLF010000000
VERSION     DAEPLF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12045
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.095"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(55..1011)
                     /locus_tag="KR314_002211"
     CDS             complement(55..1011)
                     /locus_tag="KR314_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0328593.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDNNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSYGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIQFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENKSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            1714..2709
                     /locus_tag="KR314_002212"
     CDS             1714..2709
                     /locus_tag="KR314_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0328594.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVLKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            2687..3550
                     /locus_tag="KR314_002213"
     CDS             2687..3550
                     /locus_tag="KR314_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBH0328595.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            3547..4341
                     /locus_tag="KR314_002214"
     CDS             3547..4341
                     /locus_tag="KR314_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBH0328596.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISKLNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            4626..6005
                     /locus_tag="KR314_002215"
     CDS             4626..6005
                     /locus_tag="KR314_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0328597.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKELLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            6460..7173
                     /locus_tag="KR314_002216"
     CDS             6460..7173
                     /locus_tag="KR314_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0328598.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSAVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(7445..8983)
                     /locus_tag="KR314_002217"
     CDS             complement(7445..8983)
                     /locus_tag="KR314_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0328599.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVEEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            9866..10321
                     /locus_tag="KR314_002218"
     CDS             9866..10321
                     /locus_tag="KR314_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBH0328600.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            10370..10954
                     /locus_tag="KR314_002219"
     CDS             10370..10954
                     /locus_tag="KR314_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBH0328601.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSREQMDEVKLAMGFKL"
     gene            complement(10959..11282)
                     /locus_tag="KR314_002220"
     CDS             complement(10959..11282)
                     /locus_tag="KR314_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0328602.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLESL"
     gene            11434..11952
                     /locus_tag="KR314_002221"
     CDS             11434..11952
                     /locus_tag="KR314_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0328603.1"
                     /translation="MNFVELTKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGRQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
BASE COUNT         4319 a         1401 c         1826 g         4499 t
ORIGIN      
        1 tagtatatag gacaagatac tctaataaat ttgcctattt taatatcttt tgtactaaaa
       61 ccaactaaag tttcctttat tatttcgttt tgaataatac aaagcataat ccgattgttt
      121 gtataaagac tggaaagtat cagatttatt ttctaattta gatataccta tagaacaaga
      181 attactctct agacataatt ctaaaacttt ctttgcttta ttttcaacat tctcttttga
      241 ttgattacta tattcaaaaa cagcaaattc atctccacca agtcttccaa taatagcatc
      301 atttacgaat actttagtca aaatatttga aaactgtatt agtatttcat ctcctcgaac
      361 atgaccaaag atatcattta cctgtttaaa attatctaaa tcaaagatgt aaaagtatcc
      421 atatgaaaca gttttaagca tttcttttat gtgtacttca aaaaaatgtc tattgtatag
      481 tccagtcata aggtcttttt ctgattttag tttaaattca tttgccattg ctagtgcttc
      541 ttgtgctttt tcagttattg ttttagggta atactcatct ttcatttggt caaagtttgg
      601 aatagaatgg tgaacatgga taagtttaat catatcatct gtaactctgt ataccatagt
      661 aaatcgagta tccatttcta catagatatc tttttctagg taatcttttt ctattaccca
      721 aagagtacca tatactataa aagcttcgtc tgtaactttg gtacatgaat accactcatc
      781 tataatctca aagtctattt gctctgattc tgtttggtct ttttcaagac ctataataaa
      841 ttcttctaat gtttcgtaaa attcatgttt tcctgttcca attaatgcaa ggttgttatc
      901 taataaggaa ataataaatt taaggttttc ttcttctgga tttaaaatat aaatatgcca
      961 aatttttttt gataaatctc ttaattgttt ttgtattgta ttattttcca tagattaccc
     1021 ccttaagatt tacagtttat attttactgt attataagtt gaactcttat tacaatatta
     1081 ttataatata gaactaaaga aattacatcc tgtataggta aaagtatatc ttttacaata
     1141 aaaatattac agtaaaataa ccagttttaa tatacgtatt atatttttaa tttataaacg
     1201 aatattataa atgaaattta aattcaaccc tgaagaaata agctaaatgt attagtatat
     1261 aaatccatac ttgaacataa ataaccttta tgatatgata tcaataataa tattatgaga
     1321 ggagtgcctt gatgtcggag ttatataatg gtaaccaaca attaaatagt tggagacaca
     1381 tatattctgt ataatcagaa attttaagtg tatttttaat acatttaatt tttgtgtctt
     1441 attctgcctt tatagataac tgacatatga caacaaatag tatttaaaat atatattact
     1501 ttgttaaatt atggtgtagt aggctttaat ctgctacact tttattaatt ttgtatagta
     1561 ttattttgat aactattttg aaatagagag cattaatttt tctagtccac aggagttttc
     1621 tgtttttgtg ctgttaaata taagcacaga aaaaggcaga tatatacctg tggacttttt
     1681 ttatataaat tatgaaatat ggaggtagtt aaaatgagta taattgaact taatgaactg
     1741 actaaatctt atagaagatt tgagaaaaag gcaggattta tggggagtgt aaagtcttta
     1801 tttaatagga aatatacaga aaaaacagct atatcgtcat ttgatttaaa gatagaagaa
     1861 ggagaatttg ttggattaat aggtcaaaat ggagctggca aaactactct tattaagatg
     1921 ttaactggta ttattgcacc gtcttctgga gaaatatctg ttatgggtta ttaccctaac
     1981 aaattagaaa ataattttaa aaaacaatat gctgttgtta tgggccaaaa aagtcaacta
     2041 ttctttgaat tgacaccagc agatacgttt cttttattta aagaattata tgatatatct
     2101 aatgaagaat ataaaaaaaa cttggattac tttatcgaat tatttgaggt atctgactat
     2161 ttaaatgttc aagttaggac gctttcttta ggagaaagaa tgaaaatgga attaatagta
     2221 gcccttctac ataatccaaa aatattattt ttggatgaac caactatagg cttagatgct
     2281 atagcccaaa aacaaattag gtatttccta aaagaagtaa atgaaacaaa aggaactact
     2341 attatactta cttctcatta catggaagat attaagagtc tctgtagtag aagtattgtt
     2401 gtagaaaata gccgtaaaat atatgatggt tctacagaga aactatttaa tcattttcaa
     2461 aaatataaaa aaataacagt tagtttttcc aatgaaacta acattgtatt tccaaaagat
     2521 tgtaaagttg ttgaacaaac aaatcataag acatcagtaa ttgtgctaaa agaaagagga
     2581 aatgaaacga ttaaatggat ttttgataaa tatgatgtta aagatgtaat gattgaagaa
     2641 gaagatatag gtagtattgt agagagaata tataagaaag gaagtgatga gtattatgag
     2701 caactataat gaagcaagtt caaattattc aatgttttat aaatttaaac aaagtattag
     2761 aaggtattat gaagtagcca aaattaactt taaagtgcaa cttatctgga gatttgatgt
     2821 ggtggttaat atggtgttta cgattgtaaa aattttattt gcttatattt tatggaaagc
     2881 aatttttggt gaaaataaaa tagtctctgg ttttacattt aactctatgt tgtcttacta
     2941 tataataagc tcatttttag caggtattga tatgtcaaag agtaccagtg aagagatatg
     3001 taatcgtgta aaattgggca cattttcaaa gtacatgatt attccaatta atgttcaaaa
     3061 ttattttatt gctaaaaatt ttggaatgac atgtttttat tttgtattta atatgatttc
     3121 tgcatttgta tggattttta tatttaaaat agattttgcc tttacatcaa atccagttca
     3181 aatattaatt tcaattttga tgatttttct agggtttatt tttatgatga aactccactt
     3241 ctttattggg attttgtcat ttaaatttct tgagattagt ttttttagaa tgataattga
     3301 aaatattgtt gaatttatta caggaacaat gataccactt gtactcttac cagaaactat
     3361 agtaacagtt atgaaggtat ttccatttta ttatacaaca tatttgccat ctatgttact
     3421 aataggtaga aacagcaatg aggcaataca aggattaatt attctagcat tatgggtttt
     3481 atttttttct gtagtaaata aagttatgta taaaagaatg agaacattat ttgatggggt
     3541 gggtatatga tgaaaaaaaa tttgaaaata atatttatgt tattaaaaat gaaattatca
     3601 aagatgatgg tatttagatt tgattttttt ggagcatttt ttgtagatag ttcattattt
     3661 atattacaac ttctaatgtt taatagtata tattctcatg tagactcaat agggggatgg
     3721 caacaaggag agatgttaat atttatagga actttctcat taataaatgc gataaatatg
     3781 acgattttct tttttggtat ttatgatatt ccacgaaaaa tccaagaagg agaattagac
     3841 tactatatta caaaaccatt agacccacta tttaggttaa catttgaaaa tattaatatt
     3901 ggttcatcac cattaattct agcaagtata cttattgttt tatatggaat atctaagtta
     3961 aactcagaga taactttagt tatgataata atatatattt tcatggtgtt attgatgaca
     4021 ttactttttt atgatgtatg tatcatactt agaacagttt cattttttgt tatatcatct
     4081 tctgcgataa tgagattaga agataattta ttgccaatga atatgaaaat tcctggtgta
     4141 atatatgaag gtgtttttaa agtattattc tatttaatct taccatatgg aattatgtcg
     4201 acaattccga ctcaaatttt ggctgggaca ataacaatta atggattaat atactcaatt
     4261 ttaattgtat ttttgtttac attgtttatg tggagatttt ggaagttagg tatgagacat
     4321 tataaaagtg caagtagtta ataaattagt aaaaatagac aatgtataac tatatataaa
     4381 tacgtttcat atgacactaa actatgtgaa caagtatttg cgctaaaggc actgaaagct
     4441 ataaaatgat taaagtaagg atttaaaagt ttctttagta gaaatataat tgtgtaaaaa
     4501 agcaagatta attaaaacta tatccaaata attagatata attcttatgc agaaaggagg
     4561 tctgtaaatg tctaattgtt tggaatatat tttaaaaata attaatgtac agaaaaataa
     4621 acaaaatgag tgatgtatat tattcagaaa atcatgaaca agataatctt tatgagcaag
     4681 ataatttact ggcacaaaga tttaatttat ttactctatt gaagtttgca tttcctacta
     4741 tttttatgat gatttttatg gggttgtata caatcgtaga tactatattt gtatcaagat
     4801 ttattaatac aaatgctcta tcagcaatta atattgtatg tccaattatt aatcttatag
     4861 ttggaattgg aactatgctt gccactggag gaagtgctat tgtagcacgt aagatgggaa
     4921 atggtgatat tgagggagca aataaagctt tttctttaat cgtattatca ggagttgtaa
     4981 ttggggtaat aattactggt ataggtattg tatttatgga ttatatcatt atatggttag
     5041 gagcaagtga agttttaatt aaatattgtc gttcatatct ttttattatc ttggtttttg
     5101 cttctgcaaa tatactacaa gtattatttc aatgtttatt tgtaacagct ggaaagcctc
     5161 aatttggatt atggctagtg ttaggtgctg ggtgtgctaa tataattctt gactatgtgt
     5221 tcattgttct gttacaaatg ggtattagtg gagcagcact tgcaactgga gtaggatacc
     5281 taattcctac cgtttcaggt atggttttct tttcaaaaaa taaagagtta cttaagtttt
     5341 gtatacccag actagataaa agaatattat ttgaagcttg ttttaatggt tcatctgaaa
     5401 tggtaggtca actatcaaca gcagttacta cttttttatt taatgctatg atgatgaaat
     5461 taattggtga ggatggagtt gcagctatta caataattat ttattctcaa tttcttttaa
     5521 cttcgcttta tataggtttc tcaatgggtg ttgcaccaat aataagttat aactatggaa
     5581 gtgaaaataa tacacagtta aatcatgttt ttaagatttg tatattattt atttcagtgg
     5641 catctttatt ggtatttatc ttttctatat catgttcacc aatcattgta gagatttttt
     5701 ctcaaagagg aagccgagtt tatgaaattg caaggtctgg attttatata ttttctttta
     5761 gttttatttt ttgtgggtat aatattttta cttcgtctat gtttacagca ttgtcaaatg
     5821 gcaaggtctc tgcaatatta tcttttctac gtacatttgg atttataact ataggtttat
     5881 tgatactgcc aaatatatta aatataaatg ggatatggtt agcagtacca agtgctgaga
     5941 ttttaacttt atttttaacc attttttgta tatgtaagta taagaataga tataaatata
     6001 tgtagatatt aaataaatag tttaggtaga tattttatgt tttaataagt aattcaatta
     6061 gtggtagata tatactattg aaaattaaag ttataattga tataaagaat taataaaaat
     6121 aacatataaa agtttaaaag ggagttgaac ttaaagtttg attagaaaaa gtcaatttta
     6181 agacaactcc ttttatttta ctattgtatt aatctgtatc tgtatctgta agtgtattag
     6241 tctaatatta taaactttgg atgaaattaa ttttctatag gttttaatgt atcatacact
     6301 ttattttaaa gaaacttatc ttaattttca tagtgattga atttgtctat tagagatgtt
     6361 actattagta atgtaaaagc aagagtagtg aatataaaaa aacaagatat tgatactgca
     6421 agattagaat ataataaatt tttatggaga gatttcaata tggatacata ttttaataaa
     6481 aaaatattac ttgttgatga tgaaaaagat atagtagatt taattgaaga agtactcata
     6541 aatgatggtt ttaaaaacat tattaaagct tataatggtt tagatgctat ttcgctgtgt
     6601 aagatagcat gtccagatgt tgtcattctt gatataatgt taccagatat agatggtata
     6661 gaagtgtgta aaaaaattcg agagttttca tactgttcta ttcttttttt atcatcaaaa
     6721 aatgatgata ttgacaaaat actaggtctt agtagtgggg gagatgacta tataacaaag
     6781 ccattcagtc caagagaaat tgcttttcga gttaaagcgc aattacgtag acaacagtat
     6841 caaagcattg taccatcaga tagtgcagtt attaaaatag gagatattac tattgatata
     6901 gaaggaaacc gtgtatataa agatagaaat gagattgagt taactggaag ggagtatcat
     6961 cttttaagct acatggctca aaatgtaaat aaaattatag gaaaagaaag actctatgaa
     7021 caagtatggg gagtatatag tagtatttgt gacaatacaa ttatggtgca tatacgacat
     7081 ataagagaaa aaattgaaga taacccctca aatcctaaaa tacttataac ggttaaagga
     7141 ctaggatata aacttgtgaa tagaatcgat tagaatagat tttaatatta atgaagatgt
     7201 ctcaagttga atttttagtt aatgaggcat tcttttatgt gtgaagtcaa atgtattttc
     7261 gttatctcaa caatgaagaa gaggtctatc tctattttga gatagcctca ttaagtttat
     7321 atcctggtaa tgtactttat gttctaataa aattttttta gtttatttgc agtgaaacat
     7381 taactagaac attaaaattt gcattgaaat atcaattaaa taatattgta tattgtgtat
     7441 aaacttatac taattgtttt tctatattaa acactttatc aatccaacct ttataaaatt
     7501 tttcttcatg ggaaactagg attatacttc ctctaaactg tttcaaagca ttttgtaaag
     7561 catcctttgt ctctgcatca aagtgattgg ttggttcatc cagaataaga aaattacaag
     7621 gagataacat catacaacat aatttaacct tagattgttc accaccactt aaagtagata
     7681 cactccttga aacatgctct tccttcacac cacatctggc tagatgacgt ctaatctctt
     7741 ttgtatttaa ttttggatat ttatctgcta ctatttgaag tggggtttta tcaggatttt
     7801 cccattttaa atcttgctca taataaccaa ttttaacttg ttctgaaaac tggaaatctc
     7861 cagatatacg aggaatatct ttgactaatg tctttaacaa ggtagattta ccgattccat
     7921 taaaaccagt tataactatt ttttgacctc catcaacaga aaaatttaat tttggaagta
     7981 aagaatagta gtatccaact tcaagatttg ttatagtaag agcattttga gccgatattt
     8041 caatttctga aaattgaata tttggttttc ctgtaaaact aggaggagct attctttcta
     8101 ttttatccaa ttgtttttgt cttcctcttg caatttttga atttactcca gctttatttt
     8161 tacgtataaa tgtctcttct ttttctattt tcttttgttg tgcttgatac cttctaatat
     8221 aatcttctct taaatgtgat ttttgtttaa ggaactctga atattttcca tgatattttt
     8281 taatcatacc aaattctata tcaagaatac ctgtagaaat tttttctaaa aaatcaaaat
     8341 cgtgggaaac tacaataaat gccccattaa atgtatttaa ataatttgag agccattcaa
     8401 catgttcttt atctaaaaag tttgttggtt catctaataa tagaacatca tggttagaaa
     8461 gtagcaattt tgctaaaatt acctttgccc tttgcccacc actaagctct tcaacaacac
     8521 gattcattcc tattgaatct aatccaagac catttgctat tttatttatt tcattatcta
     8581 tagaatagaa attacttgct attagttgtt cctgataatc tgatgctttg attagatatt
     8641 gctcattttc agatatagca cttttttgat agagtaaatt catttccttt tctattttat
     8701 aaagttcttc aaatgcagtg tgtaaataga gtgatattgt tgtatctcta ttgatttctg
     8761 catattggtc taaatggcct atattgatat taggttgcca cttgatttct cctgaatctg
     8821 gaacaacttc tcccaataaa attttaatta atgtactttt acctgtacca ttttggccaa
     8881 ctacacctat atgttcacct ttgaataaat caaagttagc tttctcatac agtgatttat
     8941 ccataaaaca ttgtgataaa ttagttactt ttaataaact cataattcaa actccttttc
     9001 ttttattaaa aaatttatgg aaataaaaaa agcagaggaa tggcattgta aaatgccgac
     9061 ctctgctttt ggaaaaacaa aaggtcatag acaagcattg ttgtctatga ccagaaaatc
     9121 tatactatct atcggcaaat aggtaatttt aaataagcat agccaaaaaa ctatactaaa
     9181 cctatatttg ccgatgaaga tagaaagctt taagctttca gattaacctc tacacaacgc
     9241 ttcatcgtat acatttgtgt agagttaagt tttctaacaa gtttaactaa ttctatataa
     9301 atcataatta ttcacatcct aattttagta ttaattctag tttatctata tttaaattgt
     9361 acttttgtac tttgtggatt atcttattta tttaaaatat gttttattaa ttatagctta
     9421 agttatgtta tcataatact tggttatagt caataagaaa gttatatttg ctatatatta
     9481 tattaattta gtaaatctat cattacaaag ttatttgaag actcgctacc aataaaattt
     9541 tatagtctat gggaaaatta agattatgag ttttatactt ttgatatagg gatactattt
     9601 tcataaatat tacaacatgt agaagtatat gtctagtatt gttgaatttt caatgatata
     9661 ttaaggttct aatatattat acaaaataaa aacattaatt caacttaaat aattttaagt
     9721 attatatgtg ttttgattaa agaaaactaa aattttaagg aaaaataaca tatttgtatt
     9781 aaattttatt gaaatgatta aatttattta cttaatgtaa attattagct aaaattgtat
     9841 taaataatta aggaggtgag tagctctgga tgaaatagat ttaaaaataa ttaattctct
     9901 aaaaaagaat agtagggctt caacctctga aataagcaga caaattaatt tatcagtacc
     9961 agcagtctca gagcgtataa ggaaaattga gcagtcaaat ttaattcaaa agtacactat
    10021 tcaaattaat cgggaaaaaa caaaatataa gcttttggct tttatattta tcaatactga
    10081 tttatctaaa agtatgaaag aatttaagaa aactatattg gaatatgata ctgtattaga
    10141 atgtcatcat atttcaggag agtatgatta cttaataaaa gtattagcag aagatacaag
    10201 taatcttgaa gagtttatat cagtgacttt gaaggaaatt ggggggattc aaaaagctaa
    10261 tacaaccatt gttttatcta caattaaaga agaatttaat acaataaaag atatttttta
    10321 aataatttac tagtaaagtg ttaattatat tttaggagga aaatagaata tgaaagtttt
    10381 agttttttta gcaaaaggtt ttgaaactat ggaatttagt gtatttgtag atgttatggg
    10441 atgggctcgt aacgattatg ggcatgatat tgatgtagtg acttgtggat ttaaaaaaca
    10501 agttatgagt acatttaata tacaagtatt agttgataag acgatagaag aagtatgtgt
    10561 tgatgattat gatgccctag ctattcctgg aggatttgaa gaatttggat tttatgatga
    10621 agcatatgat tcatcttttt taaatctaat aagggagttt aattcaaaag aaaaaataat
    10681 cgcttctatt tgtgtggcag cattgccagt tggaaaaagt ggtgttttaa aaaatcgtaa
    10741 agctactaca tatcatttga aaaatggtaa aagacaaaga cagttaagtg agtttgatgt
    10801 aaatgtagtt aatgaaccaa ttgttgtaga taaaaatatt ataacctcat attgtccaga
    10861 aacagctcca catgtagctt ttaagctttt agaaatgttg acatctagag aacaaatgga
    10921 tgaagttaaa ttagcaatgg gatttaaact gtaatgctct atagactttc taagtaatca
    10981 gaaccccagt cacacatgga atctaaaatt ggaataatac tctttccaaa atcagatagc
    11041 gagtattcta cttttggagg aacaacagga taaaccactc ttataattaa accatctgat
    11101 tctaattcac gtaattgttg agttaacatc ttaggcgttg ctttaggaat taattttttt
    11161 aattcattaa acctcaatat agtgtcttta agatgccaaa gtataagtgt cttatattta
    11221 ccaccaatca atgcaagtgt tgcttctatt gggcaactgt aattatctaa acattttttc
    11281 atttttgagc tcctttattt attttaaagt atctttttag atactatatt acaaaaaagt
    11341 gcattattgt aatatagtac catagtgata aaattatagc ataggttaaa taaatcatca
    11401 acttagccta ataaaacaat agggggtaaa taaatgaatt tcgttgaact tacaaaaaaa
    11461 agatattctt gccgtaatta tcaagataga aaggttgaaa aagaaaaact tgagaaggtt
    11521 ttagatgttg caagaatagc tccaactggt ggtaatcgtc aacctcaaag attgattgtt
    11581 attcaggaaa aagaaggtat aaataaactt tctaaggctg ccaatatata tgatgctcca
    11641 ttagcaattc ttgtatgtgg agataaagac aaagtttgga caagaccttt tgatgggaga
    11701 caacttacag atattgacac aagtatagtt acagaccata tgatgttaca ggcaactgaa
    11761 cttggattgg caagtgtttg ggtgtgctat tttaatccag atataattag agaagagttt
    11821 agtttgccag ataatctaga accaataaat atattattga tgggatatga atctaagata
    11881 ccagagagcc cagagagaca tgaaaaaact cgtgttcctt taagtgaaat tgtttcttat
    11941 gaaactcttt aattataaaa ttaagttatt cataggataa atgtaataaa gtgtttatta
    12001 taaacaaaat tcaaaataaa taatatctac atataattaa tttta
//
LOCUS       DAEPLF010000096        12026 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEPLF010000096 DAEPLF010000000
VERSION     DAEPLF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.096"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            156..749
                     /locus_tag="KR314_002222"
     CDS             156..749
                     /locus_tag="KR314_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HBH0328604.1"
                     /translation="MRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYY
                     NLRTITWNDRVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNY
                     PYQIFEFTSSRDCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSIGPMG
                     ATGPTGATGNSSQPIANFLVNAPSPQTLNNGNAITGW"
     gene            918..1109
                     /locus_tag="KR314_002223"
     CDS             918..1109
                     /locus_tag="KR314_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HBH0328605.1"
                     /translation="MAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQ
                     SAGTAATLTIFRIADTVMT"
     gene            complement(1344..2942)
                     /locus_tag="KR314_002224"
     CDS             complement(1344..2942)
                     /locus_tag="KR314_002224"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBH0328606.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFDVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            3820..6462
                     /gene="adhE"
                     /locus_tag="KR314_002225"
                     /gene_synonym="adhC"
     CDS             3820..6462
                     /gene="adhE"
                     /locus_tag="KR314_002225"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBH0328607.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPDRVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGNTDQEKVDNLIKAIDELKARVGLPKTIKEAGVEESKFLETLDVMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(6822..7163)
                     /locus_tag="KR314_002226"
     CDS             complement(6822..7163)
                     /locus_tag="KR314_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328608.1"
                     /translation="MLLILDNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRINKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(7190..7861)
                     /locus_tag="KR314_002227"
     CDS             complement(7190..7861)
                     /locus_tag="KR314_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328609.1"
                     /translation="MPILEILNLGKIYGKKETAVHALKDANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSSGTIKVDGKDICCLSDKKLAKYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKVDEEYIEDIINLLGLSERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(7873..10458)
                     /locus_tag="KR314_002228"
     CDS             complement(7873..10458)
                     /locus_tag="KR314_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0328610.1"
                     /translation="MKDNLKIALRYIISYKARSLAIALSIILATSLIVGIGTLSRSAQ
                     QAEVDKLKRETGSDHVYFKDINKNQLEYIKSRKDIKNLGITSHYGYTDPNERLAINLE
                     YANKNYLTNQSKLIKGHLPKASNEVVVEKWILNSLGLKPEINQNITFKLYQKEKPETF
                     KVVGILEDRPIEKNKGTCEIFLNLNESKLDKFSYAYIEFNNGIDINTSIDNIVKNTML
                     DENSVGKNKMLIESTRENGTLDNSSKYTAITMSLFSGIVIYSIYVISIYQRIQEYGIL
                     RAIGSTNFKIFKFMFYELFILALIAIPIGICTGIGGAQIFNRTAGNIQFEGNVTVTPF
                     VIPDKIILLSIGSIILTILIISFFTYLKIRRISPIDSIRKTFGTDKNINKVNSLISKL
                     TLNISVTKSISAKNIFRNKKGFIIIILSMSLGGIMIIKENYKYSFSDIQNKNGQEETY
                     MNADFILTNFFLKLNQSNKADSFKDIKGLNDNQIDKIKNINGIDKVKTASILDTRIEV
                     DKLNGLDYYNIINSTPYYKDFPLFIKNKNTGKYTMKQKLRGYNDEMINSLEKYLVSGS
                     INLERMKKENLAILYVPQVSKTNKYKLSYTPGTETPAVDIKVGDTIKVKYPKGEIDQD
                     LYIKLKDNYEYLEYEFKVGAIVSYPFADNYLYSGDQGIDVITSNDYLKKLTGVDKYNV
                     VYVDINKNANVKKINTLLGEIGSESPGTTTANMLMEKENFDKMTARALTYAYGIVAVM
                     FIISVFNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGILSSVITIVV
                     GLIIQFRMYYTYNFVSYGLGFSIDYKIYILVVLANIIVGILATYIPLRKINKISIVEA
                     INITE"
     gene            10666..11601
                     /locus_tag="KR314_002229"
     CDS             10666..11601
                     /locus_tag="KR314_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH0328611.1"
                     /translation="MENNFIFPIVILTLFIFSNLIMNKYRFYKELDILKRGILTFFVS
                     CFVWILFMEGIEYIQLSLGYNFLLEGVLNIILFLILIILSFYIGFKFIRLLDNNNKQE
                     YKLQKLDSKINHKDNIIVKLRSQKHDYLKHLQVVYSLLNTGLAEDAKEYITNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKNISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSAIQKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFLDGFSTKEGQDRGF
                     GLYLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            11620..>12026
                     /locus_tag="KR314_002230"
     CDS             11620..>12026
                     /locus_tag="KR314_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBH0328612.1"
                     /translation="MIKVLICDDDKIVRDDIANIVKESGYVDYVKKVKDGIEAIEFIK
                     SEKIDLLIIDIDMPYKNGIDSAKEILSIDKDIHIVFVTGFADYSLESFKVHPIDFIVK
                     PFKKEKILDSINIAIDHINSHKIAKNNFIEDTL"
BASE COUNT         4338 a         1597 c         1744 g         4347 t
ORIGIN      
        1 gattataaat gagaaaaatt atactttatt taaatgatga tacttttata tctaaaaaat
       61 atccagataa aaactttagt aatttagatt attgcttaat aggaagtaaa tgttcaaata
      121 gttttgtaaa agaaaagttg attacttttt tttaagtgag aataccagat atattaaaag
      181 acaaaagtat attaaaagca gagttattta ttcatattga ttcaaataag aatcatattt
      241 ttaaagaaaa agtagatatt gaaattaaaa gaataagtga atattataat ttacgaacta
      301 taacatggaa tgatagagtg tctatggaaa atatcagggg atatttacca attgggataa
      361 gtgatacatc caactatatt tgtttaaata ttacgggaac tataaaagca tgggcaatga
      421 ataaatatcc taattatggg ttagctttat ctttaaatta cccttatcag atttttgaat
      481 ttacatctag tagggattgt aacaaaccgt atatacttgt aacatttgaa gatagaatta
      541 tagataattg ttatcctaaa tgtgagtgtc ttccaattag aattacaggt ccaatgggac
      601 caagaggagc gacaggaagt ataggaccaa tgggagcaac aggtccaaca ggagcaacag
      661 gcaattcctc tcagccaatt gctaacttcc tcgtaaatgc accatctcca caaacactaa
      721 ataatggaaa tgctataaca ggttggtaaa caataatagg aaatagttca agtataacag
      781 tagatgcaaa tggtacgttt acagtacaag aaaatggtgt gtattatata tcagtttcag
      841 tagcattaca accaggttca tcaagtataa atcaatattc ttttgctatc ctattcccaa
      901 ttttaggagg aaaagatttg gcagggctta ctactgagcc aggaggcgga ggagtacttt
      961 ctggatattt tgctggtttt ttatttgggg ggactacttt tacaataaat aatttttcat
     1021 ctacaacagt agggatacga aatgggcaat cagcaggaac tgcggctact ttgacgatat
     1081 ttagaatagc tgatactgtt atgacttaaa acgtgtctaa aataatctta aaaactattt
     1141 aggttttatt taaatgacaa aagtattttt atatattgag ttttacctat tttagaatga
     1201 ataaaataac aataataata aaatatattc ataaaaattt taaatttatg gatttttatt
     1261 taactttatt atcaatatat gtataataaa aaactgtctc aaatatagat ttgagacagt
     1321 tttcgttatt taaaaatttt atattattta aaatttttga ttgcagtagt taaattaggg
     1381 actaattgtt tttttcttga tacaacacct ggtgcaaatg taccttgaac accttcaaca
     1441 tcaaatgcgt tagcaattaa gctatcttct gctttataga ttaaataaga accttctttg
     1501 attatatctg taaccgcaag aattagtttg tcataatcag tcgaatttat ataagataaa
     1561 aactcatctt ttttagcaaa tatagagtct atgtctaagg taaatacttg tccaatacca
     1621 actctatgtc cactcatatt aaattcttta aaatccatat ttactatttc ttctatagta
     1681 tattcatcta aagaagtacc gcatttaaac atatccatag cgtatttttc catgtctact
     1741 tttgctattt tacttaattc ttcacaagct ttcttatcca tatcagttgt tgttggagac
     1801 ttaaataata atgtatctga taatatagca gataaaagaa gcccagctat ttcataaggt
     1861 atctcaacat tgttttcttt gtacatttga taaattatag tactattgca tccaacaggc
     1921 ataactctaa atgacatagg aacatcagta gaaataccac caagtttatg atggtcaatt
     1981 atttcaacta tgtttgcttg ttcaattcca tcagcacttt gagcatattc gttatggtca
     2041 actaaaacaa cattcttttt agatgggttt aatagatgac cttttgaaac taaacctaaa
     2101 aacttattat catcatctaa tattggataa gatctgaagt ttgtttctag cataatctct
     2161 tttatttcat ccatataatc atcttctttg aatgtaatta aattttctgt agcttctata
     2221 gagtctaaga aactacattg atttattaca ttagaagttt tataattgtc actttcaaca
     2281 gaaacaacag acactccatt tatcttagca agagctagca aatcttttga aatctctgta
     2341 tgattagtaa gtattaaaag tttaactttt gtatcaatag catattcaat actatcatat
     2401 ctatctccaa caatagctat gtctccttct tttaaagtct ctattagagt ctccattcca
     2461 aatgataaag tgactactct accttttata tcttcaccac agtccacaag aatagtacca
     2521 ttaaggtttt tacaaatgtt tgatagtgat gtgtgtatag ttgtaaagtg ttgatgaagt
     2581 agatttttag ctatttcttt catagtaatg ataccagcaa aagttccatc atcataagtt
     2641 acaggtagtg ttttaacatt tttttcatcc ataaggttat aagcctctaa tatagatgaa
     2701 gttggtgtta aaggtactac tttatcataa tttaaatctc taacttgtat tctaacatta
     2761 tctaaaattt caggtgcatc taccttaaag taatccaagg catatttagc ttcttttctg
     2821 atatctccta aagcataagc tgttgctttt attcctagtt tgttctttaa atgtgctaat
     2881 gaaattgcag aacatattga atctgtatct ggatttttat gtccgaaaac taataaactc
     2941 attttaatac tcctttcaaa acttgaatat aataataatt gataaaaata ttatatcaaa
     3001 aggtagtgag tatttgttgt aaaaagaaaa aaatttagtg aaaaaatatt atataaaatt
     3061 tatttcttac ataaatacta caaatttaat ataaaaatta ttattatata tatttttacc
     3121 caaccaaaca tagaatcaaa tttactttta tttaaaataa attgattaaa tctacctggg
     3181 aaaaaattaa ttaatatgtt aataaattaa aaatttataa atattttata ctgtaaaaaa
     3241 ttttgtatat atattatcat taaaaaatta acggtcttaa tataaaaatt atttatctat
     3301 atgaaaagca taattattac atattgactt aaaaataact tatagagatt agaataattt
     3361 tagtttgtat attttttaaa accatacagt tgttataaaa agaaaaattg cacacaagtc
     3421 aaaaataagt gttattttat aaaaatacga caaaaatttt atttactact tatacaaata
     3481 acatgcaaat aacatataaa atttaataag taaaaaaaat gaaagatata aattatcatt
     3541 ttagtatttt atttagttga agtaaattat tcaaatgaaa tatatctcat ttagataaaa
     3601 aattttagta aaagtaaaaa aaataatata tttgttaaaa aataaattga caaaatattc
     3661 agaaaaaaac ttcttgacct aagagagtaa aataaataaa atctaaggta tagagatgaa
     3721 atctataaaa ttattttaag tataagttaa aaatatagat ggtgcattta agatttataa
     3781 aaattagaga tgcataatta ttttagggag gtttttagta tggagaaaaa agaaaaggta
     3841 gtagaaaaat caaatgttga agtatgtagt cctgaaattg tcaacagtgt tgaaacatta
     3901 agaatgagat tagaagaaat tagattggca caaaaagaat ttgcaacatt tacgcaagaa
     3961 caggtagata agattttctt agctgcttca actgcagcta atcaacaaag aatacctcta
     4021 gcaaaaatgg cagtggaaga gactggtatg ggtattgttg aagataaggt tataaaaaac
     4081 cattttgcct cagaatatat ctataatgct tataaagata ctaaaacttg tggtgtcata
     4141 gaaaaagatg aagcatttgg ttttacaaga attgctgagc cagtaggtgt tttatcagca
     4201 gttataccaa caactaatcc tacatcaact gcaatattca aatccctaat agcactaaaa
     4261 actagaaatg gtataatttt ctctcctcat ccaagagcta aaaactgtac tatagaagca
     4321 gctagagttg tacatgatgc ggcagttaag gcaggagcac caaaaggact tataggttgg
     4381 gtagatgttc cttcgattga attaactaat gttgtcatgg cagaggcaga ccttatatta
     4441 gctactggtg gacctggaat ggttaaatct gcatactctt caggtaaacc agctgttgga
     4501 gttgggcctg gaaatgtacc tgcaataata gatgagagtg cagatataaa aatggctgta
     4561 agttcaatct tagtatcaaa atcattcgat aatggtatga tatgtgcttc cgagcaagct
     4621 gtaatagtac cagaaaaaat atatgaagaa gttaaaaaag aatttaaata ccgtggagca
     4681 catttcctaa ataaagaaga aactgaaaaa gtaggaaaag tagtaataat tgatggttca
     4741 ttaaatgcca gaatagttgg acaaccagca catgtaatag ccaaaatggc ggatgtagaa
     4801 gttccaaaaa cagctagaat aataattggt gaagtagaat ctgttgaatt aaatgaacct
     4861 tttgcacatg aaaaactttc tcctgtactt gcaatgtaca aatctaaaag ttttgaagat
     4921 gcagttgcta aagctgaaaa attagttgca gatggtggat atggccatac atcttcgtta
     4981 tatgcagact ctataaatca tccagataga gtagagaagt ttgtaaatgc tatgaaaact
     5041 tgtagagtat tagttaatac tccatcgtca caaggtggta taggtgactt atataacttt
     5101 aaattagcac catctcttac tcttggttgt ggttcttggg gtggaaactc agtatctgaa
     5161 aacgtgggag tgaaacattt acttaatatc aagacagtag ctgaaaggag agagaatatg
     5221 ctttggttta gagcaccaga aaaggtttat ttcaaaaaag gttgtttagg tgtagcagca
     5281 agagaattta aagatgttat ggataaaaag aaagccttta tagtaacaga ttctttcctt
     5341 tacaataatg gatacactaa gaagctaact gatttgttgg atgagatggg aattaagcat
     5401 actacattct ttgatgttgc tccagaccca acattagctt gtgcaagaga aggagctaag
     5461 gctatggctg atttccaacc agatttaata atagctgtag gtggaggtag cgcaatggat
     5521 gctggtaaga taatgtgggt aatgtatgag catccagagg tagacttcca agatttggct
     5581 atgagattta tggatataag aaaaagagta tatgtatttc ctaagatggg tgaaaaagcg
     5641 tattttgctg ctatcccaac ttcagcagga actggttcag aagtaactcc atttgcagtt
     5701 ataactgacc aagattcagg tgtaaagtac ccattagcag attatgaatt aatgccaaac
     5761 atggctataa ttgatgctga tatgatgatg gagatgccac caagattaac agcagcatca
     5821 ggtgttgatg ctttaacaca tgcattagaa gcatatgtat ctatgttaag aactgaacca
     5881 gctgacggtc tagcactaca agcaggtaaa ataatatttg aatatctacc tcgtgcatac
     5941 aaaaatggaa agaatgataa agaagcaaga gaaaaaatgg caatggcatc tactatggca
     6001 ggaatgtctt ttgcaaatgc cttcttagga atatgtcact ctttagcaca taaattaggt
     6061 gcattccacc atgttcaaca cggtgttgca aatgcattat taataaatga agtaattaag
     6121 ttcaattgtg cagaagctcc aaataagatg ggtgcattct cacaatacag atatcctgat
     6181 tgtatacaaa gatatgctga atttgcttct tttgcaggaa tcaagggaaa tacagaccaa
     6241 gaaaaagtag ataaccttat aaaagcaata gatgaattaa aggcaagagt aggacttcct
     6301 aaaactataa aagaggctgg agtagaagaa tctaaattct tagaaacatt ggatgttatg
     6361 gttgaacaag cttttgatga ccaatgtaca ggtgctaatc ctagataccc attgatgagt
     6421 gaattaaaag agatatatct aaaagtttat tatggtaaat aatattcatt gtacaacata
     6481 ggttctattg ggtttgtata taaattatta gatttgaata taaatagagt tttattagag
     6541 aaacgtagaa gtaactataa ataatagatt ataacttaaa ttctgaatgg aaatgataaa
     6601 aagtcagtaa tttaatggta atttcgaata tgtgtattta atcaattaac tttaaaatta
     6661 gtcggaaaat ttttttatta catataattt taaagcataa tatataaaaa actttatgaa
     6721 aaaattataa aaatattcag aaaattctat tgactaattt atttatcatg atataataaa
     6781 tttagaaaag aaaaaaattg gggcctatta aggccccctt actatttttt gatagattta
     6841 tttaaaatta ctttctcatt ttgatttttt atatttatta tagagtcagt ataatccact
     6901 tcaaagttgg caaaagcaga gaataaatct atatctctta acaaatgaat tgtcatacat
     6961 tgattgttct tatttatcct tagaatcaat gctcttacaa atggaaattg tattctagaa
     7021 ttaaataagt tagaaccatc agtatctata tctaagctac gataattaat atctataaat
     7081 atatctacat catcattttc ttctcttatt attctgtagt caaaatctaa gtttaaattt
     7141 aaattatcta aaatcaataa catttaaatc ctcttttcta taaatagttt tagagtgtta
     7201 atttaagctc tccatctatt atagatataa ttctatctgc ttttctagca atattttcat
     7261 tatgtgttat tattataagt gtttggttat atttttttat agaacttttt agtaaatcca
     7321 tgacttcttc cgtagtttta ctatccaaat ttcctgttgg ttcatctgct aggattatac
     7381 ttggttttgc agataaagcg cgtgcaatag caaccctttg ttgttgacca ccagataatt
     7441 gatttggaag atgatttttc ctctcactaa gacccaataa atttataatg tcttcgatgt
     7501 attcttcatc aactttttta ttgtctaact ttagtggtaa ttctatattt tcttttacat
     7561 ttaatactgg aattaggtta tactgttgaa aaacaaagcc tactttttgt cttctatatt
     7621 ttgctaattt tttatctgaa aggcaacata tatctttacc atcgactttt atagttccac
     7681 tacttggtct ctcaagacct cctactaaat gtagaaatgt gctttttcca cttccacttg
     7741 ggccaattat cgcaacaaat tcacccttat ttatttttaa atttgcatcc tttagtgcat
     7801 gtacagctgt ttcctttttt ccataaatct tacctaagtt aagaatctct aatataggca
     7861 taattaatcc tcctattctg taatgtttat agcttcaact atactaattt tatttatttt
     7921 ccttaatgga atatatgtag ctagaatacc tacaattatg tttgctaaaa caactaggat
     7981 atatattttg taatctattg aaaatcccaa tccatatgat acaaaattat acgtgtaata
     8041 cattctaaac tgtattataa gccctaccac aatggtaatt acacttgaga gtattccata
     8101 taatattccc tcatagagta tcatattttt aaatcctctc tcagagatac caatagctct
     8161 taacattcca aattcgctag ttcttgaagt taagttataa cttatgttat ttattatatt
     8221 aaacacacta attataaaca ttactgctac tattccataa gcataagtca gagccctagc
     8281 tgtcatctta tcaaagtttt ctttttccat tagcatatta gctgttgtag ttccaggaga
     8341 ttcacttcct atttcaccta agagtgtatt tatctttttt acatttgcat ttttattaat
     8401 atctacataa actacattat atttatctac accagttagt ttttttagat agtcgttact
     8461 tgttattaca tctataccct gatctccaga atataggtaa ttatctgcaa aaggataact
     8521 tacaatagct ccaactttaa actcatattc tagatattca taattatctt ttagttttat
     8581 ataaagatct tgatctatct caccttttgg atatttgacc tttatagtat ctcctacttt
     8641 tatatctaca gcaggcgttt ctgtaccagg agtataactt agcttgtatt tatttgtttt
     8701 agaaacttgt ggtacgtata atatagcgag gttttctttt ttcattcttt ccagatttat
     8761 actcccactc actaagtatt tttctaatga atttatcatc tcatcattgt aacctcttaa
     8821 cttttgtttc attgtatatt ttccagtatt cttatttttt atgaatagag gaaaatcctt
     8881 ataataaggc gttgaattaa taatattgta gtaatctaat ccatttaatt tatctacttc
     8941 tattcttgta tctaagatgc tggcagtttt gactttgtca attccattta tattttttat
     9001 tttatctatc tgattatcat taagtccttt tatatccttg aaagaatcag ctttattaga
     9061 ttgatttagt tttaagaaaa aatttgttag aataaaatct gcattcatat atgtttcttc
     9121 ttgaccattt ttattttgta tatctgaaaa agaatattta taattctcct ttatgatcat
     9181 tatacctcct agactcatgg ataatataat tataatgaag ccctttttat tcctaaagat
     9241 atttttagca gatatagatt ttgttactga tatatttaaa gttaattttg atatcaaaga
     9301 attgacctta tttatatttt tatcagttcc aaaggttttt cttatagagt caataggaga
     9361 aattcttctt atctttaaat atgtaaagaa acttataatt agtattgtca gtattatact
     9421 tccaattgat aaaagtatta ttttatctgg aataacaaaa ggggtaactg ttacattacc
     9481 ttcaaattga atatttcctg cagtcctatt aaatatttgt gcaccaccaa tacctgtgca
     9541 tataccgata ggtattgcta tcaaagcaag tatgaaaagt tcataaaaca taaacttgaa
     9601 aatcttgaag ttagtggatc caatagctct taagatacca tattcctgta ttctttgata
     9661 tatcgatata acatagatac tatatattac aataccagaa aataggctca tagtaattgc
     9721 agtgtattta cttgagttat ctaaagtacc attttcccta gtagattcta taagcatttt
     9781 gttttttcca acactattct catccaacat agtatttttt actatattat ctatgcttgt
     9841 atttatatca atgccattat taaattcaat ataagcataa gaaaatttat ccaattttga
     9901 ttcattgaga tttaaaaaaa tttcacatgt ccctttattt ttctcaatag gtctatcttc
     9961 aagtattcct acaactttaa atgtctctgg tttttctttt tgatataatt taaaagtaat
    10021 gttctgattt atttctggtt ttaaacccag actatttaaa atccatttct ctacgaccac
    10081 ctcattacta gcttttggaa ggtgaccttt tataagtttg gattgattag tcaagtaatt
    10141 tttatttgca tattctaggt ttatagctaa tctttcatta ggatctgtat agccatagtg
    10201 agatgttata cctaaatttt taatatcttt tctagacttt atgtattcta attgattttt
    10261 atttatatct ttaaagtata catgatcact tccagtttct ctttttaatt tatctacctc
    10321 tgcttgttga gcacttcttg agagggtacc aataccaaca ataagtgatg tagcaagaat
    10381 tatacttaga gcaattgcta aagaccttgc tttataactt attatgtatc ttagtgctat
    10441 ttttaaatta tctttcatat ttaccacttc ctttcttagc taaatcaatt atatgtttat
    10501 ttaaaatata tatcaaagta agtatcttca agttttgagt acttaattgt acaaatttat
    10561 atgtgtattt gcaagtttaa ttaatgtaca tgtaaatttt gactttttac ataatttttc
    10621 taaaatgata taatcctcat ataaagtgta gatgggaggg gaattatgga gaataatttt
    10681 atatttccta tagtaatact aactttgttt atatttagta atcttataat gaataaatac
    10741 aggttttata aagaattaga catattaaaa agaggaatat taacgttttt tgtatcgtgt
    10801 tttgtatgga ttttattcat ggagggtatt gagtatatac aattaagttt aggatataat
    10861 tttttacttg aaggagtatt aaatattata ctttttttaa ttttaattat tttaagcttt
    10921 tatataggat ttaaatttat aagattacta gacaataaca ataaacaaga gtataagcta
    10981 caaaaattag acagtaaaat aaatcataag gacaatataa tcgtaaaatt aagaagtcaa
    11041 aaacatgact atttaaaaca tttacaagta gtatattctc tattaaatac tgggttagct
    11101 gaagatgcaa aagaatatat aacgaatata tcaaatagct ttaaatcaaa aaattcaaga
    11161 tatgggaaag tatcttattt agatgctata gcttccttaa aatatgagga atgtcttgaa
    11221 aagaacatat cttttgatgt atatatccaa gattttatgg ataatttaga gattgaacct
    11281 aatgatatga gttcagtgct attaaacata atagacaatg caatagaatc tttgtctgct
    11341 atacaaaagg aaaagaagta tataagatta catgcatatg aagatgaatt tcagtatatt
    11401 atagccataa aaaataatgg tcctaaaata cataatgtcg aaagtatatt tttggatgga
    11461 tttagcacta aagaagggca agacagaggt tttgggttat atctagttaa aaatatacta
    11521 gataagtatg attatagtat acttgtaaat agtgacgatt ttttaactga atttgtaatt
    11581 ttaataccaa aatattatta gttaggagga gagaagtctg tgataaaagt tttgatttgt
    11641 gatgatgata aaattgtaag agatgatata gcaaatatag ttaaagagtc cggatatgta
    11701 gattatgtta agaaagtcaa ggatggtata gaagcaattg aatttataaa aagtgagaaa
    11761 atagacttat tgattattga cattgatatg ccatataaaa atgggattga ttctgccaaa
    11821 gaaattcttt ctatagataa agatatacat atagtttttg taacaggttt tgctgactat
    11881 agtcttgaga gctttaaagt acatcctata gattttatag ttaagccgtt taaaaaagaa
    11941 aaaatacttg attcaattaa tatagcaata gaccacatta actctcataa aattgcaaaa
    12001 aataatttta tagaagatac tctttt
//
LOCUS       DAEPLF010000097        11945 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEPLF010000097 DAEPLF010000000
VERSION     DAEPLF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11945
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.097"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            79..780
                     /locus_tag="KR314_002231"
     CDS             79..780
                     /locus_tag="KR314_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBH0328613.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIINIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1051..1869
                     /gene="larE"
                     /locus_tag="KR314_002232"
     CDS             1051..1869
                     /gene="larE"
                     /locus_tag="KR314_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBH0328614.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNMDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKILGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLFNLGFSQFRVRM
                     HGEIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            1924..3072
                     /locus_tag="KR314_002233"
     CDS             1924..3072
                     /locus_tag="KR314_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBH0328615.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3208..4596
                     /locus_tag="KR314_002234"
     CDS             3208..4596
                     /locus_tag="KR314_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328616.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYTTLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4625..5323
                     /locus_tag="KR314_002235"
     CDS             4625..5323
                     /locus_tag="KR314_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0328617.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     ENDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            5326..6759
                     /locus_tag="KR314_002236"
     CDS             5326..6759
                     /locus_tag="KR314_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0328618.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            6781..7518
                     /gene="truA"
                     /locus_tag="KR314_002237"
     CDS             6781..7518
                     /gene="truA"
                     /locus_tag="KR314_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBH0328619.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    7629..7836
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8089..9288
                     /locus_tag="KR314_002238"
     CDS             8089..9288
                     /locus_tag="KR314_002238"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBH0328620.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            9535..10581
                     /locus_tag="KR314_002239"
     CDS             9535..10581
                     /locus_tag="KR314_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBH0328621.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DGGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDERIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPSKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            10689..11852
                     /locus_tag="KR314_002240"
     CDS             10689..11852
                     /locus_tag="KR314_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0328622.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKIIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SKNPSSTDLQEIAKEITDISKKDYEIFSMYTGDDNWYYMIQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
BASE COUNT         4886 a         1125 c         2148 g         3786 t
ORIGIN      
        1 aataatagtg gttttaaaaa tctgacaaca aaaataaaac tttcaaaaaa ttaaatctaa
       61 aaagaatagg agataaatat gagtattgaa gatttatatg attttgaatg tgttcctttg
      121 aacaatgaga ttgatattga tgttttattt gaaaagatga tagaagatgg aaaagaaaaa
      181 gaatatacac caattatagt cattgatgaa aaagtagggc tattaagaaa aaatatagat
      241 atgattacta aagagtatgg ttctcttgaa aactatagag agtattgttt aacaaaatat
      301 aatgaaattg atgtaaatca attttttgat tttaggaaga gtgagatttc agaattatta
      361 aaggagctaa ttgatttaga agatgatttt tatgattata aacattttag accaattata
      421 aatatagata tatttcatga atataacaaa gtttacatag ctaaaattcc aacaattaaa
      481 ccatatgaag tatttgcata catacctatt ggaggattta atgattgtcc tagagatgaa
      541 gaacatatag ctattgcaaa atattggtat gaaaagcatg gaatctttcc aatcgcaatt
      601 ggatgtgata cagttcaata ttctgtaaaa aatttaacta aagatggtaa aaaatttgat
      661 gacttatgta ttgaattagt attttattgt gaggatatta ttatacaagg gtatgaaacc
      721 ttaaaggctc ttaaggatgt attaaaacgc tctacaatag tgcttttttg gtgggattga
      781 tattctatca tttagatagt attaaattgt tagtcaccaa gaatccactt actttagtta
      841 tggggagtgt gtcaaactag gtttttatac acagtgtagc atagtaatat gtttaaatta
      901 caaaggttaa aaaaatactt taatattttt aatattataa ttaatagatt taaaggtaat
      961 tataatttat aaaataaata ctttatatgt tataatcatt aagaatgtaa aaaaataata
     1021 tatagtttta tagttcatat ttttccagat atggaggtaa attttgtgaa ggaaaaagaa
     1081 aaattagata aattaaaaaa aatgttactt gaattaggca gtgttgtagt tgcttattct
     1141 ggtggagtag atagtaattt tttgcttaag gtcgcaaaag atacattagg tgaaaatgtt
     1201 atagctgtga ccattcatgc aatgatgcat tcaagtaggg aaattgaaga ggctaaacaa
     1261 tatactcaaa attttggcgt taaacatatc atattaaata tagaaaactt tgatttaaaa
     1321 gaatttaaag aaaatggtgt tgatagatgt tatcattgta aaaaatatat attctctaaa
     1381 ataaaagagg tagcaaaaga acataatata aagtacatag tagatgggac aaatatggat
     1441 gatttaggag actatagacc tgggcttaag gcattgagtg aattaggagt gattagtcca
     1501 ttaaaagata gtagtttaaa aaaagaagaa attaggtcgc tttcaaaaat attaggatta
     1561 aatactttta ataaaccatc atttgcatgt cttgcaagta ggattcctta tggagttgag
     1621 ataacagatg aaaatttaag aatagtagaa aagagtgaag aatatctatt caatttaggt
     1681 ttttctcaat ttagagtgag aatgcatgga gagatagcta gaatagaagt tgggcaggaa
     1741 gaactaggta aattttttga aaataacaat tttaataaag ttgatactaa attaaaaata
     1801 tttggcttta aatatgttac actagatatg tcaggatata aaatgggaag tatgaattta
     1861 aatgtgtaga ttattttaaa aataaataag ttttatagtt aatataagga aacaggaggt
     1921 tttatgaaaa attatacatt aatatcgcca tgtttttttg gaatggaaaa aatgctggct
     1981 agagagatta ctaatttagg atatgaaata ataaaaacag aagatggaag aataacatat
     2041 aaaactgatg agtttggaat agcaaagtca aacatgtggt tgagatgtgc tgaaagagta
     2101 catcttaaaa ttgctgaatt tgaggcaaaa agctttgatg aattatttga aaatacaaaa
     2161 agaataaact ggtctagata tataccttat ggagctcaat ttcctatatc aaaagcttcc
     2221 tctataaaat caaaattata cagtacacca gatgtacaag ccatagtaaa aaaggctata
     2281 gtagaaagct taaagaaaag ttatttagag gatggtttgc ttaaagaaga taaggagaaa
     2341 taccctattt ttgtatttat acacaaagat aaagtaacca tttctataga tacaacagga
     2401 gatgctttgc ataagagagg atatagagaa aaggcaaata aagctccaat aagagagact
     2461 ttagctgctg ggcttattta tttaactccc tggaaagcag gtagagtttt agtagaccct
     2521 atgtgtggtt ctggtactat attgattgaa gctgctatga ttggtataaa tatggcacct
     2581 ggcttaaata gagagtttat atcagaaaag tggagaactc ttgataagaa aatatggtgg
     2641 gatgtaagaa aagatgcttt taataagata gataatgaat ctaaatttaa gatttatgga
     2701 tatgatatag atgaagaatc tatagatata gctagagaaa atgctgaaat agcaggtgta
     2761 gatgaatata tagagtttaa tgtaggagat gcaactcaat ttaaaagtga agatgagttt
     2821 ggatttataa tcactaatcc tccttacgga gaaagattag aagataaaga cagtgttaaa
     2881 cagttgtata aggagcttgg ttatgcattt agaaaattaa aaaattggtc gtattatttg
     2941 ataacttctt atgaagactt tgaatatgag tttgggcaaa aagcagacaa aaaaagaaaa
     3001 ttgtataatg gtatgttaaa aactaacttt ttccaatatc cgggtcctaa gcctcctaga
     3061 aataataaat aagttttaca tgatttattt gtaaaatata aaattttatt aaaataagta
     3121 aaacattatg atgccttgaa acgtctaatt attgtgagga gatttttgtg aacaaattta
     3181 aagatttata ggattaggag gagttaaatg aaagtgttaa agcttattac tgtagtagcc
     3241 ctgatgatgt tatttgttac tggatgtaat ataaaaactg aatctccaga acaattagcg
     3301 aagatacctg actatgatga tacaaaaaaa gtattgtatg atggtattga ccagttacta
     3361 aaaccagatt catctatgat attgccttct aatacaaaag aagtaggaaa aataaataag
     3421 gtagatttaa acagcgatgg aattgatgaa ttagtagtat ttgaacaaaa agaagattta
     3481 agtaataatg tgagtcaggt agggtttgta accttgagtt acaatggaga aaaatacgta
     3541 cttggagacc attttttaga aaatggcgaa tcaatagaat atgctaattt ttatgatttg
     3601 gactcagatg gatataagga agttatctta ttggttaaat ccaaagacaa aacgaatatg
     3661 catatatata aagtagtaga taatgaaata acaaaaatat atgacttgga tgcatcatgg
     3721 cttcaaaata aagaagattt taatgacatg aaagtaaaaa ttggatatat agatggagat
     3781 gacaagttag acatattgat gcttcatttg aataataaga ctaatgaaat gtttgcaagt
     3841 gttgcaaatt ttgatgggaa aatgaaaatc aaagattctg ttaagtttga aaatgtaaaa
     3901 aacttaagtg aattatatac aacattaggt aatgttgctt catctgttgg aaattcttct
     3961 gcagctataa aaggtattgt acttgatatt cctatattga aagataataa ctatattacg
     4021 cagatacttt atatgaaaga tgggaaaatt aacaaagctt ttagtgatta tgataaaact
     4081 attactaaat cttattatat acctgtagat gatattgaca aagataagat tatagaaata
     4141 cctatagttg caggcagtac aggtaataat aaaaatactt atagttctaa atcatctgca
     4201 actataagtt ggtatagatg gaatggaaaa gaaggtgctg actcaagttt aatatttaca
     4261 agtcagatat attataatta taaatataat tttaaattat ttattccaaa taatttattt
     4321 gataaaatac ttgttgaaca ggaatttgtt ggagaaaaag cagtatttaa attttactat
     4381 tttgactatt cagatcgtaa gaatttattt aatatagtag tagagagtaa aaacaaactt
     4441 gaagatagaa aaaatacagg tactcaaaac tctattgtac ttcaagagag cgatgaatat
     4501 actttcttac tagttgtaaa caatgcaaat gaaatggata agctagatat gactatagac
     4561 gcattaaaag aatatttctc attaatatat ggttaatatt atactcaatt ggaggtttta
     4621 ttttatgaag gaaaagatac ttatactaga agatgaaatt ggtattagaa gttttgttag
     4681 tataaactta aaaagagaag gctatgaaat agttgaggca ggtactggaa gagaagctat
     4741 agaaaaaatg acaacagaaa atgatattac aatagctctt ttagatgtga tgcttccaga
     4801 tataagtggt atagaagtat gtaaatttat aagggagaat tttgaccaag taggtataat
     4861 aatgcttact gctaaagctc aagaggatga taaaatagaa ggatttattt caggagcaga
     4921 tgattacatc ataaaaccat ttagtataaa agagctttta gtcagggttt ctgctcttct
     4981 tagaagagta gcaaaggatg attcaagtgt taagtcgagt gaaatagttt ctccaccatt
     5041 tatacttgat atagacaaga gaaaattatt taaaaatgga aaggaaattg agcttacacc
     5101 aactgaattt tcaatagtga aatatctaat atcaaatgct aagcaatcgt taagtagaga
     5161 ccagatatta gacgaagtat ggggaactaa ctatttatat gattttaaaa tagtagatgt
     5221 aaatataaga aggataagaa ataaaataga agatgaccca tcaaagccaa aatatataca
     5281 aactatttgg ggttatggat actgttttag aaaggaagaa taatagtgtt acattttgaa
     5341 ggtctgagaa aaaaagtaat aaaaaactat tttataataa ttattataat ggttactctg
     5401 tttgaaggat tgttcatgtt ttacatacaa aactactatt atgattctgt caaacaacta
     5461 cttgaatctg aaataaaata tgctgatgaa tataatgcta ttacaatgga aactacaagt
     5521 tttgagaaaa aagtaaaaaa tatatttgat aaacaaccat taactaaaaa ttcagaattt
     5581 ggaatttcaa taatagataa agataaaaat attatattag accaatatgg ttttaaaagt
     5641 aaagaaaaag caaactatga agatgttaac aatgcattaa aagatataaa aactaaaaat
     5701 ttaacacctt atacttatag aataccagat acaggggagc atgttatgag tatctcctta
     5761 ccacttaagg taaataatat aatagaaggt gttgttagat atacagtatc attagatgct
     5821 attgataatg caatattaaa gcaagctaca tggcttatat tagcaggaat atttatactt
     5881 attatagcta tactaataag tcttaaattt gctgagactc ttataaaacc attacgagag
     5941 ctaaagaaat ttgcaaatga attggctgta ggaaattata atataaaatt agaaaagatg
     6001 aagattgtag atgatgagat tggtgattta gcacagacat ttgaacacat ggcacatgag
     6061 attgacaaaa gtgaaaaatt aaaagaggaa tttatatcat ctgtatctca tgaactaagg
     6121 actcctctta catctataaa ggggtggagt gaaaccttag gttatgaaag tataacaaga
     6181 gaagaactag acttaggttt aggcattata caagatgaaa cagaaagact tataaaacta
     6241 gttgaagaat tattagactt ttcaaggctt tcttcagaca ggataaaact tcatgttgat
     6301 atagttgatg ttgaaggttt aattgtaggt gttgtaaatc aacttaaagt taaagcagct
     6361 gaaaaagata tatcactttt atttgaattt gaaaatgaat ttatagagaa cattcaagga
     6421 gataaaaata gattaagaca ggttctcata aatttaatac aaaattcatt taaatttacg
     6481 agtcaaggtg gttacataaa agttgtagct tctcaagatg aagagataac aaccatatca
     6541 gtagaggata atggttcagg tatagaaaag caaaatttaa ataaagtgtt agataaattt
     6601 ttccaagaag attataataa ggctggtagt ggattaggat tagcaattag taatgaaatt
     6661 gtaaaacttc atggtggaag aatgaaaata gaaagtgaaa agaatgttgg aacaaaaata
     6721 acttttaata taaaaaataa atttgctaaa caagcataaa ttcttagaaa gaggattaag
     6781 atgagaaaca taaaaatgat agtagcatac gatggttcaa gatataaagg atatcaaaaa
     6841 ttaggtgaca ataatatgac tattcaagaa aaacttgaaa atgttttaag taaaatgact
     6901 aatgaaacag tggagataat agggtctgga agaactgata tgggagctca tgcaagagga
     6961 caagtggtaa attttagaac taattgtatg gattctttgg ataaaattca aaaatattta
     7021 tatgaatatt taccagaaga tatagttgta aaaactgttg aagaagttga tgaaagattt
     7081 cattcaagat acaatgtaaa atcaaagact tatatgtata aaattgacaa taataagtat
     7141 cacaatcctt ttataagaaa atatgctact catgtaagta aaaaattaga tttagataga
     7201 atgagaaaag caagtgagta tttagtagga gaacatgatt ttactagttt tgcttcttct
     7261 aaatctaaga aaaaatcaaa tgtaagagaa atttattcta taaatataaa agaagatgat
     7321 aatgttatag aaatatatgt agaaggaaat ggatttttgt acaacatggt aaggataata
     7381 gtaggtgctt tgatagatgt tggtctaaaa agaagagctc cacaagatat taagtatatg
     7441 cttgaatcaa aagatagatg tcaatcatca gatactgcac cagctaaagg actatgtctt
     7501 tggaaagtaa catattagtc aattatataa aactctcttt aaaataaaaa taaaataaat
     7561 tatatagttg acataaatgg aaaaataaac tattatatat tttataagag agatataaaa
     7621 ctttatattt aaatcctgtg agaaagagag taacttctat gtgttttata gagagctgag
     7681 gatggtggaa gctcagcaaa taagtattag tgaaaagaac tttggaggat actaattgaa
     7741 tttagtatgt tagtaggtaa tctcgcctta agagaaagag tggataagtt tatcttatca
     7801 attagagtgg taacgcggat atatttcgtc tcttggtttt tccaagaggc gttttttaat
     7861 atatttaatt attagtgttt atctggtatg ttgaacattt actaattgat tagctaagtt
     7921 tatcaataag agtggtaccg cggatatttc gcctcttagt attttaaaat actaaggggt
     7981 ttttttagta ttaaaatgat tttatgtgag tctaaattct cagagtcatt caatattaag
     8041 aatttctata aaataaaaat tataatttaa gattgggagg aattaaaaat gaaggaaaaa
     8101 gtagtattag catattcagg aggacttgac acatcaatca taataccttg gttaaaggaa
     8161 aattatgaag atatagatgt tatagctgtt tgtggaaatg taggtcagga agataaaatg
     8221 gaagatgtat atgaaaaagc tttacaaagt ggagcatcaa aggcatatgt ggatgatata
     8281 agtgaagaat ttgtaactga aactatattt aaagccgtaa aagcagaggc taaatatgaa
     8341 ggaaaatact tattaggaac ttctttagca agacctataa tagctaaaaa attagttgaa
     8401 gttgctcata aagaaggtgc aaaatatata tgccatggat gtacaggaaa aggtaatgac
     8461 caagttagat ttgaagctac aattgcagct cttgacccga ctataaaagt tattgcacct
     8521 tggagaattt gggatataaa atcaagagaa gatgctatag actatgctga aaaacataat
     8581 ataaaggtaa cagcaactaa agctaaaata tattcagtag atgctaacct ttggcatgtt
     8641 tcaacagaag gtggagatat agagcaccta gaaaatgaac ataaaaaaga tgtttataaa
     8701 caatgtgtag acccagaaga tgcatgtgat gtagctgaat atgtagaagt ttactttgaa
     8761 aaaggtgtac ctaaaaaagt aaatggcgaa gaattatctc cagttgcact gattcataag
     8821 ttaaatgagt tgggttgtaa acatggaatt ggtgttatag atatagttga gaatagactt
     8881 gttggtatga aatcaagagg tatatatgag actccaggag gaacaatcct ttatgaagca
     8941 cataatattt tagaaagtgc tactttagac aaagatacat tacatttcaa acaaatggta
     9001 tcttataaat acggagaact tatttacaat ggactatggt attgtaaatt aagagaatct
     9061 atagatgcat ttatggaaca aactcaagat aatgttacag gaacagtaaa agttaaactt
     9121 tacaaaggaa atataaaacc tgctggaata tttactgaaa atgctcttta tgatgaagga
     9181 atatcttctt ttggaaatag tgaactttat gaccacaaag atgcagaagg atttattaat
     9241 ttattcactt taccattaaa aatcagagct atgaaagctg gaaaataata aaataaatta
     9301 tttaagagtg tataaaaact atttttaggt ttttatatgc tcttttttta tttgaattta
     9361 ttaaattata cagtaaatag tataaaaaaa tatattatag ttccaaatga cttaataatg
     9421 tgaaaatata agacctaaaa tataaatttt ataaatacaa tagttattga ctctctcatt
     9481 gtaagagggt ttatactgaa tctaatgaaa atttatagtg aggagataga ttatatgaga
     9541 aaaataattg aaattaaaca tcattcacat gattatgagt gtatgtggaa tggtatagaa
     9601 gatttatata taaataaaac cggtgaatgc cttccaaacg attttttctt tttactgtca
     9661 ggttttggtt cattttgtta tatgaaaaca aataaagctg atttgaaaag aatggtagct
     9721 ttgggagatg gaaggacaaa acagatgtat aaatttttag caccaattgt tggatttgaa
     9781 tacaaacatc atgagtttaa aaagtatgag caagcattaa agaaagcaaa atctgaaatt
     9841 gatggtggat atccagttat tttaggagct ttagatatgt attatctacc atattataag
     9901 aagctatatc ataaagaaca cattcctttt cattatatat taatggtagg atatgatgat
     9961 gaaagaatat atctgtatga ttgtggtaga acagaattat tacatctttc ttatgataaa
    10021 ttacgtgaaa gcatgaattg caattcccct ggattaagca aagaaaatac tatttgcaca
    10081 ataagaatga atacttcaaa gaataaatat aacattgcta gagaagctat tgctataaag
    10141 cgtgatatgt ttttaaatcc atctaaagga tttttgggat ataaaggttt tgaaaagttt
    10201 attaaagagt tgccacagtg gaacactgaa ttggataaag atgattatga taagattttg
    10261 accaatatgg ttatgttttt tggaacagta ccaacaattc caaatgcaat caaaggaatt
    10321 aaagaaccag atactgttca atttaaaggt ggatttgata aaatgagtag aatgctcaac
    10381 agtattgggg aggaatacaa aaacgactct tttatacaag tagctgatat ttttgataaa
    10441 actgctatta taattgagaa aatatccaat attataatag attatttaac tcaaacatgt
    10501 gatgatacag aacaacttcc gttattgttc tctgaggtct tagagcatat gaaaacagga
    10561 tatttaatct tggatgtata aaaaatatat tgttgaccct aacattacgt aatagtgtaa
    10621 tatgaaaata tagcttgaat atattagtag gtattgatgg ctgtaaaatt aaaatttgag
    10681 gtgtggatat gagaacagtg aaacaagttt ctgatttaac tggaataagt gtgagggcat
    10741 tacattatta tgatgagata ggtcttttaa aaccaaataa aattacagat gcaggatata
    10801 gactttatga tgatgaatct attaaaactc ttcaacaaat tttatttttt aaggagattg
    10861 atattccttt aagagaggtt aaggaaataa tgtcaagcca atattttgat aaggttgaag
    10921 ctctaaaaaa tcaaaaaaaa ttacttattt taaaacgaaa gagactggat gaattgatag
    10981 agcttataaa tcaaactcta agaggagaag gaaatattga ttttaaagag tttgatatga
    11041 gcgagtattt taatgtttta gaagaattta aaagagaaca taggaataag ataattaaaa
    11101 tttatggtag tgtagaaaaa tacaatgaat atatagaaag agttaagtct aatgaagaga
    11161 aaattgctaa aatggctata aaacaatatg gaactattga gaagtttgcc caagctataa
    11221 aaacaaattt tagcagtgat atattgaatt taggagaaaa atttgataga tataaaaatg
    11281 attgtttaaa agaaaaacat ccaaaattaa aagaattata tagaaagctt gtagaagatt
    11341 taagtaagaa tccttcttca acagaccttc aagaaatagc aaaagagata acagatatat
    11401 ctaaaaaaga ttatgaaatt ttctctatgt atactggtga tgacaactgg tattatatga
    11461 ttcaaaatta tctagtaaat cctatgtgga tagaagaagt agataaaaag tatggaagtg
    11521 gagcaggcaa gtttattgga caagtactaa agacttatct aagagataga aaaccaaaga
    11581 taaatacatt gtatgaaaaa cttgtagaag atttgagtag agattgttct tcaagggaag
    11641 ttcaaagcat tgttgaagaa atagacaatg aaatgaaaag aagtaatgaa ttttataaaa
    11701 tagacaatgg tgaaagatac tttgattata tgtcagagct ttatttacaa gattctaact
    11761 atataaaagt aactgataag aattatggtg atggtgcatc taagtttatt ggagaagctt
    11821 ttaagattta ttttgataat aataattgtt gaaaatttgg aagtaaaaaa tatgtaagtt
    11881 ttataaaagc acaaaaataa ggatgtagat tagctccatc tatatcctta tttaaagtta
    11941 ttaaa
//
LOCUS       DAEPLF010000098        11858 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEPLF010000098 DAEPLF010000000
VERSION     DAEPLF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.098"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(108..1838)
                     /gene="lpdA"
                     /locus_tag="KR314_002241"
     CDS             complement(108..1838)
                     /gene="lpdA"
                     /locus_tag="KR314_002241"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBH0328623.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEVPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            complement(2005..3051)
                     /locus_tag="KR314_002242"
     CDS             complement(2005..3051)
                     /locus_tag="KR314_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBH0328624.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYSGKIRKCDVEKLTAKETIVSNNTSKYSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            complement(3064..4050)
                     /locus_tag="KR314_002243"
     CDS             complement(3064..4050)
                     /locus_tag="KR314_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBH0328625.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            complement(4075..5037)
                     /locus_tag="KR314_002244"
     CDS             complement(4075..5037)
                     /locus_tag="KR314_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBH0328626.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            complement(5075..6028)
                     /locus_tag="KR314_002245"
     CDS             complement(5075..6028)
                     /locus_tag="KR314_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBH0328627.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            complement(6473..7846)
                     /locus_tag="KR314_002246"
     CDS             complement(6473..7846)
                     /locus_tag="KR314_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0328628.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNYIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMFFYKLDKEYSTI
                     LNELNTK"
     gene            complement(7943..9589)
                     /locus_tag="KR314_002247"
     CDS             complement(7943..9589)
                     /locus_tag="KR314_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBH0328629.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYIHSSGFDASILHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWRPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNVLGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIADESKDVD"
     gene            complement(9611..10777)
                     /locus_tag="KR314_002248"
     CDS             complement(9611..10777)
                     /locus_tag="KR314_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBH0328630.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNIVLDTHQYLMLAESDGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            10994..11839
                     /locus_tag="KR314_002249"
     CDS             10994..11839
                     /locus_tag="KR314_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0328631.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCTYQDTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSGKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
BASE COUNT         3622 a         2293 c         1281 g         4662 t
ORIGIN      
        1 aaataaatta tttgcataat aaggggtatg gctaagtaag tgttttaaaa ttttatgata
       61 taaattaata ttaataatag acttaaatta ataatttatg tttagattta ttttttaggt
      121 aagtgtaaac attctccatt tacatctgcc aaagcttcca ttaaagcttc tgaagtacat
      181 ggatgaccaa atatcaactc acttgcttca tcagtaggta tctctaatgc tttaaatgat
      241 gctgcatgat ttataagttc tgctacacca catccaaaca tatgtatacc taaaatttct
      301 ccatatttag catctgctac tactttcaca tatccttgtt cttgaccaga agctaaagct
      361 cttccatttc cagcaaaatt aaacttacca acttttacat tatatttttt tctagcatcc
      421 tcttctgtaa taccaactga tgctacttct ggaattgtat aaacacaact aggtaatgca
      481 cctaagtcaa cttctttatt cacacctaga gcatttgaag ctgctacctc tcccatttta
      541 aaagcagcat gagctaacat tacacctcca gtaacatcac ctactgcata tatgcttggt
      601 atacttgttt ccattttact atttacaact attgaacctt tatctatttt aatatctaag
      661 tcctctatgc cagaaagatt agcttctctt cctatagcat atagacataa atctgctttt
      721 attggcactt caccttcaat gcacactaat atgttatttc cttcttcttt aaattcagaa
      781 actttctttt tagttaatac atttatacct tttttactaa gagaatattt taaagattcg
      841 cttaactctt tatccattct tgggattact ctatcttcca tttctactat agttacttta
      901 gaacctcttg aattgaatat ctctgcaaat tcacaaccaa tcactccacc accaattatt
      961 actaattctt ctggcactgt ttccaaatct aatgcttctg tactagttat tattaaattt
     1021 gattctattc cttttatagg taatatcctt actttggaac ctgtagcaat tattatattt
     1081 tctgtatcta aaacttttcc atctgataaa attactttat gctcttcttt aacactagct
     1141 ttaagattaa acacatctac atctctactt tttaataatc cgccaacacc agctgtaagt
     1201 tttttaacta ctctattttt atattttatg gccttcttaa tgtcctgttc tttatctaca
     1261 gttactttta cacctctctt tgataattgg tcaatttctt ctaatatttc tgctgttttt
     1321 atataagtct ttgtagggat acaacctcta tttaagcaag tacctcctag tatattttct
     1381 tcaactaaag caactcttcc acctaaaaga gctgctttta atgctgataa atatccccca
     1441 ggtcctcctc ctattaccac tacatcataa tcatgattac actctttttt gtttattttt
     1501 tcttctttaa cttctatatt ttgattctct tcaactatat cttcttttga aacatttcca
     1561 gataaatatt tagcttttac ttcctcttga ttttcccctt tttctgctat tacacctatt
     1621 actgtaaata ctggtaatac ttcaccttcc tctttatgta tgattacagc aagtgtacct
     1681 tctccttctg actctatctc catattaact ttatctgttg ttatttctag tataggttct
     1741 ccaatcttta cttcttcgcc ttcttgcttt agccaagata ctatagtacc ttcttccatg
     1801 gctactcctg cttttggcat tattacttct actgacatag gctctcctcc tatttatttt
     1861 catatatttt aattaagcaa atcatattca catatagtta ctgttaaaaa taattcttaa
     1921 tatataaaag ctttaaattt actgaaacaa ttataataat ataatctatt caaactattt
     1981 atatcagtaa cgaaatatca aaacctatac aagcattgat aaaggatttt ctaacaattc
     2041 ttttaaatct tttaagaatt ttgcagctac tgttccatct attactctat ggtctgatgt
     2101 taatgataag ttcattatag gttttatttt agcctctcca tttactggaa caaacttatc
     2161 ttgtgtagca cctaccccta agattgcact tgaaggcata tttattatag gtgtaaatgt
     2221 agttatacca tacattccaa cattacttat tgtaaatgta ttaccttctt ggtctgctgg
     2281 cataagtttc cctgtcttta ctttttccgc aagttctttg ctttcttttg ctatttcttt
     2341 taaagacttt ttgtttgcat tttttactac cggaacatat aagccttcat ctaaaccaac
     2401 tgcaatagct atatttacat ctttatatct aaatataccc tcatctgtcc atgctgaatt
     2461 taaggcttga tgatttggaa gtattttaga tactgccatt actattaaat ctgtcatagt
     2521 taattttact ccagttgatt cttttactgt gtcgattaac tttgctctaa gtaccttcaa
     2581 ttctgtagca tctacttcta tattgaatgt aaatacagga gcactaaaat aactttctga
     2641 cattcgtttt gctacagtag ctctcattgg atttggcttt tcaaatattc cttctactgt
     2701 gttaaacatg cttgaatttt cattttcaat tttcagctct tttttctcac tatatttaga
     2761 tgtattattt gatacaatag tttcttttgc agttaatttt tccacatcac actttcttat
     2821 ctttccacta tatccagtac ctacaatagt ttctaaatct atatttaaat cttctgctat
     2881 tcttctagct aaaggagtag tatttgcttt attatctttt aaataattta acacatctac
     2941 taaatgaatc cttccatttt ctccacttcc aatcagccta tctaatttta tattgtcttt
     3001 tcttgcctga cttctagctg ctggtgtggc ttttatttta tttgccacca tatctatccc
     3061 tccttatcta acaattaaag attttattgc ttcttctatt tcatcaactc ttgggacaac
     3121 tgctttttct aactctggat tatatggtat tggcacatct tttccacaaa ttctttttac
     3181 tggagcatct aaataatcaa atgattcact ttctgttatc agggctgata tttctccacc
     3241 tagcccacca gtcttagctg cttcatggca aattaaaact ctcccagttt tacatacact
     3301 ctttactata gtctctttat ctaatggata taaagttctt aaatctataa tttcaacatt
     3361 tatattttct tttgatagag tctctgctgc ttcttcaaca ctttgtaaca ttcttccata
     3421 agtaattact gtaacatcag taccctctct tttaatatct gcctttccaa tttcaattac
     3481 ataatcatct tctggcacaa aacctttttt tctgtataac aatttattct caacaaatat
     3541 gactggatta ttatctctta tagctgcttt caacaatccc ttagcatctg ctggtgtaga
     3601 aggtgcaact acttttactc ctggtacatg acaaaaccaa gcttctaaac tttgtgaatg
     3661 ttgctctgct gaaccagttc cagaaccacc tggacatcta acaaccattg gaacttgtgc
     3721 ttttcctcca aacatatatc tcatttttgc cgcttgattt acaattgcat ccatagaaat
     3781 tgttacaaag tccataaaca ttacttccat aataggtctt aaaccagttg ctgctgcacc
     3841 tgctgccgct cctgctatag ctgcttctga tataggagta tcccttactc tttctggtcc
     3901 aaattcatct atcataccaa ctgatacacc aaatgcccct ccatatattc ctatatcttc
     3961 tcccataaat attacatttt catctcttct catttcttct gacattgctt cctttattgc
     4021 ttgtgcatat gttaattccc ttgtactcat ttttattttc ctcctttacc attcttagtc
     4081 tgcatataca tcctctaata aagactctat ttttggattt gggctatttt gagcaaactc
     4141 tactgcatct tctattgatt gctttgcaaa ttcttgaatc ttatctaatt catcttcatt
     4201 agatagatta ttttctataa gataattttt taaaaattca atagggtctt ttgctttcca
     4261 tgattctatt tcttcttttg ttctatatac atttgcatct gattttgaat gacctaacca
     4321 tctataagtt ctactttcaa ttaatacagg tccttctcct cttctacact tctcagcagc
     4381 tttttgtact gtttcgtata cttctattgg gttatatcca tctattgata ttccttctat
     4441 tccataagaa gctgctctag tggcaatact ttctatattc atatgacgtt ttatgcttgt
     4501 tgacataccg tataaattat tctcacagta aaatattatt ggcaatttcc atattgaaga
     4561 taaatttatt ccttcatgaa aagtaccttc atttgatgct ccatcaccaa aactacataa
     4621 aactattttg cctgtttttt tatactgttg tgtaagtgca gctcctggag ctattgtaag
     4681 acctccacct acaactccat ttgctcctaa gttacctgac tcaatatctg ctatatgcat
     4741 tgagcctcct tttcccttac aaaatccagt ttctttaccc ataagttcag ccatcatttt
     4801 atttaggtct attcccattc ctataaattg actatgtcct ctatgagtta atgatactaa
     4861 atctcctttt tctaaagcca ttacagccgc cacacttgac gcttcttgac ctacagataa
     4921 gtgagttgtt ccatgaacca tacctcttgc aaaaaaccaa ctaactttct cttcaaattt
     4981 tctagcctga ttcattctct tatacatttc aagcaatgtt tcttttgata ttgacatata
     5041 cattccctcc tgttaacttt tatctatctt tctattagtc acaatcttct aataatttaa
     5101 gtgctaaact gtgattaatt gctaaactat ttataaattt cgcttttaat gatgccttta
     5161 ttgcctctat tttttcatct cctgaagcga cacctattgt tctttttatc ttttttaaat
     5221 cttccagttt cactcctaat actctttgat taaattcaag tatagaagtt tttccttctt
     5281 tatcaaaggc ttgtaaacaa attgcaccta ttgagttgta tttttttaaa tcttctatat
     5341 ctgcttcatt catgtatcca gaagccatta ttgttgagtt taaactcata ggatttccta
     5401 tacccacaat tgctatagtt gttttatcta ttaaatctag tacttcttta attttttcgt
     5461 ctttgcaaag tttctctttt gtagacaagt cagacataac agctggagca tgtaataatt
     5521 taaaatcacc tccaaaagcc ctcgctagac ttactgctat ttgatttgca tgaatatcta
     5581 tttcattgtc tccaatccct cctaatagag gtataaatgt aacattttta cagttacttt
     5641 tttccacata tcttggtatt tcttttagtg ttgttcctat tgaaacacct attatatcat
     5701 tttcctttac tactctagtt aaatattcag atacagctct tgctagttct tgtttttgtg
     5761 ttagtgcatc ttgtttatca tctactacta ttacttcttt taatttatat tttctctcca
     5821 aacttctctc tattttttgg taatcatttt tcaaaacatc aactatctgt atttttacta
     5881 ttccttgttc aaaagcttct tttaatattc ttgatattgt tggtcttgat attcccaatt
     5941 gatttgctat ttcctgttga ttcaaattat cttcgtaata aagtgaacaa cacttcatca
     6001 ttaatcttaa gtctccaatt tttttcattt gatttcctcc aaaatcaagc ttttcaatta
     6061 tctttacatt tgttcaatca ttttacttaa tttcagtaac attatagaag gggttcatat
     6121 aaaaagtcaa ctgttttttt gtataacaaa aaaaaggtga tacttttctt gtaccaccac
     6181 cttcataaat agttgaaaaa ttgcttctta gcgcatatta tatattactt ctaattgtta
     6241 aatatcatct ccataaatta aaaattaatt gtccacttat aatgctttaa ataaaaataa
     6301 cttattaaat catttctaaa agaccaacca tttcatcatt taaagtcttc ttatattttt
     6361 attattcgct tctctaaaaa ttcttcaata atcataaata tataatagtt tttataggga
     6421 acatttacaa tagaccttaa ttaaacttaa gactttataa gaaacttttt cattatttag
     6481 tatttaattc attaagtata gtactatatt ctttatcaag cttataaaaa aacattatga
     6541 taatttgtaa tattgttata aatataggta ggtatataaa acatactttt attgatacta
     6601 tagctaaatc actttgagta gctgatgttc ctacataacc accaattgat aatactgcac
     6661 ctaatattgc tgaacctaat ccatttccca ctttaaaccc aaaactagat gcactattta
     6721 taagcccctc agttctatat ccagtcttcc attctccata ttcaatagta tctgaaacaa
     6781 tagcaaacat tgttgatgct attccagcac ttcctattcc ctttatagca cttgcaaata
     6841 ttactcctat atagttttgt ccaactatta taaacataac atttgacgat attaatatta
     6901 ttgaacctac tattaataca tttctttttc caaatttttt tataaaagga gcaaccataa
     6961 acatagctat aatttgggat atatttgaaa ccatatttat aggaccaaat aatttttcat
     7021 ctcccaaaat aaactttgca taatatacgg atgaaccacc atttatcgca aatacaataa
     7081 atatgcaaac taaacataat gttatttgta tccagtactt atttctaaat aaagatttaa
     7141 tacctatttt aaatggtatt acttcattcc tatcttgctt aactgctttt actctttcct
     7201 tggttccagt aaatgttata aaaaatacca ttatagatgc tattccaaat acaaagaatg
     7261 ttttagtcca tgctgataaa ttatttccaa agtattcaac tagaggtagt gtaagtccat
     7321 ttattagtaa ggtaccacaa gtggctaata tcattctaaa tatacttaat actcctcttt
     7381 catactggtt ttgagttata agtgaattta gagttgcata gggtacattg attgctgtat
     7441 aaataacagt agaaactaag ttatatgtaa taaaaatata aattaattta gaagtgatac
     7501 caagatttga tggaacagaa aataataata ctgctgctat agcaaaagga atcgccattc
     7561 tcaaaatcca aggcctagcc ttaccgtatt tggatttagt tctatccact ataattccca
     7621 ttgttaagtc actaaaccca tccagtatcc tagatacaag cataatacta cctacagctg
     7681 ctgcactaac atctgcataa ttagtataat aaaacagcaa aaatgctgac atagctgagt
     7741 atatgatatt gcaccccaaa tccccacaac cataagaaat tctttcaaaa aatgaaatct
     7801 tttcatatct aatcgtagtt gctttaacat taacttctgt attcataata ccatactcct
     7861 ttttgtaaga tttgccttct aatgtttctt atttcagagc ataagattta cctttgctct
     7921 gaaaatttta gtaatgattt tattaatcga catcttttga ttcatctgct atatattcaa
     7981 aaaaatcaaa atcagcatag tgtttatgct ttacacgatc tgcacaagtt aatcctataa
     8041 acgtacctgt aaattctcca tatttacagt actcatctga aaacttagtt gtatcaaata
     8101 cctttcctat tctttggtag tttttttcat cataactcca ttcaaaatat gacttacgac
     8161 cttgtatata cagacgaaga tatattggta tatcttttat tggtatccta gtatttaaaa
     8221 attccgtctt ctctccattt tcaagatgta ttattgatag tgcactttga cccaatgttt
     8281 cactataata ctttcttaaa tttatataat tcatattatc ataatacatt atcaaacccg
     8341 cactatgctg atgtacctct gggtaaaact ccatcttagt ggttattctt gcatatactg
     8401 aagttaattt tcttgccaat atgcttactt tatttaatga tgttcttgat tcttgacctc
     8461 taatccttac atatcctggt ctagacttaa catctgcaaa tgataatggt gaaatcctag
     8521 gagcatagta ctgtaaacct aatacatttg aatcaaagtc atcaaaactt ggtatagatg
     8581 aaactaaatg ttcttctagt ttactttctg atacgtattc ttttgccaaa ttactttcat
     8641 cttccattcg aagccaatta tcctttgtcc atttcatttt ttgaatagct gtttctctac
     8701 ctaaagtaca ccttaactct ggtacaaatg gtctagatgt tagatgaact aaatatactt
     8761 cacctgatgt tgtttctata tagcttccat gaccagattt ttgaagtttt gattctggat
     8821 tatagtattt aggctttaga tggtcagggt catgtctttc ataaaaatcg cctggaattg
     8881 aagttacaat tggattcatt gaatcttttt cataaggtct ccatatattt ttagcccttc
     8941 ccatagttac actatgacca tatccagttc caccttctgc acacattata taatagtaat
     9001 ctccacgctt tgttatatgt ggtgcttcaa tacaacctct atcagttccc cctgaccata
     9061 ttctctttgg atatcctact atttctttct tttttgtaca gtattccact aaacatataa
     9121 caccaggttt ttcatatcct tctctagtct cccagtctaa tgatgcaatc cactttttac
     9181 catcatcatc atgaagaata gacgcatcaa aacctgatga gtgtatatat actggttcac
     9241 tccactctcc ctttatatct tttgatgtaa ttaaataatt atcaacatca aaatatcttg
     9301 cattcataga gttcataatt ccataaacta tgtaaaataa atcttcttct tcacaataag
     9361 tcaaacaagg tgcccaaatt cctttagatg aaggaagctt cttcaaatct atttttgttt
     9421 catctgtcaa aatatgagta tataactccc aattttttaa gtctttagag tgataaacag
     9481 gtatacctgg aaaccattca aaagatgaaa ctacaagata ataatcatct ccctttctac
     9541 aaatacaagg atctggatta aaacctggaa gtatagggtt tttaatcata tcttttcctc
     9601 caattttagt ttaatattct ataggatacc attcttgagc tacacatttg cctaggtccc
     9661 aactatccca tcctatccaa ccttcttcat taactgtatc tagtaataac ttataattcc
     9721 aatagtagtg accacttcca tttctccatg catcaagctg tgcttttgct atcttcctat
     9781 ataattctct tttatcgtct tttgataatt tatctatatt tgattcaatc ccatttagag
     9841 gtgattgacc tccatttgta tctataccac aagcatatga attaaataag ctccactccc
     9901 cacatataac aggaaaatat ttttgcattt gtaaaatatc tttagcataa ttttctctaa
     9961 tatatttgag atagctatca atactttgtt cacatccatc tgattcagct aacatcaaat
    10021 attggtgtgt atctagaaca atatttttaa attcttcctc tctcataaag tctttccatg
    10081 cttttaattc aaatgcatca tgaaacacta taacgttttc ctctctcata taaggacgca
    10141 ttaccttata tgcttttata taaaaatcac gtaaaaactc taatgaatta ggtttactcc
    10201 cccttgccat ctccttatct acagccttaa accgatttgg aacattcatt atgttccaca
    10261 ttctttctgt tataggctca tttaaaattt ggattccata caattcatgt ctcatcccat
    10321 aacgttttgc aagtctttct aatacattta atgtaaacgc tacatattct ggattttgtg
    10381 accagctaca tatacctgat attcctccat tatcaaatcc attttggctt cctggaactg
    10441 tatgtaagtc tatcaatata ctaagcccat acttatctgc ccaagcaaat gctttatcaa
    10501 gttctttaac acatcctatg aatggttcac aatctccaaa aataaagtat ggtactggta
    10561 tacgaactga attaaatccc atagatttaa tcgttgcaaa atctctctct gtgatgtatt
    10621 cactacgatg agtcttaatt ctagattcat atgcctctct tgaaagctgt cttggtaaat
    10681 agtattcatc ttcagcttct gtgccttcaa ataaacttga ggtcatccat ttttctaaaa
    10741 ctaaccaacc tcctaaattt acacctttta tatacacata aaatcctccc ctcttatctt
    10801 atttaaatag gtatattttg cacttataat aaaataattt ttttaagtta ttttaatata
    10861 cagttattat atttgatttt atccgttttc ttaagtatgt ttattatcta atttattagt
    10921 ctatattatc tttttaatat tgaacattat aaatttacta tttataattt aaaattataa
    10981 ggaggatatt attatgaaca gaaataattt ttcacatgaa ataattgaac ctattgataa
    11041 tttagatgtt aatttcaaac tatttgatga tagtggtagt tatgtagcaa atcattggca
    11101 taactcatta gaaataattt atataacttc tggtgattta caaattaaca tggagggtta
    11161 cacttataat ttaaaagcta atgagtgtat gttcattaat tctggaatta ttcactcaac
    11221 tagatgtact tatcaagata cctctatatt attgcaagta ccattgtctt ttctaaacaa
    11281 atatatccct gattttaaaa attgctattt tgactttaag gttaatgcca atgacaataa
    11341 ctataaaaaa aattcaggta aagttaaact tatattagaa aacatgagag aaattaaact
    11401 tagctctcct cctgcagcta atctgctctt tacaagctta ttatttgaac ttttatttga
    11461 attatataca aactttaaaa tatctgtagg taataaaaat atgaaaaaaa cagtacttga
    11521 tttgtcaaaa tttgaaccag ttttagaata tacaaagatt aattacaaaa tgcctatttc
    11581 tattaacaaa atcgctaagg tagcacactt acagccagaa tatttttgta gaaaatttaa
    11641 acagtatatg ggacaaactt acttagagta tttaaatgat gtgcgtattt ctcatattta
    11701 taaagactta attaacacaa atgatacctt atgcagtata ttagaagctc atggatttac
    11761 taatacaaaa ttattttaca gaatttttaa ggaaaaattt aaatgcactc ctaaacatat
    11821 aagaaaaaaa tctacctaaa atatgaggtt atatgtta
//
LOCUS       DAEPLF010000099        11821 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEPLF010000099 DAEPLF010000000
VERSION     DAEPLF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11821
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.099"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(97..1220)
                     /locus_tag="KR314_002250"
                     /pseudo
     CDS             complement(97..1220)
                     /locus_tag="KR314_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
     gene            1417..1641
                     /locus_tag="KR314_002251"
     CDS             1417..1641
                     /locus_tag="KR314_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328632.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            1694..2218
                     /locus_tag="KR314_002252"
     CDS             1694..2218
                     /locus_tag="KR314_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0328633.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(2323..2469)
                     /locus_tag="KR314_002253"
     CDS             complement(2323..2469)
                     /locus_tag="KR314_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328634.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(2608..3534)
                     /locus_tag="KR314_002254"
     CDS             complement(2608..3534)
                     /locus_tag="KR314_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBH0328635.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            3725..5596
                     /locus_tag="KR314_002255"
     CDS             3725..5596
                     /locus_tag="KR314_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBH0328636.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNASCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(5688..6623)
                     /locus_tag="KR314_002256"
     CDS             complement(5688..6623)
                     /locus_tag="KR314_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBH0328637.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            7143..7655
                     /locus_tag="KR314_002257"
     CDS             7143..7655
                     /locus_tag="KR314_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBH0328638.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKIYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            complement(7810..8871)
                     /locus_tag="KR314_002258"
     CDS             complement(7810..8871)
                     /locus_tag="KR314_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBH0328639.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(8995..9888)
                     /locus_tag="KR314_002259"
     CDS             complement(8995..9888)
                     /locus_tag="KR314_002259"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBH0328640.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIHSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQNYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            complement(9898..11379)
                     /locus_tag="KR314_002260"
     CDS             complement(9898..11379)
                     /locus_tag="KR314_002260"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBH0328641.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKKFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     misc_binding    complement(11487..11718)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         4465 a         1519 c         1663 g         4174 t
ORIGIN      
        1 attatataag attgcttgaa gttaaataat ttatttaaat agaaaacagc tgatttaata
       61 tcagctgtat atgaataaaa ttttgaattg tagtgtttag ttatatggat ataattcata
      121 ataagttagc aataaatcaa ccattttacc ataactctga gtaccttcag taacgccatt
      181 agcttttaag taagaattgt taaactcatt agaaatttca ttaattttac cttcatattt
      241 ttgccaaaat tcactttcat tttttaaatc tctacggaca gaatctgaaa tgccagcaga
      301 aatatcaaca tatgcattat aatcaacctt acttaaagca gaagcagtat aatttaaagc
      361 taaaatatat ccagaatagt taaaatcaat atgggggtgt tttatagaag taagatacgc
      421 tataaaatta gcctcatctt cactagcaaa tcccctttga tgtgctattt catgttcaac
      481 tgtacaagga atatataagt caggtattgc tatatttaca tttgcctcac ctgtaaaagg
      541 aaagtatata ccagttattc ctgtatagca cattagattt gatgaaataa tatacttagg
      601 cttagaataa ctaccactaa cactaggtaa tatatccaag atattatcgt atcctagttg
      661 agctctattt atgacaccat tataatctgt attagatttt acaataccat ttttatcttg
      721 taatgtgagt tttctagttt catttgcttt aattacaagg aatttatata atttagtaag
      781 ctctttaaca ctatattgtt ttgattggat agaggtattg ttttttaaat tatagttatt
      841 tataagtgtg gtttctagtg gaatcctatt atagtttatt ccccaaagca ctacaaaaag
      901 aaagtagact attgatacaa tggataatat atttaaaatg gaatttttta aaaatatgtt
      961 taattttttt tcttattaaa aattgtagat aaagtacaac ataaaaataa gaagatagat
     1021 ataactatta tgtacattgt aatctcatag agagaaaatg ggaaaattcc agaaacttta
     1081 ctcaatattt gtacaattat tttatctatg aattgggagt aatatttttc aacaatattt
     1141 ggaactttgc tggctataaa atttaaaagt aaagcaatag gaaagagaat taaagaaaaa
     1201 tattttgttt ttttgctcat atttcacctc acatacatta gtttatataa acatattata
     1261 tatcaattga agaaagattg taaaataagt ataaatatat ctatttataa ctttatacta
     1321 aataaatgat aagttataat atattaatat gaataagcag tatatataca actaataata
     1381 agaaaaatat caattatata aggagtagat aaaaatatga taataaaact tactaaagac
     1441 aattttaatt ttagatttaa acaatatgaa ggtatgtttt ttcatgctga agtagttgaa
     1501 ggtatggaat ccattagata taaaataact aaggataaag aaggtacaga attaaaagaa
     1561 tttggaagta cacatataaa agataatatg atatatgtac ctcaggtaga agcatacata
     1621 gatttgaaca gtataaaata aagatgtaaa attttaatta aaaataaaga ttatgtttga
     1681 gggggaatcc aatatgagta agcctataga tataacaata agacctataa gaagagaaga
     1741 tgcacaagct tttaatgaaa ttagaagaac atacagtgtt atgagaaata ctttggcatt
     1801 aatgagtgac agagtcgata aaactgaagc tatgttagct tctttgggag aaaatgatta
     1861 tatgtttgta gctgaaacta ataataatgg agaaaaagat gtaataggtt ttgctgggtt
     1921 acatgtaaat tcttctccac gattgagaca tagtgcagaa ataggaataa gtgttgcaga
     1981 gaattatcag ggaagaggag taggtaagaa gcttatggaa caacttttag atatagcaga
     2041 taattggatt atgttaacta gagtaggatt agaagtaatt gtagataatg aaaagggatt
     2101 aaatttatat aaaaaactag gttttgaaat agagggaact aaaaaatatg ctgtaatcag
     2161 agatggaaaa tttgaagatg tttatattat gggaagatat aataaaaatt taatttaaag
     2221 aagctaatag agaagtaaaa tagagtgtct ttaataaaaa atttacatta aaaataatct
     2281 attttacttc tctattgtat taaaaaactt atttttaaat gtttacaaaa acattctata
     2341 tacaaataaa attattagca atatcaacgc tattttaaaa gctaaactag caacagtttt
     2401 aatgattttt acagaaacac ctatagcaac tatagcaaat actattttaa agagtaaatc
     2461 catatccata tttaagcctc ctattctata atacatatag atattattga caaatatcta
     2521 ggaaaaaatt ccatcaatat actacaataa aatgtttata tttgtaattt attgaatatg
     2581 ttattatata atacataaaa aaataaatca agataagagt aaaatactta taaaaggtat
     2641 agatataact gaaaatagtg ttgtaaaaaa cacacattgt gaagctagtg ttatattgga
     2701 gtcatattga ttagccatta tagcagtatt tgcagcagca ggcattgaag aaattactac
     2761 tggtatagct aaaatcatct tatcatttat ccatcctttt agaataaaat acacaatcac
     2821 aggaagtacc aataatctta taaaagtgac tatatataat tttttgttga caaaacaatc
     2881 gagagctgaa gaattagcaa gtaagcttcc aatgataatc atggatattg gtgtagttat
     2941 acttcccaac atctcaagtg gctcgtttat aaattggggt aacctaagtc ctgttacaaa
     3001 taaaaaaagt ccaataacaa tagctattgt agcagggctt ataagtgatt ttattgaaaa
     3061 acctctactt gtactgcctt tagataaaag ataaattcct aaagtgaaag aaaataagtt
     3121 aaaaggcaaa ttgaatattg cagtatagaa tattgcttca tgacctaaaa ctgcttcaat
     3181 tacaggataa cccataaaag caacatttga aaaaacaact atatactgat atattcccaa
     3241 atctttttta tcattacatt ttaaaatgta tttcaataaa aatgcaatta taattgaaac
     3301 tatatacata atcaaagatg taaataataa caatagtatt ttttgtatca ttttactatc
     3361 aaagttaatt tgcatggagc taattatcat tgatggtaaa aaaactgtca ttgtcaaagt
     3421 tgaaagctta gatgtgcagt ggtcatctaa taagttgaat tttctgacaa aatatccaac
     3481 taaaataatt ataaataaaa ctgcaacttg gataaaaata ttgctgaaat tcataaaatg
     3541 tcccccccta tatatacatt atataccaat tacataataa aatctaatga ttataaaatt
     3601 taaaatttaa tgttacaata tagtgacaaa aggtaaaaat tcaatattat agcattaatt
     3661 atagattata atgtataata atgtttgaga gaatatattt tataaaatgg gtaggtagcg
     3721 aataatgaaa aatacaaaat tttctataaa aaaaataaaa aatatatcta atgacccgaa
     3781 agtcgtacat actaagggtg ccctccttga aaagatgggt aagatggaaa gcgcaataga
     3841 gttgtataag agtgctgcat tggataatta cgtaaaatct caatatactt taggaaatat
     3901 atatgaaagt aaaaaacaat ttaaagaagc agaaaagtgg tattcaatgg catataaaag
     3961 tggaagtgaa gatgctgctt ttgatttagg taatatgtac tataagttag atggctatga
     4021 atatgctata tattggtatg aaaagatagc aaatttaggt tatttacaag cacaaaataa
     4081 tttaggtgta tgccatttta aaatgaaaga ttttgtaagg tgtgaaaaat ggttaaaaac
     4141 agcagcagac aagaatttag gtaaagcttg ttttaattta ggggtattat atactttttt
     4201 ggagaaagat gatgaagcat atgaatacta taaaaaaggt tctgtgcttt tagatgatga
     4261 tgcaaaatat aatttagcaa tattaaatgg aaaaaagaaa gataataaat caacagtaag
     4321 tttatataaa cagttgtaca agtcaggtca tatgaaaggt tgctttaact tgggtatgat
     4381 tatggaaata aatgataatt tagaagatgc agagaagtat tataaaaaga gtgctgatag
     4441 agatgatgtg aagtctgagt ataggcttgc atatgtatat gatagaaaag aagagctggg
     4501 agatgcaatt tattattatg agaaagcaat agaaaaaaat catacacttt ctaagtatag
     4561 acttgctaat ttatataata gagaaaatga gatagagaag gcaaaaactt attataaaat
     4621 ggctgctgaa gatgatataa cagaagcaaa aaataatctg gcaggtatat attttgaaga
     4681 aaaagattat gaaaatgcta ttaaatatta tgaggatgct atagctgttg gatgcaaaag
     4741 ttcccttgaa aatcttgggg atttatatta tcagaatcaa gatatagaaa aagcgatttc
     4801 gtattattcg aggattccaa ataatgcatc ttgtcaaatc aaattaggaa atatctatga
     4861 agatttaaat aatattgaag aagccataag ttggtataag aaagcatctg aaaatggaga
     4921 tacgaggtct agttatagat tggggtgtat atatgagagc ttaggaaata caaaaaatgc
     4981 tagaaagtat tttgaaatgg caagtagcaa aaatcatatg aatgctcgta tacatctagg
     5041 tagaatttat tttagagaag gtaaattgga agagtctaaa atgatgtttg atacacctgc
     5101 caatgaaaat aatgtgtacg ctcaacatat ggtaggatta atttatgata tgttctataa
     5161 agattatgta aattctaaat tttggtatga aaaggctaga gcacaaggtt gtgttgaatc
     5221 aatatataat ttaggccaaa tttatttaaa attaaatgat gacgcagaag ctgagaaata
     5281 ttataaagaa ggtattaaat atggaagtaa gaagtgtgag tatatgcttg caggtcttta
     5341 ttataaaaag agcctagata tgtacatttc tttagcaaat gaagagtacg ataattgtga
     5401 agagatagta gaaggctttc ccaatttaaa tatagacttt gatgaagtat taattcctcc
     5461 atttaagttg caagaagagg taatagatga agaagagtat gtaccaattt atatactaaa
     5521 tataaaagaa agtttagatt caatgctaga agggcttgaa actgaaatga ttatagatga
     5581 agaacatgat agttaaaaag tattataaga ttatatagat ttaaatatat aaaagccatt
     5641 aggataaatg taaatcaatt aattctaatg gcttttttat taatatttta taatagctta
     5701 tttcttgaca tgaaaaatgt aatcactata catattatag aggtaataag aagtattatc
     5761 caaaatccgt ttgggttatt agaaaatggc atgttgatga cattcatacc aaagaatcct
     5821 gatattattg taggtattga gaaaattagt gtaactactg ttaaaatttg cataacatta
     5881 ttctgattat tacttataat tgcactgaaa gcatccataa ttctactcaa tatatctcca
     5941 tatattttag ccatatcaag agcctgtcta ttttcaacta taacatcttc taataggtct
     6001 tcatcatctg gatacttctt tataccagca gttctaacca ttttgtttaa aacaagttca
     6061 ttagatttta gtgaggtaga gaagtatacc aaagattttt ctaattccaa taattgaacc
     6121 aattctttgt ttttcattga tttgtaaatt tctcgctcta ttatgattgt cattttattt
     6181 atttttctta agtaatgcaa gtaataagct gcatttttgt gaagaatttg aagcaaaaag
     6241 cgagtctttt taaacgtaaa aaaatcttta atatgaccaa ctataaagtc atttaaaatc
     6301 ttagtctgtg catcacaaac tgttacaata gcttcttcag ttagtataat acctaagggt
     6361 attgtagtat aatgagaaga attagagtca ctttcgtcta ctggtatatc tataagtata
     6421 agagtatgat tgtcttcaac atctattctt gagcgttctt cttcatctag tgcagattct
     6481 atgctttcta catctatatt taataaatta gaaatttcgt tgatttcact atgattaggt
     6541 tcaacaagat ttatccaaca gccatcttca aaaaaactaa gtttctctaa ttttgaatct
     6601 atagttttgt aatatcttat catggcaaca catcctttat tcagttttat ataaatctca
     6661 tcatttaata gtatctatta attaactaat ttatataact gaatatatgt attagttttg
     6721 attttagata ttattaagtg atgaaaatgt aaaaaattct ctccgcaacg gactagtatc
     6781 cattgcccat cactctcctt atttattatt ttatatatag ctatttatga aatgcgtatt
     6841 ttaagcactc tttaatataa taccttttcc aggtatataa atcaagtttt gatttgatta
     6901 aataatattt ttaccataat ttacacataa agtatataag ttatatttaa tttgagtaaa
     6961 gaatatatat attctgagtg aaattattaa atatattaaa aaataagagt gtatatatat
     7021 atttacttat ttgcaataat atatttaaaa aagtatttta ttttcaaaac tagacttcat
     7081 ttataattta tcacgaaaat aaaaggaata taaatataag agaatgagag gttattagag
     7141 atatgagtga ttttaaagaa attttggcag aagagattaa aaataatcca tttggattga
     7201 taggtaaaga ttggacactt ataacagcag aaaatgaagg taaagtgaat acaatgactg
     7261 ctagctgggg aggacttgga gttatgtggg gaaaagatgt tgcttttgta gtaataagac
     7321 cacagcgtta tactaaggaa tttatagata atacagataa attttctctt acattttttg
     7381 atgaagattt tcgtaaagag ctttcatatt gtggaaaggt atctggtaga gaagaggata
     7441 agatatcaca gattggattt aatatagaac acttaaatga tacaccttat tttaaggaag
     7501 ctaaaatggc tattatatgc aaaaaaattt atagtcagaa acttgaaccc caatgtttta
     7561 ttgctgaagg tatagatgga agatggtatc cacaaaaaga ttatcatact ttatatatag
     7621 ctgaaataac caatgtactt gtaaaagaag attagttata agggttaata ctatctctaa
     7681 ttaaattaaa atttcaataa aaggaaattg acacaaaaaa gctaaggaca taaatatgtt
     7741 tataaagatt aataaataac gtattaaaac tccttagctt gataaaattt taatttagtt
     7801 ttctttaaat tataattcag aaatcaattc attgataaca tcttcaacag tacttaattt
     7861 atcaatttta taggcatcat ttccacaaaa taaaagtgaa ttttcaacat ctcctttaac
     7921 tgcattgatt agagcttgag aaatacagta aggagtttct tttggattac aaggaatcaa
     7981 acaattatag cacttcttta tttctggacg atttatttta acttcttcta taaatttgtt
     8041 tcttatggcg cgtccaggaa gacctacagg acttttaact atatgaacat cttcttcact
     8101 agcatttatg tatgccattt taaagttctc atgggcatca cattcgtaag tagcaacaaa
     8161 tcttgtacca acttgtacac cacttgcacc catatctata tattttttaa catcactact
     8221 agaagttata ccaccagcag caactactgg tatgtttcta ccaaattttt ctccataagt
     8281 atttgcaacc tttaaaattt caacaaattc cttatcatag tctatagaat ctatgttatc
     8341 aagttcctcg ttagagtatc caaggtgacc accagctttt ggaccttcaa caactattaa
     8401 gtcagcagta gttttctctt ttctatccca catttttaaa attacattag cagcttttgc
     8461 agttgaaact atgggtgcta tttttacatt acttcccttt actaatgatg gtagcttatt
     8521 aggtaatcca gctccagata ttataaggtc tacaccagct ttaacagctt cctttacgta
     8581 tgtttcatat tctttcatag ctaccataaa attaatacca ataataccac catttgagat
     8641 ttctttggcc ttagaaatat gtttttttat agctcttaaa tttgcattta tagtattagt
     8701 ttcaaaatct tcctcatcat atccaatttg aactccagaa attacaccaa caccaccaag
     8761 cttagcaact gcaccagcta gtgatgacct agatatacca acgcccattc caccttgaat
     8821 tataggaact tttgcaacta aatctctaat tattaactct tttatgataa tccctcctca
     8881 atgtaaacat gtttaaagaa ctagattata atacttaata ttattaccta gtcctaatta
     8941 ttatactaca aattataata aaagagtagt tatattttgt atagaagacg ataattatat
     9001 tatatttaaa acagctccat tagaatcgca acataactct tttatttccc aatcagattt
     9061 tagtttttgt aggtaatttt tcatattatc cacaaaacta tcatcatttt cttttattaa
     9121 agacattata gtaggtccag ctccacttaa aaatactcca atactattta attgttcaga
     9181 tttttcaaca atatcattgt agttctcaat tagagttcct ctataatttt gatgcaattt
     9241 gtcttgacaa gctactttaa gtaggtcaaa gtttttgttt agtaaggcag atattagtaa
     9301 agcaactcta ctaacattga ataccccatc agaatatggt atttcctttg gtaaaacacc
     9361 tctagctttt tctgttgata acttaaaatt aggtatcatt gcacaaaatt taagttcttc
     9421 aggaattttt ataatatcat aatgtatatt ctcattatca gtaacagata cgatcatatt
     9481 tcctaaaata gcaggagcaa cattatctgg gtgaccttct atttctgaag ctatatttaa
     9541 taattgattt ttatctaaat tagcacctga tagagcattt gcagctataa ctccagctac
     9601 tatacaagag gcactactgc caagacctct acatataggt acttcactgt ggatttttat
     9661 tttgatacca gcaggtattt tttctggttt aactctatca aaaaagtact tcatagatgt
     9721 ataaactaag ttattttcgt ttttgtaagc atcttcacaa ccttctattt caagaccact
     9781 ttcaatttct tcaacataaa atttattgta tatatttaac gcaattccaa gacaatcaaa
     9841 tcctggacct atattagcac ttgttgcagg aactataatc tctaacataa taatcaccta
     9901 tacctttaag aattttttta tagctagttt taattcatct ttttcataaa tatctttatg
     9961 tagtatttct gcatttttaa gatttttaat aggttttgga atatctattt tagaaatact
    10021 tgataatcta tcaattatag caaagtcatc aaggtcttta aaatcacaat ctatagactt
    10081 tagaacatct tcagaaaact tgaatggact tgcagtactt acaataacag tttttgtgtt
    10141 atcagaagtt tcttttttat atttttcata acaattgtat gcaacagcag tatgagtatc
    10201 tatcaaataa ttataatttt taaacacatt gtttatcgtg cttttaactt cttcttcatt
    10261 tgaatattct ccatagaatg aggctaaatt tttttccata tcatcactta ttttgtagac
    10321 tcctttatca ctaaggtctg ataacagctt atttacaata tcagtatttc tatttgatat
    10381 ttcaaacagt aatcgttcta aattacttga aattaatata tccatagaag gagaagtagt
    10441 taatttcaat gttctatttt tatcatatac accagtttta aagaaatcat aaagaacatt
    10501 attatcattt gatgcacata tcaatttgtt tattggaaga cccatttgtt tggcataata
    10561 tccagctaga atattaccaa aattaccagt tgggacaaca aaatttattt tatcatttaa
    10621 atttatctca ctattgctaa ctaatttcat atatgaataa aaatagtatg caacttgtgg
    10681 aagaagtctt cctatattaa ttgaatttgc agaagataat atataattat tatccaatag
    10741 tttttgatta aattttttat cagaaaaaat cttctttaca cctgattgtg catcatcaaa
    10801 atttcctttt ataccaacta catatgtatt ttttccagtt tgagttttca tttgaagttt
    10861 ttgtactggg cttacaccat cttctgggaa gaatacaatt atctttattt tatctatatc
    10921 tttaaatccc tcaagtgcag ctttgcctgt atctccagat gtagcagtaa gaatcacaac
    10981 atctttctct aaattatctt tctttataga tgttttaaga agatgaggca ttatagtaag
    11041 tgccatatct ttaaatgcta aagttggacc gtgatatagt tctaagaaat aagtatcatt
    11101 tactttattt aatggagcta tactactaca atcaaacttt tcatcatatg cattttctat
    11161 acaattttta atttcatctt cagtaaaatc acataaaaat ttactaagta caaaaaatgc
    11221 tatctgagaa tacgttaaat ttactaaatt tataagttca ttttttacat tgggaaattc
    11281 acttggaaca taaagaccac catcacttga tattcctttt attattgctt gtgatgcagt
    11341 tactttttct tcggtgcctc tcgtacttga ataatacatt ttttataatc ctccatatat
    11401 tttaaatttt taattagcta attaaattag atacagaata ttttaaaaac cgaatttagg
    11461 taataaaaaa agaccctcat ccctaaaaaa gggacgagaa tctgtttccc gcggttccac
    11521 ccaaattgat tttaaaaaat ccacttgata ctttaacgca agtaataaaa acgtctattc
    11581 ctactaaaat cattaagata acttcggata gaagctccta ggtagttttc aatccttcta
    11641 attagggaaa catttcagct agtagtttcc ttctctggta atcgattcag atttactctt
    11701 cctaatcaat gcctttcaat attcttaata ttattaatta ttctagttta taaaacagac
    11761 aaagtcaagt attaatataa aaatttggaa aaaatatttt ttaaattgta aaaaaattgt
    11821 a
//
LOCUS       DAEPLF010000100        11805 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEPLF010000100 DAEPLF010000000
VERSION     DAEPLF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.100"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            240..1568
                     /locus_tag="KR314_002261"
     CDS             240..1568
                     /locus_tag="KR314_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0328642.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGDKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGIKR"
     gene            2323..3156
                     /locus_tag="KR314_002262"
     CDS             2323..3156
                     /locus_tag="KR314_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBH0328643.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            3249..4181
                     /locus_tag="KR314_002263"
     CDS             3249..4181
                     /locus_tag="KR314_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0328644.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKEYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            4233..4715
                     /locus_tag="KR314_002264"
     CDS             4233..4715
                     /locus_tag="KR314_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0328645.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            4727..5596
                     /locus_tag="KR314_002265"
     CDS             4727..5596
                     /locus_tag="KR314_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBH0328646.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGVIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKAHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            5639..6394
                     /locus_tag="KR314_002266"
     CDS             5639..6394
                     /locus_tag="KR314_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBH0328647.1"
                     /translation="MTTYKKLKKSIFKISIFSICLLVSLVDINPIFAGSSSNETSNSD
                     TIKVLNAKSLNFKQGNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEDSSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDFPVKDFESINKYQDFYFNKN
                     GNLVISFEEYEVVPGYMGAVEFVIPNKVIKRLR"
     gene            6436..7257
                     /locus_tag="KR314_002267"
     CDS             6436..7257
                     /locus_tag="KR314_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HBH0328648.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANYER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKRETGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            7262..8023
                     /locus_tag="KR314_002268"
     CDS             7262..8023
                     /locus_tag="KR314_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBH0328649.1"
                     /translation="MNTGSKDIDTLNVNFRGIEIKAVLVYKNRKNISIKIDPFGKIIV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLKDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERTVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    8184..8441
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8554..8793
                     /locus_tag="KR314_002269"
     CDS             8554..8793
                     /locus_tag="KR314_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBH0328650.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            9063..10058
                     /gene="ilvC"
                     /locus_tag="KR314_002270"
     CDS             9063..10058
                     /gene="ilvC"
                     /locus_tag="KR314_002270"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBH0328651.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            10111..11799
                     /gene="ilvB"
                     /locus_tag="KR314_002271"
     CDS             10111..11799
                     /gene="ilvB"
                     /locus_tag="KR314_002271"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBH0328652.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWSAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKAGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSNMEELGKVLDVIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
BASE COUNT         4899 a         1157 c         2099 g         3650 t
ORIGIN      
        1 atacaatgta tattatacaa tataaaaaat acgtttattt ataaatatta ttatatttgg
       61 atttttggtt tttataattt ttttatattt aaacataact aaaagtaaaa gattacgaaa
      121 gctaagtaga gtttatttta ttttaataaa taaaaaagct atttttctat atattggcat
      181 aatatatgct tatttaacta tgaaattaaa attaaatagg attaatgagg aggaaaagaa
      241 tgggaagtaa tgaactgaaa aagactcttg gcgtatcagc agcattatca actgttgttg
      301 gttctgttat aggagcgggc gtatttttta aaccacaagc agtctataca ttaactggag
      361 gagctccagg tcttggtata ttggcatggt taatagctgg aatcataact ataacagctg
      421 gtcttacagc tgcagaagta tcagttgcga taccaaaaac tggtggaatg atggtatata
      481 taaaagaaat ttatggagac aaattaggtt ttttaactgg atggatgcaa attgtattat
      541 tttatccagg gatgatggca gcattaggtg ttatatttgg agaacaagca tcagcactaa
      601 ttggtagccc atcactttta cttccaatag caattggaat tatagtaata gttgcagggc
      661 ttaatatgtt aggttcaaaa actggaggag tcatacaaac tgtctctaca atttgtaagt
      721 taataccact tattttaata atggttgttg gatttattaa aggtggtgga aataatccaa
      781 tacttacacc tatggttggt gaaggattaa gcctgggtag tgtactaggt caagttttaa
      841 ttgctatatt atttgctttt gatggatgga tgaatgttgg tactttagct ggagaaatga
      901 agaatcctgg aaaagacctt ccaaaagcta taataggtgg attgtcagtt gttatggctg
      961 tttattttat aataaattta gcgtatcttt gggtattacc agctaatgaa ttagcaaatt
     1021 atgcttctcc tgcatcagca gttgctgaag ttatatttgg ttcaatgggt ggaaaaataa
     1081 tatctgtagg tatattaatt tcagtgtttg gagcattaaa tggattttta ctaactggtt
     1141 caagggtggc atatacttta gctacagata agactttacc aaagtatggt atattttcta
     1201 aattaaattc tgctcaagtt ccagcaaatg caatagcatt agtatctgtt attgcatcta
     1261 tatatgcttt gtcaggtcaa tttaatttat taactgactt agctgtattt gctacttgga
     1321 tattctatgt attaactttt ataggggtta tgaaacttag aaagactcat ccaaatatac
     1381 caagagagta taaagttcca ttatatccta ttgttcctat aatagctata gcaagtggta
     1441 tatttgttgt agttaatcaa ttgtgttttg cagggatgaa aactactatg atatctatag
     1501 gaggtcttgt aataacagct ataggcttac cagtgtatgc ttatatgact agaggaataa
     1561 aaagataaac caagtagttt acaaatgctt tataattaaa ataaaagaga ctgtctcaaa
     1621 atagagataa gtctcttttt tattgttaaa agggtaaaga gatgttttat tttataaaaa
     1681 aagagtgttc tatgaactaa aaagtttatt ttaaggtatt cactttattt ttagtttttg
     1741 aattataaaa ttaaacatct aaaaaattat ttgctttaag atactttatt ttaaagtgtt
     1801 tttttttcaa actaagtgat atctatataa caaatattat acataggttt tatgaaaatt
     1861 aaatctttct gttatataaa atagaatatt gtattatata gaaaatatta cctgatcaat
     1921 atcgtgattt ataaataaga ttagaaaatt ttatttgata acaaaaatta gacaatatga
     1981 atatataatg tatgtattaa aaatgtggca aatagttgtt ttggtattta ttatttgttt
     2041 aatatttcta atttataaaa aaaaaattat aaattttaaa aattataaat gacattttaa
     2101 ctagaattaa ttgtaaaaaa cactatataa tactcaattt tcaacattct atacaattat
     2161 acagaaatta tacagcataa taatcaccga aataacaaat tttaaaaatt aagcaaaact
     2221 ttttgtcgaa gttcaacgtc tatatatatg tagtgtaaat ttttgggata aaatactaca
     2281 aaaatataaa tgtaaaaata tagtaatatt ggaggtcgaa cgatgagaaa taaaaagaaa
     2341 aaaataaata gaaagaagtt gtatttgctt ttaagtgcta ttgtagtgtg tctggctttt
     2401 atagtagttg gtgttcgcgt cagctcttta aatatgaagg aaaatgaaat tagtagaaat
     2461 gcaaaactgt caagttctac tattacagat atagtatcta atacatcaat agaagttagt
     2521 aaagggccaa gtaaaagttc tggaaaaata gcatacataa ctatagatga tggcccatct
     2581 aaatttactg acgagatgat aaaaacttta aataaataca atgttaaagc aacttttttt
     2641 atgatagatg ggaatatgaa ggaatatcca cagcaagtta aaaatataat taaaaatggg
     2701 aacacagcag gttttcatag tgtatcacat gatattcaca agctttatgt tactagtaca
     2761 tcagcaaaag aggaatttga tacaaatgat caaacttttt ataagataac tggtaaacat
     2821 tcaaaagtaa taagaatacc ttatggaagc aaaccatata caccacaagc ctcttatcag
     2881 gctttagttg atgctggata taaaatttgg gactgggact tagatacaga agattggaga
     2941 tctaattctt ctcaaattgt acaaaatgta aaaaatcata ttaagaatag aaaaggtgaa
     3001 gataaagacc aacttgtagt tttaatgcat gagaaaaaac agagtgcaga agctttagat
     3061 tcagttttaa aattcttatc agatgaaggt tatgaatttg cgccagtaga tcaaaaccaa
     3121 ataccaaaaa attattggtt acgtaattta gagtagttaa aatataagaa tattgattga
     3181 tatttttaga aattgagaat aaaaatgagt gaggtgtagg tatattgaat aaaaaacttt
     3241 atataggaat ggtcggaata ttgtctttga tgatggttgg ttgtaataaa agtttagcaa
     3301 aagtaaatga tgtggaaatc acaaaagaac agtacaaaaa aacaaaagca gttttatctg
     3361 ctactaataa ttatataaat gggcagtctt tagatgaatt agaaaaaact ttggataaaa
     3421 aaggtagaaa taaattagaa aatgttataa tatcgtttat ggtggataat gaacttttat
     3481 atcaagaggc taaggataaa ggactaactc caagtaaaag tgaagtagac tcaaaatatc
     3541 aagaactaga agataagatg aatttgaata caagctataa ggaaaaaatg gacaaagcag
     3601 gtgttgataa agaatattta aaacaagaaa tatctagaga tttagcaata gataaaaata
     3661 aaaaggcctt tgaagataga ataaacataa gtgataatga tatggaagcc tactatacaa
     3721 gtcataaaaa agattttaat gtagaagaag taagtgcttc tcagatactt atatcgactt
     3781 tagataaaaa taaaaaagaa gtaagcaaag ataaaaaaga agctttgaag aagaaggcag
     3841 ataatatatt gactaaaatc aaaaatggag aaagttttga aagtttagct aaaaaatatt
     3901 cggatgataa agcaactgga aaaaatggag gacagctagg atattttaca aaggatgata
     3961 aaaatgctga gtttacaaag gaagtgttca aattaaagaa aaatgaagtt tcaaatgtgt
     4021 ttgaaactag ttatggatat catatagtca aagttactga taaaagagaa agacaaaaaa
     4081 gttttaatga gtgtcagagt ttaataagag aatctatttt aaatgaaaag tatattgagc
     4141 atataaaaaa actgaatgaa gatgctaaaa tagacagata gaatttctaa cagctcaatc
     4201 ctagcctgta atcggaggga gattttacag gaatgtttaa gagtttaaag agaacgaaag
     4261 ttgagaaata cattatcgat aataaagact ctttttatag aatagcatat agttatacga
     4321 agaatgaaga agatgcctta gatgtggttc aagaagccat gtataaggca ctatactcag
     4381 tggaaaatat taaagaagta aattatataa agacatggtt ttataaaatt ttagttagaa
     4441 cgagtataga ttttattaga aaaaaccgta aatataataa tatgacagat attgacctta
     4501 tagatgagac tggagagtat gataaatata ctgatttgga tttaagaaga gctttggaag
     4561 aattacctat tgaatacaaa tctattataa tattaagatt ttttgaagat ttaaagattg
     4621 aagaagtagc aataatattg gatgaaaatg tgaatactgt taaaactagg ttatatactg
     4681 ctttaaaaaa actaaaatta aaaattgaag agtagggata aaaagaatga caagcaaaga
     4741 aaggttaaat aagttaaaag atgagtatca taaaattcca atacccaaga aattagatac
     4801 tataataaat catgaaaaaa tagaaaacat atatcgagag aaaaataaaa aagtaaatag
     4861 attaagattt aaagttgcaa tagcctttgc atgtatattt acagtattag taaatataag
     4921 tcctgtattt gcagacaatt tttctaagat acctgtaatt ggagtaatag tagaagtaat
     4981 aactataaaa aattatagtt taaaaagtga aaattatgaa gctgagattg atattcctaa
     5041 aatacgaggt ttaaaagata aaaacttaga acaaagatta aacagtagtt ttatggaaga
     5101 tggaaaaagg ctttatcatc aattccaaga gagaatggag aagatacaat catcaaagaa
     5161 taaaggatat aaatctttaa gtttgagtta tagtgtaaaa aataattcta aaaaatttct
     5221 atcaatagag atgacaaaaa atgagataga ggcttcatca tatgtgagta aggcacatta
     5281 tactattgat aaaaaaagac aaatagtttt gactttgcca atgcttttta aagatgataa
     5341 gtatataaaa gttataagtg ataatataaa agaacaaatg agagaacaaa tgaaaaagga
     5401 tagtacaaaa tcctatttta tagatcaaaa aaaagacttg cctgttgaag attttaaaac
     5461 aattaataag tatcaggatt tttattttaa taaaaatgaa gaccttgtaa tttgttttga
     5521 tgaatatgaa gtagcacctg gttatatggg tgcagttgag tttgtaatac catataaagt
     5581 aataaaagat ttgtaaattg aatagtagtt tatatataca tattttaaga aggacaatat
     5641 gactacatat aaaaagctta aaaaatctat atttaaaatt tctatatttt caatatgctt
     5701 attagtctca ttagtagata taaaccctat atttgcaggt agttcctcta atgagacttc
     5761 aaatagtgat acgataaagg tgttaaatgc taagagtctc aactttaaac aaggcaatta
     5821 tgaagtgaat gtgagtattc ctaaaataaa aggtttaaaa gataaaactt tagagaataa
     5881 attaaacaat gagtttttgc aagatgggaa aaaaatttac agtgaatttc aaaataaaat
     5941 gaaaaaggaa aataataagg gtcatagata cctaaatgta atccataatg tcaaaaataa
     6001 ttctaaggac tttttatcaa tagaaatgac aaaagaagag atagaagatt cctcgtctat
     6061 aactaagaag cattacactg ttgataaaaa tagaggaata ctattaaatt taccaatatt
     6121 ttttaaagat agtagatata ttagagctat aagtgataat ataaaagagc agatgagaaa
     6181 tcaaattaag aaagatgata caaaatctta ttttatagac caagataaag acttcccagt
     6241 gaaagatttt gaatctataa ataagtatca agatttttat tttaataaaa atggaaatct
     6301 tgtgattagt tttgaagaat atgaagtagt acctggttat atgggagcag tagaatttgt
     6361 gataccaaat aaagtaataa aaagactaag atagatatat agaagatttt tagatagact
     6421 ggtaaggaga attatatgaa tatatatgaa caagcaataa gattaataga aaataatgaa
     6481 gatttcgcat ttgctacaat aacgtctcat tcaggttcaa caccacgtga aactggagca
     6541 atgatgattg tgaaaaatga ttctactata tttggttcag taggaggagg tagtgtagaa
     6601 gcagaatgta taaaacatgc aattaatgtt ataaaaaata gagaatcaat gttgtataaa
     6661 tttactctaa acaaatctga tgtggcaaaa ctaggaatga tatgtggagg tactggagaa
     6721 atacaaatag attttataga tagtaaatta aaaagtaata tagaaaaatt caataagaga
     6781 ctaaaggaaa atacaagtaa agcatatata tttggtgcag gtcatatttc aagagatgtt
     6841 gctgtgatac tatctctttt agaatttaga acagtagtaa tagatgatag agaagaattt
     6901 gctaattatg aaaggttccc agaatcagaa gtagtagttt tagattcttt tgaaaatata
     6961 ccagattttc caactgacga aaatagctat ataattattt taacaagagg tcatctatat
     7021 gattcaagtg ctttagaatg ggctcttaaa agagaaactg gttatatagg tatgattgga
     7081 agtagaacaa aaattggatt gacgtacgaa aaactcatga aaaaaggttt taagaaggaa
     7141 gaattatcaa aagtacatgc accaataggc ataaaattga atgctcaaac accagctgaa
     7201 atagctgtat gtatagctgc tgaacttata aattgtagag ctaataagga aaaataaact
     7261 aatgaacaca gggagtaaag atattgatac tttaaatgtt aattttagag ggattgaaat
     7321 aaaagcagtt ttagtatata aaaatagaaa aaatatttct ataaaaatag atccatttgg
     7381 caaaataatt gtaatgagtc ctcctaaaat atccaaaaaa ataataaaag atataattat
     7441 tgaaaaagga gactggatac ttaaaaagtc agaaaaatat aaaggaagag aagaagtata
     7501 caaacaaaga atgtttataa ctggcgaaaa atttctttat ttaggtgaag agtaccatct
     7561 agttataaaa gaactgttga aagattctgt caaaaaaagt aactgtagaa taactattaa
     7621 cgaatatcaa atagttgttc agacaaatga tacaagtaca gattttatta aaaaatcttt
     7681 aaaatcttgg tataagatgg aaagtgaaag aacagtatta gagagaatag attttcttaa
     7741 attaaattgt gaaataatga agcaattagt tccagcaagt gttaaggtaa aagaacaaaa
     7801 taagagatgg ggaagttgta cagctcaaaa aaatatttat ataaactcaa aaatatctat
     7861 ggcaagatta gatgtaattg attatgtaat aatacatgag tttagccatt tagtacatat
     7921 gaatcactct aaaaaattct atgatttagt aaaatccatt atgcccgatt ataaagataa
     7981 agaaaattgg ctaaaaataa atggatataa aataataatc tgaaaacgat tattccaaaa
     8041 tacaggcgta aagtataaaa gaagtattat tgtgaaaaaa ataaataatc tgaaaatttt
     8101 aaaaaaagtc attgacattg taaatgatag ggaatataat ctatattaac aacaaaacag
     8161 aattaaaata taaaattaaa aaattaaaag caatgacaga gacaacaata ctaatagtgt
     8221 tatccacaga gaaccaatgt tgctgagaat tggtgttaac agtagtataa tgatcactct
     8281 ggagcttttg tactgaatat ttttagtaag tacaaacgta tgtcaacgtt acatgactaa
     8341 ggttaaggta attaattacc cgacctgaaa aaagaggggt agatgtattt tttatatcta
     8401 caaataaggt ggtaccgcgg aatataactt tcgtccttat aagtaatatt tatattgctt
     8461 ataaagatga aagttttttt attttaagcg atataaataa atttaatcca tataaaatta
     8521 ataaaaaaat aataaaaata aggagggatt tttatggaaa atactgtata tgctaaatta
     8581 aaccaaggaa tagattcttt tttaagagtg gctatgactc taagaagaag agaggttgat
     8641 atacaatcaa taagtatgac tgtagataat atgaataatt caggtataaa attaatagta
     8701 aatgaagaaa agacatcttt agattctgtc ttaaaccata tgaaaaaact acatgatata
     8761 agagagataa ctgttgaaag aatgagacat taattaaata tattttataa agctaaagat
     8821 aaatattaaa taagaaaaat cttagattaa aatataagtg tcattgaatt tatcagttta
     8881 tcacagttaa taaatttatt agtttgttgt agctaataaa aactgaaaat tctaaaatta
     8941 taattgtaag attatataaa tttagaattt aaatctgttg aataatatta aaacaatgat
     9001 aatgagtatg aatttctaat aaaaataaat atcataaaaa taagaaaaag tattgggagg
     9061 aaatgaaaat ggcaagaatg tattatgaaa aagatgtgga tttagaggtt ttaaaaaata
     9121 aaaaggttgc agttttaggt tatggaagtc aaggacatgc acatgcacaa aatcttagag
     9181 ataacggtgt acatgtagtg ataggtcttt atgacggaag taaatcagca caaaaagcaa
     9241 aagaagatgg atttgaagtt aagagtgtag cagaagctac aaaagaaagt gatttgacaa
     9301 tgatgttaat gcctgatgaa aaacaaaaga aagtttatga agagagtgta aaggataact
     9361 taaaagaagg tcagacatta gcttttgctc atggattcaa tatacattat aatcaagtac
     9421 aaccaccaga atttgttgat gttgtaatgg tagctcctaa aggacctggt cacttagtta
     9481 gaaatgtatt tacaaaggga agtggagttc ctgcactatt tgcagtatat caagactaca
     9541 ctaaaaaagc tacagagaca gttttagcat atgcaaaagg gataggagca acaagagcag
     9601 gtgttttaga aactactttt aaagaagaaa cagaaacaga tttatttgga gaacaatcag
     9661 tactttgtgg tggaataagt gaacttataa aattgggata taaaacgctt gtagatgcag
     9721 gttatcaaaa agaagtagca tactttgaat gtttacatga aatgaaacta atagtcgacc
     9781 ttatatatga aggtggattt gaaagaatga gatatagtat aagtgacaca gcagaatatg
     9841 gagattatgt atctggtaaa agggtaataa ctgatgctgc aaaacaaggt atgcaaaatg
     9901 ttttagagga tatacaaaat ggaaaatttg caaaagcgtg gattaaagaa aatgaagaag
     9961 gacgtgaaaa cttcctaaaa actagagaag aagaatataa tacagaaatt gccgaagttg
    10021 gtagaaatct aagaagtatg atgtcattct taaaataaat atatattaaa tggattagtt
    10081 tgagggaaag agtggtgtat agtagtgcgt atgaatggag caaaggtaat tttagaatgt
    10141 ttaaagaaag agggaattga cacaatattt ggatatcctg gtggagccgt gatacctctt
    10201 tatgatgctc tatacgatta ttcagatgat tttaaacata taaggacatc ccatgaacaa
    10261 ggtttagttc atgctgcaga tgggtacgca agaagtacta atacagtagg agtttgtttt
    10321 acaacatctg gtccaggagc aacaaatgcc ataactggaa tagcaacagc ttttatggat
    10381 tcttcaccta tggttgtaat ttcagggcag gtgccaacta gtttattagg aaaagattct
    10441 tttcaagaaa ttgacataac tggagcaaca ttatctatga caaaacataa ttatttagtt
    10501 agaaacacta aagaattagt tcctacaata aaagaagctt ttagagttgc aaattcaggt
    10561 agaaaaggac ctgtacttgt agatgttcca aaagatttat ttttagcaga aatggatttt
    10621 agtggtgagg actatgattt atgtcaaata gatgattata tggattataa gagtgatttt
    10681 gatctagatg atgaaactaa tattaagctt ttaaatgaag caatagatat cataaaagaa
    10741 tctaaaaagc ctgtaatata tgcaggtgga ggtgttaaat cttcagacag tgaggaaatt
    10801 cttgaaaaat ttgcaactaa gatagataca cccgtactaa atacacttat ggggcttgga
    10861 aatatagata gaaagaatga actatctctt ggaatggttg gaatgcatgg aagtagagaa
    10921 agtaatttag cactttctaa ttcagattta gtaatagcga taggtgcgag atttagtgat
    10981 agagtgataa gtaaaagttc tgaatttgct aaaaatgcaa aaataataca catagacata
    11041 gacccatctg aaataagtaa aaatatagaa tctaatgtat ctttggttgg agatgtaaag
    11101 ctagtcctta gtttattaat tgaaagggtt gaaagtaaga ataatagcaa ttggaaagaa
    11161 gagataaaga catttagaaa gagtgagggt gtacaaacat atgaatttca tccacaaaat
    11221 atacttaaaa aaataaatga aaaatatgaa acattaaaga aacccactgt ggtagtaact
    11281 gatgtcggtc aacatcaaat gtggtctgct aaatactgga attttaaagg taataaaagt
    11341 tttataacat cagcagggtt aggaactatg ggatttggtt taggtgcagc tattggaact
    11401 aaagctggta atgtagataa aaatgtggtt ttagtaactg gtgatggaag ttttagaatg
    11461 aactgtaatg aactagctac agttgcaaat tataatgtac ctatgcttat tttactactc
    11521 aataatagaa cattaggaat ggtaagacaa tggcagaagt tattttccaa tcaaagatac
    11581 tctcaaactg atattaatga aaatgtggat tatgttaaac tcgtaaatgc atataatatt
    11641 gatggatata aggtatccaa tatggaagaa ttaggaaagg ttctagatgt gatagatttt
    11701 aataaacctg tatttttaca atgtgatata gataaggatt atgatgttta tcctattgta
    11761 gcacctaatg atgcattgga aaatttgata tgtaactaaa actta
//
LOCUS       DAEPLF010000101        11776 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEPLF010000101 DAEPLF010000000
VERSION     DAEPLF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.101"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(384..1787)
                     /locus_tag="KR314_002272"
     CDS             complement(384..1787)
                     /locus_tag="KR314_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0328653.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            2664..3173
                     /locus_tag="KR314_002273"
     CDS             2664..3173
                     /locus_tag="KR314_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBH0328654.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            3201..3395
                     /gene="rpmI"
                     /locus_tag="KR314_002274"
     CDS             3201..3395
                     /gene="rpmI"
                     /locus_tag="KR314_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBH0328655.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            3451..3807
                     /gene="rplT"
                     /locus_tag="KR314_002275"
     CDS             3451..3807
                     /gene="rplT"
                     /locus_tag="KR314_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBH0328656.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            4117..4662
                     /locus_tag="KR314_002276"
     CDS             4117..4662
                     /locus_tag="KR314_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328657.1"
                     /translation="MNEKLMDCFVNPIKCKLLLEVFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKEGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            4649..4822
                     /locus_tag="KR314_002277"
     CDS             4649..4822
                     /locus_tag="KR314_002277"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328658.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLHGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            5098..7023
                     /locus_tag="KR314_002278"
     CDS             5098..7023
                     /locus_tag="KR314_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0328659.1"
                     /translation="MKIKFVIMPLVIFLIVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFISNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            7424..9328
                     /locus_tag="KR314_002279"
     CDS             7424..9328
                     /locus_tag="KR314_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0328660.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFKQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEGKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
     gene            9716..10495
                     /locus_tag="KR314_002280"
     CDS             9716..10495
                     /locus_tag="KR314_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBH0328661.1"
                     /translation="MNYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKVFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVVLGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(10805..11626)
                     /locus_tag="KR314_002281"
     CDS             complement(10805..11626)
                     /locus_tag="KR314_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HBH0328662.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDITVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLEDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
BASE COUNT         4649 a         1339 c         1967 g         3821 t
ORIGIN      
        1 aagtgataga tacctaatta agttgtagat atctgataag aaatttttaa gttttcaatc
       61 gttctaaatt gtgagtataa aaatctacta aatttagtta tgtaaaatat aaattataag
      121 tgtagaaact aggctactag cttttatacc acttcaaaca ttactggaag tagttaagtt
      181 tacagcagtt tagttttatg tagatataat attctgtaaa tagacaaaat taaaatatgt
      241 aattaaaata tgtaagtgtc tcaaaatgaa atttttaatt tatgaggcac ttttttgtat
      301 aaaatcaaat atatttttat aactttaaca ataaaaagag gcttatctct attttgagac
      361 agcctcaaat ttttaaaact atattagttt acagcttctt ttcctatcaa atcttttaac
      421 tcttcatgag tcatagtttc tttttcaaat aagaatttag aaacagaatg taaatcttca
      481 agattatcct ttaacatctc taaagtctct ttataacaaa tttcaactat acgatttgct
      541 tccttttgaa ccttatcaca taagaaagtc ttatcatttc catcaatagt cataaatcct
      601 aagttactca taccatattc acaaaccatt tggttagcta tttcagtgac tttctttaag
      661 tcgtcctttg caccagtaga tttatgatta aatattaatt cttctgaagc ctttccagca
      721 agtaatattt ttatttttcc aatcaactca tccttagtat aaatatatct atcttcatca
      781 ggtaattgaa gaacataacc taatgcttgt cctctaggaa ctatagatat tttttgtata
      841 ggacaaattc ctactatatc acttacaaga gcatgtccag cttcatgata tgctactatt
      901 tttttctctt tttcaaccaa tgcagagttt ttagattcta gccctgcaat aactctttca
      961 agtgccttgt caaaatgttc tgatgtaata cactcactgt catctctaac tgcaaatata
     1021 gctgcttcat ttgctatatt tgaaagatga gccccattga aaccatgagt ttttttagct
     1081 aaaagttcta gatttacaga tttgtctata ggtttgtcat ctgtatgtac ctttagaatt
     1141 tcaaatctag tgtgatagtt tggtgcacct atatgaatgt gtctgtcaaa tcttcctggt
     1201 ctaagtaaag cttcatctaa taagtcaagt ctattggtag cacctattat taatacattg
     1261 gaatctttat taaatccatc catttcaact aaaagttggt ttagtgtttg gtctttttcg
     1321 ttgttacttt caagatgtct ctttgctcct acagcatcta tttcgtctat aaatattata
     1381 cttggagctt cttttctagc tttttcaaat agagttctaa ctctttttgc tcccacacct
     1441 acatattttt caacgaactc tgaaccagta acattgaaaa atgatgagtt tgtttcacca
     1501 gctacagcag atgcaagtag ggttttacct gttcctggag gaccataaaa taatactcct
     1561 ttaggtattt ttgcacccat tttttgatat ttttgtggtg acttcataaa atcgactatt
     1621 tcaaatagtt cttcttttac ttcgtctaat ccagcaacgt ctctaaatgt agtctttggt
     1681 tttgtagaaa gtgaatcatc tgtttgatta tctctttcca tttttgtctg tgcaaagaca
     1741 ggagctggat tgttgatttt cttaaaaaag ttgtttagca ttttcatatt cacacctcag
     1801 gtaattaatt attgttaatc aattttatat tttccatatt tactattttt tattatttat
     1861 ttttcatgga aaaaatatac atgttatcta aataaaaata aatttgtaag atgtatgaaa
     1921 aaatgaattt atgtatatag agatttctac ttatgtattg aataaagtat tacaaattat
     1981 aaattatgta ttgattatat attgagtaaa gtattataaa gcatgtattg attaagtatt
     2041 tattaagtat tgaataaaat attacaaatt tagttgaaat gaaaatgata aaaaatgttg
     2101 tgctatattt tacaaatcat ataaatagag aaattgttgt agcacaaagt aataggtagt
     2161 atattagaat ataatatagg atttgttgtg tagtatagta gcatattaga atataatata
     2221 ggatttgtta tgtagtatag tagcatatta gaatataata taggatttgt tatgtatata
     2281 gtagcatatt agaatataat gtaggatttg ttatgtagaa tatatagagt catgtagaat
     2341 ataaatatat aaaaggattt tgggtataaa tttaaaaatt aatagcaaaa tgagtagtat
     2401 agacatataa ttagtagtag gaagtaatta aatttttcct aaacaaaaaa aatagttgca
     2461 aataatttga ttaagtgata tagtaaataa ggtaagaata aaacaaagaa gaagtccgct
     2521 tctcacctta cgccggaggt tagtaaggta aatataagtt aaagcttaat tttaatttaa
     2581 gtttgtttaa atttggtaac ttatattgcg gatgattacg tcatccgctt tttattttat
     2641 aaaaattttg gaggtgtcct acaattagca aagaattagc aatcaatgaa caaataaaag
     2701 ataaggaaat aagagtcctt tcaccaacag gtgaacaact tggagtaatg cctactaaag
     2761 aagctcaagc aatggctaat agcaaaaact tagatttggt tcaaatctcg ccgaatgcga
     2821 atccaccagt atgtaagata atggattatg gaaagtttag atatgagcaa gcgagaaaag
     2881 agaaagaagc taagaaaaaa caaaaaacta tagttgtaaa agaagtaaga cttagaccag
     2941 gtattgaaca aaatgattta aatacgaaag ctaataatgc tataaagttc cttaaaaaag
     3001 gggataaagt caaagttgaa cttagattta gaggaagaga attaggacat aaagacatag
     3061 gtaaagaagt tatgcttaag tttttagata taataaaaga gtttggagaa ccaacaaaag
     3121 cccctgcatt tgagggtaat aatatggttg taattataga tccaaaaaaa tagatgatta
     3181 actgagagga ggaatttgtc atgcctaaaa tgaaaactca tagaggtgca gcaaagagat
     3241 taaagaaaac aggaactgga aagttaaaga gagctaaagc ttttaaaaaa catatattaa
     3301 ctaaaaaatc tgcaaaaact aaaatgaact taagaaaatc aactttagta agtgatggag
     3361 atgccaagag aatagcacaa ttattaccat actaagatac agattgtaaa taataactat
     3421 agaagaggga tataaaagga ggtcataacg atggcaagag ttaaaaaagc tatgaacgct
     3481 cgtaaaaagc ataagaaaat attaaaactt gcaaaaggat tcagaggatc tagaagcaaa
     3541 ttatatagac cagcaaatac attcgtaatg aaagcattaa aaaatgctta tataggaaga
     3601 aagttaaaga agagagactt cagaaaactt tggatacaaa gaataaatgc agctgctaga
     3661 atgaatggaa tatcttattc aagattaatg aatggattaa aattatctgg tattgaagtt
     3721 aacagaaaaa tgttatctga aatggctata caagacccag aaggatttgc aaaattagca
     3781 gaagttgcta aagcaaaatt agcttagtat tgaatcatga gatgtaaagt ctttgagtta
     3841 tataaataat tcaaggactt ttttattttt tgaaaaatac ttattagtgt catgtacaac
     3901 caattttata atgataaata aaataagcaa tacaatagaa ttttatgaga tttattataa
     3961 gaattgttac aattatttat catagctata aaaagtcata gtaaaaatga tattgatgta
     4021 tgtttatata tgtataaagc tttcaatcaa tcttgaatta atattatcaa catgataata
     4081 ttatcttatt aataatataa ataaggggga gataatatga atgagaaact aatggattgt
     4141 tttgtaaacc caattaaatg caagttattg cttgaagttt tttcaaataa tcgaactaca
     4201 gctaaacaat taggagaaaa atttcaagat atatcaaagg caacacttta tcgttacctt
     4261 aataaaatgt tgaaagatga tattataaaa gtggttgaag aaaataaaat aaggggaact
     4321 gttgaaaaag tatatgctat gaagttagat atagctgatg atgtgaacaa aattatagat
     4381 gagaactctg gagaagaata tgctcaactg tttactcaat atatgatggg atttttaaga
     4441 gaattttatg aatacgctga aagaaaggaa aaggatatcc taaaagaggg ttcaggattt
     4501 tctgtctgtc cagtatatgc tagtattgat gaaatagttg aagtgagtaa aaaaattgct
     4561 agtatactag aacctctaag aaataactcg cacgaagaag gcagaagaat gcattctata
     4621 ggagtgataa tcactccacc taaagaggat gaagggaaat aaatctgatt tacatgaggt
     4681 agagccaatt tacacggaaa atcgttttat gatttacata tgtatccaga gcaaagtatc
     4741 tctgcatgga ttgtacttca ttgtaccatt tcatccaatg acttggagtg gaacaatgaa
     4801 gttttttaat ttttcatatt aatgcctaaa gaaatgaact ttagaccaca tatgcaatta
     4861 ggctacatta tacaattaga ccagatatac agaataaaaa ttagtatttt tatagcagat
     4921 aaattgactg ttattcttct taaaaatcat ctattagtta ttgttagcat attgaaagaa
     4981 attatagtaa tccattgaat aaatgaatat tgcccataat catataaact gctatagttt
     5041 aaaataaagc aggacgttaa attttatagt aatcttaaaa tttaagaggg ggattttatg
     5101 aaaataaaat ttgttattat gccattggtg atatttttaa tagtattatt tgtggggtgt
     5161 caaagtacaa aagatacaga agttaaagca aaagatacag aaattaaaat attaggtaca
     5221 ggtgatttgc attcaatttt gacagactct atggtttcat atgtaaatga ggaaagggaa
     5281 aagaataaaa atctgcttat ggtagatgcg ggagatttct atggtacaca aagtagagaa
     5341 atgtgggaat ggtcttctgg aaaaaagtta attaatatta aaagaaatgg tagagcagag
     5401 tatgatgaca taattaaaag tagtaaagat gaagtaccta tagttaaaga tatggcaaag
     5461 ttgaaatatg atgctgtagt tttgggagat aatgaattta tttcaaatga taagcaaagt
     5521 cttgataaac tagtttctga ttttaaaaat aataatatgc cattggtatc agcaaatata
     5581 tatgaacaat ctggagaaaa ttatgtacaa ccatatgtaa tgaaaaatat taagacagat
     5641 gagggcaatg taaaagttgg aatactaggt cttactataa aagaagtagg ggaaaccctt
     5701 ggtcttgagg attttgaaaa ggataaaaaa gctagagagc ttggagaaca agctgattac
     5761 aaaggaaaat tatatgcaaa tgatttagta gaagatgcaa agaaatggat aaaagttatg
     5821 gaaaaagaaa gtccagacat aatagttgca gttgtacact caggggaaga acctaaaaca
     5881 gatagaaatc caggaaatag aataaaagag ttagcaacta cagtggatgg aatagatgca
     5941 attgtagctg gtcatactca tgaaaaaata gatgaacaca aatataaaaa taaatctgga
     6001 gatgaagtaa ttgttacaca accaggttgg catggagata gagtatcaga gattaatttc
     6061 aaattaaata aagagaatgg aaagtgggac ataaaggaaa agtctagtaa attaaaaata
     6121 attgataaag aagtaagtat agttgctact gcagatttac attcacattt ctctgataaa
     6181 ttaacagttg accttgttaa tgaacgtagt aacccaattg aacctgttgt agtagatgca
     6241 ggcgacttct tagaccctca aacagatgaa atgactcaat ggtataaaga gtggaaaagt
     6301 ataagagata ataatagtga taaaacgatt agtagatgcc caatggtcat ggatatgaat
     6361 cagggaatgt atgatgcagt agtacttgga aatcatgaat ttgtatctga aaaagatgaa
     6421 ttttggtcag atgaacgcct tttagatgct gttatagagg attttgaaca ggtggcaatt
     6481 tctgtgttat cagcaaatat atataggcaa tctggtaaaa attatgttaa accatatatt
     6541 ataaaagatg tggagacgag tgaaggtaat gtaaaagtag gaatattggg acttactata
     6601 aaagaagtag gtaaaggtct taaaaatgta aacttacaag agcaatttca atataaggat
     6661 aaattgtatg caaatgattt agtggaagat gcaaagaaat gggtaaaagt tatgaaaaag
     6721 aaaaaaccag atataatagt tgcagttgta cattcaggag aagaaccaaa aaatccaaaa
     6781 cacccaggaa atagaataaa agagctagca actacagtag atggtattga tgcaattgtt
     6841 gctagtcata ctcatgaaaa aatagatgag catgaatata aaaataagtc tggagaaaaa
     6901 gttatagtga cacaaccagg tgagcatggt aaatattatt ctaaaattac ctttaaagta
     6961 aataaagaaa agggttcgtg gattattaat gataaattca gtaaaacaat tgaggttgaa
     7021 taactttata aaatttatag ataaagtaaa ttatctgata ttgacatgat atataagata
     7081 aagtttctaa tattagtata atgcataatg tgaaatattt taataataag agaataaaca
     7141 agacaaataa aaatatttta acatgtaaga tatagtatga actattatac taatgcttac
     7201 tttataattt gaacatgtta gaataagtct tatttgaaaa ttttagtgta ttagagtaag
     7261 tattatttta ccagtaaaaa atattgagaa ctatatgtag atttgcttaa aaaggaattt
     7321 acatatagtt cttttcgtat aataaatagt acaatcttat atggtaaaga taaagtgtga
     7381 tattaaattt tattatcatc caaaatctaa ggaggagtct cttatgaaaa taaaatttat
     7441 tatagtagca tcaataatat ttttaatagt attacttgtg gggtgtcaaa gtactaaaga
     7501 cacagaaatc aatgtactcg ccacaagtga tttacattca attattccag ataatttaat
     7561 ttcctatgta gaagaggaga gaaaatatga tgagaatcta cttatggtag atgcaggaga
     7621 tttttttgac atgcaaagtg gtgagatgaa taagtggttt actggtcaaa aattagtaag
     7681 atttaaagat gggataccag agtacaaaaa aatagctgaa ccacgtgaag gagaagttcc
     7741 attggctaaa aaaatggcaa agctgaagta tgatgcagtt acatttggga atcatgaatt
     7801 tgtatctaat gacaagcaga gccttgataa attggtttct gattttaaaa ataacaatat
     7861 accattagta tcagcaaacg tatatgaaca atctggagaa aactacgtaa agccatatgt
     7921 aatgaaaaat ataaatactg acaagggaac tgtaaaggtt ggtatacttg gtctgactat
     7981 aaaagaagta ggggaacgtt caaggtttaa aagttttaaa caagataaaa aggctagaga
     8041 acttggggaa caacctcaat acaaagggaa attatatgca aatgatttag tagaagatgc
     8101 aaataagtgg gtaaaggtta tggaaaaaaa agaaaagcca gatataatag ttgccattgt
     8161 acattcaggt gaagagccaa aaaatcctaa aaatccagga aatagaataa aagaactagc
     8221 aacaactgta gagggaatag atgctattgt agcaggtcat acacatgaaa aaatagaaca
     8281 gcatacatat aaaaataatt ctggtgaaga agtaatagta acacagcctg gagctcatgg
     8341 taatagtata tcaaagatta attttaagtt ggataagaag aatgataagt ggtttataaa
     8401 agacaaatat agtgaattaa aggtttttgg aaaggaagta aatataatta caacttctgg
     8461 tctgaatcca aaattttcag atgaaatggc agttaatttg tttaatgaaa gagatagaga
     8521 tgttcaacct atctttatag atactggaga ttttttaaat actaatacaa aagaaatgac
     8581 taagtgggct aaagagtggc aatacaatga aaataaaggc atctataaaa aaataaatga
     8641 atggccaatt gtatatcttg gttatgatgc tgtagtactt ggtagccatg aatttacaga
     8701 ggataaagag acttttgagg gaaaaaaatc tacattggat tatattatag aaaactttga
     8761 agaaatgaag ataccagtat tatcagctaa tatatatgat gaatctggag aaaattatgt
     8821 aaaaccttat ataattcata atgtagaaac tagggaagga aatataaagg ttggagtatt
     8881 aggtcttact actactgata aggatgaaga agacttaagt agctcaagct taagcaaaga
     8941 ctcaaagact tcgaaaattc ctgaagaaac aggaaagtta tatactaata atctagtaaa
     9001 agatgcaagc gaatgggtaa aagttatgca aaaggaaagt cctgatgtga ttgttgcagt
     9061 tgtacattca gataataata gtactctaaa aaatccaagt agtgaagtca aaaaacttgc
     9121 aatgtcagta gatggaatag atgctattgt tgtaggtcat acgaaaaata aggtagaaga
     9181 acatatttac aaaaataagt ctggagatga ggttatagta acacaatctg gagaagattg
     9241 ttattcaaaa ataagtcttg aactaagaaa agaaaatggc aagtggaata tcataaacaa
     9301 atatagtaaa gcaataaaaa tgaagtgaat ttattgatat ataaaccatt attaacactg
     9361 atttttataa tataataaat actgattcta caatataatt aacactactt ttttaaatat
     9421 aatcaatact gattctacaa tattattaat actacttttt ataatataat caatactact
     9481 ttttacaata taatcaatac tgattctata atataattaa cactactttt taaatataat
     9541 caatactgat tctataatat taacactatt ttttatgcta tataacaatt attgaaaatg
     9601 atataaatat aatatctcaa atgatatgaa tataacatct gaatagaggg tgttttattt
     9661 gtaaaaaata agtttacagt ttgagcgtat aaacttatgg taaaggagaa taattatgaa
     9721 ttataagaag attgcaatta taacgggtgc taatagtggt tttggtaaag agtttctaaa
     9781 gttattaata aatgaagaag aaataacaga aatatgggca gttgcaagaa acaaggcgag
     9841 attaaatcaa ttagtagatg aatttggaag taaggtaaaa gttttttcaa aagatttatc
     9901 aaaaatagaa gaagtaaaag aaattggtac atttttaagt aaagaaaatg tatgtataaa
     9961 ataccttata aacaatgctg gttttgcaaa attctgttca tacaatgatt taagtataga
    10021 tgaatctatt aatatgattg atttaaatat tagtgctgtt gtagtattgg gacttatatg
    10081 tattccatat atgcaaaagg gaagccatat tatgaatatt tcttcacaag catcattcca
    10141 gccattgcca tatcaaaata tttatagttc aactaaagcg tttataagaa attatacaag
    10201 agcattaaat gttgaactta agagtaaagg gataaatgca attgctgtat gtccaggttg
    10261 gatgagtaca aatctattta agagaggtat agttgtagct gaaaaaggga caaaaaattt
    10321 cccaggcata gtgtctccag acgttgttgc caaaaaagca ttgaaggatg caaagaaaaa
    10381 taaggatatt tctgtgtatg gtataaatac aaaatccagt catttattag caaaactatt
    10441 gccacaaaaa atgatgatga aggtttggtt aatgcaacaa catattaagg aataagagtt
    10501 tcggtaatta agtatcagtt ggagattcaa tctattatat attataagaa gttttgttga
    10561 gcaaataata attgtgacaa aacggcttat ctagtgccga taagccgtct ataatgtata
    10621 aaaaatatat ttttgatttt actttattta gatattgaag tattaaaata tgtaggactt
    10681 gaaattttat aaagataaat actatgatat tttgtaaaaa ctgagatatg ttgatattac
    10741 atagtttaac tatactgtta aattgaagtt ttcttatttt gcattatgct tattacttaa
    10801 tgatttagac tataatttcc ataccatcat atgcaaaata aatgttatca agtttctttt
    10861 ctaattcact gtaataacca tatgattttc cccacatttc gtcaatatgt gtaatgataa
    10921 tctttttaat tctataatag tttttaattt caacaatctc atctaaagta aatatttcat
    10981 ctttaaatgg tgtgtcttca agttttgtac catcatttaa tataccatca tcagaaacta
    11041 gacttattat taaaacatca gcatcaaagt acatatcact atgagtaaat ggcttgggat
    11101 tacagcaggc atatatgact tttttttcat tctctttgat aacatagaag gttatattcc
    11161 tgtctgggtt attgttaatc aaatcaatat caaaattatt tatctttaca tgcgaagttt
    11221 cattaataga aatgcaattt ataacatcac cataatattt aagacaatcg agattcgttc
    11281 ggtttatatc ttcaattact tcaggaagag cataaaagcc aattggatta ttttctcctg
    11341 tgataaagtc acatcctatt ttttcgacaa ttctaatacc cttaacatgg tctgggtcat
    11401 ggtgagaaat tgaaatatgt tttacttccg atattttttg tctgttacaa gctactgtaa
    11461 tatcttcagg tgtgtctatc agcattttta tatcatcaat atataaactt ggtcctaatc
    11521 gttcatatct tccgcctttt tgcctggctt cttcacatat tttgcattta caaaatgaac
    11581 ttggaataat tgacataccg ccactaccaa caatttttaa tttcaaagtt attacaccac
    11641 cttctaaaaa tattagatta tataaataat ctacaatcac aactgattaa caaaattatg
    11701 agttaataaa ttatatcaca tgtaaatatg aaagaatagt atgaaatttt tataagatta
    11761 tattaaatat ttctca
//
LOCUS       DAEPLF010000102        11730 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEPLF010000102 DAEPLF010000000
VERSION     DAEPLF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.102"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(10..633)
                     /gene="ytaF"
                     /locus_tag="KR314_002282"
     CDS             complement(10..633)
                     /gene="ytaF"
                     /locus_tag="KR314_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBH0328663.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLELNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            818..1462
                     /locus_tag="KR314_002283"
     CDS             818..1462
                     /locus_tag="KR314_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0328664.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQRPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            1676..2734
                     /locus_tag="KR314_002284"
     CDS             1676..2734
                     /locus_tag="KR314_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBH0328665.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCIFLENCDFNRTIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            2838..4022
                     /locus_tag="KR314_002285"
     CDS             2838..4022
                     /locus_tag="KR314_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH0328666.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADGKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNEKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGVIKYYTSSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            4229..4942
                     /gene="rpsB"
                     /locus_tag="KR314_002286"
     CDS             4229..4942
                     /gene="rpsB"
                     /locus_tag="KR314_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBH0328667.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            5021..5932
                     /locus_tag="KR314_002287"
     CDS             5021..5932
                     /locus_tag="KR314_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBH0328668.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            6017..6721
                     /locus_tag="KR314_002288"
     CDS             6017..6721
                     /locus_tag="KR314_002288"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBH0328669.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            6747..7304
                     /gene="frr"
                     /locus_tag="KR314_002289"
     CDS             6747..7304
                     /gene="frr"
                     /locus_tag="KR314_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBH0328670.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            7307..7510
                     /locus_tag="KR314_002290"
     CDS             7307..7510
                     /locus_tag="KR314_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328671.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            7539..8273
                     /locus_tag="KR314_002291"
     CDS             7539..8273
                     /locus_tag="KR314_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBH0328672.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            8286..9065
                     /locus_tag="KR314_002292"
     CDS             8286..9065
                     /locus_tag="KR314_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBH0328673.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
     gene            9889..11700
                     /locus_tag="KR314_002293"
     CDS             9889..11700
                     /locus_tag="KR314_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH0328674.1"
                     /translation="MKKKYDFLIYSAIWLVIIIFFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILYNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
BASE COUNT         4819 a         1195 c         2029 g         3687 t
ORIGIN      
        1 gctttcttat taacatatct tcataaatcc aatgactaac aagattaaac cacttaacca
       61 agataagttt aaatttacgt ttttaacaaa catatttcct agtaaacaac caagtaatac
      121 tgcaattata tttgatatta aagaaaatag aataacttgt atataattca cacctacaag
      181 gccactacca aaaccgatag ctaatccatc taaacttaaa gcaagtgcaa gtgtaaatgc
      241 ttcagcagat ttaagcactt tagatttact tgtatctaag acatcaggtt cgatacttac
      301 attaaaattt aattcaaggt ctaacatatt aaaattataa ttctttgagt gtatagattt
      361 tttcttaaaa taagatttaa aaaaactttc aattagtcta gcacttccta ataaaaataa
      421 aataaagaaa catattaaca ttgttatatt ttgaggtaaa aaatctctaa ctattgtacc
      481 tgcaaaaagc gatattgcta aaatagctga acaaactacg ctgataatta tatttgaata
      541 taagggaact ttaactttat ttactccata tccaaagctt gtcacaaagg catctataca
      601 aagagaagta actaataaaa ttgattcaat cacagtagat taacatcctt tctcttaaat
      661 tttgtactta tattagttat agtattgtaa aaatggataa attgttacag ataatccaat
      721 aaattgttga taaaatttca acatcataca acacctaaat aacaaaagaa tcatatcata
      781 taatataaaa ccaatcataa taaagcaggt gaaaataatg gaacaacata gtaaggttgg
      841 gaatttcggt gatttgttga aagttttatt agatgaaaaa gaattatcaa tgggagaact
      901 aagtaaaaaa agtggaattg ataaatctac tatatctcgt atagctaata ataaacaaag
      961 gccaaatata aatcatttag aaaagatggc aatacattta aatattaacc tagaagaact
     1021 gttgaaagct tcaggatatg aatttagaaa cagcaataat caaatattta atgcagatag
     1081 tgattttagt aactttgatg atattcttgg atttgcaaat ttaatcaatg ataagaactt
     1141 taatggaaat attgaaaaag agttaagtaa gtgtaagtta tatgtccaaa cagatgaagg
     1201 taaaaaatta ctttttgata attttaataa aaaaatagat tctattgaaa aacaaggtca
     1261 atttacagat agattaagac aaatgtatgc tgatttttgt acagatggac ttacaataaa
     1321 gaaatattta ttgattggaa gtatgctact ttactttgtt atatcaacag atgtgattcc
     1381 agacttcgtg ttcccaatag gatttatgga tgatttagta gcattaaata tagttacaaa
     1441 attacttaaa aatgataagt aagttttaga ataatattta atatataaat aaatcaaagt
     1501 atataaaatt tattattttt tatatgaaac aaattaaaag cacaatgaag tagattaaaa
     1561 atttaaaact aagaatctaa tatatatttt gtaactattt aagtatataa aataaaataa
     1621 tatccaaact aatacaaaat agtaataatt gttttgtact ggaggatata tatttatgaa
     1681 ttgtaaatgg atatctaaga tagatgagag aaaaaaatgt catagagaag cagattcttc
     1741 tggatattgt atctttcata aagaaaataa gtctgatgaa gagatacagt tgatgatgga
     1801 tactattcat aaagaagaga taagtgaatt taatggattt gtttttgaaa atgaatttaa
     1861 tgcggaagaa atattaactt acaattataa aatactggat ttttctgaat ccatatttaa
     1921 acaaaaagca aattttaaaa aatatatttt taaaaagaat attattttta actatacaga
     1981 atttagagat aaagttttat ttaatggttg tatattctta gaaaactgtg attttaatag
     2041 aaccatattt agtaaaaact atataaatga ccgtatattt gaaaaagtga aatttaaagg
     2101 acctgatttg gtagttaata aagtagaaaa ttttccacga atggatggaa taatttttag
     2161 catgtgtaca aaatttgtgt taaagaatgt tgaatatgga aaaagtgagt atgagcatgg
     2221 caagataaat tatagaatag ctagaaatca agcaacaaaa attggagaat atgagatgat
     2281 aggattttac tattataagg aaagaattta tagtagtaaa ataatgaaac gttctaatta
     2341 tcctactttc agtgattatt tagttgaaaa attttttgac caaatagcac gttacacaac
     2401 aggttatgga gaaaagccat ggaatatatt attagtgatt atagcaataa taagtgtttt
     2461 tgctttatta tacttatttg taggcataga aagttcaaat agcacacttg tagctttaga
     2521 tataaacaat ataggtgatt actcactaag tgagattttc aaaatgtata tggatttatg
     2581 gtattttagc atggctacat ttagtacagt aggatatgga gatatggttg ctacaagttt
     2641 gattgggaaa gcattggcag gtatagaagt attttttgga gttacaatag gagcaatttg
     2701 ggcatctgtg attattaaaa gaatgataag gtaatatttg tgtaaatatg gataggtatt
     2761 aattactaga tataaattgg accaaatagg gaaaatttaa atataaatat acttaataat
     2821 ccaagaaggt gaaaaatttg aaaaatgcac taaaaaaaat tatatcatta gccatgattc
     2881 tagtttttat aataccaaat tattcatatg cagatggtaa atctgtagac aagtattcaa
     2941 aaacatctat acttatagac caagaaacag gtagagtttt atatagtaaa gaacctgata
     3001 tgaaggtacc attagctagt ttaagtaaga tgatgacttt tttacttgca atagaagcta
     3061 ttgaaaataa agaagtaaaa gaaactgata aggtaaaaat agacaaatct attgcatcag
     3121 taaaagggtc aagttataaa cttaaagatg gtgaagaagt gccacttata gagcttatga
     3181 gaggtcttat gattgtatca ggaaatgatg cggcaattgc tatagcaaaa catatttgta
     3241 aaacgaaaga agcatttgta gatagaatga ataaaaaagc aaaagaaata ggaatgagta
     3301 atacacattt tctaaatcca aatggtcttc caatatacga tttaagcaac cccaaaaaac
     3361 cagcaaaaga aaatatctct actgcaaaag atatagctat tcttggaaaa tacatgtttg
     3421 accattatga aaaacaagta acagctataa ctgatatgga gacatataca aattcagata
     3481 gaagttttga aaaaagcaat acaaatgcat tattaagaat tataccagaa gtagatggta
     3541 taaaaactgg atatactggc aatgctggat attgcttatc attttctatg gttgtaaata
     3601 aaaatgagaa aaatgagaaa aaacgtagag taataggggt tgtattaggt actaaccata
     3661 aaaataaaag aacctctgct tcattagctc tactgaaata tggaaaagaa aatttcaata
     3721 tgaaaaaagt aattgataaa gatagcttta ttggtaagaa atatataaaa ggaatgaaag
     3781 agttagaggt tacattaaaa gcaaaagatg aactttatac aatattaaaa gatgatgaaa
     3841 gtataaaatc agaagttaag attaaaaata tagagtaccc tgttaaaaaa ggtgatacta
     3901 tgggtgttat aaaatattat actagttcag gtgacttact tggaagtgta gatatagtaa
     3961 gtaataatag tatagataac gtatcattaa aaacaaaatt aaaaatgaaa tttgcaaatt
     4021 aaattcaaaa aatggtataa aatacacaaa aaaatataaa aatagaaaat atggaaaaaa
     4081 tattgttatt tcttcgcctc tgtgatatac tactagaggt gaaaaaataa aataaatcac
     4141 acacagcatt gggatcctta aaaggtgcct attacatggt tttttaggga gaagatcaag
     4201 ctggaggtat aaaaccagga ggtaagatat gtcagtaata tcaatgaaac aattattaga
     4261 agcaggtgtt cactttggtc accaaacaag aagatggaac cctaaaatgg ctaagtatat
     4321 attcacagag agaaatggaa tatatataat cgacttacaa aaaacagtta aaaaagttga
     4381 agaagcttac aagttcacta aagaagtagc tgaaacagga aagccaatct tattcgtagg
     4441 aactaaaaaa caagctcaag atgcgataaa agatgaagct gaaagatgtg gaatgtactt
     4501 tgttaatgaa agatggttag gtggaatgtt aacaaaccac aaaactataa aaacaagaat
     4561 aaataaatta agagaattag aaaaaatgga agaagaaggc gtatttaatg ttcttcctaa
     4621 aaaagaagtt ataaaattaa gagctgaaaa agaaaaacta gaaaaatact taggtggaat
     4681 aaaagacatg cctgaattac caggtgcaat gttcgtagtt gacccaagaa aagaaaacat
     4741 agctattcaa gaagctcata gattaggtat accagtagtt ggtatagttg atactaactg
     4801 tgacccagaa caattagact ttgctatacc aggaaatgat gacgctataa gagccgtaaa
     4861 attaataact ggtgctatgg caacagctgt aattgaaggt agacaaggtg ctgaggaaga
     4921 agtagctgaa gaccaagaat aataatttga caattaaatg gtaagggtcg cccttaccat
     4981 ttataatatg gaatagaata agtaggagga aacttaaaaa atggctaata taactgctca
     5041 aatggtaaaa gagttaagag aaagtacagg cgcaggaatg atggactgca agaaagcttt
     5101 acaagaagct gaaggaaaca tggaaaaagc agtagattta ttaagagaaa aaggattatc
     5161 aaaagctgct aaaaaagctg gtagagttgc tgctgaaggt ctagttgcaa tagaaatgaa
     5221 tgatgataat acagttgctt ctatggtaga agttaactca gagacagatt tcgttgctaa
     5281 aaacgaagac ttcaaagtgt tcgttaaaga tgctgcttgt atggcattag ctactgataa
     5341 agaagatata gcttctttat taggtgaaac tcataaagag ggaataactt tacaagaagt
     5401 tttaaataat agagttgcaa aaataggtga gaaattagac tttagaagat ttgctaaagt
     5461 tgtaactaat ggacaagttg ctggatacat tcacggtggt ggaaaaatag gtgttcttgt
     5521 tgaaatggaa acagaagcta gagatgctaa agttttagaa ttaggtaaag atgtagctat
     5581 gcaagttgct gctatgaatc ctaagtatgt ttcaagagat gaagtagatg ctgaatacat
     5641 agcacatgaa acagaagtat taactcaaca agctttaaat gaaggaaaac cagctaatat
     5701 agttgaaaaa atggttaaag gaagattaga aaaagaatta aaagaggttt gtttattaga
     5761 gcaaactttc gttaaaaatc cagatataac tgttaaacaa ttagttgctg atgttgctaa
     5821 ggcagtaggt tctgatataa aagttgttaa agttgtgaga ttcgaagttg gtgaaggtat
     5881 acaaaagaga gaagaaaact ttgcagaaga agttgctaag caacttaagt aataaccaaa
     5941 gagagaacac gcaggtgttc tcttttttaa aaaatagaaa aaattggata tgaaggattg
     6001 aaataggagg tttctgatgg ataaaccaat gtacaaaagg gtattgttaa aattaagtgg
     6061 agaagcatta gcaggagaaa gaggttttgg tataaataat gatgtagtta atgatattgc
     6121 tattgctata aaaaaaatac aagaaatagg tgttgaagta gctgtagtag taggtggagg
     6181 aaacttctgg agaggcagaa ccagtgaagg aatggataga actactgctg actatatagg
     6241 aatgttagct acagttatga atgcaatggc gctacaggat gcactagaaa atatagatgt
     6301 tgctacaaga gttcaaactg ctatagacat gagacaaatt gcagaaccat atataagaag
     6361 aagagcagtt agacacttag aaaaggaaag agttgttata tttggtgcag gaacaggaaa
     6421 cccttacttt acaacagata caactgctgc tttacgtgct gctgagatgg aagcagaagt
     6481 tatattgcta gctaaaaatg ttgatgcagt atatgacaag gatccaaaag tacatgcaga
     6541 tgctaagaaa tttacagaat taagttacat ggaagttata cagaaagaat taaaagtaat
     6601 ggattctaca gctacatctt tatgtatgga taataaaatt ccaataaagg tatttgagct
     6661 tacaacagag aatataataa gagcagtaaa aggcgaaaat ataggaacta ctgtaaaata
     6721 atgaagaatg aaggaggagt aaaattatga aactggaaat acataagcaa ttagaagaga
     6781 aaatgaatgg gacaattgat gctttaaagt ttgaatttgg aactataaga gcaggaagag
     6841 ctaatgcaca aatgctagac aaaataagag ttgattatta tggaactcca actccaataa
     6901 atcaaatagg tgctatatct gtaccagaac caagaatatt aatgatatct ccttgggata
     6961 aatctgctat gcatgaaata gaaaaagcga tagctaactc agacttagga ttaaatccat
     7021 caaacgatgg tgaggttata agactttctg tacctgcttt aacagaagaa agaagaaaag
     7081 aattagctaa aaaagctagt aaagctgcag aagaatttaa agttagaata agaaatgaaa
     7141 gaagagacgc caatgagaaa attaaaaaga tggaaaaagg cggagaatta actgaagatg
     7201 aattaaaaaa agcacaagat gaagttcaaa aaatgactga taaatttata aaagaaatag
     7261 atacattatt atctaaaaaa gaaaaggaca taatggaggt ataatattga aaaaatatta
     7321 taacctttta ggtcttcatg tagatgaggt aaaacaatat tttgatgagc aaaatataaa
     7381 ttataccgta aaatctattg aaggtaaaaa ggataaagaa aaacttgtag ttcctagagt
     7441 tataaaaata tctgagatag gtgatagtgt agaactgata atcacatact tttctgactc
     7501 cttagtttaa ggagtcagaa caatattgga ggcaaaatat gaataacaac attatatatg
     7561 atatagactt aaataacata cctactcata ttgctattat aatggatgga aatggaagat
     7621 gggcaaaagc tagatttctt ccaagaactg caggacataa ggctggagta gaaactataa
     7681 gagatatagt aaaagaatgc tcaaaattag gtgttaagca cttaacatta tatgcatttt
     7741 caacagaaaa ctggaaaaga ccgaaacttg aagttgacac acttatgaac ttattatcta
     7801 catacttaag aaatgaaata gctgaacttc accaaaacaa tgtaaaagta actgctatag
     7861 gagatataag tgctttacca aaaacttgta taagagaatt aaactcagct aaggaactga
     7921 caaaaaacaa tacaggagtt aacttaaatt tagcattaaa ctacggaagt agagcagata
     7981 taaaaaatgc tcttatagat atagtaaaaa attgtgaaag tggtaaaata gatataaaca
     8041 atattgatga agatattata aaaaactatt taagcacaaa gtctatacca gatccagact
     8101 tggtaataag aacaagtggt gaacaaagat tgagtaactt tttactttgg gaagttgctt
     8161 attctgagtt ttactttact gatatacatt ggcctgattt taaaaaagaa gaactacaga
     8221 aggctatata cgtatatcaa agaagggata gaagatttgg aggacttaag taaggagatt
     8281 attttatgct tacaagaatt attgcttcac tagctctggt tccattattt ctatttgttg
     8341 tatatggagg tattcctcta tatatagctg agacagcaat agtttatatt gcattgcatg
     8401 agttttataa ggcatttaaa ataaaagatg tacatccaat attcattata ggatatttat
     8461 tttctattta tttagctgta aaaaatattt ttaatttacc attagaatat acttatgcag
     8521 taatttttat tttattttta gcaagtataa tttatatgtt gatgggcaaa aacaatgtta
     8581 tagatgtttc gattacgttt ttaggagttt tttatatagg agtattttta gattttataa
     8641 ttataactat aaatggattt gaaaaaggta gtatatatgt atggcttata tttgtgatat
     8701 cttttatgac agatatattt gcatatttta gtggttattt acttggaaaa cataagctaa
     8761 ttcctaaagt tagtcctaaa aagactatag aaggagctat tggaggaata ataggaagta
     8821 cactttgttg tattttattt ggatatttat ttggcattga tttgcttcaa ttagcgataa
     8881 taggaagtat aggaagtgta atagctcaat taggagattt atttgcatct tctataaaga
     8941 gatatgtagg gataaaggat tatggaaaga taatacctgg tcatgggggt atattagata
     9001 gatttgatag tgttatatta gttgcacctt ttgtatatag tgcgattaaa ttttttatta
     9061 gataaagatt ggttggattt aaaagagcaa tctataattg ctagtaatga aaatagctat
     9121 gtaactttta aatgcagaga tattctgcag ttattagtta catggctatt ttcattgttc
     9181 aaaaatttag aagaccttgg ggaattttat aagaataagt attactttat aatagtatac
     9241 tatatttgta gtgagtatat tttttatact ataactatca ttaaaaaata tttttttgtt
     9301 taataatttc gatattttta gctttattat atatttataa tatatatgta taaatgtaac
     9361 taatttatta tccatttata aattttattt ttaaatataa ttaataagaa ataagtaaat
     9421 tattggtctt attgcacaaa aatagatata atttaagtaa tataccaaaa aaaaggaata
     9481 tatttacata atatgataaa ataaattcaa agagttaaat tgtaggtaaa tagtatttta
     9541 attatttatt aaaatataga ttatgtatat gccaaattta aaaagagaca taaagacatt
     9601 atacaaatat attattgggg tgatatttta taagaaaaga agtgatgcag taaattaatt
     9661 gagcaattta aaaatgattt aatgatagtg ttgatgaatg tttaaaaaga aattttagaa
     9721 aaacgtatat tgctgtttgt gttggaggtg atgaatttgt ataatttata gggtataaat
     9781 gattaaacta cttaagataa atacatatca ctgtaaaata ttgataatca ttttttataa
     9841 gagattgatt atttgcaaat tatatatatt acaaatgagg taacaataat gaagaaaaaa
     9901 tatgattttt taatttattc tgctatttgg ctagttatta ttattttttt tatattttct
     9961 ctatatcata gtatagagca tattaaaatt ataagtcaca ctggtactat tcgcagtgaa
    10021 actcaaaagg ttgttaagca agaattgaat aatgaaagaa atgatgactt gataaaacga
    10081 ctggataata ttctgataaa gctaagaaca ggtaatggag aaaatggatt tcaaagatgt
    10141 gataataaag aatttcaaca aaaactaaat caaatggatt ctatgtggga atctatgaag
    10201 aaagaaatta taaaagttag aaatggtgct tcaggtgata aattatataa attgagtgaa
    10261 gagtattctg tgttgtctaa tcaaattgtt tttatatctg aaaaacactc taatgcaaaa
    10321 ttgtattctt ttgctactgc actatttatt tatcttatat tctcaacaat aagtctttta
    10381 atttgggaat attataataa aaaaaggttt aaaagaatat tttatactga taatttaaca
    10441 aaaataaaaa atcaggttgc atttgaaaac agagcgattg aaattttgta caatgcatct
    10501 aacaaagagt atgtactgct aaatattgat attgacaatt ttaaatatat taatgataca
    10561 catggatatg aatatggaga taaagttcta ataatagttg cagctgcact ttctaaaact
    10621 tttaatatca aagaaacttg tgcaagaatt ggttcagata attttgtaat acttgcaaaa
    10681 tatagagatt ctcttttaga ggatattcgg gaaatgctaa ctaatgctat aatctctgaa
    10741 ttagatatga atgtaactca aactatatct tattgtatag gagcgtactt agttgaaatt
    10801 gataatttag gttataaatc catcaattct ataatggata aggcaaatat tgcacacaag
    10861 gtaagtaaaa caagaggaat ttcctccaca gtatggtata atgaaaatct gttaaaacag
    10921 ttacaaatgg aaaatagtat ttataattat atgtataaag cgatagaaaa tgaagaattt
    10981 catatgtatt tgcaacctaa atttcaaata tcttctttaa atgttgtaag cgcagaagct
    11041 cttgtaagat ggttttctcc agaacttggt tttttatcac cagatgaatt tattccattg
    11101 tttgaaaaaa gtggatttat tattgaattg gacttttata tgttaaaaaa agcgtgttca
    11161 tttgtaaaaa aaacgtttat gaaaaaaaat caatatacat atccgattgc agttaatttt
    11221 tcaagggtta ctatttacca aaatagcttt tatcaaaggt ttttagatac tgttaaagaa
    11281 tatgaaattc cttttaagta tatagagata gaggtaactg aaagtgcttt taatgaaata
    11341 tctcaaccag ttatttcgat tttagaggag cttaaaaagt taggattttt aatttcgatg
    11401 gatgattttg gatcaggata ctcttcatta agtcttttat gttctctatc tattaatgga
    11461 ttgaagttag ataaaagctt acttaaagaa acatttaacc gggagaaagt atatagtatt
    11521 attcagtgta ttattgaaat gtcacatcgt ataggcatgt ctgttgtatg tgaaggtatt
    11581 gaaactaaaa aagacttgga atttttaaat acagttaaat gtgatgtagg acaagggttt
    11641 tatttctcta aaccgataga agaaaaagaa ttttttaaca aatatgtaac taaaaaataa
    11701 tataattatt tttatcagtc tatacattga
//
LOCUS       DAEPLF010000103        11699 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEPLF010000103 DAEPLF010000000
VERSION     DAEPLF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11699
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.103"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            563..715
                     /locus_tag="KR314_002294"
     CDS             563..715
                     /locus_tag="KR314_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328675.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHYPAFAGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(1007..1711)
                     /locus_tag="KR314_002295"
     CDS             complement(1007..1711)
                     /locus_tag="KR314_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0328676.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1702..3309)
                     /locus_tag="KR314_002296"
     CDS             complement(1702..3309)
                     /locus_tag="KR314_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBH0328677.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(3563..4741)
                     /locus_tag="KR314_002297"
     CDS             complement(3563..4741)
                     /locus_tag="KR314_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328678.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(4967..5461)
                     /locus_tag="KR314_002298"
     CDS             complement(4967..5461)
                     /locus_tag="KR314_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH0328679.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5462..6223)
                     /locus_tag="KR314_002299"
     CDS             complement(5462..6223)
                     /locus_tag="KR314_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH0328680.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            6946..9780
                     /locus_tag="KR314_002300"
     CDS             6946..9780
                     /locus_tag="KR314_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0328681.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHVSIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNISKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNIRKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            9823..11067
                     /locus_tag="KR314_002301"
     CDS             9823..11067
                     /locus_tag="KR314_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH0328682.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKV"
     gene            complement(11508..>11699)
                     /locus_tag="KR314_002302"
     CDS             complement(11508..>11699)
                     /locus_tag="KR314_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBH0328683.1"
                     /translation="VGSKATYKTAIDEAQAILDKADATQKEIDDAVTALNTATATFEA
                     GKVPTTIALMLSRILGFMK"
BASE COUNT         4190 a         1873 c         1441 g         4195 t
ORIGIN      
        1 caatagagtt ttgtaatacc taatttaata tatttataat taactatctt attatgtgtt
       61 tacaattaat tctcatataa atgacaaatc tccacctcaa ttaagtattc caatcatata
      121 tgaagataat acttcattca tttaaaaata taaaactatt atatattaaa atttcttcaa
      181 taataaatta tctagattgt tactttgata tattatataa tatcacaaag gctgatataa
      241 aaagacaaaa agggtaattt ttttatatca aaaaaaatgt taaattgata ttatccatat
      301 ataaattgat attatccata tagatgctat atttaacaat tctacttact tcccttacac
      361 ttattagcat atagttatta agagtttgtt gcaaaacaac acttagcaca ctttaaaaag
      421 tgtggtctaa taagtaatca acgaagaaga cataataaag cataaaatat gatatacttt
      481 tcctgtcatc aaaactgatg attttactat taatatctgc taaaatatgc ttaataaatt
      541 aaattttagt gggagaatag ttatgaaaat atttagtttc ttgttgtttg tagtgggaat
      601 atgtttttgt attgctggat ttatgggaca ttacccagca tttgctggtg caacattgag
      661 tttttttgca actacaatga tagctgtaag acaagtcaaa actaagaaaa aatgaaatag
      721 gcaaatccta actttacaag ttaatattac ataagtaatt agtctaaaat tggatacttt
      781 ttctattaca gaaatacatc atccactttg tattctctta aaaaaataaa atagctatgt
      841 aaattcaaga ttagatagat taactctcta tccatttcat tgtaaaatat acatagctat
      901 ttgtattcga tttaaaatac ttagattttc ttacataaca aataatttct atctgattat
      961 tactcatatt catttagtaa tcactcagca actaatcatt tagtatctat tcatccgcca
     1021 tacggtaacc taccccaacc tcagtcaaaa tatattctgg ctgagcagga ttcttttcaa
     1081 ttttccttct tatacttgcc ataaaaactc taagcgactg attttcactt tcatagtaat
     1141 ttccccaaac tttatctatt ataaacttat gagttaagac tcttcctgaa tacttagaca
     1201 tcaactcaat aatcttatat tcgattggag ttaaatgtat ttcttcacca ttaattgaca
     1261 caatatgatt ttcataatct atctttaggt ttttaacttc aaaagacttt ggagcatcat
     1321 tatttatatt atcctgttga gctttatttc taagagccac acgtactcta gccaaaagct
     1381 ctgttacact aaaaggttta gttaaataat cgtctgctcc tccatctaaa gcttccacct
     1441 tatccctctc atgctctctc gctgaaacaa caattatttt attagtcttt gcacatgccc
     1501 taactcgtcc tattacctca atcccatcaa tatctggtag tcctaaatcc aaaattatca
     1561 aatctgggtc aagagaatgt ataagtgata tagctgttcc tccattatct gtctctacac
     1621 acctataatt ttgagtttca agagatactt ttataaattt acaaattggc ttatcatctt
     1681 ctacaattag aactaatggc tttatttcca cttgtctttc ccctcctcat catacatagg
     1741 tatgctaaat ctaaatgtag ctcctccaga aggattatta aatgcgacaa tttctccacc
     1801 atgagcttct actatagatt tacatatctc caatccaaga ccaatacctc ttctttcaag
     1861 atttctagac ttattcttag tataaaacct attaaaaata ttaccgatat cttcatttgg
     1921 tattcctttt ccattatctg caacttcaaa tagcacttga tttgactctt tttttacagt
     1981 tagttctatc ttagaatttt taggtgtatg ccttatagca ttatcaatta aatttactaa
     2041 cacttgttct attaataata catccacatg aacgattatt atctcatctg gaatattggt
     2101 ttttatgtct ctactattgg caaacttttt gactcttaat atagattcag ctattatttc
     2161 atcaacaact tctggtcgtt tttcaatatc caatttccct tcatctattc tagtcatact
     2221 aagtatattc tcaacagaat gagttagcca actcgtatct tcatatatat ttttcaataa
     2281 ttctactcta gtttctttat ctatcacatc atcattttca agtattgttg aagacgaacc
     2341 caatatactt gtaagtggag ttcttaaatc atgagaaatt gacctaagca aatttcctct
     2401 taatctctct ctctctgcct caagatttgc ttttttattt ttctcaaata aattttctct
     2461 atcaattgcc aacgcaacct gtgttgaaac tgcttctaat agtatctttt cattttctgt
     2521 aataatatct atcttaaaac acgcaacacc tataacacca agaacatttg attcttgacc
     2581 ttttataggg aaataatatg cactgctgtt tttgcaatat tttgtcccaa ttccaactgg
     2641 gttacctgtc ctaaaagctt cttttatagc ttctttttct aatactgaat ggaatatatt
     2701 ctcacttcca tcattgttac aaatataaat acttggctta cctagattat tattctcatc
     2761 tgcaatagtc actattacag ttctatttaa tatatttact aaattagacc cacaaaattc
     2821 aataatttga tttttatttt ttgctttaag caacgttttg ttattctcat agagcaatct
     2881 tatcattttt tctctcttat gagaaagtct cacttctctt tttatcctag aagttaaagt
     2941 acttgttata attgctgcca ttagcataat acaaaaagtc ataggataat cttttctata
     3001 agctaaaaaa gtatataatg gatgcgtaaa gaaaaaatta aatgatagca ctcctattac
     3061 tgaagaaagt attccaaaaa aatatccata ggttatactt gtagcaaata gtacacctaa
     3121 tatgtatact agtataatat tagattcatt aaaacctacc ttttcaaaaa ctactgatat
     3181 tccagtagca attgtcataa ccaatatagt ttttatgaag tccttaataa ttatcttttt
     3241 actgagatag ccatcttcat cataatcata ctcttttaag tcttcatgtt gtagtcgttc
     3301 ctttttcatt tactacaatt cctccttatt gttactgttt aaattggctc tattttatat
     3361 aatacaattt tatcataata aattcaatca atacatttaa ttaatgtact taaattacta
     3421 aaaggtgctg tcttaaaatg aaaataaatc tctatttcaa aacagcacct atatatttaa
     3481 attactaatt tagtttttac atacttaaat caatagtttt attcaatttt ataaattata
     3541 atacttcaat tatggaacat ttctatccaa caagcataaa tataaagtgg gcacatatac
     3601 ctgcacacaa accacctatg gtatgcgaaa gtatcgcttt accagccaat ggtcttgcat
     3661 ctaaagcatc catcatacct atgtgtgtac ttaaatatcc actccaacac atacccattg
     3721 ctgtgaatac tgctatatca ttaggagtta tagcgcctga ttttataaac tctggtacta
     3781 aagatattgc tgctccaaca gctcctaatg cagttacagg gaatgctatt gcctctggtg
     3841 atgtgaatcc aaataatggt tctattatga agctaatctt atcaccaata gcaggtaata
     3901 attttatacc ttcatatgca gctcctgtgt aaacagcttg acctgtagca gggtctgtag
     3961 atggtccaaa agttaataac ataactaatg tacaaactac taaaactcct ggtattatag
     4021 ccatacccat ttcaacacct agcttaccac cttcaagtat agcatccaat gttctttgga
     4081 atacattacc atctcttatt tctctaaact cttcaagttt tgcaccttta tcatttattt
     4141 gcttttcagt atcattttta gggtcataat tataatactt tttagtaaaa gtcaacataa
     4201 ttctaacact aataatacta cctattattg ccccaacatt acctattata gctggtagta
     4261 catattcttt accttgagct atcataaaag ttgttacaat aagtcccata ccaaaggctg
     4321 ttcctaaatt acataaagca ggtacttgat acttcttaaa atattgtgta aatgttttgt
     4381 ctttagcaaa tggtataata gctggattat cagataaata tgttgcaact actcctgcta
     4441 tactagcacc aggtaagccc catataggtc tcataagacc cttaaatatc ttattaacta
     4501 aagatattac tccaaactct gaaagcaacg cactaagtgc acctgcaaga actgccatag
     4561 ccattattaa aaatactgtg tctaataaaa gcgcatgtgc tgtactcatt attactttaa
     4621 acatcattcc tgcacccatg atgcttccta catatccaaa tcctaccgcc aatagtacaa
     4681 taaatacgaa ggtttccata cttactgcct tgactcttct cttatccgac agcttttcca
     4741 tcttgaaatc ttcccccttt taattttaaa ttaaggttcc taacattggt acaaattttt
     4801 tggtacaaac tgagttctaa aacttacact cgtaatgcaa gttgtcagtt ttttgaaata
     4861 aaaaaaaatt acgtttatag aataagaata tcctctttaa tttggatact cttattctat
     4921 tttatgttat tatattatca tccttttgtt taaaagtcaa ttttttctat ttttgttgtt
     4981 taattttatt tatactcttt ttatattttt cattttcttt tatctttgta agcactccat
     5041 ataagttgac ttttctaaat acagtagtta taaataccaa tcctaatgca attgaacttg
     5101 cacatgataa tgtactcacc ccatattcta cagcttttat ataattattg gtcaaaacag
     5161 aatacatagt attatatatt ccataaccag gtactaatat tattaaacaa caaacactta
     5221 atgtagttgc tggagtcata taaattcttg cacataactc acaatatatc gcaaaacaaa
     5281 ctgtagctat aaaaaaacat aagttttctg ttactcccat atttaaaaga aatttatatg
     5341 aaaaccaagc tatacttcca cctattcctg ctagtattaa gttttttcct ttcatattaa
     5401 atagcactcc aaagcttaag gcagtaaaaa aagctgctat tacttctata aacaaatgca
     5461 cttatatccc ccctaacttg aagtataatc caagtaccaa gaacattcct actgctatag
     5521 atacacctac aaaaaatact tctgctaatt ttgcaagacc agaaatcaac tgtccatcta
     5581 acaaatctct taatgcatta gtcaaagcaa gcccgggaac taagagcatt attgctcctg
     5641 ctattaaatg gtctaacgtc tttataaatc cgagctttaa aattaaaatt gaacaagaag
     5701 ctataataac tgctccaatt gcatttacaa aaaagtcatt aagtgaagat ttacttaaat
     5761 acataataac aaacttagtc agaatcccaa cagtaaatgc agctataaat tcttccacat
     5821 ttcctccaaa taatatcgaa aaagttgctg ctgcaatacc tgcaaaaaat attgttatta
     5881 aatccgagta atagttatct tcacttattt catctaaaat tttctcacat aattcaatat
     5941 ctaaattatt tatacttatt gctcttgata atgagttaac ttcatgaacc aagtttaggt
     6001 ctactcttct tgagcttatt cttttaattg ctgaatgtaa tttcccatct acaaatacag
     6061 atgccattac agctgttgga gttgcaaata catctacgtg gtctattcca aaagattgac
     6121 atattctagc tatcgtttct tcaactctat aggtttctcc tccactttgc aacatagctt
     6181 tacctgcatt tgaggaaaag tttaaaattc tatctatatc cacaaaacat atctcccatt
     6241 cttttatagt attattcata ttttaataca agatattatt tatttaaact attttaaata
     6301 attcatatcc aatatttaca ttttatgaaa aaacttgttt tttatgcttg tcaaaatcaa
     6361 attttaagct tgtttacacc ataaaatggc tgaattaata tttgacaaac actttcaacc
     6421 atttatataa ccttctaaat tgtataactt tttactacaa ataaaattat atctctttta
     6481 attctttctc ttgagtattt aagcttatag caacttcata tttatatatg tacaatataa
     6541 aacattattt ttatgataca aaatataatt tgtaaacgta tatatgtatt aactgtgtat
     6601 atataaatta ttcttaacct tatcagtttt ttcatatatc ttcatctata tcgaccatat
     6661 attaattaca ttgaccatat actaataaat gatattacat agactgaagt atattaaaaa
     6721 acttaaattt agttatttaa caacataata ctctaattta atctataaaa atccatacct
     6781 aatataatgc aactcttatt ttaacaccat ctattacaca aaacaacata ttaaaaagta
     6841 aaatacaaaa tatccactat aaaccttatt ttaatactga atatatatta taatatattt
     6901 aatgtagctt aaatatagtt taaacataaa gaaaggaaat ggtatatgct aagaaaagat
     6961 aaagttctat tatctctaca taaattatgt gaaaatatga ctttaaaaga ccttcaatca
     7021 gataaaacag gattcactac aatagaaatt gctaaccatt catctcttga tagaagtaat
     7081 accagtaaag aattaaataa cctatataat gaaaaaaaag ttctgaaaat atctggtaag
     7141 ccaattttat ttctacatgt tagtattata gaaaatttaa ttggccgtac tttgaacagt
     7201 gatgaatttt ctataaataa ttgtaatgag ctacttaaat caagtaaaaa tgactcagaa
     7261 gatatttttt caacattact tggacatgat tctagtctta aactacctat agaaaaagct
     7321 aaatctgcta tactctatcc tccaaaaggt cttaatacgt tactcatagg acctactggt
     7381 gttggaaaaa gtacatttgc cgaaacaatg tacaaatatg ctgtagaaag taaaatcttt
     7441 tcttctgaaa gcaaattcgt agtatttaac tgtgctgaat atgcagaaaa tgctaactta
     7501 ttattaagta atttatttgg ttatgtcaaa ggttctttta ctggtgctaa taaggataaa
     7561 ttcggcatag ttgcacaggc tgatggaggc atattattct tagatgaaat acacagatta
     7621 ccaccagaag gtcaagagat gctttttctt ttgatggaca aaggcatata cagacgatta
     7681 ggagaatctg acattgtgca caaagctaat gtctttattg tgtgtgccac aacagaagat
     7741 atacattctt ctctgcttgg gactttttta agaagaatcc ctgttacaat caacctacct
     7801 gctttacagg ataggtcttt aaaagaaaga ttatctctta ttaaatactt ctttattata
     7861 gaatcaaaat acaccaatgc aactataaaa gttcacaaag atgtaattaa atcattactc
     7921 ctttatgatt gttttggtaa tataggtcag cttaaatctg acatacaact tatgagtgca
     7981 agagcctttt taaattacaa aactggactt aaggataata tagaaataga tttatccctt
     8041 gttcctgatt acatctatat tggtctttta aaattgaatg aaaatcgtag taatctaaat
     8101 aaattatcag aacttgatta tatgcttttt tatgaattta atggtaataa cttagaagtt
     8161 gaatatacaa aagaactatc taaatacaat atagatacat caaacaatgg atttaaatac
     8221 tctacagaga ttccttatct ggaaaatgat ataaggagta tgtttataaa ttacatggaa
     8281 aagtactcta caaaattact ttcgatatct tcagtaactc attctgatta tacaagtaat
     8341 gaaattttta aagtaatcaa tccaaagatt tactatgctg ttgaacattc tttaaaagag
     8401 gccgaaaaaa tatcgagaaa caaatacaca aaacaaactt atattgctct ttcaatgcat
     8461 ataagtgcac ttatagaaaa tataaatcaa gacacctata atcctaagta caatttatat
     8521 aatgataaaa ttttcttaga taaagattta aatatatcta aaatagtttt aagtataatt
     8581 gaaaatgact tacaaacatc tttacctaaa gatgaagtaa aatttatatc catgtttcta
     8641 aatagcatgt atcttgaacc aaacaatcaa tctgaaagta taggagtcat aatacttgct
     8701 catggaaatt caacagcaag cagtatgtgt gatgtagcaa attcattgct tggtacaaac
     8761 cattgtaatg caattgacat gcctcttgat gtaaaagtaa gtaccatact tgataaaact
     8821 atagagttgg taaaagaatg tgacgaaggt aagggtgtac ttcttttaac agatatgggc
     8881 tctcttatag cttttggaga acttatttca gaaaaaacaa aaatcaaaac tcgctcaatt
     8941 gaaatggtat ctactccaat agttttagaa gcagtaagaa aaagtacatt accagaaatg
     9001 actttggatg aattagctaa ttccttaagc tctttaaatc catatattgg acgagcagca
     9061 atcgattcaa cagaaataaa ctatcctgaa aagtcaagag agtatgtaat aataactact
     9121 tgtataacag gatatggttc agctatgaaa gtagcagact ttataaattc ttcacttaaa
     9181 aatattcgta aatacaatat taatttagtt cctcataata tagaaagttt taaaatgtat
     9241 aagaataaat ttagtaatac taaaattctg gcagttgttg gaagtgtaga ttttaatgta
     9301 gccaacaccc cttttataca catagaggaa ttactaaatg gatatggcct agatatgcta
     9361 aacaaaataa tagaaggtac attcaatgcc aatagtatta caaatataaa agaaacaaat
     9421 ctatcataca acagtttaat aataaacttt atgacccaaa acttagaact tttggactct
     9481 gttaaattat tcaactatgt caattcttcc ttatttacaa taatagaatt aatagatgaa
     9541 aacttaaaga gcaaatttaa gctaggatat atactacatt gctcttttat gatagaaaga
     9601 tgttttaaaa atacaacttt tccatataat aacattactg aattaattag taaaaatcat
     9661 gaactatata gtttaatacg tgaagccttt aagccaacag aagcttattt taatattttg
     9721 ataccagacg aagaaattgc ttatataata gacatgatta atgagtatct aaataaataa
     9781 aatttatagc acttattttg tgcatattaa ttaatatatg aaatggagga cgagattttg
     9841 aaaggaaaaa taaaacagtt aacaatactt gcattaattt ttatatttat aactcctgta
     9901 tttgcatttg cagatactcc accagtacca aattcttcaa gagcagcact acttatagac
     9961 caggaaacta aaagaatatt atttgaaaaa aacattgatg aaaaaatgcc attagcgagt
    10021 ttaagtaaaa tgatgacctt tttattggca atagaagcag ttgataagaa tcaagtaaaa
    10081 gagactgata tggtgaaaat agacaagtct acagcttcag taggaggttc tacttgtaaa
    10141 cttaaagatg gtgatgaaat atctctagga gagcttatgc agggtctaat gctagtatct
    10201 ggaaatgatg ctgctatagc catagctaaa catattggta agactgaaaa gaattttgta
    10261 aatatgatga acaagaaagc agaagaaata ggaatgattg atacgtatta ttttaatcca
    10321 aatgggcttc ctatatatac tgacccagaa cataaagaac ctcctattga aaatatgtca
    10381 actgctcacg atatagttac acttggaaag tatatgtatg accattatga aaatcaagtt
    10441 acaagaataa ccactatgca agtatataat gatactaaaa aggattttac acattataac
    10501 acaaatcccc tacttgtttc agtacctggt gttgatggaa taaagacagg ctatacagat
    10561 aatgctggat attgccttgc attttctatg atggtaccta aagatgctaa aaacgaaaga
    10621 aatcatagat tgataggtgt tgtacttggt gatggtaaca agaaaaatag aatttcttct
    10681 tccgctactt tattgaaata tggtaaagat aatttccatt ctaaaaagat agcacataag
    10741 ggtgatatta tagagactcc ttgtgttgat ggtattgatg attttaagat aacagttaag
    10801 gttgataaag atttgtatgg tgttgtttca gataacgaaa atatcaatcc taaggtagtg
    10861 tttaaaaata tgaattatcc tatccataag ggagatattg ttggtgttgc aaagtattat
    10921 aatgactctg gtaaatttgt tggaagtgtt gatgtaaaaa gtgaaagtaa tataggatgt
    10981 attcctttga aggataagat taagattaag gttgctaaga taaataaaaa acttgagatt
    11041 aaaaattctg tttgttttaa agtatagtaa agcatactat taaaagagta tattaaattt
    11101 ataaatactc cactttgtgg agtttttttg ttttttacaa aaaattaaaa tagtggcaac
    11161 aattgaggat gtgagggaga gttgccacta ttttgtatag ggtattatat tatgttttta
    11221 agctagtttt actatcttct atgtttatat aataactctt gtgtgtgaat ttaattgata
    11281 attaatatga aaattatgtg aagttttgat attaggttat actttgaact ttatctaaca
    11341 ttttaaattt tatatctcta aattatctaa aattactata tcaattaact ttacatgata
    11401 aaaaggctgt gtataaattt atttttaaaa attttactca cagccttaaa ttaaattttc
    11461 tatttaaagt atatataaat tttattaaat aatttgcttt ttatttctta tttcataaat
    11521 cctagtattc ttgataacat taatgcaatt gtagtaggta ctttccctgc ttcaaatgta
    11581 gctgttgctg tgtttaatgc tgttactgca tcatctattt ctttttgtgt tgcatctgct
    11641 ttatctaata ttgcttgtgc ttcatctatt gctgttttat atgtcgcttt tgatcctac
//
LOCUS       DAEPLF010000104        11665 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEPLF010000104 DAEPLF010000000
VERSION     DAEPLF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11665
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.104"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(3..905)
                     /locus_tag="KR314_002303"
     CDS             complement(3..905)
                     /locus_tag="KR314_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0328684.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            1260..2438
                     /locus_tag="KR314_002304"
     CDS             1260..2438
                     /locus_tag="KR314_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBH0328685.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINSGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            2447..3103
                     /locus_tag="KR314_002305"
     CDS             2447..3103
                     /locus_tag="KR314_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBH0328686.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            3103..3768
                     /locus_tag="KR314_002306"
     CDS             3103..3768
                     /locus_tag="KR314_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBH0328687.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     ILGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            3873..4649
                     /locus_tag="KR314_002307"
     CDS             3873..4649
                     /locus_tag="KR314_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBH0328688.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            4711..6045
                     /locus_tag="KR314_002308"
     CDS             4711..6045
                     /locus_tag="KR314_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH0328689.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVLLNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKVGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSKLEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            6542..7468
                     /locus_tag="KR314_002309"
     CDS             6542..7468
                     /locus_tag="KR314_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBH0328690.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPNNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSENFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(7860..11399)
                     /gene="nifJ"
                     /locus_tag="KR314_002310"
     CDS             complement(7860..11399)
                     /gene="nifJ"
                     /locus_tag="KR314_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBH0328691.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         4035 a         1884 c         1772 g         3974 t
ORIGIN      
        1 ttttaagagt tctttttact aaagaaatct ataataaatt taaatacatg ttggtctaca
       61 tggctataac gttctttttt aggtctacat agtataactt tccatgtaat aggatgatta
      121 aaaggctttt ctataatttt gctattttca aaaaaatcat gaactggtga tggtaaaatt
      181 gttataaaat cagaatttgt ggttgatagc aacaaaaaat cccatgaacc agacattata
      241 tatcttttta acttaacatt ttcatcagta aatttttgca ttagcttatg atgtatcata
      301 aaagtaggat taaatattgc aagtaattgc tcattcaaat cgttccaatc tatcttatcc
      361 ttcttagcaa gtggatgatt tatatttaaa aatgctgtaa gttcatcttc ttgaagcaca
      421 tgttcagtta ctacactttt atcaatttta tgtggatgta acagtactgc ataatctagt
      481 tcctgcaaga taagcattct atttaagtca tatgcaccct tctctactat atcaaattct
      541 atatctggat tatttgcaac taactgtgca actacatctg aaaatgctat tccaagtata
      601 agtggaggta ttcctattct aacttttcct ttaaattgaa ctgaatcttc tcttaaatct
      661 tccatcatat ttttgtattc atttataata tgttctgcat ttacataaaa tctttctcct
      721 gctggtgtta acccatttaa cctaccttta tacctttcaa ataaatatac atcttcactt
      781 tcttcaaaat tttttataat ttgacttaat gctggttgag atatgtgaag tgtcttagat
      841 gctattgata aattaaaacc agatttcact atttcaacaa aatatttcaa ctgttttata
      901 tccattttaa acccccttcc ccaaaagtat aatttagatt atattttttc ttctaatgat
      961 attttatagg aaaatcaaca taatttcatt aatattttaa caaaatattt ttctaaatct
     1021 actataaatt tttatcctat aaatttttct tatacactat ataaaaaatt agacttagac
     1081 aaaatgcttt tttcgccgta atctataatt agaaacagga aaacctaaag cattaggaat
     1141 aaaagtaatt gaaatactta aaaacaagct tgtattaaaa aagtacttta ttatagtgtt
     1201 ccctaatttc tttattcaga aaatttttaa ttatcattca agaaaggtaa ctattagata
     1261 tgaaagatgt agtaatcgta tcagcagtca gaacacctat aggaagtttt ggaggtgtat
     1321 ttaaaaatac ttcagctgtt caacttggaa ctatcgctgt taaagaagcg atatcacgtg
     1381 taggacttaa tttatctgaa atagatgaag taataattgg aaatgttctt caaacagggc
     1441 ttggacagaa tgttgctagg caaattgcta taaattcagg tataccaaat tctgttccaa
     1501 gctacacagt aaataaatta tgtggttcag ggcttaaaag tgttcagcta gctgcccaat
     1561 caatcacatc tggtgaaaat gatgtggtaa ttgcaggtgg tactgaaaat atgagccaag
     1621 ctccttatat cgttccaact gctcgctttg gtagtaaaat gggtgacata accatggtag
     1681 atagtatgct aacagatggc cttattgatg ctttcaatca gtatcatatg ggaataactg
     1741 cagaaaatat tgcaactaaa tttgaattta ctagagaaat gcaagataaa ttggcattag
     1801 aaagtcaaaa taaggctgag aatgctataa aaaacaaccg ctttaaagaa gaaatcgttc
     1861 cagtagatgt tttaatacgt cgaggaaaaa tagaaactat tgataaagat gaatatccta
     1921 aattaggcat gacatttgaa ggtttagcta aacttaaacc tgcattcaaa aaagatggta
     1981 ctgtaacagc tggaaatgcc tcaggtatta atgatggtgc ggctatgtta atactaatga
     2041 gccaacaaaa agcagacgaa ctaggtatta gaccacttgc taaaataaaa tcctatgctt
     2101 cagctggtgt tgaacctgaa gtcatgggta ctggtccaat ccctgcaact agaaaagcac
     2161 tgaaaaaagc tggacttagc atcaatgata tagacttaat agaagcaaac gaagcgtttg
     2221 ctgcccaagc attagctgtt aaaaatgaac ttcaaataga ttcatcaaag ttaaatgtta
     2281 atggtggtgc aatagcatta ggacatccaa taggagcaag tggagctaga atacttgtta
     2341 ccttaattta tgaaatgcaa aaacgtaaag ttgaaactgg acttgcaaca ctttgtattg
     2401 gtggtggtca aggaatatca atggtggttt cacgatagga ggaaatatga ataaaatagt
     2461 tagcattgat gaagctctat ctcatgtaaa agatggagat acaataatgg ttggtggatt
     2521 catggccaat ggttcaccag aaaatttaat tgattattta tgtagtaaaa atataaaaga
     2581 tttgacacta atttgcaatg atactggctt tattgacaaa ggagttggca aaatggttgt
     2641 aaataagcaa tttaaaaaga taattgcttc tcatgtagga ttaaataaag aaactggaag
     2701 acaaatgaac aacaatgaaa cagatgttga gttagttcct cagggaactc ttgctgaaca
     2761 aatacgtgca gctggttatg gtctaggagg tatactcaca ccgactggta ttggtactct
     2821 tgttgaggag ggaaagcaaa aaattgttgt cgatagcaaa gaataccttc ttgaaaaacc
     2881 aatttatgca gatgttgctc ttctatttgc atcaaaggtc gataaagcag gtaatctagt
     2941 ttataaaggc tctatgaaca attttaataa tttaatggca tctgctgcca aaattacaat
     3001 agttgaagca gatgaaatcg ttgaaattgg tgacattgac ccaaatgaag ttaatacacc
     3061 aggtattttt gtgaattaca tcgttgaagg aggtaattta taatggataa attagaaatg
     3121 caagaatata ttgcaaatag agtatctaaa gaattaaaag acggagccgt tgtaaacctt
     3181 ggaataggtc taccaaccaa agtagcaaat tatattccgg ataatgtaaa cgttatctta
     3241 caatctgaaa atggcttttt aggtttaggt tcagccgaag atggaaattc aagtgatgaa
     3301 actattgtaa atgcaggtgg tcaaccagtt actatacttc ctggaggatg tttctttgat
     3361 agtgctactt cttttggaat aatcagagga ggtcatgttg atataactat tcttggcgca
     3421 cttcaggttg acaaatatgg caatatagca aactatatga ttccaggtaa gatggttcca
     3481 ggtatgggtg gagctatgga cctagtaact ggagctaaaa aagttattgt agcaatggaa
     3541 catacttcaa aaggttctgc aaaaatatta aataattgta ctcttccact tactgcaaca
     3601 aatgctgtag atttgatagt tactgaaatg ggagttatgg aagtaacatc agatggaatt
     3661 ttacttaaag aaataaatcc agctttcaca ttagatgatg ttatttctgc tactgaagct
     3721 cctttaatat tgtcagattc attaaatgga aaaatatctg ttgtttaaaa taattaaaac
     3781 tagaaaataa aaatctctta gacgtattac atataaattt gtattgctta aaatattaat
     3841 ataaaaataa aataaaattg gaggaaatga aaatggttaa agataaagtt gtattcgtaa
     3901 caggagctgc aagtggaata ggtaaacaaa tagctgaatc gttcttaaaa aatggtgcta
     3961 aagtaatgtt ctctgatata aatcaagaag ctttaaataa taccataaca gagttacaaa
     4021 aaaaaggtta tgattgcatg agtgtaaagt gtgatgtaac taaggaagaa gaaattaacc
     4081 acgctataga taaaactgtt gaaaagtatg gcagattaga tatattaata aacaatgctg
     4141 gacttcaaca tgtttctatg atagaagatt tcccaactga taaatttgaa tttatgataa
     4201 aaataatgct gacagcacct tttatagcta ctaaaaaagc atttcctata atgaaaaaac
     4261 aaggctttgg tcgtataata aatatggcct caatcaatgg tgtaatagga tttgctggaa
     4321 aggctgctta taactcagca aaacatggac ttattggact aacaaaagtt gctgctcttg
     4381 aagctgccga ttctggtata acagttaatg ctttatgccc tggatatgta gatactccac
     4441 ttgtcagagg tcagtttaat gacctttcaa aaactcgtaa tataccatta gaaaatgtct
     4501 tagaggaagt tttgtaccca cttgtaccac aaaaacgcct attagatgtt caagaaattg
     4561 ccgactatgc tatgttccta gcaagtgata atgctaaggg tattacagga caagcttgct
     4621 tactagatgg aggatatact gcacaatagt tattatattt agggcttgaa gaaagtattc
     4681 ttcaggtcct tctactaagg agggatattt atgagtatta tcgcaccaat tggtataatt
     4741 attggtatca tagctattat atatttttca gttaaagaaa ttcatataac tatagctgct
     4801 ccaatagcaa ctttaatagt tgttctctta aataaaatgg atgttgtaac atcaatgcta
     4861 ggtgctgaca aaaacaatta tatgggtgca cttggaaact atataatgag ttattttgca
     4921 atattcttat taggctcaat acttgctaaa tttatggaag aaagtggagc cactgtatca
     4981 atagctgatt ttattttaaa taaagttggc tctaatcatc cttatagagt tttagttgct
     5041 atatttatag taagttttat attaacgtat ggtggtataa gcttatttgt agttatgttt
     5101 gcagtcattc cacttgcaag aactctattt aaaaaacttg atatttcttg gaaccttatt
     5161 cagataccac tttggcttgg tattgcaaca attactatga ctataattcc tggaacacca
     5221 gccattcaaa atgttatacc tattcaatat ctaaatacgt ctcttactgc agctactata
     5281 ccaagtattg taggtagtat aggttgtgct acgtttggac ttgtctatat gaaatatgct
     5341 ctaaacaaaa gtattagaaa aggtgaaact tacgcaactt acacttcaaa attagaagaa
     5401 gaatctttag atagagaatt accaggattt tttgcaagta tttttcctct attagcactc
     5461 gtaattatag ctataactgg aagtactttt ggttcagaat ttatgaaaaa aaatgtaatt
     5521 tacatagctt taatagttgc aattctttta tctgcttttt tattccataa atttctatca
     5581 tctaagatac agacattgag cataggtgct agtggttcta taggtcctat atttgctaca
     5641 tcttctgcag ttgcatttgg agcagtaata atggctgcac ctggttttga tgtatttagc
     5701 aaacttataa tgtcaatacc aggtagtcca cttataagtc ttactgtttt gacttcaaca
     5761 atgtcagcaa taacagggtc ctcttctggt gcattaggaa tagtaatgcc aaactttgca
     5821 gattattatt taagtactgg tctaaatcca gaattaattc accgtgttgc tacaatagcc
     5881 tcaaatatac taactatagt tccacaaagt ggtgtttttc ttacattctt aagcttatct
     5941 aagttaacac ataaaactgg atttaaagaa actttcattg tagttgcagt tggttcacta
     6001 atagcagaaa ttattgtaat tacattagga ttactaatgt attaatataa aacaaattaa
     6061 aattcataat atgaaaggtg gtgtctcaaa gtgaactttt tacttaatga gacactcttt
     6121 ttgtatgaaa tcaaatatat tttaatcatt tcaacaataa aaaagaacct ttctctattt
     6181 tgagacaaac cctttttatt ccttaaagat ttaaacaatt ataatttgtt actaatacga
     6241 aaacatctat tgaataaatt taaccaaata aaaaaaaatt tatcataact ataaatttac
     6301 gttatggaat tattataaaa atattatttt ctctaagtaa gtaattccat actatacagg
     6361 cttttaatac aaattttcaa acacactgaa atatacctat ttattccata tatatctttt
     6421 ttacatatct ttacagtaaa aaaagtctta atcttatgtt agtatagatt cacgggtacc
     6481 ccctacacta aaggttacaa atcagtaaca tttttagaat ttttagatta agaggtgttt
     6541 aatgatgaga agaaatatac caacaaaatt aaaaactata ttagctttag gagtgttaac
     6601 tttagcaaca gcttcaagtg ctgttactgc aagtgcttca tcacttgaaa ataatgtaaa
     6661 tataagtaac aaaagtttag tatatagtaa tactaaatac aagttttctg caaaaccaaa
     6721 ttgttctgta aaaccagaac ttaaacctga taaagataac tcttgtaagg ataagaatca
     6781 taatgacaag aattgcaaca acactaataa acctgaggac aacaataatt cagactctac
     6841 taataagcct gataacaata aaccagagtc taataaacct gaggacaaca ataattcagg
     6901 ctctactaat aaacctgata acaataaacc agagtctaat aaacctgaag acaacaataa
     6961 ctcaggctct actgataaac ctaataacaa taaaccagag tctaataaac ctgaagacaa
     7021 caataattca gactctacta acaaacctga tgataataac aactcaggct ctacaagtga
     7081 aaatttttca gcttaccaaa aagaagttgt cgacttagta aatatagaaa gagctaaagc
     7141 tggattaaat cctcttactc ttgattcttc tatatcaaat gttgctacaa aaaaatctca
     7201 agatatgatt gataacaact atttttcaca caattctcca acttatggtt ctccatttga
     7261 catgttgaaa aaatttggaa taagctataa gacagcaggt gaaaatatag ctatgggtca
     7321 aaaaactcct aaagaagttg taaacgcatg gatgaattct gaaggtcaca gaaaaaatat
     7381 aatgaatcca aatttcagta aaataggtgt tggagtagca caaaaaagtg gtggttcaat
     7441 ttattggaca caaatctttg taggataaaa tactcttttt ccatgcatat caaaagagta
     7501 tctctaagtg ataaaaaaca caaatgagat actcttttgt atctatttta aaattatcaa
     7561 atccaaatat acatactata aatattattt gttctcttaa ttttagtgtg tagaacaata
     7621 tataaaaagt attggcaaga tgaaaatgga aaacttaaaa agatatttaa taggtaaaaa
     7681 caggatctta atatcttcat aaaaaaaggc tgtctcaaaa tagagataag gcctttttca
     7741 ttgttgaaat gattaaaata tatttgattt catacaaaaa gagtgcctca tgaactaaaa
     7801 agctcacttt gagacactct cttttatcaa ttctatataa gcattataat actttaatat
     7861 tattcagcta attttttgta tccttcatat ctttctttag catcagcttc agctttagca
     7921 aataatccat cagcttcctc tgggaattgt ttagctatag cagaatatct tacttgtgca
     7981 agtatgaaat ctttgaagct tgcagttggt tcttttgaat ctaaagtgaa tgggttttta
     8041 ccttcagctt ttacttctgg attgaatctg tataaatgcc agtatccgca agcaacagct
     8101 tgtgcttcat tagccacagt tcttcccata ccttctttta atccatggtt tatacatgga
     8161 gcataagcta ttattagaga tggcccatca tgtttttcag cttctaaaac ggctttgata
     8221 aattgatttt tatcagcccc catagcaact tgagctacat atacgtttcc atatgtcata
     8281 gccatcattc ctaaatcttt ctttctagat tttttacctg aagcagcaaa cttagccata
     8341 gcagcagctg gagtagcttt agaagcttga cctcctgtat ttgaatatac ttctgtatca
     8401 aatacaagta cgtttacatc ttcaccagat gcaagaacat ggtctaatcc accgtaacca
     8461 atatcatatg cccaaccgtc tccacctagt atccattgag atctcttaac tagataatct
     8521 tttctatctt ttatagcttt tactaattct ttagcttttt catctgtaca atcacaagct
     8581 ggtttttcta ataattcaac aaccttagca cttgctactt tagaagcttc tccatcattt
     8641 ctagaatcta accactctgt aaatactgct ttagcatctt cacagatatc catagaaact
     8701 aactctgtca tagcatctac tattttattt cttatttgct taacagctgt gtacatacca
     8761 aatccatatt cagcattatc ctcgaataaa gaatttgccc aagatggacc tttaccttca
     8821 tggttacaag tataaggaat tgatggtgca gaagctcccc atatagaaga acatccagta
     8881 gcattagcta tcatcattct atctccatat aattgtgtaa ctactttaat gtacgcagtt
     8941 tctccacatc cagcacatgc tcctgaaaac tccattaatg gttgtctaaa ttgacttcct
     9001 ttaacagtat ttgcaggcat aacatcaact tttggagcta cttcatcagg atttactgca
     9061 aatgcccaat tatctaactc tgtatcaagt tgagtatcta ttggcttcat aactaaagct
     9121 ttttctttag ctggacatat gtcagcacag tttccacatc ctgtacagtc aagtggacta
     9181 acttgaattc tatattttaa tccttctaat cctttaccta atgctttttt agcttcaaat
     9241 ccttctggag cattagctaa ttcttcttca gtaactaata ctggtcttat acatgcatga
     9301 ggacatataa aagaacattg gttacattga atacagttat ctattatcca ttcaggaaca
     9361 tcaacagcta taccacgttt ttcataagct gcagtaccac aagggaatgt tccatcttct
     9421 ataccattga atgtacttac tggtaagttg tttccttctt gtgcagtcat tggtcttagt
     9481 atatttttta taaattctgg ttctttaact tctcctttat ctgcatcaac agcattttcc
     9541 caagaagctg gaacttcaac tttaactaaa gcatttattc ctgcatcaac agcagcatag
     9601 ttcatattaa ctattttttc acctttttta ccataagctt taactataga atcttttaag
     9661 tatttaacag catcttcttc tggtattatt tcagctaatt taaagaatgc agattgcatt
     9721 atcatgttta ttctgtttcc aagacctatt tcttgtccta gtttaacagc attaactgta
     9781 tagaatttta tatcattttt agctatatat tgtttcatat gagctggtaa atgttcttca
     9841 agtcctgctt ggtcccatat agtgttaagt acaaatgtac cacctttttt aagacctttt
     9901 aataaatcat attgataaac atatgattgt ttatgacagg caatataatc agcttcatct
     9961 attaaataag tagctcttat tggagtatca ccaaatctta agtgagacat agttatacca
    10021 ccagattttt tagagtcata atcaaagtaa gcttgagcat atttttcagt atggtcaccg
    10081 attattttga tagcttgttt attagctcca acagtaccgt cagaacctaa tccccagaac
    10141 ttacatctta tagttccttt agaagctatt tttattggct cagatggagt taaagaagta
    10201 ttagttacat catcaactat acctactgta aatccatttt taggttgttc tgaaactaag
    10261 ttatcaaaaa cagtttttat atctgaagga gtagtatctt ttgaacctaa tccgtatctt
    10321 ccacctatta tcataggagc attttctttt ccatagaata catcacgtac atctaagtat
    10381 aatggttcac cagttgaacc tggctcttta gttctatcaa gtacacatat tctttcaaca
    10441 gtagatggca tagcatctaa gaaatgtttc attgagaatg gtctatataa atgaacttta
    10501 actaaaccat atttttctcc cttagcattt aagtaatcta tagtttcttc tattgtttct
    10561 gttactgaac ccatagctat taatacgtat ttagcatctt ctgctccata gtaatcaaat
    10621 aaactatgtt ttctacctgt taaattactc attttttcca tgtatttttc aactatacct
    10681 actatatttt gatagtattt attagaagct tctctagttt ggaaatatat atctgggttt
    10741 tgagcagtac cacgagcaac tggatgattt ggagataaag cattatctct aaatgcttga
    10801 actgcttcaa agtttaataa acttgcataa tcttcatttt ctagtaattc aactttttgg
    10861 atttcatgtg aagttctgaa tccatcaaag aaatgtataa atggtaatct accttctatt
    10921 gcagctaaat gtgcaacagg tgctatatca gctacttctt gaactgaacc agaagctaat
    10981 aatacacatc cagtttgtct agcagccata acgtcttggt gatccccaaa tattgataac
    11041 gcttgagatg ccaaagcacg agcacttaca tggaatactc ctggtaataa ctctcctgca
    11101 actttataca tgttaggtat cattaataat aaaccttgag atgctgtaaa agtagaagtt
    11161 aaagctcctc cttgtaaaga accgtggaat gttcctgatg cacctgcttc tgactgcatt
    11221 tcaacaacat taactgtttg tccaaatata ttttttcttc cttgtgatgc ccactcatca
    11281 actacttcag ccatagttga agatggtgtg attggatata tagctgctac atctgtaaac
    11341 gcataggcaa catgagctgc agctgtattt ccatcaagtg ttttcataaa cttagccata
    11401 atatgtatcc tccttgatag ctacaattgt acttgtttat aacaagtcta tttttttata
    11461 tttacatata atttacacaa caaaaatata aacacacata tagtcgcgca aatactctat
    11521 cttctaatat aatactacag attagataaa attcctttga aaattgtaaa ttaattacat
    11581 atttaagtag atttttttta aaattaaata tgttaagttt ttttcataat tttgtataca
    11641 atatataata aattaagtta gactt
//
LOCUS       DAEPLF010000105        11560 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEPLF010000105 DAEPLF010000000
VERSION     DAEPLF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.105"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..248
                     /locus_tag="KR314_002311"
     CDS             <1..248
                     /locus_tag="KR314_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328692.1"
                     /translation="VFEYHFLTNLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNEL
                     FELASKVSENYDYQRLLDVYNYIFKEIETKEVIKLIF"
     gene            274..846
                     /gene="folE"
                     /locus_tag="KR314_002312"
     CDS             274..846
                     /gene="folE"
                     /locus_tag="KR314_002312"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBH0328693.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            912..1703
                     /gene="folP"
                     /locus_tag="KR314_002313"
     CDS             912..1703
                     /gene="folP"
                     /locus_tag="KR314_002313"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBH0328694.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELEIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            1714..2079
                     /gene="folB"
                     /locus_tag="KR314_002314"
     CDS             1714..2079
                     /gene="folB"
                     /locus_tag="KR314_002314"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBH0328695.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            2072..2578
                     /gene="folK"
                     /locus_tag="KR314_002315"
     CDS             2072..2578
                     /gene="folK"
                     /locus_tag="KR314_002315"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBH0328696.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDILFFN
                     DVASTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTVKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            2559..3344
                     /gene="aroF"
                     /locus_tag="KR314_002316"
     CDS             2559..3344
                     /gene="aroF"
                     /locus_tag="KR314_002316"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBH0328697.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLKILKAVKDEVGMAVITELMDVRD
                     IDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            3599..5389
                     /locus_tag="KR314_002317"
     CDS             3599..5389
                     /locus_tag="KR314_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBH0328698.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLDCGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            5407..6573
                     /gene="rpoD"
                     /locus_tag="KR314_002318"
     CDS             5407..6573
                     /gene="rpoD"
                     /locus_tag="KR314_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBH0328699.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            6738..7427
                     /locus_tag="KR314_002319"
     CDS             6738..7427
                     /locus_tag="KR314_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH0328700.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            7441..8538
                     /locus_tag="KR314_002320"
     CDS             7441..8538
                     /locus_tag="KR314_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBH0328701.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            8600..9172
                     /locus_tag="KR314_002321"
     CDS             8600..9172
                     /locus_tag="KR314_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBH0328702.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEVLEEMNIEVDIFRVSDEVAKT
                     LPQYYLINYKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(9232..9699)
                     /locus_tag="KR314_002322"
     CDS             complement(9232..9699)
                     /locus_tag="KR314_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBH0328703.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(10085..10561)
                     /locus_tag="KR314_002323"
     CDS             complement(10085..10561)
                     /locus_tag="KR314_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBH0328704.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLRVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(10681..>11560)
                     /locus_tag="KR314_002324"
     CDS             complement(10681..>11560)
                     /locus_tag="KR314_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBH0328705.1"
                     /translation="ILVFTFNKLELSYFIGIIGFLLYESILYIHTHKKAKKLKSEIYS
                     KLGHIDVDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIFIAFNYNKLPDSIA
                     THWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSRIKIDTNRIEESRV
                     ENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLLVIIYLIATYINSP
                     NLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINFGNPLGKILYIAIA
                     LLLIFSLFSLIKSLLL"
BASE COUNT         4646 a         1255 c         2085 g         3574 t
ORIGIN      
        1 atgtttttga atatcatttt ttgacgaatt tatatttaaa agatataata attgggtact
       61 ttaatataaa tgataaatgg gtaccaaaag acaaaaaatt gctaaaagtt ttaaaaaaag
      121 aaaataatga gctttttgaa ctagcgtcaa aagtaagtga aaattatgac tatcaaagac
      181 ttttagatgt ttataattat atttttaaag aaattgagac aaaagaggtt ataaaactaa
      241 tcttctagtc acaaatcaat ggggggagtt taaatgaata aagttgataa agaaaagata
      301 caacatgcag taagagaaat actagaggca attggagaag acccagatag agaaggtcta
      361 atcgagactc caaatagagt tgctaggatg tatgaagaga tattctctgg tcttagtgaa
      421 gaaccaagag accatttaaa agttcttttt gcagatgaaa aacatgaaga attagtattg
      481 gtaaaagata ttccatttta ttcatgttgt gagcatcatc tagttccttt ctttggaaaa
      541 gctcatattg cttatttacc aaaaggagga agacttacag ggctttctaa acttgctaga
      601 gtaatagata cattggcaaa aagaccacaa ttacaagaaa gaatcactaa aaatgctgca
      661 gatataataa tggaagaatt acaaccttat ggtgtcttag tagtagtaga agctgaacat
      721 atgtgtatga caatgagagg tgtaaaaaaa ccaggttcaa aaactgttac atcagcagtg
      781 agaggaatat ttgaaaagga cattgcgtct agagccgagg ctatgagttt gattactatg
      841 aagtagcaat ttattataga tataatcatt tattgagata taaaatttaa tttaagggag
      901 tgtcattaat tgtgtttgat tatggtaaaa gaacttatat aatgggaata ttaaatgtaa
      961 ctccagatag tttttctgat ggaggagatt ttaataactt ggacatagca atacaacatg
     1021 caaaagatat ggttgaccaa ggtgcagata taatagactt aggaggagag tctacacgcc
     1081 caggtcatag ctatgtagac tcagacgaag agctaagaag agtaatccct gtaataaaaa
     1141 aacttaaaca ggaattggag ataccaatat caatagacac ttataaggca gatgttgcag
     1201 aagaagcatt aaaattaggt gtcactatgg taaatgatgt ttggggtctt agaaaagata
     1261 aaaatatggc ttctgtaata ggaaaatatg atgcagaagt gtgtataatg cataatcaag
     1321 atgggacaaa ctatgacaaa gatataatgg agtcaataaa agactttttt aaggtaagta
     1381 tagaaatggc aatgagttgt ggtgtaaaaa aagaaaaaat agtacttgac ccaggcgtag
     1441 gatttggcaa ggattttgaa caaaatattg aagtattgag aagattaaat gaattaaaag
     1501 atttagggta tcctattttg cttgggacat ccagaaaatc agtaatagga aaagtacttc
     1561 cagttgagcc taaaaaaaga ttagaaggta caatagctac tactgttctt ggtataagag
     1621 atggcgtaga tatagttaga gtacatgatg tttatgaaaa tttaatggca gctagaatga
     1681 ctgatgcaat atatagaaag taggtataat ataatggata aaatattact tagtaatcta
     1741 ggattctatg gatatcatgg tgttcttaaa gaagaaaatt ttctaggtca gaaattcttt
     1801 gtcgacatgg aactttatat tgattcaaga gaagcaggtc ttagtgacga tataaataaa
     1861 tctgtaagct atgccgaggt ttacaatgtt gttaaagata ttactgaaaa taaacaattt
     1921 aatcttttgg aggctttagc ggaaaatatt gctgaagaag tcttgaataa atttatactt
     1981 ataaatggag ttatggtcag agttagaaag cctgaggctc ctgtaaatgg tatttatgac
     2041 tattttggtg ttgagataag gagaacaaga gatgaataag gcttatttgg gaataggtac
     2101 taacatgggt gatagatttg ataatttatc aagagcatgt gaacttttga aaaatagtga
     2161 ttctatatat aaagtaaagg aatcaagttt atatgaaaca aaaccatggg gatatacaga
     2221 acaagcagat tttctaaata tgtgtgtaga aatagaaaca gaatttgaac cttatgagtt
     2281 gttagaatat tgtcaagaaa tagagagaga actacacaga gagagaatag ttcattgggg
     2341 accaagaact atagatgtag acatactatt ttttaatgat gtagcttcca ctgatgaaag
     2401 actaacaata cctcatccga ggattcaaga tagagctttc gttttgatac cacttatgga
     2461 tttaaatgaa gaacttataa taaatgaaaa aacagtaaaa gaacatttaa acctcctatc
     2521 tgctgaagaa agagaagaag taaaggagct tgtaggttat gaaagaaaac ctatttaata
     2581 ataattcaaa gatagagata aatacaaata acaaaaagat aactgtaaaa aaagataaac
     2641 ttataatagc tggaccatgt gcaatagaga gttatgagca acttttggag actgctaaat
     2701 ttgtaaaaag tcagggagca aatattttaa gaggaggagc gtacaagcca agaacatcgc
     2761 caaattcatt tcaaggatta aaaaaagaag gtcttaaaat attaaaagca gttaaagacg
     2821 aagttggtat ggctgttatt acagaactta tggatgtaag agatatcgac gaactgtata
     2881 gtataagtga tataattcaa attggttcaa gaaatatgca aaactttact cttttaagtg
     2941 aagtgggaaa acaaaataaa cctgttatgt taaaaagagg aatagcatcc acaattactg
     3001 aatggatagg tgcttctgaa tatatagcaa tagaaggaaa tagtaacatt ataatgtgtg
     3061 agagaggtat aagaacgtac aatgattata ctagaaatac attagattta gctgcggtac
     3121 ccattattca aaaggaaaca ggtcttcctg ttgtggtaga cccaagtcat gccactggag
     3181 ttagatattt ggtaaaacct atgtcactgg catcttttgc atgtggagca gatggaataa
     3241 tggtagaagt acatcctgac ccagaaaatg ctttgtcaga tggtgctcaa tctctttgtt
     3301 ttaatgaatt tgaagattta atgaaatcaa ttaataatta ttaaaagagg caagagcctc
     3361 ttttttattc gtaatcattg ctaaggtaaa tttataagtt ataatttgat agttagatta
     3421 tttaatataa ataaagattt aatattgata gaattaatat aaaaaataaa aattttttga
     3481 ttaataagaa ggatatcaat aaaaaaagtc gaattgtgta tattgagtga ttgacaaatt
     3541 tagaatatag tgtagaatag aaatgttaac acaaaaaatt acaccagaag gtgataatat
     3601 gaatgatata aaagacatta ttgaagaaat aaaagctaga tgcgatatag caagtataat
     3661 atctgaatat atgaatataa aacaatctgg agctaattac aaagggttat gtccatttca
     3721 tggtgaaaag acaccttctt tttatataaa tacatcaaaa cagatttata agtgttttgg
     3781 ttgtggtgaa ggtggagacg taataaattt tgtcatgaaa atggaaaatt tggattttat
     3841 ggatgctgta aaaattttag caaataaatg cggtatagaa attaacacta acatgaatga
     3901 agaaactagg attaaaatag aaaaatctaa gaaatttcaa gatatccata cggaagcagc
     3961 tagattttat ctttcaaact tattgggaag caaaaatctt gggtatgaat atttaagaat
     4021 aagaggacta gatgataaaa ttattaagaa atttggttta ggattttctt tggattcatg
     4081 gaattcactt atgaatactc taataagtaa aggatacaaa aaacaagact tattggattg
     4141 cggtctaatt gcaaaaaata gagatggaac taattgctat gataaattta gaaatagagt
     4201 tatgtttcct atatttgact atagaggaaa cataatagga tttgggggaa gagtattaga
     4261 tgattctttg ccaaaatact taaattctcc agacacatta atttttaata aaaaacagaa
     4321 cttatacgga ttaaattttg ctagaaaaaa tttagaaagt aaaactatag tcttagttga
     4381 aggatatatg gatttaattt cgctttatca gtatggtata aaaaatgtag tagcaaccct
     4441 tggaacagcc ttgactgaac aacaaggcat tttaataaag agatatgcgg acactgctat
     4501 tatatcttat gattctgatg aagcaggaat aaaagcgact ttaagagcta tagatatact
     4561 tactaaatta ggtatcaatg taaaagtgtt agacttaaaa gatgctaaag acccagatga
     4621 atttgtaaga aaatacggac ttagtgatta taaaaaagct atggatgttt ctactcacta
     4681 cataaaatat aaaatagatc atcttaaaaa agaatttaac attcaaaaag atgaagaacg
     4741 agtgaagttc gcaaaagaag cttctaaaat tataaaacaa ttgacaagtc ctgttgaaat
     4801 tgatttttat acaaagtatt taagcaatca aatagatatt aatgtcgagt ctataaagag
     4861 agaagtttat ggcaaaaatt acaataagcc atataataac aaaaatcaaa agaaaataga
     4921 agaaaaagta attgaaaaag tcgaagtcag acaagacgga aaacaattag ttgaagaaac
     4981 tctaataaaa ataatgttag aagacaaaaa aattagagaa atagcgttac ttaaagtaga
     5041 agaaagtgat tttttattga aagaaagtaa agaaatttta aattacatga ttaaaaatca
     5101 agaattggac aaaataacta ttgacaaatt gaaaagttta aacatatccg aagaatactt
     5161 aaaagaatta aattcaattt ctttgaatag tataaactta gagaacacaa aagaaataga
     5221 aggaataata acaaatatca gaaaaaactc attagaagag caaattaata gcttgttgag
     5281 agaacaacaa gaattagaaa ataataatga tatgaaagag gtagatggta gagttatgga
     5341 aattgcttta aaaatagttg agataaataa aattttaaag agcttgtaga gaaaggaggt
     5401 ttgagtgtgg aaaataaatc gaataaaaaa gagttaaaaa aggttactgc taagacatta
     5461 atagaaaaag gaaaaaagca aggttctctg acacttgcag agataatgga agctttttcg
     5521 gagactgaac ttgataagga tcaagtagaa aatctttatg agactttagg aaatttggga
     5581 atagaaataa cagaaacaaa aaattataaa gctgatatag atttttcggt tgctgatgac
     5641 gatttaagta taggccactt agatgaagat gcagaggcaa tttcacatga tgactcttct
     5701 gcaatagaaa tagaaactgt ggatttatct ttaccaaaag ggataagtat agatgaccct
     5761 gtaagaatgt acttaaaaga aataggaaaa attcctctac ttaaaccaca tgaagaagta
     5821 gaatttgcta gaaggatgca cgaaggtgat gagatagcaa aacaaaggtt agttgaagca
     5881 aacttaagac tagttgtaag tatagcaaaa agatatgtag gaagaggtat gcttttcttg
     5941 gatttaatac aagagggaaa tttaggtctt ataaaagcag tcgaaaaatt tgactataca
     6001 aaaggatata agtttagtac ttatgcaaca tggtggataa gacaagctat aacacgtgct
     6061 attgcagacc aagctagaac tataagaata ccagttcata tggtagagac tataaataag
     6121 ctaataagag tatcaagaca actacttcaa gaacttggaa gagatccaaa accagaagaa
     6181 attgcaaaag aaatggaaat gacagaagat aaagtaagag aaataatgaa aatagctcaa
     6241 gaccctgtgt ctttagaaac accaatagga gaagaagaag atagccattt aggtgatttt
     6301 attccagatg atgatgctcc agccccagca gaggcagcag catattcttt attaaaagaa
     6361 caaatagaag atgtacttgg ttcgttaaat gatagagaac aaaaagtatt aaagcttaga
     6421 tttggtcttg aggatggtag agccagaact cttgaggaag ttggaaaaga gtttgatgta
     6481 actagagaga gaataagaca gatagaagca aaagcactaa gaaaactaag acatccaagt
     6541 agatcaaaaa aacttagaga ttatttagac taaaaagata catcattgat gtttttgtga
     6601 taaatgccat gagataagtg atatctcatg gctattttgt agtaaagatt taattttatt
     6661 tttgtagtta tttcagtcta atatctataa cttggattaa atcctataaa taaagggaat
     6721 tgaaaggaga attatatttg aaattaacag atagattatt gaaaatagca tcattagtgt
     6781 cagatggtaa aaaaatagct gatataggga cggaccacgg atacatacca gtatatttgt
     6841 taaaagaggg aaaagtacca tttgcagtgc tagcagatgt taataaagga cctcttgaca
     6901 atgcacataa agaagtcata cagaacaatc ttttagataa agttgattta cgattgggtt
     6961 ctggtataga gatacttgaa ataggggaag tagaagaagt gataattgct ggtatgggtg
     7021 gaatcttgat aagtgagctt ctcgaggcta aaaaagaagt agcacataat gtagaaaaat
     7081 taatattaca gccaatgcaa gcacaagaag aacttagata ttacctttta aacaacggat
     7141 atgaaatttt agaagaagtt ttagtgagag aagattttag aatatatgag ataattgttg
     7201 ccaaatatac aggtaaaaat actataatag aagatgagat atattatgag gttggaataa
     7261 agcttttaga aaacaaagat tctcttttta atgattttat agaaaaaaag ataaagacat
     7321 atagttctat agttaataaa ttagaaggga aaaatgggga agctatagat aagaagagaa
     7381 aggaaagcga agttgcaata aaaaaacttg aaaatttaat aaaataaata ggaggtatat
     7441 atgctattaa agagtctaac tagaaaaatt gaaaaaaagt atcctctaaa cttagcagag
     7501 gattgggata atgtaggtct tatagttgga gattttgata tggatgtaaa aaaggtattg
     7561 gtatctttag aagctaatga agatgttatt gatgaagcta tatccaaaaa tatagattta
     7621 attgtgacac atcatccttt tatatttggg aaaattaata aaattaatag tggagattta
     7681 aaaggcagac ttatccaaaa acttattaaa aatgatatat cactttattc tatgcatacg
     7741 aattttgata tagcatttga tggtctaaat gactatttta tggaaattat ggaatttggt
     7801 aattcaaagg ttttagatat aactaaaagc gaaaatttgt ataaattagc agtatacgtt
     7861 ccacataact attcagatga acttagaaag gttttaagta attcaggagc aggtcatata
     7921 gggaattata gtgattgtac gttttcaata gaaggtgaag gacaatttaa gccattagaa
     7981 ggctctaatc cgtttcttgg aagtgtaaat gatatagaaa ctgtaaatga agttaaaatt
     8041 gaaactgtag taccacagaa gcttttaggt ggggtaataa gttctatgtt agatgcacat
     8101 ccttatgaag aagttgcata tgatttgtac aagttagaaa ataaaggtga gatatttgga
     8161 ctaggtagaa taagtaaact tgataaaagt atgacattag aaagtctttc aaggaaaata
     8221 aaagagaaat taaatatgaa acacataaga gtagttggaa accttagtac agacataacg
     8281 aaagtagccg ttgtaacagg agctggttca gaatttgtaa aaaaagctaa gagacaaggt
     8341 gcagaagtac taattacggg tgatgtaaag tatcatgagg cacaagatgc acttgatatt
     8401 ggaatgtgta tagtggattg tggtcatttt gatacagaag atatattcaa aaatgttatg
     8461 aagagatttt tagatgaatt tagtgaaatt gaagtaataa agagtaatgt atatttgaat
     8521 ccatttagta ctatttagaa gtgtttgatt aaaaaatact atataaaaaa taaatttgag
     8581 tttaattagg gggataatga tgaaagtagc agtaattttt catagtgttt gtgggagtac
     8641 atatttatta gctagagaat ataaagaagt gcttgaagaa atgaatatcg aggtagatat
     8701 atttagagta agtgacgaag ttgcaaaaac acttcctcaa tattatttga taaattacaa
     8761 agaatacaaa gacgaatttg aaagtataaa tgtaattaaa tctggaaaag aaatattaga
     8821 ttatgatgct atatttatgg gttcaccaac atattatgga aatgtatctg gaccaatgaa
     8881 gatgtttatg gatagttttt ctgatatttg ggtaggtgct cctttgagtg gtaaaatatt
     8941 tggttgtttt gctacagcag gttcacaaca tggaggagga gaattagctt tgcaagctat
     9001 gaatatattt gctcaacata tgggaatgac tcttttgtca gttccatgtt cagtaagagg
     9061 aggttatcca gcgtatggaa tacttcatat agcaggagat aattctgata taagaccaaa
     9121 tgatgatgcc aagataggta taagagatta cctaaaaaga cttaacatat aagaatatca
     9181 tataaaaata cctaaaagta gctgtatata cttttaggta tttttactta attataaacg
     9241 tgaagcttta ccagaaatat ggtcaatttc tattttaaca acagttgtta agttcatatc
     9301 cttctctatg tacttcatgc cttcttcaaa gaatccttta gaatattttt ttattatttc
     9361 tacaagagca tttttctttt cttcattttc tacagtgcaa gcttttccaa aaactatagc
     9421 actagaataa gtagtactaa atttatctgg aataacactt tcattagcaa ctactaaaaa
     9481 agaaactttg ttgtttttcg atatattatc taacttatgt ccatttcttg cacaatgaaa
     9541 gtaaatagaa tcattaaagt atacataatt tacagcaact ccataaggat aaccattttc
     9601 agatatagtg gaaaaagtac caaattcacc attttttaat acttcaacag tatcttcttt
     9661 tgtcatttct ctttttttca atctcatttc tttaaacata atatcatcct ttctaatttt
     9721 gatgcttaaa ttaaatttta gcacatttat aaagattatg ctatgttttt tacaaatttt
     9781 aagaaaaaaa tatagtttaa gataaaaggt agtgaaagag aagctatttg gaggtttatt
     9841 tatgatatta ttgttagagt agtacaaaca atactaataa atttaatagc tggtttcttg
     9901 ttatttaagg tagtaagcat gtctataaga aggggaagtt atcgagggta gaagttgtta
     9961 tttataacga tattataaag aaaatattat tatataaaat aataaatagt aaagaggtat
    10021 caagttgaat ataatactta atacctcttc aaaattaaat tataaaagtg ttgtgggaat
    10081 tgtcctatac atggtaagga gataataaag aatgtatctt taaagtctca actaaacagc
    10141 aaaatttatc caacaaacat acaataaatg cttctttgta tctaggtggc ttaacagtaa
    10201 gtggccacat gtgttttaaa attatgtccg tttctttttc atttatttga aaaaataaag
    10261 ttgcattttt taatgcttct ctagggtgag taaatccatg taatcctttt ctatcacctt
    10321 tgtaatgcca atcataaaga aaaaagtcgt gtagtaaagc tcctctaata acactttgaa
    10381 tatcaacacg taagtgcaat tttttacata atagataact ataatatgct actgacaaac
    10441 tatgttcaag acatgaaaca ttgccatgat gaggaaatgt tctcataagt tgaacttttt
    10501 cagaagaaag tatctcagaa gcacattcat taaaatattt gatttcagct tttgacaaca
    10561 ttatgaaaat ctccattctt agtaatatat aaatactata cttcaacgat aatatgaaat
    10621 atttcttatc ctacaaaata taatacccaa taatacaagt aagatacctt ttatggtatt
    10681 ttaaagtaag agagatttta ttaggctaaa caatgaaaaa attaaaagta aagctatagc
    10741 aatatatagt atttttccaa gaggattgcc aaaatttata gtccatccaa taccaaacct
    10801 tttattaacc attaatgatg ggtcatttgg attattatac atgatgcctg ctatccaata
    10861 cttctcatct tcttctggag aataagatga gttgaatttt aaatttgggg aattaatata
    10921 tgttgctatt aaataaatta taactaaaag cgttgtaata ttcatagcag ttgatagttt
    10981 tgtttttatt gataaaagag tagtaaaaaa ctgtgttgtc attataatca tcaatattaa
    11041 aaacaaataa ccaattttat ttaaatattt aatgttttca actctacttt cttcaattct
    11101 atttgtatct atcttaatcc ttgactttat ggatccaatg gaagttatat ataataaaac
    11161 cattaaacaa aagtcagtac cgattaattt aaatacatta agaaatgatt tatctataaa
    11221 aacatcagga gacccattta tgttccaatg agtggcaatt gagtctggca atttattata
    11281 attaaatgct ataaaaatac tcatccccaa agtaaatagt atcggtatta aatagattat
    11341 tttaaatttt tttattattt tatttttctt atttataaaa tccatatcta ctattaattt
    11401 tgaatctaca tctatatgac ctaatttaga ataaatttct gatttaagct tcttagcttt
    11461 tttatgtgta tgaatatata gtatactctc atataataaa aaacctataa taccaataaa
    11521 atatgaaagt tccaatttat taaatgtaaa aaccagaata
//
LOCUS       DAEPLF010000106        11519 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEPLF010000106 DAEPLF010000000
VERSION     DAEPLF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.106"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..420
                     /locus_tag="KR314_002325"
     CDS             <1..420
                     /locus_tag="KR314_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBH0328706.1"
                     /translation="YSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVS
                     YVHIMTLQVFLYMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYF
                     ILIVSGLFTFGTHYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            569..730
                     /locus_tag="KR314_002326"
     CDS             569..730
                     /locus_tag="KR314_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBH0328707.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            1071..2339
                     /locus_tag="KR314_002327"
     CDS             1071..2339
                     /locus_tag="KR314_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBH0328708.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            2534..3322
                     /locus_tag="KR314_002328"
     CDS             2534..3322
                     /locus_tag="KR314_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBH0328709.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            3322..3771
                     /locus_tag="KR314_002329"
     CDS             3322..3771
                     /locus_tag="KR314_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBH0328710.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            3775..6039
                     /locus_tag="KR314_002330"
     CDS             3775..6039
                     /locus_tag="KR314_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0328711.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKNALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            6179..6490
                     /locus_tag="KR314_002331"
     CDS             6179..6490
                     /locus_tag="KR314_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328712.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            6776..7276
                     /locus_tag="KR314_002332"
     CDS             6776..7276
                     /locus_tag="KR314_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBH0328713.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIISGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            7333..8319
                     /locus_tag="KR314_002333"
     CDS             7333..8319
                     /locus_tag="KR314_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBH0328714.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            8369..9091
                     /locus_tag="KR314_002334"
     CDS             8369..9091
                     /locus_tag="KR314_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBH0328715.1"
                     /translation="MENFNKNAKIIGIGVEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            9120..11054
                     /locus_tag="KR314_002335"
     CDS             9120..11054
                     /locus_tag="KR314_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0328716.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFIEESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALDGGYFFPVRWIEYGKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            11047..>11519
                     /locus_tag="KR314_002336"
     CDS             11047..>11519
                     /locus_tag="KR314_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328717.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKIRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLST"
BASE COUNT         4601 a         1051 c         2079 g         3788 t
ORIGIN      
        1 tattctataa aaatatttat taaaaatgta agaatcttag ttttaagtat attacttact
       61 gtgttacaat taatcgcata tttcagtata gtttattgtg tgtataaagc ctcaaattta
      121 agaggagttt catatgtaca tataatgaca ttacaggtct ttttatatat ggctatatca
      181 ccaattccaa caccaggaaa tgttggagct aatgaggtta cattttttac tatgttctcc
      241 aatataattc caaaagaatt attggggtac tctgtactct tatatagtgg gtttgtatac
      301 tattttatat taatagtttc aggtttattt acatttggaa cgcattatac attaaattct
      361 tggaaaaagg aagataaagt aaattctaat ttagataagg ttattgaaaa attaaattaa
      421 taaaataatt agggaaattc tatatataaa tatggtgttg agtaagctat attatattga
      481 gatatttccc ttttttgatt aaaattatag aaatttgaaa taataagtaa taaacagaaa
      541 aagtgatatt gttttgaaag gagaaactat gaaacaagat aaacaaaatg aaaaacaaag
      601 tgagatttct aagataaaac aaagcaatga taataaactt gaaatagctc aagaattaca
      661 tgtaagtaaa aatataggga atatgcttgg atatagaata gataagaaaa aaatgaatag
      721 accaagttaa tgaaaatgtt gaaaatggta cattttaaaa aatatatata atgaaatgtt
      781 ttattgttaa atttaaactt attttaatgg aggaaaaagt cgaaaaatat ctaaaaagat
      841 atggatataa aaaatattaa tatatatata ctataaactt gacaaaatca accttttaat
      901 aactagcaaa aaatagcaaa attatataag cgttatttaa ttatcaaaaa tgaaagctaa
      961 attgcatgtc ctaaaaaaaa tgattgataa catttatttg tgtgctataa ttatatagta
     1021 aaacaataag tatattgcat aatgtatgca aatcgggagg agagacggac atggaagcat
     1081 ctatacacac aattgccagc aataatttat tgataatttt tgctattgta agtataactg
     1141 gaataatttg tagtaagctt agtgaaataa taaaggttcc agatgtagtg ttatatttac
     1201 ttgtaggtat tttaatagga ccatcatttt taaaatttat agatattcgt ggatttcaaa
     1261 tagaaaatca actaatactt acatttggtt ctgcatttat tttatattta ggtggaaaag
     1321 aaataagttt aaaagtattg agaaatgtga agatatctgt attcttatta gctactttag
     1381 gagttgtgat atctgctttt ataatgcaac aagtaatagg atttactttt gggataagtg
     1441 caatgactgc tttactagca ggaactataa tagcatcaac agacccagcc actcttgtac
     1501 ctatatttaa tcaggtaaag ataaaggata gggtaaaaca aactgtaata agtgaatcag
     1561 cttttaatga tgcaacagga gctatactaa catcagcagt gttggctgta attttgtcaa
     1621 ataaattttc attaggtgaa aatatttatg aactggggac aatggttatt gtcggagttt
     1681 tagttggttt aattacagga atactgcttt tgaaattagt taatgataaa ccttatggaa
     1741 tatttaaaga ttttgcacca ataatatctg ttatttcagt tgtagttgca tatgaattat
     1801 ctacaaaatt aggtggaagt ggatatatgt cttgctttat tgtaggtata ataactggta
     1861 acaagaaaaa ctttaaaatt tggttaagtc aaaaatctta tgatgcagat ttttatgttg
     1921 ctgaaacttt aggtactatt tgtcgtatgg caatatttat aatattgggt agccaagtta
     1981 atttggctca gttaagtaaa tattttatgc catctgtaat tacagtatta gtattaatgt
     2041 ttatagcaag accactttgc gttttagttt gtacacttgt agacagggaa gcaaaatgga
     2101 gtaaaagtga aatattattt atgatgtggg taagggaaac aggcgtaata ccagcagcac
     2161 tttgtggtat tatttcagca atgaaagttc ctggatacga agtgatttca tctgtagtat
     2221 ttatgactat attaattaca ttagtaatac aaggaagtac tacaaaatta gtagctaaaa
     2281 aacttggatt attagaagaa gaagtgataa atataagtga aaaagtatca actttttaaa
     2341 gttttgcttt aaaatagtac ctgttaatgg ctattttaat attgtctaat gatgattatt
     2401 gaaatagtca tttttttatg tataagtaat ttacagaatt tacatattag acatatttat
     2461 tatcaaaatt tgttgaagct atgatataat tagttgtatt taaaataaga atagcaaata
     2521 agggaggaaa tttatgttta cggttatgga tattgtacag ccagatacag tagaagaagc
     2581 gtactcaatt ttaaataaaa gaaaaactaa ccaagtaatt ggtggaagtg cttttttaag
     2641 aatggggaaa aaaagaatag gaacaggaat agaattatct aaattaaatt tagattatat
     2701 aaaagagtat gaagattacg ttgagattgg ttctatgact acttttagaa cattagaaac
     2761 aagttccata ataaaaaata attttggaag aataatagaa gattcagtta aaaatataat
     2821 aggagttcag tttagaaatg tagtaacagt tggtgcaaca gtattttcta aatatggttt
     2881 ttctgacttg atagtagcat tactttcatt agatacggaa gtagaattat ataatattgg
     2941 gagaattagt cttgaagagt ttctaaatag agattatgag aaagatttac ttattaagat
     3001 atacataaag aagacaaata agaatgcttc ttataaatca cttagaaatg caaagagtga
     3061 ttatccaatt ctaaatgtat ctgtatcaaa acatatggaa caatttaagt tatgtgtagg
     3121 agcaagaccg cagaaagcta cgattgcaaa acaagctagt gaatttctat ctaacaatga
     3181 aatcaatgaa attaatatag ataaagcagt agaaatagca tcagaagaac taacttttgg
     3241 ttctaacatg agagcatcta gggagtatag aaaagctatg agtaaagtac ttttaaaaag
     3301 agctataatg gaggttattt aatgttagta aagattaata taaatgggaa gaaaaaaagt
     3361 gttgatatag aaccagaaga atatctggtt gatactttaa gaaaagttgg taatttaagt
     3421 gtgaaaagag gttgtgacac aggctgttgt ggtttatgta cagttttaat tgacaagaaa
     3481 cctacattat cctgtgcaac actgacattg agagctttag ataaagaaat aactacaatt
     3541 gaaggtttag aaaatgaagt taaagaattt agtgaagttt tggtaaaaga aggagctgag
     3601 caatgtggtt tctgttctcc tggctttata cttactgtac ttgcaatgaa ggatgagctt
     3661 gaaaatccta cagatgatga aataaagcat tatcttacag gaaatctatg tagatgtaca
     3721 gggtatatgg gacagttaag agctatcaaa acttatctgg aggtaaaata gaacatgaat
     3781 atagttggaa aaggaatcaa gaaaatagat ggaatgagta tcacaacagg aaagcctgta
     3841 tatacagatg atttatcagc aaaaaatgct ttggtggtaa agatacttag aagtcctcac
     3901 gcttatgcaa aaataaaaga tatagataca tcaaaggcag aaatggttga tggtgttgaa
     3961 tgtgttttaa catataaaga tgtaccaaaa tctcgattta cacttgctgg tcaaacatat
     4021 ccagagccat ctccatatga tagattaatt cttgaagatg ttgtaagata tgtaggtgac
     4081 gaagtagcta tagtggcagc tatagatgaa aaaactgcaa ttaaagcaat gaatcttatc
     4141 aaagtaaagt atgaagtact tacacctgtt ttggattttg aagaggcaat aggaaataaa
     4201 actatggtac atgatgaaga tagacattgt aactttgata taggtatgca aagagaaaaa
     4261 aatatagtat cccatcatga ttatacaaaa ggtgatgtag aaaaagtatt aagtaattgt
     4321 gatgtagtga ttgaagaaac ttattatact caagctcaag cacatgcaat gatggagacg
     4381 tatagggcat acaattattt agaccatatg ggaagattgg ttgtagtaag ttcaactcaa
     4441 ataccatttc atgtaaaaag acatttatct cgtgcactag aaataccagc tagcaaaatt
     4501 agggttataa aacctagaat tggtggcgga tttggtggta aacaaactgc gtgtgttgag
     4561 attttttctg caattgttac tttaaaaaca gggaaaccag ctaaaataat ttatgataga
     4621 aaagaaactt ttaattgttc aacaagtaga catgcaatga gattaaaagt tagactaggt
     4681 gctacaaatg atggcataat aaaggcaata gatataaaag cactttctga tacaggagca
     4741 tatggagagc atgcttctac aactattggt ttggttggag aaaaaacttt accaatgtat
     4801 aataaagttg aagcaacaag atttattgca gatgtggtat atactaataa gatgccagca
     4861 ggagcactta gagggtatgg agcatcacaa ggatgttttg ctgttgaatc aactataaat
     4921 atgcttgcta ataaattaaa aatggaccca acagatttaa ggttaaaaaa tgtggtaaca
     4981 gaaggtgaaa ctacatttgc atatggtaaa actttaaata gtgtcgattt aataaaatgt
     5041 ataaataaag gcaaagaact tattggatgg aatgaaaaat atccttgtaa acatcttgat
     5101 aatggaaaaa ttagaagtgt gggtatggca attactatgc aaggttctgg tatacaaggt
     5161 cttgataagt cttcagctga aataaaattg aatgactgtg gagattatac tcttatgata
     5221 ggttctactg atatgggtac aggaagtgat acgattttag ctcaaatggc tgctgaggtt
     5281 cttgatacaa gcattgaaaa tataacagtt atatcagctg atactgactt agttccatat
     5341 gacccaggtt catatgcttc aagtactact tatgtaacag gtatggcagt agttaaggca
     5401 gctcaagact taagagaaaa aataatttct gctggagcaa ctaaactagg tgttgagaag
     5461 gatgaagtag aatttgatgg tataaaatta actagtaaaa aaggtagcac tttattattc
     5521 gatatagcag ttgattctac aattggtcca gatactaatc aattaatagg tcttgcttct
     5581 catggtagcc cagtatctcc tccacctttt atagcaggtt ttgcagaaac tgaaattgac
     5641 acagaaactg gaaaagttga tttagtaaat tatgtagctg tagtagattg tggaacggtt
     5701 ataaataaaa atctagctaa gatacaagtt gaaggtggaa ttgttcaagg gattggaatg
     5761 gcattatttg aagaagttag atacacagaa aaaggaagca tggacacaaa tacatttatg
     5821 caatacaaga ttccagctag atgtgatatt ggaaatgtta aagtagattt tgttgaaact
     5881 tatgaaccaa ctggaccatt tggtgctaaa tctgttggag aagttgttat aaatacacct
     5941 ttaccagcaa tacaagaagc tgtgtttaat ggaattggtg taagaataaa tacactccct
     6001 attacacctg aaaaagtttt tatggaaatg aataaataat tatttatgaa aatttagact
     6061 agttttacat gttaaacaac cagttatgtc atacctgttg aaagatattt aataagtagt
     6121 tattataata ttatgaattt tacttataaa atacaaaatt aaataagggg atgatgtttt
     6181 ggctggtaat ctaaataata tgagagcagt aaataatttt agaggagata agaacatttt
     6241 agaatgttta gtcagctttg agggtcgttc aataagtcag agaaaagtaa gggtattttt
     6301 taaagaaaaa caaaatcaaa tagaaattga ttttgcagaa gaggaaattt ctaaattggt
     6361 tgaaaatgtt gttttaaata catcatatca agaaatgtta tatgatgaaa tagagaaaca
     6421 actggaaatt gattgtatag gtacttggat gatattatct aaattaaaag atggtagtag
     6481 agttcactga ttttaaaata aattattaga gcctaatttt aaggctcttt tttgctgttt
     6541 ttaattaaca atagaaaatt tatctttagt ttgtatataa ttttaatatc atatattcta
     6601 tatatataaa attgaatttg tatgtattat attgtataat ataaaatgaa tttggaaaat
     6661 agttcgctaa aataatttgt ttttataaaa ttactgttta agtttataga ttttgtataa
     6721 aataaagtta tgttattaat ttttttagaa ataaaacttt tactaggaga cagatatgag
     6781 aaaaaatagt attaatatag cactaactgc agcaattaca gcaatctatg cagttttaac
     6841 attatcattg ggatttataa gttatggacc aatacaattt agggtagcag agattatgat
     6901 gcttttggca tttttagata aaggatatat agtaggtctt acattaggtt gttttttagc
     6961 aaatgtaata ggaccatatg gtgtacctga tataatttct ggaacattag ccacatttat
     7021 aagtgcaagt atgatttata ttactagaaa gttaggtaaa caaaataaaa gtactttagt
     7081 tatagcttct ttatggccaa ctgttataaa ttcgattgtg gttggtctaa tgttaaatgt
     7141 attctttggg cttccattaa ttctatctat gatacaggta ggttttggag aatttgtagt
     7201 agtaacaatt gtaggagtac catttttctg ctttttaatg aaaaaatata aaaatgtcat
     7261 taatattaaa ttttaaatag tgtatagtaa tttaattaat tgagagtatt attagtgaaa
     7321 agaggtgttt atttgtcgaa ggtaaaaaga ttgactgatg tttggatata tattaatgat
     7381 aagcaaaagt ttacaataaa agagttgtca gaggaattta atctttcagc taaaacaata
     7441 caacgatatt taaaagaatt aaataaaatg ggacttccaa ttcaagcaga acaaggtaga
     7501 aatggaggtt atagggtgct aaataatagc tatatacctc ctgttatatt tacagaaaaa
     7561 gaagttatga ttatactatt tgcacttaag tcacttcaga tttataatta taagttaatg
     7621 gaaatagaaa ttaattctat aatgagaaaa gtaagttatg aacgtaaaag cagtataaaa
     7681 gtaggtatag aaaatattaa aagatacatt ggctttgtag ctaatgatac taatgatgga
     7741 gattttaaga gtagtaatgt aactgagttt tttagagctt cttcagaatc tctaatttta
     7801 aatattaagt ataagtgtgg taatcagatt ttggaaaaaa acgtatgccc tataggtata
     7861 tatttaaata agggagcatg gttttcacca gcatatgatt gtaaaagtga taaaataata
     7921 ttagttcata tagataatat aattgatata aaaagaacag gagaaagtaa aaaaatacat
     7981 ataaacttgg aagaatggtt acatgaagta tataacaatt tatatggaaa tagttcaact
     8041 actaaaaaat ttggtgaaat tatttatcta aatgtattat taactaaaga aggtgtttct
     8101 aaaataaagg acagttcttg gaatttagat atggagctaa atgaagatgg tagtggaata
     8161 attagtacat tcataaaggt tgaagatata gattgttatg cttcgatttt attttttatt
     8221 ggaaataatg ctaaggttat agagccaaaa tttttgaata agctacttta tgataaggcc
     8281 aatgagttaa gatattttta tgaagaaaat atggtataat atttagaaat gttgaaatta
     8341 aataattatt aaggagatat acgttataat ggaaaatttt aataagaatg ctaaaataat
     8401 tggtattgga gttgaaggta ttaatataat aaatgaagta gaagaaaaaa taaaagcaaa
     8461 tatggatata gaaaaaataa acataaatca agagattgaa aaagaatatg ttagaagtct
     8521 tttagatggt gtagatatat tatttttgat atattcatct gaagataaac atattagaga
     8581 tattataaaa gctgtttcat atatgtcaaa cgaaagaaga gttctatcta taggaatgga
     8641 ttgttcagag aaagaaaata aagaagattt agagcttggg agagagttta aaatcaataa
     8701 tgatagtata tttaaatttg ttgatttaat gaatatcatg gtagagtcta tttctgattc
     8761 ttgcatgata aatatagata ttactgattt aaaagaagct attgtaggag ataaaggaat
     8821 taagtattct tttgaagagt ttgaagatac aaagtcatat agcgagatag ctgacatttt
     8881 atttgacagg atggaatata ttggtgagga atttataagt aagaaaggaa ttgtatttgt
     8941 tgaaggaagt ccagaatttt ctataatgga attgaatgat ttaataagta atattcagag
     9001 caaggtagaa gaaagctatg aagtaatatt ttctttatat ataaaggaaa atttaaatgg
     9061 aaatattaga gtaggcttat tgtataatta gtatatttta gatattaagg agagtgtata
     9121 tgtctgaaaa tataagaggt acagagtatt ggttaatgga agaatttttt tatcaggaaa
     9181 cagacacacc acagattttt atagaagaaa gcataattgc acttggattt gatataaaaa
     9241 taaatttaaa aaaagtattc agtatgggga aaaaatcaga aaagtatatt aatgagaatt
     9301 gtaatgataa agattttata aaaaagttta tgtcgattaa aaaaggagat tattttttat
     9361 ataaagcata tcaaaatata gaagaaaaag ttaaatgtaa tgtgtctatt ggcatagttg
     9421 aagaagattt tgaaaatgga tatgaattaa gtcatatttt agggcataca ctaccagtaa
     9481 aatggatgca taattttgat acagtttcaa attgtaaagt atatacaagt gaattttata
     9541 aattaaataa taatgatatt agttcatatt tagaaattat taagtcacaa gataagaaat
     9601 tattgagaaa aagaatagaa gaaagtgaaa ataataaatg gtatttaaaa aactattatt
     9661 ataaaaatgt agataagtgt gtagatgatg aagaattatt tattcagccc attaatctag
     9721 aacaattaga aagcctaaaa aatataaaga cagatgatta tattttattg tataatagcg
     9781 agaaagtaat ttgtgaaaat gaccatacaa tgaagagtct atttggcgta tgtcaagtta
     9841 tggaagggtt cgataaagag aaagtaagtg ctttggatgg tggatatttt tttcctgtta
     9901 gatggataga gtatggaaag gagaatatag acggaaatgc atgggttata tttgaaaata
     9961 atagtatagt tgaaaagtat gtaaaaagat ttactaataa aatgccatta aatactatac
    10021 tttatggacc accaggaact ggtaaaacat acaatacaaa acagatggtt tacaatctag
    10081 taagtgaaac cagtctaaga aacaaatact atacaggtta cttagattat aaaaacaatg
    10141 tagaattttg tacatttcat caatcatatg gatatgagga atttatagag ggtcttaagt
    10201 ctgatggtga aggcaatttt aagccagaag atgggatatt aaaggatata tctatagaag
    10261 catgttataa tgcattgaaa tttgaaaaaa agatgagctt tgagttggaa aaagatagct
    10321 tgactttaga aactataaaa gacaggaaaa agaaattagt attagaaaat atagaatata
    10381 gtgagagttt taattttgaa gaatcagaaa actatgtttt aataatagat gaaattaata
    10441 gagggaatgt atctaaaata tttggtgagc taataacatt attggaagat gataaaagac
    10501 ttactaaatc aaatcaaact attgtcaaac taccatattc aaaggaaaaa ttttctctac
    10561 ctccaaattt gtatatagtt ggaactatga acacatcaga taaatcaata gcacttttag
    10621 atatagcttt aagaagaagg tttaattttg aagaaattat gccagattat gatttattgt
    10681 ctattgtaga tggtatagat attagaaaaa tgctatatac aatcaataaa aggatagagt
    10741 ttttatatga tagagaccat gttattggac aagcttatct acttgctgta gaaaatatta
    10801 atgatatagt agtagtattt agaagtaaaa taataccact tcttcaagag tatttttatt
    10861 atgattcaga aaaagtaggt ttgattttag gaggaattgg aagaaatgaa aaagataaat
    10921 acattgttta taaagaaaaa cttatagctt ctgagttatt taaaaataat aatgcctcaa
    10981 taatggagag taaagttaac tattgtataa aacaaaacat atctagtgag gagctaaaaa
    11041 atatatatga gtaaaaatat atatgttaag gaaacctatg aatggataag ggtaggtaat
    11101 ggtgaaaatg agcttacaga aattgaatat gaaaaattgc ttaaatatct agagaacaat
    11161 aatgatgtat taaaaagtaa tataatagat attaaataca aaaaaattag atttataaac
    11221 tatgtaggca tcatatgttt tgaaaatgta atacttgaaa tacttcctaa attgtcatta
    11281 agtgataatc ttgtaaaaga tagagagata ctattacaga tgctttctat atgtaataaa
    11341 attccaatta caatgaatga aaaaataaga ttgagtttga aaaattataa tctcttaaat
    11401 ttttttgtta tgtatttcat tgagagtatg caaacacaga tgaaaaaagg tatttatttt
    11461 gaatatataa ataaaataga gaacttaaat gttatgaggg gtaaaatatt attaagtac
//
LOCUS       DAEPLF010000107        11488 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEPLF010000107 DAEPLF010000000
VERSION     DAEPLF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11488
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.107"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(72..497)
                     /locus_tag="KR314_002337"
                     /pseudo
     CDS             complement(72..497)
                     /locus_tag="KR314_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
     gene            complement(970..1257)
                     /locus_tag="KR314_002338"
     CDS             complement(970..1257)
                     /locus_tag="KR314_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328718.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(1277..1552)
                     /locus_tag="KR314_002339"
     CDS             complement(1277..1552)
                     /locus_tag="KR314_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328719.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAIGMLKGVDYEPLIVATQVV
                     AGTNFKFICNATSVTNPPHDYLAEIIVFKPLPCDEHNATITSINIIK"
     gene            1740..2559
                     /locus_tag="KR314_002340"
                     /pseudo
     CDS             1740..2559
                     /locus_tag="KR314_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(2818..5385)
                     /locus_tag="KR314_002341"
     CDS             complement(2818..5385)
                     /locus_tag="KR314_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBH0328720.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTNQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMDGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIALQSEKE
                     LWGLSEWVKQNNSINLYLQNSPAIKIAEDFMSSVMPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     APQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(5388..5993)
                     /locus_tag="KR314_002342"
     CDS             complement(5388..5993)
                     /locus_tag="KR314_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0328721.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(6367..7827)
                     /locus_tag="KR314_002343"
     CDS             complement(6367..7827)
                     /locus_tag="KR314_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBH0328722.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDRAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8158..8736
                     /locus_tag="KR314_002344"
     CDS             8158..8736
                     /locus_tag="KR314_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0328723.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIKRHNLKLKIEKEKAFGVCNS
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            9028..10278
                     /locus_tag="KR314_002345"
     CDS             9028..10278
                     /locus_tag="KR314_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0328724.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            10439..11008
                     /locus_tag="KR314_002346"
     CDS             10439..11008
                     /locus_tag="KR314_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBH0328725.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVDFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAVAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3799 a         1993 c         1587 g         4109 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaattcac gagtgtgcgt
       61 agctattttt taatcaatac aattactgca catatagatg cagaattttt aaatacttca
      121 cctaatgaaa ctgctgcact aatatcatgg gctactcccc tattgtcgct aaaacctgca
      181 gtatcacata attttgcatc cattccttct attggttttc tcatatttac aatgtttggg
      241 tataaagttt gagaaacagc tcctgaacca atctctgcca cagggtctaa gcaatcaata
      301 tatgatatac cagtatcttc atcttctact tccatcatta cacatcctgc aagatagtta
      361 attgcagtac ttttgccaca tcctgtccca ccaagaatta gaattaaatt ttgattttca
      421 gccttattta ttgctacttt tccttcattt aataagagtg ttatttcttc taaaagttga
      481 ttgttatttt ctggcattta ttttccccct tggttatata ttttatttaa ataaagttta
      541 atcccttaag tagattacaa agtttttaga ctatatttaa atttgttttt tatttttaat
      601 aaatttgcat tttcactcaa taattattct tctttgttta ttaaaataac tgtgttgtat
      661 aatataattt ttaccaacac agttatttta tttaatgcgc ttattttatt gtattttatg
      721 taatacagtt gtttttttat attatatgtc cactattttc ctatggttat tacatataga
      781 tttttaatta tatttgtacc attaatttaa acattcctat actactgctt aagttttaca
      841 cctataccag cttgaatatt ttatttttat ttgcttaaat agttcaacta tactggttta
      901 ataggtttcc tttagtttaa atagttcacc tatacctact tgaatagttc acttttattt
      961 gcttaaattc tataccaata ctggcttaaa cattatacta ccatgattgt attgtctatc
     1021 ttcaactaca ataaaaacag gttctaaatc tttagtagat gttattttta attctaaaaa
     1081 aacattatca gttggtaata tttttacatc ataattattt aaattattat tcgtgatact
     1141 attaaataca tcaccttgac gttcaaaaaa aacattatca actattacaa aataaacatc
     1201 ttcttttcta ctattttcaa cctctatagt attttgttta gttacattta gtctcatctt
     1261 ccacctccta ttaatattac ttaattatat taatacttgt aattgttgca ttatgttcat
     1321 cacaaggaag aggtttaaaa acaattatct ctgctaaata atcatgtgga ggatttgtaa
     1381 ctgaagtagc attgcaaata aatttaaaat ttgttcctgc tacaacttga gtagcaacaa
     1441 taagtggttc ataatcaacc ccttttagca ttcctatagc ttcattaaat acttttaaat
     1501 cttcttcttc gacagcccta aattttgacc atccaccaac aaaaagactc atataaaaac
     1561 ctcctttata tatatattta tttatatgat agtaccaatt ttcttaatat tcaatatatt
     1621 acgtctaact ggtcaataaa aatgcataac tggataaaaa gacttataaa atagcttaac
     1681 tttacatata ataagattat tctaaaatat gagacatatc ttgctttgaa tatattctat
     1741 acaacaattt tccaggcctc attgccaagt ttaaggtaac tgactttgga ttccaacttc
     1801 ttgatgctaa taaaaaatat tttgatatgt tcaaagataa tgtaaatttt ctattagatg
     1861 atataaattt agaaggttat cttaacaatg tgtataaata tcataatgat tttaaatctg
     1921 ggagatgcgt ttgtttttct atttctccac aaactattaa tggagatatt tttcacatgc
     1981 aagttgtagc tgaatgtata gattgggaag atgataagcc tatatatctt ttaatatatt
     2041 cagatgtaac gacattggta aaacaatatg tctcattatc atttttcatc caagaatttt
     2101 atcaagaaat atgataaaat agctaaaaaa tatgatattc cttctcacct tatagaaatt
     2161 gagattacag aaacaattgt atttgaagat gtcaaagtat ttagtgaaat aataaatgaa
     2221 attcataaaa atgactttaa atgctctatt gatgattttg gaagtggcta ttcttcaatc
     2281 aatatgctta agggtattta tgttgataca ttaaaattag atagagcatt ttttcaatca
     2341 gaagatttag attctcaacg tgaaagaaat ataattgtat ctataatcga actagctaaa
     2401 aagttaaaaa tgaccactgt agctgagaga atagagacca gttttcaaaa agatttcttg
     2461 caagaaataa actgtgatat gttacaagga tacgtgttct ctagaccttt gcaaataaaa
     2521 gactttgaaa aacttatgtt tcaaaactcc tccaattaac aatataaaat tgcttattat
     2581 aagtgtatta ttgtatcaac aaataaagga attgtctcaa aattgatttt ttctaatcaa
     2641 atttttattt tgagacattc tcattttttt gcataagaaa atacccacca agctacggtt
     2701 tttcttaccg ctgttcggtg ggtattagta gtattatgaa atctgaaaac tttaagtatt
     2761 actacttccg ctaacgcaat cacaaaggtc gaaagcttca aagcgaaaat aggcttttta
     2821 ctcatcaaac gtcaccgtac ctgccaaccc gtccaccgtt atcatttgtc cgctcttaat
     2881 agttcgtgtg gcaatgtgtg tagccataac ggctgggata ccatattcac gtgcgacaat
     2941 gcttgaatgg ctaagaggac caccaatgtc cgttacaatc gcacttgcag atgaaaataa
     3001 tggtgtccat gctggagttg tggtaactgc aaccaaaaca cttccaggtt gaaagctttc
     3061 aaaatctgct ggactattca gtacacaagc tggagccgtc acaacaccag tactggtacc
     3121 aatgcccgtc aacacaattt tcccatcttt ctgcttctgt ggtgcttgtg atatagtttt
     3181 tttgtttttc tctggcaact tggacggtga cacataaccc atgtattttt taagttcagc
     3241 ttttcgaaca ggtattatcc cgttcatatc agatagagac ttgtttttat ccaactgctc
     3301 tataagtacc tccaattccg attttgtgag ccaataaata tcatctaaat gtgaaatagc
     3361 tcctcctcgt atgaatcgtt ctgagatttc ctgaaacata caacgaatca gaggatgtcc
     3421 cattcccatc aaataaatag cattttcacg cataggagag gtttcctgtg cccaatggag
     3481 caacttaaaa aatagttttt tacgcaatcc acctatgtgt tgtaaaatct cttcttctgc
     3541 atgttttcta cgcttttcaa acgtggtctg gcgtaaaaaa ggactttccc cttttccttc
     3601 cacaaatgtt tttacggatt caaaagttgg tgtaagtgtt tcctgcggtg tggaatacgc
     3661 aaaatcaaac tcataagcgg tacgaccaaa ctctttaaag tacttattga ttcgattttt
     3721 ccattctatc catacttcct gtgaaactcc aacaggcata actgaagaca tgaaatcttc
     3781 tgctatcttt attgcaggac tgttttgcag ataaaggttg atgctgttat tttgctttac
     3841 ccattctgaa agaccccaca actccttttc cgattgcaga gcaatcgtat caaacccaag
     3901 caggaaaacg gaagtatccg ttataccagc acgtcgaact gctccttgaa ataattttgt
     3961 aaataatgtt tcactgatag aagcagcagg caatgtaagc tgaatccttg tgaaataaat
     4021 acatgcggca taaaatattg tctgaatacc atccataatt tgatgggcgt tcatcgtatg
     4081 caggggcttt tcttcccatt gtttaatagc atcctcatat ccttttcgtg ccgtttccca
     4141 gcgtgcaacg ctgttcataa gtgtcctacg taaagagcgt ggggaaaggg atttgatagc
     4201 aatcaaaagt ggctttgatt tagcagaaag ataaacatat ccattaataa ccgtatatgc
     4261 gccgtttttt ggtaataatt ttttcgcact tttaccaaac atatctatcc ataaaagctc
     4321 cgatgcacgg tttacgattt caagaccaag cgtggcgaac aaaggtgtaa caggatttgg
     4381 caagtgttct gccaaacttc cctttgtgta tacaacatcc tgttctggca atacccattc
     4441 cggtggcaga acagtaattg gacgagcttg aacaatgtac aatttatctt tttccagtgc
     4501 ccactctaca tccataggca tttgataata cttctcaatt ttctttccaa gctgtatcag
     4561 ttgcattacc tgattacgag taagagcata tttttttctc aacctttcag gaaccatggt
     4621 ttcttctgtt ccttctgaag tacgaaccgt cataatctct ttatttgcca cttcatacga
     4681 tataattcgt tcggagtcct tatccactac gattgtatcg ggtgtaacta agctagaaac
     4741 caccgcttca ccaagccccc atgcagcgtt aataatcatt tcgctacgtc taccattgat
     4801 tgggtttaac gtaaacataa taccagatga atcggaaaat gcaagctttt gtactacaac
     4861 agcaagtgcg acaatctcgt gctttatatt attcttcaca cgataagcaa tagcacgagc
     4921 tgtccataag gaagcccagc atctctttac agcgtcaata actttatctt caccttgtat
     4981 gttaagataa gtttcctgct gacctgcaaa agaagcgtca ggtaaatctt cagcagtcgc
     5041 agacgaacga acagccaccg atatgttccc taatccggca taagctgttt taattgcgtc
     5101 cgacacttcc tgtggcattt ctccattatg gaaaagctcc cctattttct tagatacatc
     5161 ttcaagttgg ttggtattat tggaatcaat accatctagc agtttattaa ttcgagactg
     5221 aatatggttc gtctcaacaa aaatctggta tgaagcagtc gtaacatgaa aaccgtctgg
     5281 cacagggatg cctgctgtca atagttttga taaagacatc ccttttccac caacaatttc
     5341 aatggttgct tgtttatgtt ccagtggcaa gacataataa atcataatca ttcacctcca
     5401 tcattcataa ttccgtgcag aaatatattc attgtttcat ccaacatttt ttccgaacta
     5461 tccgatggtc gtgacgtgta gctgattgat aacatggagt taattaacaa acgtgtcaca
     5521 agagcggtat ccgttttgcg aaagatgcct gtggtcacac cattatcaat taccgatttt
     5581 ataatatctt gatatgcata acgagcatct ttaagtctct ttcgatactc ttcttctaaa
     5641 taatcagatt ctgtatttaa ccacatcagt acagttctat actgctctgg aaatctaaga
     5701 tgattaagca tgatttttct aaggcactgt tgtggtgaag gttcaccatc aataatccta
     5761 tgaacttgtt ggatagtttt ttctatttct ttctcaactg catatacaac aatctcgtct
     5821 ttcgttttga aaaaatcgta tagacttgac tttcccatgt tggctaaaac tgcaatttct
     5881 ctcattgatg ttttttggaa tccattttca gccatgagac tcaaagcaac agcaactatt
     5941 ttttctcggc gttctgcttg ttgttcaggt gtaagcacaa ttccttttgg catagggcga
     6001 tacctccttt tagcgaccaa tggtcgtttc ttttttattt atcatacagc gaccatcggt
     6061 cacttgtcaa ggttaatttt tttcattgta tatggtactc gagataaaaa tgttcatttt
     6121 agaaattgct ctatttaagt agaaataggc ttcatcatta gaaaacaaga ctataaattt
     6181 gagatattta tcatgttcaa atttcatcaa tatcaaagtt taagatatta ataaaaaaag
     6241 aattactaag aaataacata taaagattca aaaaaggagt tgaacttaaa attcaattac
     6301 aaaaaatcaa atttaagaca actcctttat tctttatctc tactattctt tatctctatt
     6361 tatttattac aactccctaa catttaccac tactgcctta ggtctagtca tagcctcgat
     6421 tgaatactta acaccttgag ttcccatacc agatgccttt acacctaaaa atgggaagtg
     6481 gtctggacct ctttcagtct tattatttat ttgaacagta ccaacttcaa gtttatcagc
     6541 tatataaaat gccctatcaa tatcacttgt aaatactgat gattgaagcc catactctga
     6601 ttgattagca atttcaattg cttgatttat atctttaact cttataattg gaagtactgg
     6661 accaaatggt tcttcccaag caattttcat atcaacattt acattatcta acaatgttgg
     6721 atagaataaa ttattttttc taacattacc tgttattaac tttgctcctt tatgtaatgc
     6781 atcatcaacc aacccttgaa caaaatcagt tgctttatta tctataagag gagtgataac
     6841 tacttcatcc attggatttc caattgtcaa cttctctacc aatggcttta tttttccgac
     6901 taatttatcc gccacactct cttgtactaa aattctcttt actgccgtac atctttgtcc
     6961 tgaatatgaa tacgctccag atactatatt cttagctgca aaatctaagt ctgcatcttc
     7021 taatactata gccgcatctt ttccaccaag ctccaataat agtggtacca ttccagatat
     7081 tttagatata tgctgaccaa cttcagtact accagtaaag ttaataaaat taattccttt
     7141 atgcgttaca acataatccc ctatctcact acccctacct gtaatagtat taagtactcc
     7201 atctggtatc ccagcttttc tcatgatttc taccacatga agtgcgctaa tagccccttg
     7261 tgtagcaggt tttaatacaa ctgcatttcc accaataagc gctggagcaa tctttgacat
     7321 tgataaattt acaggataat taaaaggaga tatagctaaa acagtaccta atggaaccct
     7381 tgaaacataa gacatcttat ttcttgttcc accagggaaa ttatctccac taatggcttc
     7441 accctccata ttttttccca catcagctgt gtatcttaaa aaatctgctg ttctctcaac
     7501 ttctgccctt gcagatttta tgtctttggc tatttcaagt accagtatat tagctatctc
     7561 atctatattt tctaacaata aatcagcagc cttatgaaat atattggctc tcttgtatac
     7621 aggcatttca gcccatactt ttatactctc ttttgtgttt tttataacct catctacctc
     7681 atgttttgtc atagattgta ctttcccaac aagagaatta tctattggtg agtaaatttc
     7741 tattggcttt ctcgaattag attctaccca ttttccattt attaaatttt tatatacatt
     7801 ttcttttgtt ttaagttcat taaacatatt tttattcctc tctctgatat aactttgtgt
     7861 atttaaatta tagccattta ttcgttatgt aaacatttct ttttttcata aacgtttacc
     7921 tataaaaaaa attttaagtt aacgtttttc tagattcttc tattttaata acatactcgc
     7981 tccaaatcta tataggtcac actttaatat caatatacca atataccaat ataccaatat
     8041 aaatattata aacataaatc acttgacaaa tttataagta gacaatatta tatatctatg
     8101 acttaaaata tttttaaagt catagatagc atatgcagat agaaaggatt gataaaaatg
     8161 gctaaaagtt ttacagaaat agaaaaagac aatataagaa aaagactaat ttcagaatgt
     8221 gaaatcaact ggtctaaata tggatacaaa aaaactaaca ttgatgaact ttgttcaaaa
     8281 tcaggaatcg caaagggttc tttctataca ttttttgatt caaaagaaaa gttatttttt
     8341 gaagtactaa atggaatcca aaacagactt atatcatcag tagatgaaat tttaagtaac
     8401 atgcctccaa aagaaggctt tgcaaaatcc ttaaaaatgt tatatcgatt atatgataaa
     8461 aatccatttt tatattcacc aaataatcct gatttcatca gcctaataaa taaactacct
     8521 caagatatgt ttttacaact tgagtccaat aacatacaaa gtttttacga cttaattaag
     8581 agacacaatc tcaagttgaa gatagaaaaa gaaaaagcct ttggtgtttg taattctcta
     8641 ctatcaagtc tattggtaaa agaaaatatt gaatataact atattgaagt atttgatttt
     8701 atggtagata atcttgtaga acacatactt gaataacaat ttaaaataat ttttctaaat
     8761 atttctttat actaaagagg attaatatga ttgaattaat gaaaaaaata aacaatatca
     8821 tttacaaaat aaatgataaa ctaaatatac acacaaaact tatagacccc agagttaact
     8881 cattccactc tactctaagc catctataat agttcgtata tttctaataa tattagatat
     8941 taaagtaaga aaaaaattcc ttataataat tgctattata tcaattatta taaatatttt
     9001 aataatctta gataggtagg tatatatatg gtaaaatcct caaacacttt taataaaaac
     9061 ttcaacttga tgatattagg tcaaataatc tcattgtttg gtgcatcaat acttaaattt
     9121 gctttatctt tatacatact tgatataact ggtaaagcag aaatatttgc tactatacta
     9181 gcagtatctt caatcccaat tatagtattc tcccctattg gtggagctat tgcggatata
     9241 ttcaacagaa gaaacttaat ggttattttt gatttttcaa gcagtcttac agtactaatc
     9301 ttagcagtat tcttattcaa caataatggt tcaatattac ttgtaggaat tattatgacc
     9361 atattatcag ttataagcac aatgtatcaa ccaactgttc agtcaagtac acccctttta
     9421 gttgataatg agcatttaat gaatgcaaat ggtattgtag ctggagttgc atctcttaca
     9481 aatattgcag gacctgtatt aggaggtgtc ttgtatggtg tgattggaat taatgcaatt
     9541 atagttataa gttgtgtaag tttctttcta tctgcaatta tggagatatt tatacagatt
     9601 ccttttatca aacaagagca aaacagcaac attataaaaa ctatatttac tgatataaag
     9661 gatggtattg ttcatatatg taaagaaaat agatttatac taaaaatact ttttttagct
     9721 atagcaataa acttattttt aagctctatg ttaatagttg gtatccccta tatagtaaaa
     9781 atcactatgg gagctagtaa cttcttatat ggtctagcag aaggagcaat ttcttttagt
     9841 tcactggtag ctgctgttag tattggtatt ttctcaaaac atctaaaaat cactaatttg
     9901 cacatttcct ttattatatg tgcagttatg ttcataccaa tggcggtttc agtatatcca
     9961 tttatattaa acacaggatt tccagttcca tttttaatct ttatagtatc atctatgcta
    10021 atatttttta caacgactct tatctcaata tatgttataa cagtcacaca attaaaaact
    10081 ccaaatgaac tcttaggaaa agtaatgtca attctttttg caggaacagc aattgcaatg
    10141 cctcttggtc aggttatgta tggtaaactt tttgatttat tatcagatag agtgtacatc
    10201 attatactag gagttggatt tattacacta cttatagcat ttgtatcaaa gataacattg
    10261 aggaacgaaa atctttagat acaaagaatt tagatataaa gatttaattt tcactaattt
    10321 ctgaatttaa atgcaaaatt gcgtatcagt atgttgacgt tttagaaatt gattgatata
    10381 atcaatggaa aataaacttt ataaaaattc aaaatattca gctagaggag tgataaaaat
    10441 gagttacatg ccaactttag aacaagcatg ggaaatatta agaaaataca ataaggaaga
    10501 atttcatata agacatgctc aaattgtatc tggagttatg agatactatg caaaggagta
    10561 cgaccctgag agagtagact tttgggaaat agttggactt ttacatgatt tagattttga
    10621 aatgtatcct gatgaacatt gtgttaaaca aagagaatta atgacagagc ttgatttaga
    10681 taaatcaata atcgattcta caataagtca tggatatgga ttaactggtt cagatgttaa
    10741 accagagcaa tttatggaga aagtattatt tgctgtggat gaacttactg gtttaattgg
    10801 tgctgttgca ataatgcgtc cttcaaagag cgtttctgat ttagaattaa aatctgttaa
    10861 gaagaaattt aaagataaat catttgctgc tggctgttct cgagacgtta taagaagtgg
    10921 tgcagaaatg cttggatggg aacttgataa gcttatccaa acaactatag aagctatgag
    10981 aagcttgatt ccagagatgg caatatagct cctcatacat ataccataga agttacatct
    11041 ttaaagagca tttctctttt tgatgcaact tctttttata ttttgtagaa taaaaagttt
    11101 aatattttaa actcactata taatctataa taaatactat aaataatata atgtataata
    11161 gtcataaact ttctaacctt aaaagtttct aatactcaat aaacaattct aatatacaag
    11221 tggcattata tgatttaatt atatatcttc aatttatata ttaaaataaa tatatatatt
    11281 aaattcttat acaccaaatt tttacattaa acttctatgc accacattta tacatattaa
    11341 attcttatat attaaatttc tatgcatcac ttttatacat attaaattcc tatatattaa
    11401 acttttatgc gtcactttta tacatattaa attcctatat attaaacttt tatgcatcac
    11461 ttttatacat attaaattct tatatatt
//
LOCUS       DAEPLF010000108        11476 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEPLF010000108 DAEPLF010000000
VERSION     DAEPLF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.108"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            104..541
                     /gene="spoVAC"
                     /locus_tag="KR314_002347"
     CDS             104..541
                     /gene="spoVAC"
                     /locus_tag="KR314_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBH0328726.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            695..1708
                     /gene="spoVAD"
                     /locus_tag="KR314_002348"
     CDS             695..1708
                     /gene="spoVAD"
                     /locus_tag="KR314_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBH0328727.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            1722..2084
                     /gene="spoVAE"
                     /locus_tag="KR314_002349"
     CDS             1722..2084
                     /gene="spoVAE"
                     /locus_tag="KR314_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBH0328728.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            2267..3283
                     /locus_tag="KR314_002350"
                     /pseudo
     CDS             2267..3283
                     /locus_tag="KR314_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
     gene            3658..4500
                     /locus_tag="KR314_002351"
     CDS             3658..4500
                     /locus_tag="KR314_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBH0328729.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            4515..4979
                     /locus_tag="KR314_002352"
     CDS             4515..4979
                     /locus_tag="KR314_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328730.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIITCFGQMIALKTQAQDLLIGSISG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            4966..6138
                     /locus_tag="KR314_002353"
     CDS             4966..6138
                     /locus_tag="KR314_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0328731.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            6182..7117
                     /locus_tag="KR314_002354"
     CDS             6182..7117
                     /locus_tag="KR314_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBH0328732.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(7916..8314)
                     /locus_tag="KR314_002355"
     CDS             complement(7916..8314)
                     /locus_tag="KR314_002355"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBH0328733.1"
                     /translation="MKWKELYNNARGKLNPRTISPFIDAGGVAAAILTDSGNVYTGVC
                     IDTACTLGMCAERNAIANMITNGESKITKLVCVMSNGSVGSPCGACREYLMQLDKDSA
                     CIEILVNVETEKTVKLSELLPDWWGSDRFI"
     gene            complement(8613..11306)
                     /locus_tag="KR314_002356"
     CDS             complement(8613..11306)
                     /locus_tag="KR314_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBH0328734.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQST
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTTEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANQG
                     VYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
BASE COUNT         3986 a         1644 c         2037 g         3809 t
ORIGIN      
        1 ttaggcatct tttttatttt ttgaccaaac tttagatagt caaaataaat aataggttta
       61 tttttttaaa attaacaaat aatactttag agaggtgttt agtatggata aaaattataa
      121 aaaatatgta gaccaaataa gcccaaaacc tacatattta aaaaactata cacttgcatt
      181 tattgtaggt ggaattattt gtatgatagg gcaagcaata aatgatttgt atatgaaagt
      241 tggaggtctt gataaactag gtgctagttc tgcaacatca ataacattaa tatttatagg
      301 ggcattttta actggattag gagtttacga cctaatagga aaaagagctg gagctggttc
      361 tataattcca ataacaggtt ttgctaactc aatagtatct ccagcaatgg agtataaaag
      421 agaaggctat gtattaggtg taggtgcaaa cttatttaaa attgcaggac cagttttagt
      481 ttatggtata ggttcttcta ttttatgtgg aattatttat tatattttta agatgtttta
      541 gatgattttt atttaattta aattttatta attatgaaga aggtataaaa tctgaaaata
      601 gctactaaag taactaattg aaaaataatt gtgcagattt aattaatatt aaaaatttga
      661 atagtaaaat gcaaaattaa aaaggtgtga aaatatgaaa aataaaagaa ttggaaaaag
      721 aacagtcaag ttagaaaata aaccaaccat aatatcaact ggaactatag tgggtcctaa
      781 ggaaggtgaa ggaccactaa aggactattt tgatatgatt atgactgacg atttatatgg
      841 tgaaaagact tgggaattag ctgaaagtaa aatggtagag actgcatccc aacaagctat
      901 ccaaaaggca ggtaaaaaat tgtcagatgt aaattatatg ctaggtggcg atttaataaa
      961 tcaaatagtg ccagcatcat ttgcagcaag agaactggct attccatttt taggtatata
     1021 tggagcttgt tctacaatgg cagaaggtct ttgtataggt tctatgctta tagatggtgg
     1081 ttttgctgat ttagtactgt caggtacatc aagtcactat tgtacagcag aaagacaatt
     1141 tagattccca cttgaacttg gaaatcaaaa accaatgaca gctcagtgga cagttacagg
     1201 agcaggaagt gtattattag ctcctaatgg agacggtcca aaagtaaagt atgttactgt
     1261 tggtaaagta atagatgagg gtattgatga tggtaataat atgggaccag ctatggctcc
     1321 agcagcaata gatactatct actcttactt tgaagatact aatgatgacc caaatagctt
     1381 tgatataatt gctactggag atttaggtac tcttggaaaa cagatagcag aagactttct
     1441 aaaagaaaaa ggtgtagata tatcaaaagt ttatactgac tgtggtatag agatgtttaa
     1501 ccttaaagaa caagatgtcc actgtggtgg aagtggatgt gggtgttcag ctacagtgtt
     1561 tgcaggatat atatatgata agttgagaaa aaaagaattt aacaaagtaa tgcttgtatc
     1621 aacaggagca ctactttcac cgacaagtac actacaaaag cagacaatac catgtgttgc
     1681 acatgcagta gtaatagtaa atgagtagga agtgatataa tatgttaatg gactatgtaa
     1741 gagtatttat tgtaggtgga attatatgtt taatagccca agtaatgatg gattatttca
     1801 aaatgcaaac tccatatgtt atggtaacgt atgtaacaac tggtgtagtt ttaacttttc
     1861 taggacttta tgagccatta gttaagtttg cagcagcagg tgcaacagtt cctataatag
     1921 gatttggata ttcacttgct acaggagtaa taaaatcaat tcaaagtgat ggttttttag
     1981 gtattttttt aggaggtaca actgctacag ctggtggaat agcagcagct atattctttg
     2041 gatatattat gtcagtagta tttactccaa aagcaaaacc ataaatcttt attaaaagaa
     2101 taataagttt ttgaaataat aactttttat aaagcaggtg gatatctttt agattccacc
     2161 tattttttat ataaagaagc gtttacataa ttgaagttga caattattta caacatagtg
     2221 tagtattata ttatcaaaat aaaatgaagt gaagtagggg ataaagatgg taactattaa
     2281 agatattgca aggaatctag gtatcagtta ttcaactgtt tctaggtgct taaacaataa
     2341 tcctaatgta tctgaaaaaa caaaaaagaa agtagtagaa gaggcaaata gactagggtt
     2401 ccattttaat gtaaatgcaa gaaatttagc aaaaaaagaa actaacagga taggggtaat
     2461 cttttcaaat aattttaatc atcaagatac tagaaagttt tttagtgata taatggatag
     2521 ttctataaat tctattgaga ctaataaata cgattttata atacagccta ataataacat
     2581 tagtggtgat agtaatgtat ataaaatggt aaatggacaa atggttgatg gattagtaat
     2641 tgtgtctcaa tcaataaaaa aagaagagta tgatttttta aaagacaata attttccaca
     2701 tgtatttata tattttaaac catcttttgt aggagaagta gacaatttct tttgggacga
     2761 taacgtgtat ggaggttata tagctaccaa gcatttaatt gaccatggtc ataaagacat
     2821 aataacagta acttcagatg ataggagttc aaaaatgcat gaagatagaa ctaagggata
     2881 ttgaaatgct atggaagagg caaatttaaa gaccgaagtc attaaatcta aaatggattt
     2941 tgaatctcaa gttgaatttt tagaaaaata tattgataaa ataaaaaaag cttcagctat
     3001 atttgtacaa caagatgtac ctgcgatttc tataatacaa gagttaaaaa ctacttatgg
     3061 tataaatgta ccagaagata tttccataat agggtataat aatattgaat taatatcata
     3121 ttttaggtcg catttatcaa caatagatga cccaagagag caagttataa aaaatggagt
     3181 agatagttta gtaaatataa taaataaaaa gagtacagaa cacattccaa gaaaattata
     3241 tccaaggtta ataatacgaa atagcgttaa aagaataaag tagtaagctt ttaagtatca
     3301 aaagtttatt aaaaatatta tagattatgt gaagtttttt aacaaacaat ataaataaaa
     3361 attaaaagga gtaggtgtat ataagtacat tgtactcctt tatttattga gatagttttt
     3421 ataatatgaa tgaataattt tattcaatta tttgaaaatc cgttatttta acaatataaa
     3481 ttaataaatt atttgtattt tatatcatga atctataata tttttttgat tcataactaa
     3541 aagtatataa aaatgatgaa taaatatata ttatacatta tcaatgatag gttatataat
     3601 aaaatcacta acaattatat tataagaaaa ttataaaaat tacaaggagg tatagaaatg
     3661 aaagaaaaag cagggattag aaatgccata atagcagcct ttataatcac aatattggca
     3721 gaattaattg gacctatttc atttaaagtt atgggaataa atgtaacttt acttagtata
     3781 ctatgggcta tattcatagg aatggctgtt tctcctcatt tattagggag agttatacca
     3841 gccttaaaaa agtttatagg ggataatgaa ataaatgtat ctccatattt attatctttg
     3901 acactatatc cattaggaat aatgtttgga attaatgcag ggcctaaagt tggtatatta
     3961 cttcaagcag gaccagcact tttattacaa gaatttggtc atatgggtac aatgttgata
     4021 gcattacctg ttggagtagc attaggtctt ggaagaagtg cattaggtgg tactttttca
     4081 ttatgtcgtg acacagcact aggcattatt ggagataagt atggattaaa ctcaccagaa
     4141 ggtataggaa cactaggaac ttacataagt ggtagtatat ttggaacatt attattttca
     4201 tttttagcac cagtgggtgt acttttagga tttcatccat atgctttagc aatggcatca
     4261 ggaatgggta gtggttcaat gatgggagct gcaacagcat cattaatgaa tacggttcca
     4321 caaatgtcag agcagatttt agcatattca gctactagtg gattgattac agctgttact
     4381 ggaatatata tagaattatt cttatcttta ccagtagcca actactatta tagcaaagta
     4441 gctccagtta ttgaaagatt tagaggaaga aaaacaagaa gaacaagtaa taaattgtaa
     4501 aggaggatat atatatggaa aaaactttta agactattat ggagcagata aaaatagtat
     4561 tgtttatata tattattact tgctttggac aaatgatagc acttaagact caggctcaag
     4621 atttgttgat tggaagtata tctggatttt tgttggtgat aattgctatt ataataaaga
     4681 attttgttaa aaaacctaat ttaccaggtt ttgcatgggc tacattagtt gcattcgttt
     4741 tatcgcttcc aatatctcca gtgggggata tcatagcaac aaatgtaggg aaaatcaatt
     4801 ttatgtctac agtaacacca ttattagctt ttgcaggaat ctctgtaggt aaccaattag
     4861 atatattaaa gactttgagt tggaagttag tagttatatc atttatagtt atgacatcta
     4921 cttactttgg ttctgctttt atatctcaaa cagttttaag attaaatgga atgctttaag
     4981 gaggtagtaa atatgaatga attaaaagca aaaattaaag aggaaataaa gttattatct
     5041 gaagagaaaa agacatcatt tgaaaaagtt tctgactatt tattttcaaa tccagaatta
     5101 gcatttgagg agtataagtc acaaaaagca ttatgtgatt tgttagaaga aaatgaattt
     5161 aatgtaacta aaggtgtagg aggattagaa acatcttttg aagctgtgta ttcaaatgga
     5221 actaatggga aaacagttgc ttttctagct gaatatgatg cacttccagg aatgggacat
     5281 gcatgtgggc acaatatcat agggacaagt tctgttggtg caggtatcat attaaaagaa
     5341 atcatgaaaa aacataatat agaagggact gtaaaggttt ttggaacacc tgcagaagaa
     5401 agagtaggtg gaaaaataac tatgattaaa gaaggtgtat ttaataatgt agatgctgca
     5461 ttaatcttac atccaagtga tgcttcaatg cctgatgata tatcatttgc tcaagttaat
     5521 ttaaaatttg attttactgg taaggcatca catgctgctg cttttccttg ggaagggaaa
     5581 agtgccttaa gtggagtaat agcactattt aacagtgtaa atagcatgag attacatctt
     5641 aaagattatg caagagtaca tggaattata actgatggag gtagcataca taatataatt
     5701 cctgaaaaat ctacagctat atttaatgta agagccttaa gcatagaata tttaaatgag
     5761 atttgtgaaa tgcttaaaaa ttgtgctaaa ggagctgcaa tttctactgg aactaatgta
     5821 gaaatagttc aattagatga aatatacaaa gagattaaga atgattctga attagtgaat
     5881 atagttagac aaaattttga agttttgggt gaagactatg ttgaaagaga tttaagtcaa
     5941 ggtataggtt caactgatac aggaaattta actcatgaga tacctgcaat acaagcatat
     6001 ataaaattaa aagaaaatac tgcaactcat acagatgaat ttgctgttgc agctggtggt
     6061 gaagaaggca aagtagcact tataaaagca attaaggtgc tagcaatgtg tggagtagat
     6121 attttgtata gtaaataata tataatttta aaatatatta agaaaggaaa tcggattaga
     6181 catgatatta aaacaagtaa tagaggttta tgacatttta gataaagcaa atgctaatgg
     6241 ggaagaagta aaaacttact tacaaggtta tggtgaagta gatgtaactg taaaagaatt
     6301 gtcttcaagt aaaggttcaa cagatttagt aaagcttaca attcctggga aaaatggaaa
     6361 attaaaaggt ggagatgctc ctactcttgg tattttaggt agattaggag ggattggagc
     6421 aagaccagaa gttataggtt ttgtctcaga tggtgatgga gcattggtag caattgcagt
     6481 ggctgctaaa cttttggata tgcaacgaaa aggggacata ttagatggtg atgttgtaat
     6541 ttcaactcat atttgcccag atgctccaac taaagaacac tatcctactc cttttatgga
     6601 ttctccaatt gatatgatga ctatgaatga aaatgaagtt gatagtagtt gtgatgcaat
     6661 actttctata gatactacta aaggaaatag agtaatcaat actagaggat ttgcaatatc
     6721 gccaactgtt aaagaaggct acatattaaa aactagtgat aaccttttag atattatgca
     6781 gactgtaaca gggaaatctc catttgtatt ccctttgtct attcaagata taacaccata
     6841 tggaaacaat ctgtatcact taaatagtat tttacaacca gcagtagcta cagatgcacc
     6901 aatagtagga gtagcaatta caacagagat gcctgttgct ggatgtgcta caggtgcaac
     6961 taattttagt gatttagatt cagctgggag atttgcaatt gaagttgcca agctatttgg
     7021 tagaaataag tgtgatttct atgataaaga agagtgggaa atgcttatta agagatatgg
     7081 caaattaaat agatttcaaa catttggtat acagtaatta gaaaaatatt aagtttatat
     7141 aatttgtgat acaactattt aagaagatat aaacaattag gaataaaagt aagaataaag
     7201 cacttagtta aaaactttaa aaaaaaatat ttatttgttt atataaaaag gaggattctg
     7261 gttattggga ttctcttttt tatgaataca aataatatat tgtaattata aaaaaaggag
     7321 cgtttcaaag tgattttttt aatcaccttg agacaactcc ttatattttt tatttaaatt
     7381 ctatttttta ttgactgtat agctattttc accatcataa ggtggcatga ccttgaacag
     7441 cgtggttttg taaagttggg caccacaaaa caaaaaatca acaagccctt gtgtggtctg
     7501 ctgacgtgtt tactatacta tactttccca aaaaccgcaa gcactatggg aggctataag
     7561 gcggcgacca ttcaggggcg atcccctcga aaccgggata gggttcccat gtgggacata
     7621 ataaagagcg ccccggaggt atcaactacc atccggggcg ctctttatta tgtcccgtgg
     7681 gaaccatgcc cacgaagcag ggcgtacaac ggacagatag gtcctaccct cctgacagac
     7741 tggaatctca atcccatcga aaacaatcat aacaatcatg gcaagattat atacactcct
     7801 gtaaaatatt tttgtgtgta ccatgaagta ctgcttgcca ctattgagaa cggataaaag
     7861 ggaagtacgt attataccaa taaggtgaac gaaacaaaat actcatttga taaagttaaa
     7921 taaacctgtc ggaaccccac caatccggca agagctcact caatttaaca gttttttctg
     7981 tttcgacatt aactaaaatc tcaatacaag cactatcctt atcaagttgc attaaatatt
     8041 cacgacaagc accacatggt gagccaacac tcccattgct catcacacaa actaatttag
     8101 taattttact ttcgccattt gttatcatat tggcgatagc atttctttct gcacacattc
     8161 ccaatgtaca ggctgtatca atacacacgc cagtataaac atttccgctg tcggtaagga
     8221 ttgctgcggc aaccccacca gcatcgataa aaggtgaaat tgtgcgtgga tttaattttc
     8281 ccctagcatt attataaagt tccttccatt tcatcaggtt gatcctcctg ttgtatccat
     8341 ataaataatc ttttatacgc cttttccgcc taaggcacac aacacattac tcgctttttt
     8401 cattctaata ttcgtgcgtg aaaatgtcaa acggtttact tcgtttcatt acttcatact
     8461 gctgttcata accctgtttg ttcacttggg caccattcga gcaccaattt acccgtttca
     8521 gggttgtagc gaacacagca gaaatgctca tttcatgggc ttttctggct agaaaaaaaa
     8581 ttttatcgct gccagatatt tactattttt tattaagttg ctggagttcc accaccatca
     8641 ggaggagtag tagagccacc accattattt ccaccattat caggaggagt agtagagcca
     8701 ccaccattgt ttccgccatt atcaggagga gtagtagagc caccaccatt gtttccgcca
     8761 ttatcaggag gagtagtaga gccaccacca ttgtttccgc cattatcagg aggagtagaa
     8821 ccgccaccat tatcacctgt gttattattg ttattattat tttcttctgg ttggttattg
     8881 tctggttttt ttgtatcttc agatgatggt tgagaactta ctttagtagg agcagtacct
     8941 tcaataaatg ctgctttaga gccagaagat tgctttcctc catcctctaa attaattttt
     9001 gtaaaatata catttttagg aacttcaaat tcagtaaccg ttaaattagc atgtattttt
     9061 tccataattt tttcccatag ttttgcagta ctagtacttc catgtggtac ttctctacgt
     9121 tttatagtgt taccagaatc atcttttcta ccagcatcat ctccaatata agttgcacca
     9181 acataataag gagtgtaacc gacaaaccaa gcatcatatg cactatttgt agtacctgtt
     9241 ttacctgcta caggcattcc ctttggaagt gtagcaacac caccagtacc ttcagtaatt
     9301 acgctttcta acatatctgt aacaacataa gctacttcag ggtctacaac tttatgttct
     9361 tcaggagcat ttttaactaa taattgacca tcaaaggtag ttatagtagt aaaaatagaa
     9421 ggttctacat aaacaccttg atttgccaat gtaccataag cagcagccat ttgaagtgga
     9481 gtaataccat tcgccatacc acctagtgtc aatgatggga attttctatc acttgcttca
     9541 gatgaacctg agtgattatt ctttaaagtt gttataccaa agttctttaa gtaatccatc
     9601 attatatcta cacattcatc ataagagtct ccaagcattt cagtagtttt gacagctatc
     9661 gtgtttgaag atttagcaag tgctttacgt aaactcatag aaccatgacc agcagtagta
     9721 gttcttggat tccatttatt attttcttca aacttatatc ctcctcgaga atcacttaga
     9781 gcagttgatt gtgttatttt tagggtatca atagcaggtg tatatattgc taaaggtttt
     9841 atagaagaac ctggctgatg agggttagta gcacgattca aagtttttct accacttata
     9901 tttcttcctc cagctagtgc tcttatttca ccatttttat aatctaagat taccattgca
     9961 gcttgtggtt ggctaactcc atcagggccg accatatgtc ctgggaaatt gctatttcta
    10021 tcaaattctt cttctaaagc atcttgcatt ttagggtcta tagttgtgtt tacaattaga
    10081 ccaccattgt agaataagtt ttgagcttca tcatttgtat gtccttgttc cattaaagca
    10141 tttataactt cactttcgat aaggtcttct acagatgaag aaactttatc tgatgattga
    10201 ggcaatttta tttctatttt agctttgatt gcttcatcat attcactttg ttttatatat
    10261 ttcaattcta acattttctt taaaacaatt tcttgtcttt ttttagcttc agggttactt
    10321 acagcctttc taacaactaa tgtacctgct tttaattgtc tataagtatc attagatata
    10381 agctcccaag attttatttt ttcaatcata tcaaagtcca cttgagtagg gtcatctaaa
    10441 tcatcatcag ttgtgttaac aaaaaataat aatttatttt ctaaatcctc cttagtttca
    10501 tttccctcta acttagaagt tttataggct gaaaatcttg atggattttt agttgaacca
    10561 gcaagaagtg ctgactctgc tagagtgagg tcagctgcag acttatcaaa ataaccacgt
    10621 gcaccagcct cagcacctgc tagacctcta ccaacgaaga agttatttaa ataagtttct
    10681 agtattttat ctttacttac tgttttactc atctcatgtg catagtaaat atcctttatt
    10741 ttacgaggta tagtttgctg ttcagttgtt aacagcattt tggatacctg cataggaata
    10801 gtactaccac cttgcttagt acctgtaagg gtctttaata ctgacctaaa taaaccttta
    10861 atatcgacac ccttatgctc ataaaaacgc tcatcttcta ttgcaattac agcatgttga
    10921 agataagggg aaatcttatc taatggaacg ggtgtctttt tatttacact tggagctgta
    10981 gacaatgttt ttccattagc gtatttaata gtagttgttt ggttcgtctg tttatctaaa
    11041 acagcttctg taactggagt tacatctctt agtgacgcaa atactagacc agcagtacct
    11101 gctgtaccaa caactagtaa aactaaaaat actatcccaa atactcttag ttttttaaat
    11161 ttatcgcttt ttttagattt tttcttgttt tttgttgaac gagtcttgtt agctgaagtt
    11221 ctattaattg gtttatttgt attaccagat gagctaactt tttttctcct gattttattt
    11281 ccatttttat cattatcgtt attcataaga ttttaacctc cttacttaat gcaatattat
    11341 aacacaaata ggagtagttt tgtaattaat agaaaaaatg aatttgctat aatattctgt
    11401 acattgtata tgtataaacg taatatatta aaaagtctat atttttattt tatttatttt
    11461 tgacaaatat tataaa
//
LOCUS       DAEPLF010000109        11393 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEPLF010000109 DAEPLF010000000
VERSION     DAEPLF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.109"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            292..993
                     /locus_tag="KR314_002357"
     CDS             292..993
                     /locus_tag="KR314_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBH0328735.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKSALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLLVVFILGHRMRGIVEKMNVKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAVISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIVLS"
     gene            1019..1675
                     /locus_tag="KR314_002358"
     CDS             1019..1675
                     /locus_tag="KR314_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328736.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGLRFGQMPMLSVIFQI
                     WGLVATTYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFITDSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDSNKTKE
                     "
     gene            1882..3522
                     /locus_tag="KR314_002359"
     CDS             1882..3522
                     /locus_tag="KR314_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBH0328737.1"
                     /translation="MFDVKIANGIIIDGTGNSRYKGDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPIKLNLHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDKVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWDGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            3881..5203
                     /locus_tag="KR314_002360"
     CDS             3881..5203
                     /locus_tag="KR314_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328738.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMLKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDICCC
                     ITGLEEAYHAMKKENIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPAVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAI
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            5400..6407
                     /locus_tag="KR314_002361"
     CDS             5400..6407
                     /locus_tag="KR314_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBH0328739.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGTMGCPPEILKKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            6445..7842
                     /locus_tag="KR314_002362"
     CDS             6445..7842
                     /locus_tag="KR314_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBH0328740.1"
                     /translation="MASTNKNNVTSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKINTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIFKGEDN
                     MSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKITHNIWE
                     EKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIWSNFGA
                     DGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDNLWIVT
                     QLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEMMLEMT
                     SRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYEVERVT
                     CNQREKNEGYDVSIRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKEEWITF
                     DGMDVLKQMYMGVEE"
     gene            7987..9252
                     /locus_tag="KR314_002363"
     CDS             7987..9252
                     /locus_tag="KR314_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0328741.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTDIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            9294..10333
                     /locus_tag="KR314_002364"
                     /pseudo
     CDS             9294..10333
                     /locus_tag="KR314_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
     gene            10368..11390
                     /locus_tag="KR314_002365"
     CDS             10368..11390
                     /locus_tag="KR314_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0328742.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMVAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
BASE COUNT         4106 a         1297 c         2286 g         3704 t
ORIGIN      
        1 ataacaaagt tttttgatta aaagttttat attgaaatgt caaagtaata aaatacctta
       61 taagttaatt acaggttaaa aaaataatat gtgaattttc tgatatttac taattatcaa
      121 taaagtattt aagtattaat gtttttagtg agactttaag acagataatc tcctattaaa
      181 gatactatat cttagataaa ataaattaaa acaatataaa aacataacaa gattacagaa
      241 agaaagctca taataaaaca ctaaaaaatt tattttaagg aggaataaag tatgaagttt
      301 caagaaatga ttaatagccc aggtctttgg attgtttcta gttttttagt aattatttca
      361 gttgttcaag caatcgtatt tatgaaatca gcacttaaag aggcttcaaa tcttggtatt
      421 gaaagaaaag gaattaaagc agcaattcgc tcagctagtg ttacagcaat tggaccctct
      481 ctatctcctg ttattacatt attatcactg gtagctgtga ttggtgctcc tacgacttgg
      541 atgaggcttt gtgatgtagg agctgccaga acagaacttg gagtaatatc tcttacatca
      601 aatttatctg gagtagaagt aggtagtgct gcatttggag cagaagcttt ttcgtatgca
      661 ctatggggaa tggctttaaa taacttaggt tggttgcttg ttgtatttat tttgggtcat
      721 agaatgcgtg gaattgttga aaaaatgaat gtgaaatata atcctacttg ggttaaaaaa
      781 ctacttgcag gggctacagt tggattattt gcttaccttc tttcaaatca aataaaaaca
      841 ttcgaaatac caaaacttac accagcagtt atttcagcta tagttatgct agtattgacg
      901 actttattta aaaagaatca acgattacaa gaattatcct taggtattgc catgttgatt
      961 ggtatgtttg gcacacagat tgtactatca tgattaaaac agataagagg tgataattat
     1021 gtatgataaa aagatgataa aaatagggag aatttctata ggtcttgcaa ttattgcaaa
     1081 ttttattcct gcaatctatg taggtcttcg ctttggtcag atgccaatgc tttctgttat
     1141 attccaaatt tggggattag ttgctacaac ttatggagtg agttggattg ttcaacctat
     1201 agcttattat ccaacactag gagctccagg ttcttatata ggttggcttg caggttctgt
     1261 tggagatata cgtatgcctg cagcatcaat ggcacagaaa attgcaggtg tagaagcagg
     1321 aactcatgag ggtgaagttg taggaacaat tggtacatgt tgttcaatct ttgtatcagc
     1381 ttctatgatt actattttta ccattgtagg ctataaagta attcctttgt tacctgaatt
     1441 tattactgat tcatttaatt atatacttcc agcattgttt ggggcaattt acgttgatat
     1501 tgcgcgtaaa gatattcgag ctggtggatg tacgattgct gtagcattag ctattatgta
     1561 ctttggtgaa aaaattggta taccaggtgg attgttgaca ttgtttattg tgttaggtgg
     1621 gatacttatt acaagggttt tctttgtaat tgatagcaat aaaacgaaag aataggattg
     1681 tttactagga agaaagacta gaattatttg ctgagaaaaa gaataggatt gtttactagg
     1741 aggaaagact agaattattt gctgagaaaa agaataggat tgtttactag gaggaaagac
     1801 tagaattatt tgttgagaaa aaagaatagg attgtttatt aagaaatatg aatataaata
     1861 agtcttgaaa ggagaagaag aatgtttgat gttaaaattg ccaatggaat aatcattgat
     1921 ggtacaggaa attcaagata taaaggtgat gtagggattg taggagacaa aatcatggct
     1981 attggagatc taagtcaaaa agaggctaaa gagactatag acgcaactgg aaaatttgta
     2041 tcacctggtt ttatagattt ccacactcat tctgatttat ctcttgtgta tgacaaatac
     2101 acaagaagcc gtatccatac aggtgtaaca acagacgtta ttgcaaactg tggtatagga
     2161 gttgctccga taagagaaga gaagaagcag gagttaattg actatcttgg aacgaggata
     2221 attggtacaa ttccaataaa attgaacctt cactggaata caatgcagga atactttgat
     2281 tacttaactg aaaattctcc agcagtaaat gttgtagctt atgtagcaca aggacctgta
     2341 cgtatcaatg aaatgggatt ttcaaaggag ccagctactt tagagcaact taaaaatatg
     2401 aagttggaag ttagaaaggc tatggaagct ggatgtgtag ctctttcgtc aggattagtt
     2461 tatttaccag gagcatatac aaagaaagaa gaaatggctg aattatgtaa ggaattgata
     2521 ccatacaatg gatactatat atctcatatt agagatgagg gtgatgaaga aatggaagct
     2581 cttgatgaag caatttatat agctaagacg gcaggagttc ctctacatat ttctcattta
     2641 aaagtcatgg gtcataagaa ttttggaact attgataagg tatttaaaaa gctagatgaa
     2701 gctgaggcag atggcttgga agttactttt gattgttacc catatacagc tggaatgaca
     2761 tctttgggtg cacttttacc tccttgggct tttgaaggtg gtgtagaaaa catggtaaaa
     2821 cgattagaag tgcaagaaaa tagggacaga attataaaag agctagagga agggattcct
     2881 ggatggcaat gtttctacca gttagcaggt ggttgggacg gtgttgtact agcctctgta
     2941 atgactgaag caaataaata tgtagagggt aaaactttaa tggaggtagc taaaatcaat
     3001 ggtgaaaatc catttgatac gttctttagg cttttgattg aagaaaaaag taaaattcaa
     3061 gtagttgttc atacacaagg tcaagaggat acagataagg tcgtttgcca tccaaagtca
     3121 tgtataggtt ctgatagtat ggacctttct acagaaggct tactttctct tggaaagcct
     3181 catccccgtg catttggtac ttttggacga attttctctt actatgttag agaaaaaggt
     3241 atgttgactt ttgaggaagc agttaaaaag ataacttatc tttcagcaaa acgcttagga
     3301 atttataaag aaaggggtct acttaaggaa aattattttg ctgacattgt ggtatttgac
     3361 ccagatacaa tagaagataa agcgacttat agtaatccta aacagtatac agtgggagtg
     3421 gaatatgtat tggtaaatgg taaaattgca ttggcaggag gaaagcaaac tgatgtatgt
     3481 gctggacgtg taattaaaaa tcctcttagc atagcaaaat agaatacatt aagaactttt
     3541 ataaaaatag tctttctttg aatattaagg aaggctattt ttgttaaatt aataaagatt
     3601 actctacttg tgttttctag agaatttaac tttaagtaga aaaaacaata tattaattgt
     3661 aaaatgtaat taatattaat ggataagatt taaatacaaa aacttaaata caaaaatgtt
     3721 ttaacagata gtcataatta aaaaacttaa agtaactttt tatataagat ataattgatt
     3781 atgcaaaatt ttatgaagca gcaaccttgt tactttaagt caagggaggt tcagaaagtg
     3841 tcttaagaga taatagtatc tttaggaaag gagaaacgaa atgaaaatac tttatgtagg
     3901 accaggcatg gtaatcgaca aaatactagc agtaagtaaa aagaattttc ccaatattga
     3961 agcagatttt cttagatatg atagatatat tgaagtacct gatttattgg aaaaatatag
     4021 aggtagagta gatgcagttc tttttacagg taaatcgcca tttaaatttt ttgaaaaaag
     4081 acaaaaagat tttgtgttgt ctgattatat tcctagacat gaaacaacat tatacagagt
     4141 attgcttgaa attacatatt tgcttaaatt cgatattagt agacttagta tagatactta
     4201 tgataaagct atgctgaaga gattgtataa agaaattgga gttgaattta aggatgaaca
     4261 tatgtttttt gcagagcaaa agtatttaga agaggactac ataaagtttg tgcttgaatt
     4321 tcataaatat aattatcaaa ataatgacat atgttgttgt attacaggtc ttgaagaagc
     4381 atatcatgca atgaaaaaag agaatattcc agtagtttta gccttgcctt ctgaggatgt
     4441 aatggtacaa agtataaaaa acattcagat gcgttatatt gcaaagaaaa acaatgagaa
     4501 tcagattgtt gttcttgcta tcaaacttaa tatgccttca gaatattcac tgatgaagga
     4561 agatgagtac gcttatcttt cacaaaggat taagattctt gataaactat accatttcaa
     4621 tagccgtata gatggtgttt tggtagagca gagtagaagc gaatttatga tttttacaac
     4681 taaaaaaata cttgagttag aaacaaagaa gtacagagat atttatttgt tggatatgat
     4741 tcgtgaagtt tcagttataa acacttatat aggaattggt tatggaaaaa cagcaaatga
     4801 agctaaattt aacgcttatg agagtattaa gatggctcaa agtcaaaaaa atgatgcagc
     4861 atatgtagtt tttgaaaatg gagaaattat gggaccacta aagccaaatc ctgctgtcaa
     4921 agagaaaaat agctttgatg aacgatttta tcaaattgct acagagacag ggctaagtgc
     4981 taatactgta tataaagtgt tcaataaaat aataaatagt ggaaaaactg aatttacatc
     5041 aaaggaatta gctattatat gtggtgtaag tattagaact atggacagga ttatacttag
     5101 attgtgtgat tctggttatt gtgaggtagt gagtgaacag cttatgagca agtatggtag
     5161 acccagtaga atattacgtt ttaaaccttt tctgttgttt taaatagata taagttaaaa
     5221 attaaatttt tatgataaca ttttctttac aattttttag atatatgtta atatttagaa
     5281 aaatgcatat gtattcataa aataaactca aaatagctaa aaaatcatta aaaaggaaat
     5341 cgtatgcata aaatgagaaa gttaatttat aacttaaata aatctgggag gagataatta
     5401 tgggaaaata tcaggattca aaaaaagttg taagaaatta ttttgaagca ttagaaaatg
     5461 ctactgggga tgaggttgaa aatgtcttaa agcaacatat gaaatcagat tataactgga
     5521 aaggtgtgta tccatttcgt gaacaagaag gcactgaaaa tgtagcagat gtattttgga
     5581 agcctttaaa aaaatcattg agtaatatgc aaagacgaca agatattttc attgcaggaa
     5641 caaatgagat tgacaatagt gaatgggtaa tgagtatggg gcattttatg ggactttttg
     5701 ataaggattg gcttggcatt aaacatactc gtaaaatgat tagtttaaga tatgcagaat
     5761 ttaactgtgt tcaagatggt aaaattgcta aaactggatt atttgtagat ataattgggt
     5821 ttatgattca agcaggagtt aatccattgc caccacaaac aggttcatat tttgtatatc
     5881 cagggccaat tgaccataat ggattacttt ttgaagatgc agatgaagtt gaaggagtaa
     5941 aaactttagc attagtaaat aaaatggtag atgatttatc tgaattaaat gaaagtggta
     6001 ctatgggatg cccaccagaa atacttaaaa aatcttgggc taaagatatg atttggtatg
     6061 gtccaggtgg aattggtgct tcttatacta tacctagata tcaagagcaa caccagctac
     6121 catttagaaa taatttaaaa ggaaagactt tcaatgggca tgtatgtaga tttgcagaag
     6181 gtaattttgc ttgtttcttt ggatggccta atctgactaa tgtaccatgt ggaggttttt
     6241 taggattacc aggtggagag gttaaagcag atatgcaagt tgttgatgtt tattatcgta
     6301 agggcgataa acttcaagaa aactgggtgt taattgatat tccttattgg ttgaaacaac
     6361 aagggcttga tattcttgag agaactaaaa gtatttttaa tgcttagtta tgcatacgta
     6421 ttctaaaaca aagggggata taaagtggca tctaccaaca aaaataatgt aacaagtaaa
     6481 gaggaagaaa aagaagtttc tacaagtggg cttgaatcat gtacggttaa agaaaacaaa
     6541 gctaaaatga atcaaactaa ttttagtaat ataaatgaaa atcaaacaaa tgagaataaa
     6601 acgaacacat ataaaggaaa tatagataaa ataaacacaa ataaatccga cataaataaa
     6661 gttaacatag atacagtgga tacagataaa ttaaatacaa atactcttac tgaaaaaaat
     6721 aaaaaagtta tatttaaggg ggaagataat atgtcatcta caaaaattta ctatggagat
     6781 tcattagaag taacgcctgt tggagtaatg gattacaatg atttttcaaa acagacaaaa
     6841 agggaacagg aaatacctgg atttgattct aagtatagag attttgtaga ttatattatg
     6901 aagattacac ataatatctg ggaagaaaaa ggtataggtg ttatttatga tacatatcac
     6961 aataatgtta caatgcattg tggctcttca aatttagttg gtattaaaga tgttatttcc
     7021 aatactcttc aaacattaca tgcgtttcct gatagaagat taattggtca aaatgtaata
     7081 tggtctaact ttggtgctga tggtttttta tcttctcacc gtgttttgtc tacagcaact
     7141 aacttaggtg atagtaattt tggaccagca acaggtagaa aaataaactt tagaactgta
     7201 attgattgtg ccacaactaa caatagaatc cacgaagaat ggttggttag agataattta
     7261 tggattgtta ctcaattagg attaaaccct caggaagtag caaagagtat ggcaaaggca
     7321 agtgaatcta aagtgcttag cttacaatca acttatggta tctgtgaatc tatggatggt
     7381 caatttatgc caactaagta tcaagcaaaa gatgattctg ttggcgaaat gatgcttgaa
     7441 atgacaagcc gtatttataa ttataaatat ataaatgaag ttaaaaaata ctatcatgat
     7501 aatgcagtag tacattttat atgtgataaa gatttaaatg gttatgatga gattcaagga
     7561 atgattgtaa gtttacttgc atcaattcca aatggaagtt atgaagtgga aagagtaact
     7621 tgtaatcaaa gagaaaaaaa tgaaggttat gatgtatcta taagatggag attacgtgga
     7681 ataaatgaag gtataggatt ttttggacag ccttctggaa agcatataga agtaatggga
     7741 atcaatcatt atcatatact tcaaggtaaa gtaaaggaag agtggataac ttttgatggt
     7801 atggatgtat taaaacaaat gtatatggga gtagaagaat aaagctgttt agagtataaa
     7861 taattttgaa atgtatatag ggaagtattg ctttataaaa tttaagtggg gaatgtatat
     7921 gaaaaataga tgtttaaact tttagcaaaa attatgacta aatttaaaag ggggaattat
     7981 aaaaatatga aaacaacggt tgaaatagaa aatgatatca tattaggaaa agactattca
     8041 aacctaaaaa gaggaattgc atttttttca attgctttaa tatacttttt ttattgctac
     8101 aattttatgg tgggtacttt tattaaacca acaatgattt atgctttagc agatggggga
     8161 tttggctttt cattaaaaca aacagaagaa atatttgcag ttatgtcttt tggtacaatt
     8221 ccaggtacat ttatttttgg ggtgatttct acaaaaattg ggaagaagag gactttaatt
     8281 agcgttgctt tactgattgg attgacaaca tttataccaa tgttatctcc tacagatatt
     8341 atgttatgga aaatagcacg tttgtgtaca ggagtagtgt tgggaggcgt atttggaaca
     8401 gctatgcctt tagttgcaga tatgtttcca agtaaatatc gtggtaagtt agctgctata
     8461 ttaactgctt tattttcatt agcaatgatt tttggtggga aagtatatgg cgttttaggt
     8521 gatgcaaatt ggcaaatatt gatgtatact gcgattatac cacctatagt tggagctatc
     8581 ttagcaatct tctttgtgcc agatgatttg gagtatacaa aggaattagt taaaaaggga
     8641 aaagaaactg gtgaaaagat tagctatata agtatgtata agggaaaata cttatggatt
     8701 ggattgggaa cgattctttt atcaggtgca aattttacag catattcagc attttcaaat
     8761 aatgcaacta catacctagt gacaggaata ggaatgtcag cagcagtggc tgggtctata
     8821 tatagtttac aaggaattgg tcaattagta ggatatttat tctggggttc aattgcagat
     8881 aaatttggaa gaaaaatacc ttttataggt atggcattag cagcagtatt tgtttttata
     8941 tatacaaaat taggtggaga taatacaaca acattctata tgatatcagt attactaggt
     9001 gttgctgttg gatattctgg agcatgggga gcatactaca cagagttgtt tccaagtaaa
     9061 tatcgttcgt tgtcatctgg tatgtcattt aatggaggaa ggattatatc tacgtttgca
     9121 atacctgcta tagctggtac agcttctggt actttaggta tgatgactat cttttacata
     9181 tcaatcgcag tctttgtagc aggtgctatt gtatggttat tcttaccaga gacgttaaat
     9241 aataaaaaat aaaatactag gaatttattt tgttacttag aaaggggtgg cttatgattt
     9301 catcaattga tgtatgtatt ataataggat atttgctatt aatgttagtt attggttatt
     9361 attcaggaaa agataataaa aatcaagaag attatttctt ggcaggtagg tcaatgccat
     9421 ggattccaat tgctttatca gttgcagcta ctatgattag tgcaaatggc tttataggtg
     9481 gtcctggatg ggcatatgta gatggaatgt atccagtaat gtaaaatgtg caaaaaattc
     9541 ttttgtagtt agtgcatttg ttatgaattt agtttactac tttatgctat tggtaggagt
     9601 aattttattt gttttttatg ggggaaggac atttgaaact tcaaatgaaa taatgattac
     9661 ttttatttta aatgaacttc cagtaggagc cattggaatt attattgcag gtgtgtttgc
     9721 agcagcaatg tctagtatcg actctatatt aaacagtatg acaactgttt ttacgaagga
     9781 tatttatgag ttttatttca aaaaagacaa caaagaatca tcattaaaag ttacaatgat
     9841 tataactgta gttataggag ttgttatgac aggttttatt attatgggat ttggaggaag
     9901 catcaaatca attcttgacc ttgttggtaa ctatatctca tactttgctg gaccagctac
     9961 aggtgcattt gtactagcaa tgtttacata taaagctaat gataaaggag tatctattgg
    10021 ttttattgta gggttagttt taggattttt tatttctaat aaatataatg tgagttggtt
    10081 gtggaatcca gcaataggtg ctacaataac attgatattt ggatatattt ttagtgtcat
    10141 tttaaagaaa aatcataaag tagatgatat taaaaaatac actgcatttg gtatgagaaa
    10201 tcaccttatt tctcaaggaa tggataaagt agatggagta tctatagtgc cattttcttt
    10261 aggtaaacaa gaactaattg tattagcttt ttttattatc caatatatta tattattttt
    10321 gatacaattt taagaagaaa tactagatat ttaaggagaa atactaaatg aatcaaaaga
    10381 aagttcatta taataaatcg gctacatcaa aagatgtcgc aagattggca ggagtatctc
    10441 aatcatctgt ttcaagagca tttggtagtg caaatggaaa gggcgtaaaa cctgaagtaa
    10501 gagaaagaat ttttcgtgta gcaaacgaaa tagggtatgt tcctaatttg gtagcacggg
    10561 gaatgattag tggaaaaact aatgttatag gtttgatagt tggtgatagt ctaggtccat
    10621 tttacaatag aattatcaat ttatttgtag aaaaaattca agagataggt aaacagtgtc
    10681 tcgtttttaa agttcctaga caacaaagta tagatagtat aatttcaaaa gtaattcagt
    10741 ttcaagtaga agctgtgatt attacagctt cggccatgaa taaggtgatg gctgagactt
    10801 gtgaagaaaa caatattcct gtgattcttt ttaatcgttt tatacctggt ataaaaataa
    10861 gtacagtcta tgtagaccca attgaaggtg ggggcatggt tgctgaatat ctgcttaaga
    10921 aaggtcataa gaatatagga tatattcaat ttacgagaga aacaagtgaa gaaatggaaa
    10981 agaaaattgg attttattct aagttaagac aaagtgggat tcataacctt aaagaagaat
    11041 ctgcaaacta tgattatgat tctggatatg aagcaggtaa gagaatgtta gcattatcaa
    11101 atcctccaac agcaattttt tgcacaagtg acttgatagc tattggtgta atggatgctg
    11161 caaggtttga gtacaagttg agggtgccag aggatttatc agtgatagga tatgatgata
    11221 ttcaaatggc ttcatggaag agttataatt taacgacgat tagacaacca ctagatttgt
    11281 tgattgaaaa aacaattaac atacttaagt ccttattaaa tgaagaaaat ccagagtcag
    11341 tcgttgaaat gttaaaacct gaattgattg aaagaggttc tacactttaa gtg
//
LOCUS       DAEPLF010000110        11391 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEPLF010000110 DAEPLF010000000
VERSION     DAEPLF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.110"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(231..800)
                     /locus_tag="KR314_002366"
     CDS             complement(231..800)
                     /locus_tag="KR314_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0328743.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            1003..1230
                     /locus_tag="KR314_002367"
     CDS             1003..1230
                     /locus_tag="KR314_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH0328744.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(1449..2192)
                     /locus_tag="KR314_002368"
     CDS             complement(1449..2192)
                     /locus_tag="KR314_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBH0328745.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYITDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            2360..3232
                     /locus_tag="KR314_002369"
     CDS             2360..3232
                     /locus_tag="KR314_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0328746.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            3232..3897
                     /locus_tag="KR314_002370"
     CDS             3232..3897
                     /locus_tag="KR314_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBH0328747.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            3918..4775
                     /locus_tag="KR314_002371"
     CDS             3918..4775
                     /locus_tag="KR314_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0328748.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGSYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(4931..7477)
                     /locus_tag="KR314_002372"
     CDS             complement(4931..7477)
                     /locus_tag="KR314_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0328749.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(7477..8163)
                     /locus_tag="KR314_002373"
     CDS             complement(7477..8163)
                     /locus_tag="KR314_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328750.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(8246..9235)
                     /locus_tag="KR314_002374"
     CDS             complement(8246..9235)
                     /locus_tag="KR314_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0328751.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIVCEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(9222..9908)
                     /locus_tag="KR314_002375"
     CDS             complement(9222..9908)
                     /locus_tag="KR314_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0328752.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(10408..>11391)
                     /locus_tag="KR314_002376"
     CDS             complement(10408..>11391)
                     /locus_tag="KR314_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBH0328753.1"
                     /translation="KNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGD
                     STTMYPSQNISDEIKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRT
                     VPYISKVSGVPIVDLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEV
                     SLGPEMKSTGEVLGVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAK
                     DMKELGYTFVATEGTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKG
                     NDSTRDGFKMRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         3976 a         1914 c         1620 g         3881 t
ORIGIN      
        1 taaatagatt tttcaagatt tatcatcttt tcataaatat attatagtta aatattttaa
       61 tattacctaa catctacaat aaaatatact cttaaaataa aattatataa ttaaaaatac
      121 tctataaaag ctaaaaagtt ttctattcgc tacatctaaa aataattaga tagaggtata
      181 aatgtattta gtttatttat acctctagtc ttttataaat tatatctcta ctaaattgat
      241 tttttagcac gaataagtgt gctcttggag ataccagtca tcttcacaac ctctctatat
      301 ggcttagaat ttaataaccc caatgcatat tgtatctgtt gattgctgta tttctttggc
      361 ctaccatctt taaagtcctt tttcttttta gcaaattctt ttcctaattg agtacgttct
      421 attatcatat ttctctcaag ctctgcaact gcaagtaatg tagtcaaaaa gaatttaccc
      481 attgaagtat tttctaaaag acctatattt aaaatatgaa cacttacttc tttagcaaat
      541 aaaccttcta ttagttgtat cccttctgat gtacttctag ctagtctatc cagcttagtt
      601 gcaacaagta agtctccctt ttttagagat gatataattt tttcaaattt aggcctgttt
      661 gtagtagtgc ctgtataagt ttcctttatt attttagcat cattatattt attcaaaatt
      721 tcttgctgtt gctgatacaa actgttgttg tctgtttgat tttttgtact tacacggcaa
      781 tatccatata taataatcat cacttcctaa cataaagatt ttgtacagaa gttacccaga
      841 agcttgtttt tacttatctt tatccatttt actaaaacga tttttgggtg tcaatactgt
      901 taacttttga caccataata attcctttat aatttgtttg actatgtcaa aaattaccaa
      961 tataatgaaa ttaatgagtt tatacaaatt ggaggaaata atatgaatag tctgttaaaa
     1021 gaagcaatag atgaattaaa aaacatccat tgcggggaag tttttttatt aaaagaattg
     1081 ttcaaaggct atgaatggaa caggttggag atgaatatta gattgaaatt aggaaaactt
     1141 tttttgtatg aagtggtaac taatcccaat ttaaatgtag aggtcatcaa taaaaattct
     1201 gcttatcagc aaatgtataa aaaaaaatag tgacttaaat gtatataata gtataatctt
     1261 cttattgatt caaaagttaa aacttttaat acaaaacaat cattctattt taacatttat
     1321 caatatgtct aaatttatat aattaacaca tacgaatagt attgtttaaa tcacattatt
     1381 taagatagat aaatcttaac cctactccag cataaatact aaaatagggc ttagaattat
     1441 ctatattttt atcccataaa atgcttagct cttcctcttg attgaacata tatatctggg
     1501 gaaatacaat agaagcgtaa atttataaaa tttccaaagt cagaaaactt cagttcaaac
     1561 ttaggtatac caaaactttc aggtgctatt tcatcaacgc caaaatagat gataattcca
     1621 ttatctgtaa tataaaatgc ttggtcttca ggtataacta actcaacatt agaataatac
     1681 atttctctat tttcatttat ttgagattta atgtaatcag taattatatt aatataatca
     1741 actccatctc taaaaatgtc attaagtaaa agttcttttc cagttgtcaa atctatattg
     1801 taattattca atgtattgta tttaggccca tagttattag caaaccccat aaggtttaat
     1861 atcatactca gtacataatt tttattaaaa gtaactgcgt aactggtaga tacccaatag
     1921 ctttgttttg gaagtgaatt ttgagaagct gcgttattat attctcgttc ttcttcctgt
     1981 aagccatttt taaaattatt tacagtcatt cttacagcat tgtttatgtt atttaaaata
     2041 actgggttta catacgtgga ttgatttcta atattgtata taacaggata ctttacatca
     2101 tatgaaaaat acttacttct ttcaaaatct tcattaaatt gtacatataa tatattatat
     2161 gtacaggtat ttgtagattg ataattttcc attaaaatcc tccccctttt ataaaaaaat
     2221 cagatacaat ataatatgct taaaacagta ataatgttaa tagtaaatgt aaagaataga
     2281 aagtatctaa aacagcaaaa aatgactaat tttgtggtat aatacatagg attgagagtt
     2341 attttatagg aggaaaaata tgttattatt agcagataaa tggaaagatt atgaacttat
     2401 agatatggga aatggagaaa aattagaacg ttggggaagt tatgtactta gaagaccaga
     2461 cccacaggtt gtatggccaa tggaatctga atgggcttta tggaaaaacc ctcatggaca
     2521 ttatcacaga agcaataaag gtggaggtca atgggaacac aaaaaaagat atccagaaca
     2581 atggactata aattataaaa acttaaagtt tcacattaaa ccaactggct ttaaacatac
     2641 tggattattc ccagagcaag cagctaactg ggattggatg atagagaaaa taaagaaagc
     2701 taacagacct ataaaagtgt tgaatctttt tgcatataca ggtggagcta ctgtagcatg
     2761 tgcatctgct ggtgcagaag tatgtcatgt tgatgctgca aaaggtatgg ttaattgggc
     2821 aaaagaaaat atagaattat caggtcttaa aaatcaaaca gtaagattta tagtggatga
     2881 tgttgtaaag tttgttgaaa gagaaataag aagaggtaaa aaatatgatg caataataat
     2941 ggaccctcct tcttatggaa gaggtcctaa aggagaagta tggcaaatag aagaaaagtt
     3001 atacagtttt gtagatttat gtatgggagt tttatcagat aaccctttat ttttcttaat
     3061 taattcttat acaacaggat tttctccaat agttcttgaa aatgtattgt ctacaacaat
     3121 aggtaaaaag acaaatacag gagatatata cggtggggaa ttaggaatac cagcttccag
     3181 agatggtaag atacttcctt gtggtatatt tggaagatgg gagaaaaaat aatgataaaa
     3241 gtcatatatg aagataacca tttattagtt gtagaaaaac cagttaacat attgtctcaa
     3301 ggagataata ctaatgataa agatatggta aatcttttaa aagactatat taaaaaaaag
     3361 tataacaaac ctggaaatgt atatattggt ttaatacata gattggatag accagtaggt
     3421 ggagttatgg tgtttgcaaa aacatcaaaa gcagcatcta gattgtctga gcaggtaaga
     3481 agtaagactt tcaaaaaaac ttatctagct gttgttcatg gcaagataaa atctaactca
     3541 gatatactaa aagatttttt atataaaaat aaaaaaacta atatggtaac tgtagtgaaa
     3601 aaaagccata aagatgccaa agaagctgaa cttaactata atgtattaga ttttaaagaa
     3661 aatctaagtt tagttcaagt taatttaaag actggtcgtt ctcatcaaat aagagttcaa
     3721 tttgcaagca gaaaacatcc attatacgga gaccaaagat atggcaaaga aataaataag
     3781 attggacaac aaatatctct ttggtccaat aagatagaaa tttgccatcc tactacaaaa
     3841 gaaaaaatgg aatttacatg tcatccacct gacaaatatc catggaatat attttaaatc
     3901 tcaaatgagg tgattatatg agtaaaaaat tttcaatagg agaaatgtct aaacttcata
     3961 aaataagcat acagacacta aggtactatg accaaataga tttatttaaa cctgttcatg
     4021 ttgaccaaga aagcaattac agatattact caattgacca attttcacaa ttagatatta
     4081 taaaatattt aaaatattta ggtatgcctt taaaagaaat taagtcaaag cttgatgaaa
     4141 ataatgaaga aattctaaaa ttactaacaa ataaaattaa tatagtggat gaaaaaatta
     4201 aagaattaga gcttataaag aaggtactaa gcacaaagaa agaaactatg gaaaaaggta
     4261 tgaaagtaga tgaagttggt aaaatagtga gaaaacatat tccacgaaga agtatcttat
     4321 ctatagagta tgaagaagga ttaagtagtg aagaaactat ggaactttcc agaagacaaa
     4381 tagcaaatat attagaagat aatatatctg tgttctatgg tggagtaagt ggacttgtat
     4441 ctatagataa atttttaaaa gaagataaag ttatttatga caatagtttt gttactgttg
     4501 aaagagactt atttgatata aaatcacaaa aacatataac agaaatttca tctggtgatt
     4561 ttatttgtat gacatataaa gggtcttaca aagaaaatta taccgctttt aagaaactgg
     4621 ttgattatgt tgaatataaa agaataccta ttgaaaatta tatatatgaa atacctataa
     4681 tagaccctct gtctacaaac aacgaagagg aacttctaac agaaattcaa atacaagtta
     4741 aaaagagaga aattgactta agatgtctag tgtagtaatc tagtttactt acacatctta
     4801 atatagttgt ctattatcaa catatgattt ttctagatag tgattcctaa cagtgagatt
     4861 ttcaaaaaat aggaggaaat atggctaatg ctaattccct cctattttta attaatccat
     4921 atcattttaa ttaagccatg tcatcacgaa gtgcatcaat aatattttct ttttttatct
     4981 tattaatagt atacaacatt gtaataaata ctataaataa aacactaaat atactaattc
     5041 caatactaaa ccatggaatc acaaaatcaa tatcatctgt aaccattact ttatgaatta
     5101 gccatgaaga aattactgct ataggaagtc caaagaacaa tgctctcatg ccataaaaag
     5161 cacactcaaa attcatcatc ttttggaaat catgctcaga cattcctaca gagcgaagca
     5221 tggcaagttc tctacgacgt aacttaatat tagttgaaat tgtattaaac acattggcaa
     5281 tcgaaatcag agaaatcata attatgaaag tataggcaaa aacattggca ataaaaatgt
     5341 aattacggtt ctcatccatc attccataca tattgtacag attgtactca ttagtaactc
     5401 cctcactctc aataatttgc tccatttctg caactgactg agtaggattt ttcgattgaa
     5461 agcttaaact ttcaattcct gtagaaacac caggaatctc aaacttatct ttggctgaat
     5521 aaggagccaa tactctgaag cttgctttct tcacatctga acttctgatt cctggaggag
     5581 aatcaggtgg cactactttg acaaatgaaa tattcacatt ttgtctctca ggtatctcca
     5641 acttgccatt agtttcagga gtgagactaa cattcataga agaactgttg aaaatatcaa
     5701 taatattatt atcctttgtt accatagaaa cagcaatcag ttttgcattt tgtccagtat
     5761 attcttctgc tggtaatcca agacttttaa taatattcag ataagttcta tcatcaagaa
     5821 attgcatatc tactggtaat tcaagcattt cactcataga atgtgaaccc tcatagttca
     5881 aataatcgtc tgtaagctca cttactttgg caacacatga ataggtcata agtttctgat
     5941 acaaaccttc gtaaacacca tttgcagttt ttagcttatc gtaaagcttc aacatttcgt
     6001 tgtctttcat accttgtgta gaaaaaataa tatcctgatc agtaacctct gacctctgat
     6061 ttgacatctg ttttaggtct gctactaaag cacttgtaga tataaataaa actatgctta
     6121 aaacaagcga caatacaata cttcgataac gtttcttgtt tcttttaaag tttttcatag
     6181 caagaactcc ctcaagacca taaactcgct gtgttagttt tgatgtcttc acagatttag
     6241 attcaatttt tacctcattt gtctgacgaa tacactccat cactggtgtg ttggcagcct
     6301 ttcttgctgg tatgtaagca gaaattaaga ttgtaaccat acttaccact gctgcaacga
     6361 tgattgcagg gatagacaat gttaatgtca acggaacgtt tacatagaga atatttgcaa
     6421 agtttttagc tatgacagaa atcacaagtc caatactgac tataccgatt gcaacaccaa
     6481 ttggtatacc aatcgcacca atgcaaattc cttcaaatag caccgaattt cgtaactgtt
     6541 ttgctgtagc tccaacagat gaaagtattc caaattgatg tgttcgctcg ttcaatgaca
     6601 tgttaaatga attatatatt agaaaaattg agcctagcat gattatggca agcacaatgc
     6661 caccaaccga gtacaaaaac atattgaaca aattgttatc agaaacacct atgaagcgca
     6721 acacattgtc attgaaaatg gaagcatggt ctttagtgct acttgtgtaa gagcgaatct
     6781 gatgtggatt tttcaatgtg acaaaaaggc taagactgtc agtcttgact tgtttatctt
     6841 ctttggttat caaggtatat cctggtgcag aagattcttc aaatacgggc tttctacaga
     6901 tacccacaac tgtataagtt ttcttatcct gtggcacaaa aacttcactc ccagcagtgt
     6961 aggggtcact ctgaccaagc ttttcatttt cactcattcg tttccctaca tccaaagaaa
     7021 tagtatcacc tactgcaaat ttgacaccac catctgtcgc aactttactt gaaacaagaa
     7081 tctctctact attttcaggt aatcttccag aaattaaagt tataggcaaa gtatcaaaag
     7141 cttctttgct aaatccagca ataaaaagat atggtttatc tggatttctg ttaccttcaa
     7201 gtgttgcata gccaatattt tcaaatgttg cagtatttgc aacttcatca ttgactcttt
     7261 gttcctgtac aaaagaagaa tccacatcca aaaactggac atgccaatca ccatatttta
     7321 taatagctcc atttgtcata tagttcatca aagaaactcc aaaggtagta actgctgtaa
     7381 tcatagcagc tgacagaaca actcctataa ttgttacaag agttcgagtg cgattctttt
     7441 tcaatccttg cagagcaact ttgttaaaaa tgttcattaa cgattcacct tcctctcatc
     7501 acgcaccact tttccatctg aaatacagat aattctatct gcctgtaaag caatgttttc
     7561 atcatgtgtg acaataatga gagtttggtg atatttacta tggctctctt tcagtaactg
     7621 gataatctct tgtccatttt tactgtctaa gctacctgta ggctcatcag caagcataac
     7681 tgctggtgca ttcatcaatg cacgaccaat ggcaacacgt tgctgttggc ccccagaaag
     7741 ttgatttggt aaatgtgttt ttcgttcttt taaaccaagt aattctaaca agtcatttaa
     7801 ccgttcttga ttgaccttac gcttatccat aagtataggc aatgtgatat tttccactac
     7861 atttaatgtg ggtatcagat tgtgaaactg ataaatcaat ccaacttgtc gcctacgaaa
     7921 aatagcaagt ttttcattgc tttggccata tacatcttgc ccctccagat atatctttcc
     7981 acttgttggt acgtctactc ctgcaatgga atgtagtagt gtagatttac cagaaccaga
     8041 agaaccaatg attgcagtaa actccccttt ttcaatagta agtgagacat ggtcaagtgc
     8101 agtaacctta ttttcattct taccataaac cttacataaa tcttcaatct ttaaaaacgc
     8161 cattatataa gtctcctttc atattttata aatatatcat agaactttca acttacattt
     8221 aagtgacatt taaatgagag attcgtcact ttggaaaacg aataacaaat attgcaccac
     8281 cttgtgggtg attttgtgca gtaattgttc ctccttgtct cataataatc gtcttacaaa
     8341 gtgccaatcc tattccataa ccagctgtgc ttgaattttt cccacgataa aatctgttaa
     8401 atagacatgg taagtcttct tttttaaagc ctgctccgtt gtcatgaata gtaagttgag
     8461 taaacaaaaa gttgtcctca caaacgatat caatttttcc attatcaccc acactctcca
     8521 tgcagttttt aaatatattt tgaattgctt ctgaaagcca atttaaatca cccaaaatcc
     8581 ttactccttt tggtatatca atatgtaaag tgatattgtg cagttccatg ggaatcaaaa
     8641 gttgatgaag tgcagaacta atcaaattat tcacatcaat ttgttctttt tgaaagacta
     8701 caatacctgc atctaaacgt gataatttta aaagggaagt aatcagccag tccattcgta
     8761 taagtaattc ctctgtttct cgtatcaact cttttctctc atcttcatca gaagtattct
     8821 ctaacagtga caaaataaga gttactgatg taagaggagt gcgaagctgg tgggctatgt
     8881 cagctagtga gtcagcaagg tgttcttttt cttttttcag tgcgtaattt tgctctctaa
     8941 tacgtaacgt cattttagtt atttcacttt gtagaataga aagctcaccc tcatctgatt
     9001 cagcaatata cagatggtta gcattatgaa gcacaagatt aatttgttct gaaatctgtg
     9061 caatactttt gtatcgattt ttagtaaaca caaaaaatgc tattccaaag gtagtggcag
     9121 aaacaataga aagtattcca gtcataatac tgattgcaaa tcccagggct acagtgagaa
     9181 tagtaattaa taagaataaa atagcaaatt ttcgaaactc tctatttcga aacataactt
     9241 ccccccaatc tataccctgt cccacgaaca gtcaaaatta tttgtggact tgaagggtca
     9301 tcttctatct tctctcgcaa gcgtttgatg tacacagtca atgtgttgtc attaacaaac
     9361 tcacctgccg catcccacaa ttcatcaagt aatcttaccc tcgtaataat acttttagga
     9421 ttgctaataa acaccaataa caaacggtat tctaaagctg aaagaaaaac ctcattgcca
     9481 ttctttttca caacaccggt tgccgtatct acataaagcc cacaaatttc aaaagttgac
     9541 ccaaaacgcc cactttttcg tagtgctgtt ctaattcgcg caatcagttc acgtggacga
     9601 aaaggtttag taatatagtc atctgcaccc atattcagtc cagtaacaac acttgattca
     9661 tcaccagaag ccgtcaaaaa gataacagga atgtcttgct tttctttgat ttctgtgcaa
     9721 atcgtaaacc catttccatc aggtaaagaa atatcaataa gtgccaaatc aaatttattc
     9781 ttagcaatct ctacaagtgc ttcacttctt gtggatacat gggttactat aaatccttct
     9841 gaattaagca aaagaataag atttttggca attgctttat catcctcaac taaaaatatc
     9901 cgtttcattt actttcatcc tcctttattt tattagtttt atatattcca gcagagcaat
     9961 agtacactac tattttactt atcattacac aattttcagt agtttctttt gttcttatat
    10021 aatccatgca taatacctcc attgatttat tatatttctt ataacataaa tacacaagat
    10081 aagacaaaaa acataatgta agtgcaagtt ttttaactca ctaacttata aacatacact
    10141 cctactaaca ctactttctt gatgacacat cttacaagct tatattcata catcaatgaa
    10201 aaattaaatt tatccattta ttaaaataaa aggcaatagc ttctatagct aatgcccttt
    10261 atcttaaaat gatttaaata ctatacaatt tttataaatg gctataaata aactcgtata
    10321 gaatataaaa tttatctaca gtcttttgtt atacaaaatt tataacctct tatagaacaa
    10381 atttaccact ttgtaaactt atcactttta ctcagctata ttataaactt ttaatttatc
    10441 tttgtttaaa tgtttcttct taacttctac taaagctttt agcgtatcca aagatgtcat
    10501 tatttcagta gaaaattcaa tggctgttct tctcatctta aatccatctc ttgtagagtc
    10561 atttccttta gttggtgtat ttattactat atcaacttgt ttatttctta tagcatctaa
    10621 tatgtttggt ctaggctctt caattcgatt tactatatca gcctctattc cattttctct
    10681 taaactcttc gctgttcctt ctgtagcaac aaaagtatat cctaattctt tcatatcttt
    10741 tgctatctct ataaactcat ctttatcatg attatttaca gtagcaagta ctactcctct
    10801 ttcatcagac atatgtctac ctgctgccaa gaacccttta tataaggctt cttctaaatt
    10861 ttctcctaca cctaggactt ctcctgtaga cttcatttca ggtcctagac ttacttcaac
    10921 tttagataat ttagacattg agaatactgg tacttttact gatactaatt ttggctcttt
    10981 gtaaactcct gtaccatatc ctaaatcctt taatttagct cctaacatac acttagtagc
    11041 caagtcaact attggtactc cacttacttt acttatatat ggaactgttc tactagctct
    11101 agggttaacc tcaattatat ataattcatt ttgaaattct ataaattgta tattaaccat
    11161 accaagcaca ttaaggtcta aagctatttt tttagtgtat tctactatct tttcttttat
    11221 ttcatcagat atattttgac ttggatacat tgttgtacta tctccagagt gaactccagc
    11281 tctttctaaa tgttccatta taccaggtat caatatatct tccttatcac atatagcatc
    11341 tacttctatt tctctaccag ttagatattt atctataagt actggatttt t
//
LOCUS       DAEPLF010000111        11209 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEPLF010000111 DAEPLF010000000
VERSION     DAEPLF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.111"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..389
                     /locus_tag="KR314_002377"
     CDS             <1..389
                     /locus_tag="KR314_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0328754.1"
                     /translation="AKSRYEVFACEQKIFSLNDYDDIDKSSYHIFLKENGLICAYARI
                     IPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIKINLHENNITLSAQTYIKN
                     LYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            716..943
                     /locus_tag="KR314_002378"
     CDS             716..943
                     /locus_tag="KR314_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0328755.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            969..3089
                     /gene="feoB"
                     /locus_tag="KR314_002379"
     CDS             969..3089
                     /gene="feoB"
                     /locus_tag="KR314_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBH0328756.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(3222..4040)
                     /locus_tag="KR314_002380"
     CDS             complement(3222..4040)
                     /locus_tag="KR314_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0328757.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            4247..4531
                     /locus_tag="KR314_002381"
     CDS             4247..4531
                     /locus_tag="KR314_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328758.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     repeat_region   4699..4992
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4766..4794
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(5653..7746)
                     /locus_tag="KR314_002382"
     CDS             complement(5653..7746)
                     /locus_tag="KR314_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0328759.1"
                     /translation="MNKRNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEQNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTILNKPIKINEFNEISNTKEGKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLIYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTQSHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
     gene            8399..9322
                     /locus_tag="KR314_002383"
     CDS             8399..9322
                     /locus_tag="KR314_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBH0328760.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESINLTKTKIY
                     SNLRDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDGLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLNKKDEDIYKILSLNLLKIIALGNDNSLKGTKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            9297..10124
                     /gene="panB"
                     /locus_tag="KR314_002384"
     CDS             9297..10124
                     /gene="panB"
                     /locus_tag="KR314_002384"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBH0328761.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGAKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIADMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGVFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            10143..10991
                     /locus_tag="KR314_002385"
     CDS             10143..10991
                     /locus_tag="KR314_002385"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBH0328762.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNINTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PKNIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         4126 a         1499 c         1463 g         4121 t
ORIGIN      
        1 tagcaaaatc aagatatgag gtatttgctt gtgaacagaa gatattttca ttaaacgatt
       61 atgatgatat agataaaagc tcttatcaca tatttttaaa agaaaatgga ttgatttgtg
      121 cttatgctag aataattcct aaagaataca gctcttataa tgatgtttcc attggtagag
      181 ttttagttct ttctagtcat agaagaaaag gattagcaaa acaaatgatg gattgtgcta
      241 tagattttat aaaaattaac ttacacgaaa ataatataac cttatcagca caaacttata
      301 taaagaacct ttacttatct tgtggtttta aagaaatctc agaagtatac gacgaagcag
      361 gtattgaaca tattaaaatg agattatagt gttgacaagc taatattgtt aatatataat
      421 ttaagtataa attaaatatt gaattgatat ttaggtattt gaaaatacct tacatagagt
      481 caggaggctt ttgctttctg acttttttat tatcaaaatt caataattat atggattcaa
      541 agtgttaatt tttattctat attctacttt ccccaaaagt aagaattata cccaactttg
      601 tatgataggc atcttgccta tctttttttt gtaaaagtaa caaggtaata atataatgca
      661 tagtttagaa taagaagatt agcccaggct aaatttcaag catggaggtg ctactatgaa
      721 aaaccttaac gatattcaag tgaaaaaaac tgtcaaagtt gaagatatat tatctagtgg
      781 taatttaaga gaaagaatgt tagcgcttgg tcttactaga ggagctgtta tagatgtagt
      841 tagaaaaggt ccaaaaaata atttaactgt ctataaaatc agaggtagta agatagctct
      901 acgacaagaa gaaagtagct taatattagt ttctgacatt taaaaatttc tagcaggagg
      961 atattaatat ggggttgaca cataactcaa ctaaaatgag ttctttaaaa gatatgtttg
     1021 atatagacaa taaggaagac cagtttgtaa ttgcattggc tggcaatcca aatacaggta
     1081 aaagtactgt atttaatcat cttacagggc ttagacagca tactggaaac tggccaggaa
     1141 aaactgtagc tacagctcgt ggtaatttta aatataaaaa taccgaatat gcattaatag
     1201 atttaccagg aacatattct ttatttgcct tatcacaaga agaaattgta gctcgtgatt
     1261 ttatatgttt tggtaatcca gatgcagtaa tagtagtatg tgatgctaca tgtcttgaaa
     1321 gaaatttaaa tttagtattt caagtgatgg aacttacaga taaagttatt ttgtgtatta
     1381 atttaataga tgaagctaga aaaaaaggca ttactattga taaaaaactt ttagaggatt
     1441 ctcttggtat tccagttatt ttaactgctg caagaaatgg ctctggtatg gatgaactat
     1501 tggatacttt aaatgatgta tcttttgaca aatacaaatt aaataataaa cctgttagat
     1561 acaatgaaaa tatagagaat gtggtaaaat caattcaacc agaattggat aatattatac
     1621 caggtataaa ttctagatgg ttgggactta gacttattga tggcgatgaa agtatatttg
     1681 aatctatgag taattatata gacaaagatt ctatagatgc tattaatgaa gtaaagaaaa
     1741 agattccaga caatataaat aaacagaaaa taagagatga gtttactaaa atcaactatg
     1801 attatgctaa aaaactgagt gatgagtgtt gttctaatgt tgctaaaaaa tcaactgata
     1861 gagaagaaaa agtagataaa atattaacat ctaaaatatt tgggcttcca ataatgctct
     1921 tattattagg tactatattg tggattacta tagaaggggc taactatcca tccactttat
     1981 tatctaattt attactcgga tttgagccaa gtatttcggg tatattaaat agtattaatt
     2041 gtccatcatg gttaaatgat atgttggttt taggattata cagaacatta gcatgggtaa
     2101 tctctgtaat gctacctcct atggcaatat tttttccatt atttacccta cttgaggatt
     2161 ttggatattt gccaagagtt gcatttaact tagaccacct atttaaaaaa gcttgtgcac
     2221 atggaaaaca gtgtttgact atgtgtatgg gatttggatg taatgcagct ggagttattg
     2281 gttgtaggat aattgactct ccaagggaaa gacttatagc tatacttaca aacaactttg
     2341 ttccttgtaa tggtcgtttt cctactttga tagctatatc tacaatattt tttagctcag
     2401 ttataaccaa ctctttcgta tcaagcgtag caactgcact ttgcataact ttactgataa
     2461 ttctaggtgt tataataaca ttgttggttt cttatactct ttctaagacg ctattaaaag
     2521 gagttccgtc tacatttaca ttagaacttc ctccttatag agttcctcaa ataggaagaa
     2581 ctttatatac ctctataata gatagaacaa tatttgtttt aggaagggct gtaatggtag
     2641 ccatacctgc tggtgtcata acttggatat ttgcaaatat atatatagga gatttaagca
     2701 tattatcaca tgttgcaaac tttttagacc ctttagctaa attaattggt cttgatggtt
     2761 ttatattact tgcttttatt cttggattcc cagcaaatga gatagttgta cctatacttt
     2821 taatggctta cctagcaact ggttctatga tagaattaga tagctttagt gctttaggtc
     2881 aagttcttag agaacatgga tggacttatt tgacagcttt aaatgttatg ctgttcagtt
     2941 tactgcattg gccttgtgca acaacacttt taacaataaa aaaagaaact ggtagtttaa
     3001 aatggactgc cttaggattc ttaatgccta ctatacttgc ttttgttgtt tgttttttaa
     3061 caactacagt ttacaattta tttatataat tagtattaac tgacattaga catatattag
     3121 agaaactatt taaaaattta tatactttat tataaagcat agagatatat tcaatgataa
     3181 gtaaaattac ttaacatttg aatatatctc tattttaatt cttatctgga tacttgaatt
     3241 gaaatttcag tcatataatt atcttcatta ctttcacaaa aaaagtcaag tatactctcc
     3301 tcatacgaat ttccaataat tttatattca ttttcatata tatatttttt caatttttcg
     3361 taagatttat atgaatcctc atattttcca atatgtttta tgcaagcata cattccttct
     3421 ggctttacat atagcttttt atcctttatt tttttatcta tcttcaaata aaaacttgat
     3481 atacttaaat agtccccatt ttttatattt tcattactta ctattactcc caactcatat
     3541 ccttgataca aatttttcga tttacaataa ttggtgaaat ctattaattc catcataaaa
     3601 tcactattac tcatattact caaatcgctt gatagaacta aatattcttc aaactttttg
     3661 gatatataaa tttcttcact attcatttta gaaattcctt caactgttat atttattcta
     3721 ttttgcatct tagagcttat ctttcttagt ttttttattt tttctcttat ctttttttct
     3781 tcttctttaa gcattttcac acaaagctgg ggagttctat tttgtatata ctcttttatt
     3841 tcctctaaac tcattcctat ttctcttaat gattgtaata tatcaagtgt atcatattga
     3901 taaactgagt aatatctata attattttta tctttatatt caggagaaaa aatacctatt
     3961 ttgtcataga aaattaatgt ctgcttagat ataccacaca atttagcaaa ttcacctgtt
     4021 gtaaaatact gtttttccaa gggttataca ctccttttct ttttgttgtc tatatagtta
     4081 ctatattctt tattatttac taaatcaagt agttattatt ttagaaaatc aatatggttt
     4141 tgattttaaa gcttaaatat cagttagatg tcagttataa atattttatc cattcctctc
     4201 ataaaaaaga tatcccctct taacttttgg atggaatatt atatttatgc ttgttagagt
     4261 gaaaattgta ataggtgcta caataagata tttaattaaa cataaagtta attattgtaa
     4321 taaaaaatct ttagataaat tagagttaaa gtatggaaat atagatagag aaaaagctgt
     4381 taaactagaa atattttatc aatatttaat agaacttgaa tatatagaaa tgagtttatt
     4441 aataagaaga ttagatactg caataacatc attagcatta gtatctatta taacaatagc
     4501 atcatattat ttaatcagaa aaaatgtgta attatgtaag ctatttataa taaaacactg
     4561 ataaaattaa taataaaaag gtaataggat ttctatacta ccttttggtt tttataatat
     4621 agtgtaattt tattatattt ttgtatgcta atataactag ataatcataa gataacacat
     4681 taaaagaaaa ttataaaaat ttatattccg tatagttagt ataaaacgta agtaatataa
     4741 atttatgtaa aacaacaaaa gatggattta cattccatat agttaatata aaacgtttta
     4801 atgaattaac agaagatgaa agttttatat gtatttacat tccatatagt taatataaaa
     4861 ccattgaaaa tagtgacttt tacgaaaaat ttgctaaaat ttacattcca tatagttaat
     4921 ataaaacact tcctattaaa tccacattag gaaattctcc gagatttaca ttccatatag
     4981 ttaatataaa accccaaaat aaacttagta ttttcaacac ttacacgtat acaactctcc
     5041 taaatttgca gtgaacgagc aatagtgcaa ttgataacgc ttatcatgca ctctcaacac
     5101 cttatatttc aactgttacg ccaaatttta tcacaaatat agctcactgc aaaatttcta
     5161 taattttatt atatcataaa tatattactt ttgaatattg ataccaattt ataccataat
     5221 aaaagcaaga aaaaacgtaa tctatctaca ttggatttaa attctataac ttaaagtttt
     5281 ttacataata tttatgcttg tttaatcaat ctaatatatc attcttgtat attgctttta
     5341 atagtctttt ttcttaaata aatatctatc tgaacaagca tagtttaatt aaaaactttt
     5401 gtattccctt aaaactgtat ctacctctta ttattttatt tatgatatat tatttttaat
     5461 ctaaaaatag atatctattt ttattatagt tatccctttt taaaatactt taatcatata
     5521 ctactcctaa tttaacatcc cttgaaatgc tattttctta tattgcaatt tataattgat
     5581 atatcttcaa tataataatt taatgtattt ttgattttaa agcttttcag tgatattatt
     5641 gataaattag aattaattat ctagctttaa aaggtcaaag atttctgttg ctgtctgcgg
     5701 ttttccaaat aaatatcctt gtatgtaatc tggctttatt tgtttaacta aatcatactc
     5761 ttcttgtgtt tctacacctt ctaaacatac ttttattcct acatcatgac aaattgcaac
     5821 aataaaatgt ataaatgtag catcaaactt actctttaat atatctttaa caaatactct
     5881 atcaattttc actatatcta ttggagaaaa ttttaaaact tctagagacg agtatccagt
     5941 accaaaatca tccatagcaa tataaattcc taaatcttgt aaagccttaa atttaaactg
     6001 taaaatactc atattttgta ctgtatgact ttctgtcaat tctaaaacaa gattttgata
     6061 aggaaaattt tcatcatcta taatttttac tatatcttca ataaatgtgt cttctaatat
     6121 ttgaacagct gaaacgttta cactcacttt aaaaaaagga tagtaatcaa tccatttcct
     6181 aaaagtacgt aatgccattc gaagcaccca agcccctaca atatttatca tatcattttc
     6241 ttctaatatt ggaataaatt ccaaaggaga aattgctttg catttgtcat tagtccacct
     6301 taaaagaact tctacaccta ttattttatg actttgtgta tctacttgtg gctggaatcg
     6361 aagactaaat cccctaaaat catcattaat tgatgccttt aaatcccgca tcatttctaa
     6421 tgaatacatt ttatttttta atatttcttg ggagaaaaaa acaagcctgt ttttaccatg
     6481 ctctttcgca tattgtaaag agtaactagc gcatttataa agttctttta ctgtatctcc
     6541 atgttttgga tatattacac aaccagcaga aattgttata tttaatccat attttctcca
     6601 tagttgaagg tgtataatca tattctgtat ttgattatat agagttagaa tttcattttc
     6661 ttcaacatta tcaacaagaa tacccatttc gtccccatca agtttataaa gttcagcatt
     6721 atctggtaaa atagattgta tagactgtgc tagtgttttt attatattat ctccaaaatc
     6781 cctatcatac atctcattaa catttttaaa atcgtcaata tttaataata ctatgcataa
     6841 tttttcaatc ataggatttg aaattttatc ttcaaataca ctcataaatt catgataatt
     6901 cagtaactgt gtcaatgaat caatcttatt ttgtttacct aattgcgtca ttattcctgc
     6961 aaaaatactt ggttctccaa attcatcacg cattagttgt cctctacatc ttaaccatat
     7021 atattctcca ctacgatttt tagccctaaa ttccactgta tgataatcca tctggttttt
     7081 acctatttca gtgtttgact tataaaaacg attccagtct tctggatgta caattttttt
     7141 ccagtaaata agcggatttt caacaatttc tcctggtaag tcaaataatt caacttgtga
     7201 tggtgaatat cgaaatacgc ctgttttcat attacaaatg tatatatatt catctgtacc
     7261 tctaatcaaa gcatcataaa gcaatgtaga attataatca aaattgtctc ttgaaaaatt
     7321 aaaaactcct tttccttctt tagtatttga tatttcatta aattcattaa tcttaattgg
     7381 tttatttaag attgttgagt ttgcaatatc tattgattga tgcatatgaa caactgtgcc
     7441 atcttgccat gtaattaaac tatcataatt ttcaaaaaca cgatttaact tattacattt
     7501 ttcataccac tttattaata ctcctttttt atcattcttt aatagttcta aaactttaca
     7561 aaaactacaa gtagaatttt gctcaggata taatacttgc cagcaaactt ttccttctgg
     7621 atctaaaata ttatattctt ctttcatttt cttgttcata aaaataattt cattagtatg
     7681 aatatttgta acatatatat taatctgtag ttgatttaat ataacttcaa aattacgttt
     7741 gttcatatac ttatctcctc catagttact gctctttata acaacactgt taaaattatt
     7801 ctttttagaa gatttactta taacttatac atacacacta aataccttaa tttaattaga
     7861 aagtttatta tttcatattt taaattataa ttgataaaaa aaaatacagc aatatagtat
     7921 taccaattta aaaaaaatta atattacata atacatctct ctcttatatt aaattttaac
     7981 ttggtactaa tcttgacaaa ttgtccacaa aaataaatta tatattttat tattgtttta
     8041 aatttttata aaaaatcagt attattgata ctattgaagt taaaaagcat taatttctaa
     8101 aaacatcata atttttctaa tagacttaat cacttttaat atatatagta aaacaaaaaa
     8161 cttatatttt ataaatatca taattttttt actgatattt tattatataa attttataga
     8221 attaattgtc aattttttat gaaaatggta agatatttat ggatggtatc ttgttataag
     8281 aactatactt taaatgtttt taattatttt gtcttatgta actcatatcc tcaggaatga
     8341 gattagaaat taagatgttg tatcacaaag tggaggtgat actattaaaa taggctttat
     8401 tggttctgga aaagttggaa catctttggc aaattatttt ttatcaaaag gttattgtgt
     8461 ttcaggcttt tatggaagga atcaattatc tttattagaa tcaataaatt taacaaaaac
     8521 aaagatttac agcaatttga gagatataat ttatgaaaac gatatattat ttattacaac
     8581 atcagatgat tctattgaaa ttatagacaa aaaactttcc aaatttaatt taaaacataa
     8641 atacatttgc cacacaagtg gctctttaaa atctagtata ttgttctgtt caaaaaaagc
     8701 aggtgcatta atttattcta tccatcctat atttgcattt tccaataaaa ataccaacat
     8761 aaaaaaaatg aaagacattt gtttttccat agaaggtgat aatgaacaag atgatttagt
     8821 tattcaaaat ttcatagatg gtttgggaaa ccaatttttt ataagagata aggatacttc
     8881 atccacttat catctagcta atgttttagt ttcaaattta gtattatctt tactagatat
     8941 aggagtaagt tactttatta aattaggttt atctgaagag gaatctttaa aagctatcag
     9001 tcctttagta aaaaaaaata tagaaaatat acttgataat ggctttacta aagctcttac
     9061 tggtcctgtt tctcgtggag atataacacc aataaggaaa catctatctg tattgaataa
     9121 aaaagatgag gatatctaca aaatactatc cttaaaccta ctaaaaataa ttgccttagg
     9181 aaatgacaat tcattaaaag gtacaaaaaa tattactata gaaaatgcaa tcgaaaattt
     9241 aataagtaaa tctgaaaaat acaaagaaat ttacaaactt ttaggaggaa aaaacgatga
     9301 aaaataccat acagactttt aagaacgcta aatatgaagg aaaaaaacta tctatgctta
     9361 ctgcttatga ctactcaatt gcaaaaataa tggatgaatg tgatataaat ggtattttaa
     9421 taggcgattc attaggaatg gtcataaaag gtgaagaaaa cactctatct gttactatag
     9481 acgaaattat atatcataca aaagctgtaa aaaatggtgc aaaaaatgcc ttaatcgtaa
     9541 gtgatatgcc ttttctatct taccatgttt ctatagaaga tgcagttaaa aacgctggta
     9601 gacttataaa agaaggagga gctcatgctg taaagttaga aggtggctca aacgttataa
     9661 agcaaataga aagcattgta aatgctcaga tacctgtaat ggggcattta ggattaactc
     9721 ctcaatcagt aaattcattt ggtgggttta aggttcaagg aaataccagt gaaacagcaa
     9781 gacaacttat tgaagatgct aaattaatag aaaaagcagg tgcattctct attgtccttg
     9841 aaggagttcc tacgaaaata gctgatatgg taactaattc tatttcaatt cctacaatag
     9901 gtataggggc aggaattaac tgcgatggac aaattttagt ttatcaagat atgttgggag
     9961 tgtttggaga ttttgttcct aagtttgtaa agcagtatgc aaatatagga gatataatga
    10021 aagattcaat taaaaattac atcctagaag ttaatacagg tgcatttcca caagaaaaac
    10081 acagcttttc aataaatgaa tctgagttag aaaaattata ctaatattaa ggatggtaca
    10141 aaatgcttgt aaaagaaatt aaattactaa gaaatataat caaagattgg agaaaacatg
    10201 gctattctat agggcttgtt acaacaatgg gatttttaca tgaaggacac caaagtctta
    10261 taaaaaaagc tgtaaaagaa aatgataaag tagtagtgag tgtatttgta aatccaactc
    10321 aatttggacc aaatgaagat tttaatagct atccaagaga tatagataaa gattttaaat
    10381 attgtatgga tagtggagca acagtagtct ttaacccatc accagaagaa atgtatttaa
    10441 aaggtaattg tactaccatt aatgtttctg gacttacaga ttttctttgt ggagctaaaa
    10501 gacctgttca ttttggaggt gtctgcctag ttgtttcaaa gtttctaaat atagtaacac
    10561 ctgataaagc ttattttgga gagaaggatg cccaacaatt ggctgtaatt aaaagaatgg
    10621 tcaaggattt aaacataaat acagaaataa taggctgtcc tataattcgt gaaaatgatg
    10681 gattagcaaa aagttcgaga aatacatatc tctctgagga agaacgaaaa tctgctctaa
    10741 tattaaataa gagcttaagt ttagcaaaag aagaattagt aaaaggcaat ctaaatccaa
    10801 agaatataaa agaattaata acagctaaaa taaattctga acacttagca aaaatagact
    10861 atgttgaaat tgtagatagt gagacattac aacctgttaa gcaaatagaa cattctatcc
    10921 ttgtggcaat tgctgtgttt ataggaaaaa ccagactaat tgataatttt acatttgagt
    10981 taaatatata gtatataatt ttatataata tatctaaaaa ataatagaat acctaaaaag
    11041 taataaatta cctaaaaaat aataaattgc ttagaaaata atagattaca ataactaaaa
    11101 ttttaaaatt aaattcctta aaagtatgat taattataat tatataagta tattcttatt
    11161 acacttatat taatttatat taaatatcta ctaactcaaa tattcataa
//
LOCUS       DAEPLF010000112        11129 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEPLF010000112 DAEPLF010000000
VERSION     DAEPLF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.112"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..1173
                     /locus_tag="KR314_002386"
     CDS             <1..1173
                     /locus_tag="KR314_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0328763.1"
                     /translation="NLEKIKKFFGFPLDKKHKENVEKELCNYLYHTGKIICIIIVISQ
                     IIMMISISAREGGPFATPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYINL
                     WIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLFL
                     NLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEISH
                     INNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNYG
                     HQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCDETDAKLKSECLRKIIE
                     SCNFERDDVPLKCVTVSIGVYTKKIEKAITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(1346..2743)
                     /gene="asnS"
                     /locus_tag="KR314_002387"
     CDS             complement(1346..2743)
                     /gene="asnS"
                     /locus_tag="KR314_002387"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBH0328764.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(2820..3053)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3222..3416)
                     /locus_tag="KR314_002388"
     CDS             complement(3222..3416)
                     /locus_tag="KR314_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBH0328765.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(3690..5363)
                     /locus_tag="KR314_002389"
     CDS             complement(3690..5363)
                     /locus_tag="KR314_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBH0328766.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPVDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSQLNIAINRND
                     GSLSQDNIGTGTLVSGILAGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     ISGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(5373..8771)
                     /locus_tag="KR314_002390"
     CDS             complement(5373..8771)
                     /locus_tag="KR314_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBH0328767.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNTLLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVVTVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIISFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSSINQDLETEDIGYRSINKSFKDEENRYKFIDGYNIQI
                     HNDLENEIYISKNASRQSGILSGIDVVHTPEFEEELAGLGMSQNFFKISDSLGVLSIN
                     NTDYSSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICSGLGNVNSEYAGIAEDSELIIIKLGKIDGFYNS
                     AMLFAASQYAYKKAFELRRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPHVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITVGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(8937..10133)
                     /locus_tag="KR314_002391"
     CDS             complement(8937..10133)
                     /locus_tag="KR314_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBH0328768.1"
                     /translation="MFIEFFYLLRSRGLNISLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMLSR"
     gene            complement(10154..10975)
                     /locus_tag="KR314_002392"
     CDS             complement(10154..10975)
                     /locus_tag="KR314_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBH0328769.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         3542 a         2046 c         1289 g         4252 t
ORIGIN      
        1 aacttagaaa aaataaaaaa attttttgga tttcctctag acaaaaaaca caaagaaaat
       61 gttgaaaaag aactctgcaa ttatctttat catacgggta aaatcatttg cataataata
      121 gtaatatctc aaataattat gatgatatca atctcagcta gagaaggagg cccttttgcc
      181 acaccaagac gccaatggta tttttatatg tatgtaacac tattttgtgt atcttctgtc
      241 tttatgtttg tattttctta tctttggaat aaaaaaaagg attgttgtac gttttatata
      301 aatttatgga taatttatgt gacttttttc tgcctatggg gttgtgtaat tactctactt
      361 gaccagttag gtggaaatga cctaagtgta tttagttata ttatgttagc agctgcagct
      421 ttctcagtat taaaatttgg agaatcagta atagtttttg gaggatgctt tttgtttctt
      481 aatttattct tacctgggat tcaaactata ccaggcaatg agtttagtaa tattgtcaac
      541 acatttttta taacatccct tgccattttt gtttctacaa tgctatatag aaatcgtgta
      601 tttacttact atgataaact tttaattagt caacaatatg aagaaatatc tcatataaat
      661 aatttactta acgacaaagt tatgacagac gaccttacta aaatgaacaa ccgtagatac
      721 cttgaagata ttctacaaaa aaaattaatc agccatgcaa atcaagaacc tataactgga
      781 atgatgatag atatagatta tttcaaacaa tttaatgata attatggaca tcaaaaggga
      841 gactattgtc tacaaaatat tgccaaagta attttagatt ttacaagttc tgaaaatgaa
      901 catgctatac gatatggtgg tgaggaattt tttatatgta tttttaactg tgatgagaca
      961 gatgctaaac ttaaatcaga atgtttacga aaaattatag agtcctgcaa ttttgagcgt
     1021 gatgatgttc ctttaaaatg tgtaactgtt agtattggag tttatacaaa gaaaattgaa
     1081 aaggcaataa cattaaatga gtttataagt aattgtgata aggctctata tactgcaaaa
     1141 gaacagggac gtaattgtgt tgcattttat taaaaagtta catactatta tataaaccta
     1201 taagtattat atcgcaaaat aaaagtaatc aaattatata tttatagtac aataaaaata
     1261 acttgcatat ctctaaaata gataaatatg caagtcattt tagtgtttat acttaaagtt
     1321 atataagttt tattatatat aattattaaa attcagcatt tttaggagtt cttgggaaag
     1381 gtataacgtc tcttatgtta gacatacctg tcatatacat aattattctt tcaaatccaa
     1441 gaccaaatcc agagtgagta gcagtaccaa actttctaag ttctaaatac caccaataat
     1501 cttcttctct taatcccatt tcattaattc tatctaacaa tacatcttct ctttcttctc
     1561 tttgagaacc tcctacgatt tctccaactc caggaactaa taaatccatt gctctaacag
     1621 ttttaccgtc ttcattcatt ctcatataga aagcttttat atcttttgga taatctgtaa
     1681 caaacactgg acaattatat atttgctccg ttatatatct ttcatgctca gtttgaaggt
     1741 cacatcccca ctcaacagga tactcaaatt catgaccaga ttcaattaat aattccactg
     1801 ctttagtata cgttattcta ataaactcag agtttactat cttatttaat ctttctaaca
     1861 atcctttatc tataaaacta ttaaaaaatt ccatttcttc aggagcattt tctaatacat
     1921 agcttataac atattttatc atatcttctg caagttccat gttatcttca aggtctgcaa
     1981 aaactatttc tggctctatc atccaaaatt ctgaagcatg tcttcctgta taagagtttt
     2041 cagctctaaa tgtaggtcca aaagtataaa cgtttctaaa agaaagtgcc attatctcgg
     2101 catttagttg accactaact gttaaattag cctcttttcc aaagaaatct tgagtatagt
     2161 ctattttacc atcttcagtt tttggaatat catttaaatc catagttgtt attctaaaca
     2221 tttctcctgc accttcacag tcactacccg ttatgattgg agaatgtgca tatacaaagt
     2281 ttctttcttg gaagaatttg tgtattgcat aggcagctat tgatcttact ctaaatacag
     2341 ctgaaaaagt attacttctt ggtcttaaat gtgctatcgt tcttaaatat tcaagcgtat
     2401 gtctcttctt ttgtagtgga tacgaactat ctgattcccc ttctacaact atgctttttg
     2461 catgtatttc gactggttgt ttagcccctt ctgtagatac taactctccc tctacaagta
     2521 tagatgagct tattgctaat tttcctacct ctttaaaatt ttctactttc tcatcaaata
     2581 ctatctgcat atttttaaag aagcttccat catttagctc tataaatcca aaatttttag
     2641 atactctaga tgttcttatc catccagcta cttttacagt cttccctatg tactgttctt
     2701 tttctctgta caattctttt actgttataa attccttttg cattttaaat cctcctagta
     2761 taattgttat taatttcttt taaatataat ttcaataaaa aaagcccttc atccctatat
     2821 aaagggacga aagactatta ctctcgcggt accaccctag ttgtcataat gacctcttag
     2881 ttaagcaatg cttaaagcct ataacggtgc cttccgtcta agtctactta ctaaataagc
     2941 tttcgattag aaactccaag atgttcttca atataaacct cgtattgagc tttcaccatc
     3001 ctcaactcgc tagaactaca ttttatatct actcttctct tcacagtatt tatcttttat
     3061 tatttctact aattaacaaa attataccaa tttataaatt tttagtcaat aaatttatat
     3121 aattgatact gattgttatt taaaatataa taattctacc ttgcataatg ataataaata
     3181 cttatcttca ttacttcttt attacttaaa tttccaaaat tttataattt acttccattg
     3241 tcaacccagt cttttgcttc ttcgacaccc ttaattgaag gtattgaaac tttactatga
     3301 ggttttaatt tttcaacatc agcccatgct gctgtcgact catttccaac taaaagtgaa
     3361 cttttaagtc ttttattatc tttacctttt aatgctcttg cctttttact ttccaaaact
     3421 atcacctcta ttatttattt tgtaatagtt ttagcaattt tacaaataaa attcataagt
     3481 ctaaattata atgattttta aattaacatc atatcttttc aatacttaat ttatacctat
     3541 ttatatacaa aatatttact ttgtgtattc aaaatataaa actgtttttt taattttcat
     3601 atttattttt tatgccattt ttctactcta tctaaagtat ttgatacaat taaattacta
     3661 atcaattata attttacata ggttcttatc tatagagtat ttgctatctg ttgaatcgta
     3721 ttttttaaat ttaaaatccc atatccctgc gatacatttg gatatgtata tatctcaagt
     3781 tttgtagcac ccaatattaa atatgttttt aaaacttgtg taaacaatga taatctcggt
     3841 acattatcct gtttttctaa gtattccatc aataaggcta aaacacctgt gactatagaa
     3901 ctgctcactc ctgttcctgt tcctgtatta tatgttccat ttttatatgt ggatattata
     3961 tctacacctg aagctactat atctggctta attcctcttc ctctaattgg tcctttcgaa
     4021 gaaccaatcc acatactatc agtcttatta ttaaatgtac caactgtaat tacatcatca
     4081 ctagcagcat acatagttat agtagctact gaatcagggt ctaaaaacct tgtgtctgga
     4141 gctatcaaat ttttatttgg taagtatatg tcatattctc cacttattat aagttctgaa
     4201 gtcaatctta aaatccatac tccaggtttt atatctctta atcttatctc caaattttct
     4261 tttccagagg taatatatgg ataaataaat ctcattgcat aagtagtatt ttctaagtta
     4321 aattttcctc tatatatata aaagtctgga gaatacctta tatcatgact aacttcacct
     4381 gaaggagata ttatctgtgc tcccacttta tctggaccat tcgtatttag tgttatatca
     4441 agagcatagt catctccatc ttgtattatt acatcttgaa cttctcccac actgctaaat
     4501 ctgccagaat aatgaatatc tgtattccct tgatttcctg caccactcac tactaccaca
     4561 ccagcactac tcaatatatt aaaagtatct aatattgaag tagttgctac agcacttgac
     4621 ttaactccaa tagtcaaatt tattattagt ggtttatttt ctgttcttgc tatattagta
     4681 acatacgtta tagctgctaa aaaatctgaa acactataat ttatccttcc agcataataa
     4741 gtatctgtat atgattttaa tttaactact attaagtctg attctgtagt aatacctcta
     4801 tactgcgaat ttatccttcc atttccagct aatataccag aaactaaagt tccagtacct
     4861 atattatctt gactcaaact tccatcattt ctatttatgg ctatatttaa ctgacttctt
     4921 gtaaattcac ttccaaaaat aaaaccttct ggaggtggat ttgtatttgc ttcttggtcc
     4981 cataaatata gaactttact agttccatca tcatttataa aatcaggatg caaataatct
     5041 ataccagaat ctataactgc taataaaatt cctcttcctg ttatatcatt atatggattt
     5101 tcataaatat aatcagtttc agctgctgtt gtaatggttt ctccattatt tacattatta
     5161 gttatttcaa ttagactgct cattggttca gattcttccc accaagcaac ttgtattata
     5221 ttatttaaaa tagtttcatc aaaatctaca ggtacatata ttactgcaag ctgactattt
     5281 aaaaccatat acctatttat tccattttct tgtagggcag attctatatc accttgataa
     5341 attatacaat aagatttttc cataaatccc tcctatctta aaacatcaaa cattcctcta
     5401 acatccaaaa gcccatatcc agaatttgta tttggatata ctgtgtttga atcttttcta
     5461 gcaccagctt gcataaaagt ttttattttt tgcacatatg cttggtttgg atatctacca
     5521 tcaacaaaag tatattggaa atacattgca gcagcaccag ctgcatgtgc acttgctgct
     5581 gctgtaccag ttatagttgc atacgtgttc ccaggataag cagctattat attgacacct
     5641 ggtgctacta tatctggttt taacctgtct tcaatagttg gtcctcttga tgaactttgc
     5701 cataaactac cattaatagt attgtaggct cctacagtta ttaaatcatc ttgaatagca
     5761 ggataattta tagtgtaaaa agggtctacc tctctaaatc tagttccact ttttaaaagt
     5821 tctctatttg gaaggtataa gttatatctt cctgttataa tataaacccc tactaatcga
     5881 atcttccata cacctctttt tgcattcttt aaagtaacat tagtaaattg ctgcccagaa
     5941 aaagttgttg ggtatatata cgttatagaa tattcagtcc cctctaaatc aaataaacct
     6001 gttactttat tataatttga aattccaaca cttttacttt cttcaccagt gggagatact
     6061 attattacat cagctttatc tggtctattt aaccaaagtt ctaatgataa ttcttcctcg
     6121 tcttcattta actctaattc aacttcaaca gaacctccaa catgtggtat tattccagat
     6181 gtatgtgtct gtgtattgcc ctcatttcct gctcctgctg ttatacataa ccctctagta
     6241 aagaatgctt tttcactatt gctcctattg gttaaacctg ccaagctact ggtacctaaa
     6301 gacatattta taactagtgg tcttctaagt tcaaaagctt ttttatatgc atactgagat
     6361 gcagcaaaca acattgcact attataaaaa ccatctatct taccgagttt tattattatt
     6421 agctcagaat cttctgctat acctgcatat tcactattta catttccaag ccctgagcat
     6481 ataccactca acatagttcc ttgaccaact tcatcctgag atagactagg gtcattttct
     6541 gcgatagctc gatttatatc ttctcgtgta tattctgtcc caatataaaa accatcaggt
     6601 ggagttcctt cttttgtttg gtcccataaa tatactattt ttgaagtacc atctggataa
     6661 ataaaatctg gatgtagata gtctattcct gtatctgcaa ttgatattag agtccctctt
     6721 cctgttatat taatattagg attattttta aagaaattta cgcccatttc ttctgttgca
     6781 actacacctc caaaagtacc tcgattaatt tctccaagta atgtcatctt tgtagtcgat
     6841 acagttctta tgattgaagg aagttgcaaa actcgttgta tactactata gtctgtatta
     6901 tttatagata gtacacctaa tgaatcactt atcttaaaaa aattttgact catacctaac
     6961 ccagctagtt cttcttcaaa ttcgggagta tggactacat ctattcctga tagtattcct
     7021 gattgtctac ttgcattttt acttatataa atttcattct ctaagtcatt atgtatttgt
     7081 atattataac catctataaa cttataccta ttttcctcat ctttaaatga cttattaata
     7141 cttctatatc caatatcctc cgtttctaaa tcttgattta tactagatag agtgtacaaa
     7201 tctatatctg ataaatttaa aaaaccaaat cctgatgaat tatttggata agattgatta
     7261 cttaatctcc ttgctccctt taaaagtaat gcccttaatt tctgtgaata caaaaataaa
     7321 tcatttccat ttacaattcc ccattccata aacaatgaac aaacccctgt aacatgaggt
     7381 gtagccatgc tagttccagt taatgctcca cttgtacctc ctggtaaaaa agatattata
     7441 tcttcacctg gagctaataa atcaggttta aaaacaccta attgtgtatc tccttcacca
     7501 gaaaatatag atactatgtc tgttcttgaa ttaaaactac ctactgttac aactctactt
     7561 gctgttccag gaactgttac agtaagctct tgagtaggaa tcagaaatct agtatttcta
     7621 tttagtcctt cagaagttgg caaatatata tttacattac cagttactat atctattggt
     7681 tcaaatacaa ttttccataa cccaggagtt atctgtgtat ttgaacttaa ttgtagagta
     7741 actcttcttg taagagagta cggtgctata gggtaaaaat aaccagttat tctagtttca
     7801 cccaaagtat ttcttatctc acctgaagtt agagagattg cttgtgtttg attgtttgat
     7861 gggtttacca aatgtacact aaaatcatca acaaaatctg gccatatatt tatgtttaat
     7921 atacgctctc cttccccaac tataaactca acttcttctg taatattatt ttgaagccta
     7981 atccttttat gacctccttt atctgcatta tttccagctg ctacaactat attgtttttc
     8041 caaaataaac acatatcatc tatatattgt tcaaataaag atgttcctct atgtgaacct
     8101 tcattacttc cataacttat atttaatgta acaggcattc ttaattctaa tgccctatct
     8161 aaaataaatt taattgccct cataaactct gtacttctcg aaaaaatatc ggtctgtata
     8221 tttccaactc taaccactat tattctggca tcacttgcaa tagttgcaca aatacctgca
     8281 acatgtgtcc catgtaaact agttgttgaa ataggtatat acatacttcc atctattgca
     8341 ttatttatat cttcattagt atataatgtt ccttctctaa aaccttctgg tggatttcct
     8401 tgaatagatt ggtcccaata atataatatc ttagacctcc catcactatc tctaaacaca
     8461 ggtagagtat aatctattcc cgaatcaatt ataccaatta tagttccctt tccagttaaa
     8521 ccagttctat ttttaaaccc cgttattcct gtacttgaaa aactttgcac atcctgagtc
     8581 tgtaatataa aaggcttttc tataaattca atttctggat aagttaaaag tgtatttaca
     8641 tcttcttcat tacttgatgt tattattgca tatgatgaat taagtatctc tactgataca
     8701 cctaattctt cttctaatct caatatatct ccattgtatt ttacaattaa ttcataattt
     8761 attataatca cctcgacttc taaaattatt attaatgtat atgttttgtg taagtctaaa
     8821 atgtattaga ctatataatt ttttaagttt cagttataca atttataata gatatattaa
     8881 aaaataaata atgatggtac aattttgaat aaatcatacc atcatttata tttactttat
     8941 cttgaaagca ttaaattttt caatgctttt tcaagacctt ttacagtcag tggaaacata
     9001 ttaaattcac tttgtatcat tctttgagtt tggtaccaat aattaattga atcccatcca
     9061 tctctaagtt ctggattcat ccatacaatt ttactgtact ttctcttaaa ccttttaagc
     9121 cattctatac caggtgtgta attatatgga ccacgatagt taccaccaat atgaagtaac
     9181 tcagaaggcg acatagaagc atctccaaca attataactt tatagttttt atctatattt
     9241 tttaatatcc attcagtatt aatagattta cttaaccaac attcaggtgt tgtaaataat
     9301 ctatcataaa tacagttatg gaaataatat atctttacat ctttaaaatg atttgattta
     9361 cttacagact gaaataatgt attacataaa gtgctatacc ccctcattga accacctgaa
     9421 tccataagca ataaaagctt cactgtattt tttcttggct tctcaaaaaa taactttaaa
     9481 tatcctgcat tattacaagt ctcttcaata gtcttatcca aatcaagctc tgtctttgga
     9541 gcatctattc tagtagaaaa ttgacgaagt ctctttagtg caacttgaaa ttgacgcata
     9601 tttaaaactt catcatctct aaaatctctg tattttcttt ctcctgcaac ttcaagaaca
     9661 gctctaccat aagtagaatt tccacctata cgtataccag tttgcccttt tcctgaatgt
     9721 cctaactcgg aagtgcctcc tgtgcctatc caataattac caccattatg aggagagtct
     9781 tgttctttta ttcgttcctc caatttagct cttaatttat caagatttat atttggattt
     9841 tgttccaatc tttcaaactc ttctaaatct atatctggtt ttttaagcca attcataatc
     9901 tcttctggaa tttcttcttg gtgttctata cctttaaaat actccaaaaa cactgcatca
     9961 aacttatcaa aatcactttc actttttatt aaaatcatcc tgcaaagata gtaaaaattt
    10021 gaaaagtttg aatagcacag ccctttatcc aatgcttcaa ttaaagtcat ccattcattt
    10081 aaagatatat ttaatcctct tgacctcaat aaataaaaaa attcaataaa cataattatc
    10141 cagctcctat taactataag tgtctttgca tagattcaat atcctcatta ttcttaagta
    10201 gtattcctgc aaaaggaact tctttttcta ttttttcaat aggcattcca ctcaaagtta
    10261 atgcttgtat ccagtctatt aactctgatg tactaggctt tttttgtatg tcctttaaac
    10321 ttctaatcca ataaaaagta gtcattactt gttccaatag gtgttcttct actttatcaa
    10381 aatgtacttt aactatttct tccatcatgt ctctatcagg aaattctata taatgaaata
    10441 tacatcttct taaaaaagca tctggaagtt ctttttccgc atttgaagtt ataataacta
    10501 ttggtctttg tttagctctt acagtttctt ttgtctcatt tatgtaaaac tccattttat
    10561 ccaattccca aagcaagtca tttggaaatt ctaaatccgc cttatctatt tcatctatta
    10621 aaagaattac ttgttgattt gagctaaagg cctctcctag tttaccatat tttatgtact
    10681 tgctaatatc atctacacct tctcctccaa attggctatc ataaagtctt tgaacagtat
    10741 catatacata aagcccttct tgtgcttttg tagttgattt tatattccaa attactaaat
    10801 ctttttttaa ttcatttgaa attgcttgtg ctagcatagt tttacctgtt ccaggttcgc
    10861 ccttaatcaa aagtggcttt tcaagagcaa tagcaacatt aactgatgcc atcaattctg
    10921 gtgatactac ataatcacta cttcctctaa agttagatat ctcttttttt atcataaatc
    10981 ctactccttt taaacagtat tttctaaatg tatatagact ttattactat ttttgatata
    11041 aaaataataa tacaatacat ttgtatagta ttattctaaa attctatatc taaataaaat
    11101 cacctctaaa atatttaatt tttatcttt
//
LOCUS       DAEPLF010000113        10966 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEPLF010000113 DAEPLF010000000
VERSION     DAEPLF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10966
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.113"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..496
                     /locus_tag="KR314_002393"
     CDS             <1..496
                     /locus_tag="KR314_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0328770.1"
                     /translation="SLNKLAKLQRELSRKTIGSLNRNKARLKVAGLQEHIANQRNDFL
                     QKLSTKLIKENDIICIEDLQVKNMIKNRKLSRLISDVSWSEFVRQLEYKANWHGRQIV
                     KVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASINILKEGLRLIT
                     IQNK"
     gene            639..1187
                     /locus_tag="KR314_002394"
     CDS             639..1187
                     /locus_tag="KR314_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0328771.1"
                     /translation="MGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKADIEPVL
                     LKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEERLSEFI
                     NGNTLKYDFVKMPHHGRYDKLTETFLESTSPQYAVITCSEKKEPEEGVLKILERLNIK
                     TFLTSSGEVVINSDGKTLSVNQ"
     gene            1306..1497
                     /locus_tag="KR314_002395"
     CDS             1306..1497
                     /locus_tag="KR314_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328772.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            1720..2070
                     /locus_tag="KR314_002396"
     CDS             1720..2070
                     /locus_tag="KR314_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328773.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKTVTSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            2332..3837
                     /locus_tag="KR314_002397"
     CDS             2332..3837
                     /locus_tag="KR314_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBH0328774.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            4227..5324
                     /gene="dinB"
                     /locus_tag="KR314_002398"
     CDS             4227..5324
                     /gene="dinB"
                     /locus_tag="KR314_002398"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBH0328775.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGKPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKSSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            5580..6716
                     /locus_tag="KR314_002399"
     CDS             5580..6716
                     /locus_tag="KR314_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328776.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            6709..8256
                     /locus_tag="KR314_002400"
     CDS             6709..8256
                     /locus_tag="KR314_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBH0328777.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            8590..9456
                     /locus_tag="KR314_002401"
     CDS             8590..9456
                     /locus_tag="KR314_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH0328778.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            9597..10739
                     /locus_tag="KR314_002402"
     CDS             9597..10739
                     /locus_tag="KR314_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBH0328779.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
BASE COUNT         4261 a         1153 c         1949 g         3603 t
ORIGIN      
        1 gtcattaaac aaacttgcta aattacaaag agaattatcg agaaagacaa tcggtagttt
       61 aaataggaat aaagcaagat taaaagttgc aggacttcaa gaacatatag ccaatcaaag
      121 aaatgatttt ctacaaaaac tatctactaa attaataaaa gagaatgata ttatttgcat
      181 agaagattta caagtaaaga atatgattaa aaatcgtaaa ctatctcgct taatttctga
      241 tgtgtcatgg tcagaatttg ttcgtcaatt agaatacaag gctaattggc atggtagaca
      301 aattgtaaaa gtaggcaaat tctttgcaag ttcacaaata tgcaataaat gtgggtataa
      361 aaatgaggaa gttaagaact taaatataag agaatggatt tgtcctagtt gtaatgaaac
      421 tcacgataga gatataaatg caagtataaa tatattaaaa gaaggattaa gactaataac
      481 aattcaaaat aaataacgta tgtaagaacc gtaggaacta cggggatagc ctgtggagaa
      541 ttagtaagac atatgttagt atgcaaaatt ctatgaagca ggaaccctgt gactttagtc
      601 ataggaggtt cagtttaacc catttagata aggaccatat aggtggagta gatagtgtgt
      661 tatctagtgt taaagtaaag aatattatac aagcaaatta taagaaagat agtaagcaat
      721 atgatgaata tattgactca cttaaaaaag ctgatataga accagtgcta ctaaaagata
      781 atatgaatat tgtaatcaat agtgcagaaa taaacataca tccggcttca aaaagcaagt
      841 acgagagttc aaatgattat tcaataatca ctaatataag ctatggagca cataagtttt
      901 tatttgcagg agatgcagag gaagaaagat tgtcagagtt tataaatgga aatactttaa
      961 aatatgattt tgttaaaatg cctcatcatg gaagatatga caaattaact gaaacatttt
     1021 tagagtctac ttcaccccaa tatgctgtta ttacatgttc agagaaaaaa gaaccagagg
     1081 aaggtgtttt aaaaatattg gaaagattga atataaaaac atttttgact tctagtggag
     1141 aggtagttat caatagtgat ggtaagacat tgagtgtgaa tcaataattt tttttaaaat
     1201 atctaaaaaa aatttaaaaa aatcattagc aaatattacc ccttaaacat agtatatttt
     1261 aggagctgaa actccacata caaaatgact aggaggtaac atattatgtt ggatagattt
     1321 tttggtgaag gcggatttgg tggatgtgct tggtggataa taatattatt cttcttattc
     1381 ttagcattcc aagattgttg ggctgaaatg gacttatgtg catggatacc tttcttaata
     1441 ttattattaa tactttgtga atgtggtgac gtttttgatg gaggatgtgg atgctaaaaa
     1501 aatcaattta gatgatttaa taaagcttat ggtgggtata aataaaatac caaaccaatc
     1561 acaatctagt gtttgaaggt agtcggaaat cccccgctac cttctttttt ttattgttag
     1621 aatttatttt tgaattattt catttacata taataatttt tacaataaag tctgatataa
     1681 ttaaaaagaa aataaaattt atttatagga gatgtaaaaa tggctatagt ggataagatt
     1741 ttcaatgaga taaaacttat ggttgcaaga cttttattgg gaaaaaaata cagtgaatat
     1801 gaagaaaaag atttagtgta taatacagaa gaagaagtta tacttataac tttaaaaaga
     1861 ttagtttttc agggaaatgt aaacgaagca gaagaaattt tatttgataa agctaagact
     1921 gtaactagtg aaaatatgca gtatatagca atagaatttt atactatgct tatggaaaag
     1981 actgatgaag aattagaagc tatgaatttt tctaaacaag aagtttatca aggtatagaa
     2041 gatattagaa aagtattgaa tttagaataa aagatatatg aaaatattga aataaattaa
     2101 tgttaaggtg agaatattat acttatatat aaaaatataa tattcttatt ttattagtta
     2161 tattaggtat atcttattta ccataaaact atcttgaaaa aaattaatct taattgtata
     2221 atattacttt gagtgaaaaa gtattaacat ttttttataa gatgttttac taaaaaaata
     2281 tatataaata tactgttatc aacaagaaat ttaatttagt gaggtgttaa gatgaaagta
     2341 ggatttgacc acaaaaaata tttagaagag cagtctaaat acatcttaga aagagtaaat
     2401 aattttgaca aactttattt agagtttgga ggaaaattga tgggagacct tcatgcaaaa
     2461 agagtacttc caggatttga tgaaaatgca aagataaaag tattacaaca tatgaaagaa
     2521 aaagttgaag ttgtaatttg tgtctatgca ggagatatag aaagaaacaa aataagagga
     2581 gactttggta tcacttatga tatggaagtt ttaagattaa tagatgattt aagaggttat
     2641 gaactagatg taaatagtgt agtaattact agatttgaag gacaacctgc tacaactgta
     2701 tttataaata aattagaacg tagaggaatt aaagtttata agcattatcc aacaaaggga
     2761 tatccgtcag atgttgagac tatagttagt gatgagggat atggagcaaa tgaatatata
     2821 gaaactacaa agccaatagt agtagtcaca gcaccaggac ctaatagtgg aaaacttggt
     2881 acttgtctaa gtcagttgta tcatgaaaat aaaagaggta atgtagttgg atattctaag
     2941 tttgagacat tcccagtttg gaatgtacct gtaaaacatc ctttgaatat agcttatgaa
     3001 gcagctacag ttgatttaaa agatgtgaat atgatagatt cattccacct tgaaacgtat
     3061 ggtgaaatgt cagtaaacta caatagagat ttggaattat ttccagtgct taaaaaaata
     3121 atagaaaaga taactggaaa agaatcaatt ttccaatctc caactgatat gggtgttaat
     3181 agagttggct ttggaataat agatgatgaa gtaataagaa aagcttctat agaagaaata
     3241 ataagaagat actttaaaac tgcctgtgag tataaaaagg gacaagttga taaaggcgct
     3301 tatgatagaa tgaaaatgat tatggaagag cttaacttaa agccagaaga tagaaatgtt
     3361 gtattacctg ctagaaatta cagtaataaa ttaaaagaaa gtgccgacaa gaatgatact
     3421 tgtcctgtag ttgcattaga attagaagat ggtacaatat taactggtaa gggttctgat
     3481 ttaatgaatg gaacagcagc agtagtatta aatgccatta aacatttagc caatatatct
     3541 gatgatatgc acttaatatc acctgtaata ttagaaccta tcataaactt aaaaacaaag
     3601 actttagcta gtagaaatgt ttcacttagt tgccaagagg tgttaactgc acttagtatt
     3661 tgtgctgtaa ctaatccaac tgcacagttt gctatggaaa agttagcatt attaaaaggg
     3721 tgtcaagctc attctactac tatattaaat agagatgatg aacaaacatt tagaaagctt
     3781 ggtatagatg taacttgtga cccagaatat ccttcacaaa atctttacta tagttaaaat
     3841 tatataaaaa aatatgggct gttttaaagt aatagtttga gcagtccatt acaattataa
     3901 taaaatcagt aggctgtctt gtataagaca gtcttttgtt tttaaaacat cccataaatt
     3961 aaatatatat actgttatcc ataaggaatt ttaaatttag ttccttggaa tacctattgt
     4021 aaagctttta tatagttttt gcaagtgttt ttaaggattt tttataaatt atattgaagt
     4081 taatggatta tagatggttt tatttatgga aaatatgaat atataaagaa aataatgaaa
     4141 aaataccaaa aagaaacata agtttggtat aattaaaaag taatataaat aaaattaatc
     4201 tatttatact ttaaaaggtg ataataatga aacattatag aaaaataatt catatagata
     4261 tggacgcttt ttatgcatct atagaacaaa gagataataa aaaattaaaa ggaaaacctg
     4321 ttattgtcgg tggaaatcca caaagccgag gtgtggttgc aacgtgttct tatgaagcta
     4381 gaaaatttgg tatccattca gctatgccat cagctgtagc ttacaatagg tgtccatatg
     4441 cagtttttgt aagacctaga tttgatgttt ataagagtgt gtcagagcaa ataagagata
     4501 tattttatag atacacggat ttagttgaac cattatcttt agatgaagct tacttagatg
     4561 taacaaaaaa taaaaaaaat attgattcat caattgagat agcaaagcaa ataaaaaaag
     4621 atatatttag agaagttggt cttacttctt cagctggtgt gtcatacaat aagtttcttg
     4681 ctaagattgc atcagactta agaaagccaa atgggttaac tgtaataacc gaagaaaatg
     4741 cccaggactt tttagataaa ttacctgtga ataaattttt tggtgttgga aaagttactt
     4801 caaatactct taaaaattta ggtataaaaa ctggatatga tttaaggtgt ttaaatttat
     4861 ttgaacttga aaatatattc aaaaaaagag gttatgaatt atataaattt gctagaggaa
     4921 tagacgatag accagtggaa ccaaataggg ttagaaaatc tgttggggct gagactacat
     4981 taagtcataa tttggatatt gatgaagaag aaacacgcaa cattcttgat gaactttgtg
     5041 aagaagtatg tcatagatta aaaagctctg agaagtttgg aaaaacatta acattaaaaa
     5101 ttaaatatga ggactttaca aagataacta gaagccttag tttagaacac tatattgacg
     5161 aatataatga tataagaagt ggtgtagata atttgttgag aaatgttgag gttaatggaa
     5221 aacaaattag gctattggga gttacaatat caaacttaag tgataaaaaa gaaacttata
     5281 aagatattac attatttgaa tacatggata gtattcaaat gtgagttttg tttaaatgaa
     5341 ataacactag ctttaatact atataattga ggctagtgtt attttatgaa tatttttata
     5401 ctttttgttt tttgacatta tatactatat ataaatactt tatatttgta gcaatattca
     5461 gacaaatagt gacctaaaaa tcgtactttg tttacataaa taaaatagta tgatataatt
     5521 ataaaaacaa cttataatat tggagaaggt tgcttgttcg aaaaagggga tgataaatta
     5581 tgatagagat aagaaatgta acaaaaaaaa taggaaataa tgttatatta gatgatattt
     5641 ctctagttgt agaaacaggt acattggtag ttttgattgg ttcaagtggt tgtggaaaga
     5701 ctactacttt gaagcttata aacaaattaa taaaacctac atctggtgag atatatatta
     5761 atggcaaacc aatttctcag gaaaatgaaa tagagcttag aagaaaaata ggatatgtta
     5821 tacagaacac aggcctattc cctcatctta caataaaaga aaatatagag ctaattccac
     5881 gcctaaaaaa ggaaaaatcc gttgaagaaa ttgaaaaaag aacgttgcaa ctacttgaga
     5941 tggtaggact tgatagtgat gaatttttga ataagtatcc ttcagaactt agtggtggac
     6001 agcaacaaag aattggtgtt gcaagagcca ttgctactga tgctgaaata atattgatgg
     6061 atgagccttt tagtgcttta gacccaataa cgagaacatc tttacaagaa caattatttt
     6121 ctcttcaaga tgaattgaaa aaaactataa tatttgtaac gcatgatatg gatgaggctt
     6181 taaagatagc tgataaaatt tgtattatga aggatggaag aatagcacaa tatgatactc
     6241 ccgaaaatat acttagaaag cctgcaaatg actttgtaaa agattttatc ggagaagata
     6301 gagtttggga taatccagaa tatataaaag caagagatat tatgataaaa aatcctattg
     6361 ctgttaattc cactagaact gtaactcaag gaatagaaat tatgagaacg agtaaggttg
     6421 atagcttact aattatagat agagcaaaaa cattaaaagg tattgttact gtaaaagata
     6481 tgaaagatat tgatgataag tcaatattac ttgctgatat tatgtcgagt gagcctttgc
     6541 atgtaaatga aggtgataat ttagtagaaa tactaaatgt aatgaatcgt aattcggttg
     6601 gatatattcc ggttatttct gatgaaaata aactggtagg gcttattact agaagtagtt
     6661 tactttctgt tttaagtgaa caattcttag aaatggaggt gagtgttctt ggctaatttt
     6721 tttaacttta tactattaca aaaagataag attatagagc ttttaataca acatatgagt
     6781 ctaactgtta catctatttt aatagctata atagttggtg ttcctcttgg aataattatt
     6841 tcaagaatct catctcttag aaagtttgta cttggatttg taaacttagt ccaagcagtt
     6901 ccgtctatgg cacttttggg tcttcttgta cctatacttg gcataggaag taaaccagcc
     6961 atatttatgg tagtggttta ttcattacta ccaattgtaa aaaatacgta tataggtata
     7021 acaagtattg acccagtagt attagaatca gctaaaggta ttggtcttac aagaaatcag
     7081 acattattta aaatacaatt ccctttagca cttcctataa taatgggtgg agttagaata
     7141 tctgcagtta cagctgttgg tttgatgact cttgcagcat ttataggtgc tggtggttta
     7201 ggatatttag tattttcagg ggttcaaact gtaaataata atatgatttt agcaggggca
     7261 atcccatcat gtataattgc actgttagtt gattatttat ttagtaaaat tgaagttgca
     7321 gttactccaa aaggattaaa tccaaaagca cctaagaaaa attatatagc acttaaagta
     7381 atatctgtag tggttgttgt ttcaatgttg ttcgttgttt ttagttctag tttttcaagt
     7441 aaaaaagata caataactat tggttctaaa gattatacag aacaacttat acttggtaat
     7501 gtttatgctg aattagtaga aaaaaatacg aatcttaaag ttaagaagaa tttaaatcta
     7561 ggtggttcat ctgttgcgtt taatgcaatt aaatctggtg agttagacat gtatgttgac
     7621 tatacaggta cacttttggt aaatgtaatg aaacatgctc cgattaaaga tgctgatgaa
     7681 gcatataatg ttgtaaaaga tactatggaa aaagaaaatc agttaacttt acttgaccct
     7741 cttggattta ataatactta cacattagct atgatgcctg aaactgctga aaagtatgga
     7801 ataaatacta tttctgattt gactaaatat ggtaaagaat ttacattctc accaactttg
     7861 gagtttgaaa atagagaaga tggtcttgta gggctttcta gagattatgg tttaaaattc
     7921 aaagatgtaa aagcaatgaa tggaagttta agatatacag ctttagataa taatgaatct
     7981 caggttatag atgcattttt aactgatggt cttattaaaa agtttaatct taagatatta
     8041 gaggacgata aaaatttctt tgtaccatat tatgcagcac cattagtaag agaagatact
     8101 ttgaagaaat atcctgagtt agaaaaagtt atgaatatgc tttcaggaaa aataaatgaa
     8161 gaaactatga gagaacttaa ctatcaagtg gatgaacttg gtaaatcacc agaagaagtt
     8221 gctcattcat tcttagttaa agaaggctta gtataaaaaa ttaatagttt atatcaaaaa
     8281 tttaagggag tgtctcaaaa tgaacttttt agttcatgag gcactctttt ttatatgaaa
     8341 tcaaatatat tttatcattt caacaataaa aaagaagctt atctttattt tgagacagtc
     8401 tcttattttt atgtatacca aaaccatacc tacgattttt atttataatt aagtttcaaa
     8461 tagaatgaac aagtacaacc aattttgaat ataaatatag taaatgtgct tatatacgat
     8521 tttattatct gggtgattga tattgataga tagctttctt tattgaatcg aagggagggg
     8581 gaatttgtta tggataacta taagaaagtg aagcaagtat tgtggataat tttatttgct
     8641 aattttgcag tagcattatt aaaaatcatt attggtaatc aaataaaaag ctatagtatg
     8701 acagcagacg gatttcattc tttgtcagat ggagcatcta atatagtggg gcttataggt
     8761 attttctttg catctaaacc aaaagataaa aatcatcctt atggacataa aaagtttgaa
     8821 attataacca gtttatttat aagtggtatg ttgtttgtta tagcaattaa gataattctt
     8881 agtgcagtgc taagaatagc taatccagtt gtacctgcta taactataga aagtttaatt
     8941 gctttaatta ttacactttt tataaatatt tttgtatgca tgtatgaata tagaattggg
     9001 actaaactaa atagttatgt tttaatttcg gattcattgc atacaagaag tgatatattt
     9061 gtgtctctag gtgtattggt tactttagtt ggtgttaagt taggatttcc agtaataata
     9121 gaatctatag ttcctattat tatttctgca ttcataattt attcggctta tggaatattt
     9181 aggccatcta ttggaatttt ggtcgataga gtggctgtag atgaggatta tataaaagaa
     9241 attgtgtttg aatttaatga ggtaagggat gttcacaata tcagaagtag aggtagcaaa
     9301 agttctattt atattgacat gcatgtaatg gtagacccat ttataagcgt agagcaatct
     9361 catgatttaa cacataaaat tgagaaacaa atacaagaag aaattaatga aaatgctcaa
     9421 gtaatagtcc atattgaacc tttttatagt ttttaactta gctataaaat acaagttata
     9481 aaaaaatata atttataaaa aaaatattat accataaata atttataaaa attatttatg
     9541 gtataatatt ttctataaag aaattaagtc ttaatatatc taaggaggaa tttacaatga
     9601 gatacgttga atatggaaag acagggaaaa tggtttcagt agttggctat ggaggattaa
     9661 ggtttgattt agaaaaaagt aatcaagaaa atgctgattt agtcaaatat gcgtatgaaa
     9721 aaggtattaa ttactttgat acagcgccag ggtattgtga tgacagaagt gaagatatat
     9781 ttggactagc ctttagacaa atgataaagg aaggtaagac cgatttttat gtatcaacta
     9841 aagaaaagcc aaaagcatgt gatacatcag aaaaagctat agaggctgta aaaaaatcaa
     9901 tagaacgatt aggcgtatct aaaataaatt tttatcatgt ttggaatatt agaaagatgg
     9961 aacattacga actagcaatg agacctggag gtcaatatga aggattattg aaatgtaaag
    10021 aagaaggatt gatagaccat atagtatttt catctcatca accaggagat gaggttatag
    10081 atattctaag tgaaaataag tttgaaggtg taactatggg aataaatata ttaaactttc
    10141 catataggct aaaaggtgcc aaatatgctg ttgataatgg atatggtgtt gtggcaatga
    10201 atccattaag tggtggaact attccaaagt ataataaaga actttctttt ctatcaaaag
    10261 atggcgaaag tactatagaa tcagctttaa gattcaacat aggaatttca caaataagta
    10321 tatctttgat agggtttaat aaaaaacaag atatagatga tgcatgtaaa attgcagacg
    10381 aaaatagaat ttattcagat gaggatatac atgatattga gactagatta aataagaata
    10441 tgaacgaaat atgtacaggt tgtggttatt gtaaagtatg tccaaaaggt attaataccc
    10501 cagcctatat gcttttttat aatgaaaagc agatgtttaa aaagagtgat gaagaaatga
    10561 caaagttagt ttatggttta ggacattgga attacacaat gaatagtaag gcaaaagcta
    10621 aagaatgtat atcttgtgga aaatgtgaag ttgaatgtac tcagcattta cctataatag
    10681 atagattaaa agaaattaaa aaatgggaag aagatggagc taatagtgtt aaagtctaat
    10741 taattttata ctatatttga aatttgtata gtatttagta atgttttaaa atatagtttt
    10801 aatatttaag gagacagatt ataattttgt ctccttaaat tatgctttaa tttgttatag
    10861 tttaattcca taagcaacat tgtatatttt tatataaagt ttttcaaata ttttacatta
    10921 atattgctta caaattctca ttttatacaa cactaatcta tgttat
//
LOCUS       DAEPLF010000114        10888 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEPLF010000114 DAEPLF010000000
VERSION     DAEPLF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10888
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.114"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            265..729
                     /locus_tag="KR314_002403"
     CDS             265..729
                     /locus_tag="KR314_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBH0328780.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            736..1230
                     /locus_tag="KR314_002404"
     CDS             736..1230
                     /locus_tag="KR314_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBH0328781.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1246..2271
                     /locus_tag="KR314_002405"
     CDS             1246..2271
                     /locus_tag="KR314_002405"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBH0328782.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2258..4705
                     /locus_tag="KR314_002406"
     CDS             2258..4705
                     /locus_tag="KR314_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBH0328783.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4885..6258
                     /gene="radA"
                     /locus_tag="KR314_002407"
     CDS             4885..6258
                     /gene="radA"
                     /locus_tag="KR314_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBH0328784.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDENIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6261..7331
                     /gene="disA"
                     /locus_tag="KR314_002408"
     CDS             6261..7331
                     /gene="disA"
                     /locus_tag="KR314_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBH0328785.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7420..8514
                     /locus_tag="KR314_002409"
     CDS             7420..8514
                     /locus_tag="KR314_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBH0328786.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8643..10820
                     /locus_tag="KR314_002410"
     CDS             8643..10820
                     /locus_tag="KR314_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328787.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDMSTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSENGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVVFVELGDTYRLDLYKPNLNEKTYESVKDNIEKNIDVYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVLFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
BASE COUNT         4551 a         1098 c         2020 g         3219 t
ORIGIN      
        1 aaatgtttag taataaagat gagaaggtat atttaaaata taaagtattt aaagttctga
       61 aatctaatca tgaagcaaaa aattagcatt tggttatcaa taaatataat aaataaaaac
      121 tcataattaa tgaaagaata aattcatatc taaatttcta taaaaaattt aaatttatac
      181 aatttagggc tttaaaaatt taaataatag gtgtaaaata atagtcaaag aaagtcaaaa
      241 ataaaaaggt gtgtggtgat attaatggca actatgacag atataattga aaaatttatt
      301 aaagatttga tggaagaaga taatagcata caaattcaaa gaaatgaact agcaaacttg
      361 tttagttgtg caccatcaca aataaattat gtcttaacaa caagatttac aatagataga
      421 ggatattaca ttgaaagtaa aaaaggtggc ggaggatatg ttcaaataga aaaaatacga
      481 aaaagtaaag atggacatat tagagaacta ctaaatgaaa aaataggaag tcagatatcc
      541 tataaaaaag caaaggaatt attagaaggt ttaaaagagt cggatttgat taacgaaaga
      601 gaattaaaat taatattata tgcaatagat gataaatcat tgtgtatgcc aatatatgaa
      661 ttaaaagaga aggtaaggtc taatatttta aagaatataa taataggatt atttagtata
      721 gaggagtgat aatttatgct atgtcaaaaa tgcaataaga ataaagcatc tgtatattat
      781 aataagatag tcaatggaga aaaaacagag atgtatttat gtagtgagtg tgcaaaagag
      841 aatacagaaa tgaattttaa cttagatatg cctttttcaa tgatggatat attttctaat
      901 ttaggttttc aacctaaaaa agaacttgag gaaaagctag tttgtcctaa atgcaataca
      961 acatatagtg agtttaagaa taatgggaga tttggatgta gtgaatgtta taatgcattt
     1021 agtagtcaag taaatccaat gcttcaaaat atacatgggc atatagaaca tacaggaaaa
     1081 gcacctaaaa aatcttttta taagataagt gtagaaaatg agattaaaga actaaaagaa
     1141 gatttagata gagctataaa aaatgaagag tatgaattag cagctcaatt tagagataaa
     1201 attaaatatt taaaaggttc tattgactaa gattggagtg atattatgaa agagaacata
     1261 gtcatgaaat caagagttag gctagctaga aatttaaata actatccatt tccaaataaa
     1321 ttagataaag aatgtgccat ggaaataata gaaaaagtaa aaaatgcgtt tattaattct
     1381 aacttagaac aaaaagaaga atttgatttc tataaaattg aagatttaga ccaatctaaa
     1441 aaaatgctta tggttgaaga acatataata agcccagatt tagcagaaaa tgataaaagt
     1501 gcagttatag taaaaaaaga taaaactata agcataatga taaatgaaga agaccatata
     1561 aggatacaaa ctatatgtga tgatttaaac ttagaatatg catatagtgt agcaaatgaa
     1621 atagatgatt tgttagaatc aagtttagaa tatgccttta acacaaagct aggttatcta
     1681 acatcttgtc caactaatac aggaacagga atgagagctt ctgtaatgat gcatttacca
     1741 gcattaagtc aactaggcta tatggatgaa ctttataaaa tttcatcaca aataggtata
     1801 gcaattagag ggatatatgg agaacgaaca gaagcattgg gaaatatata tcaaatatct
     1861 aatcaactta cacttggaag aacagagtct aatattatag aaaatgtaag tggacttaca
     1921 aaagatgcaa tttcaaaaga aataaaagct agagaaatat tgcaaaaaaa attaggtata
     1981 aaactagaag ataaaatatt tagatcgata gggacacttg aaaattcaag agtaatgagt
     2041 agcgcagagg caatgagcca tctatcaaat ataaaaatgg gaatagaaat gaactatata
     2101 gataaactag atttaagggc tatagaacaa cttatgatag gtatacaacc agcgcatcag
     2161 tcaataatgt ataagagtga tgatgttgag aatagagata taaatagagc aacatatatt
     2221 agagaaactt tggaaaaatt aagaggaggg atgaattgtg aacttcaata gatttacaca
     2281 aagagctaaa aaggcaattg atttagcgtt tgaatctgct aaaagtctag gacacaatat
     2341 tgttggaagt gaacatatac ttttaggtct tttaagagaa gaagaaggta tagctgctaa
     2401 agttctaagt aaagttggat ttacagaagc ttatttagaa ggcaaaatag ttgatatgga
     2461 aggtaaggga gaagaaatct cagaagatat agtattaagt ccaaggagta aacaaatact
     2521 tgaattatca ggaatgtttg caaataaatt aaaaacaaat tatattggga ctgagcatat
     2581 attattagca attattcaag aaggtgaagg aatagctaat aagattttaa attatgctgg
     2641 agtaaatgat aggactttag cccaattaac aattgatatg atgggtatta gtgataaaaa
     2701 tcaatacaaa gcagagaata gttacacggg caaccagaat caagcagaat ctaaagtact
     2761 tgataagtat ggaagaaatc ttacactata tgcaaaacag aataaaatag accctgtaat
     2821 aggtagagaa aaagaaatac aaagagtaat acaaatatta agtagaagaa ctaaaaacaa
     2881 tccagtgttg ataggagacc caggtgtagg gaaaacagcc atagctgaag gtctggctac
     2941 aaatatagcc ttaggaaatg ttccagaaac actaaaaaac aaaactttat actctttaga
     3001 gatgggttca ttattggctg gggctaagta tagaggagaa tttgaagaaa gaattaaaga
     3061 agttgtagat gaagttgtta aaaatggaaa cataatctta tttatagatg agatgcacac
     3121 aataataggg gctgggtcta ctggagaagg ctctatagat gcttcaaata tattaaagcc
     3181 agccttggca agaggagaaa tacaagttat aggtgcaact acaattgatg aatatagaaa
     3241 gcatgttgaa aaagattctg ctctagaaag aagattccaa ccagttatgg tagatgaacc
     3301 aactaaagag gactcaataa aaatattaga aggattaaga gataaatatg aagctcatca
     3361 taaagttaaa ataactgatg atgctataaa ggcagctgta gaattatcaa ctagatatat
     3421 atcagacaga tatctgccag ataaagctat agacttgatt gatgaagctg catcaaaagt
     3481 aagattaaaa gaaaatacac ctccttcaga aataaaaaaa ttagaactag aaatagaaaa
     3541 tatagataaa gaaaaagaag aagcagtaag atgtcaagac tttgagaaag cagccaaaat
     3601 aagagatgaa caaggtctac ttaaaaaaca attagaagat gttagagaaa gatggaacaa
     3661 gtcatctaaa cattcagatt tagttgatgg agaagttatt gctgaagtag tgggcttatg
     3721 gacaggaata ccagttaata aaattcttga agaagaggct gatagacttt tgagacttga
     3781 ggaaatactg cacaatagag ttataggtca agaacaagca gtaaaatcta tttcaaaggc
     3841 aatcagaagg tcaagagcag gtcttaaaga ccctaataga ccaattggtt catttttatt
     3901 tttaggtcca acaggagtag gtaaaacaga attatctaag gcattagcag aagtgcaatt
     3961 tggagatgaa aatcaaataa ttagaataga tatgtctgaa tatatggaaa aacatgctgt
     4021 gtcaagaatg ataggttcac ctccaggtta tgtaggtcat gatgaaggtg gtcaattaac
     4081 tgaaaaagta agaagaaatc catattcagt tatcttattt gatgagatag aaaaagctca
     4141 tccagatgta tttaatattc tgttacaaat tctagatgat ggtagactaa ctgattcaaa
     4201 aggaagaact gtagatttta agaatactat tgtaataatg acatcaaatg ttggagcatc
     4261 tacaattggt agacaaaaaa ctttaggatt tagtatagct aaaggagacg aagaagaaaa
     4321 atctcaatat gaaaaaatga aagaaaatat aatgggtgaa ttaaaacaaa gattcagacc
     4381 agagttttta aacagaatag atgatataat tgttttccac tcactaaatg aaaatcatat
     4441 atcaaaaata gtacttttaa tggcagcgaa actacaagag agattaaaag aaatggatat
     4501 aaaattagaa atgagtgatg aggctgttaa attaatttct aaatctggat ttgacttaga
     4561 atatggagca agacctctta aaagagcttt acaaaaagag ttggaagatg agctatctga
     4621 agcaatctta aaaggtaatg tgaaaaaggg aagtaatgta gtagcaaaag tcaaagatga
     4681 aaaaatagtt tttgaaacta aataatttaa tagagttgca ttttaaaata ttgcaaatat
     4741 aagttttata atctaaacaa aattttaagg gcaatgaatc tataataatt cattgtcctt
     4801 aattatttta tttaatcttt acatattgtc tttcagatat gctaaaatta tagtatagaa
     4861 taaaaaatta aggatgataa aagtatggca aaaataaaaa caaaatatgt atgtcaatct
     4921 tgtggatatg aaacagctaa atggcttggt aaatgtccag aatgtacaaa atggaataca
     4981 tttgtagaag aaatagacca aaaaagcacc aaaaaagagg tctttataat agataaatct
     5041 tcatctaaac cagtcagtat aaactcaata gaaagtaaag aagaagagag gtttacaaca
     5101 gatataaatg aattagatag agtgcttgga ggaggtatag ttaaaggctc tttagtgctt
     5161 gttggtggtg accctggtat aggtaaatca acacttttaa tccaagtatc tagtaatgtt
     5221 gccaatttag gaaaaactgt tttatatata actggagaag aatcagaatc tcaaataaaa
     5281 atgagagcta aaagattagg tattaactca gaaaatttat atatatttgc tgaaaataat
     5341 ttaagtataa ttgaatctta tttagaaagt gtaaatccag aattaataat tctagactca
     5401 attcaaactg tcttcagtcc agaaatatcc tcggcaccag gaactgtcag ccaaattaaa
     5461 gaaggtactt caaaattcat gaaaatttct aaaaaaatgg gaatttcaac gttcatagtt
     5521 gggcatgtta caaaagaagg ttcactagca ggaccaaaat tattagagca tatggttgat
     5581 actgttctat attttgaagg agaaagatat aatacttata gattagtaag agctgttaaa
     5641 aatcgatttg gttcaactaa tgaacttgga gtatttgaaa tgagggattt aggattagta
     5701 gaacttgata atccatctaa aattttaatc tctgaaaaac caaaagatgt agcaggttca
     5761 gtaataatat ctactgtaga aggtacgaga ccaatgttac ttgaattgca agcattagtt
     5821 tctcctacaa gttttggtat accaaaaaga acatctacag gagtcgatta caatagagta
     5881 ggtatgttat tagcagtatt agaaaaacgt gttggtttgc aaatacaaaa tcaagatgtc
     5941 tatataaaca tagttggagg aataaagata aatgaaccct caattgattt agggatagcc
     6001 atttctgtag catctagttt tagaaatata ccgatagatg aaaatatagc tgttacagga
     6061 gaagtgggac ttacaggaga agttagagca gtaagcttta tagaaaagag aatagctgag
     6121 tgtaaaaaac ttggatttaa aaagatagta gttcctagaa gtaattatga tgttgtaaaa
     6181 gagaccaaag gcatagaaat atggccagta gataacttaa gacaagctat aaatatagta
     6241 cttgggagga atcaataaat atggagaatt ttctagataa taaaaatatg ctatatgcat
     6301 taaaaatgat atctcctgga actccactta gattaggtct aaacaatgta ctaagagcta
     6361 agactggtgg attaattgta attgcaacaa acgaagatgt aatgaaaata gtagatggag
     6421 gatttgctat aaatgcagaa tattcaccat catatctata tgaattagct aaaatggatg
     6481 gagctatagt tttaagtggt gatgtaaaga aaatattatt tgctaatgca caacttatac
     6541 ctgactattt tatagaaaca tcagagacag gaacaagaca tagaacagca gaaagagtag
     6601 caaaacaaac tggtgctata gtcataggaa tttcacaaag aagaaatgtt ataacagttt
     6661 atagaggaaa tgagaagtat gtagtcgaag atatatctaa gatatttact aaggcaaatc
     6721 aggctataca aactctggaa aaatataaga cagtattgga ccaagctgta acaaatttaa
     6781 atgccttaga gtttaatgat ttggtaacta tttatgatgt tgcattagtc atgcaaaaga
     6841 tggaaatggt aatgagagtt acaagtataa ttgaaaaata tgtgatagaa ttgggtgatg
     6901 aaggaacttt agtaagtatg caattagaag aattaatggg tacaaccaga atagaccaga
     6961 aattaatatt caaagattat aataaagaaa acacagaaat aaaagaactt atgaaaaagg
     7021 tcaaaaattt aaattcagaa gaactaatag aattggtcaa tatggcaaaa ctattagggt
     7081 atagtggttt ttcagaaagt atggatatgc ctataaaaac aagaggttat aggattctta
     7141 gcaaaataca tagactacca acagcaataa tagaaaactt agtaaattat tttgaaaact
     7201 ttcaacaaat tttagatgca tctattgaag aattagatga ggttgaagga ataggtgaaa
     7261 taagagcaac atatataaaa aatggactca taaaaatgaa acaattagtc ttattagata
     7321 gacacatatg atgaatttta aatgaatttt actacttttt tcttgaatat aatatacact
     7381 ttggtctata atagataagt ataactaggg ggtgaagcat tgataaggaa agtaacaaga
     7441 atattgttta ttttacttgg atttacaata ggtataacaa cctatttaac attaatgaaa
     7501 gattttgaaa tattaacatt tggaaaagaa acatatggtt atatcgcagc tgtagtagca
     7561 ggtataataa tagccatttt aggatatctt atagaaccat gggttgtaaa taaagtaaaa
     7621 gaaattgcta aaatagtgga taaagaattg tcaaaatatc ctcagactga tatattgtta
     7681 gggtctatgg gtcttatagt aggatttgtt attgcgtatt tattaagtgg attagtaaat
     7741 agaataccaa tagtaggtgg aatactatcc ttactattat atttattcct aggatatctt
     7801 ggaatgaaag ttgcgcttaa aagtaaaaat gatttattta atattggaaa actaggaaga
     7861 ttagcaaatc ctattaagga taaagataag gataaagaaa ataaaaaaga agtaaaagcc
     7921 attcctccaa aagtattaga tacaagtgta ataattgatg gaagaatagc tgatatttgt
     7981 aagactgggt ttatagaagg aaaacttatt attcctgctt ttgttttgga ggaactaaga
     8041 catattgcag attcttcaga tgatttaaaa agagtaagag gtagaagagg tctggatata
     8101 ttaaatataa ttcagaaaga attaaatata gaagttgaaa taagtgagag agactttgat
     8161 gatatagcag aagttgatag taaattatta aagcttgctc aggttttaaa tggaaaagtt
     8221 gttacaaatg attataactt aaataaagtt gctcaattcc aaggtgttga agtattaaat
     8281 ataaatgagt tagctaatgc tataaaacca gttgctatac caggtgaaga tatggttgtt
     8341 caagtggtta aagaaggaaa agaggctcaa caaggtgttg catatctaga tgatggaact
     8401 atgatagttg ttgatggagg aagaaaatat atgaatgaga ctataaaagt actcgttact
     8461 tctgttcttc aaactcctgc aggtagaatg atatttgcaa aacctaaaaa ttaatatgtt
     8521 caaagaaacc tataatttgg gctgtttatt ttaaatagat ggtccaaatt tatttattta
     8581 taacgataat gtttaatgta ttcttaaaga tggggattta aatcagaaag cagggagaat
     8641 ttatgagaaa aaagattata tctttcttag caacatttat cataatatta acatcagcaa
     8701 gtcagtatag ttttgcagat gatatgtcaa cacgtggaaa agtaatattt atagatatga
     8761 atagaactag tatgagtaat atgttaagaa taaaatcatt aagagaagaa ctagacaata
     8821 gagggtatat tggcttgatg aatataagag gcgataaagg aagtgatgat agaaggtcat
     8881 atgctagtat gggtgctggt ggtagagcta atgtggctaa tgaagaagat attaattttg
     8941 aaagctcgag taaggataga aatatagttt ttgaatcagc tacaggaaaa agtgcaaaag
     9001 gtataaataa tttaactata aataagtcta taaatgaaaa tttaaacttt ggagagtatg
     9061 gttctgtatt agggtcatta ggacaaagtt tatctgagaa tggattaaaa gcatctgtat
     9121 tgggtaattc agatataatt gaaaatggtc aacttataaa aaatagaaac ttatgtctaa
     9181 ctgcaatgga tgaatatgga agaataccta atggaaatgt agatactata aataaaaaag
     9241 acttatctat gccttatgga atatcaactg attatgataa attgattgta gagaccaaag
     9301 aagcttacaa aaataatgat gtggtttttg tggagcttgg agacacatat agattagatt
     9361 tatataaacc aaatttaaat gaaaaaacat atgagagtgt gaaagataac atagaaaaaa
     9421 atatagatgt atatttaaaa aatatatttt ctatggttga agaaaatgac actgtctaca
     9481 tagcaagtgc atttccaagt gatttagatt ataaaaataa aagaagactt tctccaataa
     9541 taaaattaaa tggagaaggg aaaggtatat tatcatcttc aactactaga agagaaggta
     9601 ttgtaacaaa tttagatgtt ggggttgaaa tattagacaa ctttaatata aaaaatcaaa
     9661 atatggttgg aagaaaatat gaacttatta acagagatga taataaagaa tttttgatgg
     9721 atgaatatca aaagatagta tctatttcaa gcataaggtc tacaatactg aatggttttg
     9781 taagtatagt tttcttgtca tggatagttg ctatgattgc aattttattt agaaagcata
     9841 tatcaaaaaa ttataaagaa acaacatttt ttatattaaa ggaactttta aaaataggaa
     9901 tagtaatgcc actgtcattt atgataacac caataatgaa tttcaaaaca ccattagcaa
     9961 tatctttagg aataattata ataacactta ctatatatct tataagtaaa gttttaataa
    10021 agaatgattt aaaaaacatg ctgtttttta ctggattaac catagtaatt atggttattg
    10081 atgctgggtt tggctcatat ttaatgaaaa gcaatgttat gagttatgac tgtataattg
    10141 gagctagata ttatggagtg ggaaatgagt atcaaggtgt ggcaatcggt agtgcaatat
    10201 ttacatttgc aattttactt acatataaaa atataccaaa gtggtctata atagtttttt
    10261 ctttggttat cttaattaca agtgcatctc caattatggg agcaaatgtt ggaagtgcta
    10321 tttctgaatg tgttgcattt ttactattta tactattgat atataatgtg aagatagact
    10381 tcaaaaaaat tgtattatta ggtatagcag tattatttgt attaggagtt tttgtagcaa
    10441 tagatatgat tttgggaagt aattcacatt taggtatgtt tgtaaaagaa atttacttta
    10501 atggaccagg agaaataata caaacattta gtagaaagat agagatgaat ttaaagctag
    10561 ctcaaaccag tgcatgggta aatatactgt taactggaat aggtgtaatt ttagttctta
    10621 tgattaatca gattagatac tttaagcaac tgatggatga gtatccaatt gtatttaaag
    10681 gatttatagc atcaatagca ggatgtttag taactctttt agtgaatgac tctgggattg
    10741 tgtcatcagc tacagccttt atttatgtaa ttgtacctat gataacatta agtataaatc
    10801 tcacagcatt aaaagaataa gtactgtatg aataatatgt aaatatgaac acattaattt
    10861 aacttcttta tttacaaaat aaagaaga
//
LOCUS       DAEPLF010000115        10857 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEPLF010000115 DAEPLF010000000
VERSION     DAEPLF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10857
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.115"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..1811)
                     /locus_tag="KR314_002411"
     CDS             complement(<1..1811)
                     /locus_tag="KR314_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBH0328788.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKNKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDYVVSKKVDEIKEQQEKILDKVIGATTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGERITSIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPILKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNQGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFN"
     gene            complement(2024..2908)
                     /locus_tag="KR314_002412"
     CDS             complement(2024..2908)
                     /locus_tag="KR314_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBH0328789.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRIKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGKKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            3116..4033
                     /locus_tag="KR314_002413"
     CDS             3116..4033
                     /locus_tag="KR314_002413"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBH0328790.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLNSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            complement(4162..5496)
                     /locus_tag="KR314_002414"
     CDS             complement(4162..5496)
                     /locus_tag="KR314_002414"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBH0328791.1"
                     /translation="MRVVIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFDDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            5838..7226
                     /locus_tag="KR314_002415"
     CDS             5838..7226
                     /locus_tag="KR314_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBH0328792.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFTIHLIFITLNGLNPITYIRNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSLYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFGKKNIS"
     gene            complement(7307..8515)
                     /locus_tag="KR314_002416"
     CDS             complement(7307..8515)
                     /locus_tag="KR314_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328793.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNICYSGKDMIQEIKNSVAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSEEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKENYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCK"
     gene            complement(8530..9948)
                     /locus_tag="KR314_002417"
     CDS             complement(8530..9948)
                     /locus_tag="KR314_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBH0328794.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCVYNIGVLFIFKYLAFAL
                     RNISELINTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTMQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMLGG
                     NGVIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            complement(9966..>10857)
                     /locus_tag="KR314_002418"
     CDS             complement(9966..>10857)
                     /locus_tag="KR314_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328795.1"
                     /translation="DSSKSDKERYLYIYQNIKENWSMYNNFYIEIQNKNKSSQKINLS
                     IQSKNMLEFRLKEGSEIFLEDKNIIYSDKIKEGCIEVPGEFEGKIYVNFNSLINEKSN
                     VVLDSSMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKLSFLHNIKITGNEEVQIP
                     VLGQSISQYEVLGLKRDSKIKYSLMGKQDNVSISQNGKLTLSNKAEPGQIILQVNVDD
                     KFKIGRKIALTESWSINKKDKDGVPYTLVSPEQSPTVQYMKKINFMNNIITFVRILFV
                     SLVIICFGIYLYWKKCSKTK"
BASE COUNT         3635 a         1819 c         1205 g         4198 t
ORIGIN      
        1 tgattaaaat tcaataaaca tatcaatgta cttgaaacta taaatataat tatactattt
       61 gaaaaaccta aatctcctgt tttaggagat ttttctttag cttctctttt agatattcct
      121 tgattagaac tttctctagc acttgtttta tctaagcttt tatttttgtc actatcagag
      181 ctttttatat tattagatag attctcttca ttatcattga tatttttttc attttcactt
      241 aatatcaaat cactctcatg attcttgctt attaactcat ctttagattt aataccaatt
      301 cttccatcta cattttttga tatttctttt ttgtactcta tatattcaat gactgcactc
      361 tctaaatttc caaattcctt tagtattggc tcttttgaaa aacaaggaaa ttcatctcca
      421 cctgaggcta tataatcatt cgttgcaact acatactttt tatttaaatc taatggcttt
      481 ccttcttttt gaatacttgt tattctttcg ccttcttttt gtttaggatt aaagtagtat
      541 gaaattcctc ccacttgcaa aaaggctgaa cttttttctg gataaagttt tactccatgc
      601 tccaatacat ctttaatctg tgctcctgta agttctttgg taactatagt attgctaaaa
      661 ggaaatacat ctacaatatc tcttcttgtt atattgcctt tttctatcgt atctcgaata
      721 ttccctccat taaataaaga aatatcagcc ttagttttat ctagtaaaat gtcacttatt
      781 aaattaccca aattagtttc ttttgtcctt acattttcat atgaaccatc tagagtattt
      841 gtagttgcac ctataacttt atctaatatt ttttcttgtt gttcttttat ttcatcaacc
      901 tttttactta caacgtagtc aagcttatac tttaatgctt cttcttttcc aattaatcta
      961 actgatttat tttctatttt tatattttct acatcattag tatccaaagt tagttttatc
     1021 tcgccaatat tttctaaata ttgtcctgca cttacaatca atgtatcacc cacttgttta
     1081 ccattttcaa acttagtgtg gctgtgtcca tcaataatca aatcaattcc ttgaacctca
     1141 tttgctatat ctatactatt tggcttactt aaactatttg taccaatatg acatattgca
     1201 actattacat tggcaccttt tccttttaat tcatttactt gttctgttgc actttctata
     1261 ggatttttaa actctaatcc ttccacattt ttaaatccag ttttgttctt tgtttcctgg
     1321 gttgagagtc caaaaaatcc tatttttatt ccatctattt cttttatatc attactatca
     1381 aaaacttttt ttccattttc aaaaatattt gatgataaaa attttaaagt attctctcct
     1441 aaattagcac ttttagataa ctcaaacagt ctatcttttc cataattaaa gtcatgattt
     1501 ccaggagtaa tatagtcata tccagcacat ttaattaatt caattgcact tttccctcta
     1561 tccatattta ctattggaag tccatgcaaa caatctcctg catctactaa aattgagttt
     1621 ggagtttctt ttttcaatgt ggctagattt cctatttgta tatggtcatc accttcagca
     1681 tatcttccat gtatatcatt agtgtgaaaa atggttattt cttcaaatcg ctctagccca
     1741 taagaaataa aactctgact tgttgtaatt agaattataa ttaccaaaat taaatacctt
     1801 ctaatcttca atataaatta gcctcctcaa attagtttta gctttaatat aaaatttata
     1861 ttttacataa aactttttag ataaatatta aactatagta agattgatta gtgtaatttt
     1921 taatatttat ctatcaattt aattttgtcc atatccaatt agttttaatc taattacatt
     1981 ttttcacatc tatatttttc ttttattata taaaattatt attttaaaat cttttcaact
     2041 ggtctctttt aaaagtccat tgaggtattg tcaaatcacc ttcaaaatct aaactatacc
     2101 aaggactcaa atttggattt ttacgattaa taagaagttg cgtttgttgt gcaggcatat
     2161 agctttgggc tatcataaat acctttttac cagtcttttc attttctgcc atatccataa
     2221 ttatacttgc atgtcctgga cttccaccaa ctataaacac atctcctatt ttcatatcct
     2281 caacttttac aggctccaat tctttttcaa gagataacgt tcctgaatat gcaaacacta
     2341 tatccataaa ctttctgaaa ctctcataag atgttgaata ttcagttgct ttataccagc
     2401 ttgaaccact atcttttact gaaattctat atccttgtgc ccattttgaa tacttcgcct
     2461 taaaaccatc aacaaaatca aaacttattt catcatatag tttattctga tacaagtatt
     2521 ctgctctcat aagcataatc gcatctgcac attgatgcaa atctctattg ccaatatcaa
     2581 catcaaatac actatcataa ataccttttg attccttaat tcttccatca taatacttta
     2641 ctttctctcc ataatttttt agcttagtat tcctaagaaa tgattcaaaa ctaccttttt
     2701 ctacatcaac tcttttaaat ccttttggtg gctcatacct atctttcaat atatttgcat
     2761 ctttatgtac tattataggc tgttcatctt ttttagaatt ttgaattttc ttagaacctt
     2821 caaccacttt tttatcttct ccgttactac aaccaactaa ggttaatgta atcaatgaca
     2881 aaatagtaat tttctttaat actttcatta aattttcctc ctttaaatac tcctaaatat
     2941 aattttcact tttcgttctc caaattttta tacattgcta tataaaaact ttatttgata
     3001 atatttcttg actcaaatac acatttattg ttatatttaa tgttaaaata attataatag
     3061 gatatcataa taaaaacaaa caacagattt gtgttaattt tagaaagggg aaaaaatgat
     3121 agatttaact ttacttggat gtggtggaaa tgtacctatg ccaaacagat ttctatcctc
     3181 tgtatttata aactataaag gtagaaaaat actaattgac tgtggtgaag gtactcaggt
     3241 atctatgaag ctaaaaaaat gtggctttaa agatatagac ttgatttgta taacccattt
     3301 acatggtgac catatatttg gacttttagg acttttatca acaattggga atagtggtcg
     3361 tacaagtgat ttaactatag ttggtccagt tggaatagtc gattgtatac gttctatgag
     3421 aaatttagta gaatatgtac catacacttt aaaaattata gaaaaccctc aaggcaattt
     3481 ttctttagat aataaagttt taagaaattt agaaatttca acaatcagtt tagaacactc
     3541 tatagagtgt ttaggataca gttttaattt tagaagaaac cctaaatttg atatagataa
     3601 ggcaactaaa aacgaagttc ctaaaatatt atggaacaaa ctccaagaag gtcaaaatat
     3661 agttttaaat tcaaaacaat acactccaga tatggtctta ggagaattga gaaagggagt
     3721 taagataagt cttacaaccg atacaagacc tatagaatca ataccagatt tcataaaaga
     3781 tagtgattta tttatatgtg aagccatgta tggtgatgat ttagatatat caaaagctgt
     3841 tagaaacaaa catatgactt ttagagaagc tgctaatctt gcaaaacttg gaaatgtcaa
     3901 acaactttta ctgacccatt ttagtccttc tttagacata cctagtatgt atttagaaaa
     3961 tgctactaat gtttttgaaa atactattct tggagaagat agattaagct tgcgtttaaa
     4021 ttttgatgaa taatttaatg gaatccttat ttctaaaaat ttggaataaa aatttaggga
     4081 tgaattttac catgataaaa atagtttagg taaattcatt cccttaattg cttgtcaact
     4141 atattagcta taatctaaat attatttaga cacatttcca gctacattta aaacatccca
     4201 tgttcttgca aatggtgggg cataacataa atctaacatt ccaagttgac ttgtggtcat
     4261 ttttccaaag atagcagctg ccaatacatt tgctctttgg acagcatcct taaacccagc
     4321 cacttgtccg cctaaaataa cttttgtatc tgcatcataa ataagtttta catatatttt
     4381 atttcttcct ggataataat gagtttgatt ataatcagaa ataaatttag atttgacatt
     4441 tatgcctaaa tctttagctt gcttttctgt aattccagtt gatgctgcct ccatatccat
     4501 aatttttata caacttgaag atagtgaacc ttgataagat acttctcttc ctgctaggtt
     4561 ttctcctact atcctaccta atttattagc tccagtagct aaaggaacat atacattttc
     4621 attacttact atattcttta tagttgcaca atctccagca gagtatatat cttctacaga
     4681 agttcttcca tattcatcaa ctactatagc tccatttttt agcattttta tattagtatt
     4741 tgacaaaaac tcggtattag gtctcacacc agtagcaatt attactacat ctgtatctat
     4801 ttcccctttg tttgttataa ctttttctac tttatcttct ccacaaagtt ctacaaccat
     4861 ttcatccaag tgcaaattaa cattatgtct atttatttct tcttctaata catccgtcat
     4921 ctccttatca aatacttgtg gcaatattct atcttctaac tgaattacat ctacatcttt
     4981 acctaatttc ttacatgcct caactgcttc taaccctata aaaccagctc ctattattgt
     5041 cactctttta ttatcttcat tacttaaaag tttttttaga tattctccat catataaact
     5101 ttttaatgta gaaacattct tcaattttat gttttttata ggaggcataa tacttcttgc
     5161 tccagttgct atcatcaatt tatcatagta atcttcatat atttcatctg tttttatatt
     5221 ttttactttt attttcttag attcactatc aacctcaacg acttctctaa atatatttaa
     5281 atcaattcca gcttctctaa gtttctctgg tgttcttgct aataattcat cagcatcatc
     5341 aaaaaaacca ccaacaaagt atggtaatcc acaagcacca aaagatacta tttcagtttt
     5401 ttcataaaca acaacttcat actcaggttt tattcttttt aattttgctg cagcactcat
     5461 accagctgca acaccaccaa ttattactac tctcatttta tctctccttt gatgtctagt
     5521 taatttcttc acttatttta agcttttcat ttgatatata ttctatgtaa acatttttca
     5581 taacaatttt tagttattta tttaacatat tttaatttga aatcaaatat tataaaagct
     5641 attttttatt ttcttaccac aattcaaatt ctttaaacat ttattatacc ctatttttct
     5701 atacttaatc acatttttca aaatcaattt attttactct atttaagtaa attgtgatat
     5761 aatattgaca tttttttaat atattggtat catttaaact tagaaaaatt tatttttaaa
     5821 taaaaggaga aaaaatattg gaaagttcat ttttatcaca atttcttatg attacagatt
     5881 ttaaaacgat ttttttcata atattacttc taggtacatt ctttattgtt agacaatttg
     5941 aaaagaaaaa aataaaattt tcattgagaa caatatatgc tacagtacta ggactcatat
     6001 tgggaatcat aatacaagct tttgctggtt ttcctaatga taccactcaa gtcacttggc
     6061 tacaagaagt aagcaagtgg tatagcctat ttggttctgg atttatggac ttattaaaaa
     6121 tgttagtagt acctttagtt ttcttatcca ttataaaagt aattataaat atgaaagata
     6181 acaatcttgg aactctgaca tttaaatctt taggtatgct tcttgctact actgctatag
     6241 ctgcaattgt tggaattatc gttgcaaata taatgaagtt aggtgtagga gctaatatgc
     6301 ctactttagt tgaaaatcaa gaacttaaag aaataaactc tcttgtggat actttaagag
     6361 gattactacc atctaatcct gtaatggcta tggcaaatgg aaatattgta gctacaataa
     6421 tatttgctac atttatagct acctcaataa agagattaag taaaaaacac tttgaaacaa
     6481 taaaaccatt tatagatttt atagaagcat cttacaaaat aattgtaagt gtgtctatga
     6541 ctgttattaa atttatgcct tatgctgtgg ttgctctgct tgcaaataca ataacttcac
     6601 aaggtattag ctcaatagta tctgttttac agtttatatt agctctttat atatcagtgg
     6661 caatattatt taccatacat ttaattttta taactttaaa tggtctaaat cccattactt
     6721 atataagaaa ttcaactgaa gctttacttc tggcatttac ttcacgttct agtttaggaa
     6781 cattgcctgt aacgatagaa agtctagtac aaaatcacgg agttgatgag ggtgtatcaa
     6841 gctttacagc aagtttaggt gcaaatatgg gtatgaatgg ttgtgcaggt atatatcctg
     6901 ctttaatggc tgtaacactt gccaatatgg ctggagtaaa catggatatg agcctttatg
     6961 ttatgttgtt aattgttata acaattagtt cgtttggaat agctggtctt ccaggaactg
     7021 ctacaatggc cgtttcagtt gttatctctg gagttggtct tggttcttat tttccattag
     7081 ttggtggtat acttgcaata gaccctatac tagatatggg acgtaccatg ataaatgtta
     7141 atggtgctat ggtatcatca attactgtag gaaaatcact tggaaaaata gataaagaag
     7201 tatttggtaa aaaaaacatt tcttagagta acacttaaga taaatggcta acttttaact
     7261 agttagccat ttatttttta ttttataaga aaatttcttt agaaatttac ttacaaaacg
     7321 gaaaaaattc ttttgtaaga ttttggggat atacagatat aaaaacaaag tcatagtcat
     7381 gggattttgc atattctagt atatctccat cataatatct agggtctatc atatctatag
     7441 atgaacaaac agaggaaaaa aatgctgctg ttggtacaat tagagaatct ttaacaaaca
     7501 gcacttttaa tccatctgga ttttctatat tttttatttt aactaatggt ctattcccca
     7561 ataaatatgt aaaatactta tctgattgac catccattaa atcaaggtct gcattaaaag
     7621 gatatgataa tattaaactc tcttcaaacc ttccttttaa ttcaaatttt tgagatttag
     7681 aatctgtata ataagtataa ttagtcttaa atttaggata aattaatgta aaatcatcta
     7741 taccaccata taagatacca gtctttcttc ccatagaacc aagaaatgac ttaggatata
     7801 caattgagtt ataattttct ttatctctat agtaatgatt ttcatctaaa ttcatattgt
     7861 attttttatt aagttgctca actaattctc caaatgccca aaaagaagtt tctactttcc
     7921 agtgatggtc tgttttaaaa aataaatcat cttttggaat accactcttt aaaatatttt
     7981 ctctaaaatc aatatagtcc acattatact tttttagttg atttaaaaaa ttatctgctg
     8041 tctcattgct ataagaataa ggtattcctt ttggaaattt tgtcactcct tttatatact
     8101 tatctggagt cattaagtaa actaatttag tgttttgctt ttccatttcc tcagaaaatt
     8161 ttcttaccct atctgctatc acaccaactg gatttggagc tttagcaaaa tatgtataat
     8221 gcatctttcc agaagtatct tttacaacac taaattttga aaactcattt ttacccatta
     8281 acttttgtaa gtagccataa gtttcaatga acgtattttt atatattact ttgtcattta
     8341 ctgtgttatc aatagatgat attgtatctt ttaaactact tttttcagca actgaatttt
     8401 ttatctcctg tatcatatct tttccagaat aacaaatatt aaagatagag aaagcgaata
     8461 aaataattaa gaacataatt gcagttatta atcttacttt aaaaaatcta ttcatacttt
     8521 tcctcccaac taaaaattaa aataaataaa tggattatag cttccagtta ctaaatatgt
     8581 cacagaaata aagaacaata aaatagctga gattggataa aatacgttaa atatcatact
     8641 atatttagct tgttcaacaa caaacttatt gattttcttt gcaataggta cactaaaaat
     8701 aaatgcaaat ataaagaaaa cccagtattc ttttaaaaac atatatgtat aatcactcca
     8761 tataactcca ttgcctccaa gcatagcttt gaaataatta tatgcttctt ttaaattagg
     8821 tgacctaaac aatacccacc caaagttaac tactaataac gcataaatat gttttataaa
     8881 attgtgattt tcaatctttt caaacattat aaatcgctct ataatcaaga aaacaaagtt
     8941 taaaatcccc caaattacaa atgtccattc tgctccatgc catattccag taaataacca
     9001 cactataaac atatttctaa tcataatatc tttatttgct actctagaac ctccaagcgg
     9061 aaagtatata tagctcttaa accacattcc caaagaaatg tgccatcttc tccaaaactc
     9121 actaattgat tttgatatat atggataatt aaagttctct tcaaatttaa aaccaaacat
     9181 aagacctaaa cctatagcca tatctgaata agcagaaaaa tcaaagaata tttgcatagt
     9241 ataggctatt gaaccaagcc atgcaagtga tgcagctatt tctccttgac tattcatagt
     9301 gtaaatatag tctgctacta tagccatgtt atttgaaatt aatacttttt tgcctagacc
     9361 tgttataaac ctgcaagtcc caatagaaaa tttattccaa gtttcttttc tattgagaat
     9421 ttgttctgcc acactctcat atctaactat aggacctgct attaattgtg ggaaaaatgc
     9481 tatatacagt cccacataaa aaggattctt ttggacttct ccatgtctac gatatacatc
     9541 tataacataa gacattccct gaaatgtaaa aaatgaaatt cctataggta aaacaatact
     9601 aggtatagtt agttcagtat taataagttc actaatattt cttagcgcaa aagctagata
     9661 tttaaatata aataaaacac ctatattata tacacacatc aaaactaaaa aaacttttac
     9721 ttttatttta ctttccctat atctatctac tagtaaacca aatatatagt tcattattat
     9781 ggaaactatc atgataacaa caaattttgg ctctccccaa gcataaaaaa ataaactaac
     9841 agcaagcaat atcatgttct gtatcgttcg gttaaaccta aatacataat ataaaaccaa
     9901 agttataggt aaaaaataaa ataaaaaaat taaacttgaa aataacacaa tctaacctcc
     9961 tctctttact tagtttttga acattttttc caatataaat atattccaaa gcatattatt
    10021 actagagata caaataaaat tcgaacaaac gttatgatgt tattcataaa gtttattttt
    10081 ttcatatatt gtactgttgg tgattgctca ggagatacca atgtatatgg aacaccatcc
    10141 ttatcttttt tattaatact ccaagattca gttaatgcta ttttccttcc aattttaaac
    10201 ttatcatcaa cattaacttg taaaataatt tgtccaggtt cggctttatt acttaatgtt
    10261 aatttaccat tttgagatat gctcacatta tcttgttttc ccataagaga atacttaatt
    10321 ttagaatcac gtttaagacc taatacctca tactgactta tactttgacc caatactggt
    10381 atttgtactt cttcattgcc agttatttta atattatgta aaaaacttaa tttctcttca
    10441 gacaataaag atatcttttt tataataaca atattatgtt cttcatcaga tggaataaat
    10501 gtaattcccc aactaacaat gtttgaaagc atacttgagt ccagcacaac attacttttc
    10561 tcatttataa ggctattaaa gttcacataa attttgcctt caaattcacc tggtacttct
    10621 atgcatcctt cttttatttt atctgaatat attatatttt tatcttccaa aaatatttca
    10681 ctaccttctt ttaacctaaa ttcaagcata ttcttacttt gtatacttaa atttattttc
    10741 tgggaacttt tatttttatt ttgaatttca atataaaaat tattatacat actccaattt
    10801 tcttttatat tttggtaaat atataaataa cgttccttat cacttttaga actatct
//
LOCUS       DAEPLF010000116        10615 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEPLF010000116 DAEPLF010000000
VERSION     DAEPLF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.116"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(162..869)
                     /locus_tag="KR314_002419"
     CDS             complement(162..869)
                     /locus_tag="KR314_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH0328796.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            complement(1068..2981)
                     /locus_tag="KR314_002420"
     CDS             complement(1068..2981)
                     /locus_tag="KR314_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBH0328797.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNSELKKVAVAIIDDDKDKIGRIIHNVKIVGTTSDIKAVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            complement(3065..4156)
                     /locus_tag="KR314_002421"
     CDS             complement(3065..4156)
                     /locus_tag="KR314_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0328798.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            complement(4162..5505)
                     /locus_tag="KR314_002422"
     CDS             complement(4162..5505)
                     /locus_tag="KR314_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBH0328799.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNNVK
                     VELASNPEFLSQGTAVKDTLYASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            complement(5483..6235)
                     /locus_tag="KR314_002423"
     CDS             complement(5483..6235)
                     /locus_tag="KR314_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0328800.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            complement(6283..7191)
                     /locus_tag="KR314_002424"
     CDS             complement(6283..7191)
                     /locus_tag="KR314_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0328801.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            complement(7213..7962)
                     /locus_tag="KR314_002425"
     CDS             complement(7213..7962)
                     /locus_tag="KR314_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0328802.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYKRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            complement(7991..9166)
                     /locus_tag="KR314_002426"
     CDS             complement(7991..9166)
                     /locus_tag="KR314_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBH0328803.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            complement(9181..10020)
                     /locus_tag="KR314_002427"
     CDS             complement(9181..10020)
                     /locus_tag="KR314_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0328804.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNEDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYTFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            complement(10023..>10615)
                     /locus_tag="KR314_002428"
     CDS             complement(10023..>10615)
                     /locus_tag="KR314_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBH0328805.1"
                     /translation="FGSAFFVYQTTYSRTGFFTILAGLIFVEIIRWIDIKKIKGLPML
                     LKTLPIILTLFSVIIGTIFDKSTLLNRLLASRPKFWHVYLAEKGNFLKPFGNSYSPAI
                     KATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKAYLVALFIFLLYSFAE
                     NLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
BASE COUNT         3303 a         1989 c         1048 g         4275 t
ORIGIN      
        1 ttattataaa aaaaacagca aagaaattta tcttctttgc tgtttttata acgttcatca
       61 ctattttaat attactaaac tcttaaatct ctatgtaaaa cattaaatgt aattatatat
      121 ttaatgttaa tattcaatcc tatgtaaatc aatatctatt attattgtaa aacacgtgta
      181 gcagttataa gtcttcttgc ataattagtt cctggagcca tgctagtaac ttttactaca
      241 tctcctgttt gtggtgaatg tatgaattta gaatctccta catacaatcc aacatgattt
      301 attgaaccac ctgaaccaaa gaaaactaaa tctcctggtt ttaattctgc tcttgatact
      361 ttctttccta ctgttgcttg tgatcttgat actcttggta aattaacccc tgctccattt
      421 ttatagacat aagaagtgaa cccagaacaa tcaaaagcat ttggtccagt tgctccccat
      481 ctatatggct taccttgttg cttaaatgct aagtttaaaa cattttgtac ctttgcatct
      541 acatttccac cttgaatagt taaataatct ccatttaccc atcctgtaac attgtcaaca
      601 ctttttattt taatccatcc atttgaatgt aattctaaaa cttcaacttt gtctcctgtg
      661 tttatctttc ctactactgc atcatttgca ctagctccac ttcttacatt taaaaaatca
      721 cactggtcta ctgttgcact cataggtgaa ttagttggct ctgctgcact agccgttatt
      781 gctcctgctt ctactgtgac aactcctgct attaacacca tagctataaa ttttttcaat
      841 atatgttttt tatttttaat attcatcata atctcaaata cctaatcctt tcgaaaattt
      901 aacattttgt tccttcgttt ccaattccta attaacattc tatcttacat caaaataaat
      961 tgcaaccttt tttgtaatct ttttgtaatc tttttgtaat cttttgtttc tttttagtat
     1021 atatttgttt ctttttgctc cccctggtta caaattagaa atatattcta cttttctcta
     1081 gttgttgcaa tttctttgat attagcatct tctggtttaa tataagttgg cactaattcc
     1141 ctcataatac catctatttt ttcaacctct tcattgttag taatttctct taaaagtttt
     1201 aagtattttg ttattttttc tctatcaaca tctattggtc tgccaataaa aatcttttta
     1261 tgctctgtat ccaacagacc ttcttctgac ataagtagtt cttcatacaa cttttctcct
     1321 ggtctaagac cagaaaattc aatttttata tcaacatctg gctcaaatcc actgaattta
     1381 attaagtttt ttgctaaatc caatattctt acaggttctc ccatatcaag cacaaatatt
     1441 tctccacctt tagccatagc cccagcttgt ataactaagc ctactgcttc tggtattgtc
     1501 ataaaatatc taatgatatc tggatgagta actgtcactg gtccgccatc ttcaatttgc
     1561 ttcttaaaaa gtggaattac actaccatta ctaccaagca catttccaaa tctaacagca
     1621 acaaattcag tctggctttc agcattcata gtctgtataa tcatttcagc agctctttta
     1681 gtagctccca ttatgttagt agggtttaca gccttatctg tagatataag gacaaatttc
     1741 ttagctctat attttgaaga caagccagct atgtttaaag ttccaaatat attattcttt
     1801 atagcttcgc cagggctcga ttccataaga ggaacatgtt tatgtgctgc tgcatgaaat
     1861 accacttctg gtttatattt attaaatatt tcatccattc ttttttcttc tcttattgat
     1921 gctattactg tctttaaatc taatttattt ttatattttc taagtaattc ttgttgtatt
     1981 gagtaagcat tattttcata attatcaact ataataagct ctttaggatt aaatcctgca
     2041 atctgtctgc aaagctctga accaattgag cctcctcctc cagttattag gattactttt
     2101 ccctcaatgt aattacttat ctctcttaaa tttgttttta taggctctct acccaacaag
     2161 tcttctatct ctacttctct aatctgtttt atatcaactt ttccatctat aatttcgtaa
     2221 attcctggta ttgtctttat tttacagtta gtgtttttac atatatcaat aatctctttt
     2281 tttctacgtt tctctatgtt tgctattgaa aagattatct catctacatt gtacttttct
     2341 accacagctt ttatatccga agttgttcct acaattttca cattatggat tattcttcca
     2401 attttatctt tatcatcatc aattatagcc actgctactt ttttaagttc tgaatttctt
     2461 tttagttcct gtataaccat ttctcctgca tctcctgcac caattataag gatatttgaa
     2521 acctttcctc tagaatatgt cttgttcatg actattctta tagctctata agctattctt
     2581 gcgccacttg taaacattat aataaatatt gtatttaata cataaaacat tataggataa
     2641 tcaaacccta ataatttatt tattatataa atcggaatta aataagctaa agttgccaca
     2701 aaaatagata taatttcttc ttctcctgca tatctccata atgtgttgta gcacttaaac
     2761 aatctaaatg atactatatg aagtactata tatatacaca ctgaaattat tatatcatta
     2821 gtggtaaatg catttaaatt acctgtcaag cttaaagaca ttcgaaacgc tactagtacg
     2881 ctacaaatat ctagaattaa tagtgttaaa tgtctaaatt tatatttttt aaaaagccta
     2941 ttaaaaaagc tttctttctc taaatctata ctttctcgca taactagaag ccctccttta
     3001 aacagcaaaa gctacgcacc taagcatagc caacaacatc tatcttttgc tgtatttttt
     3061 cattttattt tttatattta tcaaatatag aatcataaat tttttctata tcattaaagt
     3121 ttaaattaac ctcataattt tctctaacaa attgacgccc tttcttaccc atattaagtc
     3181 taagttcatc attttccatt agttttatta tagcattttt tatttcttct ggttgttttg
     3241 gttctacaat taagccagtt tcattttccc atacagattc tggatgtcct cctacatttg
     3301 taactataac aggtacttca catgcttctg attctatagc tgccactcca aatccctctc
     3361 ttaaagaagg aaatacagtt acatcaaaag aattaaaagt cttgcttaca ttttctggag
     3421 atattcttcc taaaaactga acatcatttt ctatacccaa ttctttagct aaatttatta
     3481 ggttgtccat ctgacttcca gagccaccta ttttaagaat tattttctta tctttatatt
     3541 catctttaag catcttaaat gcttgtatta aatattctat tccatatttt ttttctaaac
     3601 tctttataat tcctattaca aaagcgtctt ctttttctac ttccataggc ttaaacctat
     3661 ccatatctac tccaaaaaag gttacatcta catgtttagc tgtatattta ttagcctctt
     3721 tagccatatc tctactattt gaaaagatat agtctgcttt tctaaagtta tgttttataa
     3781 tttgtttttg tataaaacca ttattaggga aatcataaat atcagtaccc caaacagaaa
     3841 taatatatgg atgataccca agtagacttc ctataaatcc ataactactt gcatactgtg
     3901 catgcaatat atctggcttt atttcattta ctagcttctt tatctgcttt attgaaccta
     3961 agtatcccgt ttttttagaa atattttcat tttttaattc ttctacatta gaaccaaaat
     4021 tataaatttt tattccttcc atgtgacctc catttaaaga gattacatga atgtcatatc
     4081 ctttattttt aaaaaaacta caccatttta aagtatgagg atttgaccca tcagctaaat
     4141 aacaaacagt cttcataata tttaccttcc tatcgaataa tactcaattc ctaactcttc
     4201 catatcttta attttataac aatttcttcc atcaaggact aaaggatttt tcatcttttc
     4261 cttatactta tgtatatcat aatttaatac atctttccat tctgtcatta taaaacatat
     4321 ctctgcatct tctatcgcat catctacaga atcagcataa agtatagagt caccaaatat
     4381 tttcttagta ttatttacac caattgggtc gtatacagta actcttgccc ctctttcaag
     4441 caaatatttt atatttggta tagatggtgc ttctcttata tcatcagtgt ctggtttaaa
     4501 agtcaaacct aaaacagcaa ctttcatatc ttgaaagctc tctactaact tagatgcctt
     4561 tctaactaat ttaagttttt gactttcatt aacttctatt gcagctttta ctgtttttaa
     4621 ttcataacca ttatgttcag ataaccagtg aagagctttt gtgtcttttg gaaagcaaga
     4681 accgccatac cctattcccg cctttaaaaa cttacttcct attctatcat cataactcat
     4741 tcctaaagct acatcttcta tgttagctcc tgctatttca caaagatttg caatatcatt
     4801 cataaatgat attttaagag ctaagaagtt atttgatgcg tattttatca tctcagcact
     4861 atttctattt gtaactatta taggttgatt aaaaggctta tagatttctt ctaatatttc
     4921 tctagcccat tcactttcta cacctataac gattcttgaa gcatatagag tatcttttac
     4981 tgctgttcct tgtgataaaa actctggatt agatgcaagc tctactttta cattattttt
     5041 taggtttcta tttatataat cttctatctc ttcatttgta ccaattggaa ctgtagattt
     5101 tattactact agagtatctt ttgtcacagt ttcagctatt tgctttgcaa ctgcctttat
     5161 ataatctagg ttggctgagc catcacttct ctcaggtgta cctacagcta ttaatattac
     5221 atctacatct tcataagctt ttttatagtc tgtagtgtaa tctattcttc ctctgttgta
     5281 atttttaacc ataagttctt ctaggccttc ttcatatata ggtgctattc caagtttcat
     5341 tgatttaact ttttgttcat ctacatcaac acagactaca ttttcattgc ctacctctgc
     5401 tagacaaaca cctgtaacca aacctacata tcccgtccca gctatagcta ttttcatttt
     5461 tttacctcca agtatataaa acttatactc tttttttaat ggcattaaaa acatagaaac
     5521 aaaatacata tagacttttt ggtaaactta aattttcaac atttctataa gtattccaag
     5581 ttcgttttaa tgcttttatc ttgttacttg atatagaatt tccaactaat ctgtaattca
     5641 ctaaatcctc ctgaatccca tatgcatatt tttccttttt aagtaactgt aaccaggtcg
     5701 cagtatcttg tcctcttctt accaaaggca tgctgaactc tcctacaatc tccttatcaa
     5761 tgaccactgt agaacatcct attatagtat ttctaagtaa tccttcatag tcaattttct
     5821 taggagctct tgcaacctta ttcgtttttg acccatcttg tctcatatat ctataagatg
     5881 taaaagaaaa acctacattt tcttttaaca tatattctat ctgaatctct aattttctac
     5941 tatcccataa atcatcacta tctacaaatg caataaatcg accttccgca ttcttaatac
     6001 caacatttct agatactgca gctccagaat ttttttcaag ctttatatat tttattctag
     6061 catcttcttt tcgaaaactt tttataattt cacttgaatt gtccttagaa caatcatcca
     6121 ctaataaaag ctgccaattt ttataagttt gattttggat tgactttatc gtctctgata
     6181 aaaactcttc tgaattataa acaggagtaa ttatcgatac taaaggctca ttcatatttt
     6241 tactctcctt atatagcaac tagcagtact ataccaacaa gtttacttag atactaattt
     6301 tctttgtcca atatatttgt aatatcctaa tgggaataat gctaaataca acaatttatc
     6361 agatactttt cttaattcac caaaataatg actatcagac aacaatgagt atctggctaa
     6421 atttatgtaa ttttttatcc acatatatgg atatctactt aatggatatg tattttgatt
     6481 tattaaatat aaaaactcta atgctctacc tcttaatgca tttttactta atatattctt
     6541 agtcaaacca tcatccaaat aatctcttat tatcataggt ttatttacac atataacttt
     6601 atagtattta gacatttcat tccatactac actttctggt aaaaatctca cttcttttct
     6661 ttctggaaaa ggaaatttta taagctcatc agttacaaat actatgcttt tatctccctt
     6721 aacgccatac ttaaaatata ggtctgcaaa gtgacatatt ttcttatctt caggcatttc
     6781 tgtccctatc aaatctccac ttggatatac acaaagtcca acaactccac taaatccttc
     6841 tttattctct aaagaatccc actcatcatt agcaatttca agagaatctt taattaaacc
     6901 atcatcacta tctacaatga aaaatagctc tccatctgca ttttttacac ctagatttat
     6961 tgcagtatgt tttcctccat tttctttgta tatatatctt atatttaatt tattttctga
     7021 tataaatttt tcaactaact ctttagtact gtcgctactt ccatcatcaa caatcaacca
     7081 ttcaaaatca ttgaaatcat aagtttgagc ttttaaatca ttgtacaaat tttcaagtaa
     7141 atgagctctg ttatacgtcg gagtaaatac agtaaattta tatttataca ttaattaaat
     7201 cttcctctct gtctaacttt ttttgattcc gtttattcca taattaataa agtagtatat
     7261 agctttgtat aaaggtattt tttctatatt tctatatata ttccaagtcc acatagcagc
     7321 ttttatttta ttaccactta gggaattgct agattttcta tataatgcta gaacttcatt
     7381 tattccatga gcaatatgcc cttttttcaa tatcgataac cataaaacat agtcttcatg
     7441 tcttacacca ctcattctta tttcaaaatc caatttagac ttatcaatca caacagtaag
     7501 acatcctaag atatttcctt ttaataatct tttataatct acattaggtg gaacttttat
     7561 tactttgttt aattttttat cattttcatc catcagttca tatgaagtaa atgaaattac
     7621 atagtcattt tctaacataa aatttacttg tttttctaac ttactgctat tccattggtc
     7681 atcactatct aaaaaagcta taaattgtcc tgttgcctta cttagtgcta aatttctagc
     7741 attagagaca cccttattag tctcagtctt tatacatttt attctactat cctgttgcat
     7801 ataagatttg actatattag gactattatc tgttgagcaa tcatcaataa ttaacatttc
     7861 ccattcttga taagtttggt ttaatactga ttttatggtt gcttcaataa atttttcaga
     7921 attatacatg ggagtaatta tagatactaa attcttcttc atattacctt cctcatcatt
     7981 tataaaatat ttatttaatt ttacttaata cacaatcata tatcctcttg cagttcttat
     8041 tatcattata tctgaaaaac ttatttcttt catttttttg gttatccata acctcaaaat
     8101 tattttcaat aatatcaata agtttattca cggcttcatc attattataa gccaaagacc
     8161 caaacatttc actttttaaa tctatatatg aatcaacttt ttccatatat tcatccaaat
     8221 cgaattggta aaacaagata ggtctattca tatacaaaaa gtctatggct acacttgaat
     8281 aatcagttat atttgcagct gattttctga tttcatttgc tagagttacc cctttatcta
     8341 aaaacacaat tcgttttcct gttatatttt cttttatatc atctataaac tcatgcataa
     8401 gatgatgcat ataaattttt acatttatat ctttctcatc taatatttta tttaatttgt
     8461 catcatttaa aaaattcatt atatttttaa aataatctgt atccgtaaat tttcctgaac
     8521 taaattttat ccaatctctc catgtaggag tatataatat atctcgagtt tgtgcaactt
     8581 catttgttgg taaattgtca tatcttgcat tcccaattat ataaactgag tcatctaaca
     8641 ttccccaatc atttaccata acatttcttt caaattcaga acttgctgta actatattat
     8701 agcttttata taggttatct ataatatttt tgaacttttt gttcataggt tttctttttt
     8761 taaagcttat agtcccatga tttaaaaaca cttttatcgt ttttttatga aaataattaa
     8821 gaacaggtac agcaaaattg tatggtgcta tatctgctga tggtgcatgt gaaaatacta
     8881 tagcttttga attgtaataa tacagatagt tttctacact tcctcttatt aattttttgc
     8941 ctggaatttt atcaaatact ttactgtctt tatttactac ccaataagtt tctacatcat
     9001 tatgttcttt tagcatatac tcataaaaaa actttgaatt atcattgtat atatcccctg
     9061 aatgtccacc tataagccag atatctctac cattaaactt atgcttatta aatggatata
     9121 taaaaaaagc caaaagcata tttattaaaa atttaacctt gtccataaac accatcctca
     9181 ttataacttg tactgtctaa atccattaat tcttcctttt atagtggcac tatgataatc
     9241 tctattggaa ctcaatataa gaccccctat atttcttaca agttttgata ccataacttt
     9301 tgcaaacgta taattttttc tatagactat ttcctttttg caaagcgctc caaagcctct
     9361 tccataatta taatcttttt gatacttttc tttagattta gaatgttttt ttgcaggatg
     9421 gtaaattata tcatttccaa agtattttcc tttaaaacct aaactcaata agtttaaaac
     9481 ataatctatc tcttcaccag caccaaactc tccaccaaca cccaattttt catcaaatct
     9541 tgtataatct ttaccttcaa aatcaataaa aaaagttatg gatgttatag tatccaaaac
     9601 gttggaactt gtgatatcac aagttccatc atacattttc ttaaatgcat caactttatt
     9661 tgaatccata gttttgcaac tgtatatttt ataatcttca tttttattaa agaaatttat
     9721 aactttttct aatgtatcat tttcgtagac acaatcatca tcagggaaac ctataatttg
     9781 ccctacagca ttatctatgc ctacatttct gttataactc aatccttttt tagagctttt
     9841 tatatacttt atatctaact tatcaatgta tttatcaact atttctttaa ctttgctatt
     9901 atcattttgg tctactacta taagttcaaa gtttttgtaa gtttgatttt ctaagctatc
     9961 cataagtaag tatatatcat catatctatt taatgttggc attattaatg atattaacat
    10021 tactacctcc tacttttcat cttccacaaa tctatttccc ttttatcatt taatatcact
    10081 tcttttatta gtaatactac tgcaaaacta aatgaagctt ctaataataa attttctgca
    10141 aaagagtata gtaaaaatat aaataaagct actaaatatg cttttttatc tttttctata
    10201 aagctttcta ttcctttata catcaaatac ataaataata tacaagcaac tactcctagt
    10261 acactaatta tatatacata agaactatct aatggattag ttgcttttat agcaggacta
    10321 tatgaatttc caaaaggttt tagaaaattc cccttttcag ccaagtatac atgccagaac
    10381 ttaggtctac ttgctaataa tctatttaat aaagtgcttt tatcaaatat tgtaccaata
    10441 attacactaa atagagtaag tattattggt aatgtcttta ggagcattgg cagtcctttt
    10501 atttttttta tatctatcca tcttattatt tctacaaata ttaatcctgc taatatagtg
    10561 aaaaatccag ttctactata tgtggtttga taaacaaaaa atgcagaacc aaata
//
LOCUS       DAEPLF010000117        10580 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEPLF010000117 DAEPLF010000000
VERSION     DAEPLF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10580
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.117"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..1123)
                     /locus_tag="KR314_002429"
     CDS             complement(<1..1123)
                     /locus_tag="KR314_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0328806.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELTILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEIYFPVKAILLTIVS
                     FMILFLITGPMSLKLLRKKSILELLVGTKKPKKEIKSSRLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKE"
     gene            complement(1123..1869)
                     /locus_tag="KR314_002430"
     CDS             complement(1123..1869)
                     /locus_tag="KR314_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328807.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PTILLADEPTGNLDSKSTDDVLKLFTRINKEQNVTTLMVTHEVYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            complement(2016..4235)
                     /locus_tag="KR314_002431"
     CDS             complement(2016..4235)
                     /locus_tag="KR314_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0328808.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLRPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            complement(4601..5995)
                     /gene="gltA"
                     /locus_tag="KR314_002432"
     CDS             complement(4601..5995)
                     /gene="gltA"
                     /locus_tag="KR314_002432"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBH0328809.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLNINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKTKVNA"
     gene            complement(5995..6888)
                     /locus_tag="KR314_002433"
     CDS             complement(5995..6888)
                     /locus_tag="KR314_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBH0328810.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     ILKLEEGDSHHHSNCGCGGNK"
     gene            7514..8365
                     /locus_tag="KR314_002434"
     CDS             7514..8365
                     /locus_tag="KR314_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBH0328811.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(8498..10111)
                     /gene="ggt"
                     /locus_tag="KR314_002435"
     CDS             complement(8498..10111)
                     /gene="ggt"
                     /locus_tag="KR314_002435"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBH0328812.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(10336..>10580)
                     /locus_tag="KR314_002436"
     CDS             complement(10336..>10580)
                     /locus_tag="KR314_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0328813.1"
                     /translation="IKLGANNKDIEKIISKQLKIIFLMPLVVGTVHNLFAMSIAQKFI
                     PRSLLVPIIITLVIYFIGYFIYYFLTLKYALNMIKE"
BASE COUNT         3504 a         1825 c         1127 g         4124 t
ORIGIN      
        1 attcctttga taatttgtta taattcttta cacttagtaa gacttcccta ttatatttaa
       61 gtacaggaaa acttaactcc ttataatcat atcctttgat tgcttctcta atttgttgta
      121 gttcttcata ttctttttca ttattatgct ctgaaaaata tgagtaaacc attgggaaat
      181 tatttttaga agcttctcct tgagaactta caagtgttga agttgttgta aatgctacta
      241 aagtagccga taaaagtatt gttattaaaa ataacaatcg tgtattatcc tttaatttat
      301 atatcaaatt acttataagt aacatattta ctttgttctt ataaaatttt tctttatttt
      361 ttaaatactt taatatcaaa atagtaaatt gagaaaaaaa taaaaatatc cccaaagtaa
      421 ttaatagtgc tgttgtatac atacctccat ttatttttaa cttattgcca aatagtatca
      481 tagcatatcc taatataaga catattacac caattattcc aaatagcctt gaagatttta
      541 tctctttttt aggctttttt gtacctacta aaagctctaa aatactcttt tttctcaata
      601 attttaagct cattggtcct gttatcaaaa ataaaatcat aaatgataca atagtcaata
      661 gtattgcttt tactggaaaa taaatttcag ttaaattcat ataaaacagt ttgcttaaat
      721 acaataaaaa caattttgaa attgataaac caagtaatat tcctaacatc actgctaaaa
      781 atcctattat caaattttca attaatatca attttttcag ttgtctgttg ctcattccaa
      841 ttatcatcaa tactccaaaa tctttcaatc tatatttcaa gaaatttcct aaagagtaca
      901 atataaaaag tagactaaaa cctaatattg ttaattctga agtcataaga gccataaatg
      961 cagtagaacc actctgcatt ccagattttg ttataatcgg atggaacata gacactgcaa
     1021 aaacaaaaaa tatcattata gaaaaaacag aacttaaaaa atatgctaaa tatgccctga
     1081 aatttctaac tatattatta taagcaaact ttctaaaatc cactatctta cacctcctat
     1141 ttgagacaaa acagctagta tatcagaata gaaagaatta ctgctttcag atttttttat
     1201 ttctgtatat atacaaccat cttttataaa aattactcta tctgaataac ttgcactata
     1261 tacttcatga gttaccataa gtgttgttac attttgttct ttgtttatcc tagtaaataa
     1321 tttcaaaaca tcatcagtag attttgaatc aagatttcca gttggctcat ctgcaagaag
     1381 tatggtaggt ttatttataa tagctctagc aatagcacac ctttgagctt gacctcctga
     1441 aatttcatag gtttttctat ctaaaatttt atctattcct aaaaatttag atatactttt
     1501 agtttgtata ttcatatctt tgatacttgc cccctctagt gtaagtggca acataatatt
     1561 ttcgccaaca gttaaagtgt caatcaaatt aaaatcctga aatacaaaac caagttgttt
     1621 ccttctaaat tcagcaagtt tttctccttt caatttactt acatcatatc catctaatac
     1681 aacctcacct tcactctgtc tatcaactgt agatattata tttaaaagag tagatttacc
     1741 acttccagat ggtcccataa cagacacaaa ttctcccttt tctatattta gactaacctt
     1801 atctaatgct ttaaatggaa tatttccgca atatgttttt gatatatttc taatactcaa
     1861 tatgttcatt ttatattctc cataattttt tatttttact acaataccat aatatcgtga
     1921 atatactact ttaaccataa aaatacccta acaattaact tacatatttg taagtaagtt
     1981 gttagggtat attatagtct aataattatt aatgattaat caaatttaaa gtttaagtgt
     2041 ttttcaaatt catctaatac cataggttta gcaaataagt atccttgaac cttatcacaa
     2101 cctatctctt tcaagaactc tacttgttca taagtttcta cgccctcaca gattacttta
     2161 atatcaatcg cttttgccat ttccacaatc ttagaaataa taatttttcc acgttttgta
     2221 tctgttgttt cgattaaaaa tcctctatct aattttaaaa tatcaaaaga aaggtctttt
     2281 aacagattta aagatgaata tcccgaacca aaatcatcca ttgatataag gaatcctatc
     2341 tctttaatct ttttcattat atcaatcaat atatttaaat tatcaaacac aacactttct
     2401 gttagttcaa gttcaataaa ctcaggagat atattgtatt tacttatcaa atcagttata
     2461 ttttctataa aattatccct atataaatga actctagaca tatttacaga gattggtaca
     2521 ggagtatacc ctttatttat ccacttgttt aatgttttgc atacttcttc aaacacgaac
     2581 atatcaatat ttacaataaa accattcttt tcaaataaag gtacaaaatc tataggtgaa
     2641 ataaacccaa attctggact attccatctt atcaaggctt ctgctccttc aatttcattt
     2701 acatcatcta aactatactt aggttgataa tatactataa attcaccttt ttctaaagct
     2761 ttattcatat tatcttccaa tcgtttttcc ttgtacaatc gatttcttgt atcttcatta
     2821 aaaaacgaat atgaatgttc atatttttcc tttttacttt ttgctgcaat atttgctcta
     2881 tcaattaaca ggtcaatctt tttaatatca tcctttttag taatcttata tattccgctt
     2941 gacataacaa ttttatactt agatgagtta gtagtattta agttacaaat ttctttattt
     3001 attttagata atctatttaa aagttcatct tcagtattac aagcaatcaa caatataaaa
     3061 tggtcattat ccatcctagc acaaatttca tatttatttg tattattatt taaaacctgt
     3121 gatatatgaa ttaatatctt attcccttca gcatatccaa acatctcatt tatatattta
     3181 aatttattga catccaattg tactaagtaa aaattatctt gataataatt ctttaaaata
     3241 tacatagaat cttttctaaa cttttcttta ttttttattc cacttaaaga atcataataa
     3301 gccaattttt taagttgttc tattaattta gcattactta atttccacaa tatcataaat
     3361 attactatat aaaaatacaa ctttatcata aaattaacta ttagattttt aaagttctta
     3421 aatatattac attttgatac taaagtccaa ttagtccact ttatgttcgt atattttaca
     3481 ttatataatc tccaattatc ccaagataaa aaatcacctt gacatttttt atttatattt
     3541 tttaagattt cacttttagt ggttcctata atattccctt ctaaatcaag catcttactt
     3601 ttatcattta catatttttt gtttggatga gaaataatag tataatcttt atctatcatt
     3661 agaaatttaa tagaatcatc aaaattgctt ctacttatta aattatttac atcttgaaaa
     3721 taaaaagaag caaaaatggt cccaatcatc tcattatttt ttacaattgg tacatatatg
     3781 ataaatactt tgtcacctgt tacattactg acttcaatat ctgaaactac tacttgcttt
     3841 gttttaattg ctttctcaaa actaggctta tcttttattg agttaacatt ttccctatat
     3901 gtacttgaag catttccttt tgtatctgtt ataccaatca taaaaagatt atattccttt
     3961 tgatatggcc ttaataattt tcccttttca attagtgatg tttcaccatt agaaaatcta
     4021 tcatcttgac taagagcttt taataaagat atgtttactt ctaaatagtt ttcaagttct
     4081 ctttttgatg cctcaatcat atttctagaa gtaatttcca tagagttact tgcatatatg
     4141 atggtacttg ttaaatcaat caaaactaat agtattaata agattaatat atgcttaaaa
     4201 actagcccat caaatacttt ttctcttttt ttcatattaa aataccatta attacacaat
     4261 ttatttttct attatttaat gttagatttg acaaatctga ttttattagc cacacattat
     4321 tcactcctta caagaccttt attgtataac tattttatca tagttagaca aagttatgtt
     4381 acttagtatc aaatattaca ataataactg ataaattatt aaaccatcta aaaaagtgcc
     4441 tacatcaaaa tagagattgt actaagactt aaaagttaga ctttctacag gaatgaaatt
     4501 ttcattgttg aaatgattaa aacatatttg attttgtaca aaaaaagtgc ctcatgaact
     4561 aaaaagttca ttttgagaca ccctctcttt aatttacaaa ttaagcatta acttttgttt
     4621 ttaaatattc atctattgat gctgctgctg ttttaccagc acccattgca gatattactg
     4681 ttgctgctcc agtaacagca tctccacctg caaatacacc ctctttagaa gtctgaccat
     4741 tttcatctgt tattaagcat ctcttcttat ttatattaag actctttgtt gtagaagata
     4801 ttagtggatt tgggcttgtt ccaagtgaca ttataactgt gtctacatca agtacaaatt
     4861 cagaaccttc tatttctata ggtcttcttc ttccagaatc atctggctct cctaattcca
     4921 ttcttataca aaccatacct ttaacatatc cattttcatc tactaaaatt tccttaggat
     4981 ttgttaatgt attaaatata atgccttctt cttttgcatg atgaacttct tcagctcttg
     5041 ctggaagctc tttttcactt cttctataaa ctatatatga ttctgaacct aatcttaatg
     5101 cagttctagc agcgtccata gcaacatttc caccacctac tacagcaact tttttaccag
     5161 aactaatagg tgtatcatag tcatctctat aagccttcat taagtttact ctagttaaaa
     5221 actcatttgc agaaaacact ccatttgcat tttcccctgg tatattcata aacattggaa
     5281 gaccagctcc tgaacctatg aatatagctt caaatccttc atcttctata agttcatcta
     5341 ctgtaattgt tttaccaaca attacattag tttctatttt tacacctaat tttcttatat
     5401 tgtctatttc aggttgaaca acagcttgtt ttggaagtct aaactcaggt attccgtaag
     5461 ttaaaactcc acctggctca tgcattgctt caaagattgt tacatcatag ccttttttag
     5521 ctaaatcacc agcacaagct agacctgctg gaccacttcc tattactgct actttttgat
     5581 ttttcttagg ctctgtatca gataaattta tgtcattttc ttttgaccaa tctgctacaa
     5641 atctttctaa tttacctatt gatacagcat ctgattttat tccaagtata catacacctt
     5701 cacattgact ttcttgtggg catactctac cacaaacagc tggtaatgaa ctgctcttag
     5761 ctattacttt agcagcacct tctatatcat cttctttaat ttttgttata aaccctggta
     5821 tgtcgattcc tactggacat ccacccacac attttggttt tttacaagct aaacatctat
     5881 tagcttctgc catagcttct tctttattat accctaagca tacttcatca aaatttgttg
     5941 ctctcacagc aggttcttgt tctctaactg gtacttttac ttttttagca tccattattt
     6001 gttacctcca catccacaat tactatgatg atgactatcg ccttcttcaa gttttagaat
     6061 agctcttccc tcttgagtct tgtacataga ctgtcttctc attgcttgat caaaatcaac
     6121 taaatgacca tcaaattcag gaccatctac acatgcaaac ttagtttcat ttccaacact
     6181 aactctacaa ccaccacaca tacctgttcc atctatcatt attgtattta aacttactat
     6241 agtaggtata tttagttctt tagttaattg gcacataaat ttcatcatta tcattggtcc
     6301 aattactatt gcttggtcat attttttacc ttgattatca actaaatctt ttaataggtc
     6361 tgtaactctt ccattaaatc catatgtacc atcatctgtt gatacatata catttttagc
     6421 tacttttttc atatcatctt ctaatataat taattcttta gttcttgcac caattattac
     6481 atctacatct aatccatgct ctttaaacca tttaacttgt ggataaactg gagcagaacc
     6541 aactccacca gctacaaata atatcttttt atttcttaat tcttctatat cttcatgtat
     6601 aaattcactt gcttgtccta atggccctac aaaatccatt acaaaatcgt tttcttcaaa
     6661 cattgctaac tttttagttg aagcaccaac tgtttggaat acaatagtta ctgttccttt
     6721 ttctctatca tagtcagcta ttgtaagagg aatcctctct cccttttcat catttttaat
     6781 tattataaat tgacctggtt gagaagattt tgcaactcta ggtgcctcta tttccattaa
     6841 ataaatacta tctgttaatt gtctcttact aactatttta ttactcatct cgctattctc
     6901 cattctaaca aatatatttt tcccacttta aatttaatta aaaacataaa cacacaaaac
     6961 tataactctt tataatataa tattagttct tggttgattg ttttgtattg gacatccccc
     7021 ctatagtaat tataactgcc caaactacaa atagttgata ttaacaaatt tcttgtaaat
     7081 gccaataaac actaagtcta tatcatacgt ctaatttgtt atagatttaa cctaatacac
     7141 acttaaagta tactatatta tgccaaattt gtaacaaaaa ttttataaat acataataaa
     7201 atttttttat tcaattattt atttaataaa ttaacttagg ttttcataat aacactaatt
     7261 tctttaaaaa ctaaacgtgt ataaggcttt aatctattgc aataccagct ttcatattat
     7321 ataaaatata ctcttatttt attatatatc taaaattttc aatattcatt aattatttga
     7381 atttttatat aaaattttta caatttctaa agatttattt acttatgtaa taaattataa
     7441 taatattagt ttataaagtt aataattaat ataaattaaa ataaagataa tataaacata
     7501 ggaggtacat tctttgaaaa acattatttc agcaaaagaa cttatatcaa aactagaaaa
     7561 aaatgataat ctagttgtaa tagattgcag atttgatctt atcaatagaa cttatggtat
     7621 tgattcttat aaaaaaggac atatcaaagg cagttttatc ttagatatag acaaggattt
     7681 atcatctcct acaaaagaac atggtggtaa aaatccttta caagaccctt taatactaaa
     7741 agaaaaatta gaaaaaatgg gtgttgataa tgacactacg attgtaactt atgatgatgg
     7801 agatttaaat ggagcttgca gattattttt ccaattaaaa catttggggc ttaaaaatgt
     7861 ctatgtacta gatgggggaa taacatcttt cgtaaaggaa ggtggagaat tggaagagaa
     7921 agtgaatata ccaagttgta caggtaagga aattagacca aatataaaca atgatttagt
     7981 tgttccaatg gaatatgtta agtctaaatt atacaaaaaa gatactgtaa taatagattg
     8041 ccgtgctaat gagagatacc agggtttagt tgaacctgct tactcaaaag ctggtcatat
     8101 accaagtgca aaaaattatt tttgtaagga tttaataaaa agtgactttg aaaatggctc
     8161 acttaaagat atagaatttt taaaaaaatt ctttaaagat ttaaataatt atgatgaagt
     8221 aattttatct tgtggttcag ggatatctgc ttgtgttaac agtcttgctc ttagagaact
     8281 ggatatacct cacaagattt atattggtag ctttagtgac tggattagtt atgatgacaa
     8341 tgaaatcaaa actggtcaag aataaggctc ataatctata taaaatcagc atatgatagc
     8401 ttaaattttg taaaggcgtc agtcattgta gactttcgcc tttactaatt atttcatata
     8461 ctctttaaat ttattatata tattttagtt gataatatta ataaactgca atatgaccat
     8521 cacatcttga ttccgtccca caaatataac tttcctgttc tgttttaaat attatttgac
     8581 ctcgtcccat gtcgacagta tcatgaatta cttttatttc atgacccttt tcaattaatg
     8641 aatctattat atgctttggc atctctggtt caacttctat ttcctttcct tttatccact
     8701 gccatcttgg agcatctagc gcctcttgtg gatttaatcc aaaatcaatc atatttgtta
     8761 acacttgaat atgtccttgt ggttgcataa aagctcccat tacaccaaat ggacctattg
     8821 ctttatcttc tttacctaaa aatccaggta ttatagtatg ataaggtttt ttaaatggtt
     8881 ttactacatt gtggtgttta gggtctaaat taaaattatt tcctctacta tgtaaagcaa
     8941 tcccagttct tggtatcaca attcctgaac caaaatttat gtaattgctc tgtatatatg
     9001 aaaccatatt tccatcctta tctgcagtac aaaggtatac tgtaccacca caatatggct
     9061 ctcctgcagt tggatacaaa gcctctttat tgtcaattaa taaacttctt tttttggcat
     9121 actcttggct tagtaattcc tgaatcttta ccttcatatg ctctatatca gttacatatg
     9181 ttttagaatc tgcaaaagct aattttaaac tctctataat tttatgtatg tcttctacat
     9241 tttctatatt tccttctaat tccaattctt ttaatatgtt aagtgccatt aaaactgtta
     9301 ttccatgacc atttggtggg atttcaaata ttttatatcc cttatattct gtacttatag
     9361 gttctaccca tgttggataa aaacactcta agtcactctt tcttatagcc ccattaaatt
     9421 ttttagaata ttcatctatt ttatcagcaa gtcttcctct ataaaagctt tcagcctgag
     9481 tatttgctat ctcttctaat gtacttgcct gttcttcaca tataaatata tctccagcct
     9541 ctggagcttt accatcttta gaaaatgtat caaaccatgg tttgaactct tctccaataa
     9601 gttctttttc atagagttca tatgattttt tccatacctt agctacatta ggagatacta
     9661 cgtatccctc tcttgcatag tttatcgctg gagataagca ttccattaag ctaagtttac
     9721 catatttttt atttaattct gaccaagctg cgggtattcc aggtacagtt actgctccaa
     9781 acccatattt tggcatttct ttcatattat tataattttt taattccata ttttctggag
     9841 caaagccact agaatttaaa ccatacaatc gtttttcttc ctctatccaa acaagggcat
     9901 atgcatctcc tccaatccca ttgcttgtag gttctactac tgctaatgtg gcggctgttg
     9961 caactgctgc atcaacagca tttccaccct tttttagtat ttcaagtcca gcttgagatg
    10021 ctaatggtgt tgatgttgca actgctccat ttttagcata cacgacattt cttattgatg
    10081 agtatttatg ttggtaggca ttaaacttca taaatttcag ctcctcgtca ttttttattt
    10141 gatattctgc ttagatttat tatataacat caagtaatgt aactgataca aaatgatata
    10201 aaatatagac tttttcctaa ctttaatttt atctttaaat aaatatctta aactcaattc
    10261 tagtattttc tatctgcact taaataagtt attttattca aaacatatat ctaattcttt
    10321 tgacttaaaa ataatttatt cttttatcat gtttagagca tattttaaag ttaaaaaata
    10381 atatataaaa tatcctataa aataaatgac tagagttatg ataattggta caagtaatga
    10441 ccttggtata aacttttgtg ctatagacat agcaaataaa ttatgaactg tacctacaac
    10501 taatggcatt aaaaatataa tttttaactg ttttgatatt attttctcaa tatctttatt
    10561 atttgctcca agttttatta
//
LOCUS       DAEPLF010000118        10480 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEPLF010000118 DAEPLF010000000
VERSION     DAEPLF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10480
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.118"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            338..949
                     /locus_tag="KR314_002437"
     CDS             338..949
                     /locus_tag="KR314_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328814.1"
                     /translation="MNIKRINLLLSTVMAAVIISLPLAVTANAMEKTESDNNVKIELS
                     DKEVEELHNYWPEDKIFSDEKIDEILIKTGQFTQKELDKINQEVDDSAKKVNQNSRVK
                     YINGYNKYRTFTKNGKNYLYIYVSGKTLKKIKAGTDLASTFGGFLPKIWVELDVVAIG
                     KIITINMDGINTNYGIVLSYIEDKWNFQGATYTYRYDHWFYQT"
     gene            977..1159
                     /locus_tag="KR314_002438"
     CDS             977..1159
                     /locus_tag="KR314_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328815.1"
                     /translation="MKKRLYKVFLKTIPNLIIVILSLALSFYTNTFDIDLKILLLIPL
                     AYVLLFIYYYIKTKKD"
     gene            1399..1941
                     /locus_tag="KR314_002439"
     CDS             1399..1941
                     /locus_tag="KR314_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0328816.1"
                     /translation="MLIYLAILELEEDKIKFEKVYEKYRQIMFYVANKILKDDHLSED
                     AVHNAFLRIIKNIDKIDEVDSPRTKAFIVIIVERIAIDFYRKRKREKVSDIEEEYKNR
                     EINFSIEDKVCESNLAIALAKLNESYFQVLSLKFQYGFSNKEIANALNLGEENVYKRI
                     QRARKKLKEILEEMEVGNIE"
     gene            1973..2635
                     /locus_tag="KR314_002440"
     CDS             1973..2635
                     /locus_tag="KR314_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HBH0328817.1"
                     /translation="MLRESIPIATKIIIDDLLSDDELNHVFSKGFERKMKKLIKQQKR
                     SSLTNKIIFYSKQTAIVFLIVLASLLTITMSSEALRSRFFRLVIRVYEDLTSFVFSTN
                     GEDNLSLKNKEPEYIPKGFKEVDRVDDFVITYRNDENIEIQYVTDKISSNSIILDTEN
                     AKVKDIFVNGHKAKYIVKGTFLQLFWNDNNFIYLLNVDYQNSGESEFYEDILIKIAKS
                     IK"
     gene            2825..3142
                     /locus_tag="KR314_002441"
     CDS             2825..3142
                     /locus_tag="KR314_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328818.1"
                     /translation="MKSDLYLCKSNKLYRDNEVLEKFMEDLSFLIDDYKFSLETISIL
                     CKINIEKLTNFYAGKGYILYEEMSLIESVLAPFLSAIDIAKHNYDVVIKSRESDKPLI
                     FEN"
     gene            3164..3607
                     /locus_tag="KR314_002442"
     CDS             3164..3607
                     /locus_tag="KR314_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328819.1"
                     /translation="MKRLFALLLTLSLVILPSTLVFANEKAENDSIYAEAPLVFQSNE
                     KAYTHGSGGTAYIGYMSSKHLNWRITSSKGIVILFSGTVEIRNSKNMLVASYPISGGG
                     SSTISGQIDVKYLKKGDYKAILKGTSTTGGGVLVVKSGLVQMFTI"
     gene            3825..4268
                     /locus_tag="KR314_002443"
     CDS             3825..4268
                     /locus_tag="KR314_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328820.1"
                     /translation="MNLRKTVISGMCALVCSSAVAIPAFADEYQDKNIEQYSAVNYID
                     DTMVKMSYIKSGTCNLSISGSTAKVTCFVRGASNVTSTSIIARLQKSNDNGKTWSTIQ
                     TWNASGNISCSLSKSKTIGKGYYRVHSTIKANSESKNIISSTKKY"
     gene            complement(4399..4819)
                     /locus_tag="KR314_002444"
                     /pseudo
     CDS             complement(4399..4819)
                     /locus_tag="KR314_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            complement(4797..6074)
                     /locus_tag="KR314_002445"
                     /pseudo
     CDS             complement(4797..6074)
                     /locus_tag="KR314_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(6097..6639)
                     /locus_tag="KR314_002446"
     CDS             complement(6097..6639)
                     /locus_tag="KR314_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328821.1"
                     /translation="MLLCDPASAGGSKHDYSAFLVGSESENGLLCGRLAELAKINART
                     DFDKYILHMIYLLKVYPDITHVYIEKNTFNGTAANQLELKIKNDDVLYYRDIEIINEH
                     QKKNKDDKISTLIPVLNKGQMIFAEEDKAFIQQILNFTGQKYSLHDDAPDISAEFINR
                     IFNIKVNESITLLDRRNLGL"
     gene            complement(6679..6897)
                     /locus_tag="KR314_002447"
     CDS             complement(6679..6897)
                     /locus_tag="KR314_002447"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328822.1"
                     /translation="MLFDFKNTNHLEDAKEFYWENEKEIKFPILWPSFWNCLDMALSY
                     YENPTSFKQEVQNDVNSIGEKWFKTIRT"
     gene            complement(7093..7836)
                     /locus_tag="KR314_002448"
     CDS             complement(7093..7836)
                     /locus_tag="KR314_002448"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328823.1"
                     /translation="MDNLERNIQLIFSYMAKTFIRNGATIREAEKEANEIIKTNSSNL
                     WGVNGLAYQLGKINLEFFCMYFIQDTYLPKEDNVAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGKKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKKFSDDVKYAA"
     gene            complement(8136..8693)
                     /locus_tag="KR314_002449"
     CDS             complement(8136..8693)
                     /locus_tag="KR314_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0328824.1"
                     /translation="MVVGYMRVSTAEQNEDRQLVTMKKHNVEKMYQEKISAKDTKRSK
                     LNAMLDFVREGDTIIVHDFSRLARSTKDLLEIVELLEKKHVKLISDKENLDTSTPTGK
                     LMLTMLGAIYEFERTNMLERQREGISIAKEQGKYKGRKEVKIDNFGSYYDRYKTRELN
                     KTQLAKELGVSRPTLDRLIKSHESN"
     gene            complement(8888..9076)
                     /locus_tag="KR314_002450"
     CDS             complement(8888..9076)
                     /locus_tag="KR314_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328825.1"
                     /translation="MNNKKKSFLISGTINVILLIIVFALFSYNIIENSTRNMIITVIL
                     IFIFEIIKIKILEKYYNL"
     gene            complement(9087..9662)
                     /locus_tag="KR314_002451"
     CDS             complement(9087..9662)
                     /locus_tag="KR314_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328826.1"
                     /translation="MLKKKIISFLMAGIFVLSSGYNLVYASTFEENEYKQELVNENEF
                     TIINSNENEMIVDKKGNNTYVSTKVTPGSWKNVKYLGQKSRKLSSPYTRERIIQTIQW
                     GLGYLSGGSSFLLGLAESIYREKVYDITKYGGVYTKTYYRRQITDIRNSHANVPVPYR
                     YEYVTYFYSSSNFKKVVYTERVGDGIKLANN"
     gene            complement(9995..>10480)
                     /locus_tag="KR314_002452"
     CDS             complement(9995..>10480)
                     /locus_tag="KR314_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBH0328827.1"
                     /translation="YLINKGYDTFKEFSISYIISKNRTKYYKAILDVENKGYNLTVFI
                     EFMLKSIIQSINEMRDMHDRKSLESILKEELFENDITLSATEEHILKYICSKDNYSMT
                     LENYIKKNKGRYLKAGIKEIELVDQLMEVFNNLEEIEILSREKDVYKVNEKYLNMLEI
                     S"
BASE COUNT         3716 a         1419 c         1297 g         4048 t
ORIGIN      
        1 taaattttta ttcaaagaat acacataaat atttccagac tcacttattg tataagttat
       61 attctatttt gtatgaagtc aattaaaaac tgaactatat taaaattaat tttataaata
      121 acaaatataa ataattacaa aaccttaact gcgttaagtt tgaaatataa taatattaaa
      181 aacacttctc tcaaatcaag tatcttttgt tatattttca taagttttaa aaatataaat
      241 tttttaaaac ttgtgtccaa aaaacaaaat taaatatgtt atatatgtaa agggttaatt
      301 ttaaaacctt aaatttatta tagttagggg gatttaaatg aatatcaaaa gaattaatct
      361 ccttttatca acagtgatgg cagctgtgat aatatcttta ccacttgctg ttacagcaaa
      421 tgcaatggaa aaaacagaat cagataataa tgtgaaaata gagttgtctg acaaagaagt
      481 agaggaatta cataattact ggcctgagga taaaattttt tcagatgaaa agattgatga
      541 gatcttaatt aaaacaggtc aatttactca aaaggaattg gataaaatca atcaagaagt
      601 ggacgattct gctaagaaag taaatcaaaa ttctcgtgtg aaatatataa atgggtataa
      661 caaatacaga acttttacta aaaatggtaa aaactattta tatatttatg taagtggaaa
      721 gacattaaag aaaataaaag ctggaacaga tcttgcatct acttttggag gttttttacc
      781 aaaaatttgg gtagagcttg atgtagtggc aattggaaag ataataacaa ttaacatgga
      841 tggtataaat actaattatg ggattgtttt atcatatata gaggataagt ggaattttca
      901 gggtgcaaca tatacttata gatatgatca ttggttctat caaacttaaa agacatgctt
      961 ttttaggagg aataatatga aaaaaaggct ttataaggtt tttttaaaaa caattccaaa
     1021 cctgataatt gtgatattat cacttgcatt atcattttat actaatacat ttgatattga
     1081 tttaaaaata ttgcttttaa tacctttagc atatgtactt ttgtttatat actattacat
     1141 caaaacaaaa aaagattaat ttttatggaa ttagatatat acgaaaataa ttaatttaaa
     1201 aatttaactt tgagaatcat ttgtagagtt aaggccctta taatcccgaa aatggatttg
     1261 aagggccttt ttactagata tctttaaaaa gcttgttaag catagttata ttctatttaa
     1321 gtagagttaa ttatatattt atgttataat tgaactttaa aatattaatt cagaattttt
     1381 atattggagg aattctaaat gttaatttat ctagctatac ttgaactcga agaagataaa
     1441 ataaagtttg aaaaggtata tgaaaagtat agacaaataa tgttttatgt agccaataaa
     1501 atattaaagg atgatcattt atcagaagat gcagtacata atgctttttt aagaataatt
     1561 aaaaatatag ataaaattga tgaggtagac agtccaagaa cgaaggcttt tatcgttata
     1621 atagtagaaa ggattgctat agacttttac agaaagagaa aaagagaaaa ggtatctgat
     1681 attgaagaag agtataaaaa cagggagata aatttttcta tagaagataa agtatgtgaa
     1741 agtaatttag ctatagcact tgcaaaactt aatgaaagtt actttcaagt gctttcctta
     1801 aagttccagt atggtttttc aaataaagaa atagcaaatg cattaaattt aggggaggaa
     1861 aatgtatata agagaattca gcgtgctaga aaaaaattaa aagaaatatt agaagaaatg
     1921 gaggttggaa atattgagta ataaaaaaga tgattttgat attacggatg atatgcttag
     1981 agaaagtatc ccaatagcga caaaaattat tattgatgac cttctttcag atgatgaatt
     2041 aaatcatgtt ttttcaaaag gttttgagcg aaaaatgaaa aaactcatta agcaacaaaa
     2101 aagaagtagt cttacaaata aaatcatttt ttattcaaaa caaacagcta ttgtattttt
     2161 aatagtttta gctagtttac ttactattac tatgagtagt gaggcattgc gttcacgttt
     2221 ctttagatta gtaataagag tatatgaaga cttaacttca tttgtttttt caactaatgg
     2281 agaagataat cttagtttaa aaaataaaga acctgagtat ataccaaaag gatttaaaga
     2341 agttgataga gttgatgatt ttgttattac atatagaaat gatgaaaata ttgagataca
     2401 atatgtaaca gataaaatta gttcaaattc tattatatta gatacagaaa atgcaaaagt
     2461 taaagatata tttgtaaatg ggcataaagc aaaatatata gtaaaaggaa ctttcttaca
     2521 gttattttgg aatgacaaca actttatata tttattaaat gtagattatc aaaatagtgg
     2581 agaatctgaa ttttatgaag atattttgat aaaaatagcg aaaagtataa aataattttt
     2641 ttaagtgtcc aagggtagcc tcttcattcg ttatacaagt atatatgaaa atgtgaggtg
     2701 atttttatgg ttttaaaatc tacatttacc tgagcgttaa aaatattgtt ataggaaaaa
     2761 ttaatacatt atagttataa catgcttgca ataggaataa aactttaaaa ttgaggtgta
     2821 aaaaatgaaa tcagatttat atttatgtaa aagtaataaa ttgtatagag acaacgaagt
     2881 tctagaaaaa tttatggaag atttaagttt tcttatagac gattataaat tctcgttaga
     2941 aacaatatct attctatgca aaataaatat tgagaaatta actaatttct atgcaggaaa
     3001 aggatatata ctgtatgaag aaatgtctct tattgaaagt gtacttgcac cttttttaag
     3061 tgcaattgat attgctaaac ataattatga tgttgtcatt aaatcgaggg aaagcgataa
     3121 acctttgata ttcgaaaatt aatttaaaac tgggggaata cacatgaaaa gattatttgc
     3181 gttattactt actttaagtt tagttatatt accatcaact ttagtatttg ctaatgaaaa
     3241 agctgaaaat gattcaattt atgctgaggc gccattagtg tttcaaagta atgaaaaagc
     3301 atatacacat ggtagtggag gaactgcata tataggttat atgtcaagta aacatcttaa
     3361 ttggaggata acatcttcaa aaggaatagt tatactattt tcaggaactg ttgagattag
     3421 aaattcaaaa aatatgcttg tagcatcata tccaataagt ggtggaggat cttcaactat
     3481 atctggacaa atagatgtta aatatcttaa aaaaggggat tataaagcta tattgaaagg
     3541 tacaagtaca acaggaggcg gagtcttagt agtaaaatct ggtctagttc agatgtttac
     3601 aatttaatat tataagtaaa ataaaaatca tgaatttatt gggggatagt cacaatacat
     3661 attatttaca gatattcata actattatag ttaaagacat tctattataa agaaaatgta
     3721 taatttaaaa ttataataaa aaaaattatc attagtgtcc aaaaatcact tcttcattcg
     3781 ttatataagt tatataaata gaaaacaaat gaagaggtga ttttatgaat cttagaaaaa
     3841 cagttataag tggtatgtgt gctttagtat gttcatcagc agttgcaata cctgcatttg
     3901 ctgatgaata tcaagataag aatattgaac aatattcagc agtaaactat atagatgata
     3961 ctatggtgaa aatgtcttat attaagtctg gtacttgcaa tttaagtatt tcaggaagta
     4021 cagctaaagt aacatgcttt gttagaggag catcaaatgt tacaagcaca tcaattattg
     4081 ctagattaca gaaaagcaat gataatggga aaacatggtc aacaatccaa acatggaatg
     4141 cttcaggaaa tatttcatgt tctctttcta agtcaaaaac tataggaaaa ggatattaca
     4201 gagttcattc cacaataaaa gctaactctg aaagtaaaaa tataataagt agtacaaaga
     4261 aatattaaaa atagatattg tatttatgag ttgtagtaaa caagaataat agttttttaa
     4321 aagcaaatat aattaatttt aaatagagtg ccaatataaa ataggtgtat aatatttata
     4381 cttttttata ttggcactct attttataaa ataagttatt atagaaaatg aaacataatt
     4441 tatatataaa ttttctgttt gacctggtat ataatctttt atatcacata tcagtatttt
     4501 cccatcatta taaccccaac caatcacagt tgcaggtatc atatcagctt ctccatcata
     4561 aatgattgta tgtttataca tgatttttcc ctcgctatta tctatagttt tattcaaaat
     4621 accttctgct attaatttag caactatgtc tttaatagtc tgtatctgct ttactgtcta
     4681 caaaacatac ttcaattaat atcgctggag cttttgtatg actaagccaa tacaattccc
     4741 ttacgtccga ttttgcacct ctattttcag cagatttata tatatcttca gtcaatttag
     4801 ttataatctt ttgattcatc taataaaatc tctccctctg aaactatttc ctgttctttt
     4861 ttagcctttt ccatttctgc atgaggatta tcaataaaac ttggttgaga taatccggtt
     4921 tcagtagata atttatctcc aagttgactt aatatatttg ctacaactaa atcattatgt
     4981 ggtatcttag gggtaaattt agccttaata tctttataat catagtcaaa accatatttt
     5041 ttattaagcc aaatgcacaa taatttgtac ctattaaata aagtatctgc aatactatca
     5101 ttattctggc tacaaatcca ttctgtactg attaatcttg ctacaactgc taaacttgat
     5161 gtattacttt gcattttttc attgtgattt atatgagatg taattttata tatgttttct
     5221 tttattgaag atagtgtatt ttgaacaaat gtatcattca tgtctttaat aagccattct
     5281 atttttccat ctccattaac ttgaagtatt ccaagttctt tcatccttgg caaatcatct
     5341 tctttaatat tacattcaga gaaggttaaa taagcatttc taaaatcact aatttcatta
     5401 cttatatcac ttaaattagt ttcataagca tcttgtaatc cttttatatc attgaatatt
     5461 gtattttttc ctagttcttc acttctttta caaattgata ttggaacatt accattaaat
     5521 atattattta ctgtaggttt ttctatctct ttaaaatttg aatcaaaatg atatatgtat
     5581 tctttatcat atacatctat ataaatatca tctttaaaat cttttttaaa ttctctaaaa
     5641 aaatagatgg tctctccatc ttcattttca taatggcagc catctgttgg ttttattatt
     5701 cttactttca tttcattgtt ttttacatag taaagttcat agcaaaatcc aaataatagt
     5761 gtatatctca ataaatctga atcgtgcttt ttactccaat ggcaagtata gtattctaaa
     5821 tcatttataa tcttctcatc acctaatcta cttgtatagg tgattttatt tgcaagagaa
     5881 taagcgacct cttcattaat aaactttttt aagaaattag tgtttatctt attattagac
     5941 ctcttggtaa ccattttata gttactaata gcatctgttt cacctttata atacttatac
     6001 atagtgttgt attcttgctt ttttaaactc tagtcttcat atatttctct taataaactt
     6061 aaatctattt ccatttaatg catcacctcc atacaatcaa agacctaaat ttcttctatc
     6121 taatagtgta atactttcat ttactttaat attaaaaatc ctatttataa attctgctga
     6181 tatatctggt gcatcatcat gtagagagta cttttgtcct gtaaaattaa ggatttgctg
     6241 tataaatgcc ttatcctctt ctgcaaaaat catttgacct ttatttaaaa caggtattaa
     6301 agtagagatt ttatcatctt tatttttctt ttggtgctca ttaatgattt ctatatctct
     6361 gtagtaaaga acatcatcat tttttatttt aagttctaat tgattagcag ctgtaccatt
     6421 aaatgtattt ttttctatat aaacatgtgt tatatctgga tacactttta ataaataaat
     6481 catatgcaat atatatttat caaaatcagt tctagcattt attttggcta gttctgccaa
     6541 tctgccacat agtaaaccat tttctgattc acttccaaca agaaaagcac tatagtcatg
     6601 tttagaacca cctgcagatg ctgggtcaca taataacatt gtctttttga atgtatgagt
     6661 ttctatacat tctctagatt aagttctgat tgttttaaac catttctcac ctattgaatt
     6721 tacatcattt tgtacctctt gtttaaaaga tgttgggttt tcgtaataac ttaaagccat
     6781 gtctaaacaa ttccaaaaac ttggccataa tataggaaat ttaatttctt tttcattttc
     6841 ccagtaaaat tctttagcat cttcaagatg atttgtgttt ttgaaatcaa ataacaactc
     6901 tttgaaatta agccataaac cagaattaaa taattcatct acattatcaa ctaaaactcc
     6961 tttttcacat ttaaatttcc aagttggtaa attctttagc ctactataga aacattcttt
     7021 atgttgtaag gttcctaatg ctataaaagt tgttcctctt tttataatct taccatttct
     7081 tattactggt ttttaagcag catattttac atcatcagaa aattttttaa attttttctc
     7141 tctagcatct tctgttctaa cattttcttc tgactgataa tcatcaagta tgataagatc
     7201 tggacgatta ttattgtatt tttttcctct cattggtgat gaagatgata ttgcttcaat
     7261 aaaggtttta ttagttaatt ctaattgagt actattgcaa atatattttc tatcattatc
     7321 atttaagaga actccaaatg cttttttaat atactcattt tcaagtaatg cattttttat
     7381 atcttttacg aatttttctg ctgtagagcc tatatcagag caaattaaag tatatgtctt
     7441 atgtttataa caatgtgacc atacagttgt tgctaaatct ccaaatgcac ttttacctgt
     7501 tcctcttgga taaattcttc ctaattgctg tgaaccatct ccaattatag actcttgtat
     7561 atcttcccat aactcatgat gaactttagc tataggagca gcaacattat cttcttttgg
     7621 taaatatgtg tcttgtataa aatacataca gaaaaattct aaatttattt ttccaagctg
     7681 ataagctagg ccattaactc cccaaagatt gcttgaatta gtttttataa tctcatttgc
     7741 ttctttttca gcttctctta tagttgctcc atttcttata aaggtctttg ccatataact
     7801 aaatattaat tgtatatttc tttctaaatt atccactcgt atttacttcc tcttaattct
     7861 tctatttttt taggttcagt gagtaataca attaataaaa taaacaatgc aaattttttt
     7921 ctaataaaat caccctcttt gaaatatgtc tctggttttt aatttttgct agaaaatttc
     7981 tagaactcat tttctataaa taatactttt ttgaaataga aggatacccc ctatactgaa
     8041 aaacagttat ttataattat tttaaacata attaaagggc ataattctat gaatttgccc
     8101 cactaaaatt aacttaagca tatatattgc tcttcttagt tactctcatg gctctttatt
     8161 aatctatcta atgttggtct acttactccc aattctttag ccaattgagt cttattaagt
     8221 tctctagttt tatatctatc ataataacta ccaaagttgt ctatcttaac ttctttacgt
     8281 cctttatatt taccctgttc tttagctata gagatacctt ctcgttgtct ttctaacatg
     8341 tttgttctct caaattcata tattgcgcca agcattgtaa gcataagttt tcctgtaggt
     8401 gtacttgtat ctaaattttc tttatcacta attaacttta catgtttctt ttctaagagc
     8461 tcaactattt ctaataagtc tttagtactt ctagcaagtc tactaaagtc atgtacaatt
     8521 attgtatctc cttctcttac aaaatctaac attgcgttta gttttgatct tttagtatct
     8581 ttggcactta ttttctcctg atacattttt tctacattat gctttttcat tgtaactaat
     8641 tgtctatctt cattttgttc tgctgttgaa actctcatat aacctactac catctttgat
     8701 acctccacct atgtttactc tataaataat attatagcat aaatgtaaaa ctagagttaa
     8761 agatttgcca tgctttttgt aaattaattg gagttttgat tttattttta catcagaata
     8821 gatagtttgt aatgtaaatt tgtaccagca tactttacaa tatgctctta ggaaagatat
     8881 ttcttgttta tagattataa tatttctcta gtatctttat ttttataatt tcaaaaataa
     8941 aaattaaaat tacagttatt atcatatttc gtgtactatt ttctataata ttataactaa
     9001 acaatgcaaa aacaatgatt aataaaatta cgtttatagt gccagaaatc aagaatgatt
     9061 ttttcttatt attcatatat ttcctcctag ttatttgcta gttttatacc atctcctact
     9121 ctttcagtat atacaacttt tttaaagtta gatgagctat aaaaatatgt aacatattca
     9181 tatctgtatg gaacaggaac attagcatga ctgtttctaa tatcagttat ttgtctccta
     9241 tagtatgttt ttgtatatac gcctccatat ttagtaatgt catatacttt ttctctataa
     9301 atactctcag ctaaacctaa taaaaaagat gaccctccag aaagataacc taatccccat
     9361 tgaatagttt gtataattct ttctcttgta taaggagaac ttaatttgcg tgatttttga
     9421 cctagatatt taacattttt ccaactacca ggtgtaactt ttgttgaaac ataagtgtta
     9481 ttgccttttt tatccacaat catttcattt tcattgctat taataatagt aaattcgttt
     9541 tcattaacaa gttcttgttt atattcattt tcttcaaaag tagatgcata aaccaaatta
     9601 tatccactag ataaaacaaa tattcctgcc attaaaaaac ttattatttt tttctttaac
     9661 ataatatttt actaccttta cttaaatatt tattctataa acactgtcat ataacctgtt
     9721 agttatatta aaaataatct ttttatataa cttcacaatc aaaccttgtc ttatttcata
     9781 atagttttat ttaatatcat ataatttaga cctccttata agattattta tatatataac
     9841 gaataataaa agtaattttg gacaaaataa aaatcttttt attttaagaa tgaaaaattt
     9901 aaccctctaa gaaaactcaa ttaagagcga ttcatagagg gcttattatt atattatata
     9961 actttatata ctttttattt ttatatgctt caatttaact tatttcaagc atattcaagt
    10021 acttctcatt aactttatat acatcctttt ctctagataa aatctctatt tcttcaagat
    10081 tattaaacac ttccatcaac tggtcaacta attcgatttc ttttattcca gcctttaagt
    10141 atctaccttt attctttttg atatagttct ctaatgtcat agaataatta tctttactac
    10201 agatatattt taatatatgt tcttctgttg ctgatagagt tatatcattt tcaaataatt
    10261 cctcttttaa tatactctct aaagacttcc tatcatgcat atcacgcatt tcattgatag
    10321 attgtattat agactttagc atgaactcta taaatacagt taaattatat cctttattct
    10381 ctacatctag tatagcctta taatacttag ttctgttttt agatattata tatgatattg
    10441 aaaactcttt aaaagtatca tatcctttat taattaaata
//
LOCUS       DAEPLF010000119        10251 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEPLF010000119 DAEPLF010000000
VERSION     DAEPLF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10251
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.119"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..298
                     /locus_tag="KR314_002453"
     CDS             <1..298
                     /locus_tag="KR314_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0328828.1"
                     /translation="DDKLSIDSISSEFFISKYYLMRKFKSHTGTSIHNYIVQKRLILA
                     KSLITNGYLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYINNKNPLDITLLDE"
     gene            complement(512..2584)
                     /locus_tag="KR314_002454"
     CDS             complement(512..2584)
                     /locus_tag="KR314_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0328829.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLSHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYASIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKLLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNCLSTISPVVFTNLEQNQKLSIKKGITTAKEENRVSIL
                     RYDIEKIG"
     gene            complement(2887..3729)
                     /locus_tag="KR314_002455"
     CDS             complement(2887..3729)
                     /locus_tag="KR314_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0328830.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEISTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(3976..4470)
                     /locus_tag="KR314_002456"
     CDS             complement(3976..4470)
                     /locus_tag="KR314_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBH0328831.1"
                     /translation="MSTLVTLLGLLVCTKISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKLSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPRMPLSVSLALAAVISSTDLVAVKSITQGLNFPKNMMSILEGESLLNDDDRII
                     NIME"
     gene            complement(4622..5593)
                     /locus_tag="KR314_002457"
     CDS             complement(4622..5593)
                     /locus_tag="KR314_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBH0328832.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPDEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTRALMSAVPVQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(5594..6571)
                     /locus_tag="KR314_002458"
     CDS             complement(5594..6571)
                     /locus_tag="KR314_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328833.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMVIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPVLGKNKDKRLYSIPGQV
                     PNPIGIPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            6790..8349
                     /locus_tag="KR314_002459"
     CDS             6790..8349
                     /locus_tag="KR314_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0328834.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKRKEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            8455..9414
                     /locus_tag="KR314_002460"
     CDS             8455..9414
                     /locus_tag="KR314_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0328835.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            9417..10229
                     /locus_tag="KR314_002461"
     CDS             9417..10229
                     /locus_tag="KR314_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0328836.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVISLTIISKKFSSNT"
BASE COUNT         3575 a         1596 c         1252 g         3828 t
ORIGIN      
        1 agatgataag ctttcaatag atagcatatc gtctgaattt tttataagta agtattatct
       61 tatgagaaaa tttaaatctc atactggaac gtctatacac aattatatag ttcaaaaaag
      121 attaatttta gcaaagtcac taataaccaa tggttacctt atgagtgaag tttgtaataa
      181 atgtggattc aacgattatt ctagttttgt aagggcattt aaaaaaaact acggtgtatc
      241 tcctaaaaat tatataaaca ataaaaatcc tttagatatt actcttttag atgaatagat
      301 aatttatgta aattttattt taataaaaac ttgaagtatt agtgtattta tcttactaat
      361 acctcaagtt ttttaatgtt ttaaaatata tcaaaatata atgatatata tttctattac
      421 ttaaaatata tattaaataa attgcatgtt aactgaacct atttatatat ttattaaatc
      481 tcacataact tttagttata ctatcatata gctatcctat tttttcaata tcatatctta
      541 atatacttac tctgttctcc tcttttgctg tagttattcc ttttttaatg cttaatttct
      601 gattttgttc taaattcgta aatactacag gacttattgt tgatagacaa tttttttcaa
      661 tatattctaa gtccatttta actaattttt ctcctgcttt tacttcatct cctacattca
      721 caaatacttc aaatccattt ccttctaaat ttacagtttc tatccctaaa tgtataagta
      781 tttctactcc actttttttg gattttatta ttattgcatg cttagttgga aatataactc
      841 caatagttcc atcaactggt gaataaatca atccatcact agatttaatt gcaaatccat
      901 cccccatcag tttacttgaa aatacagaat ctggcacttc ttctatatct aatatctctc
      961 catttgttaa tgaatcaatc ttttctacta aagcattttc catattggca acttccatat
     1021 tttttttcac tttattttta tcaacatttt tagtaacagt actttcaacc tttgtttttc
     1081 cctctattat atcttttata tcagacttta ttgaatccga ttttggtcca aatatagctt
     1141 gaacattgtt gccaataacc ataacacctg ctgctccaag taatttcaat ttttctttat
     1201 ctactttatt tatatcattt acaaccaatc taagtcttgt tatacacgca tctaggctct
     1261 ttaagttgtc tttatttcct aaagcaatta aaatctctga tgctaattca ttcttagaca
     1321 tttcaatgac ttcagcgtta gtttctgact catcttctct tcctggtgtc tttaaattaa
     1381 attttcttat cataaatcta aatacactat agtataatac tgcaaaacca atacctacca
     1441 agatagccaa gtaccatgga gttctattag gaagaacacc aaataataag aaatctatta
     1501 atcctcctga aaatgtgaat ccaattttaa ctcctaataa ttgcataatt gcgtatgata
     1561 aacctgaaaa taaacaatgt attgcaaata atactggtgc aacaaataaa aacgcaaact
     1621 ctattggctc ggttattcct gttaaaaaag aggttaatgc tgctgatgcc attatacctg
     1681 ctacaaattt tttcttttct ggctttgctt catgcaccat agcaagagct gctgctggaa
     1741 gaccaaacat cataaatgga aatgcacctg tcataaaagt acctgctgtg aaatttactc
     1801 catctttaag ttgagcaaag aatatctttt ggtcacccat tacaagctca ccagctttat
     1861 ttacatattc tccaaattga taccaaaatg gattatggaa aacatgatgc aaaccaaatg
     1921 gtattaatag tctttccaaa gaaccttcca taaatgctgc tagtgtttga tttgcttcta
     1981 taacattttt tgagaaacta agtaatccct cttgtattgg aggccaaata aaagacaata
     2041 taattcctat tgctatagca actaatgaag taacgattgg tacaaatctt ttacctgcaa
     2101 aaaaacctag atactgaggt aattcaattt tataaaactt tttataaaga taagcagcca
     2161 ctatccctat aataatacca ccaaataccc ctgtttgaat tgttggtatt ccaacaatac
     2221 ttgcatacat agaattgtct accatatctt ctgtaacacc cgccataaca cccatagatg
     2281 tattgagaat taaaaaacct actatggctg ccaatgctgc aacaccttct ccttcagtta
     2341 aaccaacagc taccccaact gcaaatataa gtggtaagtt attaaatata atttgacctg
     2401 aactttccat tatacttatt ataagttgaa atgtaggatt cgccaaagct ggaaaatgag
     2461 acaacaatgc gtctcctgct aaagcattac tcacgccaag taatatacct gctgcaggaa
     2521 gtaatgcaac tggtagcatt agagattttc ctactttttg taaaacacca aatacttttt
     2581 tcatttactt ttctctcctt attattttat atttttttaa ataaaaaaga cttaagttta
     2641 taaaaagtta cattagtata cttaattcta aaattaaact tatgttaata taaataaacc
     2701 tatattaacc taatttcaga attaaaaata aactattata atttttttat aaacataagt
     2761 cttgcctgca ttatcagtaa caatcttaat gttttaaaat tattatttta tattaaattt
     2821 taaataatat attaaaatta aacataatat attaaaccta tacattctgt attaaactac
     2881 tctttattat aaattcagta cagatataac tcttttgata tgtaatgtta aataaagtag
     2941 ttcatcttga cttacatagt aatcataatt cttttctaca tgagttttta ttttatatgc
     3001 acaatcataa tccttttcat actttacttt aattatatct ataatttcat tatttgtatc
     3061 attgattttt tcttttttta ctaatcgttt tgcaaagaat tttaaatgag tcaaaagcct
     3121 atcataattt atatcatctt ctttaaattc aactctataa taatatctta taatatttag
     3181 tatgtctttt acaattttag taattaacag tgattccttt gaacttccct tatagtttga
     3241 atttactata tgcattgcta taaaaccagc ttcatctact ggaaatttca catcaaattc
     3301 tttatttata taatctacag cccatttacc aatctcatat tcttttttat gtattctttt
     3361 tatctcatgt agtaaatcat tctttatagt tatattttca tgatatcttt taattgcaaa
     3421 tgcaatatgg tcagtaagtg ctacataaat atattcataa agcttagtat tcaaattctc
     3481 ctctgcataa gcaattattt cagtactaat ctcaaaaaat ttctcatcaa cctggcttgc
     3541 tagtttgcta attttatcta aaaagctttc atcttgtatt acaaaagttc tttcaatttt
     3601 acttttatct atttcttgac ctttcttttt ttgaaaccca atcccacatc ctgttagtat
     3661 taattctttt tttatatttt catctaaaac taatacaaca ttattattat aaatttgttg
     3721 aataatcata atcaatatct ctcccaaaat acaatttccc ttattcataa aaaaatacct
     3781 aaattaagat acaattcacc tgtatacctt aacctaggta ttgcctgctt accagtaaca
     3841 atccagcttg ttaattctta tatattgtat tcaatttatg gttttattct attataaata
     3901 aatatatatg tcaagtaaat tattgaaaac tatttcctat tttttagtat tatttttaac
     3961 atcaaaaatc aaattttatt ccattatatt tatgattcta tcatcatcat ttaataaaga
     4021 ttcaccttca agtatactca tcatattttt aggaaaattt aatccttgtg tgattgattt
     4081 tactgctact aaatcagttg aagatattac tgctgccaaa gcaagtgaaa cagataatgg
     4141 catcctagga ataagaaaat gtataaagat tccacctgca aacacggtta tcaaaactaa
     4201 accaaatgca agaagcaaaa ttggttttct aagctctagt aactcttttc ttgatacatt
     4261 ttgtccttca ctgaataata atggagctat gatacatatt acaaatattt ctggattaaa
     4321 tgaaaatgat aatttaaacg gcaaaataga caatataatc cctagtacaa tttgatatat
     4381 tgcaagagga atattactcc actttttgct aaatacagtt gaaatctttg tacaaaccaa
     4441 taatcccaac aatgttacta aagttgacat aactacctct cttttcaata ataatattat
     4501 aaattaaact tatatatcaa ctaacttaat attaacaaca tgaaatccca tcttaatcaa
     4561 aaaaattttc agtttattct attattctta ttatatacat caaatttaat ttttgaaata
     4621 tctatactag atggcatgca acaaaatgat ttcttgatat ttcttttaaa actggaatct
     4681 cttttttgca tatttcctta caataagggc atcttgtatg aaacttacat ccacttggtg
     4741 gatttgttgg agatggtatg tctcctttta atataattct atttttctta attgtagggt
     4801 cttgtactgg tactgcgctc ataagtgctc ttgtatatgg atgttgagaa tgtaagtaaa
     4861 gttctccttt agttgcaatt tctattatag aacctagata cataacacaa actctatgtg
     4921 caatatgatt tactacactc aaatcatgag atataaataa atatgagagt ttatatttat
     4981 cctgtaaatc cataagtaaa tttataattt gagattgaat agaaacatct agtgcagaaa
     5041 caggttcatc acaaactata aattctggat ttaatgctaa tgctcttgct atacctatcc
     5101 tctgtctttg acctccagaa aactcatctg gttttctatt taaatggtac ttagaaagac
     5161 cacaactttc tacaacattt aatatattat cttttatttc attctttggc actaaattat
     5221 gttctataac tgcctctgat attatttgtt ccacatttaa tcttggattt aatgaactat
     5281 atgggtcttg aaatatcatt tgcattttgg gtcttaactt tctaagttct tccttctcta
     5341 acttatatat atctacacca ttaaattcaa ctttaccttc agttttttca attaatctaa
     5401 ggatggttct tcctgcagtt gattttccac atcccgattc tccaactata gccaaagttt
     5461 ctcctttttt taaatcaaaa gaaattccat ctactgcctt tacacattta atttccttat
     5521 caaataatcc agatttaact tcaaagtact ttttaagacc ttctactttt aataaactct
     5581 cttctttttt caattatatc tcctcctttt tatataacca acatgcaata ctatgaccac
     5641 tattcaattc tattaaagga ggtatctgag ttctacattt atcacatact ttttcacatc
     5701 tatcactaaa ataacatgaa tctggtattc caattggatt tggtacttgt cctggaatcg
     5761 aatacaatct tttatctttg ttttttccaa gaacaggctt tgattttaga agtccaatag
     5821 tataaggatg ttttggattt ttaaataatt caacaacctc tgccacttct ataattttac
     5881 cagcatacat cactaatact ttgtcacaca tttctgcaac tactcccaaa tcatgtgtga
     5941 tcatcattat agatgtgttt aatttttctt ttatattttt cattatatct aaaatctgag
     6001 cttgaatagt aacatctaaa gctgttgttg gttcatcagc tataagtaat tttggattac
     6061 atgataaagc catagctatc attattcgtt gtctcatacc acctgataat tcatgtggat
     6121 atgaattata tatttcttca gctcttggta tttctaccag ttttatcata tcgattacct
     6181 ttttcttagc ttcttccttt gataaatctt gatgtatcat aataccttca atgatttgac
     6241 ttccaacagt gaacgctggg tttaaagatg tcattggttc ttgaaaaatc attgatatct
     6301 cattccctct tattaccata agttcatctt cattcatctt taaaatgtct ttgcctcgaa
     6361 agattatttc tccaccttct atttttgatg ttggaagtaa cttcatcaaa gacatggctg
     6421 ttacagactt accacatcct gattctccaa ctattccaat agtttcacct tcatttatat
     6481 taaaacttac ttcatttaca ctctttacaa tccctttatc tgtatgaaaa taaactttta
     6541 aatctttaac ctcaattagt gctttactca ttattttctc ctctcagcaa aattacttaa
     6601 aataacatac accttttgat tgtattttat aaaaatttaa attacaacat tttcttacat
     6661 tttattattt ttttctatta attataataa tataatagtt aatttctata tatattgatt
     6721 taataatgat tactattata caatgaaaaa tatgtgttaa atattatttt atggaggttt
     6781 tataagaata tgaaatttaa aaaattggca tcattaatct tagtttcatc attaatgctt
     6841 acatttacag cctgtgcttc caatgataaa gacaagtcta gtggaagcaa atctggtgga
     6901 actgttgtaa tacctatggc aagtgaccca gatataatca atggagcttt tgcaaacgtt
     6961 aaagaagact ctcttgcatc aaatatagtt tatgctcctc tttatactta tgaaaaaggg
     7021 aatttagtaa attatttagc agaaaaagta gattttaaag atagtaagga attaactata
     7081 aaattaaaat ccaatttaaa atggcatgat ggaaaaccaa taactgctga agatgtctta
     7141 tttacattta atacagtttt agacgaaaaa caaaattcac catcaagaca atacttattg
     7201 gtgggtgaaa aaccagttaa agtagaaaaa attgatgatt taacagttaa gattacttta
     7261 ccaactgcaa gtgaatcttt cttatatggt atatctaaaa tttctccaat accaaaacat
     7321 gtatttgaag gagaatcaaa tatagctaag tcagaaaaaa ataataatcc agttggttct
     7381 ggtgcattca agtttaagga atggaaaaaa ggtgaaagca tagtatttga aaagaatgca
     7441 gattattttg gtggagaacc taaggcagat tctatagcgc taaaaataat tcctaatgaa
     7501 gcatctcaag aagcagcttt aaataatggc gaaatttcac ttatgaagac atctgctgaa
     7561 ggatatgaaa aggcaaaatc aaactcaaac ttacaaactt atacttatag tgaagaaaga
     7621 ttaaactaca ttgtatttaa ccaaaatata tcaaacatgg ctaacaaaga agtaagacaa
     7681 gccttatcat atgccttaaa tagaaatgaa atgatagaat cagcatatgg aaaagaaggt
     7741 tctgtacctg ctaaatctat attagttcca gaagcagact tctatacaga agaaggtgtt
     7801 gaaggatatg accaagatac aaataaggct aaagacttat tggataaaag tggtgttaaa
     7861 attgataaat taaaaatagg ttataacaca ggtagatttg ggcataaaaa ctatgcctta
     7921 gtagctcaac aagagttgaa aaaaattggt atagaagctg aaatagttcc ttatgaaagt
     7981 aaggcattct ttaatatact atttagtaat agcactgaat gtgatatgta tgtaaatggt
     8041 tatgcttggg gattagaacc aaacccatat agaggtatgt ttgaaactgg tcaatattgc
     8101 aaccaaacta aatattctaa tgctgaaata gatgctttat gggaaaaagg atttactgag
     8161 ttaaacaagg aaaaacgtaa agaaatatac aaacaaatac aacaggatat atctaaggat
     8221 gcacctattt acactataga ttatgaacaa aatttaatgg cagctcaaaa aaatctaaaa
     8281 ggaataaaag atgctaaacc atctcctgca atattatttg aagattggtc taaactgtat
     8341 gtagaataat tgatgtaaaa ttttatatta ttaattagaa aaggagatgt ctaaaaataa
     8401 tattgtttta ttattttaaa acgagacatc tcttttaatt aggagaattt acttatgtta
     8461 aaattaatat tgaatagagt aaaagtttta atacctatgt taatcttaat atctatatta
     8521 tcatttggac ttttagaatt agctcctggt gacccagcag atgcatatat aaatccttta
     8581 atgtcttcac aggatattga aaatataagg gttaatatgg ggcttgataa accagtatat
     8641 ataagatatt taaactggtt gaaaaacact ttaagtggaa acttaggtat ttcttatata
     8701 aaccatatgc ccgttacaga acaaattatg gaaaaaatgg gaaatacatt tattcttatg
     8761 ggtacttctt tgatattttc aatattggta gctatacctc ttggaatatt tttagctgta
     8821 aataaaaatt caataactag taaagtatca tctgttttta attatatagg tgtttctatc
     8881 ccttcttttt ggataggtat gatactcatt tcaatttttt cagttaaact taacattttc
     8941 ccatcaggag gtatgcatac tattggaaat gatagtattg gagatttagt aaaacattta
     9001 gttttgcctg taataacttt aggtctatat aatactgcta tttttacaaa ttatgtagaa
     9061 gctagtgtaa atgaacaatt aaaaaaacaa tatgttatta cagcaagagc aaaaggatta
     9121 tcagaaaaag tcatactatt taaacatgtc ttgaaaaatt cactgacatc attggttaca
     9181 atcctaggca tgagtataca aaaattagtc actggtgcat ttgttacaga agtagttttc
     9241 tcttggccag gtatgggaag attaatgatt gattcaattt tttcaagaga ttatactgta
     9301 attatggcaa taactatgtt atcagcacta tttttaattt taggtaattt agttgctgat
     9361 atactatact tattaattga ccctaaaata aaatctagca aaggaggatt ctagttgtgt
     9421 ttacaagtat cttaaaaaaa ttaaaaaaag ataaatcctt tgcttttgca tgtattactc
     9481 ttagcataat aatcatatta attatagctt cttttatatt aaatatagac tctaatgcaa
     9541 ttgatactac ttctaaactt caagaaccta gcttaaagca tatctttggt acagacgaac
     9601 ttggtagaga ttacttctct agagctcttt atggtggaag gatttcactt agtgttggta
     9661 ttttatcaat gcttatctct gtagtatttg gtactgctgt aggtgttatt agtggatata
     9721 ttggaggtaa gctagaccaa ttcttgatga gattagtaga tatgcttatg agtataccaa
     9781 gtttcttagt tttgattgta ttaaatactt atctaacacc taaagtttcc actattatac
     9841 taattattgg attcttaagt tggatggaag tttcaagaat tgtacgtggc gaaacattaa
     9901 aaataaagga aaatgaatac tgtttagctg ctaaagctac tggtatcaag actagaaata
     9961 tactattaag acatatagtt ttaaacttaa aagagactat tgtagtcgct ggttcgttaa
    10021 atattgctaa tgctatctta actgaatccg ctcttagttt tttaggtctt ggaatccaat
    10081 tacctcttgc atcatggggt aatatgcttc aagatgctca aagacatatt tttgacaagt
    10141 tatatttagc tgtatttcca ggattactta tatttttaac agtcataagt ttgactataa
    10201 taagcaaaaa attttctagt aatacttaac aaattttata tagaaaataa a
//
LOCUS       DAEPLF010000120        10225 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEPLF010000120 DAEPLF010000000
VERSION     DAEPLF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10225
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.120"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(184..1434)
                     /locus_tag="KR314_002462"
     CDS             complement(184..1434)
                     /locus_tag="KR314_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBH0328837.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLYEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDSDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1466..2407)
                     /locus_tag="KR314_002463"
     CDS             complement(1466..2407)
                     /locus_tag="KR314_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBH0328838.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2647..3723)
                     /locus_tag="KR314_002464"
     CDS             complement(2647..3723)
                     /locus_tag="KR314_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBH0328839.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4096..4611)
                     /locus_tag="KR314_002465"
     CDS             complement(4096..4611)
                     /locus_tag="KR314_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBH0328840.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(4974..6044)
                     /gene="leuB"
                     /locus_tag="KR314_002466"
     CDS             complement(4974..6044)
                     /gene="leuB"
                     /locus_tag="KR314_002466"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBH0328841.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDEIGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            complement(6051..6542)
                     /gene="leuD"
                     /locus_tag="KR314_002467"
     CDS             complement(6051..6542)
                     /gene="leuD"
                     /locus_tag="KR314_002467"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBH0328842.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKEKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6557..7834)
                     /gene="leuC"
                     /locus_tag="KR314_002468"
     CDS             complement(6557..7834)
                     /gene="leuC"
                     /locus_tag="KR314_002468"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBH0328843.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTITKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(7791..9533)
                     /gene="leuA"
                     /locus_tag="KR314_002469"
     CDS             complement(7791..9533)
                     /gene="leuA"
                     /locus_tag="KR314_002469"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBH0328844.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIKGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9657..9903)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10051..>10225)
                     /locus_tag="KR314_002470"
     CDS             complement(10051..>10225)
                     /locus_tag="KR314_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328845.1"
                     /translation="QSQKFNYKIIEKIEVVKNILNIYTDESNYKIRLRALSLEDEKKL
                     LNIIDEKMKKFKA"
BASE COUNT         3070 a         1984 c         1067 g         4104 t
ORIGIN      
        1 atgaattttc atcctcaatt atagcataga atatgactat ctatatccta aaattatcca
       61 tttctaaaaa aaggtaacta ttattaagaa tatcaactta tagaatttat attccacata
      121 cgattttcaa tcaaaaaaac aagggctaaa aagtgaattg tacacttttt agccctttat
      181 taattattca tttatttcat gtaagaatag cccacatctt actttaggtt caaaccatgt
      241 tgacttagct ggcattatct tatttgcatc tgctactctt attaaatctt caatactagt
      301 aggatacatt gcaaatgcta ctttcatatc ttcattaact cttttttcta attcttcaag
      361 acctctaatt ccaccgataa aatcaattct tttatctact ctaggattgt caattcctaa
      421 aatagcatca ataacatagt cttgtaatat agaaatgtct aatgagtcaa cagggtcttc
      481 tatttctaat aaatttttat ttgctttcat tttataccac ttatcaccta ggtacattcc
      541 gaaagttcct tttttgtcag gtttatattt ttcttttcct tctatttctt ctagttcaaa
      601 attttctttt attttattta taaattcttc ctcgcttaaa ccatttaagt ctttgataac
      661 cctattataa tccaaaacca ttaaattctc atctggtgca atcattgcta tgaagtaatt
      721 aaattcttct ttgccagtat aatttggatt tttagctctc atttctttgg ctacattttc
      781 tgctgaagct gtcctatgat gaccatctgc aatgtataga taatctaatt tttcaaattt
      841 atctactagg ttatctatta tatcatcact atcaatcttc catactgtat gctctatctt
      901 gtcatctgtt atgaaatcat ataaaggttc attattatcc ataaaatcat tgattatatc
      961 atctatcacc ctttgatgtt tgtaagttac aagtatagtt cccgtattag catcacagta
     1021 ttttatgtgt ttagttctat caatttcctt ttctggtcga gtgtactcat gtttttttat
     1081 tgttccattc aagctttcat caactgatat acatgctact ataccaactt gagctctacc
     1141 atccatgatt tgtttatata tatatataca ctctttgtca tcttttacta gaatttcttg
     1201 ttttcgaaac tcatataaat tttctcgcgc ttttaaataa actttttcat catataaatc
     1261 tatattttca tctaaatcaa tttctgattt atctatatgc aaaaatgaat atggattccc
     1321 ttctgcaatt cttctagctt ctttactatt cataacatcg taaggaagtg ctgctacttt
     1381 atctactata tatttatttg gtctaatcgc cttaaatggt ttaatcgttg ccattactaa
     1441 tttcctccta attatacaaa atattttata atgctacccc tattagattg ttgaagtcaa
     1501 gaaagaaatc ttgaatcaca tttactattt cgtctcctat tctttcttga gcttctactg
     1561 tagatgcccc aatatgaggt gtaggagaaa ctcttggatg atttactaag tctaaattta
     1621 atgtaggttc ttgttcataa acatctatag cagctgctga taatttacct tcatctaatg
     1681 cttttagtaa atctttttca tcaactaaac cgcctcttgc acagtttatt aaataagccc
     1741 ctttcttcat cttatttatt tctttttcag ttattactgc ccccttattc ttatcaaatg
     1801 gtatgtgtaa agataagaaa tctgcatttt ctaaaacttc ttccatatca cagaatttat
     1861 aattgttaaa accttgcgcc tcacccatta tgtcagtata tataacattc atacctaata
     1921 attctgctct ctttgcaact tcttttgcaa ttcttccaaa acctataagt ccaagtgtct
     1981 ttccattaat ttctgtacct ttatacttct tcttctccca ttttccatct ctcatagtta
     2041 catttgcagt atttatgaat cttgcaagaa caaataattg acctattgta agctctgcaa
     2101 cagatatact gcttgcattt ggagtattca taacttttat gccattagct tgagcatatt
     2161 taacatctat attatccaga cctactccac ctcttactat caacttcaac tttttaccct
     2221 ctaatgcttt atctattaca tctttagtaa ctttagtagc tgaacgtact acaagtacat
     2281 caacatcttt aagtttatct cctaaaacat ctttttcata aaactgctct atcacattaa
     2341 aatctaactc tctcaatttt ctcgctgctt ctttctctat tccatcagta actaatatat
     2401 tatacataac aaactcctcc aatttttatt cacttaattt ttccaatttt tattttttaa
     2461 agtattttca tcaataaaaa actttactta atttaacttt ctgtttatat aattttcaaa
     2521 atattttttt attaaagtca tatcaaattt tcctattgtt tgttaaaaat ttaatatgta
     2581 atttttataa aatattattt attatatatt ctaaatttat cttaaaacat acatattttt
     2641 acaatattat ttattaaaac ctaatatatc atcaatatta tctaataatt cttgtacatc
     2701 ttcgacagta tagtcaccca tatgtgaaat tctaaaagtt ttttctttta aatctccata
     2761 gccatttgct atttggaatc ctcgttcttg taattttgaa tttaaatttg atacactaat
     2821 accttgagta ttttctataa cagtaagtgt attggataag tgattttcat tggtaaaaat
     2881 ttggaagtgt tttttagccc agtttctaac taagtttgcc atgtcttcat gtctgttgaa
     2941 tctgttatct aggccttcat ctaatatatt atctaattgg aaatctaaag cgaacatgtg
     3001 agaaagagat ggtgttgaag ggtattggta gtttttcttg attaagtatt catacattgc
     3061 taacaaatca agatatactc ctctaaaagg tacttgctta gctctatcga ttgctttttg
     3121 agaaaatgtg caaatagaca ttcctggtgg taatcccagt gctttttgta ctgatgttat
     3181 acaaatatct attccaatct tatcaacctc gacttttatt ccaccggctg aacttacagt
     3241 atctacacag tagattacat cttcatactt tttaacaact tctccaattt cttctattgg
     3301 atttcttatt ccagttgaag tttcattgtg agttacagtt attaaatcgt attctccggt
     3361 agaaagcacc ttatcaacca tttctggcgt tgttgcctct cctaattcaa ctttaaatat
     3421 atccgctggg acattattag ccactgccat tttgtaccat ctatcaccaa aggatccaca
     3481 ggaaaatact gctgcctttt tggatgtaca ggaacgaatg gagccttcca ttaaaccagt
     3541 tccggatgac gtggataaaa gtatcgtgtt gtttgtatag aatagttttt gcatcttttc
     3601 acttatactc ttttggagca tagatgcatc ttttcctcta tgtcctatca ttggtgtagc
     3661 catcttttgc aagacttctt ctttaacatc aataggacca ggaataaata atttcttact
     3721 cattataatt cccccctttt tagtttgtaa tatggatagc gtttttactg tccttatttt
     3781 taataactat aatatcaagc tgaattaact atgtcaacat aaaatttatt ttttatatat
     3841 aatttatttt atatatttta tgaaaacatt cattttctgc cttttcataa gtctgaattt
     3901 tctgaattat tctctcaaaa aaaatatact ttttatctat ataaattaat ttttttagta
     3961 tgtaaatttg acgctattac gataaatctt tttagtataa ctaatttatt tttatcttgt
     4021 atttttattg tatttttgaa ttttattttt tctaaatata aaatttatgt tttaaaatat
     4081 aaaattttta tttaatcaaa gttcaatata ttccactttt tctttcgatt cttcaaaaaa
     4141 ccattcttca ttagaaaaat caccttctgc aatttgtcca ataggcattt tcatatactt
     4201 tccagttgct gtcactgcta tatctccatt tggtagaatt atttctcctg taccttcaaa
     4261 taattttctg ttttctttag ttattcttcc aacaactttt atagtagcat ctgttggtac
     4321 tggtttctta tatttcaact ctaaagatac tgttactccc catatttggt catcatttat
     4381 acttacagct cttccaatag tctcatctaa tattgcagca gacatacctc catgaactct
     4441 cccaggataa ctttgatgcc aatcctttgt attacaaatt gatacaagct ctccattttc
     4501 aagttcataa aaatctgctt taagtcctag ctcattttgt gttccacaaa ctaaacaaga
     4561 tttactacta ttttgcttct tcaaaacttt atactttgcc aaaaaatcca tctcctaacc
     4621 gacattagta gtcgcttaat attaaataat tctaaaatat attatataat attaataaaa
     4681 tccctgcaaa cttctaataa taaaaatagg ctacttcaat atgataagtt taatttaaag
     4741 tattattttc caaaaaataa ctttatcaaa atagaaactc aaagtagata taagcctctt
     4801 tttcgctatt aaaatagtaa aaatatattt gacttaatat aaaaaagagt gtctcattaa
     4861 ctaataaagt ttattttgag acacccctca taaaacaaat tatactacaa tagattttta
     4921 ctaattctac aagttatata tttaaattat attattcaaa ttaaactata attctaaatt
     4981 tcatttacta ttaattcgcc cattgctctt gttcctacaa catttcccac accattatag
     5041 atatcaatag ttctataacc tttattgagt acactttgta cagcatcttc aatacactta
     5101 gcttcttctt ctaagttaaa actatgtctt aacatcatgc ttacagatag aatagttgca
     5161 attggattta caatatcttt tccagctata tcaggcgcac ttccatgtat tggttcatac
     5221 ataccaaaac tatcttctct aagtgatgct gatggtaaca tacctataga acccgtaatc
     5281 atagatgctt catcagatat tatatctcca aatatattat tagtcactat tacatcaaat
     5341 tggctagggt cttttacaac ttgcatagca gcattatcaa catacatatg tgacagttct
     5401 acatcctcaa actctttagc taagtcttcc attatacttc tccaaagtct tgaactctca
     5461 agcacatttg ccttatctac acttataagt tttttatttc tatttctggc tatttcaaaa
     5521 gctctcttac caattcttct tatttcattt tcgttatata tttctacatc ataagcattt
     5581 tcgataccat caatgatttt tcttccacgt tctccaaaat aaatgccccc tgttaattct
     5641 cttacaacaa caaaatcaat gcctttctca actatatccg ttctaagtgg tgaagcttct
     5701 tttatacttt catacattgt agctggtctt aaatttgcaa ataaacccaa tgactctctt
     5761 aattttaaaa gaccagactc tggtctttta tctcccttac atttatccca ctttggtcct
     5821 ccaacagcac caagcaatac agcttcgttt tttcgacaag tttcaacagt tttattaggt
     5881 agaggttctc caatttcatc aatcgcgcat cctccagcta atacatattc acaatcgaat
     5941 ctatgattaa atttacaaca aattttattt agaaccttta taccttcatc tataatttcc
     6001 ggtccaacac catcaccttt tatgactgct atattacaat tcatactcca ctacccccta
     6061 tttgctttta ttgagttaat taagccttca tttaatataa tattttgcat aaactcagga
     6121 aatggttctg ctttatattc tttttctttt gttatatttt ttatcacacc tgtagaaaaa
     6181 tctacttcta tattatcccc ttcatctatg ttattggcag cttcttcaca ttcaagtata
     6241 ggaagcccta tatttataga gtttctaaaa aatattctag caaaagtaga agcaatcaca
     6301 caggatactc cactttcttt tattacaatt ggtgcatgtt ctcttgatga accacaacca
     6361 aagtttttag ttgcaactat aatatctcct ttttttacct tagaaataaa tttatcatca
     6421 atatcctcca tacaatgcgt tgctagttct ttataatcag ctatatttaa atatctagct
     6481 ggtattatta catctgtatc tatattgtca ccgaatttaa aaacacttcc attggcaatc
     6541 ataaatttta cctcccttaa attatttctg atggttttga tatttttcca gttatagctg
     6601 atgctgctgc tacagctggg cttgcaagat acacctctga atcaacatgc cccattcttc
     6661 ctacgaaatt cctatttgtt gttgaaatgg atttttctcc tgctgcaagt atacccatat
     6721 gtccccctag acatggacca caagttggtg tagaaactgc tgctcctgcc tctataaaag
     6781 tggtaatata ccctttttct atagcttcaa gatatacttt ttgagttcca ggaaatatca
     6841 taactctaac tccatctgct acttttttgc ctttcattat ctcagctact acttcaaggt
     6901 ctgatattct tccatttgta caagaaccta ttacaacttg gtctatcttt atatccttat
     6961 ctatctcatc tactgttcta gtattttcag gaagatgagg aaaggctact gtttctttta
     7021 gttcggctaa atctatttcg tatacttcat catagactgc atcttcatct gcttcatata
     7081 tatttacatc ttttgttata gttgaacact tatgtgaatt tatatattcc atagtcttat
     7141 cgtctactgg aaagattcca ttttttgctc ctgcttctat tgccatatta caaatagtaa
     7201 atctatcatc catagataag tattctactc catctccaca aaattccatc gacttgtaaa
     7261 gtgctccatc aacacctatc tcccctatta tatgaagtat tatatcttta ccacttatcc
     7321 atttatttgg tttattcttc aaaacaaatt ttattgctcc aggaacctta aaccaacatt
     7381 ttcctgtagc catacctgct cccatatcag ttgaacctac accagtagaa aatgctccta
     7441 atgctccata tgtgcaagta tgagagtcag cacctataat tacatctcca gcagttacta
     7501 tacctttttc aggaagaaga acatgctcta ttcccatatc tcctacatca aaaaaattta
     7561 caactccttt tgattttgcg aaacttcttg taaatttaca ttgttctgca ctctttatat
     7621 ctttattagg tgtaaagtgg tctaaaacca tcactacttt agtcttatca taaacatctt
     7681 caattcctaa tttttcaaat tctctaatgg ctactggtga tgttatatca ttacctaaaa
     7741 ctacatctaa atctgcttcg attaattgcc ctttcttaac ttcatttaat ttagcatgtt
     7801 tggctaatat tttctgcgtc atagtcatcc ccatttttta tttcctcctc aatataactt
     7861 ttatttattg cattcataag tgcattaata gctgctgtat ttatgttttc cgatatactt
     7921 acaccaaaat agacttcatt attgtgctct attcctacat aagctacccc tcttgaatgt
     7981 gacccttcct ctaatgcatg ttcccagtaa tatctaaatt tataatcttt catgccacat
     8041 tgctttagtg cattattaaa tgaatctaca ggtccatttc ctataccaac aatcctttgt
     8101 tcatttccca tataacttat tcttgcagtc atctcaatca tgttagtatc attagaatca
     8161 tcattttcat agtttaattc atccattgat tttattttat acttcttaac tttataaggg
     8221 ctctcaactt caatatattc tttcttaaat aaattaaata ttgcttcact actaagttct
     8281 tccccaattc tatcagattc cattttgact acatttccaa aatcagaatg catattctta
     8341 ggtatcataa atccataatc tgtttccatt atataaacag ctcctccttt tccagattgg
     8401 ctatttatcc ttataatttc tttgtattct cttccaatat catgagggtc tattggtaaa
     8461 tatggaactt cccagtaatc attatctctg tttttcattg ctatcatgcc ttttctaata
     8521 gcatcttgat gtgaacctga aaaagcacaa tgtacaagat tacctgcata tggatgtcta
     8581 tcatgtacca taagttttgt acagtcttca tatataccaa ttatcttatc tatattgcta
     8641 aaatctagct ctgggtctat tccttgtgta taaagattaa gtcccacatt tactatatcc
     8701 atatttccag ttctttcacc atttccaaat agtgttcctt caagtctatc ggcaccagcc
     8761 aataatccaa gttcacttgc agccgtacaa gttcctctat cattgtgagt atgcaaactt
     8821 agtataatag aatccctaca acttatattt ttacaaaacc attctatttg gtctgcatat
     8881 acgtttggtg ttgccatttc cactgttgat ggaagattta ttattacttt tttttgagga
     8941 gttggtttcc aaacatctat cactgcttca catatttcaa gtgcataatc catctctgtt
     9001 cctgtaaaac tctctggtga atattcaaaa gtaaattctg tttctggata tttttttact
     9061 tcttcattaa ataattttgc tccttcaact gctatatcta ttatttcttg tttactcata
     9121 ttaaaaacca catctctttg aagaacagat gtagagttat ataaatgtat aattgctttt
     9181 ttacatccct ttattgattc aaatgttttt tctataagat gagccctaga ttgtgtaagt
     9241 acttgtatag ttacatcatc tggaatcata ttctcatcta ttaattttct taaaaatgta
     9301 tactctgtat ttgatgctga tggaaatcct acctctattt ctttaaatcc tgtatcaata
     9361 agcattttga acattctgac tttttcattt acactcatag gcgttggcag tgactgattt
     9421 ccgtctctta aatcgacact acaccaaatt ggagccttaa atatttcatt atctggccat
     9481 ttacgattta ctatttgcat tttatcatat tttttatact ttccacactt catgttattt
     9541 cccccttttt ctgttttttg tattcagtat accttatatc gacgcaatcc tgcacacttc
     9601 ataaggataa atgtcaattt tatcattaga ataaaaaaaa cctcatctct aataaaatta
     9661 gagacgaggt tttgctcgcg gtaccactct atttagcttt ctaaaaagct cccttagtaa
     9721 tagccaatac aataattata atgactatat ttcctataac gtggaattac tccgattatt
     9781 ctaagattta aatcttagaa aacagctcct agacaagttc agaactatca actactaaat
     9841 cacaccatcc tttagctctc tgaaagtttc attgttctta ctacttctat tcatcacttt
     9901 tttaatcatt taaattttct aaatattttg atttaatagt tattatatac acattaaaat
     9961 gtattgtcaa caaaaaaaat aattatctca attttttatt tttgataagc aattctataa
    10021 aacatgctaa ctattgaaaa cagtatgata ttatgcctta aactttttca tcttttcatc
    10081 tatgatattc aataattttt tttcatcttc caaactcaat gcccttaatc taattttata
    10141 attagattcg tcagtgtata tatttaatat attttttact acttctattt tttctattat
    10201 cttataatta aacttttgac tttgt
//
LOCUS       DAEPLF010000121        10224 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEPLF010000121 DAEPLF010000000
VERSION     DAEPLF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10224
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.121"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(145..666)
                     /locus_tag="KR314_002471"
     CDS             complement(145..666)
                     /locus_tag="KR314_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH0328846.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENIIIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
     gene            794..2158
                     /locus_tag="KR314_002472"
     CDS             794..2158
                     /locus_tag="KR314_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0328847.1"
                     /translation="MKESNKKIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDKLHIAIKTSILWTTIFCVVLGL
                     ILVLFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            2347..3024
                     /locus_tag="KR314_002473"
     CDS             2347..3024
                     /locus_tag="KR314_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBH0328848.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSDYKKGNPVH
                     CGDSDCIGILIIKSGTIRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQINSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(3504..5189)
                     /locus_tag="KR314_002474"
     CDS             complement(3504..5189)
                     /locus_tag="KR314_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328849.1"
                     /translation="MKYLIEIAKKERLLVFIYLALGIGIELLNSFSANYYQSLIDRFN
                     NSTINFHIIFIYGTVLVTLCLLRYIDEYPGRRLEHSFFLDLKLKALEKMSKIDYLEYQ
                     MLGTGKLIQKIENGANAGRNIFFNFLLAVVRKIIPSILFSMVFIYRIDKKVMFTILIG
                     YFIVFIITNLLIKALYQIKEHILINEEKMNHFLVRGFVEMVVFRVNKRFEYEICQSKF
                     AKKEIIDSRVKMTLIHEAFFTIFSLIVTFIKMLIIVYSWKTKTISIGSIIALITLLDN
                     AYTPIAIFNVLYIQFKLDKAAYKRYTDILELNNDGQLLAGKEVNLMYGNIEFNEISFL
                     YNNKREIFNKLSLSIKSGEKVAIVGESGSGKSTLIKLLIGLLKPSEGTIFIDDYNLLE
                     MNLNSYYNYVTYVSQDSPIFDGTLRENIVFGKTIKSNDIIEVLEKVGLKNLYLKLEAG
                     LDTKLGEKGTTLSGGEKQRLALARLWFSDAKIIILDEATSALDNITEEKVMLNVMEFL
                     KDKTTIMIAHRFNSIKNVENIIVLKEGSIIGSGSFKKLLDGNLYFRELYYTMS"
     gene            complement(6112..6693)
                     /locus_tag="KR314_002475"
     CDS             complement(6112..6693)
                     /locus_tag="KR314_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0328850.1"
                     /translation="MNNKKHWYDYLWIFSSIYLVLGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(6686..6916)
                     /locus_tag="KR314_002476"
     CDS             complement(6686..6916)
                     /locus_tag="KR314_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0328851.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            complement(8241..8516)
                     /locus_tag="KR314_002477"
     CDS             complement(8241..8516)
                     /locus_tag="KR314_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0328852.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            complement(8592..9893)
                     /locus_tag="KR314_002478"
     CDS             complement(8592..9893)
                     /locus_tag="KR314_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0328853.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
BASE COUNT         3442 a         1704 c         1070 g         4008 t
ORIGIN      
        1 aaaagtttaa cattctccct caattaagtg agctttattt taaatatact tttcagttta
       61 tatcatttgt ttcatttttt ctaatatctc attattaaat atgctggttt ttgtttaatg
      121 attattaagt tagcttctct aattctaatt ccccatatta tcttttaaag aatttatcca
      181 ggtttccaat gcttgtgcta gcaccaactg ttgctccaac actgcttttg ctgtctctgg
      241 tacttctgtc tttataatta tattttcctc aatagttttc tttaactctt gaacattctc
      301 ttcaatatta ttgagacata atttctgttt ttctggtgta aggctattta aatttactat
      361 aacagcatta aaatctaaaa gaatatttat tggtttgcta gatgtatcaa acattaaatc
      421 taaaaaatgt gtctcaccta attcagttaa tgaataaact gctttttctg gcatttttcc
      481 attcttcaca gttttacttt caatatatcc cttttgttct aattgaatta cttttttata
      541 aattgatggt gtacttattt taacccaacg tgatatattc ctgtattcca ctgccttttg
      601 aatatcataa gcactcttgg gttgttgctt tactattccc aatacaatta aatctattgt
      661 agccataaaa atcatctcct ttagttttct tactattgac aaatactata aattatagta
      721 ctattttcca tacttactat aatttatagt attataattt agacttaatg tcaatagaag
      781 aggagtgtta agtatgaaag aaagtaacaa aaaaattgaa ttattagggt ctgccccaat
      841 ctcaaaagca ttgttatcta tgggattacc taccatgata ggtatgatga taactgcatt
      901 atacaatttg gttgatgcat actttgtggg tggacttgga acaagtcaaa tgggagctat
      961 atccgttgca tttccattag gtcagcttgt tgtgggatta ggattattgt ttggaaatgg
     1021 agctgcttca tatattgcca gacttttagg ccatggtgat aatgaaaaag caggaaaagt
     1081 ggtaagtaca tctatttaca gtagtattat agtgggtaca ttagttatta ttgtgattat
     1141 gatttttctc aatccaattt taaaattgtt aggagctaca aaaagtatta tgccttatgc
     1201 aatatcttat gctagcattt atgtcgtgtc atctattttt aatatattca atgtcactat
     1261 gactaatatt gtcacaagtg aaggagctgc taaaacaact atgtgtgcta tgttattagg
     1321 tgctttgctt aatatgattt tagacccgat attcatttat acttttggtc tgggggtaag
     1381 gggtgcttct attgcaacag ctatctcaca gattgcttca acacttgttt atgtaggata
     1441 tattttaagc aagaaaagta tatttagttt tagaattaac gaatgctgtt tttcaaaaga
     1501 aattttttca gaaatcctaa aaattggcat acctacatta atatttcaat tattgactag
     1561 tcttgccatt acattaacta atatggaggc tacaaagtat ggtgattctg tgatagctgg
     1621 aatgggtgct gtaacacgaa taacatcttt aggtagtctt gttgtatttg gttttataaa
     1681 aggtttccaa ccaattgctg gttataattt tggttcgaag aactacgata aattacatat
     1741 agcaataaaa acttccatat tatggactac aatattttgt gtagtgcttg gtcttatatt
     1801 ggtgcttttc ccaaaatcta taatttcaca atttacaagt ggtgacattc tattaataga
     1861 aaatggtagt aaagctctta gggcaaatgg gttttcattt atgctatttg gattctatac
     1921 agtgtattct acattattcc tagcactagg aaaagcaaag gaaggtggat ttttaggtat
     1981 gtgtagacaa ggcgtctgtt ttatccctat aattttgatt attcctgtat tctatgggtt
     2041 aaatggtatc ttatatgcac aaccaatagc tgatttgatg gctgctataa ttgctggttt
     2101 tatgtctata aggttacata aggaacttaa tcttttaaaa ataaacggta tgcattagtt
     2161 atacaaacct actatcatca aaacatatta ctttctcatt aatagcaaga atatatatta
     2221 tcatattttt tctacataaa tcatactatt actatctgac taaataatcc tcttaacaaa
     2281 ctacttgtca atttatctat tcacttgtat catgggatta taacaattta agaaaggaga
     2341 tattgtatga tttcacaaaa tgaaattgat atacttacac atagcttgcc tttttgggat
     2401 aaacttactg acttacaaaa agaattatta atttcttcag caaatacctc ggattacaaa
     2461 aaagggaatc cagtacattg tggtgatagt gactgtattg gtattttgat tataaagtct
     2521 ggaacaatac gtacatacat actttcagat gaaggtcgag aggttacact gttccgactt
     2581 gatgatggag atgtatgtat tttatcagct tcctgtattc taaaaacaat aacttttgat
     2641 gtctatgtag atgctgaaac ggactgcgat attatacaga ttaattcctc tgtttttgca
     2701 aaattatcta cagaaaatat tcatgcagag ttattttcat ataaattagc taccgaacgt
     2761 ttttctgatg ttatgtgggc tatgcaacaa attcttttta tgagttttga taagcgtttg
     2821 gcatcttttt taattgatga gatagctaaa aatggcagta gcactataaa tatgactcat
     2881 gagcaaattg ctaagtatat gggtagtgcc agagaagttg tatcgagaat gttgaaatat
     2941 tttgctagag aaggtattgt gtctttatct cgtggtggta taaaagtatt ggataaggat
     3001 aaacttcgtt ctttgacact gtaaaagtgt gtctcaaaat gaacttttta gttcatgagg
     3061 cactcttttt tgtgtgaaat caaatatatt ttcataattt caataataaa aaagatactt
     3121 atatctattt ttatatagta tcttatttga tatttacatt tattttttat gtatattttt
     3181 atattttaat aatttagttt aactattttt ctatgctatt tatatagaat atttctaata
     3241 actcataaat tctaatcttt taatatctat ccctcaatta atggatatga atttatttaa
     3301 tttaaatact tgatattttt catatttata taaaaatatt acattttaaa tacaagttaa
     3361 taaaaatgga agaatatatg ttaatgtaaa tattaagaat aatactaaaa aatattactg
     3421 gaatctcaaa tttttaataa tattccagta atatttaaat aatattaaat taagaaaaat
     3481 attttcttat acttacataa taattatgac attgtatagt ataattctct aaaatataaa
     3541 ttaccatcta atagtttttt aaagctacca ctacctatta tactcccctc tttaagaaca
     3601 attatatttt caacattctt aattgagtta aatctgtgag caatcataat agttgtttta
     3661 tctttcaaaa attccataac atttagcata actttttcct ctgtaatatt gtctaaagct
     3721 gatgtagctt catctaatat aattatctta gcatctgaaa accaaagcct tgctaatgct
     3781 agcctctgct tttctccacc tgataatgta gttccttttt cacctaattt agtatctaaa
     3841 ccagcttcta attttaagta taaatttttt aaacctactt tttctaagac ttcaataata
     3901 tcattacttt ttatagtttt tccaaataca atattctcac gtaaagttcc atcaaatata
     3961 ggtgaatcct gtgacacata tgtaacataa ttatagtaac tatttagatt catctcaagt
     4021 agattataat catctataaa tatagttcct tcgctgggct ttaataatcc aattaatagt
     4081 ttaattaaag ttgatttacc agaaccactc tctccaacaa tagctacttt ttctccactt
     4141 ttaatactta aactaagttt attaaatatc tctcgcttat tattatacaa gaagctaatc
     4201 tcattaaatt caatatttcc atacattaaa ttaacttctt tacctgctaa cagttgacca
     4261 tcattattaa gttccaaaat atctgtatag cgtttatatg ctgctttatc tagtttaaat
     4321 tgaatataca atacattaaa aatagctata ggagtatatg cattatctag taaagttatc
     4381 aatgcaatta tagaacctat acttattgtt ttagttttcc aactataaac tattattaac
     4441 atttttatga acgtcactat taatgaaaaa atcgtgaaga aagcttcatg aatcagtgtc
     4501 attttaaccc tagaatcaat aatttctttt ttcgcaaatt ttgactgaca aatttcatac
     4561 tcaaatcttt tgtttacccg aaatacaacc atttctacaa atccacgaac taaaaaatga
     4621 ttcatcttct cttcattaat taatatatgc tctttgattt gatatagagc ttttattagt
     4681 aaattagtaa taataaaaac tataaaatat ccaattaata tggtaaacat aactttttta
     4741 tcaattctat aaataaaaac catactaaat aaaattgatg gaattatttt tctaacaacg
     4801 gctaataaaa aattaaaaaa tatatttctg ccagcattag ctccattttc aattttttga
     4861 attaattttc cagtgcctaa catctgatat tctaagtaat ctattttact cattttttct
     4921 aaagccttta atttcaaatc taaaaaaaaa ctatgttcta gtcttctacc tggatattca
     4981 tcaatatatc ttaaaagaca taaagtaact aatacagttc catagataaa aattatgtga
     5041 aaatttatag tactgttatt aaatctatct attaggctct gataataatt agcactaaaa
     5101 ctatttaaaa gttctattcc tatacctagt gctaaatata taaaaactaa taacctctct
     5161 ttttttgcta tttctattaa atatttcata actgtacacc tatccttatt tattttactt
     5221 tattaactta aaataatttt atagataaaa tgtttcttta tttgtttaaa tttagtgtga
     5281 ttattaatca tttcaagtta acaaaaccta aagtttttag cagtacagtt ctatttgtta
     5341 gcaaattatt attttactca gtacattatg ctaacatata atctaactgt actgagtaaa
     5401 tatctcgttt catcatataa aatctccttt ttatagttta atatatatag ttaaatttac
     5461 tatataatct gaatatttat ttttctatca cctttaccag aaataaaatc aattatttat
     5521 tgtataagca aaacttcata atattttcaa catataatta ttatacagaa ctcctaagtt
     5581 aatttaaagt tagaatttat ttttataaga aatgtcattg acctaccttt aataaaacag
     5641 ccaccatttt tttatgtatt aacatataat atatagtaat gaaaacatat taattaaaaa
     5701 atattgtata aattagttta tctgtaacaa atccagttta tgtttcaaca agcacgtaag
     5761 acttcttgaa atttaatctg cttcctttaa atatctatta cttgttttaa gcataagttt
     5821 acaatcttct ataacattta tcatttcttc agatttctct gacgtactta aaatacaagt
     5881 tgttacattg tttttctatt taattagtaa tgtatcatgt aaaaaagaag cataaatgag
     5941 tttactttat acaaacttca tttatgctcc actcatatac ctatcatagc taatagaaac
     6001 tattactcat cttgaatcta tttcatattt agatatttat atctttctta ttttcttaaa
     6061 tcaactctag ctttaaacat taaacccctt aaatcttcac aaaatctctt cctatacaga
     6121 cttactcttt gccttacata tcatctgagt catagtaccc atagggcaat aaacacacca
     6181 tgaacgaggt ttaaacaata tcatagtaat caaaccaaga acagttgatg taagcataac
     6241 actataaaaa ccaaatgcaa actgtaccac ccaaggtgaa actaaagttc catgatacgc
     6301 ccattgccaa ggcattttta tagtccacaa caaagtcact acttctttaa aatttctact
     6361 tccttcaaac actaaataag ttgaaaaaag catattggca aacattacca taaaaaatgt
     6421 caaaaaccca tatctaaacc acttacttct aataaattta ggaatatctc tatttcttga
     6481 cagcttcaat ttatttccaa gcaaattaaa cagttgccct cttccacaat acttattaca
     6541 gtaagcttta tttcccttaa caatagaaat agctagtgga acaaagaaac aaattagccc
     6601 aatccaagcg aaaataatat ttacaaatcc caataccaaa tatattgaag aaaaaatcca
     6661 taaatagtca taccaatgtt ttttattatt catgacactt accttcttcc tcttttaata
     6721 ttgttataat ctcaattaca gatgctggac atatcttttc acaaatccca catccaacac
     6781 ataaatctct attaattttt gcactaatgc cacatggaac tgagatagca cttcttggac
     6841 atgcttttat acagctacca caagcaacgc actcatttgc taaaatattt gcatacttct
     6901 tttttttatt agacataata acctccctat attattaatc tataagaatt atatcaagat
     6961 acttactttt tatctgtgac ttaatcacat aaaacctcaa tattaaaaat cacactctaa
     7021 atacaaagga gtttcaaata aaaaacgata tgaagtcatt aagtttagct tcatatcgcc
     7081 ttttcttttg tgtatctata taaatattta ttatctaata tttaatatat ctataatatt
     7141 ttcttataca gctctataat tttacaccta atcatattct attagtaaca ggttaatacc
     7201 catctaaaac tataacttta ttcacaatct tattctatta gccacaagtt aaactcatat
     7261 tgaagtttat ctttagttac actcttactc tattaatcac aagttaagcc catattgaag
     7321 tttatctcta tttacactct tactctatta atcacaagtt aagctcatat tgaagtttat
     7381 ctctatttac actcttactc tattaaccac aagttaaact catattgaag tttatctcta
     7441 tttacactct tactctatta atcacaagtt aagctcatat tgaagtttat ctctatttac
     7501 attcttactc tattaaccac aagttaaacc catattgaag tttatctcta tttacactct
     7561 tactctatta accacaagtt aagcccatat tgaagtttat ctctatttac attcttactc
     7621 tatcaatcat aggttaagct catattgaag tttatcttta gttgcacctt actctattaa
     7681 tcacaagtta aacccatatt gaagtttatc tttagttaca ccttactcta ttaaccacaa
     7741 gttaaaccca cattgaagtt tatctctatt tacactctta ctctattaat cacaggttaa
     7801 acctattata atcttaactt tattcacaat tttattctat tagccacagg ttaaatctat
     7861 tataatctta actttattca catattacat cattaatcgc atatcaagat tttataataa
     7921 gctttaacta aacacacgat tttaccttat tcgtatagtt taatctatta aattattata
     7981 taatcttaag caaacataca actctacact attaattata gcccctcctt ttaaatactc
     8041 ataatcttat acacaacctt accttattaa tcacacattt aactcatata tatcatcaat
     8101 tttattcaca aaatcatgct gttaatcaca cttatactcc tctataatct taaacttata
     8161 cacaaattag ccacaatttt atttcaacta taataacttc tatttcagaa gtatatcaca
     8221 cttgtattct ccaaaaatat ctattttttt ctaacaatat gcctgacatt aataacactc
     8281 atttcaaaca tcctcgctaa ttccatataa tttttcccat taaacaattt aatcataagc
     8341 tcatttctag cctcttttaa tgccccttcc agtgttttaa tataaaaata tgttccccca
     8401 ctttgttgac acaattcaag gtattccaat aaactatact tatgtaactc ctttggaata
     8461 cttacttcta tcttgtgttc ttcaagtaat tctataattt tcgttaattc caacatattt
     8521 tcacctcata ttttataatc tatttgctat ttcctgactt ttaataagct tgttattact
     8581 actttattga cctaataatt tagttcttga aaatgtaact tatccccaaa gtaacaaaaa
     8641 tgcttatgcc tagtctgtcc atctctacac ttcgcaacta ttaaatcaac catcttaatt
     8701 cccctgtatt ctgatgctct aactgcctcc tcactttctc caatatctct cactgcttct
     8761 tcataatcac tcccctttaa ttgatgtata taaaccacat tgttgctatc gtggtagatg
     8821 gctttgctat ccctcatagg tctatctcca taaggtcttg aatctttcat ttcatcattt
     8881 aactgactta attggattac aggtatatta aaatctaaag tcatattttt aagttctctc
     8941 gaaaaagtag ctacctccct ttctctctta tccatgcttt tctgagatgt aaggagttgc
     9001 acataatcca ctattaataa atctggcttt acctgcctta tcctctttct aattgcacta
     9061 attgtatcta tcttatcatt aatataaatt aagttatttt cgcttaaatc acctatagca
     9121 tttacaataa gtttccaatc attttcatct atttctttag atttcatatt ccttgtactt
     9181 acaccagtta ctctgcatat atttctcata aatacttgct cactggtcat ttctctactt
     9241 ataaataaag tttttttacc ttgcttaaat gaatttaaca ttatttgaag tgccaaagca
     9301 gttttcccaa cccccgattt agctgctatt gtggttagct ctcctttaaa aagacctcct
     9361 atagtagtat caagtaattt tataccaaat ttaaatccaa tatctttttt gttttctaaa
     9421 aaatcaagca ccttccctgc tatactattt acatcatcat tttcataagt atccttgtcc
     9481 aaaatctcct ttatattact ttcaaatttg taaatacaag tatcgatatt ttctccaagg
     9541 tttaggctgt gtaaaaggtt ttggcagttt tgatttatgc ttcttctagc taaattttct
     9601 tttaaaattt taatatgacc atcaagacca aaattctgac cccaagtaat tagattagtc
     9661 acatcactcg tttttattgc tagagatttt ctatctatct ttgatttcac agtggccaca
     9721 tcaatcacta tgttttcttt tttaagttct ttcataattt taaatatctc aagacatata
     9781 tcattcataa acatgttttc atttaattca tctagcttat agcttaattt attgtctaaa
     9841 agaattgtgc ctaaaataga cttctctatt tcaacatctt ttgatttacc catataaatt
     9901 aatctctctt tccttataag tttttatatt ttgattttta aatacattat caattataaa
     9961 tacattattt gttatgactc actatttgtt atgactatat tattcgtcat gatatactac
    10021 aaattattaa tatactattt attctgatac atataattta ctacataata gcaatatctt
    10081 atttgatata acttactacc aattattaat acattgttca atataattca ttactcgata
    10141 gtaatatctt atttgatata acttactgcc aattattgat atatcgttca atataattca
    10201 ctacctgata gtaatatctt actt
//
LOCUS       DAEPLF010000122        10183 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEPLF010000122 DAEPLF010000000
VERSION     DAEPLF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10183
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.122"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(62..931)
                     /locus_tag="KR314_002479"
     CDS             complement(62..931)
                     /locus_tag="KR314_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0328854.1"
                     /translation="MDYKTIILNRKSVREYKNIDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNKSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(1437..1658)
                     /locus_tag="KR314_002480"
     CDS             complement(1437..1658)
                     /locus_tag="KR314_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328855.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(2159..3139)
                     /locus_tag="KR314_002481"
     CDS             complement(2159..3139)
                     /locus_tag="KR314_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328856.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRVYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(3303..4175)
                     /locus_tag="KR314_002482"
     CDS             complement(3303..4175)
                     /locus_tag="KR314_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0328857.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(4339..4890)
                     /locus_tag="KR314_002483"
     CDS             complement(4339..4890)
                     /locus_tag="KR314_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0328858.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSYVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(4910..5812)
                     /locus_tag="KR314_002484"
     CDS             complement(4910..5812)
                     /locus_tag="KR314_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBH0328859.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EKMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(5827..8199)
                     /locus_tag="KR314_002485"
     CDS             complement(5827..8199)
                     /locus_tag="KR314_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBH0328860.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAEGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(8418..9107)
                     /locus_tag="KR314_002486"
     CDS             complement(8418..9107)
                     /locus_tag="KR314_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328861.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMAGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEEFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(9348..>10183)
                     /locus_tag="KR314_002487"
     CDS             complement(9348..>10183)
                     /locus_tag="KR314_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0328862.1"
                     /translation="MNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGASSIFLILL
                     FKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIYCIDNLRNN
                     SKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCSINFSKVNF
                     VKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITKSFFVLKCENSKLNQLTF
                     NKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPLEQDTDSWG
                     D"
BASE COUNT         3294 a         1784 c          954 g         4151 t
ORIGIN      
        1 atattattga actttttata aaaattaatt taagtgtagt taaattaagt attaaactat
       61 cttatatatt acaatatcct actatcttat agtcttctgg cactttataa tctttgtctg
      121 gagtacctaa attccatttt aaatcaaaca tagttgcact tattattgtt gcaaaataaa
      181 gcataactat accaatgtct attttttctt catataaatt agtatgacca tcttttctaa
      241 cagctaaaac tacagtgcct ccatcaacta taaatctcca tggttgtcta tttaatgtag
      301 atggagctaa tcttgcatag tgaaatgcat caagaagtgc tctttcttct aagatttcta
      361 tacttgcaac atttccccat tcgttcatat aaacaacatc ttctacacct agtctagtag
      421 accccttatc tgttacaact tgcatatcag attttgaata attttgccca gtttttgtag
      481 catttaatac tttagcatta cttacatttt ctccatatcc aatagctata attcctgtta
      541 cctctttatc tgaattaatt ccaagttttt cttttacctt ttttccatct tcaaaagtaa
      601 cccaacaaga accaattcca agttctgtta ctttaaagat tatactttct cctatgtacc
      661 ctgaattttc aatgtaatta tcttttatgt cagataatat tataatataa cttggagaat
      721 ctatcatatt tcctttgtac cctgctactt tatctaaatt attaaaaact tcacttttat
      781 tcataattct tacatcaaca tctatttctg gaactagttt cttacaagat tctgagtatt
      841 tcttaatttc actaagatat tcttctttaa tatctatgtt cttatattct ctaacagact
      901 ttctatttaa tatgattgtt ttataatcca taattttatc ctccccaaac tagtattgtc
      961 ttaattatat acccattaat atattatttt actcatttat ttaataaata aagattacaa
     1021 ttttgtaatt attatttatt aataatattc cacttttaca ttaataatga ccagttgtgc
     1081 aaaatccatt gccaaccacc aaataaaaac atattataaa agtaatataa aataacaaac
     1141 aaatcttaac aaaaaattaa acttttttta cacgaaagaa ggcttcccgt tgccttcttt
     1201 atttttataa aaaaataagc tgtttctaaa atgatttcac atttttgaaa cagccaagtt
     1261 attctataat gacaactata atttgaggat gtgagggaga gttgtcatta taaatttagg
     1321 gtaattatat tatgttttaa gctagttctt cctatattta tataatacat tttatatatg
     1381 aattttatat gagattggta taaatgatat atgaaattac atatgtttat attatattaa
     1441 attttattat tacattttcc acaacaatac tcatatgtct ccacattatt tggaatattt
     1501 acaaatacaa attctttaca gttctcacaa tactttaata tagatatagt tccacaagtg
     1561 taacattcaa atattacaca atcctcattt tcctctacta tttcaaactt tttactatgt
     1621 tcaccacact ctgggcactt gtgattattt acattcataa atactcctcc ttattagtat
     1681 agttacattt caaaaactta attaatgtat tctatattac ttattatata ttctataata
     1741 aatcatattc taatataata aaacaaatta aataaaaaaa aaccataaat tttaaattta
     1801 tggattaatt cttgtaatga taactctatt tgaggatgtg agggagggtt atcattacaa
     1861 tagggtatta tattatggtt tttaagctag tctttctaca cctcatatga tacatatgct
     1921 atatgaattt tatatgattt ttacataaat attttatgaa atttaaattt tttagtcatt
     1981 ttgaaatttg taaaaagttc tctaaaaatc cacagcatca taaacagcca aaatttaagt
     2041 aaaacatcat aaaaaaattt gtccaataat tttaacaagt catttcacct cattataatt
     2101 attgacacaa acttaaataa acaatctata aatcttatat aaatcttaaa ctttaccctt
     2161 attgatacac aatttctagt ttgtcaattt tattttgcat ctctaagaaa atttcttcaa
     2221 cagaattgtt aaatatattg cctaaataga ctcttttatc tttttgaaat gaataagatt
     2281 ttacactacc atcagaagaa atatataaat gactccactc tgctccatat tttgaaaacc
     2341 tctcctttgt atctttaaac ctcatatctt ctatttttat tgctttttct ctgtctcctc
     2401 ttactacagt gtgacccctt tttattaaac tggttatttt tatatcttct tttaatttaa
     2461 tacgtgtatc atcatttttt gaatatactt tccatatcac tttatgacga taattatcta
     2521 attcttttat taagcttcta tcaatagtaa agctatcttt agttaccaca attaaatcaa
     2581 aatggtctaa catatattga aatatttctc tcatactttt attttttaaa atatcctcac
     2641 caataacctc tataaatcta cagcctttct catacatgca gttcaataaa cttgtgattt
     2701 cagaaaaata atcatttgta ttattatctc catctatttc tatagtaaag tacaaaggag
     2761 taatgtatga tttatttcct gttatctcaa atacacttga tagtgtctgt atatcatctt
     2821 caaacgttaa gtatacattc tcataaaaca taaaaaattc cataactttt tctttcatat
     2881 tatggatatt tctatatcta ttggataata tatttattat atcgtcagta gtatttttcc
     2941 catcacattc agccagtata acaatttgct tccaacttaa gctatccaca tttccgatac
     3001 tttttttata tccatcttct tcatcataaa taggaccaat tataatgttt tcttttggcc
     3061 acagatgaac cctagttcca tcttttaatt tcggatattt attttttaca gactcataaa
     3121 atctacttgt atcacccatg attctttact cccccccata gtttaaatat tctatgctaa
     3181 aaatagcact ttatttttat taattcaaca attattcaaa attggtcatt atatatgttt
     3241 aaatggattt aacatcaacc ttatttatat gttattgatg tttatatatg ttatataaaa
     3301 tcttatttct catacttcaa tgtaatctta actatctctg gtttacaatt aagcctaatt
     3361 aaaacttttg agcctcctaa gcctttattt aaaatcatag tgctattatc tttttcatat
     3421 tttccccagt catacttagg aaagaactct ctatttggag ataatacacc tccaacaaaa
     3481 ggtattctta ttatacctcc atgaacatgt cctgctaaaa ccaaatctgc tccccatttt
     3541 gcatattttt caaagaaaaa tggtgtatgt gctaaaagaa tgttatattc attttcatta
     3601 actttaccta gattgtcttc tacaaaattg tcacttagtt ctaatttttt gttattagca
     3661 aataaatatg gatagcattc caatggtata attaaaccat ataaattaag atggctatta
     3721 cctctatata ttcttacaga ttcattttcc atatttatta ttttctttga atatagttcc
     3781 ttaaaataat ccttatatag atgtttatac ttttttatta aagatttttg ttcatgattt
     3841 ccaattatat gatatacttc atatttttca accaaattat ctattaaatc taatgcaact
     3901 tgaaaatttt tattttctcc atctactaaa tctcctgtta taaatataac atctgggttt
     3961 aatgattcaa ttttatctat caatacctta ttatttttat caaacacctt attatgtaaa
     4021 tcactcactt gaactatttt aaagttatca aattcctttg gaattttttt gttgaaaata
     4081 gtatattctg tcgtttctat atgctgagtt tcatacttat acagaatata caataatatt
     4141 aatactaata ctgtcattga aacctttata atcatattct cttcctcttt attctatttt
     4201 gttaatatta ttataatgct tttatgtttt ttttaaaaca ataacatcat cacatacata
     4261 taaatctttt cgacattata aaaataagtt atccttttct aaaattttca tttattttag
     4321 aaaaggataa ctataaaatt atctataaac ctctatattg tgaaatatag tttttccttt
     4381 atttaaattt ttatatatat gctctaaagt tccatcaaac ctaatagaat gtccagactc
     4441 taaaatataa gttttcccat ccacattaag gtcaagttta ccttctatta cgagtacata
     4501 ctcttctaaa acataactat gtgtagatga aacatgagta cagttttcct caagctcaat
     4561 tataaaaagt tcaaaccttc tttgagggct aaaagggtat attggatata acttcatgtt
     4621 ccctttttgc tcaacaatag gctctacttc ttctttcttt ataacctcat aggtatttgt
     4681 attttcattt aatagtgtag taaaatttat tttaaatcca gatgaaattt tccataaagt
     4741 agatactgtc ggacttgatt cacctctctc aatctgtcca agcattgctt tactgacacc
     4801 tgtcatcttt gaagctttgt ctaaacttat gcccatttct tgtctcaata accttaaatt
     4861 ttctcccaca ttaggaaatt tattatccat gttaaaatcc tcctgatagc tatttgtatt
     4921 ctatgatttt tactcctgta agttctacta tctcttttaa ttttaacata tgattgttac
     4981 aaatttcatc aaattcatat atataatctt caagccctaa tgccttatat ttatcgattc
     5041 caaatttatg atatggaagt aactccatct tgcctcctgg aacattttgt ttgacaaata
     5101 atgcagtatt tcttatattt tcttcactat catttactcc ataaataatt ggcactctta
     5161 taaccataga tttattcaac tttgaaagcc tacatatatt atctaatata attttattac
     5221 ttactcctgt atattcttta tgaatattat catccataga ttttatatct acaaatatgt
     5281 ggtctatttt ctcaaataca tcatttacct tattccaatc aaaatatcca caagtttcaa
     5341 ttgcaatatt tatgccttta tcataaaaaa tatcaactaa ttctcttaaa aaatcaatct
     5401 gaagtgtagg ttcacctcca gaaaaagtta tccctccatt agattcaaag aaaaatataa
     5461 aatccttttc aacttcctct actatttctt caacactcat tttttcggtc attatacaaa
     5521 tggcatcttt aagacatacc ttgatacact ctccacataa atcacattta ttatttattt
     5581 gcgttctatc aaatagtgaa cttatatttt gaggacaaac atctatacat ctattacagg
     5641 atacacattt atctttcata acacctaatt ttactatgtt actccaggaa tctggattag
     5701 aacaccactt acattttaat ttacatcctt ctaaaaatat agtagtcctt attccatctc
     5761 catcatttac tgaaaaatgt tgtactttta caactctacc ttttttactc ataatctacc
     5821 tcataattaa aaattgctat gtgaatttct agctattata gcatcttgca tttctcttga
     5881 taaattaaca aattgagtac tatatccagc aactctcact aataaatctt tatattcttc
     5941 tggattttct tgagctttta atagagtatc atttgatatc gtattaaatt gtacatggaa
     6001 tcctcctaaa gcaaaatatg atttaatcat agatgttaga tttctaagtc ctctttcagt
     6061 ttccaccaaa tcttgattca gtcttaaatt tagaagtgtt ccaccagtat gaacatcatg
     6121 atttattttt gatgcagatt tcatggctgc tagtggactt gttgtatcag taccaacaaa
     6181 cggagaaact ccctctgtca aaggttttgt cgcttttctt ccacaaggag ttgcacctac
     6241 gatttttcca gttggtacat agtttgatat acccataaat gcactattaa atttagagcc
     6301 aaaaatatcc ttgtattttc tagtttcagt ataataaaaa tcagaaactt caattgctaa
     6361 tgaatctaca taatcattat catttccata cttaggaaca tccaacgcaa gttttcttaa
     6421 ttcttcataa ccttcccaat cattatttag tgcttttgtt aattcttcta gcgtagtcac
     6481 cttatcctca aatactaatt tttttatagc tgccaatgaa tttgaaacta cacctaaccc
     6541 tataccagtt aatacaggac ctatattata ttttgctcct cctttactta aatcaactcc
     6601 cttatccaaa catccatcta cacaagtaga taaaaatggt cttggtacca tttctttatg
     6661 aatttgttga gcaacagtag ttcctataac agaacttttt acaagatatg ctaattgctt
     6721 taaaaactca tccttaagtt cttcaaaaga agtaaattct gtgatatttt ttgtatctaa
     6781 ccccattttt tctccagtta atctactctt accttcattc atagcatatt ctaaagctgc
     6841 tgcaaaattg atatttacgg ctgaagtcca ctgtcctgtt tttctaaaat gaggaactac
     6901 acaaccacag ttactccaat ctctagcatc ttctgcatca tatccatctt gtaataacat
     6961 ttgtgcccct gcactatcgc tgtgtatagc agggaaacca gttccttgtt ttaccaattt
     7021 agcaactgcc attacgaaat tatctggtgc tccttgactc actctaacac ttaatcctgg
     7081 ctggtgagtt tttacacttt ctgtagcctt taaacacata tatgagatat cattcgttcc
     7141 atcagtacca tttctctttt taccaccaac agtaagattt tggaattgat tatatcctgc
     7201 aaagtaattt gctgtatttg ctgatatagt ccatacccat tctgataact taatccaaag
     7261 tgcttctatc aattcttgag cttctgattc agttatttta ccttctctag catcattttc
     7321 ataatatgga tacatatatt ggtcaaatct acctaaattt aatgccaatg gattttctga
     7381 tagaatacca cctaattgaa caaaccatac aaattgtatt gcttcataaa aattagttgg
     7441 agggtttgct ggtacattca tacatatctc tgaaattttt ataagttcta actttctctt
     7501 ttcatctgtt tcttgcttac tcatctcttt tgcaagattt gaatatcttt gtgccaatgt
     7561 cataataccc tctgcaacta tagaaacaga tttgtaaaaa tctatctttt caatattttc
     7621 tgaaactgat atatctaatt ctttaatttt tttatctgca tcttccttaa tccctttata
     7681 tccttttaca aacaatatat cttgataatc tggtgttact tcaccaactg cctgtctcca
     7741 cttagaatca ttatctatta ttcctgtatc tactaatatc ttagctgtat cttctgggat
     7801 tcttgctaag aaaacttctt ccaaagattt tcccttccaa tatggaaata tttcttttct
     7861 tataaattct atttgttctt ttgatattac atatgggtct tgagttcttt tatcaaaagt
     7921 atccatttct ttgtctaccc attgccaaga aaactctggt gtcaatatcc ctgttcttct
     7981 aaattttcct gctgtaccta ctattaattc attttcaaag atttttatag gaagttcttt
     8041 acatgtctcc aaaaaagctt tagctcttct tatacagata gcttctccct cagttttttt
     8101 atgtgattct gtaaaaattc tagccctttc acaacatatc ataggtttag aattaatata
     8161 ttcttgtcta aatgcttcta ttctattagt ttgactcatt taaaatcccc ctttggataa
     8221 tatatactac atttttgtat gttataacat actttaatta tattatactg tcctttagca
     8281 taaaatggaa gcaaaataac tttttttttc atctaccttg aacacctata tattaatact
     8341 ttttttgtta aatttataaa atctaaaaaa attttctatg atatattttc tatattgtta
     8401 tagttatttc taatatacta atgatttgaa atatcaaatt tgtatactag acttagtttt
     8461 aaaaccttag ttgattgttt aactttgtta acttcaacat gtatatcctt catataagga
     8521 agctccacaa cacctactcc cttattaaat tcttcttcaa ccatagaaaa aatttctttc
     8581 tcagttaagt tcgtattagt caatgattta aataaattgt atataaattt aatatattta
     8641 tcactctcag ataacttatc atcttctgga tactctttag cacagagaat atctattgtt
     8701 acttttcctt ctttatcgtc ttcagacata gaatagaata aacttcgaat atttttttca
     8761 ggtggttttt cagcaccatt tatagatact acaaagctac catctgattt tgagtcagta
     8821 atactataac cactgtctat tatagctttc gaaaatcttt gagataaatc tttttttact
     8881 tcttctctac ctttcgcatc attaatcttt tgtgagtaat tattaaatag ctcgatagat
     8941 tcatcttttt tatcttcaac ttgttctttc aatgaatctt tactaatttg ttctgagcta
     9001 cttaagttct tatcagatac aggagacata catcctgcca tcaatgctaa gctaagattt
     9061 aaagccacaa ttattgccac tctatttata ttccttttta ctttcataca caatcctccc
     9121 atcacactaa ccgctctaaa gtattattca ttgcattaga atatatttat attttcttta
     9181 tattaaagat ttattattca atacatatca ccttcctatc caattcttat aacttactat
     9241 tagtaaaatt ataagctcct taatacagaa ataaggatgt tatcttttgt tattaaaaga
     9301 taacatcctt atttttttgt atcaatacat atgtcccaac tggtaactta atcgccccaa
     9361 ctgtcagtat cttgctccaa tggtatatat atatttagta taaatttatc gatactattt
     9421 tcaaataata attctccatt atatttttgt aaagaagatt taatactctg aatacctatt
     9481 ccatgtacga acttatccat tttgtttgtt aacaatatat ttttattaaa agtaagctga
     9541 tttagtttac tattttcaca tttaagtaca aaaaatgact ttgttatagt tcctttaatt
     9601 ctaatatatt tattaatatt ttcatccact attttatcac atgcttctat tgcattatcc
     9661 aaaatattag aaaatatgct tgacacatct ataggtttta caaaatttac ttttgaaaaa
     9721 tttatagagc aagtaaattt aattcctttt tttaaacata tttcactctt ttcgttgatt
     9781 ataatgtcta atatcatatt tcctgtatta tagatatatt taaatgtttt tatttcatct
     9841 tttaaattgt taacatattc attaatttct ttgctattgt ttctaaggtt gtctatacaa
     9901 taaatgtggt tatttatatc gtgatatagt ttcttaactc gcatatgaga ttcttgaata
     9961 cttaaataat gcgcatattg catatctaac ttatttttta ttaattcagc ttgagatttt
    10021 aatctattct cttttattat ttctttaaac aataatataa gaaatatact actagctcct
    10081 accaactttg gattaacaaa attatcaaaa tagtatcctt ctgaatgtaa tgacccaaac
    10141 cgttctaatt tttttaatac ataatttaca agcatattca ttg
//
LOCUS       DAEPLF010000123        10152 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEPLF010000123 DAEPLF010000000
VERSION     DAEPLF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.123"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            259..1476
                     /locus_tag="KR314_002488"
     CDS             259..1476
                     /locus_tag="KR314_002488"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBH0328863.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1571..1951
                     /locus_tag="KR314_002489"
     CDS             1571..1951
                     /locus_tag="KR314_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBH0328864.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2235..2717
                     /locus_tag="KR314_002490"
     CDS             2235..2717
                     /locus_tag="KR314_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0328865.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVNLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2738..3553
                     /locus_tag="KR314_002491"
     CDS             2738..3553
                     /locus_tag="KR314_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0328866.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3604..5154
                     /locus_tag="KR314_002492"
     CDS             3604..5154
                     /locus_tag="KR314_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0328867.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5251..6603
                     /locus_tag="KR314_002493"
     CDS             5251..6603
                     /locus_tag="KR314_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH0328868.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAESLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKVFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6734..7492
                     /locus_tag="KR314_002494"
     CDS             6734..7492
                     /locus_tag="KR314_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0328869.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIYNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7539..8258
                     /locus_tag="KR314_002495"
     CDS             7539..8258
                     /locus_tag="KR314_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBH0328870.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8382..9782)
                     /locus_tag="KR314_002496"
     CDS             complement(8382..9782)
                     /locus_tag="KR314_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBH0328871.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9786..>10152)
                     /locus_tag="KR314_002497"
     CDS             complement(9786..>10152)
                     /locus_tag="KR314_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0328872.1"
                     /translation="AKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYFSLHNKSL
                     FFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAYLEKEDTL
                     RPKVLLLNSIKNQLKKETL"
BASE COUNT         3869 a         1190 c         1808 g         3285 t
ORIGIN      
        1 atataaattt taataaaaat aataaagtat atattattgc tttaataaga atgtaaaaaa
       61 tatttttaaa ttatcattta atgtaattta aatgaattga agatatatgt ttattgtatg
      121 caattgttgt atttttaggt tttttgtgat taattaaaaa attataaaaa tcgtttacat
      181 gttgattttt aaaaaaaaaa gatatagaat tatatagaaa gtaataaaca aaaattggat
      241 ttacgggagt ggaatgaaat gatgaatgtt accttaacag atgtgaaaga ggctagagag
      301 accattaaga acatagtaaa aagaactgac ttacttgaaa gtgttaaact tagcgaaaaa
      361 acaggagcaa atgtgttcta taaatgtgag aatcttcaaa aaacaggttc ttttaaatta
      421 agaggagctt gtaacaaaat agcgagttta acagatgaag aaaaggcaaa tggagttata
      481 gcttcaagtg cagggaatca tgctcaagga gttgcattgg gagcaaaaat gactggtata
      541 aaatctacaa tagtaatgcc agcaactgcg cctttagcaa aagttagtgc aactaaaggt
      601 tatggagcag aagtagtttt aaatggtgcg gtttatgatg atgcatatgc aaaagctgtt
      661 gaaatacaaa aagaaacagg agcaactttc ttacatccat tcaatgataa gtatgttata
      721 gcaggacaag gaactatatc tcttgaaatt ttcgaacaat tagacaataa agttgatact
      781 atactatgcc cagttggtgg aggaggaatc atctctggtg ttgctgttgc agcaaaagca
      841 ttaaatccta atgtaaaaat agtcggtgtt caaactgcaa atataccttc aatgaaagag
      901 tcaataaaaa atggaaaagt aacaacagca tttaatgata ctacaatagc tgatggtata
      961 gctgttaaaa ctccaggaga tttaactttt gaaataataa atgaattggt tgatgaaata
     1021 gtagtggttg aagagacaga aattgcagaa tcaatattat ttatgatgga aagtcaaaaa
     1081 atagtttcag aaggtgctgg tgctgtatgt acagctgcta tattaagtgg taagtatgta
     1141 ccagcaaaag atgaaaatgt agtttgtata atatctggtg gtaatataga cataaatacg
     1201 ttatatagaa taataggtgt tgctttagct aaagaaggaa gaagatattc atttagtaca
     1261 attatggaag ataagccagg aaactttgct gagttaacta gaataataag tgagaatggt
     1321 ggaaacattt taagtgctaa tcaaggtaaa ttatcagcag gggaagctct aggaaaacaa
     1381 agtgcggaat ttatattaga gacaatagat tatgaccata tagctagaat aaagaaagct
     1441 atagaagaaa aaggttttaa aattatagaa ttataataaa aagtgtatat aatatacaag
     1501 aaaaatagta taatagtgta tgaagataca ttttatgtgt attagattaa cgaattagga
     1561 ggattttaaa atgaaacatg aagtaataca tactaatgat gcaccagcag ctttaggacc
     1621 atattctcaa gctataaaag ctggaaactt attatttgta tctggacaag ttcctcttgt
     1681 accagagact atggaagtag ttgaaggaga tgtacaagct caaactgctc aatctttaaa
     1741 aaatcttaaa gctatattag ctgaaagtgg agctgatttt tcaaatgtag ttaagactac
     1801 agtatttata aaagatatga atgaatttgg ggctataaac gaagtttatg ctgaatattt
     1861 tggagaaaat aaaccagcaa gagcttgtgt agaagttgct agattaccaa aagacgttaa
     1921 agttgaaata gaagtaatag ctgtattata aaaatacaat atttttaatt tgaaaaggct
     1981 gaaaaactat attctagtga ttcagtcttt ttataaaaaa ataaatcaca ataaagaaca
     2041 tgataaatat tgtcgaatta aaaaataaaa ttaaaataaa tattgcaata tttttgagaa
     2101 ggttttataa tgagtatata atttgaaacg tgtaactatt aaattaggca tgagttcttg
     2161 gatattttta tcctgaggat tcatgccttt tttgtattga aattcaaaaa tctatttaat
     2221 aaggaggaag tattgtgtta aattttttca agaaaaataa aagctataag ttacatgcag
     2281 tagtgagtgg taacagcatt aatattgaga aagtaaacga ttctgtgttt tcaaaaaagt
     2341 taatgggaga tggagttgca attattccta atagtaatgt tgttgtagcc ccttgtaatg
     2401 gtaaagtaac tgtgcttaca gaatcaaagc atgcctttgg tatggtctca gatgagggtg
     2461 ttgaaatatt agtccacata ggaattgata cagttaattt acagggtgag gggtttaaaa
     2521 atgaagtgtc tcaaggagat actgttaaaa aaggtagtcc aataataagt tttgaaagag
     2581 aaaaaataaa ttctcaggga attgattgta caacaataat aattgtatta aatcatagcg
     2641 aattttcaga aataaattgc atggtagaaa atgaagttgt tgcagggcaa gatactgtta
     2701 ttgaaattat gaaataatag gaagggatta attcaatatg agagtagtaa aaatctttaa
     2761 taataatgca ttatctacag taactaatga taggaaagaa gctattttac taggtcttgg
     2821 tataggtttt aataaacgtc caggagataa agttaatgaa gataaaatag aaaagattta
     2881 ttatgttcaa gaccacatgc aaacaaaatt tttagaatta ttaaaaaatg ttagtccaga
     2941 agtgatggat gcttcacaac aaattataag tcttatacca ggaaatgagg gaaaatttaa
     3001 taataaaggg atattatctt tagtggaaca tattagtttt gcaatagata gaatgaaaaa
     3061 taatgttttt ttacctaatt taatgctttt ggatataaag atgatgtatt caaaagagtt
     3121 tgaattagga gtaaaagctt tagaaattat atatagagta tgtcatatcc atttgcctga
     3181 agatgaagca ggttacatag ctttacattt tgtcaattta caatcaaatg ataatttggc
     3241 atatgatact ttaaaatttg taaaaggtag tattgatttg attaaagaat gttatggatt
     3301 agaattagac gaatcgagtt taagtacatt acgttttaga actcatctta aatttcttgc
     3361 tcaaagaatt tttaagaatg aaatttgtca ggatgataaa atgattgaaa tgtatgatta
     3421 tttaattaac aatcatccta aaaataaaga atatttagaa aagttaaatg tatatattga
     3481 aaaggagttt aggtataaat tagataaacc tgaaaaaatt tatctactac tacacctaac
     3541 aaaaatctta taagaatgag atgaattagt aatagaaata tataatttta gggggggata
     3601 agattgaaag aaaaactact tggaggactg gaaacatttg caaaggcaat ggttcagcca
     3661 ttaatgtatt tatcgagtgc aggaatatta atggtggtag gtgtactact tacaaatgat
     3721 atgttattag gagtacttcc attttttaaa tgggagccaa ttcaaatatt tggaagccta
     3781 atttataact gtataatggc tattattaat aatcttgctg ttatatttgc tataggtatt
     3841 cctgctgcac tagcaaaaaa agagaagcac aaagcagcgt taataggatt tatgagttat
     3901 ataatatacc ttacagcttc caacactatg ttaacatcac taggtcaact agcaaaagaa
     3961 agcaagatgc ttggactgat agggactgga caagctacta ttttaggtat tcaagttgta
     4021 gatacaagtg tatttggtgg aattatttta gggtgtatag ctggatatgt atttaatcgt
     4081 acaagcaaca aaacatttaa aggagcttta cagatatact cgggagcaaa ttttagtttt
     4141 atatgcatgt tctttgtatc cattttaatt ggagttgtga gtaactttat atggccatta
     4201 gctcaacaag gaatagcttc tcttgcaaat gtcatcaaag atacaggtac atttggttta
     4261 ttcttatatg gttttttaga gagaatatta gtaccaactg gacttcatca tttaatctat
     4321 acaccatttc agttttctga tttaggagga gtgcttaaag ttggtgcaga tacagtttca
     4381 ggagcctatg ctatagttat gacagaaatg aatttaccag ttgagcgttt ctcagatagt
     4441 atatactaca tggcaactgg atttacaaag atgtttggtt atataggtat aggagctgcc
     4501 tttatttata ctgcatataa gcaaaataag aagaaagtta aggcaacgat tattccacta
     4561 ataattacgg cagtattagc ctctgtaaca gaaccattag actttatgtt tgtattctgt
     4621 gcaccacttt tattctttgt acatgcagtt atagctggtc tttttatagc tttattaaaa
     4681 atatttgatg ttactgcatt atgtggaggg aacttattag tatcaattat aatgaatact
     4741 acagtaggtg tagaaaaaac acactggcca gtaatgatga tattaggact tgcacaaatt
     4801 attgtatatt ttgtagtatt tagtttttta attaagaaat ttaattataa aactccaggt
     4861 cgtgaagatg agagtacagc tacaggcgaa gaacaatcaa aacaacttaa tttagatgca
     4921 ggtatagaaa atataattga tgggttaggc ggaaaagaaa atattaatac agtagaaaat
     4981 tgcatcacta gattacgtgt taatgtaaaa gatgaaagta agattaatga agatataatt
     5041 aatttaacgc caaatagcgg gattgtaagg aaaggaaaag atatacaaat aatttttgga
     5101 cttcatgtgc atgaggtaag acgtgcagta gaagattttt tagaagctta ttaaagatat
     5161 atatttatat agcaagggta aataacagct ttaagtgctg tttactattt attaaagata
     5221 catataattt atagaggagg aataattatt atgattatta ctattgttgg tggaggaagc
     5281 acatttactc caggtattgt taagtcaatt gcgttaagag aaaaagagtt agagcttgag
     5341 gaaattcgct tatttgacat tgataaagaa cgccaagata aagtagctgt agttgtcaac
     5401 tggatactaa aagaagatgt taaaacagat gttaaactaa ctgtaactaa ttgtgaaaaa
     5461 gaagcgtata cagatgctac atttatattt gcacaaatgc gtgttggtaa atatgaaatg
     5521 cgtgagcagg atgaaaaaat tccattgaaa tatggttgtg taggtcaaga aacttgtggt
     5581 tgtggaggga tggcatacgg actgcgtaca atacaaccaa tgataaaggt tattgatgat
     5641 gtagagaaat atgctaaaaa agattattgg atattaaatt actctaatcc tgctgcaatc
     5701 gtttctgaag cttgtcgtaa gttaagacca aatgcaagga ttataaatat ctgtgatatg
     5761 ccaatagcta ttatagatat gatagcggag tcattaaata tcaatgacgt tcataatata
     5821 cgctatgatt attttggtct aaatcatttt ggctggttta cttcaataga ttataaacat
     5881 agagacttaa tggaagagat taaggaatac ataaaagaaa ataaaatatt attaccaaaa
     5941 tcttatatag aaaacttaaa taaaagtcgt agtacaggaa atcgccatgc tgtaagtagc
     6001 tggatacatg tttgggaatc tgtttataca atgttagaaa gcttcccaaa atatctgcca
     6061 aacacttatt taacatacta tcttttaagt aaagaaacag tggaacattc aaacataaat
     6121 cgtactcgtg caaatgaggt tatggaaagc cgtgaaaaag acttatttga aggtgtgaga
     6181 cattacttag aaactggaga aatttctgaa aaagtatttt atgcaggttc acatggagat
     6241 tggattagtg atttggcagt ttcaattaaa aatgatacac gtagtcgttt tttagttata
     6301 actgaaaatc gtggagctat accaaacatg ccatatgatg caatggtaga aattccagca
     6361 tatattggta aagatggtcc agaagttatt gctcgaggtc caatacctct attccaacaa
     6421 ggattaatga tgcaacaact taacagtgaa aaattgatag tagaagctta tatagaaaat
     6481 agttatgaaa aagctttaca agcattcaca ttaaataaaa ctattccaag tatgaatgtt
     6541 gctaaagaaa ttttggatga aatgatagaa gttaataagg aatactggcc agaattaaga
     6601 tagtaggata aaagaaaggg tttttattta ataattagat atgttttcag agtcttttat
     6661 caaaatgtat tcacaaacat atggcaatat attaaaataa agagtgatat ttttagaaga
     6721 atggggtgat gcaatgaaca ttataaataa aattttttgg caagaagata ggattggcat
     6781 gattacaaat aatttagagg cagatacaca tagccactgc atgttacaac tgttcttagg
     6841 gatagaagac agcattgaaa taacagtcaa tgaaaaatta gttaaatgta attgtatcat
     6901 agtagataaa aatatctctc attcattttc agcaagaaaa aaagtttatt actctgctat
     6961 tattgaacct acatctatat atgcagaaca gcttacctca aaaatgaatg attttggcta
     7021 ctggatatgt gacaatgatg gattagagaa attacgtcaa caaggaactt ttttgatata
     7081 taactctagt aaagaacaat atttaagatt tatggaaatg cttaataact atttgaatat
     7141 acctataact ctcaaacatt atgatgacag aattactgaa ttgctcaatt tattacgtac
     7201 ttgtaattgt gataatcata ctatttcaag ttttgcagat aaagtgtccc tatcagcaag
     7261 tagattatca catctgttta aagaacaaat agggatacct ttaaaaagct atatcttatt
     7321 tcatcaaatg gagtgtgctt ttagagaatt actttctggg aaaaatgtta ccgaagcttc
     7381 tatgactgct ggattcgata ctccatcaca ctttgcaggg acagttaagc gaatgatggg
     7441 aatgcctgtt tctttatctt tgaaagatag cgaatttttg aaagtatatt aaatattctc
     7501 gttatataat gaagttgtac taagaaaggg gctttgttat gaataaagaa tatttagagg
     7561 aaacaaaaat gttaaattat aatgaaccac aacttaagct tttggtatca tcaaagaatt
     7621 ggttggaatt agatgatttt cataaaataa aatctattta tgagtttgta cagaatgata
     7681 ttttatttgg atataatgct tctgatatgt taagcgcaac acaggtatta aatgatggta
     7741 tgggacaatg caatacaaaa gctactcttt taatggcatt attacgtgca gtgaatatcc
     7801 cttgtaggct tcatgctttt gatgtaacaa aagattttca acgaggtgct acatccaaat
     7861 tgatttcact attagcacca aaatatattc ttcatacttg ggtagaggta ttttatcaag
     7921 ataggtggat tgctctggag ggtgtcataa ctgataaaaa atatttagaa gcaattcaga
     7981 agaagttttt taatcatggt ggaacgttta aaaagtatgc aatagctaca aatgatttga
     8041 aaaatacatc tattgattgg gatggtaaag atacttttat ccaaaaagaa gctatcgtgt
     8101 atgattatgg tatttttcca tcaccagatg tatttttttc tactcattca caacatatgt
     8161 caaaattaaa aaatttcata tatgtacatc ttatcagaaa aataatgaca aaaaatgtat
     8221 gtaaggctag aaacaattat attgataaaa acgaatgagt gtttaagagg taactatata
     8281 ggaaatatat agaataatta aaactgtact ggttaaaatg aataaaaagg agttatcata
     8341 gagcaaactt aaattgttca taggataact ccttttctgt attaacttct aataatttga
     8401 tgtcttttta taaaacatat agatacaatt aaaccagtaa atgcaagagt agctgctatt
     8461 gaaaatgcaa catctattcc atgaatgtta gacagcatgt ttgaactcat atgagctttc
     8521 ttagtagcac ttgtcattat agtaataagt atagcagaac ctatagctcc agatatctga
     8581 cggagtgtgt tattaattgc tgtggcatga gatataagtt ctctatctaa tgttttaatt
     8641 ccccatgtag ttaatggcat taaaaccatt gaaataccaa tcatacgaat acaatacatc
     8701 aatgacacat gaattaaaga tgtatctctg cctaaaaaag cgaaggataa agttcctaaa
     8761 aacaaacatc ctgttcctaa tattgataga gcatgaggtc catatttatc taaatgacgt
     8821 cctgcaattg gattgagtac aaccatcaat atagcaccag gtaacattaa tgaacctgaa
     8881 gataatgcag aaaatcctct tacagattga acgtacagag gaatcattaa tgtagcagac
     8941 atcattgagg catatacaat cataattaat attgtagaaa taagaaatgg tttattttta
     9001 aaaaccctca attctaaaaa aggttcttta gattttaatt gacgcaatgt aaataaaatt
     9061 aatgacataa ttcctattaa tattggtaaa tatgtggcaa tattagtcca cccataatta
     9121 ccttgattag taaatccaat taataaacca ccaaaaccta gagatgatag tactattgaa
     9181 ggtatatcta actttagttt ttgagtttcc ccaacatttc ttaatagaat aaatgatagt
     9241 ataacatcta atatagcaat aggggaaaga aacagaaata aactatgcca accaaatgaa
     9301 tctactaacc atcctgataa agttggacct attgctggag caaatcctac tgtaatacca
     9361 actaaactca tagctgcacc acgtttttcc tcagggcata aatagagtat aataacttgt
     9421 aaaagtggca ttagagaacc agaacccatt gcctgcaaca cacgagcaat caacatagtt
     9481 gaaaaattat tagaaaaaag tgcaataata catcctatag aaaagataga cattgatgta
     9541 ataaataact ttctagtact aaatcgattt attaaataag ctgttgtggg tacaataata
     9601 cctgcaataa gtagataaat tgttgttaac cactgaccaa tatcagcagt aattttaaaa
     9661 tctgacatta tattaggtaa tgctgttgac aatagtgttt gactgagaga acttataaaa
     9721 gcaccagtca ttacaattgc tattattaaa tttccttttt tattgttact ttgggtttcc
     9781 ataatttata acgtctcctt ttttaattga tttttaatgc tgttcaataa aagtactttt
     9841 ggtcttaaag tgtcttcttt ttctaaatat gcaatcattg catccataat taaagaatca
     9901 tattgttgat tttttccctc gacgtataat ttttttattg cttctagtaa tttaggattt
     9961 tcaaggagag aagtatcagt tttgattagt tcttctatat cttgtatcgt tttaaaaaat
    10021 agcgatttat tatgaaggga aaagtaaact tcatcttctt gtttatgtaa aaacattgat
    10081 ttttcaatta aaggatttag attatccata ttttctacaa ctaaatctag aatttgtata
    10141 gtaaatttgg ca
//
LOCUS       DAEPLF010000124        10122 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEPLF010000124 DAEPLF010000000
VERSION     DAEPLF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.124"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..675)
                     /locus_tag="KR314_002498"
     CDS             complement(<1..675)
                     /locus_tag="KR314_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0328873.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYS"
     gene            complement(676..1551)
                     /locus_tag="KR314_002499"
     CDS             complement(676..1551)
                     /locus_tag="KR314_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328874.1"
                     /translation="MRSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(1555..1923)
                     /locus_tag="KR314_002500"
     CDS             complement(1555..1923)
                     /locus_tag="KR314_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0328875.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(2106..2765)
                     /gene="deoC"
                     /locus_tag="KR314_002501"
     CDS             complement(2106..2765)
                     /gene="deoC"
                     /locus_tag="KR314_002501"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBH0328876.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(2801..4345)
                     /gene="murJ"
                     /locus_tag="KR314_002502"
     CDS             complement(2801..4345)
                     /gene="murJ"
                     /locus_tag="KR314_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBH0328877.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILITGIIFIGITSVMSAFLQIKENFIVVGF
                     GSIPYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKD
                     DSLIKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYSDRLNGFVTGTFTA
                     SIVSVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFER
                     GAFDARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVL
                     DLVLIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIM
                     GVLSYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKR
                     KLHLKK"
     gene            complement(4819..5703)
                     /locus_tag="KR314_002503"
     CDS             complement(4819..5703)
                     /locus_tag="KR314_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0328878.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(5994..6470)
                     /locus_tag="KR314_002504"
     CDS             complement(5994..6470)
                     /locus_tag="KR314_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBH0328879.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGVLDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQAKDLEEHHHHDKNCGCGCNHHHEN"
     gene            6737..7912
                     /locus_tag="KR314_002505"
     CDS             6737..7912
                     /locus_tag="KR314_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBH0328880.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(7993..8796)
                     /gene="proC"
                     /locus_tag="KR314_002506"
     CDS             complement(7993..8796)
                     /gene="proC"
                     /locus_tag="KR314_002506"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBH0328881.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINNEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(8836..9420)
                     /locus_tag="KR314_002507"
     CDS             complement(8836..9420)
                     /locus_tag="KR314_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0328882.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         3401 a         1697 c         1060 g         3964 t
ORIGIN      
        1 tgaataaata attttttcta aatacaatag cattttatgc atatttttag ttgcaaaatt
       61 aactatataa attaccatta atatcggtaa tcctatcaat atagtaatac tagtaacttt
      121 tctaactcta tatgtaaaag ctccaagtaa tactcctata aaacctatac atatttcatt
      181 tatacatatt tgtacaaaat aatttaaata agttagtggc atacttgatg ttgaaggatc
      241 tatcagtgct gatatagatg tccaaatcat atctctcttt agtaaaactg atagttctat
      301 actgtaacct gttataattt ctataaataa cttcgataaa aaaatcaaaa ctatacctaa
      361 aatagaaatc acaaagctta aaataattaa atctatgaca atagctttta tacaactttt
      421 tcgatctgct cttatactta aagctcctga aaagtcttta tttgctatat ttactccata
      481 cacaaatagc agtatagtta tatatgttaa aataggattc acaatcgtgg ataaattagt
      541 tttagatgct tctccaaaaa tcttgaggat aaacactggc aaaatattca taacaattcc
      601 cacaatcata aatataatta tatacaattt agtattttta ttaaaaaatt ttatatatgg
      661 ctttaattca ttcaattaca attcctcctt cttagtcatg ttaacaaaca tatcttgtag
      721 ccctatgtat cctacatcaa tatttgaatt tttaagtatt ttttcatcat tttcactaaa
      781 atcaccatag taaaaataag caactgcatt tccaaatgcc tttttaggtt ttatattttt
      841 tatacattct aatttttcta actcttcttt atttccagta atataatgag ctttttgttt
      901 tatgacatca atttcttcat ctataataat ttttccctca tttaatatta ttacatgctc
      961 cagtaaatca tctatttcat caatcaaatg agttgatatt attattgtcc ttggattttt
     1021 tatataacta tctaaaatta tgttataaaa ctcttgtcta tttactgcat ccaaaccaat
     1081 tgttggctca tcaaatatgg tgatagctga attagaacaa atacctatta tatttgaaag
     1141 tatagttttc atacctcttg acagttgttt atactttttt ttagtattta aatcaaaata
     1201 tttgcataat tcttcttcta agcctttatc ataactttta taaaaactag aagaaaattc
     1261 aaatatctga cctactttaa aatctggact ataaaattct ttttccctga cgatacaaac
     1321 ttcttccaaa acttttatat cttcttttgg gttctttcct aatatttcta tctcaccttc
     1381 attttgaatt aactgattta ccattatatt taataatgtt gtttttccag ctccattctt
     1441 acctaataaa ccatatatct tatttttttc aaagtttaga gacatattat taaaaatatt
     1501 ttgcttctta aaactgtgag ataaattttc tatcttaata gaactcctca tatattaatc
     1561 ctccttgaaa ttaattataa tatcaaccaa ttcttctcta tttatttcta atttgttagc
     1621 ttcttgaagt aggtttttta caaaattatg tttaaaattt tcttttctag cttcttttat
     1681 aatagttttt gcaccatctg atacaaacat ccctatcccc ctcttcttat ataaaatatt
     1741 tttatcaacc aatatattta taccttttaa tactgtagct ggattaattt tataaagctt
     1801 tgaaagctct gttgttgatg gaacctgttc ttcttctttt attccatttg atagtatctc
     1861 atcttcaatt gctcgtgcta tctgaataaa tataggttct tcattattta atactaattt
     1921 cataggttcc tcctttctcc attagttagt tactcaacta attaactata taacttataa
     1981 tatcttgaaa aatattttct gtcaatacta aataaaaaac agagggaaaa ttccctctgt
     2041 tttttgttct ttatatttat aattaaattt tgatttttgt aatcctatcc cataagttaa
     2101 aaactttaat aatctgttgt atcttcattt tttaatattt tacatattga gctagttcct
     2161 aacctttctg ctcctgcctc tataaatttt tctgcatcag aaattgattt aactccccct
     2221 gcagctttta tttttacatt ttttccaaca tgctctttca taagctttat gtcttctaaa
     2281 gtagctccac ctgttcccat tccagtagat gtttttataa aatctgaacc tgacattgtt
     2341 actatttcac acatttttat tttttcatct tcatctaaat aacatgtctc tataatgact
     2401 tttaatactt tatctcctat agcttttttt atttctttta tttcttttcc tatattatca
     2461 taatctttat tttttatatc tgaaatgttt acaaccatat caacttcatc tgctccattt
     2521 tcaatagcat cttttgcttc aaatactttt gtagcagtag tttgatatcc caaagggaac
     2581 cctatcactg tacatatctt tattttacct tttagatatt ctgatgctct tttcacatat
     2641 gatggtggta tgcaaactga tgctacactc atatctacag cttcatcaca taaaatttta
     2701 atatcttccc atgtagttgt agcttttaaa atagtatggt ctactgtttt taatatatgt
     2761 ttcataaaat cctcctaaat ttataattaa tataattaga ttactttttt aaatgcaatt
     2821 ttctctttgc aatattcagt atcatatcaa cctcttcaac tttaaaaata gtcattagta
     2881 gtgtataaat tccacctcca actataactg aaatagctag tgatacaaac tcattaaatg
     2941 ttcctactcc caaaattcca aatataaatt tatacgtaaa gtatgataaa acacccataa
     3001 ttaaagatgc aactaccgat ttcaaagttg cttttattat tttatcttgt ccaaaatatc
     3061 caacttttct ctttaggttc aagaagagta acaatataca tgcaattgat gatgaactag
     3121 ttgcaagagc aagaccacca tgagccattg gttttattaa aactaagtcc aatactatat
     3181 ttacccctac agatataatc ccatttacca taggtgtttt tgtgtcttgt aatgaataaa
     3241 aaacctttcc caaaatatct cttaatccaa atgcagtcat accaactgca taaaaaatta
     3301 aggcagttgc tgtcatttgg gttgccctcg catcaaaagc acctctttca aaaatgattc
     3361 taacaacagg agttgcaaaa aaaatagaag caactgaaat aggaatcata gaaattatta
     3421 tcatatttat actagattta actgaactag taaacttctt ttgattattt tctgcactta
     3481 acttagataa cataggatac ataacagata ctattgaagc tgtaaatgtc cctgtaacaa
     3541 aaccatttaa tctatctgaa taagtaagtg ctggaatgct tcctttaact aatgttgagg
     3601 ctaaagttgt atctaccaat gagttcactt gattaacagc aacacctata aatactggtg
     3661 aaagtaatgc tagtaattta ataaggctat catctttaaa gtttaagtag tataagtatt
     3721 tgtagttcgt ttttttaaca aaaaacatgt aaaagagcag ttgaactacc atagcaacca
     3781 cagcaccaat tggaagtatg tatggtccaa atacggtact taacattatt gaaataatta
     3841 ttactatatt atagggtata ctcccaaatc caactacaat aaagttctct ttaatttgca
     3901 gaaatgctga cataacactc gtaataccta tgaatattat tcctgttatc aatattttag
     3961 taaattttac ggtaagtaaa aacctctctc cttcaaatcc tatagcaaat atacttacaa
     4021 gttgctttga gaaaataact cctaatatag ctacaactat gcatatacca actattatat
     4081 ttaaaacatt gtttaaaaac tttagtgctt gcttttcacc ttgtttacta cttatatctt
     4141 gatacattgg aataaaagta gtaactatag cagtacctat tgctgcaaat ataatatttg
     4201 gtatattcat agctgttaaa taactctctg tatataagcc tgtaccataa attgatgata
     4261 atactaattc tctacccatt ccaagaatct tagagattat agtaactatc atcaaataaa
     4321 aagttgcttt agctaccttg ctcattttat cactccactt ctaacatacc tctttttaat
     4381 caagtaaaat ttggctcatt agatataatt ctatcaacta atttgccttg cttagcccta
     4441 tcaaaatata attaacacct atttcttaat atatcacaaa ctacattatt cttcatattt
     4501 ttttatttct ccaataagtt ttacatcttt cctatataaa ttgtatagct tcaattatat
     4561 tcaaagaaat ggagttgcta cccgataact ttaaaagtaa aaaacagatt ttattatctt
     4621 tttaaactat attctaacat attttgtcca tattgagaaa ttttactaaa ctattatgta
     4681 ctttattaaa taaataaaat aacagcacac ctataaaata caagtgtgct gttcataaac
     4741 agattttata aaaaaataat tcctaagtag agattaacat gctttctcat actctcatat
     4801 ataatgtgtc taaaaatatt aataaaaatg ctttaattgt tttaacttac ttggatgtct
     4861 taattttctt attgctttag cttctatctg tcttattcgt tctctggtaa ctccaaatac
     4921 ctttccaatc tgttctagtg ttttagggtc atcatcatca attccaaatc tcattcttaa
     4981 aacttgctgt tcttgctcac caagacctgt aagtagttct tctatctttt cttttaaatt
     5041 atgctgttca acttcgtgta ttacaacatc tttaaaatca gcatctgatg gtataaattc
     5101 aactaaacta ctatcctcat cttcttttaa tggagtatcc aaagatacaa catttttatc
     5161 tcttcttaat aattccaaca ctttgtcaac ttctaagcca catgcatctg caatctcatc
     5221 tatagttggc tctcttaata atacatttaa aagttcttgt ttctttttct ttacaaaatt
     5281 tattctctga tgaagatgta taggtatcct aatagtattt tcgcaatcgt cgatatatct
     5341 tgttatactt tgttttatcc accaagttgc ataagtgcta aatttgtatc cttttttata
     5401 atcgtacttt tctacagctt ttataagtcc tatatttcct gcttgaatta aatccaacat
     5461 atcaatacta tctcttttat atctctttgc tatgctcact accaacctat aatttgaatt
     5521 tataaatttt tcttttgcta ctgaagatga gttgattatt tccttagcca gctctacctc
     5581 ttctcctgct gacaacattt tgtattcctc tatttccttt aaatacattt tcatcgcatt
     5641 tgatacattt gatgaaccca cacaaatata attttcataa ttattttctt ttttagtatc
     5701 cataatcaaa ccccttctat tcatagaatt tgctaaaaca aaaataaata atttaaacct
     5761 acataatcta caattacata atatcccatt ctttaaatga attgttttgt atattatgta
     5821 tacaaattta taaaaactat acctataata ttattctact tctaaccgca atatcctcct
     5881 tatatatata atttatttta ttatatgttg ttatattctt agttttttga atgatatatg
     5941 tctaaaaaaa gactgtttac taaacagtct ttttttatat atatgctcaa tatttaattt
     6001 tcatgatgat gattacatcc acatccacaa tttttatcat ggtgatgatg ttcttctaaa
     6061 tcttttgcct gttgttccat ttcatgaaac tcttttacca ttatatcata agttggaaga
     6121 gctgcctcag tatcgtattc ttcccctctc caatcagcgt acatcatagc atccccatta
     6181 gttaaaacca atctacctga catagttata ttatccactt cttctaaaaa ttctatttgt
     6241 ttttgtaaaa cttctccttc taactctaca tcttcatcta aacattgcac tatcattaca
     6301 ggtgcataat aatcttcttc atctttaaca tttacaacaa tatctaaaac tccttctgca
     6361 ccttcttcaa attctgccaa ctcaacattt gtatcaatca tatcttgagg gactaataaa
     6421 tcttctatta aatattttat tactctatct tttactaatt cttttgacat ttatttttct
     6481 ccttccgatt ctataacatt atcataatca tttctatatt atagtatact acattaattg
     6541 ttttttcaat ctcttacaaa aattattttt ttattatctt ccttatattc ccttttttat
     6601 tacaaagttg taatcgcatt gatttaaaaa atagtttata tttagtataa tagaaatgta
     6661 gcacatattt ttattcaata tattaataaa aaaatagatt gaaaatgtaa tgtatgttaa
     6721 atctaggagg aatattatga agtttaaaaa acaaattagt tctattgttg tgtctactat
     6781 gcttgtactt gggagtatga acctttctta tgcagatggt acaaatgtag ttacacttgg
     6841 agctaactta tcagagtctc aaaagcagca aatgcttaat tactttggag taaaaaaaga
     6901 ccaagtctta gttttagatg taacaaatgc agaagaaaga cagtatttac aaggtgttgc
     6961 aacagagggt caacttggaa aagttactat ctcttgctca tacgttgaac ctactaaaaa
     7021 aggaaatgga attaatgtta aaacagcaaa cttaacttgg gtaactagtt caatgattgc
     7081 ttcaaccctt acaacagctg gtcttgaaga tgcaaatgtt attgctgctg ctccatatcc
     7141 tgtaagtggt actggagcat tgacaggaat catgaaagca tttgaggatg cttcaggaaa
     7201 gaaacttgat gaaactaaga aagaaattgc ttctgaagaa ttggtagtta caggtgactt
     7261 aggagatgat atagggcaag aaaaagcaac aggtgtaatg aatgatataa aaacagaaat
     7321 tgtaaagaat ggtacaaaag ataccaatca aatagcaaat acaataaata atgttgtaaa
     7381 taattacaat attactatta caccagaaca aaaagaacaa attaaaggtg taatgaaaaa
     7441 aatagctgac caaaattatg attacaatag catgaaaaat acactagata atgtatcaga
     7501 taatgtaaat gaacaattaa agaaattagg tgaaagtgtt aagggttctg gtatactaga
     7561 tactattggt ggatggttta gtggaatagg agactggttc tctggtttat tctcaggcgg
     7621 agataaaaaa gatttgggaa tcttaaataa cactaatgat gagtctcttg gctctgatgc
     7681 tgtaataaat tctacagaag gtgttcaaat aaatccacaa aataatacta attctaacaa
     7741 tgatagtgat acaaatagct caaaaaatga tacttcaagt aataatgaaa ataacgatag
     7801 ttctcaaaca aatgaaaaca acacaacaaa taattcagaa aatcaatcaa ataataatga
     7861 ctcagttggt ttctttgaaa aaataaaaaa ttggctttca ggcttatttt agaaattaaa
     7921 caaataaaaa gaattgccta aaatggaaat tttctataaa aggcaattct ctttatttac
     7981 aataaatttt tattactcac tcattttttt agatttttct atacatgcac atattgcttt
     8041 tatcacagaa gctctaaaac cttcttcttc aagtacttta actgcttcta tagtagttcc
     8101 accaggtgaa cataccatat cttttaattc accaggatgt tttcctgtct caagtaccat
     8161 ttttgcagaa cccataacag cctgtgatgc aaacttatat gcttgtggtc ttggcattcc
     8221 tgcaattact gctgcatctg ctatagcttc tataaataaa aatacatatg ctggtgcaga
     8281 accacttgca ccaattacag cttctataag atactctggt actacctcag tatttccaaa
     8341 agaattaaat acttctgtaa ctgcttctaa gtcttcattg tttatatttt tatttataga
     8401 aagagaagac atggcttcat ttacaagagc tggagtattt ggcatagttc taactatttt
     8461 tttatcttct cctataattg actctatatc ctttatagat tttcctgctg ctattgtaac
     8521 tataatttta ttgtcatcta taaagtcttt tatctcttcc aaaatatcat catatatatc
     8581 tggtttaaca gctacaatta ctatatcaga ggttctagtt acttccttcg agtcttttgt
     8641 agtatttatt ccaaaatttg catctattct atcgagagtt ccttgactat atgctgatgc
     8701 tataaccata ttaggttcaa ctaattttga atttactatt ccccctatca tagcgcttgc
     8761 catatttcct gcacctatga atccaatttt tttcacttta gtacctcctt attgactaat
     8821 agtaatcgtt aaaatttaag cttctttaac tgcatcagac ttttcaaatg gatttggtat
     8881 acttactacc aaaattttca ctaccttatt agtatagttg acccaattat gattgttctt
     8941 ttcactatta tagtgtattg tgtctcctgg atacatttct atttgttcat ctcccaaaaa
     9001 cactgtaaga gtaccctcta atacatacac aaattcctca ccttcatgta cgtaacaaca
     9061 aatttcctca ctactcttac ttggaagaat ttcaattaat cttggtagca ttgttttttc
     9121 ctttaaacta gatgataaat gataatgtat aaatgttgag ttttctacat caaatacttc
     9181 tttttcataa cttcttaata cagctctttt atgctctttt ggcttcataa aaaagtaagt
     9241 taattctaca tctagtacac ttgctatttt tcctaaggaa tctgttgcca cacttgttaa
     9301 tcctctttca agttgtgata aaaaaccaat tgacaagttt gttttttcac tcatatcctt
     9361 cagcgtcatt tgcttttgag ttctgagttg ctttattttt gctcctatgt ccttattcaa
     9421 aaccatcacc cctaaataat tttaattttt tcataatata aatacaaaaa tataaatata
     9481 ggtactgaac ctatctttat ttcatttaaa aaaaattttt ataagtatta ctataattta
     9541 tcaatctttt tttgtctaca tttttacgat tatttacgat ttatttttca catatgtata
     9601 tttaatcttg ccaactaaag atacattttt tataagcatt tactctttat ataataaaac
     9661 ttaagttgtt catgtagtct tcttctggtt taattattaa aatttcaaat tatattatta
     9721 tttctacaca ttattacatc ctttagtaca attataacat aattttacaa tttaatgata
     9781 tctattcaat gtcttttttt actctacaac atattttatt ttagagttta atactatata
     9841 tattttttat atacagcctt gtcatttttt agattgtcaa gagttattga agtattttat
     9901 gtagatttat attttatttt ctaatttctc atttaacaag aattgcttat gataaaaaat
     9961 aatatcatct caataagatt cttgtaaata tatctatatt ctaaatataa tttctttttt
    10021 tgtcaaaatt tcttcattct ttaataatta atttattact tataaaataa actaattatc
    10081 tatacaaatt ttataatttt taataaaata cacaaaaaaa tt
//
LOCUS       DAEPLF010000125        10114 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEPLF010000125 DAEPLF010000000
VERSION     DAEPLF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10114
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.125"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..817
                     /locus_tag="KR314_002508"
     CDS             <1..817
                     /locus_tag="KR314_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBH0328883.1"
                     /translation="NKIRKIFKLYALEISKNFERDCLNSFQNLKQISDRGLSLGEKYI
                     EESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRLFNNLNKTFIIPNKNKKNGHA
                     SFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITAVHSYIDEESIKKSNALFNNY
                     KDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDFSKEIERLTEFLGYDIKEYKA
                     SLMDIYINELMESDENDVENAKEKIEKYARYIGCSDENLYVARVNAIYTFANA"
     gene            1178..2476
                     /locus_tag="KR314_002509"
     CDS             1178..2476
                     /locus_tag="KR314_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBH0328884.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSTERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKLLSSDLNN"
     gene            2666..4057
                     /locus_tag="KR314_002510"
     CDS             2666..4057
                     /locus_tag="KR314_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBH0328885.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            4234..4821
                     /locus_tag="KR314_002511"
     CDS             4234..4821
                     /locus_tag="KR314_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0328886.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            4854..5645
                     /locus_tag="KR314_002512"
     CDS             4854..5645
                     /locus_tag="KR314_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0328887.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(5826..8243)
                     /locus_tag="KR314_002513"
     CDS             complement(5826..8243)
                     /locus_tag="KR314_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0328888.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNLSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKTISLQGNILSIIFIPLGAI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKQAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     SYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLIRNIEKKSL
                     TERLRSQ"
     gene            complement(8256..8921)
                     /locus_tag="KR314_002514"
     CDS             complement(8256..8921)
                     /locus_tag="KR314_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328889.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGSLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(9003..10034)
                     /locus_tag="KR314_002515"
     CDS             complement(9003..10034)
                     /locus_tag="KR314_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0328890.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFNISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDLSHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
BASE COUNT         3701 a         1222 c         1521 g         3670 t
ORIGIN      
        1 taataagata aggaaaatct ttaaattata tgctttagaa atatctaaaa actttgagag
       61 agattgttta aatagttttc aaaatctaaa gcaaatatct gatagaggtt taagtttagg
      121 agaaaaatat atagaagagt cttttaagaa aggaattgag actatagtaa gttttggaat
      181 tataactata gatatcgata cctttaaaag cgtttattgt gaaaaatatt tagattttaa
      241 gagacttttt aataacttga ataaaacttt tattatacca aataaaaata aaaaaaatgg
      301 gcacgctagc ttcttagaaa tagaatcgac aataaagaaa atgagcaatt atttatacaa
      361 tgattgtttt aaaattcatt atgctgttgt agatgccctt atagaaaatc gtataactgc
      421 ggtacattct tatattgatg aagaatctat aaaaaaatct aatgccttat ttaataatta
      481 taaagatggt tttattggga aggctgacga atgcaaagta gtaaaacaaa taataacttt
      541 aaatccatat agggaagatg tatacgaatt tttggtaaaa gaagatggag attttagtaa
      601 ggaaattgag agattgactg aatttttagg ctatgatata aaagaatata aagcgtcatt
      661 aatggatatt tatataaatg agttaatgga gagcgatgaa aatgatgttg aaaatgcaaa
      721 agaaaagatt gaaaaatatg ctagatatat tggatgtagt gatgagaatt tatatgtagc
      781 aagagttaat gccatatata cttttgcaaa tgcataattt tattaaacat taaatattag
      841 atgttcaatt atataaattt aaataaaaaa atatccttat taataacaaa tagtttatat
      901 taaaaaccat tcttaaaatg agtggttttt tattttgaat agatatattt taaacctaat
      961 agcattccta tctttaatta gccaaaaatc aactggattt catatagact acataatttt
     1021 aaattactaa aaaagacaat atattgacaa aaatgaaata tgttgataaa atgaaatagt
     1081 caatatggtg tcttaattta aaaatttatt tatgttgtat tagataaagt tgttttatat
     1141 ataaagcata aataattatt ataaggagaa atagattatg aaagtaaatg tatacaatgt
     1201 agaatcattt gtggatgaaa gacttaagat tatggtttca gacttaattc caaatactaa
     1261 acttaaaata agtgtgaaaa tggaatttcc atggtgcaag ggagaagaat tttcatctta
     1321 tggagtgttt tgttcaaatg aaaaaggaga agtagattta gatttagttg aacctataga
     1381 aggtacttat aaggctgata attgtatggg gttaatttat tcgctggaaa aatctaaaac
     1441 tgaaggtaaa aattttgcag aaaatatatc tattgataaa ccaataatta tgaatatgat
     1501 atttgaaact tctactgaaa gaaaagaagt tgaattaaag agaatattta aatcagaaga
     1561 tattaccaca aaatacatat ctgatgagtt tataggaaaa ttattttata aacaaaattc
     1621 taataataaa acaattttaa tgctaggtgg ttctgatgga aatttagacg ctctagacct
     1681 tttagcagct ccacttgcat ctagaggatt caatgtacta acagttgcat actttgcttt
     1741 agaagggtta ccagataaat tagaagaggt tccattagaa tattttgaaa aagtatttga
     1801 gtggattagt gaaaatgaaa ttaccagtac taaagaaata tttgtacatg ggacatcaaa
     1861 gggtggagaa ttggcctttt tacttgcttc aagatatgaa caaattaaaa aggttgtagt
     1921 atcacaacct cacatttatt gtttccaagc tcttaatgga ttgatgagtg gaaatgatac
     1981 ttcatcatgg tcatataaag gagagccatt tccatatatc aaagtagaca atgatatatt
     2041 ctttgaagaa cagaaaaaaa atatatcaaa aggtattcca tttggattta atagtactta
     2101 taagaaaagt cttaaaagag cagagaatag aaaagaggct agaataaaaa tagaaaattc
     2161 caaagcagat atacttatga ttgctggaaa agaagataac atatggaata gttatgaagc
     2221 ttgtttagag gcattagaaa tctttgaaga aaataattat ccatataacg tagaattatt
     2281 aacatacaat aatatgggtc atccgttacc aattccatat attatgcctc taaaggagac
     2341 tttatgtatg agtatgagtg gagggatttt ttcttcaggt ggaactgtag aaggaaattc
     2401 aaaaggacag tttgaatcat ggaatagaac tattgaattt tataaaaaac ttttatcaag
     2461 tgatttaaat aactaatatt aagtgattta agtaactaaa aatgaagcgg tttttctata
     2521 tatgcacaaa atctatcaat tttatagatt taatttattg tttatataat ttgcatgtta
     2581 tatactaatt ttaatgacat tgacaattta taaacaatta taaagttgta aacttataag
     2641 agttattgaa aggtggagtg aagttatgga aagactcgct agaagggaac aacctcatat
     2701 aaaatttgga atgtttaaga taagaattcc atttatacat tacagatttg agaaaccaga
     2761 agctattcaa ggtataatat caagtatgac aagtttaggc acaattggcc ttagtacaca
     2821 aattttggga ttaccctatg aagtggcatg gagtatggct attattaact caatattata
     2881 ctgtttgcat gtttttatgg gagacccagt tgtacctggc tggattacag cttctataac
     2941 tcttacaact gcatatttgt taaagttcag catgggcatt gaaagaatac aagcattaac
     3001 agctttacaa atagatttag gaataatatt tattttaatg ggtataactg gtgtagctgg
     3061 aaagttagtt agtaagatac caaattctat taaaggtgga atactaatgg gtgtcggtat
     3121 atcgactata atatcagagt ttaatcctaa agggagattt gattcttatc ctatatctat
     3181 aacagtagga attttggttg cttgttttgt aatgttttct gaaagatttg atactctaaa
     3241 agttaagaat aaatttttat ttcatttagg agaatatggt gtagtatcag ctattttagt
     3301 gagtttaata gtaggtatat ttagtaaaga aatagaaatt cctagattta gttttgacaa
     3361 tttagtttac atagtagatt ttaaaaatct gataaataca gtatctcctt ttggaatagg
     3421 ttttccaagt gtaatgttat ttatacaagg gataccaatg gcttttatga tatacataat
     3481 tgcctttggt gattttataa caggtgaaaa cttagtactt tctgaaagtg aaaatagaaa
     3541 agatgaatat atagatttca attcaaatag gtctaatgtt ataagtggga taagaaatat
     3601 ttttatggca atcttttctc catatatacc aatgtgcggt ccattagctg cgacacttac
     3661 aggaagtgtt gctcaaaggt acaaggttgg aaaggaagcc atgcagtctg tttttagtgg
     3721 catgggtaca ttagtatggg tttcagcaat ttttatctgt ttttatccaa ttgcgcagat
     3781 tgctacacct cttataccat tagcattgtc agtaacactt ttagttcaag gatatttatg
     3841 tacaaagcta tcaatggagc tatgtaatac aggtgttgag aagggattga ttggacttat
     3901 gggaggtgta attgctgcta aaggcggaac atggggactt gctgtaggtt ttatactgta
     3961 tttcatttta attgattcta aaaaaagaaa agaaaaagat attaattcaa tagaagattg
     4021 tgtatatgaa atattagaca aaaagaagag tatttaaagg aaaatatgag ttgctgttat
     4081 ctattaatag attaattgca actcatgttt tatttataag ttttaggaat tgcaaaaaaa
     4141 tgcaaagctt tttcttgaca taatttcttg atatgttact atagttatat aacatatgtg
     4201 ttttataaaa atttaatgga gaggtgaaca tatatgccac ctaaagtaaa gataacaaaa
     4261 gaaatgattt tgaatacagt cttggatatt acaagagaag tgggatttga agctgtaaat
     4321 gcgaggagta ttgcaagtaa attacagtgt tccactcgac caatttttac ttgttacgaa
     4381 aacatgaatg aattaaaaaa tgaatttctt gcttttgcat atgaatatta taatcagtat
     4441 gttgttaatt atcgtaattc taaaagtgta agcccttatt tactgcttcc gctttcatac
     4501 attgaatttg ctcaagaaga aacgtattta tttaagttgt tatttataaa tgatttagat
     4561 ttgatgatga aagaggcgaa tgatttttat aaagaaattg acaatgaaaa aaaggcaaga
     4621 cttttttcgg aaagcattgg aatagagttg gaatgtgcaa aagtaatatt tttagattta
     4681 tttctttata ctcatggcat agctgtctta acagcaacaa aaaaattaac attagataga
     4741 tatagtgctg aaaaaatgtt gaaaaatata ttaacagctt ttataagaca agaaaaacca
     4801 gattggaatt tgtctatttg atgtttatta actaatatag aagaggtgag attatggaaa
     4861 caaaacaata cttaattgac ttttataata cttatgatga agatagtcga cttgcattaa
     4921 aacatggaat ggtggaattt ctcactacta tgcattacat tgataagtac ataaaatcag
     4981 gtgattgtgt gcttgaaatt ggcgctgcaa ctggtcgtta ttctcacaca ctggcacgtc
     5041 agggatatga tgttgacgca gtggaattag tcgaacataa tatagaggtt ttccataaaa
     5101 atactcaatc aaatgaaaat ataagcatta ctcagggtaa tgctatggat ttatctgttt
     5161 tcccagataa taaatatgat attacgttac tgttaggacc actatatcat ctttataaca
     5221 aagaagataa acaacaagct ttacatgagg caattcgtgt aacaaaacct ggtggtgtgg
     5281 tttttgctgc atacgttata tctgatggct gtctcattga cgaggggttc catagaggaa
     5341 atattaatgt atctgaatat attgaaaaag gtttgattga tccacagaca tttgctgcta
     5401 agtctgaacc aaaggatttg tttgagcttg tacgtaaaga aaacattgat gatttgatgt
     5461 ctgcatttaa tgtaactaga ctgcattatg tcgcatcaga tggtttggct ttgtatatgc
     5521 gtgaagcagt agactctatg gatgatgatg catttgcgtt atatttaaaa tatcaccttg
     5581 ctacttgtga acgtgaagat ttagtaggag ttacaagtca tgcaattgac atatttagaa
     5641 aataaggaat atgcttgttt atgtctctgg tgatgttgct tggagatata aggaagtgta
     5701 tctgtataaa ataaaaacaa gtagagggac agaaaattat gtaaagtcaa aattttctat
     5761 ccttttagct tttaaaaaat tatgttaaaa cttgtataaa aaatggttat tttttatata
     5821 cattcttact gagaacgtaa gcgttctgta agtgattttt tttctatatt tcttataagt
     5881 aattcagtca ctaaaacttg tataccaatt aatacgaatg aaaaaattaa aatagtaaat
     5941 atagaaaatt taaaattaat aaagtctaat ctaattacat cttttataaa agtgcaaagt
     6001 agatttgaac caaaataacc tagtatactt gaagtacata tagctttgat acttatgttt
     6061 agaccttctc tataaagcat atttcgcagt tgctttcttg tcattccaac ggcttgaagt
     6121 aaggcaaact ctttttttct aattaaaacg ctgtttatta gggtattggt catatttagt
     6181 ataccaaagc aagaaattaa accaacaaaa atgtatgaag ctataattat atattgaaat
     6241 gctctagtaa tcattctata atcatcttca tatgaataaa gcattaaatt tgaatttttc
     6301 tgaattatac ttaacaactt ttcttcaact tctttagaat gctttttatc tattaaaata
     6361 gaaaaatcta aatttgtatt atatggtgtt agagattcta aagttttgtc tgaagcaaaa
     6421 aacatacctc ctgtattgga attgtcaatg attcctataa ttttcatttt ctttttaata
     6481 atatcatttc cactaaagat attaaatgta actgtatctc caacttttaa atttaatcca
     6541 taagattttg ttctatattt atttataatt aaactatctg aatctaaatg tgtatagtct
     6601 atttttccac tgacaatata ttcttcaagt gattttgaaa gttcaggcgt aataccttta
     6661 attagagtaa taaattgttt ttcgtctcta tctttgatgt aaggttctat aatattactt
     6721 ttaattgatt tggattcaat taattttttt actccatgaa tagatttaat ttcattctta
     6781 agagaatctg ttagtggatt attttgttgg atttctgtaa tatgctcata taagttatca
     6841 ctcttaatgc taattatata atcttcgtta tagacaaatt gttgagatag ttgttttttg
     6901 aaatctattg aattagcaag attagcgatt gaaataaata aaactccact aatgcttagc
     6961 gatattaatg caatcaaatt tttctttcta tttgactcga tattctttaa tgctagattt
     7021 tttatattta tataactaaa attttttgct tgttttctaa atttagtttc tgtatattgc
     7081 atggcttcaa tagcagatat tttagaaata attctagctg gtttaaataa agaaattcta
     7141 acagttaaaa acattaccag tgataaaatt aaaattataa ataaatcagc tatcatttgc
     7201 catgctaatg gttgaattat gtaagcaatt atacaagaag ctatagcccc taatggaata
     7261 aaaataattg acaaaatatt tccttgtaga gaaatagtct tttttaactg aaaagaagtc
     7321 atacctaacg atttcaactt agcatacatc tgaactgaat taacaactga aatataaaaa
     7381 atactatata taacaaaaac gctagaaaaa agaacaacta ttccaactac taaaactgta
     7441 ataatctgct caccatcaat taaatttgat tcaatatagt tatcatttaa gtatatatta
     7501 tatttcttaa tacctaaaga taaaccaatt ttctttaact gtgtcttaat tgaagctgaa
     7561 tctttctctg tattttcttc tgatagttta actaatacat ctacagtatc aggtcttgac
     7621 tcgtcaaaag tattactttt attttgtaaa ggtgtagaag ctatattctt aaaataatca
     7681 tttgaaacga ctgtatagta ttgcttggca ttatcttgat aataattttc tagaaatcca
     7741 acaataataa aatcacattg aacttcttta ttcgtaatat catcataata atttaattta
     7801 atgctatctc caatatcttt atctaaacca aattcctgta aatatttttc tgaaatagca
     7861 atttcatttt tatttgtagg tagtttgcct ttcaatggtt gaaaattcat aagtttaatt
     7921 gctgtatcat ctgcataaac aatcgacata atacagtctg tctttttatt acttccaatt
     7981 aatttgtagg ttcctacgcc actaaggttt ttatctttat atagtatatt ttttgctttt
     8041 tcatctacag atttatatat agcatgatat ggagatgcat taactaaatc tacagaagca
     8101 ttatatgaaa ctaaggtcac tgttaataat aaacatgtag aaatcattat aatgatacct
     8161 gaaagatatg ttttaagttt attaattttt aaatcattta aagctatttt tcttataaat
     8221 gtgcgattat tatttctaaa catatactac acctcctagt taacaatttt tccatcttca
     8281 atacgaataa ttctatcagt tagttgtgca atttcattat tgtgtgtaat cattacaatt
     8341 gtttggttga attttttact tgtcacttta agtaaactca gtacatctga actagttcta
     8401 ctatcaaggc tacctgtggg ttcatcagct agtataatag ctggttttgt tacaagtgct
     8461 ctagctatag caacacgttg ctgttgacca ccagaaagat tacttggcat atcatataat
     8521 ttttcttcaa gagaaagcat atttataatt tcatttataa aatccttatc tatggtctct
     8581 ccatccaatc ctattggtaa gataatattt tcatatacat taagtaaagg aacaagatta
     8641 taattttgaa atataaaacc tatatttctg cgtctaaaga ttgtaagctg ttcatcagtc
     8701 atagtagata attccttacc ctttataata acctttccag atgtaggatt atctaatcct
     8761 cccatcatat ttaataatgt ggatttgcca ctaccagatg tacctacaat agaaataaat
     8821 tcgccttttt caatagatag agttacacca tcaagagctt ttgtaatgtt tgaactttgt
     8881 ccataatatt tctttaaatt aattacttgt aaaatactca taataatacc tccttaacat
     8941 cttgtaaata cagtataaaa tcaaaatgtt gcagaatcat tacaaataaa atatttaaaa
     9001 atttatttat taggtaagaa gatagaaaaa gtcgaccctt ttccaatatc tgatgtaact
     9061 ttaatatagc caccttgaag agatataatt tttcttgaaa gatatagacc tattccaata
     9121 ccttctatgt catgtacatc ttcttcacga taaaatcttt taaagatttc tgcttgatga
     9181 ctttctgata ttccttttcc attatcaata atatctattt ttgtatacat aacccaacat
     9241 tctgtagcta cacgtatctt accattagaa tttgtgtatt ttacagcatt atctaatata
     9301 ttaaaaatag cttcagaagt ccatttcttg tcatgaatta tatataaatt tgggtcacaa
     9361 tcaactgtaa cttcaatatt ctttttttca gcatcaaaca aaattccact aagtgctgta
     9421 gcaattgttt cataaattga atttttcttt ttagatagtg tgattatacc tgtctctagt
     9481 ctggaggttt ttaccataga ttgcattaaa aaatctaatt tatctaattg gttttccata
     9541 tccattaaaa aatccttttg aatttctgtt ttaaatgttt gatttagtag agtagtatta
     9601 atcattttta aatttgcaat aggagtttta atttgatgag atagatcaga aactagttct
     9661 tgtaaatcag ctttctctat agcaatacta ttgcgatttt cttgaataac ttcatacatt
     9721 ctaattaatc tgtgattgat acgtgataaa atagtttctt cttctaattc aatttcaata
     9781 ttgtcttcac tattcatcat actatctaaa gtgtgacata ttttagaaca aaatataact
     9841 atttttttat gaagtaaaaa tattattaat gctataaatc cacaaagtac tattgtatat
     9901 attaataata gagcaattag ttttaaatca tcagttatta gataaattga aatattaaat
     9961 aaaatagctg taaaaattat accaacagca attattaaat aaatagaact aaaagaaaat
    10021 tttttaaaat tcatttcttt cacctaacca catatatccc ataccatata ccgttttgat
    10081 atacttaaga ttgtcttttt caattttgct tcta
//
LOCUS       DAEPLF010000126        10076 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEPLF010000126 DAEPLF010000000
VERSION     DAEPLF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.126"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            319..1749
                     /locus_tag="KR314_002516"
     CDS             319..1749
                     /locus_tag="KR314_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0328891.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1852..2955
                     /locus_tag="KR314_002517"
     CDS             1852..2955
                     /locus_tag="KR314_002517"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBH0328892.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMTYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            2970..3206
                     /locus_tag="KR314_002518"
     CDS             2970..3206
                     /locus_tag="KR314_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0328893.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEKACQEA"
     gene            3223..4272
                     /gene="vorB"
                     /locus_tag="KR314_002519"
     CDS             3223..4272
                     /gene="vorB"
                     /locus_tag="KR314_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBH0328894.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4274..5014
                     /locus_tag="KR314_002520"
     CDS             4274..5014
                     /locus_tag="KR314_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBH0328895.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            5017..5556
                     /locus_tag="KR314_002521"
     CDS             5017..5556
                     /locus_tag="KR314_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBH0328896.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLLEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5680..7779
                     /locus_tag="KR314_002522"
     CDS             5680..7779
                     /locus_tag="KR314_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBH0328897.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKMIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWALDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNTKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8046..8561)
                     /locus_tag="KR314_002523"
     CDS             complement(8046..8561)
                     /locus_tag="KR314_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBH0328898.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8653..9864)
                     /locus_tag="KR314_002524"
     CDS             complement(8653..9864)
                     /locus_tag="KR314_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBH0328899.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDFPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         3818 a         1236 c         1842 g         3180 t
ORIGIN      
        1 taaaattata aaaatttaaa atcactagaa atatgataaa ataatttaaa atacgcattt
       61 caatgacatt tcaagacaag aaaataataa aaaacaaatc taaattaagc ctaaatagct
      121 ttttagttaa ttttagtagt aaaaaataaa acttaaaaag tgtttgacaa ttatgaattt
      181 taagaatata attaaattaa aattatacac atataaaaat atactcctga atcaagaatg
      241 agatatttaa gattcaaaat atatattatt atacgtgaat aatgaaaagt aagaaaattt
      301 tataagggag gggtagatgt gagtagtcaa gaaagcaaac caaaaggaaa tcttatatca
      361 aattttagag atgatatgag agaccctaag aaaagagcaa tatacacata ttcaagttct
      421 ataaacttag cgtacaattt tataggttac tttgtatcaa ctgttacaaa tattttttta
      481 actgatgtgg caaaacttgg tgtaattatg gcaggtggta tgcaaattat acaatctttc
      541 ataaaaattg ctgtagctcc tataacaggt acagtatttg acaaacaacc attcaaaaaa
      601 ggaaagtatt atccatggct tagatatgca ccagcagcgt tagcattaac atatggaata
      661 tttttcatag ttccattaat gaaaataagt ccagcattac ttttaccttt gatgacagct
      721 ttattattaa tagcatcttg tgctcagcaa gtagttttca ctataattta tgctatttac
      781 cctgtagttg ctaagacacc tagagaccga gttataggct caactactac taatatattt
      841 aaagaagttg gtaaattctt agtaggtttt tcatatccgt tattattagt tttatttgca
      901 gatgtattag gtggagaagg aatgggttat ttaggtacct acttattctt tgcagcagga
      961 tgccttttag tattttggtt ttcttcaaca gagataataa aatctggagc agaagaagaa
     1021 gttcttcaaa aagaagagaa gccaaagatt aaagcaagtg aaatggcaaa agctctattc
     1081 actaatgtac cacttatgat ggcattttgt ttagaatttc ttatatgtat aagatctata
     1141 ggattaggac ctttggctcc atattatttt aaatatgtaa tagaagatga acgtggatta
     1201 gcagtatttt tatcaatcat gcctttggta agtgtagcat tcatgttttt cgcaccagtg
     1261 tttataaaaa tctgtagaga gcaaaaacta gcttcaataa tttctttttc tatatgtgca
     1321 ctatgccact tattggttgc atggactcct tgggggaaaa ctactttagg agtaactatg
     1381 ttgctggcaa ttggtggagg tttttcaaat gtagtatcaa taataaatct taatttcttt
     1441 gccggttctt gtgattatgg gcattggaaa tcaggaaaag acttacctgg cttaagtatg
     1501 tcactatatc cagttgcaat acaagtagga gtattacttg caactactat aagaacagtt
     1561 ttaatgaact caatgggata tcaagctgat atggtagtca cagaagcagt taaatcaggt
     1621 tttataaata tgatttctta ttctatggca atacctttaa taatagctgt tgttatagca
     1681 atactatatc cagtaagtga caagaaatta aaccaaataa gagaagagtt aaatcaaaga
     1741 aatgcttagt taaaagaaag ttaagtagat ggttaattta agctattaaa gtaagtagac
     1801 gcaatagcgt ttatataaat caaaatacat atacagaggg ggcttttaaa aatgatagaa
     1861 aaattattga gaaataccgt aaaagaatta catcaatatg ttccaggtga accaatagaa
     1921 aaagttaagg aaaaatatgg tgtaaaagaa ataataaaat tagcttcaaa tgaaaatcct
     1981 ctaggaccat caccaaaagc aatagaagct atgattgaaa tgctaaaaca aggtcaactt
     2041 tatccagagc cagaagctaa cgaacttaga agaaaattag ctgaaaagct agatttaaaa
     2101 ccagaaaatt ttattgttgc taatggtgcg gataacgtta tcactctaat aggtgaggct
     2161 tttataaata gaggagatga agttatatat tgtaacccaa catttccatc atatagaact
     2221 gcaactataa aaaatgaagg gataccagta gaagtgcctt taacagaaga ttataagtat
     2281 gatttacaag gtatattaga taagattaca gataaaacaa aattaatatg tgtatgtaac
     2341 cctaataatc caacaggaac aattgtggat gataaagagt tagaagaatt tttaaagaaa
     2401 gtccctgaaa atattataac tatattggat gaagcatata tagaattttt aacagttcct
     2461 aattatgtgg atggacttaa gtatgtaagg gaaaattata acgttatagt aacaagaact
     2521 ttctctaaaa tatatggtct tgcagggctt agagtaggat atggtattgc taaagatgag
     2581 ataataagaa ctttatttac agtaaaagag cctttctctg caaatcgtgt tgctataagt
     2641 ggagcaacag cagcacttga tgatgaagag tttataaaag aaacatatga attaaataga
     2701 gttggaatga cttactttaa agaagagttt acaaaaatgg ggtttgatgt tgtagattca
     2761 caatcaaatt tcctttatgt agatatgaaa acagatatac ctaagttatt tgaagacttg
     2821 aaaaaaagag ggtttgtaat aagaccaagt gctacacatg cacgtgtaag tattggaact
     2881 atggaagaaa ataagaaatt tgtagcagtt ttaaaggaaa tattaaatat taaaggagag
     2941 ccaagtattg gataatagat agaaatatta tgaaacgggg tgtagatatg aacttaaaaa
     3001 taaacgcaga gagatgtaaa agttgtgaat actgcgttat atcttgtaaa aaaggagcat
     3061 taaaaatatc aagtaagata aataaagaag gttatgctca tgtagaagtt gatgagagta
     3121 aatgcatact ttgtggaatt tgctatcagg tgtgtccaga taatgttttt gaaataatta
     3181 aagaaaaagc atgtcaagag gcttagaagg agttgggata atatgaaaaa gatgtggaag
     3241 gggaaccacg ccattgcaga agcagcccta agaggaggtt gtgaatttta tgcaggatat
     3301 ccaattacac cacaaacaga ggtaatggaa tttttatcac atagaatgtc tgaacttgga
     3361 agaactttta tacagtccga aaatgagatg gctgctatat atatggtata tggagcatat
     3421 gcaagtggaa tgaggtctat gacgagttct tcaggaccag gaatatcttt aaaacaagaa
     3481 gggatttctt acttatgtgc aaatcattac ccttgtgtaa tcgtaaatgt acagagatgg
     3541 gggcctggtc ttggctcatt ggactcagca cagacagact atctaagaga tactagaggg
     3601 ggaggaaatg gagattatca tttgatagtt tatgcaccaa acagtattca agagactgta
     3661 gatttgatgt ataactcatt tgatgtagcg gaaaaataca gagtgcctgt cgaaattttg
     3721 acagaagcag ctttaggtca aatgatggaa ccagtagaat ttccagaatt taagaagaga
     3781 gaaggagatt taggttggac ttatgatgga agtaatagag accatgcaaa ggttgcagat
     3841 aaccaaaagc caactttttg tatggagaaa cgtatgagaa taactgaaaa tgaacaacaa
     3901 tgggaagatt atcaagtaga agatgcagaa tatgtgtttg tagcgttcgg tataccatca
     3961 agaacaacta tgaatgctgt tagaagactt agagaacaag gtgaaaaagt tggaataata
     4021 agaccaataa cagtatggcc tttcccatat aaagcatttg aaaaggtaag taaagatgta
     4081 aaaggattta tctcagtaga atcaacagac acaggtcaat tagtagaaga tgttgcttta
     4141 gcctcaaaga aagtatgtaa agaaaatgta cctgtgtatg gtttatttag tggaaatcat
     4201 ataccaaaaa cattgcaagt aatggacact tatagcaaaa taaaatctgg cgaaatcaag
     4261 gaggtgttct aatatggcta cagaaaaatt aacacctagc gttgtttcaa taccaaacaa
     4321 gttttgccca ggttgtggtc atggaatagt aaatcgtata atagcagagg taatagaaga
     4381 acatggatat gaaaaaaatc atgtattaac attaggagtt ggttgtgtct gcaatatgaa
     4441 ttttagctgg aatggtgata agatgcaaac tgcacatggt agagcttcat ctacagctat
     4501 aggagttaaa gtagcacttc cagatacatt agtaatgact taccaaggtg atggagatgc
     4561 ttatgttata ggtctttctg aaactttaaa tacagcatat agaaatcata atgtgactgt
     4621 atttgtaata aacaataata attttgcaat gactggtgga caaatgtcat ggaccactat
     4681 gccgggtcaa gtcacaacaa caagtgtaaa tggaagagat ataaagacta ctggtagtcc
     4741 actgaaagtt cctgagatgg tggcaaactt ctttgatgta gcatatgtag caagaggttc
     4801 agttcatagc ccaaaggaaa taattaatct aaaaaaatat ataaaaaatg caatagaagc
     4861 tcaattaaat ggagagggat attccttagt tgaaatatta gcaccttgcc caactaactg
     4921 gggtgtatca ctagagaaat ctatcaaatg gatggaagaa gaaatagtgc catattatgc
     4981 tcttggcgaa tttaagcaaa gggatggtga atagttatgg aaaaagagct agtatttgca
     5041 gggtttggtg gtcaaggagt tttaactctt ggtctgattg tagctgagtc agcacttgaa
     5101 ttggggaaac aagtaacttg gatgcctgca tatggtccaa ctatgagagg tggaaaagcc
     5161 tatagtgtag taaagttttc tgatgagagt ataggtgggc ctgatatgga agaaatagat
     5221 gttttagtag caatgaataa accatcttta gattatataa atttagttaa agaaggtgga
     5281 acagtagtta ttaatacatc tgcaatagat gagaatgtaa cattaagaga agatataagt
     5341 gtagttaaaa taaattgtca ggaattagct caaaaagtaa acaatccaaa agcagcaaat
     5401 atagtagtac ttggagcatt aattgctaaa actgggttac ttgaaaaaga attagcttta
     5461 aaaactatgt gtgatttctt tgaggaaaaa ggaaaaggaa agtttaatgt tcaaaatgaa
     5521 gcagcattca tggaaggcta taataacgcc atttgataaa ttacccttat cttaagtgat
     5581 ttatctcaag atttaataat ctttagtgcc aagagaaact tcatcttgag ataaaattca
     5641 cttaaaatca caactataaa catataggag cgtgaattta tgagtttgga aaaacttatt
     5701 agaccaaaaa gtattgctat agtaggagtg actgataaac ttggttttgg aagaagtgca
     5761 gcacttagta tagtaaaaag taaggaaaca gatagagtat attatgttaa tcccaaaaga
     5821 gaagaattgt ttgggagaaa atgttataag atgatacaag aagttccaga agtagtagac
     5881 tgtgtagtag tatgtacgcc tagaaatgta gttccatctg tattaaaaga ttctggagag
     5941 ttaggaacaa aggcagcagt ggtatatgct agtggatttg cagaggaagg tacagaggaa
     6001 ggtacagatt tagaaaatca acttatagaa atatcaaata catacgatat gaaaatattg
     6061 ggtccaaact gcatggggtt gctaaattgt atagataagg ttaatctatg ggcaggaggt
     6121 tctaaatggg ctttagatac taaaaaacct ggtataggaa ttgtaggtca aagtggattt
     6181 ataactgctg aaattgtttc aagtgattat tttaatatat cttatggttt ttcaacagga
     6241 aatggaaata tagttacact agaagatgca gtagatttcc ttgtagatga tgattatgca
     6301 tcagtaatag caatttacct tgaaggttta aaaaatcctc aaaaatttat agatgcttta
     6361 aaaagggcag cacaaaaaag aaagccagta ataatattga aatcaggaag gtcagaaaaa
     6421 ggtgctattt ctgctgcctc tcatacagga aatttagctg gttcgagtaa agcttttgaa
     6481 agtatttttg aaaaatatgg agtaatttct gtggaaaatt tagaacagtt tatgtgttta
     6541 gcacaagctt ttagtgtttt agatggtaat ttacctgcaa attctaattt tgcagcaata
     6601 aacttctcag gaggagaaaa tactatttgt gcagatttag cagaagaaaa tggtgtagaa
     6661 cttgcagaaa tatcttctga aactaaagaa gaaatgaaaa aatatcttcc aggatttgca
     6721 acacctaaaa atccactaga tgctacaaca gcattattcc atgaaaagga tatgatagta
     6781 ggtttattac atacctttga aaaggatgat agtgttggat ttactatggt aggtgcaaat
     6841 ataagagatg aagaaaatga aatgcatgaa accctttgta aagctgtaag tgaagctaga
     6901 gaacaaggat tgaaaaaacc tgtgtttgca gttcctacac ttgaaggaaa tagatattta
     6961 gattatagga ctagattaga agataatcaa gtgcctataa tgtcatcagt tggaactaca
     7021 tttacttgtt ttaataaaat ggctaagttt atagattatg attattcaaa aagaacttta
     7081 gaatttaaag ctgttaagaa aagagagtca aataatgtag tagcattatc agaattagat
     7141 agtaagattg agatgaaaaa atatggagta ccagttccag gacaaggtaa tacaaaaagt
     7201 attgatgaat tagatgaaat cttaaaatat attaagtatc ctgttgtttt aaaaataaat
     7261 tcttctgaaa tattgcacaa atctgatgtt ggaggagtta aaataggaat taagaataga
     7321 gatgaggcag tagatgcata taatgagatt ttaactaatg taaaaaaagc aaaaccagat
     7381 gctaatattg atggtatatt agttcaagaa atggtagaaa gtggtataga aattattatt
     7441 ggtataacta atgatgacca atttggacca atgttattag tcggtttagg aggagtattt
     7501 gtagaggtat ttaaagatac tacattatat ccattaccta taaatcatga tgaagctatt
     7561 atgatgctta aaaaactaaa atcatttaag cttttaaatg gctatagagg aagtgagcct
     7621 tgtgatattg atgctcttgc agatatgatg gtcaaattag gaaaatatgc ttatgaaaat
     7681 aaggatgaag tcaaggaaat agacttaaat ccagtgtttg tatatcctaa aggaaaaggc
     7741 gtatgtgcag tagatgcctt aatagttaaa tataaatagt ttataagttt tggtattcaa
     7801 ccataattaa agcaccgact gaaaatcaca ttttgtaagt tagatagtgt gattttcagt
     7861 caccccaaat ttaatttaaa aatattttaa aatgaaagta tagttttgag tttaattgtg
     7921 aaatagcact gtataaacaa taaaatacta cgtataaaac gaataataaa aggtgtttta
     7981 atgataagaa tcaatcttga ttttcattaa aacaccttct ttattatatt aaaaattttt
     8041 aaacgttata ttgtcacagg tgggtttgtt ggagctgcat atgtacgatt agcaaattct
     8101 tggtcaacaa agaatagacc accctctcca gcaagtttaa cacgttttat attgtcttca
     8161 caatttgttt cttcttctgc ttgttcagat ataaaccaaa gtaagaattg agaagttcta
     8221 aagtctgatt cagagtttgc tacagctgct aagttgttta ttaaagctgt aacctttttt
     8281 tcatgagcta acgtacgctc aagaatatcc attgcagaag taaagtctga atctggcatt
     8341 gggatagcat caagctgaac ctttcctcct acattatgta agtatttgta aaaaaacata
     8401 gcgtggtctg tctcttcttt atattgaaca taaaaccaat tagaaaatcc tttaagtcct
     8461 tctgcttcaa ggtaagaaga catagaaaga taaagatacg cagaatataa ttcatggtta
     8521 atctgctcat taagtaattt ttccatttta gctgaaatca tttttatacc tccaaatttt
     8581 aaatgctctt ataaaatata ataatattaa atggaaaaat attcaataaa agtgtttaaa
     8641 tattttacat atttaataaa aatttatact accattaaaa aaattataac ttacaatggc
     8701 tatagagcca attaatggtg catttgctcc aaaatttgaa tgccttacac ttattttttt
     8761 atatttagaa aaacttgctg aagcagtcaa tttttttaat aatgtatttt ctataaatgt
     8821 accaggtata ttaaagtcat agcctattat tatagaacta caatcaatca atgtaagtgt
     8881 attaactaat gcatatgata cgtatgtaca aaattcatca agcaaaaaca tggcaagact
     8941 atctttatta tttgcctcat ctattaactt gactaaagaa aaatctttac agttaattaa
     9001 tggggagtta ggataaatat ggctcaatga ttcaattctt ttaattagat tactgacact
     9061 tgtataataa tctaaacatc cattgttacc acaactgcac aatggtccac taaaattaat
     9121 tgatgtatgt cctatttctc cactttgtcc taggttgcca gtatgaagtt tattttctag
     9181 aactagacca gctccaatac cattcattat gtgtaggtat atataatttg atatattttt
     9241 acctaaacca tacatttttt ctgctaatgc acccgaatta gcatcattta tcaaaaatat
     9301 tggaaaatca gacatttctt ttataaaatc tactatattt aggtttgata catttccaaa
     9361 gtctggggga tttagtatgg taccatttat atcatctact ggacctattg aagaaatacc
     9421 aattccaatt atatctctct tgttgatttt cattatttta gtaaacattc tttttattat
     9481 atcttttaaa acatcatttg atagacattt agggtaagtt tcagaaagtt gttttacaat
     9541 attacctttt aaatctgaaa taattacctt acatatattt cttcttatta ggattccaca
     9601 aatacaaggc gatatattag aaatatctaa taaaataggt ttacgacctg atgaatgtgc
     9661 atttaaagtt gatgactcta tttccctaat catattttca ttaataagtt cagtaactag
     9721 atttgataaa gtcattttag taagtcctgt agacttagct aaatcaactc tggacatagg
     9781 tgtatttgta gaaagtaatt tcaatattat taacttattt ttaattttta catctttgat
     9841 gttagaacct tgtttgttac tcatcataaa tctccttaaa taaagttaag tgttttattt
     9901 attttcaagt atactatcac cttataatag agtaacattt ttttacttga aaatatcatt
     9961 aattagaata tatcacatta tatatcataa aacatataaa aaaatgttag aattaaatta
    10021 aaagtgaaaa gtagattgat atataaatag taaatactta attaaaaaaa gataat
//
LOCUS       DAEPLF010000127        10064 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEPLF010000127 DAEPLF010000000
VERSION     DAEPLF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.127"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(54..932)
                     /locus_tag="KR314_002525"
     CDS             complement(54..932)
                     /locus_tag="KR314_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0328900.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(949..1266)
                     /gene="trxA"
                     /locus_tag="KR314_002526"
     CDS             complement(949..1266)
                     /gene="trxA"
                     /locus_tag="KR314_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBH0328901.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(1416..2648)
                     /locus_tag="KR314_002527"
     CDS             complement(1416..2648)
                     /locus_tag="KR314_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0328902.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            complement(2638..3309)
                     /locus_tag="KR314_002528"
     CDS             complement(2638..3309)
                     /locus_tag="KR314_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0328903.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(3325..4161)
                     /locus_tag="KR314_002529"
     CDS             complement(3325..4161)
                     /locus_tag="KR314_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328904.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(4187..4861)
                     /locus_tag="KR314_002530"
     CDS             complement(4187..4861)
                     /locus_tag="KR314_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH0328905.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKRKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(4875..5603)
                     /locus_tag="KR314_002531"
     CDS             complement(4875..5603)
                     /locus_tag="KR314_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBH0328906.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIKDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSGLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(5730..6419)
                     /locus_tag="KR314_002532"
     CDS             complement(5730..6419)
                     /locus_tag="KR314_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0328907.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLRVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMIGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(6427..6990)
                     /locus_tag="KR314_002533"
     CDS             complement(6427..6990)
                     /locus_tag="KR314_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0328908.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(6992..7960)
                     /locus_tag="KR314_002534"
     CDS             complement(6992..7960)
                     /locus_tag="KR314_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBH0328909.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(8061..8104)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8469..9098
                     /locus_tag="KR314_002535"
     CDS             8469..9098
                     /locus_tag="KR314_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0328910.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKAIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQSY"
     gene            9073..9477
                     /locus_tag="KR314_002536"
     CDS             9073..9477
                     /locus_tag="KR314_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBH0328911.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            9480..9953
                     /locus_tag="KR314_002537"
     CDS             9480..9953
                     /locus_tag="KR314_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH0328912.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
BASE COUNT         3361 a         1672 c         1006 g         4025 t
ORIGIN      
        1 tatttcaaga cagtttttta taaatatctt taaaaagtta atcaatataa atattaagcc
       61 ctctttaatt tatctaaata gtaaacagca tttaaagctg ctatttgccc ttgaccagct
      121 gcttttatat aagaataggg ttttccaaca caatctccag cagcaaagca tccatctata
      181 ctagtcttca tattattgtc aactttaata tgaatgccat caatctctat tccaggaacc
      241 aatgcacttg gagccgtact atcttttata acgaatactc catcaacttc aatgtggttt
      301 tctttaaaag atactttgtt tacaagttta tctccatcta tctgtactgg tctgtcatgt
      361 ataacttcaa tagaatcatc taggttatct gaacttctca taagattatc ttttttgtac
      421 ataggtataa aataagtctt tgaagtgagt tcacttaaaa aatttgcttc ttctttagat
      481 tcttcattat atcctataac tgctaccttt ttatttctat aaagtggtgc atcacatgta
      541 gcacagtagc ctacacctct tcctagaaat tcttcttcac ccttgattgg tctagtatac
      601 tctaccccag ttgcaaggat tacagtagta gcttcataca tatcatttcc agacattatt
      661 gtaaagtatt ctcccatagc ataaatatta tttattcttt tattttcaat tgaaatatcc
      721 atactatcta tatgtgattt aaacttgtct tttaattcat caccacttat atcataaaaa
      781 cctaaataat tatctattga aggagcttta acaagcttat tacttaaatt atcattacca
      841 aacatgataa ttgttttatt tcttattttt gcatttatag cagcagacaa tccagctgga
      901 ccactgccga ttatagctat atcatatctc ataatactca cccatttttt ataaatatga
      961 ttttacttgc atttcaattt tgttctttgg catgaatcca attaaagttt ctactggttt
     1021 tccatcttta aatataatca tagttggtac tgtagataca ttgaattgtt gagctatttc
     1081 taagctctca tctatatcaa cttttacaaa tctagcttta tccttcatct cttcacctaa
     1141 ttctgcaaat acaggtccca acatgttaca aggtccacac caagttgcaa agaaatcaac
     1201 tacaactact cccttactac cttcaacact acttctaaat tcagatgtat ttataacttt
     1261 tgccattttt aaatcctcct attaaacttt attatcccta tataattata ctatgaaatt
     1321 tataaatata aatttacttt tataaggtct ttttatattt tatactaatt agtataccca
     1381 ttcttcattt ctaaaaacaa ttttttatat ttttattatt ttaacttttt tggtattgac
     1441 aattcaaatg tagttccaat atctacatca gaagttacat ttatatcaat agataagttt
     1501 aaactaatca tgcgagctat tgaaagtcct attccagaac cttctaaatc tttatttctt
     1561 acattttcac ttctaaaaaa tctatcaaat ataagaggta tctcttcttt ttttatgcct
     1621 ataccagtgt cacttataag taaagttacc tcatcctcta tatcttcttt taattctaat
     1681 gctatctcat cacccaattt tgtatattta aaggcattat caatgaaaat taatataagc
     1741 tgtcttaatt tatttttatc tgttattata actttattct tcagtttaga attaaacacg
     1801 aatctttttt cctgaaactc agcaatgtca atatagtctt ctgatatttc ttccaataat
     1861 ttttctaagt ctatttcttc taaattaact ttaacaatag agtcctcttt agtaagagac
     1921 aataaatcag ttatcatttt tttaagtctt ctagtttcct tcattgcagt agctattgtt
     1981 tcaacttcat catttactgt actttctggg gattttaaca tactttctaa ttttgatgaa
     2041 actattgtta ttggtgttct taattcatga gaagcatctt gtataaactt agcttgattt
     2101 ttccatgctg tttctattgg aattaaagct tttctagtga gatatacagc cacaaagtaa
     2161 gtaattatta cagaaattaa tattccaatt ataagaacag atgtaagctg tcttaaagaa
     2221 ttcatctcag aatctatatt tctaattatc tgtatttggt atctacctac atctacattt
     2281 aattctctaa aagtatatcc attttctgtg tatttaaaaa acgaattttt tttatctagt
     2341 cttctatctg gaagtaactc atcaaaatat tcattttgag tatagtaact tatccttcca
     2401 tcttcatata catacaccat atctttagga tctttcagtc ttattgggtt caaaaatgaa
     2461 gttcttttaa attgagagac tatatattca agttcatcat ttatctcact atcaattcca
     2521 ctatatgtaa gtcccctaaa atatgagtat ataaatatag aaaagattat taaaaaacta
     2581 cctactacaa taatatacat ttttattaaa tgatttttag tactggtaaa tacattctta
     2641 tttatcatcg aatatatatc ctaccttacg tttagtttta atatatttat catatccata
     2701 tttacttagc ttttttctta gattacttac atatacttcc actatttcaa tagttgcatc
     2761 tgaatcaatt ccccatattc tatcataaat ctgctctttt aataaaattg aacctttatt
     2821 taatacaaaa tactcaagca agttaaactg tttgttttga agttttattt cttctttttc
     2881 aatatacact cttttttcta gtgtatctaa atataaagat ttaaattcta atccgtttct
     2941 ttcttttatc tttccattag tccttaatat agcgtatacc ctagctacta actcttccat
     3001 ataaaaaggt tttgtcaaat aatcattagc tcctatagta aatgcttcta ctttgtcatc
     3061 taatgtttct tttgctgtca atataagtac aggtgtatct atattattaa ctctcatttt
     3121 ctttaggata tcaaatccat ttatattagg aagcattaag tctaaaataa ctaaatcata
     3181 tatattttga tttactaagt ataaagcttc ttctccatct tcacactttt cagtttcaaa
     3241 atgcttgcta agttcttcta ctacactttc taaaagtatc ttattatctt caactattaa
     3301 aaccttcata agtatcccct acctttattt gtctatctta actttatgag attttaagtc
     3361 aatatccaca ttggcttttt cttttatatc atcatacgta tatcccacta tttcaacact
     3421 ttttgccttt gttgaacaca atatatctat atctgtctct tctgaaggct ttaaaatctt
     3481 atatgatggt acacgattta ctgttaaata ttctccattt tcattaactt ctgctgtctt
     3541 aagaataata tttcctaaat cttttgatga tgaatttttt acttttactt gatacgtaat
     3601 tccctcattt gattcattta cttttttaag ttctgatgta gatagcacct catactgact
     3661 actattttct agcttaatat tattatttct tatattaata gtattttctt ttaaattaac
     3721 atacacaatc ttaccctctg cctcatattc atatgcataa acttctacac cctttacaaa
     3781 agttttatgc ccacattcta tgtaagcaac tttcccaggc attaaagtca tatccaacaa
     3841 agccttacta tccgaagata taattttttt attctcatca tattcattat atatcaattc
     3901 aacattggat atattaaatg ttgaattatt ttctaccttt gttgatgtat ctactttatc
     3961 accttcatct atatttataa tttctgattt tcctatattc aatacttttg catttccctc
     4021 atctattttt atctcatttt tatcttctgt ttgaatatca actgaatcag gctgttcatc
     4081 tttttcacat gctgtcaaca taaaaataaa tattgttaat ataatcatta gagaaattga
     4141 tttttttact attttattca tattaagcac ctcatatagt agtattttat agtagatttt
     4201 tatacttttt cataaattct ttcaatgcct gtgtaagtat ttgatgtttt tcaaaattcg
     4261 aattactaca taatctttca aaatcattcc atatttcttt atctatacgt attcttgttg
     4321 tttttaattc atctatgtgt tcatcatcta tatgtacatc ttttttatta gatacttttt
     4381 taggctttat attgtaatct tttacttcta aataccaatc atatacttca caaagcttat
     4441 ctaaatcttc ttgagttata tttatttttc tatctttctt aggcttaaca ttttgttttc
     4501 gctttgcatt ttttaaacct gtatcattaa actcaatttg ttcaacactt ttacttaaag
     4561 ccttttcatt agaaatcttt tttttggtgc ttactttttt tctattactt atagtattct
     4621 ttttactttt ttcatactcc ttagctttct cttcttccaa gtttctatta tccaaacttt
     4681 tattataatt taattcgtct tttaatatgt atttaccttt ttctgactta tatccttttt
     4741 tatttaaaaa agttcttaaa gtactttgac taatctttag attttgagct atctctgcaa
     4801 atttaaatcc tttgctttga agttctaaaa atttgtcaat tctgtcttca ttgttattca
     4861 ttaatattcc tccgtcaaca tcttcttgtc tttcttttca tacttcttat tatattcttc
     4921 ttttctaaat ctactctatt tgattcaatt attttttgta gcattttatt ataagtgaaa
     4981 tcatctaaat tattattttt tatatagaga aaagtttcct cttcacattt aataaaacaa
     5041 attgatagca accatgccat acccatttta acataatatt cttcactttg tatattatta
     5101 cagtattcaa atatttcttt aatatgttta tcatcaacat aatatattaa aaacataact
     5161 aatgcaaatc taatttccca tggattcttt gaataaacat attttttaag tatatcatac
     5221 atattttctt tatatttttt catagacttt aaaccagaac aaaacccatc gcaaactgac
     5281 caattatcga ttttaggtat aaatcttttt acatagtata aagtttcttc aaaattacta
     5341 tttatattgc caattaccat accttgtagc ctaacttctt catagtatgt atcctctgct
     5401 acttctaaaa attccttcca attcccttgg cttatttctt ttgataactt tcttaaatta
     5461 ggaactctaa tacctattat attttctgtt ccaggtataa gctttttatt aaaatctcta
     5521 tattttatat cttttataga catcatatat tctaaaagtt cattataatc caatttattc
     5581 cattttcccc tatcaaacat catttaattg ctccttaatt ccttttttct tattgtaaca
     5641 taaaactcta caaaaaaagc ttctaactat tagaagcttt ttcaaaaaca taagaaactt
     5701 ttcaatttta aatttaaact aaattaatgc taatctccta caccaaagtc tgtattattg
     5761 tataaaactt ctatatttgg attttcatcc aagaatttat acttactaac aaaccatttc
     5821 actaactctt catctctttt tatactagaa gaattattta taaaaacatt cactgtcgct
     5881 ttttcaaaag tatttaggat aatctctata tctttatcta tcatctcttt tgtttgtcct
     5941 tttattccaa tcataataca aggtgaatca aaatattctt ttacatcttg tggagtctta
     6001 aaatttgcat ttttatttaa aatattattt ctaaaatcat tgtcaaaggt ttcaactcca
     6061 attttaaata ccacaggaat ttcaaaatac tctctcattt ccttaagcct atttttgtaa
     6121 gaccaatgac tttctaaaaa taattttttt atattcttct cttttataat acactttatt
     6181 ttttctaaag tatccttagg taattcaaaa cagcttccag aatttatgac ttcaagaact
     6241 ccatatttcc cagttacatt ttttaaaacc cttagattta atttattcat ctcttcttct
     6301 aaattagaat tatcatctat ataatcacaa aaagaacact ttccccatat acaaggaaat
     6361 cctttaagca gaacaatttc tctttgattt ttatttgtta tctcactata tctatccatt
     6421 ttctatctat tttcctccag tcaatgatac ctttaaagtt tttggaactg catttaatcc
     6481 taaattcact aaaacaactg ctaacagctt atctccataa tcagtaaata cttgggttac
     6541 aaacacacta aaaacatctc ccagtcctaa cactttcaag atttgcacta tataggatga
     6601 acctgaagag gtcatacctc caaataaaaa tgctgaaatt attgaactta ttatagatgt
     6661 tggaatagca aataaaagta ctcctagtgg agtttttaaa ccccttaaaa aattcttttt
     6721 aaatgctatt ccagcaagta atccggttga tatttgaact ggtgcaaaat agagagaata
     6781 cacatcaaac gtaattccac ttattaaact tccaaacaca cctgtaacaa cagcgtattt
     6841 aggtcctaaa agtgaagcta tcattatagt tcctattgaa tctaagtata caggtaatct
     6901 taatcccata gctataaaag cgcctattat atttatagaa ataccaaaag ctataagtgt
     6961 aatagttttt acacatattt ttcttttcac actatatcac tccttccaca gagtctataa
     7021 tgtcttcata acctttaaaa agcctcttta aaaacatgta cataaatttc ttctcatcaa
     7081 cttcatttag aacaattgca ttaggatttt ttttatagaa attgaaagaa tccactatag
     7141 actgacccat agctattcca tcttctacaa cttctacata tgattcaaat cctttacata
     7201 tacttctgtc tataaagtac gctactgcta gagggtcatt tatcacacag ccaattattc
     7261 cttcctgttc ccaatggaaa tcaatataaa atctagttat ttcagttata tactttgcca
     7321 tcttcttatc aagtctattt ataaactcaa taatattagg agtaagtaca atttttctag
     7381 ttacatctaa acctaccatg tggatttttt tagataaatt cttgtaaaca taatctgctc
     7441 catgtgggtc tacccaataa ttaaactctg ctactggaga gcaatttcca tgaatcctaa
     7501 atgctcctcc catagataca aactcatcga gattttcaaa tgctttctta tctttaataa
     7561 gtgctttagc tatatttgta agtggtgcaa gtgctattat tgatactttt tcatgattat
     7621 gtaaagtatt tattataaaa tccactgctc cattttttgc tttagctcct acaacctttt
     7681 gataaaagtt ttctccaata ccatcttctc catgtgtatc ttgagctgtt acaagttttc
     7741 tttttagtgg tgcttcttct cctatatata ctggaatatt tagtgaagaa cacatttgaa
     7801 gtgtttttag tgcattttct gcacctatat ttgctggaac atttccacaa catgtggtaa
     7861 ttccaattac ttctagctct ggtgagttta aagccagaag aattgccaaa gaatcatcaa
     7921 ttcctgggtc acaatcaatt attacttttc tcttttccat tttttatcct ccttgatttg
     7981 tggtcgagga gaactacaca tcttttttcc actttcaaaa aggtgtgtct aagggtatta
     8041 attatatact gccactatat aattccttag ttttttatac ttggagttct cgaacaagta
     8101 cagatgtaaa atcatatttt taggctactt actatagact cttaaaaact atagactgtt
     8161 aaaaaatttt taaacatctg atttaatagc tataaaataa attttatagc ataaaagaat
     8221 ttattatatt ttaatgtatc ttattaaagt ttatttagaa ttatgaacca actttaataa
     8281 gatgtggcgc atttaaaccg attattatta tacaataata tcttaaatat tacaattaat
     8341 aacattaatt tattatccat aaaaatatat taacttatat tatttaataa tttattgaca
     8401 gtattaaaat taaattatac aataacagtg taaattaact taacaccgtt aagcaaagag
     8461 gtgattatat gagtatcaaa gaaagacgta aaaatgaaaa agaagaaatg aagaaaaaga
     8521 ttatggatgc tagcatcgaa attataaacc aacatggata tgaaaattta tcaataagaa
     8581 aaattgctac aaaaatagaa tattctccaa ctacaattta tctttactac aaggacaaag
     8641 cagagattat cagcgatatg accaataaac tttataacac agtagaaagt aatgctattg
     8701 acattatgaa taattgctca tcactaccaa tagataaaca aatcatagaa attatgacta
     8761 gctttataaa aactctttct agtgaaccag agatggcaaa agctatcatg catagtagaa
     8821 tgaatattat atttgcatct gaaaatacaa acaatactcc tagcaataat ggtataaaaa
     8881 tgcttgataa attcctttcc attggaattg aacaaggaat attcaaaaaa aatatagata
     8941 aaagctcatg gatgcttata agtgcattgt taggattcgt tttatgtgtt gttgaaaata
     9001 aactatattc tttacataat ttttctcaac ttatagaaaa tttcttagat attttagtgg
     9061 gaggacttta caatgaaaat tcgcaacaaa gctactaata aaaagaaaaa atatgtaaaa
     9121 atatctattg ctctattctt tgtatttata tgcattatta ttgtaattgt aagcaactta
     9181 aagccagaaa acttggtagt aaaagatata gatattaata atgttaaaaa tggcatttat
     9241 actggaagtg ctgataataa cttggtaaaa gctactgtgt ctgttgaagt taataatggt
     9301 aaaatccaaa atattaatat cttaaaacac gaccatcttt taggtaaacc tgctgaaaaa
     9361 attacaacaa gtattattaa acaacagtct ttagatgtgg atgctataac ttctgcaact
     9421 tatagcagta atactatacg aaaagctgta gaaaatgctt taagaaaggg agaataatta
     9481 tgaatactat tattatatat tcaagtaaat atggttgtac aaaagactgt gcaaatatac
     9541 taaaaaataa actatcagat aatgtcacta ttgttgatat taataataat aataaaattg
     9601 agttatcaaa atttgataaa attattattg gaagttctat atatgttggt tcagtttcaa
     9661 aaaaaataca agtgctatgt aatgataatg tagagttatt aaacaaaaaa caagttggca
     9721 ttttcctttg ctgtggcttt tctgaacaag cagataaata tttaaaatca aattttccat
     9781 catccttatt agaaagtgca aatgctattg gaatttttgg tagtgaagct cgattagaaa
     9841 aaatgaaatt tttagataaa cttattatga aggctgtttc aaaaggtaat tatgacagtt
     9901 ttagaatatc acaggataat atagataatt ttttaataaa tcttaatagc taaaataaaa
     9961 ctaattattt ctatgataaa ttcttttatt tataagtaat attgctcaaa atagtcttta
    10021 aactttatca aaattttatt tttcttagtg aaatctaatg aaac
//
LOCUS       DAEPLF010000128        10057 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEPLF010000128 DAEPLF010000000
VERSION     DAEPLF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10057
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.128"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            436..648
                     /locus_tag="KR314_002538"
     CDS             436..648
                     /locus_tag="KR314_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0328913.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            661..882
                     /locus_tag="KR314_002539"
     CDS             661..882
                     /locus_tag="KR314_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0328914.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            982..3138
                     /gene="feoB"
                     /locus_tag="KR314_002540"
     CDS             982..3138
                     /gene="feoB"
                     /locus_tag="KR314_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBH0328915.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTIIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHVPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            3164..3316
                     /locus_tag="KR314_002541"
     CDS             3164..3316
                     /locus_tag="KR314_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBH0328916.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            3496..4206
                     /locus_tag="KR314_002542"
     CDS             3496..4206
                     /locus_tag="KR314_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBH0328917.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            4291..5061
                     /locus_tag="KR314_002543"
     CDS             4291..5061
                     /locus_tag="KR314_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0328918.1"
                     /translation="MFEKIHKRFKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            5054..5785
                     /locus_tag="KR314_002544"
     CDS             5054..5785
                     /locus_tag="KR314_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328919.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            5819..6778
                     /locus_tag="KR314_002545"
     CDS             5819..6778
                     /locus_tag="KR314_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0328920.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(6876..7472)
                     /locus_tag="KR314_002546"
     CDS             complement(6876..7472)
                     /locus_tag="KR314_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBH0328921.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVNKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            7718..8317
                     /locus_tag="KR314_002547"
     CDS             7718..8317
                     /locus_tag="KR314_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328922.1"
                     /translation="MKLKRTLFVGLALVLAIGITACARDNEKPNENAGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKRMNDDLIAEGERLVKDIDERIEKLDKVSDEDMKKGQN
                     DFMKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            complement(8485..8952)
                     /locus_tag="KR314_002548"
     CDS             complement(8485..8952)
                     /locus_tag="KR314_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328923.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIEESYNFAKQKFKKRKNK"
     gene            9063..9974
                     /locus_tag="KR314_002549"
     CDS             9063..9974
                     /locus_tag="KR314_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBH0328924.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SVGFPLYSKVGAAGGYHVLKERILPPITFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENMRRKLVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSIRTIVPIGLYAMNGLWYCPAYCIKSESVKEFRADRIVKILSIENLSNKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKK"
BASE COUNT         3817 a         1090 c         1807 g         3343 t
ORIGIN      
        1 tattttttga gatagagata gtcagtagaa tcatagaatt aattatgttt ttatgattgt
       61 attagattaa tatttaaatt acattgaaaa gaaaaaaatc attactatag aataatatct
      121 ttatcaaaag aacattctta tgcagaatat ggttatgagt atctaagaga aatctcaaaa
      181 tatataggat agcagtatga atttttatag attttattaa tacaataaat caaatgagaa
      241 aaaaaatcaa aagaatacaa taatcacttg caaaaaatat gctattgtga tagacttaaa
      301 tcaaaattag gaattgtaag aataaggaaa aagataagta attattttaa cgagtcaaaa
      361 tttaagaaat tgtgatttat atataagata gaggttgttt gtaaatttaa attggttgaa
      421 gaggaggaat attatatgat gccattgata ttatcaaata caggtgaaga agtagttata
      481 aagaaaattt cgggaagtga taagacgagg acttttttaa atagtatagg ttttgtagta
      541 ggtacaaatg ttaaaatagt ttctaagata gatggtaact taataataga cgtaaaagat
      601 actagagttg cattagataa aaaaatggca agtagaataa taatataggg aggtaatgac
      661 atgaacagac tgaaggatat taagtgtgga gagactgtaa aagtcaaaaa gttagaagga
      721 gaaggagcta cacgaagacg tataatggat atggggatta caagaggtgt taatatattt
      781 ataagaaaag tagctcctct tggtgatcca attgaggtaa cagtcagaga ttatgaacta
      841 tcaattcgta agtcagatgc agaaaaaatt attgtggaat aataaagatt aattagagta
      901 gggttaatgt aattatataa tgagaatgat tttctgtatc acaaacagtg atgtatttaa
      961 ataataatgg agggaatgga aatgtcaata aaaataggtc tcataggaaa tcctaactgt
     1021 ggtaagacaa caatgttcaa tgggcttaca ggctcgtcgc aatatgttgg taactggcca
     1081 ggagtaacag ttgaaaaaaa aggtggaaaa ttaaaaggaa ataaagatgt ggaaatagta
     1141 gatttaccag gtatttattc tttatctcca tatacattag aagaagtagt tacacgtaat
     1201 tttatgctag atgataagcc ggatgcagta ataaatattg tagacgcatc aaatatagag
     1261 agaaatttat atcttacaac tcaagtttta gagctaggta taccgaccat tatagctctt
     1321 aatatgatgg atattgtaaa taagaatggt gataagataa atattaaaga actatctgaa
     1381 gttataggtt gcccagtggt agaagtaaca gcagttaaag gtcaaggaat tatggaggct
     1441 gctgaaaagg cagttgaact tgcaagtagt aacaataaat taaatttcaa attacctttt
     1501 gttgatgaaa gtaaggacgc aatagaaaaa attgaaaaaa taatagaaga aaagactcca
     1561 tatatagatg tagaaacacg ctggttagct ataaagctat ttgagagaga tgaaaatgtt
     1621 atacaaaaac ttgatatttc taaaacaata ttgaatagta ttgaagaaat aactagaaat
     1681 tgtgaagatg aattggatga tgatagtgaa agtataataa cagccaatcg ttatgaattt
     1741 attagttcaa taatttcaag tataataaag aagaatcgaa aaggtaagga aactgtatca
     1801 gataagatag ataagattgt aacaaatcgt atactagcat tacctatttt tgcacttata
     1861 atgtggggag tttactatat agcagtaagt tcattgggta ctatagcaac tgattggaca
     1921 aatgatgtac tgtttggaga aattattcaa ggcaatgtaa gtaattttct agcttcttta
     1981 aatgtagcag agtggttaca aggacttgta gttgatggtc taattggtgg agttggagca
     2041 gtactaggat ttgtaccaca aatcatgctt ttattcttat tattgtcaat attagaagat
     2101 tgtgggtata tgtcacgtgt agcttttata atggatagaa ttttccgtaa gtttggtctt
     2161 tcaggaaaat catttatacc aatgcttata agttcaggtt gtggagttcc aggagttatg
     2221 tcaactcgta ctatagagaa tgatagagat agaaaaatga ctattatgtt aactacattc
     2281 attccatgtg gagctaagat tccaataata gcattatttg caggggcatt atttggaggt
     2341 gcttcatggg tagctccatc aatgtatttc ttaggtattg caatgataat catttgtgga
     2401 attatattaa agaaaactag tttatttgca ggagaaccat ctccatttgt tatggaatta
     2461 cctcaatatc atgttccaag tgcaaaaggt gttttaatac atatgtggga tagaggaaaa
     2521 gcatttataa tcaaagcagg aacaattata tttgttgctt gtggagtaat ctggttctta
     2581 caatcattta attggtcatt acaaatggta gatgctggag atagtatatt agctagctta
     2641 ggaaatatag ttgcaccaat atttgctcct cttggatttg gaaattggca atcatcagta
     2701 gcaacagtta caggtctagt agctaaggaa aatgtagttg gaacatttgg agttttattt
     2761 ggtatatcag atgcaacaga gcaagaccca acacttcttg cttcagttgc aagtatgttt
     2821 acagtagcta gtgcgtttgc atttatggca tttaatatgc tttgtgcacc ttgttttgca
     2881 gctattggag ccataaaaag agaaatgggt tcatggaaat ggacatggat tacattagga
     2941 ttccaaactt taacagcgta tataattgct cttcttataa atcaagttgg aagtttagtt
     3001 ttaggtactg gaggaagtat tgctggggca ataatatcta tatttatagc agtagcagta
     3061 gtctttgtag ttttaactta ttcaaataaa aatatgaaaa aagaaaaaat gggtaaatta
     3121 agttatatga aaaattaatt catataaata aggagtgatg aatatggcta catttattat
     3181 agcagctatt gtagtagtat taatggcact agctgtaatg tatatggtta aaaattcaaa
     3241 aaagaatggt agcagttgtg gatgtggttg ttctggatgc tcaagtagta agagttgtca
     3301 tagcgctaag agataaatag gataagagcc tgataaaaag ttaaattgct ttattatcag
     3361 gttcttattt tttatataaa taacaaattt catatttgaa gtaggttaga ctatgctgta
     3421 attttactgt ataattatat ataaatatat gattaggagg gattattgct aaattaagca
     3481 atataagtga atattatgtt taaatttgat gttacaaacg agcttatgtg tatagcaagg
     3541 ggaagtggta aattttttgc aaaaaaaggt gctatggtag cctttaaagg aaattttaat
     3601 tttgaaaaat tgctgttagg tccaagtaat ggcggtggat tgggaagagc attattaggt
     3661 catgttcaga ggtcattgac aggagaacaa atgccaataa tggtggttga aggtgaaggt
     3721 gaaatttacc tagctcaaaa tgcttaccat gtagatgtaa tatcaattga cccaggtgat
     3781 tccatttatg ttgagagtga aaacttattg gcattttctg aacaattaaa ttacagtgta
     3841 aaattgattg gttcaggggt aatatctcaa aaaggtcttt ttactacaca tttagtaaat
     3901 aaaacaggtc aaaatcaaga tgttgctata ataacagatg gtaatcctct aattcttgaa
     3961 ggaccttgtt gtgttgaccc agatgcatta gtagcttgga ctggaagaga accatatcct
     4021 aaagtatcaa aactatcatg gaagactttt attggtcaga cttcaggaga atcttatcat
     4081 atggaatttg ttgaacctgg acaaatagtc attatacaac catctgaaag attgtctggc
     4141 ttaaaaacag attctacacc ttctgataga atgagttcag gtggattaag acttggcata
     4201 gattaaatct tatgatatag cttaattatt atttaagata ttaaaaaata aaaagggtag
     4261 cagggataga tatgttaaaa ggagttttat atgtttgaga aaattcataa aaggtttaaa
     4321 ggatattata tttttttaat aattttaatt ttatggaaga tagtatgtgt tttgggtata
     4381 tggagtagct atattctgcc tcctcctgaa atagtcttag atacttttat aaaaatgata
     4441 tatgacggtt ctattttctt aaatgtttat gccagtgtaa gaaggatatt aataggtttt
     4501 ttaataagtt cattgatggc tataccactt ggagttgtat ttggagtaaa taaaaaaatg
     4561 tatgaatatt tcaaaccact atttgaattt atgcgaagta cacctccact agctttggtg
     4621 ccaatgctta ttttatggtt tggtataggt gaagaatcta aaattataat tattattttg
     4681 gcatcctttt ttccaatatt tttaaataca ttgaaaggta taaaaaattg tgacaataaa
     4741 ttaatagaag taggtaaatc ttttaattta tctaaaaaga gaatttttta taaaataata
     4801 atccctaatt ctatgctgga tattgttgtt ggacttagtt tagggcttgg gtatagtttt
     4861 agggctataa ttggagcaga gcttattgca gcttcttcag gactaggata tttgatttct
     4921 gatggaaaag atatgtctag aactgatgtt gtattggtag gaatttttgt tattggattt
     4981 ttaggtatac tttcagatta tctattttca aagataataa caaaatttag caaagggaag
     5041 caggttgatt taaatggtta gaagtggatt tttgctagag aatatatata agaaatattt
     5101 agttgataat aaagagcatt tagttcttga taatatatct ttaaatatat ctagtgaaga
     5161 gataactgtt atattaggtg aaagtggatg tgggaaaaca acgctactta gaattttagc
     5221 agggcttgaa aatgctacta gtggaaacat atattttttt aataatgata aaaagtgtac
     5281 tccaaaagta ggaatggtat ttcaagaaag tagacttatg ccatggctga atgtaagtga
     5341 aaatatactt ttacatactg aaaaagataa tagaaataaa gttgatttag acaagtattt
     5401 aaaaatgatg aagcttgaaa agtttaaaaa ctcatatcca aatgaattat caggaggaat
     5461 ggctcacaga gtaagtatag caagagcact ttccttcaat cccgatattt tgctgatgga
     5521 cgaaccattt gcagcgctag attacttcac aagaagaaaa atgcaaaaag aagttgtaaa
     5581 tatccataaa aatactaaaa aaggtgtagt atttgtaact cacaatattg aagaagctat
     5641 ggaaatagca aagaaaataa ttgtttttag taaaaataaa aggattaaac aatttagtgt
     5701 cgaagatgaa tataatagag atttaactaa aaactattat ataaacttga aaaaagaaat
     5761 attaagggaa ttgggggagt tttaattgat taaaagaaac aaaaagattt tagcagtaat
     5821 gacatcttta ataatggtgt tggttgggac tgtaggatgt tcaacaacaa atgataagaa
     5881 agatgaacct gcaaaaaaag aggctagttt acctaaggaa ataaatttaa cttatgtaaa
     5941 atcaccactt aatgttccta gtatattaga aaaaaaggat gatttatttg gaaaggaatt
     6001 taaaaaggac aatataaaag tcaacttcca tgaattgaca acaggaccag agcaaactca
     6061 agcattagca gcaggtgagc tagacttttt acatgcatta ggaggaacgt ctgcaataat
     6121 agctgcatca aatggtgttg gtcttaaaat tacgaatatt tatagccgtt caccaaaagg
     6181 atatatgctt ataactaata atgataatat taaatctata aaagacttaa aaggtaaaaa
     6241 aattgtaggt ccaaaaggga ctatacttca tcaactgttg attgctgcac ttgctaaaga
     6301 aggctatact atagatgatg ttgaatatgt aaatatggat attcctagtg ctgcttctgc
     6361 attagaaagt aaaagtgctg atgtagcaat gcttgcaggt ccagtggcat tgaaaacgat
     6421 aaattcaggt gccaaaatga ttgtaaatgg agaaggatta gtaagtggaa taatagttac
     6481 tgctgtaagt gatgactttg ctgaaaaata tcctgaatta gtaaaaagat ttatgaaggt
     6541 tcatgaagaa actttaaaat atatgaatga aaacaaggat gaagtgatgg atgttgtatc
     6601 taaagaagtt ggtttatctt tagatgaaac aaaagaaatg tattcttggt atgactttag
     6661 ttctaaaata acggacaagg atataaaaga attagaagat acacaggaat ttttgatgtc
     6721 taatggaatg caacaaaaga aaataaatat taaagatatg ttgtacaatc aaaattagaa
     6781 tactttaaaa tattaaagtg gaaaatacac ccttatttat tatggaaact gtatggtaaa
     6841 agagggtgta tttttattat tatacaaaaa tacaattaaa aacaacttac tattttatct
     6901 acacttatgt agtctagtaa ttttctcatt ttcttacggt gaagtaaaaa ctttgaatat
     6961 gtgtcctcgg attgttttgt gtcaccataa actttccaat atttaatcat agaaacttca
     7021 taattactat atcccataaa tccaatcaca tctttaagag gatatcctaa aaaaatacct
     7081 atttcatcag gaaaaacatc actcttaagt ttatttacta aatgctctaa ataagcatta
     7141 acattcgtat tttgtttgta tcctaaaaac tttaaaaagt ttactgtttt tttacatttt
     7201 aatttttctg ataatgcttt tttatttaca aataagattt ttaatccctt tttttcttta
     7261 tcaataacta taaaatctat tttcttacat ttatatagat atttataaat atcattaatt
     7321 ttttcctctc tatttacatc agtaaacgaa atatttatta tttcagcagg tttagagcct
     7381 aaaaagactg gacctaaaac ctctaaaagc cattttatat atgatgattc aattttattt
     7441 ttacaacaaa attgtgaatt acaacaagac ataaaagata acctccttca aatgaaaatt
     7501 aatatcaagt aattagatta tactatgaat gataatcaat atcaataata aaattataat
     7561 ttttttattt aattatttat tcattatgat ttccaaaaaa ataaattata acttgtttaa
     7621 ttttagtaaa taaaaaataa aaatatagaa atactaaatt aagaaagaaa attattaaaa
     7681 ttttttgtag tacaaaacat ctaggagggt aataaaaatg aaattaaaaa gaactttatt
     7741 tgtaggatta gcattagtat tagctatagg aataactgca tgtgctaggg ataatgaaaa
     7801 accaaatgaa aatgcaggaa aaaatcctaa tgaatctgta aataataatg aaggatatat
     7861 gaattattat tcaacaagtt ataatgatta tatagcaggt ctaaatagat attctattta
     7921 tgatactcca gaaagtataa acaatcagtt taaagataaa gaatatccag gaaatgaaaa
     7981 atatctaagt gatgtaaagg ctgcatataa agatagtaga gataaaatac aatcatttgt
     8041 aacatctctt aaaaaagacg gtaaaacaga agatacagag ttaaagagaa tgaatgatga
     8101 cctaatagct gaaggggaaa gattagtaaa agatatagat gagagaatag aaaaattaga
     8161 taaggtatct gatgaagata tgaaaaaagg tcaaaatgat tttatgaaac tagttcatgg
     8221 aactgaagat gttggaaacg atattggtag tggttttaga aaaatgataa aagatatgaa
     8281 tgatagattg ggaataacag gaaataataa aaaataatag agtaaatagt tttaaagtta
     8341 gaaataaata tttgaaatat agcgaagcct tgtaattaat atattttttg gcttcgcttt
     8401 tttgtatgtt atcaagatac taatttttat cttgtaacct catattttca ttatagttta
     8461 tttagaggtt atttttttat gtttttactt gttttttctc tttttaaatt tttgttttgc
     8521 aaaattgtaa gattcttcta ttaaagtttt aaagttctca aatgtctttt cagtagggct
     8581 taggacgcat atccatccca tccatgagta gataggatgt ggcatgattg tgtccaattt
     8641 tgtaaaatca aaatccatat caactatttg ccctacacct ggacgctttg gaatatatcc
     8701 aaacatttca ataaaagttt ctttttttaa tcctatattc acacggtata catttggacg
     8761 actaaccaac gaacctttat catgattact gtctttttct ttaatggtaa gaacatatac
     8821 ccctcgcttt aaaacaccat taggattgta gtaaattgct cgttcccccc aactatccat
     8881 tagtacaaca tcatctaagt ttgatagaca ataattgaga atatcatcag ccttaatagt
     8941 atcattttcc ataaaatatc aatcccttct ttctttaata ttattattgc tttagatgca
     9001 agaatatctt atcatataat ggtgacaatt attgtcacta tatttaaatt aggagtatga
     9061 atatgtcaag agctgagcgt ttaattgaac tgatgataac aataaatgca aaaagaagtt
     9121 ttacagcggg agagctagca gaagaatttt ctgtatcaaa acgtactata ctaagagatt
     9181 tacaggtatt ggaaagtgtt ggatttccac tatattcaaa agttggagcg gctggaggat
     9241 atcatgtact taaagaacgt atacttccac caattacatt ttcagagagt gaggtaaaat
     9301 ctatattctt tgcatatcag tcactagagt attataatga tttaccattt gagcaagaaa
     9361 ctatttctgt tttaaagaaa tttttaaatt gtataccaaa tgatattcaa tataatattg
     9421 agaatatgag acgtaaactt gttttttgga caccagatag gcattgctca acaccattat
     9481 tgaaggaatt gtttaatata gtaatgaatg aatttacaat taaaattgaa tattcatcaa
     9541 agcaaaaaaa tagtatacgt accattgttc ctattggttt gtatgctatg aatggacttt
     9601 ggtattgtcc agcttactgt ataaaatcag aatctgttaa ggaatttcgt gcagacagaa
     9661 ttgtgaagat tttgagtata gaaaacttat caaataagaa atataaggtt ttatcttcta
     9721 tacatgatta tcttaagaat atggaagtag gtacagatta tcatattaaa attaatctga
     9781 cagatgaggg agtaaaacgt tgtgaaacag aatttttgtt ggcaagagga ttgaaaattt
     9841 tgtctaaggg tggatacata gatatgtata taccaaaatc tacacttaat tgggtggctg
     9901 aatatttttt aacttttgga aaaaatgcta caataataga acctatagaa cttaaacatt
     9961 tgattaaaaa atagctacgc acacttgtga ctttagtcat gagttagtag ctaaattagt
    10021 cagcatatag ggaaacttat atgtagagat aggataa
//
LOCUS       DAEPLF010000129        10052 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEPLF010000129 DAEPLF010000000
VERSION     DAEPLF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10052
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.129"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            207..857
                     /locus_tag="KR314_002550"
     CDS             207..857
                     /locus_tag="KR314_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0328925.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            1135..3201
                     /locus_tag="KR314_002551"
     CDS             1135..3201
                     /locus_tag="KR314_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBH0328926.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYVWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYIYDSNINKLKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNICILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSSMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            3218..5173
                     /locus_tag="KR314_002552"
     CDS             3218..5173
                     /locus_tag="KR314_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBH0328927.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKEAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDR
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLERSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            5189..6379
                     /locus_tag="KR314_002553"
     CDS             5189..6379
                     /locus_tag="KR314_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBH0328928.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(7227..7410)
                     /locus_tag="KR314_002554"
                     /pseudo
     CDS             complement(7227..7410)
                     /locus_tag="KR314_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427897.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7513..7887
                     /locus_tag="KR314_002555"
     CDS             7513..7887
                     /locus_tag="KR314_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0328929.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFVSTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMGILYEED"
     gene            7889..8746
                     /locus_tag="KR314_002556"
     CDS             7889..8746
                     /locus_tag="KR314_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328930.1"
                     /translation="MENILEVYNLNKTYTNFSLSNISFVLPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMSEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            8749..9387
                     /locus_tag="KR314_002557"
     CDS             8749..9387
                     /locus_tag="KR314_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBH0328931.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
BASE COUNT         3702 a         1168 c         1799 g         3383 t
ORIGIN      
        1 acgatttaat ctatagtaca ataacttgat ttgcaataca atgatttaat ctatagtata
       61 atagcatgat ttacagtaca ataatttaaa ttgtagtaca aaagtactat caaataagaa
      121 tttctacttt aaaaatatta ctaaaagtaa ttaaaatcca ataattcaat ataatatggc
      181 aaaagaatag aaataaggtg agagtagtga aagtaaagtc aaatttagat gaaattttat
      241 atcataagat tattgaaagt cttattcgtg gtgaatactc tgtaggtcaa aaaatattac
      301 taaatgacct ttgtgaaaaa tttgaagtaa gcagaacacc agttgtacaa gcagtaaaaa
      361 tgctaaataa agatggagtg cttactatta tgacaaatgg taaagtgtac gtaccagagt
      421 atgaatatga tatggtaaaa caagtttgtg aaacgagaac actgattgaa acatatgctt
      481 tagaaaaaat gatgcaagaa gaggaagagg tttttcaaca aaagctagat gtaataaaaa
      541 gatactctga taaatgcgag gagtactatc gtcaagaaaa atctgtagaa cttgcactag
      601 cagatttgga attacataaa gccattgtag aaggggcaaa taatgagatt ttaaatgatg
      661 tatatgttgg tatacaagga agatttatag tagtgaacta tttaattcga ccattaaaaa
      721 atcgtaatta tgaaggaaca gtacaagacc attttgaaat attaaaattc attgaagaca
      781 gagatacaaa aaaagcagta gataaactaa gaaatcatat tcaaggaact attaaaagat
      841 tctgtgaaga tgaataataa gttttgaagt tactatatac ttatctatta agttgctaaa
      901 tgtagtctaa gtataatcta attatagttt aaacataatc taaatataat ctaaatatag
      961 tgtaaataag tctaagtatg aattaaatat agattaagta tgaattaagc atggattaaa
     1021 tatactttaa attacaaata ttacaaggaa gccagaaggc ttcctaaaaa aataaccttt
     1081 ataaaatttt ttattttaaa tttaaaattt tttattttaa atttaaaata aaaaatgaaa
     1141 gaggtggtag agagtttgaa aaaaattact aaagaaagga tagaaaatac tatgaaaaag
     1201 ctatttacga gcgaaacatt tttggaatat caatttataa gtgattgcca aatatcacca
     1261 gatggaggat atacagcatt tattgtaaaa aaagctgata tcaaagagaa tggttacaca
     1321 agccaagttt atatattaaa caataaaact ggcgaattaa aacaaataac gtctataaac
     1381 tctgttggag catatgtctg ggaagatgaa aatactatct tgttccctgc tttgcgaaat
     1441 gaaaaagtaa aagaagctgt caaaaacggt aagcaatgca tgagttacta tgctttatct
     1501 ttaaatggtg gagaagcaga agaacttttt agactcccta tcaaaggtgg taaattgaat
     1561 cctataggaa aaggtttgta tgcagttatt gattcttatg acaatgaccg acctattgta
     1621 gagggattgc cagaagaaga acaacagaaa aaaataaatg cttataacaa aagacattat
     1681 gaacatttca aagaaattcc ttatgctgtc aatggggagg gatatattag cagaaaaaga
     1741 aaacgcctat atatatacga cagtaatata aataagttga aggcaattac agcacctatg
     1801 tttaatgttg taggtatgaa aataagtgat ggaaaaatct tatatatagg gcaagagttt
     1861 aaagatgtaa aaggtttgaa aaatggggtc tatgtatttg atacaaacac aaacacaaat
     1921 atctgtatat tagaaaaaga taagtatatt ataaaaggtt ttgaattata tcaaaatcaa
     1981 gtgatactaa acctaactga tgctttatct tatggaaatg gagaaaatgg agatttttat
     2041 actattgata ttgatacgaa agaaatgaaa cttctttcag ggcatcaaca tcattgtatt
     2101 ggaaatacag taacatctga tgttaaaatg ggtgctggtc agacaacaaa ggtagatgga
     2161 gaatatattt attacacttc tacagtagat atggactgca ttattgaaag aatacatatt
     2221 ccaacaggaa aacaggaaaa agtaacacag acaggttcag ttgattttat agatgtaaaa
     2281 gatggcaata ttgtatgtgt gggttgttta ggaaatggat taccagaagt ttatacagta
     2341 gagaatggaa cattgtgtaa gaaaacacat ttaaatgacc atattttaga ggaatataaa
     2401 atttctgtgc cagaatacat agagtcaaaa ggaagttcta aatgggaaat acaaggatac
     2461 gtatataaac cagtagatta tgaggtgggg aaaaaatatc cagctgtatt ggcgattcat
     2521 ggaggaccaa gattgacata tggaccatat tttatgcatg agatacaggt atttacaagt
     2581 gcaggttact ttgtattctt ctgtaaccca agggggtctg aaggtcgtgg caatgctttt
     2641 gcagatatta ggaaacagtt tggagatatt gattatatag attttatgga atttacagat
     2701 actgtacttg aaaaatatac agacattgat aaaacaaaat tggctgttga aggtggaagt
     2761 tatggtggat ttatgacgaa ttggataatt ggtcatacaa accgttttgc tgttgcttgt
     2821 gctcagcgtt ctattgcaaa ctggagtagc atggagggta caacagacat tggttattat
     2881 ttctgcaaag gacaaacagg tgcttctcat atggaaaacc acgagcttca atggaaacag
     2941 tcacctcttg cttatgctga taaatgtgtt acaccgactt tatttttaca tggtgaaaaa
     3001 gactatagat gctatatgca agaagcattc cagatgtttt cagcattgaa aattcatggg
     3061 tgtcctacaa aactttgtct atttgaaggg gaaaaccatg aactgtctcg ttctggtaga
     3121 ccaaaacaaa agctacaaag attagttgaa atgctagatt ggttttctgt gtacataaaa
     3181 aaagataaca taggacagta gaaaggagaa tataaaaatg gaaaaaataa agattgaaag
     3241 ttcagatatt tttgaatata tattccctca tgatatttct tgttcacctg atggaaagca
     3301 tattgcttat attatatcaa atatcaacga ggaaaaagac tgctatgaac atgacttata
     3361 tgtgatggac atcaaaacag aaaaacaaat acatatgact caaacaaaag atgtaacatc
     3421 atttagttgg atttctaata cagaactact ttttacatct aaaagaaata agccaaaagc
     3481 aggtacaaca gatttttaca ctatttctat agaaggagga gaagcaaaag aagcattttc
     3541 tattccaaaa gcttgtagcg tacctgtatc acttggaaat aaattgtggt tattgacaac
     3601 aaaaaatcca acggattcta aaaaatcaga accagatagg gcagtagagg gtgtagatta
     3661 ttggacattt acagataaac cttttataag agatagagaa aatttttctc aaagaagacg
     3721 tgtaacatta gaattatatc aagaaggtga aaatataaca aaagccatta caccaaaatt
     3781 ttgtgaagta gcaggtatag acgtttcttc tgataaaaac agaattttat atactggtca
     3841 gatttatgaa gattgtgcaa ctcctttttc tggactttgg gagtatcata tagacagtgg
     3901 tgaaaccaaa gaacttgtac cacaaggaaa gtaccaaata agtttggcaa aatatattgg
     3961 caaagataaa gtaatgttgc aagcttcaac attagaacgt tctatcacac agaatcatga
     4021 tatttttata cttgatttga gtactagtga aataaatatg attgctagtc cagatggtat
     4081 gtatgctaca ttactggatg tagatgcagt atatggtggt ggacgtagta ataaagttat
     4141 aggagataaa tttattggtg caagaatttg cagaactatg acagaattta atgagtttga
     4201 tacaaagaca ggtaatatac gtatcatcac aaaagtagat gcttttacat cttttgatat
     4261 ttacgacaac accatgtaca cagtaatgtt gaaagattat gagttagcag aaatatatag
     4321 cattgatatg actacaggta caatgaaaaa aatgactgct ttttctaaac catatttaga
     4381 tactcataag gtttcattac ctgaaaaatt gacgtttgta gcaaaaaata aagaagaagt
     4441 tgatggtttt gttataccac ctatagatgc taaagaaggc gaaaaatatc ctgctgtatt
     4501 gttcattcat ggtggaccta aatgggcata tggctatatg tttacacact tgaagcagtg
     4561 tgtaacatca aagggaatgt atgtgattta ttgtaaccct catggtggtg acggttatgg
     4621 tgaaaaattc ttagaaatgg tagaaagatg ggggtatgtt gactatgagc atcttatgga
     4681 atttgtagat acttgtattg aaaaatatcc tgggattgat gcagaccgct taggggttgc
     4741 agggggaagt tacggtggtt atatgacaaa ctggataatt ggccatacag accgtttcaa
     4801 agcagcagtt tctcaaagag gtattagtaa cttgattaca gcttctttaa ttattgactt
     4861 tggggataga attatgaagc agacttgtgg agataagacg ccttggaatc atgaggaggt
     4921 tctttggaat cattcaccta ttaaatatgt gaaaaatgtt aagacaccaa cattgttctt
     4981 acatagtgat agggattata gatgttttat gggggatact ttccaaatgt ttacagcatt
     5041 aaaacagctt ggtgtagata cagaaatgta tttgttccat ggagatacac atggattgtc
     5101 tagaaatgga agaccatcta atagaattgc acgagcaaat gctattgtag attggtttga
     5161 aagatattta taataagggg gttattctat gagtgatatg ttattatggt ttagtgtcgt
     5221 attagtttta tttgctattg gggatttaat tgcaagcaaa acaaaagcaa aagtatcagc
     5281 tgtatttgtt acactacttt tatttttgat tctatttgtc acaaaagcaa taccagctga
     5341 tattattgaa aaagcaggta tgaccgcagc agctagttgg agtgttccaa tgattatgtt
     5401 tagtatggga acaatgttga atgttaaaca atttatagac gaatggagaa cagtattgac
     5461 agcatggctt ggaattgtag cagttatagt ctgtgtctca ttatgtattc ctttgtttgg
     5521 aaaatcaaca gtattgacaa gcataccagt tataaatgga gctttacctg ctactactat
     5581 tatgacacaa gcagcactag aaaaaggact gacacttgca gcagctacag caacagttgt
     5641 atttgcaatt cagaagttta ttggtacacc aatagcttca agagcagcgt tacaagaggc
     5701 aaaccgtctt ttagtagaat atcatgaagc aaaatcaaaa ggcattgatt tagcaaatgt
     5761 tgatactgat aagaaagaag cagagaatac aggcacaaaa gtaaaacaag ctttctgtga
     5821 aaaatacgat aaatattatt ctacaaatgt ttgtatattt tttatagcac tattctctta
     5881 tctagggtat gaactttcag agattattca tgtaaattat tctattgtat gtcttgttgt
     5941 tggtgttatt gtaacacgta ttggtattgt accaaaagac attttggaaa aaggaaaaat
     6001 caagggattt attaacatgg ttgtgtttgc agctgttatt ccttctttag caaaagtttc
     6061 tttgacagat ttaattagtc tttttgtacc aattgtgggt atgtttgcag cttctatcat
     6121 tggtatattc cttatgatga aggtgttacc aggttggaaa attatcggtt ctaaacctct
     6181 tgcttttgga gtagggtttt gtcagatgtt gggattccca actacatatt taatttcaaa
     6241 tgaagtgtgt aatgctgttg gagaaacgga agaagaaaga gcatatttga tgtctaagat
     6301 tatgcctaag ctggtagttg gtggtatggc ttgtatgatt tctattgtag tagctggtct
     6361 tatggtacca atgttgtaga taggtttcat agttaaaaag tggtgttaat ttataggaga
     6421 agctaaaaag tagtattaat ttataaaata attttatatt agttaatttg ttataagaat
     6481 attttctttt tatgattata attttgattt tataagagag cagatttttt atttgctatg
     6541 ctaatagtta taatttgttt ttatttgcta aaatgcaact ttaatttgat ttatgtataa
     6601 tatagggtat ttaataatat ttttgaagaa tttttataaa aaattaaaaa gaggagaaaa
     6661 aagggagtgc cacaaagtga actttttagt tctaaggcac tcttttttgt acgaaatcga
     6721 atatattttc aacgttttaa aagtgagaaa gagacttatc tctagtttgg gatagccaaa
     6781 tttaattatt aaaattattt tttgttttat ttatcaaaga gtattcgtgt tttagaagtt
     6841 agtatttttt attttatata tccaaggttt tccgtttcta atgttttctt ttttaaaata
     6901 attagggtat tcaattatag taaaaggtgg atggtatttt ggaatgatat tatttaaagc
     6961 acagtattga atgttgaaat cctcacattt tagagtatta tcttttgtaa tcaaaaaaat
     7021 cttctatact tatagcaagg ttacttctgc aaatattcca tgcatcataa tatccaaatt
     7081 taaatttttt taatatagag tttacctcta gtctacactc ttgaataact ctttcctcta
     7141 tccaaattct tataaactca tcccatttat tactatctat ttattactat ctaaattttt
     7201 tagtatgttt gtaattggaa atggatttat tcctttatgt tttttataaa tagtttataa
     7261 cttaatttat tattaaacaa atcattcata cagaattact ttttctttta gatattcaat
     7321 tcttactttt aataaatatt taatacattc tatcttatgt ggttttaaaa agtgtttatg
     7381 gctatcaata atctctaata tttcattcat atttattttg tcatagtaat attttacatc
     7441 ttttatattg acaaaaaagt gctctaagtg tatagtgtgt atataataac atcacactgg
     7501 aggtgataaa aaatgaaagt gctcatttct aacacatcag ataagccatt ataccaacaa
     7561 attaaagagc aaattaaaga agcaatattt actgaagaac tgaaagaagg ggatgcaatg
     7621 ccctcaataa gaaattttgc taatgattta aaagttagta ttttgactat tagacgagtg
     7681 tatgaagaac ttgaaaatga aggttttctt acacgccaag ttggaatagg aacatttgtt
     7741 tcaactggaa atttagaaat actaagagac tcaaagaggc gtatagttga acaaaaaatg
     7801 gcagatatga ttaaggatgc aaaagcacta ggaattacac agatagagtt acaagaaatg
     7861 atgggtattc tatatgagga ggattaatat ggaaaatatt ttagaagttt ataacttaaa
     7921 taagacttat accaattttt cattatctaa tataagcttt gtattaccag aagattgtat
     7981 aactggattt ataggtgtta atggagcagg aaaaacaaca acaattcgtt cgatattgga
     8041 tgtcgtaaag cgtgatagtg gaaaaataaa aatctttggt aaagatttcg atggaaatga
     8101 gaaggaatta aaaaatcgaa ttggagttgt atttgatgat tcatatttct atgaggagtt
     8161 gtcaatgtca gaaatgaaaa gtattattgc accttcatat gataattggt gtgaagaaga
     8221 ttatcaacaa aatatgaagc gttttaatct taatccaaaa caaaagattg atacactttc
     8281 aaaaggaatg aaaatgaaat ttgctttagc attagcatta tcgcataaag ctgagttttt
     8341 aattatggat gagcctacaa gtggtttaga cccactaatc cgtagtcaga tacttgacat
     8401 actaacagaa tatatgaaga aatgtggtcg tggtgttttc ttttctactc atataacatc
     8461 tgatttagac aaaatagcag atatgattgt tatgattgac aatggacgta ttatattaga
     8521 gcgtgaaaaa gatactctat tggatgaaca tagaaaagta aaaggggata ttcaatttct
     8581 aaatgatgaa atcaaaaaat tatttttaaa tttagaaatt acaggttatg gttttatagg
     8641 gattacctca aaaattaatg aagtgcaaaa aaatatgcca gatattttaa tagagcgtcc
     8701 tacaattgaa gatattatgc ttgcatatat tgaaaggaga gagtaattat gttgattcat
     8761 ttagttaaaa aagatattct gattgtaaaa aaatatgtat tgttgatatt gtttattgca
     8821 ataggaatcc cattatttat attatggcgt ttacctccat ttgcacaaat attgggattt
     8881 gtactatcaa ttatattttc agaatttatg ctgtgccagt atctattatt aaaggagagt
     8941 caatattcaa aagcatctgt attactttgt tcaactcctt atcctagacg tggcttagtt
     9001 caatctaagt atgttttatt tgcaatggta tttatctatt gtacattagt ttactggatt
     9061 gaaaatttac ttattccaca aataggaact tttgacttaa catatatttt gattgtttta
     9121 ttaataagtt ctattattta tggagtttat atgcctattc aatacaaatt aggatatgaa
     9181 acaacaaaat tctttttcgt gatgataatc atggcttcat catttgtatt accattaact
     9241 atagctcaca gcaattttga aattccattt atatatcagt atccatccat gatagtgaat
     9301 attgtattgt tccttttaag tttaattata ctgttagctt caatgtttat ttctattaaa
     9361 atttttagta ataaggagct tgtataaggt aagggggaat tttatatgcc acatttgcca
     9421 gattgggctg ttttactttg tatcttactt ccaatagtat ataaagcttg tcagatattt
     9481 aaaagtaaaa aatagagtac atatttatat tgccattttt attgagtgaa gttagatggt
     9541 atgaatttaa aaggttactt gaaaataaag ttacttggtg tgggagagta acatcataat
     9601 attatagatt atatgtttct tgtgaatcaa tttgtatttt ttaatagata atatccagta
     9661 attttatttt ccattgatta ttttcaatta tgagtatgat tgtgtgtatt gtataccatc
     9721 ttttaaggtg tttttaaaaa attatatcgt caaaatataa atctaaggaa tcctataaat
     9781 aaagcttttc aattttagaa aactaataaa aagtaggaag ttaataaaaa gaagctgtct
     9841 caaaatagag ataagcttct ttttcattgc tgaaatgacg aaaatataat tgatttaata
     9901 taaaaaagag tgctttatga actatttatt ttgaacagta gatacacttg cactcaattt
     9961 agttactcct aagctttatg aactaaaagt ttattttgaa gcctattttt ctaaataatc
    10021 atctatatac agtattgcgt ttcttttatt ta
//
LOCUS       DAEPLF010000130         9929 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEPLF010000130 DAEPLF010000000
VERSION     DAEPLF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.130"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(286..987)
                     /locus_tag="KR314_002558"
     CDS             complement(286..987)
                     /locus_tag="KR314_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBH0328932.1"
                     /translation="MNNSFLDKLIETKELKNSLYNVLKHNFLYHSNKIEGSTFTTEAL
                     ALLLDKNVVTGRHTLDDVQETVNSSYVFDTVIDSLKEKITHNFLRNLHSSLIFNTTLH
                     SRGMAGIYKTIPNMILGTDVSIAQPFEVEPKLDELIEWYYSQSEVSIKVIAEFHYRFE
                     LIHPFQDGNGRIGRFVMLKQMLENNLPIKIVSWDSEDLYRNSLNSCSLGNYVPLIEYL
                     SSLEDFREVYKMLWK"
     gene            complement(1432..2112)
                     /locus_tag="KR314_002559"
     CDS             complement(1432..2112)
                     /locus_tag="KR314_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0328933.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSILGILLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLVFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            complement(2109..3035)
                     /locus_tag="KR314_002560"
     CDS             complement(2109..3035)
                     /locus_tag="KR314_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328934.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNDVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVAETDFEILKKIGTIIENPYFYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     KNQIGINSIGRKQSSLEDYFFQTTGTGGIETL"
     gene            complement(3108..4025)
                     /locus_tag="KR314_002561"
     CDS             complement(3108..4025)
                     /locus_tag="KR314_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0328935.1"
                     /translation="MELFLVVISIVLFAFYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATLEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            complement(4025..4714)
                     /locus_tag="KR314_002562"
     CDS             complement(4025..4714)
                     /locus_tag="KR314_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0328936.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYFVKTAVDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNTIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            complement(5122..5427)
                     /locus_tag="KR314_002563"
     CDS             complement(5122..5427)
                     /locus_tag="KR314_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328937.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            complement(6149..6367)
                     /locus_tag="KR314_002564"
     CDS             complement(6149..6367)
                     /locus_tag="KR314_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0328938.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(6368..6775)
                     /locus_tag="KR314_002565"
     CDS             complement(6368..6775)
                     /locus_tag="KR314_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328939.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(6861..7349)
                     /locus_tag="KR314_002566"
     CDS             complement(6861..7349)
                     /locus_tag="KR314_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBH0328940.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(7529..8437)
                     /locus_tag="KR314_002567"
     CDS             complement(7529..8437)
                     /locus_tag="KR314_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBH0328941.1"
                     /translation="MTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFINDI
                     LKDAPYANRNIKPFTHIKRIIKAETDPTINYIGEKRPRLYILAEDEESNHINIEMQRA
                     LEDDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLTM
                     DGHPNIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIKR
                     ALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGVEKGKREEKFNIIIKLKNK
                     GYNLSEICDIIDDLNKSEVEKIYNQN"
     gene            complement(8988..9158)
                     /locus_tag="KR314_002568"
     CDS             complement(8988..9158)
                     /locus_tag="KR314_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328942.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(9330..9773)
                     /locus_tag="KR314_002569"
     CDS             complement(9330..9773)
                     /locus_tag="KR314_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0328943.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         3456 a         1561 c          962 g         3950 t
ORIGIN      
        1 aaaataggta ccttttatga aaattctaat gataagcatt tttctgatgt aatggtaaat
       61 tataaatgat aaaattaaaa atcactttta gttttatgaa ctgtcccaat attaagaaac
      121 aactaagata cataaaaata gttataaaga gatagttaaa ctatatccac tattttatat
      181 agttaaaata gtgggtatag tccaagctaa gaataactaa gaaatcaacg ttaaatagca
      241 tagttaaaaa tatatttata taaaaacttt ttttgctaca aacaattatt tccataacat
      301 tttatatact tctctaaaat cttctaaact tgataaatat tcaattaaag ggacataatt
      361 acctaaacta caagaattta aggagtttct atacaagtcc tcactatccc aagatacaat
      421 ttttatagga agattatttt ctaacatctg ttttagcata acaaatctac ctatgcgacc
      481 attaccatct tgaaatggat gtatcaattc aaatctataa tgaaattcag ctataacttt
      541 aatagacact tcactctgac tatagtacca ttcaattaat tcatcaagct ttggctcaac
      601 ttcaaatggt tgtgctatgc ttacatcagt tcctagtatc atattgggta tagttttata
      661 tataccagcc atccctctgg aatgtagggt tgtattgaag attaaacttg aatgtagatt
      721 ccttaagaaa ttatgtgtta ttttctcttt cagtgagtca attactgtat caaatacata
      781 actagaatta acagtttctt gtacatcatc taaagtatgt cttcctgtaa caacattctt
      841 atcgagtaaa agtgctaatg cttcagtagt aaaagtactg ccctctatct tattagaatg
      901 ataaagaaaa ttgtgtttta acacattata aagtgaattt ttaagttctt ttgtctcaat
      961 caatttatct aaaaaggaat tgttcatgtt atcacctcaa ttattttaat atttttaaat
     1021 cattataatc ttataatact tattctaata gtatgtaaag ttcagattta atttaatact
     1081 tgtagtatat atttcattgt atttattttc agtaagtctc aacttttcat ctaatttatt
     1141 agtcaataag tttagtcaat tttatagttc atataattat tatatatcac tttgtacaac
     1201 atttcattgt gatatttcat attaatctaa aaacatataa aacgcatttt tatctcttct
     1261 tagagttcat tttatgacta taaatcatta aatttataaa taagaagaaa aaatatatag
     1321 ctattattaa tattattaaa actctaataa tattaataat agctgatata atagcaaatc
     1381 attattatcc caagaagata caattaataa ctaccttcct atccacagta tctagtcaat
     1441 ttcagtaact ttatgattta gtatataata tataccaatc acagttattc caactataat
     1501 aaaaacagaa atcagactgt cttgagttgt atttgtacca gctaaaataa attgtcttaa
     1561 attgataaaa ataattgaac tgacaagggt aacaggtatt gattttctta tcattccaac
     1621 aacgaatgtc catataccta atgagcaaaa tacaataata cttttaattg ctcctggtat
     1681 ccaataagat aaaattgaaa tatgaaaatc tccaataact aaattaaaat acttatccaa
     1741 cacaacaacg aatgtaaaac aacagatata tccaactatc attgatgata tcataagagt
     1801 cgtaatcaaa agtaactttg ataaaataag ttttttacgg tcaagtggat aagagaataa
     1861 aatcaagatt gttttatttg tatattcatt tattatatat gccacattca acacagcaaa
     1921 taatataata tagtaaaaac agaaaactaa actgattgtc ctaaatgtaa catcataatc
     1981 atatattgaa gtagatttat cattcaatcc agtaaagaca aaaaacatac ataacaaaat
     2041 ccccagtatt gaaaataaaa aatatctttt tagcccaact ttttttaatt ctagtttcat
     2101 taaatgtatc atagtgtttc tatacctcct gtacctgtag tttggaagaa gtaatcttct
     2161 aaagaacttt gtttacgtcc aatgctattt ataccaattt gatttttgat aaatattttg
     2221 gatatttctt ttacatcttt tcgtaaatca tatatttcaa tacaactttc cgaagtaatt
     2281 ttaaagtctt ttattccaaa ttctttttct aacaagtatc cagcaagttc cacattatct
     2341 acatctactt caacataatc tgtttgatat ttgtgtatat ctgacataga aatttcttta
     2401 ataagtttac catcttgaat aattccaata gtgtccgcaa tttgttctac ctctgaaaga
     2461 atatgactag aaataaatat agttattcca taatctgtat ttaacttacg gaataattct
     2521 cgcatttctc ttatcccctc tgggtcgagt gcatttatag gttcatcaag aatcaataat
     2581 tctggtttag taagaattgc tctagctatt gcaagacgtt gtttcatccc taaagaatat
     2641 tttgaaactt tcttgtttga ttgctttgac aatccgacac attccaatac ctctgatata
     2701 tgttctttgt tataataacc catatattca cagtgtaaat caagattctg tttagcagtc
     2761 aaattctcat agaaataagg attttcaata atagtaccaa tcttttttaa tatttcaaaa
     2821 tcagtttctg ccactttctt actgaacata actatttctc cagaatttgg cttaacgaga
     2881 tttagcaaca ttttcattac tgtactttta cctgctccat ttggtccaac aaatccataa
     2941 atttctccct ttttcacatg aatattaaca tcattaacaa cagctttatc tttatatatt
     3001 ttctttaatt gatgggtctc tatgatatag ttcatcatac tccctccttt ctaaaaacag
     3061 tatacaaaat aaaattatct tttctctctt ttaaatctaa cttttttcta acttttcaat
     3121 tttaccgtaa aaactgtttt tacattaggt ctactattta cataaattga acctcccata
     3181 gagtttgcaa gcccttgaga aatagccaaa cctaaaccat tgcctttttt tctgtcagca
     3241 gtataagctc ttgtgaaaat atgctctttt tcagtttcag aaatacctga acctttatcc
     3301 tcaatatcta taaaaacatg tttttccttt tcatatagtg aaatccctaa aaatctacca
     3361 tctgaaccat attttatagc attatcaatt aaatttttga gtatacgttg taatgcttct
     3421 gtgtcaacat ttgcatatat aggtctatct tcaatattaa cttcaacatt aaagttttct
     3481 ttatctagta aatcataaaa ctctaaaata atctcatgac ataattgtgt tagattggat
     3541 ttttgaagat ttaatatcat atctccagat tggatttttg ccatattaaa caggttattt
     3601 attgaattaa caagctcatt tgaatttgtt tgcatttttt ccaaagttgc tctcattgaa
     3661 gaagataatt cttctttttg actttgaaga tatagcattt caatataacc ctttaatact
     3721 gtgatgggag ttcgtaaatc gtgtgatatg tttgtaaata tttgcaatat cgctttttca
     3781 cttctattat aattaatttg cctactgtaa aacttttcaa gaagtttatt tatatctact
     3841 gctgtttctt ttacaatcga aatatcagat ggtactaaaa tataattaat ttcatcatca
     3901 tttgttatat cacgaagtct tgaattgata tacttataat cactttccat tttctttgac
     3961 ttatttcgtt cttttaagta gaaagcaaat agtacaatcg aaattacaac taaaaataac
     4021 tccattattc ttcctccatt ttgtagccaa ttccccaaag agttttaata taattatttt
     4081 tttctatacc atcagacaat ttatttctta acctgttaat atgagtattt agcacatttt
     4141 catttccata ataaggttct ttccaaatag tgttgtataa ttgttccttt gaaaaagcac
     4201 gtccttgatt agtcgctagt aatttaacaa tttcaaattc tgtatgtgtt aagtttaagt
     4261 caattccatt ctttgaaact acatattttg atatgtttat aatcaaatct ttatactgaa
     4321 taaccatatc ttcagagttt cttgaactgc tatacaccgt tgcacgtcta atattagctt
     4381 taattcttgc aagcaattct acgattgaaa aaggctttgt aatataatca tctgcaccaa
     4441 gattaagccc tagcatttta tctgtgtcac tatctttcgc agtagtaata ataacaggaa
     4501 caacactcac acttcgtata tattttaata cttcaaaacc actagacttt ggtaacatca
     4561 agtccagtaa aataatatca aatgaataat ttttaaatat ttcaatagct gtatttccgt
     4621 caacggcagt tttaacaaaa taattctcac taatcagaaa atcgtatacc atttctcgaa
     4681 tattcttatc atcttcaacc aataagatgt ccattattat tcaactcctt ttcgtcagta
     4741 tacttcaaat gatatattta ttaaaaaggg ttgtcaataa ataactgctt atgatattgt
     4801 atctgcaatt tggtgatact cttctaagtt aaagttcttt tcaataagtt ttattgaaaa
     4861 aataattaaa atatagtata ctctcttatc tttatctgaa atatacataa ttttctttta
     4921 ttaacatcta caattaacta ttttatatta taaaacacct tttaactttt gctaaaaaat
     4981 attttataca taaaaaagaa accacattct agttttatac agaatatagt ttctttaaaa
     5041 ataatcaaat tgggatttta aataattaaa aacaaacttt tatttgtttt taattttcca
     5101 attttatagt agataattta gttaattagt tcttaaatgt ttatcttctt tagtaaattt
     5161 ttttcttatt aagaaatatg caactattac aacaagttct attgctaaaa atacaccata
     5221 agtagctata gaatctatgc tatctatgcc atttctaaag aaaattaaag caattcctgc
     5281 tgctataata cctacagcat ttttccctat agaattttga ccaaaaccat aaaaagaagg
     5341 gaaatttcct tttatttcaa tgaatgtcaa gattaatcca atagctataa atagtactcc
     5401 tacaacttcc ataatcatgc taggcatttt gacccctcct tattaataat taactactac
     5461 tataaaaata tttattattt ttataattat attatatatg cgattacatg tttttggaag
     5521 tatatttgga agtaatttca tatttattta acttaatttt tttgtgataa aataattcta
     5581 attttcatat agatattatt ataaattttc tagtttatgg agttattcat tctataattc
     5641 acaaatatgt attttttaat ctattaaagt attcattttc tggcttataa gcatggactt
     5701 cttatctata agcttggttt ttatttataa ttaattcatt ctctaatcta taagcttggt
     5761 ttttatttat aagtcattta ttctctaatc tataagcttg ttttttacct ataattaatt
     5821 cattctctaa tctataagct ttctttttat ttataagtca tttattctct aatctataag
     5881 cttacttctt aatctataaa tcattcattt tttaatctat tagcttgttt tttacctata
     5941 attaattcat tctctaatct ataagctttc tttttcatct ataaatcatt cattctttaa
     6001 tctatgagct tggtttttca tctataagtc atttattctc taatctataa gcttggtttt
     6061 ttacctataa ttaattcatt ctctaataca taaacttgaa ttttttatct attaattcat
     6121 tctctaatgc ataagtaata catgaaattt aaatattatt ttcttcaata aattcaaaca
     6181 actcttctac agttgtatca aataccttag caatatccat tgccagtttt aaagaaggat
     6241 tgtagcgacc attttctaaa tgtacaatag tttcacgtct tactcctact gactttgcaa
     6301 gttcaccttg tgtcataaga ttcttttctc tataggattt aatttttgtc tttagtaata
     6361 ctgacaccta attgtatatc ccctttctgt caaaatatat aaatagaatt aacttcaaca
     6421 aggattgaat aaacaaaatg actaataaag acattcctat atttaaattt gacacattta
     6481 tgtccaaagt tacaagaaat atagatacta tagctacaga aaaaaacaat acttttattg
     6541 caatatcatt aactcttgat agtatacatt ttgatgcctc atcaattaaa tcatcatact
     6601 ttttattata gacccactga gctcccatag ctaataccca aataattata ggaagatatt
     6661 caatactact tcctttaaaa aaatacttat ttactaatgt agctccaata caataagaaa
     6721 ttcccaatat ttctgaaata agtgttgatt taataccagt ttgttgttta gacatataaa
     6781 atccccctta aaaagttatg tatttataac acctcttgtt ataaatatat aacttttttg
     6841 aataaatttc aataaaaata ttattttgca acaaattctt ttattaattt tacattagat
     6901 agaggcactt tagatgtcac tccttgcaat gtcaaatctt ctatattaac ttcatttaat
     6961 tttgaacaca gttctaagtc aaatattctt tcccaactta ccttcttttc aatttgttca
     7021 tcttcataaa gctctacagt aatatccatt aaaggtaaat gccataaatc aaattctgat
     7081 aataacacat tgtcttcact tatttctatt tcaagtaaaa cacctttttt tcctttttct
     7141 aaataaccac tacatcttaa atcaggtctc ttcgtccaaa cccatatcat actatcatca
     7201 aattcatata ttctatttgt catttgacta tacatccatt tgtatggaat atcaaagtct
     7261 tctaatataa aagaataatc atttgataga tatttattgt ttttattgtt gttccaaaat
     7321 tctaaagtct gtatagtata aaatttcata tataaatcaa cccctttgtt tttctataag
     7381 aaggagatag ttcaaacttt tattttgaat catctcctta gcttatatat ctctttgtat
     7441 ctactatagt taaataacaa ggttttatat taatttaaat ttaaaagagg aagctttata
     7501 tttatctagt gccatctcaa ttatagtatt agttttgatt atagattttt tcaacctctg
     7561 atttattcaa atcatctata atatcacata tttcactcaa attatatcct ttatttttta
     7621 attttatgat gatattaaat ttctcttctc ttttcccttt ttcaacacct tcttgaaaaa
     7681 tttcttcttt aaaatttcta cttttattca taatatacgc acctctcttt aaatttaata
     7741 aatagtcctc attactgcct aattctttta atctatcaag tgctctttta ataacataat
     7801 ctgttttaga tagtattcta atatcaatat gagaaggatt atcaataagt ttaccccata
     7861 ttagaactct gtttgctata caactggatt tacgaatttt tggaagttct ataaaatata
     7921 atgcagtttt actagaataa atatttggat gcccgtccat tgttaatctc aggcaattta
     7981 cataatcttc tatgtgatta tattttacat gatttagtat atgtattcca acagtttttc
     8041 caatttcttt atactcttta ccttcttcta actttctacc atgtacacga ctaagataat
     8101 attcagctct ttccaaataa tcatcttcaa gagctctttg catctcaata tttatatgat
     8161 tactttcttc atcttcagcc aaaatataaa gtctaggtct tttctcacca atgtaattaa
     8221 ttgttgggtc ggtttcagcc ttaattattc tttttatatg agtgaacggt ttgatattcc
     8281 tattggcata tggagcatct ttgaggatat catttataaa atttgcaagt aaatcgtaat
     8341 cattatttaa agtaaacaat gatttaaata cctcatcata ctcaggctta gcatacccat
     8401 cttcagttac aacttctcca acattttcac tagtcaatat ttcttttgga attaaatcct
     8461 tgattttatc taatttttca ttagaaaaat cacctctact aagtatgtca tttaattttt
     8521 tggttcattc tctgtccttt tcttcaatta tagttcgttt atactctttt gatgttttaa
     8581 taatcttaga tttttcttct aatttttctt tcatatcaac acctcttttt tattattacc
     8641 catttcttca taccaaagtc tgaacttcta agtaaaattt gcattattga aataagaacc
     8701 ttataaactt atatatcatt actaaagtta agactttaaa gagaagcact tagattacaa
     8761 tagattttag atagctttct ttttaaaact attgttgata aatgaatgaa tataagggta
     8821 tgtattaaaa tagagatact catctttttc attgtttaag taatgaaaat atattttatt
     8881 taatacaaaa aagagtgcct tataaactaa aaagttcact tcgagacact cccttacatt
     8941 ttgtttctta aactttacaa tattacatac ttatttatta atttaatcta tgcatactta
     9001 acccaataat aaagtacata tatccaactc attaagccat gaaaaatagc ccatggaata
     9061 gatttccatg cagtataaga aataatcatt gctaaacaag aaccaaaaga aatacccttt
     9121 ttaacagttg attttatagt attattattt gaatccataa ttaatcctcc aaaattcaaa
     9181 ataaacaaat tattatatat cttatttgaa tataatacaa tataacagct tctaaataaa
     9241 ttatactctt attatacata atttataaat tataattccg ataattatat gaattaatta
     9301 attatatcta cctaaaaata ttatcctatc taaaagttat atgatagata caacatgtca
     9361 acacattgta attctccttc aaagattggt tctggataat tatctacaaa aaagttttta
     9421 attttatgtg aataaacaaa tccaaacttc tcataaaaag gaactccgct ttcagatgta
     9481 ccaacaacca ttgatgaata ttttttacta taaacatcta aaagatgata aatcatcttt
     9541 gaggcataac ccatattttg aaaatgctca tatgtagcaa tgttttttaa ttcacatatt
     9601 tctttggaaa tttcatttac aactgctata caagtaagtt ctttattatc atacaatgcg
     9661 tacatagttc ctttattgag atatttatca atcatttctt cacatgggtc ggctaacatt
     9721 aaaaggtcaa tttgactttt tttatttatt tctatagttt ttatttctat cataagttct
     9781 ccaccctcac tttattcaaa ccttaattta acaaacttct tacagtactt atttagttca
     9841 ttttgatata tatttttact ttttaaaata tagtgataat tccctagaat attaatatta
     9901 ataataaatt ataatatcag ctctagcaa
//
LOCUS       DAEPLF010000131         9882 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEPLF010000131 DAEPLF010000000
VERSION     DAEPLF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.131"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            309..500
                     /locus_tag="KR314_002570"
     CDS             309..500
                     /locus_tag="KR314_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328944.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(589..1401)
                     /locus_tag="KR314_002571"
     CDS             complement(589..1401)
                     /locus_tag="KR314_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBH0328945.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            1823..2974
                     /locus_tag="KR314_002572"
     CDS             1823..2974
                     /locus_tag="KR314_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0328946.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDDDLDKMLELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TESLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            3092..3730
                     /locus_tag="KR314_002573"
     CDS             3092..3730
                     /locus_tag="KR314_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBH0328947.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            3731..4753
                     /locus_tag="KR314_002574"
     CDS             3731..4753
                     /locus_tag="KR314_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBH0328948.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEYIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRINYKELDR
                     IDIDEL"
     gene            4743..5363
                     /locus_tag="KR314_002575"
     CDS             4743..5363
                     /locus_tag="KR314_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328949.1"
                     /translation="MNYNSNKNKYLLKDINLEDIFIDKNIVLKFLGYGNRKAPDTISE
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGIALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(5487..6173)
                     /locus_tag="KR314_002576"
     CDS             complement(5487..6173)
                     /locus_tag="KR314_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBH0328950.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGNSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(6187..6732)
                     /locus_tag="KR314_002577"
     CDS             complement(6187..6732)
                     /locus_tag="KR314_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0328951.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCIDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            6960..8207
                     /locus_tag="KR314_002578"
     CDS             6960..8207
                     /locus_tag="KR314_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH0328952.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSSGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            8294..>9882
                     /locus_tag="KR314_002579"
     CDS             8294..>9882
                     /locus_tag="KR314_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH0328953.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIKNTFSENTN
                     KTSEIEIYNKILKINTFPVEYKKNNTEYIVVLIKDITNEKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQ"
BASE COUNT         3926 a         1069 c         1549 g         3338 t
ORIGIN      
        1 acaaagaaaa aaataacttt tattaagttg taattcatta catatattac ttactgttat
       61 atcttctgag tagtttttgt gaatataatc tatagttttc tttaagtttt ctttacataa
      121 aactaagagt tggtctatgt ttcattttat caaggcatat ttattaaaaa atcaatctct
      181 atttgttaaa ataatttatg aatgatgtta gaaactgtaa tatattggat ataatataat
      241 aaacagataa actctgtatt aagaggatat atagtttatc ttaattatag gggaagtgag
      301 gatatattat ggaaagatta atggaactca tagaaagaaa atttttaaag gatgtagaac
      361 ttttagaatt agaagacata gaaaatgtaa tagaatgcaa aatgacacag ccatataatg
      421 ctcattataa tgaatactta gtaaaagtaa aaaatggaga tgaagtagaa gaatattttg
      481 tgtatttaaa acatagataa acataaaaga ggtttatatt tatttgtatg attataaaat
      541 aaatacaagc ctcttttttt gataaatata aaattataaa atttttattt atttaatttt
      601 ttcaacaaca ttagtcaata ttgaatctaa atcatcacta atttctgtaa ctccattatg
      661 agtaatatct ataatttctt tagtcatagg aagttctcct aaaagctcta agtctaaatc
      721 atctaaaaac ttttcagtag attctccttc aaataacttg atttttttag aacaatcagg
      781 acattggata taactcatat tttcaataac tccaaggaca tttatgttca tttttttagc
      841 catattaaca gcttttgata caatcataga taccaaatct tgaggtactg aaaccataac
      901 tattccactt ataggaatag attgcataac cgttaaggca acatctccag taccaggagg
      961 catatctatt aataaatagt ctaattcttc ccaaagtaca tcagtataaa attgctttac
     1021 aactcctcca agtaaaggtc ctctccaaac aacaggttca ttctcatcat ctatcataag
     1081 gtttatagac attactttta tgttatttga gttttctaca gggtaaatat aagagccatc
     1141 agaataagct tttacatttt ttacacccat aagtctagga atacttggtc cagttatatc
     1201 tgaatctaat ataccaacct tatatcccat tttgtttagc ttattagcaa gtaaagcagt
     1261 tacagttgat tttccaactc cacccttccc actcataaca ccaattattt ttccaaattt
     1321 attgttaggg ttattttcaa tagaacaatt tgattgacta ttacaatttc cctttgatgg
     1381 acatgaatta caatctgcca tatctattac ctccaaactt taatatttat tagattattt
     1441 gtaacctaca tatactattt tagtacaatt gaaccataaa gtcattattt aaaagcaact
     1501 tagaagattt tctattgatt tattcataga ctatagcaaa aagaatacaa taaaaaaatt
     1561 ataacattct tgaaaaagtg attatttcaa tttagttata gaacttagtc atataaatat
     1621 atagaaaata ttttaatata ttatacataa cgggttattt tataagttta actataaatt
     1681 aggtttatat tttataaaaa tttgttaaaa aaaacaatta aaaattgtaa agaaaaaaga
     1741 tataattaga actgtagtaa taaatgtttt attatagata atagagtttt aagtatctta
     1801 aataatattt ggaggaatat gaatggaatt aagaccaaga agtgcaaaat tagaagaatt
     1861 tgataaagta atagaattaa taaactatgt atttagaata tcaagaaatc ataagccaac
     1921 tatgatggaa gaatttcctc ttttactaag taaaaataat atagaaaata tgattataat
     1981 atcagaagat gataaagtgg tttctgatgt aaactatcta attcaagatg tatctattca
     2041 aggaaataga ttgaaggttg cagctatagg tggagtatgt actcatccag attatgaaaa
     2101 gagaggctat tcatctaaga tattggataa agtagaagaa aaaatgtttt atgatggtgt
     2161 agatattgta attatatcag gaacaagaag tctatacagt agaagaaatt gtagtttggt
     2221 aaagagtttt tataaatata caataaaacc tgaagatgta aaaatagcat atgaaatagt
     2281 tgaatttgat gaaactaatt ttgaaaaaga tgatgattta gataaaatgc ttgaactata
     2341 taatcaaaat agtactagat ttataagaac gagagatgag tttcaaaaac tattgcatgc
     2401 agcaactatt gcctggggac caattggtta caagaaggta tttataaagg aaaataataa
     2461 tataatagga tatttgataa taagaacaat aaaaaaagaa gattctactg taggagaagt
     2521 tgctgaaata gggttaaact ctgtaaatgt tgaaaatata ttgaaatatg ttgcaaataa
     2581 atttggatta gaatatttaa attataaagt acatgtgaaa aatcttaaag accagttgaa
     2641 atgtaatggg acagaatctt tggactatca acaaggaact atgaaaataa taaattttac
     2701 taagttgtgt gatagtttaa gaagttattt tagtcaatat gtagattttg aattactaaa
     2761 atacatggaa tttaaacaag ttgaaaataa atatattatt aaatacaaag aagaagaact
     2821 tgtaatagaa aatttggata agcttaataa actgtttttt gaaaaaaatg aggaacagta
     2881 taatgaattt aaacatctaa aaaatatata tgaatttgct acaaaggcat ttcctgtaga
     2941 ttttccatgg actgcaaatt taaattatca atagttttag atgatattaa ataaaattga
     3001 ttgataccta gagttagtcc ttgttaaata agagaaatta ttggatattg aaaaagcaat
     3061 tttgtaagta attagaaagg atgagtatta tatggacata cttgatgata tagcagaatg
     3121 tatattaaat atgggtgtag ataatataga gaatttagta aaaattgcta tagataaaaa
     3181 tattgatgtg gaagatattt atgaatatgg tttaaacaaa ggtatgatag gagctttaga
     3241 taagtttgag aataaggaat actatctttc agaagttata gtatgcacag atgcattaaa
     3301 taaaggtatt aatcttttga agggaactgg aaaagtaaag aaaaaatcaa agggagtaat
     3361 tctgatgtct gtagtagaag gagatactca tgagatagga aaaaatattg taaaagtaat
     3421 ggtagaagcc actggatata aggttattga cttaggtgta aatagaaaaa gtgaagatat
     3481 aatagaagaa gctataaaaa acaatgtaga tataataggc ttatcttcaa tgatgacaac
     3541 tacaatggaa aatatgaaat cggttataga cgagcttaat atgattgaga tagataaaag
     3601 accaaaagta ataattggtg gaggacctgt aagtatggag tttgcagaag aaataggagc
     3661 agatggatac agttcaaatg ctccaaaagc agttaaatta ataaacaaac ttataggagg
     3721 tgaagtttaa atgttttcag atatgacttt acttcagtat ataaatagtg aagatagagg
     3781 tttttttctg cctgatatgg gtacaaatgg attgttctta ataaataaaa aggcatatga
     3841 agtatatgat agtgtagatt tgcaattaga attagcaaag actatggaca agtattttga
     3901 atcagatttt atatactcat tctgtgatgg gattacattt tgtgaaactc tgggtttaga
     3961 gacattaaga ccagattatg attttccaag tgtattgaca catactataa ctgacagaga
     4021 taaattaaga aaattagatg ttccagaccc ttatacgagt ggacgtatgc cactcaatat
     4081 agagagctta tcagttatat caaaaagtat taaaaaacca ctatatgaat cgatacaagg
     4141 tccatttaca cttgcaggtc aattagctgg agctactcaa cttttaagat gtataataac
     4201 tgataaaaaa tttgtagaag aattgttgga atttacaact gaattggtaa gaagatatgc
     4261 tgtagctgca aataaagcag gagcaaaata tatttcaata gcagagccaa cgtcagtaac
     4321 cctaagtaaa gatagatttg atgaatatat agtaaaaaat ttaaataaaa tctacaatga
     4381 acttgattgt tggaaaggta tgcatatatg tggagacacc agagaattat tagataatat
     4441 gctaagttgt aatatagatg cagtaagttt agaccaaatt ttagactatg aagaaattgc
     4501 tccaatgatt cctagagata tagtgttgat tggtaattta gacccaataa aacttcttgg
     4561 acgttctaag ccagataaga ttagaagaga gacactaaaa ttgttaaaga agatgagagg
     4621 atatgataac tttttatgtg attttggctg taactgctta aataccactc cagtagaaaa
     4681 tttacaagca gctataaaag caggaagaat taattataaa gaattagata ggattgatat
     4741 tgatgaatta taatagtaat aaaaataaat atcttttaaa ggatataaat ttagaggata
     4801 tttttataga taagaatatt gtgctaaagt ttttggggta tggaaataga aaagctccag
     4861 atacaattag tgaaatcgtt gaaagagaaa taagaaatat aaatgatatt ttagatatca
     4921 agatatatat aagtgaagtt gatattaata aaacacctaa agaatgtaaa aaagcatttg
     4981 ctgtactata tacaataggg gataaaattg atattaagat gaatgactat atggaaaatt
     5041 gcaatatgat ggcagggata gcattggata aaataggtat agtatgttta gattatatta
     5101 atgagaaaat aaaaatgtat ttaaaaggaa agtatgatag tcttaaaata tcgcatgaaa
     5161 tatatccagg agataaagat tttgaagtag agagacaaaa ggatatttat aactatgtaa
     5221 aaaataaata taatagtata gaaatagaaa tcaatgatta tcatcaacta tcccccataa
     5281 aaagtgttgc aatgcttatt ttaatgggag atgaagaaaa tttagaatca agatgtagca
     5341 aatgtcctag aaaatgcttt taactagttt tcaagactaa gatttgtttt gtgtatgaat
     5401 tgcaataatt tttttggtca ttttatgtat aaactaaaaa aattattgca aagtaatatt
     5461 ctatttaatt tgaacagact tacaaactat agtagtttct ttaattcaat aagtgtattt
     5521 atttcataag ttggtatatg attagtataa tttatactgt tattggaatt taaccaacaa
     5581 gtatctattc ctgaatttat tccacctaag acatcagagg ttaagctatt cccaatcatt
     5641 aaggcagaag atttatcatg acaattaaac tttttgagag cgtatttaaa tatttcagga
     5701 tttggttttg atattttcaa ctcttcagat acaaccattc catcaatata tccttttatt
     5761 tttgaatttt caagtctttt cttttgaact gcagaaattc catttgttat tataacaata
     5821 gtatactttt ttttaagata ttgtaatata tcgattgcac ctggaattaa aaatgtacat
     5881 tcacctaaaa aatttaagta cattttgcta agagtttcag aatcaacatc caaagatatt
     5941 ttatttgcaa aaagttcaaa tctaagtaat ttaagttcat tcaaagttat aatatttttt
     6001 tcaaggtcga gccaaagctt atcacttata tttctatatg actctatata attctcaaaa
     6061 ttaaattcta aatcaaactc agatagaagt tttttaaaag catgtgattc acttttctta
     6121 aaatcaaata aagtatcatc tgcatcaaaa aatataaatt tatatgtctt catggttttt
     6181 actcctttac aagatgactg caaaaattac tttactttca ctatttgagt cattttgcca
     6241 tttatgcttt gttcctaaag gaacttttac actatctcct ttggacaaag taaattcatc
     6301 atcatccata aaaagtttaa cttctccctc caaaacatat gcaatttcat ctccattatg
     6361 agtaatccta tcaatacaag aagaagtatt tggagctaaa tccataattg caaattctaa
     6421 attaccacta gagtctggaa ttaaaatctc atatataact tctttgcttc caggtaaaat
     6481 aacttttttt ctattatcat gtcttactat caagtttttt ttatctactt cagatacaaa
     6541 aaatgtaaat aaaggaacgt ttagagaaga ggctatagat tttaatgaat ttacagatgg
     6601 attagcaaga cctcgttcta tttggctcaa taatgatggt gtaacatcag ctagttttgc
     6661 taaatctcga atacttaagt tttgtttttt tcgaacttca gctatttttt cccctaaatt
     6721 taaatcattc atataaatat cctctctttt taatttaaaa aatattaact taaaataatc
     6781 aagtaaaatg attatgtagt atgaaattat tataacatat agaattaggt tttgaaaaaa
     6841 ctatgttcaa tataattaaa taaattaaaa tataattgac aaagtggata cagaatactt
     6901 aaaatatatt taaataaaat taagcaaaag atagttaatt tatgatagga ggggacatta
     6961 tgcaaataac gacttttgga gctatatttg gtttattaat agcaattatt ttaattataa
     7021 aaaaatttca agctgtatat agtttaatgt taggagcatt tattggagga ttagttggag
     7081 gagcaaatat aacacaaact gtagatttta tggcaaatgg agctatgaat atatcaccat
     7141 caattcttag agctttggca tcaggtgtat tagctggaag tttaataaaa acaggagcag
     7201 tagataaaat atcagaacag attgtaaaaa tatttggtga aaagagggct ttattttcga
     7261 ttgccatttc aactatggta cttgctggag taggcgtaaa tttggatgta tctataataa
     7321 ctgtagcacc tataggatta tatataggta gaaaattaaa ttattctaaa ttgtcaatac
     7381 tattagctat gttgggaggg ggtaaagcag gtaatataat atcaccaaat ccaaatacta
     7441 tagcagtagc agataatttt tctgtcaatc tctctagtgt tatgatggca aatataattc
     7501 cagcaattat tggagttgtt attacagtta ttttagcaag tatattaata aataaaggaa
     7561 ataaagtaca atcatatgaa attttagagc aaagagaaga tttaccaagt ttatttaaat
     7621 cattatgtgg tccaattata gctatatttc tattgttttt aggaaatgta tctcctatag
     7681 ttatagaccc aatgatagcc cttccaatag gaggaatcgt aactctaatt gtcacaggaa
     7741 acttgaacaa ttcaagagaa tatttagctt ttggattatc taagatgcaa ggggtatgca
     7801 tattattact tggaacaggg actatcgcag gaattataca gatgtctgaa ttacaacaga
     7861 gtacaatagg tgctttacaa tttttaaata tgccacaatt tttattggca ccagtttcgg
     7921 gtatacttat gtcattagca acagcatcat caacagctgg tgctactata gcatcatcta
     7981 cttttcatga tgctataata aatggtgggc tatcaccgat ttcaggtgca tcaatagtaa
     8041 atgctgggtc tagtgtattc gagcaattac cacatggctc actatttcat actagttcag
     8101 gtagtataaa catggatata ggtgaaagat ttaagttaat tccatatgaa gcattgatag
     8161 gaatagtaat gactataatt tcaacatcta ttcaattagt tttataataa atataaatat
     8221 ttaatgaaaa aagtaggatt ttgttattaa atattgaatt tattatagta aaaaagaaga
     8281 ttggagtttt aaagtgaata aaaaaaagat tgttattata gggattattt attcattttt
     8341 agtagtattt tcacttacaa atatgtatgt aaatatggag tataatttaa atgtatttga
     8401 atatattaag aagtcacttc catttacaga agaagaaaaa aagtggttag aaaaacacaa
     8461 gaatttgatt tacagctctg accaaagttc acctcctttg agatataagg gtaaggaaga
     8521 tggacagtat aagggaatta ttgttgattt aataaattct ctgtcaattc aaattggtag
     8581 agatttttat tttaaaccaa ataattggtg gaaagaatct ttcgtaaatt caatagatga
     8641 tagtataaag ttttttgact tgattccatc aaaagaaagg gcaaataaat ttatatttac
     8701 tgacccaata tacactttga gtgcaaatat attgaaagat aaaaagtccc aagatataaa
     8761 tagttacatg gatttaaagg ggaaaacagt agcaattcca gaaggtgatt attctataaa
     8821 ttttttaaag caaaaaattc aagatataaa cattttattg actccagata taaagactgg
     8881 agtcaaccat ttaatgtctg gaaaagtaga cgcagttgtt ggtgatgaac cagtattgcg
     8941 atactatata aataattatg gactttcaaa taaatacagt gttttaagta atcctatata
     9001 tactaaaaaa gcagttcttg ctgtacctaa acaatatgaa gagctggtta gtattttaaa
     9061 taaaggcatt tttaaacttc agaaaaatgg agtgtataaa gatttaaaga aaaaatggta
     9121 ttctacgtat aatgaagttg atgatatatt gtatgagcga ggaattgtgc ctagtattta
     9181 tctttttata ggtataattt taatatcaat ttatgttttt tatagctata catatttgtt
     9241 aaagatagaa atcaaaagga aaacagaaca ggtaatagaa aataaaaaaa ctttagaggc
     9301 aacttttaat agtattacag atataataat gcttgtagat gaaaataaca atatagtaga
     9361 atcgaacaaa gtactatatg actttatggg tgaaatgagt tataagatag cagatttgat
     9421 tagcatgata aaaggtgtta ttaaaaatac cttctctgaa aatactaata aaactagtga
     9481 aatagaaata tacaataaaa ttttaaagat aaacactttt cctgtagagt ataaaaaaaa
     9541 taatactgag tatatagtag ttcttattaa agatataact aatgaaaaaa tagtagaggc
     9601 gaaattactt agagaaaaca aaatgatatc tataggacaa ttagcttctg gggtagcaca
     9661 tgagataaga aatcctttgg gtataataag aaataattgc tatctattaa aagataacgt
     9721 tacaatggaa gaagtgaatg attgtgttaa atcaatagaa agtaatgtag atagggcaag
     9781 taatataata accaacttgt taaatttcgc aagaatatca gatgataatt tagaacatat
     9841 aaatataaga aattttattg aaaatatagt gaaacttcaa ta
//
LOCUS       DAEPLF010000132         9525 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEPLF010000132 DAEPLF010000000
VERSION     DAEPLF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.132"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            496..1701
                     /locus_tag="KR314_002580"
     CDS             496..1701
                     /locus_tag="KR314_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBH0328954.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVGPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNVKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1772..1993)
                     /locus_tag="KR314_002581"
     CDS             complement(1772..1993)
                     /locus_tag="KR314_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328955.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2134..2733)
                     /gene="recR"
                     /locus_tag="KR314_002582"
     CDS             complement(2134..2733)
                     /gene="recR"
                     /locus_tag="KR314_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBH0328956.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2746..3096)
                     /locus_tag="KR314_002583"
     CDS             complement(2746..3096)
                     /locus_tag="KR314_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBH0328957.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3155..4792)
                     /gene="dnaX"
                     /locus_tag="KR314_002584"
     CDS             complement(3155..4792)
                     /gene="dnaX"
                     /locus_tag="KR314_002584"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBH0328958.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKYSIDENESK"
     gene            complement(4869..5133)
                     /gene="ffs"
                     /locus_tag="KR314_002585"
     ncRNA           complement(4869..5133)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KR314_002585"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5171..5262)
                     /locus_tag="KR314_002586"
     tRNA            complement(5171..5262)
                     /locus_tag="KR314_002586"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5226..5228),aa:Ser,seq:gga)
     gene            complement(5263..5718)
                     /locus_tag="KR314_002587"
     CDS             complement(5263..5718)
                     /locus_tag="KR314_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBH0328959.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(6038..7309)
                     /gene="serS"
                     /locus_tag="KR314_002588"
     CDS             complement(6038..7309)
                     /gene="serS"
                     /locus_tag="KR314_002588"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBH0328960.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKTRLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEYKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7434..7640)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7767..8648)
                     /locus_tag="KR314_002589"
     CDS             complement(7767..8648)
                     /locus_tag="KR314_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBH0328961.1"
                     /translation="MNFLSLAKAASTNSNITKRTLENMLNNFSDNIPNIIYAIIVFVI
                     GIYISKIVRKMVSRFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGVIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFINEKR
                     ILDDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENNIKVPFVP
                     SSLLFNNMSNSNTMDKR"
     gene            complement(8660..9169)
                     /locus_tag="KR314_002590"
     CDS             complement(8660..9169)
                     /locus_tag="KR314_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBH0328962.1"
                     /translation="MNSDERRSKLIDILKESKCPVKGGMLAEILNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHRNSKEIYDELKIIVDLGGIVKDVIVNH
                     PTYGQITAELNIHSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIETLKEETIEVITK
                     ELEDRGILS"
     gene            complement(9296..9412)
                     /gene="rrf"
                     /locus_tag="KR314_002591"
     rRNA            complement(9296..9412)
                     /gene="rrf"
                     /locus_tag="KR314_002591"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3036 a         1667 c         1026 g         3796 t
ORIGIN      
        1 ctcagatact attgttatca aaatatctgg ctttatggtt tgtttcttaa tataaattaa
       61 cctactgttt aattttcaat gttcgtgttt atttcttttt ccttttttca aggacaagta
      121 atactatatc atctttatct attctagtca atataatttt ttactttttt taaatttttt
      181 atttatcatg tattctttat atttaatttt taaaacttaa tgctaagtaa acctatttat
      241 ttcataaaac taatatatat tctatgctct attttccttt ttattctata tttaaatttt
      301 ttatttacaa gaatattttt aatataacat atttgataaa attaatttac atttctttca
      361 cttgtaattt ttgcaatctt gtattagatt aatattaaaa ataatttatc aataacctta
      421 ttcacaaaga cagtttttat atatcaaaat ataatacaat ataaatacta tgctacattt
      481 taggaggatt ttattatgag caaaaaagta ttaataatgt ctgcttccac tggtggtggg
      541 cataataggg ctgctctagc tattaaagaa gaacttactt ccaaaacact tgatggagag
      601 ccaattgaat gtgaaataat agatagtttg aaattagtta ataatacaat ggataaaata
      661 atttcaagag gatacgaaaa atctgctata tatactccca aagcctatgg aagtgtatat
      721 agattatctg aaacaaatct tttgtcaaaa aatgaattta aagataatct actaataaca
      781 ttcatggcta aaaaatttaa aaagcttatt agaagcgaaa aaccagactt gattatagga
      841 acacatcctt ttccaatgat tgctctaagc acattgaaaa aaaactttaa tttacataat
      901 aatgaatcaa atgcttacac agagcatttt tataaacatt atacaaatac cattaatgtt
      961 cctccgctta tttctgtatt aaccgattat actactcatt ctacatggat acaaaatgaa
     1021 attgattact atattgttgg tcatgaatat gtaaaagaat tattggtgtt tgatggagtt
     1081 ggaccgagta aaattagaac atttggaatc cctgttgaaa aatcttttct ctctcacaga
     1141 gataaagata ttgttctttc tgagttgaat ttatctcctg ataaattaac tgttttactt
     1201 atggggggta gctttggggc tggaaatata aaagagacac ttgatgaatt actggatact
     1261 gatagagatt ttcaaatact tgtaattact ggtaagaatg aaagcctaaa agaaaaaatc
     1321 gaaaaaaaac ttatgtctcg ttatcatgat aaaaatgtat gtgttcttgg atacactgat
     1381 aagatgaatg atattttagc ttctatagat gtattagtaa gcaaacctgg tggacttact
     1441 acaactgagg ctcttttaaa agatgtacct atgatagttc cttattatat accaggtcag
     1501 gaagaagaga atcttgattt cttatctaat tgtggtgcag cacttagaac tacaaaaaaa
     1561 tataacttgt caatattact aaaagttcta atagatgacc ctagcagatt agaaatgcta
     1621 aaaagaaatg ttaaatcaat aagaaagtca aattcagccc aaaatatagc taatttaatt
     1681 ttaaatataa tttctggttg ataaaataaa taaaatccct atagtatata tattatctat
     1741 tagggatttt ttattttcta attatttaat attaaattgg ttcataaaag tcttcttcat
     1801 aatcatactg ataattttta taattatttc taaattcagc atttttattt cgtacatttt
     1861 ttttagtttt tttcttatta gaaaaactta tatcctcaaa atcatcatat ttaggtttat
     1921 cttttgattt cattttttta actctttttt ctttcattgc attatgttct tctaagttca
     1981 taattggttt catgttgcac ccctatcaaa atttttaaat gatttataca acaaattata
     2041 caatacagaa gaaatttatt caataaatct aaatcaaaat taatttgaat ttaactcaaa
     2101 aaactacaaa aatcgaggta ttaacctcga tttttatatt tctcttctgc cttctagtgc
     2161 ttttgatatt gtgacctcat ctgcatactc taaatcgcct ccaacaggaa gaccatgagc
     2221 tatccgagtg actttgattc ccattggttt tactaaccta gcaatataca tggctgtcgc
     2281 ttcaccttca atagtaggat ttgttgccat tattatttct ttgacttcct gattgccaag
     2341 tctttggata agttccttta tttttaacat atctggtcca attccatcca ttggagaaat
     2401 tactccattt aatacatgat actgtccttt aaactctctc gttctctcca tagctgctac
     2461 atctcttgga tcttccacaa cacatataac acttgaatct ctacttttat ttgaacacat
     2521 gctacatggg tctgtatctg ttatattaca acaaattgag caatatctta tttctctttt
     2581 tgcatctata atcgctttag ataaagcttc cacatcatta gtattcatat taataatatg
     2641 aaaagccaat ctttgagcag tctttcttcc tactccagga agctttgaaa attcctctat
     2701 aagtctcgtt atgggacctg tataaacttg cataaatacc acctactaga ataacccagg
     2761 tatattcata cctccagtta ccttactcat ttgacttgct tgtatatcat caatatttct
     2821 caatgcttga tttactgcac ttaaaactaa atcttgtagc atttctatat catctggatc
     2881 aactacttct ggcttgatag ttatatctat aacttctttt ttaccattaa cttttacagt
     2941 aactgctcct ccaccaactg aagcttctac ttctttactt tctaactctt gttgagcttt
     3001 ttgcatattt tcttgcattt tttgagcctg ctttaacaag ttgttcatat ttccaccacc
     3061 aggcatcatt ccgcctccaa aacctttttt agccataaac actattcctc ctaaacttat
     3121 tttcatttta ttattatatc ataatagtat tttattattt actctcattt tcatcaatac
     3181 tatattttat ttctaatatc tcttcactca ctatgttctt taatatctcc tcaccaatgt
     3241 cctttttctc tttaacttca agatttatat ctttaacttc tgatgtcagt actattttta
     3301 cattaaaact tctatttaaa acatctctta ttgttttttc taaataattt attgtatctt
     3361 gagagctcag tctagttttt gcaaaatcaa atttatcatc aaatataaca tacaacatat
     3421 tagaatatac attaaaactt tttacttcac ttaagagtgc gtatattggc atttttttat
     3481 cttttttaat ttgtttaagt atattcttcc aagatgactc tataagtata acgtcctcat
     3541 tttttacatc ttcataaact acattttctt gttttacatc tatttctcta tcgacctcat
     3601 actctttttc tttattattc ccaatttttt cagacttaaa gttccccagt tcaatcattt
     3661 tttctaagtt ctcaactctt tttataagag cttctttact ttcatcaaac attggttgag
     3721 caattttcat tatggttatt tccactaaaa cccttggatt tgaagatgac tttatatcat
     3781 cctgagttat tgataatata tttaaaatcc ttattaaatc atttatatct atatttttag
     3841 actgaatttt aagttgttct atagtttctt ctggtaatga aattatttca tccaattctg
     3901 atgaaacctt acataccatt aaatttctaa aatgatcaat taagtcattt ataagatttc
     3961 ttatatcttt gccccataag acaaattcat ttaatatctc aagagacttt ttagtgtcct
     4021 gctctacaat acattgagac aattcaaata attgttctat atttacgctt cccattaatt
     4081 caacaacatc tttataatct atcttacttt ctccaaaaga catacattgg tctaatatac
     4141 taagtgcatc tctaagagct ccttgagaat ttctagctat taaattaaga gctttatcat
     4201 ctatatcaat tccttcatct tcacaaatct ttttcattct aagggtcata tctttaactg
     4261 taactctttt aaaatcgtat ctttggcatc ttgataaaat ggttgcaggt attttgtgag
     4321 gctctgtagt tgctaatata aaaattacat atgatggagg ttcctctaag gtctttaata
     4381 atgcattaaa agctccttga gacaacatat gaacttcatc tattatatat actttatatt
     4441 tagcattggc tggagaatat ttcacacttt ctctaagctc tcttatatca tctacactat
     4501 tatttgaagc tgcatctatt tctacaacat ccattgtatt atcttttaat acagattcac
     4561 atatctcaca ctcattacac ggttcttcat ttattttatt caaacagttt actgctcttg
     4621 aaaaaatctt tgcagtagat gttttaccag tacctcttgt tccacaaaac aaataagcat
     4681 gtcctatatt atcactatat atttgatttt ttaaagtctt aataatatga tcttgaccaa
     4741 taacatcttc aaatttttgt ggtctatatg ctctatataa tgctttatgc atctttttac
     4801 ctctctttag caaaattaat aaaaaaatcc ctcattatct aagggattag tcttttatat
     4861 attaaatgct gtacaccatc tatcgactta attccatagg cgttaccaaa acagttagct
     4921 caacttaggc acccccacgg cacacaaaag tgatcactta ccgctgcttc cttccggatc
     4981 tgacggggtt catgagcttt cattgcgcag gacctaagca tcaacaccac ttatttaggg
     5041 cggccctaca gaaaataagc ctttagatgg aattcaatcc tgctatagcg gattgcaggt
     5101 tacagggcac cgctacctcc ccatctagta cagcaaaatt acaaccatat ttaattactt
     5161 tcattgtttc tggcggagag agtgggattt gaacccacga tacagtttta cccatatact
     5221 cgctttccag gcgagcgcct tcgaccactc gaccatctct ccttaatctc tttttctcaa
     5281 tgccttaaaa aattcttgca atatgatcga gcattcttct tgcaaaacat caaaagctac
     5341 ttttacatta ttattattaa aataatctaa tttaaactca tgctgttttt ctatatatga
     5401 attttttaca tgtcttgtcc ctattataag cttttttatt ctagaattaa ctattgcgcc
     5461 actacacatt atgcaaggtt ccattgtaac atataaatca caatcagtca acctccaacc
     5521 acctaatttt tctgatgctt gttttatcgc taaaatttct gcatgtgccg tagagtcttt
     5581 taacgtttct gtaaggttat gagctctagc tattatttga ttatctttaa ctataatagc
     5641 acctattggt gtttcttttt tattatatgc tttatatgct tcttttaatg cttctttcat
     5701 ataaaatgaa ctttccataa ttcctcccaa aacttataac aaacttatct tatcataatt
     5761 cattttataa ttcaacttaa ttttattttg tttatttaat tattactata tatagcgata
     5821 ttttttttaa ttagttctaa aataattttt agatattcat acaagagtgt tttataaaaa
     5881 tatttagtgt tttatgatga aaattctgct gtgtaaatta acataaaaaa actatgaaat
     5941 taatatttat aataatttca tagttttttt atttattgtt tactcataat atattctctt
     6001 catcaaagca tactctacat atacaattaa tataaattta ttttataaca tcaacaccca
     6061 tataaggtct taattcttct ggaactacta cagaaccatc tgcctgctga taattttcta
     6121 atatagcagc caaacatctt cctatcgcta atccagaacc atttaatgta tgaacatatt
     6181 ctgcttttga ttttttatct cttttaaatc ttattcctgc tcttcttgct tggaaatctt
     6241 caaaatttga acaagaagat atttctacat atctattgta acttggcatc caaacttcta
     6301 aatcatattt aaatgcagct gtaaatccca aatctcctgt acatattttt acaactctat
     6361 atggaagacc taacatttgt aacatagttt cagcatcatg agttaatgat tctaattcat
     6421 tataagaatc ttctggttta acaaatttaa ctaattcaac tttattaaat tgatgttgtc
     6481 taactaaacc tcttgtatct cttcctgctg aacctgcttc agatctaaaa caaggagtat
     6541 atgcacagta tttaattgga agctctgcaa catctaatat ttcattagca tgtatattag
     6601 taacaggaac ttcagctgtt ggtattaaga aatactctaa tccttctatt ttaaacatat
     6661 cttcttcaaa tttaggaagt tgaccagtcc ctataaaact atttctattg gccataaatg
     6721 gaggtaagac ttctgtatag ccatgcttag ctgtatgagt atttaaaaag aaattcatta
     6781 aagatctttc taatctagct cctaatcccc tatataaagt aaaccttgac ccagttattt
     6841 tccctgctct ttcaaaatct aaaataccta gacctgtccc tatatcccaa tgcgctttat
     6901 actcaaaatc aaatttagta ggttctcccc aagttcttat ttgaacattg tcttcatctg
     6961 tttcaccttg tggtacctct ggatgaggaa catttggtat tctcattaat gtatattcca
     7021 tttttgcttc tacttctttt actttctcat caataccttt tatcttatct gataactctt
     7081 tcaatcttgt cttttcttct gttacatctt ttccttcttt tattaattga ggtatttttt
     7141 tagaatttac atttaactca tttttcataa cttcaacttc ttgaagtatt tctcttcttt
     7201 tattatctag ttccactaca gcatcaaggt caaattctct ttcacctctt ctttccatag
     7261 cttttttgat atcatctaga ttttctctta ttcttttaat atctaacata ttaattcctc
     7321 ctaataattt tgataatata aattataatt ccaattaatt tacaagtatt ctagctgtaa
     7381 acttttaatt taaacaaatt tgaaaaataa aaaaagacct ccgtttaccc tataaaaggg
     7441 acgaaaatct tttatccgcg ttgccaccct aattgattat actaaaacgt ataatcctct
     7501 cagacctata acgtaggtaa accgtctagc tttaactaga agctctagag tagattcaaa
     7561 aaccattgta atgagttctc accatccact cattctctga atacaatctg ttttttacta
     7621 ttttccttca ccgcttttta actaattgtt ttatttgata atcattatat tatatatatt
     7681 aacatctttc aatactatta ttcaaatttt tttagaattt tctttaattt tttatattaa
     7741 attcactttt ttacttaatt gaaattctat cttttatcca ttgtatttga attgctcata
     7801 ttattaaata ataacgagga aggaacaaat ggaactttta tattattttc gtcaaattta
     7861 tttttgactt tttccattac atcaaagtaa acatcccagt aatcatcagt tttaacccat
     7921 ggtctagcaa ctatttgcat agtgttgttt cctatagagt ttaaacctat aattggtttt
     7981 gggtcatcca atatcctttt ttcatttata aaaatttcat tcaatatgga cttaactaca
     8041 tcaatatcac tatcatatga aatattatat gaaaaatcta tccttctttt attttgatgt
     8101 gtgtaattaa ttatattgtt acttgtcaat tgaaagtttg gtataattat ggttttatta
     8161 tctggggttt taagaacagt actaaatatt tgtatatcag aaactgtgcc ttctacacct
     8221 gagccttgaa tataatcacc tatattaaat ggcttaaaaa ataaaataat catacctgaa
     8281 cctaaatttg ataatatttc tttaaatgct aaacctatac taaatccagc agctcctaat
     8341 acagctacaa ctgatgtagt ttgtatccct ataacaccca agcttatcaa tgatataata
     8401 gataacatag atatgtatat tccatataca atgaaattat ttacaccttt actcattcca
     8461 tatttgatta aaaaccttga aaccatcttt cttactatct tagatatata gatacctatt
     8521 acaaatacga ttattgcata tataatattt ggtatattat cactaaagtt attaagcata
     8581 ttttctaaag ttcttttagt tatattacta tttgttgaag cagccttagc taatgataaa
     8641 aaattcattt aactcctcct tatgataata ttcctctgtc ctctaattct tttgttataa
     8701 cttctatagt ctcttctttt aatgtctcaa ttgtatgaat atgactacat tcagttagaa
     8761 ctgataactg tttaaattca tttgtctcaa ccttcttcat aaaattatct atatccattt
     8821 tagagtgaat attaagctct gcagttatct gaccatatgt tggatgattt acgatgacat
     8881 ccttaacaat tccacctaaa tctacaataa tctttaattc atcataaatt tctttagaat
     8941 tcctgtgatt cttacatttt atttgctttg taaaatatgg ttgattatat attatatatc
     9001 cttgaggtgt agctattatt tcaaatcctc tagctcttat aagggctata tcttgtacta
     9061 ttacttgcct acttacattt aatatctcag ctagcattcc accttttaca gggcacttag
     9121 attcttttaa aatatctatt aatttgcttc ttctttcatc agaattcata ttacaccttc
     9181 tttctaaatc aaaaacttta cctatactat ataattctat attttatctt attataaatc
     9241 aatattgatt ttatattcta tttaatatta aactttattg caaataaaaa agattacgtg
     9301 gctacgtcct actctcccag gcagtttccc accaagtacc atcagcgcta gagagcttaa
     9361 cttctgtgtt cggaatggga acaggtgtat cctctctgct attgtaacca cataatcttt
     9421 atgcttaata tttctattaa gtttttagaa tctagtaata ctagtttctt tagagttaac
     9481 actctaaaaa ctacatatat attttattga atggacatca aataa
//
LOCUS       DAEPLF010000133         9419 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEPLF010000133 DAEPLF010000000
VERSION     DAEPLF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.133"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..1242
                     /locus_tag="KR314_002592"
     CDS             <1..1242
                     /locus_tag="KR314_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH0328963.1"
                     /translation="FVIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILR
                     NLTKIIKSNIFEEEMFARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKY
                     KLVLSCGIYFILPEDRDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEME
                     NTMFSSLENKEFIIYLQPKIDLNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGF
                     VINLDLYVLEEVCIYLRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPA
                     KYIELELTESIIFDNFDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKL
                     DQKFIMETYNSKRSKIIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYL
                     FGKPMPIEEFEHLIVSNLVRG"
     gene            1418..1786
                     /locus_tag="KR314_002593"
     CDS             1418..1786
                     /locus_tag="KR314_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0328964.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            1788..2513
                     /locus_tag="KR314_002594"
     CDS             1788..2513
                     /locus_tag="KR314_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0328965.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            2507..3271
                     /locus_tag="KR314_002595"
     CDS             2507..3271
                     /locus_tag="KR314_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0328966.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            3493..4743
                     /locus_tag="KR314_002596"
     CDS             3493..4743
                     /locus_tag="KR314_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0328967.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFIYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDCKAI"
     gene            4934..5818
                     /locus_tag="KR314_002597"
     CDS             4934..5818
                     /locus_tag="KR314_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBH0328968.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLVLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAVKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            6180..6797
                     /locus_tag="KR314_002598"
     CDS             6180..6797
                     /locus_tag="KR314_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBH0328969.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIITPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            6905..>9419
                     /locus_tag="KR314_002599"
     CDS             6905..>9419
                     /locus_tag="KR314_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0328970.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVKEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIM"
BASE COUNT         3676 a          856 c         1726 g         3161 t
ORIGIN      
        1 tttgtaattg atgctaacaa aatactatgt aaaaatactc aagtaaagta tgctttatta
       61 tatatagata ttgataaatt taaatatata aatgatttat ttggatatga agttgggaat
      121 gaaattttgc gtaatttaac aaaaattatt aagagtaata tatttgaaga ggaaatgttt
      181 gcaagaatat ctgcggataa ttttattata attatgaaat atatagaaga agaagatata
      241 acaaagaggt taaaaactat atttgaggaa ttagacttgt ttaataataa tcaagaagaa
      301 aagtataagt tagttttaag ttgtggaatc tattttatac ttccagaaga tagagatatc
      361 aactcaatca tagatagggc taatatacct cataaaatgg caaagggagg acataaaagt
      421 tcatatgcat tttatgataa taagattcat gaccaagaga taaaagaaaa agagatggaa
      481 aatacaatgt tttcaagttt agaaaataaa gagtttatta tatacttaca acctaagata
      541 gatcttaata caggagagat acaaggttca gaagcacttg ttaggtggaa acgtcctgat
      601 aaagggctaa tacctccaaa tgaatttatc ccattttttg aaagaaacgg ttttgttata
      661 aatttagatt tatatgtgct tgaagaagta tgtatatacc ttagaaaatg gattgatgca
      721 ggaataaatc cagttactgt ttcagttaat gtttcaagaa tacatttata ttgcaataat
      781 tttatagaaa catacaaaaa tataattgat aaatacaata tacctgcaaa gtatatagaa
      841 cttgaattaa cggagagtat catatttgat aattttgata ttttaattga tataatgaat
      901 aatttaaaaa aaataggatt tttaatatct atggatgatt ttggttcagg gtactcatca
      961 ttaaatatgt taaaagaaat tcctatggat atcttaaaac ttgaccaaaa atttattatg
     1021 gaaacttata atagtaaaag aagcaaaata attgttacta aagtaataga aatggcaaaa
     1081 gagttgggta tgaaagtcat atcagaagga gttgagacag aagaacagtt taaactttta
     1141 aaagaagtaa agtgtgatat ggcacaagga tatttgtttg gaaagccaat gccaattgaa
     1201 gaatttgaac atttaattgt atccaacttg gtaagagggt aattaatctg taatataaag
     1261 aatttaataa attgtgttaa aaatggactc taatgtagtg ttcatttttt ttgtgaaatt
     1321 ttttatactt ttactaaaaa gtaattgaca tatactttgt attattatat tattgtatta
     1381 ataaattaat acaataatat aataaggagg catgaacatg gaatgggaac ttgataataa
     1441 caaacctata tatatacaat tagtagaaca tttaaagtta aaaataattt caggggaaat
     1501 taaaatagga tctaaattag aaacagtgag agcactagca gaagatgcgg aagttaatcc
     1561 aaatacaatg caaaaggctc taacagaact tgaaagacaa ggtttagttt atagtcagag
     1621 aactaaagga agatttgtaa cagatgataa ggaaaaaata aaagctatga aagaggaaat
     1681 agctaatgtg gagataaata cattaaaagt aaccttagaa aagcttggat atgatagaga
     1741 tgaaatgcta aaacttatta cagaaaatct gaaaggagaa ttataaaatg gataaaaata
     1801 taagcaatgt ttctcaagat ggaatagttg aatttaaagg tgtaaacaag agttatggaa
     1861 ctaagaatgt tttaaaaaat attgatttaa atataccaaa gggaaaaata gtcgggctgt
     1921 tagggcctaa tggaagtggt aaaagtacaa tgattaagct aatgaatgga ttattacagc
     1981 ctgataatgg tgaaattatg ataaatggaa tgaaaccatc catagatact aaaaaaatag
     2041 tttcatattt accagaaaga acttatttaa atgattggat gaaggtatca gatttactta
     2101 agtttttcta tgatttttat agtgattttg atgttagaag agcaaatgag atgattaaaa
     2161 gcttagatat tgatgtaaat gaaaagctaa agacaatgtc taaaggaact aaagaaaaag
     2221 ttcaacttat tttagtaatg tcaagaaatg cgagcatata tatattggat gaacctatag
     2281 gtggtgttga cccagctgca aggtcatata tacttaagac tatattaaaa aattattctg
     2341 aagatagtac tttactgatt gctacccact taataagtga aatagaaaac atatgtgatg
     2401 agataatatt catatcaaaa ggagaaatag tgttacaagg agacgttgaa gcaataagag
     2461 aagaaaaagg aaaatctata gatgcattat ttagggagga atttaaatgt taggtaaatt
     2521 attaaaatat gagttaaaag caagtggaag aatatttata ccgttatata tagccatatt
     2581 gatagtagct gtatttaatg gaatatttat gaatacaaat atacttcagg tacagggaat
     2641 aggaatacta gtattaacaa gtctttttat ggcattagga gttttaacta ttgtagttac
     2701 aattcaaagg tttagaaaaa atcttttggg ggatgaaggt tatttaatgt ttactcttcc
     2761 tgttagcact agttcactta tattatctaa atgtataaca gcacttatat acgctgtact
     2821 tagttttata gttgcagtat tcacttttgg agttcttatg ctttttggta cttctggaat
     2881 tttactacct gaaattttag atttgtttaa cactagtttt aaatggattt ctgaaaactt
     2941 tttagatatt ttattattag tagtagtaat gtttatttca tattcatcat ttattttgtt
     3001 attatataca tcaatctcaa tgggacaatt accaaaattc aataaacata gaaacatagt
     3061 ggcatttgct tcttttatag ctataaatat agtaatatca attgttggag atgcagttgg
     3121 aagcatctta ccaaatgaag atacaaatat ggtatatcat ctttatcaga gtcctagttt
     3181 tatgcttgca atacttggta gtcttgtagt tgcaatagcg ttatttttcg ctacaaaatt
     3241 tatattagat aaaaaattaa atttagaata aatgttttta tatggagcca tcttttgagt
     3301 aatgttaaaa gatggctctt attatttatg tagttaattc atgtatataa tatgaatagt
     3361 attttatttc tagttaaata tatacatatg ttgtataatg atattgcgtg taaaaatttg
     3421 taagaataac tatacacttt atataagtta tatcaagaaa atttaaaaat atatggattt
     3481 gagggagaag atatgaaaag ttatacaaag gctaagtgga gtgtttgggg tataattaca
     3541 ttttcatttg ttttagtatt atttttaagg atgtcaactg ctgttgtttc agacaattta
     3601 gcaaatgagt taggttttaa ttctatacaa atttcaaata tagcatcctt ttgcctatat
     3661 gcatatgcat ttatgcaaat acctgcagga attttgattg ataaatatgg agcaagaaaa
     3721 attagcagtc ttggaataat aatggcaagt ttagggtcaa ttttatttgg tttgattcaa
     3781 agtatagaat tagcatatat ttctcgtgta attgtagggg caggaacatc agtaatatta
     3841 ctttgtattt taaaaattca agggagatgg tttaataagt cagaatttgc ttctgcaaca
     3901 gctaaatttt catttgtagg aaatttaggt ggagttcttg ctacttttcc acttgtgttt
     3961 ttgtctgaac ttgttggttg gagaaattca tttttattaa tcggtataat tggtgttgta
     4021 attggatgtt ttatatatat tattgttaga gatacaccaa aagagtatgg attcaatgta
     4081 gatactgaac cctatgaaaa gtcagagaag gttaatattg ttgatggtat taagtctgtt
     4141 ataaagaaca aatcaacatg gtataattca atgataatgt tctcttttgt tggtcttaca
     4201 agtgcattta taagcttgtg gggagtaaga tacataatgg acgtttatgg tgtgagtaag
     4261 agtttttctg cgtttatagt atcatttttt acatatggat ttatatttgg ttcaataatt
     4321 atggattttg tatttgctaa aataaggtct agcaaattta atataataaa atttggagca
     4381 atgatagatt tgtttatatg gattgtaatt gtagttgtat accaggtaaa accaccaatt
     4441 atagttttac caatatcatt ctttattatg ggatgtatag ttatgtcaca tctacaggtt
     4501 tttaatgatg ccaagtataa aaataaagaa atttattctg gactggcaac tagtgttatt
     4561 aatacttttg aatttatagg aagtggaatt attaatttga ttatagctat ctctttacaa
     4621 gtaaattcat ataatatagt tgatggatat aaaaaaggtt ttgtagtatt tatagtacta
     4681 agtattataa ctatagtatc atctcatata ggggtaaaaa atgatgattg taaggctata
     4741 tagtgtttta ttaatagttt aatttagtat tatattttgc atatatataa caaatagttt
     4801 gcattttcct aagatgtata attggaatat aaatcttgtt tactatgtac aaatggtatt
     4861 gtattatatt aaatagaaaa taagaacttt aaactataat attggttcta aaatataagg
     4921 taaggagttt tttatggata cgacgaaaat agatgatata agcaagttag atttaaataa
     4981 tttagacatt aataagatgg atatgggagg tataatggaa agtctgctag aatgggctac
     5041 tacaagcgga gtaaaactta taataggtat acttatactt tctattggat ttaaaataat
     5101 aaaaaaattt gtaaaccatg ttatgttagt acttagtaaa agagatattg atttaacttt
     5161 aagaaggttt ttgaagtctc ttcttttgag tgtacttaaa gttgccgtta taatcattgt
     5221 tctggaatat tggggaatga gtttgtccag ttttgctgct gtgatagcat cagcaggggt
     5281 agcaataggt ttggctttac aaggtagtct atcaaatttt gctggaggat ttattatact
     5341 attgattaga ccttttaaag taggagatta cgtagaagca gcaggccatg gaggaacggt
     5401 tgaacaaata ggactttttt atacacaatt ggttactcca gataataaac agatacttat
     5461 acctaatggt tctgtatcaa atgatagtct aataaactat tcagcaaaga atactagacg
     5521 agttgatttg acttttagtg tagggtatga agatgatata ctacatgtta gaagagtact
     5581 taaagatatt gttaataatc ataaattaat aataaatgag ccagaaccat ttataggagt
     5641 agtagaacat ggagataatg ctgttaaatt tgcaactaga gtttggtgca agacagaaga
     5701 ttattggaca atttatcttg atttactaga agaggttaaa gttaggtttg acgaagaagg
     5761 aattacgatt ccatatccta aaatggattt aacagtaaaa gaattaaaca aaatttaatt
     5821 ttttaatata gtgtttaaat ttaagaatat aatatagata tacttataag gtttgtaaaa
     5881 aattaataag tactgttatt ttgtaagtaa aaatataaaa gaatgggagt gtctcaaaat
     5941 gaacttttta gttcatgagg cactcttttt gtacgaaata aaatatattt ttattatttc
     6001 aacaatgaaa aagatgcttg tttctatttt gagacagtat aatttttatg taactttaat
     6061 tttagtaaat ataaagtttt aaaattgata aaataggagg aatttttaat ataaaagggt
     6121 aaataaaggg tttagagggc ataatataga ataaattaat atatttaagg aggattttta
     6181 tgtgtaaaaa aacgtattta ttagtggtaa gtatacttat agcaggttta ttaactgcat
     6241 gtagtgggag cccaactaag gattcaggac aagcacaaga taatacaaaa aaggaggcaa
     6301 atgcttcaaa taatgcttta aaagatgaaa aaaataatga aaatttaatg gaacaagatt
     6361 ttaaagtgcc atacacagat gcaataaaca tatttaaaga taaatacaag gatgctgata
     6421 tagtagattt aagtcttgag agagatttaa ataaatttgt ttatactgtg gaaggtgtag
     6481 atgataataa tgaatataag atgaaaattg atgctaatac aaaagatgta ttagaagata
     6541 aaacagaaaa gttagattca gaagatttaa atggagttgc tagaaaagaa aaattagatt
     6601 taaatgacat aattacacct caacaagcta tggaaattgc attaaaagaa caaaatggaa
     6661 tggttaaaga atggagttta gacaaagatt tagatgttac tttttataaa ataagaatag
     6721 ataaagataa aaatgaatat gacataaaag tagattctaa aaagggaact gtattgaagg
     6781 tagaaaaaga agattagaag atttaaacta tctttaaaat aatttctttt ttgaataaat
     6841 tttgtttaaa atataacaag cagggtataa agagactaaa taactatttt aggaggagta
     6901 cggtatgtca ttgaaatcac taaaaataaa agaaaattta tattgggtag gttcacttga
     6961 ccctgattta agagtatttg acataattat gtatacacct tatggaacta cttataattc
     7021 atatgtatta aaaggaacag aaaaaacagt tttgtttgag acagttaaag ataagcattt
     7081 tgacaattat attgaaagat taaatgattt aaatatagat tttgaaaaaa tagactatat
     7141 tgttgtaagt catactgaac cagaccatgc aggaagcgtt gaaaagcttt tggatttagc
     7201 taaaaatgca aaagtagtag cttcagaaac agcaattaaa tatcttaagg aaatcgtaaa
     7261 caaagacttt gaatatgttg cagttacaga tggagataca ttatcaattg gggacaaaac
     7321 tttagaattt ttctctgtac ctatgcttca ttggccagat actatataca catatataaa
     7381 agaggataaa actcttgtaa catgtgattc atttggtagt cactatagta atgataagat
     7441 agtaaataca cttgatgaaa atgaagaaaa ggattacttg gatgctttaa gatactatta
     7501 tgattgtata atgggtccat ttaaaccatc tatggtaact gctatagaaa agataaaaga
     7561 tttagatata gatactgtat gtccaggtca tggtccagta ttaactgaaa atcctagaaa
     7621 aattatagac ctttattata actggagtgt aaaggaacaa ataaagttag aaaaagaagt
     7681 tacaatttgt tatgtttctg cacatggata tacaaagatt atggcagaag ctataaaagc
     7741 atatattgaa aaaaatagca actataaagt aaatttattt gatgtaatag agcataagca
     7801 agaagatatt ttggcaaaaa tagcagtatc acaaggtgta ttatttggaa caccaacaat
     7861 attaggagat gcgttaaagc ctatatggga tatattaata tcgttaaacc cagttcttca
     7921 tggtggaaaa gttgcttcag tatttggttc atatggatgg agtggagaag gtatagaaaa
     7981 tgctatggag agaataagtc agcttagaat gacagcagta aaaccttttg ctgttaactt
     8041 taaaccatca aatgaagaga ttgataaatt acattcttat acaggtaaat tcctagataa
     8101 gttaaattct acttttggaa gtaaaaagaa aactaagaaa tttaaatgtg taatttgtaa
     8161 tgaagttttt gaaggtgata gtgctcctag tgtatgccct gtatgtggag ctaaggaaga
     8221 ccaatttata gaagttgaag aagatgaagt tacttttaga aaagatactg atgaatattt
     8281 cgtaatagta ggaaatggag cagctggttt ttatgcagca gatgctataa gaaaaagaaa
     8341 taaaacttgt aagataacta tgatttctaa tgaagatgag ttgacttact atagacctgc
     8401 actttctgat ggtataaatg aagaacttgg ttcagatttc tacatggaag ataaagattg
     8461 gtatgataaa aataatatag tagtaatatt aggaacgaat gtagataaat tagatgaagt
     8521 gaataaaact attatagtga atgatggagc tattaaattt gataagcttg taatagctac
     8581 aggaagtaga aactttatac caccgattaa gggccatgat ttagaaaatg tgtttactct
     8641 tagaaatata aaagatttat atagtgtaaa agaagcacta gaaaaatcta agaaagttgt
     8701 agtgattggt ggcggtttat tgggacttga agctgcatgg gaatttagac taaaaggatt
     8761 agaagtagta gtagttgaag ccatggatag catactatca aaacaacttg ataaagaggg
     8821 tagtaaaata ttagaacaat gtgtaagaga tacaggaata gatgtaagat tgggtgtagc
     8881 agttgatggt attgaaggtg atggaaaagc tcaaaaagtt gtatttaaag atggagatag
     8941 tgttgactgt gatatggtag tattttcaat tggtgtaaga gcaaacactc aaatggttca
     9001 ggatacatct gtaaaaattg atagaggtat tgttgtagat aagacattac agacaaatgt
     9061 taaagatata tatgcttgtg gagatgttgc acaagtggga aatattagtt tagcaatatg
     9121 gccatcttca gtagaaatgg gaaaaatagc aggagcaaat gcaagtggag ataatttgac
     9181 atttgaaagt gaagtatatc cagtttcatt agatgcaatg aatgtaaaag tatttagtat
     9241 tggtaatata caaaattttg ataaagaaat ttcatcaaaa gatgaaggtc aaagaatata
     9301 taaaaaatta tttatgaaag atggttcatt agttggagct atacttataa atgatttaag
     9361 ctgtactgtt aaattgataa gattaatatc tgaaaaaggt gattttgagg atattatga
//
LOCUS       DAEPLF010000134         9299 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEPLF010000134 DAEPLF010000000
VERSION     DAEPLF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.134"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            279..1682
                     /locus_tag="KR314_002600"
     CDS             279..1682
                     /locus_tag="KR314_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328971.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            1709..3253
                     /locus_tag="KR314_002601"
     CDS             1709..3253
                     /locus_tag="KR314_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBH0328972.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            3284..4675
                     /locus_tag="KR314_002602"
     CDS             3284..4675
                     /locus_tag="KR314_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBH0328973.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            4868..6364
                     /gene="abfD"
                     /locus_tag="KR314_002603"
     CDS             4868..6364
                     /gene="abfD"
                     /locus_tag="KR314_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBH0328974.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            6499..6774
                     /locus_tag="KR314_002604"
     CDS             6499..6774
                     /locus_tag="KR314_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0328975.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            6798..8105
                     /locus_tag="KR314_002605"
     CDS             6798..8105
                     /locus_tag="KR314_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBH0328976.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            8125..9243
                     /locus_tag="KR314_002606"
     CDS             8125..9243
                     /locus_tag="KR314_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBH0328977.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
BASE COUNT         3558 a         1051 c         1901 g         2789 t
ORIGIN      
        1 gaatagtgct attattaaat taacaaaaga gaatagctgg tatggtttag agtaaatcaa
       61 tctatattac agttatatca acgaatatca aaaaattatc tattagaaaa taaaaattat
      121 ttttacaatt aaattgttaa ttaaatcata aagaaaaaag tgatattaac aagataaaat
      181 ttttttaatc agaaatgtta atttaataac aaaatagagt attggggaga aaggagaaat
      241 atatttttta ataatatttg ttaatttaat aacgaaaaat gaaagggagt gatttagtga
      301 agtttgaatt gatggatttt ttaatgaatc cttttgtcct tatgtttgct gcagtaatta
      361 caggaatttt atttggaaaa attaaatttg ggaagtttaa ttttggggta tcaggagcat
      421 tatttacagg attatttatt ggatggcttg catatagctt ggggaatcta ataatagcaa
      481 aaggagaaac agcagcagga tataaggcag caactgtaat gatggggaat ggaattattt
      541 cttctgactt ctttgatttc tttttaataa tatttgttgc agcagttggt ctattggcag
      601 caaaagatat gaaagctgta ttaaagaagt atggagcaag atttgtaatt ttaggagttc
      661 ttataacttt tataggtggc tctatgactt atgcaatgac tttactatca agtgataaag
      721 gaagtagtgc ttatgaagtt tctggcgttt acacaggagc attaacaagt tcgcctggat
      781 tagctgctgc tttagaaaca gctggtaaac atgcagaaga tgtatcaaaa gaatttgaaa
      841 aagcttcaat aaaagataaa aaagaaattt taaaagtagt cgacccagaa ggcaaattgg
      901 atgtgaatac aactacatca ctgactcaag agcaaataga taagtatatt gcatatgctg
      961 aagctggtgt tggtataggt catgcagtcg catatccttt tggagtttta atagtaatct
     1021 taggtgtaaa tttccttcca aaattattta ggatggacct aaaagaagaa agaagaaaat
     1081 atgagaagga aatgaaggaa gcaagagata gtgtttctgg taaaacgata ccagaagttc
     1141 catttaatat aataacattt ttcttgacat gtttagctgg atacttagtt ggaggaatac
     1201 atgtatttat gggaccacta gggtacttca cactaggcgc aacaggaggt tcactaattg
     1261 tatctctagt tcttggatat ataggtaaga taggggtagt aaatttcaga atggaagaaa
     1321 aggttttaaa tattttaaaa caaatagggc tagtattttt cttagcaata gttggattaa
     1381 gatatggtgg aaaagtagtt gattcaatta tgacatctgg tatgcaccta gccttagtag
     1441 caatagcagt aggtgttaca gctatgatga taggattctt agttggcaaa tacgtattta
     1501 aattaaattg gattttatta tctggagctg tctgtggtgg tatgacatca acaccaggtc
     1561 ttggagctgc tgttgatgct cttgatagtg atgacccagc agcaggttat ggtgctacgt
     1621 atccatttgc attattaact aaagttatat tggttatagt tttacacaaa cttccaatgt
     1681 aaataagatt ttatatgggg ggatatttat gaataatgaa ggagcatttg aagaattaag
     1741 tggaagatta agaaataaag aattgtgtga tttaataatg aatgcagatg aagctgctaa
     1801 aattatagag gatggcatgg ttattggagt tagtggattt acaccttcag gctatccaaa
     1861 agcagtacca ttagcagttt cagaaagagc taaaagtgga gaaaagataa aattaaccgt
     1921 atattcagga gcatctttag gcccagaagt tgatggagca tggtcagaag caggtataat
     1981 tgaaagaaga cttccttatc aaactaattc aatacttaga aataatatta ataaaggtgt
     2041 tgttgattat atagatatgc acctaagtca ttctactcaa tttttaaatt atggaactat
     2101 acctaaagta gatgtagcta tagtagaagc acttgcaata actgaagaag ggaatattat
     2161 accaactagt gggataggta attctccatc ttttataaaa agtgcagata aagtaatagt
     2221 agagataaat cttgcaaaac caatggaaat ggaaggtatg gcagatatat atattacgga
     2281 aaatccacca aatagaaaac caatagaaat aaatcatcct aaggatagaa ttggaacaac
     2341 atatatacct tgtggtttgg ataaaattgc aggagtagta attaccaata tgcaagataa
     2401 gacccgtcca ttgggggttg tagatgaagc ttcgaaaaaa atatcaaata atattattgc
     2461 ttttttaaga gaagaagtaa aggcaggcag attatcaaag aatttacttc ctcttcaatc
     2521 tggtgtagga agtgtagcaa atgcagtatt atatggatta tgtgaatctg aatttgaaaa
     2581 tttaacttgt tatacagagg ttgtacagga ctctatgttg gatttaataa gaatgggtaa
     2641 agttacaatg gcatctacta catctgtaag tccatctcca gaaggattaa taaaatttga
     2701 aaaagatatt gattttttta aggataagat tatattaaga cctcaagaaa ttagtaacaa
     2761 tccagaaata gccagaagaa ttggtgtaat tgcaatgaac actgcaattg aagttgatat
     2821 ttatggaaat gtaaattcta ctcacataat ggggtcaaag atgatgaatg gaattggagg
     2881 ttctggtgat tttgctagaa atggagcaat aactatattt agtactgagt cgattgcaaa
     2941 aaatggtgat atatcatcaa tagtacctat ggtatctcac gtagaccaca ctgaacatga
     3001 tgtaatggta attgtaacag aacaaggata tgctgattta agaggtcttg cacctagaga
     3061 aagagctata aaaataatag aaaattgtgc ccatccagat tataaagaac aacttaggga
     3121 ttatttaaat agagcttgta aaagtggagc aaaacaaaca cctcacatat tggacgaagc
     3181 tttatcatgg catagtaaat ttatgagtac aggtacaatg aaaaaggctg aaacttttaa
     3241 aagtgcatta taaaatattt aataaattgg aggtagattt taaatggaaa aagcagtgga
     3301 aaactttgaa gatttaagta aggaatacat taatggttat attgagagag caagaaaagc
     3361 tcaaagagag tttgaatgtt atactcaaga acaagtagat aaaatagtaa aaatagttgg
     3421 aaaagtagtg tattataatg ctgaatattt agctaaactt gctgtagaag agactggaat
     3481 gggagtatat gaagataagg tagctaaaaa taaaagcaaa gcaaaagtta tatataacaa
     3541 tttaaaagat aaaaaatcag ttggtataat tgatatagat agagaaactg gaataacaaa
     3601 ggtagctaaa ccagttggag tagtagcggc aataactcct tgtacaaatc caatcgttac
     3661 acctatgagc aatgcaatgt ttgcacttaa gggaagaaat gcaattatta tcacacctca
     3721 tcataaagca attggatgta gtacaaaaac tgtagagatg attaatgaag aattagaaaa
     3781 aataggagct ccagaaaatt taatacaaat acttgaccaa caatcaagag aaaatacaag
     3841 aaatttaatt tcatcagcag atgtagtaat tgccactgga ggaatgggaa tggtaaaagc
     3901 tgcgtactca agtggaaaac cagcacttgg tgtaggggca ggaaatgttc aatgtatcat
     3961 agatagagat gtagacatta aagaagcagt acctaaaatc atagcaggaa gaatttttga
     4021 taatggtatc atttgttcag gtgaacagtc agtaatagta gcagaagaaa tgtttgataa
     4081 aataatggat gaatttaaaa ataacaaagg ttttatagtt agagataaag tacaaaaaga
     4141 agctttcaga aatgctatgt ttgtaaataa atctatgaat aaagatgcag taggtcaatc
     4201 agttcataca attgcaaaaa ttgcaggtgt tgagatacca gaagatacaa aaataatagt
     4261 aatagaagct gatggaccag gagaagagga tattattgca aaagaaaaaa tgtgtccagt
     4321 tatatctgca tacaagtata aaagttttga agaaggtgta gctattgcaa aagcaaattt
     4381 aaatgtagaa ggaaaaggac atagtgtatc tatacattca aatacagtta aaaatataga
     4441 atatgcagga gaaaatatag aagtatcaag atttgttatt aatcaatgtt gtgctactag
     4501 tgctggagga agtttcttta atggtcttgc acctactaat actctaggtt gtggttcttg
     4561 gggaaataac agcatctctg aaaatcttga ttataagcat ctaattaata tttctagaat
     4621 tgcttattat atgccagaaa atgaagtccc tacagatgaa gaactttggg gataaaactt
     4681 agacaaaaaa tattcattct aaattaaagt aggaatatat tttaatttag aatgaattta
     4741 agacataaat taaatttaaa ttagtttatt ggactaaatt taaaatatta agagacatat
     4801 agaagaagga agtattataa tttaaaataa ataaaaaaat ctataaaaga gaaggagtgg
     4861 ttttattatg gcattaatga caggagctca atatattgaa agtttgagaa aattaaatac
     4921 taaggtttat atgtttggtg aagaggttaa gaattgggtt gaccatccta tgattagacc
     4981 atctataaat tgtgtagcag caacttatga cttagctcat gacccagaat atgcagattt
     5041 aatgacagtt acttcaaata tcacaggtga aaaaataaat agatttggtc atcttcatca
     5101 aagtgttgat gatttgataa aaaaagtaaa aatgcaaaga ctatgtggtc aaaaaacagc
     5161 ttcttgtttc caaagatgtg ttggtatgga tgcgtttaat gcagtgtatt caacaacatt
     5221 tgagtgtgat aaagcccatg gaactaatta tcatgataat tttgtaaaat acttaactta
     5281 tatacaagaa aacgatttag tagttgatgg agctatgact gaccctaagg gagatagaag
     5341 tctatcacct agtgctcaac ctgacccaga tatgttcctt catatagtag aaagaagaga
     5401 agatgggata attgtaagag gagcaaaggc acatcaaact ggttcaataa attcacatga
     5461 gcaccttata atgcctacta taagtatgac tgaagcagat aaagactacg ctgtatcatt
     5521 tgcagttcct tcagatgctg aaggtgtgtt tatgatttat ggaagacaat cttgtgacac
     5581 cagaaaatta gaagaaggtg cagatgttga tttaggaaat aaggagtttg gaggtcaaga
     5641 agctttagta gtatttgata atgtatttgt tccaaatgat agaattttct tatgtggaga
     5701 atgggatttc tctggtatgt tagtagagag atttgcagga taccatagac aaagttatgg
     5761 tggatgtaaa gtaggagtag gtgatgtaat aataggagca gcagctttag cagctgatta
     5821 taatggagca aacaaagctt cacatataaa agataaacta atagaaatga ctcaccttaa
     5881 tgaaagctta tattgctgtg gtatagcatg ttcatcagaa ggtcacaaaa cagaagctgg
     5941 aaactatcaa atagatttac ttcttgcaaa tgtgtgtaaa caaaatgtaa ctagatttcc
     6001 ttatgaaata gtaagacttg cagaagatat agctggaggg cttatggtta ctatgccttc
     6061 agaaaaagac tttaaatcag accttaaagt tggaacaagt ggaatgacta ttggcgaagt
     6121 atgtaacaag tattttaaag cttcttcagt ggcttcaaca gaagaaagaa tgagaatatt
     6181 aagattctta gaaaatatat gtcttggttc atctgctgtt ggttatagaa ctgagtcaat
     6241 gcatggggca ggttcaccac aagctcaaag aataatgatt tcaagacaag gaaacataaa
     6301 tcaaaagaaa gaattagcta agaaaattgc gggaataaaa aaagaggaag ctttaaattt
     6361 ataaaaagtt gatatataaa gggaaattat atattaatgt gtattaatta atgaaaagat
     6421 atgtagtata agagatagtt ataaaattat ggtggttttt agctaagtaa agaaattttt
     6481 aataaggaga atttacacat gacctgtgga gttagatttt gtgggggctg taaccctaga
     6541 tacgaaagag ggaaggctct tacaataatc aaagagcact tcaatggtag ggtagatttt
     6601 agcatagcac aagagaacgt cgagtatgat ttgctactta taattggagg atgcgcaact
     6661 ctatgcgcat cctatcaaca atataaagtg aaaaatgaat gtgttaagat gtgggatttt
     6721 agccatatag aaagagttat aaatgtgatt gaatcagcaa tttgtgaaac ttaaaaaagc
     6781 taaatggggg gatttcattg agttggcaag aattgtatca aagtaaatta tgtagtgcta
     6841 cagaagctgt aaaacagatt aaaaacggtg atactgtagt atttgcacat tgtgtaggtg
     6901 aacctcctgc actagtagag gctatgattg aaaatgctga acaatataaa gatgttgaga
     6961 ttaaacatat ggtaagcttg ggtagtggtg gatatactgc taagggaatg gaagctcatt
     7021 ttagagtaaa tccaatgttt gtaagtggaa atgtaagaaa ggcaattgaa aatggagatg
     7081 gtgattttac acctgcattc ttccatgaag taccaaagtt gcttagagaa aaaagattaa
     7141 aatgtgatgt tgttttagca caggtaactc caccagatga acatgggtat tgtagcttag
     7201 gaacatcagt tgattatact tatgaagcca ttaaaactgc tagaacagta attgttcaag
     7261 tgaatgacca gtttcctaga acctatggtg aggtagtcca tgtatcagag tttgactata
     7321 tagtggaaaa atcacaacct ttatttgaac tacaacctgc aaagattgga gaagtagaag
     7381 aagcaattgg taaaaattgt gcttcattaa ttgaagatgg ttctacttta cagctaggta
     7441 ttggtgggat accagatgca gttatgttat ttttaactga taaaaaggat ttagggattc
     7501 attcagaaat gatttcagat gggactttgg ccctttatga aaaaggtgtt ataaatggta
     7561 aatataaaaa ttttgataaa gaaaaaatga cagttacctt cttaatgggt actaaaaaat
     7621 tatatgactt tgcaaataat aaccctgcag tagaggtaaa accagtagac tatgtgaatc
     7681 atccagcaat tataatgaaa caacataaga tggtttctat aaattctgct atacaagttg
     7741 atttaatggg acaggtagtt gcagaggcta tgggacttag acaatttagt ggtgttggag
     7801 gtcaagttga ctttatacgt ggagtgtcta tgggggaaga tggaaaggca attatagcta
     7861 tgccttcaat aactacaaaa aaagatggta cagtaatttc aaaaatcgta tctattgtgg
     7921 atgaaggtgc acctataacg acatcaagaa atgatgttga ctatattgta acagaatacg
     7981 gtatagcaga attaaaagga aaatctctta gagaaagagc aagaaattta ataaatatag
     8041 ctcatccaag tgtaagagaa agtctagcag tagaatttga aaagagattt aaggagaaat
     8101 attaataata gaggggtgtt aataatggaa gcacttagag tagttccaaa gatattttat
     8161 tttgatacat ttaaagaatt taatgaagaa tttaaaatag gaaaaaatga tttagtaatt
     8221 actaatgagt ttatatatga gccatatatg aaacctcttg gaatagatac aaatttaatt
     8281 tttcaagaaa agtttggaac aggtgaacca tctgatgaaa tgatagactc catgacaaag
     8341 gaaatgaaga aatacaattt tgatagaatt attgcgtttg gaggaggaac aatagttgat
     8401 atatgtaaga tattagcact tgatgttcca gaaaagtcta ttgatttatt tgaaggagaa
     8461 gtatcaccta aaaaagtaaa agagttagta gtagttccta caacatgtgg tacaggtagt
     8521 gaagtaacaa atgtagctat tgcagagtta aaatctaaac atactaaaaa ggggcttgca
     8581 gtagaagaaa catatgcaga ctatgcagtg ttaattccag agacagtaaa agggttacct
     8641 tacaaattct ttgttacaag ttcggtagat gctcttatac atgctattga gtcttatctt
     8701 tctccaaaag caagtccatt tacagagatg tattctctac aagcaataaa aatgattatg
     8761 gatgggtata agaagatagt tgataaagga gaagaagaga gatttaatca tcttagagac
     8821 tttgtacttg catcaaatta tgcaggaatt gcttttggaa atgctggttg tgcagcagtt
     8881 catgcactat cttattcaat aggtggagct ttccatgttg cacatggtga agcaaattat
     8941 cagttcttta ctgaagtatt taagatgtat tctagaaaaa aacctaatgg aaaaattata
     9001 aaatgtacaa aaattttagc agatgccctt gaatgtgacc ctaactgtga tgtttatgga
     9061 gaattagaaa agtttttaaa taaattaata gctaaaaaag ctttgagaga atatggaatg
     9121 gtggaaagtc agatagatga atttacagat agtacaatag caaatcaaca acgactactt
     9181 gctaataatt atgtagagct tagtagagag gaaataagag agatatttgc aaatctttat
     9241 taatacaatt ttttatttat atgtatagtc aacataaaca atgctaatta tatttatgt
//
LOCUS       DAEPLF010000135         9255 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEPLF010000135 DAEPLF010000000
VERSION     DAEPLF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9255
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.135"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            123..1313
                     /locus_tag="KR314_002607"
     CDS             123..1313
                     /locus_tag="KR314_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0328978.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDIVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLVRKGFKH
                     TPLQSLCPEYKDKIITCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    1447..1702
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1761..2762
                     /locus_tag="KR314_002608"
     CDS             1761..2762
                     /locus_tag="KR314_002608"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBH0328979.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            2866..3255
                     /locus_tag="KR314_002609"
     CDS             2866..3255
                     /locus_tag="KR314_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBH0328980.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIIKKDKHSNIIENVEFSIKDEEE"
     gene            3416..4624
                     /locus_tag="KR314_002610"
     CDS             3416..4624
                     /locus_tag="KR314_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBH0328981.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(4711..5787)
                     /locus_tag="KR314_002611"
     CDS             complement(4711..5787)
                     /locus_tag="KR314_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0328982.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            6016..6543
                     /gene="hpt"
                     /locus_tag="KR314_002612"
     CDS             6016..6543
                     /gene="hpt"
                     /locus_tag="KR314_002612"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBH0328983.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            6632..7564
                     /locus_tag="KR314_002613"
     CDS             6632..7564
                     /locus_tag="KR314_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBH0328984.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIVGWMLGIFFTVIF
                     IKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            7626..8852
                     /locus_tag="KR314_002614"
     CDS             7626..8852
                     /locus_tag="KR314_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0328985.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTRFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINANTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            8936..9157
                     /locus_tag="KR314_002615"
     CDS             8936..9157
                     /locus_tag="KR314_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBH0328986.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
BASE COUNT         3618 a          960 c         1632 g         3045 t
ORIGIN      
        1 attttaaatc tattggacaa aaaagtgata ttattataat attaagaatt gtaaaataat
       61 accaaagtgc atctagtaat ggacttaaca attataaaaa ttttattctt aggagggaaa
      121 aaatgagtaa gtataatttt gacagagtaa tagatagagt aggaacagat tgtgtaaaat
      181 gggattttag aactaattgt tcaacaaaag cacaaaaaga tggtttgcct ttttggattg
      241 ctgatatgga ttttgaatgt gcagaaccaa taatagaagc attacgtaaa aaagttgaac
      301 ataaaatatt tggatatagt tcaaatgata gtgataaata ctttaatgca gtttgtgatt
      361 ggtataaaag gagatttaac tgggaaataa atagaaaaga tattattttt agtcctggtg
      421 ttgtacctgc tgttgctatt ttagtaagaa tattaactaa ttctaatgaa ggtgtaatag
      481 tccaaaaacc agtgtattac ccatttgaag ctaagataaa gagcaataat agggacattg
      541 taaacaatcc tctaatatat gagaatggta cttataggat ggattatgat gatttagaag
      601 aaaaagctaa gtgtagcaac aataaagtac tgatactttg tagtcctcac aaccctgttg
      661 gaagagtttg gagagaagat gaattaaaga aggttgtaga aatatgtaaa aaatatgacc
      721 tatggataat agctgatgag atacattctg atttagttag aaaaggattt aaacatactc
      781 ctttacagtc attatgccca gagtacaagg ataaaataat tacatgtata gctccaagta
      841 aaacatttaa tctggcagga atgcagttgt ctaatataat agtgaataat gatgaactaa
      901 aaaagaaata tcaagaagag gttacagctg ttggagttgc aacttcacca aatccctttg
      961 ctattgtagc tacaatagca gcatataatg aaagtgaaga ctggcttaat gaattaaatg
     1021 attacttaga taataatata caatttattg atgaatactt aaaagaaaac ctaccaaaag
     1081 taaaacttgt ttatccagaa ggaacatatc tagcatggtt agattttact gcttatggat
     1141 taaatgaagt agaacttgag gatttaatgt ttaaaaaagc taatgtatta tttgatgaag
     1201 gttatatatt tggaaaagaa ggaataggat ttgaaagaat aaatgtagct tgtccacagt
     1261 ctttattaaa agaatgtatg gacagattaa agactatatt tgaaaattta taaagtatag
     1321 gtaaaaattt ataaagtgtt taaaaataaa aaaataataa acttgacaaa ttttaactaa
     1381 tcacttatca tactattatg aataaataga aaacataaga gagctaaatt ttattgaatt
     1441 aaactcttaa agactttgaa aggaagagta gttatagaat gtaattaaag cgagttggtg
     1501 atggtgtgag accaatatga agtctataat gaagaacatc ctggagttgc taaccgaaat
     1561 tttaaaagag taggcttagt cggaaccaaa ccgttatcat attggggtgt atcgagtaat
     1621 atctgtacat tgtgagctgg gtttgaattt atttcaaact aatcagggtg gtaccgcgga
     1681 aaagcaacct ttcgtccctt ttatataggg atgaagggtt tttttaatgg aaaaatatat
     1741 aaatataggg agtgaatgat atgagcacaa taataccaaa ggattataaa agtagtttga
     1801 acgtaattga tacacaaata gctataaaaa aattaaaaga tttttttgaa atgagactaa
     1861 gtaatgaatt aaatcttata agagtttctt ctccgttatt tgtattgcca gaaacaggtg
     1921 caaatgataa tttaaatgga gaaaaggctg taagctttga tataccattt atgaataaaa
     1981 atgcagaaat tgttcaatca cttgcaaaat ggaaaagatt agcccttaaa aaatatgggt
     2041 ttgaagttgg tagtggttta tatacagata tgaatgcaat aagaaaaaat gaagaattag
     2101 acaatataca ttctctttat gtagaccaat gggattggga attggttata gataaaagta
     2161 gtagaaatga aaagactcta aaagatgttg taaaaagatt gtatggagta tttaaagata
     2221 ctgaaatttt tgtatgtagt atgtatgaag gtataaaaga aatattacca gaagaaataa
     2281 cttttataac atcacaagag ttagaagata tgtatccaga attatcaccg aaggaaagag
     2341 aagataaaat tgtaaaagag aagaaagcag tatttataac tcaaatagga aaaacattaa
     2401 tatcaggaga aaaacatgat ggaagaagcc ctgattatga tgattgggaa ttgaatgggg
     2461 atatattatt ttacaatcct gttttagatt cagcattaga attatcttca atgggaatta
     2521 gagttgatga agaatctctt gatagacaat tgaaacttgc tggatgtgaa gagagaaaag
     2581 agtttgacta tcataagatg cttttaaatg gcgaacttcc gtatacaatt ggtggaggaa
     2641 ttggtcagtc aagaatatgt atgtatttct tacaaaaagc tcatatagga gaagtacaag
     2701 ttggagtatg gccacaggat atgattcaaa attgtagtag tgcaggaatt gaattattat
     2761 aatttaaata taaactaatt gataaaattt atatctaata tgttcatcaa attaattata
     2821 tcggttataa tgtagaataa cctataatta taagaggtga taaatatgga gtttttaaaa
     2881 ataatcatta atatagtttt agatatttta aaaaaaattt tggtcagatt taaaaatgct
     2941 aaatttggtt tggtttttgt atttgattta cttaaactac ctgattttat gacagataaa
     3001 agaatcaata tagttgataa aataaaggta atttctgtat taatttttac tatatcctac
     3061 tttgtatctg gggttgatat aattccagag atgattgcag gggcatttgg ttttatagat
     3121 gatgctatag ttttaatttg gagtataggt atagtgaatg aagaaattaa taagtataga
     3181 gttattataa aaaaagataa acattcaaat ataattgaaa atgtagaatt ttctataaaa
     3241 gatgaagaag aataacaatt tatatatgtt attctttttt ttattaacaa aaaatggtta
     3301 aattgttgac ggagaggtaa aactctatta tcataataag tggatatcca atatttaact
     3361 aagtattcaa tgtgcaagaa tgcttaattt aaaaaagtta tggagggtaa atattatgaa
     3421 gaaaaaaata ggtttagtgc ccaaactaat catcggtatc atagtcggta ttttaatcgg
     3481 aagttatgca ccagaaataa tagtacaaat attagtaaca gttagtacct tattctcagc
     3541 attcctaaaa tttgttatac catttataat tataggtttt gtaacagcag gtatagctga
     3601 tttagcaaca ggtgcaggaa agttattggg gataactacg ggaatagcat atggttctac
     3661 cttaatagca ggattattat catttgcagt atctacatta atcttcccaa attttattga
     3721 tgcaagtgtt gcaagtcaaa ttggagaccc agaggcagga atgttagctc caatattcac
     3781 aattccatta gagcctatgg tagatgtaac agctgcaatc gtatttgcat ttgtaatggg
     3841 acttggaata tcagctttaa gaaatcatgg aaaaggagag actttattta atttcttcca
     3901 agaatttcaa ggaatcgtaa cgaaaacgtt gtcaacagtg attatacctt tattaccttt
     3961 atacatagca ggaacttttg caaatataac tatagcaggg gaagtatgga caatattagg
     4021 ggtattctgg aaagtatact tagtagtaat acctttacat tttgtatata tggcatgtca
     4081 attcacagca gcaggtgtat tttcaggaaa aaatccagta agaatgttaa aaaatcaagt
     4141 acctggatat ttaacagcta taggaactca atcttcagca gcaactatac cagttaatgt
     4201 tggttgtgca gaaaaaaatg gagtaagtaa gcaaataaga gaatttgttg tacctctttg
     4261 tgcaacaata catttatcag gtagtatgat atcaataaca ggatttacaa ctgcagtttt
     4321 aatgatgaat ggtatgccac atggaatagg tgtattattc ccttatattg caatgttagg
     4381 tatagcaatg gttgcagcac caggagcacc aggtggggct ataatgagtg cacttccttt
     4441 cctaccaatg gttggaatac catcagatgg tgggcttgca agtttaatga tagctttata
     4501 cttaactcaa gacagttttg gtacagcatg taatgtatca ggagataatg caatagcagt
     4561 agtagtagat aaaataaatg atggaatgag taaaaaagga aacaaagaag agcaaagtgc
     4621 ttagtttttt aataatcaaa aatgagttat ctcaaataga gatagctcat tttttatggt
     4681 ataaaaaaat tataaaataa agggattttt ttatttaaaa attgctttaa gagatgatat
     4741 taataattta ttttcattag gttttaatat acatactcta aagaaatact catctagacc
     4801 ttcaaatgaa caacaatctc ttattattat tttttgtgga agcagttgtt ctcgaagcga
     4861 tttagcagtt aattcctttg ttttgatttt acacaatatg aaattacctt tagtattgta
     4921 tatatctaag ctttttatat tcttaagctc tttaagcagg taatctcttt cagtgttcat
     4981 taaggaaata gtatttgata taaaatctaa atcactaaac atgatttccc ccattttaga
     5041 agctattatg tttatattcc ataaatctag atttttattt atttcatttt tgacattagt
     5101 gttagatata agaccatatc caagccttat tccaggcgtt gaaaaaaatt ttgatgtacc
     5161 tcttataacg aataaattag aataatcatc aactaattga gtacaagagt atgtatctgt
     5221 atcagtaaac tctacataag tctcatcaat cattaaaaat gagtctgtat tttttaaaat
     5281 ttctctcact tctactttag taaatgcaaa tccagttgga ttactaggat tacagattac
     5341 taccaaatca taatctttag cattgatagt ttttataaga ttttctaaat ttatatgaaa
     5401 attatcttct tcttttgcaa agtatttttc tatagaacaa ttaattttag ataactcttt
     5461 ttcatactct gaataggctg gagagagtaa aagagcctgt ttaggattaa ttgtatgtat
     5521 aaatgatgaa attagttctg tagctccact gccaagtact atattatcta tagaacattt
     5581 gcagtaattg gatatagaag tttttaagtc tatgtaatca gggtcaggat acatagatac
     5641 catatctata ttgtttacaa tgtattgttt ggctaaactt gatgtaccaa aaggatttat
     5701 atttgagcta aaatccataa attcttcctt tgaaaagcca tacttactag ataaactata
     5761 taaattagct ccatgatttg tgctcataaa agtacctcca aaaataaaat tatataatcg
     5821 ataaaaataa ctgattaata tatgtacata tagattataa tacaaaggaa tcgttattcc
     5881 aaatttttaa tggaaaaagc aaaaaagtat ggataagctg ttaatttagg tctaaaaatg
     5941 atgaaaaaga ttttaattga tattttggta tgctataata tataagttaa tagaaggaaa
     6001 gcgggaggat acaaaatgta caaagttaca ggaaaaatgt tgacagaaga acaaataaaa
     6061 gaaaaagttt atgaattagg aaaaaagata gaagaagact ttaaaggcga agatttacta
     6121 gtggtaggta tacttaaagg agctagtgta tttgtttcag atttaataag atgtatagat
     6181 ttggatgtaa atatagattt tatgagtgtt acaagttatg gaaactcaac agagtcttct
     6241 ggaacagtta aaatattaaa agatttagat gtagatattg aaggaaagaa tgtgttgatt
     6301 gtagaggata taatagattc tggtttaaca ttaagtaatc tagttgcagc tttaaaaact
     6361 agaaatccaa aatctttaaa actatgtact ctacttgaca aacctcagag aagaaaagct
     6421 aatatacctg tagactatgt tggatttgtt atagaagaca aatttattgt tggttatgga
     6481 atagactatg ctgaaaagta tagaaattta ccttatatag gtatagttga ggatgtagaa
     6541 tagatttaaa tgtgaatcaa aagcactaat ttctaaggga gttagtgctt tttagttaat
     6601 tatataaaat tttagtaaag gagacatcta tatggatatt cagttaaata tattattatt
     6661 tttccaaaat ttaagaaatc caattttgaa ctttatattt cttgtcttta caataagcac
     6721 agaagcaccg ttattaatat taattacaac aattatatac tggtgtataa ataaaaaatg
     6781 tggacagaaa atactgtttg ctataattgg aaattttgtt gtaaatttag gcatcaaaga
     6841 gtttgtaaaa gctcccagac caataggaat aaaaggtctt gaatcattga gagtttctac
     6901 tgctggtggt tattcttttc caagtgctca tactcaaact gcaacaacat tttgggttag
     6961 tattatgact atatttaaaa agagatatct tcatttaata ggtgcagtta tggttttagg
     7021 agtaggattg tctaggctgt atttagctgt tcattggcca atagatgtga tagtgggttg
     7081 gatgttagga atatttttta cagttatatt tataaaaata tttgactata ttgatgataa
     7141 taaaaattat attttacttg tagttttact tataccattt ataatagtag ctatatttct
     7201 aaatagccct gaatattttg aaaaatttgg tataatagta ggatttgtat ttggatatat
     7261 ggttgaggat agatttgtta aatttaatac agataataat aacaaaaaaa taaattttag
     7321 caataaaaat aaggggagta aaaatataat tttttcaaga atatgtaggt ttttggttgg
     7381 gatagtaaca ataggaatgt tatatattgg cattaagttg tttatttcaa tgttaatagt
     7441 cactttaaat ataagtgact ctactatgag tataattttt atgaatttta ttaagaatac
     7501 agtagttgtt ttttatggaa tagcaggagc tccagcatta tttaaacttt taaaattaaa
     7561 ttgattttaa ggtatagagt caatagttta aattaaaaaa attaagggta gaaaagagtg
     7621 attatttgaa gaaggtagta ataataggtg gtggaccagc agggatgata gccgcatcaa
     7681 ctgcatgtga gaaaggctat gatgtaacat taattgagaa aaatcataaa ttaggaaaaa
     7741 aacttgcaat aacaggaaaa ggtagatgta atattactaa cgcttgtgaa atagaggaat
     7801 taatagaaaa tgtacctaca aatggaaaat ttctttatag tgctttttat acttttacaa
     7861 atgatgatgt aataagtatg tttaataatc ttggagtaaa gactaaaaca gaaagaggaa
     7921 aaagggtttt tccagagagt gataaagcct ttgatattgt aaatgctctt gaaagacaac
     7981 ttaaaagtaa gaaagtaaat atattattaa attcaaaagt agaaaaaata atatctaaaa
     8041 ataataaaat tgagaaagta attttaaacg ataaaaaaga aattaagtgt gattcagttg
     8101 tagtagcaac tggtggatta agttatccac ttactggttc tactggtgat ggatataaat
     8161 ttgcaatttc acaggggcac acaattatag atacaaaacc atctttaatt ggtatagaag
     8221 tacaagaaag ttttacaaaa gatttagaaa aattatcatt gagaaatgtt gaaataaggg
     8281 tgtttaatag caaacaaaag aaagtttata gtgattttgg agaacttgaa tttactagat
     8341 ttggtttaga tggacctata atcaagtctg caagttgtag aatgaaagat acaaggaaag
     8401 aaaattacac tatattatta gatttaaaac ctgcattaga tgaggaaaaa ctggataaaa
     8461 gagttcaaaa agattttcag aagtatacaa ataaaaaatt tgaaaaagca ttagatgatt
     8521 tacttccaaa aaaactaata cctataataa taaacttaag tgaaattaat gcaaatacgg
     8581 ttgttcacca aatttctaga gagcagagaa aaaacttagt ccatttatta aaaaatttaa
     8641 agtttacagt aaagagatac agacctattg aagaagctat aataacttct ggtggtgtaa
     8701 aagtaaacga aataaattca agcacaatgg aatcaaaatt agtagaaggt ttattttttg
     8761 ctggagaagt aatagatata gatgcatata caggtggatt caatttgcaa atagcttttt
     8821 ctactggata tttggcaggt tttaattgct aaaaattacc tgaattaaaa atgtttaaaa
     8881 tcacataata ttatcagcaa cacaaaataa attaaacaga aggaaggtaa tcaacatggc
     8941 aagtaacaat aacaacaaca gaacagtagt accaggagca aaagaggctt taaaccaaat
     9001 gaaattagaa atagctaatg aacttggtat gagtaattat caacaagttg ataaaggaaa
     9061 ccttactgca agagaaaatg gttatgtagg tggatacatg actaaaaaat tagttgaaat
     9121 ggctgaacaa caaatggctg gaaaaagcaa cagatagtta taaataatta tttataacta
     9181 ttggctatta gtttagctag agctatctca aatatagaaa gtaatttcta aattgagata
     9241 gctttttttt attaa
//
LOCUS       DAEPLF010000136         9214 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEPLF010000136 DAEPLF010000000
VERSION     DAEPLF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9214
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.136"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(219..557)
                     /locus_tag="KR314_002616"
     CDS             complement(219..557)
                     /locus_tag="KR314_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBH0328987.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(806..2122)
                     /gene="argH"
                     /locus_tag="KR314_002617"
     CDS             complement(806..2122)
                     /gene="argH"
                     /locus_tag="KR314_002617"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBH0328988.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     misc_binding    complement(2209..2420)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2499..3320)
                     /locus_tag="KR314_002618"
     CDS             complement(2499..3320)
                     /locus_tag="KR314_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0328989.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     gene            3681..4784
                     /locus_tag="KR314_002619"
     CDS             3681..4784
                     /locus_tag="KR314_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBH0328990.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCDEGINTVSYSSK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            complement(4842..7481)
                     /locus_tag="KR314_002620"
     CDS             complement(4842..7481)
                     /locus_tag="KR314_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBH0328991.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(7806..8453)
                     /locus_tag="KR314_002621"
     CDS             complement(7806..8453)
                     /locus_tag="KR314_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0328992.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            8714..>9214
                     /locus_tag="KR314_002622"
     CDS             8714..>9214
                     /locus_tag="KR314_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0328993.1"
                     /translation="MKGDNKVTEKQRLIINIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIY"
BASE COUNT         3034 a         1588 c         1026 g         3566 t
ORIGIN      
        1 ttctagtgct tttagtttta tttataatag cttcaaatat aaagaagaaa aaaagaaaaa
       61 aagctagaag taaaaaaaat atgaaaaagt aacaagtagt tggaaattat aataaaaaat
      121 ttataatata cttatcctta taaatctaaa agtactgaga taataattat aactattatc
      181 tcagtacttt taattttatg tttatgtatt ttttatacct atatattttt ttgtttttgt
      241 ttgtctttaa gcttacattt tgaacaattt cccagacatt catcaaccat atctagacat
      301 tcattaaatg ctaccttact actattttta tgccttacga ttaatatatc ttgtttttta
      361 gcttgagact caaccgttct agacatctta tgtgaaacag ttgatgtaag tatgacaact
      421 gcatctggtc ttcctataga attatttaat ttagatgaca tggtagtata tacctttgtc
      481 ttataacctt ttttcttggc cataaccata taatcttttt ccattctttc atgaccacca
      541 atcactaata agttcattag atttcctccc atttatttga tattgatttt cattattatt
      601 tatattatat ataatagtat ttagaaataa ttttgtcaat acttattgat attaattatc
      661 attttaataa taatttttta tttgtattta ttttaaatac tactcctttt aagtttctta
      721 cttttatata ataaaatctt acacaataaa aagctgtaaa tttaaatgat aacattcaaa
      781 tttacagctt ttaaaaataa atacattatt ttaaattctt ttttattccc ttatttaata
      841 tactctcata gtcgataaaa tcataaatat cttccttaaa agcatctgaa aacttatgta
      901 attcttctaa tgttaaatct tctattgctt ttttattatc ttcacaatat ataacgatac
      961 ttcctactat accatgtgca tctctaaaag caacattatt tttaaccaaa taatccgcaa
     1021 cttctgttgc atttaaaaaa ccatttttta ctgcttgttt cattttatcc tcatttacct
     1081 ttagtgtggc tatcattcta tccattattt gtatacacat ttcaagagta tgtacactat
     1141 cgaaaaaacc ttctttatct tcttgcatgt ctttattata tgctaatggt attcctttca
     1201 ttactgtaaa aagaccaaat aagtttccat atactctgcc agtcttacct cttataagct
     1261 ctgcaccatc tggatttttc ttctgaggca ttatactact tccagttgta tataaatcat
     1321 ctatctcaac aaatccaaat tcttgagaac tccaaagtat caactcttca cttaatctac
     1381 ttaagtgcat cattataatt gaaaaatcac tcattagctc tagtaaataa tctctatcac
     1441 ttactccatc cataaaatta tctactactt ttttaaatcc taattgttca caagttatac
     1501 ttctatctat atcatgcgta gttcctgcta aagctccaca gcctaaaggt gattcatcta
     1561 atatttctat agcatttttt attcttttct catctctatc aaacatacta taatatgcca
     1621 ttaagtgatg cttaaatgtc actacttgag ctctttgaag atgagtatag cctggcatca
     1681 tcactgggtt tttgtcagca acatctttta tagtagcttt aaattcagat attaaaccca
     1741 ccaaatcatt tgctttttct ttagcataca atctcatatc aactgcaact tggtcatttc
     1801 tactacgtgc tgtatgaagc ttcttgccaa catctccaat tctttgaatt aaatttatct
     1861 cagtaaaact atgtatatct tcatactctc catccaatac taactttcca ttttctacat
     1921 cttcaagtat cccttttaaa ccttctgtta tagactttcc ttcttcttct gttaaaagtc
     1981 cacattttac ttgcatatga acatgtgcta cacttccttc gatatctttt ttgtacagta
     2041 cacaatcata gccaaaagat ttattaaatt cttccataag ttgattttcc gcttttctaa
     2101 agcgtccgcc ccataatttc ataactttca cctcatattt taattttttg tattttaatt
     2161 ataagtcttg taactcaatg aaactaaaaa atctcttggt caaaaagacc aagagacgaa
     2221 atattccgtg ttaccactct agttggtaaa caatgtttac ccactctatt ctcttaaggc
     2281 gagattacct actaccatac tgaatttcaa gtagtattct ccaaagttct tttcaatata
     2341 aagtgttatc attaggctca caccatcccc caattcgctg agattttctc tatatctact
     2401 ctctttttca ctgaatctaa atttttatgt tttatctatt tgtattataa ttactatcta
     2461 caaaatagtc aacctatttt ttcctattat atactgtttt aagagctaag tgattctttt
     2521 aactctagga atttataaat tgcctttgca actccactgt cttgatttgt atctgtaaca
     2581 taatcagctt cttgtttaat caattcttct gcatttccca tagctacccc aagaccagca
     2641 aactttatca tttctatgtc attgtaattg tctccaaatg caattatatc ttctctgtct
     2701 atattcattt tttcagctaa aattttaact gcattacctt tatcaacacc ttcattcatg
     2761 acttcaatat tgtttggcca tgaagaagtt atctttattc cttgaacttt tctcaattct
     2821 tttcttattt catccaaaat agatggattt ttttcttcta tgattacaaa ttttagtata
     2881 tcttttttaa agatttcttc tctcaaatca tcttttacaa ttacttctgt ttcagagcct
     2941 aaaaatacac ttaatctatc ttcacgtcct tctatttttt tcgaaagcat agaatctata
     3001 ctattacatt ggtagtaaat atcgtattta tctaatacat ctaaaatgtt taaacatgtt
     3061 cttgtatcaa tcttatttga atatattata tttccatttt gctctcttat taatgaacca
     3121 ttacaagcta ttattggcat gtcctcttta agaaaatcta cattgcattt agctgaatcg
     3181 aaggctcttc ctgttgctaa agttacatta ataccgcttt ttataacttt ttgtagtgca
     3241 gttttatttt catctgtaac tttatgatta ttgcctaata aagttccatc catatctgtt
     3301 acaattaatt tatatcccat aatccaactc cataactttt tattgtatac ctatatttcc
     3361 ttaatataat ctaaagaatt acccttatta gattatatca taactttata cttaaactat
     3421 ttcaatttac aaaattaata acattcattt aacttatttt aagagcaaaa taaatcgttt
     3481 cttgtagtat ttttctgaat attttcaatt tatagtctta taatgctaga aattttacaa
     3541 tatatgatat ataatcatat taaataaagt gagctattat aatatttata ttcatttttt
     3601 actattatat attttcataa tattttaaat ctagcaactt ttataacatt tattattgac
     3661 taaataagga gtgataaagt atgaaactaa aaattaacaa agctattgaa atgcagttga
     3721 aaaaaagtta tgcaatagaa tctggacatt cagaagatgt attcgagata gactgtgatg
     3781 aaggtataaa taccgtgtct tactcaagca aagctgtaga agcttttaat gctttaaaat
     3841 ttgatatgat aagaggatat cctcattcaa ttgctttaaa agataatata gtcgattatt
     3901 ggaaagattt tatagcatta gatactgaca gaatttgttt ggcagatggt tctatacatg
     3961 ttatttacct tttaaataga ctatttattg aaaagggaga taaagtttta ggatattcac
     4021 ctcagttctc agaatatgag acagatataa aaatgcatgg tgcaacttat gattatgttc
     4081 ttttaaagaa agaggataat ttcaagttta atgaaaaaga atttattgaa aaaataaatc
     4141 cagaatataa agtaatttac atagacaatc caaacaatcc tacaggacaa attatacctt
     4201 tatcatcaat tgaaaatata gtaaaggaag cagctaaata tgatatagca gttatggtag
     4261 atgaagctta tggtgaatac atgccaaaag aaaattcagc tgtcaagctt ctaaataatt
     4321 atgataatgt aattgcttta aaaacatttt caaaaggatt tggtttagca ggactaagag
     4381 caggatatgc agttcttcca gaacaacttg ttagccctat taaaaagatt tctactcctt
     4441 acgaagtgtc tgaaatatct cgtagtattg cagcaaattt attagatgat gttcaattta
     4501 tagaagaatt aaaagaaaaa acaaaagata taaaaaatca attactaatt ccatggaaaa
     4561 accttaatat agctgaaact agtgatactg tatcaattat gacagttgaa cataaaaaca
     4621 aggacataga tttacaacaa gagtttgcta aattaaaaat aagagttata tctggaagtg
     4681 attttactgg acttgataaa aatttcataa gatttagaat gcctgaagaa aaagaattac
     4741 ctgaagttat aaaagcattc caaataatcg ataatataga ataaattaga ctatttataa
     4801 aaggcatatt gttaaaatca atatgccctt taattttttg attattttac ttcacttcta
     4861 tttattaatt tgttaagttc cattaatatt gatgaaatta atgctaaccc taggcaaatc
     4921 cccaactggt acaatccaaa cgttgttggt atagagaata ttcctcttag cactggtatt
     4981 agtgttaaag agtaaagccc aagacagact aaaactgctc ctataacgta tttattgctg
     5041 aaaaaaccta attttattac tgtttgatta ttagaacgtg caggtaatgt ttgcattatt
     5101 ctagctaata tcaatgttga aaatgccatt gctactccaa gttctggaga tacttgtaat
     5161 cctatatatt gtgcaattat tgtaactaca cctattatac aacctcttat agcaactgcc
     5221 ttcaatgttc ctcctgctag tatactttca tccgggtgtc taggaggttt attcattata
     5281 tttttttctg acttttcaaa ccctaatgca attgctggta aagagtcatt tactaagttt
     5341 ataaacaaaa gttgtaaagc tgtaaatgga tttgaccaac ctgcaaacac tgcaaatact
     5401 atggatacta tagctcctaa gtttcctgca aatagataag taattgattt tattatatta
     5461 ctgtatactg ttctaccaac ttcaacagca tttactatac ttgcaaagtt atcatcaacc
     5521 aataccatag cagaagcatc ttttgctaca tcagtaccac ttcccatacc aattcctata
     5581 tctgcttgtt ttaatgcagg tgcatcatta acaccatcac cagtcattgc tgttatatta
     5641 ttcttatgtt gccatgcttt tactatacga attttatttt ctggcgagac tctggcatat
     5701 acacttatat tttctagttt ttcatatagt tgttcttcac tcaaagcatc aagctctttt
     5761 ccagtaagag ccaagtctcc ttcttccatt ataccaatct cttttgcaat tgctgctgct
     5821 gttgttttat ggtcaccagt tatcataaca gtttttatac cagcactctt tgcctctttt
     5881 actgcatcca ttacttcttc tcttggtggg tctatcattg ccattattcc aaccaatgtc
     5941 atgtcatgtt cgtcatcaat tgaaggtata aaatcttctt ctgggacatc ttttattgca
     6001 aaagctaata ctctaagtgc tctatttgaa aactcttcat ttttatgttt atattcttct
     6061 attatctcat ctgttatatc tacttttgaa ccatttttca aagcatattt acatctacta
     6121 aacacaatat ctggagcacc ttttgtaaac attatataat ctccatatat actatttaca
     6181 gtagacataa gctttctgtc tgaatcaaat ggtatttcat ttattctgat atacttactt
     6241 cttaatttat cataatctaa atcattttta cttgaataat ttattaaagc cacttctgtt
     6301 gggtcaccaa cttctttacc atcactagtt atatgagagt cattacacaa tgtagaaacc
     6361 atagtcaata atttagcatg ataagaatag tttacttttt ctttatctaa ctcatcatta
     6421 ttagttccat acatataata atcaactact gtcattttat tttgtgtaag agtacctgtt
     6481 ttatcagtac atattacact tgttgaacct aaagtttcta ctgctggtaa ttttcttatt
     6541 atggcttgtt tttttgccat actatttgtt ccaacagcca atactatagt gactattgat
     6601 gatagagctt ctggtatagc tgctactgct actgctacag agaacatgaa tgaatctaaa
     6661 ataatttgtg tcatttctcc atttccagcc ataaagtaat ttcttgctac ttgaattata
     6721 aatattaaaa gagcaagtat tacaatacct acaccaagtt ttttactaaa atcctctatt
     6781 ttttgttgca atggtgtaag tttattttca gcactttcta aaagcccagc tattttaccc
     6841 atctcagtat ccatacctgt tccagtcact acaaaagaac ctctaccata aacaactaca
     6901 gcaccactaa ataccatatt tttctggtca cctaaactta catcttcact tattttttct
     6961 gtatgcttta aaactggttc tgattctcca gttaacatcc cttctacaac cttgagagat
     7021 tgtgcttcta taagccttcc atctgctggt atgtaatcgc cagcttctaa gaatacaata
     7081 tctccaacta ctagattttt tgctggaatt gtcatttttt tattgtctct tattaccttt
     7141 gcgtttggtg cagataaatt tttcaaactt tctaatgaac tttctgcttt ttttgtttgt
     7201 acaacaccca gaacagagtt tataataata actgcaaaaa ttattataga ctccactgtt
     7261 tcacctaaaa atatttgtac taaagcagct atcaatagaa ttattactaa tgggtcttta
     7321 aaactttcta ggaaaagctt ataagtagga accttatctg cttcttttaa ttcattagct
     7381 ccattttttt ccaacaactc ttctgctctt tctttactca aaccagataa acttgagtct
     7441 aaatttttaa ttacttcttg ataagtactt ttgtaagaca tttgttgccc tcctcttcaa
     7501 taaatttatt ttatgtttgg gggctcttat gtatacttga aatttaaatt tttttaaata
     7561 aaaattcctt gaataaaaaa agaccccaaa acaaacatac ctaatgctta ttttgaaggt
     7621 cttgctatca aggaatccct tgctaattaa ccgagtattt ctactgtctt gacgattaat
     7681 taccactttc gtgtaagcta ctccccttcg agtagttatc ttaaattgta tttacattat
     7741 atcactttac acagctaata gtctaggtta ttttttattt taacctaatt ttaacaaacc
     7801 atctcttaat ttatatgtaa atattcttct aaagtcaaat tattcattgc aaagtttttg
     7861 ctatgttcag ttccaacata tctatagtgc caagattcat acatatatcc tgttatattc
     7921 tctttacctt ctggatatct aagtataaaa ccatatttat atgcattttc atacaaccac
     7981 ttagcttctt ttgtattgtt gaacttttta tttgcccaac aagactggtc atctcctcca
     8041 atatcaaaag caagtccagt ttgatgctca ctctctccag gtttagctga aaatctattt
     8101 gcttcttttt ctccatgttc ttttacatat gatttaaata atctatcttg atattcataa
     8161 cttctatatg tactaaaagc ttttaaattt aatcctgact tttgagcatc tttattcatt
     8221 ttataaaaag catctctcgc ttcactgctt tcctgaggag aataatcttt gggcaaacta
     8281 tattttttat tcgctactat aatattgttt attgataata catttacgcc tttcctgtat
     8341 ttacttaatt cagctgtcat aattttactt ccttcgctag agctatcaat aaacatttct
     8401 acaaaaacta taaatagagc aaaaattatt gctacacaaa ttttcttttt catatactac
     8461 acctccataa ttggaatttg atacagaaaa atttcaagac attctctttc atactataat
     8521 cataccacta atttattgca acttctttaa acaattctta agaaactctt aagtttcttt
     8581 tttatacgac taaaaataac aacaaatttt tcaaaaattt catattacag tctatatatt
     8641 gatttttttt ccaaatgatg tataatatat ttattaaaac ttgtaggtac tatatagtac
     8701 taaacgtttt tgcgtgaaag gagataataa agtgacagaa aaacaaaggc tcattataaa
     8761 tattgcacaa aaaatttttg accaaaaagg ttttcaaaat acttcaatat cggatgttgt
     8821 aaaagagtgt aaaatgtcta aagctacttt ttacaagcat tttgaaacta aggaaagttt
     8881 tatctgcgaa ataatcaatt attatgatga aaagttttta gaaattatac attccataaa
     8941 cgaaaataat gacattccat catcagaaaa gttaaacaga aagataattg ctgtatggaa
     9001 aaatattttt tctagaacta ccataaatac ctatataaga gaaaattttt ctgaagagca
     9061 acgtaaggct acaagtaaat tgcaaacaaa atcacgagct aacttattga atgaatacaa
     9121 attaagctta tttgataact atggtgataa aatagaaaaa attatatttg atttagtttt
     9181 cttattggat gctctaattc atcagtttat atac
//
LOCUS       DAEPLF010000137         9207 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEPLF010000137 DAEPLF010000000
VERSION     DAEPLF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9207
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.137"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(60..713)
                     /locus_tag="KR314_002623"
     CDS             complement(60..713)
                     /locus_tag="KR314_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0328994.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKFMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(958..2823)
                     /locus_tag="KR314_002624"
     CDS             complement(958..2823)
                     /locus_tag="KR314_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HBH0328995.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDIPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVDENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2828..5248)
                     /locus_tag="KR314_002625"
     CDS             complement(2828..5248)
                     /locus_tag="KR314_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBH0328996.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNIDNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYEQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5262..6704)
                     /gene="glgA"
                     /locus_tag="KR314_002626"
     CDS             complement(5262..6704)
                     /gene="glgA"
                     /locus_tag="KR314_002626"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBH0328997.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNRDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6733..7863)
                     /gene="glgD"
                     /locus_tag="KR314_002627"
     CDS             complement(6733..7863)
                     /gene="glgD"
                     /locus_tag="KR314_002627"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBH0328998.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGS
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7865..9025)
                     /locus_tag="KR314_002628"
     CDS             complement(7865..9025)
                     /locus_tag="KR314_002628"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBH0328999.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKRSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         2903 a         1707 c          899 g         3698 t
ORIGIN      
        1 tttttccttt aatatttaat agtaaaataa caaaattata aaaaaaataa agagaaactt
       61 tataaaagct tctctttttt catctctgct ataggtatcg ttttccaatg ttctctcaaa
      121 actctttcaa cattttctaa atcattctcc ctcataaatt ttactacttc aatatgttga
      181 ttattgctgt atgtcaacat tttatataac tcatcttcat tatcactagt atatgacttt
      241 gacataaaat tatatcttaa agagttcaat agatttacta acacctgatt atctgattta
      301 gttatgtata tatcgtgaaa ttcattactt aattttaaat agctattgta atttttatac
      361 tttatagaga ccgccatcat ttctgttaat tcttccattt ttactaggtc tgattcagtt
      421 aaattttgta atgctgaaga accggctaac gcatccaata ctccaattac ggaataggtg
      481 tctaatttct ccttttcctg aaattctttt actacaaatc ccttattagg tattttttct
      541 agtaaatttt cacctgctaa cctagttaat gcttctctag ctggtgttct actaactccg
      601 atagatttac aaatctcaga ttcagtaatt ctctgattag cttcatactc tccattttgg
      661 attttctttg atatatagtc atatacttga gttgtaagtg gatctacctt cataagacgt
      721 tcctccatat taagattata aaagttaaat ctccctacaa ctttggtaga gagttcagtt
      781 ttatcttact aatttatata atatcacaat tatgatattt tttatacttc tttgtaatat
      841 tttgaaacac taaaattctt atgataattt atacaaaaaa agtagaaaaa gttttgataa
      901 aacaaaactc tttctacatt caccttattt tctatttatt tttctaaaaa aagatattta
      961 atttactcta aaaattctaa atgatttaga gtctaaatct actttcaact cattattttc
     1021 aatttgtata gtactatttg catttttaag actatacttt ataggtattt cctcaataaa
     1081 actactctct atacataatg atatactttc cacattttca cttctattta ctaaaattaa
     1141 cattctatca ttattttcat caactctaat aaaagaaaat acagaatcat tattagtata
     1201 tataaattct gtatttccag aacttaaaag tttattctta tttctaattt ttataacatc
     1261 tctataaaaa ttaagcatat cttcgtcttc atttttccat ggataagttc ttctattatc
     1321 tgggtcaaca cctccacaaa gacctgcttc atctccataa tatatatatg gtactccttc
     1381 aaatgtcatc tgtatagcta cagcaagctt cactaaatct acatcatcat ttaattccgt
     1441 tttagctctt gtaacatcat gtgttccaat taaatttaaa ttagacctaa atgattcttt
     1501 tggataattt tctttaatct gcatatatct attacaaagt tcatatgaat taatttctcc
     1561 ttttaagaaa gaaaacatgt tatttctaaa aggataaccc ataacagaat ctaattcttc
     1621 acctaataaa taacttcttc tctgaccata gctaatctta tttgatgcat cttcccatac
     1681 ttcacctata agaattgagt catcatctgc cttttttaat tcctttttta actctcttat
     1741 aaacttagtt ggtaattcgt ctgcaacatc tagtctccag cccttaatac ccatatttac
     1801 ccatttattt ataacgctat cattgtcata tattatataa tccatatagc tatgttctaa
     1861 ttcatttata tttggcaatg tcttaacatc ccaccaactt ttatactttt gaggaaattc
     1921 ttcaaacata taccatgaat aatatggaga ttctttagat tgatatgccc ctaaactatt
     1981 atagtttcca tacatattaa aatatttact atctgctcca gtatgactaa aaactccatc
     2041 taatactatt gatatacctt tttcttttgc tttatcaata agttctttaa atgtatcttc
     2101 atccccaaac attgggtcaa tttttttata gtcacctgtg tcatatttat gattactaga
     2161 agcttcaaat atagggctta gatatagtat acttacacct aattttttta aatatccaag
     2221 tttattgatt atccctctta aatttccacc atggaaatcc catcttataa catccccttg
     2281 actatccttt atgtacatag gtatatcttc ccaatttcca tatatgaaac tatttttttt
     2341 aggattatca accttaccac tacgatttcc attattgaat ctatctacaa atatatgata
     2401 aagtattcct tctttgtacc atgttggtac tttaaaatct ttgtaaacag tgatttgata
     2461 tttattaata tcactataat tatattcaca agaatatcca ttatctctgt tttttccata
     2521 aaataaagtc tttatctgac catccatttc aacatcaact ttaaagtaat aaaaataaac
     2581 acctgtacca tcaaactttt caatcttacc acaaaagcac tttccaagtt tatcattatc
     2641 taattcttca tttaaagtta ttgttctcat ttcttcttct ctatttattt ctagggaaat
     2701 agacttgata ttgtatccct catttacttt aacacaaatg gtcaattcct catcaaattt
     2761 taaagctcca aacggagcct taaaattttt atcccaagag ttatattcta ttacactttg
     2821 catttaatca caccctttta aaccaaatgt cttttgcata ctcagaaatt gttctatcag
     2881 aagaaaaaaa tccagagtta gctatattaa ctaaagacat tttattccat ttttctttat
     2941 ctctatataa cctgtttata tctgcttgag cttgaccata attttcaaaa tctctcaata
     3001 caaaatactc atcattgtaa gtagtgagtg aattataaat acttcttcct tcttcgccaa
     3061 gattaggaat aaatccattt attaaatcat ctaccaccct ttttatatcc atattagaat
     3121 gatataagtc aagtgaagaa tatccaccat atttattata atttagtact tgttctgcac
     3181 taagtccaaa taaaaacata ttttctttac caacctgttc acatatctct acatttgcgc
     3241 cgtcaagtgt agccaaagtt atagcaccat tcatcataaa cttcatatta cttgttccag
     3301 atgcttcctt agttgtagtt gaaatttgct cactaacatt tgctgctggt attattattt
     3361 ctgctaatga tactccataa ttctctaaaa atactacttt taacttgtcc tttactctaa
     3421 catcattatt tatagtactt gcaacagaat ttataaattt aattatacat ttagccaaat
     3481 aatatcctgg tgctgctttt gcaccaaata taaaagttct tgggtcaaga ttcaaattag
     3541 gattatcaag taattcatga tacatatgaa gtacattaaa tatattcagt aactgtcttt
     3601 tatacgcatg caatctctta acttgaacat caaatatcga acttgggtct acatttaaat
     3661 cgtatttatc ttttataaac tttgctagat ttgctttatt attgtatttt atatcatcaa
     3721 gtctttgtaa aacagaggaa tcatttttaa atttttcaat attttttaat tgtaaggtat
     3781 ccttcttcca tgaatcacca attaaatctg taattaaatt gctaagttga ggatttgaac
     3841 ttataagcca tcgcctatgt gcaattccat tagtcttatt attaaattta tttggttcat
     3901 cttgatagaa atcttttaat acctctgttt caagaatatg agtatgaagc ttagcaactc
     3961 catttacact atgacttgtg acaatacata agtttgccat attgatattg tcacagtcta
     4021 ttatactcat tctttttatt ctatcctgct catatccttt attatttaat tcctctacat
     4081 atcttctatt tatttcttct attatcatat aaattcttgg taatagttct ttcatcatac
     4141 ttacaggcca tttttccatt gcttctgaca ttatagtatg attagtatag gacactgttt
     4201 ttttagttat ttcccatgct tcatcccaag ataaattttc ttcatcaagc aaaattctca
     4261 taagttctgg tatgcataag gtaggatgtg tatcatttat atgaattgct accttatcga
     4321 ataaattatc aatatttaat ttgttctttt tatattttct tattatatct tgtatccctg
     4381 cacttacaaa aaagtattct tgtttcaacc tcaacaactt acctgcatag tttgaatcat
     4441 caggatataa tacctgtgat atttcctcag tataatactt atgttttaaa gcctcttcat
     4501 agctaccaga ataatttaga gcatttgagg taagttcacc aaaatctctc tttggtattt
     4561 cactcttaaa caatcttaaa gtatttatac attggttttg gtatccgatt attggtatat
     4621 cataaggcat tgccattaca ggaaggtagt ttttatgaat tacttttaaa tgactacctt
     4681 ccttgattaa ttctacctca ccaccaaact tcaccattgt tgcttcattt ggtcttacag
     4741 tttcccatgc atatcgtcct tctgtaagcc agttatcagg cacttctacc tgataaccat
     4801 ttacaaattt ttgttcaaac agaccatatt tataccttat tccatatcct tgaccactta
     4861 tgttcaaaga tgccatagaa tctaaaaagc aagctgctaa tctaccaagt ccaccatttc
     4921 ctaaggctgg gtctacttca gcttcaatta aatcatccaa gttgattcct aattcagata
     4981 aaccttctct aacttcttct tcaatattta aatttaataa attagatttt aactgtctcc
     5041 ctattaaaaa ttctaaacta aaataataaa cttctttttt cttatctaac tttgtagcaa
     5101 cccatttttt tgctatctca tctttcaata gactacataa tacatttaac aattgttcat
     5161 cagtagcttc cttgatagat tgtgcatata agctatacat ttttacttca aatttttgct
     5221 tgaatttctt tttgcttatt gttaccaaaa aaaccactcc tttaatcaca tatgtctcta
     5281 tatgtttcta tatactcctt agctgatttt ttccaactat tatctgtttt cattgcattt
     5341 tcaactaatt taatccattt ttctttgtcc ttaaatactt tgattgcatt tttcaaacag
     5401 aaaaacatct catgagcatt gtaattttta aaactaaatc cattgccttc tccagtaaat
     5461 ttattataag gtgttactgt atctctaagc cctccagtct ctctaactat tggtaaagaa
     5521 ccatatctca tagctaacat ttgacctata ccacaaggct caaataatga tggcattaag
     5581 aacatatcac ttgcagcata tatctgttga gccaatgatg cattaaaagt aattctagct
     5641 gatactttat ctgaaaaatt agaatcgtaa tagttgaaca ttgattgata ttgatgttct
     5701 ccagttccta aaacgacaat ctgtaaatct tctctcataa gttctggcat catgtatgct
     5761 attaaatcaa atcctttttg agaaactaat ctcgaaacta tacctatcaa cggaatagat
     5821 gaatcttttt taagtcccaa tatatcttgt aatctcaatt tattttctac ttttttgtca
     5881 attgagtcta catcataatg aacaaatata tctttatctt ttgcagggtc gtttaaatca
     5941 taatctatac catttaatat accttttaat tttcctgact cttttctaag taatccatct
     6001 aattgttcac cataaaaaga tgtttgtatt tcatttgcat aagtaggact aacagttata
     6061 attttatctg caaaatttat accagctttc ataaaattta ttgagccata atactctata
     6121 tctctattat caaagtattt ttgtggtaag tccaatatat catataaaac atgcttatca
     6181 aatacacctt gatattggag attatgtata gtatacatag tttttatatt tttatacccc
     6241 tcaagggttg catatctttc ttttagaatt aatggtaaca tccctgtatg ccaatcattt
     6301 atatgtatta catctggata aaaatcaatt ctacttatag cttctaaaac tgcatctgta
     6361 aagaaagtat atctttctgc atcatctcca tatccataga tactctttct ttcatcttct
     6421 cttctaaaat aaaattcatt atctataaag taaaatttta caccatccaa ttccatttct
     6481 aaaagaccac agtattgatt tctccatcca acacgtacac taaacacagc tatttctttt
     6541 agttgatctt ttaaataact tggtatagtt gaatatttgg gcattattac ccttacatct
     6601 acaccttctt tttttaattc tttaggcaat gcataagaaa catctcctaa tcctccagtt
     6661 tttgcaaagg gccaacactc tgctgttaca taaaaaacct tcattagtca ttcccccttc
     6721 taaaaaattt atttatatat ttgtattctt ttccactaca agaggatatt tttcatctcc
     6781 tcttaattct ttcccatttg atacactcgt atttttatct aatattgcat tatatagttt
     6841 tgcatttgac tttattacac aattttgcat tactattgag ttctctataa ctgttccttt
     6901 ttcaacattt acctttctaa atatgatact atcttttaca gtaccttcta ttaaacaacc
     6961 agatgctata aagctatttt ctactttagc actatctgta taaattgtag gtgattcatt
     7021 cttttctttt gtaaatatct ttctttcaga gtataataat tcatctgcaa cttctaaatt
     7081 taataaatct ttgctcattt caaaatatga ttcaacagaa tttacacatt ttagatatcc
     7141 tgtatattca taagctccta ttttcatatt ttctgcttct tcaataataa attcttctaa
     7201 ataacaataa ctgcctttat ttacaatgtt atatatacat tcaacaaaat cttcacgctt
     7261 cataatatat gtctccatac atatgtttgc tctagggaat ttgcctaaat tatttcctac
     7321 atttattacc ctattgttac tatctaaatt tagtgttaag ttaccagcaa aatctttatt
     7381 tgcattattt acatttttat atattatagt tatatcatta tgagattttt tatggtattc
     7441 caatgcttga gaataattta aattgcatag catatagctt ggagctatta aaatatattc
     7501 ttcttcactc ttttctatat aatctagatt agctaagata gtatatatat caccttctct
     7561 atgtgagtaa gtatttctat acttagtgtt ttctggacta aaaattgata ggccaccttt
     7621 ttttctactt aaatcccaat ctttaccact acctatatgg tctgtcaatg atctgtattt
     7681 ttcttttgcg aatattccaa ctttagtgat tcctgaattt atcatattag ataaagcaaa
     7741 gtctattatt ctatatcttc cagctattgg tgtagatgct attggtcttc catagttaag
     7801 tttgtttatt gctgggtctc cttttttgtt taaatttatt atccctaaac attcatttct
     7861 catcttaaag acctccttct attaatacgc atctaggttc gatattttca tactcagaaa
     7921 ctacatttat ttcattattt acattcttta tctgaacatt atcatttata gttgcacact
     7981 cacctatcat agctttttct atagttacat tttcacctat tacaacattt ggcattatta
     8041 ctgaatcaat tactttactt cctttaccaa caacaactcc atgtgataat acagagttgc
     8101 ctacttcacc taaaattcta caaccatcag ctatcattga tctatgaaca tttgcatttt
     8161 tacctatata ttgtggaggc attgccatag tatttgtata tatcttccat ttagggtctg
     8221 ctaaatcaag tgtttctgga gattttatta tatccatatt agcatcccat agactttgta
     8281 tagttcctac atctctccag tatcctttaa atggataagc atacattcca acattatctt
     8341 ctaacatttt aggtattaaa ttttttccaa aatcatcata atttatttct ggattttttt
     8401 ctgcttcctt aaaataattt ctcaacattt tccaatcaaa tatgtatact cccatagatg
     8461 ccaaattgct cttaggttca cttggttttt cttcaaattc atagatactt gaatcttcat
     8521 ttgtattcat tatcccaaat cttgaagctt catcccaagg aacttcaatc actgctattg
     8581 tagctttaga gcgtttttcc ttatgaaatt ttaacatttt gctataatcc atcttgtata
     8641 tatggtcacc agataatatc aatacatatt ctggattata ggtatctaca aagtccatat
     8701 tttgatatat cgcatgtgct gtaccattat accaatttcc ttctttttca ttcataaatg
     8761 gttgtaaaac atagacacca ccatttattc tgtccaaatc ccaatgactt cccatgccta
     8821 tatgtgaatt taatatcaac ggtctatatt gagttaaaac acctacagta tcaataccag
     8881 agtttgaaca attacttaac acaaaatcta taattctata cttacctcca aatgatacag
     8941 ctggtttggc aattcttttt gtaaaaacac ctaatcttga accttgccct cctgccaaaa
     9001 tcatagctaa catctctttt ttcatttaat gacactcccc tcatctagta attaatattt
     9061 ataattgcat ttttactttt ataaacttta tcagtcaata aatgccgtgt ataaatgttg
     9121 cttaaataat accttaataa aaataaaata atatcaacta tacaattgat ataatgtata
     9181 ccccataatg aacttaccga cgtatac
//
LOCUS       DAEPLF010000138         9184 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEPLF010000138 DAEPLF010000000
VERSION     DAEPLF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9184
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.138"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            381..1580
                     /locus_tag="KR314_002629"
     CDS             381..1580
                     /locus_tag="KR314_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329000.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYANILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQDIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            1589..2401
                     /locus_tag="KR314_002630"
     CDS             1589..2401
                     /locus_tag="KR314_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329001.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFIFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVLEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            2412..3680
                     /locus_tag="KR314_002631"
     CDS             2412..3680
                     /locus_tag="KR314_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329002.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNKNPKSRIRNDYLNIDKKYSDFVNDKG
                     KELIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     YQQNINEIKFSIGSKLYLEIVNNKSKNGDIEGHDSYSNLIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMIKNNKLNLIVTDMSNSIINLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGMKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
     gene            4046..5227
                     /locus_tag="KR314_002632"
     CDS             4046..5227
                     /locus_tag="KR314_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0329003.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQVDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(5369..6286)
                     /locus_tag="KR314_002633"
     CDS             complement(5369..6286)
                     /locus_tag="KR314_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329004.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            6539..7333
                     /locus_tag="KR314_002634"
     CDS             6539..7333
                     /locus_tag="KR314_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329005.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            7334..>9184
                     /locus_tag="KR314_002635"
     CDS             7334..>9184
                     /locus_tag="KR314_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0329006.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFACYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEELFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSI"
BASE COUNT         3657 a          912 c         1447 g         3168 t
ORIGIN      
        1 ataatcaata tgaactttgc caaagattta tattatttaa acttgaagag tataccaatt
       61 gattttatct cttttatggc tatttatgta tttttactac ttatagctat ttctattgta
      121 aattgctgta tattatttat cttttcctat ataaataaat tttatgtaat tttaatcact
      181 ttgctgatta tagcaggctt atatttttca acaatatata taaatataat caatttattt
      241 agaaaatatt ataatcttat aggatttcaa tttaacaatt tagtagcagg gttattaagt
      301 tttttaatta taatgtttat tctaaatgga atatcttatt tgctaattaa aaagaaagta
      361 tctctttaag gaggggtggt atgaaattaa aatctaaaat atctatactt attatatttt
      421 tgactttagc aatatatttt agtataggtt tattaaataa tgaagataat ttaaagataa
      481 aaactataaa aggtgactct aatgaattag accatataaa cataaatttt ggatatgctt
      541 tgaatggata tataaattca tattatacaa taaatggaag taacaaaact aaaaagactg
      601 aattaataaa actacaaaag gatagtaaga acacaaataa tttaattgat gtatctagtg
      661 gttcagattt aattgctcat gttggatttg aaatgaatga ggagtcaaaa gatataaact
      721 ttgtgataga tgagaaaaaa gaagatttag aaaaaggtgt atttccatca gaaggtagtg
      781 ataaagaaat tgaatatgaa aaattagata tgaataattt tacaaggaca agatataaag
      841 tagataaaag tacagatgat tattatgcaa atatactagc atcaaaaaga tataaaaaat
      901 acttatatgt atctgtactt tatgctacaa gtgattcatc agaaaataac aaagaatatt
      961 caagtactat agatataata aaaattaatc cagaaaataa aaaaatcaag actataaaaa
     1021 gtataaattt aagtaattat ttttccgaga aataccaaga catagagaac ataggtgtaa
     1081 gttatgctag caaatatgag gataagatgt atattttagt aaatgtacta aatacaaaca
     1141 taggtgataa tagtaattct aaatttcatt tacttgagta taatcttgaa gaaaataagg
     1201 aaagcataat ggaattaaaa actgatagca aaatggcttt agcaaaagca aagttagaag
     1261 ctaataaatt atatatggta atgtcgaatg attatgataa tagttcaatt acagagttaa
     1321 atttacttac ctataatata ttagataaaa gcttagatga agataaatat gttttagata
     1381 atagagaaaa aagaggaatt agaagtattg aaattgattc aaataaaatc gttgcatata
     1441 catcaaggct aaactctata ttatcttggg ataataaatt tatgctatat ataataaata
     1501 aaaattctaa ggaaattgta tatgaaggag aattacaatt taacagttca aatagtatgt
     1561 tagaatttga aataaagtag gtgagttgat gaaagattta ataagattat tcgaatttga
     1621 attaaaaaga aatataaaga attatatatt tataataatt tgttgttgtt cttttgtaat
     1681 tttaaacata gtaaaaaatt taaatgacta taattacata atagaaaatg cagttaaaag
     1741 tgaacagtta acaaaaatcg gaaatacgat agaaacggta aatgttacag gtttctcaag
     1801 ctttagaaat ataatgggtt ctacagaatc gtggtttata tttggtataa ttgcatgtat
     1861 ttgctatgct ttttttattt ggtatagaga ctttaatggt aggagtaaat ctatatatac
     1921 acttataatg ctacctaaaa atagaatcaa tatttatata tctaaattat taaatatatt
     1981 atttctagtg tatagctata cagtagtact tacaatatct ttgtttattg caagtaagtt
     2041 gttgcctaga catatgcttg gtaatgtcac aaactatggt tttgtacaag aaacaatata
     2101 tgagttaaaa atgttacttc catatagctt tgaaatacta tttgtagagt atatattttt
     2161 attgatagga tttgtatctg tagtatttac atcaattctt ataaataaat ctatttataa
     2221 agtaagcact ttaataagtt ttctattttt agttttggaa attttagtgt ttataatttc
     2281 tatagaattt attatacctt atgaatacag tgatgtattt actgttctat attcaagtat
     2341 aaacatattt gtatgtagtc ttatatctaa taaacttttg aagcaaaaga ttgactttta
     2401 ggagggatat tatgaaagca aaaaatatag gaaaatatac aatatgtttt ataatagtat
     2461 gttttttaat gattactatg accttaatat tgagttattt taataaggat aaactatctg
     2521 taaaaacttt aaaaggcaat gtaaaagata tagggagagt aagtgtagta tatacaccta
     2581 atataagcaa attttcttat gaggaggtaa ttataagtaa aaatggagta agaaataata
     2641 aaaaccccaa atcaagaatt agaaatgatt atttaaatat tgacaaaaaa tacagtgatt
     2701 ttgttaatga taaaggaaaa gaacttatat ataaagatga tttgaatata ggagaagcgt
     2761 atacctctgt taagtctgat ttagtacaag gaatgaatgc tttaaatgat tgtataaagg
     2821 tttcaaataa aaatttaaaa agtggggtag aagaaaattt tgatattaat ttaaataagt
     2881 ctgattttaa agaaaacagt tggaaaacct tgtttaataa aatatataaa ggcaatttat
     2941 atatggtaat tagtgaaact aatatttcag gttctaaggt caataatatt tgtatattaa
     3001 agatagatat aaaaaataag tcttataaat tattaaatag atttaattta ggattacaga
     3061 atggatatca acaaaatatc aatgaaatta aattttctat tggaagtaaa ttatacttag
     3121 aaatagtaaa taataaaagt aaaaatggag atattgaagg acatgatagt tactctaacc
     3181 ttattatata tgatatagaa gataacactt ttaatataag taataaattt atggataatc
     3241 taaacaaaga aaagaatgca aatccaaagg agattggctt attaagtaaa actaagctag
     3301 attatatgat aaaaaataat aaattgaatc ttatagttac ggatatgagt aattccataa
     3361 tcaatcttgt ttatgatatt aattctaatg agattaaatt agaagattat gagatacttg
     3421 atttaaaagt agattataat gaagcatcat ctggagttag gaaagttaag ttactggatg
     3481 ataagattta ttctatttca ggtatgaaga acagtatatc aggagaatta aaaggattta
     3541 aagggattgt tgatgtagtc ggaactaagc cagtaaaatt tcaagtattt gatatgaaac
     3601 ttaaaaagaa tatttatgaa gctgagctaa taaatggaga tataagttta caagaaaagt
     3661 tattttttgt tgcaaattaa gtattagaat taattttaga actttaaact tttatatgta
     3721 tcacagataa gtataagtaa gggagtgtct caaaataaac tttttagttc atgagacact
     3781 ttttttatat gaaatcaaat atattttcat tgtttagatt tattttgata atgaaaaaaa
     3841 gattatcttt attttaagaa agacatttat ttataattat atacaaaaat attttttaat
     3901 gtttttgtgt ataattataa caatttaatt tatattaatg cacttaaatt tgaaatagaa
     3961 tgcttatagt ggtaaaatta attattagaa tattagaaat aataaataaa taatacattg
     4021 tacttaaaag ggggagaaat ttaagatgaa caaaaaatat attaaagcag ggaatttaat
     4081 tgatggaaca ggaaatagta ttatgcaaaa taagggtata attattgatg gagatactat
     4141 agttgaaatt gaagatataa gagatgattt aaatggatat gatgtgtacg attacttaga
     4201 taaaactgtt atgccaggca ttatgaattg tcatgttcac ttgacaatgg agccagttgg
     4261 aaatccaaag atatattatg ataatgtatc agatgtagag cttgtagtaa atacgataaa
     4321 acaagttgat gcttatttgg attcaggagt aacatatatt agaagcttag gttgtcccaa
     4381 atatgtggat gtacaactta aaaatcttat agacaaaggg tctatagatg gtcctggtat
     4441 agttacttca ggtccagtta tatgtatgac aggagggcat ggatattatt ttggtattga
     4501 aagtgatgga gtggatgaat gtagaaaatc agccagaaca gttttaaaaa atggagtaga
     4561 ttgtgtaaag ataatggcaa ctggaggtgt aacaactgat ggagttgagc cagggtcacc
     4621 acaattaact ttggaggaaa tgaaggctgc tgtagatgaa gctataaaag caggtaaaat
     4681 aactgccaca catgcacaag gaagaactgg tataaaaaat gcagttcttg caggaattac
     4741 ttctatagaa catggtgttt atttagatga tgaaataatt gatttgatgc ttgaaagagg
     4801 aacttactta gttccaactg tggctgcacc atattttatt ctaaatgcag gaaaagaaag
     4861 tggaataact gaagctactc ttagaaagtg tgagattgtt gccattccac ataaagaatc
     4921 attcctaaaa gcatataaaa aaggaattaa aattgcagtt ggttctgatg caggaacttc
     4981 ttataatgaa catggaaaaa catattatga aatgaagcta atgtcagatt atggtatgga
     5041 taatatggat ataatagttg ctgctacaaa aacagcatct gaacttttaa gaattgataa
     5101 aaattatgga actctagaaa aaggaaagaa agcagatgtt atagttgtaa atggaaatcc
     5161 agtagagaat atagacgtac ttgcagatgt gttagcagta tttaaacttg gtaaaaaagt
     5221 tagataaata gtataaaaat aaaaaagagt gtctaaagaa atttttaaat aattatagac
     5281 actctttttt attagtaata attaaatctt ttatacttta gaaaatagat tctattttat
     5341 atttagatat aactctttaa aatcattctt atgcaatata atctatagaa tctacacata
     5401 caaagtatat tcttgaatca ttagaatttc caagtactat aacatcattt ttcttaccta
     5461 gaacttcaac accagttaga accaaactac cagctactac agtaacttga ttatttatat
     5521 ttgacatata aagattatct aggatacctt tattacaaca gcaatcatca caatcgcagt
     5581 tacaattaca ttctttgtaa tgttttttat caagatagta acctaaagat gctatgaaat
     5641 cttcataatc tgcaggtgta atatcaaatg caacagcttt aagattacac aatgatgcta
     5701 aatctacgtt tgctaatgga gtagatgcag caagtataaa atcaattata gcagcaagta
     5761 ttgcatctaa tatagcccca aggtctatcg taggaggaat aactgcatca ataagtgcaa
     5821 ttaattcagc tactcctggt atagttccta tagtattaat taatccctca agagttaaag
     5881 ggactggaat aggataagat actctaccag ctatatctat taaatcacag ttacaagcag
     5941 aaaatctttc aaaagtacca tcaagtccag ataaattatc ttttggagga gctgaaagat
     6001 ctatacctac caaattagca cctactataa agaaatctga gataaaagca aattgattaa
     6061 agtttacaaa aggtcttaga gcatcatatc ttagaagttc taaggcctct ctcatacttt
     6121 ttttacaaca acattgtttt attttaggtt ctttatattt atttggataa ttatagtatc
     6181 tatcagctac ttgataattt tcatagtatc tttcatttgt atttggataa tcttcctcat
     6241 attcttgtgt aaatctaaag tcctctctac atttattatt ttccactaaa tacactcctt
     6301 ttatatttta atttaaatgt atagataatt aagcttatct atatagtaat attatattta
     6361 taatgtaaaa aaatgttaca aaagatatta taaacgaata aaaaataata atttgtcaga
     6421 taaacttgta aatttttatg aataatccat tctaaaacaa aaaactgtgt ataaaaaagg
     6481 tttatatgtt atatttatat aaatataaca tatgaacgag aaaggagtgg tttgataaat
     6541 gattgaggta gataaattgt gtaagtcttt tacaagggtt gtaaaagatg ataagaataa
     6601 aagttcaata agaaagctaa agaaagtaaa aactaaaaaa gaagaatttt ttgctgtaaa
     6661 taatgtttca tttaaagtta tggaaggtga aattgttgga atattaggtc caaatggtgc
     6721 aggaaagact acattactta gaatgttagg tggcatatta acaccaacat caggaagtat
     6781 aaatatttct ggatatgatt attcaataga tagaaattca gcaaaaaaag aaataggtta
     6841 tctctctgga aatacaaaac tatatggaag gctttctccg agagaacttc taacaacttt
     6901 tgcaagtcta tatgagatgt caaaagaaga tatagaagaa tcaattgaaa atattgtaaa
     6961 aataatggat atgagtgaat ttatagataa tagaattgaa aatctatcaa ctggtcagac
     7021 tcaaagaaca tctatagccc gttgtttaat acattctcca aaagtatata tttttgatga
     7081 gccaacacta ggtctggatg ttttaagtag tgcatctatt atagatttta tgaagaatga
     7141 aaaactaaga ggaaaaactg ttctttactc aactcattat atggaagaag cagaaacttt
     7201 atgtgacaag atttttatga ttcacaatgg gaaaataatt gcatcaggaa caccacagag
     7261 tttaaaagag gaatctggag taaataattt gagagatgta ttcataaaac ttgccagaaa
     7321 ggaggacatt taaatgagaa gtaagattgt aaaatacata tttaaaaaag aaatgttaga
     7381 catattgaga gataaaaaga ctttatttat gatgattgtt gtgccacttc ttttatatcc
     7441 tttacttatg ttattaatga ttcaagtaat gaatatgtct gcaagtagta tgaatagtaa
     7501 ggacattaat atagctttta ataataaacc taataaggtg ttagtatcca tgattgaaga
     7561 tgataattta gaaaaagata ctaaaaatac ttcaaatgat aaaggcaaaa taagagtgtt
     7621 agatgtagat aactataaaa agtcattaga agaaaataaa attgatgctt atataaagat
     7681 ggaagaaaaa aaatcatcta taaattatca aatatatata aattcttctc aagaaaattc
     7741 ttctaatgca ctaaaaagaa ttgaaactgt tttggataaa tataaaagat ttaccataga
     7801 aaaaacttta tctgaaaaag gattagatgt acaagctact ttagaaccaa taacatatag
     7861 cactgtagat gttgcaaaga cggaagaagt ggcaggatac tttttaggtc agatacttcc
     7921 atttatatta ataataggag ttttactagg ttcaatatat ccagcaatag atgttatggc
     7981 tggagaaaag gaaagaggta cattagaaac attatttaca ctcccaatat caaacttaga
     8041 actggttatg ggaaaatata tggcagtttc tcttagtgca atagtaacag caattttaaa
     8101 cattttatct atgggactga ctatggcatt tattttagtt agtggaggta ttagtagcca
     8161 atttggcttt ggaaacttta attatggaga cttagtctta ccaattataa ctactttaat
     8221 ttgcatatgt cttttttcaa tggtagtttc agcaataagt atgtgtgtat gttcattagc
     8281 aaaaagtttt aaagatgctc aaaactacat aacacctctt atgttaatta ttatgatacc
     8341 atcatatgtt tctatgatac caaatatagt attggataga gtgacagctg taatacctgt
     8401 agtaaatata tctttactta taaaaagtgt attatctttt aaaagcgatt taaatttaat
     8461 agccttagtt ttagcatcaa atgtagcctt tgttatattg gcaatcatac tactgtctaa
     8521 gatgttcaat tctgaggaaa tactttttgg taatagtaag agtttttctt tcttagagaa
     8581 aagaagtaat ataaaaaaag ggtctatacc aactgtaagt gatggtgtta ttttatatgc
     8641 agtaggatta gttctattga tttatgttgg ttctttagtt caaattaaat ttggtatggc
     8701 aggtattata atgacacaat tcatgataat ttctttacca ttattatttg catgctatat
     8761 taaagctgat tttaaagagg tatttagctt gacagttcct aaattaaagc atgtgtttgg
     8821 gagcattgtt ctttggattg gtggatacat acttattatg attataacac agctaatttt
     8881 attcttattt cctcaaaata tggaagttgc tgaaagttta aataaggctc tttttatcaa
     8941 agatagtgtt ttactgaatt tactagcaat agctttatta ccagctatat gtgaagaact
     9001 gttttttagg ggatttatat tttcttcatt tagtaaatca aaagataaaa ataagtctat
     9061 caaactcgct ataatatgta gtggggtact atttggtatt atgcatatgg attttattag
     9121 aataattcca acatcaatat taggtattat atttgcatac agtgtgtata aatctggttc
     9181 aata
//
LOCUS       DAEPLF010000139         9070 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEPLF010000139 DAEPLF010000000
VERSION     DAEPLF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.139"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            81..266
                     /locus_tag="KR314_002636"
     CDS             81..266
                     /locus_tag="KR314_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBH0329007.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEVYKILQKLDIDFERVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            complement(331..3105)
                     /locus_tag="KR314_002637"
     CDS             complement(331..3105)
                     /locus_tag="KR314_002637"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBH0329008.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAQALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRSMNSNSKN"
     gene            complement(3277..4533)
                     /locus_tag="KR314_002638"
     CDS             complement(3277..4533)
                     /locus_tag="KR314_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBH0329009.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(4605..6107)
                     /locus_tag="KR314_002639"
     CDS             complement(4605..6107)
                     /locus_tag="KR314_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBH0329010.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(6198..7388)
                     /locus_tag="KR314_002640"
     CDS             complement(6198..7388)
                     /locus_tag="KR314_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0329011.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIEYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYTIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            complement(7627..8715)
                     /locus_tag="KR314_002641"
     CDS             complement(7627..8715)
                     /locus_tag="KR314_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329012.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT         2919 a         1687 c         1063 g         3401 t
ORIGIN      
        1 tttatactat atttatgttt ttataacctt atgatacact ataactataa gattttaaac
       61 ggaaaggaat gaaataaatc atgtttttta tcagtgatat ttacacaaaa tcacctatta
      121 aatttgatac tccattgcaa aaagaggtat acaaaatatt acaaaagtta gatattgact
      181 ttgaacgtgt tgacacagat gaagcaatta caatggaaga ttgtgttcag attaataaaa
      241 aattaaatat gaaaatggta atataaaaca acattaaaca aacaaaaacc gcaaagttac
      301 aatatatagt actttgcggt ttcataagaa ttaattttta ctattagagt tcatacttct
      361 actatttttc attgttctaa gtattagctt tacaaattca tttgacaata atatagtgga
      421 tgcaaatagt attattttta tccacatatc aaagtccaaa gaaacagaat taaagaaact
      481 ctggaacact tgtgtaaata ttatttgaat tatccctgta actactatta tttgcaatgc
      541 cagtttattt ttaaagaaat ttggtattgt actatttaaa ccaaattctc tacaatttaa
      601 tgcattaaaa agggcactga acgcaaataa agaaaatata acagtacctt gttcttctga
      661 tgtagctccc aatatattaa aagctgattg agtaaataca attgtaatta taagaatagc
      721 atttataata atatttacaa acatagacct agctattatt ccagaatgtc tatttattgg
      781 ttttcttttc aatacataat ctctaacagg ttctaatcct aatgctaggg ctggaggacc
      841 atccattatt atatttaccc ataataattg tattgtagta aatggcatat ccttaccagt
      901 aagttgagat attatagcaa ttataaatgc tactatattt acagtaagct gaaactgtat
      961 aaatctttga aagttctcat atatacctct tccccatttt attccctcta ctatagtact
     1021 aaagctatca tcagtcaata ttatatctgc tgcattttta gatacttctg ttcctgaaat
     1081 acccattgca attccaacat ctgcttgact aagagctggg gcatcattta taccatcacc
     1141 tgttacagct acaacttcgc cacttttttg aagcgctgaa actattctca tttttgtatc
     1201 tggtttactt cttgcaacaa tagatattcc tttaatttct tctctcagtt ctttatctgt
     1261 caatacatct atatatgttg cttcaactgc ctttttccca tcatttaata atcctaattc
     1321 atttccaata gccgttgctg tatttatatt atctcctgta agcattttta catctactcc
     1381 tgcattaaat gctttatcaa tggaatcttt gaccccttct ctcaatgggt ccactattcc
     1441 tacaaatcca ctaaaaacta aattatcttc cttcatataa cttcttgaat ttcctattaa
     1501 attcaattca ccttctgaag tcattgcaac ttctgtttta gaattactca tttttttata
     1561 agcaaatcct aatgttctca tagatttaat ttgtaacttc ttaatctcat ctaaaatact
     1621 tttttctact tttggagtta ttggaacaat attttttcct tgttgtacat aagaacattt
     1681 ttttaataat acttctggag caccttttga aagtaatata tctgaatcct cttgacgaat
     1741 taatgttgac atcttctttt cttctgaact aaatggtatt tgtgatatca aatacgtttg
     1801 ttttctcatt tcattatagt tcttatcatt atgatataaa agtaatgcac actctgttgc
     1861 acttcctata tatttaaatg aattatcttc tttttctata tctgcagttg aatttactat
     1921 acagttttgt tcaaaatagc tgttactttg atactctcct ccacttatat atttaccatc
     1981 aacatatgct acctctacca tcattttgtt ttgtgttaaa gtccctgttt tgtcggaaca
     2041 tattacagaa acagaaccta tagtttcaca ggcttctttc tttgttacta atgcatttat
     2101 ctttgccatc ttttgcattg ttatagctaa ggtaatattt atcatagttg gcaaaccttc
     2161 tggaacagtt gcaactatta atgcaataca aaccatatat gcatttttag caggttctaa
     2221 tgattgtaag aaagataata tactgcttgt atctaaaatt aacatacctt ttctctgcat
     2281 ctgtataatc ataaacatac acaataaagt agctattacg cctgatattc ctgctatttt
     2341 agcacccaac ttccctaatt taatttgaag tggagtttgc tggtcttcat ttgataaatt
     2401 ctgtgctatc ttacccatct ctgtcttatc accagtatga gtgacaacca ttatgcccct
     2461 accatatgct acaagagtcc ctccaaatac catattgact tgctttgcag gtattgcatc
     2521 ctgttcgatt atttctgttt tagaataaac tacttccata tctaatacag catcagcatt
     2581 cttagctaca tcatcagatt ctcctgttag catatcttct ctaagtttca aatttataga
     2641 ctgtataagt cttccatctg ctggtatcat atctccagtt tctatatata caatatctcc
     2701 tggtactaaa tcattttttg atattttaat tatctttcca tttctaagta cttttacttc
     2761 tatattttct gttaattttg acagggcctg agctgctttt ttagattttc cttcagttat
     2821 tatgcctata gaaattccaa taaaaatagc ccctaaaata cctaatgcat catgtaattc
     2881 tcccacaaaa gaacttatta ctgctgctcc tatgagaatt aatatcatag gttctgtcaa
     2941 actttctccc aactcgtccc aaaatgttct accttctttt atagtaaatt catttaatcc
     3001 atatctaagc ttcctttctt ctacttcatt atcatcaagt ccaatttctg gatttgtttt
     3061 taagtatttt aatacttctt tggtaggttt attataatac ctcataactt tatccccctt
     3121 gttaattatc tataaggaat tatatgctta tgatagcttg tatattactt ttcaaaaaaa
     3181 ataaggtgtt gattaatcaa caccttatag tggttatata ttattaaatt ttatgttaca
     3241 ggctttataa attgtgagct atacaaatca aaatatttaa tcttcaaaat acccttctgg
     3301 atataatggg aacttagata taagttcttg tgcctctgct ttacattcat ctaatacttc
     3361 tttattatca atattttctg ctaccttatt cattattcct gctatctgta taacttcttt
     3421 ttctttcaga cctctttgag atattgatgt aagacctata cgaacaccac tagttacaaa
     3481 tggtgattgt gggtcgtcag gtataacatt tttattaact gttattccta catattcaag
     3541 agctttttca aattgttttc ctgtaagatt cttacttctt aagtcaacca ttactatatg
     3601 attatcagta cctccactta caatactaaa accatgtgaa atcagttcag aagctagagt
     3661 ctgagcattt actacaactt gctccattat ctttttaaat ttatctgtac ttaaatataa
     3721 tagtgaaaag gcttttgctg caactgtatt aagatgtata gaccctaatg ttcctggaaa
     3781 aacaccttta tcgagtttct ttgcatgttc tgctttacaa agtacaatac cacttcttgg
     3841 tccacagaaa gtctttgtcg tagatgaaga aacaaaatct gcataaggta caggactagg
     3901 tattactttc gctgcgacta atcctgcaac atgtgccata tctaccataa agtaagctcc
     3961 tacttctttt gcaacctttg atattctttc ataatctatt aatcttggat atgaacttgc
     4021 ccctgcaatt ataagttttg gtctacattc ttttgccttt gcttcaagag catcataatc
     4081 tatacgtcca gtatttgggt ctaccccata aaattcatat ttataaatgt catgtaaaaa
     4141 attaactgcg ctaccatgtg tcaagtgtcc accttggtca agacgcatac ttaaaacagt
     4201 gtcatttggt tttaatattg atgaatatac acaatagtta gctgtagaac ctgaatacgg
     4261 ttgtacgttt acatgttctg ctccaaatac ttcttttgca cgttttattg ctagggtttc
     4321 aagtttatct gcttcttcag aacctgcttg aaaacgagcc cctggatagc cttcttctgt
     4381 tttatttgta aatacacatc cacttaattc aagtacttct gttggtgctg tactttcaga
     4441 agctatcatc tcaatattgt gttcttgtct aaccaactca tcagctacaa tcttgtataa
     4501 ctctgggtct gaaatttttg ttgtttttaa cataataatc ctcctcttaa ttaaagaata
     4561 tattttatta gtttaatctt cattgataaa tttattataa tggtctataa aacgtaagat
     4621 attcctccac caggtcctaa tggtatgcca atacccatga atagtaacaa tattattatt
     4681 tctattaatg taaataccac tgagtatgga atcatattcg atatgacagt acctatacct
     4741 atatctttat catatcttct ggcaaaggcc aatataactg gaaaatacgg aaaaagtgga
     4801 cttattggat ttgttgaagc atctccaata cgataagcta tttgagttaa tgctggatta
     4861 aagtctagta gcataaacat gggaacaaat ataggtgcta aaatcgccca ttttgcagaa
     4921 gcacttccta caaataaatt gataaatcca gatagcaata taaatcctat tatcaatcca
     4981 tagctaggta tccccgactc tgctagtaat tttgctcctg ttattgataa aattgttcct
     5041 aagttactaa gattaaataa attaataaac tgggatgcta taaatgctag gactatatat
     5101 ccacccatat cactcatact actacccatc atagatacta aatctttgtc actttttatc
     5161 ttcttactta cttttccata cactaatcct ggagttaaaa atataatagt gattatagga
     5221 actatacctt tcattaaagg tgcattacta gatagtatag aaccagtctc tggatctttc
     5281 ataaatgcat tgtcacctat acataaggca actatcacta ctactacaac taacaatgat
     5341 aataaagcgt attttatacc cttattttca agttcactca attcctgtat atccttatca
     5401 tttgttgaat cattatattt tgtaaaacga ggagctataa atttttcagt tacaaatgct
     5461 cctgcaaaag ttaaaactat cgctgatata aaagcaaaat aaagattcat tgctggattg
     5521 ccttggtaag atgggtccat catttgagct gctggtattg taaatccagc taacaataca
     5581 tctgtagtac taagcataac atttgctgca aatccccctg caacacctgc aaaagctgca
     5641 aacataccta caagaggatg tcttccaatt cctaaaaaca caagtgctgc aagtggtggt
     5701 aatattataa atcctacatc tgctgctgta tgtgataaca tacctgcaaa tacgattgct
     5761 acagttacaa gtttttgtgg aactttactt ataccctttt tcattaacac ttccataaat
     5821 ccagatttct cagcaacacc tgctccaagc attgtaacca gtactaatcc taaaggagca
     5881 aatgactgga agtttgtaac tatgctacct aaatatgatt gaatctgctc tttctctagt
     5941 agatttacta cctttacaac ttcttttgta gaaggatgta tttcttctac tcctaaatta
     6001 gatataactg ctgacaatat tacaacaatt atacatagta ataaaaatat ggttactggg
     6061 tctggaagtt tatttcctgc tctctctaca acatttagtg ccctcatcaa taatccacta
     6121 tctttgtttt tttcctgtga attaattttc ctcaaactaa taccccctca taaactttat
     6181 ctccagaatg ctctatctta aattttattt tttaatttat ccatataatt tataacagac
     6241 tgacatagta catttgtacc taccaatata gaatttttat cagctttaaa tttaggatta
     6301 tgccactgat atatacaatc ttgttcttta ttgcctaccc ctaaccagta aaaaaatcct
     6361 ggggcctttt ccataaatat agaaaaatct tctcctcctg tagaaggtat tggatcaact
     6421 atagcatctt ctgaatacaa atcacacaca gacttacaag ctattgtgta catctcttta
     6481 ctatttataa cagctggtaa atcaaataaa taatctagct ttgcagttgc ttgatatcca
     6541 cgagctatct cacaaactat attttctaaa ttttctgata ttttctttcg tacttcatta
     6601 ctaaagcttc tacatgtacc tgtcatatct accttttctg aaattacatt atttgcagtc
     6661 cctccattta tagtacctat tgatataaca gctgattcta atggacttat gtttctgctt
     6721 actatggttt gaatacccat tataattgca cttgatgcta ctatagggtc tattgttctg
     6781 tttggtatac ctccgtgacc tccaaatcca tttacttcta tcttaatagt gtctactgct
     6841 gccattaacc cacctaactt aacacctatt tttccacaag gtatatctgg atgattatga
     6901 agtccaaata tagcatcaac ttttggattt tccattactc ctcgttccac tagcatcttc
     6961 gcacctttat tgacttcttc ggctggttga aatataaact ttacatttcc atgaagacta
     7021 tctctaatat gagacaatac atatgcacat cctacaaggc aagatgtgtg tatatcatgc
     7081 ccacatgcat gcattatacc atcatatctg gatttatact caatatcaac ctcttcatat
     7141 attggcaatg catcaatatc tcctctcagt gctactgtgg gaccatcata cttccctttt
     7201 aataatccaa caactcctgt ttccattcct aaatctaata tttctatgtc taaacttact
     7261 aataaatctt ttatagcttt tgtagtccta aattctttaa aacttaattc ggggtgcata
     7321 tgaatatctg taaaaaaatc taaaatatct tctttatact tttctgagat taaaataagt
     7381 tcatgcatac taacacctct ccaaaatttt atttttttat tttttataat ttaactacaa
     7441 attatataag ttaataatct ttataaagat attttttata aatttaattt tagtttatat
     7501 ttatatttta ctatattatt ttaattaaat atattatcat gttttgcaat ttatttaact
     7561 attttgactt tttaaattcg gattatataa aaaatttctt taaataaaaa ctattaatag
     7621 gtgactctat tcctgacttt gtgttgattt ttttctacat gtaattgctt tatacaccat
     7681 acagactaaa attaaaagcc ccatataacc tgtatatgga tataaaatgt ttactaattt
     7741 accaaacggt aaaaaaccac ctatacaagc caaaatagct attactgttg ttaccaattt
     7801 aaatttagga tgttcatctt caacaattct attggttaca gaccataaaa gtggaacagc
     7861 tgttgtatag atacctaata ttaatgcaac tgaaaaacat acacctacaa taggagatat
     7921 attatctgct aaataaagtg atggtatttc tttcatatac aaatttccta tatctgaaat
     7981 catagccaaa tacataacta taccagcagt cataaacact attccaccta ataaacctcc
     8041 ccaaaaagca tcctttttac tttcaataga agctcccatt cctgttaaaa aaacaatcat
     8101 tacaataata tttaatgtat tatatattac accagaaacg attggacttt ctgatgcttt
     8161 tgtaacttca atatttttca tagcttctcc aaccatagat aaaccatcaa aatgcttaaa
     8221 caagcttatt aatccaacag caattgtaaa tactgctatc actggtccaa tacgtccaag
     8281 tatgcttact aatttttcta aacccataga cactgtaatt aaagtagcaa cagccatcaa
     8341 tactcttcct aagtaaggat ttataccata gtattcagtt aaagttgagc ctgcacctga
     8401 aatcattact ataagaatcc caaataaaaa tattggacta aaccattcgt aaaattttcc
     8461 taaaatcttc ccacaataaa aagtgtaaat tcttgttggt tctttaagtt ttaattctct
     8521 acctctagtt ataacttcag caccaagaaa tgaaaacaat accattgata ttaatgctcc
     8581 aattaaaccc atataccctt gtgaggcaaa aaattgtaga atttcttgac cagtagcaaa
     8641 cccagaacca attgctgttg ctagataagc gcctgcaata cagataatat ttttcatatt
     8701 cactttgtct gacatattaa attacctcca ttataaagtt accttccttt aagaaatata
     8761 aactctcagt gaattaaatg aaaattctgt ctttttgaaa cagtatttaa ttcacttttt
     8821 ttattttgta tatctttttc tttttattta tatatatttt tttatataaa taggtacagc
     8881 ttttataagg tttaatatta ttaaatatgt ttaaagcgct ttaattttta tcataattaa
     8941 agtatactta ttcagacaca ctcaaacaat tatctatatt gactttttat ttatcaattt
     9001 caatttttag tgttcagttt ataaactttt ggagtcattc ctttttctat cttaaagttt
     9061 ttataaaagt
//
LOCUS       DAEPLF010000140         8947 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEPLF010000140 DAEPLF010000000
VERSION     DAEPLF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.140"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..495
                     /locus_tag="KR314_002642"
     CDS             <1..495
                     /locus_tag="KR314_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329013.1"
                     /translation="EIVNKDELEIIENKEKEERINYCENYYFDFFKYLIKYGLEKGQF
                     TLEEFLKNYLKYDKVIYLEITKDSKLMRDIIIYFANQRESINIKKIIRMLENSAFSPN
                     TDFQIERLIERLLVEDFELLNSVSHIKSNKIFEDEMKINTEINLETMTAVKLEIANIK
                     FEII"
     gene            513..1241
                     /locus_tag="KR314_002643"
     CDS             513..1241
                     /locus_tag="KR314_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329014.1"
                     /translation="MNISLNDYKIANEIFYRLLKKSELKEKEAKELFLAYINNSNVRY
                     ALEAICEVSKIKIILISDTLYLIPNNDNDIFGFDYRRESLLGKDLKDSYLGYLIMTII
                     FSEFTNGVAPASYIQVLNLLDLVQESLERSIARDNLEEIEKSCEFNITILKNYWESKR
                     KWDESSKKGIETTSADFQIGFIRKIINFLKNEGLIVHIEDEDKIKPTRRFLDLMNNYF
                     LDEERKVLIEKLVLRKGESINASN"
     gene            1228..5583
                     /locus_tag="KR314_002644"
     CDS             1228..5583
                     /locus_tag="KR314_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329015.1"
                     /translation="MPVINKYKIINFKFGMGQERILSNRTMELYGENLQVEAENTAGK
                     TMSIQTIIQSICPLSDVAKPFTAIYNKKEPLYSMTEWLLDDGKTLLLTGIGFEKKAGS
                     SKEEGKEKKDDLYKYFMFIIEESKEMEVDIDNIPLLKNNDKGIRIVQPLTASQDYIKM
                     LRDKYGKNKVHFFNNYLRKQHREKLEEYGINQSEWKEIIIKLNSNNKEGGLSDFVKEH
                     NTSEKLIRDKILPLIETSLTTEEFAPILQIKEQVRKCINNIIDMNEKLSDYKNYRDLN
                     IRVESLQNKINRILFIEEDKNNVLGELANLYVHIENKRNELNEEIKQYQKMKASKEEE
                     FKIVEYEEDSYEYFRKEKEIQDTQVEFDKAKNELNDKIEELELDEQYLNISKFKRKKK
                     GLEEGEKDKIEKESEKQRKEVNQEEITAIINRLGSSIHYCLKEESIINNEKLKENTNN
                     NIAIQEKKNQLNDDKSKLTKVLISKNSEIKYIEGKERDFNNQLHEFKLNNTDIKGFIS
                     NNFLGEVIDYTRYINYLNIKVKSIEENLESKVMQIEESNKLKDDINEAINNLKLDNHE
                     SYMKLQVLKTRKKDIDNFKEKYKIIKTRFEIDNELDNSTKLYKEVKQELNKMKLQVQD
                     LENNKKQLEKELEDLQNYKDIIFEEEILTDLERNGIKPIEGMKYLLTLDGDLEYKNNL
                     ISKNPLLPYSIIITNKEYQKLKQINLKNKTKISMPIFVRDNIEDTFITTSSNLVISNG
                     LNILSSFDNKALDPEKREKDMNLIKEQINSIRDKVEQYKAEIEILEEFKLLTNKIDFT
                     AEDLSIDVKIENYKKIIFEINNKIKSNENNEKNLSNKIKELYENKEKLLNNKNILFNK
                     LKDIRNIQDTYSIILENKNEITKLKTSIEKIEEEKKVIKKSIEDIDVNIFNLNSEKDR
                     INRHIDDTESKINKFKIYEPIKLENAKLEELEIKYKKYLEDPTKKEIETLNYEISVCL
                     KRIESITIELEEFRQLISSDNFENIPILFTIEELNKRIVLNKSKVDELRKLKYSIKSD
                     IDKNQGSLNTKREEILKKFNGKEPLSTSLIVNFNFEERKIDIKKDIKDIGEIIEGNRT
                     TCQNLEQELRDLKKYKVERKAITYQPEDIFIESKKLQSKLQNVEYNLIKSINSYKNTI
                     LEIKEFNYKHMEKYKNFIETLNVNKDNYSRQKEQVEIVLLVIREEISKLEKHSEQLRK
                     EKTIISRQIKDYVMDCIDEFKIINKLGKHKGQALFNISLPKSDKIENSLSLVDMLMDE
                     IVEEANLGDVEQKINTFYILNKTLNIGRIPVSVIKYEINRNETIKWNSINNETTGGQR
                     FCISFIVTILLMEYKRYDRNAIVDESKYKGKVLLMDNPFGETSQQDFLKEIFELAKKF
                     NVQIISYTHVTNASIRAMFNKIYLMTVEKTTSNKEFVDINEIKQNKTDELVYMSKFYI
                     GNKEELIQEDIFSLI"
     gene            5685..5894
                     /locus_tag="KR314_002645"
     CDS             5685..5894
                     /locus_tag="KR314_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329016.1"
                     /translation="MKNFVIVSTKAALLRVIIVRLLLKIGLKTYKYLSYFMKKEKLKR
                     IIRPEGCELRVNRSIQVERAIAEQI"
     gene            6290..6430
                     /locus_tag="KR314_002646"
     CDS             6290..6430
                     /locus_tag="KR314_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0329017.1"
                     /translation="MKIKIKKYREKLLMIQNELAKSVGVRRETIVHLENGLMYHKGIQ
                     DK"
     gene            6568..7530
                     /locus_tag="KR314_002647"
     CDS             6568..7530
                     /locus_tag="KR314_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBH0329018.1"
                     /translation="MAKIDNMMSILWMLNSDKKVTAKQISEKLEINIRTVYRHIDALS
                     ASGVPIISDTGHNGGYTLLNNFIKAPLFFDTEEQTALLHAAIFAKEEGSFLDDTLDKA
                     TSKLKMYSNQKQEETLTKHLIGFEVIKPTGKISIESVLKKLKNSIANELSVEINYRTA
                     HEEKSKCRIINPYGIVRWNNNWYIVAFCQMRNEIRSFRVDRISSVIETSNTFNRPKEF
                     SASEFFIRGIMDETKDEDNLISLVIEGKMNALDNLCQHWLLGYYLKERTSNKAIFKLQ
                     EEIIFTYVPNILIPYRKSIQIIEPLSLRDKMVEDLLELINHYQI"
     gene            7608..8237
                     /locus_tag="KR314_002648"
     CDS             7608..8237
                     /locus_tag="KR314_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBH0329019.1"
                     /translation="MKKRKVYMYVFDTMSDWEVGYLTAELNTGRYFKKGLKPLKVITV
                     GVDKNPIITMGGLKVLPEITIDELNIESNDLLILPGGNTWTSIGHEKILEKSKEALEQ
                     RSIVAAICGATLGLAKTGLLDFRNHTSNDLEFLKMVILSYSGEKYYKMESAVNDENLI
                     TASGIAPLEFTVQVLKVLDVFKEEALSSWLNLYKTHDSKYFFELMNCNK"
BASE COUNT         4023 a          864 c         1375 g         2685 t
ORIGIN      
        1 gaaatagtta ataaagacga gttagaaatt attgaaaata aagagaagga ggaacgtata
       61 aattattgtg aaaattacta ttttgatttt tttaaatact taattaaata tggacttgaa
      121 aaaggacaat ttacattaga agaatttcta aaaaattatc tgaaatatga caaagttatt
      181 tacttagaaa taactaaaga ctccaaattg atgagagata taataattta cttcgctaat
      241 caaagggaaa gtatcaatat caaaaagata atcagaatgt tagaaaatag tgccttctca
      301 ccaaatacag attttcaaat tgaaagatta atagaacgtt tattagtaga agattttgaa
      361 ctattaaata gcgttagcca tattaaaagt aataaaatat ttgaagatga aatgaaaata
      421 aatactgaaa taaatttaga aactatgact gcagttaagc tagaaatagc aaatataaaa
      481 tttgaaataa tctaaatata aggggaaata caatgaatat atctttaaat gactataaaa
      541 tagccaatga aatattttat agattactta aaaaaagtga gttaaaagaa aaagaagcta
      601 aagaattatt tttagcttat ataaataaca gtaatgtaag gtatgcttta gaggcaattt
      661 gtgaagtatc taaaataaaa ataatattaa tttcagatac cttatactta ataccaaata
      721 atgacaatga tatttttggg tttgactata gaagagaatc tttattaggg aaagatttaa
      781 aagatagtta tttaggatat ttgataatga caataatatt ttctgaattt acaaatgggg
      841 tagcaccagc atcatatatt caagtattaa acttactgga tttagtacaa gaatccttag
      901 aacgttcaat agctcgtgat aatctagaag aaatagaaaa atcatgtgag tttaatataa
      961 ctatcttaaa gaactattgg gaaagtaaaa gaaagtggga cgaatctagt aagaaaggaa
     1021 tagaaacaac atcagcagat ttccaaatag ggtttattag aaaaataatt aatttcttaa
     1081 aaaatgaagg cttaattgta cacattgagg atgaagataa gataaaacca actagaagat
     1141 ttcttgatct tatgaataat tattttttag atgaagaaag gaaagtatta atagaaaaac
     1201 tagttttaag aaaaggggaa agtattaatg ccagtaatta ataaatacaa aattataaat
     1261 tttaaatttg gtatgggaca agaaagaata cttagcaaca gaaccatgga actgtatgga
     1321 gaaaacttac aagtagaagc agaaaataca gcaggcaaaa ctatgagtat tcaaactatc
     1381 atacaaagta tttgtccact atcggatgta gccaaaccgt ttactgcaat atataataaa
     1441 aaagaaccat tatactcaat gactgaatgg ttgctagatg atggtaaaac gttattatta
     1501 acgggaatag gatttgagaa aaaggctgga tcatctaaag aagaagggaa agaaaaaaag
     1561 gacgatttat acaaatactt tatgtttatt atagaagaat ctaaagaaat ggaggttgat
     1621 atagataata tacctttatt aaaaaacaat gacaaaggta taaggatagt acaaccttta
     1681 acggcctcac aagactatat aaaaatgcta agagataaat atggaaaaaa caaagtacac
     1741 ttctttaata attacttaag aaaacaacat agagaaaaat tagaagaata tggaattaac
     1801 caaagtgaat ggaaagaaat tattataaaa cttaatagta ataacaaaga aggtggacta
     1861 tcagactttg ttaaagaaca taatacttca gaaaaactta taagagataa aatattacct
     1921 ttaatagaga caagcttaac aacagaagaa ttcgctccaa tattgcaaat taaggaacaa
     1981 gtaagaaagt gtataaataa tattatagac atgaatgaaa aattatcaga ttataaaaac
     2041 tatagagatt taaacattag agttgaaagt ttacaaaata aaataaatag gatcctattc
     2101 atagaagaag ataaaaataa tgtattagga gaattagcta atctatatgt acatatagaa
     2161 aataaacgca atgaattaaa tgaagaaata aaacagtatc aaaaaatgaa agcatcaaaa
     2221 gaagaggaat ttaagatagt agaatatgaa gaagatagct atgagtattt tagaaaagaa
     2281 aaagagatac aagatactca ggtagaattt gataaagcca aaaatgaatt aaatgataaa
     2341 atagaagaac tagaattaga tgaacaatat ttaaatatat caaagttcaa aagaaaaaag
     2401 aaggggctag aagaagggga aaaagataag atagaaaaag aaagtgaaaa acaaagaaaa
     2461 gaggtaaatc aagaagaaat aacggctatt ataaatcgtc taggtagcag tattcattat
     2521 tgtttaaagg aagaaagtat tataaataat gaaaaactta aagaaaatac aaacaacaat
     2581 atagctatac aagaaaaaaa gaatcaactt aatgatgaca aaagtaagct cacaaaagta
     2641 ttgataagta aaaattcaga aattaaatat atagaaggca aagaacgtga ttttaataat
     2701 caactacatg aatttaaact taataacact gatattaaag gctttatttc taataatttt
     2761 ttaggcgaag taatagatta tactaggtat ataaattatt taaatataaa agtaaaatca
     2821 atagaagaaa atcttgaatc gaaagtcatg caaatagaag aatcgaataa acttaaggat
     2881 gatataaatg aagctataaa taatcttaaa ttagataatc atgaatctta tatgaaactg
     2941 caagtattaa aaactagaaa gaaagatata gataatttta aagaaaaata taaaatcatt
     3001 aaaacaagat ttgaaataga caatgaatta gataactcta ctaaactata taaagaggta
     3061 aaacaagagc ttaacaaaat gaaacttcaa gtacaggact tagaaaataa taaaaagcaa
     3121 ttagaaaaag agctagaaga tttacaaaat tataaagata taattttcga agaagaaata
     3181 ttaacagatc tagaaagaaa tggaataaaa cctattgaag gcatgaaata tctattaacc
     3241 ttagatggag atttagaata caaaaataat ctcatatcta aaaatccatt gctaccatat
     3301 tctataataa ttacaaataa agagtatcaa aaactgaagc aaataaactt aaaaaataaa
     3361 actaaaatct caatgcctat atttgtaaga gataacatag aagatacttt tataactaca
     3421 tctagtaacc ttgtaattag taatggatta aatatactat cttctttcga taacaaagct
     3481 ttagatcctg aaaaaagaga aaaggatatg aatctaatca aagaacaaat aaatagtatt
     3541 cgagataaag tggagcaata taaagcagaa attgaaatat tagaagaatt taaacttcta
     3601 actaataaaa ttgattttac agctgaagac ttatccatag atgtgaaaat tgaaaattat
     3661 aaaaaaatta tatttgaaat aaacaataaa attaagagta atgaaaataa tgagaagaat
     3721 ttatctaata aaattaaaga actttacgaa aataaagaaa agctattaaa taacaagaat
     3781 atattattta ataaattgaa ggacattaga aatatacaag atacatattc tataatttta
     3841 gaaaataaaa atgaaataac taagttaaaa acaagtattg aaaaaatcga agaagaaaaa
     3901 aaagttatta aaaaatcaat agaagatatt gatgttaata tatttaattt aaattcagaa
     3961 aaagatagga taaataggca tatagatgat acagaaagca agataaataa atttaagatt
     4021 tatgaaccta ttaaacttga aaatgctaaa ctagaagaat tagaaataaa atacaagaaa
     4081 tacttagaag accctacaaa aaaagagatt gaaacattaa attatgaaat ctcagtttgc
     4141 ttaaaaagaa tagagagtat tactatagag ttagaagaat ttagacaact tattagtagt
     4201 gataattttg aaaatatacc tatattattc acaatagaag aacttaataa aagaatagtt
     4261 ttaaataaaa gtaaggttga tgaactacga aaacttaaat atagtattaa aagcgacata
     4321 gataaaaatc aaggttcatt aaacactaag agggaggaga tacttaaaaa atttaatgga
     4381 aaagaacctt tatctactag cttaatagtg aactttaatt ttgaagaaag aaaaatagat
     4441 ataaaaaaag atataaagga tattggtgaa attatagaag gtaatagaac tacttgtcaa
     4501 aatctagaac aggaattaag agacttaaag aaatataaag tagaaagaaa agctataacc
     4561 tatcaacctg aagatatttt tattgaaagt aaaaaactac aatcaaaact gcaaaatgtt
     4621 gaatataatt taattaaatc tataaatagc tataaaaata caatattaga aataaaagaa
     4681 tttaattata agcacatgga aaaatacaaa aactttatag aaactttaaa tgtaaataaa
     4741 gataattact ctcgacaaaa agaacaagta gaaatagtat tactagttat aagagaagaa
     4801 ataagcaaat tagaaaaaca tagcgaacaa ctaagaaaag aaaagactat aatatcaagg
     4861 caaataaaag attatgttat ggactgtatt gatgaattta aaatcataaa taaacttggt
     4921 aaacataaag gacaagcctt attcaacata tcactaccta aatctgataa aatagaaaat
     4981 agtttgagct tagtagatat gctaatggat gaaattgtag aagaagcaaa cctaggtgat
     5041 gttgaacaaa aaataaatac cttttatatt ttaaataaga ctcttaatat agggagaatt
     5101 cctgtatcag ttataaaata tgaaataaat agaaatgaaa ctataaagtg gaactctatt
     5161 aataacgaaa caacaggagg acagagattt tgtatctcat ttattgtgac aatattgttg
     5221 atggaatata aaagatatga tagaaatgca atagttgatg aaagtaaata taagggtaag
     5281 gtactactta tggataatcc ttttggagaa acgtcgcagc aagatttcct taaagaaata
     5341 tttgaacttg ctaaaaaatt caacgttcaa attatatcct atactcatgt aacaaatgct
     5401 tctataagag caatgtttaa taagatttat ttaatgacag ttgaaaaaac tacttctaat
     5461 aaagaatttg tggatattaa tgagattaag caaaataaaa ctgatgaatt ggtgtatatg
     5521 agtaaatttt atataggtaa taaagaagaa ttaattcaag aggatatatt tagtttaata
     5581 taaataacaa gtaaacaaat aaagtgatta ctatcagtga ggcaggtaga aactgaatta
     5641 ctttgtgact cattacgact cttggggtaa aattgtgtat aaatgtgaag aatttcgtga
     5701 ttgtcagtac aaaagcagct ttattaaggg taataatagt aagactcctc ttgaaaatag
     5761 gactaaaaac ttacaagtat ctaagttatt tcatgaaaaa agaaaaatta aagagaatta
     5821 taagaccaga gggatgtgaa cttagagtga atagaagtat acaagtagaa agagctatcg
     5881 cagaacaaat ttaattttat catacgatag cctcttgtta ttttagtgat gaaatccatc
     5941 aagcatacca taactacaga aaaagaggct tttctgatgg tatattgggt tcttttagag
     6001 tgtgtgattt gctacatgtt cattaggata ataatagtca gtaaatgata ttactgatag
     6061 aatagatata ataaactaat ctagttccat attgtaatgt ttattaaaga gtatactttt
     6121 atgattataa tagctatttt ctttgaagct tattgtacaa gagatttgaa tatatcaaat
     6181 ttggatatag gtataattct cttagcagtt ttatttatac agtctttatc taaattagtg
     6241 ttgtatatac tttgataaaa gggggatatg taactaagtt gtaatattga tgaaaataaa
     6301 aattaaaaaa tatagagaaa aactccttat gatacaaaat gaacttgcta aatcagttgg
     6361 ggtaagacga gagactattg tacacttaga aaatggatta atgtaccata aaggtatcca
     6421 agataaatag ttaaaaaact agataagaat aaggagcctt tacagaacaa atttagtgtg
     6481 ctcgtgcgat agcccttttt ttataaaaat taatctaata acctatataa ttaaatttat
     6541 aaaacatata tgaaagggta aaactatatg gctaaaattg ataatatgat gtccatatta
     6601 tggatgttaa actcagacaa aaaagttacc gcaaaacaaa tatctgaaaa actagaaata
     6661 aatataagaa ctgtctacag acacattgat gcattatctg ctagtggagt acctataatt
     6721 tcagatacag gtcataatgg tggatataca cttttgaata atttcatcaa agcacctctt
     6781 tttttcgata cagaagaaca aactgcgtta cttcatgcag ctatatttgc taaagaagag
     6841 ggaagtttct tagatgacac actagacaaa gcaacctcaa agttaaaaat gtattcaaat
     6901 caaaaacaag aagaaacttt aacaaaacat ttaataggtt ttgaagtaat aaaaccaact
     6961 ggaaaaatat ccatagaatc tgtacttaaa aagttaaaaa atagtatagc aaatgaatta
     7021 tctgtagaaa tcaactatcg tacagctcat gaagaaaaat caaaatgtag gattataaat
     7081 ccatatggaa ttgtacgttg gaataataat tggtacatag tagcattttg tcaaatgaga
     7141 aatgagattc gtagttttag ggtagataga attagtagtg tcatagaaac atcaaatact
     7201 tttaataggc caaaagaatt ttcagcaagt gaatttttta taagagggat tatggatgaa
     7261 acaaaagatg aggataatct tatatcatta gttatagaag gaaagatgaa tgcattggat
     7321 aacttatgtc agcattggct tctaggatat tatttaaaag aaaggacttc aaataaagca
     7381 atatttaaac ttcaagaaga gataatattt acatatgtac cgaatatact tataccttat
     7441 agaaaatcta tacaaataat tgaaccatta agccttaggg ataaaatggt tgaagattta
     7501 ctcgaattaa ttaatcatta tcaaatttaa taacttaact gacagagtat gtcagttaag
     7561 ttattttaca attaggtata ttaattaatt atagaaggag caaaaaaatg aaaaagagaa
     7621 aagtatatat gtatgtattc gatactatgt cagattggga agttggatat ctaactgctg
     7681 aattaaatac aggaagatat tttaaaaaag gattaaaacc actaaaagta attacagtag
     7741 gagttgataa aaatcctatt ataacaatgg gtggattaaa agttttacct gagattacaa
     7801 ttgacgagct taatattgaa agtaatgact tattaatttt gccaggggga aatacttgga
     7861 catctatagg tcatgagaaa atattagaaa aatctaaaga ggctttggaa caaaggtcta
     7921 tagttgcagc tatttgtggt gctactttag ggttagctaa aacaggttta ctagatttta
     7981 gaaatcatac aagtaatgat ttagaatttt taaaaatggt gatactaagt tattctggag
     8041 aaaaatatta taaaatggaa tctgcggtta atgatgaaaa tttaattact gcttctggaa
     8101 ttgcaccatt agaatttaca gtacaagttt taaaagtttt agatgttttt aaagaagaag
     8161 cattaagttc ttggttgaac ctttataaga cgcatgactc taaatatttt ttcgaactaa
     8221 tgaattgtaa taaataaatt taagatttaa ttttaaattc aaattttaaa ttactttttc
     8281 tatggcaata gataagtagt ttagaatttt atcatatata taatttgtat ttataccaac
     8341 acaaatagaa atgatagttc caataataaa atttataatt agttccatat ggagtacatt
     8401 tctttcctac taatataact aagaatatat gaaaccacca tagattgtag ttttttctgt
     8461 tgtattatat catgggatag taaagtgacg tatatttttg atgaaaacct aggattgtat
     8521 atgaaatgaa ttaatcctgt gtattaatct tgagattcgt gtctaatgat ttgtaaaaat
     8581 aaataatgaa atacttagaa atacttgtaa atgctagtca atattggcta gtatttttta
     8641 ttttagtaat gaaatcccca actgtacttc atccacaaaa aaagagatat ttttgagggt
     8701 gtattggagt gaacttaggc tgtataatta tacatttcaa tatcttatat taattaggat
     8761 aatagttggt aaatgatatg attgaaacaa tagatataat aaactaatct agtactgtac
     8821 tgcttactaa agaatatatt tttatggtat aagaattttt tagagcgttg attctatagg
     8881 agtgaataaa aaagtaattt tataatatta tttgtaacca ttagatgtct attatgaaat
     8941 cttttat
//
LOCUS       DAEPLF010000141         8917 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEPLF010000141 DAEPLF010000000
VERSION     DAEPLF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.141"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            122..505
                     /locus_tag="KR314_002649"
     CDS             122..505
                     /locus_tag="KR314_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329020.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKVVLKSPEYIGSHPKKPGKSVEFIKKIDNYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNNGRVKQYDG"
     gene            1011..1394
                     /locus_tag="KR314_002650"
     CDS             1011..1394
                     /locus_tag="KR314_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBH0329021.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            1396..1746
                     /locus_tag="KR314_002651"
     CDS             1396..1746
                     /locus_tag="KR314_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329022.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(1840..2298)
                     /locus_tag="KR314_002652"
     CDS             complement(1840..2298)
                     /locus_tag="KR314_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329023.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(2343..3131)
                     /locus_tag="KR314_002653"
     CDS             complement(2343..3131)
                     /locus_tag="KR314_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0329024.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(3270..3707)
                     /locus_tag="KR314_002654"
     CDS             complement(3270..3707)
                     /locus_tag="KR314_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBH0329025.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            4529..5038
                     /locus_tag="KR314_002655"
     CDS             4529..5038
                     /locus_tag="KR314_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329026.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVIVIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            complement(5949..6749)
                     /locus_tag="KR314_002656"
     CDS             complement(5949..6749)
                     /locus_tag="KR314_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329027.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(6944..8542)
                     /locus_tag="KR314_002657"
     CDS             complement(6944..8542)
                     /locus_tag="KR314_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0329028.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIIW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
BASE COUNT         3232 a         1312 c         1121 g         3252 t
ORIGIN      
        1 aaaaaactat attatactat cttttctttt tggaataaac tatactttaa atatagatat
       61 ttaatttaaa taaattttac agtattaata gtaaattatc ttagaaagga atgtgcttta
      121 tatgtctaca gacaaagttg ttggaatcat agatgaagaa acggcagaac ttgcaggaat
      181 tgaatatact ggtaaaattt atgcttcttc tggtgttata aaacatataa agaaaaaaca
      241 tagatgtcaa ctgagtaagg atatttttaa tgatataata gacactataa aagtggtatt
      301 aaagtcacct gaatatatag gaagtcatcc taaaaaacct ggaaaaagtg tggagtttat
      361 aaaaaaaatt gataattata ttttagtagc tacagaatta gatacaaaaa aaggatattt
      421 atatgtatct tcaatgtatg ctataaaaga agcaaaatta gaaagtagag taaacaatgg
      481 aagagtaaaa caatatgatg gttaacataa tattattaat tttccccaaa atattgaaaa
      541 atattattat ataagttata atattaatat acacgtagat atttattagt tagatttgag
      601 gttggaaaag gcacccaata cgctactata gagtagttaa tagagatgca ggatacgccg
      661 ccctgccaaa tctaattaaa ttaaaaagag aggattaatt cctctctttt tgtttttttg
      721 agatactaat ttaatctata tagaaagatt gaaggtcatt tatactaaaa gttttcagtt
      781 atatacatta aactaattaa ataagatatt tataatctat atatgataca tattatatat
      841 taataaaaga aaaatatata gaaaatcctt ataaaaattt aaattgtaat gaatagtaag
      901 taattaaaaa gaatttttat tgatttttaa aaatttagtt agaaactata tcattatggt
      961 aaagaatgta ttataatgtt tagaaatatt ataataagga gcgattaaac atgaatctat
     1021 cgaagaaaat agaagataaa agaaaagcta aacagagagc tgaaaaagtt aagaaagcta
     1081 aaattgctac agctggtgtg gtattaggag ctgttactgg tgcagtgagt ggagtacttt
     1141 tggctcctaa atctggaaaa gaaacaagag aggatataaa agatgcttca caacaaattg
     1201 ctgaaaagat aaatatgaaa actgtagatg taaaaggcaa agtatctgag aagctagagg
     1261 ataaaaaagg aaactttata gaatcaaaga aaaaaataaa aaaatatata gataacaaaa
     1321 agagtgttga aaattctagt gaggaagata aaataataga tatagttgaa ccagttagtg
     1381 ttactgagga ataatatgga caatacaata acgattaaca taagtataat atatgtatta
     1441 ttagctatac ttgcttgtat tgctttagtt tatttaatta ttgtacttgt taaactacat
     1501 aagattttaa ataatgttgg aactatgtta aatgaaaata ctcaaaatat aaatacaact
     1561 ttaaataatc ttcctaatgt tgtagaaact tttggagaag taggagaaaa tatgaaagat
     1621 gtaacagaag tagttactga agttacagca gattttcttg ttactaaaga cagtgtcaaa
     1681 tcaaatatgg atgttatagt cgatatattg actataatta aaaatatttt tctagataaa
     1741 aagtaataaa tttaatataa aataagatat aatttaaacg accttaaaat aacatttata
     1801 aggtcgtttt ttattagctt gatttgacac tatgtgtttt taataattgg taatattcct
     1861 ctttatcatt tgaacatata tatctatgct tgttgtttca attacatcca taagaacatc
     1921 tttacattca agctcaaaat cttccattcc ttctaaatat tcatcatctt catcaaaaaa
     1981 gatataagaa ttatatagcc attcttcaaa tttttcttca agtatatccc aatacgaact
     2041 ccaaaataat ccacaacctg atatataaga tgctctactt tgatttatat aaacctcatt
     2101 tagaagcttt tgtaatctac tgctataaag aaaatctgct cttccactgc ttgatattaa
     2161 ttcatttaaa tcttctattg acattaattc aactttacca cttttatcat aaggagtata
     2221 attttcatca acccatttat gtggtttgtt ttctctgcat actgttgcta tcaactctac
     2281 aacttttgtt cttatcattt ttttatccta acttatttat tttttataaa ataatcatga
     2341 ttttataatt tatctatttc ttgtaatgct aatttaacag tttcatatct atcagcacca
     2401 tttattacta catcaccttt tacaagttta ttagcttctc cacctacaac ataaaccttt
     2461 ttacctaaac cttgcttata actagacgcc tcaattatta aagaatcttt caattgccaa
     2521 tgtaaaatct gagcagccac tttatcagca tcacctgtat aaacgattac attttcatac
     2581 actttatttt cccctccccc ttgagaattt gatatagatt tgtttagtat accttctgct
     2641 atcaatttag caactttatc tttattatta acataataat ctgtatctgc tttactatct
     2701 acaaaacata cttctattaa tattgctggt gcaatagtat gattaagcca ataaagacct
     2761 ctaacatcag actttgcacc cctatctttg aatactgtag ctaacctagt attcactctt
     2821 tctgcatagg ttttgccatt attagtctta tatattgttt ctgtccctac tggagttgaa
     2881 gttgtagcat ttgaattaaa atgaatctga acagccaaat cagtttcctg tttatttgct
     2941 atagcacatt ggtctgctaa atgattactt gattcatcaa ctcttcctgt ataaacagta
     3001 gctccaccta ttttaagcca tttaacaatt aagtcattca gtattcttgt ttctttgctt
     3061 tcgtttatat acccaacagc tcctgtacct tttccaatta aagtgtgccc tggtattatt
     3121 actactttca aactaacatc tcctttacaa ttaaatttaa cttctattta atatatgcat
     3181 aacctagaat tttgtttctt tttatatctt atacttttat tataacccat tttgtaaaaa
     3241 tatagaatat taagtaaata atgttaaaat tataattttg tatataagac attacttgat
     3301 tgcaataatt ttatagcata ttcatctatt ctatatccag ttctataata tatatgttta
     3361 attccagctt gaattatagc cttagtacaa tttaagcaag gaaagtgagt tacataaata
     3421 gagcaatctt taactgaaat accttctttt gcacaataat aaagggcatt ttgttcagcg
     3481 tgaatggttc taatacaatg tccatctctc atttcatgac caacttcact acaatgctta
     3541 tctccagaaa tactaccatt atatccagta gacactattc tgttttctga attgacaatt
     3601 atagctccta cataagctct atcacaagtt cctctttcag ctactgtttc gcacagtctc
     3661 ataaaatatt cttgccatga gcatctattt tcaagttttt tattcaaatc atatacctcc
     3721 aaaatttata tctaatatat aagatttata ccttaaaaat aatattaaaa acaaatttat
     3781 acgataaata aaatttatta atactgtttt tagtctaaat tatttcattt taaattaaag
     3841 ttacaaggaa tctaaaatta tatatatctt tcttcgtaat atgaatgtta caatataaaa
     3901 aactgtctca ataaaataga gacagtttta cagtcaaagt ttataataca agttcatatt
     3961 tttctttaaa ttcttttaca gcttcgggcc atgccttttg ttctgtaata cctttttctt
     4021 tagctatttt aattgcaatc tctcttatca ttgcagcgcc ttctaatatt cccattttta
     4081 atctcccttc aaatatcttt ttgtatagaa tataaatagt gctaagattt attacatgct
     4141 gcatatacaa tatctataca acacataact tgtaaattcc ttttattttc atacgtcttt
     4201 aaccgacata taattatatt caataagttt taaaattatg aactcaatta taattatttt
     4261 tattttttta gaatctaaac actaaaaatt gtaggtaaat ttgtcttatg cctttataaa
     4321 tatttctaca gttttaatta ctggacatat ctatttttat tataaaaagc gcttaaaaat
     4381 cgagaatatc cgcgtaaaag ctgtatataa gcaaaacgat taaaggaaaa agaagaaatt
     4441 tacttgaatg ttaaaatgcg ttgcttgaag caacgggcag ttttgcctat aatatcggta
     4501 acaactgaaa gaaaggtggt tagccctaat gttaagcgaa aacattaaaa caatcaggaa
     4561 gtcaaaagga ctttcacaac aagaacttgc tatcaaactg aatgtagtgc gacaaacaat
     4621 atctaaatgg gaacaaggac tgtcggttcc cgattctgat atgttgattt ccatatcaga
     4681 ggtgcttgaa acgcctgtaa gcactttact tggagaaact attgttgagt cggaagttga
     4741 tagcttaaaa gtgatttctg aaaaattaga gattataaac ttgcaacttg cacaaagaaa
     4801 aaacatgaga agaaaaatgt ttcattggtt atttatttta ttatgtgcag tcatagtaat
     4861 agtttttgtg gtcttagggg gactaaatag tccttacttg gattggaatt ataccgaccc
     4921 tgaaaccgct gtttttggag tagcttttca tgcttttgag tggctatttg tcagattagt
     4981 accgattgtc cttataggag caattattgg aatctttttg acacgtaaga aaatatgaag
     5041 taactctgct ttttataata tagcaagcgt tccttaaaag tgtcattcta catggcaatc
     5101 gaggacaact atgtaaggaa gaaccttttt gatttccaac taagcgaagt tattgaggac
     5161 gccagtaggc ccaaaaccgc attaagtaaa gtacaggaag caagactgct ggacttatac
     5221 tctcatgcgt cagatgcaga aaatgcgagg tctgtagcat gatttacaac gatttgtaca
     5281 acgactacga gtagaaatat atggagataa gtattatcta cgaaatcaga aaagaaatga
     5341 cttgataatt tactcatgtt ataattattg atgtgaaatt aataaatcga aattcctttt
     5401 ttagatttaa caatactgag taaggaaaaa gtgaatgact tttgcaagga ggtgttaata
     5461 tcaggcaaat aaagtctttt gtttttccag ttatatcctt aatattctta gatcaaatta
     5521 gcaaagttct tataggatta ttcttaatgg actttgaaat tgatataatt gggaaatttt
     5581 taagattcaa tcctgttcaa aatacaaaat taagtctctg ctatctaaca aacggagcac
     5641 aaaaatgaaa attaaatatt aaatataaag cacagttaat tttgattaaa aagttgactg
     5701 tcttttcttt tttataaatt taagcatggg taataaagaa atgactgcat ttaagcatta
     5761 tgtatataat tggaggcaat aaaaagccag caaagcccat aaaatagagc tgtgctagca
     5821 acatgatgag ttataagaag ataatcaaaa gcttacgctt tattttaatt taaaaaaatg
     5881 attactgtaa agaaatcatt ttttattata atcactgata catcaaatat tctaaataaa
     5941 atttttattt atctggaatt gtatattttt tctataatat taactacagc gtacattgcc
     6001 caagcacaaa tagccaatac aaatactccc atcattacta aatctaattt aaatacttga
     6061 gaaccatata ctatcaagta gcctattcca tatcttgaaa ctaaaaattc tccaactatt
     6121 acacctaccc atgccatacc aatattaatt ttagttaaat ttattaaatt tcctgtgttt
     6181 gcaggtaata tgagtttaaa taatatttga gatttagtag ctccaaagct ttttaacatc
     6241 ttaatttttt cttcatctat attcataaaa taattataag ctgaaagtat agtaacaaca
     6301 acagaaatcg ttacagctgt tacgacgatt ccttctattc cagcacctac ccaaactata
     6361 attataggag ctaaagcagt cttaggaaga gcatttaaaa ctaccaaaaa tgggtctaat
     6421 atttttgata atttttctga ccaccaaaga gcaattgcta ccaaaatacc taatacagta
     6481 cctataacaa gaccaagaac agtttcatat acagatattc caatatgttt aaataattga
     6541 ccactaaatg catattgaat aaataaattg taaatatcac ttggtttgct aaatagaaat
     6601 gtatttataa catttaagtc agctaaaagt tcccagatta caataaaacc aattaaaatt
     6661 aatagctggt agaataatac atttcttttt tctcttttta cacctttaac atagtttata
     6721 tatccttcag atcttttatt aagcctcatg tgtatctagc tccttccata atatgtcgaa
     6781 atacttttga aaatcaggaa ctttacgtgt tttgagaggc gtttttgttc catccatgtt
     6841 aagttcaata tcgtaaatat tttttatggt agctggtctt ttagatagta ttgcaacctt
     6901 atctgatatg cttattgctt ctgctatagc taaaaaagtg caactaattg cttaaagaaa
     6961 cagaattatc cacttcagca cccatataaa caatattaag atttcctaaa ctgctatccc
     7021 aaatgatact attaataata gtagataata acaatctctt atcatctaaa ctggcattat
     7081 caacttcatc attaaatctc ttcaaattac taacaataat atcaatatta atactgtcac
     7141 tactaatatt gatattacta gattccaaaa tattaatctt atcctttaaa atactaatct
     7201 cattatttag gctctcaatc tgggaaacta tataatcaga agcagtacta ccagtatttt
     7261 ttgcaagctc tataactaaa tttttaataa gtctttcctt atctaaaatt tgatttttaa
     7321 gtaaattagt ctcagattta aaaatattaa aaacattatc agtcttaatg ttattactat
     7381 tgaatgcact tataatagac tcttctttac aattcttaat tttatctata actaaacttt
     7441 caactaatgg accattaaga tttttacagt tgcaactatc agacccaagt gtttttttag
     7501 taccacaaat ataataatac tttaaatcct tgtcagtttt tttgccttta taggtaattc
     7561 tcatattaga accacattta ccacatttta taagaccaga taaaagagca gtttttccag
     7621 ttgatatcct tggagtttta tcacgatttt tatctaattg attttgtact tcaagccata
     7681 tttcaggttc tatgactcca tggtgttttg caattgctgc tattgagtta gatgtatttt
     7741 tagcataggt aaggattcca cattttctat ctggagtacc tacaacatcc ataccactat
     7801 ttttaaggta atctaaaatt tcatcattag cttttacata tgctggattt ctaagtattc
     7861 tagataaaga actagggtca aatttaccac cacgaggacc ccttattcca ttattataga
     7921 catatttata taacttagat aaggacctaa gttctaaata tttattgtat attagcttta
     7981 caatctccat ttgttcatca tttacttgaa gcttatacat acttctttct tttagatttt
     8041 catctaagaa tgtgattttt tcactgttga atccatgagg tgtattgcct ccaagccatc
     8101 taccactacg ggctaattca tacatgttgt cttttatacg ttctgctatt gtttctcttt
     8161 ctaattgagc aaatacactt gatataaaca tcatagctct tcccattgga gttgaagtat
     8221 caaattgttc tttgatagat ataaatgata tgtttagttc atttaatttt tctattaaag
     8281 tagagaagtc agatacattt ctcgatatcc tatctaatct ataacaaact aggtaactaa
     8341 atttcttatc tttagcatcc tgcatcattt tcttaaattg tggtctatct atattgctac
     8401 cactaaatcc ctcatcttca tatatcaaaa aatcttttac accaatagac tttgcataat
     8461 caatacataa ttgtatttga ttttctatag aatcaccttt tccagtaaat cttgattttc
     8521 ttgaataaat tgcagctttc aaaattaaaa cctcctataa attaggatat atatttgaaa
     8581 ttatttatat acaacctttt aagtaaactt attagaataa atttattaat taggtaaatt
     8641 tatataattc tactttttat aatatatgac tatttatttt atttctttac tattcaagtt
     8701 ctaaatcaat aataaatttt gatatttaat tgtatcagga caaatctcta atttcatata
     8761 ttattaatat aaaatcatag gttagtgtga aaaagttgaa atttaaatat tttaaaagat
     8821 aattttttgg aactaatagg tataacaaaa atttataggg ggattgcata tggaaatcaa
     8881 tgttatagaa attttcccta aagataaagc taaactt
//
LOCUS       DAEPLF010000142         8858 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEPLF010000142 DAEPLF010000000
VERSION     DAEPLF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.142"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            107..1168
                     /locus_tag="KR314_002658"
     CDS             107..1168
                     /locus_tag="KR314_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBH0329029.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGRKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            1259..2872
                     /locus_tag="KR314_002659"
     CDS             1259..2872
                     /locus_tag="KR314_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0329030.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            3147..5132
                     /locus_tag="KR314_002660"
     CDS             3147..5132
                     /locus_tag="KR314_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBH0329031.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLSFGILEKPVVWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMIEELAKVKSFDEFYKIITKRR"
     gene            5137..5571
                     /locus_tag="KR314_002661"
     CDS             5137..5571
                     /locus_tag="KR314_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBH0329032.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            5600..6160
                     /gene="srlA"
                     /locus_tag="KR314_002662"
     CDS             5600..6160
                     /gene="srlA"
                     /locus_tag="KR314_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBH0329033.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            6224..6592
                     /locus_tag="KR314_002663"
     CDS             6224..6592
                     /locus_tag="KR314_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBH0329034.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            6617..7183
                     /locus_tag="KR314_002664"
     CDS             6617..7183
                     /locus_tag="KR314_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBH0329035.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            7224..7589
                     /locus_tag="KR314_002665"
     CDS             7224..7589
                     /locus_tag="KR314_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBH0329036.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTNDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            7690..8463
                     /gene="srlD"
                     /locus_tag="KR314_002666"
     CDS             7690..8463
                     /gene="srlD"
                     /locus_tag="KR314_002666"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBH0329037.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIETEFGCKSVGYGCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
BASE COUNT         3534 a          909 c         1642 g         2773 t
ORIGIN      
        1 ataatgtggt gaaattatac ccatatataa ataaaataaa acaaataaat agctaattaa
       61 ggagaaaata ttttttattt tttgtttagg ataaataaat tttattatgg aaaaaagaaa
      121 aagcatacat cttcacaaat tgaatattta tttaataggg ggaggtgata gattgtttat
      181 cacagtggta ttatttttag taggtttcct attaattact aaaggcgcag atatctttat
      241 aaattgcact gtagagatag gtagaaaaac gaatatatca gaaattatac ttggagctac
      301 tatagttagt tttgcaacaa cattacctga atttacagtg tctctacttg cttctattga
      361 tggtcatact actatgagtt taggaaatgc agttggttct ataatatgta atacaggatt
      421 agcattaggg ttggttgttt ttataagtcc atttaatgta gataaaaaaa tgtttttttc
      481 taagtcttta ctattaatag tatccgttat agttttaatt ttattgagtt tagatggagt
      541 tataacaaga ggtgattcat tattacttat aataatatta atcttttata tggttaataa
      601 ttatagaagt gtcgttggaa aatctgatac taaaaataga aatattaaaa ataacactat
      661 taaagacata tctactaaaa aagggaaaaa ttatagaggt ttttccgtat tagaaattgt
      721 aaaaatacta ttactttttg ctacaggatt gataatgatg ataataggtt cacaaatcct
      781 tatagagagt ggagtaataa tagctagttt tttaaatata cctcagggta tagtaagttt
      841 aactataata gcacttggaa cttctctgcc agagatagtt tcgtcgatta ctgctataag
      901 aaaaaatcat catgaaatat cagtaggaaa tattttgggt gcaaacatat tgaatatagt
      961 atcagtaata gctgtatcag caataccaaa taacatacca atactatctc aaaatagaca
     1021 attggatata ccattcatga tattattatt attaatagta ataataccaa cacttaaatc
     1081 taatagactt agtagaatac aaggtatatt gatgttattc acatattttc tgtacatatc
     1141 aattttgtat ttcatgtata ttatttaact atactgttta aaatattaat tgcagaatat
     1201 cctaaaaaag tatataatgg ataggttaag attaattgat aacggagagg aataatatat
     1261 gaatataaca aagtttgaag atttaccaat aagtgaggga ataaaaaaag ctatagctga
     1321 aatggggttt gaagaaccat ctcctataca agcacagtct atacctgcga ttttgtcagg
     1381 taaagacgtt ataggacaag ctcaaactgg tacgggaaaa acagctgctt ttagtatccc
     1441 tatactagaa acaatagacc caaataatag aagtttacaa gctgtagtgc tttgtccaac
     1501 aagagaactg gcaatacaag tatcaacaga aataagaaag ttagctaagt attcgcacgg
     1561 aataaagaca ttacctatat atggtggcca gccaatagat agacaaataa aatctctaaa
     1621 gagtggtgtg caagttgtaa taggtacacc aggacgtaca atagaccata tcaatcgtaa
     1681 aacattaaag atggataatg taaaaatgat tatattagat gaagcagatg aaatgctaga
     1741 tatgggattt agagaagata tagaaatgat tttaagtaag attccagaag aaagacaaac
     1801 aactttcttt tcagctacta tgccaagagg aattttggaa ttaactaaga gatatcaaaa
     1861 agacccagag catataaaag ttgttagaaa agaacttaca gtttcaaata caaagcaata
     1921 ctatatagaa actaggtcat caaataaatt agaagtatta tgtagattag tagatgtata
     1981 tgaccctaaa ctatctgttg ttttctgtaa tactaagaga aaagcagatg aattggtagg
     2041 agatttacaa gcaagaggat attttgcaga tgcattacat ggtgacttaa aacagactca
     2101 aagagatata gtaatggata agtttagaaa tggtacaata gatatacttg ttgctactga
     2161 tgttgcagct agaggtatag atgttgatga tgttgaatgt gtattcaact atgacttacc
     2221 acaagatgaa gagtattacg ttcatagaat tggtagaact ggtcgtgctg gtagagaagg
     2281 tatgtcattt acttttgtat ttggaaaaga aatgagaaag atgaaagata tagaaagata
     2341 tactaaatct aaactaataa agcataatat accaacaata actgatgttg aagagaaaaa
     2401 agtaggtact ttctttgcac aagttaaaca aactatagaa gaaggtcact taactaaaca
     2461 attacaatgg ttagaaggtt tctgtaatga tgaagattat gcaatggttg atatagctgc
     2521 tgcacttgta aaactatctc taggtgaaga aatgaaagaa gaaataatag aagaaaaacc
     2581 tagaagagag cgtggagata gaaaaggtgg tactggagct aaagatggta tgattagatt
     2641 atttatcaat attggtagaa atcaaagagt acaagcaaaa gatatagttg gtgctatagc
     2701 tggagaagtt ggaataccag gtaaagtagt aggtactata gatatatatg ataagtatac
     2761 ttttgttgaa atacctaaaa aagatgcaaa aacagtaatt gaaaagatga aagatattaa
     2821 gataaaaggt aataagatta atatagaaaa agcaaataag aagaaaaaat aacgtatagt
     2881 atacaaaact ataattagaa aatatataaa ataatatatt ggatatttaa aaagcctgat
     2941 gtgttttcaa aacagatagt agataatctg taagagttaa cagcataagg cttttcttgt
     3001 tttatatgat attatgattt tccagatcgt tgtgaaaaat gtatattagt tccttttttt
     3061 caaatctgat ttgaaaaaga gaatattaat tatctatata gtttagatta taataaaaaa
     3121 tgtaagataa caaaggggag aaaaagatgt caatgactta tatggaccct agggtttttt
     3181 tgattttaga aatggtgtta gaagaagaca caaatactct agaacaattt tcaaatgttt
     3241 tgggtgttag tactaggact atcagaaact atataaacca gattaatcga gaattaaaag
     3301 gtggtattgc taaaatagtt aaaaatgaaa aaggtacata tagtataaga attgaggatg
     3361 aaagtaaatt aattgagatt gtaaatttta atagaggaaa gctatcaaat tttataaatt
     3421 taaattcacc tgatgaaaga ataaattatg ttcttgatat tttaactatg accaacaatc
     3481 caattaccat agatgatttg gctgaagaga tatgtatagg aagaacaaca ttaataaagg
     3541 atttaaaaaa agcagacaga attttagctg aatatgattt agaattaaaa aggaagccaa
     3601 atataggtat gattttagtt ggtgaagaaa tggatataag attattaatc cttgatagat
     3661 tgtatgaaaa ttatattgat gtcttggaaa acgttaatca gataaattta gttaaagatt
     3721 atgatattga atgtcttaga gatgaattaa aaaagttgtt taaaaaagaa gaactctaca
     3781 taacagaaca ggtcttgaga gatgttgaaa gatatataat catgtctgtc ttaagaaatc
     3841 taaatgggta caaatttgaa aagatagata agagatttga agtgataaga tgttcagatg
     3901 aatatacttt aggattaaag ctcaaagctt tattggaaaa gatatttaat atagttttaa
     3961 atgaaaaaga aaccatattt ttgacaatgc cattaatagg tagaaaagct cctagtacaa
     4021 aattagcact tagcagtata aagataaatg atagcgtaaa ggaagttgta gatgaagtaa
     4081 ctagtttttt attagagacc tcagggattg attttaaaga agataaaaag cttatagaaa
     4141 atttggaata tcacttgtat tttgcactta atagaatgag attcaacata agggtaaaaa
     4201 atcctctttt acaagaaata aaggaaagat atacatttcc atatagtgtt gcaaagatag
     4261 ctgcaatgat aattgaggaa aagtttgact tgaaaatatg tgaccacgaa ataggatata
     4321 tagcattaca ttttggaagt tattttgaaa aaaattcaag aaaagttcta aatgtaaata
     4381 gggtagctgt tgtttgtgga acaggtcttg ggactgcaca acttattaga ataaagatag
     4441 gaaaaatact tggagaaagt gtagaagtaa atactttttc tgatatagaa gctaaagcta
     4501 atttagattt attaaagtat tatgatttag tatttacaac agtagatttc aaaacagact
     4561 tagatgttcc aatttttaga gtaaatgcgt tatttaatga agaaagtata aagaaagaga
     4621 tagaaaatgc aatacttttg aaaaatagta taaatcttaa gtctataaaa aagattgaaa
     4681 aaaatatccc ttttataggt aaattaattg aagaggataa gttctttata ttgaataaga
     4741 gtacgtttat ggaaaattta gaagatatgt tagaaaaatt agttcataaa ggaattattg
     4801 atatagagtt caaaaaaaag gtattaaaaa gagaaaagaa atcttcaaca gcattagata
     4861 attatatagc attgcctcac tcagttaata ctaatgggga aaatctttat ctgtcatttg
     4921 gaatattaga aaaaccagtg gtttgggata caaaggaaat tagaataata atattgatga
     4981 taattccaga taagaatgta gatagtacag acctgataat aaaaagttat gaagaagtac
     5041 ttgaagtagg gagagataaa aaaatgatag aagaacttgc taaagtaaaa tcatttgatg
     5101 aattttataa aataattacc aagaggaggt aataatatgg acccagtaat ttttattata
     5161 gcatcactta ttacagtgta tttattaaat atttttcttg gatacttaca gcttaaagat
     5221 tttaataaaa attatattga actaaaaaga aaaggaagag tggcaatagg cagaaaaaaa
     5281 ggaagaattt catcaggtac aattgtgttg atacttataa atgatgatgg tgtgattgtc
     5341 gaaactagaa aaatgcaagg tgttacagta ttggcaagag taaagtcatt tgaaggtttg
     5401 gtaggaaaga gcttagagta catagaaaaa tcagatttaa ctgtttacaa taaactactt
     5461 aaaaaagcaa tactagatgc agtcaaacag tacataacat ttgagaaaaa taaatgtgaa
     5521 gatacaaata atctgagtac tgatgaagag tgtaggatgg aagtagttta aaaaaatata
     5581 tttataagga ggagttaata tgacgcaatt ttttcaagta ttagcaagtg gtgcagatag
     5641 ttttatgaat ttatttaggg caggtgggca acaatttgta ggttttgtaa cagatatagt
     5701 accactttta gtagcattat tagttacaat gaatgcaatt atcaacttta ttggtacaga
     5761 tagagtcgag agacttgcaa aaaaatgttc tgcaaatata tttactagat atttgatatt
     5821 accagtcctt ggtacatttg tatttgcaaa cccaatgaca ctatcactag gaagattctt
     5881 gccagaaaag tttaaaccta gttactttgc agcagcaagc ttttcatgcc atacaatgaa
     5941 tggattattc ccacatatca atccaggtga actatttata tatctaggaa ttgcaaatgg
     6001 tataacaact ttgggattct caacagcaga tttagcagtt agatatttgt tagtgggtat
     6061 agttgcaaac tttattaaag gtgtagttac tgactataca acaaaatttg tagagaaaca
     6121 acaaggaata gcattaaatt cagatataaa gattggataa ttactttaag gtataaataa
     6181 atttttcaaa taaaaagata gtgtatagag gaggataaat tatatggcat ttaacaaagt
     6241 taaaatagta aaaggttctg gaggatgggg tggaccattg ataatagaac caacagaaaa
     6301 gaagaataag gtagtatatg tgactggggg agcaaagcca gaaactgcag taaaaatagc
     6361 tgaacttaca ggatgtgagt taatagatgg attttcacag ggtgttaaag atgatgaaat
     6421 cgcatgtgta ataataaatt gtggtggaac attgagatgt ggtatatatc ctcaaaagaa
     6481 aataccgact ataaatataa tgaaaacagg aaaaagtgga ccattagcaa tgtttataaa
     6541 agaagatata tatgtttcag gggtaaaacc aacaaatgta gagcagttat aattgaggta
     6601 gtaggaggga agaaatatgg cttatgatac taaaaagaag ataagtgagc agagtcaagg
     6661 aattgttgca aaaataggta tagcactagg aaatgtgaca agtactcttt ttcaagctgg
     6721 tagggatact atagatacat taatacatac tatactgcct tttatggctt tcgttgcagt
     6781 cctaataggt ataataaatc aatcaggtat aggaaattgg gtagccaatt tacttactcc
     6841 attaatcgga aatgttggag gtttagttgt cttatcaata atatgttcat ttccgttgtt
     6901 gtctccgttt cttggaccag gagctgttat agctcagata gtgggggtat tggttggtac
     6961 agaaatagga aaaggaaata ttccaccaag tttagcatta ccagcattgt ttgcaataaa
     7021 ctcacaagca gcttgtgact ttataccagt aggtcttggt cttgcagaag ctgaacctga
     7081 tacagttcaa gtaggagttc cgtcagtttt atattctaga tttttaacag gtgcaccaac
     7141 tgttctatta gcatggttag caagctttgg gttatataaa tagaagaata ctttataaat
     7201 aattagagag aggttcagtt gatatggaat taatatacag tactcaaata aataaaatag
     7261 gagataatgc aaatgaattt ttacaacata atatgtttgt aactttcaaa gataatgctc
     7321 ctcaagagtt agaaaattac tgttatatac attctgaaaa taatttagta aaagaaatac
     7381 ttacgaatga tgtgttatca ataaataatg ttgattacaa aattacttct gttggtgagt
     7441 tagtaaacca aaatctttca gaattaggtc atataacatt taagtttaca ggtgaaaaag
     7501 atggagcaat tggagggacc ttatatcttg aagaaaaaga aatagcacct attggcgtag
     7561 gtactatact aaaaataatt agaaaataaa atttaaaacg aatctaatca tattataagt
     7621 aaattataga taaaagtcaa tttaactaat caagttaact tttaatgtga taaaaaaggg
     7681 gaaatgaaaa tgagaaaagt ggctttagtt gttggaggag gaagaagttt aggtgaatat
     7741 ttatctaaac atttagctaa agtaggttat gatgttgcta ttgcagattt aaatgaagac
     7801 aacgcaaaaa aagtagctga agaaatagaa actgaatttg gttgtaaatc tgtaggatat
     7861 ggttgtaatg ccatagatga aagacaggtt ataagcactg ttaaagctat tgaaaatgat
     7921 tttgatagaa tagatttatt agtgtacaat gcaggagtgg ctgtaagtaa aaaaataact
     7981 gattttggat acaaagattt taaatttatt gtagacgtaa atttaaatgg gtactttcta
     8041 tttgcaagag aagtatctaa agtcatgata gcaaaaggta ttgagggtag aatcatacag
     8101 ataaattcaa aatcaggtag agtgggaagc aagcataatt caggatattc agcaggtaaa
     8161 tttgctggag taggacttac tcaaagttta gcgctagatt tggcagaaca taagataact
     8221 gtaaatgcac tgatgctcgg taatttactt gattctgata tgtttgaaag tttaattcct
     8281 caatatgcag taaaattggg aatacctgaa tcagaagtca agcaagttta tatagataaa
     8341 gtacctttaa aaagagggtg tagatttata gatgttgcaa atatgcttac attttatgct
     8401 tcaaaggaag ctgaatactg tactggtcaa tctataaata ttactggtgg acaagttatg
     8461 taaggttttt atggagtgtc tcaagatgaa ctctttagtt catgaggcac tttttttgta
     8521 taaaattaaa tatattttca tcatttaaac aatgaaaaag aggtttatct ttattttgag
     8581 atagcctcat ttttgtaatt aagaaacata gtgtttaagt aaacatataa gattgttagt
     8641 tttttaaaag agaatttttt aataatatta tatttatgaa gtatttttaa acttaatttg
     8701 attaaaaaat agctacgcac acttgtgact ttagtcatga gttagtagct aaattagtca
     8761 gcatataggg aaacttatat gtagagatag gatagaacct atccaatact acttgaattg
     8821 ctaaaaaccc ctaaagctaa ccaaactaaa acataagg
//
LOCUS       DAEPLF010000143         8841 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEPLF010000143 DAEPLF010000000
VERSION     DAEPLF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.143"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            527..2002
                     /locus_tag="KR314_002667"
     CDS             527..2002
                     /locus_tag="KR314_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBH0329038.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            2089..2502
                     /gene="speD"
                     /locus_tag="KR314_002668"
     CDS             2089..2502
                     /gene="speD"
                     /locus_tag="KR314_002668"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBH0329039.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2532..3383
                     /gene="speE"
                     /locus_tag="KR314_002669"
     CDS             2532..3383
                     /gene="speE"
                     /locus_tag="KR314_002669"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBH0329040.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3373..4251
                     /gene="speB"
                     /locus_tag="KR314_002670"
     CDS             3373..4251
                     /gene="speB"
                     /locus_tag="KR314_002670"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBH0329041.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4431..4631)
                     /locus_tag="KR314_002671"
     CDS             complement(4431..4631)
                     /locus_tag="KR314_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH0329042.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            5098..6588
                     /locus_tag="KR314_002672"
     CDS             5098..6588
                     /locus_tag="KR314_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0329043.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            6846..8342
                     /locus_tag="KR314_002673"
     CDS             6846..8342
                     /locus_tag="KR314_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0329044.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACISACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFEFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            8476..8826
                     /locus_tag="KR314_002674"
     CDS             8476..8826
                     /locus_tag="KR314_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0329045.1"
                     /translation="MKKIVLKDILFLVTTLSIIVILLFVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKNTAFKTTILSTGKSDCILIEIGDKFIMIDTGEDKNGEQIVDKL
                     KEKEINALDYLIKK"
BASE COUNT         3579 a          923 c         1563 g         2776 t
ORIGIN      
        1 gaataatata tttaaattaa catatataaa tcaatgaact tatagaattt taatatcata
       61 aatatagaat ttattttatc aattaaaaaa tttaataaat tttatattaa aattaaaata
      121 caaaaatagg aaaattattt agtccaacta aaccaagaat tttgatatat gaaataagaa
      181 taatataaaa gtttagtaat aaaatactta taaattaaaa atagtaaact caaaagctta
      241 ctaaaactca aaaaagtaaa aaaagcaaaa taataaaaaa taagaaagtt tataaatact
      301 aagttttaaa aaaattaata taaacaacaa aaaaataaaa agtttataaa tattgatata
      361 gctaatgtat atgtttttga gtaacttcaa aaaaacactt gtaaaaatgc tggaatgtta
      421 atataataaa tatcatgagt ttaataaaag taaaaataaa aaatagaaat aataaataat
      481 aaatattaaa aatatttgaa tatagagagg agatgaattt gaaatcatgg atagcgagtt
      541 tgcaaatgca gttgaattaa gctatgagtg tgctccatta ttagagtcgt taaaggaata
      601 ctcagagaaa gatatagctt gttttgatgt tcctggacat gtaaaaaata gaggtgtagc
      661 tatattaaat aaatatctag gtgaaagtct catgaaaatg gatataaatt catcaccaac
      721 tatggataat gtatctgcgc caaatgggat tataaaaaat gcacaagatt tgctagcaca
      781 agcatatatg gctgatgagg cattctttat aacgaatggc acaactcaag caatacatgc
      841 aatgattttg agtgttataa aaccaggtga aaaagtatta cttccaagaa atatacataa
      901 atcagtaata aatgcgttaa tactttgtgg aggaatacca atatttatac aaccagaatt
      961 tgatgaaaaa ctaggtataa gtttaaatat tactcttgaa aaagttagaa cagagataga
     1021 aaaagattgt aatataaaag cattattttt ccttaatcca acttactatg gagtttgtgc
     1081 agaccttgag agtattatag aactatgcca taaaaataat gttttagtgt tagttgatga
     1141 agctcatggt gcacattttc catttcattt agatttacct ccttctgcaa taagtttagg
     1201 agctgacatg gtagctgtaa gtatacataa aactggagga gcattgacac aatcttcagc
     1261 actcttattg aatagagata atgtaagttt tgaaaaggtt cttcaatcaa tcaatatgct
     1321 acaatcaaca tcagcatctt atttgcttat ggcaagtata gatggtgcta gagttaatct
     1381 agttgagaat ggtgaaaagc aactgtcaaa agcattaaat ctatctaggt atgcaaaatc
     1441 taaattgaac aaaattgatg gaataaaagt tttatctaca gaaatattga aacaaaaagg
     1501 tgttaaattt atagatgaaa caaaattgtg tataaatgta aaagaattaa atttgactgg
     1561 atttgaagta tacgatttat tatataaaaa tttttcaatt caagtagaat tgggagattc
     1621 atataatata ctggcacttg tgtccattgg aacaaataaa tcagatatag atagattagt
     1681 aaaagcactt tctataattg caaagattta tagaaaagaa tcaacattaa atgaatttaa
     1741 tatggttcaa ataaatccaa ttattaaact taatccaaga gaagcttttt atgcaccaaa
     1801 ggaaagtgta gaaatcaatt catgtataga tagaatttgt ggagaatcaa taatggcata
     1861 tccaccaggt ataccgatta ttgctccagg agaattgata acagaagaaa ttatggagta
     1921 tattattttc ttaaaaaata gcaatgcgta tcttacagat gtacaagata aaaacttaga
     1981 tagaatacta gtaataaaat aaattacttt attttgcaag ggcaagtgtt tgttaagtag
     2041 agtttatcaa atagaactta ttaaaagtat aaaggagttg gggttgaaat gaaatttgaa
     2101 acactaggaa gacatatact agtagaatac tataattgtg atgaaaacgt acttaaagac
     2161 ccacgtctta tagaaaattt tatgaatgat tcagcattaa atgcaaaagc gacaattgtg
     2221 gattctgtat tccaccattt taatccttgg ggggtttcag gagctgttat aattgctgaa
     2281 tctcatttga caatacatac atggcctgaa tatggttatg cagcagcaga tttttttact
     2341 tgtggtgata tagacccatg gaaaagtttt gaattattag agcaactttt aaagtctgaa
     2401 agaagcgagt ctacagaaat tccaagaggc ttaactacaa aaattaaaaa atattcaaaa
     2461 aaagatttag gtaagattac tcataaacca gaagcagtat aaaacataca agagaaagtg
     2521 aggattatta tatggaactt tggtatacag aagaatggac agaaaatgta agattttcta
     2581 taaaagtaaa taaacatcta tttgaaggaa agtctcaatt tcaaaggata gatgtatttg
     2641 atagtgatga atttggaaaa tttctaacaa tagatggatt aatgatggta acatataaag
     2701 atgaatttat ataccatgag atgataactc atgttcctat ggctacaaat ttaaacataa
     2761 agaaagtatt agtcataggt ggaggtgatg gaggtacagt aagagaatta agccgttatc
     2821 ctcaaataga aaagattgac atggttgaaa tagataaaat ggttgtagat gtatctaagg
     2881 agtatatgga tatatgctca tgtaaattag atgataaaag agtgagttta tattttgaag
     2941 atggtgttaa ttttgttaag tgtgcacatg ataaatcata tgacctgata atagtagatt
     3001 caactgaccc aataggacca ggcgaagggc ttttctcaac tgatttttat aaggattgtt
     3061 atagaatact gactgatgat ggaattttag taaatcaaag tgaaagtccg tattttgact
     3121 ttaatgctaa agagatgaaa agagctaata aaaaattaaa acagatattt ccaatatcag
     3181 aagtttatca agcacatata ccaacatatc catctggtca ctggttgttt ggatttgctt
     3241 caaaaaaact taatccagta aaaaatcaag atagaaatgg atgggagaaa ttaagtttaa
     3301 aaactaaata ttataatagt gatatacatt taggttcgtt tatgttacct caatacgtga
     3361 aggagatgct agatgaagaa taatttctat catatgaaca cttttatgag tatggacaaa
     3421 aattatgaag aatctaatct tatagtattt ggtgttggat ttgatggaac tacttctaac
     3481 agacctgggg ctagatttgc aagtagctct atgagaaaag aattttacgg tcttgagaca
     3541 tatagtcctt ttttagattt ggacttagag gattataata tatgtgatta tggagattta
     3601 gaaattagtg ttggaagtac agaacaagtc ttaaaagaaa tctatcaaga gacatataag
     3661 attgttagag attcaaaggt accctttatg attggaggag agcatttagt tacattacca
     3721 gcctttaaag cagtacatga aaagtacaat gatatatatg taattcattt tgatgcccat
     3781 actgatttga gggaagaata taataatagt aaaaattctc atgcaacagt aattaaaaga
     3841 atatgggata ttgtaggtga taataaaata tttcaatttg gtataaggtc tgggacaaaa
     3901 gaagaattta aatttgctac agaagaaaaa cacacataca tggaaatagg aggaatagat
     3961 acgtttgaaa atatagttaa tatgctaaat ggaaagaata tttatctaac tatagattta
     4021 gatgtattgg atgcatctgt gtttccggga acaggtacac cagaacctgg tggtgtaaat
     4081 tatagagagt ttcaagagat ttttaagatt ataaaaaact ctaatataaa tatagttggt
     4141 tgtgatattg tagagttaag ccctgattac gatacaacag gtgtatcgac agttatagct
     4201 tgtaaaatct taagagagtt atgcttaata atatctgata aaattaaata gagtatttta
     4261 tataaatagt atatattata atataagaag ttattttaaa aaagttagtt taagtttgac
     4321 gcttttctaa agtaacttct tatttttgta attaaaaata aatataataa acaacaaaaa
     4381 agagccctcg atagctcctt ttctaaaata tatatattta aattaaataa ttatattcta
     4441 tttacgtttt cagcttgagg tcctctatta ccttgagtta tatcaaagct aaccttttcg
     4501 ccttcttcta aagttttgta tccatcattt tgtatagctg agaaatgtac gaaaacatca
     4561 tcttctccct ctacagatat gaacccaaat cctttttcat tgttaaacca ttttactatt
     4621 ccgtttttca ttataagaaa acctccgcat tattaaaaaa tacaaactaa aaaaataagt
     4681 ctgttctatt aatataacat aaattttaaa taaaagcaaa taaaattttt aaaaaaatta
     4741 attactttta gaatttaata aaaattgtta aattatacag tgagaagtat tgtaaaattt
     4801 gacgaaaatg taaatgtaga taataaattt aatgtcaaaa aatgcaacaa aaaggtatgt
     4861 aagtaaaaag taaaattaaa atatataata aaaaatatta tatattagat taaaaagtaa
     4921 aaataaaaat agtgaaattt cagaaatatc aataatatta tataataaaa taaaaaaaca
     4981 ctatatatat tcgataaaat aaaatttaaa catataatat tttatgtata tattaataaa
     5041 aggacatatt gtgttataat ggtatcgtat aaaatcattt acgggggtgg tattaaaatg
     5101 tttcaatttg aaacacaaat acacaaatta agacatgatg ttttaactgc aatagctaga
     5161 cttggaaaag aagataacct taccagagaa gcaatggata atattcagta tgaaataata
     5221 aaaggtgaga aacctacata cagatgttgt gtttataaag aaagagctat aatatctgag
     5281 agagtaaatg taagtatggg gttacaacca ggtcaagata agatagattt agatacaatg
     5341 gatatagaag aagatgaaca aattatatat attttggaag ctgcatgtga cagttgtcca
     5401 ataaatgaat ttacagtaac agatatttgt agaggatgtc tagcacatag atgtaaagaa
     5461 gcttgtaaat ttggagctat aacacatgtc ggaggaatgg cctatataaa tcatgaatta
     5521 tgtaaggctt gtggaatgtg taaaaaagct tgtcaatatg atgccatctc agaggttgtt
     5581 agaccatgta agagtgtttg tccaactaat gccttaggat ttgatagaga aaatatgaaa
     5641 gctatgatac atgaggataa atgtttaaac tgtggagcat gtatgtcagc atgtccattt
     5701 ggagcaatat ctgataagag tttgatagct cctgtagcta gaaagttggt tcaaaaagag
     5761 aaaatgtatg cggtagtagc tccagctata acaggtcaag ttgaagcaaa tgttacatat
     5821 ggtcaaataa aaaatgctat aaaatcacta ggatttgttg atgtattaga agcagcttgt
     5881 ggagcagatg cagttacagt tcatgaaagc ttagaatttg ctgaaagaat ggaaaaggga
     5941 gaggctttta tgacaaattc ttgttgtcca ggatttgttg gttatataga aaaaacatac
     6001 ccaaatcatg taggaaaaat atcttctaca gtgtctccta tgatagctag tggtagattt
     6061 ataaaaagtc atgaaccaga tgctaaagtt gtattcatag gaccatgtac agccaaaaag
     6121 agtgaggcag ctaagaaaga attaaaagga gctgttgatt atgtacttac ttttgaagaa
     6181 ataatggcat tatttgaggc atttgatata gacttggcta agtgttcaaa tgaagatata
     6241 gatgatgcat caatttttgg aagaggtttt ggagctgctg gtggtcttac aaaagctata
     6301 gaaaattatc ttgctgaaaa aggtattggt gttaattttg aacctgtaaa gatttctggt
     6361 tcaagagaaa tcaagaaaac tatgactatg gctaatttag gaaagctaga aggtaatttt
     6421 atagaaggaa tgatgtgtga aggtggttgt ataaatggtt ctggtaaatt cttatcaggt
     6481 gcaaaggtta aagccacttt tgatagaaaa aattctcaat caactaagaa aagtgtctta
     6541 gcaaatacaa agataaaaga ttttagtaat ataaatttag aaaaataaag ataatacaaa
     6601 gcaacaaaaa tacatattgt taatagagtt gttctaagtt gaggtttaac ttttggacaa
     6661 ctctattttt atttatatat ttaattaatg aacaactaaa ataaaaatta catatataat
     6721 attttgtata taacttaaca aagtatatgc ctatgttata ataatttata gaaataaata
     6781 aaaaatacaa tatacataat agtagaaata tagtaaaaag gtgtgattta tagggaggat
     6841 tacttatgtt tgaatttgaa acgcaactaa aaaggttgaa acatgaagtt ttaacagcag
     6901 tagcaagact atctattgaa gaaaatttaa caaaagaaaa tatagaaaaa attccatatg
     6961 aaataattaa aggagaaaag gcgagatata gatgttgtgt ttataaagag agagaagtag
     7021 ttcttgaaag ggcgcaactt gcattaagtt taactccaaa tagtaaatat ggagatataa
     7081 atccagattc agttagtttg gacacagatg agcaaattat atatatagtt gaagcagcat
     7141 gtgatagatg tccaataaat gaatttacag ttacagagct ttgtagaggg tgtttggctc
     7201 atagatgtaa ggaagcatgt aaatttgggg caatttctta tataaatggg agagcatata
     7261 taaatcatga tttatgtaag gcttgtggaa tgtgcaaaag ttcatgtcaa tatgatgcta
     7321 tatcagaagt tgtaagacct tgtaaaagcg tttgtccaac aggagctttg gattttaata
     7381 gaaacactat gaaagcaatg atacatgaag ataattgtat aaattgtgga gcatgtatat
     7441 cagcatgtcc ttttggagca atatctgata agagtttgat agcacctgtt gcaaaaaaac
     7501 ttgcaaagaa agaaaatatg tatgcaatag tagctccagc tataacaggt caaatagata
     7561 gtaatattac atatggtcag ttaaaaaatg ctataaaatc acttggattt gtagatatga
     7621 tagaagcttc ttgtggagca gatgcagtta cagttcatga aagcaatgag cttattgaaa
     7681 gaataaaatc aggagataat tacatgacaa attcatgttg tccgggattt tttaagtata
     7741 tagagaagat gttcccagat gaaaaagata gaatatcatc tacagtgtct cctatggtag
     7801 caacaggaag attagtaaaa aaaatagatt ctgatgctaa agttgtattt atagggccat
     7861 gtacagctaa aaagagtgag gcaacagaga aaagtttaaa agattcagta gattatgtac
     7921 ttacttttga agaactaatg gcattatttg aagcatttaa tatagattta tcaaaatgtt
     7981 caaatgaaga tataaatgat ggttcgattt ttggaagggg atttggagcg tctggtggac
     8041 ttgccaaatc agtacagaac tatattgctg aaaagggact tagttttgag tttgaaccta
     8101 taaaagtatc tggtccaaga caaattaaaa agacaatgac aatggctaaa ttgggaagat
     8161 tagaaggtaa ttttatagaa ggaatgatgt gtgaagaagg ttgtattaat ggagcaggta
     8221 agtttttatc aggtgtaaaa gctaaaaata cttttgatag aaaaaatgga caatctacta
     8281 aaaagagtgt tttatctaat gataagatta aagaatttga agatgtaaat ttagaaagat
     8341 aattgttttg gtacagtcat ttttttatca tgccacaaat atgcctaaaa ttttcccttt
     8401 ttatatgtgt attttacaga gttaaggttg ctatactata aatatgtaat aaataaaatt
     8461 tataggagct tttggatgaa gaaaatagta ttaaaagata tcctgttttt agttactaca
     8521 ttatctataa tagtaatatt attgtttgta acaaatataa ctactaatag taagataaaa
     8581 tcaacaagtg aatcttattt aaaaagtgac aaaaatagtg tgaggcaaaa tggtatccag
     8641 aggagcacaa aaaatactgc atttaagact actatattaa gtactggaaa gtctgactgt
     8701 atattaatcg agataggtga taaattcata atgattgata caggagaaga taaaaatgga
     8761 gaacaaatag ttgataaatt aaaagaaaaa gaaataaatg cacttgacta tttgattaaa
     8821 aaatagctac gcacactcgt g
//
LOCUS       DAEPLF010000144         8773 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEPLF010000144 DAEPLF010000000
VERSION     DAEPLF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.144"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            45..110
                     /locus_tag="KR314_002675"
                     /pseudo
     CDS             45..110
                     /locus_tag="KR314_002675"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(626..1294)
                     /locus_tag="KR314_002676"
     CDS             complement(626..1294)
                     /locus_tag="KR314_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBH0329046.1"
                     /translation="MPVLMAIGVPVHLAIGTNKFAASAGCISSAYRYAKSGKINNDLL
                     KKLVPFTIIGSVLGVRCVLSISEEILNVLVVVMILIVAIYTFISKNLGQEDNFEAVNK
                     KNLRLGMLMALIMGFYDGFFGPGTGTFLTFGFIKIYGYDFLHASANTKILNLTSNITS
                     LLLFMINGQVDYKIAIVFALVMIMGAYVGAKVAIKKGSKMIKPIFLVMALFMVVKLVY
                     QTLV"
     gene            complement(1239..1379)
                     /locus_tag="KR314_002677"
     CDS             complement(1239..1379)
                     /locus_tag="KR314_002677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329047.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGTYKHACSYGNWSASTSCHW
                     YK"
     gene            complement(1438..3093)
                     /locus_tag="KR314_002678"
     CDS             complement(1438..3093)
                     /locus_tag="KR314_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0329048.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQMIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKGI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVDSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(3322..3690)
                     /locus_tag="KR314_002679"
     CDS             complement(3322..3690)
                     /locus_tag="KR314_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0329049.1"
                     /translation="MKYSITDVSKITKISSSAIRFYEEKGLLLPINRKPSGVRYFSEE
                     DVERLIFIKELKNTGLSIKDIHECFHFCDMGDGTIDKRIQLFQQHKQNIKNKIHELES
                     CMDKIDEKIYYLKEKKMCKK"
     gene            3839..4633
                     /locus_tag="KR314_002680"
     CDS             3839..4633
                     /locus_tag="KR314_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase"
                     /protein_id="HBH0329050.1"
                     /translation="MEKYATMIKEKLNEGKVLCGVAVSLSDSVVSELIGISGYDFIWI
                     EGEHSALDRKDIQQHMIAAHAGGAASLVRLVNSDPDLVKPIMDMGTDIICFPFINTVE
                     DAQKAVAACTYPPNGIRGYNPQRASYYGNMNYFDYVKNANNETLKMMLIEQKAGYDNL
                     EEIVKVEGIDIIALGPGDLSLDMGFSGYMNRIEIKDMISHAAEICKKYKMPFIIFPTI
                     EKKSIEEWVNIGANMLCFAQDTTFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(4786..5214)
                     /locus_tag="KR314_002681"
     CDS             complement(4786..5214)
                     /locus_tag="KR314_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH0329051.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(5690..6628)
                     /locus_tag="KR314_002682"
     CDS             complement(5690..6628)
                     /locus_tag="KR314_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBH0329052.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
     gene            complement(6972..8627)
                     /gene="ilvD"
                     /locus_tag="KR314_002683"
     CDS             complement(6972..8627)
                     /gene="ilvD"
                     /locus_tag="KR314_002683"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBH0329053.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIEFNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
BASE COUNT         2895 a         1528 c          888 g         3462 t
ORIGIN      
        1 aaatatactg tataaaaatg cataaaaacc tctactaatt agatacaact ataatagaat
       61 atatttatta ttataataac aaaagaatta aaaaaattaa tttagcataa ccttgttaaa
      121 tatagatttg aatatgaatt taaagttatg taaaattaaa aatacaggaa tgaaaattcc
      181 attcctgtat aaaatctaac ttttgagtct caatacaatc ttattttgag acaatctttt
      241 ttgttttata ttttcttata tttaaaatat aacgcatatc ttatgcaaca tattcatatt
      301 tttctttata ttctttcatc gcatcattcc aagcgtcttg tactgttaca cctcttttat
      361 tagcaatttt ttgtgccagt tcaattatct tcatagctct tcttgatatg ttcataaaca
      421 ttcctcctac aaattaccat atatttcata tatataaatc aaggttatat ttttatgata
      481 tataatcctt ctattgtaat attacaattt atatgtgtat tatatatgaa ctatattaga
      541 agataattag aatttttata aagctatatg attagattaa atataaaagc tttacaatta
      601 cttataaatt taaaaagtct ataaactaaa ctaatgtttg atataccaat tttacgacca
      661 taaataatgc cattactaaa aatattggtt ttatcatctt tgaacctttc tttatagcaa
      721 ctttagcacc tacataagct cccattatca tcactaatgc aaacactata gcaattttat
      781 aatctacttg accatttatc atgaataata acaatgaagt tatattactt gttaagttta
      841 atatttttgt atttgcagaa gcatgtaaaa aatcatatcc atatattttt ataaatccaa
      901 aagtcaaaaa agttcctgta cctggaccaa aaaaaccatc ataaaaaccc attataagag
      961 ccataagcat ccctagtctt aaatttttct tattaacagc ttcaaaatta tcttcttgac
     1021 ctaaattttt agaaataaag gtgtaaatag ccactattaa aatcataacc acaaccaaaa
     1081 catttaatat ctcttcactt attgataata cacatctcac acctagaaca ctaccaataa
     1141 tagtaaatgg tactaatttt tttaaaagat cgttattaat ttttccagac tttgcatatc
     1201 tataggcact acttatacat cctgctgatg ctgcaaattt atttgtacca atggcaagat
     1261 gtactggcac tccaattgcc ataagaacag gcatgcttat aagtccctcc tcctcctgct
     1321 atagaatcta caaaagcagc taaaaacccc cctatacata aaaaaataac tgaaaacata
     1381 aaattcctcc atcattctct ttcaattttt cttacaatat atattatata atcttattta
     1441 atacttatta tccagcatat tctttctata ttcatttgga gttatactat taaacttctt
     1501 aaatatttgt gaaaaataac tactgttatt aaaacctact ttcttacaaa tatcactaat
     1561 actcatgctt ctatctaaca gatatttttt agattcttct atcctaagaa gatttatata
     1621 ctcagttatt gaaactttat ttctttcatt aaaaatagaa ctcaaataat ttggacttat
     1681 aaaaaaaaca tctgatatac tattcaaatt tatattttct ttataatatt tttttatata
     1741 ttcgtttata ttttcaatta gatttccttt atcttttctc caaaatgctc tattcttatc
     1801 aatacactct ttggaaaatt caaataatac attccataaa tcttcatcac tgtctacaag
     1861 tgatattttt ttattatatt tatttcttaa ataagatata agttttaaat cctctaaatc
     1921 tctaagcact gttctagaca atacaaatat catttcctga atagtatctc taacatcatt
     1981 atatcttaat ttgtttgtat ctattagatt ataaacacct tctaaattca tatttttaat
     2041 ttctttttca atataaagtt ctaattcaaa gggatattct tttgaagaat tattgaacat
     2101 atctatcgga ttaataatat actcttcttc taataatcta ctatttgctt ttttaagtgc
     2161 ctcttcaagt cttatatttt cttttctact ctttaatatc tcctcatctt ttatactaag
     2221 ttgattttca aaaaaattcc tcttagctat ttcctgaata aattcagaca tcattaataa
     2281 attttctttt aatacatata atctactttt aggatttaat ggaaattcat tgtaaatttt
     2341 gtttatgtat tttatatcaa taccagaact acttgataat tcagataaat ttttatctat
     2401 atcaatagac ttatatacta taaaattacc acattctata tatccaacta tattttcttc
     2461 taatactata ggtatctcta aactggaaac accatactca caatggtaca ctttttcaaa
     2521 gttatcatta tttatgcttt taacaagttt tttagtacac ggttttatat ttttacaaat
     2581 atcgcaaaat ttattttctt tcttagtctc aaatattcct ttatacttta agtcatatac
     2641 agatattcta agcttcaata tattagatat ttgatttgca agattttcaa ttatgtttac
     2701 attaactaac tcttgtatat tgttttcaga aaatactata ggtatttgtc tcaaactcga
     2761 aaatttatga ggaacataag taattaatat tactaaatca attttaccag tgtttataat
     2821 ttcatgttct gaatatggtg ggcaataaac tatagatgtt tcctttatat tgaccttttt
     2881 accatcaatc atttgtattc cttctccctg aatcacatat aaaagctgtt cttcacttaa
     2941 atgaaaatgc ttttcttggc agtttcccgg aaaaaatctt acaattccag cactaagtct
     3001 attgaattgt gtttttgatg gttcgtgtat ccacataacc tctccccaat caaatttttg
     3061 tacttcatta tttgactcaa taaaatccac cataaactca cctcgtagtt tttatctaaa
     3121 tattttttta ttcctttaaa tatctcctta taatatacat tatcataatt ttctttttta
     3181 ttgtatagat aaatagttat atagaacatt gtccaattct tcctaaaaat atagtgtaaa
     3241 aaatctttcc tcaaatgttt ccaattgaat ttactcttta tattagaaaa atcaaaaaat
     3301 tatttcttta ttctataagg attatttttt acacatcttt ttttctttta agtaatatat
     3361 tttctcatct attttatcca tacaactttc tagttcatgt attttatttt taatgttttg
     3421 cttatgttgt tgaaataatt ggattctttt atctattgta ccatctccca tatcacaaaa
     3481 atgaaagcat tcatggatat ctttaataga taaaccagta ttttttaatt ctttgataaa
     3541 tattagacgc tctacatctt cttcagagaa atagcgaaca ccacttggtt ttctattgat
     3601 aggaagtaat aaaccttttt cttcataaaa tcgaatagca gatgaagaaa tttttgttat
     3661 ttttgataca tcagttatag aatatttcat aatctctacc tccctaaatt ttatatgaat
     3721 aagaatatta taaaaatacc ttatacagta gtcaacaaaa aagtattgac ttcaagttag
     3781 gttgaagttg tactctttga agtgtaaaag aaaacttaca aaagaaagga tgatataaat
     3841 ggaaaaatat gcaactatga ttaaagaaaa gctaaatgaa ggaaaggtct tatgtggagt
     3901 agctgtttca ctgtctgatt cagttgtaag tgagttaatc ggtatatctg gatatgattt
     3961 tatttggatt gaaggagaac atagtgcttt agaccgtaaa gacattcagc aacatatgat
     4021 agctgcccat gctggtggtg ctgcttcttt ggtacgatta gtaaacagtg accctgacct
     4081 tgttaagccc ataatggata tgggaacaga tattatttgt ttcccattta ttaatacagt
     4141 agaagatgca caaaaagccg tagcagcttg tacttatcct ccaaacggta taagaggata
     4201 taatcctcaa agagcctctt attatgggaa tatgaattat tttgactatg tcaaaaacgc
     4261 aaacaatgaa acattaaaaa tgatgttaat tgaacagaaa gcaggttatg ataatcttga
     4321 agaaatagta aaagtcgagg gtattgatat tatagcatta ggccctggtg acttatcttt
     4381 agacatgggt ttttccggat atatgaatag aattgaaata aaagatatga tttctcatgc
     4441 tgctgaaata tgcaaaaaat ataagatgcc ctttattatt tttcctacaa tagaaaagaa
     4501 aagtattgaa gaatgggtta atattggagc aaatatgctt tgttttgcac aagatactac
     4561 ctttatcact tatgttatca atcaattatt atctttttat aatgagattg taccaattca
     4621 aaaacaacat taaattataa atctaatttt atattaaaag agactaatat tttatctatc
     4681 tctattaagg atattttctt atgtatcata atattagtct ctcttattat tatatactgc
     4741 tatcatcatt agttgctatt tatactattt ttataatata caaatttatt cagctaacgt
     4801 tttgcctaat tctatacact catcttttgc ttcttcattt gggtcttcca ttgctgtaat
     4861 tccttctcca ccaataattg tagcaccagc attttgcata cgttcttccc agtctctcat
     4921 ccattcacaa tttccccatc catatgaacc aaataatcca atctgtttac ctgataccat
     4981 agattctaat tctgaaacaa atggctccat atacccctct tctagttgct ctgctcccat
     5041 agatgggcaa cctaatgcaa atttatcttc ttcttttaaa tcacttggtt tcagtaagct
     5101 cacatctaaa acttctggtg tcttaccttt taacttcaca ccttcagcca caaaatttgc
     5161 cattttttca gtatttcctg ttccactcca ataaacaata tatattttac tcatttttac
     5221 ctcctgtagt attttttaat taatcaatag ttagttatag ctaactaaca tctaaaaaac
     5281 aaaagatttc tttatctgaa atctctttat ttttctaaat atagaatatc acaattgatt
     5341 ttgaaattca atatttattg ataatattta tcatttatat tttatattca ttttataata
     5401 tcaaaacact gtatcgtatt tctaatcttt catagaaaaa tttctaactt atagtacttt
     5461 tgcatctctt ataaatcttt tatatgtata agtacataga aaaacacacc ttttccagat
     5521 gtgctatcta taatatcgta tatttatttt tcaattctaa ctagctaata tgtttataaa
     5581 ttttatcact taataccttt atacattgat gtaaaatctt acacctaagt aatttttatt
     5641 atttgtctcg actctatata atatagctat tttatcatca aatcaaaaat tatcttttgc
     5701 tattattctt gtatttataa agatttaaat ataagtatcc atttccttgt tccgttctag
     5761 gtattccaag cgctctcgta cattttatta attgtgcata cagttcagct tcatctaaat
     5821 ctctttcaaa ctcctctctt gcccattctt taattagtgc taatgagcct gatacgtatg
     5881 gtgcactcat actagtacca ctcataatag caagtttatt atccatataa gtcgatataa
     5941 tatttcttcc tggagccacc aaatctattg tagtatttga attactaaac ttttcaacca
     6001 aatagttttc atttattgca cctacttcta ttacctcagc ataactggct ggataactat
     6061 actcacttgt actagaatca ccatctccat tattacctgc tgcacacact acagaaatat
     6121 tattttttac tgcttgcatg acagcatttt ttaaattctt atcatctttg tttcccccaa
     6181 gagacataga tataatatca accttgttat ttacagcaaa gttaatagca ttaattatac
     6241 tttgatatgt tccggtacca tctttattta atgcttttgc tattaataat ttacaatctg
     6301 gagctactcc cataacttca ttattatagt tagatgcagc tataataccc gccacatgag
     6361 ttccatggcc attaaaatcc tcatatatat ttttatttcc attactgtca tcactaaaat
     6421 ttgcaccacc aattattttt ccttttaaaa gaggatgaga tatatcacaa cctgtatcta
     6481 ttataccaac tacaatattt ttaccagtat aaccttcatc ccacattccc ctagcattca
     6541 tttgttttat tccatatggg aatttatttt ttgcacctct tagattttca tttatttcat
     6601 acggaattag tttcattctt ttattcatca tatatcctcc taaaaatttt attgcttata
     6661 aaaatcatga attttatttt ataataggtt tataaatcaa attaaaacct atatctactt
     6721 atttatattt atgatatatt ttttattttt aggagtcata tttttttgta tataaaataa
     6781 tatttagata actatattaa aaaatacatg tttgcttata tacaaaactt actaaaatat
     6841 ctttaatatt tttgtataca aacagtatat ttttcataca aaataaaaag gatacctcat
     6901 tagtgctata acacctaaga tatccaatta atattttctt aattttttct aattttcgta
     6961 aatatagttc actattttaa aactgctcct gtatttgctg atgttaccaa tttagcatat
     7021 ctacttaagt atccatgctt tattttcggt tctaaaggtt taaattttaa ttttctattt
     7081 tctatttcaa cttcatctac ttttaactct aattttttgt ctggtatgtt tatagaaatt
     7141 atatctcctt cttcaactag tccgattaaa ccaccttcca tagcttctgg agaaatatgg
     7201 cctatagatg ctcctctagt tgctcctgaa aaacgaccat cagtaagaag tgctacatgc
     7261 ttatcaagtc ccattcctgc aactgctgat gtaggagata gcatttcttt cattcctgga
     7321 ccaccctttg gaccttcata tcttataact ataacatcac ctttgtttat ttttttacca
     7381 aaaatagcat ttacagcttc ttcttctgaa ttaaaaactc ttgctggtcc ttcatgaact
     7441 aacatttctt ctgcaactgc tgactttttt acaacagctc catttagagc aagatttcct
     7501 ttcaatattg caagcccacc ttggttacta tatggattct ttaatgtatg tattacttct
     7561 tcattttcta tttcacaatt tcttatattt tcccctatag tcttgcctgt tacggttttg
     7621 caatctaaat ttattccatt tatctttgaa agttcgttca ttatagctgg tattcctcct
     7681 gccatatgta aatcttcaat atgatgtttt ccacttggac ttaattttgt taaacaagga
     7741 gttttttcac ttatttcatc aaaaaaatct aagttaaact ctattcctga ttcataagct
     7801 attgcaggta agtgaagtac tgtatttgta gaaccagcca ttgccatatc aacagctata
     7861 gcatttttaa aagcatctat tgttaaaata tctctaggtt ttatgtcgtt cttaagtaac
     7921 tccataacat acatacctgc atattttgct attctttttc tttctccaga atgtgacgca
     7981 gcagtaccat tatatggtat acccatgcct aaagcttctg ttaaacaatt catgctattt
     8041 gctgtgaaaa gtccagagca tgaaccacat ccaggacaag agttcatggc taactcttct
     8101 aattcttctt cacttactgt tccatcttta catgctccaa caccctccat tgcagagtta
     8161 aaatcatata cttttccatt ttttttacct ggaagcatag gccctccact cacaacaata
     8221 cttggtatat taagtcttgc agcagccatt aacatacctg gcactatctt gtcacaatta
     8281 ggtataagta ccaaagcatc aaaaccatgt gccatagcca tagcttctat tgaatctgct
     8341 attaattctc ttgaagccag ggaataattc attccgacat gccccatagc tataccatca
     8401 cataccccta tagcaggaaa ttctaatggc aaaccaccac tcattcttac tccatttttt
     8461 gcagcttctg ctatttcatc aagatgcaga tgaccaggaa tcacctcatt ttgtgcattt
     8521 actataccta ttaatggtct ttcaatttct tctttagtaa gacccattcc atacattaac
     8581 gctctctttg gagctccttc tattcctttt tttatatcac ttctcataat attacctctt
     8641 tcttactttt ttaaattttc ataatattat tataatatat ttttctatta attaattacg
     8701 aaaattgtaa aaaaatatta gcatatacta atgaaaattt attacgattt tttatcaata
     8761 tcttacacaa tcg
//
LOCUS       DAEPLF010000145         8766 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEPLF010000145 DAEPLF010000000
VERSION     DAEPLF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.145"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            354..1757
                     /locus_tag="KR314_002684"
     CDS             354..1757
                     /locus_tag="KR314_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0329054.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFMLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     TSLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1848..2088
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2131..2829
                     /locus_tag="KR314_002685"
     CDS             2131..2829
                     /locus_tag="KR314_002685"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBH0329055.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILKGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2852..3853
                     /gene="trpS"
                     /locus_tag="KR314_002686"
     CDS             2852..3853
                     /gene="trpS"
                     /locus_tag="KR314_002686"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBH0329056.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANAIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(4505..7135)
                     /gene="cdtB"
                     /locus_tag="KR314_002687"
     CDS             complement(4505..7135)
                     /gene="cdtB"
                     /locus_tag="KR314_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094607992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin binding subunit
                     CdtB"
                     /protein_id="HBH0329057.1"
                     /translation="MKIQMRNKKVLSFLTLTAIVSQALVYPVYAQTSTSNHSNKKKEI
                     VNEDILPNNGLMGYYFTDEHFKDLKLMAPIKDGNLKFEEKKVDKLLDKDKSDVKSIRW
                     TGRIIPSKDGEYTLSTDRDDVLMQVNTESTISNTLKVNMKKGKEYKVRIELQDKNLGS
                     IDNLSSPNLYWELDGMKKIIPEENLFLRDYSNIEKDDPFIPNNNFFDPKLMSDWEDED
                     LDTDNDNIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNTAGDPYTDYEK
                     ASGSFDKAIKTEARDPLVAAYPIVGVGMEKLIISTNEHASTDQGKTVSRATTNSKTES
                     NTAGVSVNVGYQNGFTANVTTNYSHTTDNSTAVQDSNGESWNTGLSINKGESAYINAN
                     VRYYNTGTAPMYKVTPTTNLVLDGDTLSTIKAQENQIGNNLSPGDTYPKKGLSPLALN
                     TMDQFSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGTKNSSGQIVTEGNSWSDYIS
                     QIDSISASIILDTENESYERRVTAKNLQDPEDKTPELTIGEAIEKAFGATKKDGLLYF
                     NDIPIDESCVELIFDDNTANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNFDD
                     YNNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIPMSELKPYKRYVFSGYSKDPLTSN
                     SIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTTYLDNLSITE
                     LNSTPEILDEPEVKIPTDQEIMDAHKIYFADLNFNPSTGNTYINGMYFAPTQTNKEAL
                     DYIQKYRVEATLQYSGFKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIMTYK
                     KLRIYAITPDDRELLVLSVD"
     gene            complement(7188..8579)
                     /gene="cdtA"
                     /locus_tag="KR314_002688"
     CDS             complement(7188..8579)
                     /gene="cdtA"
                     /locus_tag="KR314_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin enzymatic subunit
                     CdtA"
                     /protein_id="HBH0329058.1"
                     /translation="MKKFRKHKRISNCISILLILYLTLGGLLPNNIYAQDLQSYSEKV
                     CNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKY
                     SQTRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTEN
                     QNEISLEKFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLP
                     YTNTNNVSTLIEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSWGKA
                     NYNDWSNKLTPNELADVNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKR
                     EPIPTNLTVYRRSGPQEFGLTLTSPEYDFNKLENIDAFKSKWEGQALSYPNFISTSIG
                     SVNMSAFAKRKIVLRITIPKGSPGAYLSAIPGYAGEYEVLLNHGSKFKINKIDSYKDG
                     TITKLIVDATLIP"
BASE COUNT         3010 a         1148 c         1300 g         3308 t
ORIGIN      
        1 acggaagtaa aatgactttt tattgtgaaa attctataaa agtaataaat taagtctata
       61 aagttatgta ttttatcttt ttatatacta atgaaaaata ataaataaaa ttttataata
      121 aaataatatt gaaacaattt taaaaattta gtatcattag aattaaagtg gtaaaacttt
      181 taaatttatc atttttaata agatttgatt tcaaagtatt taaggtttat tattaaaact
      241 gtttttgaat atgcaaatat tttgaaaaat atcagtttaa taataatgaa tctattttat
      301 taattttgaa attaaattta aaataattaa tagagagggg aaatgaaatt aggatggaaa
      361 agaaaaaaat aggcctatgg agtttggtat ttatgaatgt atcagcttta tatggtataa
      421 gatggatagc taagtctaca gcatcaagct ttggtttagg attaggtgca atacctatgt
      481 ggttattatt ctcatttata tactttgtac caggagcttt aatttgcgct gaattagctt
      541 ctacatatca aaagggtgat ggaggtcttt acgattgggt taaggaagcc tttggagaaa
      601 agtatggatt tttagtttct tggttaaact ggacagctaa aatattttgg tattcttcat
      661 ttttaacttt cttagcagta aatgtttctt atacaatagg aaatccagct cttgctgata
      721 ataagatgtt tgtacttata ttatctttaa taatattctg ggcattatct cttatagcaa
      781 ctaaaggaat aagttttaca aaaatattta ctaatacagg tgcattaggt tcaacaatac
      841 caagtgctat acttataata ttttcatttg tagcagtatt tatgttgaaa aaacatgata
      901 tagcttctac atatacaata gcttcaatga ctccaaaact taatatggat gcatttgttg
      961 ctatatcagc tataatgttt ggtttagcag gagcagagac tgctgctaac ttcataacag
     1021 aaatagataa gcctgaaaag aacttcccta aggcaatatt aatatcagct ggaatagtag
     1081 cttcattata tgtattaggt tcaattgcaa taacaatgat aataccacca gaccaaatta
     1141 ctgcttctaa aggtgtatta gatgcacttt ctgctgtttg tacgtcttta ggaattggtt
     1201 cttggttggt tcaattaata gcttttggta tagctttctc agtattagga gcaatagttc
     1261 tttatatagc atcaccaata aaaatgttat ttggtagtgt taaaaaagga atatttccag
     1321 atagtttggt agaggtaaat gaacacaaga taccttctaa agcagttata cttcaagcaa
     1381 taatagttac tataatacta ttggtgacta cattaatgcc ttctgttgat gctatataca
     1441 atgttcttgt aactatgaca gcattaactg cattattccc atatgtatta ttatatgctt
     1501 cttatattaa actaagaaaa gagagaccag atgaaattag accttatact atggcaaaat
     1561 caacaagtac ttgcctaggt ttagcaaaaa tggttttagt tgtaactgta gttggtatat
     1621 tattatctgc agcaccagtt atgccgacat ttgctgaaaa tgttatatat gagatagaga
     1681 tgattggtgg aggtcttcta gttatattat ctggtctatg gttatggaat agatatgaaa
     1741 agaaaacagc taaataaaat gtattttaaa aatttctttt ggatattata atatatagtt
     1801 gacaatttat agtgtaagga atattataaa ataaagaaat taataaatta aatcctgaga
     1861 taagaaatag taaataaata tatttttgca cagagaatta aggatgctga gaacttaata
     1921 aaaagtattt attgaatggg tctttgagga gtaaaatgaa atgtaattta agattacaga
     1981 gtatttgata tcgtgtatgc acgttatagc atatattaag atatatatat tcagttttga
     2041 gtgtatataa atttaggtgg taccacggaa aatatatccg tcctattgat ttaggacgga
     2101 ttttttaata tagaaaattt aggaggaaaa atgaacgtaa taggaattat aggtgcaatg
     2161 gatgaagaag ttagtatttt agttgattta atggatatta gagagactat aaaaaaagct
     2221 agcttagaat tttataaggg aattttgaaa ggtaagaatg tagtgttagt aaagtgtggt
     2281 ataggtaagg ttaactctgc tctttgtgcc cagatactaa taagcgaatt taaggttgat
     2341 gctattgtta atacaggtgt tgcaggagct ttaaatgaaa aacttgatgt taatgatata
     2401 gtaatatcaa ctgatgctat acagtatgat gtagatacta cagcatttgg agaccctaag
     2461 ggagttatac caagaatgaa gacttctgta tttaaagcag atgaaagact tatagatgct
     2521 gcttacaaat cttctgtgga agaagtaaag actcataaag tattaaaagg aagagtagtt
     2581 acaggagata aatttataaa ttctaaagaa ttaaaagaag aattagtaaa tgattttggt
     2641 ggatattgtg gagaaatgga aggtggagct atagctcatg tatgctattt aaataatact
     2701 ccattcgtaa taataagagc aatgtctgat aaggcagatg gaagtgctga tgttacttat
     2761 gatgtatttg tacatgatgc tgccaataac tctaaagaca tagttttaaa tatgttaaaa
     2821 tcaatataaa tatataagga ggtacaaaaa aatgagtgaa aaaaaagtta tatttagtgg
     2881 agcacagcca tcaggcaaaa tgactttagg aaattattta ggagctataa aaaactggac
     2941 tgaacttcaa gataattatg attgctatta tagtatagtt gatttacatg caataacagt
     3001 acctcaggac cctaaagttt taagagcgaa tgctatagaa ttacttgctc aatatatagc
     3061 ttgtggatta gacccagaaa aaaatactat atttatacaa tcacatgtca aagagcatgt
     3121 tgaacttatg tgggtattga atactatgac ttatatgggt gaattaagta gaatgactca
     3181 attcaaagat aaatcacaaa agagtgaagc aaatctaaat gcaggtttat tcacatatcc
     3241 agttcttatg gccgctgaca tattgttgta tcaaactgac ctagttccag ttggagatga
     3301 ccaaaaacaa catttagaat tagctcgtga tttagcaaat aggtttaata atagattttc
     3361 acctactttt gtagttccag aaggatacta cccaaaaggt ggagcaagag tgatgagttt
     3421 gcaagagcca actaaaaaaa tgagtaaatc cgattcaaat gaaaatgcat ttatactatt
     3481 agcagatgat tcagattcta taaagagaaa gataaaaaga agtgttactg attctttagg
     3541 tgttgtaaaa tacaatgatg aacaaccagg tattaaaaat ctaattgata tatattctaa
     3601 tttaagtaaa aagagtgtag aagagattgt aaacatgtat gaaggaaagg gttatggcat
     3661 atttaaagaa gatgtggctg aagtagtatc agaagcttta agacctataa gagaaaaata
     3721 tgttgacctt cttaataata aagattattt agaaaaaata tatagtatgg gagcagaaaa
     3781 agccgaaaaa caagctagaa aaactcttag aaaagtttat aaaaaagttg gatttataga
     3841 gagaagatac taattattag ctatctttac ttgataaata aattttaaag ttattaaaaa
     3901 ggtatatgat atgaaagaga gagtctcaaa gtgaactttt gttcatgaga cactcttttt
     3961 tgtatgaaat aaaatatatt ttaatcactt caacaatgaa aaagaggatt atctctattt
     4021 tgagacagca tcttttgata ataaaaaaag atgagctttt gctcatctca aaggggttag
     4081 attattctta ctttggggaa agtagaataa aggtaaaagt taactgatgt caactttgtc
     4141 ttttttgcat atcactactg agcataaaaa aatcagaaaa ccatagcctt ctgaaacaac
     4201 ttctaacata cattgtattt tatacccaat atgtaattta tgagttaatt ttaactctta
     4261 taatggaaat tgtcaataat atatcaaaaa aatatttttt aaatttatat aattaaatga
     4321 atttatgttt atatataggt gatatgatac agataattat ctttataata caatatagtt
     4381 ctaaaaaaga atatgttaga agaagagaat atttaaataa aaaatacctt ataaagttaa
     4441 ttttttagta ctttataagg taaatatata ttgtattgtg gggacaaatt taaatcattt
     4501 gatactaatc aacactaaga actaataact ctctatcgtc tggagtaatt gcatatattc
     4561 ttaatttctt gtatgtcata atattttctc cacctgtaaa ataactccta agattaacat
     4621 aattagtttt aggctgattt ggatctccta aataattacg catttcttta tctttagttc
     4681 caatatcttt aaatccagaa tattgtaaag tagcttcaac tctatatttt tggatataat
     4741 cgagagcttc tttattagtt tgtgttggtg caaaatacat accatttata taagtatttc
     4801 ctgtacttgg attaaaattt aaatctgcaa aatatatttt atgtgcatcc attatttctt
     4861 ggtcagttgg aattttaact tctggttcat caagtatttc aggagtacta tttagctctg
     4921 taatagataa gttatctaag tatgttgtac cactaccaat taatgttatc tctatattag
     4981 aagaatcttt ttcagtagtt tcaaattcat aactaaattt tgtatatcct tgttctggta
     5041 ccaaataatc cgttttctct tcttttgctt ttatctttac aattattgaa ttagatgtta
     5101 aaggatcctt tgaatatcca ctaaaaacat aacgtttata aggttttagc tcagacatag
     5161 ggattttaat cttcgtctca ccatttaatt tatttgctga gccttgtaaa ccatcttgat
     5221 tcgtagtatt gacattactc catgtactag ggtaattatt ataatcatca aaattagtaa
     5281 agtaagttgg tgtttttata agtatattca ttcctctttc aagtttaaca ttatatatct
     5341 ttttatcaga caaagttttt aaactatctt taatcttatt ggctgtatta tcatcaaata
     5401 tgagttcaac acaactttca tctattggta tatcattaaa atataacaaa ccatcttttt
     5461 tagtagcgcc aaaagctttt tcaattgctt ctccaattgt aagttcaggt gttttatctt
     5521 ctggatcctg taaattttta gcagtaactc ttctttcgta agattcattc tctgtatcta
     5581 atataataga tgcagaaata ctgtcaattt gacttatata gtctgaccaa ctatttcctt
     5641 ctgttactat ttgtccagaa ctattttttg taccaaaatt tccacttact tgtgttgttt
     5701 ctaatttaat ttgctttcca gcatctaatt tttttaattg atcataattt ataggaatca
     5761 gtctagagct aaattgatcc attgtgttaa gagctagagg tgaaagccct tttttgggat
     5821 aagtatctcc aggagataga ttattgccaa tttgattttc ttgtgctttg atagttgata
     5881 atgtatctcc atctaacact aaatttgttg ttggtgtcac tttgtacata ggtgcagtac
     5941 ctgtgttgta atatctaaca tttgcattta tatatgcaga ttctcctttg tttatactta
     6001 atccagtatt ccatgattct ccattactat cttgaacagc agttgaatta tctgttgtat
     6061 gggaataatt tgtagttaca ttagctgtga atccattttg atatcctaca ttaacagaca
     6121 caccagctgt attagattca gttttactgt tagtagtagc tctggaaaca gttttacctt
     6181 gatcagtaga ggcatgttca tttgtagata taattaattt ttccatacct actccaacaa
     6241 ttggatatgc tgcaactaac ggatctcttg cttcagtctt tatagccttg tcaaaagaac
     6301 ctgaagcttt ttcataatct gtatatggat ctccagcagt atttgactct aaataatttg
     6361 atacatattt cttatagcct tgttctgcaa aactatcttc ccacttaact gcaattaagt
     6421 ccttaatagt atatccattt cgttcatatg aatctggtat attatcatta tctgtatcca
     6481 aatcttcgtc ttcccaatca gacatcaact ttgggtcaaa gaaattgtta tttgggataa
     6541 atggatcatc tttttctata ttagaataat ctcttaagaa taaattttct tctggtataa
     6601 ttttcttcat accatctaat tcccaataaa gattaggtga tgataaatta tctattgaac
     6661 ctaaattttt atcttgtagc tctattctaa ctttatattc tttacccttt ttcatattaa
     6721 ctttaagtgt atttgatata gtactctcag tatttacttg cattaagaca tcatctctat
     6781 cagttgataa tgtatattca ccatccttag aaggaattat tcttcctgtc catcgtatag
     6841 attttacatc tgatttgtct ttatccagaa gtttatctac tttcttttct tcaaatttta
     6901 aattaccatc ttttatgggt gccattaatt ttaaatcttt aaagtgctca tctgtgaaat
     6961 aatatcccat taatccattg tttgggagta tatcttcatt tacaatttct tttttcttat
     7021 tagaatgatt acttgtacta gtttgagcat atacaggata tactagtgct tgactaacta
     7081 tagctgtaag tgttaaaaaa cttaatacct ttttattcct catttgtatt ttcattgttt
     7141 ctcctcctta aatttgaaaa atattaaatt atttatgtga aaaaatatta aggtatcaat
     7201 gttgcatcaa caattaattt tgttatagta ccatctttgt aagaatcaat tttattgatt
     7261 ttaaatttgc ttccatgatt taaaagcact tcatattcac ctgcataacc tggaatagct
     7321 gatagataag ctccaggaga acctttaggt atagttatac gtagtactat ttttctttta
     7381 gcaaatgcac tcatattcac actaccaata ctagtactaa taaagtttgg ataagacagt
     7441 gcttgtcctt cccattttga tttaaaagca tctatatttt ctagtttgtt aaaatcatat
     7501 tcaggggaag taagagttaa accaaattct tgaggaccag atcttctata tacagttaaa
     7561 ttagttggaa taggttcacg ttttaatgca ttttcaatgt ttgtgatttt agaatctaat
     7621 tctgggttag gattatttac tggaccattt gatattaaat aattattaat tgcagtatat
     7681 cctccacgca tataatcatt tacatcagca agttcattag gtgttaattt attactccaa
     7741 tcattataat ttgctttacc ccaagaatcc cccttactta catcatcttt aaaatcaaga
     7801 ctacttacaa cagatgcttc tgctttaata tagtgcttcc catctataac tatacgaaca
     7861 attttatcta tttttatact atatccttgc tctattaatg tacttacatt gttagtattt
     7921 gtatatggta acataccagt atttctaggt aattttaagt gcataagtaa tggtgtaggt
     7981 ttttcatcac cttttccagg ttcatataaa gaaatatctt taaatccatc ttgcttaaat
     8041 aatttgtttt gtatagtttc tttaaactca ttaaattttt ctaatgaaat ttcgttttga
     8101 ttttctgttc ttattacttt attaaatgca aatttttctg gagattcaaa ataatagaca
     8161 tacataggtt tatctatttt attttttgat atagcatttc gaagttcttt atattctttt
     8221 tctcgagaat ttgcttctat ttgataatca taaaaataat ttcttgtctg agaatattta
     8281 cttatttcta cagaatcttt tttatatgat tctaatgctt ctttttcaga tctttcaagt
     8341 ttttgctcta ttctttctgc ttcttttctt tcccattctt tagccttttc tttatcttta
     8401 agaaaatctt ctggtctttc tataggagcc ttgtaagtag tattgcaaac tttttcacta
     8461 tagctttgta agtcttgtgc ataaatgtta ttaggtaaca aaccacctaa agttagatat
     8521 aatatcaaca atatagatat acaattacta atccttttat gtttcctaaa ttttttcatt
     8581 tattctccct cccaatatta gtttaattaa ctcttgaact ttaatttatt tagattatat
     8641 aatctaaata agaatttttt atataaattt cataaaatat gtaactatat atcataaaaa
     8701 tatgtattta tatgatatgt cagtacaaaa taacaactga atattttaaa gagactattt
     8761 catact
//
LOCUS       DAEPLF010000146         8722 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEPLF010000146 DAEPLF010000000
VERSION     DAEPLF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.146"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(36..3143)
                     /locus_tag="KR314_002689"
     CDS             complement(36..3143)
                     /locus_tag="KR314_002689"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBH0329059.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(3236..3468)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3643..4170)
                     /locus_tag="KR314_002690"
     CDS             complement(3643..4170)
                     /locus_tag="KR314_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBH0329060.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(4205..4990)
                     /locus_tag="KR314_002691"
     CDS             complement(4205..4990)
                     /locus_tag="KR314_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBH0329061.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(5000..5257)
                     /locus_tag="KR314_002692"
     CDS             complement(5000..5257)
                     /locus_tag="KR314_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBH0329062.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFNILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(5276..5734)
                     /locus_tag="KR314_002693"
     CDS             complement(5276..5734)
                     /locus_tag="KR314_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBH0329063.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(5774..6478)
                     /locus_tag="KR314_002694"
     CDS             complement(5774..6478)
                     /locus_tag="KR314_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBH0329064.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(6488..7123)
                     /locus_tag="KR314_002695"
     CDS             complement(6488..7123)
                     /locus_tag="KR314_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329065.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IPVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            7504..8544
                     /locus_tag="KR314_002696"
     CDS             7504..8544
                     /locus_tag="KR314_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0329066.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGIITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
BASE COUNT         2598 a         1548 c          995 g         3581 t
ORIGIN      
        1 tttctatagg gattttggtt aactaactat tctttttatt tttttataac ttttatacct
       61 gtcatatggt catttaagtt ttgttcctct aaagatttat catctactct gtttatttct
      121 aaagctaaag tttcagactt gatatattca ttaaaatggt caacagcctt agcaatttca
      181 tcatcaccac agtattctat tactatatta tctaatactt caaagttatt agattttctt
      241 aattgctgta ctttagatat aaattctcta gcataacctt cattaatcaa ctcttcagtt
      301 agcttagtat ctagtataac aaaaagattg ttttcaacag atacattaaa tccttcctta
      361 gcagttatac ctattaatac taaatcctta ttaaattcaa aagcttcgcc atctaaatca
      421 actgttaaag tctcaccttt ttcaagttta ttagcagttt catgagcatt taatttactt
      481 aatgctccaa cgaataaatt catcttagaa cctaataatg gccctgcttc cttaaagttt
      541 ggttttaaac taaagttcat atactcatct aaatccttag caaatattac ctctttaaca
      601 ttaagctctt cttttattaa atcaactaca tcacttattg tatcttcaaa cttaccatct
      661 actaacactt tttgtaatgg ttgacgaact tttattcttt ctacttctct tgaagctcta
      721 cctaaagtaa ctaagttttt agctaaatcc attttttctt ctaatttagt atctactaac
      781 tcttcattac atttaggata ttcagctaaa tgaacagata cttctcctgt taagtttcta
      841 tacatttctt ctgacatata tggtgcaaat ggagctatag ctctacaaag accatgtaac
      901 aattcataag ttgtattgta cactgctttt ttatcttctg ttaattcagt tgcccagaat
      961 cttcttcttg aacgtcttat ataccagtta gataaatcat catttataaa atcttgtatc
     1021 atccttaaag ttttatttaa ctcaaatata gctaaatttt cttctacatc tttcattaaa
     1081 ttattgaact tagataatat ccatctatca agttctggtc tatctttata ttctacaaaa
     1141 aattcagttg gatttatatc atctgtgttt gcatataaag taaagaagtt atatacattc
     1201 ttcatagtac ctataaattt actttggatt tcttttaatc catccatatc aaatttagtt
     1261 ggagtccatg gtggagaaac atatagtaaa taccatctta atgcatctgc tccatattgg
     1321 tcaaacaatt ccattggatt tactgtattt cctcttgatt tactcatttt cttaccatct
     1381 ttatcaagta caaggtctgg aactaaaaca ctcttatatg gtgcttttcc taatacaaat
     1441 gttgatactg caagtaatga gtagaaccaa cctctagtct ggtctatacc ttctgatata
     1501 tagtcagctg ggaacaattg gtcaaaattt tccttatttt caaatggata gtggtgttga
     1561 gcaaatggca tagccccact atcaaaccaa cagtctataa cctctgttac tcttgtcatt
     1621 ggttttccac atttttcaca ttttaagtgt atatcatcta catatggtct atgaagttca
     1681 actgtttctg gatttacatc ttctatagct ttttcagcta attcagctct tgaaccaact
     1741 gaatctgtat gaccacattc acatctccat ataggaagtg gagttcccca atatctagtt
     1801 cttgatattg cccagtcatt tagattttct aaccagtttc caaatctctt ctctcctaca
     1861 aaacttggga accattttac accattatta ttttctatta atttatcttt aagtttagtc
     1921 atctctatat accagcttgg ttttgcatag tatactaatg gagtttgaca tctccagcag
     1981 tgtgggtaat tatgaactac tttttctttg ctatataatt tattttcaga agctagccat
     2041 tttattattt caacatctag cccatcttcc attacaaatc taccttccca aggagtagat
     2101 gtaaatttac cagtttcatc tactggttga ataactggta aatcatatct tttacctgtg
     2161 ttgtagtcat cttcaccaaa tgctggtgct gtatgaacta taccagttcc atcttcagta
     2221 gttacataat ctcctactgt aataaaaaat gcttttttgt caacttctac aaaaggcatt
     2281 aattgttcat attctaaacc ttctaggtct ttacctttca tttcttctaa aacttcataa
     2341 tcttcaccta gtactttatc agctaaaggt ttagatacat aatatacctc atcacctttt
     2401 ttaactttta aataatctac atcagcattt actgttaatg aaacatttga tggtaaagtc
     2461 catggagttg ttgtccatgc taagaagtat tcatcagcat cttttttctt aaatttagct
     2521 ataactgtat tagtttttac ttctttatat ccttgtgcaa cctcatgact agcaagacca
     2581 gttccacatc ttgggcaata aggaagtatt ttatgccctt catatatata tccttctttg
     2641 ttgaatttgt ttagtatcca ccatacagac tctatatagt tattatctaa agttatatat
     2701 ggattatcta aatcaacttc ataagccatt ctctcagtca ttattctcca ttgtttttca
     2761 aatgagaaaa ctgactctct acatttttgg ttaaattcag ctattccata ttcttctatt
     2821 tgttgcttgc tagtaagacc taactctttc tctacttgta tttcaactgg taatccatgt
     2881 gtatcccaac cagcttttct ctttacttga taaccagaca ttgttttata tctacaaact
     2941 gaatctttaa gcgttctaga taaaacatgg tgtactccag gatttccatt agctgttgga
     3001 ggtccttcat agaatacgaa acttgggtca tctttccctt tttctaaagt tttttcaagt
     3061 atatttatgt ctttccaata atcaaaaact tgtgcttctg cttgttttac agaactgtct
     3121 accaatggct taaatttcgc catgtaaatc accctttcct actaaatatt ataattccaa
     3181 aattttttat tctttcacat aaatttaaat actaaaaaac tcgccccctt taaaccaaag
     3241 ggacgagtta taaatcgcgt taccacccta attctatatg taaactaaat actagctaca
     3301 tataacactc taatagtata acggtactaa ccggcacagc ttagtagaat ttaatcgttc
     3361 agcctgcagc tcaagagtga tttttatctt tataaattca ttggttctca gcatacccaa
     3421 ttctctgtaa aattatctaa agataaatgt ctctatcaac gcttttaaaa atttatctgt
     3481 gtttatatga aattttataa caaataattt gaatagtcaa tatatttttg aaaaatataa
     3541 taatttctat ttttcttaca tttttatcct atttttaatc tttgttatag atttctcaaa
     3601 ctcacacagt tattctacat aaaaaataaa caaattacaa gcttattcta aagttgtagc
     3661 agcttcatca ttactataat tatacactgt tgttccttca aatgcattta cacatttttc
     3721 atctacatca taaaaaatct catcaacact tcttatttca tcttctaaca gtgatttaaa
     3781 tttatttcta aatatcttaa attctttgac taaagcatca tactcttttc ttatctctat
     3841 gacacggtta ttagcttgtt ctataatttg tctagctttt aaatcagctt cttcaacaat
     3901 tatcttagcc tttttattag ccgcgctaca agtgtcttct gctgcacttt gagcagttat
     3961 taaagtagct tttagtgttt cttcaatatt ttcatacttg tttacttggt cttgatataa
     4021 ccctaatttt tctttaagag ctgtattttc cctacataga gattcataat cttccttgat
     4081 gatatctaaa aattcatcaa cctcttcatc tctataccct cttaaacctt ttttaaactc
     4141 tttattttct atctcaattg gagttagcat tacatttcct cccagctata atttaaatta
     4201 gtccttatac aattaatctt gcttgaactt ttatttttcc ttttttggta atatctccta
     4261 tttgtgttac cttagctctt cctttgcctc taatagaaat caaatcatca ttttttacta
     4321 ttttagaagt tgaagttatc ttttcataat tgacatggac tttttctcca tttatgtatt
     4381 ttgcactatc ttgtctagat atattgtaaa taccacttat cacacaatct aacctatctg
     4441 aagatactgt aaatgatact tctttatatt ttgaagaact attaacaata ttttctctgg
     4501 aaatttcctt tacaattaca ttatttcttg aaaccttttc taagtttaca attataaaat
     4561 cgcaaatatc actacttaca actacttgac aaaagctatc atgtataatt atatctccaa
     4621 ttttttctct ttttatacct agacccagta ttgaccctaa gtaatcttta tgagatatat
     4681 ttttaaactt gaaattccct tctatttgta aaaatctaag agtatcttct atatcttcat
     4741 attccatgta aaatggataa ataaatacag tgcttctttc agcttgttca taacctccat
     4801 ccacactgta ttttatttca tttgtagaat ttaatattgc tacagcattt ttcacttcat
     4861 atggattaag aaactccgta gacttaacat catagttttt tatacaacta ttagccttgt
     4921 ctatcacttt aaacatttta tttttcaagt ctatatcctt tatgtgattt gttagtttta
     4981 atttatccat cctatcacct tagaatataa ccaatactaa ttgtcttagg aacatcaata
     5041 atactattgc aattaaagga gaaaaatcta taggtccatt tgtatattta tacattatag
     5101 accttattgg cccttctatt ggctctgtaa gattttccaa aatattaaaa attttacttt
     5161 gtcttccatt tggaaaccaa gtcatcaagc tttttataac tatcatccat gctattatat
     5221 ctaaaagata ataaagtgca attcttattg ttcccatata ttaaccgcct ttttactatt
     5281 tattttgcca agggaagaat gctttacttt ctaattcttt tttcatattt gcatctatat
     5341 ccacattatt aggagctagt ataaatattg atttagaaac cttttgtata cttccatcta
     5401 atacataaac tgccccattc ataaagttaa atatagattt acgaacttca gaattagtca
     5461 atccttccaa attcactata actgcccttc tttgttttag atggtctgct atagttgcag
     5521 cttcatcata aaccttaggt tcaactatta caactttcat ttggctagtt gtatgtaaat
     5581 ttacaatttt atttgctttt gcagtactaa agccactgtt catttcttct tcttgaacta
     5641 tatcgtccat gccactttca tattcatctt caatataatc ttcttcttca tcaactatcc
     5701 aattcttaaa tttagatatt attccatttg acattattaa cttcctcctt aaaatttatt
     5761 caaatttgag taattatttt ttattgtagt ttctttctcc aaagattgat gttccaactc
     5821 taacaatggt agccccttcc tctatagcaa tttcataatc gtgactcatt cccattgaca
     5881 aaacatccat ccctacatta gggatgttta gttcttttat ttgttttgat aaatctttaa
     5941 gacctttaaa tacacctctt atctcatctt catcttctat aaaaggtgcc attgtcatta
     6001 gaccctttat ttttatattt ttatattttt ctgacacttc tttgacaaaa tctacaactt
     6061 cttctctatc aagtccatgc ttagactctt ctttagatat attcacttgt actaagcaat
     6121 ctatttctct gtttatttgt tctgcacgtt tttgaatttc ttcgcataat gcaattctat
     6181 ctaaagagtg tatcatacaa actttatcga ttatgtactt aactttattg gtttgcaaag
     6241 ttcctatcag gtgccagttt aatttgtcac ctataatatc atactttcta gctaattctt
     6301 ggggtttatt ttcaccaaca gaagtgattc cttccctcat tgcctcatta actaagtcta
     6361 catcaacagt ctttgtgact gccaaaaggg taatctcttt gctgtctcta tctacttttt
     6421 ttgcagcttt atcaattttg tttcttatct cagacaaatt atctttaata gaacccatta
     6481 ttatcaccta ctttatcctt atatttgtgt ttatactgtt aggtcttaaa attattttgt
     6541 catatagatt aacacttgta actccattta tgtcattttt ataataatcc acaaatacag
     6601 aagattcatt ctcataagtt attccttcta atatgacaaa ttttacactt tgtgattgtt
     6661 catcaattac atatacacca cgtttattgt cttttgtttt tatagaagtt ttaggtattt
     6721 ttaaacattc catctgatta tatattatat caaattcttc gacacttgta tcctgatttg
     6781 caacaatttg gttacttatt tttaatttta ccattatttt attattattt tcatatattt
     6841 tgtaaacttt tgcatctaat ttttgctcat gatattcaat tattacattt tgattttttt
     6901 caaagttatt agaatcttct ttactgatgt atgtagatag ataattactt tggtcattgg
     6961 catctttttc tatagttcct tttacaggaa tctttgcatc aactttttca ttttttaaca
     7021 ccaacgtatc tacatttttg ctaattatca ttactaatat attaaatact aaatatataa
     7081 gaataccaag tatcactaat ctggtatatt ttaccttttt caaagactct ccccctataa
     7141 aattactttc attataagta cttcttctaa aaaatgttta ttcctttttt ataagctaat
     7201 attttacgtt ctaataatta ttttttccat attaaattta ttttttatca atcttcctat
     7261 acaagactgc attatttata tataattgta ttataaagta taagtttaat ttacatttta
     7321 tacattaagc ttactttata tgtttttata gttattttta tttttagatg caatataaaa
     7381 ccaattaaga tttaaaataa cttttgttat gttgacattt atttaggtaa tcatttacga
     7441 atcaggataa cgttatcata acaaaagttt atgtcctaaa aaaaatacaa ggaggatttt
     7501 cttatgtcag aaattttatt aggaacaggt ttacttttat tggttctatg ttgtttcaca
     7561 atttttagtt acaaggcccc atatggtatg aaggctatgg gagctctagc aaacgctgca
     7621 tgtgctactt tcttagtaga ggcattcaac ctttctttac ttggtgatgt tttaggtatg
     7681 gaatttttta gaagtgttgg agctgctaat ggtagtcttg gtggagttgc tgctgctata
     7741 cttgtgcctt tagccttagg tgtatctcca gtttatgcag ttttaatagg attatcttgt
     7801 ttagattttt ctatattatc tggattcgtt gctggatact ttgtttcttt cttaattaag
     7861 tttatagaga aaaaagtgcc acctggagtg gatttagtgg ttgtaattct tctatgtgca
     7921 ccgctatctc gtcttatagc atcaaatgta gacccattag ttaatagtac actactacaa
     7981 attggacaag ttttgataga agctaccaac attagcccta taataatggg aattatacta
     8041 ggaggcataa taacagtagt tggaacgtct ccacttagtt caatggctct tacatctata
     8101 attggtctta ctggtcttcc tatggcaata ggagcattag gtgcttttgg ttcagctttt
     8161 atgaactcaa cactatttgc aaaattgaaa ttaggttcta aaaaagatat tatatctgtt
     8221 gctattgaac cattaaccca atcagacata atttcagcta atccaatacc tatatattct
     8281 actaatttct ttggaggagc cttttctggt atcattgtag ccttgatggg tcttgtaaac
     8341 atgacaccag gtacagctac acctgtggct ggttttgcta taatgtttgc ttataatcca
     8401 gttcaacaag ttcttataac agctggttta tgtgctttat tcaatgtaat tgcaggattt
     8461 ttaggttcaa aaatattcag tacctttaaa gttcttactg ctggtgaaat acgtggtact
     8521 gatgtagaag aagaaattgc ttaaaaaaca ataagattta taaatttata tctcaagtag
     8581 tagagtttta tattgtaggc aattttatta attgcaaacg ttttattttt tatttatgtt
     8641 tatgattatt atgtgtgttg aatatttaga taaattttta tttattattt ttatttagat
     8701 ttttgtttag ttttttattt gg
//
LOCUS       DAEPLF010000147         8553 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEPLF010000147 DAEPLF010000000
VERSION     DAEPLF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8553
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.147"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(18..1622)
                     /gene="bclA2"
                     /locus_tag="KR314_002697"
     CDS             complement(18..1622)
                     /gene="bclA2"
                     /locus_tag="KR314_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBH0329067.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGPTGPTGNTGATGANGITGPTGATGATGA
                     NGITGPTGNKGATGANGITGPTGATGATGANGITGPTGNTGATGANGATGLTGATGAT
                     GANGITGPTGATGATGANGVTGATGPTGNTGATGPTGSIGATGANGVTGATGPIGATG
                     PTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGLVGPTGATGAVGA
                     TGPTGADGAVGPTGATGATGANGATGPTGAVGATGANGVAGPIGPTGPTGANGVAGAT
                     GATGATGANGATGPTGAVGATGANGVAGPIGPTGPTGANGTTGATGATGATGANGATG
                     PTGATGATGVLAANNAQFTVSSSSLGNNTLVTFNSSFINGTNITFPTSSTINLAVGGI
                     YNVSFGIRAILSLAGFMSITTNFNGVAQNNFIAKAVNTLTSSDVSVSLSFLVDARAAA
                     VTLSFTFGSGTTGTSPAGYVSVYRIQ"
     gene            complement(2000..2539)
                     /gene="hpt"
                     /locus_tag="KR314_002698"
     CDS             complement(2000..2539)
                     /gene="hpt"
                     /locus_tag="KR314_002698"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBH0329068.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(2867..4174)
                     /locus_tag="KR314_002699"
     CDS             complement(2867..4174)
                     /locus_tag="KR314_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBH0329069.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDEKLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4599..6374)
                     /locus_tag="KR314_002700"
     CDS             complement(4599..6374)
                     /locus_tag="KR314_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0329070.1"
                     /translation="MMVMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDVCRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6371..6508)
                     /locus_tag="KR314_002701"
     CDS             complement(6371..6508)
                     /locus_tag="KR314_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329071.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6586..7155)
                     /locus_tag="KR314_002702"
     CDS             complement(6586..7155)
                     /locus_tag="KR314_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH0329072.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7339..7974)
                     /locus_tag="KR314_002703"
     CDS             complement(7339..7974)
                     /locus_tag="KR314_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH0329073.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2482 a         1675 c          970 g         3426 t
ORIGIN      
        1 tcttttcatc atataaacta ttgtattcta taaactgata catacccagc tggagaagta
       61 cctgtcgtgc ctgaaccaaa tgtaaagctt aaagtaacag ctgctgctct agcatcaact
      121 aaaaagctta aacttacact tacatctgat gaagtaagcg tatttactgc ttttgcaata
      181 aagttatttt gggctactcc attaaagtta gtagtaattg acataaatcc tgcaagtgaa
      241 agtatggcac gtataccgaa agatacattg tatatccctc caactgcaag atttatagta
      301 ctacttgttg gaaaagttat attagttcca tttataaatg atgaattaaa tgtcactaat
      361 gtattattcc ctaaacttga agaagatact gtaaattgtg cattgtttgc tgctaacact
      421 cctgttgctc cggtcgctcc tgttggacct gttgctccat ttgctcccgt tgctccggtc
      481 gcccctgttg ctccggtcgt tccatttgct cctgttggtc ctgttggacc tattggacct
      541 gctactccat ttgctcccgt tgctcctact gctcctgttg ggcctgttgc tccatttgcc
      601 cctgttgctc cggtcgctcc tgttgctcct gctactccat ttgctccggt tggacctgtt
      661 ggacctattg gacctgctac tccattcgct ccagttgctc ctactgctcc cgttgggcct
      721 gttgctccat ttgcccctgt tgctccggtt gctccggttg gacctactgc tccatctgct
      781 cccgttgggc ctgtcgctcc tactgctccg gtcgctcctg ttggacctac caaaccattt
      841 gctccggttg ctccggttgg gcctgttgga cctaccaaac catttgctcc ggttgctccg
      901 gttgggcctg ttgggcctgt tggacctacc aaaccatctg gacctgttgc tcctactgct
      961 ccggttggac ctgttgctcc tattggacct gtggcacctg ttactccatt tgctcccgtt
     1021 gctcctatac ttccggttgg acctgttgct cctgtatttc ctgttgggcc tgtagcacct
     1081 gttactccat ttgctcccgt tgctcctgtt gctcctgttg gacctgttat cccattcgct
     1141 cccgttgccc cagttgctcc ggttagtccg gttgcaccat ttgctcccgt tgctcctgta
     1201 tttcctgttg gacctgttat tccatttgct cccgttgctc ctgttgctcc tgttggacct
     1261 gttatcccat tcgctcccgt tgctcccttg tttccggttg gacctgttat tccatttgct
     1321 cccgttgctc ctgttgctcc tgttggacct gttattccat ttgctccagt cgctcctgta
     1381 tttcctgttg gtcctgttgg acctgttgtt ccatttgctc cagtcgctcc catatttcct
     1441 gttggtcctg ttattccatt cgccccggtt gctcccgtat ttcctgttgg tcctgttatt
     1501 ccatttgctc cggtcgctcc ggttgcgcct ctaggccccg tcggtcctgt tggaccagta
     1561 gcacctgttg gccctgttgg acctacatct tgatataaac ttggacctga aatatcactc
     1621 atataattaa tcctcctttt ttaaagttag agtattactc tataaatatt gagtattact
     1681 ctatataatt atatgaatcg acaatttctt atgtgataat gtatctattt gcttatatca
     1741 acatatatgc tttgattctt aaatttcatt acttttttgt aaaatctttt acaattatta
     1801 gtactctttt acaattatta atacaattaa aaaactcatt tcccatcaat tatttaaaat
     1861 ttttttatta tgttataaaa aatagataat taaattgcat aaaattaatt taatactact
     1921 ttaattactt tttaaataaa aaaacctcaa cacgattaat aaaaatcata ttaaggcatc
     1981 cacaaaatta tataattgct tatctatctt catcagtaac attgaatata taaggaacat
     2041 ttctataata atcaccaaaa tcaaacccat atccaaccac aaacttatct tctatttcaa
     2101 atccacaata atctggcact aaatcaactt ttcttctgct tggtttatcc aaaagaacac
     2161 agcattttaa actagcagga tttttagctt ttagatgttt taacacaaaa tccatagtaa
     2221 gtgcagaatc agtaatatcg tctacaacca aaacatcata tcctgctata tcagtagtca
     2281 catctgaaac cactttaacc tttccagtac tttcttcatt gtttccataa ctagatgtag
     2341 tcataaaatt aactctaagt ggtaaattta tatttctaac taagtctgca caaaatataa
     2401 agctaccttt taatagagaa actaccataa gatttttatc tttataatct ttttctataa
     2461 ttgctcctaa ttcccttaat ttatctttaa ttttttcttc tgaatacaat acttcccatt
     2521 tttttgtttc tatatccata aaaagacctc caattgtaat aatccttctt aattttataa
     2581 gagattgctc actttttcaa tactcttatt caatattagt caatattatt gagtttttat
     2641 gttcattcaa caattattaa caatgtatct tatattttaa gtagttcata tatccacact
     2701 aaattacata ctatatattt tagacaaaaa aagaaaaaac ttgcactaac aagttttttc
     2761 tattatacat aaatttatac ataatcatat tttattagag ggtactaatt aaatttattt
     2821 atgctatttt tgttatacct cataatacaa aacatattat aaagtactag tcaactttat
     2881 tcatatcatt cattacattt aagattatct catctgttat agacatacct ttgtcactta
     2941 ctctacctaa gttttgtata ctttgctcaa cacttgaacc aactatacca tttagtggtg
     3001 gtacaaaaca atcttgctta gctattatag cactttgtat agctgcagat gctgcagaag
     3061 ataattttaa tgcacaacca gctttcgcac cgtcacatat cattccactt aagtcagcta
     3121 tcatattttc tatagctcct tctatttgct tttcagtacc attcataagc caagagatac
     3181 cagcagttgc tccagtagaa gcagcaacac cacatccaca cacagcagat aatcttcctg
     3241 tatagttctt aatataacca gttactagat gagatatagc taaagccttt gctagttttt
     3301 catcagattg tgggaatttt ttattataag caacgattgg aagaattgct gttaatccat
     3361 gatttccact tccatttgaa ctcataacag gcatttttac gcctgccatt cttgcatctg
     3421 aagctccagc agttaacatc attgcataat ttataacatc attacctaaa agaccttctt
     3481 ctatagactt tttaattcca tatccgacac caataccagt tttttgtttt aatccatatt
     3541 cagccatctc ttcattcatt tttactccat ctagtaaaaa ttctatatct ttaaaatcaa
     3601 tttcttctac attttttatt aattcttgta tagttactgt atccattaaa ctttcagctt
     3661 tttctgccgc atcatttgaa gctgatactt ttggttcatt gtctaataat acttctccat
     3721 ctttttctaa gaacgtaaaa ttatcatgtt tagttcttat tctcacttta gcaatatgat
     3781 tttttccttt taacacaact tctataaata ctttctctct tgtatcagca ggtgttattg
     3841 atagtggagt attatccata taatcctcag ctattttaac ttcttctttt gttagagttt
     3901 ctaacacact taatccattt tcagaatggc cacctactat acctagagct gctgcaattt
     3961 ttaatcctaa tctttcagta cctggtatac ctacgcacat gccatttttg tagatactag
     4021 gactaactaa catacgattt tcaactattt cttctcctaa taattccttt gcctttgcac
     4081 atgctagagc taaggctact ggttcagtac accctactgc tggtttaact tctgccttta
     4141 acatttccac taattctttt cttatatctc tcattatata taaacctccc aattaagttt
     4201 agatttagta ctcaaataat taattaaata atttcttatc tatatttata gcaaaataca
     4261 tgccatattt tttatatctt tttatatttt tagtcttata ttctataacc attggtatga
     4321 ctgcttctat ttatgtattt tttttattta catagagtat ttatattctt taaaatggag
     4381 aaacatatga caatttcgca aaaagacaaa aaaaagtctc agaatgagat tctatatcat
     4441 tttgagacgg ctcaaatgtg ttatttttta acttataaaa accatttttt aattcataaa
     4501 aaaagattat gcaaaacact tatagtataa gtttaaagct ttctttaaag ttatgaattt
     4561 agaaaaattt aaattttata tagcttttta ttatattact atgaaattat attgtatttt
     4621 tcaatttttc tatataatgt agctcttgat attccaagtg ctttagctgt taattcttta
     4681 tcttttttat aatctttgta cttatttaaa gcttttatta tctcaatctt ctctagttca
     4741 tcaagtggaa ttatcctatc gcttttaact tctaactctt ctacagaggt attcttaatt
     4801 ttttgaggaa ttacattcat tgtgattatt gaagaagaag acatattcac actatattca
     4861 ataatattct gcaactctct tatattccct ggccacttat attccatcat agcatcaaca
     4921 accctgctat ctatgtcaat aacattttta tctaattttt ttgaatactc tttaaccata
     4981 tgcttgatta taagtgggat atcttctttt ctctctctta aacttggtaa ttttataggt
     5041 ataacattta atctataata taaatcttct ctaaaagtgc cattttgaac cattttttct
     5101 aaatctttat ttgtagcagc aataattctt acatctattg agacatttga tttacttcca
     5161 attttattaa gttccttttc ttgtaatact ctcaataact ttgactgtaa atgcaaactc
     5221 atatctccta tttcatctaa aaatatactt cccttatgag ctacttcaaa cattccaagc
     5281 tttccacctt ttttagctcc agtaaaagct ccctcttcat atccaaataa ttcactctct
     5341 aataaattgt ctggtatagc agcacagttt acagctataa aaggattatc tactctattg
     5401 ctatggttgt gtatggctct tgcaaatagc tccttacccg ttccactttc cccagtaata
     5461 agtactgttg atgttgattt agaagctatt agggcttcct ttttagaatt tcttatagct
     5521 acactttctc caattatgtt ttccaatttt attctataat ctttattcat cttattgtag
     5581 ttttttatag catctctttt atctataaaa tcaataacat atccctttaa ctcatttttt
     5641 aatactattt tattgatgtt gtatattccc ttcgaatcat atccgtatct tttataaaaa
     5701 aaactacacg atttgtcttt cttaaagtta tttatagaag gtttctttat aaaatctaat
     5761 tcgacaaata tgtttttgcc agttatacta tcctttatat tgaaaagctc tttaaatttt
     5821 gaattatatt tatcaatttt tccatccaca tcaatagaca caacagcttt gtccatattg
     5881 tttaatagtg tctctaattt tttcttttct aattcaagct cataagtttt aatttgtgct
     5941 tttaatttat tagatattaa gtctgccatt ttgcctaaaa agtttataag accactttta
     6001 ttattttcta taatttgtgc ttgttctttt gtaaaggcta taaggcctat aacgccatga
     6061 gaatcgccat cacatataat aggacaacac acctctgcaa actctacaca agccccactt
     6121 ttatagcatt ccctacatac atcactactc tttgggtcat caattagtat tgtgatctgt
     6181 tctgtaagtg attttttaaa agccgtatat ttatctactt tctcacctat tttgtctata
     6241 tatactcctg tacctgcaat cctaaataac tccttattta ctatagttac atctatattg
     6301 agtactgata ctatagcttc agctatattt tgaatatcag attttatata ttctaaacct
     6361 atcataacca tcattcctta tttttcttta acataagtat ttgtcttatt tctttaagaa
     6421 tcaagtatat cttatttaaa gtaaattgga ttgacatcag gattacaaag agcataacaa
     6481 ttattattaa ctcataattt atttccatct aaacatctcc ttataacaaa agactaggct
     6541 taacctagtc ttttatatta tatccatatt tatattttaa ctcatttatc ttctttttcc
     6601 cacataaata tattttaatt tatttgactc tttaatcata ccatcaaagt ttaatcttga
     6661 aaatatatta aatacttcat gtggatagtg aatctttgca acagaaccac aaaatttatg
     6721 tttaagataa tttaacttaa cttttaaatt ctttaaattt aaatcctcta atataacctc
     6781 tccattgccc tttaacactc tttgtatttc agatgaagct ttttcacaat ctgtaataac
     6841 attaaattgg tcaactatta ttatcttgtt gaactgatta tcatcaaatg gtagaaactc
     6901 taacttacct ttaactacat tgcaattttc attatacact agagaataac agacatcttc
     6961 ataattacta ttctccagta tagttacaga agttccaaga cctctcaatc tcttgcctag
     7021 gtttcctata tgaccaacaa gcaatatttt atcacctaca ttatactcaa ttgtatccaa
     7081 aaaacattct aattcatcag atgtatattt agctttaact ggctgtttat ttatctgaca
     7141 taatatgctt ttcacaatgc ttcacctctt catttcctca attataaatt atgtacagat
     7201 tacattataa taaatggcga gcaactattc aagagatatt tcattagtat ttttttaaac
     7261 aatctttata cattttaata tgatattata ccaaatttat taggatttta ttcataaaaa
     7321 tcagtctatt ttttatgttt attcattgtt attttcatcc aattttttta ttttcgatat
     7381 ggatacttca tatgccattt ttttgaccac attattttca tctatttttt tctcatattc
     7441 tctactttga accctacccc aaagctgtat tctatcacca acctttagat tctttgcaaa
     7501 tttagcattt ctaccccaaa ctattgatgg tatataatca gatttattat aaggtctgtt
     7561 tatagcaact aataaatcgg ttatttccct tcctaaaggt gtcttcctat acacaggttc
     7621 cttacataca tatccatcta aaaatatact attagggtct ttattttcct cgtctatgct
     7681 ttttatttct ctgacaaaca ctgtaagcac taatctattt ttattattta atgttttgtt
     7741 atatgatctt aattggccta cgacattgac taaattttgt ttatcaaggt caatatcttg
     7801 gagaagtctt tctgatacag tcattggaag tatatcaaaa gaatcgctta gtctgtttat
     7861 ttttattttc atattataaa atttttctcc aaatatctca tggctaaatt ctagcttatt
     7921 gtctaactcc cctcttaagt ttactacgtt attctcatct ttaacagcta tcatatttta
     7981 tccctccaca attgttaatt cttatatttc tggtatcagc ttatataata aatatattta
     8041 taagggatgt attttattac aaatctttga ttgctgttta aaagttttta tttagttata
     8101 aatttcattt aatatttcac ttttataatt ataaaattat tatctttttg tactacatat
     8161 actaattaaa ctacctattt cagttttgta ttaattataa aaatcctatt atataattaa
     8221 taagtcatat ataacttaaa tttattcact ctatataagc aacttcaacg aattaaattt
     8281 attcaaataa ttcagatgaa tattgagtca ttataaactt atttttctta attataagtt
     8341 atattagact tattacagct tatattttta ttaatctgta ttaatatatg aacatttatt
     8401 gaacttattg attataaaat ttaaatattt agctttaaat tatgctttat tctttgaaaa
     8461 tacaacgttt tttacattaa atctactaaa tcactatata tattacataa ttggcatgtt
     8521 aattgcttta tattaattta gcaaataaaa aac
//
LOCUS       DAEPLF010000148         8529 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEPLF010000148 DAEPLF010000000
VERSION     DAEPLF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.148"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..830)
                     /locus_tag="KR314_002704"
     CDS             complement(<1..830)
                     /locus_tag="KR314_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBH0329074.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     "
     gene            complement(902..2032)
                     /locus_tag="KR314_002705"
     CDS             complement(902..2032)
                     /locus_tag="KR314_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0329075.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     regulatory      complement(2260..2441)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2494..3387)
                     /locus_tag="KR314_002706"
     CDS             complement(2494..3387)
                     /locus_tag="KR314_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBH0329076.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNKLNIC"
     gene            complement(3467..4369)
                     /locus_tag="KR314_002707"
     CDS             complement(3467..4369)
                     /locus_tag="KR314_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBH0329077.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESIFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            complement(4419..5579)
                     /locus_tag="KR314_002708"
     CDS             complement(4419..5579)
                     /locus_tag="KR314_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH0329078.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            complement(5735..6040)
                     /locus_tag="KR314_002709"
     CDS             complement(5735..6040)
                     /locus_tag="KR314_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH0329079.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGNEISFKLKDKTVKTITALDNI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            complement(6138..7112)
                     /locus_tag="KR314_002710"
     CDS             complement(6138..7112)
                     /locus_tag="KR314_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBH0329080.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            7694..8470
                     /locus_tag="KR314_002711"
     CDS             7694..8470
                     /locus_tag="KR314_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBH0329081.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
BASE COUNT         2794 a         1570 c          949 g         3216 t
ORIGIN      
        1 tttgaaattt gtggcactgc aagtccaata aaccctatag gtcctgcaaa tgctgatact
       61 attgctgcta atatactaga tataaacact atcaaaactc taaaaaatct tatatttatg
      121 ccaatacttt ttgcatactc ttctcctaat aaaaaagcat ttaaaggttt tattatgaat
      181 acagtcatca aaagacctaa tataccaact attataagta tttctacctt ttcccatgta
      241 aatccagaaa aacttcccat agaccacata gtaaattctt ttaatttatc actatttgca
      301 aatgattgta acacatttgt tataccacta catacatatc cggtcataat tcctattatt
      361 aaaagagttg ttatattctt taccttattt gcaaataaaa tgactataga cattacaatt
      421 aaagctccta taaaagctgc tacaaatata ctcattgaat ttacactctc aaatcccaat
      481 gtgtatcctc ccaaaatcat gagagctact gataaagtag aacctgaaga tactcctaat
      541 atatacggtt caactattgg gtttttaaaa aatatctgta ataaaatacc tgatactgct
      601 aggcatgctc cacctaccat ggttgcaagt actctaggga ttcttatatt gtatattatt
      661 ttataatgtt ctgtattatc catacctctt aatacaacat ctattacttc tttaaattca
      721 atttttacag agccaaatgc tacacacact aaaaaagtaa gtatcatact tataattaat
      781 actataaata ttgtcgtatt ccttattctc attttattga taaaatccat ttgtatactc
      841 ctcttagcaa agttatatta actaaaatct ataaaaccta tctttttgtg tcctctctgt
      901 actatttatc ataatttata taatgtttga ttttttcacc tgtagtacct gggtggaaca
      961 tctcaactaa atctactatt aattcgtccg tcttatctac tgattgataa taatctggag
     1021 caaacaccca taggtttccg ttttttacaa catccaaatc agccaataca ggtgcactag
     1081 attttataga atctaaattt ggtgcataat ctgaacttga tgagtataca aatatatctg
     1141 catcttttgc aacttcatgc aattgttcaa tacttactcg cccttcattt gaatctattg
     1201 aagcacatgc atttattcca ccagccattt ttatcatatt atccacatat gaatctttag
     1261 gagctatata agcacttcct tcatatacag atgcccatac aactttaggt ttagctttgt
     1321 tttttatttt tttacttact tcttctacct tttgtacagt tttatttagt tcactagtag
     1381 ccatatcttc tttatcataa aatgcagcta ttaaacttgt ccactccatt cttccaaatg
     1441 gattttgctc tttatactca ttgtctacaa cataattaag ccctaattct tttaatttgt
     1501 caataaattt tgtatcagcc tctgataaac cactactcac tatagttaag tcaggattta
     1561 agtctactat tttttcataa tctggtgcta aatttgaacc aacgtaatgt atttttccat
     1621 ctttcattaa atctttaatt tctttaatag tccagtactg ctcttctgtt gttacggctt
     1681 ttatactatc tagttcatct attgttctca gagaacaagc ctgtgttgta gatgctatta
     1741 atacattgtc cacaggcgtt ctaactatat tagcattttt atatttttca ggaattttaa
     1801 tcttctttgg gattaaaatc agttctcgat tatctccatc agttactttt ttaattccat
     1861 cttctaaatc ttcaacacta aatccactag catattttaa ttctaatttc ttaccagtac
     1921 tagcttgttt attctctttg tcacttttac tacaccctat aagacctcct accataaaaa
     1981 ttaccatagc aattaaaaaa aactgcttta aaacatttga tttaaatttc atctctttca
     2041 ctccaatatc ttatttttta tgagtggcta aaaaacttta ataatatatg ctttttaaag
     2101 tatacttatt aaagttatcc taaagtcatc tttaataagt ataacctttt aataaaataa
     2161 aaaacttctc ctaatttaat aaggagaagt tcatatatca actttacaaa aacgaattga
     2221 tacttcattt tattataaat tacaatctcc accctaaatt gaacttatat aatactgggc
     2281 aggtctcctg actcacggct tttcctccat cacccttccc aaaattttta taaatttcag
     2341 tggattttga tttcgtatcc gtatacagta gcgggggctg tagtgaattt tcatcacttt
     2401 ccctattaaa ctaatatagt accctagtat tattttaatt taatttttat taagataatt
     2461 gtaatttatt gttgacctct ttgcaaggtt gatttaacat atatttaatt tattcatcaa
     2521 ttttttaatt tcacatactt gtttttcatt tggttttaca aatggagcag tcatataatc
     2581 acttatttgt aaccctctga tattcatagc ttttttcatt gctggtataa atggattggt
     2641 tattaagtaa aaatccattg ctttattaat atatttttga atttctataa ccttctccat
     2701 atctctatta ttgtaagctt cagcccattt ggagcaaact tctggaatta aatttgataa
     2761 tccccctata caacctgctc ccccagacat tagattatgt ataaagtttt catcataacc
     2821 actataaacc ttaaattctg ggaactctat tttcatagta tttattaaat ctctggtatg
     2881 tcccatcaaa gttactgtat ccttatatcc aactatgttt ttatgttttc ttgcaagtct
     2941 taatgtaagt tctggactta aatcataacc agttctatct ggataattgt ataaatatat
     3001 ttcagcctct gtattcttag ctatttcact ataaaataac tcaatactat catcatcaag
     3061 agagaaataa taagggctta ttatcataac gccatctgct ccactattat gtgcatagtt
     3121 tgaaagttct atagattcat ttatacacat acgacttgta cctataaaca cttttactct
     3181 cttatttaca tgactagtta ctaaatctat aagttctttt tgcatatcca tagacatatt
     3241 aaagaactct ccagtacttc ccattatgac tagtccatct acacctccat ctataagatg
     3301 gtcgtacact tttttattac cttctttatc taaatttcct tcttcatcaa atattgtaac
     3361 aactggtgtc aaaaattctg ctttcataat ttaaactcct aaaatttatt ttaatatgtt
     3421 cattaataca tcagatttag ctttaatatt aattatatta aaacatttac ttaatgtaat
     3481 tttttaaaat tccaatactc tcaatctcac attcaactac atctccacta ctcatatatc
     3541 gaggaggtga aaatcccatc cctactccag aaggtgttcc agtagctata atgtctccag
     3601 cctctaaagt catacctttt gaaagttctg aaactatgtc tgaaacatct gatatgaata
     3661 atcctgtatt agaactttgt ctttcttcac catttaccct actacatact ttaagtttta
     3721 tgggaaaagg aactgcactt ttatgtaaaa tacatggacc taaagaggta aatgtatcta
     3781 atccttttcc tctaaaccat tgaacatgtt ctttttgcaa ctttctagca gaaatatcat
     3841 ttataacagt gtaaccaaag atataatctt ctacctcttc ttttctaata ttaacacctg
     3901 ttttacctat tactactcca agttcaactt catagtctag ttgattattt atctcaaaat
     3961 gaccatctat ctcatcttct ggacctattg cctttgtcac acgttttgta aaataaaccg
     4021 ttttttgtgg ctcttcaaaa ctatcatcaa agtgagtttg agtttcttca agatggtctt
     4081 tatagttcac tcccacacat aagatatcat gtattggttt ttctatagga acatgtacag
     4141 tacatctctc tcttttaaaa acatcataat tatagaatat actttcattt ataatagttt
     4201 tcaacttaat aatatcttca ctagatacat tttgaattaa ttcatttaaa tccttaaaat
     4261 ttctactgag atttaaagag ttaacatcta taataaataa ttcatcttta gaaaaaactc
     4321 cagttctttt acattcattt tctccttcag taaaacttac aaacttcata atctcaccct
     4381 ttattaaaat tctatgtatt tattttctta cttaaagatt atacatagtt gcatactcta
     4441 gctattgcca tttcagtata tccaagggac tctgcttttg attgttttcc atttgctaca
     4501 tctacaacca tatttaacaa atcatctgtt aattcttcca ttgtttgtgg accatagata
     4561 actggactag catcaaaatc catattatct tccatatttg caaaagttat tttatttcca
     4621 gttattttta ttactggtgc tattggattt cctgatggtg ttcctcttcc tgtactaaat
     4681 accactattt gtgctcctcc tgctaccata ccagcaacag atgaagcatc atttccaggg
     4741 gtatccatta taacaagacc tttcttatca atttgtttgg cataatcata tacttcactt
     4801 atttgtctat gacctccttt atgtatacaa ccaagtgatt tttcttctaa acttgttaaa
     4861 ccaccagcta tatttccagg agatggatta ccttctctaa cttcttctcc aaccaatttt
     4921 aatgaatttt catatctatg tactatatgt aatatttttt cttttacttc ttcatttact
     4981 gctctcctag ctagtatatg ctctgcacct ataaattcag tagtttcact taaaattgat
     5041 gttgctccta aatcgacaag tttatcactt aattcaccta ttaatggatt tgctgctaat
     5101 ccacttgttg ggtcagaccc accacattca gttcctagta taagttcaga tattggaaat
     5161 tcttcttttc taagcaaaga agcttcttgt gccatttctc tagcataacg aacagctttt
     5221 tctatagttt ttaaagttcc atgttcttct tgtattacaa gagtttttat tggcttattt
     5281 gttctctctt ttattgcatc aactaccaaa tcattttggc aattttcgca tcccaaagat
     5341 acaactatta caccatatac atttggattt gctgcataac cagccataac atccatagtt
     5401 aattgttggt ctgaatgtac ttgtgaacat ccattctgat tattaaaggt aactgcccct
     5461 tgaacttgtg atgctattat tctagttgta tctgatgcac acacactcgt aggcaatatt
     5521 aatatgtggt ttcttatacc aactcttcca tctggtcttt tatatccata aaatttcata
     5581 ctgatttcct cctaaaatag tctatatact ttttataatt tttatatatt ataattttta
     5641 ttacactcac taatcaaaca gctaaaatcc tatttaaaaa ctatttgaca ctaaaagcaa
     5701 tgaatacaac ttttataact ctataaatat tattttatat attagtcata acttgtttat
     5761 ccaaatcctc tctaacactt ctaacattat gaatatgtac atgttgacca ctttttattt
     5821 cctgatttgc ttctcctata tgttcaccat atttagtaac tttatcacct tgggccatat
     5881 cttttatagc caatttatga taaatagtta tattatctag tgctgtaatc gtttttactg
     5941 ttttgtcttt taatttaaaa ctaatttcat tacctttagc tattgcttca atagctactg
     6001 caacattatc tttttcatca attatcactg catttatcat tattcttgct ctcctttata
     6061 aataatataa ttttattaat atcctaaaat ctaattaaag aatttattat tttaccaaca
     6121 actcactgta atataaatta agctgttatt gtaccatcct cattttgttt tgggaactta
     6181 ggacatccat acttcttagc ccaccaacta gttataagag gtactaatat tgcagtgaca
     6241 actactgcgg ctgccacttg tgttgtagct gttgcaacat atggttgcca tgatgggtca
     6301 actaatgcaa cagcagcagg tacagcaatt gcatttccag cggttgtagc tacagcccat
     6361 cctgcatatc ctggacgttt tcctataaat ttatcacaga atactataaa tatacctcct
     6421 acaaatacag atattaatcc aagtaatact cctgataaac ctccttttag tatacttgca
     6481 agatttatgc tactaccaag tgcaaaccct acaaaaggta cgagtaaatt acaccctggc
     6541 tctaaaaact ctttcatttt tttgtcaaaa tttcctatta tcataccaac aagtattggc
     6601 actattgcag ctaataatga caacaatggc acatttgcaa gacctgaagt cccaagagct
     6661 attaatgtaa aaaatggtcc atcatttaaa gtaagtaaac tcatagttgc acaatctgtt
     6721 tcatctccat atgaagtcat caatgaaaga tatatactac cattactatt tgtaactgca
     6781 cttataacag ccaatgttgt aagaccaagg acaccatcca ttccaaatag ctttcctata
     6841 agtataccta tcaaagcacc tatagcaaat ttagaaccaa gaagtatacc acctctcttt
     6901 aaaacttttg gcatttctct aaattgaagc tgagttccca agcataccag ttgaacccca
     6961 attatagttg cagacccaac acttgaaaat actgctgtcg taaacgaacc tatttgtaca
     7021 atatctgggc aaaaggtatt aatgaaagct gctataaaca ttggcactat catcatacct
     7081 gcaggaattt ttttaataaa atccattatc ataaagttaa tcctcctaaa acatgatatt
     7141 taattactct tattaagttt ttagctttaa ttctaacggc cgtttcggtt cgtatttaag
     7201 aactaagttt ttaattacga atctttgaat taatgatagc attttcctat ttatgagtca
     7261 ataaaaaaaa taaatttttg tcaaattttt ttcaaacttt ctgataatac cttgtatatt
     7321 atcaaatgac ttccttatat gcaaaaatac agcgtattta aaattatata gtattcaaaa
     7381 ctataaagtt tttatacagt gtaaaaaata gataaaatat aaattcataa tattttgata
     7441 tttacatata aaaataacta ttgcatatat ttacttaaat tatataacta aatatcactt
     7501 actttcttta ggtatatttt ggaaattagt tattccttaa tgtacagtct aaaaaaatat
     7561 ctatctgtat ctaaatcaag agcttattta tatataaatt tacatattta ttttctacat
     7621 tgtaaaatat cgtatagtgt gctaaaatca acatattcta atactttaaa ttaataaaaa
     7681 gagggggaaa tttatgactt cagataatca tcgcccaaca gctaggatat tagatatttt
     7741 agaagcatta gcagaatccg aaaatggata tactcttaca gaaatagcag atgttatcaa
     7801 tgctccaaaa agtagcatct ttcctatagt tcatacactt aatgctagaa aatacattac
     7861 tattgataaa aacacatcca aatattctat agggatacgt tcttacacat tagggtcttc
     7921 attttttgaa aaaagaacta tttttaacta tataattgac gaaatgacta atatagttaa
     7981 ttcctgttct gaaacttgtc agcttggtat acttgacaat aatgaagttc tttatattgg
     8041 aaaagtagat tctccagaac caattagact tatttcttat gttggaaaaa aaatacctgc
     8101 taattgtaca ggtcttggaa aagctctttt atgtgatttt gacaaggaaa aactaataag
     8161 cttatatcca gatggtctaa aaggttttac agaaaaatca attacagatt ttgatgtttt
     8221 atacgaccag cttcttgaag ttcaaagaac acaaatagct tcagagtgtg aagaatcaat
     8281 ggaacaccta aaatgtcttg ccgtaccaat aagaaaaaat ggatgtataa gagctgctgt
     8341 tagtgtaaca ctgcctttat ttagagctaa tgacgaaaaa atagaactga taaaaaaatt
     8401 actcttaaat gctcagaaaa atattcaatc aatgatggaa gaacgtaatg aagacattgt
     8461 aataaattag attaatattc tgtatatgga agatatcaaa acataataat taaaaatttt
     8521 gttttaaac
//
LOCUS       DAEPLF010000149         8522 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEPLF010000149 DAEPLF010000000
VERSION     DAEPLF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.149"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            51..410
                     /locus_tag="KR314_002712"
     CDS             51..410
                     /locus_tag="KR314_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329082.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            445..801
                     /locus_tag="KR314_002713"
     CDS             445..801
                     /locus_tag="KR314_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBH0329083.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            838..2130
                     /locus_tag="KR314_002714"
     CDS             838..2130
                     /locus_tag="KR314_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBH0329084.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            2566..3204
                     /locus_tag="KR314_002715"
     CDS             2566..3204
                     /locus_tag="KR314_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329085.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            3211..4053
                     /locus_tag="KR314_002716"
     CDS             3211..4053
                     /locus_tag="KR314_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBH0329086.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            4114..6741
                     /locus_tag="KR314_002717"
     CDS             4114..6741
                     /locus_tag="KR314_002717"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBH0329087.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            6955..7200
                     /locus_tag="KR314_002718"
     CDS             6955..7200
                     /locus_tag="KR314_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0329088.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            7412..7729
                     /locus_tag="KR314_002719"
     CDS             7412..7729
                     /locus_tag="KR314_002719"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0329089.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRSKTQVQN
                     WVKDYKNKGKTAFNEETRGRFKNISLENDNRKFKSVEEELKYLRMENEFLKKLSTLLD
                     KSK"
     gene            7756..>8522
                     /locus_tag="KR314_002720"
     CDS             7756..>8522
                     /locus_tag="KR314_002720"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBH0329090.1"
                     /translation="MSRKYSISSMCKLIGVSRSGYYKWLSYSKKSSDRGIKDRIIKDY
                     IIEIHKKYRGTYGRKRICTYLNKILDSPINHKKVYRLMKELGIKSIIRKKVYRRKFKS
                     YEVYDNILNREFRANQPLEKICMDITYIPIGKKFLYMNVAKDLFNGEIVAYEISTKMD
                     TKLVNKTVNQLINMNLAKDCILHTDQGSQYTSRSYSKRLKDNGIIQSMSRRGNCWDNA
                     PIESFFSHFKSELIYLIDTTDPKEMISLINDYIYFYN"
BASE COUNT         3438 a          881 c         1644 g         2559 t
ORIGIN      
        1 agttgttata cagatttaaa aataatgtga tttttggaag gtgtataaat atgaaattat
       61 tagtaatttt atttgttgct tatattttaa atactatatt tataatgaag cagaataaat
      121 tgtattttaa aactttaaat agagctaaaa aaaagggtga tatagtgtct acaggcaaaa
      181 agaaaagcta tttttctaaa ggaagtatag ccattatatc ttcagattca gatgggttta
      241 taaagtttgg agaaatttta aaagggcgta cagtaatggc taaatttaaa gagattgaag
      301 aaatagaagg attagatata tatagtgcag agcaaaaatt taaagatgaa gagagtattg
      361 tacaagctat aaaatttata aaagaaaaaa taaatgtttc ttttaattaa ctcaatataa
      421 atataaatta tcaggaggaa tgtcatgaaa tatgaagtta agataagtgg gataggcagt
      481 ttagttaaag agttaatgga tgaaagcaat tgtcttatta tatatgatga aaccattaat
      541 gatgacgact taaaggatat atctgtaatt cattcgatag caacattaaa aagtgatgtt
      601 gaaataggag atacacttac tatagggaat agagattatt gtatagtttc agttggtgat
      661 attgctcaga agactcttag agaaattggg cattgtacaa tcaaatttga tggaaaatgt
      721 gaagtaaatt taccaggaga aatacatgtg gaaataggaa atccagatat aactattgga
      781 gatttaataa ctatttctta atttgaaaat aaaattatta taacaggagg aattggtatg
      841 ttagatttaa acagaaaact tgcaaaattg gaagaggaag gtaaaaaaat aaaagtagct
      901 cttataggag ctgggcatat gggaaatgga atggtaagcc aaatggcaaa tatgaaaggt
      961 attgaagctt ctattgttgt agatataaat ttggagttgg ctcataaagc ttttactgat
     1021 gcaggaatag atgaagaaat tatagataat gtaacaaatg aaaaggatgc agaaataaaa
     1081 ttacaagaag gaaaagtatt gacatgtaat gactttcttg tggcttgtaa aactaagtca
     1141 atagatgtag ttatagatgc aactgggggt attgcaattg gtgcagagat agcattaaac
     1201 tctattttaa ataaaaaaca tatagttatg ttaaatgctg aaactgattg tgttgttgga
     1261 ccaatactta aaaaattagc agatgatgct ggagtgatat ttacaggttc tgcaggagat
     1321 gagccaggtg cagtaatgga attatttgat tttgcagatg caatggggtt tgaagttaga
     1381 gtgataggta aaggtaaaaa taataaatta gatttagatt gtaatcctga tacagttaga
     1441 gaagaagcag aaagaaaagg agcttcacct catatgatag cttcttttaa agaaggaact
     1501 aaaacaatgg tagaaatggc tttaatgtgt aatgcaactg gatttgtgcc tgatgtaaga
     1561 ggtggtcatg gtattgaagc tacagttaat gaagtaccta agaaatatgc attaaaaagc
     1621 gaaggtggag tattagataa ttatggtgta gttgattttg taaatggtat agcacctgga
     1681 gttttcgtag tagtagccca taaattaaaa gcagttaatg atgaactaaa atatttaagt
     1741 atgggtgatg gacctaatta catattatat agaccatatc atttatgtag tttagagact
     1801 cctttatcag cagctatggc agtcttagac cataaggcta caatagtacc taaagctgga
     1861 ctagtagcag aagttatgac tatagctaag aaagatttga aaaaaggtga gtttatggat
     1921 ggttatggag catatacatg ctatggtact atagaaaaat acgatgtagc aaaggctatg
     1981 aatgctgtac ctattggttt gataagtaaa aagactaaag ttgtaaaaga tattaaaaaa
     2041 ggtgaagtta taacttacga tatggtagag atagaaaaag atactacttt atatcattta
     2101 agacaattgc aagaaaaaat atttgggtaa gatttgatgg taatataaat agatattagt
     2161 aaaaagggtt gatggttatt tttaaatcaa cccttttaaa tttttatata ttcttgttag
     2221 gatactttct ttaaaagtat attataggtt atttgtagtc gtaaaaatta ttttgaatta
     2281 gttctgcaat tttatattca cattgaaaca atattattaa aaattttaat ataaaatgaa
     2341 ttaaaatgac caattatgta ttataatatt aactgtaata ctaatatagt tctattaatt
     2401 aaagaaatat cccccattaa tatataataa atatctttaa gcgagagaac ttagtaaaaa
     2461 atatatcgta ttcaataaaa aatctgttta cagaatggta tatatagtta tataatatac
     2521 tatataagat gcgaaaagat aaaaagtata gcacaatgaa agaaggtgat tattattcag
     2581 cttaatgaaa gacaactaaa aattatagat atagttaagg aaaatgaacc aataacgagt
     2641 gaaagtattg cctccagctt gaatgtgact cgtgctacac ttagatctga tttagctata
     2701 ttaacaatga caggaatatt agacgcaaga ccaaaagttg gatattttta ttcaggagtt
     2761 agtgaaataa atttaattgg taaaagtgta aaagaaaaaa cagtagagga tataatgagc
     2821 atgcctgtac tagctaagaa ggatgaaagt atatatgatg taatagtcac tatgttttta
     2881 tctgatgtag ggagtatagt tattatagat gagaatgaag agttatgtgg agttgtttcc
     2941 agaaaagact tgctaaaagc aactatagga ggttctgata taaataaaat gccaataggt
     3001 atgataatga caagaactcc aaatgtagtc actttaacta aaggagcaag tgttttatta
     3061 gcttcaagaa aaataataga acatgaagta gattcgattc cgattgttga atataaggga
     3121 gaagacaaaa atcatatgag agttgttggg agaatatcaa aaaccaacat aactaagcta
     3181 ttcttagaaa tagttgacaa ttaaggaggt atgctggtga aaaacctaat tatatatgca
     3241 gtatcagatt cagtagggga gactgcacaa caagttgcaa aggcatgtat gtctcagttt
     3301 tatgtgaatg aaacttatga aataaaaaga tttccataca tgattaataa aggagtacta
     3361 cttgaaactt tggagaatgc aaaagctgaa aacgctttaa ttgtatatac attagttgat
     3421 gaagaattat gcagtattgt tgaaagatat tgcgaaagag aaggtcttag ttgtatagat
     3481 ttaatgacag atatactaag agaaataagc aagagaacag gaagaaaacc taaaagagaa
     3541 gctggtataa ttagaaaatt agatgaatct tactttaaga gagttgaagc tatagaattt
     3601 gcagtgaaat atgatgatgg aaaagaccct agaggtgttc ttcaagcaga cataatttta
     3661 gtgggaatat caagaacatc taaaactcct ttgagtatgt atttagctaa taaaaatata
     3721 aaagtagcta atgttccatt agttccagaa ataccaattc ctaaagaagt gtttgagata
     3781 gatactaaaa agataatagg tcttactaat tcacctgaaa agctgaatga gataagaacc
     3841 caaagattaa aagctttagg attgtccagt aaagcaaact acgctaattt agaaagaata
     3901 cttcaggagt tagattattc agaagagata atgaaaagaa ttggatgccc agtaataaac
     3961 gtttcaaata aggcaataga agaaacagca ggtataattt tagacataat gaaagaaaat
     4021 ggtctaaaga tatacaaaga aatagaaatt taataatgtc tattaataaa aatgatataa
     4081 attaaaataa aaagattaat gggggattta acaatggaaa ctaagtatgt ttatagtttt
     4141 ggtgaaggaa gcaaagacat gaaatcttta ctaggaggta aaggtgccaa tttagcagaa
     4201 atgactaaga taggcttacc tgtccctcct ggattcacta taacaacaga agcatgtaat
     4261 gattattatg ttaacaatga aagtataaga gcagagataa taaaagaaat agaagagcat
     4321 ttagctactt tagaaaaaga cttaaataaa acattaggat gcaataagaa tccattatta
     4381 gtatcagttc gttctggtgc agtattctct atgccaggaa tgatggatac aattcttaac
     4441 ctaggtctta atgacaatag tgttgttggc ttagcagagg ctacacaaaa tgaaagattt
     4501 gcatatgata gttacagaag attcatacaa atgttttctg atgttgctat ggaagttcca
     4561 aaatataaat ttgaaaatgt attagacaga gtaaaagaag caaagggata cacagttgat
     4621 acagaactta caactgatga tttaaaagaa atagtaaaag aatttaaagc aatttataaa
     4681 aaagaaataa aaaatgattt cccacaagac ccaaaagagc aattaatgct tgcaatagag
     4741 gctgtattta gatcatggaa taatcctcgt gcaatcgtat accgtaaatt gaatgatata
     4801 gctcataact taggaacagc agtaaatatt caatcaatgg tatttggtaa tatgggagaa
     4861 actagtggta caggtgttgc atttactaga aatccagcta caggagaaaa taaattattt
     4921 ggtgaatttt taatgaatgc acaaggtgaa gacgttgttg caggtataag aactcctcaa
     4981 aatatatcaa ctcttgcaga tgtaatgcca gctgtattcg atgaatttgt aaaaattact
     5041 catatacttg aaggtcatta taaagatatg caagatatag agtttactat agaaaatgaa
     5101 agattatata tactacaaac tagaaatggt aagagaacag cagcagcagc aataaatgta
     5161 gcggttgatt tagttgaagc tggaataatt gatgaaaaag aagctatcat gagaattgag
     5221 cctaatcaat tagaccaatt attacatcca aaatttgaag ataaggcatt aaaagaagcc
     5281 aaggttatag caaaaggttt accagcatca ccaggtgcag caagtggtaa agtttatttc
     5341 aatgctgatg atgtagtaaa agcaaatgaa aaaggagaaa aagtagtttt agttagatta
     5401 gagacatctc cagaagatat agaaggaatg gttaaagcag aaggaatcct tacagctaga
     5461 ggtggaatga catcacatgc agcagttgtt gctagaggta tgggtaaatg ttgtgttgct
     5521 ggatgtggag aaataaaagt tgatgagttt aataaagaaa taagagcact tgatgatgta
     5581 gttataaaag aaggtgaata catttctata gatggttcta caggaaatgt gtacctagga
     5641 gatgttaaga aaacagaagt atctttaact ggaaactttg aaaaattaat gaactgggta
     5701 gacaaacaca aatgtatgat ggttagaact aatgcagata atcctagaga tgctcgtgca
     5761 gctatagagt ttggtgcaga aggtataggt ctatgtagaa ctgagcatat gttctttgat
     5821 gaagctagat tgccagcagt tagagaaatg atattatcta atacagtaga acaaagagaa
     5881 aaagctcttg aaaaaatatt accaatgcaa agagaagatt ttgtagagtt atttaaagtt
     5941 atggatggaa aaccagttaa tataagatta ctagacccac cactacatga atttttgcct
     6001 catgatgatg agactataga agaactttca aaatctatgg gaataaaagt aagtgatatt
     6061 aagaagagaa tagttgattt agatgagttt aacccaatgc ttggacacag aggttgtaga
     6121 cttgcaataa cttatcctga aatatgtgta atgcaatcta aagctataat acaaggtgct
     6181 atagaagcaa tcaaagcagg tgttaaagtt tctccagaga taatggttcc attagttgga
     6241 gaagtaaatg agttaaaaat aataagaaaa atgatagtag aaactgttga cgctataata
     6301 aaagaagaag gtatagaagt tccttatact gtaggtacta tgatagaaat acctagagct
     6361 tgtttaactg ctgatgaaat agcacaagaa gcagacttct tcagttttgg tactaatgac
     6421 ttaactcaaa tggcatttgg atattcaaga gatgatgctg gtaaattctt aggtcaatat
     6481 gtagatgaag aaatattaga aaaagaccca ttccaagtac tagaccaaaa tggtgttggt
     6541 aagttagtta agatgggagc taaattaggt agagaagtta aaccagaatt aaaactagga
     6601 atatgtggag agcatggagg agaaccatct tcagtagagt tctgttatag tgtaggactt
     6661 aactatgtat cttgttcacc atttagagtg ccaattgcta gacttgcagc agctcaagca
     6721 tctataaaaa atcctagata ataatagact gcaaaatatg taactaaata aaaataaaat
     6781 cccctattgt tcaatgtatc aaattaagta tttgtaaatt gaatataggg gatttttatt
     6841 ataaaaattt aacttataca gttgtaagtg aaacgtattt aattatattg catatattat
     6901 tgtagagaga aatttcaact atcaattaaa ttgagtttac tggaggggat aaagttgaca
     6961 aagtgtccta aatgtggaaa ggaagtatca tcacgtccag gtagtatatg cccacgatgt
     7021 ggatttaaag tagcagatga gagtctaaaa ataagatgtc cagaacctag ttgtagggct
     7081 ttggtatcta ggaaactgga ttattgtcct aaatgtggat gcaaacttag aggatataat
     7141 atttctgaat ttgttaacat ggtaagatgt aaaatagatg agacatttga taaaaaataa
     7201 ttaaatttag agttaagctt ttgagggtgt ctcaaaatga actttttagt tcagaaggca
     7261 ctctttttgt atggaatcaa atatattttt ataatttaaa caataaaaaa gaggcttatc
     7321 tctattttag tataatcccc ttatagtaga catttaaata aaaggctatt ataataagtc
     7381 taaaaagcgt atactctaag ggggtttttc tatgtctaaa aaacataaaa aatatagcaa
     7441 agaacttaaa ctaaaagctg taaatttata tataaaagaa ggatactcat catacaaaat
     7501 tgctgagatg ctgaatataa gaagcaaaac acaagttcaa aactgggtaa aagattataa
     7561 aaataaaggt aaaactgcat ttaatgagga aactagaggc agatttaaaa atattagtct
     7621 agaaaatgat aatagaaaat ttaaatctgt tgaagaagaa ttgaagtact tacgcatgga
     7681 gaatgaattt ttaaaaaagt taagtaccct attggacaag tcaaagtaag cgataaattt
     7741 aaaacaatta aacttatgtc taggaagtat agtatttcta gtatgtgtaa gttgataggt
     7801 gtgtcaagaa gcggttacta taaatggcta agttattcta aaaaatcttc cgataggggt
     7861 atcaaagata gaattattaa ggattatata attgaaatac ataaaaaata tagaggaact
     7921 tacggtagaa aaagaatttg tacttattta aataaaatac ttgatagccc tattaatcat
     7981 aagaaagtat ataggttgat gaaagaacta ggaattaaat ctattattag aaagaaagta
     8041 tatagacgta agtttaaatc atatgaggta tatgataata tattaaatcg tgaatttaga
     8101 gctaatcaac cattagaaaa aatttgtatg gatataacat acatacctat cggtaaaaaa
     8161 ttcttataca tgaatgtagc caaagattta tttaatggtg aaatagtggc atatgagata
     8221 agtacaaaaa tggatactaa attagttaat aaaacagtaa atcaactaat aaatatgaat
     8281 ttagctaaag attgtatttt acatacagat caaggttcac aatatacaag tagatcttac
     8341 tctaaaaggc ttaaagataa tggtattata caatctatgt cccgaagggg caactgctgg
     8401 gataatgctc ctatagaaag ttttttcagt cattttaaat ctgaattaat atatttaatt
     8461 gatactacag atccaaagga aatgattagt ctaataaatg attatattta tttttataat
     8521 aa
//
LOCUS       DAEPLF010000150         8420 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEPLF010000150 DAEPLF010000000
VERSION     DAEPLF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.150"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            362..826
                     /locus_tag="KR314_002721"
     CDS             362..826
                     /locus_tag="KR314_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0329091.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLS
                     KEDKRKNFISFINDNKELIGFINLLDEGSYVFFGIGIKPDYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYKSQGFKIVETQVQKTHLGDGEFFVMRYQ"
     gene            complement(1093..1737)
                     /locus_tag="KR314_002722"
     CDS             complement(1093..1737)
                     /locus_tag="KR314_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329092.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKSLSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPIKRKIR"
     gene            complement(1667..2956)
                     /locus_tag="KR314_002723"
     CDS             complement(1667..2956)
                     /locus_tag="KR314_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329093.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIDEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINLIWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFIMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(2953..3825)
                     /locus_tag="KR314_002724"
     CDS             complement(2953..3825)
                     /locus_tag="KR314_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329094.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILDSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            complement(4355..4846)
                     /locus_tag="KR314_002725"
     CDS             complement(4355..4846)
                     /locus_tag="KR314_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBH0329095.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDENIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
     gene            complement(5940..8075)
                     /gene="rnr"
                     /locus_tag="KR314_002726"
     CDS             complement(5940..8075)
                     /gene="rnr"
                     /locus_tag="KR314_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBH0329096.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKIKKTFEHVV
                     EDFKNAEILAKILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         2832 a         1324 c          861 g         3403 t
ORIGIN      
        1 atttgcctat aatttaccta ctacttggag tattggagca aatacaatat attaataata
       61 agttcctaaa accaataaat taactaatac acataaagat attgatgttg attatacaac
      121 tataagaaac agatttaaca gaatttaatc aagctaagaa attacatata tcgtgatgtt
      181 tttgaattag aaggtaattc aaaattagtt tatagtcttc tgtgaatcca ataaaaagta
      241 cagggtacaa gaattgttag taaaattttt aaagatttta tggatagata taaatagttt
      301 tacttatttt gtaaaattta aaataccatt aattacctta aatattttaa taggaggaca
      361 tatgaattta aataaagtta aatttactga agaacatgct attgaagtta ctaattggaa
      421 atatgaaggt aaatattcaa tatacaacct acctccatgg gaggaaatta aaaagaagaa
      481 tttttcacta tcaaaagaag ataaaagaaa aaattttata agttttataa atgacaataa
      541 agaattgata ggttttataa acttattgga tgaaggaagt tatgtattct ttggtattgg
      601 tataaaacca gattattgtg gaatgggaat tggcaaagaa ataattagcc taggattaca
      661 agaatgtaga aataaatata gtactaaacc aattgtatta aatgtaagaa cctggaacat
      721 gagagctgta aaatgttata agtctcaagg atttaaaata gttgagactc aggtacagaa
      781 aacgcattta ggggatggag aattttttgt tatgaggtat caatagttta taacactagt
      841 catattgcta atatgacaat taaaatgaaa aaatacatgt gtgtatgtca tagcaataag
      901 tgtctttgaa atataaattt gagagccttt gataaaattc ggtatacata tatattttat
      961 actacagata tataaatcta tctacataca taaagagatt gtaagtaggt ttgtgtatat
     1021 aaaaataaat ttatatttca taaatgtact ttttatttgt attaccttta ttttatctaa
     1081 gctctcattt tattacctaa tcttcctttt tattggtttt attaagagag atgcaattat
     1141 caacacaaca cttccaagta tcagccaata tttctcatca aaactacctt gactcataga
     1201 cattataaaa aattttccaa catacttgcc tttagtcatc atatttaaaa ttaaatatcc
     1261 tatagatatc atgtaaccta tgattgtatt attaaataaa tttgagaaaa acattcctaa
     1321 acttcctata aacatagctg atgcgaaact tccaataata tactcaacta ctggaaaatt
     1381 acatccaaga tatattaaaa catttacaaa acctatgatt aataccacta tacctataat
     1441 tgacatcagt atcctaatta attgtacaat aacttgagat aatgattttg aagatactat
     1501 ctcatcaatt tcttttgatt gctctagcga aaatacaggt accaacatta ctatccctat
     1561 tattgcaaca taattttcta aacatatagc tgttgaaatt ctgtctaaat tagctactcc
     1621 ttgtattaga ggaatcatac atagcaatat aattgaacat attaaattag ttaaagtgct
     1681 taatttgaag ttttctttta ttagaactaa acagttttga aaactttgcc caaacatcaa
     1741 gattccccct tcttttttgc tcatataaga atatagctaa caaaacaagc accaatgctc
     1801 ccaagcttat cacaattcta ttaatcataa tttgattgaa gttttgtata ataatatccc
     1861 catttaatac actattatgt cttatcatta gccccattgt tgaataatca cctttgagtc
     1921 ctgtagaatt tatactgtaa agccaccaaa ttaattgcac aataattgct actggtgtat
     1981 ctgttaatat agtaatactc attcctacag ctgtaactat cataagagtt ggtagtatcc
     2041 accataatgt atattttata aatgctaagt tatcaataga tataccttta tctttagcaa
     2101 agaaaatcaa aggaagtaaa tctttcatac ctaaaattaa tacaggcaat atcattataa
     2161 agacaagtgc aaaatatctt gataaaacta ttttgcatga agatacttgt tttgaataaa
     2221 taatctctct cattcttgaa cgcttatctt taagtgacat aaaaactgcc acaaatactg
     2281 gaaatatact taaaactata cctatataat cgcaaaaaat tcttgcatac ccattactaa
     2341 ctttatcttt ttgtattgta tagttgtaat tcttcatagc ttcatcatag gtaactggaa
     2401 ctactccaaa tctttgtaac ttgtcaaaag catatgcaga ccctcctcct aaaatgtcgt
     2461 ctactctttg cattatttct tcaaatcttt caagagatac tttagaataa tctattttta
     2521 tactttcatc agttttccaa attaaattta tttcgctaat acttttacca gttatttctt
     2581 ctatatattt ttctatggtc ttcgttttat tttggtctaa ttttatatac ttaactaccc
     2641 ctgttggata cgttgtatat gtgttttttt gatattctct aattaggttt atagttgcat
     2701 ttggaatgac cttttctgtt ttcatagatt ctttttgacc atatggatat tgattgtgta
     2761 cagagtaata ttcgtcaata ttctctactt tttgtggagc aactaatgga tttccatcat
     2821 taggaatatt tttacttaca tcatttgaaa ctgaacttcc aagttgagaa atatagaaaa
     2881 aaactaaaac tacaataaaa atataatata ccatactctt taatatattt ttacattctt
     2941 ttataaatag attcatatat tatctcctcc cattataagc atatagccat cttcaatagt
     3001 tggattacaa tttactgctg tctcaaaagg tttatcatct gatataatcc taagtatagc
     3061 ctttccacca tgagtcaaca ttcctataac tataaatcta ctttttatat tttcaatgtc
     3121 ttttttatct acttcaatac tatatacttt cccttcagct agttttgcta atgcttctgt
     3181 tgagccattg tatatgactt ttccactatc caatatagct acgttttcac atgtagcttc
     3241 tatatctcca acaatatgtg tagataaaat aacgattcta tcatttgcaa tttcgcatag
     3301 taaatttcta aatcttatac gttcttctgg gtcaagacct gctgtcggct catccactat
     3361 taaaactctg ggattatgaa ttaatgcttg tgctatccca agccttctat tcatacctcc
     3421 agataaagat tttactttca cattttgata attagtaagg tttactttgc ttaataactt
     3481 tgctataatt tttcttcttt ttcttagtgg tatatccgat aaaactgcta aataatccat
     3541 tgctttatat actgtcatgt taggatacat tgaaaaatct tgtggcaaat aaccaaccat
     3601 atttctaatt tccttagtat tctcaatagg aactccacac atatgtattg aaccttctgt
     3661 ttttgataat aaagtggcta aaattctcat aaatgtggtt tttcctgctc catttcttcc
     3721 taaaagacca aacataccac tgtcgatttc aagatttata ttatcaagag cttgtttttt
     3781 cccaaatctt ttactaagat tggatactgc tattttcatt tccatatata aatacacccc
     3841 cagaaataat tttatagaat tttattttta taaattttta ctgtattttc ataataaatt
     3901 ctaaaactta agatattatt tataaatttc taaatttttt ctaaagattt taatttttct
     3961 gtttttaatt aaattaaagt atctttgttt ctgaaatttt ttaagtttat gaagctataa
     4021 tttaatatat atttatttta tttgtgagtc tatttttata tataatgaaa aatgtgataa
     4081 gtatccaaaa gaattactgg atataataag atagggtatt tagcctttta agataaataa
     4141 aaatatctgc aactatatat tgcaaaaatt taaaactaat ttatatatgc taatataaat
     4201 atttataaag acataagaca aatttaccta cagatttaaa tttaacttat atattttaaa
     4261 aggctaatat aatatgtatt ttcttctata caatcaaaag gcttcatatc taagatatgt
     4321 tatgacacta atattcacaa ttttttagta attatcacat tataaaaaat attacagcta
     4381 gtccatcatc agatgcttct tgtataaact ttttaagctc aattagattt gcccatacat
     4441 attcaaaaaa atcatcttca ctctcatcat tcataacagg ataaatatta ttttctaaca
     4501 tctcattaat attaaatctt tttctaaatg cttgttcttc caaactgttc attgcttttg
     4561 atagtttttt tacatcattt gctgtaatca acatgacagg aaattctcca tcaatttcca
     4621 taagtatgtt tccacttagt actaatttgc taaatatatt ctcatcatct ccaccaaaag
     4681 gacaacctgt caaagtaaag tgaatggcat gccatgcttt atcaatattg atagtactat
     4741 tttcgttggc atcttcaaaa acaaaatctt gtaaattttc ttctgcttgc tgtaatttaa
     4801 aaacattctc ttcacttatt ttagtataac atcctatcat ccccataatt acattcctcc
     4861 taagtagcgt ttttttaatt gtaacttata attaaactta gttacaataa aatatattga
     4921 gaaaagtatt atttcatctt aaatttaaat acctatatta tcaactaaaa ttctgtgttt
     4981 atagcgtaag tattattatt ttttcctaaa aattaaatga atcagaagtt ctataggcaa
     5041 tattgactat aaataagaac tattactttt aaaaaaaatt taagaaatta tactgtagaa
     5101 tcatgctcta cttattagct tcaaataaaa aataaatcat gttctcaaat taaataataa
     5161 ataacaataa atttaaattt taaactataa gagtttctga agtgttaata agcataccaa
     5221 taattatatg tgtaagaatt tgatttaata gatatgaata atttttacat taattataaa
     5281 tatattaacc tattattata aattctataa aaagaaaagt tatcttaaat aaaaaataag
     5341 tgtatcttaa attttaaatg atacacttat ttttatattg aaattatatt ttttaatatc
     5401 tattcttaat taggtatttt taaattatct gtttacatta aatatctctt aattacttat
     5461 ctttcagcta gcttcttcta ttaaatgttt ctcaattact tatttttcaa ttagccactt
     5521 ctattaaaca tttcttaatt acttattttt caattagcca cttctattaa acatttctta
     5581 attacttatt tttcaattag ccacttctat taagcatttc ttaattactt atttttcaat
     5641 tagtcacttc cattaaacat ttcttaatta tttatttttc agttggcttt ttctattaaa
     5701 tgtttcttaa ttacttatct ttcagttagc tctttctatt aaatatttct caattactta
     5761 tttttcaatt agctactttc attaaatatt tcttaattat ttatctttaa gttaactatt
     5821 tccattagat atttcttaat tacctatttt tatcatttat tttatagagt attgtgcact
     5881 atatatttat acatatcata aatactacaa cttgttatct cttattttag ctgttttatc
     5941 tactcctcta ttttttcaac tatctcaaag tcaatttctt taaaatctac attaacactt
     6001 tcaaccttta tcttaacaag gtctcctatc ttatatgact tcttagttct ttctcctaaa
     6061 acactataag aactctcatc ataaatatag taatcatcat tcatatttgc aagtcttatc
     6121 aatccttcta tagtattatc taactcaaca aatattccaa atgaagttac gctagataca
     6181 acaccctcaa attcttgacc aactttatcc ttcatataaa gagccttata gaaatcatgt
     6241 acatctcttt cagctaactc tgcctttctt tctttctcgg atgattgaac agatgcataa
     6301 tccactatag tagtaagctg ttcttgtctt tttccactta ttttattatt taaagattct
     6361 tttattattc tatgaatttg aaggtcagga tatcttctta ttggtgaagt aaagtgacag
     6421 tagaatttag ctgctaatcc aaaatgtcca ctacattctg gtgagtactt agcttgtttt
     6481 aaagaacgaa gcattatagt actgatagct ccttcttctt ttttgccctt tattttctct
     6541 attatacctt gtaatgcttt tggatgtact tcctctttat caccttttat tatatatcca
     6601 aatgtagata taaacttact caattcttcc attttttcca aagatggtgt ctcatgtatt
     6661 ctatacacaa acggtattcc catccagtaa aaatgttcgg caattgtctc attacttata
     6721 agcataaatt cttctattat cttattagat attcttcttt catatggctt tatatctaca
     6781 acttcgcctt ttccatttag aattatcttt gcctctggga aattgaagtc tatagctcct
     6841 cttttttctc ttcttgacat aagaatctta gctaaaattt cagcattttt aaaatcttca
     6901 acaacatgtt caaaagtctt tttaattttt tcatcatcct tttctaatat atcagatact
     6961 tctgtataag tcattctagc ttttgaattt attacagatt cacatatttc atgtttaacc
     7021 acatttcctt tatggtctat ttccataaat atagaaagag ttaacttatc ttcaaatgga
     7081 tttaagctac atactccatt tgaaagagtt tttggcaaca ttggaattac tttatctact
     7141 aaataaactg atgtagctct ttttaaggct tccttatcaa gtttattttt ctctcttaca
     7201 taatgtgtaa catcagctat gtgaactcct agtttgaagt ttccattagg taatacttct
     7261 attgatatgg catcatctaa gtcttttgcg tcatctccat ctatagtaaa tatgtttaaa
     7321 tctcttaaat ctcttcttct ttttatctct tcttgaggaa tctcaacagc tacagcttct
     7381 gcttcttgca atactttctt tggaaattct tctggaagac catgttctct tattatagaa
     7441 tctatttcta cgcctctttc acctttttga cctagtatct ctataatttt gccttctggt
     7501 tttctatcct cttgtggcca tactgtaatt tcacatacta ccttatcatt atctactgct
     7561 ccactaaaat gttttttagg tatatatata tctttagtaa atttcttgtt gtctggtgtt
     7621 acaaatccaa aagttttact tgattggaaa gtacctacta ttctagttat ttctctcttt
     7681 attactttta ctattcttcc ttctgctctc ttttcttcag ttgcaggtac tactatttct
     7741 gccataactc tatcgttatg catagcacca tttatattgt cacttgagat aaataaatcc
     7801 tgtgtatatt caacatcaga ttctacaaat ccaaaaccct ttttgtgtga tacaaatttt
     7861 ccaacaaagt atcccatttg attgggagat actatttttc cttttttagt aaagcagata
     7921 taaccatctt cttctaattc atttaaaaaa ttataaaaca ttggcatttc tgatgaatgt
     7981 atatcaaata tttctgctaa ttcttctttt tttagaggat tatatgcact ttcatttatt
     8041 aatcctagca aactttcttt aagtcctggt atcatttttc cctcctgttt tacttttcat
     8101 tatattatta tttatattgt cttttatatt tcttcagata aaacattttt tacttatcac
     8161 ataattttct atattttctt attattatta caagttaaaa ttacttattt atatttttga
     8221 tttaatgtaa gctttgtcat actaacttca aacaaattat gatgttttat aaattatttg
     8281 taaatatcat tataattact taagttattc tttatcataa ttgcttaaat tactctttat
     8341 ctcataatta tacaccatca aataggattg gtaaaatatt catctcatat aagtattact
     8401 atataataat ttattctatt
//
LOCUS       DAEPLF010000151         8273 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEPLF010000151 DAEPLF010000000
VERSION     DAEPLF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.151"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            558..1043
                     /locus_tag="KR314_002727"
     CDS             558..1043
                     /locus_tag="KR314_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329097.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFDLDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(1243..1782)
                     /locus_tag="KR314_002728"
     CDS             complement(1243..1782)
                     /locus_tag="KR314_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0329098.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEASCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVAAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(1775..2188)
                     /locus_tag="KR314_002729"
     CDS             complement(1775..2188)
                     /locus_tag="KR314_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0329099.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(2224..3345)
                     /locus_tag="KR314_002730"
     CDS             complement(2224..3345)
                     /locus_tag="KR314_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBH0329100.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            3944..5596
                     /locus_tag="KR314_002731"
     CDS             3944..5596
                     /locus_tag="KR314_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBH0329101.1"
                     /translation="MNNKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYKMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSDILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKIEYEITINAITGKILEFEIDD"
     gene            5893..6642
                     /locus_tag="KR314_002732"
     CDS             5893..6642
                     /locus_tag="KR314_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329102.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFDIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYAASFANRILIFKDG
                     HIIKELNKQPNNRHTFYELIIQEIAKLDTKR"
     gene            6655..>8273
                     /locus_tag="KR314_002733"
     CDS             6655..>8273
                     /locus_tag="KR314_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329103.1"
                     /translation="MNIFYMAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFITGLVLGALSHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAISNGKFLYKTSLVKLIRHEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQKSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLINGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHR"
BASE COUNT         3059 a         1180 c         1115 g         2919 t
ORIGIN      
        1 atttaccaac atgagaattc ttgttttgag aatttacaga ttaaagttat ttttcaatct
       61 taaattatca agttattaca tatgaaagaa ctatagttga caatcccact attataaata
      121 ataaaacctt tacccatacc ttatttaccc tagttatcat agagcctatt gaaaatataa
      181 cagctaaata taataataac caaaaaatta tcaatatcta ccactgttgc tgaaaatcca
      241 aaaaactacc ttttgatagt attacttaac aatctaatta atctcataat aaatttacaa
      301 cttataaata aattgttata ttggccgtat ctcaacaact ttctcctttc ctagttttaa
      361 atcttttata gaattgtacc acgtctaagt aaattttcat actattaagt cttatttcta
      421 ataaatcatt agctctcatt ctatgcccaa taatgtaaga ttgtagtaat tttattgatt
      481 ttaatagttt tatttaatat acttatatta attattttca tgtgaaatta ttacttatat
      541 agggggattt ttttaatatg agcaaaagtg aattaacggc agaaacaaca gaagaaatgt
      601 tagaagtact aagtggtaaa gattatgata ttgcatgtca tttacatgaa cttggtaaat
      661 cattagattg taaaattgaa ccaaaaacag gtgctcgttc ttacaaaata gtatattcaa
      721 ctaagaaacc aaaacgtagc ttatttacta ttgaatgtaa tgaaaaaaaa tggagagtta
      781 aagcaaatct ttttcatcta aatacatata aagatgctgt ggaggaatgc tctaaaacta
      841 ttaaagatag tataactaaa actcgtactt gtaagaaatg taattcaaag tgtattggag
      901 gttcttcgtt tgatttagat ggaaagtctt atctgacttg cataggaagt ggtcattatt
      961 ttgcaaatat ggaagaaatg gattggaaaa acctagaaaa attaattact aaagaaaata
     1021 atattatgca ggaatctgta tagtttttta agggtaatta ttaaaatata tgagtaacaa
     1081 cagatactta aataggcata aaaaaataaa gggtataata aggatataga ataaaaataa
     1141 atgatatagc acaaagaggc tgtctcaaaa tatagataag cctcttttta ttgttgaaat
     1201 gataagaata tattcgattt tatacaattt tatacaaaca tactaagcca acttacccat
     1261 ttcatatgcc tgtttcattg ccttacttcc cttaatatca cctacattcc aagcacctac
     1321 accatatact ataccttttt ctttagaacc atttagacat gatgtaaatc ctctaaatcc
     1381 ttctattgtt ctttccatat cagccacatt ttcatctgct gctgcaacga taaaatagaa
     1441 atctttatca caaatttcag tatatctaga gcaagttcta tcaatcatag ttttcatttg
     1501 cgcattcata gtataaaaat aaacgggagt cgctaaaaca atcacatctg ccccaatcat
     1561 tttttcaaga atttctccca tgtcatctgt ctgtgaacaa cttgcttctt tttcataaca
     1621 agcaccacat ccagtacaat agttaatatc tttatctttt aaaaatatct tctccacttg
     1681 atttttagat tctaataccc cttgtataaa ctggtcacac aataagtcag aatttccacc
     1741 ttttctagga ctagctgata ctattaatac atttttactc ataagatact gcctccattt
     1801 tcttatttct actgctcttt aatacttttt ctttctcaat aataccttct tcatatctat
     1861 caatcttaaa atttaatcgt tccaaagttt tttgcatttc ttctattctt tttgctagtt
     1921 ggtttctctg ctctattaaa agttcttttc tagctttgat agtactgctc ccttgttgaa
     1981 acatagctac atactcaatt aatgtctcaa tagaaagacc tgcacctctc atacacttta
     2041 tgaaattaac ccactcacaa tcttcttctg taaaagacct aattccactt ttatttctat
     2101 ttacaggtgg tattaaacca attcgttcat aatatcgtaa agtatccgct gaaagctcgt
     2161 attttttact aacttctgaa atcgtcatac atatcttctc ctcttgatat tgtatagatt
     2221 gaattatttc tcaaaggttt ctgctacatt ctttaaagca tctataattt taatgtgttg
     2281 aggacagctt tcttcacatt gtttacattc aatacaatct gaggcctttc cataagtttt
     2341 agtataatta ttatagtaaa ccatctgtgt tgaaaatcca gtattaagag cttgtttttc
     2401 tgcattgtac agcgcaaaat agtttggaat agcgatattt tttggacagc cttctacaca
     2461 atactgacag gcagtacaag gaactaaaat tgattcgttg ataatctcta ctgcttcatc
     2521 tataatatta tattcctctt gtacgaatgg tttgaaattt tgcatatatc ctgtattgtc
     2581 taataattgt tccatatttg acatcccact taagaccatc ataacattat ctttacttgc
     2641 tgcaaaacgt attgcccaag atgaaattga catatctgga ttgtagtcat tcaataattt
     2701 ctctgctttt tctggtatct ttgcaagtgt acctcctttt actggctcca taacaattac
     2761 aggcttatta tgcttctctg ccacctcgta gcattttcta gattgtatgc tttcattatc
     2821 ccaatctaaa taattaatct gtaattgcac gaagtctact tctggatgct cactcaagac
     2881 tttatccaat agttctgcac tatcatgaaa agaaaaacca atattcttta tttttccttc
     2941 ttttttcttt tcttcaataa acttaaagct atcaaatttt ttagctactt cataatgtga
     3001 cacgcctata ttgtgtaaaa gatagtagtc aaaatagtct acaccacatt tttctaattg
     3061 ctcattaaaa atggtttctt gctcttcttt tttctttaaa gccattaatg gaagttttgt
     3121 agctatagta aaactctctc ttttgtgtct ttttactaga gattctctaa gtgctatctc
     3181 actttttccc atgtgataca cataggctgt atcaaaatat gtaaatcccc tttctaaaaa
     3241 agtgtctacc attttattta aatgttccat atcaatagtt gtaacatcat tttcatcttt
     3301 tattggtaaa cgcattaatc caaatcctaa tttttttgcc tccattgttt tacttccccc
     3361 tttaaaactt gtttgttttt tctctctact ttaactattc ttacaagtat ataattcttg
     3421 tataattata ctttactact tggagttaac ttcaagtcaa tagatttttt tatttttaag
     3481 acaaatatta taaaaataca gttctttaat attaatccat tgcatgtttc tttcaccctc
     3541 caattctcaa acttcacaaa ctccaacatg caaagaaata tatactttaa gaaattcagt
     3601 ccattaatat tactacaaat actttttaga tatgtttaaa aagaaatttc acctaaaata
     3661 tttataaaag atttagaaat aggtcttgtg tctattttaa aatatataaa acagtggtaa
     3721 agacttattt ataagataaa aaatgaatag tgtataccct ttttaaaaag ttttttcctt
     3781 tttatatttg tgatacaatg ttaaatataa aaaggaaaat tttgtattaa agaaatctta
     3841 tttgttgatt ttgtaacttc aaactcagta tcaatttgat tttaagatta tattttatta
     3901 agtatgctaa ataacaaatt tttaaagatt gtgaggtaaa aatatgaata ataaaaattt
     3961 agctatatta atatcaggta tgatgttttt ttctagttct tcagcaattt ttgcagacaa
     4021 tactacaaag caagaaagat taataggtaa aacaagatat gaaacagcag tagaagtaag
     4081 taaattggga tgggttcaat caaaaacagc cattatagtt aatggtaatt ctattcaaag
     4141 tgcgctttgt gcaaatccat ttgctaagct aaagaatgca cctatacttt tagtaaacaa
     4201 caacagtatt gaaaattcaa ctaaggctga gcttaaaagg cttggtgtag ataatgtgta
     4261 cattgtagat agtggaaata gcatttcttc taaagtagaa aatgaaataa aatcacttaa
     4321 cataaaaata aataaaatag taggaaacaa tatatataaa atgtctacaa atgtacttaa
     4381 agaaatagat aagataaaaa aggtagaaaa tgttgcagtt gtaaatggac taaagggatt
     4441 atctgatgca gttagtatag cttctccagc agcaatacat aatatgccta taatattagt
     4501 atcagatgaa aacagcaatg atagtagtat aaatttctta aacaataggt ctataaaaaa
     4561 atcttacata gttggaggaa ataagatttt atcagacaat acagtaaaga aatatccaaa
     4621 cagagaaaga atcagtggtt cagatagaaa caatactaat gcaaaaatta ttgataaatt
     4681 ttataaagat aaaaaaataa ataatgtatt tgtagctaaa gatggtatta accaagaaaa
     4741 tgcattagta gattcattat caataggagt attagctgct aaagaaaatt ctcctataat
     4801 gatatctaat ggtagccttg taaatagtca aaaggaattt attaaaaata aagatataga
     4861 tgaaataaca caagtaggag gaggatataa tcaaaaagca tttgaagata ttttaaaact
     4921 aaaaaatgtg aacataagaa ataaaactaa aaaaatatct ttaatacagg ctaaagaaat
     4981 attaaaacaa gctcttccag gaagcgatat tttagtttgt tattatgatg aagatgataa
     5041 tgaatatgac tgcaaagtta taaaggataa tatcctatat gagatagaaa ttgatgccta
     5101 ctctggtaaa ataatagaaa ttgaaaaaga tgatgactta aatgataata atttaggtac
     5161 acaaaatata tcattggata aagctaagtc tatagcatta gatgctgttc ctaatggtaa
     5221 tttaatctat tgtaaatatg atgctgaaga taacgaatat gaggctaaag taataaaaaa
     5281 taagaaaaca tatgaattag agataagtgc atttgatgga agaattgtag atattgacat
     5341 ggacaatgat tctgatgatg actttgatga taatgataag aataactcta atactaatga
     5401 caacagttct attattagtg ctgaaaaagc taaatcaata atgctagata aagtgcctgg
     5461 tgggaaaata gtaaaattta agtatgatga agatgacaaa gaatatgaag gtgaaataat
     5521 aaaaggaaaa attgaatatg agataacaat taatgcaatt actggtaaaa tattagaatt
     5581 tgaaatagat gattaataaa aaatatatat tctattaatt attacaagca ataaagccat
     5641 aaatattgaa tttttcaata tttatggctt tattttattt tttttaattt aaatctacat
     5701 tatgccatta tatatctatg cactctttta aattaaaatc ttgtccatca tcccaaacct
     5761 aataaagtta gattttaaac aatatattgt gatataagta atttgtgtag aattattgcg
     5821 tgatttactc atgaagaaac aataaggtta atatataatt tgactatatt aagacgaaag
     5881 gagacatata caatgaatac aatattaaaa gtaaataaca taactaaaac ttataataaa
     5941 gggcaacagc cgagtttaga taaggtatcc tttaatgtat cagaaggtga atttataggt
     6001 ataatgggag cttctggctc tgggaaaaca actctgctta atgtattatc tacaattgat
     6061 aaaccaacaa gtggagatat tttcataaat aatataaatt tgaaatcgct taatgacact
     6121 tgtgctgctg attttcgtaa ggataattta ggctttatct ttcaagagta ttttttacta
     6181 gatagtctta caattttcga gaatatttca gtacctctta cacttcagaa actcaatcca
     6241 gataaaatag aaaacatggt aaagagtcta gctgaacgat ttgacatagc ttctcaatta
     6301 aataaatacc caaatgaatt atctggtggg caaaggcaga gagcttcagc agttagagca
     6361 atagtaaaac atcctagtat tctctttgca gatgaaccaa caggagcttt ggattctaat
     6421 tcagctactg aactactcca ctcactacag gaagcaaatg aggagttgaa aacaacaatt
     6481 ttaatggtaa cacatgatgc ctatgcagca agttttgcaa atagaatttt gatttttaaa
     6541 gatggtcata tcattaaaga attaaataaa caaccgaata atagacatac attttatgag
     6601 ttgattattc aagaaattgc aaaattggat actaaaagat aggaggataa tttaatgaac
     6661 attttttata tggcaataaa gaatcttaaa aaaaactttt cattctattc tctatatcta
     6721 ctttcagtct cttttgttat tactgtattt ttcgcattta cttcattctc tgttaataag
     6781 ataatgctag aaaaaatttc aactgatggt agagttgaat ctatgtgtaa tgttatttcg
     6841 atatttttga tggcatttgt catcttctat atggcatact caaatcgatt cttcttgaga
     6901 cgtcgtacaa aagaattagg catatatgct ttacttggct atcgtaagtt tacaatatta
     6961 tcgctactta cctttgaaaa tgtattaatt tgtagtggag cttttattac tggattggta
     7021 ttaggagcat tgtcacataa gggtattgtc tttggaatta cagcactact taatttatca
     7081 attgataatt cacagatacc attttttaat ataaatgcaa taaacaaaac aattatcttt
     7141 atagcattag ttgttttagt cttagctata tcaaatggta aatttcttta taagacttca
     7201 cttgtaaaat tgataagaca tgaaaaaagt gcagaaaaaa aattaaagtt cagaaaaatt
     7261 ccagccttta tgggatttat tatgatttta agtgggtatt gtttatcact agatattctt
     7321 cgtggagcta aatcagtatg ggtaagtatt ggattttatc aaatgggctt gataactatg
     7381 attatcattg taattggtac agttctcttt atcaattcct ttcttccata tattatgcaa
     7441 aagagtaaaa aaaataaacg tactttctac acagatatga agataatagc tattccaaac
     7501 tttatctatc gtatacgttc aaactctaaa actctaatta tgcttacatt actctctgct
     7561 actactttga cagtttcaag tgttatggca ctgtccttat actaccctat tgcatctgtt
     7621 tcgcgtattg caccttcaga gatagaattt agagtagaaa aaaataaaca aatagaagat
     7681 gtaaaacaaa ttgtcagtca atatacgtct aatacagatg atattatttt tacacagaca
     7741 aatatttata aggtaaaatc ttcatcaaaa gaccttccat ttgaatatga aattggtaca
     7801 tctaagaata atttatctaa tagtagttct tctaaagaag ttatttcaag agaatcagga
     7861 tttgaatgta tatctttttc tgattattat actttattaa aggcacaaaa tcgaacaaag
     7921 ctactaaaac aacttacccc tttaagggat aatgaatgta ttttaatgaa atatcaacca
     7981 aataatgatg gatatagtga aaatggaacg atttatccat tacttattaa tggtaaagaa
     8041 acacctgtag ctgtaaggga aacatcatta aacaatgcta tttcttttgc caatagcgtt
     8101 ggaacactta tcgttaatga tagtatatat gagcaaatag cacaaaataa attaccaatg
     8161 acaagcattt taagtattaa tggtaaatca attaaaaata atgaaaattt atatagtaat
     8221 atttcagatt atttagatgg aagcccttat ttacaaggat attctcacag gat
//
LOCUS       DAEPLF010000152         8175 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEPLF010000152 DAEPLF010000000
VERSION     DAEPLF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.152"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(52..1398)
                     /locus_tag="KR314_002734"
     CDS             complement(52..1398)
                     /locus_tag="KR314_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH0329104.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIRKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            complement(1580..2173)
                     /locus_tag="KR314_002735"
     CDS             complement(1580..2173)
                     /locus_tag="KR314_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBH0329105.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(2238..3707)
                     /locus_tag="KR314_002736"
     CDS             complement(2238..3707)
                     /locus_tag="KR314_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBH0329106.1"
                     /translation="MSNVNKKLVIAIGAVALVAIIFIGIMCMQFKGEKIAKNTYVNGV
                     DIGKLTKSQAKQELAKKYKLENVEFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SSFFGNLSKTISTNFGKKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLTNIDTNLGTY
                     STIFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            complement(3822..5213)
                     /locus_tag="KR314_002737"
     CDS             complement(3822..5213)
                     /locus_tag="KR314_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBH0329107.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKEDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            complement(5379..5627)
                     /locus_tag="KR314_002738"
     CDS             complement(5379..5627)
                     /locus_tag="KR314_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329108.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSTNKKQKSHSRDIKRKIS"
     gene            5893..8022
                     /locus_tag="KR314_002739"
     CDS             5893..8022
                     /locus_tag="KR314_002739"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBH0329109.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
BASE COUNT         2656 a         1306 c          902 g         3311 t
ORIGIN      
        1 aatttaataa aatacatact tggttgtttc aaaaaactta actatataaa cctatttttc
       61 acttaagatt tcttttattt ttgaattgtt atacatactg tctggaactt cacttctcaa
      121 actataaaaa gtctgttgtg cattttttaa atcgccatta tcataataaa gcatacccaa
      181 ctcgtaatat gaatcatcat aatactggtc atttttagta taagtattta tatatttttt
      241 gtaatatttt atagcttcat ccttatttcc taataattta tttgttattg ctaattgata
      301 tattgactca gaaatatatt ttttagtttt accagaagaa actacacttt taaaattatc
      361 tatggctttt tcatattctt tgtttttctt taaatttaga gcttgataat ataaatcttc
      421 ctcatcagct acagttatct ctctttctgg ttctttattt ttaacctcat ttaattcctt
      481 attcgttttt tctaattttt tactcgtttc tcctaacttt ttatcagttt cacttaactt
      541 ttcctcttta ctacttattt ggttattcaa gttttctatt ctctttttgc tttgaatcaa
      601 atatatcccc atacctatta aaacacagcc aactataata tatggtaaaa tacttttctt
      661 tctttttact tttcttattt cttccttttt acttgtatct tctttttctt cttcattttt
      721 ttcaaataca ggattatctt tatctagctc tttaagtttg tctaaatatc cttctctctt
      781 ttttacatta cctagtttat catataacaa agataagatt acatatggct ctattagctc
      841 aggctcttct tcaattatgt tctctaaaat atgtattgac tcataattta attcttcatt
      901 tataaatctt atagaatgat tgtatcttcc taaaaaagtt ttaaaatctt cagatgaaat
      961 tagatttaca tattttctag atatatgatt attctcataa cataaagcag tataaaaact
     1021 ctcaaatgat ttagcaaaat cacattttaa taactttaaa agacctctaa gatttagtat
     1081 atcaatatct ttaggattta tctccagagc tttattaacc atattaatgg cactagttat
     1141 ataattttta tatgctaaat ccaatgcatt gttatatctt ttatatgatt ctaattttaa
     1201 ctccttagac gttgtgtctg actttgaatt taaagctcct tgcaagttta atagagtttc
     1261 ttctttataa tttgtagtca cataactctg ttttaatgct tttaatgttt catcaccgtc
     1321 ttcttcttct atattaaaaa aagtcaaatc acttttatta tcttctatca aagatatatc
     1381 taaaattttt ctaggcatgc tttctatgtc ttgttttata tctttaacta aaataatact
     1441 cacctccact ctaaaatata tatttaagat aacatattta atatgtcaag tcaaaactat
     1501 acatattttt ccaccattct cataataaaa aaggtaatcc tcttcttttc ataaaaaggt
     1561 tacctctttt tcaatactat taacttttaa tgttgaattt ttttactttt ctatagagtg
     1621 tagctatacc tatatttaat gcctcagcta cttttttcat accttctgta ttacttccat
     1681 atttctttat ggcattttct atagcttccc tctcaaggtc atctatagtc ttaacttctt
     1741 gtttataaag ttcatctagt ttaaaagata ttaaatcaga catactcctt acaaaagcag
     1801 ttaactcctt atcttttgaa agtatctttt ctttttgctc ttcattaaac gcagctatac
     1861 ccaagattcc aagtatatgt tctcccattt taacaggagt acatatatca gctaattctt
     1921 tacaattttc tctatttgca catttattac agttctcttc tatttcctta tttataacat
     1981 aggattttcc atcttttaaa acaagagagt acactgactt ctctgatatt gtctctccta
     2041 ttttacctct gaatattcct gtacctgcta tcctagttaa atcttttgta actaaagtaa
     2101 catctacatc caatacatta gaaatattct cgcaaatact ttgaacgtaa ttcgttatat
     2161 tatttaaatt caaagtaatc cctcctattt agatttatat tttttattac tctacataaa
     2221 tcctattctt gagaacttta agcttgcggt tgtggttgtg tctcattagg cttattatca
     2281 gttggtggtg tttctggttt ttgttgatta tttggatttt gattgtttgt attttgatta
     2341 ttcttatttt cgtctgtatt tggtttttcc tctggttttg gttccatagt cccaactaat
     2401 ataatacctt ccttcttagg ataatatgaa ttagctattt tttcagtttt aattttttta
     2461 ccatttgcat cattaaatgt tctatatgtt gatacagaat atgcatttct tccacttttg
     2521 tctacttttt ctttaccttt aggcattgta gggtcattaa ctcttttcat tgtagtttgt
     2581 gaaacgccat ctaattttgt tgatatcgtt atattttgtt tatcttctgc acttccatat
     2641 atattgcaaa caatttgatt tcctgaaacg taatttttta tgtaaactgg atgctttaga
     2701 ttatttttaa ataagaaatc tattccacta tctgcaacag ttgcatctct tccttttgga
     2761 acataacttg atggtatagt atgatttttt acattaacaa tttctaaacc tgcgtataac
     2821 acagaattgt ataacgtaga tgaaacttga caaactcctc caccataatc ttcttccatc
     2881 tcaccttcca ttattacagg tgcactttta tatccatttt ccttacttct tttccctgta
     2941 tgtttattaa atgagaaagt ttctcctggc ataagtaata cattacttat attatctgtt
     3001 gctaatttta tgtttatact tctatttatt tgagaagttt taaatattgt tgaatacgta
     3061 cctaaatttg tatctatatt agtaagttgc tcttttttta cttttgctgg agtagctttt
     3121 actactaact catttttata gttacctttt ttagtttgat tatcaaaatt ctccatactc
     3181 ttagaaatat ccattttaag accatcagaa tctggtataa ccttgacttt ttcaccactt
     3241 atatcaagtg tagcatcttt tacatctaca tctatttctt tagctatttt ttccatttct
     3301 gcttttaatt tatttttatc ataatttatc acaactacta agttgctttt tttgccaaaa
     3361 tttgttgaaa tagtttttga tagattgcca aagaaactac tctttctatt taaattatat
     3421 gcgttttcta ctgttttatc cagatcagta gataaattta aatctttgct ctttactttc
     3481 caacttttat cattgtaatt aaactcaaca ttctctaatt tgtatttttt agctaattcc
     3541 tgttttgctt gcgatttagt taattttcca atatctactc cattaacata tgtgtttttt
     3601 gctattttct cccctttaaa ttgcatgcac attatgccta taaaaattat tgctactaat
     3661 gctacagctc caatagctat aactagcttt ttgttcacat tactcatccc atacttctcc
     3721 ttacaataaa attatttata tatccctatt attatattac tacataatgc gccttaaaac
     3781 cccaaaaact ttttaattat atattaattt aatattatta attaatattc atttagtcca
     3841 ttttctgata tggcatatat tttttcattg taaatattaa tagcataaac ttctttgcct
     3901 ctaaataagc ctttatttac tacagcataa taataaatat ttttatattt tccaaacaat
     3961 tcttctaaac tctttttact ttttacatat ctatctgctt tctctatatc taatatctca
     4021 atctttttat acttattttc tttttcatca agagtattag ttaacacaaa cttagcttta
     4081 tcttttgtaa taccaaattg ataacttcca tcttctaata ttctaaagta tccatcacca
     4141 taatctactt tgtaatcttt tttaccatct ttatacgaat ataaatcttt agtttttgca
     4201 cttaccaaaa cttcatttat taatttatca tactcatata cttcagcata atagtattta
     4261 ttatttttta tcatttgtgg aagtgaattt atagatagat ttgccttgtc tttgccactt
     4321 tttttagcta ttacatttat aatgtcatca gatgttaagg tatacaatga taaattcttt
     4381 ttatattttt tttctaaact ctgtaccttt tcatcatctg aataatcttc ttttaaatac
     4441 tcaatatatt gtaatgctct ttcataatct ccagaagtat ttgcctcttc agctctactt
     4501 atatagtaat catacatttc ttcaatacat tgttctttcc cattttgagc catactgtaa
     4561 tacttttcat cttcttttaa tactttatta tacttttcaa tagcttcata atacttatat
     4621 gctttttggt cttttactgc tgagtcataa acatctctag actctttgat tgtttctata
     4681 ttctgtttgt atacatctag gttactaccc ataccattta atattgaaac agtacttatg
     4741 tagttaatgg caatatcata agtagtattt tcttctttat acatttctct aactctatca
     4801 gcttgagcta ataagtcatc cacatctata cttattttgt ttaattcttt cacagtattt
     4861 attaatccaa taaaactttc ttgacttatg tctccatcta aatatttatc accactattt
     4921 aatagcaatt tatttatttt tttagaaaca gacttattaa acctttccat ttttgaatca
     4981 gaaaactttt tttctatact tgtgtagtaa tcaacagcta tagaatactg tcttttctct
     5041 ataatcttta acaactcatt agcttcattt cctacactaa actcgttact acttaagaaa
     5101 aatactatta tcaaagcaat tacacctaat gttgataatg aaatcgcaac tatagatttc
     5161 cttttttcat caataaaatc tacagccttc tcaaataaat ctctaaaatt cattatagtt
     5221 ccctctccat ttaataatct ttatatttta tggtaaacta aatttcttat tggttattat
     5281 atcataattc ttgaaaaaaa ataaaaacac aagttaaatc gtgtttttat tttacctatc
     5341 aatcattatt ttatacataa atatttcctt tacataaatt atgatatctt tctttttata
     5401 tccctagaat gactcttttg ttttttatta gtactgacag ttttcgttct tgtcttgtgt
     5461 atagctttgc tatcaaatat ttttctagca ttgcataaaa caattccaat tgttaaacaa
     5521 actgtaagca cacttcccat caaaaagtaa taatccaatc ctatatgtac tcccataact
     5581 gaatttatta acactaatgc aaaagtcatt gcaaaacata tcatcatgga gataactaaa
     5641 attcttagaa tttttttcaa tccaatcacc tcgtataatc actaaaattg acactacccc
     5701 ttatccatta ctataccaaa ttagtaaaaa aatagcaatt actattgtca tttatattag
     5761 gaaaatatta aatcttttta ataaatatct ttttttgaca taaggattac ttaactttta
     5821 ataaaatagt aacactaata tgtattaatt aatagaataa aataaatgat aatttattga
     5881 ggggtgattt acatgccaaa tttgccaagt ttagggtcaa aggctcctga ttttaaagcc
     5941 aatacaacaa atggtcctat tagactctct gactataagg gtaattggat tgttttattt
     6001 tcacatcctg gtgattttac accagtttgt actacagaat ttttatgttt tgctaaatat
     6061 tatgacgaat ttaaaaaaag aaatacagaa ctaattggtc taagtgttga tagtaacagt
     6121 tcacatttag cttggatgta taatatttct ttacttacag gtgtagaaat tccatttcct
     6181 attatagaag atagagatat gagaattgcc aagttatacg gcatgatatc aaaaccaatg
     6241 agtgatacat caactgttcg ctctgtattt attatagata ataatcaaat tctaagaacg
     6301 attctttatt atccactaac tacaggaaga aatattccag aaatacttag aatagtagat
     6361 gcacttcaga ctagtgatag agataatata gttactcctg caaactggtt tcctggaatg
     6421 ccagtgattt taccttatcc taaaaactat aaggaattaa aaaatagagt taacagttgt
     6481 aataagaaat attcatgtat ggactggtac ttatgttttg taccagataa ttataatgat
     6541 gaagaagtga gcaagaaaat tgataatact tgtagctgga aaaaagaaca tactaaaaac
     6601 attgaaaatg aatgtaactg tgaacatgaa catcatgact acctgaacaa agctcttgac
     6661 tgtaaacaag aacacaagac tgatattaaa gatgattgca accatgagaa aaaacatact
     6721 aaaaatacta acaaagttca caactccaaa caagataagt ttaaagataa gtcttgtgat
     6781 gaaatgaatt ttaactatga taaagatgaa tcttgcgaca aaataaattc tagctataac
     6841 aaagaagata gtagttatga agatttctat aaacataatt ataaaaacta cgattatact
     6901 agcgaaaaaa atactaaaaa aatagctatg aaaactttaa aagattcaaa aaaattagtt
     6961 agaccacaaa taacagaccc atacaatcca atagttgaaa atgcaaactg tccagatata
     7021 aatccaattg tagcagaata tgttcttgga aatccaacta atgtagatgc tcaactatta
     7081 gatgcagtta tatttgcttt tgctgagata gaccagtctg gaaatttgtt tattccttat
     7141 cctagatttt taaatcaatt acttgctctt aaaggtgaaa aacctagctt aaaagtaatt
     7201 gtagctattg gaggttgggg agctgaagga ttctctgatg cagcattaac acctacatct
     7261 agatataatt ttgcaagaca ggtcaatcag atgataaatg aatatgcttt agatggaata
     7321 gatatagact gggaatatcc tggaagtagt gcatctggaa taacatcaag acctcaagat
     7381 agagaaaact ttacactctt actaactgcc ataagagatg ttataggaga tgataaatgg
     7441 cttagtgtag ctggaacagg agatagagga tatataaatt caagtgctga aatagataaa
     7501 atagctccta taatagatta ttttaatctt atgagttatg attttacagc aggtgaaaca
     7561 ggcccaaatg gtagaaaaca tcaagcaaat ctttttgatt cagacttatc tttgccagga
     7621 tatagtgttg atgcaatggt gagaaatctt gagaatgctg gaatgccttc tgaaaaaatc
     7681 cttctcggta taccatttta tggaagatta ggtgccacta taacaagaac ttatgatgag
     7741 cttagaaggg attatataaa taaaaatgga tatgaatata gatttgataa tactgctcaa
     7801 gttccgtatt tagttaagga tggagatttt gcaatgtcat atgatgatgc tttatcaata
     7861 ttcttaaaaa ctcaatatgt tcttagaaat tgtctaggtg gtgtattctc atggacatca
     7921 acttatgacc aagcaaatat actggctaga accatgtcta ttggtataaa tgaccctgaa
     7981 gtattaaaag aagaacttga aggtatttat gggcaattct aatagaatgt aggaagattt
     8041 tatagttata taaaaagcag attttattat attaaataag ttgtatctaa ttaatactat
     8101 tattaattgt ttataaatta gatacttatt ttttcataat acaatctgct tttttaatca
     8161 aaatttattt aatta
//
LOCUS       DAEPLF010000153         8169 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEPLF010000153 DAEPLF010000000
VERSION     DAEPLF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8169
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.153"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..1455
                     /locus_tag="KR314_002740"
     CDS             <1..1455
                     /locus_tag="KR314_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBH0329110.1"
                     /translation="ILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIF
                     EKCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQ
                     HFKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYG
                     QTLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIA
                     LIVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNK
                     EKSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMEN
                     SVNWYFQTLDKKTGLKNIQTYLKQIGYGNYDVSGGISNFWLESSLKISPIEQVELLES
                     FYTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENT
                     YFFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(1810..3684)
                     /locus_tag="KR314_002741"
     CDS             complement(1810..3684)
                     /locus_tag="KR314_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0329111.1"
                     /translation="MNKYNKIANELIEIIGENNIISITHCATRLRVMVKDREIINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEVEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIGLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(3689..5353)
                     /locus_tag="KR314_002742"
     CDS             complement(3689..5353)
                     /locus_tag="KR314_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBH0329112.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDATPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSKYSDCLIYGSIEPIDIESDNVIAYRRQLDLVVIDCY
                     YNFSDKLVEIKTEEKYDIVFQNLDDINLLNKTVSLKPFQAVLLKVR"
     gene            complement(5367..6188)
                     /locus_tag="KR314_002743"
     CDS             complement(5367..6188)
                     /locus_tag="KR314_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0329113.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSIGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDVHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(6287..7051)
                     /locus_tag="KR314_002744"
     CDS             complement(6287..7051)
                     /locus_tag="KR314_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0329114.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVVKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDTSTIIDFAKEMRMKGTYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(7048..7797)
                     /locus_tag="KR314_002745"
     CDS             complement(7048..7797)
                     /locus_tag="KR314_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0329115.1"
                     /translation="MRLESLVNQSYNSLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKSGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
BASE COUNT         2879 a         1376 c          885 g         3029 t
ORIGIN      
        1 attttattac taatatcatg gataattgga attttaatca tgatagctat tactattcat
       61 tgcaatatta aaataaaaaa tatgaaaaaa tcaatacaaa ttttaagaga tgaggagata
      121 gtatttatat ttgaaaagtg taaaaaagat attggtataa ataaggattt tatactatgc
      181 aaatcaccta ttataaatac acctattaca tttggatact ttaaaccaca cattatatta
      241 ccagataagt ctatatctaa attatcttta aaagatataa aatatatctt acttcatgaa
      301 ttacaacact ttaaaaataa agatatactg ataaattata taatgtgctt ccttcaaata
      361 ttatattggt ttaatcctct tgtatggtat gcatttaaag aaatgcgtat agaccgtgaa
      421 attgcatgtg atatttcagt tttaaaaaag cttgataaag atagtcatat agaatatgga
      481 caaactctaa taaactttgt ggataatatt tcaagaccat caaatcttac acttacatca
      541 gatattggtg ggtcaaaaaa acaaataaaa aagcgactat tgaagataat ggattttcat
      601 aatgaatcaa atacgttaaa attcaaaagc attttaattt ttacattgat agctttaatt
      661 gtatttggaa atattcccaa actatctatt atggcagata tcaacgaaaa atatagacct
      721 acaaatgaac aaactgtgta cgaggactta agtcagtatt ttaaaggttt tgatggaagt
      781 tttgttatgt atgatttaaa atccaagcaa tatcatattt acaataaaga aaaaagtaaa
      841 acaagaattt ctccaaattc tacttataag atttatagtg ccttattggg attggaaaat
      901 gggataataa caagaaataa ttcacatatc ccttgggatg gaaagcatca ttctaatagt
      961 tcttggaatg aagaccaaga cttattttct gctatggaaa actccgtaaa ctggtacttt
     1021 cagactctag acaaaaaaac agggctaaaa aacatacaaa cttatcttaa gcaaattgga
     1081 tatggcaatt atgatgtttc aggtggtatc tcaaactttt ggctagaatc ttcattaaaa
     1141 atatcaccta ttgaacaggt agaattactt gaaagttttt atacaaataa gtttaaattt
     1201 gaagataaaa atatagaaac agtaaaagat tcattgttaa tatcaaaaaa ggatggggta
     1261 tcactttttg gtaaaacagg cactgctaat ataaatggaa aagatgtaaa tggttggttt
     1321 ataggttatg tggaaaaaaa tgaaaatact tatttctttg ctacaaatat tcaaagcaaa
     1381 gattattcta atggtagttc agcatctaag ataacattat ccatattaaa tgacaaaaaa
     1441 atctataatc cttaatatat ttactgtaca ttctaaaaaa ttcacataat tttcatatta
     1501 attatcgatt aaattcatat acaaggctta ttatataaac atagaagata gtaaaactag
     1561 cttaaaaaca taatataata ccctatacaa atagtggcaa ctctccctca catcctcaat
     1621 tgttgccact atttaatttt ttgtatgaag ttaaatatat tttcatcatt tcaacaattt
     1681 gggtgcagta caatctctat tttgagacaa cctctttaat tgttttagag tttatgaatt
     1741 atttctcata ctttaaaaag attgaattac tcttgataat cttcttttgt agtcattaca
     1801 tatcacctac tactcaacac atattctttg acctattttt acatccttgt ttatatccaa
     1861 aatatttacc ttcttaccat ctggaaacaa taccattgtc aaagtagact ttcctaattc
     1921 atgtattctc tttaaatcaa cattaacaag aacatctccc tgtttaacca tatctccttg
     1981 tttaacagca atatcaaagc catctccatt taatgcaact gtatcaattc caacatgaat
     2041 taatacttct ccattagttc ctttaatccc aaatgcatgt ccacttgaaa atactgtttc
     2101 taatttacct gaaataggag aaacaatttt tccatctata atatcaatag caaaaccatc
     2161 acctaacatt tttttagaaa atgtttcatc tggtacattt tctaatgcaa caattttccc
     2221 cttagctgga gacacaaagt tttcattatt ttcattaaac actaattctt cgtcttcatc
     2281 ttcatttata gcttctttag gcactccaaa taaacatgtt acaataaagc agaatgcaac
     2341 cacaaatatt gatactaata agtatgtgat taaattatat ccatcataga tatacattaa
     2401 ataagaaggt aaaactgcta atccatatga attagattga atatgtaaaa ttgataatat
     2461 tgctgctcct aaacctgaag atgcacacat aataatcaaa ggctttaaat tatatcgaag
     2521 ctgaatccca aataaaacag gctcacttat accaaaaagt tgagatagca ttgctccaac
     2581 tgcagtttgt ttaacttgat tatttttaga ttttttcata aatgctagac atgttccaat
     2641 attcgcaaat ccatacattg cacaaagagt aataagtgca ttaaatcctg tatttgctaa
     2701 tagtgatgtt tcaatcatta cataagtatg atgtaagcca gtaataacag ctaatggata
     2761 tgtaaatcca ataataaatc ctccaattcc taaaggtaaa ccaatcaata aactgatgat
     2821 atcaaccatt tttaattcta ttgtatgcat tataggccct acacctaaca aaataactaa
     2881 cattgttatt agcataacaa agaatggtgt gaaaataagg tctaatacat taggcatatg
     2941 tttacggaac catttttcaa gattagctcc aataaatgct gtaacaatag ctgttaaaac
     3001 acttccttga cagcctacaa taggaataaa tccaaatgcc ataattgctt caacttcatt
     3061 tgatgggtca gcaacagaat atgcattagg aagtattgga gaaactaaca taagtccaat
     3121 aacaagtcca ataacaggtg ttcctccaaa taccttaaat gctgaccata caataattgc
     3181 tggtaaaaaa gcaaatgctg tttctgtaag aaccgaaaca agtgtctgaa tatacaatgg
     3241 aatatttgca gaagacattc caaataatcc aagtacatta tcattaaaca aacaaccctt
     3301 taatcccaag aataatcctg ttgccgcaat aactggaata ataggaacaa atatatctgc
     3361 tagagttctc ataactttct taaatccagt ttcattattt ttaactaatt catcttgttc
     3421 tttttttgat aaagttttca atcccatttt ttcaacttct gcataaactt tattgactat
     3481 tcccgttcct agaataattt gatactgtcc agaagtaaag aaaacacctt taacctcatt
     3541 aaccttttcc actttcttat cattaataat ttctctatct ttaaccatca cccttaatct
     3601 tgtggcacaa tgtgtaatag aaataatatt attttcacca ataatttcaa taagctcatt
     3661 tgctatttta ttatatttat tcatttttct acctcacttt caataatact gcttgaaatg
     3721 gtttcaaact cactgtttta tttagtagat ttatatcatc taaattttgg aatacaatat
     3781 catatttttc ttctgttttt atttctacaa gtttatcaga aaaattataa taacaatcaa
     3841 taactaccaa atctaattgc cttctatacg caataacatt gtcactttct atatcaatag
     3901 gttctattga accataaatc aaacaatcag aatacttact atcttgtctc aactttatca
     3961 tctttttata aaattctaaa atactttctg aattgttaat ctgactctgt gcattaatat
     4021 ctttataatt ttcaataacc tttaaccatg gtgttgcatc tgaaaaacct gcattttctt
     4081 tatctgccca tggaaatggc gttcttgcat tatctcggct tcttaaattc acaaattcta
     4141 acgcttcttc ttctgagtaa ccttcatcta ttgaacgata gtattggtca atgctagata
     4201 aatcattaaa atcatttatg gtatctcttt taaaatttgt cattcctaat tcctgtccct
     4261 gataaataaa tggtgttcct cttaaaaaga aatacattgc tcccaacatt tttactgcat
     4321 catcattcct ttcgtattgc aaaagatact ttgttgtagc tcttggttgg tcatgatttt
     4381 caataaaatt agctgcccat ccatgttcct gaacatacat ttggctatct aaaatgcatt
     4441 ttcttaaatc tggaattgtc catgaaattc ttttaaacca ctcactacca gaagcaacat
     4501 ctaagtcagc atgtttaaag tcaaagacca ttgaaaagaa accatcttct ccaataaaat
     4561 cacctagttg gtcatagcta atacctgctg cttcagcaac agtcatacaa tcatattttt
     4621 taaacgtatt ttcttttaat tcataaagca ttttttctat tccaggctga tttctaactg
     4681 tttttgtaca tttaacaaga ccatcaacac catcactttc taaactttga aaacttaaat
     4741 tctttttgat aaatgtaata gcatcaactc taaatcctgc cactcccatt tgagaccagg
     4801 tattaatcat ttggtaaagt tcacttctta atattggatt ttcccaatta aggtcaggtt
     4861 gttttttacc aaaagaatga aaataatatt cgtctctatt ctctaccttt tcccaaacac
     4921 ttccaccaaa aacagacctc cagttatttg gtaaatcgtt tcctttttta aaaatataat
     4981 aatcatgata aggactttct ggatttttta atgcttcttg aaaccaagga tgttcatcac
     5041 ttgtatgatt aagaactaaa tctaatataa ttttgattcc tctatcttta ctttctttaa
     5101 ttaactcttt taaatcttcc atacttccaa attcacttgc aatatctaaa taatcactaa
     5161 tatcataccc attatcgtcc ataggtgatt taaaaattgg acataaccac aatgatgtaa
     5221 tacctaaatc ttttaaataa tctaaacgag aaataattcc ctgtaaatct ccaattccat
     5281 cattattact atcttgaaag cttcttggat aaatttgata aacgatttct ttttgccacc
     5341 atttcttttc cataaattac tcctttttat atgatatttc ttctttttaa ttctttataa
     5401 attccattat cgaatatgtc ctcacatata gatgtcgcta tatctttaag ttgttgatga
     5461 ctatttttca ttgcaattgt aacgtctgat gcttgaaaca tgacaatatc attttgacca
     5521 tcaccaaaac atatggtttc tttttgagtt acccctaatc tttcttgtaa tctcttaatt
     5581 gctttgccct tatgaaaatc tttttgaata atttcataat actgactaga aatatctctc
     5641 tgtgctaatt ccattttatc ttgtagtata tctttcactt cgtcaaatac cttttcattt
     5701 gaccataaac aaattttatg tacatcttgt gacttatact cttcaatatt attttcatat
     5761 gttatttttt cttggatatg ttgtttattt atacaagaat tagttccttt caactgattc
     5821 attgtttcaa atatttcttt ggctttttga ttcatgaaaa ctttttcctg actctcaatc
     5881 gaaaaagcaa cttctctctt ttttaacaaa caaacaactt cctttatcaa tctttgatta
     5941 aaagattgat tataaagtaa ttcaccatga tattgaatgt aattacctcc tcctgcaata
     6001 tatccatcaa cccctaatga taaaacatca tcttgtattg tacctatact tcttccagta
     6061 caaataacaa cactgcaatg attcttttga cataaacgta tagcgtgctt tgcagattct
     6121 gggattccat atacctcatc cctaagcgta ccatctatat caagtattaa taatttgtac
     6181 aatttcatct tcctttctta ctatcaaaat cataatagca ttcaaaaaaa aagatatcaa
     6241 tattttgata tcttttaaaa aatatgttca tttttagcac aaattttcac tttcggggaa
     6301 tatgttcact ttctaaatac atcttgtatt taatataaag cagttcaatt aaaataaaat
     6361 aaggtgttgt tacatcgtag tccagttgtt tatctacaga aatagatata gattgaatat
     6421 acaggttttc atcacataat cttgctaaag gattacttat catttttgta atagaaacca
     6481 tataagttcc tttcattctc atttctttag caaaatcaat aattgtagat gtatctccat
     6541 tcaatgagat aaagaaaatc atatcatctg tttttataaa atgattaagc gcctgaatca
     6601 tatcataacc ataaacatca tatataattt tccctgttgg taagaaaatc cttttcatct
     6661 ctttaacaac tctttcctga gaataacctt ctccaaagac gattatacga tttgcctttt
     6721 tcattctttc aaacataatc gaacaatcct tcttctctat atcatctatc accttattat
     6781 aacaatccat taattggtgt ttactttgat ttttatattc tacttctgtt tcatttaagc
     6841 gtataataac tcttaattca ctatatccag gtaattgtag tttttgagca aaacgagaaa
     6901 gtgcacttgt agatatatgg tatttgtctg caaattcatc tatagaaagt ttaatacaat
     6961 cttctttatg attaagaata cactcacaga tatacttatc atttgctgta aatgaattat
     7021 aatatttatt gaaaagtcct tcaattttca ttcttttctc cttctatact ctatgtaatt
     7081 catatgaagc aaatctaata gagcataaaa tgctcctacg acttcataag atatagcttg
     7141 taatgtatca agtttttgag tttcaacata cagattatac tcacaaagtg ttgcaattgt
     7201 attattatca agcctagtta aagaaatcgt atgtatatga ttgttgataa attttgcaat
     7261 ttcaattcct gattttgttt ctccagacaa agaaatgata ataaataaat cacttttttg
     7321 aaatgattct ctcataaatt caatttcacc tatatcaaat aagtcaataa cacatttacc
     7381 tactgaaaga aatattctct ttatttcttg agctaaactt ttctgttcat tacctgtccc
     7441 ataaatataa attgtttctg catcatatat tattttacaa atatcttgaa atgatatttt
     7501 ttgtaattca ttcattaata gtcgataatt ctcgcaaaca tcatgaaaat tattatcatt
     7561 attaacaaat tctagttgat tatttttaag taaatacttt aaatcagaga atgaatctaa
     7621 tccaattttt ctacaaagcc ttaataaagt agctctagaa acattacatt catttgcaat
     7681 tttttcacac gaaaaattac ggtattctct ttgacaaatc aatatttgtt taataataat
     7741 tttatcattt tcatttaaac tattataact ttggttaact aacgattcta atctcatttt
     7801 ttccactccc taaatattat tttaatatac attagttgct ttttctagtg aagagtctta
     7861 aataataagg ataaaataat catttgattt tatttttatt aaaataaaaa taacctgcta
     7921 aaagcaggca ccaaaaagta cttatccttt cgaacatgta ctttagatat tgctttcttc
     7981 ggaagcttca tctcttcata ctctaaaata gagttttttc taactcaaaa gtattttcca
     8041 tggtagaaat tactttaatt catctgccat ttatttttta tcttatttaa taattactat
     8101 tacttgaata tttacttctt taagtaaatt tcatagttaa caaagctaaa actattatta
     8161 tagatgtta
//
LOCUS       DAEPLF010000154         8169 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEPLF010000154 DAEPLF010000000
VERSION     DAEPLF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8169
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.154"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            362..1126
                     /locus_tag="KR314_002746"
     CDS             362..1126
                     /locus_tag="KR314_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HBH0329116.1"
                     /translation="MQIIDININNIDSRHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     AFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKGFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKI"
     misc_binding    1349..1562
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1816..2850
                     /locus_tag="KR314_002747"
     CDS             1816..2850
                     /locus_tag="KR314_002747"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBH0329117.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKNI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEVL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2892..4109
                     /gene="argJ"
                     /locus_tag="KR314_002748"
     CDS             2892..4109
                     /gene="argJ"
                     /locus_tag="KR314_002748"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBH0329118.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKENDVLVASTGVIGKPLNINLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            4124..4984
                     /gene="argB"
                     /locus_tag="KR314_002749"
     CDS             4124..4984
                     /gene="argB"
                     /locus_tag="KR314_002749"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBH0329119.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGMDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            5014..6201
                     /locus_tag="KR314_002750"
     CDS             5014..6201
                     /locus_tag="KR314_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBH0329120.1"
                     /translation="MNNNNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6310..7332
                     /gene="argF"
                     /locus_tag="KR314_002751"
     CDS             6310..7332
                     /gene="argF"
                     /locus_tag="KR314_002751"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBH0329121.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAYVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQKIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7459..8076
                     /locus_tag="KR314_002752"
     CDS             7459..8076
                     /locus_tag="KR314_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBH0329122.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     TVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
BASE COUNT         3323 a          775 c         1496 g         2575 t
ORIGIN      
        1 tctaatgcat ttttaatatc atcttctttt aattctctta ttaagtattc tttgaacatt
       61 ataaacaaca cctttattat tattttaatt ataattataa ttatatatct taaataaatt
      121 taattcaata caatttgttt tataaattta gaatattatg ataatatata gtttatatat
      181 agtgtggtta gacctagaat gtatttaatt atatatgcaa tttgataatt caaataatct
      241 aaaaataata ttatgtaaaa taaaaatata tatgacatga tgttgttgta ttcaaaatat
      301 taagataaaa atatatagaa tatatttaaa aattattatg taattagaaa aggagataag
      361 tatgcagatt atagatataa atataaataa tattgacagt agacatatat gctgttcaat
      421 ttctgaaaaa aaaggagaaa attgtgtttt ttctaaaaaa gcttggatga aaaataattt
      481 tgataaaggt ctagccttta aacgatttga tgtaagaggt aaagctttta tagaatatat
      541 tccagcagaa aatgcatggt gtcctattac tgcggacaat tatatattta ttaactgttt
      601 ttggatatct ggaaaattta agggtcaagg atattctaat ctattgctac aagagtgtgt
      661 tgatgatgca aaatcaaaag gtaaatatgg attagttgtg ctttcgtcta gtaaaaaaat
      721 gcctttttta tctgatgcta aatatctaaa atataagggc ttttgtttag cagatacatc
      781 agaaccaaat tatgagctat tatattttcc ctttactgat aatgctccta ttccatgttt
      841 taagaattgt gtaaaaaaaa gaaaattaaa tgagaacggt atagttttat attacagtaa
      901 tcagtgtcca catacagaaa aatatgctcc tattttgaaa aaaattgcaa attctagagg
      961 tattgatgta aatcttataa aattcgaaac agcaaaagaa gcacaaaatg ctcctgcacc
     1021 atttactaca tacagtcttt tttttaatgg tgaatttgta accaacgaga tactttctga
     1081 gaaaaagatt gagaaatttt tagataccaa cattaaaaaa atttaactaa aaatagatgt
     1141 aaatattgta ttttgagatt acattatttt ataatccaat atattttgga aatttcttaa
     1201 gttgatgaat gataatataa agattataac tcaatttaga tacaaaatgc tggaaattaa
     1261 ccatatagaa aatatttcca aaaatttaaa aattatattg acaaagttaa aaaaataaac
     1321 tatcatataa aacaataaga aaataaattg aaatcctgtg aaaaagagag tagcataaag
     1381 ctagttgata gagagctggg aatggtggaa gcctagtaac gaattttatt gtgaatagag
     1441 ctttggagga gattaccaga agtaatagta tggtaattgg tgaatctcgc cttaagagat
     1501 tagagtgggt aagattatct tatcaattag agtggtaacg cgggtgtata ttcgtctctt
     1561 ggtctttgac caagaggctt ttttaatttt aaatgtacat tttgtagata ctttttaatt
     1621 tgagttttaa tgattattgt aattttacca atgagagtgg taccgcggaa aatcgcctct
     1681 tagtatttat aatgctaagg ggttttttgt tttaattaga atattttaat ctacatatta
     1741 tcaaatgatt atcatatagt aaagtggaaa gatagaattt taatactatt tgaatataaa
     1801 aatggaggga acaaaatgaa taaaataaaa gttggtatta ttggcgcaac tggatatgtc
     1861 ggggcagagt tgattagatt attaatgaat catgataaag tcgaagtgac agcaataggt
     1921 tcaaattcgt atgttggaaa aaatatagtt gatatatatc caagtatagg atataaaaat
     1981 aacatgatat gtatagaaaa tgaaaaagtt atagatatgt gtgatgttgt gtttacagcc
     2041 cttccacatg gagttagtga aaagtttgta ataaaagcaa ttaaatcgaa aaagaaagtt
     2101 atagatttag gtgctgactt tagaataaaa gatgaagaag tatatagtaa atggtatgga
     2161 gtatctttta tagataaaat cttacataaa aaagctgtat atggattaag tgaaatatac
     2221 aaagaagata taaaagatgc agatataatt gcaaatcctg gatgttatcc aactagtatt
     2281 tctttaccat tgatgccatt actaagttct aaattgataa aaaataataa tataataatt
     2341 gattctaaat caggtcttac aggtgctggt agagaattat cgattagtag tcactttacg
     2401 gaggttaatg aaaatatcac tgcatataaa atagggaaac acagacatac tccagaaata
     2461 gaacaaaatt taagtgaatc ctgcaaagaa aaagtaagtg tagtttttac accaaactta
     2521 ataccagtaa ataggggaat cttatcaact atatattgta caaaagaaga taatatttct
     2581 ataaatgata tacatagaaa attaactgat tattatgaat ttaaagaatt tatagaagtt
     2641 ctcccattag ataaggtagc ttctttaaag aatgttaggt tctcaaataa atgtgtcata
     2701 tcattacatg aaaatggaga tacattgata atctgttcag ctatagataa tatgattaag
     2761 ggagcagcag gtcaagctat acaaaacatg aatataatat ttggtattga agaaaataca
     2821 ggtttaaaaa atattgcacc atcattttag taatgaaaaa atactaaact catataatta
     2881 ggaggataaa tatggagatt ataaagggtg gagttacagt ttcagaagga ttttttgctt
     2941 cagggattca ctgtggcttg agaaaaaata aagaaaaaag agatttagct ttggtttatt
     3001 cagatgtttt atgtgatgca gcagcagtag taactcaaaa taaagtaaag ggcaatcctg
     3061 tttatgttac acaggaacac attaaaaatg gaaaagcaca agctatcata gtaaatagtg
     3121 ctaatgctaa tacctttaat ggaaaagaag gacttataaa tgcttataaa atggctaaat
     3181 tcactagtga taaattaaat attaaagaaa atgatgtttt ggttgcatct acaggtgtca
     3241 taggaaagcc attaaatata aatttaatag aagaaaatat ggatgaatta gtgaataatc
     3301 taagtaaaca aggtcatatt ggagcaagag aagctattat gacaactgat attataaaaa
     3361 aagaaatagc agtagcaata atgtatggag ataaaaaaat aactattgga ggtatggcaa
     3421 aaggttctgg catgatacat cctaatatgg caacaacgtt aggatttata actacagatg
     3481 caaatattga tggagttttg ctaaaagaag ctttgaaaat tgctgttgat aaaagtttta
     3541 atagagttag tgtagatgga gatacaagta ctaatgatat ggtgatgatt ctagcaaatg
     3601 gaaaagcaaa aaatgataga ataaacaaaa aagatgaaca ttatcaagta tttttgagtg
     3661 cacttacata tgtctgtata gaacttgcta aacttgttgc aaaagatgga gaaggatgta
     3721 caaaactaat tgaatgttat ataaatggag cattaagtga agaacatgca gtaaaattag
     3781 caaaaacagt tatatcttct tcattagtaa agacagctgt atttggagct gacgcaaatt
     3841 ggggtagaat actgtgtgct ttaggttatg caggtgaaga aatagatatg gaaaaagttg
     3901 atattatttt tgagagtatg aaaggatata ttgaagtatg taaaaatgga aatggattag
     3961 attttaatga ggaaaaagca aaaaaaatcc ttgaagatga cgaaataagt atattagtag
     4021 atttaaatat gggcaatgcc agaggaaatg catggggatg tgatttaagc tatgattatg
     4081 taagaataaa tggaagttat agaaattagg aggatatact ttaatgatta atatagaaaa
     4141 agcaaataca cttatagaag ctttaccata tatagaaaaa catcagggaa agactattgt
     4201 agttaagtat ggtggaagtg caatgaaaaa agatggtcta aaagaatctg ttatggaaga
     4261 ccttgtatta atgagttatg ttggaatcaa tatagtttta gtacatggtg gaggggcaga
     4321 aatcaataag atgctagcaa aagtggatat agaaagtaaa tttgtaaatg gtcttagata
     4381 tacagatgaa gagaccatgg aaattgtaaa aatggtactt gctggaaagg ttaataaaga
     4441 cctagtaaat aaaatacata caaaaggtgg aaaagcagta gggctttgtg gaatggataa
     4501 taatatgatt ttatgtgacc catataaaaa ttatgaactt ggatttgtag gagaaatcaa
     4561 aaaagtaaat gttgagctta tagaatcttg tctaaagtca ggttatattt cagtaattgc
     4621 aactataggt gttggagatg atggagagac ttataatata aatggtgaca cagcagcttc
     4681 tgctatagct aaagagttaa atgcagataa attgatactt cttacagatg tccctggttt
     4741 gttaagagag ccagatgaag aaaaatcatt aataacagaa gttatattag aggatgttga
     4801 taaattattt gaagaaggta taattactgg aggtatgata cctaaaatag agggatgcgt
     4861 agatgcctta aataacggag taaatagggt acatattttg gatggaagag ttccacattc
     4921 tataataaca gagctattta ctgatagtgg aataggtaca ttaataagaa aagaaaatga
     4981 ataaaattat aagtttaaga ggggagaata agaatgaata acaacaatac aatttcaaaa
     5041 tggaatgaat attttataga tacatataac caacccaatt ttgtcataga ttatggtgaa
     5101 ggaagttgtt tttttgatac taatggcaat aaatacatag attttacaag tggatatgga
     5161 gtatcttctc ttgggtatag taatagtaat ttaaagaatg ctctaaaaga gcaagtagat
     5221 aaattattac atacttcaaa tttatatttt aatgagccag tgttgtattc aggagagaaa
     5281 ataattaatt caagtggaat ggctaaagta tatttttgta attctggaac agaagcaaat
     5341 gaaacagcat tcaaaatagc aagaaagtat agtagtgaca aatatggtaa tggtagagga
     5401 actatcataa gtttaaaaga ctcttttcat ggtagaacaa tgatgtcttt aatggcaact
     5461 ggtatggaca aataccataa gtatttttat cctcttcccg aaggttttaa atatatagaa
     5521 agaaataata ttgaagattt aaaaaataat cttgattcca cagtttgtgc aataattctt
     5581 gaagcgattc aaggcgaagg tggagttaat gttcttgaaa aagattatgt tttggaaata
     5641 gtaaaaatat gtcaagaaaa ggatattgta gtgatttttg atgaagtcca atgcggtata
     5701 ggaagaactg gtaaactttt tggctatgaa tattttgatg taaaaccaga tatagttacc
     5761 gtagcaaaag ggttgggagc aggaatacca gtaggaggag tattggtaaa taaaaaactt
     5821 tcaaaagtac taggaaaagg agaccaaggt acaacttttg gaggtaattt attagctatg
     5881 gtggcagctt cagttgtatt agatgagata tctaaagatg gtttttataa tgaagtatta
     5941 gaaaaaggaa attatataag aaaatctatt gaaagtttta ataataaagt tgtattaaag
     6001 acaaaaggta ttggtctaat gataggaatt gaaactaata ttgaatctag tattatagaa
     6061 gagaaagcta gaaaaaaagg attgcttatt ttgactgctg gaaaaaatgt attgagattt
     6121 ttaccaccat taacaatcag ttataaggaa atagatgagg ctcttgaaat cctaaaagat
     6181 atattactag agattaacta aatattcaaa aaatagttaa ttaaagataa aaattagaag
     6241 taaataaaaa agtaaagtgc taacaagttt aattgaattt aagaagttta taagaagggg
     6301 cgaattaata tgaataatat aagtttaaaa ggtaagagtt ttttgacatt aaaggatttt
     6361 acaaaagaag agattagata tttattaact ttatcaagtt atttaaaaac taagaaaaaa
     6421 gttggcataa aaggcgattt gctacaagga aaaaatatag ctttattatt tgagaaaaca
     6481 tcaacaagaa ctagatgttc atttgaagta gcagctcatg atgaaggagc atatgtaact
     6541 tatctaggtc caaatgattg tcatatgggt aaaaaagagt ctgtatcaga tacagcaaaa
     6601 gttctaggaa gattctatga tggaatagaa tttagaggct ttaaacaaga aacagttgaa
     6661 agtctagcaa aatattctgg tgtacctgtt tggaatggac ttacagatga atatcatcca
     6721 acacaaatac tggcagattt tttaacagta attgaaaatg tagataaaga tttgaataaa
     6781 gttaaatttg tatatgttgg tgatgctaga aataatatgg gaaactcact gatgatagga
     6841 tgtgctaaaa tgggaataca tttcgtagca ctagcaccta aggaactttg gccaaatgaa
     6901 gaattggtta aggaaatgaa ggaaatagcc aagaaatcca aaggagatat aatgttgact
     6961 gaaaatattg atgatgttaa aggtgcagat gttatatata cagatgtatg ggtttctatg
     7021 ggagaagagg agcaatatga agcaagaata aatcaactta aagattatca agtaaatatg
     7081 gatatgataa agaaaactga aaatgaaaat gttatatttt tacactgtct tccagcattt
     7141 catgatttag aaactaaggt tgctcagaag atttatgaaa agttcggatt atcagaatta
     7201 gaggttacaa acgaggtatt taattcaaaa tattcaaagg tatttgaaga agctgaaaat
     7261 agaatgcata caataaaagc tgttatggtt tctactatga ctgatgtaaa tatgaacaat
     7321 aatttaatat agatatgttt taatatagac tttactttaa tgaaaattta taaaatattt
     7381 taaatatatt attcaaaaac tctcattata tgttaaaata acttatgtat caacaaatta
     7441 agagggagga tttgagatat gaaaaaaatc atattagctt taacaacttt tttattagca
     7501 atatctttaa cagcatgttc aagtgcaaaa ccagaagata caattgatag tttttttaac
     7561 agtgcgaaaa agtttgactt tgaaggcatg aataaggtaa tggaaaataa tgacgaaaaa
     7621 tataaggata tattaaagga attagataca aaagacccaa atgcccaata tgttttagat
     7681 tatctaaaac agaatgcatc taaaatcacg tatacaataa aagacagcga ggttaaaggt
     7741 gataaggcta caatcaaagt agaatgtaaa tttatagatt caacaccatt acttcaagaa
     7801 atagtagctg aggcatttac aaagatgatt ggtatgagtt tttcaggaca agacttaact
     7861 gatgagaaaa caactgaaat gttagtatca attatgaaag aaaaacaaaa aagcgttaaa
     7921 gagacttatg ttacaaaaac tgtagagttt gaatgttcta aaaaggataa taaatggata
     7981 ataagttcag taaatgatgc agtagctgat gtattgttat ctaatcttgt tacagcagga
     8041 caagaatttt ctaactctat gaatacactt gaataatatt gtttaggtgt agtaaagtaa
     8101 taaatatatg attattttta taaataaaca tataaatatt tatgaaggat accatttttg
     8161 gtatccttt
//
LOCUS       DAEPLF010000155         8076 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEPLF010000155 DAEPLF010000000
VERSION     DAEPLF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.155"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            131..337
                     /gene="yaaA"
                     /locus_tag="KR314_002753"
     CDS             131..337
                     /gene="yaaA"
                     /locus_tag="KR314_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBH0329123.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVKIEGTKIKVL"
     gene            355..1470
                     /gene="recF"
                     /locus_tag="KR314_002754"
     CDS             355..1470
                     /gene="recF"
                     /locus_tag="KR314_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBH0329124.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            1471..3372
                     /gene="gyrB"
                     /locus_tag="KR314_002755"
     CDS             1471..3372
                     /gene="gyrB"
                     /locus_tag="KR314_002755"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBH0329125.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            3392..5818
                     /gene="gyrA"
                     /locus_tag="KR314_002756"
     CDS             3392..5818
                     /gene="gyrA"
                     /locus_tag="KR314_002756"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBH0329126.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDIAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKIGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKNEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            5887..6183
                     /locus_tag="KR314_002757"
     CDS             5887..6183
                     /locus_tag="KR314_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBH0329127.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            6183..6443
                     /locus_tag="KR314_002758"
     CDS             6183..6443
                     /locus_tag="KR314_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBH0329128.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            6455..6772
                     /locus_tag="KR314_002759"
     CDS             6455..6772
                     /locus_tag="KR314_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBH0329129.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVKKIFTITGLDKIFK
                     VEG"
     gene            6797..7183
                     /locus_tag="KR314_002760"
     CDS             6797..7183
                     /locus_tag="KR314_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0329130.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            7183..7956
                     /locus_tag="KR314_002761"
     CDS             7183..7956
                     /locus_tag="KR314_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBH0329131.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
BASE COUNT         3315 a          784 c         1572 g         2405 t
ORIGIN      
        1 tgaaggagtt gtcttttgta gaaaattttt ctaatcttag acaactcctt ttataagcat
       61 atttatggta taatatactt ttagtgtatc aataggaatt tgtttaaaat agtgacaagg
      121 aggaactatt atgactgaaa taactataga atcagaatat ataaagttgg atcaatttct
      181 taaattagca gagattgcat caacaggagg tcatgcaaaa tttttaattc aagaaggttt
      241 agtgacagta aatgatgaaa tagaattaag aagaggaaaa aagataaaat caggagatat
      301 agttaaaata gaaggaacta aaataaaagt attgtaaaaa aaggagaact ttttgtgaaa
      361 cttaaaagtt tgcaacttgt taattttaga aattataaaa agttgcatct ggaatttaat
      421 gggaaagtaa atctacttgt tggtaaaaat ggtcaaggaa agacaaatat agtagaatct
      481 atttatatgc tttcttttgg aaaatcattt aggactaaca aagataaaga aatggttaga
      541 tttaatagtg aaaacttata cattggagga agtttttcaa aatataataa atatagtcta
      601 atagagctaa ttattggaaa agataaaaag gggataagaa taaataaagt tcctttacaa
      661 aagatacaag aactacttgg caacttaaat gttgttatat tttctcctga agatttaagg
      721 ttggttaaag agggtcctaa agaaagaagg gcatttatag acaaagagat tagtcagatt
      781 atacctaaat attataaata tcttaccaat tataataaaa cactttctca aaggagtagg
      841 gtgcttaaaa atatacatgt tgatgaagct ttattagatg tatatgatga tacgttagca
      901 aagtatggaa gttatattta tattttgcgt agagacttta taaaaaagat agctaatata
      961 tctgaaaata tgcatatgaa tttgactaat ggagttgaaa gattatcaat tcgatataaa
     1021 aatcaaataa atataactga tgaagatact atagatacag tatataataa atttttagcc
     1081 aagttgtcat cgaatagacc taatgatata gaatctaaga ctaccagata tggtatacac
     1141 aaagatgact taaatatatt cataaatgat ttagatgcaa gactttttgg ttcacaaggt
     1201 caacaaagaa ctgcttcaat atcattaaag ttatcagaga tagaattaat aaaaaatgaa
     1261 gttgaagaat atccagtatt aattttagat gatgttttta gtgagttaga tgaagcaaga
     1321 caaaaacttt tagtaaataa tttaagcaat gttcaaatgt ttataacaag tgctgaagtt
     1381 tcacataaaa aaatatttga tgaaaaaaat gttacaatat ttaatattga aaatggcgat
     1441 gttattagca tagagaatgg aggaaattaa atgaaacaag aatacggtgc aagtcagata
     1501 caagtattag agggattaga agccgttaga aaaagaccag gtatgtatat tggtagtaca
     1561 agccctagag gtttacatca cttagtttat gaagttgtag ataatagtat agatgaggct
     1621 ttacaaggat attgttcaga catatatgta tctattaatg aagatggcag tgttttagta
     1681 aaagataacg gaagaggtat acctgttgaa atacatccta aaactggtaa atcaacttta
     1741 gaaacagtat taactaatct tcatgcagga ggaaagtttg gaggcggagg atataaagta
     1801 tctggaggtc ttcatggagt tggtgtttct gtagttaatg cattgtcaaa atggatggta
     1861 gcagaggttt atttaaacgg aaagatatat aagcagacat atgagaaagg attaccaaca
     1921 tctaagcttg aagtagttgg agaatctcaa gataaaggaa caatgattca atttatgcca
     1981 gatgagacaa tatttgatga aatagagttt aaatatgaaa cattggagta taggcttaga
     2041 gaactttctt tcttgaataa aggaataaaa atagtatttg aagataaaag agaaggacaa
     2101 gaaaaaagaa aagaatttca ctatacaggt ggtttggtag aatatattaa gtatttaaat
     2161 aaatctagaa ctggtataca tgatgacata gtttatatag ataaaaaggt tgatgattgt
     2221 tttgttgaac tagctatgca atatacagat ggttatacag agaacatata ttcttttgca
     2281 aacaatataa atacacacga aggtggttct catttaagtg gatttaaagc agctcttact
     2341 aaaactgtaa atgattatgc taagagaaat aaatttttaa aggaaaatga tgttaaccta
     2401 cttggtgaag acataagaga aggtcttaca gcagtagtat cagttaaatt accagaacct
     2461 caatttgaag gtcaaactaa aacaaagcta ggtaatagct ttatgagagg tatagttgat
     2521 agtgtaacag ttgatgaact ggggtctttc ctagaagaaa acccatcaac agcaagaatc
     2581 atagttgata aagctttaag agcacaaaga gctagagaag ctgcaaaaaa agcaagagaa
     2641 ttaacaagga gaaaaagtgt attagaaagt acatctttac ctggaaaact tgcagattgt
     2701 gcagaaaaag atccatctaa aagtgaaata ttcttagtcg aaggggattc agcgggaggt
     2761 tcagctaaac aaggtagaga tagaaatagt caagctatac ttccattaag agggaaaata
     2821 cttaatgttg agaaatcaag actagatagg atactatctt cagatgaaat aaaaaatatg
     2881 ataacagctt atggttgtgg tattggagaa gattttgata tagataaggc taggtatcat
     2941 aaaattataa ttatgaccga tgctgatgta gatggagccc atataagaac tttattatta
     3001 acattcttct ttagatatat gagacctctt atagatgagg ggtatgttta tgcagcacag
     3061 ccacctttat ataaagtgac aaagcaaaag aaggaacatt atgtttactc agataaagag
     3121 ttaaatatct tattagatga aattggaaga aatggtgttg aacttcaaag gtacaaaggt
     3181 cttggagaga tgaatgcaga gcaattatgg gaaacaacta tgaatcctga gacaagaact
     3241 ttattacaag ttactgtaga agatgctgca attgcagatg aagtattttc aatgcttatg
     3301 ggtgataagg ttgctccaag aaaagaattt atagaagaaa atgcaagatt tgttagaaat
     3361 ttagatatat agagagaggg gattagtata catggaagaa aataacaaaa tactccctat
     3421 tgaaatagcg gaagaaatga aaaaatcgta tattgattat tcaatgagtg ttatagctgg
     3481 acgtgctctt cctgatgtta gagatggttt aaagccagtt catagaagaa tattatattc
     3541 aatgagtgag ttaaatttaa ctccagataa accatacagg aagtcagctc gtattgttgg
     3601 ggacgtttta ggtaagtacc atcctcatgg agatattgct gtttattatg ctatggtaag
     3661 aatggcacaa gatttttcaa ctagagcact tttagtagat ggtcatggta actttggttc
     3721 tgttgatggg gattcaccag ctgctatgcg ttatacagaa gctaaaatga gtaaattatc
     3781 attagaacta ctgagagata ttgaaaagga aactgtagac tttaaaccaa actttgatga
     3841 gtcgttaaaa gagccttcag tattgccagc tagatatcct aatttattag taaatggctc
     3901 aaatggtata gctgttggta tggcaacttc aatacctcca cataatttag cagaagtaat
     3961 tgatgcaact gtatatttga tagataatcc agagtgtagt gtagatgatt taataaaatt
     4021 tgttcaagga ccagatttcc ctacagctgc aattataatg ggaaaagaaa gtatagcaga
     4081 agcatacaga actggaagag gaaaagttaa agttaggtct agagctttta tagaagaact
     4141 accaaaagga aaacagcaaa taatagttac agaaatacct tatcaagtaa ataaggctaa
     4201 actggttgaa agaatagcag agttagttaa agaaaagaga atagaaggta tatcagacct
     4261 aagagacgaa agtaatagaa atggtatgag aattgttata gaattaaaga gggatgctaa
     4321 tgctaatata gtattaaata atttgtataa acattctcaa atggaagata cttttagtat
     4381 aataatgctt gcacttgtag atggtcagcc aagagtttta aatcttaaac aaatattata
     4441 tcattatatt aaacatcaag aagatgttgt tacaagaaga actaaatttg aactaaataa
     4501 agctgaagca agagcacata ttttagaagg attaaagatt gctttagata atatagatgc
     4561 tgttataagc ttgataagag cttcaaagac tgggcaagaa gctaagatag gtttaataga
     4621 aaaattcaaa ttaactgaaa tccaagcaca agctatatta gatatgagac ttcaaagact
     4681 tacaggttta gaaagagata agatagaagc tgaatatgaa gatttaatca agaagataaa
     4741 tagattaaaa gaaattttag ctgatgaaag attactttta aatgtaataa agaatgaaat
     4801 tacaataata aaagaaaatt actctgatga gagaagaaca gaaataagac atgctgaagg
     4861 cgaaatagat atgagagatc ttataagtga tgaagaaata gcaataactc ttactcactt
     4921 tggatatata aaaaggcttc catctgatac ttataagagt caaaaaaggg gtggaagagg
     4981 tatttcagca cttacaacta gagaagaaga ttttgtaagg cacttggtaa ctacaactac
     5041 tcatagtaga ctattattct ttacaaataa aggtagagta tttaaattaa atgcatatga
     5101 aataccagaa ggtaagagac aggctaaagg tactgctata gtgaatttac ttcaattgtc
     5161 agcagatgag aaaattgcta ctctaatacc tattgatggt aatgatgaaa atgaatattt
     5221 attacttgca acgaaaaaag gtattgttaa aaagactaag agagaagagt tcaaaaatat
     5281 aaataaatct ggtcttattg ctataggttt aagagatgat gatgagctta ttggagtaga
     5341 acttacagat ggaaaacaag aagtactttt agtaactaaa gaaggtatgt ctataagatt
     5401 tgatgaaaat gatataagat atatgggtag aacagcaatg ggtgtaaaag gtataacttt
     5461 aagtaaagaa gattttgttg tgtctatgaa cctttgcagt aaaggtacag atgtgttagt
     5521 tgtaagtaag aatggttttg gaaagagaac aaatatagaa gagtatagaa gtcaaataag
     5581 agctggtaaa ggaattaaaa cttataatat atctgaaaaa actggtacaa ttgtaggtgc
     5641 agatatggtc aacgaagatg atgagataat gattataaat tctgatggag ttcttattag
     5701 aataagagtc aatgaaatat cactgtttgg aagagttaca agtggtgtta aattaatgaa
     5761 gacgaatgat gaagtcaatg tagtttcgat tgccaaaata aatattgaag aagaatagga
     5821 taaatatcct attctttttg tataatgtag ttagataaaa atttaaaatt gaaaggagta
     5881 attgttatgt gcaataaaag aagttgtaaa aaaggtggat ttttattatt aggagctatt
     5941 ttagggttta tatttggtat gttttttgca cctaaaaaag gttctgaact aagaaaagaa
     6001 acaaaagaga agtttaatga tgtcaaagaa aatccaaaag aagttctaca tgaaacattt
     6061 aatgatgtta aagagagaat aataaattta gtggacgatg ataataatga agaagatata
     6121 aagatttcag aagaagatat agtaataagt aaaagctttg atgatgaggg agatgttaac
     6181 taatgaatgc atgggggtgg caaattggag cagtgttagt aggaagctct gctcttatag
     6241 tagcaattta tttagctaaa actctaaaca gcataaataa ggttgttgaa aaggcatata
     6301 agattgtaga ttataatgaa agacatatac aagatattgt tgaaaatgca tcatcaatat
     6361 caaagggaat agatgatata gtttatgtaa ttaataaaat attgagtata ggaaatatat
     6421 ttaaaattat taaaaggaaa taggaggttt ttacatgtct atgaatattg attcaaattt
     6481 agattcacaa aataaatttt ggaatgtttg tcttgatgga gaacttgatg tttctacagc
     6541 agataaacta aaggaacatt tacatgcttt aatagaaaaa aatatgttag atgtaaagat
     6601 taatcttaaa gatttagatt atatagactc aacaggctta ggagctatga taggtgtatt
     6661 aaaaaaatta aaaataaatg aaaaagagat atacatagta aatcctaaga gtaacgtaaa
     6721 aaagattttt actataacag gtctagataa aatatttaaa gtggagggat aaactttggc
     6781 ttgtgagact ataaagatgg aaattactac aaatccagat tatgttagta taataagatt
     6841 aacagcatct ggaattgcca ataagatggg ttttccaata gatgatatag aagatataaa
     6901 ggtagcagta tctgaagcat gtacaaatgc aataaaacac agtaaagatg atgtgtttaa
     6961 tatagtattt aatattttag ataatgcaat taatatagaa attcaagata atggaaaagg
     7021 atatgatgtt agctcaataa gtacaccaga tttagagaat ccaaaagaga gtggtcttgg
     7081 attgtttata attaaaactt taatggatga tgtagatatt gaatcagaac ataatcaagg
     7141 gacaaaaata aaaatgacta aatatttagg agttgatatt taatgaaaaa tgtagctaat
     7201 gctacacatt acctaaatat ggatacaaaa gagctattta atttatataa taaaaataaa
     7261 aatgtagaca ttagaaatat tcttattgaa agacatctgt atttagcaag attgttagct
     7321 aagaagtata taaacaaagg tgttgatttt gaagatatat atcaagttgc ttcattggca
     7381 ttaatttatg ctattgatag atatgatgtt gagaaaggat ttgagttctc cagctttgcg
     7441 acaccaacta tagtaggaga aataaaaaag tattttagag ataaggtatg gacacttaga
     7501 gtcccaagaa gaatacagga attaagcaaa aagataagtg atgcgaagat taaattagaa
     7561 caagaaaata agaagcatcc aaaggtaaaa gatatagctg attatatagg cgttagtgaa
     7621 gaagatgttt tagaggctat ggaggcttct tatggatatc aaccaatgtc tttggattca
     7681 tcaagtaatg atgattcaga ggataaagat ataactctta ttgataagat tggaaaagaa
     7741 gaaggtaact ttggaagtat agagtatgaa gattttatta ataagtttat tgaaacttta
     7801 aatgaattag aagttaaaat ttttaaagat agatttttct ttgataaaac acaatctagt
     7861 atagctaaag aactagggat atcacagatg actgtttcta gactagagaa aaaggtagta
     7921 gagaagctga aaaaagaata tgaaaaaaat ttataaaaaa atataaaaaa agtattgacc
     7981 tactgtttta aagatggtat agtattactt gtccttgaaa aaaggaacaa gaaataaaca
     8041 cgaacattga aaattaaaca gtaggttaat ttatat
//
LOCUS       DAEPLF010000156         8062 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEPLF010000156 DAEPLF010000000
VERSION     DAEPLF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.156"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            285..992
                     /locus_tag="KR314_002762"
     CDS             285..992
                     /locus_tag="KR314_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0329132.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            986..2296
                     /locus_tag="KR314_002763"
     CDS             986..2296
                     /locus_tag="KR314_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0329133.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMAQE
                     NYLKDTTESEETGYLVEYNEYFKEGTFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEKLEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            2413..3096
                     /locus_tag="KR314_002764"
     CDS             2413..3096
                     /locus_tag="KR314_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329134.1"
                     /translation="MKILYTENLSKHYGKGENLVRALDNVNLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            3089..5476
                     /locus_tag="KR314_002765"
     CDS             3089..5476
                     /locus_tag="KR314_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0329135.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFELIKGKLPTKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKNDK
                     KIEKEFVISGILKTSEISEAGKYYYAIISELYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGGFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKNNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNLIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKAVISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            5755..6897
                     /locus_tag="KR314_002766"
     CDS             5755..6897
                     /locus_tag="KR314_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBH0329136.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIVGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLDRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALKLTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNENLLNLFNKYYF"
     gene            6916..7440
                     /locus_tag="KR314_002767"
     CDS             6916..7440
                     /locus_tag="KR314_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0329137.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYITEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            7540..>8062
                     /locus_tag="KR314_002768"
     CDS             7540..>8062
                     /locus_tag="KR314_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0329138.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSELFAKKLEESLNPTQSVEKIVLYLEEMLLSILEEDLISNKSV
                     KLLNSFIYDNRYSN"
BASE COUNT         3295 a          746 c         1414 g         2607 t
ORIGIN      
        1 gtagatagta atttaatttt agataatagt aaacagcaaa ggaaattagt tatataaata
       61 ctaatttcct ttgttttatg ctttaacatg attattttta aataaaattg tattttatga
      121 aaaataatta cactaaatgg catagtatta tggggatttt tagtttttat tcaagtaaat
      181 gaaatcatat taacaacttc tgatatattt acaaattgat aaaaaaatga ttttattagt
      241 atgatataat ttagctagta tatatgatga tggaggatat agggatgaat aatatattac
      301 ttttggaaga tgacaaatct ttaaatagag gaattagttt taaattaaaa aaagaaggat
      361 acaatgtttt tagtgcattt ggtatagaag aagcaaaaaa tattttcgca aaagaagaga
      421 tatgcttaat tatttccgat ataggtctac ctgatggtag tggttttgac ttttgtgagg
      481 agattagaaa gaaaagtgat gtttacataa taatgttgac agctcttgat gaagaggtgg
      541 atattgtcac aggatatgat ttaggtgcag atgattatat cacaaagcct tttagcttga
      601 tggtattaat atcaaaagtg aatgcgctta tgaaaagagt aaatacagta aaaaattata
      661 ctctattagt ttgtgatgat ttattctttt attatataga aaataaatta attataagaa
      721 cagatgataa ggaagaagag ataattttaa gtaagacaga gacaaaacta ttaaaatatt
      781 tgatggaaaa ttctatgcag acattgacta aagaacaact gttagaatct ttatgggata
      841 gtagtggtaa ttttgtagat gataatacga ttgcagtaaa tataagaaga cttagacaaa
      901 aggttgaaaa aaatccatct gaacctaaat atataaaaac agtaagaggg gttggatata
      961 tgtggggaga aaggagtata aaaaaatgct aaaaaatatg aacctcaaag atggtttaag
     1021 tgtgaaagat gattatataa tgagaagagt tattgtatat tctgttttat ctgtactaat
     1081 agtaaccctt gtatttatac tttttgaata tagcagatta cataaattgt atgaacatca
     1141 aataaaaaat actcaaaata ttgctggaat attgattgaa aaatatccta atgatgaaat
     1201 agatatagta aattctattt ataattccga ttatagtaag gtgtctactg gagaaaaggc
     1261 atttaagaaa tttggctatg gattggaaaa taaaatgtca aatgataaaa attttagttt
     1321 atacttaaaa tcttttttta aggaatcatt ttttattttt ttaatgatgt ttatagtagt
     1381 tataggtata atatattatt ttataagata cataaataaa agattgagta aaatttattt
     1441 tatagtagat aagatggcac aagaaaacta cttgaaggac actactgagt cagaagaaac
     1501 tggttatcta gtagaatata atgagtattt taaagaaggt acatttagta agataaataa
     1561 ctgtctatat gagcttaata gaagtttaaa gataaagttt attaaattag aaaaagaaaa
     1621 agaaagtgta aaatcattag taacagatat ttctcatcaa ttaaaaactc ctttagcatc
     1681 cttaaaatta tacaatacat tacttataga agaaaaatta gaagaagatg aagccaacga
     1741 gttcttatta accaataagg cttctataaa taaattagaa aatttaataa attctttagt
     1801 gaacatatca aggttagaaa ttagcatgat aaacataaaa aaagaagata atgatataaa
     1861 aaatactatt ctaaatgcag taaaaagtgt tacccctaag gctaagagca aaagtataac
     1921 attaaattta aatgaattta acagtagaat tataccacat gataaaaagt ggactgagga
     1981 gagtattttt aatgtattag ataatgctgt aaagtacaca caggtaaatg gagaaataaa
     2041 tatatctgta gaagaaactg ctaatttctt taaaattata atagaggata atggcatagg
     2101 gattgaaaaa aatgagttta ataatatatt taaacgtttt tatagaggtt ctgctgatga
     2161 aattgaatct gtagaaggta gtggggtagg attgtattta agtagaaaga ttttagaaga
     2221 acaaggtgga aatattatag tttcttcaaa agtagggata gggagtaagt tttctttgtt
     2281 tcttacaact atgtaatatt aaatgaaagg aaaatgtaag cctctacatt tattatataa
     2341 gtataaacag ctccagaaat aaaaaattaa taaaatatgg ggtaatataa cttagaatga
     2401 ggggataatt ttatgaaaat actatatact gaaaatttat caaagcatta tggaaaagga
     2461 gaaaacttag taagagcttt ggacaatgtt aacttagaaa taaatgaagg tgaatttgtt
     2521 gcaataatag gaaaatcagg ttctggaaaa agtacacttt tacatatgat tggaggtctt
     2581 gatataccta catctgggaa ggtgtatata gataataaaa atatatttac attaaaagaa
     2641 gaagaactag ctgtgtttag aagaagaaaa atagggttta tatttcaaag ctataattta
     2701 attccatctc ttaatgtatg ggaaaatgta gtgttgccaa tcggtctaga tggtagagaa
     2761 gtagatgaat cttttataaa agaacttttg aaatcattag gacttgaaaa taaacatgac
     2821 gttttaccaa atacattatc aggtggtcaa caacaaaggg tggcaatagc aagagcatta
     2881 gctacaagac cagctataat acttgctgat gaaccaactg gaaacctaga ttctaaaaca
     2941 tctgatgagg ttatgagtat attaaaatct atgtctaaga agtacagtca aactctagtt
     3001 atgataactc atgatgacag tatagcccaa atggctgata gagttatatt catagaagat
     3061 ggcagggtgt caaaggttgg tgataaaaat gattaatact actaaaatag caaaaagtaa
     3121 tttaaaacaa aataaatcaa aaagtattct aataataatc actataatac tttctacttt
     3181 aatgctttct tctattggaa tttatattgt tgatgcagga gcatatcaaa aagaaaatac
     3241 tataaagtat tcaggtaatt atcaaggtat attagcaaat gtagatgaaa aacaggctga
     3301 tatattgagt aatcatgcag atgttgaatt aactggtgaa atgaatggag taggtgttga
     3361 aaaactggaa gatgattcaa atatttctct agcatacatg aatgaagatg ctttgaaatt
     3421 aaatagtttt gaacttatca aaggaaaatt gccaacaaaa gagaatgaaa ttgtgttaga
     3481 tagtggagct ttaaaggcac ttggttataa aaataaagac ttaggtgaaa agataaaaat
     3541 atcttataat gactataaaa atgataaaaa aattgaaaaa gagttcgtta taagtggaat
     3601 cttaaaaact agcgaaatat ctgaagcagg taaatattat tatgctatta tatcagaatt
     3661 atatatgaga aatactagag atatgtcaca agaagatttt aatatttatg taaaatttaa
     3721 tgataaaagt aatttatcaa tagagcaagc aaaagaaaaa ttagataaaa ttgctaatga
     3781 tataggactt gatactatta atactgctgt aaatgaaaat tatataaatg ctttaaaacc
     3841 agatatggaa acgattatgg gtggaggttt tgtaggatta gtaattgtat tatctagtat
     3901 tttagtaata tacaatatat tttatatttc aatagttaca aaagttcaag aatttggaaa
     3961 acttagagca attggtgcaa ctaagaaaca aataaaaaat atagtgttta aggaagggtt
     4021 tatattagcc ggaatagcaa taccaatagg aataatttta ggctatgtac ttgcaaatat
     4081 tataataaaa tcatttatgg atatagatgt aaaatcatca cgattaccag ttatattatc
     4141 ggttgtggta ataagcttta tatctgttgt tttatcactt ttaaaaccaa tgaaagtagc
     4201 atcaaaagta tccattgttg acgcagtaag atatacagga aataaaataa gtaataagag
     4261 taagagaaaa ggatataaaa atataaatct aaagagatta tcacatgcca atttagaaag
     4321 aaataaaaaa cgtacttaca tgacattggc atcacttata cttagtggaa ctatatttat
     4381 aacagtatca actgctttag aaagttttga tgctgagaaa atggcaagag aacactttcc
     4441 ttatgatata gaagttagac tatctggtta tgaaatgaac agtgataaaa atcctaaaaa
     4501 taatttgaat attttacaaa tggataatcc tctaagtaaa gattttttta atcaaataaa
     4561 gaacatggaa ggaattaaaa gaatagaatc tgcaagaagt gtaaaaattg gtatggagga
     4621 ctatgatgtt gaatttaagt atgacctttt acaaagtata aatgaaaatg atgtaaaatc
     4681 attaaacaag aatctaatag atggaaaaat aaatcttgaa agactgcaaa ctggagatga
     4741 aatagtaatt actcatgttg atacagcaaa agaaatggga gtaaaagcag gagataaaat
     4801 aagattaact ttatatgatg gagataaaaa gataaaaaaa gaatttaaag ttcaagcaat
     4861 agctatgggg gttccaagtt ttggtatagg gaaggatttt attgatagaa ctttaaaata
     4921 cgattcaact agtagcttgg gaatatatac agaagaagga aagtatcaag aaatcaaaga
     4981 tagtatcaag aagattgcta aaaataatgg atttttagag actgatttta tagatagtag
     5041 aattgagtca aataaggctg ttatttcatt tataaagata atgggataca cgctaacagg
     5101 cataataggt gtgattggat ttatgaatct tgtcaacaca atgataacta gcatagttac
     5161 tagaaaaaaa gaacttggta tgcttcaagc tattggtctt actaataaac aattagtgaa
     5221 aatgcttaat tcagaggcaa tgtattacac ttctggaatg atgataggaa gtattttatt
     5281 tggaggtata ttagggtata ttgctgttat atttctaaaa aagactggat tatcttatgc
     5341 aacatattct ttgccaatag taccaatctt gttgatgatt gtgtgtattc ttatagctca
     5401 atttataaca acttatttga taggacgaag ttttaataaa gaatctctta ttgatagagt
     5461 aagatacagt gaataaaatg caaatatttg tggttagttt aactagcaaa aagcatataa
     5521 aaaatatagt taaagagatt cataattaaa taggttaaag catagtacag agcaaacaat
     5581 ttaaacggat gtttgctctg tattttatta tgtaatcaat aatatatcta atatactgat
     5641 attattcatg ttagaaaatg tatcggtaca attctaaaaa agtataaaat tgtaccatat
     5701 aaaatgaaaa ttttgtataa taatcgtata gaaaataaat aggagctgat atatatgaat
     5761 aagaagatta attatgcacc aggaccaact gaaactaggg aaaatgtaag attagcaaga
     5821 gcagaaaaaa caacaaatcc agatatagat attgattttg ttgaatttta taaaaagaca
     5881 tgcgagaagt ttggcaatat agttggaaca aaaaacgatg tttacatatt gagtggtgaa
     5941 ggaatattag ggcttgaagc agcatgtgca tcacttaccg aaaaaggaga tagagtactt
     6001 gtaattgaca atggtatata tggagaaggt tttaaagatt ttgtaacaat gtatggtggg
     6061 gagtatgtgc ttttttcaag tgaatatact aaaagtatag atattgatga attgaaagca
     6121 tttttagatg aggatagtaa ttttaaatat gcaacagtag tgcattgtga tactccaact
     6181 ggcgtattaa atgatgtaag caaaatatgt ccattactta aagaatatgg aatattaact
     6241 gtagttgatt ctgtagcagg tatggtagga gaaagattaa gtgtggatga atctaaaata
     6301 gatataatat taggtggttc acaaaaagct atttctgcac cagcaggact tactatagtt
     6361 ggaataagtc aagatgcaaa aaattgtata aaaaatagaa aaactgatgt aataggtttt
     6421 tattgtaatt taaatatatg ggaaggctat tatgaaaaaa aatacttccc ttatacaatg
     6481 ccaataagtg acataatggg gcttgataaa gcacttgata atatattaga agaaggaata
     6541 gaaaatgtat tagatagaca tgaaaagata gcttatagtg ttagaaaatc tattgaggaa
     6601 tatggtctag aactattttt agaagaagga tattctaata cagtcacagc tataaaaatt
     6661 cctgaaagta taggagcttt aaaacttact gattatatgc ttaaaaatta taatacactt
     6721 gttgcaacat cactaaatca atatatggat actattttaa gaataggtca tatgggagag
     6781 aatgcaaatc taaataaaat agagcatgta ctaaatgtct tggataaaag tttaagtgca
     6841 ttaggattca aaggaaatga aaatttgttg aatttattta ataagtatta tttttaatag
     6901 atagggggat atagtatgga agttacagct aagcaaaaat ttacaacgaa agacatggtg
     6961 gaaacctcac tattaatagc attagtattt attgcaacta aatttataaa tataagatta
     7021 ccaatatcta taaatggtgg gcttgtgcat ttaggaacag caatgttgtt catatcagca
     7081 atagtatttg gaagtaaaaa aggtgcttta tcaggagcta ttggtatgtc actttttgat
     7141 ttaatatctg ggtggacttt atgggcaccg tttaccttta tagttagagg agttatgggt
     7201 tatttacttg gaaagattgc atgggcaaat ggtaataatg ggaataattt tttaatcaat
     7261 gtaataggaa tatgtgtctc atcaatttgg atgttatttg gatattatat aacagaagtt
     7321 atactttatg gtaattttat agtacctctt acttcaatac caggtaactt aatgcaagta
     7381 ttgataggtc ttataatagc gcttcctatt tctaaggtat tgaaaaaatg tataaaataa
     7441 atataaagtg aactaaaatt tatgatagca taaattatac tgtataatat taagatggag
     7501 tacataatta aaaaatttta gtatagaggt tagttcacaa tgttatcagt tacaagatat
     7561 tcaatcaaaa atgttcgttt aaagccgtgg gtaaagttta tttggtattt tgaaacaaaa
     7621 tcaaatatac tgttaaataa taagttactt cctacagaca gtatagatat tattcttaat
     7681 ctttctgatg ttatggaata taaaattgaa aatcaagact atactgctag taatatgcat
     7741 tttaatggaa taagggataa acatggattt attattcaac atggaaatat acgtgttata
     7801 gggatttctt tttatccatt tggtctctat ccatttttaa agataccaat ttcagagttt
     7861 aacagacaaa ttgttgactt agaagcagta tcagaactat ttgccaaaaa attggaagag
     7921 tcattaaatc caacacaatc agtagaaaaa attgttttat atttagaaga aatgttatta
     7981 tccatattag aagaagattt aatttcaaat aaatctgtaa agcttctaaa ttcatttata
     8041 tatgataata gatatagcaa ta
//
LOCUS       DAEPLF010000157         8052 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEPLF010000157 DAEPLF010000000
VERSION     DAEPLF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.157"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            542..904
                     /locus_tag="KR314_002769"
     CDS             542..904
                     /locus_tag="KR314_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBH0329139.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVNNVLK"
     gene            970..1587
                     /locus_tag="KR314_002770"
     CDS             970..1587
                     /locus_tag="KR314_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBH0329140.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            1584..2069
                     /locus_tag="KR314_002771"
     CDS             1584..2069
                     /locus_tag="KR314_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBH0329141.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            2112..2630
                     /locus_tag="KR314_002772"
     CDS             2112..2630
                     /locus_tag="KR314_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBH0329142.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            2766..3725
                     /locus_tag="KR314_002773"
     CDS             2766..3725
                     /locus_tag="KR314_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0329143.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNTRTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            3751..5454
                     /locus_tag="KR314_002774"
     CDS             3751..5454
                     /locus_tag="KR314_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBH0329144.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINRV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            5711..7813
                     /locus_tag="KR314_002775"
     CDS             5711..7813
                     /locus_tag="KR314_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBH0329145.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEAIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKNVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQINEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMHGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            7826..>8052
                     /locus_tag="KR314_002776"
     CDS             7826..>8052
                     /locus_tag="KR314_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBH0329146.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNE"
BASE COUNT         3258 a          791 c         1511 g         2492 t
ORIGIN      
        1 tttgttattt aatttaaaat atgaaaaaaa gtattataaa cacattagaa tacttctgga
       61 tttatgagta tacaatttga aaaatataaa aaataatgta taaagctaaa tctaatttaa
      121 tcgataatac atatagagaa atataattaa gtaaataaac aactatctat tagatattga
      181 gaaagtcaaa tcctatatga atctacagaa atgattttaa tgaatctgaa aaaatttttt
      241 atacaaacat aaaaaaggta taaagttgtt ttattataat ttgattactt ttgataactt
      301 tttatgtttt atacaattat atttttaata actcgtcaaa acgccaaaca tatattacaa
      361 tagatatata agtttttttg ttgacaaatt atgttaagag aattaagtat aatatggcat
      421 aaataattac ttttatttat caatgaaatg tatttattgt gtagcgaaaa atagtcagat
      481 agtatagaca ataactgaag ataaaagcgt ttattgttgt acatatggag gagttagttt
      541 tatggaaaat aaaattttga tagtagatga tgctattttt atgagaatga taattaaaga
      601 agctctgagt aaaagtggct ataataattt aatagaagca aatgatggag aagaagcgtg
      661 taatatattt aaatatgaaa agcctgattt gatgttgctt gatattacaa tgccaaaaaa
      721 agatggttta gaggtgctaa gagaaataaa aaagttggat tcaagtgcta aggttgtaat
      781 gtgctctgca ataggacaag agaatatgat tgtagaagcc ataaaacttg gagcgttaga
      841 ttttatagtg aagcccttca aaacagaaac attagttaaa gttgtaaata atgttttaaa
      901 ataaaagtgt aaatatacac tgatacaatg aaaagcaaat aaatgcaata ataaaaggga
      961 ggggtttata ttataaatta tttagaactt gatgatgttc atatagatgc tcttagagaa
     1021 attggtaata taggctctgg gaatgcaatt actgcacttg catccatgat aaatagcaat
     1081 gtagaagtat taatacctat ggtcaagata cttgaatata atgaagcgac aaatttgctt
     1141 ggtggtccag aaaacaaagt agtatgtatt ttactcgata tgaaaggtga tatcaatgga
     1201 atgtttatgt ttttgttgga tgaatcaata actcaactta tgctaagttc tctttttaat
     1261 aaagaagaag cttttttaga tgagattgag gcaattgaaa tttcagcaat taaggaaata
     1321 ggaaatatta tggcaagttc atatgtaaat gctattgcat ctatgttaaa catgactata
     1381 agtgtttcaa ttccagatat atgtattgat atggttggag ctgttctaaa tgtgcctatg
     1441 ataagatttt cagatgttgg tgacaaagtc ttatttatag aaaataaatt taaaatgagt
     1501 gataattatt ttactagcca cattttgatg attccagaaa tgagctcact tagagaaata
     1561 ctcgtgaggt tgggattaga agtatgaata gagagatagt agtgaacatt gcagatatga
     1621 agatagccta tagaccaaat gttttagtta cttatgcact tggctcttgt gtaggagtat
     1681 gtttgataga taaagtagct ggaattggtg gaatgttaca cgttatgttg ccatatagta
     1741 aagatgctat taacattgaa aataagtgta aatttgctga cacaggaata aatgagctca
     1801 ttaaatctat ggagaatata ggtgcaaata gaatatatat aaaagcaaaa attgctggag
     1861 gtgctcagat gtttttagga agcagcaatt cagtaattgc aagtatagga cataggaatg
     1921 ttgaggcagt aaaaaaaact cttttaggat taaatattcc aatattagct gaggacacag
     1981 gtatgaatta tggtagaaca atacgttttt acactgagac gggtgaatta cttgtagatt
     2041 caataaataa aggtaagaag aaattataat atatatttca tagaaagtgt attttaggag
     2101 ggaggtatta aatgagattt ccagatgacc catatttaga ttatggacta attgatgaag
     2161 gaattaagta tctaaagttt aaagtagatg agcaagattt tggggttgaa cttgaaaagg
     2221 taatagaaat tactggaaat caagaaatta tatttattcc agaactacct acatattcta
     2281 aaggcataat aaatctaaga ggaaaaatca ttccaataat agatatgcgt ttgagactta
     2341 aaaaagatga attaatgact acaaattgca tgtatatagt agtaactgaa ataaaggact
     2401 tggtagttgg atttgtggtt gatagagttg atgatatagt aagcttagaa aaaagtaaaa
     2461 tatcacctcc accaagagtt actatagaat attcggatta ttttgtaagt ggcgttggag
     2521 aattagaagg taaaataatt actttactag atttagagaa acttttgaca gataaagatg
     2581 aagttttaat agatgaatta atcaatgggt ttgattgcac tatcatgtaa catagggatg
     2641 tagttggagt gttattataa aaagattgtg atgagaacat ttaagaatat agtggtataa
     2701 ttattaagta taaaaatttt aaatcattaa aatttgaata tattatgttt aagagaggtt
     2761 tgtatatgaa aaataagcaa cagggaacaa ttttagtcgt tgatgatagt gttctgatgt
     2821 gtgacttaat agataaaaca cttacaaaag ataattttaa ggttaaaaaa gcatttaatg
     2881 caatagaggc aaaagaattt attgaagaat ttataccaga cattattttg cttgatataa
     2941 ttttaccaga taactctggt tttgagtttt gtaaggaagt aaaatctaat actcgtacag
     3001 cagatatacc tataattttt ataacttcaa aaaatacaga tacagacata gtaaaaggat
     3061 ttggtgtagg agcaattgac tatatggtga aaccttttag tatgacagaa ttaaaagctc
     3121 gtgttatagc tcatcttgaa gtaaagaaat ctcaagataa attaaaaaag ataaataatg
     3181 aactggaatc ttctcttgaa aaattgaata gactcgttgt aagagattat cttacaggtc
     3241 tttacaatag gcgacatata attaatcagc ttatggagca aagaagaatt aataaatata
     3301 gtgaaacatc aatatccctt atccttggtg atattgatga ctttaaggtt atcaatgata
     3361 cttatgggca tgaagcaggg gatttcgtgt taacagtaat atcaagtata ataaaaaaga
     3421 attgtagaca aattgatact gtatcaagat ggggtggaga agagtttttg ataatgctca
     3481 caaatactcc aatagaaggt gctaagatac tttcagaaag aatccgtaaa gagataaagg
     3541 agtttgattt caactataaa gaacataaaa taaagtgcac tattacatta ggagtagtag
     3601 aagtaaactc aaatatatca ttagaagaaa acatatcctt agcagataaa gcaatgtatg
     3661 agggtaaaaa cttaggaaaa gattgtagcg tagtgagtac aatgaaagga ttaaaaaaga
     3721 tataaactta ctttatgaag gagtacagat atgaaaagag aagtaaagct ttctaaatta
     3781 gcaattgtat tgctgattat tatagtattg gctgttctag cagggatttt ttcttctatt
     3841 tcatcttata aagcatttga aaaagtagaa aaaagtatta atgatatgaa taatttgagg
     3901 actcaaagtc agtatataaa agatacaact agagagataa gtgatgtatt aagaagatat
     3961 gtatttacag gagaaaaaaa gaataaagat atttatgaaa aagaattata cggaaaaaca
     4021 agagaatcag ctttaaacaa attagaaaaa attggtctta caaagagcga gtcaaataaa
     4081 atcttagaag caaaagaaat atcagattct ttagtaccaa cagaacaaag tgcaataaag
     4141 gcagtcgaaa gtaatgataa gagtaaagct caaagtataa tttttggtag tgactatata
     4201 aactcccaag agaaaatgga ttctttaata aatgaatttc aagaggatat agttacccgt
     4261 tcaaattcca aaattgtaga agtaaaaaaa gaattacaaa gtctaatatt aagaacaata
     4321 tttatacttt cccttttaat aatattgaca ataatagaat atacagtaat aaaattaaag
     4381 atagtgattc ctattaatag agtagaaaaa cacttttcaa gaattgcatc tggggactta
     4441 agaacaagta tagacgttga agaaagtaaa tctgaaatag gactattagt gagttctgtc
     4501 aaaaagatgc aaagtatgct tttaaattac atagacctta tagacagtac attaactagt
     4561 attgcaaaag gagatttaag agtagagata gaccaagaat ttataggtga ttttaaatca
     4621 attaaaatat ctttagaaag tataataaac tcttttaatg gtgatttttc tgaaataaat
     4681 ttatcagcag aacaagttgc aagtgcttca gaacaagttg cagcaggtgc acaagctctt
     4741 tcacagggtg ctactgaaca agctagttca gtagaagaat tagcttccac gattaatgag
     4801 atatcagatg gaattaagaa gtgtgcagat agtgctagtc ttgcatgtga ggtatctgac
     4861 caatctgcat cacaagttgg ttttggaagt agatgtatgg atgatatgat gacatcaatg
     4921 ggtgaaataa gtgaaaaatc gagtgaaata tcaaaaataa tcaaaacaat agatgagata
     4981 gcattccaaa ctaatattct cgcattaaat gcagctgttg aagcagcaag agcaggtcaa
     5041 tatggtagag gatttgcagt tgtagcagat gaagttaggt ctttggctgg taaaagtgca
     5101 aaagcagctc aaaatacagc tattctcata gaagaagcta ttaaaacagt agaaaatggt
     5161 gtcagaattg ctaaagagac tgcaaatgca ttggacttgg tagtaaatgg tgtagaaaaa
     5221 actactaatt atattaatgg tatttcttcg gctgtacata gtcaagaagt agcaattaca
     5281 cagattttat ctggaataga gcaaatttcc atagtagtcc aaatgaactc agcaacagca
     5341 gaggaaagtg cagcagcctc agaagagatg tctgctcaag ctcaaatttt aaaagaatta
     5401 gtagaaaaat ttcatctaaa ggaaagtgaa gtaagagaat catcatgggt ttaaattatg
     5461 aggtgaatat tgaagtaaga gaattatcat gtatttaaat tatgaggtga atattgaagt
     5521 aagagaatta tcatgtattt aaattatgag atgtatatat attgaaataa gagaatgatt
     5581 gtgtatttaa attatgaggt acatatagtg agataagaga atcatcaagg atttaaatta
     5641 tgagatgcat atataataga aaattatttg taggtcgtag gtgataaatg tgaattatat
     5701 tgaaccgagt atgagacaaa tgttagatat gtttatattt gagacaagtt tattactcga
     5761 acaattagat gaaattatga ttcaaataga aaaagagaaa gtatttactc ataacaacat
     5821 taatgaaact tttagaataa tgcatacgat aaaaggttca gcatctatga tggggttaga
     5881 caatatatct gctttagcac atagtgtaga agacttgttt tatgctatac gtgaaggtaa
     5941 aatagaagtt ttagacaatg acctattatt tgatttagtc tttcaatcat tagattttat
     6001 aagaagagat atagatgagc tacaatcaag aggttgtata tcttctgatt actctaattt
     6061 tattgaagga attgaagagt atgtgagtac tttggaatta tcaagttcta tgaaatcaaa
     6121 ggataatgca aatttagaaa gtacagacga aaaagaagca atcgattcaa attttgaata
     6181 caaagaagac tctgatgtag taaaaataat ctttgaacaa ggttgcatga tggaaaatgt
     6241 gagagcgttc atgcttgtat ttgaacttag agattactgt gaatttatag atttttatcc
     6301 aaaagatgta gaaacaaatg cagagtctac agaatatatt aaaagttatg ggttttatat
     6361 acaatttgta gctaaagagg ataaggcaat agtttataaa actatagaag ataatctcta
     6421 tgtaaaagaa tacataaata ttagtaaaaa agaaatagaa atgaaaaata catcaaataa
     6481 tgatgcagta gaaaataaat taaaagataa taataaagta agtaaaagtt atcaaaataa
     6541 ctatacagaa gatgagatac aagaagatgt tttaaatgaa aaaactgata tggaaaaaga
     6601 tagtctaagt gaagaaaatg aacatttacc aataagtaag cagagtatta tgagtgtaaa
     6661 tttagaaaaa ttggatttac ttcaaaatat agttggagaa attgtaatta tggaatcaat
     6721 ggttgtaaat agcgtaaatt ctacaggtgt tgatatagat aatttcaata aggcaacaag
     6781 acaattacac aagcttacag atgaattgca agatatagta atgtctatgc gtatgattca
     6841 aattgctggt gtatttcaaa aaatgcatag aattgtacga gatatgaata aaaaattaga
     6901 taaaaatgta gagttaataa ctataggagc agagacagag gtagataaga atgtaattga
     6961 taatttagca gaacctttta tgcaccttat aagaaatgct atggaccatg caatagagcc
     7021 tattgaggag cgtatagcga aaggtaaaga agaagtggga aagatagttt tatctgctga
     7081 gaatctaggc ggagaagctg tgataagtat ttcagatgat ggtagaggca ttatgagaga
     7141 taagattttg gcaaaagcta aggaacaagg tcttcttaaa catcaaatta atgagtatac
     7201 tgatgacgag gtcaattctt tgattatgac tccaggtttt tctacaaaag aatctataac
     7261 tgaattttct ggaagaggag taggcatgga cattgttcgt aaaagtattg aaaaagttgg
     7321 aggttcaatt gaagtaaaaa gtaaacagga tgaagggaca acctttacta taaaaatacc
     7381 attaactctt tctatagtag agggtatgaa gtttaaagtt ggagattcac tatttacttt
     7441 accaatatca tccataagaa gaacatttaa acttacagac ttaaatcaaa taattacaga
     7501 aggaaataag acagagttaa ttacaatata tggagcatgt attcccatta taaggttaca
     7561 taaaatatat aatattcaaa caaaagtaac caatctgatg catggtaata tgataattat
     7621 tgaaaatgag cataaagcag tatgcatttt tgttgatgaa ataataggta atcagcaagt
     7681 tgttgtgaaa ccatttccag tttatttcaa ccgatttaat ataaaaaata agggtttatc
     7741 gggatgtaca atacttgggg atggaagtat aagtttaatt atagatgcag atagcattat
     7801 agaaatatat taggggattt aaagtatggc taaagatgaa attattaaac aagtattaac
     7861 attttatgta aatgatgtta tttatggaat agaattagaa aatgtgattg aaaccataag
     7921 atttcaatca ataacgtatg taccatgtct tccaacatat ataagtggag ttattaattt
     7981 gagaggaaga ataataccag ttattaatat gcatataaag tacaatcttc ctaaagctga
     8041 ttataatgag ag
//
LOCUS       DAEPLF010000158         8015 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEPLF010000158 DAEPLF010000000
VERSION     DAEPLF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8015
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.158"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..783
                     /locus_tag="KR314_002777"
     CDS             <1..783
                     /locus_tag="KR314_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBH0329147.1"
                     /translation="IVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIIIV
                     IILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDIKRFKAIIA
                     TSLVLGVLGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTFE
                     SFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYEE
                     FDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            898..1683
                     /locus_tag="KR314_002778"
     CDS             898..1683
                     /locus_tag="KR314_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBH0329148.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDGLEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            1975..3294
                     /locus_tag="KR314_002779"
     CDS             1975..3294
                     /locus_tag="KR314_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0329149.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMSIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(3369..3797)
                     /locus_tag="KR314_002780"
     CDS             complement(3369..3797)
                     /locus_tag="KR314_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329150.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(3888..5264)
                     /gene="hydG"
                     /locus_tag="KR314_002781"
     CDS             complement(3888..5264)
                     /gene="hydG"
                     /locus_tag="KR314_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBH0329151.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(5265..6350)
                     /gene="hydE"
                     /locus_tag="KR314_002782"
     CDS             complement(5265..6350)
                     /gene="hydE"
                     /locus_tag="KR314_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBH0329152.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSPDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNKDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(6386..7594)
                     /gene="hydF"
                     /locus_tag="KR314_002783"
     CDS             complement(6386..7594)
                     /gene="hydF"
                     /locus_tag="KR314_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBH0329153.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVNKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(7609..7860)
                     /locus_tag="KR314_002784"
     CDS             complement(7609..7860)
                     /locus_tag="KR314_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBH0329154.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         2855 a         1107 c         1167 g         2886 t
ORIGIN      
        1 atagtagata gtaatcttga ctttatgaaa gctactgtaa atattataga aaatataggt
       61 atatttattc ctatgggaat ttttttacct atagtatgta aaaatctaaa taaaaaaact
      121 ataattatag taattatact tgttagccta gcatttgaat taacacaata tatttttgct
      181 cttggaagta gtgatataga tgatgttatt ttaaattcat taggtggaat aattggaatt
      241 aatatttaca taaacatgaa taaattattt cctaatgata ttaaaagatt taaggctata
      301 atagctacta gtttagtttt aggagtttta ggattaggag ttatttctaa aaattatcat
      361 aacttactaa cttttaaatt tagacctgat agaaaaatat caaaaatatt aattgaagaa
      421 aatagggaaa taattaaaga tataaataaa gatacagtag atatagttgg gacatttgag
      481 agctttaaaa aaggaataat aactattaaa gcaggttcta ataacaaagt aaagagacca
      541 gagaatttaa ttgattctga tggaaatata agaatttatt taaatgaaaa tacaaaatta
      601 gtatcttata taattaccaa agaatcaaaa atggatatag ttaaatatga ggaatttgat
      661 gtgaagaatt tagatttatt aaggagatat gatactatta atgtttggat tgataaagaa
      721 aatcagccaa aggataaaca tggtattatg gctagtaaat tattaattgg attgtatgaa
      781 taaaatatta aacaagaata aaagaataac aaatataaaa aacgaaaaaa ctatttctat
      841 aattattttt acgttgtgca taaaataaga acattagtga aggagtagag ataaattatg
      901 gagataataa aatatatctt gtactttttt atatattcat ttttaggatg gacagtagaa
      961 agtataggat gttctatagc aagcaaaaga ataatcaaca gaggtttttt aaatggtcca
     1021 atatgccctg tttatggttt tggagcagta attgtgatat cactattagg aagatttaat
     1081 aatgtagtaa tagtatttct attaggaatg attttaacta cgatactaga atattttaca
     1141 ggatttattt tagaaacctt atttcatgct aaatggtggg attattctga cagaaaattc
     1201 aatataaagg gtagagtatg tttaaaaaat gcaatatact ttggtgtaat gtctgtttta
     1261 ataataagat ttatacatcc atttataaaa tattttgtaa gtataattcc atatagaata
     1321 ttaatttcaa tggctataat tacaacactt tggactatac ttgatttgat tgttactatt
     1381 ataacactta aaaaattaga tataaagtta aacttactag acgatataat tacagacctt
     1441 aatgatataa atgtgaaatt ggataaattt gatagaggag aaatacaagc tttatttaaa
     1501 acaataaata acgacggatt agaagttaga gaaaaaataa acaaaataaa tagtaagttg
     1561 gaccgtatag agtctaatat tattttacaa aaaagagtta taaaagcatt tccacatata
     1621 aaacataaaa aacaacaaga gcaactagaa cattttaaaa aaattattaa tgagaaaagg
     1681 taattataca atttaatgaa gacgaataga ataatggtat ttataagatt attttcagag
     1741 gactctataa aaatagaata gagtccttta ttttttgaaa aataatgttt atttgataaa
     1801 aataaaatat attgtttcta aataacaaat attttgaaaa ttataaaaac atatattgac
     1861 aatttgtgtc aaaaatacta acattaagta tataaagaat tttatatgag aatatcttta
     1921 taaatcgcaa taattttaga acataaattt atttaaagag gggatgattt gccaatgagc
     1981 gagataacta gtcaaatgat aagtacagaa gaaaaaaaat atgttgctaa aactcaaaag
     2041 ataccttatt atccagtagc atttaaatca ggtgatggtg caatgctata tgattatgaa
     2101 ggtaatgaat atgtagattt tttagcaagt gcaggctctg caaatgtagg tcatggtaat
     2161 aaggaaatct cacaagcagt taaagaacaa atggatgata taacacaata tacacttgca
     2221 tatttccata gtgatcctcc tgtaaaatta gctgagaagc ttgtggaaat agcccctgga
     2281 gataatgata aaaaagtact atacagtgca actggttctg cttgtataga tgctgctata
     2341 aaattagcaa gagggtatac tggaagaaca aagataatat ctatgtgtga atcttatcat
     2401 ggaagtactt atggagctat atctatatct gctttaagta caaatatgag aagaaaaatg
     2461 ggtccattgt taccagaagt ttatcacttc cattatcctg ataaaaatag aacagctaaa
     2521 gaatgtttag atgagataga atatgctttt gcacactatc taccagcaga agaggtagct
     2581 gcaatattta tagagccaat tgcaggagat gcaggaataa tagtgcctcc agtagagtgg
     2641 gtacaaggtt taagtaaaat atgtaaagaa aatggaatat tacttgtaag tgatgaaata
     2701 caacaaggta tgggaagaac aggtaagtgg tttggtatag aaaattttgg tgttgaagca
     2761 gaccttatag ttcttggaaa atctgttgga ggtggattac ctttaggagc agttgttgga
     2821 agaacagaaa taatgcaaag tcttgatgca ccagcacatt tatttacttt agctggaaat
     2881 acaacagtgt gtgtcgctgc tttaaaatct atagagataa ttgaaaaaga aaacttactt
     2941 caaaagagta tagaaatggg agattatatt aaagcaggat ttgaaaaact aaaagaaaaa
     3001 tacgatataa ttggagaaat aagaggaata gggttatcta taggagttga tatagttaaa
     3061 ggaaaaggtt ctaatgaaaa acatccagat gctacagcta agatatgtta tagatgtata
     3121 caaactggat taataatgat attcttagga caatctactt taagagtaca accacctcta
     3181 gtaataacta aggagcaagt agataaagca atgagcatca tagattctgc aatagatgat
     3241 tatttaaatg gtagaattgg tgatgaagta tatgaagtaa ctcaaggttg gtaaaataaa
     3301 tattaaatga aaaaaagagt tatctcaatt ttagaaagta atttctgaaa tgagataact
     3361 cttttaagtt acccacaaat aagaaaattg tttgattcat taaagactat atttcggatg
     3421 tcgtttttgt gagtatcaga gacagtatta cgagaataat tacctttatt cgtactatct
     3481 gttaatttag ttataattaa gatagctaaa agaataagaa ttactgtgta taaagcaata
     3541 acatatggtt ttcttttata tttgtatttg ttattgaaaa aagaatggcc ataattattt
     3601 ttatttactt tagatgtatt tgtattcttt ttcttgttac ctttcttttt atcatccttt
     3661 tttctatttt tagggtaagg aatgactttg tgtgtattat caagtttgat atttttattt
     3721 ttttgaatat tgttttccag gctagccttt ttttctccaa attgaatagt tttattggta
     3781 ttgttgttat ctttcaatgt taatctccct cccttaaact ttccctttag ataatattac
     3841 tactaaccta tggaatatac aataaataat atataagtaa taaaatttta taggtataaa
     3901 tctctttctc cacgctctaa ggcagtcata ttgttagata ctatgtttct tatatcttct
     3961 ctttctatct tacctatttc ttctgcgata agttttttac ccataatttt aagttcttca
     4021 tctccataat cgattaaaaa ttcatttaat gtagtaagag catttggcgt acatacatta
     4081 tggatttgtc gacttttagc taagctcatg aatctatcgc cagttctacc tttacgataa
     4141 caagctgtac aataactagg tatatatcca tcagtaatca gttctttaag cacctcaaca
     4201 gggcttctat ggtctccaac ttcgaattgt tctgtattgt caccatcttc atatgcttta
     4261 tatcctccaa cacctgttag agaacctgca ctaatttgtg atacaccata ttttagtaac
     4321 tcatttctca tttcagcagt ttctctagtg gacattatga ttccagtaaa aggaacagcc
     4381 aatctagtta tagctactat ttttttgaac atatcgtcag aaactaagtg aggaaaatct
     4441 tctaagctca taccttcagc ttttttcaat ctagggaaag atatagtatg gaaacctaca
     4501 ccaaattttt cctctaagtg ttcgttgtgc atcataagtc ctagaacttc aaatttaggg
     4561 tcagataaac caaataaaac tcctgcacca acatcatcaa caccagcttc catagctcta
     4621 tcaaaagctg ttaaatggta ataatagtca ttttttatag attgaccatg catttttata
     4681 aatgttggtt tgtgataagt ttcttggaat aatatataag ttcctattcc tttttctttt
     4741 agtaatttat actcatctac acttgtagct gctatgttaa cgtttaccct tcttatattt
     4801 ccgttttcat tatatgttga ataaattgca tctaagcaat ctaagatgta atttatatca
     4861 caatttttag ggtcttcacc agcttctaga gcaagtcttt tatgacccat tttttctaat
     4921 attttaacct cttcctttat ctcttccatg gtaagttttt ttcttttaaa tttgttgcat
     4981 ttactaaaac cgcaataaac acattcattt acacagtagt ttgatacata aagaggggca
     5041 aataaaacca ctctattacc atatatttca ttttttattt gcccagctat actaaataat
     5101 ctatctagtt gttttttatc ttctacttca agaagtgtag ctatatcttt ataactaagt
     5161 ttttcccttc tatcagcctt atcaagtacc ttttcaatat catcagaagt tgaatttttt
     5221 gcatcttcta ataaagaatt aataagttca tggtttatga acatttacaa acgcctccat
     5281 tcaatattat caccgcgaga atagtcaact tcaagtccta atgacttgat gttttcttct
     5341 aaactttggt gatattcaca agcttcttga ccagtaaagg ctttgttatc gtaaagagaa
     5401 tattgttttc taaattcttg aggagaaagg tttggcataa ttacattgca accagctaaa
     5461 agacctgcat ttctaccact agggtttata gatgccaagg ctgttgtagc gggtaataaa
     5521 acttttggaa gtagaagtct tgtaattgaa agcataagta atgtcttctc taaatctcca
     5581 tgtttataat ccttaaaaat tgtatcatgg tgtggaataa aaggtcctat tccaaccata
     5641 tgaggattta actcttttag gtataataaa tcacgaacaa ggtctttgtt gttctgaaat
     5701 ggtgattcaa ccataaatcc agcacctacc tgataaccaa tttctttaag gttttttagg
     5761 cattcttttc tagttctaag ctcaatgttt ggcggatgta attttttata atgactgtct
     5821 gtagcagttt catgtcttaa aagatatcta tctgcaccac ataaaaagta ttttttatac
     5881 gaatcataac tcttttctcc tagtgacaga gttatggcac aatcctcata tttatttttt
     5941 atacttgaaa caatttcaca tattttatca tcagtaaaat atgcatcttc accaccttgc
     6001 aaaacgaatg ttctgtatcc tattttataa cctatatcac aacattcaag tatttcatca
     6061 ggtgaaagtc tgtatcttat gatattttta ttagaagatt gtattccaca atagtagcaa
     6121 ttatttttac aatagtttgt aagctctata agtcctctca agtagactct atttccataa
     6181 tgtttatttc tcatgtcgct agcttgttta taaagatagt ttcttaaatt ttgttcgaag
     6241 ttatattcct cattgtcgat attttctatt aaatatagta gttcatcaaa acttaatgaa
     6301 ttaatgctgt aaagtttatc tataaagtat ttaatcttat ttttagtcat attcaaatat
     6361 gtaattatta agtatagtta aatttttaat aattgaaaag ttcaagtgat ctttccaaaa
     6421 taccttttac atatgctatt aagataccat agtttacaat aggtacattg aaagattttg
     6481 ccttttctat tctagataac attccagctc tattcatcat acaagctcca cagtgaacta
     6541 caagggcata tttatttata tcttcagtga aggatactcc agaactaaat tcaaaagtaa
     6601 tatttttgcc agttttcttt ctaatcatat taggaatttt tactgttcca atatcatcag
     6661 tttgtctatg atgggtacat ccttcagcca tcaagatttt atcaccatct tttaaatttt
     6721 ctaaagcata agctccatta atcaattctt taaggtctcc cttttgtcta gcaaacaata
     6781 tagaaaaaga tgtaagagga atatcttttg gtgtatcttt atttacttgt ggaaatactt
     6841 gagagtcagt aataacaagt tttggttttt tgcctaaatt agatagagtt tcttttaaac
     6901 tatcttcttt tgtaactata gaaattgcac cattatccaa aatatctctt ataacttgct
     6961 gttgaggaag tataagtcta ccttttggag ctgctttatc tattggaaca actaaaacaa
     7021 ctaaatcatt tggttcaatt aaatctgata ccaatttgaa ctcagtgcta tccttaggca
     7081 aaacttttat tatttcattt tttaaatttt caattccaat tttatctgtt gatgagacag
     7141 aaacaactgg acatttaact ttatttttag ttaagtttaa aatttctgat tgattcttaa
     7201 tagtatcaat tttgttgaga attagaatat gagggatatt cttatcatta aacttttcaa
     7261 ttagtgacaa atcttcttgt gatataccaa tttggcaatc cactactagt aaagctatat
     7321 ctgtcttttc taaaacatct agtgatttac ctattctaag ttcaccaagt tcaccaacat
     7381 catcaagacc agcagtatct ataagaacac aaggtcctat aggaaggatt tccattggtc
     7441 taaatactgg gtctgtagta gttccagcaa tatctgagac tattgcagca ctttgatttg
     7501 ttatagcatt tattatgcta gatttacctg catttctttt accaaagaga cctatatgta
     7561 ctcttaccga ttgtggagta gaatttagac tcatttgtac tcaccacctt aaacttcttt
     7621 ttttgatata ctagttttta cagatacatg aggtatgttg cccaatttac cagtcaaact
     7681 attaatagta tccatttcgc caacaactgc tatacatacg attgagacac cttcttcttc
     7741 gcaaggaatg cccattcttc ctttaataat acctttaaaa cctgatacaa cctcattgaa
     7801 tttaaattga cattcttttg gctcttccaa aatagcactt ataactgcaa cctttttcat
     7861 aaatatcctc ctaactcaga aaatatgtct taaacaaatc caaaatcaaa aaacttttct
     7921 ttgtaaagaa aagtttctac aaaagaaaag ttattaatta cacaagtata atgtgctata
     7981 ttaaactatt tccttacaga ttagataact ctttt
//
LOCUS       DAEPLF010000159         7966 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEPLF010000159 DAEPLF010000000
VERSION     DAEPLF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.159"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..1826)
                     /locus_tag="KR314_002785"
     CDS             complement(<1..1826)
                     /locus_tag="KR314_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329155.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCNMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAKDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNQLDIDVEWDFTDYYEKLEKGENFDITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKTIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNSDEKQPNTPKDD
                     VESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNPITENDDNYDLSSKSVFGTNEH
                     SFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNL"
     gene            complement(2010..3485)
                     /gene="spoIVA"
                     /locus_tag="KR314_002786"
     CDS             complement(2010..3485)
                     /gene="spoIVA"
                     /locus_tag="KR314_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBH0329156.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(3657..4952)
                     /locus_tag="KR314_002787"
     CDS             complement(3657..4952)
                     /locus_tag="KR314_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0329157.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNKSNENTTKVS"
     gene            complement(5136..6545)
                     /locus_tag="KR314_002788"
     CDS             complement(5136..6545)
                     /locus_tag="KR314_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0329158.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(6597..7736)
                     /locus_tag="KR314_002789"
     CDS             complement(6597..7736)
                     /locus_tag="KR314_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0329159.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
BASE COUNT         2511 a         1472 c          866 g         3117 t
ORIGIN      
        1 tttagattat attctttaga ttctaattta taattattat ttattattcc atcataatca
       61 cttatatttg acttattttt atttgtatct tgatttttgt tagaaatact ttccctataa
      121 ggaaaagaat gttcattggt accaaatact gattttgagc ttaaatcata attatcatca
      181 ttttctgtaa ttggattttt attgtaaaat cctttttctt tatcagaatt attttcttta
      241 ttaccgtctt taggtatatc atgaggattt ttattttcat tgctatcagt acttgattct
      301 acatcatctt taggagtatt tggttgtttc tcatcagaat tattattttc attattattg
      361 ttttgattgt tattactatc attattttca tttccattat tcccatcttg attgttatca
      421 tcctcatctc tatccgggtc gtctctacct cctcctccac ggtcaccatc aatatcatca
      481 ttttcccgtt catcatctgg cgtctcttgg ttgtctccgc ctcccattat aaatcctggt
      541 ttaaaaacta tatttgaata cccttctttt aatcctccta ccacttttac tctaaaacaa
      601 cgcaacttaa aatcttcaaa aaaaagtata gcttgatctc taatatcctc ctgttcttca
      661 gatatccaat tgattccatc gcttgaatat tcaacaaaaa ccttacttgc actatacggc
      721 atatcataaa ataaaacagc tacatatcca ttttttgtat tttgaaattc cagttccaaa
      781 ttgtctattg tttttttctc tataacagat acattcaaaa taggaactac atcatcattt
      841 attataattt tttctttatt taacatttct gttttaaact ttcctgttat atcaaaattt
      901 tctccctttt ccaacttttc ataataatca gtaaaatccc attctacatc tatatcaagt
      961 tgattaccac tagatgtttc taataccata tatttgggaa attttatatt ttctatttta
     1021 tcatctttac ttacttttat ttcatcacta aatacaccaa ttcccctatg acaacatttc
     1081 acaatattat agccagaatc tttagctata gagggtataa gctcattata atcatcaatt
     1141 atataaactt tcttactttc tgatataatt gattgtgcca acttattatt atctcttagt
     1201 aacccacttt cattgttgct actatatgct tttattgaag tttcttctat ctcaacatca
     1261 cctttagaat aaacacctat agcatgttct ccattcactt caatatcctt atttagtatt
     1321 ctaatatcat tttcagtata aattcctatt gcatcttttc catttgcctt tattttttca
     1381 acagattcgt aggatgattt aatatgtaag tctcctcctt cttcaacgta taaggctgtc
     1441 ccatcagtag caattatgtt gtttatacca atacttatac ttccaccatt ttctatatgt
     1501 attactcctt cttttcctcc ttcacctatt atattcaatt cattatcatc aataataaat
     1561 ctcccatgat tttttatcaa tattttatat ttgttggtat ctattgtaaa attactgtca
     1621 taaggaatat aaaatctaaa ctcttcatct attaccatat tacaagaaag agtataaact
     1681 atttcttctt tattattttt attttcttga acccaccttt caaaatcttc aagacaattt
     1741 tcctcttgtc caaaaacttt atgtggaaaa cttataaaac acaaaactat tatactcata
     1801 aaaataaata tctcatactt tttcatctta cactcctaga aattttttag ctttgaaaca
     1861 atcctgtcga aacatatact tcacccacta taatttttcc acaattatca attattgtca
     1921 atttatataa gtatctttat taattgttat aaataaaaag ctacttacta aagtaaacag
     1981 acgtttcact ttaataagta gctatatttt tataacaaaa tagttataat attagaactt
     2041 ccttcattta caattttttg taaagtcttt tgcattttaa ctcttatctc ttctggcata
     2101 gtatataatt tactttgtaa ttgctctttt actaagtcat gtagtgactt accaaacata
     2161 tttgattccc ataaatctgc tggttgctct tcaaatactt ccataagata ttttatcatc
     2221 tcttcacctt gattttgatt tccaactatt ggagagacct ctgtagatat atctgctttt
     2281 ataatatgaa gtgatggtgc attggctttt aacttgatac catattgttt tccttgtttc
     2341 attatttctg gttcttccag actaagttct tctaaagaag gagctactac tccatatcct
     2401 tttatcttgg catctattaa agcactttct attttatcat actcattttt aactttagat
     2461 agtctagtta tcagactaag cagttgataa tctccttcaa ttttaaatcc acttttttct
     2521 tcaaggacat tatagaataa atcttgcttt gtctgtaaat caatatttat aacaccttct
     2581 cctagctcta cattatcaac acctgtatct tccaaaaact ctaactcaga aaatccttgt
     2641 ataatacctt ctatatctct tattttacct atatctataa tactttgttt taatgttgtt
     2701 attatgctac tttttatcca atgatttctt tcaagacctt caacccattt tggtaagttg
     2761 attcttattt ctgtaagtgg aaaatcatat aatacagctt ccataacctc ctctatatct
     2821 tcttcttcca tttcaaccac attcattgga agaactggaa cttcatactt ttcttccaat
     2881 tcacttctaa gcatactcgt ttcctcactt tttggactta acgtatttaa aaccactgca
     2941 aatggttttt taagattttt caattcttgt ataactctct cttctggttc tacatagctt
     3001 tttctgtcaa ttccagtaac tgaaccatct gtcaagacaa ctatccctat tgtagaatgg
     3061 tcttttataa cttttttagt tcctatctct gctgcctttt caaatgtcat agcttcttgt
     3121 gaccaaggag tggatactaa tctttgtttt ccaccttcct catgccctaa agctccttca
     3181 actatgtatc caacacaatc taccattctt acttttaaag atactgtgtc ttttattttt
     3241 atttctacac catctgctgg tacaaatttt ggttctactg tcattatcgt tttacctgaa
     3301 ccactttggg gtatctcatc tcttgttcta tcctttttaa attcattgtc tatattaggt
     3361 ataaccaact tttccataaa ttttcttata aaagttgatt ttcctgttct tacaggtcca
     3421 actactccaa tatatatatc cccctgagtc cttttggata tatcttcgta tatgttatta
     3481 ttcatattat tcctcctata catctaatct tattaaatta tatttagagt taagggttaa
     3541 tattcttaaa atagaaaaat aaagaccagc aatattaatt taatttacat atagaaattt
     3601 gtaaatttca atgtaaatta aattaatatt gctggtccaa atagtagtgt attatattaa
     3661 ctgactttag tagtattttc atttgactta ttattcatct caataagttc tttacgtctt
     3721 tcttttgtca tgttcatcca tataaatgta aacaccaatc caattatggc tactatagta
     3781 agaactatga agtagtacct atatccagtt acacccggaa atgcatccaa tatcttccct
     3841 gctgctaaag gacaaagtac ttctggaaga tatcctaatg tagcaactat tcctatagca
     3901 gttcctgata ttgataatga ataattacct tcttcaagta acgaatagtg taaaccttga
     3961 attgcataca tagatacata tattgcaaca catccaatca atagtatata aatcatagat
     4021 gattttgttg gtgtaaatat tacacctaaa agacctgcaa tcataagtat aaatccataa
     4081 gatataatct ttgatgaacc tactttatct ccaagtaatc cagaagcagc tgatgctact
     4141 ggtctacaat actgagctaa tattgagatt gcagatgcaa acaccacagt agctccaaat
     4201 gcttcagttg aataaggcgt aaaataataa aatgacatat tagctgaata tgaacaaaac
     4261 attattattg ttattatcca tgtatgaggc atttttatta ctgcaaaaaa ggcttcttta
     4321 tcaaattttg ctgatacctc tttttcatta tcctttaatt tgaaaaatac taatactcca
     4381 actattacat ttattgccga gtataatact ataatagctg taagaccaga tttatcactt
     4441 acttttgatg aaatataccc aaatagtatc aatactagag atagatgaat tgcattggta
     4501 attccacgac cactttccat aaatccaaaa gcttttcctt gttcattttc agatgctaac
     4561 attctaacag cttttacaag agctggccaa aaagtaagta ttgttgttat tccccataag
     4621 gcatggatta aaaacacaac aatatatgga ggataaagtg ttaatacaag accagatatt
     4681 cctgtaataa ttaaagaacc tgaaagcaat tttctggctg aaatacggtc tgctaaaaaa
     4741 ccaccaaaaa ggtatgatac cattgcaagt agaccataaa tgcttcctaa gcttcccatt
     4801 tgtgtatttg taagattaaa tgcttgtgca aaagtatcat aataataatt ctttaaatat
     4861 ggtaaagcat atataaatgc tcctgctaca aagagtatta tcaagacttt gaaatttttc
     4921 caaatgcttg gtttatttac ttcaactgac atacttcctc cttatgtaaa tacctgtaaa
     4981 ttttcatgat aattaattat tgcttatttt attgtttatt caatatttcg gaatatttat
     5041 taatatatta ggttaatcta tcaataattt atggtgagtt atgagctaaa agtaaactct
     5101 tttagctcac cataataatt atatttttat ttatactaac gtccagagtt attagatatc
     5161 tttttttcat agttcttcca tagcattaaa cctgttatta taacaaaaag acctcctcct
     5221 atcatttcaa tctcatagat tatattatct tttaaagtct ccataactgg tgccgttgag
     5281 aaaagtatgc caactacagt tactacaagt accattctgg caatattgat acaaactgaa
     5341 tcactttttg caattgaata tggtctttct tcatttggtc ttgtctttct aagtctaata
     5401 taagatgtaa aaagcaagac atatggaaat aatgctgtta atgctgtcat agtaacaaga
     5461 acattgtata tattatctac tgatggtaat aagtttgttc ctacaagaag tagactcact
     5521 attatagcct gaactattac tgcatttgat ggtatattgt atttatttgt aacagtcaat
     5581 tgcttaggaa atacaccttc tcttacattt ccaaaaagca tctttactgg acctgctata
     5641 taaagtatta atgctcctag tactgataaa gttattccaa atgctactat tcttataaac
     5701 caacttccta tacctaaatc ttgtgcaact ttagctaatg catctagtat ccctgtagac
     5761 gctgcaattt tatctggagg aagtatcatt gttattgcta ctgaacctaa gatatagatt
     5821 cctcctatta aaacagccac agttaatata gctttaggaa agtttttctt agcattatcc
     5881 atttcggtta tgaaattagc agtcgtttca gctcctgata atgcaaatat tatagcagat
     5941 atagaaacta atgaattggc atctattttt ggtattatat tttggattgt atatatcgat
     6001 gctgatggag cttttttgag aataactaca gacataaatg ccatcactat aagtaatata
     6061 gcaggaattg tagaacctaa tgctcctgta ttcgtaaaaa attttccaaa tgccatccca
     6121 cgagtactta ctaaggacaa cagccaaaat attatgagtg aaagtgctaa aataaacatc
     6181 ttattgtttg ataaatctgg atttccaagc atatatgcaa tatttatagc taaaaatgtc
     6241 aagaaagaag aataccaaaa tatctttgat gtccaattta gccatgacac cataaatccc
     6301 cacttctctc cataggcttg tttaacccat tctccaagtc caccatcttt tggatatgtt
     6361 gcagccaact ctgcacatat aagtgatgca ggaacaaaga atataaaagc aaacaataac
     6421 caaactggta tagaaccaag acccaatcca aaactagagg cagtagattt agctatccat
     6481 ctaatcccaa atagtgctga tatattcata aatactaaat cccaaaatcc aattttcttt
     6541 ttcactgtaa ttcctccaat ttcaaattta ttcaaactca tacacaatgt attgatttaa
     6601 atatcaagta taagttcttc aacacttctt ggataattag ttataattgt agcaccattc
     6661 ttacctacta ttgctgtatc tgagtgtcta aatcctccaa ctccaggtat atatatccct
     6721 ggctccatag aaaataccat tccttcttct aatattaatt cattgtcaaa tcttaaatat
     6781 ggctcttcat gttctgataa acctaaacca tgaccaattc tatgatttga atataactct
     6841 agaccatact ttgcaacaac agctcttgct gcttcatcca ccattcttgc tggtatacca
     6901 gcttttattg tatctaatcc agcttgttga gcctctactg caatcttaaa cacttctttt
     6961 tgtctttctg ttggtttacc tataataaaa gttctttcat tttctgctct atagttttca
     7021 taccaaacct gtctactatg tattactata tctcctcttt gaagtatcct agtattggaa
     7081 tctaaatgag gttgtgctgt tctatcaatc cctgaacaag tccagtttgc aaatcctata
     7141 tatgtatcag gataattttc agaaacatat tttagtaaag cattatcccc cgcaacgtca
     7201 aactccaatt cactcatccc aactctaaca ttttctaaag acccttttat tcctatatca
     7261 gataggtaac ctgctatctt caaaaattct atttcttttg catcttttac atatctcatt
     7321 tcaaatattt tacttccaac atctttgata gcaaatgact tatcagttat atactctgaa
     7381 aaacttgcag atactatatc tttttctatt ccaacagttg ttcctttagg gtatttattt
     7441 aaaattatat ctaagtaatg tttatgtgat attccatgtt ctttcatttc tggtatttca
     7501 taatacacat atacattatc aacttttgca acttctttag catgtaattc ttctaatcca
     7561 ggtactatta attctatttt atcttgagtt actactgtta caattggtct agaataagtt
     7621 attgctttaa atccactaat ataaaattga ttgtctggtt caaatattaa agcaatttca
     7681 atgctttttt cattcatgta ctttctcaat ctttctagtc tcatattatt tgacataatg
     7741 cacctctttc ttaatattca gataatttat tcaatacaac aaggataaat atattatttt
     7801 aaaattctta atatttttag tacttgtcgt atttgcatgt aaattaattt tatttgtgat
     7861 tattttatca taatcatgat tattttgtca atcacgattt tatttttaaa atttttttat
     7921 attctagtga ttttaaatta tctaaaatat aacgagtata cattgt
//
LOCUS       DAEPLF010000160         7898 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEPLF010000160 DAEPLF010000000
VERSION     DAEPLF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7898
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.160"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..149
                     /locus_tag="KR314_002790"
     CDS             <1..149
                     /locus_tag="KR314_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBH0329160.1"
                     /translation="SKSMILNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKE
                     KLNI"
     gene            406..1299
                     /locus_tag="KR314_002791"
     CDS             406..1299
                     /locus_tag="KR314_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0329161.1"
                     /translation="MQLYNTPFNTNKHLIYFFTSTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            1477..2301
                     /locus_tag="KR314_002792"
     CDS             1477..2301
                     /locus_tag="KR314_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0329162.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKHEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREVVPDYQSEGILWEKLSKFIKEEHIE
                     VSRQPNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            2400..3008
                     /locus_tag="KR314_002793"
     CDS             2400..3008
                     /locus_tag="KR314_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0329163.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            3112..3288
                     /locus_tag="KR314_002794"
     CDS             3112..3288
                     /locus_tag="KR314_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329164.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            4584..5336
                     /locus_tag="KR314_002795"
     CDS             4584..5336
                     /locus_tag="KR314_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329165.1"
                     /translation="MLKVNQLCKSYKTGNKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            5326..7437
                     /locus_tag="KR314_002796"
     CDS             5326..7437
                     /locus_tag="KR314_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329166.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYI
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVAGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIERVYMKKSSYDMFFNSK
                     DFSKLIDKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         2970 a         1006 c         1023 g         2899 t
ORIGIN      
        1 tatctaaatc catgatttta aatatggact acataaaaag tataaaagta atatcacata
       61 atcgcatgca agctacttta aaaaacaacg aaaaagttat tataaatcgt cgttatataa
      121 aaaatattaa agaaaagcta aacatttaat aaaaatctaa agtaatttat taatatcact
      181 aattgcaaat ttattgacct atttaataat taataataat ataatatgtt caatattaaa
      241 tactaattaa ttatatttag tgtaaaaata attaatcaaa atataaaaaa taggatatta
      301 ttataggcaa taactaacat caaacgataa cttaatacat ataaaaataa ataaacaatt
      361 aataaaaatt aaaacttcat tataaaacgg aaggatagtg aaaaaatgca actatataac
      421 acccccttta atacaaacaa acacttaatc tatttcttta catctacgct agtattgaac
      481 tggatattca gtcttacacc tgcgttgctc ggaattacaa atacaatact tggagaaatc
      541 attttttatc ttgcatatac atcaccagct attgtaggtc tattttttat ttctacaatc
      601 tatccacaaa gtgcaaaaca agattttatt tatagatacc taagctttaa agaaattaga
      661 ataaaatgga tactattaac aatattattt tttgctttaa cttttattac ttcgttaata
      721 atcggaaatt actttaatgt aaaaacttct gatatagatt ggatacacat agcagtgttt
      781 agaccttcta aaatagctta tatgctatta gcaagtttta tattgagtat acttagtcaa
      841 gaagctggat ggagaggata cgccattgac aaattattag ttagatttgg atttataggc
      901 tcttcaatta ttttagggct tgtttgtgga atctggtatc tagggtcgta ttttacacct
      961 aatcaaatac catataattt ggcacaatac tccctatttg atgctttttt atttatacca
     1021 aacattgtac ttttaaactt tataattaat tttgtatata tcaatacaag tagaagcatt
     1081 ttatcagctg ggttagttca tatgatgtac tattttttca acattcaatt acttctacac
     1141 taccctatta aattatgtgt aatagctcaa tatgtacaaa taatatttgg tttaatattt
     1201 cttgtatata tgatttcttc taataaattt aaacagaagg taaattctga aattgaacag
     1261 ataaagtcgg atgattttga atttgagtta gactggtaat tttaatatga attataaatt
     1321 aagataatct atgatttgat acgattacta cttaaatata atttactctc tataatttat
     1381 tgcaacatct ggaaaataat tgtatttcca caatcgcttg aagtatataa aaacaaaata
     1441 tatactaatt ataagtgaaa aggaggtcat attttcatgt ttaaaatagg agaggtttca
     1501 aaactaacac aaatttctat tcgtatgttg cgttattatg atgaacttgg tattttaaag
     1561 cctgcagaaa tagacaaata tacaggtcat agattatact cagttgaaca gatttcaata
     1621 ttacaaagaa ttgtattatt gagagattca aaatttagcg ttgcagaaat agcaaatatc
     1681 gtacataatt ggaatgatga atttgtaatt aaagaattga atagaaaaaa gaatgaaata
     1741 caaaaggaaa taaagcacga acaacaaaga ataaataaaa ttgataaatt tatagaagct
     1801 ataaactgtg ataaagatga aattcattac aatgttgtct ttaaaaagat tccaagttat
     1861 aaaatcatat cacttagaga ggtagtccca gactaccaat ctgaaggtat actctgggaa
     1921 aagttatcta aatttattaa agaagaacat atagaagtat cacgtcaacc caacaataat
     1981 atagcttttt accatgatga agaagtcaaa gataatggtg tagatattga agttggaatg
     2041 gttgtaaaga agattggaaa gaacaaatct ggatttatat atcgtgagac agaagaaatt
     2101 gatatgatgg catgtactat ggtttatggt ccatatgaaa atatagctgg tgcatatgaa
     2161 tcgttctgtt attggttaga taaaaatagt gattatcaaa tatcaggtat aaatagacaa
     2221 atcggtcata agggtgagca taatgaaata aatcctgaaa attatcttac agaaattcaa
     2281 atccccctta taaaagtcta atcgattagt acttaaatac ttaagtacta atcacccatt
     2341 gacatcccaa aattttcatg atagtatcta aacgtgtatt aaacatgttt aattttttta
     2401 tggagaaact tttaagggga aaaattatga aagcagattc aacaaaggaa caattaatta
     2461 atgccacaat ccagttacta tcttcacata gagatgtttc aagcattacg gctagagaaa
     2521 ttgtagctaa agcaaatgtc aatttagcta tgattaatta ttattttaaa tctaaagagg
     2581 agttaattaa tatttctatt aaaaaatttt taaagcgtca tatggatgaa tataaggcta
     2641 cacatgatac taaaaattta gcacctaaac agcaattaag ggaaatacta attgcatttt
     2701 ttgattttat aggtgaacat agccaattta taaagatttc tgtaccctat atattgatac
     2761 aagaagaaat tgtttatcca tttgagatac tacctttaat taaggctcac tatgaaaatc
     2821 agaataacaa aaaaactgaa gctgaataca gaactattgc gtatcaaata acttcattta
     2881 ttcagttgct ttttcttcgt tctaatgctt ttttcaagta tagtggtatt gatattctta
     2941 atgttgaaca gagaaatggt ttaatagatt ttcaattaga tttattattg gtagatatta
     3001 caaaataata ctatattaat tttttatgta cattttttta ttttatggta acgtttgaaa
     3061 atattctcat tgactaaaaa tacttgcatt aaataagggg gaggaatttt aatgtttttt
     3121 acatttatag gagtaatatc agcaatttta ttgttgtgta caattatatg tggtttatgg
     3181 ataaaactta ataatcgtaa taatgactct aattctcact ttactttttc aatactttca
     3241 attgcttttt cactgttaat tgtggtggcc tttttagtaa gaaattaatg tctttgttat
     3301 gctggggttt attattatag atttttatta ttgcatgatt tttactatat tatagtattt
     3361 ttattatatt acagggtctt atttattata gtgttttgtt attacagcat tttattatat
     3421 tacagagtcc tatttattat aggattttta ttatattgta aaatcttatt tattatagga
     3481 ttttgttata ttacagactt ttattatatt acaatctctt atttattgca gtgttttgtt
     3541 attacagtat ttctattata ttgcaaactc ttatttatta tggtgttttg ttattgcaac
     3601 atttctatta tattataaag tcttatttat tacagttctt tgttatacta cagactttta
     3661 ttattacagc atttctatta caatctctta tttattacag ttctttgcta tactacagac
     3721 ctttattatt gcaacatttc tattacattg caaagtctta tttattacag ttctttatta
     3781 tactacagac ttttattatt acaacatttc tattatatta caatctctta tttattacag
     3841 ttctttatta tactacagac ttttattatt acagcatttc tattacattg caaagtctta
     3901 tttattacag ttctttatta tactacaaac ctttattatt acaacatttt attattttaa
     3961 cgtctttact ataatatagt aatattttaa tatctcaaaa tcatatcaat cacttttcag
     4021 catagtctaa aatacttcca ctttctaaaa ttatctatca tacctatact aataatactt
     4081 tttaaccttt ttattataca tttcaattgt ttttaacgac ctatttattt ccaaaactcc
     4141 aatatatttt ctcaattaca ttgtgttaat aacccctaaa tcctttcaaa ttatttacaa
     4201 tacattccat taaataatct tactgctcta taattctttt tttcttttta accctactat
     4261 acattattta catcattttt ttcaatacaa cattcgttat tctataacct ttttactttt
     4321 actatatacc ttttttctaa ctacaccttc aatgttccac atcatattac tttagcacaa
     4381 caattaatac ctaaaaataa ccctctaaat cctccaataa tataatcaat ttaactataa
     4441 taataacctc tcacactatc caaacaatat attttcaaac attacaaaaa aacatccaac
     4501 tattacaata ttgctcatat ccctcctccc ctttcaaatg ctatcattga cttacaagat
     4561 aaatgaaagt gaggatgata tatatgctta aagtaaacca attatgtaaa tcatataaaa
     4621 caggcaataa aacatatcct gtattaaaaa atatttcttt tgaaatcaac aaaggagagt
     4681 ttgtcgcaat catgggttca tcaggaagtg gaaaaactac tcttttaaat tgtatttcat
     4741 gttttatccc aattgataat ggggaaataa ttcttggaaa taataatata agaaatttaa
     4801 aagaaaagga actttcagaa gttagaaacc aacaactagg atttgtattc caagatttca
     4861 tgctatttga tggtcttagt gtctttgaaa atatatgtgt tccacaaatt atcaaaggta
     4921 gtcaagtctt acaaatggaa aaccgtgcaa ttaatctatg cgagctattt ggtattgaac
     4981 atatcagaaa aaaataccct gccgaaatat ctggtggaga aaaacagcgt acagcagtag
     5041 caagagcact catgaacaac ccagatttaa tattagcaga tgaaccaact ggtaaccttg
     5101 atagtaagtc ttgtcgtaca gtcattgaat catttttaaa agcaaagcaa gaattagatg
     5161 ctactatact catggttaca cacgatagct ttgcagcaag cttctgcgac cgagttattg
     5221 tgcttaaaga tggaataatt tataaagaag ttgtaaaatc aaattcaaga cataaattcc
     5281 tagatgaatt actagaagtt ttaaaagaaa tgggaggcgc tcaagatgac aattaataaa
     5341 ttatacagca aacttcgagc ctataattgg aagaactata caactttatt cttttgtata
     5401 atactttctg tggtattagt aacttcatat gctctaattt attttagccc aacaatacaa
     5461 aacatacttc caactggtgg agatagcaga aaacaggcta caatgatatt ctcagttgct
     5521 atattaggat gcgctgtctt tactacatac gcttcttccc tctttttaaa atttaaaagc
     5581 aaggaaattg gtattttcat ggctctagga acagataaga agaaattaaa aaaacttcta
     5641 ttttcagaga tattgttaat ttcaatagta tgctgttttt taggtcttat attaagtatt
     5701 cctgtatctt ttggtatatg gaaaatattt caattagtga ttattgatac cagagaaatg
     5761 acatatcatt ttggaactca aggattcata tatggattta tatttagtgt atttgtgaca
     5821 ttatgcatat ttatactttc tgtaaaattt attaatcgta caaatataat tgatatatta
     5881 aatgaccaga gaatatctga accaatacag gatgtaaagg actggtatgg tatattaggt
     5941 ataattttta ttatactagg tatttttcta ggatatatcg taccttcact aactataaac
     6001 aaattaaatt atagactacc atctgtatgg agctttactt atctactctc tcttattgga
     6061 atatatatga ttatgacata catagttgta catgctaaaa gaggaaaaca tccagaaaaa
     6121 tactataaaa acatcatatc aactagcatg atgagattta tgggaagaca aacagtaaaa
     6181 aacatgtgtg ttatttcatt tttgattgca ggagcattat ttgctgtatt ctatgttcct
     6241 aatatggttg caggtatcta tgatactctt aaaaataacc ctatagatta tacttttacc
     6301 tacaatcaaa gagataaaca ggtatcaaaa aaagatattg aacaattagc agataaacac
     6361 aatataaaaa ttcaaaacta tatggaaatt ccttctatag aattaattgt tgatggtgta
     6421 gaatcaatat atcataaaga tggaactatc gaaagggtat atatgaaaaa aagctcctat
     6481 gatatgttct ttaattctaa agatttttcc aagctaattg acaaaccaat aactataaaa
     6541 aatggtgaat atcttgctgt tgtaaataaa ggaaatgaaa atcaagatta taaatatacc
     6601 aaatttatat caagcccagt cacagaaaaa gaaatcccct ttagatatgc tggaaaaata
     6661 atatttaatg gttctttttt tcaaaataga agtatgaacg cctatgtcct taatgatgag
     6721 gattataaat catttactaa agatttatcc ataaaaaatt acaataattc tatactattt
     6781 aatgtagata aagataccta tgcttttgcc aatgacttaa agaaagaaat tataaaaaga
     6841 acatctaagg aaatgggcat gttcagtggt tatgatgaat acgtaaaaaa acgttatgaa
     6901 tcaactggaa gaaaatattt tatggatgaa gaatatccaa gtggtgaagg tcatttaaaa
     6961 ctagacccaa acaacaatca gttgtatctt aattggaaat actatcctaa ctttaaaatt
     7021 ttacaaagcc aagatatgat aaaaaacatg gcagtcttct taatgttatt catatatgtg
     7081 gcaatcatat gttttacagc agtaggaatc attgcatata caaggggtgt tactatagct
     7141 ataaatcaca agcaggtctt tatggattta aatcgattag gagcaaacaa tgattatata
     7201 aagttctgta ttagaaatca attaaagaaa gtattttcat accctcttat tgcaggaagt
     7261 tttttaattt atctgtttaa ttttatgatt atgttaaata atgatggtag aatcgtcaat
     7321 agtgaggtca atgctctttt gataaactta ggttttatag ggatttgtgg agtttatatg
     7381 taccttatat atctattaac ttataagaaa ttcaaaaaaa ttattggtat tttataataa
     7441 ggaaggttgt tagattttta aagtaataaa atcattactt tttataataa gaaaagcatt
     7501 agcattttaa attaaaaaag gttattagat ttttatgata tgaaaagtca ttgtattttt
     7561 atagtaagat aattatttgc tttttataat atgaaaatcc attatatttt tatagtaaga
     7621 taattatttg ctttttataa tatgaaaagt cattgtattt ttatagtaag ataattattt
     7681 gctttttata gtattaaaag tcattgcatt tttatagtaa gataattatt tgctttttat
     7741 agtattaaaa gtcattgtat ttttatagta agataatcat ttgcttttta taatatgaaa
     7801 agtcattaca tttttataat aataaaagca ttggtatttt ataataagaa aggttattag
     7861 attttttaat aagaaaatca ttagcacttc agaataag
//
LOCUS       DAEPLF010000161         7878 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEPLF010000161 DAEPLF010000000
VERSION     DAEPLF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.161"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..1213)
                     /gene="hflX"
                     /locus_tag="KR314_002797"
     CDS             complement(<1..1213)
                     /gene="hflX"
                     /locus_tag="KR314_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBH0329167.1"
                     /translation="MTKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSITQN
                     AKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRTAL
                     ILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKLEL
                     DRRRIEEKITSLNRELYDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLVDI
                     FKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRSTL
                     EEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVLDR
                     ILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDCVRCKFLIPYDKGHVVSYFNENTS
                     IINTEYREDGAI"
     gene            complement(1938..3770)
                     /locus_tag="KR314_002798"
     CDS             complement(1938..3770)
                     /locus_tag="KR314_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329168.1"
                     /translation="MSRMGRGPMGKSMEAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(3763..6009)
                     /locus_tag="KR314_002799"
     CDS             complement(3763..6009)
                     /locus_tag="KR314_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329169.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(6077..6538)
                     /locus_tag="KR314_002800"
     CDS             complement(6077..6538)
                     /locus_tag="KR314_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0329170.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(6843..7409)
                     /locus_tag="KR314_002801"
     CDS             complement(6843..7409)
                     /locus_tag="KR314_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBH0329171.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         2556 a         1403 c          789 g         3130 t
ORIGIN      
        1 aaatagctcc atcttctctg tattctgtat ttataatgct cgtattttcg ttaaaataag
       61 aaactacatg acctttatca tatggaatta agaacttaca tcttacacaa tctttaaata
      121 tgctatcaca aatacatttt atcattttat ctatatttat atctttttta actgaaacaa
      181 atataccttc tttatcgata ctatctaaaa ttctatccaa tacttctaca tctattaaat
      241 caattttatt atacacatgt atcataggta taccatctgc acctatttgt tttaaagtat
      301 cttctgtcac tttaatatga cttttatagc ttggatttga aatatcaata acatgtaaca
      361 gtacgtctgc ctcacatgct tcttctaatg ttgacctaaa tgcttttact aagtcatgtg
      421 gtagattact tacaaaacca acagtatcag ataataaaaa ttctttgtta tttgcaagaa
      481 ctatatttct gacagaagtt tcaagtgtag caaataacat gtttttctca aacactttct
      541 tttcttcaga gtttttaaat atatctacta gtttattcat tattgaagat ttaccagcat
      601 ttgtataccc gactagagct acatttggaa gatttgattt tcttctcata cttctttgag
      661 tttctctttg gaattttaaa tcatacaact ccctatttaa agatgttatc ttttcttcta
      721 ttcttctacg gtcaagttct aattttttct caccagaacc tctattttta gtaccaactc
      781 caccactttg acgtcctaaa ttttcatttg aacctataag tcttggtaac atatatttta
      841 aacttgctac ttctacttga agtttagctt ctctagtttt tgccctttgt gcaaatatat
      901 caagtatcaa tgccgttcta tctataattt cacaattaag cttttcttca agatttttta
      961 gttgtgatgt agaaagttca ttgtaaaata taactatctc tatgttttct ttctttatta
     1021 agtttaatat ttcctcaact ttacctgtcc caatgtaaaa tgctctatta acctgctttg
     1081 cattttgagt tatactccct acaacatcaa tatcacatgc tgaacataag ttttcaagtt
     1141 caatcattag ttcattaaag tcctcagatt tattatttat gtttaatcct actgttattc
     1201 cttttttcgt catatccata ttctccttat ctgctatttt ttatttaatc tatagcacga
     1261 gattatggct ctataattaa attcatcaag aattttgctt tttatcttat ccttaaatga
     1321 cacttcttat ttactattat aataaccata atcgtgcatt attttctcaa tcttatataa
     1381 aaaatcactt tcttacacct cctcccaaat atattatgca gttttttcaa taaaaaaact
     1441 ccttacaaat ctaagtaata caaaacaagc tacttatatc ataagaagga ttaaaattct
     1501 aaatatatat taaaaataca ttagtttaat taacttatat gtaattatca ttttatattt
     1561 aaccctattc ttaaatttaa gtagttattt gcatgattaa taaaagtaat aaaaacatat
     1621 acttcaatat aatcatcaac tattaaaatt aaattctatt tataataaaa tttaaagaat
     1681 aaaattaaat atacattttg ataatcacct ccaacatttt ccttactaca tattataata
     1741 aaataaacac tttgtaaaga cataaaatta tgtggctatc tcaaaatata gataatcctc
     1801 tttttcattg ttaaaatgat aaaaatacac tttatttcat acacaaaaga gtgccttatg
     1861 aattaaaaag ttcattttaa ggcaccctca tttattttat cttattaatt acatgtctat
     1921 gctaagatag ttttttgtta cattgcttca tcttcttcaa attgagaatt gtaaagattg
     1981 gcatagaatc cgcctttctt tagaagttcc tcatgatttc cttgttcaac tatatctcct
     2041 tcattcatta caagaatcat atcagcatca cgtatagttg ataatctgtg ggctataaca
     2101 aaacttgttc taccttccat aagattatcc atagcttttt gaatcaatac ttctgttcta
     2161 gtatcgacag aacttgtagc ttcatctaat attaaaattt taggatcagc taaaatagca
     2221 cgagctatag tcaataattg tttttgacct tgagaaacat tacttgcttc ttcatttaat
     2281 ttcatcttat atccatctgg taacgtctta ataaatctat gaacatgtgc agatttagca
     2341 gcctctataa cttcttcatc tgtagcattc aacttaccat atcttatatt ttccataatt
     2401 gaaccactaa ataaccatgt atcttgaaga accataccaa acatttctct aagctcacta
     2461 cgattaaaat ctttaatatt atgaccatca atcagtattg aaccgctatt aacatcataa
     2521 aatctcatta acagtttaac tattgtagtt ttacctgctc ctgtaggtcc aacaatagca
     2581 actttttgac caggtttaac atttactgag aaatcattta ttatagtctt gtttgggtta
     2641 tatccaaagt tgacattttc aaaatcaatt ttaccttcta aaccatctat tgaaactgca
     2701 ttttcaacaa tttggacttc ttcttcctct tctaaaaact caaatactct ttcagaagct
     2761 gctgctgttg attgcaataa attggcaacc tgtgcaatct gagtcattgg ctgtgtaaag
     2821 tttcttacat attgtataaa tgactgtatg tcaccaacct caattgtttt cttaattgca
     2881 agccaacctc ctaagattga taccattaca tatcccaagt ttccaataaa catcataagt
     2941 ggttgcatta tacctgataa aaattgagat ttccacgctg aattatacag gctatcattt
     3001 agtttatcaa actcttcaat tacttcatct tcacgattaa atgctttcac tattgtttgg
     3061 ccactataag tttcctcaac ttgaccattt acatttccta aatattcttg ttgatatcta
     3121 aagtattttt gtgatttctt tattattctt gaaattacaa acattgatat tggtaatatt
     3181 aaaactgcca ctaatgtcat aattccactt atagataaca tcattatcaa aacacctatc
     3241 atagttgtta ctgatgttat aagctgtgtc atactttgat ttaaactttg atttaaagta
     3301 tctatatcat tcgtaattct cgataatatt tcaccatgcg tcttagtatc aaaatacttc
     3361 attggtaatc tatccaattt tgctgaaatc tcttttctta aattatagga tactttttga
     3421 gaaattccac tcattataaa tccttgtata aaagagaaca gtgcacttat caaataaagg
     3481 aaaagtaaaa aggttaagat ttttccaatt gcattaaaat ctatacctac gtttcctcca
     3541 gaaactttac tcactaaccc ttcaaatatc tttgtagttg cctttcctaa aatttttgga
     3601 cctactatag aaaacgatgc actaccaata gcaaatacaa ttactaaaat tattgatatt
     3661 ttaaatttag ataaatatgc aattagtttt cgcatagtcc ctttaaagtc atttgctttt
     3721 tgtcctgctt ccatagattt tcccatagga cctcttccca ttctactcat tagctagctc
     3781 ctcctttgaa agttgtgata aagctatttg ctcataaact tcgcaatttt ttagaagctc
     3841 tttatgtgtt cctattccaa ctatttctcc ctcatttaaa acaattatct gttctgcatt
     3901 taagatagta ctaattcttt gtgcaactat tagaactgta ctttcagcag tttttgattt
     3961 taaagcctta cgaagagctg catcagtttt taagtcaagt gctgaaaaac tatcatcaaa
     4021 tatataaact tctggatttt ttgctattgc tcttgcaata gaaagtcttt gtttttgacc
     4081 tccagataca tttgttccac cttgagaaat ttcagtttta aaagcttctg gtttagaatc
     4141 tataaattct gtagcttgag ctatttcagc tgctgttctc atttcttcat ctgttgcgtc
     4201 ttttctacca tattttaagt tggattctat agtaccagaa aacaatactc ccttttgagg
     4261 tacatatcca attttttccc ttaaatcatg ttgagaaaca ttttttatat taactccacc
     4321 aagtaatatt tctccttcag taacatcaaa gaaacgagga attaaattta taagagttga
     4381 cttaccacta ccagtacttc ctataaaggc agttgtttct cctggctttg ctgtaaaaga
     4441 tatatttgtt aatataggtt cttctgcgtc tggatacatg aacgaaacat ttttaaattc
     4501 aacaagaccc tttttatctt ccttaaactc ttgtgcctct gatttttctg ggtcttttat
     4561 gcttatatct gacactaata cttcatctat acgtgtagct gaaacggatg ctcttggaag
     4621 cataatagat accattgata tcattaagaa tgacattatt atttgcattg tatattgtat
     4681 aaatgccatc atatctccaa cttgcattgc tccagaatca acactgtatg cacctctcca
     4741 aactataaga actgtgataa ggttcattat aagcatcata gcaggcatca tacatgtcat
     4801 aactctattt acaaaaagat ttgttttagt taaattttta tttgctttat caaatctttc
     4861 ttcttcatgt ttttcagtac taaaagctct gataactgga ataccagtaa ttatttcacg
     4921 gcttactaag tttaacttat caacaagctt ttgaaccagt ttaaacttag gcataaccac
     4981 tgagaataac actacaatta atgataagat agcaactact gctactgcta taatccaggc
     5041 cattgaagta tttgtgttta aaacctttat cactccacct atagccatta taggtgcata
     5101 gaatactatt ctaagcaaca taaccatcaa catttgtatc tgttgaatat catttgtact
     5161 acgagtaatt aatgaagctg tagaaaattc attcatttca gtatttgaaa atgacataac
     5221 ttttctaaat actccacttc ttaaatttct acctacactt gctgctactc ttgctgctaa
     5281 aaatcctact gttattgtag ctaccatact aatcattgct ataccaagca ttttagcacc
     5341 agtaaataat atataattat tttgagtttt ttctgtatca atacctattt tctcatattc
     5401 tgaccttaca tatgaaacag agctttgtgt tatcatactc tctggcatac tttcaaagcc
     5461 tttgctcatc tctttagtca atgtatctaa ctgttccttt ggcattgctg ataacaattt
     5521 aaatatatca acatctccac tttgagtagc ttgtggtgga agtttactca ttaactgagc
     5581 tttcatagct tttacctctt tactatcgcc ctcaagccct gatactatta aaatagcttt
     5641 accaaaaata acatttaaat catctatagt gtctttgtct ttagtattta actcataaag
     5701 agattcatct tttagacctg ggtacttatc tttatactta acataatcct ttttattcag
     5761 caaagtataa ttatctagta ctttatcctt actactttta tccataaata aagtaatttt
     5821 atttaattca ctctctctaa tcacagatgg aacagagttt tctactccac cttgttgtat
     5881 acctacattt actatattgg atgtgtattc tggtaaagaa aggtcacata ccgcttgaat
     5941 taccaataga cagactatta ttactattga taatgccgat ttctttaaat atttaattaa
     6001 tttaaacata catttcttcc cttctgtaat attaattagt ctaataacat taaattgaaa
     6061 aacattattt taaactttaa aaatattttt catcttcatg cttatgacat cctgctttta
     6121 aattatctcg tacttgcatt agaagtcttc taagtactat tttttcttct tcactaaatc
     6181 ctaaaaaaaa ctctctttcc aattccttgt taaattcttc aactttatta catatttcta
     6241 aacctttgtc tgtcaaaaaa atcctagata ctctttggtc cttttcatct tgttttcttt
     6301 ccacaagtcc agctttttcc atcctcttta tcataactgt agttgtagat gcttttactt
     6361 tccttttatc tgctaattct ttttgacttt gcccatcttt catctttaaa gaaaaaagta
     6421 tttggggttg accatgatat aagcctaatt tatcaaaata aaaatgagtt cttataaaat
     6481 atattcgcat taattgcaag aaaacatcat ttaatttttc ttcagaattg ctattcatac
     6541 ttgccctcct tattttttat attacttagt cggctaagca ttattttact atactgcatg
     6601 cattttttca atatatttat aaaaaattca tataaatttc atttattaaa tatttttata
     6661 tcatatagtt ataattttaa taaataatag taagattttt aattagtcag ctacttatat
     6721 tttttattgt aaagcttaaa ataaaccttt tcatatattt tcaaataaaa aagttgtccc
     6781 aaaataaatt ttgaaacaac atcatattca acagtatctt gattatataa cattacaata
     6841 ctctacttat cttcatatcc atttacaaca acgtctaact tcccagtttc aggtgatata
     6901 attagaccat gtacaataat accatctgga attaaaggat gcccttttat taaatcaata
     6961 ctatctttta cagattcttc tgcagaatcg aatccatgta accattgttt tgtatcaatt
     7021 ccagcactac gaagtgtaag tattacatca ttagatattc ctctatctaa aattttacca
     7081 agtaacttat ctgtatcaac attacacata ccacatccat gatgccctac aaccataacc
     7141 tcatctacat caaattcgta aattgcaact aaaacacttc tcataatact tccaaaagga
     7201 tgcattatag tagctcctgc attttttatt agtttcacat ctccattttt caaattcata
     7261 gccttaggca aaagctcagt taatcttgta tccatacaag ataagactac tattttttta
     7321 tttggatgtt ttgatgtcac atattgttca tattctttgt tctctacaaa tgatttatta
     7381 tattctaata tctcttcaag ttttctcata tgtaatatca ccctctttaa aaattttttt
     7441 attattatat cactattaaa gtacaatata tacacatata atttaattta tatttattaa
     7501 attattttat aagattatta ctctactata aatcttgtat ataacttgta tttataagta
     7561 tttgtatatt tattttttat aatctaaact aatattatat attttcatta tttttttaat
     7621 tataattttt ttaaaatttt attctaaatg ccttacttat aattaatttt ttatttcacc
     7681 tatatataat tagatttaaa aatgtttacc ttaccaatat aatgattaaa ctcaaaagtc
     7741 ttattgaaaa attttctatt tacataaatt gttcaatata taaaatagaa aattttttta
     7801 attttcaaaa tatattctac atatctaata tgtaattaca ataaaaaatg ctataattaa
     7861 catattatta attcgttt
//
LOCUS       DAEPLF010000162         7871 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEPLF010000162 DAEPLF010000000
VERSION     DAEPLF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7871
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.162"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            111..1982
                     /locus_tag="KR314_002802"
     CDS             111..1982
                     /locus_tag="KR314_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HBH0329172.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTDTVTSKTIEIGIGDEKIDLTANPVDSTGTNLDPSTEGFRVNKIVK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISS
                     KDSETGKYKFTIKYTDASGKAIELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADVDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKADITDSQRNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            2089..2796
                     /locus_tag="KR314_002803"
     CDS             2089..2796
                     /locus_tag="KR314_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBH0329173.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELAINKIQYVHAP"
     gene            2829..4664
                     /locus_tag="KR314_002804"
     CDS             2829..4664
                     /locus_tag="KR314_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0329174.1"
                     /translation="MKISKKIVSLLTMTFLTVTLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWKKSENAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENISFERISGNSRYDTSLKLAEKLDREKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSENGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERSLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTRSKHDLIDSLAVGV
                     LASKNGSPILLAGNKLDSSQKDVLNTKIIDKVIQIGGLGNENVVEDVIDMQEETKYTV
                     ETIDELNVAIKKSDANDIIRFKPEKDKNITNSFKIETNKAITIEFDGIYKQSITIDMP
                     NGDIKNFGDIDNDLVINNIKNETLVNEGSIQGIDVYDENGCKIENEDSGEIWLITILK
                     NSKDVYILNRGEITKISNNSSSVTIKNSGNINTISGSEEPSISGKKPITNDTDKDEDK
                     KARGLYPKVESCIPALKDYVRITISTKPESSKYKIYYRVVDEKPSAKYVGEKISTKGW
                     SMVTISNQSFIERAKDGSYIEAVEIDTSNNEVSRWGKSSITDDGI"
     gene            complement(4712..5107)
                     /locus_tag="KR314_002805"
     CDS             complement(4712..5107)
                     /locus_tag="KR314_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBH0329175.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIRFVFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKIFTNIVVIVLNYLFSKMIIFKK"
     gene            5334..7745
                     /locus_tag="KR314_002806"
     CDS             5334..7745
                     /locus_tag="KR314_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBH0329176.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISIGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
BASE COUNT         3279 a          956 c         1391 g         2245 t
ORIGIN      
        1 ggttaattat tacaataagt ctcatttatt gaaataatat caaatatata ttataattgg
       61 taaataagga aaaaataata aaaatttgaa ttttttaggg ggaaaatacc atgaataaaa
      121 aaaatctttc tgtaattatg gctgctgcaa tgataagtac atcagtagct ccagtttttg
      181 ctgcagaaac tacacaggta aaaaaagaaa caataactaa gaaagaagct acagaactag
      241 tttcgaaagt tagagattta atgtctcaaa agtatactgg tggttctcaa gttggacaac
      301 caatatatga aataaaagtt ggcgagactt tatcaaaatt aaaaataata actaatatag
      361 atgaattaga gaaattagta aatgctttgg gagaaaataa agaacttatt gtaactataa
      421 cagataaagg gcatataaca aatagtgcaa atgaagtagt tgcagaagca actgaaaaat
      481 atgaaaattc agcagacctt tccgctgagg ctaattctat aacagaaaaa gctaaaactg
      541 aaactaatgg aatttataaa gttgcagatg taaaagcttc atatgatagt gctaaagata
      601 agttagttat aactttaaga gataaaacag acacagtaac ttctaaaact atagagatag
      661 gtattggtga tgaaaaaatt gatttaacag caaatccagt tgattcaacg ggaacaaact
      721 tagacccttc tacagaagga tttagagtaa ataaaatcgt taaactaggt gtagcaggag
      781 ctaaaaatat tgatgatgtc caattagctg aaataactat aaaaaatagt gacctaaata
      841 cagtttcacc acaagattta tatgatggat atagattaac tgttaaaggt aatatggtag
      901 caaatggaac atcaaagtca attagtgata tttcatcaaa agattcagaa acaggaaagt
      961 ataaatttac tattaagtat actgatgcat ctggaaaagc aatagagctt actgtagaaa
     1021 gtactaatga aaaagattta aaagatgcca aagctgcatt agaaggtaat tcaaaggtta
     1081 aattgatagc tggagatgat agatatgcaa ctgcagtggc tatagcaaaa caaacaaaat
     1141 atactgacaa tatagttata gttaattcaa ataaactagt tgatggatta gcagctacac
     1201 cacttgctca atctaaaaaa gcacctatat tattagcatc cgataatgaa ataccaaaag
     1261 taactttaga ttatataaaa gatataatta agaaaagccc atcagctaaa atatatatag
     1321 taggtggaga atcagcagta tcaaatacag ctaaaaagca attagaatca gtaactaaga
     1381 atgttgaaag actagctgga gatgatagac atatgacttc tgtagcagta gcaaaagcta
     1441 tggggtcttt taaagatgca tttgtagtag gtgcgaaagg ggaggctgat gctatgagta
     1501 tagctgccaa agctgctgaa cttaaggctc ctataatagt aaatggctgg aatgatcttt
     1561 cagcagacgc tatcaaattg atggatggaa aagagattgg tatagttggt ggttctaaca
     1621 atgtatctag tcaaattgaa aatcaacttg ctgatgttga taaagataga aaagttcaaa
     1681 gagttgaagg agaaacaaga cacgatacta atgctaaggt tatagaaaca tattatggaa
     1741 aattagataa actatatata gcaaaagatg gatatggaaa taatggtatg ctagtagatg
     1801 cattggcagc aggacctcta gcagcaggta aaggtccaat acttctagct aaagctgata
     1861 taacagactc acaaaggaat gcacttagta aaaaattaaa tcttggtgca gaagtaactc
     1921 aaataggtaa tggagttgaa ttgacagtaa tacaaaagat agctaaaata ctaggttggt
     1981 aataacttat actgtggaga agattcctta attaatacag gaatcttttt ctataattat
     2041 gctatttatt gtaaattata acattttata aagtaggagg atgtagtagt ggtagatgca
     2101 actttatttt ttacaccaca tcaagatgat gaaaccttaa gtatgggaag tgctataatt
     2161 gaacatgttg aaaaaagtga tacacatgtc atattatgca cagatggtag taaatctata
     2221 ataagaaaag ttttggatga tggagggtca tgttcttatc atattaaaga tgttcataaa
     2281 tactctttat cagaaagtga attttcaaaa gatagagatg aagaatttaa ggatagctgt
     2341 gaagcaatgg gtgtaaaaga aagtaatata catatagaag ataatagagc acatgatggt
     2401 gagttaagta aagaaaaggc aagggaaata atacttaagt acttagaaga atatccagat
     2461 gcaaaagtaa aaactgttac tccgtttaaa gctagtggaa tacatgaaga ccatagggca
     2521 ttaggagaag ctgcactaga gttatataaa gaaggcaaaa taaaagattt aagattttat
     2581 gtagaaccat acgactataa ggattttaaa aaagttaatc caaatgtaga ggtatggaag
     2641 gtattaccta gtcaagagga gaagttatta agtgcaatga atgcatataa aaaatggaat
     2701 cctgaaagtg gacattatgc tataggatat cattcagtaa aatctcattt tgatgagcta
     2761 gctataaata aaatacagta tgtacatgct ccataaaata ttatgaaaga attaggaggt
     2821 aagaattaat gaaaatatca aaaaagatag tgtctttgtt aactatgaca tttttaactg
     2881 ttacattata tggaaataca tctaatgcat ctacaaaaga tacattaaca ggttctggaa
     2941 gatgggaaac agcaataaaa ataagtcaag caggatggaa aaagtctgaa aacgctgtgc
     3001 tagtaaatga taattcaata gcagatgctt tatcagctac tccatttgct aaagcaaaag
     3061 atgcaccaat attattaact caaagtaata aattggatag tagaacaaaa gcagaattaa
     3121 aaagacttgg tgtgaaaaat gtatatttaa taggtggttc aattgcatta agttcagaga
     3181 ttgaaaagca gttaaatgca gaaaatataa gttttgaaag aatatctgga aatagtagat
     3241 atgatacttc tttaaaatta gctgaaaagc tagataggga aaaatctata tctaaaatag
     3301 tagtagtaaa tggagaaaag ggtcttgctg atgcagtgag tgttggagct atagctgctc
     3361 aagaaaacat gccaataata ctttctgatt cagagaatgg aactgaagta gctgataatt
     3421 ttatagatag taaagatata gaaaaatcgt atgtaatagg tggtacatat tctatttcta
     3481 attctgtaga aagaagttta ccaaatgcaa caagaatagc aggtagtagt agaagtgaga
     3541 caaatgcaaa aattatagaa gaattttaca aagatactga tataaaaaat atttatgtaa
     3601 caaaggatgg tacaaggagt aagcatgatt taatagattc tttagctgtg ggtgtattag
     3661 cttctaaaaa tggttctcca atacttttag caggaaataa gctagatagt tctcaaaaag
     3721 atgtgttaaa cactaaaatt atagataaag ttattcaaat tggcggattg ggtaatgaaa
     3781 atgttgtaga agatgtaata gatatgcaag aagaaactaa gtatactgtt gaaactattg
     3841 atgaacttaa tgttgctata aaaaaatcag atgcaaatga catcataaga tttaagccag
     3901 aaaaagataa aaatataact aattctttta aaatagaaac aaataaagct ataactattg
     3961 aatttgatgg tatatataaa caatcaataa ctatagatat gccaaatgga gatataaaaa
     4021 actttgggga catagataat gatctagtaa taaataatat aaaaaatgag actttagtta
     4081 acgaaggaag tatacaaggg atagatgttt atgatgagaa tggatgtaaa atagagaatg
     4141 aggattctgg agaaatatgg cttataacga tacttaaaaa ttcaaaagat gtatatattt
     4201 tgaatcgtgg agaaataact aaaatatcta ataattcttc tagtgtaaca ataaaaaatt
     4261 caggtaatat taatactata agtggaagtg aagaaccatc aataagtggt aaaaaaccta
     4321 taactaatga tacagataaa gatgaagata aaaaggcacg tggtttatat cctaaagtag
     4381 aatcttgtat acctgcttta aaagattatg taagaataac tatatcaaca aaaccagaga
     4441 gttcaaagta taaaatttat tatagagtag ttgatgaaaa accatctgcg aaatatgttg
     4501 gagaaaaaat tagtactaaa ggttggagta tggttacaat atcaaatcaa tcttttattg
     4561 aaagagccaa agatggatca tatatagaag cagtagaaat agatacctca aataatgaag
     4621 tttcacgatg gggaaaatct agcattacag atgatggaat ttagacatta aacttattca
     4681 ttttgcagat tactatttag taatctgcta tttatttttt aaaaattatc atcttgctaa
     4741 acaagtaatt taaaactata acaactatat tagtaaatat ttttacaata acatcattta
     4801 caaaaagcaa atcaatcatt acatacataa ttaacatttc aacgacacca gaaagtagcc
     4861 taaaactaac aaaagacaaa aattctttaa atacgaacct tatttcaatt cttttacttt
     4921 caaatacaaa aaacttatta gtaacataag caaataaaac agcaagtatc catgccaaag
     4981 catttgcaac cataaaatta aataaaataa ctctagtaaa aaataaatac gaaactatat
     5041 ttacaagagt agtaaatgct ccaaaaaata aatataatat tgtctctcta tgctttttta
     5101 aaatcaaata taatcctcct aatctaattt aatataaata tacttaaaga tactaacaaa
     5161 cattagtata gcacatctat taataaatca atatatctta aaatattata aaaataaaac
     5221 tataatattc tataattaat atgtactcat aatccatatt ttatcaaaat aaaatatgac
     5281 cttaatttag aaatctgata taataaaaat taaatacata taagggggta aacatgagaa
     5341 agtacaaatc aaaaaaattg tcaaagctac tggcattgtt gacagtttgc tttttaatag
     5401 tgtcaacaat acctgtctca gcagaaaacc ataaaactct agatggagta gaaactgcag
     5461 agtattctga aagttatctt caatacctag aagatgtcaa aaatggagac acagcaaaat
     5521 ataatggagt aatacctttt ccacatgaaa tggaaggtac aacacttcga aataagggca
     5581 gaagtagtct tccatcagca tataaatcaa gtgtagcata caacccaatg gatcttggtc
     5641 ttactacacc agcaaaaaat caaggaagtc ttaatacatg ctggtctttt tcaggtatgt
     5701 caactttaga agcatatctt aaattaaagg gatatggaac gtatgactta tcagaagaac
     5761 atttaagatg gtgggcaact ggtggaaaat atggatggaa cttagatgat atgtcaggta
     5821 gttcaaatgt aacagccata ggatatctga cagcatgggc aggtcctaag ttagaaaaag
     5881 atataccata taatcttaaa tctgaggcac aaggtgcaac taaaccttca aatatggata
     5941 ctgcacctac tcaatttaat gtaacagatg ttgttcgtct taataaagat aaggaaactg
     6001 taaaaaatgc tataatgcaa tatggttcag tgacatcagg ttatgcacat tattcgacat
     6061 attttaataa agatgaaaca gcatataact gtacaaataa gagggctcca ttaaatcacg
     6121 ctgtagcgat agtaggatgg gatgataatt attcaaaaga taactttgca tctgatgtaa
     6181 aaccagaatc aaatggagca tggttagtaa aaagcagttg gggagaattt aattctatga
     6241 aaggattctt ctggatttct tatgaagata aaactctact aacagataca gataactatg
     6301 caatgaaatc agtatcgaaa ccagatagtg ataaaaaaat gtaccaactt gaatatgctg
     6361 gtcttagtaa gataatgtca aataaagtaa cagcagcaaa tgtatttgat tttagcagag
     6421 attctgaaaa acttgactct gttatgtttg aaacagactc tgtaggagca aaatatgaag
     6481 tatattatgc accagtagta aatggagttc ctcaaaacaa ctcaatgaca aaacttgcaa
     6541 gtggaacagt atcatattct ggatacataa atgtacctac taattcttac agcttaccaa
     6601 aaggtaaagg agcaatagta gtagttatag acaacacagc aaatcctaat agagaaaaat
     6661 caactttagc atatgaaact gatatagatg gatactatct atatgaggct aaagcaaact
     6721 taggtgaaag ctacatactt caaaacaata agtttgaaga tataaataca tatagtgaat
     6781 tttctccttg taactttgtt ataaaagcta taacaaaaac atcttctgga caagctactt
     6841 caggagaatc tttaactgga gcagatagat atgaaacggc agttaaagtt agtcaaaaag
     6901 gatggacttc ttcacaaaat gcagtattag taaatggaga cgcaatagtg gatgctttaa
     6961 cagctacacc atttacagca gcaatcgact ctccaattct tttaacagga aaagataact
     7021 tagattcaaa aactaaggca gagttacaaa gattaggaac taaaaaagtt tatctaatag
     7081 gtggagaaaa ttctttaagc aagaatgtac aaactcaact tagtaatatg ggtatatcag
     7141 tagaaagaat ttcaggtagt gatagataca agactagtat atctctagct caaaagttaa
     7201 atagtataaa atctgtttca caagttgcag tggcaaatgg tgtaaatgga cttgcagatg
     7261 caataagtat tggtgcagca gctgctgata acaatatgcc aataatactt actaatgaaa
     7321 agagtgagtt gcaaggtgct gatgagtttt taaattcatc aaaaataact aagtcttata
     7381 taattggtgg tacagctact ttatcatcaa atttagaaag taagctttca aatccaacaa
     7441 gacttgcagg aagtaataga aatgaaacta atgctaagat aatagataaa ttctacccta
     7501 gctcagattt gaaatatgct tttgttgtta aagatggttc aaagagtcaa ggagacttga
     7561 tagatggact agcagtagga gcattaggtg ctaaaactga ttcaccagta gttctagttg
     7621 gaaataagtt agatgaaagt caaaaaaatg tacttaagtc taagaaaata gaaactccta
     7681 ttagagttgg tggaaatgga aatgaaagtg cttttaatga actaaatact cttttaggaa
     7741 aatagtgaat ggaaagatag taaggatata cattttatta ttaaaaaata ttcaatagaa
     7801 gtttataaat ataaaataac aaaaaggggt gtctcaaaat gaacttttta gttcatgagg
     7861 cactcttttt t
//
LOCUS       DAEPLF010000163         7756 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEPLF010000163 DAEPLF010000000
VERSION     DAEPLF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.163"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            150..1436
                     /locus_tag="KR314_002807"
     CDS             150..1436
                     /locus_tag="KR314_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBH0329177.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1548..2021
                     /locus_tag="KR314_002808"
     CDS             1548..2021
                     /locus_tag="KR314_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1680..1682,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBH0329178.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            2053..3363
                     /gene="grdB"
                     /locus_tag="KR314_002809"
     CDS             2053..3363
                     /gene="grdB"
                     /locus_tag="KR314_002809"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3100..3102,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBH0329179.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3620..5152
                     /locus_tag="KR314_002810"
     CDS             3620..5152
                     /locus_tag="KR314_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBH0329180.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNSDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5163..6308
                     /locus_tag="KR314_002811"
     CDS             5163..6308
                     /locus_tag="KR314_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBH0329181.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            6618..7697
                     /locus_tag="KR314_002812"
     CDS             6618..7697
                     /locus_tag="KR314_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0329182.1"
                     /translation="MSRVKNVVGLLGTKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
BASE COUNT         3001 a          896 c         1593 g         2266 t
ORIGIN      
        1 caataaatat tttttagtaa taaaataaag atttaaggca taaaaacaca ataatatatc
       61 tttaaaaaaa taagataaat tgtaaaatga tataattttg agaaaaactc aaatgcatat
      121 acacaaatta aataacagga ggtgcaagta tgcgtcttga attagggaaa atctttataa
      181 aagatattca atttggtgat gtaacagaag taaaagatgg agtattattc atcaataagc
      241 aagagatgct acaagaaatt ggtggagatg agcatattaa atctatagat atagaattag
      301 ctcgtccagg tgaaagcgta agaataacac ctgtaaaaga tgttattgag ccaagagtaa
      361 aagttgaagg taatggggga atcttccctg gtatcatgtc taaagttgat actgtaggag
      421 aaggaaaaac tcatgcttta aaaggtgtag ctgttgtaac tactggaaaa atagtaggtt
      481 tccaagaagg tataatagac atgactggag aaggagctaa gtatactcca ttttctaaat
      541 taaataatgt agtagtaata gctgaaccta ttgatggttt aaaacaatac gctcatgaaa
      601 aagctgttag aatgatagga ttcaaagcag caatgtactt aggagaagta gcaagaaatc
      661 ttactccaga tgaagtttca gtatatgaaa ctaaacctct tcttgaatca ataaaagaat
      721 atccagagct tccaaaagta ggatatgttt acatgcttca gactcaaggt ttattacatg
      781 atacttatgt atatggagta gatgcaaaac aaatagtacc aactttatta tatccaacag
      841 aattaatgga cggagcaatt gtaagtggta actgtgtttc tgcatgtgac aaaaacccaa
      901 gttatgttca tataaacaat ggggttgttg aagacttata tgcacgacat ggtaaagata
      961 taaactttgt aggtgtaatt ataactaatg aaaatgttta cctagcagat aaagaaagat
     1021 cttcaaactg gacttctaaa ttatgtaaat atttaggatt agatgcagtt atagtttcac
     1081 aagaaggttt tggaaatcct gatactgacc ttataatgaa ctgtaagaag atagaaatgc
     1141 aaggtgttaa aacagttata gttacagatg aatatgctgg acgtgatgga ggttctcaat
     1201 cacttgcaga tgcagatgta agagctgatg ctgttgtaac aggtggaaat gccaaccaag
     1261 ttgtagttct tcctaagtta gataaagtta ttggacattt agaagtagta gatgtaatag
     1321 ctggtggttc tgatggaagt ttaagagctg atggaactat tgaagttgaa atccaagcta
     1381 taacaggtgc aactaatgaa acaggatttg gtcatttaac tgctaaagga tattaagaag
     1441 tattttatta aacgattgag taatgttaat aaaacatatt atagataatt ataataagtg
     1501 ttatagatat ataaaaatat attagaaata taataaggag gattattatg agtttactta
     1561 gtaataaaaa ggttcttata ataggtgacc gtgatggtat accaggacct gcgatagaag
     1621 aatgtgtaaa aacagtagaa ggagcagagg ttgttttctc atctacagaa tgctttgtct
     1681 gaacagctgc tggggctatg gacttagaaa atcaaaacag agttaaagat gctgctgata
     1741 aattcggagc tgaaaatgtt gtgattttac taggtgctgc tgaagccgaa gctgcaggtc
     1801 ttgcagccga aacagtaact gctggagatc caactttcgc tggaccactt gctggagttg
     1861 ccttaggatt aagtgtttac cacgttgttg aggaaccaat aaaatcatta tttgatgaaa
     1921 gtgtatatga agaccaaata agtatgatgg aaatggtttt agaagttgaa gaaatagaag
     1981 aagaaatgtc tggtataaga gaagaatttt gtaaatttta atgcctaaaa gcaaaagggg
     2041 tgaaactaag agatgggaaa actaaaagca gtacattata ttaatcagtt ctttgctggt
     2101 ataggtggag aagaaaaagc tgatactaag ccacatgtgg cagaaacttt acctccaata
     2161 agtttacaac ttgataaatt attaggtgaa gatattgaaa tagttggaac agtagtttgt
     2221 ggagatagct atttcaatga aaatattgat agtgcaagtg aagaagtttt atcaatggta
     2281 aaaggttttg aaccacaact tttcatagct ggtcctgcat ttaatgctgg tagatatggg
     2341 gttgctgctg gaacaataac taaagttgta aaagatgctt tgaatatacc agctttaaca
     2401 ggtatgtatg ttgaaaatcc aggagcagat atgttcaaaa aggatgttta tgtagtagaa
     2461 acttctgatt ctgcagcagg tatgagaaag gcacttccta agatagctaa acttgcagta
     2521 aaacttgcta atggagaaga aattggaact cctaaagatg aaggatacat agctagagga
     2581 ataagagtta actacttcca tgaagacaga ggttctaaga gagctgttga catgttagtt
     2641 aagaaaataa aaggtgaacc atttgaaact gaatacccaa tgccaaactt tgatagagtt
     2701 gacccaagta aagctgtaaa agatttatca aaatgtaaaa tagctttagt tacttctggt
     2761 ggtatagttc caaaaggaaa tccagataga atagagtctt cttcagcttc taaatatgga
     2821 acttattcta tagcaggagt tatggattta acagaagaga cttatgaaac tgcacatggt
     2881 ggatatgacc cagtgtatgc aaatttagat gcagatagag tattacctgt tgatgttctt
     2941 agagatttag aaaaagaagg tgtaattgga aaattacatg agactttcta tacaactgta
     3001 ggtaatggta catctgttgc aaactctaaa aaatatgctt ctgaaattgg agcagcatta
     3061 gtggctgatg gtgtagatgc agtaatatta acttctacat gaggtacttg tactcgttgc
     3121 ggtgcaacga tggtaaaaga aatagaaaaa actggacttc cagtagttca tatgtgtaca
     3181 gtagtaccta tttcattaac agtaggggct aatagaatag ttccaactat agcaatacct
     3241 catccacttg gaaatccagc gcttgaccct acagaagaaa aagccctaag aagaggcctt
     3301 gtagaaaaag cattaaatgc tttaactaca gaagtagatg gtcaaacagt atttgaaaaa
     3361 taaaaataac acacacatat attatataga taaaaatata aacacacact aaaaataaaa
     3421 aataactata cacaaaaaaa ataatataga tttcatgaat ggataaattg gagttattca
     3481 ttcatgaaat cttaaaaaat gccaaaatac ataaacaata atagaatcaa atatacaata
     3541 aaatacaatg attagagctt ttaataagtt taataagatt aaaaaataag tgttcataaa
     3601 atttatggag gtgcacaaaa tgacttatcc agttttaaaa ggggcaggct atgtacttat
     3661 tcatactcca gatatgatag tgcaaaatgg aagtacttgt acagttgaaa gagcaacaaa
     3721 ttcagattca gaatttttaa aagaagtaag taatcatata agaagttatg aagatgtagt
     3781 taactacatg ccaaatcaag tttatatagg aaatagaaga ccagaagaat taagagattt
     3841 accaatgcca tggtgtgaac aaaagattga gggaactaga aatggtaagt ttggagaaat
     3901 aatgcctcaa gatgagttca tagctttaat gcaaataagt gatgcatttg atttagtaaa
     3961 attatcacaa gaatttattg atgaagtaaa gccaaaaatc gaaaataact accctgaaat
     4021 tgcaccattt gtaggtaaat taaagggtga tgatatagaa gaaggtaaag aattagtagc
     4081 aacacacata gctgaaggat tataccatga tggaaaattt gttggttatg taaaaagagc
     4141 acatgatgta gatgttaact taaatgccca tacaatgttt gaaaatctag tagtaaaagc
     4201 atctggagtt ttatctgcta ttcaaatgct aagacatagc aaaatagacc ctgctgaaat
     4261 agattatgtt attgagtgtt cagaagaagc ttgtggagat ataaaccaaa gaggtggagg
     4321 aaactttgct aaatcaatag ctgagatagc aggacttcag aatgcaactg gttctgacac
     4381 aagaggattt tgtgcagcac caactcatgc attaattcaa gcagcagctt tagtaaaagc
     4441 tggcatacat aaaaatgtaa tggtagtagc aggtggagca agtgctaaac ttggaatgaa
     4501 tgctaaagac catgttaaaa aaggtcttcc tgtacttgaa gatgttgttg gaggatttgc
     4561 agtacttgta tctgaaaatg atggtgtaaa tccagttata agaactgatt taacaggtaa
     4621 gcatactgtt ggaactggtt cttcaccaca agcagttatg acagcactta taacttcagg
     4681 tcttgataga gctaatttaa aaataacaga tgttgatgtg tattcagttg aaatgcaaaa
     4741 cccagatata actaagccag caggagcagg agatgttcca gaagcaaact acaaaatgat
     4801 aggggcactt gctgttaaac gtggagactt agaaaagaaa gaattgaaag attttgtttc
     4861 taacaaagga ttaccaggat gggcaccaac tcaaggacat ataccatcag gagctcctta
     4921 tataggattt ttaatagatg atttaactac aggaaataga aatagagcta tgatagttgg
     4981 aaaaggtagt ttattcttag gaagaatgac taacttattt gatggagtat cttttatagc
     5041 tgaaagaaat acaggtgtta ctgaagagac ttcaggaata tctaaggatg aaattaagaa
     5101 gataatagct gaatctatga aaaaactagc attagatatg ttagaagaat aggggtgagg
     5161 atatgtcaaa aaaagtaatc gcagatgtat tcttagaagt agctaatgca atagaaagcg
     5221 gcgaatttgg caaaaaagta aaaattggtg ttactacact tggaagtgaa catggtgtag
     5281 aaaacatggt taatggtgca caattagcaa aatctaattt atttgatatt gtgcttattg
     5341 gaccaaaagt ggaaactgac cttgaagtag ttgaagttaa tgatgaaaaa gaaatgcatg
     5401 caaaaatgga agaattatta gattcaggat atattgatgc ttgtgttact atgcattata
     5461 attttccaat aggtgtatct acagtaggta gagtaataac tccagcaaaa ggaaaagaaa
     5521 tgatacttgc aacaacaaca ggaacaagtg ctactaatag aatagaggca atggtaagaa
     5581 atgccattta tggtattgct acagctaaat caatgggtaa caaatgccca aaagttggaa
     5641 tacttaatgt tgatggagca agacaagttg agaagtgctt aaaagaatta aaagataatg
     5701 gatatgacat ggagtttgcg gattcaatta gagcagatgg tggatgtgta atgagaggta
     5761 atgatttact tgtaggtgca cctgatgtta tggttactga tacattatct ggaaatatat
     5821 ttatgaaagt attttcttca tatactacag gtggagatta tgaagctcaa ggctttggat
     5881 acggacctgg agttggagaa gactatgata gaaaggtact tatagtatca agagcatcag
     5941 gttctccggt agtagcaaat gctttaaaat acgcttatga tgttgtaaag ggagatataa
     6001 gtaatgttgc aagaaatgaa tttgcaaagg ttaaaaaagc aaaatttgat gacattatat
     6061 cctctcttac taaaaaagaa gttaaagctg aaaaagtaga agttaaaatg ccagataaag
     6121 aaattgttac tcgtcaaata gctggtgttg atataatgga tttagaagat gcagtatctg
     6181 agttatggaa aaacggaatc tatgctgaaa gtggtatggg ttgtacaggt ccaatagttt
     6241 tagtaaatga agctaaagga gacttagcag ttgaaacttt agttaaagct ggatatactg
     6301 caaaataagt acatttttta tatgtaaata agaacatata gttataattt tattgattat
     6361 aattatatgt tctttttatt ttatatcttg tcaatattta tatattaaca caatccattt
     6421 atttgattct ctagctaatt tattatattt attcaattaa aattaagctt tctaactaaa
     6481 taaaaactaa tgaagtatta aaatgaaatt aataaaatat gaaaaaattt taggaataaa
     6541 gtatctgtta gataaaaata agattaatta gtgatataat ataattaaga taaaatttat
     6601 tatttggagg taaaatcatg agcagagtaa aaaatgtagt aggattgttg ggaactaaag
     6661 gtgttgatgc actatatcta acaaagaaaa ctaatgtaaa ctatataagt ggttttcctg
     6721 atgaagaagc atatgcagtg atttgcaaag atggaaactt tttagttact gatagtagat
     6781 atatggagct tgcagaaaag gtttgcaaag attttgaaat tataaattgg cataattttg
     6841 atagaagtgt ggcaaaggca gttaaaagtg tatgtgataa agtgggtata aagaaacttg
     6901 ggtttgaaag aactaatata gtgtttgata aatatgaaga acttaaaaat ctgattgaga
     6961 aggataatgg agaattaata cctacagaaa atattgtaga gactttgaga tatgtaaaag
     7021 acaaagatga aatcaaaaat acaagaaaag cttgtgaaat agcggataag gcattagaag
     7081 aattgattcc acatataaaa gctggagtta gcgaaattga actggctaca aagttagagt
     7141 actttatgaa aatgaacgga gcacaaaata tagggtttga aactatactg atatcaggag
     7201 caaagacttc attgcttcat ggtaagccta gtgataagat tatagaaaaa ggtgattttg
     7261 tacttataga ctatggtgct atgtataatg gctatatatc tgatacaact agaactttta
     7321 tagttggagg agcttctgaa aagcaattag aaatatataa tttagttaag gaagctcaaa
     7381 atgtaggggt tgagaacatg aaagctggtg tacatgcgac aattccagat gctgagataa
     7441 gaaaagttgt caaaaaatat gaagactatt actatcaagg tataggacat ggagtcggta
     7501 gggatgttca tgaagaacct ttcattggta attatggtga taaaatcata gaagagggat
     7561 gtataataac aatggaacca ggtatttatt tccctggatg gggtggagtt agaatagaag
     7621 atactgtttt gattactaaa aatggtccag aaagacttac taaattccct aaagatttga
     7681 tgattttaga caaataattt aatttatata tattttttta cataaaatat aacataatct
     7741 cactattaag ctctat
//
LOCUS       DAEPLF010000164         7518 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEPLF010000164 DAEPLF010000000
VERSION     DAEPLF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.164"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(103..702)
                     /locus_tag="KR314_002813"
     CDS             complement(103..702)
                     /locus_tag="KR314_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329183.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(903..1115)
                     /locus_tag="KR314_002814"
     CDS             complement(903..1115)
                     /locus_tag="KR314_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBH0329184.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDISIEKLKEVIAELGFELKGIE"
     gene            complement(1188..2903)
                     /locus_tag="KR314_002815"
     CDS             complement(1188..2903)
                     /locus_tag="KR314_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0329185.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEEARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANINSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEKKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(2903..3457)
                     /locus_tag="KR314_002816"
     CDS             complement(2903..3457)
                     /locus_tag="KR314_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBH0329186.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(3450..4052)
                     /locus_tag="KR314_002817"
     CDS             complement(3450..4052)
                     /locus_tag="KR314_002817"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBH0329187.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNISIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNSLTHPRIREKIISEIEELR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSKQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(4061..6709)
                     /gene="polA"
                     /locus_tag="KR314_002818"
     CDS             complement(4061..6709)
                     /gene="polA"
                     /locus_tag="KR314_002818"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBH0329188.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINR
                     LEIDGYEADDIIGTVSKKAENNGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPAQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMVFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDTEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETIPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            7028..7110
                     /locus_tag="KR314_002819"
     tRNA            7028..7110
                     /locus_tag="KR314_002819"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7062..7064,aa:Leu,seq:caa)
BASE COUNT         2252 a         1323 c          746 g         3197 t
ORIGIN      
        1 aagaatatgt caaaaaaaga ataagctctt tcaaatgcac aaaaaattta tacccaagtt
       61 atacttaatc atgcaaattg gcagaactta ttctttaaaa ctctaacttt taagtttttc
      121 tttgataata taatttatta ctttttttct aatatcttca gcattataat catcttctac
      181 ttctatatcc aaaagttttg ctattgacct catatcttct ctagtctttg acctatagct
      241 tgatattaat tcatctttta ttttctctgg ttctaaaaga tattcttcat ctcctctttt
      301 gaaaacaacg tatttttgag catatgaact tctattatag tatatatatc ttgatatttt
      361 tcttaaaatt tgattatatg aaagtcgctt gttaatatca agttttaagt actctgaaaa
      421 agcaaatagc tctttttttg atttaaataa tgcttctttc tttatagtcc ttgatattga
      481 agttacactt tcttgatatg cacacttttt taacattttt aaatatactc tattaagacc
      541 aatactttga ttgaattttt ttatattatt attaataatc ttatccaagt taccttcaaa
      601 gtattttttt gctccttttt gtgatacata actctcaagt tctttgtggg ccatatcaag
      661 tatcattttt tttaaatcct ctttatatcc cctagattcc atgatactcc ctcccttagc
      721 tatttatagt atattcaaaa agctcaagaa atgtgagtca ttacaaaatt tttttacttt
      781 ttatgtatat attaatttaa aaggggagat aatagtaatg taaaatcaaa catactcaat
      841 ctcccccatt tttagacctc tattactccc ccaatagagg ccttttttta gtttatcaat
      901 ttctattcaa ttccctttag ttcaaatcct aattctgcta tcacttcttt taatttttca
      961 atagatatat catctttcat atctacagat gcacacttat tttctaaatc cacatctaaa
     1021 acttcaatac catctatatc ttctgttaat gcagttttaa gatggtttac acaatgacca
     1081 caactcatac cttctactaa tagtttcttc ttcatttaat tacctcctta taccccataa
     1141 gggtattcta tcatatagtt tatacttttt aaatagaaat taatcattta atttgattca
     1201 ttcgatttta tatctaaatt agatattccc tcatatacat caaaatcatt tggagtaatt
     1261 ttacctttta atctactatt acttacaata tagttgtttc tcacagctaa acttataaat
     1321 gttgcattat cttttatata tgtctgtaca tctttgtatg ctttttttgt ttcaatttca
     1381 gacgtagaac ttgtaagtgc agacatatat ccatttagct tatcatcagt atatccacta
     1441 cttgctataa tactacttgc atctggtata attgatagtt tccatccaac taatgctaaa
     1501 tcataatttt tagaattaag agcattttcc atatccttat ctgacagctg atttactgta
     1561 ctatttattc ctatagcttt taagttttca tttattatgt ttgcagtttt tattctctca
     1621 ctattatctc tgtttactat tatctttaag ttcatttcag aaagactttt ctttacttcc
     1681 tctttttctc tatttttttt cttttcagct tcttttttga ttctatcttc ttctgctttt
     1741 ttagcttttg cttcttcttc tggcgtaagc ttctttgagt tctttttagc tgattttttc
     1801 tgatttttta ctttattgtc attttgattt actgagttta tatttgcata ttctacctta
     1861 gcaagacact ctatcgcctt atctttatta aactctaaat ccttcatttc actattatag
     1921 tattttgatt ttgaattaag tggaaaatta actggagtag catcatccat ataaccttca
     1981 ctaataattc tatctttatc tatagagtga gcaattgctt ttctaaaatt aacatcttta
     2041 aaaaatggat tattataatt aaataataca cactcataat ctcttccttg atattttgtt
     2101 atattaaact ctttttcttg aaacttgctt aaatcattta atgtaacatt ggttatatca
     2161 ctatcaagag ccataaccat agatgtcctt gcctcttcat taggcaccat acctactttt
     2221 atgttcttca tggtctttgg aacttcttca tagtaatctt tgtttacaga taaaaccatt
     2281 ccttctcttt ctgtatatga ttctatttta tatttcccat ttcctatcat attattattc
     2341 ttgtcattta catcctttga atctaaatga ttttgagata cgattggaaa aattaaacta
     2401 tcaattgaaa aagcatatct agctttaaat tttattgtaa aatctttatc tgatttaata
     2461 ttcacagaag ctatattttt agtaaattca ttatatggac tatctgcact tttttgaatt
     2521 aaaccaatag tatagtttac atcatttgaa gtaacatttt ttccatcatg ccactttgca
     2581 tcttttagct ttatatctat actcattcca tcctgagcta tattatactc cttaactaat
     2641 tgaggaatta cattgtagtt ctcatctata gtaaataaac tatcataaac taaattcatt
     2701 atataaccaa cagacttatc agtattagtt ataggattta tagtttttgg agttaccatt
     2761 gttaagttta tatacttagc atctatagaa ctttgacttt gtaaacttga atcactacta
     2821 ctttgttttg ttttagtatt actacaacca gctaccataa gagttatagc taaaacgact
     2881 gttaagactt ttattctctt cattagtttt ttcctttctt gccatatatc ttattataat
     2941 gttcgtaatt attttttact ttttctacat attttgaagt ttgcttaaaa ggtatatcat
     3001 gtagattttc gccatcttta ctatattcat tattttctaa ccattttttc acattacctg
     3061 aaccaccatt atatgcagct atcactaaat ctaatttacc aaattctttg tataacttac
     3121 ttaaatacca acagccaagt cttatattag tctctgggtc gaaaatatcc acatttttta
     3181 aatttagttc ctctgctccc caatctctag ttatatctaa tatttgcatt aatccttttg
     3241 cttccttttt tgaaacagca gaactattaa acttactttc ggctttaata acactgtaaa
     3301 ctatattttc atctaaatta aattcttttg aatacttttc tacatactct gaatattttt
     3361 taggatataa aaatttatgt attactttgc tttccattaa tagtgcccca aataagatta
     3421 taaaaataga tagaatcaat actttcttac tattcacgcc aattctcctt catgtactca
     3481 ataaatttat gtgctttact ttctaattca gttattgttc ccgaattatc tattatataa
     3541 tcaccatatt tggatttttc ttcctgactc atctgtgaat ttatcctact aagtgcttcc
     3601 tgcttactac aattatctct cttttgtatt ctactaattt gtacttcttg tttgcaagta
     3661 actactaata gcttgtcaac catatctaaa aaaccacttt ctaccaatat tgctgcatct
     3721 aatactacta tattttcgcc tttttttctc aattcttcta tttcacttat tattttttct
     3781 cttattcgcg gatgagtcaa gctatttaat tccttaagtt tttcttcatc gctaaaaact
     3841 attttcccaa gagcttttct atttagagtt ccatcatcat tttttatact ttgcccaaaa
     3901 tggacaaata ctttttccaa taacaactta tcctcaaata tcttcctgga tataatatca
     3961 gcatccacta tagaaatatt aagatttcta aatatatttg ataggctgct ttttccacag
     4021 cctattcctc ctgtaagtcc taatatcaac ataaaatcac ctactttgtc tcataccaag
     4081 aactacctac atttaaatct acatctaagt gaacatccat atttacagca ttttccattt
     4141 cttcttttac tattttctta actatatcta tttcatcatc aattgcttca actatcaact
     4201 cgtcgtgaac ttgtagtata agttttgatt tcaaattgtt ctcttccaat tttttatgaa
     4261 catttatcat agcaattttt attatatctg ctgcacttcc ttgtattggt gcattcattg
     4321 caaatctctt tcctctattt ctttccataa aattgcttga ctttatttct ggtatatatc
     4381 ttcttctgtt taaaattgtt acagcatatc catcctcact agccttttct actgttgtgt
     4441 ccataaactc ttttatttta tggtatttag caaaataact ttcgatatat tctttcgcct
     4501 ttggaacagg tatatttaaa tcatcagcta gaccaaagtc acttttacca tatataatac
     4561 caaagtttac agctttagct gcacctctca attcactagt aacatcttcc ataggaacat
     4621 taaaaacttg tgaagctgtt ttggtatgaa tatcttcatg atgtttaaat gcttctatca
     4681 tattttcatc ctgactcata tgtgcaagaa ctcttagctc tacttgtgag taatcggcat
     4741 caaccaactt acagttatca tctgcaataa acacttttct tatttttcta cccatttcca
     4801 tttttatagg tatattttga agatttggtt ctgtagatga aattcttcct gtagtcgtta
     4861 tagtttgatt aaatgatgaa tgtattctat tactttctgg atttattata tttaaaagtc
     4921 cttcaacgta agtagaattt aactttacta tttgtctata ctcagttatc ttatctatta
     4981 ttggatgctt atctcttaat ttttctaaaa catctgcatt agttgaatat ccagttttag
     5041 tttttttaat tattggaagc tctaattttt caaataatat aacacccaat tgttttggtg
     5101 aatttatatt gaatggctct cctgataatt cataaatttc cttttctagt gtagctatta
     5161 tttctttaaa ctctacactc aattcatcca atatactttt gtctactttt ataccttcat
     5221 actccatatg acctaagact tctactaaag gcatctctac atgataaaaa agaccatcca
     5281 tactcatatc cattagactt ctttccatca taggaattgt ttctttaact gtgcatatta
     5341 tttgacccat atactctgct aaatcctcaa attctaaatc ctcatagttt ttagatttaa
     5401 caccctttcc taataattct tcttttgatt taaccttttt tgataaatac ttcatagcta
     5461 ttgcactaca atcatatgag gtagatgaag ttgaatctat taagtactca gctattgtta
     5521 tatcaaaata catattttct aagtttatat tatatggttt taaagatata taatcatctt
     5581 ttaaattata tccatacttt ttaatttcat tatttgaaaa tatactatct aactttgaca
     5641 cttcatttga atctacataa tatatttttt ctccatctac acttataaaa atatatatta
     5701 tatttttttc aagtatatta ccttgtcctc taacagtttt taaaataact tgtccttttt
     5761 tgtttacttc atctataaat ttttctgtat cttttaattt taatatttct attttttctt
     5821 ctacgtgttc tgtgtcatct gaatcgacca aatcaactaa tcttgataag agacttgtaa
     5881 atccaaagta tctaaattta tctaaaagtt catctttatc ataatcgcca aaaaccatac
     5941 tatctaaatc cacttctatt ggcacatctc ttataattgt tgcaagtctt ttactttgaa
     6001 tagctatttc tttattttcc tctattttct tttttacact acctttaagc ttatctgtat
     6061 tctctattaa attctctata ctagaaaact cttttataag ctttatacct gttttttcac
     6121 cgattccagg tactccaggt atattatctg acttatctcc cataagacct tttaaatcaa
     6181 taaattgtgc tggagtcatc tcgtattttt caattacaga attaaagttg tattcttcaa
     6241 cttctccaac tccttttttt gttataagag tagttgtatt atctgaagca agttgaatag
     6301 catctttgtc tccagttact atgtatacct tatacccatt attttctgct tttttagaaa
     6361 cagttcctat aatgtcgtct gcctcatacc catctatttc caatctattt attttgaatt
     6421 tatctaaaag gtcttttaat ggctgtaatt gttctgccag ttcatctggc attccctttc
     6481 ttccagcctt atactcttta tattctaaat gtctaaatgt aggtgctttt ctgtcaaagg
     6541 ctacacttat atgtgttggt ttatatatat ctatcatttt aaatagcatt gttgtaaatc
     6601 catatattgc attggtcttt aacccttctt tattattcat ttctggcaga gcataaaagg
     6661 ctctgtttat tattgaattt ccatctatta taattaattt tttttccaaa ttatatcact
     6721 cctaaaaatt atctattttt gtaacatata tattatatac caatatagtg ttatttaata
     6781 ttcccataat aataatagta tattaaaatt aaatatgttg caaacaatat atttttttgc
     6841 tataataata tacatgacat gcccgaatgg ttgaattggt aaatacaatg gattcaaaat
     6901 caactgtgtt gattcataag tttttataat gttttacagt ttgatgagta ttttgtattc
     6961 aatattcctc ttgcatattc ttggaaaatc tggtaggata tacaagtagt ttggatttaa
     7021 aatataagcc gctgtggcgg aactggcata cgcatacgac tcaaaatcgt acgggaaacc
     7081 atatgggttc gagtcccatc agcggcacca ataaaaaaaa ctctttagta tatactaaag
     7141 agttttttta ttgtaatatt ctttcatctt tatttctagc cttgtataag tataaatacc
     7201 atagtcataa cttcttgaac tcctaaaata tatctaaaca aagtaggatt ttttatgtat
     7261 tcacttaata aatccatact tcttatcata ttgatttttt cccaatctct aaaaattttg
     7321 atacaaataa aaaatacaga gaactacttg atatactaga aggttattct gtaccatttg
     7381 gtattgctgt aggtgttcca gatggaaatt taccaaatgc tagagatgta gtttataaag
     7441 tttctagagt ttaggataaa gtgttgcaac aatcaattag ttacttgaag agttaaatca
     7501 gtaatataca aataaatt
//
LOCUS       DAEPLF010000165         7495 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEPLF010000165 DAEPLF010000000
VERSION     DAEPLF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.165"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..257)
                     /locus_tag="KR314_002820"
     CDS             complement(<1..257)
                     /locus_tag="KR314_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329189.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYA"
     gene            complement(364..867)
                     /locus_tag="KR314_002821"
     CDS             complement(364..867)
                     /locus_tag="KR314_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0329190.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            complement(919..2118)
                     /locus_tag="KR314_002822"
     CDS             complement(919..2118)
                     /locus_tag="KR314_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBH0329191.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKNIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEENKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            complement(2378..2566)
                     /locus_tag="KR314_002823"
     CDS             complement(2378..2566)
                     /locus_tag="KR314_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329192.1"
                     /translation="MNYHTKEELMEVLRVASSRIINCEKVQKKFSEETSHHTRFKNII
                     EAMYISKSLIMDEISKRD"
     gene            2901..3869
                     /locus_tag="KR314_002824"
     CDS             2901..3869
                     /locus_tag="KR314_002824"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBH0329193.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTKL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPNTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLRECIALSKTL"
     gene            complement(3986..5116)
                     /locus_tag="KR314_002825"
     CDS             complement(3986..5116)
                     /locus_tag="KR314_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0329194.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDINISDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(5269..6222)
                     /gene="msrA"
                     /locus_tag="KR314_002826"
     CDS             complement(5269..6222)
                     /gene="msrA"
                     /locus_tag="KR314_002826"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBH0329195.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRIEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEVLGYGEYLKLFE"
     gene            complement(6346..7251)
                     /locus_tag="KR314_002827"
     CDS             complement(6346..7251)
                     /locus_tag="KR314_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBH0329196.1"
                     /translation="MKSSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
BASE COUNT         2511 a         1276 c          718 g         2990 t
ORIGIN      
        1 gcatataaaa aatttttttc tttcaacata actcctgcaa ctccaaatat aaaaaataga
       61 gaagcaataa attttccaat aagattatta ttaggataat aactaaaaag cataaaacaa
      121 agtacaaatg taagtactcc tattgcatta aacacaattt tattagatgc tatttttcct
      181 ttattgaaaa agaaacagaa tattgacact aaatctaaaa ataaaaatac ataaaacatt
      241 ccttttaaaa cgcccatttt tataccccct aaaaattcta attataatat ccagtttatt
      301 tcatataatc gtcaatataa tcaatacttt ataacatcag tataacagta ttacaaattt
      361 atattacgaa tgtattactt cgtattcata catattataa tcacactcat acatatttaa
      421 agactcatat gttgcttttg catttatgtt ttctttttct acatataatc tcattccaac
      481 tatattttca tctttatcac atatattttt tatataatta aataaataat taaagatacc
      541 ttttcttcta tattccttat ctacatatac actctgaatc caaaggaaat ttccatttct
      601 ccaatcactc cactcatatg tatacataat ctgtgcaaca actttatcaa atactgtata
      661 tacatgatat tttccttttc tctcatctaa taacaatgct tttacacctt tagttaaaac
      721 atccatatca agttcttttc cttcagtttc tttagccaaa ttataattaa attttgctat
      781 tgtatccaaa tcatccatac gtgcttctct tatctctatt agttcttcat tttcatataa
      841 ctctatgtag tccttttgtt gctccatact tattcctcca tatctatttt atctactaag
      901 gttttttata taatatattt atagagtttt ttctatcttc ataaatgcat aatcaagtgt
      961 agcctctaaa tttccattat tcttacctcc tccttgagct aatactttac ttccaccacc
     1021 cttgccatct ataagactta ttgcatcctt taataaaact cccatattaa tatcttttaa
     1081 atttttagaa catgcaaaaa ctaaatttat tttatcatta aatttaacag ctaataatgc
     1141 aatactattt tcatattctg caattttatt tgctatctta tttacatatt tcaaatcttc
     1201 atcatcataa atttttttaa tgaccgaaat attgccaacc ttatttgctt tttcaatcat
     1261 ttctttgacc tgatatgtag atactacttc ttcaagcctt ctttttttaa ttaaagcctc
     1321 ttccacttta ctatttagat ttacaatacc atttatagca tcttcctcac tacagcttaa
     1381 atatctacat attttagtca aatatatatc cctcttcaat acttcatcca ctgccctttt
     1441 acctgctaga aactctattc ttgtacttga cttatgtttt tcccatttct ttatttttat
     1501 cattctcaat tcaccagttg attttggatg cactccacaa catgcattcg tatctaaatc
     1561 acctattttt attactctta tctcttcatc agtatttggc aaatcacgtc ttaaccaaat
     1621 cttttttagt tcttttttcg ttggaagtaa tgtctctact tgtatatttt ggcgaataat
     1681 ttcattggca aacaactcta tctctctaat tttgttctct tctaaattac cttctatatc
     1741 tactgtacta tactcatttc ctaaatgaaa acctgtcgta tttgcattaa aagttttaaa
     1801 aaaacatcca gataatacat gttgtccaaa atgttgatgc attccatctt ctcttctatc
     1861 ccaatcaata ctacacttta ccttatgtat ctttataggt tttttatcta atacatggta
     1921 tatatttttt ccatcttcat acacatttat tacatcatca ttctctattt ttcccaggtc
     1981 tccaaactga cctcctccac caggaaaaaa agctgtctta tctaataaaa catgatactt
     2041 attttctact tcttttattt caattatttc tgctgtaaaa tctcttatat actgatttgt
     2101 ataatataac ttttccatta taattctatc tccattttct taattataga ctactttact
     2161 ttttaaatac aaatttcaag ctaagcttaa tttaattgta ttaaaataga actcatccta
     2221 ttatttacta acatcttcta ctatatttta acatatagat attagtgtta acaaatttat
     2281 aattttatat tatttcaata ttttataaca agtatgctta cttattttct tatataataa
     2341 aatgtatatt aaaacatatt aaattgcaaa ataagcttca atctctttta ctaatttcat
     2401 ccattattaa tgattttgaa atatacattg cttctataat attcttaaaa cgtgtatgat
     2461 gagatgtctc ctcagaaaat ttcttctgta ctttttcaca attaataatc cttgaagatg
     2521 ctactctcag tacttccatc aactcttctt ttgtatgata attcataaca catttactcc
     2581 tttgtttttt aatttagaga aacagtatac ctcaatttaa tatcattatc aactgatatc
     2641 attattattt atatatatct tttaacactt aaatattcta actatcagtg acattacata
     2701 ttttaataat tattaatata catataatac tttaacattc attaatagag agtatattaa
     2761 cattgctttt ttaaaaattt tagtataaaa tcatattaaa taaattttct gaaataataa
     2821 aatattaaaa tatccaaaaa cagcttttga taacatctat aattttcaat gtatcgttat
     2881 tttaggggga gtgatttaaa atggcaataa agccaagaaa aatatctatt gtaggttcag
     2941 ggcatgttgg ttctcattgt ggatttagtt taataactca aggagtttgt gatgaactct
     3001 ttatgattga tatagatgaa tctaaatcaa aagctcaagc tttagattta gctgatgcag
     3061 tttcatatct accacataaa gtacatatag aaaaaggaac tttttctgac tgtaaagatt
     3121 ctgacatagt agtgataagt gttgcagact cttcagaagg gcctttacgt agacaaaaca
     3181 caactagatt agatttacta agaccaacta ttggaatgat aaaatctata gtaaaaccca
     3241 tagttgattc tggctttgat ggtatatttg tagtcatatc taatcctgtt gatgtagtta
     3301 caaattatat ttgggaaaaa tctggctttc ctaaaaataa agtcattgga actggaacag
     3361 ctttagattc aacaaaactt agaaggattt tatctgaaga aactggaatc gctcaacaat
     3421 caattcaagc ttattctatg ggtgaacatg gagattctca aatggttcct tggtctcatg
     3481 tttcaattgg tggtaaacca atcttggaca tgataaaaga caacccaaat acttatagca
     3541 atttagactt accatcaata gttgaaaaaa ataaaaaaac agggataagt ataataaatg
     3601 gtaaagattg tacagaattt ggtataggta ctgctttagt agaaattgta aaagctatac
     3661 tacacaatga gaaaaaagtt cttccagttt ctacattact agaaggtcaa tataacgaaa
     3721 gaaatgtatt tgcaggtgtt ccatgtgtta taggaaaaga tggtattgaa gaaattattg
     3781 aaatcaatat gactgaatat gagcaaaacg aatttaataa atcttgctct gttttaagag
     3841 aatgtattgc actttcaaaa acactttaga ttcaatattc tttcttacac taaaagcagg
     3901 taaatgactt attgaaaaga cttttaacta agttaatgtt taaatcattt atacctgctt
     3961 ttaattttaa tactaaaata taaacctatt ttttaccatc aaattttata actttacttt
     4021 ttttatttac ctcttgagat aacaatcctt ttttcaatcc catcaataat tctaagtctt
     4081 ctggataaag atttattcca aattcctcta aacttgatac tatactattt acactcatta
     4141 ttttattttc tatcaataat tcaactgctc tttttaaata cgttgggctt gaacctttca
     4201 ttttgtctag tggttcttct atcaaccagc cttttttatc catttcattc ataagataat
     4261 tgtatttttt gtaatttatc ttttctagat tatatgctct aaaaatcata gcagatatag
     4321 gtacaatcca ttttttcttt agttctacat aataatctaa atcttctgga tgagtcaaat
     4381 cttttaaaaa actctctttt ggcaataaaa atgcacaggc atactcatct tctgaaaatt
     4441 gcttacttgg tattcttaat tcacttgata ttatatatcc aagctcacat gccaaagtat
     4501 aatttctaat tgatgctgac tttttgtcat tacccagaga tattagatat ttagattctt
     4561 tactaattct ctgtttttga gtaaagatag ttgccccttt tctatctaca ttcatacctg
     4621 atattataat ccctttagat tctaacaaac ttaccatatt tactattgga gtactattta
     4681 aatcccaaaa ctctctagtc ttatatgcaa gagattctat atcacttata ttaatatctg
     4741 acattacttt tctatttggc aagttttctt ttggaaactt aatataattc tccataaatg
     4801 aatatatttt atgtattata attatttttt ctctataaga aatctcttct actctaggta
     4861 gtttagattg agggttaaaa tggctatcct ctacaacgat atttatattt tcatttttat
     4921 aaaaatactc ttttggaaaa tttaatatat tagataattt taatgtattt tctatatttg
     4981 gtacatactt attttcctca aaagctttta aatctttttt atttatttta gtttctttag
     5041 acaactgttc tatcgtcttt gctctaaaaa ttctagctga ttttaatctc tttccattaa
     5101 agtcttttaa attcatttgt tttctctctt tcaaaattaa attcttaatc tattggtata
     5161 aataagatta caattaataa tttatcatat tattgacaaa aaaacaaaag gtgaaaatat
     5221 ttttttatat tttcaccttt tagataagtt tataataaat attcctaatt actcaaaaag
     5281 ttttaaatat tctccatatc ctaaaacttc catattttct cttggtataa atttaagaga
     5341 agcagaattt atacaatatc ttaaacctcc aagttctttt ggaccatcat caaatacatg
     5401 acctaaatga gaatttcctt tagcacttct tacttcaatt ctgtacatac cttgtgagaa
     5461 atcacttctc tcaatgatag atttatctat aactggcttt gaaaaacttg gccacccaca
     5521 tccagaatca aatttatctt ttgaagaaaa taacacttct ccacttacaa tatcaacata
     5581 aattccctct ttgttattat cataatattc attttcaaaa ggtacttctg tcatatcatt
     5641 ttgagtgact tcaaattgta taggagttaa agtctttttt aactcttccc tatttatatt
     5701 gtttccatcc caatgacttt ttacaaaatt atatcttcca gaccctttat aatacatatt
     5761 gtagtttaat ggtctttttt tataataatc ctgatgattt tcttctgctt cgtagaattt
     5821 agttgctgga attatatcag tcactatttt actatcaaat attttactat tttccagatg
     5881 ttctcttgac tctaaagcta tatttttttg ttcttcatca tgataaaata tagcagtctt
     5941 atattgagtt cccctatcac caaattgtcc accatcatct gtaggattaa tctgtttcca
     6001 gaagatattt agaagttcat cataacttat tatatcatca tcatatgtta tttgaattgc
     6061 ttctaaatga ccagtagttc ctgaacatac ctcttgatat gtagggtttt caacatgacc
     6121 acctgtatat ccagaaacta ctttaataac tccatcatac ttatcgaaag gttttaccat
     6181 acaccaaaaa catccacctg caaaagtagc aagttttatc attttcatca ctccttaaat
     6241 aaattatact ttatttattc cctaagaagt aacttaataa tctgcatttt attattttta
     6301 cctactcaat gaaatcatgt gcaaaaatac aattgcaaat tgaatctaat ttctataaaa
     6361 gctctttttc tttatccatc caaaagcatc ttttatatca caattttctc ctaaagacat
     6421 tagatattct aatgcatcac aatcatccat agctacatct ttttccatct taggatttaa
     6481 tctatagtaa ttatctccaa gaagtttttg agaaaataac tgactcattt gagaatttcc
     6541 ttctaaggta atagatataa ttggtaaatc gggttcttta ttaattatcc agtcaatagc
     6601 tccccattca gtagtatctg cctttatact attatataca tatccagtcc ctattgaaaa
     6661 aagtcttgta ttcttaagtt taaatccact gtcaatggca tgaattatac acgctaaact
     6721 tgggtcagtt gctattattc ctccatcaac atgacgatta tatgttggaa aaaatactgg
     6781 agcagcacta cttgccattg ctacatctac aactttgcta gtctttgtaa aagaatttgg
     6841 catattgtta taaaacactg gtttccatgc attttcctca tttcctatat aaaaacttgg
     6901 tatcattact aatttattta aatcttttaa ttctagttcc tctggaaata tacttaataa
     6961 aacttctttt aagtttttgt tttcatactt tggtctgagt atttctgcat aacttttatt
     7021 aaaaatatac ttagaatttt caattgagta aagttctttt atctctttag ggcttacacc
     7081 atacgcaagt cctagtgcaa ttagactccc tgtagatgtc cctccaaaca tatttatatt
     7141 gtttaataga tttggatact cttttactat cctctcaagt atggatatac tcaatgcccc
     7201 ttttaatcca ccaccatcaa atgccaatat attaaaatta gaacttttca tatacatata
     7261 cccccacata aaacacctta tattttaatc aaaataattt cactatagta tatatatttt
     7321 atgcaagtta catattacaa tgttcctctt ttaatattta agttacattc aaacctttag
     7381 tacttaaaat aaaatatata agatattata tatctaagta ttataaattt aatacaaaaa
     7441 ttcttaaaag aatatactga aagcaggttt atttttcaat tattacaaaa aataa
//
LOCUS       DAEPLF010000166         7393 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEPLF010000166 DAEPLF010000000
VERSION     DAEPLF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.166"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..417)
                     /locus_tag="KR314_002828"
     CDS             complement(<1..417)
                     /locus_tag="KR314_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBH0329197.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKN"
     gene            638..1462
                     /gene="pdaA"
                     /locus_tag="KR314_002829"
     CDS             638..1462
                     /gene="pdaA"
                     /locus_tag="KR314_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBH0329198.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            1651..2415
                     /locus_tag="KR314_002830"
     CDS             1651..2415
                     /locus_tag="KR314_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0329199.1"
                     /translation="MTTICVMIDHIFLDTEYRQPTIHKHLAKHIIISQSGNLSVTFDK
                     GESLQCKGIIIDSNVSHTVSSKDNKMLVFLIDDTSILAQNISQHQLLNHSFFVIDNLI
                     VERIQNIYTACQVKGLSQSYIDFSKQAFRLLNLNYHISSITDSRIQWALNHILKSTHI
                     HNEIFFEICAEVKLSKSRFSHLFKEQMGVSLNSYLALSKLRKAYELLLKSGDITSAAM
                     DAGFSSPSHFSASSKKYLGITASELIGKCKIYFIRQ"
     gene            2518..3696
                     /locus_tag="KR314_002831"
     CDS             2518..3696
                     /locus_tag="KR314_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBH0329200.1"
                     /translation="MKTNKISFNKGLYYLNTNANFNFQLNRIIMWDGGRLEDIQKVSS
                     KIVDSESWKTELMYLAQEAEQDGRTENAIAYYRMSEFFMYDGDPDKISVYKKATTLFY
                     NYYSEYFSNGTVQIFHVPYENITLPVMYAKAQGEVKDVILLHGGNDSYFEEFFFPMLY
                     LASQGFDIYLFEGPGQGGVMRLQGKHFTYQWERPVKTILDYFHLENITIVGASLGGFL
                     APRAAAFEKRIERVVAWSVFPNFLDIIVGSQPIKMQNLLRLLLKLKTKWLVNFIIKQK
                     MKSGDSMVVWGLKHGMYAYEASTPYEYFQKIKYYQIDSIAKNINQDILILGATQDHFI
                     DYRTVGKEIDLLNNVRSLTFRLMTEAEQASNHCNCGNSKLVLDTISNWIMMMKIHDRV
                     "
     gene            complement(4067..4852)
                     /locus_tag="KR314_002832"
     CDS             complement(4067..4852)
                     /locus_tag="KR314_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0329201.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFDIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            5317..5682
                     /locus_tag="KR314_002833"
     CDS             5317..5682
                     /locus_tag="KR314_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329202.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            5760..6314
                     /locus_tag="KR314_002834"
     CDS             5760..6314
                     /locus_tag="KR314_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBH0329203.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            6534..7208
                     /locus_tag="KR314_002835"
     CDS             6534..7208
                     /locus_tag="KR314_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBH0329204.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
BASE COUNT         2701 a         1085 c          982 g         2625 t
ORIGIN      
        1 attttttaag tattctaagc tataatcttt atgatatggt tctaaaataa ctgctaattc
       61 aattaaatca ataaatttgt tttttaactt tggcatatga tactcaaaaa actttttttt
      121 cagacttaaa tcatgacata taatgaaact atcttctaca aaatttacaa gtctatcttc
      181 caccagttct atttcattag ctatttctaa atcattttta tctaaatttt tacatactga
      241 gaatacttct ataggtacct caaatttgtt ttttattaat gattcatagg tatatatttt
      301 ccagtcttta accttgacag ctccaacttc taaaatttct gaatttaaac aatctaatcc
      361 actaacttct atatctaaaa aaacaacatc attcaataca cttattatat tatccatttt
      421 tatatccctt caagttaaac cagcctaact tatttacaat catttctctt tatattttat
      481 catttatata cactttattt aagataatta atatcaactt tatatcttac agtaaacttc
      541 tttcatttat tatttgtata tgtgaatatt tttaactata aatattcaaa ctatattata
      601 agaaatatac tttaaatttt aaataagagg tgaaattgtg aaaaaagata gcctaaaaaa
      661 atacattatg ataggagcat ttgctctaat actttttgga attgctagta taaattttaa
      721 atcattagat aaaacaaaaa ctcaaatttc tagtccaaca ctcgacacac atgaatatga
      781 ttggtatttt aaccctaggg aggatggtaa acagccatca cctataaaag aagctgattt
      841 ttttaaaaaa tacggagctt attatgtagg aaatcccaat gaaaaagtta tatatctatc
      901 atttgatgct ggatatgaaa gtggaaacac tccaaaatta ttagatacgc ttaaaaaaca
      961 caatgctaaa gctcaatttt tcgtagttga aagttatata aaaagcaatc cagagttaat
     1021 aaagcgtatg gaaaaagaag gacatttagt ttgtaatcat tcaaaaagcc atccatcaat
     1081 ggcaggtatt acagattttg aaaaattcaa agaagaaatt acaagtgtag aaaaagcata
     1141 caaagatgtt actggaaaag aaatgcctaa gtattttaga cctccaatgg gtaaatttag
     1201 cgaacaatct ttaaaatata cacaagattt aggatataaa tcaatatttt ggagttttgc
     1261 ttatgtagat tggtatgaaa agaaacaacc tactcatgaa tttgccaaaa acaaaattta
     1321 ttcaagaact catcctggtg caatagtttt actgcatcca aattcatcaa caaatactga
     1381 gatattagat gaggttctta cacattggga aaaagaaggt tataaactta aaacacttga
     1441 ttatttaaat aataaaaaat agtatcataa attttaaaaa actctatcat gaatcatagt
     1501 aaaaatatag ttatttataa atactttaat tctcgataga gttttttatt tttcattgcc
     1561 taaaatagga tttttaaata cttgtaatca ctaagctttc ttgttaaaaa ctgtataata
     1621 tgctaagcta tgaacaagga gttgacagct atgactacca tttgtgtaat gattgaccat
     1681 atatttttag atacagagta tagacaacca actatccata aacatcttgc aaaacatatt
     1741 attatatcac agtctggtaa cttatctgtg acgtttgata agggcgaatc attacaatgt
     1801 aaaggaatta ttattgattc caatgtatcg catacggtgt caagtaaaga taataaaatg
     1861 ttggttttct tgatagatga tacaagcata ctcgcccaaa atattagtca acaccaatta
     1921 ctaaatcact cttttttcgt tatagataat cttatagttg aacgaataca aaatatttat
     1981 acagcttgtc aggtaaaagg attgtcccaa agctatattg atttttcaaa acaagctttc
     2041 cgtctattga atttaaatta tcatatttcg tctattacag acagtagaat acaatgggca
     2101 ttgaatcata ttctaaagtc cacacacatt cacaatgaaa tcttttttga gatatgtgcc
     2161 gaagtaaaat tgtccaaaag ccggttttct catcttttca aagaacaaat gggagtatca
     2221 ctaaacagct atttggcttt atcaaaactt cgcaaggctt atgagttatt gttaaaaagt
     2281 ggagatatta cctcagctgc tatggacgca gggtttagtt caccgtctca tttctcagct
     2341 tccagcaaaa aatatcttgg cattactgca agtgagctta taggaaaatg taaaatatat
     2401 tttatccgcc aatagcgtct atttgaaagt cagtacttct aatatttgtt acaataaatg
     2461 catagcatta aagattactc tttattgcta tgcattatct ttttaggagt gatgacattg
     2521 aaaacaaata aaatttcttt taataaaggt ctatattacc ttaatacaaa tgccaatttt
     2581 aattttcagt taaacaggat tattatgtgg gatggggggc gcttggaaga tatacaaaaa
     2641 gtttcctcta aaattgtcga tagcgaaagt tggaaaacag aacttatgta tctggcacaa
     2701 gaagctgaac aagatggacg cacagaaaat gcaattgctt actatcgtat gagtgaattt
     2761 tttatgtatg atggtgaccc tgataaaata tcagtctaca aaaaagcaac tactctattc
     2821 tacaactact actctgaata tttctcaaat ggaactgtac aaatttttca tgtaccatat
     2881 gaaaatatca ccctacctgt aatgtatgca aaagcgcaag gcgaggtaaa agatgttata
     2941 ttgctccacg gtggaaatga ttcttacttt gaagaattct ttttcccaat gctttatctt
     3001 gcgtcacagg gatttgatat ttatcttttt gagggacctg gtcaaggtgg agtgatgcgt
     3061 ttgcagggaa aacactttac ttatcaatgg gaacgtcccg ttaagacaat tttagattat
     3121 tttcatttag agaatataac tattgtaggt gcttccttgg gaggttttct tgctccacga
     3181 gcagcagcct tcgaaaaacg aattgaacgc gtggtagctt ggtctgtatt ccccaatttc
     3241 ttagacataa tagttggttc acaaccaata aaaatgcaaa atctacttag actactgcta
     3301 aaactcaaaa caaaatggct agtcaatttt ataataaagc aaaaaatgaa aagtggcgat
     3361 agtatggtgg tatggggttt aaaacatgga atgtacgcat atgaagcgtc tacaccctat
     3421 gaatattttc agaaaatcaa gtattatcaa atagattcta ttgcaaaaaa tataaaccaa
     3481 gacattttga ttttaggtgc aacacaagat cattttattg attatagaac tgttggtaaa
     3541 gagattgatt tactcaacaa tgtccgatcc cttacattta ggcttatgac agaagcagaa
     3601 caagctagta accactgtaa ctgtggaaat agcaaactgg ttttagatac aatttcaaat
     3661 tggattatga tgatgaaaat acatgataga gtctaaatat tgatttttaa attatacaaa
     3721 atccatctgc tagcatatcc ttattgcttt gttcaaataa tcacttgtta aaaaattggt
     3781 tcccacttac gaaatactac gtttatagaa atgaactata tcatgtaagc acacaatata
     3841 tatgataata agacccaatt actcaatgta actgggtctt tcttatgtca tagattttat
     3901 aggactcatg aaatgtaaaa gagtatttca ccgatttcca aagggaactt tacttagata
     3961 aggagaccat aaatgctaac aaacccatct actagacacc tttcaagtat ttgagcgatt
     4021 atacaataca cgttaagaat tacacgaaaa tttattattt ttatagctat ttaaaaagaa
     4081 ttgtccattc atttatctct ttgtatccta tattatgata tattttacct gctacatcat
     4141 tattataaaa aagacatgga acttttcctt ccaacaataa ttctttactc aaattgtaaa
     4201 ccatataact tgcaagtcct tttcccctat atcttttatc tgtacttaca gagaccacca
     4261 ttgccaaaaa actagtttcc ataccagttg acacagtgct aatcatagta tcatcatttc
     4321 taataaaata tgctcttaca tttccctctt ttaaatgttc tttcctttct tctatgtaat
     4381 ttttacttac tttatagtct gacctattta aaagttttcc tatttcatca atatcttttt
     4441 cctgagcatt ttctatttta tactccttca tgttggaaaa atctatttct tttatttctt
     4501 ttaatacaca aaatttatta tctagttttt cataaaaatc tttgtattta cttattagtc
     4561 tatctattac acattttttt ccacttatgc tttctacatc taaatctttt atatgattct
     4621 tcatttctcc tatatcaaaa ttatttttaa aactataaat tataagtgtt gacttatttt
     4681 ttaatattat agaggttata tttccatctt ctacctgaga ccatatatct tgatattgag
     4741 agctaaatcc ataattctca acgtatccaa ttatgaataa atttaaactt tcttcatgtg
     4801 atacatattg ccaaaattct ttttctaaag tattatctat ttttacaatc aagcagatat
     4861 cctccttcta tgttaataat gatatacctt ataatttata gtatcattta aaatcattat
     4921 attctaccaa atattctaaa tttttaaata tctatgcaat atgttcgtat ttatttttat
     4981 attctgttac tgcttcactc caagcttctt ctactgaaat acctctctta tttgcaattt
     5041 tttgtgctaa ttctattatt ttcatagctt ttcttgatat attcatatat attcctcctc
     5101 taaattatca taagttattt taaagttaaa tttctatagt catgttatat atttatttac
     5161 ttatatctta ctaattattt atgtactaaa tatgaactac aatagaagaa atttagaact
     5221 taataaaaca taaaatttat atacagttca tatatatttg tcatatcaca agacaagtga
     5281 tataatttat tatcatttta ctgggggtga tatgctatga aaataagagc tgaatataca
     5341 tgtcctcttg aaattgtaca tgacataatc aaaggaaaat ggaaaactat ccttatattt
     5401 gaattaagac aatctaataa aacattttct gaattgcaac ataatattta tggaataaat
     5461 caaaaaatgt tgattcaaca attaaaagag ttacgtgatt ttgggattat tgataaaata
     5521 agttctgctg gatatccatt acatgttgaa tattttctta ctaatcgtgg taaaaaaatg
     5581 ttaaaagcag tggaaataat gcaagaaata ggaatcgaat atatgcttga acatggtcaa
     5641 caagaaattc tagataataa aggaatttgc tacaataagt aatcctatac ataccaaaaa
     5701 gtaagtaatt tacatttttt acaatagtat tataataaaa gtaaatagaa ggtataatca
     5761 tgtctatagc tttaatattg attgatattc aaaatgatta ttttaaaaat ggaaaatgtg
     5821 aattatttca atcagaagaa actgctgaaa atgctaaaaa aatactactg ttttttagaa
     5881 aaaacaattt accagttatt catatacagc atatatcaac agataaaaca gcttcttttt
     5941 ttcttcctaa tactcatggt tcagaaataa ataaaattgt ttttccacta gataatgaag
     6001 aaattataat taaacacaca ccagatagtt tctttgaaac agatttgcaa agtatccttg
     6061 agaaaaaaaa tattactaat ctagtaatct gtggaatgat gagccatatg tgtattgata
     6121 caagtgtaag aacagcaaag aaacttcgat acgacataac tctaataagc gatgcatgta
     6181 caacgaagaa tttatcttgg aataatatag aaattaatgc aaatactgta catcaagtat
     6241 tcatggcatc actacagaac agttttgcag atgtaaaaaa cacagataaa tttttatcta
     6301 actatcacaa ataaaaacat acactatgtt aaaagagcaa gtttgattta tttaagcttg
     6361 ctctttaatc atttaaaatt agatgctttc tatcattttt atatacataa tatacgttta
     6421 tacattttat attcatatat aactattata taaattttaa actttactat attcacataa
     6481 aaaatgtagt attatagtat atataatatt atatagaagg gggtgctaga taaatgaacc
     6541 atcatatatt gattcaattt gaatatatag caagaatcat tactgctggt ttctgtgggg
     6601 ctttaattgg atatgagaga aagaatagac taaaagaagc tggaatacgc actcatttta
     6661 tagtggcatt agcttcagca cttataatca ttgtttccaa gtatggattt aatgatatag
     6721 tgggtactcc aggtattggt ctagacccag cccgcgttgc agcacaagtt gtcagtggta
     6781 taggtttttt aggtgctgga ttaatattta ttagaaatca atcagtaagt ggtcttacta
     6841 cagctgctgg tatatgggca acagccggta caggtcttgc cattggctct ggattatact
     6901 tagttggtat tgtttcaagt atcattattg ttattgtaca gataacactt catagagata
     6961 ggaagtggat gaaactccct acagcagagc aagtttctct taaaatttct aacagcactg
     7021 attccataga atatattgaa ggtaaattta aaaaatctaa tattgaaatt ataaacatga
     7081 atattaaacg ccttgatggg gattggttag aagtagttat atatactaag ttacctaaag
     7141 gatataaaaa aacaaattta ttaaaattat ttagcgataa tcctcttata gaaactttag
     7201 aaatataata taaaaattga agcttaaaaa taataatata tttaatcaag gtcaatatat
     7261 aaataattac gggatgagtc tatataaaat tcatcccttg ttttttgcca ttcatatata
     7321 ttttttcaag atatttttgc tcatctatta tctagccaag taactcttgt gaactaaact
     7381 ttttaataca aaa
//
LOCUS       DAEPLF010000167         7357 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEPLF010000167 DAEPLF010000000
VERSION     DAEPLF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.167"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            177..674
                     /locus_tag="KR314_002836"
     CDS             177..674
                     /locus_tag="KR314_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0329205.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            724..1248
                     /locus_tag="KR314_002837"
     CDS             724..1248
                     /locus_tag="KR314_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH0329206.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKDLQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKVPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILNYIRKL"
     gene            1560..2924
                     /locus_tag="KR314_002838"
     CDS             1560..2924
                     /locus_tag="KR314_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBH0329207.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSNFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVENTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            3063..4229
                     /locus_tag="KR314_002839"
     CDS             3063..4229
                     /locus_tag="KR314_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH0329208.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            4346..5128
                     /locus_tag="KR314_002840"
     CDS             4346..5128
                     /locus_tag="KR314_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBH0329209.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYAESLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(5247..5900)
                     /locus_tag="KR314_002841"
     CDS             complement(5247..5900)
                     /locus_tag="KR314_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0329210.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(5924..7288)
                     /locus_tag="KR314_002842"
     CDS             complement(5924..7288)
                     /locus_tag="KR314_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBH0329211.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2818 a          875 c         1146 g         2518 t
ORIGIN      
        1 tgattgtaaa tatgatttta tgaaacggta tagccataat gctatgccgt tttgccattt
       61 ataataaaaa taaatttatt gacacaatag taactaatta ctataatata aagtaactag
      121 ttactattta aaattatcaa aaaattaaaa tctaagataa agagaagtgt ataataatgg
      181 atgcagaaag tttaatgaaa aaatatatgg tagatgatga tagtagaaaa caagctatat
      241 ttgcaagtat atttgtaatg caaaataaac ttcaaactac atgtgataaa ttagacccag
      301 aattaacaat gaaacaatgg ttacttttag ctattgcgga ctctgtagat gaaactctta
      361 cattaaccaa acttggagaa ttaatggggt gctcaagaca aaatgtaaaa aaactagcta
      421 ttgctttaga aaagaaaggt tttatacaaa ttgagcaaaa tgaaaaagat tcaagagcta
      481 tctgtcttgt gacaaaagat agagtgcaag agtattctaa aaaagtaaga ggtatgcagg
      541 aaaaagtatt acagctttta tttgaagatt ttacagagaa agaagtggaa cagttatttt
      601 ttggaattat gaaattaagt ataggaattg aaaatgtgaa aaattatgta gaaaataatg
      661 acgttggaga ataagcaagt atcaaatcaa ctttgtatga catcagagag gggggtaatt
      721 tatatgaaaa gtgtagtagt gatttataag agtaagtatg gttcaacaaa aaaatatgct
      781 aaatggcttt cagaattatt gtcttgtgat atttttgaaa ctaagaatat taatatagaa
      841 aaggttatga agtatgatac gattatttta ggaggaggtc tttatgcttg taatatagcg
      901 ggaatttctt tcttaaaaaa aaattataaa gatttgcaga aaaaaaattt agtagtattt
      961 ggcgtaggag ctgctccata tgatgatgaa gtaataaaga aattaagaga agtaaacttt
     1021 aaggatgaat taaacaaagt tccttgcttt tattgtcgtg gggcatggga tgagacaaaa
     1081 atgtctttta tagattatac tctttgtagt ttttttaaaa aatctatagc taaaaaagaa
     1141 cgtcaaggtc ttaaaccagc agaagcacct ttattgtctg gagaagcctt tgattggtgt
     1201 aataaagaaa atttaactcc aatacttaat tatataagaa aactttaaga ggtatatatt
     1261 tataattatt tattatctaa agttagattt catagaaact tactagacat tatttataaa
     1321 ataatattat taaagatgga taatttaatt gtttcaataa ggttataatt ttaaaaaatg
     1381 tttattttaa aataaattat tttataagct tacttatact taataagggg ctttttttat
     1441 tggaagattt atgttatatt gagatgaatt tattatgata taaaaaataa tgttaggata
     1501 aatttaaatc tttttctaaa aatgtattaa tataattctt aaagaaagga tttttaacaa
     1561 tggatataaa agagttagtt aaaatgcaaa gaaaatatta taatacaggt aaaacaagag
     1621 atatatcgtt tcgtatagaa cagttgaaga aacttaagtt agttgtatca caaaatgaag
     1681 aaaaaatact tttagcacta aagaaagatt taaataaatc gaattttgaa ggttttatga
     1741 cagaggtagg catgttttat agtgaattaa actttgctat aaagaatata agaaaatgga
     1801 gcaaaataaa aagagttaaa tcaagtatgg ttaattttcc atctatatcc aaagttgttc
     1861 cacaacctta tggtgttact ctcataatgt caccttggaa ttatcccttt caattagcac
     1921 ttataccatt agtttggtca ttagcagcag ggaattgtgt tatattaaaa ccttctgaat
     1981 attctgtgtc aacatctagt gtagttaaag acatagttga aaatactttt tcaaaagaat
     2041 atgttgcagt tgttcaagga agtcaagaag aaagtgaaaa gttactattg gaaagatttg
     2101 attatatatt ttttacagga agtactaatg ttggtaaaat aatcatgaaa tcagcatcag
     2161 agcatttaac tcctattact ttagaacttg gaggaaaaag tccttgtatc attttaaaag
     2221 atgctaatat agaccttact gccaaaagac ttacttgggg aaaactaata aatgcaggac
     2281 aaacctgcgt ggctccagat tatgttttag tgcatgaaga tagaaaaaat gagttaatag
     2341 agaaaataaa atattatact aataagtatt ttggagataa tccatgtaat aatgagcaat
     2401 ttcctaaaat cataaatcag agacatttta atcggatatt atctcttatt gataaagata
     2461 agatagtcta tggtggaaat tataataagg agacattaaa gatagagcca actatagtag
     2521 acaatgttaa ttgggatgat aatataatga aggaagaaat atttgggcct atatttccca
     2581 ttttaacata taaagattta gatgaagtaa tacaaaaaat tatacaaatg ccaaatccat
     2641 tggcacttta tatttttaca aaaaataagt acctagaaaa taaactttta gaaatgattc
     2701 cagcaggcgg atgttgtatc aatgatacag ttactcatat agctacgaat tacttacctt
     2761 ttggtggtat tggtgaaagt ggtatgggct catatcatgg aaaagctgga ttcgatacat
     2821 ttactcacta taagagtgtg ttaaaaaaat taaatttgga tgtccctatt agatatgctc
     2881 catacgataa taaattaatt aaggttctta aaaagataat gtaggttata ttgtagaata
     2941 ataaatataa ataaaattga gtatttgtat tctgaatata ttttattttt aatttggtat
     3001 atttgttata aaagtggtga ttttgtgata aaatttaatt atatagaaat aaggaggaaa
     3061 aaatgaaaat taattggaat acaaaataca caacaatagc aatttataca tttataatag
     3121 ctgctagtag tattatattt tatctagtat catcacaaat tgatgtattt tctaataatt
     3181 tagatgctat atttacaaca ttacaacctt ttataatagg atttgctatt gcatatcttc
     3241 ttaattttat tttaaagttt tatgaagaca gaatttttat aaaaagtgag aaattaaaaa
     3301 aactaaaaca aagttcaaaa agaggcttgg gtcttttatt aacatatgca acagctacat
     3361 tgatacttta cttatttatg catttcgtat tgccacaagt aattgagagt attgttggac
     3421 tagcaaatga tatacctatg tatgtaaaca atgcaactaa gcttattgat aaattaatga
     3481 ctgatttaaa tcttgatgag caatacttta atttggcagt tgataagtgg aatgagtttg
     3541 ttacatatat tataaagttt gtaacagatt taatcccaat attaggaagt atgttaaaga
     3601 atgtagcctc tagtatatgg aatattgtac taggattaat tgtttcagta tacttattaa
     3661 ttgataaaga aaaattctat ggattaagca aaaaaataac ttatgcagta tttacagaaa
     3721 aacaagctgc cagaatttta gaattaactc atagaagtaa ctatacattt ggaagatttt
     3781 taggtggcaa aatattagat tcattcatta taggtattct aacatttgta atacttacat
     3841 tagttaaaat gccatataca ttattaatat cagttataat tggtattaca aatattatac
     3901 ctttctttgg acctttattt ggagctatac catctactct aatagtgatg tttgtatctc
     3961 ctataaaagc tttctggtta ttgttgatta tattaataat acaacagata gatggaaata
     4021 ttataggacc taaaatactt ggagattcaa ttggtatatc tgcattttgg attttgttct
     4081 ccttattggt tgcaggaaaa ttattaggtt ttattggaat ggttatagga gttccaatgt
     4141 ttgcagtaat ttattctata attaaggata ttgttgaaag taaattggac aaaaagggat
     4201 taccgacaga tacttccgat tacatgtagc taaaaaatat tatgtataat taaaaatata
     4261 taaattaagc aatatttaaa gcactgagaa agttaaactc ttggtgcttt ttagatataa
     4321 aattattata gaagaggtgt tttttgtggc atttatagaa tgtaaattta aatctaaata
     4381 tactggagga gaaactgata taattgtaat tttgcctgta gcaaatggaa aagaactttt
     4441 tagtggtaaa gagatatatg attttgaagg taaatttaaa acattatact tatttcatgg
     4501 tttagaagat gaccaaagct catggattag atatactaat gtagagcatt atgctaaaaa
     4561 taaaaatata gccctagtaa taccaagagt agaaactagt ttttatacag atatggcgta
     4621 tggacaccaa tattttactt ttgtaagcga ggaattacct aaatttgtta gagctatatt
     4681 tcctttatca gataaaaggg aagataactt tgtagctggt atgtctatgg ggggatatgg
     4741 agctttaaaa ttagcacttt caaaaccaaa agagttttcg gcagttgcaa gtttttctgg
     4801 ttcgcctgat atgattcatg agttagaaaa tcaacaaata gaaaccaatg cagacatttt
     4861 atttcacgat tttggtagac cagaagacgc tagaaatagt gagaatgatt tactttacat
     4921 acttaagaac cttaaagaaa gaaatgaaga tattcctaaa atttatcaat tttgtggtga
     4981 tagagatttt ttatataaaa ataatcagat gtttaaaaaa tatgcagagt ctctgggtgt
     5041 agatgttgaa tatgaagagt gtgtaggggg gcatgaatgg cgtctttgga atttatggat
     5101 aaagaaattt atagatataa ttgggtagta cacttatggt attaacaggt atactataaa
     5161 atatctttaa atatgataaa gactgaaatc ctaaaatcaa attatgattt taaaatttca
     5221 gtctataaga atttaataaa attttattaa gttcttacac gagttattat tggagtaaaa
     5281 ttgtcaacta tatctatata gtatttactc aattcctcta aaatctcttc gcataaatcc
     5341 gtactatgac caagattaat aagaaactgt tttgtaaaat gctttattac agcttgagaa
     5401 gatatatcgt acttgtgtga agtataaact ctaattattt caggtttcat agcaagtata
     5461 ccaatacaag aaagtattaa ttcatcatgt tctatagatt ctttatcatt aacatatctt
     5521 ttcatgacca tttcggttac acttctttga tagcttaaaa tagcttcttc ataagctata
     5581 tccaaataaa aatttctata tgcttcgttt tttagaagga aacaaaggtc agcattcata
     5641 gcaagaaaga acatcatcat agtaccttct tcatggtagt tatagattaa agactgcatt
     5701 tcatttctta tatcttcata tattatagct cctatttcgc ttttacttga gaagtaatag
     5761 cttattagac ctaaatttat attagctctt tctgcaattt gcctagttgt tgtcttatga
     5821 taaccttgct caaaaaataa ttctctagca gcacttataa tactagcttt tgtttctttg
     5881 cccttttctg attttgacat atttactgtg tatacctctc ttttcaatct ttttttgaaa
     5941 atttgctaac tataaatatt gtaatagaac cacctattat aggaaccaaa attctgtcaa
     6001 atacagtaag aataccaatc atatttaata atgccaatat aacacttact ataacagcat
     6061 agatagctcc tttttttgta gcgcctttcc agcaaagacc aattacaaat ggtgcaaata
     6121 ttacacctgt acataatgga gccggccaaa acaataattc taataaacta tcaaatttaa
     6181 gtgctatgat tgcacctatt atagctgtca aagctgatgc aatttgagaa tactttagag
     6241 tattttttaa atcattaaat tctatattag gatgtaataa tttgttataa atatctcttg
     6301 atataatagc actcattgag gtcattaatg aatcggcagt agacattatg gcagccaata
     6361 tagcagctaa gaataataca gatattaaag gtggcaaagt agtcgatatc atttgaggta
     6421 aaacctgaac agatgatatg tttggaaata aactaccacc aatagctcca attattccta
     6481 tagggaaggc aagaaataat agtataaatc ctgcaattac actactttta actgcatttt
     6541 tagcattatc agcacattga tatcttaaaa aactttcata agaaaatatt gagcctaaaa
     6601 ctaatggtaa aactgtatac ataaaatcac taatcgattt atttcctgga aagaatggtt
     6661 cttttaccat tgattgtaac ataagtaaag ggttgtcgcc aactaatttt aagcttatat
     6721 aagctactat tggcaaaaac actatacaaa tagcaagttg aataaatgct gatactgcaa
     6781 ctccccacat tcctgatgta taagtatata taattataac tataactcct ataattacag
     6841 ctatatcata aggaatacca aatgcagtag atacagaacc aaaagaaatt aattgactac
     6901 ccataatagc tagtatagat attggtgtta caagactttg ataagctctt aaaaatacac
     6961 tatgaccata tctagtttca atataatcag caggtgttat atcacaagat aagcttctta
     7021 attttcctgc gaaaaaggca tttataaagc aagctaaagc tgcacctata gtagaataca
     7081 ttccaaccca aagtcctctt tcagctcccc attgaactcc accaactaag caccctcctc
     7141 caaaagatgt agcagcaatg gttgcagcta gtataggcaa aggaagattt ctacctgcga
     7201 ttaggaaatc ggatgctgtt ttaacttttt ttgaaaaata aaaaccaatc catatgtata
     7261 ttgcacacgt aacaattaaa agtaacatag tcttttctcc tttataaaat attttaataa
     7321 aatatataaa atatatttaa aatatatttt atataaa
//
LOCUS       DAEPLF010000168         7316 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEPLF010000168 DAEPLF010000000
VERSION     DAEPLF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.168"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            384..2429
                     /locus_tag="KR314_002843"
     CDS             384..2429
                     /locus_tag="KR314_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0329212.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNILIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILNSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            2690..3889
                     /locus_tag="KR314_002844"
     CDS             2690..3889
                     /locus_tag="KR314_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBH0329213.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDICRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARNLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            3941..4636
                     /locus_tag="KR314_002845"
     CDS             3941..4636
                     /locus_tag="KR314_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBH0329214.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            4649..5326
                     /locus_tag="KR314_002846"
     CDS             4649..5326
                     /locus_tag="KR314_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329215.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            5437..7038
                     /locus_tag="KR314_002847"
     CDS             5437..7038
                     /locus_tag="KR314_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0329216.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDDLRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDNKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGIIKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
BASE COUNT         2939 a          715 c         1343 g         2319 t
ORIGIN      
        1 agtaatatta tgtgaaaaga tttaagctaa attttatgat gttttttaca ctaaacaaat
       61 agggttttag aacttgtaac taagttctga gaccctatta ttatatattc ctaaaatttg
      121 ttataattta ttccttgtca ttcttaaaaa tgtattaata tattaacgaa tttatattaa
      181 atttttgttt atattccaat aatgtcaaac taaccgtttt agaaattaaa ttatatgtga
      241 aaaaagatag cttacattat tttattatgg aaaataatag taaatggtac aaaatttgat
      301 ttttaagatt taaaactttt ttaaaaagtg ctacaataga ccaaattatt actttctata
      361 ctcgttaaac aggaggtggg actatgaaaa aacataatat tttatttgta tcaactgatg
      421 acaaaataaa tatagatata tcaaaacaat tggaaaatat atttggagaa ttttgtaata
      481 tagataactt ggtatatgta aataggataa atattgaact gtcaagctat gagcttgtag
      541 tatgttcaga caatgatata aaggaatata tacataataa tattgataaa aatatactta
      601 ttgtaatagt acatcgtact ataaatattg aaaatataaa tcagataata agtatagaaa
      661 atgattctga tgttatggtt atagatgctt ataaagagtc agcagatgaa actgccaaga
      721 taattagaaa actaggatta attcatatta atctaatacc atattatcca ggttgtgata
      781 agtctaaatg tgagattgga attattacgg gaagcagaaa cagcatacca caaaacataa
      841 agcaaataat tgacataggt gataaggtaa ttgatataaa tacagttata gaaatcttta
      901 caaaactaaa tatatctata gataaattac atattattaa ggaaaaatac aatgaagata
      961 cagtaagtgg atataggtat tatacaacta tgaataaaac catgaagagt tttttggaga
     1021 ttatagatga aggtattgcc tctgttgata aattaggaaa atttatatat tgcaacaaag
     1081 tattttctaa tctagttgga atagaccaaa atgaaattat atctaataac tttatggact
     1141 tatttagtga taaggttgtc aaaaaaatat tttttcaaga agatgaagtt aatgatgaag
     1201 tagtaaattt aaataacaaa aaactgataa ttaataaagt aaatgtttat gaaaataatg
     1261 aacgaataaa gagtattata agcattaaaa atataagtgc aattcaagtg cttgaggaca
     1321 agattcaaaa taaacttcag tcaaaaggtt ttgtatcaaa gtatacattt gagagtgttg
     1381 taggtgagag taaaataata aaagaaaaaa ttaatattgc aagaaaaata gcaactacag
     1441 acttttctgt gttgatactt ggagaaaatg gaacaggaaa agaaatattt gcccaggcaa
     1501 tacataatga atctctaaga aaaaacaagc catttgttgc agttaatcta tcttctttgt
     1561 cagatacact tattgaaagt gaactttttg gctatgatga aggaagtttt acaggagcaa
     1621 taaaaggtgg aaaaatgggt atatttgaaa gagcgcatac aggaacaatt tttttagatg
     1681 agataggtga tatatcttta gatgtacagc agagattgct aagagtatta caggaaaaag
     1741 aagttatgag aatgggcgga agtaaaatta taccaataga tgttagaatt atagctgcaa
     1801 caaataaaga tttaaagaaa aaaattaaag aaggttcttt tagagaagat ttgtattata
     1861 ggattaatgt attacatata gagataccta gacttagaga acgtaaggaa gatatacctc
     1921 ttataagtaa gtatttttta gatgaaataa atagcaacaa atgttttaca gaggaatcaa
     1981 tgcaagcttt aacattgtat gaatggccag gaaatgtaag agagcttaaa aatttaattt
     2041 attatattga tactatagtt gaggaagata gagtagatta cgagcatttg ccagaacaat
     2101 ttagatttga aaaaaacaat actttagtta atgaaaattt cgattcaatt atcttagatt
     2161 tcaaacaatc taatttcttt gaggaaagta tatgtatatt gaacagtata gaaacttgga
     2221 ataataaaaa tatattatta ggaagaaata aactgcaaga gattttaaaa gaaaaagata
     2281 tagttttaag tgtagaccaa attagaaaaa gaatagataa gttaaagagc catggtttac
     2341 ttttatctgg agtaaaaaaa caaggtagtt ttataactga tgaaggaaag aactttatat
     2401 catatgtaaa aattaaggga gtgatatagg ggactaaaaa ggcaaaacac cctaaaaaaa
     2461 tccctataat ggatagaaat aaataaaatt atctttaaga ttaagataat tcaaaaatat
     2521 tgaataaata atctaagaag attattttta ataaaacagt agtaaatatc aaccttacta
     2581 ctgtttttat tttttgcaag atttattata caattaaaat ttatattttg gcatatcaat
     2641 tgctacatag gaaaaatata aaaaattgta gaacttggag gatgttgtta tgaagtcagc
     2701 ttacaaagga tatgaagcct atagttattt agaaaaagga aaagatattt catctattga
     2761 gatgtgtaaa ggagataaaa gagttaaaga gtaccttatt gaattagatg atattcaaga
     2821 agctaaggtt aaaagtatag tggatgaaaa aatatttata tcattacatg aacatccagt
     2881 attatttcca gaacacctag aaagagatat atttgaatat aatagggaag gtaaacaaag
     2941 atgcgcttat gaagcattat caagaggata ttatgatgct atatttgata atttaatgga
     3001 tggtgtatgt acgataacat caaattcagg gtggaaatgg gatgatatat taatagattt
     3061 aggcatgaga ctatgtgatt tagctcatca agattttgtc ataaagtgtg aaaaagtaga
     3121 agatatatgc agagctaaaa gtgaaggtaa agtagcattg ataccaacat tagaaggtgc
     3181 agcaccaata gaaaatgaac ttgatagaat agatatactg tatggatttg gtataaggtt
     3241 aatgggtata acatacagcg aatccaatgc attaggttca ggactaaaag aagagaaaga
     3301 tggaggtctt acaaatttcg gaaaaaaagc tgttgaaaga atgaataaaa ttggaatggc
     3361 aatagattgc tctcacgttg gagttcaaac tacacttgat gtaatagaat atagcaagga
     3421 cccaataata ttaagccatg ttggggcaag aaatttatgg aacacaaaga gactaactcc
     3481 agatgaagta ttaatagcat gtgctaaaaa aggaggggtt ataggtgtag aagcagcacc
     3541 tcatactaca ataacagaga agaataaaga acattcaata gagtcattta tggaacattt
     3601 tgagtatatt aaaaatttag taggaataga ccatgtagct tttggaccag atacagtata
     3661 tggcgaccat gtaaaattac actcagtact agctgctaaa tttgatataa aaaaaataac
     3721 aggtacaaaa gaacatccca aagtagaata tgtaaaaggt cttgagaacc caacagaaac
     3781 ttcaaaaaac attgtcagat atttagtaaa gcatggatat agtgatgagg aaatagagaa
     3841 ggtactaggt ggaaatatac ttagagtact aaaacaggtt tggaaataga tatattagaa
     3901 atattaataa aatttgagga tttataggga ggttttataa atggattatt taaaattagc
     3961 taatcatcca cttttgtgga tagcgagtac agtagcagta tcaatagtag tggtacaatc
     4021 ccttatattt gctataaaat cttgcaaagt tggtaaaaca atgggaataa cagataaaca
     4081 aataaagtca gctgttaaga gtagtgctat ttcagctatt gggccttcaa tgactatatt
     4141 tgctggaatg gtatcgctta tagtaactat gggtgggcct atagcatgga tgagattatc
     4201 atttataggt tctgtaattt ttgaatcaat gtcagctgga tttggaacag atgcattagg
     4261 tataacttta ggttctcctg aaatgacaaa acttgcattt acaaatgcag tttggactat
     4321 gatattaggt tctttaggat ggataatatt cacattacta tttggacata aactagataa
     4381 gataaccaat gttatatcta gtggcaagaa atcatttata ccaataatat ctctaggagc
     4441 aatgttgggt tcatttgcat atctaaatgc tgatagggtg ttgcgttttg ataatggaac
     4501 aattgcatgt ataagtggaa tggctataat ggtaatattg tgtatgcttg aaaagaagaa
     4561 aaatataaaa tggttaagag agtgggggct tacaatatca atgtttagcg gaatgataat
     4621 agcttcagta atataagggg ggagttttat gagtaaagta aaatctatat acaatgaaga
     4681 atatttacct tttatgataa ggtatggaag actaacacta tctttaggta ttatagcagc
     4741 attagttcct ggaataattt tgagctttgg atttggtatt atgcctccaa tatcagcatt
     4801 attagcaagt acaatggcaa ttgtcagtat gagtgctcca aattacataa tagaaccagt
     4861 ttcttattca ccaatcttag gtatcccagg aacttatatg agttttcttt ctggtaatat
     4921 atctaatatg aggctaccat gttcaatagc agctcaaaag gcagcagaag ttgaatcagg
     4981 tacagaagaa ggctcgataa tatctacaat aggaatagct gtatctattt tagtgaatat
     5041 aagtatacta actatagggg taattttagg aggaagcgta ttatctaaaa ttccagctga
     5101 agtagtagaa aaattaaact taattttacc agctctattt gggtcagttt ttggacaagt
     5161 atttttacaa gataaaaaat taggtctagt agcaattgta atatctgtat tgactattat
     5221 attatcaaag caaggtataa taccacagtc tctagtagtt cttatatgtg tatttggtac
     5281 tatattaatt gcaagagcta tgtataaaga taagcttagt gactaattac aatgaaataa
     5341 tagtgaattt tcaaatatga aatataataa tttgattttc aaatataaaa attacattaa
     5401 gattatttaa aaattggttt ttaaggaggg aatattttga tatatgattt aatcttaaaa
     5461 aatggattta taatagatgg tacaggtaat cctggttttt atggagatat agcaattaaa
     5521 gacaacttaa tagcaaaaat agattctaag ataaatagca atacaaataa agagattgat
     5581 tgttgtggaa aagttataac acctggattt attgaccctc atgttcatga agaaatagta
     5641 gcaatactag atggtaaatt tgaaaagttt ctaaaacaag gagttacaac aactataaat
     5701 ggtaattgtg gacattctat aacaccatat tcttctgaaa atgtatatga atatatgtat
     5761 aaaaatggtc tattacttga agaagaaaaa aagtatctta tagataaaaa taaatgttgg
     5821 aataatttta cagaatattg tgaccttata tctaaaagtg gtatttctgt caatatggga
     5881 ttcttgttgg ggcatgggac tataagatgg agtgttatgg gaggttctaa ggatagacct
     5941 cctacagaaa aggaaaaaaa tgaaattaca gatataataa atgatggaat gaaaagtggt
     6001 gcttttggaa tatctactgg attggcatat ataccaagta agtatgctga tattgatgaa
     6061 cttgttgata ttgcaaggca gattaaagag catgatggta tatatacatc tcatattaga
     6121 gattacatag gaagatataa tgcagtaaaa gaagccatag aggttgggca aaaaagtggt
     6181 gctagggttc aagtatctca cttaagtcca gttgaaatag aagcatttga tgagatatta
     6241 aaggctaggt ataatggtgt tgaaataatg gttgacacag taccaagaag ttctgggcat
     6301 tgtatgaaga aaaaaagagt aattcaattt attatggcaa tatcatctag tctatttgaa
     6361 ttaggtatag atggggtaat ggatgatctt agaaatgaag agggaagaac acttatatta
     6421 aaagaagcat ttatacttgg agataggggg agtataatac ttctaaacac taaagatatt
     6481 aatatggaaa agaaatcaat acgtgaaata gctacacaaa aaggaataga tgaggacaag
     6541 ttattactcg atttactttt agatggagac gaagaactta tcttttgttt aggaggaatg
     6601 tatagagctg atttcccaga taaactacat gataataaaa taattgataa tccatttgtt
     6661 atggttggtt cagattgttt gtttagtgtg ggtggggata tgtcatggtt tgaactacaa
     6721 agaagaggag cttttccaat tttcttcaat atgtacagaa agtctggtgt tagattggaa
     6781 gagattgtaa gaagagttac ttcattacca gctaggcaat ttaaaataaa aaatagaggt
     6841 attattaaag aagggttgat agctgatata gcagttattg acatggataa ctatagttat
     6901 tctaggtcag aagatattga tttttcaaag ccacaattac tagccaatgg tgttgaatat
     6961 gtaattgtaa atggaaaaat tgcgttagaa aatggagaga ttactgagaa taaatgtggt
     7021 gaagtattaa aaagataagg ttttaaaaat aataggattt agaaagtaaa taaatatcta
     7081 agtaagtaaa gacgaagttt gggatttatt taaataaaaa tcatgcttcg tttttattta
     7141 tagtattaat atttatttta ataatcagat aatatttata caaaaaatat ttatacttta
     7201 tagtgaaaaa attaatattt ttaatcatta aattatgtaa aaaattggat aaatagataa
     7261 ctaaatcatt tgagaaaaga tttttagaaa taaaaaatga taactaaata acccct
//
LOCUS       DAEPLF010000169         7269 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEPLF010000169 DAEPLF010000000
VERSION     DAEPLF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.169"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            138..1409
                     /gene="sleC"
                     /locus_tag="KR314_002848"
     CDS             138..1409
                     /gene="sleC"
                     /locus_tag="KR314_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBH0329217.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIVAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGADTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(1879..2535)
                     /locus_tag="KR314_002849"
     CDS             complement(1879..2535)
                     /locus_tag="KR314_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329218.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(3728..4138)
                     /locus_tag="KR314_002850"
     CDS             complement(3728..4138)
                     /locus_tag="KR314_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329219.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(4354..5736)
                     /locus_tag="KR314_002851"
     CDS             complement(4354..5736)
                     /locus_tag="KR314_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH0329220.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKSGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIEQSNYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(5747..6106)
                     /locus_tag="KR314_002852"
     CDS             complement(5747..6106)
                     /locus_tag="KR314_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0329221.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(6311..7090)
                     /locus_tag="KR314_002853"
     CDS             complement(6311..7090)
                     /locus_tag="KR314_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBH0329222.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         2551 a         1220 c          755 g         2743 t
ORIGIN      
        1 ttaaattatt taaatacaaa tatactcaaa tactcaaaaa aatttaaatt tatatctata
       61 ttatttaaca aatttcaagc tatattcata tcttgaaata taagggaact aaattcattt
      121 aaagaaaggg tgatttgatg caagatggtt tcttaacagt aagcataatt gatgctacta
      181 acaatagacc tattcaaaat gcagtagtaa acatatattc aatgtctaat ggtagccaaa
      241 gctcatcaac tttataccaa aacctaagaa gtaatgaatc tggtcaagtg actggtttag
      301 tattaccagc accagatgta gattactctc tacagccatc tgatgttaga ccttatagcc
      361 agtatatagt ggaagccata gcagatggat atgagacagt tgtcatagaa ggaacgcagt
      421 tgttagctac aattgtagca agacaaggag ttcccatgtc tcctagaaca agaagtaagc
      481 gttctttctc aagacaaagt gagttgattt ttgacatagg agaacatact ctatatggta
      541 cttatcctcc taagattcca gaaagtaact taaaaccatt accacctcct actggtttcg
      601 ttgtattaga taaccctgta gtgcctgaat ttatagtagt tcatgatggt ttgccagaag
      661 attcatcagc tccaaactat tggattccat ttaaagagta cattaaaaac atagcgtcat
      721 ctgaaatata ttcaacttgg cctgaacaga caatctatgc aaatgtaata gctataatat
      781 catttacttt aaatagagtc tttactgaat ggtatagaaa taaaggatat aactttacaa
      841 taacttcaac aacagcatac gaccataagt ttataaataa tagaaactta tttgaaccaa
      901 ttaacgtagt tgtagatgct atttttaata cttttataaa aagacctcct acatcaagac
      961 agccactatt agcacaatat tgtgatggac aaaaatctca atgtcctgac caaatgactc
     1021 aatggggcag taaagactta ggtgaccaag gatatgacta tgagagtatc ctaagatatt
     1081 tctatggaga tgagatagta tttgaaagag cacctatagt aagtggtgta ccagtgtcat
     1141 tccctggtac tacactacaa gttggttctt ctgggcagta tgttagaacc atacaaaatc
     1201 agttaaatgc tatatctaat tcttatccag ctgtaccaaa agttatagaa gatggtatct
     1261 atggagcaga tacagaaaac gctgttaaga tattccaagg aatctttggt ttacctcaaa
     1321 gcggagtagt agattttaaa acatggtatg aaatatccag agtttatgta gctacaacta
     1381 gaatagcttc tttaaatcct ttaatttaaa gcttgattta gaacataaac tcaaattatt
     1441 gaataaaatt attaatttaa aataaaaatg gctgttatga actaatatat accataagta
     1501 tttttactta taagtattgt agtcttaaca gccatttttt agttataact tgttttttta
     1561 gaatcttaaa actttaaatg ggggatttta agcttcttaa aaacagtgct tagcaactta
     1621 gatttcttta aaatattata tttattttta agtttaataa aatgctattc taacgaaaag
     1681 cttacttttt atttaaaatc tcacttatta tttaaaatgc tacttctcac ttagaatatt
     1741 actccttatt atttaaaaca ctatctatca cttagaatat tccttcttat ctaaattatt
     1801 atttattata aatataaata atattaattt aaaaagttat tcataattta aataatacta
     1861 ttatcttcat cagtgaacct aaactgcaac tttagactta ttagctaaag ccaatattag
     1921 acctaaagct atagatgcaa atgcagaagc aattactcta tactcagcag ggaataagaa
     1981 tacaattgta tgacatggta tccagaaaaa tggtatagtt tttaaaacac taaaaccaag
     2041 gtaagcattc cagtccacaa tatttaccac atctttaagt tttaccttaa tcccctttcc
     2101 aacctcatat tttttatcta taaaacagtc cataacctta tggaataaaa atacagttgg
     2161 tgacattagt atattcatag ttgcactctt aaatagagca tttgcaaaag ctattccctc
     2221 aaatggaagc ataccatcca cttgtgcttg acctacacct gatgagtaga ttgacattcc
     2281 atatgtggta actacaccta ttattcccca aactattgct ctataaaata ctcctactgg
     2341 tattttccaa tccttataca aaattctagc accaagtaaa tctcccattg tagctaatac
     2401 agcaaacttt aaaaaacccc caatatatgg atgtgcacct gttgcttcca taaatacttc
     2461 tctactactt ggtattgcta agacagcaat aatacaaata agaactccac tccatataaa
     2521 atcgcctttt ttcatattta taaatactat ttaatatata aatagttctc ctttctatta
     2581 aagatttatt taatttacaa tctatctaca attatttatt aatcacttat tattagatat
     2641 tcattttcca tatttaaatt tattctatcc ctttatctat ttattttctt actactctat
     2701 ccaccattat ctatatattt attttacgat tactctttca ccctaaccct ttaattatct
     2761 atttttttct gatattctta tctaccatca tccatatatt tattttacga ttactctttc
     2821 accctaaccc tttaattatc tattttttct gatattctta tctaccatta tctatatatt
     2881 tattttacga ttactctttc accctaaccc tttaattatc tattttttct gatattctta
     2941 tctaccatta tctatatatt tattttacga ttactctttc accctaaccc tttaactatc
     3001 tatttttttc tggtattctt atctactatt atccgtatat ttattttatg attactcttt
     3061 taacctaccc cctatacttt atagtaatat gtttttaacc tttttcatta ttcccaatat
     3121 gctttataac tacaaatact ctcccccctt aaaaataata ataaaattaa tcaataaatt
     3181 tataatcata ttattactct atgtattaag caattactat gccaataaat tttgattttt
     3241 ttaaacaaat cttaaaaatc ctaaaatgct aattttcttt aacttttaat gaatctaata
     3301 gataatattt aattttcgtt atatttttca gaatgtttca attattattt ttaagaaaaa
     3361 aaaataatgt taacatttct taacatacat taaaaaatgt taacattata aatttggttt
     3421 aatactttta ctttgactcg atttactttt attctacttc ttactttatt tttaacttag
     3481 actttatttt tttattactt atttgagtct tatttatttt attctcactc catatgttca
     3541 attacatttg tctaaattct ttgatacttt tattacataa ttttaacaag cttaagtgtt
     3601 atttgggtag tgcagttgat aattatttaa ataatcatca actgcatgtt tatatcatta
     3661 cagtagctta atgtatttaa tcatacttac ttaggatttt gaaatatctt agagattata
     3721 tttaacatta gtcaattgct gcataattgt aagcaattga aatttgagac ccatcttcat
     3781 tttctatagt gataggcttc ataattttta atccttcagt ttttatcttt tctaattctt
     3841 gttccatttt cttaattgtt tcctcaaggt ctttttgact tacatctttg actgcattag
     3901 ggtctttagc atagtctttt aattcttgaa tatgtttagc atactcttca taagtataat
     3961 gctcaacacc atacttttcc aattcagtag ggtcagttat tcctgatttt attagagatt
     4021 caagtttagg taaagcaata ttagatgaat catttccatt tgcaaatact gctccagttc
     4081 ctcctagtac cattgcagat accatcattg cacttataag ggattttttt attttcataa
     4141 ccaatcttcc tttcttagag atttatttgg gtagtgtagt ttaaaatgac tattgcaata
     4201 gttatcaacc acatctttag actaccatag tagtcatatt tgtgtcaagt ctttttaaaa
     4261 aacttttcaa taaaaaatac tatctcaaaa caaaattaat tattttgaga tagtatttta
     4321 attctaaatc ttttatatta ctaagattta tggttactct atccatgttc tatattgttc
     4381 tttattaact gctaacaaat tcccttttct tgatatgaca aagcgattat cattatagtt
     4441 actttgctca attgaaactt ctcctgaatc agtataaagt acatccttac tattactaga
     4501 tttatttaca tacgtttctt cactattctc agaattagtt actttgctca tagatgattc
     4561 ttcatgctct ctaaattcaa ttacagatac ttttgccgta ctaaaatgag tctctccatc
     4621 acttttagct ttactaacaa gaatatcaga ttctactttg ggagaatata aatcagttga
     4681 tttactactt attgaaaatc cacttttatc actttgttca ttttttgtta aaccaaaaga
     4741 ttcaacctca ttaatctctt tattttctgt tgcaaaaaca agacctgtcc cacaaactaa
     4801 tacagttgaa actaaaaaag taacataagt tgatttttta aatttcataa tattaactat
     4861 tctcttttct atttttgtct ttccaaatcc actacatatt gtcattgaaa atggattatt
     4921 ctcctctgct agacttatta gtgtggttgc ataatcagcc ctatattctt caccaagtat
     4981 actcataacc ttttcatcac aactaaattc tatgtcttta tttagtaaaa aaaacatcac
     5041 ccaaactaag ggattaaacc aatggacaac aagagctaca agcataaata actttaacag
     5101 attatcaaag ctattaatat gaaccatttc atgagttaaa acaaagtcta aagtttgatt
     5161 attagcataa ttcatttgct ttggcaaaat aattcgtggt tttaaaatac cagatgtaag
     5221 tggtgtccta gtaaattcag atatgcaaac aataaatctt ctaaaacggc tattcttaca
     5281 catccatttt ttaatgaact catgattttt tatatgaatt gaagttttaa aaagattaat
     5341 tattttaaaa tatctaaagc ataaaagtat gctaaacata atagtaacta taaaagacaa
     5401 aatgcccaac atttccaaca tcataatatt ttcaatatta gacattattt caaattcgtc
     5461 agaattgttt ttacctgtag agtcactagc tttcttaaga gtataatttt ctgttgatgt
     5521 attacccaat tctacatcaa aagtcgattc aattatacta taaatactaa aaccagaatt
     5581 taaacttact ggtattagaa gtttaaataa aacaatcaac cacaataata caaatacctt
     5641 tttaggtaat tttttaaata aacatgctct tataaaaata actattaaaa gtaatactcc
     5701 agaagtaaaa ctcatttcta acaatttaat gaacataaat cttacctcac tttaattttt
     5761 caacaatttg tttgagtttt gtaatctcat cacttgaaag attttcttta ttaataaaag
     5821 ctgaaagaaa tttttctgaa gaccctccaa acattttatt aattaaagtt tctgtttcct
     5881 gtttttgtac ttcctctttg cttattaaag ctttacaaac aaaatttggc tcaattctct
     5941 ctattgcacc ttttgacact aattttttta taacggtata cgtagtgttt ctattccatc
     6001 ctgtttcttc ttttaaaatt tttactaact ctccagcctt gctttctcct ctctcccata
     6061 gaacttccat tactttaagt tctgaatcaa ataattttat ctccatataa aatcgctcct
     6121 caaatatagt tatcacaata gtcattacta taataaaaaa ctatcatagt agtctatact
     6181 atgtcaatca tttatttttt aataataaaa actgtctttt taaaaaattc ttataaaaga
     6241 cagttttatc aatgttatac tttaatgttt aattaaagcc atattttatt taaatgtata
     6301 actatctatc ttagtacata gtataagatt tttctttatc taatagtata tcctctacat
     6361 ctgggttttc ttttactaat tgtctataaa aattacaatt agtaacacct acatatggaa
     6421 ttagccatag tagacctatt ccaagagtag ctattgaaag tatataccat cctataaaac
     6481 taaagtttag tacaaataat ttaagtacat ttcctttcat catatctaaa ctagcaccca
     6541 ttgatttaaa tacacccata ttatccttgt cttcacaaat taagaaagta gcttgagaaa
     6601 catataatag caatatataa accggtaaca taagaacaag tattataaga actagagtta
     6661 ttccagtaga aataatagtc cctattccaa cattatcact tgataaatat tcaattgctc
     6721 cactagtaag taaaactcca aatgataaaa acgctcctat aattgtgatt ataaaagaat
     6781 aaatataaac aataattgat tcaaataaat aaaaaccaaa acttcttaaa taaattctcc
     6841 cacttacaaa gatatctcct atcttgactt tttcatcttt agtaattttc aagaacatac
     6901 atgtaaatcc tgctgaaacg caaaaagcta ttacagccac tacaatagta gtcaatggtt
     6961 caaaaggtaa tcctacaatt gatattaata ctatcaatgc aatatatact gccatagaaa
     7021 ttgcacatat tccccagttt ccagataact gagctttaga tactcgtttt aattcttgcc
     7081 ttgataccat aaaaaacacc cctttcattt tataaaaaaa cttttaaaaa tataaataat
     7141 cttttaacgt ataatattaa tatatcatat tttcgactat ataagataca attattcacc
     7201 atgttttatc aagatatgcc ataattgtat tactagtttt atatagtcaa cattaccaaa
     7261 tacatcaaa
//
LOCUS       DAEPLF010000170         7264 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEPLF010000170 DAEPLF010000000
VERSION     DAEPLF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.170"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            268..1437
                     /locus_tag="KR314_002854"
     CDS             268..1437
                     /locus_tag="KR314_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329223.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            1818..6071
                     /locus_tag="KR314_002855"
     CDS             1818..6071
                     /locus_tag="KR314_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBH0329224.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKISYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            6220..6714
                     /locus_tag="KR314_002856"
     CDS             6220..6714
                     /locus_tag="KR314_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBH0329225.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSVYIGDEKFNNLKNECIKK
                     GIDIKISKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKNIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            6830..7207
                     /locus_tag="KR314_002857"
     CDS             6830..7207
                     /locus_tag="KR314_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBH0329226.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT         2906 a          754 c         1339 g         2265 t
ORIGIN      
        1 acaagttaca acttatattt gcatattagg tcgttagaga agtatatata tatttagtaa
       61 ggaatcatac tttttataaa atgaaagata agatgtcgaa aacttgcata aatctgaaaa
      121 atttttatta ttaaaatact gattttcatt ttaaaaatga taaaaaaatg acaaataaag
      181 ttgcaaatag taatacttat aagtataatt aaaaaagaga aaaggttttg ttaaacttta
      241 taacaaaaaa agaagggatg attcaaaatg gagaagttaa cagacaggag acaggttaag
      301 gcagtaagca tggaaacctt cattttccta atattactag tagttggttt tgggtatgtt
      361 ggtagtatta tgggagcagg aatgatgttc aaagtaataa tgagtacagc acatgcttta
      421 ctattggaga cagttttctt aattatggct atggcagttc ttgcaggcgc attaagcgca
      481 ctactttcag agtttggagt aatagcgtta attaataaaa ttttcgctgt atttatgaag
      541 cctttatatg gactacctgg tgctagtata gcaggagcta taacaactta tttatcagat
      601 aatccagcta ttataccttt tgctaaagat aaaacattca cacagtattt taaacaatac
      661 caagttccag cattatgtaa cttaggaaca gcctttggta tgggacttat cttaacaaca
      721 tttatgattt ctcaaggtac agaatatgta cttccagcat taattggtaa tttaggtgca
      781 attataggta gcgtcataag tgttagaata atgttgacat ttactaagaa gttttataaa
      841 tacaatccgg aagaagataa agcaacagga acattagaga aaaaggatga atttagagaa
      901 atcagagaag gaaatgtatt tcaaagagct ttagatgcaa ttcttgaagg tggtaaaatg
      961 ggggtagata tgggaatggc tataattcct ggagtcctag tagtatgtac attggtgatg
     1021 ttattaactt ttggaccatc tacagaccct gttacaggtc aagaagttta tacaggagct
     1081 gcatatgaag gaataaaatt acttccagtt attggagata aacttggatt tatattagaa
     1141 ccattatttg gatttacatc accagaggca atagcattcc ctataacttc attgggagct
     1201 gttggtgcag caatgtctct agttccagag tttataaagt caggagctat aactcctaat
     1261 gatatagcag tatttacagc tatgggtatg tgttggagtg gatatttaag tacacatatt
     1321 ggtatgatgg atgcattaaa tgctagacag ttggcaggta aagctattct ttctcataca
     1381 ataggtggtt tatgtgcagg agcagcagct cactttatat ttacactcgt aggataaacg
     1441 taatcagcta agataaaaaa taatatacaa aaaaatggtt gttgatatgt aataagaatt
     1501 tacatatcaa caaccatttt tttctgacta aagataattt ataatacata aacattaagt
     1561 ggatattaga cttaagttat atttatgtaa aaagtaacaa aaatctgtag aaatttatga
     1621 caaaatttag tacactaact atatgttctt atttaaaatt taaggttaga attaggaatt
     1681 acctagtaga gtaagctttg attaataaaa gaatcataaa ataaaaatat tagtcataaa
     1741 gattataaat aacttactta aaataaagcc atagaactta caaaaattaa aaaagcatat
     1801 aaatatggag gggtacaatg gatgaattat ttcatatagg aattgatgta ggttctacaa
     1861 cggtgaaggt tgtggtactt aataatagta ataatatagt tcataaagaa tatagaagac
     1921 attattctga tgtaaaaaaa tcagttaaag aagtactaaa tggtatatat gaaaaattgg
     1981 gagatataaa tactaccatt ataataactg gttcaggtgg aattggaata tcaaaaaaat
     2041 taggtgtaaa atttgttcag gaagttatat ctagtacaaa atcaatagag tattttcatc
     2101 ctgaaactga tgttgtcata gaacttgggg gtgaagatgc aaagataaca tatctaagtg
     2161 gaggaataga ccaaagaatg aatggaatat gtgcaggtgg cactggagca ttcatagacc
     2221 aaatggcatc acttttaaag acagatgcca gtggattaaa tgaacttgct aaagggtata
     2281 atgttatata tcctatagct tcaagatgtg gtgtttttgc taaaacagat attcaacctc
     2341 ttataaatga tggagccaat cagactgata tagcaatgag tattttcaat gcagttgtcg
     2401 tacaaactgt gagtgtcctt tcttgtggaa gaaaaataga aggaaatgtt gcgtttttgg
     2461 gtggtccatt gtacttttta tctgaactta gagaagcatt taaaagagta ttggatttaa
     2521 aagatgaaaa cataatcttc ccagaaaatg cacaacttta tattgctatt ggagcaggat
     2581 tattatcagt agatgaagat aatatacaac taaaatctct aatagaaaaa ttggatagta
     2641 taaagaatat tgaagatgga gaagtaaatt tattagaacc tttatttaaa gataaaaatg
     2701 agtatgaaga attttcaaga agacatgaaa aagaaaaaat aagttatgtg gacataaatt
     2761 caataaaaag caattgttat ttaggaattg atgcaggctc aactacaact aaagcagcac
     2821 ttattgatga ggatggaaga cttgtatatt cttattataa tagcaatgaa ggaaatccat
     2881 taaaaacaac cattaaagtt ataaatgaag tatatgatat attaccaaaa aatattaaaa
     2941 tattgagttc aactgtaact ggatatggag aaggtcttat taaaaaagct cttaaaatcg
     3001 ataacggaga gatagagact atagcacatt acaaagctgc taaatttttc aataaagatg
     3061 tagattttat acttgatatt ggtggtcaag atatgaagtg cctaaaaata aaagatggag
     3121 taattgataa tataatatta aatgaggctt gttcatcagg ttgtggctca tttttagaga
     3181 catttgcaag ttctttatct atgacaatag aagaatttgc acatgaaggt atttattctc
     3241 aaaatcctgt agatttaggt tcaagatgta ctgtatttat gaattctaga gtaaaacaag
     3301 ctcaaaaaga aggtgcaagt gttggagata tatcagcagg gctatcttat tcagtaataa
     3361 aaaatgcttt atttaaagta ataaagatta gagatttaaa tgagattggt aataatattg
     3421 ttgtacaagg tggaaccttt tataatgatt tggtgcttag aagctttgaa aaactaattg
     3481 gaaaaaatgt aattagacct aatatttcag gaattatggg agcttttggt tcagcactta
     3541 tagctaaaga aaaatatgaa agtggataca aaacatcctt gttatctaag gaagagttga
     3601 ataatatcaa gctagaagca agtgtaacta gatgtaaagg ttgttcaaat cattgtcttc
     3661 ttacaataaa taaattttct gataatgaaa tttttatttc aggtaataga tgtgagaagg
     3721 gtgaagctat ttatggtgat aaaaaaatag gattagaaaa gaaaaaacca ataaatttat
     3781 ttaagtacaa atacaataga atttttagat acaaaccatt ggaaaaagaa gaagcaaaaa
     3841 atggggaaat agggatacca agagtattaa atatgtatga agattatcca ttttggttta
     3901 cttttttcaa tgctatgggc tttagagttg tactttcaga taggtcatca aaacagttat
     3961 atgaaactgg tataactagt attgcatctg aaacagtttg ttatccagga aaattagttc
     4021 atggacatat agaaaattta attgcaaaag gtataaaaaa tatattttat ccaagtgtaa
     4081 caaatgaaaa taaagaggat tcaaatgcag ataattatta taattgtcct gttgtaatat
     4141 catattctga agtaataaaa aacaatgtag agaatataag aaataagaac ataaattata
     4201 taaatccatt tataagtcta aatgataagg aaaaattgaa gaaaagacta tatgatgaat
     4261 tatcaagaca tttttctgat ataaaaataa ctaaagaaga agttaatcat gcagttgata
     4321 aagctactga tgaacagaat ttatttaaaa tggagataca agcagcaggt gaaagagcac
     4381 ttaaagaaat aaaagaaaga aatatgaagg ggattgtgtt atgtggaaga ccatatcata
     4441 tagacccaga aataaatcat ggaatgccag agcttataaa ttcattagat atggcagtgc
     4501 ttactgaaga ctctatatgt catttagcca atgtacaaag accacttaga gttgtagacc
     4561 aatgggtata tcattcaaga ttgtataaag cagctagttt tgtaagagat aaaaactatt
     4621 tggaacttgt tcaacttaat tcatttggct gtggattaga tgcagtaact acagaccagg
     4681 ttcaagaaat acttaatgaa aaatcgaaga tatatactat tataaagatt gacgaaggca
     4741 acaatttagg agcagctaag ataaggatga ggtccttaaa agcagctatg tttgagagag
     4801 aaagcaaaaa tatagatgta aaaaatataa aggtaaacag gatacagtat gctaaaaata
     4861 atcagataac gaacaaacat actatattag caccacaaat gtctccaata catttccaat
     4921 tccttgaaaa agctgttaac ttaagtggtt ataatataga aattctaaaa gatactgata
     4981 gtagtgtaat tgaagaaggg ttaagatatg taaataatga tgcatgttat ccagctataa
     5041 tagttgtagg tcagctaata acagcattaa aatctggcaa atatgattta aataatactt
     5101 ctgttactat aactcagact ggtggaggtt gtagagctac aaattatatt ggattcttaa
     5161 gaaaggctat atatgatgct ggatttaaag atgttcctgt cattgcactt agtgtaaatg
     5221 gaatagaaga tagtggtata atggataata tatcactaaa attaatcaat agactcttta
     5281 tgtctgttgt ttatggtgat ttgctgatga aagtactata tagagtaaga ccatatgaaa
     5341 aagttactgg aagtgcaaat gctctatatg aaaaatgggt tgatatatgc aaatcttctc
     5401 ttgttaaagc aaaaatttct gtatttacta gaaatataaa agatatagta aaagaatttg
     5461 ataatttaga gatactagat atcaaaaaac caaaagtagg tttggttgga gaaatattgg
     5521 tgaaattcca tccaattgca aataataatt tagtagatat attagagcga gaaggtgctg
     5581 aagcagtcgt gccagacctt acaaatttct tcttaagttg tgcctttaat actatataca
     5641 aacacactca tttagaagga agtagaaaga gtagaatgat aggagaagca tttatataca
     5701 taactggaat atatcaaaga gtatataaaa aagcacttga taagagtgag agattttatg
     5761 ctccagccaa tataaaaaat gttgctaaaa gtacagaacc agtagtgtca ttaggtaatc
     5821 aaactggaga aggttggctt ttaacaggtg agatggttga attattaaat gaaggtgtag
     5881 aaaacattat ttgtatgcaa ccttttggat gtttacctaa tcacataatt ggtaagggtt
     5941 ctataaaaga attaaagagg ttgtataaaa atgcaaatat aatacctatt gattatgacc
     6001 cttctgcaag tgaggtaaat caacttaaca ggataaaact tatgctttct aaggctttta
     6061 aaaatatata agatttatat tttgatatgt taaaagttaa tgaatttaaa aatatactct
     6121 atattaagta tctataaaaa agagttatcc tattttttag ggtaactctt tattagttaa
     6181 aaaattatat gtatactgat attaaagggg ggaagtaata tgagtttagt atatgatgtt
     6241 tataaatcaa aattaggaaa tttgtacata ttaagtgaag atggttttat agttagtgta
     6301 tacataggag atgaaaaatt taataattta aaaaatgaat gtattaaaaa aggaattgat
     6361 ataaagataa gtaaggaaga tttaaaggaa gcaataagcc agatagatga ttactttaat
     6421 ggaaaaagaa aagtctttaa tttaaatatt cgtattgaag ggactgaatt tcaaaaatca
     6481 gtttataggg aaatgttaaa aataccttat ggagaaacat tgtgttattc agatatagca
     6541 agaaatataa acaatccaaa atcagtaaga gcaataggac aagcaagtaa aagtaataaa
     6601 attccaataa taataccttg tcatagagta gttggcaaaa aaaatattgg tggatatatg
     6661 gggaatcatt ctgatttgaa agagatacta ttaaatttag aaagacagag ttaacttaga
     6721 aagaaatgtt tttattgtta atatttgata taataatagt aaaaagaagt attttttaca
     6781 aaaacaatga agaatttatt ttaatttaca aaaatgaggg ggaattttaa tgtgtgataa
     6841 agttatagca tcaaaatatc atgagaaggg atttaattgt gcagaatcag ttataaaagc
     6901 atataatgag gaatttaata cagatatacc agtttgttta ggaagtggac tgggttcagg
     6961 gtgtggggtt gctagtttat gtggtgcagt aaatgcatca aatataataa ttggatacgt
     7021 aaaaggcaga aaccacgaag atgaaagtac taaagctaaa gtatatgcta aagatttaac
     7081 tacaacagta agaaaagagt atggttcaga gttatgtata gacctaaaaa aagatttagt
     7141 tgcttgtaga gaaataatgg atttcgcata tgatagtcta aaggaaactt taaaaaaaga
     7201 attataagaa aataagctat caagtataaa ttatttgata gcttattttt atagtcgata
     7261 ctaa
//
LOCUS       DAEPLF010000171         7233 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEPLF010000171 DAEPLF010000000
VERSION     DAEPLF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.171"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            240..2429
                     /locus_tag="KR314_002858"
     CDS             240..2429
                     /locus_tag="KR314_002858"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBH0329227.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEEFIDIKTDLEKVTKANISIRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
     gene            2497..3264
                     /locus_tag="KR314_002859"
     CDS             2497..3264
                     /locus_tag="KR314_002859"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBH0329228.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            3322..4338
                     /locus_tag="KR314_002860"
     CDS             3322..4338
                     /locus_tag="KR314_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBH0329229.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVYAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            4404..4652
                     /locus_tag="KR314_002861"
     CDS             4404..4652
                     /locus_tag="KR314_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329230.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELGVLLEEKKELEEE"
     gene            complement(4829..5326)
                     /locus_tag="KR314_002862"
     CDS             complement(4829..5326)
                     /locus_tag="KR314_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBH0329231.1"
                     /translation="MYKIGESVMYPKEGACYISGLVTKDINHHIQKYYELTVIYNSNL
                     KISIPVLNADKIGVRPIMDENEVENFIQSLDKVDCLWVFDRKKRLKLYHDKFHSGDVF
                     EIVKLIKMLMIQDNSKQLCSTDKDFLNKAQRFALSELAAAQCKSYTIVLEEMKNHILN
                     SKNNN"
     gene            complement(5567..6631)
                     /locus_tag="KR314_002863"
     CDS             complement(5567..6631)
                     /locus_tag="KR314_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329232.1"
                     /translation="MIRLTIANIKRYIKNHALLVNMVLFPVILMFYLNFFTGNSENQS
                     MYFSPVAIVSNSSGKYEHKLIDSSKLKENSFGLNEQDKAMDLLKNNKVSAVFILDKNF
                     SSNIDNLKRPEVKCFKTENGGGSLWAESQIESFITNSLKLKIDRNIDDKLTKTNIIDN
                     SSGKNKNSFLIVFLICYFMYINAAHLASDLFSLKKSNTLKRLVSTKHNDIKIIFSIFL
                     GLFFTQAIVYTLTLVCFTSISNFKLTLNTLMIVLANSFVATGFVFWIARVFKNEGSIS
                     LMSTFYSLIGLAISISNMIPSLDKLSFMANLSKLTPFYWTIDAINNNGNILINIIVLI
                     LIGMVFVTAGSLNLRDFARN"
     gene            complement(6634..>7233)
                     /locus_tag="KR314_002864"
     CDS             complement(6634..>7233)
                     /locus_tag="KR314_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329233.1"
                     /translation="NNVKQNSYEYFALVSLGFFAIIFIMNSILSNYVSESKGLSKRLY
                     SIPVTRVQFLIYDFIGFWIYSFIFLSLYVLFFRTLGVTFKGNFVILALLCALASYFMT
                     SISTFITTFFSKKYGTVIVYALLFLQTVFGGIFSIVNDVFTKFTSLSPTYLIGTLFSN
                     YETFKTIDSIESLIFTCLIASTVLIALSIVKEKYKWREV"
BASE COUNT         2902 a          761 c         1261 g         2309 t
ORIGIN      
        1 taaagtatat aagagtataa tttgactttt atatactttt ttatattatt tttgagtttt
       61 ttaggaagat attttacaag ttaagaaaac tataaggagt gttatattga atataaaata
      121 gtataatata tattatgtta gataattaat atgaaaatat acaaatatat aaaaaagttt
      181 gtttagagtt atagttaatg tgtgtattaa tttaatacta atttattgga ggtttaaaaa
      241 tgaaatacat aataaaaaga gatggtacaa aagaactttt tagtaaaagt aagattgaag
      301 atgctgtcta taaagctagt ataaatagtg aaggtggagt agatagagag ttggcatatg
      361 atatatcaag aaaaatagct aatgactata attctatgga aaaatgctta tccgtagaag
      421 aaatacaaga tatagttgaa gtactactta tggaatcaaa tagaaaagat atagcaaaac
      481 actacatatt atatagagaa agacgctctg aaataaggac taaagcttgg gagtttgatg
      541 aattacaaaa atcaatatgg aagaacaagt atagacataa tggtgagagt tttgatgaat
      601 ggataaagag aatttcaaat aatagttcaa gaatatcaaa actaataaga caaaaaagat
      661 ttctatttgc tggaagaatt ttagcaaata gaggattaca gaaagaagga aaaaaagtaa
      721 catattctaa ttgttatgta atagaaccac caaaagatag catagaagat atatttgata
      781 ctgcaaaaaa acttgctaga acttttagtt atggtggagg agttggaatt gatatttcaa
      841 agctaagacc aaaaggagcg aaagttaaca atgctgcaaa acatacgact ggagcagtct
      901 cttttatgga actttacagt atgacaactg gcttaattgg tcaaagaggg agaagaggag
      961 ctcttatgat ttctatggat gtaaatcatc cggagataga agagtttata gatataaaaa
     1021 cagatttgga gaaggttact aaagcaaata tttccataag ggtaacagat gaatttatgc
     1081 aagctgtaag ggaaaaaaag ttatataatt gtacttttga agttgaaact actggagaaa
     1141 aaatagttaa gatagtaaat gcttatgatt tatttaataa atttgtgaaa aataactggg
     1201 attatgcaga accaggtata ttgttttggg ataaaataaa aacatataat tttatgagtg
     1261 aagataagaa cttttgcttt tcaggagtaa atccttgtgc agaagaacca cttccaagtg
     1321 gaggaagttg tctacttggc tcaataaact tgtcagagtt tataatacaa ccatttggtg
     1381 aacatgctac ttttgatatt aataaattaa aatcttgcgt tagagaagct gtagtagctt
     1441 taaatgatgt cttagatgag gggttgaact tgcatccatt acaagaacaa agagatagtg
     1501 tacataagta tagacagata ggtttaggtg taatgggtat agctgatatg cttataaaaa
     1561 tgggaataag atatggttct gatgaatctt tagaactatg tgatttaatt ggtaagacta
     1621 tgttaaatga agctgtgaag caatcagcat taattgctaa agaatatgga acatttagtg
     1681 agtataaata tgaaaatatt tctaaatcta agttttttat agaaaatcta aatgaagaca
     1741 ctaaagaaat ggttaaaaag tatgggctta gaaattcaca acttttaacc atacctccta
     1801 caggttctat atctacaatg ataggtatca gtggtggaat agaacctata tttaatcttt
     1861 catatataag aaaaactgaa agcttgcatg atgaggatgt gtattataaa gtatatactc
     1921 caattgttaa agagtatatg gacaagaaag gtattacaga tgaagaatgt ttaccagatt
     1981 tttttgttac tgctatgaat ataggatatg aagaaagaat aaacatgcaa aaagtatggc
     2041 aaaagtatat tgatgcttct atatcatcta caataaatat accttttgaa actacagttg
     2101 aagatgtttt caatatatat attaaggcat gggaggctgg tcttaaagga gtaacaatat
     2161 ttagagatgg atgtaaaagg agtggaattt taataaatga aaaaaaggaa gaaaataata
     2221 aagaagataa aaaagaaaat agtaaaaaag aatattataa aaataataaa aaagaaaaag
     2281 ataataaaga tgaaaaagcc aaagaaataa taaataaaaa ttttgaagaa gatgaaaaat
     2341 ttgtgtgccc tgaatgtgga agtgaaggaa ttgcacatac aggtggatgt agtatatgtt
     2401 taaaatgtgg ctatagtgga tgcaattaaa ctctaattaa aaaattttaa tatatttaaa
     2461 aattaaattt ttaaaaacga aagatgaggg aatggtatgc aggataaaag tgtgagggag
     2521 attaaagaaa taatagagac tttagaagta gaaaaatata tggaatatat agaactgtta
     2581 agagttgatg aaagaaaatc tgtacaaggt ctagctataa aactagcaaa aaagctggat
     2641 aatattagaa aagaagaaga aagattggaa actataaata tatttgagaa cgaaggctat
     2701 gataaaggtt atttgtatat tggaggaatt gatgaggcgg gaagagggcc acttgctgga
     2761 cctgttgtag cttctgttgt agtatttaaa aaagatacaa aaatagaagg tgtaaatgac
     2821 tctaagaaac tgagtgaggc aaaaagagat gaactttttg aagtaataaa agaagaagca
     2881 ctagattatg gaataggtat agtaaataat gaagaaatag atgaatttaa tatattaaat
     2941 gctacatata tggcaatgaa aaaggctata aattgtttaa aaaaagcacc ggattattta
     3001 ttagttgatg cagcaacaat accaggtatt gacatatctc aaaatccaat tgtgaaagga
     3061 gattcaaaat ctatatcaat agcagcagca agtatacttg ctaaagttac tagggatagt
     3121 ataatgtacc aatatgatag agtataccca gaatatgggt ttaaatctca caaaggatat
     3181 ggaactaaag aacactatga agctattgaa aaatatggca taactcctat ccacagaaaa
     3241 agttttttga aaaatatact gtaaatataa tatgatataa tgtatataat ataaattact
     3301 atagttaatg ggggattaaa gatggaatat aatgaattat taaaaagtgc tagagaaaat
     3361 ttaaatggaa gttgtaaagt ttgtaaggtt tgtaatgggg ttgcttgtgc aggtgaagtg
     3421 cctggcatgg gaggtaaagg aagtggttct tcttttatag aaaatagcaa gagtttagaa
     3481 aaagtaaaag taaatatgag agtaatacat gatgtatcaa atcctgatac atctatagag
     3541 atgtttggaa agaaaatgag tgcacctata tttgcagctc cagtaacagg aactacttta
     3601 aatatgggtg gaaaaattaa tgaaagagat tacatagaac cagtagttgc aggatgtgct
     3661 aatagtggta tttatgcaat ggttggtgat acggcagtag atgctttttt aatggaaaat
     3721 ttagatgtag tcaaaaagta taatggagct ggaattgtat ttattaaacc ttgggataat
     3781 gaaaatatta taaaaaagat aagattagct gaagaagctg gtgcatttgc agttggagtt
     3841 gatatagatg cctgtggtct agtaacttta tcattacatg gaaaaccagt acttcctaaa
     3901 aatgttaaac aaataaagga gttagtaaag tctactaaac taccatttat attgaaaggt
     3961 ataatgactg ttgaagatgc tttaatggca gtagaagcag gtgtgtatgc aatagttgta
     4021 tcaaatcatg gaggaagagt gcttgactgt acaccaggag catgtgaagt tttaccaaaa
     4081 attgctgatg ctgttaaagg taaagttaca atacttgcag atggtggagt gagaactggt
     4141 ttagacgtac taaaaatgat aggtttaggt gcagatgcag tattaatagg aagacctttt
     4201 gtgactgctt catttggtgg tgcaactgat ggtgtagaaa cttatgtaaa taaacttcaa
     4261 tcagagctaa gtagttctat gatattaact ggatgtcaaa ctataaaaga tattgatgga
     4321 aaagttatat acaaataaaa ttttatacaa aaaagaggaa aaatcatttt aatgtagaat
     4381 aattgtacaa agggggaatt tatatgagta gaaaagctta tgaagaagcc ttagtagaat
     4441 tagaaaaatt tattgatgaa agaaaagaaa taattaaaag tgcggaggac tgtattgata
     4501 agtacatagt tgacagaacg ctaccttttg attacaaaga taaatgtgtg gaatggcaac
     4561 aagagttact tgatattgca gaggcacaag ttttagaagc aaacgaatta ggtgttttac
     4621 tggaagagaa aaaagaactt gaggaagagt aagttttatt actaaatcta gggaaaatca
     4681 agaatatgat ttacccctag atttttttat ttaaataaag atgtctatat aattaatcta
     4741 tctttatcaa tgttagatta aaaggtttga agaaaaatag agtaattgta tgaatagtta
     4801 aacaattaaa ctagtctata agaccaattt aattgttatt ttttgaattt agtatatggt
     4861 ttttcatttc ttctaataca attgtgtagg atttgcattg tgctgcagcc aattctgaca
     4921 aagcaaacct ttgagcctta tttagaaagt ccttatctgt gctacatagt tgttttgaat
     4981 tatcttgtat cataagcatc ttaattaatt ttacaatctc aaaaacatct cctgaatgaa
     5041 atttatcatg gtatagttta agtcgttttt ttctatcaaa aacccataaa caatcaacct
     5101 tatcaaggga ttggataaag ttttcaactt cattttcatc cataattgga cgtacaccaa
     5161 tcttatcagc atttaaaact ggaatcgaaa ttttaagatt actattgtaa ataacagtca
     5221 attcatagta tttctgtata tgatgattta tatcctttgt tacaagacca cttatataac
     5281 aggctccttc ttttggatac attacacttt caccgatttt atacatatgg ttatacctcc
     5341 aaactgaatt ttagatattg caatttgggt acaatgcttt aattatctaa taattaatat
     5401 tatatcacga aatgcttgac aagtcaaaaa gattaatata taataaaaag tttagaataa
     5461 tagatatata taattggcta taaaaatata agataagagg atttaatata ttaaaatatc
     5521 tagttagtat tgtataaaat aacatactaa ctagatttaa taattattaa tttctagcaa
     5581 aatctcttaa gttaagactt ccggctgtta caaataccat tccaataaga attaaaacaa
     5641 ttatatttat taatatatta ccattattat taatagcatc tatagtccaa taaaatggtg
     5701 taagctttga aagattagcc ataaaggata atttatcaag tgatggtatc atattagata
     5761 tggatatagc taaacctatt aaagaataaa acgtagacat aagacttata gaaccttcgt
     5821 ttttaaacac cctagctatc caaaatacaa atccagtagc aacaaaacta tttgctagta
     5881 caatcatcaa tgtatttaga gtcaatttaa aattacttat agatgtgaaa catactaaag
     5941 ttaaggtgta tacaattgct tgagtaaaaa ataaccctaa aaatatgctg aaaattattt
     6001 ttatatcatt atgctttgta gaaacaagtc tttttaaggt attagatttc tttaatgaaa
     6061 ataaatcaga agctagatgt gctgcattaa tgtacatgaa ataacatatc aagaaaacta
     6121 ttaaaaatga atttttattc ttacctgaac tgttatcaat tatatttgtc ttggtcaatt
     6181 tatcatctat atttctatct atttttaatt ttaatgaatt agtaataaaa gattctattt
     6241 gtgattctgc ccaaagacta cctccaccat tttcagtttt aaaacattta acctcaggtc
     6301 gttttaagtt atctatattg cttgaaaagt ttttatctaa aatgaagaca gcagaaactt
     6361 tattattttt taatagatcc atagccttgt cttgttcgtt taatccaaat gagttttctt
     6421 ttaatttaga actatctatt aacttatgtt cgtattttcc agaagaattg gatacaatcg
     6481 ctactggaga aaagtacatt gattgatttt ctgagttgcc agtaaaaaag ttaagataaa
     6541 acattaatat aacaggaaac aaaaccatat taactaaaag agcatgattt tttatatatc
     6601 tttttatgtt tgcaatagtc aacctaatca ttattaaacc tccctccatt tatatttttc
     6661 ttttacaata gaaagtgcaa tcaatactgt agatgcaatt aggcatgtaa aaattaaact
     6721 ttctattgaa tctatagttt taaacgtctc ataattactg aacaatgttc ctattaaata
     6781 agtaggagat aaacttgtaa acttagtaaa cacatcattt actatgctga aaatacctcc
     6841 aaaaactgtt tgcaagaaga gtaatgcata aactataact gtaccatatt tcttgctaaa
     6901 aaaggtagtt ataaaagtag atattgaagt cataaaataa gaagctaaag cacaaagtaa
     6961 agctaatata acaaagttac ctttgaatgt tacaccgagt gttctaaaaa ataaaacgta
     7021 taatgataaa aatataaatg agtaaatcca gaaacctata aaatcataaa ttaaaaattg
     7081 tactctagtt acaggtatag agtagagtct ttttgaaagt ccttttgatt cacttacata
     7141 atttgataaa atgctattca ttataaaaat tattgcgaag aatccaaggg aaactaaagc
     7201 aaagtactca taagagtttt gtttaacatt att
//
LOCUS       DAEPLF010000172         7142 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEPLF010000172 DAEPLF010000000
VERSION     DAEPLF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.172"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            48..1451
                     /locus_tag="KR314_002865"
     CDS             48..1451
                     /locus_tag="KR314_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0329234.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIIFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLTLANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGSK
                     FVGGKSIVFSIIGIIFLIILISGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNKYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            1671..4148
                     /locus_tag="KR314_002866"
     CDS             1671..4148
                     /locus_tag="KR314_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0329235.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDVLTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEMTENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIENVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDVVMIQIRDLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKKRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            4163..4849
                     /locus_tag="KR314_002867"
     CDS             4163..4849
                     /locus_tag="KR314_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329236.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALVGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            5122..5955
                     /locus_tag="KR314_002868"
     CDS             5122..5955
                     /locus_tag="KR314_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329237.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCMQLKNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMYICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            5993..6454
                     /locus_tag="KR314_002869"
     CDS             5993..6454
                     /locus_tag="KR314_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBH0329238.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            6607..7032
                     /locus_tag="KR314_002870"
     CDS             6607..7032
                     /locus_tag="KR314_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0329239.1"
                     /translation="MHDELKIKMYSFTMDCKDPHELAKFYAALLNWEIAFYNEEYACV
                     GAPGTNQGTYPGITFQRNSEYKPPVWPEKPESQQQMAHMDFAVNDLEEAVQYEIHCGA
                     TIAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
BASE COUNT         2757 a          785 c         1124 g         2476 t
ORIGIN      
        1 aagcaacaat caaactaaaa taaaagttta agaaaagagt tgattaaatg cataaaccta
       61 gttataaaac agtatttcat tcatacatta tttttttcat gatttttatt tgcttaatcc
      121 tgacagtaat aggtattttc ttatcactta ttactattca gaaaccaaat ggggaattta
      181 ttcgtagtga ctggccgaaa aaatttacag aaaactttaa tgaacaaatc atatttatag
      241 ataataagcc acaagtaaaa cagtctggat tagaaaagct tcgagaaaat caattgtggt
      301 tgcaaatttt agattctagt ggaaatagag tttatgggtt tttagaacca aaagagcata
      361 aagattatta ttctaattct gaattattaa cattagctaa ccaaaataat tcaagtaatg
      421 gaactgtata ctttaataca attaaaaata ataaaaatga ctatatttat attatatatt
      481 ttccattaaa tatatctaaa gtgaatatgt tttttgatgg aagtaaattt gtaggtggta
      541 agtcaattgt attttcaatc atagggatta tatttttaat aattttgatt tctggaattg
      601 cttatggtta ctgggtaaca aagtgcatat caaatattac atctgctgtt agtgatattt
      661 caaaacgaag ctatcttcca gttaatatta aaggtgtttt tgctgatata tataatagtt
      721 taaatatttt aaatgaagaa attcatagaa gtgatgagat acaaaataaa actgatatta
      781 tgagagaaga gtggatttca aatataactc atgatttaaa aactccactt tcaccaataa
      841 aagggtatgc agagcttctt acagatagta gtactaatta ttcacctgaa aaagtatata
      901 aatatgcttc aatcatgtta aagaatatag aatatacaaa tacacttata aacgatttaa
      961 aattaaccta tcaattaaaa aatggaataa ttcctttaaa taaaaataaa tatgatttag
     1021 taagattctt gaaagaatta tccattgata ttttaaatta tccagaatat gaatcaaata
     1081 tagttgaatt taagagtaat atagataata ttaactttga ttttgattgt acactattaa
     1141 aacgagcttt tagtaatctt attgtcaatg cttttacata tggagataaa aatactaaag
     1201 tttgtgtaca tataacatta aatgaaagaa ttgaaatcat tatatctgat aatggaaaag
     1261 ggatgtcaag agaagaagca tcaaatttgt ttaatcgtta ttatagagga agcaatacaa
     1321 aacaaaaaat agaaggaact ggtttaggac tagcaataac gaagcaaatt attgaagttc
     1381 atggagggaa tatatctgtt gaaagtatac ctggattagg tagctcattt tatatcacct
     1441 ttcctattta attaaggtta attaaggtca aataaaaaat atattaaggt tgatagatta
     1501 ttatatttat atagtagttt atcaatcttt tttatttaag gaggtgattt gattaaaaat
     1561 attataaata gaatgataaa agtatggagg agtctattag agattcaatt tggattaaag
     1621 tataaaaata ttatactgaa atatcttaga taaaggaaga aggtgtaaat ttgaaaatta
     1681 tattaaagta tattttcaca aatattaaag aacgaaaaat tagaacagca gttatgttac
     1741 tgtccatagt tttatcaaca gttttacttt ttgtttcatt ctcaattgga ttatcttatg
     1801 aaagtgcaca aagaaaaatg gcaaaaggta tgtatggtac agcgactata tctgtccaaa
     1861 gtaaaaatcc agatgtatta accaatttag aagacatacc tgatttaaat gctataaaat
     1921 caaaggttgg tgtattggaa agctctgcta tttataataa agggggatac tatgaagaat
     1981 ttagtcttat atcagcagac ttaagtcaac taaataaaat caacaagcca cgattagaaa
     2041 atggcgatag tattactgat ttttctggtg ataaaattat tcttccaaat cgctttacat
     2101 caaaatataa aataaaaaaa ggtgattcta ttactttaca gatttatgga aagtcttata
     2161 cttttcaagt tagtgatatt gcatcttatg atacagtatt tttgaggaat acaagaggtg
     2221 taaatgcact gctacccaaa gagactctat caaaaattat aaataaaggc agtggatata
     2281 caagagttct tatagagtca gaagaagaaa tgacagaaaa tctagtaaat aaattatccg
     2341 aagaactttc cactgaaaaa tatatggtat caaatactat aaatgaaact aagataattt
     2401 cagatgctag acaaaagaca atgcctttct ttttaataag tttttttgca ttaaccctta
     2461 gtatatttat catatacagc agctataagg ttattacatt agaacgtctt ccttttattg
     2521 gaacatttag aagtattggt gccaatgaaa aaactgtaac tcgtatattg atgcttgaaa
     2581 gtatattata cggaagtata ggaggattaa ttgctattcc tattggtgta gttgttttaa
     2641 atttaatgct tcatgggtta ggaaattctc tcgaacaagg aatatcaatt ccagtagtaa
     2701 tatcacccat tggagtgatt atatctgtta tagtagcaat aatcgtttct tcatttagtg
     2761 catatatacc tgttaaaaaa gctagccatt tgccaataaa aaatattgta ctaggaacag
     2821 tagaagaaaa gaatgtttca aatcgttcta tactttttat aggaagtata atgtttattt
     2881 tatctatttt acttccaaga atatcccctg aaaatacatt gtatctagct ggtggatttt
     2941 cattactagg attaattgta gctactattg tgttgattcc tcttattact gatatcatgt
     3001 ctatagtttt tgagtttgtt tataaaaata ttttagggaa tgagggtaaa ttagctgcta
     3061 gaaatatgaa aaataacaaa aatataatac agaatatcac tttactattt ataagtattt
     3121 ctgcagttat tgcaataagt gttgttggta actttgtcaa aacttatata acagatgtgt
     3181 ttcgtgacgc agaattacaa ggttttgcag atggaaagat gaatgaagag tttattgaaa
     3241 atgttagaca tatggatgga attaaaaaaa tattacctct ttatgttatg aataatgaga
     3301 tttctggtaa tggtgtaaca ttatcaagac tagagggtac agataatata aaactatata
     3361 actctatgtt tggaatcaat tatacaaatt ttgagataaa aaaacaagta atagaagctt
     3421 ttaatgataa acgttctgtt attttgaatg aagatactct aaaaaaagta ggcttatcta
     3481 taggagatac aataactcta tctaatgata agtatgattt ttcttataaa atagtaggta
     3541 gttttaaatc ccgtgcaaat gatgtagaag ctgttatacc atcacattat gcagttaatg
     3601 attttgataa aactaattat ggttttttag tatatacagc agtcaatcca gatgttgtta
     3661 tgattcagat aagagattta tttggagata catataattg gagtcgtaca gttgaagaat
     3721 ttaataatga ttctttaaat actataagta gttttttgtc tcctatgaat aagatgacct
     3781 atttcatttt tcttcttgct acagtaggta ttattaataa tctactgatt aattatattc
     3841 aaaaaaaacg ttctatagct atgtataaat ctataggact aagtgataaa caaaatataa
     3901 aagtgacatt gattgaaggg tttacttcag gtttacttgg agctgttatt ggtattgtga
     3961 tatctatact tgagattcaa actatcttta tagttgcagg accaaaaatt tcaatgaaac
     4021 cagatttaga ttttaaaaca tttattattg ttgggttatt gggaattata gtaactttaa
     4081 taggttcgat agtgcctatt ataaaaggga agaaaatgaa actgatagaa gaaattaagt
     4141 ttgaataatt atggaggtta atatgaaaat tacacataat agtttagctg tagaagcaaa
     4201 aaatattatc aaagaatata aaattggaaa tacaacaact cgagtgttaa aagaggtatc
     4261 tttacaagta atgaaaggtg aatttgtttc tatcatgggt caatctggtt ctggtaaaag
     4321 tactctttta tatattttag gtggtttaga tactccaacg agtggaaaag tgtatatgaa
     4381 tggagctgat atatcacatt ttaatgatga gaaaatgagc ataatacgtc gtagaaatat
     4441 aggattcgta tttcaatttt ataacttaat accaaattta aatgtggagg aaaatataat
     4501 gcttcctttg ttattagatg gtaaaaattt aaaagattat aaaaatcaat tagatgaaat
     4561 cttagatata gttggtttaa ctgatagacg taaacataca ccacgagagt tatctggtgg
     4621 tcagcaacaa cgtgttgcta tagctcgagc tttagttgga aaaccagaga ttttatttgc
     4681 tgatgagcct actggaaatt tagatagtaa aacagggata gaaataatag atttactgaa
     4741 taaaataaac agagacaatg gacaaactat aattatggta actcactcac cagaagcagc
     4801 caaaagtagt agtcgtacta ttacagtttc agatggttta attgtataag gactaattat
     4861 tgtatttgag ataagcattt ttaactatat aaattagaca tatgtaaagt ggatataagt
     4921 taaatactat aaatgtgcta taatattaaa ataacagata aaagtactat attttttatt
     4981 gattatttta atttatttgt ttattaaata tctaatgagt attcatacta tctctattat
     5041 taaaagcagt taatgatata tagagataat atggattctt cttactttaa taaatattca
     5101 cgataggaca ggatataata tatgttaaaa aaagaaagta ttcattttat aaataaaaat
     5161 aggtcactta tagtttgtga agacaatttt gtttatctct tgccttgtat gcaattaaaa
     5221 aattggatat cgaattttac tatatcgttt ccaaatcaaa caataatttc tgataattac
     5281 acgattattc ctcatggaag tgtaactttg gtgttttttt atgatgccac agggttacat
     5341 agtctattat ttggtccaac tactaaacca aaaacagtag gaaatattgc taatagatgt
     5401 gatgttatac taattataga atttcaacct gctggttttt tcccgcttat aggtatacag
     5461 caaagtgaac ttattgataa agttgttcca ttttctataa ttaatacttc attagattta
     5521 gaaattaaaa aaatatttaa tgaatcttta tctatagata aattgatttt aaagttggaa
     5581 gaattattga tttctaatat taaaattgaa tactcatatg agtttatttt ggcaattcag
     5641 ttgattattc aaaactctgg aaacatatct tcacaagaaa tttctaaaaa agtattttat
     5701 agttctaggc atttaaacag actctttaat cagtacctag ggttgagtat gaagtcattt
     5761 tctcgtttgg tacgtattaa taaatctata aaacttctaa acaataataa aacttcattg
     5821 atgtatatat gtaatgaatt aggattctat gatataccac attttattaa agattttaaa
     5881 attgtctgtg gaataactcc tcaaaaatac cgagctaata tgtctgattt ttacagtgaa
     5941 attgcaaaat tttaagttat aatacttata acaaagaaag gatagtgata agatgaaatt
     6001 tggaggagta ttgctaattg tcaaagacat aaataaatca aaaaagtttt atgaagatgt
     6061 tatggagcaa aaaattacat tagatttagg agaacatgta tcatttgaaa atggatttgg
     6121 attacaaagt aattataaag ctataattgg atatgattta ccaatatatc aacaatcaaa
     6181 taactttcag ttgtattttg aggttaatga tatcaaacaa tgggaaagta agataaatag
     6241 aataggaaat attgaatttt tgcattatat aaaagaatac ccatggggtc aaagaacttt
     6301 taggttttat gattttgata aaaatattat agaaatttca gaaagtatgg aaagcgtcat
     6361 aaagaggctt ttaaaacaag gtttagcttt agaagaaatt tcaaaacgca ctatgtatcc
     6421 agttgaattt attacacaat ttcaacaatt ataaaatatt aaaatatata attttttctt
     6481 tatattgcaa aaaacaaagg cttggggcat aatagaaaaa tacttgaaat aaattacata
     6541 cttaatgttt gaatttagct taaaaatgaa aagacgaaga aatttatact tggaggtaat
     6601 tacgttatgc atgatgaact gaaaatcaaa atgtactcat ttacgatgga ttgcaaagac
     6661 ccacatgaat tagcaaaatt ttatgcagcg ttactcaatt gggaaatagc attttataat
     6721 gaagaatatg catgtgttgg tgctccagga acaaatcagg gaacatatcc tggtataaca
     6781 tttcaacgaa attctgagta taaaccgcct gtgtggccag aaaaacctga atctcaacag
     6841 caaatggctc atatggattt cgctgttaat gatttagaag aagcagttca atatgaaatt
     6901 cattgtggag ctacaatcgc agaggagcaa tttactgatg actggagagt tatgattgac
     6961 ccagctggac accctttttg cttatgtcag atgaaatcaa ttatggaaag tagccatttt
     7021 tcattgttat agaccaaaat attgtttatt aattacaaaa aagcacaaca gtatataccg
     7081 ttgtgctttt acttattata catgaataca ctaaaaaata taaatagtag agctttatca
     7141 at
//
LOCUS       DAEPLF010000173         7076 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEPLF010000173 DAEPLF010000000
VERSION     DAEPLF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.173"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..1162
                     /locus_tag="KR314_002871"
     CDS             <1..1162
                     /locus_tag="KR314_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329240.1"
                     /translation="MPVIFDIIIKNKKILFNGNNIIAINNINYRLNKNYKTYAIIAII
                     ITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVNKKQIENIIKDTNKIKYS
                     VNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKELKEYGERLVSGNNVIEL
                     KSPQTIASIATKEVKTKLDGQLYNISRGEVKVPVLGTGLNQSIIVVNDDTYFKLRDKG
                     QQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQGESQWMKFAYVVLVFLF
                     LVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINRAILKEVAIFYTLPMLSA
                     TISSYFALRLAGDLLMNDLSGIYILSLVICLIIFVIYGAVSVNKFKSVVYKN"
     gene            1314..1988
                     /locus_tag="KR314_002872"
     CDS             1314..1988
                     /locus_tag="KR314_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0329241.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFMDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIDILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYII"
     gene            1988..3007
                     /locus_tag="KR314_002873"
     CDS             1988..3007
                     /locus_tag="KR314_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0329242.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDILKNRYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYINLYNCIENSGEIDSYLIDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDDYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDY
                     LSLDS"
     gene            3437..4597
                     /locus_tag="KR314_002874"
     CDS             3437..4597
                     /locus_tag="KR314_002874"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBH0329243.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAIIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDKNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGVHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            4599..4988
                     /locus_tag="KR314_002875"
     CDS             4599..4988
                     /locus_tag="KR314_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329244.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSNKNTKKEMEKNQRTDYNIPTISFK
                     SGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRGFSL
                     GFFRLYILFVLSFAIIGYFWIYKGIHI"
     gene            5249..5557
                     /locus_tag="KR314_002876"
     CDS             5249..5557
                     /locus_tag="KR314_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0329245.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEASVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            5646..6992
                     /locus_tag="KR314_002877"
     CDS             5646..6992
                     /locus_tag="KR314_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0329246.1"
                     /translation="MLDKFTEFLDSKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
BASE COUNT         2783 a          693 c         1240 g         2360 t
ORIGIN      
        1 tatgcctgtt atatttgata ttataatcaa aaataaaaaa atattattta atggaaataa
       61 tataattgcg ataaacaata taaactaccg attaaataaa aactataaaa cttatgctat
      121 aatagctata attataacta caacaatatc aactcttggt gcttcagttg ctatgaagca
      181 tatacataag aatgcccaag aacaaagaaa tgtgtacact attagcatag tttcaatgga
      241 taaagatgaa gtgaataaaa aacaaataga gaacataata aaagatacta ataagataaa
      301 gtactccgta aatccagaac taacaatctt aaagaaaaca gatataaaag aagcttatga
      361 tacaacagat actataatga aatatagtga tttttgtaat actcttaaag tgaatggcaa
      421 tgataaagaa ttaaaagagt atggtgaaag attagttagt ggaaataatg taatagagct
      481 aaaaagtcct caaacgatag catcaatagc tacaaaagaa gtcaagacaa aacttgatgg
      541 tcagttatat aatatttcaa ggggagaagt aaaagttcca gtattaggga caggacttaa
      601 tcaaagtatt atagtggtaa atgatgatac atattttaaa ctaagagata agggtcaaca
      661 agtatatttt tatggtgcaa aggtatttca agaagaaaat agccaagtaa tgttcaacaa
      721 tattgaaaag aatttaagtt taaaaaatgg atatattcag aatggatata aagctcaagg
      781 tgagagtcaa tggatgaagt ttgcatatgt agtgcttgtg tttttattct tagtctttgc
      841 aattgtagct ggaagtataa tatatatgaa aatatatagt gatgcatatg aagataaaga
      901 gaaatacaag attttattaa aaataggtac tacagaaaaa gatataaata gagctatact
      961 taaagaagta gctatatttt atactttgcc aatgttaagt gcaactatat ctagttattt
     1021 tgcattgaga ttagcaggag atttattgat gaacgattta tctggtatat atatactaag
     1081 tttagtgata tgtttgataa tattcgtaat atatggagca gtatctgtta ataagtttaa
     1141 gagtgtagtg tataaaaatt agttgatata ttaataaaga gaaaggagat tcttgtaata
     1201 agagtctcct tgttgattaa ctaaataatt tgggtaataa atttatataa aatatgttat
     1261 gatattaata attcatggca tagtgaaata ttaggataat ggagaatata caaatgtata
     1321 gaattttatt agtagaggac gatattgatt tatcaaaaga gattgcgtta gcacttgaaa
     1381 aatggggatt taaagtagaa ttaattgatg attttgaggt agtattagat gaatttatgg
     1441 ataaaaaacc agatgtagta cttttagatg taaatttacc attatataat ggattttact
     1501 ggtgcgaaaa aataagagct atttcaaatg taccattaat atttttatca tcaagagatt
     1561 ctgatatgga tttaataatg ggtataaata atggagcaga tgattatatt accaaaccat
     1621 tttctataga catattggtt accaaaataa atggtataat aagaagagtc tataattata
     1681 gtgattctaa tagtattttg tattgtgaag atttgatgtt tgatgtgggg aaagggatta
     1741 taaaacataa gtataaggat aaaagtatag agttgactaa aaacgaaata aaaattttaa
     1801 cattattatt aaaaaataag aatagggttg tatcaaggga aagtcttatg atgactctat
     1861 gggataatga tgagtttgtg acagacaatg cacttactgt aaatatgaat agattgagaa
     1921 gtaaagttaa agaactaggt tttgatgatt ttataaaaac taagaagggg ataggatata
     1981 taatataatg ctagtaaaat atttaaaagg taggtctaag tatattgctc tacctttagt
     2041 tgtagttagt ataataaata tctatttatt tgctatagat attttaaaaa atagatattc
     2101 agaattaata tacttagatt ttttagtgct gtttataata atggtatttt ttgttataga
     2161 ttatataaat tttagaaata gttatataaa tttatataat tgtatagaaa atagtggtga
     2221 aatagacagt tatttaattg atggacaatc ttttgaagaa aatctaataa aagatattat
     2281 agaaaactta aaaataaaaa ataatagtga catagagact tataaacaat cattaaaaga
     2341 attagatgat tacatagcta aatgggttca tgaaataaaa attccaatat cttctttaag
     2401 tataattaca gataggttaa gtagcataga agatagtttg gatattaaga atcaggttgc
     2461 aaagataaat tttttagtaa actctatttt gtatagtagt agaagcaaca ctatgtttga
     2521 agatgtattt ataaataaat ttaatttaga aaaattggta aaaatgtcta taaaaaataa
     2581 ttcatttttg cttataaaaa ataatgtaga agttagtttg aatgaattag aaaatgatgt
     2641 ttacacagat tctaaatgca tgtcatatgt tttagaccaa attattaata atgctataaa
     2701 gtattcaaag gaagttggta agattgaatt taattcaaaa aagttagaaa atggagtggt
     2761 attatctata aaagattttg gtataggtat aaatgaggaa gatatatcta gggtttttga
     2821 caagggattt acggggaaaa atggaagaaa tcaattgtat aagtctactg gaatggggat
     2881 gtattttgta aagaaaatga tagatagttt agggcatgag attgaggtat gttcgaaaat
     2941 tggttcatat acaattttta atatctactt ttatgatata tcggattatt taagcctaga
     3001 tagttaggtt attagactat aaaatctatc atcatataca aatcgacata tacaataaga
     3061 ataaatttag ttcttgaaaa tagtgaagat ttaattagtg ccttaaatga tgttctatct
     3121 atattataaa ataataatta ttatatatga aaaacatatc agataggtac ctggtatgtt
     3181 ttttatttgt agtaactatt ttaatatggt ttttggagag agaatatgtt ttgtttgtga
     3241 aaaaaaatta catgcattac attgacactg aaaataaatt aatgtaagat ttagctaagg
     3301 taaaaaacgt gtataagatt aactcgatta atttcgtaag agtaaattat cgcaattaaa
     3361 gatgaaataa gattaattct aaaattatta aacttaatac cttgatttgg ggaggaataa
     3421 ctataaactt aaaaggatga taactatgta tagtgtaggt ttaatgtcag gaacttcctt
     3481 agatggagtt gatgcagtac tagctgaaat tagtgggaat ggtagaaata ctaaagttaa
     3541 gcaaatagaa tttataactc tagaaattcc taaagatata aaagacgaaa taagaaagtg
     3601 ctgtatagaa gaggaatcga gtgttgattt aatctgtagc ttaaatttta agcttggata
     3661 tttatttagt aaagctgtaa aatcagtgtg tcataaagca aactttcata tagcaaattt
     3721 ggattttatc gcatctcatg gtcaaacaat ctttcatata ccaagaagtt ataataattt
     3781 tgtatcttca acgcttcaaa taggtgaacc agcaattata gcatatgaaa caaatacaaa
     3841 ggtaatatct aattttagag taatggatat agcagcaggt ggagaaggtg ctccattagt
     3901 accatatagt gaatttttat tatatagtga taaaaataaa aatttagcac ttcaaaatat
     3961 tggtggaata ggaaatatta ctataatacc taaatcatgt aacatagatg atgtatttgc
     4021 atttgataca ggaccaggaa atatgataat agatggagta tgtcaaagat tatttaatag
     4081 aaagtatgat aaaaatggat actttgcatc aaaaggtaaa attaatgaag aaatgttaaa
     4141 agatttgatg agtcataagt atcttagtca agcgccacct aaaagcactg gaagagaggt
     4201 atttggacaa gtctatttgg ataacatgct taacaaatat aaacatgtag ataaatatga
     4261 tttgatagca acagtaacta tgtttacggc aaaagctata tattataatt ataaaaactt
     4321 catacttcca aaggttaatg tagatacttt attaattgga ggtggaggag ttcataatct
     4381 aactttaata ggatatataa aagagttatt attagaggtt gaagtattaa ctcaagatga
     4441 atatgggtat tcttctgatg caaaggaggc tttggcattt gtaatcttgg gcaatgaaac
     4501 attaaataat tctttttcta atgtaatatc tgcaacaggg gctaaaaata aagttatttt
     4561 agggaatata acacctaaac cttttggagg taaatgacat gtctaataat aaaaaaaaga
     4621 aaagaaagaa aaataataat tataaaaatg caaaaagtaa taaaaatact aagaaagaaa
     4681 tggagaaaaa tcaaagaaca gattataata ttccaactat aagttttaaa agtggattaa
     4741 tactaatagt ttcatctcta gtaagtactg tagtttttcc tttgttatta tctatatttg
     4801 gaatagataa tagatttgga gttgttatag gaaattcatt aattacatct tttgcagttg
     4861 catatacaag atactttgtt gaaactaaga gaggtttttc tttgggattt tttagactgt
     4921 atattttatt tgttttatca tttgcaataa taggttactt ctggatatat aaaggaatac
     4981 atatttaaaa aagtattgag actattttat gttaataaaa gatagtcttt tttctttttt
     5041 aagaaaagat atgagtattt taagtagtgt gtaattttag acaaaagaat gattagataa
     5101 ataaagcaat taaagcaaga tataaatgca tgaaataaaa ttacaattaa aaatgtaaaa
     5161 tatgaaaaaa tatatacaaa cgtattcctg taatcaaaaa tacatgctat aatgattgaa
     5221 aagaaataaa aaaacagggg gaatcaatat gagagtatta tttgtttgtt catcagggat
     5281 gtcaagtgct atagcagtta atgcattaca aaaagaagga gcaaaaaatg gaatagatat
     5341 agatgttttg gctgttggta ctcaagagtt tgagaatgaa gttaaaaatg gatgggatat
     5401 tgctatggta gctccacaag tcagacatag atttgactat ttaaaagctt ttgctgatga
     5461 ggctagtgta ccatgtgcat taatacaagc acaagcttat agtccattag gaggaccaaa
     5521 gttattaaaa caagttcaag agttgttgag taaatagtag ttgcagtgtt gttaggttag
     5581 aagaaagtta attttataaa aattttaatg ataataaaaa taaatattaa ttaaggagag
     5641 aaaagatgct agataaattt acagaatttt tagacagtaa attatcaact tctatggcca
     5701 gattggcaga acaacgtcac ttaagagctg taagagatgg aatcgtagct tcattaccac
     5761 ttataatagt tgggtcattt ttcttgatat tagcatttcc tccattacca gattcatggg
     5821 gggtaactca gtatctaaag gaaaatgcag ttacaatatt actaccatat agaatgacat
     5881 tttttattat atcagtatat tctacatttg gcataggtta tagtttggca aagtcatata
     5941 agttagatgg attgacagga ggtttattag ctacagctac attccttttg acaatagttc
     6001 ctactgcaat agagggtctt acccctgatt tattaaagat agttgaaaag agtccagaac
     6061 ttttagcttg gtataattca attcctcaag gatggcaaat gccaatggct aatcttggtg
     6121 gaggttcaat gtttgttgct attgtaacat ctataatagg tgttgagatt tatagattta
     6181 ctgacaaatc taaattcaag ataaccatgc cagaagcagt tccagcatca gtcacaaggt
     6241 cttttgaagc tctgacacca gccgcattaa taataatttt aatggctatt gttacttatt
     6301 tcctagggtt tgattggcat ggcttcatag ctaaattagt aactccatta gtttcagcca
     6361 ctgacacatt accatcagta attatactaa tatttttaat aacgtttttt tggtcatttg
     6421 gaatacatgg tgtatcaata ataggttcat tggcaagacc tttatggata caattattag
     6481 atgctaatac agcttcatat gctgcgggtc aagcagttcc aggtattgct gctgaaccct
     6541 tttttcaatg gtttatatgg attggaggtt ctggagctac aataggctta gctttacttc
     6601 ttgcatttag agcaaaatca gcttatggaa agtctttagg aaaagcaact gtaataccag
     6661 caatattcaa tataaatgaa ccaataatat ttggagcacc aatagtatta aacccaatat
     6721 taataatacc atttgttgtg gcaccaatag taatggcaac agtttcatgg tttgctatga
     6781 gtgctaattt agtatccaga gtagtagtta ctgctccttg gacattacca ggaccagtag
     6841 gggcatattt agccactggt ggagattgga gagcttcagt tcttaactta ttatgtatag
     6901 ctatatcaat aataatttac tatccatttg tcaagatgta cgataagaga ttgcttcatg
     6961 aagaaaaagt tgaagagcaa gaagcttatt aattagaaac tatattggaa attaaaaact
     7021 atattagatt agtataaatt tcaaagtata acagttatga aataagtaac gaagat
//
LOCUS       DAEPLF010000174         7005 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEPLF010000174 DAEPLF010000000
VERSION     DAEPLF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.174"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..538
                     /locus_tag="KR314_002878"
     CDS             <1..538
                     /locus_tag="KR314_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBH0329247.1"
                     /translation="CDVICLQEVLYPQFLALKAVLNMDGVFATNVKKVNMIYGICTFT
                     TSKMLNSNHFFLTSKKEQRGALCITIDAYGRIINVINTHLGLDRQERAKQLDEIIDYR
                     NRLVGIVILCGDFNEKNVFLSMFNDMAISLNKSYLPTFEKSNSRIDYIFVNKNTELKG
                     YTVEKIYLSDHYPVIGYI"
     gene            777..2036
                     /locus_tag="KR314_002879"
     CDS             777..2036
                     /locus_tag="KR314_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBH0329248.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLTYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            2295..3290
                     /locus_tag="KR314_002880"
     CDS             2295..3290
                     /locus_tag="KR314_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBH0329249.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRRNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            3536..4603
                     /locus_tag="KR314_002881"
     CDS             3536..4603
                     /locus_tag="KR314_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBH0329250.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHTGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSEDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            5140..>7005
                     /locus_tag="KR314_002882"
     CDS             5140..>7005
                     /locus_tag="KR314_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBH0329251.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILSYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKK"
BASE COUNT         2947 a          662 c         1165 g         2231 t
ORIGIN      
        1 ttgtgatgta atttgtttgc aagaagtatt atatcctcaa tttttagctt taaaagctgt
       61 tttaaatatg gatggtgtct ttgctacaaa tgtaaaaaaa gtaaatatga tatatggtat
      121 ttgtacattt acaacatcta aaatgttaaa cagtaatcat tttttcttaa ctagcaaaaa
      181 agaacaacgg ggagctcttt gtataactat agatgcatat ggaagaatta taaatgtaat
      241 aaatactcac ttgggacttg ataggcagga gagagcaaaa caattagacg aaataataga
      301 ctataggaac agattagtgg ggatagttat tttatgtggt gattttaatg agaaaaatgt
      361 ctttttaagt atgtttaatg atatggctat ttcactaaac aagtcttact taccaacatt
      421 tgaaaaatca aactctagaa tagattacat ttttgttaat aaaaatactg aattaaaagg
      481 atacacagta gaaaaaattt acctgtcaga ccattatcct gtaataggtt acatttaaat
      541 ttcaatataa atgaaattag ttgttataaa ataaacaatt ttttgttaag aaatagtaaa
      601 agaaatataa taaaaaatat caaaaataaa actaattata agacataaat ttacaataaa
      661 gttacatttg attaaattcc ttgtttaaca aaagctattc aaatataatc taaggtagtt
      721 ttgaaatggg aactctatta tgcaaattta agctagtgtc aaaatgaggt gaagttatgg
      781 gcagaagaac aacaagagtt tcaagattag gtaaagaaaa aaaacgaagt gttttaattg
      841 tattggcagc tttagttcta ataatgggtg gatttactta tttttttaac agtaaatttc
      901 tgtacaatgg gaaaatcgct aaaaatgtat atatagaagg agtaaatgta tctgatatga
      961 ctaaagcaga ggcattaaaa gctataacag ataagtatac tccagaagac ttaaatctta
     1021 cttatgatgg gaaaaaatat acaatatcac caaaagatat tgatttaaaa tatgatacag
     1081 aagatgttgt aaaagatgct tatgaaagta caaaaaaagg gtcctatttt caaaacttaa
     1141 aaaaatatat tgatataaga gtgaataagg caaatatgaa gataaaagct gaatatgatg
     1201 aggcaaaact tagttccaaa gtgtcttcaa tagcagatag tataaatgtt aagatgaaaa
     1261 atgcttctat tagtgttgga agtggtggac taagttatac agactctgtt gtaggtagag
     1321 aatttgattt ggcagcaaat aaagagtcta tatataatat gataaaaaat aaagagcata
     1381 aaactttaga acttaaagta aatcttcaaa aaccagatat aacaactgaa caagtaaaaa
     1441 ctgtaaattc tgttataggt caatattcaa ctacttattc acaagctgta gagggtaggt
     1501 catataatgt aggattatca gcaagaaaaa ctagtgatgt attattaatg ccaggtgaag
     1561 aattttcata caataaactt acaggaccta gtaataaagc aaatggttac aaagatgcac
     1621 ctgttatagt ttatggtaag ctagaacaat cagcaggagg aggtgtttgt caaacatctt
     1681 caacagttta taatgcggct cttctttcgg gtatggagat aactcaagtt acaaatcatt
     1741 catctgcatc tacttatgtg cctaaaggaa gagatgctac tgtgagcgat ggaggattaa
     1801 acttaaagtt taaaaatcca tataagcatc ctgtatatat taaaaattat gcaggtggag
     1861 gaagtgtatc atcagtaatc tatggtaatt ctggtgacaa accaaatata tctatagagg
     1921 taaaacaaac tggtcagaat aaatattcta catatcgaat atttaaagat tcaagtggaa
     1981 aggtcataaa aaaagaacat atatcaaata gttcttataa agaattaaaa aaatagataa
     2041 ttttttagag aaatatttgt aatataaaag ggagtgtctc aaaatgaact ttttagttta
     2101 tgagacactc tttttatgtg aaatcaaata tattttcatc atttcaacaa tacaaaagaa
     2161 gcgtatctct attttgagac agcctttttt gtaaaaatta acactattag ttcttgtttt
     2221 aatattacaa tcattaatat atagaaaata ttcctatata ttggtataat tgtaaataga
     2281 tttagttata taaaatgtat gaggaggtga catggttgaa ttttataggc aatagatatg
     2341 aggtagtaaa ttctgatgac gttttagaaa ttaacaaaat ctataaagct agagatgttt
     2401 tttacaggag aaatgtgtta ataaaagtta taaagcacaa taattatatt tgtgaagatt
     2461 ttgtttcaaa tttaattgat gaaagtactg ctttagatga gataaactct ccttatatac
     2521 taaaaataat agatgttggg attcactgta cagaagaaac aacattatat tatgttgtaa
     2581 gtgaagactt taatggtatc ggactagatg aattaattct aggtaattat cttcatttag
     2641 aagcaattgt aaacataatg atacaagttt taaaagcttt agaaatgata cataggaatc
     2701 attcatatca tggtagttta aaatcgagta gtataatagt agatacagaa tacaatataa
     2761 aaatttgtga ttttggaatt acaaaagcaa ataatggggt taatacaaga agctatggaa
     2821 acaggagata tttatgccca catcaactat gtataaatta tactgataaa gaaagtgatt
     2881 tttttgcaac aggtcttatt ctatttgaat ctatatttaa aaaattgcct tttggagaat
     2941 caaattcaga agaaaaaatg ctacaatcta ttgataaggg attagattgg aatagtataa
     3001 aagcaattaa tggaaataca gaacttataa atgttataaa aaggcttctt ggaagaaata
     3061 ataagtatac tagagccaat gatattatta ttgatttaag taaagtaatg tatgaaaaag
     3121 catatataga agttgaagat gagaaagaag aagaacagaa acaagacaaa gttatacaag
     3181 ttaaaaaaga atataaaagt aaaatgagaa aaaaattaca taaaaaactt gttgttgccg
     3241 gtctagctat agtcatgatt tcaatgatta ttattagttc cataatataa aaattgtata
     3301 ttaagtattt taaaatcaat gtctataaat gatatacata gaaattttag tgtagtctta
     3361 aattatgaaa ggaagtggtt attatgttaa caaaaaataa tgaggtatta aacgtctagg
     3421 agtattttaa caaatagcca cctgctttta cagtttagaa taaaaaagct gtggctaaag
     3481 gtcacagctt tttgctactt tttaaaggca ggagaatatt aaaaaggagc aaattatgag
     3541 caatataaaa ataatagcaa gtaataaatg ttggattgaa gatatagcaa ggaaacaact
     3601 agaagatatt tcaaagttaa atggaatttt aagagtagta ggattacctg acttacatac
     3661 aggtaaaata cctgtaggtt tagcagttga gactaaaaat attatttatc cacatataat
     3721 tggaaatgat ataggatgcg gaatgacttt atttaaaact ggggttctaa aaaagaaatt
     3781 taaaaaagac aaatggatta agtcacttag taagattaaa gatttgtccg atatagaaat
     3841 aaaaaacaca tataaagaag aatgtccaat tttaaattta ggaactatag ggagtggaaa
     3901 tcattttgta gaaattcaat gtataagtga aatttataat aaagaacaat ttgaacaatt
     3961 aaaattttca tctgatgata ttatgatgtt agttcactgt gggtcaagaa attatggaga
     4021 agatatttta aaaaaatttt atgataaaga tggacttgaa gttgaaagtg agaaagcaat
     4081 agattatatt aaaaaacatg ataatgcttt aatgtgggca gagcgtaatc gtgaagctat
     4141 cagtaaaaaa attatgcagt caataggtac atctgaagat gttgaaacaa tattctctat
     4201 aaatcataac tttatagaga aaagggaaga taaatttata catagaaaag gtgctgtttc
     4261 aagtgaaaga ggagctgtga ttataccagg ttcaagaggc agtctatcat atatagttat
     4321 gcctactgaa aatacagaga tatctttata ttctctatca catggagcag gtagaaaatg
     4381 gtctcgttca gtttgtaaat cgcgccttaa aagtaagtat agtaaagata ctataaaaca
     4441 aactaaattt aaaagtcaga ttatatgtaa tgatgtaaat ttattatttc aagaagctcc
     4501 agaggcatat aagaatatag agcagattat tgaaagtttg atagaatatg aacttattca
     4561 agtagtggca actatgaagc cattgattac ttataaagga taatttttaa agttaaaaaa
     4621 gattaaaatt atattataaa tagctaattt tatggaacat ttaaaagtgt tttattatta
     4681 ttagctattt ttttatttta tagagacgat aatttataga gctaaattct ttgatatata
     4741 atgattacaa agttagattg tattggaaac tacacataca tgaattgatg acaatataag
     4801 taattccaaa atataaatga aagtataagc ttattttcac tataaaaatt ttcactgtac
     4861 aaaaacatta ataacattat ttttattgac ataaatatat aatttatgga ataatattag
     4921 aagcactggt ataagtgttt aaataagaat agaaaaggag aagtgtttta tgaaaaaaaa
     4981 tactaacatt aatgagtccg gagttaaact tcttaataaa taaggagttt gatatataat
     5041 aactccttat ttattaagtt gttcgcaaaa taactattat caaagtaaat gatagggtga
     5101 tttaaaagac gatgacttat aaataaagga gaaaaaataa tgaatagtaa tacatttgaa
     5161 agattgcaac taaatgaagt taaagactta ataaaaatac attgtgtaag ttcgctagga
     5221 aaaaatttaa tagataaatt aactcctagt gggaatattg gagtagtaag aaggaaatta
     5281 gcagaaaata aagaagctag aaagataatt gaaaatagta atcacatacc acttgaagga
     5341 ttgtttaatg caggttctac aatagataaa attgaaaaag gaatgataat agaacctgtt
     5401 gaacttgtaa atattgaaga ttttttaaga ggttgtagga agatgaaagc ttttatgtta
     5461 gaaaaagaat tttattcacc tacattaagt tcttatgctt taaatataac agaatgtaaa
     5521 agtatagaag atgagataaa ttactgtata aaatctaata aagttgattc taatgctagt
     5581 aaagaattaa aaaagataag acgaaatata gaaattacag aaggaaagat aaaagataga
     5641 ttaaataaat ttataacatc aactgtaaat aaaaagtata tacaagagtt tataataagt
     5701 aaaagaaatg atagatatgt aataccaatt aaatcttctt ataaaaatga agttaatggt
     5761 actatattag atacatcatc aaaaggaaat actgtattta ttgaacctat tagtgtttca
     5821 aatttaagta cagagttaac tatgctaaaa gctgatgaaa ctattgaaga atacaaaata
     5881 ttatcttatt taacagaact tatatttgag aagataagtc agataaaatt aaatatagag
     5941 atactttcag aatacgatat ggtatttgca aaagctaagt atagtcaaaa aataaaagga
     6001 attactccaa aaataaataa taatggctat ataaaaataa ttaaaggtaa acatccactt
     6061 ctaacaggag atgcagtacc tttagatttt gaaataggca aaaattatag aagtcttata
     6121 ataacaggtc ctaatgcagg aggaaaaact gtaaccctta agactgttgg tttactaact
     6181 ttaatggttc aatgtggatt tgatatttct gcaaaagaag gtagtgaatt tagtgtattt
     6241 gaaaaagttt ttgtagatat aggggataat caaagtattg aaaatgcctt aagtactttt
     6301 tcatcacata taaaaaatat tgcagagatt atgagtttat caaataattc tactcttgtt
     6361 ctgtttgatg aaataggaag tggaactgaa cctaatgaag gagctggttt agcaatatca
     6421 ttattagaag agttttacaa aatgggatgt ataactatag catcaactca ttatggagag
     6481 ataaaaaagt ttgctacatt acatccagaa tttgaaaatg ctggtatgat gtttgataaa
     6541 gaaacgttag aaccattgta taagttaact attggaaaat ctgaagatag taatgctctt
     6601 tttatttcta aaaaaatggg aataaaaaat agagttttag atagagctaa agaatatgta
     6661 attgatagaa actacaattt agatttagtt aaaagaagta agctacaatc taatattgaa
     6721 gataaaaata gttctgaagt cattttaagc tatacagatt ataatgtagg ggataaggta
     6781 aaattactag atgaagatga ttttggaatt gtatataagc ctatggacaa gtttaataat
     6841 gtagaagtat tgtttaatga taattttatt tcagtaaata taaaaagatt acaacttagc
     6901 ataaaagcat cagaattgta tccagaaggg tatgatttag atgtattatt tactagtttt
     6961 aaagaaagaa aattagaaaa agatattcaa agaggttcaa aaaaa
//
LOCUS       DAEPLF010000175         6954 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEPLF010000175 DAEPLF010000000
VERSION     DAEPLF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.175"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            280..615
                     /locus_tag="KR314_002883"
     CDS             280..615
                     /locus_tag="KR314_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329252.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            752..2029
                     /locus_tag="KR314_002884"
     CDS             752..2029
                     /locus_tag="KR314_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBH0329253.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNNSQKDSNNDNNDNG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            2150..3538
                     /locus_tag="KR314_002885"
     CDS             2150..3538
                     /locus_tag="KR314_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0329254.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSESAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3909..4622
                     /locus_tag="KR314_002886"
     CDS             3909..4622
                     /locus_tag="KR314_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBH0329255.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLFLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            4740..5609
                     /locus_tag="KR314_002887"
     CDS             4740..5609
                     /locus_tag="KR314_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH0329256.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSITSNIITV
                     IPEKSRVEVLQVDGDWSKIVYDDKIGYVFNYFLSTDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5764..6909)
                     /locus_tag="KR314_002888"
     CDS             complement(5764..6909)
                     /locus_tag="KR314_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH0329257.1"
                     /translation="MSTYYVPPINLLGKGCLNEAKDSIKSLGTKKAFVVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVDKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIVAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAVEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKEKIIELFMKIS"
BASE COUNT         2746 a          755 c         1169 g         2284 t
ORIGIN      
        1 tattagagtg attttatata taatttaaaa taataaaatg ttaataaaat gagttaaaga
       61 ttgtatatta atagggttga tgtagttaca aaaaagttac caattgttat gtatttacaa
      121 aactgatata ttaattacat aaaatctaaa atagtttgta ataggagtat atataataaa
      181 aattttaatt aaataaagct aggtatattt ataatattaa taaatatatt gctataaaag
      241 tttgtttaat aaattaaata aagaaaagag gagaataaaa tgtacaacat agatgatatg
      301 atatataatt gtccagtaga agctttatca aatattttag gtaaaaaatg ggtggctata
      361 attatatgga aaatacaaga agataagaaa cgatttggag agctacaaag agctatacca
      421 gactgtagta agaaaatgtt agttcaacaa ctagattttt taattgaaca ggggattata
      481 gtcaaccaaa aaaagttggt taataatgtt gtagagtcaa catatttttt aagtgaatca
      541 ggattacgat tgcttcctgt catggaaaaa atgattatgt ggagcaataa aaatttaaat
      601 tgtgataaat actaaaattt actctgtaag taatgaaaat ttaattaagc tatgagtaga
      661 aaaatgtatt aaaattacat aattttttag ttagatttta atttagaaat gggattgtaa
      721 tattggaaat aaaataagga ggaattttaa tttgcaaaat gtaaatttat ttttagtatt
      781 tatagaaggt atagtatcat tcttttcacc atgtatactg ccaattttgc ctatttattt
      841 gagtatattg tcaaatagta gtgtagaaaa tctaaaagaa ggaaagacga gttttatagg
      901 aagttcttta tttaaaaata ctatattttt tgctcttgga atctcaacaa cattctttat
      961 attgggttca tcagtaaaag tattgagtat gttttttaat gaaaataaag atttaataat
     1021 gtttattggt gggattataa ttattatcat gggattattt tacatgggaa taattaaatc
     1081 ttcaatattg aatagagaaa aaagatttaa tgttaagttt aaagaaatga aagcaataac
     1141 agcttttata ttaggtttta cgtttagttt tggatggaca ccttgtattg gtccaatatt
     1201 ggcatctgta ttagtcatgg tttcaagttc aagtaatcat cttagtgcca atcttttaat
     1261 agcagtatat actattggtt ttattttgcc atttataatt acagctatgt tttacagtaa
     1321 actatttaaa actatagata agataaaatc taacatggaa attattaaaa aaataggtgg
     1381 gattatactt atagtatcag gaatattaat gatggtaaat ggatttggaa gtataagcaa
     1441 acactttaat acgtcacaaa atagcaaaat tgaaagcaag caagaagaaa ataagcgaga
     1501 aaattctact gataaagaag aaaattctga tggtaataat agtcaaaaag actctaataa
     1561 tgacaataat gataacgggt ctaatgatga agatagaata aagtctattg attttacttt
     1621 aacagaccag tatggtaaaa cacataaatt aagtgattat gaaggcaaag ttgtattctt
     1681 aaatttctgg gctacatggt gtcctccatg taaagaagaa atgccttata tagaacaact
     1741 gtataaagac tataataaaa acaatgatga tgtagtaatt ttaggagtag cttcacctaa
     1801 tttaggtaga gaaggttcaa gggaacatgt agttaacttc ttaaaagacc aaggttatac
     1861 attccctgtt gtacttgatg aagatggagc attagcatat caatatggaa ttaatgcttt
     1921 tccaactaca tttataattg acaaggaagg atatgtaact cagtatattc caggagctat
     1981 ggataaggca actatggcat catttattga aaatcagaga aataaataaa agtatttaag
     2041 tatatgttta tgaatcaatt taaggttaca aaaaagtgcc taattgtacc acctaaataa
     2101 tcttgttata gtaaaagtat aaacaataca agaaagggtt gaattttata tgaaaaaaac
     2161 atttgatgct ataataatag gatttggaaa aggtggaaaa acattagcag gagatttggc
     2221 aaacagggga ttaaaagtag ctttaataga aaagtcaaac aagatgtatg gtgggacatg
     2281 tgtaaatgtg gcatgtatac caacaaaaag cttagaaaat tcagcaaaca gcgtaaaaac
     2341 aaagaatata aattcttggg atgaagtaca agctgaatat gagaaagcta tagataaaaa
     2401 agaaacttta attacaaaac tcagagaagc taactataat aaattaaatt caaatgagaa
     2461 tgtaactata ttcacaggaa tgggaacttt cattgatgaa aaaacagttc aagttaaaac
     2521 tgaaaatgaa atttatgagt tagtagcaga taatatattt attaatactg gttcaagacc
     2581 atttatacct aatattaagg gaattgaaaa taagaatata gtatatgata gtgagtctct
     2641 tatgaatcta agagttttac ctaaaaaaat gacaattata ggagctggat ttataggttt
     2701 agagtttgct ggtatttata gttcatttgg agcagaagtt acaatattaa actctaataa
     2761 tggcatttta ccaaatgaag atgttgagga ttcagaagaa ataataaaat tacttgcaaa
     2821 gagaaatgta aaaattgtta ataatgcaaa tataaaagag attaaagaag tttctgaatc
     2881 agcaatagta gaatatgaag tagatggtaa atctaaagaa ttaacaagta atatgatact
     2941 tgtagctaca ggaagaaaag caaacactga agggctaggg ctagaaaatg caggtataga
     3001 attaaatgaa agaggattta ttaaagtctc agaaactctt aagactaata aagagcatat
     3061 atgggctata ggagatataa atggaggtcc acaatttact tatatatcat tggatgatta
     3121 tcgcatagtt attaatcagt tatttggaga taagactaga acaactaatg atagaaagaa
     3181 tatcccaaat tctatattta taagtccagc attttcaaga gttggtctta atgttaaaca
     3241 agctaaagaa aagggatatg aagtattagt ggcaaagatg ccagtagaag caattccaag
     3301 agctaaacag attggcaagg cagatgggtt tataaaaata gtgatagata aaaaatccaa
     3361 taagatactt ggagctagta tgatatgtga aaattcaagt gagattatcc atcttattca
     3421 attagctgta gatttagaag tagagtatac atatttaaga gaccgtgttt atgcacatcc
     3481 aactatgaca gaagctctta atgatattct atctcctaat atgataaaag aagtataaca
     3541 taagtttaat atataaacct aaaattatca ttataagtag attttagaaa gaggctatct
     3601 caaaaaatag agataagtct cttttttact ttttaaatga taaaaatata tttgatttta
     3661 taaaagaaga ttatcttatg aactaaaaag tttattttaa aacactacct atttatgtta
     3721 taaacttata aatataatat agaatctatt attctgtatt attaagtgag gttaagcgtt
     3781 ataattaatt ataaatattg aataatctaa atggataaga taatggtatt aaaaattatc
     3841 ttaaaaggtg aaataatctg tatttaatgt agtacagtta tatctttaaa gcaaggtggt
     3901 gaaatgatat aaataagata tctaaaagag ggtttgatga tagtgataaa agatggtttt
     3961 caagtaaaga attaacaaga cttataaagg ctaaagaaga aatagaatgg ttattaaata
     4021 gggattacaa aatagaccca gtcgttactt ttgttggtga tagatatcag ttttctataa
     4081 gacaaagaga tgcattaaaa agagcagtct gtactgatga aaaaaatatt ataagacaat
     4141 caaaaaggct ttttctagat aaaatcaaag aagataccgt aaatatagat ggctttaatt
     4201 tgataatatc catagaggta gcattatctg gtggtactct agtaatagga agtgatggaa
     4261 atataagaga tttagcaggt cttaggggaa cctataagat tatagataaa acagaagaag
     4321 ctttgaatct gataggtaaa ttttttaata aatacaatgc acagaatctt aaattttatt
     4381 tagatgctcc agtttctaat tcaggaaatt taaagtatag gatattagag catgccaaaa
     4441 cttggggaat tgaaactgaa gtagaattag taaaaaatgc agatgttgta ttggaaaaat
     4501 tagatagagt agttagtagt gatgcagtca tagttgacaa gtgtataagt tattttaatg
     4561 tggctagagg tataattgaa gagtatatta aagaatgtaa tatcataaat ttaaataaat
     4621 agataaatag aggtgagaat atataagtat atgtagcaat attattagta atacaagtaa
     4681 agtttgaaaa taggagaacg aatacatatc gaatttcata aaatgttgta aaaagacata
     4741 tgagaggatg gatatgtgtg aaattaacta aactaaatat taaaaaatat agagcaccta
     4801 tatataaata tgctttagca aatgtaaatt tgaggtctgc taaatctact aattcaagta
     4861 tcataacagt aatacctcag ggagctaaaa tggaagtatt ggatgaagaa gatgactgga
     4921 ttaaggtgat gtacaattct caagaaggat atgtgtataa ggatttggta tcagtaagcg
     4981 aatatgcttg gagtaactta aacttaagag aagataaatc tataacttcc aatataatta
     5041 cagtaattcc tgaaaaatct agggttgaag tccttcaagt agatggagat tggagtaaga
     5101 ttgtatatga tgataaaata ggatatgttt ttaattattt tttatcaact gatgggaata
     5161 aaccaaatga attagattat aagtattttt atactgacat gattaaattt gttaacgaaa
     5221 ataatattaa aagtactagc gattatctaa ttgtaacaga ccttagaaat aaatatactt
     5281 atatttttaa gaaagataat ggaggctggg ggcaacttta caagtggcaa tgtactatag
     5341 gcaagcctga gactccaact ataacaggta tattttatat aagtggaaga aaaccttctt
     5401 ttggaacgga tgagtattca gttaagtatg ctactagaat taaaggtgga tattactatc
     5461 attctgtgct atatgattca acaggaagtt atataataga tggcagactt ggtgaagctc
     5521 ttagtcatgg atgtataaga ttaagtacgg aaaatgcaaa gtggatatat gataatatac
     5581 cagatacaac aacagttata attcattaag aaaaattttg agtttttatt gggataatga
     5641 ttctcaaatt aataggatta tatattaact tcttcataaa gagtactatt taaaaagtgt
     5701 aataagagag tgctattaac aaaacaaaga tattttgagg cactctctta taaatataca
     5761 taattaagat attttcataa acaattctat aatcttctcc ttatcaaaga aaacaggatt
     5821 agcaccagca caagcatctt tcatagagtt ttctgcaagc aattcaaaat taggattatc
     5881 aacacctaca tcttttaaag attttgggat tccaacctct tcagaaagag cttttatctt
     5941 atctattaca aagtcaacac actctttatc tgatttatta tttacatcca ttccaataac
     6001 ttcagctatt ggtcggaatt tggcaggggc tgatttagca ttttcctcct caactattgg
     6061 aagtagcatg gcattacata ctccatgagg taaatcatat aaacctccta attgatgagc
     6121 catagcatgt acatttccaa gacctgcatt actaaaggca attccattta aaaaacaagc
     6181 ataacacatt tgttctctag cttctatgtc attaccattt ttaacagctc ttggaaggta
     6241 tttaaatatc tgctttatag catataaggc agttgaatct gtaacatcat aagcaccatt
     6301 agctactaca gcttctacag catgagttag tgcatccata ccagttgctg ctgttaaatt
     6361 agatggtttg ctaatcatta attctgggtc atttactgtc attgtagcta gtgagttggt
     6421 atcaaccatt atcatcttta catgtctgtc ttcatcagtt ataacatagt ttattgtaac
     6481 ttcagcagat gtaccagcag tagtagttat agctacgata ggaagacatt ttttagaagt
     6541 tttgttgata ccttcatagt ctctaatatc tcctccatta gtagctaata tagagatagc
     6601 tttaccacag tcttgtggag aacctcctcc tatagttata acgaagtcac aattttctga
     6661 ttttaatatt ttaagtcctt tatccacatt agttacggtt ggattttgtt taacttcatc
     6721 ataaacaaca aagtctacat ctatctgaga taaaatatct gttacttttt ttacagtacc
     6781 atttgaaact agaaatttat cactaacaac aaatgctttt ttagttccta atgatttaat
     6841 tgaatcttta gcttcattaa gacatccttt cccaagtaaa tttattggtg gtacataata
     6901 agtgctcata actaaaatct ccttttattt tcattgattt ttatgtaaaa atat
//
LOCUS       DAEPLF010000176         6949 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEPLF010000176 DAEPLF010000000
VERSION     DAEPLF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.176"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            166..1524
                     /locus_tag="KR314_002889"
     CDS             166..1524
                     /locus_tag="KR314_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBH0329258.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     ASGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            1630..2424
                     /locus_tag="KR314_002890"
     CDS             1630..2424
                     /locus_tag="KR314_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBH0329259.1"
                     /translation="MVTIKEYSIPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            2417..4723
                     /locus_tag="KR314_002891"
     CDS             2417..4723
                     /locus_tag="KR314_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0329260.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVAESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRIIRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            4862..6139
                     /locus_tag="KR314_002892"
     CDS             4862..6139
                     /locus_tag="KR314_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0329261.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIESLKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            6322..>6949
                     /gene="yqeC"
                     /locus_tag="KR314_002893"
     CDS             6322..>6949
                     /gene="yqeC"
                     /locus_tag="KR314_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBH0329262.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKN"
BASE COUNT         2709 a          760 c         1313 g         2167 t
ORIGIN      
        1 acgtgtattt gttaataatt aaatttattg catctgtttt ttaagactaa aaacttaaat
       61 atatgttgga gtatagttga tatttatgaa catcattaat ttaatatatt agatttttaa
      121 gtatttatat aattcaataa gttttatgta acgggagttt ttattatgaa agcaaaaagt
      181 acatcaaaat ttgaattaga cggaatacca ccactaaagg aagcaatacc tttagggtta
      241 caacacttaa tggcaatggt aataggtagt tgtttaccag cgatattgat tgcaaatgca
      301 tctggactta atcataagat gtcaacatta ttgattcaag ccgcattagt ttttgcagga
      361 ttatcaactt tattacaatt atatccaata cctttgttta aaggttttaa attaggagga
      421 ggaataccag ttataatggg agctacagct atgtttatag gagctggggt atctgttgca
      481 agtaagtatg gactaccagt attatttggc tctcaaataa tagctggtat agtaataatt
      541 ataattggtt ttggtcttaa aaaaatacga tttatattta ctccagctgt atcaggaaca
      601 atagttgcat gtatgggtct tggacttttt ccaatagcta taaaaaactt agctgggggt
      661 atgggcaatg agacttttgg gaatccaatt aattttatag ttggaatatc tgtatctctt
      721 atgatattag gattaaataa gtatggaaaa ggattattta aagatgcatc aatattagtt
      781 agcataattt ttggatacat tttatcatta attttgggta tagtaaattt ttcttcaatt
      841 caggagttta ctttggttgc gcttccaaaa ccacttgctt ttgggctaga tataagactt
      901 gaagttgttg taatgttttc aataatatat ttagttgaga tagctgatat aatgggtgct
      961 tgtacacttt ctgcggtagg agggcttaat agacaagtaa cagatgaaga actttcttct
     1021 gctgttttag gaaatggagt actctctaca ataggtgcat tattttctgc aactccaatg
     1081 gggatgtttg gacaaaattc tgcaatcgtt tccaatacaa aggttgtaag taaatttgta
     1141 ctagctattg gaggaattgg attatttttg gcaggaatat cgcctttatt agctaattta
     1201 ataagaacaa ttcctccctg tgtggttgga ggagcaacac tcgttatatt ttcaactctt
     1261 acaacatctg gtttaagact tgttagtatg gatggattta atcaagaaaa tagcatgata
     1321 ttaggtcttt ctatggcttc tggtattgga ttcatggtag ctcctcaagt tcttgaaaaa
     1381 tttccaaaat ttatagagac attacttgca gattcaagtg tagtttcagg agcaatggta
     1441 gccataataa ttcaagcttt atatatggta aaatttcctg gaaataaaag tgataaggtt
     1501 gaggataaaa ataaaagttt ataatgtaaa ggtttaatat atgtgaatta aatatactac
     1561 aattataaat ataaataaaa gatttattaa aaacttttgt gttttaataa gttaatgatg
     1621 ggggataaga tggttactat aaaggaatat tcaattccac agagtttaga agaagcatat
     1681 aaaattttaa tttctaaaaa aaataatgtt attttaggtg gatgtggatt tataaaatta
     1741 agtaataaaa atataggaac agcgatagat ttaaaagata taaatctaaa ttatataaaa
     1801 gaggatgaaa aaaatatttt gataggtgca gatacttcac ttagaagttt agaaattgat
     1861 aaaactataa aaaattactg tagtggaata ctttcaagcg ctgtttctaa tatagttggt
     1921 gttcaattta gacaaggggc tagaattggt gcaagtgtat tttcaaagta tggcttttct
     1981 gacttaatac cagcgctttt ggttgttgga gcgaaggtga gattatacaa taaaggaata
     2041 ctagagttaa gtgagttttt ggatagtgaa ttaagtaggg atatattggt cgaagtaata
     2101 ctaccaaaaa aagatgcgat agctgttttt gactcaataa gaaaatgcac aggagatttt
     2161 tctgtattaa attcagcaat gctaaaagaa aatgatactt acaaaatagc aattggggca
     2221 agacctcaaa aagctagatt agcacttgaa gcgagtaata tattaaataa agaaaaagat
     2281 atagataagg catctattgt tgctagtaag gaacttactt atggaagtaa tatgagagct
     2341 agtaaagagt acagaaagga tatggcaagt gcattagtta aaaggatgta taatgctatt
     2401 gaggagggaa tgtataatga ttaataaggg gataagaaag atagatagta cagcaattgt
     2461 cacaggaaaa cctctatata ctgaagacct cattatgcat aaagatgttt tgacattaaa
     2521 actacttaga agtccacatg ctcatgctaa gataaagaat atagatacat caaaagccga
     2581 aaaaattcca ggggttgtgg gtatttatac gtataaagat gtaccacagg tcagatattg
     2641 tgtgccaggt caaacagaac cagaagcatc accttatgat agagttttac ttgaagatac
     2701 agttagattt attggagatg aagtagcttt agtggctgct gaagatgaga aaacagcact
     2761 taaagcctta aaaatgataa aggtagatta tgaggtttta aagcctgtgt ttgatgcaag
     2821 agaagcagaa ggaaatgaag ttgttataca tccagaaaaa gacatagtag agccataccc
     2881 ttttggattg gatgcaaaaa ataatatagt atcaaaagaa ttttttgaat ttggagatgt
     2941 tgaagctaag cttaaagaaa gtgattttgt ggctgaatct tcatacaaaa cccaaagtca
     3001 atcgcattgc atgatggaaa ctcataggtc atattcatat aaagatataa atgatagaat
     3061 tgttgtagtt tcatcattac aatcaccaca caatgtaaag agaataatta gaaaagtatt
     3121 gggtatacca tcttcaaaaa taagagtaat taaacctaga ataggtggtg gatttggagg
     3181 aaagaatata gcagcaacag atatatttac tgcatttgtt actatgaaaa ctggtagacc
     3241 agcaaaattt gtatttacaa gaagtgaaac ttttgtaggt tctaatagta gacatgaaat
     3301 gttttttgat gttaaagttg gtgcagataa agaaggaaat ttaaaagcaa tagagctaaa
     3361 gtcattaaac aatacaggtg cttatggtgg gaatggtgtc tctgtttctt ccgaatctgg
     3421 acacaatacc ttaccaatat ataatgatat tgatgctgtt agatttaatg ctagaacagt
     3481 atatactaat aggttaccag caggagcact tagagggtat ggtgctacac agggaatatt
     3541 tgcaatagat agtgctattg atgaattggc acataagatt ggaatggacc cacttgaatt
     3601 tagaattaaa aatataatag ataggtatac caatggaaaa gcagctgaag acgaaatatt
     3661 aagttgtgag ctgagacaat gtatagaaaa gggcaagaaa ttaatcaatt gggatgataa
     3721 atatccatgt aaagatatag gaaacaacaa agtaagagca gtaggtatgg cagtagcaaa
     3781 tcatggttct ggtataccgc gtgtagatat ggcaacagtc actataagaa tggatgaaga
     3841 tggcacatat aaactattat ctggtcttgc tgacttaggt caaggggctg atacaattca
     3901 agctcaaatt gctgcggaag ctttaaatac tactgtagat agaatttcta tttatacagg
     3961 tgattcagat ttatgtccct atgatacagg agcatttgct tcttgtacaa caacggttac
     4021 aggaaatgca acaataaaga cagcaaataa attaaaagat atattacttt cagttgcaga
     4081 gtcaaagcta ggatttaaaa aagaagattt agagttggga aaagacaggg tttttgttaa
     4141 aaacgataat agcaagtttg ttctattaga ggaattgggt agagaatctg ttggaggaat
     4201 aggatttgct agtgatgatg taccagtagt tttggaagca acagaatctt ttggcatgtt
     4261 gaaaaaaata aaaccattta tagcaggatt tgcagagata gaattagata aaaatacagg
     4321 cgaatataag gtcataaatt ttgcatgtgt acctgattgt ggaacagtta taaatccaac
     4381 tttagctaga atacaagtag aaggtggtgt gatgatgggt atagggtttg caatgtatga
     4441 agaccctata tttgataaag atgggagatt attaacagat agtttcctac agtataaagt
     4501 tcctacaaaa aaagaagttg gaaatatatt ggtagatttt gctgagaatt tcgacccagg
     4561 aggtccttat ggtgctaaat cattaggaga aatagttata catacaccag cgccagctat
     4621 agcaaatgca atatttaatg ctacaggagt tcgtttaaga gagcttccta tgacttttga
     4681 aaaagtttat atgggtatga aaaataataa taaacaatta taataaataa taaggtgtat
     4741 aaaagcaagt aaatttaaat tattttgtaa aataaagtaa ttaaatggac attatatatg
     4801 gtgttttgta aaaataccgt atataatgtc tagtagagta tattctaaag gaggtgagtt
     4861 catgtcaaac agaataaaaa atatacacta tggaactcag agacaacaat taaaaaactt
     4921 aactaaaaaa gaatatcaag ctctacgaga gatgtgcttc ttagctaaga acttatataa
     4981 tgttggtaca tataatgtaa gacagcatta tttcaatcaa ggagaacatc ttaactatga
     5041 agctaattat catgtatgca aagaaaatga gaactataag cttctcaata gtaatatagc
     5101 gcaacaaata ttaaaagaag tagacagttc gtttaggtca ttttttgggt taattaaatt
     5161 agccaaagaa gggaaatatg actttagagg gataaaatca cctaaatatt tagataaaga
     5221 aggatttttt agcataataa taggtcaaat aagaatcaaa gaagatggga ttttaaatgt
     5281 acctatgtct ccagcattta aaaaactata tgggaaagta agtataaaag ttccacaaaa
     5341 tatactagag aaaaaaatta aagaaataag aataatacct aaacataaag ctaggttctt
     5401 tgaaattcag tattgttatg aaataccgaa atcagaggaa gtttcaaatc aaaaaaatgc
     5461 actagctata gacttgggtt tagaaaattt atgtacatgt gttaccaatt taggtgatag
     5521 ttttatcata gatggaaaaa ggctaaaatc tgtgaatcag tgggcaaaca aacagaatga
     5581 taaaatagaa tccttaaaat taaaaaagaa tataactgca ataacaagaa aacaatttaa
     5641 agtttggaat aagagaaata atcaagttaa agactatgta aataaaacct gtttttatat
     5701 aattgattat tgtgtaaaaa ataatattgg aaatttaatt gtaggatata atgctaagtt
     5761 agaaaaaagt gataatatgg agagaaaaat atatagaaat tttataagaa tcccatttgg
     5821 agagataaaa ggaaaattag aatatctatg taaaataaaa aatatcaatt ttattaggca
     5881 agaagaaagc tatactagta agtctgattt ttttgctgat gattttctac ctgatatcaa
     5941 tgtggacaat gttaaaaaat atgactttaa aggggaaaga ataacacgtg gacagtacaa
     6001 atccagtgtt ggaatcatta taaatgcaga tgtaaatgcg agtcttaata tactaaaaaa
     6061 atcacaagta gttaatttat ctaatttaca gaataatcct caaatattaa agcaaccaca
     6121 gagagtaaga atatcttaat tatatttaaa agataaactg atagcgtaag cttacagctt
     6181 agcggcgaat tggatccgcc ttgttgttta caacagaatc tatttacttt cgagtttatg
     6241 gaatttcaaa aatcactttt actaaaaagg tctaactata ataattaaga gattagaaaa
     6301 ctggttataa gaggtgaaag tgtggaacta tcaaatataa tagaaaagaa tgaaataata
     6361 actgtagttg gagcaggagg aaaaacttcg tttataaact attttgcaaa tttttataga
     6421 gataaattga aagttcttct tacaacaaca actaaaatat atgtaccaaa cgattatgac
     6481 aatataatta ttacaataga tggaactgtt attccatcta tttgtcatgg aataacagtc
     6541 tgtggcagtt atataaataa tgaaaataaa ttagtaagta ttgattcctc tattttggat
     6601 gaaattgtag accaatttga tttagtttta atagagggag atggctcaaa gagaaaaaaa
     6661 cttaaaggtt ggaatgctaa agaaccagta gtatatcata aaactacaaa aaccataggg
     6721 atattagata taacatcatt tggaatgaat attaatgaag agaatataca tagagtagaa
     6781 atatttaaaa aaatagcaaa tttagataca agtagtatta atagtagttc tacagtaagt
     6841 attgaaaact taaaaaatat agtattaaat ccaaatggat tatttaaaaa ttattctgga
     6901 aaaagagtat tatttattaa taaagttgaa aatgaaaaat ataaaaatt
//
LOCUS       DAEPLF010000177         6947 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEPLF010000177 DAEPLF010000000
VERSION     DAEPLF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.177"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..981
                     /locus_tag="KR314_002894"
     CDS             <1..981
                     /locus_tag="KR314_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0329263.1"
                     /translation="DKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALRDLDNAYK
                     KFFKEKVGFPKFKSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKVRDKQVPKGRI
                     LNATISKEPSGRYYVSLCCTDVDIGVFENTNNQIGLDLGIKEFCISSCGEFIENPKYL
                     KKSLNKLAKLQRELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKEND
                     IICIEDLQVKNMIKNRRLSRLISDVSWSEFIRQLEYKANWHGRKIVKVGKFFASSQIC
                     NKCGYKNEEVKDLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            1129..2193
                     /locus_tag="KR314_002895"
     CDS             1129..2193
                     /locus_tag="KR314_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329264.1"
                     /translation="MFLKLIFKYNTLDFSRAYFDLTDWADNPKDISYYLSNGTFGDLM
                     DSIDENASKMSLSDIAKLEKKIAKKHINLWNEGKIDFSTTRFSSIVPMLQNAGVNFHF
                     IYPDIDLLKNTFNNLIKDIHLHRMKGNQPSVIYITVKDTNYDDSTLDQVYKILNSIKK
                     DKLTDFIIEKNSNSFKIFTKCETIKSLTKNFTACYIKESIENSTNIKVCVGYGIGNDI
                     KQAMSSAISANKESKINPLNYSFLVNENYETIGPLLCDKLLTVSNKVTPHINTIAKSS
                     KLSTLTIQKIISVTEILQRDEVTPKELSSCLNVTVRTANRFLSSLLKSGNATILYEKQ
                     NSTKGRPERVYKLFISENKY"
     gene            complement(2321..3511)
                     /locus_tag="KR314_002896"
     CDS             complement(2321..3511)
                     /locus_tag="KR314_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0329265.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEESTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEIIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(3617..4996)
                     /locus_tag="KR314_002897"
     CDS             complement(3617..4996)
                     /locus_tag="KR314_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0329266.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELSAY
                     GHGCGHNLLCTAHVGAVVGLKREMLENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(5027..6235)
                     /locus_tag="KR314_002898"
     CDS             complement(5027..6235)
                     /locus_tag="KR314_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329267.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNVLPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFRLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
BASE COUNT         2455 a         1176 c          872 g         2444 t
ORIGIN      
        1 gataaagaaa cttttactta taaacagtgt agttctgatt taaccaattt aaaaaaagaa
       61 ttaaagtggc ttaaggaacc tgacaaattt tcactacaaa acgccttaag agatttagat
      121 aatgcatata aaaaattctt taaagaaaaa gtaggatttc ctaaatttaa atcaaagaaa
      181 attaatagat tttcatataa aactaacttt acaaatggaa acattatgta ttgtggtcaa
      241 catataaaac tacctaaact tggtatggtt aaggtaaggg ataaacaagt acctaaaggt
      301 agaatactta atgctactat atctaaagaa ccaagtggta gatattatgt atcattatgc
      361 tgtactgatg tagatattgg agtatttgaa aatactaata atcagatagg tttagattta
      421 ggaattaaag aattttgtat ctcaagttgt ggagaattta tagaaaatcc taagtatctt
      481 aagaagtcat taaacaaact tgctaaatta caaagagaat tatcgagaaa aacaatcggt
      541 agtttaaata gaaataaagc aagattaaaa gttgcgagac ttcaagaaca tatagctaat
      601 caaagaaatg attttctaca aaaattatct actaaattaa taaaagagaa tgatattatt
      661 tgcatagaag atttacaagt aaagaatatg attaaaaatc gtagactatc tcgcttaatt
      721 tctgatgtat catggtcaga gtttattcgt caattagaat acaaggctaa ttggcatggt
      781 agaaaaattg taaaagtagg taaattcttt gcaagttcac aaatatgcaa taaatgtggg
      841 tataaaaacg aggaagtgaa ggacttaaat ataagagaat ggatttgtcc tagttgtaat
      901 gaaactcacg atagagatat aaatgcaagt ataaatatat taaaagaagg attaagacta
      961 ataacaattc aaaataaata acttatgtaa gaaccgtagg aactacgggg atggcctgtg
     1021 aagaattagt aagacatatt ttagtatgca aaattctatg aagcaggaac cctgtgactt
     1081 tagtcatggg aggttcagtt tggtactgac ttacaaagtt actatgatat gtttctaaag
     1141 cttatcttta aatataatac attagatttt tctagagcat actttgactt aacagactgg
     1201 gcagataatc caaaagatat atcatattat ttatcaaatg gcacatttgg agacttaatg
     1261 gatagtattg atgaaaatgc atctaaaatg agtttatctg acatagcaaa acttgaaaaa
     1321 aagattgcaa aaaaacatat taatctatgg aatgaaggga aaatcgattt ttctactact
     1381 cgttttagta gtattgttcc tatgttacaa aatgcaggag taaactttca tttcatatac
     1441 cctgatatag atttattaaa aaatactttt aataacttaa taaaagatat tcatttacat
     1501 agaatgaagg gaaatcaacc aagtgtaata tatataacag ttaaagatac aaattatgat
     1561 gactctactc tagaccaagt ttataaaata ttaaatagta ttaaaaaaga taaattaacc
     1621 gattttataa ttgaaaaaaa tagtaattct tttaaaattt tcactaaatg tgaaacgatt
     1681 aaatcattaa ctaaaaattt tactgcatgt tatataaaag aatctattga aaatagtaca
     1741 aatattaaag tatgtgttgg atatggaatt ggtaatgata taaagcaagc tatgtctagt
     1801 gccataagtg ctaataagga atctaaaata aatcctttaa attacagttt cttagtcaac
     1861 gaaaattatg agacaatagg acctttactg tgtgataagt tacttactgt ttcaaataaa
     1921 gtaactccac atataaatac aattgccaaa agttctaaat tgtctactct tactattcaa
     1981 aagattatat ctgttactga aattctacaa agagatgaag ttactcctaa ggaattatct
     2041 tcatgtttaa atgtaacagt tagaactgct aatagatttc taagtagtct cttaaagtct
     2101 ggaaacgcta ctatattata tgaaaaacaa aactcaacaa aaggtagacc tgaaagggta
     2161 tacaaattat ttatatcaga aaataaatat taaaatccaa aatgcataaa aatattttat
     2221 catctaatat taagtaaaat tatatatgac tttctaaaag gtatatcttt gcatcttttt
     2281 aagatatacc ttttatgata tttaataata ttttatgtta ttatctacta aaattatttt
     2341 ctaaccattt tactgcacaa tgagcaaata aagctgaacc ataaactaat ccctcttcat
     2401 taaaaattgc tttaggatta tgcataccaa aattatatcc ctgactaata cttcccattc
     2461 caatccataa aaatactcct ggaaccctta gtaaaatctc tgaaaaatct tctgaaccca
     2521 ttgcagatgg aaagtctatt acattctcat ttcctataat ttctttaaca taagatacaa
     2581 tctcatctcc tacatccaaa tcatttataa ctgcgggagt ccccatttta aattccactt
     2641 tagcatttcc tccaaatgat ttagctattc ctgtagatat ttcaataatt ctatttttta
     2701 atttttctct aacaaattgg tcaaatgttc ttaaagttcc tgacatagat gctttatttg
     2761 gtatagcatt tggtacgttt cctgcattaa acataccaat agttaaaact acaggctctt
     2821 tctgaggttt ttctcttgaa agtatagtgt ttaatgctat ttgtatatgc gacattatgt
     2881 ttattgggtc cactccaaat tcagggcttg ctccatgagt tgcttttcct tctactgtta
     2941 tttcaaatat atccgcagat gccatagctg gaccacgtga ataagctact tgacctgtgt
     3001 taagaaattt acccaccata ctgtgaaatg ccatagctgc atcgacctga ggattttcta
     3061 atataccatc atcaatcatt gattttgcac ctctggtact ctcttcatct ggttcaaata
     3121 caaactttac tctaccttta agtaagtgct tattatccat aagtatttta gctgctccta
     3181 agagcattgc tgtgtgatta tcgtgtccac aactatgaga tgcatcttta ttcttacttt
     3241 taaaaggtaa atccgtctcc tccataatag gaagtgcatc catatctgcc cttaataata
     3301 aggtctttcc accattttca ctgtctaaaa cagctactat tccattatta gacgtcaatt
     3361 tcggagaata tcccattttt tctaaagtac tggtaacaaa ggttactgtt ttcggtagat
     3421 tgcaaccaat ttcgggattt tcatgtaaat atcttctcca tctaataatt tccccttcta
     3481 ttgactgcgc ttctttaaaa aaatttttca tattccttcc tcctaatcag atagtcagca
     3541 aaatctcact ttcttttagt aatgaaatga tgctgatata aaaattataa ttttatatat
     3601 taaacttcat gtctttttaa ggagttggaa tatcatctgg aataggacat ttatatgttt
     3661 taccattcat agatttatca aactcttctt ttgccttttt agctatctct ggattattta
     3721 atattttggc tccaaacata gccatgattt ttgatgcata tatcatacct ttttctccta
     3781 tagaacttcc tgcacaagat gtaaactgcc agctatgacc tggtgcacct agattcgtac
     3841 accctgtaaa aaagtatgct gtcggaacta tatgcattac atctccaata tcagtagaac
     3901 cataactatt aaagtttgta acatttcctc caccataata tagatgagtt ccttctggca
     3961 aattatattt acttatcata gccttatatt gttgtgggct ttgtctatct aattcagctg
     4021 caaaatccaa ttcctctcga gtccaaggct cttgtttaat ctcattcata cattctgata
     4081 caagatttgc taaaacatga ttatttaaag tattataaca acctcctaaa aactctactt
     4141 caacttcagt ttcagtcatc attgcagctc cttttgctac ctttacaagt ctttcatatg
     4201 tactttctac agcttctcta cttaaagctc ttgtataata ccatactgat gctttatcag
     4261 gaactatgtt gggtgctact cctccatctg taattgtata atgaatcctt acatcagaag
     4321 taacatgttc tctcaaataa ttagctccca cattagtaag ttccacagca tctagtgcac
     4381 ttcttccatt ttgagggtct gagcctgcat gtgcagtctt tcccttaaag tgaaatttta
     4441 cagagttaac agctgttgaa gtaccaattg tagcttcact aattttattt ggatgaaaat
     4501 ttatagctaa gtctaaccct tcaaatgctc cacctctagc cataaatcct ttacctgtta
     4561 attgttcttc tcctggacat gcataaaata ctatagtccc acttaagtta ttttctagca
     4621 tctctctttt aagtcctact actgccccta catgtgctgt acataataaa ttatgcccac
     4681 atccatgacc atatgcactc aattcaaatg cttcttgctt tgttgaaact ttttgattta
     4741 aaccaggtaa tgcatcaaat tctcccataa aacctataac tggtttacct gaaccaaaac
     4801 tagctctaat agccgtggct aaacctccta cacctacttc cacatcaaat ccttcacttc
     4861 ttagaaagtt tgctgtattt tcacatgctt taaattcttt aaatgctttt tctgggtttt
     4921 cccatatttt cttacttaaa tcatcaagtt tgaatctttt tttatctatc tcatttatag
     4981 atatacttcc tatcatttct tattcctcct ataagtatat ttactatcaa ccaaatataa
     5041 ttggacctat tatactagca aatgccactg atgcaatggt tacagtaaca aatcctccta
     5101 taatcatctt tggtaataaa taatctgttg ccctttgttt ttcttcttca gttccttcca
     5161 tagcatcaat accttcgttg gaaattatct gtgttgcagg ataagctagc atacaagtta
     5221 caccaattgc agctgacatt cttggagaat atcccaatag ttttcctaca attgcagtaa
     5281 ataatattat aaatacagaa cctacaacaa gtattcctat agttggaaca atcatcccaa
     5341 acaatgctgt tggagttacc ttagcaagtc ctccaggtaa catcaacata caaccaaaca
     5401 ttataattcc attagattga gatttatcta aaattccttt ttctaaaaaa cctattcgtg
     5461 taaaaatcaa ccctactaac aaacatgcta tattaggatt taaataatag tttgcagggg
     5521 taaatcctgg tattaaagta gccaatccta caaaattacc aatcatagca acaaatgaaa
     5581 gctttgcaag atacactaga gtagatgatt ttttacctaa atcttcttta aaaattctca
     5641 tacttggaag tctaaaatta ctttttgact catctttcaa aaaatccccg ttatctcttt
     5701 taaattttaa ttctttattt atacaatatg tagttatcgg aatgccaaag aacttttgaa
     5761 atgataatac tagaattaca aatgcagcaa gttcaggtct atttgcagct tctgcaacag
     5821 ttgttacgat aataccggca acagtagaac ctgctattgg aggtgctgct attaaagcat
     5881 actctctacc aaataacata ctacccactg ttaaacagcc aacccctatt cctacaatac
     5941 ctactaatgc aattacaacc gttttccatt catgacataa gtcttcaaga tttataagtg
     6001 ttcctaagtt agttattaat aaagctatac caaagttgct cattgcagct aatatccctg
     6061 atgctcctgt aatgtctttt ggtaaaacat ttgtccaaaa tcctagtaaa aatataatac
     6121 atacaaataa caatgttgaa attgcacctt ttgttttctt agataaaaac tcactaaaag
     6181 cataaatcat tacacataca gtaaagcaag taagtggatt ccaaaaattt tccattgttg
     6241 tttcctccta aatataataa ttttttatca ataaaacaat aattataaaa tgtatattat
     6301 gcagttattc ttaactacat atggtatagc ttgatatttt ttgatgatat tatatacgtc
     6361 ttaaattatt cctaaaatat ctaaataaat ataactagag ttaactctct aaatattaac
     6421 tataattatc aacttactag atttcttttt gatttttact ttctacataa ttttacaagt
     6481 tagaattgta cttcaactac tttctatctt agtatacatg ttggaagact tatatttatt
     6541 taaatattag atatagaata catttttaca aacgtcttaa attattctta aaatatctaa
     6601 aacgaaataa taatataacc attaaaccaa tattatcaga atatactcca ctttaagtct
     6661 tctttatata aaaattttat ctactagtgc atcccctcct ttgtaaatta gactatttct
     6721 aagcgaattt agatatctta ttcctatgaa ctattactag aaacaaaaat tatttattat
     6781 attaaatcaa tgataagcat actttaaaat tctttctata caagtatgat ttatcatata
     6841 atgacatcta taccttcatc tttatagtag tcctaaatta ttcttaaatt gacaataaaa
     6901 ataatttcac tatttgtaaa aatacttgtt tttatattgt caaacta
//
LOCUS       DAEPLF010000178         6946 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEPLF010000178 DAEPLF010000000
VERSION     DAEPLF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.178"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            223..837
                     /locus_tag="KR314_002899"
     CDS             223..837
                     /locus_tag="KR314_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBH0329268.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            855..1217
                     /locus_tag="KR314_002900"
     CDS             855..1217
                     /locus_tag="KR314_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329269.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYKGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1323..1820
                     /locus_tag="KR314_002901"
     CDS             1323..1820
                     /locus_tag="KR314_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBH0329270.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
     gene            2244..2642
                     /locus_tag="KR314_002902"
     CDS             2244..2642
                     /locus_tag="KR314_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0329271.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSEKYKLKARNVK
                     INLNIKGYKFLPYVKNLKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            2630..3568
                     /locus_tag="KR314_002903"
     CDS             2630..3568
                     /locus_tag="KR314_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBH0329272.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIRH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKTIILGVCGKLILVPSIFLPISIFLGFRNVELTSLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(3952..4176)
                     /gene="secG"
                     /locus_tag="KR314_002904"
     CDS             complement(3952..4176)
                     /gene="secG"
                     /locus_tag="KR314_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBH0329273.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(4294..4800)
                     /locus_tag="KR314_002905"
     CDS             complement(4294..4800)
                     /locus_tag="KR314_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0329274.1"
                     /translation="MNLEIIRATSENAIEMGFIHSCSWRKAYKGIIPDKIIDEFTSEK
                     RAEVFQSVIPKAEEEYYLFKVNGIPAGFASLNRSQEDNAPKCVGEIYSIYFHPDFWGT
                     SVTKKGLQFCIDRLSNLGYSYITIWVLKDNTRAINFYKKNGFTCDDFEKEIHIGKKLL
                     EVRYSKKI"
     gene            4885..5313
                     /locus_tag="KR314_002906"
     CDS             4885..5313
                     /locus_tag="KR314_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0329275.1"
                     /translation="MNPVKELNNLWHIVSMQMKTEMCSGKYPTIHGISLVELSILEIV
                     EQYPNSMMKEISEILELPKSTLTSAVKRLELKKFIKKNPCIKDKRRYNLVLTELGLKA
                     QQEHRDIEIIVFRNLLHQLDKDEIETFIRIFSKAVNKSKK"
     gene            complement(5433..6725)
                     /gene="eno"
                     /locus_tag="KR314_002907"
     CDS             complement(5433..6725)
                     /gene="eno"
                     /locus_tag="KR314_002907"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBH0329276.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTERLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         2478 a          997 c          913 g         2558 t
ORIGIN      
        1 taactcaatt aattttttaa atacaacttt ataatataaa acttataata aaagtaaatt
       61 tatatgttaa atgaggttga ttttttatta ttttttattt atgctagtac taaagacttg
      121 caaacgtttt ttgacaagtc tttattttgt ctaaattaaa caaacttttt tacaaataaa
      181 tacataaaat ttaaactaat ctatttattg gaggagataa aattggaaga agtaattgca
      241 aaaattatta ctatagaaaa tggattcaaa gaaattgaaa acattgctaa aaaaattgta
      301 aaaaataatg actcaaaaaa gtgttattat ctatcaatct acttatatag gtcagaatat
      361 tatcaagtaa gagaattggc tgtgtttata cttggtttta tatcagtaac tatttctgaa
      421 gctttgttat ttttaaaaga caatgtgagt aaagatgaaa actggagagt acaagaaata
      481 ctagctaaat catttgatta ttattgcagt gaagtaggat atgaaaaggc acttcctgtt
      541 ataaaagaat ggcttaattc tgatagtcca aacataaaaa gggctgtgac agaaggtctt
      601 agaatttgga ctagtagaga ctattttaaa tctaatcctg acgttgctat atcactactt
      661 tctagtttaa aagaagatga tagcgaatac ttgagaaagt ctgtaggtaa tgctcttaga
      721 gatataagta aaaaatatcc agatttagta aaaaaagaat tagattcatg ggatatatca
      781 aataaaaaag ttgagtttgt atataagctt gcttcaaaat gtatcttggc aaagtaaaat
      841 ggaggtgaac ttatttgaaa cttcgtgatg agtatacttg cccattagaa ctaacacatg
      901 atatgataaa aggcaaatgg aaatctatta tattatggag attacgcata ggaccaacct
      961 ccttatcaaa acttgaaaag gaaatcaatg gtattaatca aaagatgtta cttgaacaac
     1021 ttaaagaatt aattgaattt ggtcttgtag ataaaaagac atataaaggt tacccactta
     1081 aagttgaata ctcacttaca cctattcgag ggaaacaaat attggatgct ttggctataa
     1141 tgcaagatat aggaattagc tttatgttgg ataatggtat ggaagatatt cttaaggaaa
     1201 aaggtgtttt aaaataaata gatacccaca aaaaagtaag taatttacca ttattttcaa
     1261 ctggtatata attaaattat agatatattc tattaattta ttataatagg aggtaaatta
     1321 ttatgaaagt aactagtaca ggtatagtaa atggtgttat agaagataaa tacggtaaaa
     1381 gaggaactca gttcaatgaa ggtggtatgc ctacatattc actacctcta aagtttgaag
     1441 atgctccaaa gaatacagta tcctttgctg tatttttaga agataaagac tctgttcctg
     1501 tatgtgggtt tgcatggata cattggttag ctgtaaatat aacaaaagat gagttaaatg
     1561 aaaatgaaag tgtaacagct actgattttg tacaagggac tactagttgg catggcaaac
     1621 taggaggtct tgacagatta gaggccagtg tatatggtgg aatggctcct ccagacagac
     1681 ctcatgtcta tgaaatacat gtatttgcac ttgatactat ccttgacctt gaaaaaggat
     1741 tctatatgaa tgaatttttc aaagctattg aagggcatgt tttagataca tttacactaa
     1801 aaggaagcta ttctaattaa ttaggtttac tatagctaat cattaattaa atgctacatt
     1861 taattgatga ttagcttaat tgttattaga atattttctg cactttatct taatatatat
     1921 tttagttgac tggtatttta atttctatta gattattatc aatttatata aattgatttt
     1981 agcttaaaac ttacttatct atttcagttt ctattagaat tattattaga ctcattaaaa
     2041 taaaagcatt aatataacta ataatatatg ttaaattcat taactctaca taaagtttta
     2101 taatctaaat gaaatacaat aatctgcata aaattcaata gcttatataa aatttagtaa
     2161 tttatataaa tattatcaat ctatataaaa tttaataact tatataaatt taactaattt
     2221 atataaaatc ttaaggagtt gccatgataa aaaaaataga gcatgtggtc attactaata
     2281 ataatataga aaattatatc aatttttata aaaaatttgg tttttcatct aaagaaatga
     2341 gcgaaaagta taaacttaag gctagaaatg ttaagataaa tcttaacata aaaggttata
     2401 aatttttacc ttatgttaaa aatttaaaag caagtagaga taacttatgt tttaaaataa
     2461 acacaaatat tgatgagcca aaaaaatact tagaacaaaa tgagattcaa attaagcgag
     2521 gaattgtaga atgtacaggt gtctttggta atatgaaatc tatttatttg aaaaaaacta
     2581 atggaaattt aattgaatta tcaaatcata gaatcacgga gagtaaacct tggaaaattt
     2641 aatcctatca ataaatgttg ttcttccttt gtttctcaca atgtcacttg gatatgttct
     2701 aaaaaaatta ggtctatttg atactcatac tttaaataaa atgaataatg tctgcttcaa
     2761 gtctttcttg ccattattat tattttttaa tatatacaat acagatttag atggttctct
     2821 taattttaag cttatggctg tagctgttat aattattttt tcaatcttct taatcttagt
     2881 ctttattatt cctaaaattg aaaaagataa taagacaagg ggtgcattaa tacaaggtgt
     2941 atttcgtagc aatttcgtta tatttggtat tcctgtgaca atttcactat ttggcgataa
     3001 gagtattgga gtcacatctt tattaattgc agttattgta cctatgttta atgtattatc
     3061 tgttgtaatt cttgaaattt ttagaggtgg caaagtaaat ataagacata tgattaaaag
     3121 cgtcttaaca aatccactca taatcgcctc ttttatagga ttgacaatgc taattttaaa
     3181 tattaatata cctacatttt tacacaaaac tattggtgat atttctaaaa tagctactcc
     3241 tttatcactt ataattctag gcggatcttt tagtttttct gctataaagg ggcatataaa
     3301 aaccattata ttgggcgtat gtggaaaact tatacttgta ccttctatat ttttaccaat
     3361 tagcattttt cttggattta gaaatgtaga attaacttca cttatgataa tgttatctgc
     3421 tccaactgca gtttcttcat ttacaatggc acaacagatg gattctgaca gtgaattagc
     3481 tggacaaatt gtagtattta cttctggatt ttctataatt acagtatttt taataatatt
     3541 tattttaaag cagaataatc ttatttaatg atatttgata acacaaattg gtattatgag
     3601 aaacatatat aaaattgatt taattgcgta aaaacatatt attatactat ttttactata
     3661 taatataact ttgtgtatat tatctcatta aacatggtga aagtagtgtt gctatatatt
     3721 ttttagtttg aaaaaaatat ataaatataa acaatatata aatacaaata ataaatgcca
     3781 acaaatagtc aatctataaa ataaaaactg gactaatcat ataaaatcat tagtccagct
     3841 tttattttac gtacttttta tgttgatgta cttttattaa aaactaaatt taatttttaa
     3901 atatctagca aatgcactaa ttaaataatg gccagttatt caacaatatc actacttctt
     3961 aactataagt aatgccaatg catttataaa aaataatact gctgttatct ttgtaactct
     4021 agcaagaaat gcttgttttc cttttggttt aaagtaagct tgacttcctt ctccaccaaa
     4081 ttctgaaggt aaagcatttt ttgaatcttg aggcattatg ctaattacta gtaaaatccc
     4141 tagcactact tgcacaccca ttaaaatgtt gctcattatt gcccctccta tctatttatt
     4201 acttaaattt cattactctt tagtgtatca caataatata gtcatttcaa gactattgaa
     4261 gtataaataa caaacaaaaa taatatgcaa ttactatatc ttttttgaat aacgtacttc
     4321 taataatttt tttccaatat gtatctcttt ctcaaaatca tcacaagtaa aaccattttt
     4381 tttatagaaa tttatagctc tagtattgtc ttttagaacc catatcgtta tatatgaata
     4441 tcctaaattg cttaatctat caatgcaaaa ttgtaatcct ttttttgtca cagatgttcc
     4501 ccaaaaatcg ggatggaaat atatagaata tatttctcca acacattttg gagcattatc
     4561 ctcttgactt ctatttaatg aagcaaaacc agcaggtata ccattcactt taaaaagata
     4621 atattcctct tcagcttttg gaataacact ttgaaaaact tctgctcttt tctcagaagt
     4681 gaattcatct attatcttat ctggaataat tcctttatac gcttttctcc acgaacaaga
     4741 atgtataaaa cccatttcta tagcattttc acttgtagct cgtataatct ctaaattcat
     4801 aaaaacacca tcctattgac aaattaatac gattatcgta ctatattagt gcgataattg
     4861 tatcatatat ggaggtaaaa gtcaatgaat cctgtaaaag agctaaacaa cctgtggcat
     4921 attgttagta tgcagatgaa aacagaaatg tgtagtggta agtatcctac tattcatggt
     4981 atttctttag tagaattaag tattttagaa atagttgaac agtatcccaa tagtatgatg
     5041 aaagaaatta gtgaaatttt agaattgcca aaaagcacat taacaagtgc tgtaaaacgt
     5101 cttgaattaa aaaaatttat taaaaagaat ccctgtatta aagacaaaag gaggtataac
     5161 ttagtcttaa ctgaattagg tttaaaggct caacaagaac atagagacat agaaataatt
     5221 gtatttagaa atctcttaca tcaattagat aaagatgaaa tagaaacttt tattcgtatt
     5281 ttttctaaag ctgtgaataa aagtaagaaa tgaaatataa aaaaaggata agtgaattga
     5341 aaaatcaact atcacttatc ccttataaga taaattttta tcttttatgt tatcttttta
     5401 atctttaatg ccatattttt aatcttcaat aactattttt ttaaattata gaaagatttt
     5461 aatccacagt atctagcttg ttctccaacc atttcttcta ttcttaataa ttggttgtac
     5521 ttagcaactc tatcagttct tgatggagca ccagttttta tttgaccagc atttactgca
     5581 acagctaaat ctgctatagt agaatcttca gtttctcctg atctgtgaga tataacagca
     5641 gtatatcctg ctctcttagc catttctata gcatctaaag tttcagttat tgtacctatt
     5701 tgattaactt taactaatat agagttagct actccatttt caattccttt ttctaatctc
     5761 tctgtgttag taacaaataa gtcgtcacca actaattgta atttatttcc taatctttca
     5821 gttaataact tccatccgtc ccaatcttct tcgtctaaac catcttctat agttattatt
     5881 gggaagttgt ttgaccaatc ttcatataaa gcaaccattt cagcagcagt taactctttt
     5941 ccttctcctg ctaaaacgta ttttttagtt tctttgttgt acatttctgt agcagcaacg
     6001 tcaagtccta acataacatc ttcacctggc ttatatccag cttttgttat agcttcaact
     6061 attaattcta aagcttctct gtttgaacct aagtttggag caaatccacc ttcgtcacca
     6121 acaccacaag ctaatccttt ttctcctaaa actttcttta atgaatggaa tacttctgct
     6181 cccattctta aaccttcttt aaagcaacaa gctcctactg gtaatatcat gaactcttga
     6241 acatcaacgt tattatcagc atgctctcca ccgtttaata tgttcatcat tggaacaggt
     6301 aattgtttag catttactcc acctaaatat tggaataatg gtaaacctat ttcatcagca
     6361 gctgctctag ctactgccat tgaaacacct aatatagcat tagctcctaa tttacctttg
     6421 tttggagttc catctaattc tatcatgatt gcatctatag ctggttggtc aaatgcatcc
     6481 attccttcta tttcaggtgc tattatttca tttacgttag caacagctgt ttctacacct
     6541 tttcctaagt atcttccctt atctccatct cttaattcaa cagcttcaaa agctcctgta
     6601 gatgctcctg atggaactat tgctcttccc attgctccat cttctaatac tacttcaact
     6661 tctacagttg gatttcctct tgagtctaat acttctctag catatactaa ttcaataact
     6721 gacatttgta tatctccctt cgattttcta ttagttaaat tttcatttac tgtaaacatc
     6781 cgtctactat tttaatctac tttctatatt atagtacata aagttgcttt tgaaaattag
     6841 gtacaaattt atttgatggg taaatttgta aaagttccta tttttatttt attttttata
     6901 atattttaat ttatataata taaaaaaact tgtctaaaat ccaaaa
//
LOCUS       DAEPLF010000179         6889 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEPLF010000179 DAEPLF010000000
VERSION     DAEPLF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.179"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(191..949)
                     /locus_tag="KR314_002908"
     CDS             complement(191..949)
                     /locus_tag="KR314_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBH0329277.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(951..1667)
                     /locus_tag="KR314_002909"
     CDS             complement(951..1667)
                     /locus_tag="KR314_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBH0329278.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWAGKMIYCIICCSIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLTNMFFT
                     IVGVAVFSTTEMWYVFPYSIPSRLMVVVLHILPNGLPVPEGSELLNNAVILPGVLISV
                     ILFVLFALVTRAWFNNREVK"
     gene            complement(1669..2376)
                     /locus_tag="KR314_002910"
     CDS             complement(1669..2376)
                     /locus_tag="KR314_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBH0329279.1"
                     /translation="MNNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRKLIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVIGYQGEVSKEQN
                     LEKLFMKVVAENRKRGE"
     gene            complement(2493..3029)
                     /locus_tag="KR314_002911"
     CDS             complement(2493..3029)
                     /locus_tag="KR314_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH0329280.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(3131..4201)
                     /locus_tag="KR314_002912"
     CDS             complement(3131..4201)
                     /locus_tag="KR314_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBH0329281.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDISVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSTSSYLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSNMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(4194..5252)
                     /locus_tag="KR314_002913"
     CDS             complement(4194..5252)
                     /locus_tag="KR314_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBH0329282.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNIKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSVTIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(5239..5571)
                     /locus_tag="KR314_002914"
     CDS             complement(5239..5571)
                     /locus_tag="KR314_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH0329283.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            5770..>6889
                     /locus_tag="KR314_002915"
     CDS             5770..>6889
                     /locus_tag="KR314_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBH0329284.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDNHYS"
BASE COUNT         2375 a         1135 c          726 g         2653 t
ORIGIN      
        1 cttaactttt caactttttt gttgttagat tttttacttc taagcatttg tgccatatcc
       61 tccaaatatt ttaattaatt cataattaat acctaaccag tttttatttg attattttat
      121 atttttcatt gctaagttat attttaacat aataatataa atataatgta aataaacagt
      181 caatttttat ttattcttct gactttttac cttcccattt agaaaaccaa atacataaaa
      241 ttacaaatgc taaaattgtt acaaaaccac acattaaacc tgttgattga tatgctttta
      301 ttgattcaac ctttatatat tcaaccgttt tagaacttga aaaagacgac caaatagtta
      361 taaatctaac accccatgca caaggaaaat actgccaaat gccagaaccc aatccagtta
      421 ataataatgc tgaaagtaac atttcaacta tacctactcc aattgatgtt ccttttccga
      481 accttaaact taagaataaa tgtaatatgt attcaaataa attacaccca attaaaatta
      541 gaattgaaat catataaaag cttgatgtaa agtaagcctt agatatacca aatttaaaac
      601 ctaatacaga cactaatgtt gctacaaaac caagacatac taagattata tatttactta
      661 taagagtaag atattttgga ttagatgttg ttaacatatg tttataattc cctgcataat
      721 attcttgttc aacaaacatt gaacacacaa cacctgacaa taaaggaaaa gctattgcta
      781 taacttgtaa atatgctact atctttaata tattatcagc tcctgaaacc ttataatacc
      841 ataaaaacaa tcccatcccc attaaagcaa tgcaaatgtg cattagtatt ataaaatttc
      901 tctttaattt tattaaatca gaacaaaata ttcttattag ttgtctcata ttacttcacc
      961 tctctattat tgaaccaagc tcttgtaact aaagcaaata atacaaataa aataacagat
     1021 attaatactc ctggtaaaat aacagcatta tttaaaagtt cactcccctc tggtacagga
     1081 agcccatttg gtagaatatg caatacaaca accattaatc ttgaaggaat cgaatatgga
     1141 aatacatacc acatttctgt agttgagaaa acagcaactc caacaatagt aaagaacata
     1201 tttgttagaa ctgtaacaaa tataccaaat tttgaagcta aaaacataca aagaggaatc
     1261 tgccacataa atagtaaaat aattaatata gttgcaatga tattttgacc tattgaaata
     1321 gtttctttat agattatccc aacaaaaact acacctattg cagaaataat cgatgctata
     1381 gaacaacaaa taatacagta aatcattttt ccagcccaaa cgtctttcat ttcaattggc
     1441 aatggtaaaa taccacgata tttacttttt tcatctttta atactattaa agaagatatt
     1501 aatgttaatg ctcctggaag aaatgtcgta taccaccagt tgaaagcagc attttgccct
     1561 ccacctagtc cacacaaaag tatacaaaac actggaacta tccatatcaa cttcattcca
     1621 aaagttcctt tcatcttttg aaattccact ctaataaatc tttccatatt actcccctct
     1681 ctttctattt tcagctacaa ctttcataaa aagtttttct aagttttgtt cttttgatac
     1741 ttctccttga taaccaatta ctccattaga aattattccg atttcatcta cgacttgttc
     1801 aacttcagac aagatatggc ttgataaaat aactgtaatt ccttgttttg gaaaagaacg
     1861 aatcaatttt ctaagttctt caataccaat agggtcaaga ccatttgtag gctcgtctaa
     1921 gattaaaagt tttgggtgat ttaataatgc aatagcaata ccaagacgtt gcttcatacc
     1981 tagagagaat tgacctgaac gctttttaaa agtatctctt aagtcgacag tctctaaaac
     2041 ttcttcaatt cgagagtcag gaagaccaag taaagtagta tggactttta aattttctcg
     2101 tgctgttaaa ttctcatata tagcaggaga ctcaataaga gaaccaatat ttgataaatc
     2161 ctttctagtc catctatgtc cctcaaacat tatttttcct gaagttggat ttaaaatccc
     2221 agttatcatt tttaataaag tagatttccc agctccattt ggtcctaata atccatatat
     2281 agaattagat ttaattgcta gtgaaatatt atcaactgcc atctgctcct taaatttttt
     2341 agaaactcct tttacctgca aaatataatt attcataatt ttcatccttt cttttgtcaa
     2401 acttatttac aagaatcatt ctaaatcata aatataagga ttttataaga attaaaaaac
     2461 aaaaaaaaga tgtgttaaaa acacatcttt ttttatctta attctattaa ttttccattg
     2521 tagtatatct ctacttcttc aatccaaatg ctattatctt cttcttggat gacattttta
     2581 ataatattat ttatagtata tattatattt tgttcattca aataatcatc tttccaagaa
     2641 actctacttt ttttatctgg ttctatcaag tttattttaa caagattatc tttttcctta
     2701 acttctactt ccatgtctaa accattaaaa tactcatttg ataatgtatc taagattaat
     2761 cgcagtttat ccttaagtga actttcttca tcaatctgta agtactttac tattttctca
     2821 tcctttgaaa ttatctctat aggtatttta gtagtttttt tagaattaat cattttacta
     2881 gttgtttctt cttttgcatc ttcatccttc tttacaactg ctgattcttt agaatcaact
     2941 agctcaactg cactgatgct ataatccata catgctgtac tcaataaagt agtagcaatt
     3001 attaaaaaac ttactgctat tttattcata agtatccctc tcctaattct ctttcgatac
     3061 ttattatacc atagtataga ttatattttt atacttttaa atcaatttat aaattattat
     3121 agtaaattat ttacaattct aagctatttg aatttatttt tatagtatat tctttctcta
     3181 aattcgacag atttttattc aaaagccctt ttatttccct cgtatttcta tacttgttgc
     3241 tatgttcttc actatgttca taatcatatg aatcattgtc atattcttca tcattattaa
     3301 attcttcaat tgcttctttt gactttatat cacaattaaa tttatactta tttactggta
     3361 tatcaagtct taccttagaa tttggaactt ctatgtccag gaacttagca attggcatat
     3421 tagagtacaa gtaaaattca ctttcataat ttgaatttct acctattttc tcttttatat
     3481 ttacattttc aggtatatac tcttctatat tattgataaa tacatcttca ttctctgaag
     3541 atatatccaa tgactctgat tctatgttta catttttaac gcctaaaatt gattttgttg
     3601 acaagtgaag attgctatta gatattaagt ttaggttttc taatctattt actttgttct
     3661 ctgttatagc tctactaaaa cctccataag aagttttata tgttatagta tttgaagcta
     3721 cattatcata tacaaataag tatgaacttg tacttgatgc atttatgtct acattatttg
     3781 gtacatatat tataatttct ctataatcat gtgaaaacat actatttata gatgtattta
     3841 ataattggtc gaaattttta atctttttaa ttttattttc atattttctt tcacctttta
     3901 caactaaagt cttatctttt aaagttacat tgtatttgta aaactcatta tttcctcttg
     3961 ttgttacacg cacatcttca cttgaacttt tctttattgt aacgtttata ttgtcaacag
     4021 atatatctaa cttgtcaata tttacttttt cttgataaag tttattttct tttttaaatt
     4081 cagcttcctt ttctaaacca aagtttatta ggcttggcat aactaaaatc cctgaccatg
     4141 ttgtccctat tatacctatt accaataaaa ctgcagcaaa tatagcaagt tttttattca
     4201 tgtttatctt ctcctttctt attttctata tcttcaaggt ccttatatat cgcctcaaaa
     4261 tttatactat catcattata ttcatcagtt tcttttatgc tttcttcttc tctattgtag
     4321 taatcattat tatatgaatc ttcattaatg tctttattca aatcttcatc atttaaagta
     4381 gctttttttc ttttttccat cttttcattt gcctttatat aaatattttt tctctttatc
     4441 caatgtatat aattatttag taattgttta cctagtttta tagcaaataa taaaatttgc
     4501 catgcaagta tctcaagccc tacaaatgct atggttgcga aaatacaaaa catcataaca
     4561 tctggaacca ttgctataaa tttaatcaca aatggaatta atataacaaa agcaattaat
     4621 gaacatacta atgcaatagt aacagataca atagcacata caaacgcaaa tgctggaaca
     4681 attagaaata aagataacac tactaataat ccctttataa cttgtctaga taatccccct
     4741 acaccttctt ctatacttgg tgtaagttta tcatttatat tttcttttga tttattgtac
     4801 ttttccttag cttcatcttt caagatagag taatcctttt ttatatttga tttaaaccta
     4861 ctaaatttac catttaatat atctgtattc attatttttc catctatatt ttcttttgat
     4921 ttactatact tttcttttgc ttcatctttt aagatagagt aacctttttt catatctgat
     4981 ttaaatttac taaacttatc attcaaaaca tctgtattta ttattttttc ctcttcaata
     5041 tttttttctt tagtctgcaa gataagttct ttggctatag attttggtga accaagtgat
     5101 gctattatct ccatatcact ctttccctct ataagaccat ctacgaaata ctcttcataa
     5161 tctctcaata tatcattaac ttcgctctca gaaaaatctt tttttagata atcctttaaa
     5221 atttcaagaa actcagtttt attcaagttc attacctccc tcatcttcac ttattaaaat
     5281 ctcaacagcc tttacaaatt ctctccactc ttccatcaga ttggataaat tctcttttcc
     5341 tagtgaagtt attctatagt attttctagc aggaccttca ttagattcta gtatatatgt
     5401 ttcgaaatac aaatcttttg taagtcttct tagaatagga tatattgttc cttcatttac
     5461 atctattact ttagagatgt tatgtactat ctcatatcca tacatatctt tcttagatat
     5521 gagagcaagt acacaaattt ctaaaacacc ctttctaaat tgtgtgttca tatactccat
     5581 cctttcttta aatacacagt attgtgtttt acaaggtact atgttaaaac ttcttatatc
     5641 ctcataataa cacagtacta tgttctgcac aatagttatt tttaaatatt cttgttgatt
     5701 aaatccttct ataaatgtat aatactctaa tcagatatat tttatgaaag tggtgataaa
     5761 ttggctaaaa tgaaagatga taaaacaata ttaatcttaa ctgctcaatt tggtgctgga
     5821 catataagtg ctgcaaaagc agtcaaagaa tgtattattg aaaaatatag taactataat
     5881 gttgttatac aaaactttat aaatgcaagt attcccatga tgaataaacc tatggtaaaa
     5941 ctttatgaaa acaatacaaa atatactcct ggattatata attactatta ttactttaag
     6001 aaatcgtttg attcaagaca tgatttatct cataaattat atacacctaa actttctgag
     6061 tatattgctg atataaatcc agatttgatt atctctacat ttccactagc ggctgcttgt
     6121 gtaaataact ttaagataaa aaatcctgat ataaatatac ctacacttac agttataaca
     6181 gatgttgtag atagtatgga atgggttttt gaaaatacag atttgtattt tgttccttct
     6241 cctgaaataa aaaatagatt ttttcagaaa ggaataaatc cagactccat aaaggttact
     6301 ggagttcctg ttgataagag atttcaaatt gaaagtaaag aactatgttg tgataaatat
     6361 agactattac ttctaggtgg aggaagaggc ctatttgata tagatgagga ttttatgcat
     6421 tggatagatg aatttattga agaacatagt aattctatag aaattacaat agttactggt
     6481 aaaaacaaaa aattatatga caatctaact cataaaaaac ctctaaaaaa tattaaagta
     6541 cttggatttg taaacgacat gtataatcta ataagagaat gtgacctaat gctaactaag
     6601 cctggaggag caactatatt tgaagctatt caatcacaaa ctccagtact cgtgaaaatg
     6661 ccaaaagttg gacaagaaat tgaaaacgct aaatttataa tagataaggg tcttggcatg
     6721 atttatagtg atgacctaga ccttaaaaac atattttata aattagtatc aaatgaattt
     6781 gattctataa taaactttat gaaaaaaaat ttagaagaat ttaaaacagt tattcatcca
     6841 gagaaaattg ctgattatat atcagaatta atagataatc attacagtt
//
LOCUS       DAEPLF010000180         6839 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEPLF010000180 DAEPLF010000000
VERSION     DAEPLF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6839
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.180"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..796)
                     /locus_tag="KR314_002916"
     CDS             complement(<1..796)
                     /locus_tag="KR314_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0329285.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVNYPNLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNDEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMG"
     gene            complement(1166..2068)
                     /locus_tag="KR314_002917"
     CDS             complement(1166..2068)
                     /locus_tag="KR314_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0329286.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDGQISFFD"
     gene            complement(2118..2579)
                     /locus_tag="KR314_002918"
     CDS             complement(2118..2579)
                     /locus_tag="KR314_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBH0329287.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(2631..3086)
                     /gene="def"
                     /locus_tag="KR314_002919"
     CDS             complement(2631..3086)
                     /gene="def"
                     /locus_tag="KR314_002919"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBH0329288.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(3058..3321)
                     /locus_tag="KR314_002920"
     CDS             complement(3058..3321)
                     /locus_tag="KR314_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329289.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(3418..4134)
                     /locus_tag="KR314_002921"
     CDS             complement(3418..4134)
                     /locus_tag="KR314_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329290.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGTLAFIITMILLIFFSLTKISFLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYIYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(4131..4808)
                     /locus_tag="KR314_002922"
     CDS             complement(4131..4808)
                     /locus_tag="KR314_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329291.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILISIIIIIYFITYYFICSSWK
                     KVGGVKL"
     gene            complement(4805..5650)
                     /locus_tag="KR314_002923"
     CDS             complement(4805..5650)
                     /locus_tag="KR314_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329292.1"
                     /translation="MIKVDDLSFSYTDRNFLQNINFEVEKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIKKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(5764..6354)
                     /locus_tag="KR314_002924"
     CDS             complement(5764..6354)
                     /locus_tag="KR314_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0329293.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRVVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            6643..>6839
                     /locus_tag="KR314_002925"
     CDS             6643..>6839
                     /locus_tag="KR314_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBH0329294.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLY"
BASE COUNT         2422 a         1082 c          641 g         2694 t
ORIGIN      
        1 atcccatagt catactatcg tcagaatatg cttcaccctt aacattacta aaaaactcaa
       61 cagacttaaa tccaatattt tcaacttcta ataagaaact ttcttttgaa aaatagtgat
      121 tccaaacata aaatacattt tcatcttttt cggttattac aagagtttgt tctaatgtat
      181 tataatcatt gtatttacaa ttatcttgta gacacatata tttttcatta ctccaaaatc
      241 ctccatcttc atttatttcc caatatttaa tttcctcaaa attattgtat ttatttattg
      301 taaatacatc taatattaat tttcctccag gttttaaact atcataaatt ttttccatta
      361 ataaccttct attttcagtt gacaaagctc cataatcaca ataaattaaa gtagccaaat
      421 caaattcatc attatagttc atatttaaat aactttgaaa caagtaattt atgtttaaat
      481 tagtctcctt atttctattt tgtgcatagt taatagaacg ttttgagaaa tcaattccag
      541 ttaccttata acctttttgt gcaaatttct ctgcatataa acctggacca caacctaaat
      601 caagtaaatt aggataatta actggatttg caactgtaac tatccaattt acagaatcct
      661 cgataaagtt aaaatttcta cttgctcctt caaaattagt gtctaaatgt gcttttaata
      721 attgttttga aatatactca tcatcccaaa aattaatttc actttgttta taaagctctg
      781 gtctttctaa atacatacat aatttgttta acaaaacata ccactcctta aaatttattt
      841 ttaaaataaa aaaaccacag agtttactct gtggtttaac ttcaaaaaga tgagtttaaa
      901 taagagtaaa atagaaccaa atatttttaa gcacaacaat aaaactatag ctttactttg
      961 tttgcttatt aaatttgatt ttattcactc ttttccagca atacttatat caacgatttt
     1021 atgatatcaa atatcaccga acaacatcat cctttcatat ttttatttta tgaatcaatt
     1081 atatatttaa attttatatt tgtcaataaa ttttaatttt aataaatttt aatcatcata
     1141 gtaaaaaaaa attttttata taaaactaat caaaaaaaga tatctgtcca tctcctgagt
     1201 tttttaactt ttccttcatt attttagagt aactgcttga tagagaatat ttatatctaa
     1261 gttcattaac cattttatac aattgatttt tatactccct attaagtgaa cctgcactat
     1321 atagtattga taatttatca aataattcag gaaactcttt tttaacaaag tcaaaaaaaa
     1381 cacctctagt tatacccctt aagtataaag tcccaggtaa aacataatgt acattacatt
     1441 cttttgcatg tctaaatagc gaatttatat tatcaaaatc atctgtaata tatggtataa
     1501 ttggcataat atgtagacca actgatgcgt tagtttttct aaattctttt aacattttaa
     1561 atcgtctcat tgaatccaca ccatttggct caataagcct ttgtgtatct tcatttactg
     1621 tggtaattgt agatgctaca ttgatatatg taatcctaga tagcttatca attaaatcgt
     1681 aatctcttaa aattaaatct gactttgtag aaataatagc tggtgtcttg tattttatca
     1741 gcaggtttaa tatttctggc ataattttat agtcagcttc tattggttga taactatctg
     1801 taactcctcc tatattgatt acttctttct tccatttact gcttttaagt tgtctctcaa
     1861 gtttttctac tatatttgtt tttacataaa tctcttcaaa ataattattg gagttaatgt
     1921 acttatgaga gtaaatagca taacaatatt tacatccatg ctcacatcca cgataaatat
     1981 ttaaatccca tttatatggc atactccttt ttaatttatt acaagctatt tcacaattta
     2041 ttggaatata ttttatttta ttatacaaat aatcacctct ttatatacaa taatactata
     2101 atgttaatct atagtaatta aaacatgata tttccagacc acaattcata atctttacta
     2161 tatttacaaa gtaatatatt ccattcattt attggtattc ctccacatgt tgctttttct
     2221 tctaaattat ataagcattg ctttaaatac attttattat gccaatcatc atataaattt
     2281 tctaaattta aactatgttc attagatata tcatttgcaa aatttctatt tttccatttt
     2341 aatatacttg tatatttaag ttcattaggt ttataatttc tttttaacat ttcatcatgt
     2401 acaatttttg tataaaattt aaattcatct atactataat tcaatacttt attcactaat
     2461 agatggtttg gagtaccttt tttatctata gaacctttta tagcaagtag ctctctccat
     2521 tgacttacta attgattttt aggtaataca tcaattaaat ccttatgcca taatctcata
     2581 atttaactcc ttactataaa atattttata aattcaataa ccaatctatc ttacaatttt
     2641 acatgttcaa taattttatc tgtaaaaact ataccattta aatggtctat ctcatgacaa
     2701 agacattttg ccataaaacc tgaaccttta atttctattt tctcaccgta ttcgtttaga
     2761 gcttgcactg taactttttt tggtcttttt aatttacccc atacttctgg aaaacttaaa
     2821 catccttcaa caacaatttg ttccccttct tgtttaataa tttcaggatt aacaagctta
     2881 ataagtcctt ctcctaaatc tataacaact aatcgcttta atactcctac ttggcatgca
     2941 gctaatcctc ctccatttgg agtattgtac atggtctctg ccatgtcatt taatatttct
     3001 cttatcttat tatctacgtt ttctacatac ttactctttt ttcttaaaat ttcatcatca
     3061 aatgttctta tctttcttat agccataata ttctccccta cttatctgtg ctcttgattt
     3121 tctttcactt tcttcaaata aaaatacact ttcaattggt tcattaaaaa ttcttgaaat
     3181 atcatatgcc aaccatattg aaggttcatt tttaccagtt tctatagcat ttatagcctg
     3241 tctagatgtt ccaactaatc ttcctagttg ttcttgtgtc aatcctaatt tttctcttaa
     3301 tatctttatt ttatttttca tatttgctcc attcatgtaa ggttaacttt acatcttaaa
     3361 tgtaacattt ttcaacatta ctgtcaagtc aaccttacat ttatatatta aattttctta
     3421 tttaactatt tttttactaa actttttaaa taataataaa atccatatta atgatataac
     3481 aattgaaata aacatataga tataattatc ttccttaaat gccttcgata tccaaaaagt
     3541 tggtaagaag aacagaatat attgtgcttt atttaaaata aaaaatggtg ctaaaatacc
     3601 catcataaaa acacctgaga atttcgtaat taccattcct tctagtttat ttgatgataa
     3661 agaaatcact agtattgata taataactcc ttgtaataaa actaataaag aaattgataa
     3721 gtttaacaag aaagaaattt tagttagaga aaaaaagata agtagaatca tagtaataat
     3781 aaatgctaag gtacctggta ttccaaatcg tgaaataaga taaccagttt taccaagcgg
     3841 agttactatt aaatatcttg aaatgctgtc atcaatttct cccaatatga ccattgtaga
     3901 agcaaaaaag aacataaaag gtgtaataaa agctaaaagt aaatcaaaca tcaagaaata
     3961 cggctctaaa tttaattgat aataaaactt gttaagtgat atattctgta ttaatggtat
     4021 tccaaatttt ataaatactc cacataatat tggagaaaca catactataa aaagcatagc
     4081 atctctctta atttgttcaa actcttgttt aaacgcattt ataatcttta tcatagtttt
     4141 actccaccta cctttttcca actgctacaa ataaaataat aagttataaa ataaattatt
     4201 attataattg atattaaaat attaatcatt ataaaatttt tatctccact aataagagaa
     4261 atacatatat taaaagggta caaatttgca tatgattttg tgtcaagaag tacattgagt
     4321 attggtggaa taaagcatat aatctcaata ggaatactta aaacaataaa ctgattcaaa
     4381 ctagatgctt tacttgcaag tatcaatcca agcaaagaaa atataataga agaaaaaaag
     4441 gtacctatag ttgatattat aatattatct aaatttaatg taatagcaat aaacatagct
     4501 acaatactag atattactcc taatgaaatt acctttgata aaatatactc agatactttt
     4561 actggagata cagctaagga atttaaaacc ctttgacttt tctcaagaag tacaatagct
     4621 cccataaaaa acaatcccat tgctgctggg tcagaataaa tcataattat agctattttt
     4681 tctctcataa aagttggaaa tacattgata atacaaatat atataatact taaaaaaaga
     4741 tataaaaaat aaaaaccata cttaaattga aactttatat ctcctaaaat aagatttttt
     4801 agcctcattg taaattcctc cctgttatct caataaaaat atcatttaat gttggttcac
     4861 tactatgtat tgataaaagt ttatttttct taataagcat attcaaattt ttatcattag
     4921 cagtattgtt cagaaaacat tcagaagttt tttctccatt atcaaagtaa gtatatctaa
     4981 ctttaatagc tccttttgac ataataagat tatgtggtgt atctaaagca gatattttac
     5041 catttacaat gaacgctact ctgtcacaaa gctccgttgc atctaacatg ttatgagttg
     5101 ttaaaataat agttttacct ttagattttt ctgataaaat tatatctttc ataaccttgc
     5161 tatttgacgg gtcaagacca cttgttggtt catctaaaaa taaaatatct ggattatgta
     5221 ataaagcttt tataaaattt aatcgagatt tcataccttt tgaatactca gatacccttt
     5281 tatttgcttc attttctagt cctactgatt ttaataactc atcaatagat agtagttttt
     5341 tagaatatag agacccaaaa tattttaaat tttctattgc agtcaacttt tcatataaac
     5401 taggaaattc aaaatcaact cctatatttt cataaaattt atttgagtgt cttttactct
     5461 ctactccatt tacaatgaca cttcctccat aattagttat cattccaatt agaatctttt
     5521 gtaatgtaga ttttcctgca cctgatggtc ctaaaaatcc tagtatttcc cctttttcta
     5581 cttcaaagtt aatattttgt aaaaaatttc tatctgtata actaaatgat aaatcatcaa
     5641 ctttaatcat tcaatatcac cctttcaaat ttttgactat tttgactata tggtcaataa
     5701 aatataattt ttttgtttgt taataatttt taataaaagt tattagcaaa caaaatttaa
     5761 attctattcc ataagttgta taactaatcc gtttatcatt aatcttaaag catcttcaaa
     5821 acaattactt ccaacctcgc gcttatatag catggtaaga aaaactactc taaatgctcc
     5881 actaaagctt tctatgtctt tattttttgc atttggtatg caagatataa tctctccaat
     5941 ttcaaagtca tcatgtctaa agtgttcttc aattatagaa tctggtaact ttctcaccaa
     6001 tatctcaaat tctccattta tcatgagatt taaaattgaa gtactatcaa ccatcctata
     6061 gaatttcata aatatatctg tcaattgttc acatgtaata ctttcatcta ctctatttat
     6121 ctcatataag atttcttttt gtatagagtc atgtatatct ctcaatacct caaaaaagag
     6181 caattctttt gattgaaaaa atagataaaa agttcctttt ggtattttta cccgttttac
     6241 aagctcatca acagtagttt ttctaatacc atattgctcc atacacaatc ttgcttcttc
     6301 tttaagtctt tttattatat actctctttc tttatcagaa tacgatttag gcatcttttt
     6361 cctccagttt gactaaaata atttatatag tcaatataat actttttcta tctaatgtca
     6421 atagtattat tagtatattt aaaaataagc ttaaaaactt tcgaaaattc tgaattttat
     6481 atagcatttt ttattatcta gtgttataat taagatataa ataatatatt attctaattt
     6541 tcatatctat ttgcttattt tttaacttag tttctaatag aagtactcat atttatcttt
     6601 ttaaaagttt caactttaat aaaaaataaa ggagttgatt ttatggctac tactacaaaa
     6661 aaccgttttt acgattatgt tcaagaaaac ttaaataaat atgttgaagt acattcttac
     6721 tttagcagtt gtcctctata tggtgaaatt atagaagcag atagcttatc aattacactt
     6781 tgctcaagat atatagatga atctaatcca actttaaatg aaacttacac attatattt
//
LOCUS       DAEPLF010000181         6772 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEPLF010000181 DAEPLF010000000
VERSION     DAEPLF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.181"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(94..579)
                     /locus_tag="KR314_002926"
     CDS             complement(94..579)
                     /locus_tag="KR314_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0329295.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            831..2054
                     /locus_tag="KR314_002927"
     CDS             831..2054
                     /locus_tag="KR314_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBH0329296.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTKESEPEKPIAVSVQKAVGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNANNKFKAGMSAQVNLSIEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            2058..5192
                     /locus_tag="KR314_002928"
     CDS             2058..5192
                     /locus_tag="KR314_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBH0329297.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            5226..6524
                     /locus_tag="KR314_002929"
     CDS             5226..6524
                     /locus_tag="KR314_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBH0329298.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINNVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNQDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
BASE COUNT         2650 a          890 c         1154 g         2078 t
ORIGIN      
        1 aatagcagta gtataataat ctatattata ttactgctat ttcttttctt atgaagtttt
       61 aactttttta atagtgactt ttacaatcac actctaacct aatttcttta ctatttctga
      121 taactttaat gtatgttcat ctaataaagt tagttcctct tcagaaagat tagataattt
      181 ttttactaga gattctttga gaatagcttt tactgcatca aataatttaa atgctttatc
      241 tgttaattca accctaagaa tccttctatc ttttgggtca ggaaatttat tgactaaacc
      301 atgttccact aatctgtcaa taatcggagt catatttgat tttaaaattc ctaaattaga
      361 agctatttga gaaattgatg atgaatttga tgtcataaga taaagaatta ccttagcatg
      421 tgatggagcc attggacatt cttcaaaata agctttcatt atatcagagt tattttgaaa
      481 cttttttaac aaatcgcttt ctttaaatat attattttca agcacaaata aaaaatctaa
      541 aaaagagtcc gctattttaa gtatattgtt gttatccata tttgtaacat cccttcaata
      601 gataatataa taaaacattg ttgtttaata tagtataaat tataaactat taataaattt
      661 aaaacaagaa aaagttattg actcataata attataaatt tataatcaaa ttagagaatt
      721 ataaattgcc aataaaggga agttttttaa ttagtctaaa actaaactta aattataaga
      781 cactttaaat tctacaacaa gctagtgtgc aatatataat aggaggagat atgaaaagat
      841 atttaatagt tttcttaatg tgtagttcac tgtttttagt tggatgtgga aaaacaaaag
      901 aatctgaacc tgagaaacca atagcagttt ctgttcaaaa agcagtaggt ggagaaattg
      961 aaaacacaaa ttctttctca ggaactacaa aagttaaaga tgaaacagct gttactgcac
     1021 agactgttgg cacagttcaa gaagtgtatg taaaacttgg gcaaaatgta agaaaaggag
     1081 atgaattatt gagtataagc agtccagagc tagaaaatag tgtaaagcaa agtaaagcct
     1141 cactagattt agcaaaagca agttattcaa gtgccactgg tggttcatta gaggcacaag
     1201 taaatcaggc aaaaacagct cttgacaatg caaagattca atatgaggag tctcaaagaa
     1261 attatgacaa taataagatt ctttatgaac aggaagtcat tagtttagac caatttaaaa
     1321 aaatagagtt ttctttagag caaacaaaac aacagttaga ttctgctcaa agagcatacg
     1381 atacagcaac ttcaaaaagt atacctcaag ctaaagcact tgctaaaaaa cagcttgacc
     1441 aagctcaagt atcatataat ttagcaatga gtaatttaga caaattaaca ttaacatctc
     1501 cagtagatgg tacaataaca gctaaaaact ttgattcaaa ggaaatgata acacaatcac
     1561 aaccagcttt tattatttct aatcctaata tactagaagt tgatttaaat gtagcagaat
     1621 cagatatagg taaatttaag aaagatggaa atgtagatgt tataattgaa gaccaaagga
     1681 ttttaggaaa aatagattat gtaccttcag tagttgaccc tcagacatct ttatatcctg
     1741 ttaaagtact tgtaaataat gcaaacaaca aatttaaggc aggtatgtct gcacaagtaa
     1801 acttaagtat tgaaaaagaa aatggtgctg ttacagtgcc taaaaaagca atatttgaag
     1861 aaaatggtaa aaaatatgta tacatagcaa caaaagacaa tatagcaaag aaacacttgg
     1921 ttcaaactgg aattgtaaca gaggataaaa tagaaattaa aagtggtgta agtgataaag
     1981 atacagtgat tattggagga ataagtctta tttctgacgg aactaagata tttcctgtag
     2041 aaaaggagaa gtagactatg aatttaacac agacttccgt taaacgtcct ctcacaatca
     2101 taatggtctt tttagtatta atagtttttg gtggtattgg atataaaaaa atgtcaataa
     2161 atctaatgcc tgatatagaa ataccagttg taatggtcat gactacttgg acaggtgctg
     2221 gtcctcaaga cgttgatgaa caagttagtc aaaaagttga tgaaagttta tctgccgttt
     2281 caaatgtaaa atctactata tcaagttcac aagaaagtgt ttctatggta gttgctcagt
     2341 ttgaatttgg aactaatttg gatgaaataa tgaatgatgt taggtctaaa gttgatgcat
     2401 tacagaccag cctcccagat gatgcagcaa aaccaacagt tttaaagctg gatatgaatg
     2461 cacaggctat aggtcaactt gtcatatctg gaggaaatga aaatagttca caagccttaa
     2521 gaaaatacgc tgaagatgta attcagccta aaatagaatc catagatggt gttacttcag
     2581 cagaccttaa aggtggagaa aaagcacagg ttaatgtaat agctgaccca gctgttcttt
     2641 caaattatgg agttagttta tccactataa aaggtgtctt atcctctagt aataaaacat
     2701 tcccttatgg ttcaattact caaggtgagg ataaaatagt tttaagagca attgataaat
     2761 tagaatccct agatgatata aaacagattc aaattcctgt aaaaggagga aatacagtta
     2821 gattagataa tctttgtagt gtagattatg gatatgcaga caaagatagt atttatagat
     2881 acaatggaaa agacagtttg gtaatagata tttctaaaca acaagatgcc aacactgtaa
     2941 aagttatgca gagtgcatac aaatacgtag atgaattgaa taaggaaaat cctcaattta
     3001 aaattaaaat tgctaatgat acaagttcat acataagtga atcaataaac agtgtaatga
     3061 gcaacttgct tataagtgct gttatcgcat ttattgtaat atttgctttc ctaaaaagtg
     3121 taagagcttc actagttgtt gcagttgcaa taccaatttc tataataggt gctatagcaa
     3181 ttctctactt tactggtgaa agtttaaatc ttattactgc aagttcattg gtaatttcag
     3241 ttggtatggt tgttgataat gctacagttg taattgaaaa tatatttaaa tataggaaaa
     3301 gtggcagatt gagtcttgat gattgtgcta ttgagggaac aagaactgta acaaatgcaa
     3361 tacttgcatc aacattgacc acgatagcaa tattcttacc tatcatgttt acagaaggta
     3421 tagctaaaat aacttttggg gcattaggta aaacactaat agcttcacta gcattctcct
     3481 ttataggtgc aattacactc gttcctagtg tatttgcaaa attaagcaga ggaaaaaata
     3541 gtcaaaagat gcaagaaaaa gatagtccta tatttgataa ggtaagtgaa gcatataaaa
     3601 aattattaag tgtatgttta agacatcaac gcttagttgt aatatcaagt ttacttattt
     3661 ttgtagcttc cttatttgga atgggattta taggtatgga ctttatgcct gcagcagaca
     3721 agggtgaatt atctatttct atagaacttc caaaaggtct tagcctagaa tccaatgatt
     3781 actatgtatc tatgactgag aaaaaagtct cagatattcc agaaattaaa actttaataa
     3841 caactttgac atcagattca aataatagta aagcaagtat agcaattgaa ttagaatctc
     3901 aaaaaaatag aaaaagaact actgatgaaa tagaaaaaga gataattgat agagtggcta
     3961 ctgtaccaga ttgtaaaata aatgtaagtc aaaacagtag tgttatgggt ggagctggag
     4021 gagcagactt cacattagaa atgaaaggtc ctgatttaga tgtattaaaa atactgggaa
     4081 aagaagctga aaaagaattt agctctataa aaggatttag aaatgttgaa acttctctta
     4141 cagatacaac acaagaagct caatttacta tagataaaca taaggctcaa gaatatggtg
     4201 taaatactgc tgaaattgca ggcatgttac gtacagcaat aaatggtgac tccgtaacca
     4261 ctgctactat tgatgattac aaagtggatg taaacctaaa atttaaagca gatactattg
     4321 atagcttaga agatataaag caactaaaag taaactctgc aactggaaaa gaagttcctc
     4381 ttggtgcttt ttcagatata aaaatggcag atggtctaca atctatatct aggactgatg
     4441 gagatttttc tgtaaatata actgctacac ttgacaattt agatgctggt actgctacaa
     4501 aactagcact agaaaaaacg aataaaatgg taattcctaa agattactct ataggtgttg
     4561 gtggtgaaac agaaatgatg aatgaatcta tggcaagtct tggattttca atggtcatag
     4621 ctatagcatt agtttatatg gtaatggtag ctcagtttga atcatttagt aaaccattta
     4681 taatcatgtt ctgtgttcca tttgcattcg ttggagttgt gctttcgctt ctagttagtg
     4741 gtcaaagttt aagtactgtc ggtatgctag gtataatctt acttatcggt attgttgtta
     4801 ataatggtat agttcttatt gactacatag agcaacttag aaaaacaaat cgtaacaatg
     4861 atttaattgg tttggtttca attggttgtg cagcaagatt aagaccagtc ttaatgacta
     4921 cagcaacaac aattttaggt atgttgccaa cagcactatc tcttggtgaa ggtggagaaa
     4981 ctatgcaacc actagccata gttataatgg gtggtctaac tgtatctaca cttataactt
     5041 tggtgcttat acctactata tacttaatat ttgataaatg ggaaaataag ttttcagaca
     5101 aaatgcctaa aatatttggt aagataacag ataaaatagg aagtataaaa cgtaaaataa
     5161 taaagaaacc aaaattgaaa gctaaagttt aaatagaatg gagggtgatt gtattgaata
     5221 aaaaaatgag caaaatagtt gcaattggct gtggtgttgg tataataact gccaatttaa
     5281 tcccagttta cgcagataat aatacttctt ctataaacaa cgttgataat tctattttaa
     5341 gtgactcaaa taaaagaaca tctgaaaagg aaattttgac tgctagagaa gctgttagtg
     5401 ctgcacttaa aaatagtgaa aaattaaaaa tgaaatcaga agaaataaaa atgttaaaag
     5461 aaaagttaga agtacaagat gagtttgata gttttactgg aagtgacaac tcgtttccat
     5521 acgaccaaat tggacttctt aaaaatcaaa gtgaacaggc taaaggcttt atggaggacc
     5581 aaatagcaaa tgatattact aataaattta acgatttagt ttcaagagaa aatgagctag
     5641 ataagataaa aaacaatctt gaaataaaaa ctaaagaaat taaagatatg aaattaaaaa
     5701 aagatttagg gttagtaaca tctttagaaa ctgaatcagc tgaacttgaa cttcaaacac
     5761 tacaaaatac tcaaaaagct aaacttcaag aactaaaaaa caatcaagac tattttaagt
     5821 tattgactaa tatagattta aataattatc aattggataa agaatataga tttgaatctt
     5881 ttagagtagg tggctcagtt gattcatata tggaaggtaa ggttaatgaa tatttaaaat
     5941 acgaccaatt gattttagaa cgtacagaag aaagctttaa tgataaagat gagaacaaag
     6001 cagatttacc agatagacct aatcttacaa gaccagttgc tcctacaaaa ccagagcaag
     6061 gtaacctaac tgatgatgaa tacaaggttc ttatggataa gtatgaaaaa gactacaaga
     6121 gatatgaaaa agaagttgaa gcatacaatc ttgaaagaca gacttatgct gctggcctta
     6181 ctacttatgc aaattatctt caacaaaaat ttaataccga aaatggtcta gtaacattag
     6241 aagacagtaa aaaagctctt aaaaaagggt taattgatag ctatgctcaa ttacttgctt
     6301 tagaagatac aattcagatt actaaaaaac aacttgattt atcagaaaaa caattaaaaa
     6361 atactaaatt aagatatgat ttaggattaa ttactctaac tgattataaa aaacaagtgg
     6421 taagtaatca agatgctaaa aatagctatg acactcttat tgtgaattat aactcactaa
     6481 aaaatggtat agaaaaacct tggatactta atactggaaa ataattctgg taaatagaaa
     6541 gattacttta acatacgttt gattaaaaaa tagttatgca cactcgtggc tttagtcatg
     6601 gaagattcag aaggcaaata atagaaatag attagtaaaa atatatttac ttataaaact
     6661 gtatactttt atagaggcta tctcaaaatt ggatatgtcc ctgtcaagta gacagactta
     6721 aaaaaggcta tctttatgcg gaatgagttc gatattcaat cgaactcatt cc
//
LOCUS       DAEPLF010000182         6719 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEPLF010000182 DAEPLF010000000
VERSION     DAEPLF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.182"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            40..282
                     /locus_tag="KR314_002930"
     CDS             40..282
                     /locus_tag="KR314_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBH0329299.1"
                     /translation="MQNSMKQEPCDFSHGRFRFKTSDNRVLIPVVIFLGSIVTALCDL
                     ISRVILSPVELPISTITSLLGAPIVVMLLVKRKDRA"
     gene            285..1481
                     /locus_tag="KR314_002931"
     CDS             285..1481
                     /locus_tag="KR314_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329300.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(1601..2326)
                     /locus_tag="KR314_002932"
     CDS             complement(1601..2326)
                     /locus_tag="KR314_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBH0329301.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGANFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTPFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            2804..4903
                     /gene="nrdE"
                     /locus_tag="KR314_002933"
     CDS             2804..4903
                     /gene="nrdE"
                     /locus_tag="KR314_002933"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBH0329302.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYLKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            4916..5887
                     /gene="nrdF"
                     /locus_tag="KR314_002934"
     CDS             4916..5887
                     /gene="nrdF"
                     /locus_tag="KR314_002934"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBH0329303.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKKMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            5963..6589
                     /locus_tag="KR314_002935"
     CDS             5963..6589
                     /locus_tag="KR314_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBH0329304.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIIEIPEQKVIMLKGSGDPNSKEFI
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         2642 a          739 c         1174 g         2164 t
ORIGIN      
        1 ggggatggcc tgtggagaat tagtaagaca tattttagta tgcaaaattc tatgaagcag
       61 gaaccctgtg actttagtca tgggaggttc agatttaaaa catcagataa tagagttttg
      121 ataccagtag taatattttt aggcagtata gttacagctc tttgtgattt aatatcaaga
      181 gtaattttat caccagtaga attaccaata agcacgataa catctttatt aggagctcca
      241 attgtagtaa tgttattggt gaaaaggaag gatagggctt agacatgttg aaaactaata
      301 atttaagtgt aggatacaat aacaaggtag ttataagtaa tataaatgta gaagtaaaaa
      361 atggagaaat actatgtctt ttgggaagta atggagctgg aaaaactacc ctattaaggt
      421 ccttatctaa acttatatca ccaataaaag gtgaaatata tttgaatggt gtaaacataa
      481 attgtatatc aagaaaagca ttatcaaaaa aaatggcttt agtacttaca aatagattat
      541 taggtgattt aatgactgta caagatatag tcaatatagg aaggtatcca tatacaggat
      601 tttttgggag tctatctaag aaagatttaa taatggtaga tgaagcattg gagtctgtag
      661 atgccttaca tttaaagaaa agatattttg atgaactaag tgatggagaa aaacaaaagg
      721 tgttggttgc aagagctctt gttcaagaac cagaaataat tatattagat gagcctacaa
      781 ctcacttgga tataaaacat agattggagc tcataaacat actgaaaaaa ttaagtaaag
      841 aaaagtcaat atcagtaata ctttcattgc atgaaataga catagcatta aaaagttgtg
      901 ataaagttgc tcttataaaa aataataaag tcatagctta tggtcagcct gaagatgtag
      961 tagatgagga tattataaat tcgctttatg aattggatga taaaaacttt aatagtcttt
     1021 taggttcagt agaaattagt aataaatcta aaaatgaagt ttttataatt ggtggtgggg
     1081 gtaaagcaac accaatttat agagctttta ctaaaaaagg tataggtctg tattctggaa
     1141 taatacatga aaatgatatt gattatgaaa ttggtagaac tatggggata aagatgttta
     1201 cagaaaatcc atttgagccg ataagtgatg agagtttcga tttggctatt agaaatttaa
     1261 atgattccaa gattataata gatacaggat ttagtgtagg agagacaaat aagaggaata
     1321 tagatattat aaaagaggct ttaaaactgg ataaaaaagt ttattctttt agaaatagag
     1381 atgagagtaa gaagtattat gacagtttgg atagtaagat agaacatata gataaagttt
     1441 ctcaaataat caatagtgca gatataaata atttattata atttatgaaa taaaatgtac
     1501 ttatgtgcta tagaaaaaag agtatagatt ataatttttt tgaaattaca atctatactc
     1561 ttttgttatt ttgtttatat atttataaaa ctttagcatt ttatagataa ccaatagctt
     1621 cctctctagt taaaaaaaag tgaatccctg tagtcgattc tttccatcta ttttcattaa
     1681 aatcttcagc tacaatcgtt tctcctactc tatatataaa attttcatca gcatatgcac
     1741 gagcactttt aaaagattct ttaccatcta tactagtaat tgacagtact tttgccttat
     1801 cacatctaca agcatttgat gtagcagaca ctctttttgc gtcgctgggt attaataatt
     1861 gtaccattct gaaattaaaa cattttttat agcctataaa tgctcctgtt tcaggacaat
     1921 acaatttaaa aaaaggggta ctttcatctg atattactcc atttaaagta gcatgttcta
     1981 aattggaatt ttctaaatta gctcctctaa tatctgagtt agataagtca caaaatctaa
     2041 aatttgcacc acttaagtct gcatttttta atatagaatt ttttaatgaa caatccgaaa
     2101 gataagaatt attaaaaata gagttttcta aatctgcttt ttctaaattt aaattgataa
     2161 ggtcagacca cgaaaagtca atattactta agcagtatcc tcttaagtcc atattttcta
     2221 atcttaattc ttttaaatct gccttttctc cagcattatt gtttttaagc cataaataat
     2281 gtttctcaag tgttttttct aattcttgtt tagaaatagt tttcattaaa atcaccttca
     2341 taaatttatt tttatatatg tagtagcaaa atagtcatat atttaattat ataacaataa
     2401 ataaaaaact tctaaataat atataaaata tcatcttgaa agtacttttt tcaatatatc
     2461 agaatatatt tttaagctat ttagagataa tttaaatttt taaacaggac ataataagct
     2521 tgtatttttt tatatttgag ttgataaaaa tttctttaaa taaaaataat gtcaattata
     2581 aagtaataaa agtttagata taaaaaaatt tgtgtatata ctatatgtag tatataatag
     2641 ataaagcact gactatatgt agtgcttttg tttttgaaaa atacttattg catttctata
     2701 attatagatt aattttggga tttcaactga taagtaccaa aagaaataat aagcaaatta
     2761 agtgaaatta tggcacaaga ttatgttaaa aggacggtaa ataatgaatt ggatagagct
     2821 taacaatcaa gtgataatta aaaatgaaca aggtaagtac caattggaaa aggataagga
     2881 agccttaagt agttacatag aagaatttat acaaccaaag ttgagaaaat ttaataacct
     2941 agaagaaaga ttgaagtatt taatagatga aggttattat ttaaaagaag taataaatca
     3001 gtatagcata gatgttatta aaaatatata tgatatgatt gataaacata agtttgaatt
     3061 tcaatcatat atgagtgcaa ataagtttta tcaaaattat gcattaaaga gcaatgatgg
     3121 aaaggaaata ttagaaacat ataatgataa agtgttaatt gtagctttga cactaggaaa
     3181 tggcgataaa gatttagctt taaacttggc agataaaata gtaaaacaag aatttcaacc
     3241 agctacacct acttttttaa atgctggaag aaagagggct ggagaaatgg tatcatgttt
     3301 tttactatct gtagaagata gtacagaagg tatatcatat gctatatctt catcaaatca
     3361 cttatcaaag attggtggag gagttgcact aaatttatct aaacttagag catctggcga
     3421 atctatcaaa gatatagaag gtgcagctgg aggtgttgtt ggagtagcta aaatgttaga
     3481 acaatcattt agttacttta atcaaatggg agctagacaa ggttctggtg cggtgtacct
     3541 tactgtattc catcctgatt ttgaattgtt gatggatact aaaaagataa atgcagatga
     3601 aaaaataaga cttgctacat tatcattagg agctataata ccagataaat ttatggagtt
     3661 ggcagaaaaa aatgaagtag catatgcatt ttatcctcat actgtgtata agaaatatgg
     3721 agtttctctt gatgagatag aaatggatga gtggtatgat aaattggtta atgatagtga
     3781 tataagaaaa aaagaaataa atcctaggca gatgttgaca aaaatagcac aaatgcaaca
     3841 agaaagtgga tatccatatg tagtgtacat agatacagct aatagagaac atacattaaa
     3901 agatgttgga attattaaaa tgtctaactt atgttgtgag atatttcaat atcaaacacc
     3961 ttcagaaata gaggggtatg gaggaaaaaa tgaatgggga caagatataa gttgtaacct
     4021 aggttcttta aacatagcca atgtaatgga taataaaact atagaaagta cagttgaaac
     4081 agcaataaga gcactgtcat ttgtagcaga tagcacagat ataaaaccag tgccaacggt
     4141 aagcaatagt aacagcaagt ctcattctat aggattagga gctatgaatt tacatggata
     4201 cttagtaaga gaaaatattt tatatacttc agatgatgct atagattttt ctaatgtatt
     4261 ttttgcaatg gttagatact attcaattaa agcttctatg aaaatagcca tagaaagaaa
     4321 tcaaactttt gaaggatttg ataaatcaga atatgttaag ggaagaaata gcaaggtttt
     4381 atcaaaatac tatgaacaat cttatctacc taagtcagaa aaggtaagag cattatttga
     4441 agggatttat ataccaacaa aagaagattg gacaaaatta ttggatgaag taaaagaaaa
     4501 agggatatat aatgcatatt taatggcagt agcaccaact caaagcatta gttatgttca
     4561 aaatgctaca tcaagtataa tgccaattac tgagccagta gaagtaagaa catatggaga
     4621 ctctactaca atatatccaa tgccattttt aacaaatgat aatatgttgt attatcaatc
     4681 agcatacagg atggatatga gaaaggtcat agatttagtg gcaacagttc aaaaccatgt
     4741 tgaccaaggt atatcaacaa cactgtttgt tacagatgaa aagacaacta gagatatagc
     4801 tagacattac atatatgcct ataaaaaagg tttgaaaagt ttatattaca caagaacaaa
     4861 gatgacaaga gatactcatg agtgtctagt gtgttcagta tagggagaga aatatatgac
     4921 atttaattta cataaaatac ataatgcagt taactggaat agagaagaag atggatttac
     4981 acaagctttt tgggaacaaa atgtaaaaca attttggctt cctgaagaaa tatctgtttc
     5041 taaagacata aaggtatgga atgaactaag taataaagaa aaggaattat ataaaaaagt
     5101 attgggtggt ttaacattac tggatacaaa acaggggaat aatggaatac catctatgat
     5161 gagtttaaca gaaaacctac aaagaaaagc tgtcttgtct tttatgggga ctatggaaga
     5221 aatacatgca aaaagttatt cttctatatt tatgactcta ctttctaatt tagaaataga
     5281 tgaattattt gaatggatag aaacagaacc aacattacaa agaaaggctg atttagtttt
     5341 agctcaatac gaaaatacta caaatcaaga gggattatac ctatcaatgg ttacaagtgt
     5401 atttttagag agtttcttgt tttattcagg attcttttat ccattgtatc tatctggtca
     5461 aggaaagatg gtagctagtg gagaaataat atcgcttatt cttagagatg agtctttaca
     5521 tggtaagtat ataggattgc tagcacaaga aatatacgat tcatttgata aaatggataa
     5581 gaagatgtta gaagagaaga tgtattctat actttatagc cttatggaga atgagataga
     5641 gtatacaaat gttatataca gagaaagtgg gcttgaaaaa gaggttgtga actttttaaa
     5701 atacaatgcg aatagagctt tagaaaatct agggtttgag aagctatata ctgtagatgc
     5761 tataaatcca attgtattaa atggtcttag cactgaaacc aaaacacatg atttcttttc
     5821 aactaagggt aatggatacc aaaaaggagt gtatgaggaa ttagaggatg aagattttat
     5881 aatataattt ataattatga cacactgttg tcacttttag cgtgataata tcaatgtata
     5941 aaatatatat ggaggtattt ttatgaaata tgaatggaga aaaagagata aagagttata
     6001 tttacctaaa aagaatcctg taataattga aataccagaa cagaaagtta tcatgttaaa
     6061 agggagtgga gacccaaatt ctaaagaatt tattgaagca ataggagtac tttattcttt
     6121 ggcatatgca ataaaaacca tgcctaaaaa aggaattgta ccagaagggt attttgacta
     6181 tacggttttt cctttagaag gtatatggga taagggagaa aaaagtaaag gcttagatgt
     6241 acttattaaa gaagatttaa tttacactat aatgatacgt cagccagatt ttgttacaca
     6301 agacctgttg gataaagcta ctgatatagt aaagaagaag aaacctcata atcttcttga
     6361 aaaagttagg tttgggtcta ttaatgaagg gctatgtgtt caaatgcttc atataggttc
     6421 atatgataat gaaccagaaa gttttgacat tatgaacgaa ttttgcaaaa aaaataaact
     6481 tataagaaaa tcttacacac atcgtgaaat ttatataagt gatgctagaa aagtatcgcc
     6541 tgaatcattg aagacggttt tacgttttga agtagaaagg gaagtataaa tattttgaat
     6601 aatttataag agttataaaa agtgaagtct gttgagactt cattttttga tgcaatttta
     6661 aaattaaatt tgatgtttca taatatttta aaaactttct acttttatta gtgtgaata
//
LOCUS       DAEPLF010000183         6636 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEPLF010000183 DAEPLF010000000
VERSION     DAEPLF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.183"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            1..357
                     /locus_tag="KR314_002936"
                     /pseudo
     CDS             1..357
                     /locus_tag="KR314_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            475..1017
                     /locus_tag="KR314_002937"
                     /pseudo
     CDS             475..1017
                     /locus_tag="KR314_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            1162..1824
                     /locus_tag="KR314_002938"
     CDS             1162..1824
                     /locus_tag="KR314_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0329305.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVLLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            1824..3005
                     /locus_tag="KR314_002939"
     CDS             1824..3005
                     /locus_tag="KR314_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0329306.1"
                     /translation="MKTPRNINSTVVFIIFCLIITFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(3186..5846)
                     /locus_tag="KR314_002940"
     CDS             complement(3186..5846)
                     /locus_tag="KR314_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329307.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKTVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNTRSRRQEFAMLRSVGLSPE
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5843..6526)
                     /locus_tag="KR314_002941"
     CDS             complement(5843..6526)
                     /locus_tag="KR314_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329308.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
BASE COUNT         2329 a         1019 c          942 g         2346 t
ORIGIN      
        1 tatctactaa attaataaaa gagaatgata ttatttgcat agaagattta caagtaaaga
       61 atatgattaa aaatcataaa ctatctcgct taatttctga tgtatcatgg tcagaattta
      121 ttcgtcaatt agaatacaag gctaattggt atggtagaca aattgtataa agtaggtaaa
      181 ttctttgcaa gttcacaaat atgcaataaa tgtgggtata aaaacgagga agttaagaac
      241 ttaaatataa gagaatggat ttgtcctagt tgtaatgaaa ctcacgatag agatataaat
      301 gcaagtataa atatattaaa agaaggatta agactaataa cgattcaaaa taaataactt
      361 atgtaagaac cgtaggaact acggggatgg cctgtggaga attagtaaga catattttag
      421 tatgcaaaat tctatgaagc aggaaccctg tgactttagt catgggaggt tcagaataca
      481 actatgaatg agttggcaaa agaagttgaa tatactaaac gcaccatata caagtacttt
      541 tcatgtaaag aagatttatt ctttgctgtt gtcttaagag gatataaaag actttgggat
      601 aatgttaaaa tagaaagtgc aaaaggcaaa acagggtttg aaaaaataaa actttcatac
      661 tttgcttttc ataaatttta ctctgtagaa ccttctctat tatgccttat gggaatgatt
      721 ggtattgtaa actctgaaaa atcggatact gatatgctgt ttaaagaaaa gtttttttct
      781 tttaataaat ttatgtttga tgaaatacaa ggaatgtttg aaattggaaa aaatgataga
      841 agtattaggc atgatattga aatacctact ctcatgtatt catcaatatt tacacttaca
      901 ggatttttta atttactatc tgtaacagga aagtcatacc tgaacaactt taatatagat
      961 gaggaaaaat ttattgaaac aactcttgct cttttaatag attcaattaa agcataaacc
     1021 taagtttata aattaaagtt tatatactta aatgataatt ttttctatct ctaacttcta
     1081 tctctaacat aaaaaatatt aaacataaaa ctaataactt agttaatgaa ataactaaat
     1141 tactgaaagg actaaaagta aatggctaaa atacttcttt tagaagatga tgatacaatt
     1201 acatttggaa taaaaacttc ccttgaaaag aaaaatcaca ctgtatgttg ttataatacg
     1261 attgctgaag ctaaaaaaaa ttttgataaa actattgatt taattctgct tgatttaaat
     1321 ctacctgatg gtattggata cgagttttgc caatatgtta aaagtataga agatactcca
     1381 gtgatatttt tgacagtccg tgatgatgaa aaagacatta tacgtggatt agatatgggt
     1441 gcagatgact atgtggttaa accatttctg ctctcagtac ttcaatccag aattaatgca
     1501 gttcttagaa gaactcattc aaatactaac aatactatta catgtggaaa tataagcttg
     1561 aataaagttg aagtcaaagc atatttagat aatgttgaag tacttttgac agcaggagaa
     1621 tataaacttc ttctaagtct tatggaaaat aaaaatagag ctatgacccg tacaaagtta
     1681 ctagaaatgc tctgggatgt agatggtaat ttcgtaaatg ataacactct tactgttaca
     1741 ataaagcgtc ttagagaaaa attaaaaaat ccaccttgta taaaaacatt aagaggcata
     1801 ggatatagaa tggaggacag ctagtgaaaa ctccaagaaa tatcaacagt acagttgttt
     1861 ttataatatt ctgcttaatt ataacttttt tattaagtgt aattctaaca actataactt
     1921 cttttaatga atacaatgta ctgtctaagg ctcttggtgc atcatatgag tacaatccta
     1981 agtctacaga aagtatacta aaatcactga aaaattctag tgataaggat tttaaaacag
     2041 gaaatgaaat tttatcaaaa catggatatt ctaaaagtac attttggagt aaaaactttt
     2101 tttattttct agtgatatcg tttggtataa tttcaacaat ggctacagtt gtatatataa
     2161 tcaaacatag aacaaataag caaaaactga gtagaataaa caatcttact caatacttag
     2221 aagaagtgaa tttaggaaaa gagtctgtac ttttacgatg tgaagatgaa ttttctcacc
     2281 ttgaagatga gatatataaa acagttgggg aacttcgttg ttcaaaagaa tttgctttaa
     2341 aagaaagaca aactctttca gataatcttg cagatattgc tcatcaactt aagactccaa
     2401 ttacttctat gtcacttatg gcacaattac tttcagaaaa ttgtccagat gaagaaatag
     2461 attatattaa cagacttaca aatcaaatct caagacttga gagactagtt tcatctttac
     2521 ttacattatc aaaattggat gcaagtactt taatatttga aagcaaactt atagatgtac
     2581 attctcttct tacttatgca attgagccta tagaaggttc tttaagagaa aaacatcaga
     2641 catttactat cgaggcttct ccttcggtag aatttgaaat cgatattaat tggactttag
     2701 aagctctttt aaatattctt aaaaattgta gtgagcatat agaagatggt ggatatatag
     2761 ttgcttatta tagtcaaaat cctctatatg ttgagattat catagaagat aatggtaaag
     2821 ggtttgctca ggaagaactt ccatatattt ttaatagatt ttatcgtggt aaaaatgcta
     2881 gtaaggacag tattggtata gggcttgctc tatcaaagtc tattatagaa cgtcaaaatg
     2941 gtactctaca tgcagaaaat tgccataatg gtggtgcaag attcataatt aaattttaca
     3001 cataaaatgc tggattatag gtaaaatacc tataatccag ctatatctat attctatgtt
     3061 atctattgtt ttagtaatat acttctattt acatttaagg ttttattaaa caaacaaatt
     3121 aactatccct atacttgtaa gtagtcttaa atcgaaacct ttaaataagt tcaaatagct
     3181 tttgtttata ttgtttcatt ttttatagat tctactatat tttcattctt caactgcttg
     3241 tatcctgtca tatatgatgc tattacaata aaaagtatca atatagtaaa acctattatt
     3301 gtagatatag gtgcaaatgg taaatactct ataaaatata tttcatttat acgtagaaaa
     3361 gcatacacta ttcctatttg cacaggaata ctaagtagta atggtgtaat tcctaagaac
     3421 aaaccttcca aaacaagcat cttttttatg ccttctggag ataatccaac agaacgcaac
     3481 atagcaaact cttgtcgtct acttctagta tttcctgaca ctgtagcaag tacatttgat
     3541 aatccaatca tagccaataa acctgtaata aagcctacta ttaaattcat ggtctttatt
     3601 ccactagcct tttgtttttc actttctaaa acatcctcaa tactataatc tcctgaacca
     3661 tagtctttat tataaatgga ctctaatttt tctcttactc taggaatgtc ctcacgatta
     3721 tttactttaa atactcctat cagcctattt gaacgtaacc tacttacttc atcataattt
     3781 ttagatattt cctgcaccat agacattggc actatttgca ttaatgtata ttttgaacta
     3841 gtatctactg ttttaggaag tgtgcttgta atagctccca ctttcatgtt gtaagtataa
     3901 tcccctttat cttcgtcata tactttttca tttaatttaa tgttatctcc tattgaaaga
     3961 tttaaataat ctatatatac tttatttcta cgtgtactac gattaatatc ttcctcctta
     4021 ttatatacaa ttgaggttgg attttttgta tcataaaata actttgggtc tacagaaatt
     4081 tgcctacaat attctgtaaa actcttatca tcaagcccaa ttatatttga aattatacga
     4141 aacttgccat cacgttctat aggagagtat tttttagatg aaactatact tttaaatcct
     4201 ccatttttcg caagttcttt tgatgcatct ttttcagtca gccaagtgga agcagacatt
     4261 ttttgataat atagtgaact ttttacacct tctgtattat ctaatttttc aatgaaagca
     4321 gactctacag gttcactatt ttccaaataa actgctatat ctctttggtc cttataatta
     4381 tcacttccat aaacagcttt tgctccttct tgatttgcaa ttatatgcaa aaaacatgta
     4441 agtaataaaa aagataatgt taaagatatt gttgcagtac gataagattt tttacgagca
     4501 cgtagtgcgt ttgatgcaag ttctccttct attccaaata tctttcctaa tgaatattta
     4561 tgcgactttt tgattttaac actatctcct tgtcgtattg cttctatagg agatatcttt
     4621 gatacttttc gtgctggtat aagtgcagaa atccagactg ttactattgt aagtaaaact
     4681 gctggcagaa aagcaggaat accataagta aataccactt ctggaacatc agtatgataa
     4741 ttaactgaat taatataaac tataagccta ttacacaata accatcctaa aaataaacct
     4801 attggcaatg gtataattgt aagcaaaaaa ccttcaaaca ctacagacat tttaatctgt
     4861 tttggtgatg caccaacact agataatatc cctaattgag taagtctagc atttgcagat
     4921 agtgaaaacg cgttgtaaat caccattaca aacacagcta ctgtaaaaat tgctattgta
     4981 agatacataa taggagttga aaattttgat atactgctca tagatgcttt ctgttctggg
     5041 gaaaagacta acatttttaa aagataatta ctattatatt taagattgta ttttccatat
     5101 tcattagtct tccaccccaa tgatttagca agttttggaa gttccttgta tgtgtctctt
     5161 atatttttaa atcgcaagta cacagttata ctatcattag gttttatact tgttctatca
     5221 agatatccaa gagctgtata tgcaggtatt actgatgaag tggaaacatc tacctttcct
     5281 actattgtca atgtagtctc tgaaattttt ttaaaacttt cccctttcga tttttgagct
     5341 atgggttcaa gaggctttcc atttaggatt ctatctccta ttggaagagt aattttatcc
     5401 ccaattttta cacttggatt attctcaaaa tattgttttg ataatgcaat ttcattagca
     5461 ttatttggtc gacgaccctc tattatagta tccttttctg gcatagaatt ccaatactct
     5521 gatgtagcat ttctataaat tatataatct cttttctcat cattaatttt gcctacatac
     5581 cattgtcctt ttaccattac atcatcaact gaagaaaaat tttcaattaa tggtaagtca
     5641 cttccataag tatagtcaaa caattcacca tgccagttac cttctttctc aattgaaagt
     5701 cttataaaat ctgtccacat ggtatacaac agtccacaca aagaagacat catggttgta
     5761 gtaagtaata tagctaacat tatagctata ctactacgtt tattgcgtcg tacaaagtct
     5821 aaggtgtatt cttgaagaat attcatttct tcaccacctt ttcagagaca atcactccat
     5881 cttccatagt tacaatacga tttgcctcta gtgctatttt ctcatcatga gtaataagaa
     5941 gtattgtctg attaaatcgt ttatttgata agtttaacaa gtctacaatt tcttctgaat
     6001 tttttctatc aagattacca gttggctcat ctgctaataa aatagctggt ctatagatta
     6061 aacttctagc tatagatacc ctttgttgtt gtccacctga aagttgactt ggaagatggt
     6121 ttaatctatc agataatccc aatatagata caatctcaga gaatctatcc tcatccacct
     6181 ttcttttatc aagtaacatg ggtaataata tattttttct aacatcaagg gtaggtataa
     6241 gattataaaa ttgataaata agacctactt ttctacgtct aaaaactgca agagcttctt
     6301 cctttaaact tgatatttct gtatcttcaa cataaatctt tccagatgta ggtctatcta
     6361 ctcccccaag cagatgtaac aatgtagatt tgccagaacc agatgctcct acaatggata
     6421 caaaatcacc tttttgaact gataaattta cattattaag ggctgtcact ctggtttgat
     6481 ttgaaccata tatttttgtg agattttcac attttaatat ttccatatac aatcatctcc
     6541 tttatggtta aattttagca acctaaaatg acattatagt gacatgaata caaatataaa
     6601 ttactattaa aaatattgtt tagtttttgc acatta
//
LOCUS       DAEPLF010000184         6635 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEPLF010000184 DAEPLF010000000
VERSION     DAEPLF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.184"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(19..426)
                     /locus_tag="KR314_002942"
     CDS             complement(19..426)
                     /locus_tag="KR314_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329309.1"
                     /translation="MEIGKKLKNARVQSGMTQENVAEKINVSRQTISNWENEKSYPDI
                     INVIELSNLYSISLDELLKGDEKMIEHLEESTNVVKSNQKMIWAIIVNIIVVALLIAL
                     NMFIPDNRYFLVGIFCLMVITSSALLYQIIKKF"
     gene            complement(594..977)
                     /locus_tag="KR314_002943"
     CDS             complement(594..977)
                     /locus_tag="KR314_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBH0329310.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFMQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTFGGADVDWYVKAED
                     IVLKNKGNQNAGNPQNHVPQGQTKK"
     gene            complement(993..1319)
                     /locus_tag="KR314_002944"
     CDS             complement(993..1319)
                     /locus_tag="KR314_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBH0329311.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKKFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            complement(1552..4596)
                     /locus_tag="KR314_002945"
     CDS             complement(1552..4596)
                     /locus_tag="KR314_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBH0329312.1"
                     /translation="MKKTLKRLCTGFLALATIVTALPTTPVHAESKQYWTESAERVGI
                     IEKVMNDGSIGSTFNEGMMKVEGKTAYCIDINTDFRNGYKTRADASSRMNANQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFSGARTFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLKKTSSNASITDGNG
                     NYSIAGATYGVFTDKDCTKQLATLTTDENGNTDVAEVKAGTVYIKELSAPAGYKVDKT
                     VYSLKIEAGKTATLNVSDTPKVTDTLIELFKIDMETKKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSYGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTIEETKAPNGYLLEGAYMQAGDKSEQIKGLYVTQITEDDDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDMKPQGSATLKDTAFDIISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTDGAKPIDFTITENGKIVDLTDKAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHIVVTDNNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYVIEELRSESNKGFELIPPFEIVVSRN
                     NLLIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEITIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRAENDYTFVADEEEMKVEIAYTFNASALGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATNKDGNKELEAGKNVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKCVENDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKPEEPEKPETPQIPEKPS
                     RPSDSPKTGDSTNVMAFLVMLLASAGGLAGTYLYKRRKMKKS"
     regulatory      complement(5115..5200)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(5326..6504)
                     /locus_tag="KR314_002946"
     CDS             complement(5326..6504)
                     /locus_tag="KR314_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH0329313.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMFTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIVSIMIKIWLSRFNKFVGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKDMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
BASE COUNT         1847 a         1420 c         1038 g         2330 t
ORIGIN      
        1 cataacaaaa tatcctcctt aaaatttttt gattatctgg taaagtaatg ctgatgaagt
       61 gataaccata agacaaaata ttccaactaa gaaataacgg ttatctggta taaacatatt
      121 taatgctatc agcaatgcta cgacaatgat atttacaata attgcccata tcattttttg
      181 gttgcttttt acaacatttg tactttcttc taaatgttca atcatttttt catcaccctt
      241 tagcaattcg tcaagactta tagaatatag gttgctcaac tctattacat tgataatgtc
      301 tgggtaagat ttttcatttt cccagtttga aattgtctgc cttgatacat tgattttttc
      361 tgcaacattt tcttgtgtca ttccagactg tactcgtgca tttttaagtt tcttacctat
      421 ttccatattc cgattgctcc tttcggtgtc attgtcatat attaaccctc aaatgtctat
      481 ccaatatctt cgacaaatcc tttttttcct tgtcaaaatt attttacata gcttaaatac
      541 ggctcttttg gctcattata ctgtaatttc agagtataat ctatgaccta gatttatttt
      601 ttcgtctgtc cctgcggtac atggttctgt ggattgcctg cgttctgatt gcctttattc
      661 ttcaacacaa tatcctctgc ctttacatac cagtccacat ctgcaccacc aaacgtcttt
      721 cttgataccg tatcggctac gggattgaca agctctacaa ctgcattgta cggaaattcc
      781 cttaacggca cttctggcgg tacggacacg gggataattc caccatgcag actacatttc
      841 aagtcataga tacgcttttt aagctgggta ctcggtgtcc cgtcctcatt ctgcataaac
      901 acatcacgca ccgctgtaaa ttttaattcg ccaaatgttt tctctttgtc aataacaaac
      961 ccgtttgata atctcataaa ttcttcactc ctttactttt cttcaactgc tacaatatca
     1021 tcagcaacta atacataatc ggtatgtccc atatccccga ttgcgtaacc tctgccgtac
     1081 aacttcggat tgacaagttt tactttctgt tcatacttga acttcttttc gccagcctgc
     1141 acgggaattt ccactacaac attttcgcct ttctgtacgt cagaataaag gttatagctt
     1201 cgtctggcta agaccctgcg gttattttta tctctttcaa agataggctc gctttctcct
     1261 gcaaattcaa gagttccaaa agactgtgcc atatctggca cgacatattt catttccata
     1321 atgttttacc tcgtttcttt ctattttgat aaattgattg taatttctta ttctggcggt
     1381 ttgggaagta ccagcaatcc cccagatagt aaagggtaaa atcaatgctt cgcacccttg
     1441 actatccgtg ttcttgcagg ttggtggcag acaagccaga ataagcaaaa gtctgccgtt
     1501 tgacagcttc ggcggagagc gtgatttttc tttcatcata ccgttaccgc cttatgattt
     1561 cttcatttta cggcgtttgt aaagatatgt tccagccagt ccaccagcag acgcaagcaa
     1621 catcacaaga aatgccatta catttgtact gtcgcccgtt ttgggactgt cgctcggtct
     1681 gctcggcttt tctggtatct gtggtgtttc gggtttctct ggttcttctg gcttttcctt
     1741 aaaggtaatc gtctgtccct tatcctcaat atccttatgc tcggtaactt tcttcggctc
     1801 gtctggattg cttaaatcgt acaattcttc aaaagttaca agctgtttac cgtcaagaga
     1861 agtagcgtca aacgtaaaag caacttccac tttcatagtt tcgctgtcag cagtaaacgt
     1921 ataatcattt tccacacatt ttccattgat aagaagttct gcattttctt ctttcaacat
     1981 ctgccagccc acaagtttgt actgtgtacc aacttctaag ccctctaagg ttacggtatc
     2041 aatgattgta acgttttttc cagcttcaag ctctttgttg ccgtccttat tcgtagcagt
     2101 cgtatgaatt ttgatgatac gctctgtgat taaaacggtc tgcccgtcgt cctcaatgtc
     2161 tttgtgttcc gcaactttca cgggttcgtc tggattgctc aaatcataca attcttcaaa
     2221 ggtaacaagg ttcttgccac ctaaagcaga cgcattgaat gtataggcaa tttccacttt
     2281 catttcttcc tcatcagcga caaaggtata atcattttct gcacgtttcc cgtcaatgat
     2341 aagctcggcg ttttcctctt ttaacatctg ccagcctttg agctgatatt ttgtgccttt
     2401 tgtaagtccg tctaatttga cagtatcaac aatcgtaatc tcttttcctg caaggatagt
     2461 cttttcgccg tccttgctgg tcgctgtggt atggatagag atttcttttt cgtattcatc
     2521 agtcagcgtt cctaaatcaa tcaaaagatt atttcttgat accacgattt caaaaggtgg
     2581 gataagttca aagcctttgt tgctttcaga gcgtaattcc tcaatgacgt aagtatcgta
     2641 aggtaacgca cctttgctgt cgtctggttc agaagttcca aaccacacac cgtcctcgct
     2701 ggtcttgcct gcgttggtat tgtgcttatg agaagcccag tcagcagaag tggagaactg
     2761 cccgttattg tcagttacca cgatatgact ttctcctgtc gtcttgcttg tgatcctaaa
     2821 gggaacatca gcaagacgct tgtgtgtgcc tgcaccgatt tttacaccct caatatctcc
     2881 acgcttaatc tgattataga tagaatgggc tttgtcggtt aagtccacga tttttccatt
     2941 ttctgtgatt gtaaaatcaa tcggctttgc accgtcagtt aagtaaccgt cgggagcttc
     3001 actctcaaca atacggaatt ttccataagg taagaggtca gcagaagtag aagcgacacc
     3061 ctcaatatcg gtacgaattg tctttaccac ttcatttttc ttgtatagct tgccctcaac
     3121 caaaacagaa ttatcattta aggaaatgat gtcaaaagca gtatctttca aggttgcact
     3181 tccttgtggc ttcatatcgc ccgtttctaa atctcgcttc tgaattttca caccgccacg
     3241 gataaccttg tctgatacgg aaaactggtt acttccagat aatacggcaa ggtcgtcgtc
     3301 ctcggtaatc tgtgttacat ataagccctt tatctgttcg gacttatcgc cagcctgcat
     3361 atatgcaccc tctaacaagt agccgtttgg agcttttgtt tcttctatgg ttagtgtccc
     3421 aagtggaaga acggcttttc cgtcttgcat atagaagctg tcgccagaca ccttgtatgc
     3481 gtccgctaat tttgtgatgt aatgagttgt cccgtagctg tctgtttcag cgattgtctt
     3541 tgtaacccat gttcgagtag cttcggcagg gagattgtct ttgttataga agccagcata
     3601 atacttccat gtaaattccg cacctgctaa agaagcgttc ccttgcggat tgtctttctt
     3661 tgtttccata tctatcttga aaagctcaat caaagtgtcc gttacttttg gcgtatcaga
     3721 tacgttcaaa gtcgccgtct ttccagcttc aatctttaag gaatatacag ttttatctac
     3781 tttatatcct gctggtgcgg ataattcctt gatatagact gtgcctgctt ttacctctgc
     3841 aacatctgta tttccgtttt catcagtcgt aagggtggca agctgtttcg tgcagtcctt
     3901 atcagtaaag acaccatagg tcgcaccagc gattgagtaa ttcccgttac cgtctgtaat
     3961 gctggcatta ctggaagtct ttttaagttt cgcatttcca acattcagct tcgcccagaa
     4021 ttgccccaat tcctgcccct cgccagagta gatataaccg ccacattcat agcgtccttt
     4081 attttctttg acaaaggttc ttgcaccaga gaaaacttcg tcctgcgttg cctttggaat
     4141 ttcatcataa gaagctcgca cgttatcgca ttgccagcca agatgtacgc tcaatctctg
     4201 ccagactaca cattgttcca acagatagac ttgcttgtag ttcagttcct tgtgagcttc
     4261 gccatactgt ttgacatact ctaaggataa cgccacatct gaaatctggt tggcattcat
     4321 gcgtgagctt gcgtcagctc tggtcttgta gccattccta aaatctgtgt taatgtcgat
     4381 acaataggct gttttgccct caactttcat catgccctca ttgaatgtcg agccgataga
     4441 accgtcattc attacttttt caatgatacc gacacgctct gcactttctg tccagtattg
     4501 cttactttct gcatgaacgg gtgtagtcgg taaagcagta acgatagttg caagagctaa
     4561 gaagcccgta cacaatcgtt tcaatgtctt tttcataaat tttaatactc ctttcatttt
     4621 tggtataaaa atagacgctc atttctgaac gtctataaca tacgagtatc ttgatttcta
     4681 gtgataaatg atttatagtt tgtttgtaat gttttctgat aaattaaagc tttaaattag
     4741 cctttgttgc taacaacctg ctaacactct acggtgtatg agggtacacc agagagtatc
     4801 aacaattaaa cataacaagc acttatgctt taatttacgg cattaaacgc tcccaagagg
     4861 ataatcgaac ttctagctac cacggttcgc agagaactcc taattctgaa gcataagata
     4921 cagttggata tattgtagac accaccatac ttaaaatcat aattaaacta attaataatg
     4981 cctttttatt tcctttcttc atataaattt tccccctcta ggttatctat agtttataat
     5041 cttccattaa tatcgtatat aaattaattt ttactaaaaa attatataaa aaaagctatg
     5101 aaaataaata aatttatatt catagccggt tgcaccactg actcagtata tccataaggt
     5161 gcccaataat agaatatact tcaacatctc tataatattt cataaaaaca attaaaattg
     5221 ctttatttat attaaaaata caattatata cttctattat attatgcctc tatactattg
     5281 tctatctttt ttacattttt taccttttat gagattaatt aataattaaa taaaatcctt
     5341 atctacaaca attacacaat tatattcagg atttatctcc ttaactaatt ttgttatatc
     5401 ttcttttaag tcatcctctg acatgtcttt ggaaaattcg gaaggattaa ctactatatc
     5461 aaatattaaa ttcttcttgc ttccttttcc aactattcta aaatcatgca tagattttac
     5521 aagactattt ttcttaagta tttcttcaag ttcttttttg gctgacataa cttctttatc
     5581 ttcaagacat attgggtcca tgtgaattac tagatatata tctaattttt cagatatttc
     5641 tctctctgca gtatctataa tttcatgtat ttccattata tctatgttgg atggaatttc
     5701 tgcatgtata gatgcaatac atctaccaac tccatagtta tgaataatta aatcatgtgt
     5761 acctattata tatttataag ataacaatag ctctataata gaatttacca tttcttcatc
     5821 cggtgcttct cctaaaagag gatttatagt atcttttaca agagagaaac cagcataaag
     5881 tatcactaat gatactacga taccaacata tccatctata ggaaaattag taaatctagc
     5941 tacaataaat gatataacta cacaacttga agtaaataca tctcctaaag catcaagtga
     6001 aactgctttt aaggctgaag aatcaatttt atttcccaca aatttattaa aacgactcaa
     6061 ccagatttta atcattatag atacaattaa aagtataagt ggtattactt caaattttac
     6121 aggaattgga tttactatcc tttctataga tgactttaca aattgaagtc caaccaacat
     6181 caccataaat gctactatta atgctgataa atactctatt cttccatgac caaaaggatg
     6241 ttctttgtca gctggcttac ttgctaattt aaaacctatc atagttattg ctgaagatgc
     6301 catatcagaa agattattaa atgcatcagc cattatagct atactagatg tgaacattcc
     6361 tatgaatacc tttatcacaa ataaaagtaa attggacaat atacctacta caccagctac
     6421 ataaccatac ttatttctaa catctgtatt ttgaacgttt tcgctatctc tgataaatgt
     6481 tttgactagg attttggaaa acatgaaact tacctccctg ccctttattt tattaacaaa
     6541 ttttataatc tatatcttta tttatgataa cataatttat atataaaatt cttaaaaatc
     6601 actctacttc agtaaaatat tattctatat atcta
//
LOCUS       DAEPLF010000185         6599 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEPLF010000185 DAEPLF010000000
VERSION     DAEPLF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.185"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..981
                     /locus_tag="KR314_002947"
     CDS             <1..981
                     /locus_tag="KR314_002947"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012343997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBH0329314.1"
                     /translation="NLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKKLAK
                     VSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLLHTQ
                     KGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNKGVV
                     AVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRIGHG
                     VKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEVHPFYTFYKDNLHVSINTDNR
                     TVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            1053..1793
                     /locus_tag="KR314_002948"
     CDS             1053..1793
                     /locus_tag="KR314_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBH0329315.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            1993..3144
                     /locus_tag="KR314_002949"
     CDS             1993..3144
                     /locus_tag="KR314_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBH0329316.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLEPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            3169..3924
                     /locus_tag="KR314_002950"
     CDS             3169..3924
                     /locus_tag="KR314_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBH0329317.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKNTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            4116..6503
                     /locus_tag="KR314_002951"
     CDS             4116..6503
                     /locus_tag="KR314_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBH0329318.1"
                     /translation="MKKFTSKKVARFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SDTYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSVKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMDGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVSYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFNIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
BASE COUNT         2657 a          690 c         1239 g         2013 t
ORIGIN      
        1 aatttaccta agatagattt acattgccac ttagatggaa gtgttagagt agaaaccatg
       61 cttgatatag ctattaaaga gaaaatagat ttaccttcaa ataatatgga tgaaataaaa
      121 aaattagcaa aggtttcttt taattgtact tcactagatg aatatttaga aaaatttgat
      181 ttacccttaa aagtaatgca atctaaggaa aatttaaaaa gaattacttt tgaacttttg
      241 gaagatgcaa gtagagaaaa tgtaaaatat attgaaatta gatttgcacc attacttcac
      301 acccaaaaag gtatgagtgt taaaaatata attgagggta taattgaagg aattagagaa
      361 gctgaaagta tttatgatat aaaaggcaat ttaatattag gttgtatgag aacaatgacc
      421 tcaaaagagg cattattagt aattgaagaa gggaaatcat ttgtaaataa aggtgttgta
      481 gcagtagact tgtgtggtcc agagaaagag ggattttgta aggaatataa agatgttttt
      541 aaattagcaa gagaatatgg atataaagtt acaatacatg caggagaagc tgcaagtgga
      601 gaaaatgtat tggatgctat aaatatttta aaggcggata ggattggtca tggggtaaaa
      661 attaaagacc ataaaaaggc gtataattta gttaaagata aaaaaatatt gcttgaatta
      721 tgtccaacta gtaatgtaca aactaagaca gtagattctt atgaggtaca tccattttat
      781 actttttata aagataattt acatgtaagt ataaatactg ataatagaac tgtatcagat
      841 ataaatttaa gttcagaact aaatgttata tttgatactt ttaaactagg attagaagac
      901 tataaaatta tttatagaaa tgcagtagaa gctagttttg cagataaaga aactaaagag
      961 tatttgaata gtttaattta atagataaaa atatttaaac tttaaattaa gctattaaat
     1021 tgacaaaaat acattgtatt aggagaaaaa atgtgaaaga ttatgaagat gttgtttata
     1081 ttaaactagt caaaaaagaa cttgaagaat ggaagaaaaa tatattaaag aaaccttcta
     1141 tatttgacaa agcatcaaag ggtatacaga ataaatttaa tggagtattg ccagataaat
     1201 atcatgaaat aattacttct tctataaaag taatgacaaa aaccgtactg tttggatata
     1261 aatatgctac aaagccacca cttagaaata tgtcattaga agaaagagaa aaattagttt
     1321 tagaaaaagc taaaacttac aagaccacag caatgattga aggggcagga acaggggcag
     1381 gaggaatcct cttaggttta tctgattttc ctttgctttt aggtattaaa gtgaaatttt
     1441 tatatgatgt agcttcaata tatggatatg atgttaaaga ttatagagaa aggatttata
     1501 tattaaacat attacagttg gctttttcta gtcaacagca tataaacaaa acttttaaat
     1561 tgatgaataa ctgggatgaa tatgtaaata gtctaccaga agacatcaat gattttgatt
     1621 ggagaacatt tcaacaagag tatagagatt atttagattt agctaagttt ttacaactaa
     1681 tgcctggaat aggagcattt gtaggatttt atgtgaatgg aaaattaatt gataaattat
     1741 gtgaaactgc tgtgtctgct tatagattga ggattcgaga gcttaagtta taagagaaat
     1801 tataacataa aaaattagtt atacaatata tctatatgta tctaattttg cattgataaa
     1861 aacaattatg gataaaaggt aaagcatgat agaatctaat gtagaggttt gtattttaat
     1921 ggaaaaattt agtttttatt gcttaagata caaattgaat tacgaagaga agtaattggg
     1981 aggaatattt tcatgacaaa tttgccaaaa caatttgata gcttcaatga agctagacaa
     2041 aagggattta taaatgcaaa ggaacttaag gaaagtggga aaaagatggt tggagttttc
     2101 tgtacattta ctccagttga aattccaatg gcagctggag caactgtagt aggtgtatgc
     2161 ggtgttagtg aagagccaat acctgatgca gaaagggtgc ttccaagaaa tctttgtcct
     2221 ttaattaaat caagctatgg acatgcaata acagacacct gtccttattt ctatttttct
     2281 gatttgttga taggagaaac cacttgtgat ggtaagaaaa aaatgtatga agaacttgca
     2341 aaagttaagc caacatatgt aatgcatttg ccaaatactt caaaaggaga atttgcctat
     2401 aaactttgga aagatgaaat gataagatta aaagaagagg ttgagaaatc tttaggtgta
     2461 actataactg aagaagatat aagaactgct ataaaagata aaaatgaaga aagagaactt
     2521 ttaaaagagt tttatgcact tggtaaatta gaaccatctg cacttacagg ccttgagtta
     2581 cataatgttt tatatcaagc aggttttaaa tttgaccgtg cagaacttaa acattcttta
     2641 agaaaagtta tagatgatat gaaagaaaga tatgaaaaag gtgaatgtcc tgttcaaaaa
     2701 gataagccaa gaatattgat aacaggttct cctattggtg gaataagcga aaaaatagtt
     2761 aaaactttag aagatgcagg ggcatcagtc gttgcatatg aactttgtgg agctattaga
     2821 tcaaacgatt tattggttga tgaagaaata gaagatgtat atgatgcttt aacacaaaaa
     2881 tatataaata taggttgttc ttgtatgatg aataatgaca atcgtattga attattagat
     2941 agaataatag atgaatataa tgtagatgct gtaatagatg tagttttaca agcatgtcat
     3001 acatttaata tagaaagtta tagaataaga gaatttgtta caaaagaaaa aaataagcca
     3061 tttatgtctc ttgaaactga ttattcaaaa tcagatactg aacaattaag aactagattt
     3121 gaagcgtttg tagagatgtt gtaggaataa ataaaagtag gaggaactat gtttagtata
     3181 ggagttgact cagggtcagt ggcaacaaag ggtgtgctat ttgatggtga aaaaattatt
     3241 aagaaaataa ttataccaac tggatggagc cctaaaaata catctaaaca ggtctatgag
     3301 ttgctaagta gtgaaattga caaaaaagat ataaaaaagg tggtaggtac aggttatgga
     3361 aggggagtaa tggattttgc tgataagaaa gtaacagaaa taacttgtca tactagaggt
     3421 atttactttt taaacaagaa cataaggact attttagatg taggtggtca agatagtaaa
     3481 gttataaatt tagatagaga tggaaatgta tttaacttta taatgaatga taagtgtgct
     3541 gcaggaactg gtcgattttt agagattact tctaaccttt taggaagcga tattgaaagt
     3601 atagatacat tagctaaagg atatgaacca gttaatatat ctagtatgtg tacagttttt
     3661 gctgagtctg aaattgtaag tcttctagct caaaacattt ctactggaga ggtagcagca
     3721 gggatactta aatctattgc aaataaatcc acctcaatgt tagcaagagg tgaggtaata
     3781 gatgaagttg catttactgg agggcttgca aaaagtaaag aactagtaaa aatgatagaa
     3841 gaaatactgg gtaaaaaaat ttttattgca gaagatactc aaataatagg agctttagga
     3901 gctgctgtaa taggatttag ataaaaacat ataaaaagaa aatgaaaagc tgaatttatc
     3961 ataaataagt tcagtttttt attttatttt gtttatttat aaaggaaaac tttggtataa
     4021 ttgatttaaa tgattgtata tttgtaaata cttgatattt agtcatatat aacattagaa
     4081 ttttaaaaat tagctcaaaa taagggggag aaagcgtgaa aaaatttact tcaaaaaaag
     4141 tagcaaggtt tgttgcactg tttttaatat ctattttagt aatgtcaaca attcctgtgt
     4201 cagctgacaa tagcaataca ttgaatcaga taaaaaaagc agaatacagt gatacttata
     4261 aacaatatct aaatgatgct aaaaatggac gtacagaaaa atataatgga attataccaa
     4321 atccatatca gttagaaggt actcaaattc aaagtaaagg aagaacagct ccaacatctt
     4381 atgaccctag aaaattaggt cttatgacat cagtaaaaaa tcaagaggat ttaggaatat
     4441 gctgggattt tgctgcaatg gcaaccttgg aaagtttctt gaagttaaat aattatggag
     4501 actacgattt atcagaagaa cacttaagat ggtgggcttc agatggtgaa tatggatggt
     4561 ctgtaaatga tatggatggt gctttaaatt atgaagctat gggatattta acatcttggt
     4621 caggtcctaa attagaaaaa gatatcccat acaatggtag agtatctaaa gcacaaggag
     4681 caaaaaaacc aactaatatg aatacagcac ctactgtttt taatgtgaca gatgctgtgt
     4741 gtgtaagtaa tgacataaat tctacaaaaa atgctatatt acagtatgga gcagtaacat
     4801 ctggttatta tgaagatatt aaatatcaaa gtgatgacca aaattcatat tattgtccaa
     4861 caaaaagttt taatacaaat catgctattt caattgtggg ttgggatgat aactattcta
     4921 aagataattt taatgcaaat attagaccat caaaaaatgg tgcttggtta ataaaaaata
     4981 gctggggaga ttataattct gagggtggat atttctggat atcttatgaa gacaagacac
     5041 ttatgtctga tatagataat ttttcaatca aaggtggtaa aaaaccaaat gatgatgaga
     5101 agatgtatca acatgactat gcaagtattg tacctttaat ttcaaaaaag ataacagctg
     5161 caaatgtatt tgatttcaat agaggcgatg aaactctaaa atctgtaatg ttcttaactg
     5221 aaagtatagg agctaagtat gaagtatctt atgctccagt agtaaatggt atacctcaag
     5281 aaaacaatat gaaaaaatta aaagaaggta ctgcccaata ttcaggatac ataacagttc
     5341 caattgattc ttttaatata ccagaaggaa agggtgcaat tgtagtcagt atagagggga
     5401 aaaatggaga gtcaactata ggttctgagt caaatgtacc aggatatgat acatttaaag
     5461 caaaagctaa tttaggtgaa agttatatca tagataatac tggaaaattc tttgatataa
     5521 atagagatag taacttctat ccgtgtaatt ttactataaa agctgttaca gaaaagtctt
     5581 ctggtgagag tatcccgaat gagtctttaa ttggtagtga tagatatgaa actgctatca
     5641 aagttagtca aaatggtttc aattcttcag aaaatgtagt tttagtaaat ggcagttcaa
     5701 tagtagatgc attggcagca actcctttta catctgctat aaattcacca atattattga
     5761 cacaaaaaga agctttaaat tcaaaaacta aagctgagat acagagatta ggtgctaaga
     5821 aagtatacct aataggcgga gaaaattcta taagtaaaga aattgaacaa caattaaaaa
     5881 gcttaaatat atcgatagag agaatatcag gaagtgatag atacaagaca agtttattat
     5941 tagctcaaaa gttaaatggt ataaaaaatg tatctcaaat agcagttgta aatggagtga
     6001 aaggattagc tgatgcaatt agtgtaggtg ctgctgcagc agaaaataac ataccaatca
     6061 tacttgcaaa tgaaaagagc gaattgcaag gagctgatga atttctaaat tcattaaata
     6121 tagagaaatc ttatattatt ggaggaacag catccctatc aaacaatcta gaaagtaagt
     6181 taaaaaatcc aactagatta tctggtagta gtagagacga aactaattca aagataatag
     6241 ataattttta taaaaaagat actttaaaaa atgcatttgt agttaaaaat ggaatcaaga
     6301 atcaaaatga tttaatagat ggattggcag ttggtccttt aggagcaaaa actgggtcac
     6361 cagtaatttt agttggtgat aaattagctg atagccaaaa agaagttctt aaaaataaga
     6421 ctttagaaaa agtaactcaa gttggtggag gagcaaataa aaatgcattt aaagagttag
     6481 taaaattaaa aagtgctaaa tagagtactt aagtattaac atatattagt gtgtattagt
     6541 aacttatctt aataaaagct gaaaataatt actttaaatt attgataaat aagataatc
//
LOCUS       DAEPLF010000186         6556 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEPLF010000186 DAEPLF010000000
VERSION     DAEPLF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.186"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..656)
                     /locus_tag="KR314_002952"
     CDS             complement(<1..656)
                     /locus_tag="KR314_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBH0329319.1"
                     /translation="MKKDTSHIIKRAEHFQVILIGEGLMVGAVAGLIVLLYRILLGQA
                     GTWLSQILKFVEGSTIKIIAWFAVLAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKI
                     KQVWWRVLPTKFIGGFLSLMGGLALGREGPSIQLGAMAGKGISRALDRGKTEEKFLLT
                     CGASAGLSAAFHAPLAGVMFSLEEVHKNFSVSVLISVMTASLTADYISANFLGMESVF
                     "
     gene            complement(843..1352)
                     /locus_tag="KR314_002953"
     CDS             complement(843..1352)
                     /locus_tag="KR314_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBH0329320.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLE"
     gene            complement(1427..1840)
                     /locus_tag="KR314_002954"
     CDS             complement(1427..1840)
                     /locus_tag="KR314_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBH0329321.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNSDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2277..2936
                     /locus_tag="KR314_002955"
     CDS             2277..2936
                     /locus_tag="KR314_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBH0329322.1"
                     /translation="MDEKILELLQEIQGNISNMQGSISGIEGTVNGMQGNINYMQGNI
                     NYMQGTISDMQGTISDMQGSINGIEGTISDMQGSINGIEGTISDMQSNINDIEGTISD
                     MQGSISSMDGTINSMQDSITDMQGEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQ
                     SDVTEIKSDVKINRNTLDTVIKHTSSLVEDTSDIKNDLNNIEIVTSKNWNDIAKLKSV
                     K"
     gene            complement(3543..3722)
                     /locus_tag="KR314_002956"
     CDS             complement(3543..3722)
                     /locus_tag="KR314_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0329323.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(3947..4480)
                     /locus_tag="KR314_002957"
     CDS             complement(3947..4480)
                     /locus_tag="KR314_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBH0329324.1"
                     /translation="MQYISYYHSPIGNILLASDNVGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGEEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     HKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(5298..5870)
                     /locus_tag="KR314_002958"
     CDS             complement(5298..5870)
                     /locus_tag="KR314_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH0329325.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGSCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(5932..6195)
                     /locus_tag="KR314_002959"
     CDS             complement(5932..6195)
                     /locus_tag="KR314_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBH0329326.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDLYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(6195..6416)
                     /locus_tag="KR314_002960"
     CDS             complement(6195..6416)
                     /locus_tag="KR314_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBH0329327.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYSNPKNRVRAKKGGNMY"
BASE COUNT         2295 a         1128 c          812 g         2321 t
ORIGIN      
        1 tggaatacag actccattcc taggaaattg gctgaaatat agtctgctgt caaagatgct
       61 gtcatcacag aaattaaaac agatactgaa aagtttttat ggacttcttc aagtgaaaac
      121 ataacacctg ccagtggtgc atgaaaggct gctgataatc cagcacttgc cccacaggtt
      181 aacaggaatt tttcctctgt cttccctcgg tcaagagctc tagaaatacc tttacctgcc
      241 atagcaccaa gctgaatgga aggaccttcc cttcccaaag caagaccacc cataagtgat
      301 aagaatcctc caataaattt tgtgggcaac actcgccacc atacttgttt tattttccct
      361 gtcatttccc cctcaagctg agggatacca cttccagaaa tcattggctc ccagtccacc
      421 aactttgata caatccacgc aagtactgca agtactgcaa accaagctat tatttttatt
      481 gtagaacctt ctacaaattt taaaatttgg ctaagccaag taccagcctg ccctaaaagt
      541 atcctgtata aaagcacaat caaaccagcc acagccccaa ccataagtcc ttcaccaatc
      601 aaaattacct gaaagtgctc tgcccgtttg ataatatgag atgtatcctt tttcatactg
      661 ttcctccttc atccaataat tctacacaat ctgaacctcc aatgactaaa gtcatgagtg
      721 tgtacagata ttttttaatc aacaaaaata gatttttatt tactaaatat aagaaaataa
      781 ttgttcaatt tctcaataaa tgtaaattta ttcttttagg tatttattct ccacagtcgt
      841 gcttattcca gcaaaatatt tattagttct tgctttctct gtgtatagat tttacgattg
      901 tcctcaaact gtaatgctaa tttttgttta tgactatcat agagttttgc tttgtctgta
      961 aaagtattta gataatctcg aaaattgata taattattcc atgcgacact attccattca
     1021 acaacatgaa tatggtgagt acgagtattt ttctcaaaat ctcccatagc aaaaagtaat
     1081 tgttttggta catcctgacc tcgaaaaacg attccacatt gctctaacct ttcgaggtat
     1141 aatttagctt cctctaaaga agcaacacct acaacaatat caataatagg ctttgcgcta
     1201 atactgggaa tagaagtgct tccaatgtgt tgaatatcaa tggcagtctt atcccatata
     1261 cttttcaata atataatgac atcttttgca cttttatccc atatcatctg atgttccaat
     1321 aatttaacca taccacattt taaacctatc actatatacc acctcaatat aatccacctt
     1381 tatatatata ttacaatttt ttctatcttg ataaacagtg atctagctat ttcacttttt
     1441 cttgacactc gctacattcc ctatcatgaa tagatataat tcctccacat ttagaacaag
     1501 tatatctctc cttctgcttt tgcataaact tctctaaacc gtactgctga acaaatttac
     1561 tattttccat aaggcttgct tgatatctct tgttataact tttttcaaga ttttttatga
     1621 gtttacaagg aaaattgaaa cattcaaaac aataaaacaa ccctttttga cagacacaat
     1681 cctttatttt acacttacgg cagtgttctg gctttcctgt atcgctgttc aaacaaccag
     1741 cacatggctt tttgtgataa caatgcttat agcaaaccat acaattcatt ccacaaggag
     1801 caaacataat cgtatcaata ctctcttttg gcattttcat aacattttac tcctttcaaa
     1861 aatccaattt gtcgaattgt tatatattca tattgtaaca taaaaaatag ctaaaaaatt
     1921 catgcacttt cattgccaat aaattttcaa ggaaatatga aagtttaaaa ctaagaataa
     1981 aaagaaataa aaaggaagct actaatcaaa atattcaaac acaagtaatc aattatgata
     2041 ttaccagcag ttttaaatac aaatataacc acatatgcct caaaaacact tattatagta
     2101 tcttagacac ttagacaata cacaagattc attaataaaa aagttctcaa tatagatata
     2161 tttaaccatg ttttgaatag cagataatta tatgattaaa aattaaatat gacatagcgt
     2221 ttgtggaata taacacctaa attatgttat aataataaaa caggagtgtg atagtgatgg
     2281 atgaaaaaat attagaatta ttacaggaaa tacaaggtaa tattagtaat atgcaaggta
     2341 gtatcagtgg tatcgaaggt actgttaatg gcatgcaggg taatattaac tatatgcagg
     2401 gtaatattaa ctatatgcaa ggtactatta gcgatatgca aggtactatt agcgatatgc
     2461 aaggcagtat caatggtatc gaaggcacta ttagcgatat gcaaggcagt atcaatggta
     2521 tcgaaggcac tattagcgat atgcaaagca atatcaatga tatcgaaggt actattagcg
     2581 atatgcaagg cagtatcagt agtatggatg gcactattaa tagtatgcaa gacagtatta
     2641 ctgatatgca aggtgaaatt aaagaagtca atagtagatt agacaatatg gaacctgata
     2701 ttaaagactt aaaaactgga caagttgaaa ctagtagtag acttgataca attcagagtg
     2761 atgttacaga aattaagtca gatgtaaaaa ttaacagaaa tacattagat acagtgatta
     2821 aacatacctc ttcattagta gaagatacat cagatattaa aaatgattta aacaatatag
     2881 agatagtcac atctaagaat tggaatgata ttgctaaatt aaaatcagtt aaataatata
     2941 ccatagtaaa aaatacctga aaatttcaca tacttcttta aagttatacc aaattttata
     3001 cagaaatact gattatccga caacacactt tacttataaa taatttatta aaaaatactc
     3061 attttatcct aaaattaaca cgactaaatg agtcttgata taataataaa aaataaggga
     3121 gattgaacac taaaaagttg tgtatctccc ttatattcta taatatagtt atataatttt
     3181 tcagcctaaa ataaaagtcc atctttgatt aaaacatatc cccttatcac ttataaaagg
     3241 ataaaatctt gttttcattt ttccatttat atcttcttgc ttaagagtaa taattttttc
     3301 ttttgatact ttaagtgttt ggttaaattt aaccaatcga ctaagttttg ttcaacatct
     3361 atttttcata ttttccaata cttaatctat tttttataaa taattctttt atttctaact
     3421 cacttacatt ttttacataa aacgaagcat ataaatttca accttatctt gtatggttct
     3481 taatacttaa tgagtagctt aagtatcaat cattataaat tttcccctta tatttaacac
     3541 tcttaccatt tccaaaatat acaaaatata ttttgaacat ttgtacaatg atgaaataca
     3601 atacatgaaa atacaatatc ataactgtta tctaaaaata gaagttcttc aacataacct
     3661 ttcacaaaga acatattatc taagtatttt ttgtctgctc cttgttttcc tgtttgtagc
     3721 atataaatta ctgcatctaa acatataact actcacacga aaaaagaaaa taaacttcca
     3781 aaatacaagc tctaaaacta cctctaaagt tatataatgt ttactaggaa taaggttgta
     3841 taacatatag tttaaagaat agttttttaa aattgcaagt tattgctttc aaaattagat
     3901 gtttatatat caaataaatg attaagaatt tctaaattta atggctttat aatgcactcc
     3961 ctttctttgg gataaaataa tgttgcacag atactttttc taaagataac aattttattt
     4021 ttctatcaat tccaccagca taccctgtca aactcccatt tgttcctaca acacgatgac
     4081 atggaacaat aatagagatt ccattatgcc ctactgcacc accgattgct tgtgctgaca
     4141 ttttagatat tcctttttgt tgagcaactt tatgtgcaat ttctccataa gtgattgttt
     4201 taccatatgg tatttgttgt aaaattttcc acactgtagt ctggaaagga gtacctacca
     4261 tatggagtga tggtgtaaaa ttgggctctt ctcccgaaaa ataaatatct agccatttct
     4321 ttgtctgctc gaaaattggc aattcttttt cttgatgttc ttggtcaaga ttataagcat
     4381 aatacttttg attctcaaac caaagaccag ttaacccaac attatctgat gctaaaagaa
     4441 tatttccaat tggactatgg taatagctaa tatattgcat acaattcctc cttatattct
     4501 gaaaacttca ctcaaatcat tagacacttc ttttctatct gccattatgt aaaaacatac
     4561 taatataatt tccttctgac tacactttcg ttataatatc tatcctcata tcatattagt
     4621 tttatattta cctgaatctg ttcatccttt ttttcctttc cctttgatgg atataaaatt
     4681 catacattta agcttcctac tgtattaact tttatagaag tgtatattat aattttatca
     4741 ctaaatagac aaaaaaatat cgatataaca tcaagaaata ttttactgag tttcgcttat
     4801 taaaagcaac tgaatttcta aagggtacag atgagctaat aagtgatatt gatataaaag
     4861 ttgtttttca ttaagtaatc tattttagta aatgttttaa agaaaaaacg ggatattctc
     4921 caaaagtgta cagaaatata aaaaaagaga tatgattttt tatttctttt gattatccaa
     4981 agagtaaata aaaaatattg taaaaaactt actaacaacg gcaatgcttt gaactatggt
     5041 aatactttga acttttgatt ggactttttt tgctaaaata atatatttaa atagcaaagt
     5101 aatctgttta atcaataaaa aataagagat aaaagaaatc aactaaaacc tttacctctt
     5161 attttgatag tttatcagag ctaatattta cactcttttt atttttgata cttcattaaa
     5221 aatgatgctt attctctttt catttttaat acttcattaa aattgatgct tactctctat
     5281 tcacttttaa tacttaatta gaactgatgc ttacactctg taaactcata aacatccatt
     5341 aatgcttcac caaatctttg atagtgaact acttccctct cacccaaaaa tctcaatata
     5401 tctttcaaat cagggtcatc agttaaattt attaattgat agtacgtagc caatgctttt
     5461 tgttcagcag ccatatcctc atgtaaatca gttaccgaat cgccaaagac attaatataa
     5521 cttgtagtaa agtttactcc attagaatct actggttgaa gaccatgtcc aaaattagca
     5581 taattactac caagtccagc agccttaagc tcttccggag tacaactgcc tattaattga
     5641 tataacactg aagaaattat ctcaacatgg gccatttcct ctgtacctat atcagttaaa
     5701 agtgcgcgag attttccagt tggcatagta tatctttgtt gaagatatct agttgcagct
     5761 gccaactcac cattaggtcc tcccaactga gtcatgatat attttgccat tctagggtct
     5821 gcttgtctta tgttaactgg atgttccagt gttttttgat aaatccacat attatcttac
     5881 tcctccttaa tatatattta ataataattc taattttaaa acttaataat tttatttata
     5941 aaatgatttg tcccatggcc aaggttcaga tatccatgac caaggatatt cacttgattc
     6001 atatccaaaa ttagttaatg ctccatattt agattcataa tcacacacag cttgtgtaaa
     6061 tttatcgcag tatctattgt ataaatcaag agctttctta tcccttggat tgttatctaa
     6121 atataaattt aattctatac aggcaaattg atattctgaa attctctcta aaagttctct
     6181 tctatttaat gacattagta catgttaccc ccttttttag ctctaactct atttttagga
     6241 tttgaatata gccaatcatt atagttattt gactctatta aatctaattc aggaaataaa
     6301 gtacctcttt ttaatgctgt ttttggattg tatgtgtcat cataaatttg acgattgatg
     6361 tatggtctag caaattcaca acaatagtct tgtttatcct cttttaaata tcccattaat
     6421 tatcctcctt aatttttaga attttaaaat agcattattt ttaaaaattg tatacttgtt
     6481 ctatattata gtcagtagtt ttaataggtc tttaataaga ttaaataatt aaataagtat
     6541 ctttttttgt tcttat
//
LOCUS       DAEPLF010000187         6554 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEPLF010000187 DAEPLF010000000
VERSION     DAEPLF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.187"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(75..2213)
                     /gene="vanT"
                     /locus_tag="KR314_002961"
     CDS             complement(75..2213)
                     /gene="vanT"
                     /locus_tag="KR314_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBH0329328.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(2231..3037)
                     /locus_tag="KR314_002962"
     CDS             complement(2231..3037)
                     /locus_tag="KR314_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0329329.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(3034..4134)
                     /gene="vanG"
                     /locus_tag="KR314_002963"
     CDS             complement(3034..4134)
                     /gene="vanG"
                     /locus_tag="KR314_002963"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBH0329330.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(4395..5477)
                     /locus_tag="KR314_002964"
     CDS             complement(4395..5477)
                     /locus_tag="KR314_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0329331.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(5527..6228)
                     /gene="vanR"
                     /locus_tag="KR314_002965"
     CDS             complement(5527..6228)
                     /gene="vanR"
                     /locus_tag="KR314_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBH0329332.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKAQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
BASE COUNT         2272 a         1151 c          738 g         2393 t
ORIGIN      
        1 cataatatta ctaaaaatgt ctagtcagag taaaataaat tacacagtac aacaaatatt
       61 actatttaat tgagttactt ctttataaca tttaaccttc ttcctactct gctcaataat
      121 tcattcgata tactacatga gttatttgca acttcagaag cagataattc agataaatta
      181 tctcttccta ttattattgc cgtattacca acttctacat taggtatatc tgtaatatca
      241 acagcaagtt ggtccatgca aattcgaccc actattgctg ctctacaacc atttattatt
      301 acgtaacttt ttccacagga taaatttcta ggtataccat cggcatagcc aatagataat
      361 atagcaatta cgctatctct gcttgcaata aagactcttc catatccaaa actatcatct
      421 ttttgtattt ttctaataag aattacttgt gattttaagg ataaaacagg tcgtaaatca
      481 agttgcaatt tagtcgtatc atttggagaa cttaatatcc cgtataatgc aattccaatt
      541 cttacataat tacacttaag attgggataa ttaaaaaaac cataactact ttgaatatgt
      601 actttaggaa tttttatacc tttttttaac agaaaatcaa ttaacttata aaaacattca
      661 atttgtttat ttgtaaagtc tacatcttca tctttcagac tatctgaaac acacaagtgt
      721 gtaaatatgc cacatatatc taagtatttt aatttaaatg cctttaaaat tgacctcaca
      781 tcattaacat caaaacccag tctgtgcata cctgtatcaa ttttaatgtg tgattttatg
      841 ttgtatttct gtttgtttaa agagatagca tgattaaggt caataacact ctgtgtaaga
      901 ttgtatttat gaagttcctt tgcccttatt gggttagtat atccaagaat caatatttct
      961 cctagtattc catatcgacg taaatcaatc ccctcatcta ttgttgctac tgcaaatgct
     1021 tttacaccta tttggtttgc acaagtcgct acttcaaaag cactatgacc ataggcatta
     1081 gcttttacaa ctgccatcaa ttcacaatct tctggcatag ctttttgtat catctttaca
     1141 ttatgttcca aattatttaa atcaatttca atccaagctc tattggtatt taccttatgt
     1201 tctcctttca agtctttttt tagaggttta agaatcaata taataattat agaagctata
     1261 aaagatgcta tacttaccac tatataatga ataaggctat tgtcaaccaa gacagtttgt
     1321 attccgaata ttttagaaaa catacgaata acaacaatca tcataggatg tataatataa
     1381 ataattaatg atgatgttcg caataaccta gctcgagaac cttttaaaaa tgttaaggct
     1441 gtaaataaga aatacataca tggcaataac ataatataca tgctatcatg acgctgtacc
     1501 cctagtttat gtaaaaacat tccctcacat aacataagta agaatgatat aagaaatcca
     1561 atcaagctat ttttagttga catataatta gatttattag atattattcc accaagtatg
     1621 aaaaatacag gtgcaaaaaa tataccattt cgtgtataat ctgatacttc aaaaatatac
     1681 ccatataaat tctttaatag tggtattttt tctaaaaaac cataataact atcaccaaac
     1741 ataccaatta cataaataac aatagtgatt actaatgagt actttaaacc tagttttttt
     1801 acaagaaagt atgcaatact tgcaccaata attgatgcag gtagatacca taaatgatat
     1861 aaagtcccat caaatacaat gtccttaata atattgggca ctaaattatt catggaaaaa
     1921 tatccagtgt aaatatttaa gggaatataa attacaatag ctatcccgta aataaataat
     1981 acatttttta gaaaagattt cagtttatta caattataat tatatgttga aattagaaaa
     2041 aagcctgata gcataaaaaa gaatggcaca gcaatacgag caattatact agttaacaca
     2101 aagtcagcca ttttactata tgataataat ggagatgtat gaattgcaat tataagtaat
     2161 gcagctataa tcctaaaata atcaagccct gcataagatt tattcctaat cattcttgtt
     2221 cctccatttc ttagtccttt ctatttctcc aaaatgtcac aataaaacca tccttattgt
     2281 tacccgatat ttgatataca cattgttcag gtatagagat tctcacttct tctttagtat
     2341 gaataggcac ataaaagatt tctataattt tttctttatc ttctattttt aaacaatttg
     2401 tatacgaaaa tctcttaata tattctatat attcttcaag ggcgagattc attttattga
     2461 taatctcaga atgtggatat ccgacataac gaaaatgcca tggttcttga gatattcctg
     2521 ttatttcttc ttttttacac tgataacgct ctataaaacc ataatgtagt gctgccttac
     2581 ggaactcatt acaaatacca ttataaggaa attcagggca gataaaatct ataacctctt
     2641 tcttcaaccc taaatcaata gcaagccctg tctgatgttc actgtgattt ggaagtgcta
     2701 caaacttttt agtaaagtct tttccattgt cacgcagaga gcttgaatat atatcttgtt
     2761 gttcctctgc agtacgatat ccacttacag gtactatttc atttattcca catatacttt
     2821 ttaaaatatt ttgtaaaata tttgtagctt cacgtttcat aagaatactt ggaaaccttg
     2881 tatctacagg aattaaatct atatcttcag atgttttaac tggtaaaaaa gcatttacta
     2941 gaattagatt cccatcataa actttatctt tagtcaataa gatagtattt gtcattttat
     3001 cttctttatt tttatctaat aaaatattat tcatcatttt atgtacacct caattatctt
     3061 atccaatatt tcttcaaaag acagtccaat acctttcatc atattcggat aacgactatg
     3121 agaagtaaag ccaggaattg tattgacttc attaaaaata atttcaccag atggagttaa
     3181 aaacatatct actctagcaa atccagaaca tccaagagtt ctataaataa tttttgctgt
     3241 ctcttgtatt cttttctcac aatccaaatc aattctagct ggcatatgaa tttttgaact
     3301 tttcaatgta tatttttctg tataatcaaa aaatcctttt gacaattcta tttcatctac
     3361 tcttccaaca agtaagtcat cgtttcctaa aactgcacac cctacctcaa aaccatcaat
     3421 attttcctca attattactt cattatcatg ttcaaaagcc aagtctactg ctacttgtaa
     3481 ctcttctttt ttataaattt tactaatacc aaatgatgaa cctgcacgca caggttttat
     3541 aaataatgga taagttaagt cttttgacag ttcagccaca ttaaatttat ctgagtattt
     3601 tagtaatatt gattttggta ctttaatacc ttctgaatga acaagtttat gtgatttgtc
     3661 tttgtccata cataatgctg aagaaagagt accacatcca ataacctgta tacctgccaa
     3721 ttcaaataac ccctgcactg ttccatcttc tccatttttt ccatgcaata caggaaatac
     3781 aaaatcaagt ttagtgtatt taatatcatt ttcacatgat tcaatagcac tatttggaat
     3841 atgattctga gaaacagcat caggaattaa aaattcaata ataccatttt tgacacggtt
     3901 ctgcgaaaca ataactggaa ttatatcagt atccctttca aaccaagtgt tattctggat
     3961 attattataa tctccaatat aatgatacca attaccctcc cttgtaattc caattggtat
     4021 aatatcatat ttttccgtat tcaaattctc aagtactgca aaagctgatt gtaatgatac
     4081 atcatattct gttgagtttc caccaaatat aactgcaatt tttttattat tcatctttga
     4141 ttctcctttg ttgatttatt tatttttata caatctacag aaacatgtta aaatactttt
     4201 tctatttcaa atccaatcaa ccttcactta caattgcatc tgttccacat aatatatttt
     4261 cacatttttc tattgatata attactgtat caaaataaaa gcactcttgt tttgatacag
     4321 taattatatc aataacaaga gtgctcttta tttatttatc ctaaagaatt tcctattttt
     4381 ttatacttaa attcttacaa atttcctaca atcggaataa taaccttaaa ggtaattata
     4441 ttgttctcgc tatgagctgt tattgtcccg ttatgaagct ccactatttc tttagctata
     4501 gcaagtccca acccagctct accactgttt gaattacgag atgtatccaa cctgtaaaat
     4561 tgttcaaaaa tacgctcaag tttttcctct ggaatcgtat tgctatgatt tataaattta
     4621 agctttatat aatcatcctc ctgtacagct gttatttcaa tagtagtatc ttcaaaacta
     4681 taataaacag catttcgtaa taagttatca aatacacgtt gcattttatt aacatcacac
     4741 cttatcatca tatttggtgt aacattcagt gtatatttta aattcttact catcaaagta
     4801 ggtttaaact catatgttag ttgttcaagc atacgagtta agcttactgt actgtattca
     4861 agtgatatat ttgataaatt aaaccttgta atttcaaaga attcattaat taaatcttca
     4921 agacgctctg ccttatccaa cgaaatagat aaatattttt ttcgcagttc ttcagaaatc
     4981 tgactttcat ctcgaagtag tgtcagatac cctataactg atgtaagtgg tgtttttaaa
     5041 tcatgtgcaa gataaacaat aagttcattc ttacgttgtt ctgccatttg tgtctcaaat
     5101 ttacgttgtt caagtatatg tttaattgag tttattttct tttctattgc caataactct
     5161 ggagataaag ccacttctcc aacatcttct ttgataagag agtcaatacc ttgatttatt
     5221 tcactaaaat attttgtaaa ccaatttaaa taaacacgga atattataaa aaacacaatc
     5281 accatagcta gtatcatgaa gagctccatg ctattacgga aaatctgctg atataaattc
     5341 aatgcagctt tatattccat acgaaaagca ttttcaaaga atgatactat ccagatagaa
     5401 aacttaccat acaatataaa agaatataaa atccatatga aaataatggc tataaatgcc
     5461 attaaagctg ttctcataaa aattttattt ctcacttgag taaagtcagg attgacttta
     5521 atatttttac ttttcaattg tataacctac tccccataca gtttttataa atttcgggtt
     5581 tttagaaggt tcatgaattt tttcacgtaa tcgccctata tgagccataa ctgtgttatt
     5641 attatcaaga tatttttctc cccatacagc ttcaaacaat tcttcagaag gaaccacttt
     5701 tccttgatgt tcacataaat accaaagaat agaaaattca atcggtgtta atgctacttc
     5761 tttcccaaat aaactacatt tatgtgtttc tttattgata atcaatcctt taatatcata
     5821 ctcattaaca attatcgact gttgttcata agaattatta tatcgcatat atcttcttaa
     5881 ttgtgccttt actcttgcaa caacttccaa agggttaaat ggctttgtta tataatcatc
     5941 agcacctatg gttaaaccca ttatcttgtc tatatcttca attttagctg ttaacataat
     6001 gataggataa tagtattttt ctcgaatttt ctggcaaata tgaaatccat caatatcagg
     6061 aagcataata tctaaaatag ccatatccaa atctttagat tcaatacatt ctagagcttc
     6121 cttacctgta taaaacttgt atactgtaaa accttcattt tttaaatata cttcaattaa
     6181 atctgctatt tctttttcat catccacaat caaaatattt tcgttcatag attatttttc
     6241 actctcctta ttatcgaggt catcatttat aatattggta aatttacata tcaaataatt
     6301 acaagcatat ttgtaactaa tttttataag aaaagaacta ctacatcaga atgtgttgat
     6361 gctactattg gaacacaaaa actgagatta attgttatta taaaaactcc cctaacacgt
     6421 ccttattatc gcataacagg tattaacttt caataaaaat atctatcagt caaacacaat
     6481 tcaatttatt ataccagaga caatccatat aaaaaacaat taaacctatg tatgtaacca
     6541 cagacattac taaa
//
LOCUS       DAEPLF010000188         6544 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEPLF010000188 DAEPLF010000000
VERSION     DAEPLF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.188"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            187..1440
                     /locus_tag="KR314_002966"
     CDS             187..1440
                     /locus_tag="KR314_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBH0329333.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIGLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            1537..1761
                     /locus_tag="KR314_002967"
     CDS             1537..1761
                     /locus_tag="KR314_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0329334.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(1977..3283)
                     /gene="grdB"
                     /locus_tag="KR314_002968"
                     /pseudo
     CDS             complement(1977..3283)
                     /gene="grdB"
                     /locus_tag="KR314_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
     gene            complement(3369..4655)
                     /locus_tag="KR314_002969"
     CDS             complement(3369..4655)
                     /locus_tag="KR314_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBH0329335.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            complement(4934..6256)
                     /locus_tag="KR314_002970"
     CDS             complement(4934..6256)
                     /locus_tag="KR314_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBH0329336.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            complement(6256..>6544)
                     /locus_tag="KR314_002971"
     CDS             complement(6256..>6544)
                     /locus_tag="KR314_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH0329337.1"
                     /translation="DAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALKDIFNPFY
                     TTKPIGEGTGLGLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
BASE COUNT         2070 a         1138 c          715 g         2621 t
ORIGIN      
        1 ctacggtttt tcattctaaa aaagtcacat ctcattaaaa tattttaatt taatttatga
       61 ctatctaatt atatatactg attcttttta atatacattt attaatatac atttgataat
      121 aaaatttcaa tacataagaa tttaagttat ataattaaaa ctttgaatta caaaggagga
      181 cttattttga ttaccttaag ttttgatata gtgcaaacct tgacactatc tatattcttc
      241 tttttaattg gtaatttatt gaagaataaa gttaactttt taaataattt ctgtatacct
      301 gcaccagtaa taggaggtct attattttgt tttctaaatt tattttttaa atattttaat
      361 atagcagata tatctataag tggaaattta atgccaaatt ttataacctt cttttttaca
      421 actataggac ttgaaataag cataaatctt ataaagaaag gtggtagtgt attatttaga
      481 tattggattt tgtgtggagt attggcattt tgtcaaaata tattagctat tacaatatca
      541 aagattataa aacttgaacc tcttttaggg cttatgtgtg gaaatgtatc tatggaaggt
      601 ggtcatggtt attctgctgc atttggactt acaatagaaa atctcggtat agaaggagca
      661 gttggggttg gattatctgc tgcaacaata ggacttatta tgggtggtat attgggatgt
      721 cctgtagcta aatttctaat aaataaatat aaattaaaac catcatcaaa tataggcttg
      781 tcgattccta gatataatag aaatctaaag agatttggaa gtaaattttt tagaaataag
      841 aataggataa aaaataataa cttctccgaa acaataacac catctgtatt tttggaacag
      901 gttcttctca tatttatttg tataaataca ggagaaatta taagtagatg cttctatata
      961 acgtttaata tactattacc ctctgttgta acttgtatgt tttcagctgt tatttttaga
     1021 aatttaaatg ataagataaa tatattagaa ttaaatttta aacttataga ttttttaaaa
     1081 gaactttcat tgggaatttt tttaacttta tcacttatga atatagattt atttgaatta
     1141 tctacattat tacctcctat tctcttaata gtaacttttc aagtaatctt tataatccta
     1201 ttttcaatat ttatttgctt tagagtactt ggaaaagatt ttgattctgc catcataata
     1261 agtggtttga ttggacatgg aataggagct actccgaacg ctttggcaaa tatgagttct
     1321 ttgacacaaa aatatggtga ttctccaaaa gcctttttgg ttgtaccatt agtaagtggt
     1381 tttttgctag atgctattag tatcccatgt atactatttt ttataaacat actaacttag
     1441 tatgtttatt ttttgttata tattgacaat ttactctttt tgttatatat tataaatata
     1501 aagtttgata atgattttca ttaggaggtg ttaataatga ctgtttacaa cttaaaactt
     1561 ggacaaaagg gaattataga caatatagct ggaaatgaaa aacttatgaa gagacttcta
     1621 gccttaggac taatagatgg cactgaagtt gaagtgaaaa agatagctcc tcttaatgac
     1681 cctatagtga ttagatttag agggtttgac ttagctatta gaaaatctga tgcaaaaaat
     1741 ataaatttaa aaaataatta atatataata aaaagagatt ttaaaatctc tttttttgtt
     1801 tatgtataat cttctattta tttttttcat tctttatact ttaattggct gttttactat
     1861 aaatattttt attacttaat tgctttcttc attccatcca tagcaaaaag atatcataaa
     1921 ataattttca aaaactattt tatgatatct ttttttattt ctatattata tttttattat
     1981 tcaaatactg tttgttcttc tatatcctct gttagtgcat ttaaagcttt taaaactata
     2041 ttctttctaa tttttttact ttcctcttct cctaacatag gatttccaag tggatatggt
     2101 atccctactg ctggaacaat tctattagct cctattgtaa gtgatattgg tactactgta
     2161 catatatgaa caactgctat accagctctc tcaatctctt ttaccatcgt tgctccacaa
     2221 cgagtacaag tacctcatgt agaagttaaa attacagcat ctacatcatc ttctaacaat
     2281 ttcttagaaa actcttctcc aaatctctta gaattgccaa cactcgttcc tgttcctgta
     2341 gtagttataa aataatctac taattctcca attacacctt ctttttcaag ctctctaata
     2401 catctaatgg tacaactaaa tttggatttt caaggacata tgctctgtca taccccccat
     2461 gtatagttgt aaattcttta tttgacatag aatccatatc tgttaaatcg taaattccat
     2521 atttagtagc acttgaagat tcaattttgt ctggattatc aattggaact atccctccag
     2581 aagttactag ggcaagtttt atcttagata aatctttaac aggtttcata ggtgtaactc
     2641 tatcaaattc aggcattgga tactcagttt caaagctttc ccctttcatc tttttaacca
     2701 acatatcaat tgctctttca gaccctctaa gttcatggaa gaaatttttt cttattcctc
     2761 tcattatata tccttcctct tcaggaggtc ctatttcctc acctctaacc aatttaagac
     2821 ctagtttcgt cattattgga actacctttc taagacttgc tgctgaattt cctgtagaca
     2881 ctatatgcaa atctaattta aacatatcta cacctggatt ttctttatac attcctgtaa
     2941 ctacaggaat atttaactct tcttcaactg ccttacaaat agtcccacaa gctactccat
     3001 atctacctgc attaaatgct gggcctgcaa taaataaatc ggggctttct tcttttatca
     3061 tttttaaaag ttcttgagtt gcactctcaa tattttcacc aaaatacgta tctccacaaa
     3121 ttacagttga gattatctct gcatcatcat taaattgact ctgaaatgct attccaggtc
     3181 ctactacacc agcccttttt tctggcggaa tatctgcttt ttcctctcca cctataccag
     3241 caaagaaatt gtttatataa tgaaccactc tgattttact catctcttaa tcctccaaat
     3301 cactttatag ttattacatc aattagacca acgattacat caacagaact ttatattcaa
     3361 atacttattt aataatatct tgctgtcaaa tgattatagc catttgctat agttgatgct
     3421 attataattt gtagctctgc atccatgctt ccgtcttcat ttaagcatcc tttataccca
     3481 cctatcatca tttcaacata atctaaagtt cctataattt tttccattgc tgggaaatga
     3541 accactagat ttccttgacc acaagaaaca actgcatctg cttccttagt agcgtctgct
     3601 atagattgac tcttgccatc ttttccagga aattcatccg ttatcaaaac tacatttgct
     3661 ccaacttcac tacattttct acaattcatc attaggtcag tatctggatt cccataacct
     3721 tcttctgtca ctagaactcc atcaactcct aaaaattcta ttaatttagc taccatatca
     3781 gagcttctct ctttatcgac taagaatacg ttctcattag tcaaaataac tcctataaag
     3841 tttaaatctt ttccatgtct tttatataag tcatgtataa ctggattatt taaatgatgg
     3901 aatgttgtaa ccttatcaca tggtgctaca cagtttccag atattatagc tccatccatt
     3961 atttcagttg gatacataaa tgtaggcact atctgtttag catccacccc atagtaataa
     4021 gtgtcatgta aaagtccttg tgattgtaac atgtgaacat atgctactgt tggcaggtct
     4081 ggatattctt ttacttgttt taataatggc tttgtctcat atacaatgac ttcatctggc
     4141 tcaacttctc ttcctgcctc acctacatat gcacctattt ttaatcctgc aattctagct
     4201 gcttcttcat aatcatgagt atctatacca tcctttgctt ttataacaat acatatatta
     4261 tttgtttttg aaaaaggtgt atatttagca gtaggaccac tcatatcaat tacgccttct
     4321 tgaaatccaa ctatatttcc acaagttaca acacaagctc ctaaaagtac atgggttctt
     4381 ccacttccta cagtcttcac tttatttata attccaggaa atattttgct ttcatctcca
     4441 attttaactc taggctctat tacatcttta actggagcta ttcttattga ttcgccaggt
     4501 cttgcaagtt caacatttac acttacaatc ctttcatctt ctaatactat cttttctatt
     4561 tcttcgctac taatatatag tactccatct tttacttctg tcttttctcc aaattcaaca
     4621 ttttttataa atatattccc tagctctagc ttcatatgaa aatccccctt aatatattta
     4681 atcgcaatta aaaatattta ttacgtttat taatatataa agcattttct atgccaatta
     4741 tttaaaatat atattctatg tattaatgtc atagaaattt tcatttttaa tttacaccag
     4801 tattgaattt accaataatt attgtaaata ttaattttta aaattataat aaaaaaagat
     4861 aaacagattt tttctcttta tcttttaata cagtttgaga aatttttttc tcacttaatg
     4921 agaagttttt ttctcactta atattatact ctgatatttt attggttaac tgtcttctac
     4981 taatctccaa ttttttagct gtatttgaaa tattatttcc acacaaagat aaaacttttt
     5041 caatatactc acattcaaac tcttttctta tttctcttaa aggtctaata cttattttat
     5101 catctatgta tacattattt gatattaaat ttaaattgtc ttttgataaa ttgccttctt
     5161 cacttaaaac tacaagtcta tttattatat tttttaattc tcttatattt cctggataat
     5221 tgtaatttaa taaaaaatcc tttacttcct tatcaatcga gtgtatcttt ttatcatgtt
     5281 caatttggta cttatttaag aaaaattcta taagagtagt taagtcttct cttctctttc
     5341 ttagtggtgg tatagttatt gttattgtac ttattctata aaaaaaatct tctcttatat
     5401 ttccatttga tatttccact tttggttctt tattcatagc acaaattaac ctaaagtcaa
     5461 ctttaataga tttattactt cctatcctct ctatagattt gtcctcaagt gtcctcaaaa
     5521 gttttacctg aacatttaga ggtatatctc ctatttcatc caaaaataga gttcctttat
     5581 tcgaaagttc aaatctacct tttctactat ctactgctcc tgtaaaagaa cctttttcat
     5641 gaccaaacaa ctcagactcc aatagatttt ctgaaaaaga acaacaattt acaggtacaa
     5701 atatctcatt ctttcttgga cttatactat gtatatatct agctaatata tcttttccta
     5761 caccagattc ccccaagatt aatatattaa catctttaca agcagctttt tttgctattt
     5821 ttatgatatt attaaaatct ctatttttag attccaaagt aaattcaaaa ttattttttt
     5881 gtacagaaac agatatttta cttgccttta ccttttcaac ttcttctaat aaattttcaa
     5941 tgggattact ttttataaaa taagataaag ctcctctttt catagcatca actgcatttt
     6001 caatacttcc atatcctgta actaatataa cctctatatc tctattaata gatttaattc
     6061 tatctaatag ctgtactcca tccatatttt tcatcataac atcagataaa accaaatcat
     6121 aatttttatc ttttttaatt atatttatag cttcttcacc acttagtgct atatctacaa
     6181 gatagccctt cttttgtaga atttttttca ttaccactcc atattctagc tcatcatcaa
     6241 ctaatagtat tttcattata tagtcacctc tttatttaat ggaattttta tatgaaaaca
     6301 agttccaacc cccaatttac tttcaacact tatatctcca ttattctttt taatctcatt
     6361 ataagtaata tataaaccta aaccagttcc ctcaccaatt ggttttgttg tatagaaggg
     6421 attaaatatg tcctttaggg catcttcttt tattccttca ccattatctt taaaatctat
     6481 aaataaacaa tgattttttt cataacagta tataataatt tttccatctt gatgtattgc
     6541 atct
//
LOCUS       DAEPLF010000189         6518 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEPLF010000189 DAEPLF010000000
VERSION     DAEPLF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.189"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..841)
                     /locus_tag="KR314_002972"
     CDS             complement(<1..841)
                     /locus_tag="KR314_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBH0329338.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLS"
     gene            1137..2171
                     /gene="ltaE"
                     /locus_tag="KR314_002973"
     CDS             1137..2171
                     /gene="ltaE"
                     /locus_tag="KR314_002973"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBH0329339.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            2458..3288
                     /locus_tag="KR314_002974"
     CDS             2458..3288
                     /locus_tag="KR314_002974"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBH0329340.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGVLEV
                     "
     gene            3292..4173
                     /locus_tag="KR314_002975"
     CDS             3292..4173
                     /locus_tag="KR314_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBH0329341.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            4203..4820
                     /gene="gmk"
                     /locus_tag="KR314_002976"
     CDS             4203..4820
                     /gene="gmk"
                     /locus_tag="KR314_002976"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBH0329342.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            4823..5089
                     /locus_tag="KR314_002977"
     CDS             4823..5089
                     /locus_tag="KR314_002977"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBH0329343.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            5094..6293
                     /gene="coaBC"
                     /locus_tag="KR314_002978"
     CDS             5094..6293
                     /gene="coaBC"
                     /locus_tag="KR314_002978"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBH0329344.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLAFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
BASE COUNT         2600 a          686 c         1198 g         2034 t
ORIGIN      
        1 atgataaatc gctaaataat gtaaggttta tacaactgaa atcaactacc ttatccacct
       61 ttttatctat aataatgcaa ggggagtatt tgttagaatt tatatcattg aataaattac
      121 aaaatacttt gtgtaaagaa tcaaattgtt catcagatat atcctttatg atagcatttt
      181 gatttacacc tgctctgtag caaatttctt tagctataat agggcttata cctagaaatt
      241 tagaatatat agacttaaag ataggtcctt ccaattcttc taggttttta ataaacaaat
      301 ccttagatat atcatcaaga gggttgagtt tgttttgttc tggtggtaat gaataatcat
      361 gaccaggtaa aacttgacga actctactta tgctaaaagg gactcttttt atagaatcta
      421 taattttatt ttcttcacca tgagttatta ttatgttgct atgtcttccc ataatttcaa
      481 tcataatatt tttaactgtc ttttctttta attcatctaa agattcaaca cttattttta
      541 ttattctttc aaaacctatt tgagaaattt ctactatatt tccaccttgt atgtattttc
      601 taagtaacat acaaaacata ggcgctttta gagggttttc tttttgataa ttacttgtaa
      661 gataaactct aggatttgag gcacttgcac ttaaaaccaa tctaaaattt tctttattat
      721 ttcttatatt aaaaattacc tcatcatctt caggttgatg tatcttatct atttttccac
      781 ctgtaagttt tgatgataat tcatcgacta ttgaatgtat aactaatcca tctaatgcca
      841 tttatagcac ccctttcttt gaccaaaatg agtcagtaaa ttaattatac acataatata
      901 cgctttgtga ctagggggaa tttaaacgat agagattaat atttaaattg tataattatt
      961 tatacttatt agagaggaaa ctttattttt aaaattaata atttaaagta gattaaatat
     1021 aaagtaaata tagtataatt attgtgtaga gtataagcta tgaaagtata tgaactttac
     1081 agaaaaaatc aattttaagg ttctatagta tataaatatg aaagaaggtt gaaaaaatga
     1141 gatttatcga tttaagaagt gatactgtta ctatgcctac tgacgaaatg agagaagcta
     1201 tggcaaaggc tcctgttgga gattcaatct ttagagatga ccctactgta aataaacttg
     1261 aagaattagc agcagctaaa gttggaaaag aagatgctat tttccttcca agtggaactt
     1321 ttgggaatca attagctcta tttactcatt gcctaagagg gcaagaaata attattggta
     1381 aaggttatca cattgtaact catgaggttg gggcacctgc tgtaatagct ggagttcagc
     1441 ttagaactat agatgaagac gaaagtggag ctctaaatcc tgaacttgtt gaaaaggcaa
     1501 ttagaagaga tgacatacat gaaccacaga cagggcttat atgtgtagaa aatgcatatt
     1561 caggtggaac tgttgtgagt cttgataatt taagagaaat taaaaaaata gctgagaaac
     1621 acaacttacc agtacattta gatggagcaa gattatttaa cgcagcatta actttgggtg
     1681 tagaagctaa agaaatagca aagtgttgtg atagtgttat gttctgtgtg tcaaaaggtt
     1741 tagctgcacc aatgggttct atacttgctg gaagtaaaga atttatagca aaagctagaa
     1801 gaaagcaaaa agttatgggt gggggaatga gacaagtagg tatagttgca gcagctggta
     1861 taattgccat tgaaaaaatg actttaagat tgaatgaaga ccatgaaaat gcaaaatatt
     1921 tagcttgtga attaaataaa attgatggca ttgaagtaaa caatgttaat ccagatataa
     1981 gtatggtatt ttttaagatg agtgaagata ttataaaaga ggatgtttta ataaaagaat
     2041 tttttgaaag aaatataaaa attaataaaa tggaaaatgg tgaatataga tttgtaactc
     2101 atgtagatat tactaaggaa gatttagact atgttttaaa aacacttaaa gaattaatag
     2161 gtgtatgtta atagatatct actaaaagga ttatagctgg ttaccagatg tttaattaaa
     2221 aaattcatag acagtttaaa taaaacatac ataaaattca tatagaaata ttaatatata
     2281 aacatagaaa gaactagctt aaaaacataa tataattacc ctaattgtaa tgacaactct
     2341 ccctcacatc ctcaaattat agttgtcatt acaaaacttt tcccataaat tataagttta
     2401 tgggaatttt tttatgttat aatagtttaa gcgttaattt acgaaggagc gatacttatg
     2461 aaattttgga aactacatgg aataggaaat gattttatag caatagatgg aagattcgac
     2521 cagataaatc catctgatta tactgcttta gcaaaaaaag tatgccatag aagattttct
     2581 gtaggagcgg atggattgtt agttgtgaaa gattcaaaag ttgcagatgt agaaatggta
     2641 tattataatt ctgatggttc aagagcagct atgtgtggaa atggccttag atgtttttgt
     2701 aaatttgttt atgataattg tatagttaaa aaagaaagtt ttacagtaga tacattagat
     2761 ggtataaaag atataaaaat aaatcttacc aatagagaaa taaactctat aagagtcaat
     2821 atgggaaaag gaagttttat agctaaggat gttcctgtac ttacaaataa agaacgtttt
     2881 gtgcaagaaa gtataaaggt tttagatagg gagcttaaag ttacttctat gttgatgggg
     2941 gttcctcata cagtagttat agtagatgaa ttagatgtag atgatgtttg taaatatgga
     3001 aaagagattg aaaataataa aatatttcct gaaaaaacga atgtaaattt tgtaaaggta
     3061 gaagataagt ataatataca tgtttatact tgggaaagag gttgtggata tactttaggt
     3121 tgtggtactg gtatgactgc atcagcaata gtgtgcaatt tacttaatat ggtagaaagt
     3181 agtgtaaatg taacgtctca aggtggaaca gttaaaatag atgtaggaga tttctcttat
     3241 atgacaggtc cagcagtaaa aatatgtgaa ggtgttttgg aggtttaatt aatggctata
     3301 agtatgactg gttttggaag aggagaatac aaggatgata attattactt tttagtagag
     3361 tgtaaaacga taaatcataa gtattcagat ataaacatac gtcttccaag aaaaatttca
     3421 tttttagagg ataaagttag aaatttagta aaaaattatg taaaaagagg aagagtagat
     3481 ttatacataa agtttgactt attaggcaaa gaagatgtaa acttaaattt tgatgaggga
     3541 ttagcatctc agtatataga catactaaaa gaaataaaaa ataaatttga tattatagat
     3601 gatattagcg ttatgaatgt tgcaaagttt cctgatattg tgaaaataga agaaaaggaa
     3661 gaagatgaag atttattgtg gtcaatgttg aatcaagcag ttgaagatgc attaatcaaa
     3721 ttaagagaaa tgagaagtga agaaggtaaa aaattagctg aagatattgc catgagatgt
     3781 gatttattaa aaaatcatat agaagagatt gaaaaatatt caagtagtgt tgtagaagat
     3841 tatagagaaa aattgaattt gagaataagt gaattactgg atgacccaag tataatagat
     3901 gagaatagat tagcacaaga agttgcaata tatgcagata aaagcagtat aacagaagaa
     3961 atagttagat ttaaaagtca tatagggcaa cttaaaaata ctatatttaa agacgactct
     4021 ataggtagaa aaatagattt cttaatacaa gagatgaata gagaaactaa cactataggt
     4081 tcgaagtcat ctgatataaa tataactaat ttagtagtag aagtaaaaag cgaattggaa
     4141 aaaataagag aacaaataca aaatatagag taagaataca atattagaag gggaatgctt
     4201 aaatgtttgt gaaaaaagga ttattgctag ttgtatcagg accatcaggt gcaggaaaag
     4261 gtactatttg caaagaacta cttaaagaaa atgatacaat aaaactttct gtatcagcta
     4321 ctacaagaaa acccagaact ggagaagttg atggagtaaa ttatttcttc ataagtaagg
     4381 aaaagtttga agagatgata gaaaaaggtg agtttttgga gtatgctcaa atttatgata
     4441 atttttatgg aactccaaaa gcggcaataa tggaatgttt agaaaaagga caagatgtat
     4501 tactggagat tgaaatgcaa ggagcaaaac aaataaaaga agtttgccca gagggagtat
     4561 ttatatttgt actaccacca tcacttgaag aattaaaaaa tagaattgtt ggaagaggta
     4621 cagaaacaga agctgaaata gagaagagat ttagctgtgc atatgaagaa attaaaatga
     4681 ttaaggatta tgattatttt atatttaatg aagatgtaaa aacatctgct aaagaaatag
     4741 aaggtataat atcatctgaa aagaataaag ttagcagata taaaaatatt ataatagaga
     4801 aatttaagga ggaattataa ttatgttaaa accatctatc aatgaggtac tagaaaaaat
     4861 agacaacaga tactatttag tagggactgt ttcaaagaga gcaagaaaat taattgatgg
     4921 agaagaacca tatgtttcaa ataaaactaa ggaaaagcct gtatgtgtag caactaaaga
     4981 agttgcaagt ggaaaaataa catatagatt attgacagaa gaggaaatag agatagaaga
     5041 agctagacat catgctgagc aacaccaaca aatttcagaa gaggagtaag gtcatgctaa
     5101 aagataaaac tgttgtaatt ggcataagtg gcggtatagc tgcatataag gcttgtgatg
     5161 ttgtaagtaa gttaaaaaaa ctaaatgcaa atatccatgt tataatgaca aaatcagcaa
     5221 cagaatttgt taaaccactt gcatttcaat cgctgagtca aaattatgta gtagaagata
     5281 tgtttgaaga accaaaaact tgggaagttg aacatatatc attagcaaaa aaagcagatt
     5341 tattcttaat agttccagca agtgcaaatg ttataggcaa gattgcaaat ggtatagctg
     5401 acgatatgtt gacaactact gttatggcaa ccaaagcaca ggttcttata gcaccagcta
     5461 tgaatactaa tatgtatgaa aatccaattg ttcaaaaaaa tatacaaact ttaaagagct
     5521 ttggatataa atttattgaa ccagaaagtg ggaggcttgc atgtggagat attggaacag
     5581 gcaaattagc taatccagac ataatagtag aggaagtttt gactaatctt actaaagagc
     5641 aagatttaaa aggtcaaagt atgatagtta cagctggtcc aactgtagag agtatagacc
     5701 cagtaagata tttaactaat aggtcttctg gtaaaatggg atattctata gcaagagaag
     5761 ctatagctag aggtgctgat gtaacattaa tatcaggtcc tacaaatctt tctattccac
     5821 aaaatcttaa aaaatttgtt caaatagagt cagcacaaga tttgtatgaa gcagtagtat
     5881 caaatttaag tgaaaataaa atagtaattc aaagtgcagc agttgcagat tataaaccaa
     5941 aaacttattc tgacaaaaaa attaaaaaga agaatgatga tttatcaata gaactaagta
     6001 gaaattatga tattgcttat gaaataggaa aaataaaaga agataagata ctaattggat
     6061 ttgcagctga aactaatgac cttatagaac atgccaaagg aaaaataaaa aagaaaaatt
     6121 tagattttat tgtggcaaat gatttaacta aagaaggtgc tggttttaga gctgatacta
     6181 atattgtaaa aataatagat gtagagggaa atattactga gtatcctaag atgaaaaaag
     6241 aagaagtagc caatgttatt ttagataaag ttaaagcgtt gcttgagaaa taagaccttt
     6301 tttaaatatg atatttgtgt attttatcac agctaaagtg gctgaaagct gcaatttaat
     6361 tttagcgagt aaaaactcgc aaatgtcagt taaattgtag ttttcagcat ttattttgtc
     6421 ataaagtata aaatataatt aatagttaat tattaaaata aagttaatat taataacttg
     6481 aaattgctta taaattctga gattttaata tcttgtaa
//
LOCUS       DAEPLF010000190         6467 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEPLF010000190 DAEPLF010000000
VERSION     DAEPLF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.190"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            183..803
                     /locus_tag="KR314_002979"
     CDS             183..803
                     /locus_tag="KR314_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0329345.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKEAI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(1128..1952)
                     /locus_tag="KR314_002980"
     CDS             complement(1128..1952)
                     /locus_tag="KR314_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBH0329346.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     TLFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(1963..3366)
                     /locus_tag="KR314_002981"
     CDS             complement(1963..3366)
                     /locus_tag="KR314_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0329347.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIREYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(3370..4095)
                     /locus_tag="KR314_002982"
     CDS             complement(3370..4095)
                     /locus_tag="KR314_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0329348.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDVQNESKNLNKNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(4207..4980)
                     /locus_tag="KR314_002983"
     CDS             complement(4207..4980)
                     /locus_tag="KR314_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329349.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFLGFLLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVRYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(4986..5873)
                     /locus_tag="KR314_002984"
     CDS             complement(4986..5873)
                     /locus_tag="KR314_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329350.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDANYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVFDEP
                     TAGIDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSGDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(5887..>6467)
                     /locus_tag="KR314_002985"
     CDS             complement(5887..>6467)
                     /locus_tag="KR314_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329351.1"
                     /translation="ILSSKEKKKALKSKIFVALLVPLVIYSVYLMGIFIITCMQQGVP
                     VNGDLESFRIVDNVVALKTNLSITKYVILNIGIELLMFEGWAMIAIFGSFISKSSISS
                     ISFFIFIISITKIMSVIHILPKKLLSVVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNI
                     DIVNIAIGLLMAILIGAMSLCFLVIKSDYISR"
BASE COUNT         2280 a         1040 c          636 g         2511 t
ORIGIN      
        1 agaataattg aaagtcaatt ttataatagg aggtgtattt actaaaaaaa gagttatttt
       61 aaataggatt ctaagattag acatttctat ataaattgat aatattcatg aaaatcctat
      121 tttataaaaa ataatatctc ttagaaaatc tattaaataa aattcattgg aggaaattta
      181 taatgaaagt tttattgata aacggtagcc caaaccaata tggatgtact tatacagcac
      241 taaatgagat tactaaagta ttgagtaaac acgatattaa aacagaaata ctgtatttag
      301 gtaaagaagc aatcccagga tgtattagtt gcgcatcctg ttttgaaact ggaaaatgta
      361 ttcgaaatga taaagttaat gaattgattg aagatctcga taatattgat ggtattatta
      421 ttggttctcc tgtttacttt tctagtgcaa ctggtcaatt aacttctttt cttgaccgtc
      481 tatttttcat agctggaagt cgcatggcta caaaattagg tgcttcagtt gtgtcatgta
      541 gacgtggtgg tgcaagtgca acatttgacc aactaaataa atatttttca atcagcaata
      601 tgcctattgt atcatcacag tattggaatc aggtacatgg ctttactcca gaggatgtta
      661 tgaaagatga agaaggatta cagactatgc gaactctagg agaaaacatg gcatggcttc
      721 ttaagtgtat atcagcaggt aaaaaagctg gaattaaaga accacaatat gaagaaacaa
      781 ttatgactaa ttttataaaa taaataaaaa atgcactaat attaattaaa acaaaacact
      841 aatttataaa taaaataagt ataaatatag gataaaaaac actaaaacag aggtattgaa
      901 ttttatatca aacctctatt ttattattac attaataacc aaaccatata taacatcata
      961 tatttctaaa taaattataa tattatattc taatgttatt gaaattacaa taactgaatt
     1021 tctgctcata aatttaaata ttctacaaat ctcatctaaa taacaatttt aatagaccta
     1081 aaacaatcct catattgttc attttgtatt aaaaatatca cttacaatca aaaaccattt
     1141 attaagtata ttcctaagat atacaatata tatacatgga ttggatagta taaataaaat
     1201 aaatacttca tactcttccc tttttctcca ttgtaaagta acattaaagg tagagaagca
     1261 atcatcatcc attggtaatt ttgtaaaata gaatttttta aactataatc tccatttgac
     1321 attgttgtaa atataaatat acacatcaaa acataacaaa tactcaaact gaacttttta
     1381 cctctacaca aataaaaaat aatcccaagt ataatccaaa ttggtccacc ttctactaaa
     1441 aatggtactg gtaccaaaat cataaataaa gtaataactc tgttagtgac tttaaaaagt
     1501 aaagctactg taaatattcc taaaattata ggaatgatta tcatcaataa tcctataata
     1561 aaatatttaa tttctttctc ttttctaaat ttctttatga actctattgc ttgtaaataa
     1621 atcgctatta aaaataatgt tgaaaaaatg ttatttataa ttatagctcc ttctggatgt
     1681 gaaaatacat tattaattat ttgatttcct atagccataa taacagacca aatatacaat
     1741 ctacttatat atttttttct gttactagtg tgataaaatc cttcaacagc catgaatata
     1801 aacagtggtg ctgatattct gcctacccaa tgaaaccaaa ttggtatttg cattgtttgt
     1861 ggcataaatg ctgctatatg gtcaaatgtc ataaatatta atgctaaaat cttaatagta
     1921 aacccactta tacctttttt aaaaaaatcc atatcatatc tcctaatttt gttttatctc
     1981 tataataatt tttgttccta ttccaatttc actacttaca taaattttac ctttatgaat
     2041 ctctattatt tctttagaaa tagccatacc aagacctgaa cctttatgca tctcacctgt
     2101 attagtacct ctataatatt ttttaaaaat atgtttcaaa tcacttttgc ttattccaat
     2161 tccattatct tttattataa tttctatatt atcttttttt acaatttcta cacttatcaa
     2221 agttccttcg ctattatgaa ctattgaatt aaatataaga ttggttatag cccttctgaa
     2281 taagattgaa tctacatata cttcaattaa atcttcatta ctttcaaatt caatattcct
     2341 atttttatat ctattatcat ttaaaatatc aattattata tctctgacta aactgactaa
     2401 atttattttt ttcttgtcca gaactatagt attatttttt aatcgagtag ataaatttaa
     2461 atcatcaact aaatctttta tatacttaga cttattatat ataatttctg tatactctcg
     2521 tatttcatct tcttcaaact cataatcttt gtcattaatt atctctgcat acccttgtat
     2581 agatgctaga ggtgtcttta tatcatgtgt tatatttgcc agccattcct ctctcaactc
     2641 ttcattttct tttctctcaa cttcatttac tcttaaagta tcagctaaca tatttatatt
     2701 tttaaaaact tcttcatata tacctctgtc cttaagatat aaactataat ttccattagc
     2761 catagtctca atactccata gtacgcttga aattggtttt gtaagttttc tactaaataa
     2821 atatcctaca cctaaagcta ccacgctatc aattactagt gttataatga taactttatt
     2881 taaagttttt ttaatgttat tttgagaata ggttaaaatt attcttgata tatctctact
     2941 aggtatacct actattatgt ttagatgttt tttgtttaaa tatttttcta gtacaaaatt
     3001 aacatacttt gtttctatat acttgtaatt attcaccatt tgaaagggtg tatatttttt
     3061 tggagtatcc tttggcacat ttactccata aacttcctct cctaaatcat ttaaaacctg
     3121 tatccaggaa tttgattttt ctaatttttc agctccaatc ttagatagtt taaattctcc
     3181 atctttttca tataaatctt tttcaaaaga acgtgcaaat tcttcaggat tatttccaga
     3241 atctacaact ttaaaaaagc tattagttga aatcacatac aatatagata taatatttat
     3301 aattactact gatatagcta caaacacaat tgtaaatata aaatttctag taatcttcca
     3361 cttcatattc taatcttcct ttactgctaa tttatatcca agacctttta ttgtcagaat
     3421 atattcaggc tttgaaggat tatcctctaa tttttcccta atttttctta tatgaaccat
     3481 tatagtattg tcaaatccaa cataatcttc tccccacact ttatcccata gtttttcttt
     3541 acttataatt tgattttgat tatgtgcaaa taaagatagg atttttagtt cctttgcaga
     3601 cagtcctata gattttccat cttttataag ttctgcccta ctttcattta atatatatgg
     3661 gccaaatgat ataatttttt cttcattctt atttagattt ttactttcgt tttgaacatc
     3721 actaaaataa cttaatcttc tcaaatgcgc tttaactcta aaagccactt ccttagggct
     3781 aaaaggtttt gtaacataat catctcctcc aatcgctagt ccaagtaact tatctaattc
     3841 ttcggtttta gctgataaaa atattattgg agaattggag gtttttctta tttctttaca
     3901 aatatcataa ccttcgccat ctggtaacat aatatccaat acaatcaaat caggattaat
     3961 acttttaaat aattctaatc cttctttttt agtttttgca gtatatatat tattaaggcc
     4021 ctcttttttt aggacagttt ctaaaagctt caatatttct acttcatcat ctattattaa
     4081 aacacgattt tccataaaca acctcctgta ataatttaca tatatattag catatatttt
     4141 atctaaaatt catttatctt atttttaatt aaactaaaat ataaataaaa ttactattat
     4201 ttttagttaa aaccttttgc ttgatgtaaa attttcatat gttattttag ttaacactat
     4261 cattaatata attgagatta ctatagaaaa aactatcaaa atacttggta cattatacct
     4321 aaccatttgt tggctcatca taatccaatt tggagtcact gacataaatt ttatagcttt
     4381 atctggtata aaacctacac taaagtatgg aattgccatt atcaatccac ctagtataaa
     4441 tggaattaca gccgatttaa atatagatga aagtgttaag cctatttgtg caaatataaa
     4501 agaagcaaaa atagtagtca atataacaat tatatacatt tgtattactg taagatttaa
     4561 attactattt ccaaagtacc ataagttatg aatcggttca tttaaatttt taaaatcaaa
     4621 aaaactaaaa cctctaattc ctactaacaa tataaaagaa atattaataa tagataaaat
     4681 taatccatta gctactagtt ttgataatac tactgatttt tttccctttt tagttgtatt
     4741 tattaaacct gtcatattag aatcatattc tttaatgtaa tttccagaag ttattataaa
     4801 cattatcaaa cctattagag gtattgagcc ttctatcttg taaaatatat ctgcataagt
     4861 atctaaaaca tcaaactttc taatcagaag aaatcccaaa aatattaaaa acagccaaat
     4921 tatccaaaca ctctttgatt ttaatatttt tatcatttca gctttaacta aatacatcat
     4981 ttctactaca cctcccttgc atcttccaaa tcaaaataaa acatgtatac atcttcgaat
     5041 tttgcttgaa ctggttgtat attatcccca cttggaatat cctctgaaac aattctaagt
     5101 actactgaat caatatcact ttgaatactt acaaccttat atttagattg tatatcatca
     5161 acttcacatt catttttaac ttgaacatta tatactttat tttccatgtg acttaatatg
     5221 tctttgtgtg ttcctttcat tagaagtttg ccatctttaa tcattattgt ttcctttgca
     5281 actgattcta tatcggaaat tatatgagta gaaagtatta ttatcttatc tctagacatt
     5341 tttgctatta aattcctaaa tctagctctt tcttgtgggt caatacctgc tgttggttca
     5401 tcaaagacta ttatcttagg gtcgtttagt aaaacttgtg ctatcccaac tctcctcttc
     5461 atacctcctg aaaattttcc tatagactta tttctcacat cgtaaagacc tactaaatct
     5521 aataattctc ctatcttact atttaaatat tttcccctta aaccttttaa agctccaata
     5581 tactgtaaaa attcttttgc tgtaaaattt ttataaactc caaattcttg tggcaaatat
     5641 cctaaaatat ttctataatt agcatccatt ttaaatatat cctcaccatt ataaagtatc
     5701 tgaccttcat caggttttat caacgttgtt atttgtttca ttaatgtaga ttttcctgct
     5761 ccatttggtc ctagaagacc ataaactcca ttttccagag ttactgtaat cttgtcgttt
     5821 gctacttttt taccatatat ctttgaaaga ttttctattt ctaatttatt catcttaact
     5881 ccccttttat ctacttatat aatctgattt tattactaaa aaacataatg acatagcacc
     5941 tatcaatatt gccatcaaaa gtcctattgc tatattgaca atgtctatat tcactttaaa
     6001 tatcataata tcacctagat aatttcctat tattttatta aaaccaaata tcaaatcata
     6061 ataatttaca tttgatacaa ctgaaagtaa ttttttaggt aatatatgga ttaccgacat
     6121 tatcttagtt atagatataa taaatataaa aaaacttatt gaagatattg aagatttact
     6181 tataaaagac ccaaatatgg ctatcatggc ccatccttca aacatcaaaa gctctatgcc
     6241 tatatttaat attacatact ttgttatact taaattagtt ttcaatgcaa ctacattgtc
     6301 tactattcta aaagattcta agtctccatt aacaggtact ccttgttgca tacaagttat
     6361 aataaatatt cccattaaat aaacagaata aattactaat ggaactaata aagcaacaaa
     6421 tattttagac tttaatgctt ttttcttctc ttttgatgat agtatga
//
LOCUS       DAEPLF010000191         6440 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEPLF010000191 DAEPLF010000000
VERSION     DAEPLF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.191"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            253..1197
                     /locus_tag="KR314_002986"
     CDS             253..1197
                     /locus_tag="KR314_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBH0329352.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1219..1845
                     /locus_tag="KR314_002987"
     CDS             1219..1845
                     /locus_tag="KR314_002987"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBH0329353.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1879..2928
                     /gene="hisC"
                     /locus_tag="KR314_002988"
     CDS             1879..2928
                     /gene="hisC"
                     /locus_tag="KR314_002988"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBH0329354.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2931..3509
                     /gene="hisB"
                     /locus_tag="KR314_002989"
     CDS             2931..3509
                     /gene="hisB"
                     /locus_tag="KR314_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBH0329355.1"
                     /translation="MRIWKVERNTFETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3511..4116
                     /gene="hisH"
                     /locus_tag="KR314_002990"
     CDS             3511..4116
                     /gene="hisH"
                     /locus_tag="KR314_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBH0329356.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDITLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELVAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4113..4835
                     /gene="hisA"
                     /locus_tag="KR314_002991"
     CDS             4113..4835
                     /gene="hisA"
                     /locus_tag="KR314_002991"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBH0329357.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGINTKIIEDIANNIDIPIQVGGGVRDKGKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSDVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4817..5575
                     /gene="hisF"
                     /locus_tag="KR314_002992"
     CDS             4817..5575
                     /gene="hisF"
                     /locus_tag="KR314_002992"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBH0329358.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKNMGVEVRL"
     gene            5603..6271
                     /locus_tag="KR314_002993"
     CDS             5603..6271
                     /locus_tag="KR314_002993"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBH0329359.1"
                     /translation="MDNKCNNVYSDEVEEFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQKLWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMEPEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2627 a          542 c         1263 g         2008 t
ORIGIN      
        1 tatcatggtg agattaaata tagtaaaatt aaatacaaaa ttaaatcatc accatcatca
       61 tagattaaag ggattgtaat atgaaattaa ttcatagaca caagccctgt ttttagtgta
      121 attggtcttg tatctatgat gggaagtaat tttaggagaa aattatagcc atataggtat
      181 aaagcctata tggctttttt attgttttaa atttgcaaat ataagagtaa atttaatgaa
      241 gagggtgaga atgtgaaatt tttgaggatt gaagatgaag ttatttattc taaaaaaaga
      301 tataaattaa aaagggaaat agataaactt tttttggatg aagaatattt tcaaatagaa
      361 ccacaattat ttgaagaata tgatgaattt acaactataa ataataaaat tccagaagaa
      421 tctatggtaa aggttgtaaa tggaaaagta atggtactta gagctgatat aactacaagt
      481 ataataaaaa gccttgtacc aagatgggaa gatggtttaa agcttaagct tttttataat
      541 tcatcaatat ataaaaataa aaatacagtt ggaataaaag agataagaca aataggttgc
      601 gagtatctag gagaagcctc tgtagaagca gatagagaag tagtgaaatt agctcttaag
      661 atacttgaaa aatacaataa taattttatt ttagaggttg ggagtagcaa gtatattcat
      721 ggtttgttag aagaattaaa tttaaataag aattgtgaaa atcaaattaa aaatttacta
      781 tatacgaaga acactcatga attaaaagta tatattgaag aactgaaaat taagcgtgaa
      841 gtaaaagagc tattatcaaa tattttaagc ttgcaaggga atttatacaa cgttattgaa
      901 aaatcaaaca agttctattg taataataag atgaagcaag ctcttgaaga attaaagcaa
      961 gtaaataatt taattgagga atgtaatttt ttagataagg ctagatttga tttatcaatg
     1021 attactatgc ttgactatta tgaaggaatt atgtttagag gatactaccc aaactcatat
     1081 aaagaaattc ttagtggtgg aagatatgat tctttaacta aagagtatgg aaaagaaatt
     1141 ccagccattg gatttacact aagtgttgat gagttaatga agtatgtata taagtaaagt
     1201 ttagaaggag ggattgcttt ggattatttg actatagcac tagcaaaagg acgaattgaa
     1261 ggggaatcat ttaaaaaatt taaaaaaatg ggtctaggag atagtataga cacagatacg
     1321 agaaaactaa tttttaaaga tgaagaaaat aaaattatat atatacatgt aaagccatca
     1381 gatgtagtta catatgttga aaaaggagtc gctgaccttg gtatagcagg taaagatact
     1441 attttagaga atgagacaga tgtatatgaa atatatgatt taggttttgg aaaatgtaaa
     1501 tttgctgtag caggattaaa gggagatagc atatatagag aagatgaata tctaaaagtg
     1561 gcaacaaagt atcccaatat agcaaaaaag tattttaaag agaaaggtca aaaaattgaa
     1621 ataataaaat taaatggttc tgtagaacta gctccaatag tagggttgtc agatgtaatt
     1681 gtagatatag ttgagactgg caatacactt aaagcaaatg gtcttgaaat attagaagac
     1741 atatgtaata taagtgctag aattatatca aatagagcta gctatagatt taaatatgaa
     1801 caaattcaga atataattcg tttatttgaa gaacttgata attaaaatat aattgaactt
     1861 tataaggagg gatttaagtt gagagaaaaa gaaagtataa gagagttaag gggatatgaa
     1921 ccaaatcacg taaactgtaa agtaaaactt gatgcaaatg aaggaagtaa gaggttgttt
     1981 aaatacctta ttaaagaaat ttcagatagt gatatagatt taaatttata tcctgaagat
     2041 tcttatagtg atttgaaaga atctataata gattacataa atatttctgg agtaaataag
     2101 aaaaatcttc tagtaggaaa cggttcaagt gaaataattg atttaataat acatactttt
     2161 gtagataaag atgaagtaat attgagtttc tctccaagtt ttagtatgta ttctatatat
     2221 agtcagataa atgggtctaa atttataggt gttgaaagtg atgaaaattt agttataaat
     2281 atagataatg tgattgaaaa agtaaaggag aataatccta aaatagtaat tgtatgtaat
     2341 cctaataatc caactggaac aatattaaag agggaagaga taataaagct tttagattca
     2401 actaatagtc tagtggtttt agatgaagca tatatggatt ttggagaaga aagcatgtta
     2461 agtgatgttt ttaagtatga taatctaata gttcttagaa ctttatcaaa ggcatttgga
     2521 ttggctggaa taagaacagg atatatgtta tctaatagta gtttaataaa ttctgttgag
     2581 aaagtaagac ctccatataa tttaaattca ttatcagact ttattgctac aagagcactt
     2641 agaaataaag atgtggtaaa ggcttatata aaggaagtaa aagaggaaag agaagtttta
     2701 tatgaagaaa tgattggtat gggaattaaa gcatacaaat cacaagcaaa ttttatatta
     2761 ttttattctg aaatcgaaaa tttatcacaa aaacttattg atagaggagt tttaataaga
     2821 aaatttggtg gaaagttaga aaattattac agagttacaa ttggtgataa agaagaaaat
     2881 tctatgtttg ttggagctat aagagatatt ttgaaaaagg agaaataggt atgagaattt
     2941 ggaaggtaga aagaaatact tttgaaacac agattttagt agaattaaat attgatggtt
     3001 ctggtaaagc tgaaatagat actggaatag gatttttaga ccatatgctt accttaatgt
     3061 catttcatgg caagttcgat ttaaaagtga tttgtaaggg tgatacatat gtagatgacc
     3121 accatagtgt agaagatata ggtatagcta taggtgaagc atttaaaaat gcactaggtg
     3181 acaaaaaagg aatacgaaga tattctaata tctatatacc tatggatgaa tctctatcaa
     3241 tggtagcaat agatataagt aatagacctt atcttgtatt taatgctaaa tttgataccc
     3301 aaatgattgg tagtatgagt actcaatgct ttaaagaatt ttttagagct tttgtaaatg
     3361 aatcgagagt aacattacat atcaatctct tatatgggga aaatgaccat cataagatag
     3421 aaagtatttt taaagcattt gcaagagctt taaaagaggg tagtgaaata gtttctaatg
     3481 agatagcatc ttcaaagggg gttttgtaga atgaatataa tagtagacta tggtcttgga
     3541 aatatagatt ctgtgtctag gggttttaga aaagctggaa tagaaactaa aatttctagt
     3601 gatatagatg aaataaaaca ggcagattca cttatacttc caggtgttgg agcttttaga
     3661 gattctataa gtgccttaga taaactagga ttgataccaa taataaagga gcatgtttct
     3721 aagggtaaat ttatgatagg tatatgtcta ggaatgcaat tactttatga aaaaagttat
     3781 gagtatggag aatatgaagg attaggactt ataaaaggaa gtattgataa actggatata
     3841 actttaaaag tgccacatat gggatggaat aatctaaaat ttaataaggc aaatgatgat
     3901 atacttaaat atataaacga agatgattat gtatacttcg ttcactcata ttatgcaaat
     3961 tcatcaaatg aagaactagt tgcattttca gaatatgaaa agaaaatccc tgctattgtt
     4021 agaaaaggca atgtatatgg tatacaattt catcctgaaa aaagtggaga agtagggtta
     4081 aatatattaa gagcatatgg ggagatgata aaatgataat atttccagca atagatataa
     4141 aagataataa gtgtgtaaga ctcacacaag gagaatttga caaagtaaat gtatattatg
     4201 ataatccatt agaagtagca tataaatgga aaaatgaagg agcagaatat atacatatag
     4261 tagatttaaa tggtgctaga agtgaatttg gtataaatac aaaaataatt gaggatatag
     4321 caaacaatat agatatacct attcaagttg gtggtggagt tagagataag ggaaaagtca
     4381 aaagtttaat aaatgctggt gttactagag taatattagg tagtatagcg attgaaaatt
     4441 tgaatttggt tgaagaattg gtcaatgaat ataaagagaa aattgtagtt tctatagacg
     4501 ctaaagatgg aaaagtagca gttaggggat gggaagttgt aagcgatgta gactcattaa
     4561 cattatgtaa acaacttgaa aagataggtg tacaaactat tgtttataca gatatatcaa
     4621 aagatgggat gttgcaaggg ccaaattttg acatatatga aagaatagca aaggaaacgt
     4681 cattaaatgt tattgcttct ggaggcgtaa cttctataga agacgtaaaa agactaaaag
     4741 ctatgaactt atatggtgca ataataggta aagcgcttta tgataaaaag atagacttta
     4801 aggaggcaca acaattatgc ttactaggag aataatacct tgtctagatg tgagaaatgg
     4861 aagagttgta aagggaaaaa aatttaaaga tatagtagat gttgatagtc cagaagtact
     4921 tggaaaattt tatagtgatt gtggtgcaga tgaacttgta ttctatgata taactgcatc
     4981 aaatgaagag agaaagacat ctttagaatt tgtgacaaaa gttgcagaaa atataaatat
     5041 accattttgt gttggtggag gagtaaataa attagaagat ttcactgata ttttaagaaa
     5101 aggtgctgat aaggtttcta taaattcatc agcagtcaaa aatcctgaat taataaggga
     5161 agcttcatta aaatttggag cacaatgtgt tgttttatcg atagatgcaa agaaaaatga
     5221 agagggttca tggagcgtat acgtaaaagg tggaagagaa aaaactaatc tggatgcaat
     5281 tgaatgggct gttaaaggtg tagaacttgg agcaggagaa attgttgtaa atagtatgga
     5341 cgaagatgga atgaaaaatg gatatgatat agaattatta tcaaagataa cttctttagt
     5401 aaatgtacct gtcatagcat ctggaggcgc tggaaaaaag gaagattttt atgaggcagt
     5461 taataaatct aatgtagatg gaattttagc agcatctgtt tttcattttg gcgagataaa
     5521 aataaacgat ttaaagaaat atttaaaaaa catgggtgta gaagtaagat tataagttaa
     5581 atactaaaag gagattagaa atatggataa taagtgtaac aatgtataca gtgatgaagt
     5641 agaggaattt ataagaagta taaagtttga tgataaaggg ttagtccctg ttgttgttca
     5701 agaggtagtt agtaaagatg tactgatgtt agcatatatg aataaagatg ctataaagaa
     5761 aaccttaaaa gataaagtag cttgctattt tagtagaagt agacaaaaac tttgggttaa
     5821 aggtgagaca tctggtaata ctcaaaaagt agttaagatg tcttatgatt gtgatgtaga
     5881 tactatacta ctttttgttg aacaaactgg tgtagcttgt catacaggca attattcttg
     5941 tttttacaga gacttgtttg atgatagttc aaaaatggag cctgaagtac aaacaaatat
     6001 attgaaagaa ttatatgatt taatcaatga aagaaaaaat aatccagttg aaggttctta
     6061 tactaattat ctttttgaaa aaggaataga taagattctt aaaaaagtag gtgaagagag
     6121 tagtgaggta attatagcat caaaaaatac cgataaaagt gaacttattt acgaaattag
     6181 cgatttagtg tatcatacat tggttttaat gatagaaaaa ggtgttgaaa ttgatgaaat
     6241 aaaaaaagaa ttacttaaaa gaagaaaata gaataggata gattaatggt aataagaaaa
     6301 tataccgctt ttaataaaaa gtggtatatt ttttatttta tattttcgat ttatagaata
     6361 atatttaaat tacttgataa aattaactat atatataatt tattgataaa ataattgtag
     6421 tacattattt attttagaaa
//
LOCUS       DAEPLF010000192         6355 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEPLF010000192 DAEPLF010000000
VERSION     DAEPLF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.193"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            265..831
                     /locus_tag="KR314_002994"
     CDS             265..831
                     /locus_tag="KR314_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0329360.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            871..1452
                     /locus_tag="KR314_002995"
     CDS             871..1452
                     /locus_tag="KR314_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0329361.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1509..2426)
                     /locus_tag="KR314_002996"
     CDS             complement(1509..2426)
                     /locus_tag="KR314_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0329362.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2556..4187
                     /locus_tag="KR314_002997"
     CDS             2556..4187
                     /locus_tag="KR314_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0329363.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4190..5137
                     /locus_tag="KR314_002998"
     CDS             4190..5137
                     /locus_tag="KR314_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBH0329364.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALATEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5422..5685)
                     /locus_tag="KR314_002999"
     CDS             complement(5422..5685)
                     /locus_tag="KR314_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBH0329365.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5784..6029)
                     /locus_tag="KR314_003000"
     CDS             complement(5784..6029)
                     /locus_tag="KR314_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329366.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         2294 a          856 c         1004 g         2201 t
ORIGIN      
        1 tagaaatttt agaattattg agtctaactt tattacttac tttactttat aacaatcaaa
       61 tacaaattta aatattataa aactacaaaa taatttaaat catataatgt caatatgttt
      121 aaattataaa atattcatgt agttatataa taaataattt ataaatatag ccaacttagc
      181 ctaaatacgt atataatata gctaatatgg ttcttatact tgataccatc aataaaacta
      241 aaaatataaa aaggagaact taaaatgagt gttagtttac aaaaaggaca aagagttagc
      301 ttagtaaaag ataataatcc tgtaaaaaat ctagtagtag gcttaggatg ggatatgaat
      361 aaattgggta aaaaaaatta tgacctagat gcatttgcaa tagcattgac taaccaagat
      421 aaaatgaatg ttagaggtga cttggtttat tttggaaatc ttaaacaccc ttctaaagct
      481 attatacaca ctggagataa cttaactgga aaaggtgaag gtgatgatga acaaataatt
      541 gttaatttag aagacatacc tgaatatgta cataaaatag tttttggagt ttctatatac
      601 aaagctaaaa aaagagacca agattttggt caaatgaaca actcttttat aagattaatt
      661 gattcaaatt ccaagcaaga attatttaaa tataatcttc aagaagactt ttctggtaaa
      721 gtaacagttt tggcaggaga aatttatcgt agaaatgaag aatggaaatt taatgcttta
      781 ggaattggtc aaaatgaaga gcttcgtgaa ttaattaata catataagta ataattacaa
      841 taaaaaattt ataatgagga gttggtttaa atgggtataa ctttagcaaa aggacaaaaa
      901 gtaagtttaa caaaaagtaa ccctggtcta aaaaaagtaa tagtaggact aggatgggat
      961 attaataaat atgatggtgg atttgatttc gatttagatg cctctgcatt tttaactgga
     1021 actgatggaa aagtaacgaa tgatggagac tttatattct ataataattt aaaacacgct
     1081 tctggagctg ttgaatatat gggtgataat agaacaggtg ttggtgatgg tgatgatgaa
     1141 caaataaatg tagacttatc taaaatacct caaaatattg ctaaaatatc atttagtgtt
     1201 actataaatg aagcaattac aagaagacaa aatttcggac aagttgaaaa ttcatatata
     1261 agaatatata atgaagaaac taacgaggaa cttattaaat atgaattggg tgaagatttt
     1321 agcatagaaa cagcaatagt cgtggcagaa ctatatagac ataatggaga gtggaaattt
     1381 aatgctttag gttctggatt tgaggatgga cttgcaggat tgtgtaaaaa ttttggcgtt
     1441 aatataggct aatatatcta aataaaaata agagtatatt gatatactct tatttttatt
     1501 tagatatatt ataaactgtt tacacttcta ctcttctttc tcgcttgttt tttcccatat
     1561 tctactgaaa tatcaaaaaa tgctgaaata ttttctgtaa aaaacttgct tctatgatat
     1621 gcaatatcga atgttctatg taagttaata ccttcaatat cacaagccca caaaagatgc
     1681 ttttttaaat aatcctcaat aagtaattcg gatataactg caatgccaaa attatctaca
     1741 actgctctta atatagcttc agagttataa catgaccatc taatattcat tgggattttt
     1801 aattctttta attgcttttc taattgtgct cttgtaccac ttcctaattc acgcataatt
     1861 aatggttgat ttgaaagttc tgaaactttt atagagtctc ttttgtagaa ctcatgcttg
     1921 tgtgaacata taagtacaag attatcatta ataatgcttt ttgtaacaat atctgggtgc
     1981 ttaatttttc cttctacaat cgcaatatct aattcactat taagtaattt ttttgcaatc
     2041 agtcttgtat cttctacaaa tacttcaggt tctataagag gatttacctt gtataattct
     2101 aacattatcg ggcttattac acatgtacca acagttactg ttgccccaac tcttatacgg
     2161 atattttttg cacaatgctt catacaatca ttcatttctt catttacagc taaaagatgc
     2221 ctcccatatc caagaagttt tttccctgcc tcagttaaat ataatttttt tgaaattcta
     2281 tcaaataact taacaccata ctctttttct atttctgaaa tagcttggct aactgatgac
     2341 tgggtaatat acatatttct tgctacttca ctcattttac cacattcagc aaccttaaca
     2401 aagatttcaa gactacgaat tgtcatatta tcacaacctt agcaaacatt agtaaatgct
     2461 tatgatttta ttaatatttt agtattttac aaatatattg tcaagaagta taattttatt
     2521 atagagttat aaagtagatt ggagtgtgat atattgtgaa agtattaatt ataggtggag
     2581 ttgctgctgg aacaaaaact gctgcaaaaa taaaacgtga aatgggtgat aattgctctg
     2641 ttacaatact aaataaaggt gatgatattt cttatgcagg ttgtggtctt ccatattacg
     2701 ttggtaagat aattgaagat aaatcatctt taatcgtaaa tacacctgaa agtttttcta
     2761 aactaacagg agcagaagtt cgttgtggag ttgaagttac gtctattgat agaaatgcaa
     2821 aacaagtaac tttgaaaacg ctttcaacag gagaaaaaga aaatctaagc tatgacaaac
     2881 ttgtaattgc tacaggggca gacccaataa aaccaccaat acctggaata gatttacctg
     2941 gagtattctt tatgcgtact ccaaatgatg ctataaaact aagagattct attgaaaacg
     3001 gtattaagag agctgttgtt attggtggag gtttcattgg tcttgaaatt gctgaaaatc
     3061 tttctgcaat gggtatacgt gtaagtgttg ttgatatggc tgaacatgtt atgcctggat
     3121 ttgatactga ttttgctgaa tacgttgaaa atcatatggc tgataaaggc ataatgattt
     3181 ttacaggtga ccaggttgta ggtattgaag gtgaaaataa agttgaaaaa cttcgtacta
     3241 aaaatagagc tattaaaaca gatttagtgg ttatgtcagt tggaatccgt cctaacacag
     3301 gttttctaaa agacacaggt ttagaatttg cccctaataa tacaattatt gcagatgaat
     3361 atatgcttac aaatgataaa gatatatacg tagttggaga ctgtgctttt gtaaaaaatt
     3421 ctttaacaaa taaacctaca tggtcaccta tggggtcatc tgcaaatcat gagggtagaa
     3481 tttgcgcaca aaatatatct ggaaaatcta aaacttacaa tggtgttctt ggaacgacag
     3541 tagtaaaatt acctgagctc aatgcaggaa aaacaggtct aagtaaagaa atagctgaaa
     3601 aagaaggtta taatgtttgc tctgtaacca tagctaccga tgataaagct cattattatc
     3661 caggtgcatc taattttatt ataagaatgg ttgctgaaaa aaatactaga aaattattag
     3721 gtgttcaagt tatgggacct ggagcggttg ataaaatagt agatatcgct gctactgcaa
     3781 taactttagg cgctgacctt tattcattag aaagtatgga ccttgcatat gcaccacctt
     3841 tttcaacagc tattcaccct tttgtagtgg cagtaaatgt tttacaaaat aaacttaacg
     3901 gagaacttga tagtgtttta cttgacgaaa ttgaggattt tgacagttgg actaaacttg
     3961 atgtttcaaa agctccttct attcctagtt tacgttatat tcctgttggt gaaattaatg
     4021 gagaaattga aggtcttgct aaggatgaaa aaattcttct tatatgtgct aaaggtaaaa
     4081 atagctatat ggcgcaaaat cgcttaagac gctttggcta tacaaatacg aaagttcttg
     4141 aaggtggaat actattttat ccaaatctta aggtaatgga ggagtaaata tgagtaatgt
     4201 tacagcagaa caaaaaaagg aagtaaaagg tagaggtttt ctaccaagca gggatggaga
     4261 acattttgca gcacgtataa taactgtaaa tggtgtaata aatgcatcac aagcaaaaaa
     4321 aattgcagaa gctgctgaaa aattcggaaa cggtcagtta gcatttacta ctagactaac
     4381 agtagaatta cctggtgtaa aatttgaaga tattgaagct ctaagtgaat ttattgctag
     4441 cgaaaatcta ataacaggtg gaactggatc aagggttcgt cctgttgtgg cttgtaaagg
     4501 tactgtttgt gttcatggtc tagctgatac acaggcactt gctactgaaa tacacgaaga
     4561 attttacaaa aattggtatg atgtaaaact acctcataag ttcaaaattg gtgttggtgg
     4621 atgccctaat aactgtatta aacctgacct aaacgatctt ggaattgttg gtcaacgtgt
     4681 acctgactat gattcagagc tttgtgttgg ttgtaaaaaa tgtgctgttg tagaggtctg
     4741 tcctgttaaa gcagctaaat taacagataa aggtaaactt gaaatagaca gtaatctatg
     4801 taataactgt ggtaagtgta tagaaagttg taattttgat agtattgaag aaaaagaatc
     4861 tggttataaa gtctatatag gagggaaatg gggaaaatca gttcgtcctg gaacacagat
     4921 tgatagatta ttttcaaaag aggaacttat gactttaatt gaaaaagcaa ttcttttatt
     4981 tagagaacag ggtaacacag gtgaacgttt tggaataact atcgatagaa ttggtgctga
     5041 taaatttata gaaatgcttt tatcagatga agttttggaa agaaaagaag agatactaga
     5101 ggctctttta caccttacag gtggagctgt atgctaacaa taaatatttt aattgtacag
     5161 ataacctatg gaaaaaattt tagttaagaa taattaaaat tctatatatg atatatgata
     5221 aatgaggcta tctcaaaaca cagatacgcc tctttttcat tgttgaaatg ataaagtata
     5281 tttgagttta tacaaaaaag agtgtctcgt gaattataaa attcattttg aggcacccac
     5341 attattttat aataattgct tattaatttt attttaacta tataaaaagt acaaaaagta
     5401 agaatatcca atattctaaa attaattttg aattgtattg attgaaaagt ttactattcc
     5461 actatttcca tcttgattta ctactagata ccatgttcca gtattaggaa ctctcattct
     5521 acatggattt ttctttgcaa atccaccata gtaatcataa tttctttgat ttttaaattt
     5581 cacataattt atatggtcta acaatattac attggcttgt ttctctaaat ttacctctac
     5641 aatatcccct ttttttaagt tcatcttact ataactatat tccattgaat tactcctcca
     5701 gtatcgaata atctttgtta tttactcatc tcaaatatgt aaaaattcat ttaaaatatt
     5761 acaaatacta tataattaga ctattatcca gttttaacct tatttactac aatccaaagg
     5821 ttattaaaaa tattattggc aacaacttct ccatcccata caactctttc ttctttattt
     5881 tctaataaag catagtaccc agagtcagaa ctttcatctt tatagactgt aacataaaac
     5941 tcttcatcac tatattcagg tatattttta aagtcttcag atattttaca tgttaatata
     6001 tacccttcta attcttcatc tataaacaaa gcaattcctc caatctctat atttttacta
     6061 attataccat attatgtcat aaacttttta tttcattaca aatttctatt ttttctttac
     6121 tttataaata aattatataa attttcactt tttaacttta tattatacta attaattctt
     6181 ttgttttctt atatatttct ttataattta taaatttagc cattaattta tacgatttta
     6241 tataggtaag catatactct cacttaatat taaataattt acattttttc tatactataa
     6301 tcatattctt ttgtcagtta gtaattttca aagtagatta tttcaattaa aatat
//
LOCUS       DAEPLF010000193         6213 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEPLF010000193 DAEPLF010000000
VERSION     DAEPLF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.194"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(41..1105)
                     /locus_tag="KR314_003001"
     CDS             complement(41..1105)
                     /locus_tag="KR314_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBH0329367.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            1367..2038
                     /locus_tag="KR314_003002"
     CDS             1367..2038
                     /locus_tag="KR314_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0329368.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKACLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            2040..2948
                     /locus_tag="KR314_003003"
     CDS             2040..2948
                     /locus_tag="KR314_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0329369.1"
                     /translation="MFILAFIICTVVIFIVSTKINQRRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKSEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            3025..3711
                     /locus_tag="KR314_003004"
     CDS             3025..3711
                     /locus_tag="KR314_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329370.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            3708..>6213
                     /locus_tag="KR314_003005"
     CDS             3708..>6213
                     /locus_tag="KR314_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329371.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYIVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETAMEGFVNDYLQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKRGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTGEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIP"
BASE COUNT         2444 a          734 c          957 g         2078 t
ORIGIN      
        1 cctatgataa attctagtac agattcttta ttaaataaaa ttatttcaac caagatggat
       61 ttccaggtat aaatattctt ttaccaaatt ttgaagtata ctcatctaaa gctttttgaa
      121 taagctcatt attaccctta aatgaagtag tattctttat agtttcgaga ttttggctaa
      181 atgggcaagc agatatacac ataccacaat ctgtacctaa atatctccat ttaatatagc
      241 atgtctcatg ttcaattacc caattatatt ttccatcatc accaatttta atatcattag
      301 aaagcgaatg agaagggcag ttaaaagagc attttttaca gaccttacaa aaatcttcta
      361 gtccaaaatc aacatattca tcttctaaaa gaggaatatc tgtagtaaca acacctaatc
      421 taagtctaga gccataatct ttatttgtaa gtatagcatt tcttcctata tctcctaaac
      481 ctgcatcacg agctataaaa acaggcatta ctaaataatt agcatccata tgatttctag
      541 catcatatcc taaatttctt atatagtaac ttaaaatcat gccgacgata gatgcatcca
      601 catagcactt tgatgtttct ataacttcac atatcattgg agctctattt atcatttctt
      661 tatccatctc acatccaaat acaattgcat acttatgagt tagattaatt tcttcaccgt
      721 aattttcttc atgtctaccc ctatttgtat aaatatgata gtcttttaat ttagttatac
      781 caactacaga agctccatac tgctttgcaa gacccttaat tttttttgtc attgactttg
      841 catctacatc tactttagta tcagaaactt ttccttcgca taaagatttg atatcagata
      901 aaaaatcgaa tgcactactt gccattggag agtttaactc attgtaagtc attgttcctt
      961 cactacataa atttggtcta tttcttatac tatcatcaat ttcttttttg tcaggatttt
     1021 tcttataata atcattataa gcagtactat cttttttgta atttgctctt gcaaacatag
     1081 tatttctctc atcaaccctt ttcaattcta ttccccctag taaaaatata tttaaaaaat
     1141 ataactttat tgttgtataa tttaattata taagaataac gttatatttt caatttaagc
     1201 gtaattaaat tgggaaatat ataactatat gcttgattat gatgttttta ttttgaaaac
     1261 aaatttaaat acaatctttt tagtgaaata aataaaatta aaatattttt agtatgataa
     1321 tgcaagaaaa taattattta tctaaaaaat ctaaaggaga gtaaaattga acttattaat
     1381 tattgaagat gatataaact taaatgaagg tcttttttat gcatttgaga atgatggatt
     1441 taatgtattt aaggcttata caaaacaaga aggattaaat atatttaata gtaaaaatat
     1501 agattttata atcttagact gcaatcttcc agatggagat ggatttgatg tatgtcaaat
     1561 aataagggaa aagtcagata taccaattat aatgcttact gcaagagact cagaaattga
     1621 tgaagtaaaa ggattagaaa tagggcttga tgattatatt acaaaaccat tttctttatc
     1681 tgtattaaag gcaagagtca aagtagcaat aagaaaaaaa tcgaataata aagtaatata
     1741 ttccaatgga ataaaattgg accaaaagtt actaaaagta tataaaaata aagcatgttt
     1801 ggaacttagc tctgtagaat ataagcttct aagttatcta attgaaaata aaggtcagat
     1861 attattaaag gaacaaattt tacaccatat ttgggacagc gaagaaaatt atgttgatga
     1921 taatattgta tctgtaaata taagaagact tagagtaaaa gtagaggatg acccatctaa
     1981 tccaaaatat ataaaaacag cttatggaat gggttattta tggaatgagg tagaataata
     2041 tgtttatatt agcctttata atatgtacag ttgttatatt tatagtatct actaaaataa
     2101 atcaaagaag atatgatgaa ttaaatttga tgttaaatca aatattggaa ggtaaagaag
     2161 ttacttatcc agatacgaag gatacaagag cgtcaaaaat ttctcaccaa gtaaaaaaaa
     2221 ttaaggatat gatagaaata gaggtagaac agtcaaagtc agaaaaagaa gcgattaaag
     2281 gtttaatatc aaatatgtca catcaattaa aaactccact ttcaaatata accatttatt
     2341 gtgaacttct ggagaatata aatatttcca cattacagaa gaaggagttt ttacaaaaga
     2401 tgaaaaatga aacttttaag attgactggt tgttacaatc cttatttaaa atgactaaat
     2461 tagaggatgg agttatagaa tttgaagtag aagaactact aattaaagat actcttattc
     2521 aaagcatcag tactatcttt aataaatcag aagctaaaaa tataaggata aatctcgaac
     2581 cattttcaga tataaagtta gttcataata aaaagtggac catagaagca attgtaaatg
     2641 ttttagaaaa tgcaataaaa tactcaccat cagatagcac gattactatt tctgtaataa
     2701 aaatggagct atatacgaag attactatta aagatgaagg aataggtatt gatagtagag
     2761 aactaaatga tatttttaaa agattttaca gaagtaaaaa tgtggaaaac caaaatggca
     2821 cagggattgg tctatatctt acaagattaa ttttagaaaa agaaaatgga aacattatag
     2881 tagaatctaa actaggaagt ggatgttgtt ttagtatatt cttacagaat tgtaagagtt
     2941 taaactaact ttgtaggatg agtgtaatta tcgtcactta caatatttta tagaacatat
     3001 atctgtattg aaaggagaaa ttaaatgagt atattagtga cgaataattt agtgaaacac
     3061 tatggaaaag gtgagactaa agtaaaagcc ttaaatggag taagtataga aattgaacga
     3121 gatacattta ctgctatagt tggaacttct ggttctggaa aaagcacatt gttaaatatt
     3181 ataggtggat tagataatcc aacctcaggt gatgtaataa ttaaaggaaa gaacatatct
     3241 aaaataggaa aaaaagattt gacagtattt agaagaagaa atataggatt tatatttcaa
     3301 aactacagtt taatgcccgt attaaacact tatgataata tagctcttcc tgttacatta
     3361 gataaaggaa accatataga ccatgaatat attgaaatgc tgatgaagac attgggaatt
     3421 tgggataagc gtttaaaatt tccaagcgaa ctatctggtg gtcaacaaca gagggtagca
     3481 attgctagag ctttggcaaa taaacctgca cttatattag cagatgaacc aacaggaaat
     3541 ctcgatagta aaactacaat ggaagtggta tgtttattaa aagaaagtag tgcaaaattc
     3601 catcaaacga tacttatggt aacacacaat gaaaatatag cacaagtatg cgactcaatt
     3661 atccatattg aagatggagt tgtggtgaat actggtggtg aagtactatg atgtctctag
     3721 taaaattttc aattcaatat ataaagcatt ataaaaaaca atctatatct atagttttaa
     3781 gcatagtact atcagtagca ttattgacgg gaattggttc tttagttcat agtgcagaca
     3841 aaagtagaat tgaaaagata agggaagata gtggagatta tcattatttc tataaagtag
     3901 ataaggaaca acttaataaa attaaaaaaa ataaaaaatc aaaggaatac attgtagata
     3961 gacttggcgt tacaaaacct aaaaatcaga tagatgaacc ttttattata gaatttttaa
     4021 atgctgattc ttcatattta gacatgacag gtagaaaatt attaaaagga actcttccta
     4081 gtaaagatgg agagatagta cttgacacat attctctaaa caatttaaat gtaaacaaag
     4141 aaattggaac agaaatcaat ttagatggta aaatctataa gttatgtggt attttatcaa
     4201 gtctttcaaa cccagaaact gctatggagg gatttgtaaa cgattatttg cagcctaaag
     4261 aaaaggattc ttatatagtt tatgtaaaat atgatgaaaa taaaaatatt aaaaagcaaa
     4321 attataaatt tatgaaaaaa ttcaacatat caaaagaaaa aacttatata aactggcctc
     4381 tttcagttga atttggagta aaaccaccaa agaatataga aaaatataat atatttgagt
     4441 tcttaaaaaa tttagaacta aatacaaatg caattatatt gcttgttgga gtgtttggtg
     4501 cagcagcagt atatagtatt ttccatgttt caatacttca gagaatatca caatatgggg
     4561 ttttagaagt attaggcgct aataataaac aacttttatt tctactattt ttagagttat
     4621 ttttactttt tattgttgga tttcctatag gttgcttctt aggtattgga gtagcgtcta
     4681 caatatatga acaatttcca catatatttt tgagtagtga tattgttcca ggagcatttt
     4741 ttataagtga aaaatctatt tatttaggct ttttatttat cttattgttg ttaattttaa
     4801 ttgctgtacg tgttgtacat cagttgaata aatactctag tatagaatct atgaaaaact
     4861 ttaagattac taataagaaa aagagaatat tagaaagtgt aaaacactct aacatgacaa
     4921 aagtattatc tcataaatac atgactcaaa aaaggggaat gtttattgga attttgtttt
     4981 cattatcact tggtggaatt atatttctat gttctagtta ctctatacag cttactaaaa
     5041 acaataatga acttacaatg aaagctgatg atggtttaaa ttctgactac caaataacta
     5101 tgcaaacaac agattttgat gtaggtatac caaaagataa aatttcaaga ttaaacaatg
     5161 ttgagggaat atcaaaagtc tatccagtaa gatatttatt tggagctatg caaattaaag
     5221 aagaacaact attttggaaa aatttcttta aacctcttga aaaagattac aggataaaga
     5281 ctttttttga tggtatttgt acaaagcaaa atgaaggtgg ctatctttta aaaacaaata
     5341 tttatggtta taacaatggt atgttagaag gattaaattc ttatattata gatggggaga
     5401 ttaataccca tgatatggtt aaaaataata aggttatagt tagactacct atggatggaa
     5461 ctgggatgta tgatgcagtt gatataaatc caggagatac aataaaagtt aaagtgccaa
     5521 agacaatgaa accaacaggt gaaattgtta aatttaaaga agaaaataag aatgattata
     5581 caacaaaaga atttgtagtt gctgctactg taaaacgtgt gatggcaaat aacatatatt
     5641 tcattggtga ttatggtatg gatatagtta tgacgaatgg gcaaatgaaa aataactttg
     5701 atatcgaaaa ttataattca gtaagtataa aaaagacaga agatagtaac agtgaggaat
     5761 tgtctgagaa cataaaaagt gttgtaagtg atgtaaaaag atgtatcgta acagattata
     5821 ccattgctat tgaaaaaaat aatacctact taaatcaaaa actcttattt atatatggta
     5881 ttgtatttgt acttcttgca ataagtttgt tccatataat caatacagta agctacttaa
     5941 ttttttcaag aagacatgag tttggaattt taagagctat gggtattacg gataataagt
     6001 ttttacttat gatgataaga gaaggttttt tatatggatt ctatgcaagt attataatgg
     6061 taattggaag tgttattgga caatttatga tttattttat ggtaaaacgt gtatatttat
     6121 atataaatcc aattcttaaa ataaatacac ctctatacat aggtatgatt attttaaata
     6181 tcactattag tatagttgct gtaattattc cag
//
LOCUS       DAEPLF010000194         6204 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEPLF010000194 DAEPLF010000000
VERSION     DAEPLF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.195"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            70..2156
                     /locus_tag="KR314_003006"
                     /pseudo
     CDS             70..2156
                     /locus_tag="KR314_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
     gene            2171..2458
                     /locus_tag="KR314_003007"
     CDS             2171..2458
                     /locus_tag="KR314_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0329372.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            2485..3756
                     /locus_tag="KR314_003008"
     CDS             2485..3756
                     /locus_tag="KR314_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBH0329373.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            3814..4413
                     /locus_tag="KR314_003009"
     CDS             3814..4413
                     /locus_tag="KR314_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0329374.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIVLQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            4418..5323
                     /gene="dapA"
                     /locus_tag="KR314_003010"
     CDS             4418..5323
                     /gene="dapA"
                     /locus_tag="KR314_003010"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBH0329375.1"
                     /translation="MKYKGIICAMITPFDEKQNINSQATCKLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVIDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEVTQNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            5429..6061
                     /locus_tag="KR314_003011"
     CDS             5429..6061
                     /locus_tag="KR314_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBH0329376.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINLENLHIWLENGVDVCGFGGLLTKGTIEDITENAKKIREIIKNIREI"
BASE COUNT         2281 a          741 c         1160 g         2022 t
ORIGIN      
        1 gtattgtgga ggtgatgaaa gaatccaatg ataatatgaa gactaatctt aataagaaag
       61 taggggttaa tggtagtgga caaagaaatt gtatcaattc tccagatttt attaaatagc
      121 acattgatta caactaatgg attacaagag gagtctaatt ctcaaagaga caaattactt
      181 atagaataaa taaaatcaat gacatgctta aagtaaaaaa tgtaccactt atatgtttaa
      241 gagctgataa agatattatc gttagaaaag aaacaaaaaa agcaattaaa gaaattttag
      301 aaaaaaacta ttctaagaat acttattatt ttagtaagac agaaagactt ttatatatgt
      361 atttaatgct ttttattaac atggaggata tttcaattaa tcattttata aattctataa
      421 aagtaagtag gtctactgta aatcttgatt tcaaggattt gataccagag cttgaaaaga
      481 agaacattaa agttaagaac aatcgtataa atggatatta catagttggc aatgagatgg
      541 aaattaggag agtgcttatt aaaaacatta tagaaacttt gtctaatgag aaaagttcaa
      601 gagtatttga tatttttatt aaagaatata atttagatag ttttgaagag gctaaaaaaa
      661 ttatattaaa gctaataaaa aaatatgaaa ttacttttgt tgaagataga ttaatagaat
      721 ttatatatat atttatcttt ctaaaagcaa ggatgtattc aactagaata gttactcagg
      781 aaaatgtaga tattcctaat atttcagtta tgagttcaat gaaggagtat aaatttgcta
      841 aggaattatt agaagtatat gggagtgagg ataagataaa gccttataat ataatgtata
      901 ttagtgcttg gattttaggt atatcggtag gaaattatga agaagataca gaagataagg
      961 atgtaatttc aaaaattgta aataaaatga tgacaaagtt tggatattta agcggagttt
     1021 actatataag ccaagaaaaa atatttaaac aattatattc tcattttaga cctgcctact
     1081 atagactttt atttaaatta cctatctata atccgatttg tgaaaaagta aaagaagaat
     1141 ataaattagt ttatagaatt gtaagtaatg caatgaaaga gttttgcgga ttatttggag
     1201 aagaaattca ggaagaagag ttagcatatc ttacaatgca ctttgcaaca cttttttcaa
     1261 ataaaaaaga atttgatggt tttagaaaaa gaatagcatt aatagtttgt tcaaatggcg
     1321 ttggtagttc agctatttta catgcagaac ttacaaactt atttcctgac ttacacttta
     1381 taccaatgtc ggaatcagtt ggattagaaa atataagtga acctgtagat atcgtattta
     1441 ctactaatta taacgcagag ggtttaaaaa cagatgtacc agtaattaaa gtaagccctg
     1501 ttatgaccac aaaagaaaaa tataagatta cacgtgaagt gtatattcaa ttaggtgata
     1561 tttatttaag acaacctaaa atagatgagg ttatggatat aataaaaaaa tatgcaaatg
     1621 taacctctga aagtattctt tcaagtgagc tattagctta ttttacacag atagagaatt
     1681 ttacatctaa ggaaggagag gggcctatgc taagtgagat tactaatgaa acactagtaa
     1741 aaataaaggt aaaagctaag aattgggagg aagctattag acagtcagct tctgtattag
     1801 ttgaaaataa caaagtaact gaagggtatg tagatgctat gattaactca gcaaaagcat
     1861 ctggaccata catagtaatt acaaaacatg ttgcacttcc acatgctaga ccagaagctg
     1921 ggtcaaaaga gattgcaata ggcattgcta ctttagatag tcctattgag tttgggaata
     1981 acgaaaacga ccctgtaaaa tatgtatttt gtctaagtgc aatagacaat aatagccatt
     2041 tacgtgctat gtctgagcta gttgaactat tggaagaaga tgagtttttt aaggttttag
     2101 atagtgctaa agaggctaaa gaaattattg attttataaa aacacacgaa ttataaagga
     2161 ggatttaatt atgtataaaa ttttaatcgc atgtagagca ggagttggct caagtttaat
     2221 gttaaaaata aagactcaac aaattattaa ggaaaataac tttccaattg aagtagaaca
     2281 tggttcattg gattcactaa atggattcaa tggagatgct gtagaaacat taattgatgt
     2341 tgctgaagaa ttaaaaaata aaaatttgaa gtttgatata attgggattc acaatattgt
     2401 tgaccgtaat gaaataaaga cagcactaga aaaattcctt gcttcaaaag agggataaat
     2461 ttaatatgat aaggaggaat atttatggag tttattatta gtttgctgag tacacctgct
     2521 gttttgcttg gcgtagttgc atgtatagga ttgattcttc aaaagaaatc agccgttgaa
     2581 gtatttacag ggtcttcaaa aacacttatc ggttttttga tatttggtat tggtgcctct
     2641 gctatgacag gagcacttca aaatttcaat aaactgttcc aacatggctt tggaattact
     2701 ggtgttatag cttcaccaga agcagctaca gcacttgcac aaggtacata tggttttgca
     2761 gtttcatgta cattaattct tggattcata ttaaatttag tatttgcacg tattacaaag
     2821 tttaagaata ttttctttac aacaggtcat agtttattct tttcttgtgt attagtttta
     2881 atcatgaaag cacatggatt tgataataca atcactattt taataggtgg aactatctta
     2941 gggtttatgt ctgctgcact acctcaattc tgtcaaccat ttatgcgtga attaacaggt
     3001 ggcgacgaac aagctattgg tcactttaac atgattggtt atggtttatc aggttatatt
     3061 gggcgtttat tttcaaaaca taaagatgat acaacagaga ctatagaatt tcctaagtgg
     3121 ttatcaatat tcagagactt cttaatgggt ctatccatag ttatgttaat cttattctat
     3181 attgcaacat taaaagctgg tcaagcattt actcaagaaa ttgctggttc aacacattgg
     3241 ttggtttttc cgcttataga ggcatttaca tttgtggcag gtatgtcaat attgatgtca
     3301 ggtgttcgta tgttcttagc tgaaataact gcagcatttg taatcatttc agaaaagtat
     3361 attccaggtt cacgtcctgc gctagatgtt ccaacagtat ttccatatgc accaaatgca
     3421 gttattattg ggtttatatc agcatatgct gctggtctat tagcaattgc tattatggca
     3481 tcatttccta gtatatttcc tgtagtaatt attccagctg ctcacatatg tttcttctca
     3541 ggaggtacag ctgcaatatt tggcaatact tcaggaggat ggagaggggc tattgctggg
     3601 tcatttgttg ttgggttatt acttgcattt ttaccagtta tactatatcc agtttatggg
     3661 actcttggta tagaaggagc aactttccct aatattgatt acaacgtagt tggttcgata
     3721 ttacataata ttttacaatt tataaaacct atttaaacta aattatataa aagtatagtc
     3781 atagttacta tgatttcaag gaggatacat cttatggaaa atacattatc tgaattttta
     3841 gataatatat ctaatgaatt gtcagaattt attgcctcaa tagatgagac atctataaat
     3901 aaagcatgcg aattaattct tgaagctgaa aaaaatcacg gacgtgtaca tgtgactggt
     3961 attggtaaac caggacatgt atcaggatat atttcatcat tgctttcatc tactggaaca
     4021 tcagcatata tattgcatgg tacagaagca gtacatgggt caagtggtca agtagttgaa
     4081 ggagatgttg ttattgcaat atcaaatagt ggtgaaactc aagagttaaa agcaactctt
     4141 aagactctaa aagtaaatgg agcaaaaata attggtgtaa gtggtaatga gagttcattt
     4201 ttaaaaaata tttctgatat attcctgttc gcaggcgtta aacaggaagg agattgtctt
     4261 aataaagctc ctcgtgcttc aattttagct gaaacaatag tattacaatc attaagtgtt
     4321 gtattacaat atgctaaagg tcttaataca cagcagtatt tgaaatggca tccagcagga
     4381 agtcttggaa agtctattag agagggaata taattttatg aaatataaag ggattatatg
     4441 tgcaatgata acaccatttg atgaaaagca aaatattaat tcacaagcaa catgtaaatt
     4501 aattgattat ttgattgaaa aggggattta tggattattt attttgggta ctaatggtga
     4561 atgtcatgtg ttaacagatg atgaaaaagt tgaatttgca aagattgtta ttaatcatac
     4621 aaataatcgt gtacctgttt ttgttggaac aggtggtaat tcaactcgtg aagttataga
     4681 tttatccaag agaatggaag cgattggagc aagtgcatta tctattatta caccatactt
     4741 tgttacacca acacaacagg aattaatctt acattacaaa actattgcta attcagttaa
     4801 tttacctatt ataatgtata atatgccagg taagactgga attaatattg aaccagagtc
     4861 agtacgtgaa ttatcaaaga taaagaatat tgttggaatt aaggatagta gtggtaaact
     4921 tgacaacatg aaagcttata ttgaagtaac gcagaatgag aattttagtg tgttttcagg
     4981 ctcagattca cttattctgg atagtctaaa agctggtagt caaggagctg ttgcagcaac
     5041 aacaaacttc ctcacagaga ttggtatttc tatctatgaa aactttatga aaggggattt
     5101 ggaagaagct caaaaagctc aaagtagtat agaagaactt cgacgtatac taaaacttgg
     5161 aacaattcca tctgttatga agaaaactat tgtcttgaat ggaattaatg ttggaactgc
     5221 acgacttcct gtaactgaac caactgggga agtacttgaa gaaattaaac gagtagttga
     5281 aaattatagg acaatactta ataaaagtag tgagaataga taatataaat agggaaatta
     5341 cactggtata taattaatat atatattata aaacatgact aaaattaatg aataagtaaa
     5401 gtggtttgat gagtatagag gtgataatat gaaaaagatg aatataacca aaagaatgtc
     5461 agaatgtggg gctcttgcaa ttgtaagaga agaaaatctt aaccgtgcat gtgaaattgc
     5521 tgaaggatgt ataaaaggtg gtataacagt tatagaaatg agttataccc taaataatgc
     5581 tggagaaata attcaagggc ttaataaaaa gtatggtgaa atactttgtg tgggagcagg
     5641 aacagttttg gatagtgaaa cagctcgtca tgctatatta catggagctc aatttattat
     5701 tgcaccaaat tacaatgaag gtgtagcgaa gatttgcaat agatatcaga ttccttatgc
     5761 tccaggatgt acatcattga ctgaggcagt agatgcacta agtttaggag cgacttttat
     5821 aaaagctttc ccaatatcag atttctatgg acctaaacta gtaaaggtat ttaagacacc
     5881 aatacctgat atgccagttt tagcaagtgg aggaattaat cttgaaaacc tacatatatg
     5941 gttagaaaat ggagttgatg tatgtggatt tggtggttta ttaactaaag gaactattga
     6001 ggatattact gaaaatgcca aaaagattcg tgaaatcatt aaaaatataa gagaaattta
     6061 acatggagga tgaattagtt ttctattgac gagatatcca cacattaatt cctaagttag
     6121 aagaaatatt ttctagttga ggtaaagttt atattgaact taaattaaat tttgttttaa
     6181 ataagatatt attttagttg attg
//
LOCUS       DAEPLF010000195         6161 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEPLF010000195 DAEPLF010000000
VERSION     DAEPLF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.196"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            88..546
                     /locus_tag="KR314_003012"
     CDS             88..546
                     /locus_tag="KR314_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329377.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            543..1127
                     /gene="clpP"
                     /locus_tag="KR314_003013"
     CDS             543..1127
                     /gene="clpP"
                     /locus_tag="KR314_003013"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBH0329378.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            1449..2525
                     /locus_tag="KR314_003014"
     CDS             1449..2525
                     /locus_tag="KR314_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0329379.1"
                     /translation="MIAISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            2592..4631
                     /gene="bclA3"
                     /locus_tag="KR314_003015"
     CDS             2592..4631
                     /gene="bclA3"
                     /locus_tag="KR314_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBH0329380.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGGTGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGGTGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGATGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGVTGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGPTGPTGA
                     SAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAF
                     SMPRDGTITSISAYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPL
                     TGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            4800..5561
                     /locus_tag="KR314_003016"
     CDS             4800..5561
                     /locus_tag="KR314_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBH0329381.1"
                     /translation="MKNKIETSMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            5870..6148
                     /locus_tag="KR314_003017"
     CDS             5870..6148
                     /locus_tag="KR314_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329382.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGEYIYIPRKSNNKKEWGSSTS
                     TREDLSIRNMQIYEDYQVGYDLKYLSEKYYLSLKSIQRIVLQEKRKDS"
BASE COUNT         2355 a          873 c         1298 g         1635 t
ORIGIN      
        1 agcaatttat attaagaaaa aactttttgc ataggttata atacttaaaa ggagaggata
       61 gagttgaaaa caaatacaga tgttattatg gagataattg attatataga agaaaatctt
      121 acagaaaatc taaatttaga taaaatagct tatattgcta attattcaaa ataccattta
      181 cacagaatgt ttacaaatgt tgtaggatgt actatccatc aatacataca gaggaggaga
      241 cttactgaag cagctagaca acttatattt acagacaaat atattataga tatagctatt
      301 acagtaggat attctacaca tcaatctttt acactagcat ttagaaattt atataaagaa
      361 tctccacaag tatatagaaa taaagatgaa ttttatccta ttcaacagag atttgtttta
      421 aatctaaatc atcaaattca aagcgaaaaa tgtatttctt atgcaaatat gaattataaa
      481 caaagaagag atgaaatcaa ttcttatctt aactttaata ttaattgtga ggtgaaagta
      541 gcatgagttt agtgccacat gtaattgaac aaacaggtca aggggaaagg tcttatgata
      601 tatattcaaa attattaaaa gatagaatta tatttatagg agaagaaatt aatgatgcta
      661 ttgcaagttt agtagtagct caattactgt ttttagaatc agaagaccca gattcagata
      721 taattatata tataaatagc cctggtggct ctgctacatc aggatttgct atatatgata
      781 ctatgaatta tgtcagatgt gatgtatcga ctatctgtat tggtatggct gcaagtatga
      841 gtgctttttt acttgcagga ggaacacatg gaaaaagatg tgctttaccc aattcagaga
      901 ttatgattca tcagccaatg ggaggagcta aaggtcaagc aacagatgta aaaatagctg
      961 tagataatat attgaaaata aaagaacgat tagataaaat tctttctgaa aatacaggta
     1021 aatcaataga agatattaga agagatacag atagagataa ttttatgact gcactagagg
     1081 ctaaagagta cgggctgatt gattatataa tgaataggaa tgaataggaa tgaataaagt
     1141 atcaaagtat catattaaaa tgtctgtttt acaaatatat atataatatt aaataagagt
     1201 gtctcaaaat gaacttttta gtttatggga cactcttttt tgtatgaaat taaatatggt
     1261 tttatcattt caacaattat taccaaattt caagtttaat aaattctact ataaaaaata
     1321 cataaaagat aaggttcttt agtaactttc tgtcgtcaaa agtttttata aatttaataa
     1381 caaaacaaga gaaaatacca tatattactt atgtctgcaa tatatagaag acaaaggagt
     1441 tgtacattat gattgcaata agtttgtgca tgattgttaa gaatgaggaa gaagttatag
     1501 gcagatgctt agaatgtgta aaggatatag tagacgaaat tataattgta gataccggtt
     1561 ctacagattc cacaaaagaa atagtctcaa aatatacaga catggtgtac gatttcgaat
     1621 ggatagatga tttctctgct gcaagaaatt tttcattttc taaagcttct aaagactata
     1681 ctatgtggct tgatgcagat gacataattt tagaagctga tagagaaaaa ttattaaaga
     1741 ttaaagaaag cttggatact tccattgata ttgttatggc aaaatacaat gtatcctttg
     1801 atgaaaatgg taacccaact ctctcatatt atagagaacg attgtttaaa agaagttcta
     1861 atcataaatg ggttgaccct attcatgaag tagtacctct ttttggagag gtattttatt
     1921 ctgacattgc aatatctcat aaaaagcttc atagacagga ccctctaaga aatcttagaa
     1981 tttttgaaaa aatgatttca gaaggaaaaa cattggagcc aagacaccaa ttctattact
     2041 ctagagagtt gtactataat gctaggtata aagaagcaat agaaggtttt actaagtttt
     2101 tagactcatc aagaggatgg attgaggatt gtatcagtgc ctgcagagat ttagccacat
     2161 gttattactt aattaatgat gaaaaaagtg ccttatattc attgtttaga agtttcgaat
     2221 ttgatgagcc aagagcagaa atttgttgtg acataggaaa gcatatgttt gatagacaaa
     2281 aatataaaga agccattttt tggtataagg tagcattaac aagagataag aatgatacta
     2341 atggaggttt taagtcaaat gattgttatg gatatatacc atctatacaa ttaagtgtct
     2401 gttatgacag gttaggagaa tcagataaag ctatttatta tcatgaaaaa acaaaagaaa
     2461 tcaaacctaa cgatagtgct gttttgcaca atgaaaatta ttttagtaaa tttaaaaata
     2521 gttgataggt cacaatcata ctaaatttga catattatat atagagctta aaagctcaaa
     2581 tatatcagga ggtgctttta ataatgagta gaaataaata ttttggacca tttgatgata
     2641 atgattacaa caatggctat gataaatatg atgattgtaa taatggtcgt gatgattata
     2701 atagctgtga ttgccatcat tgctgtccac catcatgtgt aggtccaaca ggcccaatgg
     2761 gtccaagagg tagaaccggc ccaacaggac caacgggtcc aacaggtcca ggagtagggg
     2821 gaacaggccc aacaggacca accggtccga ctggcccaac aggaaataca gggaatacag
     2881 gagcaacagg attaagaggt ccaacaggag caacaggggg aacaggccca acaggagcga
     2941 caggagctat agggtttgga gtaacaggcc caacaggccc aacaggagcg acaggagcaa
     3001 caggagcaga tggagtaaca ggtccaacag gtccaacggg agcaacagga gcagatggaa
     3061 taacaggtcc aacaggagca acaggggcaa caggatttgg agtaacaggt ccaacaggcc
     3121 caacaggagc aacaggagta ggagtaacag gagcaacagg attaataggt ccaacaggag
     3181 cgacaggaac acctggagca acaggtccaa caggggcaat aggagcaaca ggaataggaa
     3241 taacaggtcc aacaggagca acaggagcaa caggggcaga tggagcaaca ggagtaacag
     3301 gcccaacagg cccaacaggg gcaacaggag cagatggagt aacaggccca acaggagcaa
     3361 caggagcaac aggaatagga ataacaggcc caacaggggc aacaggagca acaggaatag
     3421 gaataacagg agcaacaggg ttaataggtc caaccggagc aaccggagca accggagcaa
     3481 caggcccaac aggagtaaca ggggcaacag gagcagcagg actaatagga ccaaccgggg
     3541 caacaggagt aaccggagca gatggagcaa caggagcgac aggggcaacc ggagcaacag
     3601 gtccaacagg agcagatgga ttagtaggtc caacaggagc aacaggggca acaggagcag
     3661 atggattagt aggcccaaca ggtccaacag gggcaaccgg agtaggaata actggagcaa
     3721 ccggagcaac aggagcgaca ggtccaacag gagcagatgg attagtaggt ccaaccggag
     3781 cgacgggagc aacaggagca gatggagtag caggtccaac cggagcaaca ggggcaacag
     3841 gaaatacagg agcagatgga gcaacaggtc caacaggggc aacaggtcca acaggagcag
     3901 acggattagt aggtccaaca ggagcaaccg gagcaacagg attagcagga gcaaccggag
     3961 caacaggccc aataggagca acaggtccaa caggagcaga tggagcaaca ggggcaaccg
     4021 gagcaacagg tccaacaggg gcagatggat tagtaggtcc aaccggagca acgggagcaa
     4081 caggggcaac aggtccaaca ggcccaacag gtgctagtgc aataatacct tttgcatcag
     4141 gtataccact atcacttaca actatagctg gaggattagt aggtacacca ggttttgttg
     4201 gctttggtag ttcagctcca ggattaagta tagttggtgg agtaatagac cttacaaacg
     4261 cagcagggac attgactaac tttgcatttt caatgccaag agatggaaca ataacatcta
     4321 tttcagcata cttcagtaca acagcagcac tttcacttgt tggttcaaca attacaatta
     4381 cagcaacact ttaccaatct actgcaccaa ataactcatt tacagctgta ccaggagcga
     4441 cagttacact agctccacca cttacaggta tattatcagt tggttcaatt tctagtggaa
     4501 ttgtaacagg attaaatata gcagcaacag cagaaactcg attcttacta gtatttactg
     4561 caacagcttc aggtctttca ttagttaata ctgtagcagg atatgcaagt gcaggaattg
     4621 caataaatta gattagataa gaaaaggctt ctaatatcgt attgatatta ggagcttttt
     4681 taagaatgat tgatgaacat agttttaata ttaattagtg cgcatattat aaaatactat
     4741 tatataatat aaaataataa cattaaattg gaaatataga attatctagg ggtgatacta
     4801 tgaaaaataa gattgaaaca tcaatgaaca actttgtaaa aaattatcat aaatcaaata
     4861 atataactac aaagtggcaa gaacttttgt ttgcttatgc atctgctgaa gatgatatgt
     4921 ttcagaagct aaaaagtgtt gtaggaacat ctcatgctat gccaaaagat tttctggaaa
     4981 atggaaaaac tataattaca tattttatac cttttgatga aagtgtaaat aatagtaata
     5041 ttggagggtt tgaatgttct gaaatatggg caagagcgta catagaaact aatatgttga
     5101 tttcaaattt aaatgatttc ttatgtgaag aactagagaa gatgggggct aattcatatg
     5161 ctattcctgg gtcgtataat tttgacagtg aaaaattggt aagtgactgg tcacaaagac
     5221 atgtagcatt catagcaggt cttggaacat ttggtttaaa taatatgctt attacagaaa
     5281 aagggtgttg tggaagaata ggaagcataa tcacaagttt agaattagag ccaactaaaa
     5341 gaccagaaaa tgaatactgc ttgtataaac ataataatag ttgcaaaaaa tgtgtcaaca
     5401 aatgcgtgaa agaagcttta aaagaagatg gatttaatag gaataagtgt tatgaaatgt
     5461 gtatgataaa tgaaaaaaaa tacacttata taggtggttg tgatatttgt ggaaagtgta
     5521 tagttggggt tccttgttcg gtaataaatc cctcaagata aaggacttat gttgattaat
     5581 aactttaaat atatctaata agaagaaaaa agttagtctc taaaatagat gaattatgaa
     5641 aataaataaa gataatataa aggttagatg aagaagttat gttaattgac aactatttat
     5701 actgctgata gacttatatg tatcgttgca atatataagg aggggtgaga tttatgtttg
     5761 atttaatctt tatttttgca tagcaagggc tattgcaaat ataaaattta atatataaat
     5821 agccctttgc tattcctaat aaaaaattat tatttaatag gagtgcagaa tgagctataa
     5881 aaaagcaata catatattac cagaagaatt acttgaattg atacaggaat atgtagatgg
     5941 agaatatatc tatattccta gaaagtctaa taataaaaaa gaatggggaa gtagtacgtc
     6001 tactcgtgaa gatttatcaa ttagaaatat gcaaatttat gaagattatc aagttggata
     6061 tgatttgaaa tatttatcag aaaaatatta cttgtctttg aaaagtattc aaagaattgt
     6121 attacaagaa aaaagaaaag atagttaaga atgagccagt a
//
LOCUS       DAEPLF010000196         6074 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEPLF010000196 DAEPLF010000000
VERSION     DAEPLF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.197"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(54..662)
                     /locus_tag="KR314_003018"
     CDS             complement(54..662)
                     /locus_tag="KR314_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBH0329383.1"
                     /translation="MVDNLIKYIHKSNIAIVVLHEIYGVNNFIKDVCQKYYKGGYDVF
                     CPELLGEKRVFSYLKAQEAYDFFINNVKFDIYKKVEKLINGLKSEYKHVFVVGYSIGA
                     TIAWRCSENSLCDGIICCYGSRIRDYMDVIPTCPILLVFAKYDSFDVDYVSSQLSRRQ
                     NIQIEILDANHGFIDTYSKNYSNAQTEVFNIKEKNFLSKCLG"
     gene            complement(782..1210)
                     /locus_tag="KR314_003019"
     CDS             complement(782..1210)
                     /locus_tag="KR314_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0329384.1"
                     /translation="MYGVIGATVLVIFVILTAGIMQGKKVDFQLPTVFMIVTFIVSAI
                     SEEIVFRGYIQTRLTGLIKNSVLSSCINAFLFLSTHYPVKWISSGDFSFAILSGFYVI
                     CLILLNFSCDLVYRKTNCLWGAWLLHILYNIGTGVLIFTT"
     gene            complement(1229..1489)
                     /locus_tag="KR314_003020"
     CDS             complement(1229..1489)
                     /locus_tag="KR314_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329385.1"
                     /translation="MFKTVFEDKEYVLLCKNYSKSDGVKAIFIYLISMLLLFFQGYLY
                     TTNLSSTILTNSQMILSILILLIGLGFVLLSKEKLNTIGITM"
     gene            complement(1763..2308)
                     /locus_tag="KR314_003021"
     CDS             complement(1763..2308)
                     /locus_tag="KR314_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBH0329386.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(2426..4249)
                     /locus_tag="KR314_003022"
     CDS             complement(2426..4249)
                     /locus_tag="KR314_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329387.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGVLASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(4262..6037)
                     /locus_tag="KR314_003023"
     CDS             complement(4262..6037)
                     /locus_tag="KR314_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329388.1"
                     /translation="MKYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLREKIGVVFQNSSVFGKSILENIVMGNEGISREDVIKSC
                     EIANANQFITKLENSYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         2087 a         1054 c          552 g         2381 t
ORIGIN      
        1 gtcattatgt ttttcaaatt gattaaattt tctatatttt ataataaaac atattagcct
       61 aaacatttag atagaaagtt tttttcttta atattaaaaa cttctgtttg agcattagaa
      121 tagttttttg agtatgtatc aataaaacca tgatttgcgt ctaaaatttc aatttgaata
      181 ttttgccttc tagaaagttg agaagaaaca taatcaacat cgaatgaatc gtattttgca
      241 aacactagca aaataggaca tgttggaatt acatccatat aatctctaat tcgggagcca
      301 taacaacaaa ttataccatc acacaaagag ttttcactac atctccatgc aatagttgca
      361 cctatactat acccaactac aaatacatgt ttatactccg attttaaacc atttataagt
      421 ttttctactt ttttataaat atcaaactta acattattta taaaaaaatc atatgcttct
      481 tgagctttta agtatgaaaa aactcttttt tctcctaaga gttctggaca aaacacatca
      541 tacccacctt tataatactt ttgacaaaca tcttttataa aattattaac tccataaatt
      601 tcatgcagta caacaatagc tatattactt ttatgtatat acttaatcag attgtccacc
      661 acctgtcgat attttttaca tctagtttaa attattttaa gtatgtcaat ttactagctc
      721 taaaatttga cttataaaat aatccgtttc ttacgataaa ctttctgcaa agtaaaattg
      781 actaagtagt aaaaatcagc actcctgtgc caatgttata caagatatgc agtagccatg
      841 ccccccacag acagttagtt tttcgatata ctaaatcaca tgaaaagtta agtaatataa
      901 ggcatatcac ataaaatcca gatagaattg caaatgaaaa atctccactt gaaatccatt
      961 ttacagggta atgtgtagat agaaaaagaa aagcattaat acaagatgaa agtacactat
     1021 tttttattaa tccagttaac cttgtttgaa tataccctct aaaaacaatt tcttctgaaa
     1081 tagcagaaac aataaatgta acaatcataa aaacagtagg taattgaaaa tcaacttttt
     1141 ttccttgcat aattccagct gttaaaataa cgaaaattac gagtacagta gcaccaataa
     1201 ctccatacaa aagactttta tttaaatttc acattgtaat cccaatcgta tttagttttt
     1261 ccttcgaaag tagtacaaat cccagcccta taagtaaaat caagattgac aatatcattt
     1321 gtgagttagt taaaattgtt gaacttaaat ttgttgtata caaataacct tggaaaaaca
     1381 atagtaacat agatatcaaa taaataaata ttgcttttac tccatcactt ttagaataat
     1441 tcttacataa taaaacatat tctttatctt caaatacagt tttaaacact ataaatactc
     1501 ctttctaatt aaattcttat ttatttctca tattttatca taaatatatg tttaaatcaa
     1561 attaattcta tttattacat aaatcagaga taataaaact aaaaaattaa tttcattaaa
     1621 actatattta taatgattta aatatttaca agattatagc tataaaaaat ataaaataca
     1681 actattaaaa aggagcatat cattataatt ttaaaaatca taaatgatat actcctaaaa
     1741 atctacttaa cttatattaa atttattttc caaaatatct atttaataat cctaagaatg
     1801 ctttaccatg tctagcttca tctttacaca tttcatgtac tgtatcgtgt atagcatcta
     1861 gtcctaattc tttagctttt ttagctaatt ttaattttcc atcagttgca ccatactcag
     1921 catcaactct aactcttaag ttttcttttg tatctgcaac tacaacttct ccaagaagtt
     1981 ctgcaaattt agcagcatgt tcagcttctt caagagctat tctcttataa gcttctccta
     2041 cttctggata accttctcta tcagcttgtc tactcattgc taaatacatt cctacttctg
     2101 tacactcacc aacaaagtta gctcttaatc cttcgattat ttcttcatct acaccttgag
     2161 ctactcctat tctgtgctca tcagcccaat ttttttcgcc tttcatttct tcaaatttat
     2221 cagctccaac tttacatact ggacattgtg ctggtgcagc atctccttca tgtatatatc
     2281 cacatactgt acaaacaaat ttcttcataa ttactttcct cctaaatttt atatatcaaa
     2341 ttaaattttt tatttattaa gttagtctat atatacccgc accatatacc tttaaacaat
     2401 ttttttaaaa gttttttcta aatacttatt ttccctgtgt caataatatg tttctataat
     2461 aatgtccctt taattttatc aattctttat gatttcctga ttccacaatt ctaccatttt
     2521 ctaaaactat tatattatca caatttgata tggtacttaa tctatgagct ataattatac
     2581 ttgtctttcc ataagttaaa ttatttattg cttcttgaat tttagcttct gttgctatat
     2641 ctatgttact tgtagcctca tccaaaataa gtattggagc attttttaat actgctctag
     2701 caatacttat taattgtttt tgaccttcac ttaaatcttc acccctatcc gataatacag
     2761 tactatatcc ttcacgcatt ttaactataa atttatgtgc atttgctatt ttactagcat
     2821 atataacttc ttcatcagta gcctccagat ttccatacct tatattttcc ataactgtat
     2881 cattgaatat ataaccttct tgtggaacta ctccaaaaca agttctcaaa tagtttctgt
     2941 taatagagta tatatcctta ccatctaaaa atatacttcc ttcatctata tgataaaaat
     3001 ttgtaattag atttataatc gttgtttttc cacttcctgt ctctcctatg atggcatttg
     3061 atgtgccagc tttgattttt aggtttatat tatctaaaat tttctttttt gaatcataag
     3121 aaaaacttac atctttaagc tcaatttctc cctttacaaa atgagagttt aaattaccat
     3181 ctttattatc gttaatctca cttttaaaca tgttatgcac ttcttgctct tcttgttcat
     3241 ctataatttc aaaaactctt tcacatccag ctatggctga aagtaatgta ttaaatagat
     3301 ttgctaattc atttaaaggt cttgtgaatt gtcttacata acttaaaaaa ctagctataa
     3361 cccctatggt tataatatct ttagatgcaa gaacacctcc tataatacat atagttgcaa
     3421 atcctatatt actaatcaca ttcataatag gcattaaaag agaagtgtaa atttgcgatt
     3481 taagggaaat atttagaagt ctatcattga cttctttaaa atcatttaca gattttcttt
     3541 cataattaaa agattttact acatttatgt ttgatatcat ttcttcaata tgtgcattta
     3601 gttttcccaa ctcaacttgt tgttgcttaa aaaaaactct agttctcttt gcaataaaat
     3661 tactcacaaa cattactatc ggtattaata ttacactgca aaatgttaaa attgggctta
     3721 atacaaacat cataataaaa gtcatcaata tgtttaagat tcctgtaatt aactgtacta
     3781 atgaatttga tattcctgta ctgatattat caacatcatt tgtcactcta ctcataatat
     3841 ctccagtaga acttttatca aaaaaggtta ttggtagatt tttaaatttt aaaaataaat
     3901 tacttctcat ttctttaact atactttgag ataatctagc tgtacatatc cccttagaca
     3961 gtttaactga aaactcaatt atataaaatg aaagtaaaat aattattcct tttaagaaaa
     4021 tggattttga ataagtatcg atatctatta tgtcaactac ctttccaata gtatatggta
     4081 ttacagttga tataattgaa tcaattatta ctagcaaaaa aataaatcta gtaatcttct
     4141 tatctttttt aaagtacata aaaattcttt taaacgtttt tttaaaatct ttaggctttt
     4201 cttttttctt taatctactg accttttgat ttccagtact taagttcaat atacttcctc
     4261 cttaatttac atcttcactt actaaattct gcaacgcaaa aattttttta taaattggag
     4321 aactaaccag taaatcttca tgtgtagcaa atccaaccac ctcaccctca tccaagacta
     4381 aaatcttatc acagtcaatt atagaactaa tcttttgtga aatagttatt acagtacatt
     4441 tcttactttc cttttctctt aaattactta tatactcttt tatattcttt cttataattg
     4501 tctctgtatt aatatccatt gcactaaaac aatcatcaag aattaatatt tgtggttttt
     4561 tggctatagc tctagctata gtaattcttt gtctctgtcc tcctgataaa tttttacccc
     4621 tttgaaataa aacctcatca taagaatttt cgagtttagt tataaattga tttgcatttg
     4681 ctatctcaca tgattttata acatcttctc tacttatacc ctcatttccc ataactatat
     4741 tttctagtat actttttcca aacacactgc tattttgaaa tactacacct attttttccc
     4801 ttaaactttc ttcagaaata ctgtatatac tacgtcctcc aatcttaata tcacctttat
     4861 caattatata taatttttga attaaattta ctaaagaagt ctttcctgaa ccagtttgac
     4921 caatcacacc aatattttga ccttcttcta ttttaaagga tacgtttttt aatatttttt
     4981 catctgtatt tttgttatac gaaaaatcta cattttcaaa aattatatca caatttacaa
     5041 actttaaatc tttttctcca acatctaaac tttcttctct ctcaattact tcttcattct
     5101 catcaaccaa aacctcactt attctctcat aagatgcttt tgccctaaca aaaacattaa
     5161 aaacatgtga aatcattata agagatgtta atattctagt catatagttt acataagcaa
     5221 ctataatccc aattttaatt tctcctgaat aaattaaatg ccttccaatc agtaatatta
     5281 ttattatgga aacattaatg attattgtaa ttgtcggtct aaaaaatgac atcatagttg
     5341 tagcttttct actattttta aaaagttcat catttaactc ttcaaagctt tggttctcat
     5401 aatcgtatct acaaaatagt ttaactactt ttattccact taaaaactgt cttaattttg
     5461 aatttacttt atctgtactt tcttgtacat ttttaaataa tctataccct atcttcatat
     5521 taagcaatat tataataaat ataattaaaa ctgcaattag aataattatt gacatcttta
     5581 aatttaattt tatagctata attaaacttc ctatacacat tattggtgac tttataaaaa
     5641 ctctcatcat tccattaacg aattgttgca tttgattgat atcattagta attcttgtta
     5701 ttaacgaagc tctaccaaat ttatctattt gttttaatga gtatgtattt atcttttcaa
     5761 atacatcctg tcttaaatca taagaaaaat gctgagaaac tcttgccgag attacacttc
     5821 ttattattgc aaatattgca cctaaaaaag ttatacctat cattatagct cctgttgata
     5881 gtatgtagtc taggttctta tttgaaaccc ctatatctat tatttttgcc attatagttg
     5941 gttgagctaa atcacaaaaa gcttccaatg ttaaaaaaaa tactgctgtt agaaataata
     6001 ctatatattt ttttatgtat tttttaaaat acttcattca atcatccctt catagaattt
     6061 tctcattacc aaaa
//
LOCUS       DAEPLF010000197         6011 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEPLF010000197 DAEPLF010000000
VERSION     DAEPLF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.198"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..68)
                     /locus_tag="KR314_003024"
     CDS             complement(<1..68)
                     /locus_tag="KR314_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0329389.1"
                     /translation="MAVVEKAYKFRMYPNKKQQELI"
     gene            complement(70..177)
                     /locus_tag="KR314_003025"
     CDS             complement(70..177)
                     /locus_tag="KR314_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0329390.1"
                     /translation="MGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            complement(814..2394)
                     /locus_tag="KR314_003026"
     CDS             complement(814..2394)
                     /locus_tag="KR314_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBH0329391.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            3192..4093
                     /locus_tag="KR314_003027"
                     /pseudo
     CDS             3192..4093
                     /locus_tag="KR314_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            4080..4835
                     /locus_tag="KR314_003028"
     CDS             4080..4835
                     /locus_tag="KR314_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0329392.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTSPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            4966..5988
                     /locus_tag="KR314_003029"
     CDS             4966..5988
                     /locus_tag="KR314_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0329393.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRIGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
BASE COUNT         2049 a          959 c          781 g         2222 t
ORIGIN      
        1 ttaattaact cttgctgttt tttatttgga tacattctaa acttataagc tttttccact
       61 actgccacct cactttactt gctttttctg attattaata tattttctta tctgctcctc
      121 tgtattttct gatacagtgg caacgaaata actaggattc caaagatgtc tccccataat
      181 actggatgtc tatatttgac atattatatg catatcctct accatgagta acttccattt
      241 ttcatcacct atcataatat taaaatatgc aattacttaa aacaagtatt ttgaaggttt
      301 tttatattta tttttaaata acatatttaa gaaccgtaag tcttaacttt tatcattttt
      361 gaggttgtcg ttcacataag ttcgctacta cctatgcagt tctcttatga acttcttaca
      421 ctttcatgta agcacagact atatcttatc cttcggcatt atccgctaag gtctatccac
      481 ttccactacc gataacttgt agtgtacttc cctcaagagg aatagtcgtt gaagtttctc
      541 ctattcggag cttacctgct gattgcccat tttaaagtac ttaggattta accttatacc
      601 atctaaccaa ttttttctac tttcgtaact ttcacactta ggtttatttc atccttatgt
      661 tttagtttag ttagctttag gggattttag caattcaagt agtattggat aggttctatc
      721 ctatctctac atataggttt ccctatatgc tgactatttt agctactaac tcatgactaa
      781 agtcacaagt gtgcgtagct attttttaat caattatata tccaacaact ctttcattgt
      841 taatgaaatg ttacttatta aatccgtagc atttaccgta tacattgctt tgctcaattt
      901 atctcctata tagccatgta tatatgctcc actaactgta gcttccaata tatccatacc
      961 ttgacctata aatgaagtaa tcattccaag caaacaatca cccattccac cagttgccat
     1021 acagctattt cctgtaggat ttatatatgt ttttttacca tctgttatga cagtttgata
     1081 accttttaaa agcaccacca gattatattt ttgagcaaat tcttttgcaa catctactct
     1141 atgttctctt atatacccaa ttgaatctcc tgtcaacctg gccatttctc ccaaatgagg
     1201 tgtaattaca aatctatttt tccattctaa aaacttataa catctgtcct tcataacgtt
     1261 caatccatct gcatctaata ctattgggca attagaattt tctactatct taagtaattt
     1321 atcaaatgtt tgactattat ctcccatacc tggaccaaaa ccaattgcat cactagaatt
     1381 aagtaattta tttaatcttt cttcttcaaa atttaaagtc attgcttctg taagtctaat
     1441 agataccttt tcttgcacat cttcactact aattaatgtt acaagtccac tcccactctt
     1501 aacagcagat tcagttgcta tgaaacttgc accataaaat ccttttgaac ctgcaactat
     1561 agatactttt ccaaaatcac ttttaaaacc atgcttatct cttcttctaa tattattttt
     1621 tacaaaagtc ttatcagtta tgaactcttc atcatggtat tttcttgtaa taaaatctgg
     1681 aacacctatc ttttctacaa ctacatcacc tataagtgtg ctagtgtcat agtttagaaa
     1741 acctctttta taaaactcaa agctaatcgt tttgtttgct ctaatacaag ttcctaatac
     1801 atttccatta tcgctatcta tacctgaagg aatatctata gaatatgtat ttttgctatt
     1861 atcgtttact atgtcaataa cttctttaaa aataccagtt atatcacgtt ttaatccagt
     1921 cccaaatata gcatctacta caacatcact tttagatact ctatctttaa aaattgttaa
     1981 atttttattt tttataatat tttgtttctc aactttaata attttatcta cttcattttc
     2041 atctccataa tttttttcac ttaaatcatt tttattaata tttataatat taattcccat
     2101 atttaaaagt acattgtaat ttattttaga gcattcactt agtttttcaa gattacctac
     2161 taaaaatacg tttacttctt ttccatatgt atttagatgt cttgctattc caagaccatc
     2221 tcctccatta tttccaacac cacatacaac tgtataacta ttatacatgt ctatatccat
     2281 atttttcatt gcagatataa ccgcattttc catcaataca atcattggaa tccctacttc
     2341 ttctacacat gcagtatcaa catttctgct taatttagat gtacctatta gcataaatta
     2401 tcactcctat aaagttaaag acttcttaaa tacttaccaa aaatatctat tgaaatcttt
     2461 taatcccata aatatcataa ataaaatcac taaaactaag agttttgaaa taatttaaaa
     2521 taacaaaaaa ttttgaatta aaacaattat ttaatttatt ataccatata atttaagtta
     2581 ttatgttgat ttaaaaccta tttacactaa ttatctaaat atctctttat ttttataatc
     2641 ttttatgact tatttatatt tttcattttt ataaaaaaac aataattaag aatataacta
     2701 tattctacat gtaaaaattt ttttatttaa actcaatttt ctgataactt gataattttt
     2761 caaaatttat agttgacttt tattttttac tgtattaaac taattactat acaaaacaga
     2821 aaacaaaatt ttcaaaatta ttaaattaaa aggagaggta aatatgaaga ctttattaaa
     2881 caaacaagta cgatttttta gtagccaatt tagttagggt ttgtacctaa gttaagacaa
     2941 attgtcataa catagataca agccccttag gattatgatt tttaaggatt gtatctatgt
     3001 tggctatagt aattgttgtt aaagttttta ttttatcatc ataatataga gccacatgga
     3061 ttatactgtg tggcttattc gaatttttgc catacagtta tatatactgt gtggtttttt
     3121 tatatcaaaa tcatttttta aaaccaaatt taaaaaacca tttaaaatat tttcaaaggg
     3181 gtgttttatt tatgtcaggt attatatctg taatgacgca aagtttaatt ttatcgatta
     3241 tggcactggg ggtttatata acttacaaaa tcctagattt tccagacatg tctgctgatg
     3301 gtagctatac aatgggtgct tctattgtag cattttctct tactaatggt atttctcctg
     3361 tagtagcaac tttaatggct atcttatgtg gttgtactgc tggtcttgtt acagggattc
     3421 ttcacattaa gtttaagata tctaacttac tttctggtat tttagttatg ggaatgctat
     3481 actctatcaa tttaagaata atgggaaaat caaatattcc tctatttagc tttaaacact
     3541 tattcaatgg ggaaatatct cctatagttt tagccttagc ttttgtattc atctgtaaag
     3601 tccttttaga tttattccta aaaacaggtc ttggatacac tttaaaaggt gttggtgata
     3661 actctcaaat gattaaatct ctgggtataa atataggttc tataaaaata ctaggactta
     3721 tgatttcaaa cggacttata gctttatctg gtagtcttat ggctcagttc ttgggattct
     3781 cagatgtaaa tatgggtata ggaactttag ttcttggaat tgcctctatc ataattggta
     3841 ttacactatt taagaaattt acttttatca aagatactac tgcaataatt gttggctctt
     3901 ttatatacca attcactata tactttgcaa tgagtttagg aatgttatca actgacttaa
     3961 aactaataac tgccatagtt ataatagctt tcctagctac tggtaactta aatatttcac
     4021 taaaaaaaac aaatgcaaag ctagtaccaa aaataaatca gaaaaaagag gtgttgtaga
     4081 tgttacaaat taaaaatctt tcaaagagtt tccctaatcc atatggtgaa cctaatacta
     4141 tctttgaaaa tttatcaatc gatatagaag atggtgaatt tgtaagtatc ataggtagta
     4201 atggaactgg taaatctact ttattaaata taatatccgg gcttataaaa gaatcttctg
     4261 gtcaagtatt attaaataaa cataacttgt ctaatttagc tgaacacaga agaactcaaa
     4321 tagtaagtag agtttttcaa gacccaagtc ttggaacttg cccttctatg actgttagag
     4381 aaaacttgtc tttagctctt aacaaaggaa aactattaaa tctaagaaaa tgtcttcgtt
     4441 acaaaagaga tttcttagaa aatttattgg aaggtgtatc tcttgattta aaaaaatacc
     4501 ttgatgttca agtaaaattt ttatcaggag gtcaaagaca atcattatca ctaattatgt
     4561 cgtgcttaac aagtccatca gttttattac ttgatgaaca tacagcagca cttgacccaa
     4621 aaacatcaaa tgaagtaatt gaattgacta ataagattgt aagggaaaaa aatataacta
     4681 cacttatggt tactcacaac ttaaaacacg ctcttcatta tggtgataga ttgataatgc
     4741 ttcataaggg tgaagttgta ttagatgtaa aaggtaaaga aaaagaacaa ttaacagttg
     4801 aagaaatctt agaaaaattt gaatatgcag tttaaaataa tatatctatt gtattaaact
     4861 cggctttaaa ataaagttaa aattacaata attatggtta ctttgtaata attcatatga
     4921 atatatgaac atatataata ataaattttt gagggggata taaaaatgat taataaaaaa
     4981 agattggcaa gtttgatttt agctggtgct cttagtatat ctatgcttac agggtgttca
     5041 caaggaggag actctggtaa ttcaaaacaa gaaagtaatt ctaaaggtaa agaagttaaa
     5101 aaaattggta taactcaact ggttgaacat ccagcattag atgccactag gataggtttt
     5161 gtaaaagcat tagaaaagaa cggatttaaa gatggagaaa atattgatat agacttccaa
     5221 aatgctcaaa atgacatgcc tactacacaa agtattgcga gtaaatttgc atctgataag
     5281 aaggacttaa tatttgctat atctactcca tcagctcagg cagcatttaa tgctactaaa
     5341 gatataccta tacttattac tgctgtgagt gaccctgttg ctgctggatt agttaaaact
     5401 cttgaaaagc caggcacaaa tgtatctgga acatcagatt ttgtttctgt agacaaaggt
     5461 ttagaacttc ttaaaatatt tgctcctaag gcaaaaacta taggagttat gtacaatact
     5521 agtgaagtaa attccaaagt tcaagttgat gctttaaaag aatatgcatc taaaaatgga
     5581 tttaaagttg ttgaaaaagg tataactact tcaaatgaag ttaaccaagg tatttctagt
     5641 ttagttggca aaatagacgt attatatgtt cctacagata acttagtggc ttcttctatg
     5701 cctatagtct ctaaaatagc tactgagaat aaaatacctg ttatagcagc tgaatctggg
     5761 ccagttgaaa aaggtgctct tgcttgtcaa ggtatcaact acgaaaaact aggttacaaa
     5821 acaggtgaaa tggcagttaa aattttaaat ggcgaatcag tatctgatat gcctgtggct
     5881 acatcagatg atacagatat cattgttaat gaagatatat taaaagcttt aggtatggaa
     5941 aaaccttcta atgaaaatat ttcttatgta aagactaaac aagaatagaa agatattttc
     6001 tatatacgat t
//
LOCUS       DAEPLF010000198         5997 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEPLF010000198 DAEPLF010000000
VERSION     DAEPLF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.199"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(17..598)
                     /locus_tag="KR314_003030"
     CDS             complement(17..598)
                     /locus_tag="KR314_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329394.1"
                     /translation="MLYLLKINQFERSYDIGIFKNEQSIYKFIEKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHYWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
     gene            935..1138
                     /locus_tag="KR314_003031"
     CDS             935..1138
                     /locus_tag="KR314_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0329395.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            1302..2456
                     /locus_tag="KR314_003032"
     CDS             1302..2456
                     /locus_tag="KR314_003032"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBH0329396.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELVNITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFRVEQENIDVVVHPQSIIHSMVQYADSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            2469..3473
                     /gene="rseP"
                     /locus_tag="KR314_003033"
     CDS             2469..3473
                     /gene="rseP"
                     /locus_tag="KR314_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBH0329397.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSNKVVNVTPKNNNGKYEIGIQPQREKDFLASIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            3656..4711
                     /gene="ispG"
                     /locus_tag="KR314_003034"
                     /gene_synonym="gcpE"
     CDS             3656..4711
                     /gene="ispG"
                     /locus_tag="KR314_003034"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBH0329398.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSRERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKHGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            5059..5883
                     /locus_tag="KR314_003035"
     CDS             5059..5883
                     /locus_tag="KR314_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329399.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLASTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSIWKNSAYENWLKSFQGKEVSNSVLENYYK"
BASE COUNT         2293 a          622 c         1068 g         2014 t
ORIGIN      
        1 tattttaaaa tataaattat ttaaaacgtt ttttgtactt atatagaaat tcttctatat
       61 tttttgattg ttctcttatt tcaactgctt ctatatctag taaataaagt atagaatcat
      121 ttgtaagttc aacatattca ccatcttctg aaccgagtgc atgtctctca atttttaatc
      181 ctttttcttc ataatatttt tttgtttcta tatataatga ttctcgcttt tgtatgtagt
      241 ctttcacttc attatttgga aagttatatg aatccactaa tgtttcgcca tttataaaag
      301 ttttctcggt agcaactttt tcatcccaat aatggatttc ttcccaagta aaaaatatct
      361 cagttccatc tggaatgaat gaaaacctgg taataatata taaataatta ttgaaattta
      421 cttcataata ttcaggaata tcttcaaatt ccatatagta atttgtataa ttaaaattac
      481 tattatcttt ttttacaaag ggtatttttt caataaattt atatattgat tgttcatttt
      541 taaaaattcc tatatcatag cttctttcaa attgattaat ttttaataaa tacaacattc
      601 tttctccttt tattcaaaat ataattttta taaatttact aatttttagc ttttttaatt
      661 atatatttac atatgtttat atgatactat aatgatgtgt tttaaataga tatataatag
      721 taaatatttt ttatatagtt atgttttagt aaaataatga gataaatata tataaagctt
      781 aatttttgaa cttatggttt tgttttattt taacatattt atatttaata aactataatt
      841 gaaatctatt tagataaata attatttatc taaatagatg aaaattttcc aaaatagata
      901 aaatgtgata gaatatattt tgcgaggtga taacatgatt tcatacgctc cattatggaa
      961 attgttaatt gacagaaaaa taaagaaaat ggaatttgta aatatatcag gtataagtat
     1021 ttctgtttta ggcagactag gaaatgataa atctgtttct atggatacaa tggagaagat
     1081 atgtttagca ctagactgta aaatagaaga tgttgtggaa ataaaaaagg gcatttaata
     1141 atactagaat attttataat attccacaag atacaaaaaa taatagatta attttaatct
     1201 gtggaataat atatgaaatt tctatgaata gtaaaaataa ttgttttagt agtgttataa
     1261 tgttttgaca gggtatattt attattagaa aggattatta tatgaaaaag atatcaattt
     1321 taggttctac aggctctata ggtaaacaaa ctttggatgt ggttagagaa catagagata
     1381 agtttgagat agttgcaata tcagctaaca gtaatattga attattgcta gaacaaatag
     1441 tagagtttaa acctaaatat gtaacagttt ttgaggaaaa taaggcattg aaattaaaag
     1501 aaatgttgcc taaaaatata gaaatagaag ttttagcagg aatggaagga ttaaaaataa
     1561 tatcatcact tgatgaagtg gatgtccttt taactgctgt agtaggtatg ataggattag
     1621 tcccaactct ttgtgctata aagaagggaa tagacatagc attagcgaat aaagaaacgt
     1681 tggtaactgc tggtgaactt gtaatgaaag aagcagaaaa atacaatgta aatattcttc
     1741 ctgttgatag tgaacatagt gctatatttc aatgtctaaa tggagaaaat aaaaaaaata
     1801 tagaaaagat aatacttaca gcatctggtg gtccatttag aggaaagaaa aagggagaac
     1861 ttgtaaatat aactaaaaat gaagctttga agcatccaaa ttggagtatg ggaagaaaaa
     1921 taagtattga ttcttcaacg cttatgaata aaggacttga agtgatagaa gctagatggt
     1981 tgtttagggt agaacaagaa aatatagatg tagtagttca cccacaaagc ataattcatt
     2041 caatggtgca gtatgcagat agctcaataa ttgctcaatt agggtgtcca gatatgagac
     2101 taccaataca gtatgctctt acatatccag atagaatgga aagtagcttt gaaagaatga
     2161 atttttcaaa atttagtact ttaacttttg aagaaccaga tttagaaact tttccatgtt
     2221 taaaattggc ctatgagtgt ttaaaaatgg gaggaactta ttcttctgtt ttaaattcag
     2281 caaatgaagt tttagtaagt gaatttttag aggataaaat tggtttttat gacataccat
     2341 attacataga gaagacttta gaagttcata gtagcataag cgaaccaaca ttagaagaaa
     2401 tcttagaaac agatagatgg agtagagctt atgttgcaaa tctgataaaa aaataggaag
     2461 gtgaatttat gactataata gccgcattaa tactatttag tataattgta ttaatacatg
     2521 agttaggaca ttttatattt gcaaaaagaa gtggtatcaa agtaaatgag ttttctatag
     2581 gtatggggcc taagatatat agtgtaaaaa aagatacaga gtattctata agagcacttc
     2641 caattggtgg atatgtcagc atggaaggtg aagatgaaga acaaataagt ccaaattctt
     2701 ttggaaacaa gtctatatta caaagattta gtactattgt agcaggacca atattcaata
     2761 taatattagc agcaatactt ttagttccag tatttttgta tataggttct ccaacgacta
     2821 aattaggaaa gataatgcca gatactcctg cacaggctgt agggcttcaa gtaggagata
     2881 agataaataa aattaatggt aattctgtta agacttggga tgaagttgca aatataataa
     2941 acacttcttc tggtggagaa ttaaaactta gtataactag agatggaagt aataaagttg
     3001 taaatgtaac acctaaaaat aacaatggaa agtatgaaat agggattcaa ccacaaagag
     3061 aaaaagactt tttggcgtca atagtaaatg catgtaaaac tactgtagat atgacaaaac
     3121 agatgttaac atttctaggt caaatgatta caggacgtgt accaggaggg attggcaatg
     3181 ctgtagcagg accagtaggt gttattggta tggtatcaga tgctgctcgt acaggcatca
     3241 taaatgtagt gtatttagca gctgtcataa gcttaaattt aggtatagta aatctattac
     3301 caataccagc acttgatgga tggagaatac ttatgctatt actagaagct gttagaggtg
     3361 gtaaaaaact cgacccaaac aaagaaggca tgataaatgt tgttggtttt ggagctttaa
     3421 tgttatttat gctttttatc acatataaag atatattaag attatttcaa tgatttagtt
     3481 ttttagagat tttaagaaaa taggctatgt agttttaaat aagaataaat taatttttat
     3541 gtatgaaatt tattcttatt taactagata gctttttaat ttataatata catgtaggtt
     3601 ataatatttt actatttaaa gagattcaat ataaaataat atatagaggt ggaatatgag
     3661 ttacgagaga agaaagacga gagaagtaag tgtaggaact gtaaaaatag gaggagaaaa
     3721 tcctataagt atacaatcta tgacaaatac tgatacaaga gatgcagatg ctacaatagc
     3781 tcaaataaag agactagagg aagctggttg tgatatagtt agagttgctg ttcctgatat
     3841 taaagctgct aagaatatag caaagataaa gtctagtgta aatataccaa ttattgcaga
     3901 tatacatttc gattataagc tagcccttga agctatagaa caaggtgtgg atggaataag
     3961 aataaatcct ggtaatattg gaagtagaga gagagttaag atggtagttg agaaatgtaa
     4021 ggaaagaaat ttaaaaatta gaattggagt caatggaggt tctttagaga aagaactttt
     4081 aaaaaagcat ggctctgcaa ctgctgaagc attagttgaa agtgcaatgg gacatattaa
     4141 gattcttgaa gacttagatt ttcataatat agttatatct ctaaaatcat ccgatattta
     4201 taaaactgtt gatgcatatg aattaatatc aaaaaaagtt gattatcctc ttcatattgg
     4261 aattacagaa tctggaagtg ttcacaaagg aacaataaag tcttctatag gggtaggagc
     4321 acttcttctt aaaggaattg gagatactgt tagaatatct ttaacaggcg accctgttga
     4381 agaggttatt gttggtaaac agattttaag aagtttggga cttttaaatg ataagattaa
     4441 agttatatca tgccctacat gtggtagatg taatatagac ttaattagtg ttgtaaatga
     4501 ggttgaagaa aagattggta gtatggaaaa aaatatcact gttgcaatta tgggctgtgc
     4561 tgtaaatgga cctggtgaag ccagagaagc agatataggt attgctgggg gtaaaggtga
     4621 aggtctttta tttaaaaagg gtgaaatagt tagaatgatt gatggtaaca aattagttga
     4681 tgagttgcta gaagagattg aaaaattata gtatacaatt catatatata ttaattgact
     4741 agatttttaa atacttttgt aataagtttt tatttacagt atttaattat ctagtcattt
     4801 tttattttat aaacttcatt aaatgtaaga aacttatatt ctaatttgac tatatatgta
     4861 tgagttaaat catattgtaa atttataaaa ttaaaaaaag atttaatata gtgaaaattt
     4921 ccatcaatag tattgaaact tgatagtaac tatgatagga tatagttact agaacagttg
     4981 gtaaattatg ttaacataga ttttaatgtt ttggtaaatt gttttattat aagttaatag
     5041 aaaaagaggt gaagaactgt gagatataaa aaaatatcta gcttacctat tttagttgtt
     5101 gctttgctga ttactggaac gacaacaaca ttttctctta gtacgaccaa ttcagaaaaa
     5161 acaaatgtat acttagcaag tacggtaagt actgtcacaa agaaagtatc cgatattcaa
     5221 agtagtaaag gtcttgttta taatgagaat attccattat ttataaataa tagcaaaata
     5281 cagtatgatg atactccata tcattggcct agcaatgtta taagcttaac aaatagcagt
     5341 gaaaaggcaa ttatggatta tgagattaca tgcttagcat atgataaaaa tggaaaacca
     5401 cttgagctat attgggatgc tcaaaatgtg gcagcagatg gcgaagtagg aagtgttgga
     5461 tttagcccag ctggtgtaga ctatggtatt gttacaggca tatctcctgt gtctccaaaa
     5521 tcatattcac atacttatag aaaaatgcaa caatcaccac cacaggatat aataagtatg
     5581 tttgaaaaac aacaaggcaa ggcatgggta gaaaattggt tgaaagaatg gaaacaaatg
     5641 gagaaagaat atgcaaagca aaatgcaata gcaccaggta agaatcaaaa tgatgccttt
     5701 ctattgtttg ataaatggaa acaatcaact ggagagcatg gtgtaaagta cattatatct
     5761 tgtgtaaaac aagtaacatt taatgatggt agtatatgga aaaattcagc atatgagaat
     5821 tggttgaaaa gttttcaagg gaaggaagtt tcaaatagtg tattagaaaa ttactataag
     5881 taatgtttaa tgatagcaaa atgagtacga atttaattaa tgttaattta attaaataag
     5941 gactattttg tttaaaaata tcagtgttac aaggaaattt ttattgtgac aaaatgg
//
LOCUS       DAEPLF010000199         5995 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEPLF010000199 DAEPLF010000000
VERSION     DAEPLF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.200"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            87..740
                     /locus_tag="KR314_003036"
     CDS             87..740
                     /locus_tag="KR314_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBH0329400.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNSFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDEIRGAIFDVSQAIDLTKLMGIIIK"
     gene            881..2152
                     /gene="brnQ"
                     /locus_tag="KR314_003037"
     CDS             881..2152
                     /gene="brnQ"
                     /locus_tag="KR314_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBH0329401.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAIFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2354..3541
                     /locus_tag="KR314_003038"
     CDS             2354..3541
                     /locus_tag="KR314_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBH0329402.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMTKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(3771..5795)
                     /locus_tag="KR314_003039"
     CDS             complement(3771..5795)
                     /locus_tag="KR314_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0329403.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGEVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKRAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
BASE COUNT         2111 a          710 c          935 g         2239 t
ORIGIN      
        1 ctttttaata tatcaaatat gtatattaag ttaatatatt aaaaaaaata atatcaaaaa
       61 taatataatt tatagtggag gggaaaatga ctaaaattat taaagtgcaa aaacctaaat
      121 ttacaggaga attagaaatt attgaaaatc ttgaagatat acttgaagat atatttaatg
      181 atgaaaaaat atttaataaa attatagaaa atgaagttat aagtgattta gattctgtaa
      241 gagttagctt tgaatcttgt atatttagaa atgtatcctt tgaaaattgc aatttaaaaa
      301 agattgattt acttgatgtg gtatttgaaa actgcaattt atcaaattca ttttttgatg
      361 gaggaagtat atacagagtt gaatttaaaa atagtaaatt gataggagtt agatttgatg
      421 attgtatact taaaaacgtt ttgttcaagg atgttttagg taagtattct aacttttcat
      481 tctctaaatt taagatagtt tctatcttag attcaaattt tgaaaattct gtatttcaag
      541 aagtaaaagg tgacaatcta gtatttgagt atacaaactt aaaaaaagca aactttaatg
      601 gaactaaact ttctggcata gattttacca ctaataatat tgagggaatt gagattggta
      661 tagatgagat tagaggagca atttttgatg tgtcacaagc aattgattta acgaaattga
      721 tgggaattat cataaaatga aaaaaatatt cataaaataa attgggaaaa cgttaacaat
      781 gtgtgaattt ttaccagatt gacatttttc tgaaaataaa gtaagattat cttaatattc
      841 aaaatatttt caaagggggt aatttagtgt gagtaagcag atgagagata tttttatagt
      901 tggttttgct ttattttcta tgttctttgg tgcaggaaat cttatgtttc ctccattttt
      961 agggatggaa tccggcaaag actggttaat tcctcttatt ggatttgttg ttgcagatgc
     1021 tggtatagca atgcttgtta ttatagcaac agcaagatgc aagggaagca tggatgatgt
     1081 acttaggaga gctggtaaag ggttagcaag agtaatgagt gtagctgctc tagcatgttt
     1141 agcacttctt gtaataccaa gaacttgtgc aacaacatat gaaatgggta ttatgcctat
     1201 atttggagac catggaccag tagctaaagc tatattttca ataatcttct ttgggttaac
     1261 tttagcattt actattaggt cttctaaggt tatagacata ataggtaagt atttaactcc
     1321 agcgttatta ttagtattat ttgttttaat agtaaagggt ataatttctc cagcaggacc
     1381 tatgagtcct gagcatatga tagataaaaa tctatttggt gaaggtatat cacaaggtta
     1441 tcaaacaatg gacgctctag gtgcagcatc tatggcaact ataatgttga tgtctataat
     1501 agctaaagga tatacatcag ataaagacca aataggaatg actattaaag ctggttttgt
     1561 agcctgtgta tttctagcag tagtatatgg tggtcttact tatttaggtg caacagtttc
     1621 aactttatat gatactagta tacctcaagc atctttatta gttgaaataa ctagtatatt
     1681 gttatatggt caagtaggaa aggtaatttt aggtgtaatt gtggcacttg cctgtctgac
     1741 tacttctaca ggtttaacag catcaatatc aacttatttt gaaggaataa gtaaggcgag
     1801 ttataaacaa ctggttatag gtatatgtgt agctagtatg cttatatcaa atttaggtgt
     1861 tgattctata atagctatat cagttccaat cctacaaact atatatcctg ttttattagc
     1921 aatagttgtt atggagttgg ctggtaaaca tataaagaat gataatgctt ttaaaggtgc
     1981 agcatatgtt acattagtga ttagtctatt atcagctata aatggaatga caggagctgt
     2041 tccatttata caaagcttgc cactcgcagg tttgggcttt aactgggtaa taccagctat
     2101 attgggtgga atagttggta actttataaa aagcaacaaa caagtagcat aataatttgt
     2161 aattaattta attttataat atattaattt ttagtactat gggtaggttt aatctaaacc
     2221 taatctaccc atagtacttt ttagattgga ttggaatagc atgtatataa ttttttataa
     2281 tataattttt ctttgaagta tgataaaatt agtaatagtg ataagaaagt acaaatttag
     2341 gaggttttaa ttaatgctta gaaaaataac agcatttatg ctatccatat gtttaattat
     2401 tggacttaca ggttgtagtt tacttgtagg caagaaagat aaagagaaaa ttatagatga
     2461 ttttaaagtt gcagtggtaa ctcaaccatt atctgaaaat aaagtacaat acaatatggt
     2521 ggaagagatg acaaaagaat atgaggaaga gaataaaatt gataaagaca aagatggtca
     2581 aactaaagtt aaacaaacta taaaacatgt tgtattgcca gagaatttta cttctaatat
     2641 agatagtgct attaataaga tagtaaagtt ggctgacgat aaagaagttc aggctattgt
     2701 agtttcaact gaccaagcag ggcttttacc tgctttacaa aaggtaaagg aaaagcgtcc
     2761 agaaataata actatttctg cccctatggg agatgataaa aaccaattaa gccaatttgt
     2821 tgatgtgaat ctaggggtta gtgcagagga aagaggaaaa gtattagctg aacgttcaaa
     2881 agaaatggga gcaaaagctt ttattcatta tgcctctaca gatgatttga aagatgttaa
     2941 tatagccaaa agattagaaa tgattaaaga gacttgtaaa aatataggat tgccatttgt
     3001 acaagttaat actccaaata taaatactga agaagacaaa aacaaagtta agcagttttt
     3061 aaatgaagat attgaaaaac aagttaaaaa atatggaaaa gatataaatg tatttggtgt
     3121 aaatgagtat atggatgaag ttatattgac taaggcttta gaattaaagt atatagttgc
     3181 agagcaaagc aatccatcac caatacaaac gtatcctagt gttatgggtc taaaaatatc
     3241 tgaaaaagat gctcaaaatt atgataaaat aaacgacatg ataagtgaaa aggctaaggc
     3301 ttttggtatg tcaaatagac ttggtggata tcctatgcca atggatgctt ttctaccatc
     3361 agtagcaatt tatcttgcta ctgaaatggt gaaacaagat ttaacacaag aagatgtatg
     3421 tgacccagat tatttagagg cgtttacaga attaagattt ggaatcggtt cagaatttac
     3481 tcctttaaca gaggtacttt ataattatca atcagtaatt ttaagccaat taatttatta
     3541 atattaagga agtgtctcaa aatgaacttt ttagtttatg aggcactatt tttctgttaa
     3601 atcaaatata tttttatctt tttaacaatt aaaaagaagc ttatccctat gttgagatag
     3661 cttctttttt aatataaata agttttatta ttttataaaa aatttataga aaaataaaaa
     3721 attataatta tatataaatt ttgataaagt aaagatgaat tactataggt ttaaattata
     3781 ttattttcta ttatttttat gcctttctca gttattgatg agccttttct tcctttacta
     3841 gaaattaagt atccattgcg tgtcaaatat tctagcatat ttcttacttc gtattctgtg
     3901 acattatatt ccataaattt cattaacttt gtcctcccca ttggtttcat gtgcaatgat
     3961 tttaggatag aaatgcatat gtttttgtca tattggttta aatcattgaa tatagacata
     4021 ttacttgaca tatgattatt atttaatttt gaagatatgt tttgaggtaa atcatttatt
     4081 gtaagataat tggaacccat gtaagccata tattcaacac aatttctcag ttctctaata
     4141 ttccccatcc aagtgtatga atttaaagct tctaaaagac tcttatcgag ttttatgttt
     4201 ttatagggaa gttcttttaa aaatgattct aaaagaagag gtatatcttc taatctcttt
     4261 ctaagaggag gtaaatctaa agtgaacata cttatcctat aaaataaatc catcctaaat
     4321 ttattttcta taattaattt ttctaaatct ttatttgtgg cagctaatat tctcacatca
     4381 attggaataa tacttgtacc accaattttt ctaatttctt tttcttgtaa gactcttaaa
     4441 agtttaacct gtaaatcatg tggcatatct cctatctcat ctaaaaatat agtgccagta
     4501 tgagctaatt cgaataatcc tttttttcca cctttttttg ctccagtaaa agaaccatct
     4561 tcatatccaa atagttcact ttcaagtaat tcacttggta atgaagcaca atttattgct
     4621 acaaaaggtt tattttttct atttgaatga ttgtgtattg actgagtaaa ggcttcttta
     4681 cctgttcctg tttcacctgt tattaatata ggattatctg taagagccat tttttttgcc
     4741 cttttaatac aatcttttat aatagaacta tttccaataa tattattaaa tttatattta
     4801 gctacataac ctcttttgac agaatcactt cttatttttt gttctaatat ttctatattg
     4861 tttacatctt ttaatgtaat taagcaaccc tctatatttt tataaagata aaatggtttt
     4921 ttagaaagta tgaatttttt atctatttcg tctatgtata taatcatatt atcgatattt
     4981 aagttctttg taattttatc taagaaactc tttggcatat attccattaa ggaaggtgat
     5041 tttataagtt tttcatctag ttccaacata ttaagtaaag acttatttac aaaagtaacc
     5101 ttgttaaagg tgttgaaaaa tattagacca tcacctattt catcaatggt catgtaaagt
     5161 atagttttta atttagatat attatctaat gacgtattta agaaatcatg acttagtgtc
     5221 tccttggaca atttgtatag tttttctata aatttctcat tttttagttt taatactact
     5281 aataatgaca tatatgtatc taatgagatt tttctccaac ctaaatcaac aatatttttt
     5341 atgtattttg gtataaatct aatctgacct ggtgttatag ctgtatctaa atcatatatt
     5401 tcatcacaat ctggatatgt cggtacaaaa tctatatgat ttataccaat tttatataga
     5461 taagtagcca tctcaaatgc tgaagtttta ttggttgtca taagtaacga ttttgtacct
     5521 ggaggtagtt tcatcatttt ttcatatcca agttttgaaa gtgctttcgt tccatgaata
     5581 atttcaatat cttccttaaa atgttttatt aaaaaattac tagttaaagg tgatagagaa
     5641 agcactacct ctccctctaa attttctttt attccatcaa gtatattttg tgtttctatg
     5701 attatttcgt ctccaaaaaa gtttgttaat tcttctttgt agtggtcact atttaattta
     5761 tcatatgtta taaggattaa cttcttttta tccattttat ttctcctaac aaaatagtat
     5821 ttatttttaa agtatttaaa gtataaggta agttatatta tacaacaaat agtagtataa
     5881 atttatatat ttgattaaat aataggtttt tttaggattt tatcctataa aaacctattt
     5941 taaaattatt tttgcaagat aaggaaaata gctttttata ttgtaatgta tattt
//
LOCUS       DAEPLF010000200         5984 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEPLF010000200 DAEPLF010000000
VERSION     DAEPLF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.201"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            254..832
                     /locus_tag="KR314_003040"
     CDS             254..832
                     /locus_tag="KR314_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0329404.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            871..1449
                     /locus_tag="KR314_003041"
     CDS             871..1449
                     /locus_tag="KR314_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0329405.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLRHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1535..2164
                     /locus_tag="KR314_003042"
     CDS             1535..2164
                     /locus_tag="KR314_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0329406.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2154..4913
                     /locus_tag="KR314_003043"
     CDS             2154..4913
                     /locus_tag="KR314_003043"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBH0329407.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            5000..>5984
                     /locus_tag="KR314_003044"
     CDS             5000..>5984
                     /locus_tag="KR314_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329408.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSES"
BASE COUNT         2354 a          621 c         1177 g         1832 t
ORIGIN      
        1 tttcaaaata aaatatgtac ttgtttatat aaatgatata taattacata ttaaacatag
       61 cttttataca tttaataaag tatgtaaaat ctatgtaaaa agtaagaaat ataaacattt
      121 ttttaattat attttaaaaa tatcaagcaa gttttcttca caaatgaata aaaaatgtta
      181 taatatgatt aaagaaaaag ataaataatt agcaagattt gtttagctaa aattaacaag
      241 ggagcgataa attatgggta taacattatc aaaaggccaa aaggttgatt taactaaagg
      301 aaatccaggt cttaaaaata ttttagtagg tttaggatgg gatactaaca agtatgatgg
      361 tggatttgac tttgatttag atacagcagc atttttaaca ggagcaagtg gaagtgttac
      421 taatgatgga gattttgtat tttacaacaa tttaaaacat acatctggag cagttgagca
      481 tctaggagat aacagaactg gcgaaggtga tggagatgat gaacaaatag ttgttgattt
      541 atctaagatt cctggagaaa taagcaagat ttcttttact gttacaattc atgatgcgac
      601 agaaagaaga caaaattttg gacaagtaag taattcttat ataagaatag tagataaaga
      661 cacaaatgaa gagttaataa agtatgaatt aggagaagat tttagtatag aaacagctat
      721 agtagtagct gaaatatata agcacaatgg agaatggaag tttaatgcac taggttctgg
      781 ttttgaaggt ggtcttgctg cattatgtgg aaactttgga ataaacttat aatttcaact
      841 ataacaataa actaaagggg gatattttcc atggctatag tattaaaaaa aggacaaaaa
      901 atcgatttaa ctaagggaaa tccaggtctt aaaaatataa aacttggact aggatgggat
      961 acaaattcat ttgatagtgg ctatgattat gatttagatg taagtgtttt catggttgga
     1021 gaatctcaaa gagttgaaaa agatgaagat tttatattct acaataactt aagacatcca
     1081 tctggagcag ttgaacactt aggtgataat agaactggtg aaggtgatgg agatgatgag
     1141 gaaatattag tgaatttaga gacaatgcca aagcatatac aaaagatagc tgtaacagtt
     1201 acaatatatg aggctgctga aagaagacaa aattttggac aagtaagcaa ctcatatata
     1261 agagtattaa attctgaaaa tgatgaagaa atattaagat atgacttagg tgaagaattt
     1321 agtatagaaa ctgcaattac agtatgtgag atatataagt ataatggaga atggaagttt
     1381 agtgcagttg gagcaggatt tgaaggtggc ttagaagcac tttgtagaaa ctatggctta
     1441 aatgtttaat ttaagcaata taatatatta aatataaata acctatacaa ataatgtata
     1501 ggttatatta tataaaattt tgtggaggtt tagtatgtcg attaatttat caaaaggtga
     1561 taaaatagat ttaaaaaaat ctaacccagg actgagtaac atacttgtag gtctaggatg
     1621 ggaccctgtg caacaatctg gaggaggttt ttttaaatcg ttatttggtg gaggacaagc
     1681 tgatatagat tgtgatgcat ctgtatttat gcttaatcaa gaaggtaaat taagtggaat
     1741 aaaagattta atttattttg gaaatctaaa aagtgcctgt aaatcagttt tgcatacagg
     1801 agacaatcta actggtgagg gtacaggtga tgatgaacaa atcctagtta atttggataa
     1861 agttccatca aatattcata aattattgtt tgtagtaaat atatacaatt gtgtagatag
     1921 aaaacaacac tttggaatga tagaaaatgc ctatataaga gttgaagacc aaggtaataa
     1981 gaaagagata gcaaaataca atctatctga taattattct gaaaaaacta ctcttatagt
     2041 aggagctata tatagaaaag atggaagttg gcaatttaaa gcaattggtg aaggaacaaa
     2101 agatgcgggt cttaaagaag taatgcaaaa tttagacagg attgagtgtg catatggaat
     2161 ttaataatga tatcaaatat tataatgtag ataaagaaga agttacaaaa gacttatctg
     2221 ttaatcctga aaaaggttta tcagaatcag aagtcaagac tagaagagaa aaatatggtt
     2281 tgaatgaatt tactccaaaa gaagagggta gtttttggga tgatttaaaa gaaagtttaa
     2341 gtgaaccaat gatagttatc ttgattattg cagcagtagt aagtgcagta attggggaaa
     2401 ctcatgatgc aatagggatt gtaggtgcta tagcaatagg tatagcaata ggtatgatta
     2461 cagaaggtcg ttctaagaaa gcagcagagg ctctttctaa gttgactgaa aacatagaag
     2521 taaaagtatt gagagatggg gaagtacatc aaatttctaa gagtgaattg gtaccaggtg
     2581 atatagtata tattgaaact ggtgacatgg tacctgctga tggtagactt atagaaagta
     2641 taaaccttaa aattagagaa gatatgttaa caggagaatc tgatgacgta tctaaaaagt
     2701 gtgatgtagt agtttctatg gaagagatag aatctaaagg agcagtagta gtacaggagc
     2761 caatacctgc aaaacagata aatatggtgt ttggtggtac acttgtagct tatggtagag
     2821 gagctctagt tgtaacttca attggagata atagtgaaat gggtaaaatt gctcaaaact
     2881 tatcagaaac agatgagcaa actccacttc agataaaact tggaaattta ggtggtttga
     2941 tagccaaagt ttctagtgca gtagcaggat tactatttat atttatggtt ttccaaatga
     3001 taagtaagaa tgtattaaat gtagatatgt ctggaatatt accgttttta gaatcaatag
     3061 acccagttaa aacagcattt acagtatgtg ttgcgttgat agttgcagca gtgccagaag
     3121 gtcttccaac tatgataaat atgacacttg caataacaat gcaaaaaatg gctaaaataa
     3181 atgcattggt taccaaaaaa gaagcttgtg aaacgatagg ttctgtttct gtaatatgtt
     3241 cagataaaac tggtacatta actgaaaata gaatgactgt agaagttgca tatgttgatg
     3301 gcagatatat tgaaagttca gaagaggaaa taaatagtta ttttgaagaa aactgtatga
     3361 taaactctac agctgatgta gagcacaatg atggtgatat taagtattta ggaagtgcta
     3421 ctgaatgtgc attgctttta tattacaaaa acgtagatta tagacaagtt agaaaaaatt
     3481 ctaatatagt agctcaaaat ccttttactt cagattcaaa gagaatgact tctgttatag
     3541 gtcaagataa tcatcatgta ctattatcaa aaggagcacc agaagtctta cttgaattat
     3601 gttcacatat tcaaagagga aaagacatag ttccattaac tgaaaatata aaacatgaga
     3661 ttttagaaga aataaagaaa ttgcaaataa aatcaatgag aactttaggg tttgcatata
     3721 aagaaatctc acaagctgaa gaagaagcag cagtaactgc tgaaagtgat gctatgaatg
     3781 taagtgcaat ggaaaataat ttagtattta gtggttttgt tggtataaga gacccactta
     3841 gaaaagatgt tatagagtct gtaaatacag caaataaagc tggtgtttct gtaaaaatgc
     3901 ttacaggaga taatataaat acggcaagag ctataggtga agagttaggc ctattaaaaa
     3961 ataatatgag agtggttgaa gcttcatata tagatacttt agatgatgaa gaattaaaac
     4021 aagagataca aagtatttca atcgtggcaa gaagtaaacc agatagtaaa atgagaatag
     4081 tttcagctct gcaaaagagc ggggatgttg ttgcagtaac aggtgatggt ataaatgatg
     4141 ctccagcact ttcaaaagca gatgttggta tagcaatggg tatatctggt acagaggtat
     4201 ctaaaaacgc agcagacata attttaactg atgatagttt tagtaccatt gtaaaaggta
     4261 ttaaatgggg tagaggaatt tatgataact tccaaagatt cgttcaattc caattaactg
     4321 taaatgtaat tgcattttta gtggctataa tatctcaagt tatgggacaa gagatgccat
     4381 ttactacaat acaattatta tgggtaaaca taattatgga tggaccacca gcactagcat
     4441 taggtttaga gccagttaga gaccatgtgt taaatagaaa acctgttgat agaaaagcca
     4501 atataatttc taagtcaatg gtgtatacaa tagtattaaa tgcattttat ataacagcaa
     4561 tactaatgtt acaatctaca tttaatttct taggagttga tgaagctcat aagggaacag
     4621 taatgtttgc gttatttgct tttagcgcac tgtttaatgc atttaactgt agagagttta
     4681 atgcagatag tatcttccct aattttacac ataacaaatt agcactacaa ataatagtgt
     4741 tgacaggaat tgcacaagta atatttactc agctattcca agatttcttt aactctgtag
     4801 caatggattt tatgacatgg gtaaaagttt tatgtgtagc agcaagtgta attgtggtta
     4861 atgaagttgt aaaatgtata ataagactgg taaaaccaaa aaagactgct taaaacaaat
     4921 tttttagaag aaaacagact gactcaaaat atgaattttg ggtcagttct gaataaacat
     4981 ttaaattggg aggatagtta tgggtgactc aaaggtattt gaaaaaatat tctccagcca
     5041 atcagataga ggaaattaca ctccttcaaa gggatatcta agttatttta tttcgtatat
     5101 tggtttagaa gatgaagttt tgtacaatct cgagatattt aagacaaaac aaaatattga
     5161 tagcaaaaaa gatattgctt tatttactga tgttatagca aatcctagtg attttgatat
     5221 tataaattat tttaaaagtg gacttcaaaa atatagaact agcatggaag atgtagatat
     5281 aaatattttg ggatttgaag agattgatta taaaataaag caagcaatgg atagagtgtt
     5341 aaaagaagag gaaaaagaat ttactaatga tagagttaaa caaaatttta tagtgaaaat
     5401 aatggcatgg ataaaaattt atatcggtgc tttagatata aataaaaatg aagctccaaa
     5461 agtaatattt tatggagata taaaaaaaca tgaagtttat ttactcttaa tattatactt
     5521 ggcaggtttt gatgttttat atcttaatcc aaattcaaag agtaatatag atattttaaa
     5581 atcagaaaga tacaatatag agtttgaaga agccaacata atagaagaaa aaatttcttt
     5641 tgaagaaagg gttattttag gggaaaaaat agataaaagt tctgtaaaaa aagcttttac
     5701 tgttggggca gaagcatcaa agcgtataag tgaggaattg ttaaatgatg caggatttat
     5761 aaaaccttgg caactccaag atagaaaaat aaaaaatcta cttctaagtt caactgttga
     5821 tgaaatatca atatactgga atcaaccact taaattaaga ccaggattta agtttaatga
     5881 tgctattgta gaagctccaa attttctttc taagattaat ggaatatata atgataagaa
     5941 tgaatatatt aagtttttag atttattaag agattcggaa agtt
//
LOCUS       DAEPLF010000201         5970 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEPLF010000201 DAEPLF010000000
VERSION     DAEPLF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.202"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(61..780)
                     /locus_tag="KR314_003045"
     CDS             complement(61..780)
                     /locus_tag="KR314_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBH0329409.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYSGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGTRVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(898..2172)
                     /locus_tag="KR314_003046"
     CDS             complement(898..2172)
                     /locus_tag="KR314_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBH0329410.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(2372..3739)
                     /locus_tag="KR314_003047"
     CDS             complement(2372..3739)
                     /locus_tag="KR314_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0329411.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIRYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(3989..4453)
                     /locus_tag="KR314_003048"
     CDS             complement(3989..4453)
                     /locus_tag="KR314_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBH0329412.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(4458..5087)
                     /locus_tag="KR314_003049"
     CDS             complement(4458..5087)
                     /locus_tag="KR314_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBH0329413.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADKVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(5121..>5970)
                     /locus_tag="KR314_003050"
     CDS             complement(5121..>5970)
                     /locus_tag="KR314_003050"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBH0329414.1"
                     /translation="ISDYIKLMRIKQYIKNFFVFSAIIFSNNILNINLFLNTFIAFVC
                     FCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILFVVLALISVCLSTV
                     VSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAGCVAISVSLSNWII
                     LCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMMIVILSSTIVCYSL
                     YSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDNALKINVILWIITC
                     LIILIF"
BASE COUNT         2021 a         1049 c          620 g         2280 t
ORIGIN      
        1 tgtcataata attcatatag ataaacttat ttttactcaa attttttcac tcccaagatt
       61 ctaaaaattt atatcaaaaa attctataat ttcatcttca tgataattta tgactaattc
      121 atctggaaaa tctactgctt ctaaaacttt ttctgcattt tcaaattctc ctatagaata
      181 acatatatga gagtcagtac caagaattac tcttgtacca tatgttttac acaatgtcaa
      241 catatgactt acattttccc aagtacccgt tctatgagag tttgaactta atgatgaatt
      301 atttatttct aaaaggatat ttttatcttt agcttttttt actataggtt cataatctaa
      361 aggataccta ctgtcatctg gatgtccaat tatttttacc ttaggtttat ccataacttt
      421 taaaatagca tttgtatttt cttcttttgt accagaagct atacaaggtc tatgcaaact
      481 tgcgattata taatccaaat gttgcaacat atcttcttga acatctaaat taccactata
      541 atccattata tttccttcta ttcccctaag aatctttaca ccttgcactt cacgtggaag
      601 caaatgcaaa ttataaaaat agaatggatg tggtcctcct ggcatattag gtccgtggtc
      661 tgatattcct aagtatttta tattcttctt actcgcctct tcgacatttt ccttgaaagt
      721 actatgtgca tgtccactta ttagagtgtg acaatgtaca tccataatag actttttcat
      781 taaatttcct cctaatattt tacataaatt cgtgaaaata gctaaagggc tatttcaaaa
      841 ctaaagctgt cataatagac agtgtaacgt tttaaaacag ccccattttc aattatttta
      901 cacttctaaa tctttaaatt tatctttaaa taattctcca atacttccta aagattcctc
      961 tacttctagg tattctgtta tatcctcttc tggctctcta gttgcttctt ttattgaaac
     1021 caacattctt ttagtctttg gtttaaaatc aagaatctta actttaacct catctccaat
     1081 attaacaaca ttagtaactt tagccactct agtatcactc aattcagata ttggtaaata
     1141 cgcatctaca ccttctttaa tttgaacaaa agcccctttt tctattattc taagaacttt
     1201 agcattgaca acatcatcaa tttgtacttc ttttgctatt aaatcccaag ggtcattatt
     1261 tatgtctttt aatgccaagg caattttttt agtctcttta tttacatcca acacataaac
     1321 ttctacttct tgtccctctg aaacaacatc ttcaacactt tcaactcttg tccaagctaa
     1381 attatttata tgtgctaatc cttcaatccc accaatatcc acaaatgcac cataaggcat
     1441 tatctttgtc accttaccat ctcttttttg tccaacttca agagaattaa ataagtcttc
     1501 tttagctgct ttgatacgag ctttttcttc ttctctttct tgcttaattc ttgctctttc
     1561 agcttctttt tctgccttaa tttgagctct tctttcttct ctctctttat ttatacgctc
     1621 ttgtaataca tctctatgag atgctacaag tctatttttt ctaacatcca attcttttat
     1681 ataaacttct aagttaagac caacaaattt agatgtatct gtaataaatt ttgtatcaag
     1741 ttgagaaatt ggtataaaaa atgtatatgt attgtaatta gcaaatacac ctttatctat
     1801 attcttttca acatttacag ttattattct atgttcatca taagcctttt gtaactcggc
     1861 aaaatcattt cttttgtcaa gttgtaattt agataaagtt attgtaccat ctttttgaga
     1921 tacttttgtt attacagcag ttatttcatc acctatgtgg tatatatctt ctatatattg
     1981 atttttttct atgttcaatt cacttatagg tattattcca tcaaacccat agtctaatcc
     2041 taattgtact tcatcatttc tcactgcagt tatttttcca gttgtcaatt ctccaacttt
     2101 cactttgctg aatacttgtt cttgctggtc aagtaattct tgcattgtta aatcattttc
     2161 cattataaac atcctttcct cttattgatt atcatatctg taaatattat accattaata
     2221 ttgcaaatac ttacattttt cttactataa tttttaaaat aaattcaact tcatatattt
     2281 atacattata gaaaatatct tattaaatta aaatacaaat tctaattttt atctaataaa
     2341 atattttcat aaaaaattta tattgtatat cttattcttt tattgcttta ggttttgata
     2401 gaaaacattt ataaaatata aagaaagaaa ttgctgttat agtagcagct gttatatctg
     2461 caactggctc tgctaaaatt actgcaaaaa gtttttcatt aaagaatgtt gggagtataa
     2521 atattaaagg tattaaaagt acaatctttc taagtagagc taaaaccaaa gatattgttg
     2581 cttttccaag tgctaaaaat gtctgttgac aagctatttg aatcccaaac ataaaagcac
     2641 cagcaaaata tatttttata ctccaactag tcatactaag taactgcgga ttcttattaa
     2701 atatactaac aaaaaatgca ggaaatatca ttaataaaag ccataaaata gtagaatatg
     2761 taaaacatga caataaacac aacttaaatg tcttttttac tctatcaagt tgacctgaac
     2821 cataattata acttattatt ggttgtgctc cttgtgtaac ccccatatta ggaagcataa
     2881 gaatttgcat tatactgctc attatggcca ttgaacctac tgctaaatct cctccataca
     2941 tttgtaattt actatttaag gctataagta ctaaactttc tgtagattgc attataaatg
     3001 gagagattcc caagccaagc actggtaaga tgatacttgc ttttggtatc atatacttct
     3061 tttttatttt aagtatactt ttctttccaa agagaaacat aagtacccaa atagcagaaa
     3121 cgccttgtgc aattatagta gctaatgcag ctccttttac acccatatta aagccaaata
     3181 taaaaatcgg gtcaagtatt atgtttatta aagcacctat agcaactgtc atcattccag
     3241 ttgtagcaaa cccttgggta tttataaaag tattcatacc cattgaaatc tgaacaaaca
     3301 ctgtacctat taaataaata tttaaatagt ccattgaata tctaattgta gattcacttg
     3361 ctccaaacat ccaaagtaaa ggctcttttc caatcttaaa acctatagtc aaaagtattg
     3421 ctaaaatcac cagtacagaa aaactgttac tcattatttt ctctgcctca tcattgtttt
     3481 gctcacccat ttttatagca gctaatggac ctcctcccat accgattaaa taactacatg
     3541 cagttattat aattattatt ggaaaggcca ctccaacacc agccatagca acttctccat
     3601 tttccattct accaataaaa atcctatcga ctatgttgta taaaacattt acaatttgtg
     3661 caataatagc aggtgtagca agtttaaata ataatttacc cacactttca ctgcctaagt
     3721 tagtgtttga attttccatt ttaaaacctc ctcaatccaa taattaaaat gtatcactaa
     3781 aattttattc tatataaaaa tttcatttta tcagcagaca aatttagagt actttcccta
     3841 caagtaatac tatactatat ttcttttgcc atagtaaaca ttttagaact aactgaattt
     3901 aaatacttta tctcattttt tatttattat taaaaatttt tcacatgtac tcataaaaaa
     3961 actatccaag tatattactt agatagtttt atattatatg taaagaaaga gctgaaacta
     4021 taacccctga taacaaattg ccaagaaata ctcccataaa agcatctttt attctcattt
     4081 ttaatatgga agctatagca gatgcagtcc atgttcctgt agttggcagt ggtataccta
     4141 caaatagtat tataccaatt atcgaaacac tttttaattt cttcattctt gagtttattt
     4201 ttctatcaat tacatcagca atccctttaa atagtttctt ccctcttaaa aattgtaata
     4261 catgtctgaa tgtcaatatc aagggaatcg ataccaatgt agaacctatt acactaaata
     4321 cataaaccca tataggattt aagcccattg caatacctat aggaatggct cccctcaatt
     4381 ctgttacagg aaccatagat aaaattaata tgtgtaaata tcccaaacta ttccctccta
     4441 ttctaagtga cattaagtta tgaatttatt tgctctataa cctcaccatc ctttttacta
     4501 actaaaaatg catttttagt caatgaaagc attggtaaat cactcttaga gtctgaatat
     4561 gcatatgaat tttcataatc aatctctatc ccaagtttac ctaatacagc ctttattctt
     4621 tttacttttt caattccctt acagttttct ccaatgaatt gatttttata tcttgaattt
     4681 tcataaaaaa actccgtgcc tataaccttg tctgcaagac cataatactt aaaatatttc
     4741 atatatgcat atggagatgc agttatcata attataacat tatcttcttc tttatttttg
     4801 taaatgacct ttttcataga ttcgttaatt tttttcatta tgcaatcatc aaaaaacttt
     4861 tttaaatctt cttcactaaa atctactatt ggataaaaga ttgcctcttt tgccttcttc
     4921 aagggtatca ttctaattaa atacataaat agaataaaaa taatgtatgg caactttata
     4981 atagttttaa aggttttatt ttttaaagag taaataaaaa aatctatcat gctatcagta
     5041 tttataacag tataatctac atcaagtaat aatacacttt ttttcataag tatttcccca
     5101 tcctatcact aaaaatatca ctaaaaaatc aatattatta aacatgtaat tatccataag
     5161 attacgttta tttttaaagc attatctttt aggacaatat cagtaggatt attttcattt
     5221 gtagtgttaa ttatataatt atatctaagt acaccataaa ctacaaatat tgttgtaaat
     5281 atcatatgtg gcttttctgg attacttgta gaatacaatg aataacatac aattgtagaa
     5341 cttaatataa ctatcatcat ttgatttaga ttttctatgt cataatcttc caatattttt
     5401 ctatgttcaa ctgcatcatc tcttagagtt tctatttctt ttttacgttt tccaagagct
     5461 aagtacagag ataagaaaaa tgtacataaa attatccaat ttgataaact aacacttatg
     5521 gctacacatc ctgcataaac cctaagtata aaaccaagag atattgaaaa cacatctaat
     5581 agtattatat tctttagttt aagagaatat aatatattgt ttatcaaata gagactaaga
     5641 attatagaaa gattaaaact aacaactgtt gataaacaga ctgaaattaa agctaatact
     5701 acaaatagta tattagcact ttttttgctt attcttccac ttgccactgg tctattacat
     5761 tttttaggat gttttttatc tttatccatg tctatagcat cattcagtgc atatactgat
     5821 gatgacatta ggcaaaaaca cacaaacgca ataaacgtat ttaaaaataa atttatattt
     5881 agtatgttgt ttgaaaaaat tatagctgaa aatacaaaga aattctttat atattgcttt
     5941 attctcatta actttatata atctgaaatt
//
LOCUS       DAEPLF010000202         5918 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEPLF010000202 DAEPLF010000000
VERSION     DAEPLF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.203"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            56..577
                     /locus_tag="KR314_003051"
     CDS             56..577
                     /locus_tag="KR314_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0329415.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            956..2131
                     /locus_tag="KR314_003052"
     CDS             956..2131
                     /locus_tag="KR314_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0329416.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQAYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            2166..3704
                     /locus_tag="KR314_003053"
     CDS             2166..3704
                     /locus_tag="KR314_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBH0329417.1"
                     /translation="MQGALKENKKKSGGGFLGWIERVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQVI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            3736..4938
                     /locus_tag="KR314_003054"
     CDS             3736..4938
                     /locus_tag="KR314_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0329418.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFIQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     SEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     IWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(5117..5716)
                     /locus_tag="KR314_003055"
     CDS             complement(5117..5716)
                     /locus_tag="KR314_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0329419.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
BASE COUNT         2235 a          718 c         1064 g         1901 t
ORIGIN      
        1 gaatcttatc aattagaata tgcatagaaa caagtggctt aaaaggagac gttatatgga
       61 agagatagta aagaaggaac aagcaagtat gaatgatatt tttttgtatc ttaaagctaa
      121 atcaaactca ttatataaat ttgtaatatt atataatgag tttatcaata aacctaaaga
      181 ttatggaact ggggaactta taaacatggt tgaagtacat atgctaacag cgattgaaga
      241 aaatccagga ataagtccaa ctcagttagc taaaatatgg aacagaacta aaggtgcaat
      301 atctcaaact atatctaaat tggagaaaaa gggttatata actaaagaaa agaaagaagg
      361 aaatgcaaaa actatattac tatatccaac cacatcagga gtagagttaa gtaaggcaca
      421 taaactatat gacaccatag atgttacaag aacatttgaa tacttaatca aggagtgtac
      481 attagaggag atagatagtt tttataaagt aattgatttg tttattaatt tattagaaga
      541 atctccgcag gaaaataatg attctgaaaa agtgtagcta agattgaagt tgaataaaga
      601 gaaatagata ttatagaagt agtacttaag tacacattat ataaaaatat aataggtata
      661 ggattaaaaa gtttagtggt ttaaagttta accactaaac ttttttaatg tctataatct
      721 actattattt aatttactaa aaataaaaaa actcattata ttaagatgaa aatagaaaaa
      781 taactagaaa aatactataa tatatcagct taatacagaa aaaagttaaa aatattgaga
      841 aaaataaaaa tgaaaacgtt ttatattgac atagagaaaa agtagtgata ccatttaaat
      901 atagtttagt agctaaacta tatttaaatt ataataacta ttttaggggg atgttatgaa
      961 tttaaaacaa ttaagtgaaa aatacgaaga atatgtaatt caaatgagaa gagaatttca
     1021 taagtatcca gaattatctt ttaaagaaat aagaacaacc caaagaataa gggaagaatt
     1081 acaaaaaata ggggtccctt ttgtatctat tgaaccaaat atcgtaatag ctactataca
     1141 aggcaaaaat gaaggtaaga caattgcaat tagaggagat atagatgcgc ttccaatgca
     1201 agaagattca gatgttgaat acaaatcaga atatgatgga ataatgcatt cttgtggaca
     1261 tgatgctcat ggagctatgt tactaggact tgcacatatg ctaaatgata taagaaaaga
     1321 tttaaaaggt agagtattat tagtttttca agcagctgaa gaagttggtg gcggacacaa
     1381 ggagataata cagtacttta aggaaaatgg aggaccagat agattaatag ctactcatgt
     1441 ttggtccgat attgaagctg gtaaaatttc tgttgaacca ggacctcgta tggctggtgg
     1501 gtctggttgg agaatagaca taacaggtag aggaggtcat ggttctagac cagatcaaag
     1561 catagatcct ataaagccag cttgtgatat agttttaaaa gtatcttcta taccagttaa
     1621 tcatataagt gtactagaac aatgtgttgt acatgcatgt gcaatacatg gaggaactac
     1681 aataggaaat gttattccaa acaatgcaga ggtaataggt ggatatagat attttactga
     1741 agaatcaggc aaaaaagtgt ttgacattat aaagcaaatg agtaatggtg ttggacaagc
     1801 ttatggagtg gatgtgaagg ttactcatac tggaggcata ggacctgtta tcaatgatga
     1861 agaagcagtt aagatggcta aagaaatagt ttctaatata gatggattgg agttagattc
     1921 ttatgagcca atatgtgcat cagattgtta tggtgaaata ttaaaagagt attctggttt
     1981 ttattgttat ctaggggtag gtaacaaaga aaaaggaata atatatgcac aacaccatcc
     2041 taagtacaat atagatgaag atacattaaa gttaggttgc gaatttatgg ctaaatatgc
     2101 agttgaattt ttaaataaaa ataataacta aaataaatcg aattttataa atggggggga
     2161 tgaaaatgca aggtgcatta aaggaaaata agaaaaaaag tggtggagga tttttaggat
     2221 ggattgaaag agttgggaat aaaatgccac atcctctagc attatttaca tatattacat
     2281 tgatagtagt agcattatct gcaatagcaa gctttttagg tttttcagca acaagtcctg
     2341 ctacaggaaa gttaataaaa gttatgaact taataagtgc aaaaggttta gtactattta
     2401 tgcaagagtt tgttaagaac ttccaaaact tccctgtgct tggggtagtt ttagttatgg
     2461 cagttgccac aggagtttgt gaaaagacag gatttttttc aactgccata aaaatgagtt
     2521 tatcaaaagt taaggggaat gcggttgtat ttataatagc ttttatagga gtatttgcta
     2581 atcaagctgg tgatgctgct tttgtattag taccaactct tgcaggagca atattttatg
     2641 gattaaatag aaatccatta gctggtatat tctgtggatt tgcatcagtt ggtggtggat
     2701 ttgcaacagc tgtaattcct gggggatggg acgttacgct aacgccaata actgtatcag
     2761 ctgctcaaac tattcaacct gcatttgata tgacattatt agctagttat tatgcactat
     2821 ttgtatctgc gtttatagta gctacagtat caacaatagt aacggttaaa tttatagaac
     2881 ctaaattagg taagtatact ggtaaaccag aaggtataga tgataatcaa ggttttgagg
     2941 tcacaaaaga acaagcaaaa gcagcaaagt tagcaggttt tacagtttta gcttatgtag
     3001 cgttattaat tgctttgtgt ataccagcta atagtttttt aagaagtcca gaaggttctt
     3061 taatatttgg agcaccactt atgtcttgct tacagttttt tatagtaata ctattcttct
     3121 tgccagggat tgtgtatgga atgagggttg gtaaaatcaa gactgtaaaa gacttaaatg
     3181 atatattatg tcaatcaatg gcagcaatgg caccgtttat cgtacttgca atagttatag
     3241 gtcaattctt aacgctattc tcagtgtcta atttagcaca ggtactagct ataaaaggtg
     3301 gagcattact caactcattg ccactaccac cacaagttat aatactgcta ttcttagtac
     3361 tagtagcgtt tataaatata tttgttataa gcggaagtac aaaatggatg atatttggac
     3421 cagtatttgt tccgatgtta atgcaattaa acatacatcc agcatttact cagtttgtat
     3481 atagattagg agattctgta actaatcatt tatcaccatt aaatgcattc tttataatac
     3541 ttttagcaac agtccaaaaa tacgataaaa atgcaggtat gggaactata ttctcagcta
     3601 tgatacctta cactataggt tactttgtag tacttgcagt tttagtagta gtttggatga
     3661 caataggtat accagtagga ataggtggac aagtttggtt ataaaacata tttaataatt
     3721 ttgggaggaa agaaaatgga aacaactgaa ataagataca attttgatga agtaatagat
     3781 agaagtggaa caaactgtct taaatatgat cttaagagtc aatttatgcc agaatctcca
     3841 gaagatgcac taccactatg ggtagcagac atggattttg catgtgctcc tgagataatt
     3901 aatgcaatgc atgaaagaat agacagaaaa atatttggat atagctctca acaaactgat
     3961 gattattatg aagctgtatg tggatggttt aagagaagat ttgattggaa aatagaaaaa
     4021 gagaatatat tctttagccc aggggttgtg cctgcgttag gatttttcat acaaatatta
     4081 acacaaaagg gtgatggtat cataatagaa aagcctgtat actatccttt tgaagataaa
     4141 ataactaata atgaaagaaa agtagttaat agtcctttaa agtttgaaga tggtcattat
     4201 tctatggatt atgatggatt tgaagaatta gctaaagatg aaaataataa ggtgtttata
     4261 ttatgtagtc ctcataatcc agttggtaga gtatggaaaa aagaagaact tagaaaaata
     4321 gtagacatat gtaagaaata tgatttgtgg ataatatctg atgaaataca ttgtgatatt
     4381 attcgtaaaa gtgaaaagca tataccatta gaagtagcat gtcctgatta taaagataga
     4441 ataataactt gtacagctcc aagtaagagt tttaacttag ctggtatgca attatcaaat
     4501 atagtaatca ataataagga attacaagaa aaatggatgc ttgaaacaga ttctaaatta
     4561 gctatattcc tacctaatcc attcgcaata gttgctacta aagttgctta taatgattgt
     4621 gaagattgga tgaatcaagt aaatgagtat atagatggta atattaaata tgtagaagat
     4681 tttgttaatg agtatatgcc taaagtaaag ataataaaat ctcaaggaac ttacttaatt
     4741 tggttagact ttagagggta tggattagat gcaaaacagc ttgaatatgt aatgcttaac
     4801 aaggcgaagg tattgctaga tgaaggatac atattcggag aagaaggagc aggatttgaa
     4861 agaataaatg ttgcttcgcc tagaacaatt atagaagagt gtatgaatag aataaaatgt
     4921 gcatttgaag gattataata gtaatatcta aataatatat ttgtgtattt taaaaaaaga
     4981 ctatgttaaa aaaatatttc aaagcatagt cttttaaagt tttatgatga gtttaaaaaa
     5041 tttcaaaacg ttactattat tgataatctt cacttatatg tttatattag aaataattcc
     5101 acaatatgct cattttctaa agttgcactt ttatacacct tgcttcacca atatcatgtg
     5161 taaccaaaat agtagactta ttctcattct taataatact ataaacatca tcacaaacta
     5221 ataatctagt ctgataatca agagctgaaa aaggttcatc taaaagtaga atatctggat
     5281 ttacagaaag tgttctaata agcgcaactc tttgtctcat acctccagat aattcttttg
     5341 gatacatact tctaaaatcc cataatccat aagtctttaa aaggctttca actctatcta
     5401 aagatttttt atcttttttg ccttgaattt ccaatccaat agttatatta tccattatat
     5461 ttctccattc aagcaaatta tctttttgaa acatatatcc aatatttcca gtaatcttta
     5521 tatcaccact agtcggtttt aaaagattag taagaatatt taatattgta gatttaccac
     5581 ttccagaagg accaagcaaa gttaatattt ctccttcttt caagttaaaa ttgacatcct
     5641 ttaatacttc aagctcgcct tccttagtgt agaagttcat atttatattt ttaacttcta
     5701 ctatgttgtc attcattatg ccacccccta taacttttag ctcgtatata ttatgttatt
     5761 aacaatatga cctatttgta ccatttttca taaatataat ataaatattt tgtttattta
     5821 cgtattttct tagtaacttg tacgtataaa ttataataaa taaattataa ggtaggggat
     5881 aaatttgagt aacataataa cgaatttaat agagtcag
//
LOCUS       DAEPLF010000203         5876 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEPLF010000203 DAEPLF010000000
VERSION     DAEPLF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.204"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            147..1241
                     /locus_tag="KR314_003056"
     CDS             147..1241
                     /locus_tag="KR314_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH0329420.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
     gene            1392..1748
                     /locus_tag="KR314_003057"
     CDS             1392..1748
                     /locus_tag="KR314_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329421.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(1841..3109)
                     /gene="eam"
                     /locus_tag="KR314_003058"
     CDS             complement(1841..3109)
                     /gene="eam"
                     /locus_tag="KR314_003058"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBH0329422.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(3511..4893)
                     /locus_tag="KR314_003059"
     CDS             complement(3511..4893)
                     /locus_tag="KR314_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0329423.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            5018..5704
                     /locus_tag="KR314_003060"
     CDS             5018..5704
                     /locus_tag="KR314_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBH0329424.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEGVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
BASE COUNT         1960 a          830 c          770 g         2316 t
ORIGIN      
        1 agcataaagt tttaacgtat ttataatttc atattaatca tactatatta atactgcttt
       61 ttattgtata ataagctata aatattaact ttaacttaga tttaagtata tctaaattta
      121 ataaatataa gaaaggttgt gttattttgc aagataaaaa aatttatcta aatattgtac
      181 tcactgtagt tgtttcatat atcttgatta aagttattga taattacaaa tatttttttg
      241 gagtgataag tttattaatg tcactactaa caccatttat aatagcattt gtattagcat
      301 atatattcaa cccactagta aagttcttag aaagtaaatt aaattttaag agaatatatt
      361 cgttacttct tacttatggt gtactcatag ttatactaat ttcctttata ttatttacag
      421 ttccatccat agtaaatagt ttagctgatt tagttgctca aattcctaca tacatagata
      481 aaacagagca gttcttattc gacttaggta agagtttaca atcagttgac cctaatacat
      541 taaaagagta tggtgacaaa attatgagtg taatgcctaa atttagtaac ttgcttattg
      601 gttcacttgg tgggatattt agcactacat tttcagttgg aaaatttata gtacaatttc
      661 ttttaggttt tataatttgt ttctatatat tattagaaaa agaaaaattc ttactgttta
      721 caaaaaaagt agtttatatt tccctaggta aaaaatatgg agactttata atagaactat
      781 gtcaaagctt aaatttaaat attggtaaat actttacagg taagatttta gattctttta
      841 tagttggagt actttctgga attggtcttt actttttaaa atctgaatat gcactattat
      901 ttggtacact tatgggtgtc atgaatatgg ttccatattt tggtccagta ataggtatgg
      961 caccagttgt aataattaat ttattctcca atccaacaat tgctttaact tctttaattt
     1021 acttaataat aattcaacaa gttgaagtta catttataga gccaaaaatc gtaggtggac
     1081 aactaggtct tagcccattc tttacaatcc tagcagttac tgttggtgga ggattctttg
     1141 gtatacctgg tatgattcta tctgctcctg tgatgggcgt tataaagata tacttctgtg
     1201 agtttgtaaa tcgtagacat gataaaatac aaatggaata aaaatacaat ttaattacga
     1261 ttttgtaatc aaaagttaca gctgtgtaac tgtaatatta ttctctttaa tggtaatatt
     1321 taattatagg aaacatacaa tattattaag taatgaatct tatataatta aacatgaaag
     1381 agggttttag tatgaaaaaa gcacttatat ctttattagt tttatcttgt ttgttattaa
     1441 ctgcaccagt aaatgaatct tcttatgcag attctcaaaa gaatgttcct gtaagccaaa
     1501 atactaaatt gactcaagaa caagctaaag aacttttagt aaaatacaac aatgaagttg
     1561 attacatata tcaaggaaat gctagtgatt ttgaagcatt aaaagctaaa aatcttaatg
     1621 gttatgtatt tttaccagat gcagatggtg atattggtta ctttgtaaat gaaaatactt
     1681 ctgagatata ttacttccac ccaagtggat atcttgaatt agttaatcaa caacaaaata
     1741 ataaataact aataaaaaat ggatgcaaat tgatataaat ttgacatcca ttttcttttt
     1801 attttaaagt tgtttctttt tataaacttt aaatactttt ttaatcctgt gcttgctctt
     1861 ttataagttt ctttatatct acagcaggtt catcttccat ttttataact tttccttccc
     1921 atgttctaag cataacgtaa tctgttcctt ttgatacaag gtattgcgga agcaaaggag
     1981 tctttcctcc tccttttggc gcatttacta tatatgttgg tatagccatt cctgatgtat
     2041 atcctcttaa atactccatg atttcaagac catcatctac tgatgtattg aaatgttttg
     2101 ttccctttac atgtttactt tggaatatat aataaggttt tactcttatt tttagtaatt
     2161 cttgatttaa acatctcatt acaaatttat cattatttat tccatttaat aatactgcct
     2221 gatttcctaa tggaactcct gcatttgcta acttttcaca agcttcttta gattccttag
     2281 ttatttccat tggatgatta aaatgagtat ttatatatac agggtgatat ttttttagca
     2341 tattgcaaaa ttcatctgta attctttgtg gcattgtaac aagagtccta gtacctaatc
     2401 taacataatc aacatgtggt atctctttaa gttggctaag aatccactct aaataatcat
     2461 cttttaatgt aagagcatct ccaccagtta ctagtacatc tcttatttct tcattttctc
     2521 tgatatagtc tatagattct tggataatag cttttgactt atgagaatct tgttgtccaa
     2581 tatttcttct tctctgacag tgtctacaat acatagcaca ctcatttgtt acatttatta
     2641 ttaatctatc tgggtatctt ctagttatac atcctgctgg gtttgtatac tcttctccca
     2701 ttgggtccaa atcttcttgc tcatcttcca actctatatg tgttggtata gacagtaatt
     2761 ttattgggtc acatatatct tctgggtcta taagactcaa ataatatgga gatattgccc
     2821 atctaaattg agtaccaacc tcttttatat attctttttc ttttttagtt agagttataa
     2881 tttttgataa agtatctaca tctgttattc tatttgataa ttgccatttg tagttattcc
     2941 aatcctcttc agttccattt aagatactta atattttctg ttttcgttta tttatctctt
     3001 cttctttttc aagacctctg ttaatcgttt ttctagcctg aatataatca tcaatttttg
     3061 attttaattc agcagctctc tctaaggata ttctagtttg ttcattcata attttttgct
     3121 atcaaagtta actacttgta ttatttgata gcgtcctcct ttctgatgtg tttatatcat
     3181 tctgataggc agatttaatt ttgccatata gattttataa caaatgaaat tgaattaatt
     3241 tttgcatttt ttctgttatt tcgcacattt atgaatattt ttcaaccttc gtcaataaat
     3301 tacaataaat tatgcatttt ttttaacaaa tgattaattt tttctatttt taagaattat
     3361 tttaccataa aacgattata ttttcaagat tattaaaatt aaatttttta ataaaaagta
     3421 aagtatttca ttaaataata aaagcggagg tttattataa taaatttctc cgccttttta
     3481 tttatttaat taaataatag taaaataaat ttaactctta agaagtgact ttattatatt
     3541 aagccctttt tctaattctt ctacatcttt tggagctgtt acacaaatac gaactgcttt
     3601 tggaactgta gagtttccta ctgaaaatcg ttcagcacaa tatacttgaa ctcctagatt
     3661 ttttgcagaa atttcaaatg attttccatc caattcatca ggaagaagta accaacgaaa
     3721 actgcaattc ttatcaccaa tcaaattata atctgacaaa actttatcag caacttcatt
     3781 tctttctata attgcatctc ttttctcttc tataatttta tctataatag gagaatataa
     3841 taaacgatgt actatttctg ctgtaaatgg agaaaccatt agattcatat tatatagtgc
     3901 attttctaaa ttttctatat atcgttttgg agcaactaca aatgctactc taagaccagc
     3961 acataaaaat ttagatgtac taaaaatata tatagtatta tcactagcta aagaaaatat
     4021 gggagtttgt ataccgtctc taaaaatgct atttatagca tcttcaataa ttattatatt
     4081 atattgcttt gctatcttgg ctatcttttt acgagataaa tcagacatag tatgtgtcgt
     4141 tggattatga taatctggta taagatatat gccttttata ttttcatttt tacaataact
     4201 ttcaagatat tcaatcgata tttcattatt ttcttgagga attgggacta attgaatacc
     4261 tatcatttta gctattgact taattccaga aaaacttaaa gaatctgtag caattctatc
     4321 accagcttta aataatccta gtaaagttgc acatatagca ttttgccctc ctgtagataa
     4381 taatatattt tcttcactgg tgtatacatt atttctttct atccattttg caattgactt
     4441 tttttgcata tatgtgccag atggagacat gtattgtaaa aacctatcca tttctggttg
     4501 ttttagtaca tttttaataa agtcaataat atatgtattt tgctcatatg gaggatgaac
     4561 tgtccctaat tcaattatgt cttttgattc cgtttgatat aataatgtat tacttacatt
     4621 tacatcagaa gaaatgaatg ttccctttcc cacttttgca catattagtc ccttttcttc
     4681 gcaaagttta aatgctcgtg ttattgtact taagtttaaa tctagaaaat ctgctaattc
     4741 ccgttgtgga ggcagtttat ctccaggatt taaatttcct cttttaatat cttcttctaa
     4801 taacattgct aaagttttat atattggtgg ttttcggtta tctaattttg gtttccatgt
     4861 cataggatag ttttcaaaag aatttattgg catataatta ctcctcttaa ttataattgt
     4921 actccataca attttattat tgtatgcttc tattttagat gctatacttg tatttgtaaa
     4981 tagctatttc aaaataaata aataagagag gtataatatg aagactatag gcttaattgg
     5041 tggtatgagt tgggaaagta ccattacata ttatcaagtt attaatacag taataaaaga
     5101 aagattaggt ggactacact cttctaaatg tatactttac agtgtagatt ttcaagaaat
     5161 tgaagaatgc caatctagtg gaaattggga aaaaagcgcc aaaattttag ctgatgctgc
     5221 aattaaatta caagaggcag gggcagattt tattgttatc tgtactaata ctatgcataa
     5281 ggtatcagat aaaatacaag aaagtataca tattccatta cttcacatag cagatgtaac
     5341 tgctacagta ttaagggaaa aagaaataaa aaaagtggcc ttattaggta ctaaatatac
     5401 aatggaacaa gatttttata aaaatgtaat tattaataat ggaattgaag tcttaattcc
     5461 aaatgaagaa gaccgtataa tagtaaatga cacaatattt aacgaacttt gcttaggaat
     5521 tatatctgaa tcttcaaaaa aagctttctt atctattata gataagcttg gcaaacaagg
     5581 tgcagagggt gttatattag gatgtacaga aataggtctt ttaattaaac aaaatgacac
     5641 ttctattcca ctatttgata cgacagtaat acatgcaata gaagccgcac taagttcaat
     5701 ttaagacaat attgattaat aaaaaaaata tctatagatt tgtgtaaata agacttttta
     5761 taatttatat tactagagat tttaaaccta tatattgtaa caaaaaaagg atagtataat
     5821 taccctatac tatccttttt cttaatttaa tatttaaata aactcaacac tttcaa
//
LOCUS       DAEPLF010000204         5870 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEPLF010000204 DAEPLF010000000
VERSION     DAEPLF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.205"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..161)
                     /locus_tag="KR314_003061"
     CDS             complement(<1..161)
                     /locus_tag="KR314_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329425.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSA"
     gene            complement(179..526)
                     /locus_tag="KR314_003062"
     CDS             complement(179..526)
                     /locus_tag="KR314_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBH0329426.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            784..2166
                     /locus_tag="KR314_003063"
     CDS             784..2166
                     /locus_tag="KR314_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBH0329427.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            complement(2247..4040)
                     /locus_tag="KR314_003064"
     CDS             complement(2247..4040)
                     /locus_tag="KR314_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0329428.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILDELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(4293..5684)
                     /locus_tag="KR314_003065"
     CDS             complement(4293..5684)
                     /locus_tag="KR314_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBH0329429.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
BASE COUNT         1947 a         1041 c          695 g         2187 t
ORIGIN      
        1 gctgaagacc atatatcagc tctagaagaa tgaattatct ctttattatt atctaaaagg
       61 ttttgcaaaa ttttaaagct tatatctttg tattcttgat ttaaattgga gttgcaaaat
      121 ctatttatct tgtccttaat ttcttctatc ttgcctgtca tgactcctcc tccatatttt
      181 acatatataa aactagtgct acaaactcaa gatcttcttc tcctatattt tctatagaat
      241 gtttttcgcc atcagctgta tacactacat caccctcatt tacttctaca aattccccat
      301 tatcaaatac tttacctttt ccctttagta tataatatac ttcaaaatct gttgtatggt
      361 catgcacacc aattgaactt cctactggta aagctactct tttaaatagt cttccttttc
      421 ctttaagctc ttcattttgt attatgtttt caagcttaac ctttcctata ccaccattta
      481 aattttcttt tacttctact tgtaattcag atttctttct tatcatagct catcccacct
      541 gccaaacaat caatctaact actaaattgg catatccttt ccataaatat gtttgtattt
      601 taatataata ataacatata ttatcataaa aaaatattac tgaatacaca aaaatgatac
      661 tatttttaca tatatttcga cttaattaaa ttatcttaca agttgacgtc tatatgaaaa
      721 aaacatatac tcatatagta tggtaaaatt ttgttaaact atttatctgg aggttctcga
      781 catatgtcag ttttaaaaac gcaagaaaaa cttaaacaac ctagagattt atatttctgc
      841 tgttcaatat ttacctttgt aggacttgca tactatggta tgaaattggt attattattg
      901 tttttagctg aacaagtctc caaaggtggt ttagggttaa caccagcaga atccgcatca
      961 atgcttgcat cttttttagc atggacttat ttctctccag ttattggtgg atgggtcagt
     1021 gatagatttt taggtcccaa aaactgtatt atacttggaa tgctaatggt ttcaattggt
     1081 tattttctag gatacatagc aaatagtaag atggatataa acatcatgat atttttatct
     1141 ggattaggaa taggttttta taaaggaaac ttacatacta tggttggaaa cttatatacc
     1201 aatgatgacc caagaaaaga tggagctttc tctattacgt atatggctac taatctaggt
     1261 actttatttg gccctttaat atgcggttta gttgctaatg aatggtttgc tatgaaaagc
     1321 ggattatcta taagtatgta tggatataga tatgtatttt tattttcatc aattacggtt
     1381 ctaataggtt tattcttctt tatatttgga aacagaaagt tttataaacc tttgaataat
     1441 gaccctagta acgggagaga gtctataaga aaatcactag aatataagaa aatgctcgct
     1501 tcaagacctt taactttgat tgagaaaaaa agaataatag taatattggt gttagctttt
     1561 ttcacagtat ttttttggat agcatataac caagcctcta tgtctattgc actctataca
     1621 aaggaacaca taaatcttac tgttggcaat tttcaaattc ctacttcatg gatagattca
     1681 tacaatggtc tcctttgtgt tattcttgga cctatctctg ctttagtatg ggtaaaatta
     1741 tctcaaacta aaaaaggcga tttaagtgtg ccaaaaaaaa tgagcttagg ttttatacta
     1801 ttagctatag catttttgtt tatgatagta gcagttatac aaacaggaac agaccctaat
     1861 tcaattcaca aagcaagtgt attttgggta gttggatttt taacattcca aagtatagga
     1921 gaaatttgct tttctccagt cgggtatggt atggtaaata aactttcgcc tgaaaagtat
     1981 atttcattcc taatgggtgt ttggttttta ggcaaatttg cagcaaataa attatctggc
     2041 tatacgcaag ctattataga ccaacttggt atgttacaag tcttcatagt gataccatca
     2101 tttttattga tatttggaat tatactactt ataatgaata aaaaattagt agaattatca
     2161 aattaaaaat tttttaaagt taataaaata tacaataaaa aatcgaggca ttataatacc
     2221 tcgattttta ttgtatctag tacaaactat atagctctag tgtaaacttt taaccattct
     2281 ctttcttcat cagttaagaa aggtgatatt ttatcataaa ctaacttatg ataccagttt
     2341 aaataatctt tttcatcctt attcattaat tctggtacta taccatctaa gtcaatagga
     2401 gctaacgtaa caacttcaaa ttccataaat tgaccataga aattcttttc agcttttcta
     2461 actacaattt cattctctgt tcttatacca tgagaacctt ctatatatat acctggttca
     2521 ttagtagtta ccattccttc ttcaagaact gcactatcaa atctttcagg tactactctc
     2581 catctaaatc catttggagc ttcgtgaaca tttagcacaa atcctatacc atgtccagtt
     2641 ccacattgat aatcaattcc catattccac atgcaacttc tagatagtat gtctaagtta
     2701 tatcctctac aaccatgcaa gaacttagcc ttagaaagat ttatcattcc tcttgctaca
     2761 gaagtaaagt gtagttttaa ttcatcactt ataggtccta aaactgtagt cctagttata
     2821 tcagtagttc catcataata ttgtccacct gaatctacta aaaataatcc ttctgcctct
     2881 aatttataat ttgactctgg agttgcacta tagtgcatca ttgcagcatg ctctttatat
     2941 gcagcaattg tattgaaact tggctcaaag aaaccttctt gctctcttct taagtcctct
     3001 aatttttgag tagcacttat ttcagtgatt tccatcttac ctacattttt ctttaaccag
     3061 tacataaatt tagtgaaagc cacaccatct tttacatgtg aatttcttat attttctaat
     3121 tctacctcat ttttttgagc tttaaaaaac attactggat tgaactcatc aactttctca
     3181 acttcacaag gtatattatt gtaaattgta taactaagct tagttccatc taataaaact
     3241 ttttcagttt tgtctatatt ttttacaaat tcatacacat cattatatgg ctttatttga
     3301 acattttcct tagctagttc atctaatatt tcttcattta acttactttc atcaacaaat
     3361 aaataaactt cttttaaagt tattactgca taagaaagta caactggatt atatttaaca
     3421 tctccacctc ttatattgaa taaccatgct atatcatcaa gagtagttat tacatgagta
     3481 gaagtacctt tttcactcat tttttctctt aatctagcta attttgagct aaaactttct
     3541 ccacaatatt taacatctaa taaaaatgct ttactatctg ataaagcagg tctatctggc
     3601 catataccat ctattaaatc atactgatat tctatcttta tacctttttt agataatttt
     3661 tcagctaatg tagctccttc tctagcagat ataactctac catcaaatcc taaagttcct
     3721 ccttcaggaa tatttttata taaatattca tctgtagttg ggcaaccttc ttgtcccatt
     3781 ttgaataatt ttattgtact tccttctaat tgagattctg cctgtataaa atatcttcca
     3841 tcggtccata acccagcttc gtctttagtc acaataaccg tccctgctga accattaaac
     3901 cctgatataa attctcttga tttgaagtaa tctcctacat attcactttg gtgattatca
     3961 gaagatggaa tcatataggc atctatattt ttttcttcca taaattttct taatccactt
     4021 aatctatctt ttatattcac aacaagacct cctcgaaata atagtttgaa taactaaata
     4081 tataagtatg taaagtttat tacataaata ttttttgtat aatttatata aatcttatac
     4141 actctaatta tcacaattat aattttaatt ataatatcta tttgtgtaaa gctttttttc
     4201 ataatcaaag aggaaattac atttaatttt atatttaaat gtaacttcct ttatttgaag
     4261 taattcatct atttacaata attcgtcatg aattattttt cttttgttag taaatccata
     4321 gccattaatc ttgttaatac atttcttatt cccaataata ttaaaccaaa tattattaaa
     4381 actgttgcta ttcctccaaa tatctgtaaa aaaccaagtg tggctgtaaa accagcaatc
     4441 tttcctgata ataaacttgc aaaaaaactt gttagatacc aggctcccat agataatgta
     4501 gcatacttag ctggagctaa tttattgaac attgcaagtc ctataggaga taagaataat
     4561 tcacctactg ttgtaaatat ataagtcaat actataaaca atatactagc ctttaccatt
     4621 ccatctggac cattagctaa tgtagcaaat accataacta taaacccaac acccattatt
     4681 atcattccta atcccatttt aactggaata gagaaatcac ctcttttggt atgagataat
     4741 ttaacccata gggtagctaa tactgggcat aaagccacac ataatatagc atttagtgat
     4801 gtaagccatg ctactggcat ttcaaatcca aatagagttc tatttacatt atcccttaca
     4861 taaatcataa aagaactttg tgtttgatag tatgctgtcc agaataagac cacaaataaa
     4921 aataaaataa ccattgctat tatcctattt ttctccaact tagtaagtgg tccatactca
     4981 attttttcac ctttattacc tttagtcaca ggatattttc cgacttcatt taataatttt
     5041 ggtgcaagaa gtgtaaatat aatcatacct aacatcatgc ctataccaca aattaaaaat
     5101 gccatcttat atccataaga tactatatct ccacttgctc ctcttactgc aaatacatta
     5161 tcagaaagca atccacatat tattggagct ataaatgctc ctacatttac tgccatatag
     5221 aataaactgt atgctgcatc ttttcttgac acatcagttt taggatatat atccccaact
     5281 atatttgaac aacttgattt aaaaaaacca tttcctaaaa taattattga tagacctacc
     5341 caaaccatgg caatagatgt agatgaagca aatagtacga aatctcctat tgctattaaa
     5401 agtgcaccta tagaaataca tttttgttga cctaaaactt tatctgctaa ccagccaccg
     5461 actaatggtg taagatatat aagacctgta aataagccaa acatggatgt tgcttcttga
     5521 gtagaaagac ctaaaccacc ttgagctaca cttgctgtaa aaaataatac caataaagct
     5581 gttaaaccat aataactaaa tctttcccac aaaattgtca tgcaaataag ccaaaaactc
     5641 ttcggatgac cacttttttt agatacgctt tctgtacttg acataaaaaa acgccccctt
     5701 ttgttttata gttgaattta atattaatat tgctttaacc attttagcac atacttataa
     5761 tatcatcttt tctatgttta ctcaatagat tatcgcctgt attttcattt ttttattatt
     5821 atttttaaaa ttatgaaaaa tattattttt ttagtatctt cagtagaaaa
//
LOCUS       DAEPLF010000205         5856 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEPLF010000205 DAEPLF010000000
VERSION     DAEPLF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.206"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..120
                     /locus_tag="KR314_003066"
     CDS             <1..120
                     /locus_tag="KR314_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0329430.1"
                     /translation="DLSIREWICPSCNETHDRDINASINILKEGLRLITTQNK"
     gene            239..757
                     /locus_tag="KR314_003067"
                     /pseudo
     CDS             239..757
                     /locus_tag="KR314_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            784..2145
                     /gene="rlmD"
                     /locus_tag="KR314_003068"
     CDS             784..2145
                     /gene="rlmD"
                     /locus_tag="KR314_003068"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBH0329431.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMNEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            2668..3702
                     /locus_tag="KR314_003069"
     CDS             2668..3702
                     /locus_tag="KR314_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBH0329432.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPYEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKNKKRKKI"
     gene            3789..3992
                     /locus_tag="KR314_003070"
     CDS             3789..3992
                     /locus_tag="KR314_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBH0329433.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            4163..5323
                     /locus_tag="KR314_003071"
     CDS             4163..5323
                     /locus_tag="KR314_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBH0329434.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVIIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
BASE COUNT         2324 a          583 c         1128 g         1821 t
ORIGIN      
        1 gacttaagta taagagaatg gatttgtcct agttgtaatg aaactcacga tagagatata
       61 aatgcaagta taaatatatt aaaagaagga ttaagactaa taacaactca aaataaataa
      121 cttatgtaag aaccgtagga actacgggga tagcctgtgg agaattagta agacatattt
      181 tagtatgcaa aattctgtga agcaggaacc ctgtgacttt agtcatggga ggttcaggat
      241 gaataaacct gatggatata tatcagcaac tacagataaa tatgacccaa cagtattaga
      301 tttaattgat ttatcatact tagcttttga accatttcct gttggaaggt tagataaaga
      361 cacagaggga ttattagttt taactaatga tggaaagctt tctcatagag ttttatctcc
      421 taagaagcat gtgcctaaga cttattatgc aaaaattgat ggagtagtta ctgaagaaga
      481 tgtagaagct tttttagaag gtgtagtgct tgatgacgga tataaaacta tgccatcaca
      541 gttaaatata ttaaaaagtg atgatgaatc agaaatagag cttacaatac atgaaggtaa
      601 gtttcaccaa gtaaaaagaa tgtttgaaag cgtaggcaaa aaagtagtat acttaaaaag
      661 attgtctatg ggcaatttaa aattggatga aagtttagag ttaggtgagt atagagagtt
      721 aacagatgaa gaagtaaaat tgattgaaga aagataataa agatactata gtaaattgga
      781 ggaatgtctg tgaagaaaaa agatgttata gaatttgaag tagataaaat ggaatttggt
      841 ggtacatctt taagtcaagt tggagataga gttatccata tgaagggtgg aataagtggt
      901 caaaaggtga gagctggagt taaaaaagtt agaagtaaga aagctgaagt taagatgata
      961 gaattattag agaactcacc acttgaaact gaagaaactt gcaagcactt tagagagtgt
     1021 ggaggatgta cactattgtc tgtgccatat gaaaaacaat tagaaataaa agaaaaacaa
     1081 gttatggatt tatttttaaa gcaagattta tttggatttc agtttttagg gatagaagga
     1141 agtccagaaa acaaatatta tagaaataag atggaatata cttttggaga tgaagtaaaa
     1201 aatggacctt tgacattagg tcttcataaa aaaggaaagc atatagatat acaaactgtt
     1261 gaggagtgta tgttggttga tgaagacttc tcaaagatat tagtttctag tgtagagttt
     1321 ttcaatgaaa aaaagcttcc ttattataga acaatgaatc acaaaggata tttgagacat
     1381 cttgtagtta gaaaagggat tcatactaat gagattatgg ttaatatagt aacttcttct
     1441 caagaagatt ttgatatgaa tgagtttaaa gatatgcttt tagggattga tttaaaggct
     1501 gagttagtag gtgtacttca tactattaat gatggtttag cagatgcagt ccaatgtgat
     1561 gagttaagag tattgtatgg aagagattat atacaagaag aaattttagg tcttaagttt
     1621 aagatttcac cattttcgtt tttccaaacc aatactaagg gagctgaggt tctttatagt
     1681 attgctaggg attttattgg agattacaat gataaggttg tatttgactt gtatagtggt
     1741 actgggtcta ttggacaggt tatggctggt gctgctaaga aagtgtatgg tattgagata
     1801 gttgaggaag ctgttgttgc tgctaatgag aatgctaagt tgaatggact tactaattgt
     1861 gagtttattg ctggggatgt ggctaaggtt gttaaggatt taaaggacaa gcctgatttg
     1921 attattgttg accctcctag accaggcatt cataaggatg ctattaggga tatttgtgga
     1981 tttggtgcta aggagattgt ttatatttct tgtaatccta agtctttggt tgttgatttg
     2041 gttgatttta aggggtatgg ttatgagatt aagatggtta agtgtatgga tatgttccca
     2101 aatacgccac attgtgagac tgtagttaaa cttattagag aataacaggg tttcagactt
     2161 ttatattgtg tcttaaaagt agatttgcca cctatttgcc accataatta tttttgggtg
     2221 gcaaatttat tctacaatga tttcaaaaat atttaccatc tggtttctca tgttattagt
     2281 tacatgtaaa tatgtatcca tagttgttga agttttagca tgtccgagta tatgctggat
     2341 atcttttata ttagtaccat tttctaataa catagtatca gggtttttaa aataaagtgt
     2401 gtattcttct agagtagtga taattaaaat atcaaatatc actatacaaa ggaaagtgca
     2461 ccctttttat gtttagcaaa aacgaaatat taaaaaaact tattgaagaa tatgatgtca
     2521 aaacaactac agatattcaa gatatgctaa aagatttatt tacaaataca attcatcaaa
     2581 tatttccatt gtcgaatttt aacggtttaa acataatata ttgactgata taataaaatc
     2641 atagaatatt ttagggggaa atagattatg tttaagaaaa agttaatagc tgttgcaatg
     2701 agtataacta tgattagtgt agggagcttt tcgtatgctc attcaggaag aactgattca
     2761 tcaggaggtc atcgtgataa taaaaataaa tctggattag gaagttatca ctatcattgt
     2821 ggtggttatc cagcccattt acataataat ggtggttgtc cttatactgg aggaggaagc
     2881 agttctggaa caacaacttc ggttaataat gaagaaaagc aaaagaggtc tgttggagaa
     2941 aaaggatata atcaagggta tgaagatggg tataaaggaa attatagttc atcaaattat
     3001 agtggagatt atagtgatac atatgaatca aagtactcag aaggatatga aaaaggtaag
     3061 gctaaattag aagaggaaga aaaggtggct aaagagactg gatataattt agggcttaca
     3121 ggtgctaaat ctaataatac atatgaaaaa gaagttttaa aaaatgctta tgataccgga
     3181 tattcaactg gatacaatga atataagact aaaaagatag aagaatataa agctaaaggg
     3241 atagaggata gcaataaaga taaagaaaaa atgacatttg aagaaaacat agattctgaa
     3301 tttatagatg cttataataa tgcttatgac gaaatacaag aacaattaaa aaatgattac
     3361 actactcaag gatttgagag tgctatcaaa ggagaaagat ttgacacatc aactataggt
     3421 aatgttaaat atgctaattg gtttaaagaa ggatatgata atggtaaggt taagttacct
     3481 aaagcaaaag aaagtgtata taatcaaggt tacaatgaag aagattttag tataccatat
     3541 gaaatgaagt ctattgaaac taagttaaaa ggtgtatatg atgaaggttt agaaaaaaga
     3601 gaagaagaga aaagtagaaa tgctacttat ggggttggag ctggaacagc agctgtagtt
     3661 gctggagtag tatataaaaa taaaaaacgt aaaaagatat agatgctaga tataccaatg
     3721 atgacaagtg tataacaaat ataattttaa gtataaatat ttaaaatata gaagggaagg
     3781 ttatatctat gggatttaac tttaggaaaa gcataaatct aggaggagga ctcaaattaa
     3841 atttaggtaa gaagagtgta ggtataagtg ctggtgttaa aggtgctaga gtgtcagtta
     3901 attcaaaagg aagaaaatca gctacattga gtatacctgg aactggaata tcatatacaa
     3961 aaactagtac aaagaaacgt aaacctaaat aaatatttga tatactaaga aatagactgt
     4021 tacaaatagt ctatttttta gtatatagat ttaaaaagtg acaaaatttt gaaatataaa
     4081 ctaatttcat aaaggaaaat attcttttat gtagaattat aatatttaag taatattata
     4141 aaatttatag gaggatttca ttatgaaatt aagcaaaaca ctattggcaa tagggatgtc
     4201 tgtttcatta ttgattgcta gttgtccaac atcaaacgct cttagttcta ttaattcaat
     4261 aaaaggtaca aataagtatg aaacagcagg tttaatagca gataaacaaa attatgatat
     4321 tgcaatactt attaattctg aaaatggatt atctgatgga ttgtcagcaa gtggattgtc
     4381 aggagctaaa aatgccccaa tattgttaac taagaaagat acaatccctt cagaaacatt
     4441 aaaaagatta gaaaaggtaa aagaagtata tctaattgga ggaaaaaatt cgataggagc
     4501 atcagttcag aaaatattaa gtgataaaaa aataagcata aatagaataa gtggaactga
     4561 tagaatagat acaagtcata aagttgcaaa agaaatagga agagtaagta cggttaaaaa
     4621 agtaatatta actaatgctt ttaaaggcga accagatgct atgagtgttg cttctatagc
     4681 agttagagat aaatctccaa tagtattaac tgatggaaat actacaagtt ttgctacaga
     4741 tggattagaa acttatgcaa ttggtggaaa agtcagtatg agtgataagt taataaatga
     4801 aactaaagca acaagaatag gtggagtaga tagatacgat acaaataaac aaataataaa
     4861 taaattttat aatggctcaa aagaatttta catagcaagt gggactgatt tagtttattc
     4921 tttagttggt tctacaattg cacaaaaaac acctatagtg ttagttggaa atggaagtaa
     4981 taagagtata ttaaaaaatg caagtaaaat aactgctatt ggaaatattg atgatgttat
     5041 tataaaacaa tgtttaaata taactaataa tgttggggat tcaaatacag gtattgtaaa
     5101 agatactaat tcaaataata caaacaaacc aagtaatgag cctagctcta ataatcctgg
     5161 attaactgat aaaagtacag tatatgtaac tcctaatgga aaatcatatc atctaacaaa
     5221 aaattgttct acactaaaaa gaagtaaaac aataaataaa gtaacattaa aacaagctaa
     5281 gtcacaaggt aaaatagacc cttgtaatgt ttgtgtaaaa taatgtatta gccaaatata
     5341 tattaaaaaa gacaattaat taagattgtc ttttttattg aagaaatata aatttatatt
     5401 atgtattatt aaaaagaaaa ttattaatta taaaaaataa taataaagac gataagtatt
     5461 aaaattttat gtttccaata tttatgatat aattaatatg gcaaggaata aatatttgaa
     5521 aaagctatga gtggtgtttc catggaagtt tcttcacttt cttatgagag gaggtgaaaa
     5581 ctatggaaaa tctaatgatg agtattataa ctggtgttat agccagttac atttacgata
     5641 aattaaagag ccactctgac caatgaacta tacccactgt tttatacagt taaaacatgt
     5701 aaaagtggtt ataaagtact agtgtcaatc attagtactt tttttattat attcatataa
     5761 ttaatagcta tattttaaag tttactatct aaaaaatagc acgtcaaata aaaccctaga
     5821 tatttaaatt tttttagagc tacttaaaat aaatta
//
LOCUS       DAEPLF010000206         5846 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEPLF010000206 DAEPLF010000000
VERSION     DAEPLF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.207"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            113..2344
                     /gene="pflB"
                     /locus_tag="KR314_003072"
     CDS             113..2344
                     /gene="pflB"
                     /locus_tag="KR314_003072"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBH0329435.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFS
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            2504..3238
                     /gene="pflA"
                     /locus_tag="KR314_003073"
     CDS             2504..3238
                     /gene="pflA"
                     /locus_tag="KR314_003073"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBH0329436.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3360..5030)
                     /locus_tag="KR314_003074"
     CDS             complement(3360..5030)
                     /locus_tag="KR314_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBH0329437.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
BASE COUNT         2076 a          830 c          835 g         2105 t
ORIGIN      
        1 tattattata ttagaaaata tatttttaaa aaaatatata aaataaatta agtatataaa
       61 aaataatttt aatagtatta tttatcattt aaatctaagg aggaaatact taatgaatgc
      121 atggcaagga tttaaaacag gaagatggac taaagaaata aatgttagag agtttataca
      181 attaaactat tctccatatg agggaaatga ctctttctta gctggtgcca ctgaaaatac
      241 taaaaaatta tgggacgaag caatggtcct atttaaaaaa gaaagagaaa atggtggtac
      301 tttagatgtt gatacaaaaa ctgtttctgg aatagctgca tatgctccag gatatttaga
      361 taaagaatta gaaacaatag ttgggttaca aacggatgcc cctttaaaaa gagctgtcat
      421 gccatatggt ggtataaaga tggttgaaaa ttcatgcgaa gcttttggat atgaattaga
      481 cccagaaata aaagatatat ttactaaata cagaaaaaca cacaaccaag gagtttttga
      541 tgtttatact ccagaaatga gagctgctag aaaatctggt ataataacag gtcttccaga
      601 tgcttatggt agaggtagaa taattggtga ctacagaaga gttgctctat acggtgttga
      661 tgctttaata gaagataaaa atgagcaaaa gaaatcatta gaagtttctt gcatggatga
      721 ggaagttatc agattaagag aagaaataac tgagcaaata tcagctttaa atgaacttaa
      781 aaaaatggct gaatcttatg gatttgatat atcaaaacca gcaactaact caaaagaagc
      841 tgtacaatgg ttatactttg gttatttagg tgctgttaaa gaccaaaatg gtgctgcaat
      901 gtctttaggt agaacatcta ctttcttaga tatatacttt gaaagagatt taaaagctgg
      961 aatcgtaaca gaagaagaat tacaagagta tatggaccat tttgttatga aattaagaat
     1021 ggttaagttc ctaagaactc ctgattacaa taacctattc tctggagacc caacttgggt
     1081 aactgagtgt ataggaggta tgggaataga tggtagaaca ttagttacta aaaactcatt
     1141 tagaatgtta aatactctat atacattagg accttctcca gagccaaact taacagttct
     1201 atggtcaact aaattacctc aaggattcaa agatttctgt tctaaagtat ctatagatac
     1261 aagttcagta cagtatgaaa atgatgattt aatgagagca tactggggag atgactatgg
     1321 tatagcttgt tgtgtatctg caatgagaat tggtaaacaa atgcagttct ttggagctag
     1381 agttaactta gctaaaactt tattatacgc tataaatggt ggtgtagatg agaaatctgg
     1441 tgttcaagtt ggaccaagat ttgaacctat aacttctgag tacttagatt atgatgaagt
     1501 tatgagtaaa tttgaaccat tcactgattg gttagcaaca ttatatgtaa atactttaaa
     1561 tgtaatacat tatatgcatg ataaatattc ttatgaagca ttagaaatgg cattacatga
     1621 tagagatata tttagaacta tggcttgtgg tatggctggg ttatcagttt gtgcagactc
     1681 attatctgct ataaaacatg ctaaggttaa aactattaga aatgaacaag gaatagctgt
     1741 tgattttgaa atagaaggag attatccaaa atacggaaac aatgatgata gagttgatag
     1801 tatagctgta gagttagttg aaagctttat gaataaaata agaaaaaata aaacttatag
     1861 aaattcttat ccaactcaat ctatacttac aataacttca aatgttgttt atggtaagaa
     1921 aactggtaat acaccagatg gtagaagagc tggtgctcca tttgctcctg gtgctaaccc
     1981 aatgcatggt agagatacta atggtgcttt agcttcatta tcttcagttg ctaaattacc
     2041 atatgaacat gctcaagatg gtatatctaa tactttctct atagtacctg ctgctttagg
     2101 aaaagatatg actgaaagaa taaacaatct ttctgcaatg atggatggat acttctctca
     2161 aaatgctcat cacttaaatg taaatgtatt tgatagagct actttagaag atgctatgga
     2221 acatccagaa gaatatccac aattaactat aagagtatct ggatatgcag ttaacttcat
     2281 aaaattaact aaagagcaac aattagatgt tataaacaga acattccacg gtaaaatggc
     2341 ttagtcttta aatttttata ctttaactaa gattactact gtgtaaaaaa taaaaataaa
     2401 aggctatctt atatttgaat tatttctggt ataagatggc cttttttaga caaatattat
     2461 aactattgat aaggttatta aaatatattg tgaggtgaac tctatgatta agggaaagat
     2521 acattctata gaaacgtttg gtactgtaga tggaccagga ataagatata tattattttt
     2581 tcaggggtgt cctcttagat gcaaatactg tcataacagg gatacatggg atattaaatc
     2641 aggaaaagaa tacacagttg atgaaataat aacggatgct cttaagtata cttcttttat
     2701 gaagttttct ggtggaggta taactgcctc tggtggtgaa tccactcttc aaccagaatt
     2761 tttaagtgaa ttatttaaaa aagcaaaaga aaatgatata cacacttgct tggatacatc
     2821 tggattcgta gatatagaaa ctatagaccc agttttagat aatactgatt tggttttact
     2881 tgatttaaaa catatggttg aggaaaaatc tattgatttg actggtgttg gtatggataa
     2941 ggcattaaaa cttgctagac acttagaatc aagaaatatt cctgtatgga taagacatgt
     3001 tttagttcct ggcattactg atgatataga taatttagaa aaactaggtc aatttgtagc
     3061 tacattgaaa aatgtagaaa gatttgaatt gctaccatac cattctatgg gaatacacaa
     3121 atgggaaagt ttaggtatcg attatgaact aaaagatgtt cctgatgcca ctaaggaaga
     3181 tgtccaaaaa gctagtgaaa taatctctaa atttggagtg aaagtatata atagttagtg
     3241 tagtatatta atcctttata gaagacataa caaaataggg taaagtgtaa atttttaata
     3301 aacttcaaaa cttccacttt gccctatttt tatatactat atatctcatt ttatgtacat
     3361 cactttgata gttgaaattt ataaaattca gaggcactta caggtcttcc aaataaaaaa
     3421 ccctgaataa tatctacacc taagcttttt actatatcat actcttcata agactctatt
     3481 ccctcaacac aaacttttaa atttataata tgagctaatt taataatata ttcaagaaaa
     3541 gtatattcat aactattata tgtaatattc ttaacaaaac ttctttcaat ttttattata
     3601 ttaacagaca gttctttaag gtaatttaaa gatgaatacc ctgtaccaaa gtcatctatt
     3661 gcaatatata ctcctatacc ctttaaacta ataaatttat catttagtaa atttatatct
     3721 ggtatccaac aattctctgt taattccaaa attaaaaatt caggtcttaa ttgatactct
     3781 actaaacatt ctactataaa atctctaaaa aaatcttctt ttaactgaat atacgataca
     3841 tttacagata ttttaaagtc tggatttatt ttatgccatt ctttacactg ttttacagct
     3901 tcttttataa tccacttccc aactggtata attaaattac tctgttctag tattggtata
     3961 aattcaacag gagatacttc tccataagta ctcgaatgcc atcttaaaag tacctctgcc
     4021 cctattacct cttttgtaac agcatttacc tggggttgaa aaaacagctc aaagtcatta
     4081 aaattatttt caacgcactc tcttagtttt tgttgcattg aaattacctt tagtttattt
     4141 tcatagagct cttgtgaaaa aaacttaatt ctatttttac cactaatttt tgctatatcc
     4201 aatgcaatat cagcatgttt aaataaatct aaataattat ctccatcttc tggatacata
     4261 gcaactcctg cagtcactgt gcaatagtac ttatttgatt caatttcatg ctgagtattt
     4321 gtatacatct gtatcttttc atatattttc tctatagttt ctttagtaca catagggtaa
     4381 aaaaatgcaa attcatctcc atctaaccta tacaatctta catcttttgg aagatattct
     4441 aatacttcag tagcaattgc acgcaatact ttgtcaccat aagaatgtcc atatttttca
     4501 tttatatttt taaaattatc aatatccatg ataaacatag caccattttt ataaattttc
     4561 tcttttataa gataattcat tctatgttca aattgttctc tattccacgt tcctgtgata
     4621 gaatcaaatt tatttttttc accaatattc tttattctcc cgactagaaa tatagttttt
     4681 gggtcatccg acacataaac tttacctcta caagaaatcc atactatttc ttcatattta
     4741 ttaatcaacc tatattctaa attgtgctca cctttttttc catgtagtaa ttcttgtata
     4801 tcatctttcc atatttgaac atcatccgga taaacaattt tactccaaca attgactaaa
     4861 tcagattcta tctctttact taagttaaat tcatcaaaaa ttgctgaaga aattttaaat
     4921 ttattcctat ttatatccca aaaaaatata taatcttctg tactttgaga taaaagttca
     4981 aagaaaattt ctatatggtc taaacttaaa gagttcgatt cttctctcat cttttccccc
     5041 tacaatatta ctattagtgt agtttaatca acaaaatatt cttatttaga ttatactatt
     5101 ttactaattt ttttatctca tcaaacacat ttccccaact ttagttatct tatcaaatct
     5161 cttactctta actaagtttt tttgagattc ataaagtaat ttttctacaa tcattactgg
     5221 tgatttattt tcacctgcta aaacaccatg ttaaatcatt tattaaatca ttttctctta
     5281 aaagtccatt ttttattata taaagagttc tcagttcttt attctataaa gcttttctat
     5341 tactcttgaa tttgttttat tttttctcag cctattctct ttggatttgg tattgctttt
     5401 ctatatattt tggcaaagaa gtgcctcttc ctataatata agattttgat atttcttcat
     5461 catctataaa ttttttaata gggattatat cattttttat atttatttat ataaatattt
     5521 tttgctttta attttttatc tcttattatg tacttacatt taatttattc ttttcagtta
     5581 ataaaatagg tacattttta gtacatgaaa atagagttgc ttacagggta tctaggattg
     5641 atgtactatt tactagtatt acattttcag ccgattcaac ccctcttttg cttacataat
     5701 ttaagtatat ttttctggat ttttaaattt atgtacttta aaatggatat ccttattttc
     5761 attgatttta taagaattaa taaaaaatac gattacacta cagaattaga atgtcaatgt
     5821 ttataaatat aaaataatat ttttta
//
LOCUS       DAEPLF010000207         5822 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEPLF010000207 DAEPLF010000000
VERSION     DAEPLF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.208"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            201..1610
                     /locus_tag="KR314_003075"
     CDS             201..1610
                     /locus_tag="KR314_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBH0329438.1"
                     /translation="MIFSSLIFLFLFFPVTLICYYLANPKYKNVMLLLASIAFYAWGE
                     PKYIFLMILSIIVNYIFGIKVSGSKNKKMWVVLSVIYNISILAVFKYSNFFIDNINFL
                     FNTDITIPEVKLPLGISFFTFQIMSYVIDVYRNDAKVQRKLANLALYISLFPQLVAGP
                     IVRYQTVADSIEYREHNWDKFTQGVNRFVVGLGKKVILANQLGVIADGVFNKSAGDLS
                     RLEVWLGIVCYTLQIFYDFSGYSDMAIGLGKMFGFEFLENFNYPYISQTVSEFWRRWH
                     ISLGTWFKDYVYFPLGGSRVSKAKLYRNLFVVWFLTGMWHGASWTFIIWGLYYGILIS
                     VEKAFLEKFLYKMPRIVRHIYLLLIVMIGWVFFRVDDLPAAIKFIGIMFGAGNYPVIS
                     DAFNVFIIEYWYIIVGAILLSTPIVKVVKSLLIKKNKNTLNSTLYQVTNVVFIVSCMF
                     IVIIMLSSSTYNPFLYFRF"
     gene            1626..2828
                     /locus_tag="KR314_003076"
     CDS             1626..2828
                     /locus_tag="KR314_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329439.1"
                     /translation="MSKKKPRIVAISFLIIVFGFFILNFTLPKKKLSITENRNLAKFP
                     TVENLKKCDFIAKFEEYFNDHFAFRDEMISINTRTQANLNKTKVGNYYLGKDNWILGM
                     FPEILDQKQLSRYEKSINYLSNLSKDMGKNVYFTMMPHKTNMLKHLYPKYVDNMDNID
                     INLKNFKNRLNPDLIKYIDMDEYFLSNYSDKERENLYFKTDHHWTGYGAFEGFKMMAS
                     NMDLGLSKQDLDKHFSSYKKSIITDKKFIGSYNQNIDMLIKENEYVVYMTKDNQDYSF
                     DINGEEKEEKEEKEVYATERDNKEWDYGGAYIRGAKTNILTIKNPKALVDKKILIFRD
                     SYQAPTTLLFADMFKEVEIADPRNINSIYKTYKEIIEESNADIVMFMYNSSGFDSMID
                     SMIDKGIK"
     gene            3029..4012
                     /locus_tag="KR314_003077"
     CDS             3029..4012
                     /locus_tag="KR314_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329440.1"
                     /translation="MKEVFNVHIMQSQNKKIKQKYYLTKDGWLLGNFAKLLSDYHIKS
                     YSKAINNLSKLNRKKDFYFVSLPHKTNMLKHLYPSGIEGKNNIDINKENLEKQLTDIN
                     FLDIDDYFYRSFTEKEREKFFLKTDHHGNSLGAFEEYKFILDKLDLGLSKSEIDSHLK
                     RYSTRYVNDKVFIGSYNKKMGYPLKKKESISYVEISDANYKYFISNGTKNTEINENII
                     KAKLKDKREWDYGGAYMRGCDCHILKIRNSSSLTNKKALIFRDSYQAPMTWMLADTFK
                     ELEILDPRYIKNIDMTYDEIIKNSNANVVLFMYNSFGFAGMIQEMIDKGIA"
     gene            complement(4462..4905)
                     /locus_tag="KR314_003078"
     CDS             complement(4462..4905)
                     /locus_tag="KR314_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329441.1"
                     /translation="MKKNMLYLGIVYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GICMIFQYMYWSKPERAVDYEEKIKNQRIEMNDERRVMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(5064..>5822)
                     /locus_tag="KR314_003079"
     CDS             complement(5064..>5822)
                     /locus_tag="KR314_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0329442.1"
                     /translation="IMYCGQYIKLPKLDMVKIRDKQVPKGRILNATISKEPSGRYYVS
                     LCCTDVDIEAFENTNNQIGLDLGIKEFCISSCGEFIENPKYFKKSLNKLAKLQRELSR
                     KTIGSLNRNKARLKVAGLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIKNR
                     KLSRLISDVSWSEFIHQLKYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIR
                     EWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         2222 a          742 c          796 g         2062 t
ORIGIN      
        1 ataatagctt tattaattca ttttaatatt acatcatttt aaaaaaatta tatgaaaagt
       61 caaaatttac atatagatta attaaacttg taaaaacaca tataaattca tacaattaag
      121 tacttaaaac ttgaaaatac ttactttgct atatatttta gataaagata taattgattt
      181 ggttaagaaa ggagtctaat atgatattca gtagtttaat atttttattt ttattctttc
      241 cagtcacact catttgctat tacttggcaa atcctaagta taaaaatgtt atgttacttc
      301 tagcaagtat tgcattctac gcatggggag aaccaaaata tatttttttg atgatactat
      361 caataatagt aaactatata tttggtatta aagtttctgg cagtaaaaat aaaaaaatgt
      421 gggttgtgct ttctgttatt tacaatatat ctattcttgc tgtatttaag tatagcaatt
      481 tttttataga taatattaat tttttattca atacagatat aactatacct gaggttaaat
      541 tacctttagg tatctcattt ttcacattcc aaataatgag ctatgtaata gatgtatata
      601 gaaatgatgc taaagttcaa agaaaattag ctaatttagc attatacata agtttattcc
      661 ctcagcttgt agcaggtcct attgttagat atcagactgt tgcagatagc attgagtata
      721 gagaacacaa ttgggacaaa tttactcaag gtgtaaacag atttgttgtt ggtcttggaa
      781 aaaaagtaat tctagcaaat caattaggtg taatcgcaga tggtgtattt aataagtcag
      841 ctggagattt atcaagactt gaggtatggc taggtatagt ttgctataca cttcaaatat
      901 tctatgactt tagtggctat agtgatatgg ccattggttt aggtaagatg tttggatttg
      961 agtttttaga aaatttcaac tatccatata tctctcaaac agtatctgag ttttggagaa
     1021 gatggcatat atctttaggt acttggttta aagactatgt gtacttccca ttaggtggaa
     1081 gtagagtctc taaggcaaaa ctatatagaa atctatttgt agtatggttt ttaaccggga
     1141 tgtggcatgg agctagttgg acttttatta tatggggtct atattatgga atattaatat
     1201 cagtagaaaa agcattctta gaaaaatttc tatataagat gccaagaatt gtaagacaca
     1261 tatatctact tcttatagtc atgattggat gggtattttt tagagttgat gatttaccag
     1321 cagctattaa atttataggt attatgtttg gtgctggaaa ttatccggtt atcagtgatg
     1381 cattcaatgt atttataatt gaatattggt atataattgt tggggcaatc cttctatcaa
     1441 ctcctattgt aaaagttgta aaatcattat taattaagaa aaataaaaat acattaaata
     1501 gcacattata tcaagttact aatgtggttt tcattgtttc ttgtatgttt attgtaataa
     1561 taatgttaag tagctcaacc tataatccat tcttatattt tagattctaa ttgggaggta
     1621 taaacatgag taaaaaaaaa ccaaggattg ttgccatttc atttttaatt atagtatttg
     1681 gatttttcat actaaacttc acacttccaa agaaaaaatt aagtataact gaaaatagaa
     1741 atcttgcaaa atttccgact gtagaaaatt taaaaaaatg tgactttata gccaagtttg
     1801 aagaatattt taatgaccat tttgcattta gagatgaaat gatatcaata aatacaagga
     1861 ctcaggctaa tttaaataaa actaaggtag gtaactatta tctaggaaaa gataactgga
     1921 tattaggtat gtttccagaa attttagacc aaaaacaatt aagtcgatac gaaaaatcca
     1981 ttaattattt atctaatcta agtaaagata tgggaaaaaa tgtgtatttt acaatgatgc
     2041 cacataaaac taatatgtta aaacatttgt atcctaaata tgttgataat atggataata
     2101 tagacataaa tttaaagaat tttaaaaata gattaaatcc agatttaatt aaatatatag
     2161 atatggatga gtatttttta agtaactatt cagataaaga aagagaaaat ttatatttta
     2221 aaactgacca tcattggact ggatatggag catttgaagg ctttaaaatg atggcatcaa
     2281 atatggactt aggtctatct aagcaagatt tagacaagca ttttagtagc tataaaaagt
     2341 ctataattac tgataagaaa tttataggaa gttataatca aaatatagat atgctaatta
     2401 aagaaaatga atatgtagtt tatatgacaa aagataacca agattatagc tttgatataa
     2461 atggagaaga aaaagaagaa aaagaagaaa aagaagttta tgcaactgaa agagataata
     2521 aagagtggga ttatggtggt gcatacatta gaggagctaa aactaatata ttaactataa
     2581 aaaaccctaa agcattagta gataaaaaaa tactaatatt tagagattca tatcaggctc
     2641 ctacaacact tttatttgct gatatgttta aagaagtaga gatagcagac cctagaaata
     2701 taaactcaat atataaaaca tacaaagaga taatcgagga aagtaatgca gatattgtaa
     2761 tgtttatgta taatagttct gggtttgatt caatgataga ttcaatgata gataaaggaa
     2821 taaagtagta aataaaaagc ctctattaaa ttcaaaattt aatagaggct ttttattgtt
     2881 tattttttat tgctttggta aaaataaact aatcaaaata aaagtaagga gatgtagcat
     2941 attgaacaaa agacatagtc tacctatagt atcagtgata ttattgatgg tcttaattat
     3001 ctttaataat aaccaaaatt atattacaat gaaagaagtt tttaatgtcc atattatgca
     3061 gagtcaaaat aaaaagatta aacaaaagta ttatctaact aaagatggat ggctattagg
     3121 caactttgct aaattattat ctgattatca tataaaatct tactccaaag ccattaataa
     3181 cttgagtaag ttaaatagaa aaaaagattt ttactttgtt tcacttcctc ataagacaaa
     3241 tatgcttaag cacttgtatc caagtggtat agaaggtaaa aataatatag atattaacaa
     3301 agaaaactta gaaaagcagt taacagacat aaatttctta gatatagatg attactttta
     3361 tagaagtttt accgaaaaag agagagaaaa attcttttta aaaacagacc atcacggaaa
     3421 ttcattagga gcatttgaag aatataagtt tattcttgat aaattagacc ttggcttatc
     3481 taaatcagaa atagatagtc atttaaaaag atacagtact cgatatgtaa atgacaaggt
     3541 ctttataggt agttataata aaaagatggg atatccactt aagaaaaaag agtctataag
     3601 ctatgttgaa ataagtgatg caaattacaa atattttatt agtaatggta ctaaaaatac
     3661 tgagattaat gaaaatatca taaaagcaaa attaaaagat aaaagagaat gggactatgg
     3721 tggagcttat atgagaggtt gtgattgtca catacttaag attagaaatt caagctcttt
     3781 aactaataaa aaggccctta tatttagaga ttcgtaccaa gcacctatga cttggatgtt
     3841 ggctgatact tttaaggaac tcgaaatact agatcctaga tatataaaaa atatagatat
     3901 gacctatgat gagataatta aaaacagtaa tgcgaatgta gttttattta tgtataatag
     3961 ctttggattt gctggaatga tacaagaaat gattgacaag ggaatagcat aacaatttga
     4021 atcagctcct ttttatacaa aaaatagttc tcaataattc ctaatctata tgataattta
     4081 gtacatctct ttaacaataa aaataaaatc acaaaaattc ttataccatc acttatcttt
     4141 atgataaatt ggttgagaaa aaattttata atttatatga tatgcaatct taattaattc
     4201 tttatctact gcaaccttta ataaaagaaa ccatccaaga ctattaatat gaatttatat
     4261 aaaaattact ttcaaataaa gtactgcagt atttttattt aaagtatcta taatctttaa
     4321 ccacaaaaag ccacaaaata caatgtatac ttatggcttt ttataaattt atataattgt
     4381 atataaagat atattctaaa actgtcttaa gaatctaaag tttaaaaatc caatattttt
     4441 attgtgaatt taaacttttt attacaactt tttatctaat ttattgtata caacaacact
     4501 acaaataaat tgaaataaaa taagcaatcc aagaacaact aaaacccact tgcttattac
     4561 cattacagaa aatacactca atatagaaac aataaatata agaattacaa gaacatagga
     4621 catgatttga ttagtgatac gtccagcttt atcacgtaac ataactcttc tttcatcatt
     4681 catctctatc ctttggtttt ttatcttttc ttcataatct actgctcttt caggcttact
     4741 ccaatacata tattgaaaaa tcatacatat acctggacct aaagcagctc cagataatcc
     4801 ccaaataaaa gcctcaaatg aaaactcagt aaacgtagcc aataaaacaa gtaaaattcc
     4861 acaaataaaa tatactattc caagatataa catattcttt ttcattttta ctcctcctca
     4921 taaataaata tttcttcaat actcatctga acctcccatg actaaagtca cagggttcct
     4981 gcttcacaga attttgcata ctaaaatatg tcttactaat tctccacagg ccatccccgt
     5041 agttcctacg gttcttacat acgttattta ttttgaattg ttattaatct taatccttct
     5101 tttaatatat ttatacttgc atttatatct ctatcatgag tttcattaca actaggacaa
     5161 atccattctc ttatatttaa gtccttaact tcctcgtttt tatacccaca tttattgcat
     5221 atttgtgaac ttgcaaagaa tttgcctact tttacaattt gtctaccata ccaattagcc
     5281 ttgtatttta attgatgaat aaattctgac catgatacat cagaaattaa gcgagatagt
     5341 ttacgatttt taatcatatt ctttacttgt aaatcttcta tgcaaataat atcattctct
     5401 tttattaatt tagtagatag tttttgtaga aaatcatttc tttgattagc tatatgttct
     5461 tgaagtcctg caacttttaa tcttgcttta tttctattta aactaccgat tgtttttctc
     5521 gataattctc tttgtaattt agcaagtttg tttaatgact tcttaaaata cttaggattt
     5581 tctataaatt ctccacaact tgagatacaa aattctttaa ttcccaaatc taaacctatt
     5641 tgattgttag tattttcaaa tgcttcaatg tctacatcag tacagcataa tgatacataa
     5701 tatctaccac ttggttcttt agatatagta gcattaagta ttctcccttt aggtacttgt
     5761 ttatccctta tcttaaccat atcaagttta ggtagtttta tatattgacc acaatacata
     5821 at
//
LOCUS       DAEPLF010000208         5797 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEPLF010000208 DAEPLF010000000
VERSION     DAEPLF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.209"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            104..3535
                     /locus_tag="KR314_003080"
     CDS             104..3535
                     /locus_tag="KR314_003080"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBH0329443.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            3777..5705
                     /locus_tag="KR314_003081"
     CDS             3777..5705
                     /locus_tag="KR314_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBH0329444.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         2459 a          611 c         1095 g         1632 t
ORIGIN      
        1 tttatcttat taattaagga actttcttaa acatcttcaa aaataaagta ttttagtcta
       61 taatctttaa tattacacaa acaataaagg agaagaatag agtatgctta aaaagtttaa
      121 taaaatacta gtggctaata gaggcgaaat agctataaga atatttagag cctgttcaga
      181 attggggata aaaagtgttg gtatatacag taaggaagat aagtatggat tgtttagaac
      241 aaaagcagat gaatcatatt tgataggaga aggtaaaggg ccaatagatg catatttaga
      301 tatggatgga ataatagacc tagcaaagag aaaaaaagtt gatgctatac atccaggata
      361 tggttttcta gcggagaatg cagaatttgc tcgtaaatgt gaagagaatg gaattacttt
      421 tataggacca tcatctaagg taatgaatat gatgggggat aagattaact ctaaaaaaat
      481 cgcaaaagaa gttaatgttc aaactatacc aggagtagaa aaagcaataa gaagtactga
      541 agaagcaaag gaagttgcaa acaaaatagg atatcctgtt atgataaaag catctaatgg
      601 cggtggcggt agaggtatga gaattgtaca tcgcgaagaa gacttagaac ttgaatatga
      661 aactgcttgt agtgaatcaa gaaaagcttt tggagaagat attatattta tagaaaaata
      721 tatagctgat ccaaaacata tagaagttca aatattaggt gataattatg gaaatatagt
      781 tcacttatat gaaagagatt gttcagttca aagaagacat caaaaaataa tagaatatgc
      841 accagctttt tcgttagatg ataaagtaag aaaagagata tgtgaagatg cagttaagtt
      901 atcaaaacat gtgggttatt ctaatgcggg aacattagaa tttttagtag atgcaaatgg
      961 tgggcattat tttatagaga tgaacacaag agtacaggtt gaacatactg taacagagat
     1021 ggttacagga atagatatag ttcaaagtca aatactaata gcccaaggtt acagcttaga
     1081 tagtgaagaa attaatataa aatcacaaga tgatgtggaa ataagaggtt actctatcca
     1141 atgtagaata actacagaag accctaagaa taaattcatg cctgatacag gtaaaattca
     1201 agtttataga actggttcag gatttggtat aagactagat ggaggaaatg gatttacagg
     1261 ggcaaacata agtcctcatt atgatagttt attggttaag acaatatctt gggatagaac
     1321 attccaagga gcaataaata aaactataag atctataaaa gaacttagag ttagaggtgt
     1381 taaaactaat gtaggattcc tagttaatgt tttaaataat cctatattct caaatggtaa
     1441 atgtagtact aagtttatag atgaaaatcc agatttattt gaaataacag aaagtaaaga
     1501 cagaggaaca aaattacttc aatttatagg agatgtaatt gtaaatgata atgcttgtaa
     1561 agaaaaacca ctatttgatg cattacatga cccaagaatg gataaggatg gttcaaaatc
     1621 agaaggaagt aaaatacttt tcgataagtt aggtaaatct gcatatatag aaaagataaa
     1681 aaatgataag aaattactat taacagatac aacaatgaga gatgcacatc aatcattatt
     1741 agcaacaaga ataagaacat atgacttatt aaaagctgct aagccaacag aaaaatatca
     1801 aaaggattta ttctcacttg aaatgtgggg aggagctact tatgatgttg catatagatt
     1861 tttaaaagaa tctccttggc gtagattaca aaagctaaga gaagaaatac caagcataat
     1921 gttccaaatg ttgcttagag catcaaatgg agttggatat aaaaactatc cagataatgt
     1981 aatagaagaa tttacaaaag aatctgctag acaaggtata gatgtattta gaatatttga
     2041 ttcattaaac tgggttgaaa atatgaaacc atctattaat acagctttag aaactggaaa
     2101 aattgttgaa gcaactatgt gttatacagg tgatatacta gataagacta aaactaaata
     2161 caatctagaa tattatatta aaatggcaca agaattagaa agcttgggag cagatataat
     2221 agcaataaaa gatatgtcag gtcttcttaa accatattca gcttacacac tagttaaaga
     2281 gttaaagaaa aatgtaaaag caccaataca tttacacact catgatacaa gtggaaatgg
     2341 tgtagcaact tgtttaatgg cgtcagaagc tggagtagac ataattgatg ctgcattaga
     2401 atctatggca ggacttacaa gtcagccatc gcttaatgct atagtagagg cacttaagaa
     2461 tacagagaga gatacaggaa tagatttatt tggatatgat gagttaggaa aatattataa
     2521 ggatttaaga aaagtttata ataaatttga aagtgattta actaattctt gtgcagaaat
     2581 atataatttt gaaataccag gaggccaata tacaaactta aaacctcaag ctgatagttt
     2641 aggattagtt aatagatttg atgaagttaa agaaaaatat aaagaagcta atgaagttgt
     2701 aggagatata ataaaagtaa ctccatcttc aaaagttgta ggagacttag ctatctttat
     2761 gactaaaaat aaattagata aagacaatat aatagaagaa ggtaaaaatt tatcattccc
     2821 agattctgtt gtggattact gtaaaggtat gattggtcaa ccagaaggtg gaataccaaa
     2881 agatttacaa gaggtagttc taaaaggtga agaagctata acagtaagac caggttcatt
     2941 attaccagca gaagattttg atgaaatagc taaatatcta aatgaaaagt atgatataaa
     3001 tgctaatata agaaatgtta taagttatgc tttatatcca aaagtatatg aagattatat
     3061 caaacatcta caacattata atgatatatc taaacttgaa agtgatgtat tcttctatgg
     3121 attaaataaa aatgaagaat gtgaagtaga aatagaagaa ggtaaggtat taacaataag
     3181 attagttgaa attggtgaag ttaaggaaaa tggatttaga actataggat ttgagttaaa
     3241 tggaatggtt agagaagtag aaataaaaga taagaatttc tctggaaaaa ttaataatgt
     3301 tgaaaaagct gatatgaatg accctcttca aattggagca agcataccag gaaaagttat
     3361 aaaaataatg gttaaagaag aagatgaagt aaaagcaaat caaccattaa tcgttataga
     3421 ggctatgaag atggaaacta taatagttgc taagactgat ggagtaatta agtcaatcaa
     3481 agtaaaagaa gatgatatgg ttgaagataa acaattatta atgataatga agtaatttaa
     3541 ttaaaataaa ttaaaaagca ctctgaaatg tttaataaaa aatgttttag agtgttttta
     3601 tttattttaa ttaaatattt gttaaaaaaa agaggacaat tacctccaga tgtagaaata
     3661 aagctagtaa ggaaaatgtt atctaaaaat tctaaatata gtaaatattt tccataatat
     3721 ttcttaggaa ataacagcaa agataaaata cataaattta aaaggtgggt gcttatatga
     3781 aggtttatga tagtaaaatg ttgagaaatg tagcagtatt aggacatagc ggatgtggaa
     3841 aaacaaatct aatcgaaact atagcttaca cagcaaacac taataagata cctaaattaa
     3901 cagacaaggt aaatatgact tatagcatgg gattaatacc tatagagtat aatgattata
     3961 agtttaactt attggatacc ccaggatatt ttgactttag cggagacgtt gtgtcatctc
     4021 ttagagcaag tgatgcagca ataatagtaa tagatgcaac agcgcctatt caagttggta
     4081 cagagaaatc attagaacta actgagagta ttcctaagat aatgtttata aataaaatag
     4141 ataatgaaaa ggcaagatac aaagatgcaa tagcaatgtt aagagaaaaa tacaataata
     4201 agatagttcc tatgataagt cctatataca aagataaaaa ctttgtaaag ttacataatg
     4261 tttttgaaaa catagatgat ttagaaggtg aatttaagga acaagctatg agtgttaaag
     4321 aagcattaat ggaacttata gcagaaactg atgaccaaat attagataaa tattttaatg
     4381 gagaagaact aactacggaa gagatacaaa aaggtattat tataggtata caaagaggtg
     4441 atattatacc tgtaatatgt ggttcaacta taaataatat aggcacaaag gaaatattag
     4501 atacaatatc aagttattta gaacctatct ttacagagga aagcaaacca tttaggggat
     4561 tagtatttaa aactatggtt gacccatttg ttggaaagat gtcatatata aagattactg
     4621 agggtgtact ttctaaggat aaagatgttt ttaatataaa taaaaatgta aaagaaaaaa
     4681 tagctaatat atatacattg agaaatagtg aattagtaga aatagaaaaa gctaaagctg
     4741 gtgatatagt agttataact aaagttaatt ctctaaaaac aggagacact atatctgcag
     4801 ataaagatgc agaagcttta gaaaagatag attttcctaa accacaaata tattatgcag
     4861 taactcctaa gaataaaggt gatgaagaaa aagtagcatc tgtattaaat aaactagtag
     4921 aagaggaccc tacattacat tggtatagaa atacagagac aaaacaagca cttttaggag
     4981 gtcaaggaga acttcatata aaaacaatta aaaataaaat gaaagataag tttggtgttg
     5041 atgttgaatt gaatgacctt aaagttcctt atagagaaac tataaaagga acagccgatg
     5101 tacaaggaaa acataaaaag caatcaggtg gtcatggtca atatggagac gtaaaaataa
     5161 gatttgaaag atgtgaatct gattttgaat ttacagaaga aatatttggt ggttctgtac
     5221 caaaacaata tataccagca gttgaaaaag gtctaaaaga ctctatgcaa aaaggtatat
     5281 tagcaggtta tcctgttaca aacataaaag ctacgcttta tgatggttca taccatgatg
     5341 tagactcttc tgaaatggcc tttaagatgg cagcaagtgc agcctttaaa aaaggtatgg
     5401 aagaagcgca cccaatacta ctagaaccta taatgaaact taagataact gttccagaag
     5461 aatatatggg agatgtcatg ggtgatataa ataagagaag aggaaaaata tttggaatgg
     5521 aaccagatga caaagggaaa caaataatat ttgcagaagc tccacaagca gagacattta
     5581 aatatgcaat agatttaaga gcaatgacac aaggtagagg atactttgaa atggaattag
     5641 agagatatgg agaggttcca tcacagtttg cagaaaaaat aattgggtta gctactgcaa
     5701 agtaatgaac aaaataaata gaaaaataat taatatatat tattaaaaat atttttaggg
     5761 ttttaactag gtataggttt atgttaggta aacttat
//
LOCUS       DAEPLF010000209         5666 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEPLF010000209 DAEPLF010000000
VERSION     DAEPLF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.210"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            395..1819
                     /locus_tag="KR314_003082"
     CDS             395..1819
                     /locus_tag="KR314_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBH0329445.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDALFKRLESK"
     gene            1854..3983
                     /locus_tag="KR314_003083"
     CDS             1854..3983
                     /locus_tag="KR314_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBH0329446.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDK"
     gene            4013..4435
                     /locus_tag="KR314_003084"
     CDS             4013..4435
                     /locus_tag="KR314_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0329447.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4454..5605
                     /locus_tag="KR314_003085"
     CDS             4454..5605
                     /locus_tag="KR314_003085"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH0329448.1"
                     /translation="MKKAIQFGAGNIGRGFIGSLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEEILNNIEKSYIFMKNL"
BASE COUNT         2353 a          584 c         1020 g         1709 t
ORIGIN      
        1 atttaaaatt aatctttagt gattcatata attacatttg tatagtttat attgataata
       61 ttactagaga gtgcataaac ctcaaaaata cagataaatt aaatctatat tagtatatcc
      121 aaaagtacat ttaataaatt tatttaactg aattattatt ttcatttcta ttaaatttaa
      181 atattttata aaaatagtat tatttacaaa atcatatact ccaaaatact acataaataa
      241 aattaaacaa aaactaaaat gacaaatgtc attatctaaa gtgacataga aacggtttct
      301 taaagttgtt actataaaat ataaactcta taataaaagt ataacaaaag ggaatcatat
      361 cgatatagaa tataattcaa gaggaggtaa aattatgagt agttctaatc aatcgacaat
      421 aaagacatct atacagcgag tagggaaatt tttaagtggt atggtaatgc caaatatagg
      481 agcttttata gcatggggtc ttataacagc attatttata ccaacaggat ggatgcctaa
      541 tgaaaaattg agtactatag gcgacccaat gataaaatac cttttaccac tattgatagc
      601 ttatactggt ggtaaagcaa tagcaggaca aagaggtggc gtaataggtg cagcagcagc
      661 tatgggagta attgttggtg cagatatacc aatgtttatc ggagcaatga taatgggacc
      721 atttgcagga tgggtaatta agaagtttga taaatttgta gatggaaaga taccaacagg
      781 atttgaaatg ttagtaaata acttttcaat aggtataata ggtatgcttc ttgcaatact
      841 aggattttat gcaattggac cagcaatagt agcagggaca gctcttatag aatctggagt
      901 tcaatttata gttagcaaaa gtttattgcc tctagtttca gtatttatag aacctggaaa
      961 agtattattc ttaaacaatg caataaatca tggtatatta gggcctatag gtatagctga
     1021 ggcaaaagaa gcaggtaaat ctataatgtt cttactagaa agtaaccctg gaccaggttt
     1081 aggagttcta ttagcatatt ggatgtttag tagaggaagt gtaaaacaat cagcaccagg
     1141 agctgtaata atacatttct ttggtggtat ccatgaaatt tacttcccat atattttaat
     1201 gaatccagtt ttaatattgg ctacaatggc aggtggagca gcaggaatat tgactttttc
     1261 aatattagga gctgggcttg tagcagcacc atcaccagga agtatatttg cacttatggc
     1321 actagctcca aagggaggat tacttccagt attagcagga gttgcagtgg caacagttgt
     1381 atcattctta gtggcagcac catttgttaa gagagcttca gcaaatcaaa gtgaagaaga
     1441 cagtacttct ttagaagaag ctaaagcaaa aatgagtgat atgaaatctg caagtaagaa
     1501 ctctgagaaa aatattgagg aaaaacagct tgaagtaaat gagattaaga agattgtatt
     1561 tgcatgtgat gcaggaatgg gttcgagtgc aatgggagct tccagattta aaaatagaat
     1621 aaaaaactta gacttaaata tagaaattac aaattcatct gttgataatc ttccagatga
     1681 tacacaaata gtagtaactc acaatacatt agttgaaaga gttgctaaga ataattcaag
     1741 tgttgagata gtttcaataa acaatttttt aaatgaccca aatttagatg cattatttaa
     1801 aagattagaa tctaaataaa aataaaaagt accactaatt aggagtggat aatttgaaaa
     1861 aaaagaagat tacatctagg caaaaaaaga taatactaat gatagtagaa aattcaaaga
     1921 aaaacattcc aataactatt tctgagattg cagggacttt agagctaagt tcaagaactg
     1981 tacttagaga tatgtctgga attgaaaaat ggtttgatga aaatgatttt aactttgtaa
     2041 aaaaaccagg agtgggattg attttagagg aaaatataga aaatcaaaac tttataattg
     2101 agttgttaga agaagaaaaa atagaaaaag aatacagcaa ggaagaaaga aatttaatta
     2161 tattatcaaa gctactagtt tctaacgagc cagtaaaatc ctattatttt gcaaaaatat
     2221 taaaggtatc tgaaggggta ctgaataatg attttgcctt agcaagtaaa tggttggaaa
     2281 gatttgatat tgagcttgtt agaaaaccag ggcttggtgt ctacttaaaa ggtcaagaaa
     2341 aaaactttag agaggcatat gtaaatctta tatacgattc attcaatgaa aaagaaatac
     2401 tagatatggt cagaaatata agtgaaaata tacaaacaga taaagcaata gaaattttaa
     2461 gtgaaaatag acttttaaat ttaatggaca ggtgcataat aagaaaagtt gaagctacat
     2521 taacaaagaa gctttcagat ttagatgtaa atttagctga tagtgcatat atagggcttg
     2581 tagttcatat ttcattagcc ttacagagga taaaaaatgg tgaaaatata actatggata
     2641 aagagtttct aaaagaatta tctataacag aagagtttaa attagctaaa gaaatcgtaa
     2701 aaggtatgga aacagatttt aacatggata taccagtaga tgaggttgga tatataacta
     2761 tgcatataag aggtgcaaag caaaggtcat cttcaaacca taaggcttta aacttagatg
     2821 atatagaaat aatggaaatt acaaataaga tgatagattt ggcggaagat gaatttaaaa
     2881 taagtttaaa aaatgacgag aggttattta aagacctagc aaatcactta gggccttcta
     2941 taaatagatt gaatatggga ttagaaataa gaaatcctct tttagatgaa ataaaaagca
     3001 agtattcata tgcctatgat ggagttgaaa agatttctag aattataaaa gataaactaa
     3061 atataaattc aatacctgaa tcagaaatag gatatattgc aatgcacttt gcatcagcca
     3121 tagaaaaaaa tcttatgatg aatactaata taaatattgt tgtggcatgt cccactggaa
     3181 taggtacttc aagattctta tcaactaaga ttgaaaataa atttcctaat ttaaatatac
     3241 ttgaaaccat atcagctata aacatagatg aagagtattt aaaagaaaaa gatgttgatt
     3301 taatagtgtc tacagttgag ttaaatacaa gtttaaatta tatatgtgta ggtccattta
     3361 tgagtttaga tgatgaacag ataatcaagg aaaaaataaa atctatagct caaaataaat
     3421 taatcaattt aaatgcgaaa aatgatacta aaagtaagaa taaagtatat gaacagataa
     3481 cagaaagtat gaatatagga aaagatattc ttcaattttt agaggaaatt agatttgaaa
     3541 aatttgaaag taaagattta agtgagctta ttgaagactc atcaaaaata tttgcaaaat
     3601 ctagtgaaga tataatctct ataaaagaat cgttaaaaga aagattaaaa atctcaattc
     3661 catatataga agaatcaaaa attctattgt tacattgtat gagtgaaaga atagatatta
     3721 tgaagttatc aataataaaa ctagagaata gaatcgtgct agattcaaat gaagaaatcg
     3781 ataatgtagt ttttatgtta cttcctaaaa attcaccaag ttatcaaaga caaataatga
     3841 gtgaaataag tggttcatta atagacaata taatttttac taataagata aataaattct
     3901 ctatagaaga gatgacatta gaggtaaaag atatagtttt taatttttat acaaacagat
     3961 taaaagcatt tatagataaa taaaattaat attaaaaagg aaggttgata ctatgagtaa
     4021 agtattgaat gagaataata tatttttagg gttagatagt gtttcaaagg aagaagctat
     4081 aactttagca ggaagaaaat tagttgaaaa tggatatgta aaagaagaat atataccagc
     4141 aatgttagaa agagaaaaag taatgactac atatatggga atgggagttg ctattccaca
     4201 tggtgtaaat gaagcaaaaa aagaaatatt atcttctgga atagttatat tacaattccc
     4261 taatggtata gattttgatg gagaaaaagc atatttatta ataggaatag ctggtgttgg
     4321 agatgaacat ttagaaatac tatctaatat agcagtagtt ttagatgatg acttaacaga
     4381 aagactaaaa aattcaaatg acaaacaagc ttttatggaa gcttttgcca actaatctta
     4441 ggagggattt aaaatgaaaa aggcgattca gtttggagca ggaaatattg gaagaggttt
     4501 tattggaagc ttgttagtta agtcgggtta tcatgtagta tttgctgatg taaatgagga
     4561 gatattaaac tcaataaaca aagataaaaa gtatactata cacataagag atgtagaatg
     4621 tatagatgaa gtaattgata atataagtgc ggtttcatct ataaaagaag aaataataga
     4681 tgagattgta caggctgaaa taataactac agcagttgga cctttagttt tgactaaaat
     4741 agcctctaca attgcaaaag gtataaaagc tagaaaagaa aagggattaa caagtaatct
     4801 aaatataata gcatgtgaaa atgcaatata cgcaagttct tctttaaaag aagaggtact
     4861 taagtattta aataaggaag aagtagaata tttagagatg tatgttggtt ttccaaactg
     4921 ttcagttgat agaatagttc ctcctggtaa aaacgaaaat cctcttgatg taacagttga
     4981 aaatttttat gagtggaatg ttgaaaaaca aggatttaaa ggggaaatac caactatagt
     5041 tggaatgaat ttagctgata accttatggc ttatatagaa cgtaaattgt ttacactgaa
     5101 tacaggtcat gctataacag cttatatagg atatttaaaa ggatataaga ctatagaaga
     5161 gagtataaaa gataaattta tctgtgatat agtcaaaagt gcaatggtag aaagtggaga
     5221 aggattgatt aaaaaataca attttgactc agaagtgcac tataaatata ttgataaaat
     5281 tttaaataga tttaaaaatc catatcttaa tgatgatgta ttaagagtcg gaagggaacc
     5341 attaagaaaa ctaagtgata aagatagatt gattaaacct ttaatgacag caaaatcata
     5401 tggattatca gttgacaatt tgattcttgg aataggtgca gcgcttcatt ataataatag
     5461 tgaagatact caaagtgtag aattacaaga gttgataaag tctattgggg taaaaaaagc
     5521 cgttgctaaa atagctaata taagcaatga cgaagaaatt ttaaataata tcgaaaaaag
     5581 ttatatattt atgaaaaatt tataaattta cttgaaaaaa agtagttctg ataaagtaac
     5641 tataatatat tattctacac agtttg
//
LOCUS       DAEPLF010000210         5638 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEPLF010000210 DAEPLF010000000
VERSION     DAEPLF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.211"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(132..431)
                     /locus_tag="KR314_003086"
     CDS             complement(132..431)
                     /locus_tag="KR314_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBH0329449.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QDLINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            638..3679
                     /locus_tag="KR314_003087"
     CDS             638..3679
                     /locus_tag="KR314_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBH0329450.1"
                     /translation="MFKQRISKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGNTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            complement(3840..5114)
                     /locus_tag="KR314_003088"
     CDS             complement(3840..5114)
                     /locus_tag="KR314_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0329451.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(5335..>5638)
                     /locus_tag="KR314_003089"
     CDS             complement(5335..>5638)
                     /locus_tag="KR314_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329452.1"
                     /translation="ELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILI
                     MNKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
BASE COUNT         2063 a          816 c          766 g         1993 t
ORIGIN      
        1 ttactacaaa aactgtcata gaaaaaagcg aaaaaattcg ttctttccta aatttatctt
       61 ataaatattt agatatattc ctacttaata aacagagcta tacatagtat ttaatgtata
      121 acttacctta tttaactcat ataatcaatt attcctgaat ttaaattata taaatgctca
      181 aaaccttctt cagctaacaa attacatgca gtaacacttc tatgaccact tctgcaataa
      241 actactactt tcttatcttt ataatcgtaa atttcatcta tattattgat taaatcttgt
      301 aaagatatat ttatagcatt ttctatttta tattccttaa attcatcttt agttcttaca
      361 tctaataata aaacatcctt ttcatttttc atcatttctt taagttgctt cttatttata
      421 ttcttataca tatgtctaaa attcctccat tttatttata tattacctat accctttatt
      481 ctttatacaa caacaaatat tatttttata tttttctctg ttatataaaa agctatactt
      541 ataaaagctt tttttattga taataatctg ttgattaaaa taattaagaa tgttatgcta
      601 attataaatt attaaaaagg gaaagaggta atgtaatatg tttaagcaaa ggatctctaa
      661 attattatct agtacattag tattgagtat gttatttact gctgctccaa atattacttt
      721 tgctgataat acaaaagata attccgaaaa atatcaaagt tctgatatag aattacatga
      781 ttatagtaaa aacgcggaat cttacacaaa aactaaagct cttgctaaag agaaaatcca
      841 aactttgcta tcaaagtatg gtgctgtaag tgctcaatat gctttgattg ataatggcaa
      901 aattgagatt tctggtaatg gtggagttta cagcaaacag gataacaaaa acttaaataa
      961 ggataatatg tatagtatcg catctatcag taaaatgttt actacaacag ctgttatgaa
     1021 gttggttgat gatggaaaat taaatttaga tactcctgtt gtaaagtata tacctgaatt
     1081 taaaatggct gatgatagat acaaggaaat cactccaaga atgttattaa atcattcatc
     1141 agggttgatg ggaagtagtt ttaaaaacac tattttatta gcagacaatg attcttatgg
     1201 tcatgataat ttcttaaaag aattacaaaa acaaagatta aaagctaaac caggagcttt
     1261 ttccgtttat tgtaatgatg gatttacttt agctgaaatt ttagtagaaa gagtatctgg
     1321 tatgtcattt actaattttc ttgataaata cataaataat ccacttaact tacaaaatac
     1381 aaaaactaca gaaaatagtt ttgatagttc aaaactagca aaagcatatg taccttattg
     1441 ggaagatgca gttccacaag ataaccttaa tgctataggt gcaggtggtt tatattcaag
     1501 tgcagaaaat ctatgtacat ttgcacaaac atttatgaaa aattcaaatg gtattctttc
     1561 tcctgcttca gtaaaagcta tggaaaacaa agaatattta aatggtctat ggcctgaagg
     1621 agaagattct atacttggat atggtttagg gtgggattgt gtcaacactt atccatttaa
     1681 tcagtataat ttaaaagctc ttacaaaagg tggagactct ttattattcc acagtaatct
     1741 tatagttctt ccagatgaaa atatggcagt tgcagttctt tcttctggtg gaagtagtca
     1801 acttaacgaa ataattggac aagaaatctt actatctgca ttaaaagaaa aaggtaaaat
     1861 taaagaaata aaacctgata aaacatttag taagccacaa caagttaaaa tgcctagctc
     1921 tctaaaagaa aatagtggtc tttatgcttc ttcaaatatg ataaaagtag atgttaatga
     1981 taatggtact ttaacagtat cttctcctta tatagaaaat ggacctgaag ataaatacgt
     2041 atacataggt caagatagat ttgtatctga aaagggtaac agttgtttaa aatttgtaaa
     2101 agaaaaaaat aatatcacat atttaaatat gagctcttat gatgatgtcc ctggattagg
     2161 tcagacagca agtttatact atgtagctca aaaagtagat gacaacaata tatcaaatag
     2221 tgtcaaagaa gtgtggaaaa aaagaagtgg caaaggttac tatctagtgg atgaaaaata
     2281 tacttctcaa agctatatgt ttggctctgt aaaagcaagt tttagtcttt ctgatgaaac
     2341 tccaggttat atagttaaca ctaaaataat ggatgagaat aactcaaatg cttttataga
     2401 aatacctggt gtaataggtc gtgatttatc cgacattaaa cttcataaag aaaatggtac
     2461 agagtattta tcttttggaa cactaacgta tgttagtgaa gattctataa ctaatttgcc
     2521 agcagaaaaa tcttttactt gtgaactaga atccaatggt tatgcaaaat ggtataagat
     2581 tggtgacgat atcgcaaata aaaaaataga agttaatctt cctcaaaatt cagcttttgc
     2641 tgtttatgat gataaaggtg ttcctgtaaa ctattcttta gttacaaaga acaatagagt
     2701 tagattgcca aaaggaggcg taattgtttt cttaggaagt ccaaatgcta gatttgaggt
     2761 tacatatcaa gatgaagtaa atgcaagtgc acttacagga actgatagat atgaaacttc
     2821 tattaagata agtcaagcag gttgggaaaa tgctgaaaat gcagttttaa taaacgattc
     2881 agcaattgca gatgctttgg ccgcaacacc atttgcatac aagaaaaacg ctcctatatt
     2941 attgactgga agctctcaaa taaatgaaaa aactttggct gaattaaaga gattaaaagt
     3001 taaaaatgta tatgtagttg gtggagaggc ttctataaat gaaaaatctt tagatactat
     3061 aaaatcaaac aatatctctg tatctagaat ttcaggaagt gatagatatc aaacttctat
     3121 gaacattgct aaagaattga acaatattag caatatatca aagatttcag tagttaatgg
     3181 cgaaaaagga ttagccgatg cagtaagcat tggagctgta tcagctcaaa atgatatgcc
     3241 tataatactt actaatgaaa atagtaatat aactgaaata aataacttat ttaaaaacaa
     3301 aaagattgat aaatcttatg taatcggtgg agaatataca gtttctaaaa atatagaaag
     3361 taaacttcaa aatccacaga gaatatcagg taacactaga aatgaaacta atgcaaaagt
     3421 aataaaagaa ttttacaagg attctaagat agacaattta tatgttgcta aaaatggaat
     3481 gaataaacaa gatgatttaa tagacggatt atctgttgga gttttggctg gaaaaactaa
     3541 atctcctgta atgctagtag gaaactcact agattataat caaaaagaac tatttaaaac
     3601 tatgagattt aaatctgtaa cacaaattgg tggaaatgga aatgaaaact catttaagca
     3661 aataaaagaa atagcttaac ataactaaaa aaggctgtat tcaagtaata ttaattgaat
     3721 atagcctttt tttatttaag ttataagttt tatatgtgta aaaattaaat ttaaatatta
     3781 aatgataagt ttattaagat atagataagt ctaaaacttt gcctttttta agttagtttc
     3841 tatttatcat tcatacttaa caatttatct aaatatactg actttaattg tttactggct
     3901 aaaatctttt tagctggttc aagctttaat atagctgaca gtatagctcc catagctcta
     3961 tgactagcca gagcattatt atcaaaaatt aaattttgta actgcccttt ttcaattgcc
     4021 atgagaactg ctctatgtac tgaatttgct ggagttatta ttttttcatc tctctttaat
     4081 aacataatgc tatttttatg gcaatttcta acacaaactc cacaacctaa acatctatcc
     4141 tcatcaattt ttatatattc tttaccatct tccttaacct tgcttatcgc atctattgga
     4201 catgcagtta tacatttacc acactttaca caattttcat ggtttatatt tggtataaaa
     4261 cttgttgtct gtactggatg aagatttcca aatcttttag ctgctaccat tgcttcacag
     4321 caacatccac agcaattaca tataaacgta acccctttcc taacattttc tccacattga
     4381 actaagttat gttcatatgc ttgatgaagt agttctttac attctatttt atccacacgt
     4441 cttgcaaatt tattatttat taaagaattt gctacattgt caaacgtcat acatatatcc
     4501 atgggagcat cacaagcttt tccaacatgt tgcattctat gtctacagta acacatactt
     4561 atacctatat gagttgattc atcaattata tgagtagccc tttcataatc taaaatacta
     4621 acctcattat cattagttaa aacttcctct tgaacgtata ctcttccaag cttcgtttct
     4681 gttgaataaa ataaatcttt gataaaatct tcttctacat tcatatattg atagtaaagt
     4741 tctgccaaaa gtttttggtc tatatcgtgt ctcgttctca tcatggcaaa ctcaaaaaaa
     4801 ccagccattg gaggaggcat tatatatttc ttccctttat tatcttctat atctagtatc
     4861 agagccttac tagcaagttt atccaatact ctatatgctt cactttcact tacactccaa
     4921 attttagctg ctgtttttac tctgaaaggc tttattggaa gctgagcaac taacttcgcc
     4981 tcttgttctg tatatagtac atttagtatt ttgtataatg tatcagatgg tggagcacct
     5041 tgtggaaact tgtttattct ttcctctaaa cttttataca tattcttcgc agttatatga
     5101 gaaatagtaa tcaccttctt tttcttttgt tttcaaataa aatcaaatat tctttcttat
     5161 tatatattct cttactttat ataataccaa ttctttctaa atagttaaat acctctagta
     5221 atttattatt agatttctta tataaataaa aaatcattta agtgatataa taatatttta
     5281 taccacttaa atgattttaa taatatttta tatagttaag aattattttt atatctatat
     5341 actttctctt cttatattat ctattatatt ttctttattt aatttactca ttggtatata
     5401 ccctgctata aaagttatta taaaaacact tactatacag ataactatgg ctgtccacgg
     5461 tataatcgga gaaaattcaa ttacacctga aataagttta ttcataatta gaattatacc
     5521 aatactaatt ggtattccaa acaacaatga cagtatacca tatataaaac tttctaaata
     5581 tatcattttc ctaaatcctt taggagtaac ccctatagat tgtattactg caagttct
//
LOCUS       DAEPLF010000211         5617 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEPLF010000211 DAEPLF010000000
VERSION     DAEPLF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.212"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(68..1282)
                     /locus_tag="KR314_003090"
     CDS             complement(68..1282)
                     /locus_tag="KR314_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBH0329453.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            1697..2413
                     /locus_tag="KR314_003091"
     CDS             1697..2413
                     /locus_tag="KR314_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBH0329454.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            2548..3174
                     /locus_tag="KR314_003092"
     CDS             2548..3174
                     /locus_tag="KR314_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0329455.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(3391..4935)
                     /locus_tag="KR314_003093"
     CDS             complement(3391..4935)
                     /locus_tag="KR314_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0329456.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AVFELATVNKSYTELKEILFSNSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(4961..5602)
                     /locus_tag="KR314_003094"
     CDS             complement(4961..5602)
                     /locus_tag="KR314_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBH0329457.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CTNLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTVDGANTFERDWSKVNK"
BASE COUNT         2026 a          751 c          799 g         2041 t
ORIGIN      
        1 ttatttaaca gccacttagg tatttaagag cgatagaatt gagtgtatat caaattataa
       61 taatttccta gtaaataatt gaaaaataag gtagtccatt tttatatttg atatctggtt
      121 taccttgaat aagtggatat agataattta gggcttcatc agttatataa ttacattctt
      181 catttatcca ctctttagga aaatattttg tcttgttagc tattttagaa atatcagtat
      241 gactaacttc atacttataa ggattattgc atgttctatt aatgatagcc ataactcctg
      301 tatatccttt tttagatagt tctatcccat atgcaccaac ttcagctgct tgttttaaat
      361 cagtatctga agctaaatgc atagcacatc tttgtattac accaagttct aaggttttta
      421 ctcttgttgt aattgatgat gatactataa gattctttag atagttgctt actccaccta
      481 attgggaatg accaaattta tcatgagcag aagttttttg ttcgcatata aagttcccgt
      541 atttatccct tataccttct gatgcaacta tataagcctt atttttttct ttaaatattt
      601 ttctaacatc ttctataaac ttatcttcat agaaatcaat ttctggaagg tatattaaat
      661 caacaagtgg agtatcatta agtctagcta gtgaagaaga agctgctaac catccagtat
      721 ctctacccat ggtttctact ataaatatac cattattttt gtatatagaa aaatctaagt
      781 atgtttccat tattagtgta gaaataagct ttgcagcaga accaaatcca ggacagttgt
      841 cagttcctaa taaatcatta tctattgttt taggaatccc tataaatttt attttacaag
      901 tagtgttttt gcagtattga gaaaatttca tcactgtgtc catagaatca tttccgccta
      961 tataaaataa tgtatctatt tcatattcat ctagtatatt tataactcta tcataatcat
     1021 aagaatttat tttataatca gccagtttgt atctacaaga accaagacca gacgaaggtg
     1081 tttgctttaa atttaataca ttatctttgt ttaaggagtc tgaatctatt atatttttgt
     1141 ttaatatacc ttcaatccca ttgataccaa aatatacttt atcaaagtat ttagtttcta
     1201 aatttttttc ataaatacca actgcagaag cattgataac agctgtaggg cctccagatt
     1261 gggcaataat acagtttctc attttacatc tccctttcat taatattgtt atgaatggta
     1321 ctttttatcg agtaaattta tatttatagt ctgattatat attaaatgaa ctagctatat
     1381 caattgagtt agtatagaca ataaatgcca taagaatgtg gtaaaaaact aactttttat
     1441 atacaattaa tgtctataat ttaccacaat ataaaggtac ttttaaggag tagtcaaagt
     1501 gttgtgaaaa aaaatatatc agtgtaatat taatttacaa agcaaaataa gttcacagtt
     1561 aacaaaagca gaataagttc ataaaagaaa tatatacagc aataaaagta atgctaaata
     1621 aaagtaatac tggattaaaa aatatcaaaa actaatattt aatactaatt aaagtaaaat
     1681 atggaggtat acaaatatga aacacataaa aatagcagca ggattagcac atgtaaatta
     1741 cggtcatata tcagccgtag ttaaagaagc aatggatgca ggtgtagact acgtacattc
     1801 agatgcagca gatatgcatg acttaaaaaa tatgcaactt atgggtggtc atcaaataat
     1861 agaagcaata agaccagtga cagataaacc aatagaatgc catatataca caagagattg
     1921 tgatagatta tttatagaaa aaatagcagc agcaggatgt aacttgttga taataccagc
     1981 agaacatttt ttaggcgctc ctcttgcata cataataaat tactgtagag aatttggtat
     2041 gaaagtagga ttaacaattg gatgttacac accactatgt tttgttgatg aagctatata
     2101 tgatatagat agattgcaaa tagtcgttca tggtgttgat gaaactgatg gaaaagataa
     2161 ctggggatgg agaaagagtg ctgttgattt agtaaaaaga gctagaaaaa tgatagacga
     2221 aaagaatcct aagtgtgaat tagcaataga tggtggacta agagcagaca atatggagcc
     2281 attaatagaa tgtaatccag acgttgtaat attatcttca gcaatattca aggataaaga
     2341 tggtataaca gcaggtgtta agaattgtag aaaagctata gatgaagcag ctacaaagtt
     2401 tggtttagaa taagataata aactatgata aaagagatgc tttcttaatg ttttaaggaa
     2461 gcattaattt tatgaaaaac tacaggaagc tagactaatt taattgattt tttatactga
     2521 aacaatataa tcataggagg ttattaaatg aaaaaagaaa tgataaaaga ttatttactg
     2581 ataacaatat cagcattttt aatggcatta gcagttaatt ttttctttgc agaacataca
     2641 ttagctccag gagggataac aggattatca gtcgtactta gcacattctt atcaatgcct
     2701 gttgagaata tatcattagg aatatctatg ccacttttag taatgggatt attattttta
     2761 gggaaaggtt ttggaataaa aacattatat ataactctta tgggtccatt attcttaaaa
     2821 gtaataccac aaactcacat aactgacaat ctattagtag caggagtgat aggtgggtta
     2881 ttggtaggaa ctgctatagg gatagcaata ataaggggct gtagtactgg tggaactgat
     2941 ttagttgcaa tgcttattaa taaggtaatt aagtttctga agttgccagt tatattattt
     3001 atgctagatg gatttataat agtagcatct ggaataataa gtaagaattt tatgatatct
     3061 atatattcat tgatatcctt attactcata ataaaaacaa taggatttgt aactacaaag
     3121 tttggtaaga atactgaaga aattaataat ataaataatg cttgcgctca ataaaaataa
     3181 taaaatttac tttaaatata aaagtttaaa aaatataaaa gtttaaaaaa tataaaagct
     3241 taaataaaaa gattaaatat aaaaaatgaa ggtgtctcaa aatgaatttt ttagttcatg
     3301 aggcactttt tctatgaaat caaatatatt tttatcattt ggacaatgaa aaataggttt
     3361 atctctatgt tgagacaagc ctatttttgt ttatttataa gataatattt tatttttttc
     3421 agaatatagt ttttttataa ttgtactaag tctggaaata tcattcttac ttaaaagagc
     3481 atttatattt atcagttcag cattatgttc aaagtctaca ggagcagtgg ttagtattaa
     3541 gtccacatca tcaaagttcg taaatgaaat agaagataga ggtatagatt taactatatt
     3601 aatctgatta aattcaaaac ttaacttttg gactaataaa ttagatacac catcactaca
     3661 gtgacatatt aaaatagtat ttaggggaga tttgcttcta tctattgctg atattaaata
     3721 taaagcaaga tatcctattt cgtcatcatt gacttcataa ttcatcaact gtttgataac
     3781 agtattagac tctttagcag ccttataagt ataaggaaat gttctcttta tatcatttaa
     3841 tatgggattt gtaatagtaa atccatagtt tattctgtgt agaactgatt taaggtgatt
     3901 agcaagagac tttattaata ttaaatcatc ctttaagttt aaatttagta tacttcccca
     3961 atgaacagct attgcttcag atagattata ctctgtaatt tcttcatttt taagaggttc
     4021 aagagtagta gttttcttta ttgatttaag taatccttct atatgaagaa gtatataaca
     4081 gacctcttca tcaataaatt ctacgtcaaa agcttcactt aggttagcac ataaaatttt
     4141 tgatttttca aagtattcat gttctaataa ttctctaaaa aaaccttcag ataagttaat
     4201 atatctttta attgaaattc tcttaataca tatagctata tgtattaaga agtttatata
     4261 atactcatca ctaaggttac ttttttctac ttgtaaggtg tgtagagtga tttgagataa
     4321 tttctcaaaa tcatagttaa atagctcctt aaatattttt gttgatgggg tatctttgca
     4381 ttcattagag ttcgagaata atatttcttt aagttcagta taacttttat taacagtagc
     4441 aagttcaaaa actgcttttc gaatatcttt ctcttttccc tctaaatata ctccattatt
     4501 ggtttttcta actagagtca ctttaaagtt ttgcaaaaaa ttttgaataa ctaccaagtc
     4561 tttatgaaca gtagctctac taacatgtag ttcattgcaa atatcacgaa tcctaaactt
     4621 agaattttta ataaataatt tacttagaat ataccttttt ctatcttcag gagagtatgc
     4681 atatatacaa tttgatttat cattgatatc tctaataact ttaagtttta tatcttcatt
     4741 acctagaata ataacccctg aaccagtttt tttatctagg gataaattaa actctaaaag
     4801 ccattcatct aaaataacaa ggtcattacg aatagtttta ttactaactt ttaattcttc
     4861 ggcaatagta tctacagtaa tatagtctga gctgttgagt aataattcag caatatctaa
     4921 taatcttttg ttcaatgcaa accctcctaa atcttagtta ctatttatta accttggacc
     4981 aatctctttc aaaagtattg gcaccatcta ctgtaagctg gttaaaagaa gcttttttaa
     5041 ataaatcata tgttacagct gcataatcac atccacaaag agatgcttga gttaagtgga
     5101 atacattttt aatagaagct gcaacaactt tagtattata tccatttatt gatataaggt
     5161 tacatatttc tgtaaggttt tttataccat cattcccatt ttcatcattt ctacctataa
     5221 aagggaatag gtaatcagca cctgcattta tagctaatga tgcttgattt acattgaaaa
     5281 tcaaggtaca tgcagttttt acacctttac tatttaatat gtttataagt tctaagccaa
     5341 cttcagagaa atttattttt attatagtgt ttttatttat atttaatatc ttatcacatg
     5401 aattaattaa ttcgtataaa ttatttccaa tgacttcagc agttaatatt tctggattta
     5461 aattagtaca tgtctcaata aattcatgga agtttacctt ctctttaaga tacatagatg
     5521 gattagctgt tattccctta aatccatact ttaaacactt ttgaatttca tcaagattag
     5581 cactgtctat caaatatttc ataatttaat tctcctt
//
LOCUS       DAEPLF010000212         5577 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEPLF010000212 DAEPLF010000000
VERSION     DAEPLF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.213"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            237..998
                     /locus_tag="KR314_003095"
     CDS             237..998
                     /locus_tag="KR314_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBH0329458.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNKKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            1063..1845
                     /locus_tag="KR314_003096"
                     /pseudo
     CDS             1063..1845
                     /locus_tag="KR314_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            2112..2531
                     /locus_tag="KR314_003097"
     CDS             2112..2531
                     /locus_tag="KR314_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBH0329459.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERIFNLKQEKRKAKHKGH"
     gene            complement(2634..2900)
                     /locus_tag="KR314_003098"
     CDS             complement(2634..2900)
                     /locus_tag="KR314_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBH0329460.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSMIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            3127..3828
                     /locus_tag="KR314_003099"
     CDS             3127..3828
                     /locus_tag="KR314_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329461.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            3910..4518
                     /locus_tag="KR314_003100"
     CDS             3910..4518
                     /locus_tag="KR314_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH0329462.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            4842..5306
                     /locus_tag="KR314_003101"
     CDS             4842..5306
                     /locus_tag="KR314_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HBH0329463.1"
                     /translation="MKKLVIILISIISVVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFAISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
BASE COUNT         2284 a          520 c          963 g         1810 t
ORIGIN      
        1 aatattatta aaatgtatat ttaaaatgat aaaaaaagaa ttagacaagt atttagtaaa
       61 ttgattatgt aaaattctat caagcaggaa ccctatgact ttagtcatgg gaggttcagt
      121 aggtaacttg ttgaattaag tttatattat ttaaataaac tacttaattt agataatata
      181 aaaagtcaga attttaagaa tagagctata taatcttagc tattaggaga agtactatga
      241 taggaatata ttttagtgga acaggaaatt caagatactg tgtagaaaaa tttttacaag
      301 aatatgatat aacagcaaat tcttattcta ttgaagataa tgaactttta cagcacatca
      361 ataatcatga gaatattatt tttagttatc ctgtgcaata tagtaatgtg ccaaaaatat
      421 tgaaagattt tataataaat cattcaactt tgtggaatgg taaacgtgtt tttattattg
      481 caactatggc tctatttagt ggagatggct ctggtgtgtt agctagaata ttaaaaaaat
      541 acggtgctat aactgttggt ggattacatg tgaagatgcc agatagtata ggagatgaaa
      601 aggtattaaa acattcttta gagtataata aaaagcttgt aataaaagca gaaaagaaag
      661 taactaaagc tgctaaaaaa ctgaaacatg ggtcaccacc aaaagaaggg ttaggttttt
      721 tgagtcatat tgtagggtta tttggacaac gattgtattt tatcaataaa acaaatatgt
      781 atacagataa actaaaaatt aatactgagg aatgtattgt atgtggtaaa tgtgttaatt
      841 tatgtccaat gaagaatcta gtaattaaaa attgtatgat tgttgcaaat gggcaatgta
      901 caatgtgtta tcgttgtgta aataattgtg caaaaaaagc aattacattg ttaggtaaaa
      961 aagttgttga gcagagcaat attaaaaaat atatgtaaga aatttaaagt tttattgaat
     1021 gtaaattgat gtatatttta tgttggaaag agaggtcaaa taatggaata ctacatagtt
     1081 gattcatttg caactaaact atttaaagga aatcctgctg gagtttgtgt tcttgacaga
     1141 ataatacctt tagagttaat gcagaaaata gcagaagaaa acaatttacc agaaacagca
     1201 tttgtagtta aaggtaaagg aaattatgaa ttacgatggt ttactcctaa agcagaaatt
     1261 gatttatgtg gtcatgcaac tttagcagca gcttatgtaa tatcaaattt tattgatgtt
     1321 aatgtaaaaa ggatagattt ctttactcaa agtggaaagc ttgaagttac acgaaatgga
     1381 aatctatatg aaatgatatt tccagaaata atgcctattg aaatagagtt atcaccataa
     1441 caagctaatt tgataggctg tgtaccaagt gctgtctatt cttcaagaga tttaatctta
     1501 ttgctaaaca gtgaacaaga agttataaat tataaaccta attacgcaca attgcgtaaa
     1561 ttgacagact ggttaggtat aataatcaca ccacaaggaa gtaatacaga ttttgtatca
     1621 agatattttt gtccagaatt agattcagaa gaccctgtta ctggttcatc acattgtaat
     1681 tttattccat actggtctga aaagttggga aaacacaaaa tggtagctgc tcaactttct
     1741 aatcgtggtg gtataattca gtgtgaagtg ttgaaggata atactgtaaa aatttctggt
     1801 gaagcagtct tgtttatgca gggaacaata aagattgata tttaaataag tataataact
     1861 aatttattat gaaaaataat taattttctt agtgttaaca ataataaagt agagttaaaa
     1921 agcttgattt cacttaaatc aatatgatag atgttgatac taagattgac aagcatgatt
     1981 aaaaaagata atatttaatc tataaagata aaaacacagt ctggttggta gtccagacgc
     2041 agcaatagct gtcagtaacc tgcctccttg gttgtccgtt ctttagaaag taatttaagg
     2101 aggaactagc gatggacaaa attagtggaa aattaacagt cttatttgaa gaaccatttt
     2161 gggtaggtat atttgaaaga caagatggta aaaaatatga agcatgtagg gtagtttttg
     2221 gagcagaacc taaagaagta gaagtgtatg aatttatact tgaaagattt ttctcattag
     2281 attttggtag cattaaatta gaaaaaaatg tgaccaaaga caacattgga tacaaaagaa
     2341 tgcagagaaa agtaaaaaag gaacaagaaa aagaaactat agggactaag gctcaaaatg
     2401 ctcttaagct tcaatatgaa gaaagaaaac aggatagaaa aaacctagct aaaaatagaa
     2461 aagaagaaga aaaagaaaga atatttaatt tgaaacaaga aaagagaaaa gctaaacata
     2521 agggtcatta attttaatta aatatctttt taataattgc tagaaaaagg tgtataaagt
     2581 ttttaatttt atacacctta atttattgaa aagaataagg ttttatattt caattacttt
     2641 aaaatataaa aatatactaa taaaaattgt aaaaatagta taaatggtat accaaatgta
     2701 aatttaatgt gtttagtttt atgtctaaaa gaatacattc caatcataga acctatacta
     2761 cctccaatta ctgctataga cataagagta gcttctttta ttctccattc gtttctaatt
     2821 gctcttttct tatctatgta cattgaaaag aaagcaatag aattaatcac tataaaatat
     2881 attagaataa aatttttcac agtcttgcct ccataaaaat atattataca ttataaaaca
     2941 agatatttgt tttggaaagt taaatttgta attagtacat ataaaatatc tgataagaat
     3001 aaatataata ttaattggaa aatttaaaaa aattaaacaa ttagcaaata gggggtatat
     3061 ttataagaca caaagtgata aaatctattt agaaaataat gtaaaataac aggaggataa
     3121 agcaaaatga aatttatagt agaaaaagaa gtttttgata aattagaaaa tgtatgcttt
     3181 ggtgtagtag ttgcaaaagg aatagataat actaaagaaa tagaaagaat aagcaacctt
     3241 ttagatataa gtatagatag agtagaagat tacttcaaag ataaaaaagt aaaggaatca
     3301 gaagaaatta taccatatag agaggcattt agaagtctag gcatgaaccc aaataaattt
     3361 atgagttcga tagaagcaat gacaacaagg gttgctaaga ataagaaact tcctcatata
     3421 aatcctattg tagatttagg aaactctatt tctttaaaat atttacttcc aatgggtgct
     3481 catgatatgg attttagaaa tgatgatgta tatgttagat tttctaaaaa aggggataaa
     3541 tttgtaccat ttggagaaac tgatgttgaa ttaatggaag aaggagaatt aatttattca
     3601 gttggagaca tggtaaaaac tagaagatgg atatggagac aaggagagga aggaaaaatt
     3661 actaatagct caaaaaatat attttttcct atagatggtt ttactgatgc taatttagat
     3721 aaggtaatga gtgcaagaga agaattagca aaattgttaa aggaaatatt taattgtgaa
     3781 atcaaagtag gttttgtaga taaagataat cctgaaatgg aaatataata agttgttgaa
     3841 tacttgttaa gatattatat ttacttaatg atgttaaaaa ggtattaaaa aagaaaggtc
     3901 gtagtaaatt tgaaaaaaac tataataaag aatgtaaatc caatgattga aaataagttt
     3961 ataggacttt ttgaaataga gtataaaaac aaattagatg aagataaggt ttggatggta
     4021 gcatcaagaa aaagtagtga gcaattaaaa aatatttatt tgaagaatga agaagatagt
     4081 gtagatgcag ttgtaatagt tgggcttcat aaaagtagta aaaaattaat tttaataaga
     4141 caatttagag tacctattaa tggatatata tacgagttac cagcaggtct tgttgatgaa
     4201 ggagagtcta tagatatttc tgttgaaaga gaacttagag aagaaacagg acttactctt
     4261 ttagaaatta aaaaaaataa aagtattgat aaagtttatt tatctcctgg aatgagtgat
     4321 gaatctgttg cctttgttta ttgtatatgt gatggtgata taacagatga atttttagaa
     4381 ccagatgaag agattgaagc gcttttaatt tcgcaagaag atgcaaaaga gattttacaa
     4441 agtaatcata agatagacac aaaagcattt ttgattttac aaatgtttgt gagtttagga
     4501 gctaaattat ttgaatagat atatagaaaa gaggttatat agaagagtaa aataataaag
     4561 tttattttac ttgtataaat tataatctct ttttttatac aataaagtat aattttgtgt
     4621 atttttaaat aagttacttg actcttaaat tttcataata tgttataaac caatttaaga
     4681 tactacaata attaaaattc caaaattatt tagcatattt gcaattaaaa tgagaaaagg
     4741 taaaggctga atgattgaaa aatttgatgt aaaatagtag attagtttaa tattatagtt
     4801 tgtagggtta aaaattagaa attgtaaagg agataaattc tatgaaaaaa ttagttatta
     4861 ttttaattag tatcattagt gtggttagct tgacagcttg taaaaaaagc aatgaaagta
     4921 aatataataa tttaaacaac aaagaatcag gacaaacaac cattgaaatg gacttagata
     4981 aaaattatga tacttcagac ccatttgtta atgcacgttt gttttgtgtg tctaatgata
     5041 tagatgtttt agacacagag atttctttta aaatggatgg agatagtggt attgtggaaa
     5101 taaaggacaa taaaacagat gaaactttat ggagtaatac atggcatgga agcgttgata
     5161 atgatacatt cgcaatttct ctttctaata tacaaaaaga gaaagagtat gctatatgct
     5221 ttaccggaat taaaataaat catgcagttg taaaggttac ttttgaaagc aatttagtta
     5281 aggagaaaga aaaaccctca aaataaattc cagcttgtct ataacttgat attcagtaca
     5341 taaaagcagt tgatatttat gattgactgc tttttcctat caaaaaggat aggttgtgca
     5401 tttttacttg aaatagttta tgcacatcaa aagttggtga gcatgatatt ggaaatggta
     5461 agcatgaaat cttgataaca caaggaaata aatttaagtt ttcaattaag tatatttcca
     5521 taatttagtt aacaataagc taattattat ttatattttg atatataaat aataatt
//
LOCUS       DAEPLF010000213         5569 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEPLF010000213 DAEPLF010000000
VERSION     DAEPLF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.214"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(38..1018)
                     /locus_tag="KR314_003102"
     CDS             complement(38..1018)
                     /locus_tag="KR314_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBH0329464.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            complement(1075..2277)
                     /locus_tag="KR314_003103"
     CDS             complement(1075..2277)
                     /locus_tag="KR314_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBH0329465.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            2472..3026
                     /locus_tag="KR314_003104"
     CDS             2472..3026
                     /locus_tag="KR314_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBH0329466.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            complement(3141..3932)
                     /locus_tag="KR314_003105"
     CDS             complement(3141..3932)
                     /locus_tag="KR314_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBH0329467.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKPDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDFSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFKGMSGTEEILSNILDENCLLNI"
     gene            complement(3960..5048)
                     /locus_tag="KR314_003106"
     CDS             complement(3960..5048)
                     /locus_tag="KR314_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBH0329468.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            complement(5102..>5569)
                     /locus_tag="KR314_003107"
     CDS             complement(5102..>5569)
                     /locus_tag="KR314_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRSP domain-containing protein"
                     /protein_id="HBH0329469.1"
                     /translation="NKKYVKYTGRFGINRREQFRLKEIVKRESKKITMASKESLKNEK
                     EKILFLGTEEFMYIPMLFAKEKENQYDIYYHSTTRSPIINIDKKHYPIKSKFMLKSFY
                     NIDIQNYIYNIDKHNFSKCYLFVELNKSENSYLEFIDIIKKTSIKELTIVCCS"
BASE COUNT         1886 a          931 c          581 g         2171 t
ORIGIN      
        1 aaatatacta ttttataaat ttaagttctt tattatatta ttttagtata tctttaaaga
       61 gcctataaaa attaccacta gagattaact ctatatcttt ctttttataa cctctatttt
      121 caagctctac tactatgttc tgtgctgtat gtgtgctttc aagaccatct acacttggca
      181 cactagcatt tccagtaaaa gaacctatag tctctttccc taagtaatca ctaaaatcaa
      241 aaccaaaaga tacatgttct atacctataa gatttaccat atagtctata tggtcaacca
      301 aatgtattaa atcttgcttc ttaggattac tattagtaaa tcctctaaaa ctatttattc
      361 caactaatcc acctgtattt gaaatttctt ttaattgatt atctgtaaga tttcttttag
      421 catcacataa actcctagca ttagaatgag aagctataac tggcttactt gcaacagaca
      481 ttaaatccca aaatgatttt tcatttaaat gtgacacatc tactatcatg cccaattttt
      541 ccattttatt gactgctttt ttacctattt cagttaaacc acgattttca tcccctaaac
      601 atcctgtagc aagttcattc tgttcattcc aagttaatga tgcatgtctt gcaccaactt
      661 ctttataaaa ataatctagt aaatctatat ttcttccaat atggcttaat ccttcaagac
      721 caattaaaat atttattttg ttttcactta tacctttatc tatatctgaa tattttttta
      781 ctatgtttac aacatctttc atatcttcaa tttcatcttt tatagagctt ataatttgtt
      841 taactctttt ttcaggctct ttatcatatg gtgggtctat ccatattaca aaaatagctc
      901 cttctatatt tgctctttta aaattatcaa gatggattct tctaaataca tctttttccc
      961 cgttaagcct tcttattgtt acatctgccc aagtatctga atgtccatcg aaaatcataa
     1021 caacctccaa ttaaataaaa atctttctaa aaaacaactt cttttcacgt aaaattatat
     1081 caaaatattt cctactaaaa gaccaaaata attaccaact atgtatccaa atgttccaac
     1141 tataagtatt ggccctacta gcttagacca tccttttgat attgccatag ctgctgctgt
     1201 tgtaggacca ccaatatttg cattagatgc aagaatagct tcttctaaac taaacttaaa
     1261 tatttttgca actataaatg taactagcat gtttattagt accattattc cgcagtataa
     1321 aagtagtaat ggtgattttt gaattattaa tgttattgaa gcaggaacac ctactacaac
     1381 aaagaatata tatattaaaa atgtacccaa ttcctgtgct ccttttatat caccaaagaa
     1441 attaggcata aatgtggcaa acaacatagt caaagtagtt attattagat attgatttcc
     1501 aaataaagta tttaacattg ctaaaaaagg atttgataat ggtattatag ctttaaacca
     1561 agttgatagt tctacagata ctgcgacaat tataaaggcg ctaccaatgg ctattgctat
     1621 atcttttaaa gatatttctt ttcttcccca ataatttgct gctactgttt ctccttcagt
     1681 tatacctata ctttccattt catctacatg tggatgttta aaatgttttc taaaaaatcc
     1741 aattgatggt atagctatta agacaaagaa atataatgcc attaaaagat tatctgctac
     1801 aactgttgct gatactaatt ctcctggcgc atcaaacgcc cctgacatag cagcaaagtt
     1861 tacatttcca cctatataag aacctgtcat catagctgca acaatattta aatctggtac
     1921 tacatttttt aatgctaaaa atccaatcat agctccaagt acagttccta cagaacctac
     1981 aagaaatata atgagcaatc ttccactttc tttccatatc tttttaatat tacattgata
     2041 taacaataaa gctatggcta aaggaacaac atatccccat acttggtcat atacaacaga
     2101 ctctactggt ataatattta gatttgataa taacattgct cctacaagag ctattattgc
     2161 tcccgacact tttgatgccc aattgtactt ttgttccaag taaatactaa atgctgccca
     2221 tccagttaca actgcccata atacccaaat attatcagga cttacaaaac tcttcatctt
     2281 aattacctcc atttcattaa attaaaatat cattatcaat attcaatata ttgaatatta
     2341 atactattat aatgcatctt ttcagaaaat tcaatataac ataaaaaata catgcattat
     2401 aaaacttttt tttattttat taatctttat aaattatatg ttataatcat gtctgagggg
     2461 gtttgtatct aatgaatata gatgtcggaa gcaaaattag atctcttaga aaatctaaaa
     2521 atataagtat ctctacttta gcaaaaaatt cagatttaag tacaggctta ataagtcaaa
     2581 ttgagcgtaa tatggttgtt ccatctattg tagcaatgtg gaaaatatca aaagctcttg
     2641 atataaatat aggttatttt tttgaagaga ttggaagaga ggactctgat atagtagtaa
     2701 agaaaaataa tcgaaaaaaa atagtaacta atgattctac aaaattttat gaacttcttg
     2761 ttcctaattt atctgggaaa aaaatagaat ttatacttgt tacgctagat ggtcatacac
     2821 aaaaaaacag tgaatttgtg acacacgaag gagaagaatg tggctatata ataaaaggta
     2881 aaatgaagat aacacttgga aataaggaat atctattgga ggaaggagat agtttttatt
     2941 ttaacagtac tataccacat gtgtatgaaa attatgatga tgaggtatgt atctcagtct
     3001 gggcaatgac tccaccttca ttttaacata ttgagttctt acttaaatat aaattgataa
     3061 aaatctgaaa taaattagta tgataaactt tactataatt tatttcagat ttttctagtt
     3121 tattatttat atatcagtca ttatatatta agtaggcagt tctcgtctaa aatgttagat
     3181 aatatctctt cagttccact cattcctttg aaagaacttg atataaatga attttctatt
     3241 tcatttttat ttatatcacc atgcttcaat acataggatt tatttgagat attaagcata
     3301 ccatagtcca taatagaatc tccaactgca taaaaatatt ctattcctaa ttctttagtc
     3361 aagtatttta tagcattttc ttttgaaatt tcttttggta taaaatatat ttttctgcca
     3421 ctcacataaa acttccaatt ttttgattcc aatatatttg tatattcttt gattgaatcc
     3481 aaattaaatc ttgagaaatc aacaactata taaaaaaatg tatcttctgc aactttaaaa
     3541 ttagttattc catctacatt caaatagtct ttaaatttat gaatcatact ttcaatgggc
     3601 tcataatcag gttttagttt atctattttt tcactccatg atttcaatat ttcattatct
     3661 ttaagtataa ctcctccatt tgaagttata ctccaaggaa aataaatccc atatttatta
     3721 aattctatac gcttaaattg ctccactgtt ctagtagttg taggtataaa cataccataa
     3781 tattgtattt gctttattaa atttatagta tctaaagata tataagatat ttctttttcc
     3841 ctataaattt ctatattagc atatttacta tcagcattta aaaatttatt ggagtaaatg
     3901 atactcctgt ctaaatctga aaaaacaatc attcaatatt ctcctgtcta atatatttcc
     3961 taaatatctt tcatattttt tattattcca caacatttat aggcttttaa atccatttgt
     4021 tcaacttcaa catttttctc cttagctaat atgaatatat ggtctaaaga tttatcattt
     4081 aagtctttaa caagaatctt atacggaact cttctaagta aaacccttgt cgtttcccct
     4141 acacctggtt tgataaagtt aatatctgtt atgttatact tttcttttat atttaaaaca
     4201 tcaaagtatc catctcttgt tataatgtca ctttcccagt tttcaacttc atcagatata
     4261 ttcttaaatt gatttttaaa acattcttct atagtttcga tatagttcat agattcatct
     4321 ttatctttta aatgtctata aaacttagct ccatgaaagt catttttacc aattaaatcc
     4381 tctcttagta cagttctact tactaaacca gaaacggttg aatttaaaca tgcacttggt
     4441 attaagaaat cttctctaat tccataaagt cctgaatatc cagcagggtc agctaataca
     4501 gcaagtgttg agtcaaaatg ttttttaaat ttaacattaa gttcatcaca tgctttttca
     4561 agttcttttg atatagttcc cttaccagtc catccatcta aaaattgaat ttttgagctc
     4621 ggatgattct ttactatata ttcaattgca ttcatatcta ttcccttatc tcttattata
     4681 gagattgtat agtgaggtaa acttacatta tacttgcttc ttatatatct ttttgccaaa
     4741 ataccaatag gtgttcctgc tctagcaagt gaaactagta ctacatcatt tccatttttt
     4801 ttaattattt tttcacacat aactcctata gaaaatgcaa gcttatcttt attttctctt
     4861 agtttctcat gatataaatt cagataatct tcactaactt catactctat tggtatcatt
     4921 tctgaataat gtctaccatt ttgtattttt aattctcttt ctttatttcc ttcttcttca
     4981 attaaatttg atatatcttt taataaaaaa ataacatcat ctttagaata cgtgctaaaa
     5041 tctctcatat gactcaactc ctatcttaaa atcgtgatat attttatcct gttaaaacac
     5101 tttagctaca acatactata gttaattctt ttatacttgt ttttttaatt atatcaataa
     5161 attctagata agaattttca cttttattca gctctacaaa taaataacac ttagaaaaat
     5221 tatgtttatc tatattgtaa atataatttt gaatatctat attataaaaa ctctttagca
     5281 taaatttact tttaatagga taatgcttct tatcaatatt aataattgga cttcttgttg
     5341 ttgaatgata gtatatatca tattgatttt ctttttcttt tgcaaacagc ataggtatat
     5401 acataaattc ttcagtacct aaaaatagaa tcttttcttt ttcatttttt aaagattctt
     5461 tactggccat agttattttt ttactttctc tcttaacgat ttcttttaat ctaaattgtt
     5521 ctcttctatt aattccaaat cttcctgtat attttacata tttcttatt
//
LOCUS       DAEPLF010000214         5556 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEPLF010000214 DAEPLF010000000
VERSION     DAEPLF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.215"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(51..785)
                     /locus_tag="KR314_003108"
     CDS             complement(51..785)
                     /locus_tag="KR314_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329470.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(754..1767)
                     /locus_tag="KR314_003109"
     CDS             complement(754..1767)
                     /locus_tag="KR314_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0329471.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(1789..2559)
                     /locus_tag="KR314_003110"
     CDS             complement(1789..2559)
                     /locus_tag="KR314_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329472.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(2534..2824)
                     /locus_tag="KR314_003111"
     CDS             complement(2534..2824)
                     /locus_tag="KR314_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBH0329473.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(2955..3054)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      3674..3763
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3816..3992
                     /locus_tag="KR314_003112"
     CDS             3816..3992
                     /locus_tag="KR314_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329474.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(4107..4463)
                     /locus_tag="KR314_003113"
     CDS             complement(4107..4463)
                     /locus_tag="KR314_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329475.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKDVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            5194..>5556
                     /locus_tag="KR314_003114"
     CDS             5194..>5556
                     /locus_tag="KR314_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0329476.1"
                     /translation="MIAVKKAYKFRIYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELNWLKEPDKFSLQNALKDLENAYNKFFKEKAGFPKF
                     KSKKTNRFSYKTNFTNGNI"
BASE COUNT         1953 a          871 c          669 g         2063 t
ORIGIN      
        1 cattttggat tgctaagttt aatgcttatt aaataaaaat atatatattt ttacaataaa
       61 gatattattt cttttttaag tttaatgata ttttcaaggt tatcttcatt taactttcta
      121 ctatctataa caaattcttt ctttactatt gatggttttt ttgaaataac ataaattctg
      181 tttgatagca ttatagcttc gtcaatatca tgagttataa gaagtattgt tgaatttact
      241 tttttcttaa catctaaaag ccatttttgc atggatgttt tagttaatga atcaagtgcc
      301 ccaaaaggct catctaaaag cattatgtca ttagaattta taaaagttct caagaagtta
      361 gctctctgtc tcattcctcc agaaagttct gaaggatatt tatcttcata tccgcttaat
      421 ccaaacacct caaaataagg ttttaccatt gacctagctt tactcttttt ttcttttttt
      481 aaaattagtg gtagtgacac attatctatt atagttttat atggcaagag taaatctttt
      541 tgatacatat aactatattt accatttata ttcactttgc cactatcata atctgtaagt
      601 ttagttataa tattaaaaat agtacttttt ccacatccac ttggccctaa tatagaaaca
      661 aactcacctt cataaacatc aatacttata tcttttaaaa cttcaatatc tttataagtt
      721 tttgatacat tttctataga tacttttact tttttattgt ggtaaaaatt catttgtgta
      781 agcatcactt gccttcatat ccttatctat taatttatat tcttttaaga atgatgtata
      841 gttgttccat acactatctt tcatttctcc ccatcgtggt gcatcatctt tgtacttgct
      901 tgctaaatat tcttgactct ttaaagctag tttttcatct acttcaggtg catgttttac
      961 taaaattttt gctgattctt ctggattttt tattgcatat tcataacctt ttgttgtagc
     1021 ttttaagaat tttttagcaa gctctggatt atctttaagc acagtttcat tagaaattat
     1081 aagtggtgta taataatcca atctctcatc taaatctttt actggtataa agtttaagtc
     1141 atatccaatt aaatcagctt taactgcatc ccaaccttca aatatccatg caaaatctac
     1201 agttttcata gctgcaaaga aatcatcttg accagtatta actatcttta acttattaaa
     1261 atcagctcca tttttcttca taactgcttt aaaaacagct tcttctgatg gagagcccca
     1321 accaccataa gtttttcctt caaaatcttt agctgttgtt atattctttt cttttggtga
     1381 tgcaaaccca gaagtattat gttgtataac agttgctaca gcttttattg gaagtggatc
     1441 ttcactagtc tttgcataag taacttgttc ttgatagctt ataccaaagt cagcttttcc
     1501 tgttgctact aaagtttctg ctccactttc aggtggttgt actatttcaa catccaaacc
     1561 ttcttcttta tagtaaccct tatcaagtgc tacaaataat ccagtatgat ttgtatttgg
     1621 tgtccaatca agcaccatag ttactttttt agaagaagta tctttatcat tgtttttgtc
     1681 tttattgtta tttgaagaac atccagttat tgttgccaat gtaaaaacac ttactaaacc
     1741 taatattcct aactttttaa aattcataat tgcctccctc aaataccttt aatcaattaa
     1801 tctatgtctt ataacaactt tttttacagc ctgtacaatt cctacaaaca ttaaactaaa
     1861 tataactact aatatagtag atgcaaatac tttatcaagt gcatatgcac tttttgacct
     1921 aagcatataa acaccaagtc ctttcgttcc acctagccat tctgcaacag ttgctgccat
     1981 tacagcatac gttgcagata ttttaagtcc agaaaaaaat ttatccattg ccataggaaa
     2041 tttaaggtga atgaatgtat taactttatt tgagtccata gtcttaaata aatttaaata
     2101 atctttatca atattctcaa tgccatctac aaaacttatt aatataggaa aaaaacaagt
     2161 aagcattacc atcaatactt ttggcattgt tccaaatcca aaccatatca taaaaagtgg
     2221 tgctataatt attgtaggta tcatctgtgt aaccaacata ataggataaa gacatttttt
     2281 tattattgat acaaaatcca tcaatattcc aattaccaat gatattataa ttgcgactat
     2341 aaaaccaagt attgcttctc ctatagtaac cacagcatgc atggataaat tttgataatc
     2401 tttaaaaaac acattaatta tatctactgg tgtgggtagt atatactgag gaacttctaa
     2461 aactcctacc atagattgcc aaagaataag tataaacaaa aatgttgcta ttggatatat
     2521 actatctttg actttatcta tgatttctaa gtttttcatc tatagtcaga cctccagctt
     2581 tttcatctac tctagctact gttatgactc tttcacatcc aagattaaaa gggattttat
     2641 gtaccttttt taataattca aaaacttcat caaaatcacc ttcaacagta gtgctcattg
     2701 ctcctatttc acatttaaga ccactttttt caatttcagc aatacaagca tctactactt
     2761 tatacatttc ttcttcacct ttgtaaggtc ttagtggcat tactgctatg tctgcgatta
     2821 ccatttaaat cactccttta aaaataatta atacctacta ggtatatttt gttttttatt
     2881 aaaaaatttt attactaaaa agatattata tttaaataaa agaaaagtct tatccaaaat
     2941 aggataagac ttatttttat cttaactggt ctccctacac tggcattatc caaacaggtt
     3001 caaagggtcg agataaaatc tcctctcagc acattagctc cccgaaatat attatatctt
     3061 ttctacttga ttatactaca cttatcatat tttaacaaat tttttattta tattagatat
     3121 tttatccgtt caaaaatatc taatatacat tgtacattat tacaccaaat aaattatctt
     3181 aaaatggtaa tttatcaaca tcttccaaaa ctagattaac tgaattttct agtttttatt
     3241 ttgctatttc aagcatattt ggatgtatat caattccata caaatattgt tctaaatttc
     3301 tgttttcact aatctaattt gcaatcgtta cagattacag attagaaata gcttttagac
     3361 tctacttatc tttatctaca aaaaatcctt caataaattc agtaataata ttcagtaatc
     3421 taaaaataaa cctctatctg gatttttaag atacaagcct tttaggaagt atcttttgtg
     3481 ataatataaa aattactcgt cattatcatc aaaatcctca gcaaccaaca ttttagataa
     3541 gaatcaaact ctataatata attattttat ccaatttatt tttgagaaat aaaatatttt
     3601 tttaattttt aaatttttta atatgcacct tgaaaaaaga aacgttttcc tatattatgg
     3661 taatagtaga ataaaactaa ataggcaaaa ctagagaaat ttagtgacgc aaagctatag
     3721 ggactaagac ttatgaaatg agttatgtca gccagttgcc aaagagatat tttctttttg
     3781 tttttatgaa tgttttttag ggggatatat gatatatgtt tatggataaa atggatagat
     3841 gtacacatat actaactgca tatatatgca gttcacgcga ttactgcaat tttatagata
     3901 cacaattaaa tgattttata ctagaatatg gagagaatgt ggtagaatct tgtttgcatc
     3961 aagtgatggt attggtaagc agatataatt aaagaaattt tattttctag aaaacccttt
     4021 ttagttttat ctacgtttta taattcatta aagaactaat caaaataagc ttcattatat
     4081 atttttcatg aagtaaacag ttgagtttat ctcatatact gtttacccca attacataat
     4141 tctaataata tagattccaa tgatttacca cgtttagtag acaaatattc aacttttgga
     4201 ggaatagtcg gataagaaat tctcttaata agtccatctc tttcaagata ctttaattct
     4261 tgtgacagta tcttatgact tatatgaaca acatcttttt ttaattgact gtaacgtatt
     4321 ggctcttgtt ttaaaatatg ccataaaata attgccctcc atttacttcc tatcacagtc
     4381 atggtaaaac caattgaaca attaaaatct ttttgtcttt cttcaatatt tggaaattct
     4441 tcaaaattat tatctatatc catctcaatc ctctctttca aaatagtaag tatctaacta
     4501 aaaagtgcat tcttgtttag cgtactaatt cgtaatataa tcttgattaa aaaatagcta
     4561 cgcacactcg tgactttagt catgagttag tagctaaaat agtcaacata tagggaaacc
     4621 tatatgtaga gataggatag aacctatcca atactacttg aattgctaaa aacccctaaa
     4681 gctaaccaaa ctaaaacata aggatgaaat aaacctaagt gtgaaagtta cgaaagtaga
     4741 aaaaattggt tagatggtat aaggttaaac cctaagtact ttaaaatggg taatcagcag
     4801 gtaaactccg aataggagaa acttcaacga ctattcctct tgagggaagt acactacaag
     4861 ttatcggtag tggaagtggg tagaccttaa cggataatgc cgaaggataa gatatagtct
     4921 gtgcttatgt gaaagtgtaa gaagttcata agagaactgc ataggtagta gcgaacttat
     4981 gtgaacgaca gcctcaaaaa tgataaaagt taagacttac ggttcttata tatgttattt
     5041 aaaattaaat ataaaacctt ctaaataatt gttttaaata attacatttg ttaatattat
     5101 gataggtgat taataaattg ttcaccacag tattatataa tatattaaat attaaaatca
     5161 ttaaaaagtg tatcaaaaaa caattaaagt gaggtgatag cagtgaaaaa agcttataag
     5221 tttagaatat atccaaataa aaaacaacaa gagttaatta acaaaacttt tggatgttgt
     5281 agatttgtat ataataaata tcttgctaaa agaatagatg tttataaaaa cgataaagaa
     5341 acttttactt ataaacagtg tagttctgat ttgaccaatt taaaaaaaga attaaattgg
     5401 cttaaagaac ctgataaatt ttcactacaa aacgccttaa aagatttaga aaacgcatat
     5461 aataaattct ttaaagaaaa agcaggattt cctaaattta aatcaaagaa aactaataga
     5521 ttttcatata aaactaactt tacaaatgga aatatc
//
LOCUS       DAEPLF010000215         5548 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEPLF010000215 DAEPLF010000000
VERSION     DAEPLF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.216"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(282..1688)
                     /locus_tag="KR314_003115"
     CDS             complement(282..1688)
                     /locus_tag="KR314_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0329477.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILKDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTIKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGVKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVAGIINAHRGKLDI
                     MSIDSQGLKTILKLPLIK"
     gene            complement(1733..2440)
                     /locus_tag="KR314_003116"
     CDS             complement(1733..2440)
                     /locus_tag="KR314_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0329478.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2602..3525
                     /locus_tag="KR314_003117"
     CDS             2602..3525
                     /locus_tag="KR314_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329479.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     TIMQRLLDLPKKNVNEALKIVRLENQKNKKVNQYSLGMKQRLGIAMAIMKFPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     SIKELHNRGQSQIAIKAKNVVSAQKLLNTKGYMSLIQNGYLTIENLKDSDVAKINGML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3525..4286
                     /locus_tag="KR314_003118"
     CDS             3525..4286
                     /locus_tag="KR314_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329480.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPILMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4298..5035
                     /locus_tag="KR314_003119"
     CDS             4298..5035
                     /locus_tag="KR314_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329481.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1932 a          833 c          718 g         2065 t
ORIGIN      
        1 aaatattaac taaaaaataa aaaaaatgga attatctaaa gaatttaata accttacaat
       61 aattccaaaa tattatttct atgaaaattt tgataccatg agtaactcag ccataagcgc
      121 ataaagcaca agttactctt tactttaata aaacttgaca agaaaagaat ttatccttat
      181 tctactctaa tagagttatt ttgtcaataa agaataaaat tattttcaaa atattttcat
      241 tcttcattct tcattcttat ctgttattat tttataaaat atcacttaat tagaggtaat
      301 tttagtattg tttttagtcc ctgactatca atactcataa tatctaattt acctctatgt
      361 gcattaataa ttccagctac tatcaaaata cctagaccat gattttgacc tttggcatat
      421 gcctcactta gtccattttg gctctttaat aaactattaa ttctttcttg actcatacct
      481 tttccatcat cttcaacaat tatatagcaa aaatctaaat cagacttaga ggtaaccctt
      541 atattacatc cttgttggtt atgagttata ctattttgta ttaaattact aatagccctt
      601 tttaataatt ttttatctgc atctataata atatcttcat ttagtatatc aagttcaaca
      661 taaaagtcat tatccaaatt attatttata aactcagaaa taagctctct caaaaacgca
      721 gaaactctaa tcttatcaca attcaatgct tgcatattat attcaagttt agatactaaa
      781 tttaggtcat ttactaattc tctgatttta accccttgat tgcatataat agacacttgc
      841 tcttgttctc tatttgaaag gctactgctt tcttcaagtt ctccagcata tccaattatc
      901 attgaaagtg gagttcttat atcatgagat attcctgcaa tccagttaga cctagcttcc
      961 tctttcttcc taagtttttc atctttcatt tgcaactctt tagatacagt attaatagat
     1021 tttgaaatat cacttagtat tcctttttct tgtaaaagta ctggttctcc ttttgacata
     1081 agctttatgc cagtaataat tggttttata gatttcacca tttttaaacc tatgaataga
     1141 gctattaaaa aagtaactat tgcattaacc agaaacatac ctattattaa cataggaaat
     1201 tgttctattt ccttaatcgt ataagtaagg ttaaatttag tgtatttgtc ctttggataa
     1261 cctgttacta atattccatt tggatgttcc catacataca caggatagtc ctttaaaaag
     1321 aaccttgaaa actttgctac atctgtcaaa gtataatgac ttggaatttc ttttggtaaa
     1381 tcaaatcccc atattctatc tccattgtta tcaattaaca ttgcccaaac tttattttta
     1441 tgtagtactt ccttactaga atcatctaaa ataaactttc catcttttaa aatcaaatta
     1501 tcttcaatca taatcaagtt tgattttatt tctctgctat cacttgtatc taaattatca
     1561 gagtatttag atacaaatag catatatgaa aacagaagaa aacctgctat atttaataca
     1621 ataaaaagta atgaaatcaa gatacttgaa cctataaatc gcctcaatat gtactttaca
     1681 ctatccaaaa ttaaatcact cctcttgatt tcttttattc taatatctaa aattaattaa
     1741 tcatcaactt atatccaaga cctcttactg taataagatg tttaggtgta gatggagttt
     1801 tttctatctt ctctctaacc cttctaacat gaaccataag ggtattttca tatccataca
     1861 tatcatctcc ccaaatggct tgacatagtg aatcattagt aacaattctc cctttatttt
     1921 catacaactt taaaagcaac gaatgctctt tagctgttaa agaaatctct tgcccatctt
     1981 tctcaacaca tgcactattt aaattcacaa caacagaatc tcccagttta aatactggca
     2041 actcctcttc ttctataggc acatatacac gatttaatat acccacaagc cttaaagtaa
     2101 gttcctttgg taaaaatggc tttactatat aatcgtcagc ccctaatccc aaaccaatca
     2161 atctatcttc atcttcacct cttgcagaga gaaaaataac tggtatctta gatgtctctc
     2221 taatttttct cataatagag aaaccatctc catctggcaa tacaacatct aaaattgcac
     2281 aatctggctt tacatttcta aataccctaa cagcttcttc ataacttgat gctaaaaata
     2341 ctctactaaa tccctctttt cttaggattt catctatcat ttttaataat tccttgtggt
     2401 catcaaccac aagtattttt ttatctttta ttgactccat aatcatcacc ctcctttagt
     2461 ataccatata cccattttca caactttgcc taaatagact tttaaggtta ttgtaaggta
     2521 aacttcagct aactgaaatg taaccaagtt attatactga tataagttaa aagaatcaat
     2581 aagcacagat taaaaaaggg gatgaaacat atggaaatga taattagaac acaaagactt
     2641 tcaaaggaat acaataaaat atttagagta aaggatatag acctaagagt tccacaagga
     2701 gctgtatatg gttttttggg tcctaatgga gctggaaaat ctaccactct taaaatgctt
     2761 cttggacttg ctaaacccac acaaggaaat attaatattt taggtaaaga gttaaatgaa
     2821 aaaaatagaa tatcaatttt aaaagatata ggttctttga tagaatctcc atcatactat
     2881 ggtcatctta cagggcttga aaacatgacc ataatgcaaa gactcttaga tttacctaaa
     2941 aaaaatgtaa atgaagcatt aaaaattgta aggttagaaa atcaaaaaaa taaaaaagtt
     3001 aatcaatatt ctttaggtat gaagcaacgt cttggcattg ctatggcaat aatgaagttt
     3061 cccaaattat taatacttga tgaacctaca aatggactcg acccagcagg gattgaggaa
     3121 attagagaat taataaaatc tctaccagaa aaatacggca tgactgttct aatctctagc
     3181 catcttttaa gtgaaattga ccaaattgcc acatcaatag gaataataaa tcatggcgaa
     3241 ctggtttttc aagatagcat aaaagaactt cataatagag gtcaaagtca gattgctatc
     3301 aaagctaaga atgttgtatc tgcacaaaag ctattaaata ctaaaggata tatgtcactt
     3361 atacaaaatg ggtacttgac tattgaaaat ttaaaagact cagatgtggc taaaataaat
     3421 ggtatgcttg tccattcaaa tatagatgta tttagaatag aagaacataa aaaaagcttg
     3481 gaagatatat tcctagaact tactggtaag gcggtgactt tataatgaaa taccttggtc
     3541 tagaatttta taaacttaaa agaaagaaga ttattctgat gaccttttta tttgtagctg
     3601 ttgaaataat gtggtgtatc gtaaattcaa atagggctat tacacgtaat ccagatatgt
     3661 taggtggatt tgaatattcc tatatgctta tgagttttgc aagtttaaat ggtctattct
     3721 ttcctatctt aatatcaata attacatcac gcataagcga tatagaacat aaaggagata
     3781 catggaagct tttaaaatct tctgtgacat ctttaaatag tatttattta tccaaatttt
     3841 tatgttctgc tattttggta tctataccta tattgatgca agtgttatct attgtgttgt
     3901 ttggacattt tagaggggtt attgagtcaa tttctgtatc tttactttta aaatacacat
     3961 taggaacaat tcttgtaagt attgctatca ttgcactaca cctatggatt gcaactgtaa
     4021 ttccaaatca aatgattgca attacatttg gcatgattgg tagttttata ggtttgacat
     4081 caggtttgtt ttatcaaggt attcgtagat tgtttatatg gtcttattac ctagagttat
     4141 cacctttaag ttatgcttat gataatgttt taggttcatc tgtctataga attgatatga
     4201 atttttctat tacgatttta gttttcttaa ttggaatgct aatttattat attggaagaa
     4261 atcatttatt aaaaaaagaa gtataggagg gatttaaatg ttaaggaagt cagttttggg
     4321 agaaattcta aagttaaaaa attcttttat atggtatgta ttattagctt tgccacttat
     4381 aagtgtgttg attggttctg gtaattttta tctaaatcaa ggcatactca aaaaagaatg
     4441 gtacagctta tggactcaag ttagtctttt ttatggggaa tttttctttc ctatcttaat
     4501 cgcaatcttt tgtgcatata tttgtaggct tgaacatatg aatcataatt ggaataatgt
     4561 aatgacactg ccagttaatt taaaaaatat ctttttatct aaacttacag tagtttcaat
     4621 acttacagct attacacaag ttttctttgt tatcttttat atagttgcag gaaatatgtt
     4681 tggtttttct ggtagtgtac ctaaagaatt gactggttgg attgctaaag gttggattat
     4741 ttctatttca atagcttcta tacaattata catgtctata aagattagaa gttttgcaac
     4801 tcctattggt attagtcttt gcctttctct atttggtatg ggattaatag tagcaaaacc
     4861 tgctgttggt atatttttcc cttattctat gttaggtagt gctatggggg ttataaagca
     4921 atctgcttta aatgttaatg acactatagt tacaatatgt gttgcattta ttactactat
     4981 agtattttct gaaattgcca atagaaattt tagaaagaat gatgttattt cttaatggta
     5041 aatgctagat attttttggt ttgaaattta tattcttttt ggtttgaaat ttatatccct
     5101 tttatttaaa aatatatatc ttttatttga attttatatg ttttatctac tcactatata
     5161 atagatatac tcctaaaata aaaaaatata taataatgtt attatttaaa attattatgt
     5221 gttatattaa aacttaatgc aaaaacaatt tttatattat ataatgaaac ttaattaaat
     5281 atagctaaaa aatcattcaa tacattaaat aaatatttaa ctttaataat ctatatttaa
     5341 ttatggctat aacatagaat tatcaaatat attgaatgaa tatttgactc ttggaatgtg
     5401 taattaaatt aagtttggtt attcgatgga aggcaaattt gatataaaaa ctccatttaa
     5461 agtgccagat aaaaacttta tggttatata aaagttaaaa gtaatttata gtgttataga
     5521 atattcaaag ggatttaaag taatctat
//
LOCUS       DAEPLF010000216         5544 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEPLF010000216 DAEPLF010000000
VERSION     DAEPLF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.217"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(107..775)
                     /locus_tag="KR314_003120"
     CDS             complement(107..775)
                     /locus_tag="KR314_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH0329482.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMTEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     GKAY"
     gene            complement(768..1571)
                     /locus_tag="KR314_003121"
     CDS             complement(768..1571)
                     /locus_tag="KR314_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH0329483.1"
                     /translation="MKKLKLIIMLALVLALLITGCSSGNNKNKEEQKTIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMVEPPIQFGAC
                     SFAIQKNEKGEKWTKEINEVIEEMHKDGTLTKISEAWLGKDITKEPINE"
     gene            complement(1978..2400)
                     /locus_tag="KR314_003122"
     CDS             complement(1978..2400)
                     /locus_tag="KR314_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0329484.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGACPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            complement(2414..4591)
                     /locus_tag="KR314_003123"
     CDS             complement(2414..4591)
                     /locus_tag="KR314_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0329485.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALHSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANVMNIDDDLLKAGYEASIRHMFKKTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDNEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYRESDINSIIDDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            4872..>5544
                     /locus_tag="KR314_003124"
     CDS             4872..>5544
                     /locus_tag="KR314_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HBH0329486.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKRDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNIHYFADED
                     EKLVDI"
BASE COUNT         1855 a          932 c          604 g         2153 t
ORIGIN      
        1 ctatattttc tcaataattt agattactaa gtttattttt ctaaaattaa aaatttattt
       61 acaagtaata atatatctac atcatcccat ttttaaatat tgaaatctaa tatgcctttc
      121 ctattctttt ttcaactttg ctttgtatat tactcaatat tatagatact gcccagtaaa
      181 ttaatatcgt tatcatatat atctcaaaat atttaaaact tgaagcagcc tctacttgag
      241 ctactcccat taaatcttgt attcctatag ttagacctaa agaagaacct ttaattatgt
      301 ccagtgtaca gcttacaata ggaggtactg cagatgtagc tgcttgaggt agaattattt
      361 taaccattga ctgatattta cttaacccaa aagcataaca tgcttcctgt tgtcctttat
      421 caacactctc aatagatgct cgtataattt cagtcatata tgcagatgaa tttacaccta
      481 aacaaattac tattgctaaa taaggtgaac aatctttcat aaaagaaaat atcattggaa
      541 atccaaagta aaagaaaaat atttgaatta aaagtggtgt tcctctaaag aatgaaacat
      601 acactcttgt tatgtattgc aaaacttttg attttgtctg tgtgaaaaag gcaagtatta
      661 cacctaataa tattgataaa aaaaatgcaa caatagatat tagtattgtt attcccaatg
      721 ctttaatcaa aaatggaaat atacttagac aaaattcaaa attaaaatta ctcattaata
      781 ggctccttag taatgtcttt tcctagccag gcttcagata ttttagtcaa agtcccatct
      841 ttatgcatct cttcaattac ttcgttaatt tcttttgtcc atttttcacc tttttcattt
      901 ttttgaatcg caaaagaaca ggctccgaat tgaattggag gttctaccat tttaagattt
      961 aatttttgtt cttttatagc tattaaagcc tcaacatcct gcgcaactac tgcatctaat
     1021 cttcccattt ctagatcttg ataattccct ttaagagttg cacttgaata atttactatt
     1081 tttatttcat catttgggta cttttctttt agtatttcat ttgctactgc tccagtaccc
     1141 acacctactt tcttgccaac taaatcatct attgatttta tactagtatc atcttctcta
     1201 acaataatgt ttgttccact ataattataa gcatctgaaa aagcatattt tttctctctt
     1261 tcaggtgttg cagtaagttg agcagataca acatctgctt tatcagcttc taataatcca
     1321 aataatccac taaaatcagt taatacccat tcaactttac aattagttcg tttagcaaat
     1381 tcatcccaaa aatctacttc aaatccttta agtttatctt tatctttaaa agcaaaaggg
     1441 taatatgttc ctgatgttgc aactcttata gttttttgtt cttctttatt tttattattt
     1501 cctgatgaac atcctgtaat taaaagtgct aataccaagg caagcataat aattaattta
     1561 agttttttca taatttattc tctccttttt aatcttattt taatttttta tataatacat
     1621 tttcaaaggt tgaatcactt gtaccattat aattgtttaa tggctctgcc ataacataac
     1681 tcccaaatat tgatactgta gtaaaagatt gcctcttatt ttatacctta ctaaatttgc
     1741 ttttataaca ctataaatca attttctagt tattatatta tacttcgact ttgaacaaca
     1801 taagtttaat atgttttaca gtctttgtcc aatagcaaaa aactattact aaaaatcaca
     1861 ttatagtatc caactatatc cataaaaaag ctatattgag tttttattat aacaaactca
     1921 atatagcttt ttgtaaaaat atcttatacc aagtataagt cactctctat atttaattta
     1981 tttattaaat tttaatgctc ctgtaggaca taccattaca caaggtgaat ccataccaca
     2041 cttatatctc tcaatacaac cattacactt atttgcctta ccatcatttc cataaattaa
     2101 ggctccatat tcacaagctt tttcacaaag tttacaccct atacagtttt gattgtctaa
     2161 tacaacgaat ccttcttctt ttttaaaaca atttttagga catgcaccta aacattttgg
     2221 ctcattacaa tggatacatg ctaatgaaaa atattctata ttttcacctt cctctattgt
     2281 aaatacatgt ctaaaaagtt caccattgtc tatattatta tcattttggt ctatacatgc
     2341 aagcaaacat gctccacatg ctgtacataa ttctttatta aatttaaata aacttttcat
     2401 tatatctctc catttatcta ttcatttttt caatcttaca cttgcaactt ctatacgcag
     2461 ggaatccact ataagggtca aaattatcat caataatact atttatatca gattctctat
     2521 atccatgata catatgtata ttcttatata agactctatc agtaacattg acttttacat
     2581 ccaaacttcc tatcttagta gttattttta tataatctcc actgataaca cccaattctt
     2641 ttgcatccaa gctggaaata tcacaactag cttcattttt taatattctt gaaagtttga
     2701 tgtcatgaag tcttgaatgt agtgcatttg aaattcttgc tcctgatgtc aataaatgag
     2761 tatattcatt atctatatta tcattaaatc ctctatatgt tggtagtgca tccagtccaa
     2821 aatctttata tttttcaata attgtagatt ttaattcaaa cctagaagtg ctagtatcat
     2881 acccctctct tgtatatgag cctactatat atggcttaaa atctttacat tttaatggta
     2941 aatcactttc tttaagttta tctacatcta cacacgtctt tttaaacata tgtcttatac
     3001 ttgcttcata acctgctttt aataaatcat catctatatt cataacattt gctaaatctg
     3061 aaagtatttc tacatctgat tttgaatcgt ataatttatc aataactttt ttggtatatg
     3121 taatataccc tcctgggtaa ctcttacatt cttctctctc aaatgatgta cacgcaggta
     3181 aaagtatatc tgcatatttt gatgtatctg tcataaataa atctatattc acaaaaaaat
     3241 ctacactatg caacgcttta aaaagtctcc ctgaattagg aaacattcta gcatttatcc
     3301 catgacaaaa caaagctctt atttcatatg gtgtcttttc ttctatttgc ctaggtaaat
     3361 ccatagcttg aaattgagtt accatttcat tccatagagg aaatctttct ccaccaattc
     3421 tttcatttat atttttaggt agaacttctt ttacaaactc tttttctagt gtttggaatc
     3481 cacctatctt accagcaaag taatcttgtt ttggtatgtt tccacctatt ctatcataat
     3541 tacctgttat agcagataaa gacatcattg ctctatagtt ttgaaatcca ttttgatgat
     3601 gaactattgg agctgcactt tcatttatac aaaatggacc ttgtgacaac atctcaacag
     3661 cttttactat attatcttta gaaactcctg taattctact tacagtatct aaatcaaata
     3721 attttacata ttctttatat tcattaaatc catgtacatg cttttctatg tattctctat
     3781 ctattaaatc cttttgtata aatagattcg ccattccaag agctaaagct ccatcagttc
     3841 caggtttaat tcttaaatgt aaatctgcta gtttttgagt tgtcggtgtt attctagggt
     3901 ctacaattat aaattttaga cctttttctt ttaattttat tagattcaca ctatttaaaa
     3961 atttagagta gtatggatta aatccccacc ctaaataagt ttttgcattc tccatatctt
     4021 gcattgtaaa gtttcttcca gtagcaacat ttccagccat ctgtgctgat gttgaacatg
     4081 aactagattc tgtaccataa ttaggtgaac caaatgaaga tgcaaacctt tgcaaaaaag
     4141 gtctatacca ctttccataa cccgtaaaaa aagctacatg ctttgctcca tactcagatt
     4201 ttactctatt taatttttct gctatttctc tataagcttc atcccatgat attggtttaa
     4261 attttccttc acctcttttt ccaattcttt ttagaggtgt ttttattcta tcttctctat
     4321 atatatattc tctattgcaa agacctttgg tacataacaa ccctttattc attggatgtt
     4381 cttttgtacc ttctattttt attactttac catctttaac atatgcatca attccacaat
     4441 gtactcctgg agaacaaata tcacataaag tcctctttat ttctatacct gtttccttac
     4501 agggtatttt agctttaagt tgtctataac tatccatatt aacattttcc tttcccaata
     4561 ataaatacaa attattatac ttattataca ttctttttat tcaaatatca tactttatat
     4621 tttatatagt gcaatttcta agaatatttt taataataag attttatatt tattaataat
     4681 ctatttatat aattattttt cattcatagt ttataagata attcaataaa aatctaatag
     4741 taaattgtta tttacacata tattgtaata gataaaagct attcatatct gccttaattt
     4801 tagtcttaaa tttagagtgt aaattgaatg catttagatt ataatatata cagtgaggta
     4861 aggtgaataa tatgaaaata gatatgatat ataaactaat aagaagaaat acagaaccat
     4921 caagatggtc aagaggtgta aaatattata ctaatcaatt agttgattat atagacatag
     4981 atattgatga cctacaaaat aacctgttga catttcgtgg tggagtcaat tctgaagata
     5041 attatagctt ttattttaat tcaataacta tagatataaa gcactttaag ataattgaaa
     5101 atgattgtag ttgcgctgac tgtcaatcaa agggcaaaag tatttttata tgtaagcata
     5161 tagttgctat tgtgataaaa agcttaaaac aaataaaaaa taaaagagat gttcctccta
     5221 ttctaataga tatattaggt gcagataagg ataagctttc taatacaaat tctaaaacaa
     5281 gcttagattt aagcaagcaa ttactttctt tgttagacaa tactaaaaga gaaagggtta
     5341 atttagatgt attgataaat gtggattcaa ataaagcaat tgctgaattt aaaataggaa
     5401 ttgataaaat gtacatttta aaaaacatta ctgaatttgc atattcaaaa cttaatggat
     5461 atgaattaga ttttgggaag aaatttactt acaaccccaa cattcattat tttgctgatg
     5521 aagatgagaa gttagtagat atta
//
LOCUS       DAEPLF010000217         5530 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEPLF010000217 DAEPLF010000000
VERSION     DAEPLF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.218"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..1602)
                     /locus_tag="KR314_003125"
     CDS             complement(<1..1602)
                     /locus_tag="KR314_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBH0329487.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDSKPFDKFQFVRNGFFSIDNKYTT"
     gene            1894..2607
                     /locus_tag="KR314_003126"
     CDS             1894..2607
                     /locus_tag="KR314_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBH0329488.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            2766..3329
                     /locus_tag="KR314_003127"
     CDS             2766..3329
                     /locus_tag="KR314_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329489.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            3726..5426
                     /locus_tag="KR314_003128"
     CDS             3726..5426
                     /locus_tag="KR314_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329490.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
BASE COUNT         2058 a          687 c          871 g         1914 t
ORIGIN      
        1 agttgtatat ttattatcta tactaaagaa tccatttcta acaaattgga atttatcaaa
       61 aggtttagaa tcttttatct gtgtaggttc aacaaaacct tgtaatattt ccaatgaatt
      121 aggatttatt tgttctaaga aatgtttacc ttcattttca ggtatatcat caagaattaa
      181 tggttcaaat aatctaaatt cacatggtat agctgattta gcatcaaccc aatgtattgt
      241 agattttacc ttacgtcctg taaagccaga gccactctta gtttcagggt cataagtacc
      301 atgtatttca actacatttc cattttcatc cttaattaca tcagtacatt ttacaaaata
      361 agcacctttt aaacggactt catttcctgg gaaaaatcta aaatatttct taacaggttc
      421 ttccataaaa tcatcttgtt ctatatacaa ttctctagag aaaggaacta atctctttcc
      481 ttgagattca tctttagcat tattctctat ttcaagcatc tcaatcttat cttctgggta
      541 attagttata ataagtttta aaggtcttaa aactcccata gtaagaggag cctttggttg
      601 taagttttct cttaggaaat aatcaagcat ttgactatct accgtagagt taacttttga
      661 tacccctatt tcactacaga aattgcgcaa tgcttctgca gtatatccct tacgtctgat
      721 tccggatatt gtaggaacac gagggtcatc ccaaccatca acaataccct catctacaag
      781 ttgttttaat ttccttttac tcatgacagt attatttata ttaagtctag caaattctat
      841 ttgtctaggt atattttcca tctcacattc tttaacaaac caatcataaa gaggtctttg
      901 gtcttcaaac tctaaagtac atatagagtg agttattcct tcaattgcat cttcaattgg
      961 atgagcaaaa gcatacatag gatatatgca ccatttatct cctgtattat ggtgtgttga
     1021 atgagatatt ctatatataa ttgggtctct aaggtttata tttggagaag acatatctat
     1081 tttggctctt aatgtttttt caccatcttt aaattcacca tttttcattc tttcaaataa
     1141 atcaaggttt tcttcaatag ttctgtttct atgaggactt tcttttcctg gttcagttag
     1201 agtaccacga tattctctca tttcctcttg tgtcaaatca catacataag ctttaccctt
     1261 ttttattagt attaaagctc tcttatacat ttcatcaaag tagttagatg caaaatttaa
     1321 ctcgttccaa tcaaatccta accactttac atcttctttt atggactcta catattcaac
     1381 atcctctttt aatgggtttg tatcatcaaa tcttaaatta gcttttccat taaactcctt
     1441 agctaatcca aaattaagac atatactttt tgcatgacca atatgtaaat atccgtttgg
     1501 ctcaggtgga aaacgagtta tgatactgtc atgttttcct gtttctaagt cgtttataat
     1561 aatattcttt ataaagttag atgagttcgt ttcatttgac atattctata cctcctattt
     1621 aatttgatta taatacagaa cactacactg tattgcataa tattaatttt aatccaagat
     1681 tagaaaaaaa taaaggtgta attaagagta ttatgcaaaa tattaacttt tatactaaaa
     1741 acatgttttt acagtttatt tacaaaacat tagattggtt gaattaattt ttaaaagact
     1801 gtattatagt atgtatattg ttaagaatat tatgtgaagt tagttatcta aatttatttt
     1861 aaatctgatg acagatttgt aaaggagacg attatgggaa aggttgcaaa gattttgtta
     1921 agtttaattg ttattgtttt agtacttgca ggaattgttg tatatatctt gacaccaaaa
     1981 gaaagatatg atgttagttc acaaaataat aaaatcatag atgaagaata tttatcaaaa
     2041 ggctcatata taaattctat ggaggtagta aaaaatccac ttagaatggt tggaaaaata
     2101 tctgtttcag aggaagagtt taaaaattta atatatacat tgatgcagaa gcatggaatt
     2161 aatgaacttg aaaataattt tgttgaaatg aaaaatggta aaataaaggt agcaggacca
     2221 tataaggtat ttggtttggt aaatagtcaa tatgaacttg aattgagacc tactttaaag
     2281 gatggaaact tagttgtaag tcttgagaat gtgaaaattg gaaaatttaa actaagtgat
     2341 aaaatgctag aaaaaatatt aagtaactac aataaaaaag ttccatttga ggttaatgga
     2401 aataagatta ctttggaaaa aagttatctt tacccaataa cacttaaaaa tatatctata
     2461 aaaaaaggaa atgttgattt ggatatggaa gtagaaatag atttaaaatc acaaataaat
     2521 ggggcattag gatatttaaa ggactctgga aaggttcaag atatactaaa tcatctgatt
     2581 aatttgaaga aaacatctgt tggttaaaaa tatagaaaac ttatatttat atttttatta
     2641 tattatataa tattaaagta ccttaaaatc aaaatttgta aaaccacatt tattatgtta
     2701 gaatatctta tgatattaaa ttgtaagtgt ataatatcat ttagttacta gattgggggg
     2761 aaatgatgaa taaagtctat attgtaacaa catatacagg aactatatta tcttacttaa
     2821 taaggaatat aagtaaaaag ctgtatacac atgtgtcaat atcattaaat gaaaatctaa
     2881 aaccaatgta ttcttttgga aggctaaatc caaggaatcc atttattgga ggatttgttg
     2941 aagaaaatat aaatcaagga ttgtatgcta taagaaaaaa cactgtgtgt agggtatatt
     3001 cgttagaagt agataattta caatatgaaa atctatataa aaatataaaa ttaattagtg
     3061 attatagaga agattactat tacgatacaa tggcattaat atatttacta atgaatattc
     3121 agagacaatc tgaatataaa tatgtatgtt caaactttgt tgctgacatg ttagaaaaaa
     3181 gtgagataga cattttaaat aaacaagcat ttgaagtaac gccaaatgat ttttataatt
     3241 tatctggctt aaccttagag tatgaaggtt tattatcaga atataattct agacaacatt
     3301 cttatgtatc aaaaatcgca aacatttaaa aacttgattt agagtttttc atataaaaaa
     3361 atattgctag ataattaggc tgaagatagt agtttaactt cgataggatt aagaatttgt
     3421 aaatcttagt tagaatactt tcttcagttt ttaaatttct gctaatattt gtatttttat
     3481 aatatttttg gatatatata atacagaata gtgcaaaaat cccactcctt taggtgcggg
     3541 atggatagca caaacagtgt ataaaaagta tttatacata cacgtataat gttaaatata
     3601 gttgaataag cagaactata aatatataaa gatatcttaa tatataataa atatgataaa
     3661 tatttatttt ttgtaaatat atggatataa taataataat aacatttttt agagaggagt
     3721 attaaatgaa acgatatctt aaaacatatg gagttgcatt aataattgta ataatttatg
     3781 catttattaa attacctgta ctaagattag attttacatc tttattttca gtaggaataa
     3841 ttttctttgt tgtagcagga atactagata tgatgttaga tagaaatgaa agagcatcta
     3901 aaatggctaa atataatttt tcaatagcaa tagctttgct tgtgtatatt gtagtagttc
     3961 cctttataac ttctacacct gtacttcatg caaaatcata tagagaacta ttgggaaaag
     4021 taactgaaag taaatttact gatgacatta gccctgtcag tgtaaatgac attcgtcttg
     4081 tagatgaaga tatggcaatg aaattaggtg acaaaaagct tggagaagtt cctgcaatag
     4141 ggagtgtatc caaattagga aaatttcata tacaaaatgt agatggagag ttgtactggg
     4201 tagcgcctct tgttcacaga gacataatta aatggataac atctctaagt ggtacaagtg
     4261 gttatgtaaa agtatcagct agtaatcctc aagacgtcca attagttcaa gaaatagatg
     4321 gaaagccaat aaagatagtt taccaaccag aagcctactt acatcaggat ttacaaagac
     4381 atttatacat acatggtatt gtaaatgttg gtatgacaga tttcactctt gaaataaatg
     4441 atgaaggaag accttattgg gttgtaagtt tatatgaaca taaaattggc tatggaggag
     4501 caaatgcaac aggaatagcc actgttgatg cagaaactgg aaaaataaat gtttatgatg
     4561 ttaaaaacac acctaaatgg gtggatagaa tacaaccaca aagctttgta acagaccaga
     4621 ttaaagactg gggtgtttat gttaatggat ttttaaactc tgttatatca gaaaagggag
     4681 ttttagtacc aacagaaggt acatcattag tttatggcaa tgataacagg tcttattggt
     4741 atacaggtat aacatcttca ggtggagatg aatctacaat tggatttatg ttagttgatt
     4801 ctagaacaaa agaagctaga ttatataaac aaccaggagc tactgaaacg gctgcaatga
     4861 aatcagctga aggtaatgtt caagaaaaaa attatgaagc gacgttccct gtaatgtata
     4921 atatattggg tcagccaaca tatgtatcat cattaaaaga taaggctggt cttgttaaga
     4981 gagttgcatt tgtatctgtt gaagattata atgtacttgg aataggtgaa gataagaatg
     5041 aagcattaag aaactataag gatgcattag aatctaaggg taatgattta aaacttgata
     5101 atgatttaaa agatgaggta ttagaaggca cagtgacaag aataagccca gatgtgcgag
     5161 gtggtaacac taattattat gtaactttag attcaaataa agatataata tttagggcta
     5221 cttcaaaagt atctagcgaa ttgccattga ctcaggttgg tgataaagtc aaaatatcgt
     5281 attcaaaaga agaatctggt gtaatagaaa tgtcagaatt tgataaccta aatattgcaa
     5341 actttgaaga taaaaaagca aaagaaaaaa caggaactga tgaaaataca gattccaaag
     5401 attctgaaaa tcaaggtaat gtataattta aataaaaaga ttgataatag tatttaacat
     5461 agtgatagct aatttttata gaatttaatt ctataaaaat tagctatttt attttaattc
     5521 tttgtaaatt
//
LOCUS       DAEPLF010000218         5445 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEPLF010000218 DAEPLF010000000
VERSION     DAEPLF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.219"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..1080)
                     /locus_tag="KR314_003129"
     CDS             complement(<1..1080)
                     /locus_tag="KR314_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329491.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCDIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKE"
     gene            complement(1202..3745)
                     /locus_tag="KR314_003130"
     CDS             complement(1202..3745)
                     /locus_tag="KR314_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBH0329492.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGAKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQAKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(4023..4357)
                     /gene="rnpB"
                     /locus_tag="KR314_003131"
     ncRNA           complement(4023..4357)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KR314_003131"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            4489..5166
                     /gene="srtB"
                     /locus_tag="KR314_003132"
     CDS             4489..5166
                     /gene="srtB"
                     /locus_tag="KR314_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBH0329493.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKTSSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKNNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(5326..5400)
                     /locus_tag="KR314_003133"
     tRNA            complement(5326..5400)
                     /locus_tag="KR314_003133"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5366..5368),aa:Thr,seq:tgt)
BASE COUNT         1665 a          916 c          523 g         2341 t
ORIGIN      
        1 ttctttatta attacaatgt cttttatatc actatttttt atagatatga tttctttttg
       61 aaaagacttt agaatagtca agctatcgaa atcttcttga ataaaaaatg gagcatgttt
      121 taatgtatat cccactattt cattttctat atcttttaag tcatctattt gcataatttc
      181 tctattttta tcatttatat gtaggttaaa tacattttta ttatcatata atattatatc
      241 ttctcctaaa tttaacttag aacaattatc tttcgctgac tttacaatag tcttcttaga
      301 tttcttattt ataaaaatta attggtcttg atattttact aaaagcatgt ttgtgttttt
      361 ataatcaaat ccatctatat ttcctgatat cttgcagaat actatatcac aatctttagg
      421 aagatttcct attttttggt tatctttaac tctctcattt aaatcagatg gaactacact
      481 atctatattg ttattaaata tagatacaga ccttactata atcccctctc tttccatatg
      541 aaagtatccc ttcaatgaca ctcttttatc ttctaaaatg tatctttcta tatcatcaaa
      601 atttatttca aatctatttt tcccactgat aaatataaac ttctcattat ctttattaat
      661 tccacccata taaaacttat tgtcatctaa acttaatata gtcatattat cctttagata
      721 gaatataaat tctctttgac ttttgcagat taaatcatat tcatccaaag ttattttcat
      781 agtcaaattt gctgtaacat ttttacttga attttcactt tttatgtaaa aaggcaagtt
      841 atttccatag acaattattt gagaatcttc taaaactgcc tctaattttt ctttattgtc
      901 tgaaaaacta tatatatatt ttacacctgt atcctcatta acttcatctg atttgctgat
      961 tattatattc cctagatttg ttatatgaag ctttttatat tcttttgagt atctacttat
     1021 gccactaagc tcaaacccat cagaaagctc atatatgatt ttttctccta atttcaacat
     1081 accttcacca cctaaacaga attataagta ttttataaag gtgaataaaa tgtctcaaaa
     1141 caaataatat ttttttaata ctttttattc tgagacatag tttctccttt tttataatgc
     1201 tctaagcacc ttcaactatt tcttcataaa gtttatctaa atgtttaaca tattcacgat
     1261 atccagattt gttctttaac tcacttctta aatcttcata tttttgttta agttcttcag
     1321 cactcaaaga aggagctgat tcttttaatt cttctaactt ataatcatag tacttatcat
     1381 acatagctat atatgtatca atagatttct tagctagttc attacatttt tcttgtgctt
     1441 ctccatattg agaataatca cctattttta ttttatagta tccattttct tgaattattg
     1501 ttgaagaaat acctttagcc tcatattctt ttgctgtttc ttttgctgtt ttttcaactt
     1561 taagtgaacc tgcataaaca gtgtaatata cacctttacc atctttatat gcttccattt
     1621 cttttgcttt cttttcagct tgtgcttttt cttgagcctc tttttcagct tttgcctttt
     1681 cttctgcttc ttttttctct ttttcagctt gtgctttttt ctcttcctca gttaatttat
     1741 tgttagcttg ttgttgagtt gatgactttg gaatattctc tttatcattt ttcttattgt
     1801 tgcctactgt tataaaatat cctacaccaa caataaccac tataactact gctataatag
     1861 tttcttttaa tctactcttc ttagaaacaa cctcttcatc atctaaagaa tcttctttat
     1921 ctgatacata atttttcatt tctttcttgt tttctctata tttgtcctta gaagaatttt
     1981 tagatttttt attactattt tctttctttt taataggtat tactactgat ttttcatttt
     2041 ctaatttttc ttcttttatt tcttttgctt ttttatcttc taagttttta ctttcttttt
     2101 caagcttcga ttcattattt gaagatactt tttcatctaa gttattattt attggttttt
     2161 ctatttttac actacgttct tcttctatat gttgacttgg ttcttcttgt tttatttctt
     2221 ttaattcatt ttgctctatt tcttctgatt ttgcttgatg attttcaact ggtttatcta
     2281 aaattatatc aggagaaata tcatttaaat tctcttcttc ttgttcaact tcttcattgt
     2341 cttttttaat aaatccaaat aatttagcct tctttttact ctttttattt tctggttcaa
     2401 ttatatcttc tttattgctg tcgtactctt tatctgtagt atctttatct tctacaacgt
     2461 cacctatatc ttgattatta ctatctactt cttctttagc cccttcttca gcatcatctt
     2521 ctattgaaat actacttcta aagcaaatat ctttatctgc attttttatc atgtaaatgt
     2581 attcttcaat atcgatatct ttatttcttc tagttctttt tgatgcaatt tcatgatttt
     2641 ctaattcact atagttatct tttattagat tctgtgcttt attccatgta tcttcactaa
     2701 tttctgtttt atttttaatt acatagtcca caaattcctt aaaattttca ggaccaaatt
     2761 ctccaattat agtcagtctc attgcacctt ctataaggtc tctatttctt ctctttaaaa
     2821 attctaatac aaccgttgca caagcaaaat ctttaataat ttctttgcca aaaccatatc
     2881 cttcaacaga atgaagttct tctaatccac ataacttctt atattcattg tgtattgttt
     2941 gagatatact ttcattattt aagctattta acttatctat aaacttcgat tctatatcat
     3001 ttgattttga ttcatgtttt acttttgaat ttttaatttc atttagtagc atgtcatttt
     3061 ctgaattatc tttacctaat tgcaaatcct caatttcagt tgcttttatt tctactttgt
     3121 cttgttcgtt attctcttcc acattattag gtaccacatc tttattgcta gctaatattt
     3181 tgtttacaca gaatataatt tctcttttta attctctttt cgcatctgct tgatttttaa
     3241 gaaataaatc tccacattct ttcttttcta aacttatttt tctaagagat atattaagga
     3301 ggtctttata taggtttaaa accttataat ccttttctaa acgtcttctt ctctctgata
     3361 gttccatttt atcataagct aaaaaactat ttaaatcatt ggcttttaga gagtctaatc
     3421 cctctttcat agccttattt acatactttc tacattttgc tgtagttaat tctaaatctt
     3481 tttctaattc atctatttca ggtatgtact tatctactat ccatttgcca ataagtggtt
     3541 ctgtatttct tgctaatgtc ttataaaaca atatattaaa ttttaaaagc tctgcatatt
     3601 ttgcttcatt aaattctact ttttcaaatt tcttttccaa aacaccatct agcttttcat
     3661 ttattttaga ctcttgtttt aaaaaacttg ctgtgacatt taaaacagtg tttcttatta
     3721 tattattttc actcaaaacc ttcactcctt aaatcaatta ttttattgta atatttacat
     3781 tgtgcaaagt tgctaatttg attatgatat ctaaataatt atttactaaa tttgctacat
     3841 cttatatcat atcatattat ttacatcatt ttactttttc tttttatatc tatcttatat
     3901 tataccattt ttaatatctg tactaactta tattacaata ttcctcaaaa ttttaaaagt
     3961 agattagaca aatttacaaa taaaaaaaca tcagtttaaa ctgatgtttt tgactatcga
     4021 aataattaag caagtctata agcggagttc tgtattagat aatcatcttt ctaggcctat
     4081 tgtcaccaat aggctcaagc gacctaccta ccggagagta cgagcagcac tctttattcc
     4141 tatcttggtc ttgctccagg tggggtttac atagccaact agtcgcctag ctgctggtga
     4201 gctcttacct cgcctttcca tccttaccac aatagtggcg gtttatttct gttgcactat
     4261 ccttaggatc gctcccacta ggcgttacct agcaccctgc tctatggagc tccgactttc
     4321 ctcatgtaag attacatgcg attatctggc ttacttaata tttattttac ttagttatgt
     4381 taacacaaat tcttttacat gtcaaagtaa tattatacta aaaatttaaa tttattcaaa
     4441 aatagtgtaa tatagatata taaactccat ttaactcgag gtgatatatt gaaaaaatta
     4501 tatagaatag taattaacat tatactagta ttagtaatac tttacagtgg atttaatata
     4561 tactcaaaac ttaccaaata caatcatgat actaaaacat cctcagaact tcaaaaaaaa
     4621 gaatataaaa aagaggattt atcaaagatt aacagtgatt ttaaattttg gctttctgtt
     4681 gagaatacaa atataaatta tccagttgta caatctaaag ataattctta ctatcttgac
     4741 aaagattttt ataaaaaaga ttctatttct ggtacactgt ttatggatta tagaaataaa
     4801 tctattgatg acaaaaatat cataatatat ggacataata tgaaaaataa aactatgttc
     4861 aacaacctaa ataagtttaa agatgctgac ttctttaaaa acaataataa aataaaaata
     4921 accttgaatg gaaaagaatt tttatatgat gttttttctg cttatattgt agaatctgat
     4981 tatgattatc taaaaacaaa ttttaacaat gaatctgatt atcaaaatta cataaatgat
     5041 attacttcta aatcattgta taaatctcct attaaggtaa atagcaatga taaaatagtt
     5101 acactttcta catgtaccta tgaatttgat gatgccagaa tggtgattca tggtagattg
     5161 atttaaaact cacagacaca gtttaaaaca ataattttaa actgtgtcat cttttatacc
     5221 tataatttat aaattaggcc actaaaagta tgtaatacaa aataacaaat acatcaaaca
     5281 ataaaaaaca ataaaaaaag actatgctaa aaacatagtc tttactggag ctggtgatag
     5341 gaatcgaacc tacaacctgc tgattacaag tcagctgctc taccgttgag ccacaccagc
     5401 acacctattc tattgtaaat tagtaaaact aatttttaaa agtta
//
LOCUS       DAEPLF010000219         5442 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEPLF010000219 DAEPLF010000000
VERSION     DAEPLF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.220"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            796..5307
                     /locus_tag="KR314_003134"
     CDS             796..5307
                     /locus_tag="KR314_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBH0329494.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDIINQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGIGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADVEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWN
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVINLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNLNNNTSTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVINAGPSTAQNVTL
                     TDNIPASILSPEYSIDNGVTFQPWNGSLSIGTLGAGEIRNIIIRGIVSQTAIGTIINT
                     ATVSSTTPDPNLNNNTSTSEIDISSSADISVIKLANKTEACVEEQIDFVIVVSNAGPE
                     NAQNVTLIDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTC
                     LDRLTNIAEVTSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCC
                     NCCKNDFWC"
BASE COUNT         2111 a          800 c          909 g         1622 t
ORIGIN      
        1 tccatgatta aatcctcttt gtatcttata ctaatataat gaatataaat aaaatattta
       61 taatactaat ataaattgat ataaaaacaa ttacaatatt atattgatta ttcaaaatta
      121 tatataaaat tcagaaaaac atttcagttt acataataaa aataaagtaa actaaaatta
      181 ttgattttag attaaagtgc cagattaagt atttaagtag attttaagct tgatttgatt
      241 agaaaggtac aaactatcac cgaaaaaaat taaacccatt taaaaagcaa tataaaagct
      301 tgttattttc gagaaaaata gaccctctaa actcaattag ataaaaataa aatacaaaaa
      361 attatatctc tgaatatctc aagtaattaa gtttaaattt tacgatattt tgtttgatga
      421 tttataatat aaaagcatat tcttaatctt ctcaaattaa aaagagtaaa aaataacaaa
      481 aaaatcactg acatttcact gacaaattgt aattttgtta ttataaatac attgaaaata
      541 cttgattatt gaaaaatggc ataaaataat accttttatg ccatttacta ttgtttgata
      601 gtttttaact tatgacataa gatatttttt aacatataca ataaaatata aaaaaattga
      661 tattgcaaat aaaaaaaata catattagat gttatagaaa aaaagcgcac ttataggctt
      721 aaaatgtcac ataaaagagt ttcaaagaat aatatattat gaacacaaat tttgtaatca
      781 caaaggagtg ataatatggc aacagtatca ggacaaatag tttttgatag aaatagaagt
      841 gctaccattg attctgggga ctcaggaata gccaatatac cagttgtatt acaagatata
      901 gcaactggag ttagacttgt ggttctcaca gatgcatcag gaaattatgt ttttactaat
      961 gttccaaatg gtaactatag aattgtagaa gcttttggaa ctacaggagg agtaccaaca
     1021 ccagggaatt ttgcaacagc gatagtaggt ccagttccaa ttgcagaaat gccacctatt
     1081 agttctgtga caaatcctcc tattggttca acaaacttag atggtacaac acctaacaca
     1141 ctattaataa cagtagcagg ttcagatata ataaatcaaa atatattaaa tgggcctgtg
     1201 atttatacac ctatagagaa tatattggat tcttgcgtgt ctgtatctaa tataaacctt
     1261 attacagatg cagataacgg aacattcggt ttttttccac caggtacacc aagtaataca
     1321 ggacctgaaa tagaacctta cccaaatgtg acgccagatt ttacgtatgt tgtaccagac
     1381 ccaactaagt ttacaccttt agatggagaa tatacagtac aaaatattat gacaaatgca
     1441 atgagtaatg ttataggagc ttggtggcgt attgctgacc atactacagg aaatgaaaca
     1501 ggaagaatga tggtggtaaa tggttttaat cctggagctg tattttttaa atcaacagta
     1561 caagttacac ctaacactaa ttatttattt agcacttgga ttttaaacct ttttaaggta
     1621 acaggatatc caccaccaca gctaggtgta agaatatttg accaaaataa taatatacta
     1681 tatcaagcaa cattaggagc tcaaattcca gttaatacaa acgcaccaga atggaaagaa
     1741 gtaggtactg tgattaattc tcaaaataat aatcaactta cagtagaatt ttttagtgaa
     1801 ggagaaagcg ccattggaaa tgactatgct atagatgatg tttctttaag agaaatagct
     1861 gttccaacct ttactccaat aaaaacaagt aacacagaaa ctgctaatgt aggtgatata
     1921 atcacatata cagttacatt agataatact tgtgatagcc ctttaactaa tgtatttttt
     1981 agggatttaa ttccaaatgg tttagagttt atacctggaa ctgtaacggt tgatggagta
     2041 tctgtaccag gagtagaccc taatacaggt tttccacttc cagatattgg aggaggaata
     2101 ggggttgaag taacttttga tgttgtggtt gaaagtattc ctaacccaaa tcctactaat
     2161 aatatagcta ctatagatta ctcctataca cctgtagaag gcggtatacc aaatgatttt
     2221 tcagtagatt ctaatccagt tcctgttgaa gtaatctcag cagatgttga agttacaaaa
     2281 ttatctgaac ctactatagt gaatccagga gaggaattaa tatacactat aaaagtagta
     2341 aataatggac cattcccatc tgagaatgtg gttttaacag atgatgtacc agcttctatt
     2401 ataaatccag agtattcatt agatggagga gtaacttttc aaccatggac tggaagttta
     2461 aatataggga cattagaagt aggagagaca agagttatta taattagggg tattgtaaat
     2521 ccatcaacag taggaattat aacaaataca gctgtagtat cttcaactac agcagaccca
     2581 aaccttaata ataatacatc tacaatagag acagaagtga atttattagc agatatttta
     2641 gtaatgaaaa cagcagaacc taattcagct gttccaggta ctttattgag atatactatt
     2701 caagtagaaa acttaggacc agcaaatgct gaaaatgtaa tattaaatga tgatattcca
     2761 gcctctatta taaatccaga atattcattg gatggaggag caagttttca accatggaat
     2821 ggaagtctta atataggaac tttaaactct ggaatttcat taacagtttt aattcaagga
     2881 acagttagtt taaattcttc agaatatata gtaaatacag caacagtttc ttcaactaca
     2941 cctgacccag atttaagcaa caatatatct accattatta caccagttaa cccacaagca
     3001 ggtatttcta ttataaaagt agctgacgag gatgtagctg ttccaggaga agaatttgta
     3061 tatacaattg aaatttttaa tgaaggacca tctaatgcta cgaatgtagt tttaacagat
     3121 gatataccag atgttatttt aaatccagaa tattcattgg atggaggagc aactttccaa
     3181 ccatggaatg gaagtcttaa tataggtaca gttgcaccag ggcaattaat aagaattatc
     3241 ataaagggat tagtaagttc aactgctaca ggagatatta ctaatacagc agaagtaagt
     3301 gcagatgtac cagaaccagt tacggattca tcaacagtga caactcctat agttccatca
     3361 gcagatatag aagtaataaa aactagcaat atggatacag cagttccagg agaaactttt
     3421 agttatacta taacagttat aaatttagga ccatctgctg cacaaagtat cgttttgaca
     3481 gatgatatac cagatgttat tttaaatcca gagtattcat tggatggagg agtaactttc
     3541 caaccatgga atggaaatct tagtataggc actttggatg ctggagaaat aagaagtatt
     3601 ataataagag gaacagtgag ccagacagca gtaggtacaa ttataaatac agcaacaata
     3661 agttcaccta cacctgaccc aaatccagat aataatactt caacagatga aacagatata
     3721 agtccattag cagatatttc tgtgataaaa ggcaacgaac cagttgctat accaggggga
     3781 agatttattt atggaattga aattgcaaat gcaggaccat cttttgcaga gaacgtaact
     3841 ttaacagata atataccagc ttctatttta aatccagagt actcaataga taatggagtg
     3901 actttccaac catggaatgg aagccttaat ataggtactt tagatgctgg agagataaga
     3961 aatatcataa taaggggaac agtaagtcaa acagcaatag gtacaattat aaatacagca
     4021 acagtaagtt caactacacc tgacccaaat ctaaataaca atacttcaac atcagaagca
     4081 gaggtaagtt catcagcaga tatttctgta gtaaaacgaa gcaatcagac tgttgttgta
     4141 ccaggagatg tactagacta tacaattgag gtgagaaatg caggaccgtc tactgctcag
     4201 aacgtaactt taacagataa tataccagct tctattttaa gtccagaata ttcaatagat
     4261 aatggagtga cttttcaacc atggaatgga agtcttagta taggcacttt agatgctgga
     4321 gagataagaa atattataat aaggggaata gtaagtcaaa cagcaatagg tacaattata
     4381 aatacagcaa cagtaagttc aactacacct gacccaaatc taaataacaa tacttcaaca
     4441 tcagaagcag aggtaagttc atcagcagat atttctgtag taaaacgaag caatcagact
     4501 gttgtcgtac caggcgatgt actagactat acaattgagg tgataaatgc aggaccgtct
     4561 actgctcaga acgtaacttt aacagataat ataccagctt ctattttaag tccagaatat
     4621 tcaatagata atggagtgac ttttcaacca tggaatggaa gtcttagtat aggcacttta
     4681 ggtgctggag agataagaaa tatcataata aggggaatag taagtcaaac agcaataggt
     4741 acaattataa atacagcaac agtaagttca actacacctg acccaaatct aaataacaat
     4801 acttcaacat cagaaataga tataagttca tcagcagata tttctgtaat aaaattagca
     4861 aataaaacag aagcatgtgt agaagagcaa atagattttg taatagtagt atcaaatgca
     4921 ggaccagaaa atgctcaaaa tgttacttta atagacaatg tttcagataa gcttaagaaa
     4981 gcagtatttt cacttgatag aggtgtaagt ttccaaccat ggactggaag tcttaatata
     5041 ggtacactac cagctggaac tttaagagtt attctattaa gaggaattat aaaatcaaca
     5101 tgtcttgata gattaacaaa tattgcagaa gttacttcaa caacaccaga ttcaaacctt
     5161 aacaataata tatcaagagt gcaagtagaa ataaaacaat gttgttgtaa tgattgctgt
     5221 tgtaacaact gtagtaattg ttgttgcaat gattgtggtt gtaaagattg ttgctgtaat
     5281 tgttgtaaaa atgacttttg gtgttgattt tataaaataa ttttgtatac cttaaattaa
     5341 actgaaataa aaaataaatt cacatatgag taaaatattt tataaatatt aataaatttt
     5401 ctatctttga tatggaccac attcgtaata ctagttatta aa
//
LOCUS       DAEPLF010000220         5426 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEPLF010000220 DAEPLF010000000
VERSION     DAEPLF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.221"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            34..258
                     /locus_tag="KR314_003135"
     CDS             34..258
                     /locus_tag="KR314_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329495.1"
                     /translation="MKYSKGFKLIYSLVEYSKRFKLIYDVMKYSKEFKLIYSLVEYSK
                     RFKVIYSVMKYSKRFKAIYNAVKYIQGDLK"
     gene            470..739
                     /locus_tag="KR314_003136"
     CDS             470..739
                     /locus_tag="KR314_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBH0329496.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIGQCRKWLEETVSN"
     gene            complement(956..1201)
                     /locus_tag="KR314_003137"
     CDS             complement(956..1201)
                     /locus_tag="KR314_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBH0329497.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            1738..2298
                     /locus_tag="KR314_003138"
     CDS             1738..2298
                     /locus_tag="KR314_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin regulator"
                     /protein_id="HBH0329498.1"
                     /translation="MFFGNISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAE
                     PWFKMKDDEKKAIEAENQRKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYK
                     YKKVKFEGKVIQVIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGI
                     SAGTITYESTMGGKITIPGIAVEKIN"
     gene            complement(2633..>5426)
                     /gene="tcdA"
                     /locus_tag="KR314_003139"
                     /gene_synonym="toxA"
     CDS             complement(2633..>5426)
                     /gene="tcdA"
                     /locus_tag="KR314_003139"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBH0329499.1"
                     /translation="DFKDIKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYD
                     EDSKLVKGLININNSLFYFDPIESNLVTGWQTINGKKYYFDINTGAASTSYKIINGKH
                     FYFNNNGVMQLGVFKGPDGFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDS
                     KAVTGWRIINNEKYYFNPNNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYF
                     DTDTAIAFNGYKTIDGKHFYFDSDCVVKIGVFSGSNGFEYFAPANTYNNNIEGQAIVY
                     QSKFLTLNGKKYYFDNNSKAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTN
                     TAEAATGWQTIDGKKYYFNTNTSIASTGYTIINGKYFYFNTDGIMQIGVFKVPNGFEY
                     FAPANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAITGWQTIDGKKYYFNPNNAI
                     AATHLCTINNDKYYFSYDGILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFA
                     PANTHNNNIEGQAIVYQNKFLTLNGKKYYFDNDSKAVTGWQTIDSKKYYFNLNTAVAV
                     TGWQTIDGEKYYFNLNTAEAATGWQTIDGKRYYFNTNTYIASTGYTIINGKHFYFNTD
                     GIMQIGVFKGPDGFEYFAPANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGL
                     RTIDGKKYYFNTNTAVAVTGWQTINGKKYYFNTNTYIASTGYTIISGKHFYFNTDGIM
                     QIGVFKGPDGFEYFAPANTDANNIEGQAIRYQNRFLYLHDNIYYFGNDSKAATGWATI
                     DGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIGVFKGPNGFEYFAPANTDANNID
                     GQAIRYQNRFLHLLGKIYYFGNNSKAVTGWQTINSKVYYFMPDTAMAAAGGLFEIDGV
                     IYFFGVDGVKAPGIYG"
BASE COUNT         1917 a          747 c          747 g         2015 t
ORIGIN      
        1 tattcaaaga aatttaaagt aatctatagt gttatgaaat attcaaaggg atttaaacta
       61 atctatagtc ttgtagaata ttcaaagaga tttaaactaa tttatgatgt tatgaaatat
      121 tcaaaggaat ttaaactaat ctatagtctt gtagaatatt caaagagatt taaagtaatc
      181 tatagtgtta tgaaatattc aaagagattt aaagcaatct ataatgctgt gaaatatatt
      241 caaggggatt taaagtaatc tatagtgtta tggaatattc aaagggattt gaagtaattt
      301 attgtgtttt gaaatattca aaaatatttt tggaatagtt tttataaatt taccagtatt
      361 aataatttta tgtttaacat ttagatgact taaaatcaac tatttgcact tttgtagtca
      421 tctaaaatca ttttataata tttttaaata atggaaagga tgtttgaaca tgtttgaatc
      481 aagatgtggt gtctgttgtg acagttgtac aaaaaaagaa caagtaaatt gcactggttg
      541 cccaactatg gaaaaaccat tttggggtgg agaatgtaag gttaaaactt gctgtgaaag
      601 taaggaactt aatcattgtg gtgaatgtga tactttccct tgtgatatgc ttttaaataa
      661 gggaaaagac caaggctttg accctatggt aaatattggg caatgtagaa aatggcttga
      721 agaaacagtt tctaattaat tgttttagaa tgctttgtta agtaatttat ttaatccttt
      781 gttaggtagt atgtttagtc atttattgat tggtatattt atacatttat tgatgggtat
      841 atttagccat acaaatagat ttacataaaa tttacgacta aaaaagagtt gagtattagt
      901 ttttattatt taaacggata ttcaactctt ttttatatct aacttttttg attttttaat
      961 ctttccaata ccaccacttt aatttttctg gttctggatt ttcagttgat gcaaaatata
     1021 ctgcacatct gtatacatat aactgacatc tgtctatgtg ctgtccacgc attaaacatt
     1081 ccttttcata catttgtgct gggtttgcat ctttaagtga tttaatagtt gtatatccta
     1141 acatgattaa agatttttct gttgctttcc ctacccctgg aattttttgt agttctccca
     1201 tacttcacct tctttctgat atattatttt tgtattatac ttagtaccag atattttttt
     1261 attatagtta atatttaatt ttttattata tcactttatt tatgctcttt catctatcta
     1321 tattttacca cctctaaagt actgaatcat ttaattacat cataatatag ttttatacaa
     1381 ataaaatact ttatgtttca tttaatatat aaaattcacc ttcaagaaaa ttatattata
     1441 atctgacatt tttacctcat ttttcaaaat atattgaatc ttcttgattt attttgtaaa
     1501 attatgctta ggggaaatat attttaggaa aatatgaata tataattttt agtcaactag
     1561 ttattttaag tttttaaatt ttaaaataaa atatatctaa taaaagggag attgtattat
     1621 gttttctaaa aaaaatgagg gtaacgaatt tagtaatgaa agaaaaggaa gctctaagaa
     1681 aataattaaa ttctttaaga gcacaaagga tattgctcta ctggcattta ttttggtgtg
     1741 ttttttggca atatatcctc accagcttgt tctgaagacc atgaggaggt catttctaat
     1801 caaacatcag ttatagattc tcaaaaaaca gaaatagaaa ctttaaatag caaattgtct
     1861 gatgctgaac catggttcaa aatgaaagac gacgaaaaga aagctattga agctgaaaat
     1921 caacgtaaag ctgaagaagc taaaaaggct gaagaacaac gtaaaaaaga agaagaagag
     1981 aagaaaggat atgatactgg tattacttat gaccaattag ctagaacacc tgatgattat
     2041 aagtacaaaa aggtaaaatt tgaaggtaag gttattcaag ttattgaaga tggtgatgag
     2101 gtgcaaataa gattagctgt gtctggaaat tatgataagg tcgtactatg tagttataaa
     2161 aaatcaataa ctccttcaag agtgttagag gatgattaca taactataag aggtataagt
     2221 gctggaacta taacttatga atcaactatg ggtggaaaaa taaccatacc agggatagct
     2281 gtagagaaaa ttaattaata ttgttttgta ttatagttaa tattttatat tatagtcaat
     2341 atgtttaaag atgtttttat aattgcaaat aaacagttac aaggctctaa attagttttt
     2401 gcttttagca tattatctat tttctatcaa ctattaatta tttagtatta atatttccat
     2461 atatgaattt tattataaaa tagtcaagaa taatagatta ttaaatgata gaaaaatttt
     2521 aactaaaagt catgtattac aataacacat gacttttaat taaatctcaa tatttattat
     2581 ataaaaataa tttctgagta tcacaggaat aattttttgt caaacatata ttttagccat
     2641 atatcccagg ggcttttact ccatcaacac caaagaaata tataacacca tcaatctcga
     2701 aaagtccacc agctgcagcc atagcagtat caggcataaa gtaatatact ttactattaa
     2761 tagtttgcca tccagtaact gcttttgagt tattaccaaa gtaatatatt tttccaagta
     2821 aatgtaggaa tctattttga taacgtatag cttgaccatc tatattgtta gcatccgtat
     2881 tagcaggtgc aaagtattca aatccattag gtcctttaaa cactcctatc tgaggtaaac
     2941 catttctaaa gtaaaaattt ttattatcaa tagttttata accattcgca cccatagctg
     3001 tattaggctc gaagtaatat ctattaccat caatagttgc ccaaccagta gccgcttttg
     3061 aatcattgcc aaagtaatat atattgtcat gtaaatatag gaatctattt tgataacgta
     3121 tagcttgacc ttctatgttg ttagcatccg tattagcagg tgcaaagtat tcaaatccat
     3181 caggtccttt aaacactcct atctgcataa taccatcagt attaaaataa aaatgtttac
     3241 cactaataat tgtataacca gttgaagcta tataagtgtt agtattaaag tagtattttt
     3301 taccattaat agtttgccat ccagtaactg caacagcagt gttagtatta aagtaatatt
     3361 ttttaccatc aatagttcgc aatccggtaa ctgcttttga gtcactacca aagtaatatt
     3421 ttttaccatt caaagttaag aatttatttt ggtaaagtat agcttgacct tctatgttat
     3481 tattatgagt attagcaggt gcaaagtatt caaatccatc aggtccttta aacactccta
     3541 tctgcataat accatcagta ttaaaataaa aatgtttacc attaataatc gtataaccag
     3601 ttgaagctat ataagtgtta gtattaaagt agtatctttt accatcaata gtttgccatc
     3661 cagtagctgc ttcagcagtg ttaagattaa agtaatattt ttcaccatca atagtttgcc
     3721 atccagtaac tgcaacagca gtgttaagat taaagtaata ttttttacta tcaatagttt
     3781 gccatccagt aactgctttt gagtcattat caaaataata ttttttgcca ttcaaagtta
     3841 agaatttatt ctggtaaact atagcctgac cttctatgtt attattatga gtattagcag
     3901 gtgcaaaata ctcaaatcca ttaggtcctt taaatactcc tgttaccatt ttagattcat
     3961 tattagcatc aaaatagaaa ttatttcttt caatagtaat atatccattt tgaagaattc
     4021 catcataact aaagtaatac ttgtcgttat ttatagtgca tagatgagtc gcagcaatag
     4081 cattattagg attaaagtaa tattttttac catcaatggt ttgccatcca gtaattgctt
     4141 ttgagtcact accaaagtaa tattttttac cattcaaagt taagaattta ttttggtaaa
     4201 gtatagcttg accttctatg ttattattat gagtattagc aggtgcaaag tattcaaatc
     4261 cattaggtac tttaaacact cctatctgca taataccatc agtattaaaa taaaaatatt
     4321 taccattaat aattgtataa ccagttgaag ctatagaagt gttagtatta aagtaatatt
     4381 ttttaccatc aatagtttgc catccagtag ctgcttcagc agtgttagta ttaaagtaat
     4441 actttttacc atcaatagtt tgccatccag tagctgcttc agcagtgtta gtattaaagt
     4501 aatatttttt actatcaata gtttgccatc cggtaactgc ttttgagtta ttatcaaagt
     4561 aatatttttt accattcaaa gttaagaatt tactttgata aactatagcc tgaccttcta
     4621 tgttattatt ataagtatta gcaggtgcga aatattcaaa tccattagag ccactaaaca
     4681 cacctatttt cactacacaa tcactatcaa aataaaagtg tttaccatca atagttttat
     4741 aaccattaaa ggcaatagcg gtatcagtat caaagtagta tctactacca ttaacagtct
     4801 gccaaccttt tgagatgata gcagtgtcag gattgaaata atacttatta ttgtcaatta
     4861 cttgcaatcc gactgcagca atagcattat taggattaaa gtaatatttc tcattgttaa
     4921 taatcctcca tccagtgact gcttttgagt cattatcaaa ataatatttt ttgccattca
     4981 aagttaagaa tttactttga taaactatag cctgaccttc tatgttatta ttctgagtat
     5041 tggcaggtgc aaaatactca aatccatcag gtcctttaaa tactcctaac tgcatcacac
     5101 cattattatt aaaataaaag tgtttaccat taataatttt ataactagtt gaagctgctc
     5161 cagtatttat atcaaaataa tattttttac cattgatagt ttgccatcca gttactaagt
     5221 tagattctat aggatcaaaa tagaataatg aattatttat attgattaat cctttaacta
     5281 atttactatc ttcatcatag taataagttt tattatctat tattttaaat cctaaatgat
     5341 ctctatctaa ttcattttca gaattaaatg atttaaaatt actcattata tatcctaatg
     5401 atagtttttt aatatcttta aaatct
//
LOCUS       DAEPLF010000221         5405 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEPLF010000221 DAEPLF010000000
VERSION     DAEPLF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.222"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            131..1327
                     /locus_tag="KR314_003140"
     CDS             131..1327
                     /locus_tag="KR314_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0329500.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(1439..2785)
                     /locus_tag="KR314_003141"
     CDS             complement(1439..2785)
                     /locus_tag="KR314_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH0329501.1"
                     /translation="MTMKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVV
                     EERQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKI
                     PLQFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALK
                     RVFPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDL
                     VPKIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKV
                     AKSNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAH
                     NKHQIAIVITENNGAINNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAY
                     EKLAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(2918..3670)
                     /locus_tag="KR314_003142"
     CDS             complement(2918..3670)
                     /locus_tag="KR314_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0329502.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(3829..>5405)
                     /locus_tag="KR314_003143"
     CDS             complement(3829..>5405)
                     /locus_tag="KR314_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0329503.1"
                     /translation="KQKMKEFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSDI
                     PFLENNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVLH
                     TSVNYMLGCRGILAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNIK
                     LPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFVF
                     GFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFSTE
                     ATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLEF
                     SFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSII
                     LGLVYALVYYNVFKVLILRFNLQTPGREEITDINFDLEPLSNKVEIAQSGETTKRALS
                     IIKYLGGKENIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIYG
                     PSVSQVRDEVSFAMSI"
BASE COUNT         1876 a          886 c          701 g         1942 t
ORIGIN      
        1 gctatattaa ttttttttga tgatttttac ccataagtat gttattatta tcttgtaaag
       61 atgtcttgta ttcaaacata caatttacat aatacaatat acgtatttaa attaaatagg
      121 gggtattaaa atgaattatt caaacagagt tagtgcaatg caagcctctc caatcagaaa
      181 acttgttcca tttgcacaag cagcaaaaga taaaggaatc aaagtatatc acttaaacat
      241 aggacaacca gacataaaaa ctcctaaagg attttttgat gctgttaaaa actttgatag
      301 cgaagtctta gaatatgcta catcagaagg tattccagaa ttattagaag cacttcaaaa
      361 ttactataaa acttacgata tgaattttga aaaagatgaa ttattagtaa ctaatggtgg
      421 tagtgaagct ttacttttca ctatgatggc agtttgtgac ccaggagata atctattagt
      481 tccagaacca ttctatacaa actacaatgg atttggtcaa tctgtaaatg tagaagttaa
      541 tgctgttaca acaaaggcag aaaacggttt ccaccttcca tctaaagaag aaatattgtc
      601 taaagtagat gataaaacta aggctataat tctttctaac ccaggaaatc caactggtgc
      661 aatttataca aaagaagaac ttaatatcct agctgaaata gctaaagaaa aagatttatg
      721 gataatagca gacgaagttt atagagaatt tgtttatgat gggcttgagt atacaagctg
      781 tggtaactta gagggtgtac aggatagagt tataataata gacagtgttt ctaagagata
      841 cagtgcttgt ggtgctagaa ttggttctat cgcttgcaaa aataaaggtc ttatcgctca
      901 aatacttaaa ttatgtcaag gaagactttg tgttcctaca ttagagcaaa taggagctgt
      961 tgagttatac aaaactcctg tttcttactt caaagaagtt aatgaagagt acaaaaaaag
     1021 aagagatgta ttatacaatg aattaatgaa ggttgaaggt gttatatgta aaaaaccaac
     1081 tggtgctttc tatatagttg ctaaattacc agttgaaaat gcagaagatt tcacaatttg
     1141 gatgttaaaa gaatttaaca aagataatga aacagttatg gtttgccctg ctgaaggatt
     1201 ctatgctact ccaggtttag gacgtgatga aataagatta gcttacatct taaatgaaaa
     1261 agaccttcat agagctgcta cattattaaa agaaggttta gagcaatatg tagctttaca
     1321 aaaataattt tttaaactta atttatataa atttaatgat ataaaatgtc cccttttcag
     1381 atattatttg aaaaggggac atttcttaat taatatatta attaagactt agacctgact
     1441 atttttcatt ttctttatat agtgttggcc agtaatcttt attcacttta attaaagcat
     1501 ctaaaacttg tcttgcctta gttggagaga ttatagttct atttaatgtt aaagataaaa
     1561 tagctttttc ataagattgt tcaaagtatg catcaactgc taacttttca taagctagtt
     1621 gaccttctaa cagtccctta taaaatgttg gtatattacc tacatgtaaa ggtctcggtc
     1681 cattctttgt aagaatcgca gctacttcta ccattgcatc atcaggtaag ttgttaatag
     1741 ccccattatt ttctgttata actatggcta tttggtgttt gttatgagct attgcttctg
     1801 caacttcaac tataaattcc ccatgagcat cattatgcac taaattagaa cctatagatg
     1861 ttcctttttc tataacagat ttacattctt caaatacacg cttttctctt ccattcataa
     1921 cttctcttgc tctagtacaa tttatatttg atttagctac ttttttatca ggatatagat
     1981 aatactgtac atatgtatta ggtaagtaat ctggtacatc ttccaacata tctttaacca
     2041 ttccataagt gtctaaccaa gatttatctc tttgttctgc atcaacaggt tggaatccag
     2101 agtttgctac taaatctttt atcttaggta ccaaatcttc accattttta tcatacaaat
     2161 gtgtaaacca tccaaagtga tttaatccaa agtatacagg ctcaaatgtt cttgaatcca
     2221 tgtctaacaa tcttccataa gaccttaaca agttaactgg ttggtcacac atattaagta
     2281 atcttttatc atctggaaat actcttttta atgcttctgc aacaatagca gcaggatttg
     2341 tataatttaa tatccatgct ttaggagagt attttcttac agtcttaacc atttctatca
     2401 tatcacctat agaccttaac ccataagcaa atccaccagc tccacaagtt tcttgaccaa
     2461 ccagtccaaa ttgtagagga atcttttcat ctaattctcg catcttaaga ccaccagttc
     2521 tcatttgaac aaatacaaaa tctacatcta taaatgcttc atctgggttt gtagtatatt
     2581 caagtgttgc tttaggatat tcttctttaa ataatatttt tccaaattcc cctattaatc
     2641 tttgacgttc ttctactaca tctaacatag ttactttatt tagaggaaat ctatcactca
     2701 ttttacataa agactttaaa agtcctggag tccaagtact gcctcctcca actatagcta
     2761 tattgtatct ttttttcatt gtcatattaa tcacccctgt tttttattaa ctaacttgta
     2821 agatgattat aatatataaa gttttctaca tgaatacttt taggtaatca ttaaaaatag
     2881 ttcaatttta gtattttttt caaaattgta atttttttca tctattgtat ttttcaatgt
     2941 atttatgaaa catatattcc ataagagaaa gtgctggcac tctagacgta tagttaacac
     3001 tattaaaatt atattgtgga gaagatacaa ataattttat atcagctata tttgataatg
     3061 aattgacact tatatttgtc agagaaatta ctttaacttg atttgttttt gctatattac
     3121 atgccttaat aacttgagca gtctctccag acatagatac agcaattaca aggtcatctt
     3181 tagtaatatt tgatgcagcc aaatacatga gagttgaatc atcatagtat tttacattta
     3241 cacctatagc tgaaaaacgt tgagacattt cagtacatac taatctagaa gacccaacac
     3301 caaaaaaata tatatgctta gaattatgta tttcatttaa acacatatct atattctcta
     3361 catttattaa attttgtgta tccataacat cagtaaaaat atcactttga cttttagtaa
     3421 actccttatt tctttcattt ctatttgaat atgaaaaaac taaaaagttt ttaaattcac
     3481 tatatccaga aaagccgagc ttctgacaaa accttactat gtatgcagtc gaattaaaag
     3541 tttcatttgc tatatcttta atcttttttt gatttatttt ttcagaattt tttagaaaat
     3601 agtttaaaag agatatttca tttgtagata attttttttg atttatatct aatttgtcta
     3661 ttaggttcat ttatacctcc ataataatga tttgtagtta aacaactttc tcatatttat
     3721 tatataaata tacactatac aacatttatc ttatgtttat tatactacaa aaaagacaat
     3781 acaaattata aattagtatt gccttttaat atttacatta gatagacatt aaatagacat
     3841 tgcaaaagaa acttcgtctc ttacttgaga tacagatggc ccataaatta tttggatatc
     3901 tttatcagat acttttataa cgcccattgc acctgtatat tttttaaaag cattttcatc
     3961 tactttactc atatctttaa ctactactct taagcgagtt atacagtttg ttatatcttc
     4021 tatattctcc ttaccaccta aatatttaat tatggataat gctctcttag tagtttctcc
     4081 actttgagct atttctactt tatttgataa aggttctaaa tcaaaattta tatctgttat
     4141 ttcttctcgt cctggagttt gtaagttaaa tctaagtatc aaaaccttaa atacattata
     4201 ataaaccagt gcatacacta aaccaagtat tatagaataa atccactttg tatttggttg
     4261 cataactcca aatactacta aatctattat gccaccttga gtatttccta ttgctactcc
     4321 aaatatatgc atcatcataa atgatatacc tgataaaaat gcatgtataa caaataatat
     4381 tggtgataca aataagaatg aaaattccaa tggttccgta attcctgtta caaatgttgc
     4441 taaagcacca gctagtaata tacctttaac cttcgaccta ttttcaggct ttgcagtttt
     4501 atacatggca tatgctgcag caggaagacc aaataccatt ataggtattt tcccttgagc
     4561 taaaaatcta gttgcttctg ttgaaaaact gattgtttca gggtcactta gttgagctaa
     4621 aaatatgttc aatgctccaa atacttcttg tcctcctaca actaaagaac ctcctacttc
     4681 agtaaatcta aacatagcat ttaatatatg atgtaatcca aatggtatta aaagcctttc
     4741 aagaaatcca aatacaaagg ttcctaaata tcctaaacta gctataccaa tacctacact
     4801 ctgaataatt gtatttacat atggccatat atatggtatt attatcccca aaatagagaa
     4861 tactactgtt gttattattg gaacaaacct agttccacca aagaacccta aataatctgg
     4921 cagttttata ttatagtatt tattatgtaa tgatgctgtt attataccaa ctacaattcc
     4981 accaaatact cctaactcta atgtttgtat gcctaaagtt acagcttgac ccatttctct
     5041 catcatgtca ggttcagcaa gaatacctct acaccctaac atataattta cactagtgtg
     5101 taatactaaa taaccaacaa aaccagaaaa tgcagaaact gctttttctt ctttagcaag
     5161 accaacagat attgatatag caaagaatat tgctaagttt ccaaaaagta ctcctgttat
     5221 agcttttgta aaacccaata ttccttgaat aaaattattt tctaaaaaag gtatatcact
     5281 aactatatta ggatttgaaa atgctgaact tattcctaaa aatatacctg cagcagatat
     5341 taatgctatt ggcaataaaa atgattttcc taactgtaaa aaaaactctt tcattttttg
     5401 tttca
//
LOCUS       DAEPLF010000222         5315 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEPLF010000222 DAEPLF010000000
VERSION     DAEPLF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.223"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..836
                     /locus_tag="KR314_003144"
     CDS             <1..836
                     /locus_tag="KR314_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HBH0329504.1"
                     /translation="KAIFIDEAESFLEYEIDFIREFLYKTKFIFNVCSCNSLNISNRL
                     NIFKKLYNGIEFDDKIILSKNYRQAKEIVDFTNKFSNNSNSYINELRPNTEFSTFFYT
                     KALRGGNKSVDIIKVSDLDDQISSVIWEIEYLISKKGLDYSEVAIVYPYNKKKLKSGK
                     TIYFQYMLKKALEEAKIPYICAEDNLTNISKKVGTTIANIYAIKNLEYKAVIVCELEM
                     LYNQTINDIEQDYQVNDFVGDLNKVYLAMSRATDYLSIVTTFNEEASDIIRLITESKD
                     I"
     gene            complement(918..1448)
                     /gene="lepB"
                     /locus_tag="KR314_003145"
     CDS             complement(918..1448)
                     /gene="lepB"
                     /locus_tag="KR314_003145"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBH0329505.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSVVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEILG
                     KVMVRLLPLDNIGKVD"
     gene            complement(1488..2312)
                     /locus_tag="KR314_003146"
     CDS             complement(1488..2312)
                     /locus_tag="KR314_003146"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBH0329506.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILINEFIKLNF
                     SNTFINTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRVFFNSTVVAIALIVYGIIMIGLEKRNKRPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            2569..3477
                     /locus_tag="KR314_003147"
     CDS             2569..3477
                     /locus_tag="KR314_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0329507.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            3621..5066
                     /locus_tag="KR314_003148"
     CDS             3621..5066
                     /locus_tag="KR314_003148"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBH0329508.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINKRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
BASE COUNT         2148 a          678 c          798 g         1691 t
ORIGIN      
        1 tcaaggctat ttttattgat gaagcagaaa gctttttaga gtatgaaata gattttataa
       61 gggagttttt atataaaaca aaattcatat ttaatgtgtg ttcttgtaac tccttgaata
      121 tctctaatag attgaatata tttaaaaaat tatataatgg tatagaattt gatgataaga
      181 ttatcttaag taaaaattat agacaagcta aagagattgt agattttaca aataaatttt
      241 caaataattc taactcatat atcaacgaat taagaccaaa tacagaattt agtactttct
      301 tttatactaa agcacttaga ggtggaaata agtctgttga tataataaaa gtaagtgacc
      361 ttgatgacca aataagttct gtaatctggg aaattgagta tttaataagt aaaaaaggtc
      421 ttgattattc tgaagttgct atcgtttatc cttataataa gaagaaatta aaaagtggaa
      481 aaaccattta ctttcaatat atgttaaaaa aagcattgga agaggcaaaa attccttata
      541 tatgtgcaga agataatttg acaaatataa gtaagaaggt aggaactaca atagcaaata
      601 tatatgcaat aaagaattta gagtataaag cggtaatagt atgtgaatta gagatgttat
      661 acaatcaaac tataaatgat attgaacaag attatcaagt aaatgacttt gtaggagatt
      721 taaataaggt ttatttagct atgagtagag caacagacta cctaagtata gtaacaactt
      781 ttaatgaaga agcaagtgat ataataagac ttattacaga atcaaaagat atttagtaaa
      841 aaaatatttg taatttaaga aaataaaaag agactgagat ttgtaaaata aactttacca
      901 attcagtctc ttatttttta gtccactttc ccaatattat caagaggtaa tagtctcacc
      961 ataaccttac caagaatttc atcttcatca accataccta catcagggaa tctactatca
     1021 ttactatttt ctctattatc tcccattgca aatagtttac cttttggaac aacagtatct
     1081 atatctccag aagtatagtt attgtgtata tatggttcat ttaataattt tccatttaca
     1141 tacactttag aatttgatat ttttattctg tcaccttcag tagctataac tctttttatt
     1201 aagtcttttt tctttcctgt ttctccatca actaaattgg ttttaaaaac tacaatatcg
     1261 cctttttcag gtttaccaac cttgtatgat atcctattta aaatcagata atctttatca
     1321 tctaaagtag gatacattga ttctccactt acaacagaag gtcttataaa ttgtagaatt
     1381 acaaatgcaa gtacaatagc catagctatt gacttaatcc aatcaaatat ttctttttta
     1441 acactcataa cttttctcct tatcatttgt tattaaatta gaatacatta taatagaaag
     1501 aaataagcaa gaaccactat acccaagact attctataat aaccaaatac tttgaagtca
     1561 tgttttttga tatagtccat aaaaaattta ataactacaa ttgaaacaac aaatgctact
     1621 acagaaccaa cacctaaaat aagccattca aatccagtaa atccaaatcc agcttttaga
     1681 agttttaatg cactagctcc aagcattgta ggaatggcta agaagaatga aaactctgct
     1741 gctacatatc tagaagtacc aagtaatact gcacctatta tagtagaacc agacctagat
     1801 gtacctggta tgagagctag acactggaat aatcctatac aaagagctaa tttatatgta
     1861 acttgagaaa aatctttata cttaggtctt ttatttctct tttcaagccc tatcattatt
     1921 ataccataga caataagagc aatagcaact actgttgaat taaagaatac tctgtctatg
     1981 tcatcttcaa acagtattcc aataactcca gatggtataa ctgcaattat aaccttaagc
     2041 cataaacgaa ctgtttcttt tttctgttta atattctttg cactatcaaa aggatttaac
     2101 tttctaaaaa atataacaag tacagctagt attgaaccaa attgaattac aactaaaaat
     2161 gtatttataa atgtatttga aaaattcaat tttataaact cattaattaa tatcatatgc
     2221 cctgtactac ttactggtag ccattctgtt attccctgaa ctatgcccaa aatgactgcc
     2281 ttaaaaattt caagcaaact aaatgtttcc aaatcaaaat acctccaata caaatttata
     2341 gtagcatatt ttattcatag attaataaaa aaatgccacc ttttactaac ttataatacc
     2401 atagtactgt gtagtgcaca atggaagatt gatattttca taaaaccttc ataatataac
     2461 aaaataaact taaaagttat ctattgaata aggggtatat ttgaggggga aatatatggt
     2521 atagtaaaag tatagctatt ttattttatg aaaaggaaag gagttattat gtatagtaca
     2581 aaaatactag atgattatga gtatgaaact tttaatgttt tttctacaaa attaaatcca
     2641 aacacgaaac acgattattt atcaaaggtt atactgttta aagaattttt aaaagggaaa
     2701 gaattgattt atgctacaaa agaagattgt aaaaattttg tagactatat acagactaaa
     2761 tatgcgaaat caacttgtga aaagatatat agttatttac atagttttta taacttctta
     2821 aaaaaagaag gatacataga tataaatcca tttagatatg tagaaaaacc cacagtaaca
     2881 agaataaaaa caaaagatga tgttctaagt atacaagaaa taaataaact tataggtatc
     2941 ctaccaaaat taaatataag agatagagta ataatagtct gtctagttac tactgggtgt
     3001 ttattaaatg aattagttag cttaaagtgg aaagacctta tggtagatga aaacgataac
     3061 tcatatgtaa ggttaggtaa gggaagaaaa gaaagagtag ttaaattaca tccatacttt
     3121 tttaaacttt tagaagatta caggaattat tctgggcttc cagaggtaat aatacctagt
     3181 gatgatttta tttttacaac tcaaaagagt aattctataa cagatagaaa tgtcagactt
     3241 atagtcaaaa aagcattaga tttagctgga ctttctcaat attcagcaag agattttaga
     3301 cattcatttg cagcaataag tttaaggctt ggggcagatg aaagtgatgt aaaaaatcag
     3361 cttggatgga gtgataaata ctatgcaatt agatataagt atgtattaaa ttttgttgat
     3421 agtgaaatag tggattactt aattgaaaaa gataatttgg atataaataa aaaatagtaa
     3481 taaataaaaa atactatata tagatgtgaa caaaaaataa aaaagtcttg ttatagttcg
     3541 taaaattata tataattatt tatgtactaa tgtgtacaca gtaatcacac gacaaaagat
     3601 ttaaggaagg tgctaatata atgatagata ataaatatac tacttactca aacccactta
     3661 cagacagata ttgtagcaag gacatgagtt atatattttc tccacaattt aaattctcta
     3721 catggagaag gttatgggtt gcacttgcag aaggtgaaca tgagttagga atcaatataa
     3781 ctgaagaaca attagatgag ctaagagcta atatagataa tataaatttt gatgaagcta
     3841 gaaaaataga aaaagaagta agacatgatg taatgtctca cgtaaaagct tatggtttac
     3901 aatgtgaaaa agccaaagga ataatacatt taggagcaac atctgcatat gttggagata
     3961 atacagatgt tatacaaatg aatgaagctt taaaacttct aagagtaaag cttgtaaatt
     4021 taataaacaa tttaaaagaa tttgcactta agtacaaaga tatgccagct cttggattta
     4081 cacacttcca agctgctcaa cttacaacag taggaaaaag agcttgttta tgggctcaag
     4141 atttaattct tgatttggaa gatttaaact atagaataga taacatgaag ttaagaggag
     4201 ttaaaggtac tactggtact caagcaagtt ttttaagctt atttgaagga gaccacgaaa
     4261 aagtaaaaga gttagataag aaaatttgta aaaagatggg ataccattct tcctatgcag
     4321 taagtggaca gacatatact agaaagttag attatcaagt aataagcata ctatcaggga
     4381 tagctcaaag tatgcataag atgactaacg atataagact tttacaaagt ttaaaagaat
     4441 tagaagagcc atttgaaaaa aatcaaatag gttcatcagc aatggcttat aagagaaacc
     4501 caatgagaag tgagagaata gcatctttat ctaagtatat aatctctgaa tctataagcc
     4561 ctgcaatggt acaagccact caatggttag agagaacatt agatgactca gccaataaga
     4621 gattggcaat acctcaagca tttatggcag cagatgctat acttgaaata gggataaatg
     4681 taactgatgg tttagtagta tatgaaaata tgataaataa acgtgtaaat gaagaacttc
     4741 cttttatggc tacagaaaca atacttatgg aagctgtaaa aagaggtgga gatagacagg
     4801 aattacatga aataataaga gaatattcta tgaaagctgc ctatagagta aagcacgaag
     4861 gtaaggataa taacttaatt gaacttataa tgaatgatga ttcatttaaa atgagcaagg
     4921 aagaaatact atctataatg gacccaaaaa actttatagg tagagctcca gaacaagttg
     4981 tagaatttgt aaatgaagtt gtagaacctg ctataaaaga ttataaggaa gacttaggaa
     5041 aagtagatgt ggacctaaga gtatagaatt gtacagataa aatataatta aaaacggcta
     5101 tcttatttat aaataagata gccattttaa tgggctagcg tcttctcttc agcgctgtga
     5161 agagttcttc tttacttact ataccatgaa tttaataaaa tagctatata aataatattt
     5221 aatagaaata atataaataa aaataaaaaa atgataagat tactaattaa atttaaacat
     5281 tcagcaaaaa ctattgtact tgcaatattt ttaat
//
LOCUS       DAEPLF010000223         5306 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEPLF010000223 DAEPLF010000000
VERSION     DAEPLF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.224"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            164..1690
                     /locus_tag="KR314_003149"
     CDS             164..1690
                     /locus_tag="KR314_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0329509.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NNGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1718..2275
                     /locus_tag="KR314_003150"
     CDS             1718..2275
                     /locus_tag="KR314_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329510.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            2797..3840
                     /locus_tag="KR314_003151"
     CDS             2797..3840
                     /locus_tag="KR314_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329511.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKG
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            3951..5093
                     /locus_tag="KR314_003152"
     CDS             3951..5093
                     /locus_tag="KR314_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0329512.1"
                     /translation="MSEEKNPKENTLDEPKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2249 a          429 c          935 g         1693 t
ORIGIN      
        1 tagaaattat aaatatatat aagtttagtc tggtgtataa aacaagaaaa ctaagtgatt
       61 tttcatttag ctttttatta agcttatttt ttatactatt tataataaat aaactccatg
      121 atgggaaaac tattactatt taatatgtaa aggagtattt taaatgaatg aatcatattg
      181 gtttttaaac tcaagtccaa aagaatataa caaattaggt gaaaatataa aaacagattg
      241 tcttatagta ggtggtggaa taactggatt gacaactgca tatttattag caaaagaagg
      301 taaaaaagta gtgcttgttg aggctgataa aataggatat ggaacttctg gaaggaatac
      361 tggtaaggta acttgtcagc atgatatatt ttactctaag atagagaaaa aatatggatt
      421 agataaagca aaatcatatt ataatgcaaa taatgaagct ttaaatttag ttgaacaaat
      481 aatagaagaa aacaatataa aatgtgattt taaaagggaa acttcttttg tatttacaga
      541 aaaagaggat actataaaga atattaaaga tgaatatcgt acatgtaaaa aaataggaat
      601 aaattgtgag tatcatgaaa ctatagaaaa tattccatta gatataaagg gggctattag
      661 ttttactaat caaggtcaat ttaacccaaa gaagtatatt gatggattgg caaaagcagc
      721 agtaaattta gggctaaaaa tatatgaaaa tacaccagta gtagatcttg aaaaaggaaa
      781 aatatgtagg gttaaaacaa gagaagataa tataatagaa gctgagaatg ttataatttc
      841 atctcatagt ccatggtatg atggattgaa tttttatttt gcaaaagaat atgcagagcg
      901 tgcttattta atggttgcag ttttggaaaa taaattagca gatggtatgt ttataagtat
      961 agatgaccca tcaataactt ttagacaata taataatgga agtgaaaatt tactgatttt
     1021 tggaggagga gaccataaag ttggccaggg aggaactgag aaagaaatat ttgatgattt
     1081 agagcattat ggtaaagaag tctttaaggt aaaagatttt aaggggaaat ggtctgctca
     1141 agataatatg agttttgaca atgttcctta tattggttat atcaacaaaa gagaagacaa
     1201 tatttatgta gctacaggat tctctaaatg gggaattact aatggaactg ctgctggaat
     1261 aataataaaa gatttgataa taaataataa ttctgattat aaagatactt ttaatccatc
     1321 cagattaggt agctactttt caaaagattt tataaaggaa aatgcaaacg tagcaataaa
     1381 ttatgtaagt ggaaaattaa aaataggtag tggagatatg cctaaaaata atggagaagg
     1441 aaagattgtt aatattgatg gaaaaagata tggtgtatat aaggatgata atggagattt
     1501 ttatattgta gacacgacat gtactcattt aggatgtgaa cttaatttta acagtgaaga
     1561 aaaaacatgg gactgtccat gtcatggttc tagatttgac tataagggaa atatacttga
     1621 aggaccagct cttaagccat taaaacttta tggacatggt gataatgatg ttaatcctaa
     1681 gttattataa aattattttt aggaaggtaa agtgtatatg aataaacttt taaagccaat
     1741 aactatacta ttgtatatag tttatatttt atctgccgta ttttttgtag caaacaaaga
     1801 atatggaagt ctaggtctag tagcaattag tttagttggt actttcattt tgagttttat
     1861 aaataagcaa actgaaagat tattgagtaa agaattatat ataatgcttg ctttatttat
     1921 aatgttttca tcattgttag gtacttgttt taatttttat gatataaatt attatgatga
     1981 ttttttgcat ttatggtcag gtataatatc ttgtattgtg gctttttcta taattaaata
     2041 cttttataca caaagagaga taagtaaaat gagtaaagtg tttttagtta tatttttatt
     2101 tatgtttagt atgggagtag cgagtttatg ggaaataggt gaatttttaa tggatacttt
     2161 tgttgggact actacacagg ccggggggtt agaagatact atgattgata tggtagatgc
     2221 tttaataggc acaattataa ctattccttt tatagtaaaa aaatctaata tatagtttta
     2281 aattttttat attaattata atggatttaa ttaatcatgt tgagtttcaa ggtctatatg
     2341 attaattaaa tccatttttg ttatttataa ttattattga atgtattttt acatataaga
     2401 tataaggtga tagatagaaa ttgttggatt atgtaaataa ttaatatata ataccattat
     2461 gggcaataaa tacaaatcaa atgataatat agtctattta tgtaaatgtt atattgtatc
     2521 ctaagtataa tagtaagttt tttgatagtg gtgtagaaag taaaaatcta ctagcctaaa
     2581 ttgatgtatg gattagttta ggaacagtaa ttgcatatct taagtttgat gtcatgttgg
     2641 gctactagaa ataggtcatc tttttcactt gaatatcttt tctttaaaca tgaggagtat
     2701 catatcaaaa acatatgaaa attaagtgat ttataagaac agtaatattt tatatgatag
     2761 tatagaatag taataaagta ttaggaggaa cattgagtgt taaaaaagtg gtttggtatt
     2821 gtgaaaaaaa gacaaaaaag tgagtcagta aaagaagaag gtgaagtaat attaaaaaac
     2881 gaaaaaatat tatctgaaga aaagttgata gatgaagaag gagttgtagt taatattgat
     2941 aatgaaatat taactaaagt agaagtagta aatgatgata atgaaataga agaaaaaata
     3001 atagaagaag attggttaat tagtgaaaat accataaaat tagatgataa agaagcaatt
     3061 attaatgata aaaacataga attatgtaaa aaagaagttc aagttgaaag tgaaaaaata
     3121 ggtttaaata agtttgaagg acttgaccaa aatgaaaatt ataatctaga aaaaaatgtt
     3181 attgaagaaa aaaaagtaag tgaatgtttg acagaagaag atttagagta cataaaagaa
     3241 attaaaataa aaagaggaaa aagtataaaa gctataaact tgtatactaa agaagagtgg
     3301 gtttttgaca ctcatataca gtgtagtaaa aaactcaaag ttccattagg atacataaga
     3361 gaaaatttaa aatatggata tatggattac tttggagatg caataaatta tttaagtgaa
     3421 gtattaaata tagatgaata ctgcaaaggt gaatggagct atctcgataa tagcaaatct
     3481 ccatctgaga tatttaatat tctaaacaat aaaatattta gtataaggct ttctaatgaa
     3541 aaaagaaatg aaattttgac aaatgataaa attgaagcat taaaaatgaa ttatagattt
     3601 gaatgtattg atgaagaata tgatgaatat tttaaaaaat ataagtctat aatcaaaagg
     3661 ggtggaaaga aaaaagttga attagtaaat aaaaaaggtg acattttaga aatatttaag
     3721 tctttagaag aatgtgccat ttatttgcaa aaggaaaaga atgaagttat acaaatgtta
     3781 aaatatggag atacaaaagt aggaagaaac tttataaggt atagtttgag aagtatttaa
     3841 tttcatttta aattcataag agtttcattt ctgattcaca catttaatat tattttaatg
     3901 taaaataata aaggtgaagt attgcacaat taaaattaag gagggtatta atgtctgaag
     3961 aaaaaaatcc aaaagaaaat acattagatg aacctaaaaa ttcagataat gtaaattcta
     4021 aaaaagatga tgtgtcagat gaaaaaaata ctgataacaa agttaaaaaa agggaaataa
     4081 agctaaacaa caaaacactt aaagctattg tagcaattgt tgcagtagga gctatatgtt
     4141 taggtggagg ctatgttttt ggtaaagaag ctggaagaaa attgcctgct acttcaaaac
     4201 actataatag cagtgaggta gtagctacta ttggggataa aaaaataaca ggtgaaatgt
     4261 taagaaagag aatggagcca ttattttatt taaatgcaaa aactactatg actgatgacg
     4321 aaatacgttc atatgaatca aatatgattg aaactatgat tacatctgat atattatcct
     4381 ctgaagcagc aaaagagaaa ataagtataa ctaatgacca actagatgct cagtataata
     4441 atttaatgag tagtatagaa tcagcaatgg gaatgactaa agaagagttt ttaaaacagt
     4501 ttgatttgac tgaaaaatat ataaaagatg atatgaaaaa agagctagta gcatcaaaat
     4561 atttaaatga aaaatcaaaa gttactgata aagaagcaga gaactattac aataaaaaca
     4621 agagcaatta tttacaagtt agagcttcac atattttaat aaaaactgtt gatgataaag
     4681 gtaagcaagt tagtagtagc aaaaaagctg aacttaagaa agaagctgaa gaaatactta
     4741 aaaaagccca agctggggaa gattttgcta ctctagctaa aaaatattct gaagatagta
     4801 gtgcagaatc aggtggagat ttaggtttct ttggtaaagg tcagatggtg gaatcttttg
     4861 aaaaagcagc ctttgcattg aagaagggag aggtatctaa caaacttgta gaaagtgatt
     4921 atggatatca tataattaag aaaactgatg aaaaatatca accatttgaa gaggtaaaat
     4981 cagatttagt aagcagttta acatcagaaa aacaaaacct acttatacaa aatttaaaag
     5041 aaaaatataa tgttaaaatt acagataaat atcaaatgaa agctaaagaa tagataaagt
     5101 caatttatta aaaaaaagaa aaattaactg tatggataaa agctattttt actctcaata
     5161 tagagagtct aaagtagctt ttattatttt gtaataaaat ctatttatat atatgaagtg
     5221 ataaataaat aacatgcaaa aaaatattaa aaagaaatat ttttaatttg aattttttta
     5281 atgaaaattt acaaaaatga aaaaat
//
LOCUS       DAEPLF010000224         5283 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEPLF010000224 DAEPLF010000000
VERSION     DAEPLF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.225"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            206..739
                     /gene="pyrR"
                     /locus_tag="KR314_003153"
     CDS             206..739
                     /gene="pyrR"
                     /locus_tag="KR314_003153"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBH0329513.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            1023..2234
                     /locus_tag="KR314_003154"
     CDS             1023..2234
                     /locus_tag="KR314_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBH0329514.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLLLRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            2533..4185
                     /locus_tag="KR314_003155"
     CDS             2533..4185
                     /locus_tag="KR314_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0329515.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEYLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGLLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLLEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGCFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYIISRNKKTPSRDKQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQITKLKNSF"
     gene            complement(4400..>5283)
                     /locus_tag="KR314_003156"
     CDS             complement(4400..>5283)
                     /locus_tag="KR314_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBH0329516.1"
                     /translation="DIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELSESENADIY
                     KIKGELITSYIYMVEKGMESIEIANFYDENCNDVTIELNKNLTPSENAQKYFKKYNKM
                     KHAKVEISHQISLNKEEIDYLENIVLSIENCENLAELQDIKEELAKVGYIKTQKKNSK
                     KDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIPGSHVIIKC
                     AGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMVIYETNSTI
                     YVTPEEETVAKLKVKSE"
BASE COUNT         2006 a          552 c          914 g         1811 t
ORIGIN      
        1 aaattatctt taaacgatac agagatatcg taagacaatt gctaaattac actgtatatt
       61 ttttatataa gagcttcttg tcttgtgatg agaagttttt tgttgtttaa attgaaattt
      121 taaataaaaa taaatttata aaaaattaaa agaaaggagt agatttgtca aatagatata
      181 ttatatcttt gacaataatt atgatatggt agaaaaagct caattaatgg atgaaaaggc
      241 aatagcaaga gcaattacta gaataagtca tgaaataatt gaaagaaata aaggtgtaga
      301 aaatctagtt ttagttggaa taaaaactag aggtgtgcca attgcaaata gaatttctaa
      361 gaaaatagaa caaatagaag gaacaaaagt agatacagga gatattgata taactttata
      421 tagagatgat ttggaaaaaa ttcatgttga acctgtagtt aaaggaactt atttagattt
      481 taatgtaaat gataagacag tagttttggt tgatgatgtt ttatacactg gaagaactgt
      541 gagagcttca ctagatgcga ttatagatat aggaagacct aaatcaatac aattagcagt
      601 tctagtggac agagggcata gagagttgcc tataagagca gattatgtag gaaaaaatgt
      661 acctacttct agacatgaaa ttatatctgt cagtttatta gaaattgatg gagaagattc
      721 agttacaatc aaagaataat tagttttaag tattatataa aagcttagca ttgaatagtc
      781 agggaggagg ataaagctta catagattgc acaaaataaa tattataatt ttatggatgt
      841 gagtcttaat gtagttttta ttctccttta tttgagtttt tgatgtcgta aaatgtaaaa
      901 taatgataaa ttatataata agtatataga ggttaaaaaa tagaatctag ggaatgataa
      961 ttaattaaaa gatattaatt aaacagagga tttatttata tataataatt ttggaggttt
     1021 ttatgaaaaa aacaataatg tctttacaac atctattagc catgtttgga gcgacagtat
     1081 tagttcccat tttgacaggt tttaatccgt cagttgcaat tttctgtgct ggtgttggta
     1141 cattgatatt tcatttttgt acggaaggaa aagtacctgc ctttttaggt tcaagttttg
     1201 cgtttatacc agttatacta gcagctaaag aggcgtatgg tggagattta gcatatgctc
     1261 aaggtggtat aatagtagca ggattaattt atattataat gtcgattata gtaaaaatag
     1321 ttggtgtaaa taaaattaaa ttatattttc ctgcacaagt tacaggagct atgatagtag
     1381 ttataggatt aaatttattg ccaacagctt ttagtatggc ttcagctaat tttgtaatag
     1441 catttataac tttagcaata gcaattttaa ccaataagtt tggtagagga tttataaaac
     1501 aattaggaat attaatagcc gttttttctg ggtatataat ctgtttactg ttaaggttag
     1561 tagatataac tactattaca gaagcaagtt tatttgcaat accgaacttt acagttccta
     1621 agttcagttt aggagcaatt gtgattatat ctccagttgt attagctgta tttatggaac
     1681 atataggaga tatgactact aatggtgctg tagtaggtaa aaactttata gaaaatccag
     1741 gattaaatag aacattactt ggggatggat ttgcgactat agttgcaggg tgtttaggag
     1801 gaccagccaa tacaacttat ggagaaaata cagcagtact tgctataact aaaaactatg
     1861 acccttcaat acttagacgt acagcaatat ttgctatatt gcttgcatgt gtaggaaagt
     1921 ttggtggatt tcttcaaagt ataccaggtt cagttatggg tggaattagt ataatgctat
     1981 tttcaatgat aacttatgta ggattaaaaa ctataaggga cagttcttgt gtagaaagta
     2041 agataaatat attaataata gcagttatat tattaatagg tcttggaaca acatatttat
     2101 ctaataaagg catttctatt ggaatcccta taacaagtac agtaaagata actggtctta
     2161 gtttagctgc aattgttgga attgtattaa acagaatttt aaataatcag gattttaaag
     2221 ttgaagaaga ataaatttaa agaataaatt gtatattgaa taactaaaat tagttaattt
     2281 tctatagaca tttgtaatta atgttgatat aatgattaaa aataaatgaa agaaagggca
     2341 aggtaactag gacaatgaga atataatcta ttttttataa tttaaagtat tgtaattttt
     2401 gaaaagccta ttttgatggc tttatttgca atgcttaaat taaggagagt agattatagt
     2461 ctatacgttt atttgttatg cctatttata gaatctctcc ttatatttaa gtgtgttaag
     2521 gagggatttt ttatgttgtt agtaaaagta gagaatttaa agaaatatta tgcagataaa
     2581 ttaattttgg atatagataa acttgaaata ttagaaaatg acaaaatagg tctagttggt
     2641 tcaaatggtc aaggaaaaac aacattatta aaagcaatat tgggtgaaat agaaattgat
     2701 gaaggatata cttaccttac agaaagctat tcttatataa gtcagagtga aaataatatt
     2761 gaaacatgta gtcatagtaa ggagaagagt cttttaaatg ctccagataa atttgaagaa
     2821 tacttatcag gaggggaaaa ggttaagtta aaaatagcag atgcgctgag taataagaaa
     2881 aatatcataa tagctgatga gccaacttca aacttagata aaaaaagcat tgggcttttg
     2941 gaagatatgt ttaaaaggca tgagggagca ttattattaa tatctcatga tagacgtttt
     3001 ctggatgagt tatgtacaac tatattagaa ctagaagatg gaaaactaaa agcttacaaa
     3061 ggcaattaca ctgattattt aatgcaaaaa gatgaagaag taaaaagagc tgattttgag
     3121 tatcaagaat atgttaaaga gaaaaaaagg cttgaaaaag ctcttttata taaaaaagct
     3181 ttaagtgatg gcataagaaa gacaccaaaa agaatgggta attcagaagc taggttgcac
     3241 aaaatgggtg gtcagactaa taagaaaaag ttggactcaa atgtgaaggc tataaaaagc
     3301 agaattgata aacttgaggt aaagaataaa cctaaagttt ctaaagaaat gaacattaag
     3361 attcaagatg gtatggagat aattagcaaa aatctattag aggtaaaaga tatgactcta
     3421 aagttagaaa ataagctctt gttagataat gtttccttta agataaaaag aggtaaaaag
     3481 atagcattat taggtgataa tgggtgtgga aaaagtactc tgataaaaga aatattggct
     3541 gataaaaatg acaatataaa aataaataac aaggtaaaag taggttgttt tgaccaaaat
     3601 caaagtttat tagatgagga aaagagtgtt ctatacaata ctaaagttaa tagttcattt
     3661 gatgaatctt ttataaggat aaacttaagc ctatttgggt ttaaaggtga tgatgtctac
     3721 aaaaaagtaa aagtacttag tggtggagaa aaggtaaaaa tagcactatg taaaataata
     3781 ttggaagata ataatttttt agtatttgat gaaccaacaa attacttaga tataaaatct
     3841 atggaagcac tggaaaaggc actgataaat actgataaaa ctatgcttat agtgtctcat
     3901 gatagagtat ttgtatcaca tatttgtaac tatattatag agataaaaga tgctaaaata
     3961 agggagtttg attgtaatta tgacgaatat ataattagca gaaataaaaa aactcctagt
     4021 agagacaagc aaatcaaaaa agagaatctt ctagtattag aaaatagact tacaactgtt
     4081 atatctatgt tatctataga aaaagataat ctaaaaaaag aattgtatga atcagaatat
     4141 aatgaattac taaaacaaat cactaaatta aaaaatagct tttagtgtta actttttaat
     4201 tacatctata taagaagact gaaaatattt gttgggaata taagtatagt tattaaaaca
     4261 gaaaagtatt ttgttagaaa attaaaagta agctaattag aaagtggcct tattattgta
     4321 ctttataaga aggccactat ttttatttag aatgataatg tgtgcttgtc taacatttat
     4381 tttgtattta gtgcagaatt tattcagatt taaccttaag cttggctact gtttcttctt
     4441 caggagtaac ataaatagta ctgttagttt cataaataac catacctggt ttagaaccac
     4501 taggtttttt cacatttttt ctcttagtat agtctacagg cacctgagaa gaaagttttg
     4561 acttactaaa gaatgctgct agcatagctc cttcaaatac agtattatca gggacttcct
     4621 taccagcaca ctttattatt acatgagaac caggaatatt ctttgtatgc atccataagt
     4681 catcattatc agcaagtctt agtgtaagat agtcattttg cttattgttt ttaccaacca
     4741 atattttaaa tccatcagaa gacacaaatt catgaggttt tgtagatggg atagtatcct
     4801 ttttactgtt tttcttttga gtcttgatat atccaacctt agcaagttct tcttttatat
     4861 cttgtaattc agctaaattt tcgcagtttt caatacttaa aactatgttt tctaagtaat
     4921 ctatttcttc cttatttaag gaaatttgat gagatatctc gactttagca tgtttcattt
     4981 tgttatactt tttaaagtat ttttgagcat tttcagatgg tgtcaagttt ttatttaatt
     5041 ctattgttac atcattacaa ttttcatcat agaagttagc aatttcaata ctttccatac
     5101 ctttttctac catataaata tatgaagtaa tcaattctcc ttttatctta taaatgtctg
     5161 cattttcgga ttctgataat tcttcttctt gttttttaag tttattataa agtctatcaa
     5221 gcttaacaga tatactcttc tttaaatcag atgaacgttg attaattctg tctttaatat
     5281 ctt
//
LOCUS       DAEPLF010000225         5266 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEPLF010000225 DAEPLF010000000
VERSION     DAEPLF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.226"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            130..582
                     /gene="accB"
                     /locus_tag="KR314_003157"
     CDS             130..582
                     /gene="accB"
                     /locus_tag="KR314_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBH0329517.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            598..1959
                     /gene="accC"
                     /locus_tag="KR314_003158"
     CDS             598..1959
                     /gene="accC"
                     /locus_tag="KR314_003158"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBH0329518.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            1974..2828
                     /locus_tag="KR314_003159"
     CDS             1974..2828
                     /locus_tag="KR314_003159"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBH0329519.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKELLY
                     QILAMHKK"
     gene            2849..3793
                     /locus_tag="KR314_003160"
     CDS             2849..3793
                     /locus_tag="KR314_003160"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBH0329520.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            3982..4242
                     /locus_tag="KR314_003161"
     CDS             3982..4242
                     /locus_tag="KR314_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBH0329521.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            4395..4766
                     /locus_tag="KR314_003162"
     CDS             4395..4766
                     /locus_tag="KR314_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBH0329522.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIEDACKILSCNGYEVE"
     gene            4796..>5266
                     /locus_tag="KR314_003163"
     CDS             4796..>5266
                     /locus_tag="KR314_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329523.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLN"
BASE COUNT         2051 a          554 c          966 g         1695 t
ORIGIN      
        1 tactggttgt taatttaaaa ttaaaagata tattgatgtg ttgattctct agtcagcact
       61 ttttctttta taaatttgtt ttttgtgata taatattagc accaggtaat aatacaaggg
      121 ggtaacaaaa tgaacatcaa tgaaattaag gaattattaa aggccataga ctcaactaac
      181 ttagaatatg taaaacttga aagtagcgat ttaagattag aagtatctaa aaaagctcaa
      241 tctacaagtt cacctgtttt aagtgtacaa caagaatcag tagtggatct ttctttagaa
      301 aagccagttg taaacgatac acctgtcaca tctaatgaga atctatcagt agtagtagct
      361 cctttgatgg gaacattcta tgattctcca agtccagatg cagatagctt tgttaaagtt
      421 ggcgatgtag ttgaagaagg cgacacttta tgtatattag aagcaatgaa gcttatgaat
      481 gagataacaa gcgaaattaa aggtgaaata attgaagtat tagttagtaa tgaagaacta
      541 gtagaatata atcaaccttt atttaagata aaaccattgt agtaggaggt atttaagttg
      601 ataaaaaaaa tactagttgc aaatagaggc gatatagcag ttagaatcat cagaacatgt
      661 aaagaactag gtataaaaac tgttgctatc tattctgaaa tagataaaga ttgttttcat
      721 agatatatag ctgatgaatc tatttgtata ggaccaaata atataagtaa gagttataat
      781 aatatagaaa atataatcta tcttgctctt aaattaaagt gtgatgctat tcatccaggc
      841 tttggattct tatcagagaa tcctgaattt gcaaagcaat gtgaagataa taatattatt
      901 tttataggtc caacaagaga acaaatgatt ttgatgggag ataaatctag agcaagagaa
      961 actatgatgg aattgaatat ccctgttgtt cctggttcag aatctgtttt gaaaactaag
     1021 gaagaagctt tagaagttgc aagagaaatt ggatatcctg ttatgataaa agcctcaagt
     1081 ggaggcggtg gaaaaggtat gagaatcgtt agaaaagaag aagaactttt ttcaaatttt
     1141 gatatggcaa gttcagaagc tctagcagca ttttctaata gtgatttata tatggaaaag
     1201 tttatagaaa atcctagaca tatagaagta caagtatttg gagataaaca ttcaaatgca
     1261 atacatttag gtgatagaga ttgttctatg caaaggcgaa atcaaaaagt aatagaagaa
     1321 tctttaagtc catacctttc agatgaagaa agacaaaaac ttcataaaat tgcagtagat
     1381 atagtaaaag gagtaggtta tatcggtgca ggtactattg agtttatagt tgacaaagat
     1441 aaaaactttt attttataga gatgaataca agaatacaag ttgaacatcc agttacagag
     1501 atggtaacaa atcttgacct tataaaactt caaatatcta tagctaatgg ggataaaatt
     1561 ccttttaaac aagaagatat tacttttaga ggtcatgcta ttgaatgtag aataaatgca
     1621 gaagattcat caaagaattt tgctccatca ccaggtaaga tagaatcatt aaatttacct
     1681 ggaggttttg gtgttagatt tgatactttt gtatatgcag gatatactat accaccttta
     1741 tatgattcta tgataggtaa gttaatatgt tgggctgaaa ctagagaaga atgtataagt
     1801 agaatatata gagctctaga cgaaattatt gtagaaggta ttaatacaaa tgtagaattt
     1861 caaaaggcac ttgtaacaag tgaggagttt agagaagata ctcatcatac caaatttata
     1921 gaagatgtct ttatgaagaa agaatttgct acattataag gagtaaatag attatgataa
     1981 aaaaattttt atcatcaaaa gaatctaagt atgttacgat ttctttagat gacaacttta
     2041 aaaagaatag tgtagatgat aaattttgga cctattgcaa aggttgtgat agtcatgtat
     2101 ttagaaagga catagaagaa aattcatttg tatgtccaaa atgttcaaga cattatggct
     2161 taagagcaag aaaaagaata aatttattga ttgataaagg aacttttatg gagtttaatt
     2221 ctgatgtaga atttcaaaat ccactaaatt ttcctaaata caaagaaaaa gtagactctt
     2281 ataaggaaaa aacaaaagag tcagaagctg ttgtcacagg ttatggaaga ataaatggaa
     2341 ttaaaacagt tatttgtgta atgaatcctg attttatgat gggaagtatg ggttctattg
     2401 ttggggaaaa aataacttat tcaatagagt atgctgctga aaataattta cctataatta
     2461 tttgttctgc ttcaggagga gctagaatgc aagaaggtat ggtatctctt atgcagatgg
     2521 caaagacttc tcaagcactt tctaagcttg aagaaaaatc attgccatat atatcagttc
     2581 taactgaccc tactactgga ggagtaactg ctagttttgc tatgcttggt gatattataa
     2641 tttctgagcc aaatacgttg ataggttttg caggtccaag agtaatagag caaacaataa
     2701 atcaaaaact tcctgaagga tttcaaactt ctgagttttt acttgaaaaa ggttttattg
     2761 acatgatagt agatagaaga aaaatgaagg aattattata tcaaattctt gctatgcaca
     2821 agaaataggg cataaaggag gaactgtttt gattgaaaat aatagtgaaa aaataaaagt
     2881 cttagaaaat gatattaaac aattaataac tatttctaag gcaaataata tagatttaag
     2941 tgataaaata aattcactta atgagaaatt ggaaaagctt aaggaagatg ctttttcaca
     3001 tctatcagca tatgaaaagg taacactaag tagagatata aagagaccta cgacattgga
     3061 atacatagaa catatttgtt ctaatttttt agaacttcat ggagataggc tttttaaaga
     3121 tgacccttct atggttggag ggattggtca gataggaaag tttaatgtta ctatagttgg
     3181 acaccaaaaa ggaagagata caaaagaaaa tataaaaaga aattttggaa tgcctcatcc
     3241 agaaggatac agaaaagcat taagacttat gaaacaagct gaaaaattta atagacctat
     3301 aataacgttt attgatacat caggagcttt ttgtggttta gaagcagaag aaagaggaca
     3361 aggagaagct attgcaagaa acttacttga aatgagtaaa ctttctgttc cagtaatcac
     3421 ttttgtaatt ggtgaaggtg gtagtggagg agctttaggt atcggagtag gaaatgatgt
     3481 ttgtatgcta gaacattcag tttattctgt gatttctcca gaagggcttt ctagtattct
     3541 atttaaagat tcatctaaag ctaaagaagc ttgtgatgtt atgaagctta caagtaatga
     3601 tttatatgat ttaaaaataa tagataagat tataaaagaa cctcttggag gagcacaaaa
     3661 agatgtagaa gctgtttcaa aagaaataaa agcttatatt ttagaaagat taaatcatta
     3721 caaagatatg gataaagagg aaattatagc tcaaagatat aaaaaattta gaaatattgg
     3781 aaaatgctta tagaatataa agaaatttgt agttttttca taataaaatg gttaaaaaaa
     3841 ataaaggttt tcttaaaacg attatagaag tatattttta taaaagtata ctttaaatga
     3901 tgattgtaat tagtaaatgg caattatgat ttagtacttt aggtaatgga tactttaaca
     3961 aattaacaag gagatgttga aatggaggta ctaaaagttt catcaaaatc taatcctaat
     4021 tctgtagctg gagccttggc tggagtacta agagaacgag gcgttgcaga aatacaagct
     4081 ataggagctg gagctctaaa tcaagcgata aaatcaattg caatagcaag aggttttgtt
     4141 gcacctagtg gaatggattt agtttgcatt cccgcattta ctgatataga aatcgaaggt
     4201 gataagaaaa cagccattaa acttataatt gaacctagat aatatatctc tttattagaa
     4261 tttaattaag ttttaaaggt tggctataga aataggacac tatagtgaac cttttttact
     4321 ttatatgttt tattttatgt aacttttaat gtataatctt aagtaaacaa ataagattgg
     4381 aggaaatata cattatgaaa ttaaaattat tggtagaaga gtatgcagta tgtagattaa
     4441 ataatgacag taaaatacca acatggatag atacagagaa gttttactct ataactagaa
     4501 ctgatgatga attaagtgtt gtatgtttaa ataataatat tccttctgat gttaagtcag
     4561 aaaaggagtg gagaatactt aagatactag gtcctttaga cttttcactt gtaggaattt
     4621 tatctaagtt aagtagtttg ttagcagaca acaaaataag catatttgca atttctacgt
     4681 atgatacaga ctatatctta gtaaaagaaa aagatataga agatgcttgt aaaattttaa
     4741 gctgtaatgg atatgaagta gagtagttaa atacttttac atgaggactt aacttatgat
     4801 ttatataatc gaaattttaa aaataagtgt aataaatatt ttttgtttca taggaatatt
     4861 tattgtattt ggattgattt ttagcattac agaaaatcta aataataaat ttatcttttc
     4921 atcttttgga aaaactggaa ttattgtaac tggagctata ggaacgtttg ttcatgaatt
     4981 gagtcatttg ataatgtgct tgattttcat gcataaaata aattcagtta agtttttcag
     5041 accaatagaa agtaaaaatg atggtatact aggatatgta agtcatagct ataaaaaaga
     5101 taatctttat caaagtatag gaaatttctt tataggaatt gcacctatta taggtggaac
     5161 aataatgatt attttaattt ttaagatatt actgccagat agttatatga aagtgactca
     5221 aaatatagat ttaaagttat atgtaagtat gattaataat ttaaat
//
LOCUS       DAEPLF010000226         5078 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.227, whole genome shotgun sequence.
ACCESSION   DAEPLF010000226 DAEPLF010000000
VERSION     DAEPLF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.227"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(85..2679)
                     /gene="clpB"
                     /locus_tag="KR314_003164"
     CDS             complement(85..2679)
                     /gene="clpB"
                     /locus_tag="KR314_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBH0329524.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSDDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(2716..3312)
                     /locus_tag="KR314_003165"
     CDS             complement(2716..3312)
                     /locus_tag="KR314_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329525.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(3329..3733)
                     /locus_tag="KR314_003166"
     CDS             complement(3329..3733)
                     /locus_tag="KR314_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBH0329526.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNICFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            4100..4474
                     /locus_tag="KR314_003167"
     CDS             4100..4474
                     /locus_tag="KR314_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0329527.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            4480..>5078
                     /locus_tag="KR314_003168"
     CDS             4480..>5078
                     /locus_tag="KR314_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329528.1"
                     /translation="MDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGKTTT
                     LKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIMSKI
                     YKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATSSLD
                     PIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYIT"
BASE COUNT         1657 a          829 c          570 g         2022 t
ORIGIN      
        1 ataaaatatt tcttttccta tagtttataa ataaaaatca gctgagcaaa atatttagta
       61 tattatgctc aactgattct tttattattt aactaaaact tcaatatttt catcttttcc
      121 atctataact atagtacatc cattatacac atctccagca ataagtttct ttgcaattat
      181 agtttctaat gtatttccta tatatctctt aagaggtctt gctccatata cagggtcata
      241 tccttctctc accataacat cttttgctga atttgtaact tcaatagtaa tatctttttc
      301 ttttaatcta ttttttaaat ctttcataaa tatatctata attttcttga tattttcttg
      361 gtctaaaggc ttaaacatta taatatcatc tactctgttt aaaaactcag gtttaaatct
      421 atgcttcatt tcattcatta ctaaattgtt agtagtctct gttatatttc ctcctgcttc
      481 taatagataa ttacttccta tattagaagt cattattata agagtatttt taaagtctac
      541 tgtcttacct ttattatcag taagtctacc atcatctaat atctgtaaga acatgttaaa
      601 tacatcctca tgtgcttttt ctatctcatc aaatagtatt acagaataag gagcacgtct
      661 aactgcttct gtaagttgac caccctcttc atatcccacg taccctggag gaggtccaac
      721 tagtcttgat acagcatgtt tttccatgta ttcagacata tctattctta ttatattatc
      781 gtcactatcg aataaatttc tagcaagagt ttttgcaagt tcagttttac caacacctgt
      841 agggcctaag aaaataaatg aaccaattgg ttttctttca tcttttaatc ctgctcttgc
      901 acgtattact gcatttgaaa ctgctgtaac tgcttcatct tgtccaataa ctcttttatg
      961 aagttcatct tctagtttaa gtaatttttc tctttctcct tcaactagtt tagttactgg
     1021 tatacctgtc cactttgata caatgctaga tatttcttct tcagtaactt cttcttttaa
     1081 aagagaattt tcagaaccat cattcatatt ttcttcgtac tgctttattt gttcttctaa
     1141 cttaggtatt tcaccatatt taacctctgc tgctttgttg aaatcatatt ctctttcata
     1201 cttttcagct ttacctttag cttcgtctaa ttgagccttt aagtttttaa tatctaatat
     1261 ttgactcttt tctttttcat attttgctgt catttcatca tttttagatt ttaactctgc
     1321 tatttctttt tgaatatcat ccaatcttga tttgctttta tcatcatttt ctttaagtaa
     1381 tgcttctctt tcagtttcta atgtaaataa ctttcttcta acaacatcta attctgttgg
     1441 caatgaatct atttcactac gaatcatagc tccagcttca tcaattaaat caattgcttt
     1501 atctggtaaa aatctatctt gtatatatct atgagaaagt tttgctgctg ctacaatagc
     1561 attatcatgt attcttactc catgatgtat ctcaaatctt tcttttaaac ctctaagtat
     1621 agatattgtg tcttcaacag taggttcttc tgcaattaca ggctgaaaac gtctttcaag
     1681 agccttatct ttttctatat actttctata ttcatcaaaa gtagttgcac caatacagtt
     1741 caattcacct cttgcaagca taggtttaat aagattacct gcatccattg aaccttctgt
     1801 tttaccagct ccaacgatag tatgaatctc atctataaac agtataatct ttccatcaga
     1861 actttgaact tcttttaaaa ctgcctttaa tctttcttca aattcacctc tatattttgc
     1921 accagcaatt aaagctccca tatcaagtga gaatacaatc ttatctttta aaccttctgg
     1981 gacatctcct cttactattc tctctgctaa cccttctaca attgcagttt tacctacacc
     2041 tggctcacca atcaatacag gattattttt tgttcttctt gataaaattc ttataatcct
     2101 tcttatttct tcatctctcc caattacagg gtctaattta tttttcttgg ctaaatctac
     2161 taagtttgtt ccatatctag caagtgcatc atatgtgcct tctgggtctt gtgtttctac
     2221 tctttgacta cctctcactt gtgataaaat atttaaaaag tcattcttat ttatattata
     2281 ttgttttaat attttaccaa cagctgattt tgattctgtt tccatcattg caagcataac
     2341 atgctctaca cttatatatg aatctttaaa ttctttagaa atactctctg cctttataag
     2401 cacctcattt atttttctag tagctgatac tccttgtgag tcagcacctt ctccatatac
     2461 ttttggaagc ttatcaattt caaagtttac agtatttctc aaagaatcaa ttgatatatt
     2521 cattttttcg attatatttg gtatcaatcc atcctcttga ttaataagtg ctgagaaaag
     2581 atgaattaca tctacttgtt ggttatgatt ttttactgct tcgttataag cctcatttaa
     2641 acttttttga actcttaaag tcattttttc tacgtccata taaatccctc cattttaata
     2701 ttatatccaa ataaattaaa ttctctcatt tataattatt gattttctaa gctctaaaag
     2761 actatcaagt aaattctcgt ttttttgtat atcgaaactc aatgttaaat taaatgttcc
     2821 atctgaatta aaaaaatcga atacttcttt tgattttgct attaaataca agtcttcatc
     2881 tataagtctt ttttcaccta ttaataaata ctgacattta tctataaaac tctttttaaa
     2941 atcaatagtg tatagatttt ctaaaatatt atattgataa atagcaatat tattaaacac
     3001 ttttaatatg tttgaattta caaaattgta aatttgatta ttgttataca gtgatatttg
     3061 ttcgtatata tattccctaa ataaaggatg aatgttttgc ttaagtttta aagtaacttc
     3121 agctggtatt tcaaaataaa gcttccagta cttagagtta tttttttcta aaataaaagc
     3181 tactttgttc aaactggtag aactaatctc ctttagtgta caattaaaat tatagtgtct
     3241 caaactagtt ttttcattct caaatacata gtcaaaccac tgtagaacat ttctatcaag
     3301 ttttaaactc atataaaaac cttcttcctt atttactaac cttattaggt ctttttatct
     3361 gattatattc tgctaataaa ctttctttat cagctctact acctttatat gctatatgtg
     3421 gatttatcat atactcacca tttttcactc tttgtaaaaa atccattttt ttcaactccg
     3481 taaacaatga agataaagaa gtttttggat attcaaattc acaagataat tcatataatg
     3541 tttttctaat cttattattt ttatccatag agtttattaa ttttaatgga attaaatccc
     3601 tcttagtata cattccttct actactttaa tataattctt aaaacaaata ttcatatact
     3661 tacttttatt cataactaaa cttttgctct ctgtatattc ctgatttaaa tcctttttta
     3721 atgtcttatc caaattaaat atctcttctc actctcctta tctctaattg ataatataaa
     3781 tatattacaa aaaaatatgt atgtcaaaac tgaaaatgca aaaagtttac cttataagta
     3841 aacttttgtt tagctataag ctaaacttta ttattttttt aatttagtat ttccaattta
     3901 ttggcaggat ttttgaataa tatttataaa ttttttaaaa aaatttaaat ctatttaata
     3961 tggcagcctt tcataaaata cattacattt attaaatgta taatattggt aattagtttt
     4021 ttctaaaatc agttgacata ttatatatat atttatataa tgtgtatata ctatatatac
     4081 aatttaagga ggtgacttat tgaatatagt tattagtaat tcgagtggga agccaatata
     4141 tgaacagatt actacacaaa ttaaaagtat gattataagc ggagagttac cagaaggctc
     4201 agctcttcca agtatgagac tattggcaaa ggaacttaga ataagtgtaa ttactacaaa
     4261 acgagcatat agcgatttag aacgagacgg atttattgag acagttactg gtaaaggcag
     4321 ttttgtatct agtaaaaaca tggattttgt caaagaagaa caatttcgtt taattgaaga
     4381 atacctacaa aaagcagttg atacagcaaa aagtagtgat attacatatg acgaattaaa
     4441 agatatttta tttttattat ataaaggaga atgataagta tggatgcaat aactataaaa
     4501 aatttaaata aaacttataa agattttagc ctacaagata tatctttttc agtaccaaaa
     4561 gggagtgtaa tgggatttgt aggtgaaaat ggtgcaggaa aaacaactac attaaaagca
     4621 attttaaatt taatatctta tgatagtgga aatatagaaa tatttggtct tgatagtaaa
     4681 aaaaatgaaa aagaaattaa agagcaaatt ggggttgtat ttgagggaag caactttcat
     4741 gaaaatttaa acactgacca tgtttcaaaa atcatgagta aaatctataa aaattggaat
     4801 gacacattat ttaaagacta cttaaaaaaa ttgagagtac cagacaataa attaattaag
     4861 gagttttcaa aggggaataa gatgaaactt agtattgctg tagccttatc tcataaacca
     4921 aaattattaa tactagatga ggcaactagt tcacttgacc ctatcgttcg tgaagaaata
     4981 ctagatatat ttttagattt tatacaagat gaagaacact ctattatctt atcttcacat
     5041 attacaagtg atttagataa gatagcagac tatattac
//
LOCUS       DAEPLF010000227         5074 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEPLF010000227 DAEPLF010000000
VERSION     DAEPLF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.228"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            9..911
                     /locus_tag="KR314_003169"
     CDS             9..911
                     /locus_tag="KR314_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBH0329529.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIATF
                     GKLLIPFIMAGILGIVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            1520..2533
                     /locus_tag="KR314_003170"
     CDS             1520..2533
                     /locus_tag="KR314_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH0329530.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKTFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFEIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            2889..3458
                     /locus_tag="KR314_003171"
     CDS             2889..3458
                     /locus_tag="KR314_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBH0329531.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKSIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(3795..4967)
                     /locus_tag="KR314_003172"
     CDS             complement(3795..4967)
                     /locus_tag="KR314_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH0329532.1"
                     /translation="MIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPII
                     IGLNFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQY
                     LSNKIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAIL
                     ANIGVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIES
                     YLEIEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNID
                     IDNHLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKV
                     NPINRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
BASE COUNT         1855 a          510 c          838 g         1871 t
ORIGIN      
        1 ttggaaatgt gttgccacaa gattattatg gattaatagt gattttaggt gttattcttg
       61 gagttatggg ggcattttac aactggttta ctctatatgt gcagtcttta tacaagaaga
      121 ttcctaaaat tgctaccttt ggaaaactgt tgattccatt tataatggct ggtattcttg
      181 ggattgttat gcctgatgtt ctggggagtg gacacaattt agttgaagaa cttacaagtc
      241 atgaatatat ttttggaatg gttgttttga tttttgttat acgttttata ttttcggctg
      301 ttagttttgg gtcaggtgca ccagggggaa tattttttcc attgcttgtt ttaggagcat
      361 ttataggtgg aatatttggt atggtaggtg ttaaagtttt tggaatgaat cccgattatg
      421 taaataactt tgtgcttttg gctatggcag gatattttac agctattgtg cgtgcccctt
      481 tgactggtat catattaatt tttgaaatga caggttctgt tagtcaaatg ctttcactat
      541 cagtaatttc aattgtggca tatattgtgg ctacacttat gaagtctaaa cctatttatg
      601 aaagtcttct tgagaggtta cttgaaaaaa atggagaacc tgtacataaa gagaggggcg
      661 aaaagatact agaccagttc tctgttatgt ttggctctcc tgttgcgaac aaggcaattc
      721 aggatgtaca gtggcctgaa aattgtcttt tggtagcaat tcaaagaggt ggagaagaaa
      781 ttattcctag aggtaagaca attcttagac caagtgatgt aattgtgact atgacagatg
      841 agagagatag tggtgctgtg tatgataaga tggaaaattt gtgtaaagaa catacgtcac
      901 atatttttta gggataatga tgaatgttta ataatgatag aatatttaat gaagatttga
      961 ttaaaaaatt ggtaatttga aagagagtta aggaattaat agaaaatgaa aatgtgatat
     1021 cttagatgta aaagagtttg aagaaaataa ttgtatatat ttgttaatga gattatatgg
     1081 actaatggta tatttatact gtgctaaaag ttgcactatt gtatgaggaa tgggaataaa
     1141 gggtttgaaa aaataggtag aagatttttc agatattctt attgggatat tttaattaat
     1201 atattttaat tgcgatattt tgggaagaat agaatttatt tataaatgaa aataattaaa
     1261 aggggttaag gttgtattag ccccttttgc ttatagagtt tggaaatggt atcttgatag
     1321 cagttgtttt atgcttatta tattttatta catattttaa aattaacctt atcactccta
     1381 aatcattatt ctatcaactt ttaaaacact ttcaagacaa tcacctattg aaaacaaagt
     1441 taattaaaaa cctacatatc agtttagtat tcgtgtagat gattatataa atataaaagt
     1501 ataagaaagg tgagtatctg tggttagttc atttttaaat gatatcttta gaacaatttt
     1561 agtaagttgt atttttatag ttattttatt agtctttaga ataactttat ttaagacttt
     1621 cagtaaaaaa tttaattact atatatggct tattgtgatt attaagctat cattaccatt
     1681 tatgaattat acttttatat ttaatgagtc aaaatataat ggtaatatta ataagataag
     1741 tttaggcaat tttaatagta tttctattgt atatagtact gttttagtct ctgtttggat
     1801 tgctattacg atattttatt tagtttatac tttattgaaa tatattaagc ttaaaaatat
     1861 gataaatgat ttgtcttatg atgttgagga tgataagata aaaaatcttt atgaaaattt
     1921 attaaaagag cttaagataa ataaaaaaat acggcttaaa tattcatatg aagttgaaag
     1981 tccagtattt tttaattcgt gtgtcatttt accaccatgt gattatacat taaaagagtt
     2041 aaactggata tttaggcatg agttgatgca ttttaagagt aaagaccttt acattaagta
     2101 tttggtgata tttttaaaat gtgtttattg gtttaatcct tttgtgtata taatggagaa
     2161 gtttatagat ttggattgtg aattttattg tgatgaaaga gttttgaaga attgtacgat
     2221 agaagagaag caggattatg cattgacaat aattagtgca atgagaaaag gttcaaatag
     2281 ttcaaataaa tttgtggctg gattacataa ggagtcagat attaaaaaaa gagtgtttag
     2341 tatgtttaat gaaaagtaca gaaatggtat tttgatagca gttattttat gtttattatc
     2401 ttctattaca tattttgaaa ttgatttaat tactactaaa tcaatatttt atccactttc
     2461 aaaacaaaca cctattgaaa atagaactaa ttcaaaacct acatatcaga ttaatgttta
     2521 tatagataat taaaaaatac aaagtttaac aagagattaa tataaataat tttaataagt
     2581 atctttcaca tatgttaaat ttactttaat tatattaaag ccaatgaatt attctttaga
     2641 agctccaatg ccagtaaata caggtaaaga aaaatctttt actacaattg taaattaaaa
     2701 aatattctaa tatttaaaga aatgcaaaag tgtaagaagg gtaaaaatct gttataatac
     2761 attaaaaact aaatttagaa aaatttacat aattagaaaa atgatttgat aaaaaataaa
     2821 gttaaatatt aactaagtaa atttgaaaaa gaaaaaatat aaagtacaaa tttaaaagga
     2881 ggtaactaat gaaaaagaag ttggaagata tgactttaga ggagttatgg aacttgtttc
     2941 ctatattttt gactactcac aaagaatgtt ggaaaaattg gtttaaagaa gaacaatata
     3001 gactaactgg aatattgcca atgcaaaaaa ttgttagaat aagtcatatt ggaagtactt
     3061 ctgtgagtac gatttatgcc aagtccattg ttgacatact gattgagata attcctgatt
     3121 ataatatgaa agatattaaa gatttgattg agtcgaatgg atatatttgt atgtctgaga
     3181 gcagtagtag gctttcactt aataaagggt atacagtaga tgggtttgaa gaacgcgttt
     3241 ttcatttgca tataaggtat gtaggtgata atgatgagtt gttttttaga gattacttaa
     3301 ttgaccaccc agatgttgca aaggagtatg aaaaacttaa gttggacctt tggaagaagt
     3361 atgagcataa tcgtgatgct tatacagata gtaaactaga ttttgttagg gagtttactg
     3421 aaaaggcaaa ggtgttgtat tctaatagat ataattagaa ataggtacaa ttagatattt
     3481 atcttatata tcgtattata ttttttaaga aagtaattaa tagaaaaagg ggttgtttca
     3541 aaatggagag ataaatccct ttttcattgt tgaaatgata aaaatatatt tgatttcatg
     3601 taaaaaagag tgcctcatca actaaaaagt ttattttgag acacacacat ttttattaag
     3661 gtaaataaaa atatcacatt tgattaaatt ttaagttaat gaatatatgt gtaaatattt
     3721 aattataagt tattataatt ttacatagta taaaacaatc taatttgtat tatataagat
     3781 aatctaattt tagactaaaa taatctaata taaagaaata gagaaaattc attctcagta
     3841 taagagatat ccaattttcc attgtactta ttaacaattt ctctaatatt ctcaatacca
     3901 tatccgtgag aattactatc ttttttatct gaaataaata gttcttgttt tctattgata
     3961 ggatttacct tgttgtttgt caataaatag ctaaaatact ccttctcaca acgtgccttt
     4021 aataccactt ttctatttga caaatcagga attttcaaag atgcttctat agcgttatct
     4081 aaagaatttg aaaaaatagt acataaatct ataggttcta tacccaagtg attgtctata
     4141 tctatattaa agtgcacatc aacaccattt tcttccaaac ttgtatgacg aagatttaaa
     4201 attgagttaa gagcttggtt tttgcaaaat tgtttgccac taagagtacc tatacctatt
     4261 tttaactgat taaaatactt tttagctcca gcaatatctt caatttctaa atacgattca
     4321 atcatttgaa agtgattatt catatcatgt cgcattttgc gtatttgaat ttgttgattt
     4381 tctagtaagt tatagtattt ttcttttagg tgatttgctt gtttttctat cttcatcttt
     4441 acattttcaa tcaatggctg taaaagaagt atcacaccga tatttgccag aattgctgac
     4501 aagatgatta gataagtgcc ataaggtaag ttgcctaaga taatattctt attactaccc
     4561 atagggatta aattagtagg tggcagaata atagcaatta atattgataa aaatgaagat
     4621 atacatacca tatcaataaa aatccatatt ttatttgata agtattgttt tatattgacc
     4681 aatctattct ttaggaaaat atgtattaaa aaccaaaata ataatttaat tagagcagaa
     4741 attatctgga ctattgaaag tattaaaata tattcttttg agtgtataag ttcctcatat
     4801 atagggttgt ttataaaaag aaaattaagc ccaattataa taggataaag tatcatgaca
     4861 actgatatta taggaattaa tttgcttttg ctacatatta taagtagtat aaaatatccg
     4921 aatagtgtaa acaaaatatt tacggggtcg gaaagccata ttatcatttg actaataata
     4981 gagtagccca aaatcaatat gattttgaaa atccaatgat tcctaaagtc tataaaatag
     5041 gaagtcaact tataaagaaa aaaggctatg aaaa
//
LOCUS       DAEPLF010000228         4979 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEPLF010000228 DAEPLF010000000
VERSION     DAEPLF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.229"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..912
                     /locus_tag="KR314_003173"
     CDS             <1..912
                     /locus_tag="KR314_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH0329533.1"
                     /translation="IDVFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWIL
                     VNKDDETFYNKIQEKCKYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCW
                     IKSKNINKYIVDKSEYRLIYSNDIDNENTNKKILDEIIGLYKTKLENRRECKSGIRKW
                     YELQWGREKLFFERKKIMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEY
                     LVGILNSSVYDKYFKITAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISIL
                     LNKSIDKGKVEKLQIKMDNLIMDSLGI"
     gene            1144..2355
                     /locus_tag="KR314_003174"
     CDS             1144..2355
                     /locus_tag="KR314_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBH0329534.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            2547..2807
                     /locus_tag="KR314_003175"
     CDS             2547..2807
                     /locus_tag="KR314_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBH0329535.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            3160..4872
                     /gene="ptsP"
                     /locus_tag="KR314_003176"
     CDS             3160..4872
                     /gene="ptsP"
                     /locus_tag="KR314_003176"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBH0329536.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASIHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT         2081 a          444 c          887 g         1567 t
ORIGIN      
        1 atagatgtat ttaaaatcaa aaatgaggat atatgtataa ataaatttga aacattggaa
       61 gaacttctaa agagtagcaa atttgaacat tttaatataa atcagagatt gctatctgac
      121 gaatggatac ttgtaaataa agatgatgaa actttttata ataagataca agaaaaatgt
      181 aaatatagct tggaagatat agcaataagt tttcaaggaa taattacagg gtgtgataaa
      241 gcgtttattt taagtaaaga tgatgtaaag ttgaaccttg tagatgataa atttttaaaa
      301 tgttggataa aaagtaagaa tataaataaa tatatagtag ataaaagtga atataggcta
      361 atatactcaa atgatataga taatgagaat acaaataaaa agatactaga tgaaattata
      421 gggctatata aaacaaaatt agaaaacaga agagaatgta aaagtggaat aaggaaatgg
      481 tatgaacttc aatggggtag agaaaagctt ttctttgaaa gaaaaaagat aatgtatcct
      541 tataaatcaa atgaaaatag atttgctata gattatgata acaatttttc aagtgcagat
      601 gtatattctt tttttataaa agaagagtat ttagataaat tttcatatga atatctagtt
      661 ggaattttaa attccagtgt ttatgataaa tactttaaaa taactgctaa aaaaatgagt
      721 aaaaatatat atgattacta tcctaataag gttatgaaga taaggatttt tagggataat
      781 aattatgaag agatagagaa tttatcaaag caaataatat caattttatt aaataagtct
      841 atagataaag gaaaagtaga aaaattgcag ataaaaatgg ataacttgat aatggattct
      901 ttaggtatat aagcagttgt aagtattaaa aaattataat cctttaattg tttatttaaa
      961 tttagaaata aaaagttagc catataaaaa atattaaaaa tatatacttt tcaatatttt
     1021 tttgataaaa taagaatgaa tttgtgcaat tttatttttc tccaataact aaaaatagtt
     1081 ttttgaatta atatttgctt aagaataaaa atcagaaacg tttttgatat agtaggtgat
     1141 aatatgaaag gattagtttt agaaggtgga ggaacaaaag gagcctacca aataggtgca
     1201 tataaagctt taagagagct tggtatagag tttcaaggag taactggcac atcaattggt
     1261 gctttaaatg gagcatatat aattcaaaat gatattgaag taatggaaga tatttggtta
     1321 aattatgact atactcattt tatgaatatt gatagagaaa cctatgagag gtttaaaaat
     1381 gtagatttca ctactaaaaa tctcaatgca gtgtttgagc ttataaataa agctagaaaa
     1441 aatgaaggta tagatataag cccattaaga caactaatgg agacaacatt aaatgaagac
     1501 ataataagga aatcaaaaaa agactttgga cttgttacgg tctggtggga taaaaaaatt
     1561 aatccatatc cattgtatat cgaagatata cctaaaggaa gattgattga ttatttaatt
     1621 gctagttcca gtttgcctat ttttcaaacg aacatgatgg atgataagtt gtatcttgat
     1681 ggaatgtttt gtgataacat gccaatagga ctcttggaag acaaaggtta tgaagatgtt
     1741 gttgtaatta ggttgattga tgattttatt gggaaaatga atctaaatcg acatcaaaat
     1801 ataaatataa aaactataat accatcagaa tatctaggag gttctcttaa taaagataga
     1861 gataatgtag aaaaaaacat ccgactaggt tacttagata ctatgaaagc atatgaaaga
     1921 tgcactggaa ttaagtatta ttttgatgta gagtataaat ataatgaaga atattgtttt
     1981 gataaattta gaagtatgag caatgaaaca atacaatata tctgtaattt attgaatata
     2041 aaaagagatg cttcacttag aactgttatg gaaagtgtaa taccaaagct tggtgaggtg
     2101 cttggattgc caaaggactt ttcatataaa gatttgtttt actgtatata tgaaaagaaa
     2161 cttgaagaaa ataatataaa caggataaaa ttatatgatt ttaacaaggt tatagatgtt
     2221 gtaaatacaa atattgattc taatagaaat ttagcttcta attttgaagt tagacctaaa
     2281 attagtatta aaaagaacaa aaacacaaaa ttattgacaa attgtattat aaaagacata
     2341 aaaaatcaaa gttaaataaa aaacaaaaaa aaatttgtaa ttatactcga aaaaagtgta
     2401 tattagtagt ataatatttt tgtagcacta gtgggaacaa aaaatgacca taggacaaaa
     2461 aagtaccact tagtaccatt aaagtaaaaa acaggaaaac aattttaaaa aatataaaat
     2521 gtaaagttta taggaggatt taaaaaatgg aaaaagtagt tagtatcaaa aatgcaagtg
     2581 gattacatgc tagaccagca ggaatgtttg ttaagaaggc agctgaattt aaatcaacag
     2641 tagaggttgt agcaaaagac aaaactgtaa atgcaaaatc aataatggga ataatgagtt
     2701 taggtttagc tcaaggagaa gaattaaagt tagttgtaaa tggagaagat gaagaagtag
     2761 ctttagctgc aatggttgaa ttagttgaaa gtggatttgg agaatagata taataatttt
     2821 ttaaaatttg atatttagaa tctaatatta cttaaagtaa agtaacagag aagtattaaa
     2881 catatacaga ccaacatttg tagatattta atttctttgt tactttcatg tttatttaat
     2941 cagaagatta agactattgt taattgggta taaaaatata aatattttag aaaatgaaat
     3001 aagatatttt taatcataaa ttcagagtaa aaatacagta acaaacagtt tataaaattt
     3061 aataaaaaat tataagtaaa taaaactata aaaatgtaaa ttatttatat aaatttatag
     3121 agtttatgtg ctagatgata ttttttagga ggtagggtta tggcttacaa aggaataggt
     3181 gcttctcctg gtgttgcttt agggaaagcg ttagtagttg aacatagtga attagtaatt
     3241 gaaaaaaaat ctatagataa tgttgaagct gaaattgcta aattagaaga tgcagttgca
     3301 gtttctaaag aagaattagt aaaagttaag gaaaaggctt ctgaagaatt aggagcagag
     3361 gaagcagaaa tatttgaagc acatttatta gttttagaag acccagagtt aattggttct
     3421 gcaatagata aaattaaaac tgaaagtgta aatgcagagt atgctttaaa tgaaataaaa
     3481 gagatgtttg ttagcatgtt tgagtctatg gacaatgagt acatgaaaga aagagcggca
     3541 gatataaaag atgttacaaa tagaatactt agacatatat taggaattaa agttgttgat
     3601 ttatctgctt taagcgaaga agttgtatta atagcacatg atttaactcc atcagacaca
     3661 gcaactatga ataaaaaaat ggttttagga tttttaactg atataggtgg aagaacttct
     3721 catactgcta tcatgtctag aactttagaa atagctgcaa tcgttggatt aaatgatata
     3781 acttcaaaag ttaaggatgg agattttgta gttttcaatg gagacacagg tgaagtaatt
     3841 gtgaatcctg atgaagagac tataaataaa tatactgaat taaaagctaa gtatgaagat
     3901 gagagaaaag cattacagct attaaaagga aaaccatctg taactttaga tggaaaacat
     3961 gtggaattag ctggtaacat aggaacacct aacgatatag aaggtctaat aaagaatgat
     4021 gctgaaggtg ttggtcttta tagaacagaa tttttataca tggatagaga ttctttccca
     4081 acagaagaaa tacaatatga agcatacaaa gctgtattag aaggaatgga tggaaagcct
     4141 atagttataa gaacattgga tataggtgga gataaagaat taagttattt aagtatggaa
     4201 cctgaaatga atccattctt aggttacaga gcaataagac tttgcctaga tagaaaagat
     4261 atatttaaga ctcaattgag agctttatat agagctagta ttcatggaag attaagaatc
     4321 atgttcccaa tgatttcatc tttagaagaa ttgttacaag ctaaagaagt tgtaaaagaa
     4381 gttttagcag aacttgattc tgaaggtgtg gcttatgcta aagatgtaga aattggtatg
     4441 atgatagaag ttccatctgc tgctgtaata tctgatgtat tggctaaaca cgtagatttc
     4501 ttctctatag gaactaatga tttaattcaa tacacttgtg cagttgatag aatgaatcaa
     4561 aagataagtt atctttacaa tcaatttaat ccagctgttt taagacttat aaagactgtt
     4621 atagacaatg cacataaaga aggaaaatgg gcaggtatgt gtggagaatc tgccggagac
     4681 caaaaaatga taccaatact tttaggtatg ggattagacg agttctctat gtctccaata
     4741 tcaatacttc ctgctagaaa gttaataact tcagttaaag agtcagatat gaaaaaatta
     4801 gctgatgatg tacttaatat gggtactgct gaagaaataa aaagctatat agaaaagact
     4861 tttaatatat aattaatgtt atatagttac taaaattcat ttttctaaat aacaaaatta
     4921 tattttacga attaaaaagg agatgtttca aaataaaaat ttgaagcatc tctttttta
//
LOCUS       DAEPLF010000229         4974 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEPLF010000229 DAEPLF010000000
VERSION     DAEPLF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.230"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            88..813
                     /locus_tag="KR314_003177"
     CDS             88..813
                     /locus_tag="KR314_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0329537.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCKYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            800..2044
                     /locus_tag="KR314_003178"
     CDS             800..2044
                     /locus_tag="KR314_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0329538.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMKNIDYGQKILSKYNYDKSIKINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            2124..2801
                     /locus_tag="KR314_003179"
     CDS             2124..2801
                     /locus_tag="KR314_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329539.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            2802..>4974
                     /locus_tag="KR314_003180"
     CDS             2802..>4974
                     /locus_tag="KR314_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0329540.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDQNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIKKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGESYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGFGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNDFNFYDEISSSESEKQDLKVIKIF"
BASE COUNT         2079 a          464 c          856 g         1575 t
ORIGIN      
        1 gaaatatttt agtatccagg gtaattgtgt ataataaaac ttgtatagat agatagtatg
       61 aaattaagca gatagtttaa aattaaaatg aaaagaaagg aaatagttat gttaaagttg
      121 ttgatagttg aagatgatag tactatatct tttggaataa agtatgcttt agaacaagaa
      181 ggatttagta tagatatatc aaaggattta tctagtggaa aagaaatgat ttcttctaat
      241 gaatatagca tgatactgtt ggatgttaca ttgccagatg gaactggtta tgagctttgt
      301 aaatatataa gaggattctc tcaggttcct ataatattct tgactgcttg tgatgaagaa
      361 gtaaatattg taatgggtct cgatataggt ggagatgatt atataacaaa accttttaga
      421 attagagaat taatatccag aataaaagct gttttaagaa gaaaaggtaa tacttcagaa
      481 gaaaataaga aaatacttaa atttggagat ttaagtatat atacattaga agctagagta
      541 tataaaaatg ataaagaaat atttctgaca tcagttgaat ataaacttct tttaattctt
      601 atacagaata aaaatactgt tttaagtaga actcaaatat tggaaaaact ttgggatgta
      661 acttatgatt ttgtaaacga caatactcta actgtttata taaaaaggct tagagaaaaa
      721 ataggtgatg atttatgtga accaatctac atagaaactg taagggggat tggttataaa
      781 tggataggaa gtgaaaatag tgtttctata taatccagaa gtgaagaagt ttttaagtaa
      841 gtatgttact ttgatgttta ttgttatcat aatatctatt ggatttagca taatcaacgt
      901 gtctttaact aaagagatgg ttgtaagaaa taatcaagct ataataggga ctttatcgag
      961 taagtatcca aacttagaaa gtcagatagt agacataata actcaaggga agtctatgaa
     1021 gaacatagat tatggtcaga aaatattaag taagtataat tatgataaat ctattaagat
     1081 aaactctgag cctattattt ctaaattagt tttagagact atcaaaataa atataatatt
     1141 agtatgtata atatttattt tgatatttgt gttggttgtt agatatttta cgtcaattta
     1201 caatgattta tcagatatga ctaagtatgt ttactatagt tcagagggta agccatttga
     1261 tatgaaaaac aagaatcaag aagggcagat aggtcttcta aaaactgaac tcttaaagat
     1321 gacgacaatt cttaatgaaa aggttgaact tttaaaaaga gaaaagatat ttttaaacaa
     1381 tacaatttcc gatatatcac atcaattaaa aactcctatg acttctttaa taatgttgaa
     1441 tgatttatta tataatgatg taccatacga agtaaaaatc gattttttaa ataagataaa
     1501 aaatcaatta aatagaatgg attggttgat taaaagcatg cttaaattat caaaggtaga
     1561 agcaaaagtg ataaatttta aaaaagataa ggtaaaattt agtgaactca tacatagagc
     1621 aatgctatct atgaaaatac ctatggagat taaaaatcaa aaactgacta tagagggaaa
     1681 tgataatgta tcttatattg gtgatattga ttggtctgtg gaagcactag tgaatataat
     1741 caaaaattgt gtagaacata ctcctgaatt tggaaatata attataacct ataaagaaaa
     1801 tccattattt tctgaactaa taataaaaga tgagggtgaa ggtatagata aaaaagatat
     1861 accacatgta tttaaacgat tctatagagg aagaagtagt tcaaaagaag atagtgtagg
     1921 aataggtctt gcaatgtcaa aatctataat agaaagccaa aatggcgata tatatgtaaa
     1981 cagcgaaaaa ggtaagggta cagaatttca tataatattt cataaaatgt atgatagtga
     2041 ctaatctgta atatttaatt cactttatag taatagccaa aacctaatat atgaagtata
     2101 gaatataaat attggaggta gttatggaaa ttttaagagt agaaaatcta actaaaagtt
     2161 atggtaaaaa tgaaacaaaa gttgatgcta taaaaaatgt aagtttatca gttgaaaaag
     2221 gaacgtttat agcaataaca ggtccaagtg gaagtggtaa aagtacctta ttacatttat
     2281 taggaggagt tgacagacca actagtggta aggtttatat aaatgatgtt gatatatata
     2341 atttaaaaac taaggatttg gcgatattta gaagaagaaa tataggtctt atatatcaat
     2401 tttacaactt aattccagtc ttaaatgtga aggaaaacat attgcttcca gcagagcttg
     2461 acaatagaga tattgataga gaatattttg atgatttaat gaaaacatta ggtctaattg
     2521 atagagaaaa gcaccttcca aaccaattat caggaggtca acaacagaga acctctatag
     2581 gacgtgcatt aattaataga ccttctataa tattagcaga tgagccaaca ggtaatttag
     2641 atagtaaaaa ttcaaaggaa atattagaat tgttaaaatt gtcagtaaaa aaatacaatc
     2701 agacactaat aatgataact catgacaaaa atatggcact tcaagctgat agaattataa
     2761 gcatagaaga tggaatcata aaaagtgatg aggtgatgta gatgaattta tatacatcac
     2821 taaccattag atacttaaaa caaaataaaa gaagaaccat tgtaaccata atcggtataa
     2881 tactttcaac agctttaatt tgtggtatag gaaatatatt tgaaagtttt atggattatc
     2941 aaatgagaga aactataaag aatgatggaa gtttccatgt tacattttat gatgtaaata
     3001 gaaaaaatgt ggaatatgta acgaaatcag ctgaaataga aaaacatgca tttaccaaac
     3061 aacttggata ttctaaatta gaaaatagtg aaaatgctat attaagtata aagcaatatg
     3121 atcaaaatgc aataaacgga tataaagttt ctataaaaga aggtagattt ccagctaagg
     3181 aaggagaaat tgttttaagt gaaagtatta ttaatcttat agatgataag ttaaaaatag
     3241 gagataagat aactttaaaa gtaggggata tgtttgactc aagcaagaaa aagattgata
     3301 gtatgacatt tcatgaaggt gattatatag caaatgaaaa agatagaact tttaaagttg
     3361 ttggtataat taaaaaacct gggtttgaac gttataatgg aattgcaaca gctatagcct
     3421 actttaatcc tatgaataat tacaatgata caattaatgt atcagtagca gtaagaaatc
     3481 ctaaagatgt gtataagata agtaataaaa taagtaaaaa tatatattca aataaagata
     3541 aagaagcagt ttctgatatg ataaaataca atgaacattt attgaggtta caaggtgcta
     3601 gtaagtattc aaatataaat agctctataa agtctataat tatagcagta actattttag
     3661 taatcatatg tacaattgct acagtgtaca attcctttag tatttctata aatgaacgaa
     3721 aaaaacagtt tgggatatta aattctatag gtgctacatc tagtcaaatt aaaagattag
     3781 tttttataga aggtattata attagtttaa ttggtatacc aataggacta atatctggaa
     3841 cagtagctat agacctattg tttaaaatta taaataagta ttttactgaa tcggttgtta
     3901 ctaagatgag tttgcagata gtgtataatc caataataat tattgttagt ataataatag
     3961 ttttgcttac aatctttata tcaattctac tacctgcaat atcggcttct aatatttctc
     4021 cccttaatgt gattaaaaat actggagagt ataaagtggg caaaatcaaa agctcaagac
     4081 ttattaaaat gatttttaaa actgaaggag tacttgcata taaaaatatt aggagaaata
     4141 gaaaaaaatt tataataact cttttttctt taatggtaag tataataata tttatatctt
     4201 ttagtggctt tacattactt ttactaagag gtgaagaaat tagaaattct caaagaaaat
     4261 atgatttgta tttaactgca aagaacacag ctaggtctgt agaggatact ataagtgaac
     4321 tagaagatat tgatgggata aaaaactttg aattggcaac aggacaatat gtgtctataa
     4381 gagtaagtga aaataaaata aataaatcca aggaagattt aatcaggaaa tattatcaaa
     4441 aatataagat tggagaaagt tatgaatatg atttcagtaa taatgaattg atttttccgg
     4501 gagattttgc agtaaaaaac aaaataaata acttagtaca gggctctttt aataaagaga
     4561 gagcaattga agaaaatggt gttatattag ttagaaaaag tgcaattgaa gaaaatggga
     4621 agaaaggtgt agtagaactt accaactata aagtaggaga tacagtaaat tgtgaatatc
     4681 ttgatgaaaa tgccttaagt aaaaaagtaa aggtcaagat tcttgctata actgatgaag
     4741 aacgtctagg ttttggttat caaaatatgg ggttacaatt tataacttat gatgaagttg
     4801 ctaaaaattt aaatttaaaa ttaagtaaaa gccttatatt tattgattca ggaggaagca
     4861 ttaagactaa gaaggaaata gaagctttgg caaataaaaa tgattttaac ttttatgatg
     4921 aaatttcaag cagtgaaagt gaaaaacaag atttaaaggt tattaaaata tttg
//
LOCUS       DAEPLF010000230         4898 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEPLF010000230 DAEPLF010000000
VERSION     DAEPLF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.231"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..990
                     /locus_tag="KR314_003181"
     CDS             <1..990
                     /locus_tag="KR314_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBH0329541.1"
                     /translation="YDLKEKFFQSEIWNEFLDKININNNSKKDKKEQNQNLQSNNTEG
                     ENLGESDIKNFEKISLGDNLSYVLSSIGKPGRIDISEYGFDWYVYNQYGKEFAMVGLE
                     NDEVVALYSNSINSCENQDIKLNQDRQTVRTKITPLKYKRKGNTRYIINSENQYDIIS
                     KEGKYITIFYDIHEENRVCSYLIIDKSTEDEFKKLYPDDREELKKCFELEVIDLVNSV
                     RNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFDHINKKNETPFDRMKREGIVYTSAGE
                     NIAAGQINAIYAHEAWMNSEGHRKNILGNYNNIGVGVIFGGSYKTYYTQNFYK"
     gene            1018..1737
                     /locus_tag="KR314_003182"
     CDS             1018..1737
                     /locus_tag="KR314_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0329542.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVKNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            1761..2345
                     /locus_tag="KR314_003183"
     CDS             1761..2345
                     /locus_tag="KR314_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBH0329543.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGYVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIIDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLENFITEARERVHM"
     gene            2347..3696
                     /gene="pabB"
                     /locus_tag="KR314_003184"
     CDS             2347..3696
                     /gene="pabB"
                     /locus_tag="KR314_003184"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBH0329544.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            3686..4429
                     /locus_tag="KR314_003185"
     CDS             3686..4429
                     /locus_tag="KR314_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HBH0329545.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            4463..>4898
                     /locus_tag="KR314_003186"
     CDS             4463..>4898
                     /locus_tag="KR314_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329546.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISNIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKISNL"
BASE COUNT         2048 a          457 c          912 g         1481 t
ORIGIN      
        1 tatgatttaa aagaaaaatt ttttcaaagt gaaatatgga atgaattttt agacaaaata
       61 aatataaata ataattctaa gaaagataaa aaagaacaaa atcaaaattt acaatcaaat
      121 aatactgaag gggagaactt aggagagtca gatataaaga attttgaaaa aataagctta
      181 ggagataatt taagctatgt tttatcaagt attggtaaac ctggtagaat tgatattagt
      241 gaatatggtt ttgattggta tgtatataat caatatggaa aagaatttgc aatggtcgga
      301 cttgaaaatg atgaagtagt agcactttat agtaattcta ttaattcatg tgaaaatcaa
      361 gatataaaac taaatcaaga tagacaaaca gtgagaacta agattacacc attaaaatat
      421 aagagaaaag gaaataccag atatataata aactcagaaa atcaatatga tataataagt
      481 aaagaaggta agtatattac aattttctac gatattcatg aagaaaatag agtttgctca
      541 tatttaataa ttgataaaag tactgaagat gagtttaaaa aattatatcc agatgataga
      601 gaagaactta aaaaatgttt tgaacttgaa gttatagacc ttgtaaatag tgttagaaat
      661 caaagaggat taaactcatt gagatacagt gaacaggcaa ctttgagttc aagaaaacat
      721 agtgaagata tgagagataa taattttttt gaccatataa ataagaagaa tgaaactcca
      781 tttgacagaa tgaaaagaga aggtatagta tatacttcag ctggagaaaa catagcagca
      841 ggacagatta atgcaatata tgcgcatgaa gcttggatga actctgaagg acatagaaaa
      901 aatattttgg ggaactataa taatattgga gttggagtaa tatttggagg aagctataaa
      961 acatattaca ctcaaaattt ttataaataa ctaaaatatt tgaaaggaag ttaaaaaatg
     1021 acatcatgga aaaaaaagac agtatataag tgcttaatag cagtagtctt gttttgtgga
     1081 atagtgttaa tatctaattt tagtaaagtt tctgctttga tgatggatac taatggaaat
     1141 gttttgatta aacatggttc aagggaaaaa aagctaatag caattacctt tgatgatggt
     1201 cctcatccta aagaaactag tcaagtttta gatgtattaa agaaatacaa tgtaaaagct
     1261 acttttttta tagctggaaa acatgcaaaa tggtataaag aaccattagt aagagcaagt
     1321 aaagaaggtc acgagatagg aaatcatact ttcaatcatc ctgatataag taatttaagt
     1381 tctagtcaga tagaagagga gattgttaag tgtgaagaca ttttgaaaga agtaactgga
     1441 aaaaaaccta cattatttag accaccattt ggaagttata gagaaaaaga cttaatagaa
     1501 attgcaaaaa agcatgacta taaagttgtt ttatggacag gtgtagatgt gaaagattgg
     1561 aaaaatccag gagctaatag cattgcagat aaaataatta ataaagtaaa aaatggagat
     1621 ataatacttt tgcatgatta tgcaactaat gatacagttg aagctttaga tatgtttatt
     1681 ccaaaaatga ttgaaaaagg ttttaaattt gtaacagttt ctgagttgat aaaataattt
     1741 ttagggggga gatttacagt atgattctta tgatagacaa ttatgattct tttgtataca
     1801 acctagtcca atatatagaa gaattagggg aagcagttgt agtaaaaaga aataatgaaa
     1861 tcaaaataag tgacattgaa gaactaaatc cagaggtaat tgtgttatct ccaggaccgt
     1921 gttctccaaa agaagctggt atttgcatag atattgttga gcattttaag gggaaaaaac
     1981 ctatactagg aatttgttta ggtcaccaaa ctattggata tgtttttgga ggagatatta
     2041 taaaagcaca acagcctgtg catggaaaag tatatagtat caaccatact aataaaggag
     2101 tttttagggg acttaaaaat cctttaaatg tcactaggta tcattcacta ataattgatt
     2161 ccaatacagt tccaaaagaa ttagaaatta cagcaattac tgataaaggg gaaataatgg
     2221 ggattaggca taagaaatat ttaatagaag gagtacagtt tcatccagaa gctattttgt
     2281 cagagtatgg tcatgaaatg cttgaaaatt ttataacaga agctagagaa agggtacata
     2341 tgtaatatga taagagagat aaatactaaa ttaaattctt ttgagatatt tactattttt
     2401 agaaatgaac atgacagttt tatattagac agtgctatgg ataaagaaaa attgggaaga
     2461 tattctttta taagtagcca accatttaag gtattaaagt ataaggatac tgatgaaaat
     2521 cctttagaag ttttaaaaga agaactacat aagtataggg ttgtaaatga tactaatctt
     2581 ccatttgtag gaggtgctgt gggatattta tcttatgatt taggtaatta catagaaaat
     2641 ttacctagaa ctgctgtaga tgatattgag atgcctgata tgtattttgg attttataat
     2701 catgtaatag ttatagacca tcttgtgcaa aaaacttata tagctactcc aaacatagat
     2761 atcgaattag aagaaaagat aatagatgat atagaacaaa gaatattaaa agaagagaaa
     2821 aaaggtatag atagcatatg ttatgaagaa aaagaagtaa cacctataag acttaaatct
     2881 aattttacaa aggaagaatt taaaaatgca gttcaaagtg ttagagagta cataagacaa
     2941 ggagatatat atcaagctaa tttaacacaa aggttcagtg gagaaacaga acttacaagc
     3001 tttgaattgt atagggactt aagaagattt agcccagcac catttggggc attcttaaac
     3061 tttgaagata cacatatatt atcaaattct ccagaaagat ttataagatg tgttaataag
     3121 agaatagaaa ctagaccaat aaaaggtact cgtccaagag ggaaagataa agaagaagat
     3181 ttaagacttc aacaagaact tagaaatagt gaaaaagata gagctgaact acttatgata
     3241 gtagatttag aaagaaatga tattggcaga atatctaaaa ctggaagtgt taaagttcca
     3301 gagctatttg taattgaacc atatgcaaat gtaaatcact tagtttccac agttgtaggt
     3361 gagttaaaag atgataaaga tgctactgat gtaataaaag caacttttcc aggggggtct
     3421 ataacaggag cacctaagat tagagcaatg gaaattatag acgaattaga gccaacgcaa
     3481 agaaatgtat atactggttc aataggttat ataggtttta atggtgatat ggattttaat
     3541 atagcaatta gaacaataat caaaaatgac aaaaaagtct attttcaagt tggaggagga
     3601 atgacttggg attctgaccc agatgaagaa tatcaagaaa cacttgataa agctaaatcc
     3661 ataatgaagg ctttgagggg atattatgaa gaataatgta ggttttgata gtagtttaag
     3721 taaatttgga attggtttgt ttgaaactat tagagttaaa aatggaatag cagtagattt
     3781 agatatccat atagaaagaa tgctaagttc aataaattgt ttggatttag atataaacta
     3841 taaaaaagat ttcttgataa atgaaattgt tacatatata aaaaaagaaa atgtcattaa
     3901 taaagcactt agaattactg tttttgatga aggttataat atatctatta gggatattcc
     3961 atataatcaa gagacgtatg agaaggggtt taaattagct atatcgccaa tagttagggg
     4021 gaatagttta attcatagac ataagactac taattatttt gaaaacatat ataccaaaaa
     4081 tatcgcaaat aagactggtt ataatgatgg tatattttta aattcagaag gtgtaatatt
     4141 agaatgttct atgagcaata ttttctttat aaaaggagaa agagtgtaca ctcctagtga
     4201 tagattaccg atattaaatg gaataataaa aaaaaggatt atagagattt gtgacgaatt
     4261 acatatagag ttgatagaaa atgaaattaa tatatcagaa attagtagtt ttgattttgt
     4321 ttttgtcaca aatagtctta tgggcgctat aaaggttaca gaaattgaca aaataaaatt
     4381 taataaagaa aatgttgttt ttgataaaat agtgaagtta ttagaataaa ttaaaatatt
     4441 aacaatttgt tggagacaaa agatgatagg ttgtatttat gaagtataca atgaagagat
     4501 gactaaaata atagaaaacc aaaacacttt ggctgtatat ttagtaggtt ctagtaaaga
     4561 cgtagatttt actctttatg atgtagagat aaacgatatt gatgtgttcg tttttgtaaa
     4621 agaaggggaa aaacaagaga gaatactgtt taaaaaaaag ggaatagaat ttgatgtaaa
     4681 ttacttttca aaagatggtg taaaaaaatt attatcaaat agggaatatt tctttgtgaa
     4741 agaaatgaaa gatgctaaag tactgtttga tagggagaat atatcaaata ttataaaaga
     4801 tataagtaga gatatttact tagaaggacc cagtaaaatg tcattagagg aaaagtcttt
     4861 tataaagcaa gatattggag caaaaatatc gaatttaa
//
LOCUS       DAEPLF010000231         4854 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEPLF010000231 DAEPLF010000000
VERSION     DAEPLF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.232"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            185..1171
                     /locus_tag="KR314_003187"
     CDS             185..1171
                     /locus_tag="KR314_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0329547.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPMVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1394..1477
                     /locus_tag="KR314_003188"
                     /pseudo
     CDS             1394..1477
                     /locus_tag="KR314_003188"
                     /inference="COORDINATES: protein motif:HMM:NF012587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1508..2989
                     /locus_tag="KR314_003189"
     CDS             1508..2989
                     /locus_tag="KR314_003189"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBH0329548.1"
                     /translation="MYKEPKYRTILESIKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSVLEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3015..3986
                     /locus_tag="KR314_003190"
     CDS             3015..3986
                     /locus_tag="KR314_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBH0329549.1"
                     /translation="MKKVISIGEALIDFIPNQNGCKLKNVSEFRRVAGGAPANVSVVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFMTGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4242..4844)
                     /locus_tag="KR314_003191"
     CDS             complement(4242..4844)
                     /locus_tag="KR314_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0329550.1"
                     /translation="MEICILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEKHSRQFAKQIYYILDNSIEVDNC"
BASE COUNT         2060 a          501 c          791 g         1502 t
ORIGIN      
        1 aattaaatat aacaacaaaa aatgaaaatt acaattaatt taatgttaaa gactcaatta
       61 tttggatgtg gaatggatag tacaagtagt atataaaaag tattgactgg acaaataact
      121 atattttatt ttaaatatat aatataatgt aataaaatat atcaaatgta gaaggtgata
      181 acctatgaaa aaaataacaa taaatgatat tgctaacttg gcaggagtat ctaaaagtac
      241 tgtatcaaga tatctaaata ataaagatat cagtgattct actaaagaga aaataaagac
      301 aataatagat gaatatggat atgagccgaa tgcatttgct caaagtttga gagctaaaaa
      361 aacatacttt attggtatta taacaccgtg tttagattct tttgtaaaat ctaaaattat
      421 gatggcaata gatgaagaac tcaaagaatt aaaatataca tcactaatta ttaatactaa
      481 tagaaaaata aggtcagaaa tagatagtat atcaaagtta gctagtctaa aagttgatgg
      541 aataatacta attggtaccg aaataactaa agaacataag aatgaaattg aaaagttaga
      601 tataccaatg gttgtagttg gacaaaaagt tgatggtatc aatagcatag tgaatgatga
      661 ttatggagca ggatataaaa tgggtcaata tattgctaat aaaggttaca aaaacattgt
      721 atatttaggt gttgatgaaa gtgatatatc agttggttta aatagaaaaa atggagtact
      781 taatgggtta aaagataaag ggtatgatgc aaaagtattt tatactgact ttgaccaaga
      841 aacgtctata caaagaagtg gagaaatgct agaaagtgaa aatccagata taataatatg
      901 tgcaactgat aatatagcaa tagcaacaat gaaagaaatc aataagagag gtaaaaatat
      961 accacaagat atatctgtag ctggatttgg gggatatgat gtattgtcta ttataagccc
     1021 taaacttaca acaataaagt ttgaaaataa aaatgcaggt aaagtagcag caaatacgat
     1081 agtgaactta atacaagaaa gaaaagaacc actattaaag gagattaaat ttgaattaat
     1141 agaaggtgaa agtacaataa ataaaaatta agatacacat tacatatatt agtaatgtgt
     1201 atttttttac taaaaaaact ataattctaa aaattaggaa aacttttttg aaagaaatat
     1261 tgcatctgaa ataaatttat gctaatcttt aattaaaccg gtttcgaaac cggtttaatt
     1321 aaaccgaaag aatagcacaa tgagtgcaaa aataactgat tgtaacaaag tatcaaagta
     1381 attattagag tacataggaa ataaacaaaa tataaaaggt gtagcacatt gtgtaacaag
     1441 attaagaatt gttttagata gtaataataa agcaaatata aaattacaaa agaaaaggaa
     1501 taaaaatatg tacaaagagc ctaaatatag aaccatatta gaatcgataa aagaagaatt
     1561 aaataaatta agaaatatat ctttaaatga taaatataaa ccactatttc atatacatcc
     1621 acaacatgga ctacttaatg atccaaatgg attagcatat tataatggta agtaccatgt
     1681 attttatcaa tggtatccat atgatgcaac tcatggaatg aagcattggg cttatgtaag
     1741 ttctgatgat tttgtaaact ggaatagaga agatgtagca ttaataccaa tcgaaagtta
     1801 tgaatctcat ggagcatatt caggtaatgc gatagaggta gatggaaaat tgcatatgta
     1861 ttatacaggg aatataaaat atagtgcaga agatagatat gcatatcaaa acttagctat
     1921 aatgaacaag gatggtaaaa taactaaata tgaaaataat ccaatagtaa gtgaaatacc
     1981 aaaaggatat acaggacatg taagagaccc aaaagtattt aaaagaaagg ataaatactt
     2041 tatgttactt ggagcacaaa caagtgataa aaaaggagtt ataatagtat atgaatctaa
     2101 aaactctata gattggaatt ttaaaggaga attaaatgta aaaaatatag atgaagattt
     2161 tggttacatg tgggaatgtc cagattatat taatatagat gaaaaagata tattaatatt
     2221 ttcgccacaa ggagtagaac ctaaaggatt tgattatcaa aatatatata atgtagtata
     2281 tgctattggt aatatggatt tagataattt aacatttgaa atagatacaa tgaaagaact
     2341 ggaaaaagga tttgatttct atgctccaca aacatttata aaggactcac aaataatact
     2401 atttgcatgg gctggaatgg gagaagtttt ataccctaca gataaaaaca aatgggcaca
     2461 ttgtctaact gttccaagaa aattaaatat aaaaaataac aagctattgc aaatgcctgt
     2521 agatgaatta attaaattaa gatatgatga aacaagtggg caaaatacta taaagaataa
     2581 tatcaatatt atagaaaatg atgaaaatgt atatgagctt aatattaata taaaaaatat
     2641 tgacagcaat aaatttgggc tggaattatt ttcatcacaa gatgaaggtg taaaattaga
     2701 atttaacaaa ttaggaaaca ttgtaacttt agacagaagc aactttaaaa aagttttcgg
     2761 tgttgaatat ggaacaaata gaaaagaata tataaatatt gatgaaaata ctaatatcaa
     2821 agtactagcg gatagaagtg ttttagaaat ttttataaat gatggagaag tagtatttac
     2881 tagtagaata tttgctaaag aaaattctaa tcaaataagg gtttatagtg ataaaatagt
     2941 agagtatgag tatactaaat ttaaattaaa gcaaggtatt gaattataaa aacacattat
     3001 aaggggtaga aaagatgaaa aaagtaatta gtataggaga agctttaata gactttatac
     3061 ctaatcaaaa tggttgtaaa ttaaaaaatg tatcagaatt tagaagagta gcaggaggag
     3121 ctccagcaaa tgttagtgtt gttgtagcaa agcttggagg aaaatcaagt tttatatcta
     3181 agttaggaaa agatgcattt ggtgattata taatagatgt attaaatgaa gtaaatgtaa
     3241 atactgatta tgtattgaga acaagtaaag caaatacagg acttgctttt gtatcattaa
     3301 aagaagatgg taatcgtgat ttctcatttt atagaaatcc aagtgcagat atgcttttag
     3361 aagctgatga ggttaaaaaa gagtggttta ataattgtca tatcttacat ttttgttcag
     3421 tagatttaat agatagtcca atgaaattag cacataaaaa agcaatagaa tacgcacttg
     3481 aaagtaatag tataataagt tttgacccta atataagatt acctttatgg gatagtgagc
     3541 aatcttgtaa aaaggcaata tcagaatttt taccttttgc tcatattgtt aagatatctg
     3601 atgaagaatt agaatttatg acaggagaaa atgatataga aaaatcatta cataaactct
     3661 ttgtggggaa tgttgaacta gtattatata caaaaggaaa agatggagta gatgcataca
     3721 ctaaaaaagt aaaaggtatg tgtaaagggg ttaaagtaaa tgcaatagat acaaccggag
     3781 caggagattc ttatataggt tcatttttat acaccttatt gtataaacaa ataacattga
     3841 atgatataaa agaaatgaaa caagaaacgt taaatgagta tttagaattt tctaattatt
     3901 atgcagctcg tagtacaact attaaaggtg cgataagttc atatgcaaca aaggaagaga
     3961 taactgaatt tataaaaaat ttatagtagg aatgagtaat aggttttgaa ggaagtaaca
     4021 tgtaaagagt ttttaaggat gtttactatg aaaataatta acatataaaa agcaataaag
     4081 aggctgtctc aaaatagata taagcctctt tatcattgtt gaaatgataa aaaatatttt
     4141 aattattgtt actttttaga agaatatata tcttatttta aataagcaaa ttatgtaaga
     4201 tttatttata ttatcaatac tttacttgga gtattatgtc tttagcagtt atctacttca
     4261 attgaattat ctaaaatata ataaatttgt tttgcaaatt gacgagaatg tttttctttt
     4321 tctttattca taaaattagg tttacctaaa tcacgttcaa caagcattcc tatatatttt
     4381 aactgtgaat gcttacagta gcgcttcata ccattttccc aaatatcagc acccttttca
     4441 ggtctatatc cacatgttgt aataagtgct actggttttc ctgcccatag agcggggcct
     4501 ttctcattac catagtactt attcattcca tacaccaatc tgtcaagtag tgctttcatt
     4561 ggagaagtgc agtaccaaga ataaattgga gtagcaaaaa caattaaatc acacataaga
     4621 attttcttga aaattttctg aacatcatct ttacaataac atccaaaatc aaataagtta
     4681 tcctgacatt ttttacaagc aatacatggc tctattttac aatcatataa ccatattact
     4741 tcaatatttg aattataatt ttctaattcc tcaataaaag gttttaaaat agcagcagta
     4801 ttaccttttt tcttaggact tcccataaga atgcatattt ccatattgat acct
//
LOCUS       DAEPLF010000232         4790 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEPLF010000232 DAEPLF010000000
VERSION     DAEPLF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.233"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            34..486
                     /locus_tag="KR314_003192"
     CDS             34..486
                     /locus_tag="KR314_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0329551.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGIATNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            489..797
                     /locus_tag="KR314_003193"
     CDS             489..797
                     /locus_tag="KR314_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBH0329552.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            911..2017
                     /locus_tag="KR314_003194"
     CDS             911..2017
                     /locus_tag="KR314_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBH0329553.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            2092..4770
                     /gene="mngB"
                     /locus_tag="KR314_003195"
     CDS             2092..4770
                     /gene="mngB"
                     /locus_tag="KR314_003195"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBH0329554.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISNIENYIDSVKNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTANEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESSKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERSIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLLFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNNSKLKVNTKTVEVNSLSHCKVQTIVIK"
BASE COUNT         1959 a          577 c          834 g         1420 t
ORIGIN      
        1 agttaagaca ttacgaatgg agtggaaaaa tatatggtac tacaagaaaa atatatattt
       61 atagataaag acttaaaaag caaagaagaa gtattaaatt ttattgcaga tagtgcttat
      121 atactaggta ttgcaactaa taaagaggct cttttaaaag accttctaaa aagagaaaat
      181 gaatttccaa caggaataaa agatggattt gcgatacctc atacaaaaag tgtaaatgta
      241 aaggaagctt ccatactcta tattaaaaca actaacgagt tagagtggaa aactttagat
      301 gatagtaagg ttagatatgt atttagtttg ttagccccac ataaaaatga aggaaatata
      361 catttaaaaa ctctatctaa attagccaca tgtttaatgg aggatgattt tacaagtgaa
      421 gttaaaaatg ctagtgatgc aagtcattta gtaaaatata taactaataa aatgaaggag
      481 gaataattat gaaaatagta gcaataacat catgtatagc aggattagca cacacaccta
      541 tggcagcaaa agctttagaa caagctgcat caaaaatggg tcatgaaatc aaagtagaac
      601 aacaaggtgc tatgggacga gtaaatacta taacaaaaga agaagcacaa gatgcggact
      661 ttatacttat agcatctgac caaactatat ctggaatgga aagatttgat ggtaaaaaag
      721 tgataaaagt caaaataggt atagcgttaa aaaaaccaga agctgtctta acaaagtgta
      781 tagaagctat ttcttaataa aaaaatctca ctctaatata gaatgagaaa aataaaaaat
      841 taaaaaattg tctaatgtaa ttataacata ttatcaaaaa atataacaaa cttatatagg
      901 aggattaaaa atgagtaaaa aaaataaatc tcttttaagt gaacttaagg gacacttaat
      961 gacaggtatc tcatatatgt tgccacttgt aataggctca tctttagttg tagctatccc
     1021 aaagcttaca gctttagcaa tgggtataac aagtttagat gcttataaaa atgtatctgg
     1081 tttttatcat attctttact taatggaaca agttggttgg actggcatag gtctattaaa
     1141 tacggtatta gcaggattta tagcattttc aataggtgag aaaccagcta tgggtgcagg
     1201 ttttattggt gggctcatag caagtaatac taatgcagga ttcttaggag cagtaatagc
     1261 aggtttcttt gctgggtatg tttgtaagtt tttaaagaaa aaaattaaaa tatctgggtc
     1321 agcatcagga atgatgccac ttataataat gccacttata acagttggat taactggttt
     1381 tttaatgagt gttgtattag caggaccttt aggaaacata aatacttcat taaatgcttg
     1441 ggtagctgaa atgtgtcaaa atggaacaaa ttcggtggta ttggctttaa ttttaggcgc
     1501 aatgatagga ttcgatttag gtggaccagt aaataaagca gcttggatgg caggaaatgc
     1561 attattgtta gagggaatct atctaccagc gatattggta aactgcgcaa tagttatacc
     1621 tccactagga tatggtttag ctacattcat aagaaaatca aaattttcca atgcattagc
     1681 tgaaacagga aaaggcaata tagttatggg tataattgga ataacagaag gtgcaattcc
     1741 tttcacttta acaaatccat taaaattagt tcctgttaat atgataggat gcgctttggg
     1801 tgtaggatta tcagctttgt taggtgtaca tgctataatg cctccagttg gtggattata
     1861 tggatttata tcaataggtt ctggatgggc ttacttagta ggtgcaatat ttggagcttt
     1921 agtaatagca atattatcaa caactttagt agacttcaat gaagatgaaa ctacggaaaa
     1981 tacagaattt gatgaaatag agttagatat aatgtaaatt aaaaacttaa ttttagataa
     2041 ttgagaattg agtgtactct caattctcaa ttaaattttg ggaggatttt tatgaaaaag
     2101 acaaaagtac atgtagttcc acacagtcat tgggatagag aatggtattt tacaacatca
     2161 aggtcaaaaa tatatttaat gaaagatttt caagatatat tagacatatt ggaagaaaaa
     2221 gaggacttta aatactttac tatggatgca caagcttctc tcttagatga ttatttaaaa
     2281 tggagacctc aggatgaata tagaataaga gatttagttc aaagaagaag attagttatt
     2341 ggaccttggt atactcaaac agaccaattg gttatatcag gagaatctat aattagaaac
     2401 ctttactatg gaatagatag atgcaatgaa tttggtgaac ctatgagggt tggatatgta
     2461 ccagattctt ttgggcaatc agctcaaatg ccacaaatat acagaggttt tggaatagat
     2521 agttcattat tttggcgtgg agtttctgat gatatggtag atacaacaga gtttatatgg
     2581 aaaggttctg atggttcaaa agtattagca gttcaaatac catttggtta ttactatgga
     2641 ggtaatatac cagaagatga ttcagattta aaagaatatt taaaagatat aattggaaaa
     2701 cttaaggtta aagcttctac taataacgta tattttccaa atggatttga ccaagcacca
     2761 ataagaaaaa accttcctga tatattaaaa aaagctaatg aaatagataa tgaaaatgaa
     2821 tacgaaataa gtaatataga aaactatata gattcagtaa aaaatgaaag aaatgaattt
     2881 acagagctta aaggtgaatt tttaaatgct aaacatatga gaatacacaa atccatattc
     2941 tcctcaaggt cagaccttaa aataatgaat aacaaattag aaaattatat cacaaatgta
     3001 cttgaaccat tattaacatt aagttattca cttggaaatg aataccctca tctagttata
     3061 aaagatatat ggaaacttat gtttgaaaat gcagctcatg acagcatagg ttcttgtaat
     3121 agtgacacag caaatgagga tgtatacaca agatacaaac aagccagaga tttgagtata
     3181 aatttattag atttgcatat gagacttata agtactcaaa tcaaaaataa taatgaagaa
     3241 ataacattaa cagtattcaa tggattccca gaagtcagaa atgaagttgt agaatttgac
     3301 acttatattc caggtcaaga ttttgtgata aagaataaaa atggagaaat tcttaattat
     3361 ataataaaag aaaaagaaga tattacaaat tatatattaa ctcaaacaat aagattaaac
     3421 ccaagtaaaa aaatatatat acccaaaact gtatatagag caaaaattgc actttcagta
     3481 aaagatatac caccaatggg atatactcaa ctagtttttg aattggatac taaaaataaa
     3541 tgtgaaatag aaacaagtaa ttgcaaagaa atagaaaaca aatattacaa aataacagcc
     3601 aataaaaatg gttcattaga aatattagat aaagaatcta gtaagactta taaaaaccaa
     3661 gctgtaattg aagacaatgg agatgatgga gattcataca actattctcc tccaagaaaa
     3721 gatatttatg tttcatcttt agattctgta tcttcagtag aagtgctaaa atcaaatatt
     3781 caagaagaaa tgatacttaa atattcattg accataccag cttcactgga agaaaggtct
     3841 ataggtaaag tttctgtgaa aatgccagta acaatgaaaa ttaccttaga acctaaagaa
     3901 caaataatta agtttaatgt taatataaaa aataaccaag cactatctca tagagtaaga
     3961 gttttattta atacagaaat agcatctaag ttttctatag cagatcaaca atttggaata
     4021 atacaaagac caacagtatt agaaaaagac cttgcattat ggaaaagaga caattactca
     4081 tggcaagaaa aacctataac tatcgaacca atgcaaagtt ttgttacttt agaggatgga
     4141 caaagaggaa tagcattaat aactggttgt gtaagagaat atcaaatagt tggtgataat
     4201 ttagatacaa tagcattaac actattcagg tcatttggat atatgggaag agaaaattta
     4261 ctttatagac caggaagagc ttctggagaa aaaataatag caacaccaga tgcacaatta
     4321 cttaaagagt taaactttga ctttggtctt tacatatata acaataaatt tgatgaagca
     4381 aatgtagcta atacagctaa gagattctta acacctatac aaatatatga atatgctgat
     4441 tttttaaatg gtagacttct atttgcattc aatgacgaaa aacaaatata tgaaaatgaa
     4501 tttagtttga tgaatgtaaa taattcaaat ttcacagtaa gtgcaataaa gaaagaagaa
     4561 aaaggagatg gaattgtagt tagaatcttc aatggtatga aaaatgagga tgctaaggga
     4621 agtcttaaca tcaataaaaa tgtcaatgat gcttatagtg caatgctcga cgaaatctat
     4681 gataataata gcaagcttaa agtaaatact aaaactgtag aggtaaattc tttatcacat
     4741 tgtaaagtac aaacaatagt aattaaataa tataaaatag cttatacaag
//
LOCUS       DAEPLF010000233         4781 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEPLF010000233 DAEPLF010000000
VERSION     DAEPLF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.234"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..465
                     /locus_tag="KR314_003196"
     CDS             <1..465
                     /locus_tag="KR314_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0329555.1"
                     /translation="ELSRKTIDSLNRNKARLKVARLQEHIANQRKDFLQKLSTKLIKE
                     NDIICIEDLQVKNMIRNRKLSRLISDVSWSEFVRQLEYKANWYGRQIVKVGKFFASSQ
                     ICNKCGYKNEEVKDLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            616..1422
                     /locus_tag="KR314_003197"
     CDS             616..1422
                     /locus_tag="KR314_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0329556.1"
                     /translation="MGMILSTLGINLYLAPAKLLSGGVAGICVILYKLFGINQGISSF
                     LMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLTQDIAKYFPIDDTMLQCIYGGAL
                     TGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNTFLFINFFVVCAGAFLFGAKLVM
                     YTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEISKVIMNKMGRGVTFLEAEGAYT
                     QNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNNVEEVKGGGFKDKFL"
     gene            1549..1821
                     /locus_tag="KR314_003198"
     CDS             1549..1821
                     /locus_tag="KR314_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329557.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(1984..2556)
                     /locus_tag="KR314_003199"
     CDS             complement(1984..2556)
                     /locus_tag="KR314_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0329558.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            2740..3582
                     /locus_tag="KR314_003200"
     CDS             2740..3582
                     /locus_tag="KR314_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBH0329559.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            complement(3685..4761)
                     /locus_tag="KR314_003201"
     CDS             complement(3685..4761)
                     /locus_tag="KR314_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0329560.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
BASE COUNT         1757 a          616 c          770 g         1638 t
ORIGIN      
        1 gaattatcga gaaaaacaat cgatagttta aatagaaata aagcaagatt aaaagttgcg
       61 agacttcaag aacatatagc caatcaaaga aaggattttc tacaaaaact atctactaaa
      121 ttaataaaag agaatgatat tatttgcata gaagatttac aagtaaagaa tatgattaga
      181 aatcgtaaac tatctcgctt aatttctgat gtgtcatggt cagaatttgt tcgtcaatta
      241 gaatacaagg ctaattggta tggtagacaa attgtaaagg taggtaaatt ctttgcaagt
      301 tcacaaatat gcaataaatg tgggtataaa aacgaggaag ttaaggattt aaatataaga
      361 gaatggattt gtcctagttg taatgaaact cacgatagag atataaatgc aagcataaat
      421 atattaaaag aaggattaag actaataaca attcaaaata aataacttat gtaagaaccg
      481 taggaactac ggggatagct tgtggagaat tagtaagaca tattttagta tgcaaaattc
      541 tatgaagcag gaaccctgtg actttagtca tgggaggttc agaataacat atttttacaa
      601 tatggtctga ttatattggg tatgattcta tcaaccttag gaataaattt atatctagca
      661 ccagccaagt tattaagtgg aggagtagct ggaatttgtg ttatattata caaattattt
      721 ggtataaatc agggaatatc aagtttcttg atgaacatac caatatttat tatagctaga
      781 aagtattttg ataataaatt tttgctaata agttttgtca atatgttact gttttctcta
      841 gcactggggc taacacaaga tattgctaaa tatttcccaa tagatgatac tatgctacag
      901 tgtatttatg gtggtgcatt aactggtata ggtatggggt taacttttaa agcaagagct
      961 actgctggag gacttgatat aatagcagcc ataatgaaaa gaaagtatga tatacctatg
     1021 aaaaatacat ttttatttat taattttttt gtagtatgtg caggagcttt cttatttgga
     1081 gctaaattgg tgatgtacac tttaattaca atgtatataa tttcttttac tatggatata
     1141 ggaaaagatt gctttgatag aaaaaaatct atacttttaa tatcaaataa atatgatgaa
     1201 atatccaaag tgataatgaa taaaatgggt agaggagtca cttttttgga ggcagaaggt
     1261 gcatatacac aaaataagaa aaagatgatt tattgcataa tatctgcaaa tgaaataggt
     1321 aaatttaaag atttaattta tgatatggat agtgaagctt ttatttctgt caataacgtt
     1381 gaagaagtaa aaggtggagg ttttaaggat aaatttcttt aagattatat atgttaatta
     1441 tatatgttaa aaaatgccat atgtattgag attataccta gttagatgta tactgtagtt
     1501 aatggggtac atgaagtact caataatata ctaggaggta taagaattat gttaagagag
     1561 agatttgtag taagaaatca tcataatcca catacaagtg ggtgttgtgg gactggagga
     1621 gaaggatgct gtggaggaga taaccatgaa cattcacatc aacattctca cagtcatggg
     1681 catgaacatg agcatgtaaa tggtgtaggt cacgaccata atcatgatga tggtttttca
     1741 ggtactcatg agcatagcca tgagcataca catacacatg gtcatgaaca ttcagatggg
     1801 catacacatg aacataaata aaaacatttt aaatttataa gagattttta ttttgtgatt
     1861 ccatgaaggt ttaaataaaa attgaatgtt ttaagtaaaa attattgaga ataaataaga
     1921 aagtgaactg ttaaagtaga taacttaaat agttcacttt tattaattat ctaagtatta
     1981 agtttaaatc caatgattaa ctaaaaaaga agctatattt tcataaggta ttttttctgt
     2041 ttttaataaa ctttggacaa ctaaaccatc aactattgac atgagtaaaa aagaaaatga
     2101 ttcagcatcc tgttcatttt cgctactatt atatttttct agtgattcaa caatttcaga
     2161 tttaagagta gttctccatt caatatattt taatcttatt ctttccttta aatcattatt
     2221 tgaagtaatt gcctcacaaa taagatacat atgtacccta ccacgacttt ctatagtaga
     2281 tattttctct aagattaagt taaccatttc tatttgagaa ttttttgatt tgacattttt
     2341 tacacagttt agtatagcac tagttataac ttctaaatga gtatccgcaa tatcatatat
     2401 aatatcatct ttagatgcgt agtgataata caaagtacct ttacttatat ttacatcttt
     2461 tgcaatatca gcaaggctag tattttgaac tccatttttg gctattaaat tggttgcaga
     2521 tgataaaata gcctgtttca tattatttgt tttcataaaa cctacctttc catgtatatt
     2581 tataacaaat taataacatt ataagcttat gaatatttat tgtcaataaa gcgagaatct
     2641 atatctattg actttttttc attcaatata taaaataata attaaatcgg tcgtacgacc
     2701 gactgaataa ataaaataat atcttatagt gaggttaata tggataaaat aaaattgata
     2761 gtggacagtg catgtgactt acctgatgat ataatagaaa aatacaacat agaagttgtt
     2821 ggtttaaatg tatcttttgg agaagaaagt tatatatctg gaaaggaaat agataatgaa
     2881 actttctata gaaaaatgag tgagagtaaa gtactaccaa aaacatcatg tccttctcct
     2941 gataaatttt tagaagcata ccattgtcag gaagaaagtg tattggtgat taatataagt
     3001 tcaggattat ctgggactta taatagtgca cttttagcaa aagatatgtt tgaaaaggaa
     3061 ggaaataaca aaaaagttga ggttatagat tcacttagtg ggtcaattgg aataggacaa
     3121 ttagtgttaa aagcagcaat attaataaaa gaaggaaaag aaattgaaga gattgtttca
     3181 ataataaata aatataaaga taatcctcca ttttttggaa cacttgaaac tttagaaaat
     3241 gctattaagg gtggtagaat aaatccaata gcaggaaaaa ttataaatac cttgaatttt
     3301 aaggcgataa tacaagttgc agatggcgtt gtaactccta tagataaggc aagaggtgaa
     3361 ggaaacagtt tgaaaaagct gattacttta gtagagtcaa aaattaaaga taaagaagaa
     3421 aaggttttat ttataggaca tgctaattgc ccagaaaagg ctcaaaaggt aagggaagtt
     3481 atggaaaagg atgtaaaata caaagatgtt gtaatctgtg aaattggttc agtgatgggg
     3541 acatatactt caaagggtgc aatcttaata actgcaatat aaataatgga ttgaaataaa
     3601 tcatagattg agaaattaaa gccatataaa aatgtttaaa acatttttat atggcttttt
     3661 aattttatca aattgatttt agaattaacc aactacacat atttctttgt catgtttgtt
     3721 taatatctca caaccatctt cagtaacaag tactaaatct tctattctaa ctccgaactc
     3781 acctggtaaa tatatacctg gctctataga gaatatcatt cctggttgta cagtatctgt
     3841 atttatagaa gatacatctc ctttatcatg aacttcaagt cctatagagt gaccagttct
     3901 atgagtgaag tactgtccat atcccttttc agttatgtag tcacgagcag cagcatctat
     3961 atcacagaat ctaacacctg gttttacaat agcttcagct ctcttattag cttcaagaac
     4021 tatttcaaat atttctctac ctttttcagg tatttcttta tagaatacag ttcttgtcat
     4081 atcagcacaa tagttatctt ttatacaacc tatatctaat attattgcat ctcctggttt
     4141 cacaagtgca tttccaggtt caccatgtgg attagctcca ttaggtccaa aacctataat
     4201 agggtcaaat gaaaatccat cagtacctag gtcttcatat attttagata atttaccaac
     4261 aagttgtttc tctgtaagtt caccagaaac agtagcttta agttgttcca tagccttgtc
     4321 atttaatttt gaagctattc tcattaactc tttttcttct tcatctttac acattcttaa
     4381 agtatctatt atataagatg aatttacaaa cttacttcct ccacctaatt ctattaagtt
     4441 taataagaat ctagctggcc agtttttatc aacacccata gtagcatttt tgtctatatg
     4501 ttcagttatt atttctactg gattttgagt atcattaaac caaaccattt caacacctaa
     4561 atcttctgaa actgggaata attcatttat aaataattta ttattgccat ttaaatttaa
     4621 gtataatgct aataatcttt cacctggatg gattagtact cctgtaagat aaaatataga
     4681 agtagggtca cttactaaca tttgagaaat atcatctttt ttcatttgtt caagtacagc
     4741 atttaatctt tgagctttca taataatttc ctcctaatca a
//
LOCUS       DAEPLF010000234         4762 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEPLF010000234 DAEPLF010000000
VERSION     DAEPLF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.235"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(5..1063)
                     /locus_tag="KR314_003202"
     CDS             complement(5..1063)
                     /locus_tag="KR314_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBH0329561.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTIFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLEDGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINEIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            1130..1501
                     /locus_tag="KR314_003203"
     CDS             1130..1501
                     /locus_tag="KR314_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBH0329562.1"
                     /translation="MKKKDILLILGILVVIIACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            1538..2212
                     /locus_tag="KR314_003204"
     CDS             1538..2212
                     /locus_tag="KR314_003204"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBH0329563.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            2461..3300
                     /locus_tag="KR314_003205"
     CDS             2461..3300
                     /locus_tag="KR314_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBH0329564.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            3543..3639
                     /locus_tag="KR314_003206"
     tRNA            3543..3639
                     /locus_tag="KR314_003206"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3577..3579,aa:Sec,seq:tca)
     gene            3790..4497
                     /locus_tag="KR314_003207"
     CDS             3790..4497
                     /locus_tag="KR314_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBH0329565.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
BASE COUNT         1832 a          557 c          766 g         1607 t
ORIGIN      
        1 aaacttattg tttatttaat ttttcaatag aaaaagattt atctgtaagt ttaaaattat
       61 cttttatacc cttagttaaa tatactttct tatccttagt tatgaatata gcatctacat
      121 tatcaatttc attaattaaa tccatacctt tttcaagacc aagtccaaat gtagaagtgg
      181 ataaggcatc acaattaata gatttatcag aaattattgt aacactactt agattgtttt
      241 caaaaggata ccctgtaaat ggatttaaca tatgatgata tatttttccg tctttttcga
      301 taaatctttc ataaattcca gaagtaacaa cagatttatt tgtggtttct atgtttccta
      361 tggattcacc atttttacta gtggggtctt gaatgcctat tttaaaaggt ttatcatttt
      421 taccatcttc taaagtaaat atatttcctc ctaagtttac taaacctttt tttacgtcct
      481 ctgattttaa ataggctaca attttatcag cagcatatcc tttagctatg gcacctaaat
      541 caattttcat attttctttg gttaatttta tagatttatt tttttcatcc aatatgatgt
      601 tcttataatc aataagaggc agaagttttt gtatctcaga atcacttgga actttagctt
      661 tatctgttcc aatagcccat aaatttgata atggaccaat tgttatgtca aaatatccat
      721 ctgataattt ggaatattgt atagcttctt ttacgacgaa aaatgtatca tcagaaactc
      781 tgacaaattt tttaccagca ttatcattta ttttagaaac atcacttcct ggtatatgag
      841 tgctcatttt gttgtcaata tatcttaaaa ttgaatcaca ttcatttaag attttatcac
      901 ttttggattc ttttacatta aatatagtaa cttcgtttac tgtaccaagg tagtagctag
      961 ttttactata tttagtattc tggcttgaac tcctaaaaaa tgaaaataaa attaatgcta
     1021 aaatcaatat cacaaaaata aatgtgattt ttttcttatt catggtatat tcctcctatg
     1081 aaattgtgtt attattttat tataacataa tttcatagga ggacaaaaaa tgaagaaaaa
     1141 agacattctt cttattttag gcattttggt tgttattata gcatgttatg ggataataaa
     1201 tgttataaat tctaaaaatg ctggaaatat tgagatttat gtagataata aattgtataa
     1261 aactgtttca ataaatgcca aagaagaatt taaaatagag aatagagggg ggtataacat
     1321 agtaaagatt catgataaag gggttgagat agtagatgct tcatgccctg ataaggtttg
     1381 cgtacatact ggatttataa acaagccaag tcaaagcata gtttgtatac ctaatagagt
     1441 gagcataaaa ataaatacaa atgaaaagaa tgataatcaa gaagatataa tttctaaata
     1501 aaataataaa aataattgaa aggaattggt gataattatg gttaacttag gaaacgattg
     1561 ggatgaatta ttgaaagaag aatttgaaaa agattactac ttaaacttaa gaaaatttct
     1621 tatagaagaa tataaaacac gccaaatttt cccaaatatg cacaatatat atgaagcttt
     1681 aaaacacact tcatacaaag acacaaaagt cttaatatta ggacaagacc cttatcatgg
     1741 agataatcaa gctcatggtt tagctttttc agttcaacca caagtgaaaa ctccaccatc
     1801 tctattaaat atgtataaag aattaaaaga tgatttaggt tgttttatac ctaataatgg
     1861 atatttaatg ccttgggcag accaaggagt gttactttta aatactgctc ttactgttag
     1921 agcgcatgaa gctaattctc ataagaacaa aggatgggaa attttcactg atagagtaat
     1981 atctatttta agtgagcgtg aagacccagt tatttttgta ctatggggaa gtaatgcacg
     2041 taaaaaagta gagttgattg atacaagtaa acattatatt ttggaagcac ctcatccaag
     2101 cccattgtca gcaagtaaag gcttttttgg atgtaaacac ttctctaaga taaatgaaat
     2161 tttgaaaaag ctaggaaaag aacctattaa ttggcagata gaaaatatat aaaagtaaaa
     2221 ggtaacaaaa tgataacaaa acaacaataa acaatgacat tgttgtaact aatagaaata
     2281 tgcttaaata gttgaaaata ctatgtatgc atatttctat ttttttgtca agttgacaac
     2341 tcttgaaaaa atagttacaa tttagttaca caaaaaacac aaataaaaaa agaaacttac
     2401 aaagcagaat tttatgaagc aaaatatata gtgtaaaaaa ttttagggag gaaattttat
     2461 atgaatttta aaaaattgag taatgctaaa gtctcggtaa tggcagttgt tttatcagtt
     2521 ggaatattat cgggatactc tgcgcttaat aaacaagtaa cattagtagt aaggggagaa
     2581 gaaagaacta tatcaacatt tagttctaat gtaggagacc ttttagaatc tcaaaacatt
     2641 agctatgatg caaatgatat agtaacttta gatgaaaata caaagttatc taatggagat
     2701 aaaatagaag taattgatgt aaaggaaaaa acagttgtag aaactaaaga cattccattt
     2761 gaagttacag ttgttgaaga tggctcttta ttaaagggtg attctaaggt taaagaagaa
     2821 ggaaagtctg gtaaaagtga attaatatat aaagaaactt atcacaatgg aaagaaagtt
     2881 gaaaaggaat ttgtgaaaaa ggttgttaaa gctgagccag taaataaggt tgtaagcaaa
     2941 gggacaaagg tagaaatgca agtagcctca tcaaggggag aaagttcaag aagaatggct
     3001 tcatcatcat ctagtagcag tagtagtaac aatggtaaaa atatgagagt ggtagcaact
     3061 gcatatactg gtcatagtat aactgcaaca ggaacaaaac ctaagtgggg aacaatagct
     3121 gtagacccta aggttatacc atatggaact aaagtttata tacctcaatt tgacatggtg
     3181 tttactgcag aagactgtgg tggagcaata aaaggaaata aaattgatat ctacatgaat
     3241 gattcaaaaa cggtttataa ctggggaaga aaaactatag atatacgtat attaaaatag
     3301 ttaaataatt tatattttta ctggaaatta tattttgatt atatagagaa gaaactcttt
     3361 atagattctt aaaatataat ttccagtttt tattttggat atgtaataaa acaggtaaat
     3421 tttaaccatg tatagataaa agctatttaa attataattt actatcagat aaatctataa
     3481 aaattgttgc aaagagtcaa aatatatgat attatagatt tcgttgagaa ggatttcatt
     3541 atgggagtgg atgggtgccg gtgtgccctc tagtcttcaa aactagtatg aggggttaag
     3601 agcctcttag gtgggttcga ttcccacgca ttcccgccaa tatgcaaaag gtatctttta
     3661 attaaaagat acctttttaa atttatttta agataattta tttttaataa tatataatgt
     3721 tttgtattat ataatgtaaa ttttctaata ctattatata tttagataaa aatctaggag
     3781 tgtggaaaga tggaaaataa ttttacaacg agaactagtt ttctagttgg agatgatggg
     3841 atagaaaaac ttaataattc aaatataata gtatttggag ttggaggagt tggtagtttt
     3901 actgttgaag ctttagcaag agctggagta ggcaatatta ctattgtaga ttttgatgat
     3961 gtagatataa ccaatataaa tagacaaata ccagccctac attcaactgt aggaaggtac
     4021 aaagttgatg tcatggaaga aagaatatta gatataaatc ctaatatcaa tataaaaaaa
     4081 ataagaagtc tttataataa agatactagt gacgaaatac taactgaaag atatgattat
     4141 gttgtggatg cgatagacat ggtaagttcc aaaatacatc taattgaaac ttgtgaaaaa
     4201 aaagggttga aaataataag ttctatgggt atgggaaata aattagaccc aactaaaata
     4261 gtcgttacag atatacacaa aacaagtact tgtcctctag ctaaagttat gaggaaagaa
     4321 cttagggata gaggtatcaa aaagttaaaa gtagtatatt ctactgaaca accaatagaa
     4381 cttaagaaga aagtaatgaa tggtagaaaa gtaacacctg gtagtgtatc atttgtccca
     4441 tctgtaggag gtttaataat cgcatctgta atagtaaatg aactattagg acaataagtt
     4501 ttaaatattt taatttataa attttatata caaatttgaa ggctagaagc atgaatataa
     4561 agcaatttta tattcatact tttagcctaa ttattttata ttaccttatt ttatgtagtt
     4621 gtagttttat tttttagatt atgtatctat ttcataatta tattttgtaa atataatttc
     4681 tccatcacta taaaaaacaa tatctcctat tctatctgtt ctataaattg atacatggtt
     4741 ttgttttaga ttattaataa cc
//
LOCUS       DAEPLF010000235         4704 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEPLF010000235 DAEPLF010000000
VERSION     DAEPLF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.236"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            299..1159
                     /locus_tag="KR314_003208"
     CDS             299..1159
                     /locus_tag="KR314_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0329566.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1149..3020
                     /locus_tag="KR314_003209"
     CDS             1149..3020
                     /locus_tag="KR314_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0329567.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRLTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEASNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3040..4494
                     /locus_tag="KR314_003210"
     CDS             3040..4494
                     /locus_tag="KR314_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBH0329568.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLMDLYLKYSETVI
                     RRFDDKVKYWITFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
BASE COUNT         1801 a          499 c          878 g         1526 t
ORIGIN      
        1 gtaaatatta gtgacagtaa aatagtatca atttagtata taaactttgc cagataatcg
       61 cataacagag cttgtagtta tagaataaaa aaatatctag gaaaccgttg acagatttat
      121 atggatagta taacatatag aatatgaaat taaacaaaaa agaattttaa ttatggcgac
      181 gtagtcataa ttttgagtat gttactgatt agcaggcaga actcaagtat atatgtgaaa
      241 aatagaggga tactctatac atatatgctt gagttctttt tattgaaagg aggaatatat
      301 gaaaataatt cagattttaa ataataatgt agccttagta aaaagaggta aaaatgaaat
      361 tattgtattt gttaaaggaa ttggctttaa aaaaagagtg ggacaagtaa taaatgaaaa
      421 tgaaatagag agaacatata tactagattc atacgatatg ctagaacact ttagttatct
      481 actacaaaac tcagactcca ataatatagt tcttattaat gaaataattg aatatggtga
      541 gaaagcttta aatataaagt caagtgatta tctaagctta accttactag accatttaga
      601 gtttttactt aaaagatgtg aaaagaatca atttataaaa agtcctttgg tatggaatgt
      661 taaaagattt tatccaaagc attttgaaat tggaatgcat gcactaaaat taattggtga
      721 tagtaaggga attaatcttc ctgatgatga ggcagtatca atagcacttc attttgtaaa
      781 tatggaggta aataaagagt ctcatgatag tacaattgta gagttgagaa ctttagcaga
      841 tatagtttct ataataaaat atcattttaa tgtagagcta gatgaaactt caacaaatta
      901 tatgagattt actactcatc tacagtactt tatccaaaga attatgtcag atgaagcttc
      961 aatagatgaa gataattcta tagatttata taaacaagta agtcaacttt atccaaagtc
     1021 atttggaagt gttcaaaaga taagaaaata tataaaatca caatttaatg tcgatatatc
     1081 tataagtgaa gaaacttatt taatgttgca tataaatagg gtgactcaaa gactggaggc
     1141 aaaatcagat gaaatataaa gatttaaatg aaaatataat aaaacttata gggggaaaag
     1201 aaaatataaa gtcggtagcg cattgtgtta ctcgtttaag acttacttta aatgatagaa
     1261 gtatcgctca aacagaagaa attaaaaaat taaaaggagt aattgatgta gtttctaatg
     1321 atgttgctta tcaaattata ataggtactc atgttgtaga tgtatataac gagtttatgt
     1381 ctatgatagg tttaagttca agtgaatcaa gtataaaaag tgaaagaaaa aagggaatta
     1441 gaggtatcaa aggtatattt aattccatct ttgtggtagt ctcagagact atgacttcag
     1501 taattgaagt actacttgca gctggtatac tcgcaagtat attagcactt ttaaatatgt
     1561 ctggcatact tcagagtgat agccctacat atcaaatatt agatacatta agaagtgcta
     1621 catttcactt cttaccagta ttaattgcaa tagcatcagc aaaaagactt aatgtaaatc
     1681 cctatatagc agtagtgtta gcagttacgt tattatcgtc atcaatagat ggtgtagaag
     1741 gattaagttt atttggtata aatttacagg ctacaactta tgcaaacagt tttattccaa
     1801 tacttttagg agtatgggca atgggaatta taattgaagg tttgaaaaaa atattaccta
     1861 aagcactaca ctatttttta gttccagtgt tatctttaat aataacccta tcagttacat
     1921 taactgtatt tggacctatt ggtatgtgga ttggtgatgg attaaactat gcatgtatgt
     1981 ttttaattga tacattgggt aattggagtg ttgttgcact gtatgcagct ttacaaccac
     2041 tcttaatagt ttttggtgca gcaaacttta ctatgcctat atctttaaat tctgtgacaa
     2101 cattggggta tgacccaatt tttatgggcg ctgcaacaat atctgatatt gcagtttgtg
     2161 gtgcaatgtt tggatatttc ttaaaaacta ggaataaaga acaaaaacaa ttatttggga
     2221 cagtaagttt tagtgcactt atgggagtta cagagccttc agtgtttggt gtgtttatga
     2281 aatatagaag accatttgta gcagtaatga taggtggagg tataggtggt ttaatagcag
     2341 gtttagctca agttaaaaca tatggaatgg tttggggatt agctgcatta ccaacatatc
     2401 ttgttggagg agaggcatct aactttacat ttatgataat aagtgtagta gtatcatttg
     2461 tagcagcaac agcagcttcc tatttactag gaataccttc agatgaaaaa gaagaagtta
     2521 agggtgataa tgaaaaagaa ttagaactaa taatcaacga gaacaattca agaactatta
     2581 gtattggaaa tgttgcaaaa ggtcaggtaa ttgcacttgg ggaagttaat gacaaagcat
     2641 tttcctctgg agcattaggt aaaggggttg gagttatacc tagtgaatca aaatcaactg
     2701 ttatatctcc tgtagatgga actataacta tagtatttcc aactaaacat gcatatggta
     2761 tagtgactga tgaaggatta gaaatattaa ttcatattgg tatagatact gtaaacttag
     2821 atggaaaatt ctttgaaagt cttgtaacac aaaatcaaag ggttaaaaaa ggagatgctt
     2881 tagcagtatt tgaatctgat aaaattatag aggaaggctt tgattctact ataataactg
     2941 tagttacaaa tactagcgat tatttagatg taatctcatc aaatgatgaa tcagaagaat
     3001 tattaactgt aatagtataa attttaggag ggaaaattca tgaatattaa agatacattt
     3061 ttctggggag gaagtattgc agcccatcaa tgtgaaggtt catgggatag tgataacaaa
     3121 ggacctgcaa ttatggattt tgtaactaag ggctcttatg aaacgccaag agtaattact
     3181 gataaaatag aagaaaaatt ggattatcca tctcataatg gaatagattt ttataataga
     3241 tataaagaag atatagcttt atttaaagaa atgggattta gtgcattaag aatatctata
     3301 gattggtcaa gaatttttcc aaatggagat gatgaaaatc ctaatgaatt agggattaaa
     3361 tactatgaag gtctgataga tacattaata gaaaataata tagaacctat tgtaacacta
     3421 tatcattttg aattacctat gaatttagta cataaatatg gttcatggaa taacagaaaa
     3481 cttatggatt tgtatttaaa gtacagtgag actgtaataa gaaggtttga tgataaagta
     3541 aagtactgga ttacatttaa tgagatgaac catattgacc ctcaaacaga gcactcggat
     3601 atttttactt atatgatagc aggtattaag tatagtgaaa tagaaaataa ggttcaaaca
     3661 ttggcaaatg tagggtataa tatgactttg gcgggtgtaa aggttgcaaa gttggctaga
     3721 gaaatcaatc ccaacaataa agttggatgt gtatttggac taaatccaat ttatccatac
     3781 aattgtaatc ctagtaatgt gttaaatgca tttttagata atgatcgtga ttattatcaa
     3841 attgatgcaa tgtgcaatgg gaagtttccc caatataaat taaaagaata tgaaaaaaat
     3901 aatataacta ttgaagtgac tgatgaagac aaaaaagcat ttaaagaagg taagttagat
     3961 tttataggtc ttaattatta tttttcaagt gtatctactc caaaagaaaa tttatcaggt
     4021 gataaatctt tatttggtgg gattcaaaat ccatatttgg aacaaagtaa gtggggatgg
     4081 gcaatagacc ctataggttt aagatttacc ttgaactata tttacagaaa gtatcaatta
     4141 cctatcttaa ttacagaaaa tggtttaggg gcatatgata aaatcgaaga agatggaact
     4201 ataaatgatg attatagaat tgaatattta agtaagcatc tatctcaaat gaagtctgca
     4261 attgaagagg ataatgttga atgttttgga tatttgatgt ggggaccaat tgatttagtc
     4321 agtgcaacta caggtcagat gagtaagaga tatggattta tatatgttga cttagatgat
     4381 gaaggcaaag gaagttttaa gagatataag aaaaaatcat ttgattggta caaagaagta
     4441 ataaaatcta atggagaagt tttagatttt tctacggata tagtagtagg gtagtaataa
     4501 gataaataga agttaggaac taatgttaat tagtgtctag taaagcattt aaaatagagt
     4561 atagggtctt taaagtaaag tgtgtattct tattaaaagt aatggcaact aaaattacta
     4621 taaaaaggag aatacacatt ttttatgttt atacttaagt aaatatttag acatattcat
     4681 agtataattt actcattata taaa
//
LOCUS       DAEPLF010000236         4663 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEPLF010000236 DAEPLF010000000
VERSION     DAEPLF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.237"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..1490
                     /locus_tag="KR314_003211"
     CDS             <1..1490
                     /locus_tag="KR314_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329569.1"
                     /translation="NFEKHIETMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITI
                     LSENLISIIRESVSTLSLIVVLSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNR
                     TWKERKAWYIDYLLSKGYAYKEIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIAT
                     LLCTVLDSIVSAGIIGFIIIQGILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQ
                     DLFYTDLLFKFLDYNCKEDSIEHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINL
                     TLNKNDPIAILGKNGSGKTTLLKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCM
                     FQDYIKYEATLKENVAMGDVYKSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGK
                     KQLSMGEWQKVAISRTLMKDADMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFIT
                     HHIQHAKKITNNIVVLENGKLLESGRHDELMDKKGVYFNLFCNCK"
     gene            1512..2933
                     /gene="ccpM"
                     /locus_tag="KR314_003212"
     CDS             1512..2933
                     /gene="ccpM"
                     /locus_tag="KR314_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HBH0329570.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVNKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            2962..4032
                     /locus_tag="KR314_003213"
     CDS             2962..4032
                     /locus_tag="KR314_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HBH0329571.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            4048..4299
                     /locus_tag="KR314_003214"
     CDS             4048..4299
                     /locus_tag="KR314_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HBH0329572.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            4350..4553
                     /locus_tag="KR314_003215"
     CDS             4350..4553
                     /locus_tag="KR314_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329573.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
BASE COUNT         1911 a          419 c          765 g         1568 t
ORIGIN      
        1 aaaattttga aaaacatatt gaaacaatga tgttagagaa atctttaact ttgagtttaa
       61 aagattttga ggatgataat acttatgaca tgataaatag agctcaatct caaaaaggat
      121 ttaatattac tatactatct gaaaacctta tttcaataat aagagaatca gtatctacac
      181 tttctcttat tgttgtatta tcacaatttc aactttggct aataatagtt gttattgtta
      241 ttcctgtatt gaaatcatta tattccatta aattaggaaa ggaacaatat gatgttagtg
      301 taaacagaac ttggaaagaa agaaaggcct ggtacataga ttatttgttg tcaaaaggtt
      361 atgcttacaa agagattatg gtatttagaa ttggttctaa acttattaat cattataaaa
      421 aattaaaaga ggatattata tctcaagata tgaatatagc taaaaagaat tttattgcta
      481 cgctattatg tacagtttta gattcaattg tatctgcagg aattatagga tttataataa
      541 ttcaaggtat tttaaagaag attttaatag gagatgcgac tacatatata caagcagttc
      601 aatctataaa ggataatgtt gaatcaatat tttcgttatt tagttcagta atacaagatc
      661 ttttttatac agatttactg tttaaatttc tagattataa ctgtaaagaa gactcaattg
      721 aacatataaa aatatttgaa aaaatagggg atataaataa aatagagtta ataaatattt
      781 catataaata caatcattca tcagagtatt ctttaaaaaa tattaattta actttaaata
      841 aaaatgatcc tattgctata ctaggaaaaa atggttcagg taaaacgaca cttttaaaaa
      901 tattattagg tttttatgat gattatgaag gtgacatctt gataaatgga aaaaatttaa
      961 aggatattga aaaagatgaa tacattagaa aagtaagttg tatgtttcaa gattatatta
     1021 aatatgaggc tacattaaaa gaaaatgtcg ccatgggaga tgtatataag agtaacttgg
     1081 aagaaaaagt taaaagtaaa cttaaagaaa ctaaattgaa accaagtatt tatgaggaag
     1141 atggattaga aactatttta ggaacctggt ttggtaagaa gcaactttct atgggtgaat
     1201 ggcaaaaagt agcaatatct agaactctta tgaaagatgc tgatatgtac atttttgatg
     1261 aaccagattc atctttagat tctgaagcgg gaaatgaact cctttcttta tataaaaaaa
     1321 catttgaaaa taagattgga atatttataa cgcaccatat ccaacatgca aaaaagataa
     1381 ctaacaatat tgtagttctt gaaaatggaa agttgttaga aagtggaaga catgatgaac
     1441 ttatggacaa aaagggtgtt tattttaatt tattttgtaa ctgcaaataa atattaaagg
     1501 gaggttaatt aatgaatcaa aaaccattta tacacacatt taaagctgga aaatcatatt
     1561 ttatttatga tgttaatacg gataagattt taaaggtaaa tgctgctgtt tataattatc
     1621 taaataaaat tgagcataat ttagaaaaag aaagtgactg gaacatagaa atagaagacg
     1681 aaattaatac cttgattaat tatggatttc taaaaaataa aagggtaaat aaaacaatgc
     1741 accctgaaac taagtatata aaatatcata tagaaaataa tctatcctct attattttac
     1801 aagtaaccca aaattgtaat ttacgttgtg actattgcat ttattctggt tcatacagca
     1861 acagagttca tacaaataaa agaatgaata tagatgtagc aaaaagaagt attgactatt
     1921 tgattaagaa ttctagtcaa ttaaatgaag ttagtgttgg cttttatgga ggagaaccct
     1981 tacttgagtt tgatttgatt aagcagatag tagaatatgt aaaagtagtt ggagaaggaa
     2041 aggaaatata ctttaatcta actacaaatg caactttatt taatgaggaa ataatagaat
     2101 tctttgtaaa aaataatata acaactatga ttagtttaga tggaggaaaa gaaattcatg
     2161 ataaaagtag aaaatttgca aatacaggag aaggatctca tgatgttgta ttaaaaaatg
     2221 tacgttacat aaaaaataaa tatccagaat attataaaaa gaatattaca tttaatacag
     2281 tgataaattc agaaaatgat tacatttcta tagatgattt tatatctaaa aatgagactt
     2341 taaaagattc aaacttttta gcttccactc ttaataataa ctattctgat aaagaaatag
     2401 tttcatcaga tgaatatata ttaggtaaga gttatgatag attcttgact ttactcaaca
     2461 aattaaaatt tataaaagat gagagtatgt cacctttatc aagaatagaa agagccttat
     2521 tagatgattt tagatatgag aaaaatattg aaggtagatt agagttacca gatgtgtggc
     2581 atcatggtgg accatgtata ccaggggctt ttagaatatt tgtaactaca gatggagaat
     2641 tttttccatg tgagagagta agtgaaagtt ctaaaatggg tatgattgga aatttagata
     2701 gtggagtaga tatagaaaag gctgaaaaac ttttaaatat aggaaaatta tatgaagagc
     2761 aatgttctga ttgttgggcc tatagttatt gtgatatatg tatagctaag attgatgata
     2821 ctaaatgtaa tgaaaatgaa ataaaaccac atgattgtga cagaattaga cattctacag
     2881 aaaataatat gaaagattac tgcgttttga aatctctagg atatcaatat taaattttct
     2941 aatgaaagga aagatgggat tatggaaaaa gtacttatat atccatttaa ttataatttt
     3001 tttactattt taaatcattg caataaaatt tcagatatag aaattaattc actggttagt
     3061 ccttctggct gggggttatg caataaggag tataatgtta ataatattat tactaaagtt
     3121 acaaatgatt ttcaaagtga acttaaaaag aattctttgg tatggtttgt tgattcaaaa
     3181 gaaaatctag acttagaaaa atttataatt cctaaattaa aagtagctct aaaagaaggt
     3241 aaaaagatat tatatacacg tgatgatctt aataaaatat ctaggtatat atctcattat
     3301 gaattcaaaa agattaatga atggaattta gaaaaatatg aaataagaga tatagagggt
     3361 atatcacaga taaacacacc aataatattt atttgtggtc tttatggagg gttagatact
     3421 tttgatgtaa atataaggat aggagaagaa ctaagaaaaa gggggttttc aatattacaa
     3481 tttggtccta aaaaagagag ctatttattt aaaatgattc ctataccaga gtttatgttt
     3541 gatgattcga taagtagtaa gacaaagatt attaatttta accattttat aaaagataaa
     3601 gaggtagagg caaagccaga tttaataatt atagaagttc caggagaatt attccctttc
     3661 tctagaaaaa tggttggtaa ttttggaatt agagcttttg aaatttctaa tgcagtaata
     3721 aatgattgtg gtatattatg tattccatca agtatatatg atagagaata ttgtgaagaa
     3781 cttaaatatt ttattaaagg aagacatggt ataaatatag attattttag tatagagaaa
     3841 aaagttatag atacatatga gacagaggtg aagcaaaaat tttcatatat gtcattagat
     3901 gaaaactttt ttgttgaaga tatagaagat tatgaaaaaa cattttttat acatgataat
     3961 aaacaattac tagaattagt ggacaatata gtgaatcaat taaaagagta tgctaaagta
     4021 atatctattt agaattggag gttaattatg aattacactg aaaaggaatt actactagag
     4081 aatatatttt ttgatgtgac tggaattaat tttaataaaa ataacaattt aaaaaaagaa
     4141 ttattttttt caccgtcttt acatatacag ccaagagaat tacttttagt attttgcgag
     4201 gtaatagaaa actttaatat aaaaattcca gaggaaaaac ttttgaatag gcaatttacg
     4261 agttttgata atgttctaaa aataatcaat atggtatagg ttaataaagg tataaagtaa
     4321 gtgatcttat agaaaagggg aggatatgaa tgagttttgg aaaaagaata gaaatgaata
     4381 atataggtgt tgagacaaga ggtgtttgta ctattatgtg tccttatact tgtaagatga
     4441 tggcatcttc atcttgtaac tgcaagaata atgattataa tgcaagagga ggatcatata
     4501 atacatatta taataaattg atacatggtc aaagaactcc tgatggaatg taaatatttc
     4561 agtaataagt atactattaa attgatttag catatttgat taaatttatc tttcttactt
     4621 tatttccttt tattataaaa atatgcagtt gattataatt aag
//
LOCUS       DAEPLF010000237         4662 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEPLF010000237 DAEPLF010000000
VERSION     DAEPLF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.238"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(10..546)
                     /locus_tag="KR314_003216"
     CDS             complement(10..546)
                     /locus_tag="KR314_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBH0329574.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(659..1333)
                     /locus_tag="KR314_003217"
     CDS             complement(659..1333)
                     /locus_tag="KR314_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBH0329575.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            1536..3410
                     /gene="asnB"
                     /locus_tag="KR314_003218"
     CDS             1536..3410
                     /gene="asnB"
                     /locus_tag="KR314_003218"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBH0329576.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEYVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(3502..>4662)
                     /locus_tag="KR314_003219"
     CDS             complement(3502..>4662)
                     /locus_tag="KR314_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBH0329577.1"
                     /translation="KIFNIYDSSKFNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDL
                     FSTSMPNCTSSIIKNFAVKLLCWMDYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFL
                     YFLSQLGCDILYINPNKDILDLYPECSKFSTLFELSRKTPNILEIPFERHITNINYSE
                     NTNKTSPTTNKPNSKNTLNNTNLTSKKIAQDEEVELSYEDLAKLSASVVMIVVCNNEN
                     KPFKSGSGVVINNEGYILTNLHVVNDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVI
                     KVDRKCKPIPVKVSKKPVRGQKIVAIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAP
                     ISSGSSGGALLDMFGNLLGLISAGYDDGQNLNLAVESNLVKIFANNFIEIVN"
BASE COUNT         1814 a          563 c          680 g         1605 t
ORIGIN      
        1 tttctacaac tataataaaa tataaaattg atatccaatc aaaaaaataa ctatgaaatt
       61 taatagctta gatatgaatt tttctgtttt tgagttagtt tttataggta gtaaagaaat
      121 gtttatggta atgggataaa acaattcaat accttcttta gtaataaggt caagacacaa
      181 gtgagataga tatcctatta taaacccact ggacaaacat gagaaaacta tattattgtt
      241 agtgaagatt gtcagagatt taaaaaaact ataaataagt aataaggcaa ttagactgtg
      301 agtaaaccct ctatgtctaa acctacttcc aaataatttg tatattaaca taaatttttt
      361 acttatgtaa gaacctctca tatctatatc tggaaataaa gaacctaaat atgcaaaata
      421 aatataaatt ataattaata ctattttata tgcaatatcg tatttaacta ggtacagatt
      481 gtttataaag gggagtatca aaagtgcgaa tatataacct cccctttcat gtgtttcttt
      541 tatcatataa aaatcctcct gaatcgtcac tataaacatt atattggtaa atttagacta
      601 tcttttatta tattatatag caaacataag tttttttcaa aaggatttta atatttaatt
      661 aatcttgaga ttttaaattt ttgtaatttt ctaaagcacc attccaattt actacattaa
      721 accagttgtc aatgtattca tttcttctat tttgatattt taaatagtaa gcatgctccc
      781 aaacatcaag tcctattata ggagttaggt ttttacttac agggctatcc tgatttggag
      841 tagtcataat agataatttc ccatctttag tagctacaag ccaagcccaa ccagaaccaa
      901 agacatctaa agcagatttt tggaactctt gcttaaattt ttcaaaagaa ccaaagtctc
      961 tatcaatagc ttcttttaaa gattcagaag gtatggtttt ttctggcgtc attatatcaa
     1021 aaaagaattt atgattataa gctccacctg cattatttct tacagttgta gcaatatctt
     1081 taggtaaaga atctaaattt tgcaataatt cacataaaga ataattataa agctcaggat
     1141 atttttcaag agcagcattt aatttatcaa cataagcttg ataatgctta tcatgatgca
     1201 gtttcattgt ttctttatct atataaggtt caagtgcatc atatgcataa ggtaatggtt
     1261 taaccttaaa tttgttattt tcaggtgtaa aagcaaatga agttatgcac tgtgagatta
     1321 taaataaaga cataataact ggtattaata tttttttctt cataaaaatc acctcctaat
     1381 atagtatttc tagaaataaa aaaaatatga aatattaaca gataatttat aagaatttaa
     1441 tagcaatttc attttaaatt gaataagttt taaagccaaa gacaaaacta tgaaatatag
     1501 aataagacta aaggacagaa aggaattttg attatatgga cggcaatatt tcaatttatt
     1561 ataaaggtga tatatctaaa aaagaagata ttaaatttaa aataaaagaa tctgtaaata
     1621 atacaagact ttctagtaga atagagtata aggatgagga aaaaactttt gattttgaaa
     1681 gatttaatgt aattgatgct atttatggaa ataaaccatt tgaaaaagat ggaaaagtta
     1741 ttgtttttaa tggaaatata tataactatc atgaaataaa agaagaatta attaaaaaag
     1801 gacacaattt tactacaaat gatgatattg aagtcttatt ggcatcttat atagaatttg
     1861 gtaagaattg tgtacataag attaaaggaa tgttcaactt tataatctat gatagagaaa
     1921 atgaaagtat atttggagca agagatttat ttgggataaa accactttat tatataaata
     1981 aggaaaatgc aataattttt tcctcagagt ataagttttt acttgaatat atgaaaaatt
     2041 taaatataaa tgaaagaagc ttacaaagtt atttttcatt tcaatatgta cttcctgaag
     2101 atactatgat acaaggaata aggctgatac cagcaggtca ctattttagg gtagaaaatg
     2161 gtatactatc tttaaagaga tataataaac ttgagtttag aagtagtaca aaattttttt
     2221 atactaaaaa tcacttaggc aatagagatg tgaatgagga tgatataaga aatatagtag
     2281 tagattcaat agtaactcat atggaagagg agaaagaaat tggaacattt ttgtctggtg
     2341 gaatagattc atctattata actactgtgg cttcacaaat taatccaaat ataaaatctt
     2401 tttctgcagg ctttagtgtc aaaggatata gtgaattaga agtagcaaag aaaacagctg
     2461 ataagttagg tatagaaaat atccagatta atataacaca agatgaatat ataaaatcat
     2521 taccgaatgt aatttattct ttggatgacc ctatagcaga cccttcagaa gtaggtcttt
     2581 atttcttaat taaagaggca ggtaaacatg ttaaagtagc attgtctgga gaaggagcag
     2641 atgaattatt tggaggatat aatatatata aagagtatag tactatgaaa tcagttgtca
     2701 attcacctac ttatattaaa agtatattag gaagagtttc agaacttatg ccaaacataa
     2761 aaggaagaaa ttatctttat agagctacaa caccattaga aaaaagatat ataggaaatg
     2821 caaaagtttt tgaaaacagc gaagtaaaaa gattcttttt taagtataag gaaaagaata
     2881 tatatgaata cctgttgtct aatttgtata gagatgcaca aaaaaataat tacgattata
     2941 taagtaaaat gcaacatata gatgtgaata catggcttca aggcgatata cttcaaaaaa
     3001 ttagcaaact ttctacagct gagcaggttg aacttagagt accattttta tataaagatg
     3061 tatttgatgt agcaaaaaat ttaagaatgg agcaaaaaat aaataagaat aatacaaaag
     3121 ttttacttag agaggcgttt agagaaatag tcccagaata tgttgttcaa aggaaaaaat
     3181 taggatttcc aactccaata agagtttggt taaaagattc tctaggggat attgtaaagg
     3241 aaactattta taattcaaat gtagatgaat ttatagataa aaaatatgca ataaaacttt
     3301 tagatacgca tcttaaaggt catagagata attcgagaaa aatatggact atatttacat
     3361 tttgtctatg gcatcaatta tttattgagc ataaaaatgt cgagtattaa tacttttttc
     3421 aatattaatc agagggcaaa agcatgcaat gttttcaccc tcttgattat tatggaatag
     3481 gataagtaga attttgattg attagtttac tatctcaata aaattattgg caaatatttt
     3541 gacaagatta ctttcaacag ctaaatttaa gttttgtcca tcatcatatc cagcggaaat
     3601 caatcctagt aagtttccaa acatatctaa aagagctcct cctgaactac cactagagat
     3661 tggagctgta aattgtatca tttgaaccgt ttcaaaatca cgaaaagctg agataattcc
     3721 atctgaaact gtattaaaaa gacctaaagg gcttccaatt gcaactattt tttgacctct
     3781 cacaggtttt ttactgactt taacaggtat tggtttgcat tttctatcaa cctttatcac
     3841 agctaaatca taatctgaat ggtattttat aatttggtaa gatgtataaa ctttatcatc
     3901 attttcaaat ctaactaaaa atgaataccc atcattgact acatgaagat tcgtaagtat
     3961 ataaccttca ttatttatta ctactccaga accactctta aacggtttat tttcgttatt
     4021 gcacacaaca atcataacaa cagaagctga taactttgca aggtcttcat aagaaagttc
     4081 tacttcctca tcttgtgcaa tttttttaga tgttagattt gtgttattaa gtgtattttt
     4141 agagttaggt ttattcgtag taggagaagt cttattggta ttttcactgt aatttatatt
     4201 tgtaatatgc ctttcaaaag gtatttctaa tatatttgga gttttcctag aaagttcaaa
     4261 tagagtagaa aattttgaac attcaggata taaatcaaga atatctttat ttggatttat
     4321 atataaaata tcacacccaa gttgtgataa aaaatataag aaaaaaagtt cttgcctttt
     4381 aatatttcca aagtatacta tctttggtga aatagtagtc ttcaccttgt tataaaataa
     4441 tttaggtaga aaatagtcca tccaacacaa aagtttaact gcaaagtttt tgatgataga
     4501 ggatgtacaa tttggcatac ttgtggaaaa taaatctaaa gtatttttaa atgcaatctt
     4561 tttagtccaa tttaaagtgt cattttctat tttacatgga aagtcgatat tatataaatc
     4621 ccaactgtta aacttagaac tatcataaat attaaaaatt tt
//
LOCUS       DAEPLF010000238         4656 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEPLF010000238 DAEPLF010000000
VERSION     DAEPLF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.239"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(97..654)
                     /locus_tag="KR314_003220"
     CDS             complement(97..654)
                     /locus_tag="KR314_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBH0329578.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(660..1736)
                     /locus_tag="KR314_003221"
     CDS             complement(660..1736)
                     /locus_tag="KR314_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBH0329579.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(1737..2420)
                     /gene="modB"
                     /locus_tag="KR314_003222"
     CDS             complement(1737..2420)
                     /gene="modB"
                     /locus_tag="KR314_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBH0329580.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(2569..3381)
                     /gene="modA"
                     /locus_tag="KR314_003223"
     CDS             complement(2569..3381)
                     /gene="modA"
                     /locus_tag="KR314_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0329581.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKTDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEVESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            3574..4503
                     /locus_tag="KR314_003224"
     CDS             3574..4503
                     /locus_tag="KR314_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329582.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
BASE COUNT         1536 a          819 c          542 g         1759 t
ORIGIN      
        1 tttttatttt aatgtattat ctatttaaag attatatgta aatgctctta atatgattgt
       61 ataaatttta cttgtataaa ctttatacaa tcatatttat tcttctattt tctttattac
      121 tctacatgga tttccaacag ctacagtatt tggtggtata tcttttgtga ctacacttcc
      181 tgcacctatt actacattat cacctatagt aattcctggt gttatgatta ctcccccacc
      241 tatccataca ttatcaccta tttttacagg agaaccatac tctatgcctg aatttctaag
      301 ttgtgcatca attggatgat aggctgtata gatttgaacg tttggagcta acattacatt
      361 gtcacctatt tcaattttac atacatctaa aaaaatacaa tcataatttg caaagaaatt
      421 ttcgcctaca tgtatgttat atccataatc acacctaatg ttttgttcta catttatttg
      481 cttgccaact gaaccaaata gttgtcttaa aatatcttct cttttttcat attcatcctc
      541 taaagtatta ttaaacaatc tagttaattt tttacaatac tctctttctt ttactaacaa
      601 ttcatcattt gcatagtatc cttttccaga caacattttt tctttttcag tcatatagtt
      661 tactcctttt tgttataatt gtatctaaag caaaataaat attcctgtgg aaaatctaaa
      721 tatattctgt cacctttctt aaatctcatt ttactttttt ccaattctat ttgaataggt
      781 acacattcgt tagataaatc agtttttcta acatatatag tatatgtaaa aggattttct
      841 acaatgttaa tcaattcgaa acataacttt tcatttaaat cttcattaga atccaagacc
      901 ttaatatgat gctccctgat tcccacatat tctatattat cacctatttc tctatttaat
      961 gtcaactcgc atccccagtc tgtagcataa atagtatttt tatccagtct atttaatttg
     1021 gatatatttt tacaccctgt tatcttagct tctataaggt actttggaga ttcaaagagc
     1081 tcttttttag gtctctttgg aagaccttct cctttgttgt acactataat atcatcacaa
     1141 actcggtaag cttcttcaat atcatgagta acaaatagta tatctccctt gtaatctttt
     1201 aacatattca taagttcttt ttccatatta cttcttaaat gatggtccag tgctgagaaa
     1261 ggttcatcta aaagtaatat atctggagat gttgccaatg ctctagctag tgctaccctt
     1321 tgttgttgac caccagataa ttgccaagga tatctccctt caaacccatt taatttaagt
     1381 atatctaagt accttgctac tatttccttt ttttcactct tacttagttt taaaagccca
     1441 agttctatat tttgacttac agtcatatgt ggaaacaaag catagttttg aaataaaaat
     1501 cctacctttc tatcttgtgt tgataaattt atcttctttt cagaatcaaa tagaactcta
     1561 tcatttaata caattttacc tcttgttggt ttctcaagcc ctgctataca ttttaatgtc
     1621 atactttttc cagaaccaga ttcccccaaa aaccccagag tacctttttc ttgtttaatt
     1681 tctaccttta gtttaaaaga ggataaatct ttttcaatat ccacgtataa gctcaactat
     1741 ccacacctct ttcctataat tttttgctga aattccgacc aataatttag taataaaatc
     1801 attgctatag atattgctac aataatcata acccacatca tagctttatt catatctccg
     1861 ccttcaacag caaaaaatat agctattggc atagtttgag tttttccagg tatatttcca
     1921 gctatcataa gtgtagcacc aaattcgcca agagctctgg caaacgacag caccaatcca
     1981 ccaattattc caggccatgc taatggaact gcaatcttaa aaaatacttt ccattcatta
     2041 agacctaatg ttctagcagc tgacagcata ttattatcaa tctgttcaaa agctgacctt
     2101 gaagttctat acatcatagg aaaagacacc actactgctg aaattacagt tgctgtccaa
     2161 ctaaatatta aagtcctatc gaaatttaat aaaaactttc ctataaatcc attcttccca
     2221 catagtaata ataaaaagaa acctacaact gttggtggca aaattagtgg aagtgtaaat
     2281 agtccatcta taagaccctt ccacttacct ctatagttag ccataatata tgacacaaat
     2341 attcctatta caaaagttat tattgttgac aaaatagatg tcttaagtga tatccataag
     2401 ggtgaccaat ctgttcccat ctttccctcc ttaattttaa atggaaatag tgaaactaat
     2461 aactctatta atttcactat ttccatttta ttataataca caattatttt caattttcat
     2521 ttatttatat ataatttata cttaattaca cataatttat ttataagttt attctacttt
     2581 tttatatcca aattcttcaa aaatcttttg tcctgactca cttaataaaa attcttcaaa
     2641 tttcttagca gcatctacgt ttttactagc tttaattact gaaactggat atgtaatagg
     2701 tgaatgagtt ttttcgtctg ttttttcaac tacttttatc ttatcattat tgacagtatc
     2761 actgaagtaa acgaatccaa catcagcatt tcctgattgc acccatgcta aaacttcttt
     2821 tacatctttt gcaaatacta gcttatcttt taacttatct tttaaattta aattagttaa
     2881 cacctcatct gcatacttac cagctggaac actttcaact tcaccaacag caattttctt
     2941 cactttatct gtagtcaaat ctttcatacc tgatacacta ctgtcagcag aagaaataag
     3001 aactaaatca ttttttacta aatctttcat agtatcagaa actaataact tctcttcatc
     3061 caaagctttc atttgttttt gacctgctga aataaataaa tcacaaggtg ctccttgttc
     3121 tatttgttgt tgtaatgagc ctgatgctcc atagttaact gtaagtttta catttgaatc
     3181 aactttttta tattcttctt caatctttgc cattgcttct tttaaacttg cagcagccga
     3241 tatatttaac tctactgaat cactactttt agttgttttt tcttgttttc catcatctgt
     3301 tttgtcttca ttactattgc acccaactgt ccccaaagtt aaacatgcaa ctaatcctaa
     3361 tattcccaat atttttttca taacaatctc cctttttgta aatttgatac acattttaat
     3421 atgttttatt atgttttatc atgttttgtt atatatagtt taattatata tacatgataa
     3481 taaattttca agtaatttat ttacactact tttaaatatt ttgctacatt ttcctttttt
     3541 taatgttaaa ataaccttat atggaggtgg attatggact cattatcatc tttgacacct
     3601 gtcgaggtag ctgaattatt aaagataaca aaaaatactg tatatgaatt aatcaaaaga
     3661 ggtgaacttc cttcttataa agtagggaaa aaaataagag ttgatactaa agatgttgaa
     3721 gaatatatca acagccaaaa aactggtaaa gtaaaggata cacatttcaa caataaaaat
     3781 atagatataa ataataattt caaaattatt atttctggtc aagatgtaat attagacatt
     3841 ttagctaggt ctgttgaaaa aaaattggat ggaataagta catttcgctc ttatataggt
     3901 agttataatg ctttgtatga gttatataat ggcagagttt ctatagcatc atcccatcta
     3961 tgggatttcg aaacggatga atataatagc acttttgtta aaaagctatt gccaggaata
     4021 ccatgtgtac ttataaatct agcttataga atgcaaggtt tctatgttgc taaaggaaat
     4081 cctaaagaga taacaacctg ggaggattta acaaaaccaa gtataaccat tgtaaatagg
     4141 gaaaaaggtt ctggaactag ggttttacta gatggtaaac ttagacttct aaactttaat
     4201 ggtaaacata taaaaggtta tgataatgaa gaattatcac atcttggagt tgcaagtact
     4261 gtttctcgtg gaatagctga tgttggtttg ggaaatgaaa aagctgcttt acaagtaaat
     4321 aatatcgact ttattcctct tcacaaagag cgctatgatt tagttattaa aaaagaagac
     4381 ttacaaaatc cagtttacca aactataatt aacataataa actctcctga gtttaaagct
     4441 gaacttcaag gtcttggcgg atatgattta actgatactg gaaaaactat agcaaaagtt
     4501 taagattaaa atcgcaatct attttataag atttataatt tttcacaaaa tttataactt
     4561 aaacacgaaa taaattgcga ttttagtgtc tcaaaaaatt tatgtctttc attttatcat
     4621 ccaatacaaa atataatata attaattgtg caatca
//
LOCUS       DAEPLF010000239         4637 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEPLF010000239 DAEPLF010000000
VERSION     DAEPLF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.240"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..483
                     /locus_tag="KR314_003225"
     CDS             <1..483
                     /locus_tag="KR314_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329583.1"
                     /translation="SAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQFM
                     GVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLFFI
                     PYFIAIINSIISLLFLNQVLNNSFRLENLGILLLISSIYSLYFYLLKKIYTRKVWFFD
                     "
     gene            602..1993
                     /locus_tag="KR314_003226"
     CDS             602..1993
                     /locus_tag="KR314_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0329584.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            2100..3140
                     /locus_tag="KR314_003227"
     CDS             2100..3140
                     /locus_tag="KR314_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBH0329585.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            3629..4282
                     /locus_tag="KR314_003228"
     CDS             3629..4282
                     /locus_tag="KR314_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBH0329586.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIHGIIDGMKF"
     gene            4358..4567
                     /locus_tag="KR314_003229"
     CDS             4358..4567
                     /locus_tag="KR314_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329587.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
BASE COUNT         1884 a          444 c          794 g         1515 t
ORIGIN      
        1 agtgcaaaga taactgaaaa tttaaaagaa aaacatttta atttggatag agaatataac
       61 aggtcatatt ttttaacatt accagatatg tatttagtag agttacaaca aagtaaaatt
      121 cttcaattta tgggtgtttt tataggagtg atattattta tagcagtttt tagtttttta
      181 tacttcagat tatatacaga cgagatagaa gataatgtaa agtacaaaaa actatcaaaa
      241 ataggtctat cttttaaaaa tatgaataag attgttagta tagagatagg tacattgttt
      301 tttataccgt attttatagc aattataaat agcataattt ctttactgtt tttaaatcaa
      361 gtattaaata atagttttag gttagaaaat ttgggtatat tactactgat atcatctata
      421 tattcacttt atttttactt attaaaaaaa atttatacta gaaaagtttg gttttttgat
      481 taaagtggta tataatagat atataaatta atatttaaaa agtagttaat attaggggag
      541 ggcatttgca cctcccttgt aatatgtgga aaattgaata ttagaagaag gaggagactt
      601 gatggaaaat caacaattat taggaacaga acgaattagt aaattactac taaaatattc
      661 tataccagct ataataggta tgttggtaaa tagtttatat aatgtagtag atagaatttt
      721 tattggaaat ataccagggg ttggaccact tgcaataaca ggtcttggtg taacaatgcc
      781 aataatgaca ataatacttg catttgggat gttgataggt ataggaacta ctactaccat
      841 atcaataaaa cttgggcaag gtaaagtaga agaggcaaga aaacttatag gaaatgcaat
      901 gacgttatct gtaataacag gaattatcat aatgatactt ggaatattat ttgcaaataa
      961 aatacttaca ttgtttggag caagtgaaaa tactttaata tatgcaaaat catatataaa
     1021 tattatatta ttaggtacag tagtaaatct tctttccttt tctttaaacc actcaataag
     1081 agctgatggt agtcctaaaa tttcagcagg aataatgata gtaggatgtt taactaatat
     1141 agttttagac tggatattaa tatttggatt caatttggga atacaaggag ctgcaattgc
     1201 tacagtaact tctcaggctc ttacagctat tttaactata gggtattata taagtggaaa
     1261 atcaaactta cgattcagta aatctaatct aaaattagat aaaaagctaa taaaagcagt
     1321 gtttgcaata ggtatgtcgc catttgcgat gcaacttgca gcaagtttag ttcaagtaat
     1381 atcaaatatt gcacttaaaa ctcatggtgg agatttagct ataggggcta tggctactat
     1441 ttcttccatt gcaatggtat ttttgatgcc tatctttgga ataaatcaag gagcgcaacc
     1501 tattataggt ttcaattatg gagctgaaaa atatgatagg gttaaaaaag catatttagg
     1561 ttcattagta gtagctacta taattttatg tatgggtatg gtagtagtta tgcttttccc
     1621 agaagctatt ataggtatat ttaataaaga ccctgaactt atgaatatat ctgttaatgg
     1681 attaagaata tacttactta tgttgccaat tgtaggctta tcagttacag gaacaaactt
     1741 tatacaatct ataggaaaag caaaaatggc tatgttattg agccttttaa gacaagtaat
     1801 tttattgata ccagcagtat taattcttcc tacattctta ggtttacagg gtgtatggac
     1861 agctcaacca gtgtctgatt ttatagctac agtaattaca ggtatagttg tatttagaga
     1921 attaaaaaga tatactccta aaactgaaaa attaaatgaa aatgaaagac taaatgagat
     1981 aactactgag taaaaatata aattcagata attgagcaag aaattgcaaa aaatacgatt
     2041 atatgataaa ataaaaccgc tgtcaaggat aagagaaatt ctaattatgg agtgatttta
     2101 tggaatttac aaagaaaagc aaattaaaaa atatgtatgt aaacatgaat gtagctagaa
     2161 tgttgtctaa gataaatgag tataagggta ggcaactgct ttacaaaaaa caaccaaaag
     2221 aaattttaga aaacctagaa aaaaaatctt tagtagattg ttctgaatct acatacattg
     2281 gaaatcaaag agatagtagt aattttaatc tagaaaagtt gataagtaat gaagttacac
     2341 ctagaagtag agaggaattg agtattgttg aatacagaga tgtggtaaaa actattaaca
     2401 gtgcttatga gagtattccc atcagttcac aaacgattct tgagcttcat gggtacttat
     2461 ataaattttc atctactaga ggaggaagtt ataagtcaga taatgatttt attgaacaca
     2521 atttaaaacc tagcgaattt ataagtattg attcaagtgt caataaagtt gaaaaagctg
     2581 tagaagaaat atgtgaagct tataatacat tgatagagga agatgaaatt gatatattaa
     2641 tattaatttc tgcatttgtg ttagatttca tattgataca tccattcaaa gaaggtaata
     2701 taaaaatggc gagagtactt atcctattgt tactaaataa aaatgggtat gaagttggaa
     2761 gatatataag tttaggaaag atatttgatg atagttcata tgaatattat agtaatttaa
     2821 attatttgaa agcctcaata ggtagtgaaa aagcagatat gaatgcatgg attgagtatt
     2881 ttttagagac gatattaact gcatatgaga agctggatga tagtttaaat atatctgata
     2941 agaaaagaca aacaaaaact agtagaatag aaaaaataat aaattctacc cttggatatt
     3001 ttacaaaaga agatataaga gatttatgtc ctgacattcc agaacctact ataaatagag
     3061 tttttaataa tctaagaaaa caagataaga tagaagttgt tgcaagagga agaagtgcaa
     3121 aatggaagaa aaaatattaa aaaatgttta aataactaaa atggtatagt gttttacact
     3181 ataccatttt taatgtcatt agcatgagca ataatttaac aacttaagca atatcaattt
     3241 tatttattga ttatttttga actgaaaaat ggggttatat ggtatactac aataataatt
     3301 aattttggat gagaatatat aacgttacag aatatcttag gagtatatgt ataaaaaata
     3361 taataaaagt gtgaggttta gtaattaaca aattatgtgg cttcacactt ttttataaaa
     3421 taaaaatact tagcagaaga atattttagt tgatatgttt ataagatagt acttgtttgg
     3481 gtaaaagtat aaatagtaaa aaagtagaag taatgtgtat aaaaacataa gttacattct
     3541 gtaataatag ttattttata attcaacaaa agaatagaat gggtatattt gtgaaataat
     3601 aatataaaat aatcttagga gtgatcttat gaagaaaaaa ttattatata taaatgtaaa
     3661 ttcaaaacct gaagacttat cttcaagtaa aactgtagca agaaaattta taaataaatt
     3721 tatggaaaaa aacaaagatt ttgaggttga agagatagac ctttataaag aacatatacc
     3781 gagacttgag tatcagtact ttgaaaaaag aaatagcata gttagtgaag aaaatgctaa
     3841 aaatttagac gataaggata gaaaagagct tacaaagatt agaaatcttt gtgaccaatt
     3901 tgtgagtgca agtgtttatg taatagcagc acctatgtgg agtttatctt ttccagcacc
     3961 acttaaagag tatatagatt gtataattca agatggaaaa actatatctt ttgaaggcaa
     4021 tgataaacca caaggaattt taaatgatat tgatagaagt atggtatata tacaatcttc
     4081 tggtggacac ataccatggg tcttgaagcc agttatgaat aaagggttaa attatgtgga
     4141 aagtattatg aaatttatag gaattaaaaa atttgatgaa ttgttagtag atggaactgg
     4201 aactagtgaa gaagaaagac aagctgcaat agaaaaagca tctgaaaaaa tacatggtat
     4261 aatagatggt atgaaatttt aattataata ttttattaaa aatataaagg aaaaacctct
     4321 tttaatgtag aaacttatat tgaaggaggc gagaatcatg cgagaagaaa aaagtaatga
     4381 aaagtatgat tgttattggt gtaatcaaga aaataatttc tgtgtagaaa taaaagataa
     4441 tatagtaatg atagatgatg gcactggtac gttaaaacaa gccgttttca tagggtataa
     4501 acaaatccaa attaatttaa attgttcaca ttgtcaaaac ttaaatagaa taaaattaaa
     4561 tttgtagaat tttaaaatgg aaattattga aggaattaaa aatagaaagt gttaatattt
     4621 agactgaaaa ttatatt
//
LOCUS       DAEPLF010000240         4635 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEPLF010000240 DAEPLF010000000
VERSION     DAEPLF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.241"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(204..1388)
                     /locus_tag="KR314_003230"
     CDS             complement(204..1388)
                     /locus_tag="KR314_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0329588.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="KR314_003231"
     CDS             1761..4112
                     /locus_tag="KR314_003231"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBH0329589.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERFEVQGNKKMKNFPFLMGQGVWKGSDDLVPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..>4635
                     /gene="nrdG"
                     /locus_tag="KR314_003232"
     CDS             4134..>4635
                     /gene="nrdG"
                     /locus_tag="KR314_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBH0329590.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLV"
BASE COUNT         1784 a          544 c          753 g         1554 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttactt tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaatttg
      241 tctaatccag ataaaaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtattggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caagattgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagaacat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaatac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actatttcct aattttagtt
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgct ttgaggttca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta gtaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga ggcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatac ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttg
//
LOCUS       DAEPLF010000241         4563 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEPLF010000241 DAEPLF010000000
VERSION     DAEPLF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.242"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            614..1921
                     /locus_tag="KR314_003233"
     CDS             614..1921
                     /locus_tag="KR314_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329591.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLVLDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQSKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    2007..2269
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2395..3198
                     /locus_tag="KR314_003234"
     CDS             2395..3198
                     /locus_tag="KR314_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0329592.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINKTLKEMKKDGTFDKIYDKWFK"
     gene            3236..3847
                     /locus_tag="KR314_003235"
     CDS             3236..3847
                     /locus_tag="KR314_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH0329593.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3879..4550
                     /locus_tag="KR314_003236"
     CDS             3879..4550
                     /locus_tag="KR314_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBH0329594.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
BASE COUNT         1832 a          413 c          823 g         1495 t
ORIGIN      
        1 gaatattatc tagttacaat tatttataac taagataata aaacaacaat ggtgctaatt
       61 tatatgtata aattaattat gaaaaattta tataagtaaa atatattaac ataaaggtaa
      121 aattttgtat actaaaaggg aatatttaca gtttaatggc agttttaaaa taataaatta
      181 aagctgtcat ttttatttaa aaatttttag aaaaagtaca atagtgttaa tgtgtgaatt
      241 agataaggtg tatataataa tttagatgat atgatataaa atatgttgta aattaaatgt
      301 tttctttgac aatatataca caaggatgat aatatctata tgaataaaag atagtgtgta
      361 atttgataat atgtaattat aacattatga tataattaca ataaagtatg taataatctg
      421 tagaaatttc aagctagtta tctaagtttt cggataacta aatctaaacg gaatacataa
      481 tgtattaaat aaatgatgat actaaaatga tactattttt atagaaattt attgaggtat
      541 ataattgatg aaaataatat attaaattat ataatatatt gagttttaca taaaagcgaa
      601 ggtgggaata aaaatgaaag aaattgctga tgctataaca tcagagtatt taagctatgg
      661 tatcaatgaa gatattagta tctatgaagg tagattttgc atatatttag ataaaaaata
      721 tagatgtaat gggaaaattt attataaaat gacacctcca atatctataa gttttaaagc
      781 agacataggt tgtgttgaag agatagacaa tgaagatgac aatttagttt tggattatga
      841 taatgctatt cttgaagttc atggatacaa aattatttct ataacaataa atactttaag
      901 tgaatttagt gtagaaggtt atatcaatga tgactgcata aaatctaaga actcctatgt
      961 tgaatatgta gattttaata taataaactt agataaaata cctggaaaac taattaaata
     1021 caatgataag gtatacgcag gtagaattga atttgatata aatgattatg ttgtaactat
     1081 agacaagcga tatgattata gaaaagaatt aaaatcagaa ttaaagtcta aatctggagc
     1141 aataataact catataggta ggattagaag aaaagatggt agaattttta gaacaaataa
     1201 tacaataaat cttttagata gaatatcaac agctttgagt tttatgtgtg gaagatatgt
     1261 agggttttgt ttggccaaag ggtatagaag tggtaatgaa gtttatagaa tatggaatga
     1321 aaatcaaata tctcctttta gatatgtacc aacatggtca gatacacttt caaattatca
     1381 taatatggaa aaatatataa gccttatgtg taaaaagcta gaagattttt attatggttc
     1441 tgctataaag agtgttgttg attggtacat agaatctctt ggaagtgcta ccatggaaaa
     1501 caatataata tctgtacaaa ttgcacttga aactttgtca tatgtaattc tagtggaaca
     1561 aagtaagata cttacagatg aagcatttga ctgtaatttg gcatctaaaa atataagact
     1621 cttacttgat acatgtaaga taccatatgg taaacatgaa ttaaatatat ttgataatat
     1681 aataaaaaat aaatttgatg atggagtgga tttagttatt tatttaagaa atagcatagt
     1741 tcatccaagt agaaagactc atagagctgt gttagaggtt gaagatattt ggaatataat
     1801 ttcaatagga acgagatata ttgaacttgt tttactcttt attttaggtt atagaggaga
     1861 atattcaaac agattagtag aaaggtgtta tggtgaagtg gaagttgtac cttggaacta
     1921 gatttttata tttattttaa aatatatatt gacatatatt gccttatgct ataaaatcaa
     1981 tatcataata ataattcaga ataacattaa atgctaagaa aagaaatagt aaaggtagtt
     2041 aatcttaaac agagagtttc cgattggtga gaggagcaat agattttact ttgaacacat
     2101 ctttgagcag gactctgaaa gagtagaaat actttattag gagatttccg gaatccttgc
     2161 acccgttata gtgctagagt ataataattt tattgtactt aaagaggtta gtgtcgtgag
     2221 gcattaatga acagaggtgg aaccacgaga gttaactccc gtcctctagc agaagctaga
     2281 ggcgggagtt ttttaatata attttttaag cgcgcagaga attatattgc aatagataca
     2341 ctttttaaaa aataatcaaa atattttaaa aattaaaagg cgagggagaa tattatgaaa
     2401 aatatattaa aaaaagtagg aatatttaca attatgttag gtttattagg tggagttgta
     2461 ggttgttcaa aaccagataa tgaaaaagat aaggatgctt caaaggaatc taaaaaagaa
     2521 gtagttgtag ggtttgataa tacgtttgta ccaatgggat ttttagatga aaagggagat
     2581 acagtaggat ttgacgtaga cttagctaaa gaaacattta aaagacttgg aatggaagtt
     2641 aagtttcagc ctatagattg gtcaatgaag gaaactgaat taaatgattc aaaaactgta
     2701 gatgtattat ggaatggata ttctataaca gacgaaagaa agaaaatagt atcatataca
     2761 gaaccatatt tacaaaataa acagataata gttactctaa gtgattcaaa aataaattca
     2821 aaagctgatt taaaagataa agaagttgga acacaacaag gttcaacagc acttgatgca
     2881 gtggaaaagg ataaagattt tatgaatagc ttaaaaggtg gagcaccagt tctttatgat
     2941 acttatgata aagcacttag agatttagag ataggtagaa ctagtgcagt tgttggtgat
     3001 gaagttttaa ttagatatta tatgggacaa aagggtgaag acaaatacaa ggtattaaaa
     3061 gacgattttg gattagaaga ttatgttgta gcaacttcta aagaaaatcc agaactttgt
     3121 gagaaaataa ataaaacatt aaaagaaatg aaaaaagatg gaacttttga caaaatttat
     3181 gataagtggt ttaaatagaa gataagttag ctgttaagaa gggggcacta aaggcatgtt
     3241 acaaggttta gagatagtta tagcaatgtt ttgtataaca ttgatagtat caattccatt
     3301 ggggattgga gttgcatttt taagattatc aaagaataaa ttagtaagtg gtattacaca
     3361 atgttatata ttgataatga gaggtacacc tttacttttg caaatgatag tcatatttta
     3421 cggattacct cttttaggaa ttgtatttga cagatttaca gctggagtag tagctttctt
     3481 cttaaattat gcagcatatt ttgcagaaat atttagaggt ggaatacagt caatcgatag
     3541 aggacaatat gaagcttcaa aagtactagg atttgataaa tttactatgt ataagagagt
     3601 aatctttcca caagttttta aaagaatact agcacctatt tcaaatgaag taatcacatt
     3661 agtaaaagat acatctttag tatatatatt aggattaaat gatattttaa gaatatctca
     3721 aatagcaatg aacagggaag caagtctgtt accattattt gaggcaggag cgatatatct
     3781 tatatttgta gctatactta ctaagggatt tgaattactt gaaaaaaaat attcttatta
     3841 tagataaagt tacaatatta taggaggagg ttttttatat gttgaaaatc aaaaacttaa
     3901 ataaatcatt taaaaaaaat agagtactta aagatatttc ttttgagtta gaagaaggtc
     3961 agattggagt tttacttggt aagtctggtg caggaaagac cacaatatta agatgtataa
     4021 atggtcttga agaatttgat agtggagaga ttataataga taatgaggta ataaaaaata
     4081 agagagatat ggcaaaaata cgaggaaaaa taggtatggt ttttcaaaat ttcaacttat
     4141 ttcctcatat gacagtcctt gaaaatataa ttgaatcccc agttaatgtt tttaaagttc
     4201 ctagaaaaga ggctgaagag agggctagag aacttttaag attagttgat ttagaagata
     4261 aattaaattc ttatccattt gaattgtcag gaggacagca acagagagtt gctatagcta
     4321 ggtcatgtgc attgatgcca aaagtccttt gttttgatga acctacttct gcacttgata
     4381 ttgatactat tcaaagagta gtcaatataa tgaatagact taaagacaaa ggtatgacaa
     4441 tattaataat tacacatgat gttgtcttct caaataatgt tgcagataaa attataagta
     4501 taaaagatgg tatagtagaa aatgttcaaa taaaagaaaa aattgtttag agttttataa
     4561 ata
//
LOCUS       DAEPLF010000242         4558 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEPLF010000242 DAEPLF010000000
VERSION     DAEPLF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.243"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..1085
                     /locus_tag="KR314_003237"
     CDS             <1..1085
                     /locus_tag="KR314_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0329595.1"
                     /translation="NLILKDIADTLNELTNINETFARVSADNFNILLTYNKKEDIINI
                     IKKIIANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSIKNNSDVNFAFFNDKLRE
                     KLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEALVRWQYTEKEVIYPGDF
                     IPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVNFSRVDFLKKDFIENIVN
                     ICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFIVAMDDFGSGYSSVNMLK
                     NIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRVVAEGIETRSQIEMLKKA
                     NCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1118..4555
                     /locus_tag="KR314_003238"
     CDS             1118..4555
                     /locus_tag="KR314_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBH0329596.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPIFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEISKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENILGGIIITEFNDDFTIKYTNSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIIDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSEMVKTISNSIIHKDNKEVFLNTFLCKNLLSAYNKNITEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIKLKLKAERDTLTGLYN
                     KGTTENLINDFLNSDEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKHLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENTKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNNCIYQNELENNISDNNIKLANK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSER
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
BASE COUNT         1931 a          453 c          699 g         1475 t
ORIGIN      
        1 gcaatttaat attaaaagat attgcagata ctttaaatga attgactaat atcaatgaaa
       61 cttttgctcg tgtaagtgct gataatttta atatattatt aacatataac aaaaaagagg
      121 atataataaa tattattaaa aaaattatag caaataatga acttgtaaac ttatcttttg
      181 gaatttatga aatcaaagat aaggatttat ctgtaagtgt atatagtgat agagcttcac
      241 ttgcaaaatc atcaataaaa aataatagtg atgtaaattt tgcatttttt aatgataaat
      301 taagagagaa attattactt gaagataaga tagaaaaaga aatggaatat gctcttgaaa
      361 gtgggcaatt cgtaatgtat ttacaaccaa aatataatat taaattagat aaattttgtg
      421 gaagtgaagc cctagtcaga tggcaatata cagaaaaaga agtcatatat ccaggtgatt
      481 ttattcctat atttgaaaaa aatggattta ttagaaaaat tgatatgtac atattagaac
      541 aagcttgtaa agaaatccgg tctttatttg ataaaggaat ttcaccttta cctatatctg
      601 taaacttctc aagagttgat tttttaaaaa aagattttat tgaaaacata gtaaatatat
      661 gtgatagata taaaatacca tatagtttaa tagaaattga aattactgaa tctagtatgt
      721 ttggagatac tgatactcta tttaatgtta gtagaaacct acaggatatt ggttttatag
      781 ttgcaatgga tgattttggc tcgggatatt cttctgtaaa tatgcttaaa aatataccat
      841 taaatgtaat aaaattagat agaggatttt ttgttgatga taaagatgtt gataagagtc
      901 aaattgttat aaaaagcata gtttctctca ttaagcaatt aggaataagg gttgttgctg
      961 aaggaattga aacaagaagt caaatagaaa tgttaaaaaa agcaaattgt gatattgttc
     1021 aaggatatta tttttcaaaa ccattaccaa ttaaagaatt tgaaaaatta gtctataaaa
     1081 tatagtcagc gagtcttaaa tgggaggtgg tgattaagtg ataagagcaa aatcgttaaa
     1141 aaatgtactg agtattgttt taatattttt atttacaata atttcttttt ctgattttgc
     1201 atttaaaact aatgtccagg aagaagaaaa tagtaaaatt aatttaaggg aaatatccct
     1261 acaaagttcc aatgctataa aaataaaatt agatagttct ctatccttta ttaaaagtgt
     1321 ggcaaatatg gtttcttatt ttaatactat agataactca aaaatagttc cattattagc
     1381 taacatatca aaagaatctg actttcaaag aatctctatt gtatctcctg atggaataag
     1441 tcataccagc gatggcaaaa ttatcaatgt gtcaaaatat cagtatttta agaaagctat
     1501 taaaggagaa agcaatatat ctgaagttgt gaaatctcca attgatggaa aagatacttt
     1561 tgttatagct actcctatat ttaataagaa taacaaaata attggtgttt tatacggttc
     1621 ttactacaat gataaattag ctgaatttat agatataaca agttttaata gtgaagggta
     1681 catagatata tttgaatcta gtggtcaatt tgttttaaaa tcttcacata caaacaaact
     1741 gattaagtca agtagtaaca tatatgatat atttaaaaaa gcttcttttg atgatggtta
     1801 tagctataaa aaaatatatg aagatacaaa aaataacaag agtggcttta cctcatatga
     1861 atacaaagga gaaaatcgtt atgctactta tacacccatt ggtgtaaatg actggtatgt
     1921 aatttctgtg ataccacaat atactgtaag aagaaacatt gataaaataa atcaattagc
     1981 attttctctt attttcaaag tattcttatt gtttattatg cttataatat atattattta
     2041 taaaaacaat aaatctcaga aagatatact aaaagctaac aaagaaatct catctattac
     2101 aaatagcatt cctggaggag tacaaaagtg taccctgggt gaaacatgca atattaattt
     2161 tttaagtgat ggatttatag agttgtttgg ttatacaaga gaagaaataa aaacattgtt
     2221 taataatgat ttttatagtt taatacataa agatgatgtt gaaagaataa aaaaagaaat
     2281 ttctaaacaa ctttacaaaa ataaaactat tgaattagaa tataggatta taacaaaaaa
     2341 tggtaataca gtatgggttt tagataaagg agaacttata acagaacctg atggaaaaga
     2401 atatttttgt tgtgtaactg tagatattac tagaacaaaa caattagaac aagagatgga
     2461 acaaaaaaat ctagaacttg aaatgatatc agaaaatatt ttaggtggta taattataac
     2521 agaatttaat gatgatttta ctataaaata cactaactct ggttatcttt caatgattgg
     2581 atatacacgt gaacaattag aaaatgaact taaatacaaa gcttgtaatc ttgtttttta
     2641 tgaagataga aagaaggctc ttgaaagtat acaacatcaa ttaaacacaa aaggaatagc
     2701 tgtatatcaa catagaataa gaagaagaga tggtaatatt atctgggttt ctataaaagg
     2761 aaggcttatt attgatgatg atggtattga aaaaggtatt tggattctaa atgacataac
     2821 agatttaaaa aatgcagaac aaaaacttaa aataaatgaa gaaagattta gaattgcatt
     2881 aaagcaaaca tcaaatacta tatttgagta tgatataaaa acaaagtcac ttttaaatac
     2941 tagtaaagag cttgataaat ataatattta taaaattact aaaaacgttc catattctct
     3001 agtagaatca ggagttattc ataaagattt tagtgatgat tttataaaca tgtatgaaaa
     3061 tgtaataaat ggagcaaaaa aagcaagttg tgtagttaag atgaaacttc cagatgaaaa
     3121 ttatatatgg aataaagtta ccttgactac tatttttgat gaagatggta atcctgtaag
     3181 agcaattggt atacaggaag atataagcaa acaaaaggaa gcagagataa gatataatca
     3241 agaaggtaag tatcattcag ctatattgtc ggaagcaatg ataacctatg aaataaatgt
     3301 atcaaaagat acttttatca aaggaaatga aagttggcaa aaaccatttg gaattaagcc
     3361 tgacaataat tattctgaaa tggtaaaaac tatatcaaac agtattattc acaaagacaa
     3421 taaagaggtg tttttaaata catttttgtg taaaaattta ttatctgcat ataacaaaaa
     3481 tataactgaa ataaaattag aatatcgaag aatgaatcaa gaaaataaaa tgatttgggt
     3541 catttgcaca atgcacttaa taaaagattc tattacgaat gatataaaag cacttgctta
     3601 tgttaaagat attgacaagc aaaaaaatga agaaattaaa ttaaaattaa aagctgaaag
     3661 agatactctt acaggattat ataataaagg tactacagaa aatttgatta atgatttctt
     3721 gaactctgac gaagcaagct taggaatgca tgcattttta attgttgata tagataattt
     3781 taaatctgta aatgataatt taggacatgt atttggagat aaagtattat ttgaagtttc
     3841 taaacaccta aaacctatat ttagaaaaga tgatatagta ggtcgaattg gtggagatga
     3901 atttatagta ttcttaaaaa atattcagtc ttttgagaat accaaaaaga aagcagaaaa
     3961 aatatgcgat atatttagaa attcttatac ggaaaataaa aagacatata aaatatctgg
     4021 aacagttgga atttcatttg taccagaaca tggaactgta tttgaagaac tttataaaaa
     4081 agcagacata gcactttatt atgcaaagag caaaggaaaa gataagtatg cactttataa
     4141 taattgtatc taccaaaatg aacttgaaaa taatataagt gataataata taaaattagc
     4201 aaataaatgt gagaaatcca aaccagtact taaagaaatt cttgattcta ttgataatta
     4261 tatttatata gtgaatccaa atacttatga acttcttttt gtcaataata aggtgtctcg
     4321 tttggattta gagataaaat taggagaaac ctgttataaa tcactattaa aatctgagag
     4381 accatgtaat aattgccctc ttaacggatt agaagaaaat aagcattcaa aattcaaaat
     4441 agagacaaat acatttggta aaaatatcag tatagcaagt tggattaatt gcatagatgg
     4501 agaatacaac tgtcttatag agtgtataga ttcttcttta tacaaaaata aataaaaa
//
LOCUS       DAEPLF010000243         4557 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEPLF010000243 DAEPLF010000000
VERSION     DAEPLF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.244"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            103..1254
                     /locus_tag="KR314_003239"
     CDS             103..1254
                     /locus_tag="KR314_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBH0329597.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1300..2475
                     /gene="thiI"
                     /locus_tag="KR314_003240"
     CDS             1300..2475
                     /gene="thiI"
                     /locus_tag="KR314_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBH0329598.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2631..3434
                     /locus_tag="KR314_003241"
     CDS             2631..3434
                     /locus_tag="KR314_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0329599.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGIEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3677..4318
                     /locus_tag="KR314_003242"
     CDS             3677..4318
                     /locus_tag="KR314_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBH0329600.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1818 a          438 c          894 g         1407 t
ORIGIN      
        1 atttaagatt gtctttttaa taaagattaa caaaaaaaat actataatta gtggtataat
       61 agttagagtt gaagatttta aataaaggta ggtgtaatta agttggaaat atacttagac
      121 aatagcgcaa ctacaaaacc ttatcaagaa gttatagata aaatggttta tgcacttaat
      181 acggaatatg gaaatccttc ttctgtccat agaaaaggtg tagaagttga aaaagctata
      241 aaagaagtaa gacaagatat agctaaatct ctaggagcga aagaaaagga gatttatttt
      301 acatcaggtg gtacagagtg taataatact ataataagag gtattactag tttaaataaa
      361 aaaagaaaaa atcacataat atctactaac atagaacatc cttctgtttt aaatacatta
      421 aaagatttag aagaagatgg ttttgaagta acttatttag aggtcggaaa agatgggaaa
      481 ataaatatag aggatttaaa aaatgcgata aagtcaacta catgcttagt aagcatgatg
      541 catgttaaca atgaaatagg aactatacag ccaataggtg aagttggaaa gtatttgaag
      601 ggtttaaaag aaaaaattta tttccatgtt gatgctattc agtcatatgg aaaaattaat
      661 tttagaccat ctaaatacaa tatagatttt atgagtgtaa gtgcacataa atttcatggt
      721 ccaaagggaa ttggatttat gtatataaaa gaaaataatc gccttaagcc tatgttaaca
      781 ggtggtggtc aggaaattgg aattaggtcg ggaacagaaa atgtacctgg aatatatggt
      841 cttggagaag ctgttagaat actaaacaaa gatttagatg ttgttatttc gaaagtaaat
      901 aacttaaaaa atatattaaa aaatgaaata attgataata tagaagatat aaaaatcaat
      961 tctccagaag atggagtatg tcatatatta aatgtatcat ttagagggac aaaaggagaa
     1021 gttttacttc attacctaga gcaaaaaggt atttatgtat caactggctc tgcatgttct
     1081 tctaaaaaga aaggtagtta cgtattaaat gctataggtc ttacaaatga agaaataaat
     1141 ggaacaataa gatttagttt atctgatatg aatacagaag aagaaatgtt agaagctgtg
     1201 aaagttttga aagaatcaat ttgtgactta aggtcaataa tgaaaagaaa ataaagaata
     1261 ctgtatagat agtgataata aattagttta agaggtgaaa tggtgtataa tatattaata
     1321 gtaaagtatg gagaaattgg agtaaaagga aagaatagat atatatttga gaataggctt
     1381 ataagaaata taagaaatat gttaaaacca attggaagat ttaatgtata taaagagtat
     1441 ggaagaatat atgttgattt agaagactat gattatgaag aagtaattga agaagttaga
     1501 aaagtatttg ggatagtggg tgtgtgccct ggagtaagag ctaaaaaaga ttatgataca
     1561 ttaaaagaaa tagctttaaa aatgttagaa gaaaaaatag aggctggata taaaactttt
     1621 aaggtagagt caagaagagg agataagtct ttcgggttga cttctcaaga aatgagtatg
     1681 gatataggtg gatacttatt atcaaaggta ggagatagga ttaatgttga tgtaagaaat
     1741 ccagaagtta aaataaagtg cgaatacaga gaatttcata ctatggtcta tagtgatact
     1801 atacctggat atggtggatt gccacttgga acgaatggta gagctatgtc tcttttatca
     1861 ggaggtatag atagtccagt agctacatgg atggttgcaa aaagaggtat ggaggtagag
     1921 gcagttcact tccatagtta tccatttaca agtgaaaggt cacaggaaaa agttaaggat
     1981 ttagctaaaa tactggcaaa atactgtggt agagtaagac ttcataaagt taacatatta
     2041 gaaatacaaa aagcaattgg tgagaattgt aatgaagaag aagctactat tctttcaaga
     2101 agatttatga tgaggatagc acagagactt tctgaaaaaa gacattgtga tgctttaatt
     2161 acaggtgaaa gtattgggca ggttgcatct caaactatac aagggcttac ttgtactaat
     2221 gccgttgtag acttaccagt gtttagacca cttatagcaa tggataaatc agatatagtt
     2281 gatatagcca aaaaaatagg gacttttgaa acatctatag tgcctgaaga agattgttgt
     2341 agtgtatttt ctcctagaaa gcctgtcact aaacctagat tagagaaaat agaaaaatct
     2401 gaaacagcac ttgatattga aaaattagta caggatgcta ttgacaaaat agaggtagag
     2461 gatattgagt tttagcatac aagttattta attactaaat tgaatattga gtatattagt
     2521 agttctgtaa gtatattagt agttctgttg aaaggattta tgtagacaat ttattaatat
     2581 atagtattta aaaataagta ctataatttt atttaatggg aatgattatt atgaataaaa
     2641 agggttttac attaattgaa ttgttggtag ttatatctat aataggaatt ttagttatag
     2701 tagctgttcc agcgttattt agaaatatag aaaaaagtaa ggcagttaca tgtctttcta
     2761 atagagaaaa tataaagact caaattgtta ttgcaatggc tgaggaatca agtaaagaca
     2821 agaatgaagt cataaaagag gtattagaaa acaaagatgg taagtacttt gaaacagaac
     2881 caaagtgtaa gtcaggtgga atatattcag caacgtttga tgatggttat gatggaataa
     2941 ctggaataga aagcattgca aaagtgtatg ttacttgtac aaaacatcca gatggtattg
     3001 aaatggctag ggatatacat caaagtatga aagatttgat tgcatcattt gcacaagacc
     3061 cttctataat accaggagct tcaaagggca atgatgattt tagaaaatat ttattagaca
     3121 ataaatataa aaatgggtgg cctacaattc cagatgaatt taaggcaaaa tatggattaa
     3181 gtaaggatac actatatata caaccatatg catataatcc tactaaatct gatgctactg
     3241 tagttgtatt tgcaaataat aagactggag gtaattggta tacttcccta gtttacgatt
     3301 atgatgaagg tagatggtat aaaggtaaaa atggtatttc tgttgcaggt aggtcatggg
     3361 atgttgacac agatagtgtt aagtctgtaa aaacagagat tcattctaaa gagggatggg
     3421 gtcctttaaa ttaatatatc atgttttatt aatctggtat tgattaaact atagaaaaga
     3481 gcagtttcta tagatgacga ataccagatt ttcatttata tgtataatta agatatatta
     3541 aaagtattta tatttttaag tcttcacata caagctaaag ataattataa taatattgta
     3601 aagaaattta attataagat taattttctg aagatttagt atttgtacta agaaatacat
     3661 aaaaagggga atagatatga ataataattt tcaagacaac caaacaataa atttaataca
     3721 aagtcgtaga agtatacgaa aatttacaac agagcaaatt agtgatgagc aagtaaacac
     3781 attattacac tgtgcatttg cagcaccttc aggatgtaat aaacagcctt ggcacataac
     3841 tgttgtgcaa gaccagaaac ttttaaaaga gattagtgat gatactttat ctaggattca
     3901 tgaagtgagt aatgttgaga taaataaaaa ctttaagtta ttttatggtg ctccaaccgt
     3961 tttatttatt tcttatgatg aaagtagttc atgggctcca tatgatatag gaatacttac
     4021 agggaatata acaacagctg cacaagcact agggcttgga agttgtataa taggaatggt
     4081 tagaggttta tttacacctg ttgaacaagg ggatattgaa ggtcttgtga gtgtattgga
     4141 taaagaagat gtaaaggaat cagagtcaat aaagatgaag tttgatacaa ataaaaaata
     4201 cagagaacta cttgatatac cagaagggta ttctgtacca tttggtattg ctgtaggtat
     4261 tccagatgga aatttaccaa atgctagaga ggtagtttac aaagtttcca gagtttagga
     4321 taaagtattg caagaaccaa tcagttactt gaagaattaa accagtaata tacaaataaa
     4381 ttgatggaag aggaagctat ttaaaaagta gtttagattt ttcattgtaa aataattaaa
     4441 taaagaataa ggtgataagt attttattta ttatcttatt cttttttatt tttgttttgt
     4501 agaatgataa aaataaggat taagtgtaat aaaatagaaa acaagtatgt taatttt
//
LOCUS       DAEPLF010000244         4551 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEPLF010000244 DAEPLF010000000
VERSION     DAEPLF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.245"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..1368)
                     /locus_tag="KR314_003243"
     CDS             complement(<1..1368)
                     /locus_tag="KR314_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0329601.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVC"
     gene            1510..2406
                     /locus_tag="KR314_003244"
     CDS             1510..2406
                     /locus_tag="KR314_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0329602.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDFLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            3071..3211
                     /locus_tag="KR314_003245"
     CDS             3071..3211
                     /locus_tag="KR314_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329603.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(3208..3579)
                     /locus_tag="KR314_003246"
     CDS             complement(3208..3579)
                     /locus_tag="KR314_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329604.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            4128..4385
                     /locus_tag="KR314_003247"
     CDS             4128..4385
                     /locus_tag="KR314_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329605.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
BASE COUNT         1856 a          493 c          605 g         1597 t
ORIGIN      
        1 gcaaacaagt gtaacaataa tataaacatt tctcattcca aatacaaata ttttatcatt
       61 attaatcaca taatcattga ccctatatcc agctttaata ctcataaaat tatataaaag
      121 tgttgtagaa atagtaatac ctataacttg tcctaaattt cttacaaggg aatttatgct
      181 tcctacaact cctagcttac ttctagaaca gcttgacatt atcaaagcat tgttagcagg
      241 ttgaaatata gcttgtccta ttgatattat cgatatataa attacaaata aaataagggc
      301 agaactttct ttcaatcttg acattagaaa gaatccaaaa ctcataacga gaagaccaat
      361 taaaataatt ttttctgact tgattttatc tgatatattc ccaaatatag gtgaaaaaat
      421 ggctaaaatc aaaggtgata gtatcataaa taaacctgct tgaatagggg gaagtttcat
      481 agtactttgt aagtaaaatg gaattaaaat acttgatgat gcaatacaaa tgaatgatgt
      541 aagagcacaa attaaattca atgaaaattt aaaatttcta aaaatttcaa gatcaagaag
      601 cggttgagga tgtttttttt ctaatatcaa aaataatata ataaaaataa ttgataataa
      661 tagtgcaatt aatatatatg gatttagtaa tcctgtttgt tgagcactaa ttagagcact
      721 aaaaaataat gttgttccta aaaattgaag tatagcacca gttttatcca tattttctga
      781 agatttttta ctattaggaa gaaattttgt atttaaaaag attacaataa gaccgattgg
      841 aatatttata aaaaatataa cattccaatc aaatattgac aaaataaatc ctcccacaga
      901 tggtccaacc atatttccaa tagcaactgc agtaactaga atccctaggg cttttcctct
      961 gctttctttt ggaaatagtt ctgtgataat tccatgattg tttgccatgt aggcagaagc
     1021 gcctattcct tgaataaagc ggcaaactat aagtaaagtt aaagaattac ttaatccaca
     1081 tagtaatgaa gctgatgtaa agagaatagt acctacttgg aatactcttg atttaccaat
     1141 aatatctcca agtcttccaa aaaataatag tgttgaacaa ataataattg agtaacttgc
     1201 tattacccat tcaatagatg ctagagatac atttagtttg cttgcaagaa ctgggagagc
     1261 aacatttata atactcgaat ctaatgttgc cataaaagtc atggcaatga ctacaaataa
     1321 aactgtgatt ttttttctat tatcatcttt tttaaaagag tttgtcattt ttattacctc
     1381 cttattataa tgactggcat tatgttaatg tttacaatga tagttacatt ttatataata
     1441 gcgatatgaa ataatgaata caataactat attgcttata attagcacta ttttgatgag
     1501 gggggaataa tgaaggataa aaatacatat tttaatagga aaggtcaaga aaactttata
     1561 gatatttttt taaaggatga cattaaaaaa ggagcaaact ttaaaagaca ttggcatgag
     1621 catctacaaa tatattattt tattgatggt atagcatatc ttgaatgtgg attggataat
     1681 tttaaggtag tgcctggaaa tattgtaata gcaaacagta atgaattaca ttatttagaa
     1741 agcttatcag ataatttaaa attctatagt attcgtattg agcccacatt cctttttagt
     1801 aatcaagttg atttgttaca aactaagtac ttagcaccat tggctttaaa tcatattgta
     1861 tttaataatt taattcaaaa tgattttctg attttaaaat gtattacaac tatacttgat
     1921 gaatatcaca ataaagaagt tggatatgag ttatctataa aagctaatat ctatcaatta
     1981 atagtactct tattaagaag tcatattgat aaaatatatt ctgaagatga atttataaaa
     2041 agaaagtatt ctttagaaaa atttgaatgt atctttgact tgatagaaga aaaatatatg
     2101 gataaaatta cattaaaaga acttgctagt cgtgtgaact tgagtgcaca tcacttttgt
     2161 agaacattta agcaaataac aggtaagact acaatcgatt ttataaatgg aatcaggctt
     2221 gataaagcta tatattattt aaaggaaaca gatttaaata tgacagaaat tgctatgaga
     2281 tgtggctttg atagcataaa ttattttagt cgcttattta aaaggaatta taatatatct
     2341 ccaactaagt atagaaatga acatagagaa gttagtaatg ttattttaaa agaagatatg
     2401 aaataattca taaatatact ttatagaaaa tatatatttt taaatctttg agtttttctt
     2461 aataaatatt aataaagtat agtacataaa tgagtaagac tacttgtata gttaaatgtg
     2521 aaaatgagta tgaaagaatg tagtgtctat gaaaagtata ttttaatatg aaactaaaat
     2581 tggaaaaata agaatagcag aaaaaatgat tatattataa atcagttttt ttgagacatc
     2641 tggcttagat tctgaaagat atttaatcaa agaaataaaa ctatcaaata agcaagtaaa
     2701 cagctaaaat agtatgattt ttatagaaaa tttaaatcca tcctaaagaa ttaaaagaaa
     2761 gtcaaataaa tataaaaaaa ttatatattt ataaaaaatt ttataataaa aatcatcctg
     2821 aaaaatatat aaaaatagaa aaaacataca agattctaca ataatagatt tttttgttat
     2881 tattacgagt tttttattgc aactgtagaa taaaagtatt attatataat taagataatt
     2941 atctagggaa attttggata atcaaaatat attttataaa atcaaaatac aattacataa
     3001 actttattaa aaatttgttg ggaatttctg actagcatta agaacgtaag tttgttgggg
     3061 gatggtatat gtggaaaagt caaacactac tgttacaaaa aaagaaaaaa ttgaagatat
     3121 aggaatgaaa ttagaacatt tattgagtga agatattact ttttatgaat tattacttga
     3181 gattttgtat aaagaaaaag aagagaatta attctcttct ttaaggaatc tttttaaata
     3241 ggcattatca atcatttttt ctatagcttc tctatcttct ttttcaagag aatatatttt
     3301 ttctaagagc ttttttgttt tttcacttac tgataagttc tctatgatta atttatcttc
     3361 tttgcttatt tttgtattat catttacgcc taataaataa tctgttgatg tttctaatgc
     3421 ttttgctaat ctaactatta tttcactttt aggttctctt aaattatttt cataacgtga
     3481 caagctagcc tcagttatac cgactttagt agcaagttct ttttgattta tatctaattc
     3541 ttttcgtaga tttgctattc tatctcctaa actttccata tgtacatcac tccttaccga
     3601 aattataagt tattttgata aaaagtaaaa taaaattacc aaaaatataa aaaaaaaggg
     3661 ttaaaataat aaaatatatt gaaaattacc atttgtatat gtataatatg aattatacaa
     3721 atggtaattt gatgagaggt ggtatgttga aataagaaac tgaattaatt aataaatata
     3781 tagaaagttt tgaaaaccct taattgagat ataaacaata tgtacaaaat tttttgcaag
     3841 taaaattacc aaatgtataa aataaatcag tagcatagat aattttatgc tttaatacat
     3901 tagattattt aatttagtta taatggtcaa aaatgcaatc tacaaataaa acaaagtatt
     3961 atcaaaaaag tattctttaa gagaattttt tattagagat gtaatttata taaataatcg
     4021 tattaaaaaa taaaatacta agtttaaaaa tttaaataat ctataaatca attataaaat
     4081 attatttctt agaaattaaa atgaattaag taaatggagg aattgatatg ataataatta
     4141 gaagtcaaga taaaactaat ttaatgcaga taaaccaaat taaaatagat ggtagtcaag
     4201 tttatgcagt atttgaaagt aaaattgata ctgtaaaaat aggagattat gaaaataata
     4261 caagagctat acaagtgttg gacaatatac aaaacttcat tgaaaatgga acaaaatatg
     4321 attatataac atcaaataaa gtcagataca atgttaataa aatatttcag atgccagcta
     4381 aatagtgagt gtgcatttta gtttaataac taaagcccac tttcttttgt agtatgaaag
     4441 agggctttac atatttgtaa atatcgaagt acacttgttt tatcaataaa ttatttaata
     4501 aggtataatg gaccaattaa acaataaaaa ttctcctaaa acagcaagta t
//
LOCUS       DAEPLF010000245         4545 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEPLF010000245 DAEPLF010000000
VERSION     DAEPLF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.246"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            142..2151
                     /locus_tag="KR314_003248"
     CDS             142..2151
                     /locus_tag="KR314_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0329606.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEEIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQSFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQGVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYSITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            2395..3141
                     /locus_tag="KR314_003249"
     CDS             2395..3141
                     /locus_tag="KR314_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBH0329607.1"
                     /translation="MKKLVKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            3182..4501
                     /locus_tag="KR314_003250"
     CDS             3182..4501
                     /locus_tag="KR314_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0329608.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYADKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
BASE COUNT         1811 a          437 c          854 g         1443 t
ORIGIN      
        1 tattgcaact atgatattta gggacataaa actaataaag gaatatgtta gtatactaaa
       61 tgtacaattt tttgtgctat taaagtgaaa atcagatgta tattataaaa gagtagagta
      121 tattttaaaa ggagtaatca aatgaaaaca atagcagtag taactgatgg gttgtctaaa
      181 ttagaatgtt tcttaaataa aaatctagaa atgatattta aagataaagt aatgataaaa
      241 aattattatt taaataaaat aacagagaag gaattaataa aagatgacat aatattggta
      301 atgatagatg atagagtatt aaaaataaaa aaatatgtag aagatgtctc aaagataata
      361 actataaata ggagcataaa gcaaaaggat atatataagt tattttcatt gccagaagaa
      421 atagatgtat tagtagtaaa cgataataag catactgtat tggaaacaat atctaattta
      481 tacaatatag gaataaacca tttgaatttt ataccataca atcccaatga agaatataga
      541 aatattaaga tagctataac gccaggagag tcaagtttag ttccaaaata tatagaagaa
      601 gttattgatt taggccatag gtatattgat agttcaacgt ttattcaaat aatagcaaaa
      661 ctcaaactgg atgataaaga aattactgag aggctaatta agtatacaga tgaaataatt
      721 agtttatatt caggaataaa tactaaatac aaagaactta ctataaaagc agatgaatta
      781 aattctataa taaatttatc aaacttaggt atggcaatgg tatcagataa gggaaacata
      841 attatttgca ataatagttt aagggatata ctagatattc aaagtaatat tatagggaaa
      901 aatatagatg aattagagaa tgagaatatt aaaaagattt ttagtttgag taaggcacat
      961 gatgaagtta taaaatttaa taataagtac ttaaacgtaa ataagtataa tatagagagt
     1021 tttggaaggg ttactgggta ttatttttgt attcaagaaa taacttatat aagaaaattg
     1081 gagcaaaacc tatcaaagaa gcttagagaa aagggacaag ttgctagata tacttttgat
     1141 gatataaaaa cagtttcaac gaagatggaa agaactaaga aattaggtat gaaaatagct
     1201 gaatctgatt atacaattct tataacggga gaaagtggaa caggaaaaga acttatggct
     1261 cagtctattc ataatgcatc accaagacat agtcagtcat ttatagcgat aaactgtgca
     1321 gctatgccag aaaatttact tgaaagtgaa ctttttggat atgaagaagg tgcttttaca
     1381 ggagctttaa aaggaggtaa aaaaggatta tttgaacagg ctaacaatgg aactatattt
     1441 ttggatgaaa taggagacat gcctatatac ttacaaagca aattacttag agtattacaa
     1501 gagaatcaag taatgagggt tggaggagaa aatgtaatag atatagatgt tagagtaatt
     1561 gcggctacaa ataaaaattt attagaaatg ataaaaggag atagatttag agcagattta
     1621 tactatagga taaatgtatt gcccataaat ataccacctt taagagagcg aaaagaagat
     1681 atagaagtta tgttaaagca ttttatgaaa aggaagagag caattagtca aggtgttcaa
     1741 gacattatta atgcatatga atggccaggt aatataagag aattaaaaaa tactgctatg
     1801 tatattaaca ttatgagttc tgaggatgtt attttaataa atgatttacc tcataactta
     1861 ttaaatataa ctgaagattt tagtaaagaa ataagcatat taaaggatag aacatctata
     1921 gagaaagtaa agttagtaat ggagtgttta aaaaatgcca ataatcttaa taaaaatata
     1981 ggtagaaatg gtattgttaa ttcaataaat gaattaggct acagtataac agaaggtgag
     2041 gtaaggggaa tacttaatat gttaaaagag ctggatttga taatatgtga atctggtaga
     2101 aagggaagtg aattaaatag aaggggaaaa aatatattaa acagtattta ataggttgat
     2161 aaaaaggaaa atctaaatgc ctattttacc tattagttgt acttaaaaca atttaaaaaa
     2221 gtcaaactaa acaggattaa aataaaatat aagtaaataa agttatttat attacttgta
     2281 ttcaaaaaaa gtatttctaa aatgaattat aatgttcaaa taaataaaat cttggtatgc
     2341 tacttgctct ttaagagaag tagcattttt tattgttcac aaataagtta aggggtgaag
     2401 aaattggtta aaaagaaata tccaataata ggaatatcag gtaatttact aattgatgaa
     2461 ggaggaatgt ttcctggcta tgaaagggct tatgtaaata atgattatat acaatcagta
     2521 gttatgtgta aagctatacc atatattgta ccgattgtat atgatgatga gattataaag
     2581 gagcaggtat ctaatataga tgcgcttata ctatctgggg gacaagatgt aaatccactc
     2641 atatggaaag aagaacctca taataagttg ggggcaatat caccaaagag agatagtttt
     2701 gatatgaaat tattaaaaca tgctttagat atgaaaaaac cagttttagg tatatgtaga
     2761 ggagaacaaa ttataaatgt aactgaagga ggtagtttgt atcaagattt atctttaatt
     2821 gaaggtgctt atattaaaca caatcagcaa catctatcta atgtacctac acatacagta
     2881 cagataaagg aggggacaaa attatatgaa atattaggag aaaaagaagt tttagttaat
     2941 agttttcatc acttagttgt caacaaagta gcaccaggat atattgtttc agctacatct
     3001 aaagacggtt taattgaagc tatagaaaag gaaggaagtg agtttgtaat agggatacaa
     3061 tggcatccag aaatgatgac tagagattat gataatatga aaaaaatatt tatggcaata
     3121 gttaaagaag catctaaata aaaaatataa atatattaag ttggctttgg ggggaataac
     3181 aatggaaaaa ggcattaagt taggtttttg gagtattgta ctattaggaa tcaatgcaat
     3241 aatagggtca ggtatattcg ggttacctgg tgatgcttat acagatatag gtccagcaag
     3301 tatattagta ttagtttttt gtatgctttt agctgtttct attgctttat gttttgctga
     3361 agcaggtagt tggtttgata ctgatggagg gccttatctt tatgcaaaag aggcatttgg
     3421 agattttgtt ggatttgaag ttggatttat gaagtggata gttagtatga ttgcttgggc
     3481 tactatggca aatttttttg ctgtaacact atcatctgta tggccacaag cagcagaacc
     3541 acttattaaa aatataataa ttggtatatt agttgtagga cttgggatta taaattttat
     3601 gggaatgaaa caatctaagc acttaaataa tatcatgact ataggaaaat tattaccaat
     3661 agttttattt atagctgtag gattattctt tataaagggt tctaatttta ccccatttgt
     3721 aataatacaa aagggtcaaa gtgcgtcatc agcttttgta gcagtatcta taactttatt
     3781 ttatgctttt acaggatttg aatctttagc tgttgcagca aaagatatgg agaaccctaa
     3841 gaaaaatgta cctaaagcat tggtaatggt aatgtttgta gtatcagtaa tatatatgct
     3901 tatactggga ataagtatag gggtgttggg taatggcttg gcagggtcag ctactcctgt
     3961 tgcagatgct gccattaaga tgctaggtcc tataggtggg tatataatta caataggaac
     4021 tatagtatct gttggaggta taaatattgc atcatccata tttacaccaa ggtctgctgc
     4081 tgctttggtt gaacaaggat taatgcctaa gtctataaga aaaactaata aaaatggagc
     4141 tccttatata gcaattatag tatctgtcat aggaacttta ctaatagcat ggtcgggttc
     4201 attcacaaca ttatctcaaa taagtgttgt atctagattt attcaatata ttcctacatg
     4261 tttagctgta ctaatattga gaaagaagta tgctgataaa gatgtaaatt ttagaatacc
     4321 tggaggagca attataccaa tatttgcagt tattataagt atattattac tgataaaagc
     4381 aggtattgat gaacctatga agatagtttg gggattaggt gggatgataa ttgtagttcc
     4441 tatatatttc tatatgacta aagtatattc aaagaaatat aatgatgtag aagtcaaata
     4501 attattgtaa tctatagata ttctataaat gttattttga attta
//
LOCUS       DAEPLF010000246         4504 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEPLF010000246 DAEPLF010000000
VERSION     DAEPLF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.247"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(237..848)
                     /locus_tag="KR314_003251"
     CDS             complement(237..848)
                     /locus_tag="KR314_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH0329609.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
     gene            complement(940..1929)
                     /locus_tag="KR314_003252"
     CDS             complement(940..1929)
                     /locus_tag="KR314_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBH0329610.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NRESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            complement(1996..2859)
                     /locus_tag="KR314_003253"
     CDS             complement(1996..2859)
                     /locus_tag="KR314_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBH0329611.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            complement(2965..3561)
                     /locus_tag="KR314_003254"
     CDS             complement(2965..3561)
                     /locus_tag="KR314_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBH0329612.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGLFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            complement(3601..4404)
                     /locus_tag="KR314_003255"
     CDS             complement(3601..4404)
                     /locus_tag="KR314_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBH0329613.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKRVIFKSFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
BASE COUNT         1420 a          812 c          438 g         1834 t
ORIGIN      
        1 attgtacttt atttagtaaa caaattattt aataaaaaaa ttacttaata cagtaaatta
       61 attggtaaga taaattattt attattagta aagtaaatta tttactattt atcaagataa
      121 attatttata aaataaatca tttataaaat ataatttata ctttgaaaat aaaataaact
      181 atcttccaaa cattttatta tatgcttaaa agatagtttt gtgtctttaa aataaattaa
      241 attgtatata ttccttcaca taacttttgt atattactag gtggttgcca tttaccatct
      301 tttttagtat atgtaatcat agttgtaact tccttagttg gtttcttatt gtaaaggtct
      361 ataatcattt ttgtcatatc atcttgaact tctttttctt ttttatcgta attcttttca
      421 tcagttacat aagattttac taaagtgttc gcttcactca ttactttact agtatctaaa
      481 gctttactta caactttcaa agttatctta tccttactag aagattccac tgttactgtc
      541 ttttcaacac cttgaattaa tttaacaatt gtttcgtata tcttttcagc attttcttta
      601 ccataaagag gtgtcaattg agcaatcatt gaaaccttgt ccttgctgtt ataagtttga
      661 atctgtttat attcagcttc tgttaatgca tcagaaattc ctttttcatc atacttatac
      721 atttctaatg cagctaaatt agcttcaaaa ttcgacttta ctttttcttc tgcattagaa
      781 caccctatag ttagaactaa aactagagcc ataatcgtag acaatattaa cttttttacc
      841 attttcatac aaacatcctc cctcaattaa aataataaaa aatttattta tcaacattat
      901 aacataaatt tgtctaacta tagaattatt ttgaatattt taccacttcc agtctattcc
      961 aataccgttt tcttttgctt tatcgtatat tctttttgct gttacgacat cttgtacacc
     1021 tatcccaaca gttttaaata caattatctc atcatcatct tctcttccag gaatagttcc
     1081 atttataacg tttcctaaat caccactaaa atcatcttta cttatcttac cttcttttaa
     1141 tggaattaat atatctcctg actcagataa aactgcatct gttgattcaa aaaatatctt
     1201 agacgctcta gttaaacagt ttgggcacaa ctcttgcata tctggcatat atgaacctac
     1261 tgcacttatt aaagctcctt ttttcactaa gtttccatta agtactggtt gttttgatgg
     1321 agtagctaaa actatcacat ctgcgttatc tatggcttca tcagatgatt caactgcaac
     1381 tattttagta ttatatttac ttaattttaa gttcatttct tctgcaaatt tttctctact
     1441 ttccctgttt ctactaaaaa ctcttacctc atccaaattt ctagcttcta tcatggcttc
     1501 aagttgtggc tcaccttgtc ctcctagacc tatcaaagct ccaatcttag aatctttttt
     1561 agccaataca tctatagctg ctccagaggc tgctccagta cgaatctgag ttacataagt
     1621 accatccaat actgcactaa cttctccagt ttctccatct aataatagta ctgttgctgg
     1681 aattactggt ttgccttttt gagcattttt aggaaataca gatacaattt ttattccccc
     1741 acagcctaat tcttctacgt atcctggcat aaataacata ctagcatctt gacttggaac
     1801 agatatattg gttctcaaag gatttacact ctttccttca caataaattc taaaagcttc
     1861 cttatcagct tcaattgcat ctctcattgt aaatactttt tttatgtcgt ctttttttaa
     1921 aagtagcatt tttatacccc caattttaat aagtttctat gctaaatatt atagcataac
     1981 atttaattta atcttctatt tcaaatcaaa taaatctaca tactctggtt ttagcataga
     2041 acttatcaac gctttattta ttgaaaattc tggtattccc gctgcatatg gagttatatc
     2101 atataaatca aaatatacaa ctagattctc atcttgcaag taaaagtttt gattctcttt
     2161 tataccttta aaattatata ttccgatatt ttcttcatcc tttttttcaa gttcagctat
     2221 ttgtctttta atttcttcat ctataacttc tttatattta gtattatcct taaataagtc
     2281 cattaattct aaaaattttc cagttctcat atccacatta tatgctatat cttgataaaa
     2341 tccatgtgct ccaccactat aagtataata tctcactttt atactaagag cagaatcagt
     2401 attcttttta agttcaaagt ctacatttgc aacaaactta tttttctcgt caggattatc
     2461 cttaagatac tgctttgctt ctttatatga tttattataa aattcaaata tgtcattttt
     2521 tatcttatca tttgtagacc tttcaacaat tttatttgaa tttgttatta caggcatgtt
     2581 tatagtactt tctaaatact catcattttt cactattgtt ttacttccta ttgtacttac
     2641 tgataaagtt tgtttactat ttgtattacc ctttggtaat gactggttac tcataacagt
     2701 cactgcttcc ttattactat ttaaattaat tgaagcatct ttccttaaat tattatattt
     2761 atagtcatgt ttaattgcca aaacagccat tatagtcaat gttaaaacaa atacatatct
     2821 taaaacttta gcttttgtat cgactttaaa aaatttcata ttatcatttt cctcctaaat
     2881 ataaatctct cttattatat ttggagtttc tagggttttt tatccatata atttaataaa
     2941 agattaattt ttatttataa aaaattaatc tttttgtttt aaaatattaa tatgtcttat
     3001 caaacagttt gaaatttcac caagaggtaa ttgtaacata acagaacctc tatcaaaagc
     3061 taccattggc attccatata ctatacaaga attttcatcc tgtccaattg taaataaacc
     3121 attttctttc atttttaaaa gtccatttgc tccatcataa cccatacctg taagtattat
     3181 tcctattgaa tttttacctg ctacttctgc tactgaatca aacaaaacgt ctactgatgg
     3241 acagtgacca cttattcttt ctcctttttc acaagttaca taatatcctt gtttatccgt
     3301 acacaatttc atctggtaac ccccaggtgc aattaacaca tttccttgtt cgactctatc
     3361 attattctta gcttctctta cattcataat acactgcttg tcaagccgtt gtgcatacat
     3421 ataagtaaat atagctggca tatgttgaac tacaacaatc cctggtgttg attttggtag
     3481 gtcttttata atttcaagta ttgcttctgt accacctaca gatgcgccta tagcaataac
     3541 catattatta aaacgttcca cattaccgcc tccaaaaaat catatatcaa aaataccttt
     3601 ttatcgttta tatatagcag gttttacata tttgtattta gtactacttc tgttgattac
     3661 ttctgcatgt cctacaaaca aaaaactatc ttttttggta gcattataaa atttatttat
     3721 aagttcttct cttgttttta aatcaaaata aatcataaca tttctacaaa aaatcaaatc
     3781 aaaatgttga tatgagaaat cattcattaa attaaatgac ttaaaaatta cacgttttct
     3841 aatcttatca caaattttaa atgtaccatc cttgttgtct ataaaatact tcttcttcca
     3901 taactcagga acattgttaa catttgattc tatgtatata cctcttttag ctttttcaag
     3961 tgagttagtt gaaatatctg tggctatgat tttaatgttc cacaactctt tattatatcc
     4021 aaaataatca tccaaagtca tagctattga atacgcttct tctccactag aacatcctgc
     4081 actccatata tttatgtttc ttattttatt attttcttcc caaaaaggaa gtatactatt
     4141 ctgtataaac tcaaaatgtt gaggttctct cataaaaaat gtgtggttag tactcaatct
     4201 atttattagg tttatagctt catttcctgt cttatcttta aataatagat tcatatattg
     4261 actaaaagaa ctaagttttt tctctatcat tgtattgtat aagcgacctt caattaacgc
     4321 tcttttttta cttaaatcaa taccatattc tttttttaca tggtttacaa gaactataaa
     4381 ttcttcatct gtgagtttta tcatgcaaaa acttcctcct gctaatttat cagaactaac
     4441 tatatgctat taatcaaaaa ctttcgaata tctaaaatta atatagaatt atcttcaact
     4501 ttcg
//
LOCUS       DAEPLF010000247         4474 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEPLF010000247 DAEPLF010000000
VERSION     DAEPLF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.248"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            359..1063
                     /locus_tag="KR314_003256"
     CDS             359..1063
                     /locus_tag="KR314_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0329614.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            1065..2552
                     /locus_tag="KR314_003257"
     CDS             1065..2552
                     /locus_tag="KR314_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0329615.1"
                     /translation="MNNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVFSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            2757..4370
                     /locus_tag="KR314_003258"
     CDS             2757..4370
                     /locus_tag="KR314_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0329616.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYEILEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNIHSQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQASFGNKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDSSVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
BASE COUNT         1778 a          395 c          749 g         1552 t
ORIGIN      
        1 ttaaaaaata tcaattttga tataaactga ataaatacag ttgaatatta aattattatt
       61 ttatcaaaaa tatgcaaagt actacacctt caaatatcac ctaaagtaat taaaaggcaa
      121 ttaaagtgaa gtagttcaag tatttctaaa atgagtttaa taaattctat aataatttat
      181 tttttgcctt aaaattgcct taaaatccaa acaattttaa aactaatgta ctataaaaag
      241 ctggtataat ttaaatatag taaaaaataa gatttatctt atctattggg atttaattat
      301 ttaatcaata tatttatacc atttaattta aataaaaagt ctattaagga gtgagcttat
      361 gagtaagtta atttatatag cagatgatga agataatata agaaatttgg taaagacatt
      421 cttaaaaaat gaaggtcatg atgttatgga ttttaaaacg ggtgatgaac ttttagaaca
      481 atttaatata aaggagtgtg acttagtaat actagatata atgatgcctg gctcaagtgg
      541 ttttgaagta tgtacaaaac ttcgtgagaa aagtacagtc ccaataatca tgcttactgc
      601 aagagataca gatatagatt atattacagg gataacctta ggtagtgatg attattttac
      661 aaaaccattt agcccaatgt ctttggttat gagagtaaaa tctatattta gaaggataga
      721 gtttgaaaag aaacaaaatt atgataaata tagtaactcg atagatatgg aattaaaatt
      781 tggtgatgta ataataaata aaaagaataa aatcgttaca agtaaaaatg taaatataga
      841 ccttacacca aatgaatata atctattgac ttatttgttt gaaaatatag atagagcagt
      901 ttccagagat gaattattaa acaaaatatg gggatacgat atagaagttg agacaagggc
      961 tgctgatgat acagtgaaga gattacgtaa aaaaatttta gatacaaata ttttaataga
     1021 aactgtatgg gggtttggat ttaggttgaa ggagaaaagt taagatgaat aatggaaatg
     1081 acaagaatag aattggaaag attaaatcca aatttaaatt aaagttctca aagaaaaata
     1141 taagcataaa aactaggatt ttgttgagca tactttgttt gataattata gtatttatga
     1201 taatattagt atcttttaat gtatttcttg atacatatat aaaaacaact gcgaatgaag
     1261 aattgacgaa gtctacagaa atagtagaac atatggatag taattttaga cctattaagc
     1321 caccacaaga tgatggaaag cttcctccca aagtattatc taattttatg agaaatgtac
     1381 aagataaagt taggatggca gaaacacaat cagatgctga tgctatggta gttgattcta
     1441 agtataacct gatatttcca acaagagagg atgatttctt aaaaaatgtt gatgagatgg
     1501 agctgattag ggagtgtatt caaaatgaaa aattagattt aaattcagat gaaaatacac
     1561 ggatatctac cagaaataag gattattata tttcaactgt aaaaattact agtatatatg
     1621 gtgaacaaga caaatatctt atacttttta tagacatatc aaaaactcta aatctagcac
     1681 aaaagataaa tattgtatta atatctgtaa tgtgttttgc tggaatatta gcaatattta
     1741 cagctgtgat tttatctgag aaaattgcta aaccgataaa agagttgtgt gagtttgcaa
     1801 aaaaaatggg tcaaggagat ttcaaaagaa cctactttga cttttcagat aaagaattag
     1861 tagagctctc aactgttatg aataagagtg ctgagtatct ggataagtat gataatgaac
     1921 aaaaaatatt ttttcaaaat gcatctcatg aattaaggac accactcatg tctattaaag
     1981 gttatgcaga agctataaaa tataatgtta tagatagtaa acatgcaagt gatataattc
     2041 ttgaagaaag tgataggtta agtgatatgg ttgaagattt attatacatt tctaaaatag
     2101 ataatataac taaagatttt gaacttgtag aatgtgattt aagagaagta ttttcaaatt
     2161 gtggagctag gcaaaatgta agagctataa ataaggggat aaaatttatt tatgaatttg
     2221 atgaagaaat ggttttattt gaatgtgatg aaaaaaacat ttccaaagca tttatgaatc
     2281 ttatagaaaa tgctttgaga tacgctaaga gtgaaataaa aatagtgtgt aagtataatc
     2341 aaaaaaatat agttgtgata atagaagatg atggaattgg aatcaagaaa gaagatttac
     2401 ctcatgtttt tgaaagattc tataagggag tagggggaaa tcatggaatt ggattatcaa
     2461 tagttaaatc tattgtaaat aagcatggtg ggcgtatata cgtagaaaat ggcaaaaaag
     2521 gagctaagtt tactatatta tttaaattgt aaaaaaatta gagaaaatat aaagatgttt
     2581 gaagttgttt tttatatata tctgttgtat actgttgaaa aaagaacaat attaagctga
     2641 catatattac aataattgac atttaaagta aatgcttaaa tatagatacg aaaggaaaag
     2701 taaaatctga agtgtattta ggtttaagta agcttcttat tttataaaaa atatgtatga
     2761 aagaacagct gatttttaat attacaaatt tagtaacggt tgtatttgaa gcttttgtca
     2821 tccatatgtt tttatcagac tttttaggtc agaaagaagg gtatataaaa gttgtaagat
     2881 atgctaaact gggttttata atatgtttag gattctgtaa tttaataact ttaaacccta
     2941 aaatcacaat gcctttaatt tttatcctta ttttttcaac atcattttta tataaaggaa
     3001 atttaaagac acgattgttt actactgttt tattaagtat attctttata ctgtcagaaa
     3061 ttgtagttac aagtattttt gttttatttg taaaagaagg ctttgaaata atgcttgaaa
     3121 ataatagtat aagagtattg gcgacaattt tatctaaaat tgtctttttg ttgacttgta
     3181 aaattatttg tctatttaaa aaagatgtac atttagatat gcccataaag tattggctcc
     3241 ctttattttt aatacctata tttagtttat ttctgtctgt ttctatcttt gatgtaagta
     3301 aattttttag tcttgaaagt ttaaaatttc taagtttaat atctagtgtt ggcattttat
     3361 atattaattt tattgtattt tatttattta aatttattat agataaaact aaactatcta
     3421 tgaaatatga aatacttgaa aaagaaataa ttcataagga agaattaaga cttagtaatg
     3481 agtgttataa aattattgtt gaacagactg attctgtcgt tttcgaatgg aatatcaaag
     3541 aaaataaatc atttgtatcg caggcttgga ctgaaaaatt tggatataac aatgcatgta
     3601 agaatatttt taaagaaatt aaagataaag acttagttca ttctgaagat aaagctatat
     3661 ttgaaggttt tcttgaaagt attaaaaaaa agaatataca cagtcaagca gtatatcgtt
     3721 taaaaaaatc taatggtgaa tatatttggt gtagaacatc aattacaagc atttataatg
     3781 atgaaaatga acttttaaga gttgtaggag taattgtaga cgtagatagt gatataaaaa
     3841 aatatgaaga gttaagaact agagcagaaa gtgactcatt gaccaatata tacaataaag
     3901 gtacatttga aaaattagtg gaagaaacta tagttatgaa tacaggtgat aaaaaagatg
     3961 cattattcat tattgattta gatgatttta aagaaataaa tgataatttt ggtcatccat
     4021 ttggtgattt tgtactaaaa acatttgctg ataagataca agctagtttt ggtaataaag
     4081 acttagtcgg aagaattggt ggagatgaat ttgttgtata tatgcaagat tatgttacag
     4141 aagtaaattt gcataaaaaa gctaaagaat taaatagagt actttcggat aattacactg
     4201 atttgagttt tagttttgat tcttctgtca gtataggtat tgcaaggtat cctcaagatg
     4261 gaacttcatt ttttgaattg tttaaaaatg cagatagagc actttatagc ataaaagcat
     4321 caggtaaaaa ttcatattgt ttatttgaag aagaactata tgtacagtga atttgaattt
     4381 agattttatg tgagagtaag atttatatat acttgtggct gtctgattta taaaatatgt
     4441 tactttgtat atttttatat gtttcagtaa ggtt
//
LOCUS       DAEPLF010000248         4444 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEPLF010000248 DAEPLF010000000
VERSION     DAEPLF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.249"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(192..1199)
                     /locus_tag="KR314_003259"
     CDS             complement(192..1199)
                     /locus_tag="KR314_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBH0329617.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNSEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISNLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            1427..2311
                     /locus_tag="KR314_003260"
     CDS             1427..2311
                     /locus_tag="KR314_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0329618.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDKKNKIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            2513..4063
                     /locus_tag="KR314_003261"
     CDS             2513..4063
                     /locus_tag="KR314_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0329619.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKEKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            4092..>4444
                     /locus_tag="KR314_003262"
     CDS             4092..>4444
                     /locus_tag="KR314_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HBH0329620.1"
                     /translation="MKKFLNVLFSVCISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSEDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVFLVGIILSVKN"
BASE COUNT         1729 a          494 c          727 g         1494 t
ORIGIN      
        1 aataaattca aaataatagt ttataaataa aatttttgga aataatatta atataatttt
       61 aataaaaatt tatattttat actatgtaaa atatctaata tatttggtct gtaggttaaa
      121 taaaagtacc tttagaccaa atatattatt aataacttaa taacatctta aaacttattt
      181 atttatatta tttacaacat ccatttaata aatacaccat aacctacagc tgggtcattt
      241 tcaattgcat gtgacactgc tccgattatt ttattatctt gaactattgg agtaccactc
      301 ataccttgta caataccacc agtttgagct aaaagctttt tatctgtaat tttaatttta
      361 aaacttttac tttctggatg tttttggttt tctatatcta taatttcaat attatatttt
      421 ttacacttat taaagttatc ttgtaaaatt atttgagctt ttcctaattt aacttcatct
      481 aaagatgcta ctttatattt ttttaaattg gaaatatcaa agttattaat ttgacctctt
      541 attccaaaat cagtattttc tgtaaagcta ccaataatat tttctcgttt tgcatttata
      601 catccaacac tacctttata agatttttct atattaaggt gcttagttgt agaaattgaa
      661 cctgttttaa taggtatttt tcttgcatta ccaacactta ttggatgacc tacagctcca
      721 aactctgatg tatctggatt gatatacgtt aaagtagcaa atccagaaag taaattgtta
      781 aaactaattt tttctaatat gtgtttggta gttttcattg ttataagttg tccacctcta
      841 tttaataaaa tagatacttg tttttcttct ggtaaagcat ttaaggtatt ggcaaagtca
      901 ctatatccat caacttcaga attatttacc tttactatag catcacccaa tctaaatgga
      961 gatgcttctc caggatttct aactatgata ttttttaatt ctgcgtcaat ttgaagtacg
     1021 tttccaattg gtattaaata ttcaggttca tttttttgta aattttgtgc ataaattaaa
     1081 ttatttgaga aaaaatataa taaaaataaa aatgttaaaa caattgtaaa ttttgactta
     1141 gctaagttgg tttttttact taaagtatta tttttaaaat gaaaattttt aagttgcata
     1201 aaacacctct ttcttattaa attattttaa taatagaatt gaagttttct tgctgcttct
     1261 atattatgtt gtgcaagaat tatgttttga tgcttggaaa actgactcaa taacacctta
     1321 gttttcataa aaataaataa aataaatagt gtatatgata aaataaatac atattcatat
     1381 aaataaaaat aaacaataac tgtctagcat aagaaaggag caaaaaatga aaaaatatag
     1441 attgttagtt acttgtataa ttgccatggg aatcataatt ataggaacat caaatccagt
     1501 tagcaatttt tacaagacta caaatttgat tagttcagga aatatcacaa atgaaaaagc
     1561 agataaagat gaaaattcaa acaaaaataa taaagataaa aatgaagaca aatctaattc
     1621 taaaagtaat aaagataagt catcagagga caaatcgtca gataaaaaaa ataaaattca
     1681 aaaaaataaa aaacttttaa tttgtataga cccagggcat caaggaaaag gagatagcaa
     1741 tttagaacca gtagctccag gttcttcctc taaaaaagcg agagtatctt caggaacaga
     1801 gggtattgct acaaaaaaac cagaatatgt tctaaactta gaagcatctc ttgttttaaa
     1861 gagtatatta gaatctaaag gttataatgt aataatgact agagagacac atgatgtaaa
     1921 tataagtaat tcagagagag caatacttgc taatgataaa aaagcagata tggttgtaag
     1981 aatacatgca gatagtttaa ataattcttc aaaaactgga gcatctatat tgattcctga
     2041 aaaagatgga aaatatacag ctcctatata tgaagagagt aataaatgtg ctgaatttat
     2101 caaacagaat atggaacaat caggtataca aatcaatgga atcgttcaaa gaggtgattt
     2161 gactggattt aactggtcaa aagtaccagc cgtattagta gaaatgggat ttatgagtaa
     2221 ctataatgaa gaccaaatga tgtcaaatcc agactaccaa agaaaaatga tgcagtgtat
     2281 agctgatgga ttggatgcgt attttaaata agatatgaat agagaaaaag ttaggttggt
     2341 attattttcc taatttttat ctctattttt tattgtacat aatgcaaaga agaagtttat
     2401 tttaggttaa attgtcgtaa atataactaa aaatattggg aatacttgat ttaagtttaa
     2461 aaagaatata ttatagtata gaatgtgtaa aatttttttg agaggtgttg aaatgctagt
     2521 agtagaaaat gtgagtcatg gatttggtgc gagaacaata ttagaaaatg tatcctttag
     2581 acttagaaaa ggtgaacata ttgcactagt tggtgctaat ggagaaggaa aatcgagttt
     2641 tttaaatata ataacaaaga agcttatgcc agatgctgga aatataaaat ggtcttcaag
     2701 agctacagta ggttacttag accaacatac ggtattaagc aaggaaaaaa ctataagaga
     2761 agtgcttaga gaggctttta agcatatgtt tgacttagag caagaaatga ttgctatgta
     2821 tgacaaaatg ggagaagcta gtgatgatga aatgagtaag cttcttgaag aaacagcaga
     2881 gatacaaact atacttgaaa atagtggttt ttatatgata gatgcaaaga tacaagaagt
     2941 tgcaaatggt cttggtcttg gagaaattgg tcttgataaa gatgtaactg acttaagtgg
     3001 agggcaaaga acaaaagtac ttcttactaa acttctactt gaaaatccaa ctattcttat
     3061 attggatgag cctacaaact atttagacga agagcatata acatggctta ctaagtacct
     3121 tcaagagtat gagaatagtt ttgtcttagt ttctcatgat attgaattta taaataatac
     3181 atgtaatgta atatatcata tggaaaatgg agagttaaat agatataaag gaaattatga
     3241 tgaatttgta agacttaatg atataaagaa acgtcaagaa gaacaagctt atgataaaca
     3301 agttgaagaa agaaaaagac tagaggattt tgtagctaga aataaagcta gagtagcaac
     3361 tagaggaatg gctaacagta gacaaaagca acttgataag atggaaatat tagaaagacc
     3421 aaaagaaaaa ataaaaccta cttttgcttt taaagatgct agagcagcta gtaaaataat
     3481 ttttgaaaca gagaatttag ttttagggta tgatgaagca ttgacaaaac cacttaattt
     3541 ccatttagaa agaggaaaaa agattgcctt aaaaggaatg aatggtattg ggaaatctac
     3601 tctattaaaa actttacttg gaataataaa accatttgaa ggaaatgtta aattaggtga
     3661 ctatttggaa gttggatatt ttgaacaaga gagttcaagg gaaaatagta atacacctat
     3721 ggatgaagtt tggagtgaat ttccagggct tacaaacttt gaagttagac aggctcttgc
     3781 aaaatgtgga cttacaaatg aacatataac gagtcaaatg agagtattaa gtggtggaga
     3841 agctgctaag gttagacttt gcaaagtaat gttaaaaaat ataaatttct tagtattaga
     3901 tgagcctaca aatcatttag atgttgaggc aaaagatgaa ttgaagaagg ctatcaaaga
     3961 gtttaaggga acagttcttt tagtatgcca tgagccagaa ttttattcag aaattgttga
     4021 tgatgtatgg aatatagaag attttaccac taaaatcgtg tagtgattta acttattgct
     4081 ggggggattt catgaaaaag tttctaaatg tacttttttc agtttgtatt agtattgtta
     4141 taatagtagg tgttattaat tttactgttg gatttaaaca actttattat tttgacattg
     4201 attacttaaa tatatcagag ttatctggtt tatctgaaga tgatataaaa ttgaactacg
     4261 attatctaat tgattataac ttaaataaga atgtgtctga gttcaagtta ccaacattaa
     4321 aatcttctcc acaagggaag atacattttg aagaagtcag gaatatattt caaaatataa
     4381 ataaattagc taaattatta ttggtagttt ttttagtagg aattatattg agtgtaaaaa
     4441 ataa
//
LOCUS       DAEPLF010000249         4415 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEPLF010000249 DAEPLF010000000
VERSION     DAEPLF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.250"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     repeat_region   43..536
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=43..71
                     /rpt_unit_seq="ctttacattccatatagttaatataaaac"
     gene            complement(1367..2239)
                     /locus_tag="KR314_003263"
     CDS             complement(1367..2239)
                     /locus_tag="KR314_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0329621.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(2633..3211)
                     /locus_tag="KR314_003264"
     CDS             complement(2633..3211)
                     /locus_tag="KR314_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH0329622.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(3269..3985)
                     /locus_tag="KR314_003265"
     CDS             complement(3269..3985)
                     /locus_tag="KR314_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBH0329623.1"
                     /translation="MRINKYIASCGIASRRKSEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            complement(4208..4399)
                     /locus_tag="KR314_003266"
     CDS             complement(4208..4399)
                     /locus_tag="KR314_003266"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329624.1"
                     /translation="MAKSLCALNFTSKSITSGFKSKASKIANKNGLKNFKNPSILDIK
                     FDGVLINLKNKIPINTTIK"
BASE COUNT         1413 a          742 c          403 g         1857 t
ORIGIN      
        1 taaaacagtt gtttttattt tttctaaaga gaaaaaacta gactttacat tccatatagt
       61 taatataaaa ctttctgtat attttagttg tgttttaata ttataacact ttacattcca
      121 tatagttaat ataaaacatg gcagtaacta agaagaaaac tatacctttt ttattcttta
      181 cattccatat agttaatata aaacgaggaa attttatact aatgactgat gctagattta
      241 actttacatt ccatatagtt aatataaaac tattataagg agcattatac gaccaaaagc
      301 caaagcagct ttacattcca tatagttaat ataaaacaag caacagcaag agtcgcgaca
      361 acattaaaaa cttcctttac attccatata gttaatataa aacctttatt tttttctcta
      421 tatcgagttc gccaaattca ctctttacat tccatatagt taatataaaa cagtccagcg
      481 ttgtgtggtc gtaacacacc aatcctactt tacattccat atagttaata taaaacccca
      541 aaataaatta agtatttcca atacttatac atatacaatt ctcctaaact tgcagtgagc
      601 gagcaatagt gcaattgata acagttatca tacactctca acgctttata tttcaactat
      661 taaaccctac tttatcacaa aaatcgctca ctgcaaaact tttatatttt tattatatca
      721 taaaactcat actataaaat aaaaatggga taattttaca aactaaaagc atctacattc
      781 ttataagtat ttattatatt ctctattcca atgattcctt tttcttttta ttgcatcctt
      841 cctatgtaat cgcactttag ctcaacaagt cttttatttt cattaacaat tttttaaact
      901 attcaaaatc atcaaatttt tcatcttata cagttaaatc aaaatacgtg ttatatctct
      961 ttatctatat acttttcttc cttccatttc tttttctctt tctacatttt cactttgtta
     1021 ctttacaatc tcaaggttat tatctgctat taaagtttta tctttccagg tttcacaaaa
     1081 ataaattgaa taagtgcaaa aataacagat acaaaaccat aactattttc ttttctataa
     1141 aaaaatatat catcaacaag aaacataata aatgaaaata actatgaata aatactctca
     1201 tttagaagaa atagacaaag atattcctta aaatttaagc aaaatattgt ttaaataaaa
     1261 aaactgactg tcagttaaat gccagtcagc tattttatat attttatcca aacatcacta
     1321 tatttgaaca ttttaataat taataatact tattaatata caaactttac tcatacacag
     1381 aatatctttt ccaaatttgc tctaaatcat caaaatgctg tttaatatct tctttttcct
     1441 tcattccaac agatatagca agggcatttg aaatactaag agctggaact agagaatcta
     1501 caaatgaagc catattactc ttaactagaa gagtgttatc tgcaagtgat gctactggcg
     1561 caaataagct atctgttaat gatattacat gggctccttt ttctttagca taattgacaa
     1621 tttgataaga tttcttagaa taacgaggga aactaatagc aactattaca tcttcttcat
     1681 ttattcttac aatctgctca aaagcatctc ccatatccat tctaattata tgaacattgt
     1741 caagtattat atctaagtaa aaacctaaat attgagctac tacaaaagaa ctcctcattc
     1801 caagtatata tatttttctc gctcttaaaa gtttgttaga agcttcttta aacgctctct
     1861 cgtcaatttc ttctagtgta gaccttatat tatctatatc actttttaaa actttgttta
     1921 gtattgcaaa atcatcggaa taatcatgtg ccatttcaac tctttgtact gtagttagtt
     1981 tattttttat gagttcttga agtgcagctt gtaatttagg atatccacta taacctaatg
     2041 catttgcaaa tcttacaact gtagactcac ttactcctac ggtttcaccc aatttacatg
     2101 cagtcataaa tgcaacttta tcataattat ttaaaatgta ttgtgctata agtttttgac
     2161 ccttactcaa tctcgtatat tgagattgta tatttgatat caaatgttta ctgtccttca
     2221 tttcatttgt ttcttccatt tttttctcct tttagttata taaactattt tgttttatta
     2281 acactgtctt tttctctaaa aattttattt tttatctcaa tttcatctta gtttttatca
     2341 tagcttctta aactcataaa attctaactt ttctgtacct tatacttcac atagtatttt
     2401 cgattaaatt tattatacca tctttccttg cattttttaa tatttatcca atatttcttt
     2461 taaatttttc aatttttact catttaaaag ttatctatct tattttgcat atttagatgt
     2521 atattttgtt ttgagaataa gctgttgtat acttattatt taaagtattt ttaataggtc
     2581 acactatata tattaactct taatagaatc ttgtcaagtt ttttagtatt tattatgctt
     2641 ttttcttttc aattaaaatc aattgtggtg ggttattgac ttgatttaaa aacttagatt
     2701 ctaaaacatt aaattcatct tgatttaaat tattgacaag gttatatatg caatcttttt
     2761 cttcttgacc tccatcatgc cctgtatata tggcaatgct tattactcca ttttctttta
     2821 acatttctaa acttaacttt atagccttta aagttgtatc tggtttggtt ataattgaat
     2881 gtttcgctct tggaagataa cccaaattaa ataaaacaca tgaaacttct tcttttatat
     2941 attgattcat tttttcatgc ccatcaaata tcaactttac tctatctgta tatccttctt
     3001 tctctagttt ctttcttgta gactttattg cttcttcttg tatatcaaaa gcatatacaa
     3061 agcccttttc tccaaccttc tcagctagat accttgtatc atacccattt cccatagtag
     3121 catcaattac tatgtcccct tcatttataa tattttctaa ataaaattta tttatatctg
     3181 taattttagt taaatacttt gcatatgcca taatactatt tctccctact attaacttta
     3241 tttcattttt tctcaattta tttttatctt attttattga ttttaaatat tttatttcat
     3301 cttctgttag gtatctatat tctccaactt tggtgtcttt taatactatt ttccccacag
     3361 aaatccttct taaatttaaa actggatgat ttatagcttt gcacattttt cttacttgtc
     3421 tatttctacc ctcatgtata gttatttcac acactgaata atttttttct ttattttctt
     3481 tagttaccct tattttggca ggtgccgttg tgtaatcttc tatttttagt cctgtttcga
     3541 attgtcttat ctcatcatca cttataattc ccttcacact tgccacatac gttttatcaa
     3601 tttcatgctt tggatgggta agcttatatg ttaaatcgcc atcattagta agtattaata
     3661 aaccactagt ttcataatct aatctcccta ttggatatac tctttccctt acatctttaa
     3721 gtatgtctat tacactgggt ctattgaatt ggtcttttac tgtagttata tatccctctg
     3781 gtttatttaa tactatatat atataatttt cacttggttt tactttttta ttatcaaatt
     3841 ctactatgtc tttttcttca tcaatattaa aagataactc ttttactaca cttccattta
     3901 cctttattct tcctccaaga attatttctt ctgattttct tcttgatgct attccacaag
     3961 aagctatata cttattaatt ctcatactta atccttcctt atcttaaatt atgtgtgttt
     4021 aatattttta tcaatgttaa ttttgctaca ttattatact atactagacc ttaattaaaa
     4081 acaagcaaaa aaattattta attttcctaa ataattgtaa tttcttttca catactatta
     4141 atatgtaata gctgactaaa cactatttta aggaagtgat tattatttta aaggaaaata
     4201 taaaatatta tttaatagtc gtgtttattg gaattttatt ttttaaattt attaatactc
     4261 catcaaactt tatatctagt attgaaggat tcttaaagtt ttttagccca tttttattag
     4321 ctattttact tgctttactt ttaaatccac ttgtgatgct ttttgaggta aaattcaaag
     4381 cacatagact tttagccatt tttttatctt atata
//
LOCUS       DAEPLF010000250         4411 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEPLF010000250 DAEPLF010000000
VERSION     DAEPLF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.251"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..1103
                     /locus_tag="KR314_003267"
     CDS             <1..1103
                     /locus_tag="KR314_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH0329625.1"
                     /translation="VDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEVV
                     ILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYII
                     SIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQI
                     EGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISELL
                     NRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEKSELII
                     RLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGLG
                     LYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            1504..1725
                     /locus_tag="KR314_003268"
     CDS             1504..1725
                     /locus_tag="KR314_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329626.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(2014..2778)
                     /locus_tag="KR314_003269"
     CDS             complement(2014..2778)
                     /locus_tag="KR314_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH0329627.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKAPTPDIVDILSEFNVNVL"
     gene            3052..3726
                     /gene="fsa"
                     /locus_tag="KR314_003270"
     CDS             3052..3726
                     /gene="fsa"
                     /locus_tag="KR314_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBH0329628.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILDEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(3848..>4411)
                     /locus_tag="KR314_003271"
     CDS             complement(3848..>4411)
                     /locus_tag="KR314_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBH0329629.1"
                     /translation="MFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIF
                     FIAAFSIGGVLKYSGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTS
                     SVVIPLLGSLHILKENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKY
                     GLTLIPLTYFIIICITFPWWKFIGLEI"
BASE COUNT         1825 a          494 c          619 g         1473 t
ORIGIN      
        1 tagtagatag ttcaattcat ttcgatacat atactttaga agaaagactt tattatatgg
       61 ctaaagacca aaatttggct attttattaa aagataacca agggaaaatt gtatatggta
      121 agaatgaagt agttatttta agatatagta agtacatgat taatagtctt gaggatgagt
      181 atagaacttc tatacctata tatactaaag atgcaaaaga tggtccatat actttagaac
      241 tcgttatgcc attacaacca attgacgaag caaacgaagt tataagaaaa ctaatgccat
      301 acatcatatc aatagcaata ttgatagcga ttattggagc atatatatat tcaattgtaa
      361 taacaaagcc tcttataaat ataatagaga gtgagagaga acaggaatat agaaggaagg
      421 attttgttgc tacaatatca catgaactaa aaactccaat aaccataata agtggtcaaa
      481 ttgaaggtat gatttatagt gtaggtaagt ataaggatag ggatacttat ctgaaaaaat
      541 catatgaatg tacacaagaa ttaaaagatt tagtaaatga aatgatagag gtttcaaaat
      601 cagaaatact tgaaaaagat ttgaaattgg tatcaataaa tataagtgaa ttattaaata
      661 gacttgtaaa gagacaagta tttttaatag aagaaaaaca tatgaaaact attttaaaaa
      721 tagaagaaaa tttggaaatt aaagcagacc aagagagaat aactaaggca atcaataata
      781 taataaataa tgccataaaa tattctcctg aaaaatcaga gcttataatt agactctatg
      841 ataaaaataa acgaataagt aaaaagactt ctaatcaaag agtggtttta gaaattgaaa
      901 atactggtgt aactatcgag aaacgttatt tagaagagat atttaatcca ttttatagaa
      961 tagaaaaatc ccgttcaaga aaaactggag gaagtggact tggactttat atagtcagtc
     1021 aaatttttaa aagtcatggg tttgattata gcataaaaaa caaagaaaac tctgtagtat
     1081 tcacagtaga atttaaaaat taattactta ttataaagaa tatataactt tcaataatag
     1141 ttcattttaa aatcacaaaa aattcataat gaaactgtat tatataaata taaagaagct
     1201 taaaaccata aaatacacca actataatga tgagatttcc ttacatcctc catataagtc
     1261 atcattatag taacccaaca attaagttac tatgaattta aaaaataaca ccgagcatat
     1321 aatctatgct tagtgttatt tttattttga aataaaaaat atttaattta ttgaaatgta
     1381 tagatttaga cagtttaaac tatatttaag acttaaaaat tataattgat attacaaata
     1441 aatatatgta ctttgggaaa aataaatata ggaagtatta tatttacaga ggaggtagtt
     1501 ttgatggata gattaaaaag agatatgaaa aatatgaaag aagatttaaa agaaggtgtt
     1561 aaaaagacag cttatgttat gaaaaataca gctgaagatt tgaaagatga tgcacaaggt
     1621 gctatggtaa acttagaaat gaaaaaagat gagatgatgg aaagatatga acaaaagaaa
     1681 ttttctaaag aattagagaa gcaaatgaag aatgaaatga aataagcttt tatttatgaa
     1741 taacaaatta ttgtattcat ataaaaagta gccagtaata ttaaatagtt tatttaagga
     1801 aatagacttt ctaaaaataa ctgtattaga tttagaaagt ctatttcaaa ttagttttat
     1861 aaatagacat aaggataaag tagatgttat agaactagat tttaaaaaat agatactaag
     1921 gataaaataa atattataga aatatgtttt taagaatagt tatcaaataa aaatttatat
     1981 ttataagaaa tcctataaaa tatataatta aacttataaa acatttacat taaattctga
     2041 taaaatatct actatatcag gagtaggcgc tttttctgta attatagaac taatatcttc
     2101 taaatgagca aacttgaaat ttccatcaaa tttaaacttt ttatcttcca taactataaa
     2161 tactttttta cttgaattta ttatagcctt cttcgtgaga ccatcttcaa tttcaaaagt
     2221 tgtaagtgaa gagttgaata catctactcc acaacttccc ataaaagtct tatcaaactt
     2281 atatttcgat ataaactcta tagttggagt accgacaaaa ccgtctaaac tgagatttaa
     2341 taaacctcca gtggaaatta tatttaaatt atttggtttt gaagtaacta catttagtat
     2401 atccaacata tttgtaacaa gtgttacttt tttattactt tcagctaaca actttgcaag
     2461 caagatgtta attgtagaga tatctaaaaa tatagtttct ctatcttcaa ttaaattaaa
     2521 tgctttttct gctattattt tctttgttgg aatattaatt tcttttctgt cttttgtatc
     2581 ctgattctct agtgtttctc tagctagtac agctccccca taaattcttc ttattgaaga
     2641 aatcttttct atatttttta aatcttttct tatacaatct tctgtaacat taaatttaga
     2701 actgagctct ttgacagtaa ctctaccttc tttttttagg atatttaata tagcttggat
     2761 tctttcttct gcaaacattt tctacctctt ttcgcaatat agtattatag taaatattat
     2821 aagataaaat acaatatatc aactatttta acatgaaata caaaattaat ttaatataaa
     2881 aagtatgttt tttctaacta tatatttaga atttagtaaa taaaaaaatt ataaaactaa
     2941 gaaggaaaag caaaacaata ataaaaaata aaaaacaata agcaaaatgt tgaaaataat
     3001 accaaatcaa tatataataa gaataaagaa agattaaagg gggatatttt aatgcaacta
     3061 atcttagata caggtaatgt ggaggagatt aaggagttat gtacttgttt accaatagat
     3121 ggtgtaacta caaatccaag tattgtttca aaagaaaaaa agaattttaa gcaacttata
     3181 aatgaaattg gtgagattat aggtgaggat atgcctatac atgctcaagt gttaagtaca
     3241 aagtatgaag aaatattaga cgaagcatta tatataagta gtttgagaaa aaatatatat
     3301 gtaaaaatac ctgtaacaca agatggttta agagctataa aagaccttca cagaaaaggt
     3361 gtaaaaataa ctgctactgc aatattcact gctcatcaag gttttttagc tgcaaaggct
     3421 ggagcaaatt atattgcacc ttatgtaaat aggcttgata atatatctgg tgatggagta
     3481 gctatggttt cagaacttat aaaaataata gatacttata aaatggatac aaaagtactt
     3541 gcagccagtt ttaaaaatgc acaacaagtt atagaattaa tgcaacatgg ggtgcatagt
     3601 gttacagtac catatgatat ttgtaaatct atgatgaatc atccattaac tgattggagt
     3661 gtagataagt ttatagagga ttgggagaat acttttggaa aaggaagtaa aacaaataat
     3721 atttagtatt atggtaaaag agtagttttg tccaatttca caagaactta tataaaaaga
     3781 tgaaaatatg aattttcttt ttaataattt cgttattatc ttaagttaat tcatattttc
     3841 atctttttta gatttctagt cctataaatt tccaccaagg gaatgttata catattatta
     3901 taaagtaggt taaaggaatc aaagttaaac cgtatttgaa aataaccttg ctattataca
     3961 gattttcacc atgaccaacc attattgtag catgatgaaa tggtaatatg tactgtatgc
     4021 ttactataat gtagacaaat agacataaag taacactatt ttcctttagt atatgtaaac
     4081 ttccaagtaa tggtattact acagatgatg tagtgacagc acttccaaga catatattca
     4141 agattattgt gagagttatc aaaaacaata ttgaaataat ttctgaagag tttggtataa
     4201 actttatcaa ataatttccc attatatcta caactccaga gtactttaac actcctccaa
     4261 ttgaaaatgc tgctataaaa aaaataagga ccttccaatt tatttctttt aaagtactaa
     4321 atccaactat ttttcttagt aataaaagaa gtacacaaat tgacattata attaatgtgt
     4381 ttatgttatg tatgaactga gttaaaaaca t
//
LOCUS       DAEPLF010000251         4402 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEPLF010000251 DAEPLF010000000
VERSION     DAEPLF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.252"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(363..512)
                     /locus_tag="KR314_003272"
     CDS             complement(363..512)
                     /locus_tag="KR314_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBH0329630.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(525..755)
                     /locus_tag="KR314_003273"
     CDS             complement(525..755)
                     /locus_tag="KR314_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBH0329631.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(1447..1668)
                     /locus_tag="KR314_003274"
     CDS             complement(1447..1668)
                     /locus_tag="KR314_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBH0329632.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            2248..2505
                     /locus_tag="KR314_003275"
     CDS             2248..2505
                     /locus_tag="KR314_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0329633.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            2954..4192
                     /locus_tag="KR314_003276"
     CDS             2954..4192
                     /locus_tag="KR314_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBH0329634.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKRNKSATKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1720 a          438 c          630 g         1614 t
ORIGIN      
        1 tactcaataa tgaaattatt ttttcaatgt tattatctag agcagaacat tttatggtta
       61 tttcaatatc atcacaatta ataccttgtt cgacatgtat tttcattaat catcacctca
      121 ttttattact gtcattagaa tagcattata tcttattgag tccaactaga tgcgttctaa
      181 atttcaaatt aagcatagtg aaattcaaaa ataaaatagt gaaattcaaa tttgaggtgg
      241 taaaattcaa aaaaatacta tggatatttg gataaattat ccaaatatcc tagtatttag
      301 catagaatat gtctaattat tttacaaata gacctatttt atgacttaca tatattgtca
      361 aattacttct taaatatcaa aatatttttg gataatgtat caatactgtt tgataaagta
      421 tttaatttac cctcaattct aaccaacaag tacatggata gagctatagg aaaaccaaca
      481 ttggttatta acagttgtaa actatcatcc ataaaattag ccccctattc tataactaag
      541 tcataaggag tggtatttgt ttccacaacc ttggcttcaa caacagaagc taggtcaaat
      601 cctccaaccg aaaaaatatt tttagatact atcaaatcca tgacagattt tatattttct
      661 tcagttaaat catcctttgg attttctata gaaaaagaaa cttgtttatc ctttacattt
      721 ttaaagctca tgactaattt tttcttaatt tccatataat tacctccgat ataataattt
      781 atattttaat tgtttgcttg ttaatattgg tattttaatt gtcaatatta gtgttttgct
      841 tgttagtatt aatatttcaa ttgtcaatat taatgtttta cttgttagta ttagtatttc
      901 aattgtcaat attagtgttt tgcttgttag tattaatatt tcaattgtca atattagtgt
      961 tttgcttgtt agtattaata tttcaattgt caatattagt gttttgcttg ttagtattag
     1021 tatttcaatt gtcaatatta gtgttttgct tgttagtatt agtatttcaa ttgtcaatat
     1081 tagtgttttg tttgttagta tcagtgtttc aattgtcaat attagtgttt tgtttgttag
     1141 tatcagtgtt tcaattgtca atattagtat tttaattgtt gactctagtt attgctgata
     1201 tagaaaatat ttaatttatt tcaattaatt agattaaagt agggtagcat ttaaaatagc
     1261 taacatgttt aagttgaaat acgtttaata tattaatttt tcaaagcatt aatatattaa
     1321 agttataacc aaaattgaag ttataatctg ccaaatctat agcgtattaa gtgcgttata
     1381 taacttaatc ttttatataa agagttaatt tttttaattt gtaaagcaga gtacgtttaa
     1441 aagatactat ttagataatg atgtactatc taattttaaa atatctacaa tagggttctc
     1501 ttgaagactt gctattgctt ctgcaacagc ataaacatct tcatttaatg cctctgcttt
     1561 tacatttgca aatgatttac ttttaacaac tgccttaccc ttatcatcaa aaccagcatc
     1621 caattttagt tttatactag ataaattttt tgttgaaaca acttccataa aattacctcc
     1681 taaaaattta atttcaaaga tttgtcgact ttctttgtta gctataatac tatcatctaa
     1741 gataatatcg gagttaaaca gtaatttcta aagatttttt gtgtaaattt ttataggaaa
     1801 aattttactt ttctgatgat gaaaaagttt acaagttatt tttgaagata tgttcttagt
     1861 gttttgaaat agtatttgta tatattcatg cttagttgag atagttttgt atggattttc
     1921 atatgtgtag agttaaatgt taggtaccaa agtttatgtt aaattaaatg ttaaagctta
     1981 tgttaaaaat tttattaaat ttaatctgta aataaaaaag ttaaaagtcc aatattaaca
     2041 ttttactttt aaaccattaa aatctttatt aaagctaaca aagttataag ttatgagaaa
     2101 ttttattaaa gttaatacaa aaattaattt taaagctaag aaatttttta ttagattcaa
     2161 taaaactaat aaatatagtt tagtcaataa tgtattaaat aaaaactttg ttaagtctat
     2221 aaaaatatct taaaggaggt gtaacttatg aagataaaag tggaggatat accagttcaa
     2281 tttcatgcaa tggtagaaat aatcggaatt gataagttca tagaggttgc caagttgtat
     2341 ggaggaacaa atacttatat acctacgtat aaggggcttt ttagatatgc tagaaatagg
     2401 gatatagtaa aacaatttaa tggagtcaac cataatgaat tggctattag atataatatg
     2461 tgtgtaagca atattaagcg aatactcaat gaaaataatg tttaaacata atgaaaatag
     2521 ccttgttaaa tgtaagagta aagacaaaga agatgtttta taaagttttt ttagacaaat
     2581 taactaaatt tactacttat taatttaaat acattgtata ttctataaaa aaatttgtta
     2641 aatactggag atatattgag aatacaaaga aaatggtcga aaatatttgt tggctaaaaa
     2701 atagataaaa tgatacaatt gcttgtataa aggggaaaaa taaataaata aactatataa
     2761 ataaagaaat agcaattaat atgacataat atatttataa aattaagaat ataaatagat
     2821 attttaaggg attagttgtt aaattaggaa atattaaaaa aatatatata tttatattta
     2881 aactattttg ttatttatat aaaaatactt atctagttac tagcaattat tagtagaaat
     2941 gggggaaata gagatgaata agggatttta caaattaaat tcagtggtat tgaactttga
     3001 tattaagaat gatgttaaca agataagatt taatcattat ataatgagaa aagaaaacat
     3061 acaaagactt caaggaactc tgccaaacgg acaattttat atgacagtga gaagagctgc
     3121 tacagattta caactttcta tttctactat aagtaggctt gtaaaagagt ttttggattt
     3181 agggataata agattagtgt ctaagggtgt aaaaggtaat cgttctgtat atagttacgt
     3241 aagtatagag tttaatggaa gtttatttga tgatagcaaa tatatggaac ttgactgtgt
     3301 atgtaatgat gaatttattg ataatagtgg catagagttt gattgtgata gcaaagataa
     3361 ttcttttaat ggagatataa atgtagaaac tgtgcattgc attaatgagt atttctctga
     3421 tgatgatgta gagcataatt ataacaattt ccataataaa gatactataa aggataaagc
     3481 catttctaat attagcacta tcaaaaaata tattgatgaa tcttttgata caaataaatc
     3541 agaaaattct aacttacaac aggagtcaat aaatttcaac tttaatgata catgcaatga
     3601 aatagataaa agaacgaata atagtataga aaataatcaa aataaaaggc ctatgagatt
     3661 gagtgacatc agtgttttaa gtgataaagg tatgacaaat aaaaagaatt actttaatca
     3721 taatcatggt atacaaaaaa ggaataaatc tgcaacaaag aaaaaagaat ccttaaataa
     3781 atttaataaa aaaaatatac agcaaagcat tataaatatg ctaaataaaa aatcaggtaa
     3841 gaattttaaa atcgattctc caataagcat aaaacttatc aatgaaaggc taaaggaggg
     3901 ctatgtctta gaagatttct ataaagttat tgaggtaaaa gtggctaagt ggaaaaatac
     3961 cataatggaa atgtatataa gaccagaaac cttgtttagc tataagtttg aatcctatgt
     4021 aaatgaaaac atatctatga aaagtcaaag taatgttcat ccatgtaaag aaggcaatag
     4081 taatgatagc agtaattata tacattctga ttatacaaca tatgatggca gtgtgcgtga
     4141 gagaaaaaaa tatgagaatg taagatactg gaatcctata tgtgaaattt aattagttta
     4201 agaatgtatt tatgataaat aagatattaa tagagaatag tgagctatag caaatgatat
     4261 atcaatagtt ggtagtaagt tatattaagc aagatattac tatcaggtag tgaattatag
     4321 tgaacgatat atcaataatt ggtagtaagt tatatcaaat aagatattac tatcgagtaa
     4381 tgaattatag tgaacgatat at
//
LOCUS       DAEPLF010000252         4396 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEPLF010000252 DAEPLF010000000
VERSION     DAEPLF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.253"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..476
                     /locus_tag="KR314_003277"
     CDS             <1..476
                     /locus_tag="KR314_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH0329635.1"
                     /translation="KADNIIEFFRTTHDIFYDYLLGRILDSLIIGIIAFLGFQFVIRI
                     ENALFLASIIFLGNIIPYFGPFIGAIPPIAMTILYSPQKTIWVIAFLFILQQLDGNFI
                     EPKVMGNQVGIGAIWVISAILVGQSLFGFIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(631..1290)
                     /locus_tag="KR314_003278"
     CDS             complement(631..1290)
                     /locus_tag="KR314_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329636.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KYSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(1507..2799)
                     /gene="lysA"
                     /locus_tag="KR314_003279"
     CDS             complement(1507..2799)
                     /gene="lysA"
                     /locus_tag="KR314_003279"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBH0329637.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(2817..4010)
                     /locus_tag="KR314_003280"
     CDS             complement(2817..4010)
                     /locus_tag="KR314_003280"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBH0329638.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
BASE COUNT         1333 a          801 c          467 g         1795 t
ORIGIN      
        1 acaaggcaga taacattata gaatttttta ggactactca tgatattttc tatgattatt
       61 tacttggtag aatattagac tctcttatta ttggtataat tgcattttta ggatttcaat
      121 ttgttatcag aatagaaaat gctttatttt tagcttcaat aatattctta ggtaatataa
      181 ttccctattt tggtcctttt attggtgcaa tccctccaat agccatgact atattatata
      241 gtccacaaaa aactatatgg gtcattgcat ttctatttat attgcaacaa ttagatggaa
      301 attttataga accaaaagtt atgggtaatc aagttggtat aggagctata tgggtaatat
      361 ctgctatttt ggtgggacaa tctctatttg gtttcatagg agtttttctt tcagtaccaa
      421 tagctgctgt tgtaaaaact tatgttgata aatatataga taatcgttta caatagctta
      481 tctctactgt gactttaatt tttagtttaa aatttaactg ttatttatta aattatttct
      541 caaaaaatat ctcttagtaa gaaaaaattg ctaagagata ttttttataa tatataattt
      601 gttcattaaa tttatgcata tattttatac ttattgatta tttgagtttt gagaatttcc
      661 accactatct gaaccaccag tagtatcttg tggagttggt gctggtgttg gattactatt
      721 atttggcttt tcttgatttt gagaagaact gttattattg tcagacttat tactatctga
      781 ttttttatca ttatttttag aactatattt attattttta ctacttccac tataagaaga
      841 tttccttgca tctgattcaa ctctatacct atcttctcca gccgtatctt ttgccttttt
      901 ctttctttgt tcctctgctt cgttttgttc tttttctaat ttagttaact cttctttctt
      961 tttagttgcc tcttctttaa taacagagga accatttttg attttctcta taagtttaag
     1021 ttccttgatt gcaccctcat aattttttaa ttcttcaaac tccgtcatat ttgacattct
     1081 catagcttgc atatacatag ctcttgcatt ctcattagta ctatcttctt ccagtgcaga
     1141 ggataatgat tccatagctt ttttatagtt atgtttttct aaatatgttt ttccttgctc
     1201 tactaaattt atttcatctt ttttattaaa tgaacatcct acaagaaata tagataatat
     1261 cgatattaat aaaattactc ttcctctcat taaatccctc cttgtcatat actttaaatt
     1321 ttatcataat tataaagttt ttaaaatatt ttatatttat tacaagaaat ttgttagtaa
     1381 aacaaaaaat aagcagatat gctacgaacg atacaataaa ccaaaaatta atttagagat
     1441 acatatccta gcaaatctgc tcataataaa ctttaatatt taatttttat tatatacttt
     1501 tccattttat atctctaaag ctaataagtc ctcatagctt tgtctcttac aaattatctt
     1561 atcatttcca tcaaatactg atactactgc tgctcttggt attttattat aatttgaaga
     1621 cattgaatat ccatatgcac cagttgatgt tgttataagt atatctccac ttttaatatc
     1681 cattatgttt atatctccaa ttaatatatc tccactctca caacattttc cagctattgt
     1741 tacatgattc atagcctcat gattaatttt attcacaata ctacactcat aactagcttg
     1801 gtacaacgaa ggtcttacat tatctgtcat acctccatct acacttacat aaaccctaac
     1861 atcttttata tcttttatag aacctactgt atacaatgta cttcctgcat ttgcaactat
     1921 tgatcttcct ggttctatta ccaatgtagg tacttctatt ccaagctctt tacatgaagt
     1981 ttctgcttta gtgattatgg tttcacaaaa ctcttttata gttttaggtt tatctccttc
     2041 gctataataa acgccaactc ctccacctaa gtcaatttct gttagttgta tgttgaattt
     2101 ttcttttatt tcttttacca agttcatcat tatatccact gtatctatgt atggttctac
     2161 atcaaatatt tgtgaaccta tatgagcgtg aagacctaca agtttaatat ttttatattc
     2221 acttaatttt tctattgctc taaataaatc tccatttact aaagcaaatc caaatttaga
     2281 atctatctgt ccagttttta tataatcatg tgtatgagct tctataccag gagttattct
     2341 aaagtatatc tcttgagttt tacctttatc ttcacataat gcttcaagca aatctaattc
     2401 ataaaaatta tccactacaa atttacctac acctaaatct aatcccatgt ttatttcttc
     2461 tttagtctta ttgtttccat gaaagaaaac tttttccata ggaaaatttg atttataagc
     2521 tgtatataac tcgcctcctg aaactacatc taaacacatt tgttcttcat ttataagatt
     2581 acacatgtaa agaggtaaaa aagctttccc tgcataagca accttatttt ttccctctct
     2641 agccttaaaa tattctatat attctctaca attttgtcta actaaagctt catcaaatac
     2701 ataaagtgga gtttgatatt ttttactaag ttctaaacaa ctcactcctc caatatataa
     2761 ttcatcatta tttacagtca tagttccatg aagtttcata atagcctccc caaattttat
     2821 atattaaata cttctgctat tttttctata gctacttgct tatcatcaga atttatagca
     2881 caagttatac gaatctctga tgtagttata agttttatca ttataccatt ttcattgaat
     2941 atcttaaata ctttagcagc aactccagaa gtatttttca taccaagacc tactaaagaa
     3001 aatttagtga tattattatc aatcactaca tgttcttcat ctgtatattt agatactatc
     3061 tttttacatt ctcccaactc ttcttttgga actgtaaatg atacacttat cttatctaaa
     3121 ataggtgcag tttggcttat catatctaca cttataccta tagttgctat atcttcaaat
     3181 aagctagata tattttcagc tttaaaatct tttattgtta tagaactgtc atcatcacta
     3241 gtagctaatc ctgttattac tttatcctct aatttcatgt cttttccccc tcttatatat
     3301 gttccttttt ctgtaccaca tgtacgtccg acatagattt caacaccata tttttgtgca
     3361 agctctatac ttcttgaatg cattacttgt gctcctaaac tcgctaactc aagcatttct
     3421 tcatattcaa tctcatcaag tttactagcc tttgaatatt ttcttgggtc tgtaaaataa
     3481 attccatcaa catctgtata tatctcacat tttccattta atttaaccgc aagagctaca
     3541 gcagacgtat ctgaaccacc tcttccaaga gtggttacat caccatcttc gttgattccc
     3601 tgaaatccag ttactatcac gacatttcct tcatctaaac tatttttaat tctgtttacg
     3661 tttatatcat caatttgaga ctttccatga agaccactcg tttttatatt taactgataa
     3721 gcattataac ttatagcttt acatccaagt gcatttaatg ctattgataa taaagatgct
     3781 gaaatcattt caccagtaga cattaaagca tctaattccc tcttactagg ttcattactc
     3841 aattcctttg ctaaagttat atattcatca gtggattttc ccatggcaga cacaactatt
     3901 accatttgtg gattttcttt tcttcgttct attatatttt ctgctattga ttttattttt
     3961 tcagtatctg ctactgaact tccaccatat ttttgaacaa ttatactcat gctttctccc
     4021 cctcaagcta aattttgata aataaaaacg cccaaaggta tactttggac gtgtaaattt
     4081 catagaccaa gtaaccttgt agctcaccac tttaaaagtg acagccttat gcatattctg
     4141 cataagtcca gaaaatttgt ccgtcaacaa aatttcttcg gctatatacc ctttcatcaa
     4201 ttatcaatgc ttaccagctc ctattgaata ctattgaata cagctacctc taccctaggc
     4261 tcattatttt taattaattt attaatatag ttttatcata ttctatataa tattgcaaca
     4321 attattttac aaaatgtggc aaatcgatag ttttcttttc taaagttagc ttatatacaa
     4381 ttaaatagtt tattta
//
LOCUS       DAEPLF010000253         4392 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEPLF010000253 DAEPLF010000000
VERSION     DAEPLF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.254"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            85..732
                     /locus_tag="KR314_003281"
     CDS             85..732
                     /locus_tag="KR314_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0329639.1"
                     /translation="MNILIADDEISMLKILCAYFKKENFNVFTAKNGEEALDIFYENK
                     IDLAILDWMMPIIDGIEVCKEIKENSNTRVLMLTAKSQSEDEIEALNIGADEYIKKPF
                     DPRVLIIRAKKLINYKNTINIKDLKIDFGSSKVFKGDKELLLTKKELELIRCLINNKG
                     MVLSREKLLDLVWGLDYEGDFRTVDTHIRRLRVKIGEDIIKTYRGLGYSLEDFND"
     gene            725..2119
                     /locus_tag="KR314_003282"
     CDS             725..2119
                     /locus_tag="KR314_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0329640.1"
                     /translation="MTKLNKKLSISISIVVVVVYIISIIINSMFIHRYYLHEKRNILD
                     NVENEIKDKDIDKLKSDITLIEEKNNIDIVYIELDKNHKDKGNIDKINQDLLNAFWKK
                     GLSLNKFWIEENSLNNIDKKSINKIYNQGKTKYSLLVKFIKKDNYLFAISIPIEHSEE
                     TIGIVNRFNIIMGIFSVVIITILTFILSNRVIKPMERLKFLSKDISELNFRTEDIKTN
                     DEIEELAYSINIMSEKLEKAHNELNKRNENLKTFISDASHEMKTPIALIKAYAIGMKD
                     GLDDGTYTDTIIEQAENMTNTINSLLYWAKYEKKERSLCLVDLKERLYKSLKNYELLI
                     ERGSISVKCSVDDKELIISSDEDSIDMVFNNLISNAIKYTNNNEIEINLFEENERIIL
                     SIKNGIDYNMGDDIENMWKPFYVLEKSRSKELSGTGLGLTIVRTILEENNFRYRVEIC
                     DGIIEFYIIFIKVI"
     gene            2544..>4392
                     /locus_tag="KR314_003283"
     CDS             2544..>4392
                     /locus_tag="KR314_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0329641.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAITVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSSKVDKVVLKNTITAANKLYNEAIEGTNVGEYKVGSKAIYKTAIDKAQAI
                     LDKSGVTQKEVNDAVTALNTATDTFKAGKVVAVDKTALQDAVTAATALHA"
BASE COUNT         1913 a          427 c          769 g         1283 t
ORIGIN      
        1 aaacttcctg taacatatat tttatataat taaagtaatg gatataatat gaaatataat
       61 aatgaatttt agggatgtga ataaatgaac atattaattg ctgatgatga aattagtatg
      121 cttaagatac tttgtgcata ttttaaaaaa gaaaacttca atgtgtttac tgcaaaaaat
      181 ggggaagagg ctttagatat tttttatgaa aataagatag acttagctat attagactgg
      241 atgatgccaa taattgatgg aatagaggtt tgtaaagaga taaaggaaaa tagtaataca
      301 agggtactta tgttgactgc caaaagtcaa agtgaagatg aaatagaagc actcaacatt
      361 ggagcagatg aatatataaa aaaaccattt gatcctagag ttttaataat aagagctaag
      421 aaactaataa attataaaaa tacaattaat attaaagatt taaaaataga ttttgggtca
      481 agtaaagttt ttaaaggtga taaggaatta ttattgacta agaaagaatt agaattaatt
      541 cgttgtttaa tcaataataa aggaatggtt ttatctaggg aaaaactttt agatttagta
      601 tggggattgg attatgaggg agattttaga acagtagata ctcatataag aagattaagg
      661 gtaaaaatag gagaagatat aataaaaact tatagaggat tagggtatag tctggaggat
      721 tttaatgact aaactaaaca aaaaattgtc cataagtata tctatagtag ttgtagttgt
      781 ttatataata tctataatta taaatagtat gtttattcat agatattatt tacatgaaaa
      841 aagaaatata ttggataatg ttgaaaatga aattaaagat aaagacatag ataaattaaa
      901 atcagacatt actttaattg aagaaaaaaa taatatagat atcgtatata tagaattaga
      961 taaaaatcat aaagataaag gtaatataga taaaataaat caagatttat taaatgcatt
     1021 ttggaaaaaa ggtttaagtt taaataagtt ttggattgaa gaaaatagtt taaataatat
     1081 tgataagaaa agtataaata agatatacaa tcaaggtaaa acaaaatata gtttgcttgt
     1141 aaaatttatc aagaaggata attatttgtt tgctatatct atacctatag aacattctga
     1201 agaaactatt ggcattgtaa atagatttaa tataataatg ggaatttttt cagtagttat
     1261 aataactata cttacattta tattatcaaa cagagttata aaacctatgg aaaggttaaa
     1321 gtttttatct aaagatatat ctgaattaaa ttttaggact gaagatataa aaacgaacga
     1381 tgaaatagaa gagctagctt acagcataaa cataatgagc gaaaaacttg aaaaagcaca
     1441 taatgaattg aataaaagaa atgagaatct aaaaactttt atatcagatg catctcatga
     1501 aatgaaaact ccaatagcac ttataaaagc atatgcaata ggtatgaaag atggtttgga
     1561 tgatgggact tatacagata caataattga acaagcagaa aatatgacca atacaataaa
     1621 ttctctttta tattgggcaa aatatgagaa gaaggagaga agtctgtgtc tggtagattt
     1681 aaaggaaagg ttatataaaa gtttaaaaaa ctatgagctg ttgatagaaa gaggtagtat
     1741 aagtgttaaa tgcagtgtag atgataaaga acttataata agttcggatg aagatagtat
     1801 agatatggta tttaataatt tgattagtaa tgctataaag tatactaata ataatgaaat
     1861 agagattaat ctttttgaag agaatgagag aattatatta tccataaaaa atggtataga
     1921 ttacaatatg ggagatgata tagaaaatat gtggaagcct ttttatgttt tagagaagtc
     1981 gagaagtaag gagctttctg gtactggtct tggtcttacc atagttagaa ctatacttga
     2041 agaaaataat tttagataca gggttgaaat ttgcgatgga attatagaat tttatattat
     2101 ctttataaaa gtaatttgaa ttaaaatgta aatataaaga ataattaaaa aaatatcgca
     2161 attatttgtt tttcatatgg ataaaattgg ttatttaata tataattaca agtataaata
     2221 ataaaattta tttgtaaaaa gagtagcact tttaattcta aaggctactt tttttataca
     2281 aaaccatgtt ttttataaca attcattaac ataaaaatca aacttacctt aaaatataaa
     2341 tttacaaatt tttcaatcaa taagaaaatt tctctattcc ttacataatg actgtaatgt
     2401 aacaaaataa ttacaaatta attatgaaat cattatagtt agaaacctct ttaaaattag
     2461 atattaagta tcctttagaa ttagaacggg aacaaagtta aaaaattaag taatatcaaa
     2521 tggacaaggg ggaagaaaat aaaatgaaaa taccaaagaa gattgctgca atgcttacag
     2581 ttacaatgat agctggaagt tcaacagcag gcatagctag tgcacaaact gtggcaacaa
     2641 atttaacagg gcaagaaaga tatgaaactg ctgttaaaat aagtcaagat ggatggaaaa
     2701 atgctgatga ggtagtaatc gtaaacgact catctatagc agatgctcta tctgcaacac
     2761 catttgcaaa agcaaaaaat gctccaatac ttttaacaag taaagataaa ttaaatgata
     2821 agacgaaagc agaaatacaa agattaaaag ctaaaaaagt atacttaata ggtggaactt
     2881 ctgtattaag tacaaatata gaaaaagaaa taaaagattt aaaaatatct tttgagagaa
     2941 tctctggagc tgagagatat caaacatcct tagaattagc aaagaaatta gatgctataa
     3001 gtgatgttaa gaaaatagct gtagttaatg gtgaaaaagg acttgcagat gctgtaagtg
     3061 taggagcacc agctgcacaa aacaatatgc cagtaatact tgctgattct aaaaatggaa
     3121 cagcagttgc agacaaattt ataaaagatg ctggaataac tcaatcttat gtagtaggtg
     3181 gagagagttc aatttctgaa gctgttaaaa ataaactacc aaatagtaca agactaggtg
     3241 gaacagatag aaatgataca aatgcaaaag taataaaaga attttataaa aagacagatt
     3301 taaaaaatgc ttatgtaact aaagatggta tgaataaaca agaccaactt atagatgcac
     3361 tagcagtagg tgtattaggt gctaaaaatc aatcaccagt agttttagtt ggaaagaact
     3421 tatcagcttc acaaaaatct ctagttaact ctaagagttt tgataagata actaaagttg
     3481 gtgggaatgg taatgagaca gcatttaatg aaatgaagtc attacaagaa gtaaaaacag
     3541 ttgaagctaa aacaatttct gaattaaaat cagctataga taaagctact gctaatgatg
     3601 ttataaactt taagccaact agtgaggtta aagaagcatt tacaatacaa actgataagg
     3661 cgataacagt aaacttaaat ggtacatata caaagactgt gactatcaac atgcctaatg
     3721 gtgatgtaaa taactatgca aaagtggatg atgtagttat agatgatgta aaagatggaa
     3781 cttttgttaa ctacggaaaa ataactaact taaaggttaa tgataaaaat ggagccaaga
     3841 tagaaaataa ttctaaaggt gagattggta gtttaacagt agcatctgga gcaagtcaag
     3901 taaaagtgac taatggtggt aagataacta cagttacaaa taattctaaa ggaactacta
     3961 tagataataa aggtacaata agtagtgtta agggagataa ttctccaaca ataagtggaa
     4021 atagtccaag ttctaactct tcaggaggaa gttcatcaag tggaggttct tcacatggag
     4081 gaggaagttc ttcatcaaaa gtagataaag tagttttgaa aaatacaata acagcagcaa
     4141 ataaattgta taatgaagca atagagggaa caaatgtagg tgaatataaa gtagggtcaa
     4201 aagcaatata taaaacagca atagacaaag cacaggcaat attagacaaa tctggtgtaa
     4261 cacaaaaaga agtaaatgat gcagtaacag cattaaacac agcaacagac acatttaaag
     4321 caggaaaagt agtagcagta gataaaacag cattacaaga tgcagtaaca gcagcaacag
     4381 cattacatgc ta
//
LOCUS       DAEPLF010000254         4339 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEPLF010000254 DAEPLF010000000
VERSION     DAEPLF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.255"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            249..2561
                     /locus_tag="KR314_003284"
     CDS             249..2561
                     /locus_tag="KR314_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0329642.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKENNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDTTSNKVNYISDELD
                     TTSNKVNYVSDELDTTSNKVNYVSDELGMALHKVNCVSDELDMTSNKVNYVLDELDIT
                     SNKTDYISNELYNLSNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIKFKVCF
                     DMFMTDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFME
                     LAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNLVAWEDKKFET
                     PSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKK
                     SIAQAYINQRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHG
                     NPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            2649..2984
                     /gene="spoIIAA"
                     /locus_tag="KR314_003285"
     CDS             2649..2984
                     /gene="spoIIAA"
                     /locus_tag="KR314_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBH0329643.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            2998..3426
                     /locus_tag="KR314_003286"
     CDS             2998..3426
                     /locus_tag="KR314_003286"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBH0329644.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            3449..4213
                     /gene="sigF"
                     /locus_tag="KR314_003287"
     CDS             3449..4213
                     /gene="sigF"
                     /locus_tag="KR314_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBH0329645.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
BASE COUNT         1792 a          418 c          817 g         1312 t
ORIGIN      
        1 taataacaat tcaaaataaa taacttatgt aagaaccgta ggaactacgg ggatagcctg
       61 tggagaatta gtaagacata ttttagtatg caaaattctg tgaagcagga acactgtgac
      121 tttagtcatg ggaggttcag aaaatatagc tatgttttat atactgtagt tttgaacata
      181 gaaagtttaa taaattatag tatatattaa tttttaataa ggtaagaaag tagaaagaag
      241 gatattttat ggatataata aaaaaattaa gtcatggatg tacattagat tgtcatgatt
      301 gttgtaaatt caatgtttat actaaagaaa ataatgtagt aaaaattgaa ggagataaaa
      361 atcatcctta tacaaaaggt tttatatgca aaaaagggat ggctcattta gatagattaa
      421 atcataaaga tagaataaag actcctatgc ttaaagttga tggagtatgg aaagagattt
      481 cttttgataa ggctatagaa ataatggcag aaaagcttac atactataaa gaaaagtata
      541 catcaaagtc tgtcatgcat tatgaccaat atggaagtgg gtctgtgcta aaatacatag
      601 gagatatatt ttttaatttt tatggaggtg ttagcagaca taagggtggt ccctgttgga
      661 gtgcagggat gcatgctcaa aaatatgatt ttggagtagc taagagtcat gctatagaag
      721 atatgttaaa tagtaaaagt atatttgtgt ggggaaaaaa tccagcatat actacaattc
      781 atactatgca aataattaaa aaagctaaag aaaaagggat aaagatagta gtgattgacc
      841 ccatatatac aaaaacagct caaatagcag ataagtatgt acaggtaaat cctggaacag
      901 atggagcttt agctatagct atggcaaaaa ttatagtaga agataaatta tatgatgaag
      961 aatatataaa ctcgtatgtt attggttttg aagaatataa aaaatattta tcttcattgg
     1021 aattaagttt tttaattgat gaatgtggtg ttaaagagaa tgatataaga gagttagtgg
     1081 atttatatac taacaaatat tcaagtataa atgttggata tgggcttcaa aaatataaaa
     1141 atggtggaaa cacaataaga gcgatagatg ctttaggtgc tataactggt caaataggat
     1201 ttagtggtgg aggggtaaac tatgctaata aagtttatcc aagtgttata aattctgacc
     1261 cttacaatag ccaatcatat ggtgaagata gggagtttta tgtaagtaat atcagtaaat
     1321 ttatagaaga atctttaaaa aatacatcaa ataaagtaaa ttatgcatca gatgaacttg
     1381 atacaacatc aaataaagta aattacatat cagatgaact tgatacaaca tcaaataaag
     1441 taaattatgt atcagatgaa cttgatacaa catcaaataa agtaaactat gtatcagatg
     1501 aacttggtat ggcattacat aaagtaaatt gtgtatcaga tgaacttgat atgacatcaa
     1561 ataaagtaaa ttatgtatta gatgaacttg atataacatc aaataaaaca gattatatat
     1621 caaatgaatt gtataattta tctaataagt caatcaaaga taatattcct ataaagatgg
     1681 ctgtgataac taaaagtaat atgctgaatc aactgccaga tttagtagaa ttggagaggg
     1741 tgttttcaaa aattaaattt aaagtatgtt ttgatatgtt tatgactgat acagctactt
     1801 tatgtgacat gtttataccg tgtacaaata cactagagag tgaagatata atttatagct
     1861 caatgaccaa tccatatata acctataatg aaagagctgt aaagccagca cataaattga
     1921 tggatgaata ttatttcttt atggagctag ctaaaaaaat gggattaaat gattatcctt
     1981 ttgtagaaaa gagaacttat ttagaaaaag tgattgaacc acttaagcgt tttgacaaaa
     2041 atctggatat agaaaaactt aaaaacaatt actttactat acataacctt gttgcttggg
     2101 aagataagaa gtttgagact ccatctggaa aatacgagct ttattcagaa agtataaaaa
     2161 acttgggaat aagtccaaca cctgtatata taagcaataa atataaagaa atagaagata
     2221 aaaatatttc ttttagatta ttaactaatc atcatgcaga tactttattt agtcagcatt
     2281 ttatggacaa aaaatctata gctcaagcat atataaatca gagaatggct aaaaaagtag
     2341 gtattgagga caaagatatt gtgatattga ggtcaaaaaa ggcaaaaata aatgtacaaa
     2401 taaatataga tgatggagta ggtaattata tagtaaaaat gtatgttggt tggtggaaga
     2461 aacatggaaa tcctaatagt ttaacagaca ctggtatatc agattttgga ggtcaggtaa
     2521 catataatga aagtatggta gaaataatac gacaaaacta gttaaatttc attgaaggaa
     2581 taaaaatata attatagaat tgattaaaag ttactatttt tacaaaacta ttgaaggagg
     2641 gatttttgat ggtaaattat tccttagaac ataagaatct atacatagaa tttatgtgct
     2701 cagaactaga ccaccatgta gccaatgaaa taagggaaga aatagataac ttattaagtg
     2761 taaatcaagt aaaaaatgtt gtatttaatt ttgaaaatat taattttatg gattcttcag
     2821 gtataggcgt catcataggt agatataaaa agatttctaa tgaaggggga cgagtttcag
     2881 taattaatat aagttcacga gttaagaaga tttttgattt atctgggtta aataaaatta
     2941 ttggcattta tgatacgtat gaagaagctc taagttcttt gtagggagga gtatgtgatg
     3001 aataatatta tggaagttaa attttcggca atatctgaaa atgaaagttt agcaagagtt
     3061 atagtagcat cctttgcagc taagttagac ccaacagtag atgagttagt agatataaaa
     3121 actgctgtct ctgaggctgt tacgaatgct ataatacatg gatatgaaga agacagttct
     3181 aagtttgttt tcttaagatg tgagatagaa ggaaatacta taaagattat agttgaagat
     3241 gaagggtatg gaatagaaaa tgtggagaag gcaatggaac cactttatac atcaaaacca
     3301 gaacttgata gatcaggaat gggattcaca gtaatgaaaa gctttatgga cgatgtagag
     3361 gtaagttctg taaaagataa tgggactaga attgaaatga ctaaaaaaat aaatgtgcct
     3421 aaataaagtc caaaagtgag gttttattat ggaagtaact gttgccagag aagaaaaaaa
     3481 acctcttctt agtcatgaag agactctaga acttattgaa aaagttcaaa atggagatga
     3541 agaggcaaaa gaaatattaa tatcaagcaa tttaggtcta gttaggagcg ttgtatctag
     3601 atttttaaat ataggatatg atagagaaga tttatttcag ttaggttcta taggcttaat
     3661 aaaatcaata tataaatttg acccaaaatt taatgtaaaa ttctctactt atgcagtacc
     3721 aatgatttta ggtgaaataa agagatactt aagagatgat ggtatgataa aagtatcaag
     3781 gtcgttaaag caaatagctg taaaggcaaa gatggaaagt gaagctttaa ctaaaaaatt
     3841 aggtagagag ccaagtatag aagaattagc taaagcaata gatgtagaaa aagaagattt
     3901 agtaatggct atggaagcta atttcaatgt tgagtatttg caaggagtta ttcatgaaga
     3961 ggaaggttct ccaatatgcc ttattgataa aataagcatg aaaggagaaa gtgaagaaga
     4021 aaaagtagtg gataacatat tgttaaaaga catacttgga agactggata agagagagcg
     4081 tcaaataata gttctcagat actttgaaga catgactcaa agtgaaatag gagagatgct
     4141 caatatatca caagttcaag tatctaggat agagaagaaa gtattgtcta agttaaaaga
     4201 atatatatcg tgataatact tatgatatgt agaataacaa aatataataa atatattact
     4261 aaaataaaat gtaataactt tttatgttaa gtttttaaat tattaaaaga tactaaaata
     4321 ataaaaagca ttaaaaaag
//
LOCUS       DAEPLF010000255         4314 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEPLF010000255 DAEPLF010000000
VERSION     DAEPLF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.256"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..495
                     /locus_tag="KR314_003288"
     CDS             <1..495
                     /locus_tag="KR314_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329646.1"
                     /translation="DNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYER
                     SAQDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFEN
                     SLDIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGED
                     ELNE"
     gene            488..1120
                     /locus_tag="KR314_003289"
     CDS             488..1120
                     /locus_tag="KR314_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBH0329647.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSKIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            1449..2606
                     /locus_tag="KR314_003290"
     CDS             1449..2606
                     /locus_tag="KR314_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0329648.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     FNSEEIVYFTPKGKSYHSIKNCSGLSRSKKILSGTIEESKKNDPCDICYGK"
     gene            complement(2754..3389)
                     /gene="lexA"
                     /locus_tag="KR314_003291"
     CDS             complement(2754..3389)
                     /gene="lexA"
                     /locus_tag="KR314_003291"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBH0329649.1"
                     /translation="MYLDLTEKQVLILEFIKSQITLKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(3493..>4314)
                     /locus_tag="KR314_003292"
     CDS             complement(3493..>4314)
                     /locus_tag="KR314_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0329650.1"
                     /translation="FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRN
                     SQIDNNITDGFNPIKLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKL
                     RDKAILALFVTYGLRLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKD
                     YLQNERTRDELLNDEEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHK
                     LRATAATSLIQTGFSIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
BASE COUNT         1580 a          554 c          668 g         1512 t
ORIGIN      
        1 gataatataa ataaagagat tttatttgat ataaaagaat cttcaaaaaa tatatacaat
       61 gccactgaaa gccaattcaa aattagagat tatataaaaa atataaatgc aggatttgct
      121 ctatatgaaa gaagtgctca agatttatta actaaattaa tgacccatga aaataaaatc
      181 tctcaaaatc aaaaaatcct tttagatgaa aagtttacct tagatgactc aataaaaaat
      241 ctctcaagta ttattgaaaa cttttctaat gacctacaag ttaaactgga tatgatgttt
      301 gaaaattctt tagatattca agataagtta gatgttatgt ttaataactc ttttatgaat
      361 gatgaagttc ctcttgatag tgaggaatta tttaaggatg atgttaataa tgtatttaat
      421 ccttttagtg aagaaattta tgagatagag gataaagaaa taaaggtcat aggtgaggat
      481 gagttaaatg aataataaat atagaagaac tgttaacaga gaatttgaaa agagtagctt
      541 ttggccaaca tttacagatt tattatctac tgtattgatg gttgtgattc ttatcttatt
      601 tagctcagaa agtatttcag gttctgttga acaagattta gctaagaatg tcaatgcttc
      661 tgttgaagaa acttttaaaa aaagcaaaat acctgtgaag gtagataaat ctaatgggca
      721 agtaactttt ggtgaaaaaa ccatgtttga tgtagatagt gatgttttaa aaccagaggc
      781 taaagaaatg ctaaaaatgt ttgttccaaa gtacatagag actatttata aagattatgg
      841 tgactatata tccaaaatag ttatagaagg tcatacagat gatgtgggaa gttatatata
      901 caacttagat ttatcgcaaa gaagagcata tagcgttgct aaatttatag tgggagatga
      961 aataggtgac tataaataca aagataaagt gactaagcat attattgcaa taggaaggtc
     1021 aaaagctgaa ctcatcaaaa atggtgacaa tagcgtgaat agagatgcat ctagaagagt
     1081 agaactaaaa tacgaaataa atattaatca aaataaataa ttaaattttt ataaaaaaaa
     1141 agaaggcagt tggtgtgagc tttaagcctt ctttaaggaa aaagtttatt tatttttgtt
     1201 gatacttaac atattatagt ttttagaagc agtttgcaat atattaaacg taaatggtcg
     1261 tttaaaatgc ataactggat actactgatg taaaatattt cattttatat aatataaaaa
     1321 ttatacagtg tcaattttct atattattgt actataatct attatttaaa atattatgta
     1381 aaaaaatatc ataaaaacgc atatcatgtt ataattacta gagtattatg aatatggagg
     1441 cgtagtttgt gatagaaata agtataaaaa ataaaaaaaa ttctaaaatt aaaactttga
     1501 tacttttatt gatagtatct atatcgattt atttaactgg ttgtactaca tcagataatg
     1561 gaaataatca atcaaataat aatgattctt tgtctagtag tatatcaagt gataagaatg
     1621 taaaaattca ttttattgac actggaaata gtgatgcaat attgattcaa gatggtaaaa
     1681 cttttacatt gatagatggg ggagataatg atgatgaggg attaatggta gattacctaa
     1741 acaatcaagg tgtaaaagat attaaatatc ttatagctac ccattctcat gcagaccatt
     1801 taggtggact tgattctgtt gtcaaaaact ttaatataga aaatgttttt gtatcgaatg
     1861 gttcagcaga aacaaagagt tatagggact ttataaatgc tttagcaaat aagggtttat
     1921 ccccaagtgt accgcttgaa aataataagt tctatttaga agattcttat tttgaagttc
     1981 taaatacaaa tgggggagat actactaatg aacaatctct agtccttgta tatactaatg
     2041 ggaatgataa agttttattt acaggtgatg ctgaagaagg taccgaaaaa gaaatactac
     2101 ctaagcttga gaaagtagac cttttaaaag tagctcatca tggttctcgt agtagttcat
     2161 ctcaagagtt tttagataaa gtaaatcctg aatatgcggt tcttttagta ggcaagggta
     2221 attcatatgg tcatccacat caggagacaa tgaataaatt gaaaaaaatg ggagtaaaag
     2281 tacatagaag tgatgagtgt tcagatataa tatttgaatc aacaggtgat ggagtattta
     2341 caagttgtaa agatggaagt tataataaag gtgttagaga agatggaaac tctggaaaat
     2401 caaacagtaa tacaagtaaa aatgattctt ttaaaaataa acaaaataca ttcaattcag
     2461 aagaaatagt gtattttact ccaaaaggta agagttatca ctctataaaa aattgtagtg
     2521 ggctttcaag aagtaaaaaa atattatcag gcacaattga agaaagtaag aaaaatgacc
     2581 catgtgatat atgttatgga aaataacaaa aaaacaacca atttatctat aaagcttctg
     2641 aactttaaat catttaaaaa ataaaataaa tgcataaaaa aaataagcta taatcaatag
     2701 ataatacatt tcatctatac gaatagctta tttttttaag tattttttaa tagctattta
     2761 ataactctaa atacaccagt tacaatacct acaatttcaa cttctgagtc attgagcata
     2821 attggttcca taaaatcatt ttctggctga agtcttatta aatttccttc tttaaaaaac
     2881 ctcttaactg tagcagattc accatttata agtgcaacta ctatctgaga atttaaggcc
     2941 gtattttttt tgtctactac aacgtaatct tcatctaaaa ttcctgcatt aatcatactt
     3001 tcgcctttta ctcttaatac aaaattgtta ctgcccttta ctaaactggc agggaatgga
     3061 atgtattcct ctatgttttg ttgagctaaa atgggttctc cagctgttat ttgacctact
     3121 aaaggaagct ctattatttc ttgattagct ccagaaacat catttacttt actacgttct
     3181 aaaacttcaa tagctcttgg tttagtaggg tcctttctta tgtatcccag tttttcaagt
     3241 ttatttagat gagagtgcac agttgaagta gatcttaacc ctacagcagt acatatttct
     3301 cttacagcag gtggataacc ttttaatgtg atttgagact ttataaattc caatatcaag
     3361 acttgctttt cagttagatc taaatacata acatcactca tttccaaaca tattttgttt
     3421 ttatttccat acactttata tttttaatta taacataatc catttatatc tgcaaacatt
     3481 tgttcctgat ttttaatcgt cctcaatcca ttcaaaattc ttgactatat cacgttttac
     3541 atttttctta tgagctgcat aaagctgagt agttgtgaca ttatcatggt caagtaagtt
     3601 ttgaacatca tatatagaaa atcctgtttg gattagagaa gttgcagccg ttgctcttaa
     3661 tttatgggga ctatatccat tgtctctagt agtatcaagt ggaatagatg tatatttttt
     3721 taccagtgtt cttatagcct ttgctgtaat tctcttattt tgaagagaca aaaataaagc
     3781 atctttttct tcatcattta atagttcatc tcttgttctt tcgttttgca aataatcttt
     3841 tattacatgt tcacaagtat gatttatagg cattaaaact tctttcccac gttttctata
     3901 tattttaaat tcacctcttg aaaaattaaa agaggagata ttgagttctc taagctcatt
     3961 taatctaagc ccgtatgtaa caaataatgc tagtatagct ttgtcacgta atttagtttt
     4021 tctccaatat accttttctt tctctgtaag cccttcacct gtttctactg attcaagcat
     4081 cttagcaact tcatctatct caaggcgctt aatagcatct ggctgaggtt taggaagttt
     4141 tattggattg aatccatcag ttatattatt atctatctga gagtttctgt ataagaattt
     4201 aaatagagta gagatggatg atttttttct tgctaacgct ctattgttat tttcaaaaat
     4261 caatgtgttt ttttctgtat ttttgtaata tctactgcaa taatcaccta agaa
//
LOCUS       DAEPLF010000256         4293 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEPLF010000256 DAEPLF010000000
VERSION     DAEPLF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.257"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(654..1181)
                     /locus_tag="KR314_003293"
                     /pseudo
     CDS             complement(654..1181)
                     /locus_tag="KR314_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            1465..2031
                     /locus_tag="KR314_003294"
     CDS             1465..2031
                     /locus_tag="KR314_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0329651.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKND"
     gene            complement(2222..4258)
                     /locus_tag="KR314_003295"
     CDS             complement(2222..4258)
                     /locus_tag="KR314_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBH0329652.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLKNVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
BASE COUNT         1431 a          739 c          474 g         1649 t
ORIGIN      
        1 gattccaaag atgtctcccc ataatactgg atgtctatat ttgacatatt atatgcatat
       61 cctctaccat gagtaacttc catttttcat cacctatcat aatattaaaa tatgcaatta
      121 cttaaaacaa gtattttgaa ggttttttat atttattttt aaataacata tataagaacc
      181 gtaagttctt aacttttatc atttttgagg ttgtcgttca cataagttcg ctactaccta
      241 tgcagttctc ttatgaactt cttacacttt catgtaagca cagactatat cttatccttc
      301 ggcattatcc gctaaggtct acccacttcc actaccaata acttgtagtg tacttccctc
      361 aagaggaata gtcgttgaag tttctcctat tcggagctta cctgctgatt gcccatttta
      421 aagtacttag ggtttaacct tataccatct aaccaatttt ttctactttc gtaactttca
      481 cacttaggtt tatttcatcc ttatgtttta gtttggttag ctttagaggt ttccagcaat
      541 tcaagtagta ttggataggt tctatcctat ctctacatat aggtttccct atatgctgac
      601 tattttagct actaactcat gactaaagtc acaagtgtgc atagctattt tttaatcaaa
      661 ttatttattt tattactttt agaacgtctt ccatactttt taatctcacg attgattatc
      721 agatgagcaa gatttagaat gattccttgc tttccatttg aaccacatag tataagacca
      781 gataaattat tactgtaatc cattatatat ctttgacttg caaatgaacc catgctgtgt
      841 ccaaatagat aaattggcaa atcttcattt tctttcttta taatatttgt taaagtatac
      901 atgtcctcta caagacatct aaatccttct ttttcagcta aatgtccaac attttctatt
      961 atctttgcag tttttccatg acctctatgg tcattgatat atactatata accattttta
     1021 gtaagtacct tggcaaaagt ctcatacctt tgtgcagttt cagccattcc atgtgcaatt
     1081 tggataactg cctttggttt ttttatattt tcatcttccc atttatatgt atatatatct
     1141 aaaccttcct ctcctttgaa agtaaagttt gtacacttca taattatccc tccagattga
     1201 ttttattcag tatattaatt tatatattat attacaagta ttttcaagtc aaaatctttc
     1261 taaaaaattt aagaattatt aattcctcat agaaattatc attggtacat agaagttttt
     1321 acatacaatt atacaataat ttaattaata gttacataaa aaagtaatac tttaatatat
     1381 tttgacacca tattgacatt agaattaatt catgatattc tgttttcgac aaaacaactt
     1441 ctaaaaataa aggagatata tttcatggat tattcaaatg aactaaaaga gctcttttta
     1501 atgaatcaaa catatgcaac tctttttacc cttacaaaca aaatacaaat agaaggagat
     1561 aaatattttg gtatacttac ttcaagacag tatatgacta ttttatcaat tcttcattta
     1621 ccagaggaag aaacaacatt aaataatatt gcaagaaaaa tgggtacaag taaacaaaat
     1681 ataaatagac tagttgctaa tcttgaaaaa aatggatatg ttgatgtaat tccaagtcct
     1741 catgataaga gagcaattaa tgttaaagtt acagacttag gaaaaaaggt tatggtaaca
     1801 tgtagtagga ctggaataaa ctttatggca gatgtatttc acgaatttac caaagatgaa
     1861 ttagagacct tatggagttt actaaaaaaa atgtatcgtt ttaatggtga agaacaagat
     1921 ggctttgaag aagatgctaa ttttatggaa tatgaagaaa tagataaaat taaatctgaa
     1981 gcacttgaag aatttgcaaa aagaagaaat agggtaaata aaaacgatta atactatcgc
     2041 atctataata ccactttttt atagtacaat attttatata actataatgt catttttatt
     2101 ttttaacaaa acaatcatta catagattag gtgttcttag gaactctttt tctgtttttt
     2161 gtacaaaaaa aggagatgat tagactttta ttttaaagca tctccttttt cgctattatg
     2221 attattctac tcctaaaatc atatctttta atttttgtaa atcattaatt ggttgactac
     2281 aactattact ttgacacacg taataactag tcttatcatc cttcaatcta tagttattta
     2341 aaaaacctat aatagtgttt tcttcattgt atttctttgc taaaaaagtt gcatttggta
     2401 taaaattttc acttatcaat tctttaaatg caattaaatc agaatcttcc ttaaaaatac
     2461 atataatttc cttagtagaa taaagttcaa acatcaatga aagcatataa aagctgtagc
     2521 cagttggaga actctttaca ttatccacat acagttttaa ttgtttatat gacatttctt
     2581 caagtctatt atctccagta attttcgcaa gtcttataag attgtataat tgaacagaat
     2641 ttcctgatgg aatagctcca tcatacaaat cttttggtct tgctattaaa ttttcactat
     2701 ctttaccata tatataaaat ccactttttt cataatccca aaaaaggttg atacagcttt
     2761 cattcaaatt taaagccttt tctaaatatt tcatattaaa tgttgactca taaagctcta
     2821 tatacgccca gattaaaaaa gcataatcat ctaaatatgc taaatagtcg gaacttccat
     2881 ctctatatct agctaataat ctacctgact catttacaag attattattt ataaagttaa
     2941 gacacttatt tgaatactct aaatatatgt cattttttaa agtactatat gcctttgtta
     3001 aagcaactat catcaatgca ttccatgatg taagtatctt gtcatcttta tgcaaagatg
     3061 ttctttcttt tctatattca aatacctttt tactaaggtc agctatcttt tcattatgtc
     3121 tttcatattc tttatttttt ataagattag gaatactttt accttcaaaa tttccactac
     3181 tagttatatc aaaataatta ttaaagaaag ttccatcttc ttcacccaaa acttcaatta
     3241 tctcaagagg attaaatgta taaaatttac cttcttcacc ttcactatca gcatcctgtg
     3301 ctgaataaaa cccaccttct ttatccttca tctctctaac tacataatct atagttttta
     3361 tagcaatttc tttatataac tctttctttg ttattttata tgcatctaaa aaagctatag
     3421 taagcatagc attatcatat agcatttttt caaaatgagg agccaaccac tttttatcag
     3481 tagagtatct tgaaaaccca aatccaatat gatcaaacaa gcctcctcta tacattccat
     3541 ctaaagtttt ttccaccatt tttaacacat ctttatcttt ttctatgcta taatatttca
     3601 ttaaaaacat aagattctga ggacttggaa atttaggagc attaccaaaa ccaccatact
     3661 tttcatcata aatggcctta aatactctaa ctgatgaact caacatctct tttgataaat
     3721 caccctcagt attttttaca ccaaaatcat ccttaagagc ttctattatt tcatctccag
     3781 attttattaa tatatcccta cttgtattcc atttttcaga tacattcttt aataaatcta
     3841 taactccagg tctattatac cttgaatatt ttgggaaata tgttcccgca aaaaaaggtt
     3901 ttttatctgg tgtcattatt atggtcattg gccatccacc actacctgtc atagcttggc
     3961 aaactgtcat atacacgcta tctacatcag gtctttcctc tttatctact tttattgcca
     4021 caaagtttct attcattatt tcagcaactt cttcatcttc aaaagattct ttttccataa
     4081 catgacacca atgacatgtt gaatatccta cacttaaaaa tattggttta tcctcttctt
     4141 ttgctttttt aaaagcctca tcattccaac tataccagtt tataggatta taagcatgct
     4201 gtaaaagata tggagatttt tcatttatta gattattagg ttttctatta ctgtccaaaa
     4261 taccacctca tttgttattt tattatatgt ttt
//
LOCUS       DAEPLF010000257         4252 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEPLF010000257 DAEPLF010000000
VERSION     DAEPLF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.258"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..354
                     /locus_tag="KR314_003296"
     CDS             <1..354
                     /locus_tag="KR314_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329653.1"
                     /translation="VLFFIAVGSILYFNNISAISDSTSDYKILQKMGYDNIQIKKIIK
                     KQVLPFFNIPFLLGILDCIFATLVYKTALMQNLLGNSFSQYIPVIIAIFLTSVVYLIY
                     YILTIKSCCKIVLKN"
     gene            498..2081
                     /locus_tag="KR314_003297"
     CDS             498..2081
                     /locus_tag="KR314_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0329654.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFL
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRNQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNINDNGVKNTSFKTPLDITKITFDIIKHKGIISIKEQVENLGGTISISDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            2053..2673
                     /locus_tag="KR314_003298"
     CDS             2053..2673
                     /locus_tag="KR314_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0329655.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYEEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTNMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(2861..3787)
                     /locus_tag="KR314_003299"
     CDS             complement(2861..3787)
                     /locus_tag="KR314_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBH0329656.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSENS
                     NAEIVTKNSDIFKNIEKYIDATINNNQKTMKYKHRDELFKNSNTNTYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENLDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         1516 a          577 c          490 g         1669 t
ORIGIN      
        1 gtattattct ttattgctgt tggaagtatt ttatacttta acaatatatc agcaatttca
       61 gattcaacat ctgattataa gattttacag aaaatgggtt atgataatat acaaattaaa
      121 aaaattatta aaaaacaagt attgccattt tttaatattc cgtttttact tggtatattg
      181 gactgtatat ttgctacctt ggtttataag actgctttga tgcagaattt acttggaaat
      241 tcattttcac aatacatacc tgtgattatt gcaattttct taacttctgt agtgtatttg
      301 atatattata tacttactat taaatcctgt tgtaaaattg tattaaaaaa ttaatattta
      361 ccaaatcgtc aagcttagag gtgtgacgat ttggtatact tcatttataa gtatttgata
      421 taatttaaaa ttatagattc ttaggtttga tttagtcata aaattaatat aattaactca
      481 taaggaggtg tataagattg acaaatttta atttaaaaat actattttta ggaaactaca
      541 ttctatcact tctcttcatg ggaatatcta caagcactga aacttatatg aatatccaaa
      601 gcattattcc cttaataatt tcaacattat tactcataga catcacatat ataacctctt
      661 gtaatgtccg agaaaataac attatttcat tattttgtgg attactagca ctagacagtt
      721 ggtatttact cttatcattt gtaaccacac caataggcac tattattttt aaaatattaa
      781 gcacagttat agtgtatgtc tctattaaat ttatattttt atttttgttt caaggtagtg
      841 gatacaattt ccgtaagata acagatattt tattgcttat cacctgcatt ggtgcaattg
      901 taggagcatt tatttctaac agaacgtatg cttgtatgta cggaatacag tttgttgtta
      961 gtataataag ctttttatat attatttttt atcatttgaa aagggttgtt tttgtcataa
     1021 aaagcgaaaa aaagcctata tcgatttcag tatttataac aattatagga ttccttactt
     1081 attatttttt tacactaaga atatcaaatc atattggtaa ttttggaatc tatctaactg
     1141 tattggtatt ttttatgagt attcatggta ttgcacttaa agaaaatgat agttttccta
     1201 tttcaacagt ttttaacata aaacaaaggg ctatatttct ttttataagt attttagtaa
     1261 ttagcttggt cgtactattt gccaatggga atttttctat aatattaata gctataaatg
     1321 ttttatttat gcttattttc ctctgtaaca ttattttgga gtttaacctt agagatgaaa
     1381 aaaataaagt agttaaaaat agcaagtata cttttgcatt agaaaaatta caacaagaag
     1441 aacaactaaa aacagagttt tctaattttt tacatgatga catattacag aacctgcttt
     1501 caataaaaaa catgatgagt aagtcatatc gcccagaagt tcaagatatt atatatgaaa
     1561 cacttaacaa tctaaataca tgtataagaa atcaaatgca agactatcat cctgttattt
     1621 taaaaaattt aacagtaaaa gaaaattatc agaatttaat tgaaagtgtt tcttcatcct
     1681 ttccacaaaa aaacatttgt gtttcattta agtgttctga tactttattc ttagttgaac
     1741 catatgatat attgatatat cgactgatta aagaattatt gacaaacgtg tataaacatt
     1801 caaatggtag ccatgcatgg atagctttag cactagaaaa aaatataata aaattaaata
     1861 ttaatgataa tggtgtgaaa aatacatctt ttaaaacacc tttagatata actaaaatta
     1921 cttttgatat aataaaacac aaagggatta tatcaattaa agaacaagta gaaaatctag
     1981 gaggaacgat aagtatttct gataatatac ctcatggagt ttgtgtacag attacaatcc
     2041 ctatgaaagg agatggttct tataaatatt ttgttagttg atgaccatat gctttttgct
     2101 aaaagcttgt caattgcact agaagaatac gaagaaatcg aacagtttta ttccacacag
     2161 gacatccata atctttcaga aatgattcaa gataaaaaaa tagacattgt cttaatggat
     2221 ataaacctta gaggcttaag tgaatgtgat ggcttaaaag tagcttataa tttattagag
     2281 actaattcaa ctattaaaat aataatcttg actggatacg acctcccagt ttataaacat
     2341 gaggctaaaa aaataggtat ctatggattc ctaaataaga atattagccc tgatagttta
     2401 attgattcat tattaaaagt ctatcaaggg gtaacctgtt ttccatctga aaatactgaa
     2461 gtaattattg aagatttaac taatatggag aaaagaattt tacagcttat ctgtagtggt
     2521 aaaaaaagaa aaataattgc tgatgaattg tatataagtg aaagaacatt atcaaatcat
     2581 ttacaacaca tatatgacaa gctagatgtt tcatcatcag ttgaggctat tacaaaagcc
     2641 attaaaatgg gttatatttc tccagtatat taaaaaagtg agtgtctcaa aatgaacttt
     2701 ctagctcata aggcactctc ctttgtatta aatcaaatat attttcatca tttaaacaat
     2761 taagaagagg cttctctcta ttttgagaca actccatttt aatacactat ttatatttat
     2821 atataaacat attttaaaca actaaaaagc aactaatatc tcaatacata tcttcataac
     2881 acatatcacc ataattacaa gcagataaag aactctcctc atttaaatac gactcaaatt
     2941 catacccaaa cagaattgtt ggttctatat atatttccat atctgtatat ctccatcttc
     3001 ttacctttgc ctctataacc ttgtaaaaat cttctaaact atacccttct tttaatcttt
     3061 tatctataag tgccttagtt atactcaaat cagctttaaa attttttcct gattcactat
     3121 ttaattttct tataataatt tcatgtatgc tttctggatt tttataagta gaatcttcag
     3181 tcaaatcttc atccaaattt tcaaaggtat ttttatttaa attcttttta tttaaattat
     3241 tttttaataa ttcttttttc tttgtctcat tttctgtttc atacccagta tgaattatca
     3301 aatcataatc tttgtctata ctctcttgat ttatacggct gtatgtgttg gtattactgt
     3361 tcttgaacaa ctcatctcta tgtttatatt tcattgtttt ttggttattg ttaatagttg
     3421 catcaatgta tttctctata tttttgaaaa tatcactatt ctttgttaca atttctgcat
     3481 tactattttc agaacaaaca taactatata cagaacaaca atttcccttt actcccctac
     3541 ttattaatct caatatacct aaatctataa attcatttac aagtctactt attgtagata
     3601 tagatacatc aaggtcagat gcagctttcc ttactgtcat cataaattgt cctcttggca
     3661 gagttccttt taatctttgt atattttctt ttctcattac atagtgatta aaccttattc
     3721 tatttacatc tcttttttca ttaaaatata atactgctga atttaattga taaaaactat
     3781 tactcatatt cccactcctc caatttaaat agataatttg ttgtaaagtt tttagatttt
     3841 taaataactt attaaatgat tatttttatt aaaaagtatt ctttgatatt ttcattttaa
     3901 agttttaagt ataataagtt aatagtgtag atttaacttt tttatttaaa caataaactt
     3961 aatattattt ttaatataat aattttatta aatttagtat gtaatttttt aatcttattt
     4021 tattccctct atactcttat gctaaataat aactttgata atatttccct aacttatgtt
     4081 tcaactggtt tatattgcaa tttatttaat tacagcttat ttgcataata attcgtccat
     4141 atcataagtt gtgtatttat ataaatacac aattaaaaat aaatcactat ggtatactaa
     4201 ttattacgaa gactatactt atatgcaaaa ataaatataa ggcatcttat at
//
LOCUS       DAEPLF010000258         4235 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEPLF010000258 DAEPLF010000000
VERSION     DAEPLF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.259"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(11..1201)
                     /locus_tag="KR314_003300"
     CDS             complement(11..1201)
                     /locus_tag="KR314_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH0329657.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENVENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSAGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(1461..1892)
                     /gene="dut"
                     /locus_tag="KR314_003301"
     CDS             complement(1461..1892)
                     /gene="dut"
                     /locus_tag="KR314_003301"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBH0329658.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            2005..4233
                     /locus_tag="KR314_003302"
     CDS             2005..4233
                     /locus_tag="KR314_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBH0329659.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEYEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
BASE COUNT         1502 a          676 c          585 g         1472 t
ORIGIN      
        1 aataaaacaa ttataaaact cttcttgcat ttacatatct acttgaatag tagctactat
       61 ttatattaga tattgttact ttttttgatg aacctgaaga tgcatgtatc atatctccat
      121 ttcctatgta tattccaaca tgacttacag aaccattatt tgaaccttgt gtatcaaaga
      181 atactaaatc tccacttcta aggtctcctc tatttacata tgttccatac ttactttggt
      241 ctctagatac tcttggtata ctaactcctg cagatttttt cataacgtac tgtgtgaatc
      301 cagaacagtc aaaactattt ggaccttctg ctccccatac atatggtttt ccaagtagtg
      361 tctttgcaaa acttattaca ctatcagctc cttgacttgg agaattagtc gaataagatg
      421 gaattgaagt atcaacagaa cctttttcag caaggtattg acttgacaca tatccagttt
      481 gattgccata acttactttt gtccatcctc cactttcaga aatacttcca acctcagtac
      541 cataacttaa tgtagctatt ttagaatgac ttgtacctgg tcctgttcta acgttcaatg
      601 actttgctgt tacaaccttt gtacccttta caaggtcatt tgaactgttt tcaacatttt
      661 catttgtaga gtcatttaca ctagttccta aatatttact tgaaacataa cctactctac
      721 cattagaact tatttttgac catccatttg actcagatat gtatccgact tcagtcccat
      781 atcctagtgt tgatatttta gaactacttg tacttggtcc tgttctaaaa tttaatcctt
      841 ttgctgttac aacttttgtc gacttaactg aagtatcact actttcattg ctacttccaa
      901 ggctatttat tgctacataa tttccatgca cataacctgt gtttccatta tatttaactt
      961 ttacccaaga accttctttt cctacgtact caactttatc acctttgtaa agtttcccca
     1021 ttgattcatg gtttgtacct ggtccttttc taaaatttac cgcattccct gttattgttc
     1081 tgctttctat actcacactc tctacttcag actcattagc atatgctaaa ttccccatag
     1141 taggcattat tatcgcagtt gccattatac tggctgctat tctcttttga ttaaaattca
     1201 taactttcca ctcatctcct cttttctact atctttattt cacttcaaaa tataaaagaa
     1261 ataattgaca ctattaaaat atcaattatt tcttttatat taatattatt acaaatttgt
     1321 aactatttgt ctatagtaat atttgcttac cataaattac catgtataaa ttttaatctt
     1381 caatagcctg taatttagta atatagccat cttttacctt ttttaaaaat ggctatattt
     1441 tttaactttt ttgtaacttt ttaaaatcca gttgagccaa acccaccttt tcctctttcg
     1501 gagtcactta attcttttac ttcttctatg ttaatatcat agataggttt tactatcatt
     1561 tgagcaattt tcatatgctt ttcaacctta aaatcatttt tcccatgatt tattaatata
     1621 attttaagct ctcctctgta tccttcgtca atagttcctg gtgtatttaa tacagtaaca
     1681 gaatgcttta aggcaagccc acttctaggt cttacctgcg cttctgtcat agtaggaagc
     1741 tctatacata tcccagtttt gataagttta gtctctccag gaggtattac aacctcttct
     1801 atagaataca aatccatacc agcatcacct ttgtgagcaa aattaggtat tatagcatca
     1861 tcatttaatt tttttacttt taaattgtac atcttatatc tcctttcaac tttctataaa
     1921 tattataact taaatttcga cctcttgaag ttttttattt ttttgtggta atataattat
     1981 tagtgtttta aaggaggatt ttttatgaaa cttgtacttg ctgagaaacc atctgtggca
     2041 aaaactatag catcattttt aggtgcaaag accagacaag atggttactt tgaaggaaat
     2101 gattatataa ttacttatgc agttggacac ttagtatcat tatatgatat gaaggattat
     2161 gataaagata aatactctgg ctcttggaaa atgaacaatt tcccatttgt tcctgaggat
     2221 aagtttaaat ttaagattga tagttctaaa actaaacaat tcaacactgt aaaaaagctt
     2281 ttgaatagaa aagatgtaga atatgtaata aatgccactg acaatgaccg agaaggagaa
     2341 cttatagcat ttttaatatt tttactggca aaaaataaaa aaccagtaaa aagaatcttg
     2401 gtaaatgaat ggacaccaga agatattaca agaggtataa aaaacctaaa agatgaatat
     2461 gaaatgagaa atcttcaagc tgctggatat actaggctta ttactgactg gttgattgga
     2521 ataaatttca cttctgttgc tacattgaaa tatggaaatg ggaaactatt aaacataggt
     2581 agggtaattc ttccaacagt taaattagtc tatgatagag atatggaaat actaaacttt
     2641 gttcctaaaa cttattatga aatagaagga cattttaaag ctgaagctgg tgaatataaa
     2701 ggtaaatatg ttaagggtaa agaaagtaaa tttgatacac ttgaagatgc aaataaaatt
     2761 atagcttcaa ttacatctga aactggtaaa atactagata aaaaagttac tatgtcaaag
     2821 gaatatgcac caaaactttt tagtttgaca tcacttcaag gatatataac ttctaagtat
     2881 tccaatttta catctgataa ggttttaaat gtctgtcaat ctctctatga gggtaatggt
     2941 aagggtggat acataactta ccctagaact gattccattt tcttagaaga aagtttggct
     3001 tcaaaagctt ctcaaacttt agataaatta aaggttggtc ttgaatatga aaataaaatt
     3061 aaattttcta aaactaaaag agtttttgat tcttctaaag tagactccca tagtgctata
     3121 attccaactt atataattcc aaaaagttta tctaaggatg aacaattagt ttatgatgcc
     3181 ataaaagata gatttgtagc aaactttatg cctcctgctg aatatgagaa tacagaaatt
     3241 aagactgaag tagataattg tactttctta acaaaaggta aagtgcttaa atcaaaagga
     3301 taccttgagg tatataataa ggaagaaaaa aacgatttac ttccacttgt aaataaagat
     3361 gatgttgtag atgtgttaga aataaaacca ttaactaagc aaactactcc acctaaacca
     3421 tatactgagg acaccctttt aaaggctatg aaaaattgtg gtaaaaatgt gcctgaagaa
     3481 gatacaacag tgctatctgg atattcgatt ggaacatcag ccacacgtgc tgatgtgtta
     3541 aagaagataa gtcaagtggg ctatgtaaag aaaaaaggga aatcttattc tataacagac
     3601 cttgggaaaa atttagttga aatttttcct gttaaagact tatttgatgt agattatact
     3661 ggaaaacttg aaaaaagttt aagcgatatt caaaaaggaa agtatacaag aaaagaatat
     3721 cttactaata tcattagttt tatatatcaa aatgtcaata ttataaaaca agatgcttca
     3781 aaaaatataa acacagaagc atatactttc gattcaaaaa ctaaaaaatt tactaaaaca
     3841 aaagaaaaaa aacagaaaaa agatgtaact aatactgaaa aagaagttgc tatatcaaaa
     3901 gattctaata acaaaaaaga agttaaaaat ggtgatgaaa atcaatctct tggcaaatgc
     3961 cccatttgtc aaggtgatgt actagaattt gacaaaggct ttgcttgtaa aaatcataaa
     4021 gaatgtaaat ttgttatttg gaaaaatgat aagtatttaa gtctttataa aaagaaagtt
     4081 aacaaaacaa tggtcaaaaa tattttaaaa aaaggtgaaa ctaaagtaaa atctttaact
     4141 gctaaaaacg gtaataaatt tgatgctaca ttaaaatatg ataaaaatcc agataccggc
     4201 tatttcaatt ggaaaataga gtttgacaac taaat
//
LOCUS       DAEPLF010000259         4233 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEPLF010000259 DAEPLF010000000
VERSION     DAEPLF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.260"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..918
                     /locus_tag="KR314_003303"
     CDS             <1..918
                     /locus_tag="KR314_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0329660.1"
                     /translation="LCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIE
                     INEDEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLE
                     ILKIIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNI
                     YLDLDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATT
                     EIGNMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFS
                     KIYKRVDSIAKVISICENSSFEKFVNLFN"
     gene            1085..2002
                     /gene="pfkB"
                     /locus_tag="KR314_003304"
     CDS             1085..2002
                     /gene="pfkB"
                     /locus_tag="KR314_003304"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBH0329661.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYAKRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            2039..3958
                     /locus_tag="KR314_003305"
     CDS             2039..3958
                     /locus_tag="KR314_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0329662.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         1671 a          434 c          801 g         1327 t
ORIGIN      
        1 ttatgtaaaa aaataaagtt aggtataaat caatcaccta taatggctca agatataaaa
       61 ataaaatatc catttgcttt tgaactagct aatatagcta aaaagacaat agaaaaagat
      121 ttgaagatag aaattaatga agatgaagtt gcaaacatag ctctacatat aggaggagca
      181 gttgaaagag catcttataa taataaagat aaggttttca aaactataat agtttgtact
      241 tcaggcatag gcacatcaat gttaataaaa gctaaattag agaatatatt taaggaaaaa
      301 ttagaaatac taaagattat tccttcatac ttagttgaat atataaaagt tatagatgtt
      361 gattttgtaa tatctactgt tcctattgaa gtaggagatg tgccagtaat aaatatatct
      421 ccaattttaa gtgagaaaga aattagatta attgaaaaat atatagaaac aggaaatata
      481 tacctagatt tagatataaa aagcctgttt gatagtgaat tattttttac tgatttggtg
      541 tttaaaagga aagaagaagt tatagatttt atggcaagta aacttgtaca aaagggctat
      601 atagatgagg atatgaaaaa atcttacttt gagagagaaa aaatagcaac aacagaaata
      661 gggaatatgg taactatacc acatggagct attggaaaaa tttttgataa taaaatagca
      721 attggtatat taaagaagcc tatttcttgg gaaatatcag atgttcgttt aataataatg
      781 ttagcccttg ataaagataa aatattagat tatgaagtaa tattttcaaa aatatataaa
      841 agagtagatt ctatagcaaa agttataagt atatgtgaaa atagtagttt tgaaaaattt
      901 gtaaatttat ttaattagat gggtagtatt gtatatacac ctgtaaattt ggataatata
      961 aaactaaata agataaaaac gataaatttc tcttttaatt aagagaaatt ttttgacttt
     1021 caaggatatt aatataaagt taagatataa acataaagca aataaataaa aaagaggtgg
     1081 caatatgatt tatacagtga cattaaatcc atcaatagat tatatagtta agctagatga
     1141 gctaaagaca ggaagtacaa atcgtgtaaa agaagaatac gtttatccag gaggaaaagg
     1201 tataaatgta tctcgcatac ttaaggaact tggaaatgat aatacatctt taggttttat
     1261 aagtggtttt actggagaat atataataag aacattggaa gaaaaagaat tgaagacaga
     1321 ttttataaag ataaaaagtg gtttttcaag aataaatgta aaaataaagg cgttaaagga
     1381 aactgagatt aatggacaag gtccaaatat agatgatgaa gacattgata ctttatataa
     1441 aaaacttgat aagctaaatc aagatgatat tttaatattg gcaggaagta taccatctac
     1501 tttagatgaa aaactatacg aaaatataat ggctagatta gaaaaaaaga atataaaagt
     1561 tgtagtagat gcaactaaaa atcttcttct taatgtttta aaatacaaac cttttctaat
     1621 taagcctaat aacgacgaat tagaagaact atttggtgtt aaattaaatt caattgaaga
     1681 catggtaaaa tatgctaaaa gattaaagga aatgggagct ataaatgtct tggtatcaat
     1741 gggaaaagat ggagcattgt tgattacaga ggaagatgaa gtgcttataa gtgatgtccc
     1801 taaaggaaaa gtaaaaaatt ctgtaggagc aggtgattca atggtagcag gatttatatc
     1861 tgggtatttg aatactggga aatatgatta tgctcttaaa ttgggagcag cttcaggtag
     1921 tgcaactgct ttttcatatg acttagccaa aagagaatac attgataaat tggtaaatga
     1981 gatatctgta aagcagtttt aaaatattaa aaaatatata gaaaggtaat taatatttat
     2041 gaaaattata gatttattag atgaaaagtc aattaagtta aatttaaatt caaaaaccaa
     2101 atcagaagca atagaagaat tagtagattt ggtcgcaaat agtggaaatt taaatgataa
     2161 agaaaactac aaaaaagcaa ttttggctcg tgaagaaatg agtacaacag gaattggaga
     2221 aggtgtagct atacctcatg ctaaaaattc ttcagttact aaagcttgta tagcagcagc
     2281 agtgtcaaaa gaaggtatag attatgaatc gtttgacgga agtttatcaa atctattttt
     2341 tatgatagct gcaccagacg gagctaacaa tactcatctt gaagtattat caagattatc
     2401 tacaatactt atggatgaag actttagaaa taaacttata aattcaagct ctgaaaaaga
     2461 atttaaggaa ataatagata aaaaggaaag agaaaaattc agtgaagaat atcaagatga
     2521 gaaagtggct gaaaaaaata taatagatac taaagagaat gatgctaata agtatcctaa
     2581 ggttttagca gtaacagcat gtccaactgg aatagcacat acatttatgg cagctgaaag
     2641 tttaaataaa atggcagaaa acaaaggtgt atcaataaaa gtagaaacta atggttcagc
     2701 aggtgttaaa aataagctta caaaagatga aatagaaaat gcaacatgta taatagtggc
     2761 agcagataaa aatgttgaga tggctagatt taatggcaaa aaagtcataa aaactaaggt
     2821 ggctgatggt atacacaaag cggaagaact tatagacaag gctgtaaatg gagatgctcc
     2881 aatttaccat ggaggagatg gaagtcacaa cgaatcaaat gaagaaagtg aaagtggatt
     2941 tagaaaagta tacaaacact taatgaatgg tgtatctaat atgttaccat ttgttattgg
     3001 tggagggata cttatagcga tagccttttt acttgatgat tatactatta acccttcaaa
     3061 ctttggttca aatacaccga tagcagcgtt ttttaaaggg ataggagata aagcttttgg
     3121 atttatgtta cctgtacttg ctggatatat agcatactct atatctgata gaccagcgtt
     3181 tgttgtagga tttgtaggag gtgctctagc aggtgatggt ggttcaggat ttttaggtgc
     3241 actacttgca ggttttatag caggttattt agtagaaggg cttaagaaaa tatttagtgt
     3301 actaccagct tcattggaag gaataaaacc agtattgtta taccctttac ttggaacact
     3361 tttgatggga attataatga catttttagt aatacctcca gttacagcaa ttaataatgc
     3421 aatggttgga tttttaaatg gtttaggcgg aacttcaaaa atagttttag gattagtatt
     3481 aggtggaatg atggctgtgg atatgggtgg acctgtaaat aaagcagcat atgtatttgg
     3541 agttgcatca cttgaatcag ggcagtttga aataatggca gcagtaatgg ctggtggtat
     3601 ggttccgcca cttgctatag ctcttgcaac tacatttttt aagaatagat ttactaaaga
     3661 ggaaagagat tctggtaaag ttaattatgt aatgggatta tcatttgtta ctgaaggagc
     3721 tatacctttt gcagctggag acccacttca tgttatacca gcttgtgttg gaggttcagc
     3781 agtggcagga gcacttagta tgttgttcaa cgcagctctt cgtgcacctc atggaggagt
     3841 atttgttata ccagtggtta cccatccatt tgcatatata ttagctatag ctgtaggtgc
     3901 tttggttgga atgatgcttt tagcattact taaaaaacca ttaaatcaag aagtatagca
     3961 attaaaggat attcaagaac ttaaaatatg atgttttata aaaattaata tattgctgaa
     4021 ttattttaaa ataaggattt tattaaaata aggattttat taaaataagg attttatgag
     4081 gctgttatat aacgatttgt aattgttatc ttgacagtct tttattttta tataagtaaa
     4141 ataagtgtgt tttgctttat ttgtatttaa attgttttta agttagaata taaataaaat
     4201 tatatattat tttaatattc actcgaacta gaa
//
LOCUS       DAEPLF010000260         4178 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEPLF010000260 DAEPLF010000000
VERSION     DAEPLF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.261"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(75..992)
                     /locus_tag="KR314_003306"
     CDS             complement(75..992)
                     /locus_tag="KR314_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBH0329663.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIETVKEFWE"
     gene            1254..1631
                     /locus_tag="KR314_003307"
     CDS             1254..1631
                     /locus_tag="KR314_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0329664.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            complement(1787..2236)
                     /locus_tag="KR314_003308"
     CDS             complement(1787..2236)
                     /locus_tag="KR314_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0329665.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(2414..2818)
                     /locus_tag="KR314_003309"
     CDS             complement(2414..2818)
                     /locus_tag="KR314_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBH0329666.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            complement(2931..3797)
                     /locus_tag="KR314_003310"
     CDS             complement(2931..3797)
                     /locus_tag="KR314_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBH0329667.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRSTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
BASE COUNT         1390 a          693 c          452 g         1643 t
ORIGIN      
        1 attattgtca atttattttt tcttaaaaat aaaataattg cataatttat ttcaaattat
       61 gcaatttata aaatttattc ccaaaactct tttactgttt cgattatctc actataaggc
      121 aataaagtcg cttcgattat tgatttattt gtcttttcta aataatactt aatcatataa
      181 tatccacaag catatcctgc acaataaggc aaccctactg gaaaatatcc ttgcatttta
      241 gctatatcat caccataaag ataagatgtt atattttgaa atccagtttc ttttaaagca
      301 ctttttatta ttggctttat ataagtattt aatgtttcga tatctgttct actgacccaa
      361 ggtcctaaca tttcctctcc aaacatccat gttgcaaaat tttctgcaag accttcattt
      421 atcatcattt cttctaatgt tatattatta ttccactcta taaactgaaa tctaatattg
      481 tgattacatt catgtgctat taatactggt aatctattga tagtatattc attaggaacc
      541 aatgatagaa atatatatcc aggaatccca ccatcacccc agtatccatc acttaatatt
      601 gtataaggac tatttggatt cgccaataat attgaatatt tatagtcctt aatgtttaaa
      661 tcataccctt cttttataaa agcatttata gagttttcta tggtttcttt gcaagtgccc
      721 caaattttat cagcagataa cacatttata ttcttttttt gcttttcatc aatatcctta
      781 ggcgataaaa cccctaacat ttcacttgct ataattacat catatccacc tttctgtcta
      841 gcaactattg gaacattcat acacttccac ttaaattcaa aaggcttcat caattcatat
      901 ctgtatatat cttctttttt gtttattggt gcatccatta tcttcttgta tattttatct
      961 gagcgaattt ctataatatt catatttacc atctccttta agacaaagta tatacaatta
     1021 cgtagcgtca atgtcaatat acatttttta taatgtcacc ttaatctttt tattatattt
     1081 tatcctgtaa accttccgta catataacat ttttttaaat taataaattt gtataatata
     1141 ataataaaaa attatatact tcaaggtttc cctatgatac aatttactaa atcataattt
     1201 agattatcag gtgcaactat aaattattct atatcaagga ggatatattg gtcatgctaa
     1261 ctccatattt aatatttaat ggtacttgtg aaaaagcatt taatttttat gctgaggctt
     1321 tcggaggagg aaaaactata tttgcgcgat tagacagcaa tccaaacaat cctattatgc
     1381 acgcaagtgt tactttcaca aaatacgaag gttgtataat gggtgcggat acagacaagc
     1441 ctgttgtaat ttctggcatg gcgatttgtg ttgttctacc atctcgagaa gcgatagaag
     1501 aaatatctgt aaaacttgcc gagggtggta cacttgtaca agaattttta ccacacccac
     1561 caccacatca aaatgatggc gctgctgaag tacttgatag gtatgggtat acttggtatt
     1621 taagtacata gtaaaaataa aaaaactaca tctaataatc ataatataga acagataaat
     1681 ttcaatttaa tagtgtagta aaaaagtcgt gtcctaagac tgtcacgact tttatttgta
     1741 taaaaattta agtaaatatt tttgcgaata agtgagaagt ataactttaa attaatttca
     1801 tatgtagtat tggaaatggg ttaccttgtc catctatttc agacctatca aacactttaa
     1861 atcctaagtg aatataaaat cctacagctt ttttattctg ctcatttaca tcaacatatt
     1921 ttacattgta tttatctata gcatatttta ttaatttttt accaatacca tttcctctat
     1981 atttatcttc tataaagagc atctcaattt tatctttatc tattcctata aagcccttta
     2041 ttatatcatt atcttttata caaactatat tttctacttc tttaagtcca atttcaacta
     2101 atggtatcaa agattcaata tctttttctg ttaaaaaatc atgcgttgct cttactgatg
     2161 attcccagac ttgcaacatt tcgtcatatt ctgttgaatt tatttgtttc atttcaagaa
     2221 ttattccaga tttcataaaa tttcctccct atatcacttt taattgtata acactttttt
     2281 atacaaaact tagaaaggac tacgaaggtt aagtatcata gtcctaatta agtttaaata
     2341 ttatctatta tatattttta acatttatta tatactataa tctagcaaat ttactacaat
     2401 aatccatatc agtttatact aaaggtgctt ctatttgagt agtaaaaagg aatttatgac
     2461 aatgaccatc attaacagta gtttctccac atattaagtg aatgtgttta tcagttccag
     2521 gtattctaat tgctggccct gttgtaacac atatcttatg gaaatggtca aggaaatcta
     2581 cattatcatt tattttatga acatgatttg tacctccatt tattggtata gcttcaccag
     2641 taacccctgc tgcacggtgg ttatgtctat cctcacattc ttctgctaac ttaacacttt
     2701 ctgaatattc atgaacatgt gtcatttcct cataatcatt atagcacttg ttgcacttat
     2761 tgtatttata atcatcatac acataatctc tttcacattt ataattttga taactcattt
     2821 ttatatctct ccctatatta tattaagaaa ttcttgtaaa gaatctctaa tgtataatat
     2881 gaattaaagt caaaaaagtt catattttgt aaaattatgt attaatttaa ttatttctcc
     2941 cagtatttag ggcgtattag tgaagctggt aacttcgtac tagaatctcc ttttgcagta
     3001 tttacttgca attgtgttaa aaatatacta ttttcaagat tagcacctct aatatcacaa
     3061 tctcttaagt ctgctcctat taaatttgcc ccactcaaat ctgtacttct taaatttgct
     3121 gctattagaa atctacctct taaatctgct cctattagat ttatttttct aaggtctgct
     3181 cctattaaat ctatattttt acttaatttt ttcatatttt taagagtaga agtcttgata
     3241 aatgatttaa cctttcccat cacagactca ctcgcttgtg ataataataa attaaccttt
     3301 gttctatgtg atatgatatc caaattaatt atctgttctg ggctcatatt agttatcttt
     3361 tcagtttctt caattatcaa atcaattttt gatttgattg tatctgatat atttaataca
     3421 gatgcttctt taagatacca taacatttca tgtaattgtc tcataattaa aaacacttca
     3481 gacatttgat ttgttaattc tggtgattgc atccagtcta tccccttata tgttacttgt
     3541 gctacttttt gacctgaacc aaaacaatca tatgcagtac accctttaaa acctcgcttc
     3601 ataagactat tatgtacaga acatttaaaa tctggttgta aattaataca gggttttcct
     3661 gattccttat cgataggaaa tccatctgaa gcagaaaaat aaagagctac acaacaaagt
     3721 ccaaaacact tcttacaatc aatctttagc tcctcaaata acttattatc atatatattt
     3781 ttatgtatat ctatcatatt ttttctcctc ttaagcttta tttttaatat tatattatct
     3841 ataactaaat gataatcaag tcccttattt aacagttctt attcttaatt ataatttaag
     3901 gtattattta ttccaatagc atacataagt ttctttatac ttgtaacaat acctttatac
     3961 taagacttat tttaaatgta gcactgtaat aataaattgt ttatatactc ataatgtatt
     4021 tgataaattg taaatcttta tagaagttga aatttttaat gattttagaa tcaagatata
     4081 ataataaata caatttataa aatcagaaat atataaatta actatacaaa cctaaataac
     4141 atagcattta aattcacaat ttcagtataa atagtaca
//
LOCUS       DAEPLF010000261         4080 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEPLF010000261 DAEPLF010000000
VERSION     DAEPLF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.262"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(109..1128)
                     /locus_tag="KR314_003311"
     CDS             complement(109..1128)
                     /locus_tag="KR314_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBH0329668.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLHGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQAITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(1304..1843)
                     /locus_tag="KR314_003312"
     CDS             complement(1304..1843)
                     /locus_tag="KR314_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBH0329669.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPILVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(1944..2624)
                     /locus_tag="KR314_003313"
     CDS             complement(1944..2624)
                     /locus_tag="KR314_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBH0329670.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(2653..3942)
                     /locus_tag="KR314_003314"
     CDS             complement(2653..3942)
                     /locus_tag="KR314_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBH0329671.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         1365 a          732 c          373 g         1610 t
ORIGIN      
        1 cttataattt gacattttaa cacttggcta agtatacacc ttaccttaac ttaaaattaa
       61 atttatttaa gaatatatat tataaattct tatattttct aatataagct atatactatc
      121 agctggataa acaatactac tttgtcttct tatctcatcc ataatttcca ttactattat
      181 tgaattttca tgagaattta tatttgattc tattttattg ttatttataa gacttataaa
      241 ctctacaagt tcataataca ttccttctat ttctctaggc ttttcctctg ctttttcttt
      301 tggaatagat atatcttctt ctcttccatc tctgtattta ataatagctt tttcaaatag
      361 attaagcttt tcaattatta tacttccctc ttctccttgt atttcagatg gtatataaga
      421 atcagctact ttagaatact gaattattgc atccatatca tcatattgaa atacagctga
      481 accttctcca tccactccag tttgaagtat atgagaaact gcctttacag tttttggagc
      541 accaaataaa ttcaccatag gatgtataca atacactcct atatccatta aagctccatt
      601 tgacaactct tttttaaagg cattttctat aattccattt ttatatttat catatcttga
      661 agaatactga caatagcttc caaaaaatct tcttattttc cctattttat acaagttttc
      721 tttaactgct ttaaagtttg gaacacatgt aattctcata gcttccataa gcaatacatt
      781 attatctttg gctgttttta tcattgattt gacttctttt aaattagatg ctaacgactt
      841 ttcacaaagc acatgttttt tgtgttttaa acaagtaatg gcttgctttg aatgtaatgc
      901 atttggagaa gctatatata cagcatctat atccttcgat ttagccattt gttctaaatc
      961 atcaaaaaaa atacttgctc catgtagagt tccaaattct ctagcttttt ctaaatttct
     1021 agaatataca gcaaccaatt caaaatcttt aaccctagat gcagctctta gaaatatatt
     1081 tgctatattg cttgtcccaa caataccaaa tcttatttta cccatcataa atcactcccc
     1141 attttctaca ctttatttta attttaataa aaaactttac aacttataga tatattataa
     1201 aataatttta tcaaataaaa aagaaagcta attttaaatg atttcaccat tttaacatag
     1261 ctttctttta actacaatct aaattcaact tatattttta aagttatctt tctactatat
     1321 ctttatttcc cctcaaaaat tttatagtaa gaatttcatc cacaaacaat gttactaaaa
     1381 gtatagttac aagaattggc caataaggta ttataagact atataaacta tcaactatat
     1441 acacttgaaa atatctaact ccaataaatg tcaatatagt ccaatataca gaaaacttta
     1501 gacatactat tcctctacta ttgtacttat aatctgaata atcccaatag accttattaa
     1561 aatatacttt cataagtatt cctgtaatat attctactac tgtcggcact actagacata
     1621 ataaaagcaa tgcaaatata ttattggggt caataaatct tgagacctct attataatcg
     1681 acattgcaat tgcatacatt ggtttaaatg gaccatttaa aaatccatct tcttgaaaat
     1741 gcccttgttt ataataacta tataagtttt caattatcca acctataaaa ccatacaaga
     1801 aaaaattaaa taaaaaatat aatagagaac ctacattatc catgaatatt cctcctagct
     1861 gtttatactt attttttctc atattcatgg atttattcta aatcaaagaa attatattta
     1921 atttatttaa ttaatacttt atattataga ttgtgacatt ctttagagtt caaacttttt
     1981 aggtattctc taacttccag taaattttca agtatcttat gtgattgtga tttaatttgc
     2041 tcatctggct cttttaaatc tggtacattt atacatctta tcccaccatt gtatgcagct
     2101 tcaactccca taggcgaatc ctctatgaca atacaatttt ggggattaac atttatcttt
     2161 ttagctgctt ttaagaatat ctcagggttt ggtttagagt ttacaacatc atctccacaa
     2221 actatagcat caaagtaatc ttttaaattt gccttagcta atctctttac tgctctttct
     2281 ctctttgttg atgttgcaac agccattttg tatccatttt cctttaaaaa agagattagt
     2341 tcattgactc ctaatttgat aggagctccc ttcctctcca taaattcaat catattttta
     2401 gttttttcgt catataaatc tattattggt acagaactat cgtatatatc tgtaagacct
     2461 tctattattc ctttacgatt tcttcccata actgatgtat aaatttcctt agtcatagta
     2521 tatccatact tttcaaacgt ctccatccaa aattcaagag aaattctctc tgagtcaaac
     2581 aaaactccat ccatgtcaaa aataattcct tctacttttt gcatacaatt cctccaatat
     2641 tttttgatat aattatatta cactttttat gattaatttt acaaccctca atttatccaa
     2701 ttctaatatt gtaaaattgt aattttcaaa agttacgctt tggtttactt taatatttga
     2761 accaagcata taggatatcc acccacctat actgtcaaat ccatcatgct ctatatcaag
     2821 tccaaaatac ttattaatat agttgataga ttcagttcca tcaactaagt aagaacctgc
     2881 tcctattttc ttaatatgag tctcttcttt gtcaaattca tcttgaattt ctccaactat
     2941 ctcttctagt atatcttcta ttgttaccac accagatgta ccaccatatt catccacaac
     3001 tatagcaagt tgaagttttt cctttcttat tctctcaaga gctttatcta tagttaaatt
     3061 ttcagatata tatataatat ctctcaatat ttcttctagt tcaattttat tttcatttat
     3121 cttttgattg tataaatctc ttatatgtac aaaacctaat atatcatctt tattttttct
     3181 gcatactgga tatcttgtaa caccttcctc ttttaaaata gctaatatct tttcttcaga
     3241 atcactttca tatatacata ccatatcagt tctaggaacc attatctctc taattttctt
     3301 ttcttcaaat tcaaaaatat tatcaactaa tctttgctct gattcatcta taagtccact
     3361 tttataactt tcttctacta atagtcttat ttcgtcacct gtatggggtt catcaacctc
     3421 atcagcttgt gaatatccaa atggtttaag aaataaatca gtacttaagt tgaaaaaata
     3481 tattattgga tatgttaact tataaaatcc tactaataaa aaagatgtac tcaacattat
     3541 tttttctgta ttataaagtg ctaaagcttt aggaaccaac tctcctatta caacctccat
     3601 cattgttata attaaaaaag aaatggaaat tgatatagta tatataatac tttcactaag
     3661 attaaataca cttactattg gtaaaattaa ctctttaact gtagattctc ccatccaacc
     3721 taatgcaagt gaacaaagtg ttataccaag ttgacatgca gaaagatagg aatttaagtt
     3781 gtcttttatt attttacatc tttttgcatt attattacct tcagttatca tggtatcaat
     3841 cctagaacta cgaagtttta ccatagcaaa ttcacaagct acaaaaaaag catttatcaa
     3901 aacaagcatt actataagtg ttacttttaa taactcccgc ataaagtatt ggttcttata
     3961 atgtaagaaa tctataagaa ctcaaagttc cccccttata tttataaatt taatttataa
     4021 tatatattct atcatattta ttaatatatc ctactttatt acaaataatt tttataaact
//
LOCUS       DAEPLF010000262         4077 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEPLF010000262 DAEPLF010000000
VERSION     DAEPLF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.263"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            175..366
                     /locus_tag="KR314_003315"
     CDS             175..366
                     /locus_tag="KR314_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBH0329672.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            657..3158
                     /locus_tag="KR314_003316"
     CDS             657..3158
                     /locus_tag="KR314_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBH0329673.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNVLAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            3318..3986
                     /locus_tag="KR314_003317"
     CDS             3318..3986
                     /locus_tag="KR314_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0329674.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
BASE COUNT         1687 a          412 c          760 g         1218 t
ORIGIN      
        1 aaacaacaga taccaccgta catgtagtat taataatata taaaattaat atgtaatact
       61 cacaatagga tataaatggt atatatggta ataatttata ttaaaaaaac ttttataaag
      121 tttatatttg ggtataaaag tattaattag acattaatta ggaggaactt aaatatgata
      181 agagtatgtc caaactgttc aaatgtggat gtaaataagc taaaaacttt ggttggagaa
      241 gaaaatgtaa agactggttg tataggtcag tgtagagcat ttaaaaaaga agctgttgga
      301 ataatagaca atgagcttga aataaaggaa acagaagcag agttttttga agcatgtaaa
      361 gaataaattt taaataaaat ataaattata ataaatagag catctaaatt gtataagcat
      421 taaacaattt agatgctcta tttttatata tacatttaat tatgagtata cttttctatt
      481 aaatacaaat aaatataact aatacattta tttattaaaa aaatattgat ttaatatacc
      541 tatagggggt ataataaaaa tatagatttc taaataagta atttatcaat attacatatc
      601 aaaataaaac gttacaaatt agctgaaaat atattgaaag aaaggaagat tttattatga
      661 gttcaaataa agtggtagaa agttataaaa ttacaggtat gacatgtgca gcttgtgcaa
      721 aggcagtaga aagagtaact aagaagatgg atggagtata tgaccaaagt gtaaatatag
      781 ctactgaaaa acttaaaata gaatatgata attctaaggt taattttgat gatataaagc
      841 aggttgtaga aaaagcagga tatggaatta taaaggaaga aagtaataaa aaaattgata
      901 tgaaaattga tggaatgaca tgtgcagctt gtgcaaaagc tgtagagaga gtggtaaaaa
      961 aattggatgg agtagaaagt attagtgtga atattgcaac agataaagct aatatagact
     1021 atgacccatc aaaagtaaaa ttatctcaaa taaaagcagc tattgaaaaa gctggatata
     1081 aaccaataga agaggtaaga aataaggttg atgtggatga agataaattg agaaaagaaa
     1141 gagaaatgaa gtctttattt gttaaattta tagttgcaat agtatttgca gtacctctgt
     1201 tttatatagc aatgggacct atgataataa agcctatagg gccatggcct ttgccagaaa
     1261 ttataaatcc tatgacaaat acttttaatt atgcattaat tcagttaatt ttagtaatac
     1321 cagtaatgat agctggttat aaattttata taaatgggtt taaatcactt ttttctttaa
     1381 gccctaatat ggattcttta gttgcaatag gaacattagc cgcattttta tatagtctat
     1441 atacgacact acaaatagcc aatggacaaa ttcaaggtat gcaccatcat cagttgtatt
     1501 atgaaagtgc aggaataata atagcactta tcttacttgg taaatattta gaatcaaaat
     1561 caaaaggaaa aacatcagaa gcaattaaaa agcttatggg gctacaacct aaaactgcaa
     1621 tagtgttagt tgatggtaaa gaagttgaaa ctcctataga agaagtagag ataggagata
     1681 tactattagt aaaaccagga actaaaatac ccgtggatgg agttgtaata gaaggatata
     1741 catcagttga tgaatcaatg cttacaggtg aaagtatacc agtagaaaaa aatgttggaa
     1801 gtaaggtgac aggagctagt attaataaaa atggtgttat taagtttaag gctgaaaaaa
     1861 ttggtggaga tactgctctt gcccaaataa taaagttagt tgaagatgca caaggtacaa
     1921 aagcacctat tgctaaactt gccgatactg tatctggata ttttgtacca atagtaattg
     1981 caatagctgt agttgcatca ttattgtggt ttttaatagg aggaaaagat atagtatttg
     2041 tacttactat atttatatca gtacttgtaa ttgcttgtcc ttgtgcatta ggattagcaa
     2101 caccaactgc tataatggtt gggactggaa agggcgcaga aaatggaatc ttgataaaag
     2161 gtggagaggc actagaatct gcacataaag taaatacagt gatatttgat aagacaggta
     2221 ctataacaga aggaaagcca aaagtaacag atatagtatt aaataataat gtaaaagaag
     2281 aatatcttat caagattgct tcaagtgcag aaaaaggttc agagcatcct cttggagaag
     2341 caatagttaa gtatggagaa gagaagaata taaaatttga aaaagttgat aattttaaag
     2401 ccataccagg tgctggtata caagttacta ttaatgatga aagtatatta cttggaaata
     2461 gaaagcttat gaatgacaat aatataaagc ttggggattt agaagaaaaa tcaaatgtat
     2521 tggcaagcca aggtaaaaca cctatgtata tagctgtaga tggaaattta tcaggtataa
     2581 tagcagttgc agatgtagtc aaagaaagta gtaaaaaagc aatagaaata cttcatgata
     2641 tgggaataaa agttgcaatg gtaactggag ataatgcgaa aactgcaaat gctatagcaa
     2701 atcaagtagg tatagatatg gttttggcag aggttttacc agaagataag tcaaaagaag
     2761 ttgaaaaact acaaaatcaa ggaaagtttg ttgcaatggt aggggatgga ataaatgatg
     2821 ccccagctct tgcaaaagca gatataggga tagcaatagg aagtggaacg gatgtagcca
     2881 ttgaatctgc ggatatagta cttatgaaga gtgatttaat tgatgttcca acagcaatta
     2941 aacttagtca tgaaactata aaaaatataa aacaaaacct attttgggca tttggatata
     3001 atacgattgg tatacctgta gcagcaggaa tattatatgt atttggagga cctttattaa
     3061 acccaatgat tgcagcagca gcaatgagct taagttcagt ttcagtcgta tcaaatgcac
     3121 ttagacttaa aaacttcaag gcatataaga gagattagat taataaatta aataaaatgt
     3181 atccaaaaga gctatcttta aattaaaagg attaatttac aaattaagat agctcttttt
     3241 tcatgtaaaa ttcacaacaa taagataaaa tataagtaat atacgaaatt ttaaataaaa
     3301 tagaaaggat tgatagaatg aattcgtcaa tacttgtaat tgaagatgat tcaaatatac
     3361 aagagttaat atcagaattt ttaagtgcag aaggttatca agttgataca gcaaatgatg
     3421 ggttggaagg tatacaaaag tttaagcaag gaagttatga tttagtaata ttagatataa
     3481 tgatgccaaa cttagatggt tatggagttt gtaaaatgat aagaaagtca tcaagcgttc
     3541 caattatatt tttaactgct ttaaatgatg aaggagacca acttaaaggg tttgacttag
     3601 aatgtgatga ttatataaca aaaccatttt catttaatct tcttataaaa agagtagaag
     3661 caattttaag gcgaagtaac aagactataa atgacaaatt tatagttttt gaaaaattaa
     3721 aattagattt aaacacatat attgcagaga tagatggaga acctatagaa cttacactaa
     3781 aagagtttaa tatattaaaa gctttgatag aaaagtatcc acaagttata actagggaag
     3841 gtcttttgga tagtatatgg ggttatgact attatggaga tactaggatt gttgatgcac
     3901 atataaaaaa tataagaaaa aaaatatcgt taccatatat taaaactgta aaaggaatag
     3961 gatatacact tgagaaggat atttgataag tgggaaaagt taagtataaa gtataagtta
     4021 ttttcaataa cgacatcatt attaatagcg ttagctttaa taatatatct aatttta
//
LOCUS       DAEPLF010000263         4066 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEPLF010000263 DAEPLF010000000
VERSION     DAEPLF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.264"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..1880
                     /locus_tag="KR314_003318"
     CDS             <1..1880
                     /locus_tag="KR314_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBH0329675.1"
                     /translation="RDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALF
                     IIGYVFSYDLKRAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNR
                     NRMKNVGDKELISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLK
                     MVIFDNRKDDFYISIRGIDIMNILFSLERISLIMERYKKDSKEFEDEDIKNILEESSI
                     NSKSDVLVVATKEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLEN
                     LYEFLVDYKKVKSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAV
                     AGFIMDYFHLRDGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKD
                     STLRSLIVLLAGYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAI
                     IALIVNKFILPYDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSL
                     IEDRLMLINAIYKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYI
                     SNYNVDKYDEGRSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEA
                     A"
     gene            2095..2952
                     /locus_tag="KR314_003319"
     CDS             2095..2952
                     /locus_tag="KR314_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0329676.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            3049..3918
                     /locus_tag="KR314_003320"
     CDS             3049..3918
                     /locus_tag="KR314_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0329677.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT         1705 a          361 c          683 g         1317 t
ORIGIN      
        1 aaagagattt aactatttct ccaattaaaa atttactgaa gtatttagca ataaatataa
       61 tcttaggaat actatcattt tttgcagtac aaaatatgta tttaggagtt ttattaaatt
      121 ttatagcatt atttataata gggtatgtat ttagttatga cttaaagaga gcagtatatg
      181 taccttttgg tctaatgtat atatttatgg taagtatacc tgtaggaaaa agtgaatttc
      241 ctatgagatt gtcggcttta gctgttggag cagttataat aatgattgct caatttgtta
      301 tgaatagaaa taggatgaaa aatgtcggag ataaagaact gatatctata tgtgatgaat
      361 tattagaaaa aattagtctt ttaaaaaata ctattaatga tgatagttct ataattaaat
      421 ctagtatgag aaaaatagat agctgtaatt atagaataaa ttctatatct aaaaatttga
      481 aaatggttat ttttgataat agaaaagatg acttttatat cagtattaga ggtatagaca
      541 taatgaatat tttattttca ttagaaagaa taagtttaat catggaaaga tacaaaaagg
      601 atagtaaaga atttgaagat gaagatataa aaaatatttt agaagaaagt agcataaata
      661 gtaaatcaga tgttctagtt gtagcaacga aagaaataaa ctatattaag atgtgtttag
      721 aaaataaaga taccataact gataaagaga tattaggatt tagagattat gttattgctc
      781 aggatactaa aaatataaat ttaaaagaaa tttatagtgt tttagaaaat ttatatgagt
      841 ttttagtaga ttataaaaaa gtaaaatcaa gagacgaaaa gaaagctgaa agaaaatcaa
      901 agatacctca tgaatttaaa cgactatcaa tatataagaa aaactttaat ttaaattcta
      961 taagattttc ttatgcagta aagattgctt tagctacagc tgtagcgggt tttattatgg
     1021 attatttcca tttaagagat ggaagatgga taatgttaac tgtcttttca ttaacacaac
     1081 cttacgcaga aaattgtata caaaggtcta gaaaaagaat agaaggtaca tttataggag
     1141 ctgtaatatt tattgtatta ttttcaataa taaaagatag tacattaagg tcgttaatag
     1201 ttcttttagc agggtatata aactcttatg tagttgatta tagaaaatta atggtatgtg
     1261 taactgtttc agctcttggt tcagcagtag ttatgggaga cccaaatgtt ttaaccataa
     1321 gcaggatttc atatgtagca ttaggagcaa ttattgcatt aatagtaaat aagtttatat
     1381 taccatatga tgctaaaaca ggctatcaac atgttataga aatgtataaa ggaatagtta
     1441 aaaatataat tgatgaagtc aataagtcta tagaaaatgt agctgatgtt tactatataa
     1501 aaaatttatt attgatacca tcacttattg aagatagact tatgttgata aatgctatat
     1561 ataaagataa acatcaagag gattttttag aaaatcagaa attactaata agtaatatgt
     1621 ataatctata tataaatgtt aataaaaata aaataaaaga tgaagatgtt gaaaaaatat
     1681 tgagagatac taattatatt tcaaattaca atgtagataa gtatgatgaa ggaagaagtg
     1741 taattttaga gagtattgta aatactaaaa gtctaggtga caagataata tgtctaaact
     1801 tactgcaaac tctaaatggt gtcaaagaaa tgtatagaat aagtaatata actaaagttt
     1861 caataaaaga agcagcttag atattaaatt atagaatagc catatattta aaataaagta
     1921 tatggctatt tgttttataa aatactaaaa gaaaataata tataaaaaac aaaaaacata
     1981 tatttatgaa ttttatatga aaatagttaa aatattgatt tttttcattg gttgaaaaaa
     2041 caatttactt aggataaact ataatagtat tcaaatatta tgggggttag atttatgagt
     2101 aaagatgtaa aggtaaagcg taataagaaa tatgatacat ttagtataac tatggaactt
     2161 gagagacctt tactttatga tgaggttgag aacaaagatg ataaaaataa taatagaaaa
     2221 tttaacataa agaataagat atttaaggga ataggtatac tatttgtatt gattttgctt
     2281 ggctttttca tatggataaa taaaacatat gaacctcaaa agctggcaaa agaagctcta
     2341 gtcagcaata gtaaagttga agtaactgta aatgaaaaca tttcatttac accaaaagga
     2401 agaaaagttt ctaaaggttt aattttgtat ccaggagcta aagtagaaat agaatcttat
     2461 gctccacttg caagaaaaat agcagaaagt ggatatgagg ttgtaatcgt aaagatgcca
     2521 cttaatctag ctatacttgg aactaataag gcacagaaag ttatggattc ttacaacaac
     2581 atagaacatt gggtaatagg aggtcattca ttaggaggag ttgcagcatc aaattttgca
     2641 cgtgataata aattaattga aggcgttgta tttttagctt cttatccaat gggtgatgag
     2701 ttgaaagaat taggaaaaaa agttatatct atatggggga gcaaagatgg agtagtcaat
     2761 tttaaaagtc ttgtagaatc aaaacaaaaa cttcctgatg atacaactta tgttgagatt
     2821 gaaggaggaa atcatgcaca atttggagac tatggaaaac aaaagggaga taatgatgct
     2881 attataagtc aagaaaaaca acttaatatt actgccaata gtatcattaa atttttaaag
     2941 aatatatctt aaactaaaat ttaaaaaata taattttata agttattatt taaagtaaat
     3001 attaataata taatagaatg aatattttat tacaaggggg attttaatat ggaatacgat
     3061 atgccacttt acagaccacc aagtgaggct tatagtctta taatacaagt cacactagga
     3121 tgttctcata acaaatgtac gttttgtagt atgtacaaat ctaaaaaatt taaaataaag
     3181 ccactagaag taataaaaaa tgaaattgat atatttagaa gatattataa gcatgtagaa
     3241 agaatattct tagcagatgg agatgcattg ataataccaa tggagaaatt aagagatatt
     3301 attttatata taaaagaagt ttttccagag tgtgaaagaa ttactatgta cggctctcct
     3361 aaatctatag agaaaaaatc tgatgatgaa cttaaagaat taagaaaact tggtgttaaa
     3421 atgatttatc tagggcttga aagtggaagc aatgaagtat tagaagatat taagaaaggt
     3481 tttagtagtg aacaattaat aaaagttggt agaaaagtta aaaaaagtgg gattaaatta
     3541 tcagccactg taatagcagg tcttggagga actaaaaaaa cacatcagca tgcagttgat
     3601 acaggaaaaa tgttaggagc aatttcacca gattatgtgg gtgtacttag tttaatggta
     3661 gaacctaata ctgaacttta cgatttactt aaggcaggag agtttacaat actagaggat
     3721 aaggctgtgt tacaagaaat taaagagatg ataaagaata tagatgctaa tgaaaaaatt
     3781 gtatttagaa gtaatcatgc atcaaattat gctaatttaa aaggtgtatt acctgatgat
     3841 aaacaacgtc taattgatga aattgattac tatctaagta accaaaagct aaaaagagaa
     3901 gaatatagaa gactatagtt tttgatatat caatctatat tgataggtaa atactaagat
     3961 tatttagata caaaaaatca tgctacatta atagttgctt actactgatg tagcatgatt
     4021 tttttattaa aaaacaaaat ggtatattca tatataatat atcaat
//
LOCUS       DAEPLF010000264         4022 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEPLF010000264 DAEPLF010000000
VERSION     DAEPLF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.265"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            500..1771
                     /gene="serS"
                     /locus_tag="KR314_003321"
     CDS             500..1771
                     /gene="serS"
                     /locus_tag="KR314_003321"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBH0329678.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKHKLNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            2139..3644
                     /locus_tag="KR314_003322"
     CDS             2139..3644
                     /locus_tag="KR314_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH0329679.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            3758..>4022
                     /locus_tag="KR314_003323"
     CDS             3758..>4022
                     /locus_tag="KR314_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBH0329680.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYF"
BASE COUNT         1687 a          396 c          654 g         1285 t
ORIGIN      
        1 attaccttat aacatttcat ataaaatata ttgtgaatct gagaaaatta ctatttttct
       61 ttgagtattt tttataagca gttctaaaag attcaatttc atacctatta cttgaagcat
      121 tcattaaaat aattctaatt taaaaaataa ttctatccaa ataatagata tataggacta
      181 tgttataata taaaaaaatt tatataaaag ttaataacta tacaagattg ataaagaaaa
      241 agtcaaaata gataattgat tagcaaaata gatataatta taatgaagtt aaatctagca
      301 atattctcct aaaattaaat ttcatttatt taattaaata taatttgaca atggacaaaa
      361 aagtgagtaa aatataaata aaataaaaat aacaggttcg gataaagaaa aaggtttctt
      421 aacctcaagc ataaagtata ttatctaagt tatttgagat taagagataa atccaatctt
      481 aggtactgag gaggaaaaaa tgcttgatat aaaaagaata agagaaaatt tagattgtat
      541 aaaaaaagca atggaaagaa ggggcgaaaa agactttaac cttgatgaag tagttaagtt
      601 agatgatgaa agaagaaaga ttcttcaaga agttgaagtt atgaaacata aattaaatac
      661 agcatctaaa aatataccaa atcttataaa agaaggtaaa gatgtagaaa atgaaaagat
      721 taaactaaaa gagttatctg ataaaattaa ggtaatagac caaaatctta aagaagtaga
      781 agataagatg gaatacctat taatgagaat accaaatgta ccacatccag aagtaccaca
      841 aggtgaaact gatgaagata atgttgaggt aagaacttgg ggaaagacta ctacctttga
      901 ttttgaatct aaggctcatt gggaaattgg aacagaatta ggtatacttg attttgaaac
      961 agcgagtaaa ataactggtt caagattcac tctttacaaa ggattgggtg ctagattaga
     1021 gagagcatta ttaaattttt acttagacac gaatacaaaa gtaaatggat atacagaagt
     1081 cattccacca tttatggcaa atagaaatag ttttttaggt acaggacagt tacctaagtt
     1141 tgaagaagat atgtttaaaa tagaaggtct ggactatttt atgattccaa catcagaagt
     1201 tcctttaact aatatacatg ctaatgaaat acttaaattt gaacaattac caataaatta
     1261 tacagcttat actccatgtt ttaggtcaga agctggttct gctggaagag atacaagagg
     1321 tttagtaaga cagcatcagt ttaacaaagt agaaatggtt aaaatagttg caccagaaga
     1381 atcttacaat gagttagaaa agcttactaa caatgcggaa actatgctac aattattgaa
     1441 tctgccttat agagtagtaa agatatgtac aggtgattta ggatttacgg cttcttttaa
     1501 atatgatgta gaagtttgga tgccaagtta caataggtat gttgagattt catcttgttc
     1561 aaactgcgaa gatttccaag ctagaagagc aggaataaga tttaaaagag ataaagattc
     1621 taaagctgaa tatgtacaca cattaaatgg ctctgggtta gctattggaa gaagtgttgc
     1681 agctatatta gaaaattatc aacaagaaga tggttctgtg gttgttcctg aagttttaag
     1741 accatatatg ggagtttctg ttattaaata agagttaaat tatgtatttt taagcaatac
     1801 aataatatag aagtaataaa aaataattaa tataaaagtg atacaataat atagaagtaa
     1861 taaaaaaata gttaatataa aagtgataca ataatataga agtaataaaa aatagttaat
     1921 ataaaagtga tacaataata tagaagtaat aaaaaaatag ttaatataaa agtgatacaa
     1981 taataataga agtaataaaa aaaaataatt aatataaaaa tgtgtacaaa ctaatgaata
     2041 ttgatatagt aagctatcat atgaaagagt atgtacacat tttttttact aatttttcac
     2101 aatatgaaca taaataattt acaggaaagg aatcttttat gaaaattcaa aatactgagt
     2161 ggggatatat tgaatggaaa catacttatg atgaaaataa tccaaaacaa gctatgaata
     2221 tatacattgc agtaactatg cctggtaaga aacattttaa tcatgttcat tatggtcaag
     2281 agcagatgat atatatttta gagggagagg gtttgtatat tataaatgga gtttggaagc
     2341 cattttatca aggtatgatt ttttatatag aatctggtag cacacatgaa actataaata
     2401 caggcgatag agaaataaaa gaacttattg tttcaaataa tgtagatgat gtaggagaga
     2461 gtgaagttat agatattaat cctaataatt atttaaagaa gaccttgata aattatagtg
     2521 aaagtacatt aaatttgtat gcagcagtag aatcaattcg tggtcaattt atagacccgt
     2581 ttaaaattcc attaattata tatgatgatt catggaatat agttttaaaa aacccttatt
     2641 ttccactttt ttgttttgaa aaatgtaatc ctatgaaatt tcctcaaaat tgtgattgta
     2701 tgaatcaaaa aagtagcaat caatttgtat gtgaatatgg gataactata tataatatac
     2761 caatactgta caaaagcaat agcataggtg ttataagagg agggtatgta ctattatctg
     2821 atttaaattt agatacagag cataataatt tatatgatat tccagaggga gctgctagaa
     2881 gtataaaacg cctcttgaaa caaatatcta aaaatataat aaatttttgc tcttttaatg
     2941 atataagaaa ggatttacaa gaaaaagaga agactatagc aagaacatat cattatggag
     3001 agcaacttga aatgaattta aaagtagctc aagatatggt tactaattta agaataaatc
     3061 atcacttttt atttaataca ttaaattcaa tggcaagcat agcactagat gatggtagtt
     3121 atgatttgta tagtgcaata atagatttat caagaatgtt tagatataca atgaggtctg
     3181 atttaagatt tgtagaatta gaatctgaaa tattatacat taaaaattat ttgaatttac
     3241 agaaattacg ttatggagat gcacttaaag taaaatatct tattccagag aagttatata
     3301 acctttctgt gccttttaac tttattcaac ctatcgtaga gaatgcattt acacatggat
     3361 ttagagatat tgacacagaa aagcgaattg aaataattgc aagattagat tctcagtatg
     3421 ctattataga gattcataat aatggaacta tcttagatgg aaataatata gacaaaataa
     3481 aggcaggtat tcgtagtaat aatggtcatg gtttgtcttt aatatataca aaattcacat
     3541 ctgcttatgg aaataatttt gatatggata taaagtcgag tgataatgaa ggaacttaca
     3601 taatgattaa aattcctatt gaaaattata aggagtatgt atagaacttg tgaaaaatta
     3661 ataagtttta taaatgttaa gaaatatatt agatgttttt atgttgtttt atatatctta
     3721 cttttttata ttattaagct aaaagggagg tattctcatg agaagagcac taataataga
     3781 tgatgaagaa gctgttctaa aaataataac acattttata gaaatgaaag atatgccaat
     3841 aaaaattgtt ggtaaagcaa catctggtga tgaagcagta gataaaatca ttggtttaga
     3901 gccagacata gtttttatag atattcaaat gccaatatac aatgggttag aagtaataga
     3961 aaaaacatct catttatcta aaaaaataaa ctttgtagta ataactgcat ttaactattt
     4021 tg
//
LOCUS       DAEPLF010000265         3969 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEPLF010000265 DAEPLF010000000
VERSION     DAEPLF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.266"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            76..996
                     /locus_tag="KR314_003324"
     CDS             76..996
                     /locus_tag="KR314_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBH0329681.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            1111..2268
                     /locus_tag="KR314_003325"
     CDS             1111..2268
                     /locus_tag="KR314_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBH0329682.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(2353..3852)
                     /locus_tag="KR314_003326"
     CDS             complement(2353..3852)
                     /locus_tag="KR314_003326"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBH0329683.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         1572 a          555 c          607 g         1235 t
ORIGIN      
        1 agtaataagt aagtaattgt ataatcaaac tgcaatatat taataatatt agtgaaaatg
       61 aaaggattat aaaatatgta taaatcagaa aatttttcaa aaaaatctgt attaataaat
      121 tcatttttaa gtaaattttt aaaaataatt ggatggggtt tggtaagctt atttacatta
      181 tcagccttag tactttcaac tatttccatt ataattggat gggctgtaat acttcctatt
      241 caagtatgtg cagtattttt acttatagga ggagtttgta tttttaaagg taagagatta
      301 ggagaccaaa tttctcgtta taaaaaatat tgtacaatta taaacaatag aaatattatt
      361 ccagtagaac ttattgcaga aaacacatct aaatcattaa attttataat aaaagatgta
      421 caaaagatga tagacaaaaa ctactttata aatacttata ttgataaaag aaataatcag
      481 attgtgttaa caaatgaaga ttttattcca ccaagatatg aagatgtagc atatgaagtc
      541 aaagaagaag aaaagactga tgaatccaat gaagttgata caattataaa taaaggaatg
      601 aattatctaa cacaaataaa agaagctaat aaaaatatta aatcagaatc aatgggcgat
      661 aaaattgtac aagtagaaga tgttacaagt aaaatatttg atgttgtaaa acatgaccca
      721 tcaaaactta ctcaaataca aaaatttatg gattattatc ttccaaccac tttaaaacta
      781 ttaaattcat atcatacatt ggaagaacaa ggaattgata gagaaaatat aacaacaact
      841 atggaaagta ttgaaaatac aatgaatacg attgtagtag cttttgaaaa tcaattggac
      901 tatctgtttg aagatgaagc cattgatatt tctacagata ttactgtttt agagaatatg
      961 ttagtccaag aaggattaac gagtggtaaa ttttaacttc aatagaaaaa atattctgtt
     1021 atttttaaac aattaattag tcataaagta taaaatgtgt attatataag ttaatcagta
     1081 ctttatgaag gaagaggaga gaaagataca atgaataatt tagatgatat accagttatg
     1141 ccaactttga cacttgaccc atttggagaa gaatcaaaca ctaatgatat agataattct
     1201 gacttattaa tgaaaaagga tgaaaaagac ccagaagaag aaaaactgtc tgaatctgaa
     1261 cgcaagatgg taaaggaatt tgcggaaaaa atagatatta caaatacaaa tatgattttg
     1321 caatatggag ctggtgctca aaaaaaggta gcaagttttt ctgaaacagc tttaaagagt
     1381 gtaaaaacaa gagatttagg agaagttgga gatatgctta caaatctagt tactgattta
     1441 aaagcatttt cagcagatga aaaagaacaa agtggttttt taggaatttt taaaaaggca
     1501 aataataaaa tttcaaattt aaaaactaag tatgatagtg cagaggtaaa tgtggataaa
     1561 gtaagtaaag agcttcaaaa gcatcaggtt aaaatattga aagatatagc aatgcttgat
     1621 aagatgtatg aattgaactt agcttactca aaagagctta ctatgtatat tatagcagga
     1681 aaacaaaaat taaaggatat gaaagaatat gaaatgccaa agttgagaga aaaagctcgt
     1741 ttatctggtt caacagaaga tgctcaatct gtaaatgata tggtttcact ttgtgacaga
     1801 tttgaaaaga aaattcatga cttagaatta actagaatgg tatcgcttca aatggctcct
     1861 cagataagat tggttcaaaa tagtaataat ctgatggcag aaaagattca atcaactata
     1921 gtaaatacca ttccattatg gaaaaatcaa atagtgcttg ctcttggaat ttctcactta
     1981 aatcaagcta tgaaagcaca gcgtgaagtt agtaatatga caaatgagct tttattgaag
     2041 aatgcagaaa ctttgaagat gggaacaata gaaacagcaa aagaatcaga aagaggaatt
     2101 gtagatatag acacaattaa gaaaactaat caatctctaa tatctactat tgatgaggta
     2161 gttaaaatac aacatgatgg tagaataaag agacaagaag cagaagttga attatcaaaa
     2221 atagaaaatg aacttaaaag taagcttctt gaatttacag ttaaataaaa attatactaa
     2281 tataaaaaga gactgtctca aaatagagat aagtctcttt ttcattgttt aaatgattaa
     2341 agtatattta tattataaat ttctattatc ttttaacata acatcatgag ctccaccttc
     2401 aactatacta gtagaagata caagtgtaag tttagcattt ttttgaagtt caggaatgct
     2461 aagtgctcca cagttacaca tagtagaacg aactttactt aaagaaagag ttacattgtc
     2521 tttcaaagaa ccagcataag gcacataaga gtcaacacct tcttcaaaag aaagtttctt
     2581 atctccacca aggtcatatc tttgccagtt tctagcacga gcagaacctt ctccccaata
     2641 ctctttcata taactaccat ttatattaac tttgttagtt ggactctcat caaaacgaga
     2701 gaagtatcta ccaagcatta taaaatctgc acccatagct aaagctaaag ttatatgatg
     2761 gtcataaacg ataccaccat cagagcaaat tggaatataa atacctgttt tttcaaagta
     2821 ttcgtctcta gcttgagcaa cttcaattat agaagtagct tgtccacgtc ctataccttt
     2881 ttgttcacga gttatacata tagaaccacc accaatacca actttaacga agtcagcacc
     2941 agcttcagct aggtaaagga atccctcgcg gtcaactaca tttccagctc ctaccttaac
     3001 tgtatcacca tatttttcac gaatgaaatc aagtgttatt ttttgccact cagaaaaacc
     3061 ttctgaagaa tctatacata aaacatcagc accagcttca actaaagcag gaacacgttc
     3121 agcaaagtct ctagtattaa taccagctcc aactacatat ctcttagaag aatctaaaag
     3181 ttcaagtgga ttttctttat gagaagagta atctttacgg aataccatat acataagatt
     3241 accattatca tcaagtacag gaagagaatt aagtttgtgg tcccatataa tgttgtttgc
     3301 ttcctttaaa gtaacatctt tgttagcata aactatagat gacataggag tcataaattc
     3361 actaacttta gtatctaaat ccatacgact tatacgataa tcacgacttg caacaatacc
     3421 caataattta ccagttgaag ttccatcttc agtaacagct actgtagaat gtccagtttt
     3481 ttcttttaag tcaagtatgt cttttaatgt gttatcaggt ttaatatttg aatcacttac
     3541 aacaaaacca gctttgtggg atttaacacg tgctaccata gcagcttcat tttcaatagt
     3601 ttgagaaccg tatataaatg aaataccacc ttctttagca agtgcaatag ccattttgtc
     3661 atcagaaaca gattgcataa tagcagaagt caaaggtata ttcatataaa tatctgcttc
     3721 ctcgcccttt ttaaatttag taacaggtgt ttttaaactt acattagcag gtatacaatc
     3781 agtagaagag aaacctggta caagaaggta ttcactgaaa gtatgtgatg gtgtatcaaa
     3841 ataaaacgcc attttttaat cactccttat atttttatac tttttataat tgatagtatt
     3901 aattataact tattctttaa ttttattaaa gtattttgtc aaatattgag atttttgaaa
     3961 aatttaaac
//
LOCUS       DAEPLF010000266         3939 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEPLF010000266 DAEPLF010000000
VERSION     DAEPLF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.267"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..239
                     /locus_tag="KR314_003327"
     CDS             <1..239
                     /locus_tag="KR314_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005209661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329684.1"
                     /translation="VEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            497..1546
                     /gene="carA"
                     /locus_tag="KR314_003328"
     CDS             497..1546
                     /gene="carA"
                     /locus_tag="KR314_003328"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBH0329685.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            1739..>3939
                     /gene="carB"
                     /locus_tag="KR314_003329"
     CDS             1739..>3939
                     /gene="carB"
                     /locus_tag="KR314_003329"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBH0329686.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYL"
BASE COUNT         1630 a          400 c          777 g         1132 t
ORIGIN      
        1 gagtagaaga atctagacca aacatattag atgctataag aaataaacaa gttgatatag
       61 taataaatac accaactaaa ggaaatgact ctacaagaga tggatttaag ataagaagaa
      121 cagctattga attttctact gaaataatga catctttgga tacactaaaa gctttagtag
      181 aagttaagaa gaaacattta aataaagatg agttaaaagt ttacaatata gctgaataga
      241 aattaagttg attgtatagt ttaagaattt aattattaaa taaaaaaaaa gaggttattt
      301 tttctaggag tttttacttt tgagataact tctttttttt aagaaatttg tagtataata
      361 gaaaaagata aaaaataaat gtaaataagt ataaaactag aatgtaaaag tgaaaaatag
      421 atatagatat ttaaaagctt atatttagtt aatgttcttt agtttgtcta aagagaatta
      481 taggaggtac tgtagtatga aagcaaggtt aatattagaa gatggaacag tttttatagg
      541 aaaggcattt ggatatttag aagaaagtgt aggagaagta gtatttaata cttcgatgat
      601 aggatatggt gaagttttaa cagacccttc ttactatggt caaatagtta ctatgactta
      661 tcctcttgta ggaaattatg gtataaattt atcatcagca gagtctgaaa aagttcaagt
      721 aaagggattc atagtaaggg agaaaagtga ttctccaagt aattttagat gtgaaattga
      781 tatagaccaa tatttaaagc aaaataaagt tatcggactt gaaggaatag atacaagagc
      841 tttgactaag atacttagaa ataatggaac aatgaaaggt ataattacat tagaagacag
      901 taaattagaa gatgttaaac ataaattgga taagttttca aatacagaag cagttagaac
      961 tgttacaaga aaagaagtag agcatataaa aggaaatggt ccaaaagtgg ctgttatgga
     1021 ttttggagta aaaagaaata tattaagatc atttatagct cgtggatgtg atataacaat
     1081 atttccagct acaacatctc cagaagatgt attgagtata aaccctgact taatattctt
     1141 gtcaaatgga cctggagacc cagaagacct agaagatgtt atagaaaata taaaagctct
     1201 tattggtaaa aaacctatag ttggaatatg cttaggacat caacttttag ctttagctct
     1261 tggaggaaaa actgctaaac ttaaatttgg gcatagaggt ggaaatcatc cagttaaaga
     1321 tttagaagaa ggaaaagtat ttataacttc tcaaaatcat ggttattatg tttcagaagt
     1381 tcctgaacaa atgaaagtga cacatataaa tttaaatgat aatactgttg aaggaatgag
     1441 acatgagaag ctagatgtat acagtgttca ataccatcct gaagcttgcc caggaccaaa
     1501 agataatgac tatatttttg ataaattttt agaattagta aaataaataa cattagaata
     1561 gctaactaaa aaacaagaat aacttaaatc aaaaagtttg gtttaattaa catagatgta
     1621 ttaaatagat gtattgatga gtaaatttga acttaagata aaataaagta attaaagttg
     1681 attgtataga tgatgaagat aagaactgtg aaaattgttt agataaggag aaagagttat
     1741 gcctaaatta gatagtataa agaaaacttt agtattaggg tcagggccaa taataatagg
     1801 tcaagctgca gagtttgact attcaggaac acaggcctgc caagcattaa aagaagaggg
     1861 aattgaagtt gtattagtaa atagtaaccc agctactata atgactgata aagaaattgc
     1921 agataaaata tatatagaac cattaacaat agaatttata gaaaaaataa tagaaaaaga
     1981 aagaccagac agtttacttg caggtatggg tggtcagaca ggattaaact tagctgtaga
     2041 attacatgat gctggtatat tagataaata taatgtaaaa gttataggaa catctataga
     2101 atctataaaa aagggcgaag atagagattt atttagagaa gtaatgaaag aaataaatca
     2161 accagtaata gttagtgata tagttacaaa tttagaagca ggtcttgagt ttgccaataa
     2221 gataggatat ccagtagttg taagaccagc atatacgcta ggtggaactg gtggaggtat
     2281 agctgatact gaggaagaat taagagaaat actttcacat ggactacagc taagtcctgt
     2341 tggacaagtt cttcttgaaa agagtataaa aggttggaaa gaaatagaat atgaagtaat
     2401 gagagatgga aatggaaact gtataactgt atgtaatatg gaaaatgtag acccagttgg
     2461 agttcataca ggagacagta tagttgttgc tccaagtcaa actctaagtg atgaagagta
     2521 tcaactactt agaaaggcat caatagacat aataaatgca atagaggttc aaggtggatg
     2581 taacgttcaa atagctttaa acccacatag tttagaatat gcgataatag aaataaaccc
     2641 aagagttagt aggtcatcag ccctagcatc aaaagctaca ggatacccaa tagcaaaagt
     2701 agcagctaag atagctttag gttatacttt ggatgagata gaaaatgcag taactaaaaa
     2761 aacttatgct tgttttgagc caacattaga ttatgtggta gtaaaaatac caaagtggcc
     2821 atttgataaa tttagacatg caaatagaaa attaggaact aagatgatgg caactggtga
     2881 aatcatgagt ataggaagta actttgaggc tgctattctt aaagggataa gatcacttga
     2941 aacaggaaaa tattcattgg ttcatgctcc atcagaaggt agaacattag aagaattaaa
     3001 ggctagagta gtagtacctg atgatgagag actatttgac ttagctgaaa tgataagaag
     3061 aggctataaa gtagaaatga tatcagaaat aactggtgtt gataagtggt ttataaataa
     3121 atttaaatgg atagtagaac aagaagaaaa gttaaaagtt ctaaaaatag aagacttaga
     3181 taaagaatat ttacatgaat taaagaaaaa gggattttct gataaaggta tatctgactt
     3241 aatgaagata agcccagaaa aattatatga attgagaagt ttatataata tacaaccagt
     3301 ttataaaatg gttgatacat gtggaggaga atttgaagct ctatcacctt attactattc
     3361 aacttatgaa caatatgatg aggtagtagt tagtgataag agaaaagttg tagtattggg
     3421 ttctggtcca ataagaatag gtcaaggtat agaatttgac tattgttcag ttcactgtgt
     3481 aaaatcttta agaaaaatgg gaatagaaac tataattgta aacaataacc ctgaaactgt
     3541 aagtacggat tttgatacat ctgataagtt gtattttgaa ccattaacag aagaagaagt
     3601 tttaaatata atagaaaaag aaaaaccaga aggtgtaata ctacagtttg gtggacaaac
     3661 agctataaaa ttggctaagt ttttacatga aaaaaatata cctatactgg gaacagactt
     3721 tagagatata gatgcagcag aagatagaga aaaatttgat gatttactag agagactaga
     3781 tataaataga ccaaaaggaa aaggtgtatg gacaactaat gaaggtgttg aaattgctaa
     3841 ggagttaggt tatccagtat tagttagacc atcttatgta cttggtggtc aaggtatgga
     3901 aataacttat aacgaagaaa aactaattca gtatttgga
//
LOCUS       DAEPLF010000267         3912 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEPLF010000267 DAEPLF010000000
VERSION     DAEPLF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.268"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(3..863)
                     /locus_tag="KR314_003330"
     CDS             complement(3..863)
                     /locus_tag="KR314_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0329687.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            1012..1902
                     /locus_tag="KR314_003331"
     CDS             1012..1902
                     /locus_tag="KR314_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0329688.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLSPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            2120..3874
                     /locus_tag="KR314_003332"
     CDS             2120..3874
                     /locus_tag="KR314_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBH0329689.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDVTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNVKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVSVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLVGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         1591 a          419 c          626 g         1276 t
ORIGIN      
        1 agttattcta tttttttaaa tccattacct aaagcttcat gtatatctgt aattataata
       61 aaagcattag ggtcgacatt ttttactaat ttttttaatt cgacaacttc tttcttttca
      121 actactacta aaagtacatt tttattttct ccagtatatg caccttctcc ttttagaata
      181 gttgcacctc tatctaaatc ttccattatt tttttactta tatcttttga aaggtcagat
      241 ataatagtga atgatttgga ataattaaat ccttcaataa gcaagtcact aacttttact
      301 aaaatgtata aagccacagt tgaataaagt gcaatttcaa cctgtctatt tactatacct
      361 gataaaacaa ctacaacgca gtctacaata cccattaaaa caggtatact aaatgtagga
      421 aaatatttat ttagtaggag tgccattaaa tcagttccac ctgtagagcc atttattcta
      481 aaaataagtc cttgaccaac tcccattaaa atagaaccag atataattgc aagatacata
      541 tcatttgtta tatctaatgt agccatattt gcagtaagct taagtgctat ggtaagaaaa
      601 attattccta aaaccgtttt aaaacaatct ttttttgaaa gtattttgta agcaaatatg
      661 aataatggaa catttaaaag taaattgaca atccaaagtt ctacaccgaa taatgaattt
      721 aaaacaactc caagtcctgt tataccacca gcagcgattg catgtggatt aaaaaataaa
      781 ttaagagaaa tggccattaa tatacagcct acaattaata ttaaaatttc tgaaaaagtt
      841 attctatctt ttttcaataa catcactcct tatatataga ttataatata tattatatag
      901 tttttccaat ttttaataaa agaaataaaa atatattata aaaacaattt gattggaatt
      961 ttcagaaaat taatgctata attaaaagta aatttaaagg gaggttataa aatggctaaa
     1021 ttaactctag aacatataaa aggaaataca tattatattc cactccctac aatagtaggt
     1081 gtatacgtag atggtaaaga tgcaatttta attgacagtg gaaataataa ggatacagct
     1141 agacaagttt taagattatt agaagaacat aatttaagtc ctaaattaat aataaacact
     1201 cattcaaatg cagaccatat aggaggaaat gcatatttga agaatcaaac aaaatgcaag
     1261 attgctacga ctaaaattga aggatatttt acagaaaatc caatattaga atcagcattt
     1321 ttatatggtg gctatccaag taaagcactg aagaataaat ttttattagc aaaggaaagt
     1381 gaagttgatt atattatacc ttcaaatggc aaaattgtag atacagaact tgaagcgatt
     1441 tctcttccag gtcattattt tgaaatgatt ggtgttagaa ctccagataa tgttttgttt
     1501 gttgcagata gtcttacacc agagaatata attactaaat atcatttttt cttcctactg
     1561 gatatagatt cacagtttaa gacactagaa aaattgagaa tgttagaggc tgatttcttt
     1621 gttccaagtc actctgtcaa aactacagat ataaaaagtt tgattaatat taataagaag
     1681 aagatggaag aaataataga taatattaaa aaggtttgtt atgagccagt tatgattgat
     1741 gaagttattg aaaaaatgtg tgatttgtac aatgtcaagc ttgatgctaa tcaatatgtg
     1801 ttggttggaa gtacaatacg ttcttatata acttatttat atgagaataa tatggttgaa
     1861 tatatatttg atggtggtaa gatgatgata aaagctgtgt aaatagtaat aattttcaaa
     1921 ttatatgatg aaattgtttt agtatgactg ttatttgaaa agtaacataa aaaaatttat
     1981 ataaataagc taagatgatt gatataaagt tactgataat tttaaggtaa tatatacgtt
     2041 actataatat tatgttatta tttgtaaata aggggataat ttataagcaa attaaagacc
     2101 ttaatttaca ggaggaatag tgtatattat gaataaaaaa ttcaaaaaat tcagtaaaaa
     2161 actaagtata ttaattttag ttttgactat gtttcttttt aataatttaa ctgcaaatgc
     2221 acagattaaa gaagattcaa taataggaat tgatagatat gaaacagcag gacttatagc
     2281 agatagacaa acttatgata ctgtaatatt agttaatgga gataaaaatt tatctgatgg
     2341 gctttcatca agtggattag ctggagctat taacgcacca atacttttga ctaaaaaaag
     2401 tgaaatacca aaagctactt taaatagact agataataag agtttaaatg ttgtaaaaaa
     2461 agtatatata attggtggtt ataatactat tgaaaattca gtagaaaaag atataaaaag
     2521 aaaaggtatt caagtggaaa gaataaatgg taacaataga attgaaacta gttataatgt
     2581 agctaaaaaa ataaatgaag taagtagtgt aaaagaggta atgcttacaa atggatttgt
     2641 aggagaggct gatgctatga gtatagcacc tgtagttgca aaaaataaag gagctataat
     2701 acttacagat gggaaatcag ttccatttaa gactgaaggt ttaaatgttt acgctatagg
     2761 tggcaaatct gcgataaatg aggatttagt aaatgaaact aaagcaacta gaatcggagg
     2821 aaatgataga tttgagacca ataaaaaggt aatggaaaag ttttataatg gagcgacaga
     2881 tttttatata acaaatggat atcaattagt agatgcacta acattatctc ctttagccaa
     2941 agaaaaacca atcgttttag ttgctgatgg aagtaataaa ggtattttaa aaggagctaa
     3001 aagtataaca aaagttggag gtattgatgc caatatatat aaacagtgtt tagatatgat
     3061 taaatataat aatatggatg tgactccaaa tattttaaaa cacttaactt cagttgaagg
     3121 aaatgaagtt tctgaaatac ttatagaatc agataattta ggtgtggttg ttgaaaagac
     3181 gaataccaat aaatttgagt ttgattacgt aagtgtaact aatgaaaaaa attgtacttt
     3241 ttctgtatat aaagaaaatt cttctaataa tgtaaaaaat ggaaagctag ttgtaagtgc
     3301 taagaaaaaa atagataaga aagatagtga tttgcaagat atgaatggcg acaatatgat
     3361 aaatgcaaat aaagatagga tggtaaatgt gataaaaatt ggagttcccg ataaaatgta
     3421 ttctaatttt aaagtagatg ttgaaagtgg ttcagtagaa ctttataata ttaaaggagg
     3481 agctactgtt gatgtcaatg acggaattgc taacattgtt gataacaatg tgacaaatcc
     3541 tttcaatata aatactaata gtgggatttc aggtgtaaca gctgaaacta tatcatctga
     3601 gataaaattt agagcaaaca atggaattgt aagtgtaaca gctacaaata taaatggcaa
     3661 tataagttta tttggaagcg ataataaagg tacctttgat ggaatattta aattaaatct
     3721 gaaaaaagaa ccatcaaatt tacacttgaa gttagttgga aatgggataa ataaattgcc
     3781 agaaggttgg tctaaagatt atattttagg aaatggacaa ccaattattg aagttaaaaa
     3841 taatggcata aataatatat caattggaga ataaaaacat atgaaaataa agtttatata
     3901 gttttaaaaa tt
//
LOCUS       DAEPLF010000268         3905 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEPLF010000268 DAEPLF010000000
VERSION     DAEPLF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.269"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..819)
                     /locus_tag="KR314_003333"
     CDS             complement(<1..819)
                     /locus_tag="KR314_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH0329690.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKAGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQD"
     gene            1114..1848
                     /locus_tag="KR314_003334"
     CDS             1114..1848
                     /locus_tag="KR314_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0329691.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            1858..2991
                     /gene="nagA"
                     /locus_tag="KR314_003335"
     CDS             1858..2991
                     /gene="nagA"
                     /locus_tag="KR314_003335"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBH0329692.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNITHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            3090..3839
                     /gene="nagB"
                     /locus_tag="KR314_003336"
     CDS             3090..3839
                     /gene="nagB"
                     /locus_tag="KR314_003336"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBH0329693.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKLENTHLP
                     NGMADDIEKECLNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
BASE COUNT         1535 a          469 c          637 g         1264 t
ORIGIN      
        1 atcttgataa attaatcctt ttttcataag cactccaaga gctcgtttaa aatttttctt
       61 acttgaatta aataatatag aaatatcttt agggtcagat ttatcattaa atctcatata
      121 tccatcagaa ccctctaaat aacttaaaat cttattttct aaagtatcaa gttcatcctt
      181 tctatttcct ctaggagaaa gaccaagttt tccatcttca tatattttta tgacatgtaa
      241 attttcaaga acatcaccag cttttagttc agtaaagtac tcattatgaa gtattacacc
      301 agcatattta ttgtctacac atatcattgc agaattatta gtttgaaatc catatacaac
      361 tccagttaca atatctccta cattgtaagt atggtctgtt aataaatatg aatctatatc
      421 tgtagtagca gcaattcttt tgcttttgtc taaatatata taaaataaat atttttcgtc
      481 tctaaatagt ggataagtct tagctttaaa aggaactagt atgtctttag gaagtcccat
      541 atctataaaa gtaccaatgt ccgtattatc aactactttt aaatgtgcta catccccaac
      601 cttagctaaa ggcggtatta aagttgcagc agttctgtca tcagaatctt taaatatgaa
      661 agcatttact tcatcaccta tgtttatttc attttttccg attaatctat tatgaagaag
      721 tatatcgtct tttgtgttat tagtttgtcc atccaaaaaa tatccaaatt cagtttttct
      781 ttttactgta agtttattaa aatcacctat gtttatcaaa ttaaaatcat cctctcattt
      841 tatatttata atttagccat aatatactta tgtagtagtt tgtcacagta tctatattag
      901 tattagtgaa atttacaata aataatatag ttatttatgt atttttcaat tctctagctt
      961 cttatatagt ataaactcca atttcttttt tgtctttagg ttttactaaa atttttacaa
     1021 aataagttaa aaagtattga aataaaattt cttttaaagt ataattggta tataccaaaa
     1081 ttggtatata ccatatcgaa aagtggtgac tatatgaaaa tagtaaataa atctagtaat
     1141 ataccattgc atactcaatt aagttcaata ataagagaga tgatagaaac tggtgagctt
     1201 aaagagggtg atgcaataat gccagaaaga gaactttgca acatacaaaa tgtaagtcgt
     1261 atgacagtaa ataaaaccat tgttggatta gtcacagaag gacttcttta tagggtacaa
     1321 ggtaaaggga cattcgttgc aaagcaaaaa aagaagtatc agttttcaaa tgtaaaaggt
     1381 tttactgatg ttatgaaaga aaagggagta aacataaaaa cggatatatt atcctttgag
     1441 atggaactac cagatgattt agttaaaaga aagcttggaa taagtgataa tactacaaat
     1501 atatataaga ttgtcagact tagatatact gatggagagc catttggatt agaaatagta
     1561 tatctatcag aagaaatgtg taaaggtcta acaaaaggga tattggataa ttcttcatta
     1621 tatagggttt taaatgaaaa atatggctat aagatacaaa aagctgaaca agtaatggag
     1681 ccagtaatac tttcagatga agaaagtaag cttcttgaaa cagatgaagg tgctttagct
     1741 cttaaacttc atagaaattc ctataataga gaaggtagcc ctatagaata taccatatca
     1801 atatttagaa cagataaata tcaatatgaa atagtattga gtgagtaggt gaataatatg
     1861 aaatgtttaa ttaatggaaa gataatatta aaaaatcaaa tcttggaaaa taaagtatta
     1921 gtatttgatg aaaaaataat tgatatagct gatagtgtac caaaagattg tgaagttata
     1981 gatgctgatg gaaaatatat atctccaggg cttattgata ttcatataca tggaaatatg
     2041 ggaaaagata caatggattc gactgatgaa tctattgaaa caatttcaaa atcaattatg
     2101 agacatggag taacatcttt tcttccaact acaatgacta tggataaaga acatgtttat
     2161 gatgcacttg aagttattaa aaaagcccaa aatagaaagc ttgaaggggc acaagtatta
     2221 ggagcacatt tagaaggacc ttttataaat gagaattata agggagcaca aaatgaaaaa
     2281 tttataataa attcaaaata cgaatttata aaagaatata aagatgttat aaaagttata
     2341 acttatgctc cagaaaaaga tattgatttt gattttacta gagaaataaa aagatgtaca
     2401 gatatagttt tatctattgg tcatagtaat gcaaattatg accaagctaa agaagctata
     2461 aatttaggag taactaatat aactcacatg ttcaatgcaa tgactggttt aaaccataga
     2521 gacccaggcg ttgttggtgc agcacttaca actaatgtgt acagtgagct aatagcagat
     2581 acaatccata taaataaaga tttatttcaa tttatactaa ataataaagg gaaagaaaga
     2641 ctgattttaa taacagattc tatagaagct ggtggtcttg aagatggcaa ttattctctt
     2701 ggaggtcaga aagtcatagt taagggtaat gaagctagac ttgaaaatgg agcattagct
     2761 ggaagtgtgt tgtctttaaa taaaatggta tttaacttct tagataatac aaaccttaaa
     2821 gtgaatgaag caattaattt agcttcttta aatccagcaa catctttggg aataaatgac
     2881 aaaaaaggaa gcttagaaat aggtaaggat gcagatatag ctgtatttga tgagaattta
     2941 gattgtaaaa tgacactttg cttgggggac gtagtatata aaaatatata gtatacaaaa
     3001 caaactttaa agacagtaaa aacaatataa aaaataataa attaaactta cattagttaa
     3061 agtagtagtt aaaatttagg aggaataaga tgagagtatt agtgtgtaaa gattatgatg
     3121 gaatgagtaa aaaagccgct gaaatgatag cagctcaaat agttttaaaa ccaaattcaa
     3181 tactgggact agctacagga agtacaccag taggtatgta tagagattta gtaaaaaaat
     3241 acaatgataa tatagtagat ttttcagatg tgatgagctt taatttagat gaatattata
     3301 agttaccaat atctaatgac caaagctatg attattttat gaaagagaat ctatttaatc
     3361 atgtaaacat aaagctagaa aatactcatc taccaaatgg aatggctgat gatattgaaa
     3421 aagagtgtct gaactatgaa gcttctatag atgcagctgg tggtatagat gtacaagtac
     3481 ttggaatagg tcgtaatgct catataggtt ttaatgaacc tgatactaaa tttgcaaaaa
     3541 gaactcatgt agttgaactt acagagtcta caatagaagc taatgcaaga ttctttaaat
     3601 caagagaaga tgttcctaaa aaagctgtaa gtatgggaat aggttcaata ttgaagtcta
     3661 agaaaatatt gcttttagct tcaggagaag aaaaagctga tgcagtatat aatactgttt
     3721 acggagatat aactccagaa gttcctggtt ctatacttca acttcataaa gacactatag
     3781 ttatagtaga tgaagcagca gcaagtaaac tgaatccaaa agattataaa ttagtataag
     3841 aatagaatgt agtttaagtg aaaaatagct atcattgaat aatcaaattt atttaatgat
     3901 agcta
//
LOCUS       DAEPLF010000269         3876 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEPLF010000269 DAEPLF010000000
VERSION     DAEPLF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.270"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(602..853)
                     /locus_tag="KR314_003337"
     CDS             complement(602..853)
                     /locus_tag="KR314_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329694.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(1099..1254)
                     /locus_tag="KR314_003338"
     CDS             complement(1099..1254)
                     /locus_tag="KR314_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBH0329695.1"
                     /translation="MALDVKIEVKIIKDVVILNIIHWKKETSLLTVKIARGQGDLSYV
                     KYFTNES"
     repeat_region   2471..3424
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2471..2499
                     /rpt_unit_seq="ctttacataccacttagttaatataaaac"
BASE COUNT         1345 a          587 c          356 g         1588 t
ORIGIN      
        1 atttaatata tgtacactat tcatatctta atagtcaata ttattcattt ttaatagctc
       61 tttttagatg tcagaattat tatattttga aatttattaa atttttgcat taaaaaaact
      121 agaaattaat ttaagtcttg aattacttat aactttctac gtatctacct ttatattctt
      181 actctgattt tatatctaat attaactctt catcaaagct attaaatata cctctaattg
      241 agtcaattat ttcacttact tcacctttaa tttttatatt aacttcaacc atttcaaatc
      301 tcatcttttt atcttataat ttctataagt ttttttattt attgtgttgc ttactttgac
      361 cagcactttt ttatttaata gtctaaatga tattttttac cagttttaat atccttaaat
      421 actatattta ctaaaaactt ataatttctt ttaatacatt cttattacta ataacaatac
      481 tcaacaattt tatcgccttt tttcaaatat gatttaactt ttttattcct aattatttaa
      541 aaatagttgt ccaattttct tctatgagaa agtgctaagt attagcaact tctctattct
      601 cttaactttc tactgtctca actttcttca tagctatatt aatagctact tctttagcta
      661 cttcattgaa ctcttcccat cttctcctat tttcttcatc agatatacgt ggatatacta
      721 tataaacttc tgtgttttga ttagctataa ttttttcatc atattctata tttttttctt
      781 tatcatattt gtatggttta aattgttgta gtattttcat aacatcaccc cttgttataa
      841 aatatgaact cataaacttg tcttatactt atttaattgt tacaattttt acttaaaatc
      901 actttatatt ctatatttta aagctaaatc ctttacaaaa gtaacatatt cttctataag
      961 tttcttatcc tcttatatag tatctagttt gtttaactta tctcttttaa acttacatac
     1021 gccttcttct gccattcgtc ttttattaat tgttaacatt gtttcaagag acacaccaaa
     1081 actcttgtta agaagctctt agctctcatt agtaaaatat tttacataac ttaaatcacc
     1141 ttgacctcta gcaattttaa ctgttaatag acttgtttct tttttccaat gtattatatt
     1201 taatataaca acatctttta ttattttaac ttctattttt acatctaatg ccatattatt
     1261 gctttattaa tttgtatcct taaatcttaa attttctatt aatacatctt ctatacagct
     1321 tagtttctct cgcttaatct tgaaatatat tttctctaaa ttgtcaaatt gttcccaagt
     1381 tttatcaata tctaatattt tgcagtgtct atttgcacat ctttcgatcc aaaggtataa
     1441 ttgactagaa tgttttgaaa tcaactgact attagtaggt tagttcttaa attctttcaa
     1501 atactcgaat tgtaataaaa cataatttac attctcttta tttctattga agttagttta
     1561 tatattgttt acatcatttt catatatatc tgctagttgc tatgcattta aaattctctt
     1621 attatttctt tatattactt gtaaattatt catcctcatc atcctcttaa ttaaatttct
     1681 atcttagaaa tttaatttgt aaaaaaattt tctatatcat aatcaagtat cttagatagt
     1741 ttataggtaa catatcaact ctaattgaat attacccctt cctgtactta aaataagttg
     1801 atgcattttt aaaacctaac tcttttgcaa cactttgtaa gtataaattt tatgtatgtc
     1861 ttctttttta taaaatcata gtgtatcttt ttcatatatt caccttaatt gctatatttc
     1921 aataacaata tttccaaaac agaaattttt cgaggtaatt atgtatagat gtaaatcaaa
     1981 gaattaaata tactagggaa aatatgaata gttctcaaaa agaattgctt gaaaaaacaa
     2041 atataaatac aagtgttatg aatagaatct ggtgaaagac caacaagaga cgatgggtta
     2101 ataatatttg ctaaaatttt tggtttatat actgattatg ttcttggttt atcagataca
     2161 gaaaaactaa atattggcaa ttaaaactga attgattgta ttatatgtgt gatgaatata
     2221 aatatagaat tttttgagaa gcttgaaata gcttagaaaa aataagagat tcactcaaga
     2281 ataagttact aaagattaaa aaataacaaa agtatctact aaaggatatg aaaatggttt
     2341 aagagagtta agtctggaaa tatttatgtg tattctttaa ataaaaattt attttttaaa
     2401 tgataattaa atttttactg atatggctat gcttttaaaa gtcataacct tattttgata
     2461 ttagatctgt ctttacatac cacttagtta atataaaact aaatatagct tgtaactcct
     2521 ttgatgcact taaaagcttt acataccact tagttaatat aaaacgaatt tagctataga
     2581 agcatcaaat tgtggcacag cactttacat accacttagt taatataaaa cctttaaaga
     2641 caaaacgtcg tataattaaa tcatagcaac tttacatacc acttagttaa tataaaacct
     2701 tataattctt taagtactag actgagcaat ttaatcttta cataccactt agttaatata
     2761 aaaccatccc attcagcaac aacagtcgct aaatcttctg cctttacata ccacttagtt
     2821 aatataaaac ttttagtata ttttttaggt ctgccctctt taaaactctt tacataccac
     2881 ttagttaata taaaacacga ggttatagtt aattttgtcg tgggagttta taactttaca
     2941 taccacttag ttaatataaa acaatttata tatatatata tattaaagtc agaccccttt
     3001 acataccact tagttaatat aaaacttgtt gttcagcatt agcaatcata tcatctattc
     3061 ctttctttac ataccactta gttaatataa aacctgtacc atcagagaca gtcattaaaa
     3121 gatttataaa ctttacatac cacttagtta atataaaact tataaattgt ctctgtaccc
     3181 attttattca atgtcgtact ttacatacca cttagttaat ataaaaccat gaaaaaatgg
     3241 gtaattcaaa ctcttttatt ttctttacat accacttagt taatataaaa cgttggagaa
     3301 aataatcctt cttatgattt gccaagcggc tttacatacc acttagttaa tataaaacca
     3361 gcggatgaat tatttatgtt aattggagat ggagacttta cataccactt agttaatata
     3421 aaaccccaaa ataaattgag tatttccaac acttacatct atacaattct ctcaaatttg
     3481 cagtgaacca tgagtagtgc aaatgataac atttatcaca taccctcaat gccttatatt
     3541 tcaactgtta ggccctattt tatcaaaaat atcgctcact gcaaaactat ttataatttt
     3601 ataatattat gaatatatta tttttaaata tctgtaccaa tttgtagtat aataaaatca
     3661 agaaaataat ttactcctat aaagagtgat tatttttaag tgaaaatatt atttttttaa
     3721 atcatcctta ttggcgtttg ggagattttt attttatcat aaatataaac tgatataaca
     3781 ccagctgaca tgcttgctgc tttcgattat tattcttgct acaattatta taacatataa
     3841 aacttactta tttttaccaa atattttata caaaca
//
LOCUS       DAEPLF010000270         3874 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEPLF010000270 DAEPLF010000000
VERSION     DAEPLF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.271"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            66..482
                     /locus_tag="KR314_003339"
     CDS             66..482
                     /locus_tag="KR314_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329696.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(664..1233)
                     /locus_tag="KR314_003340"
     CDS             complement(664..1233)
                     /locus_tag="KR314_003340"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBH0329697.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            1387..2544
                     /locus_tag="KR314_003341"
     CDS             1387..2544
                     /locus_tag="KR314_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBH0329698.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            2731..>3874
                     /locus_tag="KR314_003342"
     CDS             2731..>3874
                     /locus_tag="KR314_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBH0329699.1"
                     /translation="MFNKNEILKKLIEEYDVKTTTDIQDMLKDLFASTIHQMLEAELD
                     DHLGYDRYDNKNKNTKNSRNRYRNKNVKSDFGEITLNIPRDRNGDFEPKVIRNYENDI
                     SGIENQVIGMYSKGMSTRDISSHLKSIYGIDISHSLIAKITDRVLPLAHEWQSRALDM
                     VYPIVFMGAIHYKVRSEGRIINKAAYIAIGVNLEGITEVLGIWIGENESSKYWLKVLT
                     DVKNRGVKNILITSVDGLVGFSDAIKAVFPDTEIQRCVVHQIRNTLNYVSYKNRKEFA
                     KDLKKVYAAITEEVALSELSLLEEKWGNQYAIALRSWRSNWNELATYFKYPQEIRTLI
                     YTTNSMESYNRLLRKVTKSKSIFPSDDSLHKSLYLATMDISERWTQK"
BASE COUNT         1519 a          403 c          690 g         1262 t
ORIGIN      
        1 ataatttaga gtctaagata agcttataaa tcatatattt agaatatatg ggaggagtga
       61 taagattgaa tgtaaaattt aatattaaag gtataattta tggagttata gctcttgtat
      121 tcgttatagg gggatttata ttcataccaa gtatgtttgt agagaaatct aaacccatag
      181 attatacagt attacaaaga aattcaatac cagaaaagat attagatgtg atggataagt
      241 atacaaatga agaaagagca ttagcggcta aaatcgataa caaaatatac attatagtga
      301 ctagaggaaa taataaatat ggtatagaaa tggacaaaat agaaagtgtg gttgaggaag
      361 gaaaggatgt tttgagagtc aagataaagt ataaagataa ggaagattct tttccataca
      421 tagttgttga aacaaatatg agtgaacttc cagatagaat agaattaaat gcgacgaaat
      481 aagaaggtat tttataatta aataactgca tattgtacaa taaagtcatg gtttttatta
      541 atgtaagtaa attgtatatg gttcttattg ttgtaaaagc catgacttaa aatgcttata
      601 aagtaagata ttaacagtag tatttattac tatattaaat ggggtctgga aatacttaat
      661 gttctaatta tttggagtga taattctagt ttcagtaact atgtagtcta attgtgcatc
      721 atgagtttct tttggaatag aatcgaatat ctggaagtca aatgctatgc caatagttat
      781 agcatctttt cttaagtttt ctaaaaatct atcataaaat cctccaccat atcctaatct
      841 atagcaatgt atatcatatg ctacagctgg tacaattaca acatctaagg ccttaatatc
      901 aattgtaggt gatttttcgt taggttctct tattccatat gctccaattc tcaatccctc
      961 ttttaaatct gttatttgag tagggattaa tgtatgattt tcttttattg tgatgggact
     1021 tgatactatt ttctttagag acaaaagttt tttgattaaa ttatctgttt gaacttcatt
     1081 gttaaaattt agataaagca ttatattagt tgcatttctt atatcttcca tttgaagtaa
     1141 tttttcagtt attaaatttg aattactaag tacaaagttt gcactcttat tttttcttga
     1201 ttcaataact ttttttctaa attctttttt cataaaataa ttcctccctt aatatgttat
     1261 aatgtgtaca gcaattgtat ttaatataaa ttttagtata atttattgta ataaagcaat
     1321 atatttgggt aaatgtaata aaaataacag ctagtcatat aataataata gtttatgtaa
     1381 aaggagatgt atcttgtgat ttcaaagaag aaagctattt ttttaggggt gattttagta
     1441 attataacag ctatggttac atcagcattt cagttgacat tggggaataa agttgtaata
     1501 tcgaaagaac tctatgaaga ctataaaaaa tatgataagt tgttaggact agaatctatt
     1561 ataaaacaag atttttataa aaaagtatca gatactgatt tggtaaatgg agcagcaaaa
     1621 ggtctatttc taggaacaaa tgataagtat tctggttatt atactaaaga tgaaatggag
     1681 aatctaatta atgatagtga aggttcttat gttggtgtag gtatgtatat aggggcatca
     1741 aaagatggtg gcttagtagt agttcccatg aaagattctc cagcagagaa agcaggtgta
     1801 aaatcagggg ataaattagt aaaagtcaat ggaaaatctg tctcatataa aaattcagat
     1861 gaagctgttc gtatgatgaa gggtaaaaaa gggaaaacag ttgaactgac tatattaaga
     1921 gaagataaac aattgaactt taaagtaaaa acagaccaga taattgagaa gagtatagaa
     1981 agtaaagtaa ttgataatga tttaggatat atagaaatta cccaatttat atcaagtaca
     2041 tatactgact ttgataaagc tttaaaagaa ttaaaagcaa aaaatatcaa gggattagta
     2101 atagacctta gaaataatcc tggtggaatg ctagatatat gtaaagaagt tgctgatgag
     2161 ttaattggtg aaggaactat agtatataca aaagataata agggaaatac tgagtacttg
     2221 aaatctgata aagaaaaatt gggacttcca atagtagtgt taactaatgg agaaagtgct
     2281 tctgctgctg aaatattaac agctgcaatc gttgataata aagaaggtat ttctgtaggg
     2341 actacaactt ttggtaaagg gttggtacaa tcagttgtaa gattgaagga tggaacagga
     2401 tacaagttaa ctactgctca atattttaca cctaatggag attatatcaa tgaaaaaggc
     2461 ataaagccta ctattgaaga aaaagatgaa aataaacagt tagatgtagc gactaaatgg
     2521 cttagagaac agattgataa ataaagttta atttttataa acttatattg taaaataaag
     2581 aaccttaata aaaggagtgt ctcaaaatta actttttagt ttatgaggca ctcttttgtg
     2641 ggtctttaaa ataaagtgtg tattcttcta aaaagtagtg ataattaaaa tattaaatat
     2701 cactatatat aaggaaggtg cacacttttt atgtttaaca aaaacgaaat attaaaaaaa
     2761 cttattgaag aatatgatgt taaaacaact acagatattc aagatatgct aaaagattta
     2821 tttgcaagta caattcatca aatgttagaa gcggagcttg acgaccattt aggttatgat
     2881 agatatgaca ataagaataa aaatactaaa aactctagaa atagatatag aaataaaaat
     2941 gttaaatcag attttggaga aataacttta aatattccaa gagatcgaaa tggagatttt
     3001 gaaccaaagg taattagaaa ttatgaaaat gatatatcag gaatagagaa ccaagtgatt
     3061 ggaatgtact caaaaggtat gtctactcga gatatatcaa gccaccttaa gagtatttac
     3121 ggaattgata tatctcattc tctaatagct aaaattactg atagagttct acctttagct
     3181 catgaatggc aaagcagagc tcttgatatg gtttatccaa ttgtatttat gggtgctata
     3241 cattataaag ttagaagtga aggtagaata ataaacaaag ctgcatatat agccataggt
     3301 gttaacttag aaggtataac ggaggtctta ggaatatgga ttggagaaaa tgaaagttca
     3361 aagtattggc taaaggtgct tacagatgtc aaaaacagag gagttaagaa tatattaata
     3421 acttctgtag atggattagt tggattttct gatgcaatta aagcagtatt tcctgatact
     3481 gaaatacaaa gatgtgttgt acatcaaatt agaaatactt taaattatgt atcatataaa
     3541 aatagaaaag aatttgcaaa agatttaaag aaagtatatg ctgcaattac agaggaagta
     3601 gcattaagtg agctatcgct cttagaggaa aaatggggta atcaatatgc gatagcatta
     3661 agaagttggc gaagtaattg gaatgagcta gcaacatact ttaaatatcc tcaagagata
     3721 agaacattga tatatactac aaattcaatg gaaagttaca atagattatt aagaaaagtt
     3781 actaaatcga agtctatttt tccttcagat gattctttgc acaaatcatt atacttagca
     3841 actatggata tttctgaaag atggactcaa aaga
//
LOCUS       DAEPLF010000271         3858 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEPLF010000271 DAEPLF010000000
VERSION     DAEPLF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.272"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            194..862
                     /locus_tag="KR314_003343"
     CDS             194..862
                     /locus_tag="KR314_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329700.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWENDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            1021..1527
                     /locus_tag="KR314_003344"
     CDS             1021..1527
                     /locus_tag="KR314_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBH0329701.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1655..2671
                     /locus_tag="KR314_003345"
     CDS             1655..2671
                     /locus_tag="KR314_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBH0329702.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            3025..3729
                     /locus_tag="KR314_003346"
     CDS             3025..3729
                     /locus_tag="KR314_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBH0329703.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVIICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1653 a          328 c          659 g         1218 t
ORIGIN      
        1 gagcatactt tataagtatt tttatgctaa ctagaaacac ttaatttatg gcatttgtat
       61 ttgatttatt ttaatgtaaa taatttcaaa gttaatttta ttcaatatag tactataaaa
      121 gtataaataa catttaataa aaatatatat tatattaggt attttaaata aattttttaa
      181 agggtgattt tttatggaaa atataactag ttattttaca acaatactat gtgtatttat
      241 atgcttatca tctgtattta tatttactca actagctaga gtatttatta ataagaagaa
      301 gattaatcaa aaaattaaat caagaaacgg atttaggtat gatagagatt ttattgaagc
      361 cagaagagaa gaaatacata taaaggataa caataataat aaaaataaga gtaacaataa
      421 gaaattaaag gaagaaaaag tatttaaata cgataatggt gatttatata aaggtgagtt
      481 tgttgatgga aagaaaaatg gatttggaat atatattttt tcaagtaagg aaaagtatga
      541 aggattatgg aaagatgaca agatgcatgg aataggaaaa tatacttata gagatggttc
      601 aatatataca ggtgaattta aatacggatt aaaaaatggt ttgggaaaat taacatatcc
      661 caataatgac atttataagg gatatttttt agataataag aaaaatggta agggtgtatt
      721 atataaaaat gatggaaata aacaagcagg aatatgggaa aatgatgaac aatgtaaaag
      781 tttagatttt aaagatttaa ataataataa aataaataat gtttatcaaa atagattaaa
      841 taacaataaa ttaaaagttt aattattcta ataaaaaaat ctttatttat gatggaatat
      901 aggatatatc tatgttgtaa tttgtaaaat cttcctatat catataaaat taagtattga
      961 ttagtctatt acataaatat agaatataaa tatatccatt taataataag gagaagagat
     1021 atgaaagaat atgtagttgt ggatttagaa actacaggat tagatccata taaaggttgt
     1081 gaaataatag aaataggaat aacagaaata aaaaatgaac aaattgtaaa aaactattca
     1141 aggttgataa aacctaaggg gataatttct tcatttataa cagaattgac acatataagt
     1201 aatgaaatgg tagaaaatga agaaccacta gaattagttt tacctagatt tagaaaatac
     1261 ataggtgata gaactataat tgcacacaat gcgaaatttg atttaaaatt tttaaattac
     1321 tatcttagga tgttagattt agaaccaata aacaattata tatgtacagt agaattatta
     1381 aaaaaatgta aaagttataa aggaaaaaac aagaaacttg aaacagcttg tgcttattac
     1441 aacatagaga atataaatgc tcatagagct gacagtgata cattagcaac tgcaaaacta
     1501 tttttgaaaa taaaagatga atattaatta taaatataga gaaaattagt gaaaatatcc
     1561 tttataaaat tgacccttaa gcttacaatt ttgttctttg atttattttg tagaacaatg
     1621 tataatgtac cttatatgaa gaagggagac taaaatgggt tattacaatg ataatgataa
     1681 taataaaata agacgtaaaa aaattagtaa caacaaagat agtaataaaa aatctaaaca
     1741 ttcttatact tcggcaaata aaaaaaataa aagtcctaat acaagttcca gtaaaagaat
     1801 gagtgaaaaa gtagattcac agaatcttat aaaaagagat agagatagtt ttacaagaga
     1861 taatgtaaga cctttaaata cagattacaa tcaaagagaa agaagaagag tcgagtataa
     1921 acaaagagag aaaaaattaa aaagtaaaaa aagaaaaaaa caaaaaagga ttagaatagt
     1981 tagggcatta ttatctatta tgttagttgc actaattata ttttcagtgg caggtgcaat
     2041 atttacagtt tcagctataa aagggtcacc agaagttact aaaaagttaa ttaggtctaa
     2101 atatataagt agtgaggttg taagtataaa acaaatgcca aatgatttaa aacatgcaat
     2161 agtttccata gaagatgaga gattttataa gcataaagga gtagatgtta tttctctagt
     2221 taggtcagta ttaaacaatg tgtttacaga tacaacacaa ggtgcaagta ctatagatat
     2281 gcaagtttca aaaaacctgc ttactagtaa tgataaaact atgaagcgga aaataaggga
     2341 tatgtataat gctaagcaga tgaataagat tatgactaag gatgaaattc ttgaggcata
     2401 tttaaataat atgtatctag gtaaaaatgc atatggagct gcaaagggag ctgaagtcta
     2461 ctttggtaaa aaagttagtg aattaaattt agctgagtgt gctatgttag ctggtatgac
     2521 aaataatcct gcaaggtata ttgaccatgg agaggctaag aagagacaag tgattgtact
     2581 gtataagatg catcagcttg gttatataac tgataaggag tatagaatag caaaagctga
     2641 tgacactcca tttaaatcag aaattgatta aaattgtaag aatatagtat tttcaatagt
     2701 ttaaacaaac aaaaatgtaa taaaaaaggt gatttgccac ccatttgcca ccataattat
     2761 aatctaatgg caaattgttt taatgtacaa actagataat agaatattgt tttaaatgct
     2821 atattatgct aaaatttaat tgtattttgt aaattaaaaa acttataatt tagttttcta
     2881 gggttccgca ttttataatg gtcaggtcca agagaaaacg cgcaattgtt gtgtacacgg
     2941 aaggataaaa gcctgggaga tatttaataa tatttctata ggctttttta tttttacata
     3001 ttttaaattt gaggaggttt tctaatgaaa aaatttgtta ttgaggatgc attttggaat
     3061 ctatttccta gtgcaaaaat tggagttatt atttgtaatg atatagataa ctctataaaa
     3121 gatgaagact ataagaaaat aattttgaag ggagaagaag aagcattaaa atacttggag
     3181 gactcagaat ttagcaacaa ccaagtgatt aaagtatgga gagaggcatt taaaaaattt
     3241 aaaacgaaaa aaggagcaag gtcttctatt gaagcattat taaaaagagt tcataatgga
     3301 aataatatag gaactattaa ccctttagtg gatatctaca attttatctc attaaaatat
     3361 gcattacctt gtggagggga ggatatagat aaatttatag gagatattag gctaactaga
     3421 gcaattggta atgaggagtt tatcccacta gggacagatg aaaattcatt tccatatgaa
     3481 ggagaaataa tttataaaga tgatggtgga gcaatttgta gatgttggaa ttggcgtgaa
     3541 gcagtgagaa caatgcttac tgaagatact aacaatgcct ttttatgcat tgaattagta
     3601 gatgaaagta gatttaaaga atttgaaaat gctttaaatg aattagctaa aatagttcaa
     3661 gaaaaattgg gtgggaattg taaaatttca attttagata ttgataataa agaggtatca
     3721 gtggattaat ctggagatgc tgttgtaatg gactattcta gttataaaga tacttatatt
     3781 tagggtaata agttctattt tagatgtaac gattatcaaa ggatataata tagaaaataa
     3841 aaatgtagaa tataaaaa
//
LOCUS       DAEPLF010000272         3849 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEPLF010000272 DAEPLF010000000
VERSION     DAEPLF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.273"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            58..1170
                     /locus_tag="KR314_003347"
     CDS             58..1170
                     /locus_tag="KR314_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0329704.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1378..1854)
                     /locus_tag="KR314_003348"
     CDS             complement(1378..1854)
                     /locus_tag="KR314_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329705.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQPNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2053..3678)
                     /locus_tag="KR314_003349"
     CDS             complement(2053..3678)
                     /locus_tag="KR314_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0329706.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         1346 a          612 c          425 g         1466 t
ORIGIN      
        1 ttttgtgata tatatatgtt ataataaatg tataaaattt tgattggtgg gttatatatg
       61 aatgcattta ttagaaaaag aaataaaaac tatgttgttt acctggaatt tgaagatgaa
      121 aagacaggta aaagaaaaca aaaaaatatg ggtacttttg ataaaaaaag ggaagcaagt
      181 aaaagactaa atgaggtaaa agaaagctta tataatgatg gttttttagt accaagcgaa
      241 attacagtgt cagaatttct attagatttt ctaaataaat atagtgaaaa tatatcttta
      301 gctacatata ataattatgt tagtatttgt aaaaattaca ttaatccatc tattggaaaa
      361 tataaaatac aagacttaca tccggttcac atacaaaact acatagataa actatcttat
      421 aaactaaatc ctcaatcaat taagattcat ataaatatat tgaaacttgc aataaaaaga
      481 gcttatagac tcaaattaat aaaagaaaat gttatggata gtatagaggc tccaagatat
      541 aaaaaattta aaaatgagat ttatgataga gaacaaatga taaaattatt agatttagca
      601 aaaaatacag aaatggaatt acctattaat ttggctattg gattaggtct tagaatttca
      661 gaagttttag gtctaacttg ggataatatt gattttgaag aaaatacaat aacagtaaat
      721 aagataactt cccgaattaa tggttctgtt attctcaaag agccaaagac agaaagctct
      781 gttagaaaaa tatcttctcc caaagaatta atgtctttat taaaagaata taaaataaaa
      841 cagaataaaa atttattaaa atcttccatt agaaataata ataatttgtt gtttttcaat
      901 aagaaatgtg aacctattgc tgaagatgtt atgagtaaaa aatttaaaag atttttagaa
      961 aagaatgaat tacctcatat tagattccat gatttaagac actcacatgt tactttactt
     1021 ataaattcta aagtacctat aaaggttata tccgagagag taggacactc aaatataagt
     1081 actactctaa gcgtttattc tcatgtactt aaagaaatgg ataaagaggc ttctgacaaa
     1141 atttctgaaa gtttatttaa tgctaattaa tattaattta tcttagcttt aaattagctt
     1201 agcttattga gatagattca ttaatattaa atgtatctat ctcaaattaa taactactta
     1261 aataatctgt tttattttaa tttatcgcct cagttattat acttttttat gaaaaattat
     1321 actttacaat aaaaaagata tttccttata gaaagtatct ttttaaatac tattttatta
     1381 tatacttgct aacattggaa tatctaacat cttcttcatt acatcatcta ctttttttgc
     1441 tttatattca ttgtatccaa ttaaatatcc tttttcatat gaacttttta aaatctcttt
     1501 ttcatataaa ttatcagatt tggcttctgt gacacttaaa ttataaagcc ctttacaccc
     1561 atcttcataa cctttattat accctttttc tgcaaaatag tttttatgat ttttttcgtt
     1621 atgtactgaa gatattgtta ctccagaatt ttcttttgta ctagcataag gacaaaaacc
     1681 tttatttggt tgatgtgctg aatgtcctcc acaataataa cgtgttaact ctgatttatt
     1741 tttattatga tgacctcctg atgaattatg ccctcctgag tgagcatatg aaaaattccc
     1801 tacactaatc atagctaaac tcaatactac agctactaac cttttcttaa ccataatcta
     1861 ttccccccaa aatgttatat atttttatta tatcatctac tatattatat ttaaattctt
     1921 aatatacaaa atattactcg attctgctaa taaacatatt taaacaacat aaaaaacctc
     1981 ctaatatttg attaaaaatt tacacactca tgcatagcat gtatcaaata ttaaaaggtt
     2041 ttatactaat aattatttat cttttccata cattttagct aattggtctt gtatctcttt
     2101 actttctaaa tactcatcaa agtccatttt tctatccatt attccatttg gagttatctc
     2161 tataattcta tttgctatag tagatataaa ttcatggtca tgagaagtaa ataaaagcac
     2221 tcctgggaac ttttcaagtc ctttatttac agaagttata ctttctaggt ctaagtggtt
     2281 agtagggtca tctaatacaa gtacatttgc atttgaaagc attagcttac taagcataca
     2341 tctaaccttt tctcctccag atataacttg agcttctttt aatgcttctt cacctgagaa
     2401 taacattcta cctaaaaatc ctcttataaa actttcactt ttttcttcag agaactgtct
     2461 aagccagtca accaaagaat attctactcc gtcaaaaaac ttattatgat tttttggaag
     2521 ataatcttga gaagtagtta ctccccattt atactctcca ctgtctggtt caagttctcc
     2581 cataactatg ttgaaaagtg cagtagtagc tatttcatct cccataaata ctactttatc
     2641 atctctatcc aatctaaatg acacattatc aagaacctta accccatcta tagttttagt
     2701 taaattgtgt acttctaaaa cttcattccc aatttctctt gcaggtgtaa atcctacata
     2761 tggatatctt cttcttgatg gttgtatatc ttcaacttct aatttctcta actgtttctt
     2821 tcttgatgtt gcttgtttag cctttgaagc attagaacta aatctagcta taaattcctt
     2881 taattgagct atcttttctt cagtcttttt attttggtcc ttagccagtt gtaaagccaa
     2941 ttgacttgac tcataccaga aatcatagtt tccaacgtac atttgtattt taccaaaatc
     3001 tacgtcaact atattagtac atatttgatt taggaaatgt ctatcatgtg atactattat
     3061 aactatagac tcttctaaat ccattataaa attatttaac cagtttattg atttaaaatc
     3121 taagtggttt gtaggttcgt ccattaatag tatttcaggc ttaccaaaaa gtgattgtgc
     3181 taacaatacc ttaacctttt ccccaccaac tagttccttc atttgtttgt aatgcatatc
     3241 tttcgttatg ccaagaccca taagtatttt ctcagcatta gtttctgcat cccatccgtc
     3301 aagctctgca aactcacctt caagttcggc agcttttatc ccatcttctt cattaaagtc
     3361 ttccttcata tacaatgcat ctttttcttt cattatgttc caaagtctct catgacctct
     3421 tataacaaca tttaaaacag tttcttcttc atattcaaag tggtcctgtt ttaaaactga
     3481 cattctttct ttatcagtta tagatatact acctgtatta ggctctattt ctcctgataa
     3541 tatttttaaa aaagtagatt ttcccgcacc attagctcct attattccat agcaattacc
     3601 tttagtgaat tttaagttaa catctttaaa tagttcctta tcaccaaatc taagtccaac
     3661 acctgtaact tgtaacatta atatattttc ctttcttttt gctcataatt tattttatca
     3721 taaaactttg ataacatttt tattatatat tatcacgtaa atagtatatt ttttatacta
     3781 aatttgtgaa atatttatta aaataatact aaaaattgta aatttataaa aattaacaat
     3841 ctcttatat
//
LOCUS       DAEPLF010000273         3844 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEPLF010000273 DAEPLF010000000
VERSION     DAEPLF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.274"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(174..743)
                     /locus_tag="KR314_003350"
     CDS             complement(174..743)
                     /locus_tag="KR314_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBH0329707.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            complement(815..1423)
                     /locus_tag="KR314_003351"
     CDS             complement(815..1423)
                     /locus_tag="KR314_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0329708.1"
                     /translation="MDSNDKKGLKSTEIIIINGSPRINKNCSFIANKVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRNKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            complement(1431..3194)
                     /locus_tag="KR314_003352"
     CDS             complement(1431..3194)
                     /locus_tag="KR314_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBH0329709.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDTIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMKDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(3237..3704)
                     /locus_tag="KR314_003353"
     CDS             complement(3237..3704)
                     /locus_tag="KR314_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329710.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMIAFNLNMILGIGVFLIGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
BASE COUNT         1262 a          672 c          346 g         1564 t
ORIGIN      
        1 atataatttt accatattac atcatatttt tgtaagtttt tattacattt tttataatac
       61 ccctttattt tataaattaa tctaatcgat taaatttatc ctatagaaat ttattacatt
      121 taaaaatatg ctagcaaata aaatatctac tagcataaag tctatttata atattaatca
      181 actattaatt ctactaaatc tccattttta acgcctgctg catttccctc ttccatatca
      241 acatgcattt ctagattaaa gctatcacta gctcttacta atacattttc aaatactaat
      301 gctcttggtc cagatgtttt aacttttact atttgcttat cagttacacc aaatttagca
      361 gcatcttcat tgctcatatg tatatgtcta gaagcaacta taactccttc tttcatttca
      421 acttctccag caggtcctac taatttaaca cctggagttc cagctatatc accagattct
      481 tttattggtg cttttactcc taatgggaaa ccatctgcta aagatatttc aacttgagta
      541 tgagctctag ctggtcctag tactctaact ccctttaaag ttccttttgg tcctactaaa
      601 tccacttttt cttcacaggc atattgccct ggttgagata atggtttaaa tttagttaac
      661 tcatgacccg ccccaaataa tacgtctata tcagcttgac tcaaatgaac atgtttatta
      721 gataatgcta ttggtaattt cattgaaatt ccctcctttt aattatttaa attattgcaa
      781 tacaattata cataatatta cctacaaaat caaattatat tgaatcaata tatatcttta
      841 aatttttata tattatatct ttaaattctt gattatctat ataagataca tcatctgtat
      901 tgtttaaata tatattgtgt aaaagtttag tatttatact cttaaagaaa aaatccataa
      961 caatctgtcc acctttaaac tgtgaatcat atgatttact cccccccact gcaatataca
     1021 ttcctatccg tttcttattt ctatcaattg aaggtttatt taatacatat ttacttgcat
     1081 atatagcttg agttctatca acaacagtct ttaatttagc tgatattgaa tcaaaatgta
     1141 caggacttat tactatagtt ccttttgaat catcaaacat tttatataat ggcttcatat
     1201 catcattaaa cttgcaatac cctgtttttt cacaaaatcc acatgctgtg caatattcaa
     1261 tatccatatt gtagatatta aatattttac aacttaaccc acactcttct agcatagctt
     1321 ttactttatt tgctataaaa ctgcaatttt tattaattct tggacttcca tttattatta
     1381 taatctctgt acttttaagt ccttttttat cgtttgaatc cataatcaat ctacctatcc
     1441 tttaacataa ctattaaaat atcatcatca taattatctt ttgatgagaa attctttaaa
     1501 tccaatttta gattttctac tattaactgt tgatttaatc taaaattttc ttttagaaag
     1561 ttttctaatc tgtttatacc atactcttct tttgatgaat tttttatctc taaaattcca
     1621 tcagtgtaca tacataccat tgcataatct tccattgaaa gagtattatt ttcataagta
     1681 gcatctttca ttattccaat aggtatgccc tttttacaat ttaaattatt ttctattgtg
     1741 ccatctttct ttacaactac aggactatag tgacctgcat tagacacagt aatcatattt
     1801 tctttagtat ctataatacc tatcaaacaa gttgtaaata ctcccatctt gtcaaattca
     1861 tcatataaca tcctatttaa atttgtcatt atttctcttg gtgtattgta ttgattgcat
     1921 aatactttaa aagcaccttt tatcattgca acaacataat ttgataccat tccatgtccc
     1981 attacatcag caacaatata aactatatgt tcgtcatcca cttttgtagc ataataaaaa
     2041 tctccaccta ctacccttgc tggtttatgg taaaaataaa gttctttctc atcattacaa
     2101 tccattttac ctttatccat aataagctgt tgttgtttat ttaatatgtt caactcattt
     2161 tcaagaactt tgtgttttac ttccttttca ttatgttcat ataattgcat cccaacagaa
     2221 acttgtttgg acaatatact aagaatattt aaatcatcac tagtatagtt tttactattt
     2281 gcacaaacta tacatcctat agtctcattt ttatcctgaa gtttgtaata tatataactt
     2341 tccaaattta tgcccttttt tgagcatggt tttaaattat ttaggtatcc ccctaatgac
     2401 tctttcttta aaaacatatc aatcatagga tataaatttg ttttcttttt ttgctcaaca
     2461 ttataacaac taactatagt atccttatta tgatgactat ttataattat taaagcttct
     2521 ttactattag taatattaca cgcttgttta cttaaatttt ccaaaaagcc atcaatactc
     2581 ttattaaggt atagtttttt agtagcttca tttatggcac ttattgagcc tcttagttta
     2641 tcaattaaaa tttgtacatt attattttta ttagttattt ctattgataa acttaagagt
     2701 gatgcaacag atgatataaa atctatatct tgttctgtta tataatcatc ctcttttaaa
     2761 aaacaagtca taaatccaac aactacatta tttattataa aaggaaaaac tattctccca
     2821 atataacctt cagcttttgc taaatctcgt tcaccttctg ctctagaatc ttcaaataca
     2881 ttttccacat atattatctt tttttcattt acagcctcat gtatatactc tggatatgtg
     2941 tcaaaatcaa tttttgcacc tttaagacta tttattggaa aaatttgagg tacatattct
     3001 aaggtttgcg aacatactaa gtaggcatat ttagaatcat ttttataaaa tagatttaca
     3061 catgcctttg tagggtgaac aacttctaac attttttcta ttatgtcatc ttttatatta
     3121 aaaaaatctg tagattccgt caccatagcg gatatctcca ctaaattctt cattttataa
     3181 ctgccattag tcatactcaa tttctccccc tatatattaa actatttatt agtattttaa
     3241 catacattct ttatagtaaa aacaaaaaca acgacaaaac tagcaactaa tatccagtta
     3301 aatcctgtca ataatcctat aagaagtaat gtaatcaatg catttagacc tgttctccct
     3361 ttttcatcca taaaagttat tttagagatg tcgatattat ttttcacatt atttatatgc
     3421 tcttttactt gttcttccat atttctttta tatagtatat aaactacacc aattaaaaac
     3481 actcctatac ctagaatcat gtttaaatta aaagctatca taagcagtat tgaaccaaaa
     3541 gccaacttta aaagcttaaa aaatacgcta tctatcttta gagttatatt tctgatttct
     3601 ttacattcat ctcttaaaga atatccactt gagtctaggt tattaatctt tgtttttaca
     3661 atttcagaat aaaaaagctt tattatccca tttaacattt tcatcactcc taaatatttt
     3721 acatatattt gtaagtttta caattataat attttttata cttggtaata ataaaaaata
     3781 acaaatattt tatgatttag tatttactgt aaataaatta ttgaagcttt attagttaag
     3841 tata
//
LOCUS       DAEPLF010000274         3819 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEPLF010000274 DAEPLF010000000
VERSION     DAEPLF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.275"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            85..1038
                     /locus_tag="KR314_003354"
     CDS             85..1038
                     /locus_tag="KR314_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBH0329711.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAVFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            1028..1984
                     /locus_tag="KR314_003355"
     CDS             1028..1984
                     /locus_tag="KR314_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBH0329712.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            1981..2736
                     /locus_tag="KR314_003356"
     CDS             1981..2736
                     /locus_tag="KR314_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0329713.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            2828..3793
                     /locus_tag="KR314_003357"
     CDS             2828..3793
                     /locus_tag="KR314_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0329714.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKTSLPASLEKYKDKKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         1534 a          360 c          655 g         1270 t
ORIGIN      
        1 atgataatta ttattgataa actataaaag tgaaaatgat aataattttc attttaatta
       61 ataaaagatt tttaaggagg gagaatgaag aaaaggtatt tagtcatagg aataataatt
      121 ttgtcttatt tttctttatt tataggagct gaagatataa atataatgca tatatttgca
      181 aaaaatcagc ataagcttat gatatttatc atgagtaggg taccaagatt aataagtata
      241 ttgattgctg gtgtaggaat gagcatagca ggtcttataa tgcaacagat aagtaagaat
      301 aaatttgtat cacctacaac aggtgctaca atagatgcag ctcaatttgg gatagtaata
      361 tgtatgcttt tagtaccaac tgcctctatt tttacaaaga caataatagc atttgtattt
      421 tctcttgtag ggacttttac gtttatgaaa ataataggga agttacaatt taaaaatata
      481 atctttgtac cattagtagg tataatgttt ggaaatataa taggttctat gacggatttt
      541 atcgcgtata aatacgattt gagtcaaaat gttagttctt ggatgcaagg tgacttttct
      601 atgatactta aaggaaatta tgagatatta tatatcacaa taccccttat aatattagca
      661 tatatttatg caaataaatt tactgttgtt ggtatgggta tggattttgc gacaaactta
      721 ggtcttagtt acaaaagaat tgtcaatata ggtctgataa tagtagcact tgttacagta
      781 tgtgtagtcg ttacagctgg taatatccct tttataggtc taatagttcc caatatcgta
      841 tccttgtata tgggtgataa tataagagca agcatatggt atactggttt acttggagcg
      901 gtatttgtac ttatatgtga tatttttgga agaattatta tttatccata tgaaatatct
      961 ataggactta cagttggagt tataggaagt atactattcc tttatttaat attaaggagg
     1021 aatgtgaatg aagcttagtc tcaatcaaaa aatttacttt gtaagtatat tactattcat
     1081 atcaatagca ctttttataa tttatggaat agacatgaat catttagaat atgcattatc
     1141 acaaagaata ccaaaaatat ttgctatggt attaggtgga ggatgtatag catttactac
     1201 tgtagtgttt caaacaatta caaacaatca aatattaaca ccaagtgtac taggtttaga
     1261 ttcattgtat gtaatgattc aaactataat agtattcata tttggttcaa gcagtagtct
     1321 gattataaat gaaaattata attttattat taatgtagct atgatgattg gagcttcatt
     1381 gttattatat aagatgttat ttgaaaaaaa taaaaataat attttctttt taatcctagt
     1441 aggtatgata tttggaacct tatttaaaag tgcaacaaca tttattcaag taatgataga
     1501 cccgaatgaa tttttagctt tacaaactag tataatggca agtttaaaca atataaacac
     1561 taatgtattg ttaatagcat ttataataat tattgccata atacctttta tatatgatga
     1621 aataaagtat ttagatgtat tgtcgttagg taaagaacaa gcaattaatt taggtgtaga
     1681 ttttgataag gcagttaaaa aaatgataat attaatagca attttagtct caatatcaac
     1741 agcacttatt ggacctatga catttttagg acttttactt gctaatataa caagagaaat
     1801 ttttaaaaca tataagcata catacttgat ttcgggttct atgttgatag ggatgattac
     1861 attgataata ggtcaatttt ttatacaaca tgtatttaag tttgatacga ctttaagtgt
     1921 agtaataaat tttataggag gaatttactt tattcaatta ctattaaagg gggcaaatag
     1981 atgatagaga taaaaaatat atttaaaaga tataagaata aaaatgttgt agatgatgtc
     2041 tcttttagta tagaaaaggg aaagataaca tcttttatag gaccaaatgg agctggaaaa
     2101 agtacagtac tatcaatagt tacaaggctt ataggaggtg atggcggaga agtaataata
     2161 gaaggaaaaa gcttgactaa ttatagcaat aaagaattag caaaaaaaat agctattctt
     2221 aaacaatcta acaatataac tttaaagctt acaataagag aattagttgg atttggaaga
     2281 tttccatata gtgaaggtaa tctaactaaa gaggatgaaa attatattga tgaagcaata
     2341 gaatatatga agttgactga tatacaacat aagtatttag atgaattgag tggaggtcaa
     2401 agacaaagag cttatatagc aatggtaata gctcaagata cagagtacat attacttgat
     2461 gaaccactga acaatttgga tatgaaccat tccgtacaga tgatgaaggt acttagaagt
     2521 ctttgtgatg agttagataa gactatagta ttagtaatgc atgatataaa ttttgcatca
     2581 tgttattcag ataatatagt tgcattaaaa aatggaaaag ttgaaaaagt aggtcgtaca
     2641 gatgagatag taaatgaaaa agttttagaa gatatttatg agatgaattt taacattaag
     2701 aatataaatg gaaatcgaat ctgtatttat ttttaaaata ttgtatatga aaataagctt
     2761 gctatatgat tttttataaa gtttttaaat taaaataaag attaatagga gagaggaaaa
     2821 tattgatatg aataaaaaag cagcaatagt agcagcagta gctattatag gattagtgac
     2881 agtgtttgca ttaggtggaa gtaaaaagaa tgaatcaaaa acatcagaaa attcaaataa
     2941 taccataaag ataactcata acttgggaga aacagatgta aaattaaatc ctaaaaaagt
     3001 agtagtattt gattattcgg ctttagatac aatggatgca ttaggtgtag ctgagaattt
     3061 agtaggactt ccaaaaacga gtttaccagc atctttagaa aaatataagg ataagaagta
     3121 tgcagattta ggaggtttaa aagaaccaga tttagaaggt ataaagtcgg caaatccaga
     3181 tttaattata ataaatggaa gacaagaaga tttttatgaa caattgtcaa aaatagcacc
     3241 aacaataagt acaagtaaag atgataagaa gtacttagaa tctgttaaaa ataatataga
     3301 caaaatagca aaaatatttg gagtagaaga aaaagcaaat caagaattta gtaaaattga
     3361 aaagaaaata gaaactttga ataaaaaagt aacagataaa aatttaaatg ctttaactat
     3421 aatggtaaat gaaggtaatt taagtgtatt tggtgaagaa tcaagattta gtatattata
     3481 caatagtttt ggatttgaaa ataaagataa aaatataaaa gaatcaagtc atggacaaaa
     3541 tattactttt gaatatatag ctaaacaaaa tccagaagtt atgttcgtta tagatagagg
     3601 aatagctact ggtagtgatg taaaagaaag ctcaactgca aaatctgttt tgaacaatga
     3661 tataataaaa tctatggatg catataagaa tgataatata atatatttag attcaccaac
     3721 atggtatgta aatgatggtg gtctaacatc tttaaataaa atgatagatg atgcttcaaa
     3781 agcggtaaat taataatccg atataatcaa aatgtattt
//
LOCUS       DAEPLF010000275         3803 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEPLF010000275 DAEPLF010000000
VERSION     DAEPLF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.276"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..595
                     /locus_tag="KR314_003358"
     CDS             <1..595
                     /locus_tag="KR314_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBH0329715.1"
                     /translation="IIAVIFIISVTMLILISHINILIIADINNKDICLKLNIKYMFNL
                     ININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHELYSNINFG
                     TGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHIRPRIKAL
                     FNIIIMINKIMNKNKGNKEGDSNESNRFDTKSYGNNS"
     gene            549..977
                     /gene="ytfJ"
                     /locus_tag="KR314_003359"
     CDS             549..977
                     /gene="ytfJ"
                     /locus_tag="KR314_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBH0329716.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1225..1665
                     /locus_tag="KR314_003360"
     CDS             1225..1665
                     /locus_tag="KR314_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0329717.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1681..3132
                     /locus_tag="KR314_003361"
     CDS             1681..3132
                     /locus_tag="KR314_003361"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBH0329718.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAIETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3223..>3803)
                     /locus_tag="KR314_003362"
     CDS             complement(3223..>3803)
                     /locus_tag="KR314_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBH0329719.1"
                     /translation="NILPYSYTVEKIICTLLVIIMALEQLLLISSGNYSTLNSLPIGI
                     NYICIYLCIAILIFKQYHLFNIFFSWSLVCSVGELIFSKNLGYEFPSLIYFIFIFSKC
                     LIIYADIYMVDVRKFRVNRYALRDNLAICFIYFSFIFLLNTFTNSQYYYGFLSHSTTA
                     IFTFIFVTSIMYIPALLFNRDTFILEKKKKSK"
BASE COUNT         1585 a          349 c          654 g         1215 t
ORIGIN      
        1 aataatagct gtaattttta ttataagtgt aactatgttg attttaatat ctcatataaa
       61 catcttaata atagctgata taaacaacaa agatatttgt ttaaagctta atataaaata
      121 catgtttaat ttaataaata taaatagaca actttatcca gctgaaaata gtaaaaataa
      181 tgataaaaaa gaggggatga agaataatat agattcaagt atattattag ctgatgacct
      241 tttgagcatt tatagattat taaaaaagat aaaaatacat gaactttatt caaatattaa
      301 ttttggaact ggaaatattg gcttaacatc ttctgtatat gtattgataa atactttgta
      361 tggaaattta tttaatatga ttgatgcaga aaaaatgtat ttaaatgtaa atccagattt
      421 tacaaaagat tatgttttag gaaatataag gatacatata aggccaagaa taaaagcttt
      481 atttaatatt ataataatga ttaataaaat tatgaataaa aataaaggaa ataaggaagg
      541 tgatagtaat gagagcaaca ggttcgatac aaagtcttat ggaaacaact cttgaaacaa
      601 ttaaaggttc tatagatgca aatactatta taggagaccc tataaaaaca gatactacag
      661 tggttgtacc tatatcaaag gttacaattg gctttggtat aggaggagga gaatactcaa
      721 aaggatatga tgataaagat agggaaattg aattaaaaaa tgaaaaatca gatacgaatt
      781 ttgctggagg tagcgcaggg gctatatctg tacaaccagt agcatttgta gtagttgaaa
      841 gtggagagac tagaataatg agtttagaca gtaatatcaa tctagtggat aatatattat
      901 ctataactcc tagagtattg gagaaaattc aaaatatatc acaaagtaat aagaatcaag
      961 ataaaaacaa tatgtaagaa taaaatattt ttaaatatgt atatagtttt aagtaaagta
     1021 gtggggttag tataaatccc actattttgt tgtgtacttt aaacatttat atctaataag
     1081 attttagttt aaaacaattt aaatagataa taaagttgat atatagtata attataaaaa
     1141 gaaaagaagt attatattat agatatataa acgcatttga gatataagag ggagttataa
     1201 atcattagtt ttgaggtgaa tgttatgagc gataaagaat tatctatttt gttgttagaa
     1261 agtatgaata attttcataa tttaataaag attattaata acgaaagata taaaaaagat
     1321 aaggttctta cagaaagaca gttctttgcc ttagtaaaaa taagaaaaca tgataaaata
     1381 gagcttaaaa atctaagtag agatttacat gtatctactt ctagtctgtg tatactactt
     1441 aataaattgg ttgagcaaga gtatgtatat agggaagaag atagtagaga tagacgaaat
     1501 actttttatg gaataactaa aaatggagaa aagatattgg ataatgaaat actaaagttt
     1561 gtatctataa taagtgataa aatggattgc ttagatatag ataataaaga taaattattt
     1621 acttctttgg aagagtctaa aaatataata gaacaattat tttaaaaagg agaatttatt
     1681 atgaatataa agtattctaa agagatatta aaaagtttag aaaatgctat aacagtagta
     1741 gctctatttg agggagaaga tgtttctacg acatttgata aaataaatat gataagcaga
     1801 aattttaatt ctaaagaaaa tagtatagat actgtaacat tggttgaaaa taacaacttg
     1861 gtaaatgttt gtcttgttgg ttttggtaag agagagttat tgaatagaga aaaaattaga
     1921 atcataggtg gtaatttgtg taaaaaatta aaaaatatat tgaaaaatga agcatataag
     1981 agtagtgatg ttaatgtact taatcttaat ttaaatagtg atgaaatagg ttcttttgta
     2041 gaagggatat tacttggaaa ttataagttt gataagtata agacaaaaag taaggatttt
     2101 gaaagaaaag aagttgatac tataactata tttactgaaa aagaagttga gtgtcaaata
     2161 gaaaaggcta aaatattagc taattcaact ataatagcta gaaatctagt taatgaacca
     2221 tcaaacgtaa tatatccaaa aacattagca atagaaacag ttaaacttgg tgcagagttc
     2281 gggtttaatg ttgatgttta tgaagaagaa aaaataaagg cattgggaat ggatgctttt
     2341 ttcgccgttt caagaggttc aatcaataaa ccaagattca tagtaatgag atactttgga
     2401 gataaagaaa gtacagatat attaggtcta gtaggtaaag gattgactta tgatactggt
     2461 ggatattctt taaaatcaaa tgcttctatg ctagatatga agacagatat ggctggtgct
     2521 gcgagtgtta taggtgctat gtgtgctata tctcaaagta aattaaagaa aaatgttata
     2581 gcagtagtgg ctgcatgtga gaatgcttta tcaggaggtt catacaaacc tggagatata
     2641 attagttcta tggcaaagaa aactattgaa gttttaaata cggatgccga aggtagactt
     2701 acattagctg atgcaatata ctacattata aacaatgaaa aagtaacaaa ggtagttgat
     2761 gtagctacat taactggtgc tgctttaaca ttgttaggaa atgttgcaac tcctatagta
     2821 acaaataatg atgattttta ttgtgaatta gaaaaagctg ccactttatc aggagaaagg
     2881 gtatggaaga tgccaattta tgatgaattt aaagatatga taaaaggaga agaagcagac
     2941 cttaaaaaca ctggtggtaa aaatgctgga tgtataacag caggagcttt tattggtgaa
     3001 tttgttggga atactccttg gatacatatg gatatagcgg ggacaagtac atcatcaaaa
     3061 tcaattggat acaaagcaaa gggggctaca ggagaacctg ttagaacatt atactatttg
     3121 gcagaaatct aaaaaacaat tataaaatat aatttataaa gccacatgga tatttttgta
     3181 atttaagatt atctatgtgg ctttattttt tatttaagta aattatttac tctttttctt
     3241 tttctcaagt ataaaagtat ctctattaaa taaaagagca ggtatgtaca taatactagt
     3301 aacaaaaata aacgtaaaaa tagcagttgt gctatgactt aaaaaaccat aatagtattg
     3361 tgagttggta aatgtgttta gtaaaaatat gaatgaaaaa taaataaaac atatagctaa
     3421 gttatctcta agtgcatatc tgttaactct aaattttcta acatcaacca tatatatatc
     3481 tgcataaatt atcaagcact tagagaagat aaatataaaa tatattaagc taggaaattc
     3541 atagcccaag tttttagaaa aaatgagttc tccaactgaa caaactaaac tccaagaaaa
     3601 aaatatatta aaaagatgat actgcttaaa aattagtatg gctatacaca gatatataca
     3661 gatataattg attccaattg gtaaagagtt tagagtacta taattacctg aagaaataag
     3721 taataattgc tctagagcca taattattac aagtaatgta caaattatct tctcaactgt
     3781 atagctataa ggtaaaatat ttt
//
LOCUS       DAEPLF010000276         3765 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEPLF010000276 DAEPLF010000000
VERSION     DAEPLF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.277"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            6..623
                     /locus_tag="KR314_003363"
     CDS             6..623
                     /locus_tag="KR314_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH0329720.1"
                     /translation="MKSFNVTVILAISSLVFSLIIGTIVALSSYYKVKVLNQICKVYV
                     SIFRGTPLMPQLFFLYFGLAYMNDFVKNMDPVFATSIVLSLNMGAYMSETIRASILSI
                     DKGQIEAAYVMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLIKGSSVAFVVGVNDIM
                     GAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKISNY"
     gene            683..1480
                     /locus_tag="KR314_003364"
     CDS             683..1480
                     /locus_tag="KR314_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0329721.1"
                     /translation="MVKRNIIKCLVAVLSLTFVVAGCSKKEEVKDEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVAASVNKD
                     NLFEEKVYGDGTNGLQDLDLGRVSAWIDAELTVVGDAKKANMKVRVLDEKLSVTNIAY
                     FFKKNDEKSKTIKEDVNKAIEEMLSDGTVKKISEKWFGMDVSADIQK"
     gene            1613..2788
                     /locus_tag="KR314_003365"
     CDS             1613..2788
                     /locus_tag="KR314_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0329722.1"
                     /translation="MIETKIKSIALKNREKIIKIRRQIHSNPELAFKEFKTSKLIKEE
                     LNKLKIEYIDVAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSINDNMHA
                     CGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGVDALAT
                     GHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAVYSSIQ
                     QIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKILENIV
                     RNVCRAYGADCKFEKSTGGDAVINDKNMIELGRESACKILGVENVEIIDFPAMTGEDF
                     AIYMKEKPGLFMYIGVGNKEKNINYRLHSNKFNIDEKCLSIASSLFSQLAVDYLCQL"
     gene            2897..3646
                     /locus_tag="KR314_003366"
     CDS             2897..3646
                     /locus_tag="KR314_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBH0329723.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEINQSEVVAILGPSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDIANLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSNKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIVRKEEVFS"
BASE COUNT         1494 a          379 c          724 g         1168 t
ORIGIN      
        1 taataatgaa atcgttcaat gtaactgtaa ttttggcaat aagttcatta gtattttcat
       61 taattatagg tacaatagta gcattatcat cttattacaa agtgaaagta ttgaatcaaa
      121 tatgtaaagt atatgtatct atctttagag gtacgcctct tatgccacag ttattctttt
      181 tatattttgg actagcgtat atgaacgatt ttgttaaaaa tatggaccca gtatttgcaa
      241 catctatagt acttagttta aatatgggag cttatatgtc cgaaacgata agggcatcta
      301 tactttcaat tgataaaggt cagatagaag cagcatatgt aatgggatta acaaatttac
      361 aaacaatgaa aaggatagtg ataccacagg ccgtaagagt tgcactgcca tcattattca
      421 ataattttat agatttaatt aaagggtctt ctgtagcttt tgttgttgga gtaaatgata
      481 taatgggtgc tgccaaatca caaggagcgt tgagttttaa gttctttgaa gtgtatggag
      541 cagttatggt aatttactgg tgcataataa ctcctttagg gtgggggcaa aaaaaattag
      601 aaaagaaaat aagcaattat taagttatta agaaaataca taaaagtatt tttatataaa
      661 aaatggtgtg aggagaagag atatggtaaa aagaaatatt ataaaatgtt tagtggcagt
      721 tttatcgtta acatttgtag ttgctggttg ttcaaaaaaa gaggaagtaa aggatgaaaa
      781 agttataact gtagcttcat ctaattcagc tgacccatat tcttatgttg ataatggtga
      841 gcataagggt tttgaagttg atatgtggaa agagattggt aaaagaagtg gatataagat
      901 aaaaatggaa ccaacaggtt ttagttctat atttgggatg ttagattctg gtaaagttga
      961 tgtggcagcc aatttctttg gaatgtctaa agaaagatta gaaaaatacg atgcttctat
     1021 accttatggt tctgattctg tatgtatagc tgtaaaagat ggaaacaacg aaattaataa
     1081 acttgaagat ttaaaaggaa aagttgtagc agtttcagaa ggttctcaag ggcaacaggt
     1141 tgcagctagt gttaataaag ataatttgtt tgaagaaaag gtttatggtg atggaactaa
     1201 tggtcttcaa gatttagatt taggaagagt tagcgcatgg attgatgcag aacttactgt
     1261 tgtaggagac gctaaaaaag ctaatatgaa ggtaagagta ttagatgaaa aattatcagt
     1321 tactaatata gcatacttct ttaagaaaaa tgatgaaaaa tctaaaacta taaaagaaga
     1381 tgtaaataaa gcaatagaag aaatgctttc agatggaaca gtgaaaaaaa tatctgaaaa
     1441 atggtttgga atggatgtaa gtgctgatat acaaaaataa atactttaaa attaaaatat
     1501 aaaactgtga taactataga taagtatact aaatttatct atagttatct gtgtattaca
     1561 atgtatttag attagtcttt ataaattaaa atgagttatg gaggtcttgc aaatgattga
     1621 aactaaaata aaatccattg cactaaaaaa tagagaaaaa atcataaaaa taagacgaca
     1681 gatacacagt aatccagagt tagcatttaa agaatttaaa acatccaaac ttataaaaga
     1741 agagttaaat aaacttaaaa tagaatacat agatgttgct ggtacaggag tattagctac
     1801 tataaaagga aaaaataatg gtgggaaaac tatattgctt agagcggata tggatgccct
     1861 tccaatcaaa gaggaaaatg atttagaatt taaatcaata aatgataata tgcatgcttg
     1921 tgggcatgat gcccatgttt catggttgtt aggaacagca atgattttaa atgacataaa
     1981 agaagaattg aatggaaatg taaaattact atttcaacct ggcgaagaaa aaggtggttc
     2041 tgatattgta attaaagaaa atgtacttga aggtgtggat gcattagcaa ctggtcattg
     2101 ttggccaact attgaatcag gaaaaattgg aatcgctaga aattgtgcta tggcagcgac
     2161 taatactttt gaaataacaa taattggcaa gggcgggcat ggagcagagc ctcataattg
     2221 tatagaccca attgcagttg gaaatgcagt atattcatcc attcaacaga tagtttcaag
     2281 aaaaattgac ccagttgtac ctgtagtagt gtctgtttgt tcttttaatt ctggtgtttc
     2341 taaaaatata ataccagatg tatgcacact tcaagggact attagagcga taagcaagga
     2401 acaagtaatt gaagtaagta aaattctaga aaatatagta agaaatgtat gtagagcata
     2461 cggagcagat tgcaaatttg aaaagagtac aggaggagat gctgttataa atgacaaaaa
     2521 catgatagag ctaggaagag aaagtgcttg caaaatactt ggagttgaaa atgttgaaat
     2581 aattgatttt cctgcaatga caggtgagga ttttgctatt tatatgaaag aaaaacctgg
     2641 tttatttatg tatataggtg taggaaataa agaaaaaaat ataaactaca gattgcatag
     2701 taacaagttt aatatagatg aaaaatgtct tagtatagca tcttcattat tctcacaact
     2761 tgcagtagat tatttatgtc aactatagag tacattcata aaaatatatt tagcctattt
     2821 ttaaatttaa tgctagtata ttaattttga tgtagaacat attatttatt atatttattt
     2881 aaaaaggtgg ataacaatga taaaagtaga aaatttacat aaaagtttta aagggacaga
     2941 agttcttaaa ggtataaatt tagagataaa tcaaagtgaa gtagtagcta tacttggacc
     3001 atctggaaca ggtaaatcta cgttacttag atgtttaaat tatttagttg agcctacaaa
     3061 aggaaagata atgattggtg atgtaaaagt agatgtagaa aaagcaacaa aaaaagatat
     3121 agccaattta agaaaacata catctatggt atttcaaaat tataacttat ttaaaaataa
     3181 aacagcatta gaaaatgtaa tggaggcatt gatagtagta cataaaaaga gtaataaaga
     3241 agctagagaa atatctttaa aattaataga taaagtaggt atgttggata ggaaagattt
     3301 ttatccatca aaattgtctg gggggcagca acaaaggata ggtatagcaa gagcgatggc
     3361 agtaaatcca aatgtaatat tatttgatga gccaacatct gcacttgacc cagaacttgt
     3421 tggagaagtt ttaagcgtta taaaatcgct tgcgaaagaa ggaataacaa tgcttattgt
     3481 aactcacgaa ataaattttg caaaagaagt tgctgataaa attatattta tggataatgg
     3541 tgtaatagct gaagaaggaa ctcctgatga aatatttaat aatcctaaaa atgaaagaac
     3601 tgcaaaattt ttaaatatcg ttaggaaaga agaagtattt agttaaaagt gtataaatta
     3661 agtattagaa gatatgtaag atactcccat taaatttata agttagataa taaatttaat
     3721 ggaagtatct ttttattatt agatttatta tttaatttaa aatta
//
LOCUS       DAEPLF010000277         3693 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEPLF010000277 DAEPLF010000000
VERSION     DAEPLF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.278"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(34..1455)
                     /locus_tag="KR314_003367"
     CDS             complement(34..1455)
                     /locus_tag="KR314_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBH0329724.1"
                     /translation="MVSFLGSIVVLIVGYFIYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGIVVAI
                     ILGLLFFKKAKDIKEQNAHKLAS"
     gene            complement(1601..2371)
                     /locus_tag="KR314_003368"
     CDS             complement(1601..2371)
                     /locus_tag="KR314_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0329725.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSSIEVVNEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYENYAVDAFSLDVFDYVLKP
                     YSEERIISMLNKLEKSEMSDIELSNSNSNLYKYKKETVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(2376..3578)
                     /locus_tag="KR314_003369"
     CDS             complement(2376..3578)
                     /locus_tag="KR314_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH0329726.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIVGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRTDNGT
                     LIVFYVKE"
BASE COUNT         1231 a          691 c          387 g         1384 t
ORIGIN      
        1 atataatgtt tttagttttt gataataatt acattaactt gctaatttat gagcattttg
       61 ctcttttata tccttagctt ttttaaaaaa taaaagtcca aggattattg caactacaat
      121 acctattcca ttagatatat taactggaag tctaaatcct tcttttgcct gcattatata
      181 actaaatgtt actacagtca taaaaagtgc tggcaatgta gttatgtaat gatttttatt
      241 tgctttgact aaatatgcag aaccagtcca caacattatc atagcaagtg tttgatttgc
      301 ccaagagaaa tatctccaaa ttatattaaa atctataaat gtaagggcaa cccctacagc
      361 aaataatggc aaagctatta aaaatctatt tttaactgca tcttgattat aattcattgc
      421 atcagctatt gttaatctag cacttctaaa tgctgtatcc cctgaagtta taggacatgc
      481 tacaacacca agtatggcca aagcgcctcc aataggtccc attaatccaa cagatactgt
      541 atttacaaca gttgctgctc caccatcatt aaatattaca ttaagctgag gtataccatg
      601 gaaatatgac atagctgctg ctgcccaaac taaagctatt ataccttctg caaccattga
      661 cccataaaat actggtcttg cctcactctc tttctcaaca catctcgcca taagtggtga
      721 ttgagttgca tggaaacctg atattgctcc acatgctatt gttataaata agtatgggaa
      781 taaaggtgtt ccatttggat tcatattttg taaatttata tttggtatgt tatacccctt
      841 tattattaat cctccagcta cacctactgc cataattaat aatgctgctc caaataaagg
      901 atatatttta ccaatcaatt tatctattgg taaaacagta gccataatat aataaagtat
      961 tatcactcca acccatattt gtgcgtttac acctgtcaat gtctttaata atcctgctgg
     1021 acctgttaca aatacaactc caaccaatac taaaagaata acactaaata ttctcattgc
     1081 tatttttgca ccatttccta gataaagacc taccaactct gatgcagatg tacctttgtt
     1141 cttcattgat aaatatcctg aagcaaaatc gtgtactcca cctgcaaaga ttgttccaaa
     1201 cacaatccac aaaaatgctg atggtccaaa taacgcccct tgtatagctc caaatatcgg
     1261 acctaaccct gctatattta gaaactgaat taaaaagatt ctcttccatt tcattggcac
     1321 ataatctact ccatccctac aagctatagc tggagtttgg tttttgtcat tgattccaaa
     1381 aaccttttct acaaaagtac catagataaa ataacctact ataagtacta ctattgaacc
     1441 taaaaagctt accataaatt catcccctta gtttttatat aattgcttta taaattttta
     1501 tgagtttaag aaaactattt ccagttcaaa tagtaatact tttttatgct aatataaata
     1561 ttaattacat taagtgcata tttaagtaca tgaaatgact ttatatatgc attaacagcc
     1621 taaattcttt tactttgctt ctgcttacag ttacttcata gtcactattt tttaatttca
     1681 atatgtatgt attgttaaac catggtataa tttcttttac tttggttaaa tttacgatat
     1741 aagacctatg tgttctaaaa aatgttttat catttataag attctctact tctgatatgc
     1801 cttcttttaa tataaacttt tctttctcag tatatataaa tgtctgcctt tcattagctt
     1861 cacaataata tatatcatca atatctatta caaccaattt atcacctttc caaaggctaa
     1921 ttttatgtgt tatttcttct atctcctgat ttactgtctc ttttttatat ttatataaat
     1981 tactattgct attagacagt tcaatatcac tcatctcact tttttctaat ttattcaaca
     2041 tagaaataat tctctcttca gaatatggct tcagtacata atcaaataca tctaaagaaa
     2101 aagcatctac tgcataattt tcatacgctg ttataaaaac aattttaggc cttgatttaa
     2161 actgatttaa tgtttttgca agaagcattc catcgagatg aggtatattt atatccaaaa
     2221 aaattacatc tattttattt tcctgaataa agtctaacac ttctatacca ttagtaaatt
     2281 catttactac ctctatgcta cttttattct caataaaata tcttaattcc tttcttgccg
     2341 gaaattcatc ttccactatt atagctctca tacttctact cctttacata aaatactatc
     2401 aaagtcccat tgtctgttct tcttatatta agccctcttc catacatcag tttaagcctt
     2461 aaatgtacat tataaagtcc aatcttattt tctggcatat tatcttgata tacattattt
     2521 ataatttcct cacttatacc tataccatta tcctctattg atattctcac agtattttct
     2581 aatgaataag gtaacttttt tatggatatt ttcactacac cagctctacc actttctaaa
     2641 ataccatgta ttatcgcatt ttccactaat ggttgtataa tcaaacttgg aatctttata
     2701 tttacatctt ctatatcata agatacagtt agtaattctc caaacctagc tttttctatc
     2761 tctacaaaag atttaacttg ttcaatctct ttatttatat caattaagtt atcactatat
     2821 tcaagattat atcttagata agttgataaa tttattatta actctctagc cttatctgga
     2881 ctaaatctta taaatgaagc tattgtgttt agggaattaa acaaaaaatg cggattaatt
     2941 tgtctttgaa gtgcttttat ttctgcttta tttctcgctt ctatatcttt tttatctttc
     3001 tcaatcccct ctactataga tataacaaat cctattccaa tctgtccaac aattaaaggt
     3061 aaatatatcc caacaattat ttgaattgct aaagaatgtg gatatgatat taagtatata
     3121 agtagtatag taaaactttc agaaatcata cctaccaata taccatacat tactctatgt
     3181 tgctttggta tcctcttata tagaaatcct gataatacac ctgctagtat acttgataaa
     3241 agacatggaa cagatgttat ccccccaata tccataaaat atctatgaat ccctgaaaaa
     3301 actcctgctg taatacctac cattggacca aataaaatgc cacctgatac tatagatatt
     3361 attctagtgt ttactattga ccccatataa tttataccac tataagttcc aaatatggct
     3421 aaaaaagtaa aaactaatgc tatacagacc aagtcattaa aactatattc ttccttttga
     3481 aatatctgct taaatatttt taactttgtt attagaagaa acagtactac aatgaggctt
     3541 gtcttttcta ttaatgcgat taaaattgat gttttcatat tccaccttta aaaaataaat
     3601 ttttacacac tctaaattta ttatgtttgt tgtaacttat atatttgaag taatcctatc
     3661 tcgacatttt ccacatttaa ccttttaatt aaa
//
LOCUS       DAEPLF010000278         3601 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEPLF010000278 DAEPLF010000000
VERSION     DAEPLF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.279"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            1..666
                     /locus_tag="KR314_003370"
     CDS             1..666
                     /locus_tag="KR314_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0329727.1"
                     /translation="MVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVERIVLDC
                     IILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKPYSLK
                     ELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEILLFLA
                     ENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWGKGYK
                     FVP"
     gene            738..2753
                     /locus_tag="KR314_003371"
     CDS             738..2753
                     /locus_tag="KR314_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH0329728.1"
                     /translation="MKKYNILKGTLIVLIVSLLAIAGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGEDKLLSLELPEIFSASEVWINGEIVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKDTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKLYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDSSY
                     FMVVLCIICINGSISKIEEDKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFIIVILFSLMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KKFLADVAHDLKAPVSAIQSFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNDFIKEIYENNLPDTQACGVNFETYILEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKVSDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            2803..3483
                     /locus_tag="KR314_003372"
     CDS             2803..3483
                     /locus_tag="KR314_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0329729.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKEISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILTDGDGWTLCKKIRNVTTCPIVYMTYINEEQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
BASE COUNT         1415 a          365 c          622 g         1199 t
ORIGIN      
        1 ctggttgtag atgatgatgt tgatatttta aatataaatc aaatttattt ttctaatata
       61 ggttttaatg tatatacatc agagactgct aaagatgcta tggatatagt agagcgtata
      121 gttttagact gtataatttt ggatatttca ttgccagata tagatggata taaaatgtgc
      181 aatgatataa aagaaaaggt taacatacct ataatatttt tatctaatta tgagcatgaa
      241 gaagaaaaag ttcggggatt tttagctggg ggggatgatt atataacaaa accttatagt
      301 ttaaaagaac ttgaacttag aatatatgct cgtatgaggc agtacaaaaa tattacatca
      361 gttcctaatg tgttaaagtt ttcagcgtta gtaataaatg taaattcaag aaaagtaact
      421 tttgatggag aaagtataga tttaactact atggaatttg aaatattatt gtttctagct
      481 gaaaacaaag aacaagtatt ttcgaagata gagatatata accaagtttg gaaaatgcct
      541 gatattggag atcaacatac agtgcaggtt catattgcac agatgagaaa aaaaattaat
      601 tctttaagca gggagcatca atacattcaa accgtatggg gaaaaggata taagtttgta
      661 ccataagtga gatactgtaa ttaataaata gttcttgttt tatttaatac tactatgaaa
      721 gagatgtggt tgatgcaatg aaaaagtata atatactaaa aggaactctc attgtattga
      781 tagtctctct gttggcaata gcaggattaa atcttctgta taaatatgat aataaatata
      841 catataagtc tattagagca agtaatggaa ttttaactat agataatgat actttcacta
      901 aaggtaaact tgtctttctg atagatgact gggagtttta taatcataaa atatttaaac
      961 cgaatgattt tacagagcaa gatataaaac cagaatatgt ttttataggt caatatccag
     1021 atttttctat gagaaaaggg aatcagtcac catatgggaa tgctacttat cgtatgagaa
     1081 taaaaaatga aggtgaggat aagctactaa gtttagagct tcctgagatt ttttccgcaa
     1141 gtgaagtatg gataaatgga gaaatagttt ctaaattagg agatgtagga acagaaaaat
     1201 atagacccaa aattaaaaat tcagtagtaa gttttattgc aaagaaagat acagaaatta
     1261 taatcaatgt atcgaatttt tcacattatt atagtggttt gtattatccg cctgcattag
     1321 gagaaacaaa ggacataagt aatatgatgt tttatcgatt acttttttat agtataattt
     1381 gcttcacaac ttttgcaata gctatatttt cgttgtctgt atggtttctt tcaaacaaaa
     1441 gtaaacttta cttatatttt ggatgtatgt gtgttttctt tgccatccat gtatcctatc
     1501 cctttgtaca tttgttagga ctgcctttgg taagctttac atatgcggtt gaagattcgt
     1561 cctattttat ggtagtttta tgtattattt gtataaatgg aagtatatcc aaaatagaag
     1621 aagataaagt gtatagattt ttaattttac cattaggaat tacaatgagt atcatatcta
     1681 tagtgatacc aattattctt cttccatatg atatgtactt tgtcaacata tatgggaaat
     1741 taatagacat atataaatat attgtattta tatatatatt aatgtcttcg ctggtttcaa
     1801 tatataaaaa gacatgctct gagcatataa gttcttatat tttagtttca gtaaacactg
     1861 tttttggaat tagcatatta tttgatgcag ttactagtaa tagatttgag cctatattta
     1921 cagggtggca aacagaatat tgtggattta taattgttat tttattttct cttatgatga
     1981 taaacaggaa tagaataata ataaaagaga atgaaaaact tacaaggaat cttgaacatg
     2041 aagttgaagt gagaactaat gaacttacaa cattgttaaa tgaacgtaaa aaattcttgg
     2101 cagatgtggc acatgattta aaggcacctg tttcagctat acaatcattt atagacttga
     2161 taaaggttgg taatattcat gtagatgaag agacaagaac ctatctttat gctataaatc
     2221 aaaagtcaaa tgaagtacaa aatagagtac gtagtttgca agaatttaca tcacaagata
     2281 aaagcattgg tattagtgaa aaaatatgtc taaatgattt tattaaagag atatatgaaa
     2341 ataacttgcc agatacacaa gcatgtggtg taaactttga aacttacata ttggagaaaa
     2401 aaatatatgc aaagataaat aaagagatgc ttacaagagc atttgagaat ttaatttata
     2461 atgctttgac atttactcca tttgatggaa agataatttt atcaatggat aaagaagatg
     2521 gatttgcaat tattaaagtt tctgataatg gaaaaggtat acctccagaa aatataccaa
     2581 aggtatttga cagattcttt acagaaagag ataataatga gccaaaaggg caagggctag
     2641 ggttatatat tgtcaaatcc attataagag agcatggcgg agaaatcttt gtagattcag
     2701 aactaggaaa aggaactgtt tttacaataa aattaagtct atgtttttta taataatagc
     2761 ttatttaata taaaagaata atgatatttg ggagtgatta taatgcaaac taagatattg
     2821 ataatagatg gagacaagga taattgccag aagctaaaag gatttctgga ggaaaaagaa
     2881 ataagtatag atttagcata taattgtgaa gaagctattg gaaaaatatt tagcaataaa
     2941 tatgatttaa tatttttaga aataattcta actgatggtg atggatggac tctttgtaaa
     3001 aaaattagaa atgtaactac ttgtccaatt gtttatatga cttatataaa tgaagaacaa
     3061 agcattttaa atgctttaaa ttctggcgga gatgattact taataaagcc tctaaattta
     3121 gaaatattat atgcaaaagt aaaagctatt ttaagaagaa tgaactcata tgtaaataat
     3181 aatgaaaata atacaaaatt agaggaatat aatagaacta gtggagtaat aaagttagaa
     3241 gacaaaataa taaaattgac tccaacagaa aataagctgt taaacttttt tatagagaat
     3301 cctgaaaaga ctctgactac taaagagata tatgaacatg tatggatgaa tgagtactta
     3361 gaggataact atagtgtggt agttgcagtg aatggattaa gaaagaaaat agagaaagat
     3421 tataaaaatc ctcagaaaat tataacaata agaggagcag gatattattt taataaagaa
     3481 taataaatat attaatctca ttaataattg ttataaatgt gaaatgctaa cgatatctaa
     3541 actaaattat tataaaatag ctgactgtaa tttaattgat aaagcctatt cttctagact
     3601 t
//
LOCUS       DAEPLF010000279         3598 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEPLF010000279 DAEPLF010000000
VERSION     DAEPLF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.280"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            314..2047
                     /locus_tag="KR314_003373"
     CDS             314..2047
                     /locus_tag="KR314_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0329730.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDVFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   2439..3125
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2439..2467
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
BASE COUNT         1441 a          479 c          462 g         1216 t
ORIGIN      
        1 tagttgaatt ggataaaaaa atgtataaaa acaaggagaa aaacaagaaa aaatttgata
       61 aaaatgatta ttaacattgt tattttaaaa taaattaaaa ttatctttct ttttttaaac
      121 ttgtacatta gtttataaat atgtatctat acaagatatt gacattaagt actgaagcat
      181 atattgtttc agttcttttt tgttaaaatt aacttatttg acaaagatat catagtttaa
      241 caaagctttt aatttaaata tattgatgta taatataatt aaaatataat agcaaaaaga
      301 aaggaggaat atcatgagca tactcctaaa aaaagcaccc aaattagcaa aacatattat
      361 aacaagtttt tatataaatc gagatatcaa tgaagtatta aaataccttt gtgaaaatgt
      421 tacatggatt ggaccaggag aacaggaatt tctcacttct tttaatgaaa taaaaaatta
      481 tttttatgca ggtcaagatg aaattccttc ttgtgatata aataatgatg tatttgaaac
      541 tgtaagtgaa gatgagaata gatgtatggt attaggaaga tatactgttc gaactaaaga
      601 aaatgctcaa atgattttag aggtaaatca acgttgtaca tttgagataa ttgaggatag
      661 aggaaaatta ttagtaaagc acatgcacat atcaaaccct tatggtgaaa tgcaacttga
      721 tgaatacttt cccactaaaa ttggaactca aagttatgat tatttgcaaa gattactaaa
      781 ggaaaaaaca gaagttattg aaatgattac aaataatatc aatggtggct taaaaggtag
      841 caatgatgat agtacatact catttttcta tgtcaatgag ggattgccca aaatattagg
      901 atacacatat gatgagttca tggaaatgag tggtggttca gcagtgggtg ctgtatatcc
      961 accagactta cctaaagcac tagaagattg tgaacgatgt tttgcaaaag gacctacata
     1021 ttcatctgaa tatagaatcc gaaaaaaaga tggaacatta atgtgggtat tagattctgg
     1081 aatgaaatcg ttaaatagtg atggcatagt aaaaattaat agtattatta ctgacataac
     1141 ccaactaaaa aacatagaat ctgaactaaa attagaaaga gagcgatatc gtattgcttt
     1201 gcaaaatatt acagatatca tgtttgagta tgatattgaa aacgataccc ttataaaata
     1261 tcaacgtgta gaaattgaca aaaaaattga attagaaaat tttgaaacaa aaaattactc
     1321 caaactatta gaatctggta aaattattca ccttgatgat attggaaaac tactagaagt
     1381 acttcatgga aatcttcgtg aaacaattga gataagacaa gtaaattcat taactaaaaa
     1441 tgaatggaga tggattcgtg ttcaatgttc agttatatat gatagtgacc ataaccctat
     1501 caaaacaata ggcgttttaa aagacataac tgaagataaa tctaaattag aaagcttaat
     1561 taatcaggct caacgtgacc cactaacaca attatataat caaagagtat cacaaaatct
     1621 tattgaagag tatctatgta gttctgattc caaaaataat gatgctctat tagttattga
     1681 tattgatgat tttaaaactg taaatgatac ttttgggcat ttagagggaa atgaagtttt
     1741 agttgctgta tcaaaaatac tattacataa cacacatgat aaagacatag ttgctcgaat
     1801 tggtggagat gaatttacaa tatttataaa atcattaaca aaggatttaa taataaaaat
     1861 tactaatgat attttaaatg atgcaagtaa aataaaagta aaggacaatc ataaaataac
     1921 attaagtatt ggtattgcct tcactgatga ttcaactaaa ttatataaag atttattttc
     1981 taaagctgat aaagctcttt atctatcaaa agcagatggc aaaaattgtt atagtacata
     2041 cgaatgataa agctatttat acgaccaaga atgaaccttt agatttaatt tcatagtcta
     2101 acaatcaaag agttgaagct aatcttatag ctagtcaact agttcctaga aaaaatttag
     2161 acaatatatt tattgatact gctcattttt ctaaagatca aatttatata aaaaattaaa
     2221 cttttatgta catacagata ctaatgataa acatttagca ttagtatctt ctatcttaaa
     2281 ataaaatgat aatttatcta tataaaaaag tgctttgttg acagagttca cttttttata
     2341 taggtacaga atttaagtat aaatacatgt tttattctat acattttagt taatgtaaaa
     2401 tttcttgatt gtactctcta acttttcaac attatactat ttacatacca cttagttaat
     2461 ataaaaccaa aagttggaga aatagaagtg ccattcacat tcgaatttac ataccactta
     2521 gttaatataa aacactttaa taattctacc tttactagtg ctaaagtaat ttacatacca
     2581 cttagttaat ataaaaccag gaacatatgg caaactataa ggaaaacctg caaatttaca
     2641 taccacttag ttaatataaa accctctgtg actccatatt ttttcataat atataattca
     2701 tttacatacc acttagttaa tataaaacat agaagttgtt gcatataact acattgacaa
     2761 gttattattt acataccact tagttaatat aaaacttttc tactttttta atcatttaat
     2821 cacctgctta aaaatttaca taccacttag ttaatataaa acttttgata ttctaaagaa
     2881 gtgacaatga tgtgacacaa atttacatac cacttagtta atataaaact attatgtagt
     2941 catatatttc aaactctaat ttcgtattta cataccactt agttaatata aaacccattt
     3001 gacccatgca tattgacttc caccttcctt atttacatac cacttagtta atataaaact
     3061 ttacaatata gacgtacata tacttctgaa aaacctcttt ccataccact tagttaatat
     3121 aaaactttac cccaaaataa actgggcatt ttcaatactt acacatgtgc aactctctta
     3181 aatttgcagt gaaccatgag tagtgcaaat gataaagttt atcatatacc ctcaacacct
     3241 tatatttcaa gcattaagcc ctactttgta acaaatatcg ctcactgcaa aactatttac
     3301 atttttatta tatcataaat atattatttt tgaatatctg taccaatttg tggtataata
     3361 aaaacaagaa gaactacaat ctatttagac gtagagtgaa gttcaaatac taatgtttat
     3421 tctttttgaa cttgattttt agttcaaaac taaagtcact cctgctgaac tatacccact
     3481 gttttataca gttaaaacat gtaaaagtag ttataaagta ctagtgtaac tcattagtac
     3541 tttttttatt atattcatat aattaatatc tatattttaa agtttactat ctaaaaaa
//
LOCUS       DAEPLF010000280         3559 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEPLF010000280 DAEPLF010000000
VERSION     DAEPLF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.281"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..139)
                     /locus_tag="KR314_003374"
     CDS             complement(<1..139)
                     /locus_tag="KR314_003374"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBH0329731.1"
                     /translation="MEGFWRILKVEMYYLRKLDTYEQLVKTIEDYIYYYNNFRYQKRL
                     NS"
     gene            complement(169..558)
                     /locus_tag="KR314_003375"
     CDS             complement(169..558)
                     /locus_tag="KR314_003375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBH0329732.1"
                     /translation="MRAVGLSSVCRKKKFSYVKCTPEVIAENVLSRKFSADKTSQKWL
                     TGVTEFKLTNGMKAYLSAILDLDDRSIVSYVIGKSNNNNLVLETFDKAIELYPNAKPI
                     FHNDKSFQYTSKVFKSKLLTQGMIQSM"
     gene            complement(570..1030)
                     /locus_tag="KR314_003376"
                     /pseudo
     CDS             complement(570..1030)
                     /locus_tag="KR314_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1459..2469
                     /locus_tag="KR314_003377"
     CDS             1459..2469
                     /locus_tag="KR314_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329733.1"
                     /translation="MITKIKKTYIYLKEIQKIYKINEHKELVHIIKKLINDNKIKPIK
                     TSDLTPQHEQLYTKYRIIKQETEEDKKILEEINYKICDKLSIDYYRKNLVHYKNNRVA
                     IMDLNSYLIKKSDNLSKKISINERSYEIFKDEKFISSKVGKEVLKNLKIDLIKDLNVY
                     KTPEPFIYLSINRISPQKILIIENKDTYITITKMLLEGKEILKNKIDTVIYGEGKKII
                     NSIKGLKEDITIEYLTNKENEFLYWGDIDKTGLSIYTSLKDTNEINNIRLFEVAYEHM
                     IDKTKENTLRNIPKNQKDNFECGLENINNLNLKESIKNILVQGKYIPQEALNRFDLEE
                     YN"
     gene            2469..>3559
                     /locus_tag="KR314_003378"
     CDS             2469..>3559
                     /locus_tag="KR314_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329734.1"
                     /translation="MEMINILEGYNKRVNCIGSYYLIATLANNRNKFKEFDNIQFYNL
                     LIQVLCYIFDRSLRRKNCLRDDIKDFIEEINRMDYKIMLSEDDLKDLANYIINGLTNS
                     GKVYLFTYYSLEQEKHIDESIKIIEDKNVKINNQERLSYSLTTEGYRLLLSTKEYDEL
                     FQIQISQMIAKLRIEKGDYQGAKEDIYDIINTLEIHQQKIDNYIKIIKVNILYIKEEK
                     YSTVIDTTVETLLEEMDKYEELKKEVKLLIKDKEDYLEKDEDIDKEKIKNIRTQLLQM
                     KELIDGIVLAKSTATNLLSRVQDFREEFKEILKKLLTTPVIKRFNFKEEILDRLEKDS
                     DFLLIIRELYISLFKVELPNRFNIGVAYQ"
BASE COUNT         1481 a          436 c          454 g         1188 t
ORIGIN      
        1 tagaatttaa tctcttttga tatctaaaat tattatagta atatatataa tcctctattg
       61 ttttgactaa ttgttcgtat gtatcaagtt ttcttaaata gtacatttcc acttttaaaa
      121 ttctccaaaa tccttccata ggagcattat caatacaacg agcaactctt acatactttg
      181 tatcatgcct tgtgttaaaa gttttgattt aaatacctta ctagtatact ggaaactttt
      241 atcattatga aaaataggtt ttgcatttgg atatagctct attgctttat caaaagtttc
      301 taacaccaag ttattgttat ttgactttcc aataacatat gaaactatac ttctatcgtc
      361 taaatctaga attgcgctta aataggcctt catcccattg gttaatttaa attcagttac
      421 acctgttaac catttttgag aggttttatc tgcactaaat tttctactta atacattttc
      481 cgcgatcact tctggcgtac atttaacata actaaatttc ttttttctgc aaacagaact
      541 taagcctacg gctctcatta gtctgtacac tctctacaat ctttaacaat ttcaactctt
      601 tcatctaatg ctgtttttct agtctccata aagatttctc ccttcgaggt ttcttttatc
      661 tacttatgag gattatactc tttaccccaa gcttttagtg aataaactga tctcatttta
      721 tatttttttg agattgtgtc ataagatcct tcaccattta gataagattt tatagcattc
      781 aatttaaatt cttcacaata gcataattta caactttgtt ttattaaacc atcatttcca
      841 aaagcttcat aatttcgcat ccactctctg aatgtacgtt tatccaatat ttaattcttc
      901 cattattttt attactgtat tctcaccttt taaatatgat tcaattgctt cgattttttt
      961 gctctgaaga tatcttagtt ttagatagtc ttacatattt taactgtctc ctaagatgag
     1021 tataattcat tcatcatcag ttctatagaa tacacctagt tcctacttac tgtaggaact
     1081 aggactatat gaaacccatt catagattgt agtttcttct gttgcattat agcgcagaat
     1141 agtaaattca tgtgaaattt tattttgaat ttactattta tatataaatg aagagtggtc
     1201 ttattttcaa ttaaaatttg gaacaatttt gggttgacat ctacaattaa ggatttaagt
     1261 ttttgttata ctaataaata aaaacagaga ttcaaaatat ataaataata cttaaactag
     1321 tgctgataga ttttctttaa gttctagttt ttttattgtc attatctaat atgtactatt
     1381 ttatgtacag tcaatataat ctgaaattgt aaaatggtgt ataatatagt tataagtaaa
     1441 gaagggattt aaacatgtat gattacaaaa ataaaaaaga cttatatata ccttaaggaa
     1501 atacaaaaaa tatacaaaat caatgagcat aaagaactag tacacataat aaaaaaacta
     1561 ataaatgata ataaaataaa accaataaaa acctcagatc taacacctca gcatgagcaa
     1621 ttatatacaa agtatagaat aataaaacaa gaaacagaag aagataaaaa aatattagaa
     1681 gaaataaatt acaaaatatg tgataaatta agtatagatt attatagaaa aaacttagta
     1741 cactataaaa ataatagagt tgcaataatg gatttaaata gttatcttat taaaaaatct
     1801 gataatctat caaagaaaat atcgattaac gaaagatctt atgaaatatt taaggatgag
     1861 aaattcataa gtagtaaagt aggaaaagaa gtactaaaaa acttaaaaat agatctaata
     1921 aaggatttaa acgtatataa gacaccagag ccattcatat atttatcaat aaatcgtata
     1981 tctccacaaa aaatattaat tatagaaaat aaagacactt acataacaat aacaaaaatg
     2041 ctattagaag gaaaagaaat acttaaaaat aaaatagata ctgttatata cggagaagga
     2101 aaaaaaataa tcaacagtat taaaggatta aaggaagata taacaattga atatcttaca
     2161 aataaagaaa atgaattttt atattgggga gatatagata aaactggatt atcaatatac
     2221 acttccctaa aagataccaa tgaaattaat aatataagat tatttgaagt tgcttatgag
     2281 catatgatag ataaaacaaa ggaaaatacc ttgaggaata tccccaaaaa tcaaaaagat
     2341 aattttgaat gtggattaga aaatattaat aacctaaact taaaagaaag tattaaaaat
     2401 attttagtac aaggaaaata catacctcaa gaagctctaa atagattcga cttggaggaa
     2461 tataactaat ggaaatgatt aatattttag aaggctacaa taagagagta aactgtatag
     2521 gatcttatta tcttattgca actcttgcta acaatagaaa taaatttaaa gaatttgaca
     2581 acattcaatt ttacaattta ttaattcaag ttttgtgtta tatatttgat agatctctta
     2641 gaagaaagaa ctgtttaaga gatgatatta aggattttat tgaagaaata aatcgtatgg
     2701 attataaaat aatgctttca gaggatgact taaaggattt agcaaactat ataattaatg
     2761 gattaaccaa tagtggtaaa gtatatctat ttacctacta ttctttagaa caagaaaaac
     2821 atatagatga aagtattaaa attatagaag ataaaaatgt aaaaataaat aatcaggaaa
     2881 gactatccta ctccttaaca acagagggtt atagattgtt gttatcaaca aaagaatatg
     2941 atgaactatt ccaaattcaa atatcacaaa tgattgcaaa gttaaggata gaaaagggtg
     3001 attatcaagg cgcaaaggaa gatatttacg atataataaa taccttagag attcatcaac
     3061 agaaaattga taattatata aaaataataa aagtaaatat actatatata aaagaagaaa
     3121 aatattcaac agtaatagat acaactgtag aaacactttt agaagaaatg gacaaatatg
     3181 aagaattaaa aaaagaagta aaactattaa tcaaagataa agaggactac ttggaaaagg
     3241 atgaggatat agacaaagaa aagataaaaa atataagaac tcaactatta caaatgaaag
     3301 aattaataga tggaatagtt ttagcaaagt ctacagccac aaatttgtta tctagagttc
     3361 aagattttag agaagaattt aaagaaatac ttaaaaaact attaacaact ccagtaatta
     3421 aaagattcaa tttcaaggaa gaaatcttag atagacttga aaaagacagt gattttttat
     3481 tgataataag agaattatat atatctttat ttaaagtaga gttgccaaat cgatttaata
     3541 ttggagtggc atatcaaga
//
LOCUS       DAEPLF010000281         3551 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEPLF010000281 DAEPLF010000000
VERSION     DAEPLF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.282"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            335..2158
                     /gene="typA"
                     /locus_tag="KR314_003379"
     CDS             335..2158
                     /gene="typA"
                     /locus_tag="KR314_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBH0329735.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            complement(2248..3156)
                     /gene="trxB"
                     /locus_tag="KR314_003380"
     CDS             complement(2248..3156)
                     /gene="trxB"
                     /locus_tag="KR314_003380"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBH0329736.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
BASE COUNT         1233 a          568 c          501 g         1249 t
ORIGIN      
        1 tttaccttca ataaatttat gagaccactt aagaagttct tcaaaattac aaacttcttc
       61 ttttataaaa gtatcacttt cacttgcaaa atatttaatt attattttgg ttacttccaa
      121 catatatcat cctttactct tttattaatc tttttatcta ttaagattat tatttgtcta
      181 aaaaattttt ttaaacagaa taattttcag atgcaaaact aacagtttta tccaatttaa
      241 actatattaa tacaatttaa tatattcctc cttgtaccta ataatatatt gtgctataat
      301 taataagtta ttttaatcga agggagcaac aaaaatgtca caaaaacata aaattataaa
      361 tatcgcagtt atagcccacg ttgacgctgg aaaatcaaca ttggttgatg ctttcctttc
      421 tcaaagcggt gtttttagaa agaacgaagt agttaaagac tgcgttatgg acagtaacga
      481 ccttgaaaaa gaaagaggca taactatata ttcaaaaaac tgtgcaataa actatgaaga
      541 ttacaaaata aacatagttg atactccagg acacagtgac ttttcttctg aagttgaacg
      601 tgttatgaaa actgttgaca cagttattct attagttgat gctagtgaag gcccaatgcc
      661 tcaaactaga ttcgttttac aaaaatcttt agagttcggt ttaaaaccaa tattatttat
      721 aaacaagatt gataaaaaag accagagagc ggaagaagta gttaatgaag ttttcgattt
      781 atttgtagac ttaaatgcaa cagatgagca atgcgaattt cctataatat atggtattgc
      841 taaacaaggt attgctaaac ttgaaatgga tgatgatagt gaagaccttt ctcctctatt
      901 taaaactatt gtaaatcacg ttgaagctta tcctaattac gataatgaac ctctacaatt
      961 ccaaatatca gctcttgcat atgatgatta tgtaggtaga cttggtatag gtagaattta
     1021 caaaggtact cttaaaaaca atacacaagt tgctatatgt agagaagatt ctgttgtatc
     1081 taaaggaaaa gtatcaaaac tttctgtgta tgaaggttta aaacaagttg aagttgatga
     1141 agctactagt ggagaaatag ttgttatagc tggtattcct gatatatcta taggtgaaac
     1201 tatatgcgat ttagatagtc cactacctat ggaaatgata aaaatagaag agcctactct
     1261 ttctatgaac ttcttagtaa acgactctcc attcgttgga aaaagtggaa aattcgttac
     1321 aactagacat ttgaaagata gacttgaaaa ggaacttgaa gtaaatgttg gtcttaaagt
     1381 tgaacctctt gatactactg atggatacaa agtttctgga cgtggagagc ttcacttatc
     1441 tatactactt gaaaatatga gacgtgaagg ttatgaagtt ggtgtttcta aacctgaagt
     1501 tttaatgcat aaagaagatg gaaaactaat ggaaccaata gaaagagttg ttgtaaactg
     1561 tcctgaagtt tactctggta ctattataaa tgaattaaac atgagaaaag gtatgatgga
     1621 gtctatgtca atagaaggag actatgtaaa aatagagttc ttagcaccta ctcgtggtct
     1681 tttaggatat cgaagtgaat ttataaacgc tactcgtgga gaaggtactt tagttcgttc
     1741 atttgaaaaa tttgaagaat ttaaaggaga aataccttca agaggaaatg gtgttttaat
     1801 agcacaagga cctggtgtta ctatggggta ttctcttaat gccttaagtg atagagctgt
     1861 aatgtttgta gaccctggtg tagaagttta cgaaggtatg ataattggta tgaattccag
     1921 aaaagatgat atggtagtca acccttgtaa aaacaagaaa atgagtaacg tacgtgcttc
     1981 tggttcagat gatgctataa aattatctcc tcctagaata tttactcttg aagaagcact
     2041 agagtttata gaagatgatg aattagttga aattacacct gattctataa gacttagaaa
     2101 aagattctta aatgaacatg atagattaag atataacaaa tctagacaag gtaaataaaa
     2161 cgtacattta cctctaatta acaaaatagc catatagtac tgaaaaatta tttcagttta
     2221 tatggctatt tttatttgaa atttatatta ttcaacattt atataattag ctgcttgaac
     2281 ggctgcaata gctccatctg atgctgctgt tattatttga cgtaaaaact tagttctact
     2341 atctcctgct acatatacac cttctactga tgttgtacaa gactcatctg aaattatata
     2401 tccaccttca tccaaatcaa gaacattttc aaacatttta ttattaggtt taagacctat
     2461 agcaataaaa ataccactaa catctatagt tcttttttct tcagtcttta agttttttac
     2521 ttctattttc gatacagtct tttctccttc tattttttct acaccatgac tgtaaagtat
     2581 ctcaatattc tctcttgctt tcacagattt ctccaacact tcttcacctc taaaactatc
     2641 tcttctgtga attaaataca cttttgtaca attattagct aaaaataaag catcttctag
     2701 tgcagtattt cctccaccta ctatagcaac ttctttatct ttaaaaaagg ctccatcaca
     2761 tgttgcacag taagatactc ctctacctgt aaattcttgt tcccctgcac aacctaattt
     2821 acgtctttca actccatttg caagaataac agcctttgct ttatgttcac ctgaagaagt
     2881 ttttacaacc tttacttttc cctctaaatt tatctcttca acctcatcaa actgaatatc
     2941 aacaccttga gcaactgctt gattgtatat attattagaa aattctaccc cagaaatctt
     3001 ttgaactgct ggataatttt ctacctcaga ggtacttgca acttgacctc cataaatatt
     3061 tttttcaaat actgtaacgc ttaaccctgc tctcatagca taaattgctg aagttaaacc
     3121 tgctggtcca gcaccaatta caatgatatc taccataatg tttacctctc tttatattat
     3181 tatttcttat atattattct catcattctt atactttact attattatcc atatatacta
     3241 caaacaaaac attatttttt attaattaac tttattttac atatatataa aaaaactgca
     3301 aataaaaatt aacttttatt tacagttctc acttatttat ataattcaaa accttattgg
     3361 ttatcattta cagtgtcttc taatatattg ctttctgata tatatgatag aattgaaagt
     3421 ccatacataa ccaaaactaa tgctgctgtt ataagtacta ttcccatatt taatacctcc
     3481 ttttgtttac agtatactat tattatgtga aatatttatg aatttaattt gaatatatat
     3541 gtaatttcac a
//
LOCUS       DAEPLF010000282         3529 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEPLF010000282 DAEPLF010000000
VERSION     DAEPLF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.283"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..527
                     /locus_tag="KR314_003381"
     CDS             <1..527
                     /locus_tag="KR314_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329737.1"
                     /translation="REAFSLSFLLDSKGVIDKNSVLNNKDYSISTLALNQQIMILEKV
                     EELLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVF
                     DKEIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDIHRLDKMVYDLKLNSLEND
                     IPLLKKIFGKRRRF"
     gene            528..1664
                     /locus_tag="KR314_003382"
     CDS             528..1664
                     /locus_tag="KR314_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBH0329738.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            1728..2804
                     /locus_tag="KR314_003383"
     CDS             1728..2804
                     /locus_tag="KR314_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBH0329739.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHSITGILIPKANSELIENCIEALYSMNLQDMYVI
                     PIIETREFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            2782..>3529
                     /locus_tag="KR314_003384"
     CDS             2782..>3529
                     /locus_tag="KR314_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HBH0329740.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGVRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELNCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIY"
BASE COUNT         1418 a          359 c          627 g         1125 t
ORIGIN      
        1 cgagagaagc ttttagttta tcctttttat tggattcaaa aggtgtaata gataaaaact
       61 ctgttttaaa taataaagat tattccatat caacactagc acttaatcaa caaattatga
      121 ttttagagaa agtggaagag ttgttagaag gaagtatgtt tttgaatggt ttaagtggag
      181 aagacaagat aaaaggtcta tttacagtac ttcacatgga taagaaattt gttcatatga
      241 tgaacaattt cgattactca cttataaacc caaaattgat tatatacatg tacaagagta
      301 ttgtatttga taaagaaata gtttttctta tgcttttact tagtaagatt ggatttgata
      361 ttatcatatt atgtccagga ggagaaaaca atatagagaa tgttataaat aaccaattga
      421 ttgatataca cagattggat aaaatggtat atgaccttaa attaaattcg cttgaaaatg
      481 atataccttt acttaaaaaa atttttggaa agaggagacg attttagatg gggataaata
      541 taaatagttt ttcaaaagat aagaactctg tagaggttac tgagaatttg cctacagcag
      601 ttcaagaaga aaacttcgat ataatggaat atacaaataa taaaaaaaat gaactgagaa
      661 agtctaagga agttgaggca ttgacatccc ttatagaagt agaaaatcca gatacgattt
      721 tacagtttgg taggaaagca tctgaaggag tagcaagagt ttctgatagc cttttaaata
      781 caataaaatt aaatagaaat gaagaaaatt ctaaaatgct ggttcattta actaaaataa
      841 tggataagtt tgatttagac gattttcaag aaactaagga accaaatttc gttcaaaaac
      901 ttttcaaaaa agctaataat gccattgaaa tgatgtttca aaagtatgaa acattgggag
      961 gagaagttga gaaaattcaa attgagcttg agcaatacga aagagatata gcactttcta
     1021 ataagcaaat aggtgctatg ttaaatgaaa actttgagtt ttataatgaa cttcaaaaat
     1081 atattgttgc tggagaaatg gcaatagaag aaatggataa tgaaatacta cctttcttta
     1141 aacaaaaatc agagactagt ggagaccaaa tggatgttgt caattatcaa gaactattaa
     1201 aagtttatga tatgttaaat caaagagtct atgatttaag aatagcagaa aatatagcta
     1261 tccaaacaat acctatgctt agaggtatgc aacataataa ttatggttta attagaaaaa
     1321 taaatagtgc atttgtcgta actttaccag tatttaaaca atgtttatct caagctatat
     1381 tacttaaaaa gcaagaatta caagcaaaat ctctaaaagc acttgatgac aaaactaatg
     1441 aattattgct tagaaatgca caaaatgtaa gtactcaaag tgcacaaata gctagaatgg
     1501 ctggaacaag ttctgttcaa atagaaacat tggaaaaaat gtacaatact atcaagtctg
     1561 gtatagatga aactatgaga atagaagaaa acaatagagc tttaattaag gataatacaa
     1621 agagattgga agaattaaat acgactataa tttataataa gtagtattat attaagggct
     1681 aatttgtata aagttggccc tttctatgta tatgaggagg ctagacattg aaatatttcg
     1741 attatatctg caaagatgat ttggagagaa tttttttgaa ggagccagaa gactttagtg
     1801 ctaagactga aaaagatgtg ttgaaatatg cactgggcgc ttttttatac gttccagcta
     1861 ctcaatataa tatgatttat aagagtatta taggggatgt taagggagtt agaccattag
     1921 ctatttgttt agaagatgct gttggagtaa atggagagtt agaggctata gaaaatctta
     1981 gattaatttt aaaaaatatt agcaatgaaa gtataactaa taaagatgga ataccactaa
     2041 tttttgtaag aataaaagat gtggagcaac tgttgagaat taaagagatt ataattaaaa
     2101 atagtcattc tataacagga atattaattc ctaaagcaaa ttcggagttg attgaaaatt
     2161 gtatagaagc tttatattct atgaatcttc aagatatgta tgttattcca ataatagaga
     2221 caagagaatt tatatataat gaaaaaaaag aacttagttt tactaatttg tacaatgcta
     2281 ttttaagaca taagtcaaga atattaagca ttagagtagg cataactgat atattaggga
     2341 tgtatggtat aaggagagat aaaaacttct ctatatataa taatttgatt tgttcttcat
     2401 ttatattaga tataatcaca tatcttcaaa gaccagagtt agatatacct ataagtggtg
     2461 gagtatcaga attttttgat atgacaaata aggatataag aaataagtat atagaagaaa
     2521 tattattgga taaatatcat ggattaattg gaaagaccgt aattcatcca atgcaaattc
     2581 aaattgtaca agcgctatct acagtatcat atgaagattt tactgatgct ttggatatac
     2641 tggatagtac agacagtaag tatggtgtga gcaagggtgt attaggagag agaatgaatg
     2701 aaacaaatcc acatttttta tgggctaaaa aaacattgat tctatctaaa atatatggtg
     2761 tattaaataa aggagtagac tatgaagaat tgcttaaatt ttaattgtga gattgacatt
     2821 attgataatc cattgaattt agaactaaat gattttttag acgtaggagt aagaaataat
     2881 agcaaaagac gttttttgtt tatctcaaag aaactcggta aacatttagc atgtaagcca
     2941 agtgagatgg ataatttggg aaaattaatg gctacaattt ataatgaaaa aaataaggaa
     3001 gaatcaaatg gtactgttat agcttttgct gaaactggta cagctgttgg gcatagtttt
     3061 tttgattact tatgtggaga ttatgaattt attcatacta ctagagagca gtttgacgag
     3121 ttagaaaatc tggaattttt agaggaacat tcccatgcaa cagaccagaa tttatacttt
     3181 ggtatgctta acaactttaa atttggagat gagataatat tggttgatga tgagattacc
     3241 acagccaata catgcatgaa tataattagg aaaattcaaa gcatttatcc taaaaagaaa
     3301 tacactatat gctcaatact caattgggta agtgatgaaa atttaagtaa agttactcgt
     3361 ttagaaagag agctaaattg taagatagat tttgtatact tatttaaagg aaactttaaa
     3421 ttttatatag atgaagatat tatttcaaat tacaacgata attatagtat tactaaatta
     3481 gagactattt caagtgataa agtaaattta gtagtaaatc atatttatg
//
LOCUS       DAEPLF010000283         3512 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEPLF010000283 DAEPLF010000000
VERSION     DAEPLF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.284"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            216..593
                     /locus_tag="KR314_003385"
     CDS             216..593
                     /locus_tag="KR314_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0329741.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            586..1620
                     /locus_tag="KR314_003386"
     CDS             586..1620
                     /locus_tag="KR314_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH0329742.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENAVGNNITQIDISTDNNLMLYVAYLWLVVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDVNCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLTDCSLKDKKEYALALIKSIKLSKNYNSGILTTEFNKTSLE
                     KRLDSIVKKKGKNGILVLLILITLFSVTYFNFESISFDNSNSLRNMGILQKSGVDLKE
                     EDDISMRVIN"
     gene            1686..2273
                     /locus_tag="KR314_003387"
     CDS             1686..2273
                     /locus_tag="KR314_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBH0329743.1"
                     /translation="MKNLKKILIITGCLTLISTSLVFANTTTDVDTSKDDDSIISSCT
                     ITGYEFTYENAPQSVKFDYEKKCEALNIKPSKNDIIFVNKDSIDANDVENYVEPKAYI
                     IRYKKGYVFEVTGPKSYTLNVSNTVVGYNRVESGNPVHLVQLLCRTLDGSSIDDDSEF
                     GPATYNAVKSLQGRLGLTRDGIVGRSTWDAAGRRL"
     gene            complement(2651..3412)
                     /locus_tag="KR314_003388"
                     /pseudo
     CDS             complement(2651..3412)
                     /locus_tag="KR314_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612258.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1310 a          375 c          507 g         1320 t
ORIGIN      
        1 ttttaatgtg taaataacat tctacactat tgtaattaaa tatgtaaaaa gtaaaatatt
       61 taaatagata tattttaaaa tctaactttt aatattgtaa aaaaattaga aatttaacaa
      121 aaagtacaaa aattcacatt tttttcataa agtgtttgac taaaaaattc acatgttgta
      181 gaattaaggt agcaggagtt ttgagggagg tgtaaataat gataagaaaa ataccacaag
      241 cagaactaaa agtaatgaaa ttcatttgga aagtagatgt tacagtgaca tcaaaagatg
      301 ttattgaagc tatggaacaa aaatatggct ggaaacaaac tactacactg acacttttat
      361 ctaggctggt aaaaagagga ttcttagatg ctcaaaaaat agacagatat acacattaca
      421 caataagagt aaaacacaaa gattatttaa gttttgagac aaaggatttc ctaagtaata
      481 tacatgatga ctctttgaga agcttaattt cagcattaca tgatgatgaa aatataggta
      541 aagataagct agattttcta gaaagttatt ttgataactt aaaggatgaa taattatatg
      601 ctatatgttt ttgaaaatct tataaagaca actattgtat gtagtcttgg tatttgtcta
      661 ttattatttt tgaaaagata tttatttaag aagtttagta agagattcaa ttattatatt
      721 tggttgataa tagtctttag aatgttactt tttctattta attatacaat agtgtatgaa
      781 gtaaaagaac ccaaagaaaa tgcagtagga aataatatta cacaaattga tatttctaca
      841 gataataatc ttatgttgta tgtagcttat ttatggttag ttgtaactat agtcattgca
      901 gtttatacat ttataaagta tacaagattt aaaaatctag ttgttgatgt atcttatgat
      961 attgaagata atgatgtaaa ctgtatctac aaaaacttgt tgaaagaatt aaatataaaa
     1021 aagaaaattg aattaagagg ttctgatgaa ttgataagcc cagcaggcat gggattattt
     1081 aaatcttaca tattcttacc agactatcct tatagtaaag atgaactaac ttggattttg
     1141 aaacatgaat taatgcattt taaaaataaa gatattttaa taaagttttt agtgttatct
     1201 gttagaataa tatattggtt taatccactt gtttatgtaa tgagcaataa agtaaatcta
     1261 gattgtgaat tatgttgtga tgaaagtgtt ttgactgatt gttctttaaa ggataagaag
     1321 gagtatgcat tagcacttat aaagtcaatt aaacttagta agaattataa tagtggtatt
     1381 ttaacaacag agtttaataa gacaagtctg gaaaagagac ttgacagtat tgttaagaaa
     1441 aaaggtaaaa atggaatatt ggtgttattg attttgatta cattattttc agttacatat
     1501 tttaattttg aaagtattag ttttgataat tcaaattctt taaggaatat ggggatttta
     1561 caaaaatcag gagtagatct taaggaagaa gatgatatta gcatgagagt tataaattaa
     1621 tagattaatg tatgtaatga aaaggtatat tgtcgtatta ttaataaatt aggaggaatt
     1681 atgaaatgaa aaatttaaaa aaaattttaa taattacagg ttgtctaact ctaatatcta
     1741 ctagtttggt atttgcaaat actactactg atgtagatac atcaaaggat gatgattcaa
     1801 taataagttc atgtactata actggttatg aatttacata tgaaaacgca ccacaatctg
     1861 taaaatttga ttatgaaaaa aaatgtgaag cgctaaatat aaaaccatct aaaaatgata
     1921 taatttttgt taacaaagat tctatagatg caaatgatgt agaaaactat gttgagccaa
     1981 aagcatatat tattagatat aaaaaaggtt atgtttttga agttactgga cctaagagtt
     2041 atacattaaa tgtaagtaat actgtagtcg gatataatcg tgttgaatca ggtaatcctg
     2101 tgcatttagt tcaactttta tgtagaacct tagatggttc aagtattgat gatgatagtg
     2161 aatttggacc tgctacatat aatgcagtta agtcacttca aggaagactt ggattaacaa
     2221 gagatggaat tgttggaaga agtacttggg atgcagctgg aagacgtctt taaaaagaaa
     2281 gctattgcac agataatttt aaattgctga atctgtaaac atttacatgt attctttaaa
     2341 taaaaattta ttttttaagt gataattaaa tttttactaa tagggctatg gctttaaaaa
     2401 tcatagcctt attttgatat cttttccatt tttgcaaaag ctattaaccc tttaagaaaa
     2461 tttcttgttt ttttgaaaaa tttctttctt caaaataaat acttaaatga gctaaaattt
     2521 gagcacaatt tctaattttc tgagtccatt tttaagaaat atatatggtt gttaagtata
     2581 gtgatttgtg caaataatta tccaaagtaa aaatagattt caatttagta acttttctta
     2641 ataatctatt ctaacttccc attgaatttg tagtatatat cgatgtattt atttcttgag
     2701 agtacttaaa atatgttact aattcattcc aattgtttca ccaacctctt aatactattg
     2761 tatattgatt gtactatttt ttctctagtg atagctcact taatgctacc tcttctgtaa
     2821 ttgtagcata tctttcttta aatcttttac agattctttt ctatttttat atgatacata
     2881 atttaaagta tttttaattt gatgcacaac atatctttgt acttaagtat caggaaatat
     2941 cgctttaatt gcattagaaa attcaactag tctatctata gaagctgcta ttaatacatc
     3001 tttaattccc atgtttctag catctataag cacttttagc caatactttg aaatttcatt
     3061 ttctccaatc catattctta aaacctcttt tatgctacat atagctatat agactgtttt
     3121 gtttattatt cgacctcact tctaacttta taatgtatag catctataaa tacaattgaa
     3181 taaattatat taagaggtct aagttaccat ttatgagtta aaggaataac tttatcagta
     3241 gttttagtta ttagtgaatg agataatatc aattccataa gtacttttaa gatgacttaa
     3301 tatattttga gtagacatgc cttttgagta tattccaatc acttgatttt ttattcctaa
     3361 tatatcattt tctatcataa cctaaaagtt cattaagctc cgcttctaac atttaatgga
     3421 ttgtactttc aaataaatct tttagtatat cttgaatatc tgtaattgtt ttggcatcat
     3481 attcttcaat aaattttttt aatattttag tt
//
LOCUS       DAEPLF010000284         3501 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEPLF010000284 DAEPLF010000000
VERSION     DAEPLF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.285"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     repeat_region   180..1395
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=379..407
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            2042..3313
                     /locus_tag="KR314_003389"
     CDS             2042..3313
                     /locus_tag="KR314_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0329744.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSIADTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPM
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1394 a          465 c          434 g         1208 t
ORIGIN      
        1 atatatattt ataaactttt caatactttt tataattatt ctacttttat attttaatca
       61 ttaaaactaa agtcaatgtt atctggtaat tatttactac aaatgataaa aaataattat
      121 attttaaagt agaaaatgct gtataaatta ttggggtatt ttacctatac ttgttgcaaa
      181 tttacatacc acgtagttaa tataaaacaa atttcaagcg attggacaac gaataaaaat
      241 tgcaatttat atatcatgta gttaatataa agcatatttg tgtcgatata tagttctaac
      301 taagtataag atttacatac ctcatagtta atataaaaca atgtgtccga attggacaca
      361 tagaaaggaa aagtaaaaat ttacatacca catagttaat ataaaacatt atgataagtt
      421 aaagcatttt acaaatactg atgaatttac ataccacata gttaatataa aactatgtaa
      481 gtcatttagt gttgcagggt ctggtaagat ttacatacca catagttaat ataaaactct
      541 attacaacaa tagtaaagtc tataacaaga ttatatttac ataccacata gttaatataa
      601 aacgaaaaga agacctagat aaaagaatat gcgatataga tttacatacc acatagttaa
      661 tataaaacta tgcttatata ttatgtttat tctgttgttt ctaaaattta cataccacat
      721 agttaatata aaacgatttt ttcatgcgga ggaaaattga tgtggggaca tatttacata
      781 ccacatagtt aatataaaac agataatcta ggcacacata caatgcgcaa aactttattt
      841 acataccaca tagttaatat aaaacattca gacgatatag gcgcaggagc taagtattct
      901 gaaatttaca taccacatag ttaatataaa accagcacgc caagtataag taaaacttcc
      961 actatcacca tttacatacc acatagttaa tataaaacta aatgttgctc cgtaacctaa
     1021 aaacataatg ctccaattta cataccacat agttaatata aaacgtgaaa gaaaaattaa
     1081 tatgctattt acattttaca aatttacata ccacatagtt aatataaaac cattccacaa
     1141 tttatacaat tttttctgtt ctaaaacatt tacataccac atagttaata taaaactctt
     1201 cttatcttcc agttgcaaaa tctatgcttg acctatttac ataccacata gttaatataa
     1261 aactagtttg agtggtaaaa gtacgaagta cccctcttta atttacatac cacatagtta
     1321 atataaaaca tatgtaattc accgttttaa attatagata aattaaattt acataccaca
     1381 tagttaatat aaaaccccaa aataaactaa gtattttcag tacttacaca catacaactc
     1441 tctcaaattt gcagtgaacc atgagtagtg caattgataa catttatcat acaccctcaa
     1501 tcccttacat tgcaactgtt aaaccctact ttatcaaaaa tatcgctcac tgcaaaattt
     1561 atatattttt attatatcat aaaattcaaa ctataaaatt aaaatggaat aatttatcaa
     1621 tttaagaaca cctacatatt tgtaaatact tactatatta tctattctaa taattcttct
     1681 tctagctgga actaatataa aaatcatctc tgaaagatta ggacatacta atataaaaat
     1741 aactatgaat agatattttc ttgttttaaa cgatatggat aaaaaagctt ttgaaaatat
     1801 aatcaaagtt ctctttaaat aacattccat tttatgtaag taagcattat atttttctta
     1861 aatgtcactt aagtgtcagt tatatataat aatagtaaac aactgtattt cactaatatt
     1921 tgctattatc taatagtatt tatagtttag atttgatata atacatgtgg taaaattttg
     1981 atttgtaaaa caaaatttta cctacatata ttaaaacaaa tttaaaacta aggagaccta
     2041 tatgttaaaa gtattaaaag gaaatataat ttttacaaaa acttcagaaa ctttcacagt
     2101 atttgaaaat agttatatta tactggaaaa aggtaaagta aagggaatat ttaaagagat
     2161 acctaaggaa tacaaaaacc taaaagtagt agattacact gataagctta taatacctgg
     2221 tatgaatgac ctccatgccc atgcctctca atttaaaaat cttggaatgg ctatggataa
     2281 agagcttttg ccatggcttg aaacttatac atttccagaa gagtcaaatt ataaagatat
     2341 agaatatgca acaaaaatgt ataaaaaatt tgtaaaagaa ctttggaaat ctggaactac
     2401 aagaatagca gtgtttgcaa cagtacataa agaagcctct atgaaactta tggatatatt
     2461 caaagaagtt ggtcttggag catacgttgg taaagtaaat atgaatatga attgccctga
     2521 tactttatta gaaaacactc aggattctat tgctgataca gaagaaatta taaataagta
     2581 tagtgataca gattctattg taaaaccaat aataacacct agatttatac caagctgtac
     2641 aagtgaactt ttaaaaggtt taggagattt atgtttaaaa tataatattc ctatgcaatc
     2701 acacttatct gaaaattatg atgagattga atgggttaga agtctagaac ctgaatctaa
     2761 attctatgga gatgcatata atagatataa tttatttggt caaactccta ctttaatggc
     2821 tcattgtgtt cattgttctc aggatgaaat agatttaatg agagaaaaca atgttatggc
     2881 agttcactgt ccagcttcta actttaatgt tggtagtggt gctatgccta tcagaaaatt
     2941 aatcgataat aatattagat tagcacttgg atcagatata agcggtggac atacattgtc
     3001 aatatttaaa gcaatggtat ctgcaataca attatctaaa ttatattggg ttaattctgg
     3061 taagaagtat aatttcttat ctttatctga ggctttttat attgcaacca aaagtggtgg
     3121 aagtttcttt ggaaaagttg gtagctttga agaaggttat gattttgatg cattgattat
     3181 tgatgattca aatttaaatc atgacaatta ttctatactt gaaagacttg aaagatttat
     3241 ctatgttggt gatgatagaa atatcattca tagatatgta tgtggaaaac taattgaaga
     3301 acctaatata taattataat aaaaatttta attatatatt atggttaatt tatgcaacta
     3361 taattaataa aagagtatat tttaaatcta aaagctatag gttgaaacat actcttttta
     3421 ttttgtttat ttatagcttt agttattaat catatataaa tctacaaaac gtattcctag
     3481 taacttatat tttttgatat c
//
LOCUS       DAEPLF010000285         3464 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEPLF010000285 DAEPLF010000000
VERSION     DAEPLF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.286"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(67..639)
                     /locus_tag="KR314_003390"
     CDS             complement(67..639)
                     /locus_tag="KR314_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH0329745.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(668..934)
                     /locus_tag="KR314_003391"
     CDS             complement(668..934)
                     /locus_tag="KR314_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBH0329746.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            complement(934..1143)
                     /locus_tag="KR314_003392"
     CDS             complement(934..1143)
                     /locus_tag="KR314_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBH0329747.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            1422..2282
                     /locus_tag="KR314_003393"
     CDS             1422..2282
                     /locus_tag="KR314_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBH0329748.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKSI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2330..3358
                     /locus_tag="KR314_003394"
     CDS             2330..3358
                     /locus_tag="KR314_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBH0329749.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDINKKMDTIDKLKDI
                     EEGYFYLLEDVYLDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
BASE COUNT         1365 a          386 c          549 g         1164 t
ORIGIN      
        1 ttataaattt taacacaaca gtaaattata aattatcaaa taataatagt tatacaaata
       61 aaaattttag aacatttttt gagattcttc taactcgtaa gcatccatta aagcttcacc
      121 aaatctttga tagtgaatta tttctctttg acctaagaat tttaatacat ctataacatc
      181 aacgtcatct gttaggttta taagttgata ataagttgca agtgcttttt gttcagctgc
      241 catatcctca tgtaagtcgg ttacgggatt tgacataact gctataggtc ttacatcaaa
      301 tggaacacca tttgaatctg ttggataaat tccatatcca ttttgagcat agtttgaacc
      361 aagacctgca gcttttaact cttctgggct tgcatcagaa gttaattgat aaaccatagt
      421 acatataagc tcaacgtgag ctagctcttc tgtaccaata tctgttaaaa gtgcacgcat
      481 attaccagta ggcattgtat atctttggct taaatatctt aaagatgcag caagttctcc
      541 atttggccca ccaaattgag ttataagaaa tttagccatt ctagggtcac aagtttttat
      601 attaactggg tgttgtatag ttttctgata tatccacatg aattttcaat cctccaatac
      661 ttgatgtcta ataattaaat tctctgtccc aaggccaagg ttggtcagcc cattgccaag
      721 ggcatctact tggagcatac ccaaagttag ttaaaggacc gtatttattt tcgtaagcat
      781 atctagcttg cgcaaattga ttacataatg aattataagt gttgatagca tttttatcat
      841 cagggtgatt gtctaaatat aagttcatat caacacatgc aaaagatgtt tcctgaactt
      901 ttctcataag ttcagctcta gatgagtctt tcattatctt ctacctcctc tatatttttt
      961 tggcttagca tataattctt tgttgtacat ttcagactct agtaagttta gttctggaaa
     1021 tatagttcca aattttaatg catcagaaag gcagtacatt tctgtgaaaa tttgattatt
     1081 aatgtatggt ctagccaaag atggaccaca attttttttg atatctctag tcttattttc
     1141 caagatgaaa acctccttgt tgtaaataga taggtaactc tatctagtgt ataatattca
     1201 atcatttaaa aatcgttact aataataaaa tattaaatga gaaaaataaa aaagataata
     1261 aaagaaagag gttatttatg ttattataag tttatgtgtt attgtaaact ataaattatt
     1321 tagtaaaatt aaagtaaaaa ttatagtatt ttgtggatga aggtagactt ttttgtaaaa
     1381 tattattaac ataaatatac ataaatatac gaggtgatta gatgagtgaa ctatcaggaa
     1441 aaggctgtga aattatagtt ccttttgagg aaagacttcc agtaagagat atagaaaaaa
     1501 gtataataaa gaaatataga aaaaatttat ggtctaagtt tatgaagtca ataagagatt
     1561 ataaattggt tgaagaaggg gataagatag ctgtagcgat ttctggtgga aaagacagta
     1621 ttcttatggc taaaatgttt caagagttaa aaaaacatgg acaagttaat tttgatgttg
     1681 aatttattgc tatggaccct ggataccatg caaatataag acaattactt atagataatt
     1741 gtgagtatct aaatatacca atacatttat ttgattcaag aatatttgag atagcagatg
     1801 aaatagcaaa agattatcca tgctatatgt gtgcaagaat gaggagagga gcattatatt
     1861 caaaggcaga agaattaggg tgtaacaaac ttgcattagg tcatcattat gatgatgtaa
     1921 tagagacaac aatgcttaac ttattgtgtg ctggaaattt taaaacaatg cttcctaaac
     1981 ttaattctac taattttgaa ggtataaaaa ttataagacc actttattat ataagagaag
     2041 aacatattat aagatttatt caaaatagtg gtatatggcc attaaattgt gcatgtatgg
     2101 ttgctgctaa aaaaacaggt aataaaagat atgagataaa agacttaata aagagcttag
     2161 aatcaaattt taaaaatgta gaaaaatcta tatttaaggc agcagaaaat gtaaatcttg
     2221 attcagtact tggttggcaa aaagatgggg agaaacattc tttcttagaa aattttgaat
     2281 aaaaatagtt aaaaagaatc actataaaca attggaaagg gtgtttatat tgaaaagagt
     2341 atatatagat tttgaaatga atatgccaaa ttctaaatca aaaagagcag tatttaattc
     2401 tgatataatt gcaatcggag cagttatgta tgatgaaaaa acaaaaaata ttgataaatt
     2461 taaatcttta ataagaccag taagtaatga ggagttatat cctcatatac aagaattaac
     2521 acatatatct tctgaagaat tgaagagtgc accaagttat gaagaggtca tgagaaaatt
     2581 taaaaaatgg cttggcatat tttctgatat taaaggaata tatacttttg gtaatttgga
     2641 tttgacatgt ttcaataaca cagatatgag aagtgctaaa aaaaataacc atccaaggtt
     2701 tgtaaataat attagagatt tatttgtaga tataaaagaa aaatatataa attgtggtat
     2761 gagatgtatg aactatatat cattgaaaaa tttacttgaa tttgttaact tagagtttag
     2821 tggagatgcc catgacccat taaatgatgc atataattta tttattttgg atgaggcaat
     2881 cacaaataat gtagatatac aaaatctttt aataatacgt gacataataa gaccaccatt
     2941 taacgatata aattctaatt tagataattg ttttaacaaa tttaaggagt cattatataa
     3001 aaaagaagga aattataata ttgtagattt ttctgttgaa ataataaaaa ctgtgagaat
     3061 gtacttatta acaatcatag atgtaaatat acaaaattta gaaattatga aagatataaa
     3121 taaaaaaatg gatactatag ataaattgaa agatatagag gaaggctatt tttatttatt
     3181 agaagatgtg tacctcgaca tgaaagatgt attagaagat ttaatgcttt atagaatgaa
     3241 tgaagatgag tacaagtatg agataaaaaa tataataaaa atgtttgatg aagacttaga
     3301 taatgaaaaa atatatataa ataaatgtaa taacttaaat gttgttaaca agatataaga
     3361 aattacaaat aatagagtag aacttgacaa ttataacaat tttttataaa actgaatcaa
     3421 gataaaatta aattaatata aaaataatat ataataaaaa atgt
//
LOCUS       DAEPLF010000286         3437 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEPLF010000286 DAEPLF010000000
VERSION     DAEPLF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.287"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..256
                     /locus_tag="KR314_003395"
     CDS             <1..256
                     /locus_tag="KR314_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBH0329750.1"
                     /translation="LFLLLQFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLI
                     RENVLVLLLVICLSTLIVMYTTGIIVEKMIKKKEEGQKNV"
     gene            249..938
                     /locus_tag="KR314_003396"
     CDS             249..938
                     /locus_tag="KR314_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBH0329751.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            1458..2324
                     /locus_tag="KR314_003397"
     CDS             1458..2324
                     /locus_tag="KR314_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0329752.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            2566..3375
                     /locus_tag="KR314_003398"
     CDS             2566..3375
                     /locus_tag="KR314_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329753.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKKGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGQGSYTFFVDEGT
                     AMEYIGEFKEDKKNGKGNFVFSNGNEYIGHFEEDMMNGIGNMLYNSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
BASE COUNT         1396 a          317 c          603 g         1121 t
ORIGIN      
        1 attatttcta ctactacaat ttaaagtttt agacttgagt agtatagaaa atgtaagtgg
       61 atttttcctc gataatatgg ctatattttt tattccagca ggtgtatcac ttataaagtc
      121 attagaccta ataagggaaa atgtattagt gcttttatta gtcatatgtt taagtacact
      181 aatagtaatg tatactactg gaattattgt ggagaaaatg ataaaaaaga aggaagaagg
      241 acaaaaaaat gtataatgta ttacagacac cagtatttgg tataatagta acaatagttt
      301 ttttcaattt aggaagatac attcaacgaa aaacaagtaa tcctatatgt aaccctttat
      361 taattgcaat cgtaggaata atattgtttt taagcatatc taaaatccct tatgagaatt
      421 acaagatagg agctgatagt ctaaatttct ttttaggtcc tgtcacagta gtacttgcag
      481 ttccacttta taaacaattt gaattattta aaaagcatat gtttgagatt attgtaggaa
      541 taggttgtgg tatagtgatt tcttttgtat ctgtattgat aattggcaaa atagctaatt
      601 cagatgtaag tataataaat tcactaattc caaaatctat aacaacacca atgggaatat
      661 ctttagcaaa ttctttaaat ggtatagaat caataacagt tgtagccata atttttacag
      721 gtatttttgg tggtatggtt gcttctacag tattcaaact ggggaatata aatcatccag
      781 ttgctaaagg aattgctctt ggaacatctg cacatgcttt aggaactaca aaagcttttg
      841 aacttggaga aatagaagga gctatgagtg gtgtttctat tggagtttct ggtacaatta
      901 cagtaatact aattccaatt ataatgaatt ttatataaaa aataaaatta agacgagtac
      961 cttgaatact taaaacaaaa taaaataaaa aaaatttaaa ataaaaactg aacgaaaaaa
     1021 atgcaaataa tgaaataaaa aaatcatgta ctgaaattat aaaaagaaaa atttcattta
     1081 ggaattaatt ataaagagat aattactaaa cttgaatttg ctaaaataaa ataaattact
     1141 gcgaaaatag atatttagaa aaaaatacta taaatttact atattgcaag ttaaataata
     1201 agcagtatca tattatgcta gaaaaagttt catttaaggc ataaatgaga tgatttgaac
     1261 gtttttaaag attagaatat aaaagattgc actagacata tatgaaaaag ataatataat
     1321 agatgatgta atagatgaca gttcaagaaa ttataaaaaa aatttatttg aaatattatc
     1381 tattacatca ggaataatat atgtaagagt aagcttttaa aattctaaag atttagaaag
     1441 attgaaggga gaaagaaatg agtgaagtta agaataccct acaaaaacat ccagttttaa
     1501 gatattcaac aatgctattt gggatgttga tgacatctat aggaataaat gggttcttaa
     1561 gaccagcaca tctgcttagt ggaggtgcta caggtatagc aacatctata aactatttga
     1621 caaatataaa tgtaggttta ttaacatttt taatcaatat tccaatattt atcttagggt
     1681 tcatatacct tgaaaaagag ttttgtattt caagtttggt aaatatgatt gtattttcat
     1741 tattattagg agcaactcag gaaataagta atattatacc tatacatgat atattgcttc
     1801 aaagtgtata tggtggtata ttatctggtt taggagtcgg agttgtattt agaactaggt
     1861 cttctcaagg tggtacagat ataatagctg caatactaaa aattaagaag aatatagaaa
     1921 tgaaagacac tgctttagca ataaatggct tgatagtact tacaggaagt tttttatttg
     1981 ggttggattt agcattatac actcttatag gtctatttct aaatgcgtat tcaatgagtt
     2041 ttattaaaga tgcaatgaat taccaaaaat ctgttatggt aatgtcaaat gaagtcgatt
     2101 taatagcaga agatataatg aagagtttag ttagaggtgt aactttttta gatgcagaag
     2161 gtgcatacac tcatcaaaaa aagaagatta tatatacaat agtttcatca aatgagatac
     2221 caaagataaa agatatagct ttgaagtatg ataaaaaggc ctttatatct gtaaatgatg
     2281 ttacagaagt aaaaggaaga gggtttaaag caaaagattt atagaaaatt ttacaagctg
     2341 ggagtttata atgtaaattt gtatattata tctctcagct tatttaaatt tagtatatga
     2401 aaataaaggt tttatcaatg atttgtgtat cactttaaaa gatatcattt taaaattaaa
     2461 attgaaaaaa ttactcaata tgtagaaaac ataaccacta ttaggatata ataaatttaa
     2521 tagatttagt aaaatatcta ggaataacgt tggaggaata gcattatgaa tttatctaaa
     2581 gaatatgtta atttgtatga taattatttg aaatatataa atgaagtaaa caaagagatt
     2641 agaagtataa agaacatgaa gaaacgtgtt ttaaaaaagg gcgattttgc tccagaaaat
     2701 gaagtattag aattggaagg aaaaacaata cgcgatatag atgatgtcga tacaaaaaaa
     2761 ccaaaaaata aaatatataa attttcaaat ggggatgtgt atgtaggaaa atttttagaa
     2821 ggaaagatgg aagggcaagg atcatatact ttttttgttg atgaaggaac ggctatggag
     2881 tacataggag agtttaaaga agacaaaaaa aacggaaaag gaaactttgt attttcaaat
     2941 ggaaatgaat atataggcca ttttgaggaa gatatgatga acggtattgg aaatatgtta
     3001 tataatagca aagatgaata tattggaact tggaaaaatg gaaaaaaaga tggaaaaggt
     3061 atttataagt ggagtgatgg atgtatatat gcaggtgaat ttaaaggtgg aaagatggaa
     3121 gggtatggta tttgctataa tagtaaaggg gaagtacttt atgaaggaga atggaagaat
     3181 aatttgattc atggtaaagg aacttatata tgggaaaaag gtaaaaagta tataggtgaa
     3241 tttatgcatg gtaaaaaaca tggacaagga actttttatc taaataatga attggtttat
     3301 gaagggactt ggaaatttga taagccatcc atcttcgaca gaacattaga tgaaatattt
     3361 tcattaagac tatagcttta tctattaata gataaagctt ttttctaata cttactgtaa
     3421 ataagtatga atgatat
//
LOCUS       DAEPLF010000287         3427 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEPLF010000287 DAEPLF010000000
VERSION     DAEPLF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.288"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(74..1666)
                     /gene="hcp"
                     /locus_tag="KR314_003399"
                     /gene_synonym="priS"
     CDS             complement(74..1666)
                     /gene="hcp"
                     /locus_tag="KR314_003399"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBH0329754.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKLG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(1726..2451)
                     /locus_tag="KR314_003400"
     CDS             complement(1726..2451)
                     /locus_tag="KR314_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0329755.1"
                     /translation="MMRKIIKIDEGKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            2592..3320
                     /locus_tag="KR314_003401"
     CDS             2592..3320
                     /locus_tag="KR314_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBH0329756.1"
                     /translation="MKTNYRLHMQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEM
                     LECLGVVDKTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLF
                     GEVFACTRLHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKN
                     ILLHNKIELLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRE
                     LGKMRDEGLLSFNKNKFEIFYFQK"
BASE COUNT         1095 a          615 c          424 g         1293 t
ORIGIN      
        1 aaccccttat cttaattgtg acatacttaa aaatatttta actataaata tctttaagta
       61 tagatatatt tatttatcct aaaatcttct tcatatcttc ttcaggagta gatataggtg
      121 tcaatgcaaa tttatcaacc aatatatcta atacattctt agataagaat gctggcaaag
      181 aaggccctat atagatattt ttgataccta aatgaagtag tgtaagaagt atacaaacag
      241 ctttctgctc ataccatgac agtaccatgc taagaggcaa atcatttact ccacattcaa
      301 aagcatttgc caatgaaact gccactttaa ttgcactata agcatcatta cattgtccca
      361 tatccattaa acgaggtaag ccacctatat ccccaaggtc taaatcatta aatctatatt
      421 ttccacatgc aagtgtcaat ataacagtat ctttaggagc ttgttttaca aattcagtgt
      481 aataatttct tccaagtttt gcaccatcac aacctccaac caataaaaag tgtcttatag
      541 ctccactctt tacagcatct actactttat cttgtacaga tgaaactgta cctcttgcaa
      601 aaccagtagt taatgtatct cctccattaa taccagtcat atgaacatct tctctatatc
      661 ctccaagttc aagagctttt tctattacac tagaaaaatc tttatcttca tcaatatgaa
      721 ccatacctgg ataagcaact acttctgtag taaatactct atctctataa ctatctttaa
      781 caggcattaa acagtttgtt gtaaataata ttggagcagg aatattatca aattcttttt
      841 gttggttttg ccatgctgta ccaaaattac cttttagatg tttatattct tttagttttg
      901 gatatgcatg tgctggtaat aactcaccat gagtatatat atttataccc ttatctttcg
      961 tttgttctaa taatttgtgt aaatcataaa ggtcatgtcc tgtaactaca ataaatggcc
     1021 ctttctcgat tttcatagat acctgagtcg gttctggtgt tccataagtt tcagtatttg
     1081 ctctatcaag caattccata cattttaagt ttatttctcc agttttcata actagaccta
     1141 aaagttcgtc aatagacaag tctcctccaa ctgctattaa tccttcatag aaaaattcat
     1201 ttacgtcttt gtctgtataa ccaagtacca ttgcatgata tgcataggca gccatgcctc
     1261 ttaatccaaa taagattaat gattttaatg aacgtatatc ctcattatca ttccataaat
     1321 ccttcatatc atagttatca tttctattac aagttgttga acaatctgaa catctaggta
     1381 caagtttact tttttctacc tcagtttttt caatttgtct ttttatagct tcatcatcaa
     1441 aacttacatt cgttatagta gtgaatagcc cctctatcat aattttatct gtttcacttg
     1501 ttggtctatt cttatttcca atagttgctc ttgaaagacc aatcaacact cctgtaagct
     1561 cgtcttgcaa gttagctgta cccccctttt taccacaaac accatttata gtacaacctt
     1621 tacctcctgc tgtttgttca cattgaaaac aaaacatttt ttccattttt tattcctccc
     1681 tttttattta ttaaaattta ttatattttt ctatgattca cttaattaaa tttctccatc
     1741 tacaattttt ccatctacag atatagtaac tatttgccat ggtatcattt ttccactatt
     1801 ttgtagagct gtcttaacag catttactat accaccacag caaggaactt ccatacgtgt
     1861 tactactata tttttaatat cattttcttt taaaatctct gtcagttttt cagcatagtc
     1921 aacttcatca agtttagggc atccgattaa tgtaacctta tttttcataa atttattatg
     1981 aaaatttcca tatgcatacg ctgtacagtc tgctgctatt aaaagacttg cattctttaa
     2041 atatgatgca ttagttggta ctaattttat ttgtactggc cattgattaa gttgagaacc
     2101 ttttatctct actatatcat tgcttataga tgtactattt gaaacctctc tatttattga
     2161 cttagactga gaacctggac atccacatgc taaagttttc ttttgtgctt tctttgcttc
     2221 catatttgct tttactgcag cttcatcata ttctgctgct tctctatact caaatgaaat
     2281 cgcatttgtt ggacaagttg gcagacaatc tcccagccca tcacaatagt catctcttaa
     2341 aagttttgct tttccattta ccattcctat agcttcttca tgacaagctt caacacatag
     2401 tccacaacca ttacactttc cctcatcaat tttaattatt tttctcatca tttatatcac
     2461 ctcataaaat ttttttaatt taaagtattg atttcttata tatttatttt taatacctat
     2521 atttctttat ttcaaatttc ttgtagtagg atttttatat ccccttgctt ttataaactt
     2581 agtatagtac aatgaaaaca aattatcggt tgcatatgca acaaggaagt gattatttga
     2641 aaaaattttt agaaaagcat atagacctac ttaaaaaaat tcctctattt gaaggaatta
     2701 aaagtgatga gcttgaagaa atgttggaat gtttaggtgt tgtagataag acttacccaa
     2761 aagatagtat aatattctct gttggaagtg aagttacttc tattggaatt atgcttaaag
     2821 gaagtgctca tataataaag gaagatatcg aagggaatag gaatattgta gctgaactct
     2881 tgcctggaga tttatttgga gaggtattcg catgtactag actacataaa agtaatgtta
     2941 cagtaaccac aacatcttct tgcgaagttt tatttataaa gtttaaaagt gtaactggta
     3001 tttgttcatc tgcatgtgta tttcataata ggcttgttga aaatatgcta caattaatag
     3061 ctgaaaaaaa cattttactt cacaataaaa tagaattact ttctcgtaaa acaactcgcg
     3121 agaaattgct tatgtatttt tcaaaacaga tagaacaaac aggtagccac cagtttacaa
     3181 ttcccttttc aagaaatgaa atggctgatt ttttatgtgt tgacagaagt tctatgtcaa
     3241 gagagcttgg caagatgcga gatgaaggct tgttatcttt taataaaaat aaatttgaaa
     3301 tcttttactt tcaaaaataa actagatatt taaacatatt tatttcaata tatactatat
     3361 tagttaattc aaattataaa tatcatattt gaatcttaaa tttatatttt tacttaaaaa
     3421 gttaata
//
LOCUS       DAEPLF010000288         3419 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEPLF010000288 DAEPLF010000000
VERSION     DAEPLF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.289"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            262..807
                     /locus_tag="KR314_003402"
     CDS             262..807
                     /locus_tag="KR314_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBH0329757.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            820..1833
                     /locus_tag="KR314_003403"
     CDS             820..1833
                     /locus_tag="KR314_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBH0329758.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            2055..>3419
                     /locus_tag="KR314_003404"
     CDS             2055..>3419
                     /locus_tag="KR314_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0329759.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKENNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLD"
BASE COUNT         1338 a          335 c          631 g         1115 t
ORIGIN      
        1 gttcttaaat ttctaagaag aatttagaat atagtcgaaa taagaattgt atatttctga
       61 aaattctaat tagtacaatt ttttccttta taaaattgaa aaaagatgta tttatttatc
      121 aaaaatatta tattaaaatg atattaataa aaaaacattt acaatattag catatagtac
      181 agaaaaaaga tgtaatttgt aaaatttatg ttaagcagta aaattaaaaa tttaaagata
      241 ttatagggag gttataattt tatggaaaac gttattacag gtctaagtaa aggtgcagag
      301 tggtttatcg gtttgtttca gaaaggggga gaacaattta taggtcttgt ttcaggtact
      361 cttccaacgc taattgtatt aatggtagct attaactctt tgattaagat aattggtgag
      421 gaaagagtta ataattgggc ttctaagcta ggaaagaact cttttacaag atatattcta
      481 cttccattag tatcagtatt tttcttggga aatcctatgt gctatacatt tggtagattt
      541 ttaaaagaag agcacaaagc aggattttat gatgcagcag tttcatttgt acatccaatt
      601 acaggtcttt ttccacatgc aaatgcagga gaactttttg tatggttggg aatatcagca
      661 ggtttgacta ctttaggtaa agaaactact acactggcat tgtggtattt tatagttggt
      721 ctaattgtaa tttttataag aggtatagta acagaaaaaa tgtatgcttt tctaacaaga
      781 aaaaatagag caacaaataa ggcttaggag gtagataaaa tggaggagaa taaaatatta
      841 aaaatagaaa agggaacttc aggctgggga ggtcctttgt atataaaaaa agaagggaat
      901 agaaataaga tactatcaat gactgctggt ggtatccatg aagtaacatt aaaaataaaa
      961 gaactactgg gttgtgaaat tgtagatgga tttaaaactg gagtttctga tgaagaagta
     1021 gcagtagtta ttattgattg tggtggaact gctagatgtg gagtttatcc aaagaaaaaa
     1081 atacctacaa taaatgtaaa tccagttgga aaaacaggtc ctttagcaaa atttataaca
     1141 gaagaatatt atgtatcaga tgttaatcct aactgtataa gtgttgtaga tggggaagat
     1201 atgccacaaa aatctcaaga aaataaaagc gaaaataaga gttctattag aaagcctgat
     1261 aattatgatg aggttaagtc aaaagcacaa ggtgaatatg ctaaaaagaa cataatactt
     1321 tctattggtc aaggggcagg gcaagtagta tcaaaatttt atgatgcagg tcgagacact
     1381 atccaaatgg taatgaataa cgtaatacca tttatggcat ttgtaagtat gcttatggga
     1441 attattttag catctggact gggagactgg attgcaagag ttatatctcc acttgcaggt
     1501 aatattggtg gattattgat tatatcagta atttgcacac ttccattctt atcaccaata
     1561 ctaggacctg gtgcagttat agctcaagta gtagggactc ttgtaggtac acaaattggt
     1621 cttggtgcaa taccagcata tttagcactt ccagctttat ttgcaataaa tgggcaagct
     1681 ggatgtgatt ttgtaccagt aggattaagt ttgggagaag cagaaccaga aactgtagag
     1741 tatggtgttc cagctttatt ttactctcgt ttaataactg gtccaatagc tgttattatt
     1801 gcatatggag ttgcagtatt tgcattgaga taatatttaa tgtcaatata aggcttggaa
     1861 ttaattaata aaattgtaaa tataaattta agctagtcaa gattttttgt taatgaaaga
     1921 ctagctttta tactattaat tagctgtaat tatcgagtta gattaatact aaattttcct
     1981 ttattaaagt gaaattttat gtattaattt ggtttcaaat ttgtactaga atgaaatcaa
     2041 ggaggagata caaaatgagt gtaactgaga aaattccttc tattgaccgc aagatattta
     2101 agatattgat tatgtgtagt aaaaaagagt ttgtgagtat taattcaata gcaaatgaat
     2161 tgaatgtcac aactagaagt gtaaggacgt atataaaaca attgaataaa gatttaggta
     2221 atgatatagc agtaatcaaa tatataaagg gtcaaggcta taaactagaa ataaaagatg
     2281 agcagatact caataagatt attgatatga atagaaaaaa tgtattttca ttaaattcaa
     2341 aagaagatag agtagagttt atactaaatt acttgataga actagatgga tttattacac
     2401 ttgatagttt agctgatgag atgtgtgtag gaagaactac tttagtaaat gatttccagt
     2461 atgtagaaaa agttttggct tcatataatt taaacttgat taaaaaacag aatacaggta
     2521 tgaaattaaa tggaaatgaa cttgatatta gactgtttat attaaatcag ttgtataaaa
     2581 actcaaggaa agatttcagt aattcaaagt attttaaagg aatcaaaaaa gaagaaatca
     2641 taaatttaga agaaaaacta ttgactttat ttaaagaaaa taatttttat gttacagatg
     2701 aaatgttaag agaagttatt aactacatag tagttttagt ctatagagta aaagaagaaa
     2761 agaaagtaaa agactatgat gtaaagtttg atttacttaa atcatatgat gaatacttta
     2821 ttgctaggga aataaaacat ataatatcag gaatgtttga atgtacttta aatgatgaag
     2881 aaattattta tttaactata ccattagtta gtggaaatgc accagcatca gaatgtgctt
     2941 taaatagtag tagaattagt aaaaacattg atgagttgat ggaaaatata tttaatcaaa
     3001 tatatgtcga tatgggtatt tttataaatg aggatgaatt aagagtaggt ctaggatatc
     3061 atcttagttt tactttaaac aggctattat ttaatataaa gttaaaaaat gttttgttag
     3121 aagagattaa gcaaaattat atacttccat ttaaattagc acaaatagca ggaaaagtaa
     3181 tagagaaaaa gtataactta gaagtttcag aagatgagat tggatatata gcaatccatt
     3241 tcagtggata tttagaaaga aatagcagta gattctattc aataaaaaaa atagcaatta
     3301 tatgtagtac aggtctagga acagcaaaac tattgaaaat aagagtagaa aaactgatag
     3361 gaaataatcc taaaattgat actctttcaa gttttaattt gagaaatatt aacttagat
//
LOCUS       DAEPLF010000289         3412 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEPLF010000289 DAEPLF010000000
VERSION     DAEPLF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.290"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..281)
                     /locus_tag="KR314_003405"
     CDS             complement(<1..281)
                     /locus_tag="KR314_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBH0329760.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLL"
     gene            complement(429..1220)
                     /locus_tag="KR314_003406"
     CDS             complement(429..1220)
                     /locus_tag="KR314_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0329761.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(1256..1912)
                     /locus_tag="KR314_003407"
     CDS             complement(1256..1912)
                     /locus_tag="KR314_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329762.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(1905..2870)
                     /locus_tag="KR314_003408"
     CDS             complement(1905..2870)
                     /locus_tag="KR314_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBH0329763.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEHVKQYLTSKGIRWEEMINE"
     regulatory      complement(2976..3082)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         1065 a          604 c          344 g         1399 t
ORIGIN      
        1 ttaagcaata caatttggaa tttttgaatt tttaaattta ctactcctaa aatacttaca
       61 aaaactaata caagacaccc tactttcaat atatttataa attcatctgt tttaaagcta
      121 cttatataaa ctatagaata tccaaatatc caaagtaaaa ttattctata catatttata
      181 gaaactgtat acaaatttat atttaaaagt gcaataacca aaaataaccc attagctagt
      241 atatagacct tattcttact taaagttcct ttagattcca tattccccac cacttcttca
      301 tttttatata gttttaccta taaatatgtt accacaattc ttccaaaaaa tttgtcactt
      361 tctagttata aaaaaaaatg agtaaatatt tttaatttta ctcattttgt agattttgta
      421 tattcagttt aaaaagctgg aactatactt cctttatatt catcttgtat aaatttttta
      481 acttctttac tattcatagc ctctgataaa gccttaatct tatcactatc tttattattt
      541 tctctacaag ctattatatt tacatatgga gaatcacttg attctattac tattgcatcc
      601 ttagtaggat ttaagttagc tgttaaagca taattagaat ttattactgc tccgtcaaca
      661 tcttttaata ctgttggtaa ttgctctgca ttcatttctt ttattactat atttttagga
      721 tttttagtta tatctttttt agttataagc tctccatctt ttacttctat caatttattt
      781 tttgctaata atttaagtgc tctagctcca ttagttgcat catttggcac agctataaca
      841 gctccatctt ttaattcatc taatgactta actttttctg aatagaatcc cattggctct
      901 atatgaactt tagatgtata agtaagttta tatcctttct ccttcacagt ttcctctaaa
      961 aatggaatat gttgaaagaa gttagcatcc aaagaccctt catctaaagc tgtatttgga
     1021 agtacataat catcaaatat ttttacttct aaatcatatc ctttttcttt taacaaaggt
     1081 tttgcaactt ccaatatctt tgcatgtgga tttgatgaag ctcctactag tattttttta
     1141 tcctctttgt ttgaacaacc tactgctgat attgctattg ctgacaccaa tgctactgat
     1201 aataatttct ttaatttcat ttttactccc cctctttaaa atgtttataa ttaatttatt
     1261 ttagtttctt ataagccaga tttcctactc cctggattat ctgaactaat attataagtg
     1321 ctattacagt atataccatt actgctgtat caaatctttg atatccataa ataagtgcta
     1381 tattacctaa tcctccagct cctactgcac ctgccatagc tgtataacca agtatagata
     1441 ttattgataa tgtaattcca gatactattg aaggcatagc ttcctttatc ataactttaa
     1501 atactatttg tctttttgta gctccaaaag attttgctgc ttctatcaat cctttatcaa
     1561 cttcatttaa tgagctttct ataattctag cgataaacgg tgctgcccca atagttattg
     1621 gcactattgc agcagtttcc cctatacttg ttccaacgat taatctagtt attggaatca
     1681 ttgcaactat aagtatcata aatgggaaac ttctaaatat atttactata aatcctaatg
     1741 cactatttat tgtgctattt ggttttaatc catctggttt tgtaactact aatatgattg
     1801 ctaatataaa tcctaatatt gttgccacta tagttggtac tataaccata tacaaagttt
     1861 gtaacaatgc atttggaaat agtgttgtta aaaagtcaat taaactattc atttatcatt
     1921 tcctcccatc ttatgccctt actagttaga tattgtttta cgtgctctcc acttttatct
     1981 gaaatgttta taataagtga acctaaaata tcatctttaa attgttctaa tttcccaaat
     2041 attatagaca catctatatt tagttctctt gccatagtcg taataatagc ttcattagaa
     2101 atatctttag ggaatagtat ctttatattt gttccttttg gcaatattat actttcttca
     2161 cctattaatt ttctaagtcc tttagtattt cttaagaata tttcttctgt atctcctacc
     2221 tctaatactt ctccattttc cattattgct actcttccac aaatctgctt tataacttcc
     2281 atttgatgag ttacaactat tattgttatt cccaattttt tatttatatc ttcaagcaat
     2341 gaaagaattg actttgtagt atttgggtct aatgctgaag ttgcttcatc acaaagaagt
     2401 acttgtgggt ttagtgctaa agctctagca attgcaaccc tttgtttttg tccgccactt
     2461 agatttctgg gtttatcatt tttcttttca gatattccaa ctacttctaa taactctaaa
     2521 actctttttt taatttctgc ctttgagtat ccaaaacatt ctaatggcaa tgctacatta
     2581 tcaaatacag tttttctttc aagcaatgaa aagtgttgaa aaatcattcc taactctttt
     2641 cttaaatctc tcatttgctt ttcattcaat gactttactt ctttatcaga aacaagtaca
     2701 ctgccttctt ggtattcttc taatccgttt atacatctca gtagtgttga tttccctgca
     2761 ccactatgac ctattattcc aaaaatttct ccagattcta tttcaatact tacatcttta
     2821 agcacttgaa tctttccata atacttatta acatttttta tgctaatcat aaaatttcct
     2881 cccatatatt ctgctcagtt tttatgttta tcctctctta tgtattacaa caaaataaaa
     2941 gcccgagtat aaactcaggc ttagaaagct atttatactc atctatcaac tatattagtt
     3001 gcaggattta gcaccaatgt atataaaata tactggttgc cgggtttcaa agggccagtc
     3061 cctccaccac tcttgataag acgttattta attcaattct attatcattt gttgctatgt
     3121 tatatatgat atattcatta atagtatttg tcaataaatt tatttaaatt ttttattttt
     3181 tattatcgtt aattaactat ttttaattag ttttctagct cctaacactt tatattttaa
     3241 tgtctatata actatagaac atcattataa gaaataaatt aaataacatc aatctatcta
     3301 aatcatctgt aaagtaaatt aagaaaatta acattttatc caaactctat cctactatta
     3361 aataaaataa tttaaataca atgatgatta tatagctcta atactttaga ct
//
LOCUS       DAEPLF010000290         3399 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEPLF010000290 DAEPLF010000000
VERSION     DAEPLF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.291"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            55..2166
                     /locus_tag="KR314_003409"
     CDS             55..2166
                     /locus_tag="KR314_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBH0329764.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTAL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(2364..3329)
                     /locus_tag="KR314_003410"
     CDS             complement(2364..3329)
                     /locus_tag="KR314_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBH0329765.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
BASE COUNT         1317 a          463 c          569 g         1050 t
ORIGIN      
        1 ttaataagaa gtatattgta gaaaaaataa atttaaaaaa ttaaggggga tgaaatgaaa
       61 aaaataagta tattagtatt atcattgata atgacactta ctatgtgtag tgtaagttca
      121 tttgccgatt catcaaatga taaggaaatg agagctgctt ggatatctac agtttataat
      181 ttagactggc ctaagactaa gaataatgaa gccaagcaaa aaaaggaata cacagattta
      241 ttggacaaac taaaaagtgt aggaataaat acagcagtgg ttcaagtaag accaaaatca
      301 gatgctttat ataaatcaaa tataaaccca tggtctgaat atcttaccgg tacacaaggt
      361 aaagaccctg gatatgaccc attaccattt ttaatagaag aagcacataa aagaggaatg
      421 gaatttcatg catggtttaa tccatataga ataactatgg cagatgagag catagataaa
      481 ttgccagcaa accatccagc aaagaaaaat ccaagctggg tagttaaaca tggaaataag
      541 tattactacg acccaggttt accagaagtt agaaagtaca tagtggatag tatagcagaa
      601 gtagttcaaa attacgatat agatggagtt cattttgatg attacttcta tccaggagta
      661 agtttcaatg atactgcaac ataccaaaaa tatggaaaag gacaaaataa ggataattgg
      721 agaagagaaa atgtaaatac tctacttaga gatgtaaaag cgtctataaa gagtataaag
      781 cctaatgttg tatttggtgt aagcccagca ggtatatgga gaaataagag tagtgaccca
      841 acaggttcag atacatcagg taatgaaagt tatgtaggta catatgcaga tacaagagca
      901 tggataaaac aaggattgat tgactatgta gttccacaac tttactggcc aattggtctt
      961 aaagcagcag attattctaa attagtggca tggtgggcta atgaagtaaa aggaacaaat
     1021 gtagacttat acataggaca aggtatatat aagcaaggtc aatctagtta tggtggacaa
     1081 aatatagcaa aagaaatagt acaacaagta acattaaata gaaagtatag tgaaataaaa
     1141 ggtagtatgt atttctcagc taaagatata gcaaatagta cttctataca aaaggattta
     1201 aagagtttat attctagtag tgaagaacca gttactccac catctaatgt taaagtggag
     1261 aaattaagag gcgacgaaag atatgacaca gcagtagcaa taagtaaaaa aggttgggct
     1321 acaaattcag atacagtagt attagtaaat gggtactcta tagttgatgg cataacatca
     1381 acaccacttg ctacatcaaa tgatgctcct atactattgg taaataagga taatatacct
     1441 acatccacta agaatgagtt aaaaagactt aatccaagta aagttatttt aattggagga
     1501 aataactcta ttggagataa agttgagagt gagataaaag atacattgtc aaatgtatca
     1561 ataaatagag ttggtggttc agatagatat agtacatctt taatgatagc aaaagaatta
     1621 gtaaaaacta atccagtaga aaaactatat ataacaagtg gtacaggtga agcagattct
     1681 ctttctatag cttcaaaagc aggagaagaa aagcaaccaa tagtactagt atcaaaagat
     1741 aatgtaagtg atgaagttta caattggata tcagatttaa aagtaaaaga tgcttatttt
     1801 ataggtggta atctaagtat atctgattca gtaataaata agctggataa ggtcattaca
     1861 aatgatgtat ctaaaaatag aatagctgga gaaaatagag aagaaacaaa tggaaaagta
     1921 atacaaaaat tctatccaaa tgctgaatat tcatcaatgt ttgtaagtaa aagtaatcaa
     1981 ttggtagatg cattaacatc aggtccttta gcagctaaat taaaatcacc agtagttatg
     2041 cttggaaata gtgtaacatc tgctcagaaa actgctttag agcctaagaa aactgctcta
     2101 gtatacgaag ctggagatgg tataaatcaa aacacattaa acactttctt aaacttagtt
     2161 aagtaataaa aaagttaatt taaaatacag agtattttat aaatgtagat taaaatgaaa
     2221 attatcatca taaattagaa aataaaaaag aactatatgc attttgtaat caagtgtaca
     2281 cagcaacttg aaaataatta tgcgatatag ttctttttta gtaattaatc ttatactttt
     2341 taataaactt tatgttgaat tttttattgt ttaaactcta tgtcattaaa tataaaatcg
     2401 tgtaatatgt ctaaagtatc tgggtcaaat ctaagaaccc atccagcttt tccatatata
     2461 ccacctgtac tatgaatttc atcatcaata ggaaattcaa attgcttaat atttaagtca
     2521 cccattttta aaacttgagc tcctaaagaa agtattgcat taggcttcat gttagtttta
     2581 acatatggaa gaactgcatc taataaacct ggatatttag taacaggtaa agtttccgtt
     2641 ttcttgatta tagcttgtat aatctgtctc tgtctgccat ctctagcaaa ggctgtatcg
     2701 ttgtgtctga tacgtgcaaa agatagtgcc tgatatccat ttagagtctg ctttccagag
     2761 tttctaatgt attgtatgct acctttatca tcacttttat tccatttata agtttcagga
     2821 ataaatttat ttagttctcg tatttctcct ttttcaacag taacctcaac gccaccaata
     2881 gcatcaatta tatacataaa tgattcgaaa ttaacacaag cataattttg aatatcaata
     2941 ttaaaattct cttcaattgt ttgtattaat aaatcagctt gtccataagc ataagcatga
     3001 gtaagttttt gtttaccatg accagggatg tctacatagc tatcacgagc taatgatgta
     3061 agtttgatat cattgtgttt gttatcaata gtaagtatca tcatagcatc tgaccttgaa
     3121 gaatcttcat ttggtctagc atctgttccc ataagtagta tgttaattat cccatcttca
     3181 tttttatggt cattgttatc caataaatca ctacttatgc tcgagtcatg aatagcagat
     3241 aatttataat aaaaatatcc atagacactt atagggaata ttactaccaa gaaagccagt
     3301 agtataacaa atttctttaa ttttgacaaa agtacccctt ctttcttctt tttcctgtat
     3361 attttttaat agcatacact taattatatt aaagtattg
//
LOCUS       DAEPLF010000291         3340 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEPLF010000291 DAEPLF010000000
VERSION     DAEPLF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.292"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            141..802
                     /locus_tag="KR314_003411"
                     /pseudo
     CDS             141..802
                     /locus_tag="KR314_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(1021..2058)
                     /locus_tag="KR314_003412"
     CDS             complement(1021..2058)
                     /locus_tag="KR314_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBH0329766.1"
                     /translation="MNKKLIKAKIFIWSILAISLSIVFISLLIGTIISHSFNFPFNTL
                     NPKYNYDNYIVAKNESFSDKINDINVDWISGEVRIKRSDSNKIKLIQKIPSNFSKKKL
                     AKIMVDGNSLSIYDNSTKKFFIGINLPKPTILELYLPNKLYDKITLYTTSADIYSTYI
                     RSNTVEANTISGAIGLSGNINKINLDTTSGHIDITKLNSKNATLKSTSGEIQLSGKID
                     VLNLETTNGNINLKDIYNKNLTCSSVSGKMNLSGVFSDIKAESTSSNIKINSSKMLSS
                     LYCETTSANVDLSIPDNPGFTLNFDKVSGVLDSDFELNRHSDLYTYKNGITNLYLSTT
                     NGNLTIIKNNH"
     gene            complement(2051..2737)
                     /locus_tag="KR314_003413"
     CDS             complement(2051..2737)
                     /locus_tag="KR314_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329767.1"
                     /translation="MLDKIRTYIDSSFTGVNETKKVKELKDELFENLKEKYNDQIQDG
                     KTEQEAYNSVISGIGDLSELIESVKEPYSLPSELIEEKKKRALRASIAVALFIISPFL
                     FVVSVVSFGIEPTIAILLMLILIALGVGLLVYNQMTRTNYALYNDNLSNKFKEFEDFE
                     ENYNHINPVYKSFMNAYWIIVVSIYLITSILFNIWSFSWILFIIGAAGVNIVKGIIQL
                     KGYKEEDKYE"
     gene            complement(2749..3069)
                     /locus_tag="KR314_003414"
     CDS             complement(2749..3069)
                     /locus_tag="KR314_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329768.1"
                     /translation="MIIASDMIRGHTETIILKHLLEKDSYGYEINKSILEKTKGLYEL
                     KEATLYTAFRRLEQSGAITSYWGDEVTGARRKYYSITNIGKEKYIKSREEWNNTKALI
                     DILI"
BASE COUNT         1208 a          415 c          478 g         1239 t
ORIGIN      
        1 taataacaat tcaaaataaa taacttatgt aagaaccgta ggaactacgg ggatagcctg
       61 tggagaatta gtaagacata tgttagtatg caaaattcta tgaagcagga accctgtgac
      121 tttagtcatg ggaggttcag attttggctg aagatgaaga aaataatcat ataaatattg
      181 agatgcaaag agcttttgag aacgattatt tggaaagagc tgaatattat cttagtcgtg
      241 tacatggtag aaagttagaa gaaggtaaag agtataaaga aattggaaaa actattggaa
      301 tacatatact aaatcatgta aaatataatc acatagaaga ttatgtaaat tgcctgagat
      361 taacaatgga cgagcatcca gatatttatt ctagtaaaac tgcattatat tttatagagc
      421 ttccaaaaat tcataaatct gactatatag taaatagaat tatgctatgg ggaaaactta
      481 ttgataatcc ttctagctca gatgtaagag ttatagctaa gaatgattct attattaaaa
      541 aagcacttga tagattaaaa gaattaggta gcaatgaaga atatttatta aatctgaaaa
      601 gaggtgctta tatcatgaat aaaagtagaa attttaaaga agagattgaa agagagacag
      661 caattagagt tgcaaaagaa aaagattata aaataattat aatgttgaaa aaaatggttt
      721 taaattagat gacatattga ataaatttaa tgattttgac ttaagtgaag atgagataaa
      781 agaagtatat gaaaataatt gatgataaat aaaaagtgaa cttatataag tttgctgaat
      841 ctgagcctgg aaatatttat gtgtattttt taatgaataa aaatttattt taaaaacaat
      901 aattagaggt acagttaatt atatttttat gatactgtac ttctagtttt ttctattgta
      961 gatttattat ttactaataa atctacaata gaagtgcttt tgttgtaaga atcattttat
     1021 ctaatgattg ttttttataa tagttaaatt tccatttgta gtgcttaaat ataaatttgt
     1081 aataccattt ttataagtgt ataaatcact atgacgattc aactcaaagt cactgtccaa
     1141 aacaccagaa accttatcaa aatttaaagt gaaacctgga ttatcaggaa tagacaaatc
     1201 gacattggca gaagtagttt cacagtacag agaagacaac attttgctag aattgatttt
     1261 tatattactg ctagttgact cagctttaat gtcagaaaaa actccagata agttcatttt
     1321 tccagaaact gaactacaag taagattttt attgtatata tcttttaaat ttatattgcc
     1381 attagttgtt tctaagttta aaacatcaat tttcccagaa agctgtattt caccagatgt
     1441 tgatttgagt gtagcatttt tactatttag ttttgttata tcaatgtgac cactagttgt
     1501 gtctaaattt attttattaa tattaccaga gagacctatt gcacctgaaa ttgtgttagc
     1561 ttcaactgta ttacttctta tgtaagtgct atatatatct gctgaagtag tatataaggt
     1621 tattttatca taaagcttat tgggcaaata tagttcaagt attgttggtt ttggaagatt
     1681 aattccaatg aagaattttt tagttgaatt atcatatata gaaagagaat ttccgtcaac
     1741 cattattttt gctagttttt tctttgagaa attactaggg attttttgaa ttaatttgat
     1801 tttattagaa tcacttctct taattctaac ttcacctgaa atccaatcaa cattaatgtc
     1861 atttatttta tctgaaaaac tttcattttt agccactata tagttatcat aattgtattt
     1921 aggattaaga gtattaaaag ggaaattaaa agaatgtgaa attatagttc ctataaggag
     1981 tgagataaat acaatagaaa gtgatattgc caggatactc cagatgaaaa tctttgcctt
     2041 tataagcttt ttattcatat ttatcctcct ccttgtagcc ttttagttgt ataattcctt
     2101 tgacaatgtt gacaccagca gcacctatta taaatagtat ccacgaaaag gaccaaatat
     2161 taaatagtat gcttgtgatt agatatatag aaactactat aatccaatat gcattcataa
     2221 atgatttata aactggattt atatgattat aattttcctc aaaatcctca aattctttga
     2281 atttatttga taaattatca ttgtatagag catagtttgt ccttgtcatt tgattataga
     2341 ctaataaacc tactccaagt gctattaata tgagcattaa aagaatagct atagttggtt
     2401 ctatgccaaa acttacaact gagactacaa ataggaaagg gcttattata aaaagagcaa
     2461 ctgctattga tgctcttaag gctcttttct ttttttcttc aattaattca gaaggtagag
     2521 aatatggttc cttaacactt tcaattaact cagataaatc accaattcct gagattactg
     2581 agttatatgc ttcttgttct gttttaccat cttgtatttg gtcgttatac ttttctttta
     2641 gattttcaaa taattcatct ttaagttctt tgactttttt tgtctcatta actcctgtaa
     2701 agctactgtc aatataagta cgtattttgt ctaacatatt ttcacctcct aaattaatat
     2761 atctatcaat gcttttgtat tattccattc ttcacggctt tttatatatt tctctttacc
     2821 tatgtttgtt attgaataat actttcttct agctccagta acttcatcac cccaataaga
     2881 ggttattgca ccagattgtt caagtctacg aaatgctgta tacaaagtag cttctttcaa
     2941 ctcatacaat cctttggttt tttcaagtat agatttatta atctcataac cataactgtc
     3001 tttttcaagt aaatgtttta agataattgt ttctgtatga cctcttatca tgtcagatgc
     3061 gattatcatg aaaatcacct ccaattaaat atataatact ttgatatact tcgcctgtca
     3121 aggtatatca tgagaaaaga cattttattg taatatttat aaggttttat atagaaataa
     3181 tgtaaaaact ttataaataa tatattttat acctagttat agataacttt atatagttat
     3241 tattaaaaaa agcaataaaa agtttaattc aatttagcaa atttagtgat ataattaaaa
     3301 aagaaaagaa atatggactt atttggaata attttgacaa
//
LOCUS       DAEPLF010000292         3299 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEPLF010000292 DAEPLF010000000
VERSION     DAEPLF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.293"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(190..567)
                     /locus_tag="KR314_003415"
     CDS             complement(190..567)
                     /locus_tag="KR314_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0329769.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
     gene            complement(754..1725)
                     /locus_tag="KR314_003416"
     CDS             complement(754..1725)
                     /locus_tag="KR314_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0329770.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            complement(1759..1956)
                     /locus_tag="KR314_003417"
     CDS             complement(1759..1956)
                     /locus_tag="KR314_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBH0329771.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            complement(2024..3235)
                     /locus_tag="KR314_003418"
     CDS             complement(2024..3235)
                     /locus_tag="KR314_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBH0329772.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKYDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIVFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
BASE COUNT         1158 a          534 c          352 g         1255 t
ORIGIN      
        1 aatcatttct tatttaaata agtggtatca tttaacttga tactccctat tccaattatt
       61 ttaggtattt tactaattag agaacctatt taatattact ttatgaatag tgagtagctg
      121 attaaataat accatttatg gttttcagcc atagattaat taaattctta gagttttatc
      181 ctgatataat tactcttctt ctaaattttt aaaatgactt tcaaataaat ccaacatatc
      241 tttatcaaca acttcatcat catgtagtgc tgaaattaaa ctctgtaaag aattgtcgtg
      301 tatattgctt aaaaaatctt ttgtctcaac acttaaatac tccttatgtc caacaactat
      361 tgtataatga gtgtatctat ctattttttc tgcacttaaa aaatgttttt taacaagtct
      421 tgataaaagt gtcaatgtag ttgtttgttt ccaaccatat tttcgttcca tagcttcaat
      481 agtttctttt gatgttactg tatcattttt ttcccaaata aacttcataa cttttaattc
      541 tgcttgtggt atttttgaaa ttgtcataac cttacgcccc tctcaaactt ttattaaatt
      601 aatactatcg caatatagtt tttaagtcaa agattttgtc gataataata ttttccaatt
      661 tttaccatag agttattttt tatataattc tatgataaat tacataaaaa atatttattt
      721 tacatcataa aattagaaat gtaaatagta tgattattta gtcttatctt taataaacaa
      781 tccagtaaat ttatacatcc aaagtgcatt cataaacaac aataaacttg ttgaaaaaaa
      841 tactgttcta atacctaaat gtgctgatat ttgtccccct aaaaatgctc ctgatgaaca
      901 acctatatac tgggcagatg cattatatcc aaatattttt cccacatatt cttctggtgc
      961 aattttttta agcaagctgt taacagaagg attaagccct gcaactgaca accctaataa
     1021 aaatctcaac aacatcagtt gccatgcagt ttttacaaaa gcttgtggta taaatataat
     1081 cccagcccat aaaagtgaaa ttaacaatac tttttgagga cctattttat ctgacatttt
     1141 tccaagtttt tgagctgcta ataaatttgc taatcctgat gcagaaaatg tcaatccagc
     1201 tattaaagct acatttgatg caaaatttgt aagttgtgat atatatatgg ttacaattgg
     1261 ttctatagag tataaagcta gttgagtaat gaatgtggtt acaaaaatag atgcaattaa
     1321 aaatttattt ggcaatatat ttaattgttc tttaggtata gaaattgatt cttttatttc
     1381 tttaggttta aaattatcta ctacaaataa aatagagatg ataaatgata caaacaacaa
     1441 tcctcctgtt ataataaatg tacttttaag acccaagatt gattctatat atcctcctac
     1501 tgttggacca attaatgagc cagtagttga tgcagttgca agagtaccca aagcccatcc
     1561 tgaatgattt ttatctgtct gtgttgcaat aagagtagta catgcagtag cataacccgt
     1621 aaaaactcct tgaagtattc ttaatcctaa taactgataa acattttgaa caaacgccat
     1681 ggtaaatata actattccca tcccaatact tgcacgtaaa agcattgatt ttcttccata
     1741 tttatcagca gctttccctc aaattggcga aaagaatgct gctactacaa aggtacatcc
     1801 aaaaattata cctgaatact gttgtattaa actaacatca gttaccccca actgtttaat
     1861 atataatgga agtataggtg ctatttggct catccctata gaactaaaaa acatacctat
     1921 ccaacataca aataaatttc ttttccacaa ttccatatag aattacaccc cctgcatttt
     1981 aaattttttc aataatacta agtttattaa tttatcctat tgtttactca atatatccat
     2041 cttttctttt ataatattgt ttaggaaatt cataactcga aaataatagt ggtgataaag
     2101 aataggagta acttccagca ttactaattg aaatcaaatc tcctatattt gcttttttca
     2161 atttaacatt atttgcaatt acatctaatg atgtacagag attcccaaca acatttacat
     2221 actcttcttc ttttaaatta tttaatactg aaagagaaaa ggcatgattt gatgtatata
     2281 atggctcttg accttctaat tctactccac aagtaatatt ttcaattaaa ttagaaataa
     2341 taggtctcat aaatccatta agtccatttt ttacaataag atattttttc cctcttgata
     2401 ctttaatatc tacaatttta acataataat tacctgattt acaaactatg aaacgtcctg
     2461 attcaataag aagtcttgat ttcagtgtgt ttgaattagt aaaaacaatc ttttgagtta
     2521 attttgaaag ctctttaaga ttaagtggat tttctctaag attatcataa gctactccaa
     2581 gtccactacc aaaatttata aattcaatat taatatactt acttttattt aaatttaaag
     2641 ctataaaaaa acatttttca taataatgtc ctaacttttc aaaatccaat acctgagatt
     2701 taatatgtat atgaatccca actacagata cataatcaca catacttaac atcttaaaaa
     2761 cactgctcat atcttctata tctataccaa atttgcttga tttagcacta tcatcatcca
     2821 tagtaaaatc aggatttatt cttattccta ttttaacttt ctgattatgc tttttagata
     2881 tcttatttat aagttctatt tctgtaatac tatctgcaat aattatgcat ttatctaaaa
     2941 cagtttctat atcaatctct gttttgcctg gtgatgaata ataaatatca tatttagaaa
     3001 ctccacattc tgatgaaatc ataacttcat tagttgaaga agcatctgct ccaaaacctt
     3061 gctctgcaat gctttttaca attttgttat atggatttgt ttttatagaa tataaaaaat
     3121 caaaacctaa aatattattt ttcaattcct tacatttttc aattatatta gtctcatcat
     3181 atatataaca tggtgcattt tcttctataa atttatagtc ttcatttgag tacatatttt
     3241 ttacctccaa gattttaaat ataatcataa attaagcaat tataatccta taagataat
//
LOCUS       DAEPLF010000293         3292 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEPLF010000293 DAEPLF010000000
VERSION     DAEPLF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.294"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(420..769)
                     /gene="ssrA"
                     /locus_tag="KR314_003419"
     tmRNA           complement(420..769)
                     /gene="ssrA"
                     /locus_tag="KR314_003419"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(927..1382)
                     /gene="smpB"
                     /locus_tag="KR314_003420"
     CDS             complement(927..1382)
                     /gene="smpB"
                     /locus_tag="KR314_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBH0329773.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            1981..2808
                     /locus_tag="KR314_003421"
     CDS             1981..2808
                     /locus_tag="KR314_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBH0329774.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
BASE COUNT         1143 a          489 c          422 g         1238 t
ORIGIN      
        1 tagcatccca catagataat aaatttgttc tatacttata cataatctta taggattctg
       61 tatatcatta taactagtaa ttactctaat agattaagta gcacttatat gctttaaact
      121 tctttatcct tttaatcata agtttaaaca agttatatat ttcctagatt gaaataaaaa
      181 gcataatata aaaacaattc atcagtataa aatatttatt gtattatcat caaattcact
      241 atatctctaa aagtattctt tatatgttcc atggattaat caaatgcacc ccaatatacc
      301 ccaaaaaaat ctctttttct gtggttgggg cacggttggt ggatttcatc tccaaaacaa
      361 aaaacactag tcaataaaga ctagtatttc caagtatttc atcattcttt tttaaaaatt
      421 ggtggagacg aggggagtcg aacccctgtc cgcaaatctt ttccttgctg catctacatg
      481 cttagccaac ttcactaata tctcacctca catagcgcca agtggcaggc tctatgctta
      541 gccagtccca attaatctac ttcaggtcga gacaccccta aagtagttcc ctgcataatg
      601 acgtccagtc ttagtcggca ggaggactaa gagggacgag cagcaactag gctgctatcg
      661 cgaaattatc gtctgcgttt attttaaagt tccccagttt ttacgtgacc cagagtaaca
      721 cgacatgcag cctcaagttc caaacccacg tcgaaacctt tacgccccct tgagtagttt
      781 atagtccagt agacttttcg caaactttat caccattttt attaaatttt taatgtatat
      841 ataatataat acttttaaga ataaaaatca atgttatgtt ctagataata aagaatttta
      901 ttctaaaaaa tacagtttta tctagtttaa tacttacctg tcatttctct ttctatacgt
      961 ctttgtgcat ctttcttagc catatcttga cgtttatcat atagtttttt accttttgct
     1021 aaggcaatct ctactttaac tttaccgttt tttagataca aactaagtgg tataagtgaa
     1081 taccccttta tagtagttgc tccaataagt tttcttattt catgcttatg aagtaatagt
     1141 tttctaactc ttaaagaatc aacattgaat atatttcctt gctcatatgg acttatatga
     1201 acttgtttta aaaatacttc actattatct acagatgcaa atccatcact taggttaact
     1261 ttgccttgtc ttattgattt tacttctgtt ccttttaatt caattccaca ttcataagtt
     1321 tcttctataa aatattcatg tcttgctctt ttgtttgttg ctaatatttt tttaccagcc
     1381 atataaatct caccttcttt gttagaaatt tattccataa atgttagaaa tttactaaat
     1441 atttaatgtt gccaaattat ttgactaact tgtatgataa catatttagg cattttagtc
     1501 aatatttcac tgatatatta agttagttag gctatatata attaagtaat ataaagatat
     1561 gaaaatagct aaaaaactac ttcgaatata cttaatacta aactaacttt aaccagtaac
     1621 actgatttct aaatttcaaa acaaaaatat tacatattat aaattttcta tcttctatct
     1681 tctatcttct atcttctatc ttctatcttc tatcttctat cttctatctt ctatcttcta
     1741 aataatttta acatgcccat aaaatataat acccacaaat gataaataat actcaaaata
     1801 tctattgaaa taactataca tccttaaata acttaactta aatctaaaca tttcaaaaac
     1861 ttcttaaaaa tatgtaaaaa tttgatgata aagtagcgat tttttcaaaa aaaatgtaat
     1921 atagagttat atcttataat attttagaga taaattttat catagttggg agaaatttgt
     1981 atggatttaa gcaaaaaagt aaaaatttta gaaaatgcat cagatggcaa aagcgaattt
     2041 gaaatattag aatttgaaaa acttgaggga gcttctagcc cattaatggc aatgggatta
     2101 tattttgcta gagaagctgg tttgaaagct agacaaatta agataaaatt aaataattca
     2161 tcaataaaaa cagaagctgg tgcattatac tattataaag gaaatatatc ttctgaaact
     2221 aagattggtg gtgttggagg tctttttaaa aaaggaatat caggagcact tactaatgaa
     2281 tcaatgatga aacctgtata caaaggtact ggagaagttt atttggaacc ttcttttaga
     2341 cattatttat ttatgagtct tgataatgat tctataattg ttgataaagg attattctat
     2401 tgttgctctg atacaataga agtaaaagca tttacacaaa agaacttctc atctgcttta
     2461 cttggtggtg aaggcttctt ccaaatagaa ttaagtggta ctggtgtaat cgttctggaa
     2521 tgtgttgtac ctcaaagtga aattgtagag tatgaattga aaaatggaga agaattaaag
     2581 gtagatggta actttgcaat agcaagaaca tctggggtta atttttctgt tacaaaatca
     2641 gataaatctt taattggttc tgctttaaat ggtgaaggtt tcttaaatac atttactggt
     2701 aatggtacag tttggctagc tccaactcaa gtaatgtatg aaaaactaag ttttggagca
     2761 atgcctacta atgataatat gaataataaa gtttctaagc aaaaatagtt gttttataaa
     2821 tatttattaa atcattattg attttataaa gtcactataa attttattag attattatta
     2881 taaaatcaga gtgtggaaat gtttatgtgt attctttaaa taaaaatttc cacattctgt
     2941 aaggttatta tacttgatat ataaaattca atttaggaac atagtctaac atttataaac
     3001 atattaaggt aatgatttct cttggtaatt attctcatat cttattcttt gattatattt
     3061 atcttattcc tagcccttat gtcatattat atttcttatc ttattattta taattagcat
     3121 taacttatct aaaatcttgt atttttttaa tagtaaagtt tacatctata ttaaaaagtt
     3181 tacatctata ttaatttcat aaaacaatca aggtagtcaa gagattcaac tttatttgat
     3241 tttaaaaatt taagtgaatt tgttcttata cacatctttt taaaattata tc
//
LOCUS       DAEPLF010000294         3244 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEPLF010000294 DAEPLF010000000
VERSION     DAEPLF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.295"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            430..1872
                     /locus_tag="KR314_003422"
     CDS             430..1872
                     /locus_tag="KR314_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0329775.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1914..2267
                     /locus_tag="KR314_003423"
     CDS             1914..2267
                     /locus_tag="KR314_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329776.1"
                     /translation="MKKSNIFSIIFPTIIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2458..>3244
                     /locus_tag="KR314_003424"
     CDS             2458..>3244
                     /locus_tag="KR314_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBH0329777.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFV"
BASE COUNT         1256 a          332 c          557 g         1099 t
ORIGIN      
        1 cttcaagtgt taccttaggt atttaagttg atagtggttt gcacgtaata aacttgatgt
       61 aatatatatt cttaataata tcgataaaag atttacttaa aaataatata gattatgtaa
      121 acatataatc tatttattgt atacaataaa atttcatttt gtaaaacagt gaattaaata
      181 tgtaatatag atatcgattt ttattacaaa ttgttactaa atataggtac attagaacaa
      241 aaatctagta aattgtaact aaaatgtaaa tttttccttt aatctataca atattatgga
      301 aactttttgt aatttatgat ataataacaa atatataatt attaaattat aataaatgac
      361 tatagcgtag atgtaatact atgttatagt catttttgaa ttaaacttga tatatcaagg
      421 gggaagaata tgagaacaat tagaagagtt ttagcattag gtttaacatt agctatattt
      481 ctaataaatg tacctaatgt agatgcatta acatcagata caattaaagg taacaatata
      541 tatgagacgg ctggattaat agcagataaa aagagttatg atacagcaat tatggtaaat
      601 atggataatt ctatagcaga tggtctttca gcaagtggtc ttgcaggtgc tgttgatgct
      661 ccaattttgc ttgcacaaaa aaataagata ccaaatgaaa caaaacaaag attaaaaaat
      721 gttaaaaaga tatacataat aggtaaagaa ttatcaataa gtaagtcagt agaaactgaa
      781 ctaaaaaaca caggggcaca ggtaactaga ttgggtggag atgacagaat aaagactagt
      841 tatagtgttg ctaaagaggt aaatggtatc aaaaaagttg atgaagtgat attaactaat
      901 gcatataaag gagaagcaga tacaataagt gctgcacccg tatcagtaag agatatagct
      961 cctatagtac ttacagatgg aaagagtgtg cctttttcaa caagcaatgt aaaaacatat
     1021 gcagtaggtg ggagtatatc aatgagcact agtttagtta ataaaactaa tgctaaaaga
     1081 cttggtggtt cagatagata tgatacaaat aagaaagtta taaaagagtt ttatccagat
     1141 gcatcagaat tttatttgag tgatggatat gatttagtaa atgcacttac aggttctaca
     1201 attgctaagg aaaatccaat tgtattggtg tcagaaagta gtgataagtc tatattagca
     1261 ggagcagata agattactag attaggttca ataagtgaca gtgtgtataa taagtgtgtt
     1321 tctgctgcac aaaataatgg cgattcgtct acaaaaggtg agtcacctat gaaaaatgag
     1381 acaagcatat taggtcagcc aacagctagt ttagaagcgt gcttaaaatg ggcaaaatct
     1441 aaaaaagcta atgatttatt tatagagtta ataccaatat tatatgatac tgctgttcag
     1501 gaaggtgtta atcctgtttt ggcagtagct caatctgcaa aagaaactgg tttttgtaat
     1561 tttggtggag tattagatgc atcatttaag aatccttgtg gacttaaaac ttctgtaggt
     1621 ggttctgata ctgataagaa tgcccattca agatttgata cttgggaaga aggaatatta
     1681 gctcaaattc aacatttatg cttatatgca ggacaagatg gttacccact ttcaaatcca
     1741 gtagacccta gacatgaaaa atctttattt ggtaaggcta aaacagttga aagtttatct
     1801 aataattggg ctggaggtca atatggacaa gatttagtaa gaatgatggg agaaattgag
     1861 gcaacaaaat aattgaagat tatcaaatat ccaataaata aggattgatt attatgaaaa
     1921 agagtaatat ttttagtatc atatttccta caatcataat ggtgctaatg tttttaactt
     1981 ttgctacaga tatatttaat attccagata ttcaatctaa aagtattttt gttgtgggaa
     2041 tggttttgat ttttccaatt tcttttttaa tacaagggat aatttgtgta ttaaataaga
     2101 ctaattggat tttatctctt attgtgtctt tagtaactta tatagtatta atgtatatat
     2161 ttttaaatga ttctgcatat atatatgttt tactttatac agctttttat atgattggac
     2221 atataacagc taaactctat tacaaaatta agacttttaa aaattaaatt gaagtatata
     2281 aaagtaacat taagagaagt atccttaatg attttctaaa atcagaaata ggtgcttctc
     2341 ttttttgtaa aaataaaagt atgtaaataa aatttaacca ttataatttt cttttaacaa
     2401 atgtatacaa taaacttgtt gtataattaa gatagataaa ataaagggag ggataatatg
     2461 aatttaatag ataatcatat tcatactaat ttttcaagtg atggaaagga ttccatggaa
     2521 gatacaataa aaaaagctat aagtattggt gttagatatt tgacttttac agaccattta
     2581 gaacatgatg aagatagagg attttgcata aattataatg actatgttcc agtatttaat
     2641 aagtttaaag aaaaatataa aaaggatata gagttattac taggtgttga agtaggttac
     2701 agaaaacatc taaaaaatga aatagaagaa ataataaatt ctaatccttt tgattttgta
     2761 ttgtgctcaa ctcatacaat tgacaatgta cctgttccat caaaggcgta ttttaaaggt
     2821 cttagcaaag aagatgcgta ttataaatat tttaatagca tacttgaaac taatcgtgaa
     2881 tttggagatt acaatatata tggtcatttg gattatatat ctaggtatgg aatatattca
     2941 gataatagag ttatatacaa tgactttaaa gatgttattg atgaggtctt aaagtcaata
     3001 atcaataatg ggagtggtat agagctaaat acttctggtt atagatatgg attaaatgct
     3061 atacatccaa atgaagatat tctaaaaaga tataaagaac ttggaggatt tatagttact
     3121 gtaggttctg attctcatag ggtagaagat atatgtaaag attttgatgt ggcctatgat
     3181 atgcttaagt atcttgactt taaatatgta tctttattta aagaaagaga aacttatttt
     3241 gtaa
//
LOCUS       DAEPLF010000295         3244 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEPLF010000295 DAEPLF010000000
VERSION     DAEPLF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.296"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            65..259
                     /locus_tag="KR314_003425"
     CDS             65..259
                     /locus_tag="KR314_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBH0329778.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIREFQDTIIL"
     gene            complement(361..870)
                     /gene="vanZ1"
                     /locus_tag="KR314_003426"
     CDS             complement(361..870)
                     /gene="vanZ1"
                     /locus_tag="KR314_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBH0329779.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1190..1580)
                     /locus_tag="KR314_003427"
                     /pseudo
     CDS             complement(1190..1580)
                     /locus_tag="KR314_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            2024..2593
                     /locus_tag="KR314_003428"
     CDS             2024..2593
                     /locus_tag="KR314_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329780.1"
                     /translation="MKLRNPKIKKLLSVALLAALIGIPIGQTANAMEISNNVPSYIEN
                     NPTTIEDLEINDSQLPKPGTVTYIDINKLIKNLKENGIDTTLIENELYYRKAGTNKIV
                     WTKTGFDLYLSKGVALTLAGAGTGVAGLLAGAIPGIGQYLAAAIVGAITMHLGSNITS
                     GKIFKFKKMRYNKGGAYVTKYYLQSVRNQ"
     gene            2885..3202
                     /locus_tag="KR314_003429"
     CDS             2885..3202
                     /locus_tag="KR314_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329781.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYISIGLFTRRLDITIIAIILVATITVILYYLVRKRY
                     ITI"
BASE COUNT         1219 a          412 c          382 g         1231 t
ORIGIN      
        1 ttattttcag aaaagtgata ctattgaata tattaagaat aattagagat ggaggaacta
       61 agatatgagc gtaattgtaa tgtgtagtcg ttgtggaaaa gaaatagata aatatagaaa
      121 taaatatata acttattatg agaaaaatac caatgcagaa attaaagttt gtataaattg
      181 tgcattagat ttattagaaa aaacaagaga aaatcaagaa ttgaacatac gagagtttca
      241 agatacaata attctttaat aatttaggaa agcacctata taggtgcttt ttatgtgtaa
      301 ataattttat atgtaactgt atcttacgtt ctattttaag ctaaacctat atacttattt
      361 ctataagttt gcaaaaataa ctaatcctaa aaataataca acaataattg tcacaatcaa
      421 tgccaataca ttgataattt ttatggtatt attcttaaat attttcgata atagcataaa
      481 aacagctatt cctatgattc ctcctagtgt gtttcctatc aaatcggtta tatcacttgc
      541 acctagtgca aatatatact gaagtgtttc aaatgctaaa ctagttataa aaataggtat
      601 aacttttttt ataaagctcc attcttcttt tagcatacac acatatatcc caaatggtac
      661 aaatatagcc acatttaata taatctctga tatatctact ctattattaa ttataagtga
      721 cccagcaaaa ggtactaagt ttatgcttct aagattcatg gtttcaagag atgagatatc
      781 aaattgcaac ttaaatagta ttatccatgt taaaataatt atgtaaacta taaataatcc
      841 ttttgtaata ttatgttttc tagatttcat ttattacctc cttactatat tattgtatta
      901 ttttattaat acaataatat agtaaggtaa tttatctgtc aactatttct tctaaaatta
      961 tattcctttt ataatttcaa tatctttatc atatttacat gatttaagct attgtagtat
     1021 ttcatatatc tttaaatttt aattggtatt attctctata ttttttacta tcaaacacta
     1081 aggaaataat cacttttctt tatataatgt tttaccatca cataaaacta acattaaatc
     1141 caaacgctac tctcaattgt ctcaaactaa aaagatactg atagctttat tagtcatcaa
     1201 tatcttcttc tttcaaattt ttaaagtatt cttctaattt gtccaagctc ttactatcta
     1261 aaacttcatc atcatgtagt gctgaaataa aactcttaaa tgaattgtta tgcataaaac
     1321 taaaaaaact ctttgtctca acttttaagt aatctttctc ttttatagct atagtataat
     1381 gcgtacatct atctacctta ataagtagtt aaaaatccct tatctactaa agtttttaaa
     1441 aatgtcaatg ttgtgctctt tttccaatcg tatttcaact tcattgcttc tatgatatct
     1501 cttgaggcta atacatcatc tgctccccaa atatacttca ttagttttag ttctgcatgt
     1561 ggtaatttat taatcttcat tagtttacac ctcttaccaa tttatgtata attaaatttt
     1621 atactaataa tagtatgtaa tcaatcagat tggaacaaat ataatattcc aatttattcc
     1681 atataaaaat aattaataaa aaagaagtac tctactttag agagtacttt tggattatat
     1741 attcaaaaat ttatctaaca tgattagagt atgttttatg ctttagtgtg ataattttag
     1801 ttcgttttat tattataata tgtattatat ttcattataa tctataagct taaatacagg
     1861 atatttattt catatactta atttatatta agttttatta tatgtattct taagttttac
     1921 tatatttttt attaatattt aatgtagtta attcttaaga attatattct acaataataa
     1981 tatatatctt tgatatataa ctaatttaaa atggaggtat tttatgaaac taagaaatcc
     2041 aaagattaag aaattgttat cagtagcatt attagcagct cttataggta tacctattgg
     2101 acaaacagcc aatgcaatgg aaatttcaaa taacgtccca agttatatag aaaacaatcc
     2161 aacaacaata gaagacttgg aaatcaatga ctctcaatta cctaaaccag gtacagttac
     2221 atacatagat attaataagc taattaaaaa ccttaaggaa aatggtatcg acactactct
     2281 tattgaaaat gaattatatt atagaaaagc tggaacaaat aaaatagttt ggactaagac
     2341 aggctttgat ttatatcttt ctaaaggagt tgcattaact ttagctggtg ctggtacagg
     2401 ggttgcaggg cttttggctg gtgccattcc tggtatagga cagtatcttg cagctgctat
     2461 agttggagca attacaatgc atcttggttc aaatataaca tctggaaaaa tctttaaatt
     2521 taaaaaaatg cgttataata aaggtggggc ttatgtaacc aaatattact tacaatctgt
     2581 aagaaatcaa taggtgataa aatgattata aaatatctac tttttgcaat tatctcagca
     2641 ctagttttat tcttttgtaa taaagtattt caactaagta aattacacaa gttcattata
     2701 ttgtttgttt cattttttat actaactttt gtagcatcat ttattaatag ataaaaaata
     2761 agatggtggg gttaacctac catctttttc tcatttaata tttgtaatat tttagatact
     2821 ctttatactt gtgttactga tatactttga ttaagaaaag taaatttaac aggaggtatc
     2881 tgctatggat tttggaaata tcaatcttat acttattgga ataatagtta taataggaac
     2941 tacaataata tatttaatta aacctaaaac tgctttttgt agtaaaaaat attttaataa
     3001 attagaatca atatatggaa atatagataa gaaaaaaact gttaaactag aagtacttta
     3061 tcgctatgta acaggtcttg agtatatatc aataggctta tttacaagaa gattagatat
     3121 tacaataata gcaataatat tagtagctac tataacagta atattatatt acttagttag
     3181 aaagagatat ataactatat aaaatctaat agaattaata tcaaaagaaa gtagcaacta
     3241 atca
//
LOCUS       DAEPLF010000296         3221 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.297, whole genome shotgun sequence.
ACCESSION   DAEPLF010000296 DAEPLF010000000
VERSION     DAEPLF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.297"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            11..1231
                     /locus_tag="KR314_003430"
     CDS             11..1231
                     /locus_tag="KR314_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0329782.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISSTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            1268..2617
                     /locus_tag="KR314_003431"
     CDS             1268..2617
                     /locus_tag="KR314_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0329783.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYSLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWFIRIISWSTL
                     AVGFATALGSFWPESATEYKGCIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFVIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     IALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(2749..3042)
                     /locus_tag="KR314_003432"
     CDS             complement(2749..3042)
                     /locus_tag="KR314_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329784.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
BASE COUNT         1227 a          427 c          480 g         1087 t
ORIGIN      
        1 aggaataaaa atgcaacagg ctttgaaatt aaaatatcaa accaaagaat taggaaaaga
       61 ttttgtaata gaaagttgta attctataaa accatggctt ataaacataa gaagagaatt
      121 acataaaatt ccagaattag ctcttgaaga aaatcttact aaacagaagg taatttctta
      181 tttaaaagaa atcggaattg attatatgga atttacaaaa cacaatggaa ttatggcata
      241 tattttaaaa gaaagtgcag ataaaacgat ttgtataaga gcagatatgg atgctcttcc
      301 tattgaagaa gaaaacaaca taccttataa atcaatacat tctggaaaaa tgcatgcttg
      361 tggtcacgat gcacatacaa ccatgctact tggagcatgt aaagttttac attcaataaa
      421 agataaactt aatgtaaatg taaaattctt atttcaacca gcagaagaag gttttggagg
      481 agcaaaattt ttagttgagg atgggtgttt agaaaacccc aaggccgatt atatattcgg
      541 acttcatgtt atgccacata tagaaacagg ccttatcgaa acaaaatatg atacattaaa
      601 tgctagtgta gacactataa aaataagtat caaaggtaaa agagctcatg gtgcatatcc
      661 tgaaaatggt attgatgcaa tagttacagc ttcacaaatt gttacatcat tacaaactat
      721 aataagtaga aacttagaac caaataatgc tgccgttttg actataggta aaatatatgg
      781 tggtgatgca cacaatgtaa tatgtgaaga tgtaaaacta gaaggcactt taagaacctt
      841 aaatagcaaa acaagaaact ttatgattga taaaatagct aaaattgttg gagacactgc
      901 atctgcattt ggttgtgttg ggactttaca tgtaagtgat gaaaactatc ctgctgttat
      961 aaatgaaaaa gaattagtag atacagttat atcaagtact aaagaattac ttggagagga
     1021 aaaatttata ttgagaccaa acccgtcttt aggtggtgaa gacttttctt tttacacaga
     1081 acattgtaaa ggagcattct tccatttagg atgtaaaaat gaggaaaagg gcttaatttc
     1141 acctcttcat acctctagct tcaatataga tgaagattgt ttaccaatag gtgttatgat
     1201 gcatgtaatg aatactttgt attttaatta gtacagtaat taataaaaga ataggagata
     1261 aattaatatg gaaaaagcta agaaattagg tctatttagt atgatattat tagggattaa
     1321 tagcataata ggttcaggta tattcttact accaggtaaa gtttatagtc tagcaggtca
     1381 aaacagtatg tttatataca tttttgctac actcttagtt ctatcaatac ttttatgttt
     1441 tgcagaagtt ggaagcatgt ttgataagaa tggaggagca tatctatact ccaaaaaagc
     1501 ttttggagat tttataggat ttgaagttgg tacaatgtct tggtttatta gaattatatc
     1561 atggtctact ctagcagtag gatttgcaac agctttaggt tcattttggc cagaatctgc
     1621 cactgaatac aaaggttgta tagctgcaat actcgtaaca ctactttcaa taaatagctt
     1681 atttggaatt aaaagcacaa agataatgaa taatgtaata actatagcta aattagtacc
     1741 attaatagtt tttgtgatag ttggaatatt ctttataaaa tttgtaaata ttgttccttc
     1801 aggaaatgta gttaactcta gtatgggtcc agccattata ttagtatttt acgcatttac
     1861 cggctttgaa tcatttattg tggcaagtgg agaaatggaa aatcctaaaa aaaatcttcc
     1921 aatcgcatta ataaccacaa tatttatatg tgcaataatt tatatattaa ttcaaattgt
     1981 ctgtatgggt atacttggag atagactatt tgaaaacagt attccaattg cagatgcttc
     2041 aagtgtattt ttaggcaatt atggtaaagt tttcatatct gtagcaacat taatatctat
     2101 atttggtata aatataggtt catcaatagt aactcctaaa tgtggttctt ctctagcaga
     2161 agaaggttct cttcctgcat ttataggaaa aacaaataaa tatggtgctc catatgtagc
     2221 aataataata tctttaattt gttgtatacc acttgtgcta actggaagtt ttgaacaact
     2281 tgcagtaatg agtgtaattg caagatttgc tcagtacata ccaacttgtt tatcagtaat
     2341 agttttaaga aaaagaacag atgttaaggc ctcttttaag attccatttg gtcctgttat
     2401 accaatggta gctattttag gtagcttatg gttgttacaa caagcttggg cagaagatat
     2461 ttcaaaacct ataactcaaa atagagtctt aataggatta ggggcaatgt tgttaatagc
     2521 tcctctatat atatttatga aaaaaaataa ggaaatcgag cataaaaata aatcagaaga
     2581 ttctaaatta tatatttcga gagaaaaaat tcaataatat tttatactat tacttctcat
     2641 aaaatataat aaatataaaa aaattgtaga taaaactatt ttataaaatt tatctacaat
     2701 ttttttatat aaacatatat caaaatcaaa atattattaa tttatacttt aaagattccc
     2761 atctttatca tattttattc ttataagaaa atataatcct attactacta actctatcac
     2821 taaaaatact ccataggtaa gaattgaatc aatactatct aacccatttc taaaaaaaat
     2881 taatgctata cctgctgcta taatccctat agcatttttt cttactgcat tttgcccaac
     2941 tccataaaaa cctggcattt ttccttttat ttcgacagca gttaaaatta tcccaaacac
     3001 tataaataat actccaataa gcatcataat catactaatc atctaattac ctccttcact
     3061 taaggaaatt tcaatttcta aaatctaaaa taaataatat ttatttttta taataatatt
     3121 gtatatccaa ttaactattt tggaaattag taccaataaa taatgtttaa atattgaaat
     3181 aatatattaa ttctaatcaa tattattaat atatatacat t
//
LOCUS       DAEPLF010000297         3189 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEPLF010000297 DAEPLF010000000
VERSION     DAEPLF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.298"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..1425
                     /locus_tag="KR314_003433"
     CDS             <1..1425
                     /locus_tag="KR314_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBH0329785.1"
                     /translation="KRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGKIADK
                     NAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKVYIIG
                     GTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAYKGEA
                     DAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTRLGGS
                     TRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPIVLVNDGSNKSILKNA
                     KKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDISAGEY
                     LITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMTLAKY
                     AKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTNDTFS
                     GRRYITVEEGQYLILNDCYLIENE"
     gene            1673..3109
                     /gene="miaB"
                     /locus_tag="KR314_003434"
     CDS             1673..3109
                     /gene="miaB"
                     /locus_tag="KR314_003434"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBH0329786.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT         1337 a          326 c          559 g          967 t
ORIGIN      
        1 aaaagaatat taacattgac tttaagtatt tcttttgtat ttgcaaatat tcaattagta
       61 gatgcaatat catctgtaga aaaaatacaa ggaaaaaata agtatgaaat agcaggcaaa
      121 atagcagata aaaatgcata taaaactgct atattaatta atactagtaa ctctattgca
      181 gatggattaa gtgcaagtgg actagcagga gctttaaatg cccctatact attaactgaa
      241 aaaaatacta taccaactga gacatctgca agattaaaaa atgtatcaaa agtttatata
      301 attggaggaa catattctat tagtacatct gtcgaaaatt cattaaaatc taagaaaatg
      361 aaagttgtaa gaattaaggg aaatgacaga ataaaaacaa gttataatgt tgcaaaagaa
      421 ataaattcaa taaaaaaagt caatactgtt atgctaacca atgcatataa aggagaagca
      481 gatgctataa gtattgcatc tgtagcagca agagataaag caccaataat tcttactaat
      541 ggacaaagta tacctttttc aactagtggt ttaaagtctt atgtaatagg aggaacagct
      601 tcaatgagta ccactttagt aaacaatact aaatctacta gactaggagg gagtactcgt
      661 tttgaaacta ataaagcaat aataaataag ttttataagg atgctagaga gttttatata
      721 gctggagctt atgaattaac aaatgcactt gtaggatctt ctttatcaaa acatggtcca
      781 atagttttag ttaatgatgg tagcaataaa agcattttaa aaaatgctaa gaagataact
      841 tctataggat atatagattc aaatatagta caacagtgtt taaatataac aaatggaatt
      901 ggagatatta atactggggt tgtaaaaaat gttaaaccta ctacaaagac tataaaagat
      961 ggaatgtata aggttggaaa agatatatca gcaggtgaat acttaattac ttcaaactca
     1021 ggaagttatg cttcttatta tgaagttaca agtgatagta caggtaatgc tgatagtata
     1081 ctttcaaatg atatttttag tgggactaga tatatcacct taaagaatgg tcaatatatt
     1141 aaaattgagg attcaacaat gacattagca aagtatgcta aagcccagaa ggccaagaat
     1201 ggtaagtttg gaaatggtat gtataaaatt ggattagaaa ttcctgctgg agaatatatt
     1261 ataatgtcaa attcttctga tgcatattat gaagttagaa atgatagctt aggtaatgca
     1321 gagggaatag ttactaatga tactttttca ggaagaagat atataacggt tgaggaaggt
     1381 caatatttaa tattaaatga ttgttattta attgaaaatg aatagatgta attgtattta
     1441 atagtaaatc atatatttta tttaaggaaa gatgttttaa gttttaattt aaaacatctt
     1501 tttattatta taataattat atgattttat attttagaaa tatagtatat ataaatcatt
     1561 agatttacta ttaaaacaca actagaaaaa taataatatc tttaaaagat ttttcttatt
     1621 tgatataatt aaggttatgt gtaatataag aaattaacta ggaggaatag acgtgagcaa
     1681 aagaaaacaa gtaacaatac ctatagagaa aatacaagaa caagataaat atatagaaca
     1741 aatacacaga caaaatgaag aatacttcaa cagaactgga aagagaaaac tcgtgtttac
     1801 tcaaaccttt gggtgtcaaa tgaatgaaca tgattcagaa aaattatgtt cgatgttaga
     1861 agagatggga taccaaatgt caatgatggt tgaagaaagt gatttaataa tatataacac
     1921 ctgtgcagtt agagaaaatg cagagctaaa agtgtatggt aacttaggac aattaaaaca
     1981 tctaaaagga aaaaatccag atatgaaaat agctgtatgt ggatgtatga tgcaacaacc
     2041 acatgttgta gaggaattaa gaaaaaaata taaacatgta gaccttatat ttggaacaca
     2101 taatctatat aaatttccgc aattacttac agaatcaata aattctgaca aaatgttagt
     2161 ggatgtatgg gatgtggatg gagaagtaat agaaggtctt agaagtaata gaaaatttga
     2221 attaaaagca tttgtaaata taatgtatgg atgcaataat ttctgcacat attgtatagt
     2281 cccatacaca agaggaagag aaagaagtag aactcctgaa gatataatta acgagataaa
     2341 agagcttgtt gcaaatggaa cgaaagaaat cactttactt ggacaaaatg tagattctta
     2401 tggtaagaca cttgaaaatc cagtttcatt ctcagaactt ttaagaaaag tgaatgatat
     2461 agaaggaatt gaaagggttc gatttatgac atctcatcca aaagatatat ctgatgaagt
     2521 aatatacgca attagagatt gtgataaggt gtgtgagttt ttacatctac caatacaatg
     2581 tggaagcagt agccttttga aaaaaatgaa tagacattat acaaaggaat attatttaga
     2641 aataatagaa aaagctaaaa aggaagttcc aggtattgct ttttctacag acttaatgat
     2701 tggtttccca ggagaaacag aagaagattt attggacact ttagatgtgg ttgaaaaagt
     2761 tagatatgat tcagctttta catttattta ttctaaaaga cagggaactc ctgctgctaa
     2821 aatggaaaat caaataccag aagatataaa gcatgataga tttaataggg tgttagaagc
     2881 tgtaaatagg atttctgcag aaataaatga tggatataaa gatagaatag ttgaagttct
     2941 agttgaagga agaagtaaaa ataatgaaaa taagtttgct ggtagaacta gacaaaataa
     3001 actcgtaaat ttcgaaggtg gaaatgatga tttaattggt aaattagtta tggtcaaaat
     3061 tactgaacct agaacttttt cattaaatgg gatattagta aataattaat attgtacaaa
     3121 ataataaaat tatgtaaact aatcgaaata gatgtactat agaacaaatt atattaaaaa
     3181 tttttattt
//
LOCUS       DAEPLF010000298         3186 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEPLF010000298 DAEPLF010000000
VERSION     DAEPLF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.299"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(73..804)
                     /locus_tag="KR314_003435"
     CDS             complement(73..804)
                     /locus_tag="KR314_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329787.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(791..1486)
                     /locus_tag="KR314_003436"
     CDS             complement(791..1486)
                     /locus_tag="KR314_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0329788.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(1488..2279)
                     /locus_tag="KR314_003437"
     CDS             complement(1488..2279)
                     /locus_tag="KR314_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329789.1"
                     /translation="MKNKFFTTLLTGFFAIVFFFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(2289..3101)
                     /gene="modA"
                     /locus_tag="KR314_003438"
     CDS             complement(2289..3101)
                     /gene="modA"
                     /locus_tag="KR314_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0329790.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
BASE COUNT         1057 a          491 c          408 g         1230 t
ORIGIN      
        1 aataaaagta ataacttttt aaatttaaag agtacacata tatatttgtg tactccttga
       61 tgatatgatt gcttatttac tacaaaaaca gagtcttttt aaacctaaaa tatttgatgc
      121 accagctatc gtagttccat aatagtaaac tggtttgtta agtgatagaa aatttataat
      181 tgagttatta cataatgtac ttcctgtgca aatcactaaa tcagcccaag atattacatc
      241 ttcataatct cgtataccat cttcaatctt aacattatat tgaatagtat ccacaaattc
      301 aggatttaaa tctaatactc ttgtttcgaa aaagtcctta atattatcaa ttatggctgg
      361 ttgatatcct ataattgcta cttttggatt accaaattcc atttttataa actcagggaa
      421 tttttttgca catacctctg gctcgttatt tttacaatga atggttctat ccgtcttgcc
      481 taagtatttc ataactgcat ttaatgatgc tataaaaaga cttctatcat gaggattgtc
      541 atgtaaatct aatgataata tgtcttttaa agaaccttca aatgtagatg gagtagaagt
      601 aaatgcttgt cctttagctc ctttaaagtc agcttcaagc attatttctt taccaactat
      661 aattgggaaa tctttacgct tagtatttcc aattgcttct tttgaagata gagatttgga
      721 cacaatactt atcatatcca tatttaaatt atatttatcg acttgtacta atagataatc
      781 tctcaaaata tcaaatatat tcatagaaaa aagattctcc tttatttttt atttcttcat
      841 ttaaatcaaa aatatgattt atttttccat tgttcattat tcctatctta tgagcaaaaa
      901 acattgcttc agaaatatca tgagtaacat gaatcactgt tggtttgtat tttatgtaga
      961 catccttcaa aattttgctt atatgttttt ttgtaatata atccaatgaa ctcataggtt
     1021 catctaaaag taatatatca ggttcagcta ttaaggcacg tgcaaaagca actcgttgct
     1081 tttcgcctcc acttaaattt ttaggatacc tattttttat atcttctatc tgcattaatt
     1141 tagacaagtg gttagcctta agaatctgtt catctatagg tatttttctt aatttaagtg
     1201 gaaaccttat attatcttca acattcatat ttggaaataa ttcaaaattt tgatatacaa
     1261 atcctatatt tctattttct ggaggtaagt ttgatattaa attttctttg tatgatacat
     1321 ttcctttagc agatggatat cttcctgcta atgtttctag gaatagagtt ttacctgacc
     1381 cactagctcc taataaaaca aagtattcat gattattaat atttaaacta ttttcttgta
     1441 atttaaaatt aggtaaatca agagataaat tttttatttt taacatatta attcttatca
     1501 acctctctat taaaaaatat ctcaaaaagc atcaagcatg taagtgagat aaatatcaaa
     1561 attacagcta ttccgattgc aatgtctata tcaccagtag acatatttaa atatatagat
     1621 gtaggaataa tttcagtttt catacgtgta gaacctgcta tcatagctac tgcaccaaat
     1681 tcaccaagtg ctcttgacca agtcattaca gttgcagata tgattccagt ttttagattt
     1741 ggaagaatta taaatctaaa tgcttcccaa ctattacatc caagggtacg tgcaacaaat
     1801 atcatctttt cattactttc atttagtgaa gtgtgaagta ttttaatagt aagtggaaga
     1861 tttacaaata cttgtgctaa aactatacct tgctttgtaa atataaaatc aagcttaaaa
     1921 atcttataaa tggaatctcc aatccccatt cttccaaaca aaagtaaaag agcaattcct
     1981 gatactaagt gtggaagaga catagggaga tttattatct gtataatcag ttttttaaat
     2041 ggtaatttag taatatgtaa aaaatatgct actggaatag ctaataaaag gcatactata
     2101 gtagatatta aagatgtttt aatactaagt gttattgcat actgtacttc cacggatttt
     2161 aagcatatag gaacataact tatcccttta atgattaaca taagaagtgg tgatacaata
     2221 aaaaaaaaga ctatagcaaa aaatccagta agtagtgtag taaaaaattt atttttcaag
     2281 tttaatcact acttaacagg ttcaaaacca tattttttaa atatctgttt agcttcatct
     2341 gactctatat aatttataaa tttattagct aaatcttttt tatcactata gtttacgatt
     2401 cctgctgata tgagttggtc tatattttta tcttgggcaa tttctataac ttctacatct
     2461 tcattgccaa agaccgaatc ttttgtggct attgtagcat cagctttact tgatgttaac
     2521 tgtacaatca tttcatttac agttgcagct gtagagacaa cattatcatt tatagatttt
     2581 aaattatttt tttcaagtat tttttgtgta gtcttaccta tagcattaga ctctttgtct
     2641 cccaaaataa gtttaactcc atctttattt aagtcttcca atttttgaat attagctgga
     2701 tttcctttag gtacaactat tgcaggtgta tgatgtgcaa ccgttttatg ttcacctaca
     2761 agttcttttt caaaggcctt tgaataaatt ggttcagaac caacaataaa tacgtctcct
     2821 ttgtgacttg tctcaatttg agctattagc tgtgcagaac ctgcatagct atattcaact
     2881 ttgacattat tttctttctc aaattttttt gctatttcat ccattggttt ctttaaacct
     2941 gcccctgaat agacaaaaag agtctctttt tcttttgaat catcagctgt tgtcacttta
     3001 gtattagatt catcattagc tttgttttct ttagaattac atgcagtaat acttaatgaa
     3061 ataatcatag ctaaagctag tactgaaagt tttttaaaca taagatattc ccccttagta
     3121 aattagtgta aattcttttt tagtatatca tactaagaaa acgatgtcta agtttttgtt
     3181 atgttt
//
LOCUS       DAEPLF010000299         3146 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEPLF010000299 DAEPLF010000000
VERSION     DAEPLF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.300"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..310)
                     /locus_tag="KR314_003439"
     CDS             complement(<1..310)
                     /locus_tag="KR314_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329791.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     I"
     gene            585..863
                     /locus_tag="KR314_003440"
     CDS             585..863
                     /locus_tag="KR314_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329792.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(992..1738)
                     /locus_tag="KR314_003441"
     CDS             complement(992..1738)
                     /locus_tag="KR314_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329793.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLDFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(1867..2511)
                     /gene="trmB"
                     /locus_tag="KR314_003442"
     CDS             complement(1867..2511)
                     /gene="trmB"
                     /locus_tag="KR314_003442"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBH0329794.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(2649..2969)
                     /locus_tag="KR314_003443"
     CDS             complement(2649..2969)
                     /locus_tag="KR314_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBH0329795.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
BASE COUNT         1094 a          495 c          330 g         1227 t
ORIGIN      
        1 ctataaaacc aaaaactact gtaataagta ttaatctttt atttaaagta aaaaaaggta
       61 tattaaaaat acttttgtcg gatgtttctg gagtataaga atatataact ccaaaaatca
      121 aagtaagcat acaaagtgga agtagtgtat aacttgaaat tattcccaga aatcctatac
      181 tttcaaaata tttgctagca ctatttgtat aaacaaacat gtatccattt aaatatgcgc
      241 atatacatga tataatgcca aaagttcccc ctaaggattg agtgtaccaa ttaaaaaatt
      301 tagattccat atacctcacc tcctaaaact gtatatataa tgatatgcat ttaaatagta
      361 aaatatatct ttatatgttt ctgaataaaa ttttgttaat ttcattaatt tatgtttaag
      421 aaatttgaag tatagtgtag tatatattaa tgtagatgaa gttttatagt acttatttca
      481 tagtgcttaa tcaagttatc ttattatgga ttcaaaagtt gtttgtaggg ttttatattt
      541 tgttgagctg aaaaatttat tataaatgaa gggatgtttt cgctttggta gatacattag
      601 aatttggtct taaaatttta ttttttatat tatcaataat ttggatgggt aaaataatga
      661 ttttaagaac tgataagcaa atagtaatca atcctttgct tattggtata tctgcagtcc
      721 ttgtgatgtt gcatacctca cagagtaaca ttgaattttt tggattagat gtccaatata
      781 taagaatagt tttgtatata atatattcac tgataatttt aataggaata tgggctacta
      841 acagaagaaa tggaattttt tagataatct atttctataa aatttaaagg ttgctagtaa
      901 caaagcacta atattagcgt tctgttacta acaaccttta ttttgattat acacttaaat
      961 tgaaaataga atgattgaat aagttgacat attattttac attgacagtg attagaactt
     1021 cttgagatgt atttaaatca tttttgtcgc ttagtataac ttttaccaca gcttgacctg
     1081 ttttattacc tttaattatc atatcttcac atgaaagtac actcttatca cttacagata
     1141 caataaagtc taaatctttt ggaagattat cagtaacttc ccagtattta taataactat
     1201 caattttctt cgtaattatt tttctcaaat caatactttc accttttgct atatcaacat
     1261 tatgatattt agagtccagt ttatactgat tatcttttgt atcaataaaa tttaaatcgc
     1321 aatttacatc cacaccatta tgagaaagag aatttactac atcgtcatca atcaaataat
     1381 tattggcaag atttattgaa tccatatctt ttagacttaa agcttcctta ggaacctctt
     1441 ttatattatt attccaaaaa ataaaattgt ctacattttc aagatatgat attcctttta
     1501 aatttgaaat gctctcacca gttgcataaa attctccatc taaagccttt atattgctta
     1561 tagtcatctc atctgggttt atatatcttt ttacaactgc tcttaaattt gcatcaggaa
     1621 aaatatcttt aagttgattt tcgctcaaaa ccttatcatt tgaatttatt ggacttaaag
     1681 ctgctgcaga aataataaat ggtattaaat tagctaaatt catagtaccc tcccacataa
     1741 attttttatt ataataattt aatttctatg agagggttct gtttccttgt tatatatacc
     1801 tactttcgta cactttatta atataaatta aaataaattt ctactattaa acttataaac
     1861 atattactag ttacgttttt tagcttcaca tctaaatatt cgcatacctt gtgacataaa
     1921 tttgtcttca tactctgttg taacattatt ttcatactca ctatttccta aatcaagaga
     1981 aatattcttt aatagccaat tatttgcagc aatttcattt aaactaaact cgaataattt
     2041 ttcattatct gttttaaagt gaatttcgcc atcatcttcc aaaaccctat tgtacatttc
     2101 taaaaatcca gaatgagtga gtcttctttt actccatcta tttttaggcc atgggtcaca
     2161 gaaatttata taaattctcg ataattcttt agcttcaaag taatccaaaa tattgcttac
     2221 atctccccat aaaaatagta tattattcaa attagaagca tctgcttttt caacagcttt
     2281 taaaagaact tcttccttaa gttccatggc aatataattt atgtcagggt tctgcttagc
     2341 taaagttgtt ataaacttac cttttccagt cccaaattca acatgtattg gattatcatt
     2401 tctaaacttt aaattccact tgccttttaa tttgtctata tcgtctctta aaacatattt
     2461 tgtataactt aaaagctttt catctgcacc tttttttcgt cttcttctca caataattct
     2521 ccttacatat aaaaaattag taaaatacat ttttattata cattatctca ccaaaaaata
     2581 aaaagaactc tcttaaaata gaaatttatt tctataaaga gagttctaaa cttactaaaa
     2641 acttaatctt agtggcaccc tgaacaacca gagcatcctc ctccaccttg atctttcata
     2701 ttttcaggaa taactctaaa tccaaatcct gtatcttcta ttattatatc tccatactga
     2761 ctatattcat cagagctcat tacaaattgt aattctccaa cttcataagt taagtcatct
     2821 tcttttttct catctaaagc taatccaaaa gaaggacctc cacaaccaac acctgcaaag
     2881 tatactctta tattattagg tttatcttga ttgtctttta agatactttt taaagtatca
     2941 atagctgttt ctggtaaagt aattttcatc aaatcattcc ctttcaaata ttttattaaa
     3001 acaatatcta ttatataccc atacaaggta cataatcaat tagtttttgc atattttact
     3061 aaaacttcta cctaatatta cctaaggctt taaaatattt tatataatat tttattacct
     3121 taaataaata ccctatcata aactta
//
LOCUS       DAEPLF010000300         3108 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.301, whole genome shotgun sequence.
ACCESSION   DAEPLF010000300 DAEPLF010000000
VERSION     DAEPLF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.301"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..617
                     /locus_tag="KR314_003444"
     CDS             <1..617
                     /locus_tag="KR314_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329796.1"
                     /translation="TLFSKVTFRYEKLKFKDNAELEKIKMFKYKNGYIPNKLNLSFGY
                     GFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDGTKKLEISIEKVVKFKRRKKRN
                     YVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIFEDEDVIINKKVLKFPKSWTKN
                     MQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVIILYYIYNH"
     gene            complement(945..1712)
                     /locus_tag="KR314_003445"
     CDS             complement(945..1712)
                     /locus_tag="KR314_003445"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBH0329797.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKVAVMPQNERDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            2518..>3108
                     /locus_tag="KR314_003446"
     CDS             2518..>3108
                     /locus_tag="KR314_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBH0329798.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSN"
BASE COUNT         1223 a          419 c          361 g         1105 t
ORIGIN      
        1 atactctttt tagcaaagta acttttaggt atgaaaagtt gaaatttaaa gataatgctg
       61 aattggaaaa aattaagatg tttaaatata aaaatggcta catccctaat aaattaaacc
      121 tttcttttgg atatggattc tcttcttata aaaagcaaat aattagagaa actgtagata
      181 ctttaagatt gacagaaatt ttttcaagcg agaacataga agatattaaa tttataaaag
      241 atggtacaaa aaaattagaa attagcatag agaaagttgt gaaatttaaa cgtcgaaaaa
      301 aaagaaatta tgtttgttgc tattgccctg atatgtatag agatataaaa ctcgacaaag
      361 aatctatcaa taagatatac aacagaaaaa taaaaataga aagagaagtt aatatttttg
      421 aagatgagga tgttataata aacaagaaag tattgaagtt tccaaagtct tggacaaaga
      481 atatgcaaaa atattggtta agtgaaaata agtatcccat acattctact gtaattgatg
      541 atgatagata taaatgttgt aatgtacaat atacaaaaaa tagagtgata atattatatt
      601 acatatataa ccattaatat acataattac aaaaaaataa tgacaaatat acataataca
      661 aatatatctt ctactttaga taaaacagtt attatgaaaa cttactatct ataaataata
      721 gaaatgctag tataccatct ataatctaat gtctagtagg ttttaatggc atattttaaa
      781 tatgccatag ttgatttata agattttcta tatcactttc aaaatacaca ctaaatagtt
      841 gcttttcaga tataattgcc caatttaaat tttactatct gaaaagtttt ttatatttaa
      901 tttatatctt caaaaatacc gtctaatatt tctatataat taatttaatt aatactctta
      961 tgtaatagat taagtactga atttaactct ttaaatgata tttgccctgg tgctgaaaca
     1021 ctttttgcag ctccaaaagt caatgcagaa ccaaatattt caccacacaa tctacttata
     1081 acacccattc ctgacattga cattgttatt attggtctat ctgcataaat tttaaacatt
     1141 tcatttgtag cttcaagtag cactaacaca tctctttcat tttgcggcat aacagctact
     1201 ttgggtaaat ctgcgcccaa ttcctgcatt ctacataaac gagatacaat ctcttctttt
     1261 tttggagttt tattaaaatc atgattagat ataattactt tcacttcttt tttatgagca
     1321 aaattaacta cttcatctat gacctcatct cccataaata actctacatc tattaaatct
     1381 actaaacctg tattagaaat ttctttattc aaagtagtat aataatctct tgatattaat
     1441 ttttctcccc cttctactac acttctaaat gtgaataata aagggatatc atgtatataa
     1501 cttcttaatt catagagaac ttccttaact tctttaatat tttctacatt ctcaaaaaaa
     1561 tcaactcgcc actctataat atccaaacat gcatctttta actctttagc ctcttttata
     1621 atatcttttt tatttttccc aataattggt acacaaattt taggtctacc ttctccaata
     1681 gtaatatttt ttacttgaac ttttcttttc atattctcct ccataatcag aaattctata
     1741 ggactttaaa ttctattttc tgcataattt aattatacat taaaaatact atacattata
     1801 aaaatactaa tttaaaaata atttttattc taattcttaa aacaatccca catattttag
     1861 aaaaggtact ctataatata caaacacttc ttttttacct aaatttattt taacattcta
     1921 taattttcac aatattcttt aaattttact catataaaca atattattct ggatgctaat
     1981 agaattaatc tataaatcat agtataatta tgcagaaata actcgaattt cactacttat
     2041 aaagagattg aatattaatg aatctaaatc atttgcatta cttcagagtt ttgactaaag
     2101 ttgaacacca tacaaaagta gctactcaat tgtttatacc ccaaccaagc ttaagtaatg
     2161 ttatatagat tcttgaaaaa gaatttggta tctttttatt tgaaaaacaa ggtagaaatg
     2221 tttccctcac taaatacgga agtttttttc taacctatgt tgataaagca ttaggttaat
     2281 tagaaaaagt ttacatataa aatatattaa attgttgcaa gttttattgt tttgatatac
     2341 aatcaactaa acatataatc tttataaaat actatacagt taaaagtctg ttttaaaaaa
     2401 aaatgtataa attataaatc aaaatataga tactatcaag gagtgattct gtgaagagat
     2461 gtattgtagt agtggaatag tcaaaaacat aatatatatt aaaggagaga tttaaatatg
     2521 actattccaa attcaaataa ggtatatcca agaagtaatg attatcaaac tgtatatctt
     2581 aaaaatgtaa taacaaggga aaatatagaa gttggagatt acacaatata taatgacttt
     2641 tataatgacc caagagattt tgagaaaaac aatgtattat atcattaccc tataaacaat
     2701 gataaattaa taattggtaa gttttgctca atagcatgta atgcaaaatt tctaatgact
     2761 agtggaaatc actcaatgaa atcaaaatcc acatatactt ttcccatctt ttatgaagaa
     2821 tggaaactcg atatcaacca tataacggac gcttgggaca ataagggtaa cattataatt
     2881 ggaaatgatg tgtggatagg ttatgattct ataattatgt caggtgtaaa tattggtgat
     2941 ggtgcaatta ttggaacaag agcagtagtt actaatgacg tctcaccgta ttctattgtt
     3001 ggaggagtac ctgcaaaaat tataaaaaag agatttaata atgatacaat ttcaaaatta
     3061 ttagaaatta aatggtggga ttggccatat aaaaaaattc aatcaaac
//
LOCUS       DAEPLF010000301         3089 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.302, whole genome shotgun sequence.
ACCESSION   DAEPLF010000301 DAEPLF010000000
VERSION     DAEPLF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.302"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(132..1031)
                     /locus_tag="KR314_003447"
     CDS             complement(132..1031)
                     /locus_tag="KR314_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBH0329799.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(1058..1696)
                     /locus_tag="KR314_003448"
     CDS             complement(1058..1696)
                     /locus_tag="KR314_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBH0329800.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KVGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(1860..2876)
                     /gene="gap"
                     /locus_tag="KR314_003449"
     CDS             complement(1860..2876)
                     /gene="gap"
                     /locus_tag="KR314_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBH0329801.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYSDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNQKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
BASE COUNT          914 a          550 c          311 g         1314 t
ORIGIN      
        1 aagttctttt aaaccaggta aataatctta caatcaaaaa atgaagactt aaaatataaa
       61 ttacttctac tataaataaa cttagttaga agtaattttt tatttgagtc ttcatcattt
      121 actttaaatg attaactttt ttcactacag attcccataa cttctattcc atcttttatg
      181 cattctatgt taagaggaaa atctggtcct ttttctccgt caatatctgt tataagtgaa
      241 tcttcacatt ctatatgcac ctttttagtt ttaaagtata gtatttcatt tccattatat
      301 tgatctaaat aatcacctct aagcacactt atcaaaactg gtattgattt tggtattggc
      361 attcctttaa aaataattac atccaaatat ccatcatcta tttcagcctt atatgctaga
      421 tttatatttc ctgctgtttt gccattaaaa atcaacataa gatacatatc tccatcataa
      481 tacatttcct cagaagatac ttttatctta aatttcctca tatacaatgc ttcttctatt
      541 ccctttatat aataagaaat tcttccaaat gaatttttta agtctgtgtt tatcctttgt
      601 gatacatctg taaacatacc agcacttgca acatttataa agtacttatt attgactttc
      661 cctatgtcta ttttctttgg ttttgaattt atgatttcat ttatagcttc ttttatatca
      721 aatgaaattt gaagagcatt tgaaaaatca tttgcagttc ctgtaggcaa aattccaatt
      781 ggaattttta tgtctaattc tttcattgca tttactacta aatctacagt accatctcca
      841 ccagctatca atatgtgttc ataagaacta tcaacttcct taaatgcatt ctttacaggt
      901 tctttaatag ttaatctata gggtatcaat aaataatttt tttcttgata taattctatg
      961 atagtgtcta aatttgataa tatctttttt tcacctgaat taggattgta tataagtttt
     1021 acttttttca ttatttctcc ttactcattt tattctttta gtatccaaaa tcttcattta
     1081 aaaaaatcac atgtattctt tctggtgtta attgtctttt tataagtgta taactacagc
     1141 atattcttcc tggacttgaa cagtctatac atataccagt agtcttacat ccagtatttg
     1201 tacttaatct tttacaattg gctggagcag ctattgtttt tgttcttttt atagcttcat
     1261 ctacatcttt aactatttta tttactcctg ctataactat tactttatca ggtccatata
     1321 tcatagcagc aactctattc ccattcccat ctaaattgta aagttctcca tcctctgtta
     1381 tcgcattagt acttgtaaaa taagcatctg cagaaaatga ctttctatat atttctttca
     1441 tttcttctgg tttgatatct tttgcatatc tatcaagaaa attatatttt tctcttataa
     1501 aatcaatcac tcctgtttca aataaggtca tagaacctcc acatcctact agagaaccta
     1561 ctttaactat ttcttcaatt tttttaagaa gctcttcttt gttacttact aaataccctt
     1621 tcatattatt gttttccaga gattttattg ttttttctat tcttttttca ttaagccaat
     1681 ttaaattttg atccatttta acttccccct tatttttaat atgtacttaa ttatttataa
     1741 actttattgt ctttataagt ttattttagt acatctaaaa aaaatacaat ataaaactta
     1801 aatagtttta tataattttt tcaaaaaatt tttttaaaat ttgctttaag aaatatatat
     1861 tattttaatt tttcagctac atattttact aagtcaacag ttcttgaaga atatccccat
     1921 tcattatcat accaagaaac taccttaacc atattgtctt ctataaccat tgtggataga
     1981 gcgtctataa tagatgatct tgaatctcct ttataatcta tagaaacaag tggcttgtca
     2041 caatatccta aaacaccttt taattctcct tcagcagcat cttttaacac agagttaact
     2101 tgctcagatg taacgttttc tttaagttcg cacactaaat caacaagtga aactgttggt
     2161 gttggtactc taagtgaaaa accatttaat tttccttcta attgtggtag tactcttgac
     2221 acagcttttg ctgctcctgt agtggtaggt attatagatt cagccgctgc tcttgctctt
     2281 ctcaaatctt tatggctttt atctaatatt ctttgatcgt tagtatatga atgtactgta
     2341 gtcattaacc cttttactat accaaatttt tcgtccaata ctttagcaaa aggagctaga
     2401 caatttgtag tacaagatgc atttgaaatt atattgtgct tttgattatc atattctttc
     2461 tcattaactc cgataactat agttatatct tcattttttc ctggagctgt tataattact
     2521 ttttttgctc ctgctttaat atgtttttct gccttttctc tttgtgtaaa taatcctgtt
     2581 gattctatta ctatatcaac ccccagttct ttccatggta attcttctgg gtcactatat
     2641 cttaatattt ttatctcttt agagtttact actagagtgt cttcatcctt tgcctctact
     2701 tcacctctaa aagttccata gcatgaatca tatgtaaata aatgtgccaa agattctgtt
     2761 gttgctcttg catttatggc tacaatttct atattatctc ctaattcttc ttgagctatt
     2821 cttaatacag ctcttcctat tcttccaaat ccgttcattc ctaccttaat tttcattatg
     2881 aaatgtctcc ctccattact atgtttttac atgattattg tacactaaat aaatcatttg
     2941 ttcaatactt tttcttgatt aaataatcaa attccatgtt tttttattat caaaagacaa
     3001 ttagtatatc atttttcttt atttcacccc tcatttagta tgtacttttt agtgtaaata
     3061 agctgaaatc atttatttta ctcaatgtc
//
LOCUS       DAEPLF010000302         3062 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.303, whole genome shotgun sequence.
ACCESSION   DAEPLF010000302 DAEPLF010000000
VERSION     DAEPLF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.303"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            230..1309
                     /locus_tag="KR314_003450"
     CDS             230..1309
                     /locus_tag="KR314_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBH0329802.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDEINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYMKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRRH
                     YPGIHSITIIINGVEKGKLDFELGV"
     gene            1633..2001
                     /locus_tag="KR314_003451"
     CDS             1633..2001
                     /locus_tag="KR314_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBH0329803.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2118..2921
                     /locus_tag="KR314_003452"
     CDS             2118..2921
                     /locus_tag="KR314_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0329804.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFMPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPSELNQIMDIFIKQLNI"
BASE COUNT         1149 a          361 c          579 g          973 t
ORIGIN      
        1 agtaaagtat aaaattctat tactataata aaagatagaa ctagaagtct ttatgaaagt
       61 gggaaaagta tttatataaa taaatttata tggtttatag tttaacagta ggaaatatcg
      121 tatgagtgag aatatcgtat tttacataaa gatttagcaa tatgacacac tgttgtcgtg
      181 ttctgtagga tatgattaat aaatcagata tgaaaggaga gtgagtaata tgccagaatt
      241 actaaaaaat atgtataatc gtgagtcact ttatgaagtt gcagtagcta tccaatcagt
      301 ttacaactca tttaaggtag acgagttcat aaaatctaca atggatgaga catggaataa
      361 cttagaatta aaagccagat gtcgaaagat aagtatgagt ttaggaatgt atttaccaga
      421 agactataag gaagctcttt ccattcttga aaaatcagtt acaggttttt attttgcttt
      481 tttctttcca gattttgttg aagtttatgg gcaggacgaa ataaattggg atttatctat
      541 aagtgcatta gaaagaaata ctgagtattg gtcttcagaa tttgctgtaa gagcatttat
      601 catcaaggat gaagagcgta tgatggcaca gatgcgtaaa tggtctaagc ataagagcga
      661 acatgtacga agacttgcaa gtgaaggttg tcgtccccag ttgccttggg gacaagcaat
      721 atccaaattt aaaaaagacc ctacacctgt actgcctata ttggagcaac ttaaaacaga
      781 tacatcaacg tatgtacaaa agagtgttgc taataatcta aatgatatct caaaaacaca
      841 tccagattta gtcataagca ttgcaaagga ttggtacggt aaaaataaat ctactaactg
      901 gattgtaaag catggttgta gaactctact aaaaaaaggc aatcgtgatg tattagccct
      961 ttttggatat gatgatacta cttctataaa tcttcaggac tttactttag aaacaacgtc
     1021 tatttctatt ggagaggatt taactttttc atttaatatt ttagcaaaaa aagcgacaaa
     1081 aacaaggctc gagtacggaa ttgattatat gaaatctaat ggaaaaagga ataggaagat
     1141 atttaaaata tcggaggttt cattaaaaga aaatgagaaa aagtcatata tgaaaaaaca
     1201 ttcatttgca gatgtaagtg taaggagaca ttatccaggt atccactcga ttactattat
     1261 tattaatggg gtagaaaaag gcaaacttga ttttgaatta ggtgtataat tttactaaca
     1321 gcataaccta tatcagatac tttgctaaac ctatatcgta tactctaata aatctatatt
     1381 aggtagttta ctacttgttt actgaattta aatacaaaga aatacaggtg ttaatccctg
     1441 cccattaaag gaatatgagc ataacacaaa agtagaactt atcaattctg ttacgggtat
     1501 agtagtaaat ccatagaatc acaattagaa gatgaagtgt aaaaaataag ttagtgttga
     1561 ttttagttgc tagtgatatt gaatagttat acaatagcca tataaattag aatttgtaga
     1621 ggagaaatgt aaatggatgt atatagtttt tggaatgaca ctttggcaca agatgctgtt
     1681 aagataagaa aatattttca tgaagatgct tatattaatt ggcatgatac aaatgagcat
     1741 tttactttag aagaatttat tattgcaaat tgtgagtatc ctgataaatg gaatggtaaa
     1801 gtagaaaggg tgctagaatt tggaaattta gtaattacag tgacacatgt ttatgcaata
     1861 aataaaccac tatcatttca tgtcgtttca tttatcagcc ttaaagatga taagattatt
     1921 tctattgatg aatattgggg agaggatgat gatgtgccac agtggagatt agataaacat
     1981 attggaaagc caataaaata gtgtttgttc tgttttataa ctgaaatttt gataacattt
     2041 gatattttga tttgtcattg gtcaacatag tcgttagtgt aataatttaa aaaagtagaa
     2101 tttggagagt gagcactatg gaagaaaaat acatcttatc tgaccgaggt aaagtatatt
     2161 attggataaa agaaagtaat agtgtgtttg caaagaactt aatttttttg ccaggactaa
     2221 cagcaaatca tcatcttttt gatttccaga tacagtactt ttcaaaaaat cataatgtat
     2281 tggtatggga cgctcctgct catggaaaat caagacctta cttagatttc tcatattcta
     2341 acttggtaga agaattgaaa gtaattttag ataaacaaaa tatgaaaaag gttgtactta
     2401 tagggcaatc ggcaggggga tttgtggcac agtcctttat tgtaaaatat ccaaatatgg
     2461 tagaaggatt aatgatgatt ggaacttgtc catatggaac tgactattat agtaagtcag
     2521 atattttctg gttaaaacag actgaatgga tgttaaagtt attcccagat aaattgttgc
     2581 gaaacataat atctaagatg tgtggagcta cgtctgaagg cagaaggagt atgatgaaaa
     2641 tgttagaagg ctacgaaaaa aaagaactgt gtcatctcat gtatctggga tttgctgggt
     2701 tcatgccaga aattcaagac ttaaatatac attgtccagt ttgtttggtg gttggagata
     2761 aagacaagac gggtaaagtg agaaaatata atgagcagtg gcacaataag actgggtatc
     2821 cattgcacat tataaaaaat gcttcacata atgtaaatgt cgataaacct agtgaactaa
     2881 atcagattat ggatatattt attaagcaat taaatatata aaaactacat actcattagg
     2941 taaattatgt agtatatgtg tttacatatg gttatagaaa taaatatgta ataaactact
     3001 taacacttaa aaagtggatt tgtccagagc ataatacaaa gcatgatata taaattttta
     3061 ag
//
LOCUS       DAEPLF010000303         3037 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.304, whole genome shotgun sequence.
ACCESSION   DAEPLF010000303 DAEPLF010000000
VERSION     DAEPLF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.304"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            268..534
                     /gene="rpsT"
                     /locus_tag="KR314_003453"
     CDS             268..534
                     /gene="rpsT"
                     /locus_tag="KR314_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBH0329805.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            complement(721..1764)
                     /gene="holA"
                     /locus_tag="KR314_003454"
     CDS             complement(721..1764)
                     /gene="holA"
                     /locus_tag="KR314_003454"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBH0329806.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(1718..3019)
                     /locus_tag="KR314_003455"
     CDS             complement(1718..3019)
                     /locus_tag="KR314_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBH0329807.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIESPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
BASE COUNT         1113 a          436 c          282 g         1206 t
ORIGIN      
        1 gtatgttaat agtttgtatt atctcaaaat atttgttaca aaaataaaat attaatttat
       61 atatgtcaaa atatttaaaa cacaaaatat accaatttaa aaacaagata ttgcaaatta
      121 tttatacaca atttacagtc taaaaaatca aaataaaaaa cattattaaa atatataaat
      181 aataatcctt gatttttaga tttatttctg ttaaaatcta atatgttgac gatgaatatg
      241 ataaaaaaat acagggaggt gaacacggtg gcaaatataa aatcagctaa gaaaagaata
      301 agtgttatcg aaaagaaaac tgctttaaat agagttagaa aatctcaaat aaaaactgca
      361 ataagaagat tcgaagatgc tgttgcagct ggaaacagag aggatgctgt tgcaaagttc
      421 caatacgctc aaaaaagaat ataccaagta gcttctaaag gaactataca taagaatgct
      481 gcagctagaa aagtagctaa attagctcaa aaattaaacg gtatgaacgc ttaattacca
      541 gtttatattt cttatatata ataaccccta tgtaggggtt ttttattttg taaatttata
      601 caataaaaaa taaatagtta gacatattat agttgattgt atatcaaact taatttagct
      661 tacatctact ctgaactata acatatcttg ctatttattt tttattaata tatagatttt
      721 ttaaattgct tctcttttac agtatctact aaccaacatt tcaactgcta aagtatctct
      781 tataagacct gtttttattt tataatcact ttctaatata tagtttaata tttccacaat
      841 tatatcatca gaaaaatttt tagtttgctt taatgctttt cctacaacaa attgatgcat
      901 ttttaattta tcagctataa gttttgttga atatccatca agttgtaact gtcttacttg
      961 cataattatt ttaaattgtc ttgctatcat agaaaatata cctaatacag attcgccttc
     1021 ttgtatcata tcatttaata ttttcattgc attagaagca ttttgttctc caatataatc
     1081 gattaattta aatatatcat tctcaacttt tttttgagat aatttatcaa ttacgtcatt
     1141 ggttacatta ttccctttac caacaaagga acttatttta tttatttcat tttctaaatc
     1201 agataatgtc ttttcactgc tcttatctct gtatccttct tgttcaataa aatacatgat
     1261 ttgagaatta tctattataa catcatttaa agcaaaagat ttttttatcc atttaaataa
     1321 atccatatca gaaagcttat cacaatcaaa aacaattcca ttgtttccta ttttcttaac
     1381 taatgatttt cttttgtcta catctccata tacaacaaaa actatagttg tagtgtctgg
     1441 aatattatct aaatgctcta ttaagtattt ctcatcacta tctgtaaaat tctttttctt
     1501 tccttttaat agttcgaagt cttttattat tactatcttt ctatcatcca taaatggaag
     1561 tgtttctata gagcttataa gctgatttaa gactatttcc ttcccatcta ttatatctaa
     1621 attaaaatca agcattcctt cgtttagact atctttaaat gcttttattg cattctcaat
     1681 taaataatac tctcttccat aaaaaagata cattttttca aagtttttct cttttacatt
     1741 ccttataata tccttgtaat tcattctctt gctcctttat ctcttttcct tctatgtaaa
     1801 atatgtaaaa aaacactcct atgtaataaa taacaacaat ataaaattta ggactttcta
     1861 tattaatgta tgcaaaactt aaattgctga ttttttctag taaaaaaaat atgctttcta
     1921 tcaatgtact attaaaaaaa gatataattt tagctattac tacacttact ttaaatacga
     1981 ttaaacttgc aatacttaaa tacattatta ctcccacaaa tggtactata attaaatttc
     2041 ctataattga aagcaatggt atacctttaa atgtataata aattataggt aaagttaata
     2101 tatttgatga tattgtcaat gataccatac ttaattttat aatactattt attttattat
     2161 agaatattaa tatagaaagt gtagctaaaa aagacaattg aaaacttaca ttatatataa
     2221 cataggggtt attcataatt aaaattatcc caattagaga caatgctgat attccatctt
     2281 tctttctatc aataaatatt gataaataaa aaatcataac aaatgctata gacctaatta
     2341 tggatggaga cgcacctacc attatacaat ataaagttat aaaaatcgac agtattaata
     2401 atttatacaa tttatttata cctcttaata gaaaggatat aattacacat aatattcctg
     2461 tatgaagacc tgatatagat attacatgac tagttccagt tctagtaaaa atttcttttt
     2521 gttcctcagt caaattttca acttctgcta atagtataga atttataaaa tctgagtttt
     2581 tcttatataa gtacctataa gttttactaa tataaaactt tatttcccct attaaagagt
     2641 aaagtttatt tttatcaata attttatagt tatttatata cactatacct ttatatccag
     2701 tactttttat ataccttcca taatcaaaat catcatactt cattttatct aaacttttga
     2761 atttacctgt aatattaact ttctgaccaa tcttcaaatt tttatttttt gtataatcat
     2821 ttattagaaa aacccctacc ttgtactgat tgtatctttt cttttcaatt tttcctttga
     2881 caaccccttg tatagttatt atcttatcat taagttcttc gtcgtttttt gtgttatttg
     2941 taatgataaa tgatatagct accacaaaca ttaatataat taataatggt cttcttattt
     3001 gaattataat cactcccata taaaagtata ttattta
//
LOCUS       DAEPLF010000304         3001 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.305, whole genome shotgun sequence.
ACCESSION   DAEPLF010000304 DAEPLF010000000
VERSION     DAEPLF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.305"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            332..997
                     /locus_tag="KR314_003456"
     CDS             332..997
                     /locus_tag="KR314_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBH0329808.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            1019..1951
                     /locus_tag="KR314_003457"
     CDS             1019..1951
                     /locus_tag="KR314_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBH0329809.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            1972..2613
                     /gene="pcp"
                     /locus_tag="KR314_003458"
     CDS             1972..2613
                     /gene="pcp"
                     /locus_tag="KR314_003458"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBH0329810.1"
                     /translation="MKILLTGFDPFGGELINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIK
                     GGFIHIPYTTSQVINKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(2732..>3001)
                     /locus_tag="KR314_003459"
     CDS             complement(2732..>3001)
                     /locus_tag="KR314_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329811.1"
                     /translation="LISGKYKMIILYCLVEFEVVRYNALKRYINTISYKTLSLSLKEL
                     EADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMCEWGERNRP"
BASE COUNT         1118 a          417 c          456 g         1010 t
ORIGIN      
        1 atcatttcaa aagagaaaaa tacacttgtc tctatgtttg acacattctc tttttataat
       61 ataatatcta atttaaaacc tatttcaaat attgacatat acttaaacca tagacttaca
      121 aaatcaattc atattagctt aaaatataat ctttgttact caaattccta tacacaacca
      181 ttatttactt tgatacttta aaacatcttc cctatttttt attaaatttt ataataatag
      241 tcctattaac gcaaatacta taaaaagagt ttaactttat ccattaaata aatttaaatt
      301 aaaacatata gaaatggaag gagaacaatt tatgataaaa ctaatcggta tattgatagt
      361 tgtaattgga tttatactta aaattgacac attatttact gtattattag caggagtggc
      421 aacaggaatt gttgcaggat tagattttaa tcaaattttt acaattttag gagattcttt
      481 tgtctcaaac agaggtgtat cattattcat attaacactg ccagtaatcg gtgtattaga
      541 aagatatggt ttaaaacaaa gagctgtttc tttaatagaa aaattaaaaa gacttacaac
      601 aggaaaagta cttactatat acatgatagc tagacaaata gctggtgctc tatcaataag
      661 aatgagtgga catcctcaat ttgtaagacc acttgttaat cctatggcac aagctgctgg
      721 tttaagtaat agtgatgaac taaaagaatc ggatgaagaa gccataaagg cattatctgc
      781 tgcatcagaa aactatggaa atttttatgg tcaaaactta tttgcaggta gtagtggagt
      841 attacttata gcatctactc taactcaatt tggatacaat gtaactggac ttaatattgt
      901 aaaggctagt actattatgg cagtaatagc acttgtagtg gctacaatcc agtttacctt
      961 atatgataaa aaattaaaca aaagtcataa aaaatagttt tatttaggag gaataaaaat
     1021 gcaaaatttt ataaacatat cattggaaat cttttacgca ctaatgggat ttttaatgat
     1081 tgttatagca tataagtctt ttactaccat aaataacaat aagaaatatg gtacatcatt
     1141 attttggata cttatatcac tgccattcat atttggtaga ttgattccag ccaatataat
     1201 aggaataata ttaatattat ctagtttatt aacactttct aaacaagttg tatttgcaaa
     1261 gtatgaagaa ccagatgaaa attttggaaa agaacaagca gacaaactta aaaataaaat
     1321 atttatacca tcattaatat tagcatttgc tgcagtagta gtagctatga gcctttctaa
     1381 ttttgataat tcttctcaat ttgcaatagg tgtgggttca ataattgcat taatatcagc
     1441 ccttataata actaaagcaa aacctgctac atcagttcaa gatggctcaa gattacttca
     1501 acaaatggga cctgctagta tgttaccaca acttttagtt gcattaggag ctttatttac
     1561 tcaggctgga gtcggcgaag ttatttcaac catgatatct ggtgtagtcc ctgctgatag
     1621 cagattattt ggtgttatag cttatgtatt aggtatggtt atatttacca tgataatggg
     1681 aaatgcattt gccgcatttt cagtcataac ggcaggaatt ggtattcctt ttgtattgtc
     1741 tcaaggtgga aatcctgcta taattggagc attagcatta acagcaggat attgtggaac
     1801 tctacttact ccaatggctg ctaattttaa tatagttcca gcagccttgc tagaatgtaa
     1861 aaatgattat attgttataa aatatcaagc tccagttgct ttggtattaa ttatagccca
     1921 catattagta atgtactttt taggatttta atataattag gaggtaaaat aatgaaaatt
     1981 ttgttaacag gatttgaccc atttggggga gaactaataa acccagctca agaagcagtt
     2041 gaaagggtca ataataatat aaacggtgca gaaatcataa agataactat accaacagtt
     2101 atgacaaaat ctgttgaagc tatagataaa gctattcaag agcacaatcc agatatagta
     2161 atatcagttg gacaagctgg aggtagattt gacattacac ctgagagagt tgctataaat
     2221 atagatgatt ttagaataaa agataatgaa ggtaaccagg ttatagatac tattataaaa
     2281 gaagatggtg aacctgcata tttttcaaaa ttacctgtaa aagccatggt aaaacatatg
     2341 aatgaaaata aaattccagc tagtgtttca aacactgcag gaacattcgt ttgtaatcat
     2401 gtgatgtatg gaatccttta tatgatagac aagaaatatc ctaatataaa gggtggattt
     2461 atacacatcc catatacaac ttcacaggta ataaataaaa aaaatacacc attcatgtct
     2521 ttagaagaaa tagtaaaagg tttagaatta gctatagaag cttgtattat atacaaagaa
     2581 gatgtaaaag aaattggtgg tgaaatttca taataaattt tttagtattt tataaattaa
     2641 aaatactaac tattaattta aatatagact gtgagtagat tattcaaaca attttactca
     2701 cagtcttttt atggcatcta cagatacaaa attaaggtct attcctctct ccccactcac
     2761 acattgcatc taaaattgga atcagagatt ttcccctttc tgataaacta tattctacct
     2821 taggaggtat ttgaggatat tcagtacgaa taatcaagtt atctgcttct aattctttta
     2881 atgataaact caaagtttta taggaaatag tatttatata acgttttaat gcattatatc
     2941 tgacaacttc aaattctaca agacagtaaa gaattatcat tttatattta ccactaatca
     3001 g
//
LOCUS       DAEPLF010000305         2964 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.306, whole genome shotgun sequence.
ACCESSION   DAEPLF010000305 DAEPLF010000000
VERSION     DAEPLF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.306"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(225..491)
                     /locus_tag="KR314_003460"
     CDS             complement(225..491)
                     /locus_tag="KR314_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBH0329812.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTDIEKPFWGEQCPVKSCCED
                     KNLIHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(534..1343)
                     /locus_tag="KR314_003461"
     CDS             complement(534..1343)
                     /locus_tag="KR314_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329813.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKEKL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEV
                     QNNKRPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            1656..1937
                     /locus_tag="KR314_003462"
     CDS             1656..1937
                     /locus_tag="KR314_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329814.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            2201..2455
                     /locus_tag="KR314_003463"
     CDS             2201..2455
                     /locus_tag="KR314_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329815.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            2500..2823
                     /locus_tag="KR314_003464"
     CDS             2500..2823
                     /locus_tag="KR314_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329816.1"
                     /translation="MATEIFTVKITPLEAQKMIKENIDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTEKTVIKATSTGSAIGVLKIDWGAGGNLLKRVKNIL
                     AEYII"
BASE COUNT         1161 a          311 c          396 g         1096 t
ORIGIN      
        1 aaatttaagt ctattttaat tcaattttgt aacatatatt ttaaatagac agagattgag
       61 attttatatt tctaatttta gtaatcagat tttatcaata aattactatt ctattaattt
      121 aattaaataa cctttaaatt taattataaa aactgttgca gaaatttcta caacagttta
      181 aaaaatttac cttacaaaaa cttattttat aacaagagag tatattataa ggctagtaca
      241 gcccattttt tgcactgaga aattcttttg ccatcatcac cttgttcttt gtcatatgca
      301 aattgattaa gcatttcaca tggaaatgta tcacatagac cacaatgtat aaggttttta
      361 tcttcacaac aagattttac aggacattgt tcaccccaaa atggtttctc aatatctgta
      421 caacctttac aattgacttt ttccttgtat ccacattcat aacataaaat tccacatctt
      481 gattctatca ttttaagtca ctccttaaat ataaatttta ttatgattgt atatcaaaat
      541 ttatactttt cattgtaaaa atcggacaaa ttttttatat aggttgttgg attaacacca
      601 caaatagatt taaaatcatt tataaaatga gattggtcgt aataaccaat atcttgtgct
      661 aaagttgcaa atggtctttt attgttttgt acttcttgta atacaaggtt tatacgaagt
      721 aatctaaggt atgatttaac actcatccca agagaattat taaaaattct atttaaatgt
      781 cgttcactat aacaactaat ttgtgaaatg tttttaacag acataataga gttctgtttt
      841 ctagcatttt caagaattgg tatagtcata tctactatat ttgatttcaa taagtatgat
      901 aagaataaca tatctaattg ttctactaat tcctttatag tacttgttct ttcaaaaatg
      961 gaattaacct ctaagctaaa taatgtgtta aaatcttcaa gtgaaatttt taaatctgta
     1021 gattctcttt gagaaagacc tgtcaaataa tatactccac ctgggcgaaa ctcaacgaat
     1081 actctaaaaa gtacattttc tatatcattt tttacaatag tagtttttgt tgtggctccc
     1141 cagaatgcgc tttctatacc ttttttgtca aatttaaata tcatacatcc actagcatca
     1201 ggtattaatg taagcttttc tttaatactt gtatctggaa tagatattgt atagtgtgct
     1261 atatactgtc ttaacaattt atgtggcaaa atatatgtat actgtttgca ggtatttaaa
     1321 atgttattga ttattttatt cataagtata tcctcctgat atataaaaaa taagtatact
     1381 aatttatttt ttatataata aaccagtttt gttttaattg aaatattcta ataagatatt
     1441 ttgttaaatt tagatacaat aattgtaaag taaaatctca aaatatacaa ttattataaa
     1501 tcatagatat ttattaaata aatatattaa agaagaagga atataaaaca agattagaaa
     1561 ataatagtgt tactttcata ataaatacat ataaagatat ttttaagaat aaaatttatt
     1621 attaaataaa aaataaagga gagtgattta atcgtatggc atcaaaacaa acaccaagaa
     1681 acaatttaga aaagttttta agaataagag tcttatcaca tacaggcgat aaaatacata
     1741 taaagttacc tgtaaatttt gcaaaaagaa tgattgagaa taatgctttg gatttattta
     1801 atggaaaaga tgatgtagta gatggtaaaa aggttactga aatattagtc aaagcatttg
     1861 actatgattt aactggagaa gtagttcact tggagagaaa aaatggagat attattaaag
     1921 tttcaattgt acaatagaat tattttatat gaattttatt tttgaatagt tataaataat
     1981 tagaaatttg taaaaaagta taatatatta ttagtaatgg tgcagaatac acatttataa
     2041 tttatagatg tgtatttttt gtttaaaaaa tttaaatata taaaactata aaggttacaa
     2101 ttttggaaat agatgtaaat atatgttcta ttgtataata agagtaatat aaaatctagt
     2161 aaaaataatc aaataaattt aacttatgag agggataact atggaaaata aggattttaa
     2221 attaacaata tgtatatttg cttttgtaat tggagtagta gtattattct gctgtatagg
     2281 tttgggtgaa tttagtgcag agcatattat tcaacaaaat ggtggaatca tggatactga
     2341 ccaatataat atttatctag accaatctat ttctcaatat agaaattttg gttctatatt
     2401 agctctgtta ggaggaatag ggatattgtt tgataaatat atcagaaaat actagagatt
     2461 atgttttgtc taaaataaat tcttatgagg agataaaaaa tggcaacaga aatatttact
     2521 gtaaaaatta cacccttaga agcacaaaaa atgataaaag aaaatattga tgcagattta
     2581 gtttttagtg attcttataa tttaggagat gataaattta tactaattac aacttttgag
     2641 aagtattacg caagaaataa tagtgatgca ggattaatcg tagtttatga aaataccaca
     2701 gaaaaaacag ttattaaagc tacttcaaca ggcagtgcaa ttggagtttt aaaaattgat
     2761 tggggtgcag gtggaaattt acttaaaaga gtaaaaaaca tacttgctga atatattatt
     2821 taattatttt atctaactga taatatgtca agaaaatctc gtaaaagagg ttaagaatat
     2881 ttttataata tatagataaa taaggatttg aaggtaaagt ttttaatgta taaagaaaaa
     2941 taatataata aaacgaattg gctt
//
LOCUS       DAEPLF010000306         2963 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.307, whole genome shotgun sequence.
ACCESSION   DAEPLF010000306 DAEPLF010000000
VERSION     DAEPLF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.307"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(51..995)
                     /locus_tag="KR314_003465"
     CDS             complement(51..995)
                     /locus_tag="KR314_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBH0329817.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKVCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            1311..2741
                     /locus_tag="KR314_003466"
     CDS             1311..2741
                     /locus_tag="KR314_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0329818.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNADLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
BASE COUNT         1186 a          434 c          360 g          983 t
ORIGIN      
        1 ttgttggatt ctaaaatgta taccaataaa tcctttttaa gtatataatt ctaaatactt
       61 tcagtttttt caatctgctt taacttgttg gatttataaa atattcttat ttcatcataa
      121 gaaagattaa gtgctactac tgcagatatt aacatcaaca tcactccaaa taacttccct
      181 aaggaaaaac tttctcctaa tatagtgcaa ccaattatta ctgaaccaac taactctact
      241 gatgccacaa cacctgcaac tgacaactct acacctttag atatacctgc tgcataaaaa
      301 atatatgcaa gtgcagctgg aacaatgcct aacattagca tacaagataa aacatctaaa
      361 tccttagcat aatttaagat ttcccaaggt ctcgaagaag gaatcataaa tatagctcca
      421 aataagaaac tgtatactaa tattgttgaa cttgaaaatt cctttaaagc atttttacta
      481 atagttggca taagtgcata tgctattgct gataataccc caagtaaaag tccaaaagcg
      541 tttaaacctt gaaaatccaa acgtccacca gtcactgcca taattgcacc aataaaacaa
      601 agaataagtg aaaataattt gcttctagta atattttctt tataacatac ttttgaaaat
      661 attgctaaaa acagtggtga tgtatatagt aaaactgctg caattgatac acctgcaatg
      721 tcaattgctt taaaataaca taaattaaac attgcctgac atataattcc tattattact
      781 gaatatataa ttcctttttt gcttatcttt aagatttgtg gtgtccttat agatgaatat
      841 acgcctaaaa ctataaatcc aaaaaacaat cttgtaaatg ctacttgttc tgatgtcagt
      901 ccatttccca ttaaaaactt tccaaataat ccaagtgtag cccataataa ccctgctatt
      961 gctataaata catacccttt acttttttct cccattttaa attcccttct atttttattt
     1021 agtcaattta cactcttctt tttaagcaaa aattatgcca tcttaaacca atatatatcg
     1081 actctaaaac cctagtcttt ccaataatac taataagttt tcaattttct tatatttatt
     1141 tttcgcttac tttaagttga tttagactta ttttaattgt aaaatgagtt atatataact
     1201 tatttaaagt taaaatagac tgaaacttat ataaatatca aattttaagt atattaatca
     1261 attaaaccta ttttaatata tcattaaaaa tttaggagga aaatataaaa atgaatttag
     1321 atttggaatt ttatcaaaaa atattagaag cttcccatga tgaaatatgt gtttctgatg
     1381 ataaaggaat aataatatat tgtaataaag catttgaaga aaattatggt ctaaaaaaag
     1441 aagatatttt aggaaaaaat gtttcttttt tagaagactc tggatattca acaaaaagcc
     1501 ccataccaat tgtattaaaa acaaagacta aattttcact ggaacaagat actcaaacag
     1561 ggaaaaagct tatcataact gctaccccta tatttgatga aaatggaaac ttagaattta
     1621 ctgttgaaaa ttgtagagat ataactgaac ttaataatat aaaaaataaa cttgaagaca
     1681 caaaaaagca agtaaaaaaa tataaatctg aggtagaaac actatacaga actgcactta
     1741 gaattgaaga tactgtaatt atggacggaa tagtgatgag accaatcata aacacagtaa
     1801 atcatgtttc taaaacggat gtaagtgtac ttttattggg tgaatctgga acaggaaaaa
     1861 gctcacttgc tagatatata caccacaaca gcaatagatc taatggccca tttataacaa
     1921 taaattgtgc aactatatct ccacaacttc ttgaatctga actttttgga tatacttcag
     1981 gtgcttttac aggtgcaagt actaaaggta aagttgggct cgtagaatta gctaatggag
     2041 gaactctttt tttagatgaa ataggagata taccacaaaa tctacaggca aaatttttgc
     2101 aattaataca ggatagaact tttactccag ttgggagctt aaaaaataaa aaggttgata
     2161 taagaattat atctgctaca aatgctgatt tagtttcaaa agtaaaagaa aaaaagttta
     2221 gagaagattt atactataga ttaaatgtta tagaaataaa attgcctcct cttagagaaa
     2281 gaagggataa tctagtggaa attataaaat attattttaa taggtactca agtgacttca
     2341 acctaaataa aactatttct aaagaagcta tggatgctat tgcaaattat agattcccag
     2401 gcaatataag agaattacaa aatataattc aaaaaatctt gcttacttgt acagataatc
     2461 atataacaat agatgactta ccaaacattc ttacaaaaaa tatacatatt accaataatg
     2521 gaaataaaac acatatatcc caaattaata aggttattat atctgactca aaatctacta
     2581 attataaaaa taaaaatttt gacaccttaa ttaaagagta tgaaaaaaat ataattttgg
     2641 atgcttatga aaaatttggt agttcttata aagtagccaa acaccttgaa atctcacagt
     2701 ctaaagccaa taggttaatt agaaagtata ctaatactta attttaaatt tgataaataa
     2761 aaacttaaca ctatttacat attgtaaatt tggccattta ctgttattat gaatatggtt
     2821 gaaattattt aaataaatac tgttgactta gtttcatata tttaccatta aacttttatc
     2881 ataaatatca tatcataata atatatttaa taagtctaat aaactatacg aggtgtaaat
     2941 atgttaatga atacccattt tat
//
LOCUS       DAEPLF010000307         2952 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.308, whole genome shotgun sequence.
ACCESSION   DAEPLF010000307 DAEPLF010000000
VERSION     DAEPLF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.308"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            240..2894
                     /gene="adhE"
                     /locus_tag="KR314_003467"
                     /gene_synonym="adhC"
     CDS             240..2894
                     /gene="adhE"
                     /locus_tag="KR314_003467"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBH0329819.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYTQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDESADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDNAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRSPGKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEEPKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT         1125 a          369 c          561 g          897 t
ORIGIN      
        1 gttaaaaaaa taacgttatc cttaagtttt tagatgattt tattagtgat ttgcgtaaaa
       61 aataaagtga tatctagtta aattaagtat ttgtaaagct agatattatt agtagaaatg
      121 atgttatatt agataaaaaa taaaatgtta aaaaaataac ttgaaaatag aaaaagtaaa
      181 tgctataatg ttaataaatt aacataataa ataaaaaact atctcaggag gttgttaaaa
      241 tggctcataa agaacaagta ataatagaaa ataaaaaaga aaatgaagtt atgtcagttg
      301 gtttagatac tgtaaataac gttgaaagct tagttaaaaa actagcaaaa atcagagaag
      361 ctcaaaaaat atttgctaca tatactcaag agcaggtaga taaaattttt ttagcagcat
      421 ctttggcagc taataagcaa agagttcctc tagctataat ggcacaaaag gaaactggta
      481 tgggaattgc tgaagataag gttataaaga atcattatgc ctcagagtat atatataatg
      541 catataaaga aactaaaact tgtggtgtta tagagaaaga cgaagcattt ggtatgacta
      601 aaattgctga gccaataggt gttattgcag cagttgtacc tacaactaat ccaacttcta
      661 ctgctatctt taaagcactt ttagctttaa aaactagaaa tggaataatt ttctctccgc
      721 atccaagagc aaaaaattct actatagagg ctgcaaaagt tgttcttgag gctgctgttt
      781 tagctggagc tcctgaaggc ataataggat ggatagatga gccatcttta gaattaacta
      841 caactgttat gaaagaagtg gatttgaccc ttgctacagg aggtcctgga atggttaaat
      901 cagcatattc atctggaaaa ccagctattg gagttggtgc aggtaatact ccagcaataa
      961 ttgatgagag tgcagatata aaaactgctg taaactcaat tcttgtatct aagacttttg
     1021 acaatggtat gatatgtgct tctgaacaat cggttatagt attagaaaat atatacaatg
     1081 aagtcaaaaa agaatttaaa gagcgtggtg cttatttatt agataaagat gaaacagaaa
     1141 aaataagaaa tataatttta gtaaatggag gattaaattc taaaatagtt gggcaaacag
     1201 cttgtactat agcaaaactt gcaggctttg aagtcccagt agatactaag gttttaatag
     1261 gtgaagtaga atcagttgaa atagaagaag cttttgctca tgagaaactt tcaccagtac
     1321 ttgcaatgta caaggccagt gattttgata atgcagttag aaaagcagaa aaattagtag
     1381 aagatggtgg atttgggcat acatcttcat tatatattga tgatgtaaat caaagagaaa
     1441 aacttgataa gtttacttca gccatgaaaa cttgtagaat tttaattaat actccttctt
     1501 ctcaaggtgg tataggggac ttatataatt ttaaactagc tccttctctt actttaggat
     1561 gtgggtcttg gggtggaaat tcagtatctg aaaatgtagg agttaagcat ttacttaata
     1621 ttaaaacagt agctgagagg agagaaaata tgctttggtt tagatcacca ggaaaagttt
     1681 atttcaaaaa aggttgttta ggggtagcat taaaagaact taaagatgta atgaataaga
     1741 agagagcttt tattgtaaca gatacattcc tttacaataa tggatatact aaagcagtta
     1801 ctgatttatt agatgaaatg aatattaagc acactacatt ctttgaagta gaaccagacc
     1861 caactttgga atgtgctaag ataggcgcta aagctatgag agaatttaat ccagatgtaa
     1921 taatatctat aggtggagga agtgcaatgg atgcaggaaa gataatgtgg acattgtatg
     1981 agcatccaga tgtagatttc caagacctag ctatgagatt tatggatata agaaaaaggg
     2041 tatatacatt ccctaaaatg ggtgaaaaag ctaattttgt agctatacca acatcagcag
     2101 gtactggttc agaagtaaca ccatttgctg ttataactga ccaagataca ggggtaaaat
     2161 atccattagc agattatgaa ctgatgccaa atatggctat agttgattct gatatgatga
     2221 tgaatatgcc aaaaagctta acatctgctt ctggtataga tgcattaact catgcattag
     2281 aagcatatgt atctatgctt gcaactgatt atacaaatgg attagcttta caagccatta
     2341 agagtatatt tgaatatctt ccacgtgcat atgataatgg tgcaaaagac ccagaagcta
     2401 gagaaaaaat ggcaaatgct tctacaatgg caggtatggc ttttgcaaat gcatttttgg
     2461 gtgtttgtca ctctatggct cataaattag gtgctttcca ccatgtacca catggtgttg
     2521 caaatgctct tttgataaca gaagttatga aatttaattc ttcagatgca ccaaagaaaa
     2581 tgggagcatt ctctcaatat aaatatccag aagcattaaa aagatatgct gaaatagctt
     2641 catttttagg attaaaggga aattctgatg aagagaaatt ccaaagttta ttggtagcta
     2701 ttgaagattt aaaacttaaa gttggtattc ctaaatctat aaaagagttt ggagttgaag
     2761 aacctaagtt tatggattca atagatgaaa tggtgattca agcatttgat gaccaatgta
     2821 caggagcaaa tccaagatat ccacttatga atgaaataaa agatatgtat ttaaattcat
     2881 attatggaag ataaatttac tataaaatag ataaaataaa aactcttata cataaaacaa
     2941 agttttattg ta
//
LOCUS       DAEPLF010000308         2929 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.309, whole genome shotgun sequence.
ACCESSION   DAEPLF010000308 DAEPLF010000000
VERSION     DAEPLF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.309"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            94..1239
                     /locus_tag="KR314_003468"
     CDS             94..1239
                     /locus_tag="KR314_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0329820.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     SRGNRSGNTISIITKKELDIIKDLQKEFSIVITPKTLQNGELIDIIK"
     gene            complement(1353..2729)
                     /locus_tag="KR314_003469"
     CDS             complement(1353..2729)
                     /locus_tag="KR314_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBH0329821.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCLGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFM
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     regulatory      complement(2823..2925)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         1186 a          475 c          381 g          887 t
ORIGIN      
        1 cacataatta taaaagaata tatagttcga tttgaataaa caatgtataa taaaaaatag
       61 ttgactataa ttattaaata taaggagcaa aatatgaata catttgaaca attaaaaata
      121 agttccactc taatagatgg acttaaaaaa caagacataa cttctccaac agaagttcag
      181 tcacttgtta tagggaatat aattcaaaat aaggatttat tgattaattc acagactgga
      241 acaggaaaaa cactagcgta ccttttacct atatttgaaa aaattgatac atcaaaaaga
      301 gaaacacaag ctcttatact tgcacctacg catgaattag taatgcagat tacaaaccag
      361 gttgaattat tggctaaaaa tgcagaactc tccgttactt ctttagcttt aattggggag
      421 gtcaatatac aaaaacaaat aaaaaatata aaagcagtaa aaccacacat agtaattggc
      481 tcttgtggaa gagttttaga cttgataaaa caaaagaagc taaaatcaca taatataaaa
      541 actatagtct tagatgaagt ggacaatcta ttgaatggaa aaaatataac ttgtatagaa
      601 gacataataa gaacaacgtt aagagataga caaattatag gatgctctgc aagtcttact
      661 gatagcacta taaaaatttg tgataaactt atgaaggagt ttgaaataat aaaaacaaag
      721 gaaaaatcac aaataaatcc taacatcaat cactcatatt tattaggtga gataagggat
      781 aaatttactt ttcttagaaa ggcattagca gctacaaatc caaaaaaagc tattgtattt
      841 gtaaataatg aaaaaaatat agaagtccta gtatcaaaac taaattatca taattacaaa
      901 gcaataggaa tttttggaaa tatggaaaaa gaagatagaa agaatgcaat aaataaattt
      961 aaacttggta aagcaaagat tttaattaca actgatttat ctgctcgtgg actagatatt
     1021 gtggatgtaa gtcatgtttt taacttggat tttccaaaaa gtaaaaatga atacctgcat
     1081 agatgtggtc gaacttctag aggaaatcga agtggtaaca caatctcaat aataacaaaa
     1141 aaagaattag atattataaa agatttgcaa aaagagttca gtatagtaat tacacctaaa
     1201 actttacaaa atggagaact aatagacatt attaaataat acaataaata cgaaaataca
     1261 acaaatacaa aagagctgta gtttatcatc acaattgata ataaattata gccctttatt
     1321 ttatattaaa ttaaaatttt aaataatacc tattataata gccaaagttt tattataaat
     1381 aatattgcca gaacaatcat tactacagaa acatgttttt tgtcttctgc ctttttagct
     1441 ccaaatatat tataaaatat atttacgaaa acataagtta atatacctaa agttaaacca
     1501 tcacctatac tatatgttaa tgccatacaa gcaatagtaa caaaagctgg aacaccttca
     1561 gttatattat caaactctat atccttaact gtaccaagca ttaaatatcc aacatatatt
     1621 aaagctggtg ctgtagcaca tgatggaatt gctataaata caggagagaa aaacattgca
     1681 gctaagaata aaattccaac tgttattgct gtccatccag ttcttccacc tgcgataact
     1741 cctgttgaac tctcaacata agttgtaact gtagatactc caagtcctgc accaactgta
     1801 gttgcaaccg catctgtaag taaggctcta cctacattag gaacattccc tttttcatct
     1861 aacatgtttg ctttagaaca aacaccaact aatgtaccta ctgtatcaaa aaaatcaaca
     1921 aataaaaatg tacaaactac tactaaaaaa ccacctatat ttgttggatg tagtacatat
     1981 cctaaatcta ttttacctgc tattggtgct aaactttcga atttaaataa tccaccaggt
     2041 aaatatatac ctaatttttg tgcatactct ggattcataa aagcaaatgc ccaagcaagt
     2101 aatgaactca ctactattcc aaaaagtata gaacctttaa ctcttttttt atctaataca
     2161 gcaataatta ttaatcctaa acaagttatt attactgctg gtgtaaatct acccattttg
     2221 ataagagtgg cttggtctcc aataacaatt ccactgttaa ctagacctat aagtgcaata
     2281 aatattccta taccacctgt aacagctaat ttcatatttt ttggtatcgc atttacaaca
     2341 gcctcacgaa ctccacttac tgataatact ataaaaataa taccttctac aaatactgct
     2401 gtaagagcta cttcccaagg aacacccatt tgtaaaacta ctgtatatgc aaaaaatgca
     2461 ttaagaccca tacctgatgc taaagcaaaa ggaaggttag caactacacc cattaaaata
     2521 catccaaatg ctgctgctaa acaagtacct gtaacaagtg cacctgcagg catgcctgtc
     2581 tcagataaaa tatttggatt tacagctatt atataagcca tagttaaaaa agtagttact
     2641 cctgcaatta tttccttctt catgtcgaca tttttattag tcaaaattgg aaaaagtttc
     2701 tgcatagtac tactgtttgt tgtttgcata acaacacctc ctattaaatt ttacaaaaaa
     2761 acgacacagt agttgataag ctgagccgta tccttcataa ttcagttaat taaatattta
     2821 attaactaaa taatagtata catagtcaga atatttacgg tattccggta gaaacttatg
     2881 acccatattg tcaaaattat atgttataca gtacaaaaca tgaattttt
//
LOCUS       DAEPLF010000309         2863 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.310, whole genome shotgun sequence.
ACCESSION   DAEPLF010000309 DAEPLF010000000
VERSION     DAEPLF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.310"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            117..1664
                     /locus_tag="KR314_003470"
     CDS             117..1664
                     /locus_tag="KR314_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0329822.1"
                     /translation="MNKNSMTKIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIMILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIINLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            1667..2065
                     /locus_tag="KR314_003471"
     CDS             1667..2065
                     /locus_tag="KR314_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBH0329823.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            2105..>2863
                     /locus_tag="KR314_003472"
     CDS             2105..>2863
                     /locus_tag="KR314_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBH0329824.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGVGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIF"
BASE COUNT         1168 a          247 c          473 g          975 t
ORIGIN      
        1 gtaatgtagt tatttatatg tgattttaac ttgcattgat ttgctatcaa ttgtgcttta
       61 taattagtat atataaagtt taactgtaag ataatttaaa aaatagggga atttatatga
      121 ataaaaatag catgacaaaa atttacagtt taaataaaat aatttttatt ataattggaa
      181 ttttaggaat agttttactt acaagacata ttgaggtaga tattaaagtt ttaattttaa
      241 ttttattaac tttgtttagt ataaatgtat ttatttcata tcttaaaatt aagttgtatg
      301 aggataaaat taatttagga ttgataaata attcagataa gtttataaat attactaaag
      361 atgagaactt cctaaaacat gttcaaaatt atattatcag taatgaaaaa gaaaactgtg
      421 taatggcttg ttttgattta tgtagactca aacttataaa tgatatatat ggttatgagc
      481 taggggatga agtgttaaat aatattttaa gtaacttaaa aaattatttt ggaaaagaag
      541 ctatttatgg aaagttaaaa agtgatgttt ttgtattaat aataaagctt gaaaataggg
      601 aacagaagat tccttattta gtaaatctta tcaagaataa aattatgata ttaagtcaga
      661 ttgacagttt taaaatggat ataaatattg aggcttctat tggaatttat aaatttaaca
      721 ataatgaaat tgatataaaa aaagctatag ataatgctga tatggcaagg cttaaatcaa
      781 aaggactaaa gcacatagag tatgtagaat ttgataatgc tatggaagaa gaaatacaat
      841 caattatgaa aatagagaga gatttattct tggctattaa aaataaagag tttgtaattc
      901 actatcaacc taaagttgat tcaagtacag gtaacattat aggttcagag gcattgataa
      961 gatggttgca cccatcatta ggcatggttg gaccaaataa gtttatacct atagctgaaa
     1021 aaaatggtct gataaacaat attggtagat ggctaattca agaagttttt attaccatta
     1081 acaagtggat ttcagaaggt ataaatgtct taccagtctc aataaatctt tctagagtag
     1141 aactatataa aaatgattta gttgattttt ttaaactgat gtttaataca tataatattc
     1201 caaaagaact tatagaatta gaaattacag aaacgacagc gctaagagat gtaaaattta
     1261 taagtgaaag gctttatgag attaaatctt taggtatgaa tatatcatta gatgattttg
     1321 gagtaggtaa ttcaaatttt atcaatctta aggggatacc actagatatt ataaaaattg
     1381 ataggtcact tatattggat atagttacaa atacaaaaac aaagtttatg gtgaaagcaa
     1441 ttattaacct ttcacatgat ttaaacgtaa ctgttatatg tgaaggggta gaggatatgc
     1501 atcaggtcaa agtcttaagt gaactgggat gtaatttcat acaaggttat gtatttttta
     1561 aacctttgga tgagatgaat tataaaaaac ttcttactga cggaagttat ataaatctga
     1621 aagatactct tttagataaa tgcatgacag ttgaggagga gtaaggatga tttcagaaga
     1681 tgtagattat tcaataagaa tcataagagc tatatttaat aatggagaag ataaaccaat
     1741 agatgctaaa atgatattaa aaagtgaaga tataccaata ctaacactat acagactgct
     1801 tagaaagctt tgtgcaaaca atattcttac ttttaacaaa gaagatggca agacatacat
     1861 gttaaatgtg tcattggata caataagtct aagaactatt gtagaagtaa ttgatggaaa
     1921 aatatatata aataaatgta cgagatatgt atctaattgc agtaattgct taaaaggttg
     1981 taaagtaaat cgtgaattgc tagaaataga aaaagaaatt tataaacttt tatcgagaaa
     2041 atctataaga caaatactag aataaaaaat tttcagctat gataataatt ggaggttttc
     2101 ggttatggat gatatatctc aagataattt tctactgtct aaagagtatg agaatagctt
     2161 agatgtagat actaaaaaag caagtggaat atattatact cctaaaataa ttgtagatta
     2221 tattgtaaaa aaaactttaa aaaatcacga tataattaaa aatccatatc cacgaatact
     2281 tgatatttcc tgtgggtgtg gaaattttct tttagaagta tatgatattt tatatgactt
     2341 atttgaggaa aatatatatg aattgaaaaa gaagtatgat gaaaattatt ggactgttga
     2401 taacatacac cgtcatatat taaactattg tatatatggt gctgatattg atgaaaaagc
     2461 tataagtata ttaaaagata gcttaacaaa taaaaaggta gtaaatgatt tagatgaatc
     2521 tgatataaag attaatctat tttgttgtga tagtcttaaa aagaaatggc gatataagtt
     2581 tgattacata gtgggtaatc caccctatat tggtcataaa aaacttgaaa agaaatataa
     2641 aaaatttctc ctagaaaaat atagtgaagt ttataaggat aaagctgact tatatttttg
     2701 tttttataaa aaaattatag atattttaaa gcaaggaggg gttggcagtg ttataactcc
     2761 aagatatttc ttagaaagtc tatcaggtaa agatttaaga gaatatatta aatcaaatgt
     2821 caatgttcaa gaaatagtgg attttttagg tgcaaatata ttt
//
LOCUS       DAEPLF010000310         2860 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.311, whole genome shotgun sequence.
ACCESSION   DAEPLF010000310 DAEPLF010000000
VERSION     DAEPLF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.311"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(66..1106)
                     /locus_tag="KR314_003473"
     CDS             complement(66..1106)
                     /locus_tag="KR314_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBH0329825.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
     gene            1576..2736
                     /locus_tag="KR314_003474"
     CDS             1576..2736
                     /locus_tag="KR314_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBH0329826.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALVVTQILHMLA"
BASE COUNT          980 a          367 c          434 g         1079 t
ORIGIN      
        1 gtataaaaat aaaaattaaa gttaattatt tgttaagaat tgactttggt aaaatatata
       61 attaattatt ctttaagcat ataatttata agtaatttta gtgtattttc aatagcttct
      121 atatgtgttc tttcataatg gtgactattg tttgttccag gaccaacaca tgctatttga
      181 acatcttctc cccaacgaat agcttctgaa gcatctgaac catagaaagt aaatacatcg
      241 acagcgtagt ctaaattatt gtcttctgca acatcgacaa gcttgtttct taaattaaaa
      301 tcatataatg ataaattatc ttttgcaact atagttacac catgttcact agatgtttga
      361 cttggtccag taggtgctat atctattgct acaaattctt ttgttttttc aggtaaacat
      421 ttagaaacac catgtccaat ttcttcataa ttacttatat aaaagttagt agtatgctta
      481 ggcgttaaat tgttttcttt aaaatatcta catatttcaa gaaccattgc tacagctgat
      541 ttattatcta tatatctaga ttttataaaa ccagattctg tagcctcaaa tctatggtct
      601 aggcatacaa aatccccaac acttattcct aaatctaata catcttgctt tgtaaatact
      661 ttttcatcaa gtctaataat cattgtttct tcatttcttt cagtggcagc ttttgtttga
      721 ccatatatat gagtagaagc atacttaaat acaacacttc ctctgatttt tttacctttt
      781 ctagttataa cagtgcagtt ttctccctct atagctgccc aacatccacc accaacttta
      841 tggtatttaa gagtaccatc agatgatatt tcttttacta tagctccaag tgtatccata
      901 tgtgcagata ttgttatttg atttgaatca tcttcaccag gaagagtagc tataagtgca
      961 cctttttttg taagaatagt gtcaagacct aaattttcaa aatctttttt gatttctaaa
     1021 actgcatttt cagtatatcc agctgggctt ggaatgtcca aatatttttt cattaaattt
     1081 atagtattag ttaaatttaa attcatattg tttcctccta aataaatata tttatacaaa
     1141 ttataacata aatacaaatg ttctgaaaac taaaattaaa agaatagaat ctaaacatat
     1201 ttaaaaaaaa agtatataaa ataaatttga tttatattat atagatttaa agtaaaaata
     1261 gttttattta ataattaata aaaataaata caaaacaaaa taaataaata ataaaatatt
     1321 tagtttaatt atctttacaa ataaaaattt tagtaataaa ataattgttg aaaaagattt
     1381 taaattcaga ttattcattt ctaaagtaat taatgtgcaa atctttatta atatatgtca
     1441 atagttaggt atatatgtat ttaattagca tgatatagat aaaagtattt attttaattg
     1501 tctattatct gaaaaagtct ttacttagaa atcataaaaa tcaaattaat taggaaaaga
     1561 aagggtgtgt ataatgtgat tgagactgga tttgtatatt gtagtttctt ggttgtattc
     1621 gtaggtgtaa ttttttttat ggttgataaa ttgaaatgga aggtatttga tgttattaat
     1681 ccaatgcttt taatatactt aggttcagct atacttggat catttggttt atttgcacag
     1741 aatgaagatg ttggaacgta tcaaaacata ataacagtga acttccttcc attaatgtta
     1801 gtgttcttgt taattcaatg tgatttaaga aaaatattaa agatggggcc taaaatgtta
     1861 ttatcatttt tctgtgctac atttacattt atgcttggat ttgttatagc attcataatt
     1921 tttaaaaatt caatgggtgc agattcatgg aaaattcttg gggcagcatc tggttcatgg
     1981 ataggtggct catcaaatat gattgcagca gctaatgctc taggagttgc agaagaagga
     2041 cttagttatg caatacttat gggttctata ggatatacag tatggatgtc tgtttgtcta
     2101 atgtctgtga aatttggacc taaatttaac aaatggacaa agtgcgatac aagttttata
     2161 gatgaggtta catcaaaact tgaaaaagaa aataaagata atacggcacc tacgtttgta
     2221 gaacttatga ttttactttc tgttgggttt ggagttgcgt cttttgcaca aattgtatcc
     2281 aagatacttc caacttcagg tatacttaat gaaactatgt gggtagtact tgttgcatct
     2341 acagtatcaa tattattggg tatgacaaaa atatcaaagc ttaaagggag tacactggta
     2401 ggaaatgtgt ttatgtattt actccttgca gctacaggta gtaaagcaaa tttctttgga
     2461 ttgtcagaag cacctgttta cataatgttt ggattggtag tagtccttgt acatgctatt
     2521 tcatttatag ggcttgctaa actatttaaa cttgatttat ttacttgtga agtaggaagt
     2581 atagctaata taggaggagt aactacagct ccaatgattg ctggggttca caatcctgta
     2641 ttaataccag taggtgtact tatggggctt ctaggaaata ttatagggac ttattgtgct
     2701 cttgtggtaa ctcaaatatt acatatgtta gcttaataaa aatatggtat aaaattaata
     2761 atttaaataa gaaaagtcat ttcaatttaa gcagaagctt ttactgaaat ggtttttttt
     2821 gtttgacaag ataatctatt tcaagaagat gttttgacat
//
LOCUS       DAEPLF010000311         2836 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.312, whole genome shotgun sequence.
ACCESSION   DAEPLF010000311 DAEPLF010000000
VERSION     DAEPLF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.312"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..2118
                     /locus_tag="KR314_003475"
     CDS             <1..2118
                     /locus_tag="KR314_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBH0329827.1"
                     /translation="IYLLLCNHTYYVYVYSLNNRVVYPYYELDSINKKLSKKSEQLGK
                     INLAIEKDMIIQRTLKNYIDQRKELLRQALDTIPNVWIVTDYDFNISYTNNKFKDEFS
                     KDMNNYYKILTFIKEDEKVAEKLKKFDNDISIIDKLVELNDKIYLLSLSNNKDESNYL
                     ISLNDITNEIKIDEEIRNINKDYENIILNIPSPILVRSAEGGIKKNKVISINKKYEKS
                     FKCVSSDLVDMTLEQYFETFNIDFFDNRKFKRLNLTQEKKAEIVSYNDTANCIVNFVM
                     SDSEGEEHVEEVRVGDYWSDNKLFKLLTFRDITKEINILRTVNEQKIIYEKLLDVIPE
                     AIFLEDLSTSRVLYTNRAFRELFGISSDVLGVTTQKYRNILVKKYINNLNIGEREKSI
                     HIVNENNHIKEVKMISRTLYFGQKRSRVRIIKDLSVQRESERLKKALIKQRQYDQMKM
                     EFYANISHELKTPLNNIYSSVQLIENLYKKGKIIDFQDILKEHIKITKQNMFRLLRLI
                     DNIINISQVKSDIYKIKAVNFDIIDITERIVTSISSYAKSKGIDLIFDTDEETVMVGL
                     DPESIERIILNILSNAIKFTLEGGEILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFN
                     RFKQIENSGISNEFGSGIGLCLTKSLIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEE
                     VYDNSNYNDNIEKFEIEFFDIYK"
     gene            2227..2835
                     /locus_tag="KR314_003476"
     CDS             2227..2835
                     /locus_tag="KR314_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBH0329828.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
BASE COUNT         1263 a          230 c          463 g          880 t
ORIGIN      
        1 atatatttac tattatgtaa ccatacttat tatgtatatg tatacagtct taataataga
       61 gtagtgtatc catattatga acttgattca atcaataaaa aattaagtaa aaagtcagag
      121 caattaggaa agataaattt agcaatagaa aaggatatga ttatacaaag aacattaaaa
      181 aactacatag accagagaaa agaattactt agacaagctc tggatacaat accaaatgta
      241 tggatagtaa cagactatga ttttaatata agttacacaa ataataaatt taaagatgaa
      301 ttttcaaagg atatgaataa ttattataaa atattaactt ttataaagga agatgaaaaa
      361 gttgctgaaa aattgaagaa atttgataac gatataagta taattgataa actggtagag
      421 ttaaatgata aaatttattt gttaagttta agtaataata aagatgaaag caattactta
      481 ataagtttaa atgatattac aaatgaaatc aaaatagatg aagaaataag aaatataaat
      541 aaagactatg aaaacattat actaaatatt ccatctccta tacttgtaag aagtgctgaa
      601 ggtggcataa agaagaataa ggtaataagt ataaataaaa aatatgaaaa gagttttaaa
      661 tgtgtttcaa gtgatttagt cgatatgaca ttagaacaat attttgaaac ttttaacata
      721 gatttctttg acaataggaa atttaaaaga ttaaatctaa cacaagaaaa gaaagctgaa
      781 atagtaagtt acaatgacac agcaaactgt atagttaatt ttgtgatgtc tgactcagaa
      841 ggtgaagaac atgttgaaga agttagagtt ggagactatt ggtcagataa taaactcttt
      901 aaattgctta catttagaga tataactaaa gagataaata ttcttagaac agtaaatgaa
      961 caaaaaataa tatatgaaaa attacttgat gttataccag aggctatttt tcttgaggat
     1021 ttaagtacat caagagtact atatacaaat agagccttta gagagttgtt cggaatttct
     1081 agtgatgtat taggagttac aacacaaaaa tatagaaata tattagtaaa aaaatacata
     1141 aacaatttaa atataggaga aagagagaag agtatccata tagtaaatga aaataatcac
     1201 attaaagaag taaaaatgat atcaagaaca ttatactttg gacagaaaag aagtagagtg
     1261 agaattatta aagatttaag tgtgcaaaga gagtctgaaa gacttaaaaa agcattaata
     1321 aaacaaagac agtatgacca aatgaagatg gagttttatg ctaatatatc tcatgaactt
     1381 aagacacctt taaataatat atatagttca gttcaactta ttgaaaacct ttataaaaaa
     1441 ggaaaaataa ttgattttca ggatatacta aaagaacata taaaaataac taaacaaaat
     1501 atgtttagat tattgagatt aatagataat ataataaata tttctcaagt taagtcagac
     1561 atatataaga ttaaagcagt taattttgat attattgaca taacagaaag aatagttaca
     1621 tctattagta gttatgctaa gtctaaagga atagatttaa tttttgatac ggatgaagaa
     1681 actgttatgg tagggcttga cccagaatct atagaaagaa taatactaaa tatattatca
     1741 aatgctataa aatttacttt agaaggagga gaaatccttg taggtatata taaaaaagac
     1801 gaaacagtag agataattat aaaagatact ggagtgggca tagacaaaga aaaattaaat
     1861 gatatattta atcgatttaa acaaattgaa aacagtggta tatcaaatga atttggaagt
     1921 ggtataggtt tatgccttac aaaatcattg attgaaattc aaaatgggaa gatttacata
     1981 gatagtaagg taggagaagg aactaatgta aaagttatat tcccaattaa agaggttgtt
     2041 gaagaagtat atgataattc aaattacaat gataatattg aaaagtttga gatagaattt
     2101 tttgatatat ataaataata aatggctaaa tttattgttt atatatatca cattaataag
     2161 agttaatttt atttattact ttagttgtat ttgatgcgtt ttaaataaat tttatgaggt
     2221 gaaattttgg aaaaggattt ttttagaaaa aatggaattg atttagccaa atcaatatta
     2281 ggtaaatatc taataaggaa atatgaaaat aaagtaattg ttaccaaaat catagagaca
     2341 gaagcataca tgggtgtaaa tgataaagga gcacatgtat atggcaataa aaagacagat
     2401 agaactaagc cattatattt agatggtgga catatatatg tttatttaat ttatggtatg
     2461 tataattgct taaatttatc tgcaaatata gaaaatgtgc cagagtgtgt attaataaga
     2521 ggggtagagc ctataacttc tttagatgag atatctatga atagatacaa caaagcttac
     2581 actgagttga gtaaatatca agtaaaaaat attactaacg gaccaggaaa actttgcaaa
     2641 gctttaaaaa tagatagaag tctaaatagt aagagtataa tgggtgaaga attatatata
     2701 agtgattttt attatgatga taaaggaaaa aaagtattta gtaaagatga gttagatata
     2761 aaaactagta aacgtataaa tatagattat gcagaagaag caaaagattt tttatggcgt
     2821 ttttatatag aataag
//
LOCUS       DAEPLF010000312         2786 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.313, whole genome shotgun sequence.
ACCESSION   DAEPLF010000312 DAEPLF010000000
VERSION     DAEPLF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.313"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..721
                     /locus_tag="KR314_003477"
     CDS             <1..721
                     /locus_tag="KR314_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBH0329829.1"
                     /translation="KTVSNEKCPFEIIQIGAIKLDSKFNIIDTFNSYVKPTIYKDIHP
                     FISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCVWGVGDLKELYRNINYHKLP
                     SSSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELDEKMSYHNALNDAYYTAKVF
                     IKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDEFRKILNRELTKDEKKIINL
                     AYNMGKTNQFTLENVKQRKNK"
     gene            complement(813..1565)
                     /gene="xth"
                     /locus_tag="KR314_003478"
     CDS             complement(813..1565)
                     /gene="xth"
                     /locus_tag="KR314_003478"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBH0329830.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(1726..2676)
                     /gene="galT"
                     /locus_tag="KR314_003479"
     CDS             complement(1726..2676)
                     /gene="galT"
                     /locus_tag="KR314_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBH0329831.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
BASE COUNT          966 a          467 c          286 g         1067 t
ORIGIN      
        1 taaaactgta tccaatgaaa agtgtccttt tgaaattata caaataggag caattaaatt
       61 ggattctaaa ttcaatataa tagatacgtt taatagctat gtaaaaccta caatttataa
      121 agacatacat ccctttatta gtcgtatgac taatattaaa aattctgatt ttaatgattc
      181 gcctactttc cctgaggtct acaacaattt tataaaattt atatcatcac aagaccctat
      241 attatgtgtt tggggagttg gagatttaaa ggaattatac aggaatatca actatcacaa
      301 gctaccatca agttccttac ctaaatctta tattaatata caacaacatg catctaaata
      361 tttcaataat ccagcaggaa aaagcattgg cctacaaaat gctatttcta tattggaact
      421 tgatgaaaaa atgtcttatc acaatgcttt aaatgatgca tattatactg ctaaagtttt
      481 tattaagata tacaatccaa gtatagtccc agatatatat ctttatacga gtataaaacc
      541 taaaactata agatattcta ataaaaaaag agttgattat gataagttat ttgatgaatt
      601 taggaaaatt ttaaatcgtg aattaactaa agacgagaaa aaaatcatta acttagctta
      661 taatatgggg aaaacaaatc aatttacatt agaaaatgtt aagcaaagaa aaaacaaata
      721 gagtttattt agataaaaaa taggcatgta gattaaaaaa ctagttaact ttaaatttat
      781 atctacagcc caatttttat cgtaattaat ttttatttta tctctaattc aactggacaa
      841 tggtctgaac ctaatatttc agtgtgtata tccgcactta ctaatctatc ttctaatttt
      901 ttggatgcac aaaaataatc tattctccac cctgcattat tttttcttgc attaaatcta
      961 tatgaccacc aactataaac gccttcttta tcaggattaa aatatctata tgtgtctata
     1021 aacccactgt ctaataattt agaaaacttc tctctttctt ctttagtaaa acctgcattt
     1081 ttcatattat ttttaggatt ttttaaatct atttctttat gtgcaacgtt catatctcca
     1141 cacacaatta caggcttatg attatctagt ttggttaaat aatctataaa gtcgtcttcc
     1201 catctagttc tatattctag tctctttaat tcagattgag aatttggcgt atatactgta
     1261 acaaagtaaa aatcttcaaa ctctagagtt atcactctac cctctttgtc atgttcttct
     1321 atatttattc catacattac ttttaaaggc tctttttttg taaatatagc agtacctgaa
     1381 taaccctttc tctcagcata attccaatat tgaaaatatc ctggtaaatc taactctatt
     1441 tgcccttctt gcaatttagt ttcttgtaaa caaaatatat ctgcatctac ttctttgaaa
     1501 aagtctaaaa atcctttccc aacacaagct cttattccat taacattcca tgatataaat
     1561 ttcatactta tactcctcat ctaaacattg acacactccc catagctaaa gatgggggct
     1621 tttcaacaat tttattgtaa attcttaatt tacttttatt ttaacaatac tatactataa
     1681 tttcatctat aattactatt tacttaatac ctaaaaatat atgttttaat caatttttaa
     1741 cttttttgtt aaatcttcag ggctcattga agaaacatac atatttgttc caagttcaaa
     1801 tcctccaaaa ttatattttc ttgggattat gtgcatatga acattaaaat acgattttgt
     1861 ttcaatcttt gttggatgag tgtgaataca taagttaaat ggcatatatc cattgatatt
     1921 gtataacttc ttaaacaact tttcaaatat tgtagaaagc tcctgtatat tattatcatt
     1981 taactcacca aactctattt tatctttaaa tattattctt acttctccac tatacttggt
     2041 tgcatatggt ataagtacca aaaaagcttc tccattataa acaactcttc tatctaaact
     2101 tatttcattt tctattaaat catcatacaa agagctttta ttgctgtcat aatactcctt
     2161 tgaaactaac agttcattcg taatttcagg tggaataaaa gacatagaca atatttgagc
     2221 atgtggatgc atcaaagatg ctcctgcctt tctcaagaag tttttgaaga tactaacata
     2281 ctctacttta tcatctttcg acaaatctct aaatctattt ctatacataa aaaaaacatc
     2341 ttcaaattct ttttgactca tgttataaaa actacgatta tgtttatcgc tctctatcat
     2401 tacttcatga acaccatata catctcttgc catatcatca attataggaa atttattgta
     2461 gacagatttt acaagccatc cttcttcact ttctatttca aataatgcat cagttgcatg
     2521 agcttcattt cctttgcaaa atgggcattc ttcatcatat tcattagttt cttcatcttc
     2581 atttgggata tctgctgtat ctaaaggtct ttttaaccta tctgtagcaa atataacaac
     2641 atcattagta attggatcta ttcttaattc tttcataatt aacacctctt agttaatagc
     2701 ctttaatata attatatgat atttatcgta tctgttaaat aaatttataa tattttttat
     2761 aattttgcat atttattatt atatat
//
LOCUS       DAEPLF010000313         2784 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.314, whole genome shotgun sequence.
ACCESSION   DAEPLF010000313 DAEPLF010000000
VERSION     DAEPLF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.314"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..1073
                     /locus_tag="KR314_003480"
     CDS             <1..1073
                     /locus_tag="KR314_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBH0329832.1"
                     /translation="IDLYDDNNNEVAKKVNFSIFNPKFNLYMYQYKNLINYLDKSICM
                     GALSSGSTSVPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSV
                     LYEGGSIVISSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGS
                     QLLFEYTARNLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYI
                     RGGKIFVNTEYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIVNIGGFKVSSAKIEN
                     EVKKIPQIEDAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKII
                     FLNSFPYTSSEKIDRLALLKML"
     gene            1149..2024
                     /gene="dpsA"
                     /locus_tag="KR314_003481"
     CDS             1149..2024
                     /gene="dpsA"
                     /locus_tag="KR314_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBH0329833.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEVGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            2027..2617
                     /locus_tag="KR314_003482"
     CDS             2027..2617
                     /locus_tag="KR314_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBH0329834.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
BASE COUNT         1063 a          341 c          457 g          923 t
ORIGIN      
        1 ttattgattt atatgatgat aacaataatg aagtagcaaa aaaagttaat ttttctattt
       61 ttaatcctaa atttaatcta tatatgtatc aatataagaa ccttataaat tatttagata
      121 aaagtatttg tatgggagct ttatcttcag gttctacaag tgttccaaag gtattatata
      181 gaacatatga gagttgggct ggattttttc ctatacaaaa tggtattttt aatatctctg
      241 gggaatcaat cttgtttgtt aatggaacac ttagttttac agggaatcta aattctataa
      301 tgtcagtatt atatgaagga ggtagtattg taataagttc aggtcttaat tgtaaatcat
      361 ggataaggac aataaaacaa tataatgtaa ctaacatata ccttatacct acaaaacttc
      421 aacttttggt taaacactta aaagaaccag tactcaaagt agtaagtatt tttacagggt
      481 ctcaattatt atttgaatat actgcaagaa atctaaaaaa atatatgcca aagtctgaaa
      541 ttattttata ctatggggca agtgaattaa attatattac atatttatgc tatgatgaac
      601 taattgaaaa acctctaagt gtaggcagac catttccagg aatagatata tatattagag
      661 gaggaaaaat ttttgtgaat acagaatatg cagtatataa tgcaaccaaa ccttattctg
      721 tcaatgatat tggatattat gataatgatg gatacttaat atttgaagga agaagtgatg
      781 acattgtaaa tattggagga tttaaagtta gttctgctaa gattgaaaat gaagttaaaa
      841 aaattcctca aattgaagat gcaattgtat tgccgtactc agacactata agaggaaatc
      901 aaattgtatt atttgtaata actatagaca aaattacgaa gaaagatttg ttgattaaaa
      961 tgaaagataa tctaatgaaa aatgaaatcc caaagaaaat tatcttttta aattcatttc
     1021 catatacctc ttctgaaaag atagatagat tagcattatt aaaaatgtta taagtataat
     1081 atttagtaat tttagtgaac caatgtattg ataaaaacat attgtatata gtaaaggagg
     1141 cttacaatat gtcaaatcaa tacgatataa ctgttattgg gggagacctg cgacaagtgt
     1201 acatggtaaa aaaactaatt aataaagggt attctgtagt cacttatggg atatcaaatg
     1261 aaattataaa tggaatcgtt ggaaaatcaa cttcactagc tgaagcacta tccaaaagtt
     1321 ccattatttt atgtccaatt ccatttacaa agaatcaagt taatattgaa aatacggatg
     1381 attgtcttga tgctactatt gataacttgc taattaattt atctcctaat caaatattat
     1441 ttggaggtaa cataccaact aaagtttgta aattttgcga gcaacactct attcagattt
     1501 atgattttat gaaaatgaat gatgttgcta ttttaaatgc agttgctaca gctgaagggg
     1561 ctatagcaga agctattaaa agaagtataa tcaatataca tcaaagtaac tgtttagtat
     1621 taggttatgg aagatgtgca caaatacttg caaaaaaatt atacgctttg gatgcaaatg
     1681 tatgcgttgg tgcaagaaat aatgatgcaa gaagtacggc taatgcattt ggatattcca
     1741 gtatttcttt aagtcaactt gatgaaaaac ttttacaatt tgattatatt tttaacacca
     1801 taccagagtg tattttaacg aaagaaagat tagaaaaagt atcacaagaa gtaactatta
     1861 tagatatagc ttcttctcct ggaggagtag attattctgt tgttaaagaa gtagggatta
     1921 atgcgactct ttgccttggg cttcctggta aatattcacc aaagacatct ggtgagattc
     1981 ttgttaacgc tatagaaaat attataaatg aaagaaggga ttagaaatga gacttgaagg
     2041 acttacaatt ggagtaggat ttactggttc gttttgtact tatgataaaa tatttataga
     2101 gttagagaat ttggttaaag aaggagctaa tgtacacact atattttcag atgtatcaca
     2161 aaatatcgat tgtagatttg gaaattctga agagtttatg aaaaaagcat atgaacttac
     2221 aggaaataaa cctatagtta cgattgaaga ggcagaacca tttggaccta aagggattgc
     2281 tgatattata ataatagctc cttgtactgg aaatactgct gcaaaattag caaatggtat
     2341 tacagattca ccagtattaa tggcagcaaa aggtcatctt cgtaatgata aaccactagt
     2401 aatatcaata tcaacgaatg atgctctaag ctttaatttc aaaaatattg gtattctcct
     2461 taattctaaa aatatatatt ttgtaccttt tggtcaagat aattgcaaag caaagcctaa
     2521 ttctatgatt gcacatactg agctaataat tccaactata gaacttgctc tagagaacaa
     2581 acagctacag cctgttataa aaagtccaca tcaatagaat tggttcttat aatattatgt
     2641 attagataca atactagcaa agaggctgtc tcaaaataga gagaagcctc tttttcattg
     2701 ttgaaatgat gaaaatatat ttaatttcat acaaaaaaat gcatcatgaa ctaaaaagtc
     2761 tattttgagc taatctccgt aatt
//
LOCUS       DAEPLF010000314         2777 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.315, whole genome shotgun sequence.
ACCESSION   DAEPLF010000314 DAEPLF010000000
VERSION     DAEPLF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.315"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            367..834
                     /locus_tag="KR314_003483"
     CDS             367..834
                     /locus_tag="KR314_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0329835.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDILGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKGIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEIGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
     gene            2006..2719
                     /locus_tag="KR314_003484"
     CDS             2006..2719
                     /locus_tag="KR314_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0329836.1"
                     /translation="MINIIICEGKKSERVILNDYINTFFYNYNFEYNIKEFENGGDLL
                     KKYPNNVDIIFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRY
                     LEKPLKYSLFKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHT
                     EKGIYTFKNSMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSR
                     YKIKLLREKLNSCLVDVII"
BASE COUNT         1177 a          168 c          378 g         1054 t
ORIGIN      
        1 tatttgaata atctttttaa tcatataaaa tgcatatact tgtaatattt aaaggtgata
       61 ttaatattat tagtgtatat taatattccc ttgaaattaa aaaaacatag atatttcagt
      121 aatctaaaat agtctacaaa aataacagtt ttagcaaaat taataagaat acatatagat
      181 ttaaacctaa aagtatgaac atgtctgttt tttacaagat taaaatttat attatatgat
      241 aaaattaact taactatttc aataagactt ataatttttt tctttatact agaccttatt
      301 gaaaccttta tagtttaatt ctaattatat attataaata aagtatgtgt atattaagga
      361 ggtaatatga agtttttaaa tgttttaata gtagttgaag atattgagaa atcaaaaaag
      421 ttttattatg atattttggg gttaaaggta atctgtgatt ttggagaaaa tgttgtatta
      481 gaaggtaata tatcattaca agaaaaaaag ctttggttag aatttattaa taagagtgac
      541 agtgaagtta agttcaatgg aaatgatgct gagttatatt ttgaggaaga taattttgat
      601 acttttgttg aaagattgag tacaatgaaa ggtatagatt atgttcatct agctatagag
      661 cataggtggg gtcaaagagc aattagattt tatgatttag atgggcatat tattgagatt
      721 ggagaaacta tgtcatctgt gtgtagacgt tttttagata gtggtctttc aatagatgag
      781 gtagctaaaa gaatggatgt aactgtcgaa tatattgaat cagttttaga atagatggat
      841 tatattaaag taaaactata ttttgtatag aagtaagggc tgtaagtaaa cttgtatgga
      901 aacaaattta cttacagttt ttgtttttgt aagatacaga atataatatt tgttataatt
      961 ttatttggat ttttaaaaag tgcatgattt tccttacata ttataataga atttaatatt
     1021 tattggatga tttaaattta gaaaaatata agtattagct tataaaagat aattatactt
     1081 actatcaagc tgtagaatca tatatactat tttatgtaat tatgttaaaa tttaattgaa
     1141 caaattaaaa atatagtgaa aattgtagtt ttagtttatt tatttaataa atatttcaaa
     1201 taaaataatg tttaaataag aaacataaat aataatatta ttattgttct taaagacaaa
     1261 aaatcttagc ttaaaaattt gtatcatata aaatccctac atattatgga agtaatgata
     1321 cattatatga tatacacaat aaagtataaa agtatttaca tgttttttct atatatttta
     1381 taagttttag gtatggaaaa ttactaaata aaaattagat taatgattat gatagtatag
     1441 ataaagttta ttctttttat taatagaaat tgttgtataa aaataataaa agtgaggaga
     1501 gtgatattta aataaaaaaa ttttaataag tattggtaat atagttttta gggatgatta
     1561 ttttatgtaa aaagttgtta tttgtaaaga taaatataga aaatattata agtattgatt
     1621 tagtgataat ctaaaaggtg agaattgtaa taaaaagaga acatatcctt caaatggatg
     1681 taaacttata aataacatta ttacaagaga tttattatac atatactgca actattaatt
     1741 agaatttata ctgtatttgt atttaaataa aaacacaaat aatattattg taaaaattta
     1801 taattattaa atatttaaag tataagatat aataattata agtaatttat gtataaatgt
     1861 ataaaatata gatatattta gattaaataa taattattat tatacaaaaa ttttgtatta
     1921 aaaaatatct ttaaatttga aatataataa atgttagata gaattttata aaatttaaaa
     1981 taaaaaatta tataaggggt gtaatatgat taatattatt atttgcgaag gtaaaaaatc
     2041 agagagggta attctaaatg attatataaa tacctttttt tataattaca attttgaata
     2101 caatatcaaa gagtttgaaa atggaggcga tttattaaaa aagtatccta ataatgttga
     2161 tatcatattt atggatatag acttaaatga tgaaaatgga tttaatgttt caaaagaaat
     2221 caaaaaattt gatgaaagtg taagaattat atttgtatcc tcatcaaata aatacataaa
     2281 gaaaggttat gagataggag tttatagata tttagagaag cccttaaaat actctctatt
     2341 taaagaagca atatcatcat atttaaaaga atgctacaaa aatgaagcta ttttatctat
     2401 aaaatgtaaa tgcaagatat atttcataaa ggtagatgat attttatatt tagaagtttt
     2461 aaaaaatgat ttaactatac atactgaaaa aggaatatat acttttaaga atagtatgaa
     2521 agaaatagaa tcaaatctag aagaacataa tttttttaga tgtcataaaa gctaccttat
     2581 aaatttaatg aaggtaaata tgctagatat agaaaataat attgtattta taaatcaaaa
     2641 ttcaataatt gtaagtcgtt ataagataaa attattgaga gaaaagttaa atagttgttt
     2701 ggtagatgta ataatttgag gtaaattttt gcccttatat tctattctaa tagaatatat
     2761 ctattgtaaa atatgaa
//
LOCUS       DAEPLF010000315         2777 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.316, whole genome shotgun sequence.
ACCESSION   DAEPLF010000315 DAEPLF010000000
VERSION     DAEPLF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.316"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..506)
                     /locus_tag="KR314_003485"
     CDS             complement(<1..506)
                     /locus_tag="KR314_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329837.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVKTLAVFLI
                     IFIVIGIR"
     gene            complement(493..1260)
                     /locus_tag="KR314_003486"
     CDS             complement(493..1260)
                     /locus_tag="KR314_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329838.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALKGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTMDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            1548..2222
                     /locus_tag="KR314_003487"
     CDS             1548..2222
                     /locus_tag="KR314_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0329839.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            2246..>2777
                     /locus_tag="KR314_003488"
     CDS             2246..>2777
                     /locus_tag="KR314_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0329840.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLINEKISDDIDSEI
                     FRIENSVEKILYLSRLE"
BASE COUNT         1051 a          363 c          321 g         1042 t
ORIGIN      
        1 tttcttattc caataactat aaatattatt aaaaataccg ctagtgtctt aactaaggcc
       61 tttatactta agttcatatt tactactatc atttcattca ttaattttac aagcatcata
      121 gtcataagct ttgaaaataa cagtcctaaa aatataccta ttagtattgc aactataccc
      181 atcaaaaaag tttcatataa aaatattttt cctatctgat ttttcctcat tccaagtaaa
      241 ctataagttc ctacttctcg cctacgtcta ttgaaaaaaa atgagtttga ataatatata
      301 aataaaaata caaataccac tattacaatt gaacttgtat taatactcat cctagttttt
      361 tctccaagtg aactcaacgc ttcattatac tctatagatt taaaactaaa aaatacaaat
      421 acactaaata caattgaaat aaagtacatt aaataattaa aaaaactatt ctttacattt
      481 tgcattgcaa ttctaaataa agtcattttt attaactcct ccaattaagg aaactgtatt
      541 tactatttta ttatagaact ctctgttact ttctcccttt acaatttctg tgaatattat
      601 tccatccttt ataaagagta ttctattgca aaaactcgca cttatagcat catgagttac
      661 catcaatatt gttttattgc tgtctttgtt taattttaat agacaattca acaaatccct
      721 agcagattta gaatctaatg ctcctgtagg ctcatctgca agtactaatg atggttttgt
      781 tgctatagct ctacatacag ctcctctttg cttttgtcca ccagaaactt cgccaggata
      841 tttatctaat atagattcta tatttaattc ttttgaaatc tcttttattc tattttctat
      901 tactgatact ctttcatttt ttaaagaaag aggcaacatt atattttctt ttattgtaag
      961 tgtatccaaa agattagaat cttgaaatac aaagcctaga ttatcacgtc taaaatctgc
     1021 caattcattt tgcttcattt taatagtatt ctttccattt atataaatat ctcctgatga
     1081 aggtaaatcc attgtagaaa taatatttaa aagtgttgat ttaccagcac cggaagaccc
     1141 cataatacca acaaattctc ctttataaac cttgaaagtt attcctttta gagcttcatg
     1201 ttttgaacct tttcttccat actccttttt tatattttca acagataata tttcttccat
     1261 agttaataat cacctttcat cacaattagt tatttatcaa ttctcacaaa tatattatat
     1321 atattaaaat ggcttttaaa catttaatta tattaattta aggttacatt ttcgtaagta
     1381 ttaattatat ttcttcaaat ttattgcaat tatgtttaaa atataattta tcaagtaaaa
     1441 tatagttata ttaatataat tattttaggt aataaggtag aaatatttta atttatcaag
     1501 tagaatatag ttatgttaat ataactattt tgaattagga ggtaagaatg tttaaaataa
     1561 tggtagttga agatgacgtc tcattgaaaa atatcatagc aaagtgtctt actaaatggg
     1621 gacatgatgt tcatcaaata gaaaatcttg aaaatataat agaagagttc aaaaactata
     1681 acccagaatt agttttattg gacataaacc ttccatttta tgatggtttt cattggtgta
     1741 atgaaataag aaaaatttca aaagttccta taatttttat ttcttcaaga aattctaata
     1801 tggatgtaat aatgggtgta aatttaggtg cagatgatta tatacaaaaa cctttttctg
     1861 tagatgtctt agtagctaaa gttaatgccc ttttaagaag aacttataat tttgttgata
     1921 ataactcaaa tcagattatt cacaatggtg ttactttaga tttatcaact gcaacaataa
     1981 actatgaaga taatactatt gagctaacaa aaaatgaaat caaaattctt catgagctta
     2041 tgaaatataa ggggcagata gtaagtagga ataaactcat gaaaaaatta tgggataatg
     2101 attggtttgt agatgataat acattaactg tcaatgtaaa tagaatacgc tcaaaattaa
     2161 atgaaattgg tttagaagat tttatagaaa caaaaagagg attgggttat ataatcagtt
     2221 aaaacaattc taagtaggtg aatttatgag ctttaaagat tttctcaagg ataaactagg
     2281 ttttttagta tacaatttta ctttcttaat ttttacagta tcagttatta tattttctcc
     2341 tgtagatgtt tttctgactg atactatttt atatatatta gttgtaaatg ttgtgttttt
     2401 atttttatat ctatttatta gctacatcaa gaagaacaaa tttttagata agataaaaaa
     2461 tgatacattc aaaattaata tgaatgatat agaattagct agatgtaaaa atgaagaaaa
     2521 aatatacttg aacattatta aaaatcatca atatcaatgt aataacataa taaataataa
     2581 tatcgagaaa tttgaagaaa acaaagagat tttaaatatg tgggtacacg atgtaaagat
     2641 acctatagct ataataaaac tcattcttga acaaaatgaa aatttaatta atgaaaaaat
     2701 ttcagatgac atagatagtg aaatatttag aattgaaaac tccgttgaaa aaattttata
     2761 tttatcaaga ctcgaag
//
LOCUS       DAEPLF010000316         2732 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.317, whole genome shotgun sequence.
ACCESSION   DAEPLF010000316 DAEPLF010000000
VERSION     DAEPLF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.317"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            175..1347
                     /locus_tag="KR314_003489"
     CDS             175..1347
                     /locus_tag="KR314_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0329841.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAE
                     RLKLEFEKEGLDCELVDVGENGNTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGALCAFNMETGLVDNSLCIGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSRENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            1361..2008
                     /locus_tag="KR314_003490"
     CDS             1361..2008
                     /locus_tag="KR314_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329842.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            2010..2519
                     /locus_tag="KR314_003491"
     CDS             2010..2519
                     /locus_tag="KR314_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBH0329843.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
BASE COUNT         1021 a          275 c          571 g          865 t
ORIGIN      
        1 acaaaaggat aattgaaaga aaaatcagaa aatataaaaa agactgaaaa atatgttgac
       61 tggcaagaat aattgtgcta ttttgtaatt aaggactagt cttttaagat ttcctaaaat
      121 aaacttagat tttaggaaag aaatacattt tatttgaaaa taaaggagaa gattatgaat
      181 acatatttaa atgaaatttc taagtttgtt gatgagaata gagaagaaat agtttcatta
      241 tggaaagaga ttgttaatat agaaagttat acacattgta aagaaagtgt aaacaagtta
      301 gctgagagat taaagcttga atttgaaaaa gaaggtttgg attgtgaact agtagatgtt
      361 ggggaaaatg gtaatacatt gataggtaca cttggttcta atatagataa aaagccgata
      421 attttttcag gacacatgga tactgtcttt gaaacaggta catttggaga gaatccattt
      481 aaaataatag aaggaaaagc atatggccca ggtgttttag atatgaaggg gggtataata
      541 atctctttat acgttattaa agctttaaat aaaataggat acaaagaaag accaataaaa
      601 atagtatttt ctggagatga agagataggt cataaagatt caactggtgc agatgttata
      661 ttgagggaag caaaaggtgc tctttgtgct tttaatatgg aaactggatt agtagataat
      721 agtttatgta taggtagaaa aggaagaata ggatgtaata tacatgtaaa aggtgtagaa
      781 acacatgcag gtaatgattt tgaaggtgga agaaatgcta tagaagaaat ggctaataag
      841 atactacgca tacaaaaatt gactaattta gatgttggaa ctacagtaag tgtaagtgtg
      901 attaagggtg gaagagtgga aaattctatt ccagaagatt gtagtataaa agtagacctt
      961 agatttgaaa aagtagaaga aatggagaat gttaaagaac aagttagaga gatatgtaaa
     1021 gaaacttaca tagaaggaac ttctacagaa gttaatttta taagtgaaat gatgccattt
     1081 gaaactacag aagatgttat gagatttcat acatttgtaa atgaagtgtc aagagaaaat
     1141 ggatttggag aattaaatgc taagagactt ggaggtagtt cagatgcaag ttatttaact
     1201 attgcaaacg ttcctacaat ctgttcattt ggtgtaagag gagaatggaa ccacacatca
     1261 agagaatatg cagtagtaga ttctatgttt gagagggtga aattaatttc tacagttatt
     1321 ttaaacttag ataaatttga ggtataaagg gggattttat atggatgtaa cagctggaga
     1381 taataaggta acatggaaag ggtatttatc tttatttgta ttgatagtat tattttcagg
     1441 agtatttaaa gatagtcaag gcttccttaa agcattagat tttgctaatc tgacaggttc
     1501 atttggtact attgcagatg gtgtagactt tatgggtaaa ggaggaacgg gagcaaaaga
     1561 tggtttctta tttgctctga ctttgattcc aactgtctgt tttgctgtag gtcttataga
     1621 tgtagttgaa tctatgggag gaatgaaagc agcatcaaag atatttaatc cattacttag
     1681 acctctgctt ggtatacctg gaatagcagg aatagcattt gtatcttcat ttactagttc
     1741 agatgtagcc tcaataatga cgaaagaatt atttgaaagt ggggaaataa cagatgatga
     1801 aagaactata tttgtagcat atcaatatgc tggttcagct gttatactga acacaataaa
     1861 tactcaagct ccacttttac caatagcatt attggcatta ggtccaataa tccttataga
     1921 gatattctgt aaaatacttg gtgctaatct agttagatta atcataggtg taagaaataa
     1981 aaagaataag gctaaggagg ctgtgtagta tgagtcaaca aaatactgga aatgcaattg
     2041 taaatagtaa acctaagaaa actgctgttg agatatttat ggggggagca aaaaaaggtt
     2101 tttacatagg tgttgaacag atattaccag ccatgataat gggttatgta atagtgcagt
     2161 ttttacaatt aactggttta gttgatattt taggaaatat ttttggtcca gtaatgggta
     2221 tttttggact acctggggaa tctgttgtag ttttgatagc agcattcttt gctaaggcag
     2281 caggagcagc tacagctgca aacttatttg cacaaggtct aatcactgca ccacaagcaa
     2341 ccatattaat tatgccatgt atgttaatgg gtactctagt tggtcattat gccagaatag
     2401 tgcttgtagc aaatgccagt actaagtata gaatgttact gttggcagtt cctatatttg
     2461 atgcaatagt ggggatgttg atagtaagag ttttactcac tgttatggga ttaatgtaaa
     2521 taacataaaa ttataaccta attataaatg atatataaga attgatgaat agtaattaat
     2581 gaatagtaat tgatatatag taataaacta atatagtaat taatatgtat attaattaaa
     2641 aaagaagaga gtatctcaaa atgaactttt tagttcatga gatactcttt tggtattaaa
     2701 tcaagtatat tttcataatt tcaacaatga aa
//
LOCUS       DAEPLF010000317         2718 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.318, whole genome shotgun sequence.
ACCESSION   DAEPLF010000317 DAEPLF010000000
VERSION     DAEPLF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.318"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..1402
                     /locus_tag="KR314_003492"
     CDS             <1..1402
                     /locus_tag="KR314_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBH0329844.1"
                     /translation="LVNTVLISGFIGKIQIPEYILDYIKSDIILNIIYMIFLLIIYIS
                     VIRWIFSIHEITLNTENFKRARKESVNLIKRKIIRTIIYSLILSLVVAIIGYAIYHSG
                     IIFIGIWTKYCTDMEFLKNVFINRCIAFEEYFRLILSIFIFILDIGFISATYYQYKGV
                     NISKNNIKKEKKSISKIVFNLVLILLISYIESVAFSSHINGMFNTSFSFYTTAIAHRA
                     GAEMAPENTLAAIEEASNAKADYAEIDVQETKDGELIVLHDSNFKRTTGVDKNVWDVN
                     YNEVKTYDAGSFYYYGKGYYDEKFVGEKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGD
                     IVKKVVQLIKENNFENQCIVASMDKTILQKVKKLDENLLTCYFMAIAYGDFSKLNYVD
                     IYGIESTFVNKTVVEKIHKMNKKIFVWTVNSDSLIEKMLDLNVDSIITDNPYLIESAI
                     YWKKNGFISQVSDYLF"
     gene            1724..2308
                     /locus_tag="KR314_003493"
     CDS             1724..2308
                     /locus_tag="KR314_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBH0329845.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKDKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNVKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         1068 a          253 c          425 g          972 t
ORIGIN      
        1 attagtaaat acagttttaa tttcagggtt tataggaaaa atacaaatac cagagtacat
       61 attagattat ataaagtctg atataatttt aaatataatc tatatgattt ttcttttaat
      121 aatctatatt tcggttatta gatggatttt tagcattcat gagataactt taaatacaga
      181 aaattttaaa agagcaagaa aagaaagtgt taatcttata aagagaaaga ttataagaac
      241 aattatttat tcattaatat tatctttagt agtagctatt attggatatg cgatttatca
      301 tagtggaatt atatttatag gcatatggac taaatattgt actgatatgg aatttttgaa
      361 gaatgtattt ataaatagat gtattgcatt tgaagaatat tttagattga ttttatcaat
      421 attcattttt atattggaca ttggatttat ctcagcaact tattatcaat ataaaggagt
      481 aaatatttct aaaaataata ttaaaaaaga gaaaaaatct atatcaaaaa ttgtttttaa
      541 tttagtatta atattattga tttcttatat agagtcagtt gcattttctt cacatataaa
      601 tggcatgttt aatacgtcat tttcatttta tacaacagca attgctcata gagcaggagc
      661 tgaaatggct ccagagaata ctttggcagc aatagaagaa gcttcaaatg ctaaagctga
      721 ttatgcagaa atagatgttc aagaaactaa agatggagaa cttattgtac ttcatgattc
      781 aaactttaag agaactacag gagtagataa aaatgtttgg gatgtaaatt ataatgaagt
      841 aaagacatat gatgcgggaa gcttttatta ttatggaaaa ggttattatg atgaaaagtt
      901 tgttggggaa aagataccaa cactcaaaga aatgataaaa tattctaaag gtaaggtgaa
      961 attattaata gaaataaaac ttaatggaca tgaaaaggga gacatagtaa aaaaagttgt
     1021 tcagttaatt aaagaaaata attttgaaaa tcaatgtata gttgcatcta tggataaaac
     1081 tatattacaa aaagtaaaaa aactggatga gaatttactg acatgctatt ttatggcaat
     1141 tgcatatggt gatttttcaa agttaaatta tgttgatata tatggaattg aatcaacttt
     1201 tgttaataaa acagtagtag aaaagattca taaaatgaac aaaaaaatct tcgtttggac
     1261 tgttaatagt gacagtttaa ttgagaaaat gcttgaccta aatgtagata gtattattac
     1321 agataatcca taccttatag agagtgcaat ttattggaag aaaaatggat ttattagtca
     1381 agtgtcagat tatttatttt aaatattcta taattgtatt atccagtaaa agagatatta
     1441 ttaaaatcaa taatatctct ttttaacata aataaggttg ttaaattaag gttattctta
     1501 taaaaattgt gcttatcagc tataaacaag gaataaaagt gaaaaaattg ctctgagatt
     1561 attacagtta aaagagtttt aagatgacag agagatagat tgtcaactgt atatttaata
     1621 aatcttgtta tttattaaat tttatataag taaaatatat aaattaataa atcttatgct
     1681 agaatatata tgaaaaacaa tataaatttg gaggattaaa aacatgacag agcgagaaaa
     1741 aatgttggca ggagaacttt acgattgtgg tgatattgag ttgttaactc aatggcataa
     1801 agcaaaagac ctagtaagag attataacca aacagattcc tcggatttgg aaaaaaaaga
     1861 aaaaatatta aatgaattac ttggtggtaa ggataaaaac ttatggatta ctgcaccgta
     1921 ttatgtagat tatggaaata atatttattt tggaaataat tgtgaagtca atatgaattg
     1981 tacgttttta gatgataata aaattatcat tggggataat gcactaatcg caccaaacgt
     2041 acaaatatat actgcatttc atccaactaa tgctggtgat agatttggta atgtcaaaga
     2101 agatggttca tttgaatttt gtaaaactca aacagctcca gttactattg gagataatgt
     2161 ttggattggc gggggtgcta ttatcatgcc aggtgttact attggagata atgtagttat
     2221 aggtgcagga agtattgtta caaaagatat tccaagtaat atgattgcat acggaaatcc
     2281 atgccgtatt attcgagaaa acaaataaag ttaaattttc ttgcaaaatc tcaaaaaaca
     2341 cctttcttac tttatacata gggtattttc caatactttc acatccatat tcatttgtta
     2401 aatttttgtg gaatgtagtt tttatcactt ctatcttaca tttccaccat tttttataaa
     2461 agtcattcct ttatagattt atttataata gtatatattc tatttgatgt ttgaataagc
     2521 acttattttt aagtaaaata ctaactttta ggttagtatt ttgctatagt atgatgatta
     2581 agtaagaata aaatactata atacataaag cttaataaat ataattttta ttttacttag
     2641 atattttctt ttgattagta atcaaaagtg gttataattt ttttaattaa taagagtaat
     2701 atttatttta tgaaagag
//
LOCUS       DAEPLF010000318         2667 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.319, whole genome shotgun sequence.
ACCESSION   DAEPLF010000318 DAEPLF010000000
VERSION     DAEPLF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.319"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..334
                     /locus_tag="KR314_003494"
     CDS             <1..334
                     /locus_tag="KR314_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBH0329846.1"
                     /translation="ESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDE
                     VDKIYKTLENYKKDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIEN
                     KILNIIKK"
     gene            complement(457..1245)
                     /locus_tag="KR314_003495"
     CDS             complement(457..1245)
                     /locus_tag="KR314_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0329847.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVDDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            1613..2569
                     /locus_tag="KR314_003496"
     CDS             1613..2569
                     /locus_tag="KR314_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBH0329848.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVDIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
BASE COUNT          975 a          379 c          316 g          997 t
ORIGIN      
        1 agaatcaaaa atctttgatg caaaggactt taactatttt aatgatgtag aggaagacat
       61 aattcaaggt tttgtaatta attcttctga aaaattatct ctagaagata ttatcgttat
      121 aaaaactgac gaggtagata aaatatataa aactctagaa aattataaaa aagatatgat
      181 acaagcacct tttggagaag gacatggaga aaaaatcaat tctgaaatag caaataatac
      241 tataattgag aaagcaggta gatatgttta tttaatttct gctgaaaatg caactcaaat
      301 agaaaacaaa atactgaata tcataaaaaa ataaatatct attattgatg tttattaaat
      361 atcaatgtaa ttttcgcata aataaactta tttatcaaaa ttacattgat atttttttga
      421 tttcttcaaa ataatctttt tcatctatac atatgactat ccaattttta tgacctcttt
      481 tatcttatct acaacgccat tttcagtatt tctttttgct ctaaatctag caatttgttt
      541 caaatgttca tttgcatttt ccatggcata actatgatag gcacttttca tcatttccac
      601 atcatttaac tggtcaccaa ataccatagt ttcttcatat tttatatcta acattttttg
      661 tacttttttt atggcactcc ctttatttgc accttttgca gttaaatcaa gccactccac
      721 tccagaaata catacttgag ctctatcaga aaacttttca aagtagatat tattattatg
      781 ttctgcacct ttgaagtcaa atacagctat cttcaatata tcgccttcta ctttcgtcaa
      841 atcgtctaca attttaaact ttgcataata agtactaacc tcttgtataa acgcttcatc
      901 tttgctttct atataagcgt attttttccc actcattaca atttttttcc cttttatact
      961 tcttgttgtc tctatgattt catttactaa ttctttattt aaaggatttg ataatatttc
     1021 ttttccttta tatactacta gtgttccatt ttctgatata aacatcatat catcttttat
     1081 atcttcaaat ttttttataa gattttgata ctgcctgcca ctagcagcag aaaacattat
     1141 ccctctctct cttaattttt taaatacttt ataaaactct ggatttattt cgctcttttc
     1201 atctaacaaa gttccatcta aatctgttgc tattaatttt atcatacatt tcctccccca
     1261 aattattaat ttataattat tttttattaa tttttatcgg tttgaatttt caattcttat
     1321 tatacactaa aaattttaaa ttacaattta cacctttcat aagatgctat aactacattc
     1381 aggaaaatat ttttttcaat atacacatca gtttataaaa atttaagtct tcatacaatt
     1441 atattccaat aaaatcaata ataactacac actttcttct ataaaatact ataaaaataa
     1501 ttatattatg acaaacttat gctaatttat tgtgtttttt attgcttatg taacaaattt
     1561 ttaatataat taactatgtg taattaaaaa atttagaaag taggggatat tattgtcagg
     1621 actcaaaaag ttcgttatat ttctagccct tttagttata gcatttcctg ttagtatata
     1681 tggatatgtt tttactaaat taaaaactat acaagatgat agtataaata cagaggcgct
     1741 tagtagtaat gactataaaa acgaagaaaa tatcacaaac atattattgt taggtacaga
     1801 tgcaagacca ggagaagaag tttctcgttc tgatgccatg atgatactta ctattgataa
     1861 taaaaataaa agtcttaagc taacttcatt aaatagagat acttatgttg atataccagg
     1921 acgtggagaa caaaaattaa cacatgccta cgtatatgga caagctaact tattaataga
     1981 aactatagaa gaaaacttcc aacttgatat acaaaactac gccgtagttg actttttctc
     2041 ttttatggat atagttgatg ctttaggtgg agttactgtt actgttgata agagtgaaat
     2101 aagagaatta aataaattta ttcctgaaac ttataaatgg aataaaaatg acaataaagg
     2161 ttctcttaaa tatataagaa attctggtga gcaaaaatta aatggatatc agacacttgc
     2221 attttctcgt ataagaaaaa atgatagtac agatgaaaga gatagaagac aaagaagtgt
     2281 tatacagagt ttaataaatg gtgttaagga tttaccagtc actaaatatc ctaatctagt
     2341 taacacaata cttccgtatg taaaaactaa tatgaatcct aatgaaataa tatctttagg
     2401 aaaagaactt ttaagtatag gaaatttaaa tttgaaatct atggagtttc ctctttctac
     2461 tgaaaatggt agaaaaatag gtaatgcagg ctatgtaata ccttttgagg aatatgaact
     2521 agatgcaatg catgatttca tatttaaaga tattatggtt gaagattaga aattccaaaa
     2581 caaaccaata atttgatgat aaatacatac ctcttaaatg caaattgctt tgtatttatg
     2641 gggtgtgttt ttattttaaa atataaa
//
LOCUS       DAEPLF010000319         2660 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.320, whole genome shotgun sequence.
ACCESSION   DAEPLF010000319 DAEPLF010000000
VERSION     DAEPLF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.320"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(336..1154)
                     /locus_tag="KR314_003497"
     CDS             complement(336..1154)
                     /locus_tag="KR314_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0329849.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNNTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDSVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            1322..1903
                     /locus_tag="KR314_003498"
     CDS             1322..1903
                     /locus_tag="KR314_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0329850.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            1900..2094
                     /locus_tag="KR314_003499"
     CDS             1900..2094
                     /locus_tag="KR314_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329851.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2258..>2660)
                     /locus_tag="KR314_003500"
     CDS             complement(2258..>2660)
                     /locus_tag="KR314_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBH0329852.1"
                     /translation="IIYKNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSH
                     NFQVPFYYLSYGLASDVSLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQ
                     LPFQDGNLEEISSTLYDYFGIDNPLELKNAS"
BASE COUNT          962 a          350 c          359 g          989 t
ORIGIN      
        1 gtactttact ctttgttttg ttaaaaacta atttatatta gtgattcaaa tatttaacta
       61 gagtataagg gttgtatata atcttataag taatttatct gacaaattaa gataatatcc
      121 taatcaaatt ctagtcattc gaacataaca tcacaagtga tttatctgac aaattaagat
      181 aatatcctaa ttaaattgta gatatttata cataacacta aaagtaattt gtttaacaaa
      241 ttaagataat atcctaatta aattgtaggt atttatacat aatactaaaa gtaatttgtt
      301 taacaaatta aaatattatc caattaatat aaaaattact tctttttaac tgggaagcat
      361 acctctgtta cccaattatc aggattacta tcgttgccag gacttacatg ataaatatta
      421 aacataggac catccacatc atagttatta tctgatatcc aattaccaat agcctcacaa
      481 gcgtctgcaa cttgattgaa atttccatta acaattgcag ttgcagcagt cacacttgga
      541 acagtcttaa atttaacatg ttcagtatct ttatactttc cagatacagc aacttgtact
      601 tcaacatcta cagaatcttc tttatatcca gtatcataaa agactgcttt agaatacttt
      661 gggggttcaa tttgaacatt ctggtctttt gtctctaaaa atgcttgatg ccataacatt
      721 ccttcgtctt gataagttgg tataaccctt ctaagtgtca tcatatattt tggtgcaaaa
      781 ttctttatag ttacatcata atttttcata atatcatttc ctccaattct tttaatagtg
      841 gtttctattt ttaatagttt tttctgtgta tcttctaatt gttcttttac ttgagaatgg
      901 tggatattta gatattttat aagactttct ttatcgttat attctgtcaa aatttctttt
      961 atagaataca agccaaagcc catatctttt agtgcatgga ttctatttgt agtagaaagt
     1021 tggtctgcac tatagtatct atatccatta gtgttattcg tatgagaagg agttaaaagt
     1081 cctatttcat cataatgacg taacatacga atactaattt tacttagctt tgaaaaatcg
     1141 cctatcttaa acatgttttt gtctccttta taaaggtact tttagcttaa tattaatata
     1201 aaccatatca taatgttaga gtcaactaaa attataatta aattatattg acatatgatg
     1261 aaaaaaatat tatactgaac atgttcagaa cctaaaatgt taattatttt ggaggtctat
     1321 tatgaaaagt aatgataaag atgctagaga acgtataatt gaagttacat tgaatctttt
     1381 aaatgaagta gatgatattg aggagattac agttagaaaa attgctgagc gtgcaaatgt
     1441 tggagttggt ttaataaatt atcattttaa aacaaaggat aatttgttga gtactgccat
     1501 aggagatgtg atgtcaaata taatagcaga gctatatgac gatagtgtat atactctaag
     1561 acctattgaa gatttgaaaa atttattaaa aaaattgtgt gatactggat tacattatga
     1621 aaaagtgctt ccttttgtgt tgaatcaatg tataaccaat ggagatatgc aagcagaact
     1681 tgatattgtc cctatgcttc gtaaaatttt tggaaataaa aaagatgaaa tgagtctaag
     1741 aatcattgcc ctgcaaatta ttttaccaat acagatttct gctttatcta cagaatcatt
     1801 tcagttatac agtggtataa atataaagaa taaatatgag cgagataaat tcattgatat
     1861 tttaatagaa aatattattg gagaggatgt tgatgtaaga tgaaaattat aactattggt
     1921 tcaagtctta tcactgtttt actttttctt tcaactatgg tttgtgggtt ctggattaag
     1981 aataataagg taacagatgc aagttctatt aagttccata tgaatagtgc tatttttaca
     2041 gggatatttt tattgatttc cacaatcctt ttaattatat atataaaaaa ataataatat
     2101 tattaataaa taaagtatca gaaattataa agacaagagt cttaaaaatt tctgatactt
     2161 taccacataa ttttattaaa cattaattgt gtgtgaaaaa atatcactta tttcaatcta
     2221 atactaaatc ttaagagtga gtataaagta ttagaaatta agaagcattt tttagttcaa
     2281 gtggattatc tataccaaaa taatcatata aagtagagct tatttcctct aaatttccat
     2341 cttggaaagg taattgtaaa tttacctttt ctaccacatc tttaaatcca gcatcagtat
     2401 tttgatttaa aaaatccata tatacatcaa cagcttttct gtaatcttct tgtgataatt
     2461 gccatatctg aagtgataca tcagatgcaa gaccataact caagtaataa aaaggaactt
     2521 ggaagttatg agatacaagt atccaaaaag gagcattttt ttctctaagt atattattgg
     2581 aaactccata ttcttcctct aaatcaaaaa acaatttatt gatatcattt agactcatat
     2641 atggattctt gtatataatt
//
LOCUS       DAEPLF010000320         2656 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.321, whole genome shotgun sequence.
ACCESSION   DAEPLF010000320 DAEPLF010000000
VERSION     DAEPLF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.321"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            168..836
                     /locus_tag="KR314_003501"
     CDS             168..836
                     /locus_tag="KR314_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329853.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLASNFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            838..1323
                     /locus_tag="KR314_003502"
     CDS             838..1323
                     /locus_tag="KR314_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBH0329854.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1384..2505
                     /locus_tag="KR314_003503"
     CDS             1384..2505
                     /locus_tag="KR314_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBH0329855.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMQFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
BASE COUNT          993 a          296 c          532 g          835 t
ORIGIN      
        1 tatattttta aacttgaaaa gtaagtaatt ataagctatt taatcgctgg aataaattta
       61 caataaaaaa ataaatacaa aggctttatc attaaggtat tggcataagt tttgcttatt
      121 aaacaagtat actaataaaa ttcatttaaa caggagggag aatttatatg agtaaattag
      181 aatcaacaac agaaaaagtt gaggtaaaat ggtatgggta tgtagcatta atattgggag
      241 cattattttt ttcaggaata tttaaagatg caccaggacc attaaaagta ttggatttta
      301 acaatgtatt aggttctttt ggaacattgg gaacaattaa tgatggtgta ggtacattag
      361 cttcaaactt tagaggtgat ggaggtactg gccctcgaga tggatggcta tatgcattga
      421 cattaatacc atcagttatg tttgcacttg gagtagttag agttatagac cacttagatg
      481 gaatgaaggc agctcaaaag cttcttagtc cactattaaa gccattatta gggttacctg
      541 gatttgcagg actaactttg atagctagtt tacagtctac agatgcagct gcatctatga
      601 ctaaagaact taaagatgat gggtatatag atgaaaaaca aaaagctgta ttttgtgcat
      661 ttcaattttc aggtgcgtct gcaattacga atttctttgc ttctggagca gcactatttc
      721 catttatagg agatgtacca atatttatac cacttgcttt aatattaatt atgaagtttg
      781 ttggggcgaa cttacttaga ttatatttaa ataaatttga caagggggaa gcttaacatg
      841 agtgaggtag caaaaaaaga agtaaaagtt aaaaaaggaa atgtgataga tgcgtttatt
      901 ggaggagcta gaaatggatt ccaaataagt acaaattcaa tggctccaaa tgtcatcttc
      961 ggttttgcta taatttctgt gttcaattta acaggtcttt tggatgtaat agggacaata
     1021 tttacacctg taatgagtat ctttggactt ccaggtgttg cagcaacagc tataatgaca
     1081 atatttatat caatgggagg agcatctgga gtaatcgcag gtttatttac agcaggtcaa
     1141 ataagtcaag cacatgttgc aattctattg ccaatgttat tgctttcagg agcgcagata
     1201 caatttatgg ggagactttt gggaacagct gagttaaaaa ctaaatatta tccacatatg
     1261 tttgttatag caactttaaa tggttgtatt gcaatgctta taatgaatta tttcgtagta
     1321 taggtaatta taataaaaat ttaatataaa tggaaagttt aaattttaag gaggagctag
     1381 attatgggaa ctaacaaaga ttatattgag tatgtacata aagtgtatga ttatctacac
     1441 actataccag aaaaaggatt tgaagaatat aagacatcag agtttgtttt aaaagagctt
     1501 gaaaaaatgg ggtttgaaaa tattaataaa ggagttgcag gaactggtat tatagtaact
     1561 atagattcta aaaaagaagg accagtattt gctataagag cagatatgga tgcattggaa
     1621 tttatcatag atggagaaaa gaaaatgata catgcttgtg gtcatgatgg tcatacttct
     1681 atgcttttaa cagctgcaaa gttaataaaa gagagaaatt tagtaaaaag aggtaagtta
     1741 aaaatacttt ttcaaccagg tgaagaaaaa ttatatggag cattaagagt aattgattct
     1801 ggtttgttgg atgatgtaga agaaatggta gggatgcatt taagacctgg acaagattgt
     1861 aggttgggaa aggcgatacc tgcaatgtac cattgtgcta gctgtataat gcaatttacg
     1921 attaaaggta tcacttgtca tggttctaaa ccacatttag gagtaaatgc agtagatgct
     1981 gtctgtttag cagtgaatgc aataaatgca ataagagtaa atccagctat accaaattct
     2041 ataaaagtaa caaatatatc agttaaggga gaaacttata ataatatacc agatgaagca
     2101 tttatggctt tagatataag ggcagaaagt aatgatataa tagaagaatt aaaagaaaaa
     2161 gcaatacatg ctgtaaaaac tgctgtaagt tctataaatg cagaagctgt actaatttcc
     2221 aaagatggag taccagcagc agaatttgat gaagaacttg taaacttggc tgaagaaact
     2281 ataaaagatg tattgggagc tgatgcatct ataggtatat ttaaaaattc tggtggagaa
     2341 gatttccatt attatacaca aaaactaaaa tgtaaaacta catatattgg attaggagct
     2401 gatgctacac caggatttca taatccaaat gtaacatttg atacgaaagc tttagaatat
     2461 ggtgtagata tatggtgtag attggttgaa aagagattgg gataatatcc aaactaataa
     2521 ttctacattt aaaattataa ttatagaagt tatagaaaaa gattgcttat gttaattaga
     2581 aaataattaa tatgagcaat cttattttga tataatgaaa ctgattttgg aatattgaaa
     2641 aaatatgatg gaaaag
//
LOCUS       DAEPLF010000321         2646 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.322, whole genome shotgun sequence.
ACCESSION   DAEPLF010000321 DAEPLF010000000
VERSION     DAEPLF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.322"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..489)
                     /locus_tag="KR314_003504"
     CDS             complement(<1..489)
                     /locus_tag="KR314_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0329856.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHIIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNVIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVF"
     gene            677..1582
                     /locus_tag="KR314_003505"
     CDS             677..1582
                     /locus_tag="KR314_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBH0329857.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            1591..>2646
                     /locus_tag="KR314_003506"
     CDS             1591..>2646
                     /locus_tag="KR314_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0329858.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     RIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQL"
BASE COUNT         1048 a          238 c          439 g          921 t
ORIGIN      
        1 gaaaactata gaatttaaat tagattcttc ttgtatatat gatggactta atacatatat
       61 atttatattg ttatctatgt ttaaaacatc atttttatag agatgctgaa tagataagtt
      121 tttatccgta caagaattta ttagattttg ataagcctca ctatcagttg attgttcagg
      181 catatatatg gagtttacat taaatttttt tataacatta tcaagacttc ctatgtggtc
      241 tgaatctgga tgagttgcta ttataatatc aatagttttt attctttttt gtctaagata
      301 actaataatt atattttcgc tatcttcatc acctccatca attaatatat tcttatttgt
      361 gggtgtttga actaaaatac tatcaccttg tccaacatca attatatgta tagatagtag
      421 agattttttg tcacagccga ttaaaagtga tattataata agtattgata aaaaaatttt
      481 atttctcaat aaaaaatcac tccattcatt acatgtttat ataaatttat gatgggtaat
      541 ataagatata tatgacttat tatattttat attttaaaca atataaatga aattaatacc
      601 ataataaatt tggagttttt tatatataca aaataaaatt tttaaaatta agcaataaaa
      661 aaggtaggag gattttatgt ttttcaaaga tattgaacta aattcaaaaa aagagttaga
      721 cccatatttt gatttagtag attatgaagc ttgtgagtac tgttttagta ctttatatat
      781 gtggcaacat gtttataaga caggatatta tataggtgaa gattttgccg ttttagttgg
      841 ggaatacgaa ggagatagtt tctcaatatt accacttgct aaaaaagata aacttccaga
      901 agttgtagat tttgtccttg aatatttttc taaaaataat aaaaaaatat acttaagagg
      961 tattactact gaagtagtag agtttttaaa agaaaaatat ccaggtagat ttgagtatat
     1021 agaagaaaga gatttatttg actatattta tgatgcagaa agtttaagaa ctctagctgg
     1081 taaaaagaat caaaagaaga gaaaccatat aaactatttc ttaaaagagt atgcaggtag
     1141 atatgaagct aaattattag ataaagaaaa ctttgacgag tgtttagtac taatgaagga
     1201 atgggaaagt aataaggaag aaaataatga atttgatgaa agtatggatg acgaattaat
     1261 aggtatcaaa aagatattta accattatga catattaaaa gataaggtaa aagtgtttgg
     1321 tgtttatgtg gatggaaaat tagaggcttt tagtatagga gaacttttaa atccaaatat
     1381 ggcattgatt catatagaaa aagctaatcc cgatataaga ggtctgtatc catttataaa
     1441 tcaacaattt ttggtaagtg aatttaagga cgttgagttt gtaaatagag aagaagattt
     1501 aggtattgaa ggattaagaa aagctaaact ttcttatcat ccttgcagat ttgttgaaaa
     1561 gtacagtgtt agggaggctt aatagactat atggagatta ggtatgctaa agaagaagag
     1621 atagaaagta taaaagagat ttggagttat tgttttaacg atacggagag ttttatgaag
     1681 tactatttta atgataaata taaatctgaa aatactgtag tagctttaga tgaaggaaaa
     1741 attatttctt ctcttcagct aaatcagtat aagttattac taaatagcaa ggtatataat
     1801 acatcatatg tagtaggagt ttctacactt ccagagggaa gaggagctgg atatatgaac
     1861 aaagtaatga aatttacttt aaacgaattg tataaaaaag ggcaattagt ttctatattg
     1921 atgcctattg actatagact ttatagaaga tttggatatg agcattgtta cgaccagatt
     1981 gaatatacta ttaacacaga tgatttaaaa aattttaagt caagtggaaa aatgataaag
     2041 tctaatttat cacaaattga tgatttgata cgaatagata gaactttttt aaatgaggtt
     2101 aatggtaatg tgttaaaaga tgaacattat tatgaaaact tatttaaaga gattcaaagt
     2161 gaagatggat ttttatacat tcatgaagga aatgaaaaag atggatatat agtttatttt
     2221 cttcaggaag ataaaatgtt tgtcagagag ttattttata aaaatataga tgcccttaaa
     2281 tcaatgctta aatttatata taatcacaat actcaatgta agatagttac tatatctact
     2341 cctacaatag ataagataag atttatatta gacaacccta aagatagtga tataaaaata
     2401 aaacctttta tgatgggaag agttataaat gtaaagaagt ttattgaaga tatagatatt
     2461 gagaaggata ttaatagctc atttaattta ttaatagaag acaagtttat agatgaaaat
     2521 aatggtctat ttaaaatttc tatacagaat aagaaggtat ctgtagagca gttagataaa
     2581 aaaggtgcag aaaagcctca agaagatttt gatattaaat tagatataaa taccttaaca
     2641 cagtta
//
LOCUS       DAEPLF010000322         2601 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.323, whole genome shotgun sequence.
ACCESSION   DAEPLF010000322 DAEPLF010000000
VERSION     DAEPLF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.323"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     regulatory      156..253
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            308..484
                     /locus_tag="KR314_003507"
     CDS             308..484
                     /locus_tag="KR314_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329859.1"
                     /translation="MFGNKMDKCTHVLTAYISGSYDYCNFLDTQLDDFILEYGESVVE
                     SCLHQVMVLVSKYN"
     gene            710..910
                     /locus_tag="KR314_003508"
     CDS             710..910
                     /locus_tag="KR314_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH0329860.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(1074..1373)
                     /locus_tag="KR314_003509"
     CDS             complement(1074..1373)
                     /locus_tag="KR314_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329861.1"
                     /translation="MGFYINYVVCKVKILFSSFSKIILFYINYVVCKLFITLKAILLK
                     TQIIVTTHSPHILQIDSKEEMIVLDMAESGNVYKKELELGEYGVLGWTNEGLYLH"
     gene            1595..1777
                     /locus_tag="KR314_003510"
     CDS             1595..1777
                     /locus_tag="KR314_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBH0329862.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            1848..>2601
                     /locus_tag="KR314_003511"
     CDS             1848..>2601
                     /locus_tag="KR314_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329863.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINNSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDE"
BASE COUNT         1017 a          280 c          366 g          938 t
ORIGIN      
        1 agaacaaaaa ctatagttaa tttttaccag gtactctacg tgtttagtct gttttttaag
       61 tgtaaaataa tgttcatatc tataaattta tttaactttc aaacaatcct gttgaataat
      121 aactaattct attgtattat tttagttgta gaataaaact aaatcggcaa aactagagaa
      181 atttagtgac gcaaagctat agggactaag acttatataa atatcttatg agttatgtca
      241 gccagttgcc aaagagatag tgttcttttt gtttttatga aagtttttta gggggataat
      301 aatttatatg tttggaaata aaatggataa atgtacacat gtattgactg cttatattag
      361 cggttcgtat gattattgta attttttaga tacacaatta gatgatttta tattagagta
      421 cggagaaagt gtagtagagt cttgtttgca ccaagtgatg gtattggtaa gtaagtataa
      481 ttagatagac attgatttct agagaaccct ttttagtttt atctacattt atattaatat
      541 atagcaatgt aaaacgctta aatatatttt taatattaag aataactaaa atgttgacta
      601 aaaaatataa ttaagtagac atttctatta atctatgata caatagtatt tgtggtaata
      661 cacaattaaa tcaagtatat cttgatatag gttaatagga gtatttttaa tgaataatgg
      721 aatagtgaaa tggtttaata atgaaaaagg attcggtttt atatcaatgg aaggtagaga
      781 tgatgtgttt gctcatttct cagctataca aacttcagga tttaaatcat tagaagaagg
      841 tcaacaagta agctttgata tagttaaagg tgctagaggt cctcaagcag aaaatataac
      901 tatattataa tatatagatt tttcgggaaa gactcttttt agagtctttt tttattgtta
      961 gtatatgtaa gataatcatt ggtgaagtaa ataaggtata ttgagtaatg ttcttgatag
     1021 tttatatgct aatgaatttc aaaaaaataa gcactcctat aaaaagagtg ctcttaatgt
     1081 aaatataagc cttcattggt ccagcctaat accccgtatt ctccaagttc taactctttt
     1141 ttatatacat taccactttc agccatatct aacacaatca tttcttcttt agaatcaatt
     1201 tgtaaaatat gaggactgtg tgttgtgaca attatttgtg tttttaggag aattgctttt
     1261 aatgtaataa aaagtttaca tacaacatag ttaatataaa acaaaattat ttttgagaat
     1321 gagctaaata aaatcttaac tttacatacc acatagttga tataaaaccc caaaataaac
     1381 ttagcatctc caatgtttac acatatacaa tgctcttaaa attgcagtga acatgcaata
     1441 gtgcaattga taatacttat catatattct caatacctta tatttcaact gctaaacctc
     1501 aatttgtcac aaatatcgct cactgcaaaa tttctatatt tttattatat catagcatat
     1561 aattaatatt ttcaattaat ttttacattt aattatgttt aaattctata ataatgcaac
     1621 tttaatgttt ctttctggaa ctaatataaa aactatttca gaaagattag gtcatgcaga
     1681 tataaaaata actatgaata gctactctca gtttttagaa gagatggaca gagaagcatt
     1741 tgaaaatttg agcaaaatac tatttattgt attttaaaga agatgactta ttcaatattt
     1801 gatgctagaa tataattaat aaaaatttaa ttataggtga ttgtattatg aacattatta
     1861 aaagcataac tttactctta acattattaa ttgtgatatt aatgacagga tgttccccaa
     1921 gagaaagtgc tagtgaagtt gcaaaaaaat atattgataa gatgaactct ggtgactatg
     1981 aaacagcata tagtttatta agtaaagata gtaaaaaaaa aattagtata gaaaatcttg
     2041 aagaatttca aaatatttta gttagtacaa aaagaataat tagttataaa ataggtaaag
     2101 aaaaaatcat aaaaaaatat aaacatgatg gaaaagaata taaaaatgtt gttaaattag
     2161 aagaaacttt taatataaaa aaccttcttt ataaaaaaga tgattctcta aaagtaaata
     2221 gatattttgt agttgaaaat aacaaataca agctattatt gtatgaaagt ttcaaaaaag
     2281 aatttggtat taatagtatg gaccttgctc aacttaagct taatgatttt ggactaaaat
     2341 actttaaaga agttgacctt atactaaaaa aagctattgc tattaatcct actgattcaa
     2401 agctttatta tgcccaagca tgtaattata tgaattttga tggatttcct ggttattcag
     2461 atacaacata taatgcttta gattcaatta ataattcttt aaagtattta gataaaaatg
     2521 attctgacta caaaaaaaat gcaagtaata tatataactt aaaaggagtt attcttatgt
     2581 ttcacaaaag atttgatgaa g
//
LOCUS       DAEPLF010000323         2594 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.324, whole genome shotgun sequence.
ACCESSION   DAEPLF010000323 DAEPLF010000000
VERSION     DAEPLF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.324"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..420)
                     /locus_tag="KR314_003512"
     CDS             complement(<1..420)
                     /locus_tag="KR314_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0329864.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGA"
     gene            complement(451..>2594)
                     /locus_tag="KR314_003513"
     CDS             complement(451..>2594)
                     /locus_tag="KR314_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0329865.1"
                     /translation="NEIDFFSKIPNREFMYQYIDDCISKNIHIGIMLLNIDNFKLFND
                     IYGYLVGENLLITLTQNLLHFIPESKGIVAYLKGEGFLIVLPNVSNKELNNMGATIIE
                     SSRELKIRLPNEKEIYSNVTFSIGSTIWNGEPNMTTLILFNQVYSALKKAKSEGKNRY
                     ILFDFKDNSFYNKLENDKEFLSEISHVLQTKQIEVFYQPLYNIKNNTIVGCEALARWR
                     HPIKGLLTPDKFIPLFEQFGLITELDLHLFEENCKNIRRWIDNNDVFVPICCNFSRLH
                     FFNPNFPSMLKGITEKYSVSTSNFSIEITENILIENTNTIIQQLAELRSYGFSVSMDD
                     FGSGYSSFGMLQNLPFDIIKIDRIFLSHNLNDFKNTTILYSIINIARALGMLTVCEGV
                     ETEQHVEFIKSINCDIAQGYYYAKPMECKEFEKLLSKENNSEKMFIKYSDIGKSLIEK
                     IFDGFFIMQDMKKLRANVTEDIQWFDFYNKTEITSFPKVIEHFEKHIKGKKFSILYKS
                     ITPHNTESDSLLISGEAVLSDEASEHQKYSNFYFSANCIISENKLLLAKLHLDLIKTA
                     GYINEFESYSIPKMTNTHLDESNILSQFYSMLPVGIIRYDLFGDMIITYMNEEMFDII
                     GYTKEQFFGEMGGNLRAIVHPDDLDFVYKKSLDMIDTNKIEPFLYRFIKRDGQIVTVM
                     YTQCNLSAIDGRSIAQGMYVDVNKIKNLTKI"
BASE COUNT          888 a          370 c          314 g         1022 t
ORIGIN      
        1 tgctccagtt aatgggtcag taattgagta gtgaagattt ctcttatttt cttcatagag
       61 ttttgttata tcattggcca tagtaattat gtaggttttt tcatcaacat ctactttgta
      121 ttctaaaaca ttccaatagc ttgtagtatc ttttttaaac tctaaaatcc aatttgaaat
      181 tttatcataa ggataatttt tttcccaatc taattgaata ttgtgagaat ccgtaaaaaa
      241 tttagcaata ttatcaggga gtttcttagc tatttctata ttagtcattc caagtggatt
      301 atcttgcata taacttttaa ttaaattttt taaattatca tttataaata taattctgta
      361 gttagaatct cttataatta gaaatcctgg aaaagttctt aaaaaaattt ctttaaacat
      421 attaatttct ccaaaataaa atactttgta ctagattttt gttaaatttt ttattttatt
      481 tacatctaca tacatacctt gggcaatcga ccttccatca attgcagaaa ggttacattg
      541 tgtatacata actgtgacaa tttgaccatc tcgttttatg aatctatata aaaatggttc
      601 aattttattt gtgtcaatca tatccaaact ttttttataa acaaagtcta aatcatctgg
      661 atgaacaatt gctcttaaat ttccgcccat ctcaccaaaa aactgttctt ttgtataacc
      721 tataatgtca aacatttctt cattcatata agtaataatc atatcaccaa ataaatcgta
      781 gcgaattata cctacaggca acattgaata aaattgtgat aaaatattac tttcatcaag
      841 atgtgtattt gtcattttag gtattgaata tgattcaaat tcatttatat agccagcagt
      901 tttaattaaa tccaaatgta attttgctaa gagtaattta ttttctgaaa ttatacaatt
      961 tgcactaaag tagaaattgc tatatttttg atgttcagaa gcttcatctg ataaaacggc
     1021 ttctccacta attaataatg aatctgattc agtattatgt ggggttatac ttttgtaaag
     1081 aatagaaaat tttttacctt taatatgttt ttcaaaatgt tctattactt taggaaaaga
     1141 tgtaatttca gttttgttgt aaaaatcaaa ccattgaatg tcttcagtga cattagctct
     1201 tagttttttc atatcttgca taataaaaaa tccatcaaat atcttctcta ttaaagattt
     1261 tcctatatca gaatatttta taaacatttt ctctgaatta ttctctttag atagtaattt
     1321 ttcaaattct ttgcactcca tgggttttgc ataataataa ccttgggcga tatcacaatt
     1381 tatactttta ataaattcta catgttgttc tgtttcaaca ccttcacata ctgtaagcat
     1441 cccaagagct cttgcaatgt ttattataga gtacaaaatg gttgtatttt taaaatcatt
     1501 taaattatga gataaaaata tcctatcaat tttgattata tcaaaaggta gattttgcaa
     1561 cataccaaaa gatgaatatc cagaaccaaa atcgtccatt gatacagaaa aaccataaga
     1621 acgaagttca gctagttgtt gaataattgt atttgtattt tctattaata tattttctgt
     1681 tatttcaatg ctaaaatttg atgtagagac gctgtacttt tcagtaatac cttttagcat
     1741 acttggaaaa ttagggttaa aaaaatgaag acgagaaaag ttacaacaaa ttggaacaaa
     1801 aacatcattg ttatcaatcc atcttcttat atttttacag ttctcttcaa ataaatgcaa
     1861 atctaattcc gttatcagac caaactgttc aaataaagga ataaatttat ctggagtaag
     1921 aagtccttta atagggtgtc tccatcttgc aagtgcctca catccaacta tagtattatt
     1981 ttttatatta taaagtggtt ggtaaaatac ttcaatttgt ttagtttgta gaacatgtga
     2041 tatttctgat aagaactcct tatcattttc taatttattg taaaaactat tatctttaaa
     2101 atcaaataga atgtatctat ttttgccttc acttttagcc ttttttaaag cactatatac
     2161 ttggttgaaa agtatcaaag tagtcatgtt tggttcacca ttccagatag tgctgccgat
     2221 actaaatgta acattggagt atatttcttt ttcattagga agtcttattt ttaattctcg
     2281 ggagctctct ataatagttg cacccatatt atttaactct ttatttgata catttggaag
     2341 tacaattaaa aaaccttctc cttttaaata agcgactatt ccctttgatt ctggaataaa
     2401 gtgcaatagg ttttgagtta gagtaattaa taaattttct ccgactagat atccatatat
     2461 atcattaaat aatttaaagt tatcgatatt taataacatg attcctatat gtatattttt
     2521 cgaaatacag tcatcaatat attggtacat aaattctctg tttggtattt tagaaaaaaa
     2581 atctatttca ttta
//
LOCUS       DAEPLF010000324         2594 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.325, whole genome shotgun sequence.
ACCESSION   DAEPLF010000324 DAEPLF010000000
VERSION     DAEPLF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.325"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            277..570
                     /locus_tag="KR314_003514"
     CDS             277..570
                     /locus_tag="KR314_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0329866.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            551..1192
                     /locus_tag="KR314_003515"
     CDS             551..1192
                     /locus_tag="KR314_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBH0329867.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVILQI
                     LMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKQNYSVGIILPWTLDDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            1436..1768
                     /locus_tag="KR314_003516"
     CDS             1436..1768
                     /locus_tag="KR314_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBH0329868.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            2310..2474
                     /locus_tag="KR314_003517"
     CDS             2310..2474
                     /locus_tag="KR314_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329869.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         1005 a          215 c          397 g          977 t
ORIGIN      
        1 tttgaaatta aaagtatatg taatatatat aagtatatca gtcattaggt ttttgcttta
       61 acaattgtgg tgcaatacac ttttgttaaa ttaaaaaaat tagataaaat aagattatat
      121 aaaagcgact taagtaaatt gagaatctta atatgaaaat tttcaataaa tatttggaaa
      181 taactattta gaaatttttc taaatagtta ttgaaataaa atggatgtta tgttatattc
      241 tatttagatg aaattctaaa tagcgaggtg atggtgttgg ggtttccaga aacattcaaa
      301 gctctttcag accctataag aagagaaata ttggttatgt tgagggagaa taaaatgtca
      361 gcaggagaaa ttagtgaaaa atttaatatg acgaatgcta caatttctta tcatctatct
      421 caactaaaaa aggcaggact catatttgaa acaaagtata agaactttat tttctacgaa
      481 ataaatattt cagttttcga agaggttatg ctttggtttt cacaatttat ggagaaggga
      541 gaaaaagata atgaaaaaag cgataaataa acaatttatt ttatctactt taatatgttt
      601 tataccattt attgtatcaa tatattttta taatagatta ccaaatgaag ttgcaattca
      661 ttttgataat tatggaaatc cagataatta tgctccaaag gttatagcag cttttggtgt
      721 accattgtta atgctatgta tccatttgta tacatggttt agacttgaga atgagcctaa
      781 aaaagctaat ttttcaaatg ttatacaaaa tttatcaaaa tggggagttg ctatactttc
      841 agtaattcta caaatactga tggttttata ttcaacagga gttagtttgg agtctaattt
      901 ttatacagga ttaattttag gaataatagt tattttattt ggaaactatc ttcctaaatg
      961 taaacaaaat tactcagtag gaataatttt gccatggact cttgatgatg aagataattg
     1021 gaataagaca caccatttag ctggatggat ttggcttatt ggtggaatat tattaattat
     1081 taatgcattt attaatattc ctttttataa tatctttgta atctttgtaa ttgtaatact
     1141 accatttata tattcttatt tgatgtataa aatttcaggt aaaaataact aatataatta
     1201 atcactataa taatgtttgg ataagaataa tatatttcag aactgagata gataataaag
     1261 aaattactaa gatttctatt ttagttctga aaaaatctta cttatatgat aacttattga
     1321 gatttcaaaa aataatataa attaggctaa tgaacataaa atgaaaattt gtatttaata
     1381 ttgaaaatat aagtacagtg tgatactctt ataagaaaaa cataggaggt agaagatgaa
     1441 tactagagaa atgaaagaat ataggggatt ttacaataaa aaattaagtg aaacagatga
     1501 gttttttaga gtatttggaa gtcttgatga taagacttat tcagaagggg ctataagtaa
     1561 aaaaaataag gaattaatgg gactagctat ttcaatttta agccgatgtg atgagtgtat
     1621 ttgttatcat attgagggtt gtattaattc tggagcaagt aaagatgaaa ttatagaagc
     1681 tataaaaata ggtgtaattg gtggcgggtc tattacatat ccaaacgcaa gatttgcaat
     1741 aaaaatttta gaagaattta atatataatt ttgatacacg tagtttattt agactaataa
     1801 taatattcta tttaagtctt tgaaattaag ctttattttc taagttcact tcttattaag
     1861 tttttagaat tgcatatata aatataaatt tttaaataat atagtagttt taaattattt
     1921 acatcataga ttgtattgag aatagtatat tgtcattatt aaatctttta tacatttttt
     1981 attttaaata aattgaatat taaggtgatt atatttacat atttgtattt ttatgaacta
     2041 gaatattctt tataaattca tagcagtttt aatagataac tttttcagat agattaaaca
     2101 aagttttatt tactgtattc tattatcagt ttcctacaaa ttaaattgtc tatttttatt
     2161 tatagagtag aataaactgt ttttgttaag aattttgaag gtaaagctag tttaaaaaaa
     2221 taaagggact aatttgtcta acgtttattg gtgaaaaata agtctataga agttataata
     2281 aatatataaa aatgtatgag aggtgttata tgtttggtaa accaaaagaa agtgttagat
     2341 ttttagactt taatgatgta gcagaaaagt ttggagagaa aaaggctgtt gcaatttttg
     2401 atgccatagc tggaaaaaaa tctaaagaac atattatcaa agatatagaa aaattagtaa
     2461 aaaatgaagt ttagatattt ataaggtata ttgagcttga gggtagctct ttataccttc
     2521 ttttttatga tttttagata atattttaaa atttaaataa gaaacttatt gttatagagt
     2581 aatatttata taga
//
LOCUS       DAEPLF010000325         2582 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.326, whole genome shotgun sequence.
ACCESSION   DAEPLF010000325 DAEPLF010000000
VERSION     DAEPLF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.326"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..424)
                     /locus_tag="KR314_003518"
     CDS             complement(<1..424)
                     /locus_tag="KR314_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBH0329870.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGII"
     gene            complement(586..1803)
                     /locus_tag="KR314_003519"
     CDS             complement(586..1803)
                     /locus_tag="KR314_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBH0329871.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(1994..2407)
                     /locus_tag="KR314_003520"
     CDS             complement(1994..2407)
                     /locus_tag="KR314_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0329872.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKTEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT          899 a          395 c          296 g          992 t
ORIGIN      
        1 gtattatacc attctggtaa attgtagact caaaagttga actagtcgaa aaaagagtta
       61 ctttttgtga gatagtaggt aattttttaa ctgtttcttc tacaatattt aatattggta
      121 tattaataga ctcttttaat tctttaaagt atatatgtgc tgaattgcat ggcattgcaa
      181 taaaatttac accagttgat tcaagctttt gtaatccatc aattatagtt tctttcataa
      241 gttcatgatt tattggacgg tcaatataaa atggggtagg taatgaatat atcatcatct
      301 ttggaaattc atcatcatat tttgctccat attgagattg acactcatca ataattaaat
      361 caacaaaagg ggctgttgac cttggaccca ttccagccaa aataccaatt aatttttcat
      421 tcataaaaat tatctccttt atatataaat atatttatgt ataaaataaa aatctataaa
      481 agcataaaat tatattttat aatagattat attgtacact tgtataatat aaatataaag
      541 tgatttttta gattatatat tttaatatac tttcttatat aaaaattatt ttaaatcaag
      601 attatctagt atttttaaaa atatttttac agcagtatcc aagtcatcta cagaaacact
      661 ttcattagga ttatgactta cgcccccagc acatcttata aataccatac caatatcagt
      721 tatattatcc atttcctgag catcatggcc agcaccacta taaatataaa aaggattaag
      781 atttaaatct ataaaacttt tttctatgat ttttgttatt ttattagaac atggtacagg
      841 aacattttca aaagcaagtt cacttgtata attaagtttt ctattttcac atatatgtga
      901 tatttcatta aatatctttt ctacagaatt atcaagaatt tcttgtgaac aagagcgtac
      961 atcgagtgta aattcagctc tttcaggtac cacatttgat gaagatggat agaaattcat
     1021 ctttcctaca gtagcaacca aatcagcagt cgttttagct actttttcga tagctaatac
     1081 acattcacag gcacaacatc cagcatcaag tctcatattc attggaagtg taccagcatg
     1141 tccagactta ccatttactg atatcttaaa acttttaaat cctgtaatac ttgatacaat
     1201 tccagcagga agagattcat tttctaaaac aggaccctgc tcgatatgaa attctaaata
     1261 tgcatctaca tcttttctag gatgcaaatt tgttaagttt tttgtattaa atccaaactt
     1321 tgttacagct tcttcaagtg taattccatt ttcatcaaca gatttaagaa gttcctcttt
     1381 aaaagtacca gcaaaaactt tacttcccat taaagaagtt tcaaatctag ttccttcctc
     1441 ttcagcgaaa gcaattagct taatattgaa aggataagac ctattattgt gttttgcttc
     1501 ttcaagacca actagaggca ctattatacc taacatacca tcaaatatac ctccacactt
     1561 tacactatct tgatgagatg caactactaa tgtgggagca tttggaaagc ttgatttata
     1621 tgtacctata acgtttccaa tgtcatcaat ttcaatatca agatttaaac cttccatcca
     1681 tgaactaatt aaatcaatag attttttatg ttcttgagtc aagataagtc ttgtaagacc
     1741 atttgttgtt gaacttattt ttcctaatgt ttgtagataa tccatacatt tttttcctaa
     1801 catatttacc tcctaaaaat ataattttta tttaagctaa gattagcttt tatacttata
     1861 atttttgaac tttcaaataa ttttgtatgt atagtaaaat actattttag tattaataat
     1921 gatataaaaa cataaagtct gtagacgaag agcagaagtt ttaagttaaa aatataaaat
     1981 atgattttta ttattatagt atttttctat aataatatct ctttccagtt agaggataat
     2041 tttcaagtga aaatacttta tggtatccaa atttctcata aaatttaggt gcttgaaaac
     2101 tgaatgtatc taaaaaaaca tatttacaac ctcgttcttt agcaattttt tcagtcttat
     2161 ataaaagttg acttccagta cctttatatc taagcttctc actaacccat aagtatttta
     2221 tagaaagcca attaccatga gtaacaccta taaaacctgc aacaatttta cctgctttat
     2281 ctcgaatata tattcctaaa tccactgggt ttttatcttc aagtctagca agattatatt
     2341 ttaataatcc ttgaaaaata gtatcttcat cttgtttttc tattttttct gtaatttcta
     2401 attccatatt tgaactttct ccttgtataa tcttttgtat attggtattt tttaataaaa
     2461 tcagtagaaa gaatatagtt taacattatt ctaataggca tttatgctaa tgtcaaacta
     2521 ataatactta ttcttaattt tatattgttg atattaaatt ttttaaaata aaaatgtagt
     2581 tt
//
LOCUS       DAEPLF010000326         2550 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.327, whole genome shotgun sequence.
ACCESSION   DAEPLF010000326 DAEPLF010000000
VERSION     DAEPLF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.327"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..725
                     /locus_tag="KR314_003521"
     CDS             <1..725
                     /locus_tag="KR314_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329873.1"
                     /translation="NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGF
                     YGVQVKKNDMKIEIHTTKEILNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAE
                     QNARQANGKSASLNGNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNT
                     NVSKIMCMINDRNSNNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRG
                     RPKKIYKVDFLSFLNKMQKLNS"
     gene            complement(976..1809)
                     /locus_tag="KR314_003522"
     CDS             complement(976..1809)
                     /locus_tag="KR314_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0329874.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENV
                     EDIKKIAYLRTLGITIDDIRNIILQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGGKITWGIITIACLLIA
                     ITVFPKLPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDILVFIVLLIIGVYKLFKKY
                     N"
     gene            complement(2138..>2550)
                     /locus_tag="KR314_003523"
     CDS             complement(2138..>2550)
                     /locus_tag="KR314_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH0329875.1"
                     /translation="GIDYENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMG
                     ASKKGSYGASIIEKIESIIPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQ
                     QLNNHEESQSHPDEQEIYEASMFAKNMMIKASIV"
BASE COUNT          942 a          377 c          275 g          956 t
ORIGIN      
        1 aaaataaatc tgcaacttgt atagttacag taaatagttc agagtacata aatgaagatt
       61 tgatatttaa aaatgatgag atacaaaatc aaatatataa cactatatta aattctctat
      121 caagacatgg attctatgga gttcaggtca aaaaaaatga tatgaaaata gagatacaca
      181 ctactaaaga aatattaaac aatatgacca ataactatac agattttttt atttcagaag
      241 atttaaaaga aattaaatat agcttaaata taggttgggg cattggaaat acaaatattc
      301 atgcagagca aaatgctcgt caggctaatg gtaaatctgc ttctttgaat ggaaactgta
      361 cttttatagt aaatgatact aatgatacaa taggcccttt atattcaaat aaaaattcta
      421 ataatattga agatagctca atagccaata aagttgcttc ttttatacct ctatctaata
      481 caaatgtatc taaaatcatg tgtatgatta atgatagaaa ttcaaataat gtgtcagcag
      541 aaatattagc agactatatg aacataactc ttagaagtgc taatagaata ttgagtatct
      601 tatataaagc aggaattgca actattgtaa atacaaaact cgataatcaa agaggaaggc
      661 caaaaaaaat atataaagtt gatttcttat cttttctaaa taagatgcag aaattaaatt
      721 cttaatattt aattttttaa taaaagcaat gtattttctc taattttaaa gaatcaaaat
      781 gtttaaattt tattagaata tacaactttt tactttaaaa tacctaaacc ttttatcagt
      841 gaaataaacc ataatatatc gtagcttatt agaaaaacaa aataatttca tatacaaata
      901 ataaaatagc cactacaatc cataatttgc agtggctact ttttacacta tcacttatta
      961 cttaattttt aaaaattaat tgtatttttt aaataattta tacacaccta ttatcagtaa
     1021 aacaataaat acaagtatat ctataaaaat aattattgta acatgtctgg caatattgta
     1081 aataaaaagt atagagaata tttctataga caacgctaca aaacacataa aattcgttaa
     1141 ataatctgca atagtatcac tataaaaagc atgtatttgt aaccatctcc atataaatgg
     1201 acgtaaaagg attctgaata caacacaaat gacagggtag gcataaataa attttttatc
     1261 tacataatta gctacaccat tattccattg gataggaatt tgaggtggca actttggaaa
     1321 tactgttatt gcaatcaaca aacaagcaat agtaataatt ccccaggtaa ttttccctcc
     1381 ccatatatat aagaaactta cagagtcaag tttaaaaaca ggtttgtttt tattccagta
     1441 ttctggcaac tcagttatgt attgctctac tttcaaattc aaataactta gctcatcaga
     1501 ttctaacatt ttattacaca tagtttttgc attttctaag tctgatattt tagtttcgag
     1561 aactttctcc tgtttctcta caacttcaac taaagatact ttctgtaata taatattacg
     1621 gatatcatca atagtaattc ctagtgtacg taaatatgca atttttttga tgtcctctac
     1681 attttcatct gaatattctc tatagccatt acgctcatta cgaaggggtt taattaaatt
     1741 ctcgttctca taaaaacgaa tattagagcg tgtcaatcct gttttttctt caacttcttt
     1801 aattgtcatt ttaaacacta cctctctatt ctattaattt acaactataa tatagtctta
     1861 aatattttca ctctataaaa ttgtatattt agtatatctt aaaatcaagt taattatctt
     1921 ttataaattt tcatacaata atatctcctt tcaataatat ctctttttct atatttaaaa
     1981 ctatcttatt ttctataaaa taatgtataa acaaggttta cagtcaagta tttttattat
     2041 ttttttgatt tcataaaaaa aagcatagca aatcatttgc tatacttttt tactaaatta
     2101 tttattctat atcttatatg aacttattgt ttaactacta cacaatacta gctttaatca
     2161 tcatattttt tgcaaacata ctggcttcat atatttcttg ttcatctgga tgactttgag
     2221 attcctcatg gttatttaat tgttggcgaa tatgttcgtt gtcaggagaa agttttaaca
     2281 tctctttata ttttgctttc aatccttctg ctatcttacc ttgacatatg aatgaaccta
     2341 atatttcatt atcttttggg ataattgatt ctatcttttc tattatacta gctccataac
     2401 ttcctttttt acttgctccc attgttccaa atagtgctat ctttttattg tgaatatttt
     2461 caagcacttg ttttgaattt ttatcacaaa ttcccttgtc cacccaatag cctaagaata
     2521 ctatatcata attttcatag tcaataccat
//
LOCUS       DAEPLF010000327         2507 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.329, whole genome shotgun sequence.
ACCESSION   DAEPLF010000327 DAEPLF010000000
VERSION     DAEPLF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.329"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     regulatory      28..124
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            178..354
                     /locus_tag="KR314_003524"
     CDS             178..354
                     /locus_tag="KR314_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329876.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(542..781)
                     /locus_tag="KR314_003525"
     CDS             complement(542..781)
                     /locus_tag="KR314_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329877.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(926..1735)
                     /locus_tag="KR314_003526"
     CDS             complement(926..1735)
                     /locus_tag="KR314_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0329878.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT          905 a          356 c          220 g         1026 t
ORIGIN      
        1 ctagtgtatt attttagttg tagaataaaa ctaaatcggc aaaactagag aaatttagtg
       61 acgcaaagct atagggacta acgcttataa aataaactat gagttatgtc agccagttgc
      121 caaaaagata ttgttctttt tgttttatga aagtttttta gggggatagt aatatatatg
      181 tttagagatg aaatggataa atgtacacac atgttaactg cttatattag tagtttatat
      241 gattattgtg attttataga tacacagcta gatgatttta tactagagta cggagaaaat
      301 gtagtagaat cttgtttaca tcaagtgatg gtattggtaa gtaagtataa ttaaatagaa
      361 attgttttct agagaaccct ttttagtttt atctacattt aaaaatgtta ttatattaaa
      421 aaactaatct cccctatatg caaagttttc ttccatataa caatttcttc ccatttatac
      481 tttttcactt gtatctttaa taaaattcaa cttcaaatat taatatactt caaaaattat
      541 tctatttctt atctttacac caattcctat cagtattatc ctttatatag gttctaacta
      601 tttcgccaca atctaaacat aaatctgaat atatagtaga ttgtactgga aaagttttat
      661 ttataacttt atctaatttt ccatctcctc tatattttaa tccaatagtt tcagcatata
      721 tgccaccagt tgttaaaata cccttttcaa tatttacact tccacaatta gaacatttca
      781 catttataac cccctaaaca attatttttt catgctatct tataatttaa aatataattt
      841 gctaccgatt ttataacaca gtagcaaatc ggtggcaaat caacattttt tcattctatt
      901 taccatctaa tccattgaaa tatggctatt tagttatagg aacttgaatt tccgttacaa
      961 actcttctac aattccagtc tcatgtatat ctatcttata gatttctata gggtcagcaa
     1021 ttatcttata attattttct tctataaatt taaacacctt ctccatatac atcttattat
     1081 tagaattact cccagtataa tttaaagtta cataatatcc ttcatcaaaa actatattat
     1141 atactttctc attactttcc agaaaacaaa atacagattt aaactcatta aatatacctt
     1201 tattaattgc ttcagtagaa aaggcagaac caatattgtt gtttccaagt atattaaatc
     1261 tatcttcata ttctttctga agtttctgaa ttaaaaaatc gaagtcttca tccctcttta
     1321 tatcagcatt taattctaaa gcttttcttt tttctatata tacaacctct atctcatcta
     1381 tttttgtatt ttgaattaca ctttcaatcg aactcaatct ttttataata ttttccttat
     1441 ggcttataaa ttcagctatt tttttatcaa ttaaatcgag ttcttcattt aaaatatttt
     1501 tcgtagactc tatacttctg tcatctaggt attcttttat tttcttcatt ggaaagttca
     1561 agcttctaaa ttcttttatt aaatttaatt tccatatatc tgatatatta tacattctat
     1621 atccatttat atctctaact ggtcttagaa tgcctatttc ttcataatac attaatgaat
     1681 ctctacctat tccatatatt ttagatatct ctcctatttt atagtaatct ttcatttata
     1741 taataaactc ctccgaaatc ctatttttat tttaaatcaa cattattact ctataatact
     1801 ataatattag aatatactaa aaaaatcttt aaaacaagga taggaaaata aacatcctaa
     1861 taattaatta ttaaaatatt tttatattgt aatgttaggt atagtattta taacttcaac
     1921 ttttttataa atatatttta gttttatata gttttatgtt tacagctatt atatccatct
     1981 ctatgtaata tttttcatat taaaactgat ttctatagat taatagttta taaatttagt
     2041 aaatgaatat ttattatagt ctttaacaaa aaatacaaga ataaacttaa ttttaataac
     2101 taaaattaat tatgtggtat taaattatgt ataaaaagtt aattatgaat aaaataatta
     2161 tgcctttgaa aaatcattaa acttaaaatt aattataaaa aaactctacc ttactaacta
     2221 tacagctcac aatctatcta tttatgaaaa aatatgatat aattgcattc aaaaaggaaa
     2281 tctttcaata tactataatt taagcattaa ctaattttat ctaatattat tactaaacta
     2341 aaaaatattt gtatttgtag cattggagca aataacttaa tgtatattca ttttaaatac
     2401 ttatttaaaa tttagcattt aaattattta caaattaaca tttaaatact tctagcataa
     2461 atacagttgt catcaaaatt taaagtatct tatggaggaa taccatg
//
LOCUS       DAEPLF010000328         2472 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.330, whole genome shotgun sequence.
ACCESSION   DAEPLF010000328 DAEPLF010000000
VERSION     DAEPLF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.330"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..1151
                     /locus_tag="KR314_003527"
     CDS             <1..1151
                     /locus_tag="KR314_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBH0329879.1"
                     /translation="NMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIK
                     GFYKNLVEEKDAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVN
                     PIYSNGKEIFNLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKEL
                     NRELNKTKKSDLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIIDIDNIDDSFYL
                     SYISRCVYFNSKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEI
                     FTIIIIAISNLMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPI
                     GIISTRVDLSNNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            1156..1773
                     /locus_tag="KR314_003528"
     CDS             1156..1773
                     /locus_tag="KR314_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329880.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKNLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            1962..2306
                     /locus_tag="KR314_003529"
     CDS             1962..2306
                     /locus_tag="KR314_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBH0329881.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
BASE COUNT         1071 a          239 c          370 g          792 t
ORIGIN      
        1 ataatatgct acaaactcag ttaggaaatc tatctgagtg ggataaagca aaaaatgttt
       61 ataatcttac tcttaaagat acaggcgaac aatctttagg aaaggaagaa gtaattaaaa
      121 atgccaagat taaaggtttc tataaaaatt tggtagaaga aaaagatgct ttcttaatag
      181 attcaactaa ttttgaaaaa cttgaagatg gaagttacat gtatgaaata aattcaaagg
      241 gtaaaaaccc agaagtaagt caatatggta aaaatataaa aataaataaa aactatctta
      301 aagttaaccc tatttactca aatggcaaag aaatatttaa tctaatcgat tatgacgata
      361 agactataaa tttattagtt cctaaaaagt tacaagtaca tgaaaatgag attaaaaaag
      421 agtttagaga attattttat tttgaaaaaa tagaagtaga aaatatgtac aataaagagt
      481 taaatagaga gttaaataaa acaaagaagt cagatttaaa tgttaatata atatatgtag
      541 ataataatca aagctatttt acttataact cccttgttat ggatgacaat aggaatctca
      601 ttgatgaccc aatagcaata attgacatag ataatattga tgattctttt tatctttctt
      661 atatatctag atgtgtatat tttaattcta agaaattaga tgcccttgca gatataagta
      721 acattattga agctcaaggt gtagaagcgc atatccaaag tttacatgct gtttataatg
      781 agtatggttt agaaataaat aaactagaaa aatatttgaa tagtgaaatt tttactataa
      841 taataatagc aatatcaaac ttaatgatta cctacaatat agtagctagc tactatgaaa
      901 gaaataaata taagttatat attaaaaaaa tatttggtta ttcaactaca gctagaagta
      961 tgttgttaat agtctcatta atattaacaa acattattcc aataggcatt atatcaacaa
     1021 gagtagattt aagtaataat attatcttat ttggacttct gatattagtt atagaagtaa
     1081 ttgtatctat tgtattagac aagattatct caaatagttc atttaataaa attataaaag
     1141 gagaacatta aaaagatgat ttcattaata aatatatcta aaaaatttaa agataaatct
     1201 ataatagaca aatttaacct agaaatttct aagggcgagt ttgtagcgat aacaggaaat
     1261 agtggttcag gtaagacaac tttacttaat ataatgggat tattagaaaa acctaattct
     1321 ggtgatatta taataaataa tataaaaaat ccaaatagta aacagataag aattttacaa
     1381 agggattttt atggctatct atttcaaaat tatgctttaa ttgagaatga aaatgttgaa
     1441 aataacttga aaattgcttt aaagtatcaa aaaaatatca accataatga aaaaataaat
     1501 tacgcacttg agtctgtagg tcttagaaaa tacaataaaa aaaagatata cgaattgagt
     1561 ggaggagaac aacaaagggt agcattagca agagtaatat taaaagaatg tgaagtaata
     1621 tttgcagatg aacctacagg taatttagat aaaaaaaata gagacatagt atttgatata
     1681 cttaaaaatc taaataagaa tggtaaaact atcgtatttg taactcatga tttagagtta
     1741 tcggagcagg cagatagagt tataaaattg taataatatc aactataata aatataagaa
     1801 agagattaca gctaaatcaa gtttaataat aatacgaata gaatttaaac attaagtaaa
     1861 ttagtagttt gtaaatataa gtataatatg atatcataaa ggctcaaaag aaattttata
     1921 taataagaaa tttaaaaata acaaatggag gacatataaa gatggaaaat ttaccaaatt
     1981 gcccgaaatg taattcagaa tatacttatg aagatggaac tcttttagtt tgtccagaat
     2041 gctctcatga gtgggcatta ggattgggag ctgaagaaga ttcaaatatt atcaaagatg
     2101 taaatggaaa tgtattaaat gatggagatt ctgttacagt aattagagac cttaaagtaa
     2161 aaggaagttc atcatctata aagattggaa caaaagtgaa aaatatccgt ttaatccatg
     2221 actcatcaga tggacatgat atagaatgta agatagatgg ttttggagct atgaagctaa
     2281 agtcttctgt tgttaaaaaa gcgtaatatt ataaataagc tgtagtgttt attgtttttc
     2341 agtacagtat aaaaccataa gaatctatta agtaataaaa gtttgtttat ttatctttat
     2401 attttcttat ggttttatta aatataaatt ttattgaaca ccaaaatatt ctctcctcta
     2461 caaataagcc aa
//
LOCUS       DAEPLF010000329         2471 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.331, whole genome shotgun sequence.
ACCESSION   DAEPLF010000329 DAEPLF010000000
VERSION     DAEPLF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.331"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(96..965)
                     /locus_tag="KR314_003530"
                     /pseudo
     CDS             complement(96..965)
                     /locus_tag="KR314_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
     gene            complement(1258..1728)
                     /locus_tag="KR314_003531"
     CDS             complement(1258..1728)
                     /locus_tag="KR314_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBH0329882.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
BASE COUNT          810 a          400 c          191 g         1070 t
ORIGIN      
        1 tagatttttt caaaacactg gataaatagg taaaaaaata aacagttagt aaaataaatt
       61 tttactaact gtttattttt attgtttaat atgtcctata aatcctctaa gtaatatttt
      121 ccttgcaaaa actctgattc taaacctaga tttttaaaat aatcaaccat gcccatacac
      181 gcaaattttt ctgtagaata atgagtagca cctataacat taattaaatt atcttttgct
      241 atttgatgga actctaatac tggttcaaaa tgttttaaag gcttagtaaa cccagtaata
      301 tatagattta taccaagttc agcaacttct cttgctacaa atggataact tcctccccct
      361 gctgcaatag caactaaacc atcttttaat acagaatctc cataatcata caatttcaca
      421 tcatgaccaa cagatttttt aattaattcc ttaatctctt ctattgattt tagtgatgtt
      481 ttacatataa ctcctacctc aattttatcg tcatatttac aaaatggttt gactatttct
      541 aaacccatag cttttgcaaa actaacagaa gttgaatagt cactatagtt gtctagtgga
      601 gaatgtaata cataaaaaga aattctacga tttttcatct cttccatata agatagtggt
      661 atgtcgtgaa atggaaaacc ttcatctgat gctatgtatc ccattgcgtg atgtgaaaaa
      721 attaaaatat ctttttcatc tttatctata acttgcttaa ttatttcttt atcaggaaaa
      781 gtagttgtgt atactttatt aatattttga gcattatcta atactaatca tatgtacttt
      841 tttctaaatt ctggagtgat aaaatcattt tcaaaattca taaaactcca atcatcatta
      901 atatttttaa tattaaattc taaatctaat tttttataaa gttcaattgc cttaataata
      961 accactcctt tataataata ttgaattact acatattaat atagaaattt ccgataataa
     1021 actttatacg tttaagtatt cctacttcaa attaaaaata ttataaatat tcaaatttat
     1081 taatatattt taatattata tttatttttt ctttattaat attcccctta tttttcttta
     1141 ttaatatttt ctttatttta atttaattta tgacatttgt caaatactag tcctatatat
     1201 tatcttgaat tagttccact acaattaatt tctactaaat aattataatt taaacaatca
     1261 ttccttacta ttttcacact gctttacaaa ttcaattgga tgttgaatta ttttagaagt
     1321 ttcttctaca gagtatccct cagataaata tcttctagct ataacttcca tagactctcc
     1381 tatctcaata atatgcttat ctgggtcata aatacgcact actctttgtt gccattcatg
     1441 ttttagtggc ttatggacaa actctacatc tttataataa ttgagttttt caataaattc
     1501 gtcaaaatca tcaacctcaa aataaagttc catattattt gatttttgta taattgtatc
     1561 tggattaatc cctgtaagcc atgcgaagtc ttcttgtata gcaaatcctc ctttaaatgt
     1621 tacatttttg cctaagtcca attcaatttc ttgctcaaat aattctttgt aaaatttttt
     1681 tgatacttca atatccttca ctgctaataa tgaaacctga tatttcataa attttcctcc
     1741 taatttttta tttctatttt ttcagtatct tctattaaca tttttttctc tatctcttca
     1801 gtacctttta ttagtccttt atttttactt ttttagtatc ttctattaac acttttgttt
     1861 ctacctcttc agtatattat atcaatcttt tatttttatt tttcaatatc ttctattaac
     1921 acttttatct ctatctcttc agtacctttt attaatcttt tgtttttatt ttttcaatat
     1981 cttctattaa cacttttgtt tctacctctt cagtatcttc tattaatcct ttatttttac
     2041 tttttcaata tcttctatta acacttttat ctctatctct ttaatatctt ctattaattc
     2101 tttattttta ttttttaaat atcttcatta attcctattt attttatcat cttaaatacg
     2161 aaaacactgt gtcatatttg caaaatatat attgtcatct atattaaagc ttcacaattt
     2221 atatttaaaa attctcttta atcacactac ctctatttaa aaagctacct taattatact
     2281 tataagtaaa ggtagctttt taaatttaat taaatagaat atccactatc aatttatatt
     2341 taatttattt agattcacat tatagtatca taatccactt ataatcattt gtcatattac
     2401 ttaagtagac taatatatta cataaataca cttacataca ctttatatta atgctataag
     2461 aaatacgttt g
//
LOCUS       DAEPLF010000330         2418 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.332, whole genome shotgun sequence.
ACCESSION   DAEPLF010000330 DAEPLF010000000
VERSION     DAEPLF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.332"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            164..748
                     /locus_tag="KR314_003532"
     CDS             164..748
                     /locus_tag="KR314_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBH0329883.1"
                     /translation="MKKFFMVVLGCIFVLGVISGCSKKESKPVEKNKSNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKNIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEEWKKIIDERLKMVKANE"
     gene            741..890
                     /locus_tag="KR314_003533"
     CDS             741..890
                     /locus_tag="KR314_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329884.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            901..1800
                     /locus_tag="KR314_003534"
     CDS             901..1800
                     /locus_tag="KR314_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0329885.1"
                     /translation="MKLLSKVNNNIRLIIQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            1805..2374
                     /locus_tag="KR314_003535"
     CDS             1805..2374
                     /locus_tag="KR314_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329886.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
BASE COUNT          933 a          222 c          446 g          817 t
ORIGIN      
        1 aagattttgt ctgaaaagaa atagatttta actatatatc ttttatacaa cttattttaa
       61 attagttctt atttattaaa tataatgttt taatatagaa gagaagttat ttttatttca
      121 agcagccatt ttatatacta tataaattct aggagtgtga gatatgaaaa agttttttat
      181 ggtagtttta ggatgtattt ttgttttagg tgtgatttct ggatgtagca aaaaagaatc
      241 taagccagta gaaaaaaaca agagtaatag tgaatggttt agtaatttag atacggagga
      301 tgttgatgga aataaggtaa caaaagagat attttcaaat aaagaactta ctcttattaa
      361 tgtctggact acttggtgtg gaccatgtgt gggtgaaatg ccagagcttg aagcattatc
      421 aaaagaatat gagagtaata attccaatgt agctataaag ggattagttg tagaagttga
      481 taaaactgat atgagaactg gtctttcaaa tgaagagaaa aatttagtta aaaacattat
      541 gaaaaagtca gatgcaactt accaacaatt gactgtatct gaagaactta aaaaaactga
      601 ttttaaaaga gttatggagt ttccaacaac ttattttgta gacaagaaag gaaactttgt
      661 gggtgagaag gttgtaggtg caaatagtaa ggaagagtgg aaaaaaatta tagatgaaag
      721 actaaaaatg gtaaaagcca atgagtaact ttattaaaga taataaaaaa ggcatttcta
      781 ttattgcaat tagtattata tttattgcat taggtacata tcgtggagaa acaaatacag
      841 tattaactaa agcaattatg atttgtcttg agtgtattgg tattggttag gagaagagga
      901 atgaagttat tatctaaggt aaataataat ataagactta ttattcaagt tgcatttaca
      961 gcattaacaa atggatatgt aaatggattt ttagaaggaa ctatatatgg aggagaaagt
     1021 aaaaaattat gtgtacctgg attgaattgt tattcatgtc caggtgcact tggagcatgt
     1081 ccaataggtt cattacaagc agttttatca agcagagagt ataaattctc attttatgtt
     1141 gttggttttt taatggcatt tggtgctttt tttggcagat ttgtttgtgg ttggctttgt
     1201 ccctttggac ttgtacaaga tttactacat aaagtaccct ttccagtaaa gataaaaaaa
     1261 gtttatggtg ataaatatct aaaatatcta aagtatatag tcttaataat gtttgtaatt
     1321 attttgccag ttgctattgt aaatgtagca gggggtggaa atccttggtt ttgtaaatgg
     1381 atttgccctt caggaacact tttgggagga ataccactta ttttaggaaa tgagaattta
     1441 agagaatcaa taggattttt atttagctgg aagttaagta ttttgctatt gatattattg
     1501 atgtcaatca ttacttatag gccattttgc aagtatatat gccctttagg agcaatctat
     1561 ggttttttta atcctatttc aatatataga tttaaaattg ataaagataa atgtacaaat
     1621 tgtacagcat gtcaaacaaa atgtaagctt gatataccaa tatataaaga tgtaaatagt
     1681 ccagactgta ttaggtgtgg tgaatgtata aaagtttgtc cacaaaatgc aattactaca
     1741 actttttcaa aagataaaaa agagaataat atttgtgcaa aaaagtatag ggggatataa
     1801 tttaatgaag aaatttattc tatgtattat aatgtttgca atatcagtac tggtgttagc
     1861 tggttgtact aatgataata agataaaaaa tgaagtggat agtatgttta attcaataaa
     1921 aacacaagat gcagatactg ttgataagta tttcccaaaa tcagggttag gtaatcttat
     1981 atacgaagat aaagaaggat ttaaaatgta ttctaaaaat atgacttggg aaatttcaga
     2041 tataaagaat aataaggata aagtgactgt agatttaaaa attaacaata ctgatatggt
     2101 ttctatttta aaaaagaatc ctcctaaact aggagaaatg atgccaaatc caactatgaa
     2161 agatattgat tctgcaaaga aaaaagaatt taatattaca ttagagcttg ttaaatctgg
     2221 tgataattat gtttgtaaag ttaatcaaga atctgcaatg aaattattaa atgtgattac
     2281 aggaggtggt atagattatc tagctgacta taataaagat tattataaac agcttgatga
     2341 agaacaaaaa aattatgaaa ataaaaatag ttagtaatat atatgaaaat tggagcaatt
     2401 aataatatag aaacacaa
//
LOCUS       DAEPLF010000331         2347 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.333, whole genome shotgun sequence.
ACCESSION   DAEPLF010000331 DAEPLF010000000
VERSION     DAEPLF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.333"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            170..1516
                     /locus_tag="KR314_003536"
     CDS             170..1516
                     /locus_tag="KR314_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0329887.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGIQQMLFSIGNMTLQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGYWKTKAIVNHRINE"
     gene            1594..>2347
                     /locus_tag="KR314_003537"
     CDS             1594..>2347
                     /locus_tag="KR314_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBH0329888.1"
                     /translation="MGIILKPKQFYMTVIVTIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSY"
BASE COUNT          764 a          296 c          355 g          932 t
ORIGIN      
        1 tattcgatta tattaaatat tgatttatta aattcaaatt ataattatat tctttacatt
       61 ttaaaaaaat atatgatatt ttgatacaat ttatttatag tttggatata aagtttatgt
      121 taagattata ctataataaa ttaataattt ttttatttgg aggaataagg tgaaagattt
      181 aactacaggt catgagggaa aatccatatt cttttttgcc atgcctatgc ttattggtag
      241 tttatttcaa caactataca atacagctga cagtataata gttggtagat ttatcggaaa
      301 agaagcaatg gcggcagtct ctggagctaa ccctataatg tttttattgg tggcagcgct
      361 tatgggtgtt agccttggat tttctatttt agtgtcacaa ttttatggct caggagattt
      421 aaaaaaggta aaagctacta ttgatactac atatatactt ttatttattg gttctatatt
      481 aataagtatt cttggaatag tatttggtgg cccaatgctt aagcttatga atacaccaga
      541 atccgttttt gctcaatcaa aattgtatct tactataata tttagtggaa tattattttc
      601 tgctggatat aattcagtaa gtgcaatact tagaggactt ggagattcag taactccact
      661 ttattttctg ataatagcca caattctaaa tatagtttta gacttaactt ttattgtagt
      721 gctaagaatg ggtgttgagg gcgttgcact tgccactata atggctcagg cagtatcatt
      781 tataataagt ataatttatt taaataaaaa acatgaagtg cttaaattta aaatcaaagg
      841 aattgtatac gataacaaaa tatttaaaga tggacttaga ttaggacttc caagtggaat
      901 tcaacaaatg ttgttttcaa ttggaaatat gactcttcaa tttttagtta atagttatgg
      961 tacatctgct atggcagctt ttggtgctgg tcttaggata gaaaatttta taagcttacc
     1021 tattatgaat cttggttctg ctgtttctac ttttgttgct caaaacatag gtgctggaga
     1081 aaatgaaagg gttaaaaaag gcattcgaga atctataaaa atgaccttag ttttagcagt
     1141 tacagttatt gctttaattt tgctatttag ggaaaatcta attgctctat tcaatactga
     1201 taaagatgtc attaaaattg gctcttctta cttatttata ataggaccat tctttttatt
     1261 tattggaact tcttttgtac tttcaagtgc aatgaaaggt gctggtgatt ccatgtttgc
     1321 acttataagc tcaatagtat ctttatggct tggaagactt ccagcttctt atatgctttc
     1381 taaatttttt ggaacagacg gaatttggat gggtattcct tttggttgga ctttaggtct
     1441 tattgttaca gttatctact ataagaaagg gtattggaaa actaaagcta ttgtaaatca
     1501 tagaatcaat gaataatctt tttataaaat gtattcacat tcattgtatt cacctccatt
     1561 atgatataat aaattatgga ggtgaatata aaaatgggta ttattcttaa gccaaaacaa
     1621 ttttatatga ctgttatagt taccataatt ctctacttct ctcttaatta cagtttcaac
     1681 ataaaatttt ctatatgctt atctttttta actatcttga tatgggctgt agactcagtt
     1741 gaaaaaactt ttgttgcatt gttttttgta attctagcgt ttatatttaa cattgcacca
     1801 ataaaggtta ttttacagtt tttatttaca gaaaattttt acataataat tcttgcatac
     1861 ataattacta atgcagttac aaaaacaggt gtcgcaaaaa ttatttctga gaagttaatt
     1921 ttaaatactg taaatacccc taaaaagatg attcttttat cgtatatttt aggtattctt
     1981 ttaatatttt ttatcccaca accatttcca agggttattc ttgtatctgc tttttacaag
     2041 gagtttttaa aagaacaaca gataactgaa gattcaaaat ctatactttt atttagtata
     2101 tttacagcat ctacctttac atcaatgttt tttgtaaatg gagacaccct tctaaattat
     2161 gttgtactag aacttggaaa ttgtaatata aattggggtc aatgggcttt ttatatgtct
     2221 gttccaacta taattacttg ttttataact tattttttat ttatctttgt ctttaaaaaa
     2281 gaattgcatt caataaaatt tatttctaat gaaaatcttg agggtaataa acttcatttt
     2341 tcatatt
//
LOCUS       DAEPLF010000332         2291 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.334, whole genome shotgun sequence.
ACCESSION   DAEPLF010000332 DAEPLF010000000
VERSION     DAEPLF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.334"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            47..2275
                     /locus_tag="KR314_003538"
     CDS             47..2275
                     /locus_tag="KR314_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBH0329889.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT          860 a          300 c          359 g          772 t
ORIGIN      
        1 attgattata aatctaactt ttttctttat caaaatattt taggaggtga gacatttgaa
       61 aaaaactaat agtttaaaag aaattttaaa ctggaataat atcaagaatt taagaccttc
      121 aaagggttta aaaaatatat tagaaatttt taaaagtgac ctaaaagata tacgtggcaa
      181 ttatgccgta cttgtaatag ttttaggtct tgctttttta ccatctctgt atgcttggtt
      241 caatataaag gcatcttggg acccttatgg aagcactgct aatatgcttg tagcagtaac
      301 aaatgaagat aaaggaaatg aaatagcagg acaaaagatt aatgtaggag accaattaat
      361 tgaacaactc aaagataaca agagtttagg ttggcagttt gtagatgaaa aaactgctct
      421 tgaaggtgtt aaaaaaggta cttattatgc atcacttaag attccaaaaa attttacaaa
      481 agatttgaca tctttaatta cagatgatgt taaaaaaggc gaaattatct atactgttaa
      541 tgaaaaaata aatgcagttg caccaaagat tacagataaa ggtgcttcta caatacaact
      601 acaggttaat cagactatag taaaaacagt tagtgaaatc gttcttggta tatcaaacgg
      661 agttggtgca ggaattgaac acagtcttcc aaaattgaca aaagttgaaa attcccttat
      721 tgaccttcaa aagagatttg accatataaa taagactgtg gatttagctt ctgatgctac
      781 ttcaaaatta tctgatacag ttaaagatat taaatctgat ttacctacaa ttaaaaagac
      841 tctgaatgat acaaaactat tatcaagtga cttaaaaaaa ttcttagagg atacaaatga
      901 taatttagac gaactttctc ctcttattaa gtctgactta aatttaatgg ttgacctttc
      961 atcttctgct tcttctctta cattaaattt aattgatgca gtaaatagtg gcagtgaaga
     1021 tgtacctaag ttgatagata atttatcaga aaaactttct aatttgcaat ctttaaacga
     1081 tactttggtg gactttttaa ctaaattaaa ccaacttaca tcaaataata gattggatga
     1141 tgtaatagat aatcttgaag actctagcaa taaaatagat tcatctatat caacattaaa
     1201 tgatattaaa aacaaagtta tatctggaca acaaccatct ataagtgctt tgaataatgt
     1261 tttaagtcta tcaaatggta ttggaagaat taatttaaat attttaaata attttgactc
     1321 aaaaatatca aaacctataa atagtatatt tgcaaacagt ataaaagtag caaatgatat
     1381 aattactgtt ttagataagg cagaagcaaa acttccaaag gttgaagaaa tacttactac
     1441 ttctctaaaa ctttcaggaa atgcacagga aagtatatca ttgataagag aaagacttcc
     1501 tttagcaaaa ggaatgttgg atgatttgat tgatacttta agcaaaatta gtaatggtga
     1561 agatatgaaa aaattagtca gcttgcttga aagtgatata ttgacaaagt ctaatttctt
     1621 aaaagaacct gttgaggtga aaacagagaa attatatcca attgaaaatt atggttctgc
     1681 aatgacacca ttttatagtg ttctttctac atgggttggt atattactta tttcagcact
     1741 attgtctaca tctgtacatg gaaactataa accttatgaa atttactttg gaagaggtct
     1801 tactttttta agtatagctc ttgtccaagg ttttataata ggaactggag atatattatt
     1861 gttaggagta acagcaaaac atccaatttt attgatagtt ctgcttatgt ttactagtat
     1921 ggtatttaac tttatagtgt attctttagt ttctgtattt ggaaatgtag gaaaagcaat
     1981 tgcaatagta ctattagtcg ttcagattgg ttcttctggt ggtacattcc ctatacaagt
     2041 tacacctaca ttcttccaaa gaataaaccc tataatgcca tttacctatg caataggtgc
     2101 tacaagggaa gcagttggtg gtatatatgc tccaaatcta actaaagata tactagttct
     2161 attactattt atgattctgt ctatttgttt aaacgtactt ctaaaaggac ctataaatag
     2221 agttataaaa ccatttaatg ataaatttgg cgatagtaat ttaactggtc attaaaaaat
     2281 taattctgtc t
//
LOCUS       DAEPLF010000333         2290 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.335, whole genome shotgun sequence.
ACCESSION   DAEPLF010000333 DAEPLF010000000
VERSION     DAEPLF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.335"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(319..1098)
                     /locus_tag="KR314_003539"
     CDS             complement(319..1098)
                     /locus_tag="KR314_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBH0329890.1"
                     /translation="MNSKIYYFSGTGNSLVVSKILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIIKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPSDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(1166..1627)
                     /locus_tag="KR314_003540"
     CDS             complement(1166..1627)
                     /locus_tag="KR314_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0329891.1"
                     /translation="MGGAGDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(1603..2280)
                     /locus_tag="KR314_003541"
     CDS             complement(1603..2280)
                     /locus_tag="KR314_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0329892.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
BASE COUNT          764 a          408 c          216 g          902 t
ORIGIN      
        1 attgaaaaat caaaatttga aatggcatca gaagatataa aaaaaatgtt cgagattata
       61 aacggttgat attacaaaat aataaaaact aatttagcca aataataagt gcctcaaaaa
      121 cttctattta tgaggcactt ttattttata ttagtgtaac tatatactat taacataatt
      181 tattatttat attgttcttt ttaatctcta ttccatttag tctaggtaac attactcttt
      241 acaagattac tttatatttc ttttatatct ctaacgtttt tatttaataa aacaattatt
      301 aatacaagtc aaatatatct atttatctaa aaatatatct tttacagtaa tatctggatg
      361 gtgatacttc ttcctattcg ttaaaaaaga tttttttaca ttaatagcct cttttggaca
      421 ccagtgaaaa caagctaggc atctttcaca ctcattaccc cactttggct ttttatcaac
      481 tatacttata ttctctacag gacaaaccct tttacatgtc ccacatccat tacatttatt
      541 ttctgcaaaa aatttagtag ttgcaatttg catattctta cctactaaac gaagtacaac
      601 actatctata tgtgacttta aattgttttt tccttctata tttccaatct ccaagttatt
      661 gatacatttt gcaatctcag ctaccctatt agtctgattt ttcaatcttt cttcttcaac
      721 atctgaaggt gttatcaaag catttccagg catattgatt aaaaaaccag cagataattt
      781 tttccccctt tttttaagta acttatctat agtaaataaa ctatgtccag ctaccccatt
      841 acaagtggca atggcatata tatgagtatt aatattaaaa tttaatttct ctacaaaaga
      901 ctttattata tcaggaacat cagcaaaata aacaggaaaa acaattacca atatatcttc
      961 atttatgtct atattatttt cttttctatg tatagctaaa ggtataactt ctttacttcc
     1021 atttaacgtt tcttttaaaa tctttgaaac tactagcgaa tttcctgttc cagaaaaata
     1081 ataaatctta ctattcattc ttatccctct ttctatattt ctacatcatc aatagttaaa
     1141 aagtctctta aaataggatt tatatttact ttccaattct atccaaaata tcagtaaaca
     1201 aaggcaattc ttcatcagag atagatttaa atgtctctgt tgcatatata cttgcaattt
     1261 catttaatgg ttcttctaat tctctaccta aatctgttag gaatatttga aacttacgtc
     1321 tatctttaac atctacttta cggacaatta atcccttatt tttcataacg tctaaaattc
     1381 ttgttgtatt agatttatcc ttacatgatt tttcacataa atctatctga gaaattccat
     1441 cttgctccca tagaaacctt aataacgcat aatggtcagt tgttatatca tatccatttt
     1501 gtttaaacat cttttttaaa tcatctttta cttttaacga agtacttact aacttagttg
     1561 tagcttcata tagtttctca aaatctttca ttttacctcc atttaaataa tcccctgctc
     1621 ctcccatcta tcaattattc ccttatattg ctcaatatct ttaaaagcca ttggctttaa
     1681 aacagcattt atagcaataa aattatagat ttgagaaagc acaggtggat ttttcttttc
     1741 ctctatgcaa taaataaatt ttttagatac cttttgtatc tttgattcta ctttttcagt
     1801 tataggattt tgattaaatc cagtttttat agataaacta ccacaaacat aacctcccga
     1861 atctaagacc atggatttta taaaagaaat tgcttttgaa ccacgtgcat tttcatatgt
     1921 agtaactgct atacatggct tacgatacaa taatctttca agcatcatat gtgctctatc
     1981 tgtaaacacc ttgaaaagtc cagttacact acttccataa gttgggctac caaatataac
     2041 accatcactc ttagtaatta tatcatgaat atactctact ttatcatttt gattgataca
     2101 cttgcccatt ttataacaat ttaaacaacc aatacaatat gatggattta cctcacttaa
     2161 atcaaaataa ttaattttta catctggtat taatctttct atatctttat acaagtaagt
     2221 taaaactttt gatgtagctc cattttttct agggcttcca tttataactg taattatcat
     2281 ttctatatct
//
LOCUS       DAEPLF010000334         2277 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.336, whole genome shotgun sequence.
ACCESSION   DAEPLF010000334 DAEPLF010000000
VERSION     DAEPLF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.336"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..1964
                     /locus_tag="KR314_003542"
     CDS             <1..1964
                     /locus_tag="KR314_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0329893.1"
                     /translation="FLENNLKLVFGDSININRYFINEINDNDIINDDVILVMSVERLD
                     KIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNALIVNDSDETTLETISLFYKIGV
                     TNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGHRYIDISTFIEIINLLQI
                     DSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTILNLSKDGILFTSKDGEIN
                     TYNSKVKDILDINEDIYGKYIDDIFVDSLKVLLSEKEILDKVVVFNKKYINVNKKNIY
                     NRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFKDIKTNSPKMFECIDLAK
                     KVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVNCAAVPENLLESQLFGYD
                     KGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLLRVLQEKQIMPVGSHNVI
                     NIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPLRERKEDILIIMEDLINK
                     KLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLEKDLPPNLRSSDNKNTSL
                     KLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQITEGKIKKIFEYLKKEE
                     LIICSSGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT         1013 a          226 c          313 g          725 t
ORIGIN      
        1 actttttaga aaataatttg aaattagttt ttggagattc tataaatatt aatagatact
       61 tcataaatga aataaatgat aatgatatta taaatgatga tgtgatatta gtaatgtcag
      121 tagaaagatt agacaaaatt ataaataata tactagataa gaaaaaagtg attgtggtaa
      181 gaagaacttt tagggaagat aaaatttata atctactatc tttaccacag ggtacaaatg
      241 ctctaatcgt aaatgattct gatgaaacta ctctagaaac tataagttta ttttataaaa
      301 ttggagtgac taatattaga ccaattccat atatgaatga taataactat aaaaatataa
      361 aaatagctat aactccagga gttccagaaa aagtacctag ctttatctca gatatatttg
      421 atttaggtca cagatatata gatatttcta catttataga aataataaat ttattacaaa
      481 ttgattcaaa ggaaatacaa tctaatctag ttaaatactc agaagaaata ataagtttag
      541 atactggtat aaaagataaa tataaggaat tatttttaaa aatagaagaa ttagatacaa
      601 tattgaattt atcaaaagat ggtatattat ttacttcaaa agatggtgaa ataaatacat
      661 ataatagtaa agtaaaagat attttagaca taaatgagga tatatatgga aaatacattg
      721 atgatatatt tgtagatagt ttaaaagttt tattgagtga gaaagagata ctagataaag
      781 ttgtcgtatt caataagaaa tatataaatg taaataaaaa aaacatatac aatagggatg
      841 aaaaaatggg cacatattat agtttacaag aaataacata cataaaaaaa ttagaacaaa
      901 atctcactaa aaaattaaga gaaaaaggac aaatagcaaa atatacattt aaggatataa
      961 aaacaaatag tcctaaaatg tttgaatgta tagatttagc taagaaggtt tctaaatctg
     1021 acttaagcat acttatcaga ggtgaaagtg gaactggtaa agaactcata gctcagtcta
     1081 tacataacaa ctcaaataga aaaaatcaac cttttatagc agtaaattgt gcagccgttc
     1141 ctgaaaattt attagaaagc cagctattcg gatatgacaa gggaactttt actggaggtc
     1201 taaaagatgg aaaacaagga ttatttgaac ttgctaataa tggaacaata ttcttagatg
     1261 aaataggcga tatgccttta gaattacaaa caaaactact tagagttctt caagagaagc
     1321 agataatgcc tgttgggtct cacaatgtaa ttaatataga tgttagaatc atatctgcta
     1381 caaataagaa tttagaacaa atgattgata atagccaatt tagagaggat ttatattatc
     1441 gattaaatac aattccaata aatatacctc ctctaagaga aagaaaagaa gacatattaa
     1501 ttattatgga agatttaatt aataagaaat tagtaatcac accagaagca aaaaaactta
     1561 tacaaaacta tatgtggaaa ggtaatataa gagaactcca aaatgttaca tcctatttaa
     1621 acattatgtg tgaagatatt gtattagaga aagatttacc tccaaactta aggtcttcag
     1681 ataacaaaaa tacatcatta aaactaaaat atagtaagaa tgatatattg aatatattag
     1741 agatactaat tttaaataag gaatcagatg ttggtattgg taggggatta attttaaaag
     1801 cactactcga taagaacctt caaattacag aaggaaaaat taaaaaaata tttgaatatc
     1861 taaaaaaaga agaacttata atttgtagtt caggaagata tggaagtaaa ataactcaaa
     1921 aaggagaaga tttttacaat aaattaaaat ataaaggtct ataaaattat aatttatcaa
     1981 acaaagaaat ctattcagga ttaaagtact ccttcaatag gtttttttat ttttcaagta
     2041 ccctaaaatt ataatttatt ttgtacataa ataataggaa ttaataggaa taggataatt
     2101 tttttatttc ctattttttt tatataaact agcatataaa atatattttc ttcaaaataa
     2161 tattttttac tgaaaattga aactatgcaa tcatttttta tactatacat atagataaat
     2221 gattatattt attcaatatt cttataaaaa acaaaattct ccatcgaaaa ttatggc
//
LOCUS       DAEPLF010000335         2240 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.337, whole genome shotgun sequence.
ACCESSION   DAEPLF010000335 DAEPLF010000000
VERSION     DAEPLF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.337"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            328..597
                     /locus_tag="KR314_003543"
     CDS             328..597
                     /locus_tag="KR314_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBH0329894.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            623..1978
                     /locus_tag="KR314_003544"
     CDS             623..1978
                     /locus_tag="KR314_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBH0329895.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPISTNSSELFTQRGGRIPAPIHSFK
                     N"
BASE COUNT          868 a          223 c          449 g          700 t
ORIGIN      
        1 gaaaaagtat aattaaattc ataaaattaa aaaaataatt tttagagagg tattatttag
       61 aaaatcacga gattgctaag taataccttt cattattatg aaaactgtat ttattgttga
      121 gaaaacatat ataaaatatg aattttctaa atagtataaa aataatggaa tattgaaaaa
      181 aaattgacaa atgaatgtat aggtattaaa atatattaaa tatatacata aaaagtggtt
      241 tactaacaaa taaatgaaat atatgttaat acatagttca atttgtaaaa atataagatt
      301 tataaaaaaa taagggagga tattattatg aaagcaattt taacagtaat tggaaaggat
      361 aaaccaggta tagtggcagg aattagtgat gaattataca agcaaaatat caacatagta
      421 gatatatcac aaaaaatatt acaggattat tttactatga ttatgatagt tgatttagag
      481 aaatcattaa attcttttga gaaaacggtt gaggacttgg ttgaaagagg taagaaatta
      541 tcagtggatg taaaaattca gcgagaagaa atatttaatt caatgcataa tctgtagtag
      601 acagtaaggg gaggtgataa tagtgaatgt aggaaatata cttgaaacaa taaagatgat
      661 agatgaagaa cagttggata taagaactat aactatgggg atttctttat tagattgtat
      721 agaccctgat ggagataaag ctagagaaaa aatatacaat aagattatga gttctgctaa
      781 agatttggtg aaggttggaa aagatataga gcgtgaattt ggtatcccaa ttgtaaataa
      841 aagggtatca gtaactccaa tttccattat tgcaggtgca acagatgaaa atgactatgt
      901 aaagtttgca cagacattgg ataaggctgc agaggattta ggtatagatt ttattggtgg
      961 attctcagca ttagttcaaa agggatatac aaaaggtgat aagatactaa ttaagtcaat
     1021 tccaaaagct ctagcaagta ctaataaagt atgtgcttca gtaaatgtag gttctacaag
     1081 atgtggtata aatatggatg cagtgaaaga aatgggagaa atcataaaag aaactgcaga
     1141 attaacaaaa gatgcaaaag gttttggttg tgcaaaatta gtagtatttt gtaatgcagt
     1201 agaagataat ccatttatgg caggagcttt ccatggtgtt ggtgaggctg atagaattat
     1261 aaatgttggt gtaagtggcc ctggtgttgt taagagagct ttagagaaag taaagggaga
     1321 gccttttgat gttgtttcag aaacagttaa aaagactgca tttaaaatta caagagttgg
     1381 acaacttgtg gcaaaggaag cttctagtag attggatgtt ccatttggga taatagactt
     1441 atcactagct cctacacctg ctattggtga ttcagtagca aatatattag aggaaatggg
     1501 tcttgaagtt gttggtactc atgggactac agcagcatta gcacttttaa atgatgctgt
     1561 taaaaaaggt ggagtaatgg catgttcaca tgttggagga cttagtggag cgtttatacc
     1621 agtatctgaa gatgcaggta tgattgatgc tgttattaaa ggtgctttaa gtatagataa
     1681 gcttgaagca atgactgcta tttgttctgt aggtcttgat atgatagctg tgccaggaaa
     1741 tactacagca ggtacgcttg gagctatgat agctgatgag gcagcaatag gtatgattaa
     1801 taataagact acagccgtta gaattatacc agccccagga tgtgatgtag gtgatatggt
     1861 agaatttgga ggacttttag gacgagcacc agtaatgcct ataagtacaa attcatctga
     1921 attatttact caaagaggag gaaggattcc agctcctata cattcattta aaaattagtt
     1981 atattacata taataatatt taattaaata tttagatggt taaattaaag aagtctatgc
     2041 atttaaatat tatataagat aatgaacttc gttaagataa taaactttaa aaatcagttg
     2101 ttaagattat ggcaaaaata aagcgataca tggacattta aaaattcatg tatcgctttt
     2161 attttaataa tatctaatta atataaaatt ttttatgtat ctaaaaagtt atgctatcta
     2221 actaataata aactatacta
//
LOCUS       DAEPLF010000336         2237 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.338, whole genome shotgun sequence.
ACCESSION   DAEPLF010000336 DAEPLF010000000
VERSION     DAEPLF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.338"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(125..1510)
                     /locus_tag="KR314_003545"
     CDS             complement(125..1510)
                     /locus_tag="KR314_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0329896.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(1618..2022)
                     /locus_tag="KR314_003546"
     CDS             complement(1618..2022)
                     /locus_tag="KR314_003546"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329897.1"
                     /translation="MFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIAK
                     INYNKNGETYGTHIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAV
                     LYTRKRNFNIFKKTIPLYDKEGIKIIGRFEIN"
BASE COUNT          707 a          340 c          284 g          906 t
ORIGIN      
        1 ttatctattt gttattatga tgtaataata catattaatc tctataagtt aattactatc
       61 tggtgtaatt ttattttaac aacaaaaaaa aatacacaca attaaaatat tgtgtgcaat
      121 acaattattt tttctcttct aatagtcttt ttaatacgct cagaccagct ttaagttctt
      181 ctgtgctttc tgtagcacat acagctattc ttattgcaga aattggtttt tcttttccaa
      241 cagcaaaacg ttcagaagca tagacctgta caccttcttt aagtgcttgt atctcaaatt
      301 tttctgccat tattccatca ggtagaatca accatctaaa tatacattct tcatttccca
      361 aaagattata tccagataaa tattgattaa taatcttgtt tcttcttctt gcagatttac
      421 ggcgtgcatt aattaattta tcggcttcac ctgatgtaat catgcgataa gcaatttctg
      481 ttaaaaaata agattgagat agattaatgt tgtataatgc gcttgataat gcctctcgat
      541 actgtttagg tgtagagata tatgctagcc gtaaactagg tgcaataatt tttgataaac
      601 tcgtaatata aattgtttga ttgggcagat aggaagccac tggatttaaa tgtgtttcat
      661 taagtaaact atgaatagca tcctcaataa caattaaatt gtatttagat gcaatatttg
      721 caatcatttt tcgtccattt tgtgacatta tatgtgtagt tggattttga taatctggta
      781 ttatgtatag acctttaata ttttcatttt tacaagcata taatagtcct tcttcactaa
      841 tctcattatc ttcttgttta attggaatta gttgaatccc aagcatttta gctgctgtct
      901 ttattccagg ataagttaat gggtctacac ctattctatc tccatgttga aacaaaccag
      961 ctaaaatagc aacaattgca ttttgtccac cacttgcagg aagtaagcta tctgatgtag
     1021 tttcaagccc agctttataa attaatttgg caatagcttc tttttgccat ttagcaccat
     1081 ctctttgtcc atattggaat atagttttaa aatctatttc cttaattata tgtttaaaca
     1141 aagtattcat ttcttctagt gtgaaatctg gattcattgt gcccatttca ataaatgtaa
     1201 ttttattatt actggacatc aagaatagat ttgaacgcgt atcataagat acaaaagttc
     1261 cacttcctgt aactccactt attagacctt ttttttcaca aattttaaaa gctctagaga
     1321 tagtactcac attaacatct aagaaatctg ccaattcacg ttggggtggt aatttagtac
     1381 ctggtaataa aaatccattg ttgatatctt gttctagttg ttctgcaagc gccttatata
     1441 atatttgccc ttttgaaggt ctctttggtt tccagttcat aggataattt tcaaaagaat
     1501 taattggcat aagttttcac ctcacattat taatttgatt ttttctttat ctaaaacaat
     1561 aaattctaaa gatacaaata aaaactgata ggaaataatt ctatcagttt ttgtctttta
     1621 attgatttca aatcttccta taatttttat accttcttta tcataaagag ggattgtttt
     1681 tttaaatata ttaaaatttc tttttcttgt atataaaaca gcttcttctg gtgtctttgg
     1741 ttgattatca caatcgtaca aatctctttt ttttacgtag ccgacttttt tacctctacc
     1801 tattgtttgt ataagttctg gttcaatgga atttccattt gagtctatta tatgtgtacc
     1861 ataggtttct ccatttttat tataattaat cttagcaata gtatattcta catcttcatt
     1921 ttgtttaaaa ttggagttga tagaaaatat tgaccctatt attataaagg ttaataaaaa
     1981 cttaagacct aaattaattt tctttttatc ttgattaaac atagaattta acctcttttt
     2041 taaaataata ttttttttat ttagaaactg agatacgaat gatgaatact tcaaagtttt
     2101 ttgatacttt attgtattta atattatata tgcatactct ttaatttcac tcgtggtaca
     2161 tttgtcaatc acaatttcat cacaagataa ttcacataat tcatgaaaat atttttttaa
     2221 taagtataaa attggat
//
LOCUS       DAEPLF010000337         2233 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.339, whole genome shotgun sequence.
ACCESSION   DAEPLF010000337 DAEPLF010000000
VERSION     DAEPLF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.339"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..>2233
                     /locus_tag="KR314_003547"
     CDS             <1..>2233
                     /locus_tag="KR314_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0329898.1"
                     /translation="TKKNDVLLYDGNIYLLSHKKAHDYSKIYQILSKTKELKFDKEDS
                     KDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMIDGNICCDVKYIYDDEDK
                     KFVLPNIQKETTIENKLTSSQFTKENNHYVFKGTDYDLFTFLDLQLESLKEFGDIYYS
                     EKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYKDILKAFQEKRTFYKLKD
                     GNFIDLSERETKDFFELVENLDIMDKSKNNKIYKNKALYINDVIRSKNLKFIDGKKEL
                     DSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDYYKFGGILADEMGLGKTI
                     QTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLLIHGNKRDREKCFMELEN
                     FDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALVTESVKSINAENKFALTG
                     TPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKNLKKLIKPFILRRSKKQV
                     MKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLKKDKIVIFSYLTKLRQLC
                     LDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFTSVLKNISKELDKYKIKY
                     HYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNLTSADMVIHFDPWWNPSV
                     ENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKELINQFINGELSNEGVLK
                     SLSD"
BASE COUNT          968 a          225 c          321 g          719 t
ORIGIN      
        1 tactaaaaaa aatgatgtac ttttatatga tggcaatatc tatttattat cacataaaaa
       61 agctcatgat tattcaaaaa tatatcaaat attatcaaag actaaagagt taaaatttga
      121 taaagaggat tctaaggatg ttttaggatt gctagttcct agattaaaat ctatatctca
      181 agaagttcat ttagatgata acataaaaaa taatataaca aaagatttta aatctgagtt
      241 ctactttgat atgattgatg gcaacatatg ttgtgatgta aagtatatat atgatgatga
      301 ggataaaaaa tttgtgctcc ccaacataca aaaggaaact actattgaaa ataaacttac
      361 ttcttcacaa tttacaaagg aaaataatca ttatgtgttt aaaggaactg actatgacct
      421 atttacattt ttagatttac aattagaatc tctaaaggaa tttggagata tatattattc
      481 tgagaagttt aaattgaaaa aaatatataa cgcttcatca attcaagctt caatcaatca
      541 gactaaccag aattacctag aatttaattt taatatatct gatataaatc caaaagagta
      601 caaggatatt ttgaaagctt ttcaagaaaa acgtactttt tataaactta aagatggaaa
      661 ctttatagat ttatcagaaa gagaaacaaa agattttttt gaattagttg aaaatctaga
      721 tataatggat aaatcaaaaa ataataaaat ttataaaaat aaagctctat atataaatga
      781 tgtaatacgt agtaaaaatt taaagtttat agatggtaaa aaagaactag acagtatatg
      841 tgaaaaattt agaaattttg atagcataaa tttagaaatt cccgcaaatt taaatgcaaa
      901 ccttcgtgat tatcaaataa atggcctcaa ttggtttaaa gttttagatt attataaatt
      961 tggtggtata ttagctgatg aaatgggtct tggtaaaaca atacagacta ttgcattttt
     1021 attatcatta tctaataaaa aaagtcttat tgttacaccg acatcactca tttataattg
     1081 gaaaaatgaa tttgaaaagt ttgctccaga tataaaagtt ttattgatac atgggaataa
     1141 aagagataga gaaaaatgtt ttatggaatt agaaaatttt gatgtaatac ttacaacata
     1201 tggtactctt agaaatgatt tagaaaaata tagtgaaata aagtttgatt attgtatatt
     1261 agatgaagct caaaatataa aaaatccagt agctttagta actgagtctg taaaaagtat
     1321 aaatgcagaa aataaatttg cactcactgg tacacctatg gaaaataatc tactagaatt
     1381 atggtctata tttgatttta taatgcctgg ctatctatat agtaaagcaa agtttcagga
     1441 attgtttata aataaagagg ataatgttaa aaacctgaaa aaattaataa aaccttttat
     1501 tcttagaaga agtaaaaaac aagttatgaa agaacttcca gataagattg aaaaaaactt
     1561 ttttgttgaa ttgaataaag agcagaaaaa gatatatagt gtatattcaa aagatataca
     1621 agataaaatg aaagataaaa atctaaagaa agataaaata gttatatttt cttatttaac
     1681 taaactaaga caactttgtt tagacccaag tatagtagta aaagattaca ataaaaaaag
     1741 ttcaaaaatt gaaacatgtt tagaaatact tagggatagt ataaacgaaa accacaaaat
     1801 attattgttt tcacaattta cttctgtgct taagaacata agtaaggaac ttgataagta
     1861 taaaattaaa taccattata tagatggtaa aactaatgcc aaagaaagac ttgaacttgt
     1921 agatgagttc aataatagta tggataaaaa agttttttta atatctttaa aagcaggcgg
     1981 aactggttta aatcttacat ctgctgacat ggtaattcac tttgacccat ggtggaatcc
     2041 atcagtagaa aatcaagcta gtgatagagc tcatagattt ggtcaaaaaa attcagtaca
     2101 agttataaaa ttgattgcta aagggactat agaagaaaaa ataataaaat tacaagaaag
     2161 taaaaaagag cttataaatc aatttataaa tggagaactt tctaatgaag gtgtattaaa
     2221 aagtctatct gat
//
LOCUS       DAEPLF010000338         2209 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.340, whole genome shotgun sequence.
ACCESSION   DAEPLF010000338 DAEPLF010000000
VERSION     DAEPLF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.340"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..240
                     /locus_tag="KR314_003548"
     CDS             <1..240
                     /locus_tag="KR314_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0329899.1"
                     /translation="FVRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNI
                     REWICPSCNEIHDRDINASINILKEGLRLITIQNK"
     gene            524..970
                     /locus_tag="KR314_003549"
     CDS             524..970
                     /locus_tag="KR314_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBH0329900.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            991..2034
                     /locus_tag="KR314_003550"
     CDS             991..2034
                     /locus_tag="KR314_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBH0329901.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT          903 a          231 c          422 g          653 t
ORIGIN      
        1 tttgttcgtc aattagaata caaggctaat tggcatggta gacaaattgt aaaggtaggt
       61 aaattctttg caagttcaca aatatgcaat aaatgtgggt ataaaaacga ggaagtgaag
      121 gacttaaata taagagaatg gatttgtcct agttgtaatg aaattcacga tagagatata
      181 aatgcaagta taaatatatt aaaagaagga ttaagactaa taacgattca aaataaataa
      241 cttatgtaag aaccgtagga actacgggga tagcctgtgg agaattagta agacatatgt
      301 tagtatgcaa aattctatga agcaggaacc ctgtgacttt agtcatggga ggttcagact
      361 actttgtaac aattttaaca aagtatgttg atataatact attaaattaa tcttagatta
      421 gtttattcta ttgatttgta gtttaaattt atattcaaaa acattgtata aacatataat
      481 ttatataggg ttttataatt catatgaaaa gagggtagat agtatgaagt taatatggtt
      541 gatagtagca gtcttatttg gaatagcaga aatgttaaca ccaagcttaa cattgatatg
      601 gtttagtgta ggagctgtaa tacttatatt tttaagtagc tttatagaga gtattttctt
      661 acaaatttta atatttgcag taatttctat agcaatgtta gtcgtagcta ctaaaaaaat
      721 agtaaaaaaa gataaaggct ataaatctaa cactaattta caagcaatga tgtctaaaaa
      781 aggtattgtt acagaagaga tttctccaaa taacacaggt ctagttgtag ttgaacatga
      841 aacatggacg gctatttcta tagatggtga aaagatagaa aaaggttcta cagtagaagt
      901 attaaaaatt gaaggggtaa aattagttgt taaaaaagta gacgccacag ttaatgtgac
      961 aaaccaatag aataaaggag gatttatagt atgggtacta aaattgtttt aagtattgta
     1021 ttaatagtag tagtcgtagc aataagttta acgtgtataa gagttataaa acaatctaaa
     1081 gtaggtataa taatgagact tggtaagttt caaaaagtgg ctgaaacagg tgtacatttc
     1141 ttaataccat ttttagataa aatggcatat gtaattgacc ttagagaaat tgtaatagat
     1201 tttccacctc aaccagttat aactaaagat aatgtaacta tgcagattga tacagttgta
     1261 tattataaag ttacagatcc agttagatat gtgtttgaga tagctaatcc aatagctgct
     1321 attgaaaatt taacagctac aacactaaga aatataattg gtgaacttga tttagatgaa
     1381 acattaacat caagagatat aataaatgta aaaatgagaa ctatccttga tgaagcaaca
     1441 gataaatggg gtataaaagt aaatagagta gagttaaaaa atataatgcc tcctcaggat
     1501 attcaagttg cgatggaaaa acaaatgaga gcagaaagag aaagaagaga ggcaatactt
     1561 caagcagaag gtaataagtc agctgcaata cttcaagcag aaggtgaaaa acaatctgcg
     1621 atattaacag cagaagccaa aaaagaagct atggtacgtg tagcagaagg tgaaaaagaa
     1681 tctgctatat tagtggcaga aggtgaagct gaagctataa gacagaccgc tattgctaag
     1741 gcacaaggtg aggctgaaat gataaaaaga actcaaatgg caacagcaga aggtttaaaa
     1801 ttagtttttt cagcaatgaa agaggctgat atagacaata atattctagc attaaaatct
     1861 atggaagctc ttgaaaaaat ggctgaaggt aagtcaacaa aactggtttt accttcagaa
     1921 gcagttaatt tcctaggaac attcaaagga ataaaggaag ttatgagtga tgataacaaa
     1981 gaagtacttg atataaaaga agttttaaat gataatgaat cattaaaaaa ataaaagagt
     2041 tttgttatca taggaaggat atggaaatta aaggtcgaat taattaaata tagcacaaaa
     2101 atgcacgttg aaaagaagag gacttataaa tttttaagtc ttcttctttg tttttattag
     2161 aaagatgaaa ttataaaaat gaattaacag aatctttata caatttatc
//
LOCUS       DAEPLF010000339         2191 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.341, whole genome shotgun sequence.
ACCESSION   DAEPLF010000339 DAEPLF010000000
VERSION     DAEPLF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.341"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..649)
                     /locus_tag="KR314_003551"
     CDS             complement(<1..649)
                     /locus_tag="KR314_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329902.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKV"
     gene            764..2179
                     /locus_tag="KR314_003552"
     CDS             764..2179
                     /locus_tag="KR314_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0329903.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNSKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQYVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
BASE COUNT          806 a          254 c          334 g          797 t
ORIGIN      
        1 taacttttaa atctggaaaa gcacttgaaa tatagtgttt tatgtcattt aaatggtctt
       61 gaatttgctt ttctgatatt ttatcaggtc catatacaaa atacataata tttttagtat
      121 cttttgttat agctgttcga ttatcagggc cattaagaat actagataat ataccaatat
      181 tatctgataa aaataaatca tcttttgtta aaatatgctc tttaccacct ataccataaa
      241 aatgttcact tccatttgct aattctattt tcaaaggtaa ttccatttta tcaaggtcat
      301 gacctgctgt tagaagaaga tttttcaatt ctgctaaaaa cattgactca attgttacac
      361 ctacatctgg gatacctttt gattttaata gaatagattc taattgaagc aatacagggt
      421 aagttttctt gaatttttta taatacttta tataacttga aacaggctct gttttaattt
      481 tttctttacg attgtattct gggtgttgtt ctataatatt tttaattgta gagtttttaa
      541 gaactaaaaa attttcttta ttaggttttg aatgtgtaat atttataacc ataactccaa
      601 atttaatatt gggataaatt tctttaactt tatgtgatat ttctaacata atatagactc
      661 ctttttattt tatattttat gatataatct tagcaagtta aaagtggatt ttgtagttcc
      721 atttttatag taattttata gaaccacttt tggaggtatt agaatgtgga ttacgattaa
      781 tagaaatagt aatatatcat tatcaagaca aatttattgt gagataaaag atatgattct
      841 ggatggaagc ttaaagtttg agcaaaaact gccatcatct aggactttat caaaagaact
      901 atctgtatct cgtaatacag ttttagatgc atataatcaa ttaattgcag aaggatattt
      961 ggaaacaagg aatggatatg gaacaattgt agctaaagga atatcagggc ataaagttga
     1021 ttttaagtta aatgatactt ttagagatga aaaagattta catactaata aacaaaaaaa
     1081 ttatatagat tttcgttcag gagttccaga attaaaacta tttccacgca aagaactttc
     1141 aaagctatat aaacaaatac tgtatgattt atcagattcg gatttcagat atggaagtac
     1201 agctggtgta tgggttttaa gagtggaaat atctaagtat ttatttcgta ctagaggaat
     1261 tagatgtaat tccaaaaaca ttatgattgt ttctggttca acacaaggtt tatctttaat
     1321 ttcacagctt ttatacaaaa gtaataaaaa ggttgctgta gaagacccta ttcataaagg
     1381 cttattaaag gttatttcat ctattggata ttcagttgtt ggaattaaag tagatgataa
     1441 gggaatgaat accaacttaa taaaatcaat aaaggggatt tcttttgttt atactacacc
     1501 ttctcatcaa tatccattag gagggatttt acctattcaa agaagactag agcttattaa
     1561 atgtgcaaca gagaatgatt actatataat tgaagatgat tatgatagtg aatttagata
     1621 tgagggtcaa ccaattagtt ctctttatga acttagccca aataaagtaa tttacatagg
     1681 ttcttttagt aaaatattat ctccagctat tcgtgttgga tttatgttat taccagacaa
     1741 tattttagaa agctataaaa ccttaaaaat gtactctgat gttcatacag aaacaatatt
     1801 gcaatatgta ttagctgaat ttattggaaa tggaggttta gagaagtaca tatttagaat
     1861 gaagaagttg tacaatagaa aacgtaaata tctcataaaa gttttaaaca cggaatttaa
     1921 aaatgaattt aaaataaaag gtgaagcagc agggcttcat atagttgctc atttctataa
     1981 tgttaaattt acggaaagtt taattcataa aatttttgaa gcaggtataa aagtttatcc
     2041 tatacaacaa tatgcaattg agaatatatt agaatatgac catgaaatta tactaggtta
     2101 tgctcatctt agttttgatg aaattgaaaa aggtgtaaaa atactcagca aaataataca
     2161 tatgaatcaa ggtacataga taaaacatgt a
//
LOCUS       DAEPLF010000340         2189 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.342, whole genome shotgun sequence.
ACCESSION   DAEPLF010000340 DAEPLF010000000
VERSION     DAEPLF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.342"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..195)
                     /locus_tag="KR314_003553"
     CDS             complement(<1..195)
                     /locus_tag="KR314_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329904.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILI"
     gene            complement(413..1369)
                     /locus_tag="KR314_003554"
     CDS             complement(413..1369)
                     /locus_tag="KR314_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBH0329905.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTIYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIQAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            1810..2178
                     /locus_tag="KR314_003555"
     CDS             1810..2178
                     /locus_tag="KR314_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBH0329906.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNKVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT          777 a          303 c          297 g          812 t
ORIGIN      
        1 tattaatata ttttctatct ttttaaatat ttctatattt gctggtatta tactgagctc
       61 aatattactt ttaaacgggt tagactgtaa atctgttata agtatatttt ttaattcctc
      121 tgtaagttca gaaattaaac tatcacctat gtattttttt atgatttcct ctctaatttc
      181 ttttaaattt agcaatatta gtacctgcta cttacatagt agcttttcct ttccttttaa
      241 tactattatt catttttgaa tcttatttag atatatttta agattaaaag tctttagcta
      301 tataattttt tacccatttt taacttatat taacatatta caggcttata tctccaccct
      361 gcttacattg aggatggagt atattgccta ttttgataaa aatgataggt tgttatttgc
      421 ctattccttt ttctgataat ttttctaaca tatctttggt cattttatct aaatcataac
      481 taaacttaaa gttccattct tttactgcac tactactgtc tagagagtta ggccaagaat
      541 cagctatact ttgtcttaca gggtctacat catattctat cacaaaatca ggaatatatt
      601 tttttataga atcagctatt tcttcaggag aaaagctcat agcagtaata ttaaaagcat
      661 ttctatggat gagtttatcc gctggagctt ggattaaatc cactattgat tgaagtgcat
      721 caggcatata catcatatcc atttttgtac cttctgctat gtaagattta tatctcttat
      781 tttttaatgc ctcataatat atatcaactg cataatctgt agttcctcca ccaggaggcg
      841 taacataaga aataagacct ggaaatctaa cacctctagt gtctactcca aattttgagt
      901 gataataatc acatagtaat tctccagaaa ctttagtcac accatatata gtattaggtc
      961 tttgtaatgt atcttgagga gtcatatttt ttggagaatt ttcaccaaaa gcagcaattg
     1021 aactaggtgt aaaaaattta caatccaatt ctttggctat ttcaaggcca tttattaatc
     1081 cattcatatt taagttccaa gcttctaggg gtttactttc agcaactgca gaaagtaaag
     1141 atgctagatg aattatagta tctacatcat atttttttgc aatttcaaaa aaacgattct
     1201 tatctgtaac atctaatatc tcaaatatac cagattcaca tactggatta ccttctttta
     1261 ctctccttga actagcgatt acattttgtt ctccatactc atttctaagc tttattgtta
     1321 attctgaacc tatttgtcct aatgcacctg ttataagtat ttttttcaat tttacaacct
     1381 cctaagggtt taatttatgt agtactactt atttttattt tagcaaatct tttgtttaaa
     1441 tacaaagaat taaagataat aatatacgac ttaattgaat gaagtgcagt aaattcctaa
     1501 aaatttaaag ttgagatata atggtctatt gtataacaat aaaagactag attaaattat
     1561 tatatagatt gtgtgactaa gtcacagaaa gtatagttaa tttaatatat aataaaagca
     1621 tatcaagtaa agctttaaat taaagtaact ttatataaaa agtaatataa attgataaaa
     1681 ttataaattt atttatagtg aatataggtt ggattttata gatacaaaaa tagtatatat
     1741 gatatgtaga ccattagcat gtttaaagta gcaaaacata taaaataaaa tttgaaagga
     1801 agtataaaaa tgacaagacc atcaatatat catagccaag gatacacttg tgcagaggca
     1861 ttaataaagt cttataatga ggaacacaac acagatatac caatctcaat aggaagtggt
     1921 atgggggttg gaatgaatgt tggaagtgtt tgtggagctg taaatgctgc tgttgttata
     1981 atagggtatt taaatgggcg aaatagtaat ttagatgaaa atatagcaag gaagtattca
     2041 agagagctat tgaataaagt aagaaataaa tacagctcag aaatatgtgt atctcttaaa
     2101 aaaaataaag taacttgtga agaaataata aatttcacat atgatgctct aaatgatatg
     2161 ctaaaaaatc aaaaataatt ctaagaaac
//
LOCUS       DAEPLF010000341         2172 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.343, whole genome shotgun sequence.
ACCESSION   DAEPLF010000341 DAEPLF010000000
VERSION     DAEPLF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.343"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            200..1819
                     /locus_tag="KR314_003556"
     CDS             200..1819
                     /locus_tag="KR314_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBH0329907.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGNCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
BASE COUNT          798 a          269 c          318 g          787 t
ORIGIN      
        1 ttaaattgct atatatcata aattttctaa aattaacaac aacttaaaaa tttaacattc
       61 tcttaacgta tacttaacaa aacattaata tttctattga caaaatttgt tattctatat
      121 tatggaaaca ataaatttta gttaataaag tttataattc agataaaaaa actattaatt
      181 tcttaaggga ggaaatatcg tgaatatagt aattagcctt atcggtggac ttggactttt
      241 tctttacgga atgagtttaa tgggagaagg tttacaaaaa tcagcaggag acaaattaaa
      301 aaaaattatt gaactcttaa caagtaatgt tgttatgggt gtactggtag gtacagtcgt
      361 tacaggtatt atacaaagtt caagtgctac tactgttatg gttgtaggat ttgtaaatgc
      421 aggtataatg aatctaagtc aagctattgg tgtaataatg ggggcaaata taggtactac
      481 agttactgct caattagttt catttaatct tgaaggaatt gcaccaattg ctttaggtat
      541 aggtatttta ttctatttat ttacatcaaa tcaaaaaaca aagcatttat ctgagatact
      601 tataggtttt ggtatattat ttacaggaat ggagtttatg aaagatgcag tagctccact
      661 tgctgaatat aaagcattca ctgatgcact tttatatttt agtaaaaatc ctgtattagg
      721 tatattggca ggatttgcca taactggtat tgttcaaagt tcaagtgcat ctatgggtat
      781 gttaatagca ctagcttctc aaggaatact tcctctatca tctgctttac ctattcttta
      841 tggagataat ataggtactt gtgttacttc acttttatca agtgttggtg caagtagaaa
      901 tgctcgacgt gcagctgtaa tgcatttaag ttttaatgtc attggtacaa taatatttat
      961 gcttgtttta aataaaccta tatcagccat agttactcat tttgacccaa ctgatacagc
     1021 tagacaaata gcaaatgcac atactttatt taatcttaca aatgttataa tattattacc
     1081 attttctaaa tacatagtta aattagcaaa tagacttata cctattaaag aaactgaatc
     1141 agaaatagta aataatacta agtacttaga tgaaaggatg tttagcactc catctatagc
     1201 acttggtaat actgtacaag aagttgtaag aatgggtcat aaagctacaa actctttgga
     1261 acattcaatt gcaggatttt taaataagag caatgaagat attaataaag cttttgagtc
     1321 agaaaaagtt gtcaataagt tacagaaaga tatattgaac tatttgttaa aactttctaa
     1381 agaacctctt agagacgatg aacgctttag aacagatttg ctttttaata ctgtaaatga
     1441 cattgaaaga gtctctgacc atgcagaaaa tatagctgaa ttggccatga gtgttaagga
     1501 aatgaacatt agtttttctg atagtgcgat aagagaaata tacgaaatat acaataagac
     1561 aattactaat tttaaggatg ccttaatagt tttagatgtt aaagattttg agttagcaaa
     1621 taaagtttta gaagttgaaa atgaagtaaa ttatttagaa aaaactttta gaaattcaca
     1681 tatgattaga ctaaataatg gaaattgtac aattgatgct ggtgtacttt atcttgactt
     1741 acttactaat ctagaaagaa tttctgacca ctcaacaaat atagtaaaac aagtattaaa
     1801 acttaaacaa aaaatatagt gattattttt atatataatc ttaatcaatg agaagtaaga
     1861 agctatagat aatgcttata tcatttatgg cttcttattt tttatgtaat actcaaattt
     1921 ttatagtatt ctcttattat taaacagctt ctgcctagaa tattccatat atatatgact
     1981 tctttcataa aacttgaact gcccataact aaagtcacaa gtatacataa ctatttttta
     2041 atcaattata accaatattt attttattta tcttcatttt aattcttcat tcaataatgt
     2101 aataagatta ttttttattt ggatactatt atatcatcta aatatgttat tatatatttg
     2161 gatattttca ta
//
LOCUS       DAEPLF010000342         2141 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.344, whole genome shotgun sequence.
ACCESSION   DAEPLF010000342 DAEPLF010000000
VERSION     DAEPLF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.344"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(56..1255)
                     /locus_tag="KR314_003557"
     CDS             complement(56..1255)
                     /locus_tag="KR314_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBH0329908.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            1374..>2141
                     /locus_tag="KR314_003558"
     CDS             1374..>2141
                     /locus_tag="KR314_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0329909.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKL"
BASE COUNT          742 a          340 c          279 g          780 t
ORIGIN      
        1 taaattttca tgttaatata tataatccaa aaaaattaaa tcttattaaa acatactatt
       61 ttgtatagtt atcgaaatat ccttgtatgt aaataatagg agtaccctta tctccacttc
      121 cagatgttaa atctgctaaa gaacctataa ggtctgttaa tcttcttgga gtagtacctt
      181 gagaaaccat gtttcctgtt aaatcatcag atttattatc tttttcaact atgtattttg
      241 aaattgcttc ttttaactca tcaccagata agtcagcaaa atcattatct gctaagtatt
      301 ttaattttac ttcgtttgga gttccctcta gcccttttgt ataagcagga gatactactg
      361 ggtcagctaa ttcccatatt ttacctacag ggtctttaaa agctccatct ccataaacca
      421 ttacttctac atctttccct gttatttctt ttatattacc ttggattttg tttacaactt
      481 catcgcaatt tatagggaat aattttactg tttcttctgt agctttgttt gaacctaaaa
      541 gtccatactg gtcattgtaa ccactaccat ctacacttgc tgtcattata tcatcaagtg
      601 aatatacttt tttagctcca ttttgtctta aaattctctt tgttctttgt cttgtatgta
      661 tatcacaagt taaaatactt gttgtatagt taagaatagt ttttggattg tttgcaaata
      721 ctatttctac ctctgcacca caatccttga ttaaattctt ataataatca acataatcta
      781 caccagtaaa tgtatgcttg ttatatccaa atagttctct gtatttttct tctgttaaaa
      841 catctgacca aggatttatt cccttatcat caagttcatc aatacttatt aagtgatttc
      901 caacttcatc agaagggtag cttaacatta atacgacttt tctacatcct tttgcaattc
      961 cttttaaaca tatagcaaaa cgatttctac ttaaaattgg gaatataacc ccaacagtat
     1021 catctccaaa tttatctttt acatcttttg caatattgtc aacgtgagca taatttccct
     1081 gtgctctagc tacaacagct tctgtaacgg caattacatc tttgtcccta acttcaaagt
     1141 tttcactttt tgacgcattt aaaacagaat caacaactat tttaactaag tcatctcctt
     1201 gtcttataat aggtgctcta acccctcttg aaacagttcc aactaatcta tccattatct
     1261 aaaatcctcc taaagaatta atattgtcat attagtacca tgtatattat aattcaaata
     1321 taattataag taaaataaat aatactaata tttgacataa ggagtaatta tatatgaata
     1381 taaaacttga actttataag gtttttaatg cagttgtaaa taataagagt ttttctcttg
     1441 ctgctaagga attgtttatg tcacagccag ctgtaagcca gtcaataaag caattagaag
     1501 aacaattaga tactttatta ttttatagaa acaataaggg agtaaaatta actccagagg
     1561 ggaaaattct aagtgaacat gtcactactg cattaaagtt gatatcatca ggagaagata
     1621 gaataaataa atttaaaaaa ttagaatacg gttctctgaa aataggagta ggagatacag
     1681 ctgcacgttt tttcttgtta aaatatttag aaatttttca taagaagtat ccacatatac
     1741 atgtatctac aataaataga acgagtagag aacttatatc tttactcaaa gatgggaata
     1801 tagatatagc aattataaac atgccaatag aagatgatac actaaatatt gttgagtgta
     1861 ttgaaataca tgatatattt gtatgtgcaa atgattacat agaatacaaa ggtaggaaaa
     1921 tatcattgga agaattaaac acattgcctt taataatgct agaaaataaa gcaaattcaa
     1981 gactttatgt aaatgaatat tttttatcaa aaggtataaa actaaacccg gatatagaat
     2041 tgggttctca tgaattactt ttagaatttg cgtatataaa tcttggagtg tcttgtgtta
     2101 tagaagaatt tagcatagat tatttggaaa atgagaaatt g
//
LOCUS       DAEPLF010000343         2140 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.345, whole genome shotgun sequence.
ACCESSION   DAEPLF010000343 DAEPLF010000000
VERSION     DAEPLF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.345"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(147..686)
                     /gene="scpB"
                     /locus_tag="KR314_003559"
     CDS             complement(147..686)
                     /gene="scpB"
                     /locus_tag="KR314_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBH0329910.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            complement(688..1407)
                     /locus_tag="KR314_003560"
     CDS             complement(688..1407)
                     /locus_tag="KR314_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBH0329911.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            complement(1437..2033)
                     /locus_tag="KR314_003561"
     CDS             complement(1437..2033)
                     /locus_tag="KR314_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBH0329912.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLIYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
BASE COUNT          690 a          346 c          189 g          915 t
ORIGIN      
        1 aaaaaattac tatttatact taattatcca tatttatttt aagagaatta taattttata
       61 tttatacaaa aaaacacaac aacttagagc attttaaatc ttttaaaatt taaaattaca
      121 tactaaatta ttgtgatttt atatttttat tcatttgttg ccacttcttg ataattttct
      181 ataggcggca attcttcaag actttctata ttcaacaact ttaaaaattc atctgttgtt
      241 ttataaatta ttggtttccc tatcttattc aatctaccag cttctcttat aagttcattt
      301 tcaaacaaag tttgtaatac cttatcacat tttactcctc ttatatcctc tatttctacc
      361 tttgttattg gttgtttgta ggcaattata gtaagagttt ctaatgtagc ttggctcaaa
      421 cttttctttt tctttggttc aataatcttc tttatatatt ctgcatagtc tttatttgta
      481 cacatttggt acttattctc tagcttgatt atttgaatac ctctattttg ttctctgtac
      541 tcctcaataa gtaaatttaa cataatctct atttcttttg atgaaagttc tttatttatt
      601 atatagttta attcttttat acttattggt tccccatatg caaacataac agattctata
      661 atatacttta tatcttctct tttcatttta acactccgaa ctttttctta ttagaatatc
      721 atcaaaaaat atgtcttgta ctactactat ttctttttct tttataagct ctaatataga
      781 taaaaaagta gctattattt catcattttc atatgatttt ataatatttg taaaagtaac
      841 ttcaatctta tcttttataa tatctcttat atacaatatc ttttcttcca cagatattat
      901 cttcttcctt actattttgt ctagtttctc atcattagca ttttctatct gtgatgtttt
      961 aactttaaat atgtatggaa gtatattttt tattgcttca attgatatat cttctaaatc
     1021 cacattgtca tcaataatta tctcttcttt atttctataa aatctctcat taacataagt
     1081 tatattttct tttatatctt gtgatgctac cttaaacttt ctatattctt ctaacttttc
     1141 cataagttct attcttgggt cttcttcttc atctttttgc ttataaagca gatatttaga
     1201 ctttatctct aaaagttttg atgccatagt tataaattca ctagttatct ctaaatccat
     1261 tttatctagc attttcaaat aatttagata ctgcttagtt atatctatta tagatatatc
     1321 ctttatatct attttatgct ttgttattaa atcataaagt aaatctaatg gtccctcata
     1381 gacttgtaat tgtatattat acttcatatc aatccctctt taatctgttt attatattaa
     1441 acaaataaat atactatttt tagcaacact gtatatattg gtgatagtat tatttgatat
     1501 ataccagtaa ataaagcaat taataaaatt atataactca tactttcaaa tttatacaaa
     1561 tattcattcc atttatatgg tacaaatgta gatataactc cccatccatc aagtggaggt
     1621 atcggaagta agttaaaagc tgcaaatccc atactatata taagcacata tccagctatt
     1681 gtatttatag catacatatg cacatactta ttgataatta cacacagtat agcagtcaaa
     1741 acattacaaa aaacgcctgc aagtgaaact atcaggtttc ctatcttata gtttttaaaa
     1801 ttatttggat ttactggtac tggctttgcc caccctatct taaggataaa catagccaaa
     1861 agacctattg gatcaacatg tcttacagga tttaatgtca ttctaccata agctttagct
     1921 gtatcatctc ccaataagtg agctgaataa gcatggccaa actcatgtat tgatattgcc
     1981 atagctatac caactaaact tgctactatt tctcctatat tcatacttaa cattaatgta
     2041 catctcctaa attaaatttt taatattatt ataacaatta aatagcttat aatcaaaatt
     2101 attttacatt ataataattt taacttctag gcataaaatt
//
LOCUS       DAEPLF010000344         2136 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.346, whole genome shotgun sequence.
ACCESSION   DAEPLF010000344 DAEPLF010000000
VERSION     DAEPLF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.346"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(80..1372)
                     /gene="brnQ"
                     /locus_tag="KR314_003562"
     CDS             complement(80..1372)
                     /gene="brnQ"
                     /locus_tag="KR314_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBH0329913.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIKFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          711 a          364 c          240 g          821 t
ORIGIN      
        1 tttaataatt cttttaaata taaaaaggaa ttgccatata atttaaaggc aattccacaa
       61 ttatcaatta gttttattat tatgcagttt tactgctttt agatggaata aagtttccta
      121 ttattcctgc tattacaact ggaactatcc agttaaatcc taatgaagca aatggtaagc
      181 tattaactag aggaattttt acaccaaaac ttgttgcaac tgttaataca cttactaata
      241 atgccatgta tgcagcacat ttgaacacgt tgtcattttt aattttatta ctaaataatg
      301 ctagtataac tagagttata gttgctggat ataccatact tagtatagga gaagagaatt
      361 ttattattgt acttactcca aagtttgata tgattgcact aaatacacaa actactataa
      421 ctatatgctc atactttaat tttccatctg taagtgttga gaaatattga ccagctgctg
      481 aagaaagacc tatagctgtt gttaaacatg ctaatgcaac tataatagcc aataaaattt
      541 tacctgtagt tccaagtaaa gctgctgtta ttgcaactat taaagctgtt tgttgaacat
      601 cttgtccata ttgttgagac acagttgctc caagataaca taatccacca taaactaatg
      661 ccattcctat tactgcaatt acacctgcac ttattgtggc tttcatcttt tctttctcac
      721 ctgtataacc tttagctatt actgacatga ttataattga tgcaaataca ctagctgcca
      781 atgcatccat tgtttgataa ccttgagtta taccttctgc aaatacattt tgaattaaag
      841 aactgtctct tatttctcct aatggagata cgataccttt aactattaat actgctaaag
      901 ctataagtaa agctggagtt aatattgacc caactatatc tacaactttt gatggtctta
      961 ttgttaaaac caatactaga gcaaagaata ctatagagaa tattactgga cttattccag
     1021 aacctattgt aggcaatata cccatttcat aagttgttgc tgctgttcta ggtattgcta
     1081 aaagtggccc tatacagatt accattgcac atcctaatgc aatacttaat cctttgcctg
     1141 ctcttgagaa catcttcgat gtgtctccat tgaacttagt tgctgctata actgctaata
     1201 atgccaaacc accatctgca aaaaggaatc ctatgaaagc tattagccaa ctacttccag
     1261 ataaaactcc tagatatgga gggaagatta agttccctgc accaaaaaac attgcaaata
     1321 gtgcaaatcc aattacaatt acatctttaa aattgatttg tttctcaccc atttaaagca
     1381 cccccatttg aattttacta ccttttacta aaaaataagt ccaatctctt cattaaatga
     1441 aaaccttttg acgagatttt atttaattat acatttttga cttaaatttg tcaattatgt
     1501 ttcataattt ataaaatctt cacaatattt aatacaactt tcttaattaa acaaaatcaa
     1561 cctgtatact ttaaacatag ttggtttttt gttttttctc taattttcct tgataaaatt
     1621 aactattcag aacaattaaa atattaagac tttttagatt attcgtttta ttattaagtt
     1681 gtattgcttt atatactgta tatatcactc tcctttttac attcatataa tctttttaat
     1741 tcaatttaaa ttttaaatat tttgtgttta ttatataatc tgaaaaaaaa gcagtcaata
     1801 gcatttttaa tttttttaat tttcaaaatt atgattctta aaaaatcgtt ttccttttat
     1861 cataacacct attaacgtta attcttgtag tattttgtat ttattttgct aaattgacag
     1921 ttaactttat tcaattttta ttctactcac gtagatttac ctcttgtctt ttagtcttaa
     1981 aaccattttt tctgaaaatt ccttattatg atttatcaac ttatttttct tcatagcaat
     2041 tactttctaa cttccaactt acttaaactt tgcagttgct agtattcacg tttacatact
     2101 tatatgtcat aattaactaa aaattcttaa tatatc
//
LOCUS       DAEPLF010000345         2078 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.347, whole genome shotgun sequence.
ACCESSION   DAEPLF010000345 DAEPLF010000000
VERSION     DAEPLF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.347"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            107..2005
                     /locus_tag="KR314_003563"
     CDS             107..2005
                     /locus_tag="KR314_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBH0329914.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
BASE COUNT          856 a          226 c          405 g          591 t
ORIGIN      
        1 aaaacacgaa taatttgcaa gaaaaattca tgaatgtaag gaaaatgaaa tttatcttgt
       61 atttaacaaa ataaaatata cataaaaatt atgggagagt ggaaaaatgg atagtctatt
      121 atacgtaata gagaaagata aacacactaa tgaagaatta agagaaatcc taaaaagcaa
      181 taaaaatata agatttgttt ccttaatggg ggttgattta ggaggaaatg caacagatga
      241 aaagattcct gtagaattat ttttggagga tatagataaa tttttggaat cagctataca
      301 gactgatggt tcaagtgttg agctttataa tatagctaca ttaaataatg cgaaagtaga
      361 tttaatgcca gataaaagtt gtcattggta tgtagattac aatatggaat atatagacga
      421 agaagtaggt ctacctgttg gaacattaaa gatacctgca tttttaatac atgataataa
      481 aaaagtatgt tctagaggag tacttcaaaa agcagataag tactttaaaa agtcaatgta
      541 tgaaatattt agagaatatc cacatgtaat aaataacata ggaatagatt cagtagatga
      601 tatagaggaa ataatgttaa cagcagctac agaacttgag ttctgggtta atactcctga
      661 ggataaagct gacttagaga aattatatgt atcacaaagc ctaaaagagc aatactggaa
      721 aagaactcat ggtattataa gaacttgttt agagaaaagt ttaattatac ttcaaaagct
      781 aggtgtaaac cctgaaatgg ctcacaaaga ggtcggagga atacaaagtt ctattagtat
      841 agatggaaga acaaaccatg ctatggagca attagaggtt tcttggaagt tttctacacc
      901 attacaagca gcagacaatg aattattagt aagagatgtt atagaggatg tatttacatc
      961 acatggacta gaagttacat ttaaagcaaa gccaattcat ggagtagcag gaagtggtgg
     1021 acatacacat gtaggagtaa gtgcgaagtt aaaggatgga agtataaaaa atctatttgc
     1081 tccaaaagat ttaaaagaag attatctaag tgaactagga tatggggctc ttatgggact
     1141 tctttacaat tatgaagtat taaacccaat tgtaacagct tctaatgatg gatttaatag
     1201 attagttcct ggatttgaag ctcctgtatg catagttaca tctttagggc atagttatga
     1261 gattccatca agaaataggt cagtgcttgt tggattaata agagatatga aaaatccaaa
     1321 gactgtaaga tttgagttaa ggtcaccaag ccctctatct aacacgtatt tagtaatagc
     1381 aggatgttat caaacaatgt tggatggtat aaaagcagca gctaagagtg gtcttagtac
     1441 aaaagaactt gaaaaagagt tatctaaaaa tgtaggtgaa gaaagtttct atttagaaaa
     1501 agatagagca tatagagatg aaaatgatgt atttgaacat tatagtttgg aagaaagaaa
     1561 tgcaagattt ggaataccac cggctacagt ttatgaaaat atgaaaaact tagaaatata
     1621 tgcttcaaaa ttaaaatctt taaaacaagg tgatgtattt actgatagta taatagaatc
     1681 atttaagatt ggagctatag ataagtggca aaaaaaatta aaaactagaa taatagaggc
     1741 tgggattcaa aaaataagaa gtattgtcaa gatacacaca aaagaaaata tggatgcgct
     1801 tgatgaggta gtttggaact caatatcgga tttaaagttc aatattatga aggatacttt
     1861 aactagagaa tcactattca ctagagtgag agaagctata gaaaataagg attaccagac
     1921 tgcttcagat ttacaaatag aattaaagag aagtatggaa gaaatacaac aactgtatat
     1981 gcagtacaag aaaaatatat actaatataa caatttgatt ttgatatgat aaaggcaatc
     2041 aagtattatt aaacactatg agtaaattct caagtgta
//
LOCUS       DAEPLF010000346         2062 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.348, whole genome shotgun sequence.
ACCESSION   DAEPLF010000346 DAEPLF010000000
VERSION     DAEPLF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.348"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            27..101
                     /locus_tag="KR314_003564"
     tRNA            27..101
                     /locus_tag="KR314_003564"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KR314_003565"
     tRNA            116..200
                     /locus_tag="KR314_003565"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="KR314_003566"
     tRNA            210..293
                     /locus_tag="KR314_003566"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="KR314_003567"
     tRNA            317..391
                     /locus_tag="KR314_003567"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="KR314_003568"
     tRNA            416..492
                     /locus_tag="KR314_003568"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="KR314_003569"
     tRNA            502..577
                     /locus_tag="KR314_003569"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="KR314_003570"
     tRNA            586..661
                     /locus_tag="KR314_003570"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="KR314_003571"
     tRNA            664..752
                     /locus_tag="KR314_003571"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="KR314_003572"
     tRNA            756..831
                     /locus_tag="KR314_003572"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="KR314_003573"
     tRNA            838..911
                     /locus_tag="KR314_003573"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="KR314_003574"
     tRNA            926..1002
                     /locus_tag="KR314_003574"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="KR314_003575"
     tRNA            1020..1096
                     /locus_tag="KR314_003575"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="KR314_003576"
     tRNA            1105..1180
                     /locus_tag="KR314_003576"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="KR314_003577"
     tRNA            1188..1261
                     /locus_tag="KR314_003577"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="KR314_003578"
     tRNA            1269..1345
                     /locus_tag="KR314_003578"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="KR314_003579"
     tRNA            1358..1433
                     /locus_tag="KR314_003579"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="KR314_003580"
     CDS             1509..1922
                     /locus_tag="KR314_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329915.1"
                     /translation="MYIVIGNEIVDSEELKIIIDENSKFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLSSLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
BASE COUNT          612 a          397 c          493 g          560 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actaaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taagaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagatga aaatagtaaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttgtccagt ttatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttaaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctata aa
//
LOCUS       DAEPLF010000347         2060 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.349, whole genome shotgun sequence.
ACCESSION   DAEPLF010000347 DAEPLF010000000
VERSION     DAEPLF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.349"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            67..1380
                     /locus_tag="KR314_003581"
     CDS             67..1380
                     /locus_tag="KR314_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBH0329916.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEENIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            1398..1934
                     /locus_tag="KR314_003582"
     CDS             1398..1934
                     /locus_tag="KR314_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBH0329917.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENKDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
BASE COUNT          812 a          202 c          401 g          645 t
ORIGIN      
        1 taaaaaagca taataatatt gtattgtaag gttttataaa cttctaattg aaaggggata
       61 attcaaatgg ataagagata tttagaatcc gatgtatttg aagcttttca agaaagactt
      121 tcttatcttt ttaatgaatt tgattatatc tatgtatctt ttagtggagg aaaagatagt
      181 ggcttactgc taaatttagt tttggattat aagagaaaga ataatataag ttccaaaata
      241 ggagtatttc atcaagattt tgaggctcaa tatagtttga ctactgaata tgttgaaaaa
      301 gtatttgaag agaatattga agatattgaa ccatattggg tgtgtttacc aatggctaca
      361 agaactgctg taggaaatta tgaaatgtat tggtatccat gggatgatga gaaagaagaa
      421 ttatgggtaa gagaaatgcc agtcaaagaa tacataataa atcaaaaaaa taatcctttt
      481 acttattata agtataaaat gagacaagaa agtttggcaa agcaatttgg tagatggatt
      541 agagatatac atgaaggtgc aaaagttgtt tgcttattag gaacacgagc agcagaatct
      601 ctacaacgtt attgttctat tgtgaataaa cagcatggat ataaaggaaa gtgctggata
      661 actaaggaat ttaaaaatgt ctggacggca tctcctatat atgattggga aatagaagat
      721 atatggttag cccactataa atttgattat gactataata aaatttatga cttattctat
      781 aaagctggag tagctccaag aaaaatgaga gtggcatcac cttttaatga ttcagcaaaa
      841 gagagtttaa atttatacag aatattggac ccagatgtat gggtgaaatt ggttgctaga
      901 gtacaaggag ttaattttac ttctatttat ggaaaaacaa aagctatggg atatcgtagt
      961 gttaaattgc catatggaca cacatggaaa agtttcacat attttttgct tgatacctta
     1021 cctgaaaata taaggaatag ctatattaaa aaattttcaa catctattga gttttggcag
     1081 aatacaggtg gtggtttgtc agaaatagtt atacgtgaat tgttgagaaa aggttataaa
     1141 atagaattaa atggagtttc aaatcataca aaagataaaa agagtagagt catatttttg
     1201 gatgatatac ctgatgatac ggatgatatt aaatttacca aagatatacc aagttggaaa
     1261 agaatgtgtt attgtatttt aaaaaatgac catatgtgta gatttatggg atttggtcct
     1321 acaaaagaac agagaattag aacggaatat attaagaaga aatatgaaaa aattcattag
     1381 ataggagtta agatgctatg gaatataaaa gtcctgtata tggaatcatc gcagtgccaa
     1441 tcgaaaagat agaatctaac gactataatc caaatgcagt agccccacca gaaatgcagt
     1501 tgttgtataa aagtataaaa gaagatgggt atacaatgcc aattgtctgt tattataatg
     1561 aggaaaagga taagtatatt attgtagatg gttttcatag atatagagta atgcttgaaa
     1621 ataaggatat atatgataga gaaaacggac ttcttcctgt ttcagtaatt gataaatctc
     1681 ttgatgaaag aatggcaagt actattcgac acaatcgtgc aagaggttct cataatgtgg
     1741 atttaatgag taaaattata actgaacttt gtgaaattgg aaaatctgat gaatggatat
     1801 caaagcacct gggtatggaa gttgatgaaa ttcttagatt gaagcaaata actggattag
     1861 cttatttatt taaagataaa gagtttggta atgcatggat tccctttggc tctgatatag
     1921 aagaagaaaa ttaaagagat ttattaaaag aaaaagatat attaaaaaga gaaaatttaa
     1981 aatatatatt gaaaataaag agctttaata ttatatataa gcacagaaaa aagagtagga
     2041 aaaatatcct attctttttt
//
LOCUS       DAEPLF010000348         2026 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.350, whole genome shotgun sequence.
ACCESSION   DAEPLF010000348 DAEPLF010000000
VERSION     DAEPLF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.350"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            97..1350
                     /locus_tag="KR314_003583"
     CDS             97..1350
                     /locus_tag="KR314_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBH0329918.1"
                     /translation="MLNKKNISSIIMTMILSLSTTVFVFADENTNVKGQDVTKYNEEM
                     VSKNLEDLSIEQLIDIRDKAIDKNLEGLRKFGEDSVKERKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVEYENSINDIMDPSGDFFKVTNQSYQINEFVLKNGKNYKEWSY
                     EYVEFLIEHLEDLCNFVDFDAKYVIEMVDPSIKPDLSDNQKKSLNNELIKMYSSNTLS
                     TNIKDEIENWENNLNINEKNSNEPLPRVSPSSYRQQALAYAKKHGYTNGYYGSTNRKN
                     NAPSPYYNFQKDGYGDCANFVSQCLHEAGVTFWTNNNPWYYYSTSNRSPSWAGAREFK
                     IHWMNRIAYQTLTVSNSLSFLRPATPVSILNSSGVATHTLISTDKHSNGYNFSYAAHS
                     DEGARTNLLKKLEGKKISYYKVYWE"
     gene            1684..>2026
                     /locus_tag="KR314_003584"
     CDS             1684..>2026
                     /locus_tag="KR314_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329919.1"
                     /translation="MLNKKNVSLIVLVIVLSLFTAIFVFNNRNTNDKSKEIAKNNEEI
                     VPKNLEALSIEQLTKIRDKAIDKNLEGLREFGEDSVKQRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQ"
BASE COUNT          865 a          191 c          297 g          673 t
ORIGIN      
        1 tagaaaaaaa cggtgataaa cttgttttaa attttagtag aatatgattt ttgttttggc
       61 aagtattaat acatatatta aagagggagg tttaatatgt taaataaaaa aaatataagt
      121 tcaattatta tgacaatgat tttaagctta tcaactactg tatttgtttt cgctgatgaa
      181 aatactaatg tgaaaggaca agatgtaacc aaatataatg aagaaatggt ctctaaaaac
      241 ttagaagatt taagtataga acaattaata gatattagag ataaagctat tgataaaaat
      301 ttagaaggtc ttagaaaatt tggagaagat tcagtgaaag aacgtaaaga ttcaatttta
      361 aactttgaat ttggattagt agcttttgtt aacaaaaatt atagtaactt aacagaaaaa
      421 gagaaattaa taagacaaat caatattgtt gagtatgaaa attctataaa tgatataatg
      481 gatccttcag gcgatttttt caaagtaaca aaccaaagtt atcagattaa tgaatttgta
      541 ttaaaaaatg gaaaaaacta taaagagtgg tcgtatgaat atgtagagtt tttgattgag
      601 catttagagg atttatgtaa ttttgtagat tttgatgcaa aatatgtaat agaaatggtt
      661 gatccttcaa taaaaccaga tctttcagat aatcaaaaga aatctctaaa taatgaatta
      721 ataaaaatgt attcaagtaa tactctaagt acaaatataa aagatgaaat agaaaactgg
      781 gaaaataatt taaatattaa tgagaaaaat tcaaatgaac ctttaccaag agtaagccct
      841 tctagttata gacaacaagc attagcttat gctaaaaaac atggctatac taatggttat
      901 tatggtagta caaatagaaa aaataatgca ccttctccat attataattt tcaaaaagat
      961 ggatatggag attgtgctaa ctttgtttca caatgcttac atgaagctgg agtaacattc
     1021 tggacgaata ataatccatg gtattattat tctactagta atagatcacc ttcttgggct
     1081 ggagcaagag aatttaaaat acattggatg aatcgaattg catatcaaac acttactgta
     1141 tcaaactctt taagtttttt aagaccagca acacctgtaa gtatattaaa tagtagtgga
     1201 gtagcaactc atacactaat atctacagat aagcattcaa atggatataa tttttcatat
     1261 gctgcacatt cagatgaagg tgcaagaaca aatttgttaa aaaaacttga aggaaagaaa
     1321 atttcttatt acaaagttta ttgggaataa ataaaaattt atacttttaa ttaaatgaaa
     1381 attgaaaaga ataaatatct atttcaagat ccaataagta tttagttgaa gcagctatta
     1441 aattttatta cataatcata aaaaagagac tagtggaatt tagtctcttt tttatgattt
     1501 aaaaatttaa taaaagaatt atataaattt gtctaaaaat tagtttatca ttcgttataa
     1561 ttatataaac tatatcaaaa aagtgttcta ttgtattctt tagaataatc agtaataaag
     1621 gttattctaa taaactgagt tattttttca caaataaaat acttattaaa aaggagattt
     1681 gatatgttaa acaaaaaaaa tgtaagttta attgttttag taatagtttt aagtttattc
     1741 actgctatat ttgttttcaa taatagaaat acaaatgata aatcaaaaga aattgctaaa
     1801 aataatgaag aaatagtgcc taaaaactta gaagctttaa gtatagaaca attaacaaaa
     1861 attagagata aggctattga taaaaattta gaaggtctta gagaatttgg agaagattca
     1921 gtaaaacagc gtaaagattc aattttaaat tttgaatttg gattagttgc ttttgttaac
     1981 aaaaattata gtaacttaac agaaaaagag aaattaataa gacaaa
//
LOCUS       DAEPLF010000349         2022 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.351, whole genome shotgun sequence.
ACCESSION   DAEPLF010000349 DAEPLF010000000
VERSION     DAEPLF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.351"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            178..294
                     /gene="rrf"
                     /locus_tag="KR314_003585"
     rRNA            178..294
                     /gene="rrf"
                     /locus_tag="KR314_003585"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            301..375
                     /locus_tag="KR314_003586"
     tRNA            301..375
                     /locus_tag="KR314_003586"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:333..335,aa:Asn,seq:gtt)
     gene            380..454
                     /locus_tag="KR314_003587"
     tRNA            380..454
                     /locus_tag="KR314_003587"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:413..415,aa:Glu,seq:ttc)
     gene            460..535
                     /locus_tag="KR314_003588"
     tRNA            460..535
                     /locus_tag="KR314_003588"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:493..495,aa:Val,seq:tac)
     gene            541..617
                     /locus_tag="KR314_003589"
     tRNA            541..617
                     /locus_tag="KR314_003589"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:575..577,aa:Asp,seq:gtc)
     gene            628..702
                     /locus_tag="KR314_003590"
     tRNA            628..702
                     /locus_tag="KR314_003590"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:660..662,aa:Thr,seq:tgt)
     gene            717..801
                     /locus_tag="KR314_003591"
     tRNA            717..801
                     /locus_tag="KR314_003591"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:751..753,aa:Tyr,seq:gta)
     gene            811..884
                     /locus_tag="KR314_003592"
     tRNA            811..884
                     /locus_tag="KR314_003592"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:843..845,aa:Gly,seq:tcc)
     gene            895..971
                     /locus_tag="KR314_003593"
     tRNA            895..971
                     /locus_tag="KR314_003593"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:929..931,aa:Arg,seq:tct)
     gene            981..1056
                     /locus_tag="KR314_003594"
     tRNA            981..1056
                     /locus_tag="KR314_003594"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1014..1016,aa:Gln,seq:ttg)
     gene            1068..1143
                     /locus_tag="KR314_003595"
     tRNA            1068..1143
                     /locus_tag="KR314_003595"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1101..1103,aa:Lys,seq:ttt)
     gene            1146..1234
                     /locus_tag="KR314_003596"
     tRNA            1146..1234
                     /locus_tag="KR314_003596"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1180..1182,aa:Ser,seq:tga)
     gene            1252..1342
                     /locus_tag="KR314_003597"
     tRNA            1252..1342
                     /locus_tag="KR314_003597"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1286..1288,aa:Ser,seq:gct)
     gene            1351..1427
                     /locus_tag="KR314_003598"
     tRNA            1351..1427
                     /locus_tag="KR314_003598"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1385..1387,aa:Pro,seq:tgg)
     gene            1513..1588
                     /locus_tag="KR314_003599"
     tRNA            1513..1588
                     /locus_tag="KR314_003599"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1546..1548,aa:Trp,seq:cca)
     gene            1649..1725
                     /locus_tag="KR314_003600"
     tRNA            1649..1725
                     /locus_tag="KR314_003600"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1683..1685,aa:Pro,seq:tgg)
     gene            1732..1808
                     /locus_tag="KR314_003601"
     tRNA            1732..1808
                     /locus_tag="KR314_003601"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1766..1768,aa:Ile,seq:gat)
     gene            1812..1887
                     /locus_tag="KR314_003602"
     tRNA            1812..1887
                     /locus_tag="KR314_003602"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1845..1847,aa:Phe,seq:gaa)
     gene            1895..1971
                     /locus_tag="KR314_003603"
     tRNA            1895..1971
                     /locus_tag="KR314_003603"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1929..1931,aa:Met,seq:cat)
BASE COUNT          520 a          451 c          540 g          511 t
ORIGIN      
        1 aatgtattta gcttatcgat actaataggt cgaggacttg accaatatta tttgatgtta
       61 ttcattaaga tatatgtgca gtttttagag tgttaactct aaaaaaaaat aaaaaaagta
      121 ttgacaaaaa tagtaaaaaa tgttaatatt atacttgtcc tagcaacaag aaaaaaatgt
      181 ggttataata gcagagaaga tacacctgtt tccattccga acacagaagt taagttctct
      241 agcgctgatg gtacttggtg ggaaactgcc tgggagagta gggcatagcc acgtgaatgt
      301 tccaaggtag ctcaacggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc
      361 ccacccttgg agccattatg gctcgttggt caagaggtta agacaccgcc ctttcacggc
      421 ggtaacaggg gttcaattcc cctacgagtc accatttgtg ggatcatagc tcagctggga
      481 gagcacctgc cttacaagca gggggtcaca ggttcgagcc ctgttggtcc caccatttgc
      541 ggcctggtag ttcagttggt tagaatgcca gcctgtcacg ctggaggtcg agggttcgag
      601 ccccttccag gtcgccaaaa taaaaatgct ggtgtggctc aacggtagag cagctgactt
      661 gtaatcagca ggttgtaggt tcgattccta tcaccagctc catgtaaaac taatacggag
      721 gatttcccga gcggccaaag ggggcagact gtaaatctgt tgtcatcgac ttcggtggtt
      781 cgaatccacc atcctccacc aattaaatat gcgggtgtag ctcaatggta gagttctggc
      841 cttccaagcc agctgtgagg gttcgatccc cttcacccgc tccaaagaaa gcgtgggtgc
      901 atagctcagc tggatagagc aacgcccttc taaggcgtgt gtccggggtt cgaatccctg
      961 tgcgctcacc acatatatat tggggattag ccaagtcggt aaggcacatg actttgactc
     1021 atgtatgcgt aggttcgagt cctgcatccc cagccaaata aaaatatggt ctattagctc
     1081 agtcggtaga gcacctgact tttaatcagg gtgtcccgcg ttcgagtcgc ggatagatca
     1141 ccaatggaga ggtgtccgag tggtttaagg agctggtctt gaaaaccagt gacttcgaaa
     1201 ggggccgtgg gttcgaatcc caccctctcc gccaaaaaaa tgactgaatt tggagaagta
     1261 ctcaagtggc tcaagaggat cccctgctaa gggattaggc tgggtaaccg gtgcgagggt
     1321 tcgaatccct ccttctccgc cattcaaaaa cgaggtgtag cgcagtttgg tagcgcacat
     1381 ggtttgggac catggggccg ggggttcgag tcccttcacc tcgaccaact taagctttta
     1441 gttcagttgg ttagagtgcc caactcatgg ctagtaattc tagggtttga gtccatgaag
     1501 gttattcttc ataggggtgt agctcagttg gtagagcgtc ggtctccaaa accgtgcgcc
     1561 gggggttcga gtccctccac ccctgccaaa taaaaaggac ttgaaaaaag ttggatttta
     1621 gagtagtata agatagtaaa taagatttcg aggtgtagcg cagtttggta gcgcacatgg
     1681 tttgggacca tggggccggg ggttcgagtc ccttcacctc gaccaagaaa agggcctata
     1741 gctcaggtgg ttagagcgca cgcctgataa gcgtgaggtc gctggttcga gtccagctag
     1801 gcccaccatt tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg
     1861 gtggttcgat tccgcctctg ggcaccagga atacggcggt atagcttagt tggctagagc
     1921 gttcggttca tacccgaaag gtcataggtt cgactcctat taccgctacc atctaactat
     1981 atcaataaag aactttgaaa attaaacagt aggttaattt at
//
LOCUS       DAEPLF010000350         2005 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.352, whole genome shotgun sequence.
ACCESSION   DAEPLF010000350 DAEPLF010000000
VERSION     DAEPLF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.352"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            76..1959
                     /locus_tag="KR314_003604"
     CDS             76..1959
                     /locus_tag="KR314_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0329920.1"
                     /translation="MVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTE
                     SYEGTINYNKDPEYLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIE
                     DITSRKDVNKYLRKTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGI
                     ISTPLATTYNAPILLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTV
                     NASQTRLKGSNRYETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPI
                     ILTDKNSITDNTYKWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGAD
                     RHETNANVIKKFYTDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYH
                     KDNLEAKSANKVYKIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGS
                     DTGTTGKPLGGIKEKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSL
                     RPDLFTSIHYNASDTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKT
                     RTLSTDPTKDYLYVLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIED
                     SLK"
BASE COUNT          856 a          260 c          347 g          542 t
ORIGIN      
        1 acaatggcat ttgctactga atctaatgct atggaaaata acgctgattt aaatataaac
       61 ttagagaaaa aaagtatcgt tttaggtagc aaatcaaaag tcagtgtcaa gtttaaagaa
      121 aaaccagatg cagatagcat tacattaaag tataaatgct atgatatgcc attggataca
      181 actctaaatt ataatcaatc aactgagtca tatgaaggaa ctatcaatta taataaagac
      241 ccagaatatt taaatgtttg ggaactacaa gggataacaa taaacagcaa aaataatcct
      301 aaaactttaa acaaacaaga actagaaaag atgggattaa atttaaaaga ctataatgta
      361 acgcaggaat gtataattga agatataact tctagaaaag atgtaaataa atatttaaga
      421 aaaacttctg cacctattac agaacttaca ggaagtgata gatacgaaac agcagttaaa
      481 ataagtaaag aaggctggaa aaatggttca gataaggtag ttataataaa tggggatgta
      541 agtatagatg gcattatatc aactccacta gcaactacat ataatgctcc aatacttttg
      601 gttgaaaaaa ataatgtacc taatagtgta aaatcagaat taaagcgttt aaatcctaga
      661 gatgtaatta taattggaga tgagaatgct atttctaaaa ctactgctaa tcaaattaaa
      721 tcgactgtaa atgctagtca aacacgtcta aagggctcta atagatatga gacatcttta
      781 ttgatagcaa aggaaataga taaaaatcat gatgtggaaa aagtatacat aacaaatgct
      841 aatggcggag aagtggatgc acttactata gcagcaaaag caggtcaaga caagcaacca
      901 attatattaa ctgataaaaa tagtattaca gacaacacat ataaatggtt aaagagtgag
      961 gatttacaaa atgcttattt tataggtgga cctcaaatga tatcaacaaa tgttataaat
     1021 aaggtaaatg atataactaa agataatgtt actaataata gagtatatgg agcagataga
     1081 cacgaaacaa atgcaaacgt aataaaaaaa ttctatacag atgatgagtt agaggctgtt
     1141 ttagtagcta aatcagatgt acttgttgat gctttagcag caggtccatt ggctgcgaac
     1201 ttaaaatctc caatacttat aacaccaaag acgtatgtat ctgcatacca taaagataat
     1261 ttagaagcta aatcagctaa taaagtatat aaaataggag gaggattgac ttctaaggta
     1321 atgaactcta tagcatcatc attatctaaa cacaatacaa ctccaacaga accaggaaat
     1381 agtgggggta agacagttat gattgaccca ggacatggtg gttcggacac tggaacaaca
     1441 ggtaaaccat tagggggtat taaagaaaaa gactatacat taaatacttc acttgcgaca
     1501 actgaatatt tacgttcaaa gggattcaat gtaataatga caagagacac agataagact
     1561 ttatctcttg gaaatagaac tgctttatct aactctttga gaccagattt atttacgagt
     1621 atacactata atgcatctga tacaactggg aatggtgtag aagtatttta taagcttaaa
     1681 gataaagatg gagggactac taaaactgta gctacaaata tattaaatag aatcttagag
     1741 aaatttaatc taaaaaatag aggtgcaaaa acaagaacac tatcaactga ccctacaaaa
     1801 gattatttat atgttttaag aaataatgat atgccagctg tacttgtaga atgtgcattt
     1861 ttggataatg agaaagatat gagtttactt aatacatcaa ataaagtaaa agaaatgggt
     1921 acacaaatag gtaaaggcat agaagattca ttaaaatagt ctacaatgag agcatagaaa
     1981 aatttaaaat aaattaatct gcaat
//
LOCUS       DAEPLF010000351         1982 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.353, whole genome shotgun sequence.
ACCESSION   DAEPLF010000351 DAEPLF010000000
VERSION     DAEPLF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.353"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            167..1771
                     /locus_tag="KR314_003605"
     CDS             167..1771
                     /locus_tag="KR314_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0329921.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGAANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVLDNNREFIKKYINR
                     FKVGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT          846 a          210 c          330 g          596 t
ORIGIN      
        1 tttgtcttat ttacaatgaa tagtatttac ttcttggcaa acaataatta aaaataaaac
       61 ttttatatga ttaaactcta aagaaaaagt ttataaagaa gataatataa ttaagcagaa
      121 aataatgaaa aaaatttatt aaagtagaca acgtaaggag aattttatga aagaaaaaat
      181 ttattacaat ggaaatatca tcacaatgga agattcaata tgtggagatg ctattcttat
      241 aaaagataaa ataataaaaa agataggaac aaaagaagaa gtatttgctt tgaaaaataa
      301 agatacagaa attatagatt tacaaggtaa gacattgatg ccatctttta tagattcaca
      361 tagtcacttg atagcttttg ctactactct aaagctagtt cctttagaag atgcaaccag
      421 ttttaaagac atagtaaaaa aaatacagga tttcaaagag tcgaataata taaaaaaagg
      481 agactggata attggatttt catatgataa taatttttta gaagaaaata agcatccaga
      541 taagagtgta ctcgatagtg catcttcaga aaatccaata ctaatttcac atgcttctgg
      601 tcatatggga gctgcaaata cattggggtt aaaacagctt ggtgttacca atgaaacacg
      661 tgacccagaa ggtggacata taggtagagt agaaggcagt gaagaaccaa atggatattt
      721 agaagaaaat gcatttttta atgtagcatc taaaataaaa caaccatcat caaatgagat
      781 ttttaattca attgaaaaag cacagaacat atatctaagc tatggtatta caacagctca
      841 agaaggttta atggaagaaa atcaatttaa cattttaaaa gcaatggcaa atcaaaataa
      901 attaaaaatg gatgttgtag gatatgtaaa tttgaaaaaa agtaagtctg tattagataa
      961 taatagggag tttataaaaa aatatataaa tagatttaaa gtaggaggtt ataagatttt
     1021 cttagatggt tctccacaag gtaagacagc atggttgagt agaccatatg aaaattcaga
     1081 tgatggttat tgtggatatc ctatatacaa agatgaagaa gtagaaaagt ttatagatat
     1141 ttcactaaaa gaaaagatgc aactcttaac tcattgtaat ggagatgcag cagcagacca
     1201 gttgatagat gcgtttgaga aagttttaaa cttaaaagag caaagtagtg aaaataatat
     1261 aagaccagtt atgatacatg ctcaaacagt aagagcagac cagattgatg atatgaaagt
     1321 aataaatatg ataccttcat actttgtagc tcacacatat tattggggtg atattcatat
     1381 caaaaacctt ggagaagata gagcgtttaa gattagccca cttaaaacaa ctatagaaaa
     1441 gggattaatt tacactcttc atcaggatac accagtaata gcacctaata tgctggaaac
     1501 agtatggtgt gctgtaaata gaattacaaa aaaaggtata caaataggag agaatgaaaa
     1561 gataagccca ttagatgctt taaagggtgt tactataaat gcagcatatc aatattttga
     1621 agaagataaa aaaggttcaa ttaaagaagg taaattagct aatttgataa tattagatga
     1681 aaacccactt acaatcgacc ctatgaaaat aaaagatatt aaagtattgc agactataag
     1741 agaaggagaa gtattgtatt ctttgaagta gatgaaagtt agatatttta taatctataa
     1801 aaaatagttt tatataataa gttaggtaga attaaataag attacagtta aatcaacaat
     1861 tatatttttt attaaatttt aattattgta ttgaagattt ataagtataa ttttattgat
     1921 tctacctttt taaataactg tcgatttatt aaagttatct atgtactata ttaaacaatt
     1981 aa
//
LOCUS       DAEPLF010000352         1932 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.354, whole genome shotgun sequence.
ACCESSION   DAEPLF010000352 DAEPLF010000000
VERSION     DAEPLF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.354"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            214..858
                     /gene="uppS"
                     /locus_tag="KR314_003606"
     CDS             214..858
                     /gene="uppS"
                     /locus_tag="KR314_003606"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBH0329922.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            901..1806
                     /locus_tag="KR314_003607"
     CDS             901..1806
                     /locus_tag="KR314_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0329923.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQKVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT          795 a          210 c          341 g          586 t
ORIGIN      
        1 atcaccactt gaagaacaac taaccattcc taatgataag attatcgaaa aaaacaatat
       61 taaaattttt ttcatgataa acctccaagt tttttaaact ctgtcttaaa ttagtttact
      121 acataattat ataaaaataa atgtatttaa tgttacagtt ttgattaata atgtaatatt
      181 ttgataataa tacctattga gaggtgatta gaaatgagaa taccaaaaca tgtaggaatt
      241 ataccggatg gaaatagaag gtgggcaagt caaaatggta tgacaaaaga taaaggatat
      301 gaaagtggaa tagaccctgg attagagtct tatagattat gtagagattt aggctgtgaa
      361 gaggtgactt tttatggatt tacaactgat aatacaagac gtccatcagt gcagacaaaa
      421 gcttttacaa aagcatgtat tgatgctgca aatatattaa tagatgaagg tgctgcttta
      481 ttagttgtag gaaatacaaa atctccaatg tttccagaag aattattacc atatactaat
      541 agaattaaag ccaaagaagg tcaaataaaa gcaaatcttc tggtaaatta tgggtggtat
      601 tgggacttaa ataatatatc aggagtagaa aatgtaaata aaaataatat acaggattac
      661 atacagtcat ttgatgtatc cagactagat ttaataatca gatggggtgg aagaaggaga
      721 ttaagtggtt tccttcctgt acaatcaatt tatgcagatt tttatgttat agataattat
      781 tggccagatt ttaagagtga cgatataaag gacgcattta gttggtatga tacacaagat
      841 gtcactttag gtggataatt aaaaatattc tgatttaagg ttgtagagtg ttttaaatta
      901 ttgaaaaaaa aacttcttga tggtaaaata acactaataa tatgtaaaag tgtaaaaatt
      961 tataccaaga agggtgaaga aatgtcaaaa ggcaataata acaataattc aagaaacaaa
     1021 tctaaaaaga ccagtcatct gaatagaaaa aagcgaaaat taaataagaa aaagttagca
     1081 gtgttgattt gctttacagt tttattttta tttattgcat ttaaggctac tcaaggtgta
     1141 gtagctttgg taaaaagtat ggataaaagt aataaaacta gtcaacaaca aaaagttaac
     1201 tcagaacaat ttgaccttgg taatgaagag gaaaataaaa agaaaaaata tactgtattt
     1261 atagacccag gtcatggagg caatgataaa ggaactgaaa gcaagaccag taatagatat
     1321 gaaaaagacc taaatctaca aattgcaaaa aaattagcaa ataaattatc taaacaaaag
     1381 gatattcaag tagtagtatc cagaactgat gatacatata tatcattaaa ggatagagct
     1441 atcttagcaa ataatagttc tgctgatgtg cttgtttcta ttcatttaaa tgcagagaaa
     1501 aatggtaata cagcaacagg tatagaaact tggtatagga ataaagctac agatggttca
     1561 aaagaacttg cacagacagt acaaagtact atagtatcct atgttaaagt tagagatagg
     1621 ggaatagttg aaaataactt tgaagtactt agagaatcaa atatgcctgc tatattaata
     1681 gagtgtggtt ttttaacaac accttcagag gaacaaaaaa taataaatga aaaatatcaa
     1741 gaccaacttg cggaaggaat tgttcaaggt gtactttcat acctagattc taaagggaat
     1801 aagtaagtta actttacttt aatgtgagga taaatttaaa agtttaactg tgtaaattgt
     1861 attaacttta tttcgtaatg ttggaaacat tgtttgaatt tagaaactat gaaattagaa
     1921 attattttat ct
//
LOCUS       DAEPLF010000353         1929 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.355, whole genome shotgun sequence.
ACCESSION   DAEPLF010000353 DAEPLF010000000
VERSION     DAEPLF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.355"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            564..737
                     /locus_tag="KR314_003608"
     CDS             564..737
                     /locus_tag="KR314_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBH0329924.1"
                     /translation="MHLVQLLCQQINGSDIDPDSKFGPATYNQVKILQGRLGVTKDGV
                     VGKATWQAVGERY"
     gene            complement(822..1844)
                     /locus_tag="KR314_003609"
     CDS             complement(822..1844)
                     /locus_tag="KR314_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBH0329925.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTLLNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNSGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTKLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
BASE COUNT          728 a          332 c          250 g          619 t
ORIGIN      
        1 ttatttaaga aatataggga ttttagaaaa aacaggcgca gatcttaagg aagaagatga
       61 tattagcatg agagttgtaa attaatagat ttttatagta ttataattta ttaacaaatt
      121 aggagaagtt ttgaaattaa aaatctaaaa aaaatcttag tagtcacagg gagggtgact
      181 ctaatatcta ttagtttagt atttgcaaat actaataatg atgtaaatgt atgaaaaagt
      241 aatgaagagc aaacaacaaa ttcatttaca ataactggtt acgaatttat ttatgaaaat
      301 gtaccacaac ctataaaatc tgattatgaa aaaaatgtaa agaattaaat ataaatccat
      361 ctaaagatga tacaatcttt attaatgaaa aatttataga ttcatataat attaatatag
      421 atgattataa tattgaacca aaagcattta ttattagata taaaaaagat ttaaatcgtt
      481 ttgatgctac tggtcctata agttatagcg taaatataaa taccactgta gtaggatatg
      541 gacatgtaga aaaagggaat cctgtgcatt tagtacaact tttatgtcaa caaataaatg
      601 gttctgatat tgatccagat agtaaatttg gacctgctac atataatcaa gtaaaaatac
      661 ttcaaggaag acttggtgta acaaaagatg gtgttgttgg aaaagccact tggcaagctg
      721 tcggagagcg ttattagaaa agaggttgtc gcactaagaa atagggcgac aactttttta
      781 tatagaatat attaatcagt atcttagagt ctagaaatat tttacaataa attccatata
      841 actctaccat ctatatcctt aacattttga caaccagtca aaatcatagt ctcacataac
      901 tcattcctaa ccttctctat aaaaaactcc acaccatcaa gaccaccgcc aaaagaagca
      961 gtaacaaaag gtcttcccat caaaacagca tcagcaccca atccaagcat cttaactaca
     1021 tcaacaccag tcctaactcc accatcaaca agtattgtta tctttccctt tacagactta
     1081 gctatatctg gaagtacctc acaagtacca ggagtataat caagtactct tccaccatga
     1141 tttgaaacaa taatggcact agcaccagac tctacagcca tcaaagcatc atctacagtc
     1201 ataattcctt ttataataaa aggtaattta gtagattcaa ccaattctct aatctcatca
     1261 attgtcttag gagaaaaagg attttcttga aattgattat taataagtcc acaagcatca
     1321 aggtctacac caactgcaaa agcacctgct tcttcagaca atctaatttt ttctattatc
     1381 tttgaattat tccaaggctt tataaataca attccatttc cactattatc tttcaacaca
     1441 tctaaattat ccagtaaaaa agtatctaca ttagtatcgc caaccatagc ataaattcca
     1501 gaattagaac aacctctaac aacaggctct atgtattctt tttcggaaac tttaccaccc
     1561 atgttcaata gagtacctga tacaggtgca gcgaatatag gagaactcat ttttctacca
     1621 aataattcta tggatgtatc tggctttgaa acattatgta taactctcat atttatcttt
     1681 attttttcta gactttttct attttcaatg aaagacgaac cacttccttt accacccata
     1741 ccaggaacat ctccagcaca agctaatcca ttacaaattt tacatacttt acatgttcca
     1801 ttaaagttct ctctagctga ttttagtaag tcattgtaat tcatacaaaa tctctccttt
     1861 ttataatatt aaaattgagt attactatat tgaaaatatc ttaattgtaa atatagggat
     1921 gttattatg
//
LOCUS       DAEPLF010000354         1926 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.356, whole genome shotgun sequence.
ACCESSION   DAEPLF010000354 DAEPLF010000000
VERSION     DAEPLF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.356"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            117..1166
                     /locus_tag="KR314_003610"
     CDS             117..1166
                     /locus_tag="KR314_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0329926.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWVQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            complement(1316..1663)
                     /locus_tag="KR314_003611"
     CDS             complement(1316..1663)
                     /locus_tag="KR314_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBH0329927.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
BASE COUNT          728 a          246 c          290 g          662 t
ORIGIN      
        1 gaaaattatt attaagtttt tgtagaaata ttataagcat agatacattt ttgtaacaac
       61 tacaataaat ttttaatact atatactatt cgtatgtctt atgggaggta tttattatgt
      121 ttaaaaaaat agtcgcaata gcaagctcta tcattgtaag tacaatatgt ctaactggat
      181 gtgattttaa ctctaatgac aaaaaagatg aatcttcttc gtctaaaaat ctggataaag
      241 taactatagc agaagttact cactctgttt tttatgctcc acagtatgct gctattacga
      301 aaggattctt tgaagatgaa ggtataaaga ttgaccttat aaatacccaa ggtgcagata
      361 aaactatggc agctttgatt tcaggtgaag caaacgtagg tttaatgggt cctgaagcat
      421 ctatatatgt atataatcaa aacaaaaacg attatgcagt gaactttgct caattgacaa
      481 aaagagatgg tagttttcta gttgctagga aaaaaatgcc taatttctct tacaatgatt
      541 taaaaggaac agaaatatta ggtggtagaa aaggtggagt tccactaatg acttttgaat
      601 atgttctaaa agaaaaaggt cttactattg gagaaaatct aaaagcaggc aatgttaatg
      661 taagaactga tgtacaattt ggtgtaatgg ctggagcttt tgcaggtgga gagggtgact
      721 ttacaacagc atttgaacct acagcaacag caatggaaaa agaaggtagt ggatatatag
      781 ttacatctat aggaaaagat tctggagaaa taccttttac agcatactct actacgagat
      841 catacatgaa aaacaataaa gatttaattc aaagatttac aaatgctgtt tataaaggtc
      901 aattatgggt tcaacaagca tctagcaaag aagtagctaa ggctatgcaa cctttctttg
      961 aagatatatc tttagaagat ttaacaacag ttgtagatag atataaatca atagatgcat
     1021 ggtctcaaaa tcctgtatta aaggaggaag gtttgaagaa attgatgact gttatgaaag
     1081 aagctaaaga actagataag gaagcacctt ataaagatat cgtaaatact gagtttgcaa
     1141 acaatgctat aaaaaatatt aaataaaata tattagtttt agctagatat tttcttgttt
     1201 ttataatgaa aattaacaaa aatataaaaa gagcctttta aaagactctt tttatatttg
     1261 agattaaagt ttatataata aaatagatat taaagtacta actgtactta tatatctatc
     1321 ctctgtattc tgctccagca ttaatttttg taatttgaac ggctaccatt tcttcattgc
     1381 ttccattagt aatttcatgc cactctcctg ttggtacaaa tacagtatcg ccgtttttta
     1441 catcacattc atgctcacca agtttcattt taccttctcc ttttaggaac acaaatattt
     1501 gttcaccatt agggtgtcta tgagcattaa gaacttgatt aggtttaaaa taataaactt
     1561 ctgaagttat atcgttacca gcaaatactg gtatttttgg aactgctgta tcattatatt
     1621 gagctaagct tccgtctaat agattctttt tattttcatg catactagaa atacctcttt
     1681 tctatattat agatttacta ttattatacc aatataagaa aatagtattc tcactttaca
     1741 caataggaat aaaattcttt tatttttaaa tataagtaaa tactattttt atagttagaa
     1801 aaatataatc attataagat ataaaaaaat taacataata taataaattc taaaagtatt
     1861 tttagatatc taaactttaa tataaagaat atatgaatat tttttataaa aaattaaaat
     1921 tattta
//
LOCUS       DAEPLF010000355         1898 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.357, whole genome shotgun sequence.
ACCESSION   DAEPLF010000355 DAEPLF010000000
VERSION     DAEPLF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.357"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..730
                     /locus_tag="KR314_003612"
     CDS             <1..730
                     /locus_tag="KR314_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329928.1"
                     /translation="IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIK
                     LSQDANSDIESIRYMEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESI
                     NNLKESKKVLEYLIEINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMY
                     SKALYYFKNKSMFLANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYD
                     ENIVVWNEVKGSMDWLLKKMLEFS"
     gene            complement(779..1828)
                     /locus_tag="KR314_003613"
     CDS             complement(779..1828)
                     /locus_tag="KR314_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0329929.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENRVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
BASE COUNT          716 a          290 c          293 g          599 t
ORIGIN      
        1 tattgacttt ttaaatcaag atactatagc tatttctaat gtagaatttt gggtaaaaga
       61 gaccaattcc aaatcaaagt ctgtagattt agatttagaa aagaaaaaaa tactatataa
      121 aagatatatt aagctatcac aagatgcgaa tagtgatatt gaatcaatta gatatatgga
      181 ggaagctgta aatataggta aaagtatgat tactaaagaa gataaagaac ttggattttg
      241 gaaaggttta tctaccagag cctatatgat agcacttgaa tcccttgcta gaaagttaga
      301 aagtattaat aatctaaagg agtccaaaaa agtattagaa tatttaatag aaattaatcc
      361 tagtgatgag cagggaataa gatacaaatt attaaatgta ttaattagat taaatgatag
      421 aacttcaata aataaattat ttgaacttta taaagaagaa aaatctgcta catggatgta
      481 cagtaaagct ctttattact ttaaaaataa aagtatgttt ttggcaaatg attctattaa
      541 ggttgctaga agtaaaaata agtacgttgg attatatttg ttagattggc gaaatgcttt
      601 tggtagagaa tttgttacag aggaagaaaa ggcagaagct gtctattact atgatgaaaa
      661 catagtggtt tggaatgaag ttaaaggttc tatggattgg ctattaaaaa aaatgttgga
      721 attttcataa aaaaaactac tccttacttt tatataagta aaaggagtag tttttaattt
      781 atacatctag ttttaaatct tcaggtttta ttagaccttt ttttctcata aattctgaaa
      841 tcaacaaagt tacaatagca ggaagtataa aattaagtaa aagtatacca acccacatag
      901 atatacctcc ttttccagca ctttgcatag cagttactgt tccaaattgt ccaaccaatc
      961 cacaagtacc cataccagaa ccaataggaa tattctccat tttgaatatt gttgtagata
     1021 atggtccaag taatattgaa gctacagtag gtggtatcca aattttccaa ttttttatta
     1081 tattaggtac ttgcaacata gatgtcccaa gaccttgtgc cacaaggcca ccaactctat
     1141 tttctctaaa gctcataaca gcaaagccta ccatttggca acaacaacct acagttgctg
     1201 caccaccagc aagaccacct aaacttaaca tcatacaaat agctgcacta cttataggta
     1261 atgtaagagc tattccaaca agtgcagaga ctaaagctcc cattattata ggttgcatag
     1321 ttgtagcttg cattattata tttccaaatg aagtcataaa agcagaaaca gctggaccaa
     1381 taagttcagc tacaagtact ccaattagta ttgtcacagc aggtgtaacc aatatatcta
     1441 tttttgtttc ttttgaaact aattttccaa cctctacagc aaaaagtgta gcgacaaatg
     1501 ctccaacagg tcctcctaat tcattaccac aagctccaac aagtgcacaa gaaaatagta
     1561 caagtggagg tgcttgaagt ccatacgcaa tagccactgc tatagctgga ccagtaacct
     1621 gtttagcaag agggcttata acttcagtaa aaagagaaat attgaaatta tctcctaatg
     1681 tattgaaaat tgttccaatt aaaagggacg aaaaaagacc aagtgccata taacttagtg
     1741 cgtcaataag atacctttga acagaaattt caatattttt tcttttagca aatgatttga
     1801 aatttctttt tttataattg atttccaatt taaaatcctc ctcatataat ataagtataa
     1861 tttcttttta attaattata taacttttca gctgacaa
//
LOCUS       DAEPLF010000356         1898 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.358, whole genome shotgun sequence.
ACCESSION   DAEPLF010000356 DAEPLF010000000
VERSION     DAEPLF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.358"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            497..976
                     /locus_tag="KR314_003614"
     CDS             497..976
                     /locus_tag="KR314_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0329930.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRTLLKTKLENTEVII"
     gene            973..1752
                     /locus_tag="KR314_003615"
     CDS             973..1752
                     /locus_tag="KR314_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329931.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKALDKENNVLGKSKALSFEYKNGNDEISLDTANNVLEKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
BASE COUNT          789 a          237 c          255 g          617 t
ORIGIN      
        1 tttataattt atatttttta agtttattta tgttcatatc tatctaaaat aatgacattt
       61 ctaactcttg attctcattt aagtttcatt gttttttctt aaaagcctac ttttagtcaa
      121 aataccgtta tctttagtca ttaattaaaa aacttatttt aaatctatac atgaagtaaa
      181 taatccaaaa gcattatttt ctaactactc aacatcaaat acttactatt ttataattac
      241 ttaatataaa cacttactat aaaactaaat atataaatgg cttctaaagt ttaatatatc
      301 tacatggctt tatatatgag gggaaaatac tagaaaaaaa tatattatag aatatttgta
      361 tagaataggc aaaactttca aacttaaatt tatcaaataa tcataaaaaa agtgaaccat
      421 ctatagtaaa attttatcta ataagtgaaa ggcaaaaaag tagcgctacg atttgcatag
      481 aagggaagga actcagttgg ataaaactac atttacaaac aatatacttg aatcagaaca
      541 gactttgtat cgagtatcta agtctattct aggaaacgac caagactgtg aagatgccgt
      601 caacaatgct attttaaaag catatgaaaa gttggattct cttaaggaag aacaatactt
      661 taaaacatgg ctgattagaa tagtcataaa tgaatgtaat tcattaagac gcaaacgatt
      721 aaaatctcta tcatttgaag atgtcttcaa aaacaaaaaa attgatgaaa aagatgacta
      781 tagcgatttg tatactgcta ttcaaagtct acctaaaaaa attaaaattc caatagtcct
      841 ttactatatt gaaggttatt cagtagatga ggttaaggaa atattagata tacctcaagg
      901 aactgtaaaa agtagattat caagaggaag aacattatta aaaactaaat tagaaaacac
      961 ggaggtaatc atatgaataa aaatatatta aataattcgt ttccagaagt tccagaaagt
     1021 tttcataata gtttaagtaa aacactaaat agtttaccag aaagagagga aaattataaa
     1081 atgattaata ataaaacaca caaattacca tttagaaaag gcttagttgc aactttagcc
     1141 attactttga tattgagcac aacagccctt gcagtaggac atatatatag tttagttgga
     1201 aaatcaagta ctaaggctac ctatacatct atcccaacta cagaacaagt aaaaaaagac
     1261 tttaacttta cccctaatat agtttctgaa tttagtaatg gatataaatt taaaggagca
     1321 tatactgtaa ataaaaaagc acttgacaaa gaaaataatg ttttaggaaa atcaaaagca
     1381 cttagttttg aatataaaaa tggcaatgat gaaatatctc ttgacacagc aaataatgta
     1441 cttgagaaga gttctgacaa tctaaaagtt gtagatactt acaaagatat taatatttct
     1501 tatgaagcat tcgcacaaaa gtttgtacca gaaggttata aaatgacaga acaggataaa
     1561 attgatgaaa aatcaggcaa atacacattt agtgtagggc caaataatga atcagacaaa
     1621 gttgaagtta atgaatttaa gtttttgaca tggaaacaag atggagttta ctattctttt
     1681 acagttcaag actcaaattt atctatgaat gacttagtta aaatggctca tgaagtaata
     1741 gatgcaaaat aaacataagt aataataagt tgtttatgag tttattattt atgactttaa
     1801 tttttttatt agtaaacatg gttgtatctt ccatctttat attttcttta aatttaatat
     1861 ataaaatttt agatatttag ctaccttcaa aagaacca
//
LOCUS       DAEPLF010000357         1894 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.359, whole genome shotgun sequence.
ACCESSION   DAEPLF010000357 DAEPLF010000000
VERSION     DAEPLF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.359"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            156..725
                     /gene="mocA"
                     /locus_tag="KR314_003616"
     CDS             156..725
                     /gene="mocA"
                     /locus_tag="KR314_003616"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBH0329932.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            722..1741
                     /locus_tag="KR314_003617"
     CDS             722..1741
                     /locus_tag="KR314_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBH0329933.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT          760 a          166 c          361 g          607 t
ORIGIN      
        1 agagatattt tatggaagcg taaaacaaaa attttgtgta agatattgaa gtattaaaat
       61 agtttatatg atattatata tatagataat atcaatagag atattataaa aatcatagaa
      121 ctatcaatat attataaata ccaaggatga tatatatgat aaatgcagtg ataatggcat
      181 caggctttgc cagaagaatg ggagcaaata aacttttatt aaattataaa ggctgttcta
      241 taatagaaca tgtatttaga gaagttagca agattgattt tcatcaagtt gtagtagtaa
      301 gtcaatatga agaggttttg ttattgtgta ataaatatgg ttttgaatat atagataata
      361 aaaatgcaaa tataggtcaa agtgaatcta taaaattagg agttttaaat agctcaaaat
      421 gtgatggtta tatgttcttt gtaggagacc agccatttat tgataactta tatatagata
      481 aaattataaa tacttttaaa ttagatagag attttatagt aattccaaga tacaaagata
      541 tgtgtggaaa tccagtcata ttcccatata gtaagaaaga agaattgttg gcacttaaag
      601 aagatgaaaa aggtaaaacc attttaaaaa attcatcaaa aataaagtat gtagaagttc
      661 ctaaagaaat gttatttgat atcgatacaa aaatggacta tgagagaatc gggggtaaat
      721 tatgaagcta ataaaaacta tagatgctgt tggacaagtt ttatgtcacg acataactca
      781 aataatacct ggagaattta aaggaagaaa atttaaaaag ggtcacatag taaaagaaga
      841 agatattcca gtgttattat ctcttggtaa agataatttg tatgtatggg aaaagtcaga
      901 aggaatggtt catgaaaatg aaggcgcatt gtttttaaaa gaactaactg ctggagaaaa
      961 tttagatttt agtgaaataa aagaaggaaa aatagatttt atagcagcat gtgatggtct
     1021 attaaagata gatgttgatg cactttttga tttgaattgt gttggagaaa taatgatggc
     1081 tactcttcat aataatttta tagtaaataa aggtttaaaa gtagctggaa caagagtgat
     1141 acctttggtt atagatgaga aaaaattaga agatgcaaaa aaagtagttg gtaatagaaa
     1201 aatagtaaat gtagtacctt ttaaacctaa gaaagtagga attgttacta caggaaatga
     1261 agtgttttat agcagaattg ttgataaatt tggaccagta atagaagaaa aagtaaaagg
     1321 ctttggttgt gaagttatag gacagactat atgcccagat gataaagaaa ttataaagag
     1381 tgcaataaaa gaatttataa gtcaaggagc agaacttatc tgttgtactg gcgggatgag
     1441 tgtagaccca gatgatgtta cgccaacagc tataaaagaa actggagctg atttagtgac
     1501 atatggttca ccaatattac caggagctat gtttttactt gcttattatg gagaagttcc
     1561 aattatgggt ataccaggat gcgcaatgta tcataaaact acagtatttg atatagtttt
     1621 atctagggtt ttaattgatg aaaagcttga taagtatgat atagctagat atggtcatgg
     1681 tggcctttgt atgaactgtg atgtatgtac ttatcctgca tgtaattttg ctaaaatata
     1741 aagttattaa aaaagagtat ttacaaatta agtactcttt ttaaatatga aacaaaacag
     1801 ctagaatgat gttttaagaa aagcatattt atttagaaat tataataact tattttcaat
     1861 gtgtaaaaga atgaataaag ttgcaatttg attt
//
LOCUS       DAEPLF010000358         1828 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.360, whole genome shotgun sequence.
ACCESSION   DAEPLF010000358 DAEPLF010000000
VERSION     DAEPLF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.360"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            679..1386
                     /locus_tag="KR314_003618"
     CDS             679..1386
                     /locus_tag="KR314_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH0329934.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          777 a          151 c          294 g          606 t
ORIGIN      
        1 attagaattt ataaatattt attttagatt attgtaaata tttatttaaa aaaaatagaa
       61 ttattatatt tatgataata aatggtataa taaaaggtaa agtgtcaaat atgcactaaa
      121 ttaaataagt taattagtat ataggttaaa gtggaagtaa ttaaaactta ttaagtatta
      181 gaaaatgact tcttattaga gtaagtatct aaaatgatat aaaaattatg attactcatt
      241 aaagtgacta ttatagagta gataaaattg cccataaaaa tagctataat agagtggata
      301 agattactca ttaaagcaat tactatagag tagataaaat tactcactaa aataactata
      361 atagagtgga taagattatt cattaaagcg attactgtgg agtagataaa attactcact
      421 aaaataacta taatagagta gataagatac tcattaaggt gattattgta gagtagataa
      481 aattacccat caaagttaat tatcacatat tagataagat tattcattaa agttactatc
      541 acagagtgga taaaattaca cattaaaatt gtctgttaaa gaatgaataa aaaagagatt
      601 gctcaatata agaagcatat aaaaaatcaa acatataaaa tttaataaat aataactgat
      661 agggaaagga aagatagaat gatatttaca aaaagcaata aatacgataa ggactttctc
      721 atgaaaaata tgatgggacc taattgtata aaaatattgg aagaattaac aagtaagata
      781 aaactagaaa agggaatgcg tattttagac ttaggttgtg gtaagggtat atcttcgata
      841 tttttagcaa aggaatttga tgctactgta tttgcaacag atttatggat agaaccaact
      901 gaaaactata aaagatttaa agagtttaaa ttggatgata aaatatttcc aatacaggca
      961 gaggcacatg aactacctta tgcagaaggt ttctttgatg cggtgataag tatcgattct
     1021 tatcactatt ttggaaacaa agaaggattt ttagacaatc atatatcacc tcttgtaaaa
     1081 gaggggggaa tacttgctat ggcaatgcct ggattaaaag aagattttgt ggattgtata
     1141 ccagatgaac tcattccatt ttggcaagat aatatgaact tccattcaat tacatggtgg
     1201 aataaacttt ggtctgaatc ggagtctgtg attgtagaga aatgtgaagc tttaaattgt
     1261 catgatgaag cttggaagga ttggataaat tgtgataatt catatgctat aaatgacaaa
     1321 aaaatgatgg aagtagagaa tggaaagtat tttaatacaa tttctttgat agcgagaact
     1381 aaataattgt gatgaattta taaaagtata agataagtag aaatataagt tgaatgagag
     1441 tataagaagc atagaaatat aagttgaatt aaagtagaag gtaaataaaa atgtaagtta
     1501 aataaagagt ttatacaaaa atgatttttt agattattta tacccaatcg agttgcttag
     1561 gtcagttggg tatttttatt tttttaaagt taaattgcaa atactattgt gtgatatata
     1621 ttttaatact aaatgtttgt tttataagaa ataaactaat agaaaaaata gttttaacaa
     1681 tgaaagcaaa aaaggtattg atgaagatta aaaattttat agtaccattt tatatatttt
     1741 tttatatatt ttattatact ttaaatcaaa tttcaaaatg attaagtata tatacccaaa
     1801 agattaaaga actatattta ttatacta
//
LOCUS       DAEPLF010000359         1809 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.361, whole genome shotgun sequence.
ACCESSION   DAEPLF010000359 DAEPLF010000000
VERSION     DAEPLF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.361"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..353
                     /locus_tag="KR314_003619"
     CDS             <1..353
                     /locus_tag="KR314_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0329935.1"
                     /translation="YKLTNGLYFYLIYINKNRNCSLNDVSTEFEVDKAHTTRTISRLE
                     QDGYIEKIQNPNDSRAFQLRVTDKGEEVLGDIKNIFSKWDNHIKKEFSDTEYKELVKN
                     LHVVKDIKTAVEEG"
     gene            355..1449
                     /locus_tag="KR314_003620"
     CDS             355..1449
                     /locus_tag="KR314_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBH0329936.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVIKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1537..1653
                     /locus_tag="KR314_003621"
                     /pseudo
     CDS             1537..1653
                     /locus_tag="KR314_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
BASE COUNT          731 a          193 c          334 g          551 t
ORIGIN      
        1 catataaact tacgaatggt ctatatttct atttaattta tattaataaa aataggaatt
       61 gttctttgaa tgatgtttct acagaatttg aagttgataa agctcataca acaagaacta
      121 tatctaggtt agaacaagat ggatacatag aaaaaatcca aaaccctaat gatagtagag
      181 cttttcagtt gagagtaaca gataagggtg aggaagtatt aggggatata aaaaacatat
      241 tttcaaagtg ggataaccac ataaaaaaag agttctcaga tacagaatat aaagaactgg
      301 taaagaattt acatgtggta aaggatataa aaacagctgt ggaggaagga taggatgtat
      361 tgtaatttat taaaaccaat taaaattgga aatttggaat taaaaaatag agtatctttt
      421 gcacctacaa gcatgggtct taaattggaa gaaaaaatta aaaagttttc tgatattgct
      481 aagtcaggag tagctctgat tactctagga gatgtatcta ttagacctag ttttcataag
      541 gtagctataa gcctatcgga tgaagatgga gtcattaaat acaagaaaat tgtagatgaa
      601 atacacaatt ctggtgcaaa ggtatcagca caactatttt gttcagatta tgatgttaat
      661 ttaattaaag atactatgaa gatgggaatt acttcacatg atgagataaa aaaaataatg
      721 aatgacggag taaaagatta cataacaaat atgcccaaag aagaaattaa aaatattata
      781 aatttattta aagtaacagc attgaatgca aaaaaagcag gttttgacat gattcaaatt
      841 catggtgata ggcttgtagg aagtttttca tcaagtatat ttaataatag aaatgatgaa
      901 tatggtggaa cgtgtgataa tagaagtaga tttgcaagcg aaataattag tagtatccga
      961 gatgaagtta aggacattcc aatagactat aaatttgcaa taagacaaga aaatcctcat
     1021 tatggaaatg caggagtttt actttcggaa gttgagtatt ttgttaaaaa atttgaatcc
     1081 ctaggtgtaa acagttttca tgtaacactt gcaaatcatt caaaattaga agatactata
     1141 ccaaccaata atcatcctta ctttaaagat gaaggctgtt ttttatattt agctgatgaa
     1201 gtaaagaaac acacaaatct tccagtatgt ggggttggaa aattgtctag cccagatttt
     1261 atagaaagta ttatttcaaa taatagagta gatatggttt ctatgtcaag acaattacta
     1321 gcagatagta attggttaca gaaagtaaaa gatggaagag tagatgagat taaaaagtgc
     1381 tgttattgta ataagaagtg tgcagatgct ttacaaacac gctctcagtt tggatgtata
     1441 ctagattaga atatctttat aaaaataaat aatttgttaa ggttaatata tttttggtga
     1501 tttagataag tatttagata aagaggagga gacatcatga aaagtttagt tttatattca
     1561 agtttaactg gaaatactaa aaagattgct tatgctatat atgatgaaat tcaagaagaa
     1621 aaagacatta aagatgtaaa tgaattagtt gattaaaaaa tagctacgca cacttgtgac
     1681 tttagtcatg agttagtagc taaaatagtc agcatatagg gaaacctata tgtagagata
     1741 ggatagaacc tatccaatac tacttgaatt gctggaaacc cctaaagcta atcaaactaa
     1801 aacataagg
//
LOCUS       DAEPLF010000360         1766 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.362, whole genome shotgun sequence.
ACCESSION   DAEPLF010000360 DAEPLF010000000
VERSION     DAEPLF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.362"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(93..1013)
                     /locus_tag="KR314_003622"
                     /pseudo
     CDS             complement(93..1013)
                     /locus_tag="KR314_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
     gene            complement(1302..>1766)
                     /locus_tag="KR314_003623"
     CDS             complement(1302..>1766)
                     /locus_tag="KR314_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0329937.1"
                     /translation="ELSRKTIGSLNRNKARLKVARLQEHIANQRKDFLQKLSTKLIKE
                     NDIICIEDLQVKNMIKNHKLSRSISDVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQ
                     ICNKCGYKNEEVKDLSIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT          560 a          265 c          214 g          727 t
ORIGIN      
        1 taacaaataa taaaaaaatt aatttgattg tatataaatt agtttgttat taaattagta
       61 ttaaaaagtt caaaaattta cttagaaaga atctataatt cgatttctat gtttgttgag
      121 aatgcaatta cagcttttcc aattattttt acttcaactg gcttattatc tgaaataatt
      181 acttttacta acatcttgcc tttgcgtttt attgcttctc cttgaactgc ttcaaatata
      241 aactctgtgt tgggtatagg tattacttta taatgtacaa ggtacgcacc taaaggtcca
      301 ttagcatttc ctgtaacagg gtcttctgaa ataccaattg caggagcaaa cattcttcca
      361 tgaacttgta gagtttcttt attatgtaat gtaaaaatat aatatccatt acattttatt
      421 tcttgactta ttttcgataa ttcatataaa tttggagata aactatttaa tgttgataat
      481 tcctttattc caatcataac ttttgaatgt cctgtagatg atatggcaat aggacaatct
      541 gtacgaatat cttcatttaa aataccaaat gcattaagta atcttttttg ataggtttgt
      601 tgtaatggtg gagatacttc aatttttcct tgtgtcataa ttatttcata atcattatct
      661 ttttttataa tatcaactgg taatattcca gcaccagttt tttgaaatac tgttttgtta
      721 ttgaattggt tttctactgc tctaacataa tgggcagcaa tagtagcatg accacatata
      781 ggaacttcat tagttggtgt aaaaaatctg atatgtacat cataattttc ttgatgattt
      841 ttagccaatt tattgaaaat aaaagcagtt tatgaattat ttaattctct tgttattttt
      901 tgcatttgtg attcagataa gccatcagca tttgtaatta caccagctgg attacctgtg
      961 aatctttctt ttgtaaatga gtcaacttga taaagacgaa attttttatt catattatat
     1021 aatcctttct aaacattaga ttagtttatc tacatagaaa acttgagata aattattatt
     1081 tattattatc tgtttaaata aaattaataa aataacatat gtctatatta aaatttaaaa
     1141 atatacatct aaaaaagtag tattatgtaa ggctctatgc tacactgaac ctcccatgac
     1201 taaagtcaca gggttcctgc ttcatagaat tttgcatact aaaatatgtc ttactaattc
     1261 tccacaggct atccccgtag ttcctacggt tcttacatac gttatttatt ttgaattgtt
     1321 attagtctta atccttcttt taatatattt atacttgcat ttatatctct atcgtgagtt
     1381 tcattacaac taggacaaat ccattctctt atacttaagt ccttcacttc ctcgttttta
     1441 tacccacatt tattgcatat ttgtgaactt gcaaagaatt tacctacctt tacaattttt
     1501 ctaccatacc aattagcctt gtactctaat tgacgaataa attctgacca tgatacatct
     1561 gaaattgagc gagatagttt atgattttta atcatattct ttacttgtaa atcttctatg
     1621 caaataatat cattctcttt tattaattta gtagatagtt tttgtagaaa atcctttctt
     1681 tgattagcta tatgttcttg aagtcttgca acttttaatc ttgctttgtt tctatttaaa
     1741 ctaccgattg tttttctcga taattc
//
LOCUS       DAEPLF010000361         1762 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.363, whole genome shotgun sequence.
ACCESSION   DAEPLF010000361 DAEPLF010000000
VERSION     DAEPLF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.363"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(37..300)
                     /locus_tag="KR314_003624"
     CDS             complement(37..300)
                     /locus_tag="KR314_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329938.1"
                     /translation="MIKNTKQITVEVNEILLQEISKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            616..1191
                     /locus_tag="KR314_003625"
     CDS             616..1191
                     /locus_tag="KR314_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329939.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            1289..>1762
                     /locus_tag="KR314_003626"
     CDS             1289..>1762
                     /locus_tag="KR314_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 family protein"
                     /protein_id="HBH0329940.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNI"
BASE COUNT          695 a          166 c          280 g          621 t
ORIGIN      
        1 aaaaagttcc tagtttttag gaactttttt atttgattaa tacagggtat gagtttttgc
       61 ttcagctata gctcttgctg cacttttagg tttttcagta ttttcttttt tgtaaaattt
      121 aatcacttct tctaaatctt tattttcaat gttatgtttt ttgttttcaa gaatataatt
      181 tacaattgat tcatttatca aatctgtcaa aatattattt tcgtcattag ttatcttaga
      241 tatttcttgt aataaaattt cattaacttc tactgtaatt tgttttgtat tttttatcat
      301 atcaaccacc tcttaagaaa attatatcat ctcaaaaaaa taaattcaat atgttttaaa
      361 attatagaat tttaattctt aaattctaaa aaagagaaaa ttaaataaag tagctttaga
      421 ttttaaaaga ttaataaaaa atatttaacg ataaaagctt tattctatat gattctaaga
      481 tagaggattt ataagtgttt aaggtgaatt aaatataaga caacttctta aagcaaatgg
      541 gtgtggtatt taaatctaaa tatagacata taatattaaa tatgctaata tatcaaaata
      601 ataagggggg aatttatgaa gcttaaagaa gcattgatta catgtgttgt atgtagtgta
      661 tgtttaactc cattattggt aaatggagaa acaaaaatag atgcagatca ttgtaaagaa
      721 tctatagtaa atatagatac tatagtaaag tcaggtaaaa gctttaattc caagtataat
      781 caaggtatgg aagattataa aaatagtgat gagtataagt tgaaagtaca aaaggagaaa
      841 ctacaaaatc agttaggtat taaaataaag aagttagtac ctataacttt tgaagttagt
      901 tattacacaa gtcttaatca agaaaatgga tatggagcga taactgctac aggagatgat
      961 ttagaagatg gttttgtagc aaacaatcac ttgagttttg gaactaagat tattgtagat
     1021 ggaaaattaa aaactgttat ggataggggg tctagtaagt attttagtaa ttctaatgca
     1081 atagatgtat ttgtgcccaa atggagtaat gaaagtgaat cagagtacta tgatagagtc
     1141 aataatatgg gaagacatta caaaaaaggc tacattattg ttgaagaatg atgttctttg
     1201 aagtaatatg tattgattgt agtatttaat atatatataa taaatataaa actattagta
     1261 caatgagata tattactagg agggcaatat ggaacaatcg tttataaatg gaaaagtaaa
     1321 cttattaata gaaaattaca aagcattaaa tgaagttaaa ggttcttggc aaatgggatt
     1381 gatacaacat agttgtgcac ttgcatttac tcttaagaac aagagaataa gtcctagatt
     1441 agtggaagaa cgaatagagc ttattaaaaa aaatactgga ttattctcta attttagagg
     1501 atataatatg ttttatatgg caacactact ttcgtttgaa tcaaatccag aaagtagttt
     1561 taagatgatt ttagatatat acaaagaact taaaagtgaa aagttttggg gcgatacata
     1621 tttaccactt acagcatcaa tagtttatga aaatagagag aaaatggatt atcttacatg
     1681 tatatcaaaa atgaaaataa tatatgatta catgaggaaa aaacatccat ttctaacttc
     1741 tagtgatgac tattgtaata ta
//
LOCUS       DAEPLF010000362         1752 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.364, whole genome shotgun sequence.
ACCESSION   DAEPLF010000362 DAEPLF010000000
VERSION     DAEPLF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.364"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..539)
                     /locus_tag="KR314_003627"
     CDS             complement(<1..539)
                     /locus_tag="KR314_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329941.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIIN"
     gene            730..1692
                     /locus_tag="KR314_003628"
     CDS             730..1692
                     /locus_tag="KR314_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBH0329942.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
BASE COUNT          630 a          210 c          319 g          593 t
ORIGIN      
        1 taatttatta ttactgcttt caatgttttt tctccttgat tatggtatgt atgaattgat
       61 tcagctgaaa aacaaataga gtcattttct ctaagttgat aaatagagtc atttacttct
      121 aattttaatt gaccttctat aatcataata tattcctgtg atttttcaga atgaccaaca
      181 gatgtataac tatgaccttc ttctaattcc atttgaaata attcgaagtt tctagttggt
      241 gtattgggat aataacaata aagacgataa tctttatttt ctgaaacttg tacatctata
      301 tcagtttttg atacaataaa agtttcattt tgaggttgtt ccagtattgc agtatatgga
      361 acatttagtc cacttgcaat tttccaaatt gtgtttactg ttgggtttga gtctcccttt
      421 tcaatttgcg aaagcattac tttacttacg ccagataatt cagcaagttg acctagactt
      481 aagttacgtt cattacgtaa tctatttaaa tttttagcaa taacagtatt aatactcata
      541 gtaactcctt aaaatgtaaa ttattgacaa atataattta cgaatgtata atttatttaa
      601 cattatttat aatttacaaa tgtataatat aatgaacgtt aaatacactg tacatccgta
      661 atataaatta tactgcataa agagttgttt ttcaacggat gatagttgag agattaaggg
      721 ggaattgtaa tgcgtgtact taataatgtt acagaggcta taggcaatac tcatatgtta
      781 catttatcca aaatagcaga tgaatttgga gttcaaggta atatttatgc aaagatggaa
      841 tacttaaatc cgggatttag taaaaaggac cgtgtagcat tacaaatgat taccgaagca
      901 gaaaatgcag gtttattagt tccaggtcaa gcagttgtgg aattaacaag tggtaatact
      961 ggaacaggac tagcaatagc ttgtgcatgt aaaggatata aatttatagc gtgtatgtca
     1021 aaaggaaatt ctatggaaag agcaagaatg atgaaagctt taggtgctga agtagttctt
     1081 gtagaccaaa tgcctaattc agttcacgga caggtatctg gtgaagattt ggcacttgta
     1141 gaaattgagg ctcaaaaaat agcaaaagaa agaaatgcat ttagagcaga ccaatttaat
     1201 ttagaagctt gtaatcatgc acatgaaaag tatacagcag aagaaatgtg ggaacaattg
     1261 gatggaaata ttgatgtatt tgtagatttt cttggaagtg gtagtacttt tgcaggatgt
     1321 tcaaaaacac ttaaaaaaca taattcttct atcaaatgtt atgttgttga acctgaaaca
     1381 gcagcaattt attcaggtaa agaaataact gaccaagggc ataaaataca aggtggagga
     1441 tattctatgg atttgccatt agtagacaag aaacttattg atggttatat tcaagttaca
     1501 gacgaggagg caactgatgt agcaagaaaa ttagctaaat tagaaggtgt atttgcagga
     1561 ttttcttcag gggcaaatgt gtgtgcagca attaagttgt taaagagcag tgaaaaagga
     1621 aaaaatatag ttttaactct taatgatagt ggtttaaaat atttaagtac tgatttatat
     1681 gaagaattgt aataatagca caatagttat gcaatgtttt ttaaattaag tatatagatt
     1741 taaattataa aa
//
LOCUS       DAEPLF010000363         1712 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.365, whole genome shotgun sequence.
ACCESSION   DAEPLF010000363 DAEPLF010000000
VERSION     DAEPLF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.365"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..517)
                     /locus_tag="KR314_003629"
     CDS             complement(<1..517)
                     /locus_tag="KR314_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBH0329943.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSP
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKN"
     gene            678..1331
                     /locus_tag="KR314_003630"
     CDS             678..1331
                     /locus_tag="KR314_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBH0329944.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(1489..1665)
                     /locus_tag="KR314_003631"
     CDS             complement(1489..1665)
                     /locus_tag="KR314_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329945.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT          584 a          212 c          213 g          703 t
ORIGIN      
        1 tattttttaa ttggtcagcc attgaagtca gacttaataa ggctatcaat aaatcatctt
       61 tattttctgg atttatacta atattttcaa aattagactc tataaagtcc ggtatattct
      121 ttctttgatt ttcttttatt aacatgaatt tttcgtaaca tgaaactaaa tctttttcac
      181 tgaaattatc cttttcatac acaccatgta agattctttt aatttcttgt attgtaagtg
      241 tatttttcat agaataaata attatcatct gtaaaatttg ttctttagta tacttttttc
      301 ccttaactgg tttgattaat ccctctttgc tgtaattatt tatcatggtt ttagtaagaa
      361 ttttatcact ttcaaatctt ttattaggag aaagtttatt gtcaattaga gaaataattt
      421 gatccatata taagtcaata gagggaatat cgtttgaagt tatatctgca ttatttagtg
      481 tttctagtat tatagtatta agttcattat tcatcatatg ttacctccat aatatatata
      541 atacactaaa ttagaaagta aaacaatatt aagacttgta aaaaaaacat aaagtggtat
      601 aattaaatat ataatatagt aatgaaaact atatttatga atctgatttt taaaatacat
      661 aattgtagga ggacgaaatg tatcaatttt ttttaaaggg tagagaccca ataagcagtt
      721 taactcattt tataggtgct tgtttatctc ttttagcaac tataatttta gtttttcaat
      781 ctgttacatt acaggaaact tctctcttga tgatagtatc tgtatcggta tttggacttt
      841 cactaattgc tttatatagt gcaagttcgt attatcattt tttaaagggg acaccagaac
      901 aagaattgtt ttttagaaaa gtagaccatg caatgattta tgtattgatt gctggctctt
      961 atacaccaat atgtctgaat tttatggaaa aaaaagaagg cataatattt gttacagcaa
     1021 tatggatagt tgcatttatt ggcataataa taaaaatatt ttggatggat gcacctagat
     1081 ggttatctac cagtatatat ttacttatgg ggtgggcaat tgtttttgat ataaatgcat
     1141 tcaattcaat tcctaaggat tgccttagac tattaattat ggaaggtgta tcttactcta
     1201 taggtgcaat tatttatata attaaaaagc ctaatataag tcctgagttt ggatttcatg
     1261 aaatatttca tatttttatt atgataggtt ctttatttca ctttttagca gttttattgt
     1321 atgttttata gttttaaaag atttgattaa ttgatactaa aattctacaa tgaataaaat
     1381 ggaactgcct tatatagatt atatcttcga caataagaca gttccttatt ataaatatta
     1441 aattctactt ataataaaat tttgataaaa atagtccatt caaaatgcct attccataat
     1501 tcctttaatg tacttttcat ttacgacata tagtttgaat ttatgtacta taattatatc
     1561 ctctaaattc tctgaaacac taacaattct gacacatttg tttctaattt ctacaggtaa
     1621 atctaaatca tttgttaaat tcacatagtc atcatattta tacataaaat catcccttta
     1681 cattaagata tacttattat actatatttt aa
//
LOCUS       DAEPLF010000364         1688 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.366, whole genome shotgun sequence.
ACCESSION   DAEPLF010000364 DAEPLF010000000
VERSION     DAEPLF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.366"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            67..1332
                     /locus_tag="KR314_003632"
     CDS             67..1332
                     /locus_tag="KR314_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBH0329946.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
     gene            1371..>1688
                     /locus_tag="KR314_003633"
     CDS             1371..>1688
                     /locus_tag="KR314_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329947.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKV"
BASE COUNT          588 a          209 c          290 g          601 t
ORIGIN      
        1 aaaaagtaat actatatata gtactagcat taggcacaaa ataatacaaa tggaggtact
       61 tatattatgg aatttcaaca aataatagct atatgtatat ttttaatagt tatggcagct
      121 attattacag aaaaggtaaa cagatccgta gcagctgttg gtggtgcact attaatgata
      181 atattcaata tacttacact tgatgaagga ttaagccata tagatttcaa tactattggt
      241 gtattagttg gtatgatgct atttgtagct gttgtcaaaa actcagggtt atttgaatat
      301 atagctatct ggactgcaaa gaaagctaaa ggtgacccat ggaagataat gatttgtttt
      361 gctattataa cagctatttt atcagctgta ttagataatg ttacaaccgt cttacttatt
      421 ggaccaatga ctatagttat tacacaaata ttaggactta atccagttcc atttttaata
      481 acacagattt tagcttcaaa tataggagga acagctactc ttataggaga ccctccaaat
      541 attatgattg gtagtgctgc caatcttagt tttatggatt ttgtaataaa tttaggtcct
      601 gctgttatag taatacttgc tataactata atatgcttta gatttattta tggaaaagaa
      661 cttgttgtga atgaaagagc taaaaatgca atattaaaac tagatgaaaa aaaatctgtt
      721 aaagataaac ctctacttat aaaaagcctt atattaatag cattcatact ttttggtttt
      781 atgtttcaca gtacaatcca catagattct tctgttgtag cactgacagg agcatctata
      841 atgcttttaa taggaaaaca agatgtagat gaaattatgg caggtattga atggtccaca
      901 attttattct ttatgggatt atttgtagta gttggtggac tagtagaagt tgggataata
      961 aacaaattgg ctcaagcatt gattggactt acagaaggac atcttgtatt tacaatgttg
     1021 ttgatattat ggttatcagc tatagtctct tctttcttag ataatattcc atttgttgca
     1081 acactaattc ctcttatttt gactatgcaa gctgaaggta ttgatgtaat gccactatgg
     1141 tgggcaacat ctcttggagc ttgtcttgga ggaaatggaa cactaatagg agcatctgct
     1201 aatgttgtac tagcaggtat aggtaataaa cacggtcatc ctatctcttt taaggagtat
     1261 ttcaaaatag gttttccact tatgattata tctattatta tatctactgt ttatttaatt
     1321 ataaaatttt aaaaaatata gtataataat actaaagggg atgtgtatat atgaatatta
     1381 atactgttca aggtgattcg attgaagttc ttttaagaca gcttggtgcc acaagaatat
     1441 caaaagtaag tagtacatta tattttatca agtttgattt aggagacggt tgggaaatat
     1501 cttatactta taatattaat gctaaagacc aatatttttt acagagaatt gaaccatatc
     1561 ctattggtag aggtctattt aatgatgaat atgaaatagt atcatttata tcaaaggatt
     1621 taaaaaaatt tctaaatgct aaaaatagta gtaactttaa gacatttgtt gaagttacta
     1681 gaaaagtc
//
LOCUS       DAEPLF010000365         1653 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.367, whole genome shotgun sequence.
ACCESSION   DAEPLF010000365 DAEPLF010000000
VERSION     DAEPLF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.367"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            55..762
                     /locus_tag="KR314_003634"
     CDS             55..762
                     /locus_tag="KR314_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0329948.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            809..>1653
                     /locus_tag="KR314_003635"
     CDS             809..>1653
                     /locus_tag="KR314_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0329949.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADSREKQKIMGMILSVVIITG
                     LLIFYVTSISLSLVDILKQINQMEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFFNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFMFIGQISSRIKYKNLILIIGSIILMLLYTLLMTRLNSMSVEMLKN
                     SVSISETIRKIYPPIYYFVDALKTGSVVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGY"
BASE COUNT          635 a          177 c          258 g          583 t
ORIGIN      
        1 atttgttata tattatatat aacaaatatc aaaactataa tatggaggga aattatgtta
       61 aaagtaatca atgtatctaa atcatataaa aataaaaaag tagtaagtag tgtctcattt
      121 gatgtaaatg atggagagct ttttgggttt ataggacata atggagcagg taaaactact
      181 actattaaag caattgttgg aatacacgat tttgaagagg gagaagtact aataaattct
      241 aaatctataa aaaaacagcc tgttgaatgt aaaaaggaaa tggcatatat acctgataat
      301 ccagatttat acgagtcatt aacgggtata caatacctta attttatagc tgacatattt
      361 gaagttgcca aagacaaaag agaagagtta ataaaatatt attcaaataa gtttgaactc
      421 aataaagcat taggagattt aatatcttca tattcgcatg gtatgaaaca aaaattagct
      481 ataatttcgg ctttgataca cagcccaaaa atcttaattc ttgatgaacc ttttgtaggt
      541 cttgacccaa aagcttcctt tatattgaag gaaattatga gggagttttg tgacaaaggt
      601 ggttgtatat ttttctcaac acacgtttta gaagttgctg aaaaaatttg tgataagatt
      661 gccataataa aagatggaaa tataattgct tatggaacta cagaagaagt taagggaagt
      721 aattcacttg aaagtatctt tatggaatta atagagaaat aaaatgtttt ttatacaata
      781 tagtgtctaa agtgaggaat gataaaatat gagcaatcta agtatattaa taaaaactaa
      841 tataataaat gaatttaaat taaatgcgtt aaaaaaagct gatagtagag aaaaacaaaa
      901 aataatgggg atgatacttt cagttgttat tataacaggg ttattgattt tttatgtaac
      961 tagtataagt ctttctttag ttgatatttt aaagcaaata aatcagatgg aaatgctact
     1021 tatatttgga tttggattat caacattaac aactattgtt acatcattat ataaaacgtc
     1081 atcatatctg tttcaagcaa aagatttaga ccttttgact agtttaccta taaaagaatc
     1141 tactgtacta gcaagtaaga tattaatgtt ggttggagca aactatttat tttcttcaat
     1201 atgtatgatt ataccagcag tggtttattt ttttaattct gatgtaagta taacgtattt
     1261 cccttatttg atactgctat ttttagcttt accattatta ccaatagttg cttcatcaat
     1321 aatttttatg tttataggac agatatcatc aagaataaaa tacaaaaatt taatattaat
     1381 tataggtagt attatattga tgttgcttta tactctactt atgacaagac ttaactctat
     1441 gtcggttgag atgttaaaga atagtgtatc tatttcagag acgattagaa aaatatatcc
     1501 acctatatat tattttgttg acgctcttaa aacaggaagt gtagtttcct ttttagcatt
     1561 tatagcaatt tcaataatac cattttttat gtttgtaaca ttatttgcaa aaaaatttaa
     1621 agatataaat gctaagatga gtgaaggata taa
//
LOCUS       DAEPLF010000366         1644 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.368, whole genome shotgun sequence.
ACCESSION   DAEPLF010000366 DAEPLF010000000
VERSION     DAEPLF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.368"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..553
                     /locus_tag="KR314_003636"
     CDS             <1..553
                     /locus_tag="KR314_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329950.1"
                     /translation="KIKLIDRCLIIVMAILMFQSIYNLFVNEVNSQDTTTIDVVVRTT
                     SAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLPPKGQESSQNNIMNVKNTIGFT
                     SEADNYEKKIPINNNEGFEEGETSELQIIIATVICIAALTVLFVVRNFTTTTSASLGT
                     ISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(740..1612)
                     /locus_tag="KR314_003637"
     CDS             complement(740..1612)
                     /locus_tag="KR314_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0329951.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
BASE COUNT          590 a          199 c          220 g          635 t
ORIGIN      
        1 taaaatcaaa ttaattgata gatgccttat aattgtaatg gcaatattaa tgtttcaatc
       61 tatatataat ttatttgtta atgaagtaaa ttcacaagat actacaacta ttgatgtagt
      121 agtaagaacc acttcagcag ctatatttgg gtatttttta agtgctaatt ttataaagag
      181 acctttaagg aagtatacaa atactgatgt atcttcaaat ctttttatag ataataattt
      241 acccccaaaa ggtcaagagt ctagtcaaaa taatataatg aatgtcaaaa ataccattgg
      301 atttacttca gaagctgata attatgaaaa gaaaatacct attaataata atgaaggttt
      361 tgaagaagga gaaactagtg aattgcaaat tattatagct actgtaattt gtatagcagc
      421 attaacagta ctttttgttg taagaaattt tacaactaca acatcagctt ctttaggtac
      481 tatttcacag atgagagatt ttgtctcagg atgcgtgggt tttttgctag gttgtccaag
      541 taaacctgat taaaaaagta ttatcaataa aaggtatttt aaaaagtatc agtataataa
      601 tatttagatt taaatgtata caattataat tctataacat tctatattgt atttttatac
      661 tgatactcta aactatacta tttattaaaa tattttaaca ttgtatttaa acaaaatatt
      721 atatctgtat aatataaatt tagtttatat gaatagatgc catatactca ataaaaagtt
      781 tcatactatg agatatgtat tgatttcttc tccaaagaac tccaatattt gcagtaattg
      841 cacgttctaa tggtattgaa cagagatttt ctgtattttg aaatatttct ttatacaata
      901 ttgttgaagc caagtcattt tctagtaaat aacgtatggt agaaagttga tttgagtgta
      961 aaattatatt tggcttagct ttttcaagtc tttttgtaat aatcttatag tgataagaac
     1021 cttcagataa gattacaaaa ggtacatttt ttaacatata atttgatata atgggatgtt
     1081 ttgccaattc atttttggga tttgtaacta agtacaattc tgttttatag attgtctttg
     1141 aatctatatc atcataaata tcactgtcaa ttataccaat acaaaaatca agtgtagatt
     1201 cttcaatcat tttagctcca acaaatgttg gaacttcaaa aattttaagc tctatatctg
     1261 gataaattac attaaatcta ggaagtattt ttgaaagcaa aaacgtacct aaaactgtag
     1321 gaatacctat tttgagagaa gcttttctct tttttcctaa atcaagtgcg tcacattgaa
     1381 aatcctcaaa ttgttttata aaatgttttg acttgtctaa aaaaattttt ccttcaggcg
     1441 ttaattctat tttattacct attctgtaaa aaagttcaaa gccaagttct ttttctaatt
     1501 ctttaattgc taatgttatg gatggttgag aaatatgaat actttccgca gccttagtca
     1561 tgtttttata tttacagaca gtttgaaaat agtataaata gtttatattc atttggtatt
     1621 agttcctttc atttacatat tagt
//
LOCUS       DAEPLF010000367         1629 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.369, whole genome shotgun sequence.
ACCESSION   DAEPLF010000367 DAEPLF010000000
VERSION     DAEPLF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.369"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(219..935)
                     /locus_tag="KR314_003638"
     CDS             complement(219..935)
                     /locus_tag="KR314_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0329952.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSREIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(1476..>1629)
                     /locus_tag="KR314_003639"
     CDS             complement(1476..>1629)
                     /locus_tag="KR314_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBH0329953.1"
                     /translation="YEKVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAI
                     LHTPNL"
BASE COUNT          600 a          221 c          188 g          620 t
ORIGIN      
        1 aaaaacttga taatataata cctatgcaga caaactctaa ttctactttt gacaaatgta
       61 caggagtacc gaaaaaataa aaataaggga gagcaaactc tttataataa caattaatat
      121 tataagaaaa gttattattc aaatcataaa ctgcttcaaa taatatagaa actaatagaa
      181 acagtaataa taataaaata aattttgctt tagaatcatt aataattaag tttttcaatt
      241 ttgtaaccaa tgccccacac cacctttaaa tattttggtt cttttggatt aatttcaatt
      301 ttttctctaa ttcttctaat atgaacagca atagttctct cacaactata agcatcttca
      361 ttccagacat tttcataaat ttcacgggaa gagaaaattt taccttttcg tgacataaga
      421 agtgtaagta ttttatattc aagtggtgtc attttaattt cctcaccatc taaagaaact
      481 atttttgtat ctgtatttag ctctaggcca ccacttttta gaatataatt agtatcattt
      541 gagttatttg tattactaaa atcacctaaa tttatatacc ttctaagttg agattttact
      601 cgagcagtaa gttctagtgg attaaatggt ttagttatat agtcatcagc acccatatta
      661 agacctataa ttttgtccat atcttctgat tttgcagata agagaattat tggtatattc
      721 cttttttctc ttattttaag tgtagtttta agcccatcta aattgggcat cattacatcc
      781 attattatta acttaatatc attttcgatt agtatgtcca atgcttcaag tccatcatat
      841 gcctttaaaa cattaaagtt atcaggtctc aaatagaact ctatagcatc aactatctct
      901 ctatcatcat ctacaaccaa tatattttga ttcaatttat taaccccctt gaaattttat
      961 ttttaaccaa tttttatttt ataccaaaaa gaggtattag aaaacagtaa gcccgttcat
     1021 tatgaaataa aaactagtac ttttaatata ttgtcaatgc atatagtaaa tttaaaagta
     1081 tagacgccca tattaatctg aaggtaaaag tttatgaaaa tatctagggt aatctatcac
     1141 actccttatg ttattttggg tttattaatt ttttaggttt aaacatatat taacaaatat
     1201 aaataacaaa tgagtttcat aattattaag aatttataac aaatatttat tttaatgtag
     1261 tttaatataa ataggtagtt taatgtagat atgtataaag tatttaataa ggcttatagg
     1321 ttttaatgat gatatcttta tatattttaa gagatgatga aattagatat gggtaatagt
     1381 tgaatgagaa aaagtagtat agcacaaaaa gtgctatatc acaaaatctc aaataatttc
     1441 cttcttataa cttaaattaa aagaataaat atttcttata aattaggtgt atgaagtata
     1501 gcaggaacat ctttattatt taataactta tcaaatgatt tatggtcaga cttatagcca
     1561 ataccttctc tatattctaa ttcatcaaca attttgtatt gttgacaggc atttttaacc
     1621 ttttcatag
//
LOCUS       DAEPLF010000368         1625 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.370, whole genome shotgun sequence.
ACCESSION   DAEPLF010000368 DAEPLF010000000
VERSION     DAEPLF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.370"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            201..941
                     /locus_tag="KR314_003640"
     CDS             201..941
                     /locus_tag="KR314_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBH0329954.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            953..>1625
                     /locus_tag="KR314_003641"
     CDS             953..>1625
                     /locus_tag="KR314_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBH0329955.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNE"
BASE COUNT          734 a          107 c          273 g          511 t
ORIGIN      
        1 tgattataaa ctataaagct tatgtaaagt aaataaatta tgtaaaattt aggttaattt
       61 tgtataaaga gatactttta tattttacaa ttaataacca aatactaaaa aatattttaa
      121 ataaagaact taaaattgtg ataaaattaa tttatagata ttaaaaaaag tataatatat
      181 ggtttttaag aggtgctaag atgaaaaatt ttagagattt aggtgggaac aaaactgagg
      241 atggcagaac tgtaaagaag ggattatttt ataggtctgc aaaattatca aatctaagtg
      301 aaaatgatat aaagatttta aaagagttaa atattaaata tatatttgat tatagaagtg
      361 atgaagaagc tcgtaaacat ccatcaacaa taatatcaaa tataaaaaat ataagaatac
      421 cagcaatgag agagctagaa gaatcaggag gaagttttgg ttctattgaa gatatgattg
      481 atggattgtt tgaaaaagat ggtgcattta atatgttaaa taatagttac tataatttgc
      541 ctataaataa tccgtcatat aaaaaattag tagaacttat aagagactat tctaatttac
      601 caatattaaa tcattgtaca gctggtaagg atagaactgg tgtggggagt gctataattc
      661 ttatgatttt aggggtatcg agagagaata taatgaaaga ttatctaaaa agtaatgatt
      721 ttgcagataa agagatagaa agatttatag aatataagcc taaatttaag gacattccaa
      781 aagaaaattt aaaatatata tttggtgtaa atgaagagta tatgaaaact gcatttagaa
      841 gaattgatga agaatacatt agtgtagaag catatttata tggagaattt aatttaaata
      901 aagaagaaat aagaaaatta agaaatcaat atctagagta aaaggtgagt ttatgagttt
      961 aaatataaca gcaagacaga taaatataat aaaaatattg ttaaattcaa aagatacaat
     1021 tagtggtatt gccttaagtc aagagatagg atgttcttca aaaactatac aaaatgaaat
     1081 aaaggatatt aataaacaac ttagaaatgg taaaattttg tcaataagag gagttggtta
     1141 taaactagaa ggaagttttg atgaggtaaa tctaaatagc aatctctatg atgatgtaga
     1201 tagagtagaa tatataatca gaaaaatatt aaccttaagt aatgaagaaa aaaatactat
     1261 aaaacttgaa gatttagccg attctatgta tgtaagtgtt tcaacagtaa aaaatgattt
     1321 aaaagaagta aaaagaatat tggaaaagta ttcaatatca gttaaatcta agcataaaca
     1381 aggtatatgt gttttggaaa gtgaagataa gatattaaat tgtatagttg acttaagcaa
     1441 taagagagat aatcaattga gtttaaatga ttttttaaat gacgatataa aggaaagaaa
     1501 actattaata aaaaaattat tattagacat attaaatgaa aatggattag tattaactga
     1561 tatagaattt aagagtgtgt taaataatat attagttatg ttaagcagac ataagtataa
     1621 tgaaa
//
LOCUS       DAEPLF010000369         1609 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.371, whole genome shotgun sequence.
ACCESSION   DAEPLF010000369 DAEPLF010000000
VERSION     DAEPLF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.371"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            64..798
                     /locus_tag="KR314_003642"
     CDS             64..798
                     /locus_tag="KR314_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0329956.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(940..1431)
                     /locus_tag="KR314_003643"
     CDS             complement(940..1431)
                     /locus_tag="KR314_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBH0329957.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT          600 a          195 c          235 g          579 t
ORIGIN      
        1 aacttatatt ttgtatttaa tagagtgttg ctaagtgaat atatttaaaa tggaggaaac
       61 tttatgaaac aaaataaata tgatgatgat agatttttta ataaatacag taaaatggaa
      121 cgttctacaa atggattagc aggtgctgga gaatggcatg ttttaaaaaa aatgttacct
      181 gatttcaaag gaaaaagagt tttagattta ggatgtggat ttggatggca ttgtcaatat
      241 gcagttgaga atggagcaat ttctgctgtt ggagttgata tttcagaaaa aatgttaaaa
      301 gaagctagaa acaaaacgca acttgataat ataaattaca tatgtatgcc tattgaagat
      361 attaattttc caaaagatag ttttgatgta gttattagtt ccttggcatt tcattatatt
      421 caatcatttg aagatatatg taagaatata agtaattgtc tttcaaataa aggtaatttt
      481 gtattttctg ttgaacatcc tatatttact gcatatggaa gtcaagagtg gtactgtgat
      541 aaagatggca ataatctaca ctggccagta gatagatatt ttgaagaagg agtacgtaag
      601 tcaaattttc taggagaaga ggttataaaa tatcataaaa cattaacaac ttatttaaat
      661 acgttaatga aaacaggatt tgaaatatta gaaattattg aacctcaacc agaagaaaaa
      721 ctgttagata caattcctga tatgaaagat gagctacgta gacctatgat gttacttgta
      781 tctgcaaaaa aaagataagt aaatataaat aatcattatt gtagaaaaaa attgtagaaa
      841 ataattacac taatggagat gattatacca gtggtattaa ttacaccagt agtaaggagg
      901 caaaagcatt cgttgctttt gccatttttt aagttgcaat tatacttcta cgtagtatat
      961 ttcgtttcca ttttcttcta taagcttttt catatcttca aattttaaaa atacagtagc
     1021 agtattatca tttggatgta tacctatttt atccattttt aataaatcct tatcaaatac
     1081 aacttttaca acagcctctt tatcatttaa tataccaaaa ggagtcacag ctcctttagt
     1141 aagtttcaag tatttttgaa gtctttcttc tgatgcaaaa ctaagaggtt tactatcaat
     1201 ttgttgacga atatctttta aatctgcttt tttgtcttta cctaatacaa ctaaaaaatg
     1261 ttcttttcct ttagcatcac gaagaaatag gttttttaca acaaattcca tatgtaatgt
     1321 ttttaattct tccatttctt caattgtata aactggtgga tgatttacag cctcataagg
     1381 tatgcttaac tcatctaatt ttttatatac ttcctgttgt tgattgttca tgatttcctc
     1441 ccattagaaa tttattttat atataaataa tattatcaca gaaaacttta aaagaaaacg
     1501 ttttatataa tatagacaat agattatatt tttataaaga agcaagaata ctcaataaaa
     1561 cttaaagaaa ttaaacaatt tctaaaaata ccttgaaaat agaacctta
//
LOCUS       DAEPLF010000370         1591 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.372, whole genome shotgun sequence.
ACCESSION   DAEPLF010000370 DAEPLF010000000
VERSION     DAEPLF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.372"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            289..1350
                     /locus_tag="KR314_003644"
     CDS             289..1350
                     /locus_tag="KR314_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBH0329958.1"
                     /translation="MRDFLKNYKSSIILLACTIIGVIVGLVLGPKASILKPFGDLFIN
                     ALLMIVVPLVFLSVSSGVASIKNMERFGKIMKSTAIVFMGTTLVIAFLGITSALLFNP
                     SKGLDVSSVKSLMVAQDAETLESKGTLLDQVVSSITVKNFGDLFSSSNLLQLVIFAVL
                     VGTAIAMMGDRGNTIRKFLSEGTEAILKVVSIVMLYAPIGLGCYTATIIGELGSKIIQ
                     GYVRVFVIYSLTAAMYFLLFYSLYAFLSAGKQGFRQFWRNAIKPIATALATSSSSACI
                     PSNLEASKNIGVPDDISETVIPLGINIHKDGSVLAVIIKVAFLSGILGSGMANPATIG
                     QLFITVFVVGVFMAPVAGG"
     gene            1363..1560
                     /locus_tag="KR314_003645"
     CDS             1363..1560
                     /locus_tag="KR314_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBH0329959.1"
                     /translation="MLVVSIFGFPVEAIPLLTVITTITDIPNTILNTTGNTVSSMLVS
                     RLVEGKDWLIDKTAITTKKIS"
BASE COUNT          588 a          178 c          280 g          545 t
ORIGIN      
        1 aattaagcaa tattatatat atttttttat attgtttgtt ttattaaaat taatctagaa
       61 aaaaagtttt aaaagcagaa ttttattaat atttatatat agatatgaac tattaataaa
      121 atattaaaga tacaaaaaaa ctatagaaat tgacatatat tccagtattt gctaaagtag
      181 ctgttgggga aaattacaaa aattattaat gcgcattaga taatttactt taattttttt
      241 aggatttagg gtaatttata atataaaaca ttagaaagga aaatataaat gagggacttt
      301 ttaaaaaatt ataagtcatc aattatactt ttagcatgta ctataatagg ggtgatagta
      361 ggattagtac ttggtccaaa agcttcaata ctaaaaccat ttggagattt attcataaat
      421 gctctattaa tgatagtagt accattagtt tttcttagtg tttcttcagg tgttgctagt
      481 ataaaaaata tggaacgttt tggaaaaata atgaagagta cagctatagt atttatggga
      541 acaacacttg taattgcatt tttaggtatt acatctgcat tattatttaa tccgtctaaa
      601 ggattagatg tatcaagtgt aaaaagttta atggtggctc aagatgcaga aactttagaa
      661 tcaaaaggaa cgttattaga ccaagtagta tcaagtatta cagttaaaaa ttttggagat
      721 ttattttcca gtagcaattt actacaatta gttatatttg cagttttagt tggaacagca
      781 atagctatga tgggagatag gggaaataca ataagaaagt ttttatcaga aggaacggaa
      841 gcaatattaa aagtagtaag tatagtcatg ctttatgctc ctatagggct aggctgttat
      901 acagctacaa taattgggga acttggaagc aaaataattc aaggatatgt aagggtgttt
      961 gtaatttatt cactaacagc agccatgtat ttcttattat tttacagtct gtatgctttt
     1021 ttatctgcag gaaaacaagg gtttagacaa ttctggagaa atgctataaa accaatagca
     1081 acggcactag ctacttcttc aagttcagca tgtataccat caaatttaga agctagtaaa
     1141 aatataggag ttcctgatga tatatcagaa acagttatac ctcttggaat aaatatacat
     1201 aaagatgggt cagttttagc agtaataata aaagtggcat ttttatcagg tattttaggc
     1261 agtggaatgg caaatccagc aacgataggt caattattta taactgtatt tgttgttgga
     1321 gtatttatgg ctcctgtagc aggtggctga cttattggtg agatgctcgt ggttagcatc
     1381 ttcggttttc cagtggaggc aattccattg ttaacagtta taactactat aactgatatt
     1441 cctaatacta tattaaatac aactggaaat acagtatcat caatgttagt ttcaagatta
     1501 gtagaaggaa aagattggct tatagataaa acagcaatta ctacaaagaa aataagctaa
     1561 ttgtacactt aattaataaa gttaataggt a
//
LOCUS       DAEPLF010000371         1563 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.373, whole genome shotgun sequence.
ACCESSION   DAEPLF010000371 DAEPLF010000000
VERSION     DAEPLF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.373"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            176..1531
                     /locus_tag="KR314_003646"
     CDS             176..1531
                     /locus_tag="KR314_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0329960.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATVLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
BASE COUNT          533 a          177 c          230 g          623 t
ORIGIN      
        1 aaataagata tatttataag acccttaaaa tcactattat ttaataaaat ttataattat
       61 atactatttg ctgttgacat tggtattata ccatagttta tcatttatat tagaaataaa
      121 attattaatt aatagtttcg taattttata atagatttac agggagtgat aataaatgga
      181 tacaaaaata tctataaacg aaaacatgag tctaggtaaa agatttttta aatacctagc
      241 accatctgta gttgcaatgt gggttttctc actatataca atggttgatg gaatatttgt
      301 aagtaaagga gttggagaat tggctttagc tgctgtcaat atatcaatgc cttttattaa
      361 ttttatattt gcagtttctc tattattttc aactggtgca tctaccataa ttgcaatcta
      421 tctggggaaa aaagatatta aatctgctaa cgaagtcttt tcatttaact tagtatcaat
      481 aattatatta tcaataatta tattagctat aacttttttt aatttagata gattagcact
      541 atttttagga gctacagaat ctacaattgg tatggttaaa gattatttag gaataataat
      601 attttttaac ggattcttta tagtgtctta ttctttagaa gttataataa aaactgatgg
      661 ctttccaata ttagctactg ttggtgtaat aatatctgct cttacaaaca tcatacttga
      721 ttacttgttt gtaattgaat ttggatgggg tgtaaaaggt gctggtattg caactgtttt
      781 gtctcaagtc ttttctacta ttttcttctt aatacatttt ttaagaaaaa actctacttt
      841 gaatttttca aaatttagaa ttgattttaa gacacttaga aaaattgtat ttataggttt
      901 tcctgattca actactgaat taagttgtgg tatagttgtt ttacttttta acttaagtct
      961 tactaaatat attggagaaa atgctcttat ttattatagt gtcataaatt atataaatac
     1021 attagtactt atgactatga tgggtataac tcaaggtatg caaccactta ctagctttta
     1081 ttatggtgca ggaaatatag ataatgtaaa aaaattatta aaaatgggga taaaagccac
     1141 aataatagca tcagttgctg tatttgcaat ttgtatggct ttttcaggac caatagtctc
     1201 tttatttata cacccagaag aaacaatgct attcaatgaa ggagttcgtg tatttaaaat
     1261 attctctata tctttcttat tggtaggaat taatgtaata atttctggat tctttgtatc
     1321 agtagaaaaa ccatcaattt ctactgttat atctcttggt agagggcttg taatagttgt
     1381 tttaagtcta atttcaatga tactaatatt tggtggacaa ggaatatgga tgactactat
     1441 tgtttctgag tttatatgtc ttattttatc attagttttt ctaaaaaaga atttttctac
     1501 attagactct aatctaaata aggttgctta gatgataaga gtagcaatat atgaaaatat
     1561 cta
//
LOCUS       DAEPLF010000372         1549 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.374, whole genome shotgun sequence.
ACCESSION   DAEPLF010000372 DAEPLF010000000
VERSION     DAEPLF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.374"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(39..1382)
                     /locus_tag="KR314_003647"
     CDS             complement(39..1382)
                     /locus_tag="KR314_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBH0329961.1"
                     /translation="MVPNTNNVQPDGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(1455..>1549)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT          564 a          269 c          231 g          485 t
ORIGIN      
        1 tataaattta aaatattgac ctagaactat ttaaaatatt aacttagaac taagtttaca
       61 actgcaagta aacctataat ccaaagtgta atactgattt tatcagtttc tttagctacc
      121 acatgcatta ttatatatga aataaatcca aagcataaac caatacttat actataagtt
      181 aaaggcatca ataaaactgt aacaaaagca ggtacagcta ttttgctatc agcaaaatca
      241 atctgtttta tgctttcaaa catgaataca ccaaccatta caagtgcagg agctgcagcg
      301 taagtaggaa ctataccaag caatggtgta aatataagtg tagctaagaa taatgctgct
      361 gttactattg aagcaagtcc tgttcttgca cctgctgcga taccagaagc agattctcca
      421 aatgttgtaa cagtactagt tcctaagaat gagcctatta aagtagaaga aacatctgca
      481 tataacattc ttcctaaatc ttttatttca ccattctcat cttcaagacc catttgttta
      541 caacaagtaa taagaaatgc taatgaatca aacatgtcta taaacatgaa agagaatata
      601 ggtccaatta atgagaattt caatgcacct acaatatcta atttaaaagc aactggtgct
      661 atactaggag gtgttgagat tattgctgtt ggcaactcca caaatccaaa tataacagat
      721 attattgttg caactactat acttatcaat attccacctt taactttttt atattctagt
      781 attgccatta agataagagc aaaaagagct attaaaactg ttggtgtaaa atctgcaagt
      841 gaaactatag tatctggatt agctattata acacccatat tttttaaacc aacaaaagca
      901 aggaaaagac ctatacctgc tgaagatgct actgccaatt ctctaggaat tgcgtctgca
      961 attgtctttc gaagacctgt tactgcgagt gtaaagaaga cacatccaga tagaaaaact
     1021 accccaagac ctgtttgcca tgggattcct gcacctataa ccaaagagta tgtgaaaaat
     1081 gcatttaatc ccataccagg agctagagca aatggtgcat ttcctaaaat cgcaaatata
     1141 agtgtaccaa tggctgtagc tagtatggta actgttataa gggcggcttt atccattcca
     1201 gcttcagata atattgctgg atttacgaaa actatgtatg ccattgttaa gaatgtagta
     1261 actccaccta ttacttctgt tttaaggcta gagccacgtt cgctaaattt aaacattttg
     1321 tctaaaaagc ttaatgatga attatttcca tctggttgta cattgttagt gtttggtacc
     1381 atttttacct ccaaaaaact ttaagatata aacaaaaaaa tatatattca tatagataga
     1441 atagaacata tattcaaagt tgatattcta ttcatagaaa acccatttaa ggtgagtttg
     1501 tagaaactat tagaccgtat tactaatatt atatgaacat tttctattt
//
LOCUS       DAEPLF010000373         1537 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.375, whole genome shotgun sequence.
ACCESSION   DAEPLF010000373 DAEPLF010000000
VERSION     DAEPLF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.375"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            274..1464
                     /locus_tag="KR314_003648"
     CDS             274..1464
                     /locus_tag="KR314_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0329962.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
BASE COUNT          592 a          169 c          223 g          553 t
ORIGIN      
        1 tgactatatc aactatatta tattaaacaa ttaatatagt caagaatatt ttattctcta
       61 aatctatgtt ttattaattc tttaaaaaac tattatataa aataatcatg ataaaattag
      121 aaattggaat ttttaatatg cactgtacat aaatatattt tatataaaag tttatatttt
      181 caaacaagta atttttcgaa aattgtctaa attcagtaaa aatatggtaa tatatacata
      241 agcaatataa atctataaaa aggagtgtta catatgatat ctaataaaat gcaaacttta
      301 gttgcaaata gttctgttat aagagctatg tttgaagaag gaaagaaact ttctgatatt
      361 tatggtgagg aaaatgtttt tgattttagt ataggaaatc caagtgttga acctccagag
      421 actattaaag cagttataaa tgatatttta aatgaagaat ctccaaattt agttcatgga
      481 tatatgaaca actcaggata tgaggatgta agagatgcta ttgctgaaca tataaataaa
      541 aaagatggat taaatcttac aagagaaaat ttaattatga cttgtggtgc tgctggtggt
      601 ctaaatatta ttttaaaaac tcttttaaat cctggtgatg aagttattgc ttttgcaccg
      661 tattttggtg agtataaaaa ttatacagaa aactatgatg gaaaattaat agaagttcct
      721 acaaatatag agacttttga accagattta gatgctctaa aaaatgctat tactccaaaa
      781 acaagagctt taataataaa cactccaaat aatcctactg gtgtcatata ttctgaagaa
      841 ttattaaaaa atcttggaga attattggat tcaaaacaaa aagaatttaa tacaagcata
      901 tatctaattt ctgatgagcc atatagagaa ataatttatg atggtgccaa agttccttgt
      961 gtactaaaat actatagaaa ttcatttata gggtactctt atagtaaatc attatctcta
     1021 cctggtgaac gtataggata tattgttgct aatgggcaaa tggatgattt tgatgatgtt
     1081 atgtcttcat taaatgttgc aaatagaata cttggatttg taaatgctcc ttctcttttt
     1141 caaagggtta ttgcaagaag tttagatgca gaagttgatg taaatattta taaaaagaat
     1201 tttgaccttc tatataatag tttaatcgat atgggatatt cttgtgttaa acctaatggt
     1261 acattctatt tgttcccaaa agctccaatt gaagatgata aaaaattctg caatgatgca
     1321 aaacaattta atttattatt agtaccaggt tcttcttttg gttgtccagg tcattttaga
     1381 gtatcatatt gtgtatctta tgataaagtt aaaagttctt taccagcatt tgaaaaatta
     1441 gccaaattat ataatttaaa ataactaaat caaagtaatt caaataatta actaccttag
     1501 tatagaatac ttacattcta tattaaggta gtttata
//
LOCUS       DAEPLF010000374         1534 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.376, whole genome shotgun sequence.
ACCESSION   DAEPLF010000374 DAEPLF010000000
VERSION     DAEPLF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.376"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..473
                     /locus_tag="KR314_003649"
     CDS             <1..473
                     /locus_tag="KR314_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBH0329963.1"
                     /translation="HNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYY
                     IPYFEDENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNL
                     SSSFKLIKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            579..1238
                     /locus_tag="KR314_003650"
     CDS             579..1238
                     /locus_tag="KR314_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBH0329964.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            1241..>1534
                     /locus_tag="KR314_003651"
     CDS             1241..>1534
                     /locus_tag="KR314_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBH0329965.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKL"
BASE COUNT          634 a          173 c          204 g          523 t
ORIGIN      
        1 aacataactc actttcagat gctgtggatt taatgaacgt atgtaaatgc tttttcttta
       61 actcccctaa aaatatcgat attcttgaag aattatataa taaattacct gctctcaaaa
      121 cacctttata ttatattccc tactttgaag atgaaaattt tgaactcaaa tttgatagaa
      181 ctccagaaga tataataata tatcttaaac aaatattaac tatactaaat ataagtaaaa
      241 aagaaattta ttttaaaaaa agaagtgtat tagtgaataa ctctaaaatc attaacttta
      301 aaaatctatc ttcttctttc aaattaataa aactcaatga atttgaagaa tatcctgatt
      361 ttatcttaaa attaggagat aaaaagagtt ttgttgaatc tgttattaaa ataaataaaa
      421 gtaacagaaa atccataaaa aatttaatat taaaattatc aaaaaaacct taaatagttg
      481 aatccaatta tttaaggttt tttatacttt gaattaaatt ttttatatag gatatactaa
      541 ctaagtgtaa atatttttta aaggagtaat tgatagatat gacagtagta ttaataagaa
      601 gtattatatt atacataaca gtgctaattg cacttagagt catgggaaaa ggtgaaatag
      661 ctgaaatgaa ctgttttgat ttagttataa cactattaat tgcagaagtt gcatctgtcc
      721 caatggaaaa taataatata ccaatcataa atggtgttgc agcaatatct ggtcttgtaa
      781 tcatgcagac tcttatttca ttcttatcat taaaaagcag aaaattaagt tcatttttat
      841 caggtaaacc ttctgtatta atagataaag gaaaaattgt atataaagag cttaaaaaag
      901 aaagaattag tatagatgaa ttattagaac aacttaggat tcaaggatac ttcaacctaa
      961 aagatgtaca atatgctatt ttggagactg atggtaatct aagtgttgta ccagcatcaa
     1021 gctacaatag tactcctcca agagctttta atcatttacc tatcccactt atactggatg
     1081 gtagaataat aaataaaaat ctagacatag ctcaaaaaga tactaactgg ttaatgggta
     1141 ttttaaaatc taatcacata gaaactttta aagatgttct tatttgtgtt ttagatgaaa
     1201 atgacaaaat atttattcaa aataaaaagg gtgattaata ttgaaatcta caacatttgt
     1261 tatactatgg actatcttat ttgtattatt tggattttac gttaacaata aactatatga
     1321 ttttacagaa ggatataaag acaatatatc tgttttagaa aaatctatag aaaatgaaga
     1381 ttgggaaaaa gctcaaaaag aagctgattc aatatctacg agttggaata aagaaaaaaa
     1441 ccattggtac aaagttttaa atcatgagta ctttgatgaa ataggattaa ggtttaatat
     1501 tctggacaaa gcaatttata ctgaaaataa atta
//
LOCUS       DAEPLF010000375         1532 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.377, whole genome shotgun sequence.
ACCESSION   DAEPLF010000375 DAEPLF010000000
VERSION     DAEPLF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.377"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(93..1040)
                     /locus_tag="KR314_003652"
     CDS             complement(93..1040)
                     /locus_tag="KR314_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBH0329966.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKAKYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDFVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEAIKKIK"
     gene            complement(1009..1455)
                     /gene="lspA"
                     /locus_tag="KR314_003653"
     CDS             complement(1009..1455)
                     /gene="lspA"
                     /locus_tag="KR314_003653"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBH0329967.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
BASE COUNT          525 a          271 c          146 g          590 t
ORIGIN      
        1 aatactttaa tataagatat tttatttaaa tttcactaaa tttatttact tatataaaaa
       61 caaaaaatga tttataagta ttatacaaat ggttatttta tcttcttaat agcttcctta
      121 aaatactcag gtaattctga attaaattcg atatattcct tagtggtagg atgtataaat
      181 cccaacttct tagcgtgcaa agtttgtcct tttaatttaa atttagtttc ttttggacca
      241 tatacaaaat cacctaaaag tgggtgattt atagacaaag catgaactct tatttgatga
      301 gttctaccag tttcaagcct agctctcata tgtgtaaact tatcaaatct ttctacaact
      361 tcaaaatgag taattgcatt ttttccatcc tttactacag ccatttttaa tctatcctta
      421 ggatttctcc ctataggttt atcaacagtt attttatctt ctttaaccac accatagcat
      481 ataaattcat actctctagt tatagtatgt tcctttaact gttctgctag aaaattatgt
      541 gcataattgt tcttagctat cataagaaga ccagaagtat ctttgtctat tctatgaact
      601 ataccaggtc taataactcc atttattgac gacaacttat ctttacaatg atataaaatc
      661 ccatttacta gtgtattttc atagtttcca ggagcgggat gaacaaccat atcctggggt
      721 ttatttatta ttaagacatc atcatcttca tatacaatat ctattggaat atcttgaggt
      781 aaaacctcta atactttagg ttctggtatc tctataacaa tattatcttc ctctttaact
      841 agatatttag ctttttctac tttattattg acctttactt ttccatcttt tataattttt
      901 tgtatataac ttcttgacat atcacctaat tgttctgcta gataaatatc aagtctaact
      961 tcttcttcct cttcaagtac taaaaactct tttacttcat ccattatatc acctactttt
     1021 actttcaaaa aataaaacat atatacataa aaacacagtt cctacaacta caaatacatc
     1081 agctacattg aatacatatt cccatataac tctaaagtcg aagtaatcta ccacaaaacc
     1141 taatcgtact ctatcaatta gatttcctaa tgcaccagat ataattaata taattcccaa
     1201 ccttccaaat atatgtactt tccttgtatt aagatagtat aatccaaata ctgttgcaag
     1261 taatgcaaca attataaata tccactgatt attttgtaat aatccaaatg ctgcacctct
     1321 attttcgaca taagttaaat gaaatacatt atttattatt ggtattgttg aaacatctac
     1381 caaattattc aatacccata ttttagacag ttggtctaag cctatgagta gaattattat
     1441 taatatatat agcaatgatt tttcccccta taaattattt aaaaaccaat tttatttgaa
     1501 acacactatt attattatac aaaaaaaatg ct
//
LOCUS       DAEPLF010000376         1527 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.378, whole genome shotgun sequence.
ACCESSION   DAEPLF010000376 DAEPLF010000000
VERSION     DAEPLF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.378"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(52..1344)
                     /gene="brnQ"
                     /locus_tag="KR314_003654"
     CDS             complement(52..1344)
                     /gene="brnQ"
                     /locus_tag="KR314_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBH0329968.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFALSYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVDVPVV
                     KSLPLHSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT          549 a          277 c          190 g          511 t
ORIGIN      
        1 ttttagttaa ctcatttatt tacaattgat tatattttaa ttaaacaaaa cttagtttgt
       61 tccaagtgta ttagattgac tctttgatgg tataaaatta ccaattattc cagctattat
      121 aactggtacc acccagttaa atcctaatga atgaagaggt aaagatttta ctactggaac
      181 atctactcca taactagata taactgttag tatacttaca agcaatgaca tatatactgc
      241 tcctttgtac acattatcat tcttaatttt ttcattgaag aaagctaaaa ttattaaaac
      301 aattgatggt ggataaacta tacttagtat aggagatgct atctttatta tagttccaac
      361 accaaaagat gctactactg cactgaatac agatacagct aatactattt tttcataact
      421 caatttacct tttgtaagtc tagagaaata ttgacctgtg gcagatgtaa gacctataga
      481 tgtagttaaa catgctaatg atactactac tgctaaaatc gctttcccaa catttccaag
      541 taatccttca gttatattta ctattacttg tgattgtact gcatctgttc catacattgt
      601 tgatacagtt gctccaagga aacataatcc tccataaact agagctaatc ctccacaagc
      661 aagaaccccc gccttaccaa tcatttttaa cttgtcacta atagtagaat atcctttatc
      721 atttaaagat actattaaca ctgatgcaag tgctattgct gcaaaagcat ccattgtctg
      781 atatccttga cctatacctt ctgcaaatac attaggtatc ataggtttat ctactatatc
      841 tccaagaggt gatataatac cttttataat tataaatgct agagctatta aaagagctgg
      901 agttaaaaac tgtccaacta tatctacgac cttagaaggt cttattgtta gtacatatga
      961 aagcgcaaaa aaaagtatac agaataataa cactggaatt gaagttccaa ataaaggcat
     1021 tatacccatt tcataagttg ttgcacctgt tcttggtata gctaagaatg gacctataca
     1081 taaaatgtct gcacaaccta taagaatccc taacttcata ccacatcttt taaacatttc
     1141 aactacgttt ccatcgaatt tagctgtagc cattactgct agaagtgcca atccagcatc
     1201 agcaaacgta aatcctaaga aggcaattaa ccattctgga cctgtaatta ttcccaagta
     1261 aggtggaaat attaagttac ctgctccaaa gaacattgca aataacgcaa atccaaatac
     1321 tataacatcc ttggtcttac ccattataaa atcccccttt ttaaccttat taaattaata
     1381 aaatgtctcg attgtttacg cttgcataaa taaattaaat taaacaataa gaacttatta
     1441 ttaaattaat aaaattaaat aaattaaatt tttaatatat tgtatttatt tgtgcttatt
     1501 atatattata cgaaacaagc aagtcaa
//
LOCUS       DAEPLF010000377         1484 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.379, whole genome shotgun sequence.
ACCESSION   DAEPLF010000377 DAEPLF010000000
VERSION     DAEPLF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.379"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            271..1365
                     /locus_tag="KR314_003655"
                     /pseudo
     CDS             271..1365
                     /locus_tag="KR314_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          513 a          175 c          272 g          524 t
ORIGIN      
        1 tattcaaata tatattgact attcagaaaa atatgactaa tatataatct atagagaaac
       61 taattttata tataatgact acttagataa aagaatctaa aaaaattatg agaggaggag
      121 tagggtataa aatcagatta taagttttaa gttattatac taaaaagcta taaaacagtt
      181 atcatgtatt ttatgttaaa ggaaattgta ggtagtttat cgaattattt aatcagaaga
      241 aaaaatttca catggggagg taactgagat atgtcagaaa aagtaaatgt taaaacagtc
      301 atcagtttcg caggtgcgta tgtagcaaca gtaatcggtt ctggatttgc aacaggacag
      361 gaaataatgc aattcttctc tttttatggt tttgcaggta ttattggtgg gataatttca
      421 atggtattat tctcatggat gggtgcttca gttatgtcaa aaggtaaaga gcttcaactt
      481 aaagaaccta tcaaaattta tcgtgtttat tgtggtaagt atttagggat attctttgaa
      541 tggtttggac ctttattctt atttggtgtt ttcgtagtaa tgatttcagg agcaggagca
      601 actttaactg aatactatgg attaaatcca tttgtaggta gagtaggtat ggcagtagta
      661 gcattattaa ctgtttcact aggtcttgat aaattatcaa agatactagg tggaataggg
      721 cctataataa taatctttac acttttagtt ggtggtataa gtttagctaa aaacataggt
      781 aatataggag aagctgcaca agttcttagc actgtaaaga tagctaaacc agttccaaat
      841 gcctatctat ctggtgtaat atatactact tataacacaa ttgttgttat ggcattctta
      901 actggattag gagcaagtgc tgctaacaaa aaagaagcta tttgtggtgg tatattaggt
      961 ggagtagcac ttatggctgc agctataatg atgcacttag ctattctttc tgatatagga
     1021 aacttatatt caaaagcaat accatcatta ttcttagcag ataagatttc accagtaatt
     1081 ggagttttat tctcaataat tcttatatta ggtatatata caacagctgt acctttatta
     1141 tggtcagtaa ctaatagatt tgtagatgat aaacatccta agtttaaatt aataacttta
     1201 atagcagcta tattaggtct tataggtggc ttcctaccat ttgataagct ggttggtatt
     1261 ttatagccat atactggata tatgggcgtc ataatactgg tttgtgttct ttatagacag
     1321 attacaaaaa catctggata tcaagaagga atcagtgaca aataagtatt ttttaatatt
     1381 gtaaactcaa gagctatatg aaaacaatga aaatgttttc atatagctct ttttattttt
     1441 tagctcgttt attatgaaat aggaaaaaca tgttttttag ttac
//
LOCUS       DAEPLF010000378         1453 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.380, whole genome shotgun sequence.
ACCESSION   DAEPLF010000378 DAEPLF010000000
VERSION     DAEPLF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.380"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            21..503
                     /locus_tag="KR314_003656"
     CDS             21..503
                     /locus_tag="KR314_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBH0329969.1"
                     /translation="MDTKKYKDKLLKEREKLTNLVVDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDSALSKIENGTYGFCDLCHNQI
                     DLERLDILPDTNLCNNCAKKENALLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGTLRD
                     "
     gene            553..1332
                     /locus_tag="KR314_003657"
     CDS             553..1332
                     /locus_tag="KR314_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0329970.1"
                     /translation="MKKAIWGICICLLSIVGVYTYNHNTHNQNSSKETYTLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQILDILKKNNVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYGSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDTIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
BASE COUNT          637 a          146 c          235 g          435 t
ORIGIN      
        1 taaaattaga ggtgttgaaa atggatacta aaaaatacaa agataaatta ttaaaagaaa
       61 gagaaaagtt aactaattta gtcgtagata tgaaagataa taccttgttt ggagacacta
      121 ctaaacatac tagtgaaaaa tattcatcag gagaattatc tagttatgac aatcatattg
      181 gagatatggg aacggattta tatatgcaaa atatgcaaaa tagtttaata aatcatgaag
      241 aaggaagact gtatcaaata gattcagcat tatctaaaat agaaaatgga acatatgggt
      301 tttgtgattt atgtcacaat caaatagatt tagaaagatt agatatatta cctgatacaa
      361 atttatgtaa caattgtgca aaaaaagaaa atgctttatt aggtgatgca atgcaaaatc
      421 ctgatgtaga agattctaac ttttattcag aagacctaac aaatttaact gatttaaata
      481 aaaatggaac attgagagat tagaagcctg acatcatata ttgttagaat gaattagaaa
      541 agaggtaatg acatgaagaa agcaatatgg ggtatatgta tatgtttatt atccatagta
      601 ggtgtatata catataacca taatacacat aatcaaaata gttcaaaaga aacctatact
      661 ttaaatgaca atataaaaga cgattatgaa gatgtaataa taaaaaaagg aaatgaggat
      721 gaaaagattc tagcattgac ttttgatgat ggtccagatg aggattttac accacaaata
      781 cttgatatat taaagaaaaa taatgtaaaa gcaacttttt ttgtagttgg agaaaaagta
      841 gaatataaca aagaattatt aaaaaggcaa tatgatgagg ggcatgaaat agggaatcat
      901 acatttacac atataaatgt tgcaaaaaat agttatggta gtgttgaaaa agaaataaca
      961 gatacacaaa atattataaa aagtgtaata ggagtagaac caaagatatt tagacctcca
     1021 tatagagcca tgagtaaaag tgtctgtgat attatagttt ctaaaggcat gaatataatt
     1081 ctttggtcaa acttagaccc tagagattgg tctaaccctg gtgtagatac aattataaat
     1141 actattctta caaaggtcca aaatggtaat ataatacttt tacatgatta taacactaaa
     1201 agaaatgata aatcacaaac tatacaagct ttagaagtag taattcctaa attaaaagag
     1261 agaggatata aattcgtaac agtatctgaa cttatacaac atttagataa aaataagcaa
     1321 gtgcaaaagt aaaaatattt gtttgaagat tagtggtgct tgtaagttta aagtacagac
     1381 atcattaatc tttaatctat aaaacacaca aaaagtatta attagtatgt tatttttaaa
     1441 ttttttatac ata
//
LOCUS       DAEPLF010000379         1432 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.381, whole genome shotgun sequence.
ACCESSION   DAEPLF010000379 DAEPLF010000000
VERSION     DAEPLF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.381"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            163..>1432
                     /locus_tag="KR314_003658"
     CDS             163..>1432
                     /locus_tag="KR314_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329971.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMR"
BASE COUNT          604 a          122 c          264 g          442 t
ORIGIN      
        1 gaaattaaca ttaattttag ttttaaattt taaatttatg gtttataatt agtataggga
       61 tattctgaaa tataataatt ttccagttta tcccaatatt taacttaggt agattgctta
      121 caaaacaaat gattatatat taggctaaaa ggaagtggat agatgatatt tgtaaaaaat
      181 aagaaataca gaaaaattgc cataattata tctattccta tattgcttgt tgcgcagtac
      241 taccttttta aatttggaat acttagacca atggtaaagg gagtagaaat tgaaattgta
      301 gatggggaat atgttaaaga tatagataaa tatgtagtaa aattaaatga aacagttact
      361 ttgtctacag gtgaatatat taaggttcct tcgtatgcta aaaatccaaa tatttggttt
      421 aatgtattgg ataactcagg gactttaaaa atagatggaa ataaaatgac agcattaaag
      481 gttgggtatt cttctgtggg tatcatgaaa aattcaagag tattaaaaaa ggctactata
      541 aaagtagtag acccagaggt agaatcacta gatatggaga ttaatgggga tttaaaacat
      601 gttggtgata gtgctactat agaaagtaca atagaagttg attatgataa gtttaaaaaa
      661 tcatataagc cagtttataa gagtagcaat gaaaatattt taaaaatcaa aggaaataaa
      721 gtaagtgcag ttggagttgg taaagctaca atatatgcta agtgtggaaa taaagaaatt
      781 gagagaacat ttaggattca agctagagtt gctgaaataa atatggatga tataaaaata
      841 gaagttgacc aaaatgaaaa attgaaacca gatataataa cttctccacc tggtttagaa
      901 ccaccaaaaa tatattatga gttagtagat tcaaaattac ctgtagaaag agcaataagt
      961 ttaagtagta atggaaatgt tgtaggtctt agagaaggta aagatagggt gagaataact
     1021 tgtggagata aatctaaagt aataacagta acagtagtca aagaaactat aacgaatgac
     1081 tatatacaaa acttggttgg ttcacataaa atagtaaaca ataaagttat tattaaactt
     1141 gaatgggatt atatgaaaga tgtctttgat tatgatatat atcttaaaga taagttgtca
     1201 ggagaatcat ttaagaaaat taaatctata actataaatg aaagtgattt aggtaagtct
     1261 aacaaggtat atgcaactat agaagtagat atgaagggta atcaaaatat agattttgat
     1321 atttatgtca tagggaaaaa ggattcagat acaactaagc caagtaatgt aataaatatt
     1381 actaatggaa atggaaattc aaataatgat aataatatta aagatatgag gg
//
LOCUS       DAEPLF010000380         1422 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.382, whole genome shotgun sequence.
ACCESSION   DAEPLF010000380 DAEPLF010000000
VERSION     DAEPLF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.382"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(158..373)
                     /locus_tag="KR314_003659"
     CDS             complement(158..373)
                     /locus_tag="KR314_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329972.1"
                     /translation="MHINQTGFISNGLGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVREGSSWAFNFIVQP"
     gene            complement(556..900)
                     /locus_tag="KR314_003660"
     CDS             complement(556..900)
                     /locus_tag="KR314_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329973.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            1027..1410
                     /locus_tag="KR314_003661"
     CDS             1027..1410
                     /locus_tag="KR314_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329974.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
BASE COUNT          512 a          191 c          187 g          532 t
ORIGIN      
        1 tgttaaaata aaattcatat attaagaaat attaatagta aataagcaat tgttactata
       61 acgtaggaat tatctttaaa aacacaaatt aatttacatt ctctaatttt aaagtatatg
      121 tatatctttg attttaataa aattcactta aaatatttta aggttgaaca atgaaattga
      181 atgcccaaga acttccttct cttactaatg ttgcaattaa ttcaaaccca caattgtatt
      241 gaactgaatc tctacctact ggtttccaac tagaaggtgc attatatgag aaagaatttg
      301 atgttggctc tttcttttgt tgctgcttaa ttcctagccc attagaaata aatccagttt
      361 gatttatatg cacccttcta tttgttacac gaatctggcc gtctgcaata gaccagtttc
      421 cagaaacttt actaacggaa atatattgaa atgctccatc tccttttgat attgtatatg
      481 taactgtacc agatgctttt acaccaccag ttttatccca tccatcttct gatatacttg
      541 cttttctaag aggctttatt gtattagtat caaaaaaact gtcttagaat atatctttga
      601 tgttaaattt ttagattgtt taacttctaa taattgagtt gttgatatag aatcaacgta
      661 tatggactct cctgttcttg catttttgat tatacctttt tcattggaac ttttaacatc
      721 agtaattcca tttttagcca tatcgtataa tttgtccatg tctgttactt gtttctctga
      781 atacattact ttcatagatg aatcttctac cttactagca cttgcatttg ttggtaatag
      841 tattgtcatt atgagtaaag ataaaatagt tgttacagat ttttttaacc tataattcat
      901 attaatcctt actttttatt tgttataaca gacattctat tgtaatagaa tataaatcca
      961 caatattttt aaaattagta ttgaataact attctcatgt tataattatt caaaggaggg
     1021 attgatatga ggaaaaaatt ttttataggt attcttatag ttttaataat ctctgtgata
     1081 gctatactta atataaaaaa tgataaaaat gtctttattg gagaatcaga aaattggaaa
     1141 gctaaatttg taactactga aggacactca aaattagaaa taaaatataa aaatagtaaa
     1201 gatgtagttg aacaaaaatt taattattcc tatcaattta tagatggtga agatgcagtt
     1261 gtagttgtaa aaaatgaaat tttagaagca ggtcaagaaa aagcaataaa aatacaaaga
     1321 ccaggtcttt attcaaatag agaatctagt tacaagctaa ttatatcttg gaatggaaat
     1381 gaggaaacta taaacttgaa aaagaaataa ccatattatc aa
//
LOCUS       DAEPLF010000381         1407 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.383, whole genome shotgun sequence.
ACCESSION   DAEPLF010000381 DAEPLF010000000
VERSION     DAEPLF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.383"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            32..1312
                     /locus_tag="KR314_003662"
     CDS             32..1312
                     /locus_tag="KR314_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBH0329975.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKVDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
BASE COUNT          596 a          180 c          165 g          466 t
ORIGIN      
        1 ataaaataat taaagataaa aggagtattt tatgaacata cttggattaa tagttgaata
       61 taacccattt cataatggac acatatatca tttatctaaa tctatagaaa aaacaaatgc
      121 aacacatact gtagcaataa tgagtggaaa ttttctacaa agaggcgaac cagctttatt
      181 tgataaatat actagagcaa aaattgctgt cgaaaatggt gtagacttag ttatagaatt
      241 accaacttta tttgcttgtc aaagtgctga aattttttct catggtgcta taacaatttt
      301 aaattcacta aactgcataa attctatatg ttttggtagt gaagaaggaa atacagaaat
      361 tctttataat atagctgaaa ttttagcaaa tgaaccaaat gaatttaagt tacttttaaa
      421 aaaatattta gatgatggcc tgttatttcc aacagctaga gctaatgcac tttttgatta
      481 tatacatcaa gacaaaaaaa ataattttat acaaaattat tcaaaggaac aattatcaaa
      541 aacattaaat tcatcaaaca acatactggg tatagaatac ataaaaaacc tcatccattt
      601 aaacagtagt ataaaaccat ttactatctc cagggtccaa tcagaatata attccgaaga
      661 aattgagagt gatatttgtt ctgctactgc tataaggact tcacttaaag aaaatacaga
      721 cctttttcat ttaaaaaatg ttattccaga aaaaacttat aacatcctaa atgaaaaagt
      781 agataatggt tttttaccta tgtttgatga cttcttcttt gatattttaa aaacaattat
      841 acttagagat atggacactt taaaagagta ttttgaagta aatgaaggca tagaaaacag
      901 aatctataaa gatatcttta catctgaatt tctacatgag ctacaattag caattaaatc
      961 caagcgatac actttaacca aaatcaaacg aactctaaac aatatattgt taggtattaa
     1021 aaaaagcgac atgcaactta taaaaaacat agatactata ccttatataa gaatacttgc
     1081 ttttaatgat aaaggtagag aaattctaaa agagataaaa aataaatctg aaataaaaat
     1141 aataaacaag ttttcaaata tatcattttc attagatgat acgattttta aaacactcat
     1201 aagttatgat ataaaagcta caaatatata taattcaata tattataaaa acaataaaga
     1261 tttagtaaaa ggccctatgg atttttatac tcatccaata tatgtaaagt agttttttaa
     1321 aataactata tttataaaaa tcattttatt aactctttat tatataagat acaatagtct
     1381 tactttattt atgtacacat gctttta
//
LOCUS       DAEPLF010000382         1397 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.384, whole genome shotgun sequence.
ACCESSION   DAEPLF010000382 DAEPLF010000000
VERSION     DAEPLF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.384"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            54..1379
                     /gene="cdeA"
                     /locus_tag="KR314_003663"
     CDS             54..1379
                     /gene="cdeA"
                     /locus_tag="KR314_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBH0329976.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GISFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKGRYKY"
BASE COUNT          449 a          173 c          212 g          563 t
ORIGIN      
        1 tagttactat atagcttatt atagatagag agtaataaaa ggaggtaaaa tttatggaaa
       61 atttatttac aagaaaattc actacttttg aatttctaaa atttgtttct cctgcaatta
      121 tatccatgat atttatatct ttgtacacaa taatagatgg catctttgta tcaacattag
      181 ttggttccga tgctcttgct agtataaata ttgtactacc tataattaac cttgtttgtg
      241 gatttggaat aatgatggca acgggtggag gtgctatcgt ttctatacgt atgggtgaaa
      301 atagacagga tgaagccaac tctacatttt cttttatagt tttgttttca ttgattgttg
      361 ggatttcatt cacagtaatc tcatatttct ttatcaaaga aatatctatt ttgcttggtg
      421 caacagataa gttattacca tattgtataa cttatggtaa agttatgatt ttatgtacac
      481 cattttatat tttaaaattt atatttgagt actttgcaag aactgatgga aattctaaat
      541 ttagtttatt tctatcagtc attggtggtg taacaaatat aatattggat tatgtattta
      601 ttaaatattt tggaatgggt cttttaggag ctgcagttgc aactgctata ggtattattt
      661 taacttgtgt tttaggtatt atttacttct tatctaataa atctacactc aaattaagaa
      721 aaccaaaaac cgattttaga cttataagag atactatgat aaacggttct tctgaaatgg
      781 ttacagaatt atctacagga attacaacat tcttatttaa tgtagtagct ttaaaattag
      841 caggagaaaa tggacttgct gctcttacta tagtattgta tgctcatttt ttaatgacat
      901 cagtctatct aggattcgct gctggagtgt ctccattaat aagctataat tttggtgctg
      961 aaaacagtga taaattaaaa gaaacattta aacattctct aaaatttata tttgtttctt
     1021 ctcttttagt gtttattatt gctttagtat ttgcaccatt tattgttagg gtttttgtaa
     1081 gtccagataa cacagtattt aaactagcct tacaaggatt aaaaatattt gcatttgctt
     1141 ttttgtttgt tggtataaat atatttgcat caggattttt tacagcattt cacaatggaa
     1201 aaatttcagc tattatatct tttagtcgtg cctttgtttt tataatcata ggaatcataa
     1261 ttcttcctcc tatgttaaac atgactggat tatggcttac agttccattt gctgaggtta
     1321 taaccatatt tatatctatt ctatttataa aaaaatataa aggtagatat aagtattaaa
     1381 aattaatgct taaaatc
//
LOCUS       DAEPLF010000383         1370 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.385, whole genome shotgun sequence.
ACCESSION   DAEPLF010000383 DAEPLF010000000
VERSION     DAEPLF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.385"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(51..719)
                     /locus_tag="KR314_003664"
     CDS             complement(51..719)
                     /locus_tag="KR314_003664"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBH0329977.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(786..1214)
                     /locus_tag="KR314_003665"
     CDS             complement(786..1214)
                     /locus_tag="KR314_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBH0329978.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
BASE COUNT          435 a          267 c          135 g          533 t
ORIGIN      
        1 tagacctttg caaaaattag gattatataa taggtatttt atttatgtaa ttattttatt
       61 tctaaatatc catagccatc ttttattaaa gttcctttta cttcatattc tacaggtttt
      121 ttaaatattg gaccatctac gacaaatgta tgatactctc catgttctcc acatgggtct
      181 gcaccagcat tttttatctt ctccacaagt tctctagtta atttttttcc taaaaattcc
      241 tctcctaagt gctttaagtt tacttttgtt acaacactac aaaatccact gtctataaat
      301 tcatatacta aatcctctct actctcttgc cataatggta aatctacttt cataccaact
      361 gcttcacatc tatcagtatc ccattttcta tgactttcta tatctatatc tccatatgca
      421 catatcgttg ctcccatgtt tttagctttt attaaagccc tctcaaagct ctcttcatat
      481 ccttctacac cacactcagc aactaataaa ggtaatccta tattgctact tacttgctta
      541 agcatattat attctaaatt atgtgtccat gactttccat gttcttcact tatagttgtc
      601 aagagtgcca caggcttata tccttttttc aacattcgat ttaatgctaa tatactatcc
      661 ttaccaccac taaatgacat tacaaatttt tcatcagcca attttaacac ctctttcatc
      721 taactttgtt ttattaacat aattatacca aatttttgag ttgcatagca aattatatta
      781 aattgttaat tcactataaa gaatgtccct aatattaaaa gtataacccc tatgtatttg
      841 tataaatgca ttgtttcttg tcccaaaatt acgctatcaa taaaaaatcc aactaaaact
      901 tgggatacaa gtgttaatat aagtgtattt gatactccta aatttggaat tgcctttact
      961 gtaaaatata ttattaaacc tccaaatact cctcctaata acaacttata attaaaacca
     1021 tttatagata ctagacactt taagtcacct gtaactatta tacttgccaa aaaaaatatt
     1081 gctcctacag ttaaacttat aaaggtggcc actaatgctg ttgtactttt tcctagttct
     1141 ccatttataa aagcttcaag agctgtagaa ccacctgcta ataatgcaaa tattattgcc
     1201 cataattttg tcatggacaa acctccttct atataattct attaatagaa gtctatgata
     1261 caacctatta attaattact aagtatagtt taatttttat acactaggct ttatttggat
     1321 atgaatttct gttataattg ttctatcgat tttggaggtg attttatgta
//
LOCUS       DAEPLF010000384         1358 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.386, whole genome shotgun sequence.
ACCESSION   DAEPLF010000384 DAEPLF010000000
VERSION     DAEPLF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.386"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..>1358
                     /locus_tag="KR314_003666"
     rRNA            <1..>1358
                     /locus_tag="KR314_003666"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          387 a          282 c          403 g          286 t
ORIGIN      
        1 gcgacctacc catgagcagg atgaagcgaa agtaaaattt cgtggaggtc cgaacccacg
       61 agcgttgaaa agctcgggga tgacttgtgg gtagcggtga aattccaatc gagcccggag
      121 atagctggtt ctccccgaaa tagctttagg gctagcctca aggtgagaga tacggaggta
      181 gagcactgaa tgtcctaggg ggtattgcac ctaccgaaga ctatcaaact ccgaatgccg
      241 tcatcttata cttgggagtc agactgtggg tgataagatt catagtcgaa agggcaacag
      301 cccagatcgt cagctaaggt ccctaaatgt aagttaagtg gtaaaggatg tgggattgca
      361 cagacaacca ggatgttggc ttagaagcag ccactcattc aaagagtgcg taatagctca
      421 ctggtcgagt gatcctgcgc cgaagatttc cggggctaaa acttactacc gaagctacgg
      481 catcagtaat gatgggtagg ggagcttccc atacgggttg aagcatgacc gtaaggacat
      541 gtggacagta tgggagtgag aatgttggca tgagtagcga gatgtgggtg agaatcccac
      601 aggccgtaaa cccaaggttt ccaggggaag gttcgtccgc cctgggttag tcgggaccta
      661 agctgaggcc gaaaggcgta ggtgatggac aacaggttga tattcctgta ctaccgataa
      721 ccgtttgaga gaagggatga cacagtagga taagctaagc acactgttgg ttatgtgtgc
      781 ccaagcattg aggcagtcaa agtaggcaaa tccgctttga taatgctggg atgtgatggg
      841 gagcgaaatt tagtagcgaa gtagctgatt tcacactgtc aagaaaagtc tctatcgagg
      901 ttaaaggtac ccgtaccgca aaccgacaca ggtgggtgag gagagtatcc taaggccagc
      961 gagagaactg ttgttaagga actcggcaaa atgaccccgt aacttaggga taaggggtgc
     1021 caccatcagg tggccgcaga gaataggccc aagcgactgt ttaccaaaaa cataggtttc
     1081 tgctaagtcg caagacgatg tataggagct gacgcctgcc cggtgctgga aggttaaggg
     1141 gatctgttag agcaatcgaa gcagtgaact taagccccag taaacggcgg ccgtaactat
     1201 aacggtccta aggtagcgaa attccttgtc gggtaagttc cgacccgcac gaaaggcgta
     1261 acgatttggg cactgtctca acaacagact cggtgaaatt gtaattccgg tgaagatgcc
     1321 ggatacctgc gacaggacgg aaagacccca tggagctt
//
LOCUS       DAEPLF010000385         1336 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.387, whole genome shotgun sequence.
ACCESSION   DAEPLF010000385 DAEPLF010000000
VERSION     DAEPLF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.387"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            27..101
                     /locus_tag="KR314_003667"
     tRNA            27..101
                     /locus_tag="KR314_003667"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KR314_003668"
     tRNA            116..200
                     /locus_tag="KR314_003668"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            209..292
                     /locus_tag="KR314_003669"
     tRNA            209..292
                     /locus_tag="KR314_003669"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:243..245,aa:Leu,seq:tag)
     gene            322..398
                     /locus_tag="KR314_003670"
     tRNA            322..398
                     /locus_tag="KR314_003670"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Arg,seq:tct)
     gene            406..481
                     /locus_tag="KR314_003671"
     tRNA            406..481
                     /locus_tag="KR314_003671"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:439..441,aa:Gln,seq:ttg)
     gene            570..658
                     /locus_tag="KR314_003672"
     tRNA            570..658
                     /locus_tag="KR314_003672"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:604..606,aa:Ser,seq:tga)
     gene            662..737
                     /locus_tag="KR314_003673"
     tRNA            662..737
                     /locus_tag="KR314_003673"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:695..697,aa:Phe,seq:gaa)
     gene            744..817
                     /locus_tag="KR314_003674"
     tRNA            744..817
                     /locus_tag="KR314_003674"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:778..780,aa:Met,seq:cat)
     gene            832..908
                     /locus_tag="KR314_003675"
     tRNA            832..908
                     /locus_tag="KR314_003675"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:866..868,aa:Ile,seq:cat)
     gene            932..1008
                     /locus_tag="KR314_003676"
     tRNA            932..1008
                     /locus_tag="KR314_003676"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:966..968,aa:Pro,seq:tgg)
     gene            1016..1092
                     /locus_tag="KR314_003677"
     tRNA            1016..1092
                     /locus_tag="KR314_003677"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1050..1052,aa:His,seq:gtg)
     gene            1101..1176
                     /locus_tag="KR314_003678"
     tRNA            1101..1176
                     /locus_tag="KR314_003678"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1134..1136,aa:Lys,seq:ttt)
     gene            1184..1257
                     /locus_tag="KR314_003679"
     tRNA            1184..1257
                     /locus_tag="KR314_003679"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1216..1218,aa:Cys,seq:gca)
     gene            1264..1335
                     /locus_tag="KR314_003680"
     tRNA            1264..1335
                     /locus_tag="KR314_003680"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1296..1298,aa:Asn,seq:gtt)
BASE COUNT          320 a          310 c          355 g          351 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaatgcgc ggatgtggcg gaattggcag acgcactaga
      241 cttaggatct agcgccattg gcgtgggggt tcgactccct tcatccgcac catttttgtt
      301 tgatatacat atataaattg tgggtgcata gctcagctgg atagagcaac gcccttctaa
      361 ggcgtgtgtc cggggttcga atccctgtgc gctcaccaca tatattgggg attagccaag
      421 tcggtaaggc acatgacttt gactcatgta tgcgtaggtt cgagtcctgc atccccagcc
      481 aaatgatgta ttactaagct atatttgaac tatttttatt ttaaatatag ttaggttcaa
      541 ttttagaata agtttaagta tccgaatgtg gagaggtgtc cgagtggttt aaggagctgg
      601 tcttgaaaac cagtgacttc gaaaggggcc gtgggttcga atcccaccct ctccgccaaa
      661 tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg gtggttcgat
      721 tccgcctctg ggcaccaaaa tatggcggta tagcttagtt ggctagagcg ttcggttcat
      781 acccgaaagg tcataggttc gactcctatt accgctacta aaatttaatg tgggccatta
      841 gctcagttgg ttagagcgcc cggctcataa ccggtaggtc tggggttcga gtccctgatg
      901 gcccaccaca ttaaattaat taatataaat tcgaggtgta gcgcagtttg gtagcgcaca
      961 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaat ataaggtggg
     1021 tatagctcag ttggttagag cgccagattg tggctctgga ggtcgtgagt tcgactctca
     1081 ttatccaccc caattcatat ggtctattag ctcagtcggt agagcacctg acttttaatc
     1141 agggtgtccc gcgttcgagt cgcggataga tcaccaaaaa ggtggcgaca tagccaagtg
     1201 gtaaggcagt ggactgcaac tccttgatcc ccagttcgaa tctgggtgtc gcctccaaaa
     1261 tattccaagg tagctcaacg gtggagcaac cggctgttaa ccggtaggct gtgggttcga
     1321 gccccaccct tggagc
//
LOCUS       DAEPLF010000386         1324 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.388, whole genome shotgun sequence.
ACCESSION   DAEPLF010000386 DAEPLF010000000
VERSION     DAEPLF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.388"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..303
                     /locus_tag="KR314_003681"
     CDS             <1..303
                     /locus_tag="KR314_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0329979.1"
                     /translation="KTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKL
                     MTTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFEDIAVEQIVDGIRFTPMKLLI"
     gene            complement(672..1229)
                     /locus_tag="KR314_003682"
     CDS             complement(672..1229)
                     /locus_tag="KR314_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0329980.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
BASE COUNT          457 a          189 c          141 g          537 t
ORIGIN      
        1 aaaacactct tcttttgggg ttgttttgac tctaaaaaac ttattggagt cttagctatt
       61 agaaatttaa atcatatatg tatgttattt gtagataaaa actatcataa acatggtgtt
      121 gcaaaaaaat taatgactac aggcatacaa tactgtattt gtaataaatt taatttaact
      181 gaaataactg taaattcatc accttatgct gttgaagttt atcataaatt aggatttgaa
      241 gatattgctg ttgagcaaat tgttgacggt atacgtttta cacctatgaa attgttaata
      301 taataaatta aataaaaagc aaagtctctt aaatatttta tagttacttt gcttttaaac
      361 caagatttaa aataatacta tcttttttgt tttttaataa tatactttaa aagccttact
      421 aatatgaact ataatttatt ttttgatatc ctttttgatt aacatataaa ttaattcaat
      481 tattaaacat aaactatgtt gtttctcttt atatctcttc tttgtaaaca atattgggca
      541 tttgaaaata aatattctta aaatatactg ttctcctgtt gattttcctt tggtacaaat
      601 gataaaattt taatttacat gctccgtaat gaagtgttta ataaataagt taaaataatt
      661 tatttacctc cttaatcata ttttttatga atacctaata acacagtatc aatagaatcg
      721 gctaaacctt ttatagcttc ttctttactc aaaagtgatt tcccaagttg caaagtcgaa
      781 taaccatgaa gtacactcat tattaagttt aatacttcac ctgtattctt tgtatttaga
      841 ttacaagaaa gaataagttt ttctaataag gatttatatt catcaaagat ttctaatgtt
      901 tcattatttc catgccaagt agcccactga attgtttcat aaacaccagg attagaaatt
      961 ataaaattaa agtatgctat acttatacac tttatagctg tatctccaaa atttccaata
     1021 gtcgccttta tcatttgact attcatctct ctcattcctt tatgagctac ttcaagtaat
     1081 aaatcatcta gactttctat atgattgtac aaagatggtg tacgtattcc tagcttttct
     1141 gcaataactt ttaaagaaac tttgttaagc ccatctttat taactatatc tgatgtagct
     1201 tgtataacta tttctctact aacacgcatg attttccctc tttcatttaa aattaattac
     1261 tttattagtt tagctaatct aattagtttc gtcaattatt gtaatttata aaattcttta
     1321 tact
//
LOCUS       DAEPLF010000387         1310 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.389, whole genome shotgun sequence.
ACCESSION   DAEPLF010000387 DAEPLF010000000
VERSION     DAEPLF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.389"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            203..1093
                     /locus_tag="KR314_003683"
     CDS             203..1093
                     /locus_tag="KR314_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0329981.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSARFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTIQNKDSCEKLLREECARYGILTD
                     MKEIIKAYKRSESYIQIKFF"
BASE COUNT          504 a          124 c          253 g          429 t
ORIGIN      
        1 acagctatta gagattataa ataaatagtt ttaaaggtac tacgaatata gaggaaggta
       61 aatcatacgt tattcaagga ttataaatga gtagtttaaa agtactatcg gataggtctc
      121 tgcatattta aaccttagta aaggtagtta aagtagtagt ttaaaaacta aaaaattata
      181 taaaggaatt aggtataaga aaatgattga gagtatacca gcaaaacaaa ttttacaaaa
      241 agtgaaattt gacaatacaa gatggtttgg aattgactat aatatgaatc tctatcgtgg
      301 ttgttctcat ggatgtatat attgtgatag ccgtagcaca atatacaaca tagagaattt
      361 tgacaaagtt agatataaag aaaatgtgat agagatttta agtaaagaat tgcgttcaaa
      421 aagaaaaaaa ggggtagtag gcattggagc tatgtctgac acatataatc cttttgaaaa
      481 gcaactttgt attacaaagc aagcactaga tttgatttcg gaaaatcatt ttggagtgtc
      541 tattgatact aaaagtagtt tggtggttag agatataccg attttgcaga aaattaaaaa
      601 aaataattct gctattgtaa agttgactat aaccactgca aatgatgaat tatcaaagaa
      661 aatagaacct tatgtaaatc caagttctgc acgatttgac gcagtgaaag aacttaatga
      721 ttctggaatt ttttgtggca tattattgac tcctatgctt ccatttttga ctgataataa
      781 agatgaaata cgagcaattg tagaaaaagc acacaaggct aatgcaaagt ttatttattg
      841 catgtatggt gtaacaatgc gtagtggtca gagagagttt ttttatgagc atttaagaga
      901 tatatcacca aaattagtac ttaaatatca gaaaacatat ggtttaaact atgtatgtac
      961 tatacaaaat aaagattctt gtgagaagtt gttaagagaa gaatgtgcac gttatggtat
     1021 tttgacagat atgaaagaga ttataaaagc gtacaagagg tcggagtcat atattcaaat
     1081 aaaatttttt taagataggc tttggatgag atataatagt ggtgattgat gtgttgatat
     1141 tatagttgat tgttagagag gatattatag atgaatatta atattgatat tctaggtaat
     1201 tgctagaact aatattatag atgaatatta gtattgatat tctaggtaat tgctagagtt
     1261 agtattatag atgaatatta gtattgatat tctaggtaac tgctagagct
//
LOCUS       DAEPLF010000388         1309 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.390, whole genome shotgun sequence.
ACCESSION   DAEPLF010000388 DAEPLF010000000
VERSION     DAEPLF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.390"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(71..286)
                     /locus_tag="KR314_003684"
     CDS             complement(71..286)
                     /locus_tag="KR314_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329982.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(297..764)
                     /locus_tag="KR314_003685"
     CDS             complement(297..764)
                     /locus_tag="KR314_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBH0329983.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFILENVKRFKVMGCCLLINTIIECVIG
                     YNSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          516 a          156 c          141 g          496 t
ORIGIN      
        1 tgtcaataat atagtaaaag aataaaaatc atatagtata ataaaaaaga gtgtttctaa
       61 acactctttt ttaatcattt tcatcttcta catattcaag tatatcacct ggctgacagt
      121 ctaaagcttt gcaaatttcg tttagagtgg taaatctaat agcttttgct tttccagttt
      181 ttaaaattga taaatttgca tttgtaatat ctattttttc tgccaaatct ttaagtgata
      241 ttttcctttt tgccatcatt acatctaggt taactactat tgccataaca tcacctctat
      301 atcgttaaat cattatcttc ttttattttt atagctttat caaatgtttc tgcaattaca
      361 aagcacatta gtgcaaatat aaaagaaata accataagtg gtgagatacc gccactatta
      421 tctccaaata tcattataag actagtattt gagttataac caattacaca ttcaattata
      481 gtgtttatca ataaacaaca tcccattact ttaaaccttt ttacattttc aagtatgaat
      541 ggggaatact tagtactatt aattataaat atcaagaata agatgactat aaagtagtca
      601 gaaaaatata gtattcttgc aattaaagcg attactttgt cagaactaaa atcatataaa
      661 aacataggta tcatacataa gaatattata ccagcaactc ctaaaagtga taatagaaaa
      721 tatagaaata ctgataatat attactaaaa aattttgtaa tcataaaata tcatctcctt
      781 tatataaagt atattattga ttataatata taatttattg aatttcaata ataaattgtt
      841 gatataatat ataaaaccat tataatatac actaaaaata agaatgtata aggtggtaaa
      901 tttatataaa aaagaatatg agttgtaaaa tattaaataa tattgtacta attggaataa
      961 ttttaacttt attatagatt attttaatgc cattagtact gatggtgtat tttaaaattg
     1021 gtataagcat tacaaatacg aatatgccaa ctgtaatatg taatataagt agctatatac
     1081 atatgtgctg tcccatatat tgtatcattg tttatattaa aaagattatg taaattggta
     1141 tcaatcaata aatcattttc agaagaaata cctaaaaact taaaaaatac ctatatgtgt
     1201 atttagttag attggagatt tttacattta attttatagt gacaatatta tattattgtt
     1261 atgatgtcta tttatatgca attactataa tatcagaact tatagtatc
//
LOCUS       DAEPLF010000389         1294 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.391, whole genome shotgun sequence.
ACCESSION   DAEPLF010000389 DAEPLF010000000
VERSION     DAEPLF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.391"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..153)
                     /locus_tag="KR314_003686"
     CDS             complement(<1..153)
                     /locus_tag="KR314_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329984.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARY"
     gene            complement(327..1193)
                     /locus_tag="KR314_003687"
     CDS             complement(327..1193)
                     /locus_tag="KR314_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0329985.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNVEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTREYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
BASE COUNT          408 a          239 c          157 g          490 t
ORIGIN      
        1 gtatctagct atcttaaagg ctaagatgat agaaggatta tacctaccat tttctaatga
       61 acctatagtt tgtcttgata cttgcaaaat agaagcaagt tcttcttgtt ttatgcctct
      121 ttcttttctt atttcttcta atctattttt cacatctatc cccctttaat atctcaacta
      181 cggaaagtct actttccata attatacaac tatatttcat ttatgtaaag cttgctttcc
      241 atatttacaa aaaaataaca gactattttc agcctgtaat tttaaaattt catgttgttt
      301 attaaattta atgttattca ttaactttaa cttggtttct gaattggaat ccaaacttct
      361 gtagtataat caggagcatt gatatcgcca ttgggataaa gttcaatatc tggtaagtta
      421 gcatacacat acccagaact tggtaaccac tcagtaataa ttcttttttg caattcctgt
      481 attgccagag catttggcat tggagaaaca cattcaaata ctgcccatgt acctgatggt
      541 attaagtatt cacgagtatc ttctaataca ggcttatctg ttggtgcagc aatataataa
      601 tctaaaccct tactatttgt acacacacta actccaagca atccacaagg ttcgttattt
      661 attaactctg aaagtttatc aattgttcca ttttttatgc atttagccca taacttagga
      721 actttctcaa agcactcttc tacaatcatg ttcatacttt cttttatacc aacaatacga
      781 aaccctttct tttctacaat acgatattgc atttctacat ttccttttac ataaatgctt
      841 aatgtcatct taggatatgc ttttaaaaca acaccctttg ctctagctgc agatggagaa
      901 atgttatgta tattttgaaa agctctatta aaagatgttg gtgaatcgta accatacttc
      961 tcagatagtt ctaaaatttt gatgttactg ttctgaagtt caaatgcagc cttagtcata
     1021 cgtcttcgtc taatatattc agataatggt acttctgcaa tatatgaaaa cattctttga
     1081 aaatgaaagg ttgaacaaca tgcaattttt gctgcttctg cataatccac tccactttcg
     1141 agattttctt caatataagt aatagcttga tttaatttat ttatccattc catttgatat
     1201 tacctccaag caaattatac agtttaataa aataatatac ctctctattc atgcacaaaa
     1261 ttgtaagcat ttaaattcat aagtattttt caat
//
LOCUS       DAEPLF010000390         1266 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.392, whole genome shotgun sequence.
ACCESSION   DAEPLF010000390 DAEPLF010000000
VERSION     DAEPLF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.392"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..169
                     /locus_tag="KR314_003688"
     CDS             <1..169
                     /locus_tag="KR314_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0329986.1"
                     /translation="LVYFFFGAELQSIDGIVIFKDLKHVKPKLDFINRSIQAPRLEYI
                     TNNVYYFKGEL"
     gene            197..1135
                     /gene="blaCDD"
                     /locus_tag="KR314_003689"
     CDS             197..1135
                     /gene="blaCDD"
                     /locus_tag="KR314_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBH0329987.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT          532 a          107 c          218 g          409 t
ORIGIN      
        1 tttagtctat tttttctttg gagctgaatt acaatctatt gatggtattg tgatttttaa
       61 agatttaaaa catgttaaac caaagttaga ttttataaat agaagtattc aagctcctag
      121 attggagtat attacaaata atgtatatta ttttaaaggt gaactataaa cttatgtaga
      181 ggagatggta taaagtatga aaagaaaaaa taattttatt tggatagtta ttcttctagt
      241 tgttgtagct atgggagtat attatgtagg aagaaagcat aacaacacaa aacaaaaaca
      301 tgataagaat catataaact ctgaattaaa taaatccaaa gttgaaaaca atgataaaaa
      361 caaaaaagaa aaaattaata tggttgacta tagtgactgt tttgaaggta ttagtggagg
      421 agctattttt tataatacta aaaataaaga atataatata tataacaaag aattaattga
      481 aacaagaagg tcaccatgtt ctacttttaa aatagtttca actttaattg gtttagaaaa
      541 aggggtaata aactcaaaag aatcagttat gggatatgat ggtacagagt atccaaataa
      601 aaattggaat aaaaatctta gtttagaaga ggcatttaag gagtcttgtg tttggtacta
      661 caaaaaattg atagataaag ttgatgctaa atctgtccaa aacattttag atgatttaaa
      721 gtatgggaat tgcgatatta gcgaatggga aggagattta aaaaatggaa agggacattt
      781 aaatggattt tggttagaat cttcattaca aatatctcca aaagaacaag tgcaaacaat
      841 ggcaaaaata tttgagggag atacaaactt taaaaaagag catattaata ttttaagaga
      901 tattatgaaa atagatgtaa atgataaaaa cataaatgta tatggaaaaa ctggaacagg
      961 atttgatgaa aaaaataaac gtgtggatgc atggtttgtt ggtatgctag agcgtgaagg
     1021 tgacacctat tattttgcta ttaaatcaga tgattccaat aaagaaatta cagggccaaa
     1081 ggtaaaggaa attgcaatta atataattaa gaaatattat agtgtaaggg aataaactta
     1141 attttatttg taaatattat ttacctatag tctgtaatat ttattaaata attttactaa
     1201 attgagtcta tatagagcaa aactccttta gtacataaaa tacttgctat tgattattaa
     1261 attgaa
//
LOCUS       DAEPLF010000391         1226 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.394, whole genome shotgun sequence.
ACCESSION   DAEPLF010000391 DAEPLF010000000
VERSION     DAEPLF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.394"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..>1226
                     /locus_tag="KR314_003690"
     CDS             <1..>1226
                     /locus_tag="KR314_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase C80"
                     /protein_id="HBH0329988.1"
                     /translation="VNGNLLDNIQLDHTSQVNTLNAAFFIQSLIDYSSNKDVLNDLST
                     SVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEGIPIVSTILDGINL
                     GAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIGAEVTIFLLPIAGI
                     SAGIPSLVNNELILHDKATSVVNYFNHLSESKEYGPLKTEDDKILVPIDDLVISEIDF
                     NNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPYISSHIPSLSVYSAIGIKTENLDF
                     SKKIMMLPNAPSRVFWWETGAVPGLRSLENNGTKLLDSIRDLYPGKFYWRFYAFFDYA
                     ITTLKPVYEDTNTKIKLDKDTRNFIMPTITTDEIRNKLSYSFDGAGGTYSLLLSSYPI
                     SMNINLSKDDLWIFNI"
BASE COUNT          456 a          169 c          189 g          412 t
ORIGIN      
        1 atgttaatgg taatttattg gataatatac agttagatca tacttctcaa gttaatacat
       61 taaacgcagc attctttatt caatcattaa tagattatag tagcaataaa gatgtactga
      121 atgatttaag tacctcagtt aaggttcaac tttatgctca actatttagt acaggtttaa
      181 atactatata tgactctatc caattagtaa atttaatatc aaatgcagta aatgatacta
      241 taaatgtact acctacaata acagagggga tacctattgt atctactata ttagacggaa
      301 taaacttagg tgcagcaatt aaggaattac tagacgaaca tgacccatta ctaaaaaaag
      361 aactagaagc taaggtgggt gttttagcaa taaatatgtc attatctata gctgcaacgg
      421 tagcttcaat tgttggaata ggtgctgaag ttactatttt cttattacct atagctggta
      481 tatctgcggg aataccttca ttagttaata atgaattaat attgcatgat aaggcaactt
      541 cagtggtaaa ctattttaat catttgtctg aatctaaaga atatggccct cttaaaacag
      601 aagatgataa aattttagtt cctattgatg atttagtaat atcagaaata gattttaata
      661 ataattcgat aaaactagga acatgtaata tattagcaat ggagggggga tcaggacaca
      721 cagtgactgg taatatagat cactttttct catctccata tataagctct catattcctt
      781 cattatcagt ttattctgca ataggtataa aaacagaaaa tctagatttt tcaaaaaaaa
      841 taatgatgtt accaaatgct ccttcaagag tgttttggtg ggaaactgga gcagttccag
      901 gtttaagatc attggaaaat aatgggacta aattgcttga ttcaataaga gatttatacc
      961 caggcaaatt ttactggaga ttctatgcct ttttcgatta tgcaataact acattaaaac
     1021 cagtgtatga agacactaat actaaaatta aactagataa agatactaga aactttataa
     1081 tgccaactat aactactgac gaaattagaa acaaattatc ttattcattt gatggagcag
     1141 gaggaactta ctctttatta ttatcttcat atccaatatc aatgaatata aatttatcta
     1201 aagatgattt atggatattt aatatt
//
LOCUS       DAEPLF010000392         1209 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.395, whole genome shotgun sequence.
ACCESSION   DAEPLF010000392 DAEPLF010000000
VERSION     DAEPLF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.395"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..886)
                     /locus_tag="KR314_003691"
     CDS             complement(<1..886)
                     /locus_tag="KR314_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0329989.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEKVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNIFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLWYANLSLEIKKMLSE"
BASE COUNT          397 a          195 c          110 g          507 t
ORIGIN      
        1 cttcactgag catttttttt atttctaaag aaagatttgc ataccacagc aacttatttt
       61 ttatatactc catttctctc tcaattgcat ttttattaaa atttctattc tccaattttt
      121 tctttaattc cttgtattga tttttgtata cattaattat tcccttacaa tccatgctta
      181 tattaaatat attactaaac aatttatttt gaagcaatat ttctgcttct tcaagtctat
      241 tttgctttaa atatatcata gctaaattaa cacttgtatc ccctattgac ttatgtatca
      301 tatttaaata caactctgcc ttttcataat cttccaacat acaatatgcg tttgataata
      361 taataagagc tgtttctttt atttggatat catctgtatt tctcactata tcttcaagaa
      421 tattcgttgt ttcttttact ctgtcattaa gttcactctt accaaattta tatgaataca
      481 tctgatacag cgaacatatc cttagcttca ataaatagct tttaggatac ttctctatat
      541 acatattaga aagctttttt acagattcta agtcttgtct aaattcttct tttggttctt
      601 ctgttgataa atcaatttca attctagcaa agcctgattc taattcttta taaattttca
      661 ttactttttc ttcacttaaa tcaatcttgt aatttaaaag cttatcaata gacacattaa
      721 aaaagtctgc taataaaggc aataattcta tatctgggta agaatttcca ctctcccact
      781 tagatactgc tggtgttgac acacctataa actttgctaa ttgttcttga gttaaatttc
      841 gttcttttct aagacgctgt atgactttac ctattttaat ttgcatatat acctccctag
      901 tacatctgtt gtaatttaag tatataaaaa aaatatttac tagtaaatcg agggaaagtt
      961 aacaaaatgt ctgtaattta accattggtt atattttaat ctaaaaccct atctcattgg
     1021 cttgaaatat aaaatctatt aattaagttt aagtataacc taattaatag attcacatta
     1081 aatagcattt aaatatttag attttctctt tactacaact tactatattt aaactttcaa
     1141 ggaattttaa ataaaataaa ttccatatac aatgataata atactgagtg ttaataattt
     1201 atttataat
//
LOCUS       DAEPLF010000393         1174 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.396, whole genome shotgun sequence.
ACCESSION   DAEPLF010000393 DAEPLF010000000
VERSION     DAEPLF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.396"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            82..>1174
                     /locus_tag="KR314_003692"
     CDS             82..>1174
                     /locus_tag="KR314_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH0329990.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGK"
BASE COUNT          440 a          154 c          188 g          392 t
ORIGIN      
        1 atatattaat cactcctttc ttttaattat tgacaaatat atgctatact aaaagatgaa
       61 tttaaaattt ggaggctcac aatgaaaaga aatttatcac ttttattaat ctgtttattg
      121 atatttactt catttttagg aagctctaat atctcttttg cagataatga accagctata
      181 gttgctaaac atgcagtttt aatggactat gagtctggca aaatactata taataaggat
      241 ggaaattcta agctttatcc agcttcaaca actaaagtat ggacagcttg tttagttcta
      301 aaggaggtta aagatttaaa tcaagtaata gaaattaaag atttacctca aatagatggc
      361 tctagtatgt atctaaaaaa tggggaatct tttactgtaa agcagttact agatgctctt
      421 ttggttcatt ctgctaatga tgctgctttt gtattggcta gatatgttgg tggtggcaat
      481 gtacaaaaat ttattgacct catgaactct gaggcaaaaa aaattggagc tactaatact
      541 cattttaata atcctcatgg attaccagac ccaaatcact acacaacagc tcatgatatg
      601 gctctcatag ctagagaagc tatgaataat gatactttta gacaaattgt aaaaactaag
      661 tcacttaaat ttgaagctac aaaagcatat ccttatgaga gatactttgt aaacactaat
      721 aagtttttga catcacatga taagattaca tacaaaggtc aacctataaa tataaaatat
      781 gatatagttg atggtataaa aactggatat actgatgctg ctggtaaatg tcttttatcg
      841 agtgctgtaa aagatggcag aagagttatc gttgctgtat ttaattcaac taatgctgat
      901 ttatatttag actcacgtat tctgattgat tatggatttg ataactttaa gtgtgctact
      961 attgttgata aagaaaaata cacagatact aaaaaagtat tgtttacaaa acaacatgaa
     1021 ttgatttatg aaccaaaaaa tagttatagg atattcttag aaaaagatga atctcaaggc
     1081 aattatgata ccaaaacaga gttaaataaa attgatttgc ctataaaaaa aggagctaag
     1141 gttggtactt taaatgtata taataatggt aaac
//
LOCUS       DAEPLF010000394         1167 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.397, whole genome shotgun sequence.
ACCESSION   DAEPLF010000394 DAEPLF010000000
VERSION     DAEPLF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.397"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(212..1015)
                     /gene="proC"
                     /locus_tag="KR314_003693"
     CDS             complement(212..1015)
                     /gene="proC"
                     /locus_tag="KR314_003693"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBH0329991.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGINTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT          376 a          216 c          116 g          459 t
ORIGIN      
        1 ttattttttc acaatgatat atctcaaata tagttaaaaa tatatctatt taattattaa
       61 tatttaagta ataaattctc actcaatcat taaattcctt tttatagtaa attactctta
      121 ttttacttat accaaataca tttatgtata gatataaata agtactaaaa aaacaattca
      181 agaaaccata tagtaaactt aattacatct gttatttgct catatcttta gacttatcta
      241 tacaagcaac aatagatttt ataactgctg ctctaaagcc ttcttcttct aataccttaa
      301 cagcttctat agtagttcct gctggagaac aaaccatatc ttttaattca cctggatgtt
      361 ttccagtttc aagaaccatc ttagctgacc ccattactgc ttgagatgca aatttataag
      421 cttgttgtct tggcattcca gctaatactg ctgcatctgc catagcttct ataaacataa
      481 atacatatgc aggagaagaa ccactaatac ctgtaactgt atccattaaa tattctgata
      541 ctatttctgc ctcaccaaaa gattcaaaca gagccttaat catatttaac tcttcatctg
      601 ttacattact atttttacaa agagctgaca ttccctcgcc aacaagggct ggagtattag
      661 gcattactct tactattttt ttatctgaac ctataaaact ttccagactt tctatagttt
      721 tacctgctgc tatagatact attattttat ttgcatctat taattcttta agtggagtta
      781 aagccttttc gcatatatta ggtttaagtg ctactattac cacatcagaa tttgtaacag
      841 ctacctttga atcagttgta gtatttattc caaattcttc ttttacttta tctaaggcaa
      901 cttggcttaa atctgataca gttatatctt ctgcctttac taatcctgac tgtactatgc
      961 cacctatcat tgcacttccc atattccctg aaccaataaa tcctaaagtt ttcatatcaa
     1021 atctcctttt ttctattagt tttattttaa agtagctaaa ttgactttat tttaactgct
     1081 taaaattatc ttatttttat aaaatataac cttattataa ctataataaa ttattctttt
     1141 gtatgtatta cttactaata tgtgtat
//
LOCUS       DAEPLF010000395         1164 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.398, whole genome shotgun sequence.
ACCESSION   DAEPLF010000395 DAEPLF010000000
VERSION     DAEPLF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.398"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     misc_binding    128..391
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            542..1093
                     /locus_tag="KR314_003694"
     CDS             542..1093
                     /locus_tag="KR314_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBH0329992.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          465 a          123 c          190 g          386 t
ORIGIN      
        1 aaatgtatag tataatttac tttttttata aaaaatagat tacaaaatat attttttata
       61 aaaaggtatt gacatttttt agaaaaaaga atatcattat tttaacaaca aaattcaata
      121 caataaatta aatcctatga caaggaagag tagttaaatt taggttcagt atagagagct
      181 gaggatggtg gaatcttagc aagaaaaaat ttgatgaatg gcccttagag gaataaattg
      241 aaatcatagt tttgagagta gactttatcg tcagtcgcac gttatagcga ataaggtatg
      301 taagtacctg ataatgagat gtatgaaaat tgaattttat attaattttt atataattta
      361 ggtggcaacg cggataatct ccgtcctatt agtttaggac ggagtttttt attttactga
      421 aaatttaaaa gatagaaggt ggtgagaaag atgttatgaa tttcaaaagc taaagatgat
      481 gatatagtag gttggcaaaa aaataaattg aaaattaaaa aaataggagg aagtacaaaa
      541 aatgaaaaca aatacaaaaa aattaacttt aaatgcaata cttttagcga tgggattatt
      601 aatacatcaa atcacaccag ctataggatt accaatgaag ccagatgtac cactagcaat
      661 gttatttgta atattggttt taaatagaga tgattataaa acttgtttga tagcgggtat
      721 agtaacagga atatttacag cacttacatc aagttttccg ggaggtcaaa ttcctaatgt
      781 aatagataaa actcttacag caaatatagt atttttatta atgaatatta gttataatat
      841 gccttttata aaaaaattag ctaagaaaac acaagatact gtagtagttg caataataat
      901 gccaatagga actcttgtaa gtggaactat ctttttatta gcagctcaag ctatagttgg
      961 tcttccagga gcttcattta cagctttatt cttagcagta gtattaccag ctgttttaat
     1021 aaatctagtt gcaggaatat ttttattcaa gattgttagt ctatcaataa gaagagttag
     1081 ttatcaagct taactacgaa ataatatttt actttataaa gcattataat aactaaataa
     1141 cataagaata aagtagatta taac
//
LOCUS       DAEPLF010000396         1113 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.399, whole genome shotgun sequence.
ACCESSION   DAEPLF010000396 DAEPLF010000000
VERSION     DAEPLF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.399"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            179..931
                     /gene="cwlD"
                     /locus_tag="KR314_003695"
     CDS             179..931
                     /gene="cwlD"
                     /locus_tag="KR314_003695"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBH0329993.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          493 a          111 c          169 g          340 t
ORIGIN      
        1 atacgttatc aaagtttata atataaacta agtataattt attgtatagt attgaatgtt
       61 agataaacat agtgaattaa ataggggcag aaatataata tcattacata ataatatatt
      121 aataccaata aataaagata tatcaatatt aaaaaaattt aaatgtttgt actaatatat
      181 ggaatattat agtgttttta taagtataca tatacagggt gatgttgtga gaaagtacat
      241 aaaacatata atttttagtt ttgcaatgat atgtctagta gtagtatcca tatttgaaat
      301 taaaaatatt tctgaagatg ttatcaagta tatgccagta acaaacaaaa ctataatttt
      361 agatgctggt catggaggca ttgatccagg tgcattaaat aaggataaga gtacatctga
      421 aaaggatatt aacctagcaa taacacttaa gcttagagag cttatagaat caagtggtgg
      481 ccttgtaata ttaactcgag aagatgacag tagcctttat aaagaagaaa ataataaaac
      541 tacaagacaa aaatataatg agaatttgaa aaatagaaaa gaaataatat ctaattctaa
      601 tgccaatatg tttgtctcta tacatcttaa tgcatttgaa caatcaaaat actatggagc
      661 tcaaactttt tatcctaaag acaaacaaga cagtaaagaa ttatcgaagt gtattcaaga
      721 agaacttaaa agagtagtag ataagacaaa caacagagaa gtcaaaccaa gggatgatat
      781 atatcttttg aaagaaaata atattccatc agtactgata gaatgtggtt ttttatcaaa
      841 tgaaaaagag tgtaaactct taactgatga aacatatcaa gaaaaaatag catgggcaat
      901 ctacatagga atacaaaaat atttaagttg aaataagtat aaaaataggt ctaaatatgt
      961 aaatagcttg tattaaagca gttaaaatgc attaatatag gctattttta ttttgacaaa
     1021 aaaatattta aaataaaagt taaaaagttg ttgacttaga ataatataga tgatattata
     1081 tatgagtgcc caaaaggagc accaaaataa gac
//
LOCUS       DAEPLF010000397         1096 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.400, whole genome shotgun sequence.
ACCESSION   DAEPLF010000397 DAEPLF010000000
VERSION     DAEPLF010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.400"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(97..1032)
                     /locus_tag="KR314_003696"
     CDS             complement(97..1032)
                     /locus_tag="KR314_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBH0329994.1"
                     /translation="MDKFKYAFDDKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
BASE COUNT          363 a          192 c          133 g          408 t
ORIGIN      
        1 gtataaatgt acaaattaat attttgtttt gtaaatacta aataactata gaaaggacta
       61 tatttatata accatttcta tagttattat tttgtattaa acaaacttac agccctgata
      121 agaatttcta gcctttaaag tatcatcaat ttgatttaaa atttcccatt tttttaggct
      181 ccatatagga cctactagtt catccctctt accatcacca gtgagtctat ggattatcat
      241 agtttcagga agtatttcta attggtcaca gacaagattg atatattcat cttgttccat
      301 aagagttaac ataccatttt gtaacatctt ttccatagga gtatctttta taacatgcaa
      361 tagatggatt tttataccgt ctacacccat tttaccaact gcctttgctg tttccatcat
      421 catattataa tcttcaccag gaagaccatt tataatgtgt acgacaacct ttatattttt
      481 agactttaat ttttcaacac cttcaacaaa tgttttataa tcatgacctc tatttataat
      541 tttagatgta gaatcatgaa ttgtttgaag tccaagttca acccataaat tggttctttt
      601 atttagttca gataagtatt ctacaacatc atctggcaaa cagtcaggtc ttgtggatat
      661 tgaaagacct atgacatctt caagttctaa tatagtctca tatttttctt ttaaaacttc
      721 aagaggagca tacgtatttg tataggcttg aaagtagcca atatactttg catgaggcca
      781 tttttttaac atcatttctt ttatattata aaattgactg ataagattat ctttagggtt
      841 tccagcaaaa tcacctgagc cttctttgct acagtaggta catccgccat aaccaagact
      901 tccatcaatg tttgggcagg taaatcctgc atttatagat accttaaaaa ctttttctcc
      961 aaaagtattt ctaagatagt aattccaagt atgatatctt ttatcatcaa aagcatattt
     1021 aaatttatcc aaaaaaatca cctatttcta ataaaattgt acgctgagct tgtacaaaaa
     1081 acatattacc acaaat
//
LOCUS       DAEPLF010000398         1063 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.401, whole genome shotgun sequence.
ACCESSION   DAEPLF010000398 DAEPLF010000000
VERSION     DAEPLF010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.401"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            317..964
                     /locus_tag="KR314_003697"
     CDS             317..964
                     /locus_tag="KR314_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBH0329995.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTAFKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
BASE COUNT          423 a          133 c          159 g          348 t
ORIGIN      
        1 ttataaactt ataaaattta aacaagttat tcataaaata aaataatcat catctataac
       61 ccacaacttt attttatatt aaataaaaag tattagtgat tatgttttat aagtatttct
      121 ttattcagtt atttgttaaa aactaatagt aaatcttata ctaatcgcat atttatgtta
      181 taactatatt gtaactattt tcgtaatctt aggaaaagat tacttataaa attaacctta
      241 tacaataata tatcgtataa tcttaattag cataatagtt acaatattaa aatttaaata
      301 tgggagtgat tttagtatga taagacaaac accattagag aaacgatatg acaagctagg
      361 tcctcatatt gtaacagcat tcaaaaaacg ttattttgat gcttattact gtaaaactag
      421 aaatgaagct ttgcaacaaa ttttagattt aattcctcaa aatcatgtag tatcatgggg
      481 aggatctgaa acacttaaag aaatgggcgt acaaactgct gttagagaga aaggatttaa
      541 agttattgat agagatttag caaaaactcc agaagaaaga actgaaatta tgcgtcaagc
      601 acttctatgc gatacttttt tgatgagtag caatgcaatt agtgaagatg gacaactctt
      661 taatattgat ggaaatggca atcgtgtagc tgcaatgata tttggtccaa aaaatgttat
      721 agttgtagct ggtatgaata aaattgtaaa aaacattgat gatgcaatac aaagaactag
      781 aacaattgca tctccagcag ttgttcagcg ttttcaagat gtcaatacac catgctatat
      841 taatggaagt tgtatagatt gcacaagtcc tgaaagcatt tgtgcataca tggttactac
      901 aagaatcagt cgtccagcaa aaaaaataaa ggtaatattg gtaggagaaa atcttggtct
      961 ataaaatata catacatgta aatagctcta taaataataa aatcatatga aataacttat
     1021 ttctaaaagt ggtagaataa aagcttctac aagactttca ttc
//
LOCUS       DAEPLF010000399         1063 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.402, whole genome shotgun sequence.
ACCESSION   DAEPLF010000399 DAEPLF010000000
VERSION     DAEPLF010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.402"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(91..189)
                     /locus_tag="KR314_003698"
     CDS             complement(91..189)
                     /locus_tag="KR314_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBH0329996.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFD"
     gene            368..973
                     /locus_tag="KR314_003699"
     CDS             368..973
                     /locus_tag="KR314_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0329997.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMKIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
BASE COUNT          389 a          118 c          163 g          393 t
ORIGIN      
        1 ttatcctatc tctacatata ggtttcccta tatgctgact attttagcta ctaactcatg
       61 actaaagtca cgagtgtgcg tagctatttt ttaatcaaat ccattaagac aaaataattt
      121 ttctgcctta tcaataatct cattttctct ttgtattttt tctttatctc tttttgataa
      181 ttctgacata taaattattc ctttctattg ataaaaaagt atactgatgg tatacttatg
      241 taaatataat atttgtatac ctatagtata ctttaataaa aaataaaatt aatatcaagt
      301 attataacta tttataactt tattaattaa ttatataggt aaaataccaa ataaggggaa
      361 tttatttatg aagataaaag aaaatgtatt aatgttagat agtactcgtg gttcaaattg
      421 ttttattgtg ttcgataaag aaattattct tatagacaca ggtcttcctt ttatggggaa
      481 aaatataatt aaagaattaa aaacacttgg cattaaactt acagatatta agcatatttt
      541 attaactcat catgatgttg atcatatatc taatattaaa ttattgaaag agaaaacagg
      601 agcaaaggta tgggctcatg tagaggatat accttttatt ataggagaga aggatagacc
      661 tgggtttaag aagtttattg gaaaggcaat ttccagaagg ctcattaagg atgttgaacc
      721 ttatggagag aatatgaaga ttgggaatat aaaaattatc catactcctg gtcatactcc
      781 agggcatgtc tgcatgattt ttgaagatgt attatttgca ggtgatttag tcaaaaataa
      841 gaatggaaat atagttcctt atcctaatcc atggaattgg gattataaaa agatgatgaa
      901 atctataaga gaattggata atttaaaata tgaatggata tgtatgtctc atggaactcc
      961 ttgtattaaa taataaaaat taacatggat taatagaggc atagttgctt gtatgtatga
     1021 taataattta cagcttcttt ttaatatatc atgtatattt gta
//
LOCUS       DAEPLF010000400         1050 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.403, whole genome shotgun sequence.
ACCESSION   DAEPLF010000400 DAEPLF010000000
VERSION     DAEPLF010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.403"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            131..>1050
                     /locus_tag="KR314_003700"
     CDS             131..>1050
                     /locus_tag="KR314_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBH0329998.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQA"
BASE COUNT          439 a          101 c          161 g          349 t
ORIGIN      
        1 aattatttta gcagttttgt atttatttgt atttattttg aatttattta ctaaatttgc
       61 ctataaaaag tatatataaa aatatagttc aaaatggtat aattaagtat aacagaagga
      121 ggtaatgtaa gtgagccaat acataaatat aaattctcag ttagacgccc taaaattgaa
      181 ggagttcgag aaaatatttt acgaaaatat caatgaggac gttaaagtag tgccaaaagt
      241 tactcctttt aaaggaataa ataccgattt gttgtatgtt aaagatggaa gaattttatt
      301 tataaaattt atggatacaa cagaagatat attttttatt ttagaagaag agttacttga
      361 agttatgaat gaggaatatg aacttttgaa actaaaaatg ggacaaaaga atagaaatat
      421 aagttataac tacgtttaca ttatgccata cgttgaagtt gaggaaacat atgaatttga
      481 agaatttgtt aataataata taatagataa aaataagctt caagatataa tgaataaggg
      541 ttcgctggag gagtatctta atgatgagaa tgatgaaata aacttaaatt tatttttact
      601 agacatatgt tcagaatact atataataaa tgataaactt catctaaatg aaaaatttaa
      661 aaaaatttca ttctacaatg atgactataa gtatacagca actatgatgg aagacgttca
      721 aattaaagat gttgtttcta ttaaatatgg gaatacactt attgaaggtg gttctggtat
      781 aggcaaaaca gcaattatgc tttctagggc tataaaatta gcaaaagtat atccacatca
      841 taaattagtc atatttacac atacaaaaca attgtgcaat gagcttcgag agaggataga
      901 gttattatac aaagataata acaatttaga agttcacaca tttagttctt ttatatttaa
      961 actagctaaa aaattcaatt taattgtaga ttataacatg ttaaaaaatg attacgaaaa
     1021 gacctttaat aatcttgtaa aacaagcaca
//
LOCUS       DAEPLF010000401         1050 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.404, whole genome shotgun sequence.
ACCESSION   DAEPLF010000401 DAEPLF010000000
VERSION     DAEPLF010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.404"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            133..993
                     /locus_tag="KR314_003701"
     CDS             133..993
                     /locus_tag="KR314_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0329999.1"
                     /translation="MKKQKSPILLEMLGLFLGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
BASE COUNT          386 a          127 c          173 g          364 t
ORIGIN      
        1 actatataaa aattcaaaaa tattaagaat aatgaaatac ttgaaattgc ttatatcaag
       61 aaattttata taatataaac tggtcaaata tgttataata ttaaattgtg acaagttcat
      121 ggaggtgttt atatgaaaaa acaaaagtcg ccaattttac ttgaaatgct tggactattt
      181 ttgggttgta tatcaatgag tattggtata aatatgtttt taaaaccaca tacaattgct
      241 cctggaggac ttagtggtct atcattagta ctaaacaagg ttacaggact tccagtatca
      301 gctatcatgt taattatagg tgtcccatta gtaatacttg cttttagaat tatgggtaca
      361 aaaaattcat taaaaacttt atttggaaca gttgtatttt cagctatagt tcaattaaca
      421 gacccattat caaaactaag atttactaat gacttattat tgtcttctat tgcaggagga
      481 atcctagttg gtataggttt aggaataatg tttaaatcag atgcttctac tggaggaaca
      541 gacttaattg cactcatact tagtaagaaa ttcccaggaa taaaagctac aaaattcatg
      601 tcatgcctag atggaatggt tgttatatct tctggtatag taaatcgtag tttagaaact
      661 gctctttact ctggtatagc actttgtgta ttaataaaaa tagcagatat gataatggaa
      721 ggatttgacc attcaaaagc attttttatt atctctgatg agcctgaaag tttaagacaa
      781 gctataactg aagaacttga tagaggtctt actattcttg atggtaaagg tggatacact
      841 agagaaaata aagaagttct attagtcgta gtttctaaaa aacaagaact ttatctaaag
      901 agattagtta aaagaactga ccctactgca tttgttattg ttacagatgt gcatgaggtt
      961 cttggagaag gatttaaaaa tttagagaac taataaaaat taatatttta agtatatata
     1021 ctattttaat taaaatacga tttattcaaa
//
LOCUS       DAEPLF010000402         1040 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.405, whole genome shotgun sequence.
ACCESSION   DAEPLF010000402 DAEPLF010000000
VERSION     DAEPLF010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.405"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            33..1040
                     /locus_tag="KR314_003702"
                     /pseudo
     CDS             33..1040
                     /locus_tag="KR314_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612258.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          422 a          118 c          195 g          305 t
ORIGIN      
        1 atgatgttaa aacaactaca gatattcaag atatgctaaa agatttattt gcgagtacaa
       61 ttcatcaaat gttagaagcg gagcttgacg accatttagg ttatgataga tatggcaata
      121 agaataaaaa tactaaaaac tctagaaatg gatatagaaa taaaaatgtt aaatcagatt
      181 ttggagaaat aactttaaat attccaagag accgaaatgg agattttgaa ccaaaggtaa
      241 ttagtaatta taaaaatgat atatcaggaa tagagaacca agtgattgga atgtactcaa
      301 aaggcatgtc tactcgagat atatcaagcc accttaagag tatttacgga attgatatat
      361 ctcattctct agtagctaaa attactgata gagttctacc tttagctcat gaatggcaaa
      421 acagacctct tgatatggtt tatccaattg tatttatgga tgctatacat tataaagtta
      481 gaagtgaagg tagaataata aacaaagctg catatatagc cataggtgtt aacttagaag
      541 gtataaagga ggtcttagga atatggattg gagaaaatga aagttcaaag tattggctaa
      601 aggtgcttac agatgtcaaa aacagaggag ttaaggatat attaataact tctgtagatg
      661 gattagttgg attttctgat gcaattaaag cagtatttcc tgatactgaa atacaaagat
      721 gtgttgtaca tcaaattaga aatactttaa attatgtatc atataaaaat agaaaagaat
      781 gtgcaaaaga ttaaaagaaa gtatatgctg caattacaga ggaagtagca ttaagtgagc
      841 tatcgctctt agaggaaaaa tggggtaatc aatatgcgat agcattaaga agttggcgaa
      901 gcaattgaaa tgagctagca acatacttta aatatcctca agagataaga acattgatat
      961 atactacaaa ttcaatggaa agttacaata gattattaag aaaagttact aaatcgaagt
     1021 ctatttttcc tttagatgat
//
LOCUS       DAEPLF010000403         1024 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.406, whole genome shotgun sequence.
ACCESSION   DAEPLF010000403 DAEPLF010000000
VERSION     DAEPLF010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.406"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            78..194
                     /gene="rrf"
                     /locus_tag="KR314_003703"
     rRNA            78..194
                     /gene="rrf"
                     /locus_tag="KR314_003703"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            202..276
                     /locus_tag="KR314_003704"
     tRNA            202..276
                     /locus_tag="KR314_003704"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:234..236,aa:Asn,seq:gtt)
     gene            282..367
                     /locus_tag="KR314_003705"
     tRNA            282..367
                     /locus_tag="KR314_003705"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Leu,seq:taa)
     gene            383..458
                     /locus_tag="KR314_003706"
     tRNA            383..458
                     /locus_tag="KR314_003706"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:416..418,aa:Met,seq:cat)
     gene            466..540
                     /locus_tag="KR314_003707"
     tRNA            466..540
                     /locus_tag="KR314_003707"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:499..501,aa:Glu,seq:ttc)
     gene            555..628
                     /locus_tag="KR314_003708"
     tRNA            555..628
                     /locus_tag="KR314_003708"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:587..589,aa:Gly,seq:tcc)
     gene            634..709
                     /locus_tag="KR314_003709"
     tRNA            634..709
                     /locus_tag="KR314_003709"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:667..669,aa:Val,seq:tac)
     gene            715..791
                     /locus_tag="KR314_003710"
     tRNA            715..791
                     /locus_tag="KR314_003710"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:749..751,aa:Asp,seq:gtc)
     gene            802..876
                     /locus_tag="KR314_003711"
     tRNA            802..876
                     /locus_tag="KR314_003711"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:834..836,aa:Gly,seq:gcc)
     gene            886..959
                     /locus_tag="KR314_003712"
     tRNA            886..959
                     /locus_tag="KR314_003712"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:920..922,aa:Arg,seq:tct)
BASE COUNT          272 a          243 c          263 g          246 t
ORIGIN      
        1 taactctaaa gaaactagta ttgctaggtt ctataaactt attaaaaact taatagaaat
       61 attaagcata aagattatgt ggttacaata gcagagagga tacacctgtt cccattccga
      121 acacagaagt taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta
      181 ggacgtagcc acgttaaatg ttccaaggta gctcaacggt ggagcaaccg gctgttaacc
      241 ggtaggctgt gggttcgagc cccacccttg gagccaaaaa agccgaagtg gcggaactgg
      301 cagacgcaca ggacttaaaa tcctgcggga cttacctctc gtaccggttc gattccggtc
      361 ttcggcactt ataaaacaat atcgcggggt ggagcagttg gcagctcgtc gggctcataa
      421 cccgaaggtc gtaggttcaa gtcctgcctc cgcaaccatt aaaacggctc gttggtcaag
      481 aggttaagac accgcccttt cacggcggta acaggggttc aattccccta cgagtcacca
      541 attcaattaa atatgcgggt gtagctcaat ggtagagttc tggccttcca agccagctgt
      601 gagggttcga tccccttcac ccgctccaaa aatgggacca tagctcagct gggagagcac
      661 ctgccttaca agcagggggt cacaggttcg agccctgttg gtcccaccat tcgcggcctg
      721 gtagttcagt tggttagaat gccagcctgt cacgctggag gtcgagggtt cgagcccctt
      781 ccaggtcgcc aaattaaata agcgggaata gttcagtggt agagcgcaac cttgccaagg
      841 ttgaagtcgc gagttcgaat ctcgtttccc gctccataat aacttgggtg catagctcag
      901 ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct gtgcgctcat
      961 taagaaaagt ttactataaa ttatagtaaa cttttatatt gtttaaaaag acacaggaaa
     1021 catg
//
LOCUS       DAEPLF010000404          980 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.408, whole genome shotgun sequence.
ACCESSION   DAEPLF010000404 DAEPLF010000000
VERSION     DAEPLF010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.408"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(1..295)
                     /locus_tag="KR314_003713"
                     /pseudo
     CDS             complement(1..295)
                     /locus_tag="KR314_003713"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            355..848
                     /locus_tag="KR314_003714"
                     /pseudo
     CDS             355..848
                     /locus_tag="KR314_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          386 a          149 c          111 g          334 t
ORIGIN      
        1 tctctattat tttaactaat tgttcatatg tatcaaattt ccttaaatag tacatttcca
       61 cctttaaaat tccccaaatt ccttccatag gagcattgtc aatacaacga ccagctcttg
      121 acatactttg tatcatacct tgtgctaaaa gtttcgattc aaacacctta ctagtatact
      181 gaaaacctct atcactatga aaaacaggtt ttgcatttgg atgtagtttt attactttat
      241 caaaagtttc gaataccaag ttattattat tcgattttcc aataacatat gaaattaata
      301 gatataacgc cttctatttt acagtcttat ataaataaca tatacaatga gcacatgtta
      361 aattcaataa aaaaccaagt cgattttata aaatcagtat tgaaagaatc ttatagatta
      421 aaagaaatat cagaaaatat ttgtgatttt gttacaaccc caatcaaaaa aaatagttca
      481 acttctaagt tttatacaaa gcaagaaact caattattat tagaaaaatc aaaaaatatt
      541 cctataggta taccaatttt tttgatacta actcttggat tacgttttgg tgaagctgta
      601 tcacttatat ggtcagatgt agaccttgat aaaaaatctc acatgtaaat caaacattag
      661 tttatgtata caataaaata atcttcaaag accctaaaat ccctagatcc aaaagaaaat
      721 tatttgctcc tgatgaactt ataagtttgc ttaaagggca aaaattaaaa caaaataagt
      781 taaaattaca gggtatattg aaaaatgaat ttgatttgat atgcttaaat aaaagattca
      841 acccgtgagt tcaaccaact tttgttgttc attttaaaaa actattaaga gataataatc
      901 ctagagatat aaaagtacat gaattaatac atgcaaatgc gactttgata ttactttcag
      961 gtactaacat aaaagctatt
//
LOCUS       DAEPLF010000405          976 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.409, whole genome shotgun sequence.
ACCESSION   DAEPLF010000405 DAEPLF010000000
VERSION     DAEPLF010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.409"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(39..953)
                     /locus_tag="KR314_003715"
     CDS             complement(39..953)
                     /locus_tag="KR314_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBH0330000.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKDITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
BASE COUNT          305 a          150 c          126 g          395 t
ORIGIN      
        1 tctaaatatg caaagattat tttaaaagta atttttaatt acatgttttt ataactctcc
       61 aatattctaa tataatgatt tgcctctctt aaaacatggt ctgctaaaag tggtaatatt
      121 atagatttta tcttacactg ttctattcct gatgctcctg cttctttaaa atctttaaac
      181 tcaacagttt catttagagt ttcttctgta atgttcttga tattagaatc agtcatttcg
      241 tttgtttttt caattaattc attaaatttt atagcaaaat catttgaagt atttattagt
      301 tcaccttctg aggggtctag taatcctctc ataaacagag catgttccat cataatatgg
      361 tcccagaata attctatttc tttagcgttt tttgactcaa tatctatttt attttcaagg
      421 tcaactacat aagaacgata taaatttgct tcatgtatta tatgttcaag aagtagaggg
      481 tagtttgatg taaatatagt acatgatagt acaccatcta agactctttc tttaaaatta
      541 ataagcccat caagtaatct tattgcatca gagttaagtt gagctacata gttcactaag
      601 tcttgaccaa cttgtgggtt tacaccactt tgtagattta attctcttgt agtgatgtct
      661 ttgtttattt ctatccctgt aaactcttct gttttttgtt ctgcaactga tgtgagagta
      721 gttacaagct cttctgaata taatatatca ggtctaatta taccattact agcactaaca
      781 gtgtatgata ataaatcttc aaattgcttt ttatagtggt cagcttccat agcaagatta
      841 taatttttat ttgtgaatcc tgcttctaaa aaaagagcat gctctttcat aattcttgaa
      901 aaaaataaat gtaattctag tgataaaata acatattttt gattatctat cataatttcc
      961 tccctttaga aagtat
//
LOCUS       DAEPLF010000406          950 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.410, whole genome shotgun sequence.
ACCESSION   DAEPLF010000406 DAEPLF010000000
VERSION     DAEPLF010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.410"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            528..>950
                     /locus_tag="KR314_003716"
     CDS             528..>950
                     /locus_tag="KR314_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0330001.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFC
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPR"
BASE COUNT          378 a           94 c          152 g          326 t
ORIGIN      
        1 ttttgaatga taattaagtg cttgttggta aggttatggc tgtaaaaatc ataactttat
       61 ttttatattt gttccatttt cataaaaatt attatccctt taagaaaatt tcttaatcct
      121 cttgaaatat ttccttttta gtctaaagtt aaatttcttt cttcaaaatg aatatttaaa
      181 tgaactaaaa tttaagtaca ttttctaatc ttatgagtct atttttcata gataattgcc
      241 ctataataga agaatctatg ttggggctat gtggtgacaa taacagcatt gtcagtgatt
      301 aattaatgct ttataaatga ttaattagtg ttttatgtta tttttggact taccctctat
      361 acatagatta atttatttaa ctttagatat agagagaatg tcaagtacat acacagacat
      421 tagagtttaa gaaagtgttt atggagcaag aacttttaaa agaagtagtg acttagttta
      481 atttcaagaa atgataaata caaaagagga aaaagagagg tgttgaaatg atagaaaaag
      541 tagaagaaaa atctaagatt attaaaacat caaaagaata taagcgaact ataattgaag
      601 aaaaagataa agaatggacc aaaaaattaa atgatatact tagtagaagt agtttttgta
      661 atgatgaatt agataaaatc aaggcgttaa ttccaaaaga gattttgact aatgaaaatg
      721 ttggagaagt tgtgactgaa gatggatatg ctaagcccga gtatgatgag gtatttaaat
      781 cattgttcac tttaaataat gaatatgatt tacttgtaaa ttttataaat gatattctaa
      841 aagatgctcc atatgctaac aggaatatca aaccgttcac tcaaataaag agaataatta
      901 gagttgaaac tgatccaaca attaattaca ttggagagaa aagacctaga
//
LOCUS       DAEPLF010000407          944 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.411, whole genome shotgun sequence.
ACCESSION   DAEPLF010000407 DAEPLF010000000
VERSION     DAEPLF010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.411"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            57..425
                     /locus_tag="KR314_003717"
     CDS             57..425
                     /locus_tag="KR314_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0330002.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            428..799
                     /locus_tag="KR314_003718"
     CDS             428..799
                     /locus_tag="KR314_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBH0330003.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
BASE COUNT          368 a           92 c          166 g          318 t
ORIGIN      
        1 ctaattaaga gtactataat ttaattaagt aattacttaa tcaaaacgag gtacttatgg
       61 aaaacttggt taaaatattt aaagcattga gtgatgaaac tagacttaac atacttatat
      121 tagtatccaa gagaaatata tgtcaaaagg ggatttctaa atatctaggg atttcagatt
      181 ctgctgtttc acaacatata aaaatattaa aagatgttgg gataataaca ggatataaag
      241 aaggatacta tgttttatat catattaata aagaatcatt taatatatgt gtagatttta
      301 taaattctat gttaagtaat tctagtgaaa gctttataga tgtatttgat gtaaatacaa
      361 tacatctagg atgcagtaaa gaatgtaaat ctataaaaaa atgttgtaag agaagggaga
      421 aataataatg aaagtatgta taccagtaga ggagaataaa ggattggata gtaaaccata
      481 tggacatttt ggttctgctc caatttttgt tgtgtgtgat ttagaaagtg gagaagttaa
      541 atctttagat aatggagatt tagatcatga acatggaaaa tgtcaaccat taaaagcatt
      601 gtcagggaca gctgtagatg cagtagttgt tggaggaata ggacaaggag ctataattaa
      661 attaaatgga atgggaataa aagtttacag agcagaaggt gatactataa gtgctaattt
      721 agacttactt aaaaatggaa aattagtaga gtttccatca gaccatactt gctcacatga
      781 cggatgtgga catcattaaa aaaaacagct gatgaaatat atacttcatc agctattttt
      841 tcttttaata aatacgattt tattctttta aaaccaaaat ttagattttt atgttttttg
      901 tagtatcctt aataatattt caagtttaca agtttactac tttt
//
LOCUS       DAEPLF010000408          931 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.412, whole genome shotgun sequence.
ACCESSION   DAEPLF010000408 DAEPLF010000000
VERSION     DAEPLF010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.412"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            82..780
                     /locus_tag="KR314_003719"
     CDS             82..780
                     /locus_tag="KR314_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0330004.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHEYQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTILENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          364 a          112 c          136 g          319 t
ORIGIN      
        1 attaaattat atattacaac attattttat agtttgatta attcaacaat cactaatata
       61 tcaaactgaa aggagatttt tatggaaaaa aaatatttcg aaattcatgg agttccttca
      121 atcctttggg gaaatccttc aagtaaatta tatatttaca tacatggaca atgcggcaat
      181 aaagaggagg ctgaagcttt tgctaatatt gcagttccac atgagtatca agttttaagt
      241 gttgatttgc ctgaacatgg agaacgtaaa agagaaaaaa attcatttaa cccatggcat
      301 gttgttccag aattagtatc tattatggaa tatgcaaagt gccactggaa tcaaatttcg
      361 ttatatgcaa atagcattgg agcatggttt agtatgttaa gctttgaaaa agagaactta
      421 agagagtgta tttttgtatc tccaattcta gatatgcaac agctcatttc aaatatgatg
      481 ctttgggcaa atgtatcaga agaacagctt aaacatgaac taattatccc aacttcattt
      541 gggcatactc tttcatggga atatctacaa tatgtaaaac aacatcctat cactcaatgg
      601 aatgtaccaa caaaaatatt atatggtaaa catgatgaac ttactgaaat cggtgttgta
      661 gagaaatttg tagaaaagtt taattgcaac cttactattc tggaaaatgg caaacattgg
      721 tttaacacac cacaagaatt aaattcatta tatgaatggt taaatacttt agttaaataa
      781 aaaataatta tattattaat atgattaaat tatttataaa tgttatttaa ttaattttat
      841 tttagcatta ttaaaataac tacataaaga ataaattagt tattattaaa gaaattaaaa
      901 gtatatagag tattaataat tttttaataa t
//
LOCUS       DAEPLF010000409          929 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.413, whole genome shotgun sequence.
ACCESSION   DAEPLF010000409 DAEPLF010000000
VERSION     DAEPLF010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.413"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            110..622
                     /locus_tag="KR314_003720"
     CDS             110..622
                     /locus_tag="KR314_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0330005.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEDYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKAKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
BASE COUNT          425 a           67 c          179 g          258 t
ORIGIN      
        1 caaaaagttt ggttacataa tagtaatttt agttacaaga atgaaactgt aaaacttagg
       61 attttcttta tataatttca ttgagaacat ttaatttagg aggtaaggta tgaaagtaaa
      121 aaaatttaaa agtggtatag taatggcatt aacagtaatg gcaattggag catcattgac
      181 aaatgtatca gctatggaat taaacaagag taaagagttt gaaactaaat acaatgaaat
      241 gaagagggat tttcaagagg attatggaaa agaagacaaa tttaatataa cattggatga
      301 tgaatttaca aaagaagaag taaatggagc taaagggatt ataaagacaa ctaataaaat
      361 gaataatgag gttgtagtat taaattattt tgatgaaatg aaatctggga ttgaatttga
      421 gttgaaggca aaagcagaaa aaaatgcaat tttgagagat gaatttcaac aaggttatgg
      481 aaaggaagat aaattcaaag ttgtgttaga tgatgaattt acaaaagaag aggtaaatgg
      541 agctaagggc attataaaga ctacagataa agagacaggt gaggttatcg tatataatta
      601 ctttgatgag cttgaaaaat aatgcttaaa gattactttg attgaatgaa aaggtaattg
      661 tgttgagaat attaaatgac tgaataaaaa cagttgtatc aaaaacaaac aataatagaa
      721 tgtaagaatg attgtgtcga aaatattaaa tgactgaata aaaaatagtt gcgtcaaaaa
      781 caaacaataa tagaatgtaa gaatgattgt atcaagaata taaaatgatt caacaaaaaa
      841 cgattgtgtt aaaaacaaac aacaatagaa tgtaagaatg attgtatcaa gaatataaaa
      901 tgactgaata aaaacgattg cgtcaaaaa
//
LOCUS       DAEPLF010000410          918 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.414, whole genome shotgun sequence.
ACCESSION   DAEPLF010000410 DAEPLF010000000
VERSION     DAEPLF010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.414"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(58..258)
                     /locus_tag="KR314_003721"
     CDS             complement(58..258)
                     /locus_tag="KR314_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH0330006.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(483..659)
                     /locus_tag="KR314_003722"
     CDS             complement(483..659)
                     /locus_tag="KR314_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0330007.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(713..802)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          345 a          148 c           84 g          341 t
ORIGIN      
        1 caaaatgcgt aaataaaaaa aagactctta aaagagtctt tccgaaaaat ctatatatta
       61 taatatagtt atattttctg cttgaggacc tctagcgcct ttaactatat caaagcttac
      121 tttttgacct tcttctaatg atttaaatcc tgaagtttgt atagctgaga aatgagcaaa
      181 tacgtcatct ccactttcca ttgatataaa tccaaatcct ttttcattat taaaccattt
      241 tactattcca ttattcatta aaaatactcc tattaaccta tatcaagata tacttgattt
      301 acttgtgtat taccacaaat actattatat catagattaa cataaatgtc tatttaatta
      361 taatttttag ttaacatttt tatttattct taatatgaaa ataaatttaa acattttata
      421 ttaccaatat attaatgtaa atgtagataa aactaaaaag ggttttctag aagaaaatct
      481 gtctaattat acttacttac caataccatc acttgatgca agcaagattc cacaactttt
      541 tctccgtact ctaatataaa atcatctaac tgtgtatcta taaaatcaca ataatcatat
      601 gaactaccaa tataagcagt tagcatatgt gtacatttat ccattttatc tctaaacata
      661 atgtatttat ccccctaaaa aactttcatg aaaaacaaaa agaaaatatc tttttggcaa
      721 ctggctgaca taacttattt tataagcgtt agtccctata gctttgcgtc actaaatttc
      781 tctagttttg ccgatttagt tttattctac agattaataa tacaatagaa ttagctatta
      841 ttcaacatat ctagctgaaa aattaataaa tttatatatg aacgttaaat tatttgaaaa
      901 aataaaaagc ccctctgt
//
LOCUS       DAEPLF010000411          897 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.415, whole genome shotgun sequence.
ACCESSION   DAEPLF010000411 DAEPLF010000000
VERSION     DAEPLF010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.415"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(103..813)
                     /locus_tag="KR314_003723"
     CDS             complement(103..813)
                     /locus_tag="KR314_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0330008.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELEKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVHIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLEP"
BASE COUNT          299 a          124 c          108 g          366 t
ORIGIN      
        1 attaagcttc aaaaaattaa atttgaaaaa acaaaactct aaaaactgag ctttaaaaaa
       61 ttaagtttca aaaataaaat tctagagatt gaacttcaaa aattaaggtt ctaacatata
      121 ccttgtaaat tttcctacaa cctcttgata ttttttacgg cttataggaa ttttttcatt
      181 ctgaactttg gcataagttt gagttatact atctacatgg tttagattga caatatacct
      241 ttgatgcgta cgtataaaat tagagggaag aacagtttcc atatcattta attttccgta
      301 gtattctaat gttttatcaa aggtaactac agttaatttt cttagctcgc ttttaaagta
      361 gaggatatct tttgtattaa ttttacaaag attagttcca atatgaactg taataaagtt
      421 ttctttccat tgttctaatt tttttagtgc ttcttttaat actttacata ttttttcagt
      481 tttataaggt tttaagatgt agttcaaagc tcctacatca tacccatgaa atacatagtc
      541 agaaaaacct gtgacaaaga tgattatagt atcttttgaa tgttttcgta tagtttttgc
      601 tatgtcaacg ccattacttt tttcaagttc aatatctaaa aaaataatat ctatgtaata
      661 attttcgtgt tctagattgt atactagact ttctccattt gaatattctt ttatttggta
      721 ggggtagccc cacaaatcca tttctcttgt aatgatttta ttcagcatgt ttctttgttg
      781 tttttcatca tcacaaatac aaatcgaagt caatttttta tctcctttca agttttatat
      841 ttatatagtt aagtatataa tatattttgc tacctagata tataaaatga aataaca
//
LOCUS       DAEPLF010000412          883 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.416, whole genome shotgun sequence.
ACCESSION   DAEPLF010000412 DAEPLF010000000
VERSION     DAEPLF010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.416"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..240
                     /locus_tag="KR314_003724"
     CDS             <1..240
                     /locus_tag="KR314_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0330009.1"
                     /translation="FIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNI
                     REWICPSCNEIHDRDINASINILKEGLRLITIQNK"
     gene            305..766
                     /locus_tag="KR314_003725"
     CDS             305..766
                     /locus_tag="KR314_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0330010.1"
                     /translation="MQNSVKQEPCDFSHGRFRFGGKIIRRNEKTKFDWLSRDKEQVEK
                     YINDPFCGVVCSCGFFYDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGV
                     LRLRDRYIKLGVKDVTCKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
BASE COUNT          366 a           72 c          183 g          262 t
ORIGIN      
        1 tttattcgtc aattagaata caaggctaat tggcatggta gacaaattgt aaaggtaggt
       61 aaattctttg caagttcaca aatatgcaat aaatgtgggt ataaaaacga ggaagtgaag
      121 gacttaaata taagagaatg gatttgtcct agttgtaatg aaattcacga tagagatata
      181 aatgcaagta taaatatatt aaaagaggga ttaagactaa taacaattca aaataaataa
      241 cttatgtaag aaccgtagga actacgggga tagcctgtgg agaattagta agacatattt
      301 tagtatgcaa aattctgtga agcaggaacc ctgtgacttt agtcatggga ggttcagatt
      361 tggtggcaaa ataataagaa gaaatgaaaa gactaagttt gactggttaa gtagagataa
      421 agaacaagtt gaaaaatata taaatgaccc attttgtgga gtagtgtgta gttgtggctt
      481 tttttatgat ttagttcaag ggttaaaaga aattgaggat aaagaaaact taaaaaaagt
      541 gccacttgat atacctattt acataatatc tggagataaa gaccccatag gaaaaaatgg
      601 taaaggagtc ttaagactaa gggatagata tataaaatta ggagtaaaag atgtaacttg
      661 caaactttac aaagatggaa ggcatgaatt gttgaatgaa ataaatagag aagaagtttt
      721 tgaggatata atttgttggt taaacaataa aatagaaata ttgtaataaa aaaatataat
      781 gatatatttg ctttaaattt atttatatat aatatttgaa aagtggatta tgtatatgca
      841 aagtagaaaa gagagagcct agaagtgatt ttttgctata atg
//
LOCUS       DAEPLF010000413          852 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.417, whole genome shotgun sequence.
ACCESSION   DAEPLF010000413 DAEPLF010000000
VERSION     DAEPLF010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.417"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(219..713)
                     /locus_tag="KR314_003726"
     CDS             complement(219..713)
                     /locus_tag="KR314_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0330011.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIIALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKNDYGNLIDITNDQNKINIVIKLLNN
                     KSNT"
BASE COUNT          278 a          130 c           80 g          364 t
ORIGIN      
        1 tgtataaact atcaagaata ttactcaaca caatttaaaa acaacttaaa taggtatttt
       61 tagcaacata aattatgtga tataataaaa aatagaagtg tatgccaata cacttctgta
      121 gttataacta agcattctat tcttggggag tgcttttttc atttctccaa acaagattgt
      181 ttatttcatc agtaattatt aaatcaaatt tattgtattt aagtattcga tttattattt
      241 aaaagtttta ttacaatatt tattttattt tgatcgttcg ttatgtctat taaatttcca
      301 taatcatttt ttaatttatc tagaccactt ttgcttattt ctccttctgg aagtatgctt
      361 aattctagat atttttcatg accaggagat gacattaaca gatatgaacc attataaggc
      421 atccctaaat tttctatttt atctgctaat gggtcatctt taaaaccccc atttattatt
      481 atttttccat ctttctttac ccatgatatc attcctccat cagtatactt ggattcatca
      541 gcatagtaac taagagatgc actttttatt gcacttatat cagcttcaag tttagctatc
      601 tttgcttttt ctatattttt aaataatgct ggaagtgcta ttattgccaa tattcctatt
      661 atagcaatta ctactaataa ttccactaga gtaaatcctt ttttattttt catttatttc
      721 ccctccatat aaaattaata atatataaat ttttattttg ttgatttcta ctttctatat
      781 tttaccatat atagacaaaa aaatcacttt ctatgacaaa tatactatta ttttacaatt
      841 tatagaaatg at
//
LOCUS       DAEPLF010000414          752 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.418, whole genome shotgun sequence.
ACCESSION   DAEPLF010000414 DAEPLF010000000
VERSION     DAEPLF010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.418"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..>752
                     /gene="tuf"
                     /locus_tag="KR314_003727"
     CDS             <1..>752
                     /gene="tuf"
                     /locus_tag="KR314_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBH0330012.1"
                     /translation="ELLELVEMEVRDLLTEYDFPGDDTPIVRGSALMALEDPKSEWGD
                     KIVELFEQIDEYIPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEVEL
                     VGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAH
                     TKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTME
                     VDLINSIVVEEGLRFSIREGGRTVASGVVATI"
BASE COUNT          310 a           94 c          187 g          161 t
ORIGIN      
        1 gagttattag agttagtaga gatggaagta agagatttat taacagaata tgatttccca
       61 ggagatgaca ctccaatagt aagaggttca gcattaatgg cattagaaga tccaaagagc
      121 gagtggggag ataagatagt agaattattc gagcaaatag atgagtatat accagctcca
      181 gagagagata cagataagcc attcttaatg ccagtagagg atgtattctc aatcacagga
      241 agaggaacag ttgcaacagg aagagtggaa agaggagtac taaaagtaca agacgaagta
      301 gaattagtag gattaacaga agcaccaaga aaagtagtag taacaggagt agagatgttc
      361 agaaaattat tagaccaagc acaagcaggg gataatatag gagcattatt aagaggagta
      421 caaagaaacg agatagaaag aggacaagta ctagcaaaga ctggatcagt aaaggcacac
      481 acaaagttta cagcagaagt atatgtactt aaaaaagaag agggtggaag acatacacca
      541 ttctttgatg gatatagacc acaattctat ttcagaacaa cagacgtaac aggagcttgt
      601 aagttaccag aaggaataga gatggtaatg cctggagata acgtaacaat ggaagtagac
      661 ttaataaact caatagttgt agaagaggga ttaagattct caataagaga aggtggaaga
      721 acagtagctt caggagttgt tgctacaata at
//
LOCUS       DAEPLF010000415          742 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.419, whole genome shotgun sequence.
ACCESSION   DAEPLF010000415 DAEPLF010000000
VERSION     DAEPLF010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.419"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            188..694
                     /locus_tag="KR314_003728"
     CDS             188..694
                     /locus_tag="KR314_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBH0330013.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          299 a           72 c          129 g          242 t
ORIGIN      
        1 atatatttta tctatatttt acacttataa aatatgaaaa ctacttattg cgaaagtaat
       61 tgatttgttg tattataatt tacatgaatt atgtaggaca atcaaaagtc agtattataa
      121 gagtacatat attcaaattt ttaacaatta atttatataa ttaaaaaatg aagggtagga
      181 tgaagtcatg ggttttagag aagtaaaaat tgaggaacta cagtttaatc catttactaa
      241 gattggcaaa gaatggttac ttataacagc tggtgattca gaaaagttta acacaatgac
      301 tgctagctgg ggtggtgttg gagtctattg gggtaaaaat gttgttacaa cttacataag
      361 accacagaga tatacaaaag aatttgtaga tagtaatgat acatttacag ttgccttctt
      421 tgatgaaacg tatagagagg ctttaaatat ttgtggtact atttctggta gagatataaa
      481 taaaatagaa aaagcaggac ttacaccata ttttgtggat gatacggtag cttttgaaga
      541 agctaatatg attatagtat gtaaaaagct atatcatggt aatatgccac cagaaaactt
      601 tgatgctaaa gaaaatgata aaaaatggta tccagagaaa gactatcata ctatgtacat
      661 ttcagaaatt ataaaagttc ttgtaaaaga ataattataa taaaaaatat atgaaaagaa
      721 cttattataa gtaaatgact gt
//
LOCUS       DAEPLF010000416          735 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.420, whole genome shotgun sequence.
ACCESSION   DAEPLF010000416 DAEPLF010000000
VERSION     DAEPLF010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.420"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            142..480
                     /locus_tag="KR314_003729"
     CDS             142..480
                     /locus_tag="KR314_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0330014.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            502..>735
                     /locus_tag="KR314_003730"
     CDS             502..>735
                     /locus_tag="KR314_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0330015.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDI"
BASE COUNT          333 a           79 c          111 g          212 t
ORIGIN      
        1 gtagatacag actatacaaa aataaatagc acttatttca gtataaaaac atatatagtc
       61 tatatttaga ctaaaaaagt ataaatagtc tatataaaac aacttaaact atgaatataa
      121 tctaaagtgg agggataata attggcaaat ataggaaaaa taataggaga tagaataaaa
      181 gtctatagaa caaaattggg atatagtcaa gagtttctag cagaaaaggc aggtttacat
      241 ccaacatata ttggtcagat tgaaagagct gagacaaata catctatcaa tattataatg
      301 aaaattgcta ctgctttaga cataccactt gaattgttat ttgcaaatat tattacaaca
      361 gaaggcgttg ataactctgt acctttagaa tgttatgaaa tgattaataa attaactgaa
      421 aaagaacaaa ttgcaatgct agagttaata aagtgcatca ttaaatacaa agaaatataa
      481 gtgagggaaa tagtaacaat aatgaattat ataggtaaaa gacttaaaga agaacgaaaa
      541 aaagcaaatt taacttcaaa agaatttgca aatatggttg gagtatcgcc ttggtatata
      601 actcaaatcg aaagtggtaa aaagaatcca agtcttaaaa cattcataaa atttgtaaat
      661 atattaaata tttctgcaga tgttcttata aaagatatta cttccactgg aaaaacatat
      721 ttagaaaatg atata
//
LOCUS       DAEPLF010000417          728 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.421, whole genome shotgun sequence.
ACCESSION   DAEPLF010000417 DAEPLF010000000
VERSION     DAEPLF010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.421"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..>728
                     /locus_tag="KR314_003731"
     rRNA            <1..>728
                     /locus_tag="KR314_003731"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          195 a          157 c          236 g          140 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtactag gtgtcggggg ttaccccctt
      601 cggtgccgca gctaacgcat taagtactcc gcctgggaag tacgctcgca agagtgaaac
      661 tcaaaggaat tgacggggac ccgcacaagt agcggagcat gtggtttaat tcgaagcaac
      721 gcgaagaa
//
LOCUS       DAEPLF010000418          724 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.422, whole genome shotgun sequence.
ACCESSION   DAEPLF010000418 DAEPLF010000000
VERSION     DAEPLF010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.422"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(106..483)
                     /locus_tag="KR314_003732"
     CDS             complement(106..483)
                     /locus_tag="KR314_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0330016.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDATVTSKDVIEVMEQKYGWKQTTT
                     LTLLSRLVKRGFLNAQKIDRYTHYTVIVKHKDYLSFETKDFLSNIHDDSLKSLISALH
                     DDENIGKDTLDFLESYFDNLKNE"
BASE COUNT          244 a          106 c           68 g          306 t
ORIGIN      
        1 ctaaacctct taaaaaaata tcttttcaaa aataataata gacaaatacc aaaactacat
       61 acaatagttg tctttataag attgtcaaaa acatatagca tataattatt cattctttaa
      121 attatcaaaa taactttcta aaaaatctag cgtgtcttta cctatatttt catcatcatg
      181 taatgctgaa attaagcttt tcaaagaatc atcatgtata ttacttagga aatcctttgt
      241 ctcaaaactt aaataatctt tgtgttttac tattactgtg taatgtgtat atctgtctat
      301 tttttgagca tttaagaatc ctctttttac taacctcgat aaaagtgtta gtgtagtggt
      361 ttgtttccaa ccatattttt gttccataac ttcaataaca tcttttgatg tcactgtagc
      421 atctactttc caaataaatt tcattacttt tagttctgct tgtggtattt ttcttatcat
      481 catttacacc tccctcaaaa ctcttgctac tttaagtcta caatatgtga attttttagt
      541 caaacgcttt atgaaaaaat gtggattttt gtacttttta ttaattttct aattttttaa
      601 aatactaaaa gttagatttt aaaatatagt tgttcaagta ttttgctttt tacatattta
      661 attataatag tgcagaatgt tatttccaca ttaaaattat ttaataaaaa ttcctatatt
      721 ttca
//
LOCUS       DAEPLF010000419          716 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.424, whole genome shotgun sequence.
ACCESSION   DAEPLF010000419 DAEPLF010000000
VERSION     DAEPLF010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.424"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            101..676
                     /locus_tag="KR314_003733"
     CDS             101..676
                     /locus_tag="KR314_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBH0330017.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPETLEQFHTNDYNLAKQYAMNTELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
BASE COUNT          276 a           81 c          123 g          236 t
ORIGIN      
        1 aaaattagga ggtcttgttt tgaaaaaaat acttgattat attccacctt ttcataactt
       61 tctattaatt tagtaatctt atagaaagga agttgcattt atgaaaaaaa ttacttggga
      121 ggtggaatat atctcccagt tacctgtaat taggtattgt aagaaatgtg gaaaaaagac
      181 tgaatatatt tgttcaggtt tatttagggt taatgctcaa cataaatatt tggatatatg
      241 gttaatatac aagtgttcaa attgtgattc aacatggaac tcaactatat attctcgtgt
      301 caatccaaaa agcttaaatc cagaaacact agaacaattc catacaaacg attataatct
      361 tgctaagcaa tatgctatga atacggaact tttacataga aatggagcag aagttggtat
      421 tccagagtat aaaatagtag gacaagaaat taatattaat gaccttacac aagttcatat
      481 tattagtaaa tatccatgtc agttaaaggt atcaacatta ttacgtgaga aactaggttt
      541 atctaggaag gtatttgacg aaatgttgga ttgtgggatt attagaagta actcaggagt
      601 tgatttaaag aaaggtaaat taaattatga aattatgtta gatataggca aggagaagca
      661 aaagtcttca aattgattag aataactaac ttaatatatt ttcaggaaca tcattt
//
LOCUS       DAEPLF010000420          700 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.425, whole genome shotgun sequence.
ACCESSION   DAEPLF010000420 DAEPLF010000000
VERSION     DAEPLF010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.425"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..423
                     /locus_tag="KR314_003734"
     CDS             <1..423
                     /locus_tag="KR314_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0330018.1"
                     /translation="TISKKYKMRSVNPLKAWVKEYNLHKEIKETPKGGLFMETRKTTL
                     DERVEIVKDCIEHDFNYNLIAKKYFVSYSQVRRWTLKYKENGIVGLEDRRGKAKKEDE
                     LTQLDEIKLELKIVKAEKHKLQLENAFLKKLKNIERGW"
     gene            417..>700
                     /locus_tag="KR314_003735"
     CDS             417..>700
                     /locus_tag="KR314_003735"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0330019.1"
                     /translation="MVITLSKNENKYKAIQSLFNEKKASLMQLCEIAKVNRSGYYKWL
                     NRDKSNLELENIKLATLITNIYEEKKGVFCSLRMTLQLNREYKLNVNHKRV"
BASE COUNT          307 a           78 c          126 g          189 t
ORIGIN      
        1 actatctcaa aaaaatataa aatgaggtct gttaatccac taaaagcttg ggttaaggag
       61 tataatcttc ataaggagat aaaagaaacc ccgaagggag gactctttat ggagactaga
      121 aaaacaacat tagatgaaag ggttgaaatt gttaaagatt gtattgagca tgattttaat
      181 tacaatttaa tagcaaaaaa atactttgtt tcatacagcc aagttagaag atggactctt
      241 aaatataaag aaaatggtat agttggttta gaagaccgaa gaggtaaagc aaaaaaagaa
      301 gatgagttaa ctcaattaga tgaaataaag ttggaattga aaattgtaaa agcagaaaaa
      361 cacaaattac aactggagaa tgcattctta aaaaagttaa aaaacataga aagagggtgg
      421 taattacact atccaagaat gaaaataaat ataaagctat tcaatcctta ttcaatgaaa
      481 aaaaagcttc gcttatgcaa ttatgtgaaa tcgctaaagt aaatcgttct ggatactata
      541 aatggctcaa tcgagataaa agtaatttag aacttgaaaa cattaaatta gcaactttga
      601 taactaatat atatgaagaa aagaagggag ttttttgctc tttaaggatg accttgcagt
      661 taaacagaga gtataaactt aatgtcaatc acaagagagt
//
LOCUS       DAEPLF010000421          700 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.426, whole genome shotgun sequence.
ACCESSION   DAEPLF010000421 DAEPLF010000000
VERSION     DAEPLF010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.426"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..589)
                     /locus_tag="KR314_003736"
     CDS             complement(<1..589)
                     /locus_tag="KR314_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBH0330020.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKK"
BASE COUNT          252 a          124 c           73 g          251 t
ORIGIN      
        1 gttttttatg atggaaatca taattatgat tttcaagatt tttatttact ctatttccaa
       61 tcttccatga caaaaacacc aataatataa atgatgctat ctttaaagga cttgttataa
      121 aaccttctat atcacttgcc acgtaactta taactataaa cattacaaat tttcctgcca
      181 acattgctgg cacaaaatct tttaaatctt ttttaccaaa agctgatgcg atagtaacta
      241 aacaaccagg cataaatgga caggcatacg ctataaataa taacttaaat ccctgtttat
      301 ccatccaact tattgctttt tcagttttac tatttctcaa cttattaaaa agcttactat
      361 cattaaatct gcttattaat aagaataatg aagttgtccc aagtcctgag ccaatccatg
      421 ataacaaaca tccaataaat aatccttgta ttgcagcatt tgctgtaact atagcaacaa
      481 gtggtaaggc tggtataaag ctttctataa atgcacttaa taagccgatt attgtggcta
      541 acacccaata attttgagtc aaatcaagta attcttgtat atactgcata ttgcttttcc
      601 ccttttttaa ctttaactat tattatatac tctatatgtt tattatatac caatctcaaa
      661 aaatgaatct aatattaaaa agtaaataat ctatataagt
//
LOCUS       DAEPLF010000422          672 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.427, whole genome shotgun sequence.
ACCESSION   DAEPLF010000422 DAEPLF010000000
VERSION     DAEPLF010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.427"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..>672)
                     /gene="yunB"
                     /locus_tag="KR314_003737"
     CDS             complement(<1..>672)
                     /gene="yunB"
                     /locus_tag="KR314_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBH0330021.1"
                     /translation="KNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTITVLAETKAL
                     ELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIASAIALEIQD
                     ELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFESSGINQTRH
                     RIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKNLGNVLPNTG
                     KNTNK"
BASE COUNT          195 a          104 c           88 g          285 t
ORIGIN      
        1 acttgttggt attctttcca gtgttaggta aaacatttcc tagatttttt tctggaatat
       61 ttacataact ttctggaaca tcacctacta ttatagtttc acaaataggt atttgagctt
      121 taatttgttt tgttgaagtt ataagtggta ttactacttt aacttgtgtt tgagcttcta
      181 aatatattct atgtctagtt tgatttatac ctgaagactc aaattccgtt ttaaagttta
      241 ctgatactgt tcctataggt tcaatagaca cttcaagttt aggaccgtat ttagctaata
      301 taggacttcc taatgctgtt cctataggta tgtaagaact tgctgtctta tcctttttta
      361 attcatcttg tatttctaaa gctattgcag atgcaatttc attcatcatt atagtgtttg
      421 cttgtatcat agttatttta ccattattat caagttgtat atccattaaa tcttcataat
      481 ttatttttcc ttctaccatc tcagcaacag ctttatttat cgacctattt gctagttcaa
      541 gagctttagt ctctgctaaa actgttattg tcggtcttaa agttttatct atatatataa
      601 aactgcctat aaatacagac aagaaaagaa ttactaaaaa aatagctatg atttttctaa
      661 attcattttt tg
//
LOCUS       DAEPLF010000423          661 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.428, whole genome shotgun sequence.
ACCESSION   DAEPLF010000423 DAEPLF010000000
VERSION     DAEPLF010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.428"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            46..396
                     /locus_tag="KR314_003738"
     CDS             46..396
                     /locus_tag="KR314_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBH0330022.1"
                     /translation="MIQKKIFMGFCGFLGFLSLRYFSSGNVTDLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNVELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            398..598
                     /locus_tag="KR314_003739"
     CDS             398..598
                     /locus_tag="KR314_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0330023.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
BASE COUNT          251 a           71 c           98 g          241 t
ORIGIN      
        1 aagatacaaa tatatctctt ttaaaattac taaggaggat aaaaaatgat tcaaaaaaaa
       61 atttttatgg gtttttgtgg tttccttgga tttttaagtc ttagatattt ttcatctggc
      121 aatgttactg atcttacata tattggattt tttgcttttt tctcaaactt tattatagca
      181 aaaataaatg gtgataaagc agatgaaagg tatgtacaag atgaaaaagc agctatggca
      241 tttacaggac agcttgctat tatagaatta ttcattttat ggtgtattac tatagtgtct
      301 aggaatgtag aattaatgtg tgttttacta tcaattacat atgcaatcac tttaaatgtt
      361 tatgctataa aactttatat tttagaggaa aaataatatg cccaaattca tttgtaacct
      421 aaaaaaatat agacaattaa aagaattaac tcaagaacaa ttagcagaaa aagtaggagt
      481 tagaagagaa accattatgc gtttagaagc aggaaagtat aatccttctt tgaaattagc
      541 aatagatata tcaaaaatag tagatacacc tattgatgaa ttgtttattt ttgattagtt
      601 tttgcattat taaaaatgtg ttttaaatac aaacttaaat tcaatcttta agtcactcct
      661 g
//
LOCUS       DAEPLF010000424          659 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.429, whole genome shotgun sequence.
ACCESSION   DAEPLF010000424 DAEPLF010000000
VERSION     DAEPLF010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.429"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
BASE COUNT          273 a           70 c          113 g          203 t
ORIGIN      
        1 ttatgagaat tatattataa aatattcata aatattttga gtttaaatta aataattatt
       61 ttatgcttag attaattgat atttaattaa tttaataaca aaaacagtga aagagtaaat
      121 aatattattt acagggggta aagcaaagta tgttaaataa attgtacttt tggccccgta
      181 catagagaaa tctatgtagg acacaacttt aaaaccagta aatcctaaag ccttgcaact
      241 acaataaaag atagaaagaa acttttatga atgtggtgaa aaccagaaac aatagtgagg
      301 atggtatatg gtgagagcct aagtacttaa aaatggacat ttggtcgcca agcctagaaa
      361 taggaaggtc aaacgactat ctcgagagag agtacactat aagcgattga tagtggaaac
      421 gggttgctct tagcaggtaa tgctgaagat gaagaaatag tctacgctca tatgaaagta
      481 tgaggtcact agatttgaag tgacatctat aaagtagcgt ttataggtaa atgtttaaaa
      541 cttgaaacat gtaaatactt atgaaaactt ataacttata atgattaaga ttgaaagatt
      601 taaattgaat taaatttact ataaaaaatt aaaaattcat agctataata ctcttatat
//
LOCUS       DAEPLF010000425          647 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.430, whole genome shotgun sequence.
ACCESSION   DAEPLF010000425 DAEPLF010000000
VERSION     DAEPLF010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.430"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            335..>647
                     /locus_tag="KR314_003740"
     CDS             335..>647
                     /locus_tag="KR314_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0330024.1"
                     /translation="MIVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALRDLDNAYKKFFKEKVGFPKF
                     KS"
BASE COUNT          277 a           65 c          113 g          192 t
ORIGIN      
        1 tatcggtagt ggaagtgggt agaccttagc ggataatgcc gaaggataag atatagtctg
       61 tgcttgcatg aaagtgtaag aagttcataa gagaactgca taggtactag cgaacttatg
      121 tgaacgacaa cctcaaaaat gataaaagtt aagacttacg gttcttatat atgttattta
      181 aaaataaata taaaaaacct tcaaaatact tgttttaagt aattgaatat tttaatatta
      241 tgataggtga tgaaaaatgg aagttactca tggtagagga tatgcatata gtatgtcaaa
      301 tatagaaata atcagaaaaa acaagtaaag tgaggtgata gtagtggaaa aagcttataa
      361 gtttagaatg tatccaaata aaaaacagca agagttaatt aacaaaactt ttggatgttg
      421 tagatttgta tataataagt atcttgctaa aagaatagaa gtttataaaa acaataaaga
      481 aacttttact tataaacagt gtagttctga tttgaccaac ttaaaaaaag aattaaagtg
      541 gcttaaagaa cctgacaaat tttcactaca aaacgcctta agagatttag ataatgcata
      601 taaaaaattc tttaaagaaa aagtaggatt tcctaaattt aaatcaa
//
LOCUS       DAEPLF010000426          632 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.431, whole genome shotgun sequence.
ACCESSION   DAEPLF010000426 DAEPLF010000000
VERSION     DAEPLF010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.431"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            148..>632
                     /locus_tag="KR314_003741"
     CDS             148..>632
                     /locus_tag="KR314_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0330025.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNTINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     M"
BASE COUNT          265 a           49 c          111 g          207 t
ORIGIN      
        1 tacaagagat agatatattg gttttaattt tatagaaata tacaaagata ttttaagcta
       61 atatatagta atttatatag cattataaac tagaatcata taaaataagt taactacaat
      121 cataaattaa aaaggagaaa tgttaagttg atagaaggaa taaaatttat aaaggcagaa
      181 gaaaagtata tttattcgta ttggcaaact tttgataaaa tagcaaaaga gaggaaatat
      241 ctagctatgg atgaagcctt tccttttgaa gaaacagttg aatttataaa aaatactata
      301 aataaaaact taccacaact gtttataatt gatttagaat cagataactg tattggttgg
      361 tgtgacgttt tacctaaaac tgaaaaagtt ggttatttag gcatgggtat tcttaaagag
      421 tatagggaaa aaggtatcgg aagtagtctt ttaaaacaaa ttattgattt atcaaaagaa
      481 tatgggtatg aaaaaattga gcttgatgta tttaaaagta atagtagagc tattcatgtt
      541 tataaatctt tagggtttgt agaggtaaat acaatatcta gtggttttac atggaatgat
      601 agacctgtaa aagaagaagt aattcaaatg ga
//
LOCUS       DAEPLF010000427          543 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.433, whole genome shotgun sequence.
ACCESSION   DAEPLF010000427 DAEPLF010000000
VERSION     DAEPLF010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.433"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            41..>543
                     /locus_tag="KR314_003742"
     CDS             41..>543
                     /locus_tag="KR314_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBH0330026.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTS"
BASE COUNT          182 a           73 c           78 g          210 t
ORIGIN      
        1 taatacttta atacatttaa tacaaaagga gtgatttatt gtggaaagaa ctcaacaatt
       61 aaaagaaagc tttattgctt caattcctat attcttaggt tatataccta taggtatagt
      121 aggaggaata ctattacaaa aatctggtct ttcacctttt caaatagcac taatggtaac
      181 tttagtattt ggaggaagtt ctcaattcat tgcagcttca atgatttcta ctggtgcaag
      241 tgtaacatct attgttttga ctacatttat agttaactta agacattttc taatgagttc
      301 taatttgaac atgtacataa aaaataaaag tccaaaattt atattgccat tttgtcatac
      361 tataacagat gaaacttttg cagttaatta tgaaaaattt actgctggaa attggtctga
      421 tagaaatgct atttatctta atttcttctg cttaatttca tcagttttag ctaattttat
      481 tggagcattt ttaggagaaa gtatatctat agacagttct atttctggct tcatattgac
      541 ttc
//
LOCUS       DAEPLF010000428          525 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.435, whole genome shotgun sequence.
ACCESSION   DAEPLF010000428 DAEPLF010000000
VERSION     DAEPLF010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.435"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(260..376)
                     /gene="rrf"
                     /locus_tag="KR314_003743"
     rRNA            complement(260..376)
                     /gene="rrf"
                     /locus_tag="KR314_003743"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(385..458)
                     /locus_tag="KR314_003744"
     tRNA            complement(385..458)
                     /locus_tag="KR314_003744"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Gly,seq:tcc)
BASE COUNT          206 a           89 c           62 g          168 t
ORIGIN      
        1 ttaacataaa tttctacttt gttttttaac cttttaattt ttgaaattat atcaaagtgt
       61 ttttacccaa aattataaat aattacataa aaaataaact tctaaaaaaa ttataaaaac
      121 aaaaaataaa aaaattaaat atattgaata tatttaatta tcttcactat atcatttatt
      181 gccaaaaaaa tctataaaag acaaataaaa acatataaca attgtaggtt tattaaattt
      241 aatacaacaa aaaaagatta cgtggctacg tcctactctc ccaggcagtt tcccaccaag
      301 taccatcagc actagagagc ttaacttctg tgttcggaat gggaacaggt gtatcctctc
      361 tgctattgta accacataat cttttggagc gggtgaaggg gatcgaaccc tcacagctgg
      421 cttggaaggc cagaactcta ccattgagct acacccgcat taaattttta taaattagta
      481 aaactaattt tttagagtta gcaccctaaa aactacatat atatc
//
LOCUS       DAEPLF010000429          514 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.436, whole genome shotgun sequence.
ACCESSION   DAEPLF010000429 DAEPLF010000000
VERSION     DAEPLF010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.436"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            159..266
                     /locus_tag="KR314_003745"
     CDS             159..266
                     /locus_tag="KR314_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0330027.1"
                     /translation="MGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            268..>514
                     /locus_tag="KR314_003746"
     CDS             268..>514
                     /locus_tag="KR314_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0330028.1"
                     /translation="MVVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNNKETFTYKQCSSDLNNLKKELKWLKEPDKFSLQNAL"
BASE COUNT          216 a           51 c           86 g          161 t
ORIGIN      
        1 ggttcttata tatgttattt aaaattaaat ataaaaaacc ttcaaaatac ttgttttaag
       61 taattgcata ttttaatatt atgataggtg atgaaaaatg gaagttactc atggtagagg
      121 atatgcatat agtatgtcaa atatagacag ccagcattat ggggagacat ctttggaatc
      181 ctagttattt cgttgctact gtatcagaaa atacagagga gcagataaga aaatatatta
      241 ataatcagaa aaaacaagta aagtgaggtg gtagtagtgg aaaaagctta taagtttaga
      301 atgtatccaa ataaaaagca acaagagtta attaacaaaa cttttggatg ttgtagattt
      361 gtatataata agtatcttgc taaaagaata gatgtttata aaaacaataa agaaactttt
      421 acttataaac agtgtagttc tgatttgaac aatttaaaaa aagaattaaa atggcttaag
      481 gaacctgaca aattttcact acaaaacgcc ttaa
//
LOCUS       DAEPLF010000430          454 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.438, whole genome shotgun sequence.
ACCESSION   DAEPLF010000430 DAEPLF010000000
VERSION     DAEPLF010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.438"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..372)
                     /locus_tag="KR314_003747"
     rRNA            complement(<1..372)
                     /locus_tag="KR314_003747"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          120 a          109 c           73 g          152 t
ORIGIN      
        1 aggactatta ccttctgtgg tgggtctttc caaacctctt caattacgat acctcttcgt
       61 tatgatatgt ccgcaacccc aataaagaaa actttattgg tttgggctaa tccgcgttcg
      121 ctcgccgcta cttacggaat cgaatttctt tctcttcctt caggtactta gatgtttcag
      181 ttccctgagt tcccctcatt aagctatgta ttcacttaat gatacttaga cattactcta
      241 agtgagtttc ctcattcgga aatcttcgga tcaaagttta cgtgcaactc cccgaagctt
      301 atcgcagctt atcacgtcct tcatcggctc ctagtgccaa ggcatccgcc ctgcaccctt
      361 aataacttga ccagttaaaa aggtttgata gattagaagc tatcaacttc gataatttta
      421 aaaagttatc agctttatat attacaaatt agat
//
LOCUS       DAEPLF010000431          420 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.439, whole genome shotgun sequence.
ACCESSION   DAEPLF010000431 DAEPLF010000000
VERSION     DAEPLF010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.439"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..>420
                     /locus_tag="KR314_003748"
     rRNA            <1..>420
                     /locus_tag="KR314_003748"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          106 a           97 c          130 g           87 t
ORIGIN      
        1 cgcctcccaa aaggtaacgg aggcgctcaa aggttctctc agtacggtcg gaaatcgtac
       61 gtagagtgta aaggcaaaag agagcttgat tgcaagacat acaggtcgag caaggatgaa
      121 aatcggactt agtgatccgg tggttctgcg tggaagggcc atcgctcaac ggataaaagc
      181 taccctgggg ataacaggct tatctccccc aagagtccac atcgacgggg aggtttggca
      241 cctcgatgtc ggctcatcac atcctggggc tgtagtaggt cccaagggtt gggctgttcg
      301 cccattaaag tggtacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc
      361 gtcgcaggcg taggaaattt gagaagacct gtccttagta cgagaggacc gggatggacg
//
LOCUS       DAEPLF010000432          408 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.440, whole genome shotgun sequence.
ACCESSION   DAEPLF010000432 DAEPLF010000000
VERSION     DAEPLF010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.440"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            116..307
                     /locus_tag="KR314_003749"
     CDS             116..307
                     /locus_tag="KR314_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBH0330029.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
BASE COUNT          145 a           34 c           68 g          161 t
ORIGIN      
        1 ttttttattt tttgtcaaaa aaaacaaaaa atacgtattt attttttcat ttcgggaaaa
       61 ctaaacacag gaattatgga ctttataaaa atttaattct tgaggagtga tataagtgaa
      121 aaaagttgct ttaattttag ctataatagg tgcgcttaac tggggtgcaa ttggtattct
      181 aggtacagac ttaataggta gtatctttgg tggaacatat gaaatggtaa gtagaataat
      241 atacttcatt gtaggtttag ctggattata cttaatacca agtttaatgt ctgatgatag
      301 agaataattt atgcttttat gttaaatacc tcattctaaa tgtttagaat gaggtattta
      361 attttataaa aatattaatt tgtttctttt ttaatggtaa aatagata
//
LOCUS       DAEPLF010000433          364 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.441, whole genome shotgun sequence.
ACCESSION   DAEPLF010000433 DAEPLF010000000
VERSION     DAEPLF010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.441"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="KR314_003750"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="KR314_003750"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..362
                     /locus_tag="KR314_003751"
     tRNA            291..362
                     /locus_tag="KR314_003751"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
BASE COUNT          117 a           61 c           78 g          108 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agcc
//
LOCUS       DAEPLF010000434          343 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.443, whole genome shotgun sequence.
ACCESSION   DAEPLF010000434 DAEPLF010000000
VERSION     DAEPLF010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.443"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..>343
                     /locus_tag="KR314_003752"
     rRNA            <1..>343
                     /locus_tag="KR314_003752"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           81 a           76 c           98 g           88 t
ORIGIN      
        1 tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa
       61 cccttgtctt tagttgccat cattaagttg ggcactctag agagactgcc agggataacc
      121 tggaggaagg tggggatgac gtcaaatcat catgcccctt atgcttaggg ctacacacgt
      181 gctacaatgg gtagtacaga gggttgccaa gccgtaaggt ggagctaatc ccttaaagct
      241 actctcagtt cggattgtag gctgaaactc gcctacatga agctggagtt actagtaatc
      301 gcagatcaga atgctgcggt gaatgcgttc ccgggtcttg tac
//
LOCUS       DAEPLF010000435          261 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.denovo.444, whole genome shotgun sequence.
ACCESSION   DAEPLF010000435 DAEPLF010000000
VERSION     DAEPLF010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.denovo.444"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            complement(<1..>261)
                     /locus_tag="KR314_003753"
     rRNA            complement(<1..>261)
                     /locus_tag="KR314_003753"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           48 a           79 c           55 g           79 t
ORIGIN      
        1 ctcgctttcg ctacggctcc gcaccttaag tgcttaacct tgctacttaa cgtaactcgt
       61 tggcccgttc tacaaaaagt acgcggtcac acaagaaatg tgcttccaca gcttgtaagt
      121 gcagggtttc aggttctatt tcactcccct cccggggttc ttttcacctt tccctcacgg
      181 tactatacgc tatcggtcac taggtagtat ttagccttgg agggtggtcc ctcctgcttc
      241 ccacagggtt tcacgtgtcc c
//
LOCUS       DAEPLF010000436         6397 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.guided.192, whole genome shotgun sequence.
ACCESSION   DAEPLF010000436 DAEPLF010000000
VERSION     DAEPLF010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.guided.192"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            19..720
                     /gene="vanR"
                     /locus_tag="KR314_003754"
     CDS             19..720
                     /gene="vanR"
                     /locus_tag="KR314_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBH0330030.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKAQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            770..1852
                     /locus_tag="KR314_003755"
     CDS             770..1852
                     /locus_tag="KR314_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0330031.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            2113..3213
                     /gene="vanG"
                     /locus_tag="KR314_003756"
     CDS             2113..3213
                     /gene="vanG"
                     /locus_tag="KR314_003756"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBH0330032.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            3210..4016
                     /locus_tag="KR314_003757"
     CDS             3210..4016
                     /locus_tag="KR314_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0330033.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            4034..6172
                     /gene="vanT"
                     /locus_tag="KR314_003758"
     CDS             4034..6172
                     /gene="vanT"
                     /locus_tag="KR314_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBH0330034.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
BASE COUNT         2350 a          723 c         1111 g         2213 t
ORIGIN      
        1 gagagtgaaa aataatctat gaacgaaaat attttgattg tggatgatga aaaagaaata
       61 gcagatttaa ttgaagtata tttaaaaaat gaaggtttta cagtatacaa gttttataca
      121 ggtaaggaag ctctagaatg tattgaatct aaagatttgg atatggctat tttagatatt
      181 atgcttcctg atattgatgg atttcatatt tgccagaaaa ttcgagaaaa atactattat
      241 cctatcatta tgttaacagc taaaattgaa gatatagaca agataatggg tttaaccata
      301 ggtgctgatg attatataac aaagccattt aaccctttgg aagttgttgc aagagtaaag
      361 gcacaattaa gaagatatat gcgatataat aattcttatg aacaacagtc gataattgtt
      421 aatgagtatg atattaaagg attgattatc aataaagaaa cacataaatg tagtttattt
      481 gggaaagaag tagcattaac accgattgaa ttttctattc tttggtattt atgtgaacat
      541 caaggaaaag tggttccttc tgaagaattg tttgaagctg tatggggaga aaaatatctt
      601 gataataata acacagttat ggctcatata gggcgattac gtgaaaaaat tcatgaacct
      661 tctaaaaacc cgaaatttat aaaaactgta tggggagtag gttatacaat tgaaaagtaa
      721 aaatattaaa gtcaatcctg actttactca agtgagaaat aaaattttta tgagaacagc
      781 tttaatggca tttatagcca ttattttcat atggatttta tattctttta tattgtatgg
      841 taagttttct atctggatag tatcattctt tgaaaatgct tttcgtatgg aatataaagc
      901 tgcattgaat ttatatcagc agattttccg taatagcatg gagctcttca tgatactagc
      961 tatggtgatt gtgtttttta taatattccg tgtttattta aattggttta caaaatattt
     1021 tagtgaaata aatcaaggta ttgactctct tatcaaagaa gatgttggag aagtggcttt
     1081 atctccagag ttattggcaa tagaaaagaa aataaactca attaaacata tacttgaaca
     1141 acgtaaattt gagacacaaa tggcagaaca acgtaagaat gaacttattg tttatcttgc
     1201 acatgattta aaaacaccac ttacatcagt tatagggtat ctgacactac ttcgagatga
     1261 aagtcagatt tctgaagaac tgcgaaaaaa atatttatct atttcgttgg ataaggcaga
     1321 gcgtcttgaa gatttaatta atgaattctt tgaaattaca aggtttaatt tatcaaatat
     1381 atcacttgaa tacagtacag taagcttaac tcgtatgctt gaacaactaa catatgagtt
     1441 taaacctact ttgatgagta agaatttaaa atatacactg aatgttacac caaatatgat
     1501 gataaggtgt gatgttaata aaatgcaacg tgtatttgat aacttattac gaaatgctgt
     1561 ttattatagt tttgaagata ctactattga aataacagct gtacaggagg atgattatat
     1621 aaagcttaaa tttataaatc atagcaatac gattccagag gaaaaacttg agcgtatttt
     1681 tgaacaattt tacaggttgg atacatctcg taattcaaac agtggtagag ctgggttggg
     1741 acttgctata gctaaagaaa tagtggagct tcataacggg acaataacag ctcatagcga
     1801 gaacaatata attaccttta aggttattat tccgattgta ggaaatttgt aagaatttaa
     1861 gtataaaaaa ataggaaatt ctttaggata aataaataaa gagcactctt gttattgata
     1921 taattactgt atcaaaacaa gagtgctttt attttgatac agtaattata tcaatagaaa
     1981 aatgtgaaaa tatattatgt ggaacagatg caattgtaag tgaaggttga ttggatttga
     2041 aatagaaaaa gtattttaac atgtttctgt agattgtata aaaataaata aatcaacaaa
     2101 ggagaatcaa agatgaataa taaaaaaatt gcagttatat ttggtggaaa ctcaacagaa
     2161 tatgatgtat cattacaatc agcttttgca gtacttgaga atttgaatac ggaaaaatat
     2221 gatattatac caattggaat tacaagggag ggtaattggt atcattatat tggagattat
     2281 aataatatcc agaataacac ttggtttgaa agggatactg atataattcc agttattgtt
     2341 tcgcagaacc gtgtcaaaaa tggtattatt gaatttttaa ttcctgatgc tgtttctcag
     2401 aatcatattc caaatagtgc tattgaatca tgtgaaaatg atattaaata cactaaactt
     2461 gattttgtat ttcctgtatt gcatggaaaa aatggagaag atggaacagt gcaggggtta
     2521 tttgaattgg caggtataca ggttattgga tgtggtactc tttcttcagc attatgtatg
     2581 gacaaagaca aatcacataa acttgttcat tcagaaggta ttaaagtacc aaaatcaata
     2641 ttactaaaat actcagataa atttaatgtg gctgaactgt caaaagactt aacttatcca
     2701 ttatttataa aacctgtgcg tgcaggttca tcatttggta ttagtaaaat ttataaaaaa
     2761 gaagagttac aagtagcagt agacttggct tttgaacatg ataatgaagt aataattgag
     2821 gaaaatattg atggttttga ggtagggtgt gcagttttag gaaacgatga cttacttgtt
     2881 ggaagagtag atgaaataga attgtcaaaa ggattttttg attatacaga aaaatataca
     2941 ttgaaaagtt caaaaattca tatgccagct agaattgatt tggattgtga gaaaagaata
     3001 caagagacag caaaaattat ttatagaact cttggatgtt ctggatttgc tagagtagat
     3061 atgtttttaa ctccatctgg tgaaattatt tttaatgaag tcaatacaat tcctggcttt
     3121 acttctcata gtcgttatcc gaatatgatg aaaggtattg gactgtcttt tgaagaaata
     3181 ttggataaga taattgaggt gtacataaaa tgatgaataa tattttatta gataaaaata
     3241 aagaagataa aatgacaaat actatcttat tgactaaaga taaagtttat gatgggaatc
     3301 taattctagt aaatgctttt ttaccagtta aaacatctga agatatagat ttaattcctg
     3361 tagatacaag gtttccaagt attcttatga aacgtgaagc tacaaatatt ttacaaaata
     3421 ttttaaaaag tatatgtgga ataaatgaaa tagtacctgt aagtggatat cgtactgcag
     3481 aggaacaaca agatatatat tcaagctctc tgcgtgacaa tggaaaagac tttactaaaa
     3541 agtttgtagc acttccaaat cacagtgaac atcagacagg gcttgctatt gatttagggt
     3601 tgaagaaaga ggttatagat tttatctgcc ctgaatttcc ttataatggt atttgtaatg
     3661 agttccgtaa ggcagcacta cattatggtt ttatagagcg ttatcagtgt aaaaaagaag
     3721 aaataacagg aatatctcaa gaaccatggc attttcgtta tgtcggatat ccacattctg
     3781 agattatcaa taaaatgaat ctcgcccttg aagaatatat agaatatatt aagagatttt
     3841 cgtatacaaa ttgtttaaaa atagaagata aagaaaaaat tatagaaatc ttttatgtgc
     3901 ctattcatac taaagaagaa gtgagaatct ctatacctga acaatgtgta tatcaaatat
     3961 cgggtaacaa taaggatggt tttattgtga cattttggag aaatagaaag gactaagaaa
     4021 tggaggaaca agaatgatta ggaataaatc ttatgcaggg cttgattatt ttaggattat
     4081 agctgcatta cttataattg caattcatac atctccatta ttatcatata gtaaaatggc
     4141 tgactttgtg ttaactagta taattgctcg tattgctgtg ccattctttt ttatgctatc
     4201 aggctttttt ctaatttcaa catataatta taattgtaat aaactgaaat cttttctaaa
     4261 aaatgtatta tttatttacg ggatagctat tgtaatttat attcccttaa atatttacac
     4321 tggatatttt tccatgaata atttagtgcc caatattatt aaggacattg tatttgatgg
     4381 gactttatat catttatggt atctacctgc atcaattatt ggtgcaagta ttgcatactt
     4441 tcttgtaaaa aaactaggtt taaagtactc attagtaatc actattgtta tttatgtaat
     4501 tggtatgttt ggtgatagtt attatggttt tttagaaaaa ataccactat taaagaattt
     4561 atatgggtat atttttgaag tatcagatta tacacgaaat ggtatatttt ttgcacctgt
     4621 atttttcata cttggtggaa taatatctaa taaatctaat tatatgtcaa ctaaaaatag
     4681 cttgattgga tttcttatat cattcttact tatgttatgt gagggaatgt ttttacataa
     4741 actaggggta cagcgtcatg atagcatgta tattatgtta ttgccatgta tgtatttctt
     4801 atttacagcc ttaacatttt taaaaggttc tcgagctagg ttattgcgaa catcatcatt
     4861 aattatttat attatacatc ctatgatgat tgttgttatt cgtatgtttt ctaaaatatt
     4921 cggaatacaa actgtcttgg ttgacaatag ccttattcat tatatagtgg taagtatagc
     4981 atcttttata gcttctataa ttattatatt gattcttaaa cctctaaaaa aagacttgaa
     5041 aggagaacat aaggtaaata ccaatagagc ttggattgaa attgatttaa ataatttgga
     5101 acataatgta aagatgatac aaaaagctat gccagaagat tgtgaattga tggcagttgt
     5161 aaaagctaat gcctatggtc atagtgcttt tgaagtagcg acttgtgcaa accaaatagg
     5221 tgtaaaagca tttgcagtag caacaataga tgaggggatt gatttacgtc gatatggaat
     5281 actaggagaa atattgattc ttggatatac taacccaata agggcaaagg aacttcataa
     5341 atacaatctt acacagagtg ttattgacct taatcatgct atctctttaa acaaacagaa
     5401 atacaacata aaatcacaca ttaaaattga tacaggtatg cacagactgg gttttgatgt
     5461 taatgatgtg aggtcaattt taaaggcatt taaattaaaa tacttagata tatgtggcat
     5521 atttacacac ttgtgtgttt cagatagtct gaaagatgaa gatgtagact ttacaaataa
     5581 acaaattgaa tgtttttata agttaattga ttttctgtta aaaaaaggta taaaaattcc
     5641 taaagtacat attcaaagta gttatggttt ttttaattat cccaatctta agtgtaatta
     5701 tgtaagaatt ggaattgcat tatacgggat attaagttct ccaaatgata cgactaaatt
     5761 gcaacttgat ttacgacctg ttttatcctt aaaatcacaa gtaattctta ttagaaaaat
     5821 acaaaaagat gatagttttg gatatggaag agtctttatt gcaagcagag atagcgtaat
     5881 tgctatatta tctattggct atgccgatgg tatacctaga aatttatcct gtggaaaaag
     5941 ttacgtaata ataaatggtt gtagagcagc aatagtgggt cgaatttgca tggaccaact
     6001 tgctgttgat attacagata tacctaatgt agaagttggt aatacggcaa taataatagg
     6061 aagagataat ttatctgaat tatctgcttc tgaagttgca aataactcat gtagtatatc
     6121 gaatgaatta ttgagcagag taggaagaag gttaaatgtt ataaagaagt aactcaatta
     6181 aatagtaata tttgttgtac tgtgtaattt attttactct gactagacat ttttagtaat
     6241 attatgtcta atatatattt tttataactt acatgacaat ttaagttata aaaaggtgat
     6301 gtttcagatt tttattttga aacatcacct tttttgttat aatatataat aaaataatta
     6361 taccttaact gtaaacttat atagattatt tactttt
//
LOCUS       DAEPLF010000437         2522 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.guided.328, whole genome shotgun sequence.
ACCESSION   DAEPLF010000437 DAEPLF010000000
VERSION     DAEPLF010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.guided.328"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            83..298
                     /locus_tag="KR314_003759"
     CDS             83..298
                     /locus_tag="KR314_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBH0330035.1"
                     /translation="MVVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDKALEEYIRKYLPDKL"
     gene            315..587
                     /locus_tag="KR314_003760"
     CDS             315..587
                     /locus_tag="KR314_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBH0330036.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            589..738
                     /locus_tag="KR314_003761"
                     /pseudo
     CDS             589..738
                     /locus_tag="KR314_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            799..1140
                     /locus_tag="KR314_003762"
                     /pseudo
     CDS             799..1140
                     /locus_tag="KR314_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            1265..2002
                     /gene="erm(B)"
                     /locus_tag="KR314_003763"
     CDS             1265..2002
                     /gene="erm(B)"
                     /locus_tag="KR314_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBH0330037.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            2148..2432
                     /locus_tag="KR314_003764"
     CDS             2148..2432
                     /locus_tag="KR314_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HBH0330038.1"
                     /translation="MPTKRSGPLVGKCPKCGNNIVLKKSFYGCSNYPECTFTLAEHFR
                     KKKLTKTNVKELLEGKETLVKGIKTKDRKSYNAVVKIGEKGYIDFISFSK"
BASE COUNT          956 a          379 c          482 g          705 t
ORIGIN      
        1 tcacaagtga ttaatcacaa atcacttgtg atttgtgatt gttgatgata aaataagagt
       61 aagaagaaat agaaagaagt gagtggttgt gggaaattta ggcgcacaaa aagcaaaacg
      121 aaatgataca ccaatcagtg caaaaaaaga tattatgggg gataagacgg ttcgtgttcg
      181 tgctgacttg caccatatta taaaaatcga aacagcaaag aatggcggaa acgtaaaaga
      241 agttatggat aaagccttag aagaatatat tcggaaatat ttacctgaca aactttaaaa
      301 ggagtgaaaa tgaaatggca gttacttatg aaaaaacatt tgaaatagag atcattaacg
      361 aattatcggc aagcgtttat aatcgagtat taaactatgt tttgaatcat gaattaaata
      421 aaaatgactc tcaattattg gaagtcaatt tattaaacca attaaagctt gcaaaacgtg
      481 taaatctttt tgattattct ttagaagaat tacaagccgt tcatgagtat tggcggtcaa
      541 tgaatcgcta ttcaaaacaa gttttgaata aagagaaagt ggcttaatat ggcaaatata
      601 gtcaatttta ctgacaaaca atttgaaaat cgcttaaatg ataatttaga agaattggtt
      661 caaggaaaaa aagcggttga atcgccaacc gcttttttac ttggtgggca accagggtca
      721 gggaaaacga gtttgcgaag aagatgattg agcaggcagg aacaaagtga taaattcaaa
      781 ttgtgaggga aaatatagat gaatcaagga agaattattg tgattacagg ctcaccgggg
      841 acaggaaaaa caacaactgc gtcaattgtc gcaaaggaat cagatatgga taaatcagtg
      901 catatgcaca ccgatgactt ttttcattac ttaagcaaag gcgcaatacc accacatttg
      961 ccagaatcca acaagcaaaa tctggttgtc attgaagcct ttttagaggc tgcaaagcga
     1021 tatgctcgtg gcggatatga tgtaattgta gatgggattg tcgggccatg gtttttggag
     1081 ccatggaaag ctcttgccca agaagattac gaggtacact atattgtatt aagagcgagt
     1141 atggaaataa gacttagaag caaacttaag agtgtgttga tagtgcagta tcttaaaatt
     1201 ttgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa gaaggagtga
     1261 ttacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa aagtactcaa
     1321 ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa ttggaacagg
     1381 taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt ctattgaatt
     1441 agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaatactc gtgtcacttt
     1501 aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata aaattgttgg
     1561 gagtattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg aaagccatgc
     1621 gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg atattcaccg
     1681 aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta agctgccagc
     1741 ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta cccgccatac
     1801 cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa aatgggtcaa
     1861 tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga aacacgccaa
     1921 agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta atagttatct
     1981 attatttaac gggaggaaat aattctatga gtcgcttttg taaatttgga aagttacacg
     2041 ttactaaagg gaatgtagat aaattattag gtatactact gacagcttcc aaggagctaa
     2101 agaggtccct agcgcctacg gggaatttgt atcgataagg ggtacaaatt cccactaagc
     2161 gctcgggacc ccttgtagga aaatgtccta agtgtggcaa caatattgta ttaaaaaaat
     2221 cgttttatgg ttgttcaaat tatcctgaat gtaccttcac tttagctgaa cattttagaa
     2281 agaaaaaact caccaaaaca aatgtaaaag aattactaga gggaaaagaa accctggtaa
     2341 aaggaatcaa aacgaaagat agaaagtcct acaatgccgt tgtaaaaatc ggagaaaagg
     2401 gatatattga ttttatctct ttttcaaaat aagcataaaa gccctttaaa gagggctttt
     2461 atatattaat cacaaatcac ttatcacaaa tcacaagtga ttaatcacaa atcacttgtg
     2521 at
//
LOCUS       DAEPLF010000438         1243 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.guided.393, whole genome shotgun sequence.
ACCESSION   DAEPLF010000438 DAEPLF010000000
VERSION     DAEPLF010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.guided.393"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            35..973
                     /gene="blaCDD"
                     /locus_tag="KR314_003765"
     CDS             35..973
                     /gene="blaCDD"
                     /locus_tag="KR314_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBH0330039.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT          536 a          103 c          204 g          400 t
ORIGIN      
        1 actataaact tatgtagagg agatggtata aagtatgaaa agaaaaaata attttatttg
       61 gatagttatt cttctagttg ttgtagctat gggagtatat tatgtaggaa gaaagcataa
      121 caacacaaaa caaaaacatg ataagaatca tataaactct gaattaaata aatccaaagt
      181 tgaaaacaat gataaaaaca aaaaagaaaa aattaatatg gttgactata gtgactgttt
      241 tgaaggtatt agtggaggag ctatttttta taatactaaa aataaagaat ataatatata
      301 taacaaagaa ttaattgaaa caagaaggtc accatgttct acttttaaaa tagtttcaac
      361 tttaattggt ttagaaaaag gggtaataaa ctcaaaagaa tcagttatgg gatatgatgg
      421 tacagagtat ccaaataaaa attggaataa aaatcttagt ttagaagagg catttaagga
      481 gtcttgtgtt tggtactaca aaaaattgat agataaagtt gatgctaaat ctgtccaaaa
      541 cattttagat gatttaaagt atgggaattg cgatattagc gaatgggaag gagatttaaa
      601 aaatggaaag ggacatttaa atggattttg gttagaatct tcattacaaa tatctccaaa
      661 agaacaagtg caaacaatgg caaaaatatt tgagggagat acaaacttta aaaaagagca
      721 tattaatatt ttaagagata ttatgaaaat agatgtaaat gataaaaaca taaatgtata
      781 tggaaaaact ggaacaggat ttgatgaaaa aaataaacgt gtggatgcat ggtttgttgg
      841 tatgctagag cgtgaaggtg acacctatta ttttgctatt aaatcagatg attccaataa
      901 agaaattaca gggccaaagg taaaggaaat tgcaattaat ataattaaga aatattatag
      961 tgtaagggaa taaacttaat tttatttgta aatattattt acctatagtc tgtaatattt
     1021 attaaataat tttactaaat tgagtctata tagagcaaaa ctcctttagt acataaaata
     1081 cttgctattg attattaaat tgaataccta tagatttaaa gttaatacct tttaaagact
     1141 atgtaatata ttaaaagtat aaataaaata tattgcatag tttttgtttt atattataat
     1201 tttaaataat gtgcaaaaac taaacaatat ttttaatagt aat
//
LOCUS       DAEPLF010000439          998 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.guided.407, whole genome shotgun sequence.
ACCESSION   DAEPLF010000439 DAEPLF010000000
VERSION     DAEPLF010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.guided.407"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            89..787
                     /gene="vanR"
                     /locus_tag="KR314_003766"
     CDS             89..787
                     /gene="vanR"
                     /locus_tag="KR314_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBH0330040.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
     gene            823..>998
                     /locus_tag="KR314_003767"
     CDS             823..>998
                     /locus_tag="KR314_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0330041.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFS"
BASE COUNT          381 a          112 c          160 g          345 t
ORIGIN      
        1 ttaataaatt ctaatgtaaa aaatcatata aaaaaattaa atttagtata atatatataa
       61 atatatatag aaaggatgtt taaagtctat gagtggaaaa attttagttg tagatgacga
      121 acgtgatata gctgatttaa tagaagtcta ccttacaaat gaaaactata ttgtttataa
      181 attttattct ggtcttgatg cactaaactg cattaattta aatgaatttg accttgcaat
      241 tttagatgtt atgttgccag gagtaaatgg attttcttta tgccaaaaaa tccgagaaaa
      301 atatacttat ccaatcatta tgttaacagc taaggatgaa gaaacagaca agattacagg
      361 cttaacttta ggtgcagatg attatgttac taaacctttc cgacctttgg aacttgttgc
      421 tagagtgaag gcacagatta gaagatataa gaaatacagt ggaagtttac aatctggtga
      481 agaactttca catatttttt cttttttagg tctggaattg aatacccaaa catatgaatg
      541 ccatttaaat ggtaaagctc tttcacttac tccaacagag ttttctattc tacatgtatt
      601 attgaaaaat aaaggctctg tggtaaattc agaaaatctt tttcatgcta tctggaaaga
      661 tgaatattac aataaaagta acaatactat aacagttcat attcgccatc ttcgtgaaaa
      721 attaggtgat actgtagatg aagcaaaata tataaaaaca atttggggtg ttggttacaa
      781 gatttgacac ctatttttct tgtcaagaag ggatgtagta ttatgaaaaa gattaataat
      841 tttacttata ggaaactaag agataaatat aaatttataa aaagagaaaa aaatacagag
      901 gcgtatttaa agtataaatt atatataatg ttagctatct atacaggtat agtattggtt
      961 ttaatgttta tggctgatta tacgttttca aatttttc
//
LOCUS       DAEPLF010000440          627 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain 410A2
            SAMN05832964-rid12467873.guided.432, whole genome shotgun sequence.
ACCESSION   DAEPLF010000440 DAEPLF010000000
VERSION     DAEPLF010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05832964
            Sequence Read Archive: SRR4300563
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001068032.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001068032.1
            Genome Coverage       :: 296x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 22:45:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,768
            CDSs (total)                      :: 3,683
            Genes (coding)                    :: 3,628
            CDSs (with protein)               :: 3,628
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 22 of 55
            Pseudo Genes (incomplete)         :: 29 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05832964-rid12467873.guided.432"
                     /strain="410A2"
                     /isolation_source="stool"
                     /db_xref="taxon:1496"
                     /country="USA: California"
                     /collection_date="2013-06-25"
     gene            <1..587
                     /locus_tag="KR314_003768"
     CDS             <1..587
                     /locus_tag="KR314_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBH0330042.1"
                     /translation="QTVYLKNVITRENIEVGDYTIYNDFYNDPRDFEKNNVLYHYPIN
                     NDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEWKLDINHITDAWDNKGN
                     IIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSIVGGVPAKIIKKRFNND
                     TISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT          254 a           71 c           96 g          206 t
ORIGIN      
        1 atcaaactgt atatcttaaa aatgtaataa caagggaaaa tatagaagtt ggagattaca
       61 caatatataa tgacttttat aatgacccaa gagattttga gaaaaacaat gtattatatc
      121 attaccctat aaacaatgat aaattaataa ttggtaagtt ttgctcaata gcatgtaatg
      181 caaaatttct aatgactagt ggaaatcact caatgaaatc aaaatccaca tatacttttc
      241 ccatctttta tgaagaatgg aaactcgata tcaaccatat aacggacgct tgggacaata
      301 agggtaacat tataattgga aatgatgtgt ggataggtta tgattctata attatgtcag
      361 gtgtaaatat tggtgatggt gcaattattg gaacaagagc agtagttact aatgacgtct
      421 caccgtattc tattgttgga ggagtacctg caaaaattat aaaaaagaga tttaataatg
      481 atacaatttc aaaattatta gaaattaaat ggtgggattg gccatataaa aaaattcaat
      541 caaacattaa gcatattcaa tcaggaaata ttgataaatt agtttaatat tttataattt
      601 atttatatta aactttactt taataat
//
